LOCUS       DAORFF010000001        27608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_656_length_27608_cov_3.898632, whole genome shotgun sequence.
ACCESSION   DAORFF010000001 DAORFF010000000
VERSION     DAORFF010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 27608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27608
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_656_length_27608_cov_3.898632"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            570..1694
                     /locus_tag="PKK76_00005"
     CDS             570..1694
                     /locus_tag="PKK76_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNL66870.1"
                     /translation="MKKAIFFGLALSLTGIMTSANATVRKTANPVVFAHGLAGWDNIL
                     GAFYFGDNNGNFVGDPCDELLEVLCNGGLNSAQKAYAASVQPFQSSEVRGTQLADKIQ
                     SYMTSVGATYVNIIGHSQGGIDARKAAVVLKQRKGFAVVKTLVSVSSPHRGSPVAKYI
                     LDLKPGVVSVVDALAQIFGNVIYGSGNDGYAAAKQLVYNDYSSTDGVTTGMKAFNTAY
                     ASYTTAVSNRLASVITAQQGLDVNPALYILSKGFYDVDGDGASCAVASDDCDNDGALG
                     KGNGSRTDTDDDGLVGINSQQMGYRLKYTECFACDDYVTQDTSLGYLTDLNNPTSAQQ
                     SSHADVLNQDHLDVTGVGPDTMDENEFYAAIFDYMSANGG"
     gene            1810..2898
                     /locus_tag="PKK76_00010"
     CDS             1810..2898
                     /locus_tag="PKK76_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL66871.1"
                     /translation="MLRRLVLPLAVSIIPLLAGCMAAFGGSPTGNILDRIRSSPQYHE
                     KKFENEPPVAMIVEGKYFEMLRGEAFGGQVRTPPRPLPVVHPDVKSFSDPPAPGLRAI
                     WFGHASVLLEVDGVRIFTDPILSDRASPFENIGPQRFFSPPLSLEELPRIDAVVISHD
                     HYDHLDMKTIKFLATRGTMFYVPLGVGAHLTAWGIADSQVHEMDWWERLPLKSVEFIC
                     TPAVHYSGRGLFGGNQTLWSSWTIVGPQHRVFHSGDSGFSPHFKEIGQRFGPFDLTLI
                     KIGAYDPTWIYIHMDPEQAMSANVALGGRRMLPIHWGTFNLQIHSWDEPILRTKAAAE
                     SSHQDLVTPRPGEILDADKPYSSAEWWK"
     gene            complement(2908..6954)
                     /locus_tag="PKK76_00015"
     CDS             complement(2908..6954)
                     /locus_tag="PKK76_00015"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNL66872.1"
                     /translation="MEQPGLDQFLPAASSWFEQTLGQPTRIQERSWEALKSGAPALIS
                     APTGSGKTLAAFFSILDDLFRKSLAGNLAQESTVLYISPLKALSNDIEKNLRIPLDGI
                     ARELAARGTPLSSPIEVMVRTGDTTPAERARMMKQKPHIIVTTPESFYVMLTSERGRS
                     MLSTVDTLIVDEIHAIVSSRRGSHLALSIERLAALTRKPLQRIGISATVKPLDRVAEF
                     LGAKKEVRILDEGHNRPMDVKMITPDVALDAVMTQEGWAQIHEKLIELMKSARTTLIF
                     VNNRRLCERLARQLSDLIGQDLVGAHHGSLAHDRRRNAEQALKAGTLKVMVATSSLEL
                     GIDIGSVDLVVQIASPRSIHGFLQRIGRAEHHKDGVSRAVLVPLTRDDLVECAALLRA
                     IKQGKLEEIEIPQAPLDILAQQLVAELACRSMTADQAFDLVRSANPYKTLERNKFEQI
                     LEMLSDGYSLRFGRRSRYVFYDRALGTLEARRGARLSAITNGGAIADNFEYEVVLADE
                     GAFLGTVHEDFAIESMAGDVFQLGNQFWKIDRITTGKVLVSHAPNSTPTMPFWIAEAP
                     GRSDELSVAVSNLRSDSQPRMQEGLEGFAEWLEEETGLGGAAATTLFEYLREGVLVLG
                     QMPTRNKIVLERFFDEAGDSHIVLHSPYGTRINRAWGLALRKRFCRKFNFELQAAATD
                     DAVLFSLSKTHSFALGEVFSYLKSEIAEKILVQALLAAPMFEIRWRWNASRALAILRQ
                     YGSRRTPPQIQKSQAQDLVALVFPDQIACAENLAGDREVPDHPLVEQTIRDCLTEAMD
                     LAGLKKLLAEIESSVIETIPVDTAAPSVFAHEIINARPYAFLDDAPLEERRTRAVRTD
                     MHTTQEVDLPSQEIIDLVRADCSPAPRSQAECYDQLCSLGIFRAEDACDSPFLQELKE
                     AGRIQRMMIGGREFYASAEMAPHARTLSDLEDSRQPSEVTSLESAISQPIFDRILRGH
                     LEKVGPVSVVDLGQRLPFSTALLTQAMIRFENEGWLFQMKTSGGIVFCERSFLARLRH
                     YQKAYGRRQEFTLSQYSSFLIKWQHAHPDTFVAGEEGLALVLEQMQGLSMSADAWHAV
                     LKMRVTDYNPRMLDSMFLSGRFVWIRAITPGDPPRKTISRFVPLMILSRANLSLVKNP
                     ETSGLSHYAQTVLEFIRRQGAVFFFDLQAQAGLFEDHLISGMKELVARGLLTADSLAS
                     LSSLFPDRKKARYLRRGVGIAASGRWSLLRENETPQDQDIMIARILLRRYGVIFRYLA
                     EKETLNLPWSILVRTLRRLEMQGEIRGGRFIREAWGEQFAMPEALTLLKETAEGKPED
                     APALSKTDPVRAVEKIMQASRTSDTTAK"
     gene            complement(7006..7374)
                     /locus_tag="PKK76_00020"
     CDS             complement(7006..7374)
                     /locus_tag="PKK76_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66873.1"
                     /translation="MRTLLLALTILAIVHCGPNKKDSYYCDITSQSLDTCDGKTIKVT
                     ANHSDRIQQHPVISPDKQSYWDVNGRDWIFVSKNAIDCKTGIVVTGRLDAKVGPCDPT
                     AQNHNQYCGTAVYVDNWKCQ"
     gene            complement(7469..7909)
                     /locus_tag="PKK76_00025"
     CDS             complement(7469..7909)
                     /locus_tag="PKK76_00025"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HNL66874.1"
                     /translation="MNPAPHLKPEERVVLFDGVCVLCTWWTRFLTRYDVNHKFLLCSI
                     QSPEGLDILAWAGIPVDDVNTMLYVSNNTAYTRSDAFIEVMGQLGLPWSLARILKIVP
                     RFFRNACYNFIAHNRYRFFGRSDVCMMPTPENQSRFLKSQTAKS"
     gene            complement(8002..8508)
                     /locus_tag="PKK76_00030"
     CDS             complement(8002..8508)
                     /locus_tag="PKK76_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNL66875.1"
                     /translation="MVRRSAKEIPDSRDLRLAWLSCLALNLKRTARLVSAAYDDAVRP
                     SGLRSTQFSILATVAVNPDVSIAQLADWVDADRTTVQRSIGIMQRNLWITVEKAEQGN
                     LRRLKLSAKGQKKLAEAYKLWEQAQKQMVDILGTEASRRLLHDLSRARKKTREKLVTQ
                     SESVASIS"
     gene            complement(8508..8999)
                     /locus_tag="PKK76_00035"
     CDS             complement(8508..8999)
                     /locus_tag="PKK76_00035"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNL66876.1"
                     /translation="MPATESKELVQGQDSTGSEIFHKMFDSFLEETAAAGVQIPPNSI
                     LESGAVFLEYESRTRLVSEFPAQEKHANPLGMLQGGIIGALIDDTMGPLSFAAVRGPT
                     TTLNLSINYLRSVKCPDRVRVEARVTGRGRRVLHLEADVFDSRGRLVAKSTSTVLSLK
                     EGG"
     gene            9123..9911
                     /locus_tag="PKK76_00040"
     CDS             9123..9911
                     /locus_tag="PKK76_00040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNL66877.1"
                     /translation="MKRTLLALLLVFAFVACQKNHVRGEEVTYKFGDQTLKGLLAYAP
                     EQTGKRPSVLVVHEWWGNNDYSRKRAMQLAELGYVALAIDMYGDGKIAANPDEAGKMA
                     GGVMKDFEKGKGRFAAALDFVKQQPQVDPTRIAAIGYCFGGGVVLNMARQGADLKGVV
                     SFHGGLDPVKPAKKGEVKARILVAHGGADSFVPMEKVDAIKKEMSEAGADLTVTVYPG
                     ATHVFTNPDADKYAKEFKMPIAYNAEADKKSWEEMANFLSSVLK"
     gene            complement(9964..10299)
                     /locus_tag="PKK76_00045"
     CDS             complement(9964..10299)
                     /locus_tag="PKK76_00045"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNL66878.1"
                     /translation="MIKASSAHFSEQVRDIARFGRLLSHPARPRILKLLAKREFMTCG
                     EIVEALPFAQATVSRHLRELTSGGLIRLDVEGLRSFYSLDNRAASRMQTCYQEFFRMI
                     ASKETGGSR"
     gene            10468..11904
                     /locus_tag="PKK76_00050"
     CDS             10468..11904
                     /locus_tag="PKK76_00050"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNL66879.1"
                     /translation="MRFTTLYFLFFFALVYVVFWIIPRRFRPYLIFVASLGFYAAWSW
                     QFAVHLLLVLLVNYLALIRLLGRPRRSTLALIVLLNVVNLVFFKYFYFILGTASQAVG
                     VIGPERLDAWLEHNLGVSHIVLPLAISFYTFQLIALQVDAYRGKIPERPSIIEYLMFS
                     LYFPHFVAGPIMRHSDFFYQLRNLKPDETRMLDGLLLVGAGLVKKVVIADNVDFVLKP
                     VFANPGGYDWSTNLMAALGFAARIYCDFSGYTDIARGSSKMLGLDIPENFKAPFLSAS
                     VGEFWNRWHVTLMMWLRDYVYIPLGGSRRSPARNYANLILTMALSGLWHGASFTFLLW
                     GLWNGVFVSIEKLLGVRTEFEPGFIRDGFVSSVARLAFRFGGMVYAYLVFALGLILFN
                     SQSLQSAMNMYGQIFFLRSGAEWPHTEYVFNIVAITLLFNIVQSRKQFHIPVGWKSYP
                     VLGALGLVVLLLLGSFPPGTTDFIYFQF"
     gene            11914..13050
                     /locus_tag="PKK76_00055"
     CDS             11914..13050
                     /locus_tag="PKK76_00055"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNL66880.1"
                     /translation="MEFLKNPFLVRPFIILGIVLGLDKLLLLQAVREYTTSYMHIEDH
                     FYDTREDLLNHLKEHYTARKAAGEKLGLIFGTSRSGEFDSAEISKIAPKTYTYNFSAP
                     QAGHSYHAYWLQRILEQNIRPDFVLLESDPITLSPNANRFPIKHSLDISFLLKHIDFD
                     SDASPWRNGGGFSTEHFERFLLSRMFAAYRFPVDLRAAARNRESHIMPVGPGIQMVTG
                     LEIRRAMLAQMKEASIRNLGGIPNPHALELPENLMNRDAETMASLHLPRPYHTGRAQT
                     RLFREMLEGLARESIPTVVFWPPVVSRFREIMRETGAQDMARSGTTEIIERLGKDYPK
                     AKLRFVEADSKGMQCRIFFDSIHIAGKCYPELTKLLLKEIQSDL"
     gene            13056..13766
                     /locus_tag="PKK76_00060"
     CDS             13056..13766
                     /locus_tag="PKK76_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66881.1"
                     /translation="MHRAILVLTIACCSCNSVKHECNPLPPAPHSVAVLGDSRIFDPT
                     PKNFAGFANYLNQDATFKYFRNTTFTSEDSDPRTSTRTPGSDIYSSVGNTTAYALHNL
                     PRVLAGPHDAMLIHLGVNSTGDPIGASRDLAAIAESARAKSWQVYIVTVGPWRGYPTW
                     TQLYQDGTDGINRWIRDQGVRYSVIDVQPVVEDREHLQYLRADYTWDKLHYTSIAHSA
                     IAADWISHIKTKGCGWDL"
     gene            13869..14993
                     /locus_tag="PKK76_00065"
     CDS             13869..14993
                     /locus_tag="PKK76_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66882.1"
                     /translation="MKFQLPDLSPLAAIQALAERVGAVSFAIQTSWGPFFVNLDRIVI
                     ISIFLILAGAALLILWAVMRSYAPAPETEPEIESIATAEPVEAPAEIPELQTISRQDA
                     LRIRPEFFMRKVSDELSRHLSFASSIDELLRAFLIHASSVCDGILTVFLRNEQGDMVP
                     RLRSQGKIVLGGDFSRVLSETNVGHTGFEILASGKPVMSEDGRQAAFSIDSPTEIAGF
                     LLLESEHASDLKVLSRLHDMCYQFSLFMYEKIHQIENQAHSGTSGRLQLESALRVLSQ
                     ASAAFSLTICEFSEGKPDISGDLIFCEGNRLYVLGGVESERTLDARFASWIHRCGPAS
                     IGIAVSMGQEEPAKIFDRALRALENAKAAGLNHYRILQAA"
     gene            14993..15493
                     /locus_tag="PKK76_00070"
     CDS             14993..15493
                     /locus_tag="PKK76_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNL66883.1"
                     /translation="MDPLLDEKVVLTLGRAFTEHMKNTLFVSAEKEAYGPSKNEGICA
                     ESCSGIGFTGELNGMMYLCMDGYTKMKLLPRIAERYQIDAGQRGMADSVLLEFSNQIA
                     SNILSELRDGGYHLELQPPESLNHKIVPIDLKRNRQYILIFFLRDRRMKRYLGRATLV
                     LTLEKY"
     gene            15556..15840
                     /locus_tag="PKK76_00075"
     CDS             15556..15840
                     /locus_tag="PKK76_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNL66884.1"
                     /translation="MKQVASTDELQQLVNRLSRAQGQLEGIKKRLVAGAQPDCKQTIH
                     QLKAAHRAITEFAEAYVLHYFEQCVTESDQPPDRKVEKNLREAISAAFTL"
     gene            complement(15830..18985)
                     /locus_tag="PKK76_00080"
     CDS             complement(15830..18985)
                     /locus_tag="PKK76_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNL66885.1"
                     /translation="MKSAQPVKEPNPGFAGSLARNFLHSHLTPIIAVASLVLGIASVY
                     LTPKEEEPQISVPMIDIRTNLPGYEAHEVEQKVTEVIEKAVWGLEGVEYVYSASSPHS
                     SLVTVRFKVGEPVEPSLVKVHHKIMEIRSSLPQEASQPLVKSYSIDDVPFLVLTFQSS
                     TRDDFSLRSVVSPLARDLAGTPDISRVDIYGGRKQSIRVVVDPARSSAAGVSILDVAH
                     ALKANHEGVLSGKNWGKDNVVSVEAGGLLSTAQDVARTGVGQRAGLVVRVGDVATVTD
                     GPEERSRLSYVMDHTGKSNAVSLSFAKRKGTNAAELSVDVMKRAERFASTLPSDIHMT
                     VMRNYGQSAKDKSSELIEHLIIATISVGVLIAITMGLRASAVVSLTIPVTLALTLGIY
                     YFMGYTLNRVTLFALIFSIGILVDDAIVVVENVERHLRESPHLSAAQATLRAVAEVGN
                     PTILATFTVIAAILPMMFVRGLMGPYMKPIPVGASFAMILSLLVAFIVTPWAAVRLLK
                     KHTHSETKASKLDALYDKVMRTLLSKKKSALVFGGVTVFLLLASMGLIASKAVKVKML
                     PFDNKNEFQVLLDYPASTPLEKSSAESVLLAQELLKHPDVERVQIFAGESAPYSFSGL
                     VKHTFLRAQDYKTDLQVVLKDKDHRKKKSHEIIEEIRPRIADFARAHNALSKVLEIPP
                     GPPVMATLVAEVYGPDNLSREKAARTVQQIFAAEKSVVDLDYSWRPERDRRVYNFNVN
                     GGALLGINASDASQASLLTFSETPIVSLAESNSPEIKTVDLSLAQSARSGASPFRGQT
                     IATREAGTANLDQVFGNPQIKTFETLHRKNLKPVSYVVSELSGAEEAPVYGIMSLGPK
                     IPFQLQTVDVPWNMDQPVVKWDGEWYITYEVFRDLGLAFAAVLVLIYVLVTGWFKSYA
                     VPLVIMAPIPISLIGILPGHAAFGAYFTATSMIGFIAGAGIIVRNSIILVDFIEHQIE
                     EGAELKEAVIQAGITRFRPMLLTAAAVVVGTAVILFDPIFQGLALSLMFGEVAATLIS
                     RIAVPVLYHWFIGKSRAAIRA"
     gene            complement(18982..20361)
                     /locus_tag="PKK76_00085"
     CDS             complement(18982..20361)
                     /locus_tag="PKK76_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNL66886.1"
                     /translation="MKQIWLPLAILWSIVAFPLRAEERLTLGEIWASVHGKSNAQKAA
                     ALELQASSIEKDRREWHWAPRIYAEGRAFGTNDPVMSFSSMLQTRNLKSADLSVPSPL
                     LSPQAYLNPLSVLAPDTLNHPVSSGFYRGILGIELPVYEGGSGQARVDAAGSAAKARE
                     WELQATVKQEYAAVAQAYAEILIRDKEDADLGILNQTLTAILSRYQLGSAANPVGRSG
                     YLGMQTLRNRIQTTLANNKMRKNAARSLVEALANEIPADWNIQSEEITPFADRYMALS
                     DTTGDSERVRAMDAMADSIAYKEVFEQAKLLPQAGVFGEASATGGRRGVGDAYYAGFF
                     VRMNLLSPQDYGGATQAKKEAEAARLRAKEARATEVAQARQLTSQEAILRQNIKLLDD
                     SLIMNQEQIYVSERLFVSGAINALQMAEILSRRADLISARYESEAELIRTRAARFILS
                     GKLPSGETK"
     gene            20489..20710
                     /locus_tag="PKK76_00090"
     CDS             20489..20710
                     /locus_tag="PKK76_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNL66887.1"
                     /translation="MYCAQTGSWYLERIIWLVAGVFTSVGTALAFFVSPWWLILPALV
                     GVNLIIFSLTGFCIMANILVMLGAKSRVA"
     gene            20735..22372
                     /locus_tag="PKK76_00095"
     CDS             20735..22372
                     /locus_tag="PKK76_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNL66888.1"
                     /translation="MKNRKIVIIGGVAGGATAAARARRVDEHAEITLVEMSGYVSYAN
                     CGLPYYIGGDIKERSKLLLQTPKGFHDRYRVRVLTHTEALKIDREKKEVLVRGPDGEQ
                     TLSYDSLILSQGGSPVVPPLPGIPSQHAFTLRNVEDTDAIHDFVEQAKPRTAVIAGGG
                     FIGLEMAEALHSRGLSVQVVEKFNQLMPPLDPEFAEFVRQELERNSIHVHLNTGLASV
                     DQHEVRLEDGQVLPADLVILSMGVRPQTSMAKEAGLEIGPTGGVLVNGAMQSSDPSIF
                     VVGDMAEIVHRITGKKVRVPLAGPANRQGRIAGSNAAGARMTYAGAMGTSVVKICERT
                     AAATGLTEKAAKEAGIQTSSVFVHANHHAGYYPGAKQLTLKVVFETKTGQVLGAQAFG
                     SEGVDKRIDVIATAIAGKLTVEDLESLDLSYAPPFSSANDPVNTAGFIGANSRSGFSP
                     VVSPDVFQTIDKSEIAILDVRDDAETARGFVAGAIKIPLPQLRDRLNEIPRDRPVYAY
                     CFSGMRSYLAARILMQNGFEKVYNVAGGYRSIMRMAV"
     gene            22382..22726
                     /locus_tag="PKK76_00100"
     CDS             22382..22726
                     /locus_tag="PKK76_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNL66889.1"
                     /translation="MRMEYWIYLIPVAIILWMIVRPGHAKKTEVKTMIESGSLIVDVR
                     SPQEFASGAYPGAINIPVDQVQARIGEFGDKGRPIVVYCQSGGRSQMAKQMLEAAGYK
                     KVVNGGGLRDMP"
     gene            22723..23232
                     /locus_tag="PKK76_00105"
     CDS             22723..23232
                     /locus_tag="PKK76_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66890.1"
                     /translation="MTWIAKRPGVAVEFIAKIFRRFSQSAAPLVEILSWSPRLLIAAA
                     LFEVFAPRNKKIARELKLIRLRVSFQVQCPWCVEMNSTDLKGANITEEELRFLSQGGP
                     VPSSLLPREMNVIRMAGVMSQSPVRMPQDLAHALDRDFSRSEIVTMTWTAAQVNFFAR
                     LFQSWVRNP"
     gene            complement(23261..24568)
                     /locus_tag="PKK76_00110"
     CDS             complement(23261..24568)
                     /locus_tag="PKK76_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNL66891.1"
                     /translation="MKHTAVLIGAGNRGQIYAKYAKKHDLKITAVADPDPLKRQRIAA
                     LHEIPPSQQFSSMEHLLRGKRLADGAILATMDSMHATPAMKALKLGYHVMLEKPMGLT
                     IRQCEEVAAISQKQKKSVLLCHVLRYTSLFARIKDILSSGTIGNIASILHTENITPFH
                     MAHSYVRGNWRNSKTSSPLILAKCSHDLDLISWFADSRAIRVSSAGGISHFRPENKPV
                     DAAARCLDCRSVCPHNAYDLYVRATPMLRSFALSRSAAGKAARLLLLFPFLKRLIPGT
                     DPWTHWPTSTFSDGSKDQTIAALKDGPFGQCVYSGLNDQVDHQETWIEFENGTTASFR
                     FNGLAPQEGRTLRVDGSRGCLHATTMNGGSLKVVLHSGRIVRFKENFSLFGHAEGDEK
                     IVLEFKSVLDGHPGRSDARSSLESHRIAFAANESRLSGRVVKI"
     gene            24646..25521
                     /locus_tag="PKK76_00115"
     CDS             24646..25521
                     /locus_tag="PKK76_00115"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNL66892.1"
                     /translation="MRFVTIVLITLALFGCKQKSETKPSQEASAPVASEQAMSRGAAA
                     KSDDGKLVQDEDVVGGQPAQPGQALGHVGLVAPVGAPGRLLEYNVAVHYRSADLLKAR
                     ALLYSIASRRGFLRSSSASAESSQVSVEMAVRSTELYDTLKDLDAAGDLLTENISVTD
                     HTEADYGNSLKVKRENLRGARRTQAMTGDAAARNWAEREAALERSEDSSDQAQLEKWK
                     IQDHVNWATISVQVSGPDLPAPIRVPSYRNAFVGLLNVILELGYIAIYILPFLLIGLL
                     LIAKRRTWLGWIKRG"
     gene            complement(25554..26039)
                     /locus_tag="PKK76_00120"
     CDS             complement(25554..26039)
                     /locus_tag="PKK76_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNL66893.1"
                     /translation="MQNSGSPEKDESPFKHESADDSPGFLLYKITVLWQQKLGAIFAS
                     FGIYQTQYAILASLKYFEEKGEASTQSHLVEHARIDKMTLSKAVRALEDAGYVSRSDS
                     KKDARAVSVKLTARGRKLVSSAIRAIEQADEEFFGCLNQETRAQYKSIARTLIENNSK
                     R"
     gene            complement(26029..26451)
                     /locus_tag="PKK76_00125"
     CDS             complement(26029..26451)
                     /locus_tag="PKK76_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HNL66894.1"
                     /translation="MWSKSYSKKVKGIKASQVWKVWSNVDEWHTWQKDTDYAKLDGPF
                     QAGSTFILKPKGGPKVRIEIVEAHENQGFVDLTRFPLARMYGSHQFVEKNGELEMTTT
                     MSVEGPLAFLWRKIVAEDVANGLEAQTEWLIQKAANAK"
     gene            26656..27450
                     /locus_tag="PKK76_00130"
     CDS             26656..27450
                     /locus_tag="PKK76_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNL66895.1"
                     /translation="MRGQILKTGSGRIFTGAALLVGALAFASFQSRAKEQIVSVRRST
                     FTEAVYGLGTIKANRVFSLRVGVPSRIRKLYVRAGDEVGQGMPLAEFDEMMPVRAPFS
                     GTISALNFNAEEIVFPQANVMTVVDSKDRYLLVSLEEQGAIRVRKGQRVRASFEGWRR
                     ERFLCEAVGLYPEEGQFQMRAHCNDLPPQILPGMTADVLIEVGKRPNVIQIPVSAIRN
                     GKVKLIRGSNDLEVGVSLGAIEGGWAEVASGDVREGDRVRVPEEAK"
     gene            27447..>27608
                     /locus_tag="PKK76_00135"
     CDS             27447..>27608
                     /locus_tag="PKK76_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNL66896.1"
                     /translation="MISIALRQMMARKRQTALTLLGIVFGSTAYIVMSGILLGFREYL
                     VDQLINNDAH"
BASE COUNT         6427 a         6803 c         7217 g         7161 t
ORIGIN      
        1 atcgtcgtct ttatctccca ttcccactgc attcttgatc gcagtgcacg aggacgatag
       61 aatcaagagc agggccaggc caatgatcca gaatttcgat ttcatatgtc agactccttc
      121 gcggcctcgc atcgccgccg aacgtctcta taagacgaga tggcgtctca gaatcaataa
      181 caaatcgcaa taaaaaatga acgccgcgcg aaaatgagcg tcaccgcttg ctttcggaac
      241 caatcggtcc cagtgtagtt gctcttgtgc gacactgcag gcttaccctc caggttcctg
      301 gaaattccac gctcctggaa tccagggatt cggaattctt ggcctaagct tccgattcat
      361 aggcgattct attgccccgg aatggatgaa tctgtttttt gaccgcacgg ttccgatcgt
      421 cattggcaaa tactgttcgt ttttgcggcg cattctgtta ggcgttagtt gtacgctcgt
      481 tcttctcaaa attgtatgtt ttcccggctt tgtggtacaa tccgtgcttt cctcatgtcc
      541 agtgtgattt cgcacaggag gagtttagaa tgaagaaagc cattttcttc ggtcttgcat
      601 tgagtctgac cgggatcatg acatcagcca atgccacagt ccgcaaaaca gccaacccgg
      661 ttgtattcgc gcacggcctg gcaggctggg ataatattct gggggcattc tatttcgggg
      721 acaacaacgg gaatttcgta ggagatccat gcgacgagtt gcttgaagta ttgtgcaatg
      781 gtggtttgaa ttcggcgcaa aaggcttacg cggcgtctgt tcaaccgttt cagagttcag
      841 aggttcgtgg aacacagctg gctgacaaga tccagagtta catgaccagc gtgggcgcaa
      901 cttacgtgaa catcattggt cattcgcagg gcggaatcga tgcgcgtaaa gcggccgtag
      961 ttctgaaaca aagaaaggga tttgcggttg tgaaaacgct cgtgtctgtt tcgtcgcctc
     1021 accgcggttc gccggtagca aagtacattc tggatttgaa accaggtgtc gtaagcgttg
     1081 tggatgctct ggctcagatt ttcggtaacg tgatctatgg ctcaggaaat gacggatacg
     1141 ctgcggcaaa gcagctggtt tacaacgact acagttctac tgacggcgtt accaccggca
     1201 tgaaggcatt taacactgcg tatgcgagtt acacaactgc agtttccaac agactggcat
     1261 ctgtcatcac ggcacagcaa ggactggatg taaatcctgc actctacatc ctgagcaaag
     1321 gtttctacga tgtagatggc gatggtgcgt cgtgcgctgt ggcgtccgat gactgcgata
     1381 acgatggagc tctgggtaaa gggaacggtt ccaggacaga cacagatgac gatggactgg
     1441 tcggaatcaa ttcccagcag atgggctacc gcctcaaata cactgaatgc ttcgcctgcg
     1501 atgattacgt aacgcaagat acaagccttg gttatctgac ggacttgaac aatccgacgt
     1561 ctgcccagca gtcatcgcat gcagatgttc tgaatcaaga tcacctggac gtaacaggtg
     1621 ttggtccaga tacaatggat gaaaatgaat tttacgcggc catatttgac tatatgtcag
     1681 cgaacggcgg ctaacaaata atcccccact cagcatcgcc tgttcgcagg cggtgctttt
     1741 ttgtgtgatc cttccgcatt tgggcagtta actgtttgct tttttcggca gccgcggcat
     1801 tagaatgcca tgctacgtcg cctcgttctt cctctggcag tttcaatcat cccgctactg
     1861 gctggctgca tggcggcctt cggtggttca cctacaggaa acatactcga tcgaattcgt
     1921 tcctcgcctc aataccatga aaagaaattc gaaaatgaac ctccggttgc gatgatcgtg
     1981 gaaggaaagt attttgagat gcttcgcggg gaagcattcg gtggtcaggt gcgcacgccg
     2041 cccaggcctc tgcctgtggt tcatccggat gtaaaatctt tttcggatcc tccagcgccc
     2101 gggctccgtg ccatatggtt cgggcacgca tccgtcctcc tggaagtgga cggtgtgcga
     2161 atcttcacag atccaattct ttccgatcga gcgtctcctt ttgaaaacat tggtccgcag
     2221 aggtttttct ctccacctct ctctctggaa gaacttccca ggattgacgc ggttgtgatt
     2281 tcgcatgatc actatgatca tctggacatg aagacaatca aattcctggc tacgcgcgga
     2341 accatgttct atgttcctct cggcgtgggt gcgcatttga ctgcgtgggg gattgctgat
     2401 tcacaagttc atgagatgga ctggtgggag cgtctgcctt tgaaatcggt tgagttcatt
     2461 tgcacgcctg ccgttcacta ctccggccgc ggattgtttg gagggaatca aactctctgg
     2521 tcctcatgga cgattgtggg ccctcagcat cgagtctttc acagcggcga ttctggtttt
     2581 tcaccgcatt tcaaagaaat tggccagagg tttgggcctt tcgatttaac actcatcaaa
     2641 atcggagcct acgatccaac ctggatctac atacacatgg atccggagca agcaatgtcg
     2701 gctaacgttg cacttggtgg aagaagaatg ctgcccattc actggggaac attcaatctg
     2761 caaattcact catgggacga gccgatcctt cgcacgaagg ctgccgctga atcgtctcat
     2821 caggatctgg tcacacccag acccggagaa atcttagacg ccgacaagcc gtacagttcg
     2881 gcggaatggt ggaaataggg atcgggttta ttttgcggtc gtgtccgaag tcctgctggc
     2941 ctgcatgatt ttttccacgg cccgcactgg atccgtcttt gaaagcgcag gagcgtcttc
     3001 tggtttgcct tctgcagttt ctttcaagag tgtcagagct tctggcattg cgaattgttc
     3061 tccccaggct tcgcggatga atcttccgcc gcggatttct ccctgcattt cgagacggcg
     3121 cagagttctc accagtatgc tccacggcag attgagagtt tccttttctg ccaggtagcg
     3181 aaagatgact ccgtatctgc gcagcaagat tcttgcgatc atgatgtcct gatcctgtgg
     3241 cgtttcattc tcgcgcagaa gggaccatct accggaagcc gcgatcccca cgccgcgtcg
     3301 caagtatcgg gccttttttc tgtccggaaa aagagatgag agagaagcca gactgtccgc
     3361 ggtgaggagt ccgcgcgcca ccagttcttt catcccggag atcaagtgat cttcaaaaag
     3421 gccagcctgc gcttgcagat caaaaaagaa aacggcaccc tgccgccgaa tgaattcgag
     3481 aacagtctgg gcatagtgcg aaagcccaga ggtctcggga ttcttcacta gagataggtt
     3541 ggcccgcgaa aggatcatca gaggaacaaa tcgagagatt gtctttctcg gcggatctcc
     3601 tggagtgatg gcgcgaatcc agacgaatcg gcctgaaaga aacatggagt cgagcatgcg
     3661 aggattgtaa tctgttaccc tcattttcaa gacggcatgc catgcgtccg cggacatact
     3721 caatccttgc atttgctcga gaacgagtgc cagaccttct tcaccggcta caaacgtatc
     3781 aggatgggca tgttgccatt tgatcaagaa actgctgtat tgcgagagag tgaattcctg
     3841 gcggcggccg tacgctttct ggtaatgccg gaggcgtgca agaaagcttc tctcacagaa
     3901 cacaatgccg cctgacgtct tcatctgaaa gagccagcct tcattttcga atcgaatcat
     3961 tgcctgcgtc aacagagcag tcgagaatgg taggcgctgc ccgagatcca cgacagacac
     4021 aggcccgact ttctccaggt gtccgcgaag aattcgatca aagatgggct gcgaaatggc
     4081 ggactccaat gaagtcacct cggaaggttg ccgggaatct tctagatcgc ttaacgttct
     4141 ggcgtgaggc gccatttctg cgctcgcgta gaattctctg ccgccgatca tcattctctg
     4201 gatgcgtccg gcttctttca gttcctgaag aaatggggag tcgcaagcat cctccgcgcg
     4261 aaaaataccc agggagcaca gctggtcgta gcattccgcc tggcttctgg gggcgggtga
     4321 acaatccgct ctcacaagat cgatgatctc ctgcgatggc agatccactt cttgtgtcgt
     4381 atgcatgtcc gttcggacgg cgcgtgttct tctctcttcg agaggtgcgt catccagaaa
     4441 agcatagggt cgagcgttga tgatttcgtg cgcgaagacg gagggcgctg cagtatcgac
     4501 tggaattgtt tcaatcactg acgattcaat ttcagcgagc agtttcttca ggccggccag
     4561 atccattgct tccgtgagac aatcacgaat ggtctgttct accaggggat gatccggcac
     4621 ttctctgtct cccgccaggt tttctgcgca tgcgatttga tccgggaaga caagtgcgac
     4681 caggtcctgc gcctggcttt tttgaatttg aggaggagtg cgcctgcttc cgtattgtct
     4741 gaggatggca agagcacgtg atgcgttcca tctccagcga atttcaaaca tgggtgcggc
     4801 aagtagtgcc tgcaccagga tcttctctgc aatttcactt tttaaatagg agaagacttc
     4861 accaagcgcg aagctatgtg tcttgctcag agaaaaaagt accgcgtcat ctgtggccgc
     4921 cgcctgaagt tcgaaattaa attttctgca gaatctcttt cttaacgcca ggccccacgc
     4981 tcgattgatg cgcgtgccgt acggcgaatg tagaacgatg tgactgtctc ctgcctcatc
     5041 gaaaaatcgt tctagaacaa tcttgtttcg cgtgggcatt tgtccgagaa ccagcacgcc
     5101 ttctctcaaa tattcaaaca atgtagtggc agcagcgcca ccgaggcccg tctcttcctc
     5161 cagccattct gcgaaacctt ccagaccttc ctgcatgcgc ggctgcgagt cggagcgcag
     5221 attggaaact gccacggaaa gttcatcgct cctgcccggt gcctctgcga tccagaaagg
     5281 catagtggga gtactgttcg gagcgtgcga taccaggact ttgcctgtgg tgatgcggtc
     5341 gattttccag aactggtttc caagttgaaa aacatctccg gccatacttt cgatcgcgaa
     5401 atcctcatgc accgttccca ggaacgcgcc ttcgtctgcc agaactactt cgtattcaaa
     5461 attatctgca atcgctccgc cattggtgat cgcactgagg cgagcgcctc tccgggcttc
     5521 cagtgtgcct agagctcgat cgtaaaacac atagcgcgat cgccttccaa atctaaggga
     5581 gtacccatca ctgagcattt cgagtatttg ctcgaacttg ttgcgctcca gtgtcttgta
     5641 agggttcgcg cttcgcacaa gatcaaatgc ctgatccgct gtcatgcttc tacacgcaag
     5701 ctctgccact agctgttgcg ccagaatgtc gagcggtgct tgcgggattt cgatttcttc
     5761 cagttttccc tgcttgatgg cgcgaaggag agctgcgcat tctaccaggt catctctggt
     5821 taaaggaaca agaacggctc gcgatacgcc gtctttgtga tgttctgcgc gcccaattcg
     5881 ttgtaaaaat ccgtgaatgg atctgggaga ggcgatctgc actaccaggt ccacagatcc
     5941 aatgtcgatc cccagttcca gggaagacgt agccaccatc actttgaggg ttcctgcttt
     6001 cagagcctgc tctgcatttc gcctgcgatc atgtgcgaga gatccatgat gcgctcctac
     6061 cagatcctga ccgatcagat cgctcaattg tcgtgcgagt ctttcgcaga gccgtcgatt
     6121 gtttacaaaa atcaaggtgg ttctggcgga cttcatgagt tcgattagct tttcatggat
     6181 ctgcgcccat ccctcttgcg tcatcaccgc gtcgagagca acatccggag tgatcatctt
     6241 cacatccatg ggtcggttgt ggccttcgtc tagaattcgt acttcttttt tcgcgcccag
     6301 aaactccgct acacggtcga gaggtttcac agtggcggag atcccgattc tctggagagg
     6361 tttacgcgtc agcgcggcca gtctttcaat agatagtgcc aggtgcgagc ctctgcgaga
     6421 tgagactatt gcatggatct catccacaat gagcgtgtcc acggtcgaga gcatgctgcg
     6481 cccgcgttcg ctcgtgagca ttacgtagaa ggattctgga gtggttacaa tgatgtgcgg
     6541 cttctgcttc atcatgcgcg cacgctctgc tggagtcgta tctccagtgc gaaccatgac
     6601 ttcgattggc gaagaaagag gagtgcccct ggctgccaac tctctcgcaa tgccgtctaa
     6661 cggtatcctc agatttttct caatgtcatt ggagagagct ttgagagggc tgatgtaaag
     6721 tacggtggat tcttgcgcga gattcccggc gaggctcttt ctaaaaagat cgtccaggat
     6781 ggaaaagaag gcggcgagag tcttgcctga tccagtgggt gcagaaatga gagcaggagc
     6841 tcctgacttc agtgcctccc aggagcgttc ctgaatgcgc gtgggttggc caagcgtttg
     6901 ttcaaaccag ctggaagccg ccggtagaaa ctgatccagt ccgggctgct ccatctggat
     6961 aagctaacgc cccggccgtt gaatagaaat gatttttttc gaattttatt ggcacttcca
     7021 gttgtccacg tatactgctg ttccgcaata ctggttgtga ttctgggcgg ttggatcgca
     7081 tggtcctact tttgcgtcca gtctacccgt aaccacgatg cctgttttgc aatcgattgc
     7141 gtttttgctc acgaagatcc agtcgcggcc attgacgtcc cagtaggatt gcttatctgg
     7201 actgatcaca ggatgttgct gaatcctgtc agaatgattt gcggtgactt tgattgtttt
     7261 gccatcgcag gtgtccagac tctggcttgt tatatcgcag tagtaggaat cctttttgtt
     7321 tgggccgcaa tgcacgatgg ccagaatggt tagagcaagc agtaatgttc gcatgggaac
     7381 tcctgagatt tggttcaggg gctccttcgt tggtggacag gcaatcaaaa aaactgattt
     7441 gcctgcggcg actggcccac gccgatcatc aggacttcgc tgtctggctt tttagaaatc
     7501 tgctttgatt ttctggagtg ggcatcatgc aaacatcgct tcttccaaag aacctgtaac
     7561 gattgtgtgc gatgaagtta taacatgcgt tgcgaaagaa gcgcgggacg atctttagaa
     7621 ttcgcgctag cgaccaggga aggccaagct gacccatcac ttcgatgaaa gcgtcgctcc
     7681 tggtatacgc tgtgttattg gaaacataaa gcatggtatt tacgtcatct acgggaatgc
     7741 cagcccaggc aagaatatcg agtccttccg gactctgaat gctgcaaagt agaaacttat
     7801 gatttacgtc gtatcgagtc agaaatctgg tccaccaggt gcatagcacg cagactccgt
     7861 caaagagcac gacgcgctcc tctggtttca gatgcggtgc gggattcatc tctctgaatc
     7921 aattgctttc atgaggagag tgcaactctt ttccatgaaa aacttttcat tcgagtgaag
     7981 gatgttcaga gctttgatgc ttcacgaaat cgaggcaacg gattcagatt gcgtgaccaa
     8041 cttttctctg gttttctttc gcgcacgcga aaggtcatgc agcagacgtc tcgaggcttc
     8101 cgttccgagg atatccacca tttgtttttg agcctgttcc cagagtttgt atgcttccgc
     8161 gagtttcttc tgtcctttcg cggatagctt cagtctgcgc aggtttcctt gctctgcctt
     8221 ctccactgtg atccacaggt ttctttgcat gattcctata cttctctgca ccgtcgtgcg
     8281 atccgcatcc acccagtctg ccaattgcgc aatgctcacg tccgggttca ctgcaacggt
     8341 tgcaagaatc gagaactgag tggacctgag cccggacggt ctgaccgcat catcgtatgc
     8401 agcgctcaca agtcgcgcag ttcttttcaa attcaatgca aggcagctca accaggcgag
     8461 cctcaggtcg cggctgtctg ggatttcttt cgcgcttctt ctaaccatca gccgccttct
     8521 ttcaatgaca gtacggtgga cgtcgatttt gccacaagtc tgccgcgcga atcgaaaaca
     8581 tctgcttcca gatgaagcac tcttcttcca cgtccggtta cgcgagcctc cactcgcaca
     8641 cggtccgggc attttaccga tcgcagatag tttatggata ggttgagggt agtggttggt
     8701 ccgcgtactg ccgcaaagct gagcgggccc atcgtatcat ctatgagagc accgataatt
     8761 cctccctgga gcattcccag aggattggcg tgtttctcct gagcggggaa ttcagaaacg
     8821 agtctggtgc gcgattcata ctcaagaaat accgcaccgg attcaagaat ggagttcggc
     8881 ggaatctgga ctcctgcggc tgcagtttct tccaaaaagg agtcaaacat cttgtgaaag
     8941 atttcggatc ctgtgctatc ctgtccctgg acaagttcct ttgattctgt agcaggcaaa
     9001 atgccctccc gagtgcatat gcactatatg atgtatatac actatgtcgg ctggcccgtc
     9061 cagtattttt tcatcggccg agttggattt gacgcaattt catagagagg ccagtaaggg
     9121 tgatgaaaag aaccctctta gcattgttgc ttgttttcgc attcgtcgcg tgtcagaaaa
     9181 accacgtacg aggtgaggaa gtgacttata agtttggcga tcagaccttg aagggcttgc
     9241 tcgcctatgc tccggaacaa accggaaaac gcccgtccgt acttgttgtg cacgaatggt
     9301 ggggcaacaa tgattactct cgcaagcgcg ccatgcagct tgctgagctt ggatatgttg
     9361 cgctggccat cgatatgtat ggagacggca agatcgcggc caatccggac gaggccggga
     9421 agatggccgg tggtgtgatg aaagattttg aaaaaggcaa aggcagattt gccgcagctc
     9481 tcgattttgt caaacagcag cctcaggtag acccaactcg cattgctgcg attgggtatt
     9541 gctttggtgg aggtgtcgtc ctgaacatgg ctcgccaggg cgcagacctc aagggtgtcg
     9601 ttagctttca tggcgggctc gatcctgtga aacctgcaaa aaaaggagaa gtgaaagctc
     9661 gcatactcgt tgctcatggc ggcgcggaca gctttgtccc tatggaaaaa gtggacgcga
     9721 tcaagaagga aatgagcgaa gcaggtgccg atttgaccgt tacggtctat ccgggtgcga
     9781 cgcatgtgtt tacaaatcca gatgcggaca agtacgcgaa agagtttaaa atgccaattg
     9841 cctacaacgc agaggcagac aaaaaatcct gggaagaaat ggcaaatttc ctttcctccg
     9901 ttctaaaata atcctctccg attttcaacg tcaggcgcgc attcgcgcgc ctgaatctcg
     9961 cgcttacctg gatcctcctg tctcctttga cgcgatcatt cgaaagaatt cctgatagca
    10021 agtttgcatc ctgctcgcgg cacgattgtc aagggaatag aaggagcgca acccttccac
    10081 atccaggcga atcaagccac ccgaagtaag ttctctcaag tgacgtgaga cggttgcctg
    10141 cgcgaaagga agtgcttcca caatctctcc gcatgtcatg aattcgcgct tcgcaagaag
    10201 cttcaaaatc ctgggtctcg cagggtgcga caaaagccga ccgaagcgtg caatatcgcg
    10261 gacttgttct gagaaatgag ctgacgatgc tttaatcaca tatcgactat atgcgatata
    10321 ttgttttcaa caatcagaat ttctttcgta aagtattttt tcctgacaga acagtcaaaa
    10381 actgattgcg cgaaacgcat ttattggaaa gaatttatcg agaatatata gttcctcaac
    10441 tattcaggat tgtaagggat tacggcaatg agatttacga cgctctattt tctcttcttt
    10501 ttcgcgctgg tctacgtcgt tttctggatc attccgcgca gatttcgccc gtatttaatc
    10561 ttcgtcgctt ctcttggttt ttatgccgcc tggtcatggc aattcgctgt ccatctactg
    10621 ctcgtattgc tggtgaacta tctggccttg atccggctcc tgggtcgtcc acggcgttcc
    10681 actctggctt tgatcgtatt actgaacgtt gtaaacctgg tgttctttaa gtatttctat
    10741 ttcatactag ggacagcatc gcaagcagtg ggagtgatcg ggccggagcg tctggacgcg
    10801 tggctggaac acaatcttgg tgtgtctcac attgtactgc cattggccat cagcttttat
    10861 acatttcagc tgatcgctct ccaggtggat gcttaccgcg gaaagatccc tgagaggcca
    10921 tccatcattg agtatttgat gttcagtctc tatttcccgc atttcgtagc cgggcccatc
    10981 atgaggcact cggatttctt ctatcaactt cgaaacctca agcccgacga aaccagaatg
    11041 cttgacggtc tcttgctcgt tggcgcaggt ctggtaaaga aagtagtgat tgcggataac
    11101 gttgattttg ttctgaagcc agtgtttgca aatcctggcg gatacgattg gtcaacgaat
    11161 ttgatggcgg cccttggctt tgctgcgcgc atctactgcg atttttctgg ctacacggat
    11221 atcgcgcgtg gttccagcaa gatgcttggc ctggatattc cggaaaattt taaagcaccc
    11281 tttttgtctg cgtcggtagg agagttctgg aatcgctggc atgtaactct gatgatgtgg
    11341 ttgcgagact acgtttatat tcctctgggt gggtccagga gatcgcctgc gcgtaattat
    11401 gcgaacttga ttttgaccat ggctctttca ggcctctggc atggagcaag ttttacgttt
    11461 ctgctctggg ggctctggaa tggagtattc gtgagtattg agaagcttct gggcgtgcgc
    11521 acagagttcg aacccggatt catcagagat ggatttgttt caagcgtcgc ccgacttgca
    11581 ttccggtttg gcggaatggt atatgcttac ttagtattcg cgttggggtt gatcctgttt
    11641 aattcgcaaa gtctccaaag tgcgatgaac atgtacggac agattttctt tttgcgtagt
    11701 ggcgcggaat ggccacacac ggagtatgtg ttcaacatcg tcgccatcac tcttttgttt
    11761 aacatagtac aatctcggaa gcagtttcac ataccggttg gatggaagag ttatccggtg
    11821 cttggggctc tgggtctggt tgtattgcta ttgttgggtt cttttccgcc gggaaccacg
    11881 gactttatct actttcaatt ttgaggatgc gacatggaat ttctgaagaa ccctttcctc
    11941 gttcgacctt tcatcatcct gggaattgtg ctcggattgg acaaacttct cctattgcaa
    12001 gccgtgcgag aatacactac atcttacatg cacattgaag atcacttcta tgacacgcgc
    12061 gaagacttac tcaatcactt gaaggaacac tatactgcca gaaaagccgc cggcgaaaag
    12121 ctgggtttga tttttggaac gtcacgatcc ggagaatttg atagcgcaga gatttcgaaa
    12181 atagcaccaa agacctatac gtataatttt agcgcgcctc aggctggtca ttcttaccat
    12241 gcgtactggt tgcagaggat tctggagcag aatattcgtc cggactttgt tcttctggag
    12301 tcagatccaa ttacattgtc gcctaacgcg aatcgttttc cgatcaagca ctctctggat
    12361 atttcttttt tgttgaagca cattgacttt gattctgacg cgtctccgtg gaggaacgga
    12421 ggaggatttt ctaccgaaca ttttgagaga tttctgttga gccgtatgtt tgcggcctac
    12481 agattcccgg tcgatttgcg tgccgctgcc aggaatcgcg aatcgcacat catgccggtt
    12541 ggaccaggaa tccagatggt cacaggtctt gagatacgaa gagccatgct tgctcagatg
    12601 aaagaggcca gcattcgaaa tctgggcgga atcccaaatc cacatgcgct tgaacttcca
    12661 gagaatctca tgaacaggga tgcagaaacc atggcttcgc ttcacctgcc tcggccgtat
    12721 catactggcc gtgcgcagac caggttgttt cgcgaaatgc tggaagggtt ggctcgcgaa
    12781 tccattccga ctgtagtgtt ctggccgccg gtagtctcaa gatttcgcga aattatgaga
    12841 gaaacgggag ctcaggacat ggcccggtcc ggcacgacgg agatcatcga gcggctgggg
    12901 aaggactatc cgaaagcgaa actgcgtttc gtggaggcgg attcgaaagg catgcagtgt
    12961 agaatctttt ttgattctat tcacattgcg ggtaaatgct accccgagct tacgaaactc
    13021 ttgctcaaag agatccagag tgatttgtag gaaaaatgca ccgtgcgatc ctggtactga
    13081 ccattgcttg ttgttcgtgc aactcagtta aacacgaatg caatcctctt ccgcctgcgc
    13141 cgcactcggt tgctgttcta ggggattctc gcatctttga tcccactcca aagaattttg
    13201 cggggtttgc caattacctg aatcaggacg caacttttaa atactttagg aatactacct
    13261 ttacttctga agattcagac cctcgcacga gcacgagaac tcccggcagc gacatttaca
    13321 gcagtgtggg gaacacaaca gcatatgctc ttcacaatct tccgcgcgtt ctggccggtc
    13381 ctcacgatgc gatgctcatt catcttggag tgaattcaac cggggatcca atcggcgctt
    13441 cgcgtgatct tgcagccatt gcggaatctg cgcgtgcaaa gtcctggcag gtatacattg
    13501 taacagttgg gccatggaga gggtatccaa cgtggactca gctttaccag gacggaacag
    13561 acggcatcaa tcgatggatt cgagatcagg gcgtgcgata ctctgtgatt gatgtgcagc
    13621 ctgtggtgga agacagggaa catcttcaat acttgcgcgc ggactatacc tgggacaagc
    13681 tccactatac atcgattgcg cacagcgcga tcgcggcgga ttggatttca cacatcaaga
    13741 caaaggggtg tgggtgggac ctctgaaggg ttcgccaggt ctggttttag cccggaaaac
    13801 tcagtttttc actcctttcg ccccgattgc cttcaaatcg gcatgccccc gctccgaaaa
    13861 tacaaacagt gaagttccag ttgcccgacc tgtctcccct tgcagccatc caagccctcg
    13921 cggagcgcgt gggtgcagtt tcgtttgcca tccagacaag ctggggtcct ttctttgtaa
    13981 acctggaccg cattgtcatc atttctatct ttctgattct agccggggct gcactattga
    14041 ttctatgggc cgttatgcga tcttatgcac cagcacctga aacagaacca gaaatagaat
    14101 cgatcgcgac tgcagaacca gtcgaagccc ctgctgaaat tcctgagctt caaacaattt
    14161 cgagacagga tgcactgaga attcgacctg aattcttcat gagaaaggtt tcagacgaac
    14221 tatccagaca cctttccttc gcgtccagca ttgatgaatt gctgcgagct ttcttgattc
    14281 acgccagttc cgtatgcgat ggcattctaa ctgtttttct gcgaaacgaa cagggcgata
    14341 tggttcctcg tttgagaagc cagggcaaga tagttctcgg tggcgatttt tcgcgtgtgc
    14401 tttctgaaac aaacgtgggt cataccggat ttgagattct cgcaagcggc aagcccgtga
    14461 tgtctgaaga tggacgtcag gcggcattct caatcgattc accaacggag attgccgggt
    14521 ttttacttct cgaatctgaa catgcatctg atcttaaggt actatccagg ctgcatgaca
    14581 tgtgctatca gttcagtctc ttcatgtatg aaaagatcca tcaaatagaa aaccaggctc
    14641 atagcgggac aagcggtcga cttcaacttg aatctgcgct tcgcgttctt tcgcaagcat
    14701 ccgcggcatt ttctcttaca atctgcgaat tttctgaagg gaagcccgat atatccggtg
    14761 atttgatttt ctgcgaaggc aatcgtctgt atgtgcttgg cggagttgaa tcggaacgta
    14821 ctcttgacgc cagatttgca tcctggattc acagatgcgg tcctgcgagt attgggattg
    14881 ctgtttctat gggccaggaa gagcctgcga aaatttttga ccgagcgctg cgtgccctgg
    14941 agaatgcgaa agccgcagga ctcaaccact accgtatact ccaggctgcc tgatggatcc
    15001 gttgttagac gagaaggtcg ttctgactct cggacgcgca tttaccgaac acatgaagaa
    15061 cactctgttc gtgagtgcag aaaaggaggc ttacgggcct tcgaagaatg aaggaatctg
    15121 cgcagagagc tgtagcggaa tcggttttac cggcgaactc aatggcatga tgtatctttg
    15181 tatggacgga tatacgaaga tgaagctgct tcctcgcatt gcggagagat accagatcga
    15241 tgcgggacaa cgaggcatgg cagattcagt tctactcgaa ttttcaaacc agatcgcttc
    15301 taacattcta agcgaacttc gtgatggcgg ttatcatctt gaacttcagc cgccagagag
    15361 tctcaatcat aagattgtgc ctattgatct caaacgcaat cgccagtaca ttctcatatt
    15421 cttcctgaga gatcgaagaa tgaaacgata cctgggacgg gcgactctgg ttttgactct
    15481 cgagaaatat tgaaaacgac aacatttctc aaaagcgctt gacgttcctt ttgtatacct
    15541 tactgtgtat tgtatatgaa acaggtcgca tccacagacg aattgcaaca acttgtcaat
    15601 cgattgagtc gcgcgcaggg gcaactggaa ggcatcaaaa aacgcctggt agccggcgcc
    15661 caaccggatt gcaagcagac cattcatcag ctcaaagcag ctcatcgcgc gatcacggag
    15721 tttgcagaag catatgttct acactacttc gagcagtgcg tcactgagtc ggatcaacca
    15781 ccggatcgca aagtagaaaa aaatctgcgc gaagcaatca gcgcggcgtt cacgctctga
    15841 tcgccgctct tgattttcct ataaaccagt ggtatagtac cgggaccgca atgcgactga
    15901 tcaaagtcgc agccacttct ccgaacatca aactcagtgc gagcccctga aagattggat
    15961 caaacaggat cacggctgtt cccacaacca cagctgcagc tgttaaaagc atgggacgaa
    16021 atcgcgtgat gcctgcttga atgaccgctt ctttcagctc cgctccttct tcgatttggt
    16081 gttctatgaa atccacaagt atgatcgagt ttcgcacgat gatcccggcc cccgcaatga
    16141 atccaatcat tgaagtcgcg gtgaagtagg cgccaaatgc tgcgtggccc ggcagaattc
    16201 ctatcaagct gattggaatc ggtgccatga tcacaagcgg aacagcataa ctcttaaacc
    16261 acccggtcac gagaacataa atcaggacga gcacagctgc gaaggcaagg cccagatcgc
    16321 gaaacacctc gtaggtgatg taccattctc catcccattt tacaacgggc tggtccatgt
    16381 tccagggcac gtctactgtc tgaagctgga agggaatctt tggacccagg ctcatgattc
    16441 cgtacacagg agcttcttcc gctccggaaa gttctgagac cacatacgaa acaggtttga
    16501 gattcttgcg atgcaaggtt tcaaatgttt tgatctgtgg atttccaaac acctggtcta
    16561 gatttgcggt tcccgcttct cttgtagcaa ttgtctgtcc gcgaaacgga gaagcaccgg
    16621 agcgcgcaga ttgcgcgaga gagagatcta ccgttttgat ctccggactg ttggattccg
    16681 cgagcgaaac aatgggcgtt tcagaaaacg taagaagaga ggcctgggat gcgtcacttg
    16741 cgttaattcc gaggagtgct cctccattta cattgaagtt atacacgcgc cggtctctct
    16801 ccggtctcca ggagtagtcc agatccacga ccgatttctc cgcagcaaaa atctgctgca
    16861 cggtgcgtgc tgctttttca cgagagagat tgtccggacc gtagacttct gcaacgagcg
    16921 ttgccatcac cggcggacca ggaggaattt cgaggacctt ggatagtgcg ttgtgcgcgc
    16981 gcgcgaaatc ggcgattcgc gggcggattt cctcgatgat ttcgtggctc ttctttttgc
    17041 gatgatcttt gtctttcaga actacttgca gatccgtttt gtaatcctgc gcgcgaagga
    17101 aggtgtgctt taccaggccg gagaatgaat acggagcact ttctccggcg aagatttgaa
    17161 cgcgttccac atctggatgc ttcagcagtt cctgagcaag caacacagac tctgcggaag
    17221 atttttcgag cggagtagac gccgggtagt ccagaagaac ctggaattca ttcttattat
    17281 caaaggggag catctttact ttcacagctt tgcttgcaat cagtcccatg ctcgcaagca
    17341 gtaggaaaac agtcacacct ccaaaaacaa gcgcactctt ctttttagac agcagtgttc
    17401 gcatcacttt gtcgtaaaga gcgtccagtt tgcttgcctt tgtttcagaa tgcgtatgct
    17461 ttttcaaaag tcgcacggcg gcccatggag taacaatgaa agcaacgagc agagaaagaa
    17521 tcatggcaaa gcttgcacca actggaattg gtttcatgta gggtcccatc agaccgcgaa
    17581 caaacatcat gggcagaatc gccgcaatga ccgtgaatgt tgcgagaatt gttggatttc
    17641 ccacctctgc cacagcgcgc agggtagcct gggccgcaga aaggtgaggg ctttcccgta
    17701 gatgcctctc cacgttttcg accactacaa tggcgtcatc caccagaata ccaatagaga
    17761 agataagagc gaataacgtg actcgattca atgtatagcc catgaaatag taaattccaa
    17821 gtgttagagc aagagttacc ggaattgtga gcgagaccac ggcggaagcc ctgagtccca
    17881 tggtaattgc gatcagtacg cccacggaaa tggttgcaat gatcaagtgt tctatcagtt
    17941 cactcgattt gtccttcgct gattgtccgt aattccgcat aacggtcata tggatgtccg
    18001 atggaagagt ggacgcgaat cgttcagcgc gcttcatcac gtctacggac aattcggctg
    18061 cgttggttcc ttttctcttc gcgaatgaga gagagactgc gttcgatttt ccagtgtggt
    18121 ccatgacgta ggacaggcga gatcgttcct ccgggccatc tgtgactgtc gccacgtctc
    18181 cgactcgcac aaccaggccg gcccgctggc ctacaccggt tcgagccacg tcctgagcag
    18241 tagacaggag tcctcccgcc tcaacagaaa caacattgtc tttcccccag ttttttccag
    18301 atagaactcc ttcatgattc gccttgagcg cgtgtgcgac gtcgaggatg gagacccccg
    18361 ccgctgaact tcgtgcagga tctactacaa cgcggatgga ttgctttctt ccgccataga
    18421 tatcaacgcg actgatgtca ggtgtgccag ccagatctcg cgccagcgga gagacaacag
    18481 atcgcaatga gaagtcatcg cgagtgctag actgaaatgt gagaaccagg aatggaacat
    18541 catcgatgga gtaagatttc acaagaggtt gagatgcttc ctgtgggagt gagctacgga
    18601 tctccatgat cttgtgatgc acttttacaa gcgacggctc tacaggttca ccaaccttga
    18661 atcgcacagt gactagactg gaatgagggc tggacgcgga gtaaacatac tccactcctt
    18721 ctagacccca gactgccttt tcaatcacct ctgttacttt ttgctctact tcatgcgctt
    18781 catacccggg tagattggtg cgaatgtcga tcatcggcac ggagatttgc ggttcttctt
    18841 cttttggtgt gaggtagacg gacgcaattc caagcacaag gctggccacg gcgatgatcg
    18901 gcgtgaggtg agagtgaagg aagtttcgcg cgagcgatcc ggcgaatcca ggatttggtt
    18961 ctttcacagg ctgtgctgat ttcattttgt ttctccggaa ggaagtttgc cgctcagaat
    19021 gaagcgtgcg gcgcgtgtac ggatgagttc tgcttcagat tcgtatctgg ctgaaattag
    19081 gtcggctcga cgcgaaagaa tttctgccat ctgcagtgca ttgattgcac ccgaaacaaa
    19141 gagtctctcc gaaacataaa tctgctcctg gttcataatc aaactatcat cgagaagttt
    19201 gatgttctgt cgcagtattg cttcctggga cgtgagctgt cttgcttgcg ccacttcagt
    19261 tgcgcgcgct tcttttgcgc ggaggcgagc cgcttctgct tctttctttg cctgtgttgc
    19321 ccctccgtaa tcctgcgggg acagcagatt cattcgcaca aagaagccgg cgtaatatgc
    19381 atctccgacg cctcttcttc ctccggtagc agaggcctct ccgaagactc cggcttgcgg
    19441 cagtagtttt gcctgttcaa agacttcttt ataggcgatc gagtctgcca ttgcgtccat
    19501 cgcacgaact ctttctgaat cgcccgttgt atcgctcaat gccatataac ggtctgcgaa
    19561 gggcgtgatt tcttcagact ggatgttcca gtccgcgggt atttcgtttg ccagggcttc
    19621 cacgagagag cgtgctgcgt tctttctcat tttattgttt gcaagcgttg tctggattct
    19681 gtttcgcaga gtttgcattc caagatagcc ggatcgaccc acggggtttg cggcggagcc
    19741 aagctggtag cgcgaaagga ttgcggtgag agtttgattt agaattccaa gatccgcatc
    19801 ttctttgtca cgaatcaaga tctccgcata cgcttgagct acagccgcat actcctgctt
    19861 cacagttgcc tggagttccc attcgcgagc tttcgccgca ctgcccgctg catcaacacg
    19921 tgcctgtcct gaaccgcctt cgtacacggg gagctcaatg cccaggatcc cgcgatagaa
    19981 tccagatgaa acaggatgat tcagagtgtc cggcgcgagg actgagagag gatttagata
    20041 cgcctgcgga ctgaggagtg gagagggaac acttagatct gccgatttca gattccgtgt
    20101 ttgcaacatg gaagagaaag acatcaccgg atcgtttgtt ccaaacgcgc gcccttctgc
    20161 gtagattcgc ggggcccagt gccattctct gcggtctttt tctatagaag acgcctgcaa
    20221 ctcaagggcg gccgctttct gagcattgga cttaccgtga acggaagccc agatttcgcc
    20281 taatgtgagt cgttcttcag ctctcagtgg gaaggcaacg atgctccaca ggatcgcgag
    20341 gggaagccag atctgtttca tggaggcaag aatatgcgtt gacgtaccgt cgtccactct
    20401 tatactatac caggtagggt atatgtcgcg aaagacgagt gaaaggaatg ggtctccatt
    20461 ctgcacaacc ctgcgaggac ggatgagtat gtattgtgca cagacaggaa gctggtatct
    20521 ggaacggatt atctggcttg tggcgggtgt ttttacaagt gtaggcacag ccctggcatt
    20581 ttttgtaagc ccatggtggt tgattctccc tgcactggta ggtgtgaatc tgatcatttt
    20641 ttctctgact ggtttttgca tcatggcaaa cattctcgtg atgcttggtg cgaagagccg
    20701 cgtcgcctaa cagaacgctc agtaggaagg atccatgaag aatagaaaga ttgtgatcat
    20761 tggcggagtt gcgggagggg caaccgcggc tgcgcgagcg agacgagtgg atgaacacgc
    20821 tgaaatcact cttgtagaaa tgagcgggta tgtgagttac gcgaactgtg gattgcctta
    20881 ctatattgga ggcgatatca aagagcgaag caagctactt ctccaaacgc ccaaaggctt
    20941 tcatgatcga tatcgtgtgc gagtgctcac tcatacggag gctctcaaga tagatcgtga
    21001 aaaaaaagaa gtgcttgttc gcggacccga cggagaacaa acactctcct acgattctct
    21061 cattctttcg cagggcggaa gtcctgtagt tcctcctctt cccgggattc cttcgcagca
    21121 tgcgtttaca ttgcgcaacg tagaagacac agatgcgatt catgactttg tggaacaagc
    21181 aaagccccgg actgcagtca tcgccggggg cggattcatt ggtcttgaaa tggcggaggc
    21241 tttgcattcg cgagggctat ccgttcaagt cgtggagaaa ttcaatcagt tgatgccgcc
    21301 acttgatccc gagttcgcag aattcgttcg acaggaactg gagcgcaatt cgattcatgt
    21361 gcatttaaac actggccttg cttctgtaga tcagcacgag gttcgcctgg aagacggaca
    21421 ggtcttgccg gcggacctcg tgattctttc catgggagtc aggcctcaga ccagcatggc
    21481 aaaagaggct ggccttgaaa tcggcccaac gggtggcgtt cttgtcaatg gcgccatgca
    21541 gtcttctgat ccttctattt ttgttgtagg agacatggca gaaatagttc atcgcataac
    21601 tggcaagaag gtgagagtgc ctctggccgg tcctgcaaat cgccagggta gaattgccgg
    21661 gtccaatgca gctggagcta gaatgaccta tgccggtgcc atgggtacgt ctgttgtgaa
    21721 aatttgcgaa cgcactgcgg ccgccacagg actgactgag aaggcggcga aggaagcggg
    21781 gattcaaaca tcctctgttt tcgtgcacgc gaatcatcac gcgggttact atcctggagc
    21841 aaagcagcta acgttgaaag tagtcttcga aacaaaaact ggccaggtgc tcggagcgca
    21901 ggcttttggt tcggagggcg tggataagcg cattgatgta atcgcaactg cgattgctgg
    21961 aaaattgacc gtggaagatc ttgaaagtct ggatctttcc tacgctccgc cattctcctc
    22021 tgcaaatgat ccggtgaaca cggcaggatt cattggcgcg aacagtcggt ctggtttttc
    22081 gcccgtggtt tctccggacg tgttccaaac catagacaaa tcggagattg caattctgga
    22141 tgtgcgtgac gacgcggaaa ctgcacgtgg atttgtggca ggtgccatca aaattcctct
    22201 tcctcagttg cgtgatcgtt tgaatgaaat tccacgagat cgccccgtgt atgcgtattg
    22261 tttttctgga atgaggagtt atctggcagc tcgcattttg atgcagaacg gttttgaaaa
    22321 agtgtacaat gtggccggcg gatacagaag tatcatgaga atggcggttt gaggagatga
    22381 gatgagaatg gaatactgga tctatttaat cccggttgcg atcattctat ggatgatcgt
    22441 tcggcccggg catgcaaaga aaacagaggt aaaaacgatg attgaaagtg gatcactgat
    22501 tgttgacgtt cgcagtcctc aggaattcgc aagcggagcg tatcccggag cgatcaacat
    22561 tcctgtagac caagtgcagg ctagaatcgg cgagtttggc gacaagggca gacccatcgt
    22621 agtgtattgc cagtcaggcg gcagaagtca gatggcaaaa caaatgctcg aagctgcagg
    22681 atacaagaaa gttgtaaacg gcggcggatt gcgtgacatg ccttgacctg gattgcgaaa
    22741 agaccaggcg ttgcggtcga attcattgcg aagatctttc gcaggttttc gcagtctgca
    22801 gcgcctctgg tcgaaattct ttcctggtca ccgcggcttc ttatagcagc cgccttgttt
    22861 gaagttttcg caccgcgaaa caagaagata gcgcgagagc tcaagctcat tcgtcttcgc
    22921 gtgagtttcc aggtgcaatg tccctggtgt gttgaaatga attcaacaga tttgaaaggc
    22981 gcaaacatca cggaagagga gctgcgtttt ctaagccagg gtggaccggt tccatcttcc
    23041 ttgcttccgc gcgaaatgaa tgtgattcgc atggccggtg tgatgtctca gtctcccgtt
    23101 cgcatgccac aggatcttgc gcatgcactc gaccgggatt tctccaggtc agagattgtt
    23161 actatgacct ggacggcggc tcaggtgaat ttctttgcaa ggctgtttca atcgtgggtt
    23221 cgcaaccctt gaattcgcgg ccatgatagt atagatactc ttagattttg acgactcttc
    23281 ctgagagacg agactcattt gctgcgaatg caatgcgatg gctctccaat gaggatctgg
    23341 cgtccgatct gccaggatgt ccgtcgagta cactcttgaa ttccagaacg atcttttcat
    23401 ctccttctgc gtgtccaaac aaggagaagt tttccttaaa acgaacaatc cttcctgagt
    23461 gaaggaccac tttgagtgat ccaccgttca tcgtggttgc atgcaggcag ccccggcttc
    23521 cgtccactcg cagagtgcgc ccctcctgtg gcgcaagtcc attgaatcga aacgatgcgg
    23581 tagtcccgtt ttcaaattca atccatgttt cctggtgatc gacctgatcg tttaaccccg
    23641 agtagacgca ctgtccaaag ggaccatctt tgagcgcggc gatggtttga tctttgcttc
    23701 cgtctgaaaa agtggaagtt ggccagtggg tccacgggtc tgtccctgga atgagtctct
    23761 tgagaaatgg aaaaagaaga agaaggcgag ccgccttgcc tgcagccgag cggctgagcg
    23821 caaaagaccg cagcatggga gttgcacgga cgtagagatc ataggcattg tgggggcaaa
    23881 cacttctgca atccagacag cgagctgctg cgtcgactgg tttattttcc ggacggaaat
    23941 gcgaaatgcc tccagcggag cttacgcgaa tggccctgga atctgcaaac caagaaatca
    24001 aatctaggtc atgcgaacat ttcgcaagaa tgagaggaga acttgttttc gaatttctcc
    24061 agttgcctcg aacataggaa tgcgccatat gaaatggagt gatgttctct gtatgaagaa
    24121 tggatgcaat gttaccaatg gttccgctcg aaaggatgtc tttgattcgc gcaaacaatg
    24181 acgtataacg tagtacatga cacagcagga cggacttctt ctgtttttgg gaaatcgctg
    24241 ccacttcttc gcactggcga atcgtcagcc ccattggctt ttccagcatc acatggtagc
    24301 ccagtttaag cgccttcatg gccggtgtgg cgtgcatgga gtccatcgtt gcaaggatgg
    24361 caccgtctgc caggcgcttc ccgcgcagca gatgctccat ggaactaaac tgctgcgaag
    24421 gcgggatttc gtgcagtgca gctatgcgct ggcgtttcaa ggggtctgga tccgcgactg
    24481 ccgtgatttt cagatcgtgc ttcttcgcat actttgcgta aatctggccg cggttgcccg
    24541 caccgatcaa cacggctgtg tgtttcattt tgcagataaa ttggaaagca ttcgcggagc
    24601 gtagcaaaaa aacgaacaga aaagaaaaca accggtctat tctctatgcg tttcgtaact
    24661 attgttttaa tcactctggc tctttttgga tgcaaacaga aatctgagac gaagccttcc
    24721 caggaagcat ctgcaccggt tgcttcggag caggcaatga gtcgaggggc cgctgccaaa
    24781 tcggatgacg gcaagcttgt acaagatgaa gatgttgtag gtggacagcc agctcaacca
    24841 ggccaggcac ttggacatgt gggtcttgtg gctccggttg gcgctccagg tagactgctt
    24901 gagtacaacg ttgcagtgca ttatcgctct gccgatttat taaaggcccg ggcccttctg
    24961 tattctatcg cctccaggcg tgggttcctt cgcagctcca gcgcgtctgc tgaatcctct
    25021 caggtttctg ttgagatggc cgtccggtcc actgagttgt acgacacgtt gaaggatcta
    25081 gatgcggccg gggatctttt gacagaaaat atttcagtaa ctgatcacac agaagcggac
    25141 tacggaaaca gcttgaaggt gaagagggag aaccttcgcg gtgcaaggcg aacccaggca
    25201 atgaccggtg atgcagctgc gcgaaactgg gcggaaagag aggcagctct ggagagaagc
    25261 gaggattctt cggatcaagc gcagcttgaa aaatggaaga ttcaggatca tgtgaactgg
    25321 gctacgattt ctgtgcaggt ttcaggaccg gatttgcctg caccgattcg cgttccaagc
    25381 tatcgcaatg cttttgtcgg tctgctcaat gtaatcctgg aacttggata cattgcaatc
    25441 tacattctgc cgttcctttt gattggcctt ttgctcattg cgaaaagacg cacatggctg
    25501 gggtggatca agcgcggtta acgggaaagc cggcttctgt acatagaagc cggctaacgt
    25561 tttgaattat tttcaatcag ggtgcgcgca atcgatttgt attgcgccct tgtttcctga
    25621 ttcagacagc caaagaattc ctcatctgcc tgttcaatag cgcggatggc actggaaaca
    25681 agcttacgcc cacgcgcagt cagctttaca gacacagccc ttgcatcctt ttttgaatcg
    25741 ctgcgcgaaa cgtatccggc gtcctcgaga gcgcgaacag ctttggaaag ggtcattttg
    25801 tctatccttg catgttccac cagatggctc tgtgtggaag cttccccttt ctcttcgaaa
    25861 tacttgagag acgctagaat cgcatactgc gtctgataga ttccaaatga tgcgaagatc
    25921 gcaccgagct tctgctgcca caaaacggta attttgtata acaggaagcc cgggctatca
    25981 tccgcggatt catgcttgaa tggactttcg tctttctctg gcgatccgct attttgcatt
    26041 cgccgccttt tgaatcaacc attcagtttg cgcttcgagg ccatttgcaa catcttccgc
    26101 cacaattttt ctccatagaa atgcgagtgg tccttcaacg ctcatggtag tggtcatttc
    26161 cagttcacca ttcttctcaa caaactggtg cgacccatac atgcgcgcga gcggaaacct
    26221 ggtgagatcc acgaatcctt gattttcgtg ggcttccaca atttcgatgc gcacttttgg
    26281 tccacctttg ggtttgagga tgaatgtact tccggcctgg aaaggcccgt ccagttttgc
    26341 atagtctgta tctttttgcc aggtatgcca ttcgtccacg ttggaccata ctttccatac
    26401 ttgagatgcc ttgattccct ttactttctt actgtaggac ttgctccaca taaaattctc
    26461 cgtcttttga aattagtaaa ccggtttact aaatgaccat gattcccagc cctcgcttct
    26521 gtcaacccga attcggtccg ggggctgatc cccgttttcg aagcggaaac ccgcgcggat
    26581 ccatagcggc tggaatcccc cggaaaacag aggtaaacta ttgacactaa aatagtatac
    26641 ttggtaaatg ggtctgtgcg tggacaaatt ctaaaaacag gctccgggcg aatcttcacg
    26701 ggagcggcat tgctcgtggg tgcgcttgcc tttgcttctt ttcagtctcg tgcgaaggaa
    26761 cagatcgttt ccgtccgtcg ctcgactttt accgaagcag tctatggtct gggcacaatc
    26821 aaagcgaatc gtgtattcag tttgcgtgtt ggagtgccgt cgcgtattcg aaagctgtat
    26881 gttcgtgcag gcgatgaagt aggccaggga atgcccctgg ccgaattcga cgagatgatg
    26941 cctgtgcgtg ctccgttttc gggaaccatc tctgctctga atttcaatgc tgaggaaatt
    27001 gtttttccac aagcgaatgt gatgactgtg gtcgattcta aagatcgcta tttgcttgtg
    27061 tctcttgagg aacagggggc tatccgcgtt cgaaaaggcc agagagtgcg cgcgagcttt
    27121 gagggatggc gccgcgaaag atttttgtgc gaagcggttg gtctctatcc agaggaaggc
    27181 cagtttcaaa tgagagcgca ctgcaatgat ctgccgcctc agattcttcc cggtatgact
    27241 gcggatgtgc tcattgaagt cggaaaaagg ccaaacgtaa ttcagattcc cgtctctgcg
    27301 attcgaaacg gcaaggtgaa attgatccgg ggatcgaatg atctggaagt cggtgtatct
    27361 ctgggggcca tcgagggcgg ttgggcagaa gttgcatcag gtgatgtgag agaaggggac
    27421 agggtgcgtg ttccagagga agcgaaatga tttccattgc actccgacag atgatggcca
    27481 gaaaaagaca gaccgctctg acgttactcg gcattgtatt tggttccacg gcctacattg
    27541 ttatgtcagg aatccttctt ggctttagag aatatctcgt ggatcagttg atcaacaacg
    27601 acgcgcac
//
LOCUS       DAORFF010000002        21871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_944_length_21871_cov_3.723827, whole genome shotgun sequence.
ACCESSION   DAORFF010000002 DAORFF010000000
VERSION     DAORFF010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 21871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21871
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_944_length_21871_cov_3.723827"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="PKK76_00140"
     CDS             complement(<1..1394)
                     /locus_tag="PKK76_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66897.1"
                     /translation="MTAALADELTRHKQAYLQLFRSVLAKRPMDAAAKEQLIQKLSAH
                     MDSVIRTTDDLIPRIPEFATMLGVGAAPLSTFIGTNFLQALQMVAKQQPQAAPVAPRP
                     SGKPSSFLVEIIQFSPSGLPAGSRFETAESGFLKLITPQGEVMSQSFLMSGDAGAPEI
                     SYDAAPSTPAASSPSPVQDQARGPAAPQAPRPPQGAEKSILKEILERFGNQIDIPEKL
                     VPKDLGDESAEVEIFEGEPDEEEATVSSAPASAASPGAPPAERPAAKRESSILREMVE
                     KFANDLDIHEKLVPRDLGDDSAEEDVFATQEEEAEEEPVATPVVDAPIPFPFTSYLDV
                     IKRLQEFQASGDQGAYRAWLANEASSEAKVLVAVRNFERKEKAGGAVNWSEEYYNLST
                     RLACKPAYVERVHKHIIKLSRVHALWNQLMQTARSAPPALMEDLKRLWPQIKLLFDEG
                     GTAAALSSRLQILLL"
     gene            complement(1391..1717)
                     /locus_tag="PKK76_00145"
     CDS             complement(1391..1717)
                     /locus_tag="PKK76_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002758438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66898.1"
                     /translation="MREGTSYRIRLTVAVYREKRLVYRNEMNLPTLYVRRSECRAHVK
                     KEIENRLRNSNFFLSPRVDFDLVRYADEASCNTYLRYRIVEADEAAVLAEKDFDHSGM
                     SLNQDA"
     gene            complement(1793..2416)
                     /locus_tag="PKK76_00150"
     CDS             complement(1793..2416)
                     /locus_tag="PKK76_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66899.1"
                     /translation="MRRVLVCSAIPEEQEMLGWYVARDVQLVPLICGIGNVAAAVSLA
                     EYLLSQTPDEILFVGSAGAYHSQSQESAGFRAVASNVFFQRDVSSIKGESSKPPLMPL
                     VIETRRGPIAAGIAMRSGCEDGSVNCVDSVTLIPPGSEFEGLDFENMECFGLAWVAHK
                     KNIPFTAILAITNDVGPLGSDQWRANYRAYSRELQKRILNGLTSPDL"
     gene            2422..2736
                     /locus_tag="PKK76_00155"
     CDS             2422..2736
                     /locus_tag="PKK76_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66900.1"
                     /translation="MIASLPAEKFRVIVKYALILSAIFLAASCSRKPAKEDCARYDRH
                     LAELAAHPAIEAALRSAGGRDAATAHCMELKKSQVECSTEATSLSDAAACESKKGSFF
                     DF"
     gene            complement(2737..3813)
                     /locus_tag="PKK76_00160"
     CDS             complement(2737..3813)
                     /locus_tag="PKK76_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNL66901.1"
                     /translation="MKNIYFVVGLSVLLLAIDKVALIPAFRQCCTTASSVSVFRLSLD
                     SDFTQDAAIERLRARKMRYFMNFGSSRSFGYYFNPTADQIKTTPYLSDNERAKLLQWD
                     AVNSAAPGASIVSEYVRMMQWLDHGARPDFISVELSRFSLNSSSLWLNDEIKNGIPAA
                     FAIRYAGQMPLPHSSTVVGSRVFALSRYRVGEPTAVSASIWEGLFSQITSNLSAEKVF
                     QQTTPGIPVGQESPVMLMSYNHVASEMRRTMFSHYHVDSNLRRYPFLMLARAKKENIP
                     IVFWNPAAHPVWLDKEKDCDVRDTYNLFLRELKREGGIYIDLSQDKMKCSEFSDPVHM
                     HIHCFPEMAWKKISALESAGALKL"
     gene            complement(3821..5299)
                     /locus_tag="PKK76_00165"
     CDS             complement(3821..5299)
                     /locus_tag="PKK76_00165"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNL66902.1"
                     /translation="MLYNSIEYFLFLSVLLVVYWKAPQKARNPILIVASLVFYAYWSI
                     AFLLHYVIILALNFPLFLAILKNRSKWAMRAALVLDFGNLIFFKYFYFMIRTIENFVP
                     AEYRGTLSQINPDNGEILPIIILPLAISFYTFQIVAFHVDAYRGEVTEAPGFWKYFLF
                     ISYFPQHIAGPIIRHHHLLKQLDEPRDPSKVDVHRALALIGMGVFKKVIIADTFAGMA
                     AQITRNPEKYDPAFVILGSVFFAIQVYSDFAGYSDIARGSSLLLGFELPVNFRGPFFQ
                     LSYQEHWQRWHITLSFWLRDYLYIALGGNRQGKFRSHLYLLITFILGGLWHGAGWGFV
                     IWGTLVGSLLVLERICRDLGWISVQETWGRRDQESSKSYSIRTVRNLSRMSLIFGLIV
                     AGGIFFNAGLDVSVAMRNFTAIFNPEADTKEFGLNSWHGLLFLAFLFLHWLEYQDFAP
                     VFRLREKYTHLERPVIAISAVLLMFLMSWKAAGNTSFVYFQF"
     gene            complement(5315..6121)
                     /locus_tag="PKK76_00170"
     CDS             complement(5315..6121)
                     /locus_tag="PKK76_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNL66903.1"
                     /translation="MNWFQNVLVGILFFGALALAGFFTIISEAGPFAKKGNLLVLYFD
                     NADGVKVGSRVTVLGVPAGVVTDLALIGVDKAGLPLKEDSPDIVAQKVAVTAEIRRPV
                     KFYSNYKIAVKNETILSGKIIAIDPGSVRPMENGKIPELISVLAPSTTDLQKERKSAL
                     EHRLGQKGGFVDLQGESSGDPLAGLSEMISENRTDIRRTLNNVAEITTKINQGKGTLG
                     LLVNDDELHRNANTVLTDAQIVVRELREGLEDTREQAPVNSFLRAALTAF"
     gene            complement(6152..7381)
                     /locus_tag="PKK76_00175"
     CDS             complement(6152..7381)
                     /locus_tag="PKK76_00175"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNL66904.1"
                     /translation="MAREFEIPLFYRSPILSVVKNARKNQDRHKRDFAPSVLDLGSMR
                     FKLARHFGFCYGVENAIEIAYRAIEENPGKRIFLLSEMIHNPQVNRDLTDRGVQFLMK
                     TDGTKLIPFEELRSDDIVIVPAFGTTLEMFDSLAKIGIDPQTYNTTCPFVEKVWNRSE
                     QMGSQGYTIIIHGFDNHEETRATFSHARRSAPSVMIRNMQEAIRLAAYVRGERDSASF
                     FEEFPGRYSIGFDPTKDFERIGVVNQTTMLATETQEIADYLKNAVIARAGAGNLSFHF
                     ADTRDTLCYATSENQESVYGLLESGGDLAVVVGGYNSSNTSHLVELCEKKLPTYYVKD
                     AEEILSRDRIRHLDLASGKIIETDGWLPVSSSRPVEILVTAGASCPDAVVDRVIDRMA
                     ALAGLRDRIEDALRVFA"
     gene            complement(7450..8211)
                     /locus_tag="PKK76_00180"
     CDS             complement(7450..8211)
                     /locus_tag="PKK76_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNL66905.1"
                     /translation="MFSHRFLTLLTGFLFFGGMLMIGYFTIARQKEQGLRSPEFYLLE
                     LETAEGLKVGASVQILGVPSGVVDSMYYVPPEDSGETKVLAVLAITRQVPIYSNYRII
                     TRFPTALAAKVVEINPGTRTNGAFQVQPRLLGSHELVNLKTSGIMPEAGANVLRAANY
                     DDPLYLIATSLTENRRSLRKIASNVAEITEKMNSGNGTIAALLNRGDLVNNTNEILRG
                     SLILIQEIREGVEDTRESKAAIDFISVVTAFVGLK"
     gene            complement(8211..8936)
                     /locus_tag="PKK76_00185"
     CDS             complement(8211..8936)
                     /locus_tag="PKK76_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNL66906.1"
                     /translation="MQIKRQDRIVFLRSLLANFASVRVEGAANLPASGGAVIVCNHTD
                     LSDGLIQLLYTPRPLVFLAKSELFESGGNLRISDIIEGNEFFRQFPSDILADMMNLAG
                     QFIQDVDAMPVIRGYRGVHRRDAAAYYRELAESVVKRLTAGELVAIYPEGRRSTSGEL
                     LPFKGFAARMALLARVRVIPCCIQGNTGLASPRLMEKNSPRSVIYKIGQSILPEEFPE
                     GGGKYAVKRLTQKIEARVKELSG"
     gene            complement(8967..10475)
                     /locus_tag="PKK76_00190"
     CDS             complement(8967..10475)
                     /locus_tag="PKK76_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66907.1"
                     /translation="MPEETRQPDAPIDKKRIALMEGTCGVPDRVSIRGQVVDIPVTEK
                     EMKSDWDMFLGLPRIARETIRPLQDVGMGGVRRARLQAEVFPEHRDGVSPPLWVSDTF
                     STNDNSFFSTTAQKLSSGIYAVRLTLRGIDSLRQSVADFAFMPSRQSLTLKTDVIIGE
                     GRFRVLPRDYTGWIVTSDIDQTFLDTKLDSRGGLMQTLFEMPSEKRPLPGMAELMRKI
                     TDRNIPLHFISASPHFFRRSFNALFAFHNIPVTGLRLKYLQGVFEEARKKLFESLLHV
                     TDYLQAGVGGAMDRSMKYLNSSLQSLFDQVGYKLEAILENRLIQPTGSREILMGDNTE
                     SDYFIFTLYQVLLTGRLRDDQLVDYLYHLRFHGREALTRDYSVRIAKLTTENLQIHGK
                     INPVEHVWINQAYASPDNEEAEKMIREALPEDAFKWGTIKKPIVVKGGLGFAVHALDV
                     GLIDPDQFRAVWKSMEGRKLGELELNQAFLETIVKSMPWTRYSAEQVLGYAV"
     gene            10541..11449
                     /locus_tag="PKK76_00195"
     CDS             10541..11449
                     /locus_tag="PKK76_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNL66908.1"
                     /translation="MQKKNSEPLPIFVYSSDYDMDLGPHVFPSVKFGFLYSRLVDNSK
                     FKKHKFVEPEPLTPQEAALVHSRAFLADLNSLTMSNRLHRSELPLNREIVDAFYLASG
                     GTALAARLALESGLAMNLSGGFHHSYADHAEGFCYLNDVAISVRMLQKEKKIKRALII
                     DLDVHQGNGNAKIFHGDNTVFTFSMHEQNNYPVKEQSDLDIGLETGCRDEQYLTLLSQ
                     ALKEIQEQFEPDLIYYLAGVDPYEHDRLGGIALTKRGMAERDRVVRDFLPGVPMVTLL
                     AGGYAENTLDTIDLHFQTCEVLARFR"
     gene            11451..12275
                     /locus_tag="PKK76_00200"
     CDS             11451..12275
                     /locus_tag="PKK76_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66909.1"
                     /translation="MGFLDWFKLGKEPDKRIELSGLDEVELAEELHGGPLVRDTPVPR
                     IFAKFAESEIHELMAWSGIFDALKEKGYDRTELELQYLSDQDQRIFVKEKGEILIHIR
                     LKLSSFRFRLHPGAPQRKLLYIDWLMTRHPRIRSYKKEKLFPGQELPGLGIFTHVTDF
                     ITNLALGVGAKGAFNIPEYFHDALLFHRAFQFYDPVREAHFRALIRDLRKFGARAISH
                     ALAEGRIRNSEGEVLAWSPGEMISILDPELEEMIWNPEYYTRVVRELKRVSYTMSS"
     gene            12294..13598
                     /locus_tag="PKK76_00205"
     CDS             12294..13598
                     /locus_tag="PKK76_00205"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL66910.1"
                     /translation="MIDPDRRISAGASLNSPHPPRDLAKYASQRLLRHLAENPGSPDE
                     PVSRSFAGAVLFADMQNFTGLAERLNTEDPANGVQKLAQYLDIYIGKLVDIITANGGD
                     IVKFAGDAVFAIWENENAMSQSLLAAIHCGLVIQKELHRFEVAPGELLALRVGVSAGN
                     MHELHLGGYRDRWEFLIAGLPMSESGQSATHADPGQVIVSPAAARVLSGSGLAKRHGK
                     KLLVRPAFPAELPGPLKRSDIVEGAEDAFSVYVPRALLVRSGNMRAELRPVTVLFCKV
                     RGFKFSAETSLAAVQVVMLVMQECVYRYEGSINRFGVDEKGAILLAAFGLPPLVHEDD
                     PLRGIQAARDIRTALAAIGHDASIGVATGRAFCGTVGNNTRCEYTMHGVNVNLAARMM
                     VRAETILCDENTVKAAGSHVQFEQQPDLSLNRLGGVRTFRPL"
     gene            13595..14188
                     /locus_tag="PKK76_00210"
     CDS             13595..14188
                     /locus_tag="PKK76_00210"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNL66911.1"
                     /translation="MIRDKEDRCPWCLKDPVMLKYHDTEWGVPQTKDKKLFEFLILEG
                     AQAGLSWITILKRRAGYAEAFAQFDPEKVATFPKRKIESLLGFEGIIRNRLKVESAIT
                     NAKAFLKVQEEFGSFNQYQWQFVDGKPKQNSWKAIGQVPATTKESDAFSKDLKSRGFK
                     FVGSTIMYAHMQAVGMVNDHLVGCPRHAAVSRISFKI"
     gene            14258..16375
                     /locus_tag="PKK76_00215"
     CDS             14258..16375
                     /locus_tag="PKK76_00215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNL66912.1"
                     /translation="MLFRAAILLFLAAGTLVAAPLPGKDPVLLGPYMGIAHDRGLTIK
                     EVTAPDFASEFKAAPSPIPNFGMTDDAYWTRFDLANPSPSPRSLMVEVAMPHLDDVRL
                     YVPEGRLFHEYVSGRNYPFSTRAVAHENYVFPVELPPYFQGTLYMRFEQEGTFQLPVY
                     LWDADNFHQADRLKQLVLGSYYGVAIVMLIYNLFLWIAIRERAYIYYVLAILFTHFLV
                     VFHINGFSYVWFFPESPVIAKRSYPVLLNLAGYFSIQFVQALLLTKERSPRLDRGLVW
                     LRRFALITSFFAVFASYFLAYKVTVYLALVMAVALIASATYRAIQGDRIAVYYVVAWS
                     VLVAATVLLILRTVGVAPVNFATDYGVQVAASLEVVLLSLALGERINEMRREKDRILI
                     DLREKQQSQQLAVALRQLTAKISSTLNHNELIEHVKVSLPDIVRADQVRLLLKSAKDE
                     LTIPDAESNGGSLDLAEMTRAMDKSEPSILQTDRAHLVAVPVHSRSVPRGLLLLRRSE
                     RFPQDEVDILRTVASEIGMVLENIRLFSEMHEMATVDSVTGLLSRAHFMNLASVEFER
                     SRRYKRPLSALMIDIDYFKQVNDLYGHQAGDEILTRIATLCKSSLREVDIIGRYGGDE
                     FSVMLPETPPRGARDVAERIRKRIEKESLKFTGKGDLQVTLSLGTATVDKKTDSLKTL
                     LSRADAALLEGKKAGKNRVAAAR"
     gene            complement(16377..17141)
                     /locus_tag="PKK76_00220"
     CDS             complement(16377..17141)
                     /locus_tag="PKK76_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNL66913.1"
                     /translation="MIDVVRNGHILELYIQTNDTNSMGLEFFRALGANIEEAEVDRGI
                     KAVMLSSRNEKFFSNGFDPSIFIGRSREDVYSVLQSALDASGKVLFSKKPVVCAMNGH
                     AMGVGAVLAIFSDYRILVEKKGRIGFPESLIGINFPSTSAYVLTQLVGIRTARDLLYS
                     GRALKSDEALSLGLVDESASPEEVLPRARAWCAQFENMAPESVTGIKVALRDSQRVIA
                     AELASNDLEFLAEAVMSPNGQEGMRSILEKRRPKFS"
     gene            complement(17154..19001)
                     /locus_tag="PKK76_00225"
     CDS             complement(17154..19001)
                     /locus_tag="PKK76_00225"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNL66914.1"
                     /translation="MESKQSDQRGAEEVHIPLEVVQQAGSGISGLLAASEVETTLLLL
                     NAIADGVIGLNRSGMIIYANQSARGLLGYSSAELMGRRLDEFLIPPQSIEKILDPVLQ
                     GDVVSTDETILLSRKASTMDVIITGVPLIQHGQVSGALVIFRDITPGILLNRSLKERE
                     EILRGIFYILPSGIMLMGQDGRIVRINEAARGLLSLGQAELRGEPYPATLWQAQDLDG
                     NEIPIEEAPFQTALQSGLPVVGKVLRLGYHPNERFVLMSSSPLNVPGAPESRMVVTTV
                     TDVNQQLKVQSRLETAVSLNNLLKNLLTETVPEETVDTALRGVCELMNADLALLGLLD
                     DERKQITYDHILGFPEVFRGYTRPFSGSICEEMFARKMPVTIAKYSADPRRIDALVRA
                     GAMTMLAAPILADQELIGSILMFRKVERPFESTDGESLMTLTPVLSAALYKATYERKL
                     RELATSDPLTGIWNRRVVYEHLDMEIERCRRYGASLSILVLDLDHFKLVNDTYGHQAG
                     DAVLITFSQLLRRLTRRTDMVGRSGGEEFMVILPDTSLQGALKTAEKIRVDFEHSPTQ
                     YRDLTIPATVSIGCTEYIRGESFEDFFGRVDRLLYRAKAAGRNQVVVDD"
     gene            19121..19987
                     /gene="mtnP"
                     /locus_tag="PKK76_00230"
     CDS             19121..19987
                     /gene="mtnP"
                     /locus_tag="PKK76_00230"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNL66915.1"
                     /translation="MAESAKIGIIGGSGLYGLEGLRVETEVTPETPWGKPSDAIVIGE
                     YAGKRVAFLSRHGRGHVYSPSEVPFRANIAALKMLGVEQIIAFSAVGSLREEIAPRDF
                     VLPVQIIDRTKGIRASTYFENGVVVHATFGDPFDGQLATLVKQAAARVNGLKLHADKV
                     VVCMEGPAFSTRAESQLYRSIGGDIINMSVLPEAKLAREAEIPYQMICMSTDYDAWKE
                     HEEAVTTELVIGHLNANAESARKLLAELIPLLGEGANPLKGSIKNAVITAPEKRNPEQ
                     LKKLAKLLPGTF"
     gene            19988..20689
                     /locus_tag="PKK76_00235"
     CDS             19988..20689
                     /locus_tag="PKK76_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66916.1"
                     /translation="MIRTLVAVSVLVMALPLSAQLSTDTRPTTGSTPDTPGTSNNSIL
                     PGAGTVPALQERRPTPRDPRYRPPVSVPGLKEPSNPAALYLFSGDVDAQKFMDSRNKQ
                     KKASRYDEADYDDLPAGGRERVWREKEMLRQFGVDHFPYAGPEQEEPESDLRRFQIVF
                     FISLPITAAASYGLFRAAKGQSAGGFSRGETMGVFALGLGLSGAIGAYDYYQNHLTQR
                     SANIELFHAAVTFRF"
     gene            20737..>21871
                     /locus_tag="PKK76_00240"
     CDS             20737..>21871
                     /locus_tag="PKK76_00240"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNL66917.1"
                     /translation="MDEPIIYAGSTAVDASVPEELPLVFLIEGDPALRSDVSTQIHYS
                     GYRVLAFEKIESALEECRRKVPAVMVVDLSQPEGDMAMVLAEIQMLNEHSIPIIFLSD
                     RGDIETRVRAVRFGGNAYFEKPADAGALIRAIDNTVHKKEIELYRILIVQQETETAAR
                     YAEILNSAGMMTEILTDPMRLFFSLQDFRPDLILMNLYYPDCLGMEIAAVVRQEEANQ
                     SIPIIYLTEETQMQRILPALNKGGDDFLPLPVTPDRLISSVAARVDRSRILRSLLVRD
                     SLTGLLNHTAVREQLDDLVQRAKRKNETFSFGMVDIDKFKRVNDEYGHPVGDRVIQSL
                     ARTIRQRIRRVDAAGRYGGEEFAVIFDGLDTDAAVRALDRIRRA"
BASE COUNT         5654 a         5643 c         5400 g         5174 t
ORIGIN      
        1 aggagcagaa tctgaagtct cgaggacagt gcggccgccg tccctccttc atcgaagagc
       61 aacttgattt gaggccagag tctttttaga tcttccatga gagccggggg cgcgcttcgg
      121 gctgtctgca tcagttgatt ccatagcgca tgcactcgcg atagctttat gatgtgttta
      181 tgcacgcgct ccacatacgc aggcttgcat gcaagacgag tggagaggtt gtagtattct
      241 tccgaccagt tcacagctcc gcccgctttc tcttttcttt cgaagttcct cacagcgacc
      301 agcacctttg cctcagatga agcttcattc gcaagccagg cccgataagc gccctggtcg
      361 ccgctggcct ggaattcctg aagccgcttg atcacatcca gataagaagt aaaaggaaat
      421 ggaatcggcg catccaccac aggagtagcc acaggttctt cttctgcttc ctcttcctgc
      481 gtagcgaata catcttcttc cgcagagtcg tcacccagat ccctgggaac tagcttttca
      541 tgaatatcca gatcattggc gaacttctcc accatctcgc gcagaataga actctctctt
      601 ttcgcggcgg gacgctctgc ggggggtgcg ccaggtgatg cggcagatgc aggtgcggat
      661 gaaacagtgg cttcttcttc gtctggctcc ccttcaaaga tttcaacttc agcagattca
      721 tctcccagat cttttggtac aagtttttcc ggaatgtcga tctggttgcc gaatcgctcc
      781 aggatttctt tcaggattga tttctctgcg ccctgaggag gacgcggtgc ctgtggggct
      841 gctggaccac gtgcttgatc ctgaacaggc gaaggcgaag atgcagctgg agttgaaggt
      901 gccgcgtcat aagaaatttc gggagctccc gcatctccgc tcatgagaaa actttgcgac
      961 atcacttcgc cctgaggcgt gatcaatttc aagaatccag actctgcagt ctcgaatcgc
     1021 gatccggcag gaaggcctga cggcgaaaac tgaatgattt ccacgaggaa gctagagggc
     1081 ttaccggaag gacgcggtgc aacgggtgct gcctgaggtt gctgctttgc caccatctgc
     1141 agcgcctgca gaaagtttgt acctataaaa gtagagaggg gcgcggctcc aacgccgagc
     1201 atggttgcaa actccggaat gcgagggatc aaatcatccg tcgtgcgaat gactgaatcc
     1261 atatgagcgg atagtttttg gatgagctgt tctttggccg ccgcatccat gggacgcttg
     1321 gccagaacgg atcgaaacag ctgaaggtac gcctgcttgt gtcttgttag ctcatctgcg
     1381 agcgccgcag tcatgcatcc tgattcaagg acatgccgga atggtcaaag tctttttctg
     1441 caaggactgc cgcttcatct gcttccacga ttctgtagcg cagatacgta ttgcaggaag
     1501 cttcgtctgc atagcgcacg agatcaaagt ctacccgggg agacagaaag aagttagaat
     1561 tgcggaggcg attttcgatc tcttttttca catgagctct gcattccgaa cgccttacat
     1621 acagagtggg caggttcatc tcattgcgat aaaccagcct cttttcacgg taaactgcca
     1681 cagtcaggcg gattcggtaa gatgttcctt ctctcatgca ttcacaagat cgtcccgcgc
     1741 agaacccgaa ataaagaggc agcgatgatt tttcggggtg gttttggaaa cgttacaaat
     1801 caggagacgt aaggccgttc aaaattctct tctgtaattc acgagagtac gctctatagt
     1861 ttgcacgcca ctgatccgac ccgagtggac ccacatcgtt tgtgatcgcg agaattgcag
     1921 tgaatggaat gttctttttg tgcgccaccc aggccagccc gaagcattcc atattctcaa
     1981 agtcaagccc ttcaaattca gatccgggtg gaatgagagt aacagaatcc acgcaattta
     2041 cgctgccatc ttcgcaaccg gatcgcattg caatgcctgc ggcgattgga cctctgcgag
     2101 tttcgatgac cagcggcatc agaggtggtt tgctggattc tccctttata gaagaaacgt
     2161 ctcgctgaaa aaatacattg gatgcaaccg cacgaaatcc tgcagactcc tgtgactgcg
     2221 aatgatacgc accggcagat cccacaaaca ggatttcatc tggcgtctgg gagagaagat
     2281 actcggccag gcttactgcg gcagccacat tgccgatgcc gcaaatcaaa ggcacgagct
     2341 gcacgtctcg cgcgacatac catccaagca tttcctgttc ttctggaatg gccgaacata
     2401 caagtacacg ccgcatcttt tgtgattgcc tccctgcccg ctgagaaatt ccgtgtgatt
     2461 gtgaagtacg ctctgatttt gtctgccatc tttcttgcgg ccagttgcag ccgcaaacct
     2521 gccaaagaag actgcgccag gtatgatcgc catcttgcgg aactcgcagc tcaccctgcc
     2581 atcgaagcgg ctcttcgttc tgcaggcgga agagatgctg ccaccgcaca ttgcatggaa
     2641 ctcaaaaaat cacaggtaga atgttctacg gaagccacgt ccctttcgga cgctgcagcc
     2701 tgcgaaagca agaaaggctc tttcttcgac ttttaactag agcttgagcg cgcccgcaga
     2761 ctcaagggcg ctgatcttct tccacgccat ttccggaaag caatgaatgt gcatgtgcac
     2821 cggatcgctg aactctgagc atttcatctt gtcctgactc agatctatgt aaataccacc
     2881 ctctctcttt agttcgcgca gaaacaaatt gtacgtatcg cggacatcgc agtccttctc
     2941 tttgtcgagc cacacaggat gcgcggcagg attccagaat acgatcggga tattttcctt
     3001 tttcgcgcga gcgagcatca agaaaggata tctcctgaga ttcgaatcca catgatagtg
     3061 cgaaaacatg gtacgcctca tttcggaagc tacatggttg taactcatga gcattacagg
     3121 cgattcctgg ccaaccggga taccgggcgt agtttgctgg aacactttct cagcggaaag
     3181 gttggacgtg atctgactga agagaccttc ccagatagac gcagatactg cagtaggttc
     3241 gcccactcta tacctggata atgcaaacac acgacttcca actacagtag atgaatgagg
     3301 cagcggcatc tgtcctgcat aacggatggc aaaagcggcc gggattccat tcttgatttc
     3361 atcattcagc cacagactgg aggagttcag agagaaacgc gataattcaa cagagatgaa
     3421 atccgggcgg gctccatgat cgagccactg catcatgcgc acgtattcgc ttactatgga
     3481 cgccccgggt gccgcagaat tcactgcgtc ccattgcaac agtttcgccc tctcattgtc
     3541 agaaaggtaa ggagtggttt tgatctggtc tgcggtaggg ttgaagtaat agccaaacga
     3601 cctggaactt ccaaagttca taaaatagcg catcttacga gcgcgaaggc gttcaatggc
     3661 cgcgtcctgc gtgaagtctg agtccagaga aagacgaaat acgcttacac tcgatgcagt
     3721 ggtgcagcac tgacgaaatg ctgggatcaa agccaccttg tctatggcca gaagaagaac
     3781 agaaagaccc actacaaaat agatattctt catctctgcc tcaaaattga aaatacacga
     3841 aggaagtatt tcccgctgcc ttccagctca tcaggaacat caagagcaca gcggaaattg
     3901 caatgaccgg tctctccaga tgcgtatact tttcgcgcag gcgaaagaca ggagcaaagt
     3961 cctgatactc gagccagtgg agaaacaaga atgcaagaaa caagagtccg tgccaggagt
     4021 tcagaccaaa ctcttttgta tccgcttccg ggttaaaaat cgcagtgaag ttgcgcatag
     4081 ccaccgatac atcgaggccg gcgttgaaga aaatacctcc ggccacaatc aaaccgaaga
     4141 tcaaagacat gcgcgacagg tttcgcaccg tgcgaatgct gtaagactta ctcgactcct
     4201 gatcacgcct gccccacgtc tcctgaaccg agatccatcc taaatcacga caaatgcgtt
     4261 cgagaaccag aagagaaccc accagagtcc cccagatcac aaagccccag cccgcgccgt
     4321 gccacagacc accgagtatg aacgtgatga gaaggtagag atgagatcta aatttgccct
     4381 ggcgattccc acccagagca atgtagagat agtctctcaa ccagaaagac agtgtgatgt
     4441 gccacctctg ccagtgctcc tggtaactga gctgaaaaaa aggcccgcga aaattcacag
     4501 gaagttcaaa cccgagcaag agagaagatc cacgagcgat atcggagtac cccgcgaaat
     4561 cagaataaac ttgaatcgca aaaaacacac ttccaaggat cacgaatgcc gggtcatact
     4621 tttccgggtt ccgagtgatt tgcgccgcca tgccggcaaa cgtatctgcg atgatcactt
     4681 tcttgaacac acccatacca atgagtgcca gtgcgcgatg cacatctact tttgatggat
     4741 cgcgtggttc atctaactgc ttgagaaggt gatggtggcg aatgatcggg cccgcgatat
     4801 gctgaggaaa atagctaatg aagagaaaat atttccagaa cccaggcgcc tctgtgactt
     4861 ccccgcggta cgcatccacg tgaaaggcaa caatctgaaa agtgtagaag ctaattgcga
     4921 gaggaagaat gatgatagga agaatctcac cgttgtccgg gttgatttga gaaagcgtcc
     4981 cgcgatattc tgccggaacg aaattctcaa tagttcgaat catgaaatag aaatacttaa
     5041 agaatattaa gtttccaaaa tcgagaacta gcgctgctct catggcccat ttggatctat
     5101 tcttgaggat ggccagaaag agaggaaagt tcaacgccag gataatgaca tagtgcagta
     5161 gaaaggcgat ggaccagtat gcatagaaga caagtgacgc aacaatcaga attggattgc
     5221 gagctttctg aggtgccttc caataaacta caagaagtac ggaaagaaat aagaaatatt
     5281 caatgctatt gtagagcacg agttgccttc agaattaaaa tgcggtcaga gccgcgcgca
     5341 ggaagctgtt aactggcgcc tgctcacgag tatcctcaag accttcgcgc aactcgcgca
     5401 caacaatttg cgcgtccgtc aagactgtat tcgcattgcg atgaagttca tcgtcattta
     5461 cgagaagccc cagagtaccc ttgccctgat tgatcttggt tgtgatttct gcaacgttgt
     5521 ttagagtacg acgaatgtcc gttctgtttt ctgaaatcat ttcagaaagg ccggccagag
     5581 gatctcctga gctttccccc tggagatcca cgaatccacc cttctgtccc agacgatgct
     5641 cgagagcgct cttgcgctcc ttctgcaaat cagtagtaga tggagccagc acactgatta
     5701 gttcaggaat tttaccattt tccatgggac gaacggaccc cggatcgatg gcgatgatct
     5761 taccggatag aatggtttca tttttaacag cgatcttgta attgctatag aattttaccg
     5821 gacgacgaat ctcagccgta acggcaactt tctgcgcgac gatgtctgga gagtcttctt
     5881 taagcgggag cccggcctta tccactccaa tgagagccag atccgtcaca accccggcag
     5941 gcacacccag aacagtaact ctagatccaa ccttcactcc atccgcattg tcaaaataca
     6001 aaaccagcag attgcctttc tttgcaaaag ggcccgcttc cgagatgatc gtaaagaagc
     6061 cggccagggc gagtgcgcca aagaataaga taccgacaag tacgttctgg aaccagttca
     6121 cagtgaaagc ctctttccag agtttgcctg gtcaagcgaa aacacgcagc gcatcctcta
     6181 tgcgatcccg gagtccagcc agagcggcca tacggtcgat cacacgatcc accacggcat
     6241 ccgggcaact ggcacccgcg gtcaccagga tctccactgg ccgcgacgaa cttacaggaa
     6301 gccacccatc tgtttctatg atcttgcctg aggccagatc cagatgacga atccggtccc
     6361 gggagagaat ctcctccgca tcctttacgt agtaagtggg cagcttcttc tcgcacagct
     6421 ctacgagatg agatgtattg gaagaattgt atccgcccac aaccacggcc aggtcgccac
     6481 ctgactctag cagcccatag acagattcct ggttctcgga cgtggcatag caaagcgtat
     6541 cgcgagtatc cgcaaaatga aaagagagat tcccggcccc tgctcgcgcg atcaccgcgt
     6601 tcttgagata gtctgcaatt tcctgggttt cagttgcaag cattgtggtc tgattgacga
     6661 ctccaattct ttcaaaatct tttgttggat cgaagccaat cgaatagcga cctggaaatt
     6721 cttcaaaaaa agaagcagag tctctttccc ctcgaacgta cgcggccaga cgaatcgcct
     6781 cctgcatgtt gcgaatcatg acagagggtg cgcttcttct tgcatgcgaa aaagttgcgc
     6841 gagtctcttc atggttatca aaaccgtgaa tgatgatcgt gtaaccttgc gaacccattt
     6901 gctccgatct gttccagact ttctccacaa acggacaggt agtattgtaa gtttgtggat
     6961 caattccgat ttttgccaga ctatcgaaca tttccagggt ggttccaaat gcaggaacaa
     7021 ttacgatatc gtcgcttctg agctcctcaa acgggatgag ctttgttccg tccgtcttca
     7081 tcaaaaactg gacaccgcga tctgttagat cacgattcac ctgaggattg tggatcattt
     7141 cgctcagcag gaagatgcgt ttccccggat tctcctctat ggcgcgatag gcgatttcga
     7201 tagcgttttc aacgccatag caaaagccaa agtgtcgagc gagcttgaat ctcatacttc
     7261 caagatccag cacagacggc gcaaaatctc gcttatgcct atcctggttc ttgcgcgcat
     7321 tcttcacaac ggatagaata ggcgatcgat agaacaacgg aatttcaaac tctcgggcca
     7381 taaactaagg aagcaccgcg cgcgtggcac tgcaaatgaa aaaaacaagc aatcaatggc
     7441 gaacgacttc tatttcaggc caacgaatgc ggtcaccact gaaataaaat cgatcgcagc
     7501 tttgctctct ctcgtatctt ccacaccttc acgaatttcc tgaatcaaaa tcaaggaccc
     7561 gcgcaagatt tcattcgtgt tgttcacgag gtccccccgg ttgaggagtg ctgcaatggt
     7621 tccgtttcct gaattcattt tttcagtaat ctctgccacg ttagaagcga tcttgcgaag
     7681 cgatctgcga ttttcagtca aactggttgc aatcagatag agcggatcat catagttggc
     7741 tgcgcgcaat acattggctc cggcctctgg catgataccg gatgttttca aattcacgag
     7801 ctcatgcgaa ccaagtaacc tgggttgcac ctgaaaagcc ccattggtcc gcgtacctgg
     7861 attgatttca actaccttgg ctgccagagc agttggaaat cgagtgatga ttctatagtt
     7921 ggagtaaatg ggtacctgac gagtgatcgc aagcacagcg agaacttttg tttcacccga
     7981 atcctctgga ggaacgtagt acatggaatc gactacgccc gaaggcactc ccagaatctg
     8041 caccgaagcg cccaccttga ggccttctgc tgtttccagc tccagcaaat agaattcagg
     8101 agagcgaagc ccctgctctt tctgccgggc aattgtaaaa tatcctatca tcagcattcc
     8161 gccgaagaaa agaaatccag tcagaagagt taagaatcga tgagaaaaca tcagcccgag
     8221 agctccttca ctctggcttc aattttttgc gtcaatcgtt tcactgcata cttgccacca
     8281 ccctccggaa actcttcggg caagatcgat tgtccgattt tgtaaatcac ggatcgcgga
     8341 gaattctttt ccataagacg aggacttgcc agacccgtgt tgccctgaat gcagcaggga
     8401 attacacgca ctcgagcaag caatgccatg cgagccgcaa atcctttgaa cggaagaagc
     8461 tctccagatg tagatctcct gccttccgga tagatggcaa cgagctcccc tgccgtcagg
     8521 cgcttcacca cactttctgc gagttctcta taataagcag cggcatcacg cctgtgcact
     8581 ccgcgatacc ctcgaatgac tggcatggca tctacgtcct ggataaactg gcccgcgagg
     8641 ttcatcatat ccgccagtat gtcagatgga aactgacgaa agaattcgtt gccctctatg
     8701 atatcagaga tccgcagatt tcccccggat tcaaagagtt ccgatttcgc aagaaacacg
     8761 agagggcgcg gagtatacag gagctggatg agtccgtctg aaagatccgt atgattgcag
     8821 acaatgaccg cgccacctga cgcgggaaga ttggccgcac cttccactcg aacgcttgcg
     8881 aaatttgcga gcaagctgcg aagaaaaaca atcctatcct gtcttttgat ctgcactact
     8941 gaattgccgc gggcaaaagt aagacgttag acggcatatc cgagcacctg ctccgcacta
     9001 tacctggtcc agggcatgga ctttacgatg gtttccagga atgcttgatt caattccagt
     9061 tcaccgagtt tgcgaccttc catggatttc cacactgcgc gaaattgatc cggatcgatg
     9121 agaccaacgt ccagtgcgtg cactgcgaat ccaagtccac ccttgacgac gattggcttt
     9181 ttgatggttc cccatttgaa tgcgtcttct ggaagggctt cgcgaatcat tttctctgct
     9241 tcctcattat ccggagaagc gtacgcctga ttgatccata catgttctac aggattgatc
     9301 ttaccatgaa tctggagatt ctccgtcgtg agttttgcaa tgcgaacaga atagtcgcga
     9361 gtgagcgctt cgcggccgtg aaatcgaaga tgatagagat agtccacaag ctggtcatcg
     9421 cggagtctgc ccgtaagtag aacctggtag agagtgaaaa tgaaatagtc actttccgtg
     9481 ttatctccca tgagaatttc gcgcgaaccc gtaggctgga tcaaacggtt ttcaagaatc
     9541 gcttctaatt tgtatcccac ctgatcgaag agactctgca gacttgagtt gaggtacttc
     9601 atggatcgat ccatggcacc gccgactccg gcctgaagat aatccgtaac gtgaagtaga
     9661 ctctcaaaca atttctttcg cgcttcttcg aatactccct gcaaatactt caggcgaaga
     9721 cctgtgactg gaatattgtg aaacgcaaac aatgcgttga agctcctgcg aaagaaatgc
     9781 ggggaggcag aaataaaatg cagaggaata ttacgatccg taatcttcct catgagttcg
     9841 gccataccgg gaagaggtct tttctcggaa ggcatttcaa agagggtctg catgagaccg
     9901 ccgcgagaat cgagctttgt atccaggaac gtctgatcaa tgtctgaagt tacaatccat
     9961 cccgtatagt cccggggaag aactcgaaat ctaccttctc caatgatcac atccgtcttc
    10021 agagtcagac tctggcgtga aggcatgaat gcaaaatcag caacactctg gcgaagagaa
    10081 tcaatgccgc gcaaggtcag acgaaccgca tagattccag aagagagctt ttgtgcggtc
    10141 gtggaaaaga aactattgtc attggtagaa aatgtatctg aaacccagag aggtgggctg
    10201 actccgtccc gatgttctgg aaaaacctcc gcctgaaggc gtgcccggcg cactccaccc
    10261 atacctacat cttgtagagg tcgaatggtt tcccgggcaa tcctgggcag cccgaggaac
    10321 atgtcccagt ctgacttcat ttccttttct gttacgggaa tatccactac ctggccgcgg
    10381 atagaaacgc gatcgggcac accgcacgta ccctccatga gggcgatgcg tttcttgtct
    10441 atgggtgcgt ctggttgtcg ggtctcttct ggcatgcttc gctttactgt tttacagatt
    10501 caagggccgc ttcacattga ccgaacgtac ctctggcgtc atgcaaaaaa agaactcaga
    10561 gcctttgccc atcttcgttt actcgtcgga ctacgacatg gacctggggc ctcacgtttt
    10621 cccgtccgtg aagttcggct ttctttattc acgcctcgtg gataactcca aattcaagaa
    10681 gcacaaattc gtcgaaccgg agcccctgac gcctcaagaa gccgccctgg ttcattccag
    10741 agcattcctg gcagacctca actccctgac catgtccaat cgccttcaca gaagtgagct
    10801 cccgctgaac cgcgagattg tggacgcatt ctatctcgca agcggcggaa cggctcttgc
    10861 cgcaagactc gcccttgaat ccggtctggc catgaacctc agtggtggct ttcatcactc
    10921 gtacgcagat cacgcggaag gtttctgcta cttgaacgac gtggccatca gcgttcgcat
    10981 gcttcagaaa gaaaagaaga tcaaacgcgc gctcatcatc gacctggacg ttcaccaggg
    11041 caacggcaat gcgaagatct ttcacggaga taacacagtc tttaccttct ccatgcacga
    11101 acaaaacaac taccccgtca aagaacagag cgatctggac attggactgg agactggttg
    11161 cagagatgag caatacctca cccttctttc gcaggcactc aaggagattc aggaacaatt
    11221 tgaaccggat ctaatctact atctggccgg ggtggatcca tacgaacacg acaggctggg
    11281 tggaatcgct cttacaaaaa gaggcatggc ggaaagagat cgcgttgtga gagatttcct
    11341 tccgggagtt ccgatggtca cgctccttgc gggtggatac gcagagaaca cgctcgatac
    11401 gattgacctt cattttcaga cgtgcgaagt gctcgctcga ttcaggtaat atgggatttc
    11461 tagactggtt caaattaggc aaggagccgg acaagagaat tgaactctcc ggtctagacg
    11521 aagttgaact ggcagaagaa ctgcacggcg gcccgctggt tcgagatact ccggttccgc
    11581 gcatttttgc gaagttcgca gaatctgaaa tacacgagct gatggcgtgg tccgggatct
    11641 ttgacgctct gaaagaaaaa ggatacgatc gcactgagct ggagctacag tatctctccg
    11701 atcaggatca aagaatcttt gtaaaagaga aaggcgaaat tctcattcac attcgcctca
    11761 agctttcttc ttttcgattt cgcctgcatc caggtgcgcc gcagagaaaa ctactctaca
    11821 ttgattggct gatgactcgc catccgcgga ttcgatccta caaaaaagaa aaactctttc
    11881 ccgggcagga attgcctggt cttggaatct tcactcatgt cacggacttc atcacgaatc
    11941 tggcgcttgg agttggagca aaaggtgcct tcaacattcc agaatacttt catgatgctc
    12001 ttctctttca tcgcgcgttt cagttttatg atccggttcg agaagcacac tttcgcgccc
    12061 tcatccgcga cctcagaaaa tttggagcac gggccatttc gcacgctctg gcggaaggaa
    12121 gaatccgtaa ctcagaggga gaggtgcttg cctggagtcc tggggaaatg atatccattc
    12181 tagatcctga gcttgaggaa atgatctgga atccggagta ctacactcgt gtggttagag
    12241 agctcaaacg agtctcttac accatgtcca gttgaaggat ttctggcttg accgtgatcg
    12301 atccagaccg ccgcatctca gcaggcgcat ctttgaacag tcctcatcct cccagagacc
    12361 ttgcgaagta tgcttcgcaa agacttctgc gccacctcgc agagaatcca ggcagcccgg
    12421 atgaaccggt ctccagaagc tttgccggag cggttctgtt tgcagacatg cagaacttca
    12481 ccggactcgc agaaaggctg aatacagaag atccggcaaa cggagtccag aagctcgcac
    12541 agtacctgga catttacatt ggaaaactcg tagacatcat cactgccaac ggaggcgaca
    12601 ttgtaaagtt tgccggcgac gcagtctttg ccatctggga aaacgaaaat gcaatgtcgc
    12661 agtctctact tgctgccatc cactgcggcc tcgtcataca aaaagaactg catcgtttcg
    12721 aagtggctcc aggcgaacta ctggcactgc gtgttggagt aagcgcgggc aatatgcacg
    12781 aacttcacct gggcggatac agagacagat gggagtttct aatcgcgggc cttcccatga
    12841 gcgaatcagg acagtctgcc acacatgcag accctggaca ggtcattgta tctccggcag
    12901 cagccagagt cctgtcaggc tctggacttg caaaaaggca cggaaaaaaa ttgctcgttc
    12961 gtcccgcgtt tccagcggag cttcctggtc cactcaagag atctgatatt gtagaaggtg
    13021 cggaggatgc attcagcgtt tatgttccgc gcgcacttct tgtgcgaagt ggaaatatgc
    13081 gcgcagaact gcgacctgtg actgtacttt tttgcaaagt tagaggattc aaattctcgg
    13141 cggaaacatc tctggcggcg gtgcaggtag tcatgctggt catgcaagag tgtgtgtatc
    13201 gttacgaggg aagcatcaat cgatttggtg tggatgaaaa aggagccatc ctcctggccg
    13261 cattcggcct tccaccgctg gttcatgaag atgacccgtt acgcggcatt caggcagcgc
    13321 gcgacattcg aacagccctg gctgccatcg gccacgacgc atcgattgga gtggccacag
    13381 gccgtgcgtt ctgcggaaca gtgggaaaca acacacgctg cgagtatacg atgcacggag
    13441 tgaacgtaaa cctggcggct cgcatgatgg ttcgcgcaga aaccattttg tgcgacgaaa
    13501 acacagtaaa agcggcagga agtcacgttc aattcgaaca acaaccggac ctttccctga
    13561 atcgactcgg cggagtccga acatttcgcc cactatgatc cgcgataaag aagatcgatg
    13621 cccgtggtgc ctgaaagatc ccgtcatgtt gaaataccat gacaccgaat ggggtgtgcc
    13681 gcagacaaaa gacaagaagc tatttgagtt tctcatcctc gaaggcgctc aggcaggcct
    13741 gagctggatt acgattctaa aaaggcgagc gggctacgcg gaagcattcg ctcaatttga
    13801 tccagagaaa gtggcgacat tcccaaaaag aaaaatagaa tcccttttgg gttttgaagg
    13861 aatcatacgc aatcgtctga aagtagagtc tgcgatcacg aatgcgaaag catttctgaa
    13921 agttcaggaa gaattcggga gttttaatca ataccagtgg caatttgtag acggaaagcc
    13981 aaaacaaaat agctggaagg ccataggaca ggtgcctgcg acaacgaagg aatcagacgc
    14041 attcagcaag gatctaaagt cgaggggctt caagttcgtt ggatccacca tcatgtatgc
    14101 tcatatgcag gcggtaggga tggtcaacga tcatctggtc ggttgcccca gacatgcagc
    14161 cgttagccga atcagtttta aaatctaaac taattcaatt tcgtcctgaa ttcgaaaaag
    14221 aatcgacaca acaacctccc gtcttcaggc tcatcgcgtg ctttttcgcg cagccatcct
    14281 cttgttcctg gcagccggca cactggtcgc agcccctcta cccggaaaag atccggtact
    14341 gcttgggccg tatatgggaa tcgcgcatga ccgcggactg acaatcaaag aagtcactgc
    14401 acccgatttc gcgagcgaat ttaaggctgc tccttctccc atcccgaact ttggaatgac
    14461 agatgatgca tactggacca gattcgatct ggcaaacccc tccccttctc ccagaagctt
    14521 gatggtagaa gtggccatgc ctcatctaga cgatgttcgt ctctacgttc cagaagggag
    14581 actctttcac gaatatgtat ctggaagaaa ttatccgttc agcactcgcg cagttgcaca
    14641 cgagaactat gtattcccgg ttgagcttcc gccctatttt caaggcacgc tgtatatgcg
    14701 attcgagcag gagggcacgt tccagcttcc ggtctacctc tgggacgcag ataactttca
    14761 tcaggcagac aggctaaagc aactcgtcct gggatcttac tacggcgttg cgatagtaat
    14821 gttgatttac aatctgttcc tgtggatcgc catacgcgag cgcgcataca tctactacgt
    14881 tcttgccatc ctgttcactc atttcctggt cgtgtttcat atcaacggct ttagctatgt
    14941 ctggtttttt ccggagtcgc cggtgattgc aaaaagaagc tatcctgtgc tcctgaacct
    15001 cgccggatat ttctctatcc aattcgttca ggcacttcta cttacaaaag aaagatctcc
    15061 acgcctggat cggggcctgg tgtggcttcg cagatttgct ctcatcactt ccttcttcgc
    15121 agtcttcgca tcgtacttcc tcgcgtacaa ggtgacggtt tatctagcac tggtgatggc
    15181 cgtggcactc atcgcaagtg caacgtatcg ggccattcag ggagatcgga ttgcagtcta
    15241 ctacgtggtg gcgtggtctg tgcttgtggc agcaactgtt ctattgattc ttcgaacggt
    15301 cggtgtggct cccgtgaatt tcgcaacgga ttacggcgtg caagttgcag catctttaga
    15361 agtggtgctc ctctctctgg ctcttggaga aagaatcaat gagatgaggc gagagaaaga
    15421 tagaatcctc attgacctgc gagaaaaaca acaaagccag cagcttgccg tagctctcag
    15481 acaattgacg gcgaagatta gctccacact caatcacaat gaacttatag aacatgtaaa
    15541 agtgtctttg cctgatatag tcagagcaga ccaggtgcgt ctacttctca agtcggccaa
    15601 agacgaactt acaattcccg atgcggaatc aaacggtgga tctctggacc tggcagagat
    15661 gaccagagcc atggacaaaa gcgaaccgtc cattcttcag acagatcgtg cacacctggt
    15721 cgcagtgcca gtgcactctc gctccgttcc ccgcggactg ttgttgcttc gaaggtcaga
    15781 acgatttccg caggatgaag tggacatttt gcgcacggtt gcatccgaga tcggcatggt
    15841 ccttgagaac attcgcctct tttcagaaat gcacgaaatg gcaaccgtgg attctgtgac
    15901 cgggctacta tccagagctc acttcatgaa ccttgcatcc gtggaatttg aaagatcgcg
    15961 cagatacaag agaccgcttt ccgcactcat gatagacatc gactatttca aacaggtaaa
    16021 cgatctatac ggtcatcagg ctggagacga aatactcaca cgcattgcaa ctctttgcaa
    16081 gagcagtctg cgcgaagtgg atatcattgg aagatacggt ggagacgaat tctccgtgat
    16141 gcttccagaa acgcctcctc gcggagcacg cgacgttgca gagagaattc gaaagagaat
    16201 agaaaaagaa tcactgaagt tcacggggaa aggagacctt caggtaacgt tgagtctggg
    16261 cactgccacc gtggacaaga aaacggattc tctaaaaacc cttctgtcgc gcgcagacgc
    16321 agcgcttctg gaaggaaaga aggctggcaa aaatcgcgtc gcagctgcca gatagttcag
    16381 ctaaactttg gtcttctttt ttccagaatg gaacgcatac cttcctgacc gttaggcgac
    16441 atgaccgcct ctgccagaaa ttcaagatca ttggaagcca gttctgctgc gatcactcgt
    16501 tgagaatctc tcagtgccac cttaattccc gtgacggatt cgggtgccat gttttcaaac
    16561 tgagcacacc aggcacgagc cctcgggagc acttcctcgg gggatgcact ttcatctacg
    16621 agaccaagag acagagcttc atccgatttc agagcgcgac cactgtacaa aagatcgcgc
    16681 gcggtcctga ttccaacaag ctgggtaagc acgtaagcag atgtagaagg aaagttgatt
    16741 ccgatgagag attctggaaa tccgattctt cccttcttct ccactagaat tctgtaatct
    16801 gaaaaaatcg caagcacagc gcccacgccc atagcatgcc cattcatagc gcaaacgact
    16861 ggtttttttg aaaagagcac tttgcctgac gcatccaggg cagattgtag aacagaatac
    16921 acgtcttcgc ggctgcgccc aatgaagata gaaggatcaa aaccattgga aaaaaacttt
    16981 tcatttctac ttgaaagcat cacagccttg atgcctctat ctacttctgc ttcttctatg
    17041 tttgcgccca gggccctgaa gaattcgaga cccatggaat ttgtatcatt ggtctggatg
    17101 taaagctcca ggatatgccc gtttctgact acgtcgatca tgtgactctc cgactaatcg
    17161 tctacgacta cctggtttct tcccgccgct ttcgcgcgat aaagcaatcg atccactctt
    17221 ccgaagaaat cttcaaaaga ctctccgcgt atatattcag tgcatccaat ggaaacagtc
    17281 gctggaatgg tcagatctct gtactgagta ggcgaatgtt caaaatcgac tcgaatcttc
    17341 tctgcagtct tgagtgctcc ctgaagagaa gtgtccggta agatcaccat gaactcttct
    17401 ccaccgcttc tgcccaccat gtctgttctg cgcgtgagcc ttctcagaag ctgagagaat
    17461 gtaataagaa ctgcgtctcc tgcctgatgg ccgtatgtgt cattcaccag cttgaaatga
    17521 tccagatcca gcacaagaat gctcaagctc gcgccgtatc tgcgacatct ttctatttcc
    17581 atatccagat gctcgtagac gactctgcga ttccagatgc cggttaacgg gtcactggtt
    17641 gccagctctc tcaactttct ttcgtaggtt gctttataaa gcgccgcgga caacacaggc
    17701 gtaagtgtca tgaggctttc gccgtccgta gattcaaagg gacgctctac tttgcgaaac
    17761 atgagaatag aaccaatgag ttcctgatcg gctagaatgg gggctgcgag catggtcatg
    17821 gcacccgcgc gaacgagtgc atcaattctc cgtggatctg cagagtattt tgcgatggtc
    17881 acaggcatct ttcgcgcgaa catttcttcg caaatgcttc cgctgaaggg tctggtgtat
    17941 ccgcgaaaca cttccgggaa tccgaggatg tgatcgtaag tgatttgctt gcgctcgtca
    18001 tcgagaagcc cgaggagtgc gagatccgca ttcatgagtt cgcacacacc gcgaagagct
    18061 gtgtccactg tctcttcggg gacagtctct gtgagtagat tcttgagtag attgttgaga
    18121 gagacggcag tttcgagtct gctctgtact ttgagctgtt gatttacgtc cgtgaccgtg
    18181 gtcacaacca ttctggattc gggcgctccg ggaacattga gtggactcga agacatcaat
    18241 acaaaacgct cgttgggatg atagcccagg cgcaaaactt ttccgaccac aggtagtccg
    18301 ctctgaagag ctgtttgaaa aggcgcttct tcgattggga tttcattccc gtccaggtcc
    18361 tgcgcctgcc agagagtcgc cgggtaaggt tctccgcgca gttctgcctg accgagagat
    18421 agaaggcctc ttgccgcttc attgattcga acaatgcgac catcctgccc catgagcata
    18481 atgccggacg gtaatatata gaagataccg cgaagaattt cctcacgttc tttcagagaa
    18541 cgattcaata aaattccagg cgtaatatcg cgaaagatca ccagggctcc ggaaacctgg
    18601 ccatgctgaa tcagaggcac gcctgtgatg attacatcca tagtagacgc tttgcgcgaa
    18661 agcaagatgg tctcatctgt ggagacaacg tctccctgca gaactggatc taaaattttc
    18721 tcaatggact gaggtggaat cagaaactcg tcgagtctac gacccatgag ttctgcggac
    18781 gaataaccaa gtagcccgcg cgcagactga ttggcgtaga tgatcatacc agatcgattg
    18841 aggccaatga ctccgtctgc gattgcattg agcaacagca gagtggtttc aacttcagac
    18901 gcagcaagca ggcccgaaat gcccgacccg gcttgctgga ctacttcgag aggaatgtga
    18961 acttcttcag ctcccctctg atcgctttgt tttgattcca tctttgggcg acgcctgtct
    19021 gaaaagtcgt cggaacttac agttcccatg aaaatttttg acagcgatgg aggcaagaaa
    19081 aagaaaatga caggcttgcc cgaactggcg agttcatccc atggcagaat cagcgaaaat
    19141 tggcatcatt ggcggcagcg gactctacgg actggaaggt cttcgcgtag aaacggaagt
    19201 aacgccggaa actccctggg gaaaaccatc ggacgcaatc gtaatcggcg aatatgcagg
    19261 caaacgagtc gcgttcctct ctcgccatgg ccgcggacat gtctacagcc cttcggaagt
    19321 gccctttcgc gcaaacatag ctgctcttaa aatgctcggg gtagagcaaa tcatcgcctt
    19381 ctcagccgtt ggatctcttc gcgaagaaat tgcaccccgc gattttgtcc tacctgtaca
    19441 gatcattgat cgcaccaaag ggattcgcgc atccacatat ttcgaaaacg gagtcgtggt
    19501 ccatgcaacc tttggagacc cgttcgatgg gcaactggcc actctggtga agcaggcagc
    19561 agcccgggtc aacggattaa aactgcatgc agacaaagtg gtcgtgtgca tggaaggtcc
    19621 cgcattttca actcgtgcag aatcacaact atatcgcagc attggcggag acatcatcaa
    19681 catgagtgtt ttgcctgagg caaaactcgc gcgcgaagct gaaattcctt accagatgat
    19741 ctgcatgtcc acagactacg atgcgtggaa agaacatgaa gaggcagtga ccaccgaact
    19801 tgtaatcggc catctcaacg caaacgcaga gagtgcgcga aaacttctgg cagaactcat
    19861 tccacttctt ggagagggag ccaatcctct caagggaagc atcaaaaatg cagtcatcac
    19921 ggcgccagaa aagagaaatc cggaacaact gaagaagctt gcgaaactat tgcccgggac
    19981 tttctgaatg attcgtactc ttgttgccgt atctgtgctg gtgatggctc ttcctctctc
    20041 agcgcaatta tccacggata cgcgccccac gaccggaagc acaccagaca cacccggtac
    20101 gtctaacaac tcgatccttc caggtgcagg caccgtgccc gcactgcagg aacgaagacc
    20161 cactccccgc gatccaagat atagacctcc tgtatccgtt cccggactca aggaaccatc
    20221 caatccggcg gctctctatt tgttctccgg agacgtggat gcgcaaaaat tcatggattc
    20281 gcgcaacaaa caaaagaaag caagcagata cgatgaagca gactacgacg acttgcctgc
    20341 tggcgggcgc gaaagagtct ggagagaaaa agaaatgctc agacaattcg gcgtggatca
    20401 ttttccatat gccgggcctg aacaggaaga accagaaagc gacctgagac gattccagat
    20461 tgttttcttt atttcacttc ccattacggc ggccgcttcc tacggcctgt ttcgcgctgc
    20521 caaaggccag agcgcgggtg ggttttcgag aggagaaacc atgggagtgt ttgctctggg
    20581 ccttggactt tcaggagcga tcggggcgta tgactactac caaaatcatc tcacgcaaag
    20641 aagtgccaat atagaactat tccacgctgc cgttacattc cgcttctaat cttttctact
    20701 ttccaaaacc aggcgggccc gtatgatcag gccgcgatgg atgagccaat aatctacgcg
    20761 ggctccactg cagtggacgc aagcgtacct gaagaattac ctctggtttt tctcatcgaa
    20821 ggggatccgg cacttcgctc ggacgtttcc actcagattc attacagcgg ttacagagtg
    20881 cttgcttttg aaaagataga gtccgcgctc gaagaatgca ggcgcaaagt tccggctgtc
    20941 atggttgtag atttgtctca gccagaaggc gatatggcca tggtgcttgc ggaaattcag
    21001 atgctcaatg agcattctat tcccatcata ttcttgtcgg atcgcggaga cattgaaact
    21061 cgagtgcgcg cggttcgatt tggtggaaac gcatactttg aaaaaccggc agatgcaggt
    21121 gcgctcattc gcgccatcga caataccgtt cacaaaaaag aaatcgagct ctatcgcatt
    21181 ctcattgttc aacaggaaac ggagactgcg gcaagatacg cagagatcct caattccgcg
    21241 ggaatgatga cggaaatact cacagatcca atgcggctgt tcttttctct gcaggatttc
    21301 aggccggact taatacttat gaatctttac tatccagatt gtctgggaat ggagatcgca
    21361 gcggtggttc gccaggaaga agcaaaccag agcattccaa tcatttatct tacggaagaa
    21421 actcaaatgc agaggattct gcctgcactc aacaaaggtg gagacgattt tctacctctt
    21481 cctgtgactc cggatcgttt gatttcttct gtggcggcca gggtggaccg ctcgcgcatt
    21541 ttacgttctc ttctggttcg cgatagtctg accggacttc tcaatcacac tgctgtgcgg
    21601 gagcagttag acgatctagt ccagcgcgca aaaaggaaga atgaaacgtt ctcttttggt
    21661 atggtggaca ttgacaagtt caagagagtc aatgatgaat acggtcaccc tgtcggggac
    21721 agagtcatac agagtctggc acgcaccatc cgccagagaa tacgcagggt agacgcggca
    21781 gggagatacg gcggagaaga atttgctgtc atctttgatg gtctggatac agacgcggct
    21841 gttcgcgcac tcgatcgaat tcgccgtgcc t
//
LOCUS       DAORFF010000003        21035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1003_length_21035_cov_3.666444, whole genome shotgun sequence.
ACCESSION   DAORFF010000003 DAORFF010000000
VERSION     DAORFF010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 21035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21035
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1003_length_21035_cov_3.666444"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PKK76_00245"
     CDS             <1..814
                     /locus_tag="PKK76_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HNL66918.1"
                     /translation="IRTTDKTVKTASASLNSGARIVITAKGAGMIEPNMATMLVYAFT
                     DAEIASADLDRMLRVVADRSFNRITVDSDTSTSDTFAILANGLSGVSISFPESASDAF
                     KNSSVFMPDVATVPGLDENAREFVTEFLALSQALARAIVQDGEGSTKILEVRITEARD
                     KTQALKIARSIANSPLIKTALHGGDPNWGRILMAVGKVFDEPVPFEQLKIYFGETLLR
                     YGDSSQLPELEKYLKQSSVVLRISLGAGQAEEKIWGCDLSREYISINADYTT"
     gene            811..2049
                     /locus_tag="PKK76_00250"
     CDS             811..2049
                     /locus_tag="PKK76_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNL66919.1"
                     /translation="MIWPPGRIASFKWKTFGFSLLSGAVVVLIFILISGAVPGDIPHY
                     NEVVTLLFLLSSVLFLFPARDRLHKILFGKNESALFGDAHHLDFIARQFTVESLTMEV
                     FPSLMQWMGVRAAKLAIAEGGHSHYVYYVYSEGQPVRTRQSYYHSLEELLLYAKQERR
                     PLAPELPVPEEIRKLLTRIRAAYFLPIFYRNRMVGFLILHDPPDNKYADRALETFAGK
                     AAASIQNSLLSSRFMDATAMDREFLVAEKIRKFLQRTEIPQIPGFKIEAFTGERAPIV
                     IEFLRAPDRSDVWFAVLFAADRLNGSTAILLSSRLGSLYSLSKKESSLNLHKLLTHFR
                     RNRADGFDRVDLCIAEIRPHENAILCMIDGPGFAVRNPLTPEKTIVSPGWRNVVDMKE
                     NSTLQIEYAGQPLLLFAAQK"
     gene            2046..4478
                     /locus_tag="PKK76_00255"
     CDS             2046..4478
                     /locus_tag="PKK76_00255"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL66920.1"
                     /translation="MNRIHYLVALTVLFVLYGIQFRETGFRLPIYYLPDGSIVSVRPA
                     DPPLRIQKILEIEGRVPHEAPATFRTDRAVLVYTDTGAAILKPEPVDRLQQFIDFAPI
                     ILLSALYLIAGIWFIQFGSDIYLSWFCVLASSFLYSFVAAIAGHGLDLLWQVFGFLLI
                     PAAFNLGLRTTGKEISGTLVIAEALVVLFFALIAYVGSDEVSTFGSLLLIQRGGFYVT
                     IVIVSGLQIENALRGSEDRIEKTKRWFLFSGTLIGLLLPAAAIEFRSGSGLDTLWFAG
                     ICASAFPVALVYGTYRIHVVPFQFVLGRSILAGLLTVFFVSLYAIVLLTHSLLLPNQD
                     QNYRWIVHLIFLLTLVFFLDPARQWMSAFIERNIWRLDSRLSESLKRIARIFSETSRI
                     DHAAEAFVDEIAATLTVRRCSLLISDRSFPGFRLKEDALIRMPDSSPLWEHIRPERLA
                     VAAYLAYAAGSRGILYRFLIESQFSMAAGVSGDEGPRFQAIIDKLLRRRRPVRKKGPY
                     VALLLGFRHDRSSFSLSEVRYIQEAARLARMLVSNFEILLAEVAKRRKMREIMLAGNT
                     QRSVTETWREADIRDLSIGAFSMPALSVTGDYVDLVPISPGRIAFFLGDVSGHGLGTG
                     YLVSAIRAMVRSHVQSGLGLSETMGTLNSFLMDRYRGNEFITLFAFYMDLEKGRLETL
                     NAAHPGPMVYRAATNTIETLRDTQRLPGLLPDPYHTSVLTMRRGDRLFLYSDGVTETF
                     SPASEPFGEARLRAFLYAHLGVNVSEIPDKLKRELTEHRQNPHCSDDTTFVAIEYAPR
                     QRGLFSFLNPDA"
     gene            4471..5316
                     /locus_tag="PKK76_00260"
     CDS             4471..5316
                     /locus_tag="PKK76_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNL66921.1"
                     /translation="MLDAHTIQIQRRALLEAEKLYAKPEFMASDPVELVHAYTDPLDQ
                     EVAALLCALFAYGNVKAMRGFLSALLSRMKTPSSILSKNFDPARIAGKLYYRFQSTRD
                     LRIILEGVSKIFRRVHKRAAGLESIFEGPTLRERISSFQKEFAHNVTLTRGVLHWIGS
                     PESTGAQKRFCMFLRWMTRTTFPDFGLYKTFHPRDLIVPLDVHMARMAQNLNWTTAAG
                     AGWKNAVEITRSLATIEPEDPLRFDFVITRPGILGVCRSILKPACDHCPVRRPCKVYG
                     QKILA"
     gene            5344..6018
                     /locus_tag="PKK76_00265"
     CDS             5344..6018
                     /locus_tag="PKK76_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNL66922.1"
                     /translation="MRVLCISAHPDDVEIGMGGTVASLTQKNIQVRILDLTNGEPTPM
                     GSPEIRAAESARAAEILKAERRTLDMPNRYLEDTIENRKKLASEIRDFRPEYMFAPYF
                     DDAHPDHIAASHLCDAARFYAKLTKSDIPGEPFFPRRIIYYFPVHIRLRVEPTFLLDI
                     SAHLETKKSAILCYQSQFVLSGKTHLIEHLMTENRYWGFQGGCDAAEPFFQKELPLIR
                     WPEGIA"
     gene            6015..7421
                     /locus_tag="PKK76_00270"
     CDS             6015..7421
                     /locus_tag="PKK76_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66923.1"
                     /translation="MIYFSDTKEQMRDKISRPAPRAPSLCSRPAASFDRSSILRTIPK
                     EKLNGIIILTGHQPTFHHPGILIKNVLAHNLARQSGGIAIHMVHDTDAEEIIFPYPER
                     VSDIVKKKSAKLNDASQILRHESFKPADRSAMLRLTDALRSEVRLVFGPEMIGAIHGG
                     LDLLEKSVREASRPMDVPDRLRLAWEAQNRIDVYTVYSSDLFESSSFQCFFEHVREHG
                     DRFREIYNRLLADYRRAKGIKNPAQPLPDLRPKELPFWVVQHGNRLPFIEGETSDGQI
                     LPRAVTLTLFCRLFFCDVFVHGLGGERYDRITDEILREFFDFAGAPFTAASATLALSS
                     RADLRIFSRSTQEVERDLRAVEFDPTRFLAKTDRVRREKEDLIAEWHKNPASRSRLHR
                     EFVRLNEQARHGLNGVIENLEWEKNLARVANANKTFFGARDLPYFFYDLSEIFKTVND
                     YVFFEKRSPSASLAEARS"
     gene            complement(7364..7702)
                     /locus_tag="PKK76_00275"
     CDS             complement(7364..7702)
                     /locus_tag="PKK76_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66924.1"
                     /translation="MQEKQKQPSKFMRVYKEFEPEEVKRHLLLIDDLYGTCANCGQLG
                     LNFTKDKVCPGCKTTFSFLMTKLKNPGDVIKILNRIKTDSLPFTMIEKDDYDRASARD
                     ALGDLFSKKT"
     gene            complement(7747..8823)
                     /locus_tag="PKK76_00280"
     CDS             complement(7747..8823)
                     /locus_tag="PKK76_00280"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNL66925.1"
                     /translation="MQELRHLSLLYDSIIKPALFRIDPESAHELVAGTVSFVTHSPIG
                     AAVVRSVLGYSSTRLAVTLAGIDFENPLGMAAGFDKTGRLYPFLSCAGFGSVECGTFT
                     RHAQNGNPKPRLFRFPEQQALVNRMGFNNPGAEEAARIIATQKHGAVRGINLGKSKIT
                     DLDGAAEDYLYSLRALVGFADYVAINVSSPNTPDLRKLQEKKRLSSLLRALRKECAPP
                     LILKIAPDLTTEEIEDVLDASSGIIDALILCNTTIDKSSVPGAESLEGGLSGKPLAAR
                     SLALLRQVRAMTGGQLPLISAGGIDSAESAIERIRAGASLIQIYTSYIYGGPDLPRRI
                     LSGIDRYLEKNSMRMPDLIGADAR"
     gene            complement(8802..9422)
                     /gene="lexA"
                     /locus_tag="PKK76_00285"
     CDS             complement(8802..9422)
                     /gene="lexA"
                     /locus_tag="PKK76_00285"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNL66926.1"
                     /translation="MRELTEKQNAILGFIQDYIREQGYPPTIREIARRFKITAKGAYD
                     HMKALEKKGYIKTEKNRSRAMEILKSADGSAPVHPADTVNVPLVGRVAAGLPILAEEN
                     VDEYLSFPRSMLPDAGIFALRVAGESMKDAGIYDGDIAVVKKCDTAENGEIVVALLED
                     EATLKYFYKDKKKVKLVPANSAFRPILTDTVTIVGKLVGIYRSYGI"
     gene            9513..10679
                     /locus_tag="PKK76_00290"
     CDS             9513..10679
                     /locus_tag="PKK76_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNL66927.1"
                     /translation="MSVKKTARNSQSCGVPGQPENRPGGPVETLARLLIVAAYGALAY
                     LLWGMLAGILFPVLAALLLAYLLSPLITWMGKKGISRGLAIALVFVGFLLLFFSTVVV
                     LYPVIAREVVTVVEKFPGLIDLIENKTIPWLRDTAGVDVPTTVSGAMENYGESLKQAF
                     PGMLNKAGTWFVAAATSTGSILSGILNVVLIPVFTFYFLADFQIIVSFFENLIPDHRR
                     ERTVAALSRVDAAVGNWLRGQIEVAIVLSLLYGTGLAIVFGIFGLGAKTGFAIGALTG
                     VLNIIPYVGVGTGLVLSLAITLLEWQGIGAVISTASVFVGVQIMDGYFITPRIVGEKV
                     GLGPAAVIISLLVGGGLFGLAGMLLAIPVAAALRTLWPDILEKYKSSRFYTGSP"
     gene            complement(10682..11083)
                     /locus_tag="PKK76_00295"
                     /pseudo
     CDS             complement(10682..11083)
                     /locus_tag="PKK76_00295"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
     gene            complement(11123..11929)
                     /gene="murI"
                     /locus_tag="PKK76_00300"
     CDS             complement(11123..11929)
                     /gene="murI"
                     /locus_tag="PKK76_00300"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNL66928.1"
                     /translation="MADSRPIGVFDSGMGGLTVLSELVRRLPNERFIYLGDTARVPYG
                     SRSPRTVLRYSLEIAAFLGQSDIKMLVVACNTATAHALPELRARMSIPVIGVIDPGVR
                     ALLSRTKSRKVGVIGTRSTIKSGAYETAIRGIDPGMDVTSLACPLFVPIVEEGWMNKD
                     FARLAVREYLTALRDHGVDAVVLGCTHYPLLRRAIESEFPSFTLVDSSVEIADAVTAM
                     IQERDAASAGPGSVRILVTDVTEQVSDLKRLFFPHPVETIEEVVVPELHV"
     gene            complement(11922..13145)
                     /locus_tag="PKK76_00305"
     CDS             complement(11922..13145)
                     /locus_tag="PKK76_00305"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNL66929.1"
                     /translation="MLIVQKFGGTSVGDTDRIKNVANRIKGYYEQGHRLIVVVSAMGH
                     TTDELIDMAMKLNGNPPRREMDMLLSTGEQISMALMAMALEAIEVPAISFTGGQLQIM
                     TDGTHSNARIRGLDTTRLDEAFAQKKVCIVAGFQGVNENQEIMTLGRGGSDTTAVALA
                     VAVKADECEIYTDVDGVYTTDPNKVPKAKKLKQICYEEMLELASMGAGVLHNRSVELA
                     SKFGIVVHVRSSFNHNPGTLVVPEEKIMDMEKVLVRGVSLKSDQARVAALDIPDRPGL
                     AAGLFEKLATASINVDMIVQSSGHDGKNTISFTVPSNDLANARGILEKFLAELGGGRI
                     AVEENIAIVSAVGVGMKSHSGVAATMFQALAKENINIEMISTSEIKISVVVQKDQAKR
                     ALQAAHTAFGLDQNG"
     gene            13271..14203
                     /locus_tag="PKK76_00310"
     CDS             13271..14203
                     /locus_tag="PKK76_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNL66930.1"
                     /translation="MKQSSKSHRQKATKPVDTLQRLLGRRATTVRYQSGFLVRGRLHP
                     FLDLAKDQGDAGWSNDIANDLEEHSQHHFIDVYNRRLAVEGLRPALSIKGAAFLDAGC
                     SSGYLLADVQAAFPDASLFGADFFPAGLAHCHRAHPEIPLFRMDLTACPFPDNSFDAL
                     SCLNVLEHIQDDLAAARTLFRILKPAGLAAITVPAAPHLYDLFDEIHFHERRYSLLQT
                     QDLLRAAGFKIRFANYFGSLIYPAFYAVKRRNQNRYGALSFEQKRDLAMSQIQKTSQS
                     RIMNAVCRFEEAIGKRLTYPFGIRACVIAEKPPG"
     gene            complement(14226..15095)
                     /locus_tag="PKK76_00315"
     CDS             complement(14226..15095)
                     /locus_tag="PKK76_00315"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNL66931.1"
                     /translation="MKTILITGASGFIGSRACSFFRNAGYLVRAHSRSPIPDQDVVVG
                     DLQTEDAIEGACKDVDGIFHIAGLAHSRGVDEGALERVNVRWTRELANAAKRRSVPFL
                     FISSSKVYGETGHFSEASPLNPQDAYSRAKVRAEDEIQKATDRFVILRPPPVYGVGSK
                     GSLRFLIRAVQKGWPLPVGSIRSLRSYVFVDNLLSAAEMFLRSERSGIWNVTDDEDVS
                     LADLAHRIARASRSSDFSFRFPLFPLRVMGILRPGMYDKVAGEFTLDIGRLKACGYEP
                     SFSVNEGLRIACS"
     gene            complement(15198..15974)
                     /locus_tag="PKK76_00320"
     CDS             complement(15198..15974)
                     /locus_tag="PKK76_00320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNL66932.1"
                     /translation="MNKPSVSIVTVCFNSEATIRDTVRSVMNQTHPVEYIVVDGASQD
                     GTVDVVRELLSQSTLNYQIISERDRGMYDAMRKGIAASHGDVVAILNADDRFESNESV
                     EKVASAFAPDIDVVIGDVIITDQDFGKVLRYYSASAFRPEDLTRGIMPPHAGFYARRH
                     LFEKLGSYTPDYRYAGDFELILRFLLKARVKFRYLPEILVRMRTGGLSNRSLRHRFNL
                     TKEVLRALKENGYPSSWLLVLSKIPGKLFQYFRRPPGQAR"
     gene            complement(15971..16957)
                     /locus_tag="PKK76_00325"
     CDS             complement(15971..16957)
                     /locus_tag="PKK76_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNL66933.1"
                     /translation="MHLILDSIIYQLQAHGGITQFWQNLCSRMKSYPDLDTEEIAGHK
                     LLRYNPLWMRCDVFHSSYYRLNFWPGVRNVVTVYDFLYERGHVESRFRHLHIWQKRAA
                     IRRADAVVCISENTRQDLLRFYPDAENRASVVHLAAGHSEPGAPGRATAHIETRPFFL
                     FVGHRYGYKQFELVVQAMTEARLFDQYDLVCTGSPFSEAEQKSFSDNQIATYVKSIPG
                     ATPGEMQALYAHAKALVYPSLYEGFGLPVVEAMTAGCPVVACDSSSIPEVAGNAALLF
                     LPGDLPGLVRALEQVCQKGARERYAALGQIQAEKFSWEKTVAGYHKIYQALK"
     gene            complement(16957..17733)
                     /locus_tag="PKK76_00330"
     CDS             complement(16957..17733)
                     /locus_tag="PKK76_00330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL66934.1"
                     /translation="MNPVCRVCGEATHSFGEALLLAKHRVHYFECDVCGYVQTEDPYW
                     LEEAYQNPVNLTDTGLLGRNVMLSRMTLLLLRRYFKKGDFVLDYSGGYGVLTRLLRDA
                     GAECYWHDPYTPNVFARGFEMHRAARSFSLVTSFESFEHFVHPLEEIRKISQISSEIF
                     FSTRLLPPHRPQPGQWWYYGLEHGQHIGFFTPASLAHIAKELGLHVVSAFGAYHFLSR
                     RKIHPLSFAVTLAFSRTPFVSLFHPGPSLTVRDSEFLSRI"
     gene            complement(17730..18623)
                     /locus_tag="PKK76_00335"
     CDS             complement(17730..18623)
                     /locus_tag="PKK76_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNL66935.1"
                     /translation="MSLETPVVFFAFNRPDVTRRVFERIAAQKPARLFLIADGARNAT
                     ESKLCEEVRSIITNVNWSCDVQTNFATENMGCRARLSSGISWALERSEEVIILEDDCL
                     PHPDFFRFCSELLARYRTDERVFSISGDNFQPESRGYSYYFSRYPHVWGWATWRRAWQ
                     KYDVDLASWPELSRNHSFRELVKGPAGRYWARNFDLICKGFNTWDIQWTFACLLNQGL
                     TILPDKNLVSNIGFGAEATHTKRKSPLEALGTTELDWPLRHPPFVARNEAADEFTEHR
                     VFRVRPVDRLIYLARQLWRYL"
     gene            complement(18620..19456)
                     /locus_tag="PKK76_00340"
     CDS             complement(18620..19456)
                     /locus_tag="PKK76_00340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNL66936.1"
                     /translation="MVHIILPVHNRIELTRAFVESLVQSSSKDFHIVLVDDGSTDGTS
                     EMVCESFPFTTVIRGTGNWWWGGSLHQGYLWARSAELPTSDVIMTVNDDTALDPAFLE
                     NAERLLKERPRDLLVATARDGRDILQEQRMKVDWLRLRFRPIRDGETPDWCTTRGLCM
                     RVSEFLSIGGFYPTLLPHYLSDVEYTLRARRKGHPIFSDPSVSLRMNTESTGHHLLKS
                     MRFWPYVKTVFSRKTSHNPIYMTTFLFLAAPFWAIPTGLLKVWALPLVEFFGFRREGE
                     IA"
     gene            complement(19473..20111)
                     /locus_tag="PKK76_00345"
     CDS             complement(19473..20111)
                     /locus_tag="PKK76_00345"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNL66937.1"
                     /translation="MIGKLKSLILRAIDAAGYTISRKPRWKVSAEKRILKVESRKIHY
                     GSGGRLMSGWVNVDLYPSESLIADPVRVSANLTLEHPFADGWFLYAFSEDFFEHLEQK
                     QQLLFLVEAYRVLAPGGVLRMSFPGLEGVLMRHFSDNRLASYGQGVYDSYDVFEHKHF
                     ASRAELELMCRHIGFSSVDFEIKYGESRHEPLKGLDSRVEQIGLNTYAEITK"
     gene            complement(20126..>21035)
                     /locus_tag="PKK76_00350"
     CDS             complement(20126..>21035)
                     /locus_tag="PKK76_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66938.1"
                     /translation="KIEPNDLNGLLKTSAVDWWKIYQAFFLGRDLIDFDPVLLQKSAR
                     EIFSLLPAKSIFFGISDFVSGLFGFYFVTPTEHAVRIWKFTARLVLSALCIVLSAGFV
                     YSVTHFYERRRKNLDSTAIPAVAVSVFISAAACIVLFLVLRQSYWAAGKALSYFSPFF
                     VSLLAWSAFEKTRPLFYAASVIFITLHVWAGAGRIPAAAHKSGIHFAYPYPVADPQIK
                     ASHNWDFHTFDEDVKSCHVVRIESPEIWFGRYAMLYLTSRQKPFVTDMPVDTLFGFAK
                     TKMGVMPEKRHDCLLRESGIRPVVER"
BASE COUNT         5280 a         5517 c         5164 g         5074 t
ORIGIN      
        1 aattcgcaca acagacaaaa cagtaaagac tgcaagcgct tccctgaact caggagcgcg
       61 cattgtaatc acggccaagg gagcagggat gattgagcca aacatggcta ccatgcttgt
      121 ctatgcgttc acagatgcag aaattgcttc cgcagatctg gacagaatgc tgcgtgtagt
      181 cgcagatcgt tcctttaatc gcataacggt agattcagat acgtccacgt cagatacatt
      241 tgcgattctt gcaaatggat tgtccggcgt ttcgatttcg tttccggagt ctgcctcaga
      301 tgcattcaaa aactcatcag tcttcatgcc ggacgtcgca accgttcctg gactggatga
      361 aaacgcgcga gaattcgtaa ccgagttcct tgcactttcg caggcactgg cccgcgcgat
      421 tgttcaggac ggagaaggat ccacaaaaat cctggaagtg cgaatcacag aagcaagaga
      481 caaaacccag gcgctcaaga tcgctcgttc cattgcaaac agcccgctga tcaaaacagc
      541 tttgcatggt ggagatccaa actggggaag aattctcatg gctgtgggga aggtattcga
      601 tgaaccagtt ccatttgaac agctgaagat ttatttcggg gagacattgt tgcgctatgg
      661 cgattcttcg cagttgcctg agcttgaaaa atacctgaag cagagctctg tggtgctgcg
      721 gatctccctg ggtgcaggcc aggcagaaga aaaaatctgg ggctgtgatc tttctagaga
      781 atacatcagc atcaacgcag actacacaac atgatctggc cgccggggcg catcgcttcg
      841 tttaaatgga agactttcgg gttttccttg ctctccggag cggtcgtagt tctgattttc
      901 attctgatct caggagctgt tcctggggac attcctcact acaatgaagt ggtcactcta
      961 ttgtttctgc tttcctccgt tctctttcta ttccctgcca gagatcgact gcacaaaatc
     1021 ttgtttggca aaaacgaatc cgctctcttc ggagacgcgc atcatctgga tttcatcgcg
     1081 cggcagttca ccgtagaatc actgaccatg gaagtatttc cgtctctcat gcagtggatg
     1141 ggagtgcggg ctgcaaaact cgccatcgca gagggtggac acagccatta tgtgtactac
     1201 gtatattcag aaggtcagcc cgttcgaacg cgccagtcgt actatcattc gctcgaagag
     1261 cttttgctct acgcaaaaca ggaacgcaga ccacttgcac ccgagcttcc agtgccagaa
     1321 gaaattcgaa agctactcac tcggattcgc gcagcttact ttcttcctat cttctataga
     1381 aatagaatgg taggattctt gattcttcac gatccaccag acaacaagta cgcggataga
     1441 gcactcgaaa catttgcagg gaaagccgca gcctccatcc agaactctct gctctcctcc
     1501 aggttcatgg acgcaactgc gatggaccgg gaattcctgg ttgctgaaaa aattcgaaag
     1561 ttccttcagc gcacggaaat accacagatc ccgggcttca agatagaagc attcacagga
     1621 gaaagagcgc ctatcgtcat tgagttccta cgcgcgccag atagaagtga tgtatggttc
     1681 gccgtgctgt ttgcggcaga cagactgaac ggatccaccg caattctgct ctcaagtcgg
     1741 cttggcagcc tgtattccct ctccaagaaa gagtccagcc tcaatctgca caaactgctg
     1801 actcacttcc gtcgaaatcg cgcggacgga tttgatcgag tggatctttg catcgcagaa
     1861 atcagaccgc atgaaaatgc aatcctctgc atgattgacg gccccggatt cgctgtgcga
     1921 aaccctctga ccccggaaaa gaccattgtt tcccctggat ggagaaacgt ggtagatatg
     1981 aaggaaaact caacccttca aattgaatac gcaggacagc cattgctcct gtttgcggcg
     2041 cagaaatgaa cagaatccac tacctggtcg cgctcaccgt tctatttgtt ctctacggaa
     2101 ttcaatttcg cgaaacagga tttcgcctgc ccatctacta tttgccagat ggatccatcg
     2161 tttctgttcg tcctgcggat cctcctcttc gcatccaaaa aatccttgag atagaaggta
     2221 gagtgccgca cgaagctccg gccacttttc gcacagacag agcagtgctt gtatatacgg
     2281 acacaggcgc tgccatactc aagccagaac ctgtcgatcg gctgcagcaa ttcatagatt
     2341 ttgcgcccat catcttactc tcagcgctct atctcattgc aggaatctgg ttcattcaat
     2401 ttggaagcga catctatctt tcctggttct gcgttctggc atcttccttc ctctattcat
     2461 ttgtggcggc catcgccggc catggactgg atctactctg gcaggtgttt ggatttttac
     2521 tcatccccgc cgcattcaac ctgggacttc gcacgactgg aaaggaaatc agtggaacgc
     2581 tcgtgatcgc agaggctctg gtcgttctat tctttgcttt gattgcctat gttggatcag
     2641 atgaagtgag cacgtttggg agtctccttc tcattcagag aggtggattc tatgttacga
     2701 ttgtcatcgt ctctggcctg caaatagaaa acgcactgcg cggctcggaa gacagaattg
     2761 agaaaacaaa aagatggttt ttgttttctg gaacgctcat tggacttcta cttccggctg
     2821 ctgccattga gtttagatcc gggtctggtc tcgatactct atggtttgca ggcatttgcg
     2881 cctctgcatt cccggtggca cttgtatacg gcacctatcg cattcatgtg gtgcctttcc
     2941 agtttgtctt gggacgttcc attcttgcgg gactgctgac tgtttttttt gtttctcttt
     3001 atgcgatcgt acttctcact cactcccttc ttttacccaa ccaggatcaa aactacagat
     3061 ggattgttca tttgatcttc cttctgaccc tagtcttttt cctggacccc gcgcggcagt
     3121 ggatgagcgc attcatagaa agaaacatct ggagactgga ctctcgcctt tcagaatcac
     3181 tcaagagaat cgcaagaatc ttctctgaga caagtcgcat tgaccatgcc gcggaagcgt
     3241 ttgtagatga aatcgcggca acactcaccg ttagacgctg ttctttgttg atatccgata
     3301 gatcctttcc cggtttccga ttaaaagaag acgcactcat acgaatgcca gactcaagcc
     3361 cgctctggga acacattcga ccggaacgtc tggcggtcgc cgcgtatctt gcttacgccg
     3421 ctggaagtcg aggaatactc tatcgattcc tgatagaatc gcaattctct atggcggccg
     3481 gagtttctgg agacgaaggg cctcgcttcc aggcgatcat agacaaacta ctgcgcaggc
     3541 gaagacctgt gcgaaagaag gggccatatg ttgctctatt gctgggcttc cggcacgatc
     3601 gatccagctt cagcctgtct gaagtgcgat acatccagga agcggccaga cttgcgcgca
     3661 tgctcgtgtc taactttgag atcctcctcg cagaagttgc aaaaagaagg aagatgcgcg
     3721 agatcatgct ggcaggaaac acgcagagaa gcgtaacgga aacctggcgc gaagcagaca
     3781 tccgtgattt gagcattggc gcattcagca tgccagcgct ttccgtcacc ggagactacg
     3841 tggacctggt gccgatttcg ccgggcagaa tcgcattctt tctgggcgat gtaagcggcc
     3901 acggccttgg aactggatac ctggtaagtg ccatccgcgc catggtaaga tcgcacgtgc
     3961 agagtggtct tggtctttct gaaaccatgg gaactctcaa cagcttcctt atggatcgat
     4021 accgtggaaa tgaattcata acgttattcg ccttttatat ggatctggaa aaaggaagac
     4081 tggaaacttt gaatgctgcg catccgggcc ccatggtgta tcgcgcggct acaaacacga
     4141 ttgaaacact tagagatacg caacgccttc cggggctact accggatccg tatcacactt
     4201 ctgtattgac catgagacgg ggagaccgct tatttttgta ttcagatgga gtgacagaaa
     4261 cattcagccc tgcatctgaa ccattcgggg aagcgaggct tcgcgctttt ctctacgcac
     4321 atcttggtgt aaatgtaagt gagatcccgg acaaactcaa aagagagctc acagagcaca
     4381 gacagaatcc acactgcagc gacgacacta cgttcgtggc aatagaatac gcacctcgcc
     4441 agagaggcct tttctctttt ctgaatccgg atgcttgacg cacacacgat tcagattcaa
     4501 aggcgcgccc tcctggaagc ggagaaactg tacgcgaaac ctgaattcat ggcaagtgat
     4561 ccagttgagt tagttcacgc ctacacagat ccgctggatc aagaagttgc agccctactt
     4621 tgcgctttgt tcgcctatgg aaacgtaaaa gcaatgcgag gttttctgtc cgctcttctt
     4681 tcccggatga aaacaccatc ttccatcctc agcaagaact ttgatcccgc gcgcattgca
     4741 ggaaagctct actacagatt tcagagcacc cgggatcttc gcatcattct agaaggagtt
     4801 tcgaaaatat tcagacgcgt ccataaacgt gccgccggcc tcgagtctat ctttgaggga
     4861 cccactcttc gagagaggat ctcttctttt cagaaagagt ttgcgcataa cgttacactg
     4921 acgcgtggag tgctacactg gatcggatcg ccggagagca caggagcgca aaagagattt
     4981 tgcatgttcc tgcgatggat gacacgaacc acattccccg attttggact gtacaaaaca
     5041 tttcatccgc gagatctcat tgtgccactc gatgtgcata tggccagaat ggcccagaat
     5101 ctaaactgga ccacggcggc cggcgccggc tggaagaacg ctgtggaaat cacacggagt
     5161 ctggcgacca ttgagccgga agatccgctt cgtttcgatt ttgtgatcac tcgacctgga
     5221 attctgggcg tttgcagatc catactgaag cctgcctgtg accactgccc ggtccggcgg
     5281 ccatgcaagg tatacgggca aaaaatcctc gcctgagtgt agttttgcga atttcatgct
     5341 ctggtgcgcg tcctttgcat ctcagctcac ccggacgatg tcgaaattgg catgggtggc
     5401 actgttgcgt ctctcaccca aaagaacatt caggtgcgca tcctggatct aacgaacgga
     5461 gagcccacgc ccatgggtag tccggaaatt cgggcagcgg aatctgcgcg tgcagcagaa
     5521 attctcaaag cagaaagacg cactctggat atgcccaacc gctatctgga agataccatc
     5581 gaaaacagaa agaaacttgc ttctgaaata cgtgactttc gtccggagta tatgtttgcg
     5641 ccgtacttcg atgacgcgca tccggatcat atcgccgcaa gccatctctg cgacgcggcg
     5701 cgattctatg cgaagctaac aaagtccgac attcctggag agccgttttt tccaaggcgc
     5761 atcatctatt attttccggt tcacattcgc ctgcgcgtgg aacccacgtt tcttctggat
     5821 ataagcgcgc acctggaaac aaagaaatct gcgattctct gttaccagtc gcaattcgtt
     5881 ctcagcggca agactcatct catagagcat ctgatgacgg agaatcggta ctggggattc
     5941 cagggtggat gtgatgcggc agagcccttt tttcaaaagg agcttccatt gattcgctgg
     6001 ccagagggaa tcgcatgatt tacttctccg acacaaagga gcaaatgcgc gataagatct
     6061 cgcggcccgc tccgcgtgcg cccagtctct gtagcagacc ggccgcgagc ttcgatcgat
     6121 cttctattct tcgcaccatc ccaaaagaaa agctaaacgg catcatcatt ctcacgggac
     6181 accaaccgac atttcatcat cctggaattc taatcaaaaa cgtgctggca cacaatctgg
     6241 ccagacagag cggcggaatc gccattcaca tggtgcatga cacggacgca gaagaaatca
     6301 tctttcccta ccccgaaaga gtgtcagaca tagtaaagaa gaagtcagcc aaactcaatg
     6361 atgcttctca aatactcagg catgagtcat tcaaacccgc ggacagatct gcgatgcttc
     6421 gcctaacaga tgctttgcga tcggaagtgc ggctcgtgtt tggcccggag atgatcggag
     6481 cgattcacgg aggactggat ttgctcgaaa aatccgtgcg ggaagcaagc cggcccatgg
     6541 atgtgccgga tcgtctcagg cttgcctggg aagcgcaaaa ccgcatagac gtgtacaccg
     6601 tatactcaag cgatctattt gagagttctt ccttccagtg cttctttgaa cacgtgcgcg
     6661 aacacggaga tagattcaga gaaatataca atcgccttct ggcggactat cgtcgtgcaa
     6721 agggaatcaa gaatccagcc cagccactgc cagaccttcg ccctaaagaa cttcctttct
     6781 gggtagtcca gcacggaaat cgcctgccgt tcatagaagg ggaaacgtca gacggacaga
     6841 ttttgccgcg cgctgtcact ctcactctat tctgtcgtct cttcttctgc gatgtatttg
     6901 tgcacggact tggtggagaa agatacgatc gcataacgga tgaaatcctt cgcgaatttt
     6961 ttgattttgc aggagcgcct ttcactgctg cgtctgctac acttgctctc tcctcgcgcg
     7021 cggatctccg catctttagc agatccaccc aggaagtgga acgcgatcta agagcagtag
     7081 aattcgatcc cacaaggttc ctcgcaaaaa cagatcgcgt tcggcgggaa aaagaggatc
     7141 tcattgcaga gtggcacaag aacccggctt cgcgctctcg tctgcacagg gaatttgtaa
     7201 ggctcaacga acaggcaaga cacggactga acggagtgat cgagaatcta gaatgggaaa
     7261 agaatctggc gcgcgttgca aacgcaaaca aaacgttctt tggagcgcga gaccttccgt
     7321 atttcttcta tgacctgagc gaaatcttca agactgtaaa cgattacgtt ttctttgaga
     7381 aaagatctcc cagcgcgtcc cttgcggaag cacgatcgta gtcgtctttt tcaatcattg
     7441 taaaaggcag agaatccgtc ttgattcgat tgagtatctt gatcacgtcc ccggggttct
     7501 tcaatttagt catcagaaaa gagaaagtgg ttttgcagcc agggcagact ttgtctttcg
     7561 taaaattcaa tcccagctgt ccgcagttcg cgcaggttcc gtacagatcg tctatgagaa
     7621 gcaggtgacg cttcacttcc tctggctcga attccttgta cacgcgcatg aactttgagg
     7681 gctgcttttg cttctcttgc atgctatgtc cgtggcccgc acgtcttttg aacgcaagac
     7741 aaattttcag cgagcgtctg cgccgatcag gtccggcata cgcatgctgt tcttttccag
     7801 gtagcgatcg attccggata gaatgcgccg cgggagatct ggtccgccgt aaatgtagga
     7861 agtgtaaatc tggatcaggc ttgcgccggc ccggattcgt tcaatggcgg actcagcaga
     7921 atcaattccg ccggcagata taagaggaag ctggccaccg gtcatagctc gcacctgacg
     7981 aagaagtgca agacttcgcg cagcaagcgg tttaccagat aatcctccct ccagggattc
     8041 agcacccgga acagaagatt tatcaatcgt agtgttgcac aggatgagcg catctatgat
     8101 gccactcgaa gcatccagga catcctcaat ttcttctgta gtgagatctg gcgcgatctt
     8161 tagaatcaag ggaggcgcgc attcctttct aagcgcgcga agaagagagg agagtctttt
     8221 cttttcctgc agcttgcgaa gatccggagt gttgggagag ctcacattga ttgcgacgta
     8281 gtcagcaaat ccaaccagag cgcgaagaga atacaaataa tcttctgccg caccgtccag
     8341 atccgtaatc ttgcttttac caagattgat tccgcgcaca gctccatgtt tttgagtggc
     8401 gatgatccgg gctgcttcct ccgctcctgg attgttgaac cccattcgat ttacgagagc
     8461 ctgttgttcc ggaaaacgaa acagtcttgg ctttggattt ccattctgag cgtggcgcgt
     8521 gaatgttccg cattctacag agccaaagcc agcgcaggat agaaatgggt agaggcgacc
     8581 cgtcttatca aagccggctg ccattccgag agggttttca aaatcgatgc ctgcgagagt
     8641 gacggcaagc ctggtggatg aatagccaag aacgctgcga acgactgcgg cgccgatagg
     8701 agaatgtgtt acgaacgaaa cagtacctgc caccagctca tgcgcggatt caggatcgat
     8761 ccgaaacaga gctggtttga tgatggagtc gtaaagtaaa ctcagatgcc gtagctcctg
     8821 tatatgccga ccagttttcc aacgatggtc acagtgtctg tcaggattgg gcgaaaggca
     8881 gagtttgccg gaaccagttt cactttcttt ttatctttat agaagtattt gagagttgct
     8941 tcatcttcca gaagtgccac cacgatttcc ccgttttctg cagtgtcgca tttcttaacg
     9001 actgcgatgt ctccgtcata gatgccagcg tccttcatgg attctccggc cacgcgaaga
     9061 gcgaaaattc ctgcatccgg aagcatggaa cgaggaaagc tgaggtattc atctacattc
     9121 tcttccgcaa ggataggcaa gcctgcggcc acgcggccca ccagaggcac attgactgtg
     9181 tctgccggat gcacaggtgc acttccatcc gcgctcttta gaatctccat ggcgcggctt
     9241 cgattctttt ctgtcttgat gtagcccttt ttttcaagtg ctttcatatg atcataggcg
     9301 cctttggccg tgatcttgaa acgccttgcg atttcacgaa tggtgggcgg gtaaccctgc
     9361 tcgcgaatgt agtcctgaat gaagcccagg atggcatttt gcttttccgt caactctctc
     9421 atgtttgcta tacaaacgac acgcaaaaac ctgtcaagca aaaccgaaat cctccgaatt
     9481 gactccagca ccagcgcccc cagccttgcg ggatgagcgt caaaaaaaca gccagaaaca
     9541 gccagtcctg tggcgtccct ggccagccgg agaatcgtcc aggtgggcct gtggagaccc
     9601 tggctcgact tctgatcgtg gctgcatatg gtgcacttgc gtatctgctg tggggcatgc
     9661 tggcggggat tctgtttcca gttctggccg ctttgcttct tgcgtatttg ctttctccgc
     9721 tcatcacatg gatgggtaag aaagggattt cgagaggact tgccatcgcg cttgtttttg
     9781 tcggattctt gcttcttttc ttttccacgg ttgtggttct ttatcccgtc atcgcaagag
     9841 aagtggtcac agtcgttgaa aagtttcctg gtctcattga tcttatagaa aataaaacca
     9901 tcccgtggct cagagatacg gccggagtgg atgtgcctac tactgtctcc ggggcgatgg
     9961 aaaattacgg cgaaagtcta aagcaggcat tcccaggaat gctcaacaaa gcaggcacct
    10021 ggtttgtagc tgcggccact tctaccggaa gcattctatc tggaatcctg aacgttgttt
    10081 tgattccagt cttcacgttt tactttctcg ctgattttca aatcattgta tccttcttcg
    10141 agaatctcat tccggatcac aggcgcgaac gaaccgtcgc tgcgctctcg cgcgtagacg
    10201 cagctgtagg caactggctt cgcggacaaa ttgaagtggc tatcgtctta tcgttgttat
    10261 acggaacggg ccttgccata gtatttggaa tctttggcct gggtgcaaag actggatttg
    10321 ccatcggagc gcttaccggt gttctcaaca tcatccctta cgtcggagtc ggcactggcc
    10381 ttgtcctttc tcttgcgatc actctactcg aatggcaggg aataggcgct gttatctcta
    10441 ctgcttctgt attcgtgggc gttcagatca tggatggata cttcatcact ccacgcattg
    10501 taggagaaaa ggttggtctg ggaccagcag cagtcattat atccctgctg gtaggcggag
    10561 gattgttcgg tctggctgga atgcttcttg cgattcctgt agcggcggcg cttcgcacac
    10621 tgtggccgga tatccttgag aaatacaaat ccagcagatt ttacaccggg tctccgtagt
    10681 tttaaggaga gaaaaagcga tggatgttga acccaactgt ccaatcacgc ttacgataat
    10741 tgtaggccgc accgcgaagg agagtcgtat aggcaaggtc tcgtgaattt gtaacaatga
    10801 gttggaagat atgtccaccg gtatcgatat tcactccgat gctcgctgga atgcttgcat
    10861 atcgtaccgg ctcatcctga accacattga ccacataagc atagagtaaa ccagcagggg
    10921 aaaggtacct tgcattgatg tccgatgctg taagctcggt tccatattta gaacgttgat
    10981 cctcaagtgc atagttggat gccagatagt cgcgatgcgc cgtgagcatg gtttccagca
    11041 taatgtcgat tcgcttcgtg atcttaatgc ggccaccaag gtcaacgccc ggaacctggc
    11101 ttcgccaggg tgagtcccag gttcagacgt gaagctccgg tacaacaacc tcctcgattg
    11161 tttctaccgg atgaggaaaa aataaacgtt tcagatcaga gacctgctct gtaacgtctg
    11221 taactagaat tcgaacactg cctggccccg ccgaagccgc gtctcgctcc tggatcattg
    11281 cagtcaccgc gtctgcgatt tcaacagacg aatctactag agtgaaagaa ggaaattcgc
    11341 tttcgatagc gcgtctgagg agcggataat gagtgcaacc aagaacgacc gcatctacac
    11401 cgtgatcccg gagtgcagtc agatattcgc gcaccgccag tcgtgcgaaa tctttattca
    11461 tccagccttc ttctacgatg ggaacaaaga gaggacaggc aaggctcgtt acgtccattc
    11521 caggatcgat tccgcgaatc gctgtttcgt aggcgccaga tttgatggta gatctggtcc
    11581 caatgactcc tacttttctg ctcttcgtgc gagacaagag agcgcgcacc cctggatcaa
    11641 tcacgccgat gactggaatg gacattctgg cgcgaagctc tggaagagca tgggccgttg
    11701 cagtgttgca tgcgaccacg agcattttga tgtcgctctg ccccagaaag gctgcaattt
    11761 ctagagaata gcgtaggacc gtgcgaggcg atctggatcc gtaaggaaca cgcgccgtgt
    11821 ctccgaggta aatgaatcgt tcgttcggaa gccggcgcac caattcggac agaacggtca
    11881 gcccgcccat gcccgaatcg aacacgccaa tggggcgtga atcagccatt ctgatccagg
    11941 ccaaatgcag tgtgtgccgc ctgcagtgct cttttggcct gatctttttg cacaacgact
    12001 gagatcttaa tctcagatgt gctgatcatt tcaatgttaa tattttcttt cgccagtgcc
    12061 tggaacatgg tggcggcaac accagaatga gatttcattc caacaccaac cgcggagacg
    12121 atggcgatgt tctcctctac cgcgattcta cccccaccca gttccgcgag gaacttctct
    12181 agaatgccac gtgcattcgc aagatcgtta gatggaactg tgaacgaaat agtgttcttc
    12241 ccgtcatgtc cactgctttg aacgatcata tcgacgttga tgcttgcagt ggcaagtttc
    12301 tcgaaaagtc cagcagccag tcccggtctg tccggtatat cgagtgctgc cacacgtgcc
    12361 tgatccgatt taaggctcac gccgcgtacg agtacttttt ccatatccat gattttttcc
    12421 tccggcacga ccagtgtgcc cgggttgtga ttaaaacttg atcgcacatg cactacgatg
    12481 ccaaacttgg acgcaagctc cacgcttcga ttgtgaagaa cgcccgctcc catgctcgcg
    12541 agttccagca tttcttcgta gcagatttgt ttcaattttt tagcttttgg aactttgttt
    12601 ggatccgtag tgtagacgcc gtccacgtcc gtgtagattt cgcattcatc cgctttcact
    12661 gcaactgcga gagccactgc agtcgtatcg cttccgccac gacccagggt catgatttcc
    12721 tgattttcat tcacgccctg aaaaccggct acgatacata ctttcttctg cgcgaaggct
    12781 tcatccagtc tggtcgtgtc cagtccgcga atgcgagcgt ttgagtgagt cccatctgtc
    12841 atgatctgca gctggccacc cgtgaatgaa atcgcgggga cttcaattgc ctcaagagcc
    12901 atggccatga gagccataga gatttgttct cctgtggaca gaagcatgtc catttcgcgc
    12961 cggggaggat tcccgttgag cttcatggcc atgtcgatga gttcgtccgt cgtgtggccc
    13021 atggcggaca caaccacaat gagcctgtgg ccctgctcgt agtagccctt gatgcgattg
    13081 gcaacattct tgatgcggtc cgtatcgcct acagaagtgc ctccgaattt ctgaacaatg
    13141 agcataatgc tagatctttc caggcgtccg tgcggacaag atttttagaa acagtagccc
    13201 acccatccgc cccaaaaatg attgcaaaga tcgaccaaaa accggtcctg caacctccat
    13261 ccaaaacaga atgaagcaat cgagcaaatc tcatcgccag aaggccacga aacctgtaga
    13321 cacattgcag aggctcctcg gtcgccgggc caccacggtg cgatatcagt ctggctttct
    13381 tgttcggggt cgccttcatc cgttcctgga tctggcgaaa gaccagggcg acgccggctg
    13441 gtccaacgac atagcgaatg atctggaaga gcattcacag catcacttca tagacgtgta
    13501 caacagacgc ctcgcagtgg aagggttgcg cccggctcta tctataaaag gcgctgcgtt
    13561 cctggatgct ggctgttcat ccggttacct tctggcagac gtccaggcgg ctttcccgga
    13621 tgcctctttg ttcggtgcgg acttctttcc tgccgggctc gcacactgcc atcgagcgca
    13681 cccggaaatt cctttatttc gcatggatct cactgcctgc ccgttcccgg acaactcttt
    13741 cgatgcattg agttgcctga acgtccttga gcacattcag gatgatctgg ccgctgctcg
    13801 aactctgttt cgcatcctaa agccggccgg cctcgcagcc atcactgtcc cggctgctcc
    13861 tcacctgtat gatcttttcg atgagattca ttttcatgaa agacgttatt cgctcttgca
    13921 aacgcaagac ttgctgcgtg ctgctggatt caagattcgc tttgccaact actttggatc
    13981 tctgatctac cctgcgttct atgcagtcaa acgaagaaac caaaacaggt acggagcact
    14041 gagctttgag caaaaacgag acctggcaat gagccagatt cagaaaacgt ctcaatccag
    14101 aatcatgaat gcagtctgtc gcttcgaaga agcaatcgga aagcgcctga cgtatccatt
    14161 cggaatccgc gcctgtgtga tcgcagaaaa accgccaggt tgaacctgag atccttcctg
    14221 cgaagtcaac tgcaggctat gcgtaaacct tcattcacgg aaaaagatgg ctcatagccg
    14281 catgccttca agcgaccaat gtcaagagtg aactcaccgg ccaccttgtc gtacatgcct
    14341 gggcgcagaa ttcccatcac tctcaggggg aaaagtggaa atcgaaaact gaaatcagaa
    14401 ctccgggagg cacgtgcaat gcgatgagcc agatccgcaa gagatacatc ctcgtcgtct
    14461 gttacgttcc agattccgga tcgctcactc cgcaaaaaca tctccgcggc agaaagcagg
    14521 ttatctacaa acacatagct tcgcagactg cgaatgcttc ccacaggcag cggccatcct
    14581 ttctgtactg cgcgaatcaa gaaacggaga ctgcctttgc tgccaacgcc ataaaccgga
    14641 ggcggacgta gaatcacaaa gcgatctgtg gccttttgaa tttcatcttc cgccctcacc
    14701 ttcgcccgag agtatgcatc ctgcggattc aatggcgacg cttccgaaaa atgtccggtc
    14761 tccccataca ctttggaact gctgatgaaa aggaatggaa cagatcgtct tttcgcagca
    14821 ttcgcaagct cgcgcgtcca gcgaacgttg actctctcga gggctccttc atctactccg
    14881 cgcgaatgtg caagaccggc aatgtgaaaa atcccatcca cgtccttgca ggcgccttct
    14941 attgcatcct cggtctgaag atctccgact acaacgtctt gatcaggaat gggtgagcgc
    15001 gaatgtgcgc gcacaagata gcccgcattc cgaaaaaacg agcaagcgcg cgatcctata
    15061 aaaccactgg cccccgtgat caatatggtc ttcaaaaccg agcggcctct tgcctgtaca
    15121 tagcttcgcc ccgggagcca caaaatcgcc tatcgcgcac taagaaccat atcgactgaa
    15181 ttgcgcgcat ttgttcctca ccgggcctgc ccgggtggac gacgaaaata ctggaagagt
    15241 tttcctggaa tcttggatag aacaagaagc cagctggaag ggtaaccgtt ctctttcaag
    15301 gccctcagca cttcttttgt cagattaaaa cgatgacgca gactgcgatt gctgagtccg
    15361 ccggttcgca tgcgaaccag aatttccggc agatagcgaa acttgaccct ggccttcaaa
    15421 agaaaccgca gaatgagttc aaagtctccc gcgtaacgat agtcaggcgt gtacgatccg
    15481 agtttttcaa agagatggcg gcgtgcataa aaaccggcat gcggtggcat gatgccacgc
    15541 gtgagatcct cggggcgaaa agcagatgcg gaatagtagc gaagaacttt cccaaaatcc
    15601 tgatccgtta tgatcacgtc tccgattacc acatcaatat ccggtgcaaa tgcactcgct
    15661 actttttcca cgctctcatt ggattcaaat cgatcatctg cattcaggat ggccaccaca
    15721 tctccgtgag aggcagcgat tccttttcgc atcgcgtcat acatacctct gtcgcgttca
    15781 gagatgatct gatagtttag agtgctctgt gaaagaagct ctcgaacaac atccaccgtt
    15841 ccatcctgcg atgcgccatc cactacgatg tactccacag gatgagtctg attcatcaca
    15901 gatcgcaccg tatctcgtat ggtcgcttcg ctgttaaagc agacagttac aatgctcacc
    15961 gaaggcttat tcatttaagc gcctgataga ttttatgata gccagcgacc gttttctccc
    16021 atgagaattt ctcggcctgg atttgaccca gtgccgcata acgctcgcgc gcacctttct
    16081 gacaaacttg ctcgagcgca cgcacaagtc caggtagatc tccaggcaag aacaacaaag
    16141 ctgcattgcc cgccacttcc ggaatcgaag aagaatcgca tgcaaccaca gggcaccctg
    16201 cagtcattgc ctctacgacc ggcaatccga aaccttcgta gagagaagga tacaccagag
    16261 ctttcgcatg agcgtacaat gcttgcattt cgcctggtgt tgcccccggg atactcttca
    16321 catacgttgc gatttggttg tctgagaaac tcttctgttc tgcctcagag aaaggagagc
    16381 cggtgcaaac aaggtcgtat tggtcaaaca ggcgcgcctc tgtcatggcc tgaaccacga
    16441 gctcgaactg tttgtatccg taacgatgtc ctacaaaaag gaaaaagggc ctggtttcta
    16501 tgtgcgcggt cgcacgcccg ggtgcacctg gttcagaatg tcccgcagcc agatgaacca
    16561 cgctcgcacg attctctgcg tctggataaa atcgtagaag atcctggcgg gtgttttcag
    16621 atatacagac cactgcgtcc gctcttcgaa tggcagcgcg cttctgccag atatgaagat
    16681 gcctgaagcg ggattcaacg tgccctctct catagagaaa atcatacacg gtcacaacgt
    16741 tgcgcacgcc gggccagaaa ttcaaacgat agtagctgga atgaaaaacg tcgcagcgca
    16801 tccacaaagg attgtagcgc aaaagtttat gacccgctat ttcttctgtg tccagatccg
    16861 ggtacgattt catgcgcgag cacaaattct gccagaactg agtgatgccg ccgtgggctt
    16921 gcagttgata gatgatgctg tcgagtataa gatgcattaa attcgactca gaaactcaga
    16981 gtctctcacg gttagactgg ggcctggatg aaacagagac acgaaaggcg tacgcgagaa
    17041 ggcaagagta acggcaaagg atagaggatg aatcttacgg cgcgacagaa aatggtaggc
    17101 accaaacgca gaaaccacgt ggagacccag ctctttcgca atgtgtgcga gagatgcagg
    17161 agtaaagaag ccaatgtgct gcccatgctc caaaccatag taccaccact gtccgggttg
    17221 tggcctgtga ggtggaagta gcctcgtaga gaaaaaaatc tccgacgaaa tctgcgaaat
    17281 cttgcggatc tcttcaagag gatgaacaaa atgttcaaaa ctttcaaagc ttgtcaccag
    17341 agaaaacgat ctggcggcgc ggtgcatttc aaagccgcga gcgaagacat tgggagtata
    17401 cggatcgtgc cagtaacact ctgccccggc atcgcgcaac agccgcgtta gaacgccata
    17461 gcctcccgaa taatccagaa caaagtcccc tttcttaaag taacgacgca atagcaacag
    17521 agtcatgcgc gaaagcatta cgttacgccc gagaagacct gtgtcggtga gattcactgg
    17581 attctgatac gcttcttcca accagtatgg atcttccgtt tgaacatatc cacatacatc
    17641 acattcgaag tagtgtacgc ggtgtttggc gagcaaaagt gcttctccaa acgagtgagt
    17701 tgcttcacca cagacgcggc agaccggatt cacaggtatc tccacagctg acgtgccaga
    17761 tagatgagtc gatccactgg cctgacgcga aagacacgat gctctgtaaa ctcgtctgcc
    17821 gcttcgttac gtgcaacgaa tggtggatga cgaagaggcc agtccagttc tgtggttccg
    17881 agcgcctcca gcggactctt gcgttttgta tgtgtagctt ccgccccgaa cccaatgtta
    17941 cttacaaggt ttttgtctgg aagaatggta agcccctgat tcaagagaca ggcgaaggtc
    18001 cactgaatgt cccacgtatt gaagcctttg caaattagat caaaattcct ggcccagtac
    18061 cttccagcag ggcctttgac aagttcccga aatgaatgat tgcgcgaaag ctcgggccag
    18121 gaagcaagat caacatcgta tttctgccag gctcttctcc aggtggccca accccacaca
    18181 tgagggtagc gcgaaaaata gtaggaatag ccccgagact ctggctggaa gttgtccccg
    18241 gaaattgaaa acactctttc atctgtgcga taacgcgcaa gaagctcaga acaaaatcga
    18301 aagaaatcgg gatgtggcag gcaatcatct tccagaatga tcacttcttc tgaccgctcc
    18361 agggcccagc tgatgccaga ggacaatcgc gcgcgacatc ccatgttctc tgtagcaaag
    18421 ttggtttgca cgtcgcaaga ccagtttaca tttgtaataa tacttctgac ttcttcgcag
    18481 agcttgcttt ctgtggcgtt ccgggctcca tccgcaatga gaaagagacg cgccggtttt
    18541 tgagcagcaa ttctctcaaa cactcgtcgc gtaacgtccg gtcgattgaa tgcaaaaaat
    18601 actacgggag tctctagact catgcgatct ctccttcgcg acgaaacccg aagaactcaa
    18661 ccaggggcaa ggcccacact ttgagaaggc ctgtaggaat cgcccagaaa ggcgcggcca
    18721 gaaatagaaa tgtggtcatg tagatcggat tgtgagacgt tttcctggaa aacaccgtct
    18781 tcacataagg ccagaaacgc atggacttta gcagatgatg gccagtcgat tctgtgttca
    18841 tgcgaagcga aacggaaggg tcagaaaaaa taggatgccc tttccttctt gctcgcagag
    18901 tgtattcgac atcagaaagg tagtgcggaa gaagagttgg ataaaatcca ccaatggata
    18961 gaaactcgga aactctcatg catagaccac gagtagtaca ccagtccggc gtctccccat
    19021 ctcgaatcgg acgaaagcgc aatcgaagcc agtccacttt catcctttgc tcttgcagaa
    19081 tatcccggcc atctcttgca gtggcgacga ggagatcccg cggcctttct ttcaataggc
    19141 gttctgcatt ttcaagaaac gcaggatcga gggccgtatc atcattcacg gtcataatta
    19201 cgtcagaagt aggaagctca gcgctccggg cccagaggta cccctgatgc agactgcctc
    19261 cccaccacca gtttcctgta ccgcgaatca cagtagtgaa aggaaaagat tcgcacacca
    19321 tctctgacgt accgtccgta gatccatcgt ctaccagaac gatgtgaaaa tcttttgaag
    19381 aactttgaac aaggctttcc acaaacgcgc gcgtaagttc gatgcgattg tgaacgggca
    19441 gtattatgtg gaccatgatt ctaaaactgg ctctactttg tgatctctgc gtaagtattc
    19501 aagccgattt gttctactcg cgaatccagt cctttcagcg gttcatgccg cgactcgccg
    19561 tatttgatct caaaatcgac actggaaaaa ccaatgtgac ggcacatgag ttccagttcc
    19621 gctcgcgaag caaagtgttt gtgttcaaat acgtcatagg aatcatacac accctgcccg
    19681 taagaagcca gacgattgtc tgagaagtga cgcatcagta caccctccaa acctggaaaa
    19741 gacatgcgaa ggacgcctcc gggtgcaaga acgcgatatg cttcgacgag aaagagaagc
    19801 tgctgttttt gctccaaatg ctcaaagaaa tcctcggaaa atgcatataa aaaccaacca
    19861 tctgcaaaag gatgttccag agtcaaattc gcggacactc tcaccggatc tgcaataaga
    19921 gattccgagg gatagagatc tacattcacc cagccggaca tgagtcttcc gccggacccg
    19981 tagtggattt tcctgctttc gactttcagg attcgttttt ccgcactcac tttccagcga
    20041 ggcttacgcg agatcgtgta tcccgccgca tcaatggcgc gaagaatcag acttttcagt
    20101 ttcccaatca tacatgtccc tggagtcaac gttccactac cggccgaatc ccgctttccc
    20161 tgagcaagca atcatggcgt ttttccggca taactcccat ttttgttttt gcaaaaccaa
    20221 atagagtgtc cacgggcatg tccgtgacga atggcttctg tctggaagtg agatacaaca
    20281 tcgcataacg tccaaaccag atttcaggcg attcaatccg gaccacatgg caactcttta
    20341 catcctcatc aaatgtgtga aaatcccagt tgtggctggc tttgatctga ggatctgcga
    20401 ccggataagg ataagcaaaa tggatgccgc ttttatgagc tgccgcgggg attcgtcccg
    20461 cgccggccca cacatggagc gtaataaaaa tgacggaagc agcatagaac agagggcgag
    20521 tcttctcaaa tgcagaccat gcaagcagcg acacaaagaa aggagaaaaa tagctcaatg
    20581 cttttccggc cgcccagtag gattgccgca gaaccagaaa cagaactatg caggcagcag
    20641 cagaaataaa aacagaaacg gctactgccg gaattgcagt tgaatccaaa ttcttccgcc
    20701 tgcgctcgta gaagtgcgtc acggagtata cgaaccctgc agagagcacg atgcaaaggg
    20761 cactcaacac caggcgcgct gtaaacttcc agatgcgaac ggcatgctcc gtgggcgtaa
    20821 caaaataaaa accaaacaac ccggaaacaa aatcagaaat cccgaaaaat atagatttcg
    20881 caggcaacaa tgaaaatatc tctcgcgcac tcttctgcag aagaaccgga tcaaagtcga
    20941 tcagatcgcg gcccagaaag aaagcctgat agatcttcca ccagtcaaca gcagaagttt
    21001 tcaaaaggcc atttaagtca ttcggttcaa ttttt
//
LOCUS       DAORFF010000004        20583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1025_length_20583_cov_3.964975, whole genome shotgun sequence.
ACCESSION   DAORFF010000004 DAORFF010000000
VERSION     DAORFF010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 20583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20583
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1025_length_20583_cov_3.964975"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..580)
                     /locus_tag="PKK76_00355"
     CDS             complement(44..580)
                     /locus_tag="PKK76_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNL66939.1"
                     /translation="MKVSNLFRIASIALFTVAIAACSSSQSTSGNNTNSNDRSGSKER
                     GGVDMGGALSSLSYSPNGQGFAYKSTYVNPTEFNTWATQFKPQIETALNALAPGFKLQ
                     ITGHTCALGPRDGDGAHLGNVYYSTERAKAVYQALINAGLPKDKLTYKGIANDEPLPG
                     AAPKDQKNRRVTFKIVQG"
     gene            721..1635
                     /locus_tag="PKK76_00360"
     CDS             721..1635
                     /locus_tag="PKK76_00360"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNL66940.1"
                     /translation="MRPDAEGTRLDQWLAAQMASSEEAPSRMQIQRWIKAGLVEGQGT
                     LNPARRIEAGESFTVKPAISEKATDLEPIDLNLKILYQDDELAVIHKPAGIAVHPGPG
                     DGGITLASGLIHAFSELKQGEWEGRPGIVHRLDKPTEGVLLVAKNVKAHRLLSGQFLR
                     REVKKTYAAWLLACPPAEEGEINAAIKRHPKDRLRMRIDPTGRHAITRYKVTRTRVSR
                     RGRKFALVEVDLLTGRTHQIRVHMAHIGCPIVGDEIYSRSSAEFKKFGLLLFAQTLEF
                     KHPTNGSTMRFSLDLPERFDRFENSLEG"
     gene            complement(1657..2706)
                     /locus_tag="PKK76_00365"
     CDS             complement(1657..2706)
                     /locus_tag="PKK76_00365"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNL66941.1"
                     /translation="MKIVQTLTAIVFTSVVCYCGYKPSEAAKTSMLIVFVTGDAFVVS
                     QNKPEVPATVGMIVRENDVIRTESGSVDLQTVHGNAVRIREMTTLTVAAIHSKGETKL
                     GLKNGAVLAKVQKASKEESFIISTPTAVAGVRGTTFSVEIGPDARSSVRVIDGKVGLA
                     PRVAILESMTPEQIKADPTLQKLSTLQSQETVLEDKTQGTLNANLEKQLIRVSETRKP
                     EEIKAEQSVETKTVEIPIRELAESRTLVSVKPQLMDRAIKGDAQAVQEIQSEREMAKE
                     RALDQIMKDASTIELKSEDAIRTRYSRFELITLNSGEKIRGAVIAQTDSSLVVHTTSG
                     VRKIPRAELNFQEPL"
     gene            2818..3411
                     /locus_tag="PKK76_00370"
     CDS             2818..3411
                     /locus_tag="PKK76_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66942.1"
                     /translation="MQPIRFYLILTGVLLVGELTAQVPVKNQPPAGWCSDIRAQVRKE
                     APAVPALLALKATAPTADLQKALTSSDWIIIGDYSYPEQKVNPYYGVSSEQMDFHRFA
                     ADGGMLSFSCYIFKDGKEKTTHINFTAPDTTWNVKKIGTAYYIEEIAHGEKAYFPIIQ
                     FKDNILVVDISSKHSSFPLVPQCAFLSSHTLPLAVEK"
     gene            complement(3299..8323)
                     /locus_tag="PKK76_00375"
     CDS             complement(3299..8323)
                     /locus_tag="PKK76_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNL66943.1"
                     /translation="MQGPEIPGFATGPVLLAHRGIVWHRASREGRDFLLKTANLSSAE
                     SSTLLQREFNLLRTKSIAGIPKPLEFLRMGHQATIVFDGEAGEVLSRVLAREKAMEIL
                     RLLDVAVAIVEVVQSFHANGITHRSLTEERILLHADGRVVVLDFHNATTFVQERAPAL
                     MAAADMDMHYLAPEQTGRTNLLVDFRADLYALGALFYKMLTGRLPFASADSLELVHAQ
                     LASMPAPPSELVGSIPKVMGDIVMKLLAKSPDDRYQSAEGLLYDLNQCRAALRTEGVI
                     PDFVIGGRDFSSTLHLPDDLFGREDQKQTLQRLVRSAVSGQSSIALVMGEPGIGKSAL
                     VASCLTDVFSARGVVLNGRFDSLGRDRPYHGIRQAIQSVVNQMMGESEELLSETRTRL
                     VADVAEYASVLVSAFQELSEILQSEGSGNMSGAEFDRQLEEGFVRLLTSLAGGGPVVL
                     ALDDVQWADHASRRLISRMLSRRNMGSLLCLLSVREQEIDPLHPVLQIMKEQDNEHTV
                     VIRLGPLSEEDIFEILTRTLRTEGAPVRQLATLLRAKTEGNPFFLRQYLRDLGNDGTL
                     KAIDGKWVWDYSAIQSRSIADNVIDLVVTKILYEKGEAQSLLKLISCLGSRAPVDLLA
                     ISTGRTSADLEAILEPLVRNGFLVLEGRNVSFVHDRVREAAHTLMTDDERSSNHLVLG
                     RALLRDHTIIGDAEYTIPAVLNPVMHLLNPAERLELARLNLAAGSRARSGGAFSEAGR
                     YFSQGYSLIEDANWDTDYELSFALALGLAESSYLNEEHAKAVDLFQVCSGRARDPLDS
                     ARVQELRALLLLSEGKLGEAIDAGLTGLDTLGIRLPRHPGQMGPLPDLIACRLWFVRR
                     KIEDLSYLKDASDSRIRAAMHLMMIIGNAAFIGRPALVPVVIARMVRISLRYGFFEET
                     TFAIVMFGLIIYALGDPSSAIRFGATGAAMLSRVKSKEITGKVLYIHACFIRHLSEHG
                     GKSIEAFLEGYSYSVTAGDTQFQSYNANHYCIQHLLYGGHFGEALEQFSRFTTLLEQA
                     GNFDPIQYHKAFKQFAKYLTTGGQNVSTSLEGADFDVATAERKLKESGSFTVLFPIYM
                     LQGVLELLSGNYAGAIERFTEMRKFESGALGMPFVPHSCMFESLAALDYASLHSADRD
                     ALIRRARKLLKRLKTWSKLGPENYLHKALLVEGAIAEARDEIHRAFELYDHAIERSLE
                     GGFVFEAALASERAGKMYRRIGRERLSRIYASSAHDLFQRCGATYCADRLRGEWPGVD
                     DVLVGARSADTSLDFASVMKAARAISEEIRFDTLLEKLLSIMMENAGASRGAIVIEDR
                     GHLTVEVVMDAGQTTNQVTQSTALASAKALPLRVVYFVQRTRRSVILENAYRNSEYGS
                     EPYIASHHVRSLACLPIVNRGKLQGIIYLENRHTAGTFTSERLEILGFLTGQAAISIE
                     NAIVYRDLEAKVSERTAMLNEKKSELEKSLSVVEKLKSEQDCDYYLTSLLLEPLAASR
                     VDTGEIRIDFYLEQKKKFRYRRWESSLGGDICSAHTIELRGARYSVFVNADAMGKSIQ
                     GAGGALVLGAVFASLIQRTKLTRQIQEQYPEQWLKFSFVELQKVFESFEGYMMISMVL
                     GIVNNRTGAAYFINAEHPDLVLLRHGQATFLQPVATYAKIGRRIGGRGENWSVLTRYP
                     PRECCP"
     gene            complement(8400..11609)
                     /locus_tag="PKK76_00380"
     CDS             complement(8400..11609)
                     /locus_tag="PKK76_00380"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL66944.1"
                     /translation="MTEPYSGERGCLSIVMLEFLGRIYWNYFAIGYIVVGAFTLLAGL
                     FAFTIKDRSRATFHFGMFFLAATALSFAFTYGHFFYDTAAVYHRYFTIIGAMSASVHI
                     LQFLIHFPEPSKRKVAWFAWVQWIVIALTVGYFAKVTWSIERTFHFSAQLWDYDAEVL
                     SRNVGFVIIVYALIYVVYGVFKVIFKLKGQPRWVVLAMLIAFISVTLVPGILNVLSRE
                     GAIDRNLFVNIFTLLNIVGCFTVVILFINNTSDRTTFMVKITGITLVTVLFIFQILTY
                     FSLKGMDENFDEIHFREGELFLKGQRSDSVQYAYTHRLSASMSQEPIQANEKEKDKNK
                     EKDAVPVSGGVATVDYREHEHEYENTYILYTISGLGRDRFNQELETILSRSPVHFNGY
                     SNLIREYLRNAREASAADVLERIASVQRLVGYRAFKIRQLPDKTFRKDLTEFLKGSGA
                     PFAPFRAAIEEHLALSKAEGAELKAEVLHFVTPAHMPGFRWYRDSADGRTQFVSYIYV
                     AAPGSVTEVGFSYRGYREFMHHNASRAVILLIAALLFVLIGFRVFFSGALLGPLEALL
                     RGVRKVNAGNLDVAVTARVEDEIGFLTHSFNGMVSSIRDARLKLQDYANSLEEKVKAR
                     TAELQETLTQVQSLKSQQDGDYFLTALLLRPLRANRAKSDAVSVEFFQRQKKHFTFRH
                     WQEEIGGDLCMSNNLRLNGRPFTVVMNADAMGKSMQGAGGALVLGSVFEAILERTQLS
                     AAMQEQFPERWIKNAFIELHKVFEAFECSMMVSLVIGLIDEESGLLYYINAEHPFSIL
                     YRRGKAAFMDVETLFRKLGSPATSGNISIRTFQLQPGDVMIIGSDGRDDILLSEEGRE
                     PFMNEDESKILGHVEASDGNLQGIFDRLTSWGTIIDDLTFIRIGYRESGGVPEALIES
                     PVNKEIRDLLVKSGELTRAQNFAGAVPLLEQAFKLDDRHPEVIKRLALAYVNLKMFDK
                     AAPLAEDYTYLRPGDSEFVYVASYCLKKVKEYSRAADYGERLKLRNPRHVKNLINLAE
                     VYAKMRNLARAELLLDSALLVDPDNTTARSRLEKVRRAA"
     gene            11708..12055
                     /locus_tag="PKK76_00385"
     CDS             11708..12055
                     /locus_tag="PKK76_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66945.1"
                     /translation="MDLAGRILHWLSRILFPIAFIPLLFPYTTVLFIVLVFAAVIVYL
                     GERRSGGLPLTQSRSFHVMRLSSIAVLLNAALFFGLFLYSQTCILRPLSQQLDPLREY
                     YRQQGQTQSETKL"
     gene            12052..13320
                     /locus_tag="PKK76_00390"
     CDS             12052..13320
                     /locus_tag="PKK76_00390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNL66946.1"
                     /translation="MNVVIFTDTYLPKVDGVGISVDHFTRILGGRGHNFVICAPRYSD
                     HDPETISDHIHVIRFKNAPLPSYPDIKVVIPSHKKLTKAMTMFPPDLVHIQTPGLLGQ
                     YGVLAAKMYGVPMIGTYHTLVSEQDTYISLYRLLKVDTLLNYFKSHKKIEKRLDKIER
                     KKKEKTLKSQLIYKLTLGLYQAGELIISPSHLIKKDLVEGGASIPIEVVSNGMDLDRF
                     RGEIKEPRKPPKFLHVGRISFEKNCEVVLKAFALILEKIPDATLDIIGDGPALTSIKI
                     EAKQIGVFDRCNFPGFVAHGSLHEIYPKYDLFLTASTMETQGLVVLEAMACGLPCVGV
                     NAYALPELIQHERNGFVVEPFDHIHMAERALQLLGDPELYKKFSAQSLAIAGEHELNS
                     CADRLESVYHSVVEKHRKKPAGLFGGLLPG"
     gene            complement(13328..14017)
                     /locus_tag="PKK76_00395"
     CDS             complement(13328..14017)
                     /locus_tag="PKK76_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66947.1"
                     /translation="MNYFEIEKNLQVALDDGQKIEIISFRVDEAVEKQLLLTVEKILE
                     RYSRAHLKDALFSCALELATNATKANMKHVYFTERGLDIDKPEDYEKGMAGFKDVVLT
                     RPWLEAYAEKARSLGLHVQMIFTHTDDGLRIEIINNRPILAQDEERIREKFQKAMTYD
                     SLHEYFLDHADQTEGEGLGFALNILLLKGENLDATLFRIGREERGTIARIEVPFTDKF
                     VSVRPKEKPDA"
     gene            complement(14088..14825)
                     /locus_tag="PKK76_00400"
     CDS             complement(14088..14825)
                     /locus_tag="PKK76_00400"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNL66948.1"
                     /translation="MIRAFVLFFALITSAYAEGTLYVLCYHSFQGRKTDYDVAPEAFE
                     KQMQQVHDLGFRFVRTEDVLADRVNGAKNVLVSIDDGNKSVEAIYDRVLKKHDIKPLL
                     FIYPNIINRSNVPYAMTNDQLNRYLREGATLGAHGYYHLFLNEKQYNKDKKGFLKEIY
                     DSRDTLESRFGHRPEVFAYPFGVFSDLARDEIKKAGYKYSFTIVQGPVLLPLARNADP
                     FKLPRFLVTKTSWKNVLAVLERAVRNR"
     gene            complement(14828..15460)
                     /locus_tag="PKK76_00405"
     CDS             complement(14828..15460)
                     /locus_tag="PKK76_00405"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HNL66949.1"
                     /translation="MPVEICAMRVFHEVAHPWHGIHPGEQAPELLTAYIEIVPLDTVK
                     FELDKTTGHLIIDRPQQYSSLCPTPYGFIPRTYCGDGIAALARAKNKKIEKGDGDPLD
                     ICVLAEATITRSDILLRARPIGGLRLIDRGEADDKIIAVMRGDALFDACTDIKHIPET
                     LLDRLRHYFLTYKQIPGAGPRAVEIAEIYGVETAHEVIRVAMNDYRVSYT"
     gene            15528..15950
                     /locus_tag="PKK76_00410"
     CDS             15528..15950
                     /locus_tag="PKK76_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004770159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNL66950.1"
                     /translation="MYQKGKTFDQIAVGEKASFTKTITETDIYLYAGISGDFNPLHVD
                     EEYAKTTPFKGRIAHGGLAGSLLAPVLGMKLPGLGTMALEVTQKYRSPVFPGDTITCT
                     VEVTEKIARMKAVDMKILWTNQKGETIAKGNCRVLPPA"
     gene            16007..16792
                     /locus_tag="PKK76_00415"
     CDS             16007..16792
                     /locus_tag="PKK76_00415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNL66951.1"
                     /translation="MTERKEVALITGASSGIGQDIARILAEKKIDLIITARRRDRLDA
                     LAKDLRTIHNIQVDVIESDLAKPDGAVQLYNQARAISKDITIVVNNAGFGKQNPVLDQ
                     SIEEIRSMIQVNMTSLTELTRLFAADMKAQGYGRILQLSSIGAFQPSPIYAVYSAAKS
                     YVLSFSYAMNHELKGSGVSITTMCPGITETEFHDVAGHEKTGFMKSFSMTSRKVAEIG
                     VRAMMKRKANVIPGFSNAINSWLMESMPRAVSTSIAASLMKKS"
     gene            17083..18996
                     /locus_tag="PKK76_00420"
     CDS             17083..18996
                     /locus_tag="PKK76_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNL66952.1"
                     /translation="MSELLITKHIPDMYARAARLFAQRPAFATRVKGTTDWQSITFQE
                     LYETGLDLATALIDLGVKARDHVALLADNRLEWIIADYGIQLCGAADVPRGTDVTDMD
                     IQYILPHSEAKVVFVEHLEMLKKLDKNKRKLSKIKHIILMEKSAKAPKNVLSMYDLID
                     KGRAMRARGNKKAEERIAGIKPDDLFTLIYTSGTTGAPKGVMLTHANICSQFKHEIVP
                     LNDHDRILSILPVWHIFERGFEMLAISRGCCTYYTNVRSLGEDIKLVKPTFMGSAPRL
                     WESIYLKIMDGVKSASPVKRGMFRAAYFCSRMFQGSLFFITGKKLDMEGRNPAVSAIT
                     GIGHALRAVVFAVPYFLLDMIVLKKMRTATGGHIKGTVSGGGALPLHVDEFFNYIGIP
                     VLEGYGMTETAPVLAVRNFEKRVIGTVGPVFPQTEIRIIDLNSGDVLFPNPKNRADGR
                     GLKGEIHAKGPQVMAGYFKNPEATKRVLRDGWMNTGDIGMITFNDCVKIMGRSKDTVV
                     LIGGENVEPVPIENKLVESQYIDQCMVVGQDQKFLAVLVVPSLDGFARGGLKAKTLSE
                     LASNEEAGKVITAEIKRIVSADQGFKSFERVQDFRFITKPFEVGDEMTNLFKLKRHVI
                     TEKYKKEINSMYK"
     gene            19057..19533
                     /locus_tag="PKK76_00425"
     CDS             19057..19533
                     /locus_tag="PKK76_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66953.1"
                     /translation="MFGLTQLGIVHTLIALIAVGTGAFLLIREGRISPAGLAGKIYVF
                     ATIVACITALAIYQHGGFGKGHVLAILTLVVLGVATAAGRGMFGGVSKAVETVSYSAT
                     FLFHWIPGVSEATTRLPLGSPLMNGPEDPALKTILGVLFVLFVVGSVLQVRSLRKS"
     gene            19598..>20583
                     /locus_tag="PKK76_00430"
     CDS             19598..>20583
                     /locus_tag="PKK76_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66954.1"
                     /translation="MSGNLFLNFFSIGALIPLLFVMFIALLLIRIRDKGESTWWLASF
                     AIVYCLFMLPYFPASVLFHPAGAYHRWFTVPFVLLTFTFLAQFFFHMPSNTHPRFAKV
                     SFFVQCIVALGVTVLFVARTFHAGKVYNFDGHYFDFDAEEISRLVGVFIILYVVYYII
                     IAVFRAVIAKDQERKIILYFLAFFIIATIPPSVTNILSRDGALDRGTHQTVIVACNVL
                     GFFGVMTVFLNTTRDRTTFMAKIISISIITFLTVFQGLNYFVLSDKEETYDELHRKDA
                     ALMLATPYRAADFRYAMVWSMKDAKEKPAFGSVPAVDLDFYKIEIANASILE"
BASE COUNT         5403 a         5285 c         4899 g         4996 t
ORIGIN      
        1 cggccctttg agccgctttt cctgagtcgt cagtagacta gaattagcct tgaacgattt
       61 tgaacgttac acgacgattc ttctgatctt ttggagcagc gcctggaaga ggctcgtcgt
      121 ttgcaatgcc cttgtatgtg agcttgtctt ttggcagacc tgcgttgatc agtgcctggt
      181 aaactgcttt cgcacgctca gtagagtagt atacgttacc caggtgtgcc ccgtctccat
      241 cacgaggacc gagcgcgcaa gtgtggccag tgatctggag tttgaatcca ggggccagtg
      301 cgttgagtgc agtttcgatt tgtggtttga actgtgttgc ccaggtgttg aattcagttg
      361 ggtttacgta ggttgatttg taagcgaagc cctgtccgtt tggagaatac gacagactgc
      421 taagcgcccc gcccatatca acaccgccgc gttctttgga tccgctacgg tcatttgaat
      481 ttgtgttatt gccgctcgtg ctctgggaac tgctgcaagc agcaattgct acagtgaaaa
      541 gagcaatgga agcgatgcga aataaattgg aaactttcat ggttttgcac ctcatggtat
      601 atgagtcatt ttaaaatccc gaatcaggca tggaaatctt tttttaagcg tcaaacaggg
      661 gcccggtgaa ggagttggaa cagcgtaaat ccagtggaaa aaaatgaaca aattttcatc
      721 gtgcgccccg atgcagaggg aaccaggctc gaccagtggc tggccgctca aatggcttcg
      781 agcgaagagg cgccctcaag aatgcagatc cagcgatgga tcaaagcagg tcttgtcgaa
      841 ggtcaaggaa ctctaaaccc agcgcgtagg atagaagccg gcgaaagctt cactgtaaag
      901 cctgctatat cggaaaaagc gaccgatctg gagccaatcg atctaaacct gaagattctc
      961 taccaggacg acgaactcgc agtcattcac aagcccgccg gaatcgcggt gcatcctgga
     1021 ccgggagacg gcggaatcac tctggcaagc ggcctcatcc atgctttcag cgaactgaag
     1081 caaggcgaat gggaaggtag acccggaatt gttcatcgcc tcgacaaacc aacagaaggt
     1141 gttcttcttg tggcgaagaa tgtgaaagca cacagacttc tctctggcca attcctcaga
     1201 cgcgaagtaa aaaagactta tgctgcctgg ctccttgcat gtccaccggc agaagagggg
     1261 gaaatcaacg cagccatcaa gagacatccg aaagatcgtc ttcgcatgcg aattgatcca
     1321 acaggacgtc atgcaatcac gcgatacaaa gtaactcgca caagagtttc gcgtcgtggt
     1381 agaaagttcg cactcgtaga agtggatcta ctaacaggac gcactcatca aatcagagtg
     1441 cacatggccc acattgggtg tcctatcgtt ggagatgaaa tctattctcg cagcagcgca
     1501 gagtttaaga aatttggact tctcttattt gcgcaaacgc tggagttcaa acacccgacc
     1561 aacggcagta ctatgcggtt tagcctggat ctcccggagc gatttgatcg tttcgagaat
     1621 tcgctggaag gttgatcctt ccagcgccgt gacctcttac agaggttcct ggaaattcaa
     1681 ctcagcacgt ggaatcttgc gaacaccgga cgtagtgtgg actaccaggg aactgtctgt
     1741 ctgcgcaatc accgctccgc gaatcttttc cccgctgttt agagtaatca attcaaagcg
     1801 cgaataacgt gtgcgaatcg catcttcgct tttcagttcg atggtggatg catccttcat
     1861 gatttgatcg agagcacgct cttttgccat ttcgcgttcg ctctgaattt cctgaacagc
     1921 ctgagcatct cctttgatgg cacgatccat caactgtggc ttcacagata caagagtgcg
     1981 cgattctgcc agttcacgga ttggaatttc aacagtcttt gtctccacac tctgttctgc
     2041 tttgatttct tccggtttgc gagtttcaga tacacggatc agttgctttt ccagattcgc
     2101 attgagagtg ccctgtgtct tgtcttcgag gacggtttcc tgagattgca gagtagaaag
     2161 cttttgaagt gttggatccg ctttgatttg ctctggagtc atggattcaa gaatagcaac
     2221 tcgcggagca agcccgacct ttccatcgat cactcgaacg gaagaccttg catccggccc
     2281 gatttcaacg gagaaagtag taccgcgaac accggccact gcagttggag tggaaatgat
     2341 gaaggattct tccttgcttg ccttttgcac tttcgcaagc acagcgccat tttttaaacc
     2401 gagctttgtt tctcccttag aatggatcgc agcgacagtc aatgttgtca tttctctgat
     2461 gcgaacagca ttgccatgca cggtctgaag gtctacggat ccgctttctg tgcgaataac
     2521 gtcattttct ctcacaatca ttcccacagt tgcgggaacc tccggcttat tctgcgagac
     2581 tacaaaggca tctccagtca cgaatacaat caacatggat gttttcgctg cctccgatgg
     2641 cttatatcca caatagcaga cgacagaggt aaaaacgatc gcggtcaggg tctggacgat
     2701 tttcatatga ttgctccatc tacacaacaa acagtgggcc ggttttttgc cagattttct
     2761 ccagaattag tccggaaaaa agggatgcga accccgaaga atgagtctcc atgacgcatg
     2821 cagccgattc gattctactt gattctcaca ggagtgcttc tcgttggaga actcacagct
     2881 caggtgcctg ttaagaacca gccgcccgct ggctggtgct ctgacatccg cgctcaagtt
     2941 cgaaaggaag ctccggcggt tccagctctt cttgcgctga aggcaaccgc acccactgca
     3001 gatttgcaaa aagcactcac atcatctgac tggattatca tcggggacta ctcttatcct
     3061 gaacaaaaag tcaaccctta ctacggagta agctcggaac aaatggactt tcatcgcttc
     3121 gcggcagacg gaggcatgct ttcattttct tgctacatct tcaaagatgg aaaagaaaaa
     3181 acaacacaca tcaacttcac agctccggac accacatgga acgtgaagaa aatcggaacg
     3241 gcctactaca tagaagaaat cgcacacgga gaaaaagcat attttcctat cattcaattc
     3301 aaggacaaca ttctcgtggt ggatatctcg tcaaaacact ccagttttcc cctcgtcccc
     3361 caatgcgcct tcctatcttc gcatacgttg ccactggctg tagaaaagta gcctgaccat
     3421 gacgcaagag aacaagatcc ggatgttctg cgttgatgaa gtaagccgcg cctgttcggt
     3481 tattgacgat tccaagaacc atggaaatca tcatatatcc ttcaaaactt tcgaaaactt
     3541 tttgaagctc tacgaacgaa aatttgagcc attgttctgg atactgttcc tggatttgtc
     3601 gagtcagctt cgttctttga atcagagatg cgaaaacagc cccgagaacc agagcgcctc
     3661 ctgcaccttg aatcgatttg cccattgcgt ctgcgttgac gaataccgaa taacgggcgc
     3721 ctcgtaactc tatggtgtga gcactgcaaa tatctccgcc aagagaactc tcccatctgc
     3781 gatagcgaaa cttcttcttc tgctccagat aaaagtcgat tctaatttct ccagtatcga
     3841 cgcgactggc ggcgagaggt tcaagtagca aagaagtaag atagtagtca caatcttgct
     3901 cagacttcag tttttctact acggaaagcg atttctccag ttcgcttttc ttttcattga
     3961 gcattgccgt acgctcggac accttcgctt ctagatcgcg gtaaacaatg gcgttttcaa
     4021 tcgagatggc ggcctggcca gtgagaaagc ccaggatttc gagtcgttcg gaagtgaagg
     4081 tgccggccgt atgccgattc tccagataga tgatgccttg aagttttcct ctgttcacga
     4141 ttggcaaaca ggcaagagat cgcacatgat gcgacgcaat atatggctct gaaccatatt
     4201 cagagtttct atacgcattc tctagaatga cactacgacg agtgcgctga acaaaataaa
     4261 cgacgcgaag aggcagggct ttcgcactgg caagtgccgt ggattgtgtc acctgattgg
     4321 ttgtttgacc ggcatccatc acgacttcta cagtcaggtg tcctctgtct tcgattacga
     4381 tggctccgcg agatgcacct gcattctcca tcatgatgct gagcaatttc tcaagcaatg
     4441 tatcaaatct gatttcctcg gagatcgctc ttgcagcttt cattacagat gcgaaatcca
     4501 gagacgtgtc tgcagaacgc gcgccgacca gaacatcgtc gacaccgggc cattcgccac
     4561 gaagacgatc cgcacagtag gttgcaccac atctctgaaa cagatcatga gcggagcttg
     4621 cgtaaatccg cgagaggcgc tctcgcccaa ttcgtctgta catcttgccg gcacgttcag
     4681 atgccagggc cgcctcgaac acaaacccgc cctccaagct gcgttcaatc gcatgatcgt
     4741 acaactcgaa ggctcggtga atctcatcgc gagcttccgc gatcgcacct tctacaagga
     4801 gagctttgtg cagatagttt tccggcccca gctttgacca tgtcttcagc ctcttgagca
     4861 actttcgggc tcttcttatc agcgcatcac gatccgcgct atggagcgat gcgtaatcca
     4921 gtgctgccag agactcgaac atacaggagt gaggaacaaa tggcatcccc agagcaccag
     4981 actcaaactt gcgcatctca gtgaagcgct cgatcgcgcc cgcataattg cccgacaaaa
     5041 gttccagaac gccctgcaac atgtagatcg ggaacaacac agtgaagcta ccggactctt
     5101 tcagttttcg ctctgctgtc gcgacgtcga aatccgcgcc ttcgagagac gtggacacat
     5161 tctgtccgcc ggtcgtcaga tacttcgcaa attgtttgaa tgccttgtga tattgaatcg
     5221 ggtcaaaatt cccggcttgc tccagcaatg tagtgaagcg actgaactgt tccagtgcct
     5281 ctccaaaatg accaccatag agaaggtgtt gtatgcaata atgatttgca ttgtaagatt
     5341 gaaactgcgt atcgcctgcg gtcactgagt acgaatagcc ttctagaaac gcttcaatgc
     5401 tcttgccgcc atgctcagag agatgccgaa taaaacacgc gtgtatatag agaaccttcc
     5461 cggttatttc cttactcttg actcgagaga gcatggcagc ccccgttgct ccgaatcgaa
     5521 tcgccgaaga tggatccccc agggcataaa tgatcagacc gaacattacg attgcaaaag
     5581 tagtttcttc aaagaaacca taacgaagag agattcgcac catgcgcgca atcactacag
     5641 gcacaagcgc aggtctgcca atgaatgccg catttccgat gatcatcatg agatgcatcg
     5701 cggctcgaat ccgcgaatcg ctagcgtcct tcagataaga cagatcctcg atttttctgc
     5761 gcacaaacca caagcgacag gcaatcaaat caggaagagg acccatctgt cccgggtgcc
     5821 gcggcaggcg aatcccgaga gtatcgagtc cagtgaggcc agcgtcgatc gcttcgccga
     5881 gttttccctc actcaatagg agcagagcgc gcaactcctg aacgcgagca gagtcaagtg
     5941 gatcacgcgc tcgccctgaa caaacctgga ataaatccac agctttcgcg tgctcttcat
     6001 tcaagtaaga gctttctgca agtccgagag caagagcaaa cgaaagctcg tagtcagtat
     6061 cccagttcgc atcctcaatc aaagaatatc cctgcgaaaa gtacctacct gcctcagaaa
     6121 aagccccacc acttcttgcg cgagaacctg ccgcaaggtt gagccttgca agttccagtc
     6181 gttccgcagg attcaacaaa tgcatcactg gattcaggac tgccgggatt gtgtactcag
     6241 catcaccaat gatggtgtgg tctcgtagca gcgcgcgccc caggacaaga tgattggaag
     6301 acctctcatc atccgtcatg agagtatgag ctgcctctct cacgcgatcg tgtacgaacg
     6361 aaacgttgcg accttccaga accagaaacc cgtttcgaac gagcggttcc agaatagcct
     6421 caagatccgc agaagttcgt cctgtagaaa tcgcaagtag atcaacagga gctctagatc
     6481 caaggcagga aatgagtttg agcaaagatt gggcttcgcc tttctcatac aggatttttg
     6541 taacgaccag atcaataacg ttatccgcta tactgcgcga ttgaatcgcc gagtagtccc
     6601 agacccattt tccatctatt gccttcagtg tgccgtcatt gcccagatca cgcagatact
     6661 gacgtaagaa aaacgggttt ccttctgttt ttgcgcgtaa gagcgtagcc agctgtctaa
     6721 ccggcgcacc ctccgttctc aaagtccttg ttaaaatttc gaaaatatct tcttccgaaa
     6781 gaggccccag gcgaatgaca actgtatgct cgttgtcttg ttctttcatg atttgaagca
     6841 caggatgcag aggatcaatc tcctgctctc gcacagacaa caaacaaagg agcgatccca
     6901 tgttgcgacg cgaaagcatt ctagaaatca atcgacgact ggcgtgatcc gcccactgaa
     6961 catcatcgag cgcaagaaca actggcccgc ctccggccag actcgtcaga agacgaacaa
     7021 aaccctcttc gagttgccga tcgaattcgg ctccggacat gttcccggat ccttcagatt
     7081 gcagtatttc agaaagttcc tggaaagctg aaaccaggac actggcgtat tccgcgacat
     7141 ctgccaccag tcgcgttcgc gtttcagaca gaagctcttc gctttccccc atcatctgat
     7201 ttacgactga ttgtatagcc tgtcggatgc cgtggtaggg tcgatctcga cccaggctgt
     7261 caaatcggcc gttcaagaca actccgcggg cggagaatac atccgtcagg caagaggcta
     7321 cgagcgcgga cttcccaatt ccaggctctc ccatcaccag agcaatcgag ctttgaccac
     7381 tcaccgcaga acgcacgagg cgttgcaagg tttgcttttg atcctcgcga ccgaagagat
     7441 catcgggcaa atgaagagtg gagctgaaat ctctgccgcc tattacaaag tccgggatga
     7501 ccccttctgt gcgcaaagcc gcgcggcatt gattcaaatc gtagagcagt ccttccgcac
     7561 tctggtagcg gtcgtccggg gacttggcaa ggagcttcat cacaatatcg cccatcactt
     7621 ttggaatcga tccaacgagt tccgagggag gagcaggcat ggaagcgagc tgcgcatgaa
     7681 cgagttccaa agaatctgcg cttgcgaacg gaagtctacc ggtgagcatc ttgtagaaga
     7741 gcgcgccgag agcgtaaaga tccgctctga aatcaacgag aagattcgtg cgtccagtct
     7801 gttctggagc aagatagtgc atgtccatat ctgccgccgc catcagcgcc ggggctctct
     7861 cctggacaaa ggtggtggcg ttatgaaaat caaggacaac aacgcgacca tctgcatgca
     7921 aaaggatccg ctcttccgtc aggctccggt gcgtgatccc attcgcatga aatgattgga
     7981 caacttctac aatcgccaca gccacatcga gaagtcgcaa aatctccatt gctttttcgc
     8041 gcgcaagaac cctggacaaa acctcaccag cctctccatc gaaaacgatc gtcgcctggt
     8101 gacccatacg caaaaactca agaggcttcg gaatgcctgc aattgatttc gtacggagaa
     8161 gattgaattc acgctgcaga agagtggaac tttccgctga actgaggttc gcggttttta
     8221 gaaggaaatc acgtccttct ctacttgcgc gatgccagac aattccacga tgagccagca
     8281 agactggacc agtggcgaag ccaggaattt ctggaccttg catatccgaa taactcgtgc
     8341 gcaggtggct gaaaatggca aactaaatcc gagaatccgg aaaagttcgg atcgctattt
     8401 caagcagccc ttctgacttt ttctagcctg ctacgagcag tagtattgtc aggatccaca
     8461 aggagcgcag aatccagaag cagctccgcg cgagcgaggt ttctcatctt cgcgtacact
     8521 tctgccagat tgatcagatt cttcacatgt cttggattgc gaagcttcag gcgttcgccg
     8581 tagtctgcag cgcgcgaata ttccttcact ttcttcaagc aatacgacgc gacgtataca
     8641 aattccgaat cgcctggccg taagtatgta tagtcttctg caagcggtgc cgccttatcg
     8701 aacattttca aattcacgta cgcaagcgcg agccgtttga tgacttcggg atgtctgtcg
     8761 tcaagtttaa atgcttgttc aaggagagga accgcgcccg cgaaattttg agctcgagtg
     8821 agctcgccag acttaaccag taagtcgcga atctccttgt tgacggggga ttcaatcaac
     8881 gcctcaggca ctccgccact ttccctgtat ccaattcgga taaaagtcaa atcgtctatg
     8941 atagtgcccc atgacgtgag acgatcaaag ataccctgca gatttccgtc tgacgcttcc
     9001 acatgcccga ggatcttgct ttcatcttca ttcatgaacg gttcgcggcc ctcttccgaa
     9061 agaagaatgt catcgcgacc gtcagaccct atgatcatca catctccagg ctgcaactga
     9121 aacgtgcgaa tggaaatgtt gcccgaagta gcaggagaac caagtttgcg aaacaaagtc
     9181 tccacatcca tgaatgcagc cttacctctt ctatatagta tagaaaatgg atgttctgca
     9241 tttatatagt aaagaagacc agattcttca tctatcaaac caatcacaag cgaaaccatc
     9301 atagagcatt cgaatgcttc gaatactttg tgaagttcta taaatgcgtt cttgatccat
     9361 ctttccggga attgttcctg catggccgca cttagctgcg ttctctccag gatcgcttca
     9421 aagactgagc caagaaccag agcgccgccc gctccctgca tggatttacc cattgcatct
     9481 gcattcatca caaccgtaaa gggccttcca ttcagacgaa gattattgga catgcaaagg
     9541 tctccgccta tctcttcctg ccagtgacgg aatgtgaaat gcttcttctg cctctgaaaa
     9601 aactcaacgg atacagcatc gctcttcgct cgattcgcac gcagaggacg aaggagcaag
     9661 gccgttaaaa agtaatctcc atcctgttgc gacttcaggc tttgcacctg ggtcaatgtt
     9721 tcctgcaatt ctgctgttcg ggccttcacc ttctcttcca gggaattcgc ataatcctgc
     9781 aacttgagac gagcgtcgcg aatcgaagaa accattccat taaagctgtg cgtcaaaaag
     9841 ccaatttcat cctctactcg cgccgtgacc gcgacgtcca gattgccagc gtttactttt
     9901 ctaacgcctc gaagaagtgc ttcgagtgga ccaagaagtg cgccggaaaa gaacactcga
     9961 aatccaatga gaacgaaaag gagcgctgca atcaacaaaa tcacggcgcg cgaagcgttg
    10021 tgatgcatga actctctgta tccgcgatac gaaaacccta cttccgttac agaaccaggt
    10081 gctgcgacat atatatagga tacaaactgt gtgcggccgt ctgccgagtc tctgtaccac
    10141 ctgaaaccgg gcatatgcgc gggagtgaca aaatgcagga cctccgcctt gagctcggcg
    10201 ccttccgctt tgctcaaagc aagatgctct tcgatggctg cgcgaaatgg ggcgaacggt
    10261 gcaccggaac ccttcagaaa ctctgtgaga tccttgcgaa acgttttgtc aggcagttgt
    10321 ctgatcttaa aagcgcgata cccgacgaga cgttgcacag acgcgatacg ttccagtaca
    10381 tccgccgcag aggcctctct tgcatttctc aaatactcac gaatcaggtt tgaatatcca
    10441 ttgaagtgga cggggctgcg agaaagaatc gtttccagct cttgattgaa tcgatcgcgc
    10501 cccagaccgg aaatcgtata taaaatataa gtattctcgt actcgtgttc gtgctcccgg
    10561 taatctacag tggccactcc tccggaaact gggactgcgt ctttttcctt gttcttgtct
    10621 ttttcttttt cgtttgcctg gatgggttcc tgagacatgg aagcgctcaa tcggtgagtg
    10681 tatgcatatt gaaccgaatc tgagcgctgc cctttcagaa acagctcccc ttctctgaaa
    10741 tggatctcat cgaaattctc atccattcct ttgagactga agtaggttag aatctggaaa
    10801 atgaatagca ccgttaccag tgtaatgcca gtgatcttca ccatgaaagt ggtgcgatca
    10861 gaagtgttat tgatgaataa gatgacgacc gtgaagcaac ctacaatgtt gagaagagta
    10921 aagatattca cgaacaggtt gcgatcgatc gcgccttcgc gagatagaac attcagtatg
    10981 cccggaacaa gagtgactga aataaaagca atgagcattg ccagaacaac ccagcggggc
    11041 tgccccttta atttgaaaat aactttgaat acaccgtaca ccacatagat caatgcatag
    11101 acaatgatca caaaaccaac gttgcggctc agaacctccg catcataatc ccagagctgc
    11161 gcagaaaaat gaaacgttct ctcgatcgac caggtgacct ttgcaaaata gccgacagtg
    11221 agcgcgatta cgatccactg aacccacgca aaccaggcaa ctttgcgttt tgaaggttct
    11281 ggaaaatgaa tcaagaattg caggatgtgc acactggcag acatggcccc gatgattgtg
    11341 aaatagcgat gatagactgc cgcagtatca tagaagaaat gcccataagt gaaggcaaaa
    11401 ctcaatgcgg tggccgccag aaagaacatg ccaaaatgaa aggtggccct ggatcgatct
    11461 ttgatggtaa aagcaaagag ccctgcaagc agagtgaaag ccccgactac gatgtagcca
    11521 atcgcaaagt agttccagta gattcggccc agaaactcaa gcataacgat agatagacag
    11581 cccctctccc cagaatacgg ctcagtcatg ccccaggtca agcgatcctg aaatttttct
    11641 tgccatccgt gggatttcgc ggccaaaaaa ggatagctct tcttcgtcag gccttctgac
    11701 tgctcccgtg gatctggcag gtcgcatact tcactggctt tcgcgcatcc tgttcccgat
    11761 tgcttttata cctcttctct ttccttatac taccgttcta ttcatcgtgc tggtcttcgc
    11821 cgcggtgatc gtctatctgg gtgaaaggcg atcaggcggc cttccgctca cccaatcaag
    11881 atctttccat gtgatgagac tttccagcat tgcagtgctg ctgaatgcag cccttttctt
    11941 tggccttttc ctgtattcgc agacatgcat ccttcgccct ctttcgcaac agcttgaccc
    12001 gctcagagag tactacagac aacaaggcca gacacaatcg gagacaaaac tatgaacgtt
    12061 gtaatattta cagatacgta tcttcctaaa gtggacggcg ttggaatttc agtggaccac
    12121 ttcacccgaa tcctgggcgg acgaggacac aattttgtaa tatgtgctcc gcgatattca
    12181 gaccacgatc cagaaaccat cagcgatcac attcacgtca ttcgctttaa gaacgcacca
    12241 ctcccttcgt atcctgacat caaagtggtg atcccttcgc ataaaaaact tacgaaggcc
    12301 atgaccatgt tcccaccgga tctggttcac atccagactc cgggcctgct gggccagtac
    12361 ggagtgctag cggccaaaat gtatggagtg ccaatgatag gcacgtatca tactcttgtg
    12421 tcagaacagg acacgtacat ttcgctctat cgacttttga aagtggatac tcttctgaac
    12481 tatttcaagt cgcacaagaa aattgaaaag cgtctggata aaattgaaag aaagaagaaa
    12541 gaaaaaacac tcaaaagcca gctgatctac aagctcactc tcggactcta tcaggcgggc
    12601 gaactcatca tcagcccaag tcatttgatc aagaaggatc ttgtagaagg aggagcttct
    12661 atcccaatcg aagtggtctc caacggaatg gatctcgatc gctttcgcgg cgaaatcaaa
    12721 gagccgcgca aaccaccaaa gtttctgcat gtgggtcgca tttcttttga gaagaactgt
    12781 gaagtggtac taaaagcatt cgccttgatt ctggagaaaa tcccggatgc cacactcgac
    12841 atcatcggag atggcccggc gcttacatcc atcaaaatcg aagccaagca aatcggagtg
    12901 ttcgatcgct gcaatttccc aggatttgtg gcgcacgggt ctttgcatga gatttatcca
    12961 aaatacgatc tcttcctgac ggcaagcaca atggaaacgc aagggcttgt agtcctcgaa
    13021 gcgatggcat gtggactacc gtgcgtagga gtcaatgcat acgctcttcc cgaactcatt
    13081 cagcatgaaa gaaacggctt cgtggtagaa ccgtttgatc acattcacat ggcggaacgc
    13141 gcattgcaac tgttaggcga tcctgaactc tacaagaagt tctcagctca aagcctggcc
    13201 atcgccggag agcatgagct caactcctgc gcagatcgcc tggagagtgt ttaccacagt
    13261 gtagtagaaa aacataggaa aaagcccgca ggactctttg gcggactgct cccgggttga
    13321 ggtgtgatca agcgtcgggc ttttcttttg gccggacgct tacgaactta tctgtaaaag
    13381 gaacttcgat gcgggcaatg gtcccgcgtt cttcccgacc aattcgaaac aatgttgcat
    13441 ccaggttttc acctttgagg agcaaaatgt ttagtgcaaa tccaagtcct tccccttccg
    13501 tctggtctgc atgatccaga aagtattcat gaagggaatc gtacgtcatg gccttctgga
    13561 acttttctcg gatgcgctct tcatcttgcg cgagaatagg acgattgttt atgatttcaa
    13621 tgcggagtcc atcatctgtg tgagtgaaaa tcatttgaac atgcaaaccg agtgagcgcg
    13681 ccttctctgc atatgcttcg agccatgggc gagtcagcac cacgtctttg aatccggcca
    13741 ttcccttttc gtagtcttcc ggtttgtcta tatccaggcc ccgctccgta aaatacacat
    13801 gcttcatgtt tgctttggtt gcattggttg ccagttccag agcgcaggag aagagagcat
    13861 ctttcaaatg agcgcggctg tatctctcca ggatcttttc gaccgtcagg agcagctgtt
    13921 tttccactgc ctcgtccacg cggaaggaaa tgatctctat cttctggccg tcatcgagcg
    13981 ccacttgaag attcttttct atttcaaaat aattcatggt cgtttaacgg ttcggacgag
    14041 ggcgacttgc aacgtaccgg gctattccag ggttttcaag caaaaagcta acgattacgg
    14101 actgcgcgtt ccagaactgc taaaacgttc ttccagctgg ttttggtgac caggaaccga
    14161 ggcaatttga aaggatccgc gtttcttgcc agcggaagaa gaaccggacc ctggacgatt
    14221 gtaaacgaat acttatatcc ggcctttttg atttcatctc gggccagatc agagaaaact
    14281 ccgaacggat aagcaaatac ttccggcctg tgtccgaatc tggactcgag tgtgtctctt
    14341 gaatcgtaaa tttcctttaa gaatcctttc ttgtccttgt tgtactgctt ctcattgaga
    14401 aagagatgat agtaaccatg cgcacccagg gtcgcgcctt cccgcaaata gcggttgagc
    14461 tgatcgttag tcatggcata gggaacgttc gaacgattga tgatgttcgg gtaaatgaaa
    14521 agcaaaggct tgatgtcgtg cttctttaag acacgatcat agatcgcttc cactgattta
    14581 tttccatcat ctatgctcac cagaacgttc tttgcaccgt tgactctgtc tgccagaacg
    14641 tcttctgttc gcacgaagcg aaaacccaga tcgtgaacct gttgcatttg cttttcaaat
    14701 gcctcaggcg ctacgtcgta gtctgtcttt cttccttgaa acgaatggta gcaaagcaca
    14761 tacaatgttc cttctgcgta tgcagaagtg atcaaagcaa aaaaaagaac gaacgcacga
    14821 atcatactta cgtatagctc accctgtaat cgttcatggc aacgcgaatc acctcatgag
    14881 cagtttccac gccatagatc tccgcaatct caacagcgcg agggccggca ccaggaatct
    14941 gtttgtacgt aaggaaatag tgacgaagtc gatcgagcag agtctctgga atgtgtttga
    15001 tgtccgtgca agcatcgaat agagcatctc cgcgcatcac cgcgatgatc ttgtcatctg
    15061 cttccccccg gtcgatcagg cgaagcccgc caatgggacg ggcgcgcagc agaatatcgc
    15121 ttcgcgtgat ggttgcttcc gccagaacac aaatgtccag tgggtctcca tccccttttt
    15181 caatcttctt gttcttcgca cgagcaaggg cagcaattcc gtctccgcaa taagtgcgcg
    15241 ggataaatcc atacggagtg ggacagagac tggagtactg ttgagggcga tcgatgatca
    15301 aatgaccggt ggtcttatcg agttcgaact tcactgtgtc cagcgggaca atttcaatgt
    15361 acgcagtgag aagttctggc gcctgttctc cgggatgaat gccatgccag ggatgcgcaa
    15421 cttcgtgaaa tactctcatg gcgcagattt caacgggcac cagggagggc aaatgattct
    15481 ggaggctttt ttcttgccgg ccaaacgtgc ctgaaaggag ctacgacatg taccaaaaag
    15541 ggaagacctt cgatcaaatc gccgtcggag aaaaggcaag cttcacgaaa actatcacgg
    15601 aaacggacat ttatctatat gcgggaatca gcggcgattt caacccactg cacgtggacg
    15661 aagaatacgc gaagaccaca cccttcaaag gcagaatcgc acacgggggg ctggccggat
    15721 ctctcctggc gcccgtcctt ggtatgaagt tacccgggct aggcacgatg gctctggaag
    15781 taactcaaaa gtatcgtagc ccggttttcc ctggagatac catcacctgt accgtggaag
    15841 tgactgaaaa aatcgcgaga atgaaggctg tggacatgaa aatcctatgg accaaccaga
    15901 aaggagagac gattgcaaag ggaaactgca gagttctacc gcctgcctga ggagacgcag
    15961 agcggtttag cgcttgcgct tttctgcaag cacagttcga tgcggtatga cggaaagaaa
    16021 agaagttgct ctcatcacag gcgcatcgag cggaatcggc caagacatag cacgcatcct
    16081 cgctgaaaag aaaattgatc tgatcatcac tgcacgcagg cgtgatcgcc tggatgctct
    16141 ggccaaagac ctcagaacca tacacaacat tcaagtagac gtcatcgagt ctgatcttgc
    16201 aaaaccagac ggagccgttc aactctacaa tcaggctcgt gccatttcta aagatattac
    16261 aatagttgta aataatgcag gattcggaaa acagaatccc gtcctcgatc agtccataga
    16321 ggaaattcgc tctatgatcc aggtgaacat gacttccttg acggagctca cacgactatt
    16381 cgctgctgac atgaaggccc agggctatgg gcgaattctc cagctcagct ccattggagc
    16441 gttccaacct tcgcccatct atgcagtcta ctcggcagca aaatcgtatg ttctttcttt
    16501 ttcgtatgcg atgaatcacg aactcaaggg aagcggtgtt agcattacta caatgtgtcc
    16561 tgggatcaca gaaactgaat tccacgatgt agccggtcac gaaaagaccg gattcatgaa
    16621 atcgttctct atgacttcgc gcaaagtggc agagatcggc gttcgcgcga tgatgaagcg
    16681 gaaggccaat gtgattcctg gtttctccaa cgcaatcaac agctggctca tggaatctat
    16741 gccgcgcgcc gtttctacca gcattgcggc ctccctgatg aagaaaagct aacgcttgga
    16801 tttgaaaaag ggagtccggc ggaagggcaa atcaacgagc gcaaagacgt tcttcgcaac
    16861 atcgccaatc tggttctgtc cggaaagcgc aaggcagaca taaccagcgt ccagcagcaa
    16921 ctcgatctaa gggaggaact cagagcgagc ttcaaccacg ttgtaggagg cggaatcgag
    16981 gaagtatact ttaacgaatt taccctggat tgatgattcg actgcgtttt tgattgcagt
    17041 ctttgccccc ggacgtcctt tacgcacctg aggcaaccat caatgtccga acttctcatc
    17101 acaaaacata taccagacat gtacgcaaga gcagcaaggc tctttgcgca aagaccagct
    17161 ttcgcaacgc gcgtcaaagg aactacggac tggcagagca tcacctttca ggagttatac
    17221 gaaaccggcc tggatctggc tacggcactc attgaccttg gcgtaaaagc ccgagatcac
    17281 gttgccctcc tggcggacaa tagactcgaa tggatcatcg cggactacgg aattcaactt
    17341 tgcggggcag cagacgttcc tcgcggcaca gatgtcacag acatggatat ccagtacatc
    17401 cttccacact cagaggcgaa ggttgtattt gtggaacatc ttgaaatgct taagaaactg
    17461 gacaagaaca agcgtaagct ctccaaaatc aaacacatca tcctcatgga aaagagtgcg
    17521 aaggcaccaa agaatgtact gagcatgtac gatctaatcg acaaagggcg cgccatgcga
    17581 gccagaggga acaagaaagc ggaagaaaga atcgcaggta tcaaaccaga cgatcttttc
    17641 actttgatct acacttcggg aactacaggg gcacccaagg gagtgatgct gactcacgcg
    17701 aacatttgct ctcagttcaa acatgaaatc gtgccgttaa acgatcatga tcgaattctc
    17761 tccattcttc ccgtatggca catcttcgaa cgaggattcg agatgctcgc catctctcgc
    17821 ggctgctgca cgtactatac aaacgttcgc agcctgggag aggacatcaa gcttgtgaaa
    17881 ccaaccttca tgggttctgc gcccagactc tgggaaagca tctatttgaa gatcatggac
    17941 ggggtaaaat ccgcaagccc tgtaaaaaga ggaatgttta gagcggctta tttctgttcg
    18001 cgcatgttcc agggatcttt atttttcatc actggcaaga aactggatat ggaaggtaga
    18061 aaccctgccg tatctgcgat cacaggaatt ggtcacgcac tgcgagcagt tgtctttgcg
    18121 gtaccatact ttttgctgga tatgatcgta ctaaaaaaga tgagaacggc cacaggaggc
    18181 cacatcaaag gaactgtatc cggtggtggt gctcttccat tgcatgtgga tgaattcttc
    18241 aactatattg gaattccagt actcgaaggt tatgggatga cggaaacagc tcccgtactg
    18301 gcggtgcgca atttcgaaaa aagagtcatt ggaacggttg gtcctgtgtt ccctcaaaca
    18361 gaaattcgca tcattgactt gaactcggga gacgtgctgt ttccaaatcc aaagaatcgc
    18421 gcggatggcc gtggactcaa aggagaaatt cacgcgaagg gcccgcaggt catggcaggc
    18481 tatttcaaga atcctgaggc aaccaaacga gttctgcgcg atggatggat gaacacaggt
    18541 gatattggaa tgatcacgtt caacgattgt gtcaagatta tgggtcgatc caaagacaca
    18601 gttgtgctga tcggcggaga aaacgtagag cccgtcccaa tcgaaaacaa acttgtggaa
    18661 tcccagtaca ttgatcagtg catggtcgta ggtcaggatc aaaaattcct ggcggtcctc
    18721 gtagttcctt ccctcgacgg tttcgcacgt ggcggcctga aagcaaaaac actgtccgaa
    18781 ctcgcttcca atgaagaggc aggcaaagtc atcactgctg aaatcaaacg aatcgtatcc
    18841 gccgaccagg gcttcaagag ctttgaaaga gtgcaggact tcagattcat aaccaagcca
    18901 tttgaagtgg gcgatgagat gaccaacctc ttcaaactga aacgtcatgt gatcacggag
    18961 aagtataaga aagaaatcaa ttcgatgtat aagtagtccg ctcgaagtac aatagttccg
    19021 ttcaaaaata gaccgtttgg aatagaagga ggatctatgt ttggcctgac tcaactcgga
    19081 atcgttcaca cactgattgc gctgatcgca gtcggaactg gcgctttcct tctcatccgg
    19141 gaaggaagaa tttcacccgc cggcctggca ggtaagatct atgtatttgc aactatcgtc
    19201 gcatgcatca ctgctcttgc catctaccag catgggggct ttggaaaggg tcacgtactc
    19261 gccatactca ctctcgtcgt gctcggtgta gcaacggccg ccggcagagg catgtttggc
    19321 ggcgtatcaa aggcagtgga aactgtatcc tattcagcca catttctttt ccactggatt
    19381 ccaggcgttt cagaagcaac aactcgcctt cctctgggtt cgccattgat gaatggcccg
    19441 gaagatccag cgctcaagac gattctcgga gttctcttcg tgctcttcgt agttggatcc
    19501 gttctgcaag tgcgaagcct gcgaaaatcg taacagatcg cgcgaaataa attcgtttag
    19561 tttgacaatg tgaggatgcc cggcgcacac tagtgcaatg tcgggcaatc tgttcctgaa
    19621 cttcttctcg attggagcgc tcattccgct cctcttcgta atgttcatcg cgctcctcct
    19681 cattcgcatc cgcgacaaag gggaatccac gtggtggctt gcttctttcg ccatcgtcta
    19741 ttgccttttt atgcttccgt actttccggc gtccgtcctt tttcatccgg ccggcgccta
    19801 tcacagatgg ttcacggttc cctttgtact acttactttt acattcctgg cccaattctt
    19861 ctttcacatg ccttccaata cgcatccgcg cttcgcaaaa gtctcattct tcgttcaatg
    19921 catcgtggct ctgggagtaa cggtcctctt tgttgcaagg actttccacg ccggcaaagt
    19981 ctacaacttt gacgggcact attttgactt cgatgcagaa gaaataagtc gattggtcgg
    20041 agttttcata atcctttatg tagtttacta tatcatcatc gcggtatttc gcgcagtgat
    20101 tgccaaagac caggaaagaa agatcatcct gtactttctt gcgttcttca tcatcgcaac
    20161 tattccgccc tctgttacaa acatactcag ccgcgacgga gcactggatc gcggcacgca
    20221 ccagacagtc atcgtagcgt gtaacgtact tggattcttt ggagtgatga ccgtgttctt
    20281 gaacacgacc agagatagaa ccacattcat ggccaagatc atttctatca gcatcatcac
    20341 atttctcaca gtattccagg gtttgaatta cttcgtgctc tctgataaag aggaaactta
    20401 cgacgaactg catcgtaagg acgcagccct gatgctcgcc actccttaca gggcagcgga
    20461 tttcagatat gccatggtat ggtccatgaa ggacgcaaaa gaaaaacctg cgttcggaag
    20521 cgtcccggca gtggacctgg acttctacaa aattgaaatc gcaaacgcat ccattctaga
    20581 aag
//
LOCUS       DAORFF010000005        17449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1310_length_17449_cov_4.298494, whole genome shotgun sequence.
ACCESSION   DAORFF010000005 DAORFF010000000
VERSION     DAORFF010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 17449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17449
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1310_length_17449_cov_4.298494"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PKK76_00435"
     CDS             complement(<1..276)
                     /locus_tag="PKK76_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNL66955.1"
                     /translation="MNKFKTSYTIGLLLFAIYCGADKEKGPMIAPIPGASASNHNDLT
                     TAGAFTGTCFTADKFNVGRGIYDITGSAAELGMMGYAMVDQKTAGIHM"
     gene            complement(351..1013)
                     /locus_tag="PKK76_00440"
     CDS             complement(351..1013)
                     /locus_tag="PKK76_00440"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HNL66956.1"
                     /translation="MMPNTTLSRAYYALAIACILDVIAIAAGASIPDFYGRPVLLLSF
                     AVVVFKNAAHNSHRLISLGMVAAALVEAMQMLGIGFAWIASATAVYYLAYSVAFALRG
                     GKIKSKLEATGFAVYALYSIVMFLWLEPYGFLRMASILYTAVVILFGGLSSRPVFARK
                     EGAAWSRLLAGAVALLVISDSMRAISIFKEPVSEALILAPYWAGQFGLALWPIFDKKK
                     QL"
     gene            complement(1074..2354)
                     /locus_tag="PKK76_00445"
     CDS             complement(1074..2354)
                     /locus_tag="PKK76_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL66957.1"
                     /translation="MSLEEAIDDATHFINFEKGLSALSRNPRTGMEQRGNRKWLVLAA
                     FAGTALVSQMLWLNFATIAITIQNRYTVDESTANLLTLVFPLLYLVFSIPSGILIDRK
                     GYRVSVLWGSFMQAAASCIRIYDESFYALLAGQVGIAVAQPFIVNGISKLAADWFDED
                     EGAAANGIGTVGMFAGMALALGLTPVFVEHWGLRTAMIIFSLVSVAAAAIYALIDRKS
                     ISASKESTSSISFGPVLKDKRLQLLFFLAFLGLGFFNGLTAWLEQILAPRGISAIDAG
                     IIGSALIVGGIVGAAIVPNFSDKFKKRKPFLLSCVIAAVCLTYPLGMGSRFSILVTLA
                     AALGFFFLPAFALLLEMCSEVAGEKHTGAATGILMLTGNAGGVVVMLAMDAIRFGSPS
                     YDNAVYLLVVLLAIALFASVFLPETYGAKPIEDK"
     gene            2429..3100
                     /locus_tag="PKK76_00450"
     CDS             2429..3100
                     /locus_tag="PKK76_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66958.1"
                     /translation="MEHSDTQSIAKIDWIVHSLDEEKTHASLWYYGFTGLYATSTVAS
                     FTIASTSHDPIVRATQRVSGWQSLIATGGMLLGPVPSAWAADDIRSMPAGTEAEIKSK
                     LDAAESALRTSAETQSFNRSWINHALGVAVAGGGAAVIRYTYGHKIDKAGGGGRSVRE
                     ATVNFWSSLIVGELQIWTMPVLSMDRWDEYSKKKASESLSIESVRLVLVPANDGLRAG
                     IAMRF"
     gene            complement(3127..3279)
                     /locus_tag="PKK76_00455"
     CDS             complement(3127..3279)
                     /locus_tag="PKK76_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66959.1"
                     /translation="MAFGYLFEGTGAVKKKGSLLGLDAFSGVGNMIMIVCQGCGFVAK
                     SFVIAR"
     gene            complement(3374..4699)
                     /locus_tag="PKK76_00460"
     CDS             complement(3374..4699)
                     /locus_tag="PKK76_00460"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL66960.1"
                     /translation="MRRRNKVPASEKYRPANVARIVYNLVFFAVFVYFKLTVVYPLSV
                     LHLVFAVSWITLIELEILIEKEHWWSGYIPATADLLWISVMVYVTGNISSFVIIGYFI
                     SISLSSMTDSRNYGIYNVILSSVLYAGVGASVYAGLLPAVNILMDPVRPPLLPLVIAS
                     VSMLGSFYIVNQVVSRLFLSLNGEVRTRKKAEDRLRRDLELAHNVQQSIIPAGNRFPV
                     RSEFRFGSCYIALDSVGGDLYDVIPLTGNRFGFLIADVSGHGVSAALVTTMAKASFSN
                     AAPANTLAHEVCATVNRDMFKFIGDSVNYLTAFYCVVDLNTGSVNYCNAGHHPALLVR
                     GSTCQELKSENTFPIGVWAEFDFESGWVSLAPGDRILLFTDGITEASNPAFAQYGESR
                     FQDFIAQSASLNASDFVENLILDLKTFCAGTPIHDDRAVLCIDYLRDSD"
     gene            complement(4750..5088)
                     /locus_tag="PKK76_00465"
     CDS             complement(4750..5088)
                     /locus_tag="PKK76_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL66961.1"
                     /translation="MMTKAEFSGNTLHLSFHVERLDMSMVRALRDEVRGHLVGRPTPV
                     FVDMSNVTYMDSSGMGTLKLIMDDVTAYGGQLVLRKINQPQMMLLKLSKLDSYFQFEE
                     IKKQPGLHDM"
     gene            complement(5117..7000)
                     /locus_tag="PKK76_00470"
     CDS             complement(5117..7000)
                     /locus_tag="PKK76_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNL66962.1"
                     /translation="MYREAAQLFGNLPAFGTRQKDKTWKQISYAELYETGVNLATALI
                     DLGVQQREHVALLADNRVEWIIADYGCQLAGAADVPRGTDVTDADIQYILPHSDAKVC
                     FVEHKEMLSKVQKNLDKCPNIKTLIVMDQGATASGGNVLSMYDLIAKGKELRAKGDKR
                     VEERIESTKPDDLFTLIYTSGTTGAPKGVMLMHSNMISQTQNIPIPITTADRILSILP
                     VWHIFERVFEMMAVSQGACTYYTNIRNIGDDMKNVKPTFMGSAPRLWENIYLKILNNV
                     KGAPPIRRALFNAAYFCSLMFQGSLFFLTGRSLDMEGRNPLVSIAMGILHILRLTLFA
                     IPYFLLDVIVLKKLRGATGGALKGSVSGGGALPLHVDQFFNYIGIPVLEGYGMTETCP
                     VLAVRSWKKLVIGSVGPIFPGTQIRLMDLNTGDVIYPNDKRRAMGRGIKGEIHAKGPQ
                     VMKGYYKNPEATAKVLKDGWMNTGDMGMITFNDCVKIMGRSKDTVVLQNGENVEPVPI
                     ENKMCESVFIDQVMVVGQDQKFLAALVVPSVEEFKKFGIPAETVEELARNPEVLRVIK
                     EEIARLVSGENGFKAFERMQDARVVPKAFEVGDEMTNLFKLKRHIITDKYKSLIDDIY
                     KEK"
     gene            7091..7909
                     /gene="pyrF"
                     /locus_tag="PKK76_00475"
     CDS             7091..7909
                     /gene="pyrF"
                     /locus_tag="PKK76_00475"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNL66963.1"
                     /translation="MSTFYDRFLARRGALRSVLCVGIDPDPAAIPNSDSGIEGTFSFL
                     REIIQATAPYACAYKPNVAFFEVLGPAGWDLFGRIVQCIRREAPEALIVADAKRGDLA
                     NTARFYAETFFKTYNCDGLTVNPYMGMESLAPYDQFSDRGVFALCLTSNPGAIDFQMQ
                     GSPPLFEQVARSVQERNAITRNYWMVAGATRDADSLSRIKSIAPSVPLLIPGVGAQGG
                     ELAVVLRNCGADVLVNASRSILYAYKEHSGVSRGEAAARSAKNVVDEMRSLLSF"
     gene            7913..8797
                     /locus_tag="PKK76_00480"
     CDS             7913..8797
                     /locus_tag="PKK76_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL66964.1"
                     /translation="MLLLIDDNLTICEVVKKLFSGVYDLRITHTAAEGWAALEANAAS
                     VKAILLDWELPDQNGIDFLRKLKQDSRFRLTPVLMLTGRTDEKDIEAGIAAGALYYVT
                     KPFDHRILSALVETALRDFSSAHKTSTVRLSDYESLLTELSFRFSTVSQAGALSQLLA
                     EYCPSPDAARLGLHELLVNAIEHGNLEISHAEKSKLLGEGTLPDEMARRQNVAPYANR
                     SASVRMIRTSEEVRFIIRDQGPGFNYKAFLNLSADRAFAQHGRGIALARSLSFDHLSY
                     VPPGNEVHGRITVPRSAS"
     gene            complement(8841..9167)
                     /locus_tag="PKK76_00485"
     CDS             complement(8841..9167)
                     /locus_tag="PKK76_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66965.1"
                     /translation="MNQKLHHLKESFALLKSMPFPSSIDDALLGDLHAELAEYDGYVV
                     GILETVLSSGQPGKEEVDSAPQLTKALESTQDARVVPLRDYLKQIKACLKAAEEAGLI
                     HFSRGK"
     gene            complement(9186..9506)
                     /locus_tag="PKK76_00490"
     CDS             complement(9186..9506)
                     /locus_tag="PKK76_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66966.1"
                     /translation="MTKQTTFTLLLLVMLSTPALAGPEDQWYRSTFPNGAASLKYHCK
                     KHGNGKPCDQYTRDAISFCKSNLKSARPMTLKDGTPAIKIRRGTVGGYFKENCSQIVT
                     FWYN"
     gene            9594..10154
                     /locus_tag="PKK76_00495"
     CDS             9594..10154
                     /locus_tag="PKK76_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNL66967.1"
                     /translation="MRPSVIASFLLIAAAFTCSRPPVDFREWMLSQMEFNRAATDHLL
                     DELEKTGKADEALAWRAGTNRAPLGWQLMHLAASEDRQATRLGAQSLISEKYAEDFKS
                     GKPAVSFIPKLPYVRQYLKDSRASLQKAIRDFDMSKMDQKVPETDNTFRKLFQIILWH
                     EPHHQGQAHATFNLFKAANGLMDEQK"
     gene            complement(10192..13032)
                     /locus_tag="PKK76_00500"
     CDS             complement(10192..13032)
                     /locus_tag="PKK76_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66968.1"
                     /translation="MSTSESITHQAAAEFFHTSLLSRQSASSRIVMQGGQAAKTIETL
                     HSMLCEMLLKDSRLARTRKLENFPKMSTILADKDGAFQITLGRGLPAESFRASFLGWL
                     AFLRSEGVIEILPALESRGGEIVCNDYLVLQEDKEQDLQLDSFKNESLKTSIDAAGAW
                     LSSFAWSHGVADDLMEKKDPTFVRLLPYYQMLEAMRSDWHAPKDTSQAGFADQIKGKA
                     ELLGKIYNALLGSEKLKFQTKLSGVKKLGDRFPFAWPGSSNAMNDLAAFHDRVRLVFA
                     RDVIMSEKVPGTKPDPKSQFHHANVTAEAFQTAYQSYLKSPDVTPSPYLAHKMGAGLL
                     QQHAHLFSQKEDALRAEPGVLFARNAIVFMLMHSEAVVDHQKEKEKIQFYQAVELYVQ
                     KLASANPPYVDLNVFVRLDKNIVKNENLKKRMIDALRSHQNVKSLEIQDNPTFGHSKL
                     IYLAFHTSQLESMCARSYKAARENKDYNLHMNLERIYDCVQDPSRFQPLVRPDTYERF
                     LQIRQDFFVSQMPFFKRLFWKLFSFARKIDPEQIKDFMKTAMRTEVHKAASIKEKELR
                     TAARKEQERMAKRAVDGSGPHETEPGEIQEHVLDLESTSSSGPSTAPRATSGGRPDPF
                     TEARERSQPDPFSRGATQSRSSERPDFKMPESSTRTEAGRAESSPGVDVSRSSSRSDS
                     TMDRQTTVTRESGSPAGVEVRRENPAASGEPRRAETAAAERRPEERKEERRDEKREEG
                     TRSEEVAGGETGRSSSRLSLEELSAKADRPQVDPDHVQFLKDRPKGAAPQGPQQPQVP
                     EWEIQVIKAGHKVGLDLTERRRLERKKEKEQKKRERHALAVQKLEEHKRKMAAKKKAP
                     RPQQPVPAAAAHAPGTPKHLVIIEVPEKFCAPGKPGRIQFQKKFFKSESFRKEMADFY
                     RKESISASSPEDKKYFAYLISAMEQNYNHYLK"
     gene            complement(13112..15031)
                     /locus_tag="PKK76_00505"
     CDS             complement(13112..15031)
                     /locus_tag="PKK76_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNL66969.1"
                     /translation="MRSHLTQIALFILILDLITRRKVVQDFHSREWMLYVASWSAFLI
                     FLHLSVFFAKLLLREGRRFMYASFLISVSLWLAIGTSVSTVFYFTNGIAPNLFSAEFA
                     LREPYNAWTLLRDTASFAHLMWIVCGAALVFHLLKRATTPAPPTPGDGKAAPDTAFEK
                     TALRTLRKGILPAALSLLFMIMVHNVQFYEQCYVPITGNIVILLRSAWNRATNTQLWS
                     RGFTGRNPIQLSDTHEHANFHLLIILNESLRKKGMQIYGGQRQNTPYMQSLKDSGQII
                     LFSRAYANATSTYLSLPSILTGINPVQETELTLTYPNLFEYAKAAGQHTYYISSHKLS
                     WGRMSVFIKTPALEKYWAYEESGAALSNDLGINDLLTLAEWKKHIRETIQNGETMDHQ
                     RTAGMLHFNTTHYPYNVEPEFSLYSGTSRDSYDNTIYKTDALVQEVFKQLTESAMIDR
                     TIVVFASDHGEAFGEHECVAHFYCQYLETVQVPLWIYVPKALQNKLDMQQLKKTAALN
                     VSNVDLIPTIIGLYGMDSSSQISSLKKRFLGSDLFKAVKPDRTILITTSNEMMRTDVG
                     ASIIQNNQQYLLKLARPAHEELYSFDIDPEENHSMWETTEPEARQTWHKWMDSQKATF
                     EIRQRFRKATEHSTK"
     gene            15148..15675
                     /locus_tag="PKK76_00510"
     CDS             15148..15675
                     /locus_tag="PKK76_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66970.1"
                     /translation="MKLFFAFAIFALMRCIPENQIRSYGDPPAAADSFVYATFDIDSN
                     AGKCLLKLHPTDNKGPDQLFAASLLGLAILTVPPGEYELNEVVFATNLQKKEQPFTSP
                     LYLQKFAVQPGEGVYLGNFHCSAVTHGTPQYNQLRIEFGFKLSKYQMNETTRRIVELY
                     GPQLNGRVFRNLENP"
     gene            15672..16400
                     /locus_tag="PKK76_00515"
     CDS             15672..16400
                     /locus_tag="PKK76_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66971.1"
                     /translation="MKLFNIAILIIITTNHCMLMIRTESPQAIYRGARAGVPTKVRCE
                     IVARTSQIYEHPLDNPMTPGQIEMMVRRGLDQQGYSCVNNDSGLPVLTVDAAVYKTHP
                     NPLFFGLTVFLLDLVPTYFGRLTVDVAVSLDIANKPHRFQYTGNYSIWTSMLFIPTGN
                     PEDYGKKKVREIIEDDMAAFAMDAAFVTGAKSEDCFDSDLVLKDGRRIYSARVSRSST
                     AVDVTARDGRHWVFAPEEIVKVDR"
     gene            16515..17147
                     /locus_tag="PKK76_00520"
     CDS             16515..17147
                     /locus_tag="PKK76_00520"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNL66972.1"
                     /translation="MKIIASLLFLLGTVNCTYEANCSASDMRCNPILSTGLFIRTCYT
                     QGFCKIFATSGTWFGDLGGSTGVSGADRLCNADANRPAGGGFYAALISDGITRIATVT
                     SNIGDGQVGWVMYPNMEYRLANGSTVWTTNSAGLFVFGTVTNQIAAAGTYYTGMDAFW
                     VSSATCLSWTDGTGGNSGRYGTGGASGNTVISLSQSACNSAMRGLYCVQR"
     gene            complement(17193..>17449)
                     /locus_tag="PKK76_00525"
     CDS             complement(17193..>17449)
                     /locus_tag="PKK76_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66973.1"
                     /translation="IYLTPRSSAVVLEFDPVTHTQTTFGSGWGAAVTAFDGGILAPNG
                     KIYAFPLTGSGNNQQPLEIDPKANGNYCPAVARSAYFNKF"
BASE COUNT         4146 a         4247 c         4476 g         4580 t
ORIGIN      
        1 catgtggatt ccagctgttt tttgatctac catggcatag cccatcatgc cgagttcggc
       61 cgcagatcca gtgatgtcgt agattcccct tccaacgttg aacttatctg cagtgaaaca
      121 ggtaccggta aaggccccgg ccgtggtcag atcattgtgg ttggatgcac tcgctcccgg
      181 aatcggagcg atcatgggcc ccttctcttt gtctgcaccg caatagatgg cgaagagcaa
      241 cagcccgatt gtgtagcttg tcttgaactt gttcacagta aattccccct gctatgttct
      301 cagcataaac gcgaaccttg cgattcgctt actttcccct tatatgcccc ttataactgc
      361 ttttttttat cgaaaatagg ccagagtgcg aggccaaatt gtcctgccca gtagggcgcc
      421 agaatcagag cttcagacac aggctctttg aagatagaaa tggcccgcat gctgtctgaa
      481 atcacaagaa gagccaccgc gccggcgagc agtctggacc acgcggctcc ttcctttctt
      541 gcgaagaccg gacgcgaaga gagaccgccg aagaggatta cgactgcagt gtagagaatg
      601 gaggccatgc gaagaaatcc atacggttcg agccacagaa acatgacgat ggagtagagg
      661 gcgtagactg caaatccggt ggcttccagt ttacttttga tcttaccacc tcgcaaagcg
      721 aaagcaacgg aatacgcgag gtaataaacg gcagttgcag atgcaatcca ggcaaatcca
      781 atgccgagca tttgcattgc ttctaccaga gccgcggcca ccattccaag cgaaatcagc
      841 ctgtgagagt tgtgtgccgc gttcttgaag actaccactg cgaatgacag gagcagcaca
      901 gggcgaccat aaaaatccgg aatagatgcg cctgcagcta tggcgatcac atccaaaatg
      961 catgcgatgg ccagtgcata gtacgcgcga gaaagtgtgg tgtttggcat cataaaaccc
     1021 cgtttgccgg ggccttacgc tatttgagtt ctaaatattt gcaaccaaaa atatcattta
     1081 tcttcaatgg gtttcgcgcc gtaggtttcc ggaagaaaca ccgacgcgaa gagcgcgatc
     1141 gcaagaagaa ccacaagcaa gtatactgca ttgtcgtaag agggtgaacc aaagcgaatt
     1201 gcatccatcg cgagcattac aaccactcct cctgcgttcc cggtgagcat gagaatgccg
     1261 gtggccgcac ccgtatgctt ttcgcctgcc acttcggaac acatctcaag caaaagcgcg
     1321 aaagcaggta aaaagaagaa accgagtgct gccgccagag tcacgagaat gctgaaccga
     1381 cttcccattc cgagaggata cgtgaggcag acagctgcga tgacgcacga aagcaggaat
     1441 ggcttacgct tcttgaactt atctgaaaaa ttggggacaa tcgccgcgcc aacgatccca
     1501 cccacaatca aggcacttcc aatgattccg gcgtcaatgg cactgatccc tctgggtgct
     1561 agaatttgtt ccagccatgc agtgagaccg ttgaagaagc ccagtcccag gaacgccagg
     1621 aagaaaagaa gttgcaggcg tttgtctttg agaactggcc caaaagaaat actcgaagtg
     1681 gattctttag atgcggaaat ggacttcctg tctatcaatg catagatagc tgccgctgcc
     1741 acggagacca gagaaaagat gatcatggcg gttctcagtc cccagtgttc tacaaagacg
     1801 ggagtcagtc cgagtgcaag agccattcct gcgaacattc cgactgttcc tattccgttg
     1861 gccgccgctc cttcatcttc gtcaaaccag tccgccgcca gtttggaaat tccgtttacg
     1921 atgaaaggct gagctaccgc aattccaacc tggcctgcga gcaaagcgta gaaggattcg
     1981 tcgtagatgc gaatgcagga agcggccgcc tgcatgaagg agccccacag aacagaaaca
     2041 cgatatcctt tgcgatcgat cagaatgcct gacggaatgg agaaaaccag gtacagaagg
     2101 ggaaaaacga gagtcagaag atttgctgta gattcgtcta cggtgtaacg attttgaatt
     2161 gtgattgcga tggttgcaaa attcaaccat agcatctggc tgacgagagc tgttccggca
     2221 aatgccgcga gcaccagcca ctttctgttt ccgcgttgtt ccatgcccgt tcgtgggtta
     2281 cgactgagtg cactcaatcc tttttcaaaa tttataaaat gtgtggcgtc atcgattgct
     2341 tcttctagag acaatcatga ctttgttccg ctggatttca ctcgttttgt taacggtttg
     2401 tttggcgcac ccgtttcacg ccgaacctgt ggagcattca gatacacaga gcatcgcgaa
     2461 gatcgattgg attgtacact cgctggatga agagaagact cacgcttcgc tctggtacta
     2521 cggttttact ggattgtatg caacaagtac tgtggccagc ttcacaatcg cgtctacttc
     2581 acacgatccc attgttcgcg ccacgcagag ggtcagcggg tggcagtctt tgattgcgac
     2641 gggaggtatg ctgcttggtc ctgttccttc tgcgtgggca gcagatgaca ttcgcagtat
     2701 gcccgcagga actgaggcgg agatcaaaag caagctcgac gcggcagaaa gcgcgcttcg
     2761 aacgagtgcg gagactcaat cattcaatcg gagttggatc aatcacgctc ttggagtggc
     2821 tgtagcggga ggtggcgccg ctgtgattcg ttatacctat gggcacaaaa tcgacaaagc
     2881 aggaggcggt ggaagatctg taagggaagc caccgtgaat ttctggagca gtctgattgt
     2941 aggtgagctg cagatctgga ccatgccggt tctctccatg gatagatggg atgagtattc
     3001 gaagaaaaaa gcctccgagt ctctttccat agagagtgtg cgactggttc ttgttcccgc
     3061 aaacgatggt cttcgcgctg gaattgcgat gcgcttctag agattgcgta agtattcttt
     3121 gaaaactcag cgggcgatca caaagctctt tgcaacaaat ccgcatccct ggcacacaat
     3181 catgatcatg ttgccaacgc ctgaaaacgc atcgagcccg agtagcgatc ctttcttctt
     3241 cactgctccg gttccttcaa acagatagcc aaaggccatt tcctttccgc cacattcaga
     3301 gcacgtcgta ttctttctta tgttttcgaa tttctcttcc atatcatgag tgacggcata
     3361 tgtttattgt gcgtcaatct gaatcgcgaa gatagtcgat gcacagaacg gcacgatcat
     3421 cgtgaatggg cgtgcccgcg caaaaagttt ttaaatcgag gatcagattc tcaacaaaat
     3481 cagaagcatt caatgaagcg gattgggcaa taaaatcttg aaacctggat tctccgtatt
     3541 gagcgaaagc aggattggat gcctcagtaa tgccatctgt gaagagcaag atgcgatctc
     3601 caggcgcaag agaaacccag cctgattcga aatcgaattc tgcccacact ccgatcggaa
     3661 acgtgttttc agacttcagt tcctggcagg tggaacctcg aacaagcagt gctggatgat
     3721 gtccggcatt gcagtagttg acgctgccag tgttgagatc tacaacacaa tagaacgcag
     3781 tcaggtagtt tacgctgtct ccaatgaatt tgaacatgtc cctgttgact gtcgcgcaaa
     3841 cctcgtgtgc cagtgtattt gcaggagccg cgttgctgaa tgatgctttg gccatggtgg
     3901 tgacgagcgc cgccgaaaca ccgtgaccag aaacatctgc tatgagaaat ccaaatcgat
     3961 tcccggtgag aggaatcaca tcatacagat ctccaccaac actatcgagt gcaatgtagc
     4021 aggatccgaa tctgaattcg gatcgaaccg gaaagcgatt tcctgctggt atgatgcttt
     4081 gctgaacgtt atgcgccaat tcgaggtctc gcctgagtct gtcttctgct ttctttcgcg
     4141 tgcggacctc tccattgagc gaaagaaaaa gtcgcgatac tacctggttt acgatataga
     4201 aagagcccag catggagacg gaagcaatca caagcggcaa gagcggcggt cgaacaggat
     4261 ccatcaaaat gttgactgca ggtagaaggc cagcgtatac agacgcgcca accccggcgt
     4321 acaaaacgct gctcagtatt acattgtaga tgccataatt gcgcgaatcg gtcatgctgg
     4381 agagcgaaat ggaaataaaa tagccaatga ttacaaatga ggaaatattt ccggtcacgt
     4441 acaccatcac gctgatccag agtaggtccg cagtggccgg aatatagccg ctccaccagt
     4501 gttctttttc gatcagaatt tctagttcaa tcagcgtgat ccaggaaact gcgaagacca
     4561 gatgtaaaac ggaaagtggg taaacgactg taagcttgaa gtagacaaag actgcgaaga
     4621 ataccaggtt atagacgatg cgagctacat tggccgggcg gtatttctcg gacgctggta
     4681 ctttgttccg ccgccgcacg atgctggagt gtgatggaaa cggaagaagc tgtcaagccc
     4741 taacggcgat tacatgtcgt gaaggccagg ctgcttcttg atttcttcaa attggaagta
     4801 cgaatccagt ttggaaagtt tcaacagcat catttgaggc tggttgatct ttcgcaacac
     4861 tagctggccg ccgtacgctg taacgtcatc catgatgagc ttgagcgtgc ccataccgga
     4921 agagtccatg tacgtaacat tggacatgtc tacaaatacg ggagtcggtc ttcccacgag
     4981 gtggccgcgc acctcatcgc gcaacgcccg gaccatgctc atgtccaggc gttccacgtg
     5041 aaaggatagg tggagagtgt tgccggagaa ttcagccttt gtcatcattg ggcctggttc
     5101 tccgtcttca ttcttctcac ttttccttgt aaatgtcgtc gatgagcgac ttgtatttgt
     5161 ccgtgatgat gtgtctcttc agtttgaaga ggttggtcat ttcatcaccg acttcgaaag
     5221 cctttgggac cacacgtgcg tcctgcatgc gttcaaatgc tttgaaaccg ttttcacctg
     5281 agaccagtct tgcgatttct tctttgatta cgcgaaggac ttctggattc ctcgcaagtt
     5341 cttctactgt ttccgcaggg attccgaatt tcttaaattc ttctaccgac ggaacgacga
     5401 gcgcggccag gaacttctga tcctgaccta caaccatcac ctggtcgatg aacacagatt
     5461 cgcacatttt gttttcaatt gggactggct ccacgttttc accgttttgc agaaccacag
     5521 tgtccttcga gcgacccatg atcttcacgc aatcgttgaa ggtgatcatt cccatgtcac
     5581 cggtgttcat ccagccgtct ttgagtacct tggcggtagc ttccgggttc ttgtagtagc
     5641 ctttcattac ttgcgggcct ttggcgtgaa tttcgccttt gattccgcgg cccatagctc
     5701 tacgcttgtc gtttggataa atcacatctc cagtgttcag atccatcaag cgaatctggg
     5761 tgccagggaa aataggtcca acgctaccga tcaccagttt cttccacgaa cgaacggcga
     5821 gaacaggaca agtctctgtc atgccatagc cttcgaggac tggaattcca atgtagttga
     5881 agaactgatc cacatgaaga ggaagtgctc caccaccgga tacagaaccc ttgagcgcgc
     5941 cgccagttgc tccgcgaagc ttcttcaaga cgatgacgtc gaggaggaag tacggaattg
     6001 caaagagcgt aaggcgaaga atgtgcagaa tccccattgc gatggaaacc agcgggtttc
     6061 ttccttccat atcaagagat cttcctgtga ggaagaacaa ggagccctgg aacatcaatg
     6121 aacagaagta tgccgcattg aagagcgcgc gacgaattgg aggtgcaccc tttacgttgt
     6181 tcaagatttt caggtagatg ttttcccaga gacgcggggc agatcccatg aacgttggtt
     6241 taacgttctt catgtcatcg ccaatgttcc ggatgtttgt atagtaggta caggcaccct
     6301 gacttaccgc catcatttcg aaaacacgct cgaagatatg ccatactggg agaatggata
     6361 gaatcctgtc cgcagttgta attgggatcg ggatgttctg agtctgcgaa atcatgttcg
     6421 agtgcatgag cattacgccc tttggcgcac ccgtagttcc agatgtgtag atcagcgtga
     6481 acagatcgtc tggctttgta ctttcaatcc gctcttctac tcgcttgtct cctttcgcgc
     6541 gaagctcttt tccttttgcg atgaggtcat acatactcaa aacgttgccg ccggacgcag
     6601 ttgctccctg gtccatgaca atgagtgttt tgatgtttgg gcatttatct agattcttct
     6661 gaactttgga caacatctcc ttatgttcta caaagcacac tttcgcatct gaatgcggaa
     6721 gaatatactg aatatcagcg tctgttacgt ctgttccgcg aggaacgtca gctgctccgg
     6781 ccagctggca accgtaatcg gcgatgatcc attccacgcg gttgtccgcg aggagggcaa
     6841 cgtgttcgcg ctgctgaact cccagatcaa tgagcgctgt cgcaagattt acgccagttt
     6901 catataactc tgcgtagctg atttgtttcc atgtcttgtc tttttgtctt gtcccgaaag
     6961 ctggaagatt tccgaacaac tgagccgcct cgcgatacat cgcaggcagt gttttcgcag
     7021 taatccttgt tggcacgtga gtcctccccc cgaaaagtag gctcagaaaa agaattgtac
     7081 ggaagtctgg atgtcaacct tttatgatcg ttttttagcc cgcaggggtg cattgcgctc
     7141 ggttctttgt gttggaatcg atccggatcc cgcagctatt ccaaattctg attcaggtat
     7201 tgagggaacg ttttcttttc tgagagagat cattcaagct actgcaccct atgcgtgtgc
     7261 atacaagccg aacgttgcat tctttgaggt tctgggtccg gctggctggg atttgttcgg
     7321 tagaattgtg cagtgcattc gaagagaggc gccggaggcg ctgattgtgg cggatgcgaa
     7381 gagaggagac ctcgcaaata cggcacgctt ctacgcggag acatttttca agacttacaa
     7441 ttgcgacggg ctcacggtca atccgtacat gggaatggag tcgcttgcac catacgatca
     7501 gttttcagat cgcggagtct tcgcattatg tctcacatca aatcctggtg caattgactt
     7561 tcaaatgcag gggtcacctc cactttttga acaagtcgcg cgatctgtac aggagagaaa
     7621 cgcaatcact cgcaactact ggatggtggc aggagcgacg agagatgcgg attctctttc
     7681 tcgtatcaaa tcgattgctc cgtccgttcc acttctgatt cctggagttg gtgcacaggg
     7741 cggtgagctc gcagtggtcc ttcgcaattg cggcgcagat gttcttgtga atgcgagccg
     7801 gtccattctc tacgcataca aggaacattc aggtgtctcg agaggtgagg cggctgctcg
     7861 ttcggccaaa aacgtggtcg acgaaatgag atcgcttctt tccttttaag ctgtgcttct
     7921 gctcatcgat gacaatctga cgatttgcga agtcgtcaaa aagctcttct cgggcgtata
     7981 tgatctgcgc attacgcaca cggctgcgga aggctgggca gccctggaag caaacgcagc
     8041 aagcgtcaag gcgattctcc tggactggga acttccggat caaaatggta tagatttttt
     8101 aagaaaatta aaacaggatt ctcgtttcag gctgacccct gttctgatgc tgaccggccg
     8161 cacggacgaa aaggacatag aagcagggat tgcggccggg gcgctttatt atgttacaaa
     8221 gccgtttgat catcgcattc tcagcgcgct ggtggaaacc gcgctccgag attttagttc
     8281 tgctcataaa accagcaccg ttcggctttc agactatgag tcgcttctaa cggaactgtc
     8341 gtttcgattc agcacagtga gccaggcggg cgcgttgtct cagctcttag ctgagtattg
     8401 tccttcaccc gatgcagcga ggctcggtct acatgagtta ctcgtgaatg cgattgagca
     8461 cgggaatcta gaaatctccc acgctgaaaa atctaagttg ctgggagaag gaactttgcc
     8521 tgacgaaatg gcgaggaggc agaacgtcgc accctatgca aacaggtctg ccagcgttcg
     8581 gatgattcgc acaagcgaag aagtacgctt catcattcga gatcagggac cgggctttaa
     8641 ttataaagca ttcctcaatt tgtcagcgga cagagctttt gcacagcacg gtcgcggcat
     8701 tgccctggct cgttctttgt cgtttgatca tctttcttat gtgcctcccg gaaacgaggt
     8761 tcatggtcgc ataacggttc cgcgaagtgc ttcgtgaagt cttgccgggc gttgatgtct
     8821 ccgagcagcg gctcagtttt ctatttcccc cgcgaaaagt ggatcaggcc tgcttcctcc
     8881 gctgctttca ggcaggcctt gatttgtttt agatagtcgc gaagcgggac gactcgcgca
     8941 tcctgtgtgg attccaatgc tttggtaagc tgaggagcag agtccacttc ttctttcccg
     9001 ggttgccctg aggaaaggac ggtttccagg atgcccacga catagccgtc atactccgcc
     9061 agttccgcat gaagatctcc gagcaatgca tcatcgatgc tggatggaaa tggcatcgac
     9121 ttgaggagag caaatgattc tttgagatga tgtaactttt gattcatggc gacagttgat
     9181 ctaaactaat tgtaccagaa ggtaacaatc tggctgcagt tttctttgaa gtatccgcct
     9241 accgttccgc ggcgaatctt gatggccgga gttccgtctt tgagagtcat gggccgcgca
     9301 cttttcaaat tggatttgca gaatgaaatt gcgtcgcgcg tatactgatc acacggcttt
     9361 ccgtttccgt gttttttgca gtgatatttg agactggcag caccattcgg aaaagtggat
     9421 ctgtaccatt ggtcctccgg ccctgcgagc gctggagtgc ttagcatcac cagaagtaga
     9481 agagtgaagg ttgtttgctt tgtcacgata cccgggcaaa aagaactcta aaacgattgg
     9541 gtgcaatcag aaaattcata gcctgaagcc agctcattgt tttcgtgttt tccatgcgcc
     9601 catctgtcat cgcttctttc cttttgatcg ccgcggcatt cacttgctcc agaccacccg
     9661 tcgattttag agagtggatg ctttcgcaga tggaattcaa tcgagcggcc acggatcatc
     9721 ttctcgatga acttgaaaag actggcaaag cagatgaagc tctcgcctgg cgtgcaggga
     9781 ccaaccgggc acctctcggg tggcaattga tgcacctggc cgcgtccgag gatagacagg
     9841 ccactcgcct gggggcccag tctcttatat ctgaaaagta tgcggaggat tttaagagcg
     9901 gcaaaccagc cgtgtcgttc attccgaagc tgccctacgt gcgccagtat ttgaaagatt
     9961 cgcgtgcgtc tctccaaaaa gcaattcgtg actttgatat gagcaaaatg gatcagaaag
    10021 ttccggaaac agacaataca tttcgtaaac tatttcaaat tatcctgtgg catgagccgc
    10081 atcatcaggg ccaggcccat gcgacgttca atctatttaa ggccgccaac ggtttgatgg
    10141 atgagcaaaa ataaatatgc tggcacaatg agttgcattt gagcgcgcca ctcacttcag
    10201 gtaatgattg tagttttgtt ccatggcgct aattaagtag gcaaagtact tcttatcttc
    10261 gggagaggaa gccgagatgg attctttgcg ataaaaatcc gccatctctt tgcggaaact
    10321 ttcgctctta aaaaactttt tctgaaattg gattctaccc ggtttgccgg gcgcacaaaa
    10381 cttttcggga acttctatga tcacaaggtg tttcggagta ccaggtgcgt gagctgctgc
    10441 tgccggtaca ggttgctgcg gcctgggagc ctttttcttc gcggccattt tccttttgtg
    10501 ttcttctagc ttctgcacgg cgagcgcatg tctttcgcgt ttcttctgtt ctttttcctt
    10561 ttttctttcg agtcgccttc tttccgtgag atccagacca accttgtggc cagctttgat
    10621 cacctggatt tcccactccg gtacctgagg ttgctgcggg ccctgaggcg cggccccttt
    10681 aggacgatct ttcaggaatt gaacgtgatc cggatctacc tgcgggcgat ctgcttttgc
    10741 ggatagttct tccaggctga gcctgctcga cgatctcccc gtttctccgc cggcgacttc
    10801 ttcgctcctg gttccttctt ctcgcttctc atcccggcgt tcttcttttc tctcttccgg
    10861 tcttctctct gctgcggctg tttccgcccg tctgggctct cctgatgcag ccggattttc
    10921 gcgtctgact tccacgcctg ccggagaacc tgactctcgt gtaaccgttg tttggcgatc
    10981 cattgtagaa tcagaacgtg atgaggagcg acttacatcc actccaggac tcgattctgc
    11041 tctgcccgct tctgtccttg tgcttgactc aggcatttta aagtctggcc tttccgatga
    11101 ccggctctgt gtcgcgcctc ttgagaaagg atccggctgg ctacgctcac gcgcctccgt
    11161 gaaaggatca ggacgtccgc cggatgttgc tctaggagca gtagaaggtc ctgaagatga
    11221 ggttgactca agatccagaa cgtgttcctg gatttctcct ggctccgttt catgtggtcc
    11281 ggagccatcc acggcgcgtt tggccatccg ttcctgttct ttgcgcgctg cggtacgaag
    11341 ttccttttct ttgatactgg ctgctttgtg aacttctgtt cgcattgcag tcttcatgaa
    11401 atccttgatc tgctccggat ctatttttcg tgcgaagcta aagagtttcc agaagagcct
    11461 tttgaagaaa ggcatctgtg atacaaaaaa gtcctgtctt atttgaagaa agcgctcgta
    11521 tgtgtccggt cgaaccagtg gctggaaacg agaaggatcc tggacgcaat cgtagatccg
    11581 ctccaggttc atgtgtaagt tgtaatcctt gttttctcgg gctgctttat atgagcgagc
    11641 gcacatgctt tccagctggc ttgtgtgaaa ggctagatag attagtttag aatgtccgaa
    11701 agttgggtta tcttgaattt ccaggctctt tacgttttga tgcgaacgaa gagcatcgat
    11761 cattctcttt ttcaggtttt cattttttac aatgttctta tccaatcgaa caaatacatt
    11821 caggtccacg taaggtggat tggctgacgc cagtttctgc acgtacagct ctaccgcctg
    11881 atagaattgg atcttttctt tttctttttg atgatctaca actgcttcgg agtgcatcag
    11941 catgaataca attgcattgc gagcaaatag tacgcctggt tctgcgcgaa gtgcatcttc
    12001 tttctgcgaa aagaggtgag catgctgctg aagcagaccg gctcccatct tgtgcgcaag
    12061 ataaggagaa ggagtgacat ccggcgattt taaatagctc tgataggcag tctggaatgc
    12121 ctccgctgta acattcgcat gatgaaactg agatttcgga tctggttttg tcccgggcac
    12181 tttctcagac atgatcacat cgcgtgcgaa aacaagacgg actctatcat gaaaggccgc
    12241 cagatcgttc attgcattcg atgaaccggg ccaggcaaat ggaaaccgat cccccagctt
    12301 ctttacaccg gacaatttgg tttgaaattt tagtttttct gatccaagaa gtgcattata
    12361 aattttccca agcaattctg ccttgccttt gatttgatct gcgaaacctg cctggcttgt
    12421 atctttgggc gcatgccagt ctgatcgcat ggcttcgagc atctgatagt aagggaggag
    12481 ccgcacaaac gttggatcct ttttttccat caggtcatcc gcgactccgt gactccaggc
    12541 gaatgaactc aaccatgcgc ccgccgcgtc gatagaggtt ttcaatgatt catttttgaa
    12601 gctatcaagt tgcagatctt gttccttgtc ttcctggaga actagatagt cgttgcagac
    12661 gatttctccg cctctgcttt cgagagcggg tagaatttca atgacacctt ctgagcgcaa
    12721 gaatgcaagc cacccaagaa agctggctcg aaaactttct gcgggaaggc cgcggcccag
    12781 cgtgatttga aacgctccat ccttgtccgc aaggatcgta gacatctttg ggaagttctc
    12841 cagctttcta gtgcgagcga ggcgtgaatc tttgagaagc atttcacata gcatgctgtg
    12901 cagagtctcg atggttttgg ccgcctgccc accttgcatt acaatgcgac tactggccga
    12961 ctgtctggat agaaggctgg tgtggaaaaa ttccgctgct gcctggtgtg tgatgctttc
    13021 tgatgtagac atgaacggcc cggaaatgac atgaaaagaa gtagaatctt tcgtcaagga
    13081 ttgttgagat ttgatcctgt tttcagtgtg attactttgt agaatgttcg gttgcttttc
    13141 taaaacgctg cctgatttca aaggttgctt tttgagaatc catccatttg tgccaggttt
    13201 gtcttgcttc tggttccgtg gtttcccaca tggaatggtt ttcttccggg tctatgtcga
    13261 atgaatagag ttcttcatgt gcagggcggg ccagtttaag caaatattgt tgattgttct
    13321 ggatgatgct tgctcccaca tccgtacgca tcatttcatt ggaggtcgta atcaggatcg
    13381 ttcgatcggg cttaactgcc ttgaagagat cgctgccaag gaatcttttc ttgagggagg
    13441 agatttgcga gcttgagtcc attccgtaaa ggccgattat cgttggaatg agatccacgt
    13501 tcgaaacgtt caatgcggct gttttcttca gttgctgcat atccagtttg ttttgaagtg
    13561 ctttcggaac atagatccag agcgggacct gcaccgtttc cagatactgg caatagaaat
    13621 gagccacgca ttcatgttcg ccaaacgcct caccgtgatc ggatgcaaat actacaatcg
    13681 tgcggtcgat cattgcagat tccgttagtt gcttaaagac ttcttggacc agtgcgtctg
    13741 tcttataaat cgtattatca taggaatcac gactcgtgcc agaatagagg ctgaattccg
    13801 gctccacatt gtacgggtag tgagtggtat taaaatgaag catccccgcg gtcctttgat
    13861 gatccatggt ttctccgttc tgaatcgttt cccggatgtg ttttttccat tctgcgagag
    13921 tcaatagatc gttgattcca aggtcgttgg aaagagcggc accactttct tcataggccc
    13981 agtatttctc cagtgcgggc gtctttatga atacggacat tcgaccccag ctgagcttgt
    14041 gcgatgatat gtagtacgta tgttgtcccg ccgccttcgc atattcgaag agattgggat
    14101 aggtgagggt gagctctgtt tcttgcaccg gattaattcc ggtgaggatg ctcggaagag
    14161 acagatacgt ggatgtggcg tttgcatacg ctcgcgaaaa cagaatgatc tgaccgctat
    14221 ccttcagcga ttgcatatac ggtgtgttct gcctttgtcc gccataaatt tgcatgcctt
    14281 tcttacgaag gctttcgttc agaatgatga gaagatgaaa gtttgcgtgt tcgtgtgtat
    14341 cgctcagttg aattggattt cttccagtga agccacggct ccagagttgt gtgttcgtgg
    14401 ctcgattcca cgcggatcgg agtaggatta cgatgtttcc cgttatggga acatagcact
    14461 gttcgtagaa ttgaacgtta tgcaccatga tcatgaagag caaagatagt gccgccggga
    14521 ggatgccttt tcgcaatgtt cgaagcgcgg tcttttcaaa ggcagtatcc ggggctgcct
    14581 ttccgtcacc gggtgttgga ggggcaggtg ttgtggcgcg tttgagcaag tggaacacca
    14641 gggccgcgcc gcaaacgatc cacatcaggt gggcgaacga ggctgtatcg cgaagcaaag
    14701 tccaggcatt gtaaggttct cgaagtgcga actccgccga aaagagattg ggcgcgattc
    14761 cgttcgtgaa gtagaagacc gtggaaacag acgttccaat ggccaaccat agggagacgc
    14821 taataagaaa agaggcgtac ataaatctcc gtccttcgcg taacagtagt ttggcgaaaa
    14881 aaacggacag gtgtaagaag atgagaaacg cagaccatga tgcgacgtac agcatccatt
    14941 cacgactgtg aaaatcctgg actactttcc tgcgcgtaat gagatcgagg atcaatatga
    15001 aaagcgcaat ctgcgtcaga tgagaccgca tgattcttct aaataatgtc acgatggcac
    15061 tacttgtgaa atgcgttcgc cggtcaccca attcgcgtgt gagcgaaaat gattgacaga
    15121 agttttttct aatccccagt tgcgcgcatg aagctgttct ttgctttcgc cattttcgct
    15181 ctcatgcgat gcattcccga gaaccaaatt agaagctacg gggatccgcc ggcggcggca
    15241 gatagtttcg tatatgcgac gtttgatata gactccaatg ccggaaaatg tctgctaaag
    15301 ctccacccga cagacaacaa ggggccggat caactgttcg cagcgagtct actcggcctg
    15361 gcaattctta ctgtgccccc cggcgagtat gaactcaatg aagtggtttt tgcaactaat
    15421 ttgcaaaaga aagagcagcc gtttacgtcg ccactctatc ttcagaaatt cgcagttcaa
    15481 ccaggggagg gcgtctacct gggtaatttt cattgttcag ctgttaccca cggcacgcca
    15541 caatacaacc agctccggat tgagtttggg ttcaaactga gcaagtacca gatgaatgag
    15601 acaactcgaa gaattgttga actctacggt ccgcaactca acggccgcgt ttttagaaat
    15661 ctggagaacc catgaagctc tttaacattg cgattttgat aatcattaca acaaatcact
    15721 gcatgctcat gatccgaaca gaatcgccgc aggcgatcta taggggtgcc agggctggag
    15781 ttcctacaaa agttcggtgt gagatcgtgg ctcgcacatc gcagatctat gaacacccgc
    15841 tcgataatcc aatgacaccc ggtcaaattg agatgatggt tcgccgtggt ttggaccaac
    15901 aaggttacag ctgtgtcaac aacgactccg gtttgcctgt tctcaccgtc gatgccgcgg
    15961 tctataaaac gcatccgaat ccgctatttt tcgggctgac ggttttcctt ctcgatttag
    16021 ttcccaccta ctttggcagg ctgactgtcg acgtcgccgt ttcactcgat atagcaaaca
    16081 agcctcatcg ctttcaatat acgggcaact attctatctg gacttctatg ctctttatcc
    16141 cgaccggcaa tcccgaagac tatggtaaga aaaaggtgcg cgaaatcatc gaggacgaca
    16201 tggctgcttt tgcaatggac gctgcgtttg tgacgggagc gaaatcggaa gattgctttg
    16261 actcggacct ggtactcaag gacggcagac gcatttactc cgcacgcgtt tcacggtctt
    16321 cgacagctgt tgatgttact gcacgcgacg gacggcattg ggttttcgcg cctgaagaaa
    16381 tcgtgaaggt ggatcgatga atgttgtgga ccgattgatg ttcgggtcgc atgaaagtcc
    16441 gcgtgtgctc acaatgcgct cgacaaaccg tcgagtcttc gccaggatac ctccgggaca
    16501 cgatgatgtt tcggatgaag ataatcgcat cactcctttt cctgctggga acggtgaatt
    16561 gtacgtatga agcgaattgc tcggctagcg acatgcgatg caatcccata ctttcgactg
    16621 gcctgttcat acgaacgtgt tacacgcaag gattttgtaa gatcttcgct acctctggca
    16681 catggtttgg agacctgggt gggtctacag gagtgagcgg cgcggatcga ctgtgcaacg
    16741 cggacgcaaa caggcctgcg ggtgggggtt tctatgctgc ccttatcagt gacggcataa
    16801 cgcgcattgc cactgtcact tcgaacattg gcgatgggca ggttggctgg gtgatgtatc
    16861 cgaacatgga ataccgcctg gccaatggat ctacagtctg gaccacaaat tcagctggtc
    16921 tgtttgtgtt cgggactgtc acgaaccaga ttgctgctgc aggcacctac tatactggaa
    16981 tggatgcatt ctgggtaagc agcgcgacct gcctctcatg gacagacggg accggaggaa
    17041 atagtggcag gtacggtacc ggcggtgcgt ccggaaacac tgtaatctcc ttgtctcagt
    17101 ccgcctgcaa ttcagccatg cgcggcctgt attgcgtcca gcgttgacgt taggcggcat
    17161 tcaaatactt tgaacgcgtg atccctgcgc agtcaaaact tgttgaagta agcggatctg
    17221 gcgacggcag gacagtagtt gccattggct ttcggatcga tttcaagcgg ctgctgattg
    17281 tttccgctgc cagtaagcgg aaacgcataa atctttccat tgggagcgag aattccaccg
    17341 tcgaacgccg tcaccgcggc tccccacccg gatccaaacg tggtttgcgt atgcgtgacc
    17401 gggtcaaatt caagcactac agcagagctt cttggagtga ggtagatgt
//
LOCUS       DAORFF010000006        17259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1341_length_17259_cov_3.772960, whole genome shotgun sequence.
ACCESSION   DAORFF010000006 DAORFF010000000
VERSION     DAORFF010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 17259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17259
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1341_length_17259_cov_3.772960"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PKK76_00530"
     CDS             complement(<1..654)
                     /locus_tag="PKK76_00530"
                     /inference="COORDINATES: protein motif:HMM:NF022447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNL66974.1"
                     /translation="MNLSRLLSLIAFPILAAIASQCGGETGAIDQDRMQYFKSTYDES
                     RAEFRSLCKGLKGAVGSSLAVASTKESDLTIDTCSVGARVPQKLIIITSGLHGIEGFT
                     GSAVQRFWMKELLSSVDHERTGYLFIHAINPFGFRLERRVTENNVDLNRNFDVTPALF
                     SSHNDGYVKINELLNPDGKLNIGSMGNRFFPVRAVYNIIKHSMRALRQAALQGQYEFP
                     "
     gene            complement(685..1890)
                     /locus_tag="PKK76_00535"
     CDS             complement(685..1890)
                     /locus_tag="PKK76_00535"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HNL66975.1"
                     /translation="MKLPLPAHEDLIVRRQADQLGIYVHFPYCIHKCSYCDFYSAGMD
                     RMPGGGKTVPVSDLESFAAQAQLEYQSRRDQFKKFGRVNSIFFGGGTASLLPAEYVDR
                     LLELFRSDFDTEGAEITVEGNPENFSPTYIESLARIGVNRIHVGVQSLHEHTLRDLDR
                     YYDRDRYSALIETLAHSRIGNRGVDLMYGVPNQTEGQFMADLRLLLSTGLTHVSLYSL
                     TAEPGTPYEAAVRKKKAPSPDEDLQAKLFQELPEFLSHEGFIHYEVSNFARPGFLCRH
                     NLRYWLYEPTLAIGPGAHGFDGSLRYANPRNVIAWQNSPATSPMTMHDPSLEIPLCIL
                     RILVPIAREDLESLFAPLDRGEQGMMLLQKWANQGLADFTGHFQWKSGAVLFLDDRIM
                     EMQEVLSPA"
     gene            complement(1887..3605)
                     /locus_tag="PKK76_00540"
     CDS             complement(1887..3605)
                     /locus_tag="PKK76_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNL66976.1"
                     /translation="MRHLIFLIAAAGVSCARLFSNPIDDLAKTAPAELVKQMTLEEKV
                     GQLIHIGMAGKAMNPALKADIEKYHVGGVILFEINLGKGPEIKALTDSIQDVESKSPN
                     AIPIFVSIDQEGGRVVRVKDGVEQFPGAAAIGQTGDEALAHDAGFITAQDLSALGIPF
                     VLAPVVDVNNNPANPVINTRSYGGDPNLVTRMALAYASGVMDASCIPALKHFPGHGNT
                     GTDSHTSLPTVKQDLAQMDAVELVPYKEGIKRGFPVIMSAHIVFPALDPAEPATLSPK
                     ILKDFLRTKLGFQGLITTDALEMKAVADRYPPPVLARKAFTAGVDVLLLTSSGERTRL
                     MFESLVDGFKKGELPMQDLDRAVERQIELKIRAGLFHKYKMNRPAWSTNVQEAFQVRE
                     EAQKKLRSEIAARYKDGLNRTISRASISSLRKAYNGVGQRADAKLFLRSPASVQQAQE
                     LGLEKQVTVSGFPVGSAAAVIAGFCRPGAQCPAAVIVELADFDLWRWNQLVAEMNDAR
                     KSNRTVPVLVGLYTGNPYLNVRVPSDGAVIGSFSPTEESLRALVYRAFEGGPVREARL
                     VLAEEK"
     gene            complement(3602..4246)
                     /locus_tag="PKK76_00545"
     CDS             complement(3602..4246)
                     /locus_tag="PKK76_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNL66977.1"
                     /translation="MSAIFIDHYAALEIPRTAAGEDVRRAFRRLARVHHPDRGGDALR
                     FHEIYTAYRLLSNEDDRARFDRQLVLRHVRSIMGQRKFIHPSRIVVPTTMAALAKRGL
                     LRKQFRGRDRRMHLRVDYDLELPLGDDELKLPLLVPVPVTLRNICPECRGGDTHCPVC
                     NGRGSYKSGGMFRLNIDGGLVTGQIIEIRLQGMRPAPLQYYKKSTIRIKVTRAA"
     gene            4364..4669
                     /locus_tag="PKK76_00550"
     CDS             4364..4669
                     /locus_tag="PKK76_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66978.1"
                     /translation="MRLAILLVLIFTCGLLAQKDIRSALSADQAAARIEQLLASKPKI
                     VKKYEKKDDTRFLTLTQTTAASFAGSRLPPGQFTLLYMFTRDAEKTIARFEPGKNGV"
     gene            4681..5430
                     /locus_tag="PKK76_00555"
     CDS             4681..5430
                     /locus_tag="PKK76_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66979.1"
                     /translation="MTHPDLFTDPNTNWSLVEQVACKDARDRRHFVHGFALMRGMGRL
                     RPGLERVDEAVSNSDLRPWKNDRQKLEFMFKLAGEIAGNAPVSDACMERIEASIHGKT
                     AQRSADFMEGDPCTDALRSCGNCHLPAKNNCNFTVTYWNEDRQKEANGTVVKEDLRTI
                     TLGGETQQRTIAREDVIQTRWENGADPVEVWWNFKSISSRGSIAKKMLALSAEKQTGC
                     ATCHIGHGDFRMTPAGEEFAKTGVLRSPAQK"
     gene            complement(5632..6423)
                     /locus_tag="PKK76_00560"
     CDS             complement(5632..6423)
                     /locus_tag="PKK76_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66980.1"
                     /translation="MKIYRVFFIILTLAPLSLFAIDGLTRVPEPTPEEQAAYKDVINH
                     IADIQKDIAAYYTDLSEKGRILRTRDASGKEVGGPYSEFEITSEVQTYYGQRDRYVYT
                     ERIHIDWNLQPGTQAGSVVASVKSVVLTQRRGRVGHGYVLKRRIAADVLPKVESESDA
                     LPVNLIVNELLDSGKGLLVNFRFPEAKDVRDKTGENAEVIEVDGQKKKIDLIYVRSHA
                     EKMRMLREYRDALKLTWRRVDWEVRSEKARNARELERILRRQTDS"
     gene            6556..6891
                     /locus_tag="PKK76_00565"
     CDS             6556..6891
                     /locus_tag="PKK76_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL66981.1"
                     /translation="MQIKLKSNNQVTLFELEGSLDIYTSLDLKASLEENVKAKGTTVI
                     LDMEKLTYIDSSGIGIVIKALNYVQGLSGKLIVANLKPPIEKVFRVSGLISYFEILNG
                     EELKKRYPM"
     gene            complement(6892..7482)
                     /locus_tag="PKK76_00570"
     CDS             complement(6892..7482)
                     /locus_tag="PKK76_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66982.1"
                     /translation="MKSLLFFIMILGSPLLAGSPREINFGSEARYGRFMSFIELESWA
                     TGRNLTVLRDCPFRDLDDGEAEIIPAEGMDFSLSSPRDGRVYLYLDMVVFRPAASYNP
                     LTDAIHCQTGGEGVRMNNDRTQELKQVRWLSVEVNGRTLKTVYVGGDVYLRSPIVIPI
                     EREVARGGTLNVRLTVSPGDGFFAIWDTFVSVSPDG"
     gene            7551..8471
                     /locus_tag="PKK76_00575"
     CDS             7551..8471
                     /locus_tag="PKK76_00575"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNL66983.1"
                     /translation="MHIIHDLSTFEPPWTSCALTPGVFDGLHRGHRALIQRLLKKKVD
                     ARILVTYHPHPDLVLGKRKDPEGYELFTYEEKLSLFQSYPLEAVVFLPFTVELARMTA
                     LRYLKEILLGKLRAKNIVIGYDQCFGRGRKGDYKFLKQMSRRYDFEVDRVKAVRGFGP
                     VISSSQIRTYLKQGLIEKAANLLGHDYFLTGTVVRGFQRGRLIGFPTANLEVPRSKVI
                     PAEGVYTAKAEWGGRYYRAMVNIGTNPTFEGKSVSVEAHLLDFDGDLYGQQLRIHFRK
                     HIRNEIKFSSVDDLKSRLEKDRIIARKLKV"
     gene            8484..9068
                     /locus_tag="PKK76_00580"
     CDS             8484..9068
                     /locus_tag="PKK76_00580"
                     /inference="COORDINATES: protein motif:HMM:NF025244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HNL66984.1"
                     /translation="MHNLQEVFERINLINSEDERRLVDPETGTSLPQEVLYSKRMTHW
                     LNVLAPNASDELRVAVFAQHIARFKHPRKTYPEGRTGYLKWKAALLQIHAETTAAILE
                     EMKFPNEFIARVSRLLKKQGLGRDPETQILEDCACLVFIEMFYMDFAPTQGREKMIDI
                     VQKTLRRMSESAKEHAMKLPAAVHVAKLLESSSA"
     gene            9065..9667
                     /locus_tag="PKK76_00585"
     CDS             9065..9667
                     /locus_tag="PKK76_00585"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNL66985.1"
                     /translation="MNSRESPGGSSAHASHVSGVVLCGGHSSRMGRDKGLIERNGIPW
                     ARIIYDSLSLILKNVLVSIREEQRATYEKVFSSEVLIGDSVDTVQGPMRGLISSFLKC
                     DTDILLVGCDMPDLRQSLSHLLQLREENPDYDCLAYRTARVEPLCAIYGKNILKQLRD
                     HHPETFSLERLLERSRTLFIQADENQTAELRNYNEPGPAL"
     gene            complement(9640..10107)
                     /locus_tag="PKK76_00590"
     CDS             complement(9640..10107)
                     /locus_tag="PKK76_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNL66986.1"
                     /translation="MMTGSQATQSIHPRLLTDEPIDLGLFTKQSDLRAGAQVIFAGRV
                     RNHHGGREVNHLEYSAHQKLAPIMISQILREESDKLRLYEAMAVHRIGRVGLGEIAVV
                     VVTSAAHRAEAYEANQRIIHRIKHEVPIWKKEYFADGSWEWGTCDHSAGPGSL"
     gene            complement(10107..10343)
                     /locus_tag="PKK76_00595"
     CDS             complement(10107..10343)
                     /locus_tag="PKK76_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66987.1"
                     /translation="MEIRIQFFASLRDTFGEWLDLSVKEGASAADVLTALADLNPQAA
                     SLLGVSCCATDDRVLGKGEQVPATICVFPPMSGG"
     gene            complement(10346..11332)
                     /locus_tag="PKK76_00600"
     CDS             complement(10346..11332)
                     /locus_tag="PKK76_00600"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNL66988.1"
                     /translation="MTKQSLAESLIVSGAGPLIADAMGRSFATLRLSVTDRCNFACVY
                     CAGDSPFKADSRPFRSAEEIFSSAMPLIEIAGIKKIRITGGEPTLHPGLVDLVALFGK
                     AGLNSIHLTTNGWNLRELVLPLRDAGLTSLNVSLDAAGEEGFRRMARRGGFAGVLHGI
                     DVARALLPVKINCTLVRGQNENEILPVFKLARSLGVTVRYLELMAMGPLRKDARSLLV
                     KEQTILDAIATEESFTPVPTEKSSTAHYFRLTDGYMFGVIANESMPFCSDCNRLRMDA
                     RGNIYGCISDARGININGLQKSELENALRTALSRKQPVRFVGSEKSMQELGG"
     gene            complement(11335..12516)
                     /locus_tag="PKK76_00605"
     CDS             complement(11335..12516)
                     /locus_tag="PKK76_00605"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNL66989.1"
                     /translation="MMIDSASALQLLLDRTPTTVRTESVLLEHSARRVLAQDILADRH
                     YPPFHRSAMDGFAVRFDDLIASRSLSIFATILAGDDPPTSVPPGSAVRIMTGAAVPED
                     LDTVVKVEESQVTGDHVVLSPAKKIWQNIAKRGEDAEPGDQIVLAGRIIDASVQAAAA
                     SCGQDSMRVLAPRIHLITTGNEIVEPGAVPRAVEIRNSNRYTIASLFSYYALHPATVS
                     HVADDPAELKKRLESSLDSELLVLSGGVSAGEADYLPGVMEKCGVEKLFHGVSIRPGK
                     PVWAGFFDSCMVIALPGNPVSTHVTARLFVEPVLRKILGLRREQYPFKRLAKSIAKNH
                     KLQEYLRVTLREDDTLESVPHNGSGDAFASVRATGLAVMPPQSSSIPVGSPVEYIPFL
                     W"
     gene            complement(12506..13417)
                     /gene="moaCB"
                     /locus_tag="PKK76_00610"
     CDS             complement(12506..13417)
                     /gene="moaCB"
                     /locus_tag="PKK76_00610"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HNL66990.1"
                     /translation="MKDIAARINTLRIATASARLKYSPDVEEIVARGITPKGNIYEIS
                     KTAALIGAKQTPMLIPFCHPLSIDGMSVTFEKEPGSIIVRVEAKSIGRTGLEMEVLTA
                     ASVAALNLYDLLKPYDAEMSIEKIQLDKKTGGKSEKWNRIKEGTTAAVLTVSDTVAAG
                     KKPDGSGTAIKDRLTSLGVTVADYRVVADEPQQIQQWVEKWIAEKIPFIFTTGGTGLG
                     PRDVTVETLKKLIQRPVPGIAEAMRVHGGQRTPVAMLSRSVAGTADQSIIVAFPGSTR
                     GVTESMDAIFPAVFHGAGILRGGRHDD"
     gene            complement(13465..13857)
                     /gene="nirD"
                     /locus_tag="PKK76_00615"
     CDS             complement(13465..13857)
                     /gene="nirD"
                     /locus_tag="PKK76_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HNL66991.1"
                     /translation="MNNANLNWMRAGAEADFPRDGGVVVKLGNRQIAVYRFENETDTW
                     YACDNVCPHQNEAVLGRGIIGDKKGEPVVACPMHKKVFSLKSGNCISDAGYSVNVYPV
                     QLKDGSVFVMVHETASNAESLPAEQKVR"
     gene            complement(13861..16335)
                     /gene="nirB"
                     /locus_tag="PKK76_00620"
     CDS             complement(13861..16335)
                     /gene="nirB"
                     /locus_tag="PKK76_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HNL66992.1"
                     /translation="MKRKVIVVGHGMVAHRFIEKLSSATDVEITVFGEEKYPAYDRVH
                     LSSFFDTNSHEGLLLADPAAYAEKGIQLKTGVRVDSIDPAARTIVDSNGTSYPYDILV
                     LATGSTPFVPPLPGRQKTGVFVYRTIDDVMAMRDFSKKATSAAVIGGGLLGLEAAKAS
                     LDMGLKTHVIEFAPRLMPRQIDEDGSSFLCDKIKSLGIELHLGAEVGEIHGDDSVCGV
                     GLKSGEQLSVEMVIISAGIRPRDDLAKSCGLTVGERGGIAVDDSMRTSDPNIFAIGEA
                     ALHRGFVYGLVAPGYEMAAVAADAIVGGERQFTGYDLSTSLKLVGVDVASFGDPFGNK
                     ETKPIVFADRYSGVYKRLNLSADGRRLLGGILVGDASDYGMLTQFTSAGTVLPEDASA
                     LLLGGKSAKMPELAGTAEICKCNNVSKDTIVAAVTAGCTELGALKKQTCAGTGCGGCS
                     PMVSEILNKTLKDLGQTVDMRLCEHFPHTRQELVHIIQIRQLKSFREVLSACGQGVGC
                     EICKPAVASILASSWNDLILRDDVVQDTNDRFLANIQKGGTYSVIPRIPGGEITPERL
                     IVIGQVAKKYNLYCKITGGQRIDLLGARVDQLPQIWEELIDAGFESGHAYGKALRTVK
                     SCVGSTWCRFGVQDSTSFAIRIEERYKGIRAPHKLKSAVSGCIRECAEAQSKDFGLIA
                     TEKGWNLYVCGNGGARPQHAVLLASDISEDLAIKYLDRFIVYYIRTAERLTRTSVWFN
                     SLEGGIDHLRSVIIDDSLGICAELEQQMELLVKNYKCEWNEVVSSPALRARFRPFVNT
                     DETDETIRFEYERGQKKPAEPVGAGV"
     gene            16640..>17259
                     /locus_tag="PKK76_00625"
     CDS             16640..>17259
                     /locus_tag="PKK76_00625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL66993.1"
                     /translation="MSREAITMQSESTLEFKMPEVKTNPRTIEHWDVENESFWKTTGR
                     AIALRNLAFSIFAEFLAFTIWQVWSAVATQLPAVGFKFTLNELFWLAALPGLTGATLR
                     MPYAFMVPIVGGRNWTLISTALLLFPAVGIVMAVQNPETPYWVFLTLALLCGFGGGNF
                     ASSMANINFFFPKRRKGFALGLNAAGGNIGVSVVQFVTPLVIGLSI"
BASE COUNT         4340 a         4662 c         4183 g         4074 t
ORIGIN      
        1 cgggaattca tattgcccct ggagggccgc ctggcgcagg gcccgcatgg aatgcttgat
       61 gatgttatat accgcgcgaa caggaaaaaa ccgattgccc atgcttccaa tgtttaattt
      121 tccgtccgga ttcagaagtt catttatctt tacataaccg tcgttatgcg aagaaaacaa
      181 agcgggagtc acgtcaaagt ttcgattcaa atctacgttg ttttcagtga cgcgcctctc
      241 caggcgaaaa ccaaacggat tgatggcgtg aataaataaa tagcctgtcc gttcgtgatc
      301 cacgctagag aggagttctt tcatccagaa acgctgcacg gcgctcccgg tgaacccttc
      361 aattccatga agcccgcttg tgatgatgat cagcttctga gggactcgcg cgcccacgct
      421 acaggtgtct atagtgaggt cagattcttt tgtagaagca accgcaaggc tcgagccaac
      481 ggctcctttc aaacccttgc acagggagcg aaactctgcc cggctttcgt cgtatgtaga
      541 tttaaagtat tgcattctat cctggtctat cgcgccagtt tcgccaccgc attggctggc
      601 tatagccgcg agaatgggga aagcgatcag ggagagtagc ctggaaagat tcataaccgg
      661 aaggtttcct ggtgcgcgaa gttgtcaagc aggcgaaagg acttcctgca tttccataat
      721 gcgatcatcc aaaaacagaa cagcgccgga tttccactga aagtggcctg tgaaatcggc
      781 gagtccctgg ttggcccatt tttgaagcag catcattccc tgctctccgc gatcgagcgg
      841 cgcgaacaaa gactcgagat cctctcgggc gatcggaacc aggattctca aaatgcagag
      901 tgggatttca agtgacgggt cgtgcattgt cataggagac gtggccggag agttttgcca
      961 ggcaatcacg tttcgcggat tcgcgtaacg caagcttccg tcaaagccgt gtgccccggg
     1021 accaatcgca agtgtgggct cgtaaagcca gtagcggagg ttgtgtctgc aaagaaatcc
     1081 cggccgtgcg aaatttgaaa cttcatagtg aatgaatccc tcatgagaaa gaaactcggg
     1141 caattcctgg aagagttttg cctggagatc ctcatctgga gaaggggcct tctttttgcg
     1201 cacggcggcc tcatacggag tgcccggttc cgcagtcagt gaatacaatg aaacgtgagt
     1261 caggccggtg gaaagcagaa gtctgagatc tgccatgaac tgcccttccg tttggtttgg
     1321 aacgccatac atcaggtcca caccacggtt tccaatgcga gagtgagcca gagtttcgat
     1381 caatgccgaa taacggtctc tatcatagta tcgatcgaga tctcgaagcg tgtgctcatg
     1441 gagagactga actcctacgt gaatgcgatt cactccaatc cttgcaagac tctcaatgta
     1501 tgtgggagaa aagttctccg gattgccttc cactgtgatt tctgcgcctt ctgtgtcaaa
     1561 atcactgcga aacaattcta gaaggcgatc aacatactct gccgggagaa gagaggcagt
     1621 gcctccgcca aagaaaatcg aattcacgcg gccgaacttt ttgaactgat cacgtctgga
     1681 ttggtactcg agctgcgctt gagccgcgaa agattctaga tccgaaacag gcactgtctt
     1741 gccaccaccg ggcattctgt ccatacctgc agaatagaaa tcacaataag agcatttatg
     1801 aatgcagtat ggaaagtgaa cgtagatgcc cagctgatcg gcttgcctgc gaacgatcag
     1861 atcctcatgc gcaggcaaag gcagtttcat ttctcttccg caagcaccag tcttgcttct
     1921 ctcacagggc caccttcaaa cgcgcgatac acaagtgcgc gaagagattc ctccgtagga
     1981 gaaaatgaac caatcaccgc accgtcagaa ggaacacgaa cattcaggta aggatttcct
     2041 gtatacaaac cgaccagaac cggtaccgtg cgattcgact tgcgagcatc gttcatttcc
     2101 gcaacgagct gattccatcg ccacaaatca aagtctgcga gctccactat gactgcggcc
     2161 gggcactgcg ccccgggcct gcaaaaccct gcgatgactg cggccgccga gccaaccggg
     2221 aaaccggaca ctgtcacctg cttttcgaga cccagttcct gcgcctgctg cacagaagcg
     2281 gggctgcgaa gaaacagctt cgcgtccgct ctctgtccga caccgttata cgcttttctg
     2341 agagaagaaa tggaggcgcg tgaaatcgtt ctattgagtc cgtccttgta tctggctgca
     2401 atttcagagc gaagtttctt ctgtgcttcc tctcttacct gaaacgcttc ttgcacgtta
     2461 gtcgaccatg caggccgatt cattttgtat ttgtgaaaaa ggcccgcgcg aatcttcagt
     2521 tcaatttgtc tctccacagc gcgatccaga tcttgcattg gcagctcgcc cttcttaaat
     2581 ccatccacca gagattcgaa catcaatcgc gtgcgctcac cgctcgaagt aaggagcaac
     2641 acatccacgc ccgcagtgaa tgcctttctg gcgagcacag gaggaggata ccgatccgcc
     2701 acagccttca tctcaagagc gtccgtagtg atgagaccct ggaagcccag cttcgtacga
     2761 agaaaatctt ttaagatctt tgggctgaga gtggccggct cagctggatc gagtgccggg
     2821 aaaacgatgt gggcactcat gatcacagga aagccacgct tgattccttc tttatatgga
     2881 accagttcta cggcgtccat ctgcgccaga tcctgtttca cggtcggcaa agaagtatga
     2941 ctgtctgtgc cagtgtttcc gtgacccgga aaatgcttca gcgcaggaat gcacgacgcg
     3001 tccataaccc cggacgcgta ggcaagcgcc atgcgcgtaa ccaggtttgg atcaccgccg
     3061 tacgatcttg tattgatcac tggattggca ggattgttgt tcacatctac aacgggtgcc
     3121 agaacaaacg gaattccaag tgcgctcaaa tcttgagctg tgatgaagcc tgcgtcgtgc
     3181 gccagcgctt catctccagt ctggccgatt gctgcggctc ctggaaactg ttccactccg
     3241 tctttcacgc ggacaacacg gccgccttcc tggtcaatgc ttacgaagat tggaatagcg
     3301 ttaggcgact ttgattcgac gtcctgaata gaatcagtca atgctttgat ctctggtcct
     3361 ttgcccagat tgatttcaaa cagaatgact ccacccacat ggtatttttc aatgtccgct
     3421 tttagagcag ggttcatggc cttccctgcc attccaatgt gaatgagctg gcctactttt
     3481 tcctccagtg tcatctgctt gacgagttcg gcaggcgccg ttttcgccag atcgtcgatt
     3541 ggattggaga atagtcgcgc acagctcacg ccggctgctg cgatcaagaa gattaagtgt
     3601 ctcatgctgc tctggttacc ttgattcgaa ttgttgattt cttataatac tgcagtgggg
     3661 ccgggcgcat tccttgaagg cgaatctcta tgatctggcc ggtcacaagt ccaccgtcaa
     3721 tgttgagtcg gaacattccg ccgctcttgt aggatccacg cccattgcac acggggcaat
     3781 gagtatctcc gcccctgcat tccggacata tgttcctgag tgtcactgga acgggaacga
     3841 gtaaaggtag tttcagttca tcgtcgccta acggcaattc cagatcatag tcaacgcgaa
     3901 ggtgcatacg ccgatcgcgg ccgcgaaact gttttcgcaa caatcctcgc tttgcgagcg
     3961 cggccatggt ggtaggaact acgattcgcg acggatgaat gaattttctc tgacccatga
     4021 tggatcgaac gtgacgcaag acaagttggc gatcaaagcg cgctcgatcg tcttcatttg
     4081 atagaagccg ataagcagtg tagatttcat gaaaacgcag tgcatccccg ccacgatccg
     4141 gatgatgcac tctggcaagt ctgcgaaacg cacgacgcac atcctctccc gcggcagtcc
     4201 tgggaatctc aagcgcagca tagtgatcaa tgaaaatggc cgacacttgc ttcagacttc
     4261 ttcacaaggg ggacgatgca acagtttttt aagaggcttg cccgccttgc cggagagggc
     4321 tgcccccggc tcaagttttc gctgaaagca tcgaaattga agcatgcgcc ttgccattct
     4381 gctcgttttg attttcactt gcggccttct tgcacagaag gatatacgat ccgctctgag
     4441 cgcagatcag gcagcggcca gaatcgaaca gttgcttgca tccaaaccaa agatcgtcaa
     4501 gaagtatgaa aagaaggacg acactcgatt cttaacactc acacagacga cggcggcgag
     4561 tttcgcgggg agtcggttgc ctccggggca gttcacgctg ttgtatatgt ttacgcgcga
     4621 tgcggagaag acgatcgcgc ggttcgagcc aggcaaaaac ggagtctgag cactctccag
     4681 ttgactcatc cggatttatt cacagacccg aacacaaact ggtccctggt agaacaggta
     4741 gcctgcaaag acgcgcgaga tcgtcgccac ttcgtgcatg gctttgccct gatgcgcggc
     4801 atgggcagat tgcggccggg attggagcgc gtggatgagg cagtttcaaa ctcggatctc
     4861 agaccatgga agaatgatcg ccagaaatta gagttcatgt tcaagttagc cggagaaatt
     4921 gcggggaatg cgccggtctc tgacgcatgc atggaacgaa ttgaagcgtc cattcacgga
     4981 aagacagcgc aacgctcggc agactttatg gaaggggatc cgtgcacaga cgcactgaga
     5041 tcgtgcggaa actgccatct tccggcaaag aacaactgca attttacagt cacctactgg
     5101 aatgaggaca ggcaaaaaga agcaaatgga accgtagtca aagaggatct gcgtactata
     5161 acgttaggcg gcgaaacaca acaacgaacc attgcacgcg aagatgtaat tcagacccgc
     5221 tgggaaaatg gcgcagatcc agtcgaggtc tggtggaact tcaaatctat ttcttcacgc
     5281 ggatctatag caaagaagat gcttgcactt tcagcagaaa aacaaacggg ctgtgcgacc
     5341 tgccacattg ggcatggcga ctttcgcatg actcctgcag gcgaagaatt tgcaaaaacc
     5401 ggcgtgctac gttcccccgc ccagaagtga ggatccgtcc gaaccaaatg cgaaaacggg
     5461 cgcaggtctc ctctatttta tcatacgcta aaagtgagac agtgctctgt tgacttctgt
     5521 gaactggatg gataggtggc gccgggttgc cgggccggag ttcacccacc gccataacca
     5581 cccacatccg ggggtgggcg gagtctgccc ccgcgcggga ggcaggagag atcaggagtc
     5641 tgtttgtcga cgcaggatgc gctcgagttc acgcgcattg cgcgcctttt ctgagcgaac
     5701 ttcccagtcc acacggcgcc aggtgagctt tagagcgtcg cgatattcgc gcagcatccg
     5761 catcttttca gcgtgcgaac gcacataaat cagatcgatc ttctttttct ggccatccac
     5821 ttcgatgact tctgcatttt cgccggtctt atcgcgaaca tccttggctt ctggaaagcg
     5881 gaagttgaca aggaggcctt tgccagaatc gagcagttca tttacaatca ggttcactgg
     5941 caatgcgtcg ctctctgatt ccacttttgg caatacatct gcggcaatcc ggcgtttcaa
     6001 aacatagcca tgaccgaccc taccccggcg ctgcgtcagt acgactgact ttacgctggc
     6061 gactacactt cccgcctggg ttccgggctg caggttccaa tcaatatgaa tgcgctccgt
     6121 gtaaacgtac cggtcgcgct ggccgtagta agtctgcact tctgaagtaa tctcgaactc
     6181 ggaatacggc ccaccgacct ctttaccaga tgcgtcgcgg gtgcgaagaa tccggccttt
     6241 ttcgcttaga tccgtgtaat aagcagctat gtccttctga atgtcggcga tatggttgat
     6301 gacatccttg taagcggcct gttcttcagg cgtgggctcc ggaactctcg tcaagccgtc
     6361 gatggcgaac agagaaagtg gagccagagt cagaatgata aaaaatacgc gataaatctt
     6421 catagccgtc cccggttact cttactttcg gcctgggcac cgcaattctt acgtccgacc
     6481 cggcaattct catcgccgca gatacttttt ttcattgatt accccagacc aacatggaat
     6541 atcgccccta tgccgatgca gatcaagctg aaaagcaaca accaggtaac actgtttgag
     6601 ctcgaaggaa gcctcgacat ctacacttct ctggatttga aagcttctct ggaagaaaac
     6661 gtaaaagcca agggaaccac agtgatcctg gacatggaaa agctcaccta catcgactcc
     6721 agtggaatcg gcattgtgat caaagcgctg aattatgtcc agggattgag cggaaagctc
     6781 attgtagcca acctaaagcc acccatcgaa aaagtgttcc gcgtaagcgg tctgatctct
     6841 tactttgaga tcctcaatgg cgaagagctg aaaaagcgct accctatgtg atcagccatc
     6901 cgggctgaca cttacaaacg tatcccagat cgcaaagaaa ccgtctccgg gggacaccgt
     6961 caggcgaaca ttcaaagtgc ctccgcgtgc cacctctcgc tcaataggga tcacaatggg
     7021 agaacgcaga tatacatcgc cacccacgta cactgttttc aaggttcttc cattcacttc
     7081 cactgagagc cagcgcactt gcttgagttc ctgagtgcga tcgttgttca ttcgcacgcc
     7141 ttcgccgccg gtctggcagt gaatcgcgtc tgtgagagga ttgtaggacg cagcggggcg
     7201 aaacaccacc atgtctagat aaagatacac tctcccgtcg cgcggagacg aaagagagaa
     7261 gtccattcct tctgcgggaa tgatctcagc ttcaccatcg tccagatctc tgaacggaca
     7321 gtcgcgaagc acagtgaggt ttcgcccggt cgcccagctc tccagctcaa tgaaggacat
     7381 gaatctaccg tatcgagcct cgctgccaaa attgatttca cgaggagagc cagccaacaa
     7441 tggagagccc agaatcatga taaagaatag aagagacttc atgctacctc ggaattcagc
     7501 ttggcgaaac tggcccacaa tacacgtttc ggcaggtctt tcaactctct atgcacataa
     7561 tccacgatct gagcactttt gagccgccat ggacatcgtg tgctctgaca cctggagtat
     7621 ttgacggcct ccacaggggt catagagctc tcattcaacg cctcttaaaa aagaaggtag
     7681 atgcgcgcat cctggtcaca taccatccac acccagacct ggtgctcgga aaacgcaaag
     7741 acccggaagg atacgagctg tttacctacg aagagaaatt gtctctattt caaagttatc
     7801 ctctcgaagc ggtcgttttt ttgcctttca cagtagaact cgccaggatg acggcgctgc
     7861 gatatctcaa agagattctc ctgggcaagc tccgggcaaa aaacattgta atcgggtatg
     7921 atcaatgttt tgggcgcgga agaaagggag attataaatt tcttaaacaa atgtccaggc
     7981 gatacgactt cgaagtcgac cgtgtcaaag cggttcgggg ttttggaccg gtcatctcca
     8041 gttcgcaaat tcggacatat ctcaagcaag gcctcattga aaaagccgcc aacctgctag
     8101 ggcatgacta ctttctcaca ggcactgtgg ttcggggttt tcagcgtggg cgtttgattg
     8161 gatttccaac tgcaaacctg gaagtgccta gaagcaaagt gatccccgca gaaggagtct
     8221 acacggcgaa ggctgaatgg ggtggcagat actacagggc catggtgaac attggaacca
     8281 acccaacgtt tgaaggaaaa tccgtctctg tagaagcgca tttgctcgac tttgatggag
     8341 acctctacgg gcagcaactt cgcattcatt tccgcaaaca cattcgaaac gaaatcaaat
     8401 tctcatctgt agacgatctg aaatcacgtc tcgaaaaaga taggatcatc gcgcgaaaac
     8461 taaaagttta atcgggagca ttcatgcaca atctgcaaga ggtattcgag cgaatcaatc
     8521 tgatcaactc cgaggatgaa agacgactcg tagatccaga aacaggcaca tctctgccac
     8581 aggaagtact ctattcaaaa aggatgacgc actggttaaa cgttctcgct ccaaatgcat
     8641 ccgacgaact tcgcgtcgca gtcttcgcgc agcatatcgc cagattcaaa catcctcgca
     8701 aaacatatcc ggaaggacgc acaggctatc tgaagtggaa ggcggctctg ctgcaaattc
     8761 acgcggaaac cacagccgcc attctcgaag aaatgaaatt tccgaatgaa ttcattgcgc
     8821 gagtttccag gctgctgaag aaacagggcc tcgggagaga tccggaaacg caaattctag
     8881 aagattgcgc atgcctcgta ttcattgaaa tgttttacat ggactttgcg cccacgcagg
     8941 gtcgcgaaaa gatgatagac attgttcaaa agactcttcg cagaatgtct gaatccgcga
     9001 aagaacatgc gatgaaactt ccggccgcag ttcatgttgc aaagctactt gaatcttcca
     9061 gcgcatgaac tcgcgagaat cccctggagg ttcgtcagct cacgcttctc acgtttcagg
     9121 cgtggtgctt tgcggcggtc acagttcgcg catgggacgg gacaaaggcc tcatcgaacg
     9181 aaacggaatt ccgtgggcca gaatcatcta tgattctctt tcccttattc taaagaatgt
     9241 actcgtttcc atacgagaag aacaacgggc gacttacgag aaggtgtttt cgtcggaagt
     9301 tctgattgga gattctgtgg acacagtgca gggaccgatg cgtggactca tcagctcctt
     9361 tttgaaatgc gacacagaca tcctgctggt tggctgcgat atgccagatc tgagacagag
     9421 cctgagccat cttcttcaac tacgcgaaga aaatccagac tacgattgcc tggcctaccg
     9481 gactgcgaga gtggagcctc tatgcgcgat ttacggaaaa aacatcctga agcaactacg
     9541 cgatcatcat ccagaaacat tcagcctgga acgcttgctc gaacgatctc gcactctctt
     9601 cattcaagca gacgaaaatc agacggctga actcagaaac tacaatgaac caggtccggc
     9661 actatgatca caggttcccc attcccagct tccgtccgcg aaatattcct ttttccagat
     9721 gggcacttca tgtttgatgc gatgaatgat tctctggttg gcctcatagg cctccgcgcg
     9781 atgtgcagca gaagtaacca caaccactgc gatttctcca agcccaactc tgccgattct
     9841 gtgcacggcc atagcttcgt ataaccgcag tttgtcgctc tcttcgcgaa gaatctggct
     9901 gatcatgatg ggcgcaagct tctggtgcgc gctgtattcc agatgattca cctctcttcc
     9961 gccgtggtga tttcgaactc gacctgcaaa gatcacctgg gcccccgcgc gcaaatcgga
    10021 ctgcttcgta aaaagtccca gatcgatggg ctcatctgtg agcagacgcg gatgaattga
    10081 ttgtgtagcc tgtgaaccgg tcatcatcaa cctccgctca tgggcggaaa gacgcaaatt
    10141 gtggccggaa cttgctcccc tttgcccaga actcgatcgt ccgtcgcgca gcaggaaaca
    10201 ccgagcagag acgcagcctg tggattcaga tctgccagtg cggtcagtac gtctgcggcc
    10261 gatgcccctt ctttcacaga caaatccagc cattcaccaa atgtatccct gagggatgcg
    10321 aaaaattgga tcctgatttc catgcttatc ctcccagctc ctgcatggat ttctcgctcc
    10381 ctacaaatcg gactggctgc tttctcgaga gtgctgtgcg aagagcgttc tctagttccg
    10441 atttctgcag cccgtttata ttaatacccc tcgcgtcact gatacaaccg tatatgttcc
    10501 cacgcgcatc cattcgaaga cggttgcagt cagagcaaaa aggcatggat tcatttgcga
    10561 tcactccgaa catataaccg tccgtaaggc gaaagtaatg agcggtagat gatttctcgg
    10621 taggtacggg ggtaaaagat tcttcggttg cgattgcatc caggatggtt tgctccttca
    10681 cgagcagaga ccttgcatct ttacgcaatg gtcccatagc catgagctcc agatatcgta
    10741 cggttacacc cagagatctt gccaatttaa aaaccggcaa aatctcgttt tcattctgtc
    10801 cgcgcaccag tgtgcagttg atcttcaccg gcaacaaggc acgggccacg tcaatcccgt
    10861 gcaagactcc agcgaatccg ccccttcgtg ccatgcgacg aaacccttct tctcctgcgg
    10921 catccagaga aacattcaaa gaagtcaatc cggcatctcg cagaggcaga accagctccc
    10981 ttaaattcca accgttagtt gtaaggtgaa tcgaattgag tcctgccttc ccgaacagag
    11041 ccaccagatc tacgagtcca gggtgaagcg tgggctctcc tcccgtgatt cgaatcttct
    11101 taatgccagc gatttctata agcggcatgg cgcttgagaa aatttcctcc gcactgcgaa
    11161 aagggcggga gtccgctttg aaaggagagt ctcccgcgca atagacacag gcgaagttgc
    11221 agcgatctgt aacgctcaat cgtaacgtcg caaaggatcg tcccattgca tccgcaatca
    11281 atggtcccgc accagacacg atcaaagatt cagccaatga ttgctttgtc atgatcacca
    11341 cagaaaggga atgtattcaa cggggctgcc aaccggaata ctggaggact gtggaggcat
    11401 aacagccaga ccagtagccc gcacagatgc aaatgcgtct ccgcttccgt tatgcggcac
    11461 tgattctaga gtatcgtcct ctcgcagagt cactcgcaga tattcctgaa gcttgtgatt
    11521 ctttgcaatc gatttcgcaa gtcgtttgaa aggatactgt tctcttctta aacccaggat
    11581 cttgcgcaga actggttcta cgaataaccg ggcagttacg tgagtagaaa ctggattccc
    11641 gggaagcgca attaccatac aggaatcaaa aaagcccgcc catactggtt ttcccggacg
    11701 aatggacact ccgtgaaaca atttctccac accgcatttc tccatcacac caggcaaata
    11761 gtctgcttct cccgcagaaa ctccaccgga caatacaagt aactcagaat caagcgaact
    11821 ctcgagccgt tttttaagct ctgcaggatc atctgccacg tgtgagacag tggcaggatg
    11881 caaggcgtag tacgagaaca aagacgcaat ggtgtaacga ttgctgtttc ggatttccac
    11941 agcgcgagga actgcacccg gttccacaat ttcgtttcca gttgtgatca aatgaattcg
    12001 gggagcaagc actctcatac tatcctgtcc acaggaagca gccgcggcct gaacggatgc
    12061 gtctatgatt cgtccagcca gaacgatctg atcccccggc tccgcgtctt ctcccctctt
    12121 cgctatattc tgccagattt tttttgcggg agagagaacc acatgatccc ccgtcacctg
    12181 gctttcttct accttgacta cagtgtccag atcctcagga accgcagcac cagtcatgat
    12241 tcgaacggca ctaccaggtg gcacagatgt cggtggatca tcgcccgcca gaatagttgc
    12301 aaaaatagac aaactgcgtg aagcaatgag atcatcaaac ctgactgcga acccgtccat
    12361 ggcggatcta tgaaagggag gataatgacg atccgccaga atgtcctggg ccagaacgcg
    12421 gcgggcagag tgttcgagca aaacagactc agttctcaca gttgtaggag ttcgatcgag
    12481 taacaactgc aaggcggaag cagaatcaat catcatgcct gccgccgcgc agaattccag
    12541 caccgtgaaa cacagccgga aaaattgcat ccatgctttc tgtcacccca cgggtgcttc
    12601 ctggaaacgc tacaatgata gactgatctg cggtgcccgc gacggaacga gaaagcatag
    12661 caacaggagt cctctgccca ccatgaactc gcattgcctc tgcgattccc ggaacaggtc
    12721 tttgtatgag tttcttaagt gtttctaccg tgacgtcgcg cggaccaagt ccggttccgc
    12781 ccgtggtaaa aatgaaggga atcttttctg caatccactt ctccacccac tgctggatct
    12841 gttgcggttc atccgcgacc actctgtagt ccgccactgt tactccgaga gacgtcaggc
    12901 gatctttgat ggctgtcccg gacccgtccg gtttcttgcc tgcagctact gtatcagaaa
    12961 cagttagcac tgctgccgtt gtgccttcct tgatgcgatt ccatttttcc gatttgccgc
    13021 ctgtcttctt gtccaactga attttctcga tgctcatttc tgcatcgtaa ggcttcagta
    13081 gatcatacaa attcaaagca gccacggagg ccgcggtgag tacttccatc tcgagtccgg
    13141 tgcgaccaat ggatttcgcc tccactctca ctatgataga accgggctct ttttcaaaag
    13201 tcacagacat tccatctata gacagaggat gacagaacgg aatcaacatg ggcgtctgct
    13261 tggctccaat caatgccgcc gtcttcgaaa tctcgtaaat gtttcctttg ggagtaattc
    13321 ccctggcaac aatttcctct acgtccggag aatacttcag ccgcgccgat gctgttgcaa
    13381 ttctgagtgt attgattcga gccgcgatgt ccttcacaac gcccagcgag acaaatcacc
    13441 ggccgctgtc aaacgaacgc gcaactatcg cactttctgc tcagcgggca aagattccgc
    13501 attcgatgca gtttcatgaa ccatgacaaa aacagagccg tccttaagct gcacaggata
    13561 cacattcaca gaatagccgg catcgctgat gcaattccct gacttcaggg aaaacacttt
    13621 cttgtgcatg ggacaggcaa caacgggctc tcctttcttg tctccaatga ttccacgacc
    13681 gagtactgct tcgttctggt gcggacaaac attgtcgcat gcataccatg tgtctgtttc
    13741 attctcaaaa cgatacactg caatctgacg atttcccagc ttgactacaa ctccgccgtc
    13801 gcgaggaaaa tccgcttccg cgccagcgcg catccaattc aaatttgcgt tgttcatatt
    13861 ttatacccct gccccgacgg gctcagcggg tttcttttgt ccgcgttcat attcaaagcg
    13921 aatcgtttcg tctgtctcat ccgtgtttac aaaaggccgg aatcgcgcgc gaagcgccgg
    13981 gctcgacacg acttcgttcc attcgcattt gtagttcttc accagaagct ccatttgctg
    14041 ttcgagttcc gcgcaaatcc ccagagaatc atcaatgatc acgctgcgaa ggtgatcaat
    14101 tccaccctcc agagaattaa accagacact ggtcctcgtg agtctctccg cagttcgtat
    14161 gtagtacaca atgaatcgat ccagatattt gatagccaga tcttcgctaa tgtcagacgc
    14221 cagaagaact gcgtgttgcg gacgcgctcc accatttccg caaacataca ggttccaacc
    14281 cttctctgta gcgatcaaac caaagtcttt actttgcgct tctgcacatt cgcgaatgca
    14341 gccggaaacg gccgacttga gtttgtgcgg cgcgcgaatc cctttgtaac gctcctcgat
    14401 tcgaatcgca aagctcgttg aatcctggac tccaaagcga caccaggtag acccaacgca
    14461 actcttcact gtgcgaagag ccttgccgta cgcgtgcccg ctttcaaagc cagcatcaat
    14521 caattcctcc catatctgag gaagttgatc tacgcgtgcg cccagaaggt caatcctctg
    14581 tccgcctgtg atcttgcagt atagattgta tttctttgca acctgtccaa tgacaatcaa
    14641 gcgttccggt gtgatctctc caccaggaat tcgcggaatc acagaataag tgccgccttt
    14701 ctgaatgttt gccagaaaac gatcgtttgt gtcctgaact acatcatcgc gcaaaatcaa
    14761 atcgttccag gaacttgcga gaatggatgc aaccgctggt ttgcagattt cgcatccaac
    14821 tccctggcca caggcactga gaacttccct aaaagatttc aactgtctga tctgaatgat
    14881 gtgaacgagc tcttgtctgg tgtgtggaaa atgttcacac aacctcatgt ccaccgtctg
    14941 acccagatcc tttagagtct tgtttagaat ttcagaaacc ataggcgaac atccaccgca
    15001 tccagtaccg gcgcatgtct gtttcttaag ggcacccagc tccgtacaac ctgctgtaac
    15061 agcagccacg attgtgtcct tgctcacatt attacatttg cagatttcag ccgtcccggc
    15121 aagctccggc attttcgcgg attttccgcc taatagcagt gcagacgcat cttccggaag
    15181 aactgtcccg gcagaagtga attgagtgag catgccgtag tcggatgcat cacccactag
    15241 aatgccgcct agaagcctgc gaccgtccgc ggacagattc aaacgtttgt atacaccaga
    15301 atagcgatct gcaaaaacaa ttggtttcgt ttctttatta ccgaatggat ctccaaagct
    15361 cgcaacgtct acgcccacca gtttcaggct cgtggataaa tcatagccag tgaattgccg
    15421 ctcaccgccc acaatagcgt ctgctgccac tgccgccatt tcatatccag gagccacgag
    15481 accgtacaca aaacctctgt gcagagccgc ctcgccaatc gcgaagatgt ttggatcaga
    15541 agtgcgcatg gagtcatcca ctgcaatgcc acctcgttct ccgacagtca gaccgcaact
    15601 tttcgcaaga tcgtcccggg gacgaatccc cgcggagata atcaccattt caacagaaag
    15661 ctgctcaccg cttttgagtc caactccgca tacagaatcg tctccgtgaa tttccccgac
    15721 ttccgcaccg agatgcaatt caattccaag cgatttgatt ttgtcgcaga ggaaagaaga
    15781 accatcttca tcaatctgtc tgggcataag cctgggtgca aattcaatga catgcgtttt
    15841 caggcccata tccagcgaag ccttcgcagc ttcgagacca agaaggccgc caccgatgac
    15901 agccgcactt gtggccttct tactgaaatc gcgcatggcc atgacatcat caatcgtgcg
    15961 ataaacaaac actcctgtct tctgtcttcc tggaagaggt ggaacgaaag gagtggaacc
    16021 cgtcgcaaga accaggatat cgtagggata agaagtaccg tttgaatcta cgatcgtcct
    16081 ggccgcggga tcaatggaat ccacgcgaac gccagtcttc agttgaattc ctttttctgc
    16141 ataggcagca gggtcagcca ggaggagccc ctcgtgagag ttcgtatcaa agaaactgga
    16201 aaggtgcact cgatcgtacg ctggatattt ttcctcaccg aatactgtga tctctacgtc
    16261 cgtggcagaa gagagcttct ctataaaccg atgagccacc atcccgtgac cgactactat
    16321 caccttgcgt ttcatggttt atctagagca ataagcatac catggacaga agcaggtcaa
    16381 agcggatagt ttgggacaca atccgcccac ataacaggca attatatgaa tccgtgaaat
    16441 agccgcccga ttttacaccc tattgcctca agtttacaca cactgcgacc cgctcggcaa
    16501 tttgcagaat tcgtccgacg ttctgttgat ctttgcgtct ttcttccatt aaaggaaggt
    16561 gttaggcgaa aatacactgc ggccagccat gccagcagaa caaaacatca cgtggcaccg
    16621 ttcttgctga aggcatccca tgtcgagaga agcaattacc atgcagagcg aatcaacgct
    16681 cgaattcaaa atgcccgaag tgaaaacaaa cccacgcacc attgaacact gggatgtgga
    16741 aaatgaatcc ttttggaaaa ctacgggccg cgccattgct ctgcgcaacc ttgcgttctc
    16801 catcttcgca gagttcctgg ccttcacaat ttggcaggtg tggagtgcgg tagctacaca
    16861 gctccctgca gtgggattca agttcactct gaatgaactg ttctggctcg cggcactgcc
    16921 tggtctaacc ggtgccactt tgcgcatgcc gtatgccttc atggttccga ttgttggcgg
    16981 cagaaactgg actttgattt caaccgcgct gctgctattc ccggcagtcg gcattgtgat
    17041 ggcggtgcag aatccagaga ctccctactg ggtcttcctc actcttgcac ttctgtgcgg
    17101 atttggcgga ggtaacttcg cttcaagcat ggcaaacatc aacttcttct ttcctaaaag
    17161 aagaaaagga tttgccctgg gtctcaacgc agccggcgga aacattggag tgagtgtagt
    17221 gcagtttgtc actcctcttg tgattggtct ttctatctt
//
LOCUS       DAORFF010000007        16969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1377_length_16969_cov_4.022289, whole genome shotgun sequence.
ACCESSION   DAORFF010000007 DAORFF010000000
VERSION     DAORFF010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 16969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16969
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1377_length_16969_cov_4.022289"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PKK76_00630"
     CDS             complement(<1..639)
                     /locus_tag="PKK76_00630"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNL66994.1"
                     /translation="MALFILALAGAIIGIEIFVAISLRSQITSMMEADVKGHVTFAAS
                     YADFRFPGDYRIDPDTGALWKGKENLSTSDSPLVFEIKAYTSSDVTIFRGDTRVATTL
                     RDKSGKSLVGTKASAVVADTVLKQGKIFLGEADVVGEKFQSAYAPLRDRDGKIIGMLF
                     TGLHVDTYSAEMRKTRLAIIVAGLFIIVLFGALLFWIVTRTLRPLFNLIDWTK"
     gene            758..1744
                     /locus_tag="PKK76_00635"
     CDS             758..1744
                     /locus_tag="PKK76_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNL66995.1"
                     /translation="MPLLHVEDLKVHFPTRRGLARAADGVSFDLEEGKTHALVGESGC
                     GKSVTALAIAGLIPRERASISATSLRFRETDLLSLDPRDYRGLRGREIAMIFQEPMTA
                     LNPVMKVGNQILEAIEIHEDSTPTEERKKRVVQLMASTGLAEPDVLYEKYPHQLSGGM
                     RQRIMIAMALALKPAILIADEPTTALDVTVQAQILELMNALQKEMGTAILLITHDLGV
                     VAQTAHTMSVLYAGQVVESGSVRDVFSEPCHPYTQALFQSLPARARNGELVPIPGSVP
                     PATEYDSLHSPCRFYDRCQFKDDRCFQQSTAPGHVAWCRRSAFANNKLNQVH"
     gene            complement(1706..3217)
                     /locus_tag="PKK76_00640"
     CDS             complement(1706..3217)
                     /locus_tag="PKK76_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL66996.1"
                     /translation="MNIPASTRAFKLPAIVILSLAALTFFAAVRSMPVETSVFADGIG
                     KWLQIRGLLTRGDFECAYQESVDPDFQFIPGPWYFYTIQNGKCSYGYQAAYALLAAPF
                     FKIHPLGFLFLHFFLYLAMILGFMLCAHRLLPERSWAPWWAGIIAAWIIPTVVFAMDL
                     SEVILSLAMGAWALALLCKRESFQSIEWKLPSFSEFKYFNSVIFASGIVLGLILSLRT
                     ESLIHGICWAFGLLLVQRKSFVSFASGYAVAILAVMGFHLWFFGNLMGNRGSSHAAVT
                     AGLTASTHIGIARTLLLGGPLGLITSLPAIFLGLFAFSRHARKIHGPQILFMAVISIL
                     PTLGVLITAPSDGGYSWGPRFLSLTFAPYFLLVLLALDHPYAHRWPAMIVLAAIAAYG
                     MQYTWKGRNVYRRAMVQNAGYAAYIESTSSEAIIGADYSLLAILSRSALQGHVYQARS
                     IPEITILADRLKNAGVRRVILLRYATSEVTQLPGWTVKSRKSMDLVEFVVCER"
     gene            complement(3247..3768)
                     /gene="hpt"
                     /locus_tag="PKK76_00645"
     CDS             complement(3247..3768)
                     /gene="hpt"
                     /locus_tag="PKK76_00645"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNL66997.1"
                     /translation="MQSDFEILISGKQIAEKVREIGAQITRDFQNRELVIIGVLRGCF
                     VFMADLARAIDLPVAIDFMEVSSYGDDLESSGNVKIIKDIKDPIHGKHVLLIEDIIDT
                     GHTLNFVVDHLKLKNPASIGIAGLLIKKDKHNFRGKIDYSGFDIADHFVVGYGMDAKG
                     LYRNLDHVAIFRA"
     gene            complement(3803..4948)
                     /gene="thiH"
                     /locus_tag="PKK76_00650"
     CDS             complement(3803..4948)
                     /gene="thiH"
                     /locus_tag="PKK76_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HNL66998.1"
                     /translation="MGLPNPPGFPGIVFTDYYRRMHFASISAEIENASSSDVEHALSK
                     AGTGQISDRNILALLSPAALPYLEEMARMAQSITRQRHGNIIQMYAPLYLSNECRSSC
                     TYCGFSYENNIRRRTLSMEEIEKEGSILYDQGIRHVLLLTGEDYKRTPVQFIADAARR
                     LAKSFSSVSIEVYPLKEDDYSMMREAGVDGLAVYQETYDPECYRRVHLRGMKKNLEFR
                     LECPDRAASAGIRKISIGALLGLSDSATEVFFCAQHARYLMKTYWQTQLSVSLPRLRP
                     AEGLTDVPLVKNRSYVQYLCALRIILPDAGIILSTREGESFRDQMAGICITQMSAGSR
                     TEPGGYSGLEATVQFETEDRRSVHEFCSSLRANGLEPVFTDWSPVMK"
     gene            5009..5758
                     /locus_tag="PKK76_00655"
     CDS             5009..5758
                     /locus_tag="PKK76_00655"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNL66999.1"
                     /translation="MLDSEAMDQIKVAASQLASASSLAVITGAGISAESGIPTFRGEG
                     GLWRKFRAEDLATPEAFRRDPNLVWEWYDWRRQICARALPNAAHEALALLESRMPSFL
                     LITQNVDGLHQRAGSKRILEIHGNIWKARCTACKTKFEISETPLPSLPVLCPDCGSLA
                     RPHIVWFGESYDESLIQEAVSFLRQCDVLLVIGTSGMVSMPGFLTEQAFRAQRIELNP
                     EPSGITPLVQISIRAKAAEAMPLFWERLNAG"
     gene            complement(5769..5972)
                     /locus_tag="PKK76_00660"
     CDS             complement(5769..5972)
                     /locus_tag="PKK76_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNL67000.1"
                     /translation="MKQNMGTVDRIIRIVAALAIAVLAYVNLISGTVAIVLGVLAVVF
                     IATSFVGFCPLYLPFGLSTRPKE"
     gene            6335..7726
                     /locus_tag="PKK76_00665"
     CDS             6335..7726
                     /locus_tag="PKK76_00665"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL67001.1"
                     /translation="MALNHRERIFHLLLVEYGRILQRELMKHPSTLDRPMRDGRSYRA
                     FLETQLAETESITGPVRSGPSILESIYVRFVDKSVLPAQDFDTVTAKNYILAIRSRME
                     AIQAHDEQEEERRIRQSVDIMMRVVNSANTTFTEFNLRENAVRIELLLSLKKELGEEA
                     AIYKEARSMMDRHSLILINYMHELFLDRLKGALEAEQKLREELEKKRQQTEQELRMAQ
                     KIQESLLPARFPEDASLRFAARYLPMSSVGGDFYDAQSISLADQSTGVGVIIADASGH
                     GVPAAFIAAMTKMIWPGSFAGDSDPARALTRMNDSLYERISGNFITVSLGFFQPLDQP
                     PQSLAPSKRGVFHFANGGHQPPVLLRKGESPSLLEAKGRIVGIFPEMSVEARCIDYQA
                     GDRFVFYTDGLVEARKNSGEMLGDESVIAYMDSLRNLPPDEFCDRLIGYVRESTSNCA
                     QDDDVTLFVIDVV"
     gene            complement(7727..9433)
                     /locus_tag="PKK76_00670"
     CDS             complement(7727..9433)
                     /locus_tag="PKK76_00670"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNL67002.1"
                     /translation="MKKQRTLLHDPALYDPYRGLYTRAELEKVRSYLKEELSRSLKHF
                     KKNTSPRPHFISYLFRNFRSEKIAGRLGGITDHTTGSQNTTYCDVRVGGPKFDNVING
                     GLEDNSDRDESIEYINMPAEIEQDSFRFSLWKLTDAKYREAAEQYYERKSKELHYVDP
                     NRGLPSRLKRPPVRHWKYRELPEIDREFWKYRIRLAGNLLKKYANIKNSRFEFNCHHR
                     QSVLVNSEGSMILQQQCIFELRGSLWLLNKKGEGIVQEIHFTEGDIKDLPDEKEFLRI
                     IREKIDRLLILEKAPRMDSYSGPVLLSPVAAGLFFHEVVGHRLEGSRLLSPDEGGTFR
                     SLRGKRIAPDYIDIIDHPSLTAFGNRQMIGHFLYDDEGSPAEKSVLVEKGILKEFLTT
                     STPIPGQKKTNGHARSQKFERPISRMGNLMVVNRKPVSPEEMKERFIEEIRRQKKPYG
                     IHVLDTLGGETGTNSYDFQAFKGEILDATRVFPDGREEPVRGVDFVGTPLSSLDSVMC
                     MGNDPMLDNSYCGAESGVVPVSTVSPSMLMRSLELQGQDRERYTQYVVPLPYSTGKRK
                     KN"
     gene            complement(9405..10985)
                     /locus_tag="PKK76_00675"
     CDS             complement(9405..10985)
                     /locus_tag="PKK76_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL67003.1"
                     /translation="MKKFILLTLVFTVAPVSAQDFYRQGKIAYGQKNYERARQLFEQA
                     MQANPSNGNPCFYLGYMLDNQNRRDLAVQQYRRGVDLQMDADLREKSFWKIVLYYKYV
                     QDWENLAIYSEKFLHYRDIPQVKEFLEEAKERRDPAEGKLRELTASAEKKRAAGDNKG
                     AAADYRSILAIKWDEHSAWNLAALLMQMKEYSEALTMYNRLIDQKPGWEYFYKRGACH
                     YSLRNYDASLQDFNKARTLNKKPDAGFKHFTALGEGLTLIELARYEEAREKLKQSLAL
                     KKSATAAAALSRLEWIHGNRSESERYAALASENEREKNHLTALMLFQSEKKPEERKNG
                     AAAFSKWLASLSDEKYLHRTWTADLFILGRQGCIQANQPLCIPALEKIPEADRFHLGN
                     LVTSAYPTEKRNAADVQRDFDFYYGKGLLDEGKVDAGLSALSRVSDTPVGAYYMAVGF
                     AKKSQEKNAMDYLQRAAEKKPEYWDYAGKNEHFKALAARSSDFAYFLEHKGKPRPVEP
                     AVIPGNEVKNEKAKDTSP"
     gene            11109..13181
                     /locus_tag="PKK76_00680"
     CDS             11109..13181
                     /locus_tag="PKK76_00680"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL67004.1"
                     /translation="MKGILVAICLFCAHSVFGREIALGTDEHLMAGGEAEELRGPFTL
                     DEARVSTSWKDLNGKAPNYGFTREVIWLRLQIHSDEQTERILYLDGWADHLDIYTVHD
                     GVTVYHDAGLKARLTEEGKRFRRIFLPLVFDRGTTQIFFRMESSDTMRFPLEIFTRAH
                     FERFRDTSIALISLYFGGMIVMAIYNLFLFAVTRDKSYSIYVLFVTSSLLYFAAQSGF
                     ILQFFPFVGEVALKRLNLIGSSLVALTMTSFASIFLGLKIGFPRMALIMNVIRVASAA
                     LLVCSFFPAVPYGVCAVLISYVALAGLAMGVISGLVVWKSGYSPARYYLLAFTTLVAG
                     AVVFILGIQGVIGHNILTAHSMQIGSAAEVTLFAIALSDRISLLNRTVDRQMKELESA
                     HALLANSEKKYRTIVEDTTDVIFSLDAEGRIGTINQAARKMLGYWPSMLEGTLFESLI
                     FQRDGISLELGIALFRQSLLTLGEEPFQLRLPLRSRSGEPVDFQVRVEKIGEGSHAFI
                     LRKASAAGEDVLLHFLEIERGSYIIDNYLSTSELLSHRITRNLTRFFDDQAVADIQLV
                     LREMLLNAIEHGNLEVDFDAKTAAQRDGDYLKFLQERQNRSEFRGRRVRVDFGLNQDR
                     VWYRIADEGKGFDHANALRQAQKNLRDTQSTHGRGIGIASRLFDIMQYNAKGNQVFLV
                     KNVHSRRM"
     gene            complement(13197..14216)
                     /locus_tag="PKK76_00685"
     CDS             complement(13197..14216)
                     /locus_tag="PKK76_00685"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNL67005.1"
                     /translation="MALVGCAQYNATPGVYGLALLANSGARCSAPTRASSLSATTTNT
                     TVTAQFSTGACPATSSIVRRSTTGFPATVSSGTLVSSTLTGFSDSGLTAGTVYYYSIF
                     LSDSSGVTSSAANLSAIPGKTVVAPAQVANGSIVIDGSDSDSAWSASPQFSFSYAEAY
                     GESAGADNPSGYVRLAYDSQYFYFFIKSNDKYVHVDGSVCCGQGQWTDDGVEVFFDMG
                     FERATSPDANDFHLIFTPGSPAFNWYGKGPGAWGAWTPTVNSGILINGTLNNETDTDT
                     SWSMEVAIPLSDLGVSSISSGQSIGFTFWLNEDDTVGHAAQHWYPYTTGTSSSNPSTW
                     GVAQF"
     gene            14402..15097
                     /locus_tag="PKK76_00690"
     CDS             14402..15097
                     /locus_tag="PKK76_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNL67006.1"
                     /translation="MIEVKGLSRYYGDFLALDNVSIKIPPGEVVGLLGPNGAGKTTCL
                     RILTGFLFPSSGEVLIDGVDVFSNPLESKKKIGYLPEQPPLYPELLTFDYLQFVGRLR
                     GVEESSLKAEIERVAGLTEISDYLFSPIRTLSLGYRKRVGIAQALMGTPPFLIFDEPI
                     SGLDPRQIVEMRGLLRKIGKDHTVLISSHILTEVRHTCDRVFILHRGKLVREIAGPDL
                     DKIEDLFLQYTST"
     gene            15094..15858
                     /locus_tag="PKK76_00695"
     CDS             15094..15858
                     /locus_tag="PKK76_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNL67007.1"
                     /translation="MNQSNRLTFGLSRIKWIFIKEMKSFFGLNYPPVSLGIIALLCGI
                     VSALLPSTPGTTYEDVSRALFYLYYVFILVDGLLLSIGAFVQERRQGTLELLYTLPVT
                     DLELVLAKFLPGALFISVFSILVTAIYVVGIAEAPYYIALSGALGLILAGLFATSVAL
                     FASSLTGSYLISLLVSAGILLLIEIGGYYGGVLPSPAKEIATHFHGLSQFAPFARGVI
                     SLKACVFFISLTIFFLFLTVRVLESRRWRLQAGGNG"
     gene            15855..>16969
                     /locus_tag="PKK76_00700"
     CDS             15855..>16969
                     /locus_tag="PKK76_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67008.1"
                     /translation="MKNFIDILSDVYSRTGVWIQTVATLTLFTSFSFLDGPTGWVFFA
                     LSAIVIVSDPVVLVLSARSRGARVQSGYLATVVLIVVAVAAYAIRTKLNLTLDTVADA
                     DDAWLTRLRRLLLALFLFSYAGFFVYRLFICIGENATRIQGSADIKRRQMLQKSLISI
                     FAALPLFVLANYLTTVRNPTIDMSPGYYSFGEASRTILKSVPVDVEATVFLPEMQAMK
                     DSSQSKTQPELFRIAEDVRVMADQLPVINGHFKLKYRNADMDLVASQDFGSVNNGTFV
                     FRVMKQGLTTGDNKPYIERRVYVFNESDMERLEREIVRAAIQVSSPEKNVYFTAANGE
                     RWDVPGRVRSPGAIETMKDALRFMNFTLKPLDSQNSWP"
BASE COUNT         4002 a         4209 c         4358 g         4400 t
ORIGIN      
        1 cttggtccaa tcgatcaggt tgaatagcgg acgaagcgtg cgagtaacga tccagaatag
       61 gagcgcacca aagagaacaa tgataaagag acccgctaca atgatcgcaa gccttgtttt
      121 tcgcatctct gcagagtagg tgtccacatg aagcccggtg aagagcatgc caatgatctt
      181 tccgtcgcga tcgcgtaacg gcgcgtaggc ggactgaaat ttttcaccta caacgtccgc
      241 ttcaccgagg aagatcttgc cctgctttaa tactgtgtct gcaaccacag cggacgcttt
      301 tgtgcccacg aggcttttcc cggatttatc tctgagtgtg gtggcaacgc gggtatcgcc
      361 tcggaagatg gtaacgtcag atgaagtata tgccttgatt tcgaaaacga gaggagagtc
      421 gctggtgctg aggttttctt ttcccttcca gagtgctccc gtgtccgggt cgattctgta
      481 atcccccggg aaccggaaat cggcatagga agcagcaaag gtgacatgac ccttcacatc
      541 tgcttccatc atagaagtga tctggcttct cagggaaatg gcaacgaaga tctctatgcc
      601 gatgatcgcg cctgcgagcg caaggatgaa cagcgccata aagcggaatc ggaggccaaa
      661 tcggacgctg cccgtggtca tacattagat tgtcggttgg gtgtggcccg ctcttaattt
      721 gaattgcatg aggaagcagt agaaacgctc tgtgcctgtg cctttgctcc acgtagaaga
      781 cctgaaagtt cattttccca ccagaagagg cctggccaga gcggcggacg gtgtctcttt
      841 tgatctggaa gaaggaaaaa cccacgcact tgtaggggaa tctggctgcg gcaaatctgt
      901 tacagctctt gcgattgcgg gtctcattcc cagggagaga gcaagcatct ccgcaaccag
      961 cctgaggttt agagaaacgg accttctttc cctcgatcca cgcgactatc gtggactgcg
     1021 cggccgtgag atcgccatga tctttcagga gccaatgact gcgctcaatc cagtcatgaa
     1081 agtaggcaac cagatcctgg aggcaattga gattcacgaa gattctactc ccacggagga
     1141 gcgcaagaag cgagtcgttc aactcatggc atccaccggg ctggctgagc cagacgtgct
     1201 ctatgaaaaa tatccgcacc aactgtctgg cggcatgcgg caaagaatca tgatcgccat
     1261 ggctcttgcg ctcaagcctg ccattctcat agcggacgaa ccaaccacag ctttggacgt
     1321 cacagtgcag gctcaaattc ttgagttgat gaatgctctt caaaaagaaa tgggcaccgc
     1381 aattcttttg attacgcacg atcttggagt cgtcgcgcag accgcacata cgatgagcgt
     1441 cttgtacgca ggccaggtgg tggaaagcgg atctgttcga gatgtatttt cagaaccctg
     1501 tcatccgtac actcaggcac tctttcagtc tctgcctgcc cgtgcgcgca atggtgagct
     1561 cgtgccgatt cccgggagtg ttcctcctgc cactgaatat gacagtctcc attctccatg
     1621 tcgattctat gatcgctgtc agttcaaaga tgatagatgc ttccagcaga gcacagcgcc
     1681 cggtcacgtt gcgtggtgtc gccgctcagc gttcgcaaac aacaaactca accaggtcca
     1741 ttgatttgcg cgatttcact gtccatccag gcagttgagt cacttcagat gtcgcataac
     1801 ggagaagaat cactcttctg actcctgcgt tcttgagtct atccgccaga atcgtgattt
     1861 cgggaatgct gcgcgcctgg tatacatgcc cctgcaatgc ggacctcgac aaaatggcta
     1921 gaagggagta gtctgctcca atgattgctt ccgaggaagt agattcaatg taggccgcgt
     1981 atcctgcatt ttgaaccatg gcgcgtctgt acacattcct gcctttccag gtatattgca
     2041 ttccatacgc tgctattgca gccaggacga tcattgcagg ccatctatgc gcgtaaggat
     2101 gatccagagc cagaaggacc aggaggaagt agggcgcaaa agtaagcgac aggaagcgag
     2161 gaccccagga atatcctccg tcgcttggtg cagtgatcag cactccgaga gttggcaaaa
     2221 tggaaatgac tgccataaaa agaatttgag gtccgtggat ctttcgcgca tgcctggaaa
     2281 aggcaaacag tccgagaaat atggcaggca ggcttgtaat tagtccgagc gggcctccga
     2341 gcaataacgt tcgagcgatt ccaatgtgcg tgctggcggt gagccccgca gtcacagccg
     2401 cgtgagaact tcctcgattg cccattagat tcccaaagaa ccagagatga aatcccatca
     2461 ccgcaagaat ggccactgcg tatcctgacg cgaaggaaac aaaactcttt ctctgaacca
     2521 aaagcaagcc gaaggcccag caaattccgt ggatgagact ttccgtgcga agcgaaagaa
     2581 ttagacccag cacaatcccg gacgcgaaga taacggaatt aaaatatttg aattctgaaa
     2641 atgaaggtaa tttccattct atagactgaa aggattcgcg tttgcacagg agagccaggg
     2701 cccacgcacc catggccagg gaaagaatga cttctgaaag atccatggcg aacacaaccg
     2761 tcgggatgat ccacgccgca atgattcccg cccaccatgg agcccaggat ctttccggca
     2821 gtaacctgtg cgcacaaagc ataaagccca ggatcattgc caggtagaga aagaagtgca
     2881 gaaacaagaa tccaagaggg tggatcttga agaacggagc ggctaacaat gcgtatgcgg
     2941 cttgatatcc gtagctacat tttccgtttt gaattgtgta gaaataccac ggtcccggaa
     3001 tgaattgaaa atccggatct acagattcct ggtacgcgca ctcaaaatca ccgcgcgtga
     3061 ggagacctct gatttgaagc catttaccaa tcccatctgc aaaaacactc gtctctacag
     3121 gcatggagcg gactgcggca aagaatgtga gagcagcaag actcaggatt acgattgctg
     3181 gaagcttgaa agcgcgcgtg gatgcgggta tgttcacgca cctttgttgg ttggaattcc
     3241 aatagatcaa gcgcgaaaaa tcgctacatg atcaagattg cgatacagtc ctttcgcatc
     3301 catgccgtag ccgactacga aatggtctgc aatgtcaaag cccgagtagt cgatctttcc
     3361 gcgaaagttg tgtttgtcct ttttaatcag caggcccgca attccaatgg atgccggatt
     3421 cttcagcttg agatgatcca ccacaaagtt aagggtgtgg ccggtatcaa tgatgtcctc
     3481 aatcaagagt acgtgtttac cgtggattgg atccttgata tcttttatga tttttacgtt
     3541 tcccgatgat tcgagatcgt ctccgtagga agatacttcc atgaagtcaa tggccaccgg
     3601 cagatctatg gcccgcgcaa gatctgccat aaatacgaag cagccgcgca atactcctat
     3661 gatcacaagc tcgcggttct ggaagtcgcg cgtgatttgt gccccaattt cccggacctt
     3721 ctccgcaatc tgtttgccgc tgatcaggat ttcaaaatcc gactgcataa accagtttga
     3781 tcagccacac aatgggtgga aactatttca tcaccgggct ccagtcagtg aagaccggtt
     3841 caagtccatt ggcacgcagt gaactgcaga attcatgtac ggatctgcga tcttccgttt
     3901 caaattgcac agttgcctcg agcccggaat atccgcctgg ttccgtgcga gaacctgcgc
     3961 tcatttgagt gatgcagatt cctgccatct ggtcgcggaa ggattcacct tcgcgcgtgg
     4021 acagaatgat tccagcatcg ggcaaaatga tgcgaagcgc gcaaaggtat tggacatagg
     4081 atcgattttt cacaagaggg acgtctgtaa gtccctcggc tggcctgagc ctgggcaacg
     4141 atacactcaa ttgagtctgc cagtatgttt tcatcaagta gcgcgcgtgc tgtgcgcaaa
     4201 agaaaacctc agtcgctgaa tcagaaagtc ctagaagcgc gccgatggaa attttcctga
     4261 ttcccgcaga cgccgctcga tccggacatt ccagtctgaa ctccagattc tttttcatcc
     4321 cacgcagatg cacacgcctg tagcattctg gatcgtacgt ttcctgatac actgccaggc
     4381 cgtcgactcc tgcctctcgc atcatcgaat agtcatcttc tttgagagga tagacttcaa
     4441 tgctcaccga agagaaactc ttcgcgagtc tgcgtgctgc gtctgcaatg aattgcacgg
     4501 gtgtgcgttt gtaatcctct cctgtcaaaa gcagcacgtg tctaattccc tgatcgtata
     4561 gaatgctccc ctccttttca atttcttcca tgctgagagt tcttcttctg atgttgttct
     4621 cgtagctgaa gccgcagtaa gtgcagctgg atctgcattc attggaaaga tacaaaggag
     4681 catacatctg aatgatgttt ccatgtctct ggcgtgtgat gctttgtgcc atccgggcca
     4741 tctcctcaag atacggaagg gcggcgggag agaggagcgc caggatgttt cgatctgaaa
     4801 tttggcccgt tcctgctttg gagagtgcat gttccacgtc gctggaagac gcgttttcga
     4861 tctctgcgga gatggacgcg aagtgcattc ttctataata atcggtgaac acaatgccag
     4921 gaaaaccagg cggattcgga agacccatca aataatttcg ccttgctggt gggtggtctg
     4981 ttttcacaaa tcgactggcg atcaaagaat gctggattcc gaagcaatgg atcagatcaa
     5041 agtggctgcc tctcagctgg cgagcgcgtc aagccttgct gtgatcacag gggccgggat
     5101 ttctgcagaa agcggcattc ctacttttcg tggtgaaggt ggactctggc gaaaatttcg
     5161 tgcggaggat ctggcaactc cagaagcgtt caggcgcgat ccgaatttgg tctgggaatg
     5221 gtatgattgg aggcgtcaaa tctgtgcgcg cgctctgccc aatgcggcgc acgaggctct
     5281 ggcgcttctc gaatccagaa tgccttcttt ccttctgatc actcagaatg tggacggcct
     5341 ccatcaaagg gctggtagca aacgcattct ggaaattcac ggaaacatct ggaaagcgcg
     5401 ctgcactgca tgcaaaacca aatttgaaat ctccgaaact ccgcttccct cacttccagt
     5461 cctgtgtccg gattgtggat ctctggccag accgcacatt gtctggttcg gggaatcgta
     5521 tgatgagtct ttgatacaag aggcagtttc ttttttgcgt cagtgcgatg ttctactggt
     5581 gataggcact tccggaatgg tttctatgcc cgggtttctt acagagcagg catttcgcgc
     5641 acaaagaatc gaactgaatc cggagccttc cggaattact ccactggtcc agatttcgat
     5701 tcgtgcgaaa gccgcggagg caatgcctct cttctgggag aggctcaatg ccggctaacg
     5761 tttagtttct attccttagg acgagtagaa agtccgaaag gaaggtagag cgggcagaat
     5821 ccaacaaagc tcgtagcgat gaaaacaact gccagaactc ccagtacaat tgcgacggtc
     5881 cctgaaatca gattcacgta agcaagaact gcgatggcga gagctgctac aatccgaatg
     5941 attcgatcca ctgtccccat gttttgtttc atagtatcct ctatgttcca atgataggcg
     6001 atccaggtcg ggttcgcaca tgatatttgt catgttttaa cagtgattcc cattgacgtc
     6061 cggtcaggta gttcttactt tgaggcatgt cgtttaagcc ggtcattgca gagaaagccc
     6121 ctttcgtatg cgaagagaag cagggtacca cgaagttctg gtgcgcgtgt ggtctttcgc
     6181 aaaaccagcc ctactgcgat ggaagtcaca agcgcgaaaa gaccgggatg tctcctgtga
     6241 aagttgagtt ttcggaagac aagaaagtgg catggtgcag atgcaaacag acaaagacac
     6301 cgccattttg tgacggttcg catcgcagct gctgatggca ttgaatcaca gggaacgcat
     6361 ttttcatctt ctcctggttg agtacggtag aatactccag agggaactga tgaaacaccc
     6421 gtccactctg gatcgcccca tgagggacgg gcggtcctat cgcgcatttc tggaaacgca
     6481 actggcggaa acggaatcca ttacaggccc ggtccggtca ggtccgtcca tactggaaag
     6541 catctacgtt cgttttgtgg ataagagcgt tcttcctgcc caggatttcg acactgtcac
     6601 tgcgaagaat tacatactgg caatccgctc tcgcatggaa gcaattcagg cacatgacga
     6661 acaagaagag gagcgaagga tccgccagtc cgtagatata atgatgcgag tcgtgaattc
     6721 cgccaacacg actttcactg aattcaatct gcgcgaaaac gcagtccgaa tagaactact
     6781 tctttctctt aagaaagaac tgggggaaga ggcggccatt tacaaagaag caagatccat
     6841 gatggatcga cattctctca tcttgatcaa ctatatgcac gagctctttc tcgatcgcct
     6901 gaagggcgcg ctcgaagcgg aacaaaaact gcgtgaggaa ttggagaaga aacggcagca
     6961 aacagaacag gagcttcgca tggcccagaa gatccaggaa tcactgttgc ctgctcgttt
     7021 tccagaagat gccagccttc gcttcgcggc ccgttatttg cccatgtcca gtgttggcgg
     7081 agatttctat gacgctcagt cgatttcgct cgcggatcaa tcgacgggcg tgggtgtgat
     7141 tatcgctgac gcttctggtc acggagtgcc agcggctttc atcgctgcga tgacaaagat
     7201 gatctggccg ggaagtttcg cgggagattc tgatcctgcc cgtgcgctta cgcgaatgaa
     7261 tgacagtctt tacgaacgca tttcaggaaa tttcatcaca gttagccttg gattctttca
     7321 gcctctggat cagccgccgc aaagtcttgc tccaagcaag cgcggagttt ttcattttgc
     7381 aaatggtgga caccagccac cggttttact caggaaaggt gagtctcctt ccttgcttga
     7441 agcgaaaggc cgtatcgtcg gcatttttcc ggagatgtcc gtggaagcgc gatgcatcga
     7501 ctaccaggcc ggggatcgat ttgtttttta cacagacggt ctggtagaag ccaggaagaa
     7561 ttctggagag atgctcggag acgagtccgt gatcgcgtac atggattcct tgcgaaatct
     7621 tcctccggat gagttttgcg acaggttgat cggatacgtg agagaaagca ctagcaactg
     7681 cgcccaggac gacgatgtga cgttattcgt cattgatgta gtctgatcag tttttctttc
     7741 tttttccagt agaatacggc agcggcacaa catactgagt gtagcgctct ctgtcctgtc
     7801 cctgaagctc gaggcttcgc atcaacatgg agggagacac cgttgaaacc gggaccactc
     7861 ctgattctgc gccacaatag ctgttgtcga gcattgggtc gttccccatg cacatgactg
     7921 aatccagaga ggaaagggga gtccccacaa aatccactcc gcggacaggt tcttctctgc
     7981 cgtccgggaa tactctggtt gcatccagga tctctccttt gaaagcctga aagtcgtagc
     8041 tgtttgtgcc cgtttctcca cccagagtgt ccagaacgtg aatgccatac ggtttctttt
     8101 gtctgcggat ttcttctata aaacgttctt tcatttcttc cggagaaact ggcttgcgat
     8161 tgaccaccat tagattgccc atgcgcgaaa tgggacgctc aaacttctgg cttcttgcgt
     8221 gaccgttggt tttcttctgc cctggaatcg gagtggaagt cgttagaaac tccttcagaa
     8281 ttcctttttc cacgagcaca gatttttccg ccggggaacc ctcgtcgtcg tagaggaaat
     8341 gaccaatcat ctgtctgttt ccaaatgcag tgagagaagg atggtcaatg atatctatat
     8401 aatctggcgc gattctcttg ccgcggagac ttctgaaagt tcctccttca tccggagaaa
     8461 ggagacgaga gccttcgagt ctgtgtccca caacttcgtg aaagaacagg ccggcggcca
     8521 caggcgaaag tagaaccgga cctgaatagg aatccatgcg aggggctttt tcaagaatca
     8581 gcaggcgatc gattttctct cgaatgattc taagaaattc tttttcatcc gggaggtctt
     8641 tgatgtctcc ctctgtgaag tggatttctt gaacaatccc ttctcccttc ttattcaaaa
     8701 gccacagtga accgcgcagc tcgaaaatgc attgctgctg cagaatcatg gaaccttctg
     8761 aatttacgag aacactctgt ctgtgatgac aattgaattc aaaacgagag ttcttaatgt
     8821 tcgcatattt tttgagaagg ttgcccgcga gtcgaattct gtacttccag aattctctgt
     8881 ctatttcagg gagttctctg tacttccagt ggcgaacggg cggtcttttc agacgcgatg
     8941 gcaaacctct gtttggatcc acatagtgga gttcttttga tttgcgttcg taatactgct
     9001 ctgccgcttc tctatatttt gcatccgtga gtttccaaag gctgaagcga aacgaatctt
     9061 gctcaatctc cgcgggcatg ttgatgtatt cgatcgattc gtctctgtca gaattatctt
     9121 ctagaccacc attgattacg ttgtcgaact ttgggccgcc cactcgcacg tcgcagtacg
     9181 tggtgttttg tgaaccggtt gtgtgatctg tgatgccgcc caggcgtccc gcgatctttt
     9241 ccgatctaaa attgcggaag agataggaaa tgaagtgagg gcgaggagaa gtattttttt
     9301 tgaaatgctt gagagaacgt gacagctctt ctttgaggta gcttcttact ttctctagct
     9361 ctgcgcgagt gtatagacca cggtagggat catacagggc cgggtcatgg agaagtgtcc
     9421 tttgcttttt cattcttgac ctcattgcca gggatcaccg caggttccac tggccgtggc
     9481 ttccccttgt gctccagaaa ataagcgaag tcagagcttc tggctgccag cgctttgaaa
     9541 tgctcattct tcccagcata gtcccagtac tcaggttttt tctctgccgc cctttgtaaa
     9601 taatccattg catttttttc ttgagacttc ttcgcaaaac cgactgccat gtagtaggcg
     9661 ccgacaggtg tatcgctcac tctggagagt gcggacaacc ctgcgtccac tttcccttca
     9721 tccagcagcc cttttccgta ataaaagtca aaatcccttt gaacgtccgc tgcatttctc
     9781 ttttcggtcg ggtaagcact agtgactaga tttccgagat ggaagcgatc tgcctctgga
     9841 attttttcca gggccgggat acagagtggc tggtttgcct ggatgcagcc ctgacggccc
     9901 agtatgaaaa gatccgcagt ccaggtccgg tggagatatt tttcatcaga cagagaggct
     9961 aaccatttcg agaaagcagc agcaccattt tttctctctt ccggtttctt ttcactctgg
    10021 aagagcatca gcgcagttag gtgattcttt tcccgttcat tttcagacgc caatgccgca
    10081 taacgttcag attcacttcg attgccatga atccattcaa gtctactcag agcggctgct
    10141 gctgtagcgg atttcttgag cgcaagagat tgcttcagct tttcgcgggc ctcttcatag
    10201 cgagccagtt caatgagagt caacccctct cctagagcgg tgaaatgttt gaagcccgca
    10261 tccggtttct tattcaatgt cctggccttg ttgaaatcct gcagtgaggc gtcatagttg
    10321 cgaaggctgt agtggcaggc cccacgtttg tagaaatatt cccagcctgg cttctgatca
    10381 atcaatcgat tgtacattgt gagcgcttca ctgtactctt tcatttgcat gagcagtgct
    10441 gccaggttcc atgcagagtg ttcgtcccat ttgattgcaa ggatggatct gtagtccgcc
    10501 gccgcacctt tgttgtcgcc cgctgctcgc ttcttttctg cgcttgcggt taactcgcgc
    10561 aatttgcctt ccgctggatc gcgtctctct tttgcctctt ccaggaattc ctttacttga
    10621 ggaatgtcgc gatagtgcag aaatttttct gagtagatgg caaggttttc ccagtcctga
    10681 acatacttgt agtagagcac aatcttccag aatgattttt cgcgaagatc tgcatccatc
    10741 tgcagatcta ctccccggcg atattgttgc actgccaggt ctctgcggtt ctgattgtcg
    10801 agcatgtaac ccagatagaa gcaaggattg ccattggatg gattggcctg catggcctgt
    10861 tcgaaaagtt gtctggccct ttcgtagttc ttctggccgt acgcaatctt tccctggcga
    10921 tagaaatcct gggcagaaac tggtgctacg gtgaaaacaa gagtcagaag gatgaacttc
    10981 ttcatattcc tgaggacaga acgcacgatt gactgttgcg cgcaatgatt tctgagctgc
    11041 ctcgcattcg gacaggttgc cggttaaatc gcttgcatgc gtgagccccg gattccccgc
    11101 tgggatcgat gaaaggcatt ctcgtcgcga tctgcctgtt ttgcgcgcac tcggttttcg
    11161 ggcgcgaaat tgccctgggc accgacgagc atttgatggc cggcggggaa gcggaggagc
    11221 tgcgcggtcc cttcactctt gatgaagcac gcgtatctac gtcctggaaa gacctgaacg
    11281 gaaaggcgcc caactacggc tttacccggg aggtaatctg gcttcgtcta cagattcatt
    11341 ccgatgaaca aacggaacgc attctctacc tcgacggatg ggcggaccat ctggatattt
    11401 ataccgttca cgacggcgtg acggtctatc atgatgcagg tctgaaagct cgactcacgg
    11461 aagaagggaa acgctttcgc aggatttttc ttcctcttgt ctttgacagg ggcacaaccc
    11521 aaatcttctt tcgcatggaa agttcggata ccatgcgttt cccgctcgag atttttactc
    11581 gcgcacactt tgaacgcttt cgcgatacat cgattgctct gatcagcctt tattttggcg
    11641 ggatgatcgt catggcgatc tacaatcttt ttctcttcgc agtgacgcgg gataaatcgt
    11701 attccattta cgtattgttt gttacttcct cgctcctcta ctttgcagct caatccggct
    11761 tcattcttca gttctttccc tttgtaggcg aagtggccct gaagagactg aacctgattg
    11821 gatcgagcct ggttgcgcta accatgacca gcttcgcctc tatctttctg gggctgaaga
    11881 ttggttttcc aagaatggct ttgatcatga acgtgatccg tgttgcgtct gccgctctgc
    11941 ttgtgtgctc ctttttcccg gccgtgccgt atggcgtttg cgctgtgctc atttcctatg
    12001 ttgcgctcgc aggcctggcc atgggagtca tatctggact tgtagtctgg aagagcgggt
    12061 atagtcccgc tcgctactac cttcttgcgt tcacaacact tgtggccgga gcagttgttt
    12121 ttattttagg cattcagggc gtgatcgggc acaacatctt gacggctcac agtatgcaga
    12181 ttggttccgc agcggaagtt acgttattcg ccattgctct gtctgatcga attagcctat
    12241 tgaatcgcac agtagataga cagatgaaag aactcgagtc ggcccacgcg cttcttgcca
    12301 actcggagaa gaaataccgt actattgtag aagatacgac agatgttatc ttttctttgg
    12361 acgcagaagg aagaattggc accattaacc aggctgccag gaagatgctc ggctactggc
    12421 cttcaatgct ggaaggaacc ctttttgagt ctttgatctt ccagcgtgac ggcatttctc
    12481 tggaattggg catagccctg ttcaggcagt cgttgctcac tctcggcgaa gagccttttc
    12541 agcttcgtct tcctttacgc tctcggtcgg gcgagcctgt cgattttcag gtgagggttg
    12601 agaagatcgg agagggatcc catgcattca ttctgaggaa agcgagcgcg gccggggagg
    12661 atgtgctttt gcatttcctc gagatcgaac gtggatccta tataattgat aactatttga
    12721 gtacatcgga attgctgagt caccggatta cgcgaaacct gactcgtttc tttgatgatc
    12781 aagccgttgc cgatatccag cttgtgttgc gcgaaatgtt gttgaacgcc attgaacacg
    12841 gcaacctgga agtggacttt gacgcgaaga cggcggcgca gagggacggc gattacttaa
    12901 aatttctaca ggaaagacag aaccgttcgg aattcagggg aaggcgtgtt cgcgtggact
    12961 ttggtctgaa tcaggacagg gtgtggtacc gcatcgcgga cgaagggaag ggtttcgatc
    13021 acgcgaatgc gctcaggcaa gcacagaaaa acttgcgcga cacgcagagt actcacggcc
    13081 ggggaatcgg aatcgcaagt cgactctttg atataatgca atacaacgcg aaagggaatc
    13141 aggtgttcct cgtgaagaac gttcactcca ggcgaatgta gagcgcggga gatggcctaa
    13201 aactgagcca cgccccatgt actcggattt gaagaagatg ttccggtcgt gtacggatac
    13261 cagtgctgtg ctgcgtgacc taccgtgtca tcttcattca accagaaagt gaagccgatg
    13321 ctctggcccg atgaaatgct cgaaacgccc aggtcactga gcgggatcgc cacttccatg
    13381 ctccagcttg tatctgtatc tgtttcattg ttgagcgtac cattgatgag aatgccagaa
    13441 tttacagtgg gcgtccaggc accccacgct cccggtccct tgccgtacca gttgaatgcg
    13501 ggcgagccag gcgtgaagat gagatgaaag tcattcgcat ccggggaagt ggctctttca
    13561 aaacccatgt caaagaacac ttctacaccg tcgtccgtcc attgtccctg accacagcag
    13621 acgcttccgt ctacatgaac atatttatca ttagatttta taaaaaaata aaagtattga
    13681 gaatcgtaag ccagacgaac atatcctgac gggttgtcgg cgcctgcact ttcgccatac
    13741 gcctccgcat acgaaaaaga aaactgcgga gaagcactcc acgctgagtc cgagtccgat
    13801 ccatcaatga cgatgcttcc gttggccact tgcgccggcg ctacgactgt cttgccagga
    13861 atagcggaca ggttcgctgc gctggacgtg actccagatg agtccgaaag aaaaatggaa
    13921 tagtagtaca cagttcctgc agtcagcccg ctgtccgaaa aaccagtcag agtagaggaa
    13981 accagcgttc cggaagacac ggtagccgga aaacccgtcg tcgacctgcg gacaatggaa
    14041 gaagtggcgg ggcaagcacc ggtgctaaat tgcgctgtga ctgtcgtgtt cgtcgttgtg
    14101 gcggaaagag aagacgctct tgtgggtgcg ctgcacctgg ctcccgaatt cgcaagaagt
    14161 gcaagcccgt agacaccggg ggttgcattg tactgcgcgc atccaaccaa tgccatgaat
    14221 gccacgaagc acccgatgca gtagccgctc agcgagagtg gccgcgatgg cagacgttgc
    14281 cccatgcgtg taacgaacgt gcaatgttcc acccggacaa gaataaattc gtcaaaatca
    14341 gcagagagac atcatagaaa tccgttgacc gcaaactggc tattctgcga ccttaacggc
    14401 gatgattgaa gtgaaaggac tcagccgtta ttacggcgac ttccttgctc tggacaatgt
    14461 cagtatcaaa atacctccag gtgaagtagt tggattgctc ggcccaaatg gtgcaggcaa
    14521 aactacgtgc ttgagaatct tgactgggtt tttgtttccg agctcgggag aagtgctcat
    14581 cgacggtgtc gatgtgtttt caaatcctct ggaaagcaaa aagaagattg gctatttgcc
    14641 agaacaaccc ccgctctatc ccgagcttct aacgtttgat tatttgcagt ttgtgggaag
    14701 gttgcgcggc gtagaagagt cttcgctgaa agcagagatc gagcgagttg caggtctgac
    14761 tgaaatttcg gactatctgt tctctcctat tcgcactctg tctctggggt atcgcaaaag
    14821 agtcggcatt gcgcaagctc tgatgggtac tccgccgttc ttgatttttg atgaacctat
    14881 ttccggtctg gatccgcggc aaattgtgga aatgcgcggt ttactccgca agatcggcaa
    14941 agatcacact gttttgattt ccagtcacat tctgactgaa gtcagacaca cctgtgatcg
    15001 tgtattcatc ctgcatcgcg gcaaactcgt gcgcgaaatc gcaggtccgg atctggacaa
    15061 gatcgaagat ctatttttgc agtatacatc aacatgaatc aatcaaatcg cctaacgttt
    15121 ggtctctctc gaatcaagtg gatcttcatc aaagagatga aatccttttt tggtttgaat
    15181 tatccaccgg tatcgctcgg aatcattgca ctcttgtgcg ggatagtgag cgcgcttttg
    15241 ccaagtacac caggcaccac ctacgaagac gtaagtcggg cgctttttta tctctactat
    15301 gtgttcatcc tggtagacgg actcttgctt tcgattgggg catttgttca ggagagacgt
    15361 cagggaacac tcgagctgct ttacactcta cctgtcacgg atcttgagct ggttctggcc
    15421 aagtttcttc caggtgccct ttttatcagt gtgttctcca ttcttgtgac ggccatctat
    15481 gtggtgggaa tcgcagaggc gccatactac attgcactgt cgggtgcact gggcctgatt
    15541 ctcgcgggtc tatttgcgac gagtgtggct ctgtttgcgt cttcgcttac gggcagttac
    15601 ttgatttcgc ttctggtttc cgccgggatt cttcttctca ttgaaattgg cggctattac
    15661 ggaggagttc ttccctcccc tgccaaggaa atcgcgacgc actttcacgg tctcagtcag
    15721 tttgctccgt tcgcaagagg ggtgatttct ctcaaggcat gcgtgttttt cataagcctt
    15781 acaatcttct ttctgttcct gactgttcgc gttcttgaat cgcgaagatg gcgtctgcaa
    15841 gcaggaggca atggatgaaa aattttattg atattctttc agatgtatat tcgcggacgg
    15901 gagtctggat tcagaccgtc gccactttga ctttgtttac atccttttcc tttctggatg
    15961 ggccaaccgg ttgggtgttc ttcgcattgt ctgcgatcgt gattgtttcc gatccggtgg
    16021 ttcttgttct ttctgcacgt tctagagggg cgagggttca gtccggttat ctggcaactg
    16081 ttgtgctgat cgttgtggca gttgcggcgt atgcgattcg cacgaagctc aacctgactc
    16141 tggataccgt tgcagacgcg gatgatgcct ggctcaccag acttcgcaga ttgcttctgg
    16201 ctctgttcct gttctcgtat gcgggctttt tcgtttatcg tttgtttatc tgcattggtg
    16261 aaaatgctac caggattcaa ggaagcgcag atatcaaaag acgccagatg cttcagaaat
    16321 cgttgatttc gatcttcgcc gcactacctc tttttgttct ggcaaactat ctgaccactg
    16381 tgcgaaaccc aaccatagac atgagtcccg ggtactattc cttcggggag gcatcgcgca
    16441 cgattctcaa aagtgttccg gtggatgtag aagccacggt gtttcttcca gaaatgcagg
    16501 caatgaagga ctcgtcgcaa tccaaaacgc agcctgagct ctttcgcatt gcagaagacg
    16561 ttcgagtgat ggcagatcaa cttccagtca tcaacgggca tttcaaattg aagtacagaa
    16621 acgcggacat ggatctagtc gcctctcagg acttcggatc tgtgaacaat ggaacgtttg
    16681 tgtttcgcgt aatgaagcaa gggcttacga caggggacaa caagccatac atcgagcgcc
    16741 gtgtttacgt attcaatgaa agcgatatgg aaaggctgga gcgtgaaatt gtgcgcgctg
    16801 ccattcaggt ttcctcacct gaaaaaaatg tgtacttcac tgcggccaac ggtgagcgct
    16861 gggatgttcc tgggcgagtc cgctctcctg gtgcgattga aaccatgaaa gacgcgctca
    16921 gattcatgaa ctttactctg aagcctcttg attctcaaaa ctcctggcc
//
LOCUS       DAORFF010000008        16943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1380_length_16943_cov_4.047845, whole genome shotgun sequence.
ACCESSION   DAORFF010000008 DAORFF010000000
VERSION     DAORFF010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 16943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16943
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1380_length_16943_cov_4.047845"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PKK76_00705"
     CDS             <1..377
                     /locus_tag="PKK76_00705"
                     /inference="COORDINATES: protein motif:HMM:NF025923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNL67009.1"
                     /translation="QWAAEIARAHPCIVLEKQRLGDREVEIQGLENLPVGRTPVLVDD
                     MISSARTMIVATELIRAQGFSPVCIAVHPLFHGSAFEDLKASGAATIASCNTITHPSN
                     QIDVSGMLASAIQSMLNEMQKS"
     gene            complement(372..1721)
                     /gene="yegQ"
                     /locus_tag="PKK76_00710"
     CDS             complement(372..1721)
                     /gene="yegQ"
                     /locus_tag="PKK76_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011078568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HNL67010.1"
                     /translation="MKKPELLLPAGSLEKMRIAFDFGADAVYAGQPRYSLRARNNEFR
                     MEELAIGISEAHARGKKFYVTSNIIAHNNKIKSFLKDMAEVAAMKPDAVIMADPGLIY
                     LVRENWPELPIHLSVQASTTNYAAVKFWAKQGVERIILSRELSLKEVEEIKMQCPETE
                     IEVFVHGALCIAYSGRCLLSGYFNKRDPNQGVCTNACRWEYTLHPAAIDDSPRHPLGE
                     YEYLLTEATRPGEFMPIMEDEHGTYILNSRDLRAIQHVPDLMKMGVESLKIEGRTKSL
                     YYVARVTRAYRKAIDDMAAGLPFDESLIEDLEHLANRGYTPGFFGGTIPGDAHNDGAA
                     VSRSDKSRFCGMVASYDSNTKRGFIHVRNFMARGDEIEVLVPSGNHRFVISEMYDESG
                     NPLEVAPGNGWKIHVPMDADLTGGFVVRIASEDSRVSGETDAWKSPVQRDLTAVSAS"
     gene            complement(1755..3389)
                     /locus_tag="PKK76_00715"
     CDS             complement(1755..3389)
                     /locus_tag="PKK76_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL67011.1"
                     /translation="MKITYTNDCTVEETDSSLSLLQISLKHEIPHTHVCGGNARCSTC
                     RILVLKGAENLEPRNEAETALALRKGFTDDIRVACQTRAHGDVTVRRLVIDQDDIREI
                     VGTSIHTTGREMNLAILFSDIRSFTPFSENHLPYDVVHILNRYFNAMGEVILRNGGII
                     DKYIGDGLMAIFGLNSSDARTACLNAVRAGSSMLAELEHVNRYLQRTFQVNFNIGVGV
                     HYGEMIVGEMGHPKKVQFTALGDNVNMASRIESATKKAGVPFLISDAVYEHVKDQVTR
                     GKVFSAPLKGKTGRFKLYEIVEVAPGQGGTSEQYQLPLLQKMQIAQDTWAFLLDTSLN
                     DYAFEPGQFADLTLPEMESVPGQSTRTFSIASSPDRSDALMFVSRMRNSPFKNALMKL
                     PEGAPIFVSPAMGEMTIKNTPAPLAFIAGGIGITPFHSMVSWVLKEKPGTEVYLFYSI
                     RSLELAAFLDEFETYAAEFPNFHFIPTLTEADSPGWKYERGRIDKVMIRKYVPRDAYP
                     VFYVAGPSRMVTGMRELLVQMGVFDHDIHSEEFLGY"
     gene            complement(3392..4279)
                     /locus_tag="PKK76_00720"
     CDS             complement(3392..4279)
                     /locus_tag="PKK76_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67012.1"
                     /translation="MMRVCLTISIFVFLLSCRSAATRHELEYGGRAREYLVIPVEQPR
                     SMIFVLHGGGGKAAHMIRLAEGLPEIAKQRHILLVYPQGIDKGWNDGRDTKISTAISE
                     NVDDAGFLDSLALKLAADYKIPEDKIFITGISNGGMMSQRLACESRAFRAIASVAANL
                     QESFAPKCKPSAPISTLFILGTEDPLVPYGGGDISLFKKTRGRVLSADATLQFWRSHN
                     QCNADSVKTDIPDAVKDNTSATAETWSCGNARVRLIRVLGGGHTWPGGLQYLPEVVVG
                     RTSRDFSASEEIVRFFLGD"
     gene            complement(4286..6499)
                     /locus_tag="PKK76_00725"
     CDS             complement(4286..6499)
                     /locus_tag="PKK76_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HNL67013.1"
                     /translation="MSTASRLLRYLEQNAGRKLPRKEVLSAFSSTGAFKAKAKSFRKS
                     RQVRGYQAESEVDVLLAELKLLNLVRTDGNKIIPASPFAAVAKASLSPSGAVFGAVRG
                     ADPASRDLFIPAAKSMNALTGDLVLVKLTDKTRDRFEGEVIEIREKARELYRMKLLSE
                     PVHRGIPGVLLDIHSRITACLATPRLPADLVSRMKQDVVVVVRMPGKTIMHMGLPLPE
                     AEFVRFESDTDMDPDYARVLMKHNLDPVYPEVEGLPEYDQEASEVKPENVFDWNSRID
                     LRDLYTVTIDGPDSKDFDDAISLKVESPRLWKLYVHIADVSHYVRIGTDLDREAARRA
                     TSVYLANRVVPMLPPVLSENFCSLIAERNRLAFTCEMDVRPDTGEILKYQIYKSVIRV
                     DKRLTYDLAETMLDESDSMLGRVWSLAKAQKAARLKAGRIDFDMREPKVIYDAEGRIK
                     EVLYRERLKSSMLIEECMLSANICTAEFQRKKEAHTLFRVHEPMDDMKLEALNHFLKI
                     YGINLELPNSDPENINHALKVVHEFTEKGKSVQKGSTRKLEGVPPDRIFQMLMLRSFM
                     QAKYMGEPLGHYGLGFRDYCHFTSPIRRYPDLVVHRALQNLIQKKKHAYTEAEISELG
                     FHTSEMERKAMDAERDIWKLKMLRSIERSGQKSFRGFITGFRPDRVFLELTDVSVEAI
                     VLHSHLTRDMELILPDPFSAYIKKLSRPAFLGEVWNLELERLDVEEMRLFVKPVW"
     gene            complement(6496..7158)
                     /gene="tsaB"
                     /locus_tag="PKK76_00730"
     CDS             complement(6496..7158)
                     /gene="tsaB"
                     /locus_tag="PKK76_00730"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNL67014.1"
                     /translation="MNNYCLCIDTTHTHIVVGLFSKQNQYQIVRKAPREAFQNLFPAI
                     QEVLSAANVKRPSEIVTLTGPGSFTGIRICVSAARDLAQLWNVPVRGIGTLTCYSLPI
                     RNMEPGPFAVCIDGKQNRFYTKIIPESGLSATQMDLIPVQDLTAEEVAASGLPVYCDD
                     PDTFRARNGGDSRQLKEPEAAWIFAAHSEMPASGSYQELFPYYLRKDPAEARYPGGFN
                     IK"
     gene            complement(7133..7612)
                     /gene="tsaE"
                     /locus_tag="PKK76_00735"
     CDS             complement(7133..7612)
                     /gene="tsaE"
                     /locus_tag="PKK76_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNL67015.1"
                     /translation="MSLRKIDSALQMEEACCAFFAQFLESLPVSVVLLRGEPGAGKTT
                     FCRGFMRAMSIRENINSATFNLHNRYEGSRGVLHHYDLYRLQKVAELEELGFLEDWHR
                     VDHAPTVHAIEWGDRALPHIGPGVPVFLVEISIDEKDDSLRHIQFYRRQGEQLLSLH"
     gene            complement(7624..7884)
                     /locus_tag="PKK76_00740"
     CDS             complement(7624..7884)
                     /locus_tag="PKK76_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67016.1"
                     /translation="MRAARKVLYTIVFSVFFFIFAGVARDIMERFQPELVQIPSVITG
                     IFLLKWIGIFAGVVLVSFWGAGAVVYKNLSGRDLSDRDDWRR"
     gene            8056..9180
                     /locus_tag="PKK76_00745"
     CDS             8056..9180
                     /locus_tag="PKK76_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67017.1"
                     /translation="MKKYLGIGLILAVSSMAPLRAQIVCTGPGCQYLPLSQATLDQMY
                     YDFKTQYTDQLFKDMAAAGVLANLTGAPTGTVNLYGWTIGASAGLGYIPIKEVSIPIT
                     GYGELKDIPRAGGAVNPRIFFGANVGWLLNQAYDPWGDGESSEPFFLSLSRFDLYGSF
                     IQDRRHYVMQDSGQVTVNTRNLGFDVRYHLVEGKPLAAGPLLRFLGVSVGAGYHTAAM
                     DITAIQNQNQKMKITLTGGSNLIWDGANQGKIQTQVKSMPLEIMTGLQILYALNMSFG
                     GGMSINRGSADVTLTRLGPVYLSNDSAALLAAATGQTVASSAYNSYLTMQVTGSGQAP
                     RNMAYAKLGLDFNILWFKIFAEGLWAKRAYGANLGVRWQM"
     gene            complement(9184..9948)
                     /locus_tag="PKK76_00750"
     CDS             complement(9184..9948)
                     /locus_tag="PKK76_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNL67018.1"
                     /translation="MTFVFKRLTLVAILLVVVSACRVAEPRGNSMGQDYLEREFKCTE
                     TPLPVEVDPGFGLIKQAVRQNWGNENRKSARIVLTGDSTIALFAGPRLQAYLPGLDVA
                     NRGIGGDTSGGLLARLDTDVVALHPSTVVIVIGGNDILQGRCMRTVLANTTSIFKTLK
                     ESLPGVRIIMVSIPPVVSWKANSITPYYNRKLEYLTRDEGIEYLDLWPALSDSDKPQL
                     KEEFYFRLPGGKVDQVHFNDKGYETFGRLLQPLLSR"
     gene            complement(9945..11234)
                     /locus_tag="PKK76_00755"
     CDS             complement(9945..11234)
                     /locus_tag="PKK76_00755"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNL67019.1"
                     /translation="MFGPIKKRKDPLTILVSIIIILLFAFLAGTAALVMAAFLAVLVL
                     FYPFFLYWWDKLYGTRDIADQLFYAHTDDGWNIAMHFHMPRYPKANAYPVIMSHGIAV
                     NKYGVDLDEAHSLPYFLKQNGYPVFVLSLRGCGKSYHSSKGRYRDFNFDDIVMYDVPA
                     VIRRVKELTGAPKVNWVGHSMGSMIMYGFLGRELPGSEDIACFTALASPGKIDHAKTT
                     IWGAIANMPWLPRVLDMKFGAQIISPLTGRFLTPIDNIVYDSENISKVTIRRLMKNGI
                     ENIGAGLISQFLDWIKSGVEHTRDGSHSYRDGFKNIKAPALFLAGTRDHIAPAESVRF
                     AYEQCGSKIKEFRLLGKATGFHCDYCHTGIVLGDRARDDVYPLVRDFLDEYGMKKRRR
                     NFIKAIFKRFIRKRRQRQLKGLRIFREPAKPGSIIVS"
     gene            11280..11711
                     /locus_tag="PKK76_00760"
     CDS             11280..11711
                     /locus_tag="PKK76_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67020.1"
                     /translation="MQSESGGYQTFQGPFFSIRLPSHWEIEVIENMPAFFDPEGNGVL
                     QVAALKRQGQDYDIQAELERYLAQNQIEADPERMSRFRLPSGLNGIAVEYIVDNRFWM
                     VNALGAGNQCLLAIYNSDEVPDQGMAMLISEALASIEFMHS"
     gene            11708..12313
                     /gene="purN"
                     /locus_tag="PKK76_00765"
     CDS             11708..12313
                     /gene="purN"
                     /locus_tag="PKK76_00765"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNL67021.1"
                     /translation="MKRIVVLASGRGSNFRAVLSAIEDHRIAGAHILALVSDRSGTGA
                     EQIARDANIPITLVDYSDKSKRASSDENLTKVISNLAPDCVLALGFMRILPEKLVLAF
                     RHKIINVHPSLLPAFPGMNAQKQALEYGVRVTGATVHFVDTGVDTGPIILQSAVQVPA
                     GATLEEITDLIQAEEHRIVVQALDLFLKDQIQVDGRKVLIK"
     gene            12327..13961
                     /gene="purH"
                     /locus_tag="PKK76_00770"
     CDS             12327..13961
                     /gene="purH"
                     /locus_tag="PKK76_00770"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNL67022.1"
                     /translation="MIQIKRALISVSDKTGLNDFARFLAGRGVDIISTGGTLKTLQEA
                     GIKARAVEEVTGFPEIMDGRVKTLHPKIHGGLLSLRDKQSHLDAMKAQQIEGIDLLIV
                     NLYPFAATIARDASFEDSIENIDIGGPAMLRAAAKNYRFCAAVTDPSDYSRLKEEIER
                     AGGIPEKMSLNLARRVFHHTAEYDSLISQYLDQIEAVKFPDSLTIAFRKVQPMRYGEN
                     PHQEAAYYRPLIDIERERRTGGLWHQIQGKELSYNNMLDANAALMSALSLPGHGVVIV
                     KHLNPCGVALASMDPALGIFGATPGQEGSANVQTFQGGMPEAFRLARACDPVSAFGGV
                     IALTDLVDLETANLINENFAEVIIAPGYSPEALDAFSAKKNLRLLEIKRPERFLAPGM
                     ELRLNELGLLYEDMDHTYAPRSTWKVVTNRAPEAAELNALELAWRVVKHVKSNAIVFT
                     NERSTLGIGAGQMSRVDSTEIAASKAKKAGLSLATSCVASDAFFPFRDGVDALARAGA
                     RAVIQPGGSVRDEEVIQACNEHGIAMVFTGMRHFRH"
     gene            14065..14961
                     /locus_tag="PKK76_00775"
     CDS             14065..14961
                     /locus_tag="PKK76_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67023.1"
                     /translation="MNSCIALQKLELFQQGKNSFGVAQSAFLQGDAKLIDQFISQSEA
                     NVDLARYFLGVQKPEDSISIASSLKADALYIIFKRDYAVFLQKQKQKAPADRETNNYF
                     LQKATQYWSKLPVAKICTVIAYIVREKKDVSSAAQFMVLIPSSVISRFPELSGLTEDE
                     ERELFLGLEGDLFTLPLLSPGIYDHMMTLFAEQPEISMVLAGMEELVKRRSVVISTTD
                     NMVEYYNKTNSRLSLPALYSELFGIEFELAAEVLHHLVEHQVITTNERNTLLEFLKSG
                     KLDFIRQMREDILSVSPLPRTD"
     gene            complement(14958..16274)
                     /gene="add"
                     /locus_tag="PKK76_00780"
     CDS             complement(14958..16274)
                     /gene="add"
                     /locus_tag="PKK76_00780"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNL67024.1"
                     /translation="MVMRLDDVLQRIRTLDRDVEELRRLRARLPANRAFTSGMQVAFD
                     RHINELLNEKIQLEELEVEHIPEDLLNDIASVDVATASRITVDREKKEYELTSEDKKI
                     MEFIRAMPKTEIHLHMEACINQQTLQGMLDKNGVPYESEQLKKLYQFTNLQEFIKLFL
                     FIIDSIKMAEDFALIFKNLRQYLESNNIRYAEVFLAPSRMIQNGLDYRDIADSLDSLS
                     RECRNSGGPDVRFLIDVSRTFGPENASKNLQRVLSNPRPSIIGIGLGGAELMGPARDY
                     RDVFAQAQAEGLRTVAHAGEDDGPWSIRDTVELLKAERIGHGTSAVQDPALLELIRKK
                     AIPIEICLTSNIFTGKYVRREKDHPVRRYYEEGIICTVNTDDPEIFHVTLSDEYFKFH
                     KSLNFTVSEIVDLCRQGVYATFQHNPATMWNQFKPQIDDLRKKFAL"
     gene            complement(16277..16822)
                     /locus_tag="PKK76_00785"
     CDS             complement(16277..16822)
                     /locus_tag="PKK76_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNL67025.1"
                     /translation="MEQKRSARRTSLRYKFQLRLVRRAMLPLAVLSAAAMVVIWSATT
                     GRHEYSMDELRWLGIGGVALLFVASLVGVGWSLMLYSSRLAGPLVHLEKTLAAIAAGD
                     LSTRVHLRKDDELQEHAAHLNRTFMSLQDRVKRIHQYCRYARETVDRLAASPDVKDKD
                     QLDQMRQLIGFIEEALQDFKV"
BASE COUNT         4295 a         4434 c         4091 g         4123 t
ORIGIN      
        1 gacaatgggc cgcagaaatc gcccgcgcac atccctgcat cgttctcgaa aaacaaagac
       61 tgggagatag ggaagtagaa atccagggcc tcgaaaatct tccagtggga cgcacgccgg
      121 ttctggtgga tgatatgatc tcaagcgcca gaaccatgat cgtagccacg gagctcattc
      181 gagctcaggg attttctccc gtttgcattg cagtgcaccc gttgtttcat ggaagcgcgt
      241 tcgaagattt gaaagcttcc ggagcggcga cgattgcttc atgcaataca atcacccatc
      301 cgtccaacca gattgacgta agcggaatgc ttgcctccgc gattcaatct atgctgaatg
      361 aaatgcaaaa atcatgaagc gctaacagca gtaaggtcac gttgcactgg acttttccac
      421 gcgtcagttt ctccagagac tctggaatct tcagatgcaa tgcgaactac aaaaccacct
      481 gttagatccg catccatagg aacgtggatc ttccatccat ttccgggcgc tacctcaaga
      541 ggatttccag actcgtcata catttcagaa attacgaacc tgtgattgcc ggacggcact
      601 agaacttcga tttcatctcc acgcgccatg aagtttcgca cgtgaatgaa tcctctcttt
      661 gtgttcgagt cataagacgc taccataccg cagaaacgcg atttgtccga acgcgataca
      721 gccgcaccat cgttgtgagc gtctcctgga attgtaccac caaagaaccc gggtgtgtaa
      781 ccgcggttgg caagatgctc caaatcctcg atgagcgatt catcaaaagg aaggcccgct
      841 gccatatcat caatggcctt gcgatacgcg cgagtcacac gtgccacgta gtacagagat
      901 tttgtgcgac cttcaatctt gagactttcc actcccattt tcatcaaatc cggcacatgc
      961 tgaatggcgc gaagatcacg tgaattcaat atgtatgtcc cgtgttcatc ttccatgatg
     1021 ggcatgaatt cacccggtct cgtggcttct gtaagaagat actcatactc ccccagtgga
     1081 tgacgaggag aatcatcgat cgcggccgga tgaagagtgt attcccatct gcaagcgttt
     1141 gtacaaactc cctggttcgg atcacgctta ttgaaatacc ccgaaagaag acaacgacca
     1201 gaataggcga tgcagagtgc accgtgaacg aacacttcaa tttccgtttc cggacattgc
     1261 attttgattt cttctacttc tttcaacgaa agttcgcgcg acaaaatgat tctttctacg
     1321 ccttgctttg cccagaattt caccgcagcg tagttcgtcg tagaagcctg cacggaaagg
     1381 tgaatcggaa gctccggcca gttttcgcgc accagataaa tcaaaccagg gtctgccatg
     1441 atgactgcat ccggtttcat agcagccact tctgccatat ctttgagaaa gcttttgatc
     1501 ttattgttgt gcgcgatgat gttagatgtt acataaaact tttttccgcg cgcatgagcc
     1561 tcggagatac cgatcgcgag ttcttccatc ctgaactcgt tgttacgcgc acgaagactg
     1621 taacgcggct ggcctgcata cactgcatcg gcaccaaaat caaatgcaat gcgcatcttc
     1681 tcaaggctgc cggctggcaa gagcaactct ggctttttca taaccaggtt tgccggcatc
     1741 cctcgcagaa gcaatcaata gcccagaaac tcttcgctgt gtatatcgtg gtcaaatacg
     1801 cccatctgga ccaggagttc ccgcattcca gtcaccatac gagaagggcc ggccacatag
     1861 aacaccggat aggcgtcccg gggcacatac ttgcgaatca ttactttatc gattctgcct
     1921 ctctcatact tccaccctgg actatctgct tccgtcaagg ttggaatgaa atgaaagttt
     1981 ggaaattcag cagcatacgt ttcaaattcg tctagaaatg ccgccagctc cagggaacga
     2041 atcgaataga acagatacac ctctgttccc ggcttctctt ttagaaccca gctcaccatg
     2101 ctgtgaaacg gagtgattcc aattccgcct gcaataaacg caagaggggc cggagtattt
     2161 ttgattgtca tctcgcccat ggccggactc acaaagatcg gagcaccttc gggcagtttc
     2221 atgagagcat tcttgaaggg actgttgcgc atccgcgata cgaacatcag cgcgtcggat
     2281 cgatcaggcg aagaggcaat ggagaacgtg cgagtcgatt ggcccggcac agattccatc
     2341 tctggcagag tcaaatccgc gaactgtccg ggctcaaacg catagtcgtt cagcgatgta
     2401 tcgagcaaga atgcccacgt gtcctgagca atctgcatct tctgcaacag cggaagctgg
     2461 tactgttcag acgtcccgcc ctgacccgga gctacttcca caatttcgta gagcttgaat
     2521 ctgcctgtct tccctttcaa cggagcgctg aatacttttc cacgcgtcac ctggtccttc
     2581 acgtgctcgt agaccgcatc tgaaatcaaa aacggaacgc cagccttctt ggttgcagat
     2641 tcaatcctgc ttgccatgtt cacattatca ccgagtgccg tgaactgcac tttcttgggg
     2701 tgtcccattt ctcccacgat catctcgccg tagtgaacgc ccacgccaat gttgaaattc
     2761 acctggaacg ttctctgcaa atatcgattc acatgttcta attccgcgag catggatgaa
     2821 ccagcgcgaa cagcattcaa gcaggcggtg cgagcgtcag atgaattcaa tccgaagatt
     2881 gccatcaatc cgtctccaat gtacttatcg atgatcccgc catttcgcaa gatcacttct
     2941 cccatcgcat tgaagtatcg attgagaatg tgaacaacat cgtacggaag atgattctct
     3001 gaaaaaggag tgaagctgcg aatgtcgctg aatagaatcg cgagattcat ttctcgcccc
     3061 gtcgtgtgaa tggacgttcc cacaatttcg cggatgtcat cctgatcaat gaccagacga
     3121 cgaaccgtaa catctccatg cgcgcgagtc tggcaggcca cgcgaatgtc gtccgtgaag
     3181 ccttttctga gtgcgagtgc cgtttcagct tcgttgcgtg gttcgaggtt ttctgctccc
     3241 tttagaacca gtattctaca cgtagaacaa cgcgcatttc caccgcaaac gtgcgtgtgc
     3301 ggtatttcat gtttcaagga gatttgaagc aaactcagag acgagtctgt ctcttccact
     3361 gtgcaatcgt tggtgtatgt aatcttcaaa gtcaatctcc cagaaagaat cgaacgatct
     3421 cttcgctcgc agaaaaatca cgcgacgtgc gccccacaac aacttccggt agatactgca
     3481 aacctcccgg ccatgtgtgt cccccgccaa gcactcgtat caatctgact cgagcattcc
     3541 cgcaagacca ggtttccgct gtcgcgcttg tgttgtcttt gacagcatcc ggaatatccg
     3601 tcttcacaga atccgcattg cactggttat gcgatcgcca gaactgcaga gtggcatccg
     3661 cggacaggac tctacccctg gtcttcttga acaaagagat gtccccgccg ccgtaaggga
     3721 caagaggatc ttccgttccg agtatgaaca gagtagaaat gggtgcggac ggtttacatt
     3781 taggcgcaaa tgattcctgg agattggcgg ccacggatgc gatcgcgcga aacgcccgac
     3841 tttcgcatgc aagccgctgc gacatcattc cgccgttgga aatgccagta ataaaaatct
     3901 tatcttcagg gatcttgtag tccgcggcga gtttgagcgc cagagaatcc agaaaccctg
     3961 catcgtctac gttctccgaa atggccgtgg atatcttagt atcgcgcccg tcgttccatc
     4021 ccttgtcgat tccctgagga tacaccagca gaatatgtct ttgctttgcg atttcaggca
     4081 gaccttccgc cagtcgtatc atatgtgctg ctttgccgcc accgccatga agcacgaaga
     4141 tcatggagcg aggctgctcc acgggtatta ccagatactc gcgagcgcgt ccgccatatt
     4201 ctagctcatg ccgagtggcc gcgctcctgc aggaaagtag aaatacgaaa atgctgattg
     4261 tgagacagac gcgcatcatt cccccttacc agactggttt tacaaacagc ctcatctctt
     4321 ctacatccag acgttctagc tccagattcc acacttcgcc cagaaacgca ggccggctga
     4381 gcttcttgat gtatgcagaa aaaggatctg gaagaatcaa ttccatatct cgagtgagat
     4441 gcgaatgcag tacaattgct tctacactta cgtcagtcaa ttccagaaac actctgtccg
     4501 ggcgaaaacc agtaatgaaa ccgcgaaagc ttttttgtcc gcttctctca atggatcgaa
     4561 gcatcttcag tttccagata tcgcgctctg cgtccatcgc cttgcgttcc atctcgctag
     4621 tatgaaaccc gagttcagaa atttcagctt ctgtgtatgc atgcttcttt ttctggatca
     4681 ggttttgaag agcgcgatgc acaacaagat ccggatacct ccgaatagga gaggtgaagt
     4741 gacagtagtc gcgaaagccc aggccgtagt gacccagagg ctctcccatg tactttgcct
     4801 gcatgaagga tctgagcatc aacatctgaa agatgcgatc gggcggcaca ccttccagtt
     4861 tgcgcgtcga tcctttctgt acgctttttc ctttctctgt gaattcatga acaaccttca
     4921 gcgcatgatt gatattctcc ggatctgaat tcggaagttc tagattgatt ccatagatct
     4981 tgaggaagtg attgagcgct tccagcttca tgtcgtccat tggttcgtga acgcgaaaca
     5041 gtgtatgcgc ttccttcttt ctctggaatt cagcggtgca gatgttcgca gaaagcatgc
     5101 attcttcaat taacatgctg gacttcaacc gctcgcgata cagtacttct tttattctac
     5161 cttctgcatc atagatcact tttggctcgc gcatatcaaa gtcgatgcgc cctgctttca
     5221 atcgcgcagc cttctgtgcc ttcgcaagcg accagactcg cccaagcatg ctgtcggatt
     5281 catccagcat ggtttctgcc agatcgtagg tgagccgctt gtccacgcgt atcacggact
     5341 tgtagatctg atactttaga atctcccctg tgtctggacg gacgtccatt tcgcaggtga
     5401 atgccagtct gttcctttca gcgatgagcg aacaaaagtt ttctgaaaga acaggaggca
     5461 acattggtac cactcgattg gcaagataca ccgaggtagc tcgacgcgcc gcttcacgat
     5521 ccaggtctgt tccaatcctt acgtaatgcg aaacatctgc tatatgaaca taaagtttcc
     5581 agagccgcgg agactctacc ttcagactga tcgcatcatc aaagtccttc gagtcaggcc
     5641 cgtcgatggt gaccgtgtaa agatcccgta aatcaatgcg tgaattccaa tcaaagacat
     5701 tctccggttt tacttctgaa gcttcctgat catattccgg taaaccttct acttctggat
     5761 acaccggatc gagattgtgc ttcatgagaa cacgcgcata atccggatcc atatccgtat
     5821 cgctttcaaa ccgaacaaac tcagcttccg gcaaaggtag acccatgtgc atgatggtct
     5881 tcccaggcat gcgaaccaca acgaccacat cttgcttcat cctgcttacc agatccgcag
     5941 gtagtcgcgg ggtcgccaaa caggccgtta tgcgagagtg tatatcaaga agaacgccag
     6001 gaattccgcg atgaactggt tcgctgagca acttcattcg ataaagctca cgcgctttct
     6061 cccgaatctc aatgacctct ccttcgaatc gatctctggt tttgtctgtg agcttcacga
     6121 gaacaagatc gccggtcagt gcattcatgc ttttggctgc cggaatgaac aaatcacgag
     6181 acgcaggatc tgcccctctc acggcaccaa agaccgcgcc agaaggagaa agagatgctt
     6241 tggctacggc cgcaaaggga ctcgcaggaa tgatcttatt gccatcggtt cgtaccaggt
     6301 tcagtagctt tagttcggcg agcagtacgt cgacttcgga ttcggcctgg taacccctta
     6361 cctgccgact ttttctaaaa gattttgctt ttgctttgaa cgcgcccgtg ctgctaaaag
     6421 cagacaagac ttctttgcga gggagcttac gccctgcatt ttgttccagg tatcgcaaca
     6481 atcgcgatgc tgtactcatt ttatattaaa tcctccagga tacctggcct cggccggatc
     6541 cttacgtaaa tagtagggga acaattcctg ataggagcct gacgccggca tctccgaatg
     6601 cgcggcaaaa atccatgccg cctccggctc cttcagctga cgcgaatctc ctccgttgcg
     6661 agcgcgaaac gtatccgggt catcacaata caccggcaaa cctgacgcgg caacttcctc
     6721 tgcggttaaa tcctgtacgg gaatcagatc catttgcgta gcgctgagtc cagactctgg
     6781 aattatcttt gtataaaaac ggttttgttt tccgtcaatg cacaccgcga agggacctgg
     6841 ctccatattt cgaatcggca gcgaatagca cgtcaaagtt ccgatccctc ttacaggtac
     6901 attccagagt tgcgccagat ctcttgctgc gctcacgcaa atgcgaatcc ctgtgaatga
     6961 tccaggaccc gtaagagtca caatctctga aggtcgctta acgttagcgg cagataagac
     7021 ttcctggatc gcaggaaaaa gattctggaa cgcttcgcgc ggagcttttc gcacgatctg
     7081 atattgattc tgttttgaaa aaagaccgac tacaatgtgg gtgtgagtgg tatcaatgca
     7141 aagacagtaa ttgttcaccc tgccttctat agaactgaat gtgcctgagg ctgtcatcct
     7201 tttcatcaat ggaaatttcg accagaaata cgggaactcc gggcccaata tgaggtagag
     7261 cgcgatctcc ccactctatg gcgtgcacgg ttggagcgtg atcgacgcga tgccagtctt
     7321 ccagaaatcc caactcttcg agctctgcga ctttctgcaa tcggtacaag tcgtaatgat
     7381 gaagcactcc gcgcgaccct tcatatcgat tgtgcaaatt aaatgtagcg ctattgatat
     7441 tctcgcgaat cgacatagct cgcatgaatc cgcggcaaaa ggtggtcttt ccagcaccag
     7501 gctctcctcg caacaagacc acacttactg gaaggctttc gaggaattgc gcgaagaaag
     7561 cgcagcaggc ttcctccatc tgcagtgcag aatcaatttt ccgtaaactc atcaaagact
     7621 cggtcaacga cgccaatcat cgcggtcgct taaatcgcgg ccggagagat tcttataaac
     7681 gactgctccc gctccccaga aggaaaccag cacaactccc gcaaaaatcc caatccactt
     7741 caggaggaaa ataccagtga tcacagaagg aatctggacg agctcaggct ggaatcgctc
     7801 cattatgtcg cgtgccacac ccgcaaatat gaaaaaaaag acagagaaga caatcgtata
     7861 gaggaccttt cgggcagctc gcatggatca gattcgtcag agaatcgaca aaaacaactc
     7921 aaaaccatag atcggggccg ccgaccaacc gatgtaccgt ctgggggttt gtttgacaaa
     7981 aaatgaatcc gagtttgtta tcattattat agcggagcca cggattggcc cgtctgattg
     8041 taccggggaa ggtgtatgaa aaagtatctt gggataggac tcattctggc tgtgagcagc
     8101 atggctcctc tccgcgcgca aatcgtttgt acgggaccgg gatgccagta cctccccctg
     8161 agccaggcaa ccctggatca gatgtattac gattttaaga cgcaatacac ggaccagctc
     8221 ttcaaggaca tggctgcggc aggagtgctt gcaaacctga ctggagcacc caccggaacg
     8281 gtgaatctct acggctggac cattggagcc agcgcaggac tgggttacat cccgataaaa
     8341 gaagtcagca ttccaatcac aggctacggc gaactcaaag acatcccgcg cgctggcggc
     8401 gcagtcaacc ctcgtatctt ttttggagcg aatgtaggct ggctcctcaa ccaggcgtat
     8461 gatccgtggg gtgatggaga atcctctgag ccatttttcc tctccctctc tcgattcgac
     8521 ttgtacggct cattcatcca ggaccgaagg cactatgtga tgcaagatag cgggcaagtt
     8581 actgtaaaca cccgtaatct cggatttgat gtgcgatatc atcttgtaga aggcaaacct
     8641 ctggcagccg gtccacttct tcgcttcctc ggcgtgtctg ttggtgctgg ttatcacacg
     8701 gccgcgatgg acatcaccgc catacagaat cagaatcaga aaatgaaaat caccctgaca
     8761 ggtggttcca atttgatctg ggacggcgcc aatcagggga agatacaaac ccaggtaaaa
     8821 tccatgccgc ttgaaatcat gactggtctg caaattctct atgcgttgaa catgtcgttt
     8881 ggtggaggaa tgagcatcaa tcgtggatca gcagatgtga cactgactcg actcggtcct
     8941 gtttatctgt ctaacgactc agcagcactg cttgcggccg ccaccgggca aacagtcgcc
     9001 tcttccgcgt ataattccta tctgacaatg caagtaacgg gaagtgggca ggctccgcgt
     9061 aacatggcgt atgcgaaact gggactggac ttcaacattc tctggtttaa gattttcgca
     9121 gaaggtctgt gggctaaacg cgcttacggt gcaaaccttg gcgttcgctg gcaaatgtag
     9181 gatctacctg gaaagaagcg gctggagaag gcgtccgaaa gtctcgtagc ccttgtcatt
     9241 gaaatggacc tggtccacct ttccgcctgg aaggcgaaag taaaattctt ccttgagttg
     9301 cggcttgtct gaatcagaaa gagcaggcca taaatccaga tactcaatcc cttcgtcccg
     9361 cgtcaggtac tccagcttgc gattgtagta gggagtgatg ctgtttgctt tccacgatac
     9421 taccggtggt atgctgacca tgatgatcct tacgccgggc aatgattcct tcaaggtctt
     9481 gaaaatgctt gtggtgtttg cgagaaccgt acgcatgcat ctgccctgca gtatgtcatt
     9541 tccgccaatc actatcacaa ccgtggaagg atgaagggcc accacatccg tatccaaacg
     9601 cgcgagcaat ccacccgatg tatctccacc aatgccgcgg ttagcgacat ccaatcctgg
     9661 cagatacgct tgcagacgag gtcccgcgaa aagggcgatg gtggaatcac cggtcaaaac
     9721 aatgcgcgca ctctttctgt tctcgttgcc ccagttctgc ctgacagcct gcttgatgag
     9781 cccaaatcca ggatcgactt caacgggcag aggcgtctct gtgcatttga attcgcgctc
     9841 caggtaatcc tggcccatag aattgccacg aggctccgct actctgcagg cggagacaac
     9901 gaccagcaga atggcgacta acgttaagcg cttaaaaacg aatgtcatga tacgatgatg
     9961 ctgcccggtt ttgccggctc cctgaatatg cgaagtcctt tgagttggcg ctgcctgcgc
    10021 tttcgaatga atcttttgaa gatcgccttg atgaaatttc tgcgtctctt tttcatcccg
    10081 tattcgtcca ggaagtcgcg cacaagagga tacacatcat cgcgcgcacg atcccccaga
    10141 acgattcctg tgtgacagta gtcgcaatgg aagccggttg cttttccaag aagtctaaat
    10201 tccttgatct tacttccgca ttgttcgtaa gcaaacctga cagattctgc aggagcaatg
    10261 tgatcacgag taccggccag gaagagtgca ggcgctttta tgtttttaaa tccgtctctg
    10321 taactgtgtg aaccatcccg agtgtgctcc actccactct tgatccagtc gaggaactgc
    10381 gagatgagtc ctgctccaat attctcaatc ccgttcttca tcaatctgcg aatggttact
    10441 ttcgaaatat tttctgaatc atatacaatg ttgtcaatgg gagtcagaaa cctgcctgtg
    10501 agcggggaga tgatctgtgc accaaacttc atatcaagca ctcgaggaag ccacggcatg
    10561 ttcgcaatcg caccccagat ggtcgtcttc gcatgatcaa tcttcccggg actggccagt
    10621 gctgtgaaac aggcaatgtc ttctgaacca ggaagttctc tgcccagaaa tccgtacata
    10681 atcatggatc ccatgctatg acccacccag tttactttgg gtgcaccggt cagttctttg
    10741 accctgcgaa tcactgcagg cacatcatac ataactatgt catcaaaatt gaaatcgcgg
    10801 tatctgcctt tggaagaatg atacgacttt ccgcatccgc gcaaactcaa cacgaacact
    10861 ggatatccat tctgtttcag aaagtatgga aggctgtgcg cttcgtccag atccactccg
    10921 tatttgttca ccgcaatgcc gtgagacatg atcacaggat aggcgtttgc tttggggtag
    10981 cgcggcatgt gaaaatgcat cgctatgttc cagccatcgt ccgtgtgcgc gtaaaagagc
    11041 tgatcggcaa tgtcccgcgt gccgtagagt ttatcccacc agtagaggaa gaacggataa
    11101 aataggacca ggacggccag aaacgccgcc atgacgagcg cagctgtgcc cgcgagaaat
    11161 gcaaaaagca aaatgatgat gatcgagacc aggatggtca gcggatcttt gcgttttttg
    11221 atgggaccaa acaaggtgct ttacaaaatg cgtcgagagt gcccttgagc aactaaaata
    11281 tgcagagcga aagcggcggt taccagacct ttcagggtcc ctttttctcc attcggctcc
    11341 catctcactg ggaaatcgaa gtgatcgaga atatgcccgc gttttttgac ccggaaggca
    11401 atggagtgct gcaggttgcg gccttgaaaa gacagggtca ggattatgat atccaggcag
    11461 aactcgagcg atatctggcg caaaatcaaa ttgaagcaga tccggagcga atgtccaggt
    11521 ttcgcctgcc atccggcctg aacggaattg cagttgagta catcgtggat aaccgattct
    11581 ggatggtcaa cgcgttgggc gcggggaatc aatgcttgct tgcaatttat aactcagatg
    11641 aagtaccgga ccagggcatg gccatgctaa tatcggaagc tcttgcttcc atagagttca
    11701 tgcattcatg aaacggattg tggtacttgc ttctggtcgt ggatccaact ttcgcgcagt
    11761 tctttcggcc atcgaggatc atcgcatcgc aggcgcgcac atcctcgctc tggtctctga
    11821 tcgatctgga acaggagcag aacagatcgc gcgcgatgcg aacattccca tcactcttgt
    11881 agattattca gacaaatcga agcgcgcgtc ctcggacgaa aatctaacaa aagtcatctc
    11941 gaatctcgcg ccggattgcg ttcttgcact cgggtttatg cgaatcctcc cagaaaaatt
    12001 ggttcttgcc tttcgtcaca aaatcatcaa tgtacaccct tcccttcttc ctgcttttcc
    12061 aggaatgaat gcgcaaaagc aggcgctcga atacggcgtt cgcgtaacgg gagcaacggt
    12121 gcattttgtg gacactggag tggatacagg cccaatcatt ctacaatctg cggttcaagt
    12181 tcccgcaggg gcaactctgg aagaaatcac ggacctcatt caggcagagg aacatcgcat
    12241 cgtagtgcag gctctggatc tttttctcaa ggatcaaatc caggtggacg gcagaaaggt
    12301 actcataaaa taaccaggaa aacagcatga ttcagatcaa aagagcgctc atctcagttt
    12361 cagacaaaac cggcctaaac gatttcgcaa gattcctcgc aggaagaggc gtagacatca
    12421 tatcgacagg agggacactg aagactctgc aggaagcagg gatcaaagcg agggcggtgg
    12481 aagaggtcac cggttttcct gaaatcatgg acggaagagt aaaaactctt cacccaaaga
    12541 ttcacggcgg ccttctctct ttgcgagaca aacaatcgca cctggacgca atgaaagcgc
    12601 agcaaataga agggattgat cttctgattg tgaatcttta tccatttgcc gcgaccatcg
    12661 cacgcgacgc gtcttttgaa gattccattg aaaacataga cattggtgga ccggccatgc
    12721 tgcgcgccgc agccaagaac tatcgctttt gcgcggcagt aacggaccct tctgactatt
    12781 ccagactcaa agaggaaatc gaaagagcgg gcggtattcc tgagaagatg tccttgaatc
    12841 tcgcgcgacg cgttttccat cacacagcag aatatgattc gctgatttct cagtacctcg
    12901 atcagatcga agcagtaaaa tttccagact cacttacaat tgcatttcgc aaggtgcaac
    12961 caatgcgcta cggcgaaaac ccgcatcaag aagctgccta ctatcgtcct ctgatcgaca
    13021 tagaacgcga aagaagaacc ggtggcctgt ggcatcaaat tcaaggcaaa gaactgtcgt
    13081 ataacaacat gttagacgcg aacgcagctc tgatgagcgc cctttccctc ccgggacatg
    13141 gagttgtcat cgtaaagcat ttgaatcctt gcggagtcgc tcttgcatct atggatccgg
    13201 cgctcggtat ctttggcgca actccggggc aggaaggatc ggcaaacgtt cagacttttc
    13261 aaggtggaat gccagaagcg tttcgccttg cgcgcgcctg cgatccagtc agcgcgtttg
    13321 gcggagtgat cgctcttacg gatctggtgg atctggaaac agcaaatctg atcaatgaga
    13381 attttgcgga agtgatcatc gcgccagggt actctccaga agctctggat gcgttttccg
    13441 cgaagaaaaa tctaaggctc ctcgaaatca agagaccaga gcgattcctc gctccgggga
    13501 tggagcttcg cctgaatgaa ctcgggctac tctatgaaga catggatcac acctacgcac
    13561 ccagatctac ctggaaggtc gttacgaatc gggcgccgga agccgcagaa ctcaatgccc
    13621 tggagcttgc ctggagagtt gtgaagcacg taaaatccaa cgcgatcgtc tttacgaatg
    13681 aaaggtccac acttggaatt ggggcgggtc agatgagtcg ggtcgattca actgaaattg
    13741 ctgcctccaa agctaaaaag gcggggcttt ctctggccac gtcctgtgtt gcttcggatg
    13801 cattctttcc tttccgtgac ggagtggacg ctctggctcg cgcgggagca cgcgccgtga
    13861 tccaaccggg tggatctgtg agagacgaag aagtgattca ggcttgcaac gaacacggca
    13921 ttgctatggt atttaccggg atgagacatt tccgacattg atatggaccc gcataagcga
    13981 tgcctgttag atacaggtct gcaccagaag tgcctggcaa attgcaaatc tgcaattcag
    14041 gacaacaggt ggcagtccaa tctcatgaac tcgtgcatcg cgctacaaaa gctggaactc
    14101 ttccaacaag gtaaaaattc attcggtgta gcacaaagtg cgttcctcca gggagacgca
    14161 aagctcatag atcaattcat ttctcagtcg gaggccaacg tagacctcgc gcgctatttt
    14221 cttggcgtac agaaaccaga agattccatc agcattgcaa gctccctgaa agcggatgct
    14281 ctctatataa tattcaaacg cgactacgca gtttttctgc aaaagcaaaa acaaaaggca
    14341 cctgcagacc gcgagaccaa caactacttc ttacagaaag ccacccagta ctggtccaaa
    14401 cttcccgtcg ccaaaatctg tacggtcatt gcctacatcg tgcgagagaa aaaagacgta
    14461 agtagtgcgg cgcaatttat ggtgcttatt ccgtccagcg tcatctcccg ctttccggaa
    14521 ctctccggct tgacagaaga tgaagaaagg gaactattcc tggggctgga gggagatctt
    14581 ttcaccttac cgctgttatc ccctggcata tacgatcaca tgatgaccct gtttgcagaa
    14641 cagccggaaa tttccatggt gctcgcggga atggaagagc tcgtaaaaag acgatccgtt
    14701 gtgatatcaa ctacagacaa tatggtggag tattacaaca aaaccaattc acgactttcc
    14761 cttcctgccc tctattccga actatttgga attgaattcg aactcgcggc ggaagtgctt
    14821 catcatctgg tggaacatca ggtcatcacg acaaacgaaa gaaatactct gctggaattt
    14881 ttgaaatcgg gcaagctcga cttcatccga caaatgagag aagacatttt gtccgtctca
    14941 ccgcttccgc gcacagacta aagtgcgaat ttttttcgaa ggtcgtctat ctgtggtttg
    15001 aactggttcc acatcgtagc tggattgtgc tgaaacgtag catacacacc ctggcggcat
    15061 agatccacaa tttcactcac cgtaaaattc aacgacttgt ggaatttgaa atactcatct
    15121 gaaagagtga cgtgaaagat ctccgggtca tccgtattca ccgtgcaaat gatcccttct
    15181 tcatagtaac gacggacagg atgatctttc tcacgacgaa catatttgcc tgtgaagata
    15241 ttgctggtca ggcaaatctc aataggaatt gccttcttac gaatcaattc aagcagtgct
    15301 ggatcttgaa ctgcggatgt gccatgtccg attctctcag ctttcaaaag ttctacagtg
    15361 tcccgaatgg accatgggcc gtcatcctca ccggcgtgcg ctaccgtgcg aagcccttcg
    15421 gcctgggcct gtgcgaagac atctctgtaa tcacgcgccg ggcccatgag ctctgctccg
    15481 cccagcccaa ttccaatgat agaagggcgc ggattggaca atactctttg caggttttta
    15541 ctcgcgtttt ctgggccaaa tgtgcgcgaa acgtcaatga ggaaacgaac gtccggccct
    15601 ccgctgtttc gacattctcg cgaaagcgaa tccagtgagt ccgcaatgtc ccggtaatcc
    15661 aatccatttt gaatcattcg cgagggagca agaaatactt ccgcataacg aatgttattc
    15721 gattcaagat actgcctgag attcttgaag atcaatgcga agtcttcggc catcttgata
    15781 gagtcgataa tgaaaagaaa gagtttgatg aactcctgca aattcgtaaa ttgataaagc
    15841 ttctttagtt gttcggattc gtatggaaca ccattcttgt ccagcatgcc ctgaagcgtc
    15901 tgctgattga tgcaggcttc catatgcagg tggatttcag tcttgggcat ggcccgaatg
    15961 aattccatga ttttcttgtc ttcggaagtg agctcgtatt ctttcttttc gcggtcgacc
    16021 gtaattcgcg acgcagtagc aacgtcaacg gaagcaatgt cattgaggag gtcttcgggg
    16081 atatgttcta cttcaagttc ttcgagttga attttctcat tgagcaattc attgatatgg
    16141 cgatcaaacg ccacttgcat tccggaagta aacgctctgt tggcgggtag acgggcccgg
    16201 agacggcgga gctcttcgac gtcgcgatcg agcgtgcgaa ttctctgcaa aacatcgtcg
    16261 agtcgcatta ccatggttat accttgaagt cctggagagc ttcttcaatg aatccaatca
    16321 actgacgcat ctggtcaagc tgatctttgt ccttcacgtc cggggacgcg gccaggcgat
    16381 ctactgtttc gcgagcgtat ctgcagtatt ggtggattcg cttcacgcga tcctgtaaag
    16441 acatgaacgt ccgattcaaa tgagccgcgt gttcctggag ctcatcatcc tttcgcagat
    16501 gtactctggt ggaaaggtca ccggctgcaa tggccgcgag agtcttttca agatgaacga
    16561 gtggaccggc gagcctggaa gaatagagca tcagactcca gccaacaccc acgagagatg
    16621 ctacaaagag cagagccact ccgccaatcc ccagccagcg aagttcgtcc atggaatact
    16681 catgacgacc ggtggttgca gaccagatca caaccatggc ggccgcggac aacacggcaa
    16741 gaggaagcat ggcccgacgc accaggcgaa gctgaaattt ataccggagc gatgtccgtc
    16801 ttgcgctgcg cttctgctcc atgtcaggag atgacggtca acaggctctg cgcctgtcgg
    16861 ttaccggggt caagttgcag tgcccgatcc aggagttcct gagcacgcga cgcatttccc
    16921 agcttctgat acagatccgc cat
//
LOCUS       DAORFF010000009        16307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1461_length_16307_cov_4.351280, whole genome shotgun sequence.
ACCESSION   DAORFF010000009 DAORFF010000000
VERSION     DAORFF010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 16307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16307
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1461_length_16307_cov_4.351280"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1799)
                     /locus_tag="PKK76_00790"
     CDS             complement(93..1799)
                     /locus_tag="PKK76_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNL67026.1"
                     /translation="MITYRYGPYEPERDRPWDMDRLMNVLSDMIMRYDLELDEALRML
                     IDRGLPVNMFLKEGGMDDLVHSLQQQLQQKIDDVLGQFDIKPARKDTEFDLKKDSGDL
                     LNTAKSKEWKDRIEDAIRNLSIDELYRIKNEVMQEKKRPVSMDDIASVIQDMEDLDDL
                     VAGEKKYKFTGSTPLSRDEAIDILKHLDDLEKLMQALKEAEESGDLFNFNLEKLAQYL
                     GPEQFEEFLEKREQIFEKLRELLEKQGRIVPDPETGEMRLSPESVRKIGRRALEEIFS
                     KLKADTSGGMHSADETGESENVSSKTRPFDFGDPVSNLDLSSSIVNGYVRRKSSKPDL
                     SDMEVFEARGSARSSTVVLIDMSGSMMRSDRFYNAKRVVLALDALIRQDFKDDRLTIV
                     GFGTLASVIPPAKVPSLQPHPVTMYDPHIRLRYNMAAQTEKGKDRIPKYFTNLQRGLQ
                     LSRTLLGSGETKNKQIILITDGVPTAHFEGSSLHVNYPPSPADFEFALREVRNCTQDN
                     IVINTFLLTSDWEMGYFGEESFMRQFMKQSQGRVFYPHPTQMGKMVLVDFLQNKKAIF
                     QY"
     gene            1890..2603
                     /locus_tag="PKK76_00795"
     CDS             1890..2603
                     /locus_tag="PKK76_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNL67027.1"
                     /translation="MELTFLTYNIHRAIGFDRKYRLERIIETCRDVDADIVALQEVDQ
                     FAPRSKGEDLAHLIAQNLDMHYELGLNVRLQKGHYGNATFSRYPIEQAHNFNITWGIK
                     KKRGCLITSIGLPHAKIAIFNFHMGLARMEQVRQKKKIVHSDHMERLARVPMVLLGDT
                     NDRTTRLSHSFLESGFRDTCPGKGHNTFPAYAPILRLDRIFVTQQWEVVEHRVVRNKL
                     TRIASDHLPVVARLRLKSY"
     gene            complement(2609..3502)
                     /locus_tag="PKK76_00800"
     CDS             complement(2609..3502)
                     /locus_tag="PKK76_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67028.1"
                     /translation="MSTQFDLSHLKPEEIEKVRKVLLNLIAGIKARQVITAHGSALLK
                     DEEITLQSLLHPERGDVLVTIVRDHGMLHVFLSNRRDPGSPFSVMDYQTMRRFPARRP
                     VDSHHIVKDGETALFLCTQQDRDMLARESPDIISAYSSHFDVKEISELKTQAAPVDPI
                     SKSIQEKWESYRRHDPGDPAVAHIEKELFGYTITYAKYKASAREETVIIRPEKEKLFV
                     GQLIRDIYPYNIRAMFRKDNHEQHLQKLGEVHSTLRKLREQVTNRKGDSLPEPYMHYL
                     TGLQEMISNVLEKDPQLNGFV"
     gene            complement(3486..3851)
                     /locus_tag="PKK76_00805"
     CDS             complement(3486..3851)
                     /locus_tag="PKK76_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67029.1"
                     /translation="MLTFVTIAALALALIVFLNALNLPTSAGAKKSPANIEKPVTLDT
                     ARILRMKAAERPRICPVCGTPLSQDEYLIAAIFPDLGPNQKRQAHIYGCRHCYVSGGV
                     NLTRVHPETLAEQPNVHSV"
     gene            complement(3925..4254)
                     /locus_tag="PKK76_00810"
     CDS             complement(3925..4254)
                     /locus_tag="PKK76_00810"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNL67030.1"
                     /translation="MNNPLDEEQAPGVPQSAQLRELKNAFVRSLPFDEGTRELMLLGV
                     SWDEIAGNLSARTRPEKIENEALYVSVAGGVYMQEFSFQSKTILKKVQNLIPSIRRIM
                     IVQSRSG"
     gene            complement(4235..5344)
                     /gene="recF"
                     /locus_tag="PKK76_00815"
     CDS             complement(4235..5344)
                     /gene="recF"
                     /locus_tag="PKK76_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNL67031.1"
                     /translation="MRIEQLLLKNFRNYESLSILFPSRLTFLLGRNAAGKTNLLEAIG
                     MLSHGKSFRGASDHDLLRYGSNGYYIKAKYSRAGQKGEMEIATDVSTGDLRRKIKFND
                     KAASSRSSIVGEFICVVFSPSDLQIIEGSPADRRKFLDAAISSMEPEYLQNLIGYQKA
                     LRQRNALLKKIRQRQANIADLQIWNARLVQFASQLWSRRENFLLSFKEPFRDALEQIS
                     GNRDAMNLELQFSHRDDLKESYDKNVVRDLSLGYTTSGPHRDSLVFLKDGRDIMQFGS
                     QGQKRTAALALRIAQFHHLKQKLKITPLLLIDDVIRELDTMRRSAFVKLLRESGQAIF
                     TTPDLDGIDEDLKSLVSQSSVIHILGDGRVEQSVG"
     gene            complement(5354..6466)
                     /gene="dnaN"
                     /locus_tag="PKK76_00820"
     CDS             complement(5354..6466)
                     /gene="dnaN"
                     /locus_tag="PKK76_00820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNL67032.1"
                     /translation="MKFTVDRQQFLKAITTVDGIIPSREIRSVYSNVLIEADKGEAVF
                     TTTDLEIALKTTLPITLDKKGGTTVPAKKLTQVIGAMDGETIEISLDDDKLIISSPKA
                     RTKPRVNLVTSPTSDYPPTPSPSSEDLISFSPAVFREMILKTYYSMAQDDSRYVFNGL
                     FLKSQKNSNIVVATDGRRLSKISRDFPSALPFKDGVIIPAKAIKELVRLLDAGEEAMI
                     AYDDKDKRVHFRIGRVDLVTKLIDGAFPDYEQVIPRKLDYKIRINRIDLEKAIKQAAS
                     VAIEPARQVRFIFSEGTVGFQASTPDLGGSESSLECEYSGPEMVIGFNSVYLMDVLKV
                     IPGEDVILGFSSPSSPCTVGDPADPDFLSVVMPMKI"
     gene            complement(6707..7951)
                     /locus_tag="PKK76_00825"
     CDS             complement(6707..7951)
                     /locus_tag="PKK76_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HNL67033.1"
                     /translation="MIKDAWNQVLKDFAGESSQNSSLKWLYSAKPGTSTDSSRLILNV
                     PDNGILSHLREYTRPIEVKMASILGRPVRVEFQLSHSLPEWIHPEYSFETFLESKCNS
                     NALQCIRAACRQPGRHNPIFLSGPANSGKTHLLHSYARSVIEAKPGARIAYLSGIAFK
                     LGHLRAIASRSSLDFKESMMSFDCVIFDDIHLIGSGKSTLEEIDFLITAFLDRGKQIV
                     CSSSTEPGSVFPSGRLRSRILSGIVVQMESVDADLRRAFCQKMAEVNPNILDHSTIQR
                     ICKSATDIREIKSIFGKIELQSRESKHLSTSNIDSVIEGTLRRPAIPSIHAILEAVTR
                     HYHVSKQDILSSSRKTEHTWPRHVAMYLALTQGGMSKSAIARFFKKKDHTTVVHAEKK
                     IQIALENDDALAGALKAIVSTL"
     gene            8145..8300
                     /gene="rpmH"
                     /locus_tag="PKK76_00830"
     CDS             8145..8300
                     /gene="rpmH"
                     /locus_tag="PKK76_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNL67034.1"
                     /translation="MKRTFQPSNKRKNKTHGFRSRMATANGRKVLSNRRKKGRKRLTV
                     SDQTHRK"
     gene            8636..8875
                     /gene="yidD"
                     /locus_tag="PKK76_00835"
     CDS             8636..8875
                     /gene="yidD"
                     /locus_tag="PKK76_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNL67035.1"
                     /translation="MNANTLAIAGIRVYQNTISKLIPGRCRFYPSCSSYAVECFERFS
                     FGHAFIKSGYRILRCNPFNEGYFDPVIPDEPQNQA"
     gene            8881..10788
                     /gene="yidC"
                     /locus_tag="PKK76_00840"
     CDS             8881..10788
                     /gene="yidC"
                     /locus_tag="PKK76_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNL67036.1"
                     /translation="MENKEEQKPGLGSFLPVLLIGIAVLLFFQFSGSGSHPPVTQEHE
                     GTGAKLTDFEFQSGPGEIVSVDTGNFIVKLNSQGGRIDKFYVKKSKELNVPDSVLKTE
                     TPEAQSYQAFEGSRGNGFDFQFHGYYYGDGASQLGDPALNKALFKKEGPFQDKDGVVE
                     VRFRLPVRLRGQNLEIVKIYRFLKGEHFFRQIAVIRNLDKKEFVWGGPMFFRPFGDVG
                     PSTEMAGGINPALHGRFYYYNGEFKSRMNTPPTNSILAPIGCGENLKDKPYSVYAEHP
                     NSLHFVGAHSKYFFAYAKFLDTNNPQSQVDGVAIRNEIDPDGKRAFTTILNDFRLAPN
                     TGELELQPAGAQTTGGHEYIKGIQQRNDAVILDQEIYYGVRTDESHAFANEALARTEF
                     GSSDTDKEARGVIYTSSFLALFSKVRDAIVWVMRFLHVYLGNYGWVIIVIAVSFKLIT
                     FPLNQMQAKSMKRMSALKPELEKIQEKYADNPAEKQKRTMEVYKKHNVNPAKGCVPIV
                     IQIPVFIALYSAFSESIELWRSPFILWMTDLSAPDTIYTIKDFLILKDFHINVLPLFM
                     VASQLLQQRLTTVITDPQQKIMMYMMPVIMIFFFWTMPSGVTLYWTVQNVLAIVWQLV
                     TNRMSDDGKTA"
     gene            10809..11573
                     /gene="jag"
                     /locus_tag="PKK76_00845"
     CDS             10809..11573
                     /gene="jag"
                     /locus_tag="PKK76_00845"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNL67037.1"
                     /translation="MNYIYEAEGKTRAEAEQIALSALGLKEEDVTFEAVSAGKSFLGI
                     VNRKPTVVRLFAKNVPIETVIKGTVITLIRKMGLTVENVGVHIEEESFVVSIDSEDSA
                     ILIGKQGRTLDSLQFLTNCMVDSRIRNNRRVVLDVAEYRERRKQRLTKLAKAVADRVA
                     RTSQSVLLEWMNPYERRIVHLALEPDERVTTRSDGNGVYKRVRVLPQKGRPGSGPQDR
                     QDRPDRQDRERNFNRAPREEEEEDQDFNRKDAFDDQ"
     gene            11570..12898
                     /gene="mnmE"
                     /locus_tag="PKK76_00850"
     CDS             11570..12898
                     /gene="mnmE"
                     /locus_tag="PKK76_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNL67038.1"
                     /translation="MTIAAIATYPGHAALGTIRVSGPGTLDIISRFVVAPDLKPIPFS
                     NQPRHSFFAKFMDANVLVDEVVAVYFKGPHSYTGEDSAEITFHGNPILLKSALRLLVE
                     SGIRLAEPGEFTSRAFRNGRLDLTRAESVKRLIEAKSEYELSAARAIYGGELSRRMSI
                     LRSAMIGLKAETEAEVDFSTEDLTFQSREERRARVMGLIAELDSLLARGSATGRIREG
                     ISIAIVGVPNAGKSSLLNRLLGYDRAIVTEVAGTTRDTLSEELQIAGIPVRLVDTAGI
                     RETNDIVEREGVRRSQRELEESTLVIHVIDGSRESYASIVESDRDNVIHVINKADRLH
                     PGANPGSAIQVSCLTGAGMDHVEEAIRDRVLERPESRDPILLEERHRFHLLAMKATLT
                     KIMELWQQNAPDEITALEIDDVIDHIGSITGRISNEEILGRIFSTFCVGK"
     gene            12959..13957
                     /gene="gap"
                     /locus_tag="PKK76_00855"
     CDS             12959..13957
                     /gene="gap"
                     /locus_tag="PKK76_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNL67039.1"
                     /translation="MLKIGINGFGRIGRLAFRTALERQDIEIVGINDLLDADYMAYML
                     KYDSTHRAFKGTVEAKDGQLIVNGKKVRVTAEKDPANLKWGDIGAEVVVESTGLFTTI
                     EKAQAHIKAGAKRVVISAPSADAPMFVMGVNHKTYKADMTIVSNASCTTNCLAPLAKV
                     VNDRWGILEGLMTTVHAVTATQKTVDGPSHKDWRGGRGAYQNIIPSSTGAAKAVGKVI
                     PDLNGKLTGMSFRVPVADVSVVDLTCRLSKPAKYDDIKKAMKEASEGELKGILGYTED
                     EVVSQDFLGDPRTSIFDAGAGIQLSDTFVKLVSWYDNEWGYSNKLVDLIQHISTVK"
     gene            13986..15182
                     /locus_tag="PKK76_00860"
     CDS             13986..15182
                     /locus_tag="PKK76_00860"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNL67040.1"
                     /translation="MNKLTVKDLNVAGKKVLVRVDFNVPLKNGVVENDKRLRASVPTV
                     QYLISNGARVILMSHLGRPKGKPVDSMRLGPVADAFGKLLGKPVKSIGTCVGDEAQAA
                     ASALKDGEVLLLENLRFHPEEEGNDPGFAKQLASLGDVYVNDAFGTAHRAHASTEGIT
                     HHVKQSASGFLLQKELDYLGGAVNNPKRPFVAILGGSKVSGKIDVIEALLPKVDFLLI
                     GGGMAYTFYKAQGLEIGTSLCEEDRIDMAKTLLAKAGTKLLLPVDCVISDAFDFDARK
                     VGTLKTVAANGIPKGWSGLDAGPETISLFKDKVLSAGTVLWNGPLGVFEIAETAVGTF
                     KIAEALAEATKKGAITIIGGGDSAAAIEKAGLEESVSHVSTGGGASLEFLEGKDLPGV
                     SALTNR"
     gene            15179..16144
                     /locus_tag="PKK76_00865"
     CDS             15179..16144
                     /locus_tag="PKK76_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNL67041.1"
                     /translation="MSVLRGLRRLNRYEARLFRIARKAGKLAQIEQLGYSARSSDGFR
                     FPIYSLTIGKPAALERSPVGLVAGVHGLETIGIRVLLDFLESIFDEPNRDVSQALLDG
                     DIGIVSIPILNPGGVALKSRANPAGVDLMRNSGKDGDTALPFFGGHRMSSKLPYFRGK
                     SLQPESRALFRFCLERFSQARKGTILPVLDVHSGFGMKDSVWWPYAGKKTPSADHYLF
                     ERLSRILHTEGDGITYEPQSQAYTVHGDLWDRLYDDYRRSKKPAKLLPFTLEIGTWSD
                     VKRNPGRLLSKRGLFNPPPEQKALMIQRYRPFLSKFVLLSLLYGE"
BASE COUNT         4257 a         3813 c         3918 g         4319 t
ORIGIN      
        1 ccgagccctt ccagtttttt ttatcgccgc acctacgttt tttcacaaag ggtggggagt
       61 gaggctcatg caacggaaag gaacggcaac cctcagtatt gaaaaatggc ctttttgttc
      121 tgtagaaaat ccacgagaac catcttgccc atctgcgtgg gatgtggata aaagacccga
      181 ccctgagatt gtttcataaa ctggcgcatg aacgattctt cgccaaaata gcccatctcc
      241 cagtcagacg ttaaaaggaa cgtgttgatg acaatgttgt cctgcgtgca gttgcgcact
      301 tcgcgcagtg cgaattcaaa atccgcaggt gaaggcgggt aatttacatg cagactgctg
      361 ccttcaaaat gagccgtagg caccccatct gtgatcaaga tgatttgctt gttctttgtc
      421 tctccgcttc cgagtagagt acgcgataac tgcagccccc gttgcaggtt cgtaaaatac
      481 ttaggaattc tatctttacc cttttctgtt tgagccgcca tgttgtatct caaacgaatg
      541 tgcggatcgt acatggtcac tggatgtggc tggagactcg gaacttttgc tggcggaatt
      601 acacttgcta gagtcccaaa accaacgatc gtcaagcgat catctttgaa atcctgacga
      661 atcaaagcat cgagagccag cacaacgcgt ttcgcattgt aaaaacgatc cgaccgcatc
      721 atgcttccac tcatatcaat cagaactaca gtggagcttc tagcagatcc tcgcgcttca
      781 aatacttcca tatcagagag gtccggcttg cttgactttc gcctaacgta gccgttgacg
      841 atcgaagaag acaagtccag attggatact ggatctccaa aatcaaatgg cctggttttg
      901 ctcgaaacgt tttcgctttc tcctgtttca tccgcagaat gcatcccgcc ggatgtatcc
      961 gcttttagtt tactgaagat ttcctcgagc gcacgccttc caatctttcg cactgattca
     1021 ggcgaaagac gcatctcccc tgtttctgga tccggaacaa tccttccctg tttttctaaa
     1081 agctctctga gcttttcaaa gatttgttct ctcttttcta gaaactcctc aaactgttcg
     1141 ggcccgagat attgagcaag cttctccagg ttaaaattga agaggtcacc actttcttct
     1201 gcttctttca aagcctgcat tagcttttct aagtcatcca gatgcttgag tatgtcaatg
     1261 gcttcgtcgc gcgaaagggg agtggagcct gtgaacttat acttcttttc tcctgccacg
     1321 agatcatcca ggtcttccat gtcctggatg acggatgcta tgtcgtccat agaaaccgga
     1381 cgcttttttt cctgcatcac ttcattcttg atgcgataca attcatcaat ggacaggttg
     1441 cgaatcgcat cttcgattct atccttccat tctttgctct tcgccgtatt tagcagatcg
     1501 ccagaatctt tttttaaatc aaattctgtg tcctttcgcg ctggcttaat gtcaaattga
     1561 ccgagcacat catcgatctt ctgttgcaat tgctgttgga gactatggac cagatcgtcc
     1621 ataccgcctt ccttcagaaa catattgacc ggcaacccac gatcgatgag cattctgaga
     1681 gcttcgtcca attccaggtc gtatcgcatg atcatgtccg aaaggacatt catcaaacga
     1741 tccatgtccc aggggcggtc acgttcgggt tcgtatgggc catacctgta agtgatcata
     1801 caaatttgct tccaattcca gtcggtctct taccagatgc catcgtcagt cgtcagcaac
     1861 aaataacagg atagcacccg gccggcagaa tggaacttac ttttctgacc tacaatatcc
     1921 atcgggccat tggattcgat cgcaaataca ggctcgaaag gatcatagaa acctgccgcg
     1981 acgtggatgc ggacattgtc gccctccagg aagtggacca atttgcccct cgcagcaaag
     2041 gcgaagacct ggcgcatctg attgcgcaaa atctagacat gcactatgag ctcggactga
     2101 atgtgaggct tcagaaaggc cactacggca acgccacttt ttcaagatat cctattgaac
     2161 aagcccacaa cttcaacata acctggggca ttaagaagaa acgaggttgt ctaatcacaa
     2221 gcattggctt gcctcatgcc aaaatcgcta tcttcaattt tcacatgggc ctggcgcgca
     2281 tggagcaggt tagacagaaa aagaaaatcg ttcattcaga ccatatggag cgcctcgctc
     2341 gagttcccat ggttctcctt ggtgatacaa acgatcggac taccagactt tctcactcat
     2401 ttcttgaatc tggatttcgc gatacatgcc caggaaaggg tcacaatact tttcccgctt
     2461 atgctccaat tctcagactc gatagaatct ttgtcactca acaatgggaa gtagtggagc
     2521 acagagtcgt gcgaaacaaa ctcactcgaa tcgcttccga tcatctaccc gtagtggcac
     2581 gcttaagact taagtcttac tgatctgatt atacaaatcc gttcagttgc ggatccttct
     2641 caagtacgtt cgagatcatc tcttgaagac cggttagata atgcatgtat ggttcaggaa
     2701 gcgaatcacc ttttcgattg gttacttgct ctcttagttt tcgcagagtg gaatgcacct
     2761 ctccgagttt ctgcaaatgc tgttcatgat tgtctttgcg aaacatcgca cgaatgttat
     2821 acggatagat atcacgtatc aattgcccaa caaagagctt ctctttctcc ggacgaatga
     2881 tcactgtttc ttcgcgagca gacgccttat actttgcgta cgtaatcgtg tatccaaaca
     2941 gttccttttc aatatgagcc acagccggat ctcccggatc atgtctgcga tatgattccc
     3001 atttttcctg aatggatttg ctgattggat ctacaggagc agcctgcgtc tttaattctg
     3061 aaatttcttt tacgtcaaaa tgacttgagt acgcgctgat gatgtctggc gattcacgcg
     3121 caagcatgtc tcgatcctgt tgcgtgcaaa gaaaaagtgc cgtctctccg tctttcacaa
     3181 tgtgatgact gtctaccggg cgacgcgccg ggaatcgtcg catggtttga taatccataa
     3241 cggaaaacgg acttcctgga tccctgcgat ttgagagaaa cacatgcaac attccgtgat
     3301 cgcgcacaat agttaccagt acgtcccccc gctcaggatg caaaagcgat tggagagtaa
     3361 tctcttcatc ctttagaagc gcgcttccat gcgcagtgat gacctgtctg gctttgattc
     3421 ccgcgatcag gttcagtaga actttacgaa ccttttcgat ttcctctggt ttcaggtgcg
     3481 atagatcaaa ctgagtggac attcggttgc tccgccagtg tttccggatg aacccgggtt
     3541 aagttaactc caccgctcac atagcagtgg cggcatccgt aaatgtgtgc ctgtcgcttt
     3601 tgatttggac cgagatcagg aaagattgca gcgattagat attcgtcctg actgagaggt
     3661 gttccgcaga cgggacatat ccgaggacgt tccgccgctt tcatacgcag aatacgtgcc
     3721 gtatccagag tcacaggttt ctcaatattg gcgggcgact ttttcgcgcc agcagacgtg
     3781 ggcagattca acgcgttcag gaagacgatg agtgcaaggg ccagtgccgc aatcgttaca
     3841 aatgttagca tgattctacc actatagact gtttcggtcc cagaggtctc tatctggagc
     3901 ctgagcccgt ccagaacagt tctttcaccc acttctggat tggacgatca taatcctgcg
     3961 gatgctgggg attagattct ggactttttt caaaatggtc ttgctttgaa atgaaaattc
     4021 ctgcatatag acgccccccg cgacactcac atacaaggct tcattctcta ttttttcagg
     4081 cctggtccgg gcagacagat ttcctgcaat ttcgtcccac gatactccca gaagcatcaa
     4141 ttcacgcgtc ccttcatcaa acggaagaga tcgcacgaat gcgttcttga gttctcgcaa
     4201 ttgcgcagac tggggcacac caggagcctg ctcttcatcc aacggattgt tcaaccctgc
     4261 catccccaag aatatgaatt acggaactct gactgaccag agatttaagg tcctcatcga
     4321 tcccgtctag atccggtgta gtaaaaatcg cctggccact ttcgcgcagg agtttcacaa
     4381 aggccgatct gcgcattgta tcaagttctc gaattacatc atcaatgaga agcaaaggtg
     4441 ttatctttag tttttgcttc aaatgatgaa actgtgcgat cctgagtgca agtgccgccg
     4501 ttctcttctg tccctgactt ccaaattgca tgatatcacg accatctttc aagaaaacga
     4561 gcgagtctct gtgcggacca cttgtcgtgt atcccagtga tagatcgcga accacgttct
     4621 tatcatagct ttctttgaga tcatctctgt gcgaaaactg aagctcaaga ttcatcgcgt
     4681 ctctgtttcc ggatatttgt tccagagcgt cgcgaaacgg ttccttgaat gaaagaagaa
     4741 aattttccct tctgctccag agctgagatg caaattgaac cagtctggca ttccagatct
     4801 gcaaatctgc gatatttgcc tgtctttgtc tgatcttctt caatagggca tttctttgtc
     4861 tgagtgcttt ttgatatccg atcaagtttt ggagatactc tggttccatg ctagaaatcg
     4921 cagcatccaa aaactttctt cgatctgcag gtgaaccttc aatgatttgt aaatctgaag
     4981 gagaaaagac aacacagatg aattctccta ctatcgatga tctcgaagaa gccgctttgt
     5041 cgttaaattt tattttacgc cgtaaatcac ctgtggaaac gtccgttgca atttccattt
     5101 ctcctttctg gcctgctcta gaatacttcg cttttatata gtatccattg gatccgtatc
     5161 ggagcaaatc gtgatcagaa gctcctcgaa aagattttcc gtgcgaaagc attccaatgg
     5221 cttcgagcaa attggttttc ccggccgcat ttcttccaag aaggaaggtt aagcgagacg
     5281 ggaataaaat agatagtgat tcgtagtttc taaaattctt cagtaagagt tgttcaattc
     5341 gcacaagcga tgatcaaatc ttcattggca taaccacaga taagaaatct ggatccgccg
     5401 gatctcccac tgtgcacggc gaactgggag aagaaaaccc aagaatcaca tcttcacccg
     5461 gaattacttt tagaacatcc attagataaa cggaattaaa tccaatcacc atttccggac
     5521 cggagtattc gcattcgaga ctgctttcac ttccccccag atcaggtgtg ctggcttgaa
     5581 aaccaacggt cccttcagaa aaaataaatc taacctggcg agcaggctct atggcaactg
     5641 acgccgcctg tttgattgct ttttccaggt caatgcgatt gatcctgatt ttgtaatcta
     5701 actttcgtgg aatgacttgt tcgtaatcag gaaatgctcc atcaattagc tttgttacaa
     5761 gatctactct accgattcta aaatgaacac gtttgtcttt gtcatcgtat gcgatcattg
     5821 cttcttcgcc tgcatccagg aggcggacaa gctctttgat tgcttttgcc ggaatgatca
     5881 cgccatcttt gaaaggaagt gcagatggaa agtctctgga gattttcgag agtcttcttc
     5941 cgtctgtcgc aacaactata ttggaatttt tctgagactt tagaaacaaa ccattgaaca
     6001 catatctgct atcatcctgc gccatcgaat agtatgtttt caaaatcatt tcgcgaaaaa
     6061 ctgcaggcga aaaagaaata agatcttccg aagaaggtga aggtgttgga ggataatctg
     6121 aggtaggaga agttaccaga ttcactctcg gtttggttct tgcttttgga gaagaaatga
     6181 tgagtttgtc atcatccaaa gagatctcaa tggtctctcc atccatagct ccaatgactt
     6241 gtgttagttt tttcgcgggg actgtagttc cgcctttctt atccagtgta ataggaagcg
     6301 ttgtttttag agcaatctcc agatcagtgg tcgtgaacac tgcttcgccc ttgtcagctt
     6361 cgatcaaaac gtttgaatat acagatctaa tttcgcgtga tgggatgata ccgtctacgg
     6421 tggtaattgc tttcaggaac tgctgtctat ctactgtgaa cttcatggtg cccttcaact
     6481 atataatgat tatgtgtttg tacttgtact ttgttgtacc tgtgaataag tcagtactgc
     6541 cgtaaatctc gcttcccaag cgacggaacg atttcctgtc cgtaaaggat tatgtcaaaa
     6601 taaaaagaca actgtcagaa acactccaaa aagaaattca ctgtgcgaaa acggacctga
     6661 ttttgaacta aattctttgc tttctgacga caggtttgtc aattgttcac agtgtactca
     6721 caatggcctt gagggcacct gccagtgcgt cgtcgttttc tagagcaatc tgaatcttct
     6781 tttctgcgtg cactaccgtt gtgtggtcct tcttcttgaa aaaccgtgca attgctgatt
     6841 tgctcattcc accctgggtg agagccaggt acatggccac atgtctgggc cacgtatgct
     6901 ccgtttttct agaagaactc agaatgtcct gtttagaaac gtgatagtga cgagtgactg
     6961 cctcaagaat ggcgtgaatg gacgggattg ccggtcttcg cagggttcct tctatgacag
     7021 aatcgatatt ggaggtagag agatgtttgc tttccctgga ctgaagttcg attttaccga
     7081 agatggattt gatttcacga atgtctgttg cggatttgca aattctttgg atcgtagaat
     7141 ggtctagaat gttgggattc acctccgcca ttttttgaca gaacgctctg cgcaagtcag
     7201 catccacaga ttccatttga acaacaatgc ccgaaagaat tctgcttcga agtctgccgg
     7261 aagggaaaac agaacctggc tctgtggaac tggaacatac gatttgtttg cctcggtcga
     7321 gaaaagcagt gatcaggaag tcaatttctt ctagagtgga cttgccgctc ccgatcaagt
     7381 ggatgtcatc gaaaatgacg caatcgaaag acatcataga ttctttgaaa tccagggaag
     7441 acctcgaagc gatagcgcga agatgcccca gcttaaaagc gatccccgaa agatatgcaa
     7501 tgcgtgcgcc tggctttgct tcgattacgc ttctcgcata actgtgtagt agatgagtct
     7561 taccggaatt ggcgggcccg gacaaaaaga ttggattgtg tctgcccggt tggcgacacg
     7621 cggctctaat gcattgaagc gcgtttgaat tgcattttga ttctagaaaa gtttcgaagc
     7681 tgtattcggg atgaatccat tccggcagtg aatgggatag ttggaattca acgcgaaccg
     7741 gccgtcctaa aatgctggcc atcttcactt caatcgggcg agtgtattct cgcaaatgtg
     7801 acagaattcc attatcgggc acattgagaa tcagtcggga tgaatcagta ctggttccgg
     7861 gcttcgcgga atatagccat ttcaggcttg aattctggga ggattcgcct gcgaagtctt
     7921 tgaggacctg attccacgcg tctttgatca tgatggacgg cggaatgaaa tttgaggcgt
     7981 agaagtcagc aagccaaaaa tgtaatctct tcaaaaacag ttgaccctga tgcctctttc
     8041 gaggtttctt gatcaaacag tctgagattg atcatatttc tcatcttcag ccagactcag
     8101 gaaacttaaa ctaacatcga tcgcaggatc atcacgattt ccctatgaaa agaacatttc
     8161 aaccaagcaa taagcgtaaa aacaaaactc atggctttcg ttctcgtatg gctacggcca
     8221 acggacgcaa agttctctca aacagaagaa aaaaagggcg caagcgcctg actgtttctg
     8281 atcaaacaca caggaaataa tttctgtccg gcctgcgcgc ccgtaccctc cgcggagaga
     8341 gccagttttc tgcgcttttc cgggaaggca gaaagatgca ttccagagga atcatgttga
     8401 tcgtatcgtc cggcaatcct ggcgggatag ctgtgtgttc ttcacgtatg aaaagagctg
     8461 tggatagaaa tagaagaaaa agaattatcc gctccgcact tcagcctctg gttcccgcaa
     8521 tcaaacccgg gtgttctgtt gcagtatttg ccgatcttga attcgaaaaa ctttcccttc
     8581 cggaaagaac agcgactctt cgtgatgcgt ttgttcgtgc aaggctgctt gccccatgaa
     8641 tgcgaacact ctggccatcg cagggattcg cgtttatcag aatactattt ctaaactcat
     8701 tcctggtcgt tgtcgctttt atccaagttg ttcgagttac gcagtagaat gcttcgaacg
     8761 tttttcattc gggcatgcgt ttatcaaatc aggctatcgt attctcaggt gcaatccctt
     8821 caatgaggga tacttcgatc cagttattcc agacgaaccc caaaaccagg cgtaaacaaa
     8881 atggaaaata aagaagaaca aaaacccgga ctgggttctt ttctgccagt actgctgatc
     8941 ggtattgcgg tcctactgtt ctttcaattc agcggaagcg gatctcatcc cccggtgact
     9001 caggagcacg aaggcacggg tgcgaaatta accgattttg aatttcagtc gggtcccggg
     9061 gaaattgttt ctgtagatac tggcaacttc atcgtgaagt tgaacagcca gggcggacga
     9121 attgataagt tctacgtaaa gaaaagcaaa gaactcaatg ttcctgattc cgtactgaaa
     9181 acggaaactc cggaagcgca atcctaccag gccttcgagg gaagccgggg gaacggattc
     9241 gactttcaat ttcatggata ctactatgga gacggagcct cgcagctcgg tgatccggcc
     9301 ctgaacaaag cgctcttcaa gaaagaagga ccttttcagg acaaagatgg cgtcgtagaa
     9361 gtgcgttttc gtcttcctgt tcgacttcgc ggacaaaatc tcgaaattgt taagatctat
     9421 cgctttttga aaggagagca tttctttcgt caaatcgctg taatacgaaa cctggacaaa
     9481 aaagaattcg tctggggcgg tccaatgttc tttcgcccct ttggggatgt agggccctct
     9541 acagaaatgg ccggagggat caacccggct ctgcacggca ggttttacta ctacaatgga
     9601 gaattcaaga gcagaatgaa tactccaccg accaatagca ttcttgctcc cattggttgc
     9661 ggtgaaaatc taaaagataa accttatagt gtttatgcgg aacatcccaa ttcacttcat
     9721 tttgttggcg cacacagtaa gtatttcttc gcatacgcga agtttctgga tacaaacaat
     9781 cctcagagcc aggtggatgg cgtagccatt agaaacgaaa tcgatccgga cggcaagcgc
     9841 gcattcacta cgattctcaa cgactttagg ctcgcgccca acaccgggga gcttgaattg
     9901 cagcctgcag gggcgcagac gacgggcggt catgaataca tcaaagggat ccagcagaga
     9961 aatgatgcag tcattctgga tcaggaaatc tattacggcg tgcgaacaga cgaaagtcat
    10021 gcttttgcaa acgaagcgct ggccaggact gaattcggat cgtctgacac ggacaaagaa
    10081 gcacgcggag tcatttacac ttccagtttt cttgcgctct tctcaaaagt ccgcgatgca
    10141 atcgtatggg tgatgagatt ccttcacgta tatctgggca actatggatg ggtcatcatt
    10201 gtcatcgctg tttccttcaa gttgattacg tttccgctga atcagatgca ggccaagagc
    10261 atgaagcgaa tgagcgccct aaagcctgag ctggaaaaaa tccaggagaa gtatgcggac
    10321 aatccggcgg aaaaacagaa gagaaccatg gaggtttaca agaagcacaa cgtaaatcct
    10381 gccaaaggct gcgtgccgat tgtgatccag attccggttt tcattgctct ctatagcgcc
    10441 ttttcagagt ccattgaact ctggcggagt ccgttcatcc tgtggatgac agatctatcc
    10501 gcgcctgata caatatacac catcaaagac tttctgatcc taaaggattt tcatatcaat
    10561 gttctgccgc tgtttatggt tgcctctcag ttgttacagc aaaggctcac aactgtgatc
    10621 acggatccgc agcagaagat catgatgtat atgatgcctg tgattatgat tttctttttc
    10681 tggacaatgc ccagtggcgt gacgctttac tggactgtcc agaatgtgct cgccatcgtc
    10741 tggcaactgg tgaccaaccg gatgtccgat gacgggaaga ccgcctgaac catttttgga
    10801 gtaagagtat gaattacatt tacgaggcag aaggcaagac gcgcgctgaa gcagaacaga
    10861 tcgccttgag cgcgcttgga ttgaaagaag aagatgttac gtttgaagca gtttccgccg
    10921 gaaagagttt cctgggaatt gtaaacagga aaccaaccgt tgttcgtttg ttcgcgaaga
    10981 acgttcctat cgaaacagtc atcaaaggca ccgtgatcac actgattagg aagatgggtc
    11041 tgacagttga gaatgtggga gtccatattg aagaagaaag cttcgtggtt tccattgatt
    11101 ctgaagattc tgcgatcttg attggaaagc agggaagaac tctcgattcg ctgcagtttc
    11161 tcaccaattg catggtggat tcacgcatcc ggaacaatcg tcgtgtggtt ctggacgtag
    11221 cagaataccg cgagcgtcgc aaacaaagat tgaccaagct cgcaaaggca gtggccgatc
    11281 gagtggcgcg cacttcccag agcgtattgc tagaatggat gaatccgtat gaacgtagaa
    11341 tcgttcatct tgcgctcgaa ccagacgaga gagtaaccac tcgatcagat ggaaacggag
    11401 tatacaagag agtgcgagtt cttcctcaga aaggtagacc cggttctgga cctcaggatc
    11461 gtcaggatcg ccctgatcgt caggacagag aaagaaactt caatcgagct cctcgcgaag
    11521 aagaagagga agatcaggat tttaatagaa aggacgcttt cgacgaccaa tgaccattgc
    11581 ggcgatcgca acgtacccgg gccacgcggc cctgggtacg attcgcgtgt ccggtccggg
    11641 tactctagat attatttcca ggttcgttgt tgcaccggac ctgaagccta ttccgttttc
    11701 taatcagccg cgccattcat ttttcgcaaa gttcatggac gcaaatgtgc tcgtggacga
    11761 ggtcgtagcc gtttacttca aaggccccca ttcctacacc ggagaagact cagccgagat
    11821 cacctttcac ggaaatccca ttttactgaa gagcgcctta cgactgttag tcgaatcggg
    11881 gattcggctt gctgagccag gagaattcac ttcgcgtgcg tttcgcaatg gtcgtctgga
    11941 tttgacgcgc gcagaatctg tgaagaggtt gattgaagca aaaagtgagt atgagctttc
    12001 ggccgcgcgc gctatctatg gaggagagct ttctcgcagg atgtccattc tcaggtcggc
    12061 catgattggc ctgaaggcgg agacggaagc ggaagttgat ttttccaccg aagatctcac
    12121 ttttcaatcg cgcgaagaaa ggcgcgcacg agttatggga ctgatcgcag agctggattc
    12181 gctccttgcg cgcggaagtg caacaggaag gattcgagaa ggaatttcta ttgcgattgt
    12241 aggtgtgccg aatgctggaa agtcttcgct actcaatcga cttctcggct acgatcgagc
    12301 cattgttaca gaagtggcag gtaccactcg agatactctt tcggaagagt tgcagattgc
    12361 tggaattcct gtgcgtctcg tggacaccgc tggaattcgt gagaccaatg atattgtaga
    12421 aagagaagga gtgcgcagaa gccagcgtga gctcgaagaa agcactcttg tgattcacgt
    12481 aatagacggt tcacgcgaat cctacgcttc gattgtagaa tcggaccgcg acaacgtaat
    12541 ccatgtaatc aacaaagcag atcgtcttca tccgggcgca aatccaggga gcgcgattca
    12601 ggtttcctgt cttactggtg caggaatgga tcatgtagaa gaggcgattc gggatagagt
    12661 tcttgaaaga ccggaaagtc gtgatcctat attgctcgaa gaaagacatc gctttcatct
    12721 actggcgatg aaggcgacgc tgacaaagat catggaactc tggcagcaga atgcgcctga
    12781 cgaaatcaca gcccttgaga tcgatgacgt gatcgatcat attggtagca tcacaggacg
    12841 gatttcaaac gaggaaattc tgggacgcat tttttcgacg ttttgtgtcg ggaaatgacg
    12901 gcgaaaatcc gcttgcgtac cgtatagccc gaagccactt agaatacagg agactcatat
    12961 gcttaagatt ggaatcaacg gatttggccg aattggccga ctggctttcc gcacagctct
    13021 ggaaagacag gatatcgaaa tcgtgggaat caatgacctg ctcgacgcag actacatggc
    13081 ctacatgctc aaatacgatt caactcatcg cgctttcaaa ggaactgtgg aagcaaaaga
    13141 cggacaactc attgtaaatg gaaagaaagt tcgcgtcact gctgaaaagg atcctgcgaa
    13201 tttgaaatgg ggagatattg gtgctgaagt tgtagttgaa tccaccggtc tctttaccac
    13261 gattgaaaag gcacaggctc acatcaaagc cggcgcgaag agagtggtta tctctgcccc
    13321 ttctgcggac gcacccatgt ttgtcatggg cgtgaatcac aaaacttaca aagcagacat
    13381 gaccattgtg tccaacgcga gctgcacaac caactgcctg gctcctcttg cgaaagtcgt
    13441 gaatgatcgt tggggaattc tagaaggttt gatgaccaca gtccacgcag tcacagctac
    13501 gcagaaaacc gtagacggtc cttctcacaa agactggaga ggtggtcgtg gcgcgtatca
    13561 gaatatcatt ccttcttcca ctggcgcagc caaagcagtt ggaaaagtga ttccagatct
    13621 aaacggaaaa ttgacaggca tgtcctttcg cgttccggta gcagacgttt ctgtagtgga
    13681 cctgacttgt cgcctttcca agcctgcgaa gtacgatgat atcaagaaag caatgaagga
    13741 agcttccgaa ggggaattga agggaattct tggttacacg gaagacgaag tagtttctca
    13801 ggattttctc ggggatccac gcaccagtat tttcgatgcg ggcgcaggaa ttcagctgag
    13861 cgatactttc gtgaaactcg tttcctggta tgacaacgaa tggggctact caaacaaact
    13921 tgtggacctc atccagcaca tcagtacagt aaaatgattt aagtaaaagc gcgagggcaa
    13981 atgcgatgaa caagcttacg gtaaaggatc taaatgttgc cggcaaaaag gttctggttc
    14041 gagtcgattt caatgtgcct ctcaaaaacg gagtagttga aaacgacaaa cgccttcgcg
    14101 caagtgtgcc caccgttcag tacctcattt ccaacggagc ccgggtaatc ctcatgagtc
    14161 acctgggacg tccaaaagga aagcctgtag actcgatgcg tctgggcccg gtggcggacg
    14221 ctttcggcaa attacttggt aagcctgtaa aaagcattgg aacttgcgtg ggcgatgaag
    14281 ctcaagcggc ggcctctgcc ttgaaagatg gagaagtgct gctgttagaa aacctgcgct
    14341 ttcatccaga agaagaagga aatgatcctg gctttgcaaa gcagctagcg tctctcgggg
    14401 atgtgtacgt aaacgatgcg tttggaacag cccatcgcgc gcacgcttct acagaaggca
    14461 tcactcacca tgtcaaacaa tctgccagcg gattcttgct acagaaagaa ctggattatc
    14521 ttggtggcgc ggtaaataat cctaagcgtc cgttcgttgc aatacttggt ggttctaaag
    14581 tatctggaaa gatagacgtg atagaagcgc ttctgcccaa agtggatttt cttttgattg
    14641 gcggaggaat ggcctatacc ttttacaagg cccagggtct tgaaatcgga acttccttat
    14701 gcgaagaaga tagaatcgat atggccaaaa cgcttcttgc caaggccggg acaaaacttc
    14761 tattgccggt ggactgtgtg atttcagacg catttgattt tgatgcgcgc aaagtgggaa
    14821 ccctgaagac tgttgctgca aatgggatcc caaaaggatg gagtggtctg gacgcaggcc
    14881 ccgaaaccat ttctctcttt aaagacaaag tactttctgc cggaactgtt ctctggaatg
    14941 gtccccttgg tgtttttgaa attgcagaaa cagcggtcgg aacttttaaa attgcagaag
    15001 ctctggctga ggcaacgaag aagggtgcca ttacgatcat cggtggtgga gactccgctg
    15061 cagcgattga aaaggccgga ttggaagaaa gtgtgagtca tgtttctaca ggtggcggcg
    15121 cttctctcga gttcctggaa ggtaaagacc tgcccggtgt gagtgccctg accaatcgtt
    15181 gagcgttctt cgcggactca gacgcctcaa tcggtatgag gccaggttgt ttcgaattgc
    15241 acgcaaagca ggaaagctcg cgcaaataga gcagctaggc tattctgcca gaagctcgga
    15301 cggatttcga tttcctatat attctttaac aattggaaaa ccggctgccc tggaaagatc
    15361 tccggttgga ctggttgcgg gcgttcacgg tctggaaacg atcgggattc gcgtccttct
    15421 tgattttcta gaaagcatat ttgatgagcc caatcgcgat gtatcgcagg ctctgctcga
    15481 tggggatata gggattgtta gcattcccat cttaaatcct ggtggagttg ctttgaaaag
    15541 tcgagcaaat cctgcgggcg tggacctgat gcgaaattcg ggaaaagacg gggacacagc
    15601 gctcccattc ttcggcggtc atcgcatgag ttcgaagttg ccatactttc gtggcaaaag
    15661 tcttcagcca gaatctcgcg cactctttcg tttttgtttg gagcgttttt ctcaggcgcg
    15721 taaaggaacc attctacctg tgctggatgt ccattctgga tttgggatga aggatagcgt
    15781 gtggtggccc tatgctggaa agaagacacc gtctgcggat cactatctgt tcgaaagact
    15841 gtcgaggatt ttacacacag aaggcgacgg aattacgtac gagccgcaga gccaggctta
    15901 caccgtacac ggcgatctct gggatcgcct gtacgatgac tatcgtcgta gcaagaaacc
    15961 cgccaaactt ctgccattta ctcttgaaat tggaacctgg tcagacgtta agcggaatcc
    16021 ggggcggctt ctcagtaaaa gaggattgtt caatccacca cctgaacaaa aggctctgat
    16081 gatccagaga tatcgtcctt tcctgagtaa gttcgtcctt ctttccttgc tctatggcga
    16141 atgactattt caggaatttg tttccgtact tattggtgat cgactctgca tcaaagtaga
    16201 tgaatagatc tgtcgttcca aattcacggt ccagcgcggg ttcgccgcca atgcgcgcac
    16261 caattctcat atagcctttg atcaaaggtg gcaagcgttt cgtgatg
//
LOCUS       DAORFF010000010        16178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1482_length_16178_cov_3.264095, whole genome shotgun sequence.
ACCESSION   DAORFF010000010 DAORFF010000000
VERSION     DAORFF010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 16178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16178
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1482_length_16178_cov_3.264095"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PKK76_00870"
     CDS             complement(<1..760)
                     /locus_tag="PKK76_00870"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNL67042.1"
                     /translation="MAFFDPPSYLRPAWIQTLLPAIPFGVKRKHPVLASAEDIILQVD
                     GIRIHAALSRGTRRAIVILIHGWEGHIEAPYMLRTSQALSDHGFYVARLHLRDHGATH
                     HLNEELFNGSMLDEHFEAVRQIAKMFPGFSVYLGGFSLGGNFALRIAARNSRVKTPIV
                     NLKAVAAISPPLDPHRSTASMDEHRMLGRYLLWQWWRSLRTKEKLFPEKYNFSHLRKE
                     RSILNLTERLILAHSPFESLEEYFGTYTLGSEFFS"
     gene            866..2137
                     /locus_tag="PKK76_00875"
     CDS             866..2137
                     /locus_tag="PKK76_00875"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL67043.1"
                     /translation="MRPDILSHSNLLDEEIHSTFKTGSLYSAVMGLSSACLLSAMQWT
                     GMIGSIEVPILWTVFGGLYSLGVFLIARARKLKGLWIPFALIGFTTLPTSIYVISHWV
                     LPSGAATYITGPPSYLYFCMIAVSGFAFDPRISTIAGIVSALQYVGAFLIDRDHLRIT
                     SQDPLMLQDLTSTSIYLFKGMMMVFTGLVVAVLATTAKKLIQGIVAEEKEKFSIKRVL
                     GEYVSPEVADKIVASQKIAEKKQVAILFSDIRGFTSFSEHNEPDAIVERLNEYFSAMV
                     SAIEKNGGTVDKFIGDAVMSVFGGVLPLENPSEAALNAARQMRASLKELQRGWASTGQ
                     QVFDNGIGIHFGEVLQGTLGSEGRKDFTVIGDAVNTASRLEGLCKETNTGILISAQAV
                     ERIPESSRVGLRSLGKFQVKGKIKEIEIYGE"
     gene            2157..2801
                     /locus_tag="PKK76_00880"
     CDS             2157..2801
                     /locus_tag="PKK76_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNL67044.1"
                     /translation="MSFLKDLKKNNDRAWFEKHRDRFEAAHEEFSQTAQGLIEQIARL
                     DPGIRPVSLKDSIFRIYRDVRFSRDKSPYKPHFSFFAARGGRKSKYAGYYVHLEPGSC
                     FIGGGIYHPEPENLMKIRTAIAARGEELRRIVQAVPFRKYYKGLEGDRLKNPPRQYAK
                     DHPLAEFLCFKDFIASAKLKDTEVTGDLAGACIMRFRALLPFNRWLSRAVDQDS"
     gene            complement(2761..3177)
                     /locus_tag="PKK76_00885"
     CDS             complement(2761..3177)
                     /locus_tag="PKK76_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL67045.1"
                     /translation="MAKKRVLDVGQCRPDHAAIRYVMESLGAEVDAAYLPADALEAMK
                     RERYDLVVINRKLDSDYSDGTDLIRMMKADRSTAEIPVMLISNYPDAQEEAVSLGALP
                     GFGKLEMMKDSVHARIRAALESSVKNPGPRRAKATD"
     gene            3258..4352
                     /gene="ychF"
                     /locus_tag="PKK76_00890"
     CDS             3258..4352
                     /gene="ychF"
                     /locus_tag="PKK76_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNL67046.1"
                     /translation="MSLQCGIVGLPNVGKSTIFNAITRAGAQSANYPFCTIEPNVGRV
                     DVPDTRLTKLSDIVKPKNVVPAAMEFVDIAGLVEGASKGEGLGNQFLSHIRETEAVAH
                     VVRCFTDDNIIHVRGKVSPADDIRIINLELILADLDSLEKQIQKIEKKAKSGDKVEKA
                     RFDLAAKIRDHLNEEKPLRTMSLSDDEKAIAKTFALITARPMLYVMNMDEASASSGNE
                     YTAIVEKIAAEEGAPTVRLCGRTEEEIAALSPEEAQEYLSSLGLEESGLARMIRASYT
                     LLNLLTYFTAGEVEVRAWTVTKGATAPEAAGVIHSDIQRGFIRAEVMSCDDIFKLGSQ
                     QAVKDAGKMRLEGKDYIVQDGDVCYFRFNV"
     gene            4380..4904
                     /locus_tag="PKK76_00895"
     CDS             4380..4904
                     /locus_tag="PKK76_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNL67047.1"
                     /translation="MIYRFDQHTPQIAPDVFIAPDATVIGQVRIGAGSGVWFGSLVRG
                     DVHSIDIGEHTNIQDHCVLHVTGGKFALRIGNHCTLGHRVTLHGCTLHDHAFMGIGST
                     VLDGCVIEEFGFLAAGSLLPPGKTIPSRMLAMGSPAKVVREITAEEEEMMRRTAVKYS
                     ELARVYRSANFGPV"
     gene            4882..5328
                     /locus_tag="PKK76_00900"
     CDS             4882..5328
                     /locus_tag="PKK76_00900"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNL67048.1"
                     /translation="MQTLDPSDIQKRSDRTELRIFQGGAVVVFLAGLFLPLTRFPSIC
                     IFLYITGLPCPGCGLTRSIHLTLNGKILQALQFHPFGLPALAWLCFLILSIFIPSLSR
                     FYVKHIKQIHRLYATGALLMLAFGVVRLIGLLMFGGLSWVAPSVLR"
     gene            5370..5792
                     /gene="ndk"
                     /locus_tag="PKK76_00905"
     CDS             5370..5792
                     /gene="ndk"
                     /locus_tag="PKK76_00905"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNL67049.1"
                     /translation="MEQTLIILKPDAVRNKHVGNIIARIEKEGFNIVALKKITLKDKD
                     AKKFYAVHKERPFYKDLCKFMTTGPVVVAVLEAPGAVEKWRKLIGATDPAQAEANTIR
                     KLYAESKEANAVHGSDSEKNAKGEIKFFFKAAELKGKL"
     gene            5793..6695
                     /locus_tag="PKK76_00910"
     CDS             5793..6695
                     /locus_tag="PKK76_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNL67050.1"
                     /translation="MSDSEISLLREEFEQFLGEAGEIFSNGTVADLSAPVLYSLSAPG
                     KRLRPVLCMMFGGYQNKTQSRGLLFAAAALECIHTYSLIHDDLPAMDNDDLRRGIPTC
                     HKKFSEWAAILAGDALNTFAFELLAEAYEPVLLPEAVKTLARKGGRVGMIAGQALDLK
                     NERGSSSQESPSNANLQDTLQNIHLRKTAALIQASCVLGSLYAKTETESASGFGETLG
                     LLFQVSDDLLDARGSNDAGKATLKDAKAGKLTYPFLYGMEESMRICADLETRALSYLA
                     RAPISETRRHALAELTRSLKDRTR"
     gene            6692..7264
                     /locus_tag="PKK76_00915"
     CDS             6692..7264
                     /locus_tag="PKK76_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNL67051.1"
                     /translation="MKGRGARKLELALEQIHLPIQDSYCIDLGASHGGFVRTLLENGA
                     ARVSAVDVAYGILDYSLRVDPRVSAIERSNVRNIEPSWFEDLPSSGKIIVTCDVSFMS
                     IRTVLASLADFLHGTGLAMEGLFLVKPQFEDSKSTVKGILKDPIRREEIISEIRSFCV
                     TKGFEVKGRMDLPEISGKNLEVFLHLSYEK"
     gene            complement(7266..8537)
                     /locus_tag="PKK76_00920"
     CDS             complement(7266..8537)
                     /locus_tag="PKK76_00920"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNL67052.1"
                     /translation="MSAALVVGTQWGDEGKAKVIDYLSSKIDFIVRYQGGANAGHTVV
                     VDGETYIFHLIPSGILYPNAVCVIGNGVVLDPEAFRDEVRALARRNIEAKERVIISDA
                     CHLVLPLHRIIDAHRENAAGEKKIGTTKRGIGICYGDKTMRIGLRAGDVLHADRLRDR
                     LGHILEIKNYELKNLYRLPEVEFNALYEDLLAFGNEIRGMVKNTSLLLNENLAKGKTV
                     LLEGAQGTGLDIDFGTYPYVTSSNTTTGGAIAGSGIDFRFLKTVTGISKAYVSRVGEG
                     PFPTELFGAEVEKLRELGHEYGATTGRPRRCGWFDVELVRHAARVNGLTEIALTKIDV
                     LSAYDTIKIGVGYKRGGEKLPAFPTDGYLEGVEVVYEEMPGWKKEIGHVLKYSDLPPE
                     CCKYIRRLEELCEVPIKLVSVGPGREHTIPL"
     gene            complement(8576..9511)
                     /locus_tag="PKK76_00925"
     CDS             complement(8576..9511)
                     /locus_tag="PKK76_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNL67053.1"
                     /translation="MKNPELKERFKSWIPHGMEFIGPDQTERILAGCARLREAFAALG
                     AREVIPPLLDFAPTFEMARQSEMASFQTRDMAGELLAFRSDLTVQIIKAAASGVFGQP
                     GNYSYVQTIVLDAAAGSGHRRELLQAGIEFLGPADRRVETMIGLIADIRKDAIIVYGD
                     TSFLRALLSGLSESDFAAAMKILDRKDIQGLRSFCEARSLQNSTAQILCELPFLSGAS
                     LDPLKQAAAGRSDLLEIIAAAPRFSQIVYDFGLVRDLSYYTGPVFQGYLPGGHEPFVS
                     GGVYDGLFEKFSGRPLAACGLAFDVMLLTGPNQGR"
     gene            9574..10617
                     /locus_tag="PKK76_00930"
     CDS             9574..10617
                     /locus_tag="PKK76_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67054.1"
                     /translation="MKHWFWKTILGWILLFFVILPAVLIPTLYLGKTLGSLYLSETAP
                     AAKIQGLDILKVLSAQAEGDLKPDKLVAISGTMSDVVQRMNTGATPLGIKEIALIDPK
                     GRVLAHNDVTFIAKDAVSPYANDKFLAVLRRPRRDSATVVPLQPYSPALPQNIVAEKL
                     GDRILTPLRSAYPDLFIASYTISSAVYPIDGEAPNAGFIMIVENHAPERFFSILGALL
                     SPVALVTFGSILVAMFFALPVLLLVTARGKQIVYRDHLDDDLPVAHAHAEPETHESID
                     FEDVPLPEFMDEEAASQMAPHANPSTPPATIQHVAPYNDLPPSVSAAAAVMTPPPAHS
                     YDEILDAIPIDAD"
     gene            10621..11469
                     /locus_tag="PKK76_00935"
     CDS             10621..11469
                     /locus_tag="PKK76_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL67055.1"
                     /translation="MNIATDEQGTLQYMALQGKLDGSTASDFEKTCEHFLHMGARMFL
                     LDARELDYISSSGVGVLIKWARTLERAGGFCAVSGAGSEVKWLLDFVGLSRSYPVFSS
                     EEDARRYLTERHSGATRLSILTTRGPMPESEMRPRAIPVEIRSSAPSDSQTVTSSQTA
                     SIQSTQPAAPLRESAELPAHLETLQKSLERVIHSLERIPDSIREIREQQISIGHAKTE
                     TVRFQDGIVVDCQRCHSHLRIKKSGEHLCPKCGARFLAARDGKVQFSFKNPWPQQQET
                     LSASHS"
     gene            11427..13010
                     /gene="murJ"
                     /locus_tag="PKK76_00940"
     CDS             11427..13010
                     /gene="murJ"
                     /locus_tag="PKK76_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNL67056.1"
                     /translation="MASTARNSLRLSLVTIVSRITGLIRDHYQAVFFGTGTVSAAWEV
                     AYMLPNMLRNLLAEGVLSQAFVPIYSDSLKESEDRARRVSGVILSFLFFVLLGVVFLG
                     ALVFPFLLPLYTGRSGKDAELVIWLATVLFPFILTTSLASIFMGMANTRQSFFVPSLS
                     PVLLNLILIGGFLVLVPFHLESVVSVKWLALITIFGGVSQLAFQAWHIARNGWSPEYN
                     LNLKDPALRKIYTLMAPAVLGASIFQLNQLLDVAIASYMIPEPGAITGLRFAHRLIQL
                     PTGVVGVALSTAILPVLAKAIREDRRSEIPHELSHALSFSFFLNVPAGIGLYLLGQPI
                     IDLLFYGGNWNQASTATTWAALQFYCLGIPLYSANRILTSSFYAHQDTKSPVRILVGV
                     VIINFALNMALVPFLHQGGLALSTSICAAINFIFLYNTLKRHIPEIVLSEAIRSLARQ
                     VPAYGILAIWLLGLREYSDSIAWLGNALGFKGPRALALPLVALGAGAGGVIYVACGFL
                     LRVPELDFLRRRFRLTRGS"
     gene            13027..14628
                     /locus_tag="PKK76_00945"
     CDS             13027..14628
                     /locus_tag="PKK76_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNL67057.1"
                     /translation="MAKVQTRSISRSIQNGRLGSANGLRKRPGPGGQVGLKIIQKGKK
                     KFFEKVNPATLDILGVYPLCEAAEVDHALDAARHGFERWSKLSLRERSKHLKRFRKQL
                     VKDMNEIVDTICQECGKTEMDAMIEVMVVCEHMKYVEQKGPHFLRDEKRSTGIFVIKG
                     AYVSFHPKGVVGVISPWNYPLVLSMGPITQALMAGNSVVLKPSEVTPKTSMKIAEIAY
                     RAGIPQDVLQVITGDGSTGALLVESPKTDMICFTGSTATGRKIGEVCGRLLKPVILEL
                     GGKAPMLVFDDANLKRAVSGALWGGLSNSGQTCITIDRVFVHESIFDKFIELATVELA
                     HIRQGLRHEMPSIGSMTFPRQIEIVGDQVTDAIQRGARIISGGKKNTSYAGHFYEPTI
                     VTNHTPDFRIMREETFGPVINVLPFRTEEEAVKLANDTVYGLNAYVWTRDKKRARRVA
                     RQIRSGNLNINDVYVNYFMSDLPFGGVKSSGIGRVYAIEGLRAFADQQSVCYDRLGLN
                     KDPYWFPYTPAKQGLIYRATKLLFG"
     gene            14643..>16178
                     /locus_tag="PKK76_00950"
     CDS             14643..>16178
                     /locus_tag="PKK76_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNL67058.1"
                     /translation="MSASVIRVQGAREHNLKGITVEIPRDKLVVITGVSGSGKSSLAF
                     DTIYAEGQRRYVEPLSAYARQFLGQMEKPDVDLIEGLSPAISIEQKTTHRNPRSTVGT
                     VTEIYDYFRLLFARAGVQHCHNCGRKIQSVGVDQIVDMVQTLSEGSKLQILSPLISGS
                     KGEHKDVIEQARKDGFVRLRINGEVRDLSEDITLAKNKKHDIEIVVDRIVLKPAVRSR
                     LAEALETALEKSGGMVLVNDGHEDRVYSRKSTCTHCNLSLPELSPRSFSFNSPHGACS
                     KCDGLGVLLEFDEDLVVSDPEQSILDGAMAAWGSSKSYWFIETVHNLGKKLGFEPNTP
                     YEKIPEKIRSQILHGTPGKGKFHFEGVLPSLKRRYMETKSDDVRIWLERYMSEKPCPS
                     CKGKRLRPESLSVKFHSETIDSISEKNIRDILQFFEGLKLHSTEEKIAGRIIKEIRER
                     LSFLHNVGVGYLNLSRSAGTLSGGEAQRIRLATQIGSSLMGVLYILDEPSIGLHPRDN
                     DRLI"
BASE COUNT         4046 a         4193 c         4029 g         3910 t
ORIGIN      
        1 gcgagaaaaa ctcactgccc agagtatatg tgccaaaata ctcttcgagc gattcgaatg
       61 gtgaatgcgc gaggatcaat cgctccgtaa gattcagaat ggatctttct tttctgagat
      121 gagagaaatt gtatttttcc gggaacagtt tttccttcgt gcgaagagac ctccaccatt
      181 gccagagcaa ataacgtcca agcattctgt gttcatccat gctcgccgtg gatcgatgcg
      241 gatcaagagg tggactgatg gccgccaccg cttttagatt tacgattgga gtcttcacgc
      301 gagaattgcg tgctgcaatt cgcagtgcga aattcccacc cagggaaaac ccacccagat
      361 aaactgaaaa gccgggaaac atctttgcaa tctgtctgac tgcttcaaaa tgttcgtcca
      421 gcatactccc attgaaaagt tcttcgttta gatgatgagt ggcgccatga tctctgaggt
      481 gaagccgggc aacatagaag ccgtgatcac tgagtgcctg cgaagtgcga agcatatagg
      541 gcgcctcaat gtggccctcc catccatgaa tgagaatcac aatggcccgt cgggtaccgc
      601 gactgagtgc cgcgtgaatg cgaatcccgt ctacctgcag aatgatgtct tctgcggatg
      661 cgagcaccgg atgctttcgc ttcactccga acggaatcgc aggcagaagg gtctggatcc
      721 aggcgggcct gaggtaagaa ggcggatcaa agaacgccat ggtgtagctc tctattcggg
      781 gctaccaggt cgagcttttt caacgaccct cgtccggggt tttgttttgt ctttacgaaa
      841 gaaacccacg cattgtgaat tcgtcatgcg gcctgatata ttgtctcatt cgaacctgct
      901 tgatgaggaa atccattcca ctttcaaaac cggcagcctg tattcggctg tcatggggtt
      961 gtcgagtgcc tgcctgttgt ccgccatgca atggacgggt atgattggtt ctattgaagt
     1021 gcccattctc tggactgtgt ttggagggct gtattctctc ggagtgtttc tgattgcccg
     1081 tgctcgcaaa ctcaaggggc tatggattcc gtttgcactc attggattta ccacgctgcc
     1141 cacctctatt tacgtcatca gccactgggt ccttccatct ggagcagcca catacatcac
     1201 ggggccgcct tcctatcttt atttttgcat gatcgcagtc agcggcttcg cgtttgatcc
     1261 tagaatttcc acgatcgccg ggatcgtctc cgcccttcag tatgtggggg cctttctcat
     1321 agaccgggat catttgcgca taacgtccca ggatccattg atgttgcagg acctgacatc
     1381 tacgtccatc tacttattca aaggaatgat gatggtattc accggccttg tagtcgcagt
     1441 tcttgctacg actgcgaaga aactcatcca gggaatcgtg gcggaggaaa aagagaaatt
     1501 ctctatcaaa agagtactcg gcgaatacgt ttctcctgaa gtggcagaca agattgtagc
     1561 gtcccagaag atcgcagaaa aaaaacaggt ggcgattcta tttagcgaca ttcgcggctt
     1621 cacatctttc agtgaacaca acgaaccgga cgcgatcgta gaaaggctca acgaatattt
     1681 ctcggccatg gtgagcgcca ttgaaaaaaa cggtgggacc gtggacaaat tcatcggaga
     1741 tgcagtgatg tctgtgtttg gaggagtctt gcctctggaa aacccttcgg aagcagctct
     1801 gaacgccgct cgccagatga gggcatcgct caaggaattg cagaggggat gggccagcac
     1861 gggccaacag gtctttgaca acggcattgg gatccatttt ggagaagtct tacaggggac
     1921 tctcggatca gagggcagaa aggatttcac agtcatcggt gacgcggtca acactgcttc
     1981 gcgcctggaa gggctctgca aagaaacgaa cactggaatt ttgatttccg cccaggcggt
     2041 ggagcgaatt cctgaatctt cgcgggttgg tttgagaagt ctcggaaaat ttcaggtgaa
     2101 aggcaaaatc aaggaaattg aaatctatgg cgaataacgg tatttctaaa gctaccatgt
     2161 cttttctgaa agatctcaaa aagaacaatg accgtgcctg gtttgagaag catcgggatc
     2221 gcttcgaggc ggcgcacgaa gagttttcac aaacagccca gggattgatt gaacagatcg
     2281 ctcgcctgga tcccggcatt cgtcctgtgt ctctcaaaga ctccatattt cgcatttatc
     2341 gggatgttcg tttcagcaga gacaaatctc cctacaaacc gcacttttcg ttctttgccg
     2401 cacgcggagg aagaaaatcc aaatacgctg gatactacgt gcacctggaa ccaggatcct
     2461 gtttcattgg agggggcatc taccatccgg aaccggaaaa tcttatgaag atccggacag
     2521 ccatcgcagc gcgaggggaa gagcttcgca ggattgtgca ggctgtaccg tttcgtaagt
     2581 actacaaagg attggaagga gatcgactca agaatcctcc gcgccagtat gcgaaggatc
     2641 atcctctggc ggaatttctg tgcttcaaag acttcattgc aagtgcgaaa ttgaaagata
     2701 cggaagtgac gggagatctt gccggtgctt gcatcatgcg ctttcgcgct cttctaccgt
     2761 ttaatcggtg gctttcgcgc gccgtggacc aggattcttg acgctgcttt ccagggccgc
     2821 gcggattcta gcatgaactg agtctttcat catctcgagt tttccaaacc cgggcagtgc
     2881 tcccagagag accgcttctt cctgtgcatc cggataattc gagatcagca tcaccggaat
     2941 ctcagcagtg gagcgatctg ctttcatcat gcgaattaga tccgtcccgt ctgagtaatc
     3001 tgaatcgagt ttgcgattga tgactacgag atcatacctt tcgcgcttca tggcttcgag
     3061 cgcgtctgcc ggcaaatagg ccgcatccac ctctgctccc agactttcca ttacatagcg
     3121 gatagccgca tgatccggcc tgcactggcc tacatcgaga acgcgcttct tcgccataca
     3181 ttaccatgta aatcatcgaa cggcagagag tcgataaaaa ctactgttcc tgctatgcat
     3241 gtcaaaaatc gtcggtcatg tctcttcagt gcggtatcgt tggactgccc aacgtaggta
     3301 aatctactat atttaatgca atcacccgag caggcgctca gtctgcgaac tatccatttt
     3361 gcaccatcga accaaacgtt ggacgagtcg acgtgccaga cacaagactc accaaactca
     3421 gcgacatagt aaaaccaaag aatgtggtgc ccgcggccat ggagtttgta gatatcgcag
     3481 ggcttgtaga aggagcaagt aaaggagagg gccttggcaa tcagtttctt tctcatatcc
     3541 gcgaaacaga agcagtcgcg cacgtggtcc gttgcttcac ggatgacaac atcattcatg
     3601 ttcgcgggaa agttagcccc gcggatgaca ttcgcatcat caatctggaa ctgattctcg
     3661 cagacctgga ttcgctcgag aagcaaattc aaaagatcga aaagaaagcc aaatccggcg
     3721 acaaggtaga gaaggccagg tttgatctgg ccgcgaaaat tcgcgatcat ctcaacgagg
     3781 aaaaacctct tcgcaccatg agcctttccg acgacgaaaa ggccattgcc aaaacattcg
     3841 cactgatcac cgctcgcccc atgctctacg taatgaacat ggacgaagcg tccgcatctt
     3901 ctggaaacga atacactgcc attgtggaaa agatcgcggc agaagaggga gcgcctactg
     3961 ttcgactctg cggaagaaca gaagaagaaa ttgcagctct gtccccggaa gaagcacaag
     4021 aatacctttc gtccctcggg ctcgaggaat ctggactggc cagaatgatt cgagcatcct
     4081 acaccctgct caatctactt acttacttca cggcgggaga agtggaagtg cgcgcatgga
     4141 ctgtgactaa aggagccaca gcaccggaag cggccggcgt gatccactct gatatacaga
     4201 gaggattcat ccgcgcggaa gtcatgtcct gcgacgacat tttcaaactg ggatcccagc
     4261 aggcagtcaa ggatgcgggg aagatgcgcc tggaaggaaa agactatatt gttcaggacg
     4321 gggatgtttg ctatttccgt ttcaacgtgt aaccatgcaa aggtgagatc ggaggggcaa
     4381 tgatctatcg cttcgaccaa cacacgccgc aaattgcccc tgatgttttc atcgctccgg
     4441 acgcgaccgt cattggccag gtaagaattg gagcaggcag tggcgtctgg tttgggtctc
     4501 tcgttcgcgg agacgtgcac tccatagaca taggggagca cacaaacatt caggatcact
     4561 gcgtcctcca cgtaaccgga ggaaaatttg ctctgcgaat cggaaatcat tgcactctgg
     4621 gtcatagagt cacccttcac ggttgcactt tgcatgatca tgcattcatg ggaatcggct
     4681 caacggttct tgacggttgc gtcattgagg agtttggttt tttggcggcg ggaagcctgc
     4741 ttccacccgg gaaaacaatt ccatctcgca tgcttgcgat gggaagcccg gcaaaggtag
     4801 tgcgcgaaat tacagcggaa gaagaagaaa tgatgcgcag aactgctgtg aaatacagcg
     4861 aactggcgcg ggtgtatcgc agtgcaaact ttggacccgt ctgacatcca gaaacgaagt
     4921 gatcgcacgg agctgcgcat atttcagggc ggggcagtgg tcgtgtttct ggccggtcta
     4981 ttcctgccgc tcacacgttt tccttctatc tgcatctttc tctacatcac cggacttccc
     5041 tgtccggggt gtggtctaac gcgctcgatt catttgactc tcaacggcaa gatcctgcag
     5101 gcgctgcagt ttcatccctt cgggctcccg gcacttgcct ggctttgctt cctgatactc
     5161 agtattttta taccatctct atcccgattc tacgtaaaac atattaagca gatccataga
     5221 ctgtacgcga caggtgctct cttgatgctc gcattcggcg tagtacgact gatcgggctt
     5281 ttgatgtttg gcggcctgag ctgggttgct ccttctgtct tgcgatagca gaagacttga
     5341 ccccggcggt ccaaaagcaa agtacggtta tggaacaaac attgatcatt ttgaagcccg
     5401 atgcagtgcg aaacaagcat gtcggaaaca tcatcgcacg cattgagaag gaaggcttca
     5461 atatagtagc tctcaagaag attaccctca aagacaaaga cgcaaagaaa ttttacgccg
     5521 ttcacaagga acgcccgttt tacaaagatc tctgcaaatt catgaccaca ggtcctgtgg
     5581 tggtagcggt tctagaagca cctggcgcgg tagagaaatg gcgcaaactc attggagcaa
     5641 cagatccagc ccaggcagaa gcaaacacga ttcgcaagct ctatgcggaa agcaaagaag
     5701 caaacgcagt tcacggatct gactccgaaa agaatgcgaa aggagaaatc aaattcttct
     5761 tcaaagcagc agagctcaag ggcaaactct gattgtcaga cagtgaaatc tctctcttaa
     5821 gagaggaatt cgaacaattc ctcggagaag ccggggaaat attttcaaat ggtacggtcg
     5881 ccgatctttc ggcgcccgtg ctctacagtc tcagtgcacc gggcaaacgc ctcagacccg
     5941 tactgtgcat gatgtttgga ggctatcaaa acaagacaca atcacgtggc cttctgtttg
     6001 cggccgcagc actcgaatgc attcatactt actctctgat ccatgatgac ttacctgcca
     6061 tggacaatga cgatttgcgc cgtggaattc caacctgtca caaaaaattc agcgaatggg
     6121 cagcgatcct tgccggtgac gcgctcaata cgttcgcctt tgagctgtta gctgaagcat
     6181 acgagcccgt tcttcttcca gaggcagtga aaactctggc gcgaaagggt ggtcgagtcg
     6241 ggatgattgc cggacaggca ctcgatttga aaaatgagcg aggttccagt tcgcaagaat
     6301 cgccatcgaa cgcaaacctg caggatactc tgcaaaacat tcatctgcga aaaacggccg
     6361 cactcatcca ggcgtcgtgc gtccttggct ctctgtatgc aaaaactgaa acggaatctg
     6421 cgtccggatt cggggaaacg ctcggcttgc tcttccaggt atcagacgat ctcctggatg
     6481 cgcgcggttc gaatgatgcg ggcaaagcaa ctttgaaaga cgcgaaagca ggcaagctca
     6541 catacccgtt cctatacgga atggaagagt ccatgcgaat ttgcgcggac ctggaaacac
     6601 gcgccctttc ttacctggcc cgggccccga tcagcgaaac cagacgtcat gcgctcgcag
     6661 aattgacccg gtcactcaaa gacagaacca gatgaaaggt cggggagcgc gcaagctaga
     6721 gctcgctctg gaacaaattc atctacctat ccaggattca tactgcattg atctgggtgc
     6781 gtcccatggt ggatttgtgc gaacgctgct tgaaaacggt gccgccaggg tgagcgccgt
     6841 ggacgtggct tacggaattc tcgattacag tcttcgtgtg gatccgcgcg tgagtgccat
     6901 cgaaagatcc aacgtgagaa acatagaacc atcctggttc gaagatcttc cttcttctgg
     6961 aaagatcata gttacgtgtg acgtgtcgtt tatgtccatt cgaacggtac ttgcatctct
     7021 cgcggacttt ctgcatggaa ctggacttgc catggaagga ttgttcctgg taaagcctca
     7081 gtttgaagac tctaagagta ctgtaaaagg aatcttaaaa gatccgattc gccgcgaaga
     7141 gatcatctct gaaatcagaa gcttctgcgt gacgaaagga ttcgaagtga aaggaagaat
     7201 ggatttgccc gaaatctcgg gcaaaaacct ggaagtgttc ctgcaccttt cctacgaaaa
     7261 gtagatcaaa gagggattgt gtgttcgcgg cctggtccaa ctgaaactag cttgataggc
     7321 acttcgcaaa gctcttctag cctgcgaatg tatttgcaac actcaggcgg caggtccgaa
     7381 tatttcaaaa catgaccgat ttcttttttc cagcccggca tttcctcata gactacttcc
     7441 actccctcca ggtatccatc cgtcggaaat gcagggagct tttcaccacc acgcttgtaa
     7501 cccacaccga tcttgatggt atcgtacgca gagagcacat ctatcttcgt gagtgcaatt
     7561 tcagtcagac cgttcacacg cgcagcgtgg cgcacgagtt ccacatcgaa ccagccgcaa
     7621 cgacgagggc gacctgttgt ggccccgtac tcatgaccga gctctctgag cttttctact
     7681 tctgcgccaa acaactcggt tggaaatggt ccttctccca cgcgcgaaac gtaagctttt
     7741 gaaatgccag tcacagtttt cagaaaacga aaatcaatgc cactgcctgc aatcgcaccg
     7801 ccagtcgtgg tattgctcga ggttacgtat ggataagttc caaaatcgat atcgagtccg
     7861 gttccctgag cgccttctag caacactgtt ttaccttttg ccagattctc attgagaaga
     7921 agcgaagtgt tctttaccat cccgcgaatt tcgtttccga aagccagaag gtcttcatag
     7981 agagcgttga attccacttc aggcaatcta taaagattct ttagttcata gtttttgatt
     8041 tcaagaatgt gccccagtct gtctctcagg cgatccgcat gcagcacatc gccggcgcgc
     8101 agaccaattc gcatggtctt gtcaccgtag cagattccaa ttccacgctt cgtggttccg
     8161 atcttttttt cacctgccgc attctcgcgg tgagcgtcta tgattctgtg aagaggaaga
     8221 accagatgac atgcatctga aataataacg cgttcttttg cctctatgtt tcttctggca
     8281 agagcgcgca cttcatcgcg aaacgcttcc gggtcaagca cgacgccgtt tccaatgacg
     8341 cacactgcat ttggatagag aatgccgctt ggaatcaaat gaaagatgta ggtttcgccg
     8401 tccacaacca cggtgtgtcc tgcattcgcg ccgccctgat agcgaacgat aaaatcaatc
     8461 ttagaactca ggtaatcgat tacctttgcc tttccttcat ctccccactg tgttccaact
     8521 acgagagcag cgctcatgat ccctactttg cctcaaattc ggaggctatt ttatattatc
     8581 ggccctgatt gggaccggtc aacagcatta cgtcaaatgc aaggccacag gcagccagag
     8641 gcctgccaga aaacttctcg aacaaaccat catagactcc tccagaaaca aaaggctcgt
     8701 gtcctcccgg aaggtagccc tgaaacacag ggcctgtata gtaagaaaga tctcgcacca
     8761 gcccaaaatc atagacgatc tgcgaaaacc gcggagccgc agctataatt tcaagtaaat
     8821 cggaccttcc tgccgctgct tgcttgagag gatcgagaga tgccccggaa aggaaaggga
     8881 gctcacagag aatttgcgcg gtggaattct gcaatgatct ggcttcgcaa aaactgcgca
     8941 gtccctgaat gtcctttcgg tctagaatct tcatggccgc ggcgaagtcc gattcgctga
     9001 gtccagatag aagtgcgcgc agaaacgatg tgtctccgta cacaatgatg gcatcctttc
     9061 gaatgtccgc gatgaggcca atcatcgtct cgacgcgacg atctgctggt cccagaaact
     9121 cgattcctgc ctgaagaagc tctctcctgt ggcctgagcc ggcggctgcg tccaggacaa
     9181 tggtttgcac gtagctatag tttcccggct ggccaaaaac cccggaagcg gctgccttga
     9241 tgatctgaac cgtcaaatcc gagcgaaaag caaggagctc gccggccata tccctggtct
     9301 ggaagcttgc catttcgctt tgcctggcca tttcgaaggt gggggcgaag tcgagcaggg
     9361 gtggaatgac ttctctggca ccaagagccg cgaatgcttc tcttagcctg gcgcatccgg
     9421 cgagaattcg ttccgtctga tcgggcccga tgaattccat cccatgggga atccagcttt
     9481 taaagcgctc tttcagctca ggattcttca cgatagccag tttccttatg atctattccc
     9541 ccgcaaatac cgattttaaa gaaaggcccg aatatgaaac actggttctg gaaaacaatc
     9601 ctggggtgga tcctactgtt ctttgtaatc ctccctgcag tcctgatacc cacgctctat
     9661 ctgggcaaaa ccctgggttc gctctatctc tctgaaacgg cccccgcggc aaagattcag
     9721 ggactcgaca tactcaaagt gctctccgcc caggcagaag gagatctcaa gccggacaag
     9781 cttgtggcga tctccggaac catgagcgat gtggtgcaaa gaatgaacac cggtgccact
     9841 ccgcttggga tcaaggagat agcactgatt gacccgaaag ggcgtgtgct tgcgcacaac
     9901 gatgttacat tcatcgcgaa agacgcagtg tccccgtacg caaatgataa gtttctcgca
     9961 gtgctcagac gtcctcgccg cgattcagcg accgtagttc cgctgcagcc atattcaccg
    10021 gctcttccgc agaacatcgt cgcggagaaa ctaggagata gaatcctcac tccgctgcgt
    10081 tctgcttacc cggatctctt cattgcctcc tacacgattt catccgccgt ctatccgatt
    10141 gacggagaag cgcctaacgc tggattcatc atgatcgtag agaaccatgc accggaaaga
    10201 ttcttctcca ttctcggagc tcttctttct cccgttgctc tggtaacgtt tggatcgatc
    10261 ctggtcgcaa tgtttttcgc gcttcctgtg ttactccttg tgactgcacg tggaaagcaa
    10321 atcgtatacc gcgatcacct ggatgatgat cttccagtcg cgcatgcgca tgcagaacct
    10381 gaaacgcatg agtcgattga tttcgaggat gttcctctgc ctgagtttat ggacgaagaa
    10441 gcagcgtcac aaatggcacc ccacgcaaat ccttccactc cacctgcgac catccagcac
    10501 gtagcgcctt acaacgattt gccgccctcc gtctcagccg cggcagctgt gatgacgcca
    10561 ccacctgcac attcttacga tgaaattctg gatgcaattc caattgatgc ggattgagat
    10621 atgaacatag ccacagacga acagggtaca ctgcaataca tggccctcca gggaaaactg
    10681 gatggatcga ctgcatctga ctttgaaaaa acatgcgaac actttctgca catgggtgcg
    10741 cggatgttct tactggatgc acgcgaactg gattacatat ccagctcggg tgtgggtgtg
    10801 ttgatcaaat gggcgcgcac tctggaaaga gcaggcggat tctgtgcggt gagcggtgct
    10861 ggttctgaag tgaagtggct tctggatttc gtaggtctct ctcgttccta tcctgtcttt
    10921 tcttctgaag aagatgcaag aaggtatctc acagagagac attctggagc gactcggctc
    10981 agcattctta caacgcgagg tcccatgcct gaatcagaaa tgaggcctcg cgcgattcct
    11041 gtagaaattc gtagctccgc gccttctgat tcgcagactg tcacttcatc gcagactgct
    11101 tccattcaga gcacacaacc ggcggcccct ttgagagaat ccgcagaact tcctgcacat
    11161 ctcgaaacac tgcaaaaaag cctcgaaaga gtgattcatt ctctggaacg aatcccggat
    11221 tcgatccgcg aaatccggga acagcaaatt tcaatcggcc atgcgaaaac agaaactgta
    11281 cgtttccagg acggaattgt agtcgactgc cagcgttgcc attctcactt gagaattaag
    11341 aaatctggag aacatctctg tccaaagtgc ggagctcgtt ttctggcggc cagagacggc
    11401 aaagttcaat tctcatttaa gaatccgtgg cctcaacagc aagaaactct ctccgcctct
    11461 cactcgtaac gattgtatcg cgaatcacag gcctgattcg cgatcattac caggcagtct
    11521 tctttggaac cggaacggtt tccgctgcat gggaagtggc ttacatgctt cccaatatgc
    11581 ttcgcaatct cctcgcagaa ggggttcttt cgcaagcatt cgttcccatt tattcagact
    11641 ctctgaaaga atcagaggat cgcgcgcggc gcgtgagcgg agtcatactc tctttcttat
    11701 tcttcgttct tcttggcgta gtgtttctgg gagctcttgt gtttccgttc ctgcttccgc
    11761 tctacactgg aagatccggc aaagatgcag agctcgtcat ctggcttgcc actgtactct
    11821 ttcctttcat tctcacaacc agcctcgcgt ctatattcat gggcatggcc aacaccaggc
    11881 agtctttctt tgttccatct ctttctccag ttctactgaa tctgattttg attggtggat
    11941 tcttggtact ggttcctttt catctggaaa gtgtcgtgag cgtgaaatgg ctggcactca
    12001 ttaccatctt tggaggagtc tctcagcttg cgtttcaagc ctggcacatt gcccgaaatg
    12061 gatggtctcc cgaatacaat ctgaatttga aagatccggc ccttcgaaag atctacactc
    12121 tcatggcccc tgcagtcctc ggcgctagca tcttccagct caatcagttg ctcgacgttg
    12181 ccattgcaag ttacatgatt cctgaacctg gagccatcac cggtttacga ttcgcacatc
    12241 gcctgattca attgccaact ggagttgttg gagtggctct ctccactgcc atccttccag
    12301 ttctcgcaaa agcaatccgc gaagatagaa gatctgaaat tcctcatgaa ctgagtcacg
    12361 ctctgtcttt ctcttttttt ctgaacgttc ctgcgggaat cggactgtac ttgctcggtc
    12421 agcccatcat cgatcttctc ttctatggag gaaactggaa tcaagcgtct accgccacaa
    12481 cctgggcggc actgcaattc tattgcctgg gcattcctct ctacagcgcc aatcgcatcc
    12541 ttacttcttc cttttacgca catcaggaca caaagtctcc tgttcgaatt ctggtgggtg
    12601 tagtgatcat caacttcgcg ctcaacatgg ctctcgttcc attcctgcac cagggcggcc
    12661 tggctctcag cacttctatc tgtgcggcca tcaatttcat attcctctac aacactctca
    12721 agcgccacat tcctgaaatc gttctctcag aggcgatccg gtccctggcc cgccaggtgc
    12781 ctgcctatgg aatactggct atctggcttc tgggcctccg agaatacagc gattcgattg
    12841 cctggctggg taatgcactg ggtttcaaag gaccaagggc actggcgctt cctctcgttg
    12901 ctctgggagc cggggcgggt ggtgtaatct acgttgcctg cggctttcta ttgcgcgtgc
    12961 ccgagctcga cttcctgcgt cgtcgatttc gcttgacgcg tggttcctga gagtcgtcct
    13021 ttaaggatgg caaaagtcca aacaagatcc atctccagaa gcatccagaa tggccgacta
    13081 ggaagcgcaa acggccttcg caagagacca ggaccaggcg gtcaggtcgg actcaagatc
    13141 atccagaaag gcaaaaagaa attctttgaa aaagtaaatc ccgccactct agacatactc
    13201 ggagtatatc cgttatgcga agcggcggaa gtagatcatg cactcgatgc ggcccgtcac
    13261 gggttcgagc gctggagcaa gctcagtctt cgcgaacgct ccaaacatct gaagcgattc
    13321 cgcaagcaac ttgtcaaaga catgaacgag atcgtagaca cgatctgcca ggaatgcggc
    13381 aaaacagaaa tggacgcgat gatcgaagtg atggttgtct gcgaacacat gaagtatgtg
    13441 gagcagaagg gcccgcactt tctgcgcgat gagaagagat ccactgggat cttcgtcatc
    13501 aaaggagcat acgtcagctt tcatccaaag ggtgtagtgg gagtcatctc tccgtggaac
    13561 tatcctctcg tgctttccat gggcccgatc actcaagcac tcatggcagg caactcagtc
    13621 gtcctcaagc catctgaagt tacgcctaag acaagcatga aaattgcgga gatcgcctat
    13681 cgtgctggaa ttcctcaaga tgttctccag gtcatcacgg gtgatggaag cacgggtgct
    13741 cttctggtgg aatcgcccaa aacagacatg atctgtttta caggctctac agcaactggc
    13801 agaaaaatcg gagaagtatg cggtagattg ctcaagccag tcattctcga gctcggtgga
    13861 aaagcaccca tgcttgtatt cgatgatgca aatctaaagc gtgcagtgag cggtgctctg
    13921 tggggaggtc tttccaactc cggccagact tgcattacca ttgatcgagt cttcgtgcac
    13981 gaatccatct ttgacaagtt catcgaactg gcgactgtag agctcgctca cattcgccag
    14041 ggtttgcgtc atgaaatgcc gagcattggc tcgatgactt ttccccgtca gattgaaatt
    14101 gtaggagatc aggtgacaga tgcaatccag agaggagcgc gaatcatcag cggcggaaag
    14161 aagaatacgt cgtacgccgg acacttctac gaaccaacga tcgtcaccaa tcatacgcct
    14221 gatttcagaa tcatgcgcga agagacattc ggcccagtca tcaatgtgct tccatttcgc
    14281 acggaagaag aagcagtcaa actggcaaac gacaccgtct atggcctcaa tgcgtacgtc
    14341 tggacccgcg ataagaagcg agccagacgc gttgcaagac aaattcgcag cggaaatctg
    14401 aacataaacg acgtctacgt aaactacttc atgtctgatc ttccgtttgg aggagtcaaa
    14461 tccagcggaa tcggaagggt ttatgcgatt gaaggcctgc gtgcctttgc agaccagcaa
    14521 tccgtatgtt acgatagact cgggctcaat aaggatccgt actggtttcc ttacactcct
    14581 gccaagcagg gcttgatcta tcgcgccacc aaacttctgt tcggttgagt agacctgctc
    14641 gtatgagtgc cagcgtcatt cgagtacagg gagcgcgtga gcacaatctc aaggggatca
    14701 cagttgagat ccccagagat aagctcgtag tcatcaccgg cgtttccggg tcaggcaagt
    14761 catctctcgc tttcgatacc atctatgcgg aaggccagag acgttatgtg gaaccacttt
    14821 ccgcatatgc caggcaattt ctgggtcaga tggagaagcc agacgtggac ttaatcgaag
    14881 gattgagtcc ggctatctcc attgaacaaa agacaactca cagaaatccg cgatccactg
    14941 ttggaacagt tacagaaatt tatgattatt ttagactatt gtttgcacgc gctggagtgc
    15001 agcattgtca caattgcgga aggaagatcc agtctgtagg cgtagaccag atcgtagata
    15061 tggtccagac tctctccgag ggaagcaaac ttcagattct ttctcccctg atatctggga
    15121 gcaaagggga gcacaaagac gtaatcgaac aggcgcgcaa agatggcttt gtgcgactgc
    15181 gaatcaacgg agaagttcga gatctgtccg aagacatcac cctggctaaa aacaagaagc
    15241 acgatattga aatcgtcgta gatcggattg tcctcaagcc ggccgtaaga tccaggcttg
    15301 cagaagcgct cgaaaccgct ctcgaaaaaa gcggtggaat ggtcctcgta aacgacggtc
    15361 atgaggatcg agtctactct cgcaaaagca cttgcactca ttgcaatctg tctcttccgg
    15421 aactgagtcc gcgaagtttt tcattcaatt ctccgcacgg tgcctgcagc aagtgtgacg
    15481 gcctgggagt cctccttgaa tttgacgaag acctggtagt gagtgatcca gagcaaagca
    15541 tcctggacgg agctatggct gcgtggggtt cttccaaaag ctactggttc attgaaaccg
    15601 ttcacaacct gggtaaaaag ctgggattcg aaccaaacac tccatatgaa aagattccgg
    15661 agaaaattcg ttcccagatt ttgcatggga cacccggaaa aggcaaattc catttcgagg
    15721 gtgtgcttcc ttctctcaaa agacgctaca tggaaacaaa gtcggatgac gttcgcatct
    15781 ggctcgaacg ctacatgtct gagaagccgt gtccttcctg caaaggcaag agactgagac
    15841 cggagtctct ttccgtgaag tttcatagcg agaccattga ttccatttcc gaaaaaaaca
    15901 ttcgggacat cctgcagttt ttcgaaggcc tcaaacttca ttcaacagaa gagaaaatcg
    15961 caggtagaat cataaaggaa atcagagaga ggctttcttt cctgcacaac gtaggtgtgg
    16021 gctatctgaa tctgtccaga tctgctggaa ctttgtctgg cggagaagcg caacggattc
    16081 gcctcgcaac tcagataggc agttctctca tgggagttct ttatatcctg gacgaaccat
    16141 cgatcggtct gcaccctcgc gacaacgatc gcctgatc
//
LOCUS       DAORFF010000011        16157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1490_length_16157_cov_3.555894, whole genome shotgun sequence.
ACCESSION   DAORFF010000011 DAORFF010000000
VERSION     DAORFF010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 16157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16157
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1490_length_16157_cov_3.555894"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..369
                     /locus_tag="PKK76_00955"
     CDS             172..369
                     /locus_tag="PKK76_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67059.1"
                     /translation="MWDRDDEDLEEDLDWDEDVEDDEDSEHASFVCEDCDYRWDESQE
                     EDMDTGMHVCPMCGSVNVTQL"
     gene            371..598
                     /locus_tag="PKK76_00960"
     CDS             371..598
                     /locus_tag="PKK76_00960"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNL67060.1"
                     /translation="MLPLRLTFMLIGALILALLAFGNPEPVPIRFLFWTDEIALYKLL
                     LSAVLFGIILAVLYIGHVRYLRRHLGDRFRR"
     gene            598..1143
                     /locus_tag="PKK76_00965"
     CDS             598..1143
                     /locus_tag="PKK76_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNL67061.1"
                     /translation="MFRVQKGTLKGKSISMPPESRGHSCFTPGLVKEALFQLVENRLE
                     TEPSDYAFFDLCAGSGQIAIEALSRGYHPVHAAELDQGRFEFLIRQVRAQKYDIRLHR
                     KDFRKMSPFIEGRSVVFLDLPYTFWTPLPALITDFLGSLREPAPGGHRIIVIQGPAAW
                     PDWEPHPYGGTVLSLSEQKMV"
     gene            1241..2794
                     /locus_tag="PKK76_00970"
     CDS             1241..2794
                     /locus_tag="PKK76_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNL67062.1"
                     /translation="MDIIALIIIALYSIDIALLFFFGIHSYIMVYLYRKNEKYCVSGQ
                     DVHMDPIDISKTPESEIPVVTVQLPVFNEFYVVDRLIEATTQMVWPKSKLEIQVLDDS
                     TDESKDKAATLVEMYRSRGFDIHHIHRTNRQGHKAGALKEGLEVARGEFVAIFDADFL
                     PASDFLVKTVPYFQEPGIGMVQTRWGHINDDYSVLTKAQSFGIAGHFMIEQVARNCNN
                     LWMNFNGTGGIWRRQCIVDAGNWQSDTLTEDFDLSYRAELAGWSFRYFKDIVNPAELP
                     ATMSAFKSQQFRWCKGSIQTAVKLIPTILRSKFSRKIKSEAIVHLLNYSVHPLMMINI
                     LLCLPLLLMDVWTGFSIYDLSLAVLFGAATLMSLGTFGPVVFYIYSQRELYPDWKKRI
                     WWMPVLMMIGTGIAVVCGRAFLEAILGIQSTFKRTPKYRIENKSDKLIERMKYKLPLD
                     KTVFIEFFMGFYCVACIYFSIVKNKPFVVPFMLIYAAGFFYVALSSLSESFVRVKRPA
                     IDSAADARA"
     gene            2781..3647
                     /locus_tag="PKK76_00975"
     CDS             2781..3647
                     /locus_tag="PKK76_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67063.1"
                     /translation="MLEPDRWPTLEFSLTGMSLLSGGECFVSPFPCSFPSSRVNLLEQ
                     AELDLCRGRKVDSLNWLVPNGAESFFDFVFEQAQKGLRVRGDSAAPIEAIHSGGKSWA
                     ERFPILHVSEHSTIPSRESARPVSRFIYLNPEGEALPFARREAESFCNLFPDARAAFR
                     TLGEGEFNEAMADATHIYYFGHAMMVDGSPAIPARDAWIPLLSRSMGDVSRKTVFLGA
                     CLRQAGMTLRIPGGRFIYPITRIADRPNSFLERIAGSSHLLQGFHSACIRDAEEGDIR
                     RFVYRVKGSIWL"
     gene            3692..4051
                     /locus_tag="PKK76_00980"
     CDS             3692..4051
                     /locus_tag="PKK76_00980"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNL67064.1"
                     /translation="MDAAVRLRAPNQQKRKKARAQVNLPADFQILGHHEFLPCSLADI
                     GSGGLALITRSSLYIGDKLKVRFKIAESPFEGQCVVVRVVGKAVGVQFENLSPADEDR
                     LQDFIHKVFLERERKKP"
     gene            4100..4384
                     /locus_tag="PKK76_00985"
     CDS             4100..4384
                     /locus_tag="PKK76_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67065.1"
                     /translation="MEKVYCANCAHCIVVRQYEAEQDKYILRVRCDKKKWSKRSGEEK
                     LYKYFTVARRMQPNCEYYRDMGDLLPYIKNLKKELPIKDEIYSVKKSARP"
     gene            4406..4873
                     /locus_tag="PKK76_00990"
     CDS             4406..4873
                     /locus_tag="PKK76_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNL67066.1"
                     /translation="MELKDIIPPDRIFYIERSFKSKEELLSHLLEQLLKKDGLEAHKD
                     QVLKVLLERESSMSTGIGQGIAIPHCSTDFEKDLSAVLAVLKKPIEFQAVDEQPVQIV
                     ILLVLPKDRFEKHIKTLAAIARQFNDSVFRKKVAGAASVDEVQAAVAAATASA"
     gene            complement(4877..5452)
                     /locus_tag="PKK76_00995"
     CDS             complement(4877..5452)
                     /locus_tag="PKK76_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNL67067.1"
                     /translation="MNKNPMEEFAGLQNELDKRFLDQRKIFLWGAVEDESARHIVERL
                     LYLESQAPGKDIQFYINSPGGVITSGMAILDTMNMITSDVSTICMGLAASMGALLLCA
                     GKKGKRFGFPHARVMIHQPLISGQIVAPAIDIKIHAEEIKKTRDEINRIIANATGKAL
                     EQVEEDTDRDYYLTAKEATEYGMIDGVIDRI"
     gene            complement(5483..5887)
                     /locus_tag="PKK76_01000"
     CDS             complement(5483..5887)
                     /locus_tag="PKK76_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67068.1"
                     /translation="MSRLKVQLAALGGMVVITFLLHVFLFGEAGYFTRQKLQDRLHAI
                     RGQIEELETENRTLEEEYAKIEETPAARSSAPGATILTFETNTQSDPAMFSAKMERTL
                     ADPETVFFLVMSLLSASGMVLLRMLPVGRKSF"
     gene            complement(5923..7212)
                     /gene="eno"
                     /locus_tag="PKK76_01005"
     CDS             complement(5923..7212)
                     /gene="eno"
                     /locus_tag="PKK76_01005"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNL67069.1"
                     /translation="MHTIHAREILDSRGNPTVEVDVILADGTLGRAAVPSGASTGQYE
                     AVELRDGDSNRYLGKGVSRAVDNIHKEIAPALKGRVVFEQAALDRTMIDLDGTPNKGR
                     LGANAILGVSLAAAKAAATNLKIPLYRYLGGPSVSTLPVPMMNIINGGAHADNNADFQ
                     EFMIIPREFPSFKEALRAGAEIFHALKKVLHDKGLATSVGDEGGFAPMVASNQDAMDL
                     IMTAIEKAGYKPGVNVFLGLDCAASEFCKDGKYILEAEAQKEKSPDQLIEYYESLIGK
                     YPLITIEDGLDENDWAGWSKLTQKLGKKVQLVGDDLLVTNTEKLSRAIREGIANSILV
                     KVNQIGSLTETLAAIDMARRAGYTAIISHRSGETEDATISHIAVATGASQIKTGSLSR
                     TDRVAKYNELLRIEEHLEKQANYPGKKAFFSIPGNAV"
     gene            7365..7874
                     /locus_tag="PKK76_01010"
     CDS             7365..7874
                     /locus_tag="PKK76_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67070.1"
                     /translation="MVSAQLTLESLNTVETSVETWIDFCTVSLQSDFYRLTLQKTGSI
                     ERAAALTLLNNYISTFSPSERERLERDAEFFYGYARGFIEELAPFRYNKSGYDPGVRS
                     AFMSKIKALLRAQKNPDGSYKDKERYIFVETIVRICSSLDFIIKVHDDYKEFLFRELP
                     QFRTAPNLK"
     gene            complement(7906..8970)
                     /locus_tag="PKK76_01015"
     CDS             complement(7906..8970)
                     /locus_tag="PKK76_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNL67071.1"
                     /translation="MEEQKQQDSLDLSARIVQLLFFAITIFFLFITLYGAKEVSVPIA
                     IAFLITLMLNPLVDLLEGAGLPRILATVIVFLLFIAALYFTVYNIVPLIQDQVRDIVS
                     RWPDIQKKANVFFTDTNAMLAEKLPRFIRIERLRMDTITGFIEEWSKQQGQTMKENLG
                     SIVTNLIITPIITFIFLLQGEEIYLRIIRIVPNRYFEMSLLVAHRVKKQITGYLRGLA
                     VQLLINVSIFSVGFMWIGLDYGFTMGVVAGSLNFIPYAGPVIGIIPALIIALQQGGNV
                     VMLVLVVFACAHLFDNVFTQPVILARSAELHPLIAILALITCERLFGVVGMIIAIPLA
                     GILLVTIEVMYNSLKSFRII"
     gene            complement(8970..9512)
                     /locus_tag="PKK76_01020"
     CDS             complement(8970..9512)
                     /locus_tag="PKK76_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNL67072.1"
                     /translation="MSPFWSRLSPFSAEDAVEVRLKSAPMFRDISDRGLKEIRSLCHL
                     RDYAAGEHIFREGEPGVGMYVILDGVIDIYHRDGKREKHLATLESGDFFGELALLEEL
                     PRTASAAAKNHARIIGFFRPDLMSILDRKPRLGGQVMLNMAKLIGQRLIHANMELEKL
                     DSQMSENSNQKLRKKIWQDK"
     gene            9612..9683
                     /locus_tag="PKK76_01025"
     tRNA            9612..9683
                     /locus_tag="PKK76_01025"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9644..9646,aa:Pro,seq:tgg)
     gene            9690..9766
                     /locus_tag="PKK76_01030"
     tRNA            9690..9766
                     /locus_tag="PKK76_01030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9724..9726,aa:Arg,seq:tct)
     gene            9773..9845
                     /locus_tag="PKK76_01035"
     tRNA            9773..9845
                     /locus_tag="PKK76_01035"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9806..9808,aa:His,seq:gtg)
     gene            9969..10050
                     /locus_tag="PKK76_01040"
     tRNA            9969..10050
                     /locus_tag="PKK76_01040"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10003..10005,aa:Leu,seq:tag)
     gene            10059..10130
                     /locus_tag="PKK76_01045"
     tRNA            10059..10130
                     /locus_tag="PKK76_01045"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10091..10093,aa:Gly,seq:gcc)
     gene            10164..11495
                     /gene="tig"
                     /locus_tag="PKK76_01050"
     CDS             10164..11495
                     /gene="tig"
                     /locus_tag="PKK76_01050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNL67073.1"
                     /translation="MEYKTKRLENASVQIVLKNTPEEVQQAYATAYEKAAKKVRVPGF
                     RPGKVPMEVLERELGDAVAGDAAQILVASSMEKLLGEIKPPPISVPSFEVEQFDRKQG
                     ATYRGTFDTYPEVKLGKYKKIKASQDTVEVLEEDIMAEIEKLRKDHSIMHTRDDGALA
                     GDVLTIELDIRESGGKKKLYHNKDYKMVLSEPSPFPGLVEQLLGAKSGESKSYDQQME
                     ESFPDPKFAGKLVSVTANISTVQYPVLPELNDDLAKEVSEFDTLGALKNNIRETLTKQ
                     ADNILKDRCMKTILDLIAKDSKTEMPQTLVDAEIEERVKQIARSIGRKDLTLEQLAEM
                     MGKPAPELRKELNEASVGSVKKRLVLEEIAKKEEVKVEEADILEYAEERFGTLTDDLK
                     KTLLSNEKFLDEIEGAVLFRKTFDWIHRNADIKPGEKVKLSTLMQATGTGA"
     gene            11544..12143
                     /locus_tag="PKK76_01055"
     CDS             11544..12143
                     /locus_tag="PKK76_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNL67074.1"
                     /translation="MAFMPIISEQTPRGERQYDVYSRLLKDRIVFLGYPIDDVYANIV
                     IAQLLFLEAEDPEKDVYVYINSPGGYITSGLAVYDTMQYIRPNIRTICIGQASSIASL
                     LLAAGAPGKRSALPNSRIMMHQPLGGVTGQSRDIEIQAREILSVKDKLNEIYAKHTGK
                     PLEQIEKDTDRVHHMSATEAKNYGLIDAVIESPIKLEKK"
     gene            12189..13490
                     /gene="clpX"
                     /locus_tag="PKK76_01060"
     CDS             12189..13490
                     /gene="clpX"
                     /locus_tag="PKK76_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNL67075.1"
                     /translation="MKKKTTDREKLHCSFCGKEQDSVKRLVAGPGVYICDECIELCNE
                     IIAEETDTERPVGTATGAELPRPVEIKKILDQYVIGQDSAKKALAVAVYNHYKRIEQN
                     TRKSDVDLDKSNILLIGPTGSGKTLLAQTLAKMLKVPFAIVDATALTEAGYVGEDVEN
                     ILLRLIQNAENDVKRAEIGIIYIDEIDKISRKGDNPSITRDVSGEGVQQALLKILEGT
                     VANVPPQGGRKHPHQDFITLDTRNILFICGGAFVGLDKIIERRLGSRTIGFNRDEEAA
                     RKAGEKSEILANVLPDDLMKFGLIPEFVGRLPIVATLQDLDVETLKKIFMEPRNSLLK
                     QYQKIFEIEGVKLKFTDEAITRIAELAIKREAGARGLRSIVEHQMMDLMYDIPSRDDV
                     ESVAITPGVIESGDSPVLSFKKEGAKEKEKASSSKKQEKLA"
     gene            complement(13497..14333)
                     /gene="tatC"
                     /locus_tag="PKK76_01065"
     CDS             complement(13497..14333)
                     /gene="tatC"
                     /locus_tag="PKK76_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNL67076.1"
                     /translation="MAGRKRATRARAPIQPPPTPAPAPEELPDPREGVMTLGDHLEEL
                     RRRILWSLGILAIFSIAAGFFISDIHRYISEPYHRATGLNMMLGSAMDALNTMIQLSV
                     AVGFAAGLPVVIFMLFEFVTPAMERRTAIYARISMVFSCLLFWGGVLFCWFYIFPLSV
                     EMMFKFLLPPNTQALISLDKYYSFFFILHIGTGVTFQLPLLIVILGALGVVPISLHRR
                     AWRFVIVGMFLLSMAVTPGDPVSLFVVALPLCVFYAIAVAIVYMIERNREKAAARDAA
                     EA"
     gene            complement(14333..14623)
                     /gene="tatA"
                     /locus_tag="PKK76_01070"
     CDS             complement(14333..14623)
                     /gene="tatA"
                     /locus_tag="PKK76_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004758675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNL67077.1"
                     /translation="MVLFLTNSPFDWVIILVIVLILFGGRKIPALAKDLGGGIREFRQ
                     ALLNPNQTQTTQMIEHQPQPAETYTPPPAAPRKRAAAKPAARKPAKKSRARS"
     gene            14726..15532
                     /locus_tag="PKK76_01075"
     CDS             14726..15532
                     /locus_tag="PKK76_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67078.1"
                     /translation="MGVTKEQKNLYNERVKSYKQGLDEIKKEMAMVKGMGKSPTLQPY
                     ATIRLAIESLKNATIHAQMSRLSYRILKIRRDEVLADAKKDIFNSLTDLLKLVGSDVD
                     ESLTENKERLPKIAAMTPRQKLELLKGFRSVTDYVQEAMGPSHKMRWSFPELHYKIAV
                     LGKNIMDFKEYERSKDPTEEHYQDRRALLQFIVDQCHFAAQEYRSKHELSTKEVTDLQ
                     SVRKMFELIKKIQSMTGNRTELEKITTSLDSVNEKIEAMMAEKKDKKKKI"
     gene            15586..15897
                     /gene="trxA"
                     /locus_tag="PKK76_01080"
     CDS             15586..15897
                     /gene="trxA"
                     /locus_tag="PKK76_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNL67079.1"
                     /translation="MAVVALKDDSFSQDTKEGLVLVDFWAEWCGPCRMVAPIVEELSS
                     EMPQVKFAKVNVDENQRIAQTLGITAIPTLVLYKDGQPVDRVVGLLPKPQLKNFINKH
                     V"
     gene            complement(15906..>16157)
                     /locus_tag="PKK76_01085"
     CDS             complement(15906..>16157)
                     /locus_tag="PKK76_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67080.1"
                     /translation="SRDPRIAAIVSMSAGHDHGTNVTYRLLHSGMRTFALLENQGHSH
                     AFTLTLEQAALIELGFPVTVESTSTNTHTHTVVLRKQDL"
BASE COUNT         4053 a         3947 c         4179 g         3978 t
ORIGIN      
        1 tgcttggccg ggatgagtca ttcggccttc ttcagcagta caatttgctg gaaagtcgct
       61 ttcgcagcct gcgaatcaca agaaggtcag aatgcacctg tggcagaaaa tccctcccga
      121 aataaaaaag tctggcattt tctgacggtc gtatgggata gcccatagcc tatgtgggac
      181 agagacgacg aagatcttga ggaagacctt gactgggacg aagacgtaga agatgatgaa
      241 gacagcgagc acgcaagctt cgtttgcgaa gactgcgact atcgctggga cgaatctcag
      301 gaagaggata tggacacagg aatgcatgtt tgcccgatgt gcggcagcgt gaatgtgacc
      361 cagctatagc gtgcttccac tccggcttac attcatgttg atcggggctt tgattctggc
      421 cctgttggcc ttcgggaatc cggagccggt tccgattcga tttctgttct ggacggacga
      481 aatcgcgctc tacaagctac ttttatccgc tgtgcttttt ggaatcatac tggcagtttt
      541 atacattggc catgtgcgat atttgcggcg tcacctcggt gatcgctttc gtcgctgatg
      601 tttcgcgtcc agaaagggac tctcaagggc aaaagcattt ccatgccgcc cgaatccagg
      661 ggccatagtt gcttcactcc tggactggtc aaagaggcac tttttcagct cgtggaaaac
      721 agactggaga ctgagccttc ggactatgct ttctttgatt tgtgcgcggg ctcaggccag
      781 atcgccattg aggctctcag ccgcgggtat catccagtcc acgcagcgga actggatcag
      841 ggtagattcg agttcctgat ccggcaggtt cgcgctcaga aatacgacat tcgcctccac
      901 agaaaagatt tccgcaagat gagccctttt atcgaaggcc gttccgtggt ctttctggac
      961 ctcccgtata cgttctggac cccattgcct gctttgatca ccgatttcct tggatctctt
     1021 cgtgaacctg cccccggcgg ccatagaatc atcgtgatcc agggccccgc agcatggccc
     1081 gactgggaac cacacccgta cggcggcaca gtcctgtcct tatccgagca aaaaatggtc
     1141 tgacgcggaa tcgtaaaaaa tttgaccgca aacgcgtctc aaaaaacatg ctccgaaaac
     1201 atatctagcg caaatttgcg cgaggaaccc gtcttttccc atggatatca tagcacttat
     1261 aatcattgca ctttactcga tagacatcgc tctgttgttc tttttcggga tacactctta
     1321 catcatggtg tatctctatc ggaagaatga aaaatactgc gtgtccggcc aggatgtgca
     1381 catggatcca attgatattt caaaaactcc tgagtcagaa attccagtcg ttaccgtaca
     1441 acttcctgtt ttcaatgaat tctatgtcgt agaccgcctc atcgaagcta cgacacaaat
     1501 ggtatggcca aaatccaaac tggaaatcca ggtactggac gactcaacag acgaaagcaa
     1561 ggacaaagca gccactctgg tggaaatgta tcgttcgcgt ggttttgaca ttcatcacat
     1621 ccatcgcacc aatcgtcagg gccacaaagc aggcgcactc aaagaaggac tcgaagtggc
     1681 tcgcggagaa ttcgttgcta tctttgacgc agacttcctt ccagcatctg acttccttgt
     1741 gaaaacagtt ccttacttcc aggagccagg gatcggcatg gtccagactc gctggggaca
     1801 catcaatgac gactattccg ttctgaccaa agcacaatca tttgggatcg ccggtcactt
     1861 catgatcgaa caggttgcgc gcaattgcaa caatctctgg atgaacttca acggaaccgg
     1921 tggcatctgg cgtcgtcagt gcatcgtaga tgcgggcaac tggcagtcag atactctcac
     1981 agaagacttt gatctttcct atcgcgcaga actcgcaggc tggagcttcc gttatttcaa
     2041 agatattgta aatcctgcag aacttccggc aaccatgtct gctttcaagt cgcaacagtt
     2101 tcgctggtgc aagggttcca tccagactgc agtcaaactc attccaacga tccttcgctc
     2161 taaattttcg cgcaagatca agtcggaagc aatcgttcat ttgctcaact actctgttca
     2221 tcctctcatg atgatcaaca tcttgctctg tcttcctctt ctgctcatgg atgtgtggac
     2281 aggatttagc atttatgatc tttctctggc tgttcttttt ggtgcggcca ctctgatgag
     2341 cctgggaacg tttggtccgg ttgttttcta catctattct cagcgtgaac tttatccaga
     2401 ttggaaaaag agaatctggt ggatgcctgt tttgatgatg attggaactg gaatcgcagt
     2461 tgtttgcggt cgtgcgttcc tggaagcgat tcttggaatt cagtctactt tcaaacgtac
     2521 tccaaagtac cgtattgaga acaaatcaga taagctgatt gagcgcatga aatacaagct
     2581 gcctctagat aagactgtat tcattgagtt cttcatgggc ttctactgcg tcgcctgtat
     2641 ctacttctcc atcgtgaaga acaagccatt cgtagttcca ttcatgttga tctatgccgc
     2701 cggcttcttc tatgtggctc tctcctctct ctccgagtct ttcgttcgcg tgaagcgtcc
     2761 tgcgatcgac tcggcagctg atgctagagc ctgatcgctg gcctacttta gaattttccc
     2821 ttacgggaat gtctctcctc tcgggaggag agtgctttgt ttctcctttt ccatgtagct
     2881 ttccatcttc tcgcgtaaat ttgctcgagc aagcagagct ggatctatgc agaggccgca
     2941 aagtggactc tctgaactgg ctcgtaccaa acggagcgga atccttcttt gactttgtat
     3001 ttgagcaggc acagaagggt cttcgcgttc gaggcgattc tgcagcgcct atcgaagcaa
     3061 ttcactccgg cggtaaatcc tgggcggaga ggtttcctat tctccatgta tctgagcatt
     3121 ccacaattcc atcacgcgaa agtgcgcggc ccgtcagtcg tttcatctat ttgaacccgg
     3181 aaggggaggc actgcccttc gcgcggcgcg aagcagaatc gttttgtaat ctgttcccgg
     3241 atgcccgggc agcctttcgc actctggggg aaggtgaatt caacgaggcc atggcagatg
     3301 ctacgcatat ttactacttt ggacatgcca tgatggtgga tggaagtccc gcgatacccg
     3361 cccgggatgc ctggattcct ttgttgagcc ggagcatggg agacgtatct cgcaagaccg
     3421 tgtttttggg tgcctgtctc cgccaggctg gcatgactct tcgcattcct gggggtagat
     3481 tcatctatcc aatcacaagg attgcagatc gccctaactc cttcctcgag cgaattgccg
     3541 ggtccagtca tcttttgcag ggcttccatt ctgcctgcat tcgcgacgca gaggaagggg
     3601 acattcgtcg ttttgtttac cgcgtgaaag gctccatttg gctttgacaa atcaggcgca
     3661 tgtcgcaggt ttcggtggcc ggggggaatt tatggatgca gccgtgcgcc tcagagcgcc
     3721 caaccagcag aagagaaaga aggccagagc gcaggtcaac ctgcctgcgg actttcaaat
     3781 cctgggacat cacgaattcc tgccatgttc ccttgcggat attggatctg gcggcctggc
     3841 tctgatcact cgttcttctc tttacattgg agataagttg aaggtgcgat tcaagatcgc
     3901 cgagtctccg tttgaagggc agtgtgtggt cgtgcgagtc gtaggaaagg cagtgggagt
     3961 gcagttcgag aatctcagcc cggcagacga agacaggctg caggacttta tccacaaggt
     4021 cttcctggag agagaacgca aaaagccttg accctgaggc ttcctcgcga aagctggcct
     4081 ttgaagaagg caggcatccg tggaaaaagt ctactgcgcc aattgcgcac attgcatcgt
     4141 tgtaagacag tatgaagccg agcaggataa gtacatcctc cgtgttcgtt gcgacaagaa
     4201 gaagtggtcc aagcgttccg gcgaagaaaa gctctacaag tatttcacag ttgcccgccg
     4261 tatgcagcca aactgcgagt attacagaga catgggagac ctgcttccat acatcaagaa
     4321 cctcaaaaag gaactcccga tcaaagacga gatctactct gtcaaaaagt ccgctcgtcc
     4381 ttgagttcgc acctatggtg tgaccatgga actgaaagac atcataccgc cagaccgcat
     4441 cttttacata gagcggtctt ttaaatcaaa agaagaacta ctctcgcacc ttcttgagca
     4501 gctgctcaag aaggatggac tcgaagcgca caaagaccag gtattgaaag tactcctgga
     4561 aagagagtct tctatgtcca cgggcatagg ccagggcatc gccattcctc attgttccac
     4621 tgacttcgaa aaagatctgt ctgcagttct agcagttctc aagaagccaa tcgagtttca
     4681 agcagtggat gagcagcctg ttcaaatcgt aattcttctg gttctgccta aagatagatt
     4741 cgagaagcac atcaagacgc ttgcggcgat tgcccgtcag ttcaatgatt ctgtgtttcg
     4801 taagaaagtg gcgggtgcgg cttctgtaga cgaagtgcag gctgccgttg cggctgcaac
     4861 ggcatcagct taaaagttag atcctgtcaa tcactccatc gatcataccg tactcagttg
     4921 cttccttcgc agtgaggtag tagtcgcggt ctgtatcttc ttctacttgc tcaagagctt
     4981 tgcccgttgc gtttgcaatg atgcggttga tttcatcgcg agtcttcttg atttcttctg
     5041 cgtgaatctt gatgtcgatg gccggcgcta caatttggcc tgaaatgaga ggctggtgaa
     5101 tcatcactct ggcgtgtgga aaaccaaacc gtttgccttt ctttcctgcg cagaggagga
     5161 gtgcgcccat acttgctgcc agacccatac agatcgtgga aacatcggaa gtgatcatat
     5221 tcattgtatc tagaattgcc attccagagg tgatcactcc ccccgggcta ttgatgtaga
     5281 attgaatgtc tttgcctggt gcctgggatt caagatagag caggcgctct acaatgtggc
     5341 gcgctgattc atcttctact gcgccccaga ggaagatttt gcgctgatcg agaaatctct
     5401 tgtccagttc gttttgaagg cctgcaaatt cttccatagg atttttgttc ataagttcag
     5461 aggtggttcc tggtgtgttg tgtcaaaagc tttttcgtcc aacaggcagc atgcgaagga
     5521 gcaccatgcc agaggcactg agcagtgaca tcacaagaaa gaaaacggtt tcaggatcgg
     5581 caagtgttcg ttccatcttc gcggaaaaca ttgcgggatc tgactgagtg tttgtttcga
     5641 aggtgagaat ggtagctccg ggcgccgagc ttcttgcggc cggagtttct tctatcttcg
     5701 catactcctc ttctagcgtt ctgttttcgg tttctaattc ttcgatttgg cctcggattg
     5761 cgtgaaggcg gtcctgcagt ttctggcggg tgaaatatcc tgcttcccca aaaagaaaga
     5821 cgtgaagcag gaatgtgatg actaccatcc cgcccagggc ggccagctgg accttgagtc
     5881 tgctcatgga atcaagatcg gctgaccggg cgggttgctg aactagactg cgtttcccgg
     5941 gatgctgaag aatgccttct tcccggggta gttggcctgt ttttcgaggt gttcttcaat
     6001 gcgtagaagc tcgttgtact tggccactct atctgtgcgg ctgagcgatc cagtcttgat
     6061 ctgggacgcc cccgtggcca ccgcaatgtg cgaaatggtc gcatcctctg tctcaccgga
     6121 ccggtggctg atgatggcag tgtagcctgc gcggcgagcc atgtctatgg cagcaagtgt
     6181 ctctgtgaga cttccaatct ggtttacttt taccagaatc gagttcgcaa ttccttcgcg
     6241 gatggcccgg gagagctttt ccgtattcgt aacgagaaga tcgtctccca cgagttgaac
     6301 tttcttgccc agcttttgag tgagtttgct ccagccggcc caatcgtttt cgtccagacc
     6361 gtcttcgatt gtgatgagag gatactttcc gattaaggat tcgtagtact caatcagctg
     6421 gtccgggctt ttttctttct gcgcttccgc ttcgagtatg tatttgccgt ctttgcagaa
     6481 ttcggaggca gcgcagtcga gaccgaggaa cacattcact cctggtttgt agcctgcctt
     6541 ttcaatcgca gtcatgatca ggtccatggc gtcctggttg gatgcgacca tgggtgcgaa
     6601 tccaccttcg tctccgaccg aagtggcgag ccctttgtcg tgcagcactt tcttgagtgc
     6661 atgaaagatt tccgcgccgg ccctcagagc ctccttgaaa gaaggaaatt cgcgcggaat
     6721 gatcatgaat tcctgaaaat cagcattgtt gtctgcgtgc gcgccgccat tgatgatgtt
     6781 catcatgggg actggcagag tggaaacgct tggcccaccg agataccgat agagtggaat
     6841 cttaaggttt gttgctgccg ctttggccgc tgccagagat actcccagaa ttgcgtttgc
     6901 tcccagtctc cctttgttgg gagttccgtc caggtcgatc atggtgcgat cgagtgccgc
     6961 ctgttcaaaa accacacggc ctttgagggc aggcgcgatt tctttatgaa tgttgtctac
     7021 ggctctggat acgcctttgc caagatagcg attggaatca ccgtcgcgca attctactgc
     7081 ttcgtattgg cctgtggacg ctccggacgg cacagcggcg cggccaagag tgccatctgc
     7141 gagaatgacg tctacttcga ccgtggggtt gccccgggaa tcaaggattt cccgtgcatg
     7201 gatggtatgg atggttgcct ggctttcttt gcttacgatt tgtttcacaa tcaaatggga
     7261 agagcccgcg cgcgccggta aagcattctt cgtaaaaaaa atcaatcatg cgaatggctt
     7321 tcaatttttt tgattgaaaa cctgccgctg gctcgtccag tgcaatggtg tccgcgcagc
     7381 tcacactgga atcactcaac accgtagaaa caagcgttga gacatggatt gatttttgta
     7441 cagtcagtct acaatctgat ttttatcgcc tcactctcca gaaaacagga agcattgaaa
     7501 gagccgcggc ccttacactt ctcaataatt acatcagcac gttttctccg agcgaaagag
     7561 agcgtcttga gcgagacgct gagttcttct acggttacgc gcgcggattt attgaagagc
     7621 tggctccttt tcgctacaac aaaagcggct acgatcctgg agttcgttct gcgttcatgt
     7681 ccaagatcaa ggctctttta cgcgcgcaga aaaatccgga tggatcttac aaagacaaag
     7741 aaagatacat tttcgttgaa acaattgttc gaatttgctc atctctcgac ttcatcatca
     7801 aagtgcacga tgactacaaa gagtttctct tccgagagct tccacagttt cgcacagctc
     7861 ccaatctgaa ataatcttcg cgccgcgcct gcgattccag cagctttaga taattctgaa
     7921 agacttcagt gagttgtaca tcacttcgat agtcaccagc aaaatgcctg ccagaggaat
     7981 ggcgatgatc attccaacca ctccaaacaa tcgttcgcaa gtgatgagtg cgaggatcgc
     8041 aatcaatgga tgtagttctg cgcttcgcgc gagtatgacc ggctgagtaa aaacgttgtc
     8101 gaacaggtgg gcgcaggcaa atacaacgag caccagcatc acaacgtttc caccctgctg
     8161 cagagcgatg atgagtgcgg gtatgattcc gatgaccggt ccagcgtacg ggatgaaatt
     8221 gagcgaaccg gccaccacgc ccatggtgaa tccgtaatcc agtccaatcc acataaatcc
     8281 aacggagaaa atagaaacgt tgatcaagag ctgcaccgcg agtccgcgca aatagccggt
     8341 gatttgcttc tttactctgt gcgctacaag caggctcatt tcaaagtaac gattcgggac
     8401 gatccgaatg atgcgaagat agatttcctc tccctggaga aggaagatga aagtgatgat
     8461 gggcgtgatg atcaggtttg taacaatgct gccgaggttt tctttcatgg tctggccctg
     8521 ttgcttagac cattcttcta tgaatcctgt gatggtatcc atgcgcagcc gctcgattcg
     8581 aatgaatcta ggcagttttt ctgcaagcat tgcgtttgtg tcagtgaaaa acacattcgc
     8641 cttcttttga atgtcaggcc agcgcgaaac aatgtcgcgc acctggtctt gaatgagcgg
     8701 tactatgtta taaacggtaa agtaaagagc ggcgatgaag agcaaaaaga cgatcacggt
     8761 tgcaagtatg cgtggtagtc cggctccttc gagtagatcc accagaggat tgagcatcag
     8821 cgtaatcaga aatgcaatgg caatggggac actgacttct tttgcaccgt agagcgtgat
     8881 gaaaagaaag aagatggtga tggcaaaaaa cagaagctgc acgatgcgcg cagacaggtc
     8941 aagggaatcc tgttgcttct gctcttccat tacttatcct gccagatttt ctttctcagt
     9001 ttctggttgg agttttcgct catttgagaa tctagcttct cgagttccat gtttgcatga
     9061 atcagtcttt gtccaatgag ctttgccatg ttcagcataa cctgaccgcc cagtctaggc
     9121 ttgcggtcca gaatgctcat gaggtctggt ctgaaaaatc caatgattcg cgcgtggttc
     9181 tttgccgcag cagatgcagt gcgaggtagt tcttccagta gtgcgagttc gccaaaaaaa
     9241 tctcccgatt cgagagtggc cagatgtttc tcgcgtttgc catcgcggtg atagatgtca
     9301 atgactccat ccagaatcac gtacattcca acgccaggtt ctccttcgcg aaagatgtgt
     9361 tcgcccgccg cgtaatcgcg cagatggcag aggcttcgaa tttctttgag acctctgtcc
     9421 gagatatctc tgaacatggg tgcgctcttc aggcgcactt ccactgcgtc ttccgctgaa
     9481 aaggggctca atctggacca gaaaggggac atctagaata aagatcggtc ctcgtagatt
     9541 catgtcggat gcggaatgtt gtttttttgt ggactccaga caaaattcta aaaactcgtc
     9601 cataaacctc acgggatgta gcgcaggggt agcgcatctg ctttgggagc agagggtcgt
     9661 tggttcaaat ccaatcatcc cgattgtagg tgccggtagc tcagttggat agagcaactg
     9721 ccttctaagc agtgggtcgg gggttcaact ccctcccggc gcaccagata cggcggatgt
     9781 agctcagttg gtagagcatc agattgtggc tctgagggtc gcgggttcga gccccgtcat
     9841 tcgcccatcc ctttcatcgc ttcgcgcgct ttcctttttt ctttttgcga agatagacca
     9901 ttccgaatcg ctatcgcttg acactctgac tgcccgccag ttttatgtcc gccagaccgg
     9961 aatctgaggc gagcgtggtg gaattggtag acacgccaga cttaggatct ggtgccgcaa
    10021 ggtgtggggg ttcaagtccc ttcgctcgca gaaaaaacgc gggaatagct cagcggtaga
    10081 gcacctcctt gccaaggagg gggtcgcggg ttcaaatccc gtttcccgct tatttttaca
    10141 aacaagattg aagaggcgcg accctggaat acaagacaaa acgccttgaa aacgcatccg
    10201 tgcagattgt tctgaagaac acgccggagg aagtgcagca ggcttacgcc actgcatatg
    10261 aaaaagctgc aaagaaagtc cgcgtgcccg gattcagacc gggcaaagtt ccaatggaag
    10321 ttctggaacg tgagctgggc gatgctgttg caggagacgc ggcgcagatt ctggttgcga
    10381 gtagcatgga aaagctactc ggggaaatca aacctcctcc gatcagtgtt ccttcttttg
    10441 aagtagagca gttcgatcgt aagcagggag ccacataccg aggtacgttt gatacgtatc
    10501 cggaagtgaa gctgggcaag tacaagaaga tcaaagcatc tcaagatacc gtggaagtcc
    10561 tcgaagagga catcatggcg gaaatagaga agcttcgcaa agatcattcg atcatgcaca
    10621 caagagacga tggcgctcta gccggggatg ttttgaccat cgagctggac atccgcgaat
    10681 ctggcggaaa aaagaaactc tatcataaca aagactacaa gatggttctt tcagaacctt
    10741 ctccttttcc tggccttgtg gaacaactgc tgggtgcaaa atctggagaa tcaaaatcat
    10801 acgaccagca gatggaagaa agttttcctg atccaaagtt tgcaggaaag ctggtgtctg
    10861 ttaccgcgaa catttctact gttcaatatc cagtgttgcc tgaactcaat gacgatctgg
    10921 caaaagaagt gagtgagttt gatactctcg gtgcgctaaa aaacaatatc cgcgaaaccc
    10981 tgacgaagca agcagacaac atactcaaag atcgttgcat gaagacaatc ctcgacctca
    11041 ttgcgaagga ttcgaagacg gaaatgcctc agaccctggt ggacgctgaa atcgaagaaa
    11101 gagtcaagca gatcgcgcgc agcattggcc gcaaagacct cactctggag caactcgcgg
    11161 aaatgatggg caagcctgca ccagaactca gaaaagaact caacgaagcc tctgttggat
    11221 ctgtgaagaa acggctggtt ctggaagaga tcgcaaagaa agaagaagtc aaagtagaag
    11281 aagctgatat actggagtat gcagaagagc gattcggcac cctgacagat gacctcaaga
    11341 agaccttatt gtccaatgag aagtttctcg atgaaattga gggggctgtc ctcttccgta
    11401 agacgtttga ctggattcac cgaaatgccg acattaaacc aggcgagaaa gtgaagctgt
    11461 ctaccctgat gcaggcaacc ggcaccggcg cttgacatcc aggacgtttc tgatttccct
    11521 gatccaactg agaggtatct tccatggcct ttatgcccat tatcagcgag caaaccccta
    11581 gaggggagcg tcaatacgac gtatattccc gcttgctcaa agacaggatc gtctttctag
    11641 gctatcccat cgacgacgtg tatgcgaaca tcgtgattgc ccagcttctg tttctggaag
    11701 cggaagatcc tgaaaaagac gtgtatgttt acatcaattc accgggcgga tacatcacga
    11761 gcggtcttgc ggtatatgac accatgcaat acattcgccc gaatattcgc accatctgca
    11821 ttggccaggc ttccagcatc gcctctctgc tcctcgcggc cggtgctccg ggcaagagat
    11881 ctgcccttcc aaattcacgc atcatgatgc accagccgct cggtggagtg acagggcagt
    11941 cgcgcgacat tgaaatccag gctcgcgaaa tcttatccgt aaaggataaa ttgaatgaaa
    12001 tctacgcgaa gcataccgga aaaccgcttg agcaaatcga aaaagacaca gatcgcgttc
    12061 atcacatgtc cgctaccgaa gcaaagaact atgggctcat tgacgcggtg atcgaatcgc
    12121 ccatcaagct agaaaaaaaa tagaaaggaa tgcttgccga tcccggcagg ctcctgagaa
    12181 tacaaggcat gaagaagaag acgacggatc gggaaaaact tcactgttcg ttttgcggaa
    12241 aggaacagga ttccgtgaag cggctcgtgg ccgggccggg cgtctacatc tgcgatgagt
    12301 gcattgagct gtgcaatgag atcattgcgg aggaaacaga tacagaacgt ccagtaggga
    12361 ctgcaaccgg tgcggaactt ccgcgcccgg tagaaatcaa gaaaatcctg gaccagtacg
    12421 tgattggtca ggacagcgcg aagaaggcgc tggcagttgc cgtctacaat cattacaagc
    12481 gcattgagca gaatactcgc aaatcagatg tggatctgga caaatccaat attctgctga
    12541 ttggtccaac aggttcgggc aaaactctcc tggctcaaac tctcgcaaaa atgctcaagg
    12601 ttccgtttgc catcgtggat gctactgctc tgacagaagc aggctacgta ggcgaagatg
    12661 tggagaacat ccttcttcgc ttgattcaga acgctgagaa tgatgtgaag cgcgctgaaa
    12721 ttggaatcat ctatattgat gaaatagaca agatctctcg caaaggagac aacccgtcta
    12781 tcacgcgcga tgtttcggga gaaggggttc agcaagccct cctcaagatc ctggaaggca
    12841 ctgtggccaa cgttcctcct caaggaggac gcaagcatcc tcatcaagac ttcatcactc
    12901 tggacactcg caatattctt tttatctgcg gcggtgcatt tgttggtctg gacaagatca
    12961 ttgaacgcag gctcggaagc agaacgattg gtttcaatcg ggacgaagaa gcagcgcgca
    13021 aagcaggaga gaaaagcgaa atcctggcca atgttcttcc agatgatctt atgaaattcg
    13081 gtttgatccc ggagttcgtg ggtcgtttac cgattgtggc cactcttcag gatctggatg
    13141 tagaaactct gaagaaaatc ttcatggagc cgcgcaattc tctcctcaag cagtatcaga
    13201 agatttttga aatcgaaggc gtgaaactta aattcacaga cgaggccatc actcgcattg
    13261 cggagcttgc catcaagaga gaggcgggag ctcgtggact gcgatccatt gtagaacacc
    13321 agatgatgga tttgatgtat gatattcctt cgcgcgatga cgtggagagt gtcgccatca
    13381 ctcctggagt gattgagtcc ggagactcgc ccgtgctcag tttcaagaaa gagggagcga
    13441 aagaaaagga aaaggcatcc tcttctaaga aacaagagaa gctcgcctga cgtctgtcag
    13501 gcttctgctg catctcgcgc cgctgctttc tctctgtttc tctctatcat gtagacgatc
    13561 gccactgcaa tggcatagaa aacgcaaaga ggtagagcta caacaaagag agacacaggg
    13621 tctcccggtg tcacggccat cgatagaaga aacataccaa caattacaaa tcgccatgcg
    13681 cggcgatgaa ggctgatggg aacgactccg agagctccta gaattacgat gagtagaggg
    13741 agctggaatg ttactcccgt gcctatgtgc agaataaaaa agaagctgta gtatttatcc
    13801 agactgatca gcgcctgtgt attgggcggc agcaaaaatt tgaacatcat ctccacagaa
    13861 agtggaaaga tgtagaacca gcagaagagc actccgcccc agaaaagcag gcaggaaaaa
    13921 accatgctta tccgtgcgta gatcgctgtt cgccgttcca tcgcaggtgt tacaaattca
    13981 aagagcatga aaatgacgac aggcaagccc gcagcaaatc caacagccac gctcaactgt
    14041 atcatcgtgt tcaaagcgtc catggcactt ccgagcatca tgttgagtcc ggtggcgcga
    14101 tggtacggtt cagaaatata gcggtgaatg tccgaaatga aaaagccggc agcaatagaa
    14161 aagatagcaa ggatgcccag agaccagagg attcttcttc tgagttcttc caggtgatcg
    14221 cccagagtca tcacgccttc gcgtggatcc ggcagttctt ctggcgcagg cgctggcgtg
    14281 ggaggtggct ggatcggggc gcgggccctc gtggccctct tgcgtcctgc catcaggatc
    14341 ttgctctaga cttctttgct ggctttcgag ctgcgggctt tgcagccgcc cttttccggg
    14401 gtgctgcagg cggaggagtg tatgtttccg ccggttgtgg ctggtgctcg atcatctgag
    14461 tggtttgtgt ctggtttgga ttcaggagag cctgacgaaa ttcacgaatg ccgccaccca
    14521 gatcttttgc cagagccggg attttgcggc caccgaataa gatgagtaca atgactagaa
    14581 tgatgaccca gtcgaagggg ctgttcgtta aaaaaagtac catatgcctg cctgggtcca
    14641 aacaatccat gaatcgcgcg aaaacaagtg attttcactt gcatttcctt ttttgcgggt
    14701 aggataagac cgaagtttaa accagatggg cgtcaccaaa gagcagaaga acttatacaa
    14761 cgaacgggtg aaatcctata aacagggtct cgacgagatc aagaaggaaa tggccatggt
    14821 gaaaggaatg ggaaaatctc ccactctcca gccctatgca accattcgcc tggccatcga
    14881 gagtctgaag aacgcgacca tccatgcgca gatgtctcgc ctttcgtatc gcatcctgaa
    14941 aatccgccgg gatgaagtgc tcgcggatgc gaagaaagat atattcaaca gcctcacaga
    15001 tttgctcaag ctcgtgggtt cggatgtgga cgaaagtcta acggagaaca aagagaggct
    15061 accgaagatc gctgcgatga cgcccaggca gaagctggaa ttgctgaaag gatttcgctc
    15121 tgtgacggac tatgtccagg aggcgatggg cccgagtcac aagatgagat ggtcttttcc
    15181 tgaattgcac tacaagattg cggtgcttgg aaaaaacatc atggatttca aggaatacga
    15241 gagatctaaa gatccaacgg aagagcacta ccaggacagg cgggcgctct tgcagttcat
    15301 agtggatcaa tgccatttcg cggcacagga atatcgttcc aagcatgaac tcagcacgaa
    15361 agaagtaacc gatttgcaga gcgtgcgaaa gatgtttgag ctgatcaaaa agattcagag
    15421 catgaccgga aacaggacgg aactcgagaa gataacaact tctctagatt ccgtaaacga
    15481 aaaaattgaa gcgatgatgg ccgagaaaaa ggataaaaag aagaaaattt agtttccgcg
    15541 ccccttccgc cccccgttta tggaatcagt cttgtaggag gacccatggc agttgtagca
    15601 ctcaaagatg attcttttag ccaggatacg aaagagggcc ttgtcctcgt tgatttctgg
    15661 gcagagtggt gcggcccctg tcgcatggtc gcccctattg tagaagaact ttccagcgaa
    15721 atgcctcaag tgaagtttgc gaaggtgaac gtagatgaaa atcagcgcat cgcgcaaact
    15781 ctgggcatca cagccatccc aacactcgtt ctctataagg acgggcagcc ggtggatcgt
    15841 gtcgttggtt tgcttccaaa accacagcta aagaacttca tcaataagca cgtctagtct
    15901 accgttcaga ggtcctgctt gcgcaggacc actgtgtgag tatgcgtgtt cgttgaagtg
    15961 ctctccacag tcaccggaaa tccaagttct atcagagcag cctgttccag agtcagcgtg
    16021 aatgcgtggc tgtgtccctg gttttcaaga agggcgaacg ttcgcattcc ggaatgaagc
    16081 agcctgtatg taacattggt tccgtgatca tggccggcgc tcatgcttac gatggcagca
    16141 atgcgaggat cccggct
//
LOCUS       DAORFF010000012        15032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1683_length_15032_cov_4.103225, whole genome shotgun sequence.
ACCESSION   DAORFF010000012 DAORFF010000000
VERSION     DAORFF010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 15032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15032
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1683_length_15032_cov_4.103225"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /gene="queC"
                     /locus_tag="PKK76_01090"
     CDS             <1..627
                     /gene="queC"
                     /locus_tag="PKK76_01090"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNL67081.1"
                     /translation="ATVLQIAKSHGYSLIALSFDYGQRHKFELERARELAIRANAKHL
                     VLRIDQELFKNTALVPGGPAVPEKRQIDDSIPVTYVPARNILFLSHALAVCESNDIGN
                     IFIGVNALDYSGYPDCRPEFIQAFEQMAALGMKRGVEGRPIIIHAPLMQWNKARIIQE
                     GMKLGVDYSRTSSCYQPSASGKPCGLCDSCVLREKGFAEAGLIDPLKS"
     gene            665..1405
                     /gene="kdsB"
                     /locus_tag="PKK76_01095"
     CDS             665..1405
                     /gene="kdsB"
                     /locus_tag="PKK76_01095"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNL67082.1"
                     /translation="MKVLGVIPARYGSQRFPGKVIAPLGKKPMVQWVYEAASRCKDLD
                     HLVIATDDERVRDAVKAFGGEVLMTSEDHKAGSDRLAEVSESYKDYQIVVNIQGDEPG
                     IEPDLISGVVQLLKDHPEWEVTTAARPFEDAEDPLIPNRVKVTLTKKGKVLYFSRSLI
                     PFPRNKTGHPVFLHLGIYAYRRDFLLRFRTLPGSGLEDTESLEQLRVLENDYSMGAHI
                     VKGSLPGVDTPEDLQNITAIFRKKGMLE"
     gene            1423..2322
                     /locus_tag="PKK76_01100"
     CDS             1423..2322
                     /locus_tag="PKK76_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67083.1"
                     /translation="MEIVRRIRTLNEEGKRTSIPFSDTIYRKLLSDLCESEDRMRFYL
                     RVLSDAHYLFTIRLVEPDERLLIHGLEAYVVCDVPVIMRLKEVSYSELELSYEGQYYK
                     RKQATLILRELIGNARQFNNTPLGKSMNLAMMLQQYEQFMATRFAEFSEGWKQKKLNE
                     LLPEVGFDIGSGSATASSAKTEAQPRRAIDSEHLQKIEEMNRSGKWGEAVDKFGVQFL
                     VRIHLRKYEFEQIKTLIKQHKIATEKDLRFLRDTLKMMEGRFEEDPELVKHKEEMIEL
                     RRLAQLKMNQVLIARKEFESQGN"
     gene            complement(2398..3546)
                     /locus_tag="PKK76_01105"
     CDS             complement(2398..3546)
                     /locus_tag="PKK76_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67084.1"
                     /translation="MKRRLAFLLLLCLPVAIHAESVAEKATEKRNELIRYLREIRTMA
                     YNYPCDPFPDCQAALPQGDDKKAPATPAGTTAQPERIKLFGDIKRIYQEGMVYLYEGN
                     FVNSYNRFLDSQARVERMLEGLSQLYLDRAEAMMRDSIEKKDPTDPADMTATDISIDY
                     GQKSDKRRDFARNREIPRDSRTYDQKEVHYAHNKYEIERNIEMGYKHLGLAREARRRA
                     LIVDTNLAPNQRMEPHHRRARIEYYLDCIRMARLAKLNGERIYQLKYPYDNYALISPF
                     GKTEIVRGDHPKEAKFPVIAGKERDWSTDENTADANKKADKSNPYIYPANLNPVFDLR
                     IPEKYRRDSSDARDQIYADQIDINVDFRYIPEKRNKPTADSTTPAKQP"
     gene            3663..4571
                     /locus_tag="PKK76_01110"
     CDS             3663..4571
                     /locus_tag="PKK76_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HNL67085.1"
                     /translation="MRIKQPEISKAKAISYRVKDKSTCKVCGFEFNREQLHSGGGRLI
                     AGKLTQELRRLYEVSKRYGKVYPLAYSILTCPQCLFSSFPTDFALLPPDEVEKLKKAT
                     LDRRTSVEKIVGPVDFADDRNLVSGAASYLLAIDCYQMRGMGIAPTPKKAICALRGAW
                     LFQDMAEEFPKYGFEKLSEFLYLKSVSWYIPTLEIMTNGREPHEQFINLLGPDTDNNW
                     SFDGVIYINGYLTRKYLNQLAPTKEKKLEYLDRSKRYLGKLYGMGRASKSKPSVIIDM
                     AKELYEELQKEIDTINGVPPTAPAAS"
     gene            4591..5442
                     /gene="truA"
                     /locus_tag="PKK76_01115"
     CDS             4591..5442
                     /gene="truA"
                     /locus_tag="PKK76_01115"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNL67086.1"
                     /translation="MVSYDGTKYAGFQYQKSGTSIQELLENALQIVVREHTRVDCAGR
                     TDSGVHATGQVATFLSPVAPDPGKLTYSLNSLLPFDVSVQKSLNMPRNFHPRFSCIAR
                     EYEYMYHIGARSPFWRGRSWHRKKGFEIEEINQELAAICGHRDFIALTKTLYEGKGTV
                     RYLDSAVLYRKEDPWTGHGDMIVLRVRANAFLHNMIRILAGTIVDRAAGKLSSITDAV
                     LSGSRFKTGQTAPSEGLYFRHAFYVPGTAPGLKELALPDDHPLMRIHAGRMRSSREGD
                     QENHAKE"
     gene            5429..6067
                     /locus_tag="PKK76_01120"
     CDS             5429..6067
                     /locus_tag="PKK76_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67087.1"
                     /translation="MRKSRILICIVLLAAGTAASSLHAQEQQSASRVKHEIGLWIGAS
                     NPLPGTELDAALDSNLGGGLFYRMTWPWILYTEFGTSYSNYFSRETQSATVIPIYAAL
                     SYPIQLPIRMQVFLKAGGGTAYVLVRPINRHGWEPLGYLGTEFSILAGRRFRIGLRID
                     YNLVYEKHLKDPEQPLLPITTGTTDPRYQKTQYFKKRNGHFFHFGLMTSFIL"
     gene            6075..7310
                     /locus_tag="PKK76_01125"
     CDS             6075..7310
                     /locus_tag="PKK76_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67088.1"
                     /translation="MKRCRLTLLLIALLMVASCKGKNAEPLLSSLLGMNSKTVLILKG
                     TYASDSPLEFTELNNNNLYVDAEDTSVDLVGVPRYSQLPIYLDIGEIRLSSKDYGNGL
                     WSIDTAKDAKAFWDVIASERQVYCSQPYSVSLENDTCFSTGGLINYQEFMNGRGALYP
                     SRDVGPGVYLHAGIFVRAIATGHSKLAGAIQQTAFDNNVVINATNITSLVQYDPNLND
                     GLKQVLPPYWFPLHFNSIMGGVTANSMYMTNDYVTRVVEMRFNMKENMMVHGFTDTNG
                     YRSIVAFSDWRRAHAQERDLGGNVLMRARVYYPDYVNTLVIQGGTKSNRHYYAIVDPV
                     ETYIEDNLPVAATPVRQGTNIIKNLMGGVNYIIQCRFDANNDGYPETILDQQTFGVLP
                     GPGTVTIGCQCGGGVTLCH"
     gene            complement(7336..7911)
                     /locus_tag="PKK76_01130"
     CDS             complement(7336..7911)
                     /locus_tag="PKK76_01130"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNL67089.1"
                     /translation="MIKPLPSQTARDWHHPNCYGCGPDNAKGLHADFPFHNETGEVRF
                     QHSVEKSFEGAPGFVHGGVIAALLDEAQGTLCFHVGHFVMTEQLHMKYHRAMPLETQV
                     SVRCWLTAVRKRRLYTRGTIHSPSGELLASSSASWYVLTERILQKLFAERYSPEDHAR
                     NAAILEANRKRAREIRRRLRQVVSASNQAGF"
     gene            7912..8610
                     /locus_tag="PKK76_01135"
     CDS             7912..8610
                     /locus_tag="PKK76_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL67090.1"
                     /translation="MSDFRIDSRRASNLYHRVDLDIVSFAVAPLGCNCSILRETATNE
                     TIVVDPGGDADRIIDLLTEKGCKVKYIVHTHAHFDHVLGTHEVASHCRNSGHASAVCM
                     HPGDLPLYRILPQQCGWFGLPPVEAKEEISHRLEDDEVLSFGRFKMHVLHTPGHTPGS
                     CSFSIAERGILFSGDTLFAGSIGRTDLPGGDYDQIIKSIKDRLMVLDDGTTVIPGHGE
                     FTRIFDEKRTNPFL"
     gene            8617..9999
                     /locus_tag="PKK76_01140"
     CDS             8617..9999
                     /locus_tag="PKK76_01140"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNL67091.1"
                     /translation="MLFHTLDFALFFGLVLAAYHLAPTSFRKNLLLGASLFFYGYWNW
                     RILGLLAVTVTANYICALQLEKTRKRIWLIVAICVNLGLLGFFKYYGFFAESLSLLLG
                     RMNPGLPALNVLLPVGISFYTFELISYVVDVSRGLPAEKNFRDLLLFGTYFPKMMAGP
                     IARAGHLIPLLKDPPKSTLSGIQSGSILAFSGLFKKLVIADNLALEVNRLVGNHGAPM
                     SALDIGKHTISDAPTLILAATFFAVQIYADFSGYSDMARGMSRMLGIELVQNFNHPFR
                     ATTPAEFWRRWHITLGSFLRDYIYIPLGGNRSGMGMQMRNLLIVWTIGGLWHGASAGY
                     VAWGFYCGLCLCVYVLLRPFLSGGGPIVRGAGVLATFLTFALGLMIFRLGSPLEVARL
                     LSIWKAPEIHPLAFLFCLPVFVSHINGDNQSILEKLSPRAKWACLIAMFYVLIFLGRF
                     SGDEFFYFQF"
     gene            9974..10654
                     /locus_tag="PKK76_01145"
     CDS             9974..10654
                     /locus_tag="PKK76_01145"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNL67092.1"
                     /translation="MSSSTSSFDSEEMLKRLLGNSDRAPERERMVTEQIQRRGVKDPR
                     VLRAMRAIPRHAFIPGNLQSEAYEDHPVSIGEGQTISQPYIVAYMTELLEVKPGHSIL
                     EIGTGCGYQTAVLAILGAEVYTIEIIASLQQTARQTLHALGFENIDFYCGDGSSGWPQ
                     KRTFDRIIVTAAPPTIPQELVNQLAESGKMVIPVGEMQQDLTVMRRSNGRLSEEKVLP
                     VRFVPITH"
     gene            10738..11655
                     /locus_tag="PKK76_01150"
     CDS             10738..11655
                     /locus_tag="PKK76_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNL67093.1"
                     /translation="MGRSLVILAFLLCSSLSAEIVYMKDGRILEGSIVAQNDQTLDIK
                     TKNGLQKIQKSTVRRIAYHTAEEKAKLEAERKARRDAAMKELERMRQLRAQEEEARKR
                     ADIARQAELAKQRAAAAKAVRESVEREDMEKPDDIIGFKDFAWRSALVPGWGHFAIGR
                     PIVGSIYAGATAGALYNIYDKRRKALAARDANHNDVLVNLLVSVAPVDAPLPFRLAYG
                     LDANRRAFTEYRARINRYNYSIASLGIVYGVQMTHIILNGITWESGFIVDSGVRPTFD
                     ISYNEHNTDERSFNREPDKQIVASFRMTF"
     gene            11700..12926
                     /locus_tag="PKK76_01155"
     CDS             11700..12926
                     /locus_tag="PKK76_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL67094.1"
                     /translation="MAIPPLDISKKIVDFFEKATVGTLSGIRDILDTSMGSTANRLSE
                     LTDKPLVKFTEIQGAFQKAAGQLSTANQVTAFGLATAIQSVTDAFDSAGNALRIADEF
                     THKHIFENVHISSTAGTSFDALLKTKIVASFRLRDADCSAQQIAEAVKASGKTSAVLF
                     VPGLFCDDGMWTQGPEPLLPLIESMGYASVLIRLHPGLPISENGQKLVTLLDDLFANI
                     EIPLHIISFSNGGLILRSCLYYAKLAGKPWLKHLRKVAIISPPDGGSYIEKLGFWLGL
                     ALKSTPSLGLQLLGWVGHMRSDAMKDLSHGIIRQEDRHELSQIKRYGVDLYYNELDDL
                     DAYLIYSLISESNDPVKTWLGDGVCEKKSLEYLRESVYLKKPNPEKRVRVIAQRSHFQ
                     ILSSAELRMHLKEIFS"
     gene            12923..13264
                     /locus_tag="PKK76_01160"
     CDS             12923..13264
                     /locus_tag="PKK76_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNL67095.1"
                     /translation="MKDKTIFAKIIDREIPASIVYEDDQCLAFKDVHPQAPVHILIVP
                     KKPIDRLSSASGEDQLLLGHLLLTANKIAEQAGLKDFRLVVNSGPGAGQTVLHLHVHI
                     LGGREMEWPPG"
     gene            complement(13310..13624)
                     /locus_tag="PKK76_01165"
     CDS             complement(13310..13624)
                     /locus_tag="PKK76_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich protein"
                     /protein_id="HNL67096.1"
                     /translation="MRSILYSTLVVVLLALGFSQAIAQPATCEAACAKYRECAVDIWK
                     KNNKVPTAEQSKTMYGGCMRTCGKFKEKTLVCYKQTLAAKGDACMTYWSCVQSAYNSG
                     KK"
     gene            13855..>15032
                     /gene="glnA"
                     /locus_tag="PKK76_01170"
     CDS             13855..>15032
                     /gene="glnA"
                     /locus_tag="PKK76_01170"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNL67097.1"
                     /translation="MAKSPSEVLNWAKSEKVEFVDIRFTDMFGTMQHFTMPLHAIDEE
                     SFTYGIGFDGSSIRGWKAINESDMLARLDADTVFIDPFLEHKTMAVVCDIYEPGANPK
                     PYGRDPRYILKKAIKYMQDSGIADTVWFGPEAEFFIFNDVRFDQNMNSGFYFIDSDEA
                     SWNMGRDEGPNLGYKIRTKEGYFPLPPSDTFQNIRSEMLHEMEKAGIKTEKHHHEVAS
                     PGQAEINFVVDEGIKMADKLQLYKYIVKNVARRHGYSATFMPKPIFGDNGSGMHTHQS
                     LWKGGKNIFAGNKYAGISDQCLNYIGGILSHGPALLAFTNPTTNSYKRLVPGFEAPVN
                     LVYSARNRSASIRIPIAVSASEKAKRIEFRTPDASCCPYIAFAAMIMAGLDGIQSKID
                     P"
BASE COUNT         3899 a         3802 c         3723 g         3608 t
ORIGIN      
        1 gcaactgtat tgcagattgc gaaatcgcac ggatactcct tgatagcatt atcctttgac
       61 tacggacaac ggcataaatt cgaactggaa agagcgcggg agcttgccat cagggcgaac
      121 gcaaaacatt tagtactacg tattgatcaa gaactcttca aaaatacggc tctcgtgccg
      181 ggaggccctg ccgttcccga gaaacgtcaa atcgatgatt ctatccctgt cacttatgtg
      241 cccgcgcgaa acatactatt tctctctcac gctctcgcgg tatgcgaatc caatgacata
      301 gggaatattt tcatcggggt caacgcgctg gattattcag gataccctga ttgcagacca
      361 gaattcattc aggcattcga acaaatggca gcactgggaa tgaaacgggg tgtggaagga
      421 aggcccatca taatccacgc gccactcatg caatggaaca aggcgcggat cattcaagaa
      481 ggaatgaaac tgggagtaga ctactccagg acctctagct gttaccagcc ttccgcttct
      541 ggaaaaccgt gcggattgtg cgacagttgt gttttgcgcg aaaagggatt tgcggaggcg
      601 ggcctgatcg acccactgaa gagctgattg acgtaccaac cccctctcac agcctgaaat
      661 ttagatgaaa gtacttggcg tcattccagc gcgatacggt tctcagagat ttccgggtaa
      721 ggtcattgca ccgctcggca aaaagccaat ggttcagtgg gtctacgaag ccgcatcgcg
      781 gtgcaaggat cttgatcatc ttgtcatcgc aacagacgat gaaagagtgc gggatgcagt
      841 gaaggcgttt ggcggagaag tgctgatgac atctgaagac cacaaggcgg gctcagacag
      901 actcgcggaa gtttcggaat catacaaaga ctatcagatt gtcgttaaca ttcagggaga
      961 cgaaccgggc atcgaaccag acctgatctc tggagtcgtt cagctcttaa aagaccatcc
     1021 tgaatgggaa gtaactacag ctgctcgccc ctttgaagat gcggaagatc cgctcattcc
     1081 aaaccgcgtc aaggtaacac tcaccaagaa aggtaaagtg ctctattttt caagatcgct
     1141 cattccattt ccaagaaaca aaacgggaca tcctgttttt ctccatctgg gaatctacgc
     1201 gtatcgtcgg gattttctgc ttcgctttcg cacgctgccc ggttctggtc tggaagatac
     1261 agaatctctc gagcagctcc gagtacttga gaacgattac tcgatgggcg cacacattgt
     1321 gaaaggttcg ctacctggag tggatacacc cgaagatctg cagaacatca ccgccatctt
     1381 tcgcaagaag ggaatgttag agtgaccctt ggcgaacgcc tcatggagat cgtccgaaga
     1441 attcggactt taaacgagga aggaaagcgg acatcgattc cgttttcaga cacaatctac
     1501 cgaaagctgc tgagcgattt gtgcgaaagc gaagatcgca tgagattcta tcttcgtgtg
     1561 ctttcagatg cgcactacct tttcacaatc agactcgtgg aaccagatga gcgacttctc
     1621 attcatggac tagaagcata cgttgtctgc gatgttccag tcatcatgcg attgaaggaa
     1681 gtatcttact cggaactgga gctctcctac gaaggccagt attacaaaag aaaacaggca
     1741 acgttgattc tgagggaact gattggcaac gctcgccagt tcaacaatac ccctcttgga
     1801 aaaagcatga acctggccat gatgctccag caatacgagc agttcatggc aactcgattc
     1861 gcagaattct cggaaggctg gaaacagaag aaactgaacg aactacttcc cgaagtgggc
     1921 tttgatattg gatcaggcag tgcaactgcc tcgtccgcaa aaacggaagc acaaccacgt
     1981 cgagcgattg actcggaaca tctgcaaaaa atcgaagaga tgaaccgctc tggaaagtgg
     2041 ggagaagcgg tggataagtt cggtgttcaa tttctggttc gcatccacct tcgcaaatac
     2101 gaattcgagc aaatcaaaac gctgatcaaa cagcacaaaa tcgctacaga aaaagatctt
     2161 cgattcctga gagatacact gaaaatgatg gaaggtagat ttgaagaaga tcccgaactt
     2221 gttaaacaca aagaagaaat gatcgaactg cgtcgactgg ctcaactgaa aatgaaccag
     2281 gtgctgattg cgcgcaaaga gttcgaatcg cagggaaatt aaaaaagccc cgccatggcg
     2341 gggcttctca atcgaatgaa tgatgcgctg ccgttcacac gggcagcgca atccaaatca
     2401 aggttgtttg gccggcgtgg ttgagtccgc agtaggcttg ttcctctttt caggaatgta
     2461 gcggaaatca acattgatat ctatttgatc tgcatagatt tgatcgcgcg catcagacga
     2521 atcgcgacga tacttctctg gaatgcgaag atcaaataca gggttcaaat tcgcaggata
     2581 gatgtaagga ttggacttgt ctgctttctt gtttgcgtct gcagtgtttt catctgtaga
     2641 ccagtctctc tctttacccg caatgacagg gaacttcgct tctttaggat gatcccctct
     2701 cacgatttct gtttttccga aagggctaat caatgcatag ttgtcgtacg gatacttcaa
     2761 ctgatagata cgttctccgt tcagtttcgc aagacgagcc atccgaatgc aatccaggta
     2821 gtattcgatt cgtgcacgac gatgatgcgg ttccatgcgt tgattgggag ctagattggt
     2881 atccacgatc agagcgcgac ggcgtgcttc gcgtgcgagg cccaggtgct tgtatcccat
     2941 ttcaatattg cgttcgatct cgtatttgtt atgggcgtag tgcacttctt tttgatcata
     3001 cgttctcgaa tcgcgaggaa tttcacgatt gcgcgcgaaa tcgcgacgct tatctgactt
     3061 ctgaccgtag tcaatggaaa tgtcagtcgc agtcatgtct gcgggatccg ttggatcttt
     3121 cttttctata gaatcacgca tcattgcttc cgcacgatcc aggtagagct ggctaagacc
     3181 ttcgagcatt ctctccacgc gagcctgaga atccagaaac cgattgtatg aattcacaaa
     3241 attgccctca tacaggtaga ccatgccttc ctgatagatg cgcttgatat ctccaaagag
     3301 cttgattctc tctggctgcg cagtggttcc tgcaggtgtt gccggagcct tcttgtcatc
     3361 cccctgagga agggcggcct ggcaatccgg gaatggatcg cagggatagt tgtaagccat
     3421 cgtgcggatt tcgcgcaaat agcgaatcag ctcgtttcgc ttttcggtgg ctttttcagc
     3481 caccgactcc gcatggatcg ccacggggag acataaaagt agtaaaaatg ccagtctgcg
     3541 cttcataatg agaagaaccc tcgagtaagc tcttattcct atcggtggat gcaagttcaa
     3601 acctgacgca ggccaaaata aaatttacac gccggcacgg gcaagccaac gtgtaaaacc
     3661 cgatgcgcat caaacagccc gaaatatcca aggctaaagc catttcctat cgggtcaaag
     3721 acaagagcac ctgcaaggtc tgtggcttcg aattcaaccg ggaacaactt cattcgggcg
     3781 ggggccgctt gatcgccggg aaactcaccc aggagcttcg ccggctctac gaggtgagca
     3841 aaagatatgg caaggtgtat ccactggctt attccatcct gacctgtcca cagtgcctgt
     3901 tctcctcttt tccaactgat tttgctcttc ttccgccgga tgaagtagaa aaactaaaga
     3961 aggccactct ggacaggcgt acgtctgtcg agaagattgt ggggcccgtt gatttcgcag
     4021 acgatcgaaa tctggtttcc ggcgcggcca gctatctgct ggccatagac tgctatcaaa
     4081 tgcgggggat gggaatcgca cccactccca aaaaagcaat ctgcgcactt cggggtgcct
     4141 ggcttttcca ggacatggcg gaagaatttc caaagtacgg ttttgagaaa ctgagcgaat
     4201 tcctctatct taagtcagtc agctggtaca tccccactct cgaaatcatg acgaatggcc
     4261 gcgaacctca tgagcaattc atcaaccttc ttggtccgga tacggacaac aactggagtt
     4321 ttgatggtgt tatctatatc aacgggtacc tgacgcgcaa gtacctcaat cagcttgcac
     4381 ccacaaaaga aaagaagctc gaatacctgg accgaagcaa acgctacctc ggaaaactat
     4441 acggcatggg ccgcgcatcc aagagcaaac cttccgtcat catagacatg gccaaagaac
     4501 tctatgaaga attgcagaag gaaatagata caatcaacgg ggttccgccc actgctccag
     4561 ccgcatcctg attatcaccg cactgcgctt atcgtttcgt acgacggaac aaaatatgcg
     4621 ggcttccaat accagaagag cggaacaagc attcaagagt tgctggaaaa tgctttgcag
     4681 attgttgtac gcgaacatac gcgagtggat tgcgctggaa ggacagacag cggagttcac
     4741 gcaacaggcc aggtggccac attcttatct cctgttgcgc cggatcctgg gaagctcact
     4801 tattccctga attcgctgct gccttttgat gtttccgttc agaaatcgct gaacatgccg
     4861 cgcaactttc atcctcggtt ttcttgcatc gctcgcgaat atgaatacat gtatcacatt
     4921 ggcgcgcgaa gtccattctg gcgcggaagg tcctggcaca gaaagaaagg ctttgaaata
     4981 gaagaaatca atcaagagct tgcggcgatt tgcggtcata gagacttcat tgctctgaca
     5041 aaaacactct acgaaggaaa aggaacggtt cgatatctcg attcggccgt tctctacaga
     5101 aaagaagatc catggaccgg gcacggagat atgatcgttc tgcgtgttcg cgccaatgca
     5161 ttcctgcaca acatgattcg aatcctcgcc ggaacaattg tagatcgagc agcaggaaag
     5221 ctttcctcta ttacagacgc agttctgtct ggatcccgtt tcaagacagg acagacggcc
     5281 ccgtcggagg gcttgtattt cagacatgcg ttttatgtcc ccggaacagc ccccggactc
     5341 aaggaactgg ctcttccgga tgatcatccg ctcatgagga ttcatgctgg cagaatgagg
     5401 agttcccgag aaggtgacca ggaaaaccat gcgaaagagt agaatcctga tctgcatcgt
     5461 tttgctcgca gcgggcacag ccgcaagcag ccttcatgcc caggagcagc agtctgcttc
     5521 ccgcgtgaag catgagattg ggctgtggat cggagcatcc aaccccctcc ctggcactga
     5581 actggatgca gcactcgatt ccaacctggg tggaggtctg ttctatcgca tgacctggcc
     5641 ctggattctg tatacggaat tcgggacgtc ttattcgaac tatttttcgc gcgaaactca
     5701 aagcgcaact gtcatcccaa tttacgcagc gctgagttat ccaatccagc tcccgattcg
     5761 tatgcaggtt ttcttaaagg ctggcggtgg aacagcatac gtactggtgc gccctataaa
     5821 cagacatggc tgggaacctc ttggatacct tggcactgag ttttcgatcc ttgccgggcg
     5881 cagattcaga atcggtcttc gcatagacta caatctggtt tacgaaaaac atctgaaaga
     5941 tccagagcaa cctctcttac ccatcacaac tggaacaaca gatccaaggt accagaagac
     6001 gcaatatttc aagaagcgaa acggtcattt cttccacttt gggctgatga cttcttttat
     6061 tctgtgaggt ttatatgaag agatgccgct taacgttact attgatcgca ctcctgatgg
     6121 tggcatcatg caagggtaag aatgcggaac cgcttctgtc cagcctcctg gggatgaaca
     6181 gcaagactgt attgatcctg aaaggcacgt acgcatccga ctctccgctc gaattcacag
     6241 aactaaacaa caataacctc tacgtggacg cagaagatac ttctgtggat ctagtcgggg
     6301 tgcccagata cagccagctg cctatttacc tggacattgg cgagattcgc ctctcttcaa
     6361 aggattacgg aaacggactc tggagcattg atactgcgaa ggatgcgaag gctttctggg
     6421 atgtaatcgc atctgaacgc caggtctact gcagtcagcc ttactctgta agcctcgaaa
     6481 atgatacctg cttctctacg ggcggactca tcaactatca ggaatttatg aacggccgcg
     6541 gtgcgctcta cccttcaaga gacgtcgggc ccggtgtata tctccatgca ggaatttttg
     6601 ttcgcgcaat cgcaaccggc catagcaaac tggcgggcgc aattcaacaa actgcgttcg
     6661 acaacaacgt tgtaatcaac gcaaccaaca tcacttcgct tgttcagtac gatcccaatc
     6721 tgaacgatgg tctgaaacag gttctgccgc cgtactggtt tcctctccat ttcaactcta
     6781 tcatgggcgg cgtaactgct aactctatgt acatgactaa cgattacgta acaagagtcg
     6841 ttgaaatgcg tttcaacatg aaagaaaata tgatggttca tgggtttacg gatacaaatg
     6901 gctatcgctc cattgttgca ttcagtgact ggagaagagc acatgcgcag gaacgcgatc
     6961 tgggtggcaa tgtgctcatg cgcgcgcgcg tttattatcc ggactatgtg aacactctcg
     7021 tcattcaggg cggaacaaag agcaatcggc actactatgc aatcgtggat ccggttgaaa
     7081 cttatattga ggacaatctt ccagtggcgg cgacgccggt acgacagggt accaacatca
     7141 tcaagaacct gatgggtgga gtgaactaca tcatccagtg cagatttgat gcgaataatg
     7201 acgggtatcc tgaaaccatt ctggaccagc agaccttcgg tgttctaccc ggtccaggga
     7261 cagttactat tggatgccag tgcggcggcg gggtcactct ctgccattga cctctgcggt
     7321 tgactatttt cgcgatcaga atcctgcctg atttgatgca gaaacgacct gacgcaggcg
     7381 acggcgaatt tcgcgagctc gtttgcggtt ggcctcaaga atcgccgcat tgcgcgcatg
     7441 atcttcgggc gaatatctct ccgcaaacaa cttctgcaat attcgctcag taagaacata
     7501 ccaggacgcg ctggaactgg ccagaagctc tccggaaggg gaatggatgg taccgcgtgt
     7561 gtagagcctt ctctttctaa ctgcagtcag ccagcagcga acggaaacct gagtctccag
     7621 aggcatcgcg cgatggtact tcatgtgaag ttgctccgtc atcacaaaat ggcccacatg
     7681 aaagcacaat gtaccctgcg cctcatctaa aagggcggca atgacgccac cgtgcacaaa
     7741 tccaggcgcg ccttcaaaag atttttccac actgtgctga aatctcactt ctcccgtttc
     7801 gttatgaaat ggaaaatctg cgtgtagtcc tttcgcatta tcgggcccac agccgtagca
     7861 gtttgggtga tgccagtcac gcgctgtctg actgggaagc ggtttgatca tgtgtcagat
     7921 ttcagaatag acagccggcg cgcatccaat ctttatcatc gagtggatct tgatatagtg
     7981 agttttgcag tcgctcctct tggatgcaac tgctccattc tgcgggaaac ggcaaccaac
     8041 gaaaccatag tcgtggaccc gggcggagat gcggatcgaa tcattgatct gctcacagag
     8101 aagggctgca aggttaaata catcgttcat actcacgctc atttcgatca cgttcttgga
     8161 acgcatgaag tcgcttctca ttgcagaaat tcaggacacg catccgcggt atgtatgcat
     8221 ccgggggatc tccctctgta tcgaattctg ccccagcagt gcggctggtt tggtctacct
     8281 cctgtcgaag caaaagaaga aatatcacac agactggagg atgatgaagt gttgtcgttc
     8341 ggtagattca aaatgcatgt cttgcacacg cccggacaca ctcctggatc ctgctccttt
     8401 agcatcgcag agcgcggaat cctcttcagc ggggacacgt tattcgcagg aagcataggc
     8461 aggaccgatt tgcctggcgg cgactacgac caaatcataa aaagcataaa agatcgcctc
     8521 atggtactag acgacggaac cactgtgatc cccggccacg gagaattcac gcggatcttt
     8581 gatgaaaaaa gaacaaatcc ctttctctaa tctactgtgc tatttcacac tttagacttc
     8641 gcgctgttct ttggtctggt cctggccgct taccatctgg cccctacgag cttccgaaag
     8701 aatttactcc tgggtgcttc tctgtttttt tacgggtact ggaactggcg tatactaggc
     8761 ctgcttgcag taacggtaac tgcaaactac atctgcgcgc tacaattgga gaaaacgcgc
     8821 aaaagaatct ggctgatcgt agccatctgc gtcaatctcg gactacttgg cttttttaag
     8881 tattacggtt tctttgcaga gagtctgtcc ttacttctgg gccgaatgaa tccaggcctg
     8941 cccgcactga acgtactttt gccagtcggg attagcttct atacttttga attgatttcc
     9001 tacgtagttg atgtgagccg cggactaccc gcggagaaga acttccgcga tctacttcta
     9061 tttgggactt actttccaaa aatgatggcc ggcccgatcg caagggccgg tcatttgatt
     9121 cctcttttaa aggatcctcc aaaatcaacg ttaagcggta ttcagagtgg ttccatcctc
     9181 gcattcagcg ggcttttcaa gaagctagtc atcgcggaca acctggcact ggaagtaaac
     9241 agactggtgg gaaaccacgg agctcccatg agtgcactcg acatcgggaa gcacacaatt
     9301 tctgacgcac ccaccctgat actggccgca acttttttcg cggttcagat ttatgcagac
     9361 ttctccgggt attcagacat ggcccggggt atgtctcgaa tgctgggaat cgaacttgtc
     9421 caaaacttca atcatccatt tcgcgcaacc acccccgccg aattctggag acgatggcac
     9481 atcactctgg gctcctttct cagagattac atatacattc cgctcggtgg aaatcgatcg
     9541 gggatgggaa tgcagatgcg aaatctcctc attgtctgga cgattggtgg actgtggcat
     9601 ggggcttccg ccggctacgt ggcgtgggga ttctattgcg ggctgtgcct gtgcgtgtat
     9661 gtccttctgc gtccttttct ttcaggcgga ggcccgatcg ttcgtggggc aggagttctt
     9721 gctacatttc tgacttttgc actgggcctg atgatctttc gcctgggttc gcccctggaa
     9781 gtggcacgac tgctaagcat atggaaagcc ccagaaattc acccgcttgc tttcttattc
     9841 tgtcttccag tatttgtttc gcacattaat ggagacaatc agagcattct ggaaaagctt
     9901 tctcctcgcg caaaatgggc ttgcctgatt gctatgtttt acgtattaat ctttttgggc
     9961 agattttcag gagatgagtt cttctacttc cagttttgat tcagaggaaa tgctcaaacg
    10021 gcttttgggc aattcagatc gcgccccgga acgagaacgc atggtcaccg aacagatcca
    10081 gagaagaggc gtaaaggatc ctcgcgtttt gcgagctatg cgagccatcc cgcgacacgc
    10141 attcattcct gggaatctgc aaagcgaagc gtacgaagat catcctgtgt ccatcgggga
    10201 aggacaaaca atttcgcagc catacatcgt ggcgtacatg acagaacttc tagaagttaa
    10261 accaggacat tcgattcttg aaattggaac aggttgcggc taccagacgg cagtgcttgc
    10321 cattctgggc gcagaagtgt atacaatcga aatcattgcc agcctgcaac agacggcccg
    10381 gcagacgctg catgctcttg gattcgagaa cattgatttc tattgcgggg atggaagctc
    10441 aggctggcca cagaaacgaa cgtttgatcg aatcatagtc acagctgctc caccaaccat
    10501 tccacaagaa ttggtgaatc aactggcaga atccggaaag atggtgattc cggtggggga
    10561 aatgcaacaa gatcttaccg tcatgcgtcg atcaaacggg cgtctgtcgg aagaaaaagt
    10621 cctgccggta agatttgttc ctatcacaca ctgacccatt ctaaagaact cgtgcagatc
    10681 ttcaaatttt caacggaagc agtttgacgg ccccgggtcg tggtcagagt ttgtgccatg
    10741 ggccgcagcc tcgtcattct tgccttccta ctgtgctctt ctctaagcgc agaaatcgta
    10801 tatatgaagg acggccgcat tctcgaaggt tccatcgtgg cccagaacga tcagaccctg
    10861 gatattaaaa caaaaaacgg acttcagaag attcaaaagt ccaccgtgcg aagaattgca
    10921 tatcacacgg cggaagagaa agcaaaactc gaagcggaac ggaaggccag acgagacgcg
    10981 gccatgaaag aactggagcg catgagacaa ctgcgcgcgc aggaagaaga agcacgtaaa
    11041 agagcggaca tcgctcggca ggcagaactg gccaaacagc gcgccgctgc ggccaaagct
    11101 gtacgcgaaa gtgttgagcg cgaagatatg gagaaaccag acgatatcat tggtttcaaa
    11161 gacttcgcgt ggaggtctgc tctggttcca ggatggggtc atttcgcaat agggagaccg
    11221 attgttggat ccatctatgc aggcgcaacg gccggtgcac tctacaacat ctatgacaaa
    11281 aggcgcaagg cgctggccgc acgcgatgcg aaccacaacg acgttctggt caaccttctc
    11341 gtttctgtgg cacctgtgga cgctcctctt ccgttccgac ttgcctacgg gttagacgca
    11401 aaccgcaggg ctttcaccga gtacagggca cgcatcaatc gctacaatta ctccattgct
    11461 tccctcggaa ttgtctacgg agtgcagatg acccacatta tcctgaacgg tatcacgtgg
    11521 gaaagtggtt tcattgtgga cagtggggtt cgccctacct ttgacatttc gtataatgag
    11581 cataatacgg acgaacgatc cttcaaccgc gagccggaca aacaaatagt cgcgtctttt
    11641 agaatgacct tctaaagtta ttccttgaac ttcaccgtgg gcgtgagata aaaggaccca
    11701 tggccatccc tccactggac ataagcaaaa agatcgtaga cttctttgaa aaagcaacgg
    11761 tcggaacgtt aagcggcatt cgcgatatcc tggatacctc catgggtagc accgcaaatc
    11821 gtttgagcga gctcactgac aagccgctcg ttaagttcac agaaatccag ggcgcgtttc
    11881 aaaaagctgc aggccagctc agcactgcca accaggtaac tgctttcggg ctcgcaacag
    11941 ccattcaatc cgtcacagat gcattcgata gcgccggaaa tgctcttcgc atcgcagatg
    12001 aattcacgca caaacatatc tttgaaaatg tgcatatcag cagcaccgcc gggacttctt
    12061 ttgatgcgct actgaagacc aagattgttg cgtcctttcg cttgcgagat gctgattgca
    12121 gcgcccagca gatcgcagag gcagtcaaag cttcgggaaa gaccagcgcc gttctttttg
    12181 ttccggggct tttctgtgat gatggaatgt ggactcaggg accagagccc ctcctcccat
    12241 tgatcgaatc catgggatat gcatccgtat tgattcgtct gcacccaggg cttcccattt
    12301 cggagaacgg acaaaagctc gttacactcc tggatgatct gtttgcgaac atcgagatcc
    12361 cgttgcacat catcagcttc agcaacggcg gcctcattct acgcagctgt ctatactacg
    12421 cgaagctggc gggcaaacca tggttgaagc atttgcgcaa ggtcgccatc atcagccctc
    12481 cagacggcgg ttcctatatt gaaaaacttg gtttctggct gggccttgcg ctgaagtcga
    12541 ctccctctct tggtctgcaa ctgctcggct gggttggtca tatgcgcagc gatgcgatga
    12601 aggatctttc gcatgggatc attcgccagg aagacagaca tgaactcagc cagatcaaaa
    12661 ggtacggtgt ggatctatac tacaatgaac tggatgatct ggacgcgtat ctgatctaca
    12721 gcctcatatc agaaagcaat gatccggtga agacgtggct gggagacgga gtttgcgaaa
    12781 agaaaagcct tgagtatctt cgtgagtcag tgtacttgaa aaaaccaaat ccagaaaaac
    12841 gggtgcgggt catcgcgcaa agatctcatt ttcaaattct atcttctgca gaactgagga
    12901 tgcacctcaa ggaaatcttc tcgtgaaaga taaaaccatc ttcgcaaaaa tcatagatag
    12961 agaaattccc gcgtcgatcg tctacgaaga cgatcaatgt ctggccttca aagatgttca
    13021 tccccaggca ccggttcaca tactgatcgt tcccaaaaag ccaatcgacc ggcttagctc
    13081 tgcttcaggc gaagatcaat tgctccttgg ccatctactt ctgaccgcga acaaaatcgc
    13141 agagcaggcg gggctaaaag atttcagact ggtagtcaac agcggtcccg gggccgggca
    13201 gactgtgctt cacttgcatg tgcatatact gggtggacgc gagatggaat ggccgcccgg
    13261 ttagggcgac cattttgatt tatttgtaga actttagttt ctggctgcct tacttcttcc
    13321 cgctgttgta ggcagactga acgcagctcc agtaggtcat acaagcgtcg cctttggcgg
    13381 ccagagtctg cttgtagcaa accagagtct tctctttgaa ctttccgcaa gtgcgcatgc
    13441 acccgccgta catcgtttta ctctgctcgg cggtcggaac cttgttgttc tttttccaga
    13501 tgtcgacagc acattcacga tacttcgcgc aagcggcctc gcaggtcgca ggctgtgcaa
    13561 tcgcctgact gaaacccaga gcaagcagga ccacaaccag agtgctgtaa agaatcgatc
    13621 tcattttccc tccggggcaa ttccaaatgc cacctgtata gtagacggtc cccccgtcca
    13681 ttcgttcgcg caaaaccggc tttcaagccg tgcctggacc agcgacaggt tgccgtagcc
    13741 tatgccgaat gccaaaaaat caggcaaaaa aatgccattt ccgggggtac attacttgct
    13801 cttatacgca tgagcggcta accatgcggc gaaccaaata tcaggagtct ttgaatggct
    13861 aaatcaccat cagaagtcct caactgggcc aagtcggaaa aggtcgagtt tgtggacatt
    13921 cgttttaccg acatgttcgg aaccatgcaa catttcacga tgccgctaca cgcgatcgac
    13981 gaagagtctt tcacatacgg gatcggtttt gatggttcct ccatccgcgg atggaaagca
    14041 atcaacgaat cagacatgct ggctcgtctg gacgcagata cggtgtttat tgatccgttt
    14101 ctcgagcaca agacgatggc agtcgtatgc gacatttacg aaccaggagc gaatccgaaa
    14161 ccttacggcc gcgatcctcg ttacattcta aaaaaagcga tcaaatacat gcaggacagc
    14221 ggaattgctg acactgtgtg gttcgggcca gaagcagaat tctttatctt caacgatgtg
    14281 cgctttgacc agaacatgaa ctctggcttc tacttcatcg attcagacga agcatcctgg
    14341 aacatgggcc gcgatgaagg tccaaacctt ggatacaaaa tccgcaccaa agaaggatac
    14401 ttccctctgc ctccaagcga tacattccag aacattcgct ctgaaatgct gcacgaaatg
    14461 gaaaaagccg gaatcaaaac cgagaagcac caccatgaag tagcaagtcc tggacaggct
    14521 gagatcaact tcgtcgtaga cgaaggaatc aagatggcag acaaactgca actttacaaa
    14581 tacattgtga agaacgtagc gcgtcgtcat ggatactccg ctactttcat gccaaagcca
    14641 atcttcggcg acaacggttc tggcatgcac acacaccagt ccctgtggaa aggcggaaag
    14701 aatatcttcg ctggaaacaa atatgcggga atttccgatc agtgcttgaa ctacatcggc
    14761 ggtattctca gccatggacc ggctctgctc gctttcacca accctactac aaacagctac
    14821 aagcgtcttg ttccagggtt tgaagctcct gtgaatcttg tatactctgc aagaaaccgt
    14881 tctgcgtcga ttcgtattcc gatcgctgtc agtgcgagcg aaaaggcaaa gagaattgaa
    14941 ttccgtactc cggacgcgtc ttgctgccca tacattgcat ttgctgcgat gatcatggct
    15001 ggtctggacg gtatccagag caagatcgat cc
//
LOCUS       DAORFF010000013        15005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1685_length_15005_cov_4.277726, whole genome shotgun sequence.
ACCESSION   DAORFF010000013 DAORFF010000000
VERSION     DAORFF010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 15005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15005
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1685_length_15005_cov_4.277726"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKK76_01175"
     CDS             <1..473
                     /locus_tag="PKK76_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67098.1"
                     /translation="YSHIVQSMRDSVATTEPVSDVHPPYWKYLSYISQHPAQGLKFKL
                     YGDKGPGQHEVIQTNYVPPTAEEKKNKVHRTLSQHKFTIEHTRRMAIFRSHHYTAYRA
                     DFKFFNSVSIVLFDAKKADAYEEQRKAGGPKAGEAKIDPLIYKQTFKIGRPLKM"
     gene            complement(484..780)
                     /locus_tag="PKK76_01180"
     CDS             complement(484..780)
                     /locus_tag="PKK76_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67099.1"
                     /translation="MNRQWQEFKKAPGLYFRFINGKLELLGKYDRDPSQWAQIAGEIK
                     AQWEIENLREKPALDEGLQGNLFSDEDHELAGKLRTLPLLRPEKPLRPHKRRSA"
     gene            813..2336
                     /locus_tag="PKK76_01185"
     CDS             813..2336
                     /locus_tag="PKK76_01185"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNL67100.1"
                     /translation="MDGSSKHTLPVKVVTRIEPGQEDQRGIQGILNGAGLGFRYSGLI
                     DARFNFVSGFLHRLLCEVDLGYRTELVLTILRELATNCSKAHAKRIYFQSQGLDISIP
                     ADYASGMASFRADVLSHWDQFVAANSTNEFYIELSFELNADAIVIHMENNVELLPSEW
                     ERITQRRQKAAQIRDILEAFNAFDDDNEGAGLGIVFISLLLRNAGIAESNLIIDSRRG
                     VTRHTLIVPRLPAQLRSGIRDEMLREIEGLPSLPDRVNQLVVLCSDSSASIASIAQEI
                     ERDPAMAAQILRLVHSAGFMTRRRNMDVESAVKVIGLQEVSELVVATASRSVLANHFQ
                     IKEMETIWDASNRVSFFAAELAERCHVKRSDAAIAGLLLELGKIVMIGLKQTTMERID
                     EMLGSKRVRTSSILEESTLGISHPEIGARLGEKWNFPQAVIDCIRYQHKPLLCVSPNE
                     ALVRAVSLASRLHASTQGMADYYAVDPEVLQAGRVHSPEEFAKLAKLLEEQFKKQIA"
     gene            2333..3097
                     /locus_tag="PKK76_01190"
     CDS             2333..3097
                     /locus_tag="PKK76_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNL67101.1"
                     /translation="MIVAHNVRYVRQGNTILDGITWKAERNEHWIMLGPNGSGKSSFI
                     KLLTLYEWPTDGFIEIDGMRSGTEPVSVMRRKIGLFEPALHSSIAHFYPDMTGEEIVQ
                     AGARGALALYESPPEEVVHRARKLLVEERLPWERPFSVMSSGEQRRTLLMRALMPKPS
                     MLILDEPFESLDMKARYQLESTLKRNTEGMVTLTALHRIEEIPAHASHALLMKNGKVL
                     RSGPLEELLNRENLSELYEMQLEVTKHAGRYSCRPT"
     gene            3094..4809
                     /locus_tag="PKK76_01195"
     CDS             3094..4809
                     /locus_tag="PKK76_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNL67102.1"
                     /translation="MSQFSDSLCAQQMRMLRLDLPVSSEAEVARLHSKYDPWEEARRQ
                     LGQIESRPVVLAFGAGLGYAIALLLRSGKECIWFESDPRILSRALGTVDLQEFIQSGR
                     LRVVRRISNEEELEEIFRGRGNDDISFFSHRNFLTETHAEWKRKIEFFLNKKSVNMAT
                     LSRFESIWARNLVANMPLLLRGQPVSTLFGIHSNKPAIVVGAGPSLSDSIGDIEKVRQ
                     KAVLICVDTALAVLRHEGIDPDYVVTVDPQPVNRCYLEGYDGEALIVADPTASRFALR
                     HVVADRIRYFWSPFAMAQVFFEAIGVTAGEIAFGGSVSTNAYDLARKMGCDPVFVVGQ
                     DLSFPGGEVHCRGATLEERLNWKESKTFRRELHNYRQLSALPPVLLPGLTQASERTND
                     KLVIFYRWFERRFRKDLENGLRIRNCTARGARFDFLERAQLSELAGLPDAMPGPQMIR
                     ANDVSDKARKLLLVLESLEADFASTSKELELAVQIIRKEGPPKKTGETLDRVDEELRK
                     RKNALAILGNASQKAIHAVTESAHGGERALELYEALYSASLRYERWLKRSIRILSETL
                     VGIAR"
     gene            complement(4789..5892)
                     /locus_tag="PKK76_01200"
     CDS             complement(4789..5892)
                     /locus_tag="PKK76_01200"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNL67103.1"
                     /translation="MNLSYSNASSRREATLTGVSYPLRASAALLFATLSLYSCATPAP
                     VPGKACRATAGIPGPEDFDVDTSFRPPRLLISSQDRRDHDPNGELVKPGVIFQLSPAR
                     ELVGTPQPLSFADRDGLPFHPHGIAFTVLGGVPLLYVINHAMLNHHLIEVFAVKPGEL
                     KLLRRLRSPLLISPNDLVVLPNGDLYVTNDHGSSGNGFSRQVEDLLVRPWSSVVMYNA
                     ASSKWSVVADGIAFANGIAVSPSGDFLYVGSSRDRGIFVYKRTPSTGELSPRPDFIDL
                     HSGADNLMWEKPGILDIAAHPSAIAFLGHMRSSENLSPSEIFRLNTESRVAERIYSND
                     GKQISGSSTGLVWNGMLIVGQVFEPFLLTCNAD"
     gene            complement(5816..6640)
                     /locus_tag="PKK76_01205"
     CDS             complement(5816..6640)
                     /locus_tag="PKK76_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67104.1"
                     /translation="MSYALCVDTFRHPYVEFTESFRFPSVHLDRLRAEEVAEVSSLIQ
                     SLPLPWLDGFSPLPADFHPNAGPRLYWIRDVSTQDQKLAFLFRCAASYLGGCATPLGP
                     PAQGIAPSFETNRIYFHCWLVPIKDFKTDSNRITGFEADTLQLAVETRHAKPAEETVF
                     RGGSLLFDSLDFSSLNRSLSERFSFGARMAFLSVYMPFVIEHLSLCAHLLHPSPKIQN
                     TLPTMFAALLRSAPDDFASVPAYVEDFWKTYYESFVFERILSPGGNPHWRFVSTPG"
     gene            complement(6637..7131)
                     /locus_tag="PKK76_01210"
     CDS             complement(6637..7131)
                     /locus_tag="PKK76_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67105.1"
                     /translation="MRILTISLLLLFTSVHLSAQSREEALSEARAFLKKDRAAATEYA
                     SHLSGEQARALADQILFISRPGHPQIDHLNYLLSHLAQISANETGQKRLNNLLLVIVL
                     TLVLFSCFLAFVLLDQRKIIRALNASMERPPIPSSTGQLATRDTGSTPSRKSTSRKGT
                     RRKS"
     gene            complement(7133..7798)
                     /locus_tag="PKK76_01215"
     CDS             complement(7133..7798)
                     /locus_tag="PKK76_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HNL67106.1"
                     /translation="MARSSVLDFLFVAFGFALPLVSWIALSYPPTIISQGQAHRIFYI
                     HVPIAWVALYAPLLSAVSGILYLRTREQRFDLWSLASNRISLLFAAAVVISGPLWAST
                     EWGTYWNWKDSRLMSFFILLLSLVGYFYARSSTERPDRKAVVGAVMSILTSISAVLTW
                     LAIRVVTPDTHPESVMGTMAPPIRITFWISVLGYHFLFLAFLRLAIRREYLTALFEKT
                     REY"
     gene            complement(7809..8558)
                     /locus_tag="PKK76_01220"
     CDS             complement(7809..8558)
                     /locus_tag="PKK76_01220"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNL67107.1"
                     /translation="MKRFNSDTSAGRVRAGQFQKPPGFLLSLRTIYWLETKLEWRNRG
                     RIVAVAAFAVVLSVIYHYSMDQAFARSAHNLAGATLATLFFAGILLTGWNSDASDHQA
                     FRILSLSPLDASALYVGRVFARWTVLTILLIAYLPANYILLAGTLPPARTFSSQSILL
                     TGTALSLSSLGSLFNSMLTNRSRGVLLPVLLLPASVPVLVVAAGLLETFSNSPEANPL
                     LALSLLAPGILYCTLGSILFPFLNDEGPFSV"
     gene            8545..9333
                     /locus_tag="PKK76_01225"
     CDS             8545..9333
                     /locus_tag="PKK76_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67108.1"
                     /translation="MKRFIDRYAPEHLTIYIVALSAITSGYVLMTGSSLGLLQPKLLS
                     HPWDLVFFPFRLEFPGAMPALLGGGWGGLLIRLYLYWMFGSIVEAELGRVRYNVYVWM
                     GVVMVTLTGVFIPEVTAVYIYYSVFFAVAYLAPNMELYIMFILPVKIKWLAWLAVAGI
                     VLESLGSAAATGNYWLLAGPFLTFLNFFIFFGREFLETKVRAHSSGRRMKAMATVRVS
                     ALHRCTVCGMTENDDPNMEFRFCVDCSDHEYCMTHLRQHEHIRE"
     gene            complement(9338..10204)
                     /locus_tag="PKK76_01230"
     CDS             complement(9338..10204)
                     /locus_tag="PKK76_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNL67109.1"
                     /translation="MAKKVLGRGLSNLLPGESTIQIQTNPDYQELDISIVVPSPDQPR
                     KQFNPDDIRELAQTLHSVGLIEPIVVRKSGANFEIISGERRFRACKLAGFKKIPAVIK
                     QVDDIRALEMGLIENIQREDLNPMEEARAYEIWMEKTGDKPSDLAEKVGKDRTTITNL
                     TRLLKLPEDIQKLIENKMMTAGQARPLLAIADRRTSTKLADRICRENWSARRVEDEVA
                     KLQGEPGARSPVAKAAARDPNLKSVEAKLRTKLGAKVSLNHKKNGAGKLTVFYGNLDD
                     LDRILHGMGIKA"
     gene            complement(10197..10958)
                     /locus_tag="PKK76_01235"
     CDS             complement(10197..10958)
                     /locus_tag="PKK76_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNL67110.1"
                     /translation="MERIYAVANQKGGVGKTTTAVNLGGYLARMGHNVLLVDMDPQGN
                     ASSGLGVDIRELPRSVYSMLIGELSASEILQPTHIKGLSILPSNTDLAGIEPDLLQTD
                     NREYVLKRALEQLKTRYEIVLIDCPPNLGILTLNALCAASRVLIPLQTEYYALEGLTQ
                     LLDAISRVQKNFNPTLILDGVVLTMYDSRTSMSQQVVEDVRSHFHDRVYQAVIPRNVK
                     LSEAPSFGQFIGDYAPDSTGAAAYQALAKEVYDRG"
     gene            complement(10937..11647)
                     /locus_tag="PKK76_01240"
     CDS             complement(10937..11647)
                     /locus_tag="PKK76_01240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL67111.1"
                     /translation="MLEEFAKAGLPIPERLIEIVDWDRLGRFCEFLRNKNEAGGFFSK
                     GDAERILERHLYENVIFMDYVVSRCKVSRETNLADAGSGPGLPGYLFACLKQPPRITL
                     IDSSRRRLGFVEEHHRAEGSPASVRFEFSRLEEMRTTFSLIVMRALIPFPQCVELVCH
                     LVGKGGFLVLSGSRFQETDLSRFGFVSRETYVPSELSFLGARTFLLLEKTGKQDSLYP
                     RPWKIIQQEVKEWKESTP"
     gene            11712..12425
                     /locus_tag="PKK76_01245"
     CDS             11712..12425
                     /locus_tag="PKK76_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67112.1"
                     /translation="MRTLLLLSLAIPAALHAFQPGRYHPMDSYVTAGSPGGPASMVAK
                     DAKGQTLETVQFTYDGSGKLIRESFVDSKGQPAGKAEYKRNGDQLTEELRFDRTGTLL
                     SRTVFKYKDKDLIAMEVYNSQNALELRRTYTIQGGRITGGDEISDGVTDHFVMKYNDV
                     ARLLEALVFVQQDGHPFGHIAYRYDAQKRITERERVQVDKRELCRYEYDGAGRIIRFS
                     YFVMQGDWRLQKTLTFTYP"
     gene            12434..13150
                     /locus_tag="PKK76_01250"
     CDS             12434..13150
                     /locus_tag="PKK76_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNL67113.1"
                     /translation="MHEHRPYKYFDLVMAGFVTVLLCSSLIGVSKVSEFPEIHLGFLT
                     IGPFSFGSAVLFFPFSYIFGDILTEVYGYARARRVVWAGFGALFFATFMSWGVLAMPA
                     APGWPHQAAYETVFGSTWRIVLASLTAFWAGEFVNSFVLAKMKIWTEGKLLWARTIGS
                     TIAGEAVDSLIFYPVAFLGVWDTALVQKVLISNFFLKVGWEALMTPITYAAVSFLKRA
                     EHEDYYDRDTNFSPFRLDDE"
     gene            complement(13215..14648)
                     /locus_tag="PKK76_01255"
     CDS             complement(13215..14648)
                     /locus_tag="PKK76_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNL67114.1"
                     /translation="MIGELLQNLLGNAVDLVSLINIVYLVSGVLFILGLKMLGSPESA
                     RQGMFLAEIGMLLAVVGTLIDKEIVSFEWILVGAVVGTALGAVIAIWTPMTAMPQRTA
                     LSHAFGAAAVTLVGVAKFYDHPDMGAIKLSAIGFEVMFGALTFTGSMLAFLKLQGTIG
                     GSPLTFPGQNVFNISLFVAIIGLAVYVVIDPSQSAVFYTMAGLSLLFGILLVTPIGAA
                     DMPVVISLLNSYAGLAACATGFALNNKILIIAGALDGASGFILSVIMCRAMNRSITNV
                     LFGAFGGGDAAAGSAAAAGAAGPIQEMKQEDAGVMFKMAETVVVVPGYGMAVAQAQHT
                     VREFADILKKRGIKVKYAIHPVAGRMPGHMNVLLAEANVPYDELVDMEDINPEFSQTD
                     IALVIGANDVTNPAARTTPGSPIYGMPILNVDKAKTVIVIKRSMKPGFAGIDNELYTY
                     PNCYMFFGDAKDAISRLVSEMKTGGFH"
     gene            complement(14645..14947)
                     /locus_tag="PKK76_01260"
     CDS             complement(14645..14947)
                     /locus_tag="PKK76_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNL67115.1"
                     /translation="MDTATLIASLYVFVLAGFVGFLVITKVPPLLHTPLMSATNAISA
                     ISVVAAIVVAGSHHGSILPSILGCIAVASAMTNVVGGFLITDRMLKMFKKKKEEKK"
BASE COUNT         3651 a         3617 c         4138 g         3599 t
ORIGIN      
        1 actacagcca catcgtgcaa agcatgcgcg attcagtggc cactaccgaa ccggtctcag
       61 acgttcaccc gccatactgg aagtatctga gctatatttc gcagcacccg gcgcaaggat
      121 tgaagttcaa actttacggg gacaagggtc cgggacagca cgaagtgatt caaacaaact
      181 acgttccgcc tacagctgag gaaaaaaaga acaaagtgca tcgcactctc tcgcagcata
      241 agttcacgat tgagcatact cgtcgcatgg cgattttccg ttcgcatcat tacacggcat
      301 atagagcgga tttcaagttc ttcaattctg tttcaattgt gctgttcgat gcgaagaaag
      361 cagatgctta tgaagagcaa aggaaagccg gcggtccaaa ggcgggcgag gcaaaaatcg
      421 atcctttgat ttacaagcag acgttcaaga ttggtcgtcc cctgaaaatg taattaaccg
      481 aagtcatgcg ctgcgccgct tatgtggtcg cagcggcttc tcgggacgga gcaaaggcag
      541 cgttctgagt tttccagcca gttcatggtc ctcatctgag aacaagtttc cctggaggcc
      601 ttcgtctaac gcgggctttt cccgaagatt ttcgatttcc cattgtgcct tgatttcccc
      661 tgcgatttgc gcccattggg acggatcgcg gtcgtattta cccagaagct ccagtttgcc
      721 gttgataaag cgaaaatata gaccgggtgc ctttttgaac tcctgccatt gtctgttcat
      781 aaaccagagt ctatcgggcc tgtgtgacaa aaatggatgg cagttccaaa catacactgc
      841 cagttaaggt cgtgactcgc atcgagccag gccaggaaga tcaaagaggc attcagggga
      901 ttctcaatgg agctggcctc gggtttcgct attctgggct cattgacgcg cgattcaatt
      961 tcgtttctgg atttcttcac aggttgctct gcgaagtaga tctcggttat cgcacggagc
     1021 tggttttgac cattttgcgg gagcttgcga cgaattgttc gaaagcacat gcaaagcgaa
     1081 tctactttca atcgcaaggc cttgatattt ctatccctgc agattatgct tccggaatgg
     1141 catccttccg ggcggatgtg ctttctcact gggatcagtt tgtagctgct aattcaacaa
     1201 acgagtttta cattgagctg agttttgaac tcaatgcgga cgcgatcgtg atccacatgg
     1261 aaaacaatgt agagctcctt ccctcagaat gggaaaggat cacgcaaaga agacagaaag
     1321 cagcgcagat cagggacata ctggaagcat tcaatgcatt cgatgacgac aacgaaggag
     1381 caggcctggg aattgttttc atttctttac tactgcgcaa tgctggcatt gcggaatcca
     1441 atttgatcat cgactcgaga cgcggcgtga ctcgacacac tttgatcgta ccgcgattgc
     1501 ccgcgcaact acgtagtgga atacgcgatg aaatgctgcg cgaaattgaa gggcttccgt
     1561 cgttgcctga tcgggtcaat cagctggtcg ttttatgcag tgattcttcc gcgtctattg
     1621 cttccatcgc acaagaaatc gaaagagatc cagccatggc tgcgcaaata ctacggctgg
     1681 ttcattctgc cggtttcatg accaggcgac gaaacatgga cgtagaatct gcggtcaagg
     1741 tcattggcct gcaggaagtg tctgagctgg tagttgctac tgccagtcgc agcgttcttg
     1801 caaatcattt tcaaatcaaa gaaatggaaa ctatctggga tgcgagcaat cgtgtatcat
     1861 ttttcgccgc ggagcttgct gaacgttgtc atgtaaagag aagtgatgcg gccatcgcgg
     1921 gtctattgct cgagctcggc aaaatcgtca tgataggcct taagcaaact acaatggagc
     1981 gcatagacga aatgctcggc agcaaacgcg tgcgaacgag ttccattcta gaggaaagta
     2041 cgcttgggat ttcgcatccg gaaatcggcg ctcggttagg agaaaaatgg aattttccgc
     2101 aggctgtgat agattgcatt cgatatcagc ataaaccctt gctctgtgtt tctccgaatg
     2161 aggctctcgt gcgcgcggtt tcgcttgcct ctagattgca tgcaagcact caggggatgg
     2221 cagactacta tgccgttgat cctgaggtat tgcaggcggg tagagtgcat tccccggaag
     2281 agttcgcgaa gcttgcaaag ttacttgaag agcaatttaa aaaacaaata gcatgatcgt
     2341 tgcgcataac gtgcgctatg tgcgccaggg aaacacgatc ctggatggga tcacttggaa
     2401 ggcggaaaga aacgagcact ggatcatgct cggtccaaat ggatctggaa aatcttcctt
     2461 cattaagctg ctgacattgt atgaatggcc taccgatgga ttcatagaaa tcgatgggat
     2521 gcgaagtggt acggagccag tgagcgtcat gcggcggaaa atcggcctgt ttgaacctgc
     2581 actgcattca tcgatcgctc atttttatcc ggatatgaca ggcgaagaaa tcgttcaagc
     2641 aggggcgcgg ggagctctcg ccctttatga atcaccaccg gaagaagtgg ttcatcgtgc
     2701 gaggaagctt ctggtggagg agcgcctacc ctgggaaagg ccattcagtg tcatgtcgag
     2761 tggagagcag cggcgaactc tgttgatgcg cgccctgatg ccaaagccct ccatgttgat
     2821 tttggatgaa ccttttgaat cgcttgatat gaaggcgcgc tatcaacttg aatccacttt
     2881 gaagcgaaac acagagggga tggtgacgct caccgcgctt catcgcatcg aagagatacc
     2941 agcgcatgcg agtcatgctt tgttgatgaa gaacggcaag gtgctccgat ccgggcccct
     3001 cgaagagctg ttgaatcgcg agaatctttc tgagctgtat gaaatgcaat tagaggttac
     3061 aaagcatgcg ggtcgatact catgcagacc aacatgagcc agttttctga ttcattgtgc
     3121 gcgcaacaga tgcgcatgtt gcgtctcgat ctaccagttt cttctgaggc ggaggtagcg
     3181 agacttcaca gcaagtacga tccatgggaa gaggcgcggc gacaactggg gcagatagaa
     3241 tcgcgtccgg tcgttctcgc gtttggggct gggctgggct atgcgattgc gctgctcttg
     3301 cgatcgggaa aggagtgtat ctggtttgaa agcgatccgc gcattctgag tcgggcactg
     3361 ggtactgtcg atttgcagga gttcatccag tctggtcgcc ttcgggtagt tagacgaatc
     3421 tctaatgaag aagaactgga ggagatcttc cggggtcgag ggaacgatga catctcgttt
     3481 ttctcacata gaaattttct aacagaaaca catgcagaat ggaagcgaaa aatcgaattc
     3541 ttcttgaata agaagagtgt gaacatggca acgctatccc ggttcgagag catctgggca
     3601 agaaatctcg tagccaatat gcctcttctt ctaagaggac aacctgtttc tacgttattc
     3661 ggcattcatt cgaacaagcc cgcgatagta gtgggtgcgg gcccgtccct ctcggatagt
     3721 ataggcgata tcgaaaaggt tcgccagaaa gcggtcttaa tttgcgtgga cacagctctg
     3781 gccgtgcttc ggcatgaggg tattgaccct gactatgtgg tgactgtaga tcctcaaccg
     3841 gtgaatcgtt gctatttaga gggatacgac ggcgaggcgt tgatcgtggc cgatcccact
     3901 gcctcgagat ttgcattgcg tcatgtggtg gcggatcgga ttcgctactt ctggtctccc
     3961 ttcgcaatgg cacaagtatt cttcgaggcc ataggagtga ccgctggtga aatcgcattc
     4021 gggggatctg tttcgacgaa tgcgtatgat ctggccagga agatgggatg cgatccggtc
     4081 ttcgtcgtcg gtcaggatct gagctttcct ggtggagaag tgcactgcag aggagcgact
     4141 ctcgaagaga gattgaactg gaaggaaagt aagacgttca ggcgtgagct tcacaactac
     4201 agacagcttt ccgccctgcc tccagtgttg ctgcctggac tcacccaggc aagcgagaga
     4261 accaacgaca agcttgtgat tttctatcga tggtttgaaa ggcggtttcg aaaggacctc
     4321 gaaaatggtc tgcgaattcg caattgcact gctcgcggtg ctcgttttga ttttcttgag
     4381 agagctcagc tcagcgagct tgccggttta ccggacgcaa tgcccggtcc acagatgatt
     4441 cgcgcgaatg acgtttcgga taaagctcgc aaattgcttc tggttctcga atcgctagaa
     4501 gcggatttcg catccacatc aaaagagctc gagctcgcgg tgcagatcat tcggaaggaa
     4561 ggaccgccga agaaaaccgg ggagactctg gatcgcgtcg atgaagagct gcgcaagaga
     4621 aagaatgccc tggcgattct gggaaatgcg tcgcagaagg cgattcacgc tgtgactgaa
     4681 tctgcacacg gaggcgagcg agcactggag ttgtatgagg ctctttactc agcgtcgctc
     4741 aggtacgaac gttggcttaa gagatccata cggatccttt cggaaacgtt agtcggcatt
     4801 gcacgttagc aagaacggtt caaagacctg gccgacaatc agcattccgt tccacacgag
     4861 acccgtggaa gatccgctga tctgttttcc atcgttagaa tagatgcgtt cggcaacccg
     4921 gctctcggtg ttcagcctga agatttctga aggggaaagg ttctctgagg atcgcatatg
     4981 tccgaggaaa gcgatcgcag aaggatgcgc cgctatatcc aggataccag gtttttccca
     5041 catcaaattg tctgccccgc tatgaaggtc aataaaatcg gggcgtggcg aaagctcacc
     5101 cgtggaaggt gtgcgtttgt aaacgaaaat gcctctgtct cgggaagaac caacatacag
     5161 aaaatcgccg gacggagaaa cggcgattcc gtttgcgaaa gcaatcccat ccgccacaac
     5221 gctccatttt gacgacgcgg cattgtacat gaccacgctg gaccagggcc tcaccagcag
     5281 gtcctcgacc tgccgcgaaa atccattccc ggatgatccg tgatcatttg tgacgtaaag
     5341 atctccattt ggtagcacaa ccagatcgtt cgggctgatg aggagagggc ttctgagtcg
     5401 ccgaagtaac ttgagttcac ctggtttcac cgcaaagact tcgatcaagt gatggttgag
     5461 catagcgtgg ttgatcacat agagaagcgg gacaccacct aacaccgtga aggcgattcc
     5521 atgtggatga aatggcaggc cgtctcgatc tgcgaaggac aagggttggg gagttcccac
     5581 cagttcgcgc gcaggtgaaa gttgaaatat gacgcccggc tttacgagtt ctccgttcgg
     5641 atcatgatcg cgccgatcct gtgaagagat gagaagtcgc gggggtcgga agcttgtgtc
     5701 tacgtcgaaa tcttccggtc caggaattcc agccgttgcc ctgcacgctt tgccagggac
     5761 gggtgcggga gtagcgcaag agtaaagaga aagggttgca aacagtaacg ccgcgctagc
     5821 ccggagtgga tacgaaacgc cagtgagggt tgcctcccgg cgagaggatg cgttcgaata
     5881 cgaaagattc atagtatgtc ttccagaaat cttcgacata cgcgggcacg gaagcaaaat
     5941 catcgggagc ggaacgaagc aaagcggcga acatggtcgg taatgtattt tgaattttgg
     6001 gagaaggatg gaggaggtgt gcgcacagag acaggtgctc gatcacgaaa ggcatataaa
     6061 cggatagaaa tgccattcgt gcgccaaaac tgaaccgttc ggaaagggag cggttcagag
     6121 aagagaagtc cagactatca aatagaagac ttccgccgcg aaagactgtt tcttctgcgg
     6181 gcttggcgtg tctggtttcc acggccagct gcagtgtgtc cgcctcgaag cccgtaatgc
     6241 ggttcgaatc tgttttgaaa tctttgatgg gaacgagcca gcagtggaag tagattctat
     6301 ttgtctcaaa ggatggggcg attccctgcg cgggtggtcc aagtggcgta gcgcagcctc
     6361 cgagatagga agccgcacac cgaaagagaa aggccagttt ttgatcctgc gtggatacgt
     6421 cacgaatcca gtagagacga ggaccggcat tcgggtgaaa gtcagctggc aaaggtgaaa
     6481 agccgtcgag ccagggaaga ggaagagact gaatgagaga cgaaacctct gctacttcct
     6541 ctgctctcag tcgatccagg tgaacggacg gaaaacgaaa tgattctgta aactcaacgt
     6601 atggatgtct gaacgtatcg acgcacaggg cgtagctcac gacttccttc tggttccttt
     6661 gcgtgaagta gatttcctgg aaggcgtcga gccagtgtct cgcgtggcca gttgcccggt
     6721 cgaggatggg atcggaggcc tttccatgga cgcgttgagt gcgcggatga tcttgcgctg
     6781 gtctagcagt acgaaggcaa gaaagcacga aaacagcacg agtgtaagca cgattactag
     6841 aagcaggttg ttcagtctct tctgtcccgt ttcatttgcg gaaatctgcg cgaggtgcga
     6901 aagaaggtag ttgagatgat cgatctgcgg atgaccggga cgagaaatga ataggatctg
     6961 gtctgcgagt gctcgcgctt gttccccgct gagatgggag gcatactccg tggcagccgc
     7021 gcgatccttc ttgaggaagg cacgcgcctc ggatagcgct tcttcgcgcg attgcgcgga
     7081 gagatgaacg gaagtgaata gtagtaaaag actgatagtt aagattcgca tattaatatt
     7141 cccgggtctt ttcaaaaagt gcggtcaagt attcgcgtcg aatggcaagg cggaggaatg
     7201 ccagaaacag gaaatggtaa ccaaggacgg atatccagaa tgtaatgcga atgggagggg
     7261 ccatggtgcc catcacggat tcgggatgcg tgtccggtgt gaccactcga atcgcaagcc
     7321 atgtaagcac agcggagatc gacgtgagta tggacatgac agctcccacg acagcttttc
     7381 tgtcgggtct ttcagtggag cttcgcgcgt agaagtagcc aacgagtgaa aggagtagaa
     7441 tgaaaaagga catgagccgc gagtctttcc agttccagta ggtgccccat tctgtcgagg
     7501 cccagagagg tcctgagatc actacggccg cggcaaatag gagagaaata cgattggagg
     7561 cgagagacca gaggtcgaac cgttgctcgc gtgtgcgaag atacaatatg ccgctcactg
     7621 ctgaaaggag aggagcatac agagcaaccc atgcgatcgg aacgtgtatg taaaagattc
     7681 tatgtgcctg accctgtgag atgatggttg ggggatagga aagagctatc cagctaacga
     7741 gtggtaaggc gaagccaaaa gctacaaata ggaaatcgag aacactggat ctggccatgc
     7801 gagcaaggtc agactgagaa aggcccttca tcgttaagga aaggaaataa gatcgatccc
     7861 agagtgcaat acaatatgcc cggtgcgagc aaggatagag cgagaagagg attcgcttct
     7921 ggagagttgc tgaaggtttc gagtagcccg gctgccacga ccaggacagg aacagaagcg
     7981 ggaaggagta atacaggcag aagcacccct cttgatctgt tggtgagcat cgagttaaaa
     8041 agtgaaccca gagaggaaag actcaacgcg gtgcccgtga gcagaatgga ttgcgaagaa
     8101 aatgtacgag cgggtggtag ggtgcctgcg agtaagatgt agttcgccgg aagatacgcg
     8161 atcaatagaa tcgtgagcac tgtccatcgt gcgaagacgc gacccacgta gagcgcactc
     8221 gcgtcaagcg gcgagagaga caggattcgg aatgcttgat gatctgaagc atcagaattc
     8281 cagcccgtca gaagaatgcc tgcaaagaac agagtggcca gagtggctcc tgccaggttg
     8341 tgcgcggacc ttgcgaaggc ctggtccatc gagtagtgat agatgacgga gaggaccaca
     8401 gcgaaggcgg ctactgcaac gatgcgtccg cggttgcgcc attctagttt cgtttcgagc
     8461 cagtagatcg tgcgaaggga caagaggaat ccgggaggct tttggaattg accggcgcgc
     8521 actcgtcccg cgctagtgtc gctgttgaaa cgcttcattg accgatacgc acccgagcac
     8581 ctgaccatct acatcgtagc tctgtccgcc atcaccagcg gatatgtttt gatgaccgga
     8641 agcagcctgg ggttactgca acccaaattg ctctctcatc cgtgggacct agtctttttt
     8701 ccgtttcggc ttgagtttcc aggcgcgatg ccggccttgc taggcggtgg atggggaggc
     8761 ctgttgattc ggttgtatct gtactggatg tttggatcca ttgtggaagc ggaactcggc
     8821 cgggtcagat acaacgtcta cgtgtggatg ggcgtcgtca tggtgacgct gaccggggta
     8881 ttcattccgg aagtaacagc tgtctacatc tattattcgg tgttctttgc ggtggcatat
     8941 ctcgcgccca acatggagct ctatatcatg ttcatattgc ctgtgaagat caaatggctt
     9001 gcgtggctgg cagtggcggg cattgtcctg gaatcgcttg gaagtgccgc ggccaccggg
     9061 aactactggc ttcttgctgg cccatttctc acattcttaa atttctttat attctttggc
     9121 agagagtttc ttgagaccaa ggtccgcgca cattcttctg gtcgtcgcat gaaggcaatg
     9181 gcgaccgtgc gagtgagtgc acttcatcgg tgcactgtgt gcggcatgac agagaacgat
     9241 gatccgaaca tggagttcag gttctgcgtg gattgttcag accatgagta ctgcatgact
     9301 catctgcgac agcatgagca catacgagaa taagatccta tgctttgatt cccatcccgt
     9361 gcagaatacg atccaggtcg tccaggtttc catagaatac agttaacttg cctgcaccgt
     9421 tctttttgtg gttgaggctc accttcgctc ccagtttggt tctgagtttt gcctctacgg
     9481 atttcaaatt tggatcgcgt gccgcggctt ttgctacagg ggatcgtgcg ccaggttcac
     9541 cctggagttt tgcgacttca tcttccactc gtctggcaga ccagttctct ctgcaaatgc
     9601 gatctgcaag ttttgtcgag gtgcgtctgt ctgcgatggc aagcaaggga cgagcctggc
     9661 cggcagtcat catcttgttc tctatgagtt tctggatgtc ttcggggagt ttcagaagcc
     9721 gcgtgagatt ggtgatcgtc gttcgatctt tcccaacttt ctccgcaaga tcggatggct
     9781 tgtctccggt cttctccatc cagatttcgt atgcgcgggc ttcttccata ggattgagat
     9841 cttctctctg gatgttctcg atgaggccca tctctagagc gcggatgtca tccacttgtt
     9901 tgatgactgc ggggatcttt ttaaatcctg cgagtttaca tgcgcggaac cgtcgctcac
     9961 ccgagatgat ctcgaagttg gcgcctgatt ttctgactac tatgggttca atgagaccga
    10021 cgctatgcag ggtctgagcg agctcgcgaa tatcgtctgg attgaattgc ttacgcggtt
    10081 gatccggaga tggaacaaca atggagatat ccagttcctg ataatcaggg ttggtctgga
    10141 tttgaatggt ggattcgccg ggcagaagat tggatagtcc gcgccccagt acttttttag
    10201 ccacgatcgt atacctcctt tgcaagtgcc tgataggctg ccgcgcccgt tgagtctggc
    10261 gcatagtctc caatgaattg tccgaatgaa ggagcttccg agagctttac gtttctcgga
    10321 atgactgcct ggtagacgcg atcatggaaa tgagagcgaa cgtcttcgac cacctgttgt
    10381 gacatggacg ttcgagaatc atacattgta agaaccacac cgtccagaat gagggtgggg
    10441 ttgaagtttt tttgcactct agaaatagca tcgagcaatt gagtgagtcc ttcaagagcg
    10501 tagtattccg tctgcaatgg aatcagtact ctacttgcgg cgcagagtgc gttgagggtg
    10561 aggatgccca ggttaggcgg acagtctatg agcacgattt catatctggt cttcagttgt
    10621 tcgagagctc gcttgagcac atactctctg ttgtccgttt gcagaagatc cggttcaatt
    10681 cctgcaaggt ccgtgttgga cggcaagatg gagaggcctt tgatgtgagt tggttgcagt
    10741 atttctgaag cggataattc cccgatcagc atgctgtaga cggagcgcgg aagttctctg
    10801 atgtctactc cgagcccgga ggatgcattc ccctgagggt ccatgtccac gaggaggaca
    10861 ttatgtccca ttcgcgccag gtatccaccg aggttcacag cggtcgtagt tttgccaact
    10921 ccgcccttct gatttgctac ggcgtagatt ctttccattc cttcacttcc tgttggatga
    10981 ttttccaggg cctggggtac aggctatcct gtttccctgt tttttccagg agcaggaacg
    11041 ttctggctcc gagaaaagac agctcagatg ggacataagt ttcacgtgaa acaaaaccga
    11101 acctggacag gtccgtttcc tgaaatcttg atcccgagag cactagaaat cctccctttc
    11161 ccacaaggtg gcacaccagc tcaacgcact gcggaaacgg aatgagtgcg cgcataacaa
    11221 tcaggctgaa cgtggttcgc atttcttcca ggcggctgaa ctcgaaacga acggaagccg
    11281 ggctcccctc tgccctgtga tgctcctcaa caaagcccaa tcttcgcctg gaggaatcaa
    11341 tgagcgtaat ccgcggtggc tgttttaaac acgcaaagag atatccaggt aggccagggc
    11401 ccgaaccagc atctgccaga ttagtttcac gtgaaacctt gcatcgtgat acgacataat
    11461 ccatgaatat gacgttttcg tacagatggc gctccagtat tctctccgca tctcccttcg
    11521 aaaagaatcc ccctgcttca ttcttgtttc tgagaaattc acagaatcgt ccaagtcgat
    11581 cccagtccac gatctcaatg agccgttccg ggatcgggag gccggctttt gcaaattctt
    11641 cgagcatgca agcatccttc ttacctccgg ttttcggtca actccagcgc ccttcctccg
    11701 atacataagt ggtgagaaca cttctgctcc tgagcctggc gatcccggct gccctccatg
    11761 cctttcagcc agggcgctat catcctatgg attcctatgt cactgcggga tctccaggcg
    11821 gcccggcgtc gatggtcgcg aaagacgcga aaggccagac tctcgaaacg gttcaattca
    11881 cttatgatgg ttccggaaag ctcatccgcg aatcatttgt agattcgaaa ggtcaacctg
    11941 ccggtaaggc ggagtataaa cgcaatggcg accagctgac agaggaactt cgatttgatc
    12001 gcacgggcac tctgctgtcg cgcacagtct tcaaatacaa agacaaagac ctcatcgcga
    12061 tggaagttta caactcccaa aacgctctgg aactcaggcg tacctacaca atccagggtg
    12121 ggagaattac aggcggtgat gaaatttcag acggagtcac ggatcacttc gtgatgaagt
    12181 acaacgatgt agcccgactc ctggaagctc tcgtatttgt tcaacaagac ggtcatccgt
    12241 tcggtcacat cgcgtatcgt tacgatgcgc aaaagcggat cacggaacgc gagagagtgc
    12301 aggtcgacaa aagggaattg tgtcgatatg aatatgacgg cgcgggacga atcattcgct
    12361 tctcatactt cgtgatgcaa ggagattggc gccttcagaa gactttgaca tttacttatc
    12421 cctgaggcac cacatgcacg aacaccggcc ctacaaatat ttcgatctag tcatggccgg
    12481 ttttgttacc gtgcttcttt gttccagttt gattggcgta tccaaggttt ccgaatttcc
    12541 ggagatccat ctgggcttcc tgacgattgg tcctttcagc tttggctcgg ccgttctttt
    12601 ttttccattt agctacatct ttggagacat actcacagaa gtatatggct atgcgcgggc
    12661 gcgcagagta gtatgggccg ggtttggagc tctgttcttt gcaacattca tgagctgggg
    12721 agtgctggca atgcctgcgg cgccagggtg gcctcatcag gcagcatacg aaacagtctt
    12781 cgggtccaca tggcgaattg tgctcgcaag tcttaccgca ttctgggccg gcgagtttgt
    12841 gaactcgttc gttctcgcaa agatgaagat ctggacggaa ggcaaattgc tctgggctcg
    12901 taccatcggt tctacgattg ccggagaagc agtggactct ctgattttct acccggtggc
    12961 cttcctcgga gtatgggaca cagctctagt acagaaggtt ttgatttcca atttcttctt
    13021 aaaggttgga tgggaagccc tcatgactcc tatcacgtat gctgctgttt cttttttgaa
    13081 gcgagcggag cacgaggact attacgatcg ggacaccaat tttagtccgt ttcgtctgga
    13141 cgacgagtag cgcgggtttc cccgcgccct ctggttcgcg taactggcgt taggcgaacg
    13201 agttgctctg tggcttagtg aaagcctcct gttttcattt ctgacacgag cctgctgatg
    13261 gcatccttcg catctccaaa gaacatgtag cagtttggat atgtgtagag ttcgttgtca
    13321 atgcccgcaa agcctggctt catgcttcgt ttgatgacga ttacagtttt ggctttgtcc
    13381 acgttgagga tgggcatccc ataaatcgga cttccgggag tcgtgcgcgc ggctggattc
    13441 gtcacgtcat tggccccgat cacgagtgca atgtctgtct gagaaaactc aggattgatg
    13501 tcttccatgt ctacgagttc gtcgtagggt acgttggctt ccgcaagtag aacgttcatg
    13561 tgacctggca tgcgcccggc cacaggatga atggcatact tgactttgat cccgcgtttc
    13621 ttcaaaatat cagcaaactc tctcacagta tgttgggcct gggccactgc cataccgtac
    13681 ccgggaacga ccaccacagt ctcagccatc ttgaacatga cgcctgcatc ttcctgcttc
    13741 atctcctgga ttgggccggc tgcacctgca gctgcggctg agcccgctgc tgcgtcaccg
    13801 ccgccgaatg ctccaaacag aacgttcgtg atcgaccggt tcattgcccg gcacatgatc
    13861 acagacagga taaaaccgga agcgccgtcc agtgcacccg caatgattag aatcttgttg
    13921 ttgagtgcaa acccagtagc gcaagccgca agaccagcat aagagttcag gagtgaaatc
    13981 accactggca tgtctgcggc accaatcggc gtcacgagaa ggataccaaa cagcaggctc
    14041 agtccagcca ttgtatagaa gacagcagat tgactgggat cgatcactac ataaaccgcc
    14101 aacccgatga tcgcgacaaa cagcgatata ttgaagacat tctggccagg gaaggtgaga
    14161 ggagagccac cgatcgttcc ttgcagtttt aagaacgcaa gcatactacc agtgaaagtg
    14221 agcgcaccaa acatgacttc gaaaccaatc gcagagagtt tgattgctcc catatccgga
    14281 tgatcgtaaa atttcgccac acctactaga gttacggcag ctgcaccgaa tgcgtgcgaa
    14341 agtgcagttc gctgcggcat cgcagtcata ggagtccaga tggcaatgac ggcacccagc
    14401 gccgttccta caactgcgcc caccaggatc cattcgaaag atacaatttc cttgtcgatc
    14461 agagtgccca cgaccgcaag cagcatgccg atctccgcca gaaacatacc ctgtcgggcg
    14521 gactcaggtg atccgagcat ttttaacccg agtatgaaca ggactccgga tactaaataa
    14581 actatattaa ttaagctgac cagatctacg gcattgccga gaagattttg aaggagctcg
    14641 ccaatcattt cttttcctct ttcttcttct taaacatttt gagcatacgg tcagtgatca
    14701 gaaagccgcc cacaacgttt gtcatcgcag aggccacagc gatgcatccc aggatgctcg
    14761 gcaaaatgga accgtgatga gatcctgcga cgacgatggc tgccacaaca gaaattgcgg
    14821 aaatcgcatt tgttgccgac atgagtggag tatgcagcag cggcggaact ttcgtaatca
    14881 cgagaaatcc aacgaatccc gcgagcacga aaacatataa acttgcaatg agagttgctg
    14941 tatccaattg tgaatcctcc tgtttacgcg cctatcgctg cgcgagtagc ttcatgaatg
    15001 atttg
//
LOCUS       DAORFF010000014        14961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1698_length_14961_cov_3.776063, whole genome shotgun sequence.
ACCESSION   DAORFF010000014 DAORFF010000000
VERSION     DAORFF010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14961
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1698_length_14961_cov_3.776063"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="PKK76_01265"
     CDS             complement(<1..1057)
                     /locus_tag="PKK76_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNL67116.1"
                     /translation="MNELFSESTELDRKLGLKETSAIVVGRIIGSGIFRTPSSIFALV
                     GCSSLFYGVWILGGLVSLMGAFCVMEMIAMMPRSGGPYAFLKAAYPPVWTFLRGWAMF
                     FVAETGAIAAVALVFAEYSEFLSKELGGPLFGIAGRTAIALMLVWSWTAANCFGVQLS
                     GRLQVVFSSAKVLALLSVIVAGIALRPDPDHIANNFWPQNGGIGTVLAFFAAMRYGFF
                     AYSGWEGATYVAEEVKNPRRNLPLSLLLGISGVMLLYLAANTGYLLQLTGSEMHAARQ
                     YIAADAMRKALGAIGGVLIAAAVMINTFGNVGTQILVKARTWFAMARDGLFFPRLGEL
                     SAIYHTPNRALIAQAFWA"
     gene            complement(1054..3618)
                     /locus_tag="PKK76_01270"
     CDS             complement(1054..3618)
                     /locus_tag="PKK76_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNL67117.1"
                     /translation="MNSNNGSLQSTNNSTKVMTGFRKAAPGAVFTIAVLSFSTLGAQE
                     SFNPASVMPAECRNGSYHCGFLPPNPDEYRSLPMVDSAFYKERGLPSSVDMSADMPPV
                     GFQGDQGSCLGWSLAYAIRSYQEKKAFGWSYDSPIYGGQGTRVMSPSFIYNQINGGRD
                     QGSDPIAALRLLVTRGVSTWKRMPYSLGDYRSQPGPEALAEAMQFRSRGFRAIDSAAV
                     DSIRTELSKGRPVMLGILAYENFYKLDKSNPVYDDFRGKFFGGHAVTIVGYDDSKDTP
                     GGRGAFKVMNSWGRAWGENGFAYISYRVFPKIVTAGFVIEQRDKASASPPSLEHLLLA
                     PREVAATRGVFEDKVELTWTSADGAVAYEIQRSSMRFERIGFSVAPRFTDNNVEKGIP
                     YKYRIIAIYRTGRSDPELSPTAEGFARNPGIFERYVLPPVLDIVANSLGTAFRSLVTP
                     DALPSQMTQTPAAASTNGITLSWNQTSGAEWYDIARYEPALKSFQLLAVSRETTFRDP
                     AGRMDAVYSIRARSRLTSGAWSAPIMAGNGVTRLDASRGLYRDRITVKWDPVPGANGY
                     IIHFYDATKQKYTGSTKTTSNQYTDESERAKSGSWTGYTVAALFEDQTGKFTGPVFGK
                     SNPALNRMILPPPSGLAVEVTRDGAQIQWNQVAGAERYFVFRKRERDSDFAMYAEVRA
                     NQFRDKELKAGELYYYTVRASNEEGESLDSQSLAAALPGNLLALVPQQPAPPNAGQRA
                     SRVSEDSARTESSKISQNIGAGDPFARNWKGSYWDGKELSPVSVKVNMKGEIAEAVVQ
                     FAGRPAQIIKGIHPAGAQIFEIPGIKATLSRDALMLRFTDNNIAPYPIQVAMDPSP"
     gene            complement(3581..4498)
                     /locus_tag="PKK76_01275"
     CDS             complement(3581..4498)
                     /locus_tag="PKK76_01275"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNL67118.1"
                     /translation="MDLSTIAYHKSEFKKLSDCTVNVATHALQYGTAVFGGVRGYYSK
                     GMDNLLIFRIDRHFKRLHQSAKMMRMTPPLSEKEMANILIQVARFNNYRQNVYFRPFL
                     YKAAEQLSPRFHDVKDEFSLYSLRLDDYLETNKGLSACVSSWRRIDDNIIPTRGKVSG
                     GYANSGLAKSEAVENGYDEAIFLDLRGFVCEGSAENIFMVRDGVLVTPPVAASVLEGV
                     TRRSVLELAKSEGIPVEERDIARAELYVADEVFFTGTGAQIAWVSSIDHRVIGTEVGP
                     VTNKLRSRFLKCVLGEDSEFKQWVTPVYE"
     gene            complement(4564..6279)
                     /locus_tag="PKK76_01280"
     CDS             complement(4564..6279)
                     /locus_tag="PKK76_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNL67119.1"
                     /translation="MLISRLSIRARIWMLALGFFATFLFAGVFVAVSEMALSSRVNDA
                     VARMELVRQSQVHFKLQVQEWKNILIRGSDPEAYKKHLIAFRTESTAFQALLQKLIES
                     ETNSDTKSKAIEIQKRHSDLLKQYEVTLEELSAGSPDFTRAADRRVKGMDRPTDQQLS
                     ALVELISNQSKGNQERDLRLFWISLGSAGLIVTVLTFLFSWMAVRSVSNTLGAVTGTL
                     QQISEGKLNLEIDDSGSDERSTILSAVKRSSRKIAAMLLSVRDQVHQTRGAAFEISGT
                     AKKLMKASQNLAASSEESAAAVEELSAISDHVSDSMTVVAQNSKEVDHSIHQLATESE
                     EMRMSMQTLGARNLESSNRARSGLQQIQIVSESMEQVKDGSQKIGAIMSLMHELSDRI
                     NLLALNASIEAARAGEAGRGFAVVAEEVSRMADRTMARIKEVETNTSSIQSAILESHT
                     QVLAAADSFGVMMRDLLQMGDSVSGFATRVDEQVSRVSAIAASMQQLSNAASTVEGAA
                     KESKNAARELAESTRTNASEADKILHESEALQEVSGNMRTRADQLGQIMDEFSLDESN
                     QASNR"
     gene            complement(6428..7681)
                     /locus_tag="PKK76_01285"
     CDS             complement(6428..7681)
                     /locus_tag="PKK76_01285"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNL67120.1"
                     /translation="MAESADELNPYLIACKQISRAAEILKLPRSVTEFILRPRRVLIM
                     NFPVRMDDGSVRVFEGFRAQHTDALGPAKGGIRFHPNVTLDEVKALSMWMTFKCGVVD
                     LPFGGGKGGVVCDPRSLSHGELERLSRAYAEAASVILGPEIDIPAPDVYTTPEIMGWM
                     MDTLSRLRGYNVPSAITGKPIVLGGSKGRDRATAQGCLYIVREYLRDRKIEPANARIV
                     IQGFGNAGRTAATLLSELECKIVAISDSKTGLQDPTGLNIQKACEAKDAGGLHLYGSG
                     FMIDSKLALQCEGDVLIPAALENSITSENAHHIQCKAVIEAANGPTTPEADKILSSRG
                     VQVIPDILANAGGVTVSYFEWVQNVTHSAWEETEVASRLEKRMIDAYHAVQKSARAHG
                     ADLRTAAYLISVERLRDAIVARGWA"
     gene            7760..8575
                     /locus_tag="PKK76_01290"
     CDS             7760..8575
                     /locus_tag="PKK76_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNL67121.1"
                     /translation="MSIHLLKRRIPRGGELLPVIGLGTWQSFDKEPSEELSKVLATFY
                     EGGGKLIDSSPMYGKSEETVGSLIATRRKDFFVATKVWTRGEKQGFAQMEESFRKLQT
                     RKVDLMQIHNLVDWRIHLHTLRMMKEQGRIRYIGVTHYAPSSFGELTLAIRDRAVDFV
                     QLPYSAFTRTAEDGILAEAADRGVAVIVNRPFEEGALLRRLRAKALPTVAAELGCFSW
                     AELLLKFILARPEVTCVVPATSNPVHMREILRAGTGRLPDERERESIAMAARG"
     gene            complement(8579..10009)
                     /gene="pyk"
                     /locus_tag="PKK76_01295"
     CDS             complement(8579..10009)
                     /gene="pyk"
                     /locus_tag="PKK76_01295"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNL67122.1"
                     /translation="MNRAKLTKIIATLGPASSSEDAIRSLIRAGADCFRLNFSHADGP
                     AMQPLIDRVRAVSQSEGVPIAILADIQGPKLRIGKLADEGVLLVEGKTFTITTREITG
                     TDQIVHSPYEFLTRDVKPGSRLLLADGTIELLVENVTSTDVNARIVRGGQLFSNKGLN
                     LPGTHLSVETLTEKDRKDLAYIAATDIDVVAISFVRRAEDIHKARECLGSSRIPIMAK
                     LERPEALQNIEDILRAADGIMIARGDLGVEVEFERVPFIQKEILHRAAQRGKWAIVAT
                     EMLRSMVRANRPSRAEVTDVANAVLDGTDAVMLSEETAVGQHPSLVVEAMLRITKEAG
                     EHQNIEKPSFEADMVSFSAGAAGAAVSAAERLRARAIIVLAGSNLTALLLSKWRSKIP
                     ILALASGLPTLRRLNVLRGVTPVSIRDNSEMDEQIAEADRYLVEHGFATPGETVVFVA
                     ALPLGEGKETNSIRFHRVRATPSPWQ"
     gene            complement(10066..11007)
                     /locus_tag="PKK76_01300"
     CDS             complement(10066..11007)
                     /locus_tag="PKK76_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNL67123.1"
                     /translation="MRAIAYKKYGKAEVLEEMDLPDPVMGEDDLLVRIHAVSVNPIDW
                     KIRSGALRLLSGGNFPRIPGSDFAGEVLSTGKNTRRFQKGDRVFGMLPPMKGGAFATL
                     AAVPERLAAQAPRNLTYEECAAVPLAGLTALQALRDPGEIQDSQKVLIYGAAGGVGTF
                     AIQIARIFQADVTAVASGSSEEIVRSAGANRFIDYTRENFARIDSKYDIVFDCVGKTY
                     YADCVDILKPDGKYITVELGPGVFWDSLRGGFFGGPTCSFIITKESSDDLDMLRHYIQ
                     GKTIKPVISTIFPLSRAREAHALSEAGHVKGKIVLRM"
     gene            11106..12008
                     /locus_tag="PKK76_01305"
     CDS             11106..12008
                     /locus_tag="PKK76_01305"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNL67124.1"
                     /translation="MLVRFFFLGLLLNSSCALLVRKGSVAEIQQIAVQGQVRRYILHV
                     PASYDAQKKTALVVVLHGGGGNGRNAEKMSGFSAKADKEGFIVVYPYGSGRLDERLLT
                     WNAGNCCGYALDKNVDDVAFIRELVVLLKRQYSIDDKQIFATGMSNGGMMTHRLACEM
                     SDVFSGVAPVSGAMNVPVCNPARAVSMVIFHGKKDEHVLFEGGAPKKSVDSHPRVDKS
                     VAAAVEFWKNRNGCAQSPTESHGKIQIEDFSCSQAGLAVYAIEDEGHTWPGGEKGYFA
                     ADEPTRELSATDVMWDFWQKNSRP"
     gene            complement(12213..12791)
                     /locus_tag="PKK76_01310"
     CDS             complement(12213..12791)
                     /locus_tag="PKK76_01310"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNL67125.1"
                     /translation="MTRNRLEAFSDGVIAILITIMVLELKVPHGSDLTQLLPLVPVFL
                     SYVLSFLYLGIYWNNHHHMLHLANRINGKVLWANMHLLFWLSLIPFVTAWSGENHFET
                     NTVVAYAFVLLMCGAAYYILEQMLIAAEGKDSALAKAVGNDRKGIASLVLYILSIAIA
                     FYNSQAAMAVIVIVAIMWIVPDTRIEKHFNKR"
     gene            complement(12833..13909)
                     /gene="prfA"
                     /locus_tag="PKK76_01315"
     CDS             complement(12833..13909)
                     /gene="prfA"
                     /locus_tag="PKK76_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNL67126.1"
                     /translation="MSAVKDRLEKELLRVREIEDLLSQPDVTSNPARFKELAREHSIL
                     SARVEPIERFLKILKDQDEASRILSDKTADQDLRDMASEEKVRLDQELAAGRDEMEAL
                     LLPPDPNSGKSVIVEIRAGTGGEEAALFADDLFRMYMRYVERSAMKLEVLSTVESDLG
                     GYKEIAFSIDGKEAYDLFHQEAGTHRVQRIPTTESGGRIHTSAVTVAVLAEAEEEEIS
                     IDDKDLQIDTYRASGAGGQHVNKTDSAIRITHIPSGLVITCQDERSQLKNKSKAMKVL
                     RARLLERQKSSRHAEEAALKKDQVGSGDRSEKIRTYNFPQNRLTDHRIGFTSHNLSAM
                     LDGDIEELLRALVRAEREQKLAAV"
     gene            complement(13919..13991)
                     /locus_tag="PKK76_01320"
     tRNA            complement(13919..13991)
                     /locus_tag="PKK76_01320"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13955..13957),aa:Arg,seq:cct)
     gene            14073..14441
                     /locus_tag="PKK76_01325"
     CDS             14073..14441
                     /locus_tag="PKK76_01325"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 family protein"
                     /protein_id="HNL67127.1"
                     /translation="MSLLFHFLHIAVMAANLFAWTIPRLRRFHLIVVGCTLFSWCVLG
                     LRYGFGYCFLTDWHWSVLESQGVRNLPGSYVKYLFDLITGWDSSPFWIDVATGVSFSI
                     AISSSLYVNRDLFRFLRPTS"
     gene            complement(14436..>14961)
                     /locus_tag="PKK76_01330"
     CDS             complement(14436..>14961)
                     /locus_tag="PKK76_01330"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNL67128.1"
                     /translation="RSIYQYRYNQKHKKRRVHFLRESFLVTLVAVCWWGSTGLYVFTN
                     FLENGSFHLHTIARIAGGCIMFASIVYFWRVHSFLGKHWSPVLEIEHEHTIIETGPFR
                     TVRHPMYSAIIVSILSQGLLTANLFVLIPSLVSFLFLCWIRIPAEEKMLEEEFGEEYA
                     KYKSRTWIFFPWVL"
BASE COUNT         3383 a         3847 c         3801 g         3930 t
ORIGIN      
        1 tggcccagaa cgcctgagca atcaatgcgc gattgggtgt gtggtagatg gcactcagtt
       61 ccccgaggcg cggaaaaaaa agaccgtcac gcgccattgc aaaccatgtg cgagctttca
      121 caagtatttg tgtgcccaca ttgccaaagg tattgatcat cacagcggcc gcgatcagca
      181 cccctcctat tgcacccagg gcctttctca ttgcatccgc cgcgatgtac tggcgagcag
      241 catgcatttc acttccagtc aattgtagca aataaccagt gttagctgcc agatacaaga
      301 gcatcactcc ggaaattccg aggagaaggg agagaggaag attgcgtcgc ggattcttca
      361 cttcttcggc aacataggtg gcgccctccc aaccgctgta cgcgaagaag ccatatcgca
      421 tggctgcaaa aaacgcgagc acagttccaa ttcccccatt ttgcggccag aagttgttcg
      481 cgatgtgatc cgggtcaggg cgaagagcga tgcctgcgac tattacagag aggagggcaa
      541 ggacttttgc ggaactgaac actacctgca atctaccact gagctgcacg ccaaaacaat
      601 ttgcggccgt ccatgaccag acaagcatga gtgcaatggc agttctgccc gcaatgccga
      661 ataacggtcc tcccagttct ttggaaagaa actctgagta ttcagcgaac acaagtgcta
      721 ccgcggcgat agctccagtt tccgccacga agaacatggc ccagccgcga agaaatgtcc
      781 agaccggagg ataagccgcc ttcaagaaag catagggccc tccggagcgc ggcatcattg
      841 caatcatttc catgacgcag aatgcaccca tcagagacac aaggcctcct agaatccaca
      901 cgccgtaaaa gagagaagag cagccaacga gtgcgaagat ggaggagggc gtgcgaaaga
      961 tccctgatcc tatgattcta ccaaccacaa tggcggatgt ttctttgagt ccgagtttgc
     1021 gatccagttc tgtggattcg ctgaaaagtt cgttcacgga gatggatcca tcgccacctg
     1081 gatcgggtag ggtgcaatgt tgttatcagt aaatcttagc atgagcgcat ctctgctgag
     1141 cgttgctttg attcccggga tttcgaaaat ctgcgcgccc gctggatgaa ttcctttaat
     1201 gatttgtgcc ggtcttcccg caaactgaac tacggcctct gcgatttcac ctttcatatt
     1261 tacttttaca gacacaggcg agagttcctt tccatcccag taagatccct tccaatttcg
     1321 cgcaaacgga tcaccggccc ctatgttctg gctgattttt gagctttccg ttcttgcaga
     1381 atcttctgat actcgagatg ctctctgtcc cgcattcgga ggagctggct gttgcggaac
     1441 gagcgccaga agattgcccg gcaaagcggc agcgagcgac tgagaatcga gcgattcgcc
     1501 ttcctcattg ctggcacgga cagtatagta gtagagttct ccggccttta gttccttgtc
     1561 tctgaattga ttggctcgca cttctgcgta cattgcaaaa tcagagtcac gctctcgctt
     1621 gcggaaaaca aaataacgtt ctgcgccggc cacctggttc cactggattt gtgctccgtc
     1681 cctggtcact tccaccgcga ggccgctcgg tggaggaaga atcattcgat tgagcgccgg
     1741 gtttgatttt ccaaacactg gtcccgtaaa ttttccagtc tgatcttcga agagggcagc
     1801 aacagtatag ccagtccacg aacctgattt cgctcgttcg gattcatctg tgtactgatt
     1861 cgatgttgtc ttcgtgctgc cagtgtattt ctgtttcgta gcgtcataga agtgaatgat
     1921 gtagccgttt gccccaggga ccggatccca ttttaccgtt atgcgatcac ggtacaaccc
     1981 gcgagatgca tcgagcctgg ttaccccgtt tcctgccatg atcggagcgc tccaggcgcc
     2041 ggatgtcagg cgagatctgg ctcgaataga gtatactgcg tccattcgtc cagctggatc
     2101 acgaaacgta gtttcgcgcg aaactgccag gagttgaaac gatttgagtg ctggctcata
     2161 gcgcgcaatg tcgtaccact ctgcgccgga agtctgattc caggagagag tgatgccatt
     2221 ggtagatgca gccgcaggtg tctgcgtcat ttgggatggg agtgcatccg gagtcaccaa
     2281 agaccgaaag gcagtgccca gggagttcgc cacaatatcg aggacgggtg gaagaacata
     2341 acgttcgaag atgccagggt tgcgcgcgaa gccttctgca gttggactca gttcaggatc
     2401 ggatctgcct gtgcgataga tggcaatgat cctatatttg taaggaatac ctttttctac
     2461 gttgttgtcc gtgaaacgag gtgccacaga aaacccgatt ctctcaaatc gcatagaaga
     2521 tcgttgtatt tcataggcga ctgcgccatc cgcactcgtc caggtgagtt ccactttatc
     2581 ttcgaagacc cctcgcgttg cggccacttc gcgcggagca agaaggagat gttcgagaga
     2641 aggcggactg gcgctagcct tgtctctttg ttcaattacg aatcctgcag tgacaatttt
     2701 aggaaacact ctgtagctta tgtaagcgaa tccgttttct ccccacgctc taccccatga
     2761 gttcattact ttgaaagcac cccgcccgcc aggtgtgtct ttgctatcat cgtatcctac
     2821 gatggtcacc gcatggcctc cgaagaactt tcctcggaag tcatcgtata caggatttga
     2881 tttatctaat ttataaaaat tctcatatgc cagaattcca agcatcacag ggcgaccttt
     2941 tgataattct gtgcgaatcg aatctacagc cgctgaatcg atcgcgcgaa aaccgcgtga
     3001 cctgaattgc attgcctccg cgagagcttc gggccccggt tgcgaacgat agtcgcccag
     3061 actgtacggc attctcttcc aggtggacac tcctcgcgtt acgagaaggc gaagcgccgc
     3121 gattggatca gaaccctggt ctctcccgcc gttgatttga ttgtaaatga aagacgggga
     3181 catgacgcgt gttccctggc ctccgtaaat gggagaatca tagctccaac cgaatgcttt
     3241 cttttcctga taagatcgaa tcgcgtacgc cagagaccat ccaaggcagc taccctgatc
     3301 accctggaat cctacaggtg gcatatctgc cgacatatcc acggaagaag gaagacctct
     3361 ctctttgtag aaagcactgt ccaccatggg tagacttctg tattcatccg gatttggagg
     3421 gaggaaaccg caatgatatg aaccgtttct gcattctgca ggcatgacgc tggcagggtt
     3481 gaatgattcc tgagcgccta acgttgaaaa agaaagtacg gcgatcgtga agactgctcc
     3541 cggagcagcc ttgcgaaagc cggtcatcac ctttgtgctg ttattcgtag actggagtga
     3601 cccattgttt gaattcactg tcctctccga gaacacactt gaggaatctg ctgcgaagtt
     3661 tattggtaac aggtccgact tccgtgccaa tgactctgtg atcaatggag gatacccagg
     3721 cgatttgcgc gccggtaccc gtgaagaaga cttcatctgc gacataaagc tctgcgcgag
     3781 caatgtctcg ttcttccacc gggatgcctt ctgatttcgc aagttccagc acgctgcggc
     3841 gtgtgacacc ttcaaggacg gaggcggcaa cgggcggggt gacgagtact ccatcgcgca
     3901 ccatgaatat gttttcagcg gacccttcgc agacaaatcc gcgtaagtcc aggaagattg
     3961 cttcgtcgta tccattttcc acggcctccg acttggccag gccggagttt gcgtaaccgc
     4021 cggaaacctt gccacgtgtg ggtatgatgt tatcgtcaat gcgcctccaa cttgagacgc
     4081 atgcggacag ccctttgttc gtctcaagat agtcgtccag cctcaaggaa tatagagaaa
     4141 actcatcttt tacgtcgtgg aaccgagggg acagctgctc ggctgccttg tacaaaaacg
     4201 ggcggaaata cacattctgt ctgtaattgt tgaatctggc tacctggatg aggatgttgg
     4261 ccatttcctt ttctgaaaga ggtggagtca tccgcatcat tttcgcgctc tggtgcagac
     4321 gtttgaaatg tctgtcgatt ctgaaaatca gcagattgtc cattcctttg gaatagtaac
     4381 cgcgcactcc gccaaagacg gctgtaccgt attgcagggc atgtgtggcc acattcacgg
     4441 tgcaatcgga tagctttttg aattcactct tgtggtaggc gattgtgctg agatccatgg
     4501 gcagaggctc ggagaccgcg ttcaggcgtc aaccgtggtt tttgggcaac gggccatcgc
     4561 tcctcagcgg ttacttgcct gattgctttc atcgagtgaa aattcgtcca tgatctgtcc
     4621 cagctggtct gctctggttc tcatgtttcc ggaaacttcc tgcaacgctt ctgattcatg
     4681 caagatcttg tctgcttctg acgcgtttgt acgagtcgat tccgcaagtt cgcgtgccgc
     4741 gtttttactc tctttggccg ctccttctac ggtggaagct gcgttcgaga gttgttgcat
     4801 ggatgctgca atcgcgctca ccctcgatac ttgttcgtcc actcgcgttg caaagccgct
     4861 gacagaatct cccatctgaa gcaagtcgcg catcatcact ccaaagctat ctgcggctgc
     4921 gagcacctga gtgtggcttt ccagaatcgc actttgaatg gaggaggtgt ttgtttctac
     4981 ttctttgatt ctggccatgg ttcgatctgc catgcgcgaa acttcttctg caactacggc
     5041 gaatcctcga ccagcttcac ctgctctcgc agcttcaatg gatgcattga gtgcgagcag
     5101 gttgatgcgg tctgacaatt catgcatgag agacatgatc gcaccgattt tctgactgcc
     5161 atctttcact tgttccatgg attcagacac aatctgaatt tgttgtaagc cagagcgcgc
     5221 gcgattggac gattccaggt ttcgtgcgcc aagcgtttgc atggacatcc tcatttcttc
     5281 cgattcagtc gccagctgat gaatggaatg atcgacctct tttgagttct gggccacgac
     5341 agtcatggaa tcggaaacat gatctgagat cgcagatagt tcttctaccg ccgccgctga
     5401 ttcttccgaa ctcgccgcca gattttgcga agccttcatc aatttcttag cagtacccga
     5461 gatttcgaaa gctgcccctc tggtctggtg aacttgatcg cgcacactta gaagcatggc
     5521 tgcaatcttt cgcgaacttc tttttaccgc agagagaatt gtgctgcgtt catctgagcc
     5581 agagtcatct atttctagat tgagttttcc ttccgaaatc tgttgaagag ttccggtgac
     5641 cgcaccaaga gtgtttgaaa cgctgcgcac cgccatccag gaaaaaagaa acgtcagcac
     5701 ggttacgatg aggcctgcac tgcccagaga aatccagaac agtcttagat ccctctcctg
     5761 gtttcctttt gactgattag aaatgagttc tacaagtgcg ctgagttgtt gatccgttgg
     5821 tctatccatt cctttcacac gacgatccgc ggcgcgcgta aaatctggac taccagcgga
     5881 aagttcctcc agtgtcactt cgtattgctt gagcaagtcg gaatgccttt tctgtatctc
     5941 gattgctttt gatttcgtgt ccgaattcgt ttcgctctct attagttttt gaagaagggc
     6001 ctggaacgca gtggattcgg tgcgaaatgc gatcagatgc ttcttgtatg cttccggatc
     6061 cgatccgcgg atgagtatgt ttttccattc ctgaacttgg agtttgaaat ggacctggct
     6121 ttgtctcacc agttccatgc gcgctacagc atcattcacg cgcgaggaca gggccatctc
     6181 gcttacggct acgaaaacgc ctgcgaacaa gaaggtcgca aagaagccaa gtgccagcat
     6241 ccagattcga gcacggatgc tcagccgcga aataagcatg gccaatgact ctcatgcatg
     6301 cgaaagtggc aatcgttttc gcacatgtgt cgcacccgtg tcgcacatcc ttcgcacgcc
     6361 gttcgcgcac tcgttgtacc tgaagccatg gtttcgcgcc gccgttaggc gatattgcga
     6421 cagaatctca ggcccaacct cttgccacaa tcgcatcgcg caatctttct acggaaatca
     6481 agtaggcggc cgtgcgaaga tccgccccgt gagctctcgc tgacttctgt accgcgtggt
     6541 atgcatcgat catccttttt tccagtctgg acgcgacttc agtctcttcc catgcggaat
     6601 gagtcacgtt ctgaacccat tcgaaatacg aaacggtgac tcctcctgcg ttcgccagta
     6661 tatcaggaat cacctgaacg ccgcgcgaag agagaatttt atctgcctcg ggggtggttg
     6721 gaccgttagc cgcttctatt acggccttgc attgaatgtg atgcgcgttt tcggatgtga
     6781 tggaattttc gagagcggct ggtatgagaa catcaccttc acattgcaac gcgagtttac
     6841 tgtcgatcat gaaccctgaa ccgtacaggt gcaatcctcc ggcgtctttc gcttcgcaag
     6901 ctttttgaat gttgagtccc gtcggatcct gtagaccagt tttggagtca gagatggcta
     6961 cgattttaca ctccagttcg cttagaaggg tggctgccgt tcttccggca ttgccaaatc
     7021 cctggatcac aattcgtgcg ttcgcaggtt cgatcttccg gtcgcgcagg tattctcgca
     7081 caatgtagag acatccctgt gcggtagcac gatcgcgccc ttttgaacct cctagaacga
     7141 ttggttttcc agtgatggca gagggcacgt tgtatccgcg caatctgctg agcgtgtcca
     7201 tcatccagcc catgatttca ggggtggtgt aaacgtcagg tgccgggatg tctatttccg
     7261 gtccaagtat cacagacgca gcttccgcat acgcacgact cagtctctcc agctccccgt
     7321 gcgaaagtga gcgaggatcg catacgactc cgcctttacc gccaccaaat ggaagatcta
     7381 ccacgccgca tttgaatgtc atccacatgg acagagcttt gacttcatcg agcgtgacgt
     7441 ttggatgaaa tcgaattcct cctttcgcgg gcccaagtgc atccgtgtgt tgcgcgcgaa
     7501 aaccttcaaa gacgcggacg cttccgtcgt ccatgcgcac tggaaaattc atgatcaaca
     7561 ctctacgagg tcgcaatatg aattcagtga cgctcctggg aagttttaga atttcggcgg
     7621 ctctggatat ctgtttgcat gcaatcaaat acgggttcag ttcgtctgcg gattcggcca
     7681 tatctgcccg gtttatcttc gccttgttga atcacaattt ttttctcgtc tgtagcgcaa
     7741 gcgttcgcac aaatgcgcca tgtcgattca tctcttgaag agaaggattc cgcgaggcgg
     7801 agaattgctt ccagtcattg gtctgggcac ctggcagagt tttgacaaag agccttccga
     7861 ggaactctca aaagtgctcg caacttttta tgaagggggc gggaagctga tcgattcatc
     7921 tcccatgtat ggaaagtcag aagaaacggt gggctctctc attgccacgc gtcgcaaaga
     7981 tttctttgtt gcgaccaagg tatggacacg cggagaaaaa caaggatttg cacaaatgga
     8041 ggaatcgttt cgcaaattgc aaacgcgcaa ggtggatttg atgcagattc acaacctggt
     8101 ggactggcgc attcatttac atactcttcg catgatgaaa gagcagggca gaattcgata
     8161 cattggagtc acgcactacg ctccgtcttc tttcggagag cttacactcg ccatcaggga
     8221 tcgggctgtt gattttgttc agctacctta ttctgcgttc acacgcacag cggaagatgg
     8281 aatccttgcg gaggcagcgg atcgcggtgt ggccgtgatc gtgaatcgtc cgttcgaaga
     8341 aggtgccttg ctcagacgat tgcgcgcgaa agctctgcca acagtggcag ctgagcttgg
     8401 atgtttttcc tgggctgagc ttctattgaa attcattctg gccagaccag aagtaacgtg
     8461 cgtcgttcct gccacatcca acccggtaca catgcgcgaa atcttgcgtg cgggcaccgg
     8521 tcgtttgccg gatgagcgcg aaagagaatc gattgcgatg gccgctcgag gttgatgtct
     8581 attgccaggg agacggagtg gctcgcacgc gatgaaatcg aatggagttt gtttccttcc
     8641 cttcgcccag cggcagtgca gccacaaaga ctactgtttc tccaggtgta gcaaagccgt
     8701 gctcgactag atagcgatcc gcttccgcaa tttgctcatc catttcagaa ttgtctcgaa
     8761 tggaaacagg cgttacacct cgcagtacat tcagccttcg cagagtggga agcccggatg
     8821 ccagcgcaag aattgggatt ttagatctcc atttggaaag cagaagggcc gttaaatttg
     8881 aacccgcaag tactatgatc gctcgcgctc gcaacctttc cgccgcactg acagctgctc
     8941 cggcggctcc cgcagaaaag gaaaccatgt ccgcttcaaa gcttggtttt tctatgttct
     9001 ggtgttctcc cgcttccttt gtaattcgaa gcattgcttc tactacaaga gatgggtgtt
     9061 gacccacagc cgtttcctca gacagcatga cagcatctgt tccgtctaac accgcgttgg
     9121 cgacgtccgt tacttctgcg cgcgatggac gatttgcacg aaccatggat cgcagcattt
     9181 ctgtagctac aatggcccac ttgccgcgct gagctgcgcg atgaagaatt tctttttgaa
     9241 tgaagggcac tctttcgaat tcaacctcca ctcccagatc tcccctggca atcatgatgc
     9301 catctgccgc gcgaaggatg tcttcaatat tttgaagtgc ttccgggcgt tccagttttg
     9361 ccatgatggg aattctgctt gatcccagac attcccgcgc tttgtgaatg tcttctgctc
     9421 ttcgcacgaa ggaaatggcc accacatcaa tgtccgtggc agcaatgtag gctagatcct
     9481 ttctatcttt ttccgtaaga gtttccacac tgaggtgagt tccaggcagg tttaagcctt
     9541 tgtttgagaa gagctggcct ccgcgaacaa tgcgcgcgtt tacatctgtg gatgttacgt
     9601 tttccacaag tagttcaatg gtcccgtctg cgaggagcaa tctcgaaccg ggcttcacgt
     9661 ctctggtaag gaattcatag gggctatgaa cgatttgatc tgtgcctgtg atttcgcgcg
     9721 ttgtaatggt gaatgttttt ccttctacaa ggaggactcc ttcgtctgcg agctttccaa
     9781 tgcgcagctt tggaccttga atatccgcca gaattgcaat gggcacacct tctgactgag
     9841 agaccgctcg cacgcgatca atgagcggtt gcatggcagg gccatccgcg tgagaaaaat
     9901 taagccgaaa gcaatccgca cccgcgcgga tgagagagcg aatggcatct tcggaagagg
     9961 aagccggtcc aagagttgcg atgatctttg ttaattttgc acgattcata agaattcttt
    10021 tcgaacactc ctttatttca caatcgtaat ctggcgggcg gctgactaca ttcttagcac
    10081 aatctttcct ttcacatgcc ccgcttcgct cagtgcgtga gcttctcgcg ctcgcgaaag
    10141 cggaaagatc gtgctgatca caggcttgat ggtctttcct tgtatgtaat gccggagcat
    10201 gtccagatca tcactggatt cttttgtaat gatgaagctg caagtaggtc ctccaaaaaa
    10261 tccgcctctt agagaatccc agaaaacgcc tggaccaagt tcgaccgtta tgtactttcc
    10321 atcaggctta agaatatcca cgcagtctgc ataatacgtt tttccaacgc aatcaaaaac
    10381 tatgtcatac ttactatcga ttcgcgcaaa gttttcgcgc gtgtagtcaa tgaatctgtt
    10441 cgcgccagcg gatcgcacaa tctcttcgct tgagccactg gccactgccg ttacatccgc
    10501 ctggaaaatg cgcgcgatct gaatggcgaa agttcccact ccgccggcag cgccatagat
    10561 cagtaccttc tggctgtctt gaatttctcc tggatcgcga agagcctgaa gcgctgtcaa
    10621 accggcaaga gggacagcgg cgcattcttc gtaagtcagg tttcgcggag cctgcgccgc
    10681 cagtctctcc gggacagctg caagggtcgc gaaagcgcct cctttcatgg gcggaagcat
    10741 cccgaagact ctatcgcctt tctgaaatcg tcttgtgttc ttgcctgtgg aaagtacttc
    10801 cccggcaaaa tcagatcccg ggatccgtgg aaaatttccg ccgctgagaa gcctgagcgc
    10861 tcctgatcgg atcttccagt cgattggatt gactgacact gcgtgaatcc ggacaagcag
    10921 gtcatcctct cccatgacag gatccggtag atccatctct tccaggactt ctgccttgcc
    10981 gtatttcttg tacgcaatgg cgcgcatttt cagatttcgt ctctccctct aattccggtc
    11041 aattcgttct ccactcgttt tgatttttag ttggattttt tcccgttatc cggcaatgag
    11101 acagcatgct cgtgcgattt ttctttctgg gcctgctctt gaattcaagc tgcgcccttc
    11161 ttgttcgcaa gggttcggtg gcggagatcc aacagattgc agtgcagggg caggtgcgac
    11221 gctacatttt gcatgtgcct gcttcctacg atgcgcagaa gaagactgct cttgtggttg
    11281 tgcttcatgg cggcggcgga aacggacgaa acgcggagaa aatgagcggt ttcagcgcga
    11341 aggcggacaa agaaggcttc attgtggtct atccatacgg aagcggacgc ctcgatgaaa
    11401 ggctgctcac ctggaatgca ggaaactgct gtggatacgc gctcgacaag aatgtggatg
    11461 atgttgcgtt cattcgcgag cttgtcgttt tgttaaagag acagtactcg attgatgaca
    11521 aacagatctt cgctaccggc atgtccaacg gtggcatgat gactcatcgc cttgcgtgcg
    11581 aaatgtcgga cgttttttct ggagttgctc cagtttctgg ggcgatgaac gttcctgtgt
    11641 gcaatccagc gcgtgcggtt tctatggtga tctttcatgg caaaaaggat gagcatgtat
    11701 tgtttgaggg gggcgctcca aaaaaaagtg tggattcgca tcctcgcgtg gacaaatccg
    11761 tggctgctgc ggtagaattt tggaagaaca ggaatggctg tgcgcaatcg ccgactgaat
    11821 cgcatggaaa gattcagata gaagattttt cctgctcgca ggcggggctg gcagtatatg
    11881 cgatcgaaga cgaagggcat acctggcccg gtggtgagaa aggctacttt gctgcggatg
    11941 aaccaacgcg tgaactatct gccacagacg ttatgtggga tttctggcag aagaattcgc
    12001 gtccgtaatg gagggtgaag cgtcgaactt gcgcgtcagg cgaacttcat ttcctggaga
    12061 agtataattc acagaatcaa acatggctcg cgttaaaaca attcctcttc catgctttcg
    12121 tttgatctcc gtgctgctct tgagattgcg ctccatcaaa gccctttcag tctctcctct
    12181 tcattctgcg ggatcggagc tgaaatcata cctcacctct tattgaaatg cttttcaatt
    12241 cttgtgtccg gcacgatcca cataatcgcc acgatcacga tcactgccat ggcagcctgt
    12301 gagttataga atgcaatcgc aatggagaga atataaagca ccagagaagc aattcctttg
    12361 cggtcgtttc ctacagcctt tgcgagagct gagtcttttc cctctgcggc gatcagcatc
    12421 tgttctagaa tatagtaagc tgctccgcac ataagcagaa cgaatgcgta agcgacaacc
    12481 gtattggttt caaaatgatt ttctccgctc caggctgtca caaaagggat gagcgaaagc
    12541 cagaagagaa gatgcatgtt ggcccatagt actttgccgt tgattctatt cgcaaggtgc
    12601 agcatgtgat ggtgattgtt ccagtagatt ccaaggtaaa gaaagctcag gacgtagctc
    12661 aggaatacag gtacaagtgg aagtagctgc gtcagatcgc tgccgtgcgg caccttcagt
    12721 tccagtacca taatggtgat gagaatggcg attacgccgt cgctaaatgc ttctagtctg
    12781 tttctggtca tggcactcgg tgcggttctg agcgcatgtg tcaaactatt gatcagactg
    12841 cggctagttt ttgctctctt tctgctcgaa cgagtgcccg caaaagctct tcaatatctc
    12901 catcgagcat agcggacagg ttatgcgatg tgaacccgat gcgatgatct gtcaggcgat
    12961 tctgcggaaa gttgtatgtg cgaatctttt ctgacctgtc tccgctgcca acctggtctt
    13021 tcttgagtgc cgcttcttcc gcatgcctgg acgatttttg cctctcaagc agtcgcgcac
    13081 gtagcacttt cattgccttg cttttgttct tcagttgcga tctttcgtcc tgacatgtga
    13141 tcacgagtcc gctaggaatg tgcgtgatgc gaatggcaga atctgttttg ttcacatgct
    13201 gcccgcctgc accgctcgcg cgatacgtat caatctgaag gtctttgtca tcgattgaga
    13261 tttcttcttc ttccgcttct gcaagcacgg cgacagtgac cgcactcgta tgaattcgtc
    13321 cgccactttc cgttgttgga attctttgta cccggtgcgt acccgcttcc tgatgaaaca
    13381 ggtcataggc ttcctttcca tctatggaaa atgcaatctc tttatatccg ccaaggtctg
    13441 actcaacggt agaaagcact tcgagcttca tcgcggacct ttccacataa cgcatgtaca
    13501 tgcgaaatag atcgtctgcg aaaagggccg cttcttctcc gcctgttccg gcgcgaattt
    13561 caactatgac tgatttgccg gagtttggat ccggcggaag aagaagcgct tccatttcat
    13621 ctctgccggc cgcaagttct tgatccagcc gaaccttctc ttccgatgcc atatcgcgca
    13681 agtcctgatc tgcggttttg tccgaaagga tgcgcgacgc ttcatcttga tctttaagaa
    13741 tttttaggaa tcgttcaatg ggctccaccc tggcagaaag gatggaatgc tcgcgtgcca
    13801 gttccttgaa tcgagccgga ttggatgtta cgtctggctg cgaaaggaga tcttcgatct
    13861 cgcgcactcg cagtagttct ttttcaagtc tgtctttgac ggcgctcatt ttgaaaatta
    13921 cccccgacag gagtcgaacc tgtccacata gtttaggaaa ctactgctct atccacctga
    13981 gctacggggg cctcgtgtca gagtggtcga aaaaaggcct gagtcaacct gatttgtgat
    14041 tgggttcttc cgcatgcatg cagcaatgcg gcgtgagctt gctctttcat tttttgcaca
    14101 tcgcggtcat ggcagcgaat ctattcgcat ggaccattcc caggctgcgt cggtttcatt
    14161 tgatcgtggt gggatgtact ctcttttcct ggtgtgttct gggacttcgc tacgggtttg
    14221 gttattgttt tctgacggac tggcactggt ccgtccttga gtcacaggga gtgcgaaatt
    14281 tgcccggttc ttacgtgaag tatttatttg atttgatcac cggatgggac tcaagtccct
    14341 tctggatcga cgtggctaca ggagtcagct tttccattgc gatctcgagt tcactgtatg
    14401 tgaataggga tttgttcaga ttcttgcgtc ctacgtcata gaacccacgg aaagaagatc
    14461 caggtgcgcg atttgtattt cgcatactct tctccaaatt cttcctccag cattttctct
    14521 tctgccggta tgcgaatcca gcatagaaag aggaaagaca caagagacgg aatcagcaca
    14581 aacaagtttg cagtgagcag tccttgcgac agaatcgaaa caataatggc actgtacatg
    14641 ggatgacgga ctgtcctgaa cggtccggtc tcgatgattg tgtgctcgtg ttcgatttcg
    14701 agcacggggg accaatgttt tcccagaaaa gaatgcactc tccagaaata cacaatactg
    14761 gcgaacatga tgcatccccc ggcgattctc gcaatggtgt ggaggtgaaa tgaaccgttc
    14821 tctagaaagt tggtaaatac atataatcca gtggatcccc accagcacac cgcaaccaga
    14881 gtcaccagaa aggattcgcg caagaagtgc acgcggcgct tcttgtgttt ctggttgtag
    14941 cggtattgat agatgctgcg a
//
LOCUS       DAORFF010000015        14873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1716_length_14873_cov_3.943650, whole genome shotgun sequence.
ACCESSION   DAORFF010000015 DAORFF010000000
VERSION     DAORFF010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14873
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1716_length_14873_cov_3.943650"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PKK76_01335"
     CDS             <1..505
                     /locus_tag="PKK76_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNL67129.1"
                     /translation="TVRHMPFPVLAAVNGHAIGAALAITLACDLRYFVPDAKYSLNFV
                     RIGIHPGMGSSFLLKQIAGMMRAQELILTGRYFSGTEAEQMGICHGTFPADTIVEKVT
                     EIAREIALGAPRAVRLAKRSLYEATDIENAIQLESHFQAETYQTQDFREALKAIEEKR
                     TPTFKDE"
     gene            511..1515
                     /gene="mltG"
                     /locus_tag="PKK76_01340"
     CDS             511..1515
                     /gene="mltG"
                     /locus_tag="PKK76_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNL67130.1"
                     /translation="MDKKKIIRIIIGAAAALLIGAGLFAIAGPGRPPGNGSKKVLFEI
                     ESGSGPGSVSERLAAEGLITNSAYLRFLLRTTAGGKIKMGVYEINDGMSAGEIASLIS
                     RGKVKMISITIPEGWHNRQIADEMVAKGLVKTTEEFLKLTEDPQVLAKYKIPAKTTEG
                     YLFPETYSVPLGYSATSFQDLMIRQFLKKAREAGAAENITPQELHEKVIMASIIEREA
                     LKREELPLMAQVFENRLEKRMKLESCATIQYLFERPHKKLYERDLVIQSPYNTYIHKG
                     LPPGPISNPGLPALRAAFHPEATDALFFVLKPDGSHHFSKTFKEHNEAKQKYLGGAPV
                     "
     gene            1512..1973
                     /locus_tag="PKK76_01345"
     CDS             1512..1973
                     /locus_tag="PKK76_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67131.1"
                     /translation="MKDVADAANSLKNMLSQLEKENKKLTGDLEVKRKERATRIKEAK
                     ATQKKMHSHLEKIDGHLAKGRAALDKQTGALAGIKKIVKQQEQTLKDLHLRLERLEHG
                     LRGGKMSIPDARRELMKMLEDLQGMKKQREKIMSSEGKVNATNFPSFRLDP"
     gene            2001..2441
                     /gene="rplM"
                     /locus_tag="PKK76_01350"
     CDS             2001..2441
                     /gene="rplM"
                     /locus_tag="PKK76_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNL67132.1"
                     /translation="MIILENQKTKVTKDPEIVKSWVLIDAKDQILGRLASKAAHRLRG
                     KHRVDFSENQDIGDFVVIINAKHIRTTGRKMEDKMYRHHSRHPGGLKEFNLKTILEKH
                     PTLAVEKAVKRMIPSGPLGRKIMGNLKIYAGAEHPHKPQKPTQI"
     gene            2457..2849
                     /gene="rpsI"
                     /locus_tag="PKK76_01355"
     CDS             2457..2849
                     /gene="rpsI"
                     /locus_tag="PKK76_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNL67133.1"
                     /translation="MAVKATLIVGRRKTAVARAKLTAGTGKFIVNGKDLKEFFPLARL
                     QKTAAQPLVLAGLEAKYDVSANVAGGGITGQAGAVRLSLARALQTVNPALHTDLKKAG
                     FLKRDPRMVERKKYGKRKARRGTQFSKR"
     gene            2922..3101
                     /locus_tag="PKK76_01360"
     CDS             2922..3101
                     /locus_tag="PKK76_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67134.1"
                     /translation="MQIESGSPGFEAISRSTRMLNELNQTIVDKTQEFQSKAVKMNVA
                     EQVTSSAENQIDLQA"
     gene            complement(3104..3556)
                     /locus_tag="PKK76_01365"
     CDS             complement(3104..3556)
                     /locus_tag="PKK76_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67135.1"
                     /translation="MQTFLISGTLIARGPFDFSHYSTLREGTRFKIQNGGEWIGKGQI
                     VSISRNQIRMVFEIHKSGVSVRGDVSLSHIRTEKQFEILALNYSGNVNGAPENSSEQV
                     KSDLFLCENGILAFRFLKGSRFFQLSVNRAGESSVTTDWGTGKLLPER"
     gene            complement(3603..5429)
                     /locus_tag="PKK76_01370"
     CDS             complement(3603..5429)
                     /locus_tag="PKK76_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67136.1"
                     /translation="MKFRPFPQILAFLILTALSADSSSLVIGTSYFAFSPNGDGIQDE
                     IEVRFLEIPSSLETPADYALRIENDRSLVRTIRADLRWIRPRRAVSNLFLPFAETVRP
                     LSFPESIPWDGRDDAGKILPDGNYRITVTAADASGRVSNSNSVTVELSTRRPQLTLSA
                     DVRAIVRGVTQDGKPADSTKGEFAISQSAQADPKTVFTGRIVNAAGEILETRTWPGTL
                     SPKIIWNGRTAKGDLVPWGCYTYWLEARSPAGVVVRESIPGLLVVTELPQIDLQPDES
                     FSPNGDSVKDKIQFLIQYFNQSLRAGVKSYRLQILTHDSPEIIYDTQPQRVLPGILVW
                     DGKNAAGAIVKDGIYDAKLTLETTQGTVSSAPRSFRVDTSPPGVSFSSSSSSFSPDGD
                     GEEEDISFKMDAADISGIEQWTIRVLVTPDTQNRFQKLIRTWQGAALPERIMWNGLSD
                     AGEPIESLETFTVVLEARDRAGNLSKPEKLVVRSKTLLRPVAPGQRALYARIPVQKYF
                     NADSTLTSQGEAAISDVLSHLSRFRKYNIHLYVNSGLPGGEEQNLEITERRALQLYKR
                     MQGSWKDRMDFQGLGESEILNPSPDDFSQYRNERIELQLYVP"
     gene            5480..6622
                     /gene="mnmA"
                     /locus_tag="PKK76_01375"
     CDS             5480..6622
                     /gene="mnmA"
                     /locus_tag="PKK76_01375"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNL67137.1"
                     /translation="MAEKVLVAMSGGVDSAVAALLLIEQGYEVVGVNLRTWDYEAVAC
                     DTRKKSCCSPRDVQDARDTGIHLDIPFYVVRIEKAFKESVMDRFVLDYVSGRTPNPCV
                     ECNTFVKFGILFEKARSLGIDRIATGHYARTHAGKRHSIRVGRDNRKNQAYYLYGLSQ
                     DAVANSIFPLGDMEKPAVREIARRHDLVVAEKEESQEICFVPENDYRSFLKRQGVEFQ
                     PGYFKDRDGRILGKHSGKENYTVGQRKGLGISWQKPLYVMSIETNGDVILGTDEDDFM
                     REFYVEQTIYQALGPEDFSGEIHARVQVRYRANPVSCVVSRAGSSDKIAWNGKPEGDI
                     LRVRLLEGAGAVTPGQSAVFFPSVELANAHDLPEDVVLAGGVIQKT"
     gene            complement(6643..7134)
                     /locus_tag="PKK76_01380"
     CDS             complement(6643..7134)
                     /locus_tag="PKK76_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67138.1"
                     /translation="MRIIAPLFLVIPFMVVLHCSSGMARTHYPALSGSWNVDLDWTIQ
                     DENFKRVAKTSPDAMQKARFLSRITSMTFGEERYSATIDFRVLSAEERVAGGVPNRAI
                     EDSVAIMDSRLENGVWKLTILGNKSHRLEVKYVKVLSEDHLVFTASPVGPEDFPLHLR
                     RKN"
     assembly_gap    7317..7416
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7718..8302)
                     /locus_tag="PKK76_01385"
     CDS             complement(7718..8302)
                     /locus_tag="PKK76_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNL67139.1"
                     /translation="MSISEQQTQQNEIMMFFRYLTNANFSCASRGFCYFYVTQAAQAS
                     ANIGGIATGDSMCSADPARPTAATFTVKAFLVGSTVRRASVSASVGDGQIDWVLLANQ
                     EYRQANGTTVIGITGANRLFSFPLSAGFVNSGISYKTGMDTAWRNSGNDCGAWSNTAG
                     NQDLGNGNVTSNMSIFGGTIACTNPGYYLLCVEQ"
     gene            complement(8378..9904)
                     /locus_tag="PKK76_01390"
     CDS             complement(8378..9904)
                     /locus_tag="PKK76_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNL67140.1"
                     /translation="MSRKPSRSRKIALRAPGWKELIILRLCASNNIMRLIIILFLSLV
                     SSLSAERLIEDHFLISMQSQAMGTYTRIMDRDESTGEIETRVEMQIRIKRFKEPVVIS
                     RTSIVREDKSGRPIAFDIISNESQIPAKTHGMRVGDEFVVTTESAGKIKETRVAAPEE
                     AIGPRAADLLQQQKGFSPGTSYEYEVFSPDSADDLVNDYNVEVIGTSKFKTPRGEVDV
                     SVVKSHPLPGGPAETSLVGKDGTIYSLELESAGFKMTRATREEAAATQFNADVADLFF
                     IPVEGSIDRPRASKQSRFEISSSKGVPMIASVAYQQSEKKGDKLIVTQKVPLLPRGAR
                     APAPDAKDPNRLPDRYVNSDDPEIIKLAAKIVGKETDISRKVMLMNSWVFRHIEKKNF
                     SVGYASASEVVRNLEGDCTEHSVLFAALARSQGIPTRLALGVVYMEMEGKPSFLFHQW
                     NEVYVGGQWIPVDPTFGEAPADASRVLIGYNSESRESSQRPGIDWAGQVRIRILGPGE
                     "
     gene            9917..11857
                     /locus_tag="PKK76_01395"
     CDS             9917..11857
                     /locus_tag="PKK76_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004494734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNL67141.1"
                     /translation="MEKESLYALVRDTVATYAERPCYWVKPDPRAEVFSAVSYFNWRA
                     DMKRFSAYLIHKLSCEPGTKVGLLCDNRYEWNLISMGITTIGCIDVPRGCDATHQDIN
                     YILNHTECPVLVVEHEKMLKTVVGMLGELPSLKHIISIEGPEKFKNLDEIASKLGKTQ
                     LHFLVDTFAEGEEILAKLGEGFLKKRGEEIRPHSIATIIYTSGTTGSPKGVMLEHRSF
                     CWEVNQMQQVLPLGERDRTVIFLPPWHIAERLLETTLIACGSSMANASIISLAADMGV
                     IKPTALVSVPRVWEQLYRRVFDNVRKQPEKTQNIFNFALNAAQTYTDIMDTLLDRFAE
                     TEDEEKTSTLTRKAIAAAMLPVFAILNIPAQIILKKVKNIFGGRLKFAVSGAGALPEH
                     VAVFFRAVGIPILDGYGMTETTGVAAISALPFPRRGCVGRPIPGAQIQLRDDTGKVIT
                     RPGVKGVCWHKGPHVMRGYYKADDKTAEIMRDGWLNSGDIFVWTNTGEIKFAGRAKDT
                     IVLAGGENVEPGPIEMKLLEHPFISQVVVVGQDKKTLGALIVPNYDRCKEEFKNMGFE
                     APSDYAQWDTHKEIHKFFQDIVKQQVSGSHGFKSFEKVTGFHIMRKEFEKGKEMTETM
                     KIKRNVVFDLYKKEIDSLYRDD"
     gene            complement(11886..12275)
                     /locus_tag="PKK76_01400"
     CDS             complement(11886..12275)
                     /locus_tag="PKK76_01400"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNL67142.1"
                     /translation="MLEPGLASGILEIRSPKRRSLTMHDHRSVITHSMAISGDLTVDT
                     HLIIEGAISATKIDSKNHTVVVGQYSAVHGDVYGSQILVLGEINGNLFASEKIDIRST
                     AVINGDVNAPLIKMEEKSRLNGRLNYT"
     gene            complement(12379..13146)
                     /locus_tag="PKK76_01405"
     CDS             complement(12379..13146)
                     /locus_tag="PKK76_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNL67143.1"
                     /translation="MSFVNVQTNGPLSILTLKRPEAKNALNREVLRDIREAVSKIAQD
                     PTQRVMILTGDGAAFCAGADIAEMKGLSAEQAEEFARNGHDTMNAVAASDLISIAAIN
                     GFALGGGLELALACDIRIASSTAKMGLPEVGLGLIPGFGGTQRLSRLIGRARALEMVC
                     SGEIITAEQAASVGLVQKVSAPESLMADANALAEKFLKGKAPSAQKTARWLVISGMEL
                     PLSAGIEREINAFAELFNTKEPVEGLSAFLEKRSPNF"
     gene            13236..14033
                     /locus_tag="PKK76_01410"
     CDS             13236..14033
                     /locus_tag="PKK76_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HNL67144.1"
                     /translation="MRQRVFFEDLRLELENLSSHGLTALKTGTEVEDMSFEEIRILRA
                     LSRDICPLFVKIGGAEARNDIRELAEIEVDAIIAPMIESVYALEKFIESMRDVLSPKA
                     YRRIEKGFNLETATAFNRMTELLTSEAAQELVQVTAARTDLSGSLRVSPEHEIIFELC
                     THIVARCRQYGLRTSVGGAIKSASIEKLVGQIGPDTVNTRHMVMDSAVLAGNPVCVLE
                     KHLMWEVHLYQYLASFPSHKQATHRKRAETLLQRMTPDLALAPVAAV"
     gene            14033..14839
                     /locus_tag="PKK76_01415"
     CDS             14033..14839
                     /locus_tag="PKK76_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67145.1"
                     /translation="MLEAQPVNRTWRDWIRLIPARGADYFLLFYLALDLALILFHDSY
                     RDLLPKGSVTYVRYFDFSLIAIWSIDFILRLRRQEDRWGYVRAHWYEAIGLFPLHPMR
                     YFLALRAAKIGIAYFRILRSDRDVGQLVVEEITFRFRDIFVDTIADAVFLRSLERVDE
                     VMSRLDYETLARSTFQKHQASMQKAVHDSINSKSIIGDLARIPLMGTVVNKIGADVSS
                     IVIEVLETEVTGRIMKDITSSILTEMNERVKLLDLERLTGRRLTTGRTKK"
BASE COUNT         3861 a         3801 c         3654 g         3457 t
ORIGIN      
        1 cacagtgcgt cacatgcctt tcccggttct cgcagcagtc aatggccatg cgattggagc
       61 ggcgctcgcg atcactctgg catgtgacct tcgctatttt gttccggatg cgaagtactc
      121 tttgaatttt gttcgcatag gaatccatcc tggaatggga tcctcttttc ttctcaaaca
      181 aatcgcagga atgatgagag cccaggaact catactcacc ggaagatatt tttccggaac
      241 ggaagcagag cagatgggaa tctgtcacgg aacgtttccg gcagacacca ttgtagagaa
      301 agtcacagag atcgcgcgcg aaatcgcact cggagcaccc agagcagtgc gtcttgccaa
      361 aaggtctctc tacgaagcaa cagacattga aaacgcaatc cagctggaat cgcattttca
      421 ggctgagacg tatcaaactc aggatttcag agaagcactc aaagcaatcg aagaaaaaag
      481 gacacccact ttcaaggacg aataacgttc atggacaaga agaaaatcat ccgaatcatc
      541 attggcgccg ccgccgcact tctcatagga gccggactat tcgccatcgc aggccccggt
      601 cgccccccgg gcaatggctc aaagaaagta ttgtttgaaa tcgaatccgg aagcgggcca
      661 ggcagtgtca gcgaacgcct cgcggcagaa gggctcatca cgaattcagc gtaccttcga
      721 tttctgctgc gaaccacagc cggcggcaag atcaaaatgg gagtctatga aatcaatgac
      781 ggtatgtctg ctggtgaaat cgcttctctg atttctcgtg gcaaagtaaa aatgatttcc
      841 atcaccatcc cggaaggatg gcacaacaga cagatcgcgg atgaaatggt ggccaagggt
      901 ctggtcaaaa cgacggaaga atttctcaag ctgacagaag atccgcaggt tctggcaaaa
      961 tacaaaattc cggcaaagac aacagaaggg tacttgtttc cggaaacata cagtgtgcct
     1021 ctcggatatt ccgcgacatc gtttcaagat ttgatgatcc gccagttctt gaagaaagca
     1081 cgcgaagcgg gagccgcaga aaatatcaca ccgcaagaac tccatgaaaa agtaatcatg
     1141 gcttccatca ttgaaagaga agcgctcaag cgcgaagagc tacctctcat ggcccaggta
     1201 tttgagaatc gcctggaaaa gagaatgaaa ctcgaatcgt gtgccaccat tcaatacttg
     1261 tttgagcgcc ctcacaagaa actatacgaa agagatcttg tgattcaatc gccttacaac
     1321 acgtacatcc acaaaggact tcctcccggg cccatctcaa atccaggact tccggcactt
     1381 cgcgcagctt ttcacccgga agcaacggac gctctcttct tcgttctcaa accggatgga
     1441 tcgcatcact tttccaaaac attcaaagag cacaatgagg ccaaacagaa gtatctgggt
     1501 ggtgctcctg tatgaaagac gtagcagatg cagcgaactc tctaaagaac atgctctctc
     1561 agcttgagaa agagaacaaa aagctgactg gagatctgga agttaagcga aaagaaagag
     1621 cgactcgcat caaagaggca aaggccacgc agaagaagat gcacagtcat ctggaaaaga
     1681 ttgatgggca cctggcaaaa gggcgcgccg cgctcgacaa gcagaccgga gcgctcgcag
     1741 gcatcaaaaa gatagtaaag caacaagagc agacgctcaa agacctccat ctcaggctcg
     1801 aacggttaga acacggactg cgcggaggca aaatgtccat cccggatgcg cgccgtgaac
     1861 tcatgaaaat gctcgaagat ctgcaaggaa tgaagaagca gagagagaag attatgtcct
     1921 cggaaggaaa ggtaaacgca acgaatttcc cctcatttcg ccttgacccc tgaagcgttt
     1981 cggtacgatt ggcactggca atgattattc tcgagaacca gaagacgaaa gtcaccaaag
     2041 acccggaaat cgttaagtcc tgggtcctca ttgacgcaaa agaccagatc ctggggcgtc
     2101 tcgcctccaa ggctgctcat agactgcgcg gaaaacaccg tgttgatttt tcagagaatc
     2161 aagacattgg cgatttcgta gtcatcatca acgcgaagca tattcgcact actggaagaa
     2221 agatggaaga taagatgtat cgccatcact cccgccatcc aggtggactc aaagaattca
     2281 atctcaagac gatcctcgaa aagcatccaa ctctggctgt ggaaaaagcg gtaaaacgca
     2341 tgattccttc cggtcctctt ggacgcaaaa tcatgggcaa cctgaagatt tacgcgggcg
     2401 cagagcatcc acacaaacca caaaaaccaa ctcagatcta atatcaggaa agcatcatgg
     2461 ctgtgaaagc aactctcatt gtaggcagaa gaaagaccgc agtagcgcgc gctaaattga
     2521 cagcgggcac gggtaagttc attgtaaacg gaaaggacct caaagagttc ttcccacttg
     2581 ctcgcctgca aaagacagca gctcagccac tggttctggc cggtcttgaa gccaagtatg
     2641 acgtgtctgc taacgtggcg ggcggcggaa tcacaggcca ggcaggagca gttcgcctca
     2701 gcctcgctcg cgctctgcaa acagtcaatc cagcactcca cacggacctc aagaaggccg
     2761 gattcctgaa gcgcgacccg agaatggtgg aaagaaagaa atacggcaag cgcaaagcac
     2821 gccgcggcac acaattctcc aaacgctgat ttttttcttc agtaatttgc aaaatctgcc
     2881 gatcatgtaa aaggcccaaa tgtgcctgca cggaggcagt catgcaaatt gaatctggat
     2941 cacccggttt cgaagcaatc agtcgttcca cgcgtatgct caacgagctc aatcaaacga
     3001 ttgtagataa gactcaagaa tttcaaagca aagcggttaa gatgaatgtt gcagagcaag
     3061 ttacatcatc cgccgaaaac cagatcgacc tgcaagcata acctcagcgc tcgggcagta
     3121 gctttcccgt tccccagtcc gtggtgacag aggactcgcc tgcccgattt actgaaagct
     3181 ggaagaatct tgagcctttc aaaaagcgaa aggccaggat tccattctca cataaaaaca
     3241 agtccgactt cacctgctca cttgaattct ctggcgcacc attcacgttg ccggaatagt
     3301 tcaaggccag aatttcaaat tgtttttcag ttctgatatg cgaaagagaa acatctccgc
     3361 ggacgctcac gcctgatttg tgaatttcaa acaccatacg tatctggttt cgtgaaatag
     3421 aaacgatctg gcctttacca atccattcgc ctccattttg aatcttaaat cgcgtcccct
     3481 ctcgaagagt tgaatagtgc gaaaaatcaa aggggccacg agcaatcaga gtgcctgaaa
     3541 taagaaaagt ctgcacgatc aaacaaaaag caaccgctcg cctccgccag gatactgcaa
     3601 actcaaggca catacaactg aagctcgatg cgttcgtttc tatactggga aaaatcgtct
     3661 ggagaaggat tgagaatttc tgattctccg agaccctgga aatccattct gtccttccag
     3721 gaaccttgca ttcttttgta caactgcagt gcgcgacgtt ctgtgatttc caggttttgc
     3781 tcttccccac caggaagtcc ggagttcaca tatagatgaa tgttgtattt gcgaaagcgt
     3841 gacagatgcg aaagtacatc gctgatggcc gcttctccct gcgaagtgag agtagagtcc
     3901 gcattgaagt acttttgcac agggattctg gcatagagag ctctctgccc tggtgccaca
     3961 ggacgaagca acgtcttact tcgcacaact agcttttccg gcttagaaag gttaccggct
     4021 cgatcgcggg cctcaagcac cacagtgaaa gtctccaggg attcaatcgg ctcacccgcg
     4081 tccgacaaac cattccacat gattcgctct ggcaaggccg ctccctgcca tgtgcgaatc
     4141 agcttttgaa atcgattctg cgtgtccggt gttactagaa cgcgaatcgt ccattgttct
     4201 ataccggaaa tatccgccgc gtccatctta aaagaaatgt cttcttcttc cccatcgcca
     4261 tccggcgaga agctggaaga actggaagag aacgaaacgc cgggtggtga cgtgtccacc
     4321 cgaaacgatc tgggtgcgct cgatacagtg ccctgagtgg tctccaacgt cagtttcgcg
     4381 tcgtatatcc catctttgac gatcgcccct gccgcgtttt ttccatccca caccagaatt
     4441 ccaggaagga ctctttgagg ttgtgtatcg tatatgattt ccggcgagtc gtgagtcaaa
     4501 atttgaaggc gataggactt cacaccagcc ctgagcgatt gattgaaata ttgaatcaaa
     4561 aactggatct tgtctttcac cgaatcgccg ttaggcgaaa aggattcatc cggctgaagg
     4621 tcgatttgag gaagttctgt aaccacaagg aggccgggga tgctttcgcg tacgactacg
     4681 ccagctggac tgcgcgcttc gagccagtac gtgtagcatc cccacggaac tagatcgcct
     4741 tttgcggttc ttccgttcca gatgatctta ggagaaagag tcccgggcca tgttcgcgtt
     4801 tcgaggattt ccccggccgc gtttacgatc cttccagtaa aaacagtctt tggatccgcc
     4861 tgcgcggatt gcgagatcgc aaattctcct ttcgtagaat cggctggctt tccgtcctga
     4921 gtgactccgc gcacaatcgc gcgaacatcc gccgaaagag taagctgcgg cctgcgagtg
     4981 gaaagttcca ccgtaactga attggaattg ctcacacgcc cgctcgcatc ggctgcagtg
     5041 actgtgattc tgtagttgcc atcgggcagg atcttcccgg catcatccct tccgtcccac
     5101 ggtatgcttt ctggaaagga gagaggacga accgtttctg cgaatggaag aaacaaattg
     5161 gacaccgctc gcctgggacg aatccaccgt aaatccgccc gaatggtgcg aaccagggaa
     5221 cgatcgtttt cgatgcgcaa agcgtaatcg gccggtgttt cgagagagga tgggatttcc
     5281 agaaaccgca cttcgatttc atcctgaatc ccgtccccgt taggtgaaaa tgcgaagtag
     5341 gatgtgccta tgacaaggct cgacgaatcc gccgacagag ccgtcaggat gagaaacgcc
     5401 agaatctgcg gaaaaggtct aaatttcacc gttcaaaagg aagtgccgca cgcccggtct
     5461 ttttgtaaag tggtaaaaca tggcagagaa agtcctggtt gcaatgtcgg gtggcgtgga
     5521 tagcgctgtg gccgcgcttc tgttgattga acagggctac gaagtagtgg gtgtgaatct
     5581 tcgcacgtgg gactacgaag cagttgcctg tgatacaaga aagaagtcat gctgtagccc
     5641 cagagacgtc caggacgcca gagacactgg tattcacctg gacattccat tctatgtagt
     5701 gcgaattgag aaggctttca aagagtccgt gatggaccgt tttgtgctgg attacgtttc
     5761 aggaagaact cccaatccat gcgtagaatg caatacgttc gtcaagtttg gaattctatt
     5821 tgaaaaggcc agatctcttg gcattgatcg aattgcaaca ggtcactatg cgcggaccca
     5881 tgcgggaaaa agacacagca ttcgtgttgg ccgcgacaat agaaaaaatc aagcttacta
     5941 cctctacggg ctttcgcagg atgcggtggc caattccatc ttcccgttag gcgacatgga
     6001 gaaacctgcc gttcgcgaaa tcgctcgcag gcacgatctg gtcgtcgcag agaaagaaga
     6061 gtcgcaggag atatgctttg ttccggaaaa cgactaccgc tcttttctga aaagacaggg
     6121 agttgaattt caacccggct atttcaaaga cagagacgga aggattctgg gaaaacattc
     6181 cggaaaagaa aactacacag taggtcagag aaagggtctc ggaatctcgt ggcaaaagcc
     6241 tctctacgta atgagcattg aaacaaacgg cgacgtcatt cttggaacgg atgaagacga
     6301 tttcatgcgc gaattctacg tagaacagac gatctaccag gcgctcgggc ccgaggattt
     6361 ttccggtgaa attcatgcac gcgtacaagt gcgttatcgt gcgaatcctg tctcctgcgt
     6421 ggtgtcgcgc gctggaagct ctgataagat cgcgtggaat ggaaagccgg aaggcgacat
     6481 actgcgagtg agacttctcg aaggtgcagg tgccgtgact cccggtcaaa gtgcagtgtt
     6541 ctttccgtct gtagaacttg cgaatgccca tgatctcccg gaagatgtag tccttgccgg
     6601 gggagtgatc cagaaaacct gaaggaagtc gatccggtcc ttctagttct tgcggcgcaa
     6661 atgaagagga aagtcttccg ggccgacagg actcgcagta aacacaagat ggtcttcgga
     6721 tagaaccttc acgtatttca cttcgagcct gtgagattta ttccccagaa tcgtaagctt
     6781 ccacactccg ttttccaatc tggaatccat gattgcaacg ctgtcttcaa tggcccggtt
     6841 tggcactccg cctgcgactc tttcttctgc agacaaaacc cgaaaatcaa tcgttgcaga
     6901 atagcgctct tccccgaatg tcatcgacgt gatgcgagac aggaagcgtg ccttctgcat
     6961 tgcatccgga ctcgtctttg cgacccgctt aaaattctca tcctgaatgg tccagtccag
     7021 atcgacgttc caggatccgg aaagcgcagg gtaatgagtg cgcgccatcc cactggagca
     7081 atgcaaaact accatgaacg ggattacaag aaacaggggt gcaatgattc tcatggcatt
     7141 cctgtatccc tcacacagga gttgtccatt catttctcag gcggtgtggg tcgcagcgga
     7201 cctggcgcgg atggcccgcc caccttgcaa ctgccgcccg ggttttatca cgcaatagaa
     7261 tgacgtatta catacatccc ccctctatta tcacgcaatt cttgttcgtt ttacatnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgcg gattacccgc ccgggtttta
     7441 tcacgcaata gaacgacgta ttacatacat cccccctcta ttatcacgca attcttgttc
     7501 gttttacatc cggaggcggt gtgggccgca agtgaaggtg gcgcggatta cccgcccggg
     7561 ttttatcacg caatagaacg acgtattaca tacatctccc gcaattatca cgcaattctt
     7621 gttcgtttta catccggaga cgatgtgggc gtctcgaaag cccgagcaat atgctatgta
     7681 gtacgtagaa ttattcccgt gcaagccacg ctcgaactca ttgctctacg cagaggaggt
     7741 agtaacctgg attcgtacag gcaatggttc caccgaaaat cgacatattc gaagtaacgt
     7801 tgccgtttcc cagatcttga ttgcccgctg tattgctcca cgcgccgcaa tcatttccag
     7861 aatttctcca tgcagtgtcc attcctgttt tgtaagagat tccagaattc acaaagccgg
     7921 cgctgagggg gaaagagaac aatcggttcg ctccggtgat ccctattacc gttgtgccgt
     7981 tggcctgtct gtattcctgg tttgcaagca gtacccagtc aatctgaccg tcccctacac
     8041 ttgcagaaac actcgcgcgg cgcactgtac ttccgacaag aaatgctttc accgtaaacg
     8101 tagcggctgt cggtcgcgcc ggatccgcag aacacatgga atctccggtc gcgatgccgc
     8161 caatgtttgc acttgcctgc gctgcctgcg tgacgtagaa ataacaaaaa cctctagatg
     8221 cacaggagaa gttcgcgttc gtaaggtatc gaaaaaacat catgatctca ttctgctgcg
     8281 tctgctgttc tgagatgctc atcgaccgcg cgcacgaact gagacacgac cccgcaatca
     8341 agagaacgca gaatttcctg agatccactg cgtttgccta ctcgcctggt cctagaattc
     8401 gaattctgac ctgacctgcc cagtctatgc ctggcctttg tgaagactcg cgcgactctg
     8461 aattgtaccc gatcaaaacg cgcgatgcgt cggcgggtgc ctctccgaag gtcggatcga
     8521 ctggaatcca ctgccctcca acatagactt cattccattg atggaagaga aaagaaggct
     8581 tcccttccat ttccatatag acgactccca gtgccaggcg cgttggaatt ccctgcgaac
     8641 gcgccagtgc cgcgaacagc acactgtgtt ctgtacaatc accttccaga tttcgcacaa
     8701 cttccgatgc gcttgcatat cctacactga agttcttctt ctcgatatga cgaaacaccc
     8761 agctgttcat gagcattact tttctggaaa tatccgtttc ctttcctacg atctttgcgg
     8821 ccagtttgat gatttccgga tcatccgagt tcacataacg atctggaaga cgattggggt
     8881 ctttcgcgtc gggagcaggt gcacgagcac ccctcggtag caagggtact ttctgtgtga
     8941 caatcagttt atctcctttc ttttcgcttt gctgatacgc cacagaggca atcattggaa
     9001 cacccttgct ggacgaaatt tcaaagcgcg attgtttgct ggctcgaggt cgatcgatcg
     9061 atccttccac tggaatgaaa aatagatcag caacatctgc gttgaactga gtcgccgccg
     9121 cttcctcgcg cgtagcgcgt gtcattttaa atccagcgga ctctaactcg agagaataaa
     9181 tggttccatc ctttcctacg agactggttt ctgccggacc tccaggaagc ggatgagact
     9241 ttacaaccga gacatccact tcgccccggg gtgtcttgaa tttggatgtt cctattactt
     9301 ccacattgta atcatttacc aggtcatccg cactatccgg cgaaaacact tcgtactcgt
     9361 aggatgtgcc tggagaaaat cctttctgtt gctgcaacaa atccgcggct ctcgggccga
     9421 ttgcctcttc tggcgccgcg actctggtct cttttatttt ccctgcactt tccgtggtca
     9481 caacaaactc atcgccgacg cgcatgccat gcgtcttcgc cggaatttgt gattcattgg
     9541 aaatgatatc aaacgcaata ggacggccgc tcttgtcttc gcgcacaatg gaggtgcgcg
     9601 atataaccac tggttctttg aaacgtttga tgcgaatttg catttcaact cgcgtctcta
     9661 tttctccggt cgactcgtct ctatccataa tgcgcgtata agtgcccatc gcctggcttt
     9721 gcatggaaat caagaaatga tcctctatca gcctctccgc gctgagggat gaaacaagtg
     9781 acaggaacag gatgatgatc aggcgcatta tgttattaga cgcacaaagc ctcaggatga
     9841 tcaactcttt ccaaccaggg gccctgagag cgattttcct tgacctcgaa ggctttcggc
     9901 tcatcttccc ctccagatgg aaaaagaatc tttatacgcg ctggtccggg atacggtggc
     9961 cacgtacgca gaacgtccct gttactgggt gaagcctgat cctcgcgcag aagtcttttc
    10021 cgcagtctcg tatttcaact ggcgtgcgga tatgaagcgc ttctcggcgt atctgatcca
    10081 caaactcagc tgtgaacccg gaacaaaagt gggtctactg tgcgacaaca gatacgaatg
    10141 gaatctgatc agcatgggta tcaccacgat tggctgcata gacgtgcctc gcggctgcga
    10201 tgcaacgcat caggatatca attacattct caatcatact gaatgcccgg tgctcgtggt
    10261 cgagcatgag aagatgctca agacggtagt tggaatgctg ggagaacttc ccagtctcaa
    10321 acacatcata tccatcgaag gtccggaaaa attcaagaac ctggatgaga ttgcttcaaa
    10381 actcgggaaa acacagcttc attttctggt ggatacgttc gcagaaggag aagagattct
    10441 cgcaaagctg ggagaaggtt ttctaaaaaa acgcggagaa gaaattagac cgcactcgat
    10501 tgccactatc atctacacat ccggcactac tggatccccc aaaggtgtaa tgctggagca
    10561 cagaagcttc tgctgggaag tcaatcaaat gcaacaggtt ctgcctttag gagagcgaga
    10621 tcgcaccgtg attttccttc cgccatggca catcgcagaa agacttctgg aaaccactct
    10681 catcgcatgc ggttccagta tggcaaatgc gagcatcata tctctggctg cggatatggg
    10741 agtaatcaaa ccaacggctc tggtgagtgt gccgcgcgta tgggaacaat tgtatcgtcg
    10801 cgtgtttgac aatgttcgca aacaaccaga aaaaacgcag aacatcttca acttcgctct
    10861 gaacgcggct caaacataca cggatatcat ggacactctt ctggatcgct tcgcggaaac
    10921 agaggatgaa gaaaaaacat ccacacttac gcgaaaggca atcgccgcgg ccatgcttcc
    10981 ggtcttcgcc atactcaata ttcccgccca gatcatcctg aagaaagtga agaatatttt
    11041 tggcggacgt ttgaagttcg cagtctccgg cgccggcgca ttgccggaac acgtggctgt
    11101 attctttcgc gcagtcggaa ttccaattct cgatggctat ggaatgacag aaacaacagg
    11161 tgtggccgcg atctctgccc tgcccttccc cagacgaggc tgtgtaggga gacctatccc
    11221 tggagctcag atccagcttc gcgatgatac aggaaaagtg atcaccaggc caggggtaaa
    11281 aggagtctgc tggcacaaag gaccgcacgt gatgcgcggc tattacaagg cagacgacaa
    11341 aacagccgaa atcatgcgtg atggttggct gaactcaggc gatatcttcg tttggaccaa
    11401 cactggtgaa atcaaattcg cgggccgtgc caaagacaca attgttctgg cgggtggcga
    11461 aaacgtagaa ccggggccaa tcgaaatgaa gctgcttgag catcctttta tttcgcaggt
    11521 cgtggtggtt ggtcaggaca agaagactct gggcgctttg atcgtaccta actatgatcg
    11581 atgcaaagaa gaattcaaga acatgggatt tgaagcccca tcagattacg ctcagtggga
    11641 cactcacaaa gagattcata aattcttcca ggacatagtc aagcagcagg tttccggttc
    11701 gcacggattt aagagtttcg agaaggtcac aggattccac atcatgcgca aggaattcga
    11761 gaaagggaaa gagatgactg aaacgatgaa gattaagcga aacgtagtgt ttgatcttta
    11821 taaaaaggaa atcgactcac tctacagaga cgattgatct ctgatctctg tagagttcca
    11881 aacgatcagg tatagttgag acggccgttg aggcgggact tttcttccat cttgatcagc
    11941 ggagcgttta catcgccgtt gatcacagct gtgctgcgaa tgtcgatttt ttcagatgcg
    12001 aaaagatttc cgttgatttc gcccagaacc agaatttgag aaccgtacac atcgccatga
    12061 actgcgctgt actgtccaac cacgactgtg tggttctttg aatcaatctt ggtggcggaa
    12121 atagcgcctt ctatgatgag gtgagtgtct accgtaaggt ctccagagat tgccatggaa
    12181 tgagtgatta cggatctatg atcatgcatt gttaaactcc tgcgtttggg tgagcgaatc
    12241 tcaagaatgc cgctggctaa cccgggctca agcattttag aaaaaaaagt tgcccgactg
    12301 tccggtcaat gtgacagaat ccgcctcaag ggccgggcca gagacggggc cttctagaac
    12361 agaaacctgg aagaaatgtt aaaaatttgg gctgcgcttc tcaagaaatg cagacaaacc
    12421 ttcgaccggt tcttttgtgt tgaagagctc tgcaaacgcg ttgatctcgc gttcgatgcc
    12481 cgcgctgagc ggaagttcca ttccactgat cacaagccag cgcgccgtct tctgagcgga
    12541 aggcgcctta ccctttaaga atttttctgc aagtgcattt gcatccgcca tcagagactc
    12601 gggtgcggaa actttttgca ccaggccaac ggaagcagcc tgttccgctg tgatgatttc
    12661 gccggaacaa accatttcga gggctctggc ccggccgatc aaacgtgaaa gcctctgcgt
    12721 tcctccgaat ccaggaatga gccccagacc cacttccgga agccccatct tagctgtact
    12781 ggaggcgatt cgaatgtcgc atgcaagagc cagttcgagg ccaccgccca gtgcgaaacc
    12841 gttgatggca gcaatcgaga tcaaatccga agcggccacc gcattcatgg tatcatgacc
    12901 attgcgagcg aattcctccg cctgctccgc gctcagtccc ttcatttctg cgatatccgc
    12961 acccgcgcaa aatgcggcgc cgtccccagt gagaatcatg acacgctggg tagggtcctg
    13021 ggcaattttg gaaacggcct cgcgaatatc ccggagcact tcgcggttca gcgcgttttt
    13081 tgcttccggc cttttgaggg ttaggatgga gagtggaccg tttgtctgga catttacaaa
    13141 agacatgagc agttttctcg gccgcgcggt ttttccgcta ttaatttctt ctaaatgaat
    13201 tctgccgggg gtcgaaactg ttaaagggga tcccaatgag acaacgcgtg ttcttcgaag
    13261 atctgagact ggaactcgaa aatctctctt cgcacggcct gaccgccctg aagacaggca
    13321 cagaagtgga agatatgtcc tttgaggaaa tccgaatcct ccgcgccctc tctcgtgata
    13381 tttgccccct ctttgtaaag atcggtggtg ccgaggctcg caatgatatt cgcgaactgg
    13441 cggaaatcga ggtcgatgcg atcatcgctc ccatgatcga gtccgtttac gcactcgaga
    13501 aattcattga aagtatgcgt gacgttctga gcccaaaagc ctacagacga attgaaaagg
    13561 gtttcaacct ggaaacagca acggcgttca atcgcatgac tgaattgctt acctcagagg
    13621 cggcgcagga actggtccag gttacagcag ccagaacaga tctttccgga tcgctgcgcg
    13681 tttcacctga acatgaaatc atttttgaat tgtgcactca tattgtagcg cgctgcaggc
    13741 agtatggtct tcgcacctca gtgggcggtg ccatcaagtc tgcttccatt gaaaaactcg
    13801 tgggtcagat tggccccgac actgtgaaca ccaggcatat ggttatggat tcggctgtgc
    13861 ttgctggaaa tccggtgtgc gttctagaaa agcatttgat gtgggaagtg cacctttacc
    13921 agtacctcgc cagttttcct tcacacaaac aggctaccca tagaaagcgt gcagaaactc
    13981 ttttgcagcg catgacaccg gacctggcgc ttgctccagt tgcagcagtt taatgcttga
    14041 agcgcagccc gtaaatcgca cctggcgtga ctggattcga ttgattcccg ccagaggggc
    14101 agactacttc ctgctcttct atctggcact ggatctggca ctcatcttgt ttcacgattc
    14161 ataccgcgac ctccttccca aaggcagtgt cacttacgtt cgctactttg acttttccct
    14221 catagcaatc tggtccatcg acttcatcct tcgccttcgc agacaggaag atcgatgggg
    14281 atatgtgcgc gcacactggt atgaagcgat tggacttttt ccgctgcatc caatgcgata
    14341 ctttctggct cttcgcgcgg ccaagatcgg cattgcgtat ttcagaatcc tcaggtctga
    14401 ccgcgatgta gggcaactcg tagtagaaga gatcacattc cgattccgcg atatatttgt
    14461 ggatacaatc gccgatgctg tgttcctcag atcactcgag agagtggatg aagtgatgag
    14521 caggctggac tatgaaacac tggctcgatc cacgtttcag aagcatcagg cgtccatgca
    14581 aaaagcagtt cacgactcca tcaattcgaa gtcaatcatc ggagacctgg ctcgtattcc
    14641 tctgatgggt acagtcgtaa acaaaatcgg agcagacgtt agctccattg tcatcgaggt
    14701 actggaaacg gaagtcactg gacgaatcat gaaggatatc acttctagca ttctaactga
    14761 gatgaatgag agggtgaagc tgctcgatct agaacgactc accggcagaa gactgaccac
    14821 aggtcgcaca aaaaagtaat cagacttccg gtaaacgcgc tcgctttctc tcg
//
LOCUS       DAORFF010000016        14376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1786_length_14376_cov_4.134348, whole genome shotgun sequence.
ACCESSION   DAORFF010000016 DAORFF010000000
VERSION     DAORFF010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14376
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1786_length_14376_cov_4.134348"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PKK76_01420"
     CDS             complement(<1..596)
                     /locus_tag="PKK76_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNL67146.1"
                     /translation="MSQISTQKRLKKLEVKIAEDQLEIEKALRLRYEVFNQEMGEGLP
                     QSASTGKDRDEYDLFCDHLIVVDHANENHVVGTYRLLTKTRALKNIGFYSAQEFDLSN
                     IYKLDFEIAEVGRSCVHPDYRDGSVITLLWAGIANYIQTHNIRVLMGCGSLHSTDAEE
                     VNMVYAFLRDEGHLVEEHLRVRPLPTSVHPGFREIELAG"
     gene            complement(724..1284)
                     /locus_tag="PKK76_01425"
     CDS             complement(724..1284)
                     /locus_tag="PKK76_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNL67147.1"
                     /translation="MRLTIPLILLCAVCSRSGFKFADTFHPAFPAPNIALQGSKGPYD
                     FSKDRAENRQVVTLVFFGFTHCPDICPTTMHRLSQAMGALNPKEQARVRLLFITIDPQ
                     RDSPKIAGDYAAGFFPGFIGLSGSPGDIDKVKTAFSATSDSQGGEITHSSGIYWIDSE
                     GNVRKKISQNFEIEDMAHDLKMVSSK"
     gene            1364..2221
                     /locus_tag="PKK76_01430"
     CDS             1364..2221
                     /locus_tag="PKK76_01430"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNL67148.1"
                     /translation="MVISIIGAGIMGKGIASNAARNGHHVRIYTRSRAKIEDILGPGI
                     EYFGKPEEAVTGAELTVVCLTEDHVVRAIVLSESFLSAISGSVMDVGTTSPELTDELF
                     RAFDKRGIRFFDAPMTGSKQAARDGKILFMMGGSDDDYAAGKPVFDSAGRAVVRCGKI
                     GDGQRAKIALNMVQAGTLQILIEGYHLSSLLGIEKTTFKEILDQSAARSGISDFKTPF
                     FLDRNYETNFSLKNMNKDLNHALSLAMSVRAELPSAFALKAVFDRAMNLGNAEKDFCS
                     IADATLNAD"
     gene            complement(2266..2853)
                     /locus_tag="PKK76_01435"
     CDS             complement(2266..2853)
                     /locus_tag="PKK76_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67149.1"
                     /translation="MKSALILFSTTFLAVTGVSADEWNISTYQGLYSQTNLGQILLQG
                     KTHYQPGYISAATVGKPTDYGIFGLKLETEGQVVRHTGRDKHMEFNGLVMARTEELFN
                     LPITFAMAEGLSLATRNPDLENPRKSIGNPGTESEYSRNLLNYLAFEVEVGVPLDAYK
                     PRAFVRVHHRSGIYGILCPPTCGSNYLGYGMRFSY"
     gene            complement(2878..3786)
                     /locus_tag="PKK76_01440"
     CDS             complement(2878..3786)
                     /locus_tag="PKK76_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNL67150.1"
                     /translation="MTVDSSRIAGAVLVFLSAVAFSAKAVIIKLAFLGPVDPITLLTL
                     RMIFSFPFFLILAVISSKKSRSPGISRGDWFLILFCGVVGYYLASLFDFLGLQYISAG
                     LERLILFIYPTFVVLISVFAFGKRVTKQIAIALVLTYAGIAFVFWHDFGTFGENAWIG
                     GGLILLSAITYAFYLAIGGRLIGRLGSMRFTTLALLVSVGAVVIHFLLTRPLHLLSDV
                     DQKTYGYAFLMAIVCTVLPTLLLASGIRRIGSSAAAIIGSVGPVSTIALAWWFLDEHV
                     DVTHAIGTILVLSGVLTVSLQKSHEP"
     gene            3820..4131
                     /locus_tag="PKK76_01445"
     CDS             3820..4131
                     /locus_tag="PKK76_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67151.1"
                     /translation="MKRILLSFLIVLFGCTTAEDQTIDADEANTRIGIAIGLKNAQCG
                     YGQFLTLPAIFPLREEAVTLCVSAIISTPCSTWAGNNPLSGACLALPFYRKKSRDNYG
                     L"
     gene            complement(4128..6404)
                     /locus_tag="PKK76_01450"
     CDS             complement(4128..6404)
                     /locus_tag="PKK76_01450"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL67152.1"
                     /translation="MADIPGLSGPIDYHKAITKKERSSRYIRTAFDYLVQVQGEDALN
                     ELLREVGIERDSSIFKHIYDDENWNSYELEVFLYEKLKDRFEDPYQAIWQFGIASGSG
                     RLDQKDTLFAFKLKIAPVPVILRKVSEHTERVTLVSECNAEILKTPATHIKNGMAATI
                     SFKYSRLPENFQYPSWTSVVAGYAIVYATVRYRKGLDFYLKITHWPNLPSDLPSVDGK
                     VYVFDKVTKNVSEKETGKVVGNARNGPFEIDGVVWNNGVEAIAWIEWERESLGQAIAK
                     LTIHRKRLKKEEELRVLRNEIVEELSAQHQKQLAAYEKELTEKARLIQAHLEKIHELK
                     IQQDGDYFLTSLLTSPMIANRNKSDAVQTEIHVEQKKKFEFRKWKSELGGDTCLTDQV
                     ILRGRQYSVFLNGDAMGKSLQGAGGAIVLGVVFNTYLNRSRFSSYQKQKSPELWLRDA
                     ALDLQNAYLSFQGSMYVSLVMGLIDETSGVMYFINAEHPWMVLYRDGKASFIGDELLL
                     RKLGTPGMEDQIRINTFQLKPGDSIIMGSDGRDDLEIGHDGSNRVINEDETIFLKVVE
                     ESKGNVESIVKNIKKQGSITDDLSLLKVSFFGSPANIENANAEYKASLGKAVALGRLN
                     KLDEAIQILHSLYRAHPDRVEPLRYLGRLQFKQKDYPAAAQTLTNFFEQHQDESGVQY
                     LISRASKKAGDYKAAIDFGERCMLREPEHLNNLINLADAYRLSGNTDRARQLVTRAVS
                     LSPDHAAARKLANLMDRS"
     gene            6490..6954
                     /locus_tag="PKK76_01455"
     CDS             6490..6954
                     /locus_tag="PKK76_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNL67153.1"
                     /translation="MDRHIVEEYRADGGLLLDAIQGLNPIQMNTRPVADTWTIQEIVV
                     HMFDSELIAVHRLRRLIAEQNPLLIGYDESAFARSLFYEKTDPVLAASMVKNLREMMA
                     DTLNLCPPEAFEKVGVHSETGRIQVADYLKKNVEHLRHHMKFLNEKKKMVQR"
     gene            complement(6956..7954)
                     /locus_tag="PKK76_01460"
     CDS             complement(6956..7954)
                     /locus_tag="PKK76_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNL67154.1"
                     /translation="MKQVSTVLLGLGRIAWMLEKDRLRYHPCTHAGSLKNPGMRKNPF
                     HLVGACDLIQERLYDFSSYWKKPLLLSRKPSEVLALRPDLAVIAASLPSHFELVRAAQ
                     KSGVHSIVLEKPPAANLKEATRLLTLCDQTNVWVNFERRYHPGYRRVKDIIDSKVLGE
                     MRSIRGRVLVGGSPGAGEAGPLLHDAIHWIDLLLWFAGKPARAFARMMRNTPDAGEHT
                     AFATFEFSDFNAVLESGGRRKYFEFEMEIDFAAGRIYCGNNGFRMEVSKPSRKYSRFN
                     ELRTAKPLLKKTQNPWQNLYQEVASVSSGETTTIHSSLASAVEAMKVIQMLEKNQR"
     gene            complement(7951..8235)
                     /locus_tag="PKK76_01465"
     CDS             complement(7951..8235)
                     /locus_tag="PKK76_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67155.1"
                     /translation="MTFLLEKDFRIGFFAGCALHLWYLIPRVSWLSLKCGARCSDIAT
                     ADAPISLLYLAFPDPGVIFFSTILGTIEWGVWFYLILKAVRFLVSRFLPS"
     gene            8244..10004
                     /gene="mutL"
                     /locus_tag="PKK76_01470"
     CDS             8244..10004
                     /gene="mutL"
                     /locus_tag="PKK76_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNL67156.1"
                     /translation="MAEKQIRRLPSIVIDRIAAGEVIESPASVIKELSENSLDAGATR
                     LRIETTAGGMDSLVVEDDGSGIPFHDLPAAIERHATSKIGSIEDIENILSFGFRGEAL
                     ASIASVSLLRLTSRTRNEEIGGILESRAGTISLHERKACSYGTVVQVRDLFFATPARK
                     KFIKSEKTENLKIYREIRKIALSSPNVEILYIRDGQEYLHFARRNTLRERLADVFPSD
                     ALDRWIEIHAESDGISVFGFISSPDAVRANRDNQLTFVNGRWVELKYMSYLLKKGYGD
                     LIAHGMHPSAVIHFTIQPDMIDVNVHPAKREIRFLDENRLQGLVIRAIQRALSAGPVV
                     FSEQTLQRNFREVSSNPLSVESLSPESSFLIEPARGAVLVSLSNQQAALPEFIRPPEK
                     PSVQIPSRLRQLGVVFGTYILAEAEDGLYLIDQHTAHERVNYEKKKKQLASIQGQRQP
                     LLHAAVIRLQPDEMQVIQTHEQELLRVGFVVEAMGKDACAVREVPLFMDPGEESDTLK
                     HTIQRVLDGEESIQIFDEYAAMKACKASIKKNDVIGDHVLADILKDLMQCDDPTRCPH
                     GRPTMVRISRDAMDRLFMRS"
     gene            10009..10527
                     /locus_tag="PKK76_01475"
     CDS             10009..10527
                     /locus_tag="PKK76_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HNL67157.1"
                     /translation="MKSVVIGVTGSIAAYRTCDLIRSLTKKGVSVHVCMTESAEHFVG
                     RITFEALTGQRVYTSQWESGMPHIELKRLSKLLAIVPATADIIGKFASGIADDLLSST
                     YLAHTGTVLIAPSMNPGMYASKAVQRNLQRLREDGVILVDPANGEAVCGDEGQGKLAT
                     IEQIESRILELI"
     gene            10524..11180
                     /locus_tag="PKK76_01480"
     CDS             10524..11180
                     /locus_tag="PKK76_01480"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HNL67158.1"
                     /translation="MISRKLIVTSGPTREWLDPVRYISNPSSGRMGWSIAKAGVGVFK
                     SVVYIAGPGSEAFRRVEGAHNVEVDTTENMLSAVLEALEKNCVLIMAAAPADYAPVEF
                     ASAKIKKDPARNLNVEFRPTPDILMEVGANAPDGMLRIAFAAETDHVRENALIKMQKK
                     KVDFICANQVFKDGMGFATESGSLLILDRNGAEKKLGPAPKDALGQELVSFVAEHLNS
                     "
     gene            11177..11395
                     /locus_tag="PKK76_01485"
     CDS             11177..11395
                     /locus_tag="PKK76_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67159.1"
                     /translation="MKIKIYSLHLGYREADAKIEKDVKGRHVLVVDGVGPVSALDYIK
                     QGMKLLSASEQEIAVLKQAGYPMKNELI"
     gene            complement(11392..12390)
                     /locus_tag="PKK76_01490"
     CDS             complement(11392..12390)
                     /locus_tag="PKK76_01490"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL67160.1"
                     /translation="MNAFLETSIPTLVLIFMMQFRNPFYVLLMPPVYVYFIFIILSAL
                     RVDRLLSIFTGLVAALEYGALSVYALLYAEPVGMDPALAAPGGFLARFLFLAAAGITT
                     GFVTRQIKHQLTASFEAGRERDKIADLFGQHVSPQVVDRLLSQNTEWLSESRHVCMMF
                     LDIRDFTRFSQNREPEQVVNYLNSLFDFMIEMINEHQGYINKFLGDGFMAVFGAPISS
                     GADVQNAVAAALKIVERVDSDSASGIIAPTRVGVGIHAGIAVTGHVGTTRKKEYTIIG
                     DVVNLASRIEQLNKEFGSKILVSEDVWKELKDHKGEELGTVTVKGRDEAVRIFKLA"
     gene            complement(12658..13917)
                     /gene="serS"
                     /locus_tag="PKK76_01495"
     CDS             complement(12658..13917)
                     /gene="serS"
                     /locus_tag="PKK76_01495"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNL67161.1"
                     /translation="MLDLKRIQDNPEELESMLAKRRFDSASLTGLKDLIKTVKTLKAD
                     ADKIRAERNSASKEIGNLIGQGKKDQAEKRKAEVKDLGDKIADLEKKCEEQEESLSDI
                     VLGLPNFIEPEVPIGDETANKTVREIGHKPQFDFKAQTHFDIGEKLGIFDFERGVKLA
                     GSRFYTYRGLGAKLERALMNFMLDLHTGEHGYQETWVPILVNDACMTTTGQYPKFKGE
                     YYRLDRDELSLIPTSEVPLINLYRDDILDEKDLPVAMTAHTSCFRREAGAAGKDTRGL
                     IRVHQFQKVELIQIVHPDQSREIHEKMVGHAEEVLKRLGLHYRVVLLSSVDMGATAAK
                     TYDLEVWMPGLDRYMEISSISNCMDFQARRGMIRFKDAAGKNKPKLAHTLNGSGVAIG
                     RALAAVIENYQKKDGTIEIPTVLQKYL"
     gene            complement(13930..14373)
                     /locus_tag="PKK76_01500"
     CDS             complement(13930..14373)
                     /locus_tag="PKK76_01500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNL67162.1"
                     /translation="MQLPIIVHLRDSQKFEPGKIQSVNDAAAMVRETKARGVLHCFTY
                     GYEEAMRFVDMGWFISFSGILTYKNAQVIQDAAARLPLECILVETDAPFLTPNPVRGK
                     PNQPAYTVHTAKFLADLRSKANGEDPDQVLAQIHQNSERFFALKK"
BASE COUNT         3565 a         3371 c         3659 g         3781 t
ORIGIN      
        1 ccggcaagtt caatttctct aaagccagga tgcacgctgg tggggagagg gcgaacgcgc
       61 agatgctcct ctacaagatg accttcatcg cggagaaacg catacaccat gttgacttct
      121 tccgcgtccg tagaatggag cgagccgcaa cccatcaaaa cacgaatgtt gtgggtctgg
      181 atgtagtttg caattcctgc ccacaggagt gtgatgacgc ttccgtctct gtaatctgga
      241 tgcacgcagc ttcttccaac ttctgcaatt tcaaaatcca atttgtatat gttggaaaga
      301 tcaaattcct gtgctgaata aaatccaatg ttcttgagtg cacgagtttt tgtaagtagt
      361 ctgtaagttc caacaacgtg gttttcattt gcatgatcca cgacgatcag atgatcgcag
      421 aatagatcgt attcatctct gtctttgcct gtggatgcag actgcggtag accttctccc
      481 atttcctgat taaacacttc ataccgcagt cgaagtgctt tttcgatttc cagctgatct
      541 tctgcgatct tgacttccaa tttcttaagc cgtttttgag tgctgatttg tgacatgttc
      601 tctcctgctt ctggagagag actaacgtta gagcatgacg aacgtgtgtc aaactcttgc
      661 cgggatgcaa ttcagtgaat gcatcctgga tcgggcggag gctcgatcca gtgcggataa
      721 ttcttatttg gaagaaacca ttttaaggtc gtgtgccatg tcttcgattt cgaagttttg
      781 tgaaatcttt ttgcgcacat tgccttctga gtcgatccag tagattcctg aagaatgtgt
      841 gatttcccct ccctgtgaat cgcttgtggc gctaaacgct gtcttcactt tgtctatgtc
      901 gcctggactg ccggacaatc caatgaagcc cggaaagaat ccggcagcgt agtcccccgc
      961 aattttggga ctatcgcgct gcgggtcaat ggtgatgaac aaaagtctta cgcgggcctg
     1021 ttcttttgga ttcagggcac ccatggcctg cgacagcctg tgcattgtgg tggggcagat
     1081 atccgggcaa tgagtgaatc caaagaaaac cagggtcacg acctggcggt tttctgctct
     1141 gtctttggag aagtcatacg gaccttttga tccctgcaaa gcaatgtttg gcgccggaaa
     1201 cgcagggtga aaggtatctg caaatttgaa tccggatcgt gagcaaaccg cacacagaag
     1261 aattagcggg atggtaaggc gcatgtgcaa cgatttggtg tgtcgtcgtc cccggcaatg
     1321 aaattgcgtt cgacgaaagt ccgcatccat cgattaggga actatggtta tttctatcat
     1381 tggcgctgga attatgggca aagggatcgc atccaatgcg gccaggaacg ggcatcatgt
     1441 tcgcatctat accaggagtc gtgcgaagat cgaagacatt ctggggccgg gaattgaata
     1501 ttttgggaag ccagaagaag cagtcaccgg tgcggaattg acagtcgttt gtctcacgga
     1561 agatcacgtg gtgcgagcga tcgtactttc ggaatccttc ctgtctgcga tttcaggttc
     1621 cgtgatggat gtaggaacca cttcgccaga actcacggat gaattgttcc gtgctttcga
     1681 caaaagagga attcgctttt tcgatgctcc catgacaggg agcaagcagg cagcgcgcga
     1741 tggaaagatt ttgttcatga tgggcggaag cgatgacgat tacgcagcgg gtaaaccggt
     1801 ctttgactct gcaggcagag cagtggttag atgcgggaag attggagacg gccagcgagc
     1861 gaagatcgcg ctcaacatgg ttcaggctgg gactcttcag atattgatcg agggatacca
     1921 cctttcgtct ctacttggaa tcgagaagac aaccttcaaa gaaattctgg atcagtctgc
     1981 ggctcgttca ggtatttcag atttcaaaac acctttcttt ctggatagga actatgaaac
     2041 gaatttttct ttgaagaata tgaacaagga tttgaatcac gctctgtctc ttgcgatgag
     2101 tgtgcgcgct gaactccctt ctgccttcgc actgaaagcc gtctttgatc gggccatgaa
     2161 cctgggcaat gcagagaagg atttctgctc cattgcggat gccactctca atgccgacta
     2221 acggtgcaaa gcgagtttat agaaggttga gaaggagagc gatcttcaat aggaaaacct
     2281 cattccgtat cccaggtagt tacttccgca cgtaggcggg cacaggattc cgtagattcc
     2341 agacctgtgg tgcactcgaa caaatgcgcg aggtttgtat gcgtcgagcg gcacccccac
     2401 ctctacttca aaggcaagat agttcaggag gtttcgtgaa tactctgatt ctgttccagg
     2461 gtttccaatg gatttgcgag ggttttccag atcgggattg cgagtggcca gagaaagccc
     2521 ttctgccatg gcaaatgtaa taggaaggtt gaagagttct tccgtgcgag ccatcacaag
     2581 gccgttgaat tccatgtgct tatcacgccc tgtgtgccgt acgacctggc cctctgtttc
     2641 cagtttcagt ccaaagattc cgtagtccgt tggttttcct accgtggcag cgctgatgta
     2701 accaggctgg tagtgcgtct ttccttgaag aagaatctgc ccgagattgg tctgggagta
     2761 gagtccctgg taggtagaaa tattccattc atctgcggaa actcccgtaa ccgcaaggaa
     2821 tgtcgtggag aatagaatca gtgcagattt catccgccgt tagacggaat acctgcctca
     2881 gggttcatgc gatttttgaa ggctaaccgt gagaactcct gacagtacaa gaatggttcc
     2941 aattgcatga gtgacatcaa catgctcatc caggaaccac caggcaagtg ctatggtcga
     3001 tactggccca acgcttccaa tgatggcggc agcgctcgat cctattcttc taattccaga
     3061 tgcgaggaga agtgtgggaa gaactgtgca gacgatcgcc atgaggaatg cgtaaccgta
     3121 agttttctgg tccacatctg aaagaagatg caacggccgc gtgagaagaa agtgaatcac
     3181 gaccgccccc acagagacca gaagggccag agtggtgaag cgcatagaac ccagtcgtcc
     3241 aatcaatctt ccaccgattg ctagatagaa ggcgtaggta atggcgctca gtagaatcag
     3301 gcctccgcca atccaggcat tctctccaaa tgttccaaag tcgtgccaga aaacgaaagc
     3361 gatccccgcg taagttagca caagcgcgat tgcgatttgt tttgtgacgc gtttgccaaa
     3421 tgcgaacact gaaatcaaaa caacgaacgt tggataaatg aaaagaatca gtctttcaag
     3481 acctgccgaa atatactgaa gtccaagaaa atcaaacaga cttgccaggt agtagcccac
     3541 gaccccacaa aataggataa gaaaccagtc ccctctgcta attccaggtg atcttgattt
     3601 cttgctcgat atcacggcca ggatcaagaa gaatggaaac gaaaagatca tgcgaagagt
     3661 cagtagagtg atcgggtcca caggaccgag gaatgccagc ttgatgatca ctgctttcgc
     3721 agagaaggcg acggcggaaa gaaagaccag gacggcgcct gcgattctgc tggaatccac
     3781 tgtcatgttt ccatttctgg gaaaggaacg tccctgaaaa tgaaaagaat tcttctgtca
     3841 tttctgattg ttttgtttgg ctgcacaacc gctgaagatc aaaccattga tgctgatgag
     3901 gcaaacactc gcattggaat tgccattgga ctcaagaatg cgcagtgcgg ttacggacag
     3961 tttcttactt tgccggccat ttttcctttg agagaagaag cagtcacact ctgcgtttcc
     4021 gctatcatat ccacaccgtg cagcacctgg gcggggaata atccgctttc gggcgcctgc
     4081 ctggctcttc cgttttacag gaagaagtcg cgagacaatt atggtctcta ggatcggtcc
     4141 atgagatttg caagctttct ggccgccgca tgatccggcg aaagtgaaac ggcccgcgtc
     4201 accagttgtc gcgctctatc cgtattgcca gaaagtctgt aagcgtctgc cagattgatc
     4261 aaattgttga gatgctctgg ttctcttagc atgcaacgct ctccaaaatc gatggcagct
     4321 ttataatcgc cagccttctt gcttgccctg gaaatcaaat actgcacgcc gctttcgtcc
     4381 tgatgctgct cgaagaaatt ggtaagagtc tgtgcggctg cgggataatc cttttgttta
     4441 aactgcagcc ttcccagata gcgaagtggt tctactcgat ccggatgcgc acgatacaga
     4501 gaatgtaaga tctggattgc ctcgtccagt ttgttgagcc ggcccagggc gaccgctttg
     4561 cccagactcg ccttgtattc agcgttcgca ttctctatgt tagccggtga gccaaaaaag
     4621 gaaactttga ggagcgaaag gtcgtccgtg atgcttccct gcttctttat gtttttgaca
     4681 atgctttcca cgttgccttt ggattcttct actactttga gaaagattgt ttcgtcttcg
     4741 ttgattacgc gattggatcc atcgtggccg atttccagat cgtctcttcc gtcacttccc
     4801 atgatgatgg aatcccctgg tttgagctgg aatgtgttga tgcgaatctg gtcttccatt
     4861 ccgggagttc ccagttttcg cagaagaagt tcgtctccga tgaaagacgc cttcccgtct
     4921 cgatacagaa ccatccacgg atgctctgca ttgatgaagt acattacacc agaagtctca
     4981 tcgattaagc ccatgaccag ggaaacatac atgcttccct gaaacgatag atatgcgttt
     5041 tgcagatcga gagctgcgtc tcgtagccac aattctggac ttttctgttt ctggtaactg
     5101 gaaaacctgg agcgattcaa gtatgtattg aatacgactc cgagcacgat tgcaccacct
     5161 gcaccctgca aggactttcc catcgcatct ccattcaaga agacagagta ttgtctgccg
     5221 cgaagaatta cctgatcggt aaggcaagta tccccgccca gttctgattt ccatttgcga
     5281 aattcaaatt tctttttctg ctctacatga atctctgttt gaaccgcatc actcttgttt
     5341 cgattggcga tcatggggct ggttagcaga gaagtaagaa agtagtctcc atcttgctga
     5401 attttgagtt cgtggatctt ttccagatgg gcctggatca atctggcttt ttctgtgagt
     5461 tctttttcat acgccgcgag ttgcttctgg tgctgtgcgc tcaattcttc gacaatctcg
     5521 ttacgcaaga ctcgcagttc ttcttctttc ttcagacgct tgcgatgaat cgttagtttt
     5581 gcaatggcct gacccagaga ctctctttcc cattcaatcc atgcgatggc ttccacgccg
     5641 ttgttccaga ctactccgtc gatttcaaaa ggtccgttcc tggcattacc gacgactttt
     5701 ccagtttctt tttcagaaac gttcttcgtg actttgtcaa acacataaac ctttccatct
     5761 acggacggta agtcggacgg aaggttaggc cagtgagtga tctttaagta gaaatcgaga
     5821 cctttacggt agcgcacggt tgcgtataca atggcatagc cggcgaccac tgaagtccag
     5881 gaaggatatt gaaaattttc aggaaggcgg ctgtacttaa aagatatggt ggcggccatt
     5941 ccgttcttaa tatgcgttgc aggggttttg agaatctctg cgttgcattc cgagaccaga
     6001 gtcactcttt ctgtgtgttc ggaaactttg cgaaggatca caggcaccgg agcgatcttg
     6061 agcttaaagg cgaataacgt atctttttga tccagtcttc cggatccaga tgcaatcccg
     6121 aattgccaga tagcctggta aggatcttcg aaacgatctt tcagcttttc gtagagaaac
     6181 acttccagtt cataggaatt ccagttctcg tcgtcgtaga tgtgtttgaa aatggaactg
     6241 tctctttcaa ttccaacttc acggagcaat tcattgagag catcttcgcc ctgaacctgc
     6301 accagataat cgaaggcagt tcgaatgtat ctggaagagc gttccttctt ggtgattgct
     6361 ttgtgatagt cgatcggtcc gctgagacct ggaatgtctg ccatgagcta acgctgctag
     6421 gtgatgactg ccaggtcaac ctaaattgcg aatcgcttga tctgtcaccc attcgaagag
     6481 gaatgtccta tggatagaca tattgttgag gaatatagag cagatggtgg tctgttgctg
     6541 gacgcaatcc agggtctcaa tccaattcaa atgaacacaa ggccagtggc ggatacatgg
     6601 acgattcagg agatcgtggt ccacatgttc gacagtgaac tcatcgcggt gcatcggctg
     6661 cgcagactca ttgcagagca aaatccactg ctcatcgggt atgatgaaag cgcttttgcc
     6721 aggtctctct tctacgagaa gacagatccg gttctggccg cgtcgatggt aaagaatcta
     6781 agggaaatga tggcggacac gctcaacctc tgccctcccg aggcattcga gaaggtgggt
     6841 gtgcacagcg aaaccggtcg gatccaggtg gcggactatc tcaaaaagaa tgtggaacac
     6901 ctgcgtcatc atatgaagtt cttgaatgaa aaaaagaaga tggtgcagcg ctagatcaac
     6961 gctggttctt ttcgagcatc tggatgactt tcatcgcttc cactgcggaa gccaggctgc
     7021 tgtggatggt ggtggtctct ccgctggaaa cggaagccac ttcctgatat agattctgcc
     7081 acgggttctg ggtcttcttg agcagcggtt ttgcagttct tagttcgttg aacctggaat
     7141 attttctaga tggttttgag acttccattc gaaagccatt gttgccgcag taaattcttc
     7201 ctgctgcgaa atcgatttcc atctcgaatt cgaagtactt tcgtcttccg cctgattcaa
     7261 gaactgcgtt aaaatcagaa aactcaaaag tggcaaacgc agtgtgttct cctgcatccg
     7321 gagtgtttcg catcatgcga gcgaacgctc gcgcaggctt tccggcaaac cacaggagca
     7381 aatcgatcca atgaatggcg tcatgcaata gcggccccgc ttctcccgct ccgggagagc
     7441 caccaacaag aactctgcct cgaatgcttc gcatttcccc caggactttt gaatcgatga
     7501 tgtcttttac tcgtctgtac cctggatggt atctcctttc aaagttcacc cacacatttg
     7561 tttgatcgca taacgttaaa agacgagtcg cctcttttag atttgcggca ggaggttttt
     7621 cgagtacgat ggagtgcacc cctgattttt gagcagcgcg caccaattca aagtgagatg
     7681 gaaggcttgc tgcaatcact gcaagatctg gccgcaacgc cagcacctcc gaaggctttc
     7741 gggataggag caaaggcttt ttccagtacg aggaaaaatc gtataatcgc tcctggataa
     7801 ggtcgcaggc acccacgaga tggaacggat tttttcgcat tccaggattt ttcagggatc
     7861 ctgcatgagt gcaggggtga tatcgtaggc ggtctttttc cagcatccag gcaattctgc
     7921 ccagacccag aagcactgtg gagacctgct tcatgacgga aggaatctgg atacgaggaa
     7981 acgaactgct ttaagaatca gatagaacca cacgccccat tctatggttc caagaatggt
     8041 ggagaagaag atcactccgg gatcaggaaa tgcaagatat agaagactga tgggagcatc
     8101 tgctgtcgca atgtcggaac agcgcgctcc gcatttcagg gaaagccagc ttacgcgcgg
     8161 aatgagatac cagaggtgga gtgcgcaccc ggcgaaaaag cctatacgaa aatccttttc
     8221 gaggaggaaa gtcatagtgg actatggcag aaaagcagat acgcaggctc ccgtctatag
     8281 taatcgatcg cattgcagca ggcgaggtga tcgagagtcc tgcctccgtg atcaaagaac
     8341 tctccgagaa ctctctggat gcgggtgcca ccaggcttag aatcgaaacg acggctggcg
     8401 ggatggatag cctggtcgta gaggatgatg gatccggtat tccctttcat gatttgcccg
     8461 ccgccatcga gaggcatgca acgtccaaaa ttggatctat cgaagacata gaaaatatat
     8521 tgagctttgg gtttagagga gaggctctcg cgtcgattgc ctctgtcagt ctactccggc
     8581 tcacaagtag aaccagaaat gaagaaatag gagggattct tgagagccgg gcgggaacta
     8641 tttccctgca cgagaggaaa gcctgttcct atggcactgt ggttcaggtg cgtgatctat
     8701 tctttgcaac accggcacga aagaaattta tcaaatcgga gaaaacggaa aacctcaaaa
     8761 tctatcgtga gatccgaaaa atcgctcttt cgtcgcctaa cgttgaaatc ctgtacatcc
     8821 gcgacggtca ggaatatctg cacttcgcaa gaaggaatac tcttcgcgaa agattggcag
     8881 acgtatttcc ctcggatgcg ctagatcgct ggattgaaat ccatgcagaa tcagatggaa
     8941 tttctgtttt cggttttatc agttcgccag atgcggtccg cgcgaatcga gacaaccagc
     9001 tcacttttgt aaatggccga tgggtggagc tgaaatacat gtcttatcta ctaaagaagg
     9061 ggtacggcga tctcatcgcg cacgggatgc atccatctgc cgtgattcac ttcaccatac
     9121 agccagacat gattgatgtg aatgttcacc ctgcgaaaag agagattcga tttctggatg
     9181 aaaacagatt gcagggtctt gtgattcgcg ccattcagag agcactgtct gccgggcctg
     9241 tcgtgttttc tgaacagact ttgcagagaa actttcgtga ggtgtcgtcg aatcctctgt
     9301 cagttgagtc tctttcgcct gagtcttctt tcttaattga gccagcgcgc ggcgcagttc
     9361 tcgtatcgct atcgaaccaa caagcggccc ttcctgaatt catacgacct cccgaaaagc
     9421 cttcggttca aataccttct cgactgaggc aactgggagt tgtgttcgga acctacattc
     9481 tagcagaagc agaagatggg ctttacttga tcgaccagca cactgcgcat gagagagtga
     9541 actacgagaa gaaaaagaag cagcttgcgt ccattcaggg ccagaggcag cccctacttc
     9601 atgcggcagt gatccgactt cagccggacg aaatgcaagt gatccagacg cacgaacaag
     9661 agcttctaag agtcggtttc gtcgtggagg ccatgggaaa ggatgcatgc gctgtgcggg
     9721 aagtgcccct tttcatggac ccgggagaag aaagtgatac gctcaagcat accatccaga
     9781 gagtactcga cggagaagaa tccattcaga tattcgacga atacgcggcg atgaaggcgt
     9841 gtaaggcttc aattaagaag aatgacgtca taggcgatca tgtcctggcc gatattctaa
     9901 aggatttgat gcaatgcgat gatccgactc gctgccctca cggaaggcca accatggtgc
     9961 gaatttcgcg cgatgcaatg gaccgattat ttatgagaag ttagatttat gaaatcagtc
    10021 gtcattggag ttacaggaag cattgccgcc tatcgtacgt gcgatttgat tcgctcgctc
    10081 acaaagaagg gtgtatctgt tcacgtgtgc atgacggaaa gcgcagaaca tttcgtagga
    10141 agaattactt ttgaagcgtt gaccgggcaa cgagtgtata cgagccaatg ggaaagcggc
    10201 atgccgcaca ttgaactcaa gcgtctttct aaacttctgg caattgttcc ggcaaccgcg
    10261 gacatcattg gaaaattcgc ttcgggtatt gcagacgatt tgctcagttc aacctatctc
    10321 gcgcacaccg gaaccgtgct gatcgcgccc tctatgaatc ctggaatgta tgcgagcaaa
    10381 gcagtgcaaa ggaatctgca gaggttacgt gaagatggcg taatactggt agatcctgcc
    10441 aacggagaag cagtgtgtgg agacgaaggg cagggaaaac ttgcaaccat tgaacaaatt
    10501 gaatcaagaa ttctggagtt gatttgattt cacgtaagct cattgtaact tctggcccaa
    10561 cccgggagtg gctcgatcca gttcgatata tttccaatcc ttccagtggt cgtatgggct
    10621 ggtcgattgc gaaagcgggc gtcggagttt ttaagtccgt ggtttatatt gccggccccg
    10681 gaagcgaagc ctttcggcgt gtagaaggcg cgcacaatgt ggaagtggat acaacggaga
    10741 atatgctgtc ggcggttctc gaagctctgg aaaagaattg cgttctcatc atggcggcgg
    10801 ctcccgcaga ttatgctccc gtcgaattcg cttctgcgaa aatcaagaag gatccagcgc
    10861 gaaatctgaa cgtagagttt cgtcctactc ccgatattct catggaagta ggcgccaatg
    10921 caccagacgg aatgcttcga atcgcctttg ctgcagagac agatcatgta cgcgaaaacg
    10981 ctttgatcaa aatgcaaaaa aagaaagtcg atttcatctg tgccaatcaa gtattcaaag
    11041 atggcatggg tttcgcgaca gagtccggct ctcttttgat tctggataga aacggtgctg
    11101 aaaaaaaact tggacctgcc ccgaaagatg ctctgggtca ggaactcgtt tcgtttgtag
    11161 cggagcatct gaattcatga aaatcaagat ttattcattg catcttggat atcgcgaagc
    11221 ggatgcgaag attgaaaaag atgttaaggg gcgtcacgtt ctggttgtgg atggggtggg
    11281 ccccgtgagt gctctggact atatcaaaca gggaatgaag ttgctttcag cttccgagca
    11341 ggagattgct gttctcaagc aggccggtta tcccatgaag aacgaactga tctaggccag
    11401 tttaaaaatg cgaactgctt catcgcggcc tttcacagtt acagtaccca gctcttctcc
    11461 cttatgatcc tttagttctt tccacacgtc ttcggacacc aggatcttac ttccaaactc
    11521 tttgttcaat tgctctatac ggctggccag gttcaccaca tctccaatga tcgtgtattc
    11581 tttcttgcgc gtggttccta catgacccgt aactgctatg cctgcatgaa ttccaacgcc
    11641 cactcgagtg ggcgctatga ttccggaggc tgagtccgaa tccacgcgct ctacaatctt
    11701 tagcgcggcc gcaaccgcgt tttgaacatc tgcgccggat gaaatcggcg cgccaaagac
    11761 agccatgaag ccatcgccta gaaacttatt gatgtaaccc tgatgctcgt tgatcatttc
    11821 aatcataaag tcgaatagac tgttaagata gtttaccact tgttctggtt ctcgattttg
    11881 tgaaaacctg gtgaagtccc tgatatccag gaacatcatg cacacatgcc tggactcgct
    11941 gagccactct gtgttttggc tcagcagtcg atctaccacc tgtggtgata catgttgtcc
    12001 aaacagatct gcgattttgt cgcgctctct gcctgcctca aatgaagcgg taagctgatg
    12061 cttgatctgt ctggttacaa atccagtggt gattcctgct gccgcaagaa acaaaaagcg
    12121 tgccagaaat cctcctgggg ctgccagggc cggatccatt cctacaggtt cagcgtataa
    12181 caatgcataa acggaaaggg ctccgtattc cagggctgcg acgagacctg taaaaataga
    12241 tagaagtctg tctactctga gtgcggataa aatgatgaaa ataaaataca catatacggg
    12301 tggcatcaag agaacataga acggattgcg aaattgcatc atgaagatga gaacgagagt
    12361 gggaatgctt gtttccagga acgcattcat gtatctccca atcgtgggat acggtcggga
    12421 agttttcaag aaatgggcag ccagagtacg cactccgagt gtacgtggct acgatggaga
    12481 agaagatggg aggaagccac cactgtagag ttccctgaaa cacttggttg aattcagcgg
    12541 gaaaagtgag cgagaagacc gtaaagaaga gagcgcctga caggaagaag acggcgagaa
    12601 ttgtggtgcg cagcatttcg ctgcgcagag tttctatttg aaaagggctg agggttctca
    12661 caggtacttc tgaagtactg tgggaatctc aatcgtgcca tcctttttct gataattttc
    12721 gatcacggca gcgagcgctc tgccaatggc gactccagac ccgttcaatg tgtgtgcaag
    12781 ctttggtttg ttttttccag ccgcgtcttt gaatcgaatc attcccctgc gagcctgaaa
    12841 gtccatgcag ttggagatag atgaaatttc catgtagcga tcgaggccag gcatccagac
    12901 ttcaagatcg tacgttttgg ctgcggttgc gcccatgtcc acagaggaaa gtagaaccac
    12961 acgatagtgg agtccaagtc tcttcagcac ttcttccgcg tgtcctacca ttttctcgtg
    13021 aatttctcgc gactgatcag gatgaacgat ctgaatgagt tccactttct ggaattgatg
    13081 cactctgatg agtccgcgag tgtcttttcc ggcagcaccg gcttctctgc ggaagcagga
    13141 agtgtgggct gtcatggcaa cgggcaggtc cttttcatcg agaatatcat cgcgatagag
    13201 attgatgaga ggtacttcgg atgttgggat cagagagagc tcatcgcggt ccaatcgata
    13261 gtattcacct ttgaactttg gatactgacc ggtcgtcgtc atgcacgcat cattgactag
    13321 aatgggaacc cacgtttctt gatatccgtg ttcccctgtg tgaaggtcga gcatgaagtt
    13381 catcaatgcg cgttccagtt tagcgcccag tccgcggtac gtatagaatc tggatcctgc
    13441 cagttttact ccgcgctcaa aatcaaagat tccgagtttt tctccgatgt cgaagtgcgt
    13501 ctgcgctttg aaatcaaact gaggcttatg cccgatttcg cgtactgtct tgtttgcagt
    13561 ttcgtctccg atcggtactt cgggttctat gaagttgggt agaccaagaa cgatgtcact
    13621 caggctctct tcctgttctt cgcatttctt ttccagatct gcgattttgt ctccgaggtc
    13681 tttgacttcc gcctttcttt tctccgcctg gtctttcttt ccctggccga tcagatttcc
    13741 gatttccttg ctcgcggaat ttctttccgc gcggattttg tctgcatctg ccttgagagt
    13801 ttttaccgtt ttgataagat ccttgaggcc tgtgagactt gcagaatcga atcggcgttt
    13861 tgcaagcatg gattcaagtt cttctggatt gtcctggatt cttttgagat cgagcatggt
    13921 tgtgttcctt tactttttaa gcgcaaaaaa gcgttctgag ttttgatgga tttgagcaag
    13981 cacctggtcc gggtcttctc cgtttgcttt tgagcggaga tctgccagaa attttgcagt
    14041 gtgcaccgtg tatgcgggct ggtttggttt tccacgaacc ggattcggag tgaggaaggg
    14101 tgcatctgtt tctaccagga tgcattcgag aggcagtctt gctgcggcgt cctgaatgac
    14161 ctgagcattt ttgtaggtaa gaattccgct gaatgaaatg aaccagccca tgtctacgaa
    14221 gcgcattgct tcttcgtatc catatgtgaa gcagtgcagt acacctctgg cttttgtttc
    14281 gcgcaccatt gctgctgcgt cattcacgga ttgtattttt cccggttcaa atttttggga
    14341 atcacgcagg tggacaataa tcggcagctg caattc
//
LOCUS       DAORFF010000017        14199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1820_length_14199_cov_3.864607, whole genome shotgun sequence.
ACCESSION   DAORFF010000017 DAORFF010000000
VERSION     DAORFF010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14199
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1820_length_14199_cov_3.864607"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="PKK76_01505"
     CDS             <1..1533
                     /locus_tag="PKK76_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNL67163.1"
                     /translation="RYASRVINRIRLFLVLLYTISVATTYSTTPPVQVTAYAIGISLQ
                     TSYCLLTFLLDWRKSVPSYWDRLTIVLDVLVLAMVMIVGVIFDTVKTISLLRTTVLYM
                     IYMFYIVCAGLAFSSTGFVLGVGFLCSLACSVVLFVAVQFGGVQLTLNGQLAQQPGYV
                     AWGAEVLKIIFIFVFAVILRSVFLILDGLRKDAEEQRAQMAESHDALSLQTQKMIAHA
                     ENLRASASAIRSVITDFNQRLEGQAAAVQQISATMEEFTASIEHSTESVRTQYQKIDR
                     MNQESAALEKLVSSVDGSSQSLDTQMETARTTGASVASAVDQLSDSLRGIEESFGRVS
                     EVNQIMAEIADRTNLLALNASIEAARAGELGRGFAIVAQEVGKLADTSSQNAGTIEHI
                     ISESARLIETGRHFADQANAMVKDQGLSLVNMSGGFKNLSGQIGEQSRIIKSLVDALR
                     ELKDLSSEIERISREQREGSSAAAQALASIESHLSTQVERARDLEESIAGIEAGAREL
                     EG"
     gene            complement(1541..2755)
                     /locus_tag="PKK76_01510"
     CDS             complement(1541..2755)
                     /locus_tag="PKK76_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNL67164.1"
                     /translation="MKSVQPKKNQYSGPVAILEDGKTKVIILGTAHVSPESIADVEKA
                     IKELDPHTVCVELCRPRYDAMLDPDRWRKLNLIRVIREKKMGLLFSSLILSAYQKKLG
                     SEKGIRPGQEMITAVQLAESSGRKLVLADRPVSITLTRAWRKVGFFSKYAIVSQLISS
                     IFVREKVTSEELEQLKAEDALADLFKTLPPRYSSIKDVFISERDLYLAEKIKHAAKEA
                     PGETILAVVGAGHLPGIRAALEAMQPVVLKDLEEVPPANPWTTAFTWLVFSALVGFVI
                     WRMTGQSEQVFKEALAAWILGRSLGAGLGAILSLAHPLTVLVAALVAPFTIFLPGSRV
                     WMFASITEVWLRKPHVEDFEDMTLVSESASGIFKALYKNRVLHLFWVIFLIQMGFLAG
                     NIYIVTFFAKHL"
     gene            2814..3320
                     /locus_tag="PKK76_01515"
     CDS             2814..3320
                     /locus_tag="PKK76_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNL67165.1"
                     /translation="MLDNIKGKVMHVGVAEWKTGKAPDALRTTLGSCVGIVLYSADKK
                     IGGVAHILLGDAPVGKIVHRGKYARPALESLLADLAKLDIKPGMLTARIFGGGSMFEA
                     VHSRFLQSIGPDNVRAATEAVKAHNIPVVAEDTGGTTGRTITLNLEDGSVLMRAGTRE
                     KYFYRVAV"
     gene            3323..4192
                     /locus_tag="PKK76_01520"
     CDS             3323..4192
                     /locus_tag="PKK76_01520"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNL67166.1"
                     /translation="MAAEEIKRQIDEQLADISRLPAMPSVVQKVVVLASDPDADVHKL
                     ADEISKDAGITAGVIRLSNSAYYRPQRPVRSVQEAVVTLGLRTVKNIVLVAASHSILS
                     QPLDGYRSEGKDIWDHSLLVAELAARIARLKRTKVQPDVAFTAGLLHDIGKIILSQYF
                     KKLYRMVAAEVEKNPAESFDSVERRIVGYDHAEIGGRLLSIWKFPAELAEAAALQYNP
                     EKAVASPELTSLVHVANHIALAAGVGVDSSGMAEPLKKQALEILRITEADLEALYGGL
                     PELLGEMNDLRTV"
     gene            4192..4731
                     /locus_tag="PKK76_01525"
     CDS             4192..4731
                     /locus_tag="PKK76_01525"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNL67167.1"
                     /translation="MQKSNIAFIGGRGAGKSRISRKFGKLSSRLSLSTDTLVCYEAGG
                     IPITKIVEREGWQGFRDREYALLEKLTAMQNLVIDCGGGILVEAPVSPGEPEQLSARK
                     MNLLKNCNVVYVKRDMSWLLDRVSADLNRPDLPGGYRELLERRLPWYEKAADFVLDMR
                     GIESDDAIALLYKKYGKIE"
     gene            complement(4734..5060)
                     /locus_tag="PKK76_01530"
     CDS             complement(4734..5060)
                     /locus_tag="PKK76_01530"
                     /inference="COORDINATES: protein motif:HMM:NF025471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4279 domain-containing protein"
                     /protein_id="HNL67168.1"
                     /translation="MPELITRDLGIEPDRVYDDGRDRIWQINSTLGATETTEEHFHEL
                     LLRLLPKRLTLRKLAEDHRLEFYCFLEKPQEMSLPITLSPRILLLCGFLHAHIDVEVK
                     DLDEEA"
     gene            5252..5986
                     /locus_tag="PKK76_01535"
     CDS             5252..5986
                     /locus_tag="PKK76_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNL67169.1"
                     /translation="MVCIPGAKFIRGSNLPSKDEDTGKMVTDEAPEEVVEVSTIFMDI
                     YEVTFSDFQKCIAAGKCRAAHPNYTGYSNPRMPMLGVNWFDSRDYCAFVGKRLPTEAE
                     WEHAARGDAGEIFPWGNEPATCARAIIQENGKKGCGPGTTVDVGSRPAYRYGLYDMAG
                     NSWEWVNDWYSPGYAKCGDDCRGKDPKGPCKGADVCPGFPEKIVKGGSWWWDAEYARS
                     SNRRPHFPANKPFHHFGFRCAASAER"
     gene            complement(6021..7880)
                     /locus_tag="PKK76_01540"
     CDS             complement(6021..7880)
                     /locus_tag="PKK76_01540"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL67170.1"
                     /translation="MDLLAEKVSHEELSNFIASLPADLRDLAVNYDTQPWRSAKEESR
                     LLDPILERYAQFGLLEQTGRDCVVTALSQTVNSLPKECGITDVVFRIPSFAAVFTTRY
                     RVYVERPEFHTFEIKTDPPAEKPDVHFLKGLIQGLMTTLAVQDAQITDGEGFTRLILP
                     DELLREGVKITYDVYAQQVLKESARVLSDNRDLQTAVEYLNMANRELEKKILVNKREL
                     DMARSIQKGFVPQRVPDWNGLQFWVKFFPFSEVSGDFYDYFYLGSTRFGMVAGDVSGH
                     GVPAALISAIAKFSFSNNRLDSPAQVLESVNMDMLNFVRGEGYLTGFYMIVDNNYGIT
                     YSVAAFPNPILFRAKTRHIEQLPGHGTLLGMFPDAGRSYMDYQTYLEPGDKLFIFSDG
                     LIEATNVQGESLGEDKLIDTILETEGMDVQRSSEHIMGMYNRFIRGTDQKDDLTLITA
                     MLSERLSEFDETVMHARRAFHSGDVTHACTLLKKAISIFPRQTAALFLLGKYLALEGK
                     YEEALEYLRQYNGLKPYNADSYRIKGYCYYRLGNLEAASEQFKRALGLRWENPSVLYN
                     QTMVLLKLNQVEEARKLFEYFEHTNPGHFLLGKIRDKLRAMNQHTEDDAPGAD"
     gene            complement(7965..8645)
                     /locus_tag="PKK76_01545"
     CDS             complement(7965..8645)
                     /locus_tag="PKK76_01545"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNL67171.1"
                     /translation="MIGITTKPVADEKYGQSFEYVDAIIRAGGIPVLVPPVGNPAEIY
                     ERLDGLILSGGGDIDPSYYGGRAHSAIYGVSKERDEFEIELARVAARGRRPCLPICRG
                     IQVLCVALGGTLIEHIPDETDESIVHRDPDMKEGKPHKVDITPNSILALALEATSCTV
                     ISWHHQAIRDPGPLRVTAKSSDGIIEAVELPGHAWMNAVQWHPEMSAGTDPTQQRLFD
                     CFVKACRS"
     gene            8697..9704
                     /locus_tag="PKK76_01550"
     CDS             8697..9704
                     /locus_tag="PKK76_01550"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNL67172.1"
                     /translation="MRLCILDFNVWSGLTYQGMFHMQTYGDLDFRHRRRHSMIEQIRT
                     LNPDVVCLHELNPVPSEAAFIAKALDMDVFWHIHLAGIRLGPIGMPWNLYEGDAIFAR
                     KSLGLKALGRRKLSGGLVTRYASFNAQDATQILAAQIQCEDRVVPIFTTHWHAGVTPG
                     PDIEDRAKRIEKEEGVEPAKIQKALEMMRGNAGIRLKEAAWTVDFVDRTLERTQAENP
                     FAILSGDFNANPGTPEIRHLLDHGFADAYSAKHDDLGPTWDYAQNDHHKTFYSDYDPD
                     LYLRLNYTRLKIPHRLDYIFYRPSPTTTLLRCEIAMKDRIAGVQASDHFGVLAEFSLD
                     G"
     gene            complement(9708..10733)
                     /locus_tag="PKK76_01555"
     CDS             complement(9708..10733)
                     /locus_tag="PKK76_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67173.1"
                     /translation="MKHILLSALGLLSMPLYADGVKYVRSEFDANQYGVRALYVMFTN
                     EGNQPAKETVTARVKAKDGSTMNFRKEETIPAHAGLTFEITGMPADASEATIEWRGKA
                     VGTVKAGALPQAVVFPATGKDCSKSGVNIPHGARIRDKGKIQRCNNGNFQLDNTTFTG
                     FCPSSFSTSQQPNGIGGDAKFRSMDISPDRARVTCSYEVNASMTYRVSSNAHSCEGDP
                     KRPRHVTCTVPILGPIASLLEGREIGGRNFAERIKVQVLEYEKIQTMGLIDSKWISGN
                     QQDSSGQVELIFESLNVPSNNKSFTVTYKSTDARLQVNLPDKDFSGCKSAGKDKFTCE
                     ANVVKEF"
     gene            complement(10940..12160)
                     /locus_tag="PKK76_01560"
     CDS             complement(10940..12160)
                     /locus_tag="PKK76_01560"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNL67174.1"
                     /translation="MEPVYVFDAVRTPRGKGKKRGVLAHIHPQNLAGDTLRALTKRNS
                     SLNAEKVEDVIMGCVSQVNEQGACVARYAVMAAEWPENVPGYTVNRFCGSGLQAINNG
                     VAAVGSGMSDLVVAGGVESMSRVKMGDDVGDLDMSIGNPFIAEHYNLVPQGISADLIA
                     TKYGFSREDVDRFAVESQQKADHAIKNGYFKNSIIPVKGPKGEDVLLDEHPRIESDYE
                     FRSGLGGVFKTYGESGADAIALKTYPDISKINHVHTLGNSSGVVDGAAVVLLGSEKAK
                     KEYGLRPRARIVSMAAIGSEPTIMLTGPVAASKLALQKAGLTAKDIDLYEINEAFAAV
                     VLFVQKELGIPKEKINVNGGAIALGHPLGGTGAILLGTLTDELERRNLRYGLATLCIG
                     GGMGIATVIERING"
     gene            12268..12891
                     /locus_tag="PKK76_01565"
     CDS             12268..12891
                     /locus_tag="PKK76_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNL67175.1"
                     /translation="MKTKSQKSQLGPREKILRTAVRLFYRQGFPNTGINQIIEESGVA
                     KASFYQWYPSKDDLARAYLDYYGDLLTSRLERLLDRSADIDEFAMRWAAILRRDVRSQ
                     GRYNGCPFLNFAAQISAGHEGLDEMPGAIIENWIHLVDRRLTRAVSTGQLPASLDTRL
                     LARRLIHAFSGAHAMWRLTHDPSCIYDLENVFRSLVRDQSLNSTPTR"
     gene            complement(12861..14159)
                     /locus_tag="PKK76_01570"
     CDS             complement(12861..14159)
                     /locus_tag="PKK76_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67176.1"
                     /translation="MARRIGLLLLLSLCPLSAEIVQLRDGRTMRGRITAQDTETISLR
                     TQDGDYLIYRDQVYRILLDDPAVLRAQEAAEAEKEARVTPQTQVRDQIREETKVPESP
                     GKRPETPGKRDEKKEEKHANFLFGLSAGGAKHDSVYKEIFVDDKTADYVQQGIALTSS
                     VSYPYEFSESKLKTYAAYMYYESGFWAVGAEGETGSHSSSYQLMESLTNSGRFYAGAG
                     SFTKTTDQIYRLRAGILAGSESWTVQPWIFAGPVFLASRSKGNQTEVLTNPTTLAAAA
                     VYSPSLQYKSSAQGLEAATEIRWLISGGMELRGKGAFGQMKGGLHYDEFRLPLQDATA
                     YAALTAYPTWVIQSAQLRTVLTQYGAAFYFPLGDMFRVFVDLGMTRVQYKLSNLGTLR
                     LPDNALAPGATANGDLYRYIFGKSFNDTSRAYRVGVEFRL"
BASE COUNT         3310 a         3481 c         3726 g         3682 t
ORIGIN      
        1 cgttatgcat ccagggtcat caaccggatc cgtttgtttc ttgtactgct ctacacgatc
       61 agtgttgcca ctacatattc taccactccg cccgtgcagg tcacagcata cgcaatcggc
      121 atatctctac agacttccta ttgtcttttg actttcctcc tcgactggcg aaagagtgtg
      181 ccttcgtact gggatcgtct gaccattgtt ctcgatgtgc tcgtgctggc catggtgatg
      241 atcgttggtg ttatctttga tacagtaaag acgatttccc tcctgcgcac tactgttctc
      301 tacatgattt atatgtttta catagtatgt gccggccttg cattttcttc gaccgggttc
      361 gtgcttggag ttgggtttct ctgctctctt gcgtgctccg ttgtgctctt tgtcgcagtg
      421 cagtttggtg gagtgcagct tacgctcaac ggtcagctgg cacagcagcc aggctatgtg
      481 gcctggggcg cggaagtttt gaaaatcatc ttcatctttg ttttcgccgt gattctgcgg
      541 agcgtgtttt tgattctgga tggcctgcgc aaagatgcag aagagcaacg agcgcagatg
      601 gcggaatcgc atgatgctct ctctcttcaa actcaaaaga tgattgctca tgcagaaaac
      661 cttcgtgcat ccgccagcgc gattcgctct gtgattacag acttcaatca acgtctcgaa
      721 ggccaggcag ctgcggttca acaaatttca gcgaccatgg aagagttcac agcttccatt
      781 gaacattcca ctgaatctgt tcgcacacag taccagaaaa tcgatcgtat gaaccaggaa
      841 agcgcggcac tggaaaaact ggtgagttct gtagatggct ccagtcaatc cctcgacaca
      901 caaatggaaa ccgcacgcac aacaggcgcg agcgttgcat cggcggtgga tcagctttct
      961 gattcgctgc gtgggatcga agaatcattt ggacgagtca gcgaagtcaa tcagatcatg
     1021 gctgaaattg cagatcgcac gaatctgctt gcactcaatg cgtcgattga agctgccagg
     1081 gccggagaac tggggcgagg ctttgcaatt gtagcgcagg aagtgggcaa gcttgcggat
     1141 acgagttcgc agaacgcagg aacgattgag cacatcatat cagagagtgc gaggctcatt
     1201 gagacaggtc gtcatttcgc agaccaggca aacgcaatgg tgaaggatca aggactcagt
     1261 ctggtcaaca tgtcaggcgg tttcaagaat ctgtccggac agattggtga gcagtcacgc
     1321 atcattaaga gtctcgtgga cgctcttcga gaactcaaag atctttcttc tgaaattgaa
     1381 cgcatttcca gggaacaaag agaaggcagc tctgcggccg ctcaggcact cgcttctatt
     1441 gaatctcatc tttccacaca ggtggaacgt gctcgcgatc tagaagaaag catcgcgggc
     1501 attgaagctg gcgcccgcga attagaaggt tgaaatcgcc ttacaggtgt ttggcgaaga
     1561 aagtaacaat atagatattc cccgccagaa aacccatctg gatcaagaag atcacccaga
     1621 acaaatggag aacccggttt ttgtaaagtg ctttgaatat gccgcttgcg gattctgata
     1681 caagagtcat gtcttcaaaa tcttctacgt gaggttttct gagccagact tctgtaatcg
     1741 aagcaaacat ccatacgcga gagccaggca gaaagattgt aaatggtgca accagtgccg
     1801 caacaagcac ggtcaaagga tgtgccagag atagaatggc tccgagccct gcccccagac
     1861 tccttcccag aatccaggcc gccagtgctt ccttgaagac ctgctctgat tggccggtca
     1921 ttctccagat tacaaagcca actagcgcag agaatacaag ccaggtgaaa gcggtggtcc
     1981 atggattggc gggtgggact tcttccagat ctttgagcac gactggctgc attgcttcta
     2041 gagctgcgcg aattcctggc aaatgcccgg ctcccactac tgccagaatg gtttctccag
     2101 gagcttcttt tgcagcgtgt ttgatttttt ctgcgaggta cagatcgcgt tcgctgatga
     2161 acacatcttt gatggaactg tatctgggcg gaagagtttt gaataaatcg gcgagtgcgt
     2221 cctctgcctt caattgttcg agctcttcag acgttacttt ttcgcgtaca aaaatggaac
     2281 tgatcaattg cgaaacaatt gcgtatttgc tgaaaaatcc aacctttctc caggcacgag
     2341 tgagagtgat gctgacgggc ctgtccgcca ggaccagttt tctaccggat gattctgcaa
     2401 gctgcaccgc cgtaatcatt tcctggcctg gtcgaattcc tttttcagag ccaagtttct
     2461 tctggtaggc agaaagaatc aaagaagaga agaggaggcc catcttcttt tcacggatca
     2521 cgcgaatgag attgagtttc ctccagcgat ccgggtcgag catggcatcg tatctggggc
     2581 gacacagttc aacacaaaca gtgtgggggt cgagctcctt gattgctttt tccacgtctg
     2641 cgatggactc gggcgaaacg tgtgctgtgc ccaggatgat gacttttgtt tttccatctt
     2701 ctagaattgc aactggaccg gaatattgat tttttttagg ttgaactgat ttcactggaa
     2761 cagattttct tccagggctg gcctgctcca gtgaaatttg agctgaggat cagttgttgg
     2821 acaatatcaa aggcaaggtg atgcatgtag gcgtcgccga atggaagaca ggcaaagcgc
     2881 cggatgctct tcgcaccact ctgggaagtt gtgtgggaat cgtactttac tcagcggaca
     2941 aaaagatagg tggggtggcg cacattctac tgggggacgc tcccgtaggt aagattgtcc
     3001 atcgcggaaa gtacgctcgc cctgctctgg aatctctcct ggcagacctc gccaaactag
     3061 atatcaaacc aggaatgctc actgctagaa tttttggcgg cggttccatg ttcgaagcgg
     3121 tccattccag gtttcttcag agcattgggc cggataatgt gagagccgca acagaagcag
     3181 tcaaggcgca caatattccg gtcgtggcag aagatacggg cggaaccaca ggcagaacca
     3241 tcacgctcaa tctggaagat ggaagcgttc tgatgcgcgc aggcactaga gagaagtatt
     3301 tctatagagt ggcggtgtaa ccatggcagc agaagaaatc aaacgccaga tcgatgaaca
     3361 gctcgccgat atttctcgtc ttcctgctat gccttccgta gttcagaagg tggtggtgct
     3421 tgcctcagat ccggatgcgg atgtgcacaa gcttgcagat gaaatttcga aggacgccgg
     3481 gattacagca ggagtgattc gtctttctaa ttctgcgtac tacagaccac aaaggccagt
     3541 gcgctccgtg caggaagcgg tggtcactct tggtcttcgc acggtgaaga acattgtact
     3601 ggtcgcggca tctcacagca tactctcaca acctctggat ggatatcgat ctgaaggaaa
     3661 ggatatctgg gatcacagtc ttctggttgc agaactcgca gcacgcattg cgcgattgaa
     3721 acgcacgaag gtacagcctg acgttgcatt taccgcggga ctccttcatg acatcggaaa
     3781 gatcatcctt tcccaatact ttaagaaact atacagaatg gtagccgcgg aagtggaaaa
     3841 gaatccggcg gaatcatttg attcagtgga aagacggatc gtcggatatg atcatgctga
     3901 gattggtggt cgtttgcttt ctatctggaa gtttccagca gaacttgcgg aagcggcggc
     3961 acttcaatac aatccagaga aggcggtggc cagtcccgag ctcacgagtc tggttcatgt
     4021 ggccaatcac atcgctctgg ctgcgggtgt tggtgtggat tccagcggca tggcagagcc
     4081 tctcaagaaa caggcgctgg aaatcttgag aatcacagaa gcggatctgg aggcgctcta
     4141 cggggggctt ccagagctac tcggtgagat gaacgatttg aggacagtgt gatgcagaag
     4201 tcgaacatcg ctttcattgg tggaagggga gcgggcaaat ccaggatctc cagaaaattc
     4261 gggaaattgt cgagcaggct ttctctttct acggatacac tggtttgcta cgaagcgggc
     4321 ggcattccca ttacgaagat tgtggagcga gaaggatggc agggtttcag agatcgcgag
     4381 tatgcactcc tcgagaagct cacggccatg caaaatctgg ttattgattg cgggggcgga
     4441 attcttgtgg aggcgcctgt ttccccggga gaaccagagc aactgtcagc cagaaaaatg
     4501 aatcttctaa agaattgcaa cgtggtatac gtgaagcgcg acatgagctg gctcctcgat
     4561 cgcgtcagcg cggatttgaa tcgtccggat cttccaggtg gttacaggga attgttagag
     4621 agaaggctgc cctggtatga gaaagcagcc gactttgtac tggatatgcg cgggattgag
     4681 tcagatgacg ccatcgcact tctttataag aagtacggca agatcgaata acgttaggcc
     4741 tcttcgtcca ggtcctttac ctccacatca atatgagcgt ggaggaatcc gcacaagagc
     4801 agaattctgg gggagagggt gatgggcaga ctcatttcct ggggtttttc aaggaagcaa
     4861 tagaattcta gtcggtgatc ttctgcgagt tttcgcagcg ttaatctttt tggcagcagc
     4921 cgcaggagta gttcgtggaa gtgctcctcc gttgtttccg tggcacccag agtggagttg
     4981 atttgccaga ttctatctct gccgtcgtca taaacacgat ctggttctat tcccagatcg
     5041 cgcgtgatga gttccggcac gatgctccca cggatcgtaa agatcgccca gcttctgact
     5101 cgcgacacgg ttcggttatt tgtttacact caaacttcac aagcatttta gcatgatgcg
     5161 cctttttaca tgtatgattt tgattctatt tggctgcact cctgccgcgt ttaaacagga
     5221 tccctgcgct gctctgcatc ctccggcggg aatggtctgc attccgggtg cgaagttcat
     5281 acgcggttcg aatctacctt ccaaagatga agacacaggc aagatggtca ccgacgaagc
     5341 gcctgaagaa gtagtggagg tgagcaccat tttcatggac atctatgaag tcactttttc
     5401 cgattttcag aaatgcattg cggccggtaa atgtcgtgcg gcccatccaa attacacagg
     5461 ctatagcaat cccaggatgc ccatgctcgg agtgaactgg tttgattcgc gcgactattg
     5521 cgcttttgta ggaaagcgtc tacccacgga agcagagtgg gaacatgcag cgcgaggaga
     5581 tgctggagaa atttttccat ggggaaacga gccggcaaca tgcgcgcgag cgatcattca
     5641 agaaaatgga aagaaaggat gcgggcctgg caccacggtg gatgtgggat caagacccgc
     5701 ttatcgctac ggactctacg acatggccgg caattcgtgg gaatgggtca atgactggta
     5761 ttcacccggt tatgcgaagt gtggagacga ttgccgtgga aaagatccaa aaggtccgtg
     5821 caagggcgca gacgtgtgtc ctggatttcc ggagaagatt gtgaagggag gatcctggtg
     5881 gtgggatgcg gaatacgcgc gtagctccaa tcggagacct cactttcctg ctaacaagcc
     5941 gtttcatcac tttggatttc gatgcgctgc ctctgcagaa cgttagacga tcttgaataa
     6001 gcttgaaaat attctagaag ttaatccgca ccgggcgcat cgtcttcggt gtgctgattc
     6061 atcgctcgca acttgtctct gattttaccc agaaggaaat ggcccgggtt ggtatgctca
     6121 aagtattcaa atagtttgcg ggcttcttcc acctgattga gcttcaacaa aaccatggtc
     6181 tgattgtaca aaacggatgg gttttcccat cgtagaccca gagcccgttt gaattgctcg
     6241 gacgcagctt ccaggttgcc cagtctgtag tagcaatatc ctttgatcct gtaagaatca
     6301 gcgttgtagg gtttaaggcc gttgtattgc ctgagatact caagagcttc ttcgtatttg
     6361 ccttcaagcg ccagatactt cccgagcaag aagagagcgg ctgtttgtct tgggaaaatg
     6421 gaaattgctt ttttgagaag agtgcaggcg tgcgtaacgt ctcctgaatg aaacgctctt
     6481 cgcgcatgca tgactgtttc atcaaactcg ctcagccttt cgctgagcat ggcagtgatg
     6541 agagtcaggt catctttctg gtctgtaccg cgtatgaacc gattgtacat tcccatgatg
     6601 tgttcagaac ttctttgcac atccatccct tctgtttcca gaattgtatc tatgagtttg
     6661 tcttctccca ggctctctcc ctgcacattc gttgcttcaa tgagaccatc tgaaaaaatg
     6721 aaaagcttat ctcccggttc caggtacgtc tggtagtcca tgtaagatct gcctgcatcc
     6781 ggaaacattc caagaagagt tccgtgaccg ggtaattgtt caatgtgtcg cgtcttcgcg
     6841 cgaaatagaa ttgggttggg gaaggcggcc acagaatacg tgatcccgta gttgttgtcc
     6901 acgatcatat aaaaaccagt gagatagcct tcccccctta cgaagtttag catatccatg
     6961 ttcacgcttt ctagtacctg tgctggcgaa tccaggcgat tgtttgaaaa agagaacttt
     7021 gcaatcgcgc tgatgagagc ggccggcact ccatgtccgg aaacatctcc agccaccatt
     7081 ccaaagcgtg tggaccccag gtagaaatag tcgtagaagt cgccggaaac ttccgaaaaa
     7141 ggaaaaaact taacccagaa ctgaagaccg ttccagtcag gaactctctg cggaacaaaa
     7201 cctttctgga tgcttcgagc catgtccagt tcgcgcttgt tgaccaggat ctttttctcc
     7261 agttcccggt tggccatgtt tagatattct actgcagtct gtaagtcacg attgtcgctg
     7321 agtactctgg ccgattcttt gagtacctgc tgtgcataca cgtcgtatgt gatcttgacc
     7381 ccttcgcgca acagctcatc gggcaaaatc aggcgagtga acccctcgcc gtctgtgatc
     7441 tgcgcatcct gcactgcaag agttgtcatc aaaccctgga tgagcccttt taagaaatgg
     7501 acgtctggtt tttctgccgg gggatccgtt ttgatttcaa aagtatgaaa ctcaggccgc
     7561 tcaacgtaga ctctgtatct ggtcgtgaac actgccgcaa aggacggaat tctgaatact
     7621 acatctgtaa tgccacattc tttaggaagc gagtttacag tctgcgagag cgcggtgacc
     7681 acacaatctc gcccggtttg ttctagaagc ccgaattgcg cataacgttc tagaattgga
     7741 tcgagcagcc gcgattcttc tttcgcactt ctccagggtt gcgtatcgta gttgaccgca
     7801 aggtcacgaa ggtctgccgg gagactggcg atgaagttgc tcaactcttc gtgactgact
     7861 ttctctgcca ggaggtccat gaagcgccgc aagcggatgg aatttacagt gccggtcttc
     7921 aacgcccaca ggatgtatgg tggtgggttt gaggaaagac ttttttagga tcgacaggct
     7981 ttcacgaagc agtcgaagag tctttgttga gtcgggtctg ttcccgcgct catttccgga
     8041 tgccattgca ctgcattcat ccatgcatgt ccgggaagct ccacggcctc aatgataccg
     8101 tcagatgact tcgcggtgac gcgaagaggc ccgggatcgc gaattgcctg gtggtgccag
     8161 gagattacgg tgcacgatgt ggcttcgagt gcaagagcga gaatagaatt cggcgttatg
     8221 tctaccttgt gtggttttcc ttctttcatg tccggatcac gatgcacaat gctctcgtca
     8281 gtttcatccg gaatatgctc tatgagagtt ccgccgaggg ctacacagag tacctggatt
     8341 ccgcggcaaa tcggcagaca gggacgtctt cctctggctg caacgcgcgc gagttcgatt
     8401 tcgaattcgt ctctttcttt gcttactccg taaatcgcag aatgagcccg gccgccgtag
     8461 taagatggat ctatgtctcc tccacctgac aggatcaggc cgtccaggcg ttcgtagatc
     8521 tccgctggat ttccgactgg cggaacaaga acaggaatgc ctccggcccg aatgattgca
     8581 tctacatatt caaaagactg tccgtacttt tcatctgcaa cgggcttcgt tgtaattccg
     8641 atcaaaggtc tccacaattt cgattgatcc tccgggccca tccaggatca tttcgcgtgc
     8701 gcctttgcat acttgatttc aacgtatggt ccggtcttac ttaccaggga atgtttcata
     8761 tgcaaacgta tggagacctg gacttcaggc acaggcgtcg ccattccatg atcgaacaaa
     8821 ttcgaacatt gaatccggac gttgtttgct tgcacgaact caatccagtt ccatcggaag
     8881 cggccttcat tgcgaaggct ctggatatgg atgtattctg gcacattcac ctggcaggca
     8941 ttcgtcttgg tccaatcggg atgccgtgga atttgtatga aggagatgcg atcttcgcac
     9001 gcaagtcact cggactgaaa gctctgggaa gacggaagct gagcggtggt ctggtaaccc
     9061 gttatgcgag cttcaacgcg caggatgcaa ctcagatcct ggcggcccag attcagtgcg
     9121 aggatcgcgt ggttccgatc tttaccactc actggcacgc aggagtcact ccgggtcctg
     9181 atatagagga tcgcgcgaag agaatagaga aagaggaagg agtggagcct gcgaaaatcc
     9241 agaaggccct ggaaatgatg cgaggtaatg caggaattcg cctcaaggaa gcggcgtgga
     9301 cggttgattt tgtggatcgc actctggagc gtacccaggc ggaaaaccca tttgcaattc
     9361 tatccggtga tttcaatgcg aatccaggaa cgccggaaat tcggcacttg ctggaccatg
     9421 gttttgcgga tgcgtattct gcgaagcatg acgatctggg tccgacgtgg gactatgctc
     9481 agaacgatca tcacaagacc ttctattcgg actatgaccc tgatctatat ctgcgtttga
     9541 attatacgag gctgaagatt cctcatcgtc tcgattacat cttctatcga ccttcgccca
     9601 caaccacgtt actgcgatgc gaaatcgcaa tgaaggacag gatcgctggt gtccaggcca
     9661 gcgatcactt tggagtactt gctgagtttt ctctggatgg atgaacttta gaattctttc
     9721 accacattgg cttcgcaggt gaacttatct ttgccggcgg atttgcatcc agaaaagtct
     9781 ttgtctggaa ggttcacctg cagccttgcg tccgtagatt tgtaagtaac ggtaaaggac
     9841 ttgttgttgg acggaacatt gaggctttca aaaatcaatt ccacctggcc gcttgaatcc
     9901 tgttgattgc ctgaaatcca tttcgaatca atgaggccca tagtctggat cttctcatac
     9961 tccagaacct gtactttgat tctctctgcg aaattccttc cgccgatttc gcggccctcc
    10021 agaagacttg cgatgggtcc aagaatagga acggtgcacg tcacgtggcg cgggcgtttg
    10081 ggatctcctt cacacgagtg tgcgttgctg gaaactctgt atgtcatgga cgcattcacc
    10141 tcatacgaac atgtaacgcg tgcacggtca ggtgaaatgt ccatcgatct gaatttagca
    10201 tcaccaccga ttccatttgg ctgttgtgat gtgctgaaag aggaagggca aaatcctgta
    10261 aaagtggtat tgtcgagctg gaaattaccg ttgttgcagc gctggatctt tcctttgtct
    10321 ctgatgcgcg caccatgagg gatgtttacg cctgacttgc tacagtcttt tcctgtagca
    10381 ggaaagacga ccgcctgagg cagcgcgccg gctttaacgg ttcccacggc cttgcctctc
    10441 cattcgatgg ttgcttcgct tgcgtctgcc ggcattccag tgatttcaaa agtcaggccc
    10501 gcatgcgccg gtatagtttc ttcttttctg aagttcattg tactgccgtc tttggctttt
    10561 actcttgcag ttactgtttc ttttgccggc tggttgcctt catttgtgaa catcacatag
    10621 agcgccctga ctccgtactg gttggcgtca aattctgagc gaacgtattt tactccatct
    10681 gcgtacaaag gcatgcttag aaggccgagt gcggacagta aaatgtgctt catatattct
    10741 cctacaatgt ggttagacga acaggtcggg atgctggtaa aaatcttttc accggcagaa
    10801 cggagatcaa ggattttctg cgcgttctga gggttgccag cgcaatatga aagggtcagt
    10861 cccatttagg aggtggttct ctggcacaaa aaaaccacgg atttctccgt ggttttgcgt
    10921 ttgtctgatg aaaatcgaat cagccgttga tgcgctcgat tacggttgca atgcccattc
    10981 caccgccgat gcagagagtg gccaggccgt aacgcaggtt tctgcgctcg agttcatctg
    11041 tcagagttcc cagtagaatc gcgccagttc ctccaagtgg atggcccagt gcgatggctc
    11101 cgccgtttac gttgattttc tctttcggaa ttccaagttc tttctgaaca aagagaacaa
    11161 ccgctgcaaa tgcttcattg atttcgtaga gatcaatgtc tttcgcagtg agtcccgctt
    11221 tctgcagagc cagtttggat gcagccaccg gaccggtcag catgatggtt ggttcagatc
    11281 caatggcagc catcgatacg atgcgagcgc gaggtctcag cccgtattct ttttttgcct
    11341 tttcgcttcc aagaagaacc acagccgctc catctacaac gcctgaagag ttgcccagag
    11401 tgtgcacgtg attgatcttt gagatgtcag gatacgtctt cagtgcgatt gcatccgcac
    11461 cgctttcgcc gtatgttttg aaaacgccac cgagtccgga acgaaactcg tagtcacttt
    11521 caatgcgagg atgttcgtcc aggagaacat cttctccctt cggtcctttt acaggaatga
    11581 tactattctt aaagtatcca ttcttgattg catgatctgc tttttgttgc gattctactg
    11641 cgaaacggtc cacatcttcg cggctgaatc catatttagt ggcaatcagg tctgctgaaa
    11701 ttccctgtgg aaccaggttg tagtgctctg cgatgaacgg gtttccaatg ctcatgtcca
    11761 gatcgcctac gtcatcgccc atctttacgc ggctcatgga ttcaactcca cctgccacca
    11821 ccagatcaga cattcccgat ccaactgcgg ccacgccgtt gttgattgcc tggagaccgg
    11881 atccacagaa acgatttaca gtgtaaccag gaacgttctc tggccattct gccgccataa
    11941 cagcgtagcg cgccacacag gctccctgct cgtttacctg cgatacgcag cccataatta
    12001 catcttctac tttctctgcg ttcagagatg agttgcgctt tgtaagtgcg cgcagtgtgt
    12061 ccccggccag gttctgcgga tgaatatgcg ccagcactcc ccgttttttc cctttgcctc
    12121 gcggtgttcg aaccgcatcg aatacataaa ctggttccat aagcgctcct ttttgcttgc
    12181 ctgcatcacc cgcgggtggt ttcttgaaca gacaagtctg tctgttgggt ccagaaaacc
    12241 ggcgccaggc tccatcgtcg agcaaaaatg aagacaaagt ctcaaaaaag ccagcttggc
    12301 ccccgagaga agatactgcg gaccgccgtg cgcctgttct acaggcaggg cttcccgaac
    12361 acggggatca accagattat agaagaatcc ggagtggcca aggccagttt ttaccagtgg
    12421 tatccctcca aagatgatct ggcgcgcgcg tatctggatt attatggaga tttacttacc
    12481 tcgaggctgg agaggctcct ggacaggagt gcggacatag atgaatttgc gatgcgctgg
    12541 gctgccattt tgaggagaga tgtgcgcagt caggggagat acaacggttg tcctttctta
    12601 aatttcgcag cacaaatcag cgcaggtcac gaagggctcg atgaaatgcc aggagccatc
    12661 attgaaaact ggatccacct ggtggacagg cgtcttacca gggcagtgtc taccggccag
    12721 ctaccggcga gtctggatac gaggcttctt gcgcgcagat tgattcatgc gttttcagga
    12781 gcgcacgcta tgtggcgatt gactcacgat ccttcgtgca tctacgatct cgaaaatgta
    12841 tttcgaagcc tcgtgagaga tcagagtctg aattctacac ccacgcgata agcgcgactt
    12901 gtgtcgttga aactttttcc aaaaatatat ctgtaaagat ccccgttggc tgtggctccg
    12961 ggtgcaagtg cattgtccgg aaggcgaaga gtacccagat tggacaattt gtactgaact
    13021 cgcgtcatgc ccagatcgac gaacactcgg aacatgtcgc ctaacgggaa gtaaaacgca
    13081 gcaccgtact gagtgagaac tgtgcgaagt tgtgcgctct ggatcaccca ggtcgggtag
    13141 gcagtgagcg ccgcataagc ggttgcatcc tgtaacggca ggcgaaactc atcatagtgg
    13201 agtccaccct tcatttgacc gaaagcaccc ttgcctctca gttccattcc tccagagatg
    13261 agccagcgaa tttctgtggc ggcttcgaga ccctgcgcgc tggacttgta ttgcagagat
    13321 ggactgtaca ccgcggcagc cgccagagta gttggattgg tgagtacttc tgtttgattt
    13381 cctttggatc tgcttgcgag aaatacaggg cccgcgaaga tccagggttg aactgtccac
    13441 gattcgcttc ctgcaaggat gcccgcgcga aggcggtaga tttgatctgt tgtctttgta
    13501 aaggatccgg cgcctgcata gaaccgaccg ctattcgtca gtgactccat gagctggtag
    13561 cttgaactgt gtgacccggt ttcgccttct gctcctactg cccagaatcc ggattcataa
    13621 tacatgtatg ccgcgtatgt tttcagtttt gattcactga actcgtaagg ataggatacg
    13681 gaactggtga gtgcgattcc ctgctggaca taatccgcag ttttatcgtc tacgaagatt
    13741 tctttatata cggagtcgtg ctttgcccct cctgcagaga gtccgaagag aaagttcgca
    13801 tgcttttctt ctttcttctc atctcgtttg cccggagttt ccgggcgctt gccgggggat
    13861 tctggcactt tcgtttcttc gcgaatttga tcgcgtacct gggtctgggg tgtgaccctt
    13921 gcttcttttt ctgcttctgc tgcttcctgg gcccgtagca ccgccggatc gtccaggaga
    13981 attctataaa cctgatcgcg ataaattaga taatccccat cctgggtgcg cagtgatatg
    14041 gtttctgtgt cctgggccgt gattcggcct cgcatggttc taccatcccg gagctgcacg
    14101 atttctgcac tcagagggca caaggacaac agtagcaata ggccaatgcg gcgggccata
    14161 gggtaccttc ggcattcatt ggacggaaat ttcgtggac
//
LOCUS       DAORFF010000018        13763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1903_length_13763_cov_3.427925, whole genome shotgun sequence.
ACCESSION   DAORFF010000018 DAORFF010000000
VERSION     DAORFF010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13763
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1903_length_13763_cov_3.427925"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="PKK76_01575"
     CDS             <1..1396
                     /locus_tag="PKK76_01575"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL67177.1"
                     /translation="LANRFMRVHNSVEELNETLEKKVEDRTRELSETLSTVQAMKTQQ
                     DGDYFLTSLLISPLNANRADSRIFGVETLISQKKKFKFKKWEAEIGGDIVTAHTIELA
                     GRRYVAFLNGDAMGKSIQGAGGALVLGTVFKSLITRTQTSTLLQSRMPERWLKECFLE
                     LQNTFVSFDGTMLISAVVGLADEATGFIYYINAEHPWVVGYYQGQARFIEDEMALRKI
                     GVYGLEGSLSVKTYQMKPGEVLILGSDGRDDLAMNVDSEGKRLINEDSDLFLRLVEKG
                     NGDLHNIFESIEKTGEIIDDVTLMRIGYLEDGPFDRVEPSAECSENLRLARQSQASDR
                     ATDAILYLNRAVVDPTCPPSAHLDLAQTHMRLKNYAEAFSSARAFTALDPQHVDGYYL
                     ASYAGKLAGEYARAADFGECVRLREPHNVRNLVNLADIYRASGDKARAGKILEEALLL
                     DAGNENAMKIKSLL"
     gene            complement(1393..2463)
                     /locus_tag="PKK76_01580"
     CDS             complement(1393..2463)
                     /locus_tag="PKK76_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HNL67178.1"
                     /translation="MKIEVRNLSKKFGQYQALSNVNIEVPSGELVSLLGPSGCGKTTL
                     LRIVAGLEFPDSGQILFSGSDASAQTVRERHVGFVFQHYALFRHMRVFDNIAFGLRVK
                     PRSIRPDKQLITKKVNELLKLVQLDHMADRFPDQLSGGQRQRVALARALAVEPNVLLL
                     DEPFGALDAGVRRELRRWLRRLHDEIQVTSLFVTHDQDEALEVSDRVIVMNQGRVEQE
                     GTPSEVYSKPASPFVLNFLGNVNLFRGRIEQGKVKIGQMDLEAPGGGQGSENRDALVY
                     VRPHELDLHRSAPADPIFQGLVNYMNLAGSMARIEVRRDDGQIVEVEMPRDRLVAEGF
                     AVGDAVFVSASRTSIFVDDYSI"
     gene            complement(2460..3299)
                     /gene="cysW"
                     /locus_tag="PKK76_01585"
     CDS             complement(2460..3299)
                     /gene="cysW"
                     /locus_tag="PKK76_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNL67179.1"
                     /translation="MRKTRSTQDSRTVQFTLIFLAVLFFALFLAVPLISVFIGAFGSG
                     LSVWADAILDPAALAAIKLTLIVAGISVPLNTLFGLVVAWTMKFEFTGKSLLLALIDL
                     PFSVSPVISGLIYVLIFGLQGWFGAWLQDHDIKIIFALPGLVLATLFVTFPFVARELV
                     SLVEAQGNEEELAALSLGASGFQIFWKVTLPNLKWALLYGVIQCNARAMGEFGAVSVV
                     SGHIRGMTNTVPLHVEILYNEYNFTASFAVASILAFLGVITLIAKTFLEKRTQQERRK
                     ASA"
     gene            complement(3296..4117)
                     /gene="cysT"
                     /locus_tag="PKK76_01590"
     CDS             complement(3296..4117)
                     /gene="cysT"
                     /locus_tag="PKK76_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNL67180.1"
                     /translation="MRKKQKVLPGFGLSLGITIFYLGIIVLLPLAALGMKAFSGGASA
                     FLDAAFDPRSIASYKISFGASLAAALVNALFGLLLAYVLVRYEFPGRAILDALVDMPF
                     ALPTAVSGIALTAVYAPTGVFGSAFARIGIKTAYSPVGIFIALVFIGLPFVVRTIQPV
                     LEEVDHEMEEAAASLGASRFQVFVKIIFPEILPALLIGTSLAFARAVGEYGSVVFIAG
                     NMPFQTEITPLLIITKLEQYDYAGAATLGAVMLTASLLILLIVSALERLTGRHEA"
     gene            4302..6008
                     /locus_tag="PKK76_01595"
     CDS             4302..6008
                     /locus_tag="PKK76_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67181.1"
                     /translation="MKDSTIGRRALLIALAAGFAVTGNLFAYETKYTDKKTDEKTENN
                     TEFKVDGVIFAGYERTDKENSGVPDANGTNARNQGFDIKRAYLNLIGNVTDGDYKGYG
                     FRVTFDGGQVLGGTAATTSASGNNIHVPELKFAYMTMPLFSAGDFGSSSLRLGMQHTP
                     TTDNQTGASMEGYWKHRYLDTLPTENVNMSSSADTGIGYIHKADYFGLHLLLANGEGF
                     RKLNAQNIQAPTTFSSIATNAAAVRTNLRSMSTTGATADSYGLDLYGALSVKPTGKSK
                     DFEVAINFPFRLQNVTGIRDEEAKTTAVDISTLSSPKLQAYVGDKRVKQDQSVGSELD
                     VSADVGGLKITLGTGGIRYKDKRASTLSYDSTGLTGLLTNIDYTRYYNIDKDAYGSSN
                     YGYLHVRYKWVGAMFRDIYGTGSSGSLSALPSKSYLQQLAEADIADGQIGNGNVTMFS
                     THGTSPTIDLGKARFRKQLMAIEFFPTPRWSIAVGMSRQTSTDPDGYKTKVSGLAQIP
                     TIGTAAGNTSVPPGSSTNAESQVNTLIVSQNNNQATLQATDIAGEVKENKQFFVRAAY
                     EF"
     gene            6353..7348
                     /locus_tag="PKK76_01600"
     CDS             6353..7348
                     /locus_tag="PKK76_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNL67182.1"
                     /translation="MKLTIPKIVLGLAALCSSPAFAQDVTLLNVSYDPTRELYKEFNA
                     AFAKYWKGKTGQTVAINQSHGGSGKQARAVIDGLEADIVTLALAYDIDEIATRSRLIA
                     PGWQKELPNNSTPYTSTIVFVVRQGNPKNIRDWDDLVKPGVSVITPNPKTSGGARWNY
                     LAARGYALKKYGSEEKAREFLKQLFANVPVLDSGARGSTTTFAKNGQGDVFISWENEA
                     YLIQNEFGAGKFQIINPSISILAEPPVTVVDKTAERRGTKKIAEEYLKYLYSDEGQRI
                     AGKHHYRPSNPAIAGQFPFPQIKLFTIAELFGGWASAQKTFFADGGEFDQIYKRK"
     gene            7352..9079
                     /locus_tag="PKK76_01605"
     CDS             7352..9079
                     /locus_tag="PKK76_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67183.1"
                     /translation="MKTQIRATLRKTTVALSLLGGSQAIMAYETSWFDKKVDEKEAIS
                     SFKADGTIFAGYERADYEKNGVLEPGGTRPATSTGFIINRAYLNLRGDVNEGPWKGAN
                     FRVTVDAGVLGNNVLGTTNSTTSTGNSAQVAELKFAYFSLPLYTTSFGDGSVRIGQQN
                     VAIVDGQAGYSNESYWKHRYIDQNAVEKSGFAGGSADRGISFIHKADYFGLHLMLSNG
                     EAFRRNNAQFVFNPTGSTGAVNVATTGVLTAAQQTQVRTSLTNLVNGNNGTTNDSYGY
                     DLYGIPSFHPTGKAKDIDISIGLPFRLQNTTGIKRQEYEFLTADISTINSPRFDYYKG
                     DARAKQDTAFGQELAAAFDFGDLKFTIGAGHALLKDKRSSAFRIDQNGLNTLVATQGS
                     KPFVDYTTFYQADQDAYGQTNWGYIHARFLSFGFIARQIYGTGSGTISALPKKSYLQQ
                     LAEVDIRDGNIGNGDVTLAASRGSSPSIDLGKARFKRETYALTWNPFARLSVALGVEK
                     LKSKDKNGETTKVNNLDNIATFSTTTTTLSSQVNSYLATQGAPLVANDFGGKEKENKQ
                     VFIRAQYEF"
     gene            9191..10102
                     /locus_tag="PKK76_01610"
     CDS             9191..10102
                     /locus_tag="PKK76_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNL67184.1"
                     /translation="MRASSIFTQLFPLWMAIAGAITFYAPWIFTWFNAPMITWGLSFI
                     MLGMGITLTFEDFRRVSRIPLQVVLGTFLHFTVMPALGWTGAKLFSLSDPFAVGLILV
                     ACCPCGTASNVINYLAKSDVPLAVTITAISTVAAVAMTPILTGMLAGSRMTVDALGLL
                     GSTLQVILLPVTLGILLKRLLPRAAEAILPFSPPAAVIIIVMIVGSVLGQNRTQIIEA
                     GPSLLGAVVFLHAGGFLIGFLLARIFLDRIASRTISVEVGMQNSGLAVVLARANFANP
                     LVAVPGAISSFVHSAIGSVLAVYWRKH"
     gene            10099..10881
                     /locus_tag="PKK76_01615"
     CDS             10099..10881
                     /locus_tag="PKK76_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL67185.1"
                     /translation="MKVSITHIDTACFLLEIGGLRILTDPVFDPPGKIYSFGFGTFSK
                     KYSAPALSPKEIGRIDLVLLSHDQHEDNLDKSGREFIKKIPLVLSTRPAAKRIPGITG
                     LDEWQSFEIQGTSVKVTATPAQHTGLRMLNPIAGKVIGFILEWPGQTNGVTYISGDTV
                     YFQGINRIAERYAKIDTAILHTGRAGFPYLTGALHYTFHAKDALKAAEVLAPRRIIPV
                     HCAGWWHFREKEEHAHEVYSKSKFADRIVQLSPGKPLVLGPE"
     gene            10931..13294
                     /locus_tag="PKK76_01620"
     CDS             10931..13294
                     /locus_tag="PKK76_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNL67186.1"
                     /translation="MRAIHFAVVTILLCCQCTWIHDRAVRSRDLTGPSLPDIVDADPR
                     AMYHPGRIRPTAVQNVQQTKTGQLVARNEFVVRLNPAQSPARFLKDLERAKIQANIIG
                     GVPTFHLVQIEIQGDVNQETTEAKLRTLPLVEDVFLHFIRTAQASPQDWHIRNYGLDS
                     VWKEFSGAGVRIAVLDTGIDDSLSTFKGRIVEPYSVLTTSARFEDGQIKKKGDVLRVV
                     DHGTRVASVAAGSGDEKGILPGVAPRAEIMPIQVMGFHVIDESIYTNDLTILEGLARA
                     MALRSHIINLSVGTDYAQILRDVHDPAARETILTAIKKESRSTQSLYNQAFARAREMN
                     IWIVAAAGNAGDSAELEPIASHPLVISVGALDSDDRRASFSNYGTAVTTYAPGVGVPT
                     YIPGGMLSASTGTSFSAPYVAGVLALLRSSSPNLTFDQARASVVRSNIIARTTILPED
                     NSTPVFDPVGLLSHGARTDQRGRFRTFATRFGDLFIATNDAPGVKLGKILDYYNRIEV
                     HSELDPESRAAGPLVATNFGVIRERVRRGAKYGEMSVVAEAPLLEDQLTFLRENISTS
                     DFIAIVCSRKMDRKCIPYLLQRLDRKQWNANTLVALWTFKEPGTHEKIEAYLALIQSN
                     QEWVDDECFAIRGLVETTSEPDAKDQKLIDQSILRYRAFEGKFGESGLVDYRADDLAF
                     ALIKAGRSEGLFLCIDTIEKIENRLETLANWGVAVSSTIPAGFGNTPEELGYRLRSLQ
                     NMLNESTTFPEKYNHQRPVEERMKVRDKFRDYAKKAEFSRARFRLAQ"
     gene            complement(13306..>13763)
                     /locus_tag="PKK76_01625"
     CDS             complement(13306..>13763)
                     /locus_tag="PKK76_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNL67187.1"
                     /translation="YSYGLVGWNSRISAFQAAYIRLSIPHVKSRIESRRAAVKQYRTH
                     LGELPSVRPPEGFEENGYLYCILYNPSERSSVEARLKEKGIGFANTYPGSMSSQAGAK
                     GYIKAAVGGDNARKLAESILNPPLFPYMTDAEVSEVVQSLRPKAAPFVG"
BASE COUNT         3769 a         3573 c         3357 g         3064 t
ORIGIN      
        1 tctcgcgaac agattcatgc gtgtccacaa ttcagtggaa gaattgaatg aaacacttga
       61 aaagaaggta gaagatagaa ctcgcgaact gagcgaaact ctgagcacgg tccaggccat
      121 gaaaacacag caggatggag actacttcct cacttctcta ctgatttcgc cgctcaacgc
      181 aaaccgtgca gatagcagaa tcttcggagt ggaaactctc atcagccaga agaagaagtt
      241 caaatttaag aaatgggaag cagagattgg cggcgatatt gtaactgcac ataccattga
      301 actcgccggc cgacgttatg tggctttcct caacggagac gccatgggga aatcgattca
      361 aggagcaggt ggagctcttg tgctcggcac tgtattcaaa tccttgatca cgcgcacaca
      421 aacgtctaca ttgcttcaaa gcagaatgcc agaacgatgg ctgaaagaat gctttctgga
      481 actacagaat acattcgtgt ccttcgatgg aaccatgctg atttcagccg tggttggtct
      541 ggcagacgaa gcaactggat tcatttatta cataaacgcg gaacacccgt gggttgtggg
      601 ctactaccag ggccaggcga ggtttattga agacgaaatg gctcttcgca aaatcggagt
      661 ctacgggctc gagggaagtc tctccgtaaa aacatatcag atgaaaccag gagaagtgct
      721 gatccttggc tcagatggtc gcgacgacct ggctatgaat gtcgactcgg aaggcaaacg
      781 tctcatcaac gaggacagcg acttattcct tcgtcttgta gaaaaaggaa atggagatct
      841 tcataatatc ttcgaatcca ttgaaaaaac aggtgaaatc atcgacgacg tgactctcat
      901 gcgaatcggt tacctggaag acggaccatt tgatagagtt gagccgtctg ccgaatgcag
      961 cgagaacctt cgccttgcgc gacaatcaca ggcatcagat cgggctacgg atgcgatcct
     1021 ctatctaaat cgcgcagtcg tagatcctac ctgtcccccc tctgcacatc tggatctggc
     1081 acaaactcat atgcgtctta agaattatgc ggaagcattc agcagcgcac gagctttcac
     1141 ggcgctggat ccgcaacacg tagatgggta ctacctggct tcctacgccg ggaaactggc
     1201 tggcgaatat gccagagctg ccgactttgg cgaatgcgtt cgcctgagag agccgcataa
     1261 cgttcgaaac ctggtaaacc tggcggacat ctatcgcgca tccggagaca aagcaagggc
     1321 cgggaaaatc ctggaagaag ctttgctcct ggacgcaggg aatgagaacg cgatgaagat
     1381 caaatcgctt ctttagatag aatagtcgtc cacaaatatc gaggtccggc ttgcgcttac
     1441 aaacacggcg tcgcccacag caaagccctc agctacaagt ctgtctcttg gcatttcgac
     1501 ttccacgatc tgcccgtcgt cgcggcggac ttcaatgcga gccatggacc cggccagatt
     1561 catgtaattt actagcccct ggaagattgg atccgcgggc gcgcttctat gcaaatcaag
     1621 ttcatgagga cgaacataga ctagagcgtc tctattctct gagccctgac cgcctcccgg
     1681 tgcttcaaga tccatctgac cgatctttac tttcccttgt tcgattcggc cgcgaaagag
     1741 gtttacatta ccgaggaaat tcaaaacaaa cgggctggcc ggtttggaat aaacttccga
     1801 aggcgttcct tcttgttcta cccgtccctg attcatcact atgacgcggt cagaaacttc
     1861 gagagcttcg tcctgatcgt gtgtgacaaa gagactcgtg acctgaatct catcgtgaag
     1921 acgacgaagc catcgtctca attcacgacg gaccccggcg tcgagtgcgc caaagggttc
     1981 atccagaagt agcacatttg gctcaacagc cagtgcacgc gccagagcaa cacgttgacg
     2041 ctgcccaccg gacaattgat ctggaaatcg atccgccatg tgatccagct ggactagctt
     2101 caaaagctca ttcactttct tcgtgatgag ttgtttgtcc ggcctgatgc tcctgggctt
     2161 tacgcgaagt ccgaatgcga tattgtcaaa aacgcgcata tggcgaaaca atgcgtaatg
     2221 ctgaaacaca aacccaacgt gccgctctcg caccgtttgt gctgaagcgt ccgaaccact
     2281 gaacagaatc tgacccgagt ctggaaactc aaggcccgcc acaattcgca agagagttgt
     2341 tttgccgcaa ccggacgggc caagtaagga tacaagttcg cccgacggga cctctatatt
     2401 gacattggag agagcctgat actgaccaaa tttttttgaa agattgcgaa cttctatctt
     2461 catgccgatg ccttccttct ttcctgctgc gttcgctttt caagaaacgt ttttgcaatc
     2521 agagttatga cgccgagaaa tgcgagaatg gatgcaaccg caaatgacgc cgtaaagtta
     2581 tattcattgt aaagaatttc aacgtgaaga ggaacggtgt tggtcatccc gcgaatgtga
     2641 ccggagacaa cggaaacagc tccgaattcg cccatggcgc gcgcattgca ctgaatgaca
     2701 ccgtacaaca atgcccactt tagatttgga agagtcactt tccaaaaaat ttgaaaccca
     2761 ctggcaccca gcgaaagagc agccagttct tcctcattgc cctgagcttc cacaagcgat
     2821 acgagctctc gggcaacaaa tggaaaagtt acaaacaaag tcgcaagaac aagtccagga
     2881 agcgcgaaaa taatcttaat atcatgatcc tggagccacg ctccaaacca gccctgcaat
     2941 ccaaaaatca aaacatagat caatccggag atgacaggcg aaaccgaaaa cggcaaatcg
     3001 atcagagcca gcaaaagtga ttttcctgtg aactcaaatt tcatagtcca ggcaaccaca
     3061 aggccgaata acgtattgag cggaacggaa atgccggcaa cgatcaacgt tagtttgatt
     3121 gctgcgagtg cggctggatc tagaattgcg tccgcccaaa cggacaatcc agaaccaaag
     3181 gccccgataa aaacggaaat caggggaacg gccagaaaga gcgcgaaaaa caacactgca
     3241 agaaagatga gcgtaaactg gactgttcgc gaatcttgag tggagcgggt tttcctcatg
     3301 cctcgtgcct ccccgtcagt cgctctagag cgcttacgat caaaaggatc aatagcgacg
     3361 cagtcagcat cacagcgccg agcgtggctg ctccggcgta atcatattgt tccagtttag
     3421 taatgatcag aagtggtgtg atttctgttt gaaaaggcat attgcccgcg ataaaaacaa
     3481 cagagccata ctctcctacg gcacgcgcga aagccagaga cgttccaatc aagagagccg
     3541 gcagaatctc aggaaaaata atctttacaa agacctggaa gcgactcgcg cccagacttg
     3601 cggctgcctc ttccatctca tggtccactt cttcgagcac cggttgaatg gtgcgaacca
     3661 caaatggaag accaatgaat accagagcaa tgaatatgcc cacgggcgag taggccgtct
     3721 tgattccaat gcgcgcgaag gcactgccga aaacacccgt cggtgcataa accgcagtca
     3781 atgcaattcc agagactgca gtggggagag caaagggcat gtcgaccaga gcatctaaaa
     3841 tcgcgcggcc aggaaactcg taacgcacca gaacgtaggc cagaagcagt ccgaacaatg
     3901 cattgacaag cgcggctgcc agagacgcgc caaagctgat tttgtaggac gcaatggacc
     3961 ttggatcaaa agcagcatcc agaaacgcgg atgcgccacc tgagaacgct ttcattccga
     4021 gggccgccag aggcaaaagg acgataattc cgaggtaaaa gatagtgatt cccagagaga
     4081 gcccaaaacc cggcaaaacc ttctgcttct tcctcatgga acaaaaaaaa tcgagtccac
     4141 cgccgggtca atctaataac gactcacttg gtaggatttc aagggaggtc cgattgaaac
     4201 cgtcaaatcc ttgcctcgtt gtcctcggat tgtaacaaag tccagacatt actggagatc
     4261 aaccggcaca gatgtcggcg ctaacaatcg gagataatag aatgaaggac agcaccattg
     4321 gacgaagggc cctgctaatc gcccttgccg caggatttgc ggtcactgga aacctttttg
     4381 cgtacgaaac taagtacact gacaaaaaga cagatgaaaa aactgagaat aacacagaat
     4441 tcaaagtaga cggtgtgata ttcgccggat acgaaaggac agacaaagaa aacagcggcg
     4501 taccggacgc aaacggaacc aatgctcgca accagggctt tgacatcaaa cgcgcatatc
     4561 tcaacctgat tgggaacgtc accgacggcg actacaaggg ctacggattc cgcgtaacgt
     4621 ttgatggcgg tcaggtgtta ggcggaacag cggccactac gtccgcttca ggaaacaaca
     4681 tccacgttcc agaactgaag tttgcctaca tgaccatgcc tctcttttca gcgggagatt
     4741 ttggatcgag ctctcttcgc ctcggcatgc agcatactcc caccaccgac aaccagactg
     4801 gcgcgtccat ggaaggatac tggaagcatc gctacctgga cactctgcct acggaaaacg
     4861 taaacatgtc ttcttcagcg gatacaggga ttggatacat tcacaaggcg gactactttg
     4921 gactccatct gctccttgca aacggagaag gctttcgcaa actgaatgct caaaatatcc
     4981 aggcccctac cacgttttca agcatcgcaa cgaacgcggc cgctgttcgc acgaacctgc
     5041 gttctatgtc cacaacaggt gcgactgcag acagctacgg cctcgatctt tacggagccc
     5101 tctctgtaaa gccaaccgga aaatcaaaag attttgaagt tgccatcaac tttccgtttc
     5161 gcctacagaa cgttacaggc attcgagatg aagaagcaaa aacaactgcc gttgacatca
     5221 gcacgctatc gagcccgaag cttcaggcat atgttggcga caaaagagtg aagcaggatc
     5281 aatctgttgg ttcagaactc gacgtatccg cagacgtggg tggtctcaag atcactcttg
     5341 gaacaggcgg catcagatac aaagacaaga gagcaagcac tctgtcctat gattccaccg
     5401 gcctcaccgg tttgctcaca aacatcgact acactcgcta ctacaacatt gacaaagatg
     5461 cttatggatc gtctaactac ggttatttgc atgtgcgtta caaatgggtc ggcgccatgt
     5521 tccgcgatat ctacggaact ggttccagtg gatctctgag tgcacttcca agcaaaagct
     5581 atctccagca gctagcggaa gcagacattg ctgacggaca aattggaaat ggaaacgtaa
     5641 ccatgttctc tacgcatgga acgagcccga ccatcgacct tggtaaggca agattccgta
     5701 aacagctgat ggcgattgaa ttctttccca ctccacgctg gtccatcgca gttggcatga
     5761 gcagacagac ttccacagat ccggatgggt acaaaacaaa ggtgagtggc ctcgcacaaa
     5821 ttccaacgat tggaacggca gcaggaaaca cttctgttcc tccgggttct tcaaccaatg
     5881 cggaaagcca ggtgaacact ctgattgtat cgcagaacaa caatcaggct acacttcagg
     5941 caacagacat cgcaggcgaa gtcaaagaaa acaaacaatt cttcgtccgc gctgcttacg
     6001 aattctaaaa ttcgcaagcg ttcctgcttc aaaacccgcc ttcttaaggc gggttttttg
     6061 ttttgtcggc agagaacacc caattccccg ccatgcccgg atctctagaa tccaacccca
     6121 acaacaacac ttctaccttt cacggcgggc ctccccttgc cttaagacga taaaaatagt
     6181 cgtatatttt aattgacgag ccggaagcct ttgctttcta tggctcaatt agcgattgaa
     6241 atagtcgtat ataaaatcca gactccgaca ttctcttcat gaaagaagga acccgaaagg
     6301 agcccgggct ttaatgacga ctgatttgat cgcatatttc aggagtatcc ctatgaaact
     6361 gaccattccg aaaatcgtcc tggggctcgc agcactctgt tcatccccgg ctttcgcgca
     6421 ggacgtgaca ctactcaatg tctcgtacga tccaacacgc gaactctata aggaattcaa
     6481 cgcagcgttt gcaaaatact ggaaaggcaa gacaggccag acagttgcga tcaatcaatc
     6541 acacggtgga tctggcaagc aggcgcgcgc agtcatcgac ggactggaag cggatatagt
     6601 cactcttgca ctggcgtatg acattgacga aatcgcaacg cgatcgcgcc tgattgcgcc
     6661 tggatggcag aaggaacttc caaacaacag cacaccttac acctcgacca ttgtctttgt
     6721 agtaagacag ggaaatccaa aaaatattcg cgactgggac gatctggtga agccaggagt
     6781 ttccgtaatc acgcctaacc caaaaacatc cggcggcgca agatggaatt acctggcagc
     6841 acgcggttac gctctgaaga aatacggaag cgaagagaag gcgcgagaat ttctcaaaca
     6901 acttttcgca aacgtgcctg tgctggattc cggagccaga ggatctacaa caacattcgc
     6961 aaagaacggc cagggagatg ttttcatttc ctgggaaaac gaagcgtatt tgatccagaa
     7021 cgaatttgga gccggtaaat tccagatcat caatccatcc atcagcattc tcgcagaacc
     7081 gccagttacg gttgtagaca agacagcaga aagacgcgga acaaaaaaga tcgctgaaga
     7141 atacttgaag tatctgtact cagacgaagg gcagcgcatc gcaggcaaac atcactatcg
     7201 cccgagcaac cctgctatcg cgggccagtt ccctttcccg caaatcaaac tcttcaccat
     7261 tgcagagctc tttggtggct gggccagtgc tcagaagact ttcttcgcag acggcggaga
     7321 atttgaccag atctacaaaa ggaaataaga catgaaaaca cagatacgag caacactacg
     7381 caaaactaca gtcgcgctct cccttctggg aggcagccag gcaatcatgg cctatgagac
     7441 aagctggttt gacaagaaag tagacgagaa agaagcaatc agttctttca aagcagacgg
     7501 tacgatcttt gccggatatg aaagagccga ctatgaaaag aatggcgttc tggaaccagg
     7561 cggtacgaga cccgcaacgt ctacagggtt tatcatcaac cgtgcatact tgaatctgcg
     7621 cggtgatgta aatgaaggtc cgtggaaagg cgcaaacttt cgcgtaaccg tggacgcagg
     7681 cgttcttgga aacaacgtcc tgggaactac gaactcgacc acttctacag gtaacagcgc
     7741 gcaggtggca gaattgaaat tcgcatactt ttcgctgccc ttgtatacca catctttcgg
     7801 agacggtagt gtccgcattg gccaacaaaa cgttgcgatt gtagacggtc aggcgggcta
     7861 ttccaacgaa agctattgga aacacagata catcgaccag aatgcagtgg aaaaatctgg
     7921 atttgctggc ggatctgcgg atagaggcat ttccttcatt cacaaagcgg attactttgg
     7981 tctgcatttg atgctttcga acggagaagc gtttcgcaga aacaatgctc agttcgtctt
     8041 caaccccact ggcagcacgg gtgccgtcaa cgttgccaca acaggtgtat tgactgcagc
     8101 gcagcaaact caggtgagaa cgagccttac aaacctggtg aatggaaata acggaaccac
     8161 gaatgactcg tacggatatg atctctatgg gattccgagc tttcatccaa caggcaaagc
     8221 gaaagatatc gacatatcga taggccttcc atttcgcctg caaaacacaa cggggatcaa
     8281 acggcaggaa tatgaattcc tgactgcaga tatttctacc atcaactccc cgcgttttga
     8341 ctattacaaa ggggatgcca gagcaaagca ggacactgca ttcggtcagg aacttgctgc
     8401 agccttcgac tttggcgatc taaaattcac gatcggcgcg ggacacgctc tcctcaaaga
     8461 caagcgatcc tctgcttttc gcatagatca aaacggcctg aacactctcg tggcaacaca
     8521 gggctcaaaa ccgttcgttg actacaccac gttctaccag gcagatcagg atgcgtacgg
     8581 tcaaaccaac tggggctaca tccacgcgcg cttcttgtcg tttggattca ttgcccgcca
     8641 aatttacggt actggttctg gaaccatatc cgcccttccg aagaaatcct acctgcagca
     8701 acttgcagaa gtggacattc gcgacggaaa tattggcaac ggcgacgtga ctctggcggc
     8761 ctctcgcgga tcctcacctt cgatcgatct aggaaaagca cgtttcaaac gcgagacgta
     8821 cgcactgacg tggaatccct tcgcacgcct ttctgttgct ctgggtgtgg agaaactcaa
     8881 atcaaaagac aaaaatggag aaaccacgaa ggtaaacaac ctggacaaca tcgcaacgtt
     8941 cagcacaacg acgaccactc tctcaagcca ggtaaacagc taccttgcaa ctcagggtgc
     9001 gcctctcgtt gcgaatgact ttggcgggaa ggaaaaagag aacaaacaag tcttcattcg
     9061 cgcgcaatac gaattctaaa gaaaccatag aatttctccg aaagcaagtt cgccgattgt
     9121 tccgcccgcc cctccccggg cgggcttttt tttcgttgct ttcattcgcg gcagtcctag
     9181 cgtcgaagac ttgagggctt cgagtatctt cacccagctt tttcctttgt ggatggcaat
     9241 cgctggcgcc atcacgtttt acgcgccctg gattttcacc tggttcaacg ctccaatgat
     9301 tacatggggc ctgtctttca tcatgctcgg aatgggaatc actcttacat ttgaggactt
     9361 tcgccgcgta tcgcgcatcc ctttgcaagt agtccttggc accttccttc atttcactgt
     9421 gatgccagcg cttggatgga caggggcaaa attgttttct ctgagtgatc catttgcagt
     9481 tggcttgatt ctggttgcct gttgtccgtg tggaacggcg tctaacgtca tcaactacct
     9541 ggcaaaatcg gatgttcctc tggctgtgac catcactgcg atctcaactg tggctgcagt
     9601 tgccatgact ccaatcctga cagggatgct tgcaggaagc agaatgactg tggatgccct
     9661 gggcctgctc ggatctacgc tccaggtgat tctactgccg gtcactctgg gtatcttact
     9721 caaaaggctc cttcctcgcg cagcagaggc gattcttcct ttctccccgc ctgcggctgt
     9781 gatcatcatt gtgatgatcg taggttcggt cctgggtcag aatcgcacgc agatcatcga
     9841 agcaggaccc tctcttcttg gtgcggttgt attccttcat gcgggtggtt tcctgatcgg
     9901 gtttctactt gcgcggatat tcctcgatcg catagcatcg cggaccattt cagtggaagt
     9961 tggaatgcaa aactcgggtc ttgcagtggt tctcgcacgc gcgaatttcg caaatcccct
    10021 ggtggctgtt ccgggtgcga tttcttcttt tgtgcattca gcaattggaa gtgtactggc
    10081 cgtttactgg aggaaacatt gaaagtctcc atcacacaca tcgacacagc atgctttctg
    10141 cttgaaatcg gcggcctgcg catactcaca gatccagtct ttgatccacc gggaaaaatc
    10201 tacagttttg gtttcggtac tttctcaaag aagtattccg cgccggcact cagcccgaag
    10261 gaaattggac gaattgatct tgttctactc tcccacgacc aacacgaaga caacctggat
    10321 aaaagcggaa gagaattcat aaaaaagata ccgctcgttc tatccaccag gcctgcggcg
    10381 aagcgaatcc ccgggatcac tggacttgat gaatggcaga gtttcgaaat acaaggaacc
    10441 agtgtaaagg ttacagcaac gccggcgcag catacaggtc ttcgcatgct aaatccaatt
    10501 gcaggcaagg tgattggatt catactagaa tggcccggcc agaccaacgg ggtgacctac
    10561 atttccggag atactgttta cttccaggga attaatcgca tagcagagcg ttatgcgaaa
    10621 atagacactg ccatcctcca cactggaagg gccggatttc catatctgac aggagcgctc
    10681 cactatactt ttcatgcgaa ggacgcgctc aaagcagctg aagtgcttgc tccacgcaga
    10741 atcatcccgg tgcattgtgc agggtggtgg catttcaggg aaaaagaaga acacgcgcat
    10801 gaagtgtact caaagagcaa gtttgcagac aggatcgttc aactttctcc agggaaaccc
    10861 ctggttctcg gtccagaata atactgagtt tttctcttac cgggaaatgg atcaggtcgt
    10921 caaatcagct atgcgtgcca tccactttgc ggttgtgacc attctcctct gctgccagtg
    10981 cacatggatc cacgacaggg ccgtgcgctc cagagatctg acaggaccat ctcttcctga
    11041 cattgtagat gcagatccgc gtgccatgta tcatccgggt cggatcagac caacagctgt
    11101 acagaacgtt caacagacta aaacaggtca gctggtcgca cgaaacgaat tcgtagtccg
    11161 gttgaatcct gcgcagtctc cagcccgctt cctcaaagac ctggagcgcg cgaagattca
    11221 agcaaacatc attggaggag ttcctacatt ccacctggtt caaattgaaa tccagggcga
    11281 cgtcaatcaa gaaacgacgg aagcgaaact ccgcacgttg ccgctggtgg aggacgtatt
    11341 tttgcacttc attcgcacag cacaggcctc accgcaagac tggcatatta gaaactacgg
    11401 actggactcc gtatggaaag agttctcagg cgcgggagtg cgcattgcag ttctggatac
    11461 aggcatagac gattcgctgt cgactttcaa aggtcgcatc gtcgaaccgt attctgtact
    11521 cacaacatcc gctcgctttg aggacggcca gatcaagaaa aagggagatg tactgcgcgt
    11581 cgtggatcat ggaacgcgcg tggcaagtgt ggcagcgggg agcggagacg aaaagggcat
    11641 tctacctggc gttgcacccc gggcagaaat tatgcccatt caagtcatgg gattccatgt
    11701 cattgacgaa agcatctaca caaacgacct caccattcta gaaggcctgg cgagagcgat
    11761 ggcgcttcgc tctcatatca tcaacctcag tgttggcact gactatgcac aaatactgcg
    11821 cgatgttcac gatccagcgg cacgcgaaac catcctgacc gcgataaaga aagaaagtcg
    11881 ttccacacaa tctctgtaca atcaggcatt cgcacgcgcg cgcgaaatga atatctggat
    11941 tgtcgcggca gccggcaacg ccggagattc tgcagagctt gaaccaattg cttcgcatcc
    12001 tcttgtgatt tcagtgggag ctctcgattc agatgatcgt cgcgcttctt tttccaatta
    12061 cggtaccgca gtcacaacat atgctcctgg cgtcggagtt cccacatata ttcctggagg
    12121 catgctgagt gcatcaacag gaacgtcctt cagcgctcca tatgtggccg gtgttctcgc
    12181 actattgcga tcgagcagcc cgaacctgac ttttgatcaa gctcgtgcga gcgtggtgcg
    12241 atccaacatt atcgcgcgga ccacaattct tccagaggac aactccaccc ctgtttttga
    12301 tcctgtcggc cttctcagtc acggagctcg cacggatcaa agaggaagat ttcgcacgtt
    12361 tgcgacgcgt ttcggagatc tgttcattgc aaccaacgac gctccaggtg tgaagctcgg
    12421 gaaaattcta gactattaca atcgtatcga ggttcattct gagctagacc cagaaagcag
    12481 ggctgcggga ccgctggtag ccaccaattt tggtgtgatc cgggagagag ttcggcgcgg
    12541 cgcgaaatat ggggaaatgt ccgtcgttgc tgaagcgccg ttgcttgaag atcagctaac
    12601 gttcctccgt gaaaatattt cgaccagcga tttcattgca atcgtatgct ctcgcaagat
    12661 ggatcgcaaa tgcattccgt atctactcca gagactggat cgaaagcaat ggaatgcaaa
    12721 tactttagtt gcgctttgga ctttcaaaga acctggcaca cacgagaaaa tcgaagcata
    12781 cctcgcactg attcaatcca atcaagaatg ggtggacgat gaatgcttcg cgatccgcgg
    12841 attggtcgaa acgacgagcg aaccggatgc caaagaccag aaactgatcg atcagtctat
    12901 cctgcgatac cgcgcattcg agggcaagtt cggcgagtcg ggccttgttg actacagagc
    12961 ggatgatctg gctttcgcac tcataaaagc tggccggtca gagggacttt ttctctgcat
    13021 agacacaatc gagaagattg aaaatcgatt ggaaacactc gcaaactggg gtgttgccgt
    13081 ttcctctacg atcccggccg gatttggaaa tacacccgag gaactgggat accgtttgcg
    13141 ctccctgcaa aacatgctga acgaaagcac tacttttcca gaaaagtaca accaccagcg
    13201 ccctgtagaa gaacgcatga aagtgcgcga taaattcagg gactatgcga agaaagcgga
    13261 gttttcacgc gcacgcttca gactcgcaca atagtgttct cccgttcaac caacaaacgg
    13321 cgcggcttta ggtcgcaggc tctgcactac ttctgaaacc tctgcatccg tcatgtacgg
    13381 aaacagtgga ggattgagaa tgctttctgc aagcttgcgt gcattgtctc caccgactgc
    13441 tgctttgata tagcctttcg cgccagcctg cgaagacata cttccgggat atgtatttgc
    13501 aaatccgatc cccttctctt tcaaccgcgc ttctacagaa gatctctccg atggattgta
    13561 aagaatgcag taaagataac cattttcttc aaatccttcc ggcggacgaa cagaaggaag
    13621 ttcccccaga tgagtgcggt attgtttcac ggccgctctt ctgctttcga tgcgagattt
    13681 cacatgcgga atggaaaggc gaatgtaagc tgcctgaaaa gcagagatcc tggaattcca
    13741 accaaccaga ccataagagt aat
//
LOCUS       DAORFF010000019        13655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_1921_length_13655_cov_3.766838, whole genome shotgun sequence.
ACCESSION   DAORFF010000019 DAORFF010000000
VERSION     DAORFF010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13655
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1921_length_13655_cov_3.766838"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..1387)
                     /locus_tag="PKK76_01630"
     CDS             complement(257..1387)
                     /locus_tag="PKK76_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNL67188.1"
                     /translation="MEEASLYISVISLAFLAFLSILLLNELRTGAGNFYLTGVVFIAA
                     LASLLRFSVRTANPLLLILPFLVFPSGFLVGPLLYLYARHTLYGSPPGRFEIYAFSAV
                     TLFVFASHTVLSSVFPEMRSMSDVQLQVGFVTVYTRSLIFTSCIYNGAFIVRALLLVS
                     RYEAEVAQHFAGSVRDQVLWLKSLFGSTLLFFVLYFTSTVAAWIAGFSLPATPAEGIV
                     IVAMAYLVLFYYIKKPQVFALRMEQTSNVRYARQNLADADRLEHMRRLEEYMVREKPY
                     LDQRISLSDISESLSIPVHHLSMVINIEKKQNFFGFINSYRIAAAKAILSSAEFAERT
                     LLDAGFMSGFQSKAAFNRVFKEHTGMTPGQYRAFAQKKITSP"
     gene            complement(1418..2041)
                     /locus_tag="PKK76_01635"
     CDS             complement(1418..2041)
                     /locus_tag="PKK76_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67189.1"
                     /translation="MSLITQCSSINSGYYLRAPTNSAITSCAIGPDESVECARISFEG
                     MNLLFWAGTPTHPTMRGPCLIPIIPEDQPILDTYDLRLHMRILDAGKLNADVNLLSDS
                     LAALSPAGVQKPRGITEYNFHQGVDSILEYYGADSSWPRAFKINSDRTYEIWLPGIIY
                     AQTDHFDFAPAFLIRGEIKKMPAIRFEPALRRQYTPYYFPFTYAPVR"
     gene            2284..2661
                     /locus_tag="PKK76_01640"
     CDS             2284..2661
                     /locus_tag="PKK76_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020762966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNL67190.1"
                     /translation="MASDKSFVQFALDQLKPVSVTCKPMFGEYALYKDNKVVALICDS
                     QLFVKQTEAGKIFAGSIPEAPAYPGAKPSLLATEGLEDPEWICELIRITAKNLPAARP
                     RKKTGAKKKESRANVAAKKKRSK"
     gene            complement(2698..3777)
                     /locus_tag="PKK76_01645"
     CDS             complement(2698..3777)
                     /locus_tag="PKK76_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HNL67191.1"
                     /translation="MSNKLFSPFSARGLDLANRMVMAPMTRNRADGNVPNDLMAEYYG
                     QRAGAGLIVTEGTSPSPNGLGYPRIPGSFNQAQADGWKKVTSAVHAKGGKIFQQMMHT
                     GRIGHQANLPAGAKIVAPSAVAAAGQIYTDSQGMQAHPVPHALSTEEVKSTVQEYVTA
                     AKFAMAAGFDGVELHAANGYLIEQFIHPHSNRRTDEYGGSIENRLRFLVEVASAVAAA
                     IGKDKVGVRLSPYGVFNDLPPYSEVEETYTLAAKKLQEVGILYIHIVDHSSMGAPAVS
                     DSVKASIRKEFQGIVILSGGYDVARANADIDAGKGDLIAFGKQFLSNPDLVVRLEKGK
                     ALNAFDMGTFYTPGPKGYIDYPVAG"
     gene            3905..4174
                     /locus_tag="PKK76_01650"
     CDS             3905..4174
                     /locus_tag="PKK76_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67192.1"
                     /translation="MNGEMEKTEKGSARVDAIKEQAAQLAGKAKEGFARLNERTQVYQ
                     EGAREFLDSVGLYIKENPQRSAIIAVLAGLGLGLLIGRATKRGRD"
     gene            4178..4555
                     /locus_tag="PKK76_01655"
     CDS             4178..4555
                     /locus_tag="PKK76_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67193.1"
                     /translation="MSGKYPALARLRKGQKSAVEEQSESPSLFSSLFTRIHEQWKIRS
                     ARFLAGILLFVSSILLFVISIVFLLGGLYATIKLFVGQIAALFVMGLISLLSGTILLR
                     RSLRNLDESVDFSKKELDLKITD"
     gene            4557..5261
                     /locus_tag="PKK76_01660"
     CDS             4557..5261
                     /locus_tag="PKK76_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNL67194.1"
                     /translation="MQYYRLLGLNQGAGPDEIKNAFRSRIKECHPDRVPGDGEKARRI
                     IEAYRGLLKGETGPEPVAAPVRPSPPPRPQGPTIRVHAKSRSHAAGVEAGRRIFRSVF
                     RGHDLAGSVENFWDAVERHVLEEGEETEVYGKHVHVREVPWEQRLGGNGITNPKAREI
                     FERAEVALREVVAKYDAQKQRSRRQWSKDYIFNLAQVQVLFRDAMNRHPSLTGRALSR
                     LQQIHELIGEIRKMMV"
     gene            5265..5462
                     /locus_tag="PKK76_01665"
     CDS             5265..5462
                     /locus_tag="PKK76_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HNL67195.1"
                     /translation="MNTTIKVEGMTCQHCVKAVEKAVSEKGGTARVDLKSGMVELQFE
                     ESKSSLADIKAAIEETGYSVP"
     gene            5512..6027
                     /gene="msrA"
                     /locus_tag="PKK76_01670"
     CDS             5512..6027
                     /gene="msrA"
                     /locus_tag="PKK76_01670"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNL67196.1"
                     /translation="MSNLQLATVGGGCFWCLEAVFQRVNGVSKVVSGYAGGRTENPNY
                     REVCGGNTGHAEVIQIEFDPATISYEKLLDVFWKCHDPTTLNRQGADTGTQYRSIILT
                     HDENQKKLAIASRTAAEKNFDSPIVTEIVPLTKFYAAENYHQDYYNQNGHAPYCQVVI
                     APKLKKMGMAP"
     gene            6027..7115
                     /locus_tag="PKK76_01675"
     CDS             6027..7115
                     /locus_tag="PKK76_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNL67197.1"
                     /translation="MSRIAFAHTALPKNVYNQDELIGAFRELWAEKFFNTDRLEQFHR
                     NVMVGQRHLALPIEQYRKLSGFGATNSAYIDAAVELSERLLRELFEKTGFDPRTLNAL
                     ISTSVTGIMVPSIDARLMNRLPLPASLKRMPLFGLGCMGGAAGIARAADYLRGHPKDS
                     VVFLSVELCSLTLQREDLSVANIVSSGLFGDGAAAVLMLGEEHPMAGRITPGMPEKAF
                     RSPTILSNRSIFFPNTERVMGWDIVDSGFQVVLDSSVPEFAEKNLRPGVEAFLKEQGL
                     SLSDIEVWIAHPGGPKVITGMEKGLSLREGTLKLSNESLLKYGNMSSASVLFILKETL
                     DQNPPEKKLGLLLAMGPAFAAEAVLLRW"
     gene            7118..7627
                     /locus_tag="PKK76_01680"
     CDS             7118..7627
                     /locus_tag="PKK76_01680"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNL67198.1"
                     /translation="MDSRVLYSAFLVILAIQRLFELRLSKRNATRAFLEGAKEAAPHQ
                     VPWMTAMHSLWFVSCAAEVWMGASFHPGIFILSLAVTVIGQTLRYAAIRTLGPRWNVR
                     IIHFPGKPPVQTGIYRLVRHPNYVGVILEIAAVPMLHGAWITSIAFSIINGIFLYFRI
                     RAEENAVYG"
     gene            7620..7877
                     /locus_tag="PKK76_01685"
     CDS             7620..7877
                     /locus_tag="PKK76_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNL67199.1"
                     /translation="MDETTQDIFLLVKRILREKCKVEGQVGIDARLAEDLRLDSVGML
                     TLALEIENHYRMNLGDEPDSPPRTVQEIVTLIKARLSERKS"
     gene            7874..9523
                     /locus_tag="PKK76_01690"
     CDS             7874..9523
                     /locus_tag="PKK76_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNL67200.1"
                     /translation="MNTLIDALSEAASTTRGIQFFDRSGSITRFNYSELYADAQKIAR
                     VMAQHGLAPGQSAGLVFSTSYEFVRQLFGVLLAGGVPFCLAPPRLGRAADYSEYTARM
                     INSAGARFVIHEDSIGGSLAKVAARTSSPFIPVSQIVADIASASPETKSVTRDRPALI
                     QFSSGTTVDPKPVVLTHGNLVSNTKSILERFPGDINDHSGLSWLPMHHDMGLIGGLFS
                     AVMGKGDITFLRPEDFVMRPALWLRALSETKATTCPCPNFALKLCTDRIETEDVKDLD
                     LSHWQIALVGAETVHLSTLRDFAEKFAITKFRYAAFTPVYGLAEATLAVTFSDLSSPP
                     HAERFDSALLSQGKAVETSETGGSLEIASVGKPLSGFELQIRDDSGAEAQEGQVGRIF
                     LRGESIMQGYLGQPPHEGWLDTGDLGFIHKGDLYIYGRAKDVIIINGRNHDPAYIEFA
                     TEGVPGIRHERTAAFAVSSDTRATEGFILLVEKEKGDLRNQEEVVTLVRDSVTEKTGL
                     IPDSVHIIEIGQMPRTTSGKVRRAEARRQFLSGKIAALASS"
     gene            9559..10740
                     /locus_tag="PKK76_01695"
     CDS             9559..10740
                     /locus_tag="PKK76_01695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNL67201.1"
                     /translation="MIALREFYDAVIVGGGPAGLATAIVLGKENLSVLLIDRRNLPAD
                     KPCGEGLLPPGLEILHDLGVNIPEHMCHPIRGIRYISGNIQAQAEFKEGSGRGIRRTV
                     LSAALLARANEFENVQILSPARAEFPSGLDHFDGTVRISQNNSSAICRTNLIIGADGL
                     HSEIRRLAKLERPGRAKTRFGLRKHFQSNHTLDDFVTVFVAKGSEAYLTPVGQDSFGV
                     AILFSRNEDTRSGKEEAFEALRNRFPGLAPLTQTSAGEQGGAGPLEQRTTGVARDGVI
                     LVGDAAGYLDAATGEGISLALATAQSTARLAQMLRKSNKPLNRLQLSGYEKDHHRITW
                     NYFVMTKMLLFMSDHPTLARPFIRIMAVRPRSFQALLSFNMGEPLFPVLRRFIAGLFR
                     K"
     gene            complement(10748..12028)
                     /locus_tag="PKK76_01700"
     CDS             complement(10748..12028)
                     /locus_tag="PKK76_01700"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL67202.1"
                     /translation="MSETLDRLLETRERRGLRFILTFRSVFVFFGLILAFFTPATRWE
                     MFVTIGLFSSFAICTLSGFYLVRRPSLMSVVGWAGVAIDLVMIAVLPFVWLNSVGGGA
                     VVSRAYLLKLDTLTTMSYSIIVMNSVASRPAYPAATTIGAVAYHLFLFYYAGLDSRVV
                     FEENYVAHMLGDRVHPGLLTTNLLVMAGVGACLTFFTHVSRQTLFEAIHREKAALQIS
                     RYFSPSVYNHIAQAEDSFFTSSGRLQEVTVLFSDLRGFTAMSEKMPAADVIRFLREYH
                     ETMVQIIFKHNGTLDKFIGDAIMATFGTPDTAPDDTNRAVAAALEMRGALTQMNANRE
                     SKGLLPLRQGIGIHTGQVIAGNIGTAERLEYTVIGDTVNVASRVESATKEAKTDILVT
                     QSVADRASALAQFEDAGFFDLKGKAEKIRLFRLL"
     gene            complement(12025..13047)
                     /locus_tag="PKK76_01705"
     CDS             complement(12025..13047)
                     /locus_tag="PKK76_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67203.1"
                     /translation="MSDPILIAINVGITAPNSHNTQPWKFRIISPLEMILYVDEKRIL
                     PVTDPPVRQVHISQGTFLELLSIGASQLGYSAKIELFPEGTYPSIEPGKKPVAHVQLV
                     KDASAPDSLYSMVAERHTNRAEYEGPMISPAEIEKVEADAGAGYTKRVAIYGEDMKPY
                     LPLMNSAMELETMTYRLSDETRGWFRFSDREIQEKRDGLGLPDQGVTGFTRWMIETFF
                     LSREPEKFHDPANNKLFLERYKKKIDSARALYYWSTSSNTIVDWVKTGRDYARFQLAL
                     MKAGLRMHPLSQILQEFPEMDDLRAKFEKISGVRAPGKIQMIVRIGRSDYKYFTPRRS
                     AKDMIV"
     gene            complement(13185..13508)
                     /locus_tag="PKK76_01710"
     CDS             complement(13185..13508)
                     /locus_tag="PKK76_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67204.1"
                     /translation="MLRKTRQLTVPFLILFATAAYAGKQDVTIVNKVKKSIHHVYISE
                     AKVDDWEEDILDDDVLDPGESVKVTFDNSHKACKWDFKAVDGDGVEYLLMGVNLCEVS
                     TINIR"
BASE COUNT         3613 a         3467 c         3386 g         3189 t
ORIGIN      
        1 gaaaggcctg atgcaaacaa gtatgcgatg aaccacatca tgtgtacctt cgcatgaccc
       61 ggccattcgg ggttctgaag gtgagatact ccggaatcac gcagcgcagg gccaaaaaac
      121 tccgcaacgc cgagcgtaag tagaatcctc acaggtagaa atagcttttc gttcatgttt
      181 gatcactccg tatgctgcat catagcgcat ccagaaaatc gagtcgactc cgcttgcaag
      241 ctgagactca aaaagttcag ggtgatgtga tttttttttg agcgaaagcg cggtactgcc
      301 ctggagtcat tcccgtgtgc tctttgaaaa cgcgattgaa tgcagccttg gattgaaagc
      361 cggacatgaa cccggcatcc agaagcgtgc gctccgcaaa ttccgcagac gaaaggatcg
      421 cctttgcagc tgcaatccga tagctgttga taaagccaaa gaagttttgc tttttttcga
      481 tattgatcac catagagaga tggtgaacag gaatagagag ggattcagaa atatccgaca
      541 gggaaatcct ctgatcgagg tagggcttct ctcttaccat gtattcttct agccttcgca
      601 tgtgctcgag acgatccgca tctgccaggt tttgcctcgc gtatcgcacg ttcgaggtct
      661 gttccatccg cagagcaaac acctgtggct tctttatata gtaaaacaat accaggtaag
      721 ccattgctac aatgacgatc ccttccgccg gtgtcgcggg caaagaaaat ccggcaatcc
      781 aggccgcgac cgtgcttgtg aaatacaaaa caaaaaacaa gagcgtgctg ccaaaaagac
      841 tcttcagcca gagaacctga tcacgtacac ttccagcaaa atgctgtgcc acctcagctt
      901 catatcgcga aacaagcagg agtgcacgca cgatgaatgc gccgttgtaa atgcaggaag
      961 taaagatcaa tgatcgtgtg tatactgtaa caaagccaac ctgcaattgt acatcactca
     1021 tgcttcgcat ttctggaaag acagaactca aaacagtatg agacgcaaat acgaacagag
     1081 tcacggcact gaacgcgtag atttcaaatc tgcctggcgg cgatccgtag agagtgtgac
     1141 gcgcgtacag atagagaaga gggcccacca gaaaaccaga gggaaagaca agaaacggca
     1201 gaatgagaag aagtggattg gcagtgcgaa cactgaaacg cagcagactc gccagtgcgg
     1261 caatgaagac gacacccgtg agatagaaat tcccggcacc cgtcctcaat tcgtttaaga
     1321 gcaagatgct caaaaacgca agaaacgcaa gactgatcac cgaaatatat agggatgctt
     1381 cttccaaacg aaactcgcga aaagactgcg aacagaatca tcgaaccggc gcgtatgtaa
     1441 agggaaagta atacggagtg tattgcctgc gaagagccgg ttcaaatcgg atggctggca
     1501 ttttctttat ctctccgcga atcaaaaaag caggcgcaaa gtcgaaatgg tctgtctgcg
     1561 cgtagatgat gcctggaagc cagatttcat acgtcctgtc agaattgatc ttgaatgctc
     1621 gcggccagga tgaatccgca ccgtaatatt ctagaattga atctacgccc tgatgaaaat
     1681 tgtactccgt tatgcctcgc ggcttttgca caccagcggg cgaaagcgcg gcaagactgt
     1741 ctgagagtag gtttacgtct gcattcaact tgcctgcgtc cagaatgcgc atgtgcagtc
     1801 gcaaatcgta cgtatccagg attggctggt cttctggaat gatcggaatc aggcagggtc
     1861 cgcgcatggt cggatgagtg ggagtcccgg cccagaacag aaggttcatc ccttcaaaag
     1921 aaatccgagc acattccaca gattcatccg gtccaatggc gcatgacgtt attgcagaat
     1981 tggttggcgc gcgaagatag tatccggagt taatgctcga gcattgagta atcaatgaca
     2041 ccaaaaaaat gccgcaaaca agctgctgaa aatggcaggt actttgtaaa ttgtaaaccg
     2101 tgcccatcgc tctcgaggat ttttcgtgaa cgcggtccgt gcaacagaaa aaagaatcgc
     2161 agagacaagc ctctgaatcc ttcaccaacg aatccatgag tgccgcataa cggtaatggg
     2221 aaaaagcagt cattttgatc ttgcagcgcg aggccccttt gcgaaaaaca gcctgggcaa
     2281 cctatggcca gtgataaatc cttcgttcaa ttcgctctag atcaactaaa acctgtctca
     2341 gtcacctgca agcccatgtt cggagaatat gcactctaca aagacaacaa agtcgtcgct
     2401 ctcatatgcg acagccagct ctttgtgaag cagacggaag cggggaaaat ctttgcggga
     2461 tcgatccccg aagcaccggc ctaccctggc gcgaagccga gtctactggc aaccgagggt
     2521 cttgaagatc cagaatggat ctgtgagctg atccgcatca cagcaaaaaa tctaccggct
     2581 gcccgcccgc ggaagaaaac cggtgcgaag aaaaaagaat cccgggccaa tgtcgcagct
     2641 aaaaagaagc gctccaaata gcccggcggt gacggccgta accggccgcc ttccgattca
     2701 acctgctact ggatagtcaa tgtaaccctt tggaccaggt gtgtaaaacg tacccatgtc
     2761 gaaagcattg agcgcttttc ctttttcgag gcgaaccacc agatctggat tggaaagaaa
     2821 ttgttttccg aatgcgatca ggtccccctt gcccgcatcg atgtccgcgt ttgcgcgcgc
     2881 gacatcatac ccgccactca aaatgacgat tccctggaac tcctttcgaa tagaagcctt
     2941 gacgctatca gatacagcag gcgcacccat ggaggaatga tccacgatgt gaatataaag
     3001 aatcccaact tcctggagtt tctttgcagc cagcgtgtac gtttcttcta cttctgagta
     3061 gggaggaaga tcgttgaaca ctccgtaagg tgaaaggcga actccaactt tgtcttttcc
     3121 aatggctgcg gccacagcag aggcaacttc caccagaaaa cgaagtctgt tctcaatcga
     3181 tccaccgtat tcgtcagttc tgcgattgga atgcggatga atgaattgtt cgatcaaata
     3241 tccattcgcc gcatggagct ctacgccgtc gaagccggct gccatggcga acttagctgc
     3301 agtcacgtat tcctgaactg tgctcttcac ttcttcggtg ctcaaggcgt ggggaactgg
     3361 atgtgcctgc attccctggg aatcagtgta gatttgaccg gctgcagcga ctgcagaagg
     3421 tgcgacgatt ttagcgcctg ctggcaaatt tgcctggtgc ccgattctac cggtgtgcat
     3481 catttgctgg aagattttgc cgccttttgc gtgaactgcc gaggtcactt tcttccaacc
     3541 atctgcctgc gcctggttaa aagatcctgg aattcttgga taaccaagtc cattgggaga
     3601 aggagaagtt ccttctgtta caatcaggcc tgcgcctgct ctctggccgt agtattctgc
     3661 catcagatca tttggaacgt ttccatccgc acggtttcga gtcatcggcg ccataaccat
     3721 gcgattggcc aggtcgaggc cacgcgcgga aaaaggagaa aaaagtttgt tgctcatatt
     3781 gcttcaagtt aaaagcaatt gaacggaacg gcaattctat cgtctaaaat aaatgaaaag
     3841 gctcactggt gaatcttcgc tttacatcta aaaagatacc accagtttgc accgggagaa
     3901 aaacatgaac ggcgaaatgg aaaaaacgga aaagggatcc gctcgagtgg acgccatcaa
     3961 agagcaggca gcgcaacttg ctggaaaagc aaaagaaggc ttcgcccgtc tcaatgaacg
     4021 cactcaagtc taccaggaag gagctcgcga atttctcgat tcagtaggac tctacatcaa
     4081 ggaaaacccg cagagatccg caatcatcgc tgtgctggca ggacttgggc tgggactcct
     4141 gataggtcgt gcgaccaaac gcggccgcga ctgatccatg tctggcaaat atccggccct
     4201 cgcgcgcctg cgcaagggcc agaagagcgc agtggaagaa cagtcagaga gtccttcgct
     4261 cttctcgtct ctcttcacaa gaatccatga acagtggaag attcgctccg cgcgatttct
     4321 cgcaggcata ctgctttttg tgtcctcgat cctcctattc gtaatctcga tcgtattttt
     4381 gctcggcggc ctttacgcga ccatcaagct ctttgtcgga caaattgcag cgctttttgt
     4441 catgggattg atttctctgc tttcaggaac gattctactt cgtcgttcgc ttcggaattt
     4501 ggacgaatcc gtcgacttta gtaagaagga attggacctc aagattacag actgagatgc
     4561 aatactatag actactaggg ctcaatcagg gcgccggacc ggatgaaatc aagaatgcat
     4621 tccgttccag gatcaaagaa tgccatcctg accgagtacc tggagacggt gaaaaagcaa
     4681 gacgcatcat tgaagcatac agaggtcttc tgaagggaga aacaggacca gagcctgtcg
     4741 cggctccagt gagaccttct cctcctccgc gaccccaggg cccaaccatt cgcgttcacg
     4801 caaaaagccg cagccacgca gcaggcgtag aagcaggaag aagaatcttc cgatccgtat
     4861 ttcgcggaca cgacctggca ggttcggtag aaaatttctg ggatgcagtc gaacgccacg
     4921 tactcgaaga aggcgaagag acagaagtat acggaaagca cgttcacgta cgcgaagtcc
     4981 cgtgggaaca aagactcggc ggaaacggaa ttacaaatcc aaaagcacgc gaaatttttg
     5041 aacgagcaga agtagctcta cgcgaagtcg ttgcgaaata tgatgcacag aagcaaagaa
     5101 gtcgccgcca gtggtccaaa gactatatat tcaatcttgc gcaggtgcag gttttgttta
     5161 gagatgcgat gaatcgccat ccttcgctca ccggaagggc tctcagcaga ctgcagcaaa
     5221 tccacgaact gatcggcgaa attcgaaaga tgatggtttg aacaatgaat actacaatca
     5281 aagtcgaagg catgacctgc cagcattgcg tgaaagcagt tgaaaaagcg gtctctgaaa
     5341 agggcggcac tgccagagta gatttgaaaa gtggaatggt agaacttcaa ttcgaagaat
     5401 ccaaaagcag tctggcggat atcaaagctg ccattgaaga aaccggttat tctgttccct
     5461 gatcctggat tcggagtgac gagtccagcc cgccctgatt tcttataatt catgagcaat
     5521 ctacagctgg ccacagtcgg aggcggatgc ttctggtgtc tggaggcggt gttccaacgt
     5581 gtaaacggcg ttagcaaggt tgtgtctgga tacgctggag gcagaacgga gaatccaaat
     5641 taccgcgaag tgtgcggcgg aaatacaggc catgcggaag tcattcaaat cgaattcgat
     5701 ccagccacaa tctcctacga gaagctgctc gatgtattct ggaaatgtca tgatcctacc
     5761 actctcaatc gacagggagc agacacagga acccaatacc gctccatcat tctcactcac
     5821 gacgaaaatc aaaagaaact agccatcgcg tcacgaactg cagcagaaaa aaatttcgat
     5881 tcacccattg tgactgagat tgttccgctc acgaagttct acgccgccga gaattatcac
     5941 caggactatt acaaccagaa cggacatgcg ccttactgcc aggtagtgat cgcacccaag
     6001 ctgaagaaaa tgggcatggc accctgatgt ctcgaatcgc tttcgcgcac actgctctac
     6061 ccaaaaacgt ttacaatcag gatgaactga tcggtgcctt tcgcgaacta tgggcggaga
     6121 agttttttaa cacagacaga ctcgaacaat ttcatcgcaa tgtaatggtg ggacagcgtc
     6181 atctcgcatt gcctattgag cagtatagaa aactgagtgg ctttggtgct acaaacagtg
     6241 catacataga cgcagctgtt gaactctcag aaagacttct acgagaactg tttgaaaaga
     6301 caggctttga ccccagaact ttaaacgcac tcatttccac cagtgttacc ggcatcatgg
     6361 ttccgtctat cgacgcaagg ctgatgaatc gtctgcctct tcctgcttca ctgaaaagga
     6421 tgccgttatt cggccttggt tgcatgggtg gagcggccgg catcgcacgc gctgcagact
     6481 accttcgcgg acatccaaaa gattccgttg tgttcttgtc cgtagaactc tgttcgctca
     6541 ctctacagag agaagacctc tctgttgcga atattgtttc gagcggcctt tttggagacg
     6601 gcgctgccgc cgtgttgatg ctcggagaag aacatccgat ggcggggaga atcactcctg
     6661 gaatgcctga aaaagcattt cgatctccaa cgattctctc taatagaagc atctttttcc
     6721 caaacacgga acgagtgatg ggctgggata ttgtggacag cggattccag gtcgttttgg
     6781 attcgtccgt tcctgaattc gcggaaaaga atttaaggcc cggagtggaa gcgttcctca
     6841 aggaacaggg gctttctctt tcagacatag aagtgtggat cgctcatccg ggtggaccca
     6901 aagtaatcac cggtatggaa aagggactct cactgcgcga aggaactctc aagctttcca
     6961 atgaaagtct tctcaaatat ggcaacatgt cttccgcctc tgtgcttttc attctcaaag
     7021 agacactcga tcagaatcct ccggaaaaga aactggggct tctcctggct atgggtccgg
     7081 ccttcgcagc ggaagcagtc ctgttgcgat ggtgagcatg gattccagag tactctatag
     7141 cgccttcctt gtgatcctcg ccatacagcg cctgttcgaa ctcagattga gtaagagaaa
     7201 tgcaaccagg gcttttctgg aaggagcaaa agaagcagct ccccatcagg tcccgtggat
     7261 gacggccatg cattcgctct ggtttgtttc ctgtgctgcg gaagtctgga tgggtgcatc
     7321 cttccacccg ggtattttca ttctgtcact ggctgtcact gtgattggac aaactctacg
     7381 ttatgcggcg attcgcacgc tcgggcctag atggaatgtg cgcatcattc attttcctgg
     7441 aaagcctcca gtccagacag gaatttatcg cctggtgcgt cacccgaact atgtgggagt
     7501 gatccttgaa attgcggctg ttcccatgtt gcacggcgcg tggatcacat ccattgcctt
     7561 ttctatcata aatggaattt tcttgtactt cagaatcagg gcagaggaaa atgcagtata
     7621 tggatgaaac gactcaggac atcttcctgc tcgtaaagcg gatcctgcgc gaaaaatgca
     7681 aagtggaagg ccaggttgga atagacgcga ggcttgcgga agatctgaga ctcgattccg
     7741 ttggaatgct cactcttgcg ctcgaaattg agaaccacta tcgtatgaat cttggcgatg
     7801 agccagatag cccgccgcga accgtccagg aaattgtaac tctaatcaaa gcaagattga
     7861 gcgagagaaa atcttgaaca ctctcattga tgccctgtct gaagccgctt ccacaacgcg
     7921 tggaattcaa ttctttgatc gatccggaag catcacgcga ttcaattatt ccgagctcta
     7981 tgcagacgcg caaaagatcg cacgcgtgat ggctcaacat ggacttgctc caggtcaaag
     8041 cgcagggctt gtattttcca cttcttacga attcgttaga caactgtttg gtgttctgct
     8101 ggcgggcggc gtgcctttct gcctagcacc tccgagactg gggagagcag cagactactc
     8161 tgagtacact gctagaatga tcaactccgc aggtgctcgt tttgtcattc atgaagattc
     8221 aattggtgga tcactggcaa aagtagcggc ccgcacttct tctccgttta ttccggtttc
     8281 gcagatcgtc gcggacattg cttctgcgtc tcctgaaacc aagagcgtca cgcgggatcg
     8341 acccgcactc attcaatttt caagcggcac gactgtagat ccaaagcccg ttgttttgac
     8401 tcacggaaac ctggtttcaa atacgaaatc aattctggaa agatttccag gagatataaa
     8461 cgatcacagc ggcctgtcct ggttgcccat gcatcacgat atgggcctca ttggcggatt
     8521 attttcagca gtcatgggaa aaggagacat cacgtttctt cgccctgaag attttgtaat
     8581 gagacctgcc ctctggttgc gcgcactcag cgaaacaaaa gcaaccacat gcccctgtcc
     8641 caatttcgcg ctcaagctat gcacggatag aattgaaaca gaagatgtaa aagacctgga
     8701 tctttcccac tggcaaattg ctctcgtagg agcagaaaca gttcacctct ctactttgcg
     8761 agacttcgct gaaaaattcg caatcacaaa gttccgttat gcggcattca caccggtcta
     8821 tggtcttgcg gaagcaactc ttgcagtcac attctccgat ctatccagtc ctccgcatgc
     8881 agagcgattt gattctgctc ttctttcgca gggcaaagca gtcgaaacaa gcgagactgg
     8941 cggaagcctt gagattgcat ctgtgggaaa accactgagc ggatttgagc tccaaatccg
     9001 cgacgattca ggagcagagg cgcaagaagg ccaggtaggc agaatctttt tgcgcggaga
     9061 atccatcatg caggggtacc tgggacaacc gcctcatgaa gggtggctgg acacggggga
     9121 tcttggtttt attcacaagg gagacctcta catttatggc cgcgcaaagg atgtaatcat
     9181 catcaacgga agaaatcatg atcctgctta tatcgaattc gcgacagaag gagttccagg
     9241 aattcgccat gagcgaaccg ctgcctttgc agtttcatcc gacactcgcg caacggaggg
     9301 atttatcctg cttgtggaaa aggaaaaagg agatctgcga aatcaagaag aggtcgtgac
     9361 tcttgtgcgc gattctgtta cagagaagac cgggctcatc cctgattcag tacacatcat
     9421 tgaaataggg caaatgccta gaaccaccag cggcaaggta aggcgcgcag aagccagacg
     9481 gcagttctta tccggaaaga tcgcggctct cgcatcgagc tgattttgcc cgcatgaagc
     9541 agtgatgcgt agaaaactat gatcgccctt cgcgaattct acgacgcggt cattgtaggc
     9601 ggtggacctg ccggacttgc aacggccatc gtcctcggga aagaaaacct ttccgtgctc
     9661 ctcatagaca ggcggaacct tccggcggac aaaccatgcg gagaaggcct tcttccgccc
     9721 gggcttgaaa ttctacatga tcttggagtg aacattccgg aacacatgtg tcacccgatc
     9781 cgcggaatcc gctacatctc cggaaacatc caggcacagg cagaattcaa agaagggtca
     9841 ggaaggggaa ttcgcagaac ggtgctctcc gcagcgttgc tcgcacgcgc gaacgaattt
     9901 gaaaacgtcc agattctctc acccgctcgc gcagaatttc cgtcaggcct ggaccatttc
     9961 gacggcacag tgcgcatttc gcagaacaac tcaagcgcaa tctgtcgcac aaatctgatc
    10021 ataggagcag acggccttca ttcggagatc cgaagactgg caaaactcga gcgccccgga
    10081 agagcgaaga ctcgcttcgg gctgcgaaag catttccagt ccaatcatac gttagacgac
    10141 tttgtgactg tatttgtagc caaaggttcc gaggcctatc taacacctgt cggccaggat
    10201 tcattcggtg tggccatcct gttttccaga aatgaagata cgagatcggg caaagaagag
    10261 gcgtttgagg cacttcgaaa tcgattcccc ggacttgcac ccctcactca gacttcagca
    10321 ggagaacagg gcggcgccgg gcctctcgaa caaaggacaa caggggttgc gcgcgatgga
    10381 gtgattctgg tgggagatgc cgcaggatat ctggacgcgg ccacaggaga aggaatcagc
    10441 cttgccctgg ccactgcaca atcgacggcc cggctcgcac aaatgctacg aaaatcaaac
    10501 aagccgttga acagactgca gctgtcgggc tatgagaagg atcatcatcg cataacgtgg
    10561 aactatttcg tgatgacaaa gatgcttctg ttcatgtcgg atcatccgac gctggctcgc
    10621 cctttcattc gcatcatggc agtgcgccct cgttctttcc aggctctact ctctttcaat
    10681 atgggagagc ctttgtttcc agtcctccgg cgatttatcg cgggcctctt tcgcaaataa
    10741 gaacttttta aagaaggcga aagagtctga tcttttctgc ctttcctttc aaatcaaaaa
    10801 aacctgcgtc ttcaaactga gccagcgcag acgcgcgatc cgcaacactc tgcgttacca
    10861 gtatatccgt tttcgcttct ttggtagccg attctactct gctcgcaacg ttgacagtgt
    10921 ccccgatcac agtgtattcc aatctttctg cagttccaat attgcccgca atcacctgtc
    10981 cggtgtgaat ccctattccc tgtcgcagcg gaagcagccc tttcgattca cgattcgcat
    11041 tcatctgtgt gagagctcct cgcatttcga gagcagcggc aacggcacgg ttcgtatcgt
    11101 ctggcgcagt atcgggggtc ccgaacgttg ccataatcgc atccccgata aacttatcca
    11161 gtgtgccatt gtgtttgaaa atgatctgca ccatggtctc gtgatattct ctcaaaaagc
    11221 gtataacgtc tgccgcaggc attttttcgc tcatcgcagt gaaaccgcgc aaatcggaaa
    11281 ataaaaccgt tacttcttgc agtcgcccgg atgatgtaaa aaaggaatct tccgcctggg
    11341 cgatgtgatt atacacggaa ggtgaaaaat atctgctgat ctgaagagcc gccttttcgc
    11401 ggtgaatggc ctcaaacaat gtctgacgag aaacgtgggt gaagaaagtc aaacaggcac
    11461 ccacgccagc cataaccagc agattggtcg tgaggagccc ggggtggact ctgtcgccca
    11521 gcatgtgcgc tacatagttt tcctcgaaaa ccaccctgga atcaaggccg gcgtagtaga
    11581 atagaaacag gtgatacgca accgcgccta ttgtggttgc agccggatac gctggcctgg
    11641 acgcaacgct gttcatgaca atgattgagt acgacatagt ggtaagggta tcgagcttca
    11701 gcaaatacgc gcgcgaaaca acggcacctc cgccaactga attgagccat acgaaaggaa
    11761 gcacggcaat cataacaaga tcaatcgcta cgcctgccca gcccactacg ctcattaaag
    11821 acggtcgccg aaccaggtag aaccctgaga gagtgcagat tgcgaagctt gagaataaac
    11881 caattgtgac gaacatttcc cacctggtcg caggcgtgaa gaatgcgaga atcaaaccga
    11941 aaaaaacaaa tacagagcga aacgttagaa tgaatcgaag cccgcgtcgc tcgcgcgtct
    12001 caagcaatcg atccagtgtc tcactcaaac aatcatgtcc ttcgcgcttc gtcttggagt
    12061 gaaatattta tagtccgatc tgccaatgcg aacaatcatc tgaatttttc caggagcacg
    12121 cacgcctgaa atcttttcaa acttcgcacg caaatcatcc atttccggaa attcctgcaa
    12181 tatctgactg agaggatgca ttcgcaaccc cgccttcatc aatgcgagct ggaagcgagc
    12241 gtaatctctg ccggtcttta cccagtccac gatcgtattg gaacttgtag accaatagta
    12301 aagagcacgt gcagaatcga tcttcttttt gtaccgttcg agaaacagct tgttattcgc
    12361 aggatcatgg aatttttccg gttcacgact cagaaagaat gtttctatca tccagcgcgt
    12421 aaatccagtg actccctgat ccggaagacc cagtccatct ctcttttctt gaatctcacg
    12481 atcgctaaat ctgaaccagc cgcgagtttc atctgaaagg cgatatgtca tggtttcgag
    12541 ttccatggca gaattcatga gaggaagata aggcttcata tcttccccgt agatcgccac
    12601 acgctttgta taccctgcac ccgcgtccgc ctccactttt tctatttcag caggagaaat
    12661 catagggcct tcgtactctg cgcgattcgt gtgacgctcg gcgaccatgg agtacagcga
    12721 atcaggtgca gatgcatcct tgaccaactg cacgtgcgcg acaggcttct tgcctggttc
    12781 tatactcggg taagtaccct ctgggaaaag ttcaatcttc gcagaatatc caagctgcga
    12841 agctccaatg ctgagaagct caaggaaagt tccctgcgaa atgtgaacct gtcttacagg
    12901 aggatctgtg actggtagaa ttctcttctc atccacatac agaatcatct ccagaggaga
    12961 aatgattctg aatttccagg gttgcgtgtt atgcgaattg ggtgctgtga ttccgacgtt
    13021 gatcgcaatc agaatgggat ctgacatacc gttcgatcgc gcattctgaa tgggatctgc
    13081 accgtccggc ccagggagtc gggggtgcgt tccacattgt tcaagaaaaa gaggcaccgt
    13141 aagcagcggc gcgcttgagt agaggaactt tttccggctg atcatcagcg gatgttgata
    13201 gtggacactt cgcacagatt cacacccatg agaagatatt ccacgccgtc tccgtcgacc
    13261 gctttgaaat cccacttaca cgctttgtgg ctattgtcaa aggttacttt cacgctttcg
    13321 cccggatcca gtacatcgtc gtccaggatg tcttcttccc agtcgtctac tttggcttcc
    13381 gaaatataca catggtggat cgatttcttc accttgttta caatggttac atcctgtttg
    13441 ccggcataag ccgcggtggc gaacagaatg agaaatggta ctgtaagttg tcttgttttg
    13501 cggagcattt cgcgagcctc catcgctcag gctcgaaaat caaactctat tttctggttt
    13561 tcaagtggaa tttcgaactg catggcagtt cctacccggt cttcctgagt caggtcaaag
    13621 ggcacacctt cgattctttt ccccttctga gtggc
//
LOCUS       DAORFF010000020        13131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2033_length_13131_cov_4.193484, whole genome shotgun sequence.
ACCESSION   DAORFF010000020 DAORFF010000000
VERSION     DAORFF010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13131
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2033_length_13131_cov_4.193484"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="PKK76_01715"
     CDS             <1..1476
                     /locus_tag="PKK76_01715"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNL67205.1"
                     /translation="FLLFFALTQAAFTAYLICLLKTINTNESQALLFERIENAILPFI
                     AVFFLFFAQRFRPILPDWFVWVFTIADAMLSLIILFYPHAYNSELGAVKTFPALGVTF
                     YETKQPVWVFMFIALNLIGMAGIIVRYLQLGLFRQIESRILVVSLVLFFLMGANDMAV
                     SLEFYTFPYIAHFGFVLVMFAVESLFTFEVKFAYSHAGEAATENNNPPATDDRPDWKK
                     NAEMREEARKERERLREPPEDPDSAEERPLYVRVLGSLEVRLNGVKIAAEELGGKKKL
                     LTLFKILLVRYGKGIHREELLELLWPDLTEANALNNLHALIFRLRKVLTVSEAVAFIS
                     DTVSLDPSLVSTDAARLDERIAESLALLSSHPERAITLMEKATDLYRGDYFEFDPNFE
                     GTQMIRDQIATRFKKSLLKVCSEITKENQSGSLIHFAEKCISLDNLDEECWRQLLRGL
                     ALAGKKNEARRKYDQLKTLLQKELSVLPDAQTDDLMRRIGS"
     gene            1525..2040
                     /locus_tag="PKK76_01720"
     CDS             1525..2040
                     /locus_tag="PKK76_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67206.1"
                     /translation="MRVILLLLAFFAGCSRNPSGSWTMTSEDAGLNGTIELTGQKFFR
                     VRVKSKDVDTAGAGLATAGRVAVCLGTGCRVRVLIEKFGNWRGMATDGLRTTDESWEK
                     SGQDLIGPAGKKPLGGNAMPGPNQFIAELHDQVVLADDAEGLILLDLARDSGRGEIIR
                     PSGKKSITLRR"
     gene            complement(2090..2899)
                     /locus_tag="PKK76_01725"
     CDS             complement(2090..2899)
                     /locus_tag="PKK76_01725"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL67207.1"
                     /translation="MSALREFFVNLKKGTEHLNYGREILRDWGAEHALKLASRDQPIS
                     VYDLGCGHGTDIVNIRQHASIQTTAAFRWQLAGIENFSEYQKECKSLGIATHAVDLER
                     DTYPGKNASVDILIANQVLEHTKEIFWIFSEAVRLVRPGGKFLVGVPNLASLHNRLLL
                     LFGEQPTAQQSLSAHVRGFTKPDFKKFAETGGFFKLTEFRGSNFYPFPPGIARPLARM
                     FPTLAWGAFFNLERTDRRGDFLECLSGDENFLETPFYGSPHNPARGRKLRK"
     gene            2942..3745
                     /locus_tag="PKK76_01730"
     CDS             2942..3745
                     /locus_tag="PKK76_01730"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNL67208.1"
                     /translation="MNIKGKNVLITGASSGIGEAAARETARRGGIPLLVARREEELAR
                     VAREIQTESGIKAAFLVCDVSSPLDRIKIEPFARSAGGVSILINNAGITAHGRFDQTQ
                     PGIAEQAMNLNFFAALYTTQTLLPVMRETAGQKKIVLVSTPSALYGIPGRFAYSASKA
                     AGHAWMESIRIELKREGFDTLIFCPGYTRTQLRTRGLSADGTVLSEEQASGAKDPSQV
                     ATILLEAVRKDKRIAFTNSAGPLVYYLRTLAPGFLEKMLAKKLKKDFQT"
     gene            3772..5037
                     /locus_tag="PKK76_01735"
     CDS             3772..5037
                     /locus_tag="PKK76_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNL67209.1"
                     /translation="MQPFIPSAFNLPVAWAVDFSLPILLKQLQNLDGVVLKPQDVKML
                     RSIRDDRLLYFSNHPTTSEPPVAYYVANVMGARFQFMAARQVFEWGGGLVGKFISSIG
                     AFSVLAGAADRESIKMSRAILSQPRGKLVLYPEGEPTSGENDNLLPFQQGAAQLGIWA
                     LEDARKSDPSADVTVLPTFVKYVMTGTEAHLRADLHQNIKIVEAKLGVDPGNRNLLRR
                     FLAVGRVMLEKAEKDYGVPMASEKDYDYRVGRVRHVILDNVAERLGLTSYDKKSDAIV
                     KLRHLLAVIEMILVKFPDPRLPNPTASDIEWAQRECQKAYDFIVIKPEYLLSRPSAER
                     FYEWLARFETTIDGSTNPRPRKAHVFFAKPFLLSEYYADYKKEKKVTVEKLTARLRAD
                     TQALLDQAVNLTEPLVRPYDIGAPEKPGP"
     gene            5034..6203
                     /locus_tag="PKK76_01740"
     CDS             5034..6203
                     /locus_tag="PKK76_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67210.1"
                     /translation="MRLDPRDPIFLQSPLDLIHKYYIENAIQDYRIYTDRLTNQFCAA
                     APAPVLLSNRARVKDQSFFLRNAEIRARKLTLIAAIDGEEREISGESFGDFLREGENR
                     IAIAQDDLLLQEEYPRQAGLFSYRPDRAKMDIQYRPDFGHGEHVQYFIASTGTPFGVF
                     SPGSFVFIDGVNRHRSQMIYDELLQSKIISRGGMVLDAPRLAGYDFLTPLTDYQAADL
                     KNMLLHPAGLPALEKRYATLLTHEKSEIARDLNGALPHLRAEQLEAHLASMREDERKT
                     LFAFFVSRVVSSLLADQIVRYRGILRDNAKREILILLYEPVHSSSHYYGRLLDFFAKE
                     KPGYNLRLAMAYKRPPPRRRENLLHPFVEALRDLENKKYIESAEPLIPDDSSPRD"
     gene            6237..7955
                     /locus_tag="PKK76_01745"
     CDS             6237..7955
                     /locus_tag="PKK76_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNL67211.1"
                     /translation="MYNTSLALATRAARFRSRLTFGALVFFAFFRLILLPEGEGLPLF
                     SSWLADSAVFLTLLLIARILQAIPGGRIVSRLFFHSAYLLYFVLTFSYCYFFREATIR
                     QYSIFDLTLSGIAYFFTNVLPFWGNILLLAALLLVYVVSYLRDDWHIPTTLAFLLCGS
                     LFMVAKATANENASPILHLIQDVSEAARSPGLTVDPAPTEFSLDDLDSWTHPAPRFEP
                     RFQKIIVLVMEQITLAQLREDSRALPHGTFFNREEEHSHEYNNFFAADMDSRTGMLAL
                     LGGRLIPFEAYSDEDVDQYSFVSGKPSLLNIVKNRNYRTVYSASETERELVVTDLPWD
                     QIYTLTHEEVDQARSKYLCINPYEFEHSCEDKIMVSRLVNDVVKHDRVFLFYEFIFGH
                     ASEYIDIQKKTAVQYYSEFIDDLISGLRKAHELDHTLIVVTSDHGIRDHGYESWLSTY
                     RIPLLFYHSSFKKQLHPELHSQIDFRDIFLEESSGKPSSLKSRKFAVFMGTTTSSIVG
                     AITDQGDVMVLKNRKWLPYVMFNKNADEEDAPGGPPKSDLKPGAVVRYIQDYRAKFLS
                     GNLPAH"
     gene            complement(7952..11236)
                     /locus_tag="PKK76_01750"
     CDS             complement(7952..11236)
                     /locus_tag="PKK76_01750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNL67212.1"
                     /translation="MIFEDPTARRWRSFLIAFFTFLLVGGGLAAAFVAALFVTPPLPD
                     FQHKRNVDKRVRILRDQAQDLSAKQKHGHEVQRKVEDKKGPLLAIPRQGDKLVTAFLV
                     QHDLASVAAFRDHKDRIDVVFPDWYFLTSAKCEVSERIDEEVRRVLDESDSAVIGRVT
                     NGDHEKWHTAEMRRLLASPQLRECIAGVLVQFAERDHLKGLNIDIETLGTAGRADYVK
                     FLKELADRLHERDMILTVDITAHDPAYDVKAIAQVADGVVLMAYDEHYTSGPAGPIAS
                     QEWFEENVAFTLKDVPPEKLIVGIGTYAIDWSQNHSYSMKFSEVMALADDMGALPGMD
                     PESRNMHFSYSEDGNNHTVWFLNNVTAWNQKLAIDRLGVAGIGVWRLGAEDPSIWKIL
                     PQQKPNIEAVVEPEALRSVLYLSFGEVFHLHTVPSRGKMSVEQDGEGFIDAAKYDSLP
                     EGYLIERIGNSIPDKSLVLTFDDGPDPVWTPKVLDALKESKVHATFFVVGEQAERYPE
                     IVQRILAEGHAIGNHTYFHPDLSKTSPERVRLELNSTQRIIERETGYKTMLFRAPYNT
                     DSNPDEAPEIDPLRTAADMGYLIIGANIDVADWQKPTARLMADRLIEQAKNPRNHIIV
                     MHDGGGDRSRTVEALRIGIPELKSKGYVFQSLEDATGISKSTLFPAMPASEAMLVYIT
                     AASWLSIRWGWVIITWLFLFTTLLAIFRIVFLGILVFKSVRKAEERKDDGTFLPPVSV
                     LIPSYNEAKVLSQTIQSLQQSTHPQFEMLVIDDGSTDNTKEIVRGLMKKDKRIRLVSQ
                     PNGGKSSALNLGMKEAKHEIVVTIDADTIVMPQTLTELAAPFKDPLVDAVCGNVEVGN
                     VHNVLTGFQSLEYITSQNFDRRAFDALNCISVVPGATGGWRRSKVLEAGGYKSDTLTE
                     DADLTLTMLENGARIVYAAEARSRTEAPATIRTLSKQRFRWSFGTFQCLWKHRHAFFK
                     GPLGWVALPNMFLFQVLFPALSPIGDVVFILSILRGDMEAIAVGYLLFLFMDVCGSLL
                     AFILENKRKRLMFLVLIQRFFYRQFMYIITFKAMFAMLKGARYGWNKLERQGTVRMNP
                     ELAKSH"
     gene            complement(11396..12484)
                     /locus_tag="PKK76_01755"
     CDS             complement(11396..12484)
                     /locus_tag="PKK76_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HNL67213.1"
                     /translation="MELIKKIFDRRTATIAAVSSIAAVALALGGGIVSADVKTAVGSD
                     VSSNELRSITVESWDKDYSNGGYGWEVSTDKDMTSKDGQYQPNAQSLQAEREVKLIKG
                     TPQDIRENVNYPEARVLGVKFGFTFPGYNVVSIRPPHVDQYVVERPRPFLNEYAVTGD
                     YKSRSCYKNPALSSVNAGRAMVVDCLYGIELPGEVHKVSVWVMGRGNDYDLEGWFEDW
                     KGATHILKFGSVNFIGWRPMSIEIPKSIPQDVNSFPAIKTLVFKQFKLRSKPHTSLES
                     VYIFFDELRVLTDIFEVHFDGAALDFDKADCDRKNHLLKMIRDNARNPDAYTALVDCS
                     KAPGPAAPIQSGNTQPSQPAQPAQPAQP"
     gene            complement(12511..>13131)
                     /locus_tag="PKK76_01760"
     CDS             complement(12511..>13131)
                     /locus_tag="PKK76_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HNL67214.1"
                     /translation="TGGLEVILLDDFEESEDWHARSTCPLGETTTLKMVQRGEIRAAD
                     DEQSKPPEGESLNVQNNPDNPNHILGIKTYFDNRGFDRVEVKPPHEYVIKGKARQFSV
                     WVLGRKFRHNMSLKLRDYRGNTHNLPLGRLDFFGWRKLTVTVPGWLPQSTRYALLDKN
                     LHFVSLFVTSDVHEVPGQFYFYVDGLKALVDKSENAYPGSEVKDNW"
BASE COUNT         3309 a         3455 c         3139 g         3228 t
ORIGIN      
        1 ttcctcctat tcttcgcact cactcaagcc gcattcacag cgtacctgat ctgccttctc
       61 aaaacaatca acacaaatga atctcaggcg cttctattcg agcgcataga aaatgcaatc
      121 ctgccgttca ttgctgtctt cttcctgttc ttcgcgcaaa gattcagacc cattctgccg
      181 gactggtttg tctgggtctt cacgatcgca gatgcaatgc tttctctgat catcctgttt
      241 taccctcacg cgtacaacag cgagctggga gcagtgaaaa catttccagc tctgggtgtc
      301 acattctacg aaacaaaaca accagtatgg gtgttcatgt tcattgcgct caatctaatt
      361 ggaatggccg gcatcatcgt acgatatctc caacttggac tgttcagaca aatagaatcg
      421 cgcatactcg tcgtatcgct cgtcttgttt ttcctgatgg gcgccaatga catggcggtt
      481 tccctggaat tctatacctt cccctacatt gcgcacttcg gatttgttct ggtaatgttt
      541 gcagtagaga gtctttttac attcgaagtg aagttcgcat actcacacgc aggcgaagca
      601 gcaactgaaa ataacaatcc accagccacg gacgatcgcc cggactggaa aaaaaatgct
      661 gaaatgcgcg aagaggcacg caaggaaaga gaacggttgc gtgaacctcc agaagatcct
      721 gattcagcgg aagagcgtcc tctttatgtc cgggtgcttg gctctctcga agtgagattg
      781 aatggtgtga aaatagcggc agaagagctc ggtggaaaga aaaagcttct aacccttttt
      841 aagatcctgc tcgtgcgata tggaaaagga atccaccgcg aagaactgct ggaactattg
      901 tggccggatc taacggaagc caatgctctc aacaatctcc atgccttgat ttttcgctta
      961 cggaaagttc tcaccgtatc ggaagcagtc gcattcatca gcgatactgt ttcccttgat
     1021 ccttctctcg tatccacaga cgccgccaga ctggacgagc gaatcgcgga gtctctcgct
     1081 ttgctttcct cccatccaga acgcgccatc acactgatgg aaaaggcaac ggacctgtat
     1141 cgcggggatt acttcgaatt cgacccgaac ttcgaaggga cacaaatgat ccgtgatcag
     1201 attgcgacca ggttcaagaa gtcattgctg aaggtatgct ccgagatcac gaaagaaaac
     1261 cagtccggtt cgttgattca cttcgcagag aaatgcatct ctctggacaa tctggatgaa
     1321 gaatgctgga gacagctgtt gcgcggactg gccctggcag gaaagaagaa tgaagccagg
     1381 agaaagtatg atcaactcaa aaccctgcta caaaaggaat tgagtgtact gcctgatgcc
     1441 caaacggatg atctcatgcg cagaatcggc tcatgatctt ttgaatctac ttgcatccag
     1501 gatgctggag tgcgcgactt gcgcatgcgt gtaatcctcc ttttacttgc tttcttcgca
     1561 ggctgttctc gaaacccatc ggggtcctgg accatgacgt cggaagacgc cggactgaat
     1621 ggaactatag aactgaccgg tcaaaaattc tttcgggttc gcgtcaaaag caaagatgta
     1681 gacacggcag gtgcaggtct cgccacagcg ggaagagtgg ctgtatgcct gggcacagga
     1741 tgtcgcgtgc gagtactcat agaaaaattc gggaactggc gcgggatggc aacagacggc
     1801 cttcgcacaa cggacgaatc ctgggaaaaa tcaggccagg atctgattgg ccctgcggga
     1861 aaaaaacctc tcggcggaaa tgcgatgccc ggaccgaatc aattcattgc tgagctgcac
     1921 gatcaagtcg tgctcgcaga cgatgcagaa ggactcattc tcctggatct cgctcgagac
     1981 tcggggcgcg gagaaatcat tcggccttca ggaaaaaaat cgattactct gcgacgctaa
     2041 cgcagcagtg atccagtatt tgcgaaacac aaacggggaa cacaaaccat tacttacgca
     2101 attttcttcc gcgcgcaggg ttgtgtggac tcccatagaa tggcgtttcc agaaaattct
     2161 catctccact caagcattcc agaaaatccc cgcgacgatc tgttctctcc agattgaaaa
     2221 acgctcccca cgcaagagtg ggaaacatac gcgccagagg tctggcaatg ccgggcggaa
     2281 atggataaaa attggacccg cgaaattcag tgagcttaaa gaatccgccc gtctccgcaa
     2341 attttttgaa atcaggtttc gtaaatccgc gaacgtgcgc gctcagactt tgctgggcgg
     2401 ttggttgctc cccgaacaga agcaacaaac gattgtggag actcgcaaga ttgggaacgc
     2461 ctactagaaa ttttccccct ggacgaacca gacgaactgc ttctgaaaag atccagaaga
     2521 tttcctttgt atgctccagc acttgattgg caataagtat gtccacagac gcatttttac
     2581 caggatatgt gtccctttca agatctactg catgcgtcgc gatccccaat gatttgcatt
     2641 ctttctgata ctcactgaag ttttcaatcc ctgccagctg ccagcgaaat gcggcagttg
     2701 tttgaatgct cgcatgctgg cgaatgttca caatgtccgt tccgtgtcca caacccagat
     2761 cgtacacgct gattggctga tcacgagaag ccagtttcag tgcatgctcc gcgccccaat
     2821 cgcgcagaat ttctcggcca tagttgaggt gctccgttcc tttttttaga tttacaaaga
     2881 attcgcgaag ggccgacata tctccgcata ctgtgccggg gtctacggtc aaattgaatc
     2941 catgaacatc aaagggaaaa acgtactgat cactggagcc tcgagcggaa tcggagaagc
     3001 tgctgccagg gaaacagcca gacggggcgg aatcccccta ctggttgcaa gacgcgaaga
     3061 agagctggca agagtggcgc gcgaaatcca aaccgagagc ggaatcaaag cagcgttcct
     3121 cgtatgcgat gtttcttctc ctctcgacag aatcaagatc gaaccgttcg cacgatctgc
     3181 cggaggtgta tctatcctga tcaacaatgc tgggatcacc gcgcatggta gattcgatca
     3241 aacacaaccg ggcattgcag aacaagcaat gaacctcaac ttttttgccg cactttacac
     3301 tacacagacg ctactaccgg tgatgagaga aactgcgggt cagaagaaaa tcgttctggt
     3361 gtccacaccc tccgccctct acgggattcc gggcagattc gcctacagcg caagcaaggc
     3421 ggcaggccac gcctggatgg aaagcattcg cattgaattg aaacgcgaag gctttgacac
     3481 tctcattttt tgtccaggtt acactcgcac acaacttcgc acgcgtgggc ttagcgcaga
     3541 cggaactgta ctctctgagg aacaggcgtc gggagcaaag gatccttctc aggtagccac
     3601 catccttctt gaagcagtac gcaaagacaa acgcatcgct ttcacaaaca gtgccggtcc
     3661 actggtgtac tatctgcgca cgctggcccc cggattcctc gaaaagatgc tggcaaaaaa
     3721 actaaaaaaa gactttcaaa cttagtgtac gccggaagat tttctctgta gatgcaacca
     3781 ttcataccgt cggctttcaa tctccctgtt gcctgggccg tagactttag ccttcccatt
     3841 ttactcaagc aattgcagaa tctagatggt gtggtcctca agccgcagga tgtgaagatg
     3901 ctgcgctcga ttcgcgatga tcgtctgctc tatttcagca atcacccgac tacttctgaa
     3961 ccgccggttg cgtactacgt ggccaatgta atgggagctc gctttcaatt catggccgcc
     4021 cgccaggtat ttgaatgggg cggtggtctt gtggggaagt tcatttccag cattggagca
     4081 ttctcagtac tcgcaggcgc ggcagatcgc gagtccatca agatgtcacg cgcaatcctc
     4141 agccagccgc gcggaaaact ggttctatat ccggagggag aacccacaag cggggaaaat
     4201 gacaatctac ttccgttcca gcagggtgcc gcacagctcg gtatctgggc actggaagac
     4261 gcgcgaaaat cagatccatc cgcggatgtt acagtcctcc ccacgtttgt aaaatacgta
     4321 atgaccggaa cggaagctca tctccgagca gatcttcatc agaacatcaa gatcgtagaa
     4381 gcaaaactgg gagtggatcc aggaaacaga aatttactgc ggcgattcct cgctgtgggt
     4441 cgcgtgatgc ttgaaaaagc ggaaaaagat tacggcgtcc ctatggcatc agagaaagac
     4501 tacgactacc gcgtggggcg tgttcgccat gtaatcctgg acaacgttgc agagagactc
     4561 ggcctcacct cttatgataa aaagagtgac gcgattgtca aactgcgcca tcttctcgca
     4621 gtcatcgaaa tgattctggt caaatttcca gacccacgcc tgcccaatcc caccgcgagc
     4681 gatatcgaat gggcacagcg cgaatgccag aaagcatatg acttcattgt aatcaaaccc
     4741 gagtatctgc tttcgcgacc ttccgcagag agattctacg aatggctggc acgattcgaa
     4801 acaaccattg acggatccac aaatccgcga cccagaaagg ctcatgtatt cttcgcaaag
     4861 ccctttcttc tatctgaata ctatgcggac tacaagaaag aaaagaaggt taccgtggag
     4921 aaactgactg cccggcttcg tgcggacacg caggcactcc tggatcaggc cgtcaatctg
     4981 actgagcccc tggtacgtcc gtacgacatt ggcgcgccag aaaaaccagg cccatgagac
     5041 tggatccgcg agatccaatc ttcttacagt cgcctctgga tctaattcat aagtattaca
     5101 tagaaaatgc gattcaggac tatcgcattt atacagacag actcacaaac caattctgtg
     5161 cagccgctcc cgcaccggtt ttactatcca atcgtgcccg cgtcaaagac caatctttct
     5221 tccttcgcaa cgcagaaatc cgggcacgca agttgaccct catcgcggcg attgatggag
     5281 aagaaagaga aatatccgga gaatcgttcg gtgatttttt gcgcgaaggg gaaaacagaa
     5341 tcgccatcgc ccaggatgat cttctactgc aagaagagta tccacgccag gcaggcctgt
     5401 tcagctacag gccagatcgg gcaaagatgg atatacagta tcgtcccgat ttcggtcacg
     5461 gagaacacgt tcaatatttc attgcttcta ctggcacgcc gttcggagtt tttagtccag
     5521 gcagctttgt gtttatagac ggagtcaatc gtcatagatc tcagatgatt tatgatgaat
     5581 tgctccaatc taaaatcata agccgtggag ggatggtcct ggacgcgcca agacttgcag
     5641 gatacgactt tctaacaccc cttacagatt accaggcggc agatctcaag aatatgctcc
     5701 tccatcccgc cgggcttccc gctcttgaga aacgatatgc gacattgctc actcacgaaa
     5761 aatcagagat cgcacgggat ttgaacggag cgttgccgca tctacgcgcc gaacagctcg
     5821 aggcacatct ggcctccatg cgtgaagatg aacgaaagac tctatttgct ttctttgttt
     5881 ctcgcgtcgt ctcatctctg ctcgcggacc agatcgttcg ctacaggggc attctgcggg
     5941 acaatgcaaa gcgagaaatt ctcatcctgc tttacgaacc ggtccactcc tcctctcact
     6001 actatggacg actgctggac ttttttgcaa aagaaaagcc cggctacaac ctgcgtctgg
     6061 ccatggctta caaaagaccg cctccgcgaa gaagagagaa cctgcttcat ccgttcgtag
     6121 aggcactgag agacctggaa aataagaagt acattgaatc tgcagagcct ctgattccgg
     6181 acgatagttc acctagagat tgaccgccat acggcatccg tcctagatcg ctttaaatgt
     6241 ataacacatc cctggcactg gcaacccgag ccgcgcgctt tcgctctcga ctcacgtttg
     6301 gagcccttgt tttctttgca ttcttccgac tcattctgct gcctgaaggg gaagggcttc
     6361 ctctcttctc ttcgtggctt gcggactcgg ctgtcttcct gactcttctt ctgattgctc
     6421 ggattttgca ggccattcca ggcggccgaa ttgtttcccg gctgttcttt cactccgcgt
     6481 atcttcttta cttcgttcta actttttcct actgctactt ttttcgagaa gcaaccatta
     6541 gacagtattc catcttcgat ttaacgttat ctggcattgc ttacttcttt accaatgtgc
     6601 ttcctttctg gggaaacatt ttgcttctcg cagccctgct tctggtctat gtggtgagct
     6661 atctcagaga tgactggcat attccaacca ccctcgcttt cctattatgc ggaagcctct
     6721 tcatggttgc aaaggctacg gcgaatgaga atgcttcacc cattcttcat ttgatccagg
     6781 acgtctcgga ggctgcgcgt tctcccgggc tcacagtaga tccggctccc actgaattct
     6841 ctctggacga cctggactca tggactcacc ccgcaccgcg cttcgaaccg cggtttcaaa
     6901 agatcatcgt gctggtgatg gaacagatta ctcttgcaca gttacgagag gactcgcgtg
     6961 cccttccaca cggcaccttc ttcaatcgag aagaggagca ctctcacgaa tacaacaatt
     7021 tctttgccgc agatatggac agtcgcaccg ggatgcttgc tctcctggga ggaagactga
     7081 tcccgtttga ggcatactca gatgaagatg tggaccaata ttcttttgtc tctggaaaac
     7141 cttctcttct taatattgta aagaatcgta actatcgaac cgtgtattcc gcatcagaaa
     7201 ccgaaaggga actggttgtg acagacttgc catgggacca gatatacact ctaacgcatg
     7261 aagaagtgga ccaggccaga tccaaatacc tctgcatcaa tccgtacgaa ttcgaacaca
     7321 gttgcgagga caaaatcatg gtttcgcgac tggtgaacga tgtagtaaaa cacgatcgcg
     7381 tatttctctt ctacgaattc atcttcggcc atgcgtcaga gtacattgac attcagaaaa
     7441 aaacagcggt gcaatattac tctgaattca tagacgatct gatatcaggc ctgcgaaaag
     7501 cccacgagct ggaccatact ctcattgtag ttacgtcgga tcatgggatt cgcgatcacg
     7561 gctatgagtc ctggctttcc acatacagaa tccccttgct gttctatcat tcttcgttta
     7621 agaagcagct tcatccggag ctccattctc aaatcgactt tcgagatatc ttcctcgaag
     7681 aatcatcggg caaaccgtcc agtctgaagt ccagaaagtt cgcagtcttc atggggacta
     7741 caacgtcgtc tatcgtaggg gccatcacgg atcagggaga tgtgatggtc ctgaagaatc
     7801 gcaagtggct gccttacgtc atgttcaaca aaaatgcaga cgaagaagac gcgccgggcg
     7861 ggccgcccaa aagcgacctc aaacccggag cagtagtgcg atacattcag gattaccgcg
     7921 caaagttctt atcagggaac ttgccggccc actagtgtga tttggccagc tctggattca
     7981 ttctgacagt gccctgacgc tccagcttgt tccatccgta tcgcgctcct ttgagcatag
     8041 cgaacattgc cttaaaggta atgatgtaca tgaactgtct gtagaagaat ctctgaatca
     8101 acacgaggaa catcagacgc ttccgcttgt tctccagaat gaaggcaagc agtgaaccgc
     8161 agacatccat aaacaagaat agcaggtaac ccacagcgat tgcctccatg tccccccgca
     8221 gaatggagag aatgaacaca acgtcgccta tcggtgagag agccgggaaa agaacctgga
     8281 acagaaacat gttgggcaat gcaacccagc cgagcgggcc cttgaagaat gcatgcctgt
     8341 gcttccacag acactggaag gttccaaagc tccagcggaa tctttgcttg gaaagagtgc
     8401 gaatggtagc aggagcttct gtgcgcgaac gagcttccgc agcgtataca atgcgcgctc
     8461 cattctcaag catggtaaga gtgagatctg cgtcttccgt gagagtatcg ctcttgtagc
     8521 caccggcctc aagaactttg gaccgacgcc atccgcctgt agccccagga accacactga
     8581 tgcagttgag tgcgtcaaag gcacgtctgt cgaaattctg agaggtgatg tattctagag
     8641 attgaaaacc cgtcagaacg ttgtgcacgt ttccgacttc cacattcccg catacggcgt
     8701 ctacgagtgg atccttgaac ggtgctgcca gctccgtcag agtctgaggc atgacgatgg
     8761 tatccgcgtc aattgttact acgatttcgt gttttgcctc tttcattcct agatttagcg
     8821 cggaagattt gcccccgttt ggttgcgaaa ccagacgaat gcgcttgtct tttttcatca
     8881 agcctcgcac gatttctttc gtattgtccg tacttccatc atcaatcacc agcatttcga
     8941 actgagggtg cgtactttgc tgcagagatt gaatggtctg cgaaagcacc ttcgcttcgt
     9001 tatacgaagg aatgagcacg ctcacaggag gcaaaaatgt tccgtcgtcc tttctttcct
     9061 ctgctttgcg aacagatttg aagacaagaa tcccgagaaa cacaatgcga aagatcgcaa
     9121 gtaaagttgt aaaaaggaac agccatgtaa tgatcaccca tccccagcgt atgctcagcc
     9181 acgaagcggc ggtgatatat acaagcatgg cctcgcttgc aggcatcgca ggaaacaaag
     9241 tggatttgct aatccctgtt gcgtcttcca gagattggaa tacatatccc ttgcttttga
     9301 gctccggaat gccaatgcga agagcttcca cagtgcgact gcgatcgcca cctccgtcat
     9361 gcatcacaat gatatgattt cttggattct tcgcttgctc aatcagacga tctgccatta
     9421 agcgagcagt tggtttttgc cagtcggcga cgtcgatgtt tgccccgatg atcaagtatc
     9481 ccatgtccgc agccgtgcga agcggatcga tttccggggc ttcgtctgga tttgaatccg
     9541 tgttgtatgg tgcgcgaaac agcatggtct tgtagccagt ttctctttca ataatccgct
     9601 gcgtgctgtt gagctccagg cgaacgcgtt cgggagaagt cttgctgaga tcaggatgaa
     9661 agtaggtatg attgccgatc gcatgacctt ccgcgaggat ccgctgtaca atttcaggat
     9721 aacgttctgc ctgctctccc accacaaaga acgtcgcgtg cactttgctt tccttgagtg
     9781 catctagaac ttttggagtc caaactggat cgggaccatc gtcgaaagtc agaacaagcg
     9841 atttgtctgg aattgaattc ccgattcgct caatcaaata tccttccgga agggagtcat
     9901 attttgcagc gtcgataaat ccttctccat cctgctctac actcatttta ccgcgcgatg
     9961 gcacggtatg aagatgaaag acttctccaa aactcaaata gagaacactt cgaagtgctt
    10021 ctggctcgac tacagcttcg atgttgggtt tctgctgcgg caagatcttc catatggatg
    10081 gatcttccgc acccagacgc caaacaccaa ttcctgccac acccaggcga tcgatcgcaa
    10141 gcttctggtt ccaggcggtg acgttattca agaaccagac cgtgtgattg ttcccatctt
    10201 cgctgtaaga aaaatgcatg ttccgacttt ccgggtccat tccaggaagt gctcccatat
    10261 cgtctgccag agccattact tctgaaaact tcatgctgta ggagtgattt tgcgaccagt
    10321 caatcgcata ggttccaatc ccaacgatta atttctctgg tggaacgtct ttcaaagtga
    10381 acgcaacgtt ttcctcaaac cattcctgac ttgcaatcgg accagccgga ccactcgtat
    10441 agtgttcatc atatgccatc aatacaacgc cgtcggccac ctgagcgatt gcttttacat
    10501 cataagcagg atcgtgtgct gtgatgtcta ccgtgaggat catatcgcgt tcatgcaaac
    10561 gatccgccag ttcctttaga aactttacat agtccgcacg cccagcagtg ccaagagtct
    10621 cgatatcaat attcaatccc ttgagatgat cgcgctctgc gaattggacc aggacacctg
    10681 cgatgcactc tcgcaattgc gggctggcga gaagtctgcg catttccgct gtatgccatt
    10741 tctcatggtc tccgttcgtt acgcggccaa tcacggcaga atcggattcg tccaggacgc
    10801 gacgaacttc ttcatcaatt ctttcactca cttcgcattt ggcacttgtt agaaagtacc
    10861 aatcaggaaa cacaacatcg attcgatcct tgtgatcgcg aaatgccgcc acactcgcca
    10921 gatcatgctg cactaaaaac gcggtaacca gcttatctcc ctgacgggga atcgcaagaa
    10981 gtggaccttt cttatcttcc actttgcgct gcacttcgtg gccatgcttc tgttttgcac
    11041 tcagatcctg cgcctggtct cgcaaaatcc tgaccctctt gtccacattg cgtttgtgct
    11101 gaaaatcagg gagaggagga gtaacaaaga gcgcggctac aaacgcggca gctagtccac
    11161 cgcccacgag caagaatgta aagaatgcga tgagaaagga gcgccagcgc cgcgctgtgg
    11221 gatcttcaaa aatcatggat ttgccagaaa tctcaaatgg cttcgccctt cacaccctaa
    11281 ttcaaacgcg atgctggtgc gaggattgtt ctatctgacg actctggtgc cctgttcagg
    11341 ccataaaaaa agccgccctt tcgaggcggc tttcgttcag tcttagacca ctcgcttacg
    11401 gttgagcggg ctgtgctggt tgagcaggct gtgaaggctg cgtatttccg ctctggatgg
    11461 gtgctgcagg acctggagcc ttgctgcaat ctacgagagc agtataagcg tccgggttac
    11521 gtgcattgtc gcgaatcatc ttgagcagat ggtttttacg atcacaatcc gccttgtcga
    11581 agtcaagagc cgcaccatcg aaatgaactt cgaagatgtc cgtcagaacg cgaagctcat
    11641 caaagaagat gtaaacgctt tcgagagacg tatgtggctt ggaacgaagc ttgaattgct
    11701 tgaacacgag cgttttgatc gcagggaaag agttcacatc ttgagggatg ctcttaggaa
    11761 tctcaatgga cataggacgc cagccaatga aattcacaga accgaatttc agaatgtggg
    11821 tagcaccttt ccagtcttca aaccatcctt cgagatcata gtcgttacca cggcccatca
    11881 cccatacaga tactttatga acttcaccag gaagttcgat tccataaagg caatctacca
    11941 ccatcgcacg acctgcattg acagaagata gagctggatt cttgtagcag gaacgtgatt
    12001 tgtaatcacc ggtcacagca tactcgttca aaaatgggcg aggacgctct accacatatt
    12061 gatccacatg cggcgggcga atgctcacca cgttgtatcc agggaaagta aaaccaaact
    12121 tcacaccgag tactctggct tctggatagt tcacgttctc gcgaatatcc tgaggtgtac
    12181 ctttgatcag cttcacttct ctctcagctt gaagagattg agcattgggc tgatactggc
    12241 cgtctttgga agtcatatcc ttgtctgtgg acacttccca gccatagccg ccgtttgagt
    12301 aatccttgtc ccagctttcc actgtgatag atcgcagttc attggaagac acgtcactcc
    12361 caactgccgt tttgacgtct gcacttacga ttccgccgcc aagtgccaga gccactgcag
    12421 cgatgcttga cacagctgcg atggtcgccg tgcgacgatc gaagattttc ttaatcagtt
    12481 ccataggttt ctccgcacga atcatcgttt tcaccagttg tccttcactt ctgaaccagg
    12541 gtaagcattc tcgcttttat caacgagagc cttcagtcca tctacataga agtagaactg
    12601 gcccggtact tcgtgcacat cgctcgtgac aaacagactt acgaagtgca ggttcttatc
    12661 caatagcgca taacgggtgc tctgtggcag ccatccagga acggtcacag tcagttttct
    12721 ccaaccgaag aagtccagac gaccaagcgg aaggttgtga gtgtttccgc gatagtcgcg
    12781 gagcttcagg ctcatgttat gacgaaactt gcggcccagt acccagacgg agaattgacg
    12841 cgccttacct ttgattacat actcgtgagg tggtttcact tctacacgat caaagccgcg
    12901 gttgtcgaag taggtcttga ttccgaggat gtggttcggg ttgtctggat tgttttgaac
    12961 gttgagcgat tcgccttctg gtggcttgga ctgctcgtca tcggctgcgc gaatctcacc
    13021 gcgctgaacc atcttcagtg tggtggtttc tccgagcggg cacgtggagc gcgcatgcca
    13081 gtcttcagac tcttcgaagt catcgagcaa gatcacttcg agtcctcctg t
//
LOCUS       DAORFF010000021        13034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2056_length_13034_cov_3.827336, whole genome shotgun sequence.
ACCESSION   DAORFF010000021 DAORFF010000000
VERSION     DAORFF010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13034
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2056_length_13034_cov_3.827336"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PKK76_01765"
     CDS             complement(<1..557)
                     /locus_tag="PKK76_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HNL67215.1"
                     /translation="MTTQVQDRFQIDSLSLADDVVREVMKLGADACDVVVSEGADFSV
                     QVRLGRIEKLEESISRGLGIRVFKDGAVGLVHTTDFADKSIKELVKDAIAIARVSDRD
                     AANGLAPRELLGRYAGKLNIYDPSISAISPARKIEMARACEDAGLALDPRIKNSDGAS
                     WFDAEGRFTLATSDGFRGQYAGTTAG"
     gene            complement(554..1957)
                     /locus_tag="PKK76_01770"
     CDS             complement(554..1957)
                     /locus_tag="PKK76_01770"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNL67216.1"
                     /translation="MIHPRFGISVEEARRILSEGLSRGGSYADLYFEERRSSSVVLED
                     SIIKSAGRGITTGAGVRVLKGDQSGYAFSEDLTVESLIRAARTAAAIADANSSIQIAP
                     AFEGSKRNLYPASAESTLEEKIKILRDADSAARAFDPSIEKVSASISDEIQTISCITS
                     EGKVFGDVRPMVILRVSCIAQKNGKRQTGSDSAGGRFGLEYFSERRTAADIAKEAARL
                     AVLNLNAEDAPAGPMEVVLGPAESGVLLHEAVGHGLEADFNRKNLSNYSNRIGERVAS
                     EHCTIVDEGLFSNMRGSINVDDEGNEPSSAVLIEDGILRGYINDRMSAEILKTAPTGN
                     GRRESYRCLPMPRMTNTYMRPGAHDPGEILSTVKRGIYAKRFGGGQVDITKGDFVFSV
                     LESYLIEDGKITRPLKGVTLIGNGPDVMTKVVMVGNDLTFTEGGWTCGKDGQSVPVGM
                     GIPTCKVSEITVGGSRV"
     gene            complement(2009..2497)
                     /locus_tag="PKK76_01775"
     CDS             complement(2009..2497)
                     /locus_tag="PKK76_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNL67217.1"
                     /translation="MHPNEALLEKFYSAFQKRDAATMNSCYHEDVEFWDPVFHDLKGW
                     KARAMWSMLTERAAPDFKLTFSGIKADDNGGVAHWEPTYAFSKTGNLVHNIIDAKFEF
                     RDGKIIKHRDSFDLWRWAGQALGASGKFLGWTPMVQNKIRGEAATGLEMFIKRKKLGP
                     GK"
     gene            complement(2542..3039)
                     /locus_tag="PKK76_01780"
     CDS             complement(2542..3039)
                     /locus_tag="PKK76_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67218.1"
                     /translation="MKLSMTTKLIFIAIFLAAIPLSAAKPSFLADYNTTTLKVHIEDR
                     NIRVTRIEQKFDNPASAVPSSQSAAVSTGMISEAQLRSLRRLILANGFLRLKEAYGAP
                     AKERFYPYSIEVLVDGRKKKVLFRSNPSFEKQPKPFAEIEKMLTELGKEARNPEPAAT
                     DSTGK"
     gene            complement(3036..3872)
                     /locus_tag="PKK76_01785"
     CDS             complement(3036..3872)
                     /locus_tag="PKK76_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNL67219.1"
                     /translation="MQSYLIIGGNSVAGQGSVQAIRNFESNAIVHATTSTAKQADDSS
                     GIAEADKTLYGIDLSKPETLDLIPAEKYKMIIFTPAFGPIGYPIASTPESDVKEALRF
                     SYHPMVELSNRLNPDLTIGFTAFYWLPHTLSAYGSMAYAKIAQEILAVENSNHFRMIR
                     AGSFVSKATRGIGLLLQRALRDTPHAAIRDLGEKWKASGKKFADFFFDYACECEKEAF
                     GAGFDRPHRQTNQSDIERGVSMVLKGDTAPIINVLGDWTWTDDKLKPLPADFRLNVRE
                     LL"
     gene            complement(3903..4799)
                     /locus_tag="PKK76_01790"
     CDS             complement(3903..4799)
                     /locus_tag="PKK76_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNL67220.1"
                     /translation="MKFYFSDRYNVPLPANHTFPMQKYFLLRKMLLERKIVSESELIE
                     SSPVRADDLVLAHSADYVQSILLGTVSADVMRRIGFPWSEDLVVRALAAAGGTMSAAQ
                     SALEDGFSGNLAGGTHHAHRAEGEGYCIFNDFAIAIRHLIQNGKIERAAIVDLDVHQG
                     NGNASILGGRDDIYILSIQGERNFPFRRVDSTVDVDLPDGTSDFLYLETLSKFLPAVF
                     EFKPDIVFYQAGVDPLKEDRLGRLALSLEGLIQRDRMVFEQAKSAGVPVCLAIGGGYA
                     LPVELTVTAHANTFAAARSVFG"
     gene            complement(4796..5968)
                     /gene="dinB"
                     /locus_tag="PKK76_01795"
     CDS             complement(4796..5968)
                     /gene="dinB"
                     /locus_tag="PKK76_01795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNL67221.1"
                     /translation="MAARLIAHVDMDAFYAAVEQMDHPELRGKPVAVGPDPKGGKGRG
                     VITTASYEARKFGIRSAMPVSTAYRLCPHAIFVPPRFKRYAELSAIVMECLGEFSPLV
                     ETVSIDEAFLDCTGTESLFGSPSELGRKIKARIRERTGLTASAGMAGCKSVAKVASDL
                     QKPDGLTIVPEGGEKAFLAPLPIEKLWGVGARTAERLRSLGYAKVDDIARVPREEMER
                     LFGKWGAHIHDMASAIDPRPVAPGWQRKSISEEVTLEEDSSDQEFLRDMLSEIAESLS
                     QKMIGEEIRGRTLHFKIRLTGFETHTRSRTLKSYVHDMKTLRDFSWEELANFDRGGRA
                     VRLIGIGVSELLREGDEEQLDLFSARKRPDEKVDRLIFEMQEKFGGRVKRASRSKS"
     gene            complement(5977..7266)
                     /gene="purB"
                     /locus_tag="PKK76_01800"
     CDS             complement(5977..7266)
                     /gene="purB"
                     /locus_tag="PKK76_01800"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNL67222.1"
                     /translation="MIDRYSNPEISRIWELDNKYRIWLDVELAVTRALHKRGQVPDQD
                     WKEIQQKAGFDTAKILEIEEVVQHDVIAFLTNVKDHIGEAGRHLHYGMTSSDLGDTAL
                     SLQMVQAADILLKRVDELVDTARTLAIRHKNQVMIGRTHGIHGEPTTLGLKFALFYEE
                     MKRNRARLLRAREEVAVGKMSGAVGTFSANDPEIEEEVLADLGLKPDPISTQVISRDR
                     HAYFQSVLAVIAGSLDRMAQEVRLLQKTETREVEEPFRAGQKGSSAMPHKRNPVVCER
                     ICGLARVIQSNAMAGYRNMPLWHERDISHSSAERVILPDSTIALEYILGKMKFVLDGL
                     HIYPDAMQRDLELTRGLIYSQRLLLALVDSGMQREEAYTLVQSASMKVWATPGLTLRN
                     AVTEAGARLSDEKMAEVFAPEYFLRNVDFIYRRLGLS"
     gene            complement(7270..7839)
                     /locus_tag="PKK76_01805"
     CDS             complement(7270..7839)
                     /locus_tag="PKK76_01805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNL67223.1"
                     /translation="MIEIVAGTNRPGSKTIQLAQIIRGLYDLAGAPSQILDIASLPVE
                     IYQPASYKTKPAAFAPFQERILACKGIHMVVPEYNGSFPGALKYFIDMLKFPESLEHR
                     PIAYTGLADGLWGGFRAVEQLQMVCNYRNAYGLPERVWFPHIGKKMQDNRITDEFLFG
                     LLRDQVERFIKFANHHEKEAVHSIPNKSA"
     gene            complement(7897..9243)
                     /locus_tag="PKK76_01810"
     CDS             complement(7897..9243)
                     /locus_tag="PKK76_01810"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNL67224.1"
                     /translation="MAAATTALVVICSGCQTPEIEEVPFGPENPERIAQIQGQLESFL
                     AYWRARGQFYGMTVGVVKADKLVYKHETGMTQDRKYALGSITKIFTATAVMKLVEEGA
                     VDLDAPLTDYMEVRIERPELSSRPVTIRHLLSHTSGMPDLRYFHPELIASPELPFRIP
                     PQIVPAGMHYRYSNTGFMILGEMVSRRSGKPMDQFMREEIFEPLEMRQTKTSQLTGAS
                     GVATTIEDLSHFAMMYLNHGKYGGKTILSEETVNRMLTQPVYYPPAEFQEYCGLGWRV
                     KRGPKGVVTFFHIGGADYIAAWLQLFPRYRMAILYLGDPPKYDDYTMGMLLALQAKLG
                     DLATAYAGADTPLYLFKATPPPPEVFETYPGTYINVLTGREAVVTAKNDVLFFSRAGT
                     YAYPLGTQTNHVFTGGFQDLSHDFVHEPGTNKLLGIAAYDGYYVRKPPEDTDTQQN"
     gene            9338..10720
                     /locus_tag="PKK76_01815"
     CDS             9338..10720
                     /locus_tag="PKK76_01815"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNL67225.1"
                     /translation="MTFRHIRIAGFAFLGILFVVPGIRGPGFSHQVEGIPGVELIMQE
                     ASPSEWAKKRLRNIQDTLRDFVHSRAGRPALPNGLVIVNNVHEKDPIVEATQGMDRNT
                     PMTLASLTKPITATGILILVDQGKLRLDDPVSRYLPEFRMERHELGSAPITIRNLLQQ
                     TSGIPYAGRSPMVPSGVEGFFMPRQMYPAGMHHEYSNSNYELLGFIVERVTGMTFAAF
                     MQENLFGPLKMKNSRAAGVMSGASGITSTAEDLSHFAKMLLSWGRYEGMEVLSPELIR
                     EMFLPPPHIPVSENMSYYAMGWKVNVAHGRVVEAYHPGVWFNMLTDLRIYPTRKMFFV
                     TMSNPPFYRSDAATEFMGMTVGHGRRAVAYMGPSEDLEARPTQPDAGILNLYSGQYAN
                     SDGAVLRIAVQGKNIVRKMYQDERVLAPISQYEFTGDGNAMPHEFVWKNGQVAGLNTP
                     GGYYERRFPR"
     gene            10828..11268
                     /locus_tag="PKK76_01820"
     CDS             10828..11268
                     /locus_tag="PKK76_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNL67226.1"
                     /translation="MRFKNIIFPTDFSESSLHALHVADEIADKFNSQLQILHVIYDMV
                     AYADAAAVMNWYPAMYVDLEKAAKEELHKVAKKVKFAKDPQLVVRRGVEETEIIRYAD
                     EQKADLIVMGTHGRTGVGRLLLGSTAEQVLRHAHCPVLTVRSDT"
     gene            11284..11697
                     /locus_tag="PKK76_01825"
     CDS             11284..11697
                     /locus_tag="PKK76_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNL67227.1"
                     /translation="MWITYALLSAFFAALVAIFAKLGMSKVDSDLATAIRTVVILVLA
                     WSIAWFRGKLDGIVNLDRSAILFLVLSGLATGFSWLFYFRAMQMGKVSQVAPIDKLSL
                     AIVIVLSALFLGEAITWKVALGAALIIAGTIVVII"
     gene            11732..12148
                     /locus_tag="PKK76_01830"
     CDS             11732..12148
                     /locus_tag="PKK76_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67228.1"
                     /translation="MYHRDYFIRMIQQFMVLLARLAGLKAKTDPEVILMETENGLRHF
                     TGLPPELIKSLTAASLASILNARQEGDYTTLAMTALLLKAEGEARVSIKDPEGVACLQ
                     KSIDLLGYLEVDRLPIELRSSMNFKEEVEQLLQKPW"
     gene            complement(12204..>13034)
                     /locus_tag="PKK76_01835"
     CDS             complement(12204..>13034)
                     /locus_tag="PKK76_01835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL67229.1"
                     /translation="RGRVMGVVQMAFAVSQVFGIPVGLLLANQFGWHAPFLMIVGFSS
                     VVGVVIFTFMRPVRDHLHLNLGRHPMLHLWKTVTQWPYIKAFLATTLMTTGGFMLMPF
                     GAAFSVHNLGITNDQLPILYAITGVFSMIFGPIIGRLSDRVGKYPVFAAGSLLTMIMV
                     AIYTQRGITPFWFVIVLNVLLFAGISSRMISSMALMSAVPEPHDRGAFMSINSSVQQL
                     SGGVASAIAGTIVMQTRSGLIEHYDVLGYVVIVAIAITIAMMYWINLQIADKQAAHAR
                     "
BASE COUNT         3211 a         3468 c         3154 g         3201 t
ORIGIN      
        1 ccagcagtgg ttccagcgta ctgtccacga aaaccgtcgg aagtagccag agtgaatctg
       61 ccttccgcat cgaaccatga tgcaccatcc gagttcttga tcctgggatc cagtgccagg
      121 cctgcgtctt cacatgcacg cgccatttcg atttttcgcg caggtgaaat cgcgctgata
      181 gagggatcgt aaatattgag cttgcctgcg tagcgaccaa gtagttcgcg cggtgcaagt
      241 ccattggccg cgtcacgatc cgatacgcga gcgattgcaa ttgcgtcttt cacaagttct
      301 ttgatagatt tatctgcgaa atcagtagtg tggacaagtc caacggcgcc gtctttgaaa
      361 acacgaatgc ccaggccgcg cgaaatggac tcctcgagtt tttcaattct gccaagcctc
      421 acctggacac tgaaatccgc tccctcagaa acgactacat cacaggcatc tgcgccaagc
      481 ttcatgactt ctcgcaccac atcgtctgcg agagaaagcg aatctatttg aaaacgatct
      541 tgaacttgcg tcgtcatacg cgcgaaccac caacggtaat ttcagagact ttgcatgtgg
      601 gaatccccat gcccacagga acagattggc cgtcttttcc gcaagtccac ccaccttctg
      661 taaacgtaag gtcgtttcca accatgacta cctttgtcat aacatccggc ccgtttccaa
      721 tcaacgttac gcctttgaga ggacgtgtga tctttccgtc ttcaatgaga tagctttcga
      781 gaacggagaa aacaaaatcg cccttggtga tgtccacctg ccccccgcca aatctcttcg
      841 catagattcc gcgttttact gtagaaagta tttcccctgg atcgtgtgca ccgggacgca
      901 tgtacgtatt ggtcatgcga ggcatcggga ggcagcggta gctttcgcgc cgaccattcc
      961 cggttggcgc tgttttcaga atttccgcgc tcatgcgatc gttgatgtat ccgcgcagaa
     1021 ttccgtcctc aatcagcacc gcgctagaag gttcgtttcc ttcatcgtct acgttgatac
     1081 ttccgcgcat gttggaaaaa agtccttcat ccactatcgt gcagtgctca gatgcgactc
     1141 gctctccgat tcgattggag taattggaca aattctttct attaaagtct gcttccagcc
     1201 cgtgacccac cgcttcgtga agtaaaacac cgctttccgc cgggccaagc acaacttcca
     1261 ttggacctgc aggagcatct tccgcattta gattcagaac tgcaaggcgt gctgcttcct
     1321 tcgcaatgtc cgcggccgtt ctacgctcag aaaaatattc aagaccaaaa cgaccgcctg
     1381 cagaatcgct tcctgtctga cgctttccat ttttttgcgc aatgcaggac acgcgaagaa
     1441 tgaccatggg tcgcacatcg ccgaacacct ttccctctga ggtgatgcag ctaatggttt
     1501 ggatttcatc tgaaatgctg gcgctgactt tttcaatgga tggatcaaag gcacgggccg
     1561 cactatctgc atcgcgcaag attttgattt tttcttccag cgtggattcg gcggaggcag
     1621 gatacaaatt ccttttgctt ccttcaaatg caggcgcaat ttgaatcgag ctattcgcat
     1681 ctgcaatggc ggctgctgtg cgcgccgcgc gaatcaagct ctctactgtc agatcctcgc
     1741 tgaacgcata acctgattga tcgcctttga gcacgcgaac cccggcgccc gtagtgatac
     1801 cgcggcctgc agattttata atgctatctt ctagaactac ggaggaagat ctgcgctctt
     1861 cgaaatagag atccgcatac gatcccccgc gcgaaaggcc ttccgaaaga atgcgacgcg
     1921 cttcctcaac gctgatgccg aaccgtggat gaatcatttg aacaagaaaa ggccggagcg
     1981 tgtgtccggc ctccatttat cggaactgtt atttaccagg accaagcttc ttgcgtttga
     2041 taaacatttc cagaccagtg gccgcttctc cgcgaatctt gttttgaacc atgggagtcc
     2101 agcccagaaa tttgccagac gcacccagag cctgaccggc ccatctccac aaatcaaaag
     2161 aatcgcgatg cttgatgatc ttgccatccc tgaattcgaa tttcgcatct atgatgttgt
     2221 gaaccagatt gcccgtctta gagaaggcat acgtaggttc ccagtgagcg acgccaccgt
     2281 tatcatctgc tttgattcca ctgaaagtga gtttgaaatc cggagcggcc cgctctgtga
     2341 gcatggacca catggcgcgc gctttccatc ctttcagatc atgaaagaca ggatcccaga
     2401 attccacgtc ttcgtggtag caggaattca tcgtagctgc gtcccttttt tgaaacgcag
     2461 aatagaattt ttcgagaagg gcttcatttg gatgcatgac cgggcagcta gcctgccctt
     2521 ttcagatcgg gcaagcaaaa atcattttcc tgtggagtca gtggcagctg gctctggatt
     2581 tcgcgcttct ttgccaagct ctgtgagcat tttctcaatc tctgcgaaag gctttggttg
     2641 tttttcaaac gatggattgc ttctaaatag aactttcttt ttgcgtccat ctacgagcac
     2701 ttctatgcta taggggtaga acctctcctt cgcaggtgca ccataggctt ctttcaatcg
     2761 caagaaaccg ttggccagaa tcaatcggcg aagactgcgc aattgcgctt ctgaaatcat
     2821 ccccgtagat acagcagcac tctgcgaaga gggaacggca ctggcagggt tatcaaactt
     2881 ctgttcgatc cgagttacgc ggatgttgcg atcctcaatg tgaaccttca gggtggtggt
     2941 attgtagtcc gccagaaaag aaggtttggc cgcgctcagg ggaatggcag ccagaaagat
     3001 cgcgatgaaa atcaatttcg tggtcataga aagcttcata gtagttctct cacattcaga
     3061 cgaaagtctg ccggcaaagg ttttagttta tcgtctgtcc aggtccagtc tcccagcaca
     3121 ttgatgatgg gagctgtgtc tcctttcaaa accatactga ctcctctttc aatgtcagac
     3181 tgatttgtct gtctgtgcgg tctgtcaaag ccggcaccaa acgcttcctt ttcgcattcg
     3241 catgcataat caaaaaagaa atcggcgaat ttctttccag acgccttcca tttctcaccc
     3301 agatctcgaa tcgccgcgtg cggtgtatcg cgcaaggctc tttgcaagag aagtccaata
     3361 cccctcgtag ccttgctcac gaaagatccg gcgcgaatca tgcgaaaatg attcgaattt
     3421 tccactgcca gaatttcctg cgcaatcttt gcataagcca tgcttccata tgcggataaa
     3481 gtatgaggca gccagtaaaa agcggtgaac cctatagtca gatccggatt cagacgatta
     3541 gaaagttcta ccatagggtg ataggaaaat cggagcgctt cctttacgtc cgactcgggt
     3601 gttgaagcga tcggataacc aatgggcccg aaggcgggag taaagatgat catcttgtat
     3661 ttctcagctg gaatcaagtc gagcgtttct ggtttgctca aatcaatgcc gtataacgtt
     3721 ttgtctgctt ccgcaatccc ggaagaatcg tctgcctgtt ttgcagtgga tgtggttgcg
     3781 tgcacgatcg cgtttgattc gaagtttcga attgcctgca cgcttccctg cccggcaacg
     3841 ctgtttccac caatgatcag ataactttgc acggacgaaa agttccttca ggaaagcaat
     3901 cgtcaaccaa atacggaacg cgccgccgca aacgtattgg catgagccgt caccgtcagt
     3961 tcgactggaa gagcataccc accaccaatc gccagacaca cggggacacc cgcagacttt
     4021 gcctgctcaa aaaccattct atcgcgctgg atcaaaccct cgagggaaag agcgagcctg
     4081 ccaagcctgt cttctttcag cggatccaca ccggcctgat aaaagacaat gtctggctta
     4141 aactcaaaca ccgcgggcaa gaatttggaa agggtctcca gatagagaaa atcgcttgta
     4201 ccgtccggta aatccacgtc cacagtggaa tccacgcggc gaaaaggaaa atttcgttct
     4261 ccctggatac taagtatgta tatatcatct cgcccaccga gtatggacgc atttccattt
     4321 ccctggtgaa catccagatc cactatggcg gctcgctcga tttttccgtt ctgtatgaga
     4381 tgcctgatgg cgatggcaaa gtcattgaaa atgcagtagc cttccccttc cgctctgtgc
     4441 gcatgatgcg ttcctcccgc caaatttccg gaaaatccat cctccaatgc gctctgcgcc
     4501 gcgctcattg tgccaccggc cgccgcaagt gcgcgcacaa ccagatcttc gctccaggga
     4561 aaaccaattc gtctcatcac gtccgcgctt acggttccaa gcagaatgga ttgaacgtag
     4621 tccgctgaat gagccagaac cagatcgtct gcgcgcacgg gagaagattc gatcaattca
     4681 gactccgata caatctttcg ctcaagaagc atctttctca ggagaaagta tttttgcatg
     4741 ggaaacgtgt gattcgcagg caaaggaacg ttgtacctgt cgctgaaata gaacttcatg
     4801 attttgaacg actggctcgc tttacgcgac cgccaaactt ctcctgcatt tcaaaaatca
     4861 atcgatccac cttctcatcc ggcctctttc gcgctgaaaa cagatccagt tgctcctcat
     4921 caccttcgcg aagtaactcg gaaactccaa ttccgatcag acggaccgct cgcccgcctc
     4981 gatcgaagtt cgcaagctct tcccaggaaa agtcgcgcag agtcttcatg tcgtgcacgt
     5041 atgatttcaa agttctacta cgagtgtgag tttcaaaacc tgtgaggcga atcttaaaat
     5101 gcaaagtcct gccgcggatc tcctctccga tcattttctg ggacagactc tccgcgatct
     5161 cagagagcat atcgcgcaaa aattcctgat ccgagctatc ttcttcgaga gtaacttctt
     5221 cgctaatgga ttttctttgc cagcctggag ccactggcct aggatcaatg gcgcttgcca
     5281 tatcgtgtat atgtgccccc cattttccga agagtctttc catctcttcg cgcggcactc
     5341 gtgcgatatc gtctaccttc gcatatccca gagagcgcag tctctccgca gtgcgcgcac
     5401 ccacacccca taatttctcg ataggaagcg gagcaaggaa cgccttttcc ccgccttccg
     5461 gaacaatcgt tagaccatcc ggtttctgta aatcagaggc aaccttcgca acagacttac
     5521 aaccggccat cccggcactg gctgtaagac cggttcgctc gcgaatgcga gctttgatct
     5581 ttctcccgag ctcggatggg gacccaaaca aactctctgt ccccgtgcag tccagaaacg
     5641 cttcatcaat actaactgtt tctaccagag gagaaaactc gcccaggcac tccattacaa
     5701 tggcagacaa ttccgcataa cgtttgaatc gaggaggaac gaaaatcgcg tgtggacaga
     5761 ggcggtatgc ggttgagaca ggcatggcag aacgtatccc aaactttctg gcttcataac
     5821 ttgcagtggt gatgactcct ctgccttttc cacccttcgg atccgggccc accgcaaccg
     5881 gttttcctcg cagttccgga tgatccatct gctctacggc agcatagaac gcatccatat
     5941 ccacatgcgc aatgagtcga gcggccacga cctagttcag gacaggccga gtcttctgta
     6001 aatgaaatcg acgtttctca ggaaatactc tggagcaaaa acttccgcca ttttctcatc
     6061 cgaaagcctg gcccccgcct ccgtgacagc attgcgcaaa gtaagtccag gagtagccca
     6121 tactttcata ctcgcagact gcaccagagt ataggcctct tctctctgca ttcctgaatc
     6181 cacaagagca agaagaagtc tctgggaata gatgagtcca cgtgtgagct caagatcacg
     6241 ctgcatcgca tccggataaa tgtgaagtcc atccagaacg aacttcattt tgccaagaat
     6301 gtattccagt gcgatggtgc tgtcaggaag aatcactctc tcggcgctgg aatgagatat
     6361 atcgcgctca tgccacaaag gcatattgcg atatccagcc atcgcattgg attgaatgac
     6421 acgcgcgagt ccacagatcc tttcgcacac aacgggattg cgcttgtgag gcatggcact
     6481 cgaacctttt tgtcctgctc tgaaaggctc ttccacttcg cgcgtttccg tcttctggag
     6541 caacctgact tcttgcgcca tccgatcgag actccccgca atcactgcga gcacagactg
     6601 aaagtaagca tggcgatcgc gactgataac ctgagtagaa attggatctg gcttaaggcc
     6661 aagatctgcg agcacttctt cttctatttc aggatcattg gcagaaaatg ttccaactgc
     6721 tcctgacatt tttccaaccg ctacctcttc gcgcgcgcga agcaggcgtg cgcgattgcg
     6781 cttcatctct tcatagaaga gcgcaaactt caatccgagc gtggttggct ctccatgaat
     6841 tccatgggtt ctaccaatca tcacctggtt cttgtgtcga atggcgagag tcctggcagt
     6901 gtctacgagt tcatcgactc gcttgagtaa aatgtcagcg gcctgcacca tctgcaggga
     6961 caacgctgtg tctccgagat cagacgaagt cattccatag tgaagatgtc ttcctgcctc
     7021 tccaatgtga tcctttacgt ttgttagaaa tgcaatgacg tcatgctgga ctacttcttc
     7081 aatctcaagg atcttcgcag tatcaaagcc ggccttctgt tgaatttcct tccagtcttg
     7141 atccggaacc tggcctcgct tgtgaagagc tcgcgtgacc gccagctcca catccagcca
     7201 tatgcgatat ttattgtcga gttcccagat tctggaaatc tctggatttg aatagcgatc
     7261 tatcatacgt tatgcgctct tgtttggaat ggaatgtacc gcttctttct catgatggtt
     7321 tgcaaacttt atgaatctct ctacctggtc gcgaaggagg ccaaagagga attcatctgt
     7381 gatacgattg tcctgcattt tcttccctat gtgcggaaac cagactctct cgggaaggcc
     7441 gtaggcgttg cgatagttgc aaaccatttg tagctgttcc actgcgcgaa acccacccca
     7501 gagcccatcc gcaagaccgg tgtaggcaat gggtctgtgt tccaggctct ccggaaattt
     7561 cagcatatca atgaagtatt tgagagcgcc ggggaaagat ccgttgtatt ccggaaccac
     7621 catgtgaatt cctttgcatg ccagaattct ttcctggaag ggagcaaatg cggccggctt
     7681 tgttttgtag gatgcgggct ggtaaatctc aactggaagt gaggcaatat ccagaatctg
     7741 cgatggcgcg ccagcgaggt cgtatagacc gcggatgatt tgagccagct gaattgtttt
     7801 tgatccgggc cggtttgtgc ccgccacgat ctcaatcata ccgttccaga cttttcaaag
     7861 gaagcaggct gtcgcctcat ttcgaaaaca ggaaatctaa ttctgctgcg tatctgtatc
     7921 ttcggggggc ttgcgaacgt agtatccgtc ataggctgca atcccaagca acttgttggt
     7981 gccgggctcg tgtacaaaat cgtgggacag gtcctgaaat ccgcctgtaa aaacatgatt
     8041 ggtctgagtg cccagaggat aagcatacgt gcccgcgcga gagaaaaaaa gcacatcgtt
     8101 cttcgcagtc acaacagcct ctcttccagt gagcacgttg atgtatgttc cagggtaggt
     8161 ttcaaaaact tcaggcggtg gcggagttgc tttgaataaa tacagaggag tgtccgctcc
     8221 ggcatatgcg gttgcaagat cgcctaactt tgcctgaagt gccagaagca ttcccattgt
     8281 gtagtcgtcg tactttggag gatcacccag atacagaatc gccatgcgat atcttggaaa
     8341 caattgaagc caggcggcaa tgtagtctgc gccaccaata tggaagaagg taacaactcc
     8401 ctttggccct ctcttcactc tccacccgag cccgcaatat tcctgaaatt cagcaggagg
     8461 ataataaacc ggctgcgtga gcattcgatt cactgtctct tcagataata tagttttgcc
     8521 tccgtatttc ccgtgattca aatacatcat ggcgaaatgc gataaatctt caatcgtcgt
     8581 ggccactcca gacgctccgg taagctgcga tgtcttggtc tggcgcatct cgagtggctc
     8641 aaagatctct tcgcgcatga attgatccat gggctttccg cttctgcgcg aaaccatttc
     8701 cccgaggatc ataaagccgg tgttagaata tcgatagtgc atccccgcag gaacgatctg
     8761 cggcggaatg cgaaagggca gttccggaga tgcaatcaac tcaggatgaa agtagcgaag
     8821 gtctggcatc ccagaagtgt gcgaaagcaa atgccgaatc gtcactggcc tggaggaaag
     8881 ttcgggcctc tcaatgcgaa cttccatata atcggtgagc ggcgcatcga ggtccaccgc
     8941 cccctcctcc acgagcttca tgactgcggt ggcagtgaag atcttcgtga tcgacccgag
     9001 cgcgtatttt ctgtcctggg tcatgccggt ctcgtgcttg tagacaagtt tgtctgcctt
     9061 gaccaccccg accgtcatcc catagaactg tccgcgggcc ctccagtagg ccagaaagct
     9121 ctccagctga ccctggattt gggcgattct ctcaggattt tctgggccaa atggcacttc
     9181 ctcgatttct ggcgtctggc agccagaaca aatgacgacc agagcagtcg tggctgccgc
     9241 caaaaggtgc ttgaaaactg gggcaatttg aaaaaaatgc cctctcttcg agggaaaagc
     9301 gttcatccga taataaaatg gggcaaatga ggttctaatg acatttcggc atatacgcat
     9361 cgcaggtttc gccttcctgg gtattctttt tgtagtacca ggaatccgcg gaccaggctt
     9421 ctcccaccag gtggaaggaa ttccgggtgt ggagctcatc atgcaagagg ctagcccctc
     9481 tgaatgggcc aaaaaacgtc tccgcaacat tcaggacact ctgcgcgatt ttgttcacag
     9541 ccgcgcaggc agacccgcac tccccaatgg actggtcatt gtaaacaatg tccacgaaaa
     9601 agatccaatc gtagaagcaa cccagggaat ggacagaaac actcccatga cgcttgcttc
     9661 tttgacaaag ccaatcactg caacaggtat tctgattctt gtagatcaag gcaaactccg
     9721 cctggacgat ccagtgtctc gctatttgcc agaattccgg atggaaagac atgaacttgg
     9781 cagcgctccg atcacgattc gcaatctact tcagcaaaca tccggcattc cgtatgctgg
     9841 cagatcgccc atggttcctt ctggagtgga aggtttcttc atgccgcgcc agatgtatcc
     9901 tgcaggcatg catcatgagt attccaactc caactacgaa ctgctgggct tcattgtaga
     9961 gagagtcaca ggaatgactt tcgccgcgtt catgcaggaa aatctgtttg ggccactgaa
    10021 aatgaaaaac tcaagggctg cgggagtgat gtcaggcgca tccggaatca caagcaccgc
    10081 agaagatctt tcgcacttcg cgaagatgct tctgagctgg ggaagatacg aagggatgga
    10141 agtgctttct cctgaattga ttcgtgagat gtttcttcct ccaccgcaca ttccagtttc
    10201 agaaaacatg tcctactacg cgatgggttg gaaagtaaac gtggcccacg ggcgagtcgt
    10261 agaagcgtat catcctggag tatggttcaa catgcttacg gaccttcgca tctatccaac
    10321 gcgaaaaatg tttttcgtaa ctatgtccaa tccgcccttc tatcgctcgg acgcggccac
    10381 tgaattcatg ggaatgaccg taggacacgg ccggcgtgcc gtcgcttata tggggccatc
    10441 ggaagatcta gaggcaagac ctactcagcc agatgcgggc attctgaatt tgtacagcgg
    10501 gcagtatgca aatagcgacg gagcagtact tcgcatcgca gtgcagggca agaatattgt
    10561 acgcaaaatg tatcaggatg agcgagtgct tgctcccatt tcacagtacg aattcacagg
    10621 agacggcaat gccatgcctc atgaattcgt ctggaaaaat ggtcaggtag ccgggctcaa
    10681 tacacctggc ggctactacg aacgccgttt tccgcgctaa tttccacact tccggccatc
    10741 ctgacaaaag tcatagggct tcagtgacag caaatctacc ttgcccgatc cgggcatggt
    10801 agactaagct tacatttgga ggccaccatg cgctttaaga atatcatctt tccaacggac
    10861 ttttcagaaa gctccctgca cgctctccac gtcgcagacg aaatcgcaga caagttcaac
    10921 tcacagctgc agatccttca cgtgatctat gatatggtgg cctatgcaga cgccgcggcg
    10981 gtgatgaact ggtatccggc catgtatgtg gatctagaaa aagcggcaaa agaagaactg
    11041 cacaaagttg cgaagaaagt aaaattcgca aaagatcctc aactggttgt gcgacgcggc
    11101 gtagaagaaa cagaaatcat tcgatacgca gacgaacaaa aagcggatct cattgtgatg
    11161 ggaactcatg gacgcacagg tgtgggcaga ttgcttcttg gaagcaccgc agaacaggta
    11221 ctgagacatg cccattgccc cgtgttgact gttcgttctg acacttgatc cacggccaaa
    11281 cttatgtgga tcacctacgc gcttctctcc gccttctttg cggcgctcgt tgccattttt
    11341 gcaaaacttg ggatgagcaa ggtggacagc gatctggcca cagccattcg cacagtggtg
    11401 atcctggtgc tggcctggag catcgcatgg tttcgcggga agttagacgg cattgtgaat
    11461 ctggatcgaa gtgccatcct gtttctggtg ctttctggcc tggccaccgg attctcctgg
    11521 ctcttttact ttcgcgccat gcaaatggga aaagtttctc aggtggcgcc cattgataaa
    11581 ctcagcctcg cgatcgtgat tgttctttcc gcactcttcc ttggagaagc aatcacctgg
    11641 aaggtggctc tcggggccgc tttgattatt gcagggacca ttgtggtgat catctaacgt
    11701 tagtcggcca tgccgctgga gaatccggag tatgtatcac cgcgactact tcatccgcat
    11761 gatccagcaa ttcatggtgc tcctggcgcg tctggctgga ctcaaagcaa aaacagatcc
    11821 ggaagtgatt ctcatggaaa cggaaaatgg cctgagacat ttcacggggc tgccgcctga
    11881 gctcatcaag agcctgactg ctgcttcact cgcatcgatt ctaaatgcca ggcaggaagg
    11941 ggattacacc acacttgcca tgactgctct tcttctgaaa gcagaggggg aagcgcgtgt
    12001 atcgatcaaa gatccggaag gagttgcgtg tctgcaaaaa tcaatcgatc tcctggggta
    12061 tctcgaggta gatcggcttc cgattgaatt gcgttcttcc atgaacttta aagaagaagt
    12121 ggagcagcta ttgcaaaagc cctggtgaac aactgcggcg gcagtgagcg cctccctgga
    12181 aggcgcgtca gaccgctgtt cgcctaacgc gcgtgcgccg cttgtttatc tgcaatttgt
    12241 agattgatcc agtacatcat cgcaatcgta atcgcaatgg caactatgac cacataaccc
    12301 agcacatcat agtgctcaat gagtccgctt ctggtttgca tcacaatagt tccggcaatc
    12361 gcagaggcca cccctccgga aagctgctgc acggatgaat tgatgctcat gaacgcacct
    12421 ctatcatgtg gttcaggaac agcggacatg agagccatag acgaaatcat gcgcgaagaa
    12481 atccctgcga aaagaagaac attcagcacg atgacaaacc agaacggagt gatacctctt
    12541 tgagtataga tggctaccat gatcatggtc aaaagcgatc ctgccgcaaa tacgggatat
    12601 tttcccacgc gatcgctgag tcttccgatg attggaccga agatcatgga aaacactccg
    12661 gtgatcgcgt acagaatggg gagctggtca tttgtaattc ccaggttgtg aacagagaat
    12721 gcagcaccaa atggcatgag catgaagcca ccggtcgtca tcaatgtggt agcgaggaat
    12781 gctttgatgt atggccactg agttacagtc ttccagaggt gaagcatggg atgcctgccc
    12841 aggttcagat gcaggtgatc cctcacgggt cgcatgaatg tgaaaattac tacacccaca
    12901 acagaactaa aaccaacgat catcagaaaa ggagcgtgcc atccaaactg attggcgaga
    12961 agtaatccga ctggaatacc gaacacctga ctgacggcga aagccatctg aactacgccc
    13021 atcactcttc cgcg
//
LOCUS       DAORFF010000022        12776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2128_length_12776_cov_3.422058, whole genome shotgun sequence.
ACCESSION   DAORFF010000022 DAORFF010000000
VERSION     DAORFF010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12776
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2128_length_12776_cov_3.422058"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PKK76_01840"
     CDS             <1..683
                     /locus_tag="PKK76_01840"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNL67230.1"
                     /translation="TTAASNGSVNAMHDSMMPLTGAVELFNMHLGEIVFGGVGSGMYG
                     MLFYVILTVFLAGLMVGRTPEFSNKKIEKFEVQMAGLAILGPGVAILFFTATALLVKA
                     GTATLNNSGPHGFSEVLYAFTSASANNGSAFAGLGVNNDFYNYALGACMLIGRFVCLI
                     PAILIGGSLALKKSVPESTGSFRTDTPVFVILLVFFILLIGGLTFLPALTMGPIVEHF
                     VLKAGSLF"
     gene            697..2763
                     /gene="kdpB"
                     /locus_tag="PKK76_01845"
     CDS             697..2763
                     /gene="kdpB"
                     /locus_tag="PKK76_01845"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HNL67231.1"
                     /translation="MKKFSVFSLDKNIVGAAFIAALRKLDPRAQIRNPVMFMVYVGAI
                     MTSIPAFWQGSAASLSFKIQLVSWLWLTILFANFAGALAEGQTKARTDALRTTRGSVL
                     AKKTSSIEEKKFSVVNGQLLRKGDLFVCEAGDDIAADGEIIAGAATVDESAITGESAP
                     VIRESGGDRSAVTAGTRVLSDRIVIRVTQNPGDSFLDRMITLIEGAKRSKTPAEIALT
                     IVLSVFSIVFLVAVMSLPVFYEFAAGGKSSIPLPSLIALLVCLIPTTIAGLLSAVRIS
                     GMNQLLAHNVLAMSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFFPAPGVEEYDLAS
                     WAQLASLADETPEGRSIVVLAKNKFSLRERSLSDISAIAVPFSATTRMSGSDLNMDGK
                     QVAVRKGAFDAIAARVQSMNGSIPDAVKTRVDEIAKKGGTPLVVARDATVMGCIYLKD
                     IVKGGIRERFVELRKIGIRTVMITGDNALTAASIAAEAGVDDYIAEAKPETKLARIRT
                     EQSRGHMVAMIGDGTNDAPALAQADIGVAMQTGTQVAREAGNMVDLDSNPTKLLEIVE
                     IGKKILMTREALTTFSIANDVAKYFAILPAILGSLYAVAGVSPIASLNVMRLSSPESA
                     ILSAVIFNALIIIAMIPIAMKGVQFRPMNASALLSRNLLIYGLGGLIAPFIGIFALDI
                     IVRRIV"
     gene            2760..3341
                     /gene="kdpC"
                     /locus_tag="PKK76_01850"
     CDS             2760..3341
                     /gene="kdpC"
                     /locus_tag="PKK76_01850"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HNL67232.1"
                     /translation="MRIIKHAFLSLASLIVLTVFTGWFYPWLVTTIARSAFPDRAEGS
                     LVRIGGEIRGSELIAQKFSGDRYFWPRPSAGDYATVPSSASNLGPSSAALAKAIQGRK
                     DSLAKAHNGIPPADMVTTSASGLDPEISPEAARYQLDRVARARRLNESQKEELRTLVE
                     QSVLEPTFGFLGESRVNVLRLNLALDERYPIQQ"
     gene            3338..5968
                     /locus_tag="PKK76_01855"
     CDS             3338..5968
                     /locus_tag="PKK76_01855"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HNL67233.1"
                     /translation="MTGEDFRATPEALLELARKEETTKQRGKLKIFFGMAAGVGKTYA
                     MLSEARQLLDDDADVVIGILETHGRIETIARAEGIPFIPRKEIQSTREDGESFTQHEM
                     DLDSILRRKPAIVLVDELAHTNAPGSYHPKRYMDVEDLLEGGIHVYTTLNVQHLESVA
                     DFVEQITGAAVQERVPDRILDLANEIELIDISPDELRKRLHEGKVYRSDRAEVAARNF
                     FKPENLLALREMALQTTARVVDTESLKRRTSGEVLRTRERILVLVGTDRFAARLIRIG
                     RRMAADLKAPWFVMHVRSSHSRGEDNSRIESEFNLARSLGAEVLLMDGDLVDAVEQVI
                     AERSITQMLVGRTEHRFSFSRPITHRLIDRVRNADIHIVPIKREINARKRRSYRLPEL
                     RSPFKEYAVASGVIWTLTVGCLVAFPDGQYSTPSIIFIAAIAALGLVFGRGPVLSAAV
                     QSAFAWDFLFIPPRYTLTIGKLEDRLMFGLFFAVAIVTGILTSRLRRSKADLLRRQRD
                     LVQIQEISQALAGATGVEEITGLVCNKLSAIFDIPDCMILLKQGKISTMLPATASIDA
                     KEQAVADWVVENGKPAGKGTDTLPLSAALWIPLKTPSRGTMGALRLPVENPDINELNL
                     VGAIAAQASLALERELLASSARQAAVNEESEKLFHAILRSISHELRSPVTAMLAAAEN
                     LSRDRVMMDDLLRNEVRQEISDSSQRLARVVDQLLAMSRIESGRLTLQLENHDLRDLL
                     QECMAQLGKEFAGHTVKIDASEKVEARFDFPMMRQALIGVLHNAALHTPIHTIVQIWI
                     FRERASAVIIIEDNGPGIPGDPGKLFEPFFRGAAAKPGGLGLGLSIAKSVVELHGGSI
                     VATRTDTGSRFRIELPLEKS"
     gene            5965..6639
                     /locus_tag="PKK76_01860"
     CDS             5965..6639
                     /locus_tag="PKK76_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNL67234.1"
                     /translation="MKEKILLIEDEKPIRKMLSINLESEGYTVLEADSGKEGVQVCAR
                     NLPDLVLLDLMLPDMQGQEVLRMLREFTVVPVIVLSAVSSEKSKIELLDRGADDYLTK
                     PFSPGELLARVRVGLRHAQSSKTESVLEAGPIRLDPSDRTVQIRGNQIHLTPTEFAIL
                     ALLLQYAGKTVTREILIQTIWGSNENETGSLRVHINQLRKKIETSAAMPELILNEPGV
                     GYRLAV"
     gene            complement(6660..7235)
                     /locus_tag="PKK76_01865"
     CDS             complement(6660..7235)
                     /locus_tag="PKK76_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67235.1"
                     /translation="MHSEFYQIGIVVAILIAGYFYWRNEKLKEKIKDLSGTSGGVPST
                     VSQFRSQQNAEEPQLSIPEDESAKPEANPEEEKKEYNYRDPKFGMPPIASTKRILGRE
                     ENKFGDAVYGRDSGYDSNAYIGRVSRGAELELLDRRIYKNEFEIDIIKVKVLSNEWAT
                     EVGQTVWLGLNDTSFRSMVSQDGSEIQEPRE"
     gene            complement(7270..7758)
                     /locus_tag="PKK76_01870"
     CDS             complement(7270..7758)
                     /locus_tag="PKK76_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HNL67236.1"
                     /translation="MSEPIQLIREEMFDSLAAEARAKPRLRINHNFHDSMDDNPHRFL
                     NVLLKGTYLCPHRHQFPPKAESFLILQGSIAFFIFDDSGVVQQIHTLSADGPVRGIDI
                     APGIWHGLLVLSERCVCFEVKPGPYRPSDDKEFASWAPREGDPRVGEMLARLEREYAL
                     RK"
     gene            7757..8797
                     /locus_tag="PKK76_01875"
     CDS             7757..8797
                     /locus_tag="PKK76_01875"
                     /inference="COORDINATES: protein motif:HMM:NF016932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HNL67237.1"
                     /translation="MSEVLKSEENPYRTQSASLESDGRTVYLYLNPVEGVDLVPRAVW
                     VMNLVPAPAEDADGMIRGQAPVRSRAACAHPNGMKAPHENEIDFVWFQEGTGVTLCVE
                     GEIWAVIPPFSGSDGFYGFCREATLPEHGTFPLPDGMKARAKENLDFWNRRTQKGFWA
                     QFQGNLLRTYESAFGPHNNYYVLDNGKFPAMGLAEFIIPDTTLRLFATIGMSAQNMPG
                     VELHVKNPEPLFRVELLTVRDAPHPWFIDSMSRVALLPWRTGGWVGPGHSIQFGRSEP
                     MSDYLVLEHHLVQDKILADLFAKLPSVFLDERYARTFLFLWPAVPDDFRVNQARGREF
                     LAKKNSVVERFS"
     gene            8794..9837
                     /gene="rsgA"
                     /locus_tag="PKK76_01880"
     CDS             8794..9837
                     /gene="rsgA"
                     /locus_tag="PKK76_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNL67238.1"
                     /translation="MISLDPRFEEDFKKIRDSGFEPGRVVSQTVTYEVATESGDYTAQ
                     PAGKLLFSGMLPAVGDWVALQVYEGGAIIHDIVPRKTVIMRKSVGKSSADQVIAANLD
                     YAVVVMGLDSNFNLRRLERYTTIIAASGAKGIIVLSKADLADPEEIKLKVDHTIACAP
                     GLPVIVWSDADKRGLEELMAILRGPATFCLLGSSGAGKSTLINAIAGKPIARTGAVRT
                     SDEKGKHTTTVRTLISLGNGIDLIDNPGMREIGLTSEEGLSDAFPEITQAAAECKFQD
                     CTHENEPGCAVELAVQEGRITAERFASYMRLLHESRTIESMKSERGRQKKKQNEKTLA
                     KMIRARLKDKGRS"
     gene            complement(9815..11668)
                     /gene="recD"
                     /locus_tag="PKK76_01885"
     CDS             complement(9815..11668)
                     /gene="recD"
                     /locus_tag="PKK76_01885"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HNL67239.1"
                     /translation="MSRTVREAARENPELYLWVSLVDDLLEFSHFPGDESIRALLLLL
                     ADTCMHGDLCMRLDDLSLTSTRDRFVQRHDTTEPNLEDAIVAAKSINRYSPLIGQDEK
                     SPVVLSDTFLFFQKYFLLRRNIERDFARMAAQPPRPLEKAKEAYGTALSVSGLTLSAE
                     QKLAVALSLFSSFVIITGGPGTGKTTVIRAVLDSFLRAGIPADRVILCAPTGRAARRM
                     AETLGDQYTASTIHTALSYRRDSQDFYFGPNRKLIGDVLVVDEVSMVDVDLMSRLLAA
                     VDPTRTRVILIGDRNQLPSVDAGAVLGDLVELFALSGNPQYSPDFASWLRTLDPELDL
                     PQMPGKSRAVDRMVHLTASHRSADVVIRASDAVRKAEESLPWSIVPLRELPENHGVVQ
                     IAFRDGGQNEVLGAWLKLSYGPEFVDLLREASALDADDPAAVDVMRHLTEVTGRARIL
                     SALRQSEDGSVRLNRRMMKLFLKQNRSVMFGEWFSGLPLMITRNDYSRELFNGETGLL
                     VRFRTGFRWIAARGEEIRLLPPESLPECEPAFVTTVHKSQGSEYNRVLLWLPESDHPM
                     VSRQILYTALTRAKELVWIYGKESVLRAGIQRNPRRETGGLSLDSMNDPYP"
     gene            complement(11665..>12776)
                     /locus_tag="PKK76_01890"
     CDS             complement(11665..>12776)
                     /locus_tag="PKK76_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNL67240.1"
                     /translation="IQRFEEYKRLYYVAFTRARDRVFAPFFPSGRGGPLSHLVAPAAD
                     QVLAQNLARLVQPMHAGPPEPLTRTAKAPGRTELSRFPTNLSVRAAGLTSYSGILRRA
                     VALDALRAEFGDTNPAEDEPLPLPETRDPTLDQKGNFLIVDHPELPRGEATGNAIHEA
                     LEGLDFSEFDRKQVPKPGTLEPATRDHIQACLNEYGLVGEDMFRAALAMIHSALNADL
                     KGIGRLCKVKHKVPEFRFLARAQGFSGDKNLLVRDGYIHGAIDLVFRVKDTYYILDWK
                     TNTLPDYSESSLQTSIAEERYDLQYSIYGHALISWLNSSGKFEPSRFGGVFYLYLRGM
                     KPGETDGVYFVRPDPAALEDLAGIIRRFSAGEPLV"
BASE COUNT         3201 a         3331 c         3297 g         2947 t
ORIGIN      
        1 cgaccacagc tgcgtccaac ggctccgtaa acgcgatgca cgacagcatg atgcctctta
       61 caggtgctgt ggaattgttt aacatgcatc taggagaaat cgtgttcggc ggtgtcgggt
      121 ccggaatgta cggaatgcta ttctacgtaa tacttacggt attcctcgct ggtctgatgg
      181 ttggccgcac gccggagttc tcaaacaaga agatcgagaa attcgaagtg cagatggctg
      241 gtctggcaat tcttggaccg ggcgttgcca tcttattttt tactgcaaca gcactgcttg
      301 tcaaagcagg aacagcgaca ttgaacaact ccgggccaca tggcttttca gaagtgctct
      361 acgcatttac ttccgcatcc gcaaacaacg gcagcgcatt cgcaggactg ggagtaaaca
      421 atgatttcta taactatgcc cttggcgcgt gcatgctcat tggccgcttt gtttgtctga
      481 ttcccgccat tctcattggt ggttctcttg cgcttaagaa atccgttccg gaatccactg
      541 gatcgtttcg cacagacact cctgtattcg tgattctgtt agtgttcttt atcctgctca
      601 tcggtggtct cacctttttg cccgcgctta ctatgggacc aattgtagag cacttcgtcc
      661 tgaaagcagg ctcacttttt tagctggagc aatgatatga aaaaattcag cgtattctca
      721 ctcgacaaga atatagtcgg cgcggcattc atcgcagcgc ttcgcaagct agatccgcgc
      781 gctcaaatac gaaatccagt catgttcatg gtgtatgttg gcgccatcat gacaagcatc
      841 cctgccttct ggcaaggaag cgctgcttct ctcagcttta agattcagct ggtttcctgg
      901 ctgtggctga ccatattatt tgcgaatttc gcaggggctc tggcggaagg acagacgaaa
      961 gctagaacgg acgctcttcg cacaacccgt ggaagcgtgc tcgcaaaaaa gacctcgtcc
     1021 attgaggaga agaagttctc tgtcgtgaac ggacagctcc ttcgcaaggg cgatctgttt
     1081 gtttgcgaag ccggagatga cattgccgct gacggagaaa tcatcgcagg cgcggccaca
     1141 gtggatgaat ctgcgatcac aggagaatcc gcccctgtca ttcgagaaag cggaggagac
     1201 agatctgcgg taaccgcggg gacgcgtgtg ctcagtgatc gcattgtgat tcgtgtgact
     1261 caaaatccag gtgattcgtt tctcgatcgc atgatcacgt tgatagaagg cgcaaaacgt
     1321 tccaaaactc cggcggaaat cgcacttacg attgtactgt ccgtcttctc catcgttttc
     1381 ctggtagccg tgatgtctct gcctgtattc tatgaattcg cagcaggtgg caagtccagc
     1441 attccactgc cctctctcat cgctcttctt gtctgcctga ttccaaccac aatcgcaggg
     1501 ttgctctccg cagtgcgaat cagtgggatg aatcaacttc tagcgcataa cgtcctcgcc
     1561 atgagtggtc gtgcggtaga agcggcgggg gacgtagacg tgctactgtt ggacaaaacg
     1621 ggaaccatca cactgggcaa tcgccaggca tccgagtttt ttccagcacc aggcgtagaa
     1681 gagtacgatc ttgcatcctg ggcgcaactc gcatctctcg cggatgaaac accggaaggc
     1741 aggtcgattg tcgttctcgc caagaataag ttctctctgc gcgaacgatc tctttcggat
     1801 atttccgcaa tcgcggttcc tttttccgca acgactcgca tgagcggcag cgacctgaat
     1861 atggatggca agcaagtcgc tgtgcgcaaa ggcgcatttg atgccatcgc cgcacgagtc
     1921 cagagcatga atggaagcat tccggatgcg gtaaaaactc gtgtagacga aatcgcaaag
     1981 aaaggaggca cacctctagt ggtggcgcgc gatgccactg taatgggctg tatctacttg
     2041 aaagatattg tgaagggagg aatccgcgaa cgatttgttg agctgcgaaa aattggaatt
     2101 cgcactgtga tgatcacggg ggacaacgcg ctgactgcag cttccatcgc tgcagaagcg
     2161 ggcgtagacg actacattgc agaagccaaa ccggaaacaa aactggcgcg gatccgaacg
     2221 gaacaatcca gagggcatat ggttgccatg atcggagatg gaacgaatga cgcacctgca
     2281 ctcgcacagg cagacatcgg agtggccatg caaaccggaa cgcaggtagc acgggaagct
     2341 ggcaatatgg tagatctgga ctccaatcca accaagctcc ttgagatcgt agaaattgga
     2401 aaaaagatac tcatgactcg tgaggcgctc actacgttca gcattgcaaa cgatgtcgca
     2461 aaatactttg cgattctgcc cgcgatttta ggatcgctct acgcagtcgc aggagtctcc
     2521 ccgattgctt ctttgaacgt tatgcgactt tcttcacctg agagtgcaat cctcagtgca
     2581 gtgatcttca acgccttgat catcattgca atgattccta tcgccatgaa aggtgtgcaa
     2641 ttcaggccaa tgaatgcaag tgcacttttg tccagaaacc tgttgatcta cgggctcggc
     2701 ggtctcatcg ctcctttcat aggaatcttc gcactggaca tcattgtaag gaggattgta
     2761 tgagaatcat caaacatgca tttttaagtc tggcttctct gatcgtgctc acagttttca
     2821 cgggatggtt ctatccctgg cttgtaacaa caatcgccag atctgcgttt ccggatcgag
     2881 cggaaggcag cctggtgcgg attggcggtg agattcgcgg atccgaactg attgctcaga
     2941 aattctcagg tgatcgctat ttctggccca ggccatccgc cggagactac gcgacagtac
     3001 catcctctgc atcgaacctt ggcccttcga gcgctgccct cgcaaaggca attcaaggaa
     3061 gaaaagactc actcgcaaag gcgcataacg gcattcctcc ggcagacatg gtcacaactt
     3121 ccgcaagcgg cctcgatccc gaaatctcgc ctgaggcagc gcggtatcag ctggacagag
     3181 tagcaagagc cagacggctc aacgaaagcc agaaagaaga gctgcggacc ctcgtggagc
     3241 aatcggttct tgaacccaca tttgggtttc tcggggaatc cagggtgaat gtattgaggt
     3301 tgaacctggc gctggacgag cgatacccga ttcagcaatg acaggcgagg acttcagagc
     3361 aacgcccgaa gctcttctcg agctcgcgcg aaaggaagag acgaccaaac aacgcggcaa
     3421 gctgaagatc ttttttggca tggccgccgg agtgggtaag acgtatgcaa tgctctcgga
     3481 agcccggcaa cttctagacg acgacgcaga cgtagtcatc ggaatcctcg aaacccacgg
     3541 aaggatcgag acgattgcga gagcagaagg aattccgttc attcctcgaa aagaaatcca
     3601 gagcacgcgc gaagacggcg aatctttcac tcagcacgaa atggatctgg actcgatttt
     3661 gagacgaaag cccgcgattg ttctcgtcga tgaacttgca catacgaatg cacctggctc
     3721 ttatcacccc aagcgataca tggatgtgga agatttgctc gagggcggaa ttcatgtcta
     3781 cacgacgctc aatgtccaac acctggaaag tgtagccgat tttgtggaac agatcactgg
     3841 agccgccgtg caggagagag tgcccgatcg cattctggat ctggcaaacg aaattgaact
     3901 gattgatata agtcctgacg aacttcgaaa acggctccac gaaggcaaag tttacagaag
     3961 tgatcgcgcg gaagttgccg cgcgaaattt ctttaaaccg gagaatctgc tcgctttgcg
     4021 cgagatggcg ttgcagacca ccgcgcgcgt agtggataca gaatcgctca agcgaagaac
     4081 atccggagaa gtgctacgaa cgcgcgaacg aattctggtc ctggttggca cggatcgctt
     4141 cgcggcgcgt ctgattcgaa ttggacgcag aatggccgca gatttgaagg cgccgtggtt
     4201 cgtaatgcat gttcgctcct ctcatagtcg gggagaagac aattccagga tcgaatcgga
     4261 attcaatcta gcgcgctcgc tgggcgcgga agtacttctc atggatggag atctggtaga
     4321 cgccgtggaa caggtgatcg ccgaacgcag cattacgcaa atgctcgtgg gaagaacaga
     4381 acatcgcttt tccttttcgc gacccatcac gcatagactg attgatcgcg ttcgcaatgc
     4441 agacattcac attgttccga taaaaagaga aatcaacgct cgcaaaagaa gatcctacag
     4501 actccctgaa ctccgctctc ccttcaagga atacgcagtt gcaagtgggg tcatatggac
     4561 tttgacagtc ggatgcctgg ttgccttccc tgatggacag tattccactc catcgatcat
     4621 ctttatagcg gccatcgcag ccctggggct ggtgtttggt cgtggacccg tgctgtcagc
     4681 cgccgttcag tctgcgttcg cctgggattt tctctttatt cctccccgtt atacgctcac
     4741 gatcggaaaa ctcgaagatc gactcatgtt cggattgttc ttcgcagttg ccattgtgac
     4801 gggcattcta accagtcgcc ttcgcaggag taaggcggac ctgctgcgaa gacagaggga
     4861 cctggtgcaa atccaggaaa tatcgcaggc actcgcaggc gcaaccggcg ttgaggaaat
     4921 cacgggtctt gtttgcaata aactgagcgc catttttgat atcccggact gcatgatatt
     4981 gctcaaacaa ggcaagatat ctaccatgtt gcctgcgact gcaagcatcg atgccaaaga
     5041 gcaggcagtc gcggactggg tagtggagaa tggcaaacca gctggaaaag gcacggatac
     5101 tttaccgctt tccgccgcac tctggattcc tctgaagact ccttcgagag gcaccatggg
     5161 agcacttcgt ctgcctgtgg aaaatccgga catcaatgag ctgaatcttg tcggtgcaat
     5221 agcagcacag gcgtctcttg cgctagaacg cgaactcctg gcctcaagcg ctcgccaggc
     5281 ggcggtcaat gaagaatccg agaaattgtt tcatgccatc ctgagatcga tttcgcatga
     5341 acttcgttct ccagtgactg ctatgcttgc tgcagcggaa aatctctcga gagaccgggt
     5401 catgatggac gatctcttgc gaaatgaagt ccgtcaggaa atttcagatt cttcacagag
     5461 gcttgcccgc gtggtggatc agttgctcgc gatgagcaga atcgagagtg gtcgcctgac
     5521 gttgcaattg gaaaaccacg atctgcgtga cctccttcaa gaatgcatgg ctcaattggg
     5581 caaagagttc gcaggtcaca cagtcaaaat cgatgcgagc gaaaaggtgg aggccaggtt
     5641 tgattttccg atgatgcgac aggctttgat tggagtactc cacaatgcgg ctctccacac
     5701 accgatccac acaatcgtcc agatctggat tttcagagaa cgagcatctg cagtgatcat
     5761 catagaagac aatggacctg gaatcccagg agatccagga aagctatttg agccattttt
     5821 ccgaggtgcg gcggctaagc ccggtggact cggtcttggc ctttccattg cgaaatcagt
     5881 ggtcgagcta catggaggaa gcattgtagc aaccagaaca gacacgggga gtcgctttcg
     5941 catcgaactg ccgctggaaa aatcatgaaa gagaaaattc ttttgatcga agacgaaaag
     6001 ccaatccgca aaatgctttc gatcaatctt gaatcagaag ggtatacagt actcgaagcg
     6061 gactcaggaa aggaaggtgt gcaggtttgc gcaagaaatc tacctgatct tgtgttgctc
     6121 gatctgatgc ttccggacat gcaagggcag gaagttcttc gcatgttaag agaattcact
     6181 gttgtccctg tgatcgtact ttctgcagtc tcatctgaaa aaagcaagat cgaactactc
     6241 gatcggggcg ctgacgatta tctcacaaaa cctttcagcc cgggagaact cctggctcgc
     6301 gtccgcgttg ggctaagaca tgcgcagtcc agcaagacag aatctgtcct ggaagctggt
     6361 ccaattcgtc tcgatccgtc tgatcgcacc gttcagatac gcggaaacca gattcatctt
     6421 actccaacgg aatttgcaat cctggcactt ctgcttcaat acgctggaaa aacggtgacg
     6481 cgtgaaatcc taatccaaac aatctgggga agcaacgaaa acgaaacagg aagtctgcgc
     6541 gttcatatca accagcttcg caagaagatc gaaaccagtg cagccatgcc cgagctaatt
     6601 ctaaacgaac caggagttgg ttacagactt gccgtgtaac gttattctca atgacccatt
     6661 tattcgcgcg gctcctggat ctcactgccg tcctgcgaaa ccatagagcg aaaacttgtg
     6721 tcatttaaac caagccagac ggtctggcca acctccgttg cccactcgtt ggaaagcact
     6781 ttcactttga tgatgtctat ttcgaattca ttcttataaa ttcttcgatc cagaagctca
     6841 agctccgccc cgcgagaaac acgaccaatg tacgcattcg aatcatagcc agaatctcgc
     6901 ccataaaccg cgtctccgaa tttgttttcc tcgcgaccaa ggattctctt tgttgaggca
     6961 atcggcggca tgccgaactt tggatcgcga tagttgtatt ctttcttttc ctcttctgga
     7021 ttggcttccg gcttagcaga ctcatcttct ggaatagaga gctgcggctc ttctgcattc
     7081 tgctgagagc gaaactgact cacagtagaa ggaacgccgc ctgacgttcc actcaagtcc
     7141 ttgatctttt cttttaattt ctcgttgcgc cagtaaaaat atccggcaat cagtatggcc
     7201 actacgattc caatttgata gaattcggaa tgcattcaga aacctggttc gcgccggtga
     7261 tctgtcgact tattttctaa gggcgtattc gcgctcgagc cgcgcaagca tttccccaac
     7321 acgcggatcg ccttcgcgcg gagcccacga agcaaactct ttatcgtcac ttggacggta
     7381 cggtccgggc ttgacttcaa agcaaacgca cctttccgac aagacaagaa gaccatgcca
     7441 gatgcccgga gcaatgtcaa ttccgcgaac aggaccatcc gcgctcaagg tgtggatttg
     7501 ttggaccact cctgagtcat caaaaatgaa gaacgcaatg ctcccttgca gaatcaaaaa
     7561 gctctccgct ttcggaggaa attggtggcg atgcgggcac aaataggttc ccttcaatag
     7621 cacgttgaga aaacgatgcg ggttgtcgtc catggagtca tggaaattat ggttgatgcg
     7681 aaggcgcggc tttgctctgg cctcggctgc cagcgaatcg aacatctctt cgcgaatcaa
     7741 ttgaataggt tcactcatgt cagaagtcct caagtcggaa gaaaatccat accgcacaca
     7801 gagcgcaagc ctggaatcag acggcagaac ggtctacctc tatctaaatc ccgtggaagg
     7861 tgtggacctg gtcccgcgcg ccgtgtgggt gatgaacctg gtgcccgctc ctgcagaaga
     7921 cgcggacgga atgatccgcg gccaggcacc tgtcagaagt cgtgctgcgt gcgcgcatcc
     7981 gaatggaatg aaagctccgc atgaaaacga aatagacttc gtctggtttc aggaaggcac
     8041 gggcgtcact ctctgtgtcg aaggtgaaat ctgggcagtc attccgccct tttccggttc
     8101 agatggattc tacggatttt gtcgcgaagc aactctgccc gagcacggca cgttcccgct
     8161 tccggatgga atgaaagcga gggcaaagga gaacctggat ttctggaacc gtcgcacgca
     8221 gaaaggtttc tgggcacagt ttcaggggaa tctactcaga acctatgagt ctgcattcgg
     8281 accgcataac aactattacg tgctagacaa cggcaagttt ccagccatgg gtctggctga
     8341 gtttatcatt ccagacacaa cccttcgcct ctttgccacg attggaatgt ccgcgcaaaa
     8401 tatgcctggt gtagaattgc atgtcaaaaa tccagagccg ctgtttcgtg tggaactact
     8461 gaccgttcgc gatgcgcccc atccctggtt catagatagc atgtctcgcg ttgcactcct
     8521 tccctggaga acaggtggct gggttggtcc gggccattcc attcaatttg gcaggtcgga
     8581 gcccatgtct gactatctgg tcctcgaaca ccacctggta caggacaaga ttcttgctga
     8641 tttgtttgcg aagctaccat ctgtattcct cgatgaacgt tatgcgcgaa ccttcctgtt
     8701 tctatggccg gccgtaccag acgattttcg cgtcaaccag gcaaggggga gagaatttct
     8761 cgcgaagaaa aacagcgtag ttgagcgttt ttcatgattt ctcttgatcc acgcttcgaa
     8821 gaagatttca aaaaaattcg cgactccggt tttgaaccag gccgggtggt ttctcaaacg
     8881 gtcacctacg aagtagcaac agaatcgggt gattacacag cgcaacccgc aggaaaactc
     8941 ctcttttcag gaatgcttcc agcagtgggc gactgggtgg cgctacaggt ttacgaggga
     9001 ggagccatca ttcatgatat agttcctcgc aaaaccgtga ttatgcgaaa atctgttggg
     9061 aaatcctccg ctgaccaggt gattgcggcc aatctggact acgccgtcgt ggtgatgggc
     9121 ctggactcaa acttcaatct gcgaagactg gaacgttata cgaccatcat tgccgcctca
     9181 ggcgcgaagg gaatcatagt tctgagcaag gcagatctcg cagatccaga ggaaatcaaa
     9241 ctgaaagtcg accacaccat tgcatgcgca cccggattgc ccgtcatcgt atggagcgat
     9301 gcagacaaac gcggcctgga agaactgatg gcaattctgc gtgggcccgc aactttctgc
     9361 ctccttggct caagtggcgc aggcaagtct acactcatca atgcaatcgc aggcaaaccc
     9421 atcgcaagaa ccggagctgt ccgcacatct gacgaaaaag gcaaacacac aaccacggtg
     9481 cgaactctca tttcgcttgg aaatggaatc gacttgatag acaaccctgg catgcgcgaa
     9541 attggactca cctctgaaga gggattatcg gatgcttttc cggaaatcac tcaggccgcc
     9601 gcagaatgca aattccagga ctgcacgcac gaaaacgaac ctggatgcgc tgtggaactc
     9661 gcggtacagg agggcagaat cactgcagaa cgattcgcct cctatatgag acttcttcac
     9721 gaatctcgca ctatagagtc tatgaaaagt gagcgagggc gtcagaagaa aaagcaaaac
     9781 gaaaaaactc ttgcgaaaat gattcgagcg cgtctcaagg ataagggtcg ttcatagaat
     9841 ccaggctgag tccgcctgtt tcccgacgtg gattgcgctg gatgcccgcg cgaagaacgc
     9901 tttcctttcc ataaatccac acaagctctt tggcgcgagt gagtgcggtg tagaggattt
     9961 gcctgctgac catgggatgg tctgattccg gaagccatag caacaccctg ttgtattcag
    10021 aaccctgaga tttgtggacc gttgtaacaa aagcaggttc acattcagga agggattcgg
    10081 gaggtagtag tctgatttcc tctccacggg cggcaatcca gcgaaaaccg gtgcgaaatc
    10141 gcacgaggag tccagtctct ccattgaata gttcgcgcga atagtcgttt cgagtgatca
    10201 tcagaggaag tccggaaaac cactctccga acattacgct tctgttttgc ttgaggaaaa
    10261 gtttcatcat cctccgattg agccgcacag acccgtcttc actctgtcgt agtgcactga
    10321 ggatgcgcgc gcgacctgtt acttcagtca aatgccgcat aacgtctacg gctgcaggat
    10381 catccgcatc cagagcagaa gcctctctca agagatctac aaactcaggt ccatacgaaa
    10441 gcttgagcca ggcgcccagc acttcattct ggccgccgtc ccgaaatgca atctgaacta
    10501 ccccgtgatt ctcgggcaat tcacggagag gaacaattga ccacggtaaa ctctcctccg
    10561 cttttcgcac agcatccgag gcgcgaatta caacgtcagc cgagcggtga ctggccgtca
    10621 ggtgaaccat ccggtccacc gcgcgacttt ttcctggcat ttgcggcaaa tccaactcag
    10681 gatccagggt acgcagccac gacgcgaagt ctggactgta ttgggggttg ccgctcagtg
    10741 cgaagagttc cacaagatca ccgagcacag cgcccgcgtc cacagacgga agttgattgc
    10801 gatccccgat cagaatcacg cgcgttcgcg ttggatccac cgctgcaagc aatcgcgaca
    10861 tgaggtccac gtccaccatg gagacttcat ccactaccag aacatctccg atcaatttgc
    10921 gattcggtcc aaaatagaaa tcttgagaat ccctcctgta agacaacgca gtgtgaatcg
    10981 tgctggccgt gtactggtcc cccagtgtct cagccattcg gcgcgcagca cgaccggtgg
    11041 gtgcgcaaag aatcacacga tccgctggaa tgcccgcgcg aaggaatgaa tcgaggactg
    11101 cacgaatcac ggttgttttt cctgtgcccg gcccgcccgt aatgattaca aaactactaa
    11161 agagcgacag ggccaccgcc agcttctgtt cagcactcag agtcaggcca gacaccgaca
    11221 gggcagtgcc atacgcctct ttcgctttct cgagagggcg cggaggctgc gccgccatgc
    11281 gtgcgaaatc tctctcgatg tttctacgaa gtaagaaata cttttgaaaa aatagaaatg
    11341 tatcagagag gaccaccggg cttttttcgt cttgtccgat caatggcgaa taacggttta
    11401 tcgatttcgc agccacaatc gcatcttcca gatttggctc cgtcgtgtca tgtctttgaa
    11461 caaaacgatc ccgcgttgaa gtgagcgaaa gatcatccaa tcgcatacac agatctccgt
    11521 gcatgcatgt gtccgcgaga agaagaagca aggctcgaat tgattcatcc cctggaaaat
    11581 gggagaactc gagcagatca tccacaagag aaacccagag gtagagctcc ggattttctc
    11641 tggccgcttc gcgaacggtt ctactcatac caggggctcc cctgcagaga aacgacgaat
    11701 gatgccggca agatcctcta gagcggccgg atctgggcgc acaaaataaa ccccatctgt
    11761 ttccccaggc ttcatgccac gcaagtagag atagaacaca ccgccaaacc gcgagggttc
    11821 gaattttccg ctggagttca gccacgaaat caatgcatga ccgtaaatag aatactgtag
    11881 atcatatcgc tcttctgcta tggacgtctg caaagacgat tcgctgtagt cgggaagagt
    11941 atttgttttc caatccaata tgtagtacgt atctttcacc cggaagacaa ggtctatcgc
    12001 accatgaatg tatccatcgc gaaccagaag attcttatct ccggaaaaac cttgagcgcg
    12061 tgcgagaaat cgaaattcgg ggactttgtg cttcactttg cataatcggc caattccctt
    12121 taaatccgcg ttgagtgctg aatgaatcat ggcgagcgcc gcgcgaaaca tgtcttctcc
    12181 tacgagtccg tattcgttca ggcacgcctg aatgtgatcg cgtgttgctg gctcaagggt
    12241 gcccggtttt ggtacttgct tcctgtcaaa ttcagaaaaa tccagaccct caagtgcctc
    12301 gtggatcgca ttgcccgtcg cttctccccg cggaagctcc ggatgatcca cgatcaggaa
    12361 attacctttt tgatcgagcg ttggatcgcg cgtctccgga agaggaagag gttcatcttc
    12421 cgccgggttt gtatctccaa actcagcacg aagcgcatcc agagcaacag ctcgcctgag
    12481 aatcccagag taagacgtga gccccgctgc acgcacagat aggttggtag gaaagcggct
    12541 caattctgtt cttcccggtg ctttggcggt tcgggtcagt ggttcaggag gacctgcgtg
    12601 catgggctga accaatcgcg ccaggttttg cgcaagaacc tgatccgcgg caggcgctac
    12661 aaggtgagac aatggaccgc ctcttccact gggaaagaag ggggcaaaca ccctgtctcg
    12721 cgctcttgta aaagctacgt agtacaatct tttatattct tcaaacctct ggattt
//
LOCUS       DAORFF010000023        12642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2159_length_12642_cov_3.324938, whole genome shotgun sequence.
ACCESSION   DAORFF010000023 DAORFF010000000
VERSION     DAORFF010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12642
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2159_length_12642_cov_3.324938"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PKK76_01895"
     CDS             <1..811
                     /locus_tag="PKK76_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNL67241.1"
                     /translation="RASFMEMVLGGSAFFGLGVFFAGLAVTYWFFRKIPPAVFFESLG
                     LIDPERQRLLLESAVLYVFLVFPVGAAFGKQVAHLFPNATLFALVHLTIAFFASCGYY
                     ALNAGPGSLRMLAVGQVTDPMKEVAVGVMGFAAIFPPALLALLLTMGSAAQDSVRIAH
                     PISFEIQKNPVLVFILAAVLVPIFEEVIFRCFLYGFFRREQRIRYAAFFSGSIFAILH
                     PQGAIALPYLTILGMGLAVLREYRPSPLASMTAHACVNGTAVILGYAFTAL"
     gene            complement(808..1611)
                     /locus_tag="PKK76_01900"
     CDS             complement(808..1611)
                     /locus_tag="PKK76_01900"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HNL67242.1"
                     /translation="MTGVFVERIIVYPIKSLDGVSVSECRITDGGSLEHDRLLAIVDE
                     QGTFVNAKRFAKIHAIRAVYTEDFRAVTLSAPDKPAHQFSMEDRDRMSLWFSDFFEMN
                     LRIMQNTQTGFPDDLEASGPTFVASATLQRVCEWFAASGQGFKFEEAIRRFRPNVLFG
                     GADSFYEDQLYGGATFFAGDVTVVGSNPCARCAVPSRHPSTGEVIQGFSKTLSDARKK
                     HLPATSNPDHFSHYYRLCLNTRIHSSETGKTLRVGDAVQTEWDSWGKIF"
     gene            complement(1620..2168)
                     /locus_tag="PKK76_01905"
     CDS             complement(1620..2168)
                     /locus_tag="PKK76_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67243.1"
                     /translation="MNKESDKPKQEPSPDQNRSEKPTQDEKKEKAYVVESRITLGDGR
                     IVNGRLKFNAPEKITLVHEAEGIQYEKVIGMGEISSIEILKWKGKFLRENKNGMVYEF
                     AAEEFRIRLKDGHELRKSGGLFPFLRKIPLENKNGQIQVFTFWMDLKKPDGTWYTGIA
                     GPDTGLRNTCHPDVIRRIDFEK"
     gene            complement(2272..3348)
                     /locus_tag="PKK76_01910"
     CDS             complement(2272..3348)
                     /locus_tag="PKK76_01910"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HNL67244.1"
                     /translation="MALAGVYRTERKIQPIEAQRPSLSKDELESVLDCLIHDRLMSGS
                     VAGRFEKSFAHAFGFKHALSVNSQTAAYHLAYLALGVGAGDLVAMSSIAPVGAYDAAR
                     YTGADVALVDIERGSFHPSQDAVQAALSREAGKKIYILDHTFGAPASLEFPGENVQII
                     ADFTGLVGAEIQASQFGALSICGMSQYDLMTTGNGAIIVTNDSKLFQKIDSLRYGSKR
                     QEGSIAYDYRLEDFQAAIGLEQLTRLAVTLARRRKIGVKYIESLQRTQHETYFKTPGT
                     DCYLRFPIVVSRPHDEVIRYFNSLQIGVTRAVDLPLHHLAGLPRLEYPNAERLYQKSV
                     SVPVYPALSANNVERIAQSLRGLL"
     gene            complement(3492..4616)
                     /gene="manA"
                     /locus_tag="PKK76_01915"
     CDS             complement(3492..4616)
                     /gene="manA"
                     /locus_tag="PKK76_01915"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HNL67245.1"
                     /translation="MSRRSHYLLKCPVQKYHWGSLDAESILRRIAFKAHEAVLEDEPA
                     AELWMGAHPTAPSIVQPENESLASLIATEPDYFLGHGGHLSFLFKILHADRPLSIQAH
                     PDRTLAKQLHARDAKNYPDPNHKPELAMCIQDMRALVGFRNENEIRVELERHAALLEI
                     CGHIEDGVRGWYAGLMRTDGEKVARAAERVRSAVSREPEEICFLDLCGIYGDRDPGIF
                     APFFLNYMELAHGEAIFLGPNEPHSYLGGEILECMAASDNVVRAGLTTKYCDVDTLVS
                     MLHYRTGPHQIVKPVQEANGLRYPVPVNDFALHKINQPIHRRGPAIVLALKGTMEING
                     EIFPSGSAVFIPHGQPSTLAPASGSEIYSCSTPEDFNPPD"
     gene            complement(4613..4816)
                     /locus_tag="PKK76_01920"
     CDS             complement(4613..4816)
                     /locus_tag="PKK76_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67246.1"
                     /translation="MQRFFTILKYDFLALVPGSTVPFSLHAMAKLILYAGFILFVFFL
                     FYRLPVRIHEPEQPRQPSIERPI"
     gene            4896..5873
                     /locus_tag="PKK76_01925"
     CDS             4896..5873
                     /locus_tag="PKK76_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67247.1"
                     /translation="MRFCILIGLLCFCGPLFSQPVADTSVDQVSGPDGRFRVLRIFPM
                     HYWLDSEQESNVRALTEASGPGDPLVVVLWGPYTPRVLDANAERSRIVPQHFEWFLHR
                     LCYKDVNYEDPAYRKHQMAESRCYAERTVEFLKGRTLPYFMFAMWGDNLESYEYIGLI
                     RYPVILLTMVVFSIPQLLLFLLILLPFKSWLSSREFVQRVFKVVLPMIIGIAWLFFFM
                     ERVGDEWYITPFRFVLPMSGVYFALTFISCMWIADYVYFRQNNPPSPWVVAFFYVFAP
                     IMILGAGAAARGSRGTYVSSRGSSSGTSGGSGSTGGGGSFGGGGASASF"
     gene            complement(5874..7613)
                     /locus_tag="PKK76_01930"
     CDS             complement(5874..7613)
                     /locus_tag="PKK76_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNL67248.1"
                     /translation="MEWDQAIFKKVLTLAGRLKKKGTLNPNRSILSELKDRLTIVSRM
                     LTGDPVEIVTAEAGGGFRNQFFFLPAFYEGFESRADNDLFFFLRVCCLAEQRRMGLNW
                     SKDGQTEEESISRATGSLEAILSAVAKKYPSLRLTIDSVIRTELATNGSLKLLAGKWM
                     APIESSGGEVTPGSRNTQSQKEQKEKVTEIDGKTREDVRTHTPDTKAIEDYTLSHNFE
                     KAETIEEFNGTWRSMDGEDDLSDHEEALKELDLRDTVRVDTPAHSVYRTEFFVNASAP
                     ESASSTEEGTFIPYDEWDYKIRSYKKAHCRIYPGRPRNLSADYVRSALDQDRGTLARL
                     RRTMSSVNNEMEQVRRQSSGEEPDLDALVDNFSELAAGKTPSEKVYLSRRKRKRDLAV
                     LILLDISLSTDAYTGGERILDVEKRSVALLSEILAESEDDFAIDAFFSKTRNHISYLN
                     VKRFSEGWAPARGRLGGLEATGYTRIGPAIRHATAEILSRSARKKWILLLSDGKPNDY
                     DRYEGRYGMADVRRALKEARNADVHTFALAVEASARHYLPLMLGQGRYRILPHPSMLP
                     EALTEFYARLRAG"
     gene            complement(7634..8431)
                     /locus_tag="PKK76_01935"
     CDS             complement(7634..8431)
                     /locus_tag="PKK76_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNL67249.1"
                     /translation="MPQNADDANGLPYYKPVGRECEVFEHAYKNRLPLLLKGPTGSGK
                     SRFVEYMSAKMKLPMITVSCHDETSAVDLLGRYLVKGSETTWQDGPMTRACRTGAVLY
                     LDEIAEARPDTIVALHSLTDHRRILYLDRHDEEIAAPPTFMLIASFNPGYQKGWKELK
                     ASTRQRFVSIEFQYPSEAVESEIVRAESNLDLSQVKLLVQLAGRIRNLAELGLAETVS
                     TRLLVSTAKLMADGLPARLACDVGIVQPLTDDAETGAAVRDVVALVF"
     gene            complement(8412..9785)
                     /locus_tag="PKK76_01940"
     CDS             complement(8412..9785)
                     /locus_tag="PKK76_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNL67250.1"
                     /translation="MKYKSQKIAYWFFATCMLLFSLQIIYGFVMGFARIGMDGLHDFI
                     PFNAARATHTNLLVVWLLTGFMGAAYFIIPDESGRELFSVKLAWVQLISLIVVGVVAI
                     IGFHLNYWEGRKFLEIPRPLDFLVVVNVLMFIFNIGMTILKGNRYSTTQLVLFIGLLS
                     AALLYLPGMIYFRSQTMDSYFRWWVVHLWVEGVWELIMGSILSYLLIKLTGVDREVIE
                     KWLYVIVGLTFLSGLLGTGHHYYYIGTPKYWLMVGGIFSALEPLAFLGMALFAINMYR
                     KSGREHPNKISLYWTIGCSIMSFVGAGFLGIAHTIPQVNLWTHGTLVTAMHGHMAFWG
                     AYAMIVLAVITYCLPLLTGRKLWNNLPGLLSFWLANIGMTAMTGAFAVAGITQVYLER
                     KMGMDFLTVQKEIQIHFVGLIAAASLFTIGAILFIYNFIKFGLPSDEAIVPENTSDEA
                     LYAAKRG"
     gene            complement(9801..10481)
                     /locus_tag="PKK76_01945"
     CDS             complement(9801..10481)
                     /locus_tag="PKK76_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNL67251.1"
                     /translation="MLSKSQAKLFFLAGTALCAGLFLFLTVDTMRKIPEQTHEANITP
                     EVERGKEIWDKNNCMGCHTLLGEGAYYAPELTKVYDRRGAEWMKVFLKDPQAMFPGER
                     KMVKYNFSDQDITDLIAFFKWIGEMDLNGFPPKPNLHMTSSAATGPAVETPAKFKQLC
                     VACHSLGGAGGNVGPALDGVGSRRDLAYIEKWLRDPSAIKPGAKMPKLALSEAEIKEI
                     STFLAGLK"
     gene            10592..11485
                     /locus_tag="PKK76_01950"
     CDS             10592..11485
                     /locus_tag="PKK76_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNL67252.1"
                     /translation="MNDSPSPPSHSRAFLGFLFLIAIVSLGHASLFARAAQAPPEVVG
                     FWRMSGAALILFPFALRYANVNRKQILLSFIAGFFFFIHLWTFIFAAQHTKIANCVVL
                     FAVNPVFTSIGHWFLDGVRPRWKTLFAYAAAGAGVLIALHNKTSFAAFSLQGDLLAVV
                     SAGFFSAFILTGNAARKNLNNLVFSPIVYFSAAVCFLVCAVLRSIPLLPWPLESWAAI
                     LGYILVPTLLGHTLYTYLLSHVRIHVMSFGKMIEPLVAIVSAWFLFSEKPGEEILLSF
                     VFTAVATLLILWPDQNDPRRV"
     gene            11466..11837
                     /locus_tag="PKK76_01955"
     CDS             11466..11837
                     /locus_tag="PKK76_01955"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNL67253.1"
                     /translation="MTLEEFEQIVEEERLALPREFADLLDLHNVAIMVDDWPLPEILR
                     TAGIRNPSSLFGFYSGIPLPDRGADKPLLPDRIWIFREPILRAVSTDAQIRAEIRTTL
                     RHEIAHFFGFSDEHLLREGTY"
     gene            12003..12437
                     /locus_tag="PKK76_01960"
     CDS             12003..12437
                     /locus_tag="PKK76_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNL67254.1"
                     /translation="MNRAIKALKYAVTVGLFFAGASLFAQASPVGSWKTIDDETGKPK
                     SIVNIWEQGGVLYATIADLINPDEPDPKCTKCTGDYKDKPIKGMTIMWGMKKDGDSWS
                     GGQILDPKKGQTYSCKLWLEGSNLKLRGYLGPFYRTQTWYRN"
BASE COUNT         2808 a         3160 c         3240 g         3434 t
ORIGIN      
        1 gcgcgcgagt ttcatggaaa tggtgctggg tggaagcgca ttttttgggc tgggcgtatt
       61 ctttgcgggt ctggccgtca cctactggtt ctttagaaaa atcccccccg ctgtgttttt
      121 tgaatccctc ggactcatcg atcctgaaag gcaacgtctc cttctggaat cagctgtatt
      181 gtatgttttt cttgttttcc cggtgggagc ggcttttggc aagcaggtag cgcatttgtt
      241 cccgaacgcg acgttatttg ctcttgttca tctcacgatt gctttctttg cttcttgcgg
      301 atactacgca ctcaatgcgg gcccagggtc tctgcgtatg ctcgcggtcg ggcaggtcac
      361 cgatccaatg aaagaagtgg ccgtgggagt catggggttt gctgccattt ttcctcctgc
      421 attgcttgct cttcttctga ccatgggatc tgcggcccag gattccgtgc gaatcgctca
      481 tccgatttct tttgaaatcc agaagaatcc ggttcttgtt tttattcttg cggcagtcct
      541 tgttccgatc ttcgaagaag tgatctttcg ctgttttctc tacgggttct tcagacgaga
      601 gcagcgcatt cgctatgctg catttttttc agggagcatt tttgcgattc ttcatcctca
      661 gggtgccatc gcactgccat acctgacgat tctgggtatg ggactggcag tactgcgaga
      721 gtatcggcct tctcctctcg catccatgac tgcacacgca tgcgtcaatg gcacggctgt
      781 aatactggga tacgctttca cggctctcta gaagattttg ccccaggaat cccattcagt
      841 ctgcaccgcg tctcctacgc ggagagtctt cccggtttct gacgaatgga ttctcgtatt
      901 caaacacaat cgatagtagt gcgaaaaatg atccgggttt gaagtggccg gtaagtgttt
      961 ctttctggcg tcactgagag tttttgagaa accctgaatg acttctcccg tagatggatg
     1021 tcgtgacggc acggcacacc gggcgcacgg attgcttccg accacggtta catctcctgc
     1081 gaaaaaagtg gcacctccgt agagctgatc ttcgtagaaa gagtctgcgc ctccgaaaag
     1141 aacattgggt ctaaagcggc ggatcgcttc ttcgaatttg aatccctgac cgcttgcagc
     1201 aaaccattcg catacccgct gcaaagtggc agaagcaaca aacgtgggac cagaggcctc
     1261 tagatcgtct ggaaaaccgg tctgagtgtt ttgcatgatg cgaaggttca tttcgaagaa
     1321 atcactgaac cagagagaca ttctgtctct gtcttccatt gaaaactgat gcgctggttt
     1381 gtcgggcgcg cttagagtta ctgctcgaaa atcttctgtg tagacggcgc gaatcgcgtg
     1441 gatcttcgcg aagcgttttg catttacgaa cgttccctgt tcgtctacga tcgccagaag
     1501 tctatcatgt tcgaggctac ccccgtccgt tatgcggcat tcggagacag atactccgtc
     1561 cagtgacttg attgggtaga cgatgatcct ttcaacaaag acgcccgtca tgcctgttgt
     1621 tacttctcaa agtcgatgcg acgaatgaca tccggatggc aagtgttgcg aaggcctgtg
     1681 tccggtcccg caatgccagt ataccacgtg ccgtctggtt tcttgagatc catccagaac
     1741 gtaaagacct gaatctggcc attcttgttt tctagtggaa tttttctcag gaatggaaac
     1801 agtccgcctg atttgcgaag ctcatggcca tctttgaggc gaatgcgaaa ttcttctgcg
     1861 gcaaattcgt agaccattcc gtttttgttt tcgcgcagaa atttcccttt ccatttgagt
     1921 atttcaatcg aggaaatctc acccattcct atcactttct cgtactggat gccttctgct
     1981 tcgtgaacca gggtgatctt ttctggagcg ttgaatttga gtcgtccatt tacgattctg
     2041 ccatctccca gagtgattct ggattcaact acgtaggctt tctctttctt ttcgtcctgg
     2101 gtcggctttt cgcttctgtt ttgatccgga gatggctctt gctttggctt gtccgattcc
     2161 ttattcacgg gagtcgcgga cgttatttgc ttttgattgc gaggctttgc gtcagcggca
     2221 accgtgacgc atacaaaaac cgcacagaat gcgaagggca gagaacctgg cttagagaag
     2281 gccacgtagg gattgcgcaa ttcgttccac attgtttgcc gacagtgcag ggtaaaccgg
     2341 cacagaaact gatttttgat acaatcgctc tgcatttgga tattcaagac gcggcaagcc
     2401 tgccaggtga tgaagtggga ggtccactgc gcgagtcaca ccaatctgca atgagttgaa
     2461 gtaacgaatc acttcatcgt gcggtctgga aacaacaatt ggaaagcgaa ggtagcagtc
     2521 tgttcccgga gttttgaaat aggtctcgtg ttgagttctc tgcagagatt caatgtattt
     2581 aacgccgatc ttgcgtctgc gcgccagtgt cactgcaagc ctggtcaatt gttccagacc
     2641 aatggctgct tgaaaatctt ccagcctgta atcatatgcg atggatcctt cctggcgttt
     2701 ggatccgtag cgaagagaat ctattttctg aaaaagtttt gagtcgtttg ttacgatgat
     2761 cgcaccgttt ccagtcgtca tgagatcgta ttgagacatg ccacagatgg acaacgctcc
     2821 gaattgagat gcctggatct cggcccccac aaggccagtg aagtcagcga tgatctgaac
     2881 gttttcgcct ggaaattcca gagatgcagg agcaccgaat gtatgatcca gaatatagat
     2941 tttctttcca gcttcgcgcg aaagtgcagc ctgcaccgcg tcctgcgatg ggtgaaagga
     3001 gccgcgctcg atgtcgacaa gtgcaacgtc tgctcctgta taccgggctg catcgtaagc
     3061 tccgactgga gcaatcgatg acatggcaac caggtccccg gcaccgacgc ccagtgccag
     3121 ataagccagg tggtaggctg cggtctgtga gttcacgctc agcgcgtgct taaaaccaaa
     3181 tgcatgtgcg aatgactttt caaaacgacc ggccacgctg ccgctcatca gtctgtcatg
     3241 aatcaaacaa tcaaggacgg attccagttc gtccttgctc aggctcggtc tttgagcctc
     3301 tatcggttga atttttcttt ccgtcctgta taccccagcc aaagccacaa ttctagtcct
     3361 gtttatattt cgtcctttgt aagatattat gtcacattgc gtccagccct ttttagcttt
     3421 tgcggcaaaa tttagaactc cctgatttga cgttccgccc gggtccgggc ggtcagttgg
     3481 ttttcaaaaa ttcagtctgg aggattgaaa tcttcaggtg tagagcagga gtaaatttcg
     3541 gaaccggaag cgggtgcaag cgtagaaggc tgcccgtgcg ggatgaacac cgcgcttccg
     3601 gatggaaaga tttcgccgtt tatttccata gtacctttca atgccagcac aatggcgggg
     3661 ccgcgtctat ggattggttg gtttatttta tgaagcgcga aatcgttcac tggcacggga
     3721 tatctgagcc cgtttgcttc ctgaactggt tttacaatct ggtgcgggcc cgtgcgataa
     3781 tgcagcatgg atacaagagt atccacgtcg cagtattttg tagtgagtcc ggcgcgcact
     3841 acattgtctg acgcagccat gcactcgagt atttctcccc ccagatagga gtgaggttca
     3901 ttggggccaa ggaagatagc ttccccgtgc gccagttcca tgtaatttag aaagaaaggt
     3961 gcaaatattc cagggtcgcg atctccatag atgccgcata gatccagaaa gcagatttct
     4021 tccggttccc gggaaaccgc agagcgaact ctctccgccg cacgagccac tttttctccg
     4081 tctgtgcgca tcaatcctgc gtaccagccg cgcacaccat cttcaatatg tccgcatatc
     4141 tcgagcaatg ccgcatgacg ttccagttca acgcgaattt cgttttcatt tctaaatcca
     4201 acaagcgcgc gcatgtcttg aatgcacatg gcaagttctg gtttgtgatt cgggtccgga
     4261 tagttctttg catctcttgc gtgaagttgt tttgccagtg tgcgatccgg atgggcctga
     4321 atggaaagtg ggcgatctgc gtgcaagatt ttgaaaagaa agctcaggtg tccgccgtgg
     4381 cccaggaaat agtcaggctc agtagcgatc agagatgcca ggctttcatt ttcaggttga
     4441 acgatggacg gcgcagtagg atgggcgccc atccacaact cagccgctgg ttcgtcttcg
     4501 agtactgcct catgagcctt gaaggcgatg cggcgcagga tgctttccgc atccagggag
     4561 ccccagtggt atttttgaac gggacacttc agtagataat ggctgcgtcg actcaaatag
     4621 gcctctctat ggaaggctgg cgcggctgct cgggctcatg gatccgaaca gggaggcgat
     4681 agaaaagaaa gaacacgaac aatatgaatc cagcgtacag aatcaatttt gccatcgcat
     4741 gaagcgaaaa ggggactgtg ctgcccggaa cgagggccag aaaatcatat ttcagaattg
     4801 taaagaatct ctgcacgatt ccagcgtgta aggcgtgttt ttgcggtcaa acttattcga
     4861 gttgaaatcc tatacggcga gagagagaac tgctgatgcg tttttgcatt ttgatcggtc
     4921 tactttgctt ttgtggcccg ctcttttcgc agccagtggc agacacttct gtggaccagg
     4981 tttcggggcc agacggcaga tttcgcgtgc ttcgcatttt cccgatgcac tactggcttg
     5041 attcagagca ggagtcaaac gtgcgcgctc tgacagaagc atcagggccc ggcgatcctc
     5101 ttgtggttgt gttgtggggg ccctatacgc ccagggtgct cgatgcaaac gctgagagat
     5161 ccaggatcgt tccgcagcat tttgaatggt tcttgcatcg gctgtgttac aaagacgtga
     5221 actacgaaga tccggcttat cgcaagcatc aaatggcaga aagtcgctgt tatgcagagc
     5281 gcaccgttga gtttttaaaa ggcaggactc tgccgtactt catgtttgca atgtggggcg
     5341 ataatctgga gtcctacgaa tacatcggtc tgattcgcta tccagtcatt cttcttacta
     5401 tggtcgtgtt ctctattcca caattgttgc ttttcctcct gatccttcta ccattcaaat
     5461 cgtggctttc gagtcgtgaa ttcgtgcagc gagtctttaa ggtagtgctc cccatgatca
     5521 ttggaattgc ctggttgttt tttttcatgg agagagtggg agacgagtgg tatatcacgc
     5581 cgtttcgttt tgtgcttcct atgtctggag tttattttgc cctcactttc atttcgtgca
     5641 tgtggatcgc agactacgtt tactttcgcc agaacaatcc gccatccccc tgggtagttg
     5701 cattttttta tgtttttgct cccatcatga ttcttggagc gggtgcggcc gcccgtggtt
     5761 ccaggggaac atacgtctct tctaggggtt ccagttccgg gacgtctggc ggttccggct
     5821 ccaccggcgg aggcggaagc tttggtggag gaggagcgag tgcgagcttt tagttagccg
     5881 gcgcgcaatc gtgcgtagaa ttcagtgagc gcttccggga gcatggaggg atgaggcagg
     5941 attctgtatc ttccctggcc cagcatcagt ggtaagtaat gtcgcgcgga tgcttctacg
     6001 gccagcgcga atgtatgaac atccgcattc cgcgcttcct tgagcgctcg tctgacatct
     6061 gccattccgt agcgtccttc ataccggtca tagtcgtttg gtttcccatc agatagaagc
     6121 agaatccatt ttttccgagc gcttcgcgag aggatttcag ccgtggcatg acgaatcgcc
     6181 ggaccaatgc gcgtgtatcc tgttgcttca agcccgccga gtcttccgcg cgcaggagcc
     6241 cacccttcgg aaaagcgctt aacgttgaga taggagatat gatttcgcgt tttagagaag
     6301 aacgcatcga ttgcaaaatc atcttcagac tctgcaagga tttctgaaag cagcgccacg
     6361 gaccgttttt ccacatcgag tattctttct ccgcctgtgt atgcgtccgt ggacaggcta
     6421 atgtccagaa gtatcaagac cgcgagatct ctttttcttt tgcgccggga caggtagact
     6481 ttttcggatg gtgtttttcc ggccgcgagt tctgaaaaat tgtccaccag tgcgtccagg
     6541 tcgggctcct ctcccgaaga ctgtctgcga acctgttcca tttcattgtt tacggatgac
     6601 atggttcgcc tcaggcgagc cagggttccg cggtcttgat ccagtgcgga tcgcacgtaa
     6661 tctgcggaca ggtttcgcgg acgacccgga taaattctgc agtgtgcttt tttgtaggat
     6721 cttattttgt aatcccattc gtcgtaggga ataaaggtgc cttcctctgt agagcttgca
     6781 ctttctggcg cggaggcatt tacaaagaac tcggttctgt aaacggaatg tgccggtgtg
     6841 tccactcgaa cggtgtcgcg caaatccagt tccttgagag cttcctcgtg gtcgctcaga
     6901 tcgtcttccc cgtccatgct tctccatgtt ccgttgaatt cctcgatggt ttccgctttc
     6961 tcgaagttat gcgatagtgt gtagtcttca attgcctttg tgtccggagt gtgcgtgcga
     7021 acgtcctctc ttgtcttgcc atcaatctcc gtaacctttt ccttttgttc tttctgcgat
     7081 tgtgtgttcc gggaacccgg cgtcacttct ccgccgctag attcaatcgg ggccatccat
     7141 tttccagcca gcaacttcag gctaccgttt gtggcaagct ccgtgcgaat cacagaatca
     7201 atggtgagac gcaaagatgg atatttcttc gccaccgcgg aaagaattgc ttcgagtgag
     7261 cctgttgcgc gcgatatgga ttcttcctct gtttgtccat ctttcgacca gttgagtccc
     7321 attctccttt gctctgcaag acagcatact ctgaggaaga aaaagagatc attgtctgcg
     7381 cgagattcaa atccttcgta gaatgcgggc aaaaagaaga actgattcct gaatcctccg
     7441 cctgcctccg cagtcacgat ttccacggga tccccggtga gcattcgtga aacaatcgtt
     7501 aggcggtctt tgagctctga caggatggag cgattgggat tgagtgttcc tttctttttg
     7561 aggcggccgg cgagcgtcag caccttcttg aagattgcct gatcccactc cattgtaatc
     7621 tctggaagcg ccgttaaaac accagggcta caacgtctcg gacggcggcg ccagtttctg
     7681 cgtcatctgt gagcggttgc acgattccta cgtcgcaggc gagtctagcc ggaagtccgt
     7741 ctgccatgag ctttgccgtg ctcaccagca atctagtgga tacagtttct gccagtccga
     7801 gttccgccag atttctgatt ctacctgcca gctgcaccag cagcttgacc tgagataaat
     7861 ccagattgga ttctgcgcga acgatttcag attccaccgc ttccgagggg tattgaaact
     7921 cgatcgatac gaagcgctgt cgcgtggatg ctttgagttc tttccatccc ttttgatagc
     7981 caggattgaa agaggcgatc aacatgaaag tagggggtgc tgcaatttct tcatcatgtc
     8041 tgtccagata gagaatgcgt ctgtggtccg tgagtgagtg aagtgcaacg atggtatccg
     8101 gtcttgcttc cgcaatttca tccaggtata gaaccgcgcc agttctacac gcgcgcgtca
     8161 tgggtccgtc ctgccaggtt gtttcagatc ctttgaccag ataacgtccg agtaaatcca
     8221 ccgcagaagt ttcgtcgtgg caggaaactg taatcatggg cagtttcatt ttcgcggaca
     8281 tgtactccac gaatctagac ttgcccgaac ccgtgggccc cttcaaaaga aggggcagac
     8341 ggttcttgta agcgtgttca aaaacttcgc attcgcggcc taccggttta tagtacggca
     8401 ggccgttggc gtcatccgcg ttttgcggca tagagggctt cgtcagatgt attctccggt
     8461 acaattgctt catccgacgg caggccgaat ttgatgaagt tgtagataaa gagaatcgcg
     8521 cctatggtaa acagactcgc ggctgcgatc aatccaacga agtgaatctg gatttccttt
     8581 tgaactgtaa ggaagtccat tcccatttta cgttcgagat acacctgcgt aattccagcg
     8641 acggcgaacg cgcctgtcat cgcagtcata ccaatgtttg caagccagaa tgaaagaagg
     8701 cccggcagat tgttccagag ctttcttcca gtgagaagcg gcaaacagta cgtgatcacc
     8761 gcaagaacaa tcatcgcgta ggctccccag aatgccatat ggccatgcat cgccgtaacc
     8821 agcgtgccgt gggtccacag gttcacctgc gggatcgtat gcgcaattcc caaaaatccc
     8881 gcaccgacaa aagacatgat ggagcaaccg attgtccaat agagtgaaat cttatttgga
     8941 tgttctcgac cgctcttgcg atacatgttg atggcaaaga gcgccatccc aaggaaagcg
     9001 agtggttcga gagctgagaa gattcctcct accatcagcc aatatttagg agtgccaatg
     9061 tagtagtagt gatggcctgt tcccagtagt cccgaaagga acgtgagtcc tacgatcaca
     9121 tacaaccact tctcaatgac ttctctgtct acgcccgtca gtttgatgag caggtaagaa
     9181 aggatggatc ccatgatcaa ttcccagaca ccttctaccc acagatgaac gacccaccag
     9241 cggaaataag aatccattgt ctgactgcga aagtagatca tccctggaag atacaacagc
     9301 gccgcagaaa gaagtccaat gaacagaacc agctgcgttg tcgaataacg gttccctttc
     9361 aggatggtca tgccaatgtt aaagatgaac atgagcacat tgaccaccac gagaaaatcg
     9421 agcggtcttg ggatttccag aaacttgcgg ccttcccagt agttcaggtg gaatccaatg
     9481 atcgccacca cacccactac tatcaaagag atgagctgaa cccacgcgag tttgactgaa
     9541 aatagttcgc ggccggattc atctggaatg atgaagtagg ctgctcccat aaaaccggtg
     9601 agaagccaga ccaccaggag gtttgtatgc gttgctcttg ctgcgttaaa cggaatgaag
     9661 tcgtgcagtc cgtccatacc aatgcgcgcg aagcccatta cgaaaccgta aataatctgg
     9721 agtgaaaaca gaagcatgca ggttgcgaag aaccagtatg caattttttg cgatttatat
     9781 ttcatctaga tcctcccgac ctacttcaat cccgcaagaa atgtggagat ttctttgatt
     9841 tcggcttcgc tgagtgcgag tttgggcatt ttcgctccgg gctttattgc agatggatcg
     9901 cgtagccact tttctatgta ggcgaggtct cgcctggatc caactccatc tagagccggg
     9961 ccaacgttgc cgcctgctcc gccgagtgaa tgacatgcta cgcagagctg cttgaactta
    10021 gccggtgttt ctacggccgg tccagtagcg gcggaggaag tcatatggag atttggtttt
    10081 ggtggaaatc cgttcagatc catttcgcca atccatttga agaatgcgat gagatcggtg
    10141 atgtcctgat cggaaaagtt gtatttcacc atcttacgtt cgccggggaa catggcctga
    10201 ggatctttca agaagacctt catccattcc gctccgcgtc tgtcgtaaac tttggtgagt
    10261 tcaggtgcat agtatgcgcc ttctccaaga agtgtatggc aacccataca gttgttcttg
    10321 tcccagattt ctttgccccg ttctacttcg ggggtgatgt ttgcttcatg agtttgctcg
    10381 gggattttgc gcatagtgtc gaccgtcaaa aagaggaaga ggccggcgca gagcgcagta
    10441 ccagcgagga aaaacagttt tgcctgtgat ttgctaagca tggatactcc ggggtactag
    10501 cccgcggcca gcataacatg gcgaaaacgg aaaacccttg atttatatca agtattgaat
    10561 ttttttgtcg ccaggtgctg cgaaagttat tatgaacgat tcaccttcgc caccttcgca
    10621 ctcgcgtgcg ttccttggat ttctatttct aatcgccatt gtttctctgg ggcatgcttc
    10681 tttgtttgcg cgcgcagcac aggctcctcc agaggtggtt ggtttctgga ggatgagtgg
    10741 cgcggccctg atcctttttc catttgcgct tcgctatgcg aacgtgaatc gcaaacagat
    10801 ccttctctcc tttatcgcag gattcttctt ttttattcac ctatggacgt tcatatttgc
    10861 ggcacagcat actaagattg cgaattgtgt ggttctattt gcggtcaatc ctgtgttcac
    10921 gtcgattggc cactggttcc tggacggagt tcgcccacgc tggaaaactt tgttcgccta
    10981 cgcagccgca ggtgccgggg ttctgattgc tcttcacaac aagacttcgt ttgcggcgtt
    11041 ttcgcttcag ggcgatttgc ttgccgtggt atccgctgga tttttttcag cgttcattct
    11101 aacgggcaac gctgccagga agaacctgaa caatctggtg ttttcaccca ttgtgtattt
    11161 ttcggcggcg gtatgctttc ttgtgtgcgc cgtcctgcga tctattccgc tcttgccctg
    11221 gcctctggaa tcatgggctg cgattctggg atacatcctt gtgccgacct tgctcggaca
    11281 cactctctac acctatttgc tatctcacgt tcgcattcac gtgatgagtt ttggaaaaat
    11341 gatcgaacct ctcgttgcaa ttgtttctgc gtggttctta ttttctgaga agccggggga
    11401 ggagatccta ctttcctttg tgtttacggc tgtggccaca ttgctgattt tgtggccgga
    11461 tcagaatgac cctcgaagag tttgaacaga ttgtagaaga agagagactc gcccttccgc
    11521 gagaattcgc agatttgctc gatttgcaca atgtggctat catggtggat gactggcctt
    11581 tgcctgagat cttacgcacc gccgggatta gaaatccatc cagtcttttt ggcttctatt
    11641 cagggattcc tctcccggat cgaggggcgg acaagcctct tttgccggat cgaatctgga
    11701 tctttcgcga gccgattttg cgggcagtct ccacagatgc gcaaattcgc gccgagattc
    11761 gcacaacact gagacatgag attgcccact tctttggctt cagcgacgag cacctacttc
    11821 gcgagggtac atactgattc cagtgtctga cgcacccgct gacccaccgc agtgcaggaa
    11881 agttcacgga aaatcacctt cctgggaacc aggaaaattc ttgttgcctc cccggataca
    11941 gaacctaatg acaattcgcc gcgtccaaag cggtgtgtac gtaaaaaaaa atggagggtg
    12001 tcatgaaccg agctatcaaa gcgctcaagt atgcggtcac agttggactg tttttcgcag
    12061 gtgcaagtct gttcgcgcaa gcgagtccag tcggcagctg gaagacaatt gacgacgaga
    12121 caggaaagcc aaaatcaatt gtaaatatct gggagcaggg cggcgtactg tacgctacga
    12181 ttgcagatct gatcaatccg gatgaaccgg atccaaaatg caccaagtgc acaggagact
    12241 acaaagacaa gccaatcaaa ggcatgacca ttatgtgggg catgaagaaa gatggcgatt
    12301 cctggtcagg tggtcagatc cttgatccta aaaaaggcca gacatacagc tgcaagctgt
    12361 ggctggaagg atccaatctc aagcttcgcg gttaccttgg accattctac cgcacacaaa
    12421 cctggtatcg taactaatat caggttcaaa agccgcgcgg cgtaagcccg cggctgattt
    12481 ctaaattttc tcaactaacg gcttgatgtg cgggatctac tcgatcgttt gcaacaagtc
    12541 ctgaaattcg ctcctccggg aattccaatt ctctgcgttt caggtggcca ggattcaatg
    12601 ctgctccttc acgcataccg ctctcttttc gaatcgggca tt
//
LOCUS       DAORFF010000024        12587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2170_length_12587_cov_3.137328, whole genome shotgun sequence.
ACCESSION   DAORFF010000024 DAORFF010000000
VERSION     DAORFF010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12587
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2170_length_12587_cov_3.137328"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PKK76_01965"
     CDS             <1..801
                     /locus_tag="PKK76_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNL67255.1"
                     /translation="ELYSLSSSRISNSKLDPEKIRTAEASVIYYPTETIRTESVIFAS
                     YVRDLISLAATDVHTPGKSQSTYLSAFQNAGSAQIYGTQLSAEGDILPWLRALWNYTY
                     QQSWIFDAKDSNLLAHSGAIDESLRPSDTTICRNTLVSTGYSQFNPLCPRAHGRMPRI
                     ASHKMTAGVTILLSQHWQIDTRLNYVGSRRTIATDPVTSVPSYVLANISLGTKDFPLK
                     GMDITLKVFNAFNEAILDPGTRDGRGDYFPSVHPQPERYLGWDLTYRL"
     gene            974..1258
                     /locus_tag="PKK76_01970"
     CDS             974..1258
                     /locus_tag="PKK76_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNL67256.1"
                     /translation="MTIKPLGDRVLVRPLSQSEEKVGSLFIPDTAKEKPQEGMVEAVG
                     PGRTEDGKTIAVEVKVGDRVLYGKYSGTEIKKDGKEMLIIRESDILAVLS"
     gene            1269..2918
                     /gene="groL"
                     /locus_tag="PKK76_01975"
     CDS             1269..2918
                     /gene="groL"
                     /locus_tag="PKK76_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770238.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNL67257.1"
                     /translation="MAKQLDFHEDARRKLQAGVNKLANAVRVTLGPRGRNVVIDKKFG
                     SPTVTKDGVTVAKEIELEDHFENMGAQMAKEVATKTNDVAGDGTTTATVLAQSIVNEG
                     LKNVTAGANPMHLKRGIDKAVEAVVAEVKKRARKIGTNKDEIAAVASISANNDYEIGK
                     LIADAMAKVGNDGVITVEEAKGIDTTMEVVEGMQFDRGYQSPYFVTDPEAMIATMDKP
                     YILIHEKKIANMRELLPVLEKVAQSGRGLVIISEEVEGEALATLVVNNLRKTIRVVAV
                     KAPGFGDRRKAMLEDIAILTGGQVISEDLGLKLDNADMSQLGQANKVVVDKENTTIIE
                     GAGKDADIKGRIAQIRKQIDDTTSDYDREKLQERLAKLSGGVAVVNVGAATEVEMKEK
                     KARVEDALSATRSAVEEGIVPGGGLTLLRAREVLKELKLEGDEQTGANIVYRALEEPL
                     RQIANNSGQEGSVIVQKAMTEKDNIGYNAATLVWEDLVKAGILDPAKVVRSSLQNAAS
                     IGGMILTTEVAITDLPEKDKGGMPGGMGGMGGMGGMGGMDM"
     gene            3085..6831
                     /locus_tag="PKK76_01980"
     CDS             3085..6831
                     /locus_tag="PKK76_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL67258.1"
                     /translation="MLQAKLQAAIRHVPCERSDGLAAARTRYATSGLSQLFEFDLARN
                     PSIPGSRKNMIPFPTISESPTAWEYFWHQLPWAVTGFLTFVVGVSLAAVGVYAALRRT
                     DKGLLINFVLFSLGYGCLGLVLGFRAVILDRDLLLLLNRIPYFAVVLLSPGGYLFTYY
                     LTDQRYKSLLYAAGAAVVTVVLSIGGLIIGYDFTGEWFHYPFGNFPIAHFPLRLWGLV
                     SALGYLITAVPMTFRYYRDHRADLWEKRYLFIGLHLTSILIISNLPSLAGIPLFPMSS
                     FAFIPLLIMAYGIFHSDFLSVNDLLFQKRGLFKLLAGLVTSGFIIAALATAAEINPTQ
                     HIALYLKPTFLIPLFSGVCAFSLAIFLAGSNPDQKLSMLAAASLMLTGAFMVVMTTIK
                     LDLSPVVTRRIEQIMYTFFVFTPSVHMRFAFHSMKQPSPRFVRLFDIAGVISAIVLWT
                     PYFFSGFYEYSFGRISVGGIGLNLYGFVGVVCAATFLFTWWKTSRKEKTPGGLVIFAL
                     VVGDLLILLNLPATMGIPLYTLGDLQFIPAFILAFAVLNQGAISVEGEATAIGNRISV
                     LILLFVPVSMLFYFISLAEQGVPALAAISHIALVASPVALAFYMISFVFLRPTALKMD
                     ETIRQLAHEKEKTDAALNETERARREIEALSQFTHLINSYTDLDKIFRAISEYVYTQF
                     SISALWLFLPDEKREFLQSYKAYSYNKIPEESYRKLRNLRLPLNENGGIAWAVWERKK
                     SLYLPKIKRYAFGVDREIVALSGATSFLHVPLVVNDRAVGLMAFSNVEENMKLKPREI
                     RTIEAFCAQIAGVVNTAHLLKTTEKQKLDTENLNRFIKSLNEELDMDVIMKKIHEYVK
                     ETFQIQYYALYTLDATRSHFESVQIELPPGVSDQDALSIKKITFPVKKGRGANGYALR
                     SKKPFYTPVVENLLSLATPDEQFVVEKCRVRSMILLPLVLNGEAIGVLNLSNSDEEMN
                     LSQEDITRLSILAEHLAGIVFGSNLFQQVRVAKNQADEERGKSERLLLNILPGDVAAE
                     LKEKGATEPVLYESVSVMFTDFKGFTQIAETMTPRELIQDLDACFVQFDKITERFNLE
                     KLKTIGDSYMCAGGIPKRNTTHAVDCVLAALEIQAFMNLMRDIKENQGLPYWELRLGI
                     HTGPLVAGVIGEKKFAYDVWGDTVNTASRMESSGVPGRINISGTTYEEVKDLFECEYR
                     GLVNAKNKGEVAMYYVLGIKPEFAQDGDPRAPNAKFWQQYATVT"
     gene            6910..7278
                     /locus_tag="PKK76_01985"
     CDS             6910..7278
                     /locus_tag="PKK76_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67259.1"
                     /translation="MKREMVGFRSAILASVFAAHCTGAYGGIDGPSVIARNEAVSRIN
                     DAKLARMAVCGYNSAGAISLYNDYVRLPRKVLDGAYYATRDVDRCVESIWLTPCNQWP
                     LDCGLSPKEIAEPPLLEGSI"
     gene            complement(7288..9984)
                     /locus_tag="PKK76_01990"
     CDS             complement(7288..9984)
                     /locus_tag="PKK76_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNL67260.1"
                     /translation="MLVRGVILLIATATATFAQSIAIGDFESLGGKPEVELASTIRNL
                     LLSQFVAEGFRAELVSVPSSDMPVAQGADLTIVGQYIRTENGLLLYGQIFTERGMIEA
                     YSLETDLPLYRLNDQEMLESDRARIERFGKRVVARVKTNPRSILRADNIAEHFKGTRV
                     GTRPAKIAKADADSQDAFKVFREGISVASNVADRAEKQPAAVTVITRSQVKSSGARTV
                     NELLTTFVPGYFAVEDQDDVIAGFRGLAPDNNSKVLLLINGHNMNAEWFGGPPDSLLN
                     GMSLEYIERIEVIRGPGSVTLGQGALLGVINIVTRNADNAPGVGFYGAGGQNGFGSGS
                     IQAGATGQVIEDLKAFVLFQATNYSGQKLRDEGWAHDRTFEGVEGWYDARVKPGTAST
                     LEIYKDPAQSGSRLKRGNNRIALAELDYAGLAYEGIYTDQLRDYYNFYRDRDEYRNVV
                     FSNDVSYKFQFSPNVFAKARANYATDDSIYQSHGGHIMGGTREARYGGSTILRWNAAH
                     NRNRLGVGGEYRRYDMGQPDVNGNNFIVNKADTTLFDDVNRNHRYVFPSSIIVYSAFA
                     EDFFALTPSLEFFGAVRYDKHPYWGGNLSPRIGLFYDFARNLRTRFSYQEGFRGSTGV
                     SYSGGFQGDGLLRASNYDKVALSRIPTTDQFNQPTYYGNIANLEPEKIRTYELAFNYE
                     PGDRLSFDAVTFYNTIQKVIDVGVIYADPTAFKMPRIGTDVAGDWNGYFFFRNLPGVI
                     HAGGAEASGTYKLPRLSITLSHSVVRVLTVSDSMYFSPYNGGMYLTNKKFNPHFRAFP
                     ENITRAHVSFTPRNWTFGVNYLYYANWYSPVGNRIEGNHILNGNVGYTIGPRMEISLI
                     VKNMLAEGALYPMNSNAGDAAVSDGSPALEGRTYLVQFRYSF"
     gene            10188..10838
                     /locus_tag="PKK76_01995"
     CDS             10188..10838
                     /locus_tag="PKK76_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNL67261.1"
                     /translation="MIGKHWSYEKPEEWGASCNTGRLQSPVDIQKTTHANLESVRIDY
                     SDTPLAITNNGHTIQVNAAGAGGITVGETRFNLVQYHFHARSEHAIQGATADMEVHFV
                     HKDAAGNIAVVGVLLRKGESNPLLKAIVENAPKEKGEKTVSTRGNPALLLPPSRGYYT
                     YVGSLTTPDCSEGLKWIVMKMPVEVSEQEIALFRAMYANNARPVQALNNRIILESE"
     gene            10905..12185
                     /locus_tag="PKK76_02000"
     CDS             10905..12185
                     /locus_tag="PKK76_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67262.1"
                     /translation="MLWIKNHKKTTGLLVALLLLFAVFLRLSAWDDSTRPSADLSQVH
                     PESTPGTLRDVVVVGNNWDGTADVFDPTTFKKLKRFDIVPDFDERMAEVNSSRIRRLF
                     FRLIRNLIGEGHNQLVDDMFTSHDGKFLFVSRPSFADVISVEVQTGKIVWRTKVDGNR
                     ADHAAISPDGKVFLVSASTAKKVQAIDTSTGKIVGEFESGDQPHENNYSKDGKRIFHA
                     SIGKVYLPTTSAIFDRFKGARYFEIVDAQSYKVLKRINMKEKLEESGHPWFDSAIRPM
                     AISPDERFVYLQISFYHGFFEYDLESDKITRVADLPIPEEIQKKNYSGYQLNSAHHGI
                     AINHEGTKLCVAATMSAYAAIVHRDTFKYTIVPVGPKPYWSTESAVGGNCYVSVSEQD
                     RVAVISWDKEKEIASVPVGRHPQRVRTGKMILGR"
     gene            complement(12192..>12587)
                     /locus_tag="PKK76_02005"
     CDS             complement(12192..>12587)
                     /locus_tag="PKK76_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNL67263.1"
                     /translation="RVSFTEVFFGLPLPGVFIDKLKYTVSPSHLNDMVYGETYKAQEA
                     LSAGFVNAIADSREDLRKLTMRKLEALTRIPSSAFIHTKASLLHETVSRIEFHAKATD
                     KNFSAPTIRGNLMEAMKSFTEGRRPKFSD"
BASE COUNT         3306 a         3237 c         3090 g         2954 t
ORIGIN      
        1 gaactgtatt ctctttcttc gagcaggatt tccaatagca aactcgatcc ggaaaaaatc
       61 agaaccgcgg aagcaagcgt catctactat ccaacagaaa caattcgcac agaaagcgtg
      121 atcttcgcaa gctacgtgcg cgatctcatc agcctggcag ccacggacgt tcacacaccg
      181 ggaaaatcac aatctactta cctctccgca tttcaaaacg caggtagcgc gcagatttac
      241 ggcacacagc tttctgcaga aggagacatt cttccctggc ttcgcgcgtt gtggaattat
      301 acctatcaac aatcgtggat ttttgatgcg aaggattcca atctactggc tcactctggc
      361 gcgatcgacg aatcgctcag gccttctgac actacgattt gcagaaacac cctggtctct
      421 accgggtaca gccagttcaa tccactctgc ccgcgcgcgc acggaagaat gccccgcatc
      481 gcttcgcaca aaatgactgc aggagtgacg attctacttt cgcaacactg gcagatcgac
      541 actcgcttga actatgttgg atctcgcaga accattgcaa cagatcctgt gacttctgtt
      601 ccctcctatg ttctggcaaa catcagcctg ggaacaaagg acttccctct caaagggatg
      661 gacatcactc tcaaagtgtt caatgcgttc aatgaagcga tcctcgatcc tggaacgaga
      721 gacggccgcg gggattattt cccgtcggtt catccgcagc cagagcgata tttgggctgg
      781 gatttgacgt atcgtttgtg aggtccctat ttctacctct gaatctggca ctctatgccg
      841 tcgactgcca gatttttacc aaaatcgctt gactgaggac catttttcag tttcttggcc
      901 cttgaattgg cactcgcatg ggtagtctgc taatcaggcc cctggcgagt tggaaatcat
      961 tcaggaggaa tgcatgacaa tcaagcctct cggagaccgg gtactggttc gtccattgtc
     1021 ccagtctgag gaaaaagttg gatccctatt tattccagat acggcgaaag aaaagccgca
     1081 agaaggaatg gtggaagccg taggtccagg ccgtacagaa gacggcaaga ccatcgcggt
     1141 agaagtcaaa gtaggcgatc gtgtactgta cggaaaatac tccggcacag aaatcaaaaa
     1201 agacggcaaa gaaatgctga tcatccgcga gagcgacatt ctcgcagtac tgagctgagg
     1261 aggaaaccat ggcgaaacaa ctcgactttc acgaagacgc gcgccgcaaa ctgcaggcgg
     1321 gcgtaaacaa actcgcaaac gcagttcgtg taacgcttgg gccacgcgga cgcaatgtgg
     1381 tcatcgataa gaaatttggt tcaccaactg tgaccaaaga cggtgtaact gtcgcaaaag
     1441 aaatcgaact cgaagatcat tttgagaaca tgggcgcgca aatggccaaa gaagtggcca
     1501 cgaaaacaaa cgacgttgcc ggcgacggaa ccacaacagc aactgttctc gcgcaatcca
     1561 tcgttaacga aggactcaag aacgtaactg caggcgctaa cccaatgcac ctgaaacgcg
     1621 gaatcgacaa agctgttgaa gcagtagtcg ctgaagtgaa aaagcgcgca cgcaagatcg
     1681 gcacaaacaa agacgaaatt gcagctgttg cttctatctc tgcaaacaac gactacgaaa
     1741 ttggcaaact catcgcagat gcaatggcaa aagttggaaa cgacggcgtc atcactgtag
     1801 aagaagccaa aggcattgac acaactatgg aagtagttga aggcatgcag ttcgatcgcg
     1861 gctaccagtc tccttacttc gtaacagatc cggaagcaat gatcgctact atggacaagc
     1921 catacattct cattcacgag aagaaaatcg caaacatgcg cgaacttctt cctgtgctcg
     1981 agaaagttgc acagtccggc cgcggtctcg tcatcatttc tgaagaagtg gaaggcgaag
     2041 ctctcgcaac tctcgttgta aacaatctgc gcaagaccat ccgcgtggta gctgtaaaag
     2101 ctccaggctt tggcgatcgc agaaaagcaa tgctcgaaga catcgcgatc ctgactggcg
     2161 gtcaggtgat ttctgaagac ctcggtctga aactagacaa cgcagacatg agccagcttg
     2221 gccaggcaaa caaagtggtt gtagacaaag aaaacacaac catcatcgaa ggcgcaggca
     2281 aagacgcgga catcaaaggc cgcatcgctc aaatcagaaa gcaaatcgac gacactacat
     2341 ctgactacga ccgcgaaaaa ctccaggaac gcctggcaaa actttcaggc ggcgttgctg
     2401 ttgtaaacgt tggtgctgca actgaagtcg aaatgaaaga gaagaaagct cgtgtggaag
     2461 atgcactttc tgctactcgc tctgcggtag aagaaggcat tgtgccaggc ggcggactca
     2521 ctcttcttcg cgcgcgggaa gtgctcaaag aactcaagct cgaaggcgat gagcaaactg
     2581 gagcgaacat cgtatatcgc gctctagaag aacctcttcg ccagatcgca aacaactccg
     2641 gccaggaagg atcggtgatt gtccagaaag caatgactga gaaggacaac attggataca
     2701 acgccgcaac tctggtatgg gaagacctcg tgaaagcggg cattctcgat ccagcgaaag
     2761 tggttcgcag ctctctccag aacgctgctt ccattggcgg tatgatcctc acaaccgaag
     2821 ttgccatcac tgacctccca gaaaaagaca aaggcggaat gcctggtgga atgggcggca
     2881 tgggtggtat gggcggaatg ggcggaatgg acatgtaatt cgcgtcccaa acgcgcaaac
     2941 acaaatcaaa gaaagccccg actcgcttcg gtcccgcaag caggccctcc atggcccggt
     3001 tgcgggacaa ccctccctcg tggagggttt acgaagcgag tcggggcttt cctcttttac
     3061 gatcaaggtt tttgtgccgc aaacatgttg caggcaaaac tgcaggctgc tattcgccac
     3121 gttccgtgtg agcgaagtga tggactagcg gcggcgcgaa cacgttatgc gacgtccggc
     3181 ctctcgcaac tcttcgagtt tgacctggca cgcaatccat ccatacctgg ctcccgaaag
     3241 aacatgattc ccttccctac catttccgaa tcgcccactg cgtgggaata tttctggcac
     3301 cagctaccgt gggccgtcac tggatttcta acgttcgtcg ttggcgtgtc tcttgccgcg
     3361 gtcggcgtgt atgcagctct tcgcagaaca gacaaaggcc tgctgatcaa ttttgttctc
     3421 ttctctctgg gttacggatg cctgggactg gttctgggtt ttcgcgcggt gattctggat
     3481 cgcgatctct tactactgct caatagaatt ccgtactttg ccgttgtgct tctctcgccg
     3541 ggaggctacc tcttcaccta ctacctgaca gaccagagat ataaatctct tctctatgct
     3601 gcaggtgcgg ctgtagtcac agtcgtgctg agcattggcg gattgatcat tgggtatgat
     3661 ttcacgggag agtggttcca ctatccgttt ggaaattttc ccatcgcgca cttccctctg
     3721 cgtttatggg gcctggtttc cgcacttggt tacctgatca ctgctgtgcc tatgaccttt
     3781 cgctattaca gagatcatcg tgcggacctc tgggaaaaac gctacctgtt catcggattg
     3841 catctaacgt ctatcttgat catttccaat cttccgagcc tcgctggaat tccactcttc
     3901 cctatgagca gcttcgcgtt tattccactg ttgatcatgg cttacggaat ctttcactct
     3961 gattttctga gcgtgaacga tcttcttttt caaaaaagag gactcttcaa acttctggcg
     4021 ggactggtca cgagtggatt tatcatcgcg gccctcgcga ccgcagcaga gatcaatcca
     4081 acacaacaca ttgcgctcta cttaaagcca acgttcctca ttccattgtt ctctggagtt
     4141 tgcgcattca gtctggctat ttttctggcg ggaagcaatc cagaccagaa gctgagcatg
     4201 ctcgccgctg cttccttgat gctcacggga gcattcatgg ttgtgatgac taccatcaaa
     4261 ctcgacctgt ctcctgttgt tacgcgcaga atcgaacaga tcatgtacac attctttgtt
     4321 tttactccca gtgtgcacat gcgcttcgca tttcattcca tgaagcaacc cagcccacga
     4381 ttcgtaagac tgtttgatat tgccggcgtg atttctgcca tcgtgctgtg gacgccctac
     4441 ttcttttccg gtttctatga atattctttt ggaagaatct cagtaggtgg aattggcctg
     4501 aatctgtatg gatttgttgg agttgtttgc gccgccacct ttctgttcac ctggtggaaa
     4561 accagtcgca aagaaaaaac accgggcggc ctggtcatat tcgctcttgt cgtcggagac
     4621 ctactcattc ttctcaacct cccggccact atgggtattc cactctatac gttaggcgat
     4681 cttcaattca taccagcctt catcctggcc ttcgcagtgt tgaaccaggg agccatttca
     4741 gtagaggggg aagcaacggc gatcgggaat cgaatctctg ttttgattct cctgttcgtg
     4801 ccggtgagta tgttatttta tttcatttct ctggcagagc aaggagtgcc cgctctggcc
     4861 gctatttcgc acattgcact ggttgcttct ccagttgccc ttgcattcta catgatttct
     4921 ttcgtgtttt tgcgaccaac tgcactcaag atggatgaaa cgatccgaca gctcgcgcat
     4981 gaaaaagaaa aaacagacgc ggctctcaat gaaacggaaa gagccaggcg agaaatagag
     5041 gcactcagtc agtttacgca tttgatcaat tcatacacgg atctggataa gatatttaga
     5101 gccatctcag aatacgtgta cactcaattc tccatctccg cgctctggct tttcttgccg
     5161 gatgaaaaaa gagaattctt acaaagctac aaggcataca gttacaacaa aattcccgag
     5221 gaaagctacc gcaagcttcg caaccttcgc ctcccgttga atgaaaatgg ggggattgcg
     5281 tgggcagtgt gggagagaaa gaaatcactc tatcttccaa agatcaagag gtatgccttt
     5341 ggcgtcgaca gggagatcgt tgcgctttcc ggtgctactt ccttcctgca tgtgccgctt
     5401 gtagtgaatg atcgggccgt gggcctcatg gccttttcga acgtggaaga gaatatgaaa
     5461 ctcaaaccac gggagattcg aaccatcgaa gctttttgcg cgcagattgc aggagtggtc
     5521 aataccgcac acctcctcaa gacgacggaa aaacaaaaac tggatacaga aaatctgaat
     5581 cgcttcatca agagcctcaa tgaggagcta gacatggacg tcatcatgaa gaagattcat
     5641 gaatacgtca aagagacctt tcaaatccag tactatgccc tctacactct ggatgcaacg
     5701 cgcagccact tcgagtctgt tcagattgaa ctgccacctg gagtgagtga ccaggatgcg
     5761 ctcagcatca agaagatcac atttccagtt aagaaagggc gaggcgcgaa tggctacgct
     5821 ctgcgaagta agaaaccatt ttacacgcca gtcgttgaga acctgctctc cctggccacg
     5881 cctgatgaac aatttgtagt agagaaatgc agggttcgtt ccatgatcct gttgcctcta
     5941 gtcctgaacg gggaagccat cggggtactg aatctctcca actccgacga agaaatgaac
     6001 ttgagtcagg aagacatcac tcgactgtcc attctggccg aacacctggc aggaattgta
     6061 ttcggatcga acctgtttca gcaggtgcga gtggccaaaa atcaagcgga cgaagagcgc
     6121 ggcaaatctg aacgactgct actaaacatt ctgcctggcg atgttgcggc ggaactgaaa
     6181 gagaaaggtg ccacagaacc ggtgctatac gaaagcgtca gtgtgatgtt cacggacttc
     6241 aaaggattta cgcaaatcgc agaaaccatg actccgcgcg aattgattca ggatctggat
     6301 gcatgcttcg tgcaattcga caagatcaca gaacgattca acctcgagaa actaaaaaca
     6361 atcggagaca gctacatgtg tgcgggtggg attccaaaaa gaaacacaac gcacgccgtg
     6421 gactgtgttc tggccgctct ggaaatccag gctttcatga atctgatgcg cgatatcaag
     6481 gaaaatcagg gccttcccta ctgggaactc cgtctcggaa tccacacggg tccgcttgtg
     6541 gccggtgtaa tcggagagaa aaaattcgct tatgatgtgt ggggcgacac ggtcaatact
     6601 gcatcgagaa tggagtcatc tggagtacct ggcaggatca atatcagcgg cacaacctac
     6661 gaagaggtca aagacctttt tgaatgcgaa taccgcggcc tggtcaatgc gaaaaacaaa
     6721 ggcgaagtgg caatgtacta cgttctcggg atcaaaccag aattcgcgca ggacggagat
     6781 ccaagagcgc ctaacgcaaa attctggcaa caatacgcta cagtcaccta gcacaaacgg
     6841 ttccgcagaa cagttttttg aattgcgcaa agcaaacact gagttcatac agcagaagtg
     6901 ggagcatgga tgaaaaggga aatggttgga tttcgatctg ccattctggc gtctgtgttc
     6961 gccgctcatt gtacgggcgc ctacggcgga atagacggac cttctgtcat tgctcgcaat
     7021 gaggcagtct cacgaatcaa cgatgcgaag ctggccagaa tggcagtctg cggctacaat
     7081 tctgcgggtg cgatttctct ctacaatgac tacgttcgtc ttcctcgaaa ggttctggat
     7141 ggcgcctact atgccacaag ggatgtggat cggtgtgtgg agagcatctg gcttactccg
     7201 tgcaaccagt ggccactcga ttgcggactt tcccccaagg aaatcgcgga gccacctttg
     7261 ctcgagggca gcatatgatg ctgctcctca aaaagaatat ctaaattgaa ccagatacgt
     7321 gcgtccttcc agagccgggg aaccatctga aacagctgca tccccagcgt tgctgttcat
     7381 cggatagaga gctccttctg cgagcatatt cttgacgatc aaactgattt ccattctggg
     7441 gccgatcgta taaccaacgt ttccgttcag aatgtgattt ccctctatgc gatttccaac
     7501 gggcgaatac cagtttgcat agtacaagta gttcactcca aacgtccagt ttcgcggtgt
     7561 aaaggaaaca tgtgcacgcg ttatattctc cggaaacgcg cgaaagtgag ggttgaattt
     7621 cttgtttgta agatacatcc cgccattgta cggactaaaa tacatactat ccgaaacagt
     7681 cagaacacgc acgactgaat gcgaaagagt gatagataac cggggaagct tgtaggtccc
     7741 gcttgcctcc gcacccccgg catggattac accaggtaga tttctgaaaa agaaataacc
     7801 attccagtct cccgccacgt ccgtgcctat gcgaggcatt ttaaatgcag tcggatctgc
     7861 atagataaca cctacatcaa tcaccttttg aattgtgttg tagaacgtta ccgcgtcaaa
     7921 ggaaaggcga tcccctggtt cgtagttaaa tgcaagctcg taagtacgga tcttttctgg
     7981 ctccagattc gcaatgttgc cgtagtaagt gggctgattg aactgatccg ttgtgggaat
     8041 tctggacagg gccaccttat cgtagttaga cgcccgcagt aggccatccc cctggaaacc
     8101 tcccgaatac gatacccctg tagatccccg gaacccttcc tgataagaaa aacgcgtacg
     8161 cagattgcgg gcaaaatcgt agaatagacc aatcctggga gaaagatttc caccccagta
     8221 tggatgttta tcgtatctca cagcaccgaa aaactccaga gaaggagtga gagcaaagaa
     8281 atcctccgcg aatgcggaat acacgatgat cgaggaagga aaaacgtatc tgtgatttct
     8341 atttacatcg tcgaataacg ttgtgtcagc cttgtttacg atgaagttat tgccgtttac
     8401 atccggctgg cccatgtcat atcgcctgta ctcccctccc acacccagac gattcctgtt
     8461 gtgagcggca ttccagcgaa ggatcgtgga accaccgtat cgcgcctcac gcgtccctcc
     8521 catgatgtga ccgccatggc tctggtaaat actatcgtct gttgcgtaat tggctctggc
     8581 cttcgcaaat acgttaggtg aaaactggaa cttgtaggag acatcgtttg aaaaaacaac
     8641 attccgatac tcatcacgat cgcgatagaa gttgtaatag tcacgcagtt gatctgtata
     8701 aattccctca taggccagac ctgcgtagtc caattccgca agcgcaatcc gattatttcc
     8761 gcgcttgagt ctacttccgc tttgtgcagg atccttgtat atttccagag tacttgccgt
     8821 tcccggcttc actcgagcat cgtaccagcc ctctacacct tcaaaagttc tatcgtgcgc
     8881 ccagccttcg tcgcggagtt tctgtcccga gtagttcgta gcctgaaaga gaacgaacgc
     8941 tttgagatct tctatcacct ggcccgtggc acctgcctga atcgatccag aaccaaagcc
     9001 attttgacca ccagccccat aaaaaccgac accgggtgca ttgtccgcgt ttcgcgtaac
     9061 aatgttgatc acaccaagca aagcgccctg accgagagtg actgaaccgg gcccgcgaat
     9121 cacttctatc ctctcgatgt actcgagact catgccgttg agaagagagt caggcggtcc
     9181 accgaaccat tccgcattca tgttgtgacc attgatcagc agaagcacct tcgagttgtt
     9241 atccggtgca aggccgcgaa aaccagcaat cacatcgtcc tgatcttcca ctgcaaaata
     9301 gccggggaca aacgtggtca acaactcgtt gactgtacgt gcgccgcttg actttacctg
     9361 agatcgcgtg atgacagtca cagccgcggg ctgcttctcc gccctgtcag cgacgttaga
     9421 cgccacagag atgccttcgc gaaatacttt gaaagcgtcc tgcgaatccg catccgcctt
     9481 cgcaatcttc gcgggcctgg tccctacccg cgttccttta aagtgctctg cgatgttatc
     9541 tgcacgcaaa atgcttcggg gatttgtttt cacgcgcgcc accactcgtt tcccaaagcg
     9601 ctcaatcctc gccctgtctg attcaagcat ttcctggtca ttgaggcgat agagtggtaa
     9661 atcagtttcg agactgtagg cctctatcat gccgcgttct gtaaagatct gaccatacaa
     9721 aagaagaccg ttttctgttc tgatgtactg gcccacgatc gttagatccg cgccctgggc
     9781 cacgggcata tctgaggaag gaacggacac aagctctgct cgaaacccct cggctacaaa
     9841 ctggcttagt aaaagattgc gaatggtgct cgccagctcc acctctggct tccctccgag
     9901 cgattcaaaa tcgccgattg cgatcgattg tgcaaaagtt gcagtggcag tcgcaatgag
     9961 cagaatgact ccgcgaacga gcatacccga aggataggag cacggacact gagtccgggc
    10021 aaggtctttt ttacaattgt catgaaatct ggcggaagac agaaccgaac cgtgcccaga
    10081 ggctcgcgaa aaggtttcca gaatcgcccg gatggttttt caatggctgg atgagactga
    10141 ttgtgatcgc agtattgtcc ctattgtcat gccagaaatg gaatgagatg atcgggaaac
    10201 actggtcata cgaaaagcct gaagaatggg gggcaagctg caatacaggc agactgcaat
    10261 cccccgtgga catccagaaa accacgcacg caaatctaga aagtgtaagg atagactact
    10321 cagatacacc gctcgctatc acgaataacg gccacacaat ccaggtaaat gcggccgggg
    10381 ccggcgggat caccgtggga gaaaccagat tcaatcttgt gcaataccac tttcatgccc
    10441 gcagtgaaca cgccattcag ggagccaccg cagacatgga agtgcatttc gttcacaaag
    10501 atgcggcggg caacattgcg gtggtaggag ttcttctaag aaaaggagag agtaaccccc
    10561 tgctcaaagc aattgtggaa aacgccccaa aggagaaggg cgaaaagaca gtatctaccc
    10621 gcggaaaccc ggcgcttctc ctcccgcctt cgcgcggtta ttacacgtac gttggctccc
    10681 tgaccactcc cgattgctca gagggattga agtggattgt aatgaagatg cccgtagaag
    10741 tatccgagca ggaaatagct ctctttcggg ccatgtatgc gaataacgct cgtccggtgc
    10801 aggctctgaa caatcgcatt atactcgaaa gcgaatgatg gaatcgggaa aaatgctttt
    10861 ctgaccaaag ggtgagtcca gcttcaggcg ggggatcaaa caccatgctc tggatcaaga
    10921 atcacaaaaa gaccaccggc cttctcgtcg cattactgct attattcgca gtcttcttac
    10981 gcctgtctgc ctgggacgat tccactcgcc cttccgccga tctaagccag gtccatccag
    11041 agtccacacc gggcactctc agggatgtag tagtagtcgg gaacaactgg gatggcacag
    11101 cagatgtttt tgatccaacc acattcaaga aattgaaacg atttgacatc gttccggatt
    11161 tcgacgagcg catggcagag gtaaactcca gcaggatccg aagattgttc tttcgcctca
    11221 ttcgcaacct cattggggaa ggacacaacc agctggtgga cgacatgttc acttcacacg
    11281 acggcaaatt cctcttcgtt tctcgtccca gtttcgcaga cgtgatctct gtggaagtgc
    11341 agacgggcaa aattgtctgg agaacgaaag tagacggaaa tcgcgcggac catgcagcta
    11401 tttcgcctga cggcaaagtt ttccttgttt ctgcttccac tgcaaagaaa gtccaggcaa
    11461 ttgatacgag cacgggcaag atcgtgggtg aattcgaatc gggagatcaa ccgcacgaaa
    11521 acaactattc gaaagacggc aaacgcatct ttcatgctag cattggcaaa gtatatctac
    11581 ccacaacgtc tgcgattttt gatcgattca aaggcgcgcg ctatttcgaa atcgtagacg
    11641 cgcaatccta caaagttctc aagcgaatca acatgaaaga gaaactggag gaatccggtc
    11701 acccctggtt tgattctgcc attcgcccga tggctatttc ccccgatgaa agatttgttt
    11761 atcttcaaat ttccttttac cacggattct ttgaatacga tcttgaatct gacaaaatca
    11821 ctcgcgtcgc agaccttccc ataccggaag aaattcaaaa aaagaattat agcggctatc
    11881 agctcaattc agcgcaccat ggaattgcga tcaatcacga aggaacaaaa ctttgtgttg
    11941 cagcaaccat gtccgcgtat gctgccattg ttcatcgcga tacgttcaaa tacacaatcg
    12001 ttcctgtggg tccaaagcct tactggtcta ctgaaagtgc tgtgggtgga aactgctatg
    12061 tttccgtaag cgaacaggac cgcgttgcag tcatctcctg ggacaaggaa aaggaaattg
    12121 caagcgttcc cgtagggcgc cacccgcagc gcgttcgcac aggcaaaatg attctgggtc
    12181 gttgagggaa ctcaatctga gaatttcggg cgacgaccct ctgtgaacga tttcatggcc
    12241 tccatgagat tgcctcgaat cgtcggggcg gagaaatttt tgtctgttgc cttcgcatga
    12301 aattctattc tggaaacggt ctcatgcaac aaggaagcct ttgtgtgaat gaacgcagag
    12361 gacgggattc gagtcagtgc ttctagcttg cgcatggtaa gcttgcgaag gtcttcgcgc
    12421 gaatctgcaa ttgcgttcac aaatccggca gagagtgctt cctgtgcttt gtacgtttct
    12481 ccgtatacca tgtcgttgag atgggaagga gaaactgtat acttgagctt atcaatgaac
    12541 acaccgggaa gtgggagtcc aaagaacacc tctgtaaagg agacacg
//
LOCUS       DAORFF010000025        12411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2228_length_12411_cov_3.864276, whole genome shotgun sequence.
ACCESSION   DAORFF010000025 DAORFF010000000
VERSION     DAORFF010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12411
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2228_length_12411_cov_3.864276"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="PKK76_02010"
     CDS             <1..1075
                     /locus_tag="PKK76_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67264.1"
                     /translation="LEMYRTYSQYPHDSRPLTASMKDLTNPWFVEQVPLPIPAPGAAN
                     EQEIKELVEKMQASGKSNDDIEKEIAKMIENQPAFQFVLNRHTVTEGDTLVATLKITK
                     NGSPIPYTISSAELESDPSQGLKGLGSISFNDNGVAPDEKAGDGITTFSWKAPSADKK
                     YWGTLTIKAKVNVRGAKDVAVVQQSFYSSPSAPAVFQNSFSERLQDGSLVIDIPIEVR
                     RECRYSLQANLYSIDENEPTHWAVVQKVLDPGRQIVSFTFFGKIFHDGGYSGKFQIRD
                     IRGTCENMPYPAKWLGDPSKANQIANTPAVNEPPIIYIPFNDYAYTTRSYNLDEFSNQ
                     EYTSAEKTKRLAELEEAAKASRE"
     gene            1072..1668
                     /locus_tag="PKK76_02015"
     CDS             1072..1668
                     /locus_tag="PKK76_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67265.1"
                     /translation="MIYFFRTPFMFFPRLVTISVLLLMVGCHSRTEMKSEVRCADGSG
                     IEMSVTYLENRRADPIAGSTDKSDYVTAVRRSGREAKIQGWLLSEGAHCGKTAFFLGG
                     EGDQFGVNPQLFSWDGARIKRVSESGPVEKFAVSLDERYAALMFLREKIVFVEIQTGK
                     TVAEEKISGTFDRLDWSGKKLLLSGPSGDLSILPRLAQ"
     gene            complement(1643..2554)
                     /locus_tag="PKK76_02020"
     CDS             complement(1643..2554)
                     /locus_tag="PKK76_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNL67266.1"
                     /translation="MSSHQESSFRAILYAFLANFGIAAGKSVAAYLTGSGSMLAEAVH
                     SFADCGNQLLLFLGLHRAQMPPTRNHPLGFGKVVYFWSFLVAILLFTLGGIFSIREGI
                     HKLTTPEPLAHVPIALGVLGFSVILESFSMYGCIREIKKIKGTKTLLAWLKGSRNAEF
                     IVVLGEDTAALLGLAVAFTFVTLSYVTGNPIYDAVGSLVIGCVLVVIAFLISQRVKSL
                     MVGMSADPDLENRIEALIEKDKSIVKVFNIITQQFGPHLMLAAKIEMKPSLKISEAAV
                     KINELEKNLKKEFPEIRWCFIEPDVAK"
     gene            complement(2621..3001)
                     /locus_tag="PKK76_02025"
     CDS             complement(2621..3001)
                     /locus_tag="PKK76_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67267.1"
                     /translation="MLSIIHAAICSGPVFFLIVSYFVGPKNAPQSFLAQIGWIFGAGS
                     AAASFFIPRLLAGGKEEINAGSEPSDSQGNLNFVQKIVQWAIVEAGALFNGVVYFSTG
                     DSGSAAVSVAMILLLIYFRPTASD"
     gene            3107..4393
                     /locus_tag="PKK76_02030"
     CDS             3107..4393
                     /locus_tag="PKK76_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002105495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HNL67268.1"
                     /translation="MNTPYILGGAQTDFSRNWTKEGKTFMSMMREVVQDGLSQIGLDY
                     DEITRLNKENKIAVFVGNFDAEQYTQQGHLGAFLTEVHPAFYGVPGARYEAACASGSV
                     ALDAASARIRGGDYEVAVVLGIEIMKTVSSSVGGDFLGTAAYYEREAKGVQFPFPKLF
                     GKLADVIVERAKGVGEKRIMDALAEISRINYANAKLNPLAQTRTWFMSEEHAKDRGSQ
                     YNMAVGGRLCIADCSQVTDGAAITVVASPAYAKQFAQKKGIPMSKLAVIKGWGHRVAP
                     VTFDAKVAESKDNEYILPWTRQTVKDAYTRAGIDVDQIDVIETHDCFTSSEYAAISAF
                     GMTKPGEEYQAIEDGRIALDGPAKAGKKPINPSGGLIGAGHPVGASGVRMAVDLFRQV
                     TDQAGDYQVAGAKNGIMLNIGGSATTNMTFVISRAE"
     gene            4485..5705
                     /locus_tag="PKK76_02035"
     CDS             4485..5705
                     /locus_tag="PKK76_02035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNL67269.1"
                     /translation="MKQGSLPVMAILDIIKTLQFPLPFEIHLPDGSHHKHGSGEPVFV
                     MRIKTDAALSDVMRQGSLGFGEAYMREEIDIEGDVQEVSRLGYALMETDALTPTFMEK
                     LKFLFVYFSRRNTREGSRKNIQAHYDLGNDFYSLWLDKEMQYTCAYFHDFKDSLEKAQ
                     LQKLEHVCRKVRLKKGDTVVEAGCGWGGFARYAAKHYGVKIRSYNISQEQIEYAREMQ
                     KKLKIPQSQVEYVLDDYRSIGSSGEKYDKFVSVGMLEHVGRENYYGLYEIIQKVVEKR
                     GMAIVHSIGRRFPRKPDPWLEKYIFPGCYIPSLSDMITPFESLPGHDLHVVDVENLRY
                     HYALTLDHWWMRLEKNAEKIRKQYGEAFVRMFRLYLRAAAAGFRYDELTLLQVVLSNG
                     SDNTAPLTRKHLYK"
     gene            5702..6871
                     /locus_tag="PKK76_02040"
     CDS             5702..6871
                     /locus_tag="PKK76_02040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNL67270.1"
                     /translation="MSNQYDVIVVGAGPGGSNAARHFAKQGWKVLMLDRQKFPRVKIC
                     AGWITPPALRLLDLKPSDYPHTLQRFSSGSVVLGDRYYETDYGTTASYGIIRQEFDTF
                     LARRAQASGVEFLEEVRIKSIERGDSPDALVRVRSEDGTEWTTKLIIGAGGTGCPVAR
                     QWGEKSKEEIIVQATESETKLGADLLRTLTPYYGTTELFPEPDFFGYAWFVTKGDWVN
                     IGIGRFSHRTKQLDTHRDQFMEQLRKLGRLKGIEDKIVPFGRHAYKLYDEVPRKLFGD
                     RFMLIGDAAGFASKWAGEGIRPAIETGEFAARVGNAALKAQNLSANSLSPYKQLCDHA
                     YGPQKDTTMGRLISGLPGSVQTGVGTVICKSDYLRKKLIFEAAFGFEPVRDPVYL"
     gene            complement(6868..7992)
                     /locus_tag="PKK76_02045"
     CDS             complement(6868..7992)
                     /locus_tag="PKK76_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67271.1"
                     /translation="MLHKLAAIILVTASIALASPVHAQAFCQGTGCGAIPFSQDSLNS
                     LFFQFQYQYTNQLFSDIARAGTLANISSAPTGSVNLQKFTVGASGSAGFVPLQNKTLT
                     MPGVGVFQDVPSGGAYAIPHIFGGVNLGYLVGNEYDPYDSNGSTPSMLSLSRVDVYAT
                     GAEYREHYSRDNHNLKVVAGFYNRGFDVRYHLVEGANIAGGPLFRFLGVSLGTGYHLS
                     RLNIQAEQYQKYRVASLSGVQVFWDGYNYLSIQTKIKTYPVEIMSGMQLLYVLSLSVG
                     GGVTTNSGYADMNLTRIGPVYPSTDILNNLLSSAGVNLISNTFTSYLTMNISGRGTIP
                     KHVPYAKVSMDINLGPAKIFAEGISTRRAYGANAGVRVQF"
     gene            complement(8018..8755)
                     /locus_tag="PKK76_02050"
     CDS             complement(8018..8755)
                     /locus_tag="PKK76_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67272.1"
                     /translation="MKILKLLAMGTLLASLTNCFSIMHFLDLQENGTLNVQWSFRFSK
                     ALEDMGKKGDKAESGGLSQEVEKGKQELPGKLGSIAKDLKVEALQSDYDSGIRMSFAV
                     PQYLKLDFSKFQEAEFPYMPRYDAAKKQMIFHFTPAKKDKKEEKPAETKMPDAPKEGS
                     EETSEPATQGGDDMGKQIGKLFLSSVRYQIVLGKNLKVKSVSVSQDGVEKKLELQTIG
                     EQSMIDLPLFALYGDKELPFDLIVQLK"
     gene            complement(8810..10507)
                     /locus_tag="PKK76_02055"
     CDS             complement(8810..10507)
                     /locus_tag="PKK76_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNL67273.1"
                     /translation="MIYTEVKALNTRRENLRWNAWGSLDNDFFYADRIGPILNYIRHT
                     LKMQETITPSLRLTDLRPAESKIKGTNLSSLQRIFGKNRVKTDNAERILHSAGRSYFD
                     VMRLRGNLLKTYVDAVVYPETELEIEKLLKLAVQRNWAIIPFGGGSSVVGGVEAKSGG
                     KKAIVCVDMTRMNRLIALNPVSSVATFEAGIYGPDLELALAKQGYTLGHFPQSFEYST
                     LGGWVAARSAGQQSGRYGKIENMVAAIRMVTPGGLFTTGHYPAASTGPDLNQILCGSE
                     GTLGIITAVAVKLHELPSERTYGGFLFPDFATGLEFIREANQLELSFSMLRLSDEGET
                     SQLESFGRIVSHVQNPGAGERIKRFAKDTIEGIVLQSYGLSERKCLIVAGADGPDSKE
                     AIARLKKLAPRFRGMYAGASPGKKWLKGRFNMPFLRNYLLEYGIGVDTMETAVPYGRA
                     MKVHDEVLAAIQESIPGAIGICHASHSYIDGVCLYFTVMFPMDLDHPVEQWTQLKTAV
                     SDAMLANGGNISHHHGIGNDHRDHFRRQVGPAALATLQSLKKTLDPRGNLNPGKLMN"
     gene            complement(10504..11310)
                     /locus_tag="PKK76_02060"
     CDS             complement(10504..11310)
                     /locus_tag="PKK76_02060"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNL67274.1"
                     /translation="MSSVLEAIERLNRLEKLVLPDLSQALVRIQSGNLGIKTKSNDKD
                     LVTVADVESENRLTSFIREHWPEDSILAEETGGSIGRDLTWIIDPVDGTVNYAHGLPL
                     YSVSVGIALDGKPLAGIVHMPSLGTTYKGAVGHGATKNGNPIRVSETNAASQALVVTG
                     FPYDRATILEALIAGVESVLKNCRGIRRTGSASIDLCWLAEGSFDAHYECNLSPWDTC
                     ASEVILQEAGGRITTFAGEEHRLTDKTLLATNHLLHEAMLQIIAPLRSGL"
     gene            11608..12318
                     /locus_tag="PKK76_02065"
     CDS             11608..12318
                     /locus_tag="PKK76_02065"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNL67275.1"
                     /translation="MDELNIQIILIRHGETEYNREGRIQGTLDSPLTERGRLESSTLG
                     VSMRDWLPPVHQWLVSPQGRARESSRLIRDHFANGGQAVRVLPAEELEESAREINCGD
                     YEGKITETLDQSIIQKVRELADFPYPQGESLVDVMNRGKFVLARVIESSQRAHNAHPD
                     LNEYRVVLVSHGNFIRAFGALLTRLGPEFALRAFQSNTGVNRLVSRDGGRGFKILTWN
                     DTSHMNQHPTEAANLGTA"
BASE COUNT         3127 a         3012 c         3226 g         3046 t
ORIGIN      
        1 attggaaatg tatcgcacgt actctcagta tcctcacgat tcgcgccctc tcactgcatc
       61 catgaaagat cttaccaatc cctggtttgt agaacaagtg ccccttccta ttccggcgcc
      121 aggtgccgcc aacgagcaag agatcaaaga acttgtagag aaaatgcaag cgtccggcaa
      181 aagcaacgac gacatagaaa aggaaattgc gaagatgata gagaatcagc ccgctttcca
      241 gttcgtgctg aatcgccata cggtcaccga aggagatact ctggtggcca cgttgaaaat
      301 tacaaagaac ggttctccca ttccttacac gatttcgagc gcggaactcg aaagtgatcc
      361 aagccagggt ctcaagggcc tgggcagtat ttccttcaat gacaatggag tagcacctga
      421 cgaaaaggcg ggagatggaa tcactacttt ttcctggaag gcgccgtctg cagacaagaa
      481 atattgggga acactcacca tcaaagcaaa ggtgaacgta agaggagcaa aagacgttgc
      541 agtcgttcaa cagagtttct actccagtcc ttctgctccc gctgtgtttc agaatagctt
      601 ttctgagagg ctacaggatg gatcgctcgt cattgatatt ccaatcgaag tgcgtcgcga
      661 atgcagatac agtctgcaag cgaatctcta ctccatagac gaaaatgaac caacgcactg
      721 ggccgtagtt caaaaggttc tagatccagg cagacagatt gtttctttta cgtttttcgg
      781 aaagatcttt cacgacggcg gttatagcgg taagtttcaa atacgcgaca tccgtggcac
      841 atgcgaaaat atgccatacc cggcaaagtg gctcggggat ccgtccaaag caaatcagat
      901 tgcaaacacg ccggcagtca atgagcctcc catcatttac atccctttca acgactacgc
      961 ctacaccact cgctcttata acctggacga attctcaaat caggaataca cgtccgcaga
     1021 gaagacaaaa aggttagcag aactagaaga agccgcgaaa gcgagtcgcg aatgatttat
     1081 ttttttcgca cacctttcat gtttttcccc aggctcgtga caatctcagt cttgcttctg
     1141 atggtcggtt gccattcgcg tactgaaatg aagagcgaag tgcgttgcgc agacggatct
     1201 ggaatcgaaa tgagtgtcac gtatctagaa aacaggcggg cggatccaat cgcaggcagt
     1261 acggataagt cagactatgt aacagcggtt cgtcgatctg gaagagaggc gaagattcag
     1321 ggatggttgc tttcagaggg cgcgcattgc ggaaagactg ccttctttct gggtggagaa
     1381 ggagatcaat ttggagtcaa tccgcagttg ttttcctggg acggggcccg aatcaagcga
     1441 gtttcagaaa gcgggcccgt ggaaaaattc gcggtgtctt tggatgaacg ttatgcggca
     1501 ttgatgtttc tcagggagaa gattgttttt gttgagattc aaacggggaa gactgtggcc
     1561 gaagaaaaaa tcagcggaac ctttgacagg ctggactgga gcggaaagaa actccttctt
     1621 tccggcccgt ccggagacct gtctattttg ccacgtctgg ctcaatgaaa caccagcgga
     1681 tttccgggaa ttccttcttc agattctttt cgagttcgtt gatcttcacc gcagcctcgc
     1741 tgatttttag ggatggtttc atctctattt ttgcagcgag catcaggtga ggaccgaatt
     1801 gctgcgtgat gatattgaat actttgacaa tggacttgtc tttttcgatc aaagcttcta
     1861 ttctgttttc tagatctgga tcagcggaca ttcccaccat gagcgatttc actctctgag
     1921 aaatgagaaa agcaattaca accagaacac atccaatcac gagtgatccc acggcatcgt
     1981 agattggatt tccggttacg tagctaagcg tgacaaaggt gaaggcaact gcaagaccta
     2041 gaagagcggc ggtgtcttcc ccgagcacga caatgaactc ggcgtttcta gaacctttca
     2101 accacgcaag cagggtcttg gtgcctttga ttttcttaat ctcgcgaatg cagccgtaca
     2161 tagagaagct ttcgagtatt acagaaaaac cgagcacacc tagcgcgatc ggcacgtgag
     2221 cgagaggctc cggtgtggtg agtttgtgga ttccctcacg gatggagaaa atgcccccga
     2281 gtgtaaatag tagaatggcc accaggaagc tccaaaagta cacaacctta ccaaacccaa
     2341 gcggatgatt gcgagttggc ggcatttgcg cgcgatgaag gccaagaaaa agaagcagct
     2401 gattgccgca gtctgcgaag gaatggactg cttctgcaag catactgcct gagccagtca
     2461 ggtatgctgc cacacttttt cccgcagcaa ttccgaagtt agccagaaat gcatatagga
     2521 tcgcgcggaa ggatgattcc tggtgactgc tcatgggttc gttctcctga aatgctgagt
     2581 aattctcgaa tacttgaaaa atattatgaa tctgcgtgtc tcaatcggat gcggtcgggc
     2641 gaaaataaat caacagtaga atcatcgcaa cggaaactgc agcagagcct gaatcgcccg
     2701 ttgaaaaata gacaacaccg ttgaacagtg ctcccgcttc cacaatggcc cattgcacaa
     2761 tcttctgaac aaaattcagg tttccttgcg aatcggaagg ttcacttccc gcattgattt
     2821 cttccttgcc tcccgcaagt aagcgcggga tgaaaaaaga agcggccgcg ctgcccgcgc
     2881 caaagatcca cccgatttgc gcgaggaacg attgtggcgc gttttttggt cctacgaaat
     2941 aggatacgat cagaaaaaaa acaggccccg aacaaatggc cgcatggatg attgagagca
     3001 taggattgcg tttcacgctg tcccattgac ctgattgcac cacggggtca acgccattcc
     3061 tggaaataaa tattgacgct cgcattttgt acacgttccc tgcaatatga acacgccata
     3121 cattctgggc ggcgcccaga cggatttttc aagaaactgg actaaagaag ggaaaacctt
     3181 catgtccatg atgcgcgaag tcgttcaaga cgggctgtct caaattggcc tggattatga
     3241 cgaaatcaca cgtcttaata aagagaacaa aattgctgta ttcgttggaa acttcgatgc
     3301 agaacaatac acccagcagg gccaccttgg cgcattcctg acggaagtgc acccggcctt
     3361 ctacggagtt cccggcgccc gctatgaagc tgcgtgtgct tccggatccg tcgctctcga
     3421 tgcagcatct gccaggattc gcggcggtga ttacgaagta gcggtcgtac ttggaatcga
     3481 aatcatgaag acagtgagct cttcggttgg tggagatttc cttggaactg cagcatacta
     3541 cgaacgcgaa gccaagggag ttcaattccc tttcccaaaa ttgtttggaa aacttgcgga
     3601 tgtgattgtc gagcgcgcca aaggcgttgg cgaaaaacgt atcatggacg ctcttgctga
     3661 aatctcaaga attaactacg cgaatgcgaa actcaatcct cttgctcaaa caagaacatg
     3721 gtttatgagc gaagagcatg cgaaagatcg aggcagtcag tacaacatgg ctgtgggcgg
     3781 aagactctgc attgcagatt gttcccaggt aactgatggc gcggctatca ctgtggtggc
     3841 cagtcctgct tacgctaaac agtttgcgca gaagaaagga attccaatga gcaaactggc
     3901 agtgatcaaa ggctggggcc atcgcgttgc tccggttacg tttgacgcca aggttgctga
     3961 gtctaaagac aacgaataca ttcttccatg gacgcgccag actgtgaagg atgcatacac
     4021 gcgcgcaggg atcgatgtgg atcaaatcga tgtgattgag acgcatgact gtttcacttc
     4081 cagtgagtat gctgcgattt ctgctttcgg aatgaccaag cccggcgaag agtatcaggc
     4141 aatcgaagat ggcaggatcg ctctggacgg gccggccaaa gcgggcaaga agccaatcaa
     4201 tccttccggc ggactgatcg gtgcaggcca ccccgtggga gcatccggtg tgcgtatggc
     4261 tgtggacctt ttccgtcagg tgacagatca ggcaggagac taccaggtgg caggtgcaaa
     4321 gaatggcatc atgctgaaca ttggtggaag tgccacgacc aacatgactt ttgtaatcag
     4381 ccgagccgaa taagcgctct acccgattac actgtgtcag aaaaaaggcc gcgcttgcgg
     4441 ccttttttgt ttcatggggt caaaacatct ggacggtgcg ggctttgaaa caaggatcgt
     4501 tacccgtcat ggcgattcta gatataatca agacccttca atttccactt ccatttgaaa
     4561 ttcatctgcc ggatggcagc catcataaac acggttccgg agaaccagtg ttcgttatgc
     4621 gaatcaagac agatgcagca ctcagcgacg tgatgcgtca gggcagtctt ggtttcggtg
     4681 aagcgtatat gcgtgaggag atcgacatcg aaggagatgt gcaggaagtt tcgcgccttg
     4741 gctacgcgct tatggaaaca gacgcgctca ctccaacttt catggaaaag ctgaagttcc
     4801 ttttcgtgta tttttcccgc agaaacacgc gagagggttc gcgcaaaaat attcaagcac
     4861 actatgatct tggaaacgat ttctattcac tgtggctcga taaagaaatg cagtacacgt
     4921 gcgcatactt ccacgatttc aaagactctc ttgaaaaagc tcaactccaa aagctggaac
     4981 atgtgtgccg aaaggttcgc ctgaaaaaag gggacacagt tgtagaagct ggttgtggat
     5041 ggggtggttt tgcacgttat gcggcaaagc actatggggt taagattcga tcctacaata
     5101 tttcgcaaga acagatcgag tatgctcggg aaatgcagaa gaaattaaaa attcctcaaa
     5161 gccaggtaga gtatgtcctg gacgattatc gcagcattgg atcttcaggt gaaaaatacg
     5221 ataaattcgt atcagtcggg atgctggagc acgttggccg cgaaaactac tacggactct
     5281 atgaaatcat tcaaaaggta gtagagaagc gtggcatggc gatagttcat tccatcggaa
     5341 gaagattccc tcgtaaacca gatccatggc tcgagaaata catctttccc ggatgctaca
     5401 ttccttctct ttccgacatg atcacgccgt ttgaatcgct tcccggtcat gatctgcatg
     5461 tggtggatgt ggagaatctg agatatcatt acgcactcac tctggatcac tggtggatga
     5521 ggcttgagaa gaacgcagag aagattcgca aacaatatgg agaagcattc gtgcgaatgt
     5581 ttcgcctcta tcttcgcgct gcagctgcgg gctttcgcta cgatgaactt acacttttgc
     5641 aagtagttct cagcaatggc agtgacaaca cagcacctct cacaaggaaa catttgtaca
     5701 aatgagcaat caatatgacg tcattgttgt aggcgcgggt ccgggcggat ccaacgcggc
     5761 ccggcatttt gcgaaacagg gttggaaagt tctgatgctg gatcggcaga aatttccgag
     5821 ggtgaagatt tgtgccgggt ggatcactcc gcctgcgttg cgcttgctcg acttgaaacc
     5881 ctccgattat ccgcacacgc tccagcgctt ctcttctggt tctgttgtcc tgggggatcg
     5941 ttactacgaa accgattacg gaacaactgc aagctacggg atcatcagac aagagtttga
     6001 tacatttctg gccagacgtg cgcaagcctc cggggtcgaa tttctggaag aggttcgaat
     6061 caagtcgatc gaacgcgggg atagccccga cgcgctcgtc agggttcgca gcgaagatgg
     6121 aacagagtgg accacaaaat tgatcattgg tgccgggggc acgggatgtc cggtggcgcg
     6181 gcaatgggga gaaaaatcaa aagaagaaat catcgtacag gcaacagaat cagaaaccaa
     6241 gttaggcgcg gatctacttc ggacactcac tccatattat ggaacaacgg aattgttccc
     6301 cgaacctgac ttcttcggat acgcctggtt tgtgaccaaa ggagactggg taaacattgg
     6361 aatcggccgg ttcagccaca gaaccaaaca gctggacaca cacagagacc agttcatgga
     6421 acagctgcgc aaactcggac gtcttaaggg cattgaggat aaaatcgtgc cattcggtcg
     6481 tcacgcatac aagttgtacg atgaagttcc gcgaaaactg ttcggagaca gattcatgct
     6541 gatcggagat gctgccggct ttgcttccaa atgggcgggg gaaggaattc gtccggctat
     6601 tgaaactggc gaatttgcgg cccgagtcgg taacgcggct ctcaaggcac aaaacctgag
     6661 tgccaattcg ctttcacctt acaagcagtt atgtgatcat gcgtatggtc cgcagaaaga
     6721 cacaacgatg gggcgcttga tttccggcct tcccgggagt gttcagaccg gtgtgggaac
     6781 cgtgatttgc aaatccgact atcttagaaa gaagctgatc tttgaagctg cttttggctt
     6841 tgaacctgtg cgcgatcctg tctatcttta gaactggacg cgcactccgg catttgctcc
     6901 gtatgctctc ctggtggaga taccttccgc gaagatcttc gcgggtccga ggttgatgtc
     6961 catactcact tttgcataag gaacgtgttt tggaatcgta cctctgccag aaatattcat
     7021 ggtaaggtaa gatgtaaacg tgttggagat cagattcact cctgcagatg acagcagatt
     7081 gttcagtatg tccgtggacg ggtagaccgg cccaatgcgg gtaagattca tgtccgcata
     7141 accgctgttt gttgtaacgc ccccgccaac agagagactc aaaacataga gcaactgcat
     7201 cccgctcatg atttcaaccg ggtatgtttt gattttcgtc tggatactga gatagttgta
     7261 gccgtcccag aagacctgca cgccgctcaa ggacgcaacg cggtatttct ggtactgctc
     7321 cgcctgaatg ttcagccgag agagatggta gccagtgccc agggaaacgc ccagaaatcg
     7381 aaatagtgga ccaccggcga tgtttgcgcc ttccacgaga tgatagcgaa catcaaaacc
     7441 ccgattgtag aatcccgcga cgaccttcag attatgattg tcccgtgagt aatgctctct
     7501 atattccgct ccggtcgcat atacatcgac gcgagataga gatagcatgg atggagtcga
     7561 accgtttgaa tcatacggat cgtattcatt tccgacgagg tagccaagat tgactcctcc
     7621 gaaaatatgt gggatcgcat acgctccccc ggaaggcacg tcctggaata ctcccactcc
     7681 gggcatggtt agagttttgt tttgaagcgg aacgaatccc gcacttccac tggcgccaac
     7741 agtaaatttt tgtaagttta ccgaacctgt gggggcgctg ctgatgtttg ccagagtgcc
     7801 tgcgcgtgct atatctgaaa aaagctgatt cgtgtactga tactgaaact gaaaaaacag
     7861 gctgttgaga gaatcctgcg aaaacggaat cgcgccgcaa ccagttccct gacagaacgc
     7921 ctgagcgtgc accggactgg caagcgcgat ggaagctgtg acgagaatga ttgccgcgag
     7981 tttgtgtaac atcgtgatcc tctgagcacc accgcgctta tttcaattgt acaatcagat
     8041 caaatggcag ctctttgtct ccatagagag cgaagagcgg aagatcgatc atggattgct
     8101 caccgattgt ttgcagttcg agtttcttct ctactccgtc ctggctaaca gacactgatt
     8161 ttactttgag attttttccc agcacgatct ggtagcgaac agaggagagg aagagtttgc
     8221 cgatttgttt gcccatatcg tctccacctt gagtggctgg ttctgacgtt tcttccgatc
     8281 cttctttggg ggcgtcgggc atctttgttt ctgccggctt ttcttccttt ttgtcttttt
     8341 ttgcaggcgt aaaatggaag atcatctgct tcttcgccgc atcatatctt ggcatgtaag
     8401 gaaattcggc ctcttgaaat ttggagaaat ccagttttag atactgcgga accgcgaagg
     8461 acattcggat gccggagtca taatctgatt gcagcgcttc tactttcaga tcctttgcaa
     8521 tggacccgag cttgcccgga agctcctgtt ttcctttctc cacctcttgc gaaagtccgc
     8581 cgctttcagc cttgtctcct ttctttccca tatcttccag cgctttggag aagcgaaagc
     8641 tccattgaac gttcaaagtt ccattttcct gcagatccag aaaatgcatg atagaaaagc
     8701 agttggttag cgaggcgagg agagtgccca tcgccagtag tttcaagatt ttcaaaattg
     8761 ttctcctcag attctttata ggacgtgcaa aaagacccgt gcgttcgcgc taattcatca
     8821 atttcccagg attcaggttt ccacgcgggt ccagagtttt cttgagagat tgaagcgtcg
     8881 caagagcggc aggcccgact tgcctgcgaa aatggtcgcg atgatcattt ccaattccat
     8941 gatgatggct aatattccca ccgtttgcaa gcatcgcgtc ggacacggca gtcttgagct
     9001 gtgtccattg ctcgactgga tgatcgagat ccatgggaaa catcaccgta aaatagagac
     9061 aaaccccgtc gatgtaagag tgacttgcat ggcaaatccc gatcgcgccg ggaatgctct
     9121 cctggattgc tgcgagcact tcgtcgtgca ctttcatagc gcgcccgtaa ggcaccgccg
     9181 tctccattgt gtccacgcca atcccatact ctagtagata gttgcgcaga aacggcatgt
     9241 tgaagcgtcc tttgagccat ttttttccag ggcttgcgcc ggcgtacatt ccgcggaatc
     9301 gcggagcgag tttctttagt ctggcgattg cttctttgct gtctggacca tccgcgcccg
     9361 cgacgatcag acacttgcgt tccgaaagcc catacgattg aaggacgatg ccctcaattg
     9421 tgtctttggc gaatcgcttg atccgttctc cggcgcctgg attctgaacg tgcgatacga
     9481 ttctgccaaa tgattcgagt tgagatgttt ctccttcatc gctgagacga agcatcgaaa
     9541 aagaaagttc cagctggttt gcttcgcgga tgaactcgag gccggttgcg aaatcgggaa
     9601 agaggaagcc accgtaagtg cgctcagatg gaagctcatg cagttttact gctaccgcag
     9661 tgatgattcc cagagtccct tcgcttccac ataatatttg attcagatcg ggcccggtgg
     9721 aagccgcagg gtaatgcccg gttgtaaaga gtccgcccgg agtgaccatg cgaatggctg
     9781 cgaccatgtt ttcgattttg ccgtacctgc ccgactgctg accggcgctg cgggccgcga
     9841 cccacccacc taacgtcgag tattcgaagg attggggaaa gtgaccaaga gtatagcctt
     9901 gcttggcaag ggcgagttcc aggtcaggtc cgtagattcc tgcctcgaaa gttgcgacag
     9961 aactcacagg gttgagtgcg atgagacgat tcatcctggt catgtccacg cagacgattg
    10021 cctttttgcc accggacttt gcttccactc cacctacaac cgagctacct cctccgaatg
    10081 ggatgatggc ccagtttctc tgcaccgcga gtttgagaag cttctctatt tcaagctccg
    10141 tctctggata tacaactgcg tccacataag ttttcaggag attgccacga aggcgcataa
    10201 cgtcgaaata acttcttccc gctgaatgca gaattcgttc agcgttgtct gtcttgacac
    10261 ggttttttcc aaagattctc tgaagagagg aaagatttgt tcctttgatt tttgattccg
    10321 cagggcgaag atccgtgagg cgaagagaag gagtgatagt ttcctgcatc ttgagcgtat
    10381 ggcgaatgta gttcaaaatc gggccgattc tatccgcgta gaagaagtcg ttgtccagcg
    10441 aaccccacgc attccatcgc aggttttcgc gcctggtgtt gagcgccttt acttcggtgt
    10501 agatcatagc ccgctcctca atggtgcgat tatttgaagc atagcttcgt gaagtaggtg
    10561 atttgttgca agcagggttt tgtccgttag ccgatgttcc tcccccgcaa atgtagtgat
    10621 tctcccaccg gcttcctgta agataacttc tgaagcgcac gtatcccagg ggctcaagtt
    10681 gcattcgtag tgggcgtcaa aacttccttc tgccagccag cataaatcaa tggacgcaga
    10741 ccctgtgcgc ctgattcccc tgcagttctt gagaacagac tcgactccgg cgattaaagc
    10801 ttccaggatt gtagctcgat cgtagggaaa tccagttact acaagagctt gcgatgcggc
    10861 gtttgtttcc gacactcgga tgggattgcc attcttcgtt gcgccgtgtc ccacggcacc
    10921 cttgtacgtt gttcccagag aaggcatatg aacaattccg gccagcggct taccgtcgag
    10981 ggcgatccca acagaaacgc tgtatagagg gagaccgtgt gcgtagttta ctgtgccgtc
    11041 cactggatcg atgatccagg tcaggtcacg accaatgctt ccgcctgttt cctctgccag
    11101 aatggaatct tcgggccagt gttcacgtat gaatgacgtt aggcgatttt cggattccac
    11161 gtctgccacg gtgacgaggt ctttgtcatt tgatttggtt ttgattccca ggtttccgct
    11221 ctggattcga accagggcct ggcttagatc gggcagtacg agcttttcta aacggttgag
    11281 tctttcaatc gcttcgagta cggaactcaa tttgcctcag ggaaacaggc gcagccgccc
    11341 ggctgacagg atctttcggg agccgaactc ttgccgcttg cgcccagaac tgccaggggt
    11401 ttgaatactt gaagagtccg agtggaattg catttttcgc acaccgcaga tttacctgtt
    11461 tctcggtctg cgaggctcat cttgctgtcg aatacctggc cgcaatcctg acactcgaat
    11521 gtgaacattg ccatatgccc agtagaacat tcatttaggc gaggggcaag gaaaaaaccg
    11581 attgcggcag gcgcgaagcc gactgcgatg gatgagttga atattcaaat cattctcatt
    11641 cgacacgggg aaacagaata caatcgagaa ggaagaatcc agggcactct ggattcacct
    11701 ctaacggaaa gaggacgcct cgaatccagc acactgggtg tttctatgcg tgactggttg
    11761 ccacctgttc accaatggct ggtgtcgcct cagggtcggg cccgggaaag ttcgcgtctt
    11821 atccgagatc attttgcaaa tggtgggcag gctgtgcgcg tcttgccggc ggaagaactg
    11881 gaggagtcag cgcgtgagat caactgcggc gattacgaag ggaagatcac ggaaactctc
    11941 gatcagagta tcatccagaa agtaagagag ctcgcggatt ttccttatcc ccagggggaa
    12001 agcctcgtgg atgttatgaa tcgcggcaag ttcgttcttg ctcgagtgat tgaatcgtca
    12061 cagcgcgcgc ataatgcgca tccagatcta aacgaatatc gagtggttct ggtttcgcat
    12121 ggaaatttta ttcgcgcgtt tggagctctc ttgacccggc tggggcctga gtttgcgctt
    12181 cgtgcattcc agagcaacac gggagtgaat cgtctggttt cgcgtgatgg gggtcggggc
    12241 ttcaagattt tgacatggaa cgacaccagt cacatgaatc agcatccaac ggaagccgcg
    12301 aatctgggca ctgcgtaatc agtgttcgaa aatgtcgccg tatcccagtc gatcgagttc
    12361 aatgcgaaca ggaagaaatt caacacagct cttataaaag cccattctgc c
//
LOCUS       DAORFF010000026        12039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2324_length_12039_cov_4.235648, whole genome shotgun sequence.
ACCESSION   DAORFF010000026 DAORFF010000000
VERSION     DAORFF010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12039
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2324_length_12039_cov_4.235648"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PKK76_02070"
     CDS             complement(<1..996)
                     /locus_tag="PKK76_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNL67276.1"
                     /translation="MPGTREEGMAKMERLGCLYLTFLNHPNGLSFSKIRDNLAPAYSG
                     DPESARRKFERDKDELRLLGLDLEHFPDGQALPNGNLARGHIYVPRNEFEKLPDLKMS
                     AEEASALSAVLLYAIENTKDERNAELYRSAATKLLYRDPSLLSRGSSQDFWAPSESFV
                     DHLAPIRSAIRKRLLLEIEYPDKELGSSIRLVEGRGLISHRGRWCLVGFARDANAIRY
                     FYLDRIVSVNVTKTQFRADPRFNIKEHTLHPLTLSIHEAAVIDLLLHEEGKEAMVDFL
                     SGIPDNRVKFGNEKNAARIQLRIKTTNKEALFSFMFRNPGVVVQIGPEDIRKDYLN"
     gene            1084..2073
                     /locus_tag="PKK76_02075"
     CDS             1084..2073
                     /locus_tag="PKK76_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67277.1"
                     /translation="MSVVRGLEFLGRTQLMRYGRTEKTSLQIPVRGESLPAYRYSPAS
                     RLRGTILMVHGMTLRGIDDPRLENLGRSFASMGFEMVAPLFPEVRDLRITAETVSKIE
                     DAVTWLSEDVQGSVALFSASFSGGLSIVAACRAIKNKLSAVCTVGTLGDVDSTLDFLL
                     GQQGIDDYGTLVVLWNFLPVPEDVRTALYIAAADNGLIREPPELGRFVAGMKSTEREL
                     FERLRQDPAFRMERWKEFSSTSQFRELRQNVSPLLHVGKMPFRLVLVHGEADNVIPKE
                     ESEKLDRAFREAGTETQLCVTPLISHGDGSIGLSAAPQAWKLASAMGSFFSAI"
     gene            complement(2299..3741)
                     /locus_tag="PKK76_02080"
     CDS             complement(2299..3741)
                     /locus_tag="PKK76_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydrogenase"
                     /protein_id="HNL67278.1"
                     /translation="METISFKAPEYQSDGSFQEIEYRFEGDEKSGWTIFRNGQKQMEL
                     GEGYTAVKSLYCGVCSTDLARRFLPYPLPQIIGHEVVGIKDNKPVVIEINASPLARHI
                     ETKDPFSLHGLHTHSPERITLGIDRLPGGFAPWILAPVDAIVEVPKQVSALAASLTEP
                     FAASLQGVDATPIKNGDSVAVLGPRRLGMLIIAALAGYRKERKLNFKITALIRHENLR
                     ALCLKMGADEVVDTRNPIPDSTYDVVFDTTGKQEGFAEALRMARRSVSLKSTNGQETL
                     GLKHLTDLVVDEMAILPYNVNQLEYTWPDEGPRSNVNIYVAPGVPETIVSEMKEKDAA
                     TGKKRTYHRVDAKETAVKIRAGMQLEGSIVPRFDLAIASSLAEMDQIIRPLEGIEFSI
                     LRPRGAILFTGSSTSALTDALNQKSMEIRTSRCGDFHRTMKLLSEQPEIAATFEQDMI
                     THKYKLDSIRTAFETAADSNKSVKVIVEAS"
     gene            complement(3743..4024)
                     /locus_tag="PKK76_02085"
     CDS             complement(3743..4024)
                     /locus_tag="PKK76_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67279.1"
                     /translation="MEWEKILRDSVKENAIRELHLRHIPVLKTCENWNDVKEIGLVDH
                     RTKYAHYKGLLVRYGERIYYVPEARMEALAPFRNWTSKVHIKVTDPEKK"
     gene            complement(4043..5539)
                     /gene="gcvPB"
                     /locus_tag="PKK76_02090"
     CDS             complement(4043..5539)
                     /gene="gcvPB"
                     /locus_tag="PKK76_02090"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNL67280.1"
                     /translation="MQNQLSTTRNGQIFEEPLIFERSRPGRIGVSFPADDASLPIEID
                     SSLLRSAEDIHLPEVSEPEVVRHFTRLSTWNYGIDLNLYPLGSCTMKYNPRINEEIAA
                     SAQFADLHPNTPEKYAQAGLRAVYELQEVLKTVTDMPGITLQPAAGAHGELTGLFLVK
                     AYLKKKGDTHKTVVLVPDSAHGTNPASCTLAGFTVRELPSTAEGLLDVEEFKKALTPD
                     VAALMITNPNTLGIFETNIPLVADLLHKNGSLLYMDGANYNAIVGKVSIGKMGVDISH
                     LNLHKTFSTPHGGGGPGAAAVVVSDRVREFLPGPIVEKKESSQGPVYVVTSPSASIGR
                     VKAGPGHFGMIIRALTYILSYGNELHRVAEQAVLNANLVRKSLQEVFEPASGLDTMHE
                     AVFSDAKQRKTGLETIHLAKTLIDYGYHPPTIYFPLSVHGAIMIEPTETESPETIAHF
                     CAVMKDLAARMNGQDESMKSSPTRSFVRRIDEVEAARKPVLNYFTKKA"
     gene            complement(5554..6879)
                     /gene="gcvPA"
                     /locus_tag="PKK76_02095"
     CDS             complement(5554..6879)
                     /gene="gcvPA"
                     /locus_tag="PKK76_02095"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNL67281.1"
                     /translation="MNPHKYLPVSEKEYQEELSSLSLKSADDLFRCIPEALRGNTTTS
                     LRSPMSEMEIREHFRPGVEKIQHTFIGGNGHTHYIPSIIGPLISRGEFLTAYTPYQPE
                     ISQGTLQAMFEYQSMMATLMGVEISNASLYDGSTAMLEACYMAQRITGKSSILFSEQI
                     HPEYIETMRTYSEGGIFQFDFVSADASGRIDVQKLSGKISDNTAALVVQTPNFSGIIE
                     DLTALKKILEAKGILLIAVITEAMSLALVKSPGACGADIVCGEAQSFGVPLSYGGPWL
                     GFLGASPAFMRNMPGRLIGEAKDANGERAFVVTLATREQHIRREKATSNICTNQGLMA
                     LRAAIYLSVMGKHGIRRAAERCARFAAYGRKKASSIAGVQVIFSSSPIFNEFVIETKK
                     DANEVFEHCVEKFGIAPGSVIGKNRLHVSFHEMHSKEIVDRWAEALASA"
     gene            complement(6899..7294)
                     /gene="gcvH"
                     /locus_tag="PKK76_02100"
     CDS             complement(6899..7294)
                     /gene="gcvH"
                     /locus_tag="PKK76_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNL67282.1"
                     /translation="MDSKIPEGYLFTKQHEWAREEGGFLTVGISDYAQNALGDIVYVD
                     LKAPGTKVEAGKTFGTIESVKAAEDLYTPVSGVIDSLNPAVKDKPEAVNKDAYGTWLI
                     KLKDFDKGALSGLMNSADYAKYLASLDGH"
     gene            complement(7324..8424)
                     /gene="gcvT"
                     /locus_tag="PKK76_02105"
     CDS             complement(7324..8424)
                     /gene="gcvT"
                     /locus_tag="PKK76_02105"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNL67283.1"
                     /translation="MNLKKTPLNSIHHSSGGRMVPFAGWEMPVQYSSILQEHQAVREK
                     AGLFDVSHMGEIEVKGTTAEEFLDSVTCNRVQEMQDGQVQYNAVLNEKGGIVDDITIY
                     RVNPESFFVVSNASNYEAVTAHFEKYKIAGTDVQNISNTIHQIAIQGPLAEDILRDFL
                     NLDLSSIGYYRFKDMDSRFGKLRLSRTGYTGEDGFEIYSDVNAGVEIWKGILAQKSSS
                     GLIACGLGARDLLRLEARYPLYGHELNETWTPVESGIGWIVKEKTSVMPGYARVLEQK
                     KSGPANQVVGFKLMEGGVPREGYAVVNESGAELGKVLSGGFSPIVNAGIGTAFLPVGL
                     EAFYIQIRDKKVPAKAEKKAFVQGTAGKNRQK"
     gene            8988..9419
                     /locus_tag="PKK76_02110"
     CDS             8988..9419
                     /locus_tag="PKK76_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67284.1"
                     /translation="MRENPLREVNIALVATMVTLALAVGVDTILPRIGLAKLTAVHYT
                     HSRVFKATPAGGEIVYKGADPARLKEDLEILSRRFERGHFEIITLPGSKLSNEVTAMK
                     ARAASYRYSVSTGAEEATLRVDGPQEPLAGYLKYLQANWNQ"
     gene            complement(9426..10439)
                     /locus_tag="PKK76_02115"
     CDS             complement(9426..10439)
                     /locus_tag="PKK76_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67285.1"
                     /translation="MIAREATARGWLIGFFVLGPATFAIFFLQQMLGFFPAPAHCPHS
                     ITLKVSDAELLADARKISQDCVLIFTSSEGGAVGGGKSISELVHQENTTVVSDTEGYS
                     PFRAIVYLGRMNPAWGRELPYPRMILMINSHYARDHEYGSDLFMQYFPEKESANFYKG
                     QAPFPLSQSVGENRSFFTVLAIYRGWMRRRIGLPLERILAGEKTFAKEADLTTDPKWN
                     VSTSISQKIEPESLRKERTIPNAHGLFESALSHLPRERVKLIVMPLHTVVYGNLGLSP
                     MENERNMTSAFTRSGLPYRIVRELDQPGYWFDTIHFNERGRKALAQILAEESTQMMRT
                     KPR"
     gene            complement(10439..10561)
                     /locus_tag="PKK76_02120"
     CDS             complement(10439..10561)
                     /locus_tag="PKK76_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67286.1"
                     /translation="MIPKLRLTLSFAVGLLFGLLLYFLLFQSTSEIHHFLYEAF"
     gene            complement(10566..11273)
                     /locus_tag="PKK76_02125"
     CDS             complement(10566..11273)
                     /locus_tag="PKK76_02125"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNL67287.1"
                     /translation="MHDAATAKTSVSEFIRRSFLFPSFLCGPIVLSGQTWIREKFLRP
                     QDVWLICLGLIKVLILLPFWNAHFETGLLLPFQPDVSGFMNLLKTGIYRYAQLYLDFS
                     GIIDIAAGVSGLLGMRIRHNFNRPWLASSVGDFWRRWHITLGAWMRRHVYIPLGKTRF
                     ALFLVFLLVGAWHGLKLHFILWGALHGLAIITERFASSHWSLPEFLARHTIAIRGLRI
                     VLTQLFVTLSWVLFFWA"
     gene            complement(11578..>12039)
                     /locus_tag="PKK76_02130"
     CDS             complement(11578..>12039)
                     /locus_tag="PKK76_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67288.1"
                     /translation="GKPSIAGDLIFASSRFIFAGSDDSTSATPPSRSSFTTDLGATTW
                     TADSTNKIFAGNAAGEYARALAYNGTRLEAVGDNCRVDFTTNLTSLSWSSTALTMSGC
                     SAINWKGMIWDGSKFVAVGSSGKFAASATGASTDWTYTTLGSSNVNAIAVR"
BASE COUNT         2836 a         2881 c         3085 g         3237 t
ORIGIN      
        1 attcaagtaa tcctttctga tatcttcagg accaatctga acgaccacac ctggattgcg
       61 aaacatgaaa ctgaagagcg cctctttgtt tgttgttttg attctgagtt gaatgcgtgc
      121 tgcattcttt tcgtttccaa acttgaccct gttgtccgga atcccggaca gaaagtcgac
      181 catggcttcc ttcccttcct cgtgaagcag tagatcaatg actgcggctt cgtgaatgga
      241 cagagtgagc ggatgaaggg tgtgctcttt gatattgaat cttggatctg cgcgaaactg
      301 ggttttagtc acgttcacgg aaacaattct atccagataa aagtagcgaa ttgcatttgc
      361 gtcgcgagca aaaccaacca ggcaccagcg tcctctgtgt gaaatgagtc cgcgcccttc
      421 taccaggcga atgctcgatc ccagttcttt gtctggatat tcaatctcaa gaagcaatcg
      481 tttgcggatg gcggaccgaa tgggcgccag atggtccacg aaggactcgg aaggggccca
      541 gaaatcctga gaacttccac ggctcaggag agatggatcg cggtagagca gcttggtggc
      601 agcggatctg taaagttctg catttctttc atcttttgta ttttcaatcg catacagtag
      661 aaccgcagac agcgcgctcg cttcctctgc agacattttg agatccggca atttttcaaa
      721 ttcgtttcgc ggaacgtaga tatgtccgcg cgcaaggttt ccgttcggaa gagcctggcc
      781 atccggaaaa tgttctagat ccagacccag caatcgcagt tcatctttgt ctctttcaaa
      841 ctttcttcta gccgattcag gatctccgga gtatgcgggg gccagattgt cgcggatttt
      901 agaaaaactc agtccatttg gatgattcaa gaatgtcagg tagagacatc cgagtctctc
      961 catctttgcc attccttctt cgcgagtgcc cggcatgcct gggtaaggca catcgccagt
     1021 gttgtcggtc aaggaaaaag aacaaaatga ttgcgagcgc gctatgctct ctagactttc
     1081 ggcatgtctg ttgttagagg gcttgagttt ctgggcagaa cgcagttgat gcgctacggt
     1141 agaacggaaa aaacctctct gcagattcca gttcggggag agtctctgcc cgcgtatcgt
     1201 tattctccag cttctcgctt gcgcggtacc atcctcatgg tccatggcat gactcttcgc
     1261 ggaattgatg atccgcgtct ggagaacctg ggtcgatctt ttgcgagcat ggggtttgag
     1321 atggtggctc ctctttttcc cgaggtgcgc gaccttcgca tcacagcaga aactgtttct
     1381 aagatagaag acgcagtgac ctggctttca gaagacgtgc aaggaagtgt ggctcttttc
     1441 tctgcttctt tttctggcgg gctttccatt gtggctgctt gcagagcgat taagaataag
     1501 ctgagtgctg tttgtactgt gggcactctg ggtgatgtgg attctactct ggactttctt
     1561 ctggggcagc aagggatcga tgattatggc acactggttg tgctgtggaa ttttctgccg
     1621 gtgccagaag atgttcgcac tgctctctat atcgcggctg cagacaatgg actcatacgc
     1681 gagccgcctg aactcggaag atttgtggca ggcatgaaga gcacggaacg agaactgttt
     1741 gaaagactca gacaggaccc cgcgtttcga atggaaaggt ggaaggagtt ttcaagcacc
     1801 tctcagttca gagagctcag gcagaacgtt tctcctcttt tgcacgtggg taagatgcct
     1861 tttcgcctcg tgctcgttca cggagaagcg gacaacgtaa ttccaaaaga agaatcagag
     1921 aaattggacc gggcttttag agaagcaggt acggaaacgc aactgtgcgt cactccgctt
     1981 atttcgcacg gcgacggttc gatcgggctt tctgccgcgc cgcaggcctg gaagctcgct
     2041 tctgcgatgg gatctttttt ttctgcgatt taggttttgc agcgggcttc gtggttttct
     2101 ttcgctgagc cgattttgtt ttctttctgg tgggctttgg atctggtttt gcggatttgt
     2161 gaattgtgtc tgatagactg cggtcttcgc tgaggatggc agtgcgaaga gattctgcca
     2221 gttgttcttt ttcctccggc aatgcagcaa gcacgctgtc tttctgagaa atttcgccag
     2281 atggtttgcg cgaagccatc agcttgcctc tactatgact ttgacgcttt tgttcgagtc
     2341 agctgcagtc tcgaatgcag tgcgaatgct atccagtttg tatttgtgcg tgatcatatc
     2401 ctgttcgaag gtcgcggcga tctctggttg ttcggacaag agtttcatgg tgcgatggaa
     2461 atcgccacag cgagacgtgc gaatttccat ggatttctgg ttgagtgcgt ctgtcagtgc
     2521 ggacgtagac gaaccggtaa agaggatggc gcctcgcggg cgcaaaatag aaaactcgat
     2581 gccttcgaga ggacgaatga tctggtccat ctctgcgaga ctggaggcga tcgcaagatc
     2641 gaacctggga acaatcgatc cttcgagttg catgccagcg cggatcttaa ctgcggtttc
     2701 ttttgcgtct actctatggt aggtacgctt tttacctgtg gccgcatctt tctctttcat
     2761 ctcagaaaca atcgtttccg gaactcctgg agccacgtag atgtttacgt tagagcgagg
     2821 tccttcgtcc ggccatgtat attccagctg atttacattg taaggaagaa ttgccatttc
     2881 atctacgacc agatctgtca gatgtttgag gcccagagtc tcctgtccgt ttgtggattt
     2941 cagagataca gatcttcgcg ccattctcag cgcttctgcg aatccttcct gctttcctgt
     3001 agtgtcgaac accacgtcat atgtggaatc agggatagga ttgcgagtgt ccacaacctc
     3061 gtctgcgccc atcttgagac acaatgcgcg taagttttca tgacgaatga gagctgtgat
     3121 tttgaaattc agtttgcgct cttttctgta cccggcgagt gccgcgatga tgagcattcc
     3181 caatcttcgc ggtccaagta ccgcgacact gtctccattc ttgatgggtg ttgcgtccac
     3241 tccctggaga gaagcagcaa atggttctgt aagagaggca gcaagtgcgg agacctgttt
     3301 gggtacttct acaattgcat ccaccggtgc aagaatccac ggagcaaatc ctcctggaag
     3361 tctgtcaatt cccagagtga tcctttcagg cgaatgagtg tgaagaccgt ggagagagaa
     3421 tggatctttt gtttcaatgt gacgcgcgag tggagatgca ttgatttcaa tgacgactgg
     3481 tttgttgtct ttgatgccaa ctacttcatg tccgatgatc tgaggcagag gatacggcag
     3541 aaatcttcgc gcaagatccg tagagcacac gccgcagtag agagatttta cagcagtgta
     3601 gccctcaccg agttccatct gcttctggcc gtttctgaaa atggtccagc ctgacttttc
     3661 gtctccctcg aagcggtatt caatctcttg aaagctgccg tcgctttgat actcgggagc
     3721 tttgaaagag attgtttcca agttatttct tctctgggtc ggttactttg atatgaacct
     3781 tggatgtcca gttgcgaaag ggtgctaaag cttccatacg agcttccggg acgtagtaga
     3841 tgcgttcgcc gtagcgaacg agaaggccct tgtagtgagc atatttggtt ctgtgatcga
     3901 ccaggccgat ctcttttacg tcgttccagt tctcacaggt cttgagaact ggaatatgcc
     3961 ggaggtggag ctcccggatt gcgttttcct ttacggagtc cctgaggatc ttctcccatt
     4021 ccatccatta gtttccaaca gatcaggctt tttttgtaaa gtaatttagt accggctttc
     4081 gagcggcttc cacttcgtct atgcgacgaa caaagctccg cgtcgggctg gacttcatac
     4141 tttcgtcctg gccattcatt ctggccgcca ggtccttcat cacagcgcag aaatgcgcga
     4201 ttgtttccgg agactctgtc tcagttggct cgatcatgat cgccccatgg acgctgagcg
     4261 ggaaatagat ggtaggtgga tgatatccgt agtctatgag agtttttgca agatgaatgg
     4321 tttcgaggcc tgttttacgc tgttttgcgt cgctgaagac cgcttcatgc atcgtgtcca
     4381 ggcctgaggc tggctcgaag acttcttgca gagactttcg cacaagattc gcgttgagca
     4441 ctgcctgctc ggcgacgcgg tgcagttcat tgccataaga cagaatataa gtcagagcac
     4501 gaatgatcat tccaaagtga ccggggccag ctttgactct tccaatggac gcggaaggcg
     4561 atgtgacaac atagaccggg ccctgtgagc tttctttttt ttccacaatg ggtccaggta
     4621 gaaattcgcg caccctgtca gatacaacga ctgccgccgc acctggtccg ccacctccat
     4681 gaggagtgct gaatgttttg tggagattca gatggctgat gtccacgccc attttgccaa
     4741 tagacacctt gcctacgatg gcgttgtagt tggctccgtc catatagaga agagaaccat
     4801 tcttgtgtaa aagatctgcc accagaggaa tgtttgtttc gaagatgccc agagtgttcg
     4861 ggttcgtgat catgagagca gccacatctg gagtgagcgc tttcttgaat tcctctacat
     4921 ccagaaggcc ttccgcggtg gagggcagtt cacggacagt gaagcctgcc agagtgcagg
     4981 aagcaggatt ggttccatgt gcggaatccg ggacaagcac cactgttttg tgcgtatcac
     5041 ccttcttttt taagtatgct tttacaagga acagcccggt cagctcaccg tgggcacctg
     5101 ccgcaggctg cagcgtgatc ccgggcatgt ctgtgactgt cttgagcacc tcttgcagtt
     5161 cgtagactgc gcgaaggccc gcctgtgcgt atttttcagg agtgtttgga tgaaggtccg
     5221 cgaactgagc gctcgctgca atttcctcgt tgatgcgagg attgtatttc atcgtgcagc
     5281 ttcccagagg atagaggttc aaatcgattc cgtagttcca ggtggagagt cttgtgaagt
     5341 gtcgcaccac ttccggttca gaaacttccg gaaggtggat gtcctctgcg ctgcgaagaa
     5401 gagatgaatc gatttcaatt ggaagagatg cgtcatccgc agggaagctc acgccgattc
     5461 ttccagggcg agatctttca aaaataaggg gttcttcgaa gatctgcccg ttacgagtgg
     5521 tgctcagttg attctgcatt gcttttctcc ggcttacgcc gatgccaggg cctctgccca
     5581 gcgatctacg atttctttcg aatgcatttc gtggaacgaa acgtggagtc tgttcttgcc
     5641 aatgacgctt ccaggtgcga tgccgaattt ttccacgcaa tgctcgaaga cttcgtttgc
     5701 gtctttcttt gtctcgatta caaattcatt gaaaatggga gaggaactga agattacctg
     5761 cacacctgca atcgaggacg ctttctttct gccgtaggct gcgaatctgg cgcatctttc
     5821 tgcggcgcga cgaattccgt gtttgcccat cacagataga tagatggccg cgcgaagggc
     5881 catgagaccc tggtttgtac agatattgga agttgctttc tcgcggcgaa tgtgttgttc
     5941 tcgcgtggcg agagtgacta cgaatgcgcg ttctccgttt gcatcttttg cttctccaat
     6001 caatcttcca ggcatatttc gcataaaagc gggagatgct ccgagaaatc cgagccatgg
     6061 tccgccgtag gagagtggaa ctccaaagct ctgtgcttct ccacatacaa tgtctgcgcc
     6121 gcatgcgcct ggagatttca ccagtgcaag agacattgct tccgtgatca ctgcgatcaa
     6181 cagtatacct tttgcttcta gaattttctt cagtgcagtg aggtcttcta tgatgccgga
     6241 aaagttgggc gtttgcacaa cgagtgctgc tgtgttgtca gaaattttcc ctgaaagttt
     6301 ttgaacgtcg attctgccgg atgcatctgc agaaacaaaa tcgaactgaa aaattccgcc
     6361 ctctgaatac gtgcgcatgg tttcgatgta ttctggatga atttgttctg agaagaggat
     6421 ggatgatttc cccgtgattc tctgtgccat gtagcatgct tcaagcatcg cagtggagcc
     6481 atcgtagaga gacgcattcg aaatttccac acccatgagt gtggccatca tgctctggta
     6541 ttcgaacatg gcctgcagtg tgccctggct gatttctggc tggtagggtg tgtatgctgt
     6601 gaggaattct ccgcgcgaga tgagcggacc aatgatggag ggaatgtagt gagtgtgtcc
     6661 gtttcctcca atgaatgtgt gttggatttt ttctacgcct ggacgaaagt gctcgcggat
     6721 ctccatctct gacatgggcg aacgcaacga agtggttgta tttccgcgaa gagcttccgg
     6781 aatgcagcga aacaaatcgt ctgcgctctt caaagagaga gacgagagct cttcctggta
     6841 ttccttttca gatactggga gatacttgtg tgggttcatt tgctactgcc tttagaaatc
     6901 aatgcccgtc gaggctggcc agatacttcg catagtctgc cgaattcatc aggccggaaa
     6961 gcgcgccttt gtcaaagtcc ttgagtttga tcagccaggt tccgtatgcg tctttgttga
     7021 ccgcctccgg tttgtctttc acggcggggt tcagggaatc tatgacgcca gagacaggag
     7081 tatagagatc ttctgctgct tttacggatt cgatggtgcc gaaagttttt cctgcttcta
     7141 ccttcgtgcc gggagccttc aaatctacgt atacaatgtc tcccagggcg ttctgggcgt
     7201 agtcagagat cccaacggtc aaaaagccgc cttcttctct ggcccattcg tgttgttttg
     7261 taaaaagata gccttctggt atttttgagt ccatctagat tctcctgatt gcccggctga
     7321 gggtcacttc tgccgatttt tcccggccgt cccctgaaca aacgcttttt tctctgcttt
     7381 cgcaggaact tttttgtcgc gaatctgtat atagaaagct tcgagcccta caggcagaaa
     7441 ggcagttcca atccccgcgt ttacaatagg agaaaagcca ccagaaagaa ctttgcccag
     7501 ttccgcgccc gactcattca caactgcata tccttcgcgc ggaacgcctc cttccatgag
     7561 tttgaagccc actacttgat tggccgggcc actcttcttt tgctccagca cgcgtgcata
     7621 accaggcatt acggaggttt tctcttttac aatccaaccg attccacttt ctacaggtgt
     7681 ccatgtttca ttgagttcgt ggccgtagag aggatagcgc gcttcgagtc gaagcaaatc
     7741 gcgtgcaccg agtccacatg caatcaaccc gctgcttgat ttttgtgcaa gaatgccttt
     7801 ccagatttcc actcctgcgt tcacatcgga atagatttca aatccgtctt ctccagtgta
     7861 accggtgcga ctcagtctga gttttccaaa tcttgagtcc atatctttga agcgatagta
     7921 accaatggaa gaaagatcta gattcaagaa atcgcgaaga atatcttctg ccagtggtcc
     7981 ctggattgca atttgatgaa tggtgttgct tatgttctga acatctgtgc cggcgatttt
     8041 atatttctca aaatgtgctg taactgcttc atagttagaa gcatttgaga caacgaagaa
     8101 actttctgga tttacgcgat aaatcgtgat atcatccacg attccgccct tctcattcaa
     8161 tactgcattg tattgaacct gtccatcttg catttcctgt acccgattgc aggtgacaga
     8221 atccaggaat tcttcagcag ttgtgccctt tacttcgatc tctcccatgt gtgaaacatc
     8281 aaatagtcct gctttttcgc gtacagcctg gtgttcttgc aggatagaag agtactgaac
     8341 aggcatttcc catccagcaa acggaaccat tctgcctcca gatgaatgat gaattgaatt
     8401 cagcggtgtt ttttttaggt tcatttggaa aattccgtgg attggatttt ccagaagtaa
     8461 atttgcacgc tgcttctgct ataaattcgc gcacgattta gcgaaaattt ttttttggtg
     8521 caagttcttc ataattgttt tgaccgtcgg agcccgtttg atttcatcgc atcttcttga
     8581 agcggattgt ttatctcctc agagagggtg tgaattgtcc gtgtataatt ttctgtcaca
     8641 ttagttttgc gaaattcgcg gagagaatgt gagaacgcaa tactacgaag aacgattgtt
     8701 gaatacatga atgagatgaa ggaaaagact gagcaatcaa tgcaaaagaa gcagtcctgt
     8761 gcacaatgcg gggaaacatc tgataaattg tatcagcaca atctctgcaa agaatgcttg
     8821 aagcagagtt ttcagcgtct gattccaatg atcaacgaat tccgcatccg caattgagtt
     8881 ctgaacatcc attgaagcgt cagattagct ctgcccccac caatctctgc atacgtttcc
     8941 tctgaaaatt tactgacagc acgcgcgcct ggccgagttt tgaatccatg cgtgagaatc
     9001 ctctaaggga agtgaatatt gctctggttg ccacaatggt gactctggca cttgcagtgg
     9061 gtgtagatac gattttgccc agaattggtc tggcaaagct cacggcggtc cactatacgc
     9121 attcgcgcgt cttcaaagcc actccagctg gcggagaaat cgtttataaa ggcgcggatc
     9181 ctgcgcgact gaaagaagac cttgaaattc tttccagaag gtttgagcgc gggcattttg
     9241 aaatcattac tttgcccgga agcaagctct caaacgaagt cactgctatg aaggcgcgtg
     9301 ccgcttctta tcgctattct gtgtccacgg gcgcagagga ggctacgctg cgcgtagacg
     9361 gcccgcagga gcctctggca gggtatctga agtatctcca ggcaaactgg aatcaataaa
     9421 acgcctcatc tgggctttgt tcgcatcatt tgagtagatt cttccgcaag aatctgcgcc
     9481 agagcctttc tgccgcgttc gttgaagtga atcgtatcaa accagtatcc cggttgatcc
     9541 agttctctta cgattctgta gggaaggccg cttcgagtga aagcagatgt catgtttcgt
     9601 tcgttttcca ttgggcttag ccccagattt ccataaacca cggtgtggag aggcatgacg
     9661 atgagtttca cacgctcgcg cggaagatgg gataaagcag attcaaatag tccgtgagcg
     9721 ttgggaatgg ttctttcttt cctcaggctc tcaggttcta tcttttgtga gatagaagtg
     9781 gatacattcc attttggatc cgtggtcaaa tccgcttctt tcgcaaatgt cttctcacct
     9841 gcgagaattc gttccagggg aagaccaatt ctccggcgca tccagcctct gtagatcgca
     9901 aggaccgtga aaaaactgcg attctctccc accgactgcg aaagtggaaa gggggcctgt
     9961 cctttgtaga aattcgcgga ttctttttcc ggaaaatact gcataaacaa atcggatcca
    10021 tattcgtgat cccgagcgta atgtgaattg atcatcaaga tcatcctggg atacggcagt
    10081 tcacgccccc aggcagggtt cattctgccc agataaacga tggcgcgaaa cggagaatat
    10141 ccttccgtgt ctgatacgac tgtggtgttt tcttgatgaa ccaattcaga aatggatttc
    10201 cctcctccaa cggctcctcc ttccgagctt gtgaagatga gaacgcaatc ctgcgagatc
    10261 ttgcgcgcat ctgccagaag ttcagcgtct gaaaccttga gcgtaataga atgcgggcag
    10321 tgtgcgggag ccggaaaaaa accaagcatt tgctggagaa aaaagattgc gaacgtggcg
    10381 ggcccgagaa caaaaaaacc aatgagccat cctctggctg tcgcctcgcg cgcgatcatc
    10441 aaaacgcctc gtatagaaaa tgatgaattt cagaagtaga ctgaaagaga aggaagtaga
    10501 gtagaagccc aaaaagcagg ccgactgcga acgaaagagt gagtcttagt tttggaatca
    10561 tattgctacg cccaaaaaaa caaaacccag ctcaatgtga cgaatagctg agtcaaaacg
    10621 attctcaatc ctcgtattgc gatggtatgg cgagccagga attcaggaag gctccagtga
    10681 gaggatgcga aacgctccgt gatgatggcc agcccatgta gagctcccca caaaataaaa
    10741 tgcaacttca aaccatgcca tgctcccacg agcaagaata caagaaagag tgcgaatctg
    10801 gttttgccca gaggaatgta gacgtgtcgt cgcatccatg cccccagagt gatgtgccat
    10861 cttctccaga aatcgcccac agaggaggca agccatggtc tattgaaatt gtgtcggatg
    10921 cgcattccaa gtaggccact cacacctgct gcgatgtcta tgatgccaga aaagtccaga
    10981 tagagctgag cgtatctgta gattccagtc ttgagcaagt tcatgaatcc agagacgtct
    11041 ggttggaaag gcaggagaag tcctgtttca aaatgagcat tccagaaagg cagtagaatc
    11101 aaaaccttga tcagtccaag gcagatcagc cagacatctt gcggcctcag gaatttctcg
    11161 cgaatccacg tttgtcctga aagtacgatc ggtccgcata gaaaagacgg gaaaagaaag
    11221 gagcggcgaa tgaattctga aacgctggtt ttcgcagtcg ccgcatcatg cattctcaaa
    11281 ataatgtaag agtatccagc ggtcgccagt ggctcccctc taagaaaagc agggaaaaag
    11341 aggaatccaa acgcagcgat ggtgcgagcg gtccgccagg gaatgacata aaacgcgagc
    11401 gcggcgagcg gaattacaag tgcgactgtt tcgtttaaac taaaaaaaaa accgcagtcg
    11461 cagaaagcgt gatcaaagga aaagcgcgcg aggacgcggc gctcttaccc gacagaacgc
    11521 tgatcagaac aagaacgccc gtgaaaagga acgtgagctg gagtacgctg aaatccatca
    11581 gcgcactgcg attgcattta cgttcgaaga accaagggtc gtatacgtcc agtccgtgct
    11641 agcacccgtc gcagatgccg caaatttacc gctgcttccc actgccacaa acttgcttcc
    11701 gtcccagatc attcctttcc agttgatcgc gctgcatccg gacatggtga gtgcagtgga
    11761 actccaggag agagaggtca ggtttgttgt gaaatccact ctgcaattgt ccccgactgc
    11821 ttccaggcga gtcccgttgt aggcaagtgc tcttgcatat tcgcccgccg catttccggc
    11881 gaaaatcttg ttcgtgctat ccgcagtcca cgtcgtagcg cccaggtccg ttgtgaagga
    11941 agatctagaa ggcggagtgg cacttgtgga gtcgtccgag cctgcgaaaa taaaccggct
    12001 gcttgcgaag atcaaatctc ccgcaatcga aggttttcc
//
LOCUS       DAORFF010000027        11808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2394_length_11808_cov_3.986472, whole genome shotgun sequence.
ACCESSION   DAORFF010000027 DAORFF010000000
VERSION     DAORFF010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2394_length_11808_cov_3.986472"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            307..525
                     /locus_tag="PKK76_02135"
     CDS             307..525
                     /locus_tag="PKK76_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67289.1"
                     /translation="MAQALKLRVQCHACGYMIEGTAKYGEGHYVGEGVPFDFVAIGKV
                     INSKGKRVVKGEVTCICPKCTVRNKYNV"
     gene            651..2480
                     /gene="thrS"
                     /locus_tag="PKK76_02140"
     CDS             651..2480
                     /gene="thrS"
                     /locus_tag="PKK76_02140"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNL67290.1"
                     /translation="MAKAKQPAEIPVSTMRHSAAHVLAQALREKFPKVDLAIGPDTDH
                     GFFYDVMVDPPLRSEDLPDLEKRMKRIVKANQKFERFYLPIDEAIERLEKEGQGYKVE
                     MARDLKAQGETELSFYENTVKQGGAEKSAVFVDMCNGPHVEHTGQLGAFKLVSVSGAY
                     WKGRTDQPQLQRIAGYLFATEEELRTHERNVEEAKKRDHRRLGVDLELFTTSEKIGPG
                     LILWLPRGNVIKEELEGWAKDTEEKHGYQRVTTPVITKEDLFYTSEHLPHYKESMFPP
                     MRLDEENYYIKPMNCPFHHTIFSMRPRSYRELPLRIAEYGLCHRYEDSGSLMGLMRVR
                     GMSMNDAHIYCSEDDAVEEFLEVLKLHEYYYRTLGIEKYHMVLALRNPKNDKYHGDSE
                     MWARAEALTRKAMETSGIAYEVENDGAAFYGPKVDFQIVSSIGREFTASTCQLDLFMP
                     EKFDLKFVDKDGSMKRPACLHRSPLGTHERFVGFLIEHFGGAFPLWLAPEQVRILPVS
                     ENVHAYCQELASACKKAGLRVTLDDSGETLGKRIRNAEKMKIPRMVVIGDVEKQKNVL
                     NSRDYFSGEKTEISLDAFIAEALDEIRTKKLRQIAKPLLVDAG"
     gene            2559..2978
                     /locus_tag="PKK76_02145"
     CDS             2559..2978
                     /locus_tag="PKK76_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNL67291.1"
                     /translation="MSNLKGSKTHENLKAAFAGESQANRRYLYFAKQADVEGYPDLAG
                     LFRDTAEGETGHAHGHLEYMRKVGDPATGMPIGDTALNLKSAIAGETHEYTDMYPGMA
                     KTAREEGFTEIADWFETLAKAEKSHAGRFEQGLKTLV"
     gene            3038..4405
                     /locus_tag="PKK76_02150"
     CDS             3038..4405
                     /locus_tag="PKK76_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNL67292.1"
                     /translation="MNARINYENTSDTVYDPGNSLYWDVDSTDRELARAFDLCNGCRM
                     CFKYCPSFPTLFQLMDEKQDVFALSAEDKDKIVGECYQCKICYVKCPYTDRDHHKFNL
                     DYPALLQRAVHVKARKEGVPFRDKFLANSDFAGKTAGLLSGLANKAMKSNFHRRIMEA
                     ILGIHRNKRMPEFHSTSFMRWFKKKPAVPVQAGQEKVVLFSTCFVNYNNPQLGRDTVE
                     VLEKNQVHVDCPEQNCCGMPGLNSGDLDWAISKMKKNVESLYPYAKEGYRILAINPTC
                     SLTLKKEYVSFLPPELKEKAQAVASATMDVHEYLFELKKQEKLNRDFNSSPGKVGYHV
                     PCHLRAQNIGYRSRDILRLIPGAEIAVVDECCGHNGTWAMKEEYFEMSLEAGKRAFDG
                     LKEKEASHIATDCPLAAIQIEQGMGLGERPDHPVQILARAYRKPEDGGFPDAISSEEN
                     GQTGS"
     gene            4420..4998
                     /locus_tag="PKK76_02155"
     CDS             4420..4998
                     /locus_tag="PKK76_02155"
                     /inference="COORDINATES: protein motif:HMM:NF023432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HNL67293.1"
                     /translation="MKKLEMADVIPNTEYEKIRDSFRSKILRIKEPRRIAVGPYLMFL
                     FENRETMLYQVQEMMRAEGIRAEEAIQHEINTYNELVPGPCELRATLMIEFADEATRR
                     VKLKELVGLEKHVSLVIDRDYVISAVFDEKQMDPDKLSSVQYIHFPLGEKAAEAFLST
                     NDVELVTTHPACSYRQSISQEQLSSLKEDLYT"
     gene            4913..6490
                     /locus_tag="PKK76_02160"
     CDS             4913..6490
                     /locus_tag="PKK76_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNL67294.1"
                     /translation="MLQLIRPAVIGRAFRRNSSLLSKKICIPEARSFASVVASILILF
                     LSGSIQAEDVAVARRAVVRIDISFFEYSYDTPYRAPAVRRASGTGFIIAGKRILTNAH
                     VVSQSNTIRLHRPDQRQDYPAHIVFIAHDCDLALLEADDDAFYKDTQPLEIGVSPELN
                     SPVVVVGFPIGGDRVSITRGVVSRKDMDTYAHSTVDSHATIQVDAAINPGNSGGPALQ
                     NDKVIGVAFQSLTRGENLGYLIPPEVIRKFLTDVSDGRYDGYIDFGTMDMTTKNPILR
                     SALGLDADSVDTGVLVYDVLQGSSADGFIERGDVLLQVNGKKLSFEGEVDWNGGLHPY
                     IEMLDNLASGTIIEVELLRKGKKVTVKFPARKSKVVDALRINYDTAPPYWIEAGHVFQ
                     PLDANLMQQNSRNWAREGRADLTYYYNNFLSAGIYRSVDGFVMLTQRLADPSNIYNDR
                     FVSQVVESVNGEKVKSFTHFSSLMAAAHKEPKITIRFRDSAVPLVLETASLSQTNERI
                     RKAYGLTSLNFVPGEKK"
     gene            6487..7938
                     /locus_tag="PKK76_02165"
     CDS             6487..7938
                     /locus_tag="PKK76_02165"
                     /inference="COORDINATES: protein motif:HMM:NF036494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67295.1"
                     /translation="MIRSLFSLFLLIPGVSLPDADVRRGIVLLTITPVVEDATRPWIK
                     DQGTPFQRLGLVVGKGKILALESEVRGAAIVEVRKHSSTEAVTATIERSDRETDLAIL
                     SVPADFAQDLNPIPAGPDPVVGETLESIRVDGGLFRLQRNEVQFSEIEVTTATGFTAF
                     PVGNVASLEPFPEGGILISKGKLAGFVHDRSNGRRIDFLLPGYIHQFLAGGAPGSAGF
                     DYESLVDPVLRRNAGVEGRSGPMVTRIYPGTSAYGVLQKGDIILAVDGVVLDERGFFE
                     DGKNGRQRIELLLYAKGGVTRKIGDTVPIRIIRAARRMDVSFKLIRNEGNAVRVPIRE
                     REQGFLVEGGVVFLNLSLFFLQSQFGATYATSAPELAYLYQSKRFRDQPGDDRIVIIA
                     RVLPDDMTLGYESLGGAQVLRAGNETVKSVNHLHDIMEAAAKKDATLTLELSNGRMMV
                     LDLKNRNAINSRIRKRYGVPVDFRRASNSGPGG"
     gene            complement(7944..8714)
                     /locus_tag="PKK76_02170"
     CDS             complement(7944..8714)
                     /locus_tag="PKK76_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67296.1"
                     /translation="MLASSPALAQPAPLTGMVGAGMGYYERRTSDQSIRAYTVPVFLP
                     LEATLGRFYLTGSLEYARREMEGATEASSEGLNFVNMEGRFRAIDSRNLRLTFMEIAG
                     VSTARGSSSLPPESRLADGYRFDSAMSLQYRIERLSFELKGGQIANRTRGDFRPGDIR
                     SGGLSMGFGLGAYNNEAWPVNLVLGVTSQFYGADRFAGEDLPGTEYGTVFFSPGLMLS
                     GKSLALRAGLDFPIRHLGAEDAYRDKVRANIGLRYFLQ"
     gene            8887..9801
                     /locus_tag="PKK76_02175"
     CDS             8887..9801
                     /locus_tag="PKK76_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67297.1"
                     /translation="MSEFVGKLWANPALAGASVVRRENQILSFIRENQTQLQQAFAKP
                     GYFPDLGWDGSIRLLFSVLTDLIVDSVEPRLDKILGALLNPEVLPQLEIRPQEVDRER
                     IKDFILKGLRNKATRDPYIAVLETISYGLYDRYVPKALARHKVIHNEFYRKDRLSLPA
                     PIMIAYLQLCSLFRPLYWQKVATASNNQLNLAAVAKDPRMFDSTYKFLNAFLVQEMGG
                     VPDRILRVGLESCMNAEEHPDTGGSSRLIGILVARAAEFDPTQKQDRGAESPDKSWFH
                     IHKKTAKYYGYDARYLDELYQIASEEAW"
     gene            9804..10970
                     /locus_tag="PKK76_02180"
     CDS             9804..10970
                     /locus_tag="PKK76_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNL67298.1"
                     /translation="MSSWKKAYNPDHLPGFIETGIILLIILVIGHTITEDIAVIYHWD
                     HRWMFYLTVAGFVFDLIFTAEFAARSIITLKHGHFKHYVTQERGWIDALTSFPLLLLV
                     SGPALVVYLTDAHAEGAFFSFLIILKTAKAIRVTRVLRLIRVIKIFGKIQNTESQMTN
                     RHVATISTIAVVSLVSVLVIAQFVPFLHIGDHEAYRQRRVSELSSLFQAASAFPEGVD
                     RSSGIQKMIRAEGKGDVIKIKRANGEVVFEADPGESHDLMFTAYPDSIVIPGTDAQVN
                     LSFHGADAEHARLNIIVLLCILSIIGSFMLLYTRIFAQQIADPIFVMDKGLREWSYNL
                     EVRTLPGRDKEEVFRLAQVYNNRWLPLKSQIHSYRKTQGGETAEKSVLSLDGLL"
     gene            11025..>11808
                     /locus_tag="PKK76_02185"
     CDS             11025..>11808
                     /locus_tag="PKK76_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNL67299.1"
                     /translation="MIHPEGVFKQAWNALILVCTLFFAIEIPMRMALGYHIGGWLFAI
                     DVFITVCFMVDVVINFFTAEYVNGVLVTDKKVIAITYLKGWFLIDFLAAVPLDMVFVG
                     ALATLSDTARVFRLLRLARMARIAQFMRKLAQADIINISVLRMLFLTFWVLMVAHWSA
                     CVWIALGGTIGGVPDDEKNSLLTIYVRSLYWATTTITTIGYGDLTPGTTKQTIFTMLI
                     QLTGAGLYGYVIGNIASLIANLDVARAQYLEKLEKIGTYMKFK"
BASE COUNT         2945 a         2731 c         3205 g         2927 t
ORIGIN      
        1 ataaaccagc aataagcatt ttaccatatt gctcattttc actcactaat tctgaccgca
       61 ttcaatgcgt cattatgcca aatcttaagc acttcatgtc agggatccca ctatcggccg
      121 gagggcgttc ccctgcgcga ctgcgacgtg ttccctgttc cggtctcttc cgagggtttg
      181 gcttgtgttg gccctgctgc ggcatggcaa ggctcatcag ttctggaatc ggcggcgggt
      241 ccgggtctgt catgtgtatg tcggacgagg tgtgcatgtc aggttgtccg aggccgataa
      301 gagagtatgg ctcaggctct gaagttgcgc gtgcaatgtc acgcatgcgg atatatgatc
      361 gagggaactg cgaaatacgg agagggtcac tatgtgggcg agggggtgcc ttttgacttc
      421 gtggcaatcg ggaaggtgat caattccaag gggaaacggg tggtaaaggg agaggtcacg
      481 tgcatatgcc cgaaatgtac ggttagaaat aagtataatg tatgatgaaa attgggcagt
      541 catggaattg gcccattctt gctttggatc gttccggatg acggggtgtc cggttgcctg
      601 aagtggaatg tggtcgatcc ctgcccgcct tatgattttc ctggctctat atggccaaag
      661 cgaaacaacc tgctgaaatt cctgtaagca ccatgcgaca cagtgctgct catgtcctgg
      721 ctcaggctct acgggagaag tttccgaaag tggatctggc catcgggccg gatacggatc
      781 atggattttt ctatgacgtg atggtggatc cgccgctccg aagcgaagat ctgcctgatc
      841 ttgaaaagag aatgaagcga atcgtcaaag cgaatcagaa attcgaaaga ttctacctgc
      901 ccattgatga ggcgatcgag agattggaga aagaaggtca gggttataag gtggaaatgg
      961 cgcgcgacct caaggcccag ggcgaaactg agctgtcttt ctacgagaat actgtgaagc
     1021 agggcggtgc cgagaaatcg gcggtcttcg tagacatgtg caacggacct catgtggagc
     1081 ataccggaca gctaggcgcg ttcaaactgg tgagtgtgtc tggggcttac tggaaaggga
     1141 gaacagatca gcctcaactg caacggattg caggctactt gtttgccaca gaggaagaac
     1201 tgcgtactca tgaaaggaat gtggaggaag ccaagaagcg cgatcataga agactgggcg
     1261 tggatctaga gctctttact acatcagaga agattgggcc tggtttgatt ttgtggcttc
     1321 cacgcggtaa cgtgatcaaa gaagagctcg agggatgggc caaggacaca gaggagaaac
     1381 acggctatca gagagtcacg actccggtga ttaccaagga agatctcttc tataccagtg
     1441 aacaccttcc tcattacaag gagtctatgt ttcctccgat gagattggat gaagagaatt
     1501 actacatcaa gccgatgaac tgtccgtttc atcatacgat cttttccatg cgtccacgaa
     1561 gctatcgtga actacctctt cgcattgctg aatatggtct gtgtcacagg tatgaagact
     1621 ccggttccct catgggcctg atgcgcgttc ggggaatgtc tatgaacgat gcgcacatct
     1681 attgttcaga agatgacgcg gtggaggaat ttctcgaagt tctaaagctc catgagtact
     1741 actatcgcac tctggggata gaaaagtatc atatggtgct ggcgcttcgc aatccaaaga
     1801 acgacaagta ccacggcgat tcagaaatgt gggcgagagc cgaagctctg actcgtaagg
     1861 ctatggaaac gtccggaatc gcctatgagg tggagaacga tggcgctgcc ttctatgggc
     1921 caaaggtcga ttttcagata gtttcttcta taggaagaga gttcacagcg agcacttgcc
     1981 agctggatct tttcatgcct gaaaagtttg atctcaagtt cgtggacaaa gacggcagca
     2041 tgaaacggcc ggcctgtctt cacaggagtc cactgggcac gcatgagcgc tttgttggat
     2101 ttctcatcga gcatttcggc ggggcctttc cattgtggct agcgcccgag caggttcgga
     2161 ttctgccggt gagcgaaaac gtgcatgctt attgtcagga gctcgcctcc gcctgcaaaa
     2221 aggcagggct ccgagtcacg ctcgatgatt ccggagaaac tctggggaaa cgcattcgaa
     2281 acgctgagaa aatgaagatc cctcgcatgg tagtgatcgg agatgtagag aagcagaaaa
     2341 atgtgttgaa cagccgggat tatttctcgg gcgaaaagac agagatttcg ctcgatgcct
     2401 ttatcgcgga agcgctcgac gagattcgca caaaaaagct tagacagatc gcaaagccgc
     2461 tcctggttga tgcaggataa aaaattagct ggacaataaa ccggctttgg atttattcca
     2521 aatcaagaat tactctaaat gagtgatcgg aggactgaat gtcgaatctc aaaggctcaa
     2581 aaacacatga gaacctgaag gccgcatttg ccggggaatc ccaggcgaac aggcgttacc
     2641 tgtactttgc aaagcaagcg gacgtagaag gatatcctga tctggcaggt cttttccgcg
     2701 acacagcaga aggtgaaacc ggacacgcac acggtcacct ggaatacatg agaaaggttg
     2761 gggatccagc tactggcatg ccgattggcg acactgctct gaatctcaaa tcagcaatcg
     2821 cgggcgaaac gcatgaatac acggatatgt atcctggtat ggctaagaca gcgcgcgaag
     2881 aaggattcac tgaaatcgca gattggtttg aaactctcgc gaaagcagaa aagtctcacg
     2941 caggccgctt tgaacagggt ctgaaaactc tcgtataact gatcacacta agagcgcctg
     3001 tcctcaggac aggcgcagaa tttttccgga gtctttcatg aacgcgcgta ttaattatga
     3061 gaatacatct gatacggtgt atgatccagg caattctctc tactgggatg tggattcgac
     3121 ggatcgtgag ctggcgcgtg catttgatct atgcaatggc tgccggatgt gtttcaaata
     3181 ctgcccctct ttcccaactc tctttcaact gatggatgag aagcaggacg tcttcgcatt
     3241 gagtgcagag gacaaggaca agatcgtcgg ggaatgctat cagtgcaaaa tctgctatgt
     3301 caaatgtcct tacacagatc gtgatcatca taaattcaat ctggactatc ctgctctcct
     3361 acagcgtgcg gttcatgtaa aggcaaggaa ggaaggagtt ccatttcgcg acaagtttct
     3421 ggccaactct gattttgcag ggaagactgc gggtttactt tccggtcttg caaacaaagc
     3481 gatgaagagc aatttccatc gtcgcattat ggaagcaatt cttgggattc atcgcaacaa
     3541 acgaatgccg gagttccatt ccaccagctt catgcgctgg ttcaaaaaga agcccgcagt
     3601 tcctgttcag gccggccagg agaaagtagt tctcttttca acatgtttcg taaattacaa
     3661 caatccgcag cttggtcgtg acacagtaga agtgctcgag aagaatcaag tgcatgtgga
     3721 ttgcccggag caaaattgtt gcggtatgcc cggtttaaac agcggggatc tagactgggc
     3781 tatctcaaaa atgaagaaga atgtggaaag cctgtatcct tatgctaagg aagggtatcg
     3841 cattcttgca atcaacccta cttgctcgct taccttgaaa aaggaatacg taagctttct
     3901 tccgcctgag ctaaaagaaa aagcgcaggc agtggccagt gcgaccatgg atgtgcacga
     3961 gtatttgttt gagctcaaga agcaggagaa gctcaatcgc gatttcaaca gttcaccggg
     4021 caaggtgggc tatcatgtgc catgccattt acgcgcgcag aacattgggt acaggtcacg
     4081 ggatattctt cggctgattc cgggagctga aattgccgtt gtggatgaat gctgtggtca
     4141 caacggaacc tgggctatga aggaagaata ttttgaaatg agtcttgaag cagggaagcg
     4201 tgcatttgat gggctcaaag aaaaagaggc ctcacacatt gcaacggact gccccctggc
     4261 ggcgattcag attgagcagg gtatgggtct gggtgagaga ccggatcatc cagttcagat
     4321 tcttgcgcgt gcttatcgta aaccggagga cggaggtttc ccggatgcga tttcatctga
     4381 agagaatggt cagactggtt cgtaaacaag gagcgcacca tgaagaaact tgaaatggca
     4441 gacgtaattc cgaacacaga atacgagaag attcgggaca gctttcgttc aaagattctg
     4501 cgaatcaagg agccgcggcg catcgcagta ggaccgtact tgatgtttct ttttgaaaat
     4561 cgcgaaacca tgctttatca ggtgcaggaa atgatgcgag cagagggaat tcgcgcagaa
     4621 gaagcaatcc agcacgaaat caacacatac aacgaactgg taccgggacc ctgcgaattg
     4681 cgcgctactt tgatgattga atttgcagat gaggccacac gcagggtgaa gcttaaggaa
     4741 ctcgttggcc tggaaaagca cgttagcctg gtgattgacc gggattacgt gatttcagct
     4801 gtttttgatg agaaacagat ggatccggac aagttgtcga gcgtccagta cattcatttt
     4861 ccgctgggag aaaaagcggc ggaagctttc ctgtccacca atgatgtgga acttgttaca
     4921 actcatccgg cctgcagtta tcggcagagc atttcgcagg aacagctctc ttctctcaaa
     4981 gaagatctgt atacctgagg ctcgtagctt cgcgagcgtt gttgcatcga ttctgattct
     5041 gttcctgtcc ggttccatcc aggcagagga cgtagctgtg gccaggcgag cggtggttcg
     5101 cattgacatc agtttttttg agtattctta tgacactccc tatcgagcgc ctgcagtcag
     5161 acgtgcgagt ggaactggat ttatcattgc aggcaagcgc attctgacga atgctcatgt
     5221 ggtcagtcag tccaatacca tccgtcttca cagaccagac cagcgccagg actatccagc
     5281 acacattgta ttcattgcac atgattgcga tcttgcattg ctcgaagcgg atgatgatgc
     5341 tttctataaa gatacgcagc ccctggagat cggggtttcg ccggaactca attctccagt
     5401 cgtcgtagtt ggttttccga tcggagggga cagagtttcc atcacgagag gtgtagtttc
     5461 gcgcaaagac atggacacat atgcgcacag tactgtggat tcgcatgcga cgatccaggt
     5521 ggatgcggcg atcaatccag gaaactctgg cggtccggca cttcaaaatg ataaagtaat
     5581 cggtgttgca tttcagtctc taactcgcgg tgagaacctg gggtatttga ttcctccgga
     5641 ggtcattcga aagttcctga cggatgtgtc tgatggtcga tatgacggat acattgactt
     5701 tggcacaatg gacatgacta cgaagaatcc aattcttcga tctgcccttg gcctcgatgc
     5761 agacagtgtg gatacaggtg tgctcgtgta tgatgtgctg cagggaagtt ccgcggatgg
     5821 ctttatagaa cgcggtgacg ttttgctgca ggtgaatggc aagaagctat ccttcgaggg
     5881 agaggtggac tggaacggag gtctgcaccc gtacattgag atgcttgata atcttgcatc
     5941 cggaaccatc attgaagtgg aacttctgag aaaagggaag aaggtaactg taaaattccc
     6001 tgccaggaaa tcgaaggtgg tagatgcact gcgaatcaac tatgataccg ctccgcctta
     6061 ctggatcgaa gcgggtcatg tgtttcagcc tctggatgca aacctgatgc agcaaaattc
     6121 gcgaaactgg gcgcgcgaag gaagggcgga tcttacatat tactacaaca actttctgag
     6181 tgcaggcatt tatcgatctg tagatggctt tgtgatgctg actcaacgtc tggcggatcc
     6241 ttccaacatt tataacgatc gttttgtttc gcaggtcgta gagtctgtga atggagagaa
     6301 agtaaaaagt ttcacgcatt tctcatcgct tatggccgcg gctcacaaag agcctaagat
     6361 tacgattcgt tttcgtgact ctgcagttcc attggttctc gagacggctt ctctttcgca
     6421 aacaaacgaa aggattcgca aagcgtacgg acttacatcg ttgaattttg ttcccggaga
     6481 aaagaaatga ttcgcagcct gttttctctg tttcttctga ttccgggtgt ttctttgcca
     6541 gacgcggacg ttcgccgggg cattgtcctg cttacgatca ctcctgttgt ggaagatgcc
     6601 accagaccct ggatcaaaga ccagggcact ccatttcaga ggctgggact ggtcgttgga
     6661 aaaggcaaga tccttgccct tgaaagcgaa gtgcgcggag cggcgattgt agaagtgcga
     6721 aagcattctt ccacggaagc ggtgactgcg acgattgaac gttcggatcg cgaaacggat
     6781 ttagccattc tctctgttcc cgctgacttt gcgcaggatt tgaatccaat tcctgccggg
     6841 ccagatcctg tggtgggtga aacgcttgag tcgattcgtg tggacggagg tttgtttcgt
     6901 cttcagagaa atgaagttca gttttcagaa atcgaagtga ccaccgctac agggttcact
     6961 gccttccctg taggcaacgt ggcatcgctt gagccttttc ctgaaggcgg aattctgatt
     7021 tcgaaaggca agctggcagg ttttgttcat gatcgatcca acggcaggcg gattgacttt
     7081 ctattgccgg gatacattca ccagtttctg gccgggggtg cgccaggttc agcaggcttt
     7141 gactacgaat ctctggtgga tcctgtactc agacgcaacg caggtgtgga agggagaagt
     7201 ggaccaatgg tcactcgcat ctacccagga acgagcgcct acggtgtgtt acagaaagga
     7261 gacatcattc ttgccgtcga cggagtcgtg ctggatgaga ggggcttttt cgaagacgga
     7321 aagaacggaa ggcagagaat cgaacttctg ttgtacgcga aaggtggcgt gactcgcaaa
     7381 attggagata ctgtgcccat tcggatcatt cgcgcggcca ggcgaatgga cgtatcgttt
     7441 aaattgattc gaaatgaggg aaatgcggtg cgggtgccga tccgcgaaag agagcagggc
     7501 tttcttgtgg aaggcggcgt agtgttcttg aatttgtctc tgtttttttt gcaaagccag
     7561 tttggagcaa cgtatgccac ttctgcgcct gagctcgcct atctttacca gagcaaacgc
     7621 ttccgggatc agccaggaga tgatcgcatt gtaatcattg cgcgcgttct gccggatgat
     7681 atgaccctgg gctatgaatc tctcggagga gcgcaagttc ttcgcgctgg aaatgaaaca
     7741 gtgaagagcg tcaatcacct tcatgacatc atggaagcgg ccgcgaagaa ggacgctacg
     7801 ctcactcttg aattatccaa tggaagaatg atggttctgg atttgaaaaa tcgaaacgca
     7861 atcaacagta gaatcagaaa acgatacgga gtgcccgtag actttagacg tgcttctaat
     7921 tctggtcctg gaggttgaag ccgttattgt agaaagtaac gcaggccaat gtttgcgcgc
     7981 actttgtcgc ggtatgcatc ttctgctccc aggtgccgaa tcgggaagtc aagtcctgct
     8041 ctgagtgcca gggattttcc ggagagcatg aggccggggg agaaaaaaac agttccgtat
     8101 tcagttccag gcagatcttc gcctgcgaaa cgatccgcgc cataaaactg cgaagtgacg
     8161 ccaagaacca ggttcacggg ccatgcctca ttgttgtagg cacccagacc aaatcccatc
     8221 gacaggccac cggatctgat atctcctgga cgaaagtccc ctctggtgcg atttgcaatt
     8281 tgaccgccct tcaattcgaa gctgagcctt tcaatgcgat attgcagact catggcagag
     8341 tcaaatctgt atccatctgc caggcgcgac tcgggcggga gactggagga acccctggct
     8401 gtagatactc ccgcgatctc cataaaagtc agtctgaggt ttctggaatc gatggcgcga
     8461 aagcggcctt ccatattcac gaaattcagc ccctctgaag atgcctcagt ggcgccttcc
     8521 atctcgcgtc tggcgtactc cagggagcca gtgaggtaga accttcccag ggtggcttcg
     8581 agtggcagga aaacgggtac ggtgtaggcg cgaatggact ggtctgatgt gcggcgctca
     8641 tagtaaccca tacccgctcc taccatcccc gtgaggggag ctggctgagc gagagcaggc
     8701 gatgaagcga gcatcaggat tagtaaggtt tcacgtattc gcacgactaa taggttagac
     8761 ggtcagattt ttttgattgc gcccgatttc tatgcttttc aagtaacttt tgcctattgc
     8821 gcgaaaaatt gccatcacaa accgatagta tagtatcgtc gttccatgag tgaagaaaaa
     8881 aagataatgt cggaattcgt gggcaagctc tgggcaaatc ctgccctggc aggtgcctcg
     8941 gtcgtccgcc gcgaaaacca gatcctcagc ttcattcggg aaaaccagac gcagctgcaa
     9001 caggcattcg ccaaaccggg ttatttcccg gatctgggct gggacggctc tatccggctt
     9061 ctgttttctg tactcacgga tttaatcgta gattccgtag aaccacgcct ggacaagatc
     9121 ctgggcgctc ttttgaaccc ggaagtattg ccgcagctgg aaatcagacc ccaggaagtg
     9181 gaccgcgaac gcatcaagga ttttatactc aaaggtcttc gaaacaaagc aactcgcgac
     9241 ccatacattg ccgtgctgga aacgatttcc tacggcctct atgatcgcta cgtacccaaa
     9301 gcactcgcaa ggcacaaagt gatccacaat gaattctata gaaaggatcg attgagtctt
     9361 ccggcgccca tcatgattgc ctatctgcaa ctgtgctctc tgtttcgtcc cctctactgg
     9421 cagaaagtgg ccacagcgtc taacaatcag ttgaacctgg cggccgttgc gaaagatccc
     9481 aggatgttcg attccactta taaattcctc aatgcgtttc tggtgcaaga gatgggcgga
     9541 gttccggatc gaattctgcg ggtggggctc gaaagctgca tgaatgcaga agagcatccg
     9601 gacaccggtg ggtcctcgcg gctgatcggg attctagtgg cacgcgcagc tgaatttgat
     9661 ccgacccaga aacaggaccg cggcgcggaa agtcccgaca aatcctggtt tcatattcat
     9721 aagaaaacag caaagtatta cggatacgac gcacgctatc tggacgaact ctatcagatt
     9781 gcaagcgaag aggcgtggtg agtatgagca gctggaagaa ggcatacaat ccggatcacc
     9841 ttcctggttt catcgagacc ggcattatct tactgatcat cctggtgatc gggcacacaa
     9901 ttacggaaga cattgcagtg atttatcact gggaccatcg atggatgttc tatctgacag
     9961 ttgctggatt tgtatttgat ttgattttca ccgctgagtt tgcagcacgt tctatcatca
    10021 ctctgaagca tggtcatttc aagcactacg taacgcaaga gagggggtgg atcgatgccc
    10081 tcacttcttt tccgcttctt ctgcttgttt cgggtccggc cctggtggtt tacctgacgg
    10141 atgcgcatgc ggaaggtgct ttctttagtt tcttgatcat cctgaaaact gcgaaagcga
    10201 ttcgcgtaac acgcgtgctg cgattgattc gggtgatcaa gatttttggt aagattcaga
    10261 atacagaatc tcaaatgacc aatcgccatg ttgcgacgat ttctaccatt gctgttgttt
    10321 ctctcgtcag tgttctggtg attgcccagt tcgtgccgtt cttgcatata ggggatcatg
    10381 aggcctatcg ccagcgtcgt gtatccgaat tgtcttctct ctttcaggca gccagtgcgt
    10441 tcccggaagg tgtggataga agcagtggga ttcaaaagat gattcgcgcg gaaggcaagg
    10501 gagacgtgat caagatcaag cgcgcaaacg gtgaagtggt gttcgaagcg gatcccggcg
    10561 aaagccatga tttgatgttc accgcctatc cggattcgat tgtcattcca ggtacggacg
    10621 ctcaggtaaa tctttctttt cacggcgcag acgcagagca tgcgcgcctg aatatcatcg
    10681 ttcttttgtg catcctttcc atcattgggt ctttcatgct gctgtacact cgcatcttcg
    10741 ctcagcaaat tgcagatccg atcttcgtca tggacaaagg ccttcgcgag tggagctaca
    10801 acctggaggt tcggactttg cctggacgag ataaagaaga agtcttccga ctggctcagg
    10861 tatacaacaa ccgctggttg ccgcttaaga gccagattca ttcttataga aagacccagg
    10921 gaggcgaaac agccgagaaa tcggtactct ctctggatgg tctgctctaa aacctcagag
    10981 ccctttcaag aacgcacccg gattgcccga ttttaatagc gggtatgatc catccagagg
    11041 gagtatttaa gcaggcctgg aatgcgctca ttctggtgtg cacgctgttt tttgccatcg
    11101 agatcccgat gcgtatggcg ctcggctatc atattggtgg ctggctcttt gccattgatg
    11161 ttttcatcac agtctgcttc atggtcgacg tcgtaatcaa tttcttcacg gcggaatacg
    11221 tcaatggtgt cctggttaca gataagaaag tcatcgccat cacctacttg aagggatggt
    11281 ttttgatcga ctttctcgcc gcggttcctc tggacatggt ctttgtggga gcccttgcaa
    11341 ctctttcgga cacagcgaga gtatttcgtc tgcttcgcct cgcacgcatg gctcgtatcg
    11401 cacagttcat gcgaaaactg gcccaggcag atatcatcaa tatcagcgtc ttgcgaatgc
    11461 ttttcttgac gttctgggtt ctcatggtgg cgcactggtc tgcctgtgtg tggattgctc
    11521 tgggaggaac gataggcggc gttccagacg atgagaagaa ttcgcttctt acgatctatg
    11581 ttcgctctct ttactgggcg accacaacga tcaccaccat tggatatggc gatctgacgc
    11641 ctgggactac aaagcagact atcttcacca tgctcattca gctcacaggt gcgggccttt
    11701 acggttacgt cattggtaac atcgccagct tgattgcgaa cctggacgta gctcgtgctc
    11761 aatacctgga gaagcttgag aagattggga cgtacatgaa gtttaaga
//
LOCUS       DAORFF010000028        11668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2446_length_11668_cov_3.765608, whole genome shotgun sequence.
ACCESSION   DAORFF010000028 DAORFF010000000
VERSION     DAORFF010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2446_length_11668_cov_3.765608"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="PKK76_02190"
     CDS             <1..960
                     /locus_tag="PKK76_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNL67300.1"
                     /translation="AAMVDLTNPDARKWIKEVIKAELIAGGSSGWMADFGEALPFDAK
                     LFSGEPASSYHNRYPEEWAMINREAIQEAGKTDIVFFCRSGYTRSPSQATLFWLGDQL
                     VSWDEYDGIKSSVTGLLSGGISGFTLNHSDIGGYTTVNNPIKNYHRSKELFMRWTEMN
                     AFTTVFRSHEGNRPAENHQFNSDAETLQHFARFAKIYAAWFDYRKKLMEEAAQHGWPV
                     IRHLYLEFPDDPAVRSIRYEEFMVGSEILVAPVLDPSAKSVSVYLPRGEWVHLWTGDI
                     KGSADHGSRLTVDAPIGKPAVFYKKGSAVGEAFRKDLATRELL"
     gene            960..1352
                     /locus_tag="PKK76_02195"
     CDS             960..1352
                     /locus_tag="PKK76_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67301.1"
                     /translation="MEKLTPIQTIQLVLGGLLIAAGVFLGYKTFTKANAILDQPAQLS
                     GWLELRASIHPPAKQEEKSSLLDFKPQMSLIRDEQVYVLGGYLTLFVAAFFLLVAARI
                     AAAFLTAGSKILSGVFEKRRESENQNQN"
     gene            complement(1354..3066)
                     /gene="sulP"
                     /locus_tag="PKK76_02200"
     CDS             complement(1354..3066)
                     /gene="sulP"
                     /locus_tag="PKK76_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HNL67302.1"
                     /translation="MLKPKLFTLLREMTPAQLLSDLTAGVIVGVVALPLAIAFAIASG
                     VSPERGLYTAIIGGFIISFLGGSRVQIGGPTGAFVVIVYAIVEHYGYPGLAAATLMAG
                     VILIIMGLARFGSAIKFIPHPVIVGFTSGIAVIIFSAQIRDVLGLSVEKVPADFIEKW
                     VEYVRHISSINVHAAAISGGCLLVLILWPLLTKRIPASLVALVGSTAIVYFLNLPVET
                     IGSRFGSIPSTLPAPSLPEISFQNVRNLIQPAFTIAMLGGIEALLSAVVADGMIGSKH
                     RSNMELVANGVANVLSPLFGGIPATGAIARTATNIKNGARTPVAGIIHAITLLLIMLL
                     LAPLARLIPMACLGAILIVVAYNMSEWRTFVALLKTPKSDVIVLLITFGLTVIVDLTV
                     AIEVGLVLAAFLFMNRMAHVTNIAQFEKEFEDSDDLDDPLSIHKRQVPDDVRVFEING
                     PFFFGAAYKFEEALQVMDRYPRVIIIRMRNVPAIDSTGMHVLKQVHRQAQSRGTQMVL
                     SGVHTQPLFAMEKAGMLTLFGEDNIFGNIDDALNRAREVLGLPRVERPGEFVPTVARE
                     GRPS"
     gene            3175..4080
                     /locus_tag="PKK76_02205"
     CDS             3175..4080
                     /locus_tag="PKK76_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNL67303.1"
                     /translation="MSEIVSVINYKGGVGKTTLTLQIGVGLVTLFQKRILLVDLDPQC
                     SLSLSTVDEHYWADRVEKQGSVREMIQSFYESEKPECNPDWIIENALDRAWDSMPGKL
                     EGLDLLPGHLDLPDYEMKLVAKKPGHMNAEEYQLKRYMILKDALAKVRKKYDMILCDC
                     PPNIYMVARNAIIASDYFLVPTIPDFISCYGVPFILEHIKAMQEEVRERGGHTEAKFL
                     GIVRNRVRQAGSHLVKEHEEHSVKLRERYEGALFDSMIIDRIGVAELLGSRENLYTAK
                     SERSNEIKKDFTHLVREVLRRMGKD"
     gene            complement(4100..4489)
                     /locus_tag="PKK76_02210"
     CDS             complement(4100..4489)
                     /locus_tag="PKK76_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNL67304.1"
                     /translation="MQIHHIAITAREVPLVASFYETVLGLRRIREQSDSSGLRSVWLD
                     LAGAILMIERADRTAAPVGSFHERPPGYYLLAFRIAKEERESYIAKFAEKRVPVVYST
                     DFTIYILDPERNRIGLSFYEMDLDGAR"
     gene            complement(4490..5068)
                     /locus_tag="PKK76_02215"
     CDS             complement(4490..5068)
                     /locus_tag="PKK76_02215"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNL67305.1"
                     /translation="MTASEILERLRSQDLVPLRLPSHYRQAAVAVILRESRDGLRMLF
                     IQRTAHPGDPWSGHMAFPGGRVDPEDESPLAAARRETMEEVGIFLEDRHYVAPVQEIQ
                     ASARGSRMELIVFPFVFYWDGDSELIHAADEVAATVWVPVDLVLDQTRHTTFMNQYED
                     KTVQMNCIEYEGYRIWGMTYRMIQNLISAVNA"
     gene            complement(5068..5616)
                     /locus_tag="PKK76_02220"
     CDS             complement(5068..5616)
                     /locus_tag="PKK76_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67306.1"
                     /translation="MRSSGSHRLFRFLSSVAFVLPLVAGFSQCRPPIRGEFGFSPVDR
                     RDSDALEKTLLTETEFRLGRENLYFYDHETIWWIYRMEEGPYSQGDFLVSLYENNLTP
                     EPVEVDMRHVDVMKQGSIGIIQQRYEPLRPGDYILKIAQHARVVDEARFRVLPGEPEV
                     EDEDSVSDESDDLRKYARRADS"
     gene            complement(5632..7221)
                     /locus_tag="PKK76_02225"
     CDS             complement(5632..7221)
                     /locus_tag="PKK76_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67307.1"
                     /translation="MRMRALAILALLLGACAPYQTRNDFQSARRDVSRGGPQASKIAN
                     DILANLNDIPPAMVGEALDTLIAIPDPEGERAVLEASTRPQFQAPEYREKILRRLLGS
                     DSQQARDFVLKEIKAKPDLMNDPTILSWVVAKDPPGASELVLAHVQKNPASLNEQTIN
                     YFGTKRYTPSIPVLLSSIDSGKNADLCLKTLAQMGDPKADAAILSIAQKESPVQMRAI
                     QALPEMKDPDRRKEAESVLEGMWKNNPPGEMKTTALTGLAKMRGQDPLELEREAAFPM
                     ANVQGDLAVNSLEDRAAKRDMISPEPVKPAVTPVQPVVTQPTVVPTRPVQPSQPQVVQ
                     PAQPSKPVVTAPRNEPAHTQPAKPDAKKDQVAVRTEDKTKPADKKDEKRDDKKIAATD
                     NKPRKPDPKDEPPPLDQRASDAYRERISNLFADTFGDGGSQVLRRIQDSLFTYSESNS
                     TAAEFMVRSYRKRYGGSDTAIRKMMARGLKQPDCMAAILNNIKTEYPTKEMQIYAISK
                     LFALQRWQSQVVLELSQETQL"
     gene            complement(7275..7475)
                     /locus_tag="PKK76_02230"
     CDS             complement(7275..7475)
                     /locus_tag="PKK76_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67308.1"
                     /translation="MKLIFLALVITILYQMSTQAMARVNSRASGEGNQQKSPGERKPL
                     LSREKKPTVEKDITHRGRVLRD"
     gene            complement(7502..9193)
                     /locus_tag="PKK76_02235"
     CDS             complement(7502..9193)
                     /locus_tag="PKK76_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNL67309.1"
                     /translation="MNFKKTVFSLLLISLSLGAVEAKRFAIVMGSNYTGNSAGIPELE
                     LCESDAKLMEQTLKSNGSYDDATVLLGPMVTSSRVKSTLEAIAQKAGKEDVVAIYFSG
                     HGTYQRDASAPNGLRNYIVMYDRPHVSDKELSDWIKGIKTQHVTLIMDCCYSGGIAKK
                     GRRGVGDIPTSPGSDGTVIQNGDENFYFQNKAIIASSDSNETSIEVRGSINHGVFTYW
                     FAQGLDPRNGDLNKDGTVTALEAFEWSSRRVADMAKKQFNHDQNPQISGNAKGIFLAG
                     REVPTPQPPVVTPVVNPPQPDPNKPQPPQPPQPPVVTPVTPVTPTPPNVEPPPENPNT
                     NDTGKVIIATTILRSQLSGTQTPDPAMMMRRMRGVDSDRTVKVRFSGKELPATVTWVD
                     QAGLKAATGEDIPLGTYTVGRQGTPTVVPNTVALIEVNGVPGGVHEVEIISEDYPVIK
                     ETIGVEGKNVVKKFVVASLRGTGSIQGRVFLKNFEQPLPGQTIWMPTVVGTNQVHKMK
                     SLTDGSFWFMNLPPGNYYEIKASFEENLKLDNQYIKVKDGDVTKVDIVLSQKNFK"
     gene            complement(9227..9469)
                     /locus_tag="PKK76_02240"
     CDS             complement(9227..9469)
                     /locus_tag="PKK76_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67310.1"
                     /translation="MLEEGRVDRFLDLSESLRSQKPITTSPKTQEEAELLAACQDVLD
                     GMVPLMERALAQISRDEFETRVSGQIQEFLAKKEEP"
     gene            complement(9510..9941)
                     /gene="fliS"
                     /locus_tag="PKK76_02245"
     CDS             complement(9510..9941)
                     /gene="fliS"
                     /locus_tag="PKK76_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNL67311.1"
                     /translation="MMAAKKTHEAYKDTEIQTAGQGKLIVMLYEGAIRFINVAMSNMT
                     PRKYDVVNNNIIRAQDIVTELMLSLNMEEGGEIAQNLFNIYAYIKKRLLEGNVQKDNE
                     VLTEVVKLLEQLRDAWDQASRQSPPRVSVPAREGASFSITG"
     gene            complement(10070..11404)
                     /locus_tag="PKK76_02250"
     CDS             complement(10070..11404)
                     /locus_tag="PKK76_02250"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL67312.1"
                     /translation="MAEFRMFSRMRSLFNPASSVYFYGLAVNSSTILLIRFVDPVYGP
                     RIEWIPFIAFVSYYIFIALWLSRRLVRGARSLESWFDLVLITSFPVAIYYGYLSFVLP
                     PYSIVFWIWGTAVGMPGFGYIRRRYWIVWVLIMFAATIPGFVLGAFEIRIILSMAWVV
                     IMTFYMTRASNQLKKSRVALSVHLTEVRRNKRDLEKAHQKLKTAADRFQVELELAERL
                     QQGLLPTLGTLSSGLTLRGRYLAMQNLGGDFYDVATAGDKTVLLVADVTGHGVAASLI
                     TTMTKASFHAHKHLLDPSEILAAINSDLCEFLTGGQHFVTAVCVQFDVSARRVIHANA
                     GHPPGFHLSDGRLTTLGDDSLLLGLDRSLKWKSYTIEAKKSDRFFFYTDGLTETFSED
                     RSMYGERLESMLIKHSSLDSEAFLAALFDDLLEFRGSSPQSDDIAVVCVDMP"
     gene            complement(11427..>11668)
                     /locus_tag="PKK76_02255"
     CDS             complement(11427..>11668)
                     /locus_tag="PKK76_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNL67313.1"
                     /translation="GTEVKILRAGEMFGEYGSLGDSVRNATVTALRDSVVLEIARGEI
                     RKIVESAPIFNFSMREIMIRRSRELKKLEKSGLTT"
BASE COUNT         2917 a         3019 c         2910 g         2822 t
ORIGIN      
        1 gcagccatgg tggacttaac caatccggac gcgcgtaaat ggatcaaaga agtaatcaaa
       61 gcggaactca tcgcaggtgg ttcgtccgga tggatggcag attttggcga ggcacttcca
      121 tttgatgcga agctcttttc cggggaaccg gcttcctcgt atcacaatcg ctatccggaa
      181 gaatgggcca tgatcaaccg cgaggccatt caagaggcag gaaaaactga cattgtcttc
      241 ttttgtcgct ccggctatac tcgcagccca tctcaagcca ctctgttctg gctgggtgac
      301 caactggtaa gctgggatga gtatgacgga atcaaatcct ccgtaacagg tttgctcagc
      361 ggtgggatat ctggtttcac tctgaaccat tcggacatcg gcggttacac cacggtcaat
      421 aacccgatca agaactacca tcgctccaaa gaactattca tgagatggac agaaatgaat
      481 gcgtttacta cggtctttcg ctctcacgaa ggcaatcgac ctgcagaaaa tcatcagttc
      541 aattcggatg cagaaacttt gcagcacttt gcccggttcg cgaagattta cgctgcctgg
      601 tttgactacc gcaagaaatt aatggaagaa gcagcgcagc atggatggcc cgttatacga
      661 catttatatc ttgaattccc ggatgatccc gctgtcagat ccatacgcta tgaagaattc
      721 atggtgggct ctgaaattct ggtggctcct gtactggatc cttctgcgaa atcagtttcg
      781 gtgtatttgc cgcgtggaga gtgggttcat ctctggacag gagatataaa agggtctgca
      841 gatcacggaa gtcgcctgac ggtagacgcg ccgatcggaa agcccgcggt gttctataaa
      901 aaaggctcag ccgtaggaga agcgttcaga aaagacctgg ccacaaggga gctgctttag
      961 tggaaaagtt aacacccata cagacaattc aactggtcct gggcggccta ttgatcgcag
     1021 cgggcgtctt tcttggctac aaaacgttta caaaggcaaa tgcaatcctc gatcaacccg
     1081 ctcaactctc tggctggctg gagcttcgcg cttccatcca tccacctgca aaacaagaag
     1141 agaaatcttc cctgctcgac ttcaagcctc agatgtccct gatcagagac gagcaagtct
     1201 acgtgctcgg gggatatctg acgctgtttg tagcggcctt ctttctcctg gttgcggcca
     1261 ggatagcggc cgcattctta actgcaggct cgaaaattct ttcaggagtt tttgagaaac
     1321 gacgcgaaag cgaaaatcaa aaccagaact gaactacgag ggccggcctt cgcgggccac
     1381 cgtaggaaca aattctcctg gtcgttctac cctgggaagt cccagaacct ctctggcccg
     1441 gttcaacgca tcatcgatat ttccgaaaat attatcttcg ccgaataacg tcaacattcc
     1501 cgccttttcc atggcgaata acggctgagt atggacacca gacaaaacca tctgcgttcc
     1561 tctgctttgc gcctgcctat gcacctgctt gagtacatgc attcccgtag agtcaatggc
     1621 cggcacgttc ctcatgcgaa tgatgatcac ccgcggatag cgatccatca cttgtagcgc
     1681 ttcttcaaac ttataagcgg ctccaaaaaa gaaagggcca ttgatttcaa aaacacgtac
     1741 gtcgtccggt acctgccttt tgtgaatcga aagaggatca tcgagatcat ctgagtcttc
     1801 aaattccttt tcaaactgtg caatgtttgt tacatgcgcc atgcgattca tgaacagaaa
     1861 cgccgcgaga accagaccca cttcaatggc cacagtcaga tccacgatca cggtcaatcc
     1921 aaatgtaatg agaagaacaa tgacatcgct ctttggagtt ttcaacagtg ctacaaaggt
     1981 tctccattcg ctcatgttgt aagcgactac aatcaggatc gctccgaggc acgccattgg
     2041 tatcaatcgc gcaagaggtg cgagaagaag catgatcaga agaagtgtaa tggcgtgaat
     2101 gattccagca acgggagtgc gcgctccatt cttgatgttc gtcgcggtgc gcgcgatggc
     2161 tcctgttgcc gggattccgc caaacagagg cgaaagaaca ttggccactc cgtttgctac
     2221 cagttccatg ttggatctgt gtttgcttcc gatcattcca tccgcaacga ctgcagacag
     2281 caaagcttca atccctccca gcatcgcaat ggtgaatgca ggctgaatca agttgcgcac
     2341 gttctgaaaa gaaatctctg gcagagaggg agcgggcaac gtggatggga tagatccgaa
     2401 tctgcttcca atcgtttcca ccggaaggtt tagaaaatat acaattgctg tagacccaac
     2461 gagtgcgacc agagatgccg gaatgcgctt tgtcaaaagc ggccaaagaa tcagaacaag
     2521 aagacatcca cctgaaatgg cggcggcgtg aacattgatg gaactgatgt gacgcacgta
     2581 ctcgacccat ttttcgataa aatccgcagg cactttttcc acagacaagc ccagcacgtc
     2641 gcgaatctgt gcggaaaaaa tgatcaccgc gattccgctt gtaaacccta cgatcactgg
     2701 atgaggaatg aacttgatgg cggagccaaa gcgcgcgagg cccatgatga tcagaatcac
     2761 cccggccatc agagtggcgg ccgcaaggcc cgggtatccg tagtgttcca cgatcgcgta
     2821 tacgatcact acaaaggcac cggtgggacc gccgatctgt acccggcttc cacccaggaa
     2881 ggaaatgatg aaccctccta tgatcgcagt atagaggcct ctttctggcg acactccaga
     2941 cgcaatggcg aaagcgatgg cgagaggaag agccactact ccgacaatca cgcctgccgt
     3001 tagatcgctc agtaactggg ccggtgtcat ctcgcgaagg agtgtaaaga gctttggctt
     3061 gagcattcgc cctggattat accgccgcaa aaacggattt caagcagaaa tcccgcgtcg
     3121 cacaggcgga aggcagactt tttccggcag aacggccgat aatatagcca tcagatgtcc
     3181 gagatagttt ccgtaatcaa ttacaagggc ggagttggta agacgacgct gacgctccag
     3241 attggagtgg ggctggtcac gctcttccag aagcgcattt tgctcgtgga tctagatcca
     3301 caatgcagcc tgtctctctc caccgtggac gaacactact gggcagatcg cgtggagaaa
     3361 cagggctccg tacgcgaaat gatccagtca ttctacgaat cagaaaagcc agaatgcaac
     3421 ccggattgga tcatcgaaaa cgcactggac cgcgcctggg atagcatgcc cggaaaactg
     3481 gaggggctgg atctacttcc gggtcacctc gatttgccag actatgaaat gaaactcgtg
     3541 gcaaaaaagc caggacacat gaacgcggaa gaataccagc tcaaacgcta catgattctc
     3601 aaagacgcgc tggcaaaagt cagaaaaaaa tatgacatga tcctgtgcga ctgtccgccc
     3661 aacatctaca tggttgcgcg aaatgcaatc atcgccagtg actacttcct cgttcctaca
     3721 attccggatt ttatctcatg ctacggtgtt cctttcattc tagagcacat caaggcaatg
     3781 caggaagaag tgagagagag aggcggccat acagaggcga aattcctggg aattgtgaga
     3841 aatcgggtgc ggcaggcagg ctctcatctg gtaaaagagc atgaagagca ttctgtgaaa
     3901 ctccgcgaaa gatatgaagg agccctcttc gattccatga tcattgatcg cattggtgtg
     3961 gcggaacttc ttggcagccg cgagaatctc tatactgcga agtcagaaag gtccaacgaa
     4021 atcaagaagg atttcacgca cctggtacgc gaagttcttc gtcgcatggg aaaagattaa
     4081 caaaaccagg cctctcgact cagcgagccc cgtccaaatc catttcataa aaagaaagac
     4141 caatccggtt cctctcggga tccaggatgt aaattgtaaa gtctgtcgag taaacaacag
     4201 gcactctctt ttctgcgaac ttcgcgatgt atgattccct ttcttctttt gcaatgcgaa
     4261 atgcaaggag atagtagccc ggcggacgct cgtgaaagct tccaacaggt gcagccgttc
     4321 tatccgcgcg ttcgatcatg agaatcgcgc ccgcaagatc taaccagacg gatcgtaggc
     4381 cagatgaatc tgattgctca cgaattcgac gcaagcccaa caccgtttca taaaaagaag
     4441 caaccagggg tacttcgcgc gcagtgatcg cgatatgatg aatctgcatc tatgcgttca
     4501 ccgccgaaat cagattttga atcatgcgat acgtcattcc ccagattctg tatccttcgt
     4561 attcaataca attcatctga actgtcttgt cttcatactg attcataaaa gtagtgtgcc
     4621 gtgtttgatc cagtactaaa tccacgggaa cccagactgt ggctgccacc tcatctgcgg
     4681 cgtgaatcaa ttccgaatcg ccatcccagt aaaagacaaa tggaaatacg atgagctcca
     4741 ttctgctccc tcgcgcagat gcctggatct cctgcactgg agccacatag tgacgatctt
     4801 ccagaaaaat gcccacttct tccatggttt cgcgccgggc cgcagccagt ggcgattcat
     4861 cttccggatc cactcgacca cctggaaagg ccatgtggcc agaccatgga tctcctggat
     4921 gcgcggttct ctggatgaaa agcattcgca gaccgtcgcg gctttcgcgt aaaatcacgg
     4981 ccacggccgc ctgacggtag tgagatggta gtcttaaagg aaccaggtcc tgcgagcgca
     5041 gacgctcgag gatctcagaa gctgtcatca ggagtccgcg cgacgagcat acttgcgcaa
     5101 gtcatcggat tcatccgaaa cggaatcctc gtcttctact tctggttctc ctgggagtac
     5161 gcgaaacctt gcttcgtcta caacacgagc gtgttgtgcg attttcagaa tgtaatcccc
     5221 tgggcgaaga ggctcataac gttgttgaat gattccaata gaaccctgct tcattacgtc
     5281 cacgtgacgc atatccactt ccacaggttc gggcgtaaga ttattttcgt acagagaaac
     5341 aaggaaatcg ccctgcgaat aagggccttc ctccatccgg taaatccacc agatggtctc
     5401 gtgatcgtag aaatacaaat tctcacgccc caggcgaaac tcagtttctg taagaagagt
     5461 tttctcaaga gcatccgagt ctcgtctgtc tacaggagaa aagccaaact caccgcgaat
     5521 cggtgggcga cattgcgaaa aaccagcaac taaaggcaga acaaaagcca cgctggacaa
     5581 aaagcgaaaa agcctgtgtg aaccagaaga acgcatagct tagcctccta cctacagttg
     5641 cgtttcctgg ctcaattcca ggaccacctg ggattgccat ctctgcaaag cgaatagttt
     5701 agaaattgca tatatttgca tttctttagt aggatattca gtcttgatat tgttaagaat
     5761 agcagccatg cagtctggtt gtttcaggcc gcgtgccatc atcttgcgaa tggcagtatc
     5821 acttccgccg tatcttttgc ggtaagatcg caccataaat tcagctgcag tgctattgga
     5881 ctctgaataa gtgaacaatg aatcctgaat tcttcgcagc acctgactgc cgccgtctcc
     5941 aaacgtatcc gcaaagagat ttgagattct ctcacgatac gcatccgatg ctctctggtc
     6001 gagcggaggt ggctcatctt ttggatctgg tttgcgaggt ttgttgtctg tagctgcgat
     6061 cttcttatcg tctcgctttt cgtctttctt gtctgctggc tttgtcttgt cttccgtgcg
     6121 aacagcgacc tgatctttct ttgcgtccgg ctttgcaggc tgcgtatgcg caggctcatt
     6181 gcgcggagca gttaccaccg gttttgaagg ttgagctggc tgaacgacct gcggctgcga
     6241 gggttgaacg ggtcttgttg gaacgactgt gggttgagtg accacaggtt gcaccggtgt
     6301 tacggccggc ttcactggtt cgggagatat catatcgcgc tttgccgcgc gatcttcgag
     6361 agaattcact gcaaggtcac cctgcacgtt ggccataggg aaggcagctt ccctttccaa
     6421 ttccagtggg tcctggcctc tcatcttcgc aagtccagtc aatgcagtgg ttttcatttc
     6481 tccgggcgga ttatttttcc acattccctc gagaacgctt tccgcctctt ttctgcgatc
     6541 tggatctttc atttctggca acgcctgaat ggcgcgcatt tgcactgggc tttctttttg
     6601 tgcgatagac aaaatggccg cgtccgcttt tgggtctccc atttgagcca gagtcttcag
     6661 acagagatcc gcattctttc cactgtcaat ggaggaaagc aacaccggta tagatggcgt
     6721 ataacgtttc gttccgaaat agttgatggt ctgttcattc aaggaagctg gatttttttg
     6781 tacgtgagcc agcacgagtt cagatgcacc cggaggatct ttcgcaacca cccaggagag
     6841 gatggttgga tcattcatca gatccggctt tgccttgatt tctttcagca cgaagtcgcg
     6901 cgcctgctga gaatcggaac ccagaagtct gcgcagtatc ttctcgcgat actccggcgc
     6961 ctggaactgc ggccgggtgg aagcttccag tacggcgcgc tcaccttccg gatctggaat
     7021 agcgatgaga gtatcgagcg cctctcctac catggctgga ggtatgtcgt ttagattcgc
     7081 cagaatatca ttggcgatct tgctcgcctg gggccccccc cgactaacat ctctgcgagc
     7141 agattgaaaa tcgtttctgg tctgataggg agcgcatgct ccgagtagta aggccaaaat
     7201 ggccagggcg cgcattctca tagctgttcc tattatcggt ctaacttgat tagacgaatt
     7261 caggaaaaaa aaggtcagtc tctgaggacg cggccgcggt gggtgatgtc tttttctacg
     7321 gtgggcttct tttctctgga aaggagtggt ttgcgctctc ccggcgattt ctgctgattg
     7381 ccttccccgg aagcccgcga attcacgcgg gccattgcct gggtgctcat ctggtataga
     7441 atcgtaatga ctagagccag aaaaattagc ttcattcaca cctggagggc gcttttgcgc
     7501 cctatttgaa gttcttctga gacaaaacga tatccacctt tgtcacgtca ccatctttca
     7561 ccttgatgta ctggttgtcc agcttcaggt tttcctcaaa ggaagccttg atttcatagt
     7621 agttgcccgg aggcagattc atgaaccaga agctaccgtc cgtgagcgac ttcatcttgt
     7681 gcacctggtt ggtgcccacg actgtaggca tccagatagt ctggccagga agaggctgtt
     7741 cgaaattctt caggaagacc cggccctgga tagagccagt tccgcgcaga gaagctacca
     7801 cgaacttctt cactacattc ttaccttcta cgccaatggt ctctttgatc acaggataat
     7861 cttcggagat gatctctact tcatgaactc cgcctggcac accgttgact tcaatgagtg
     7921 ccactgtgtt tggaacaacg gtaggagttc cctggcgacc cactgtataa gttccaagag
     7981 gaatgtcttc tcctgtggcc gccttgagac cggcctggtc cacccaggtt acagttgcgg
     8041 gaagttcttt tccagagaag cgaactttca cagtgcgatc tgaatccaca ccgcgcatcc
     8101 ttctcatcat catggctgga tccggcgttt gcgttcctga aagctggctg cggagaatgg
     8161 ttgtggcgat gattactttg ccagtgtcgt ttgtattcgg attttccggt ggtggttcca
     8221 cgttaggcgg cgttggagtc acaggagtca caggagtcac aactggtggt tgcggtggct
     8281 ggggcggttg tggcttgttt ggatccggct gcggtgggtt cacaacagga gtcacaacgg
     8341 gtggctgcgg tgtgggaact tctctacccg cgaggaaaat tccctttgca tttccggaaa
     8401 tctgcggatt ctgatcgtga ttgaattgct tctttgccat gtctgcgact cggcgcgaag
     8461 accattcaaa tgcttcaagt gcggttacgg tgccgtcttt gttcaagtct ccgttccgcg
     8521 gatcgagacc ctgggcgaac cagtacgtga atactccatg attgatgctt ccgcgaactt
     8581 caatagacgt ttcgtttgaa tcactggaag cgatgatcgc tttgttctga aaatagaagt
     8641 tttcgtctcc gttttgaatc acggttccgt ccgatcctgg agaagtagga atatccccga
     8701 ctccgcgacg acctttcttc gcaatgccgc ctgagtagca gcagtccatg atcagagtta
     8761 cgtgctgtgt cttaatgcct ttgatccaat cgctcagctc cttatcagaa acgtgcgggc
     8821 gatcatacat tacaatgtag ttgcgaagcc cgttgggcgc actggcgtca cgctggtatg
     8881 tgccatgacc ggaaaagtaa atcgccacaa cgtcttcctt ccctgccttc tgcgcgattg
     8941 cttctagagt ggatttgacg cgcgaagatg tgaccattgg tccaagtaga accgttgcgt
     9001 catcatagga gccgttcgat ttcagagtct gctccatgag cttcgcatcc gactcgcata
     9061 gttcgagttc cggaataccg gcagagttgc cggtgtaatt tgagcccatc acaatggcga
     9121 atcgcttcgc ctcgacggca cccagggaaa gtgaaatgag taagagagaa aaaactgtct
     9181 ttttgaagtt catgttaccc ctgcataaat ggacgatgcc agtgtttcag ggttcttcct
     9241 tttttgcgag aaattcctgg atctggccgg aaacgcgggt ttcgaactcg tctcggctga
     9301 tctgggccag ggcccgttcc atgagcggaa ccatgccatc cagaacgtcc tggcaggccg
     9361 ccaataattc ggcctcctcc tgggttttag gagatgtagt gataggtttc tgagaacgga
     9421 gggattccga caggtccaga aatcgatcga cccgtccctc ctccagcatc cttcgctgcc
     9481 tgcgaaggcg gaagaggagg aagcgaagat cagccagtaa tggagaagct ggctccttca
     9541 cgtgcaggca ccgatactcg cggaggggac tgtctggaag cctggtccca ggcgtcgcgc
     9601 agctgctcga ggagtttgac cacctctgtg agcacttcgt tgtccttctg cacattgccc
     9661 tcaagaagtc tcttcttgat ataagcataa atattgaaga gattctgagc gatttccccg
     9721 ccctcttcca tattcaaaga aagcatcagt tcggttacaa tgtcctgcgc tcgaatgatg
     9781 ttgttgttca ccacatcgta ctttcgcgga gtcatgttag acatcgctac gttgatgaat
     9841 cggattgctc cttcatagag catgacgatg agtttcccct gccctgcagt ctggatctca
     9901 gtgtctttgt acgcttcgtg ggtctttttt gcagccatca taacctgaaa caagtatcgg
     9961 ttgaatcgaa aaaaccttga aaccgggaaa cgttattcca cgtagaattt gatcccctcc
    10021 gggtagcccg aaggcactag aatgcaggcg gatgttgcag atcagcacat cagggcatat
    10081 ccacgcatac gacggcaatg tcatcgcttt gcggactgga cccacgaaac tctagcaaat
    10141 catcaaaaag cgcggccaga aaagcttcgg aatccagaga actgtgtttg atgagcatag
    10201 attccagcct ttctccatac atggatcgat cctcagaaaa agtctccgtc aaaccgtcgg
    10261 tgtagaaaaa gaatcgatcc gatttcttag cctcaattgt gtacgatttc catttcaaag
    10321 atcgatccag acccagaagc agactatcgt ctccgagagt tgtgaggcgc ccgtcagaaa
    10381 gatgaaaccc gggcggatgg ccagcattcg catggatcac acgccgcgca gaaacatcaa
    10441 actgaacgca tacagcagtg acgaaatgtt gcccgcctgt tagaaattcg cacaaatctg
    10501 aattgatagc ggccaaaatc tcagacgggt caagcaaatg tttatgcgca tgaaaagaag
    10561 cttttgtcat tgtagtgatc aaggatgcgg ccacgccatg gcccgtcaca tccgcaacga
    10621 ggagcacagt cttgtctccc gctgtagcta catcataaaa atctccacct agattctgca
    10681 tggccagata acgtccgcgc aaggttaagc cgcttgaaag cgtcccaagc gtgggcaata
    10741 atccttgctg caaacgctct gccagctcga gttcgacctg aaagcggtcc gcggctgttt
    10801 tcaatttttg atgagccttc tccaggtctc gtttgttccg cctaacttcc gtcaggtgaa
    10861 cggaaagcgc caccctgctc ttcttcaatt gattggaagc gcgtgtcata tagaaagtca
    10921 tgatcacaac ccaggccatg gacaagatga ttcgaatctc aaaggcaccc agcacgaatc
    10981 cgggaatcgt ggccgcaaac ataatcagca cccacacaat ccagtatcgt ctgcgaatgt
    11041 aaccaaatcc tggcatacct acagcggttc cccagatcca gaaaacaatg gagtaaggag
    11101 gcagaacaaa agacaaatag ccgtaataaa tcgcgacagg aaaactcgtg atcagaacca
    11161 gatcaaacca ggactcaagg gagcgcgctc ctcgcacgag ccttctggac aaccacagag
    11221 caatgaagat gtaatacgaa acgaacgcaa tgaaaggaat ccactcaatg cgcggcccat
    11281 acacaggatc cacaaagcga atcaatagaa tggtggatga gtttacggcg agcccgtaaa
    11341 aatagacgga tgacgctggg ttaaacagcg atcgcatgcg actgaacatg cgaaactccg
    11401 ccatggcaaa ggatcgcacg tcccaatcag gttgtcaacc cacttttttc gagcttcttg
    11461 agctctcgcg atctgcgaat catgatttct cgcatgctga agttgaaaat cggggctgac
    11521 tctacaattt tgcgaatctc tccgcgcgcg atttccagaa caacactgtc tctgagtgca
    11581 gtcactgtag catttcgcac cgaatccccc agggacccat attccccgaa catttcacct
    11641 gcgcgaagaa tcttaacttc tgtaccgt
//
LOCUS       DAORFF010000029        11410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2533_length_11410_cov_3.736151, whole genome shotgun sequence.
ACCESSION   DAORFF010000029 DAORFF010000000
VERSION     DAORFF010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11410
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2533_length_11410_cov_3.736151"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..721
                     /locus_tag="PKK76_02260"
     CDS             212..721
                     /locus_tag="PKK76_02260"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNL67314.1"
                     /translation="MQQDPTVISQNPYEAPAVASLPVEEPYSSGTLADPGMRLIAHII
                     DMIVFLGAFGVAMAGSSFLPQEKMALGIFGGMFGALVIIAINLYLLAARGQSIGKVAL
                     GIRIVRVSGEKASFARIFFLRIGVPAIINVVPGLGSMFALVDSLMIFQDSRQCIHDMI
                     ADTKVVRIR"
     gene            725..1327
                     /locus_tag="PKK76_02265"
     CDS             725..1327
                     /locus_tag="PKK76_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67315.1"
                     /translation="MSSQDLAFARQYLEGRLREGQHRPDLHQTINSVYSRYLKILSDT
                     PDQYASSVGDMFDVWKSETIDRQLNLRKLAEKIGHAEAAAVHAKMAERVKATRSYPEL
                     ASLSAEVQEAARKAGASEIARRDALTAFLGFLVLIRAESEKNKPGRRAQARSAFQEMR
                     SLDPDFSFSKLRSHLPYRAMIYYTEEQLSMLEQTFAECKA"
     gene            1324..2355
                     /locus_tag="PKK76_02270"
     CDS             1324..2355
                     /locus_tag="PKK76_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67316.1"
                     /translation="MNADIEQYLKGQADMIATYVQKLGKDDPAVTACLAVLEKLRTLG
                     GRYAAIYEFMADPSFQPLSAEFSAKMMELATKASRPAPGAAPSIPSAAQAALGYHRAF
                     ESIANIAKLPRTREVYERIFALEKESAHAGEFVAKMAAEGLFAKMATVQMIEEYSGLT
                     AQAESVAQPTMAAHNEFVLAAAQKATSAIEIEYESMRLFELNQLELRLDRTLVNDAFD
                     ILGSAISSYLLVPSEENRAAVEAGYRFVADFYALDSETLFAIPRFMDHMRFMVEHSNK
                     SPGSKKMTVESMTSDFKGAITVCLKGKAPVVLGPSSRRTAVLWGKTVPLEKFHEAIRH
                     PDRPAELQT"
     gene            complement(2356..3261)
                     /locus_tag="PKK76_02275"
     CDS             complement(2356..3261)
                     /locus_tag="PKK76_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HNL67317.1"
                     /translation="MDIQFKNIPGLLADSIKKKPVWWILGTATAIVVVLALRPRPQPV
                     ETATVRRMTFEDTITEDGVTRVKEKFIILSPYSGNLVRLSRHAGDSVKKGEPVAFVFW
                     DRETAVPSPANGSILKVFREDAGPIEIGAPIVEVGDPSSLEIVLEMLTADAVKVAPGN
                     PIRIRGWGGADPLAAKVRIVEPQAIMKISGLGVEERRVRVLADIISPREKWKTLGDNF
                     RLECEVIHYRQDGALVIHSGALFRDQEGWSVFRVEKGKAHKTKVQVERRGSTQSMIKS
                     GLAEGDEVIVYPGEQIKEGVRISEL"
     gene            complement(3268..5616)
                     /locus_tag="PKK76_02280"
     CDS             complement(3268..5616)
                     /locus_tag="PKK76_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNL67318.1"
                     /translation="MSPLNLKAIREIKGLKSQTITTALVALCGIAVQIGYVSAYVSLN
                     SAKERFYTNSRFADIFATVKRAPESVLTTIRNIPGIQQAETGILYEASLRVAGLEEGA
                     SARIVSIGRHDVLNKIRVSEGRLPEVGSATEACVSEGFAHANHLGPGSTVEALINGKS
                     RQVHIVCIALSPEYIYAIRAGSFMPDDKHYGIFWMTREALTDIAGFGGSFNALAIALS
                     PGTKPPDVLKSVDRILDPYGNPGAYARDKQLSNVFLNNELYQLQVMGYSIPIIFLGVA
                     AFLLHIVIGRLINRQREQIATLKAVGYSNLQVGLHYLSIVTFMTLSGALPAILPGIWL
                     GQWTTEMYKDYMKLPDFHFVFDPMVPLWGILFAIVASFLGALSSLKAVMKLNPAEAMR
                     PPAPPRYEKTFIEKAARLTPFTLMGFRHVALKPFQSILSVAGLSMAGAIIVMGFIWTD
                     MVQFLLTVQFNSIQREDVMVNFQGPRPTNVLGEMRVIPGVIYVEGYRIAPIRLHAGHR
                     TKEMALTGLPEGARLRRILKKDFRPNPIPASGILLNESLAKTLSLHPGDIVTLELLEG
                     DRRQVSVPIAGLAEELLGSGAYMEISKMNALLREGPVISTAALSVDLSLLESVQRELR
                     SMPYILSVDSKLFVIRMFHSLYADMLLVMALMLIGFASVIAIGVVYNTVMVALAERSW
                     ELASLRVLGFTQGEVFQMLLVELTLQIIVAIPFGVLLGYHMNSAMLAGLPEDQYHFSI
                     ILQPYAVASCAGILIASAAVSSFLVWQRIKKLDLVSVLKVRE"
     gene            complement(5613..6323)
                     /locus_tag="PKK76_02285"
     CDS             complement(5613..6323)
                     /locus_tag="PKK76_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNL67319.1"
                     /translation="MSSQSVLTARNLMRIYEMGEVQVRALDGVDLDLFRGEFLVLLGP
                     SGSGKSTLLNLLGGLDVPTSGELTYREHDLKHAGEAELTRYRREHVGFVFQFYNLIPS
                     LTAKENVELVTQIAINPITAERALELVDLSARADHFPSQMSGGEQQRVAIARAIAKRP
                     DLLLCDEPTGALDIKTGQIVLEAIRRVNEEIGSSTVVITHNASIAGMADRVIQLADGK
                     VASETRNKKKLKPSEIHW"
     gene            6423..6842
                     /locus_tag="PKK76_02290"
     CDS             6423..6842
                     /locus_tag="PKK76_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67320.1"
                     /translation="MVSVSIGRMRLWSKISGELLVAALFFVLLSPLLGVHVHDNGIVH
                     SHSRSTSHNHSDKTIPQNEIPSLPLKLVFNKSLPLEVAHSLLFFVLFLAMFSSHAIAQ
                     PAVVRSVSVRSIRDGTLFLPSPPRRIAGQRAPPISLT"
     gene            6890..7222
                     /locus_tag="PKK76_02295"
     CDS             6890..7222
                     /locus_tag="PKK76_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67321.1"
                     /translation="MLKSYIVLIALSFAAVGSLAAEGRGWLRGSEVLDSRSDVHIANV
                     SQIQPRVGDEFDVFRIILDANPALRGMRHRYSKLGRVRVVEIVDPHTVRASVIWGLTG
                     DSYLIQTE"
     gene            7396..7665
                     /locus_tag="PKK76_02300"
                     /pseudo
     CDS             7396..7665
                     /locus_tag="PKK76_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596316.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            8639..9691
                     /locus_tag="PKK76_02305"
     CDS             8639..9691
                     /locus_tag="PKK76_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010679700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNL67322.1"
                     /translation="MKAAVLYKDQTHLRIEDVPIPELKPGDVRVNIKCCGVCGSDIHM
                     TIHKQVRLRHYPTILGHEASGVVTEVGSDVKKFKKGDRVVVCSGLGCGKCRQCLKGRH
                     NLCPEINVLGAEMNGAYAEQITIEERHLYHLPDQIPFDQGAILADAVSTPYHALKFVG
                     KIEPGEVVAVYGCGGLGIHAVTLARALGASKVIALDVDEGALDNAGKYKADEMIDLRR
                     VRNSGKTLQELTGGVDLMLDFSGHYSNFEDAMRAMNPGGRMVMAGIGKHPFKLTFPFM
                     VINKQISVLGSLGCDSRAIPELIDLYTSGRLDLSTSITSHHPLEDVNQCLENLYYRKG
                     NPIRFIITPNGKIGAQ"
     gene            complement(9685..10263)
                     /locus_tag="PKK76_02310"
     CDS             complement(9685..10263)
                     /locus_tag="PKK76_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNL67323.1"
                     /translation="MRKFILILCVLSLFAQCKKEIHPITSIPDDSLYQLDSEWKTQKN
                     TVIHLRDLSGRVLIVGLIFTRCTAICPTIVADMKTIEKNIGWFHKDDVGFLLVSIDPT
                     RDTPEAMRSYMKKMNLDESRWTFLQAKPPDVADLASLLGVKISPLPDGSFSHSRILTV
                     LSPSGQIKFQDPRVSEDGANFAEAARQAADFH"
     gene            complement(10260..11009)
                     /locus_tag="PKK76_02315"
     CDS             complement(10260..11009)
                     /locus_tag="PKK76_02315"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNL67324.1"
                     /translation="MSAARLTVLSLALLAFELSAATPDGMSFVPPGVFQPFFKNIAPQ
                     KVRGFYLDQTQVTNAEYLIFLKANPSWEKGSAKRIFADAGYLRGWTSPGSFPAESAEA
                     PVTDISQFAARAYCTWRGKRLPTLAEWEYAAGADPSHPMTDAENARLLAWYERNSTGR
                     PGRVRNVSNLHGIYDMHGLVWEWVDDFNSVMISDESREGGSDKQMFCGGGAANAADFR
                     NYAAFMRYAFRSSLRSNFTSSSLGFRCARNQ"
     gene            complement(11009..>11410)
                     /locus_tag="PKK76_02320"
     CDS             complement(11009..>11410)
                     /locus_tag="PKK76_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNL67325.1"
                     /translation="TVYLPEGGAIQSIKEAQEAQKPLSRDESIAAGKIVYTQVCMACH
                     QANGEGMPNAFPPLAKSDFLMKDKQRAIRIVMNGLQGEVVVNGNKYNSVMPALGLKDA
                     DIANALNYVRNTWGNSAPDTITPAEVKALRK"
BASE COUNT         2723 a         2856 c         2999 g         2832 t
ORIGIN      
        1 gtgcgaagaa taggaggaaa tcaagtctgc ggtttttaga gaagatgacc agattgtgaa
       61 tggcgcgata aaccaggatg gccgaaacga gaatgtaggt aagctcgtag acggtcacca
      121 tgcaaataga cggaggaaag cagtatattt caatcaataa tcatggctgc tcattccttg
      181 acagcaaaga ttggccattc gaacttcacc catgcaacag gaccctaccg tcatcagtca
      241 aaatccatat gaggcacctg cagtagcgtc gcttcccgtg gaggagccat attcctccgg
      301 gacacttgca gatcctggaa tgcgcctcat tgcgcacatc atcgacatga ttgtgtttct
      361 cggggctttc ggtgtggcca tggcagggtc ctcttttctt ccacaagaga aaatggcgct
      421 tggaatcttt ggcggtatgt tcggggctct cgtcatcatc gcgatcaatt tgtacttact
      481 ggcggcgcgg ggacagtcca tcggcaaggt tgcacttggg attcgcattg ttcgagtgag
      541 cggggaaaag gccagcttcg cgcggatctt ttttctccgc ataggcgtcc ccgcgattat
      601 taacgtggtt cctggactcg gttctatgtt tgcgctcgtg gattctttga tgatttttca
      661 ggattccagg cagtgcattc atgatatgat tgcagatacg aaggttgttc gaatcaggtg
      721 attcatgagc tctcaggatc tcgcgttcgc acgtcagtat ctcgaaggcc gtttgcgcga
      781 aggccagcat agaccggatc ttcatcaaac gatcaactct gtttacagta gatacttaaa
      841 aatattaagt gatacaccgg accagtacgc ctcttcagtc ggagatatgt tcgacgtgtg
      901 gaaatcggag accatcgata gacagctcaa tcttcgcaaa ctggcagaaa agatagggca
      961 cgcagaggct gcagcggtcc acgcgaagat ggcagaacgt gtgaaagcaa ctcgttccta
     1021 tccggagctg gcatccctgt ctgcagaagt gcaggaagcc gcgcgcaagg ccggcgcatc
     1081 tgaaatcgcg cggagggacg cactgaccgc attcctgggc tttcttgttt tgattcgcgc
     1141 ggaatccgag aaaaataaac ctggccgccg ggcacaggca agatctgcgt ttcaggaaat
     1201 gagatcgctc gatccggatt tcagtttttc aaaattgcga tctcacttac catatcgtgc
     1261 catgatctac tacacagaag aacaactgtc catgctggag caaacttttg cggagtgcaa
     1321 ggcgtgaatg cagatataga gcaatacctg aagggtcagg cggacatgat cgccacctac
     1381 gtccagaagc tgggcaaaga tgacccggct gtgaccgcat gcctggcagt gctagaaaaa
     1441 ctaaggaccc tgggtggacg ttatgcggcc atctacgagt ttatggcaga tccttccttt
     1501 cagccgcttt ccgctgaatt ctcagcgaag atgatggagc tggctacaaa agcttctcgt
     1561 cctgcgccgg gggccgctcc gtcgattccg tctgccgcgc aggcagcact tggataccac
     1621 cgcgcttttg aaagcattgc gaacattgca aagttgcccc ggacgcgcga agtgtatgaa
     1681 cgtatctttg cgcttgaaaa agagtctgca catgcgggtg aattcgtcgc gaagatggcg
     1741 gcggaaggct tgtttgcgaa gatggctaca gtacagatga tagaagaata ctccggtctc
     1801 acggctcagg ctgagtctgt ggctcagcct actatggccg cacacaatga gtttgtcctg
     1861 gcggccgcac aaaaggccac gagtgccatt gaaatcgaat atgaatccat gcgcctgttt
     1921 gaactcaatc agctggagct tcgtctggat agaactcttg taaatgatgc atttgacatc
     1981 ctcggatccg ccatttccag ctacctgctg gtcccgtccg aagaaaatcg cgcggccgtg
     2041 gaagcgggat atcgctttgt cgcggatttt tacgcactgg attctgaaac gttattcgcc
     2101 attcccaggt tcatggacca tatgcggttt atggtggagc actccaataa atcgccgggt
     2161 agtaagaaga tgacggtaga gtccatgact tctgacttca agggggccat cacagtctgc
     2221 ttgaagggaa aagcacccgt cgttctgggt ccttcgtctc gcagaacagc agttttgtgg
     2281 ggaaagaccg taccattaga aaagtttcac gaggcaattc gtcatccgga tcgcccggct
     2341 gaattgcaga cctgattaca gttcggagat tctcacgcct tctttgattt gctctccggg
     2401 gtaaacaatg acttcatctc cctctgccag accggacttg atcatggact gggtgctccc
     2461 gcgacgttcc acctgcacct ttgttttgtg cgcctttcct ttttctacgc gaaatacaga
     2521 ccatccttcc tgatctctaa acaacgcgcc gctgtgaatg acgagcgctc catcctgtct
     2581 gtagtggatg acttcgcatt ccagtctgaa attgtctcca agcgttttcc atttttcgcg
     2641 aggtgagatg atgtctgcaa gtacgcgcac gcgtctttct tccactccca ggccggatat
     2701 cttcatgatt gcttgcggtt ctacaattct cactttcgct gccaggggat ccgcaccacc
     2761 ccatcctcga atgcgaattg gatttccggg agccactttc accgcgtctg cagtcagcat
     2821 ttccagaacg atttcaagtg aagatggatc acccacctct acgatgggag ctccgatttc
     2881 gattgggcct gcgtcctcgc gaaatacttt caatatggag ccgtttgcag gcgatggaac
     2941 ggccgtttcg cgatcccaga atacgaacgc tacaggctcc ccttttttca ctgaatctcc
     3001 tgcgtgtcgc gaaaggcgca caaggtttcc gctataaggc gaaaggatga tgaacttttc
     3061 ttttacacgc gtaacgccat cttctgtgat tgtgtcttcg aacgtcatac gacgaacagt
     3121 tgctgtttcg acaggttgtg gtcggggtcg cagtgcgagc accacgacga ttgcagtagc
     3181 tgtcccgaga atccaccaca ctggcttctt cttgatggaa tcggccagca ggcccgggat
     3241 gtttttgaat tgaatgtcca tgatcactta ctcgcgcact tttagaacgg aaaccaggtc
     3301 cagctttttg attctttgcc agacgagaaa agaggatact gccgcagatg caatcagaat
     3361 accggcgcag cttgcaacag catacggttg aagaatgatc gagaagtgat attgatcttc
     3421 tggaaggcct gctagcatcg cggagttcat gtggtaaccc aacagcactc cgaatggaat
     3481 ggcaacgatg atctgaagag tcagctctac gaggagcatc tggaatactt ctccttgcgt
     3541 aaatccgagt acgcgaaggc tggccagttc ccaggatctc tccgcgagag ccaccatcac
     3601 agtattgtag actactccga tcgcaatgac actcgcaaaa ccaatcagca tcaaagccat
     3661 gaccagaagc atgtcagcat agagactgtg gaacatacgt attacgaata gtttagaatc
     3721 cacagaaaga atgtatggca tggatctgag ctccctctgt actgattcga gcaaactcag
     3781 atccacagaa agagctgcag tagaaatcac agggccctcg cggagaagtg cgttcatttt
     3841 ggagatctcc atataggcac ctgatccgag cagctcttcc gccaacccgg cgattggaac
     3901 ggatacctgt cggcgatcgc cttccaggag ttccagcgtt acaatgtctc cggggtgtaa
     3961 agaaagcgtt tttgccagag attcgttgag taatatgcct gaggcgggaa tcggattggg
     4021 tcgaaagtct ttctttagga ttctgcgaag cctggcaccc tcaggaagac ctgtgagtgc
     4081 catttctttg gttcgatgtc ctgcgtgcag acgaatgggc gcaattctgt aaccctctac
     4141 atagataaca cccggtatga cgcgcatttc tccaagaacg ttagtcggcc ttggtccctg
     4201 gaagttcacc atgacatcct cgcgctggat gctgttgaat tgcaccgtga gtagaaattg
     4261 gaccatgtcc gtccagatga aacccatgac tatgatggca ccggccatgc tcaatccggc
     4321 gacagaaaga atgctctgaa agggcttgag ggccacgtgt ctgaatccca tgagggtaaa
     4381 gggagtcagc cttgccgcct tttcgatgaa agtcttttca tagcgtggtg gagctggagg
     4441 tcgcattgct tctgccgggt tgagtttcat cacagccttc aggctggaga gcgcacccag
     4501 gaaggaagca acaatcgcga aaagaattcc ccacagtgga accattggat caaatacaaa
     4561 atgaaaatcc gggagcttca tgtagtcttt gtacatttcc gtggtccatt gccccagcca
     4621 gatacccggg agaatggcag gaagagcgcc gcttaacgtc ataaatgtga ctatggacaa
     4681 atagtgtaga cctacctgaa gatttgaata tcccactgct tttagagtcg caatttgttc
     4741 cctttgtcgg ttgatcaatc ttccaatcac aatgtggagt aagaatgccg ccactcccag
     4801 aaagatgatt ggaatgctgt agcccataac ctgcagctgg tagagttcgt tgtttagaaa
     4861 aacgttagat agttgtttgt ctcgggcata cgctcccgga tttccatagg gatctagaat
     4921 tctatctaca gattttagaa cgtcaggcgg ctttgttcca ggactcagag ctatggccag
     4981 tgcgttgaat gaaccgccga accctgcaat gtccgtgagt gcttcgcgcg tcatccagaa
     5041 gatgccgtag tgcttgtcat cgggcatgaa gctgccagct cgaatcgcgt aaatatattc
     5101 cggtgacaga gcaatgcaca caatgtggac ctgcctcgat ttgccgttga tgagggcctc
     5161 tacagttgag ccagggccca gatgatttgc atgtgcgaaa ccctcactca cgcaagcctc
     5221 tgtggcactt cccacttccg gcagtcttcc ttcgctcaca cggatcttat tcagaacatc
     5281 gtgacgacca atcgaaacaa ttcgcgccga cgcaccttcc tccagaccag caactcgcag
     5341 gctcgcttcg tatagaattc cagtttccgc ctgttggatg cccggaatat ttcggatggt
     5401 agtgagtaca gactccggag ctctttttac ggtcgcaaaa atgtccgcga aacgagaatt
     5461 tgtgtagaat cgctcttttg cgctgttaag agaaacgtac gcgcttacgt agccaatctg
     5521 gactgcaatg ccgcataacg ctaccagggc cgttgtgatc gtctggcttt tgaggccttt
     5581 gatttccctg atcgctttga gattgagagg gctcaccagt gaatctcgga tggtttaagt
     5641 tttttcttgt ttcgagtttc ggaggcaact tttccgtcgg ccagctgaat taccctgtct
     5701 gccattcctg caatggaggc gttgtgagtg atgaccacag tggaggagcc aatctcttcg
     5761 tttacgcgac gaatggcttc tagaacgatt tgccctgtct taatatcgag cgcgcctgta
     5821 ggttcatcac acagaagcaa gtctggtctt tttgcaatgg ctcgcgcgat agccaccctt
     5881 tgttgttccc cgcctgacat ttgagaaggg aagtggtctg cgcgggcgga aagatccacc
     5941 agttcgagcg cgcgttctgc agtgattgga ttgatcgcaa tttgagttac aagttccacg
     6001 ttttcctttg cggtaaggct tgggatcagg ttgtagaact gaaaaacgaa accaacgtgt
     6061 tcccgtctgt atcgcgtgag ctctgcttct cccgcatgct ttagatcgtg ttcgcgatac
     6121 gtgagttctc cagacgtggg aacatccagt ccgccgagca aattgagtaa cgttgatttg
     6181 ccgcttccag acgggcccag aagtaccagg aattctccgc gaaacaaatc cagatccaca
     6241 ccgtcgagtg cgcgtacctg cacttctccc atctcataga ttcgcatgag attgcgcgct
     6301 gtgaggaccg actggctgct catgccccat ctcgcatccg cgcgtgctcg tttcaagtga
     6361 tttgctgcgg atgcaggacg gtttcgcgtt gcgcgaaata gattttaatg gccagaattt
     6421 gtatggtatc cgtaagtata ggtaggatga gactctggag caaaatatct ggcgaactgc
     6481 tggtcgcagc attgttcttt gttttgctga gccccctgct gggcgtgcat gttcacgaca
     6541 acgggatcgt gcatagccat agccggtcta ccagtcacaa tcattctgac aaaacgattc
     6601 cgcagaatga aatcccatcg ctacctttga agcttgtttt caacaaaagc ctgcctctgg
     6661 aagtcgcgca ttctttgctg ttctttgttc ttttcctggc catgttttct tcgcatgcca
     6721 tcgcgcaacc ggccgttgta agatcggttt ctgtcaggtc cattcgtgat ggaacattgt
     6781 ttttaccatc acctccgcgc agaatcgcag gccagcgcgc acctcccatc tccctcacct
     6841 gatctggttt tcggcccatt ctgttggccg aatgaaaatg gagggaatta tgttaaaaag
     6901 ttatatagta ttgattgcac tatcgtttgc cgcagtgggc tcactggcgg cggaaggcag
     6961 aggatggctt cgaggaagcg aagtcctgga ttcgcgatcg gatgttcaca tcgcaaatgt
     7021 ttcacaaatt caaccgcgcg taggagatga atttgatgtg tttcggatta ttctggatgc
     7081 gaatcccgct ctcaggggta tgcgccaccg ttatagcaaa ctcggacgag tgcgcgtagt
     7141 ggaaattgtg gatccgcaca cggtcagagc cagcgtgatc tggggactaa ccggcgattc
     7201 gtacttgatt cagacggaat gaagcgaagc cagatggaca atctgtccat ctggcattca
     7261 ttgaagaaaa agcctccata gaggttgcca gctgggaagg aatgtgattc gattgaatct
     7321 catttgccgg agtgaggttt gggattggag tgagacatga cggaacataa atcgcatcac
     7381 acagaacaca ctcgagccat cgaaagcgca ggcgtgactc ttgtgaaagg tgacctcatg
     7441 ggaattgtcc gcgcgatcaa actgagcaga gcaacaatga ggaatatcag gcagaatttg
     7501 tttttcgctt ttgtttacaa tgcgctcgga atcccggttg ccgcagggct gctctatcta
     7561 ttcggagggc ctcttctcga tccaatgatc gcgagtctcg caatgagctt gagttcagtg
     7621 tcggtgatcg tgaatgcgct tcgtttgcgg ttgctcaagt tgtagtcgct gtttgcgagc
     7681 atggtggaat ctgacctgac cccgtggatt caggaccgtt agaatggaga gtgtgaatag
     7741 ctgccgtctg gcagagggga attaggaaga ttgacagctt gtgaaaccgt gctcattgtt
     7801 gcacacatat taaattctcg ggcgcggtgc aaactgcgct cgtcgcgaag atagccgcgg
     7861 agttcacctg agtttggtct cctaattctc caaggccagc cgcactcgac catcccgtac
     7921 acgtatttgc ggccgttcgc caatcggctg tgctggcaaa accagccgca taatacaacc
     7981 ccgacgaatc cagagagttt gtgagcggga agacgaaaat tcccgccgac gccatggtcg
     8041 tcgcaatcgt ttgccggctt accaagcgcc tgtaagttgc cctggggcgc aagacccaat
     8101 tcatgtgctc cgccgaccct ccggcgcaat ttgcggtgct gcacgctcgg cgagttgtac
     8161 cgtccacgat caacgcttta taaataccgc tccccggata ggcagaatcg ctcatgcacc
     8221 tcgcatctgc ccccgcaacg ccaccaagga ctagaggagt gtagccaaga ctcgtagtgt
     8281 agacaaagta ggatgcagga gaactcaggt tcgccaccag gagctcactc actgtcgtcg
     8341 gatccaaata caatccgccg ccaggtgaat ttgtgcaacc agtgacgcaa aatgacacaa
     8401 cagcagcaat tgcgatcagt aattttgtca atcgccgtac cccgcgatta ttaggctcta
     8461 agaatcggca aggaggtcaa tctcaattca gagcaccttg atcgcataac ggatcgcagc
     8521 cagagccggg cccgaaaaaa caatcgacac tccgcgaggt tcgcgtcagt ctcgggccgg
     8581 tccctaccat tcgcggatca ttgcgcaccg tcccgatacg acctttgcgg agggaaaaat
     8641 gaaggcagct gttttgtaca aagatcaaac tcatctcagg attgaggatg ttcccattcc
     8701 ggaactcaaa cccggtgatg tgcgtgtgaa cattaaatgc tgcggcgttt gtgggtctga
     8761 catacatatg accattcaca aacaggttcg ccttcgccat tatccgacga tcctgggcca
     8821 cgaagcgtca ggtgtggtta cggaagtcgg atcagacgta aagaaattca agaaggggga
     8881 ccgcgttgta gtgtgttctg gtcttggatg cggcaaatgt cggcagtgtt tgaaaggcag
     8941 acacaacctt tgccctgaaa tcaacgtgct gggcgcggag atgaacggtg cgtatgcgga
     9001 gcagatcacc attgaggaac ggcacctcta tcatcttccg gatcagattc cttttgatca
     9061 gggtgcgatt ctcgccgacg ccgtgtcgac gccatatcac gcactcaaat tcgtgggcaa
     9121 gatcgaacca ggagaagtgg ttgcagtcta cggatgcgga ggactcggaa tccacgcggt
     9181 cacgttggcc cgtgcgctcg gtgcttcaaa agtcatcgca cttgatgtag atgagggcgc
     9241 gcttgacaac gcgggaaaat acaaagcgga cgagatgatt gatcttcgtc gcgtgcgcaa
     9301 ttctggaaag actctccagg aattgactgg aggcgtggat ctgatgctcg atttttccgg
     9361 tcattactca aatttcgaag acgccatgcg cgctatgaac ccgggcgggc ggatggtgat
     9421 ggcgggaatc ggcaagcacc cgttcaagtt gacgtttccg tttatggtga tcaacaagca
     9481 gatatcggtt ctgggatccc tgggatgcga ttcgcgcgct atcccggagt tgattgatct
     9541 ttatacgagc ggccgcctgg acctgtcgac atccatcaca tcgcaccatc cactcgaaga
     9601 tgtgaatcaa tgcttggaaa atctatacta tcgcaaggga aatcccatcc ggtttatcat
     9661 caccccgaac gggaagattg gggctcaatg aaaatcggcg gcttgcctgg ccgcttccgc
     9721 gaaattggcg ccatcttcgc ttacgcgagg atcctggaac ttgatctgtc cggatggact
     9781 gagtacagta agaatgcgcg agtgagagaa gcttccatcc ggaagcgggg aaattttcac
     9841 acccagcagg gaagccaggt cggcaacgtc aggcggcttt gcctggagaa aggtccaccg
     9901 tgattcatct agattcattt tcttcatgta actgcgcatc gcttctggag tatcacgggt
     9961 tggatcaatg gaaacgagca gaaatcccac gtcatctttg tgaaaccatc caatgttctt
    10021 ttcgattgtt ttcatgtccg cgacgatggt cgggcaaatg gcagtacacc gtgtgaagat
    10081 caatccgaca atcaagactc ttccggaaag atcgcgaagg tgaatgactg tgttcttttg
    10141 tgttttccat tctgaatcaa gctgatacag agaatcatcc gggatagatg tgatcggatg
    10201 tatttccttt ttgcattgag caaagagcga aagaacgcaa agaattagaa tgaatttcct
    10261 cactggttcc tcgcacagcg aaagcccaga gaggaagaag taaaattgga acgtaagctg
    10321 gatctgaaag catatcgcat gaaagcggcg tagttgcgaa aatcagccgc gttggccgct
    10381 ccgcctccgc aaaacatctg tttgtcggaa ccgccttcgc gtgactcatc ggagatcatg
    10441 actgaattga aatcgtccac ccattcccag acgagtccat gcatgtcata gatgccgtgc
    10501 aaattggaaa cattccgtac tcggccaggt ctgcccgttg aatttcgttc gtaccaggca
    10561 agcagtctcg cgttctctgc atccgtcatt gggtgtgaag gatccgctcc agcggcgtat
    10621 tcccattcag caagagtagg gagacgtttt ccccgccagg tacagtaagc ccgtgctgcg
    10681 aattgcgaaa tgtccgtgac cggggcttct gctgattcag caggaaaaga tccgggcgat
    10741 gtccatccgc gaaggtaacc tgcatctgcg aagattcgtt ttgcggatcc tttttcccac
    10801 gatggatttg ctttcaagaa gatcaggtat tctgcgtttg taacctgagt ctggtcgagg
    10861 tagaagccgc gcactttttg cggtgcaatg tttttgaaga aaggctgaaa aacgccaggc
    10921 ggaacaaaag acattccgtc tggcgtcgca gctgacagtt caaaggcgag caaggccagt
    10981 gaaagaacag tcagccgcgc agcactcatt atttccgcaa cgccttcact tctgcaggcg
    11041 taatcgtatc aggggcagaa ttgccccagg tgtttcgaac gtagttgagc gcattggcaa
    11101 tgtctgcatc tttcagtccg agtgcaggca ttacactgtt gtatttgttt ccatttacaa
    11161 ccacttcacc ctgcagacca ttcatgacga tgcgaatggc gcgttgcttg tccttcatga
    11221 gaaagtctga ttttgccaga ggtggaaatg catttggcat gccttctcca tttgcctggt
    11281 ggcaggccat gcatacttgc gtgtatacga tttttcccgc agcgatgctt tcatctctcg
    11341 aaagaggttt ttgcgcttct tgggcttctt tgatggattg gatggctcca ccttcgggga
    11401 ggtagacggt
//
LOCUS       DAORFF010000030        11327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2565_length_11327_cov_4.273598, whole genome shotgun sequence.
ACCESSION   DAORFF010000030 DAORFF010000000
VERSION     DAORFF010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11327
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2565_length_11327_cov_4.273598"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2181)
                     /locus_tag="PKK76_02325"
     CDS             complement(<1..2181)
                     /locus_tag="PKK76_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNL67326.1"
                     /translation="MYAHSILAAALKPDAFRFLFHPEPVVDFPEGAPVERVEMEKLAI
                     HKDAQGVILAKKRDTKLFVDPVTGSFHITLPDETRLSGTVQTGKFGPPSFVVRLMPGD
                     SVFGFGCATGGLVKAGSFELKTIDTIFYSIDQASYSAFPFFVVRRGSSFIGIFLNCIR
                     PTRVEVDLEGKTRPEGPEVRFDIQSTEAVPIDVFVMSGNLRQVLAAYARITGRPFLPP
                     LWALGYHQSRWSYRTQKRVMELAERFRALDVPCDAIHLDIHYMDKYKVFTWDKSTFPD
                     PKGMNESLDKLGVKTVAIVDPGVALEEEYDTYESGRNEDAFCRDSMGNYFKGKVWPGT
                     TVFPDFTLENVRKWWAHQHAVLFRNGVSGIWNDMNEPTWKMGKKTDPLLEDVHFKNHS
                     QAEMRNLYANLEAEATNLAFEKFKKGERPFVLSRSGFSGIQKYACMWTGDNHSSWQQM
                     KENLHMVLNLGLSGVPFSGADAGGFGSGKGMLAIIKWRRDPELFARWMELASLMPFFR
                     AHTVLFGRDQEPWSFGESVLAICRKHIRRRYRLLPYIYNLFHEASEDGSPIVRPLFYE
                     FPNVKDEDCEDQFLLGSSILAAPVFERKAANRRVNLPDAGEGWFDFDTGVVRDAGRHT
                     YSTRPGMYPLLVRAGTALPMAVVGRNALDTMKSPLAFEIFPGTQISGKCILDDGISAD
                     GRNARIEVSGKKDRSGSIQLEVQVRENGFSPFQKTIRLRLPGNYR"
     gene            2270..3265
                     /locus_tag="PKK76_02330"
     CDS             2270..3265
                     /locus_tag="PKK76_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNL67327.1"
                     /translation="MRRKIALFSTLGVFVILGAVIIPRPGYHYQPHSLPADFDSFYAM
                     KLKQSKEKGARPGCEEKLNRFGPKTEYAILYLHGYGASRAEGEESIDRFANKLHANTY
                     YVRLPGHGTNMEDQASVSYTDYLDESEEALQMMPALGDKIIIVGTSMGALLATHLAAE
                     HPDKLYALIIASPFYEFVRKEANIASVPGGGKLAEVFGGGKIRDVTKKPDDVKDKRSP
                     GFENYWYEKQYFSSIQSLARLKKAVSGEHTFGRVTAPVLMIYYYKNENEQDTAASVPA
                     MLEGFHQFGKFTRPSPYNRSVAVADGDHIMFSKWIPTDKDLIDKELNRFIDDLKK"
     gene            3262..3987
                     /locus_tag="PKK76_02335"
     CDS             3262..3987
                     /locus_tag="PKK76_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNL67328.1"
                     /translation="MKTLIIIPAYNEESTIYEVVTKTLKHADVSVTNDGSRDRTPEIL
                     RTIQAECSSGKHAHKLNVVTHEKSTHIPKGLQDGFRYGVENGYDQFITMDAGMSHDPD
                     AIPDFLAADSSLDVVIGARKQVENVPFYRRVISRCAALVVNYALTPSYFRLGPGLSDC
                     TSGFRRYSHRAGLLISATELKSKAFDFHMEALALCVRAGMKPGEIAIRYVFSNSSFNS
                     RVLKQAMKFGMYLISTKGKAAAS"
     gene            3984..5258
                     /locus_tag="PKK76_02340"
     CDS             3984..5258
                     /locus_tag="PKK76_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67329.1"
                     /translation="MKSVNQAGLLTALRSVRAAPLLFALYAGTACLAIVARYDFDSTA
                     LIRFGHRYIAQNQDLTPTSAIRFRGNEANGGNGYDGQIFYYYASTMWKKGQWPVGFSN
                     AYRAPRAGYPFLASLFSWMGNRGTAYGMILVQVLSIVGGLICFWHLLPDERRSWTLLL
                     AVSPFVLQSFAFLVSDAVMISLAIIGIYFSRKNSVAASLLAWIFFSLAVMTKESSLFI
                     LFPYGLWALLRKDWKLCVVVAGSLVPMVCWQLYLFRAHGMVPASILSTFLSPLLGIRG
                     VASQTADLLRMKPFPIVEIFKVSAKWLLILLLPVSAWTALRGFKLADLQKISTPFRLA
                     ALFVVLSICIADFQYFWGIFENVGRMFTALVPAVILARSETKTDTSWKAFVLLLCALS
                     FLVWARICFLSPVFPFDHYIPYSGPVYPAPPL"
     gene            5328..6191
                     /locus_tag="PKK76_02345"
     CDS             5328..6191
                     /locus_tag="PKK76_02345"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNL67330.1"
                     /translation="MSISQVDLRQTRLPIQPQIISALMQLTDDSEVGFRDLEKIIKAD
                     ENISTLILKAANSPLYSRGKEIRSIQIAISLLGFRVIRTLATVASSQGMFQSGNYARF
                     RKYVSEHAVITGVIARSIAVKLKLKNLEEDAFVGGLLHDLGKVIMNSLDRSHYIEVIH
                     LVERGVPFSQAEKQIFGFSHRDIGEKAAMTWNLPQIYRWILSTHGLDALTPPEGMDES
                     SRALVQIVACANYLARLYGYGAPLSGIEHEISKFFDALGMDSDTRSFYLSEFRENIPK
                     DEFYNFFITIL"
     gene            6197..7894
                     /locus_tag="PKK76_02350"
     CDS             6197..7894
                     /locus_tag="PKK76_02350"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL67331.1"
                     /translation="MLQQSTLEFEIRLYDLFSEEISALRDAVNRLADAVDPVSLRGPL
                     NAIVYELCGNALKALYKRAYSKYVVAKLGLDDLPYHDWLKLFRAEIEAHRAENFAHLC
                     RQENIYIKASGKFAGDVFRIQIENDGAPSEIEMSRIHLALERAKETMSLAAYFDDEDA
                     YQEGAGLGLPMVMVSLRGMGVDPANFQIFTQGDTTVARIDVPLNLFGNARSEPLRLLR
                     QNKELLAITWNLFQQIDLSAVRFDLDGHILAVSRSMLKRLAIPLETPDMFKTLVPQRF
                     VAELFRGPQGIRVTQKFLNHRVYLENLSRTERILFNVSGYVTDKGVVSTLWQEIAISD
                     SAAPKLNEGSLIGNLKFHNIIEPYIPQLVLMKARETVELGRTELPEEVRDQTIFFADL
                     AGFTEMSESMRPHVVVEMLNMALSIMVRHIKEAGGSVDKFMGDAVMAVFADPLPACKA
                     ALEIQNTFAEVNRFRIEAKEPPVRARIGIHCGNVIMANIGTDERKDWTAIGDTVNTAS
                     RIEKNAPVGSILISDRVFERVKDDLRVTQSYRIKVKGKAKEISVYELEAMNSAPADS"
     gene            complement(7899..8777)
                     /locus_tag="PKK76_02355"
     CDS             complement(7899..8777)
                     /locus_tag="PKK76_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNL67332.1"
                     /translation="MKRLLAVILPVALLAAGAVYFSSAILYTGKYACSEEHFVYCGDP
                     SSLGLGFENIEFRTSDDLMIRGWWIPGRAGAPAIVVVHGRGVDRHEGLRFAPALHSAG
                     FNVLYIDLRNCGQSQKAFSSMGYYERRDVHAAVDYAVSRGMHSIGVFGFSMGAATSIM
                     AMSEDLRIRAGIFEGGFASFSSAISDAAWQDYKIPRYPLLPLVGALVSLRARMNTEEI
                     VPVKMISEISPRPVFIIHGTADVRVLPHNGEELYAAAREPKWYWVVPGGRHTRAWQAD
                     RAQAEAKVTEFFLKYL"
     gene            complement(8774..9382)
                     /locus_tag="PKK76_02360"
     CDS             complement(8774..9382)
                     /locus_tag="PKK76_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNL67333.1"
                     /translation="MSVDAVIFDLGKVLLDFDLEKCFSVLSRSTGVSADRVRSAMFSE
                     KMVEFERGVLSPEQFFEHVSIQIDGAADGPTIREAFDDIFTAMNENIRLAEMLSQRVP
                     VGLVSNTNQSHSSFVEKNYKFFHIFKAKIYSHEHALRKPEPDIYHRAIDLLGVPAERT
                     LFIDDLEKNIEGARAIGMAVVHLPPGKPLLPELQAAGVQGIA"
     gene            complement(9400..9720)
                     /locus_tag="PKK76_02365"
     CDS             complement(9400..9720)
                     /locus_tag="PKK76_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67334.1"
                     /translation="MRTFLFIAIFAGFTTALLAREIQFDVTNETAQKITTLTALEKGA
                     NRRIPLFVGKGIKPGITATVVWATFAEEGDCLWTVQAKFENGTLSKPVAVDFCKQKEF
                     SIKE"
     gene            complement(9792..11021)
                     /locus_tag="PKK76_02370"
     CDS             complement(9792..11021)
                     /locus_tag="PKK76_02370"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017853437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNL67335.1"
                     /translation="MALDYKSIRQDFPILQTMMNGKPLVFLDSAASSQKPQRVIDAFE
                     DYYRCRNANIHRGAYRLSYEATDLYDGTRKKMAAFLGAESPDEIIFTRNTTESINLVA
                     YSWGLSNLKPGDEVLVSELEHHSNLVPWMIAAKKTGAVLKHLPLTEDHKYDYSKIDSV
                     LSPKTRMVALAHMSNAVGTIHDIELLAKKAHAVGALFSVDGAQGACHLPVDVRKIDCD
                     FYSLSAHKMLGPTGVGVLYGKRKLLEGMDPFLGGGDMILTVQKDSYLPAELPMKFEAG
                     TPNIAGVVAFAIALEYLRQAGLSNIHQHEQEIVRYAVEEMKRVQGVTLYGTSDFASRG
                     GVVSFNVDGVHPHDVGSILDEEGIAVRAGHHCCEPFMRKLDISGTVRASFYLYNGPED
                     VDALIRGIKRVKEIFKK"
     gene            complement(11023..>11327)
                     /locus_tag="PKK76_02375"
     CDS             complement(11023..>11327)
                     /locus_tag="PKK76_02375"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HNL67336.1"
                     /translation="RLKVASLSQIKEGEMIAVDTPAGSVAVTRIGNEICAFQNVCTHD
                     DNPLDDGKIEGEEVICPRHGARFNIRTGAVCRMPATSDIEVYPAHLSGEDVFLDID"
BASE COUNT         2881 a         2954 c         2727 g         2765 t
ORIGIN      
        1 cctgtagttc ccgggcaacc gcaggcgaat cgtcttctga aaaggcgaaa acccattttc
       61 gcgcacctgc acttcgagct gaatggaacc gctgcgatcc ttctttcccg atacttcaat
      121 gcgagcattt cgaccgtccg ctgagatccc atcgtccaga atgcattttc cggagatctg
      181 agttcccggg aagatctcaa aggccagagg cgatttcatt gtatcgagtg cattgcgtcc
      241 aactacggcc atgggaaggg ctgtgcccgc acgaacgagc agcggataca taccgggccg
      301 cgtggaatag gtatgacgtc ccgcatcgcg cacaactcct gtgtcaaaat caaaccagcc
      361 ctctcctgca tccggcaggt tcacgcgacg attggccgcc ttgcgttcga aaaccggcgc
      421 tgccagaatg gatgatccca gcaagaactg atcttcgcaa tcctcgtctt tgacattcgg
      481 aaattcatag aacagtggac gaacgatagg gctgccatcc tcgcttgctt catggaatag
      541 attgtatatg tacggtaata gtctatatct acgtcgaatg tgcttgcgac aaattgcaag
      601 gacgctttcg ccaaaactcc atggctcctg atcccgtccg aaaagcacag tgtgcgcacg
      661 aaagaatggc atcaaggatg caagttccat ccatcgtgcg aagagctctg gatcacgtct
      721 ccacttgatg atggccagca ttcctttgcc gcttccaaat ccacctgcgt ccgctcctga
      781 aaacggaaca ccactcaatc cgagatttaa aaccatgtgc aggttttctt tcatctgctg
      841 ccaggaggaa tgattgtcgc ccgtccacat gcatgcgtat ttctgaattc cagagaaccc
      901 ggatctggag agaacgaaag gacgttctcc ctttttaaac ttttcaaatg caagattggt
      961 ggcttctgcc tcgaggttcg cgtacaggtt gcgcatctct gcttgcgaat gatttttgaa
     1021 atgaacgtct tcgagaagcg gatctgtctt cttccccatc ttccatgtgg gctcgttcat
     1081 atcgttccag attcccgaaa ctccgttgcg aaagagaaca gcatgctggt gcgcccacca
     1141 tttgcgcaca ttctcaagag taaaatcggg aaacacagtg gttccaggcc agacttttcc
     1201 tttgaagtag ttgcccatgg aatcacggca gaacgcatct tcatttcttc cgctttcgta
     1261 tgtatcgtac tcttcttcga gtgcaactcc tggatccacg atcgcaacag tcttcactcc
     1321 gagcttatcg agtgattcgt tcattccttt tggatccggg aatgtactct tgtcccaggt
     1381 gaatacttta tatttatcca tgtaatggat atccaggtga atggcatcgc atggtacatc
     1441 gagtgcgcga aagcgttccg caagttccat cactcgcttc tgtgtgcgat agctccatct
     1501 actctggtga tatccaagag cccaaagagg cggaagaaac ggacgacccg taattcgcgc
     1561 ataagcagcc agaacctggc gaaggttccc gctcattacg aacacgtcaa tcggcactgc
     1621 ctctgtagat tgaatatcaa accgaacttc cggtccttcc ggacgagtct ttccttccag
     1681 atcgacttct actctcgtgg ggcggatgca gtttaagaat atgccaatga acgaagagcc
     1741 gcggcgaact acgaagaatg gaaatgcgga ataggaagcc tgatcaatag aatagaaaat
     1801 ggtatcaatg gtcttgagtt caaacgatcc tgctttgacc agtccgcctg tcgcacagcc
     1861 aaaaccaaac actgaatcac caggcatcaa acgcactaca aaggaaggtg gcccaaactt
     1921 tccggtctgc acagtgccgc tcagtctggt ttcgtctggc aaggtgatgt ggaatgagcc
     1981 agtcactgga tcgacaaaca gctttgtgtc gcgcttcttc gccaggatca ctccctgcgc
     2041 atccttgtga atggccagct tttccatttc gactctttcg acaggagccc cttctggaaa
     2101 gtccactaca ggctccggat gaaacaggaa tcgaaacgca tctggtttca acgcggccgc
     2161 aagtatgctg tgagcgtaca ttcgctttat tttcggtccg cgcggccccg aaaatcaatg
     2221 caggcgggct ttttggttgc catggcacgc gcgcaggccc gcctgttgta tgcgcaggaa
     2281 aatcgcctta ttctccactc tgggagtatt tgtcatcctt ggggcggtca tcattcctcg
     2341 tccaggctat cactatcagc ctcattctct ccccgcagac tttgactctt tctatgccat
     2401 gaagctcaag cagagtaagg agaagggtgc tcgtcccggc tgtgaagaaa agctgaatcg
     2461 atttgggcca aaaacagaat atgcaatcct ttatcttcat ggctacggag catccagggc
     2521 agagggagaa gagtcgatcg accgattcgc aaacaaactt cacgcaaata cgtactacgt
     2581 tcgtctgcct ggacacggaa cgaacatgga agaccaggcc tctgtatctt acacagatta
     2641 tctggacgaa agcgaagagg cactacagat gatgcctgcg ctcggagaca aaatcatcat
     2701 cgttggaacc agcatgggcg ctcttctagc cacacatctg gcagcagagc atccggataa
     2761 gctctacgct ctcattattg cttccccttt ctatgagttc gtgaggaaag aggcaaatat
     2821 tgcgagcgtt ccaggcggtg gaaaactcgc agaagtattt ggcggcggaa aaattcgcga
     2881 cgtaactaaa aaaccagacg acgtgaaaga caaacgcagc ccgggctttg aaaactactg
     2941 gtatgaaaaa caatatttct cttccatcca gtctctggca agactcaaaa aagcggtgtc
     3001 cggtgagcac acattcggtc gagtcactgc tcctgtattg atgatctact attacaaaaa
     3061 tgaaaacgaa caggacacag cagcgagcgt tccggctatg ctagaaggat ttcatcaatt
     3121 tggtaagttt accagaccat ctccttacaa ccgctctgtt gcagttgcag acggagatca
     3181 catcatgttt agcaaatgga ttccaacaga caaggatctg attgataaag aactcaatcg
     3241 attcatagac gatctcaaaa aatgaaaact ctcatcatca tccccgcata caacgaagaa
     3301 tcgactattt acgaagtcgt aacaaagact ctcaaacacg cggatgtatc cgtaaccaat
     3361 gacggaagcc gtgatcgcac gccagagatc ctgcgcacca tccaggctga gtgcagcagc
     3421 ggaaagcatg ctcataaact gaacgtagta acgcatgaaa aatcgactca tatcccgaag
     3481 ggcctgcagg acggcttccg ctatggagta gaaaacggat acgatcaatt catcaccatg
     3541 gacgctggaa tgtctcatga tccggacgca atcccggatt tcctcgcggc ggattcgtca
     3601 ctcgatgtgg tgataggcgc gcgcaaacag gtagagaacg tgccttttta cagacgtgtc
     3661 atctccaggt gcgccgctct ggttgtaaac tacgcactca ctccctctta ctttcgcctg
     3721 ggtcctggac tttccgactg caccagtgga tttagacgtt attcgcacag agcgggactt
     3781 ttgatttccg caacggaatt aaaatccaaa gcgtttgatt ttcatatgga agctctggca
     3841 ttgtgcgtgc gcgcaggaat gaaaccgggc gaaattgcaa tccgttatgt cttttccaat
     3901 tcttccttta acagccgcgt tctcaagcag gccatgaaat tcggaatgta cctgatctcc
     3961 acgaaaggaa aggctgcggc atcttgaaat ccgtgaacca ggcaggcctt ctgactgcac
     4021 tgcggtctgt ccgtgcggct ccactcctct tcgcattgta tgcagggact gcttgccttg
     4081 cgattgtcgc gcgatatgat tttgatagca cagcgctcat tcgctttggg catagataca
     4141 tagcgcaaaa ccaggacctg actcctacct ctgccattcg atttcgcggg aacgaagcaa
     4201 atggcggcaa cggatacgat gggcagatct tctactatta tgcgagcacg atgtggaaga
     4261 aaggccagtg gcctgtcggg ttcagcaacg catacagggc cccgcgcgca ggttatccgt
     4321 ttcttgcgtc gttgttttcg tggatgggaa accgcggcac tgcatacggt atgattctgg
     4381 ttcaagtact ctccattgtg ggaggattga tctgcttctg gcacctgctg ccggatgaaa
     4441 gaagaagctg gactctactt cttgcggttt ctccttttgt tttgcagtca tttgcgtttc
     4501 tagtcagcga cgcggtgatg atttcacttg cgatcattgg gatttacttt tcgcgaaaaa
     4561 attctgtggc cgcgagtcta cttgcgtgga tctttttttc cctggctgtt atgaccaagg
     4621 aatcgtcttt gttcatactg ttcccgtacg gactctgggc actgttgcgc aaagactgga
     4681 aattgtgcgt cgttgtggct ggctccctcg tacctatggt ctgctggcag ctgtatctat
     4741 tcagggctca tggcatggtg cctgcaagca ttctgtctac gttcctttct cctctccttg
     4801 gaatcagagg agttgcttcc caaacggcgg atcttttgag aatgaagcct ttcccaatag
     4861 ttgagatctt taaagtatct gccaaatggc ttttgatcct tctcctcccg gtatccgcct
     4921 ggacggctct ccgcggattc aaacttgcag atctacaaaa gatcagcact ccatttagac
     4981 tggccgcgct attcgtggtc ctgagcatct gcattgcgga ttttcagtat ttctggggca
     5041 tctttgaaaa cgtaggaaga atgttcacgg ccctcgtgcc tgcagtgatt ctggccaggt
     5101 cagaaacaaa gacggacaca tcctggaagg cctttgtact tctcctttgc gcattgtctt
     5161 tcctggtgtg ggcccggatt tgttttcttt ctcctgtttt cccgttcgat cattacattc
     5221 catactctgg tccagtctac cctgctcctc cactatgatt ttggttgacg gactcagccg
     5281 caagaaccaa accttcgaag cgtgcatctt aaaggtatga ggaatcaatg agcatctccc
     5341 aggtagacct cagacaaacg cggctcccca tacagcccca gatcatcagc gccctcatgc
     5401 agctgaccga tgattcagaa gttggcttca gagatcttga aaagatcatc aaagcggatg
     5461 aaaacatcag tactctgatt ctaaaagccg ccaactcgcc gctttactcc agggggaaag
     5521 aaatcagatc cattcagatt gcaatttctc tgcttggatt tcgagtgatt cgcacccttg
     5581 caacggttgc cagctcccaa ggaatgtttc agtcagggaa ctacgcacgt tttagaaaat
     5641 atgtgagcga acacgccgtc atcacgggag tgatagcacg aagcatcgcc gtgaaactga
     5701 agttaaagaa tctggaagag gatgcatttg taggaggact cctgcatgac ctgggcaaag
     5761 taatcatgaa ttccctcgat cgatcgcatt acattgaagt gattcacctg gtagagcgag
     5821 gagttccttt tagccaggca gaaaaacaga tctttggttt ctctcatcgc gacattggtg
     5881 aaaaggcggc catgacctgg aatcttccgc agatctaccg atggattctc agcacccacg
     5941 gactcgacgc tctgactccg ccggaaggaa tggacgaatc atcgcgagcg ctcgtgcaga
     6001 ttgttgcatg cgcgaactat ctggcaagac tttatggcta cggcgcgcct ctgtctggaa
     6061 tagaacacga aatctctaaa ttctttgatg ctctaggaat ggattcggat acgcgatcct
     6121 tctacctctc cgagttcaga gagaacattc cgaaagacga attctataac ttcttcatca
     6181 cgatcctata atttatatgc tgcaacaatc gacactcgaa ttcgaaatca gactctacga
     6241 tctcttctcg gaagaaatca gtgctttgcg cgacgcggtc aatcgcctcg cggatgcagt
     6301 ggatcctgtt tcgcttcgcg gacctctcaa cgccattgta tatgagttgt gcggaaatgc
     6361 cctgaaagca ctgtacaaaa gagcctacag caagtacgtt gtggccaaac tcggtctcga
     6421 tgacttaccg tatcatgact ggctgaaatt gtttcgcgca gaaattgagg cgcatcgcgc
     6481 agagaatttc gcgcatctat gcagacagga aaacatttac atcaaggcat ccggaaagtt
     6541 cgcaggggat gtttttagaa ttcaaatcga aaacgacgga gctccatctg aaatcgaaat
     6601 gagtcgcatt cacctcgcac tcgaacgtgc gaaggaaacc atgagcctgg ccgcttactt
     6661 cgatgatgaa gatgcatatc aggaaggcgc gggtctgggg ctcccgatgg tgatggtgag
     6721 tctccgtgga atgggtgtag atcccgccaa ttttcaaatc ttcacgcaag gggacaccac
     6781 agtggcgcgc atagacgtac ctctgaatct attcggcaac gcacgctcag aacctctacg
     6841 gcttctcagg cagaataagg aacttcttgc cattacctgg aatctatttc agcagatcga
     6901 tctatcagcc gtgcgtttcg acctggatgg acacattctg gccgtatcga gaagcatgct
     6961 caagaggctg gccatcccgc tcgaaactcc ggacatgttc aagacgcttg ttcctcagcg
     7021 atttgttgca gaactgtttc gcggtcctca gggaattcga gtcactcaga aattcttaaa
     7081 ccacagagtg tatttggaga acctttcgcg cacagaaaga atcctattca acgttagcgg
     7141 atatgtgacg gacaaaggtg tagtgagtac tctctggcag gaaatcgcta tttccgactc
     7201 tgcggccccc aaactcaacg aaggcagttt gatcgggaac ttaaagtttc acaacattat
     7261 agaaccttat ataccccagc ttgtgttaat gaaggcccgc gagacagtcg agcttggaag
     7321 aacggagctg cccgaagagg tgagagatca gaccatcttc tttgcggacc ttgccggatt
     7381 cacagaaatg tcggagagca tgcgtcctca tgtggtcgtt gaaatgttga atatggctct
     7441 gagcatcatg gtaaggcata tcaaagaagc gggcggtagc gtggacaagt ttatgggaga
     7501 cgctgtcatg gcggttttcg cggatccact tcctgcatgc aaggcagctc tcgaaattca
     7561 gaatacgttc gcagaagtga accgtttcag aatcgaagct aaagagccgc cggtccgggc
     7621 gcgaattggg attcattgcg gcaatgtgat catggcaaac atcggcacag acgaacgcaa
     7681 agactggact gcaattggag acacagtcaa tactgcgtcg cgcattgaga aaaacgcacc
     7741 tgtaggatcc atcttgattt cagacagagt ctttgagcgc gtaaaagatg accttcgagt
     7801 cacgcaaagc tatcgaatca aagtcaaagg gaaagcaaaa gaaatcagcg tatacgaact
     7861 tgaggccatg aactccgccc ctgcagatag ttgatatcct acagatattt gagaaaaaac
     7921 tcggttacct ttgcttccgc ctgagctctg tccgcctgcc aggcgcgtgt atgtctccca
     7981 cccggcacaa cccagtacca ctttggctcc cgagcagcag catagagttc ctctccattg
     8041 tgaggcaaaa ctcgcacgtc cgcagtgccg tgtataatga atactggcct cggcgaaatc
     8101 tccgagatca tcttgaccgg aacaatttct tctgtgttca ttctggcgcg aagggaaacc
     8161 agagccccaa ccaatggcaa aagcggataa cgtggaattt tgtagtcctg ccatgcggcg
     8221 tcggagatcg cggaagagaa agaagcaaat cccccttcaa agatcccggc gcgaattcgc
     8281 aagtcttctg acatggccat gatggacgtc gccgctccca tggaaaaacc aaacactcca
     8341 atcgaatgca tgccgcgcga caccgcgtaa tccaccgctg cgtgcacgtc gcgccgttca
     8401 taatagccca tagaactaaa ggccttctgg ctctggccgc aattcctcag gtcaatatac
     8461 aaaacattga agcccgcaga gtggagtgcg ggcgcaaagc gaagaccttc atgacgatcc
     8521 acgcctcgac catgaactac cacgatcgcc ggagcaccgg cacgtcccgg aatccaccag
     8581 ccgcgaatca tcaaatcgtc ggacgtgcga aactcgatgt tttcaaagcc cagaccaaga
     8641 gaagaaggat ccccgcaata tacaaaatgc tcttccgaac aagcatattt cccggtatag
     8701 agaattgcag aagaaaagta aactgcaccc gcagccagca acgccacggg caagatgacc
     8761 gccaggagcc gcttcatgcg atcccttgca cgcctgcggc ctgcaattca ggtagcaacg
     8821 gctttccggg tggcaaatgg actacggcca tcccaatggc acgcgctccc tcgatattct
     8881 tttccaggtc gtcaatgaag agagtacgtt ctgcagggac gccgagaaga tcaatggctc
     8941 tgtggtaaat gtccggctcc ggtttgcgaa gagcatgttc gtgagagtag atctttgcct
     9001 tgaatatgtg aaagaattta taatttttct caacaaaaga agaatgggac tgatttgtat
     9061 tggatacaag acccacgggc acgcgctgcg aaagcatttc tgccagacga atgttctcat
     9121 tcatggctgt aaagatgtca tcaaaagctt cccggatggt gggtccatct gcagctccat
     9181 ctatctggat agatacatgc tcgaagaatt gctcggggct caacaccccg cgttcaaact
     9241 ccaccatttt ctccgaaaac atggcagatc tgactctgtc cgcagaaacc ccggtgctcc
     9301 tcgatagaac gctgaagcat ttctccaggt caaaatccag caggactttg cccagatcaa
     9361 agatgacggc gtctacactc atcacaccac gttcggaacc tattctttga tcgagaattc
     9421 tttttgcttg cagaaatcta cggccaccgg cttcgaaagt gtaccgttct cgaattttgc
     9481 ctgcacagtc cacagacaat cgccttcctc tgcgaaagtg gcccacacta cggttgcggt
     9541 gatgcctggc ttaattccct ttccaacaaa gagtggaatg cgacgattcg cgcctttctc
     9601 gagtgccgtg agagtcgtaa ttttctgagc cgtttcgttg gtaacgtcaa attgaatttc
     9661 gcgagccagg agagctgttg taaaccctgc aaagattgcg atgaacaaga aagttcgcat
     9721 tggagattcc cccgttttga tgtgatggtt caaataacga actatcaggc caggccttca
     9781 tgttgaaaac attatttctt aaagatttct ttgactctct tgattccgcg aatgagcgca
     9841 tccacgtcct ccggcccatt gtacagatag aagctggcgc gaaccgtccc actgatgtcc
     9901 agctttcgca taaagggttc gcaacaatga tgacccgcgc gaaccgcgat tccttcctca
     9961 tccaggatgc tgccgacgtc atgaggatgc actccgtcta cattgaaaga aacaacgcct
    10021 ccgcgcgaag cgaaatcaga cgtgccgtac agggtaaccc cctggactcg tttcatctct
    10081 tctacagcat aacgaactat ttcttgctca tgctggtgaa tattggagag acccgcctgc
    10141 ctgagatact cgagcgcgat agcaaaagca actacgcctg cgatgttcgg agtgcccgct
    10201 tcaaatttca tgggaagttc tgccggaaga tacgaatcct tctgtactgt gaggatcata
    10261 tctccgccac ccagaaaggg atccatgcct tcgagaagct tccgcttgcc atagaggacc
    10321 ccgacacccg ttggaccaag catcttgtga gcgctgagag aatagaaatc gcaatctatc
    10381 tttcgcacat ccaccggtaa atgacaggcg ccctgcgcac catccacaga gaacagcgca
    10441 cctacagcat gagctttttt tgcaaggagc tcaatgtcgt gtatcgtccc tactgcattg
    10501 gacatatgcg ccagggcgac catgcgagtt ttcggagaaa gcacagaatc gatcttagaa
    10561 tagtcgtact tatggtcttc tgtaagtggt aaatgcttca gtacggcacc agttttcttt
    10621 gcggcaatca tccacggcac gagattggaa tgatgctcca gctctgaaac aagcacttca
    10681 tcccccggct tcaaattgct cagaccccag ctataggcta caaggttgat gctttccgtc
    10741 gtgttgcgcg tgaagatgat ttcatccgga ctctctgctc caagaaaagc agccatcttc
    10801 ttgcgtgtcc catcataaag gtctgtggct tcataagata gacgataggc gcccctgtga
    10861 atgtttgcgt tgcgacagcg atagtaatct tcgaacgcgt caatgactct ctgcggcttt
    10921 tgggaactgg ccgcgctgtc caggaaaacc aggggctttc cattcatcat agtttgcagg
    10981 attggaaagt cctgccggat gcttttgtaa tctagagcca tatcaatcga tgtccagaaa
    11041 gacatcttct cctgaaaggt gcgcggggta aacttcgata tcggacgtcg cgggcattct
    11101 gcaaaccgct ccagtgcgaa tgttgaaacg cgcaccatgt cttgggcaaa tcacttcttc
    11161 gccttcaatc ttgccgtcat cgagcggatt atcgtcatgc gtacacacat tttgaaaggc
    11221 acatatttca ttgccaatac gcgtaacggc aacggagccc gccggggtat ccacggcgat
    11281 catttctcct tctttgatct gcgaaagcga tgcaacctta agtcgac
//
LOCUS       DAORFF010000031        11260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2592_length_11260_cov_3.881124, whole genome shotgun sequence.
ACCESSION   DAORFF010000031 DAORFF010000000
VERSION     DAORFF010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11260
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2592_length_11260_cov_3.881124"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1944)
                     /locus_tag="PKK76_02380"
     CDS             complement(<1..1944)
                     /locus_tag="PKK76_02380"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNL67337.1"
                     /translation="MPSLSTRYEPASIESRWYEVWEESGVFRPESTGKGEPYCIVIPP
                     PNVTGQLHVGHALNHSIQDVLIRHARKKGKRTLWVPGTDHAGIATQAVVEKLLKKEGT
                     DRFQLGREKFVKRVWEWKDKFGNIITKQMRTLGLSVDWERERFTMDDGLSRAVREMFV
                     ELYKKKLIYRDTRMIMWDPVARTVLSDIEVEYEDNYPGELYSFAYKLSQGDGEIVVAT
                     TRPETMLGDTAIAVHPEDPRFKKLIGKTVKHPFLDREIVIIADSILVDMEFGTGAVKV
                     TPAHDMNDYETGKRHNLESITIFDEAGRINSKGGSFVGLDRFEARKQIKAQLKERGLE
                     RGVKEHKMAIGKSQRSGAIVEPMVSTQWFVSTKPLAKKAIEVVEKGKIRFYPENYANL
                     YNSWMKEIRDWCISRQLWWGHRIPAYHCEDCGHITASVDPPKACEKCKKKNLIQDPDV
                     LDTWFSSGLWPFSTLGWPAKTKDLATFYPTSVLVTSYDIIFFWVARMVMLGLFTMKKI
                     PFHHVYIHGLMRDERGIKISKSLGNNIDPADVIANYGLDAYRFFLMATLSEGKDSTYS
                     ETRLKGYQAFANKVWNSSRFVIMNLPEDFAPVDLAAIVFAKPSRDAKKSTKKSPQKQT
                     LELEQEDWWILSRLAETLTAMDKN"
     gene            complement(1990..2247)
                     /locus_tag="PKK76_02385"
     CDS             complement(1990..2247)
                     /locus_tag="PKK76_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67338.1"
                     /translation="MRALIAIALLSLAGCQKIKSLWSHSESKIDPCDSVSNYYEGDRH
                     FVERDFVTQDRNLDYRKLFEADKIQNSPCDPVHGRIQKTEH"
     gene            complement(2244..2435)
                     /locus_tag="PKK76_02390"
     CDS             complement(2244..2435)
                     /locus_tag="PKK76_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67339.1"
                     /translation="MNLSSVPGVFAYKIQRYPEQPVQPVERTELSLPERSRRFEMPRL
                     YGPDAQAPDSPGHLIDLFA"
     gene            complement(2499..2876)
                     /gene="apaG"
                     /locus_tag="PKK76_02395"
     CDS             complement(2499..2876)
                     /gene="apaG"
                     /locus_tag="PKK76_02395"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNL67340.1"
                     /translation="MAPVQEIQVKAVARYMPERSNPLEPIYFFSYNITITNQGNLSAR
                     LQSRYWHITDATGRVQEVRGPGVVGEFPNLKPGESFNYTSFCPLSTNYGFMQGHYTME
                     REDGSTFDAPIPPFALAVPDSIN"
     gene            2990..5686
                     /gene="ppdK"
                     /locus_tag="PKK76_02400"
     CDS             2990..5686
                     /gene="ppdK"
                     /locus_tag="PKK76_02400"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNL67341.1"
                     /translation="MSQYVYFFGEGRTDAPALDKMLLGGKGANLCEMSQLGIPVPPGI
                     VITTDACKRYVRDNRFPTGLLEEVLVRVQQLNSATGKVFGNPDRPLLVSVRSGAPVSM
                     PGMMDTILNLGLNSETLEGMVRSTGNERFVCDSYRRFVQMYGDVVLGMEHHDFEHILN
                     DVKKSVGAASDTDLRAGDLREIIRQYKDLVRRITGNDFPEDVHEQLRGAIEAVFKSWN
                     NDRANIYRRLNHISDDLGTAVTVQSMVFGNMGDTSGTGVAFTRNPSTGERKFYGEYLM
                     NAQGEDVVAGIRTPYPIGRLGDDMPEVFGQLDEVQKKLEAHYHDMQDLEFTIQEKKLY
                     MLQTRSGKRTGVAALRMAVEMVDEGLIDRKTALKRVDPESIPSMLAPVFDPEEKKKAI
                     KEKRYLTRGLNAGPGAASGIVVFDSWNAQNRKIKGEKTILVRMETSPEDIAGMEAAEG
                     ILTARGGMTSHAAVVARGMNKPCIVGCSSLHVEERSGFMAVATEDGRSIIVREGESIS
                     IDGSTGEVMLGHLPTRPSEIQQVLDGLVPEETSMVAKYFKKFMSWTDEVRTLQIRTNA
                     DTPRDARIARTFGAQGIGLCRTEHMFFEGDRIAAMQEMILSDTVSAREKALEKLLPYQ
                     REDFIGIFEAMEGLPVTIRLLDPPLHEFIPHDEGHMGELAHKSGVSLEKIRVRVEALK
                     EQNPMLGHRGCRLGVTYPEITKMQVRAILEAAVTVKKKGKSVYPEIMIPLVGHIKELR
                     NQKELIEATAQLVMRESNTRVDYLIGTMIEVPRAAIVADEIAQEAKFFSFGTNDLTQM
                     AMGFSRDDADKFLRDYLRLGFYERNPFQSIDQEGVGALVKMAIERGKKTRPDIKLGVC
                     GEHGGDPTSIGFFHNAGLHYVSCSPFRVAVARAAAARAALGV"
     gene            complement(5745..6773)
                     /locus_tag="PKK76_02405"
     CDS             complement(5745..6773)
                     /locus_tag="PKK76_02405"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNL67342.1"
                     /translation="MSRFVAFISFLSIGSCYLAHAARGQASLLLGRQNIDDVIHSPDT
                     DENTRKKLEHVLAVRSWAHDKLGLSTNSFKKFTKLDRRAVAWNVSASRELRFEPHTWW
                     FPIIGTVPYLGFFEESKANAEADKLRREGWEAIVTEVAGYSTLGWFEDPLVSPQLEYS
                     EWGLTRLVIHECTHATVWLPGSVDFNESFASFVELEGAMTYVRDKYGVESEEYKKRIL
                     QEEESSRVRFLFHKLAQSLNTIYESNATDEEKRNRKRETIAAFRTTLLSKIPEFKAVN
                     MRAVAERDLNNAHMLSFLRYESGEEYFRDQFKECNAQWPCFLDRMKSLKKEPSGWKTS
                     RKVPDSGQ"
     gene            6852..7250
                     /locus_tag="PKK76_02410"
     CDS             6852..7250
                     /locus_tag="PKK76_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67343.1"
                     /translation="MRFFAAVVMGFSLATVGLRADPVLLRAVQIDAQPDEVLRKLAAA
                     LNQDLKTVLESKGLQVTTQGAAEAAKPALRGIKECKNAECARTIYDQAGYTQILVPIL
                     QRNKGSENLLCYLYRVDGDRITDENLLILL"
     gene            7292..8248
                     /locus_tag="PKK76_02415"
     CDS             7292..8248
                     /locus_tag="PKK76_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67344.1"
                     /translation="MVQFFPDATGPVAEVKPPEPEKKPEEKKPEPEIARKPEETKDQT
                     RRDQKELNPEQSPKSEREPERTGPSLIRIASNPVAEVFIDGTRVGTTPFEMPADGTLK
                     TVRLTAKDHDDLVVPLRSMKSMEIMLTLRRIRDYAPPSRSDVSPLGALARSLVLPGWG
                     QYASGNQFSSYLFGTGSALALSTALYSAKDMRQSRTKIVEQQRNFDLYVYAYGSGSTN
                     LFLTQAGFASFASRDAFFENNLTNCSSRECSHYRQAKSIHRNALGAFAILYLWNAVDA
                     FLSAPSFPRETKSLSLFASPVLAMSERGIERGIQFGATVRFQ"
     gene            8245..9096
                     /locus_tag="PKK76_02420"
     CDS             8245..9096
                     /locus_tag="PKK76_02420"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNL67345.1"
                     /translation="MRSLLKCLLPLVLAVVAGGLSAETRRIRIVTEPTGADVYVDGVL
                     AGKTPLDVETDGRLKNIRIVSEGFREISFPLRADGGAELVFGLRKAMQTLPDERSSGG
                     LNFLAMARSAILPGWGQYSKGDGSAFLFGAGTIFAGGLLYQTHLDVDVRRRAAVNGFL
                     RGDYYSLAYYQAQALTNSNLGPTFNASAVILASDLMPSRQDTLINSCVPYSTFQMFTV
                     SKGACDRFHRARRNYHRAKYAFAGMYLWNVLDALIADPRSSVSFSSSPVLSDEEKGVY
                     LGAVVRF"
     gene            9213..10370
                     /gene="metK"
                     /locus_tag="PKK76_02425"
     CDS             9213..10370
                     /gene="metK"
                     /locus_tag="PKK76_02425"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNL67346.1"
                     /translation="MFLKDFLFTSESVSEGHPDKICDQISDAVLDAHLAQDPASRVAC
                     ESLVTTDFVCVAGEITSNAKVDVEALARKVINKIGYTTNDIGFCADTAQVMVRLHSQS
                     PDISMGVTEGEGLYKEQGAGDQGLMFGFANDETPELMPLPISLAHRLMKKLADLRHSG
                     EFKDILPDSKSQVTVEYRDGKPSRIDTVVISTQHREHLEHKVLTEMLVEQVIKKVIPA
                     NYLTNTRYLINPTGKFVVGGPHGDCGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDR
                     SAAYMARYVAKNVVAAGLCTKCEVQVSYAIGFPQPTSILVDSFGTGKIPDEKIAERVK
                     SVFNFSPGAIEKLLKLRDKGRTYLDTAAYGHFGRDGETFTWEKTDKAEALK"
     gene            10374..>11260
                     /locus_tag="PKK76_02430"
     CDS             10374..>11260
                     /locus_tag="PKK76_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNL67347.1"
                     /translation="MAARKKKAARKPVKVATKSRGKPASKKPAKKAGVAKGQSAKAAP
                     VVEVVKKATRDGYGEALAELGKTRPEVVVLDADLSESTRTHKFARAYPDRFFNVGVAE
                     QNLVGMAAGLSLSGLVPFASSFAMFLSGRAWEIVRNSVAYPGLNVKLVASHGGITVGE
                     DGASHQCIEDFAVMRAIPKMRVFVPSDFTEAKQMIAAVAAMKGPCYIRVSRAATPVLS
                     RPPSYKFQEGKGELRRDGKDVTIIACGVMVAEADLAAEKLAKQGIQAAVINMASIKPI
                     DEKLVLAYAKKTGAIVTSE"
BASE COUNT         2652 a         2768 c         2984 g         2856 t
ORIGIN      
        1 gtttttgtcc atcgctgtga gcgtttctgc gagtctcgat agaatccacc aatcttcctg
       61 ttccagttcg agtgtttgtt tttgaggaga tttctttgtg gatttttttg catctctgga
      121 tggtttcgca aagacaatgg cagccagatc cacaggcgcg aagtcttctg gcaggttcat
      181 gatcacgaat cggcttgaat tccacacctt gttcgcgaag gcctggtagc ctttgagtct
      241 ggtttcagaa tacgtagagt ctttcccttc cgaaagcgtg gccatcagaa aaaagcgata
      301 cgcgtccagt ccatagttag cgatcacgtc tgccggatcg atgttgttcc cgagactttt
      361 tgaaatcttt atgccccgtt catctcgcat cagtccatga atgtacacat gatgaaacgg
      421 aattttcttc attgtgaaga gaccgagcat caccatgcga gcaacccaga agaagatgat
      481 gtcataggat gtgacaagca cggaagtcgg gtaaaatgta gccaggtctt ttgtttttgc
      541 gggccatcca agagtggaga aaggccagag accggaggag aaccatgtgt cgagcacgtc
      601 cggatcttgg attagattct tctttttaca tttttcgcat gcctttggcg gatccacaga
      661 ggcagtgatg tgaccgcagt cttcgcaatg atatgccggg attcgatggc cccaccagag
      721 ctggcgagag atacaccagt ctctgatttc tttcatccat gaattgtata gatttgcata
      781 gttttctgga tagaaacgga tcttgccctt ttccaccact tcaatcgcct tctttgccag
      841 aggcttagtg cttacgaacc actgagtgga aaccataggt tccacaatcg ctcccgagcg
      901 ctgtgacttg ccgattgcca ttttgtgttc tttgaccccg cgctcaagtc cgcgttcttt
      961 tagttgcgct ttgatttgct ttcgggcttc gaaacgatca aggcctacaa acgatccgcc
     1021 tttcgaattg attcttcctg cctcatcgaa gatggtgatg gattccagat tgtggcgctt
     1081 tcctgtttcg tagtcgttca tgtcgtgtgc gggtgttacc ttgacggcgc ccgttccgaa
     1141 ttccatatct acgaggatac tgtctgcgat gatgactatt tcgcgatcga ggaaaggatg
     1201 ctttactgtt ttgccaatga gcttcttaaa gcgagggtcc tctggatgca cagcaatggc
     1261 cgtgtccccg agcatcgttt ctggtctcgt ggtagccacg acgatttcgc catctccctg
     1321 gctcagtttg tacgcgaatg aatacagttc gccggggtag ttgtcttcgt attcaacttc
     1381 tatgtctgag agaaccgtgc gggcaactgg atcccacatg atcattcttg tgtcgcgata
     1441 gatcagtttc ttcttgtaga gctctacaaa catttcgcgc actgcgcgcg aaagtccatc
     1501 gtccattgtg aaccgctcgc gttcccagtc cacgctcagt ccaagcgttc gcatttgctt
     1561 tgtgatgatg tttccgaatt tgtctttcca ttcccagact cgttttacga atttttcgcg
     1621 accaagctgg aatcggtctg tgccttcttt cttcagtaat ttctcaacca ctgcctgagt
     1681 tgcgatacca gcgtgatccg ttcccggtac ccacagcgta cgcttccctt tttttcgcgc
     1741 atgacgaatg agtacgtcct ggatggaatg attgagtgcg tgtcctacgt gcagctgccc
     1801 cgtcacattg ggcggtggaa tgacgatgca gtaaggttca cctttaccgg ttgattccgg
     1861 gcgaaagacg ccagactctt cccagacttc gtaccagcgg ctttcgatgc ttgcaggttc
     1921 gtatcgcgtg ctcaggcttg gcatgcgcca ctttgcgtgt ttttgcctgc gtgaaaataa
     1981 gaaatctaat cagtgctcgg tcttctggat gcggccatga accgggtcgc aaggagaatt
     2041 ctggatcttg tctgcttcaa atagtttgcg ataatcgagg tttctgtcct gagtcacaaa
     2101 atctctttct acgaagtgcc tgtccccttc gtagtaattt gagaccgaat cgcatggatc
     2161 gattttgctt tcgctgtggc tccagaggct tttgattttt tgacaccccg ccagcgacag
     2221 cagggcgata gcgatgaggg ctctcatgca aacaggtcaa tcaggtgacc ggggctgtcc
     2281 ggtgcctgcg cgtctggtcc atacagcctg ggcatttcga atcgtctgct gcgttcgggc
     2341 agggacaatt ccgtccgttc cacgggttgt actggctgtt ccgggtagcg ctggatcttg
     2401 tacgcaaata cgcccggaac gctggacaga ttcatggagc ctgattcgac cctcatgctt
     2461 tatctatcgg caggaactgg tttgggagtt agacgagcct agttgatgct gtccggaact
     2521 gccagggcga agggaggaat cggggcatca aaggtggaac cgtcctctct ttccatagta
     2581 tagtgtcctt gcatgaaccc ataattggtc gagagagggc aaaagctggt ataattgaaa
     2641 gattctcccg gttttagatt cgggaattcc cccaccactc ccggaccccg aacttcctgc
     2701 acacggccgg tcgcatccgt gatgtgccag taacgggatt gaaggcgagc gctcagattg
     2761 ccctggtttg tgatggttat attataggag aagaagtaga tgggctcgag cgggttggac
     2821 cgttcgggca tgtaacgggc tactgccttt acctgaattt cctgtacggg agccatcgcc
     2881 cggaattgtc tttgaccccg gcccggcgct caagcgtttt ttcacgttgg cttgacagtt
     2941 ccgattcgac gcccactctt cagcctcacg aatatcttcg aggggattta tgtcacaata
     3001 cgtttacttt tttggcgagg gccgtacgga tgctccggct ctcgataaga tgctgcttgg
     3061 cggcaagggc gcgaatctct gtgagatgtc tcagctcgga attccagttc cgccgggcat
     3121 tgtcattact acggacgcat gcaaacgcta tgtacgcgac aaccgtttcc ccactggcct
     3181 cctcgaagaa gttctcgttc gcgttcagca gctgaattct gcgacaggaa aagtttttgg
     3241 aaatccggat agaccactgc ttgtgtccgt tcgctccgga gctcctgtgt ccatgcctgg
     3301 aatgatggat acaattctaa acctcggcct gaattcggag actctcgaag gaatggtgcg
     3361 atctactggc aatgaaagat tcgtttgtga ttcgtatcgt cgtttcgtgc aaatgtacgg
     3421 agacgttgta ctgggaatgg agcatcatga ctttgaacac atcctcaacg atgtgaaaaa
     3481 aagcgtgggc gcggccagcg atacagatct tcgcgcggga gatctgagag aaatcattag
     3541 acagtacaag gacctggttc gccgcataac gggaaatgat ttccctgaag acgtgcacga
     3601 acagctgcgt ggagccatcg aggcagtatt caaatcctgg aacaatgacc gtgcgaatat
     3661 ttatcgcagg ttgaatcata tttctgatga tctgggtacc gcggttaccg ttcagtccat
     3721 ggtgttcggg aatatgggag atacgtccgg cactggcgtt gcatttacgc gaaatccttc
     3781 cacaggcgaa aggaagtttt acggcgaata cttaatgaat gctcagggag aagacgtggt
     3841 agccggcatt cgcacgcctt atccaatcgg gcggctcgga gacgatatgc cagaagtctt
     3901 tggccagcta gatgaagttc aaaagaaact agaagcgcac taccatgaca tgcaggatct
     3961 tgaatttacg attcaggaga agaaactcta catgctccag accaggagcg gaaaacgcac
     4021 tggcgtggct gcacttcgca tggcagttga aatggtggat gaggggctaa tcgatcgcaa
     4081 gacggcactg aagagagtag atccagaatc gattccatcg atgcttgctc ctgtattcga
     4141 tccagaggaa aagaaaaaag cgatcaagga aaagcgatat ctgactcgtg ggctgaacgc
     4201 cggtccagga gctgcatcag gaattgtagt gtttgattcc tggaatgcac aaaatagaaa
     4261 gatcaaaggt gaaaagacca ttctcgtacg catggaaacc agccccgaag acatcgctgg
     4321 catggaagcg gcagaaggaa ttctaactgc tcgcggtggg atgacttcgc acgcagcggt
     4381 tgtcgctcgc ggtatgaata aaccctgtat cgtgggttgc tcttctctac atgtggaaga
     4441 gagatctggt tttatggccg ttgcaacaga agatggcaga tccatcatcg tgcgcgaagg
     4501 ggagagcatc tccattgatg gaagcacagg agaagtgatg ctcgggcatt tgcccacaag
     4561 accttccgaa atccagcagg ttcttgatgg ccttgtgccg gaagaaactt ccatggttgc
     4621 aaaatacttt aagaagttta tgtcctggac agacgaagtt cggacgctgc agattcgcac
     4681 aaacgcagat actcctcggg atgcgcgcat tgctcgcacg ttcggggcgc agggcattgg
     4741 tctttgcaga acggagcaca tgtttttcga aggcgaccgc attgccgcca tgcaggaaat
     4801 gattctctcg gacacagtga gtgctcgcga aaaggctctg gagaaactac ttccatatca
     4861 aagagaagac ttcatcggaa tatttgaggc aatggaaggt cttcctgtga ccattcgtct
     4921 actcgatcca cctctccatg agttcattcc tcacgacgaa ggacacatgg gagagcttgc
     4981 gcacaaaagt ggtgtgtcac tcgagaagat tcgcgttcgt gtagaggccc tgaaagaaca
     5041 aaacccgatg ctcggacatc gcggttgccg cctgggtgtg acgtatcctg aaatcactaa
     5101 gatgcaggtg cgtgcgattc tggaagctgc cgtcactgtg aagaagaaag gcaagagcgt
     5161 gtatcccgag attatgattc cgctcgtggg acacatcaag gaactacgca atcaaaagga
     5221 actcatcgaa gctacagccc aacttgtgat gagagagtct aatactcgag tagactattt
     5281 gatcggaacc atgatcgaag ttccgcgtgc ggctatcgtt gcagatgaaa ttgctcagga
     5341 agcaaaattt ttcagcttcg ggaccaatga cctgacgcaa atggccatgg gattttcgcg
     5401 tgatgatgca gacaagttcc tgcgcgacta tcttcgcctt ggtttctatg agagaaatcc
     5461 gttccagtct atcgaccagg aaggcgtagg cgcactggta aaaatggcaa tcgaacgcgg
     5521 taaaaagacc aggccggaca tcaaactggg tgtttgcggc gagcacggtg gagatcctac
     5581 gagcattggt ttcttccaca atgcgggtct gcattacgtt agctgctcac cgttccgtgt
     5641 tgcggtggca agagccgccg ccgcccgcgc agctctcggc gtttaggtca aaggctctcc
     5701 ggccgcgaag aaaatggaag aaatcgactt cgcggtcgcg tgactcattg cccggagtct
     5761 ggaacctttc tagaggtttt ccatcccgat ggttcttttt tcaaactctt catgcgatcc
     5821 agaaagcacg gccactgtgc attacattct ttgaactggt cgcgaaagta ttcttctccg
     5881 gattcatatc gaagaaagct cagcatgtga gcgttgttca ggtctcgctc ggcgactgcg
     5941 cgcatattga ctgccttgaa ctctgggatt ttggacagaa gcgtggtgcg aaaggcggct
     6001 atcgtttcac gtttccgatt tcttttttct tcatccgttg cgttagactc gtagatagta
     6061 tttagtgatt gcgcgagctt atgaaacaag aatcgcacgc gggacgattc ttcctcttgc
     6121 agaattctct ttttgtactc ttcggactcc actccatatt tgtcgcgaac gtaagtcatg
     6181 gcaccttcca gttcaacaaa agaagcgaat gattcattga aatccacgct gccaggtaac
     6241 cagacagttg catgggtgca ttcatggatt acaaggcgcg taagacccca ttcggagtat
     6301 tccagctgcg gggagaccag cggatcctca aaccatccga gcgtagaata tccggcaact
     6361 tctgttacaa tggcttccca tccttcgcgc cttagcttgt ctgcttccgc gtttgctttt
     6421 gattcttcaa aaaaacccag atacggaacg gtgccaatga tgggaaacca ccatgtatgc
     6481 ggctcgaacc ttagttcgcg cgaagctgag acgttccacg ccacggcgcg tctgtccaat
     6541 ttggtaaatt ttttaaagga atttgtgctg agtccgagtt tgtcatgcgc ccaggatcgc
     6601 acggcaagca catgctccag tttctttcgc gtgttttcgt ctgtgtcggg tgaatggatt
     6661 acgtcgtcta tgttctgtcg tccgagcaaa agagacgcct gaccccgggc ggcgtgcgcg
     6721 agatagcaac taccgatgct caaaaatgaa atgaacgcta cgaaacggga catgaacatc
     6781 atctatcggg caaaaggtcg ttttttcgtc aaatagtttg accgagcgtg ttgtttcgtc
     6841 gtaggtgggt tatgcgcttc tttgccgcag tcgtcatggg tttttcttta gctaccgttg
     6901 gccttcgggc tgatccagtg cttcttcgtg cggtgcagat tgatgcccaa ccggatgaag
     6961 ttcttcgcaa actcgcagct gctttgaatc aggacctgaa gactgttctt gaaagcaaag
     7021 gtcttcaagt aaccacccag ggagcggctg aggcagccaa acctgcactt cgcggcatca
     7081 aggaatgcaa gaacgcggag tgtgcgcgga ctatatacga tcaagcagga tatacgcaaa
     7141 ttcttgtccc catcttgcag cgaaacaaag gtagcgagaa cttactgtgt tatttatatc
     7201 gagtggatgg tgatcgcata acggatgaga atctactgat acttttataa atttgccgcg
     7261 agatgagcgt cgtgccgcga ttgcatccgt gatggttcag ttttttcccg atgccaccgg
     7321 accggtagcc gaggttaagc caccggaacc cgagaagaaa ccggaggaaa aaaagcccga
     7381 gccggagatc gcgcgcaaac cagaagaaac aaaagatcag acccggcgag atcagaaaga
     7441 actgaatcca gagcaatcgc cgaaatcgga aagagagcca gagcgcaccg gaccatcttt
     7501 gattcgcatt gcaagcaatc cagtcgcaga ggtattcatt gatgggacca gagtagggac
     7561 tactcccttt gaaatgcccg cagacggcac tttgaaaacg gtgcgtctta ccgcaaagga
     7621 tcatgacgac ctggtagttc ctctgcgatc catgaagtcc atggaaatta tgttaacgct
     7681 gcggcgaatc agggattacg cgcctccttc caggtcggac gtttcaccgt taggcgctct
     7741 tgctcgctct ctggtgcttc ctggctgggg ccagtacgcg tcgggcaatc aattttcttc
     7801 gtatttgttt ggcaccggaa gcgctcttgc cttatccact gccctttaca gcgcaaaaga
     7861 catgcgacaa tctagaacga aaatcgtaga acaacaacgg aattttgact tatacgttta
     7921 cgcctacgga tccggttcca ccaatttatt cctgacgcag gccggattcg caagctttgc
     7981 gagccgagat gcttttttcg agaataatct tacaaattgt tcttcgcgcg aatgctctca
     8041 ttacaggcag gcaaagagca ttcacagaaa cgcactgggc gcgtttgcca ttttgtattt
     8101 gtggaatgcg gtcgatgcgt ttttgagtgc tccttcgttt cctcgcgaaa ccaaaagcct
     8161 ttctctgttt gcttccccgg tcctcgccat gtctgaacgc ggaattgagc gtggaattca
     8221 atttggcgct acggtgaggt tccaatgaga tcacttttga aatgtcttct tcccctggtc
     8281 ctggcagttg tcgcaggcgg tctctcggca gaaacgcgcc gcattcgtat tgttacagaa
     8341 ccgaccggtg cggatgtgta tgtggacggt gtgcttgcgg gaaagacacc actggatgtt
     8401 gaaacggacg gaagactgaa aaacatacgc atcgtgtccg aaggttttag agaaatttca
     8461 tttcctttac gagcagatgg tggcgcagag cttgtatttg gactgcgaaa ggcaatgcag
     8521 actctaccgg atgagagatc gtccggcggc ctgaattttc tggccatggc gcgctctgcc
     8581 attctgcccg gatggggtca gtactctaaa ggggacggct ctgcgttttt gttcggtgcc
     8641 ggcacgatct tcgccggcgg tctgttgtat cagacgcatc ttgatgtgga tgtgcggcga
     8701 agagccgcgg tcaatggctt tctcagaggg gactattatt cactggcata ctaccaggcg
     8761 caggcgctca ccaacagcaa tctcggcccg acctttaacg ccagcgcggt catcctcgcg
     8821 tctgatttaa tgccgtcccg gcaggacacc ttgatcaata gttgcgtacc ttactcgacg
     8881 tttcaaatgt tcacggtatc caagggagcc tgcgatcgct ttcatagagc caggcgaaac
     8941 tatcaccggg caaagtatgc ctttgccggc atgtatctat ggaatgttct agacgctttg
     9001 attgcggacc ccaggtccag cgtctccttt tcgagcagcc cggtcctttc ggatgaggaa
     9061 aaaggggtct atctgggagc agtcgttcgt ttttgagggc atcgcaggga aatggccgca
     9121 cgtttcctca atgctctttt cattgaccca ccggccgctc ccaaaaagct gatcattccg
     9181 cttgaagcgg tctatcagga gaacccatgg ccatcttttt gaaagacttt ctcttcacct
     9241 ctgagtccgt atcagaaggg catccagaca agatctgtga ccagatttct gacgcagtac
     9301 tcgatgcgca tcttgcgcaa gatccggcaa gccgcgtagc ttgcgaaagt cttgttacta
     9361 cagacttcgt gtgtgttgca ggtgaaatta cgtcaaatgc caaagtggac gtagaagccc
     9421 tcgcgcgcaa agtgatcaac aaaatcggtt acacgacaaa cgatattggt ttttgcgcag
     9481 atacggcgca agtgatggtt cgcctgcatt ctcaatcgcc tgacatttct atgggtgtta
     9541 cggaagggga aggtctctac aaagaacagg gagccggcga ccagggtctg atgttcggat
     9601 ttgcaaacga tgaaacgcca gagcttatgc ctctgcccat ctctcttgct caccgactca
     9661 tgaagaagct cgcggatctt cgccattcag gcgaattcaa agacattttg ccagactcca
     9721 aatctcaggt cactgttgaa tatcgcgacg ggaaaccttc tcgcattgat actgtggtca
     9781 tttctactca gcaccgcgaa cacctggagc acaaggtgct tacagaaatg ctcgtggagc
     9841 aagtgatcaa gaaagtgatc cctgcaaact atctgactaa cactcgctac ctcatcaacc
     9901 cgactggtaa gttcgtagtc ggtggtcctc acggagactg tggtttgacc ggtcgtaaga
     9961 tcattgtaga tacctacggt gggtatggtc gtcacggtgg tggcgctttc tccggaaaag
    10021 atccaacaaa agtagaccgc agcgccgcct acatggcgcg ctacgtggcc aaaaacgtgg
    10081 tggcggctgg tctttgcaca aaatgcgaag ttcaagtttc ctacgccatt ggatttcctc
    10141 agccaacttc cattcttgta gattcttttg gcacaggcaa gattcctgat gagaagattg
    10201 cggagcgcgt gaagtccgta ttcaatttct ctccaggtgc catcgaaaag cttctgaagc
    10261 ttcgcgacaa aggacgtacg tatctggaca cagcagccta tggtcacttt ggtcgcgacg
    10321 gcgaaacctt cacctgggaa aagacagaca aagcagaggc cctgaagtaa tctgtggctg
    10381 cccggaagaa aaaagctgcg cgcaaacctg ttaaagtagc aacgaaatcc aggggaaagc
    10441 ctgccagtaa gaagccggca aaaaaagctg gcgtcgcaaa agggcaaagc gcgaaagctg
    10501 cgcccgttgt tgaagttgta aaaaaagcaa ctcgagacgg ttacggagaa gcacttgcgg
    10561 aactcggcaa gactcggcct gaggttgtgg ttcttgacgc agatctgtct gaatctactc
    10621 gcactcacaa attcgcgcgc gcgtatccgg atcgtttttt caacgtagga gtcgcggagc
    10681 aaaacctcgt tggaatggct gccgggcttt ctctttcggg actggtccca ttcgcttcga
    10741 gctttgctat gtttctttcc ggtcgcgcct gggaaattgt ccgtaactct gtcgcttatc
    10801 ccggcttgaa cgtaaagctg gttgcatctc acgggggcat cactgttggt gaggatggtg
    10861 caagccacca gtgcatcgaa gattttgcag tcatgcgcgc gatcccaaag atgcgcgtat
    10921 tcgttccttc cgatttcacg gaggccaagc agatgatcgc agcggttgct gcgatgaagg
    10981 gcccatgtta tattcgggtt tcgcgcgcgg caactcccgt gctttcgcgc ccaccttcct
    11041 ataagtttca ggagggaaag ggagagctca gacgagacgg taaggatgtt acgatcattg
    11101 cctgtggcgt gatggtcgcg gaagcggatc tagctgcaga aaaactggcg aagcaaggaa
    11161 ttcaggctgc cgtgatcaat atggccagca tcaagccaat tgatgagaag ctggttctgg
    11221 cctacgcgaa gaagacaggt gcgattgtaa cttccgaaga
//
LOCUS       DAORFF010000032        11238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2601_length_11238_cov_4.013950, whole genome shotgun sequence.
ACCESSION   DAORFF010000032 DAORFF010000000
VERSION     DAORFF010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2601_length_11238_cov_4.013950"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKK76_02435"
     CDS             <1..180
                     /locus_tag="PKK76_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67348.1"
                     /translation="MLTSLTEQKRATREIESTIGNVATTAQTVSSGTHELRTLATQLR
                     EKSEELLGTASRFQI"
     gene            complement(177..1208)
                     /locus_tag="PKK76_02440"
     CDS             complement(177..1208)
                     /locus_tag="PKK76_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNL67349.1"
                     /translation="MITILIDIAWILAAAAGIVALRSFLRNPSLKDARDMLASRLRVR
                     IAFYVVCAYLGVGLIGQIKVPEKERIADRSLLDLSLQWIQKERTYSAPFASEVTGIRM
                     LEADRELNRVKGIHILGTDINGVDVMHNVLRGASTALILSFGTALVAMPLGVLLGILA
                     GWSGGKLDDLIQWLMSTVASIPWLLFVIAFLMVFGRGLFWICLAIGLTSWVGTARLIR
                     GETLKQKSLDYITAARATGLSVPRILFRHLLPNVRYLIIIDLTLFCSSVILAESILTF
                     IGIGVEQGTASWGAMLIESEKELMRSPAIWWVFAGSAFCGILPLVLSLNVLGDALRDA
                     LDPRAVSSR"
     gene            complement(1205..2188)
                     /locus_tag="PKK76_02445"
     CDS             complement(1205..2188)
                     /locus_tag="PKK76_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNL67350.1"
                     /translation="MLTYIIRRIIYTLPIWIGVYGITFALFHMRDPIAIARVHMPQAP
                     DAALKAWVRNNNLHLPRFVNLPADADTVLVDGKKHPELASKSIFYSQFFLGFREIITF
                     DFGVDKNRQPISESIAERAPYSLSVMLPAFLITVTLSVVMSLFLAYFRETGLDVGAVL
                     ITVAMMSIPVPVYLLLCNWGFGKYLKLVPVYNHVLLPILVSVLAGLGGQVRFYRTVFL
                     EQIEQDYIRTARAKGVSESGILFKHVLPNSLIPILTSVVMSLPFLITGSLILEHFFGI
                     PGMGDMMYTAIYSQDFQVIKVMVYLGAFLYMIGALLTDISYILADPRVVLE"
     gene            complement(2188..4131)
                     /locus_tag="PKK76_02450"
     CDS             complement(2188..4131)
                     /locus_tag="PKK76_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNL67351.1"
                     /translation="MRAGVIFFALFLAACNNNPYGQFPPGKVIMSNLPDDPRTLDPAR
                     VGDTQSNAIASNLHDTPFEYYYLKRPLEIKPAMAVSMPRTGAAVSGGTQYRTFEFSIK
                     KGMRYADDSCFAGGKGREIKIDDIIFAVKRAADNSIDPFGLPLLSGKVLGFDEYTTRL
                     EKAHAEEAEKRDSSIVSKVFDSEIPGVRKLDDYTLQLLLTEDYPQIIFFFTLTTGSPQ
                     PRECVEYYNGRNGRPAYDRHPATSGAFRLKEWHSNYRIVLARNPNYRSDDFYPREGTE
                     EDKANGLLLAAGRQLPIVDEIWIQIIKAGPPVWTLFDQGYLDRAGIPLEVFNQVVLNQ
                     ELTPEFKERGTRLDTEIDQATYYWVFNLTDPVMKNKKLRQALSLMLDRREMLERFFNG
                     RGVPAMSIIPPGMEGYEDEYKNPYAAYDPDRARVLLAEAGYPGGIDPATGRALRVSFT
                     LTNSPGSTSLYAFYRSSFEKFNVDLKLEEADWPTVLQKKYKKEFQIIVGGWHADYPDP
                     QNFLQLLYGPNTASSYNEGSYRNPTYDSLYLQMKNMPPGPARLAIIRKMKEIVADDTP
                     VIMQFHPITYGLSHPWFSPFKTHPTNSNQLKFRDLDPEKRKTLVAQWNRMPLAGYLLF
                     AFLILVLVLPVVIAVRKYSRRLR"
     gene            4238..4705
                     /locus_tag="PKK76_02455"
     CDS             4238..4705
                     /locus_tag="PKK76_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNL67352.1"
                     /translation="MKRAILIFLLVSALKAQTTDVADRKVTLYDGQGITELAQKYLGV
                     PYRFGGQSPDGFDCSGLTGYVYKKAGYQLPRDAGDQFKALRPIKTPRPGDLVFFKIDG
                     KSIGHVGIYLGNLRFIHAPSTGKTVSIADMRISYWKTRYAGARTIFGQAEAAR"
     gene            4724..5194
                     /locus_tag="PKK76_02460"
     CDS             4724..5194
                     /locus_tag="PKK76_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNL67353.1"
                     /translation="MSAHSCSVITKRIQEAGLILPQVNPPIASYVPAVRSGSTIFTSG
                     QLPMKEGQLLMTGIMTASRSLEEAQSAMKQCFLNAIAAALLVAREDEIKGVLKLGAFV
                     ASAPDFTDQHKVANGASDLAKLIFGDAGVHARFAVGVASLPVNATVELEVIFSV"
     gene            5206..5823
                     /locus_tag="PKK76_02465"
     CDS             5206..5823
                     /locus_tag="PKK76_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67354.1"
                     /translation="MIQTRNIVTVLKQAAEFEKDFARASFVGALQMARHVALEIKHTV
                     TNLSVFHLSYYLASCVLILVALAPWIEYQIDFGTPESVDVGSSAKLIFLLPGLVGLLV
                     ATFELPFRVQAYRISAGLAVLVWIGGLIFPNPVHTSIHAGEFHLRWWLYLYGPLLLAQ
                     GTLSLQGLTEASIHLDRFQDRLFPPPAAPEGKAPSRRKGAKPGRA"
     gene            complement(5804..6022)
                     /locus_tag="PKK76_02470"
     CDS             complement(5804..6022)
                     /locus_tag="PKK76_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67355.1"
                     /translation="MIVSKLESPLYHRQKREGLPGRGYEAPVAANRKSFDQYLLEAFQ
                     GEVVQEGKRFSSGISPLQRENLIKLGRV"
     gene            6291..7481
                     /locus_tag="PKK76_02475"
     CDS             6291..7481
                     /locus_tag="PKK76_02475"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNL67356.1"
                     /translation="MKRIVILCLFGPAWILARDLEKRPGRFVPRAEVVSVQPEKNRLC
                     AETVEIPSLKGKDASSVRSFFFEKEKNMILLTEDGSEFGTLTIDRVEPGKPHLICGEF
                     ALNAEQSPRPGDLAGVTLQPANYRTQNLRGPLPVSPPERIRNQADKKMMVRVEWDYVV
                     FGQGEDPAMDNFNPHFFDRDPSVTPKVETFYMDEYEVTNREYLFFTRQTGHRLPESWE
                     KTGSYPEGMEDHPFILASYEDAAAYARWSGKRLPTEIEWEMAARGGLKLLRNGTGVNG
                     ITRSPRVFPMGEWKSEVCNTREMWQDEPRTVSVYTLKDRSPYGIVGMCGSAAEWTETW
                     YNPYPGASKFPMNLAGKQFRVIRGGSFHQDRDAARADSRDYGGFESPENQKRAGIRLV
                     ISAK"
     gene            7628..7810
                     /locus_tag="PKK76_02480"
     CDS             7628..7810
                     /locus_tag="PKK76_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67357.1"
                     /translation="MKTIAIALFAVFAASCGLIDRKIAGYTGYSKICIDGVRYIQFTS
                     GASVKYTKDGRVETCD"
     gene            complement(7824..8750)
                     /locus_tag="PKK76_02485"
     CDS             complement(7824..8750)
                     /locus_tag="PKK76_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL67358.1"
                     /translation="MTHERSTIVIVDDDPLILEVLQRGLASQPDMSVLIFPNGESYLE
                     FFASTKPDLLLLDAGLPGISGLEVLARIRARYSRTELPVIFISSDSESAQVVSVLEAG
                     ANDFVGKPVDLPVLIARMKVHLSLPGSRSPTPSQPSSTFVEGQTYPLAFLYCQVVVDQ
                     KFSVSRQGSEIQSSLGVLFDMYSRTAQRYNGQLWLRKDDAGFFVFHKQDAVSVVMCAI
                     ELMTLNVLHGALQDRSRDFGINIGLDLGDTVYRADPSILQSAALNKSAHLAKEDGRGR
                     LRISQSIWAQLSPAARRYFDEGPDCWIYRSIF"
     gene            complement(8747..9634)
                     /locus_tag="PKK76_02490"
     CDS             complement(8747..9634)
                     /locus_tag="PKK76_02490"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL67359.1"
                     /translation="MFRSSVLQAIGHSLTTDQMDILAHEVNESFNLGVVSGFGSSIRV
                     PLQVAVRCIGDFFAQDKDLLAFLAHVLAREGKFVAGSQVLLKGKAALLELLEAESWHY
                     DREMRQFVRDQAMERTADWGHMAEGREYHLTFASVDVVGSSQFGHENAQVFAQMREYV
                     RNYAEVWNGRLWNWMGDGGMAAFHGADSVNRCVISMLAVLMNLPVFNIKYLAQGPELR
                     IRVGIHYGTATYKQPVNEIFSSDMRVAQKLESEVSEPNRMAISGSVYTFLKPEMYPCF
                     VAGPRFDHLETYRLTEIPL"
     gene            complement(9669..>11238)
                     /locus_tag="PKK76_02495"
     CDS             complement(9669..>11238)
                     /locus_tag="PKK76_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL67360.1"
                     /translation="IALLFAFSVLFLTPLHFTRFIDYYYIVVGLASVFLLFGIARGLF
                     KKVQGVGPVGLGLLLMVAAYANDVLYNKNIINTALLSPYGIIVFMFVQAVGLSKRFSR
                     AFALSESLSENLTRTNAELVSWKNRLEERVAERTREIETARSIAENSSQAKAEFLAHM
                     SHEIRTPMNGIMGMLRLLDDAKLGEDEQRYLSTIRASSETLLTILNDILDISKIESGA
                     MTLTDEPVVIKDLITSLTAMTEPAAQRKGLQLLVIFPLNMPQAILTDRTRVYQILLNL
                     LSNAVKFTDSGTITLKVDRDNGMIRFQVKDTGIGIPPDKFGLLFQSFTQLDSSPARRV
                     GGTGLGLAISLQLAHLMGGEITAESIPGGGSSFILILPERPTTVDTTRVDTPAAPSDL
                     LPEGLQVLAAEDNEINRIVFQKIGERLRMTVDTAEDGLTAVDMSLLKRYDIIFMDIEM
                     PGLDGVGALKKIQEKLGESAPPIVAVTANAMAGVREKLLTAGFKAYISKPYSPEDIVD
                     AVLAVIRPSSVQKS"
BASE COUNT         2973 a         2924 c         2780 g         2561 t
ORIGIN      
        1 atgctgacca gcctgacaga acaaaaaaga gcaacacgcg aaatcgaaag cacaattgga
       61 aacgtggcaa ctacggcaca aacagtcagc tcaggcactc atgaacttcg cactctcgcc
      121 acgcaacttc gtgaaaaatc agaagaactc ctgggtactg cttcgcgctt ccagatctaa
      181 cgcgaactca cagcgcgagg atccagtgcg tcgcgaagcg catccccgag cacgtttaga
      241 gacaggacca gaggaaggat tccgcaaaaa gcgctcccgg caaaaaccca ccagatggcc
      301 ggagatcgca taagctcctt ttccgattcg atgagcatag cgccccagga ggccgtgccc
      361 tgctccactc caatcccgat gaaagtcagg atgctctctg caagaatcac ggacgaacag
      421 aacagagtca gatcaatgat gatcagatat cgcacgttcg ggagcaaatg gcgaaagagg
      481 attctgggaa cggaaagacc ggttgctctg gctgctgtga tgtagtccag actcttctgc
      541 ttgagagttt ccccgcgaat caatcgggcc gttcccaccc acgaagtcag gccaatcgca
      601 agacaaatcc aaaaaagtcc gcgaccaaac accatgagaa atgcaatcac aaagagaagc
      661 cagggaatag acgccaccgt agacatgagc cattgaatca gatcgtctaa ctttccgcct
      721 gaccaaccag caagaattcc cagaagcaca ccgagcggca tggcaacaag agctgtccca
      781 aaagacagaa tcagcgccgt gcttgcaccg cgaagaacgt tgtgcattac gtccacaccg
      841 ttgatgtccg ttcctagaat gtgaatgcct ttgactcgat tgagctccct gtctgcctcg
      901 agcatgcgaa tgccggtcac ctcggatgcg aaaggcgcgc tgtatgtgcg ctctttctga
      961 atccactgca aacttaaatc cagcaggctg cgatccgcga ttctctcttt ctctggaact
     1021 ttgatttgcc caatcaagcc aacaccaagg taagcgcata ctacgtagaa ggcgatgcgc
     1081 actcgcaagc ggctagcgag catgtcacgc gcatccttca aagaaggatt tcgcaagaaa
     1141 ctcctcaatg ccacgatgcc tgccgctgcc gcgagaatcc aggcaatgtc gatcagaatc
     1201 gtgatcattc gagcaccacc ctggggtctg ccagaatata ggaaatgtcc gtgaggagtg
     1261 cgccaatcat gtagaggaac gcacccagat agaccatcac tttgatgacc tggaaatcct
     1321 gactgtagat ggcagtatac atcatgtcac ccattccagg aatgccaaag aaatgttcga
     1381 gaatcagtga gccagtgatc aagaaaggca aagacatgac cacagaagtc agaatgggaa
     1441 tcaaagaatt tggtaataca tgcttgaaca ggatgccgct ctcgcttacg ccctttgctc
     1501 tggcggttcg aatgtagtcc tgctcgattt gctccagaaa gacggtgcga taaaagcgca
     1561 cctggccacc cagtcctgca agcaccgaga ctaagattgg aagcaacacg tgattgtaca
     1621 cgggaaccag ttttaagtac ttgccaaagc cccaattgca gagaagcaga tagacgggca
     1681 caggaatgct catcatcgca acagtaatga gaacggcgcc gacgtccaga cctgtctctc
     1741 tgaagtacgc aagaaacaag gacatcacta cgctgagtgt gactgtaatc aagaatgcgg
     1801 gcaacatcac tgacagagaa taaggagcgc gctctgcaat cgactcactg attggctggc
     1861 gattcttatc cactccaaaa tcaaacgtga tgatctcacg aaagccaaga aagaactgcg
     1921 aataaaaaat gctcttagaa gcgagctctg gatgtttttt gccgtccacc agcactgtgt
     1981 ccgcatctgc aggaagattc acaaaccttg gtaaatgcag attgttgttg cgaacccagg
     2041 ctttgagagc ggcgtcgggc gcctgcggca tgtggactct cgcaatcgca atgggatcac
     2101 gcatatgaaa caacgcgaag gtgattccgt atactccaat ccagatggga agggtataga
     2161 tgatgcggcg aatgatatac gtgagcatca gcgaagtctc ctcgaatact ttcgcacggc
     2221 gatcacaacg ggcaacacaa gaaccagaat gagaaatgcg aacagcaaat atccggccag
     2281 cggcatccgg ttccactgcg ctacaagagt cttgcgcttc tctggatcaa gatcgcgaaa
     2341 tttcaattga tttgaattcg taggatgcgt tttgaacgga gaaaaccacg gatgagacaa
     2401 cccataagtg atgggatgaa attgcatgat tacaggcgtg tcgtctgcga caatctcctt
     2461 cattttacga atgatggcca ggcgagccgg tccaggtggc atgttcttca tttgcaaata
     2521 gagagaatca taagtcggat ttctgtagct tccctcattg tatgaactcg ctgtgttcgg
     2581 gccatagagc aattgtagaa aattctgcgg atcaggatag tccgcgtgcc agccaccgac
     2641 tatgatctgg aattccttct tgtacttttt ttgcaaaaca gtgggccaat ccgcttcctc
     2701 caatttcaga tccacgttga atttctcaaa actcgatctg tagaaagcat agagactcgt
     2761 tgagcctggc gaattcgtaa gagtaaacga aacgcgaaga gcgcgaccgg tggccggatc
     2821 gataccaccc gggtatcctg cctccgccag aagcactctc gctctgtccg gatcgtaagc
     2881 cgcatacgga ttcttgtatt cgtcttcgta tccctccatt cctgggggaa tgatgctcat
     2941 ggccggaact cctctgccgt tgaaaaatct ctccagcatt tcgcgccgat cgagcatgag
     3001 gctgagcgcc tgacgtagtt ttttattctt catcacggga tctgtaagat tgaaaaccca
     3061 gtagtaagta gcctgatcaa tctccgtatc cagacgagtt cccctttcct tgaactcagg
     3121 agtgagttcc tgattgagaa caacctgatt gaacacttca agcggaatcc cggcccgatc
     3181 caggtaacct tgatcgaata acgtccagac tggaggaccg gctttgataa tctgaatcca
     3241 gatctcatct acaatcggaa gctgacgccc ggccgcaagc aaaaggccgt tcgccttatc
     3301 ctcttctgtt ccctcacgtg gataaaaatc gtcagagcgg tagttggggt ttcgtgccag
     3361 cacaatgcga taattggaat gccattcctt caaacgaaaa gcgcctgacg ttgccggatg
     3421 acggtcatac gcgggtcttc cgtttcttcc gttgtaatac tcaacgcatt cgcgaggctg
     3481 agggctgcct gtcgtaagag taaaaaagaa aatgatctgc ggatagtctt ctgtcaacaa
     3541 gagctggaga gtataatcgt ccagctttcg cacgcctgga atttctgaat caaaaacttt
     3601 tgaaacgatc gagctatctc tcttttctgc ttcttctgcg tgcgcctttt cgagacgcgt
     3661 ggtgtactca tcgaatccaa gtactttgcc ggaaagaagc ggaagaccaa atggatcaat
     3721 cgaattgtcg gccgctcttt tgacagcaaa gatgatatcg tctattttga tttcgcgccc
     3781 ctttcctccc gcaaagcaag agtcatccgc gtacctcatc cctttcttga tcgaaaattc
     3841 aaacgtgcga tactgcgttc ctccagaaac tgccgccccg gtcctgggca tcgatactgc
     3901 catcgcaggc ttgatttcaa gaggtcgctt caggtagtag tattcgaaag gagtgtcgtg
     3961 tagattcgaa gcgattgcat tggattgtgt atcgcctacg cgcgctggat ccagcgttcg
     4021 cggatcatcc ggcaaattgc tcatgatcac tttaccggga ggaaactggc cgtacgggtt
     4081 gttgttgcat gcggccaaaa aaagtgcgaa aaaaattacg cctgcgcgca ttacccgata
     4141 tcaggccaca gaaaatgctg gcaactgctt atgcaggatt gcgtttctaa agggggttcg
     4201 aactggattc gatcccattt cgtccgatac taagaatatg aagcgagcga ttctgatctt
     4261 ccttctcgtg tctgccctca aggcccagac cacggacgtg gccgatcgca aggtcactct
     4321 gtacgacggc cagggaatca cagaacttgc gcaaaagtat ctgggtgtac catatcgctt
     4381 tgggggacaa tcgccggatg gctttgattg ttccggtctg accggatacg tttataaaaa
     4441 ggcgggctac cagctcccca gggatgcagg agatcaattc aaagcgcttc gccccatcaa
     4501 aactcctcgt ccgggagacc tcgtgttctt caagatagac ggcaagtcga tcgggcacgt
     4561 tggaatctat ctgggcaatc tacgattcat ccatgcaccc tccactggaa agaccgtgag
     4621 catcgcagac atgaggatct catactggaa aaccaggtac gcaggtgctc gcacgatctt
     4681 tggtcaggcg gaagctgcga gataaattac ttttcgcgag gctgtgtctg cgcattcttg
     4741 ctctgtgatc acaaagcgca tccaggaagc agggctgatc cttccgcagg tgaacccacc
     4801 catcgcatcg tatgtcccgg ccgtcagatc cgggtccact atctttacca gcggccagct
     4861 ccctatgaaa gagggacagc ttttgatgac tggcatcatg actgcatcgc gcagtctaga
     4921 agaagcacaa agtgcaatga agcaatgctt tctcaatgca atcgcggccg cacttctagt
     4981 cgccagggaa gatgaaatca aaggtgtgct caagctgggc gcttttgtgg caagcgcgcc
     5041 agattttaca gatcagcaca aagtagcaaa cggtgcgtcg gatcttgcaa agctcatctt
     5101 tggagatgca ggcgttcatg cccgattcgc agtgggagtt gcttccctcc cggtaaacgc
     5161 caccgtggaa ctggaagtga tcttttccgt ctgagcgggg agctgatgat tcagacaaga
     5221 aacatcgtca cagtcctcaa acaggctgcg gagtttgaaa aagacttcgc tcgcgcttcc
     5281 ttcgtagggg cgttacaaat ggcgcggcat gttgctttag aaatcaaaca cacagtgaca
     5341 aatctgagcg tgtttcatct gagctactac cttgcgtcct gcgttctgat tctagtggct
     5401 ctggcaccgt ggatcgagta tcagatcgat tttgggacgc cagaatctgt agacgttggt
     5461 tcatctgcaa aactcatatt tctcttgcct ggtctggttg gattgttggt ggccaccttt
     5521 gagctgccat tccgggtgca ggcctacaga atcagtgcgg ggctcgcagt tctggtctgg
     5581 attggcgggc tcattttccc gaatccagtc cacacaagca ttcatgcagg cgaatttcac
     5641 ttgagatggt ggttgtattt gtacgggccg cttctcctgg cccagggaac tctctcactg
     5701 caagggctga cagaggcctc catccatctt gatcgatttc aagacaggtt gttcccgccg
     5761 cccgccgcgc cggaggggaa ggccccctcc cgccgcaaag gcgctaaacc cggccgagct
     5821 tgatcaggtt ctccctctgg agaggactga tcccggaaga aaaccttttg ccttcctgga
     5881 ccacttcccc ctgaaacgct tctagcaggt actgatcgaa actcttcctg tttgctgcaa
     5941 caggagcctc atagccacgt ccgggaagcc cttcccgctt ttgacgatgg tagaggggac
     6001 tctcgagctt gcttacgatc atactggcct ccacacacat cctttgacgg ccttttcccg
     6061 acaaagttta tgtctcatac aaaatttact acccacgagc ctaaaataca agagtttttt
     6121 gcccgacaac actatacaaa gaatacctaa acaaaagtca agcattcttg tgttgggcgc
     6181 ctcttttttt cttctcgctt gtgtatcttt ttacccgccc cctgggacaa aaacctcccc
     6241 cctttgcaaa aaacccaaaa aattggaact ggcgggccga ttctaagaaa atgaagcgaa
     6301 tcgtcattct gtgcctgttt ggtcccgcct ggatcctggc ccgagacctg gaaaagcgcc
     6361 ccgggcgatt cgtacccaga gcagaagtgg tttccgtgca gcctgagaaa aacagactct
     6421 gtgcggagac cgtggagatc ccatctctca agggcaaaga cgcctcttct gtcaggtcct
     6481 tcttttttga aaaagaaaag aacatgattc tgctcacaga agatggatcc gaattcggca
     6541 ccctcaccat tgaccgcgtg gaaccaggga agcctcacct catctgcggc gaattcgcat
     6601 taaacgcgga acaatcgccc agaccgggag acctcgccgg tgtaacactg caacctgcta
     6661 actacagaac tcaaaatttg cgcggtccct tgcccgtttc gcctcccgag agaatcagaa
     6721 accaggcgga caagaagatg atggttcgag tggaatggga ctacgttgtg tttggccagg
     6781 gcgaagatcc tgccatggac aatttcaacc cacacttctt tgatcgcgat ccttccgtga
     6841 cgccaaaagt ggaaaccttc tatatggatg aatatgaagt aacgaatcgc gagtatcttt
     6901 tcttcacacg tcaaacagga cacagattgc cggagtcctg ggaaaaaacg ggatcgtatc
     6961 ctgaaggaat ggaggaccat cctttcatac tggcgagtta cgaagatgca gctgcttacg
     7021 cgcgatggag tggaaagaga ttgcccacag aaattgaatg ggaaatggca gcgcgcggtg
     7081 gactaaaact gcttcgaaac ggcactggag taaatggaat cacgcggagc ccgcgcgtat
     7141 tccccatggg agaatggaaa agcgaagtct gcaatacaag agagatgtgg caggatgaac
     7201 cgcgcactgt atcggtgtat acactgaaag acagaagtcc ctacggaatc gtgggaatgt
     7261 gtggcagtgc tgctgagtgg acagagacgt ggtacaatcc atatccaggt gctagcaaat
     7321 ttccaatgaa tctagcgggt aagcaattca gagtcattcg cggtggttcg tttcatcaag
     7381 acagagatgc agcccgcgcg gattctcgcg actacggcgg attcgaatct cctgaaaacc
     7441 aaaagagagc ggggattcgg ctcgttatct ctgcaaaata gtcgagtatc attcaataag
     7501 gaattgtgcg cgccggcatt cgcagcggat cgcctcatcc atgtttggcc cggcaaatct
     7561 ttcgctcgcc tatgcaaggg gcagtataat cgcttgcgcg gcgaatagtt gtttgaaaag
     7621 agttctcatg aaaacaattg caattgctct tttcgcagtc ttcgccgcaa gctgcggcct
     7681 cattgaccgc aagatcgccg gatatactgg atactcaaaa atctgtattg acggagttcg
     7741 ttacattcaa ttcacttcag gtgcttccgt gaaatatacg aaagacggcc gcgtagaaac
     7801 gtgtgattga ttgaggcgaa tgcctagaaa atggatctgt agatccagca atcgggtcct
     7861 tcatcgaagt aacggcgcgc tgctggactg agctgcgccc agattgactg ggaaattcta
     7921 aggcggccac gtccgtcttc ttttgcaaga tgagcgctct tattgagcgc cgcactctgc
     7981 aggatagagg gatctgctct gtaaacagtg tctcctagat ccagcccgat gttgattcca
     8041 aaatcgcggc tgcgatcctg caaagcgcca tgcagtacgt tcaatgtcat cagctctata
     8101 gcacacatga ctacagaaac tgcatcctgc ttgtgaaaca caaagaagcc agcgtcgtct
     8161 ttgcgaagcc agagctgccc attgtagcgc tgagcggttc tcgaatacat atcgaagagc
     8221 acgccaagac ttgactgaat ctcagaaccc tgcctggaaa cggaaaactt ctgatccaca
     8281 accacctggc aatagagaaa tgcgagcgga taagtctgac cttccacgaa cgtagaggag
     8341 ggctgagacg gagtgggact ccttgagcca ggaagagaaa gatgaacctt cattctggca
     8401 atgaggacag gaagatcgac aggcttgccg acgaagtcgt ttgcacccgc ttcaagcaca
     8461 gacacgactt gcgcggattc cgaatctgat gaaataaaga tcacaggaag ctctgtgcgc
     8521 gaataacggg cgcgaatgcg agcaagcact tccaggcctg agatacctgg caacccggcg
     8581 tcgagcagca gcaaatcagg cttggtggag gcgaagaact caagatagct ttccccgtta
     8641 ggaaaaatca aaaccgacat gtctggttgc gaagcaaggc ctcgctgaag aacttccagg
     8701 atcagaggat catcgtccac gatcacaatg gtagagcgtt cgtgggtcaa agtgggatct
     8761 ccgtcaggcg atacgtttca agatgatcaa agcgtggccc cgctacaaaa cacggataca
     8821 tttccggctt caaaaaagtg tacacagatc ccgaaatcgc catgcgattt ggttcgctga
     8881 cctcggattc cagcttttga gccacacgca tgtcactgga gaagatctcg ttgactggtt
     8941 gtttgtacgt tgctgttccg tagtgaatgc caacgcgaat ccgaagttcc gggccctgcg
     9001 cgaggtattt gatattgaag actggtagat tcatgagaac agccagcatg gaaatcacac
     9061 atcgattcac tgaatccgca ccgtgaaaag cggccatgcc tccgtctccc atccagttcc
     9121 agagacgacc gttccacacc tccgcatagt ttcgcacgta ctctctcatc tgtgcaaata
     9181 cctgcgcgtt ctcatgaccg aactgcgaac tgcccactac atctaccgaa gcaaaagtca
     9241 gatggtactc acggccctct gccatgtgtc cccagtccgc agtgcgctcc attgcctggt
     9301 ctcgcacgaa ctggcgcatc tctctgtcgt agtgccagct ctctgcttcg agcaattcga
     9361 ggagagctgc ttttcctttt agtaatactt gagaacctgc aacaaatttc ccttcgcgtg
     9421 cgagtacatg agcaaggaat gcgagcaaat ccttgtcctg cgcaaagaaa tccccgatac
     9481 aacgcacggc gacctgaagg ggtacacgaa tgctcgatcc aaaaccggaa accactccca
     9541 ggttgaacga ttcattcacc tcatgggcga gtatgtccat ctgatctgta gtaagagaat
     9601 gacctatggc ctgtaatacg ctggaacgaa acacgagaga cagaataaaa aagcgcccgc
     9661 aattgcagtc aacttttttg aacagaagaa ggccgaatta cagcaagaac tgcatctaca
     9721 atgtcttctg gcgaataggg cttgctaatg tatgccttga aaccggcagt gagcagcttc
     9781 tctcgaaccc cggccattgc attggctgtg accgccacaa ttggcggggc agattctccc
     9841 agtttttcct ggattttttt caatgcacca acaccatcga gaccaggcat ctcaatatcc
     9901 atgaagatga tatcgtagcg cttcaagagg ctcatatcca ctgcagtcaa gccgtcctct
     9961 gctgtatcca cagtcatgcg cagacgctct ccaatcttct gaaacacaat ccgattgatt
    10021 tcgttgtcct ccgcagccag cacctgaaga ccttccggaa gcagatcgga aggagcagcg
    10081 ggagtgtcca ctctagtagt gtccacggtc gttggtcttt caggcagaat cagtatgaat
    10141 gagctacccc cgcctggaat ggattctgct gtgatttctc ctcccatgag gtgagccagt
    10201 tgaaggctga tcgcaaggcc aagccccgtt ccacctactc ggcgcgcagg cgaagagtcg
    10261 agttgcgtaa atgattggaa aagcaaaccg aatttgtccg gcggtattcc aatgccagtg
    10321 tctttcacct gaaagcgaat catcccgttg tcgcggtcta ccttcagagt gattgtgccg
    10381 gaatcggtga atttgacagc attggaaaga aggttcaata gtatttgata cacgcgggtg
    10441 cgatcggtca aaatcgcctg cggcatgttt aacggaaaaa tcacaagcaa ctgcaaacct
    10501 ttcctctgcg ctgcaggctc cgtcatggca gtgaggctcg tgatcaaatc ctttatcaca
    10561 accggctcgt ccgtaagagt catcgcacca ctttcaattt tggaaatgtc cagaatgtca
    10621 ttgagaatag tcagaagcgt ttcagaagaa gcgcgaatag tagaaaggta tctctgttcg
    10681 tcttccccga gcttcgcatc atccaacagc cgaagcatac ccataattcc attcatagga
    10741 gtgcgaatct cgtgactcat gtgagccagg aactcggctt tcgcctgcga agaattttcc
    10801 gcaatggaac gagctgtctc aatctctctg gttctttcag ccactctctc ttcgagcctg
    10861 tttttccagc tcacaagttc tgcattggtt cgcgtcaaat tctctgaaag ggattcagac
    10921 aacgcgaaag cgcgcgagaa tctcttgctg agccccaccg cctgcacaaa catgaataca
    10981 atgattccat agggagaaag aagagctgtg ttgatgatgt ttttgttata aagaacatca
    11041 tttgcgtatg ctgccaccat aagcagcaga cccagaccta cggggcccac accctgcact
    11101 ttcttaaaca gtccgcgcgc aattccaaac aataaaaaaa cagaagcaag cccgaccaca
    11161 atgtagtaat agtcaatgaa cctggtgaaa tgaagcggag taagaaacaa aacagagaac
    11221 gcaaataaga gtgcaatg
//
LOCUS       DAORFF010000033        11197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2610_length_11197_cov_3.997487, whole genome shotgun sequence.
ACCESSION   DAORFF010000033 DAORFF010000000
VERSION     DAORFF010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2610_length_11197_cov_3.997487"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PKK76_02500"
     CDS             <1..1335
                     /locus_tag="PKK76_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNL67361.1"
                     /translation="PRVSGFTRDRLPSPITSVQSRLPMRNIAVLSDDADLNAKIASSC
                     NSFGDEFQAVFFHEERAFVQYLNYELPEIDIIDFSGSKASAGRAFTEIKDDPWLHFGG
                     SIIIYEKQKEEDLLNRLRGVNIIAHIQKSKVELYLPRALNVLSQHRSILFQRDLHALL
                     QSNISGMFVLDNDPFDVTTYSNLIANFLYNSNLINLDQKSNCSAALVELLYNAIEHGN
                     CCIGFEEKKVWLENGGDILDLIRQKAMKPEILAKKIFLNYRITPERSYFTVRDEGEGF
                     DWGSRLAEDNPAEALHGRGIMIARNYLQNVHYNEKGNEVSFQIEHLHESNVIPRVFAD
                     QEEVTVQDGEVIFRQGETSSHLYYIISGKYDIIANGRKVSTLTPADIFLGEMSFLLNN
                     KRSATVIAVGRGQLMKISKQAFINAIREHPHYGIFLARLLAQRLDQVHEVGL"
     gene            1351..2670
                     /locus_tag="PKK76_02505"
     CDS             1351..2670
                     /locus_tag="PKK76_02505"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL67362.1"
                     /translation="MIRTVLRGFRRVSAVILGRPDFANLDQRLFNVMCFGGTIVAVLS
                     AIENAFIDVPFVLVVGSCVQTVILASGFVVSRYWNIYRPIVWPVILAILVFLAMQWFW
                     NAGSVGGAHYFFFVCGMMGAVIMRGWQRYFVILLYLVVINGVLYLEYIRPDLIVNYSD
                     RQSRYVDIAFSLSLCMILFGLVIAIFSGHYQVLVNRVQRHKLEFVEDLHLARILQETV
                     FQHDRTLTQGFDCEIVYRPSAALGGDLYDLSRRLGILRVFMADMKGHGVNAALSSMLI
                     KSEWVHSGHASFSAGDALANLNKQVVARYGDSLVLSAIVADLYEDSIHFAAAGHVPQF
                     RVFGGEVLRLEATGVPIGVMEDSQYSTERTALPRGSRLVFFTDGFHDQPDLSGKTIGT
                     DWMEEILRKSQPSSADMVRALISKFHAKTGTEPGTTADDLTLIVIGR"
     gene            complement(2674..3363)
                     /locus_tag="PKK76_02510"
     CDS             complement(2674..3363)
                     /locus_tag="PKK76_02510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNL67363.1"
                     /translation="MNPVDFQGLRLLRKKGRKRLTVFVLADNVIEVRAPLRTSDRVVR
                     EFLLQAAPWIRQKLQANQERPSAARLRYVHGEKIPWQGKEILLHVLPGIRQSRLTDES
                     LTVHARNALIAKKKVAAVYSKHGEKIFRETVSRFAPIVGKHPSRISVRGMKSRWGSCS
                     HTGSLSLNWKILAAPQYVIDYLIVHELCHLIHANHSSRFWAEVERVLPEYKEGRKFLK
                     DHGSSILIRFD"
     gene            3472..4173
                     /gene="ric"
                     /locus_tag="PKK76_02515"
     CDS             3472..4173
                     /gene="ric"
                     /locus_tag="PKK76_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNL67364.1"
                     /translation="MILSSDTLSKIALSAPHATRVFAKYRMDFCCGGGTSLSDACIGK
                     GLNSESVLAEIQAAAPLRRSVEADAPLGEVVDHVLSTHHEFTRNIMAEIEKFSVRVAA
                     VHGAQHPEMIVLRDLFADFKAEMELHLLKEERVLFPYIRLLENGAEKDAPVCFSDVGG
                     PIRMMLFEHENAGEDLRKMREVTSDYTLPQAACNTYRLLFHKLQELEEDVHLHMHLEN
                     NLLFPRAMELAANPG"
     gene            4225..5220
                     /locus_tag="PKK76_02520"
     CDS             4225..5220
                     /locus_tag="PKK76_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HNL67365.1"
                     /translation="MLRFLTIACVALCSCQWLIRPGTDFEKSPPPPEPDYSLRESWAA
                     LPDRADTADSVPSEADWKDAQSTASADVFFVHPTLYFGGTWNADVADATVNRRVDETT
                     LRKQASVFNCCARIYAPRYRQATIAAFLDKKNGAPALDLAYSDVLRAFDYYMIHYNQG
                     RPVIIASHSQGTRHAYFLLKDRFDRKPALSRLVAAYLIGMAIPEHPYATIPICKSATE
                     VRCFITYRSATPGTELKRLPHDPEPPYACTNPVHWKSDDSLAEADLNPGGVSANFEKV
                     DPNVCSAQCQNGVLLISKPKESGYHNISGNFHVSDYSLFYVSIRKNVADRIAAFR"
     gene            5217..5963
                     /locus_tag="PKK76_02525"
     CDS             5217..5963
                     /locus_tag="PKK76_02525"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNL67366.1"
                     /translation="MSVLVLVLVVLFCSIALLFPRKQARSSSRIAAVKRNSPVILRQL
                     VYRGGDWDANPGSLRNLARFASNCEAIKLDVRAGEPANGRDLPGSDFFVYATGHHGFD
                     LAGSDLTGFLSFVRAGGFVLVDDNRGMDAAFRRFVAQNFPEARLEPVRSGEEVFRKPY
                     NVGSMPKIMIHDGSPAVLYRLKEGQDLNLYYSFSSDLGDGWEDSLRHRVPEETRRLAL
                     EMGTNLIALAFAWSSAARERRGNFAARNSS"
     gene            complement(6001..6159)
                     /locus_tag="PKK76_02530"
     CDS             complement(6001..6159)
                     /locus_tag="PKK76_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HNL67367.1"
                     /translation="MDIVRIIAAIVLPPLGVFLQVGIGFHFWLNILLTIFGYVPGIIH
                     AIWVILKK"
     gene            complement(6170..6583)
                     /locus_tag="PKK76_02535"
     CDS             complement(6170..6583)
                     /locus_tag="PKK76_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67368.1"
                     /translation="MKPLLLTMALFFSSHALFAQEAILSKPKACLAVDENAGTPSGCK
                     TKFRAADKIHVFSNIETPGVYKLNAVFELQDDTRKNKPINIDIDTQADWNYVYFWMTP
                     NTHDDKYLGTWKITIYVHTEDGDTSEYSTTFQVVK"
     gene            complement(6628..7272)
                     /locus_tag="PKK76_02540"
     CDS             complement(6628..7272)
                     /locus_tag="PKK76_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNL67369.1"
                     /translation="MDRRKFFREGVKEVAREVMKTPVGSAIDKQLLAFSNLLSPEHFT
                     PPAPVPPADAPPPRKLARPPGALKDPKKFNKACTKCGDCIVACPYGVLFQMGPASGPL
                     LDPNLNACRLCPDMPCIDSCETGALKKLRKKQNPRLGVAAVSESLCRNHESRKPRLAP
                     GQKSQTCKICVRECPVKGAISLGKDRLPDIAKSCTGCGQCVHVCPNHAIRVDVF"
     gene            complement(7259..7660)
                     /locus_tag="PKK76_02545"
     CDS             complement(7259..7660)
                     /locus_tag="PKK76_02545"
                     /inference="COORDINATES: protein motif:HMM:NF039485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HNL67370.1"
                     /translation="MGFLDKVISAANDVAGKEGNQNLVGGVIDMLQKKGVGGIVQDLK
                     TKGLGDLAESWIGTGENKLISPEQIKNVLGSEQLQALAQKAGVSPENASKFLGQILPG
                     IVDKLTPQGQVPGDDPGKSPEASAGPSDGST"
     gene            7731..8729
                     /locus_tag="PKK76_02550"
     CDS             7731..8729
                     /locus_tag="PKK76_02550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL67371.1"
                     /translation="MNPEILRRYYMQVLEAPKKKRRAPKKSKDPFHPPLFEARPGHVR
                     LVPCNACGGMSFKTLFEKESSLKEVFQVVRCKCGLVQVNPQPDLQAVLPYYADSYFKK
                     RTDRGYDNYFSDEIKAEIRRVFTMNLEDIGFFAYEKKVFEKQRPGRALDAGCAAGYFV
                     EYMAERGWNAEGIELSAVAAKPGKARGLRITVGDFLTHSDLIPQSYDFISFWASIEHM
                     HEPARVLLRASELLQPGGRMVLSTCRYGLLARLAGRDWRFMNVPEHLYFFSVKRLTRL
                     ASELGFKVVGKVTYGSGLTTRKDASPFYRFAKRWLDPLVKVLGQGDMMVFHLEKMS"
     gene            8763..9539
                     /locus_tag="PKK76_02555"
     CDS             8763..9539
                     /locus_tag="PKK76_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL67372.1"
                     /translation="MIVPIEDLRREIPLAVEKNKTLSMVTYALSDYGEEVLKILVGSI
                     LEKAGRMDLFDIVYTAAKELVVNATKANLKRALFLKLSLNANDPAQYDVGMNYFRGAL
                     TEEKLRKYKKTFKEHNLPVVVTLYYSMDVVHIKVKNNFPLLDIEESRIRQKFRQATSF
                     TSLLDFFMEHGDDTEGAGLGLTMVGILLDESGVDRHAFTLYSSEKYGETVARLEIPLS
                     PDYVSKRKVFESEMHGKGQSPDQLRQDFKYNYKQFTRNHS"
     gene            9545..10606
                     /locus_tag="PKK76_02560"
     CDS             9545..10606
                     /locus_tag="PKK76_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNL67373.1"
                     /translation="MSHADSKTRARRVWIQASAGSLSRLGLVSDQIAAPSSDQVQIRV
                     RACGLNFADIFAILGLYSATPSGAFTPGLEFSGEIAALGDIAQQKTGFRPGDRIMGLT
                     RFGAYTDLINVDYRYIRKIPSSWSFEQGAAFLAQGLTAEYAISDLGSVKKGETVLVQS
                     AAGGVGLISLFLLEQLGAHAIAVVGSPSKIDLLVSRGVKRERILLRSASTFRKDLRRA
                     APDGIDVCLDAVYGPFFKPQYDALRAGGRYVLFGAASMMPEGKKPNYLRLAFQYLRRP
                     ALDPLAMISDNKSLLAFNLIWLWDQTDRLAGLADRLLTYPWQNNPPFVGKVFSFEKAP
                     DALRFFQSGESVGKVILSV"
     gene            10603..>11197
                     /locus_tag="PKK76_02565"
     CDS             10603..>11197
                     /locus_tag="PKK76_02565"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNL67374.1"
                     /translation="MNADADVVIVGGGMAGLSAGIWAGRLGLKSKIIEKDERAGGQLH
                     WIQDVIPDYPGLELRGTDFARTLCDQAQRSASIETGIAVQKILPFQDAPEAGDLYVLE
                     TTGGILSARAVLISTGLRRRTLPAVRAFHGTGIYYTSQPRDQFKGNSLVIAGGGDGAV
                     ENAILLQAQWKKIYIVHRGAALRARPALADHIRNHAHI"
BASE COUNT         2587 a         2714 c         2951 g         2945 t
ORIGIN      
        1 cccagggttt ccggctttac acgcgacagg ttgccatcac ccattacgag tgtgcagagc
       61 aggctaccca tgcgcaatat tgcagtactg agcgacgacg cggatttgaa cgcgaagatc
      121 gcctcttcgt gcaatagctt tggagacgag ttccaggcgg tgttctttca tgaagagcgc
      181 gcatttgttc aatatctcaa ctatgagctg cctgagattg atattataga tttctcaggt
      241 tccaaagcga gcgcgggcag agcgtttact gagatcaaag atgacccgtg gcttcacttt
      301 ggtggaagca ttatcatcta tgaaaaacaa aaagaagagg atttgctcaa ccgactgcgc
      361 ggtgtgaata tcattgcaca catccagaag agcaaagtgg agttgtatct tccgcgagcg
      421 ctgaatgttt tgtctcaaca cagaagcatt ttgttccagc gcgacttgca cgcactgctg
      481 cagtccaaca tctccggtat gttcgttctg gacaacgatc catttgatgt gactacttac
      541 tcgaacctga tcgcgaattt tctctacaac tccaacttga tcaatctgga ccagaagtcc
      601 aattgcagcg cggccctggt tgaacttctc tacaacgcaa tcgaacacgg gaattgctgc
      661 attggatttg aagagaagaa ggtctggctg gaaaacggcg gagatattct agatctcatt
      721 cgacaaaaag caatgaagcc agagattctg gcaaagaaga tctttttgaa ctatcgcata
      781 acaccggaaa ggtcctattt caccgtccgc gacgaggggg agggatttga ctggggatcg
      841 cgcctggcag aggacaatcc agcagaggca ttgcatggtc gcgggatcat gattgcccgg
      901 aactacctgc agaatgtgca ctacaatgaa aagggaaacg aagtctcttt tcaaatagaa
      961 catttacatg aatcaaatgt aatccctcgc gtttttgcgg atcaggagga agtgacggtt
     1021 caggatggtg aggtgatctt tcgccaggga gaaacgtcca gtcatctcta ctatataatt
     1081 tccgggaaat acgatatcat cgcgaacggg agaaaggttt ctacgctaac accagcggat
     1141 atctttctcg gggaaatgtc attcttactg aacaacaaga gatcggctac tgtgattgcg
     1201 gtgggccgtg gtcagctcat gaaaatttcc aaacaggcgt tcatcaacgc gattcgcgag
     1261 catccgcact atggtatttt tcttgcgcgt cttctcgcgc aaagactcga ccaggtacat
     1321 gaagttggac tttgaaccgg agttgcgcat ttgattagaa cggttctccg cggctttcgc
     1381 cgcgtttctg cagtgatcct gggtcggcct gattttgcaa atctggacca gcggctcttc
     1441 aacgttatgt gctttggtgg aacaatcgtg gcagtattgt ctgcgatcga gaacgcgttc
     1501 attgacgttc cctttgtcct cgtagtaggc agttgcgtgc agacagtcat tctcgcctct
     1561 ggttttgtag tttcgcgtta ctggaatatc tacaggccca tcgtatggcc cgtcatcctt
     1621 gcgattcttg tgtttctagc catgcagtgg ttctggaatg ccggatctgt tggtggagct
     1681 cattacttct tcttcgtgtg tggcatgatg ggtgcagtca tcatgagagg gtggcagagg
     1741 tattttgtta tacttctcta tcttgtggtg atcaacggag ttctttatct ggaatacatt
     1801 cgtccggatt tgattgtgaa ttactcggac cgacaatcgc gttacgtgga cattgccttc
     1861 agtcttagtt tgtgcatgat tctcttcggg cttgtgatcg cgattttttc agggcattat
     1921 caggttcttg tgaatcgggt gcagaggcac aagctggaat ttgtagaaga tctgcatttg
     1981 gcgcgcattc ttcaggaaac tgtattccag catgatcgaa ccctcacgca gggttttgat
     2041 tgcgaaatcg tctatcgtcc gtctgccgcg ctcggagggg atttatacga cctttcgcgg
     2101 cgtcttggga tccttcgcgt gtttatggcg gacatgaaag gtcacggagt caatgctgct
     2161 ttatcgtcta tgctcatcaa gagtgaatgg gttcattcag gccatgcttc gtttagcgcc
     2221 ggtgatgcgc ttgcgaattt gaacaagcag gtagtggccc gatatggcga ttctctggtt
     2281 ctatctgcaa tagttgcaga tctttacgaa gacagcattc atttcgcggc cgccggtcat
     2341 gttcctcagt tcagagtgtt cggcggagaa gttctcaggc ttgaggcgac gggcgttcct
     2401 atcggagtca tggaagattc tcagtattcc acggagagga cggcgcttcc tcgtggatcc
     2461 aggcttgtgt tctttacaga cggtttccat gatcaaccgg atctttcggg taagactatt
     2521 ggcacggact ggatggaaga gatcctgcgt aaatcacagc cgtcgagcgc ggatatggtg
     2581 agagcgctga tcagcaaatt tcacgcgaag actggaacgg agccggggac cactgcggat
     2641 gacctgactt tgattgtgat cggccgctga gtactaatcg aatcgaatca ggatagagct
     2701 tccgtgatct tttaggaatt tgcgaccttc cttgtattcc ggtaaaacgc gttctacttc
     2761 cgcccagaat cgactggagt gattggcgtg gatgaggtga cacagctcat gcacaatcag
     2821 gtagtcgatc acgtattgag gcgcagccag gatcttccag tttagactga gcgatccagt
     2881 atgtgaacag cttccccatc tgcttttcat tccgcgtacg gaaattctgg atggatgctt
     2941 cccgacgatg ggtgcaaatc tggatactgt ctctcggaag attttttccc cgtgtttgga
     3001 atagacagcc gctactttct tcttcgcaat cagagcattt cgggcatgaa ccgttagtga
     3061 ttcatccgtt aggcggcttt gcctgatgcc agggagcaca tgtagtagaa tctcttttcc
     3121 ctgccacggg atcttctctc cgtgcacata acgcagcctt gctgccgatg gacgttcctg
     3181 gtttgcctgt agtttttgac gaatccacgg agctgcctgc aaaagaaatt cgcgtactac
     3241 cctgtcagaa gttcgcaagg gcgctcggac ttcgattaca ttgtccgcca gaacaaacac
     3301 agtgagcctt tttctgcctt tctttctcaa cagacgcaga ccctggaaat ccacgggatt
     3361 catgtagggt caatagggtt tcgcgcattc cttttgcaaa tcattctcgt cttgatctga
     3421 atcaatgttt gtgcaccgaa tctgtgttag tttggaatca ggagtaaacc tgtgatcctg
     3481 tcttctgata ccctttccaa aattgcgctc agcgcgcctc acgccacaag ggtgtttgca
     3541 aaatatagaa tggacttttg ttgtggcgga gggacgagcc tctctgacgc gtgtatcgga
     3601 aaaggcctga attctgaatc tgtcctggcg gagatacagg cggcggctcc actccggcga
     3661 agtgtagagg cggatgctcc attgggggag gtcgtagatc atgttttgtc cacgcatcac
     3721 gaatttaccc gcaacattat ggcagaaatc gaaaagtttt ccgttcgcgt ggcagcggta
     3781 cacggggcgc agcatcccga aatgatcgtg ctccgcgatt tgtttgcgga tttcaaagca
     3841 gaaatggaac ttcatctgtt gaaggaagag cgtgttctgt ttccttacat tcgactgctg
     3901 gagaatggtg ccgagaagga tgcgcccgtc tgtttttcag atgtgggcgg tcccatccgc
     3961 atgatgctct tcgaacatga aaacgccggc gaagaccttc gcaagatgcg agaagtcact
     4021 tctgactaca ctctcccgca ggcagcgtgc aacacctaca ggcttctatt tcataagctc
     4081 caggaactgg aggaagacgt gcacctgcat atgcatctcg aaaacaattt gctctttcct
     4141 cgtgcaatgg agctggctgc gaatccaggt tgacgatcat cgcaaatcgc cctctatggg
     4201 ccggtgactt tcgtaatcca tcggatgctt cggtttctca caattgcatg cgttgctctt
     4261 tgctcctgcc agtggctgat tcgtccggga actgatttcg aaaagagccc gccaccacct
     4321 gagcccgatt actccctgag agaatcctgg gccgctcttc cagaccgcgc agatacagcg
     4381 gactctgtcc cttctgaagc agactggaaa gacgcacagt ccacagcctc cgcagatgtt
     4441 ttctttgttc atcccacttt gtattttggc ggaacctgga atgcggatgt tgccgatgca
     4501 actgtgaacc ggagagtgga tgagaccact cttcgcaagc aggccagcgt ttttaattgt
     4561 tgtgcgagga tctatgcgcc tcgctaccgt caggcgacca tcgctgcatt tctcgacaaa
     4621 aagaacggtg cgccggccct ggatcttgcg tattcagacg tgcttcgtgc gtttgattac
     4681 tatatgattc actacaatca gggcaggcct gtcatcattg cttctcacag ccagggaacg
     4741 cgccatgcct atttcctgtt gaaagatcga tttgatcgaa agcctgctct cagtcgcctt
     4801 gtggcagcgt atctcattgg aatggccatc cctgaacatc cctatgccac cataccgatt
     4861 tgcaagtcgg ccacagaggt tcggtgtttt attacgtatc gctctgcaac acccggaaca
     4921 gaactgaagc gtttgccgca cgatccagag ccaccgtatg cttgtaccaa tccagttcac
     4981 tggaagtcgg acgattctct ggcagaggca gatctcaatc cgggtggggt ttcagcgaac
     5041 tttgaaaaag tagatccaaa tgtttgctct gcgcagtgcc agaatggcgt tcttctgatt
     5101 tcaaagccca aagagtccgg ctatcataat atttctggca actttcacgt ttcagactat
     5161 tcgctttttt atgtgagtat tcgtaagaat gtggcggatc gaatcgccgc cttcagatga
     5221 gtgttcttgt acttgtactc gtcgtcctat tttgttcgat tgctttgcta tttccgcgga
     5281 agcaagccag gagttcctcg cgcatagccg cagttaagcg aaatagtccg gtgattctga
     5341 gacaactggt ctatcgcggt ggagactggg acgccaatcc tggatctctg cgaaacctgg
     5401 cacgtttcgc tagcaactgc gaagcgataa aactggatgt gcgcgccgga gagcctgcaa
     5461 acgggcgaga tctaccgggt tctgattttt ttgtatacgc aaccgggcat cacggttttg
     5521 acctggcagg ctctgatcta acaggatttc tcagttttgt gcgtgcaggt gggttcgttc
     5581 ttgtggacga caatcgcgga atggacgcag ctttcagacg atttgtcgct cagaattttc
     5641 cggaagcaag gctcgaacct gttcgatccg gtgaggaagt gtttagaaag ccgtataacg
     5701 ttggatccat gccaaaaatc atgattcacg atggttcacc ggcagtgttg tatcgcctga
     5761 aggaaggaca ggatttgaat ctgtactaca gctttagttc tgacctgggc gacggctggg
     5821 aggattcatt gcgacacagg gtgccggaag aaactcgcag gctggcgtta gaaatgggca
     5881 cgaacttaat tgcgcttgcg ttcgcctggt ccagcgccgc gcgcgaaaga agagggaatt
     5941 tcgcagcgcg aaattcctcg taggaaacga cgctgctctt cagagcagcg caacttcaga
     6001 ttactttttc agaatgaccc agatggcgtg gatgattcct ggaacgtagc caaagatggt
     6061 caaaagaatg ttgagccaga aatgaaatcc aattccaacc tggaggaaaa ctccgagagg
     6121 tggaagtacg attgcagcga tgatgcgaac tatgtccatg tgtatctcct tattttacta
     6181 cttgaaaggt cgttgagtat tctgacgtat caccatcttc cgtgtgaaca tagatggtga
     6241 tcttccacgt gcccagatac ttatcgtcat gcgtattggg cgtcatccag aagtaaacat
     6301 agttccagtc tgcctgtgta tcaatgtcta tgttgattgg tttgttcttt cgcgtatcgt
     6361 cttgtagttc aaagacagcg ttgagtttgt acacgcccgg tgtttcgata ttggagaaga
     6421 catgaatctt gtctgccgcg cgaaattttg tcttacatcc gctgggtgtt cctgcgttct
     6481 catctacggc gaggcaagcc tttggtttgg atagaattgc ttcctgggca aagagcgcat
     6541 gggaagaaaa gaacagcgcc attgtcaata atagtggttt catagtccct ccagatttga
     6601 gactgatttt gttttgcacg agttcaatta aaaaacgtcc actcggatgg cgtggttggg
     6661 acatacatgc acgcattgtc cgcaaccggt gcaagacttt gcaatgtcag gcagcctgtc
     6721 ttttccgagg gagatcgctc ctttcactgg gcattcgcgc acgcaaatct tgcatgtttg
     6781 cgatttttga cccggagcga ggcgtggttt tctggactcg tggtttctgc agagcgattc
     6841 tgataccgcg gccacgccga gtcgcgggtt ttgcttcttt ctgagtttct tcagcgcgcc
     6901 tgtttcgcac gaatcaatgc acggcatgtc ggggcaaagt ctgcatgcgt ttagatttgg
     6961 atcgagaagt ggaccgctgg ccggtcccat ctggaagagc actccgtatg ggcaggcgac
     7021 gatgcaatcg ccgcattttg tgcacgcctt gttgaatttc tttggatctt tcagtgcgcc
     7081 tggaggacga gcgagtttgc gcggaggagg agcatctgcg ggaggaacag gagcaggcgg
     7141 agtgaagtgt tccggcgaaa gcagatttga aaatgcaagc agttgcttgt cgatggcgga
     7201 gccgacggga gtcttcatga cttcccgcgc gacttctttt acgccttcgc gaaagaactt
     7261 acgtcgatcc atccgatggc ccagcgcttg cctctggact ctttccagga tcgtctccgg
     7321 ggacctgacc ctgaggagtg agtttgtcta cgatgcccgg tagaatctga cccaggaatt
     7381 tggaagcgtt ctcaggagat actcctgcct tctgtgccag agcctggagc tgttcgcttc
     7441 ccaggacatt cttgatttgt tcaggcgaaa tcaatttgtt ttcaccagtt ccaatccagg
     7501 attctgccag gtctccgagc ccttttgtct tcaggtcctg aacgattccg cccactccct
     7561 ttttctggag catgtcgatc actccaccca ccaggttctg attgccttct tttcctgcga
     7621 catcattggc cgcagagatc actttatcga ggaagcccat ccttctttct ggaccatgcg
     7681 ggttgatttg caacccaatt tcgtcgccgg atcgcccccg aaaagggtga atgaatcctg
     7741 aaatcctccg aagatactat atgcaggtac tggaagcacc gaagaaaaaa cgcagagcac
     7801 cgaagaagtc aaaagatccg tttcatcctc ccttgttcga agcgcgtcca ggccacgttc
     7861 gcctggtgcc ttgcaatgca tgtggtggaa tgagctttaa gactcttttt gaaaaggagt
     7921 cttcgctcaa agaagtcttc caggtggttc gctgcaagtg cggactcgtg caggtaaatc
     7981 cgcagcctga tctccaggca gtccttcctt actacgcgga ttcctatttc aagaagcgca
     8041 cagaccgtgg atacgataac tacttctccg acgaaatcaa agcagaaatc cgtcgcgttt
     8101 tcacaatgaa tctcgaggac atcggcttct tcgcatatga aaaaaaggta ttcgagaaac
     8161 aacgccctgg tcgcgctctg gacgcaggct gtgctgcggg atacttcgtc gaatatatgg
     8221 cagaacgtgg atggaatgca gagggcattg agctttctgc tgtggcggca aagccaggca
     8281 aagctcgcgg cctccgcatc accgtcggcg attttctcac gcactcggat ttgattcctc
     8341 aatcgtacga tttcatttct ttctgggctt ccattgaaca catgcatgaa cccgcgcgcg
     8401 ttctccttcg cgccagtgag ctactgcagc cgggcgggcg catggtgctt tccacctgca
     8461 ggtatggtct gcttgcacgc ctggcggggc gggactggcg attcatgaac gttccagagc
     8521 atctgtactt cttcagcgta aagcgcctaa cgcgtcttgc gtcagagctt ggcttcaaag
     8581 tcgtaggaaa agtcacatac gggtctggcc ttacgaccag gaaggacgca tccccattct
     8641 atcgctttgc aaagagatgg ctcgatcctc tcgtcaaggt actggggcag ggcgatatga
     8701 tggtatttca cctggaaaaa atgagttgat cgccggagag ggggctcctt aaaatatcac
     8761 gcttgatagt accaatagaa gatctccgcc gcgaaatccc gctggcagtt gaaaaaaaca
     8821 aaactctgag catggttacc tatgctctct cagactacgg cgaagaagta ctcaagatac
     8881 tcgtcggcag cattctggaa aaagccggca gaatggattt gttcgacatt gtgtacacgg
     8941 ccgccaaaga gcttgtggtc aacgcaacca aggcgaatct aaagcgcgcg ctcttcctga
     9001 aactcagtct gaatgcaaac gatccggcac aatatgatgt tggtatgaat tatttccgcg
     9061 gagctctaac agaagaaaag cttcgcaagt acaagaaaac gttcaaagag cacaatctac
     9121 cggtagttgt aactctttac tattctatgg atgtggttca catcaaggtg aagaataact
     9181 ttccccttct ggatattgaa gaaagcagaa ttcgccagaa gtttcgtcag gcaaccagtt
     9241 tcaccagcct tcttgatttc ttcatggaac acggagatga cactgaggga gcaggccttg
     9301 gtctcaccat ggtgggcatc cttctagacg aatcaggcgt ggatcgccac gcattcactc
     9361 tgtattcaag cgaaaagtac ggggagacgg tggccaggct tgagattcct ctttcgcctg
     9421 attacgtttc caagagaaaa gtatttgagt cggaaatgca cggcaagggc cagtctccgg
     9481 atcagttgag acaggacttt aaatacaact ataagcagtt taccagaaac cattcgtaga
     9541 cttgatgtcc cacgcagatt caaagaccag agcgcgccgt gtctggatcc aggcttctgc
     9601 gggatcattg tccagactgg gtctagtgtc agatcaaatc gcagcgccct catcggatca
     9661 ggttcagatt agagttcgtg cgtgtggttt gaattttgcg gatatctttg caattctggg
     9721 tctttactcc gccactccat ccggtgcttt tactcctggc cttgaatttt ctggagaaat
     9781 tgcagcgtta ggcgacattg ctcaacagaa aacaggattt cgcccgggcg atcggatcat
     9841 gggtctcacc cgttttggcg catacacgga tctgatcaat gtggactatc gttacattcg
     9901 caaaattcca tcttcgtgga gctttgagca aggagctgca tttcttgctc agggtttgac
     9961 tgcagagtac gcgatttctg atctgggttc cgtgaagaaa ggagaaactg ttctggttca
    10021 gtctgctgca ggcggtgttg gtctgatttc tctcttcttg cttgagcaat tgggagcaca
    10081 tgcgattgca gttgtgggaa gtccatccaa gatcgatctt cttgtttctc gcggagtgaa
    10141 gcgcgaaagg atccttctac gcagcgcttc cacattcaga aaggatcttc ggcgtgcggc
    10201 gccggatgga attgacgttt gcctggatgc ggtgtacggt ccatttttca agccgcagta
    10261 cgatgcgctt cgcgccggtg gacgttatgt gttattcggc gcagcaagca tgatgcctga
    10321 aggtaagaaa ccaaactatc tcagactggc ctttcaatac ctgcgaagac cggctctcga
    10381 tcccctggcc atgatttcag acaataaaag tctgcttgct tttaatttga tctggctctg
    10441 ggatcagacg gatcgccttg cggggctcgc agacaggctc cttacgtatc cctggcagaa
    10501 caatccaccg ttcgtaggca aagtgttttc ctttgagaag gccccggatg ctcttcgctt
    10561 ctttcaatca ggcgaaagcg tgggcaaggt cattctgtcc gtatgaacgc ggatgcagat
    10621 gtagtcattg tcggtggcgg catggcagga ttgtccgccg gaatctgggc cggaaggctt
    10681 gggttaaaat ctaagatcat agaaaaggac gagcgcgccg gcgggcaatt gcactggatt
    10741 caagacgtaa ttcctgacta tccgggattg gagctgcgtg gaacggactt tgccaggacg
    10801 ttatgcgatc aagcgcagag atctgcttcg atcgaaacag gcatcgcagt gcagaagatc
    10861 cttccatttc aagacgcgcc tgaggccgga gatctctacg ttcttgaaac aacaggcggc
    10921 attctgagtg cccgcgctgt gctcatctct accgggctca gaagaagaac ccttcctgca
    10981 gtgcgcgctt ttcacggaac tggaatttat tacacgtcgc agccgcgaga ccagttcaaa
    11041 ggaaactctc ttgtgattgc aggaggtgga gacggggccg ttgagaacgc gattctgctt
    11101 caggcgcagt ggaagaagat ctacatcgtg cacagaggag ccgcacttcg tgcaagaccg
    11161 gcactcgcag atcacattcg aaatcatgcg cacatag
//
LOCUS       DAORFF010000034        11181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2619_length_11181_cov_3.865810, whole genome shotgun sequence.
ACCESSION   DAORFF010000034 DAORFF010000000
VERSION     DAORFF010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11181
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2619_length_11181_cov_3.865810"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PKK76_02570"
     CDS             complement(<1..722)
                     /locus_tag="PKK76_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNL67375.1"
                     /translation="MTSLNGIRVLDLSAVFPFPMAAAYLADLGADVIKIENPRLPDPS
                     RKMGSPSGSTHDGSIFVLANRNKRCLSLNLKRPRGMEVFKKLLANTDVLIESFLPGTT
                     SRLGITYDDLRDSFPRLVYCHGSPYGYGQSSRPAHEINIAAEAGLIATTGTASTPVPP
                     GFQPSDVAGAFCAVGGILAALLARHQTGRGQFVNASLFDGAMSMLSLVAAEHLAGGNT
                     DRGKMDVSGMAPGYRVYQTREG"
     gene            complement(719..3145)
                     /locus_tag="PKK76_02575"
     CDS             complement(719..3145)
                     /locus_tag="PKK76_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67376.1"
                     /translation="MGSKPLAILIISDDRAFIEDSAGVFMNLAGAGHAQVQCDFENTQ
                     LDIGRMAWLTDSPVYRNAQIVAQSFLLAHTTPPILVFQASTFQEAVTRITAKHRESEF
                     ALGMIVLDETTVGDLSYERTGLIQAFDDFYSQLGTAGVEWIRSSYSIIAYTAAFPFLS
                     GGAYLPPLRYLYRVRPQDTSLLRADLLCCFMDFYELTFLNTRARRGEKNRDNVTLALE
                     LVAFLNNRSQPREWDCYYYTGSIVSSLIETIEKALYQQNQFCFMGPNEHSLACGAMAN
                     WKLYARPFLIVVTSAMADEFKGTLANLREARAKGFIVVAENREDAWFPFQGTRTQEEN
                     MMDVIKAKKLPAVYLKDSETLATDLQTAFEFYESGEGPVFLFVTQQVLQSKQPLSVAA
                     DQLYPKPKVVVPAPSSPQTEQDFEKIVRLLNEEKTRIIWQVGQLSEEEYNLTIEIADA
                     AGLALCDNLSRPGNVSKYKNGQLVPQYLGTLSLYGFSQRAFRYMHNHGKLNPKDSQVL
                     FFFKSKISQINSPFSESAMKNKLRIGQISANPTHLARFVDFPCAMDSLEFLRKLRRAL
                     NVKPEVRAFREETLKQLPFTTGDIVSKVATQPLHPNYFFRELNQIVEELIVKEGYDYT
                     GVYDVGRNGVSGYRNVARTRPGFSGWYGRALMGDALLSVNALAVTSPVNILAFVGDGA
                     RGVVPDIRASMVENIAHMYRPGAFRKNITIFYMINGLVGLINTYQERMMSHRARRQMR
                     CVSLLDPDVKIKVGDLTVQQRNLYSFDADFIRESLLAPGQLNFFSVITNHNNTGDGMS
                     LLAADNWQEI"
     gene            complement(3269..4525)
                     /locus_tag="PKK76_02580"
     CDS             complement(3269..4525)
                     /locus_tag="PKK76_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67377.1"
                     /translation="MNDWCSGIAAKNLKRRSELVATVGQHSFTWARRIHALSRRPGGV
                     AEVIILNCSLLKNRSAKSPIIAFAHKKIHDAFTAVELLVSREIPMPAQTVIAQGGLFS
                     GMYVYRDWVPDFLKFQPLRFFSETFARILGNKMNRLFRDMNCHPVFRRFRDVPSRKVY
                     ESSRFSGRAISGMTYGEFLEYTRKETQQSISAVERDIRELNKSLIIFPEGKYQHSGAV
                     AKLNGFLMNVATESGHPIAAISQSYDELCPDSKRRIDALLTVSWIEPGETRVALSKKT
                     AAALQTGTAIVASQLIAWLLLQANRENKKEGITGSPAAPDAVNHLRLEFLRMCEEAKR
                     SGVPMDPRLHDRGFAEDRFARFMRANGDYVYEIDSKIVINQKKLRSFSKSERTVNNLE
                     WNKNNIIHLEGQLSAWFASSETSKDL"
     gene            4590..5102
                     /gene="tpx"
                     /locus_tag="PKK76_02585"
     CDS             4590..5102
                     /gene="tpx"
                     /locus_tag="PKK76_02585"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNL67378.1"
                     /translation="MASVTHRGNTIQLDGNLPRPGEKAPGFKVVKDDLSEVGLESFAG
                     KVKVIVALPSLDTPVCQKETREFNQKLGSMKDTVVLVVSGDLPFAMKRFCSTEGLSNV
                     VTGSQFRDHAFSHAYGTFITEGGLKGLSARAVFVVDKNDTVQYTELVPDIGGEPDYNA
                     VSQAVSKLVG"
     gene            5102..5683
                     /locus_tag="PKK76_02590"
     CDS             5102..5683
                     /locus_tag="PKK76_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67379.1"
                     /translation="MTVLSDLRMIFSDWQAGLRDFLGRPAEPSRYYFLKNHLLLCIHA
                     PLFAALAPLPWIRGGHFSYSMRLILPFVLPIFFVALAAVLERIAIYERGPSMEEPRGK
                     NALLYLTLPACSAGIFYFVHPLLGILLTLAFGMFSIVACIRAAMEIWSLSFDRVLFRL
                     AGAFLFLMTPVLALLAIRNVLQSLGILNRLIHG"
     gene            5686..7086
                     /locus_tag="PKK76_02595"
     CDS             5686..7086
                     /locus_tag="PKK76_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNL67380.1"
                     /translation="MKIHITGVGGVAMGNLAAMLKYIGHDVTGSDRDLYPPMSERLKE
                     WKIEAHNFDSANVKGMDLCIIGNVISRGNPEVEEILNGGIPYMSLPQALYEFFLKGRR
                     VIVAAGTHGKTTTTFLTHYILSQAGIKAGLFAGGVRADGHDGFALPESDIFVIEGDEY
                     DSAFFDKGSKFLHYRPRYLILGAVEFDHADIFRNYEEYALPFRRLLRMIPSNGLVVAN
                     QMSDGVRDVLTDYRYSPIEWIANQSKKGVFGFHRRAETLSLDGIGDIRGNRLMGDYNA
                     RNALAASRIALALGVSPKQIRDAVASFPGVMRRQQIRLEANIDGVPVTFIEDFAHHPT
                     AVSETIDAVRQTYPDRSVSVLFEPRSATSHRNVFQKEYGAAFKRADAVYICEIHNKKK
                     VGVKDRLDVKKILAGLKVKTKEFGRDPDHLLTRFTSTFKVGKEGSVVLVLSNGAFGGI
                     YQKIETFLRDRSRRVR"
     gene            complement(7123..7653)
                     /locus_tag="PKK76_02600"
     CDS             complement(7123..7653)
                     /locus_tag="PKK76_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNL67381.1"
                     /translation="MPDTAVRSVNNQQELLQQIDEITRAICQIFHDVPDEYLYSAANP
                     DGWTVEKNMKHIASTNRLMSRWIKAPRFLLTLRGKPKTNLRIEDISATNRPNIRDYGS
                     YEKQGAVPVGRKAELIQEIETSAETLKAAVRKRSEEELGKLSGLWGGMPLGIFAQFAL
                     KHGLHHASVVKARMHG"
     gene            complement(7703..7972)
                     /locus_tag="PKK76_02605"
     CDS             complement(7703..7972)
                     /locus_tag="PKK76_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67382.1"
                     /translation="MPNGTKRKRSISPALDKLIGQMEDLSTDAVDAEVCKRFRLLLET
                     NEINVNADVLKKFTANPQDTDLSVFGDDIRPFLRHFRFMHKRQLP"
     gene            8185..9708
                     /locus_tag="PKK76_02610"
     CDS             8185..9708
                     /locus_tag="PKK76_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67383.1"
                     /translation="MRIRYALAVFVAAILAGCRYDSSCYDLDIICHPWLGLAFYRPLT
                     NPCQYQGGTSRSFYTFLGSAGFGDQARAVCAISDTSILVGGIASGNIPLLQGVTPLNA
                     YTGADDFVIVRLDTAGNVYWYTFVGSAGGTDQLTALTQTLDGGFFAVGSVTANIASLQ
                     GKTPLNAYSASTDILAIKFDQFGNVVWYTFLGGPGIETPTSAAVTIDGGLVINGSGDT
                     NIASLGGQTPLNGYNSLTDVLVMKLSSTGALSWYTFFGSAGTDAGTAVASAPDGSLLI
                     AGNASANVATLQGKTPNNAYVGADDYMIIKLSASGAVSWYTFLGGAGTDIPTGVAVMP
                     DLGAAVTGRANAAVASMQGVTPGAAYAGNFDHMVVRLTPAGNVSWFNFIGTAAASDQS
                     GSVAVYSDSSVLVSGFVAASVPSLQGRSPNNPYTAGDDFLLARYTDTGSLTWYTHLGG
                     AGNDQALSGLTPTSDGGFIVSGFSAATIASLQGKTPVNAFAGVSDMVVIKVKPDGNL"
     gene            complement(9731..>11181)
                     /locus_tag="PKK76_02615"
     CDS             complement(9731..>11181)
                     /locus_tag="PKK76_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL67384.1"
                     /translation="DLRCAFANRAYEGWFGRKPDEMIGSSLDQILGPELYAKNKPYVE
                     RALAGEPQQFERTLIKPNGEIGYTWAQYVPHVHDGEVQGMIVLISDITEPRKAESRKR
                     ELEAELVQARKLESLGTLAGGVAHDFNNILGIILGHASLLKMSVKEPELTERLSTIEN
                     AAMRGGALVNQLLTFARKSETSLSLVSVNDTVRETVKLLSATFPRNIEISVQLSENLP
                     SVMGNATQIQQALMNLCTNARDAMPDGGKLTISAKIADPSIIAAEFPKAHFSDFLLIE
                     VKDTGVGMDTETKRRIFEPFFTTKGPGRGTGLGLSVAYSILKEHRGLFVVDSEPGQGS
                     TFRIYLPIESNTTTATGLTIETDRSGKDTILIIEDEQMLRASLEETLVTQGYKVFTAV
                     NGEQGVEVFQQHKDDITVVICDLGLPGLSGHEVIKKIHANRPGTRIIATSGFMDSEAL
                     QKLNQSGNPHILHKPYRLAELISAVRSEVNGI"
BASE COUNT         2525 a         2774 c         2898 g         2984 t
ORIGIN      
        1 aatccttcgc gcgtttgata cactctgtat ccgggggcca taccagaaac atccatcttt
       61 cctcgatccg tatttccgcc tgcgagatgt tcggcggcaa ccagcgagag catagacatt
      121 gcgccgtcaa agagggatgc attcacaaat tgcccgcgcc ctgtttgatg gcgcgccagg
      181 agtgctgcca ggattccacc tacagcgcaa aaagcacctg caacatctga aggttgaaat
      241 cccgggggga ctggcgtgga cgcggtcccc gtagttgcaa tcagacctgc ttctgctgca
      301 atgttgattt catgcgcagg ccgcgagctt tgcccgtaac cgtacgggga tccatgacag
      361 tagacaaggc gcggaaagga atctcgcaaa tcatcgtacg tgattcccag gcgagaagtg
      421 gttcccggaa gaaaactttc aataagaaca tctgtgtttg caagtagctt cttgaagact
      481 tccattcccc tgggtcgctt gagattcagc gaaaggcatc gtttgtttcg attggccaga
      541 acgaagatgc ttccatcgtg ggtgctcccg gaaggtgagc ccatcttacg cgatggatcc
      601 ggaagtcgcg gattttcgat cttgattacg tcagccccga gatccgccag ataggccgca
      661 gccatgggaa aaggaaacac agcagataga tcgaggaccc gaattccatt cagcgatgtc
      721 atatttcctg ccagttatcg gctgccagta gactcattcc atcgcccgta ttgttgtggt
      781 tggtgatcac agaaaagaag ttcaattgtc caggagccag aagcgattcg cggataaaat
      841 cagcgtcaaa cgagtagaga ttgcgttgct gaacagtgag atcccctacc ttgatcttta
      901 catccggatc caacaaagaa acgcatcgca tttgtcttcg cgcacggtgc gacatcattc
      961 gttcctggta ggtattgatc aatccaacga gaccgtttat catatagaag atagtgatgt
     1021 tctttctaaa tgcaccgggg cgatacatat gcgcgatgtt ctccaccatc gacgcgcgga
     1081 tatcaggcac gacccctcgc gcaccgtcgc ccacaaaagc cagaatattt accggactgg
     1141 tgactgccag agcattgacg cttaagaggg cgtctcccat gagtgcgcgg ccataccagc
     1201 ctgagaaacc agggcgcgtt cgagcaacat tgcgataacc ggaaacacca ttgcgcccta
     1261 catcatagac tccggtataa tcatatcctt cttttacaat cagctcttct acgatttgat
     1321 tgagttctct aaagaaatag ttcggatgca atggttgagt cgccactttg ctgacaatgt
     1381 ctccggtagt aaaagggagt tgcttgagcg tttcctcgcg aaaggccctt acttccggtt
     1441 ttacattcag cgctcttcgc agctttcgca gaaactcaag cgaatccata gcacacggaa
     1501 aatcaacgaa acgagccaga tgggtcgggt ttgcactgat ttggccaatt cgaagtttgt
     1561 tcttcatggc actttcggag aagggagaat tgatctgact gatcttgctt ttgaagaaaa
     1621 acagaacctg tgagtctttg ggattgagtt tcccgtggtt gtgcatgtag cgaaacgctc
     1681 gttgtgaaaa tccatacaac gacagagtcc ccaggtattg aggaacaagc tggccgttct
     1741 tatattttga aacatttcct ggtcttgaaa gattgtcgca tagcgccagt ccggcggcgt
     1801 ccgcaatttc aatcgtaaga ttgtattctt cttcgcttaa ctggcctacc tgccagatga
     1861 tgcgcgtttt ctcctcattg aggaggcgca ctatcttttc aaaatcctgt tccgtctgcg
     1921 ggcttgaagg cgcaggcacg actacttttg gttttggata caactgatcg gcggccactg
     1981 acagaggttg tttgctttgc agtacttgct gcgttacaaa aaggaaaacc gggccttcgc
     2041 cactttcgta gaattcaaag gcggtctgca gatctgtggc aagtgtttct gaatccttga
     2101 gatatacagc cggaagcttt ttggctttga tcacatccat catattctct tcctgggttc
     2161 ttgtgccctg gaaaggaaac catgcgtctt cacgattttc agcgactaca atgaagcctt
     2221 tcgctctggc ttcgcgcaga tttgcgaggg tgcctttgaa ttcgtcagcc atggcactcg
     2281 tcacaacgat gaggaagggt cgcgcgtaga gtttccagtt tgccatagct ccgcaggcaa
     2341 ggctgtgttc gttaggaccc ataaagcaga actggttttg ctggtagaga gctttctcta
     2401 ttgtctcgat gagtgaagat acaatagacc cggtgtagta atagcagtcc cattcgcgtg
     2461 gttgcgatct gttatttaga aaagccacaa gctcaagggc cagtgtgacg ttgtcgcgat
     2521 tcttttctcc cctgcgcgcg cgcgtgttca ggaatgtgag ctcataaaaa tccatgaagc
     2581 aacagagcag atcagctcgc aggagagatg tgtcctgtgg ccgcactctg tacaggtagc
     2641 gaagaggggg aaggtaggcg ccgccgctta aaaatggaaa cgctgccgtg tacgcgatga
     2701 ttgaatacga agagcggatc cattcaacac ctgcagtgcc cagctgactg tagaaatcgt
     2761 caaatgcctg gataagcccg gtcctttcat acgatagatc tccgactgtg gtttcgtcga
     2821 ggacgatcat tccgagagcg aattcgcttt cgcggtgttt tgcagtgatg cgcgtgacag
     2881 cttcctggaa cgttgatgcc tggaagacca gaatgggcgg ggtcgtatga gcgagtagaa
     2941 aactctgggc tacaatttga gcatttctat acactggact atctgtaagc cacgccattc
     3001 ttccgatatc gagctgagta ttttcaaaat cgcactgaac ctgcgcgtga cctgctcccg
     3061 caaggttcat gaaaactccc gccgagtcct caatgaatgc acgatcgtcg ctgatgatta
     3121 gaattgctaa aggtttgctt cccatttttt ggccctgaat ctgctatcat gggagagttt
     3181 ctctggtcaa gcaacgaaag tttaacattt ccgacttctt cgcgtcctga tcttgaaata
     3241 cgggcacgta gtcgatgagg taatccgctt agagatcttt tgatgtttcc gaggacgcaa
     3301 accaggcgga cagttgccct tccagatgaa tgatattgtt tttattccac tcgagattgt
     3361 ttacggtgcg ttctgattta ctaaaagatc ttaacttttt ctggttaata actattttcg
     3421 aatcgatttc gtacacgtag tctccgtttg cgcgcatgaa tctggcaaaa cggtcctccg
     3481 cgaatccgcg gtcgtgcaat cgtgggtcca ttggaacacc agatcttttc gcttcttcgc
     3541 acatgcgaag aaattctagt ctgaggtggt tgactgcatc cggagccgca ggagaccctg
     3601 tgattccttc tttcttgttt tctctgttgg cttgcagcag aagccatgcg attaactgac
     3661 ttgccacaat tgcggttccg gtttgaagtg cggccgctgt cttcttgctg agagcaactc
     3721 tagtttctcc tggttctatc caggaaacag tgagtaatgc atcgattctt cttttggagt
     3781 ccggacagag ttcatcatag gattgagata tagccgcgat gggatggccg gactctgtgg
     3841 ccacgttcat cagaaatccg ttcagttttg cgacagcacc gctgtgctgg tatttgcctt
     3901 ccggaaaaat gatcagagac ttgttgagtt ctcgaatgtc gcgttcgact gcgctaatcg
     3961 actgttgtgt ttctttcctc gtgtattcca ggaattcgcc gtacgtcatt ccgctgatgg
     4021 ctcgccctga aaaccgcgag ctttcgtata ccttgcgtga gggaacatct ctgaatcgtc
     4081 tgaacaccgg atgacaattc atatcgcgaa aaagtcgatt catcttgttt ccaagaatgc
     4141 gcgcaaatgt ttccgagaaa aaccgaagtg gctggaattt tagaaagtcg ggaacccaat
     4201 ctctgtagac atacatgccg ctgaacaatc cgccctgagc aatcacggtt tgcgcgggca
     4261 tgggaatttc gcgcgaaaca agaagctcga ctgcggtgaa tgcgtcgtga atctttttgt
     4321 gcgcaaatgc tatgatgggc gactttgcgg aacgattttt caacaatgag caattgagaa
     4381 tgatgacttc agctaccccg ccgggccttc tagagagcgc atggatgcgc ctggcccagg
     4441 tgaacgaatg ctggcccacg gttgcgacca gttccgagcg ccgcttcaga ttctttgctg
     4501 caatgcccga acaccaatcg ttcataaaaa gactggataa tccagggcct ttttgggaat
     4561 actatcaaaa aaaaatttag gagggtagca tggcttctgt tacacatcga gggaatacaa
     4621 tccagctgga tggtaatctc ccccggccag gcgaaaaagc tcccggcttc aaggtcgtta
     4681 aagacgatct ttcagaagtt ggccttgagt ctttcgctgg caaagtcaaa gtgattgtcg
     4741 cgcttccatc tctagacaca cccgtatgtc agaaagaaac ccgtgagttc aatcagaaac
     4801 tcggttcaat gaaggacaca gtggttctcg tagtatctgg agatctgccg tttgcgatga
     4861 agagattctg ttccacagaa gggttgagca atgtagtgac tggttcgcag tttcgcgacc
     4921 acgccttttc gcacgcttac ggaaccttca tcacagaagg cgggctaaag ggattgtctg
     4981 cgcgcgcagt gttcgtagta gataaaaacg acacagtgca atatacagaa ctcgtgccgg
     5041 atatcggtgg cgagccagat tacaacgcag tcagccaggc agtgagcaag ctggtaggtt
     5101 gatgaccgtt ctctcagatc ttcgcatgat cttttcggac tggcaggccg ggttgagaga
     5161 ttttctcggc cggcctgctg agccttcacg ctattatttc ttaaagaatc atctgctttt
     5221 gtgcattcac gctccgcttt ttgccgcact tgcaccgttg ccctggattc gcggcggcca
     5281 tttctcctat tctatgaggc tgattcttcc ttttgttctg cctatcttct tcgtcgcgct
     5341 tgcagcggtt ctcgaacgga ttgccatcta tgaaaggggg ccttcgatgg aagagcctcg
     5401 tggaaagaat gcacttctgt atttgacttt gcccgcctgc tctgccggca ttttctattt
     5461 cgttcacccg ctgcttggaa ttctactgac tcttgctttt ggaatgtttt ccatcgtcgc
     5521 ctgcattcgc gctgccatgg aaatctggtc tctttcgttt gaccgggtgc tctttagact
     5581 tgcaggtgca tttctattct tgatgactcc cgttctcgct ctccttgcca ttcgcaacgt
     5641 gcttcaaagc ctgggaattt tgaaccgtct catccacggg taactatgaa gattcacata
     5701 accggagtcg gcggagtggc catgggaaat ctggccgcga tgttgaagta tatcggtcac
     5761 gatgtgacag gttcagatcg cgatttgtat ccgcccatgt ccgagcgctt aaaggaatgg
     5821 aaaattgaag cgcataactt tgattcggcg aacgtcaaag gaatggatct ctgcatcatc
     5881 gggaacgtta tttctcgcgg gaatcctgag gtggaagaaa ttctgaatgg cggcattccg
     5941 tatatgagtc tgccgcaagc gttgtatgaa tttttcctga agggaaggcg cgtcattgtc
     6001 gcggccggca cgcacggcaa aacaactaca acattcctga ctcactacat tctgtctcag
     6061 gcagggatca aagccggttt gtttgcaggt ggagtaagag cggacgggca cgatgggttt
     6121 gctctccccg agtcagacat attcgtcatc gaaggggacg agtatgattc tgcttttttt
     6181 gacaagggtt caaagtttct gcactatcgt ccgcgatacc tgatactggg tgcggttgaa
     6241 tttgaccatg ctgatatctt tcgcaactac gaggaatacg cacttccatt tcgacgccta
     6301 ttgcggatga tcccgtcgaa cggtctggtg gttgccaatc agatgtcgga cggcgttcgt
     6361 gacgtgctta cggactaccg ttattcgccc attgaatgga tcgcgaatca atcaaagaag
     6421 ggagtgttcg gttttcaccg gcgcgcagaa acactgtctc tcgatggaat cggggacata
     6481 cgcggcaaca gattgatggg cgattacaac gctcgaaacg cactggcagc ctctcgcata
     6541 gccctcgcac tgggtgtatc cccgaagcaa attcgtgacg cagtcgcctc ttttccgggg
     6601 gtgatgcgac gccagcagat tcgtctggag gcaaacatag acggagtgcc ggttactttt
     6661 attgaagatt ttgcgcatca tccaaccgcg gtgtctgaaa caatcgatgc cgttcggcaa
     6721 acatatccgg atagatctgt ttctgtgctc tttgagccgc gcagtgcgac ttctcatcgc
     6781 aatgtgtttc agaaagagta cggcgccgct ttcaagcgcg cagacgcagt ctacatctgc
     6841 gaaattcaca ataagaagaa agtgggcgtg aaagatcgcc tggatgtgaa aaaaatctta
     6901 gcgggtttga aggtgaagac aaaagagttt gggcgtgacc cggatcacct gctgacacgc
     6961 ttcacatcca cattcaaggt gggcaaagag gggtccgtgg tcctggttct ttctaacggc
     7021 gcattcggag gaatctatca gaagatagaa acattcttac gagatcgttc gcgtcgagtt
     7081 cgctaagact ccagtgtctc aagcgcaggc ctgcgttatg tgttagccgt gcatgcgtgc
     7141 tttgactaca gaagcatgat gcaggccgtg tttgagcgca aattgcgcga agatgccaag
     7201 tggcattccg ccccagagtc cggacaattt gcccagttct tcttcacttc tttttctcac
     7261 cgctgctttt agagtttctg cggatgtttc aatttcttgg atgagttcag ctttgcggcc
     7321 aaccgggact gctccctgtt tttcgtagga tccgtagtca cggatgtttg gccggtttgt
     7381 ggcagagatg tcttctatgc gcagatttgt ctttggcttg ccgcgaagcg tgagcaggaa
     7441 gcgcggcgct ttgatccagc gactcatcaa acgattggtg ctcgcaatgt gcttcatgtt
     7501 tttttcaacg gtccatccat ctggattcgc ggcgctatac aggtactcgt ctggcacatc
     7561 atgaaagatc tgacagatgg cccgagtgat ttcgtcgatt tgttgcagga gctcctgctg
     7621 gttatttaca ctgcgtacgg ctgtatctgg catacgttca atttgattcg atttgagcgg
     7681 acgaaaagcc tttttttgac gatcagggga gctggcgttt gtgcatgaag cgaaaatggc
     7741 ggagaaacgg gcgaatgtcg tctccaaaaa cggacagatc cgtatcctga gggttcgctg
     7801 tgaatttctt gagcacgtcc gcgtttacgt tgatttcgtt tgtttctaga agcagtctga
     7861 atcgtttaca aacttcagcg tctacagcgt ctgtagacaa gtcttccatc tgtccgatca
     7921 atttgtccag ggctggcgaa atactacgct ttcttttggt accatttggc attatgtgca
     7981 ggaaaaaaaa atcgctcccg gggtcaatta ctacgaattg cccgatcctc gcggcgctgc
     8041 atcgcatgga cgatcttttc tgatagtggg ctagcaggtt tttgcagctt cctatgattt
     8101 ttcttatgcc cgaaatggaa tcctgagctc ccgggcgtta taaagacaat ttgactgcct
     8161 ggacctggag actaaaatag ttagatgcgc atacgttacg cattagctgt gtttgtagcg
     8221 gcaatactcg ccggatgcag gtacgattcg agctgctacg atcttgacat aatttgccac
     8281 ccctggctgg gtcttgcctt ctacagacca ctgaccaatc catgtcagta ccagggagga
     8341 acttcgcgct cgttttacac gttcctggga agcgcgggtt ttggagatca ggcgcgcgcc
     8401 gtgtgcgcaa tttcagacac cagtatcctg gttggcggaa ttgcaagcgg caacattcca
     8461 ctgcttcagg gcgttactcc cttgaatgcg tacaccggtg cggacgactt tgtcattgtt
     8521 aggttagata cagccggcaa tgtttactgg tacacgtttg tcggcagcgc gggtggaaca
     8581 gatcagttga ctgcgctgac tcagacgctg gacgggggct tttttgcagt cggatcggtc
     8641 actgcgaata tcgcatcgct tcaagggaaa acacctctca acgcttattc cgccagtaca
     8701 gacatactcg caatcaagtt cgatcaattt gggaatgtag tttggtatac atttctcggt
     8761 ggtcccggga tcgaaacgcc caccagcgcg gccgttacga tagatggggg ccttgtgatc
     8821 aatggcagcg gagatacaaa catcgcctct ctgggcggac aaacgccatt gaatggttac
     8881 aacagtttga cggatgttct cgttatgaag ctttcctcta ccggggcgtt gtcctggtac
     8941 acgttctttg gtagcgcggg cacggacgca ggcactgccg tggcatctgc gccggacggc
     9001 tcgcttttga ttgcgggcaa cgcttctgca aatgtggcca ctctgcaggg gaaaacacct
     9061 aacaacgcgt atgtgggcgc agatgattac atgattatta aactctccgc gagcggagct
     9121 gtcagctggt atacatttct gggtggtgcg ggtacggaca ttcctacggg tgttgctgtg
     9181 atgccagatc tgggcgccgc agtgactggt cgcgcgaacg ccgctgtggc gtccatgcag
     9241 ggtgtgactc ccggggccgc atacgcgggc aactttgatc atatggtcgt acgcctcacg
     9301 ccggccggga atgtatcctg gttcaatttc attggaacag cggccgcgag tgaccaatcc
     9361 ggatcagtag cagtctacag tgacagcagc gtgctcgtaa gtggtttcgt ggccgcatcg
     9421 gttccgagtc ttcaaggccg ttcgcctaat aacccgtaca ccgccggtga cgatttcctt
     9481 ctggcccgct atacagacac aggatctctc acgtggtaca ctcatctcgg cggggcaggg
     9541 aatgatcagg cactctccgg gctcactcca acatcagatg gagggttcat cgtgtcgggc
     9601 ttttccgcgg caacgattgc ttccctgcaa ggcaagacac ccgtcaacgc cttcgcgggt
     9661 gtaagcgaca tggttgtgat caaagtgaag ccagacggga atttgtaaat ttccggcatg
     9721 tctgtacggg tcaaatgccg ttgacctctg atcgcacggc agagatcaat tcagcaagtc
     9781 gatacggttt gtgcaggata tgcggatttc cggattggtt tagtttctgc aacgcctccg
     9841 aatccataaa cccgctcgtc gcaatgatac gagtgcctgg ccgattcgca tgaatcttct
     9901 tgatcacttc atggcccgag agccccggca ggcccaggtc gcagatgacc actgtaatgt
     9961 cgtccttatg ctgctggaat acttcgactc cttgttcccc gttcactgcg gtgaagacct
    10021 tgtatccttg cgttacgaga gtttcttcca gagacgcgcg cagcatctgt tcatcttcaa
    10081 tgatgagaat tgtgtctttc ccggatctgt ccgtttctat tgtcaatccg gtcgcggtcg
    10141 ttgtattgct ttcaattggc aggtaaatgc gaaatgtcga accctgtcct ggctcactgt
    10201 cgactacaaa cagtccacga tgctctttca ggatggaata tgcgactgac aatcccagac
    10261 cggttccacg tccgggtcct ttcgttgtga agaacggttc aaagatcctt ctctttgttt
    10321 ctgtatccat gcccacgcct gtgtccttca cttctatcaa aagaaaatca gagaagtgag
    10381 ctttcggaaa ttcagcggca atgatagaag ggtccgcaat tttcgccgaa atggtcagct
    10441 tgcctccgtc cggcattgca tcgcgcgcat tcgtgcagag gttcatgagc gcctgctgga
    10501 tttgtgtcgc gttccccatt acagaaggaa ggttttcgga aagttgaacc gatatctcta
    10561 tattgcgtgg gaaggttgca ctcagtagtt tcacggtttc gcgcaccgta tcattcacag
    10621 atactagaga cagacttgtt tcgcttttgc gtgcgaatgt aagaagctga tttaccagcg
    10681 cgccacccct catggccgca ttttcaatcg tgctcagtct ctccgtcagc tctggttctt
    10741 tcacgctcat cttcaaaaga gatgcatgtc ccagaatgat ccccaggata ttgttgaagt
    10801 cgtgcgcgac gccacctgcc agagttccaa ggctttccag ctttcgcgcc tgaacgagtt
    10861 cggcttctag ttctctctta cggctttccg cttttctggg ttcagtgata tccgaaatca
    10921 agacaatcat tccctgcact tcgccgtcgt gtacgtgtgg aacatactgg gcccaggtat
    10981 aaccgatttc gccattcggc ttgatgagtg tacgttcaaa ttgctgtggt tcacccgcca
    11041 gcgctctttc aacgtagggc ttgtttttcg catacaattc cgggcccagg atctggtcca
    11101 gggacgagcc aatcatttcg tcgggttttc tgccaaacca gccttcgtat gccctatttg
    11161 cgaaggcaca cctgagatcc c
//
LOCUS       DAORFF010000035        11135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2637_length_11135_cov_3.710650, whole genome shotgun sequence.
ACCESSION   DAORFF010000035 DAORFF010000000
VERSION     DAORFF010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11135
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2637_length_11135_cov_3.710650"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PKK76_02620"
     CDS             complement(<1..1098)
                     /locus_tag="PKK76_02620"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNL67385.1"
                     /translation="MNLSIPKEIVAGEKRVAVVPESVKKFIASGFQVRVEKDAGAAAG
                     FSDEDYKAAGAEIISTAGDLYSQADVLVKVQKPIQHPASGKNELEMLKAGALYVGFFY
                     SLSNADLVKLAATNKVNVLSMDAVPRITRAQRMDALSSQTNLAGYKAVLMAANSLTKI
                     FPLMMTAAGTVQPAKVVIIGAGVAGLQAIATAKRLGAVVEVSDVRPAVKEQVESLGAK
                     YIEPPVQDNLEGQGGYAKEASAEFLRKQQEILGNHIAAADVVITTALVPGKKAPVLVT
                     EEMLVRMRSGSVVVDLAAEQGGNCVWTDPGKTITKNGVTVIGETNVVSHVAFHASQLY
                     SRNVLALMEVVSKEGKIALNLEDEIVKGSLIT"
     gene            complement(1157..1993)
                     /locus_tag="PKK76_02625"
     CDS             complement(1157..1993)
                     /locus_tag="PKK76_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNL67386.1"
                     /translation="MLRSIPSKSQIFLLGALILTGSCITEEQAARFRPGTRSSREALR
                     PDDFQIQPNYTDEERAVPIMLAPLPDVVPQVFILSDLPPAGNQMRQASGVAFSTGYLA
                     TSYYHRRRGKPGYTCSPSFVYNSLNGGKDEGISVLDALDFLHARGCPTEQLMPFNEKD
                     FFRKPGPDALQNADAFRVRGYSRIDFRDFDQVHAHLLQGSVLIVSMKITENFVDLREP
                     LWTPAGKFVAIHTVGVIGYDITRQEVLIQNSAGGTWGHFGTARMPWIWFQRLTEKAFV
                     LF"
     gene            complement(1942..2418)
                     /locus_tag="PKK76_02630"
     CDS             complement(1942..2418)
                     /locus_tag="PKK76_02630"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNL67387.1"
                     /translation="MHIALYRPEIPPNTGNIARLAVCTDTPLHIVGEPSFSMDEKAVR
                     RAGLDYWAQLDLKRHADYEEFYASLASIGVPLVAFSRFATDSMWDFTFSGQEALLFGQ
                     ETAGLPQEITEHVRKHGRLLRIPVGEKCRSLNLSNSVAIACYEVFRQNRRSFSWAL"
     gene            2474..3694
                     /locus_tag="PKK76_02635"
     CDS             2474..3694
                     /locus_tag="PKK76_02635"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNL67388.1"
                     /translation="MNYSAQRLNIVEPSPTLAISAKAAELKAAGRDVIGFGAGEPDFD
                     TPDHIKAAAQKAMAEGQTKYTPVGGTAKLKKAVVDKFKRENGLSYEPKEVIVSTGGKQ
                     VLFNLFLAYLNPGEEVIVPAPYWVSYADIVRIVGGVPVIVQCPASQNYLIQPSQIEEA
                     VTPRTRAILLCSPSNPTGSVYSEAELRAIAAVLEKYPDILIISDDIYEHILFDNRKFT
                     NLAMLFPEWKDRIFIANGVSKSYSMTGWRIGYGAGNRALIEGMETMQSQSTSNPSSIS
                     QAAALAALEGPQECVSIMRVAFEKRKNIVMQKFSQVPEITLSAPGGAFYVFPNLDRVF
                     ALPGFQKLRAASKDRSLSRILSAHLLEKYDVAVVPGIAFGDDSGMRLSFALGEDALIK
                     GLDRIAKMVADLRS"
     gene            3691..4692
                     /locus_tag="PKK76_02640"
     CDS             3691..4692
                     /locus_tag="PKK76_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNL67389.1"
                     /translation="MKPLLGDVREWLKELDLPEPTNILPLQKDLSTRTYYRLTLNEVC
                     ELGNSLVLCVHSGQYEATISDFVSISRQFKEAGVAAPDVIAQKQHWLLQTDAGSIDLR
                     QHCLEWIEKGDTQSIEITYERLLDQLVRLQAVTPSHPVSGRFFDFDKLHFEMEHFALH
                     VAQAGQKFAREYFYSFEFQMFLKTICEKLAQSSSKVFVHRDLHSKNVMRSSENLVWID
                     FQDARSGLRYYDLASLLYDPYLPLSRDFRTRMLSSYAKKSGKAVEENLFYMQSFQRLV
                     KAIGTYMSVIHTRQDRDYLGFLRQAAIHLEEVVQLGGFPDHTFLFVRGMQKFSELEF"
     gene            4692..5402
                     /locus_tag="PKK76_02645"
     CDS             4692..5402
                     /locus_tag="PKK76_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNL67390.1"
                     /translation="MNAFLPAAGFGTRMGDLTRFIPKPLLPVGGVPMIYYALFQLWRW
                     KVDRVVVNLHFLKEKIERELSGFKAMEIIFSREDPILGTAGGIRNALPLFSSDAIVVL
                     NPDSIVFTDRSLEQITSAASSSWDSLLGIAPLPQGSANTPLSWKEGQLSFEESGSYYY
                     MGASVLKLSQFDHVASGYSELGSLWREQAYEGRLEGFLYEGNLLDAGNASEYRSLPEQ
                     IVPGHLAASWKEFIKNCK"
     gene            5393..6436
                     /locus_tag="PKK76_02650"
     CDS             5393..6436
                     /locus_tag="PKK76_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67391.1"
                     /translation="MQIILLLVFAVPLFAETPIQNVSPATPTTPAKPDSRPSKEARTR
                     AETDLELKRLTASLDAAFRLQKKFEIPGLLEDLKKLAPDSAQNFYFQALDAYTRGEKL
                     KALALIKKSISKDPLLTPAYTIGGLIYADGGKDQSALPFFERAVQQSPYDPIYLLNLA
                     LCKNRLGMKADALSLLDQALRARANLAEAMYWKGVLLAENGQNAEAQKYARGAVDAGL
                     KNVSAYRLWLQLAFEAADDVDVARAVSELSQDARESARWRLAAEYSFRFGDFRKAVSL
                     LDRAFQIDEGMPEDRRMYVRCLFAEGQDPMPWIRSQRLSDAERQELRTLVDLLQNTPG
                     IVQSRDPLVRPAK"
     gene            complement(6457..7545)
                     /locus_tag="PKK76_02655"
     CDS             complement(6457..7545)
                     /locus_tag="PKK76_02655"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNL67392.1"
                     /translation="MKARLVLVCFVATCALWYLLPNPGSAEETAIPSYPAANPGKATI
                     LFGGDTHFQWGIEELIEKEDALAPVREMQRTFEKADFRILNVETAITDKGEPASGRSH
                     IFRASPGSIKTLTPLKLNLAILANNHAMDMGPEGLEDTRQNLRKAGILTAGVGANAKE
                     ASLPVLFRIGDIRFAVVAASAIGDERMWADDKTPGIAKPEQILAQIQAARTLADHVIV
                     SLHWGMEYNPYPMPDQRPLAQQFIQAGASAVIGHHPHVAQGIETSKNTVICYSLGNFL
                     FGSSNPFQTDNMIVLMEFEAKKPGVRGLRVIPITGHYRESGHAPRPLHTTELHSFFPD
                     LWTQSTKINPSMSSRIIEEEDGIRINVD"
     gene            7660..8640
                     /gene="sppA"
                     /locus_tag="PKK76_02660"
     CDS             7660..8640
                     /gene="sppA"
                     /locus_tag="PKK76_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNL67393.1"
                     /translation="MRKVICIALIASLTGCFNKIVLAPFGASGDDLELREHVLYGRAS
                     TACFSGFSSRTKVLIVPVQGVIGADSMLSRGGTSVAGIKRTLEIAEQDPEIAALILLI
                     DSPGGTVSASDQIHRLLIDFSAKTKKPIYAHVDGVGASGAYYIASSALEINASSASIV
                     GSIGVILQSFSVQGLMQKIGIEYRSLKTGKNKDSLSPFRELREDEKDFFMKQLTQAYE
                     IFLRRIMDGRKDRISEDKLRQLADGSVYSAADAKQYGLIDSVSYIEDYLADVSKRMDL
                     HDPLFVAYLPPGKSYSLYNVRSDRRASWMSLLEMPDHPTAAMYYLWDGGF"
     gene            complement(8649..9491)
                     /locus_tag="PKK76_02665"
     CDS             complement(8649..9491)
                     /locus_tag="PKK76_02665"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNL67394.1"
                     /translation="MKHTLIALALVLGLAACKQGSQVKESESTVARTTFITGEVTLHR
                     GEADQQLKVGDMLQQGDRVSTAAASTAEIFIKGQGIVRLSESTELTLASLQENLKTKI
                     DLKSGSSAFFLRKMDRTGEFTVQSPTAVAGVRGTAFMVSVDSPTESRVALYDGAVAVK
                     NDKGKEVVLSEPGELSIRSGQDISDKAVRPLSKESLAALKKLAVFQRNTIMEYNSLLD
                     EIKSSDAIKGVEINGSTDDKIAQLKDETARPEKGERVRRADENTIKRDTKKDPLKIEP
                     NKTF"
     gene            9581..11041
                     /locus_tag="PKK76_02670"
     CDS             9581..11041
                     /locus_tag="PKK76_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67395.1"
                     /translation="MDPAMLARDTEEIQRIEGFKRGYLPVLEAQSAHARAGQQLLTSN
                     ALTPEEKKVLSQALPELDKLLSSLQTLTRQMTMDLNPNNIAKEAARDYHAFFKGDVMD
                     HQMSFIVFFKTYMHLTRFLKSLSDSWKRIRDLITESKLADNSEFRKQLLHFRVGPVLA
                     DIEALRLFCMRMATILQITRDPLTSRDVASTGKYREDIAYQWSTVFTENLEALIHPTG
                     PDWDSMGIATARAVTNAPPQAPPVGAAAYRLNAGGTQSWNNSAYYYFTYDPKLMEGER
                     ARLTNIVHIDTHMGADQNVMRSERILKYTRKVAHPDVHENANVELEYTGFLHSFFNLV
                     IEISTLNMGIPQKTRDLFLFHLGPQSFYYLTRRFLQELDTGTIHRKSGRGTSMEKFVP
                     GELLKKIILEWWQAHILNVVGMERDDLSQYRMICKLVKDTYDRLSAKAMAEYDQLPEN
                     EKVGKNRAEIIRENLNKWLGPTNIIVFRRFLKSGTP"
BASE COUNT         2669 a         2799 c         2834 g         2833 t
ORIGIN      
        1 cgtgatgagg ctgcctttta cgatttcatc ttccaggttc aaggcgatct tcccttcctt
       61 ggacacaact tccatgaggg cgagaacgtt ccttgagtaa agctggcttg cgtgaaaagc
      121 aacatgcgag acgacattcg tctctccaat cacggtcact ccattctttg taatggtttt
      181 accgggatca gtccacacgc agtttccgcc ctgctccgcg gcgagatcta caaccacaga
      241 tccagaacgc atgcgaacga gcatttcctc tgtcacaaga acaggcgcct tcttccctgg
      301 tacaagcgcg gttgtaatca caacgtccgc agctgcgata tggtttccta gaatttcctg
      361 ttgcttgcga agaaactctg cagatgcttc ttttgcataa ccgccctgtc cttcgagatt
      421 gtcctgcaca ggcggttcaa tgtattttgc accgagactt tctacctgct ctttcactgc
      481 ggggcgaaca tcagaaactt ctactactgc accgagtcgt ttcgcagtgg cgattgcctg
      541 taagccagct acacctgctc caatgatcac caccttcgca ggttgtacgg ttcccgcggc
      601 agtcatcatc aaaggaaaaa ttttagtcag agaattggca gccatcagca ctgctttgta
      661 tcccgcgagg tttgtctgcg aactaagagc atccattcgt tgagcacgtg ttatgcgagg
      721 cactgcatcc atggacagca cattcacttt gttagtcgct gccagtttca caaggtcggc
      781 gttgctcagt gaatagaaga atccaacgta aagtgcgcct gctttcagca tttcaagttc
      841 gttcttgccc gaagcaggat gttgaattgg cttttgaact ttgaccagaa cgtctgcctg
      901 gctatacaaa tcgccagctg tcgatatgat ttcagcgccg gctgccttgt agtcttcgtc
      961 agaaaaacca gccgcggccc ccgcgtcttt ttccacacga acctgaaatc cgctggcgat
     1021 gaattttttg actgattctg gaacgacggc gacacgcttt tcgcctgcta cgatttcttt
     1081 tggaattgaa agattcactt catcctctcc gtaaacggtg aaaggacaaa tgagcccgca
     1141 ttccaacggg gtcaaatcaa aagagcacga atgctttttc ggtcaaacgc tgaaaccaga
     1201 tccacggcat gcgcgctgtg ccaaagtgcc cccatgtacc acccgccgaa ttctgaatga
     1261 gaacttcctg ccgcgttatg tcgtatccga tgactccaac tgtgtgaatc gcaacaaact
     1321 tgccagctgg agtccagagc ggctcgcgaa gatcgacaaa gttttccgtg atcttcatgg
     1381 atacgatcaa gacagaaccc tgcagcaagt gtgcgtgaac ctggtcaaag tcacgaaagt
     1441 cgattctgga ataaccgcga acgcgaaatg cgtccgcgtt ctgaagcgca tcggggccag
     1501 gcttgcggaa gaaatccttt tcattgaagg gcatgagttg ctctgtcggg catcctcgcg
     1561 catgtaaaaa gtctagagca tcgagtactg agatgccctc gtcttttccg ccgttgaggg
     1621 agttgtatac aaaggaagga gagcacgtgt atccaggctt gccgcgccga cgatggtagt
     1681 agctcgtcgc cagataccct gtggagaagg caacaccgct cgcctggcgc atctggttgc
     1741 ctgcgggagg caaatcagat agaataaaga cctgcggcac gacatctggc aaaggagcca
     1801 gcatgatggg cacagctctt tcttcgtctg tgtagttggg ctgaatctga aaatcatccg
     1861 gccgaagcgc ttcgcgagag gagcgagtgc cagggcgaaa gcgggccgct tgttcttctg
     1921 tgatgcagga tccggtaaga atcagagcgc ccaggagaaa gatctgcgat tttgacggaa
     1981 tacttcgtag catgcgattg caacagagtt gctcaggttt aaagatcggc acttttctcc
     2041 taccgggata cgaagtaagc gaccatgctt tcttacatgt tcagtgattt cttgcggcaa
     2101 tcctgcagtt tcttgaccaa aaaggagtgc ttcctggccg gaaaacgtga agtcccacat
     2161 agaatccgtg gcaaagcgcg aaaacgctac gaggggcact ccaatcgatg ccagactcgc
     2221 atagaattcc tcgtagtccg catgcctttt gaggtccagt tgagcccagt agtccagtcc
     2281 tgcccgccgc accgcctttt cgtccatgga gaaggaaggc tcgcccacta tgtgcagcgg
     2341 cgtgtccgtg cacactgcca gtcgcgcaat gttgccggtg ttcgggggga tctccggtct
     2401 atataaagcg atgtgcattc cactcggaaa ccatttgcct tgaggggtgc acgccaaaat
     2461 tgggattaga actatgaact attcagcgca gagacttaat atagtagaac cctctccaac
     2521 cctggctatt tcagccaagg cggcagaact caaagctgcc ggtagagacg tgatcggctt
     2581 tggtgcgggt gaacctgact ttgacacgcc tgatcatatc aaagccgcag cacaaaaggc
     2641 tatggcggag ggtcagacca agtatactcc agtgggcgga actgcgaagc tcaagaaagc
     2701 ggtggtagac aaattcaaaa gagagaacgg tctcagttac gaacccaaag aagtcatcgt
     2761 ttctaccgga ggaaagcaag tactgttcaa tctattcctc gcctatctga atccgggcga
     2821 agaagtgatt gtacccgcgc cgtactgggt ctcgtatgct gacatagtca gaattgttgg
     2881 cggagttcca gtcatcgttc agtgtcctgc atcgcagaac tatttgattc aaccttcgca
     2941 gattgaagag gcggtcactc cgcgcactcg tgcgatcctt ctttgctctc cttcgaatcc
     3001 caccggaagc gtgtattcag aagccgagct tcgcgcgatc gccgctgttc tagaaaagta
     3061 tccggacatt cttatcattt cagatgatat atacgaacac atattgtttg ataacagaaa
     3121 gtttacaaat ctcgccatgc tatttcctga gtggaaagat cgcatcttca tagccaatgg
     3181 agtttctaag tcatacagca tgaccggatg gcgaattggc tacggtgctg gaaaccgtgc
     3241 actcattgaa ggcatggaga cgatgcaatc tcagagcaca tcgaacccga gctccatatc
     3301 tcaggcagcc gcactcgcag cactcgaagg gccacaggag tgcgtgtcca tcatgcgcgt
     3361 ggctttcgaa aaaagaaaaa acattgtgat gcagaagttt tcgcaagttc ccgaaatcac
     3421 tctgtctgca ccaggaggtg ccttctacgt gtttccaaac ctggatcgcg tatttgcgct
     3481 gcctggattt caaaaacttc gcgcagcatc gaaagacaga tctctttcgc gcattttgtc
     3541 tgcgcatctg cttgaaaaat atgacgtggc agtggtcccg ggaattgcct ttggtgacga
     3601 tagcggaatg agattgagct ttgcactcgg agaggatgct ctcataaagg gcctggaccg
     3661 aattgccaaa atggtagccg acttgcgatc atgaaacctc tgttaggtga tgttcgtgag
     3721 tggctcaaag aacttgatct tcctgagccc acgaacattc tacctttgca gaaggatctc
     3781 tccactcgca catactacag gctgactcta aatgaagtat gtgaattggg taactctcta
     3841 gttttatgcg tccattccgg tcaatacgaa gccactattt ctgattttgt aagcattagc
     3901 agacagttca aagaagccgg tgtggccgcg cctgacgtga tcgcgcaaaa gcagcactgg
     3961 ctcctccaaa cagatgccgg ttccatagat ttgcgtcagc attgtctgga atggatcgaa
     4021 aagggagata cgcagtcgat tgaaataaca tatgagcgtc tgctggatca acttgtgcgg
     4081 ttgcaggcgg tgactccgtc tcacccggta tctggtcggt tctttgattt tgataaattg
     4141 cattttgaga tggaacattt tgctctccac gtcgcacagg cggggcagaa atttgcacgc
     4201 gagtactttt acagttttga atttcaaatg tttctaaaga ctatatgtga gaagcttgcg
     4261 caatcatcat ccaaggtttt cgtccatcga gatctccaca gcaagaacgt tatgcgctct
     4321 tctgaaaacc tggtatggat tgattttcag gacgcgcgaa gcggactgag atactatgat
     4381 ctggcaagtt tgctctacga tccctacctg cctctgtctc gtgacttccg gaccaggatg
     4441 ttgtccagct atgcaaaaaa gtccgggaaa gccgtggagg aaaacctgtt ttacatgcaa
     4501 tcatttcagc gcctggtaaa ggcgatcggc acttacatgt ccgtgattca cacgcgccag
     4561 gacagggact atcttggatt tctacgtcag gcggcgattc atctggaaga agtggttcag
     4621 ctcggcggat ttccggacca tacatttttg ttcgtccgcg gaatgcagaa gttttcagag
     4681 ttagaattct gatgaacgca ttcttgccag cagccggttt tggcactcgc atgggagacc
     4741 tgacgcgttt cattccaaaa cccctcttgc ctgtgggcgg tgtcccgatg atttactacg
     4801 ctctgttcca actctggaga tggaaagtgg accgcgtcgt tgtgaatttg cattttttaa
     4861 aggaaaagat tgagcgtgaa ctctcaggat tcaaggccat ggaaattatt ttttcgcgtg
     4921 aggatcccat ccttggtacc gcgggcggga tccgcaatgc tctaccactg ttttcgtctg
     4981 acgcgatcgt ggttttgaat ccggacagca ttgtgtttac cgatcgaagt ctcgagcaaa
     5041 tcacgtctgc tgcgtcctcg tcgtgggata gcttgcttgg aattgctccc ctgcctcagg
     5101 gttcagccaa cactcctctg tcctggaagg aaggtcagct tagtttcgag gaaagcggtt
     5161 cctactacta catgggtgcg tccgtgctca agctgtcaca atttgatcat gttgcctccg
     5221 gttacagtga gctgggttct ctgtggagag agcaggcgta cgaaggcaga ctggagggtt
     5281 tcctgtatga aggaaacctg ttggacgccg gcaacgcatc tgaatatcgt tcccttccag
     5341 agcaaattgt cccagggcat ctggccgcat catggaaaga attcataaaa aattgcaaat
     5401 aattctgctc cttgtgtttg cagtgccgtt gtttgcggaa acgcccattc aaaatgtttc
     5461 tccggcaacg ccaactactc ccgccaaacc ggactcgcga ccctcaaagg aagctcgcac
     5521 ccgtgcagaa acggacttag aactcaagag attgactgcg tccctggatg ctgcttttag
     5581 actgcaaaag aagtttgaaa ttcctggttt actggaagac ctgaaaaagc tcgcgccgga
     5641 ctccgcgcaa aacttctatt tccaggctct ggacgcgtac actcgcggtg agaaactgaa
     5701 ggctctggct ctcatcaaga aatcgatttc aaaggatccc ctcttgaccc cggcctatac
     5761 cataggtgga ctcatctatg cagacggagg caaggatcaa tcggctctcc cgtttttcga
     5821 gcgtgcggtc caacaaagtc cttacgatcc gatctacctg ctcaatctgg cgctctgcaa
     5881 gaatcgtctt ggaatgaaag ccgatgctct ctcgttgcta gaccaggctt tgcgtgctcg
     5941 cgcgaatcta gcggaggcca tgtattggaa aggtgtcctt ctcgcggaaa acggtcagaa
     6001 cgcggaagcg caaaaatatg cacgcggtgc tgttgatgcc ggtttgaaga atgtttctgc
     6061 gtatcgcctg tggctgcagc ttgcgtttga ggcggcggac gatgtagacg tggcgcgcgc
     6121 agtgtcggag ctttcgcagg acgcgcgaga atcagcacgc tggagactcg cagccgaata
     6181 ttcgtttcga ttcggggatt ttcgcaaagc ggtttccttg ctggatcgag ccttccagat
     6241 agacgaagga atgccggagg atcgccgcat gtatgttaga tgtctatttg cagaaggtca
     6301 ggatccaatg ccctggattc gatctcagcg gctgagcgat gcagaacgtc aggaactgcg
     6361 gacgctcgtt gatttacttc agaatactcc cggtatagtt cagagtagag atcccctggt
     6421 tcgtcccgct aaataattat cgcggttgtt gccacgttag tcgacattga tcctgatccc
     6481 atcctcttcc tctataatgc gagaagacat gcttggattg atctttgtag actgagtcca
     6541 tagatccgga aaaaaggaat ggagttctgt tgtgtgaaga gggcgtggcg catggccgct
     6601 ttctctatag tggcccgtga ttggaatcac acggagtccc cgcactccag gctttttagc
     6661 ttcaaattcc atgagtacaa tcatgttgtc tgtttggaat ggattgctac ttccaaacaa
     6721 aaaatttccc agtgaatagc agataacggt atttttggaa gtttcaattc cctgtgcgac
     6781 gtgaggatgg tgcccaatca cagcagacgc ccccgcctgg atgaattgtt gagccagtgg
     6841 gcgctggtcc ggcattggat atggattgta ctccattccc cagtggagac ttacaatgac
     6901 gtggtccgcc agagttcgag cagcttgaat ttgcgctaga atttgctccg gctttgcaat
     6961 gccgggtgtt ttgtcgtctg cccacatacg ctcatcgccg atggctgatg cggcaactac
     7021 ggcgaatcga atgtccccga ttcgaaagag cactggaaga gacgcttcct ttgcatttgc
     7081 tcccaccccc gccgtcagaa ttcctgcctt tcgcaggttc tgtctcgtat cttccaatcc
     7141 ttcgggcccc atatccatgg catgattgtt ggcaagtatg gccagattca gcttgagcgg
     7201 agtcagtgtt ttgatcgaac ccggacttgc gcgaaatatg tgggaccgac ctgacgcagg
     7261 ttctcctttg tccgtgatag cggtttccac attcagaatc cgaaaatctg ccttttcgaa
     7321 agtgcgttgc atttcgcgca caggcgcgag agcatcctcc ttttcaatca gttcctcgat
     7381 gccccattga aagtgtgtat ctcctccaaa aaggatcgtc gctttgccag ggttcgcggc
     7441 ggggtaagat ggtattgcag tctcttccgc ggatccgggg ttcggaagaa gataccagag
     7501 agcgcaggtt gcaacaaagc agaccagaac taagcgcgct ttcacaattg caaaagatct
     7561 acgcgccacg ctccgcgcct aacaaaattg ttcgcacaac aaaatttcgt ttcgtcggaa
     7621 tcatagtaga tttcacaagc gcgagggaaa tgcagggaca tgcgcaaagt tatttgcatc
     7681 gccctcattg cttccctgac tggttgtttc aacaagatcg ttctggcacc ctttggcgcc
     7741 agtggcgatg acctggagct ccgagagcat gttttatacg gacgtgcatc gaccgcctgt
     7801 ttttccggtt tctccagcag gaccaaggtt ctcatcgtgc ctgttcaagg tgtcataggc
     7861 gcggacagca tgctttcccg gggcggtacg tctgttgcgg ggatcaagag aactctggaa
     7921 atcgctgaac aggatcccga aattgcggct ttgattttgc tgattgattc acccggcgga
     7981 accgtgtccg cttcagatca aattcatcgg cttctcatag attttagtgc gaaaacaaag
     8041 aagcccatct atgcgcacgt agatggcgtg ggcgcgtccg gggcgtacta tatagcctcc
     8101 tccgccctgg agatcaacgc gtcttctgct tccatagtgg gaagcattgg agtgatattg
     8161 cagagtttta gtgtgcaagg gctgatgcaa aagatcggca ttgagtaccg cagtctgaaa
     8221 acaggaaaga acaaggatag cctcagtccc tttagagagc tgcgagaaga tgagaaagac
     8281 ttcttcatga agcagctgac acaggcgtat gaaatttttc tgcgacggat catggatgga
     8341 agaaaagaca gaatttcgga agacaagctc agacagctgg cagatggatc cgtttactca
     8401 gctgcggatg caaagcagta tgggttgatc gattccgttt cgtatataga agattacctg
     8461 gctgatgtat ctaagagaat ggatctacat gatccattgt tcgttgctta tctgccgcca
     8521 ggaaaaagct acagcctgta caatgttcga tctgatcggc gtgcgagctg gatgagtttg
     8581 cttgagatgc cagaccatcc cacggcagcg atgtactacc tgtgggacgg tggcttttag
     8641 gtgagaactt agaaagtctt gttcggttcg atcttcagcg gatctttctt cgtatcgcgt
     8701 ttgattgtgt tctcgtctgc gcgacgaaca cgttcgcctt tctctggacg agctgtttca
     8761 tctttgagtt gagcaatctt gtcatctgtg ctgccgttga tttcaacacc tttgattgca
     8821 tcgctggact tgatttcatc aagcagtgaa ttgtattcca tgatcgtatt gcgctggaac
     8881 actgctagtt tctttaatgc cgcgagcgac tctttagaaa gtggacgcac tgccttgtct
     8941 gaaatgtcct ggccgctgcg aatggaaagt tcccctggtt ctgagagaac cacttccttt
     9001 cctttgtcgt ttttcaccgc aactgcaccg tcatagagtg caactcgtga ttcagtcggg
     9061 ctatccacag aaaccataaa tgctgttccg cgaacacccg ccactgccgt aggagattga
     9121 actgtaaatt cccccgtgcg atccatcttg cgaaggaaaa acgcagaaga tcctgacttc
     9181 aggtcgatct ttgtcttcag gttttcctga agagacgcca gggtcagttc agtgctttcg
     9241 ctgagtctta cgatcccctg gcccttgatg aagatctcag ccgtggatgc agccgcagtg
     9301 gacacacggt ccccctgctg gagcatatct cccaccttga gttgctgatc agcttcgccg
     9361 cggtgtagag ttacttctcc cgtgatgaat gtagtgcgag ctacagttga ttcggattct
     9421 tttacttgcg atccctgctt gcaggccgcc agtcccagca caagcgccag ggcgattaga
     9481 gtgtgtttca taatagttct ccctttcaat aacgtaatag tgcgctggaa ttgcggccag
     9541 caatcaaaat ttattgctat tcctgggata gtaccggtcc atggaccccg ccatgctggc
     9601 ccgggatacc gaagaaatcc agaggatcga gggcttcaaa agaggctacc tgcctgttct
     9661 agaggctcaa agcgcccacg cgcgggcggg acagcaactg ctcacatcca atgctctgac
     9721 gccggaagag aagaaagttc tgtctcaggc gctcccagag ctcgacaaat tgctttcctc
     9781 tcttcagacc ttgacccgcc agatgactat ggatctcaat ccgaataaca tcgcgaagga
     9841 ggctgctcga gactatcacg cattcttcaa gggcgatgtc atggatcacc agatgtcatt
     9901 catcgtcttt tttaagacgt acatgcatct aacccgcttt ctcaagtccc tgtccgattc
     9961 atggaagcga atccgagatt tgatcacaga atctaaactt gctgacaatt ctgagtttag
    10021 aaagcagttg ctgcacttcc gagtcgggcc ggtcctggca gacattgaag ctttgcgtct
    10081 gttctgcatg cgcatggcca cgatccttca gatcactcga gatcccctga catcgcgcga
    10141 cgttgcgagc acaggaaagt atcgtgagga cattgcctat cagtggtcca cagtatttac
    10201 cgagaatctg gaagcgctca tccatcccac cggtcctgac tgggattcca tgggaatcgc
    10261 cacagctcgc gcggttacga atgcacctcc gcaggcgcct cctgttggag ccgccgctta
    10321 tcgtctgaat gcgggtggca cccagagctg gaacaattct gcgtattact actttaccta
    10381 tgatccgaag ctcatggagg gagagagagc gcgccttacg aatattgtgc acattgacac
    10441 tcacatgggt gcggatcaga acgttatgcg atcagagagg attctgaagt atacgcgcaa
    10501 agtggctcat ccggacgtgc acgaaaacgc gaacgtggag ttggagtaca ctggttttct
    10561 tcacagcttc tttaatctcg taatcgaaat cagtaccctc aatatgggca ttccgcaaaa
    10621 aactcgagat ctgtttttgt ttcacctcgg gccccagtca ttctattatc tcactcgtcg
    10681 ttttcttcag gagcttgata cagggaccat tcaccgcaag tccggacgcg gaacaagcat
    10741 ggagaagttt gttccaggtg aattgctaaa aaagatcatc ctcgaatggt ggcaggcgca
    10801 tatcctcaac gtagttggaa tggagcgaga cgatctttct cagtacagaa tgatttgcaa
    10861 attagtaaaa gatacttatg atcgtctgtc tgcaaaagca atggctgaat atgatcagtt
    10921 gcccgagaac gaaaaggtcg gtaagaatcg cgcagaaatc attcgcgaaa acctgaacaa
    10981 gtggttgggc ccaacgaata tcattgtgtt ccgccgtttt ttgaaatcgg gtactcctta
    11041 atacaaagga gaggcacagt ccactgcttg cctggattga ctacactacc cactgtctga
    11101 gcccgcgcaa atagcaagtt ttcaccaact ggatc
//
LOCUS       DAORFF010000036        11008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2687_length_11008_cov_3.803433, whole genome shotgun sequence.
ACCESSION   DAORFF010000036 DAORFF010000000
VERSION     DAORFF010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11008
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2687_length_11008_cov_3.803433"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PKK76_02675"
     CDS             complement(<1..334)
                     /locus_tag="PKK76_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67396.1"
                     /translation="MTRKEMLLASGAAIAAYKTAGLLADPKDNRGTGIPTLDLSLAAS
                     VCVANGERCVDHCLDLLRNGDDSMGPCSKAAHDMVAICRAMVTLAASKSPMLKKYIVI
                     CKEACETCA"
     gene            complement(331..954)
                     /locus_tag="PKK76_02680"
     CDS             complement(331..954)
                     /locus_tag="PKK76_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNL67397.1"
                     /translation="MSCREPDRATEDEHTIMLRIEKTFVRLICILVGMMAVHCRTPRF
                     EVLEPPVSLEGISLVDVDHRVVDTKDILFVVTGYTRCPDICPTTLRSAVMVMDRLPAA
                     SRARFAFLSVDAKNDSPQKTRDYASGFSKRLIALDPSIESSRRFLTLASVFASVDSET
                     ALIQHSTSILWVQDGKIMRKIPVRFASQDLVEEIQDAEREKAERRNM"
     gene            1041..1364
                     /locus_tag="PKK76_02685"
     CDS             1041..1364
                     /locus_tag="PKK76_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67398.1"
                     /translation="MRKITIMFTCVALIIFADCATVQNFGEPRRDPAYVFAGTRANLN
                     DLGNTSDRVDYCFAKLDFPFSFVLDIILLPVAIPLALTWHEKPDPDEDTSPNQAPAKD
                     EADKK"
     gene            1501..1824
                     /locus_tag="PKK76_02690"
     CDS             1501..1824
                     /locus_tag="PKK76_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67399.1"
                     /translation="MGRNRITSVFLSAALLASVAVPSLHALTFDSGKESSREILCGGH
                     SLQIPARILKGTSETFARPGHLILATPSPTLTGHNLVSYTVPGSEIFLKALAHAYHFA
                     RGPPV"
     gene            1864..2670
                     /locus_tag="PKK76_02695"
     CDS             1864..2670
                     /locus_tag="PKK76_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67400.1"
                     /translation="MYYLFIKRAMLAPAFLYLASCGGILSQASEDTSTNALIALAALA
                     GSSCASYSEPVISNSLFSGGTRTLYNVSGCDTAGWSRIGFTSDGTTQVSDASKVVTDS
                     SYSGFGTNIEVTFTVTSASGTLDVMTYGTGTFPGTSTTNPTVRIVAGSSCSAQARHSG
                     TGALNNMSTCTDTTTLAVGSTYTYCIDFNRGTGSSGSYTGFGNMLAVWSRACDQVASS
                     ERDQMADVKLMQMMNIPTMNPNNSGKVGFSSNGISLKSFKIGNTIYSSMM"
     gene            2678..3286
                     /locus_tag="PKK76_02700"
     CDS             2678..3286
                     /locus_tag="PKK76_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNL67401.1"
                     /translation="MKPSTPWLAGFRQLRIALFAAAIAGSLTLLPANLQSEIPLPPGG
                     DFTLQSEQGRVSLSDLKGQVVVLYFGYTSCPSVCPVSLSMISSAFRGLTPEEQASVRI
                     VFVSVDPERDSPKIVGNYARAFGKTAIGLTGSPEQIRETAKRYGVYYRKANLDSAMGY
                     SVDHSSVTYVINARGRLLRLLKHGTKPSEIQEAMRDAIRSKE"
     gene            3283..4779
                     /locus_tag="PKK76_02705"
     CDS             3283..4779
                     /locus_tag="PKK76_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNL67402.1"
                     /translation="MKHYIFFTICAILPLSLFAQNASFQNPYVQLVPEGMKNSAGFVV
                     VKNTLDTPLKIVRASSPRAASVELHTHTMENGVMRMRPVQVIEVPAKGETSFDPNGYH
                     FMLIGLNGKLVEGEKIPITVEFESGESQTIDFTVKPMATPAMDHSQHSGHNHAGHNHN
                     VADMVAPAGVMGAHMHHPGAWMIDYRAMAMQMNGLQNGTRGVLPFEVLYGIYQDPRVQ
                     MPMTGLAPPSPLLPAARIEQNQFRYMSTGTEMSMEMHMISAMYQAGSDTMLMVMVPYQ
                     ISRMKMLANNFQTANMQARGVGDVSLSATFRVWTSDAHSVFLGAGIALPTGSIDEKDW
                     MPQMGKSQVAYAMQPGAGTASFLPQVAYTGREDRLSWGTQFDGTMRAGKNDNNYRAGN
                     RYNGTAWASWRFADWISVSARVLQQKWENYSGQDPSLDPMMDPGNDPRLQGGRRTDVL
                     AGVNLVATGGAFSGLRLLLEFGRPVRQHLNGPQMSTKWTGNFATQFSF"
     gene            complement(4793..6517)
                     /locus_tag="PKK76_02710"
     CDS             complement(4793..6517)
                     /locus_tag="PKK76_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL67403.1"
                     /translation="MFQRVFAIGISSHFPEKLNEKIRLSNKFAIFLAISALPYYPMFY
                     QLKIPYTMELLTFCIVGYCATLVFNALKWNAFARVYMISFFNLMLYAYAMMFQVRTGL
                     KLCYFATATFSFSLFRSVEWRLLVVSLIGSAFAFVAVEFGIMPGWISAIDLGDPEHPL
                     NHAIALTALSLTIGSSLLYFAEVRNYEKKLFAASEQKSLFLSTISHEIRTPLNVIIGM
                     TGLLGDRGLSNQEKDSYVGSIQKSSEYLLSLVNDVLDFERIEKGKVSLDLKAANIKDL
                     FATVKNIMTPLAREKNLSLTVSYPEQAGTYYVFDGRRLSQILINFLANAIKFTGSGGV
                     ALDVTVDKEERDFCVLRFRVVDSGSGISREDQARILEPFFQVSGSSSIQPGFGLGLSI
                     ARELLRLYGAEISVQSEPGKGSIFAFSLRLEKATGASDERNREEHRLPGLHVLAADDS
                     ELNLLVLQKMLGLWDVQVTQVVNGRELVSTYAADSAKYDCVLVDSHMPLMGGVEAAHE
                     LKNRNPDLPVYLVSADATMELERAALAAGANGVITKPINVSTLYNVMLQVQKKKGKIP
                     SPLERSMH"
     gene            complement(6713..7486)
                     /locus_tag="PKK76_02715"
     CDS             complement(6713..7486)
                     /locus_tag="PKK76_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67404.1"
                     /translation="MDTSRSIERVGGFCCSVLVALYVIAGVTFLLLPVKASVLDPVGF
                     ANAYTTHRFVYRAFFLSFGFIAVFTFGAIPAISALVRSESEGLIRWSSNLAYLGSGVL
                     ALNSFRTVGLIDMRTEIFLSLDAEGQKILAAAPLVMDQHAYLTFGATGFYFLTVGILL
                     FKSEGGLLASVGVMLGVVFELVVIGWAAASMPLLSGLATVGGVLIGPVWFFLLGQRLR
                     DAGKVTASVPPAKPVRAHAHREKVKKKRNSADDDLLDDI"
     gene            complement(7589..8644)
                     /locus_tag="PKK76_02720"
     CDS             complement(7589..8644)
                     /locus_tag="PKK76_02720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNL67405.1"
                     /translation="MRRVIALIAIPALSLSGAAPIGANLHRIPGSAVFLTFDAHPCKD
                     TMLHKCEDLPAIDGAWKLLDTLRANGIRSTFFFTGQFIEKYPDIVRRALQDGHEIGSH
                     LNSHTHPLEFQEKHITLNKTWFMNELLSADRALVKTGNGHAVKFFRMPYGLASYSVLP
                     GARRNILQWAQEAGYTHVDWSVDTLDWISRDARSHGSFLPPYLTSRQMEERVIADRGG
                     AIVLSHLTRYRPFGEPSVNEMIPDLLVRLKKQNLRTARISDGLGVVPPAELAVVKEES
                     VREVQKETPVAAVNKCNYSWDFKRVHICITGMTESDCAGLEESFLSREEECICTKPGV
                     KYNLQNLGELVQISCKL"
     gene            complement(8665..9888)
                     /locus_tag="PKK76_02725"
     CDS             complement(8665..9888)
                     /locus_tag="PKK76_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67406.1"
                     /translation="MKVPKRLIASLAVAIVLIGVIAYFLLAPSRAQREQAQRESGSQT
                     EPGLRTDENGMPVIDDDPAAHLERYRKWAQYPPNSRPLFAGQVDLLDPYNAEKNPVYV
                     VQTPAEGCSANAQGLLECAKKAVFSDVECKLTPERYISIGTKDFHLYLSCTSKKQNEG
                     KPLAIESIETKFYRQLFKNIIPSLPPIAVGDDGANGDEKAGDHIYTITVRPTSQDWGD
                     MFLEAEFKVAGFKHVQRADFFSTPHKTAEFQSGIRDANRNGQLVVSVPVNVFKAGYYK
                     FDANLVQKEDDHQPVASASWEGDLTPGAQTIDIQFFGKVIKDQKLNGPYVVKNLRGMR
                     NNSPVTPSMLKNAIASGKPIGPQQHKEPLQEYMEPYAGDYSTQAYRADDFSSDEWQSE
                     EKDRRIKYLESLGTN"
     gene            complement(9927..10832)
                     /locus_tag="PKK76_02730"
     CDS             complement(9927..10832)
                     /locus_tag="PKK76_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67407.1"
                     /translation="MTIAADMNLFFARLIALLIMTPFFSCAVLFWKDASNSSGGTWKG
                     NDRFIEIQTEFQRKSSWYPLNQNALTRNYSTTLLLHKIAGESSTSSEKIAHYSGWTLN
                     DTLFLAGSSLIAIRGTSNSLGDENRELLSVSLKEGTVSDASVILKPDAFLLAAVPSPD
                     GKWIAAFLTDATNEKRTGNLFVDFYEFRGSSSLQKKSRVTVQWSGVPGNPELSWSRDS
                     QGLYLHVESAVLYLTAGGSTARNADRFPACFIPTSSGRNISESGLFFGRSQEGQVEIR
                     KTNEGIRFDAIPMVNNINDLGKGCP"
BASE COUNT         2839 a         2923 c         2639 g         2607 t
ORIGIN      
        1 ccgcacacgt ttcgcaggct tctttgcaaa ttactatata tttctttagc atcggcgatt
       61 tgctcgccgc cagagtcacc attgcacggc aaatagcgac catgtcgtgc gccgccttgg
      121 aacagggtcc catgctatca tcgccgttgc gaagcaaatc cagacaatga tccacacatc
      181 tctctccgtt cgcaacgcac accgacgcgg ccagggaaag atcgagcgtt ggaatcccgg
      241 ttcccctgtt gtcttttgga tcagcgagaa gtcccgccgt cttgtaggca gcaatggcag
      301 caccgctcgc cagaagcatt tcctttctag tcacatgttt ctcctttctg ctttttctct
      361 ttcagcgtcc tggatctcct ctaccaggtc ctgtgatgca aacctgaccg ggattttgcg
      421 catgatcttt ccatcctgta cccataagat ggaagtgctg tgctgaatga gtgcagtctc
      481 tgaatcaacg cttgcaaata cagacgccag agtaaggaag cgacgcgatg attcgatgga
      541 tggatcgagt gcaattaacc ttttgctaaa gccagaagcg tagtcccggg ttttttgcgg
      601 cgaatcattc ttcgcgtcca cggaaagaaa tgcgaacctc gcgcgacttg cggcaggcaa
      661 tcgatccatc accatgacag cagagcggag tgtagtggga catatgtccg ggcagcgcgt
      721 atagccagtc actacaaaca ggatgtcctt tgtatccaca acgcgatgat ccacatccac
      781 gagcgaaata ccttcgagag aaactggcgg ttccagtact tcaaagcgag gcgtcctaca
      841 atggaccgcc atcatcccca ccagaataca tataaggcgg acaaaagtct tttctattcg
      901 cagcatgatg gtgtgctcgt cttctgtcgc acgatccggc tcccgacagg acatggttca
      961 tcgaccggtg aagtttgtcc atcggttcac gattccctga atcttgttgc acaatagtct
     1021 cagcatgaaa gaatccgccc gtgcgtaaaa tcaccatcat gttcacatgc gtcgccctga
     1081 ttatcttcgc agactgtgcg actgtgcaaa actttggaga acctcgcaga gatccggcct
     1141 atgtattcgc aggaacgcgc gcgaacttga atgatcttgg aaacacgtcc gatcgtgtgg
     1201 actattgttt tgcaaagctc gactttccct tcagtttcgt cctcgacatc attctgctac
     1261 ccgttgccat tccgctggct ttgacctggc atgaaaaacc agatcccgat gaagacacat
     1321 cccccaatca agcgcctgcc aaagatgagg ctgataaaaa gtgaattcca ctcctgtaat
     1381 tgatctacgt taggcgatcg cattaaacag aaagcagccc tatgacaaaa atcaggccag
     1441 caagaaacgc cccacgagct cttgcacttg actgtctcct atccagaaga aaagtgagtc
     1501 atgggcagaa acagaatcac atctgttttc ctttccgcgg cacttctcgc aagcgtcgcc
     1561 gtgccctcgc tgcacgcgct gacctttgat tctggcaaag aatcctcgcg agaaatactg
     1621 tgtggtggcc attctcttca gattccagcc agaatcctga agggcacatc cgaaacattt
     1681 gctcgccccg ggcatctcat tttagcaacg ccttcgccca ctctaacggg ccacaatctt
     1741 gtatcttaca ccgtccctgg cagtgaaata ttcttaaagg cccttgctca cgcctaccac
     1801 ttcgctcgcg gccctccggt ctagatcgtt cgcataacga tcgttcgatt ctggaggaat
     1861 catatgtatt acttatttat taagcgagcc atgctcgcgc cggccttcct gtatcttgcc
     1921 agctgcggag gaatcctttc tcaggcatcc gaagatactt ctaccaacgc actgatcgcg
     1981 ctcgcagcac tcgcaggctc aagctgtgcc agctatagcg aacccgtgat atcaaacagc
     2041 ctgttcagcg gcggcacgcg gactctttac aatgtaagcg gatgcgacac agcgggatgg
     2101 tctcgcattg gctttacatc cgatggaacg actcaggttt cagacgcaag caaggtcgta
     2161 acagattcca gctactcagg ctttggcacg aacatcgaag tgactttcac ggtcacctca
     2221 gcgtccggga ctctcgacgt aatgacctac gggacaggca catttcccgg cacatccact
     2281 acaaatccaa cggttcgaat cgtcgcaggt tcatcttgtt ctgcgcaagc acgccacagc
     2341 ggaacgggtg cactcaacaa tatgagcact tgcacggata cgacgacact ggccgtgggc
     2401 tctacgtata cgtactgtat agacttcaac cggggaacag gcagctccgg atcctacact
     2461 ggatttggca acatgctggc tgtctggtcc agagcctgtg atcaagtcgc ttccagcgaa
     2521 cgagaccaga tggcggatgt gaagctcatg cagatgatga acatcccaac catgaatccg
     2581 aataacagcg gtaaggtggg attcagctcg aacggcattt cattgaaatc gtttaagatc
     2641 ggcaacacga tctattcgag catgatgtag gcttgagatg aaaccatcaa ccccctggct
     2701 ggcgggattc cgccagcttc gcatcgcgct tttcgccgca gcaattgcgg gttcactcac
     2761 tctgttgccg gcaaacctgc aatcagagat tcctctgcct cccggcggag atttcactct
     2821 gcaatcggag cagggtagag tctccctgtc agatctcaaa ggccaggtgg tcgtgcttta
     2881 ttttggatac acatcctgcc ccagcgtctg cccggtttcg ctttcgatga tctcgtccgc
     2941 atttcgcggg ctcactccgg aagaacaagc aagcgtaaga attgtatttg tatccgtaga
     3001 tccagaaaga gacagcccga aaattgtagg caactacgct cgcgcttttg gcaaaactgc
     3061 gattggactg acgggcagcc ccgagcaaat acgagaaact gcgaaacgat acggcgtcta
     3121 ctatcgcaaa gcgaatttgg acagcgccat gggctattcc gtagatcatt catcggtcac
     3181 ttatgtgatc aacgcaaggg gtcggctgct cagacttcta aaacacggca caaaaccatc
     3241 cgaaatccag gaagcaatgc gagatgccat caggagcaaa gaatgaaaca ctatattttt
     3301 tttacgatat gcgccatatt gccactgtcc ttgttcgccc agaacgcttc ctttcagaat
     3361 ccgtatgtgc agctcgtgcc ggaaggaatg aagaactccg cgggctttgt tgtggtgaaa
     3421 aatacactcg atactccatt aaaaattgtg cgggcatctt cgcccagggc cgcctcggtg
     3481 gaactccaca ctcacaccat ggaaaatgga gttatgcgaa tgagacccgt acaggtgata
     3541 gaagtgccgg caaaaggaga gacttctttt gatccgaatg gatatcattt catgttgatt
     3601 ggtttaaacg gaaagctcgt agaaggagaa aaaattccaa tcacagttga gtttgaatcc
     3661 ggggaaagcc aaacaataga tttcacagtg aaaccaatgg cgacgcctgc catggatcac
     3721 agccagcata gcggccacaa tcatgcaggg cacaaccaca atgtcgcgga catggttgca
     3781 cccgccggag tgatgggcgc acacatgcac catccgggag cgtggatgat cgactatcgg
     3841 gccatggcca tgcaaatgaa cggactccag aatggaacgc gtggcgtgct tccattcgaa
     3901 gtgctgtatg gaatctatca ggatcctcgc gtgcagatgc cgatgacagg tcttgcgccg
     3961 ccatcgcctc tcttacctgc agctcgaatt gaacagaatc agtttcgcta catgtctact
     4021 ggaacagaaa tgtccatgga gatgcacatg atctccgcga tgtaccaggc gggctcagat
     4081 acaatgttga tggttatggt gccttaccag atcagcagaa tgaaaatgct cgcgaacaat
     4141 tttcaaacag caaacatgca agctcgcggt gtcggcgatg tgagcctttc tgctactttt
     4201 cgcgtatgga cgagtgacgc tcactccgtg tttctgggag caggcattgc acttcccacc
     4261 ggttccatag atgaaaagga ttggatgccc cagatgggaa aatcgcaggt cgcatatgcc
     4321 atgcaacctg gagccggcac agcttctttt cttccgcaag tggcttacac tggcagagaa
     4381 gatcggttga gctggggcac tcagtttgac ggcaccatgc gagctggaaa gaacgataac
     4441 aactatcgcg caggcaatcg ctacaacgga actgcatggg catcgtggcg atttgcggac
     4501 tggatcagcg tgtccgctag agtcttgcag caaaaatggg aaaactattc cggtcaggat
     4561 ccttctcttg accccatgat ggatcctgga aacgatcctc gccttcaggg tggaaggcga
     4621 acggacgtac ttgctggagt caatctggtt gccactggag gtgcattttc cggactcaga
     4681 cttctgctgg aatttggaag accagtcagg caacacctga acggacccca gatgtctacg
     4741 aagtggacag gaaatttcgc gacacagttt tccttttgaa ggttcgaatc tcctaatgca
     4801 tgctccgttc aagaggtgac gggatctttc ccttcttctt ttgaacctgg agcatcacgt
     4861 tgtagagcgt actaacgtta atcggctttg tgatcacccc gttcgccccg gcagcgagtg
     4921 ctgctcgctc cagttccata gttgcgtccg cagataccag ataaacaggt agatcagggt
     4981 ttcgattctt cagctcatga gctgcttcta ctccgcccat caaaggcatg tgactatcca
     5041 cgagcacgca atcgtacttt gcgctatccg cggcgtaagt ggaaaccagc tcacgaccat
     5101 ttacaacttg cgtcacttga acatcccaca gccccagcat tttctgcaaa acaagaagat
     5161 tgagttctga atcgtccgcc gcgagcacat gcaagcccgg cagccgatgc tcctcccgat
     5221 tcctttcatc actggcgccg gttgccttct caaggcgcaa agagaacgca aaaatgctac
     5281 ctttgcccgg ctcactttga acgctgatct ccgcgccata caatcgcaat aattctcgcg
     5341 cgatggaaag gcctaaaccg aaccccggtt gaatggaaga gctgccggac acctgaaaaa
     5401 acggctcaag aatgcgcgcc tgatcttctc ttgaaattcc agacccacta tccaccacgc
     5461 gaaacctgag cacgcagaag tcgcgctctt ctttgtctac agtcacatcc agggccacgc
     5521 cgccgctgcc agtgaacttg atcgcattgg caaggaagtt gattagaatc tgactcaggc
     5581 gacgaccgtc aaacacatag taggtgcccg cctgttccgg atatgagacg gtcaaactca
     5641 gattcttctc acgcgccaga ggtgtcatga tattcttgac tgtcgcaaac aagtccttta
     5701 tgttcgcagc cttgagatca agagatactt ttcctttctc aattctttca aaatcaagga
     5761 catcatttac aagggagaga aggtactccg aacttttttg aatggaaccg acatagctat
     5821 ccttctcctg attggaaagt cctcgatcac caagcaagcc ggtcatccca atgatgacgt
     5881 tcagaggagt gcgaatctca tgcgaaatag tagaaaggaa aagagatttt tgttccgaag
     5941 cggcaaaaag cttcttttcg tagttacgaa cttcggcaaa atacaagaga gatgaaccga
     6001 tcgtgaggct cagtgcagtc agggcaatcg cgtgattcaa cggatgctcc ggatccccaa
     6061 ggtcaatcgc agagatccag cccggcatga tgccgaattc caccgccaca aacgcaaacg
     6121 cgcttccgat gagagacacg acaagcaatc tccattcgac acttctaaag agggaaaaag
     6181 aaaacgttgc cgtcgcgaaa taacagagct tcaaccctgt gcgaacctga aacatcatcg
     6241 cgtatgcgta gagcatcaaa ttgaaaaagg atatcatgta gacccgggca aaagcgttcc
     6301 acttgagagc gttaaataca agcgtcgcgc agtagcctac gatgcagaaa gtaagaagct
     6361 ccatcgtgta cgggatctta agctgataga acatggggta gtacggcagt gccgaaatcg
     6421 ccagaaaaat agcgaacttg ttgctaagac gaatcttctc gtttagcttt tcagggaaat
     6481 gcgaagatat gcctatggcg aagacgcgct ggaacataca cactccctat attgcggcag
     6541 gcgcgaatca atcgcggtgg atgaaagcta ctgtacactc tcgttttact ccacagccag
     6601 cggtccttgt cgagtgaatt tggataaatc gcgaacgttc gaagatgaac ggcaccgaga
     6661 ttgagtcgca tttatcgcaa tggatcaacc aatggcgcat aacgtagatt tgtcaaatat
     6721 cgtcgagcaa atcatcgtcc gcagaatttc gtttcttctt caccttttct ctatgcgcgt
     6781 gagcgcgaac aggtttcgcc ggcggcacgg atgctgtcac tttccccgcg tcacggagcc
     6841 tctgaccaag aagaaagaac cacacaggcc cgatcaacac tccaccaact gttgcgagac
     6901 cggagagaag aggcatcgat gctgccgccc agccaatcac taccagctcg aaaactactc
     6961 cgagcatcac gcccacagat gcaagtaaac cgccctctga cttgaaaagt aagatcccaa
     7021 ccgtgagaaa gtaaaagccg gtggcgccaa aggtgagata cgcgtgctga tccatgacaa
     7081 gcggcgctgc cgcgagtatc ttttggcctt ccgcatccag gctcaaaaag atttcagttc
     7141 gcatgtcaat gagacccact gtgcgaaatg aattcaaggc cagcacacca gaaccaagat
     7201 atgcgaggtt cgagctccac cgaatgagac cctcactttc agagcgcacc agtgcggaaa
     7261 tcgcagggat cgcaccaaag gtgaagactg ctatgaaacc gaatgacaaa aagaaagcgc
     7321 ggtatacaaa tctgtgcgtt gtgtacgcat ttgcaaatcc aacggggtcc agaacagaag
     7381 ctttcacagg caagagcagg aaggtcacac ctgcgatgac gtaaagcgcc acgagtactg
     7441 aacaacaaaa accgccgact cgttcaatgc tacgacttgt gtccacaaag cacctcctgg
     7501 cgcccgcgct gctggcaatc gatttgctct tcctgaatgg acgataagcg cctgaatcaa
     7561 cagtgcaact ctttttgact aacttaaatt acagtttgca ggaaatctga accagctcgc
     7621 ccagattctg cagattgtat ttcacacctg gtttggtgca aatgcattct tcttctctag
     7681 agagaaagga ctcttccaga cccgcgcaat ctgattcagt catccctgta atgcatatgt
     7741 gaacacgttt gaaatcccag gaataattgc atttgtttac tgccgcaacc ggcgtttcct
     7801 tctgcacttc tcgcacagat tcttctttta caacagccag ctccgcggga ggaaccacac
     7861 ccaggccatc agaaatcctt gcggtgcgaa ggttctgctt tttcaggcga acgagcaaat
     7921 ctggaatcat ttcattcacg gaaggttcac caaacggcct gtaacgcgtc agatgactga
     7981 gaacaatcgc ccctccccga tccgcaatca cgcgctcttc catttgcctc gatgttaaat
     8041 agggaggtag aaatgatccg tgcgatctgg cgtccctgga gatccaatcc agagtgtcta
     8101 cactccaatc gacgtgcgta tatcctgcct cctgagccca ttgcagtata ttcctgcgcg
     8161 cacccggaag caccgaatat gacgctaaac catatggcat gcgaaagaat ttcactgcgt
     8221 gaccatttcc cgtcttcacc agcgcgcgat ctgcactcaa aagttcattc atgaaccagg
     8281 tcttgtttaa cgttatatgc ttttcctgaa attcaagagg atgagtgtga gaattcagat
     8341 gcgagccaat ttcatgaccg tcttgtaggg cacggcgcac tatgtccgga tacttctcaa
     8401 tgaattggcc cgtgaaaaaa aatgtagaac gaatgccatt tgcgcgcaga gtatcgagca
     8461 acttccacgc gccatcaatg gcaggaaggt cctcgcactt atgaagcatc gtgtccttgc
     8521 acggatgcgc atcaaatgta agaaatacgg cactgccagg aattcgatgc agattggcac
     8581 caatgggagc agcgcccgaa agagaaagcg ccggaattgc aatgagtgca ataacccggc
     8641 gcaatggttt agaaaatcga actatcaatt cgtacccaga ctctccagat atttgatacg
     8701 acgatccttt tcttcagatt gccattcgtc cgaagaaaaa tcatctgcac gatacgcctg
     8761 cgtagaataa tccccggcat aaggctccat gtattcctga agaggttctt tgtgctgctg
     8821 aggaccgatc ggcttaccac tcgcaattgc atttttcagc atagaaggcg taacgggaga
     8881 attgtttcgc attccgcgaa ggttcttcac cacgtaaggt ccgttcaatt tctgatcctt
     8941 gatgactttg ccaaagaatt gaatgtcgat cgtttgcgcg ccaggggtta aatctccttc
     9001 ccaggaggcg ctggcgactg gttgatgatc gtcctccttt tgcaccaggt ttgcatcaaa
     9061 cttgtagtag cctgctttga acacgttgac cggcacggat actacgagct gaccgtttct
     9121 attcgcatcc cgaatgccac tctgaaactc agcagtcttg tgcggagtgg agaagaaatc
     9181 tgcacgctgc acgtgtttga atcctgcgac cttgaattca gcttccagga acatatcgcc
     9241 ccagtcctga gaagtaggac gaactgtgat ggtgtagata tgatcgcctg ctttttcgtc
     9301 tccgtttgcg ccatcatccc ccacggcgat cggcggcaga gatggaatga tgttcttgaa
     9361 caactgacgg tagaactttg tttcaatgga ctcaatcgca agcggcttcc cttcgttttg
     9421 ctttttggaa gtgcacgaaa gatacagatg aaagtctttt gtaccaatag aaatgtaacg
     9481 ctccggagtg agcttgcatt ccacatccga gaacacagcc tttttcgcgc attccagaag
     9541 cccctgtgca tttgcgctgc aaccttctgc tggagtttgc acaacgtaga ctggattctt
     9601 ttctgcgttg tatggatcca ggagatccac ctgacctgca aaaagaggac gagagtttgg
     9661 tggatactgt gcccacttcc tgtatctctc aagatgggct gccggatcgt cgtcgatcac
     9721 aggcattccg ttttcatccg tgcgaagacc tggctctgtt tgcgagccac tttctctttg
     9781 cgcctgctct ctctgcgccc tggacggagc cagcaggaag taggcaatca cgccgatgag
     9841 cacaatggct acggcaagtg acgcaatcaa acgcttgggc actttcatac tttatttccc
     9901 cttttttgaa ctttctcttt cttagatcaa gggcatccct tgcccagatc gtttatatta
     9961 tttaccattg gaatcgcatc gaaacgaatt ccttcgttcg tttttcggat ttccacctgc
    10021 ccttcctggc tgcgcccaaa gaaaagcccg gactctgaaa tattccgtcc ggaagaggtt
    10081 ggaataaaac atgcgggaaa acgatctgca ttcctggcag tcgatccgcc tgctgtcaga
    10141 tacagtactg ccgattccac atgaagatac agtccctggc tgtcgcgaga ccaggaaagc
    10201 tcaggatttc cgggcacgcc ggaccactgc accgtcactc tgcttttctt ctgcaaggaa
    10261 gagcttccgc gaaattcata aaaatccaca aataggtttc cagtgcgttt ttcattggtt
    10321 gcatctgtta gaaacgccgc gatccatttt ccatccggag aaggtacggc agccagaaga
    10381 aatgcatccg gtttcaaaat cacagaagcg tcagaaaccg ttccttcttt gagggacacg
    10441 ctcagaagtt cacggttttc atcccccagt gaattgctgg taccgcgaat cgcaatgaga
    10501 gaagaacctg ccaggaatag agtgtcgttt aacgtccagc cagagtagtg cgcgattttc
    10561 tcagacgatg tagacgattc acctgcgatc ttgtggagca gcagagtggt tgaataattg
    10621 cgagtcagtg cgttctgatt gaggggatac caggatgatt ttctctgaaa ttccgtctga
    10681 atttcaatga aacgatcgtt tcccttccag gttccgccgg aagaattact cgcgtctttc
    10741 cagaagagca ccgcgcagga aaaaaatgga gtcatgatga gtaaagcgat cagtcgtgca
    10801 aaaaacaaat tcatatcagc tgcgattgtc atcgccgagt caagaacggt ccacacaaaa
    10861 aaaagaccgc gccgaattag acgcggtctc ttgtttcgca tggattctaa gttgaatcag
    10921 cgacggttgt agtcagccat tgctgtcttg tgcgtttcgc ctgatccgcc ttgccataca
    10981 gtgtagtatg gagtccagcg agtccagg
//
LOCUS       DAORFF010000037        10965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2702_length_10965_cov_3.854537, whole genome shotgun sequence.
ACCESSION   DAORFF010000037 DAORFF010000000
VERSION     DAORFF010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10965
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2702_length_10965_cov_3.854537"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKK76_02735"
     CDS             complement(<1..199)
                     /locus_tag="PKK76_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67408.1"
                     /translation="MRLLLALLLLCGCHGQIVQAPPSVPISETLSDAYDVGYERERKI
                     RSTDWMSGVNPDVVRTLVGTSE"
     gene            324..1037
                     /locus_tag="PKK76_02740"
     CDS             324..1037
                     /locus_tag="PKK76_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNL67409.1"
                     /translation="MKIPFFERRRRIRRPWTLLFFGIAFLLLPFLNYLGITSQLQISP
                     QMPVVALKLLNPVELILLFTPILVGIGLLAVKRWGWWLFLAYAVVLIVHNIYALARSP
                     GYYNAGALIQTILGVAAVLYFVRRDISAPYMRMYPRGWRIQKRKPVEVEVLVDGIRRS
                     TRDVSDSGLYVAWEDCYREPGEEVHLAFFLDGAEYKCRAGVVRVDEDGAGIAYRGVTR
                     AFKKNIMRDIAKLENLNQP"
     gene            complement(1097..2308)
                     /locus_tag="PKK76_02745"
     CDS             complement(1097..2308)
                     /locus_tag="PKK76_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNL67410.1"
                     /translation="MAIQKIGILTSGGDCGGLNGVIIGAARLALSRGIKAYLIPNGYA
                     GLYNLVDFPGLVELTESRIEDIHVYLAGSEAGNSRVKISKIKDEKKYDRIREGLKKFG
                     IDALVISGGDDSGSVVVDLASQGIPCVHAPKTMDLDLMPYSVGGDSTVARIAEFTRDL
                     YTTGRTHNRIVVMEVFGRYAGHTAFRGGIAAGADAILIPEVPVDFDLLYKHVMGNFIR
                     RVESSDARKGTAAVIVAEGLKDSSGKELVDESSTADAFGHKKLMGAGKYVVKQLEDRV
                     KKDPAVKEFMKRTGQFVEGVYEIPEIREVRPGHLVRCGFTSPVDANFGLEVGAAAVEL
                     HLKGINGVTVISYQDGKIRYMPVAEAIVQRHVDIESIALYERMGICFGRAPANVEPKF
                     EKFSGRPERVY"
     gene            complement(2350..4140)
                     /locus_tag="PKK76_02750"
     CDS             complement(2350..4140)
                     /locus_tag="PKK76_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67411.1"
                     /translation="MDPTLTRAAAHKAFGDLYRKARPPEVQEQVEKVIQSVSDVFARI
                     QRIEELDAAHARKQKGVETVERKRPSAGSGSGAAGAGAQRQAPKKPEPRRTGLLGAIL
                     GGGEIGVWGKDTETLTTGFLGRNLHISKKAEDIFSTLNEDVIVAVIKCFRVAARDGWT
                     QWDPPTYNVIVTAYQFFNEFITVAPMFKRVERQEVLAAESGKMQKMYAALIQYPNFRK
                     ILTDPFIKFVSESKELGGYTEALRNAMAFISSLDAKNPTLKNTILAFHGLAKKRVPTW
                     QEIVDEMGVGKPVLKRYRAPESVMQQIDGMIKKHHTQIAMRKAEMREIENLKRNFFDY
                     DGAGKVKTDFLNPIVAEVLRRIYNEKMVNDMTAKSHKTEPPKLLFVVMKDFDISSLYL
                     LESSVHARDGGNPIEIILFKQGLFKATIDEFNALLRDIEVFFKKNKNMSFHFHNLTEV
                     LKKDSGDEEMRRFVTLCRRATKLFRIVGTSFRTIWDNHIAAKSDTNQATQEKLARSKT
                     IPIESLAVGKRYIPFYDLEIVSNNRWNGMTVEGVIEQIVRNMYNYMLIFRDDELLGHL
                     SSPARLQAEIQSLIDELKKLGVEYDDSAPA"
     gene            complement(4142..4744)
                     /locus_tag="PKK76_02755"
     CDS             complement(4142..4744)
                     /locus_tag="PKK76_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67412.1"
                     /translation="MAKSAYRLLFLTAAVAWQLAAVPLFAEPNPDVQAVVSRMRSLGG
                     FKASISISSGGGGALRGNLSYSNGKVHLALSDGRVIAATGRELVVYNPSSSVAGKQLM
                     VPGGGGLGWLLTGFKTKVSGNTALLESENPSSNIQEVRLKWGAEHTLEQLSIRHKSSG
                     DWTTITLSNLRKVDAFPVSLFSYDAPAGSRTVENPLNQSN"
     gene            complement(4737..5675)
                     /locus_tag="PKK76_02760"
     CDS             complement(4737..5675)
                     /locus_tag="PKK76_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNL67413.1"
                     /translation="MAVLAVAELKGDSLKKISKELVSAGRTLGTPVVALLVNGNDAQA
                     KELLGAGADTVVKATLANYSPEGMANIVSAVAKEKGAKIVLAAHSVLGRDYAGRVAVQ
                     LGANVAADVVSIKGSLENVELRKPVYSGKAFANIRIKSPAVITVRPNSQEVKAHSGAE
                     AIETSSAGEGNVTGKITSTDMSGGSKIQLTDASIIVSGGRGIKGPENWPLLQALCDVT
                     GAALGASRAAVDAGWISHSAQVGQTGKTVSPNCYIACGISGAIQHLAGMGSSKYIVAI
                     NKDKDAPIFKVATYGIVDDLFKVVPAMTDEFKKVLG"
     gene            complement(5694..6455)
                     /locus_tag="PKK76_02765"
     CDS             complement(5694..6455)
                     /locus_tag="PKK76_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002748872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNL67414.1"
                     /translation="MKILVLIKQVPDTETSIKVSGAGIVDAGIKWIVSPFDEHALEEA
                     LRLKEKTGGTVIAASLGPDRVVEALRTAYALGADQAIHIKDDSYNVLDIGYAGSVLAK
                     FVEAEKPDLILTGHVAIDSQSSMVPSMIAQTLGCANINNAIELKVEGNKVVVKREIEG
                     GSAVMEASTPVVITAAKNLNEPRYPSLKGIMAAKKKQVDVKAADSFGSGFAKVEIVSL
                     ELPPPRPPGRIIDGDAPETKAKELVKALREEAKVI"
     gene            complement(6524..6886)
                     /locus_tag="PKK76_02770"
     CDS             complement(6524..6886)
                     /locus_tag="PKK76_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67415.1"
                     /translation="MQLSIPLLVFTVLCVGACAYLSVVIRQAYVHSLTSVEEKSAGII
                     HFYRARLRVLLSGAPDRMSILCGLHYLTVHLIPLVAGLSFYLKTDANFLVVILSIVWN
                     MQLFQRVFPGDSDAEDQT"
     gene            6943..8133
                     /locus_tag="PKK76_02775"
     CDS             6943..8133
                     /locus_tag="PKK76_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNL67416.1"
                     /translation="MEISLKPESDLFDPTPAHRELREVVRKFSEGPLREQARHGDESE
                     KFNIDLFRRLGSELGILGLTVPEDQGGAALGPVAEVILHEELSKSDPAFTLSVLAHEI
                     LFVNNLSVNGNDAQRKKYLPGVISAQKIGGMCMTEPGAGTDVLSLTTVAVKKGDRYVL
                     NGSKAWITNGYEGDYFLVYAKMDNSSNKSITKFIVESAYPGFRVGKKEEKMGMRASST
                     AVLSFEDMEVPAENLVGEENRGLVSMMRNLEIERIALAAQSLGIAERCIEVMAKYAVS
                     DRSSFGKHLIEHGQIQRLVSESYADWSAARCLVYHTAGRLSPNVRNSTAAAAAKLVAT
                     QMAERVGRNAIQVLGGNGYSREYPVERLYRDAVLLSIGGGTNEAMQKNIANDLIKIFA
                     GPSH"
     gene            complement(8185..9297)
                     /locus_tag="PKK76_02780"
     CDS             complement(8185..9297)
                     /locus_tag="PKK76_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67417.1"
                     /translation="MNKEIQKAFRDVVATSLNSDQVNALGQQMDRRFDIHELSGFGTK
                     IAIPGTAAAETLLHFLRTEERVVEFFERMLDQEGKFIYDSTVKLNYRDDFIRLLGKNK
                     WIFDVETKRFLRDPFFAEQLNFLKQLEMIDLRRSINLPAFSQQLQEDAVRLKLEDLNW
                     QVTIRTYRLTGEIATFLRDIISLLLMKQNLGSLSAEIYTCLNEMAINASKATYKVLFE
                     RHVAKPAGVDPKIEYNKFASMFREELEEHGDENLHKLAAAEDKYFDVIFRSTDANIAC
                     WTTNYTSISRPEKLRLLQRLNYTMFGENLTELMEDPHREGAGLGINLILTILGRLTPE
                     KHPLKPVFYQDRTKIGFVLKREALVNYVASRQTADT"
     gene            9468..10229
                     /locus_tag="PKK76_02785"
     CDS             9468..10229
                     /locus_tag="PKK76_02785"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL67418.1"
                     /translation="MNPLDDETLASRVFFPRKSAGRAPAGARDLMIPVARAELGARFY
                     EKDPAFRTVLYFHGNGETVPDYDRIAGLYHRSRLNLFVVDYRGYGFSTGSRPTLRSLS
                     EDPPIVSEFFLKQIRPAAPLPIIMGRSLGSSPATDIAVSNPQRYAGLILESGFADVRP
                     LLGLLGVQLSDAQEEEAHKLFSNAGKLSGIKIPVLILHGEEDHLIPAKHARANFEAAA
                     VKLKTLKIMQGAGHNDLLVQSDEYFSSIDQFVQKL"
     gene            complement(10245..10670)
                     /locus_tag="PKK76_02790"
     CDS             complement(10245..10670)
                     /locus_tag="PKK76_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67419.1"
                     /translation="MKNHGIGLLVYGAMSGTLMLFSTAMWNWLTMELTLQETLQIAME
                     SGVSMAPWIVVNYGAGVALVRLCASADTGFGSAMAAGFGLWLVLAPVNSEMASQLADT
                     ASLLFYTAPGSLLGTLAGAMVALAAPVKPLRQEMEMQIA"
BASE COUNT         2515 a         2696 c         2811 g         2943 t
ORIGIN      
        1 cctccgatgt tccaaccagt gtgcgtacaa cgtccgggtt tactccgctc atccagtccg
       61 tggatctgat cttcctttct ctctcatagc ccacatcata tgcgtcacta agcgtttctg
      121 aaatgggcac ggagggagga gcttgaacaa tctggccgtg gcacccgcag agaagcagaa
      181 gggccagaag caacctcatg caggatgaat tgcctcatcg catgacgtgt tcaaggaata
      241 atttcctcag tcacgcggaa agtggttgca tctgttgacc ggcgacctta gaaaaaggca
      301 tgcaagaatc gtctgaagtc tcgatgaaga ttcctttttt cgagagaaga agacgaattc
      361 gccgcccatg gactctgttg ttctttggca ttgctttcct gttgctaccg tttctcaatt
      421 atttgggaat cacaagccag ctacaaattt cgccacagat gccagtcgtc gcccttaagc
      481 ttctcaatcc tgtggaactg attctgcttt tcactcccat tctcgtcggg atcggacttc
      541 tcgcggtgaa gagatggggc tggtggttgt ttctcgcgta tgcggtcgtg ttgattgttc
      601 ataacatcta cgctctggct cgttctccgg gatactacaa cgcaggagct cttatccaga
      661 cgattcttgg agtggccgca gttttgtact tcgtgcgccg cgatatttct gcaccgtata
      721 tgcgaatgta tccgcgcgga tggagaattc aaaaacgcaa accggtagaa gtggaagtgc
      781 tggtggatgg cattcggcga tctacacgcg acgtaagtga ttcaggattg tatgtagcat
      841 gggaggattg ctatcgcgag ccaggagaag aagtccatct ggctttcttt ctggatggtg
      901 ctgagtataa atgcagagcc ggtgtggtgc gagtcgacga agatggcgca ggcattgcct
      961 atcgcggagt cactcgtgca tttaagaaaa acatcatgcg cgacattgct aagctggaga
     1021 acctcaacca accctagcgc gcgattctct tttggtcgtg caaacgccgc cttcgcaggc
     1081 ggcgttgtct cctggatcag tagactcttt ctggtcttcc cgagaatttt tcaaattttg
     1141 gttcaacatt cgccggtgcc cgaccaaagc aaatgcccat tctctcgtag agtgcaatgc
     1201 tctcaatgtc tacatgtctc tgaacgatcg cctctgcgac aggcatgtaa cgaatctttc
     1261 cgtcttgata ggaaatcaca gttacaccat tgattccttt gagatggagt tccactgcag
     1321 ccgcacctac ttcgagtccg aagtttgcgt ccacaggact tgtgaatccg cagcgcacta
     1381 gatggccggg gcgaacttcg cgaatttccg gaatctcata cactccttct acaaattggc
     1441 cagttcgttt catgaattct ttcacagccg gatctttttt tacacgatct tctagttgtt
     1501 tcaccacgta ttttccagca cccatgagct tcttatgacc gaacgcatcc gctgttgagc
     1561 tttcatctac gagttctttg ccggaagaat ctttgagtcc ttctgcaaca atcaccgcag
     1621 ccgtgccctt gcgcgcatcc gatgactcca ctcgtctgat gaaattgccc atcacatgct
     1681 tataaagaag gtcaaaatcg actggaactt ccggtatcaa aattgcgtcc gcaccggctg
     1741 cgattccacc gcgaaatgca gtgtgtcccg cataacggcc aaaaacttcc atgactacaa
     1801 ttcgattgtg agtgcggcca gttgtgtata gatcgcgcgt gaattccgcg atgcgtgcta
     1861 ccgtgctgtc tccacctaca ctgtaaggca tcaagtccag gtccatggtt ttgggagcgt
     1921 gtacgcatgg aattccctgc gaagcaagat ccacaactac gcttccggaa tcgtctccgc
     1981 ccgagatgac aagtgcatct attccgaatt tcttcaggcc ttcgcggatt ctgtcgtatt
     2041 ttttttcatc tttgatcttg gagatcttca cgcgtgagtt gcctgcttcc gatccagcca
     2101 ggtatacgtg aatgtcttcg atgcgagatt ctgttagttc gaccagaccc ggaaaatcta
     2161 cgagattgta aaggccagca tatccattcg ggatcaaata tgctttgatg ccgcgagata
     2221 gtgcgaggcg cgccgcgcca atgatgactc cgttgagacc gccgcagtct ccgccggaag
     2281 taaggatgcc gattttctga attgccatgc cctgaatcat cttcaggcta aaaatgagtc
     2341 aacgcaaatt caagcgggcg cgctgtcgtc atactcaaca ccgagtttct tgagctcatc
     2401 tatgagagat tggatctcag cctgcagtcg agcaggagac gaaaggtgtc cgagtagttc
     2461 atcatcgcga aagatcagca tgtagttgta catgttcctg acgatttgct caatcacacc
     2521 ttccactgtc atgccgttcc agcggttgtt ggatacgatt tccaggtcgt agaatggtat
     2581 atagcgcttt ccgactgcca gagactcaat gggaattgtt ttggatcttg caagtttctc
     2641 ctgggttgcc tgattcgtat cagatttggc agcgatgtga ttgtcccaga tcgtgcgaaa
     2701 cgaagttcca actatgcgaa agagtttggt cgcacgccgg cagagagtaa caaagcgtcg
     2761 catttcctca tccccgctgt ctttcttgag cacttcagtg agattgtgaa aatgaaagct
     2821 catgttcttg ttcttcttga aaaacacttc gatgtctctc agcaatgcat tgaattcatc
     2881 gatggttgct ttgaacaagc cctgtttgaa cagaatgatt tcaatgggat ttccgccgtc
     2941 gcgcgcgtgc acagagcttt ctagaaggta gaggctcgag atatcaaagt ctttcatcac
     3001 cacaaacaat agctttggcg gctcggtttt gtgcgatttc gcggtcatat cgttgaccat
     3061 tttttcgttg taaatgcgcc tgaggacttc cgctacgatc ggattgagaa aatcagtctt
     3121 aacttttcca gcgccgtcat agtcaaagaa attacgtttg agattttcga tttcacgcat
     3181 ttctgccttg cgcatggcga tttgcgtatg atgttttttg atcattccgt ctatctgctg
     3241 catgacggat tcaggagcgc ggtatctttt cagaacgggt ttgcccacac ccatttcgtc
     3301 tacgatttcc tgccaggtgg gaacgcgttt ctttgccaac ccgtggaacg ctagaatagt
     3361 gttcttaagc gtagggtttt tcgcatcaag cgatgaaatg aatgccatcg cattgcgaag
     3421 ggcttcggta tagccgccca gttctttgct ttctgaaaca aatttaatga atggatccgt
     3481 gaggatcttt ctgaagtttg gatattgaat cagagcagca tacatcttct gcattttgcc
     3541 cgattctgcc gcgagaactt cctgtctttc cactcgcttg aacatgggtg cgactgtaat
     3601 gaattcgttg aagaattgat aagcagtcac aatcacattg taagtgggag gatcccattg
     3661 ggtccaacca tcgcgagctg cgacgcgaaa gcatttgatt acggctacaa ttacatcttc
     3721 gttgagagtg gagaagatgt cctccgcctt tttggatata tgaagatttc ttcccaggaa
     3781 tccggtggtc agggtttccg tatcttttcc ccaaacacca atttctcctc cgccaaggat
     3841 agcacctaac aaccccgttc gtcgcggctc cggcttcttt ggtgcctgtc tctgtgcacc
     3901 ggcacctgcg gctccgctac cggagcccgc cgaaggtcgc tttctttcta ctgtttctac
     3961 gcctttttgt tttcgcgcat gtgccgcgtc cagttcctca attctctgga tgcgggcgaa
     4021 tacatcgctg acggattgaa tgactttttc aacttgctct tgcacttccg gaggacgtgc
     4081 tttgcggtat agatcgccga aagccttgtg cgcggcagcc cgcgtgaggg tcgggtccat
     4141 atcagttgct ctggttgaga gggttttcta ccgtgcgaga tcctgcgggg gcatcgtaag
     4201 aaaataaact tacgggaaag gcgtctactt tccgcaggtt gctgagagtg attgtggtcc
     4261 agtcgcctga gcttttatgc cgaatgctca gttgttcgag tgtgtgttcc gcaccccatt
     4321 tgagacgaac ttcctgaatg ttggaggaag gattttccga ttcgagtaac gctgtgttcc
     4381 cactgacttt tgttttgaat ccggtcaaaa gccagccaag tccaccgccg cccgggacca
     4441 tgagctgctt tcctgcgacg cttgatgatg gattgtacac aactagttcg cgtcccgtgg
     4501 ctgcaatcac tcgtccgtct gagagtgcga gatgcacctt accattgcta tacgaaaggt
     4561 tgccgcgaag agctcccccg ccgccggagg atatgctgat agaagctttg aatccaccca
     4621 gggatcgcat gcgagacacg actgcctgaa cgtccgggtt tggttctgca aataacggaa
     4681 cggcggccag ttgccaggca accgccgccg tcaggaacag aagtctgtaa gcagacttag
     4741 ccaagaacct tcttgaattc atccgtcatt gccggcacaa ctttgaacag atcgtctacg
     4801 atcccgtacg ttgcgacttt gaaaatggga gcatctttgt ctttgttgat ggccacgatg
     4861 tatttggaag atcccatacc agccagatgc tggatggcgc ctgagattcc gcatgcaatg
     4921 tagcagtttg gagaaactgt cttaccagtt tgtccaactt gcgcgctgtg agaaatccat
     4981 cccgcgtcca ctgcggcgcg gcttgcccca agcgctgcac ctgttacatc gcacagagcc
     5041 tgcagaagtg gccagttttc cgggcctttg attccacgac cgcctgaaac gatgatcgac
     5101 gcgtctgtga gctgaatctt ggatccacct gacatgtctg tgctggtgat ttttccggtt
     5161 acattgcctt ctccggctga ggaagtttcg attgcttctg cgccagagtg agctttcact
     5221 tcttgagagt tggggcggac tgtgatgact gccgggctct tgatacgaat atttgcgaac
     5281 gcttttccag agtagactgg tttgcgcagt tcaacatttt caagacttcc tttgatgctc
     5341 accacatctg ctgcaacgtt cgctccgagt tgcactgcaa cgcgcccagc gtagtcacgt
     5401 cctagaacag aatgagctgc cagaacgatc tttgcgcctt tttcttttgc aaccgcggaa
     5461 acgatgtttg ccattccttc cgggctgtaa tttgccagag tggctttaac aactgtatct
     5521 gcgcctgcgc ccaggagttc ttttgcctgt gcgtcattgc cgtttacaag caatgcaacg
     5581 actggagttc cgagggtgcg cccagctgaa acaagctctt ttgagatttt cttcagcgaa
     5641 tcgcctttga gttctgctac tgcgagtact gccattgtat tctacctctt tcctcagatt
     5701 acttttgctt cttcgcgaag cgccttaacc agttctttcg ccttcgtttc aggtgcgtca
     5761 ccgtcgatga tgcggcctgg tggacgagga ggtggaagtt cgagagaaac gatttctact
     5821 tttgcgaacc cgcttccgaa tgaatcagct gctttcacat ctacctgctt cttcttggct
     5881 gccatgattc ctttcagaga aggatagcga ggttcgttca ggttttttgc tgctgtgatc
     5941 actactggag tggaagcttc catgactgcg cttccgcctt cgatttcgcg ttttacaact
     6001 actttgtttc cttcaacttt tagctcgatc gcgttgttga tgttcgcgca tccgagagtc
     6061 tgtgcgatca tggatggaac catggaagac tgagagtcaa ttgcaacgtg gccagtcagg
     6121 atcagatctg gtttttctgc ttctacgaac ttcgcgagca ctgaaccagc gtagccaatg
     6181 tccagaacgt tgtaggaatc gtctttgatg tgaattgcct ggtctgcgcc gagcgcatat
     6241 gcagtgcgaa gcgcttccac tacgcggtcc gggccgagtg atgccgcaat tacagtcccg
     6301 ccggtttttt ctttcaggcg aagggcttct tcgagcgcgt gttcatcaaa aggcgataca
     6361 atccatttga tgccggcgtc tacaattcct gcgccagaaa ccttgatgct ggtttccgtg
     6421 tcaggaacct gtttaataag tacgagtatc ttcattgagg atctccaatg gataaaactt
     6481 ggattctacg actttttggt ggtccgggcc ggggtcaatg actttacgtt tggtcttccg
     6541 cgtcagaatc gcctgggaaa acgcgctgaa atagctgcat attccataca atagaaagaa
     6601 tcacgacgag gaaattcgca tctgtcttta gatagaaaga aagccccgcg acgagaggaa
     6661 tcagatgaac cgtgaggtaa tgaaggccgc aaaggatgga catgcgatcc ggggctccag
     6721 aaagcaaaac gcgcagtctg gcgcgataga aatgaatgat cccggcgctc ttctcttcca
     6781 cactcgtgag tgagtggaca taagcctgac ggatgaccac gctcaggtag gcgcaagcac
     6841 ccacacaaag cactgtaaat actagaagtg gaatggacag ttgcatgaat cgagaagaga
     6901 aaaaattgtt tatgggacag tgcaaggaaa acttttcatc ctatggaaat atcgctgaag
     6961 ccagagtccg atctttttga tccaacgccc gcccatagag agcttcgtga ggtggttcgc
     7021 aagttcagcg aaggcccatt gcgcgaacag gcgcgtcatg gagacgaatc agaaaaattc
     7081 aacatagact tgtttcgcag gctgggcagc gagcttggaa ttctcggcct cacagttcct
     7141 gaagatcaag gaggagcggc tctgggccct gtcgcagaag tgattctgca cgaagagctt
     7201 tccaaaagcg atccggcgtt tactttgagt gttcttgccc acgaaattct atttgtgaat
     7261 aatctttccg taaatggaaa cgatgcgcag agaaagaagt atttaccagg ggtcatttct
     7321 gcgcagaaga taggtggtat gtgcatgacg gaacctggtg ctggaacaga cgttctgagt
     7381 ttgaccacgg ttgccgtgaa aaaaggcgat cgttatgtgc tcaatggatc caaagcctgg
     7441 atcacaaacg gatacgaagg cgactacttc ctggtctatg cgaagatgga caattcttcc
     7501 aataagagta ttaccaagtt catcgttgag tctgcatatc caggatttcg cgtcgggaaa
     7561 aaagaagaga agatgggcat gcgtgcgtcg agcacagctg ttctttcttt tgaagatatg
     7621 gaagtccccg cggaaaacct tgtaggagag gaaaaccgcg gcctggtttc catgatgcgc
     7681 aatctggaga tcgaaagaat cgctctggcc gcacaatcac ttggaatcgc ggagcgctgc
     7741 attgaagtga tggcaaagta cgcagtgagc gatcgatcca gttttggaaa gcatctcatt
     7801 gaacacggac aaatccagcg actggtttct gaaagttatg cggactggtc tgccgctcgc
     7861 tgtcttgtgt atcatacagc tggaagatta tcgcctaacg ttcgaaattc gactgcggca
     7921 gccgccgcca agcttgtggc aacacagatg gcggaaagag ttggaagaaa tgcgatccag
     7981 gtactgggcg ggaatggtta cagtcgtgaa tatcccgtgg aacgtcttta ccgcgacgcg
     8041 gttctattga gcattggtgg cggaaccaac gaagccatgc agaagaacat tgcaaatgat
     8101 cttattaaga tcttcgctgg tccttcgcac tgaagggaaa gtgattctct aaacggagta
     8161 attagacgca agatgaagcg gatctcaagt atccgcggtc tggcgagatg cgacgtagtt
     8221 tacaagtgct tcgcgcttga gtacaaagcc aatcttagtt ctgtcctggt agaatacagg
     8281 tttgagcgga tgtttttccg gagtgagtct tccaaggatc gtcaaaatca aattgatgcc
     8341 cagtcccgcg ccttcgcggt gaggatcttc catgagctcc gtcaggtttt ccccaaacat
     8401 ggtgtagttg agtctttgaa gaaggcgtaa tttttccggt cttgagatgc ttgtgtagtt
     8461 ggtagtccag caggcaatgt tcgcgtctgt ggatctgaaa attacgtcga agtatttgtc
     8521 ttctgctgcc gcgagcttgt gaaggttttc atccccgtgt tcttccagtt cttcgcggaa
     8581 catggatgca aatttgttgt attcgatttt tgggtccaca cctgccggtt tcgctacgtg
     8641 tctttcaaag agcactttgt aagttgcctt ggatgcgttg atagccattt cattgagaca
     8701 ggtgtaaatc tctgcggaaa gagaccccag gttttgcttc atcagtaaaa gcgaaatgat
     8761 atcgcgcaag aacgttgcga tttctcccgt caggcgatat gtacggatgg ttacctgcca
     8821 gttcagatct tccagcttga ggcgcaccgc atcttcctgc agttgctgag agaaggcagg
     8881 tagattgatg gagcggcgaa gatctatcat ctcaagttgt ttgaggaagt tgagctgctc
     8941 tgcgaagaat ggatctctga gaaatctttt tgtttcaaca tcaaagatcc atttgttctt
     9001 cccgagcagg cgaatgaaat catcgcgata gttcaatttg accgttgagt catatatgaa
     9061 cttgccttcc tggtcgagca ttctctcgaa gaattcaacg actctctctt ctgttctcag
     9121 gaaatgcaaa agtgtttccg cagcggcggt cccgggtatg gcgatcttgg tgccaaatcc
     9181 agacagctca tggatatcaa atctccggtc catctgctgg cccagcgcgt tgacctggtc
     9241 tgagttcaga gacgttgcaa caacatcgcg gaatgctttc tggatttcct tgttcatcag
     9301 gtggtggcat ttgtgaaata tcgccgcatt ccagtcaaga actagtgccc ggattttttg
     9361 gattgaggcc tgcccgggcc ccctgtgaat gagtcatgtt cctttgtgct cggattggcc
     9421 ttttggtagt tctactggtg catggttgca cggaaaggag agcggttatg aatccgttag
     9481 acgatgagac tcttgcctct cgggttttct ttccgcgtaa gagtgcaggc cgggctccgg
     9541 cgggggccag ggatttgatg attccagttg ccagggcaga gctcggcgct cggttctatg
     9601 agaaggatcc agcgtttcgt actgtgctct atttccacgg gaacggagag actgttccag
     9661 actatgatcg cattgcaggg ctctatcata gatccagact gaacttattc gtagtcgact
     9721 atcggggcta cggattcagc accggaagtc gtcctactct aagatcgctt tcagaagatc
     9781 cgcctatagt cagtgagttc ttcttgaagc agattcggcc tgctgcgccg cttcccatca
     9841 taatggggag atctctgggc agtagtccag ccacggatat tgccgtgtcc aatccgcaac
     9901 gttatgcggg cttgattctt gagagcgggt ttgcggatgt caggccgctt ctcgggctcc
     9961 tgggggttca gctgagtgat gcacaggaag aggaagccca caaattgttt tccaacgcgg
    10021 gcaagctttc cgggatcaaa attccggtcc tgattctaca tggagaagaa gatcacttga
    10081 ttccggccaa acatgcgcga gccaactttg aagcggctgc agttaagctg aaaactttaa
    10141 agatcatgca aggcgcagga cacaatgacc tgcttgtgca gagcgatgaa tacttctcat
    10201 cgatcgatca atttgtgcaa aagctttaaa gctcttctcc aacgttaggc gatctgcatc
    10261 tccatttctt gtcgcaatgg tttgacgggt gccgccagtg ctaccatcgc ccctgcgaga
    10321 gttccaagca ggcttccagg ggccgtatag aatagaagag acgccgtatc cgcaagctgg
    10381 gaagccattt ccgaattcac aggagccaga accagccaga gaccaaaacc cgcggccatg
    10441 gcagatccaa agccagtgtc cgcagaggcg cacagcctta cgagtgctac cccggctccg
    10501 tagtttacga cgatccaggg agccatggac actccgctct ccatggcgat ttgcagcgtt
    10561 tcctggaggg tcagctccat tgtcagccaa ttccacattg ctgtggaaaa gagcattaga
    10621 gtgccgctca ttgctccata aaccagcaat ccgatcccat gattcttcat aaatcgcccc
    10681 ccatcctgtt tgaggagcaa gatgtgtacc ccaaaaatat cgcggctcgc gctatttttt
    10741 gggacccggt tcaggctgca tcgggtctta ccagtcgtgc gaggatgatt cccgcatgca
    10801 aaacccgcag gtgtactttg tgcccttgcg aaggagaaaa atatgatgag acaatttcta
    10861 tttgccggcc gcagacattg cggcgggcat cacaaattca gagaagaggc agggttcatg
    10921 ggtggtccat ttggtgtccg ccggccgttg cgtcatctag cttac
//
LOCUS       DAORFF010000038        10932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2718_length_10932_cov_3.662223, whole genome shotgun sequence.
ACCESSION   DAORFF010000038 DAORFF010000000
VERSION     DAORFF010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10932
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2718_length_10932_cov_3.662223"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PKK76_02795"
     CDS             <1..524
                     /locus_tag="PKK76_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNL67420.1"
                     /translation="IVLPGLYFLLSAADVPFADNVGMGDLLGLGCAATYGLYMVLLDR
                     ATRHEDVVLIVFFQIVFAAACFVLAFSISDEKMHLQVSTRYITAIAYLSIATTVLTTY
                     LQSRFQKLVSAARAGVLYSMEPVFASIFAFALLGENPGVRGYAGGGLILFGVILSETW
                     PFFMHRLRRYSPG"
     gene            731..1213
                     /locus_tag="PKK76_02800"
     CDS             731..1213
                     /locus_tag="PKK76_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67421.1"
                     /translation="MLQWHQVAGLATWGLWLATNLKGEEAIHSLRRSGEPVANAVLLA
                     DPRNNWPLYYAILNNSEWEAKRGGSTHRTLAGLTAGAYALTAGLALLAPSRPAESDSY
                     DSITAHKWLALLHFAALASLPALGKRIEEGGPKAAKRMQKVGWGGFGALTVAVGVFYF
                     "
     gene            1283..1837
                     /locus_tag="PKK76_02805"
     CDS             1283..1837
                     /locus_tag="PKK76_02805"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNL67422.1"
                     /translation="MKFWLEQSFRIFIIWALVGSTAAAQAQEASSVSFFADHSFKVIE
                     GRCADVAVSGSATKSGSTFTAQNLTIQCSIPKMTTGNRNRDSNMWEMLGYPQVQQITV
                     LVPSASAKAGKAPVSFIVRMHGKEKSMTSDMNFEEQSGSVVVSGDFTVSLAEYDVTPP
                     KLLMLKVGDAVLVRFRIAVPVQGK"
     gene            complement(1806..2687)
                     /locus_tag="PKK76_02810"
     CDS             complement(1806..2687)
                     /locus_tag="PKK76_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67423.1"
                     /translation="MPSSITKQRIDDLDAMTADALFLDDLPADTIALARLLECAAAGS
                     MAVLEFNESIRRASEWKEVFQALGFKVLKIGRRSGRLIATLYKPVHPASLRRWSVILR
                     HSGSANTFARLYEWIAFFQRFDSIGEAEIILVEDDPLEGNVKMLNSIASEGFFLRKRH
                     YRPVGLMHSFATGIHFSSGRKIILDADPRIQPSESLSLLEASYPLDYDRLAYFVIGNP
                     TDGQTDLREAHARRRRRFFSSLFAKSENRFTFSLLTGEGARSGMPSRSSLARSVRGLK
                     TVQVPVTITSPALAQQS"
     gene            complement(2777..3160)
                     /locus_tag="PKK76_02815"
     CDS             complement(2777..3160)
                     /locus_tag="PKK76_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNL67424.1"
                     /translation="MKPYLIALSLASALLVAYCSEEKKPAEQTPVQMPPEAAQGKEIY
                     IAQNCNGCHGETGLGDGPAGQTLNPKPRNYRDIYAYRKGSSLDQIAAMLEFGLEGSPM
                     KGYPHLTLEKRKLLAAYVVWLQKQP"
     gene            3352..>3663
                     /locus_tag="PKK76_02820"
     CDS             3352..>3663
                     /locus_tag="PKK76_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNL67425.1"
                     /translation="MVGGSSEFVAPEFRALFEASPGAYLVLNPDLIIVAATDSYIRAT
                     MTRREDLLGLHVFEAFPDNPDEKNATGVANLRASLQRVLESKKSDAMAVQKYDIKTPG
                     GH"
     assembly_gap    3664..3763
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3764..4854
                     /locus_tag="PKK76_02825"
     CDS             <3764..4854
                     /locus_tag="PKK76_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL67426.1"
                     /translation="SRMQWPFRNTISKLLAGMLNCDLLARDDVRDKQKGIDQIRKTAE
                     RAANMTHQLLAFSRTQVFQPRVVSMNTIIRDLEPILKRLTREGMDLRLSLDENLCNVV
                     ADVTQVEQILINLVANARDAIPGAGRIEVQTETVTLDEMSARGDPAACPGKYARLCVR
                     DTGIGMDAVTQARIFEPFFTTKQAGKGTGLGLATIYGIVAQNQGTIRVISEIRKGTSF
                     NVFWPAVEAPVQMILRSEKAQPVLRSARILVVEDEDDLRNLICDTLMAHDFQVENAEN
                     GEVALSKIEKSSTPFDLIITDVRMPVMTGSALKRELDRRGIRVKVLFMSGYSQEALFD
                     LANREEHAFLEKPFTGTDLLSKIDAILR"
     gene            complement(4866..5846)
                     /locus_tag="PKK76_02830"
     CDS             complement(4866..5846)
                     /locus_tag="PKK76_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNL67427.1"
                     /translation="MRTLVTGATGLIGYNMVQQLVAGGHEVRVLARSVEKARALFSNT
                     CTILQGDVTEPQSLAPAMEGCEIVFHAAGFPEQWMKDPAIFQQVNVEGTRNVLNAGTS
                     AGVRRVVYTSTIDVFAARAGESYDESVLDTQPKGTYYERSKQDADRVAVEFLSRGMDI
                     VFIHPAGLYGPGPMGSPGVNQLIIDLKKGKIPMLLPGGFPLVYSEDVARGHLLAASSA
                     AAGDRFILSEGYYDLTEVATIVCKNLGIAKTPPVMPLWMGKAIAGAGEAISSITGKPP
                     LIPKGQLHFMQWKAIPKSERANVVLGWKALPFEEGVKKTISFLESKGLLS"
     gene            6073..7689
                     /locus_tag="PKK76_02835"
     CDS             6073..7689
                     /locus_tag="PKK76_02835"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNL67428.1"
                     /translation="MKKLNLFIIIGAALVVGMCNSGKQAIREELSIDQAPRIAHEGER
                     GRITNPSSKLSNADGKDDQSWRYNPEFNTEAYSRIEDNAFLDVKQNALSTFSVDVDTA
                     SYSNVRRFLTDGQLPPKDAVRIEEMINYFSYNYAAPTGPHPFSVTMEHSAAPWNPDHE
                     LVLIGLQAKKIAEDKMPPRNLVFLVDVSGSMDEPNKLPLLKRSLALLADQLTEKDHVA
                     IAVYAGAAGLALEPTSGADKGKIKAALAELHAGGSTNGAQGIELAYATAKKFFRKDGI
                     NRVILATDGDFNVGVTNEGDLTRLIEEKRKDGIFLTVLGFGMGNLKDSTMEKLADKGN
                     GNYAYIDNFSEARKVLVEQAGGTLVTVAKDVKIQVEFNPAQVSAYRLIGYENRLLRNE
                     DFNNDQKDAGDIGAGHSVTALYEIVRVGKPIQTPGVDPLRYQKPGTAVTNLNGELLQV
                     KLRYKQPDSDTSTLLSQTLSSESPGSTENLRWASGVAMFGMVLRESPYKGNATIESAY
                     SLASKSTGNDPGGYRSEMLRLLRAAEALKR"
     gene            complement(7742..9880)
                     /locus_tag="PKK76_02840"
     CDS             complement(7742..9880)
                     /locus_tag="PKK76_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNL67429.1"
                     /translation="MRNIGRLIPLLILALTIGCDKKILIGVSLPLSGPNAETGKEMLQ
                     AVQLHVDSINEKGGIGGKKVGLVIKDDEDDPAKVKKAAEELAGNSNVVGVIGHLNSPT
                     VIAAAEVYDKTDLVAITPVSTNTDLSTKTKNIFMLNRDGVYQGQFMVDYLKGAMGKSH
                     PIVFVQESRYGLRHWKGFEERASELSITPVKVIRFKNEDLGTEAFAANLTQDPTADSI
                     ILLTDDNADVGLKLLTIIDNSKVAPEDTVMLAPESYLNPKYTTKEAERYTDTLYVCTP
                     FLWEIANQTASAMREAYEAKYKVQPGLGGAMAFDAAMLLTTAIAEKGTDRKLVHEYLL
                     EQKRKDLQAKRKDLLVGATAELVFGEGRFADRDLFVAKVEHGRYKVAFTQLATVKEPY
                     VIAQLPERIANGSVIDLAGTPYHVVDVIFMGLDILKLNSVNTKDMRFEVEFFMWYKWA
                     SKAVDPSQIDAVNGAGDLKTTLLKERTKKGVTYRALRCKGTFNTQMDFSQFPFDRQGL
                     EMSFVNKVKHSAHLFIVPDTRHMTHDPLKGLPLDWTFIQKKNYTGLLRYDSTFGDPDY
                     RMGAGYKSRILFSTIGFRLDVQRVPTGFAFTMFVPLVVILVASFFVISAPIEELKGRL
                     PLGLTALLTVVLSRMALASQTPSVAYIMRSDYFYLTAFLYVMGLIAVLSGVIWLMAKK
                     NDKGARLLHKFFVMVVSPLVAVVLLLQFIL"
     gene            9972..10775
                     /locus_tag="PKK76_02845"
     CDS             9972..10775
                     /locus_tag="PKK76_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNL67430.1"
                     /translation="MRLAFCFAFLISSSLLADESLHPGPAFVFGTQVNVREKGATTAA
                     VKFMLPIGAKVTIETATQEKVIQGARSDFWYQIKTADGRNGFVFGSLLTALGEELGDG
                     RLLLVGWAENAKDSFEFRIVQKGSLVSSRAFALLGYSQDPAAANYCHTMELHRNLFGQ
                     GNHAISLLSFAGSEMDAGFSNGAYAFILRGNTIDLVSQGQGGADAPAYADYTFSFRPG
                     IIEEKFEEVGDIQDGIEIGEGYTRQFKLVAGKIAASEKQIYKIRRPVPQ"
     gene            complement(10776..>10932)
                     /locus_tag="PKK76_02850"
     CDS             complement(10776..>10932)
                     /locus_tag="PKK76_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67431.1"
                     /translation="IGEVCLAIEMGCDSVDIGKTIHPHPTLGETVGLTAEVAHGSCTD
                     LMPVRKK"
BASE COUNT         2598 a         2618 c         2841 g         2775 t
ORIGIN      
        1 ccattgtttt gcctggtctg tactttctac tctctgcggc cgacgttcca tttgcagata
       61 acgttgggat gggcgatcta ctgggtctcg gttgcgcggc cacgtacggt ctgtatatgg
      121 tgctcctcga tcgcgccacc aggcacgaag acgttgtgct gattgttttt tttcagatcg
      181 tttttgccgc cgcctgcttc gttctggctt tctctatttc agacgaaaag atgcatctgc
      241 aagtttctac cagatacatc actgctatcg catatctttc cattgcaacc accgttctga
      301 ctacgtactt gcagagtcgg tttcagaaac ttgtctcggc ggcgcgtgca ggtgtgctct
      361 attccatgga accagtcttt gcctcgatct tcgcctttgc attgttaggt gaaaacccgg
      421 gggtgcgagg ctatgctggt ggcgggttga ttttatttgg cgtgatcttg agcgaaacct
      481 ggccattttt catgcaccgc cttcgcagat actcccccgg gtaagaattc gtaactggcg
      541 cctcaacggg tggacgattt cgctgaatct cgttctcaac tcgaattcat aaaaaatcat
      601 ttttcctttg cttttgattt ggacgtccgt tttccttgta cctatgatcc gcagatggtt
      661 tgtccttgtt ttcttatatc tattcataca gtcgtccatt cgcgcagacg aattgtccac
      721 gagacgggaa atgcttcagt ggcaccaggt agcaggcctg gcaacgtggg gactctggtt
      781 ggccacgaac ctgaagggag aggaagccat ccattcgctg cgcagaagcg gggagccagt
      841 ggcaaatgcg gttctgcttg cagatcctcg caataactgg ccactttact atgcaattct
      901 caacaattcc gagtgggagg ccaaaagagg ggggagcact cacaggacac tcgcggggct
      961 gacggccggg gcctacgcgc tgacagccgg gcttgcactt cttgcacctt ctcgtccggc
     1021 agaatctgat tcctatgaca gtatcacagc gcacaaatgg ctggcgctct tgcactttgc
     1081 agctcttgcc agtttaccgg cgcttgggaa acggatcgaa gaaggtgggc caaaggcggc
     1141 caaaagaatg cagaaagtgg gatggggtgg ctttggagcg ttgacagttg ctgtcggtgt
     1201 tttctatttc tagcaacgtc gcagagcagc catcaaggga agattgaaag tatagtgaaa
     1261 attgaaagta caatgaggca atatgaaatt ctggcttgag caatccttta gaatatttat
     1321 aatctgggca ctcgtcggtt caacggcggc cgcccaggcg caggaagcaa gctccgtctc
     1381 tttttttgcg gaccacagct tcaaagtcat tgaaggaaga tgtgcggatg ttgcggtgtc
     1441 cggttctgcc acaaagagcg gcagcacgtt tactgcgcaa aacctcacca tccaatgttc
     1501 tattcctaag atgaccacgg gcaatcgcaa ccgggattcc aatatgtggg agatgctggg
     1561 ctatccccag gttcagcaga tcacggttct tgttccgtct gcgtccgcga aggcgggaaa
     1621 ggctcctgtt agctttatag ttcgcatgca cggcaaagag aagagcatga cttctgacat
     1681 gaattttgaa gagcaatcag gcagtgtggt tgtatcggga gatttcactg tgtctcttgc
     1741 ggaatacgac gtaactccgc caaagctgct catgttgaag gtgggggacg cagtgctggt
     1801 tcgtttcagg attgctgtgc cagtgcaggg gaagtgatcg taacgggcac ctgaaccgtc
     1861 tttagtccgc gaacacttct ggcgagtgag ctgcgcgatg gcatccctga acgagcgcct
     1921 tctcccgtga gtagcgagaa agtgaatctg ttttcgcttt ttgcgaacag cgaagagaag
     1981 aaccggcgtc tgcgcctggc atgtgcttcg cgcagatctg tctggccgtc cgttggattt
     2041 ccaattacga aatacgcaag gcgatcgtag tcgagcggat aagatgcttc cagaagcgat
     2101 agcgattctg agggctgaat gcgcggatcc gcgtcgagga tgattttccg tccggatgaa
     2161 aaatggattc ccgttgcaaa agagtgcatc agcccaacag gcctgtagtg tctctttcgc
     2221 agaaagaagc cttcgctggc gatgctgttt aacatcttca cgttgccttc gagtggatca
     2281 tcttccacga gaatgatttc tgcttcccct atggaatcaa atcgttggaa gaatgcgatc
     2341 cattcgtata accgcgcgaa ggtgtttgct gagccagaat gacgtagaat gacactccat
     2401 cttcgcagac ttgccgggtg aactggtttg taaagcgtag caatgagccg accggagcgc
     2461 cttcctattt tcagaacttt gaaccccaga gcctggaaga cttctttcca ttcagaggcc
     2521 cgccggatgg actcattgaa ttcgagtact gccatggagc cagcggcggc gcattctagc
     2581 agccttgcca gtgcgatcgt atctgctgga agatcgtcca gaaacaaagc atccgcggtc
     2641 attgcgtcca gatcgtctat gcgctgtttg gtgatcgaag aaggcattgc ctccgccgcg
     2701 agccttgagc ccggcaggta tcccagcaca gaagtgtgtg ccgggagaat tttcagtagt
     2761 tctttgtgtg gagttttcag ggttgtttct gtagccagac tacgtaagct gccagcagtt
     2821 ttctcttctc cagagtcagg tgaggatagc ctttcatggg ggatccttcc agtccgaatt
     2881 cgagcatggc tgcgatttga tcgagagagc tgccttttct gtaagcataa atgtcacggt
     2941 agttgcgcgg tttaggattg agagtttgtc ctgccggccc gtctcctaga cctgtttccc
     3001 cgtgacaacc attgcagttt tgcgcaatgt agatttcctt tccctgggct gcttctggcg
     3061 gcatctgcac cggcgtctgc tccgctggtt ttttttcttc cgagcaatag gccacaagga
     3121 gggcggacgc aagcgacagg gcaatgagat aaggcttcac gggatgaacg ttgcggacat
     3181 ggtgcctctg tcaaggcgat tgcagatggg agcctgttta caatcgcata gttcatcccc
     3241 ctatcgcaca cttttgtgcg ctcggttaga gaaatccagt cggcgcctaa cgggaacgaa
     3301 aatgcagata aaaggatgga cctttttcgg ggagcatact ctatctgaag gatggttggg
     3361 ggatcatctg aatttgtggc tcctgaattc cgcgctttgt tcgaagcaag ccctggcgcg
     3421 tacctggttc tcaacccgga tctgatcatt gtcgcggcca cggactcata cattcgcgca
     3481 actatgaccc gccgtgagga tttactcggt ctgcatgtgt ttgaagcgtt tccggataat
     3541 ccggatgaaa agaatgccac gggtgtggca aatctgcgcg catctttgca gcgcgttctg
     3601 gagtcaaaaa agtcggatgc aatggccgtt cagaaatacg atatcaaaac tcctggcggg
     3661 catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaagtcg gatgcaatgg
     3781 ccgttcagaa atacgatatc aaaactcctg gcgggcatgt tgaactgcga tcttcttgcg
     3841 cgtgatgacg ttcgagataa gcagaaagga atagatcaaa tcagaaagac agcggagcga
     3901 gcagcgaata tgactcacca gctcctcgca tttagcagaa cacaggtctt tcagcccagg
     3961 gttgtgagca tgaacacgat catcagggac ctggagccaa tcttgaaacg actcacacgc
     4021 gaaggaatgg acctcaggct ttcgcttgat gaaaatctct gcaatgtagt cgcagacgta
     4081 acgcaggtgg agcagattct catcaacctt gtagcgaacg cgcgcgatgc aatcccgggt
     4141 gccggtagaa tcgaggtgca aacggagaca gtgaccctgg atgaaatgtc cgcgagggga
     4201 gatccggcag cctgtcccgg aaagtacgcg cgactgtgcg tgcgggatac ggggattgga
     4261 atggacgcgg tcacgcaggc gcgaattttt gaacctttct tcacaaccaa acaagccggt
     4321 aaaggcacgg ggctggggct ggccaccatc tatggtattg ttgcacagaa tcagggaacc
     4381 attcgcgtta tcagcgaaat tcgaaaagga acgtcgttta acgtcttctg gcccgcagta
     4441 gaggcgcccg tacaaatgat cttgcgaagc gagaaggcgc agccagttct tcgatctgca
     4501 agaattctag tggtggaaga tgaggatgac ttacgtaact tgatttgcga tactctcatg
     4561 gcgcatgact ttcaggtgga gaacgctgaa aacggggaag tcgcgctttc aaagatcgag
     4621 aaatcatcca caccatttga tttgatcatt acggacgttc gcatgcctgt gatgacagga
     4681 tctgccctca aacgggaact ggatcgcaga ggaatccgtg tgaaggtgct ttttatgtcc
     4741 ggctattctc aagaagcact gttcgatctt gcaaatcgcg aagagcacgc ttttcttgag
     4801 aagcctttca ccggaacgga tctactctcg aagatagacg caatattacg ttagccggaa
     4861 ttgcactacg atagaagccc ctttgattcc aggaatgaaa ttgttttttt gactccctct
     4921 tcgaaaggga gagccttcca tcccaggaca acatttgctc gttcgctttt tggaatggcc
     4981 ttccattgca taaaatgaag ctggcccttt ggtatgagtg ggggcttccc cgtaatggaa
     5041 gaaatcgctt ctccagctcc cgcaattgcc ttacccatcc acagaggcat caccggagga
     5101 gtcttcgcaa ttcccaggtt cttgcacaca atcgttgcca cttcagtcag gtcgtagtag
     5161 ccttcgctca gtatgaatcg atcaccggcc gccgcagaac tggccgcaag cagatgcccg
     5221 cgcgctacgt cttccgaata gaccagagga aaaccaccgg gtagaagcat ggggattttt
     5281 ccctttttca aatcaatgat gagctggttg actccgggag atcccattgg cccgggaccg
     5341 tatagcccgg ccggatgaat gaacactatg tccatcccgc gagagaggaa ttcaactgct
     5401 accctgtccg catcttgttt ggatctttcg tagtaggtgc ctttgggttg tgtatccaga
     5461 acggattcat catatgattc tccggcacgg gcggcgaata catcgatggt gctggtatat
     5521 acaacacggc gaacgccggc agacgtacca gcattcaaaa cgtttcgcgt accttctaca
     5581 ttgacttgct ggaaaatcgc aggatccttc atccattgtt cggggaaacc ggcggcatga
     5641 aatacgattt cgcagccttc catggcgggg gcaagactct gtggttccgt tacatctccc
     5701 tgcaggatgg tgcatgtatt cgaaaagagt gcacgtgctt tttccacgct gcgcgccagg
     5761 actcggactt catgtccgcc tgctacgagc tgttgcacca tgttatatcc aatgagaccc
     5821 gtggcgcctg taacgagagt tcgcattcct caacggatca ttttctgcag tgacaggtca
     5881 aagcaaaatc ccaggcatgt ctttgttttc gtattggaga cgatctcatc caccgggggg
     5941 ttcgcgtaat ttcagaaagt agagagaatt tttaagaaac tgcaacggat cgtctgctgc
     6001 tttttttgtt ccgaaaaact tgaataaatt ccaccttttt ttcgtcgacc ccgtctacct
     6061 ttcggaggca gtatgaaaaa gttaaatttg tttattatca ttggtgccgc gctcgttgtc
     6121 ggcatgtgca attctggaaa gcaagcgatt cgtgaagaac tctcgatcga tcaggccccc
     6181 cgcattgcac acgaaggaga acgggggcga atcacaaatc cgtcctcaaa gctctccaac
     6241 gcagatggga aggatgatca gagctggaga tacaaccctg agttcaacac ggaagcctac
     6301 agtagaatcg aagacaatgc gtttctggat gtaaagcaga acgcactttc cacgttctcc
     6361 gtggatgtgg atacagcatc gtacagcaac gtcaggcgat ttttgacaga tgggcaactt
     6421 ccgccaaagg atgcggtgag aattgaagag atgatcaact acttctcgta caactatgcc
     6481 gcacctactg gaccgcatcc attttctgtt accatggagc attcagcggc tccctggaat
     6541 ccggatcatg aactggtgtt gattggttta caggccaaaa agattgcaga agacaagatg
     6601 cctccgcgca atcttgtatt tctggtggac gtttccggtt ccatggacga accaaacaag
     6661 ctccctttgc tcaaaagatc tctcgcgtta ctcgcggatc aactcactga aaaggaccac
     6721 gtagcgatag cagtgtatgc tggagcggcc ggtcttgctc tggaaccaac gtcaggtgca
     6781 gacaaaggaa agattaaagc cgcgcttgct gaattgcacg caggtggcag cacaaacgga
     6841 gctcagggaa ttgagctcgc atacgcgact gccaaaaagt ttttccgcaa ggacggaatc
     6901 aaccgggtca ttctggccac ggatggcgat ttcaacgttg gtgtaaccaa cgaaggtgat
     6961 ctgacacgtt tgattgaaga aaagcgtaag gatggcatat tccttacagt tcttggtttt
     7021 ggaatgggaa atttgaaaga ttctaccatg gagaaactgg cggacaaagg aaacggaaac
     7081 tatgcttaca ttgacaattt ttccgaagca cgcaaggtac ttgtcgagca ggcgggtgga
     7141 actctggtca cggtcgcaaa agacgtgaag attcaagtgg agttcaatcc agcgcaggtg
     7201 agcgcctatc gtttgattgg atatgaaaat agattgttgc gtaacgaaga cttcaacaat
     7261 gatcagaaag atgcaggcga cattggtgcc gggcattccg tgacggccct ctacgaaata
     7321 gtgcgagtgg gtaagccaat tcaaactccg ggagtagatc cacttcgtta tcagaagccg
     7381 ggaacggctg tgaccaatct gaacggagaa cttcttcagg tgaagctgcg ttacaaacag
     7441 cctgattctg atacaagcac tttgctgagt cagacccttt cctctgaatc gccgggatcc
     7501 actgagaatc ttcgctgggc ttcaggtgtt gctatgtttg gtatggtgct tcgcgaatca
     7561 ccttacaagg gaaatgcgac gatcgagagc gcatactctc ttgccagcaa gtcgacagga
     7621 aacgatccag gtggctacag atcagaaatg ctccgcctat tgcgcgcggc tgaggcattg
     7681 aaacgctgat tgtggacgga acttcgcgga tagctccgcg aagttcttct acctgagaga
     7741 attagagaat gaactgaagt agcagaacca cagccaccag aggagatact accattacaa
     7801 agaacttgtg gagcagtctt gcacctttgt cgttcttctt tgccatgagc cagataaccc
     7861 cggaaaggac ggcgatcaat cccatcacat agagaaaagc agtcagatag aaatagtccg
     7921 atcgcataat gtaggctacg gatggcgttt gcgaagcaag tgccattctg gacagtacaa
     7981 cggtgagaag tgcagtgagt cccagaggaa gacgtccttt cagttcttct atcggtgcgg
     8041 agattacaaa aaaggatgca acgagaatca cgaccagagg aacaaacatc gtgaaggcga
     8101 agccggttgg cactcgctgc acatccaggc gaaaccctat agtagaaaat agaatccgac
     8161 ttttgtatcc agcgcccatc ctgtaatccg ggtccccgaa agtggaatca tatctcaaaa
     8221 ggccagtgta gttcttcttc tgtatgaacg tccaatcgag cggcaggcct ttcagaggat
     8281 cgtgagtcat gtgtcttgta tcagggacaa tgaatagatg tgcggaatgc tttactttgt
     8341 ttacgaaaga catttccagt ccctgtctat cgaatggaaa ctgactgaag tccatttgag
     8401 tgttgaaagt gcctttgcat ctgagcgcgc gataagtgac acctttcttt gtcctttctt
     8461 tgagaagcgt agtttttaga tcacccgcgc cgttgactgc gtcaatctga gaaggatcca
     8521 ctgctttgct ggcccacttg taccacatga agaactctac ttcgaagcgc atatccttcg
     8581 tgttgacgga gttgagcttc aaaatgtcca gacccatgaa aattacgtct acaacatgat
     8641 acggtgttcc tgccagatca atgacggagc cattggcgat ccgctccggc aactgtgcga
     8701 tcacgtaagg ctctttgact gtggccagtt gagtgaacgc aactttgtat cttccgtgct
     8761 ccactttcgc tacaaaaaga tctctatccg caaaacggcc ctcaccaaac accagttctg
     8821 cggttgctcc tacgagcaga tcttttcttt ttgcctgcag atcctttctc ttttgttcca
     8881 gcagatattc gtgaactagc ttacgatctg ttcctttttc tgcaatcgcc gtggtcaaaa
     8941 gcatggcggc gtcaaaagcc atggctccac ccaggcccgg ctgcactttg tattttgcct
     9001 cgtaggcttc gcgcatggca gatgcagttt ggtttgcaat ttcccatagg aagggagtgc
     9061 aaacgtaaag agtgtccgta taacgttccg cttctttggt tgtgtacttt ggattcaagt
     9121 aactttcagg cgcaagcatc acagtgtctt ctggagccac tttgctattg tctatgattg
     9181 tgagaagctt gaggcccacg tccgcattgt cgtctgtaag aagaatgatg gaatctgcag
     9241 ttggatcctg ggtgagattg gccgcaaatg cttctgtacc cagatcttcg ttcttaaagc
     9301 gaatgacttt gacgggcgta atggaaagtt cgcttgcgcg ctcttcaaat cctttccaat
     9361 gcctgagccc gtagcgcgat tcctgtacaa atacgattgg atgcgatttg cccatggctc
     9421 ctttcagata gtccaccatg aattgtccct ggtaaacacc atcgcgattg agcatgaata
     9481 tgttcttagt tttagtgctt agatccgtgt ttgtactaac gggagtgatg gccaccagat
     9541 ccgtcttgtc gtaaacttcc gccgctgcga tgacagtggg cgaattcaaa tggccaatta
     9601 ccccaactac attcgagttt cccgcaagtt cttccgcggc cttcttcacc ttcgcaggat
     9661 cgtcttcgtc gtccttgatg accaatccaa ctttctttcc gccaattccg cctttttcgt
     9721 tgatagaatc cacgtgcagc tgtactgcct ggagcatttc ttttccagtc tctgcatttg
     9781 gtccgctgag aggtaatgaa acgccgatga gtattttttt atcgcatcca atggtgagtg
     9841 caaggatgag gagtggaatg agccggccga tatttcgcat ggttttttgt gagaaacggt
     9901 agtgtggcag aacgtcaagc gcaaccatat tccttcttgt ttttttagca tccctggaca
     9961 ggatgagagc catgagactg gcgttctgtt ttgctttctt gatatcctct tctcttctgg
    10021 cggacgaaag ccttcatccc ggtcctgcat ttgtgtttgg aacccaggtc aacgtgcgag
    10081 agaaaggcgc aacgactgct gccgttaagt tcatgttgcc gattggtgcg aaggtgacca
    10141 ttgagactgc aacgcaggaa aaggtgatcc agggcgccag aagtgatttc tggtatcaaa
    10201 tcaaaactgc agacgggcga aacggttttg tatttggatc tcttctgacc gcgcttggcg
    10261 aagaactcgg cgacggtcgc ctgcttcttg ttggctgggc ggagaatgcg aaggactcgt
    10321 ttgagtttcg cattgtacaa aaaggaagtc tggtttcatc acgagcgttc gcgctccttg
    10381 gttactctca agatccagct gccgccaatt actgtcatac catggagttg catcgcaatc
    10441 tattcggtca gggcaatcat gcgatttctc tcttgagttt tgcaggctct gagatggacg
    10501 ctggattctc aaacggggct tacgcattca ttctgcgcgg aaatacaatc gacctggttt
    10561 cgcagggtca gggaggagcg gatgctcctg cgtatgcgga ctatacgttt tctttccgtc
    10621 ctggaatcat tgaagaaaaa tttgaagagg tgggagacat acaggatgga atcgaaatcg
    10681 gggaaggata cacgcgtcag ttcaagctcg tggcgggaaa gattgcggct tctgaaaaac
    10741 agatctacaa aattcgcaga ccagttcccc agtaactatt tctttctcac tggcattaga
    10801 tccgtgcagc ttccgtgagc cacttccgca gttagtccaa cagtttctcc cagagttgga
    10861 tgcggatgga tggttttgcc aatgtctact gaatcgcatc ccatttcgat ggcgaggcac
    10921 acttctccaa tc
//
LOCUS       DAORFF010000039        10714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2805_length_10714_cov_3.964256, whole genome shotgun sequence.
ACCESSION   DAORFF010000039 DAORFF010000000
VERSION     DAORFF010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10714
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2805_length_10714_cov_3.964256"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PKK76_02855"
     CDS             <1..262
                     /locus_tag="PKK76_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67432.1"
                     /translation="LFDGGRIHRISPLKDFKEHLVDLARDLSLRANVAYVAGPVRLSS
                     GKQLNPASVEFIMNWWIAAAILAGAFAGIAPRILIELVSKYV"
     gene            1128..1595
                     /locus_tag="PKK76_02860"
     CDS             1128..1595
                     /locus_tag="PKK76_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67433.1"
                     /translation="MKLSIAVILSMLSFACKDTRPIRQIELSKFKCDKSAKELSDQPV
                     AISGYLVPPVVIFCKSGKCSVNLQLTEEQLEPSVTIDLVPGSGPNTMDMPPENFRKTD
                     FAFRDAAGKGHRSNEQVVVTGIFDFTDAYETIPNGCRIRQVREIRPEGQFLAK"
     gene            1592..1837
                     /locus_tag="PKK76_02865"
     CDS             1592..1837
                     /locus_tag="PKK76_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67434.1"
                     /translation="MKKQQIPGQSPHQIPPELIEAARNNPNSFVYKIDGSYPIRDPLG
                     DALPASAIVGAWKVDGNGNITGDFVPNPNYRPWKPDA"
     gene            2015..2317
                     /locus_tag="PKK76_02870"
     CDS             2015..2317
                     /locus_tag="PKK76_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67435.1"
                     /translation="MREYVPSFQRRATIRFFLKGDYTAEGAESTNVFLAADGKFSLFL
                     GFLLLAFGLFICAVGNSPVILILIPVALLYWPVTAVRFRNSIQRQVQAVRAALEAR"
     gene            2471..3031
                     /locus_tag="PKK76_02875"
     CDS             2471..3031
                     /locus_tag="PKK76_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67436.1"
                     /translation="MEHFDKRTPIPDSARRAATVLEALLIRALAIWTMLAQLCMGLNC
                     IGLGAGFFESTERFHSGPDSNPITSSELRDREGAPDAIVKTSSETEEWYYYYYKPLGF
                     DELRTPIGYRWWGPYLHAGCIGIPLVVPIGRGYRKYVIRKQQTEASYSTDTNLCFAGC
                     MCTFVPHNDKCECSFGCDSNFANMLR"
     gene            3071..3649
                     /locus_tag="PKK76_02880"
     CDS             3071..3649
                     /locus_tag="PKK76_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67437.1"
                     /translation="MRRILPRLIEMLLQNAPVLFLAFASCKQDKIPAIEKIPGKPIRE
                     QTFTFQDSLLGPVTFVPVKDESAPPHEQLKIYLKLGDGSHTKLPDADINFIFHDMDAV
                     SFVDVDENGLTDIIIISQYISGIGRDAARPFRMAIIYLRRTDGFEFDFEDSWRVSELA
                     KGNLNIARVRTLFREVTRHAAKDGGTGKVRRP"
     gene            complement(3678..5135)
                     /locus_tag="PKK76_02885"
     CDS             complement(3678..5135)
                     /locus_tag="PKK76_02885"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNL67438.1"
                     /translation="MSEVCLNGPFDPAKVESECTWKETQWAQESFGYVEGPVWVRFHV
                     RNRTAPTHWLLISEYPWIDHVDLFEHRDGNLRLVEESGDSVSVTRASVPAKQPTFALE
                     LPSNQERVYYVRFVGNAEIYIGLTLEDNLTFGTRSFSVRDLFVAFVGMSLLVFVANSF
                     VWFRLRAPLSLYYCLFNVSSLAYGCLLDGSFLRMALKTTRFSDDQLHFGIGLFAAGSI
                     LLLWTRLIPMSDLFPRLNKFSRWYAIANCAAGLLTVIGVFPHLVVDVLARILMMYSNL
                     LFYPTAIIAYRRGFRPALYPIFGMPVFSVFMFCYLMSGVVFSGNLIWKNSVWYAYLVE
                     YSVFFASMVSRIRFETSGQMETFRESLARKSRLTGLDIEQLRLRLEEQLSQGVYEDET
                     LSIAKLALLLKIRPDQLSELINVLYGMGFRRLINSRRVMKAQELLETQLDRTVLAIAL
                     DTGFGTKAAFNREFKLQTGLTPTEYRRRNSTLRRP"
     gene            5616..5885
                     /locus_tag="PKK76_02890"
     CDS             5616..5885
                     /locus_tag="PKK76_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67439.1"
                     /translation="MKRLLLSITLCMSSTIACTTTSGLLDPRGSSDGKRITARKSELT
                     FYCFWASLHWIDVECEVSASNILQCRKMNPSFQLRDAIIVEGKKL"
     gene            6043..6657
                     /locus_tag="PKK76_02895"
     CDS             6043..6657
                     /locus_tag="PKK76_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67440.1"
                     /translation="MQESQRKIPRIGKWLTIVVLTSTMPSCITWYTGKPDQTTTLDNK
                     EEFHFSVTSYGEAGVGAIAKADVAVAEMLRKFGYTRAVQEKSRLDYQNKGGRDPFIEI
                     ACSQSHVVRWQSASAYWAVASLGTLGVIPHFGSQDLQVDVALYHFDATKGSFVAKRIR
                     SYNMKGYRFSGILALPVFWVSWIADGEKEVVRRAVLDFLQEPLI"
     gene            6654..7235
                     /locus_tag="PKK76_02900"
     CDS             6654..7235
                     /locus_tag="PKK76_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67441.1"
                     /translation="MKFASLLLLFLLSCTTYYAGVKAPAEDAKLLQIAYRKPPLLIPK
                     VNGVSYGFLLNPIFFTQPGRNDLDVLYSVVTGANSSIGSRETIPVSVEGAGGTSIVVC
                     DQTAPDMSSFSVHFYSVPFHLTDDHEGKRLLDAACEVFDWVTAGRNLDVARALKWGLP
                     LSMRSHRGATLADHIRKEAQDKKNPDLLKLIEK"
     gene            complement(7253..8602)
                     /locus_tag="PKK76_02905"
     CDS             complement(7253..8602)
                     /locus_tag="PKK76_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNL67442.1"
                     /translation="MIYRTQNPHTEIFEKLYDISSETTTDTTLRSAESASLVWSAMSF
                     SERAVRIRALADGLLRRKDEFAAVMAREMGKPIGQGLAEIEKSAVLIEFYAAKAESFL
                     SPEIIPSESSKSYACFDSLGVVFGIMPWNFPFWQVLRFAVPAWMAGNAAVFKPAPIVP
                     ECAVLLTSLCREFEVPLWHLFLEEKATAQVMTDPRISVISFTGSVRTGKLIAARAGLN
                     MKKGVYELGGSDPYLVLEDADVSRAASICASSRLINSGQSCVAAKRFLVHRSLKKSFE
                     EAFVAAMNVSYGDPLTNPALGPLARPDIRIHLADQVRSSVSQGAKLLLGGEIPDQKGY
                     YFPPTVLTDLSTEMAVWDEETFGPVAALHYFDSDEQAIALANDSDFGLAAAVFTADSA
                     RGESVALKLKVGSVSVNDLTRSDPRFPFGGIKDSGMGRELGSFGIREFTNIKTVKIA"
     gene            complement(8720..9106)
                     /locus_tag="PKK76_02910"
     CDS             complement(8720..9106)
                     /locus_tag="PKK76_02910"
                     /inference="COORDINATES: protein motif:HMM:NF019473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNL67443.1"
                     /translation="MLEENKNLVREFFEAINRQDLARAEGMLSEQLSWWIIGRAKISG
                     QKDRRSVRISFKMVFRGFDHFAFTLHDITAEGNRVAVTAESRGRHKSGKDYNNHYHFL
                     FFIEDSQIMQVKEYFDTEHAMWLEAG"
     gene            complement(9180..9605)
                     /locus_tag="PKK76_02915"
     CDS             complement(9180..9605)
                     /locus_tag="PKK76_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNL67444.1"
                     /translation="MDSRRIIHLLSRTRDRIQKHFQHEIDGLKVGKIAPAHGSVLYAL
                     MDGPKAMQDLARLIERDTSTVTALADKLEKVGLAQRTVAPHDTRSYFMALTPKGKIAA
                     RKVIGASRGVVTRIFKGFSAAEREEFTRLLFRVYQNMEE"
     gene            complement(9673..>10714)
                     /locus_tag="PKK76_02920"
     CDS             complement(9673..>10714)
                     /locus_tag="PKK76_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67445.1"
                     /translation="FSLTLTACQGPTRLAEHWEVGELSVDGRTVFGSTLDGIRSVGRD
                     GSRGTLLVPYRTSVFEESQYRPGRIAVDDSYVYWTQREAPPGLDGVRRVKKTGGSPES
                     VMKVNSLAQSQPLHLQLDHAFVFVLTYGRGQFDNWIADTAGTYEGGSLVRIDKKDLSV
                     MDLASRVRAGSGLCVDSQFVYWSEGGTFHRTISGLADHVYNMDGKIYRVRKNGGKPEL
                     LASGLDNPTELSISGGNIRFRSGRHYQKNSWTDDLGYFEIPTSGGDAKKIAPYPYFEM
                     GQNRYWLRIEYFGPANPGRRAHHFLEKESAAGKIERLYSTDDDISAFTLDGGEFFWLQ
                     GTDYTLMKMKVR"
BASE COUNT         2749 a         2794 c         2619 g         2552 t
ORIGIN      
        1 cctttttgat ggagggcgca tccatcgcat cagtcccctg aaggatttca aggaacacct
       61 ggtcgatctt gcgcgcgatt tgtctctgcg cgcgaatgtt gcctatgtgg caggcccggt
      121 ccgattgtca tccggtaaac aactgaaccc tgcaagtgtc gagttcatca tgaactggtg
      181 gattgccgcc gcgatcctcg ccggtgcctt cgcagggatt gcgccaagaa tccttataga
      241 gcttgtttcg aaatatgtat agtcgagcgg agaacagacg aaaaaagaga ggatcatggc
      301 aggttgtgag gaatagcatt tcaaaaacaa aaacctggaa tctgataaac gggtccgctc
      361 caggtttgtg ggcgattgcg acattcttgt ttgtatgcaa attgcttaac agccatcgcg
      421 aaaaaccgcc tggggccaaa aacgattgag accaccagaa agccgacatt gattgtctca
      481 atcgaggagt cggagcattt tctgcgagtc gtctggcaag gagaggcaga ccatcaaagc
      541 tcgtattggc caagcggccc ctgcagatgc tagtgacatc cttacgcaaa aggaagcttg
      601 ctttcgtcgt aaggaactgg caacaaacat cacgattgaa tccggggaat aagtcgcttg
      661 tccgaaggga gaaaccttca tggagccaaa cggggaaatc ctggcgaagc agaagcaggc
      721 gctggtcttc ttgccggatt caggacccgg tgaccggagc aacgagattt ccagagcgga
      781 aatcacggaa ctgaaatggc gcgggaggaa cgggtctcct gagctgacga tatccttagg
      841 aatccttcaa cccgagcgtg aaactagaat cggccatgca tatttcctct atcctttaac
      901 ggttccagtt gatattgtaa ctgccccgtt tcagctgctc tattttgggc ctttcaacta
      961 cttgttgatc ctttactgca cgattggctg gtcctgcaaa aacgggcttg gctgaaatga
     1021 accgaaggaa ccattgcact ttccagagaa tcgcgactca tgcgcaaatg gcggatccat
     1081 ccgccaattc catttgactg accaaagaat actcagcctg cttggctatg aaactttcga
     1141 tcgcggtgat tctttcaatg ctgtcatttg cgtgtaaaga caccaggccc atcagacaaa
     1201 tcgagctctc caaattcaag tgtgataagt ctgcaaaaga actctcagat caaccagtag
     1261 caatttctgg ctatctcgta ccgcctgtgg ttattttttg caagtctggc aaatgctctg
     1321 tgaacctgca actgacagag gagcagctcg aaccctctgt tactatagac cttgtgcccg
     1381 gaagcggacc taacacaatg gatatgcctc ctgagaattt cagaaaaaca gacttcgcgt
     1441 tcagggacgc tgcagggaaa ggccatagat cgaacgaaca agtagttgta acgggaattt
     1501 ttgatttcac ggacgcctac gagacgatcc cgaacgggtg caggataagg caagtacgcg
     1561 aaatacgccc tgaaggacaa tttcttgcga aatgaaaaaa caacagattc cgggccaaag
     1621 ccctcatcaa ataccgccgg agctgatcga agctgcacgc aacaatccaa acagcttcgt
     1681 ttacaaaatt gatggttcct atcctatccg tgatccgtta ggcgacgctc taccagcatc
     1741 tgccattgtg ggggcatgga aagtagatgg taatggaaac atcaccggcg atttcgttcc
     1801 caatcccaac tacagaccat ggaagccaga tgcgtagccc gggcacatga aagaattaca
     1861 gcaacatttt tggaaaatga gcatagggtt ttccttccgg gtacccctcc gtatagatca
     1921 ggtagagatc cgaattcaca aattacttac tccagaaaag ggatgggttt ctggacgcgg
     1981 gtttgaaggt gagtgcagtg gaggcagttt ctggatgcga gaatacgtcc cttccttcca
     2041 gaggcgcgca accattcgct tctttctaaa aggagactac acggcggagg gcgctgaatc
     2101 cacgaacgtg ttcttagctg cagacgggaa gttctccctc tttcttggct tcttgttgct
     2161 ggcatttggt ttgtttatct gcgccgtagg aaactccccg gttatcttga ttctaatccc
     2221 ggttgcgctg ctctactggc ccgtaactgc tgtgcggttc aggaatagca tccaacgaca
     2281 agttcaagca gtccgcgccg cactggaagc gagatgaaat ccaacgacga aacgttgata
     2341 tcatgaatga ttttgcagcc aacgtggccg ggctacttgg tcgcataggc gcaatttgct
     2401 ttgccgcctc aatgcttgta ggcactttct acttgttcta cgttacgatt cgtgcctatt
     2461 tgcggtttgc atggaacatt ttgataagcg tactcccata cctgattctg ctcgtcgcgc
     2521 tgcaactgta ctggaggcat tgttgattcg cgcactcgcc atatggacca tgctcgcgca
     2581 attgtgcatg ggcctgaatt gcatcgggct cggtgccggt ttcttcgaat ctaccgagcg
     2641 ctttcattct gggccagact caaatccaat tacgtccagc gaactgaggg accgtgaggg
     2701 cgcaccagat gcaattgtaa aaaccagttc tgagactgag gagtggtact actattacta
     2761 caagcccctc ggctttgacg agctaaggac tccgattggc taccgatggt gggggccgta
     2821 tctgcatgct ggctgcatcg gtattccact tgtagttccc attgggcgtg gctaccgcaa
     2881 gtatgtaatc cgaaaacaac agacagaagc ttcctattcc acggatacca atctttgttt
     2941 tgcgggatgc atgtgcacgt tcgtcccgca caatgacaaa tgtgagtgct ctttcggttg
     3001 tgactcaaat ttcgcgaaca tgctacggta ggaaagagcg cgagtcacga agaatcaggg
     3061 gaatactgac atgagaagaa tcttaccgag actaatcgaa atgcttcttc agaatgctcc
     3121 agtcctcttc ctggcgtttg catcctgcaa acaagacaag attcctgcca ttgaaaaaat
     3181 ccctggcaaa ccgatccgtg aacagacttt cacttttcag gattcattgc tgggaccggt
     3241 cacttttgtt cctgtaaaag atgaatcggc tcctccgcac gaacaactca agatctactt
     3301 gaaactcggc gacggctcgc atacaaaact ccctgacgct gatatcaact tcatcttcca
     3361 cgatatggat gccgtatcct ttgtggacgt ggacgaaaat ggtctgacag acattatcat
     3421 catatctcag tacatatcag gtattggacg cgatgcagcc cgaccctttc gaatggcgat
     3481 catatatctt agacgcacgg atggcttcga gtttgatttc gaagatagct ggagggtttc
     3541 agagcttgcg aaagggaact taaatatcgc ccgagtacga actcttttca gagaggtgac
     3601 caggcatgcc gcaaaagatg gagggactgg gaaagttagg cggccttgaa agtgcgtccg
     3661 aaattcccaa catggtgtca gggccgccgg agagtactgt ttcgcctacg gtactctgtt
     3721 ggcgtgagcc cggtctgcaa tttgaattcg cgattgaaag cggccttcgt tccgaatcca
     3781 gtatccagcg caatggcaag gacagtgcga tcgagctggg tctcaaggag ttcctgggcc
     3841 ttcattactc gtctagaatt gatcaggcgg cggaagccca tgccgtacag aacattgatc
     3901 aactcagaca attgatcagg acgaattttc agaagcaatg cgagtttcgc aatgctcaaa
     3961 gtctcgtcct cataaactcc ctggctcagt tgttcttcca atcgcaatcg cagttgctca
     4021 atgtccagtc ccgtgagtct tgatttccgt gcgagtgact cgcgaaaggt ctccatttga
     4081 cccgacgttt caaatcggat tctggaaacc atggaagcga agaatacgga atactcaacg
     4141 aggtatgcgt accatacgga gttcttccaa atgaggttgc cagaaaaaac aacgccggac
     4201 atcagataac aaaacatgaa caccgaaaaa accggcatgc cgaagatagg atacagggcg
     4261 ggtcgaaacc cacgcctgta tgcgatgatt gccgtcggat agaatagaag attcgaatac
     4321 atcatcaaaa tccgcgccag aacatcaact accaggtgcg gaaagactcc gatgacggtg
     4381 agcaaacctg cggcgcaatt ggcaattgca taccaacgac tgaacttgtt caggcgcgga
     4441 aagagatcag acatcgggat gagccttgtc cacaaaagca gaatgcttcc tgccgcgaag
     4501 agtccgattc cgaaatggag ctgatcatca gagaatctag tcgtcttcaa cgccattctg
     4561 aggaacgaac cgtcgagcag gcacccatag gcgagtgacg aaacattgaa gagacagtag
     4621 tagagagaaa gaggagctct cagccgaaac cagacaaaac tatttgcaac aaagacaagc
     4681 agactcatcc caacgaaggc tacaaataga tcgcgaacag aaaaggatcg cgtcccaaag
     4741 gtcaaattgt cttcgagtgt caggccaata tagatctcag cattgccaac gaaccgaaca
     4801 taatacactc tttcctgatt tgatggcagc tcaagtgcaa aggtgggctg tttggcggga
     4861 acacttgcac gggtaaccga tactgaatct ccgctctctt ccacgagtcg caaattaccg
     4921 tcacggtgct cgaacaaatc gacgtgatca atccacggat attccgaaat caggagccag
     4981 tgagtgggag ctgtacgatt tcggacatgg aaacgtaccc atactggccc ttccacatac
     5041 ccaaagcttt cctgcgccca ttgggtctct ttccaggtgc attcactttc taccttcgcc
     5101 ggatcgaaag gcccattcag gcacacctcg ctcatcgaga gtatatcctc acggataccc
     5161 tgaacggtca aatcgactac cctttccgca aaagctggag actgaagtgc gcagcaaacc
     5221 acgaagcaac agacaaatct aaagatcatt cgttcctacg cggagagaat tccgtctcct
     5281 tcacttttct gcaggaatat tttctaaccg cgaccacaat catgaatcaa gtcgacaagc
     5341 acctatgtcc aggactaaaa tgagcccatg ttgacaaatg ttggaaaact cttgttgccg
     5401 atttctgtgg cttgcttgag cagctgtcag agcgctacgc acgtatacag tgcagtaccc
     5461 ttcgcagaag actcaatctt gatctctgca atggcacagc cacgtccggg cagcggaaac
     5521 gcacttgagc cgtacattgc taggtgcaag attcaggcca accaggacct tgaatgcaca
     5581 aagatgatgg tcgaatggcc ggatggagaa ggtcaatgaa gagactactc ttatcaatca
     5641 cactctgtat gagctctacg atcgcatgca caactacgtc aggcctcctg gatccacggg
     5701 gatcgtccga cggaaagcgc attacggcca gaaaatccga actcacattc tactgtttct
     5761 gggccagcct tcattggatc gacgttgaat gcgaagtgag tgcatcgaat atcctgcaat
     5821 gccggaaaat gaatccatct ttccagcttc gggatgcgat cattgtggag ggcaagaagc
     5881 tatgaaagca gccatgttcc tactgctggc agtgtgcgcg tgcaaatcgt tcgaaaccgt
     5941 tgttctggta ccaacgaacc agggaactta tcggattgct cgcattgact gggccatttc
     6001 ggacgaagca gagttttgca agcgcgagga gggaagaata aaatgcaaga aagtcaacgt
     6061 aagattcccc gaataggaaa gtggctcact atcgtagtat taacatcgac gatgccgagt
     6121 tgtatcacgt ggtacacagg aaaaccagac cagacaacca cccttgataa caaagaggaa
     6181 tttcactttt ccgttacgtc atacggcgaa gctggagtcg gagcaatagc aaaagcagac
     6241 gtagccgtgg ccgagatgtt gcggaaattt ggctacaccc gggccgtgca ggaaaagtcg
     6301 cgactggatt atcagaataa gggcggacga gatcctttta ttgagattgc gtgttcgcag
     6361 tcccatgtag tgcggtggca gagcgcctct gcctactggg cagttgcttc cctgggaacg
     6421 ctgggagtca tcccccattt cgggtcgcag gatctacaag tcgatgtggc tctctatcac
     6481 ttcgatgcga caaagggatc gttcgtcgca aaaagaatcc gctcttacaa catgaaaggc
     6541 taccgcttct ccggcatcct cgctctcccc gttttctggg tgtcctggat cgcggatggc
     6601 gaaaaggagg tcgttcggcg cgcagttctt gattttctac aggagccctt aatttgaaat
     6661 ttgcatcttt gcttctactt tttcttctct cttgcacaac ttactacgcg ggagtgaagg
     6721 ctccagcaga agacgcaaag cttctgcaga tcgcctaccg caagccgcct ttactgattc
     6781 caaaggtgaa cggcgtctcg tacggtttcc tcctgaaccc gatctttttt acgcagccgg
     6841 gtcgcaacga tctggacgta ctctactcag tagtcacagg agcaaattcc tcaatagggt
     6901 cgcgcgaaac tattcccgtg agcgtagagg gtgccggtgg gacttccatt gttgtgtgcg
     6961 atcaaactgc tccagacatg agctcattta gcgttcactt ctattctgtt ccatttcacc
     7021 tcacagatga tcacgaaggg aagcgactgc tggacgcggc ttgcgaggtg tttgattggg
     7081 ttacagctgg acgaaacctg gacgtagctc gcgcactgaa gtggggattg ccactctcca
     7141 tgcgcagcca tcgcggagca actctggcgg atcacatacg aaaagaagcg caggacaaga
     7201 aaaatccaga tttgctgaag ctgattgaaa aatgattcag atcgcgcagg cgttaggcga
     7261 tcttgacagt cttgatgttt gtaaactctc tgataccaaa cgatcccagt tccctaccca
     7321 ttcccgaatc cttgattcct ccaaatggaa atcttggatc cgatcgtgtc agatcgttta
     7381 cggatacgct gcccaccttg agcttgagag ccacactctc gcctcttgca gagtctgcag
     7441 tgaaaacagc ggcggccagg ccaaaatcgc tgtcatttgc aagagcgatc gcctgctcgt
     7501 ctgaatcgaa atagtgaaga gcggccactg gcccaaatgt ttcttcgtcc cagaccgcca
     7561 tctccgtgga aagatctgtg agcactgtgg gcgggaaata ataccccttc tgatctggaa
     7621 tttctccacc taacagcagt ttcgcgcctt gagaaacgct cgaacgaacc tgatctgcca
     7681 ggtgtatgcg aatgtccggt ctggccagag gtccaagggc aggattggtc aaaggatctc
     7741 cataggaaac gttcatcgcg gccacgaatg cttcttcgaa agatttcttc aagctccgat
     7801 gcacgaggaa gcgcttggcg gcgacacaac tctggcctga attgatcagt cgactgctgg
     7861 cacagatgct cgctgcgcgc gatacatccg cgtcttcaag aaccagatac ggatcagacc
     7921 cgccaagttc gtaaacgccc ttcttcatgt tcagaccggc tcgcgcagca atcaacttcc
     7981 ctgtacgaac gctgcccgta aaagatatca cagaaatgcg cggatcggtc atcacctggg
     8041 ctgttgcttt ttcttcgagg aaaagatgcc agagcggaac ttcaaactcg cggcaaagcg
     8101 atgtgagaag aaccgcgcac tctgggacaa tcggagcggg cttgaacaca gccgcattcc
     8161 ccgccatcca ggcaggaaca gcaaacctca gaacctgcca gaaaggaaaa ttccagggca
     8221 tgattccaaa gacaactccc agagaatcga aacaagcata actcttagaa gactcgctcg
     8281 ggatgatttc aggagaaaga aatgattctg ctttcgcggc ataaaactca atgagaaccg
     8341 ctgacttttc tatttcagcg agtccctggc caatgggctt gcccatttcg cgcgccatca
     8401 ctgctgcgaa ttcatctttt ctgcgcaata gtccatctgc gagagcccgg atgcgtacag
     8461 cgcgttctga aaacgacatc gcagaccaga caagggaagc gctttcagcg gagcgcagag
     8521 tggtgtctgt cgtagtttca gagctgatgt cgtatagttt ttcgaaaatt tcagtgtgcg
     8581 gattctgagt ccgataaatc atccctcgtc cttccatcgc aagtgcgata ctgccgtttt
     8641 gccgctgttg cagatgccgt cagctgcatt ttgaatcatc gcatgcgtag cgttacgttg
     8701 atgtcacgcc gtcaacagct tagccggcct caagccacat cgcatgctcg gtgtcgaagt
     8761 actctttgac ttgcataatc tgtgagtcct cgatgaagaa aagaaaatga taatgattgt
     8821 tgtagtcttt cccgcttttg tggcgccccc gcgattcagc ggtcaccgcc acccggttgc
     8881 cctccgctgt gatgtcatgc aacgtgaaag caaaatgatc aaaacccctg aagaccattt
     8941 tgaacgaaat gcgaactgaa cgacgatcct tttgccccga tatctttgcc cttccaatga
     9001 tccaccagct gagctgttcc gaaagcatcc cttctgccct cgccaggtct tgccgattga
     9061 tcgcctcaaa aaactcgcga accagattct tattttcttc cagcatattc atctcctttg
     9121 ttccgctctt ttcgaatcgg aatctatttt attttgacat caaactaatg cataaaaaat
     9181 tactcctcca tattctgata aacccggaac agcaggcggg taaactcttc gcgctccgct
     9241 gcagaaaaac ctttgaagat cctggtcacg actccgcgcg aggctccaat gaccttacgc
     9301 gccgcgatct ttccttttgg cgtgagtgcc ataaagtagg aacgagtatc gtgcggcgca
     9361 accgttcgct gcgccagtcc aactttctcg agcttatctg ccagggccgt tactgttgac
     9421 gtgtctcgct ctatcaggcg cgcaagatcc tgcattgcct tcgggccgtc catgagcgca
     9481 tacaaaaccg agccgtgggc gggcgcaatt ttgcccactt tcaacccatc gatttcatgc
     9541 tgaaaatgct tctgaattcg gtctctcgta cgcgaaagca gatgaatgat tctcctggaa
     9601 tccatttact ttgacatcaa agtaaatacc cgggctatgt caagatgatt tcaaacccgc
     9661 aatgatcgct tgttaccgaa ccttcatctt catgagtgtg tagtcggtgc cctgcaacca
     9721 gaagaattcc ccaccatcga gtgtgaatgc ggagatgtca tcgtctgttg agtaaagcct
     9781 ttcgatcttg cccgccgcgc tctctttttc gagaaaatgg tgagccctcc gaccgggatt
     9841 ggccggtcca aaatattcaa tccgcaacca gtatctgttc tgacccatct caaagtaagg
     9901 atacggcgct attttctttg catcgccgcc tgacgttgga atttcaaaat atccgagatc
     9961 atccgtccag ctgttcttct ggtagtgtcg tccgcttcta aaacgaatgt ttccgccgct
    10021 tatactcagt tcggtagggt tgtccaaacc ggacgcgagc aactcaggtt tcccaccgtt
    10081 ttttcttact ctgtagattt tgccgtccat gttatacaca tggtccgcga gaccggagat
    10141 ggttctatga aacgttccac cttctgacca gtatacgaac tgcgaatcca cacatagtcc
    10201 agatccggcg cgcacacgcg aagcgagatc catcacggac agatccttct tgtcgattcg
    10261 aaccagagat ccgccttcgt aggtcccggc agtatctgca atccagttgt caaactgacc
    10321 gcggccatac gtcaggacaa acacgaacgc atggtctagc tgcaagtgaa gcggttgaga
    10381 ttgcgcgagg ctgtttactt tcatcacact ctctggagat ccgcctgttt tcttcacccg
    10441 gcgcacacca tccaacccgg gtggtgcctc ccgctgtgtc caatagacat atgaatcatc
    10501 caccgcgatt cgccccggcc gatattggct ctcttcaaac accgaagtgc gatacggcac
    10561 aaggagcgta ccccgcgagc catcccgacc gacgctgcga atcccatcga gtgtggatcc
    10621 gaatacagtt cttccatcca cgctcagttc accaacttcc cagtgttccg ccagcctggt
    10681 tggcccctga cacgcggtca gcgtaaggga aaaa
//
LOCUS       DAORFF010000040        10699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2812_length_10699_cov_3.538144, whole genome shotgun sequence.
ACCESSION   DAORFF010000040 DAORFF010000000
VERSION     DAORFF010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10699
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2812_length_10699_cov_3.538144"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PKK76_02925"
     CDS             <1..583
                     /locus_tag="PKK76_02925"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNL67446.1"
                     /translation="VGRMRGIDVMTQGSCNPGASNVYRLAGPIPGILVFLLDVAKGAL
                     PALAGMYLAVHDLSGNATEPNPVLQIWLELGAGFSAAFGHIFSPFLKFKGGKGVATFL
                     GVFLVIFPAGILTACALAAVAILITRYFSVGSILGAIVLPVSYFIFVDPVWAPQNVPV
                     LCLTILASVVILIRHRENISRLIKGTEHKTRHV"
     gene            576..1157
                     /locus_tag="PKK76_02930"
     CDS             576..1157
                     /locus_tag="PKK76_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67447.1"
                     /translation="MFEHLQEISERASIESMTRTAEQFLDNHPIARARDLYRWLWEGE
                     FGPGTRHQPTRLDELTQMIRTARMHKSAGSQPMWEPLGLTCRFVHVNLIPYSDAGCPL
                     KRILTLGERTRDYRADQLRFKMQWAFMKTQIIHGMRITVEEMVSFENDIAFHMTPEIS
                     FTDEYLAEYGSGYMVVPYDLFFETYPEYAEHES"
     gene            1157..2152
                     /gene="mtnA"
                     /locus_tag="PKK76_02935"
     CDS             1157..2152
                     /gene="mtnA"
                     /locus_tag="PKK76_02935"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNL67448.1"
                     /translation="MRIPTGVTFEDGKLYYVDQTKLPGTFEKKECTTLEEAEEAIRQL
                     RVRGAPAIATFASFAAALEMDRKRHLAHPQWKVEAEQVIDRLLATRPTAVNLSHAMKR
                     LRERLAAGDRSLLSEAAAIQEEDKQLCDALGKSGSTLIPAGARVITHCNTGALATAGI
                     GTAIGSFYTAAAEGKDITVYACETRPLLQGARLTSWELDRAGIRTILITDSMAAFIMK
                     TEKIDLVMVGADRIAHNGDTANKIGTLGLAVLAQHFGIPFYVVAPSTSVDPNLASGDL
                     IHIEERSAAEVRTIAGISTSPEHIAVRNPAFDVTPAELIRAIVTEKQIHFPPYSF"
     gene            complement(2233..5832)
                     /locus_tag="PKK76_02940"
     CDS             complement(2233..5832)
                     /locus_tag="PKK76_02940"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNL67449.1"
                     /translation="MRINRYFSKAADTQSQISLLNAAHAGDRETDNPVWQQFTWTRKS
                     SKIVNPDGSVVFEAPAVEVPEAWSQVAVDILAQKYFRKAGVPVYTKRVAEEGIPAWLQ
                     RRTADEERLATLPEDKRYIGESSGKQVFERLAGCWTYWGYKYGYFETEADAFHFFQEH
                     CYMLAAQISAPNSPQWFNTGLHWAYGIDGKSQGHYYVELKTGDLVRSQSSYERPQPHA
                     CFIQSISDDLVNEGGIMDLWVREARLFKYGSGTGTNFSNLRGEGESLSGGGKSSGLMS
                     FLKIGDRAAGAIKSGGTTRRAAKMVCLDMDHPDIEEFISWKVIEEQKVLSMVAGSRLA
                     NRHLNHILSAIHAEENPENKFDRQKNAPLKKAIHEARLAQISENYIQRMIELASQGYK
                     SILFEELTTDWQSRAYQTVSGQNSNNSVRVTNEFMDAVESDASWNLYWRTERRRAQKQ
                     NREPKPSKTLRARDLWEQISYAAWSCADPGIQYDTTINEWHTCPEDGRINASNPCSEY
                     MFLDDTACNLASVNLLTLYNKDTGEFDDLGYRHTARLWTLVLEISVTMAQYPSQEIAR
                     LSHVFRTLGLGYANLGSLLMVMGHSYDSEKARAIAGALTAILHMSAYATSAEISERMG
                     PFEGYERNAKNMLRVIRNHRRAAYNAPASEYEGLSVFPVGISPVHCPDYLLKAAREES
                     DRALALGEKHGYRNAQVTVLAPTGTIGLVMDCDTTGIEPDFALVKFKKLAGGGYFKII
                     NQSIPPALKKLGYNAEQADAIVKYCVGHGTLKGAPEINPETLKAKGFTDEALARIDEA
                     LPSAFELGFLMNRFTLGLDFIKNTLGFGDDAEKPDFDLLTRLGFNKEQVRRANDYACG
                     TMTIEGAPHLAEEHYAVFDCANKCGRYGKRYLSVESHIRMMAAAQPFISGAISKTINM
                     PQEATVSDVKRAYRQSWELMNKAIALYRDGSKLSQPLNVQSDEEEDHSSTPHVQKVEK
                     IVEKIVYRTLTSRRRLPERRAGYTQKATVGGHKIYLRTGEYEDGHLGEIFLDMHKEGA
                     AFRSLMNSFAIAISLGLQYGVPLEEFVEAFVFTRFEPNGMVQGNRAIKMSTSIIDYVF
                     RELAITYLDRHDLAQVSPEDLRADSVGMGTEEGDSGKKTSEAADFDLPAFIGGREPSN
                     SRASAAVAEPSLGQLARLKGYEGDPCPECGQFTLVRNGSCLKCNSCGSTTGCS"
     gene            6077..7318
                     /gene="lysA"
                     /locus_tag="PKK76_02945"
     CDS             6077..7318
                     /gene="lysA"
                     /locus_tag="PKK76_02945"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNL67450.1"
                     /translation="MDSFAYKDSVLTWRGAEIRPLAAKFGTPLYVYSESALVERCQAL
                     GSAFGNRRAGIYYSVKACSNIHILRILAGLGCGADIVSGGELFRCRKADISPEKIVFS
                     GVGKTEAEIRYAITEGILFLSVESEAELHEISRIATITGKTARISVRVNPDVNPGTHP
                     YISTGLKENKFGISHKEVVRIYKLAQSLPGIEPTGLGFHIGSQITDLSAFADAGKIAV
                     RLVSEIEAAGIKLKHLDVGGGLGIRYANEEAPDPATYVRTLIQSVGLDHLELMFDPGR
                     SIAGNAGILVSKILYVKEGEKTFFIGDAGMNDLIRPSLYDAYHHVFSDPLRPGTRSGD
                     LVGPVCESGDFFAKDRELPAFESGDLAVLASAGAYGMSMASNYNSRPRAAEVLIARDG
                     TARLIRRRETFEDLLQPELDV"
     gene            7365..8384
                     /locus_tag="PKK76_02950"
     CDS             7365..8384
                     /locus_tag="PKK76_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67451.1"
                     /translation="MRTVILSLLLTLGGPLFAVSPDYSNMGQVLAQNRMYLLFMDTCV
                     VNLRGPSAQEDPKGMRAQFEGYYKAAIEHDFYANLWYLQGNYGKTYSELRLSQNALQD
                     LYRRILENYIDETVILLEASAPIIVLTRDQSAKKLLELGFRDLESTRQIYLRGANTNP
                     KMFLNQIMTYQDGILRARRGRRFAILALLEAKVPVPEKSKYQVVTLDDVKNMMEEGEK
                     QTKYVEILNLLVNMTGRNLVPKVIASDVLGKMISLNTLEVHQDNYGRMYSDRRSVFQL
                     ITVTLRSDEFHKSDSMPRRNTDNRNEVPASDSDPIKPEGTTTQPQPQAITPVTPAPAT
                     DPGKK"
     gene            complement(8388..8726)
                     /locus_tag="PKK76_02955"
     CDS             complement(8388..8726)
                     /locus_tag="PKK76_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67452.1"
                     /translation="MIQLLGTIVITAVVSSLFTLGLSYAFYILHLRDLLDRKLDEKLE
                     LAKSKVKEGLREEALDLMPKFRAGVREGFKEALNSAMGGQIIENMTKPVSSVVEASLS
                     FLIGRQEEER"
     gene            complement(8723..>10699)
                     /locus_tag="PKK76_02960"
     CDS             complement(8723..>10699)
                     /locus_tag="PKK76_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNL67453.1"
                     /translation="VHGPVLVFAGAGSGKTRVLTQRIQRMIESGVAAAHITAVTFTNK
                     SAREMKERLSKLPREKRRGLIVSTFHSLGARILRSHIEKLGYGNPFTILSEDDRLRVL
                     SEIYSRHKLDPSDAKKDGLLSWFSRAKNALANPARFFESNGLDEGVIDFYEEYEHALR
                     STNSLDFDDLILLPIRLFHKDQELLEKYRKERTHYLVDEFQDTNPMQYEFLRLLVLPH
                     RNIFAVGDDDQSIYAFRGSDISLILNFHKDFAGASVHRLEKNYRSQAEIVRAASAVIV
                     RNTSRAPKEIRSVRPPGEKILGIYGTDEEEEAALVAEEIRQRIVRERRKPEDFAVLVR
                     TNFQSRAFENAFRSAGIPHRVVGGYRFFDRREVRDVLSYLRIIANPRDDLSLIRILSR
                     PGNGLGEVSIARIREASRDTGLYEFLVRMQGEPGLVQLKREGSAFIAELMEIMERHRP
                     AFARGVTQAARALVYELKFDSQFRREGEEDSAVSMRMQNISELLGMMQSLETEARQEG
                     EDFTIFDFLKAVSLLTSDDEEESGGRAQIMTVHVSKGLEFPVVFLSGLMDGLFPPERS
                     LSESGDIEITVAEERRLFYVGMTRAMDVLYLTASRTKKKFGEIIDMEPSRFLSELPED
                     SLVWTRGDPSRKPDPENELTDLISALDSFRAGKP"
BASE COUNT         2573 a         2692 c         2697 g         2737 t
ORIGIN      
        1 cgtgggccgc atgcgcggga tagatgtaat gacgcaggga tcttgcaatc caggagcgtc
       61 taacgtctac agactggcag gtcccatccc tggaattcta gtgttcttgc ttgatgtggc
      121 caagggtgct cttcctgcgc ttgctggaat gtatctggct gtccacgatc tgagtggaaa
      181 tgcaaccgaa ccaaatcctg ttttgcaaat atggctggaa ctgggcgctg gattttctgc
      241 cgcgtttggc cacatatttt cacctttctt aaagttcaaa ggcggcaaag gagtggccac
      301 ctttcttgga gtgtttctgg tcatctttcc cgcaggaatt ctaaccgcat gcgctctggc
      361 ggccgtggcc atcctgatca cgcgctattt ttctgtaggc agtattctcg gggccatcgt
      421 gttaccggtc agttacttta tctttgtcga tcctgtgtgg gcaccgcaga acgttcccgt
      481 tctctgtttg accatacttg catctgtcgt tatactcatt cgccacagag agaatatttc
      541 caggctcatc aagggaacag aacacaagac tcgtcatgtt tgagcatcta caggagatca
      601 gtgaacgagc cagcatcgag tctatgactc gaactgcaga acaattcctg gacaatcatc
      661 ctatagcgcg ggcgcgagac ctgtatcgct ggctctggga aggcgagttt ggcccgggca
      721 ctcgtcatca gccaacgcga ctggatgaac ttacacaaat gattcggacc gctcgcatgc
      781 ataagtcagc gggttcgcag cccatgtggg agcctctggg cctgacgtgt cgtttcgtgc
      841 acgtgaactt gattccgtac agcgacgcag gttgcccgtt aaaaagaatt ctcactctgg
      901 gagagagaac acgcgactat cgtgcagacc agctcagatt caaaatgcag tgggcgttta
      961 tgaaaacgca gatcatccat ggaatgcgca taacggtaga agaaatggtg tcttttgaaa
     1021 acgacattgc atttcacatg actccggaga tttccttcac agatgaatac ctggcagaat
     1081 atggaagcgg atatatggtc gttccttacg atttgttttt tgaaacctat ccagaatatg
     1141 cggagcacga atcttgatgc gcattcctac gggcgtaacg tttgaagatg gaaaactcta
     1201 ctatgtagat cagacaaaac tgccgggaac cttcgagaag aaagaatgca ctactctcga
     1261 agaagcagaa gaagcaatcc gtcagctgcg cgttcgagga gcaccggcga tcgcgacgtt
     1321 cgcttccttt gccgcagccc tggaaatgga tcgcaagaga catctggccc atccgcaatg
     1381 gaaagtagaa gcagaacagg tcatagatcg gcttctggcc acaagaccaa ccgcagtcaa
     1441 tctatctcac gccatgaaaa gattgcgcga gcgactcgcc gcaggagaca ggagtcttct
     1501 ttcagaagcg gctgccatcc aggaggagga caaacaactc tgcgatgccc tgggtaaaag
     1561 tggaagcaca ctcattccag ctggagcgcg cgtcatcacc cattgcaata ctggagctct
     1621 ggcgaccgcc ggcattggaa ctgcaatcgg gtccttctac acagcggctg cggagggaaa
     1681 agatatcaca gtctacgcct gcgaaacccg tccgcttctt caaggagctc gtctcacatc
     1741 ctgggaactc gatcgggcgg ggattcgaac gatcctgatc acagatagca tggctgcctt
     1801 catcatgaag acagagaaga tcgatctggt catggtgggt gcagatagaa ttgcgcataa
     1861 cggagatacg gcgaacaaga ttggaaccct tggccttgcg gttctggcgc agcattttgg
     1921 aattcctttt tacgtggtgg ctccgtctac ttccgtagat ccgaatcttg cctcaggcga
     1981 tttaatacac attgaagaac gatctgccgc ggaagttcga acgatagccg gcatttcaac
     2041 gagtccagag cacattgcag ttcgcaatcc ggcgtttgac gtcacgcctg cggaactgat
     2101 ccgcgccatc gtcactgaaa aacaaatcca tttcccaccg tactctttct aggctatcac
     2161 tgacccggcg cgaaggcata aaaaaacccg gccccgatgc gaggccaggt tttcgcgtga
     2221 ttcccgccta tatcaggaac agcctgtggt ggagccgcag gagttgcatt tcagacaact
     2281 tccgtttctc accagggtga actgtccgca ctcagggcat ggatcgcctt cgtagccttt
     2341 cagccttgcg agttgtccaa gacttggttc tgccacggcg gcggatgccc tgctgttaga
     2401 cggctcacgg ccgccgatga aagcgggcag gtcaaaatcc gcggcctcac tcgtcttttt
     2461 tccggaatcg ccttcttctg ttcccattcc gaccgaatct gcgcgaagat cctccggaga
     2521 aacctgagcc agatcatgtc tgtccagata tgtgatggca agttcgcgga aaacgtagtc
     2581 aatgatggac gtagacatct tgatcgcgcg gtttccctgc accattccat ttggttcaaa
     2641 gcgagtgaac acaaacgctt ctacgaattc ttcgagaggc accccgtact gcagacccag
     2701 agaaatggcg attgcaaacg agttcatcaa tgaacggaat gctgcgccct ctttgtgcat
     2761 atccaggaat atttctccca ggtgaccgtc ttcgtattct ccagtgcgaa gatagatctt
     2821 gtggccaccc acagttgctt tttgggtgta gcccgctctt ctttctggaa ggcgtcttct
     2881 gcttgtgaga gtgcgatata caatcttctc cacaatcttt tctactttct gaacgtgagg
     2941 agtagaagag tgatcttctt cctcatcgct ttgaacgttg agaggttgag aaagtttgga
     3001 tccgtctcta tacagagcaa ttgctttgtt catcaattcc caggattgac gataggcgcg
     3061 cttgacgtcc gataccgttg cttcctgagg catgttgatt gttttggaaa tggcaccgga
     3121 aatgaaaggc tgggctgcag ccatcattct gatgtgagat tctacagaga gatatctttt
     3181 tccataccga ccacacttgt tggcgcaatc aaacactgca tagtgttcct ctgcaagatg
     3241 aggagcgcct tcgatggtca tcgtgccgca tgcgtagtca ttggcacgac gcacttgctc
     3301 tttgttgaat cccagcctgg tcagcagatc aaaatcgggt ttctccgcat catcaccaaa
     3361 gcccagggta ttcttaatga aatccaggcc cagagtgaag cgattcatca agaatcctag
     3421 ttcaaacgcg gatggaagtg cctcatcgat tctggccagt gcttcgtctg tgaatccctt
     3481 tgctttgaga gtttcaggat tgatttcagg cgcacctttc agtgttccgt gtcccacgca
     3541 atacttcacg atggcatctg cttgctctgc attgtatcca agtttcttga gggcaggtgg
     3601 aatgctctgg ttgatgatct tgaagtaccc accgcctgcg agtttcttga acttcacaag
     3661 tgcaaagtca ggttcaatcc ctgtggtgtc gcaatccatg accaggccaa tggttccggt
     3721 tggagcaagc acagtaactt gcgcgttgcg atacccgtgc ttttctccca gagccagagc
     3781 acgatcggat tcttcgcgcg ccgctttcag aagatagtcc gggcagtgca cgggtgaaat
     3841 tccaactgga aacacgctca atccttcgta ttcggaagca ggagcgttgt aggcagcacg
     3901 gcgatggttg cgaatcactc gaagcatgtt cttcgcattg cgctcgtatc cttcaaaagg
     3961 ccccattctt tctgagattt cagcactggt tgcatacgca ctcatgtgca gaatggcagt
     4021 caaagcgcct gcaatcgcgc gcgctttttc tgaatcataa gaatgaccca tcaccatcag
     4081 cagacttccc aggttcgcat agccaagacc cagagtgcga aatacgtgag agagtctggc
     4141 gatttcctgg gatggatact gcgccatcgt cactgaaatc tccagtacca gagtccacag
     4201 tctcgcagtg tggcgatagc caagatcgtc gaattctccc gtgtctttgt tgtaaagagt
     4261 cagcaggttt acggatgcga gattgcatgc agtgtcatcc aggaacatgt attccgaaca
     4321 tggattggat gcattgatgc ggccatcttc cgggcaggta tgccattcgt tgatggttgt
     4381 gtcatattga attccaggat ctgcgcagga ccaggcagcg taggagattt gctcccagag
     4441 atcacgcgcg cgaagagttt tggaaggctt tggttcgcga ttttgctttt gcgcgcgacg
     4501 acgctctgtt ctccagtata gattccagga agcatcgctt tccactgcgt ccatgaattc
     4561 gtttgtgact ctcacagaat tgtttgagtt ctggccggaa acggtctggt aggcacggga
     4621 ctgccagtct gtagtaagtt cttcgaatag aatggatttg tatccctggc ttgccagttc
     4681 gatcattctc tggatgtagt tttcagagat ctgcgccaga cgcgcttcat gaattgcttt
     4741 tttcagtgga gcattcttct gtctgtcgaa tttgttctcc gggttttcct ctgcgtggat
     4801 ggcggagagg atgtgattga gatgacgatt ggccagacga gatcctgcta ccatgctgag
     4861 tactttctgt tcttcgatta ctttccagct gatgaattct tcgatgtctg gatgatccat
     4921 gtccaggcag accatctttg ccgcgcggcg agtagttccg ccggacttaa tggccccggc
     4981 cgctctgtct ccgatcttga ggaaactcat gagacccgaa ctctttccac cgccggagag
     5041 agattctcct tctccgcgaa ggttagagaa gttggtccct gttccagaac cgtacttgaa
     5101 gaggcgagct tcgcgcaccc atagatccat gatgccgcct tcattcacca gatcatcgct
     5161 gatggattga atgaagcatg catgaggttg cggacgttca taggaagact gagaacgcac
     5221 caggtctcct gtcttaagtt ctacatagta atgtccctgg gactttccat ctatgccata
     5281 ggcccagtgt agacccgtat tgaaccattg tggcgaattg ggcgctgaaa tctgcgcggc
     5341 cagcatgtaa caatgttcct ggaagaagtg aaatgcatct gcttccgttt caaagtatcc
     5401 gtacttgtag ccccagtacg tccagcaacc ggccagacgt tcaaagacct gtttcccaga
     5461 agactctcca atgtatcgct tgtcttctgg aagagtggcc agtctctctt catctgcagt
     5521 acgacgctgc agccacgcag gaattccttc ttctgcgact cgctttgtat acacaggaac
     5581 gcctgctttg cgaaagtact tctgcgccag aatgtctacg gccacttgag accaggcttc
     5641 tggtacttct acggccgggg cttcaaatac aacagagccg tccgggttaa cgattttgga
     5701 tgatttcctg gtccaggtaa attgctgcca gactggattg tctgtttcgc ggtctcccgc
     5761 atgagccgca ttgaggaggg agatctggct ttgtgtgtct gctgcttttg aaaagtaacg
     5821 gttaattcgc ataaaatacc ttctgtgtat tcgaagccgg actcccaggc agcgcattct
     5881 gctttgcctc aggccgtgat tcgatttcct gtcctgaaca aatacattct aaaacggaga
     5941 tcaggcaatc agattttaag tatacacccg attaccctgg ccgctccaga attatgtccc
     6001 ctgacgggat ggcgagagtg accttcagaa agaacgggtc aataaaaaag ttcgaagatt
     6061 ggctaaaaaa atgaacatgg actcctttgc gtacaaagat agcgttttga cctggagagg
     6121 cgctgagatt cggccattgg ctgcaaaatt tggaactcct ctctatgtgt actcagaaag
     6181 cgccctggta gaacgttgcc aggccctcgg ctctgccttc ggaaacaggc gggcaggcat
     6241 ctattattcc gtaaaagcat gctcgaatat tcacattttg cgcatactcg ctggccttgg
     6301 ctgtggagcg gacattgtca gcggcggaga attgtttaga tgccgcaaag ctgacatttc
     6361 cccggagaaa atagtgtttt ctggcgtggg aaaaacagaa gcggagatcc gttatgcgat
     6421 cactgaaggg attttgtttc tcagtgtgga atcggaagca gaactgcatg aaatttctcg
     6481 catagcaacg attacaggaa agacagctcg tatcagtgtg cgcgtaaatc cggatgtgaa
     6541 ccccggaacg catccataca tttcaaccgg gttaaaagaa aataaattcg gcatcagcca
     6601 taaagaagtt gtgagaattt ataaacttgc ccaatcattg ccggggattg agccaacggg
     6661 attaggcttt catattggat cacagatcac ggatctatct gcctttgcgg atgcaggaaa
     6721 gatcgcagtg aggctcgttt ctgaaattga agctgcggga atcaaactca agcatctcga
     6781 tgtgggaggt gggctgggca ttcgttatgc aaatgaagaa gcacctgacc ctgccaccta
     6841 tgttcgaact ctcatccaat ccgtaggact cgatcacctg gaattgatgt ttgatccagg
     6901 cagaagcatc gccgggaacg ccggcatact cgtgtccaaa attctctacg tgaaagaggg
     6961 ggagaaaaca tttttcattg gggatgcggg catgaacgat ctcattcgtc cttctctcta
     7021 tgacgcctac catcacgtct tctctgatcc tttgcgcccg ggcacgaggt caggagatct
     7081 agtggggcct gtctgtgaat ctggtgactt ctttgcaaaa gatagagagc tgcccgcgtt
     7141 tgagtcgggg gacctggccg tgctcgcttc tgcgggagca tacggaatga gcatggcttc
     7201 taactacaac tccagaccca gggccgcaga ggtgctgatt gcgcgcgatg ggactgcccg
     7261 gctcattcgc agacgcgaaa cctttgagga tctgctccaa ccggaactgg atgtgtagct
     7321 cgtattttgc tcgaaaaatg gcgtggaagg ccgatatcgg aagggtgaga actgtaattc
     7381 tgtctcttct cttaactctg ggtggtcctc tttttgccgt gtcgcccgac tattcgaaca
     7441 tggggcaggt tctcgcacag aaccgcatgt accttctatt tatggataca tgcgtggtca
     7501 atcttcgcgg gccttctgcc caggaagatc caaaaggaat gcgtgcgcaa ttcgaaggct
     7561 attataaggc agccattgag cacgatttct atgcgaatct atggtatctc caggggaact
     7621 acggaaagac ctattcggaa ctcagattgt ctcaaaacgc gctccaggat ctctacagaa
     7681 gaatcctgga aaactacata gatgaaactg tcattttgct cgaagcatct gcacccatca
     7741 tcgttctgac tcgcgatcaa agcgcaaaga aattgctgga acttggtttc cgtgatctcg
     7801 aatctacgag acagatttat ctgcgcggtg caaacaccaa tccaaagatg tttcttaacc
     7861 agatcatgac ctatcaggac gggatccttc gcgctcgccg tgggcgcaga tttgccattc
     7921 tagcacttct cgaagcaaaa gttcctgtac ctgaaaaatc aaagtatcag gtggtgactc
     7981 tcgacgatgt gaagaacatg atggaagagg gagagaagca gacgaagtat gtagagatcc
     8041 tgaaccttct tgtgaacatg actggaagaa acctggtgcc caaggtcatt gcaagcgacg
     8101 tgcttggaaa gatgatcagc ctgaatactt tagaagtgca tcaggacaac tacggcagaa
     8161 tgtactcaga cagacgaagt gtgtttcagt tgatcactgt aacactgaga tcagatgaat
     8221 ttcataagtc tgattccatg cccaggagaa acacggacaa tcgcaatgaa gtgcctgctt
     8281 ctgattcaga tccaatcaag ccggaaggaa ccacaacgca gccacagcct caagcaatca
     8341 ctcctgtgac tccagcgccc gctaccgatc cggggaagaa gtagcgctca gcgctcttct
     8401 tcctggcgac caatcaaaaa gctgaggctt gcttctacta cgctcgaaac tggcttggtc
     8461 atattctcta tgatctgacc gcccatcgca gagttgagag cttctttaaa tccttctctc
     8521 actcccgcgc gaaacttggg catgaggtcg agagcttctt ctctcaagcc ttctttcact
     8581 tttgattttg caagctccag tttttcgtcg agctttctgt cgagcagatc acgaaggtgg
     8641 agaatgtaga atgcgtagga tagtccaaga gtgaacaatg aggaaacgac ggccgtaatt
     8701 actattgtgc ccaggagttg tatcatggtt tacctgctcg aaatgagtct aacgcggata
     8761 taaggtcggt caattcattt tctggatccg gctttctaga agggtccccc ctggtccata
     8821 cgagagagtc ttccggcagc tctgataaaa aacgggacgg ttccatatct atgatttcgc
     8881 cgaacttttt ctttgttctc gatgcggtca aatacagaac gtccatagcg cgagtcattc
     8941 caacgtagaa gagccttcgc tcttcagcca ctgtgatttc aatgtctccc gattcgctga
     9001 gggatctttc cggagggaat aaaccgtcca tgagaccgga tagaaatacc accggaaact
     9061 caaggccctt ggatacgtgc actgtcatga tttgcgcgcg cccacctgat tcttcttcat
     9121 cgtctgaagt gagaagcgaa actgctttga gaaaatcaaa gatagtgaag tcttctcctt
     9181 cctgccgcgc ttccgtttcg agagattgca tcattccaag taattctgaa atattctgca
     9241 ttcgcatcga aacggcagaa tcttcttccc cttcgcggcg aaattgagaa tcaaacttga
     9301 gttcgtacac aagggcccgt gctgcctgag tgactcctct ggcaaaagcg gggcgatggc
     9361 gctccatgat ttccatgagc tccgcgatga atgcagatcc ttctcttttc aattgcacga
     9421 ggcccggctc gccctgcatg cgaactagaa attcgtagag gcctgtgtct ctcgacgctt
     9481 ctcgaattcg cgcgatggat acttctccaa ggccgtttcc aggtctggat aaaatgcgga
     9541 tcagacttag atcgtctctg gggtttgcga tgattcgcag gtaggaaagt acatcgcgca
     9601 cctcacgcct gtcaaaaaac cgatatcctc ccacgactct gtgcggaatt ccagcacttc
     9661 taaatgcatt ttcaaacgca cgagactgga aattcgttct gacaagaact gcaaaatctt
     9721 ccggtttgcg gcgctctctt acaattctct gcctgatctc ttctgcaacc agggccgctt
     9781 cttcttcctc atctgttccg tatattccaa gaatcttttc tccaggcgga cgaacactcc
     9841 tgatttcctt tggcgccctg gacgtgtttc gaacaatcac ggcactcgca gcacgcacaa
     9901 tttccgcctg agatctgtag ttcttttcca gccgatgtac acttgcaccg gcaaaatctt
     9961 tgtgaaaatt taagatcagg ctgatgtcag atccccgaaa tgcatagatg gactgatcat
    10021 cgtctcccac tgcgaaaata ttgcgatgag gcagcaccag aaggcgcaga aactcatatt
    10081 gcattgggtt tgtatcctgg aactcatcca ccagatagtg agtgcgctcc tttctgtact
    10141 tctcaaggag ctcctgatcc ttgtgaaaca ggcgaatggg aaggaggatc aaatcgtcaa
    10201 agtcaaggct gttggtgctg cgaagagcgt gctcgtactc ctcatagaaa tcaatcactc
    10261 cttcatccag gccattggat tcgaagaaac gcgcgggatt ggccagagcg tttttcgctc
    10321 gcgaaaacca ggagaggagt ccgtctttct ttgcgtctga cggatcgagc ttgtggcggg
    10381 aatagatttc agaaagcacg cgaagcctgt cgtcttctga taagatggta aatggattcc
    10441 cgtatccaag cttttcaatg tgagaacgaa ggattctcgc tccaagtgaa tgaaatgtgc
    10501 ttacgatcaa gcctcggcgc ttttcgcggg gcagtttgga gagtctttct ttcatctccc
    10561 gcgcactttt gtttgtgaac gtcacagcag tgatgtgcgc ggccgcgaca cctgattcga
    10621 tcatgcgctg gattcgctga gtgaggacgc gtgttttgcc ggaacccgca cccgcaaaca
    10681 caagtactgg tccgtgcac
//
LOCUS       DAORFF010000041        10541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2878_length_10541_cov_4.083349, whole genome shotgun sequence.
ACCESSION   DAORFF010000041 DAORFF010000000
VERSION     DAORFF010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10541
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2878_length_10541_cov_4.083349"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1807
                     /locus_tag="PKK76_02965"
     CDS             <1..1807
                     /locus_tag="PKK76_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNL67454.1"
                     /translation="KYIPYPVVAGYMSSVGIIIVIGQIPRILGSTPLAEFEHGVSPVH
                     LAAAIIATATIAAMFIVPRITSVMPAPIFGLGAGLVAYGLFALAFPEYRSLEGNVLVV
                     GPLHGEFSFDVFFSRFRSYATLQGDYLREAVLAGGTLAVILSIDTLKSCVVVDTVTRS
                     RSDSNRELLGQGLGNITSAFCGGLAGAGTLGATLVNVSSGGVTRISGVLAGLFALIAL
                     VFLSPVFAWVPIPALSGILIVVGLRMIDWKLAALLRRKATALDFFVILTVIGVALKFS
                     LMAAAGAGVALSALFFMREQMRGSVVHKKTYGSEISSKRSRLPAEQKVIRASGQRAVL
                     YELQGSLFFGNTDRLFTLIHADLEESQFVIISMRRVQSIDSTAIHMLLQINTLLEDRG
                     GRLFITDLPESLEEGQAPEEFLHDAGLLGPASGIVLFDEFYYALEWVEDYILKENDLY
                     HSHLERPLELGEIEVFENVDAEDLPLIQQCLESRSFSAGNTIFSSGDTSDNLFFIRRG
                     VVRIMLPINSHRQHHLASFGRGDFFGDMAFVDHEVRSAHAIAETDCDLYSLSRNVFDS
                     LLAKDPQIGRQFYENLVRVLAHRFRAVHLELRSLE"
     gene            2219..3109
                     /locus_tag="PKK76_02970"
     CDS             2219..3109
                     /locus_tag="PKK76_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL67455.1"
                     /translation="MFSSRALKWIVGLPAAIATLLVAVAWYFSSLVLYPPVHCNPDHH
                     VYCGNPSEIGLAFEDVQIPTSDGLKMQSWYIPAERSTKAIVMVHGHGGLKNEGLRFSR
                     ALHDAGYNLLLLDLRRNSGGSATMGYLESRDVIRALDFLSTEKKNEKLGIFGFSMGAA
                     TSILSMAADQRVRAGLFSSGYASAVDVLSEAAARDYHLPYYPLFPVMLFVGDHRAHIE
                     LEAVRPVDVIGGISPRPISIFHCDKDDYVESSHAQRLFAAAREPKEIWTPPCNRHERI
                     WNVHREEAERRAVAFFKKNL"
     gene            complement(3113..4021)
                     /locus_tag="PKK76_02975"
     CDS             complement(3113..4021)
                     /locus_tag="PKK76_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNL67456.1"
                     /translation="MHTPTINSMRKISILLFLAATSCAHQGFVFRSVHGFSPATNAAL
                     EKFLEENKSHKGRKVAVFDGDGTVLGQVPHYLADECLYRYAKAHPDRRPEIIARMKHK
                     SNVSIPYVQDRVFYLAGMTLDEVRQMGTQCYESDYRGKIYTPMKDLIELLKDNDFEIW
                     IITASPEGLYQEFLSHELQISPTHIVGVKSVIRGGIITDRIVEPIPQDAGKKEAVETF
                     VQTTPLLAAGNSRGDREMIESSSGIRMIINPDEHLESGEKISIAQYAKDHNWLVEKIN
                     DVEESGFPAVSSKSYGIRKNRAHPVP"
     gene            4116..4694
                     /locus_tag="PKK76_02980"
     CDS             4116..4694
                     /locus_tag="PKK76_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNL67457.1"
                     /translation="MKEKQGPRERIVSAAGSLFYRRGFGTGINEILGESGAHKLSLYQ
                     YFPSKDDLGREYVERQKEQILGFLAKLIQERPDPRDFIKSWVQVIKRQARKKEFFGCP
                     FANFAAQTLEKKPEFRDQLGGVLLEWQQMFSDYFAKWQASQSRSGRVSPAQLARRTLA
                     IYEGNVQLYLISGDAGYLDAIEADFVEMIRST"
     gene            4788..7226
                     /locus_tag="PKK76_02985"
     CDS             4788..7226
                     /locus_tag="PKK76_02985"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNL67458.1"
                     /translation="MEPVSQSHWEDLGNSIKRHIVFVLGNDFQPPRKDTYYSGLAYCV
                     RDRLIERWLSGQRLAYETHPKRVYYMSMEFLPGRFLKNFIINLGMEAESREALKESGF
                     ALEELEEQEPDPGLGNGGLGRLASCYLDSMACLNIAAHGYGIRYDYGIFHQKIVNGWQ
                     VEQCDNWLQFGNPWEITRRHFLYTVKLFGRSEPYHDERGNLRYRWVDTDTVDAMACDI
                     PIPGYNNETVNNMRLWAAVSSRDFNLDFFNHGDYVRAMEEKVLSENISKVLYPSDEKE
                     QGRELRLKQQYFFVAATFEDLIRRFKKDHKSFQALPDRVAVQLNDTHPTIGVAELMRI
                     LVDEEGIPWDEAWAISTKVFAYTNHTVLPEALETWPVGLLGRVLPRHLEIIFEINHRF
                     LDWVRKERPGDEGLLSRVSIIEEGPVKKVRMANLAIVGSHSVNGVAALHSEILKKSLF
                     RDFHELYPGKFKNITNGITQRRWLLQANPLLSDLITKTIGPDWIVDLYKLEKLLPHAE
                     DKAFRTEWMKVKRANKERFSTYVYEQTGVRPDPDAMFDMQTKRIHEYKRQVLKVLHVI
                     TLYNRIREGKTIAPRAVFIGGKSAPAYYMAKLTIKLVNSVANVINHDPAMHGKLHLVF
                     LPNYCVSQAEKVMPAADLSEQISTAGMEASGTGNMKFSLNGALTIGTLDGANIEIMEE
                     VGPENFFVFGLKSEEVDHMRATGYRSRDYYERDPELKAALDMIGSGAFSPEQRDLFLP
                     IVQGLLDRGDRFFVLADYRSYITAQESVGMLYRDLDAWGRKSVLNVAKMGKFSSDRSV
                     REYAEKIWNIPTTR"
     gene            7244..7543
                     /locus_tag="PKK76_02990"
     CDS             7244..7543
                     /locus_tag="PKK76_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNL67459.1"
                     /translation="MDKIVVQVPSDLSELIPGYLKNLSKYADQLKELISAGDLAAAGR
                     IGHNLKGSGGGYGLDEISTIGKLIEEKAHASDASAVKEALQKLNDYLSRLHVEFS"
     gene            7666..>10541
                     /locus_tag="PKK76_02995"
     CDS             7666..>10541
                     /locus_tag="PKK76_02995"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNL67460.1"
                     /translation="MTGYFAVTFLNPAFFRSGGTPGFTIRQSTPCITSEGGLNHTFTV
                     ETNTTPGALVTVPVSSLLTTEGVVSPSSVSLSVAGGTQTITVSGVDDGSVDGNQSYTI
                     ALGNASSYDPSYNNLSVGSVSCVNLDDEQRRVSAWPLTGLTVAESGTTATFSVLLSSA
                     PASNVIVDVTSLDTTEGTVSPASLTFTPANWSTAQTVTVTGVNDFLQDGSIAYNVALG
                     INAASNAAWVATGTTDTVAVTTTDDDTAGVTVTPTSLSTVESGVAKTFSVVLTSQPTS
                     SVTISAASADTTEGTVAPPSRTFTAGNWNVPQSFTVTPQADAILDGTITYNVTLGPVV
                     SADANYSGMIVSPVSVANADAGIPDIVVSPTSGLVTGEGGVNATFTVVLTQQPSSNVV
                     VGISSSDTTEGTVAPATLTFTNANFATPQTVTVFGVNDAILDGNIAYTIVTAPAVSAD
                     PNYSGLNPPDVSVTNNDNDQNLVNISSSTNNLYTTEGGGQVILKVVLSAQPLQDVDVG
                     PITVDDGTEASVSPGTLTFTSANWNVAQDVTVTGLPDAVVDGTIIYHVDLGTTTSTDL
                     NYNGITPTDYNGGAAGQVTIRNADINGATKIVRCDSNSLTTTEAGGTALMFYTLTQAP
                     SANVDIPFSSTDTTEFTVSPATLTFTPANWNTCRQVTLTGVDDAVDDGNQTRTLTIGA
                     ATSGDAAFNGIDLADPNVTNNDNDTAGYTVSAISGNTTEAGGTATFTIRLNSKPTNNV
                     TVNLSSSNVAEGTVSPASVTFDATVGACMGGGDWCTNQTVTVTGVNDTAKDGNIGYSI
                     ITAQSTADPVYSLINPADVAVTNVDNEKYIFITASTYDGAFDFNASLAGGANSAVNGD
                     GNPRQEMDNACQTDANKPASGTFRALAVDASHRRASLTANLGDSQISWVLSPLYDYIR
                     YPGGATLFTSDANSIFVFGSLTNNFGAGSTYWTGLNGDWTTGSH"
BASE COUNT         2552 a         2529 c         2764 g         2696 t
ORIGIN      
        1 aaaatacata ccatatccag tcgttgcggg ttacatgagc agtgtgggga tcatcattgt
       61 aattggccag ataccgcgaa ttctcgggtc cactccactt gctgagttcg agcacggtgt
      121 ttcgccggtt catctggccg cagcaatcat tgctacagcc accattgcgg ctatgttcat
      181 tgttcctcgc ataacatctg ttatgccggc gccgatcttc ggtcttggcg ccggtctggt
      241 ggcctacggt ctgttcgctc tggcttttcc ggagtatcga tcattggagg gaaacgtgct
      301 ggtggttgga ccactgcacg gagagttctc tttcgatgtc ttcttttcca ggttcagaag
      361 ttacgcgaca ttgcagggag attatttgcg ggaggcggtt cttgccggtg gaactctggc
      421 agtcattctg tccatcgata ctctcaagtc ctgcgttgtg gtggatacgg tgaccagatc
      481 tcgcagcgat tctaatagag aactgctcgg gcaaggtctg ggaaatatca cttctgcgtt
      541 ctgcggtggc ctggccggtg cgggaactct gggagctaca ctggtcaatg tttccagcgg
      601 cggtgtcaca cgaatctctg gcgttcttgc cggactcttt gcgctcatcg ctcttgtatt
      661 tctttctcca gtttttgcat gggttccgat tcccgctctg tctggaattc tgatcgtagt
      721 tggtttgcga atgattgact ggaagctggc ggcgcttctt cgcagaaaag caactgccct
      781 ggatttcttc gtgatcctga ccgtcatcgg cgtggcactg aagttcagct tgatggcggc
      841 ggcaggtgcg ggcgtcgcgt tatccgcgct ctttttcatg agagaacaaa tgcgcggatc
      901 ggtcgtgcat aagaaaacat acggaagcga aatatcttcg aagagaagca gacttcccgc
      961 agaacagaaa gtgattcggg ccagcgggca gcgtgcggtg ctgtatgaat tgcagggcag
     1021 tctgtttttt ggaaatacag atcgattgtt cactctgatt catgcagatc ttgaagaatc
     1081 gcaattcgta atcatcagta tgcgtcgagt tcagtccatc gattctaccg ccattcacat
     1141 gcttttgcaa atcaacactc tactggaaga tcgcggtgga cgacttttca tcacggatct
     1201 gccggaatct ctagaagagg gacaggcgcc ggaagaattt ttgcacgatg ccgggcttct
     1261 cgggcctgct tcaggcatcg tattgtttga tgaattttac tacgcactgg aatgggtgga
     1321 agactacatt ttgaaagaga acgatttgta tcattctcat cttgagcggc cccttgaatt
     1381 aggcgaaatc gaagtctttg agaatgtgga tgcggaagat ttgcccttga ttcaacaatg
     1441 tctggaatct agatcattta gcgcgggcaa tacgatcttc tcatccggag acacaagcga
     1501 caatttgttt ttcattcgac gcggtgtagt gcgaatcatg ctgcccatca acagtcacag
     1561 gcaacatcac ctggcctctt ttggtcgcgg agactttttc ggagatatgg cttttgtgga
     1621 tcatgaagtc cgttcagcgc atgcaatcgc tgaaacggat tgcgatttgt acagtctttc
     1681 ccgtaacgta tttgattctt tgcttgcaaa agatccacag attggaagac agttctacga
     1741 aaaccttgtg cgcgttctgg ctcatcggtt cagagcggtg catctggagc ttcgatcgct
     1801 ggagtgaata tgagtcgact ttgcatccga agtctctttg cgccaaccct ggcgctgggc
     1861 ctgatcgccg cctgtgcggg cagttcgtct caggcagggg gaaggcacag gtccctcggg
     1921 aagcccatcg tcttgactgg cattgccggg aacgccaaaa tttccgccca tgtggagtcg
     1981 ccggaattga gtgtctactg tctcaatcgt ccctcgtgga aaaagacgtt ggatggaaag
     2041 cgcataacgg tccgaggaac cctcgagtac acggatgcgt tcgccgcgac aaaaaaaaat
     2101 ggtgaaatca gtcagggtac agacggcggg gtttacgtga tcaggaattg cgaactggta
     2161 gatcctcccg aggaataaaa tcggtttgca aatatttcaa tcgcaatgac tttcggcgat
     2221 gttttcatca cgcgctttga aatggatcgt gggtctgccg gcggccatcg ccacacttct
     2281 cgtcgccgtt gcctggtatt tttcgtctct ggtgttgtac cctccggtgc attgcaatcc
     2341 ggaccatcac gtttattgtg gcaatccttc tgaaattggc ctcgcctttg aggacgtgca
     2401 gattcctacc tcagacgggt tgaagatgca gagctggtat atcccggcag agcgttctac
     2461 aaaagccatc gtgatggtgc atggccacgg tggactgaag aacgaaggtc ttcgcttttc
     2521 gcgcgcgctc catgatgcgg gctacaattt actcttgctt gatctgcgaa ggaatagcgg
     2581 tggatcggcc accatgggat atcttgaatc cagagacgtg attcgagccc tcgattttct
     2641 ttcgacagag aagaagaacg aaaagctggg tatctttggt ttttccatgg gtgctgccac
     2701 gagcatccta tccatggcag cagatcagag agttcgcgcc gggctcttta gcagcggtta
     2761 tgcgagcgca gtagatgttc tgtcagaggc ggcagcccgt gactatcacc ttccgtacta
     2821 tcctctgttt cctgtgatgt tgtttgtagg agatcatcgc gctcacattg aactcgaagc
     2881 ggtgcgtccc gtggatgtga tcggaggcat cagtccgcgt ccaatttcca tctttcattg
     2941 cgacaaagac gactatgtcg aatcgtctca tgcgcagagg ctttttgccg ctgccaggga
     3001 acccaaagag atctggactc caccatgcaa tcgacatgaa cgcatctgga acgttcaccg
     3061 cgaagaggcg gaacgccgcg cggttgcttt cttcaagaag aatctttaga tgctatggaa
     3121 cagggtgggc tctgtttttt ctgatgccgt aactcttcga agatacggca ggaaacccgc
     3181 tttcttctac atcattgatc ttttcaacta gccagttgtg atccttcgcg tactgtgcga
     3241 tgctgatttt ttcgccggac tcaagatgct cgtctggatt gatgatcata cggattccgc
     3301 tgctcgactc gatcatttcg cgatcccctc gactgttacc ggcggccagg agaggggtgg
     3361 tctggacaaa agtttctaca gcttcttttt tccccgcatc ttgcggaatg ggttccacga
     3421 ttcgatctgt aatgattccg cctcgaatga cagacttcac accaacgatg tgagttggcg
     3481 aaatctgcag ttcgtgactt aagaattcct gatacaatcc ttctggactt gctgtgataa
     3541 tccagatttc aaagtcgtta tcctttaata gttcaatcag atctttcatg ggagtatata
     3601 ttttgccgcg ataatcactc tcgtagcatt gcgtgcccat ttgtcttact tcgtccagag
     3661 tcatccctgc cagataaaaa acgcggtcct gcacatacgg aatagaaacg ttcgatttgt
     3721 gtttcatgcg tgcgatgatt tcaggtcttc tatctggatg cgctttcgca tagcgataca
     3781 gacattcgtc cgccaggtaa tgagggacct gtccgagcac cgttccatct ccgtcaaata
     3841 cggccacctt acgaccctta tgcgatttgt tctcttccag aaatttttca agcgctgcat
     3901 ttgttgcggg actgaacccg tggacgctgc gaaacacaaa accctggtgt gcacaggaag
     3961 ttgcggccag gaacagaaga atagaaatct tgcgcattga gttgatggtg ggagtgtgca
     4021 ttcgagcgcg tcgagcaaaa atgcatcgcg gtttttggcg attcgagccg aattggacta
     4081 gacagatcgg tccagccttc tttttgtcgg aggctatgaa ggagaagcaa ggcccccggg
     4141 aacgcatcgt cagcgcggcc ggttcccttt tttacaggcg cgggtttgga acgggtatca
     4201 acgagattct gggagaatct ggcgcacaca agctcagcct gtatcagtac tttccgtcca
     4261 aggatgacct gggtcgtgag tatgtagaaa ggcagaaaga gcagatactc ggttttcttg
     4321 cgaagctcat ccaggaacgt ccggacccac gtgatttcat caagagctgg gtgcaggtga
     4381 tcaaaaggca ggccaggaaa aaggagttct ttggttgtcc attcgccaat tttgcggctc
     4441 agactctgga aaagaaacct gaatttcgag atcaactcgg cggggtactg ttggaatggc
     4501 agcaaatgtt ttctgattat ttcgcgaagt ggcaggcctc tcaatctcgc tctggtcgag
     4561 tttcgcccgc tcaacttgcc aggcggactc ttgctattta tgaaggcaat gtgcagctct
     4621 acttgatttc aggagacgct ggttatctgg atgcaatcga agctgatttc gttgaaatga
     4681 taaggagcac ctgaagtaac tttcgacaga agaaccatga catttttcgt ctgacgcctc
     4741 ctggttttac ggttgcttcc ttttgcaaac ctggcgcatc atgagttatg gaacctgtat
     4801 cgcaatcgca ctgggaagat ctaggcaata gcatcaaacg tcatattgtt tttgtacttg
     4861 gaaatgactt tcagcctcct cgcaaagaca cctactacag tggtcttgcc tactgcgtgc
     4921 gtgatagact cattgagcgc tggctttctg gacaaagact cgcttacgaa acacatccca
     4981 agcgcgttta ctatatgtcc atggagtttc ttccaggcag attcttgaaa aactttatta
     5041 tcaaccttgg catggaggct gaatcgcgcg aagcgctgaa ggaatcaggt ttcgcacttg
     5101 aagaactgga agagcaggag ccggatcctg gtctgggcaa cggtggtctg ggtcgtctcg
     5161 catcctgcta tctggattcc atggcctgct tgaacattgc agcgcatgga tatggaattc
     5221 gatacgacta cgggattttt catcagaaga ttgtgaacgg ctggcaggtg gagcaatgcg
     5281 acaactggct tcagtttgga aatccgtggg aaatcacgcg cagacacttt ctgtatactg
     5341 tgaaactatt tggcaggtca gaaccatatc atgatgaaag aggaaatctt cgctatcgct
     5401 gggtggacac cgataccgtg gatgcgatgg cctgcgacat tcccattcca ggatacaaca
     5461 acgaaactgt aaacaacatg cgcttgtggg cggccgtatc cagtcgtgat ttcaatctgg
     5521 atttctttaa tcacggggac tacgttcgcg cgatggaaga gaaagttctt tcggagaaca
     5581 tctcgaaggt gctgtatcca agcgatgaaa aggaacaggg ccgagagctt cgcctcaagc
     5641 agcagtactt ctttgtggcg gctacttttg aagatttaat tagaagattc aagaaggacc
     5701 acaagtcttt ccaggctctc ccggatcgag ttgccgttca attgaatgac actcatccca
     5761 ccattggcgt tgcagagttg atgcgcatac tggtggatga agagggaatt ccctgggatg
     5821 aagcctgggc catcagtacg aaagttttcg catacaccaa tcacactgtt ctcccggaag
     5881 cacttgagac ctggccggtt ggcttgcttg ggcgagtgct cccaaggcat cttgaaatca
     5941 ttttcgagat caatcacaga tttctcgact gggttcgcaa agaaagaccg ggggatgaag
     6001 ggctgctttc gcgcgtgtct atcattgaag aaggtcctgt aaagaaagtg cggatggcca
     6061 atcttgcaat tgttggaagt cactcagtaa atggagtcgc ggcacttcat tctgaaatct
     6121 tgaaaaagag tctgtttcga gactttcatg agctgtatcc gggaaagttt aagaatatta
     6181 caaatggaat tacgcagcgc cgctggctgc ttcaagcaaa tccacttctg tcagacctga
     6241 tcacaaaaac cattgggccg gattggatcg tggacctgta caaactggaa aaacttctcc
     6301 cccacgcgga agacaaagcg tttcgcactg agtggatgaa ggtcaaaaga gcaaacaagg
     6361 aaagatttag cacgtatgtg tacgagcaga caggagtgcg tccagatccg gatgccatgt
     6421 tcgatatgca gacaaaaaga atccacgaat acaaacggca ggttctgaaa gtgctgcacg
     6481 tcatcactct ttacaatagg attcgcgaag gaaaaacaat cgcaccgcgc gctgtgttca
     6541 ttggtggaaa atctgcacct gcctattaca tggccaagct gacgatcaag ctcgtgaatt
     6601 cagtcgcaaa cgtcatcaat catgatcccg cgatgcacgg taaacttcac ctcgtgtttc
     6661 ttccgaacta ttgtgtttcg caggcggaga aagtaatgcc ggcggcggac ctatcggaac
     6721 agatttctac agccgggatg gaagcatccg gaacagggaa catgaaattt tcgctgaacg
     6781 gtgctctgac cattggaaca ctggacggcg cgaatattga aatcatggaa gaagtgggac
     6841 cggaaaactt ttttgtgttt ggactcaaat cagaggaagt ggatcacatg cgtgcgacgg
     6901 gctatcgatc acgtgactac tacgaaagag atcctgaact caaagcggca ctggatatga
     6961 ttggttcagg cgcattttcg cctgagcaaa gagatctatt cttgcctatc gttcagggcc
     7021 tgttggatcg cggggatcgt ttcttcgtgc tggcagacta tcgttcttac atcacagctc
     7081 aggagagtgt ggggatgctg tatcgcgatc tggatgcctg gggtcgcaag tcggttctca
     7141 acgtcgcaaa aatgggaaaa ttctccagtg accgatccgt gagagagtat gcggaaaaaa
     7201 tctggaatat tccgaccact cgttgaaatg agagagggaa gccatggata agatcgtagt
     7261 tcaagtgcct tcagatcttt ctgagctgat acccgggtat ctcaagaatc tcagcaaata
     7321 tgctgaccag ctcaaagagc taatctcggc gggggatttg gcggcggctg gacgaatcgg
     7381 tcacaatctg aagggttccg gtggcggata tggtctggat gagatttcta ccattggcaa
     7441 attgattgaa gagaaagcac atgcaagcga tgcttctgcg gtaaaggaag ctttgcagaa
     7501 gctgaatgat tatctgtctc gactgcatgt agaattctcc tgaaaaatgg cttgtcaagc
     7561 cccaggttcc atgccgatct ttcaattagc accatgggaa agtttgcaaa gagctgcggt
     7621 ctggtttgct tatgtctctt ttcttcttgc ggccagtatg atgccgtgac cggctacttt
     7681 gctgttactt tcctgaaccc tgctttcttt cgcagcggcg gaactccagg gtttacgatt
     7741 cgccaatcca cgccttgtat cacatccgaa ggaggattga atcacacttt caccgtggag
     7801 acaaatacaa ctcccggtgc cctggtcacc gttcctgtaa gttctcttct gaccacggaa
     7861 ggtgtggttt cgccgagcag cgtgagtcta tctgttgccg gcggaacgca aacgattacc
     7921 gtttctggtg tggatgatgg atcggtagat ggaaaccaga gctacacaat tgcgctgggc
     7981 aatgcatcga gttatgatcc ttcctataac aatttgtctg tcggttctgt gagctgcgtc
     8041 aatctcgacg acgagcaaag aagagtgtcc gcatggcctt tgactggatt gactgtagct
     8101 gaaagcggaa ccactgccac tttttccgtt ctactcagtt ccgctcctgc ctctaacgtg
     8161 attgtggacg ttacttcgct tgatactaca gaaggaacag tcagtcccgc gagtcttaca
     8221 ttcactcccg caaactggtc caccgcgcaa acggtgactg tcacaggtgt gaacgatttc
     8281 ttacaagatg gatcaatagc gtataacgtt gctcttggca tcaatgcagc gtctaacgcg
     8341 gcctgggtgg ctaccgggac cacggacact gtggccgtta ccactacgga tgatgacact
     8401 gcaggggtga ctgtgactcc aacaagtttg tctaccgtcg aaagcggtgt cgcaaaaact
     8461 ttttctgtcg tgctcacttc gcagccaact tccagtgtta cgatttctgc tgccagtgcg
     8521 gatactacag aaggaaccgt tgcacctccg agtcggacat tcactgccgg aaattggaac
     8581 gtgccgcagt cctttacagt cacgccgcaa gcagatgcga ttctggatgg aaccatcact
     8641 tacaatgtaa ctctgggacc agtggtgagt gccgatgcga attacagcgg catgatcgtg
     8701 tctccggtga gtgtagccaa tgcggacgca gggattccgg acattgttgt cagtccgacc
     8761 agcggtctgg tcactggaga aggtggagtc aacgctacct tcacggtcgt tctcactcag
     8821 cagccatcgt ctaacgttgt agtagggatt tccagttcag acacaacaga aggaacagtt
     8881 gctcccgcga ctttgacttt caccaacgcg aatttcgcaa ctccgcagac tgtcacagta
     8941 ttcggcgtca acgacgcgat cctggacggg aacattgctt atacgattgt gacggccccc
     9001 gcagtcagtg cagatccaaa ttacagtgga ctcaaccctc cggatgtgag cgtcacaaac
     9061 aatgacaacg atcaaaacct ggtgaatatt tcttccagta caaacaatct gtatacgaca
     9121 gagggcggcg gtcaggtaat cttgaaagta gttctatccg ctcagccact ccaggatgtg
     9181 gacgttggcc cgatcacagt ggatgatgga acggaagctt ctgtttctcc aggtacctta
     9241 actttcacca gcgccaactg gaatgttgct caggatgtaa cagtgaccgg cctcccggat
     9301 gcagtggtgg atggcaccat catctaccat gtggatctgg gaaccacaac gagcactgat
     9361 ttgaactaca atggaattac gcctacggat tataacggcg gggctgcggg acaggtcacc
     9421 attcgcaatg cggacataaa cggtgctaca aaaatcgtgc gatgtgattc caactccctc
     9481 acaacaacgg aagcgggcgg aacggcgctg atgttctaca ctctcacgca ggcgccttcg
     9541 gcgaatgtgg atattccttt ctcaagcaca gatacaacag aattcacagt ttctccagct
     9601 acacttactt tcacgcctgc aaactggaac acttgcagac aggtcactct cacaggcgta
     9661 gacgatgctg tggatgatgg caaccagacg cgaactttga cgattggtgc agccacgagt
     9721 ggggacgctg ctttcaatgg aattgatctg gcggatccga atgttacgaa caatgacaat
     9781 gacactgcgg gctacacagt atctgcgatc agcggaaaca caacagaagc gggtggcacg
     9841 gctactttca cgatacgttt gaattctaaa cctacaaaca atgttacagt caacctttcc
     9901 agttcgaatg tcgccgaagg cacagtgtct ccagcaagcg tcacattcga tgcaaccgtg
     9961 ggagcgtgca tgggcggagg cgattggtgc acgaatcaga ctgtgactgt gaccggcgta
    10021 aacgataccg cgaaggacgg aaacatcggt tactctatca tcacagctca gtccacagcg
    10081 gatcctgtgt actcattgat caatccagca gatgttgcag ttacaaatgt ggacaatgag
    10141 aagtacatct tcatcaccgc cagtacgtat gatggtgcat ttgatttcaa tgcatctctt
    10201 gccggcgggg cgaattcggc agtgaacgga gacggcaatc caagacagga aatggacaat
    10261 gcatgtcaga ctgacgcgaa caaaccagcg agtggaacat tcagagctct cgcagtagac
    10321 gcaagtcaca ggcgtgcgtc tctgactgca aatctaggag actcccaaat atcctgggtg
    10381 ctttctcctc tttacgacta cattcgatat ccaggtgggg cgacattgtt cacatcagat
    10441 gcgaattcca tttttgtttt tggtagtctt acaaacaatt ttggagcagg aagcacttac
    10501 tggacaggcc tcaatggaga ctggaccact ggatcgcact g
//
LOCUS       DAORFF010000042        10437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2908_length_10437_cov_3.857831, whole genome shotgun sequence.
ACCESSION   DAORFF010000042 DAORFF010000000
VERSION     DAORFF010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2908_length_10437_cov_3.857831"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PKK76_03000"
     CDS             complement(<1..609)
                     /locus_tag="PKK76_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNL67461.1"
                     /translation="MIHKREFHDLEKKFDLVVIGGGITGISVAREAAVRGIRVLCVEK
                     NDFACATSSATSKLLHGGLRYLESYEFGLVRESLSERHIMGLAAGHLSRPMQFLVPMF
                     EWSNPGRFIMQMGLSTYDLLAYDRNLGTPEDKQIPPAKWISREEFLKLEPKIESKGLK
                     GGFVYYDYQSTYPERLALAFVKTAVQAGAVMLNHCQATGFGFK"
     gene            complement(606..1781)
                     /locus_tag="PKK76_03005"
     CDS             complement(606..1781)
                     /locus_tag="PKK76_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNL67462.1"
                     /translation="MAKFPLNSFPELAPSHPADISKSIQKEYGRTLDDFVDGDVFLHP
                     RAFTIDRTFAQEFATVFHESSPLFLSAPYAQAHGFADMLVHPLQVFNVILSLGVQNNS
                     EKAIANLGYYNVQFLKPVYPGDTLTGRTRVLSKKVRGEGKPGIVHVNTLGLNQRGEVV
                     IQYERKIMIPPGDGKSAPKRGDANLPFPVVKDPSCELPDAKSLSTDMKHHTLSNTYFE
                     DFEAGQILVHKNMRTITDEHVPWTYRMGNTHPLHYDRLYSAGLSGPMSGEPIVYGGLV
                     FGWLCGLASRDLTENMLWDLGYTEGYHTAPAISGDTVGSISRIVAKEEGPMPNTGILH
                     VQLIGVKNVKTKEAFDKHGADLFIKENDKKDMGKEKIPEKIFEIERRILLKKKGYGA"
     gene            complement(1827..2687)
                     /locus_tag="PKK76_03010"
     CDS             complement(1827..2687)
                     /locus_tag="PKK76_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67463.1"
                     /translation="MVSTIGSSVLLLKSEVQNLLQPTSHFLTCGCSVLKYEGHVSLVL
                     LIDADPNQVSKFEHAMQGSEVRLLTASNLARGLELAKSGSPDLIMVGATEKSTDSISV
                     ISNLRKEHTTREIGICLISAPLEKGLILRLKQLAVLDIVPPSMQAADLRGRVERAVAV
                     AEKLRLQQELKRANHINVSRKGGRTQVTVLSNLKDFALPEAKVVFNHFFLKLIKPDIV
                     ILDIRQVPEIPPTELRILDQFVTVLGGEKVLVLAGKHLGSIVSQTEIGSRNKVFFSSE
                     EMDTFLARKK"
     gene            2707..3849
                     /locus_tag="PKK76_03015"
     CDS             2707..3849
                     /locus_tag="PKK76_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL67464.1"
                     /translation="MALLCAYYFYSVSHKPVLHFQENEFHDQILREVPVLTRAYHPTP
                     WCFNNHLMLLLLLFREYRSKDYPFDRIDQLRMKDGGITGLAWSGWNEQNALLKNKKKS
                     IMPIVVIFHTISGDEQDVKGIVEYVRVSLRWTAVVCIRRGHGNLPLGKPKINTMGSTS
                     DLSEQLAFIQKKFPGAPLFGIGISAGSGLLARYLGESGKKSKFMAAAAISPAYDIEKA
                     FHRIHPVYSKIMGQRLISYFLKRHYDSLSRVHGFDHAMQAQTIGEFQDRIHAIAGYKT
                     KEEYYRFSNPIKVAKNIRTPLLILNSQDDPICVNLNVLENLHWLENLPHTMLVHTKRG
                     SHVAYFEGWKAKPWCDRVVGDYFQAVLKTEAPTAKRRPSKKKQVRR"
     gene            complement(3866..4342)
                     /locus_tag="PKK76_03020"
     CDS             complement(3866..4342)
                     /locus_tag="PKK76_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002763805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNL67465.1"
                     /translation="MKNVQQWFDEYAESHRNETNQTIHWICIPLIMFSTLGLFWAASD
                     YVVSATGISLPYYVNLASLLLLVGMTWYLVMSVRLFLAMVPVAALMVYGLWILKINAM
                     PVAVICGAIWISAWIGQFIGHKIEGKKPSFFKDIQFLLVGPFWLMGKLFKKLGIAY"
     gene            complement(4367..5158)
                     /locus_tag="PKK76_03025"
     CDS             complement(4367..5158)
                     /locus_tag="PKK76_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNL67466.1"
                     /translation="MDVSHLFIWESRVLFLAKGLRAEAHAHYNASLIISADKPFEIRT
                     PERGYGGLECVLISPNVYHELRAEKSSMIVLQIDPLGAEYRALANALGGEGIIQAQRP
                     DGIEKELSILLDPASSRCESAAAFVRMLLMRTIHYEEMPHTVDPRVARLIALLKEHIS
                     EDMPETVSVQEAAAHVGLSADRLMHLFKEEMGLPIRRYVLWLKLRRAVMLMRTEGIRM
                     TEASHAAGFADSAHLSRTFKDMFGIPPSFFFANDRFVQVNQCEVW"
     gene            complement(5162..7078)
                     /locus_tag="PKK76_03030"
     CDS             complement(5162..7078)
                     /locus_tag="PKK76_03030"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNL67467.1"
                     /translation="MSAAEVFRILKRTWERFNEDGCYYRASAIAYALVLSAIPLMTIL
                     VKFAQVDQETIRAYLSRFMGAYGIQDSSEFLHALDEILSRADSIALVGVIFMIISATS
                     ILRHLEDSFNHIARSHVERPLHYRFAFYISGLILVPAFTTVTAGTMQYFLSQLKPPEW
                     KSMTLNDDTLWMLGKKLAWKQAKGEGSFHLETKIDKHAPYKDVYFDLQTGRSGRSWEI
                     LGEVPPRSLEAKDIRLLSRIDSADGVLYILGETGAVYFSNDNGQTFDYRLFAFKGASG
                     LRRPIFEDLLALPGKRALVLATVGSRSCLVDFKENAYGMQCFSTVYNNIVQIPNGKKS
                     EIYITGTARVLRSDDGGVTWNGPFDERYGSRTLMINTMARDGKGQTYFGGGSLWIRDQ
                     KGQLFLPELRAEHDIIGMHIDKDGQGFVYGDDELFRYTNDAGRTWYAPSNRILKNTTF
                     YSHQVLPNGDVVLAGENETYVLLGEPHVTMERDKSGHAFVEFQIKQEDHASLVVAFLL
                     RATLWMILYGIVFGLLSLGYKYIPTHPISRESAAVGGLFTATTLIGFVIGFQVWVTGF
                     ANTGRLYGVWAVIPVGMILLIFCTQILFFGLELACVLDERRTQDAYPEAGGVRLSVPL
                     KVEVPLVRAKKPRK"
     gene            complement(7075..7746)
                     /locus_tag="PKK76_03035"
     CDS             complement(7075..7746)
                     /locus_tag="PKK76_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67468.1"
                     /translation="MERRLVTGLALAVFVASGLFLLYKVRSEEQDFLGYQGRTAISTN
                     SIFDPALSPELAIDSDRKTFWKESMPGVQGTCTNETRTAPESDTRPPCLFLLIDAGFS
                     HRPGRPPEPDMPVSLTLEPGPAEYARPRAVRVVFFDQEIVDMDREFRLPTSPVFLAEK
                     SFVLSDGINHLPLDFLPPARVTAVYPGSVRKLWVRVEIESVYPGSKYSNTVGISELFV
                     ERKKQ"
     gene            7848..9155
                     /locus_tag="PKK76_03040"
     CDS             7848..9155
                     /locus_tag="PKK76_03040"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNL67469.1"
                     /translation="MSFPVSISLSQAGISFAAIAWMAGIVARSDVPRFPWPRLLIAGL
                     CIYGLELLSLILLSFSGGGFAFIKQGLSREIKDVFLILMAFWVFAFSEDEDRRKTIEK
                     LTYIAIGILIVSGLIGIFSRYRLSNIPYHLMHGWEGNMQARYQHHAGTFLAGTPLMFH
                     IYLPVGFLHSHLTYGALLMMAMPILFLRAMNPIIRYTSDSKKQTLIAIMIFGAACVVF
                     MINNARSAILGTIFVIALTVYYYSRFHWKKKALYLLIPAVIALLAFGGLFIFADLLHE
                     RLRALVIALSGQQKHTDYQRVLLWNASWEVIRSHPWLGVGAGHFQESVESILLNVSKQ
                     HPGLWYANETLQRGHSHSDPLHILAIAGIPAFISYIVFFAMLIHRAIKPAGRAIFRFG
                     APGLLIAGLYQCYFLDDAVLLPFWLLVGLTLRETETGQTSAKS"
     gene            9282..9839
                     /locus_tag="PKK76_03045"
     CDS             9282..9839
                     /locus_tag="PKK76_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67470.1"
                     /translation="MKLKIQTIITLVGFVFGLAATRTIQADSQCTNLSQSQCEKNDDC
                     TWVNGYTTQAGVKVDSYCRAKPGKANDQGDRDRRDRDKDKNRDKDKDKNRDKDKDKNK
                     DKDKDKRDKDKDKDRNKDKDKNRDKDKDKNKDKDKDKDKRDKDKEKRDKEKKDKEKKD
                     KERKDKEKRDKEKKDKDKKDKDKNK"
     gene            9976..>10437
                     /locus_tag="PKK76_03050"
     CDS             9976..>10437
                     /locus_tag="PKK76_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67471.1"
                     /translation="MDVRTLYLSLSLTALTGLLISWLSIHRDRRQGMQWNLGAIALCL
                     GNLLVSMRGTIPDLLSVNGANTLLLLVAPLIEASLQRFLRGRRISRLMLYWSLAAMIV
                     FNATAHLASLRERIQLMSLAFSGQCFLLAWVATNMPRDVRSRVVIVFGVAFG"
BASE COUNT         2779 a         2615 c         2532 g         2511 t
ORIGIN      
        1 tttgaagcca aagccagtgg cctgacaatg attgagcatc accgcgcccg cctgcaccgc
       61 cgtttttaca aaagcaagcg ccagcctttc aggatatgta ctctggtaat cgtaatatac
      121 gaatccgcct ttcaagcctt tcgactcaat ctttggttcg agcttcaaaa actcttctct
      181 ggaaatccat tttgcaggtg gaatctgttt gtcttctgga gttcccagat tgcggtcata
      241 cgccagaaga tcataagtgg aaagacccat ctgcatgatg aatcgacccg gattgctcca
      301 ttcaaacatg ggaacaagaa attgcatggg ccgactgagg tggcccgcgg ccagtcccat
      361 gatgtgacgc tcagacagag actcgcgcac taggccaaat tcgtaactct ccagatatct
      421 cagaccgccg tgaagcagtt tggaagtggc agaggaagtg gcgcaggcga aatcattttt
      481 ttcaacgcag agcacgcgaa ttccgcgaac ggcagcttcg cgagcaactg aaatgcccgt
      541 tatgccgccg ccaatgacga caaggtcaaa tttcttttcc aggtcgtgga attcgcgctt
      601 atgaatcacg cgccgtagcc tttctttttc aacagaatgc gacgctcaat ttcaaagatc
      661 ttttctggga tcttctcttt gcccatgtct ttcttatcgt tctctttgat gaagagatct
      721 gcaccgtgct tatcaaacgc ttcttttgtt ttgacgttct ttactccaat caattgaaca
      781 tgaagaatgc cagtgtttgg catggggcct tcttccttgg caacaatgcg cgagatggaa
      841 cctacagtgt cccctgaaat agccggagcg gtgtggtaac cttctgtgta gcccagatcc
      901 cagagcatat tctctgtaag atcgcgactg gccagaccgc ataaccatcc gaagacgagt
      961 ccgccatata caatcggttc tccgctcatt ggaccggaga gacctgctga atagagacga
     1021 tcgtagtgaa gtggatgtgt attgcccatg cgataggtcc atggcacatg ctcatccgtg
     1081 atggttcgca tgttcttgtg caccaggatc tgtcctgctt cgaaatcttc gaagtatgta
     1141 ttggacagag tgtgatgctt catgtcggtg gaaagagact tagcgtccgg cagttcgcag
     1201 gatgggtctt tgactaccgg gaaaggaaga tttgcatctc cgcgctttgg agcggattta
     1261 ccgtctcctg gtgggatcat gatctttctt tcatattgga tgacaacttc cccgcgctgg
     1321 tttagaccca gggtgttgac atgaacaatt cctggctttc cttctccgcg tactttcttg
     1381 gaaagcactc tggtgcggcc tgtgagtgta tctcctggat aaactggttt caaaaattga
     1441 acgttgtaat accccaggtt tgcgattgcc ttctctgaat tgttttgaac gcccagggag
     1501 agaatcacat tgaacacttg cagaggatga accagcatat ctgcaaatcc gtgcgcctgc
     1561 gcgtaagggg cagacaggaa gagaggagaa gattcgtgaa atactgttgc gaattcctgt
     1621 gcaaatgtgc gatcaatggt aaatgcacgc ggatgaagga acacatcgcc gtcgacgaaa
     1681 tcgtccagtg tgcggccgta ttctttctga atggatttag aaatgtccgc cgggtgagaa
     1741 ggagccagtt ctggaaatga attgagtgga aactttgcca taaccaggtt ttcgcctgca
     1801 agcggcgcga tcaagtgaaa gccgggttac tttttcctgg ccagaaatgt gtccatttcc
     1861 tcagaagaga agaacacctt attgcgggag ccaatctcgg tttgcgacac aatggaacca
     1921 aggtgcttgc ctgcgagaac aagaaccttt tctccgccta gaacggttac aaattgatcc
     1981 aggatgcgca attcagtcgg aggaatctct ggcacctgcc ggatatccag gatcacaatg
     2041 tccggcttga tcagctttaa aaagaaatgg ttgaatacaa cttttgcttc tggcagcgca
     2101 aagtctttca gattagagag aacggtcacc tgagtgcgcc cgcctttgcg gctaacgttg
     2161 atgtggttgg ctctcttgag ctcctgctgc agccgcaact tttcagccac agcgacggcc
     2221 ctttccacac gaccgcgaag gtccgcagcc tgcatggaag gcggaacaat gtccagaact
     2281 gccagctgct tgaggcgaag gatcagacct ttttctaacg gtgcgctgat caggcagatg
     2341 ccgatctctc ttgttgtatg ttcttttctc agattgctga tcacggaaat ggaatccgtg
     2401 cttttctcag tggcacccac cataatcaga tccggggaac cactttttgc aagctcgaga
     2461 ccccgagcta gattggatgc tgtaagaagc cgaacttcag agccttgcat tgcatgctca
     2521 aacttcgaga cttgatttgg atctgcatcg atcaagagca ccagggacac atgcccttcg
     2581 tacttgagga cggaacaacc gcaagttaaa aaatgagagg tgggctgaag tagattctgt
     2641 acttcggatt tcaggagcaa aacactggaa ccgatcgtac ttaccatctg cgcgggcgta
     2701 ggctttctgg cactgctctg tgcctactac ttctactccg tttctcataa accagttttg
     2761 cattttcaag aaaatgaatt tcatgatcag attttgagag aagtccctgt cctcacccga
     2821 gcctaccatc ctacgccctg gtgcttcaac aatcacctga tgcttcttct gttattgttc
     2881 agggaataca gatccaaaga ctatccgttc gacagaatag accagcttcg aatgaaggat
     2941 ggaggcatca ctggcctggc atggtctggc tggaacgaac agaacgctct tctgaaaaac
     3001 aagaagaaaa gcatcatgcc gatcgtagtg atctttcata caatcagcgg agatgaacag
     3061 gacgtaaaag gaattgtgga gtatgtacgc gtatccctgc gttggactgc agtggtctgc
     3121 atcaggcgag gacacggaaa ccttccgctt ggaaaaccaa agatcaacac aatgggttcc
     3181 acatctgacc tttcagagca acttgcattc attcagaaaa aattccctgg agcaccgtta
     3241 ttcggcattg gaatttcagc cgggtcaggt ttgcttgcca gatatctggg cgagtccggc
     3301 aagaagagta aattcatggc tgccgcagcc atttctcccg cttacgacat tgaaaaagca
     3361 tttcatcgca ttcatccagt ctacagcaaa atcatgggcc aacgattgat cagctatttt
     3421 ctaaagagac attatgacag cctttctaga gtgcacggct tcgatcatgc aatgcaggcg
     3481 caaaccattg gcgagtttca ggacagaatc catgcaatcg caggatacaa aacaaaagaa
     3541 gaatactacc gattttcgaa tccgatcaaa gtggcaaaga acattcgcac tccacttctc
     3601 atactgaact cacaagatga tcccatttgc gtaaatctaa acgtgcttga gaacttgcac
     3661 tggctcgaga atcttccaca cactatgctc gttcacacga agagaggaag tcatgtcgct
     3721 tatttcgaag gatggaaggc gaagccctgg tgcgaccggg ttgtgggaga ctacttccag
     3781 gcagttctaa aaactgaggc gcccactgcg aaaaggcgcc catcgaaaaa gaaacaagtg
     3841 cgaaggtaat cgtttgtgag aacgctcagt acgcaattcc cagtttctta aatagctttc
     3901 ccatcagcca gaaagggccc accagcaaaa actgaatgtc cttgaagaaa gaaggcttct
     3961 ttccttctat cttgtggcca atgaactggc caatccaggc agaaatccag atcgctccgc
     4021 agatcactgc cacaggcatc gcgttgatct tcagaatcca gagcccatac accatcagcg
     4081 ctgccacagg aaccattgcc agaaacaatc tcacggacat caccagatac caggtcatcc
     4141 ctacgagcaa aagcaagctg gccaggttca catagtatgg caaagaaatg ccggtggctg
     4201 aaaccacata gtcagaggca gcccagaaca atccaagagt actgaacata atgagaggaa
     4261 tgcagatcca gtgtatggtc tggttggtct catttctgtg gctttctgcg tattcatcga
     4321 accattgttg aacgtttttc atgatcataa cccccgatga tgaactctac cacacttcgc
     4381 actgattcac ttgaacgaat ctgtcatttg cgaaaaaaaa tgaaggagga atgccaaaca
     4441 tatctttgaa agttcggctc aagtgcgcag aatccgcaaa accggccgca tgcgaagcct
     4501 ccgtcatccg aattccctct gtgcgcatta acatgactgc ccggcgaagc ttgagccaca
     4561 gcacatatct gcgaatgggc aggcccattt cttccttaaa caaatgcatc aaacgatccg
     4621 cagacagtcc aacatgagcg gccgcttcct gcacggacac agtttctggc atgtcttcgc
     4681 tgatgtgttc cttgagtaag gcgatcaacc tggccacccg tggatctaca gtatgaggca
     4741 tctcttcgta gtgaattgtt cgcatgagaa gcatgcgaac aaaggccgcc gcggactcac
     4801 agcgggaaga ggccggatcc agaagaatag aaagctcctt ttcaatgcca tcgggtcgct
     4861 gtgcttgaat gatgccctct cctcccagtg catttgcaag cgctctatat tctgcaccca
     4921 gtggatcaat ttgcagcaca atcatggagc ttttctctgc gcgaagttcg tggtatacgt
     4981 taggcgatat taaaacgcat tcgaggccac cgtagccgcg ctcgggagtg cgaatttcaa
     5041 aaggcttatc tgcggaaata atcaaagagg cattgtaatg agcgtgcgct tctgcgcgaa
     5101 ggccttttgc gagaaacagt accctgcttt cccagataaa gagatgcgaa acgtccatga
     5161 ctcacttgcg cggtttcttc gcacgcacga gtgggacttc cactttcaga ggaacgctga
     5221 gtctcactcc gcccgcctcg ggatatgcgt cctgcgtgcg acgctcatct agaacacagg
     5281 caagctccag cccaaagaaa agaatctgag tgcagaaaat cagaaggatc atcccgacgg
     5341 ggatcacagc ccacactcca tagagtctac ccgtatttgc aaatccagtc acccagacct
     5401 gaaatccaat tacaaatcca atcaaggtgg tggcagtgaa gagtccaccc actgccgccg
     5461 actctctgga aattggatga gtggggatgt acttataacc aagagaaagc agaccaaaaa
     5521 cgattccgta gagaatcatc cacagagtgg cgcgaagaag aaaggcaacc acaagagacg
     5581 catgatcttc ctgtttgatt tgaaactcaa cgaatgcatg accgcttttg tctctttcca
     5641 tagtcacgtg tggctctccg agtaacacgt acgtttcgtt ttctccggcc agaaccacat
     5701 cgccgtttgg taaaacctgg tgcgaataaa aggtagtatt ctttaagatt cggttggaag
     5761 gagcatacca ggtgcggccc gcatcattgg tgtagcgaaa caattcatcg tctccgtaca
     5821 caaaaccctg cccatctttg tctatgtgca tcccgatgat gtcatgttcc gcgcgaagct
     5881 ccggaagaaa gagctgtcct ttctgatcgc gaatccagag agatccgcct ccaaaataag
     5941 tctgcccttt cccgtcacgc gccattgtgt tgatcatcaa agtacgcgaa ccatatcttt
     6001 cgtcaaaggg cccgttccac gtaactccgc cgtcgtctga gcgcagtacg cgagctgttc
     6061 cagtgatgta tatttcgctc ttctttccat tcgggatttg gacgatgttg ttatagacgg
     6121 tagaaaagca ctgcatgccg tatgcattct ctttgaaatc aacgaggcaa gaacgagaac
     6181 cgactgtggc aagaacgagc gctcgttttc cgggcaaagc aagtagatcc tcgaaaatag
     6241 gacgcctgag cccgcttgcg cccttgaatg cgaagagtct gtagtcaaac gtctggccat
     6301 tgtcgttcga aaaataaact gctccggttt ctcccagtat gtagagcacg ccgtctgctg
     6361 agtcaatgcg agaaagaaga cgaatgtctt ttgcctctag agagcgaggc ggcacttccc
     6421 ccagaatttc ccaggatctt ccagagcggc ccgtctgcag atcgaaatag acatccttgt
     6481 aaggtgcgtg tttatcaatc tttgtctcaa gatgaaacga tccttccccc tttgcctgtt
     6541 tccacgcgag tttcttgcca agcatccaca gagtatcgtc gtttaacgtc atggatttcc
     6601 attcgggtgg ctttaactgg ctgagaaagt attgcatggt cccggcagtc actgttgtaa
     6661 atgcaggaac aagaatgagc ccggaaatat aaaaagcaaa tctatagtgc agcggacgct
     6721 ctacgtggga acgagcaatg tgattgaatg aatcttccag atgccgcaaa atgcttgtgg
     6781 cggaaatgat catgaagatc acaccaacga gcgcgatgct gtccgcgcgg ctcaggatct
     6841 catccagagc gtgcaagaat tcagatgaat cctgaattcc atacgctccc ataaatcgcg
     6901 acagatacgc acggatggtt tcttgatcca cctgcgcaaa tttcacaagg atggtcatca
     6961 gtggaatcgc agatagaacc agagcatagg caatcgcact cgcgcgatag tagcagccgt
     7021 cttcgttgaa gcgctcccag gttcgcttca aaatgcgaaa gacttcagcc gcgctcattg
     7081 tttctttctc tccacaaata gttcagaaat accaactgta tttgaatact tagaaccggg
     7141 atacacagat tctatttcaa cgcgaaccca gagctttcgc acagaccccg gatacactgc
     7201 agtcacgcgc gcgggcggca gaaaatccag aggcaaatga ttgattccat ccgaaagaac
     7261 gaaagatttc tctgccagga aaacgggaga tgtgggaagc ctgaattcgc ggtccatatc
     7321 cacgatttct tgatcaaaga acacgaccct cactgcgcgt gggcgagcat attcggcggg
     7381 gcctggttcg agagtcaaag aaaccggcat atctggctca ggaggacgcc cgggtctgtg
     7441 cgaaaatcca gcatcgatca gaagaaacaa gcacggcggc ctcgtgtcag attcaggagc
     7501 tgtgcgcgtt tcattcgtgc aggtgccctg gactcctggc atagactcct tccagaaagt
     7561 cttcctgtca gaatcaatgg caagctctgg actcagagca ggatcaaaga tagaattggt
     7621 gctaatggca gtgcgcccct gatagcccag gaaatcctgt tcctcgctgc gcactttgta
     7681 tagaagaaac aaccccgagg caacaaacac agcgagcgcc aaaccggtga ccaatctgcg
     7741 ttccatattt ttgctgacag ttcccctgat tgattcagac gcaaacaata ttgaaagatc
     7801 gaagtctttt ttaccgaatc gcctggtact gcggagcagt atccgttgtg agctttcctg
     7861 tctccatcag cctttcccag gccggaatca gcttcgcggc gatcgcctgg atggcaggaa
     7921 ttgtggctcg ttcggatgtt ccgcgtttcc catggccgcg tcttttgatc gcagggctct
     7981 gcatctatgg tctcgaactg ttgtctctga tccttctttc cttttcggga ggcggatttg
     8041 cattcatcaa gcaagggctt tcgcgcgaaa tcaaagacgt atttctcatc ttgatggcat
     8101 tctgggtttt cgctttttca gaagacgaag atcggcgaaa gaccattgaa aaactaacat
     8161 acattgcgat tggaatcctg atcgtttcgg gactcattgg catcttcagc agatacaggc
     8221 tcagcaacat tccttatcat ttgatgcatg gctgggaagg caacatgcag gcaagatacc
     8281 agcatcatgc gggaacattc cttgcgggca ctcctctgat gttccatatt tatctgccag
     8341 ttggtttcct tcattcccat ctgacgtatg gcgctttact gatgatggcc atgccgattc
     8401 tgtttttgcg cgcgatgaac ccgatcatac gatacacttc agattcaaag aaacagactc
     8461 tgattgcgat tatgattttt ggtgcggcgt gcgtggtttt catgatcaac aacgcaagat
     8521 ccgccatcct cggaactatt tttgtcattg cgctgactgt ttactattac tccaggtttc
     8581 actggaaaaa gaaggctctc tatctgctga tcccagcggt gatcgcctta ctggcgtttg
     8641 gcggactttt tattttcgcg gatctcctgc atgagagact tcgcgctctg gtgatcgctc
     8701 tgtccggaca acaaaagcac acagactacc agagagtact gctctggaat gcttcgtggg
     8761 aagtgatccg gtctcatcca tggctgggag ttggcgcagg acattttcag gaatcggtag
     8821 aatccattct gctcaatgta agcaaacaac accctggtct gtggtacgcg aatgaaactc
     8881 tgcaaagagg acattcgcac agcgatccac ttcacatact ggcgatcgcc ggaatccctg
     8941 cttttatttc ttacattgta ttcttcgcaa tgctcatcca cagagcgatc aaaccagcag
     9001 gccgggccat ctttcgcttt ggcgcgccgg gactactcat cgcaggcttg tatcagtgtt
     9061 actttctgga cgatgcggtt ctactgccat tctggcttct ggttggcctg acgctgagag
     9121 aaaccgaaac cggacaaaca tcggcaaaat cttaaatttc taaacggccg cgaacaaaaa
     9181 cctgatcgct tcctgcaagt gatcgccaca aaaaagggtt gaaccctggg ctttgacgcg
     9241 atgtctaagg cttagtaata cgtttcagga aggttccttt catgaagtta aagatccaaa
     9301 cgatcatcac cctcgtcggg tttgtattcg gtctggctgc aacgcgaacg atccaggcag
     9361 attctcagtg caccaatttg tcccaatcac agtgcgaaaa aaatgacgat tgcacctggg
     9421 tgaacggata caccacgcag gcaggcgtaa aggtagattc atactgccgc gcaaagcctg
     9481 gcaaagcaaa cgaccaggga gaccgcgacc gtcgcgaccg tgacaaagac aagaaccgcg
     9541 acaaggacaa agataaaaat cgcgataaag acaaagacaa gaacaaagac aaagataagg
     9601 acaagcggga caaggataag gacaaagacc gcaacaagga caaagacaag aatcgcgata
     9661 aagataaaga caagaacaaa gacaaagata aggacaagga caagagagat aaggacaaag
     9721 agaagcgcga caaagagaag aaagacaagg aaaagaagga caaagaaaga aaagataaag
     9781 agaagcgcga caaagagaaa aaagacaaag ataagaaaga taaagacaag aacaagtaag
     9841 agttccttga tccaacgcgg ccggttctcc ggccgcgatt tcgttccaac agtcggaaat
     9901 catttgaccc ccgttccata gatgccatga tgaacgcgca cctctgcacg cgtcagagca
     9961 tgcgcaaaca gctagatgga cgtacgcaca ctttacttat ctcttagcct cactgcattg
    10021 acgggtttac tcatctcctg gctctccata cacagagacc gcaggcaggg aatgcaatgg
    10081 aatctcggag cgatcgcatt gtgtttgggt aatttgcttg ttagcatgcg cggaaccatc
    10141 ccggatctac tgtcggtaaa cggtgctaat acgctcttgt tgctggtcgc gccactcata
    10201 gaggcgagtc tgcaaagatt cttgcgcggt cggcgaattt ctcgattgat gctgtattgg
    10261 tcgctcgcgg caatgatcgt tttcaatgca acggcgcatc ttgcttcgct acgggagcga
    10321 atccagctca tgagcctcgc cttttccggc caatgtttct tgctcgcgtg ggtcgccaca
    10381 aacatgccca gggacgttcg atcgcgagtt gtaatcgttt ttggggtagc gtttggc
//
LOCUS       DAORFF010000043        10429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2915_length_10429_cov_3.673703, whole genome shotgun sequence.
ACCESSION   DAORFF010000043 DAORFF010000000
VERSION     DAORFF010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10429
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2915_length_10429_cov_3.673703"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PKK76_03055"
     CDS             <1..846
                     /locus_tag="PKK76_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNL67472.1"
                     /translation="PSVRYELYRDEFPDFRFRSYSEFGNPEKRRIDYTNASLFSSIVL
                     ARISQAEFRFRAGGAKEERMPTFLELFGERGSVIGNSNLKPEKSESSEGGFSLVADRQ
                     PMSGEFSITLFRKNIRDMILFVPNSQFSLRPENVDAARITGVEASAKMFIMKRLRLYA
                     TYTYQRAINMSDVSYLKGKYLPLRPLHDLHSGLSIYAGKWEMGAEADFTGAVFRDRTN
                     EYLSYQAGRWVYNAYLTFAPLGRGERELIFGLEMKNVLDQRMEDVIGYPLPGRTLYGT
                     INYKF"
     gene            821..1990
                     /locus_tag="PKK76_03060"
     CDS             821..1990
                     /locus_tag="PKK76_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67473.1"
                     /translation="MERSTTNSRFSFRIALPWLGVILVSFAVSGCKDIERPPLWTLFS
                     ASTGSARVGVVTTDFGSNGRFSVITTEGFPLPGLSPIYSDAVARYQNNQVTVINRLGR
                     DNIQVLEPAIGLLTVQEFSLGSGSNPHDYIRLSDAKGYAALYNRAYVQIVQPATGFLI
                     GTISLAAYADVDGIPEADSMLQEGSSVYVALERLDRNQTNLPPVGVSKLVEINSGSDA
                     IVAAYDFPSANPFGKMRRVVLGGVPHLVVPCPNRLGFLSAMDGGVVAFNLNTRTFRPG
                     FLYAETTAGGDILDVEIASDTVGYATVLDAVFNKSLQKFNPTTGQKLAVLSFSPSSAG
                     TISGLFLAPNGYLYAGDASFSQPGVRIYDTQRNDALVTPVPVSVGLRPFDFVYLP"
     gene            2041..2730
                     /locus_tag="PKK76_03065"
     CDS             2041..2730
                     /locus_tag="PKK76_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67474.1"
                     /translation="MMRVDRDSLRFSMSACFALAYLFSPGLFAEIRVDSSSQVFYKNA
                     TAMLGRMKDSPDPEIRKLYQEVSRASVTVHIRPITDDSRTWHVDGDRTRAHTEALDNL
                     GRGRARNRPTSAAIYLTTESVDPTRTSWKGGVLPHEMTHAMDLASGRYNKDYKIRERR
                     AIVFQNIWRARVNKTLREDYHGSFDTLDYQNAKRAGTLGKLTEHIFSSSDLPSSRDSN
                     DEGDDDDGDEE"
     gene            complement(2749..3744)
                     /locus_tag="PKK76_03070"
     CDS             complement(2749..3744)
                     /locus_tag="PKK76_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67475.1"
                     /translation="MGIREDFNHSRYIPGQKKGHYESFFQRANHPTRPLAFWIRYTVF
                     SPENDPASAIGELWAIYFDGEKNSHIAVKKEVPIRECEFDRNRFGAKVADAFLNQSEL
                     AGQVKQGKNSISWNLSFGSDAQPLFDFPESMYSTALPKAKVLVGMPLARFDGSLTVNG
                     QKVQVKSWLGSQNHNWGSKHTDHYAWGQVAGFDHSEDTFLELATAKLKFGPIWTPALT
                     PIVLRHRGQEFRLNQLIKTMGRASFGFFDWDFHASSAEIKIKGRITAKREDFVCLKYY
                     NPPGGFKYCLNSKIARCDLEILSSSSSHAEMLTSKSRAAFEILTDSNDHGMSPAC"
     gene            3859..5895
                     /gene="tgt"
                     /locus_tag="PKK76_03075"
     CDS             3859..5895
                     /gene="tgt"
                     /locus_tag="PKK76_03075"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNL67476.1"
                     /translation="MNGRLDFQLEAEASDSHARAGRFRTLHGEILTPLFMPVGTNATV
                     KGLRFEDLAQSGSQILLANTYHLLLRPGPEVFQTIGGIHNLTHWRGSFLTDSGGFQIF
                     SLSHARSMSEEGAAFRSYVDGRMIVLTPELSIATQKAIGSDIMMVLDQCIPSTADFET
                     AKRAMELTHRWARRSLNARGESPQSMFGIVQGAMYSELRRQSAEVLTQMEFDGFAIGG
                     LAVGETKAQREEFTEFTASLLPRDRPRYLMGVGTPIDLLEAVHRGVDMFDCIIPTALS
                     QQGVAYTSRGKMRLNRTVYKFAEEKLDPACDCYTCRTYSLAYLHHLKKSSELLGWQLV
                     SIHNLHFYHRLMASMRTHILAGTFRTFYEKAREELVREDEERPKGPRPRVRSTKNYES
                     RGAFSVHRTREGSMSIKDSASGEIMHPGADPGIEADRLYVNQSRLADRLREEGKEESE
                     LVIWDVGLGAATNAMAAVRAWELVQSENENVRRLRIVSFENDLDALNLALLHTGNIKH
                     LRHSAPREISMNGVYRSENGRLIWELLHGDFLELLGSAVVPDLIYFDPFSYKTNPSLW
                     TLETFARVFQRCSEHDTELFSYSASTRVRSALLAAGFYVASGLSSGEKSETTIAMTEQ
                     ALLRRKGSTEDELHLLNERWLERWQRSDAPYPLLLTVDEEPGVRSLILNHKQFR"
     gene            complement(5949..6290)
                     /locus_tag="PKK76_03080"
     CDS             complement(5949..6290)
                     /locus_tag="PKK76_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNL67477.1"
                     /translation="MKLITAMVQPHKVEDVKQALYKAGVTKMTVLNAMGCGQQKGYDE
                     NYRGAVHTVNLLRKVVFEIAVNEDYVEPTIKAVIEGARTGKIGDGKIFVTELKEVIRI
                     RTGERGKEAIG"
     gene            complement(6308..7849)
                     /locus_tag="PKK76_03085"
     CDS             complement(6308..7849)
                     /locus_tag="PKK76_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNL67478.1"
                     /translation="MKSDFKKWSYFLVLGAVLVSARLYAETPAAPVPAGPTVDQRLAD
                     LEAYINNGAVGAAGTAKWTSNVPGSGPGHNGWMMVCSALVLFMTLPGLALFYGGLTRQ
                     KNILSVMAQCLGIAGVVTILWWAAGYSLVFANDSPTDATGALTPIVTLFGSLKHAFLR
                     GVDSVPNTDYAYWVSHNVFSMYQLMFAIITPALIIGAIAERMKFSAIIIFVTLWMFAV
                     YFPLAHAVWGINGMMNGVWNSAAKIHAIDFAGGTVVHMSSGWSALILCIILGKRIGFG
                     TKAMDPHNLVLTMVGTGMLWVGWYGFNAGSAVAADGVSANAFMTTTLATAVASFVWPF
                     LEYIIKRKPTVLGFCSGAVAGLVVITPACGFVNASGSIIIGLLAGSIPFFAVYKLKGW
                     LGYDDALDTFGVHAVGGTMGALVTGFLAVPAKFADGKVNPGAVNANLASNIDAFINSG
                     TLWIEQLKAIGFTLVLSVVATAVIAYLIKFTIGLRPSEEVETLGLDISEHGEEGYRGF
                     QMWND"
     gene            complement(7995..9053)
                     /locus_tag="PKK76_03090"
     CDS             complement(7995..9053)
                     /locus_tag="PKK76_03090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNL67479.1"
                     /translation="MQRIDQFCAGFATGDAISNEALLLQKHLQKRGIKSQIFAEQFPD
                     RDAHRVVHIRKFKPKPDSILVYHHSTDTELLSRLPSLDCKKILIYHNVTPAEYVRPYN
                     RGLADRLQHARDVLKEIPHLFQTVLADSAYNASDLERMGYKDVKVMPVALSPIWKTNP
                     QRKNYRFTVLFVGRVFPNKRHQDLLKTFYFLKKIQPDARLRLVGHFHPEMRGYTGELK
                     NLMRELDLRDVEFTGMVSDEQVAQAYADADVFLSMSEHEGFFVPLIESMHFRLPILAY
                     NSSVIPETLGGSGVMFTEKNFPMVAETIVELHENSPFRESILEAQTQRAAAFSLSSTT
                     GIFDSALDQALQGDLPKL"
     gene            complement(9053..9496)
                     /locus_tag="PKK76_03095"
     CDS             complement(9053..9496)
                     /locus_tag="PKK76_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67480.1"
                     /translation="MQKKLRVNFFRAFFLIPAAAFLLPGCQKSDPVTPAVIEKYQADA
                     DLFCNAIVDCMKEEVREKMKATPERRDLVLARMTRDNCRKGQYSLIGRLSTDVAARGA
                     ADEAGIYREYAECAHAVAEAKDCTQRKQLQKEHPACAKMKAESGY"
     gene            9540..9953
                     /locus_tag="PKK76_03100"
     CDS             9540..9953
                     /locus_tag="PKK76_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacitracin resistance protein BacA"
                     /protein_id="HNL67481.1"
                     /translation="MTEMYVPPGGPPQGPPPSREILQTLGEPYIRKLLQEHYSQLEKS
                     AIRGMFPEDMTEAADRSADFFVQLLGGPPYFSQKYGPPRMRARHMPFAITEAAREIWL
                     GCFKRAMDSLEFPANYREEFERFLVEFSAWMVNRR"
     gene            10026..>10429
                     /locus_tag="PKK76_03105"
     CDS             10026..>10429
                     /locus_tag="PKK76_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67482.1"
                     /translation="MRKFCALWAITLVWSVTRCITTEEVPVARTDTPVRAEMIVKKVE
                     RHLCTTAWGGSCSFQESAILLHTGPVSTREFGYGCLLTRDGVPQAQVVTDKSDGIRIA
                     FRCARNLGWTVVYVGAENRSFKDCKLYNFPGE"
BASE COUNT         2453 a         2594 c         2735 g         2647 t
ORIGIN      
        1 ccctccgtgc gatacgaact ctatcgggat gagttccctg attttcgttt ccgatcttat
       61 tctgaatttg gaaatccaga gaagaggcga atcgactata ccaatgccag tttattttct
      121 tctattgtcc tggcccgaat ttcgcaggca gaatttcgct ttcgcgcagg cggtgctaaa
      181 gaagagcgca tgcccacgtt tctggagcta tttggcgaga gaggaagcgt gatcgggaat
      241 tccaatctca aaccggaaaa gtcagagagt tcggagggag gtttttccct cgtggcagat
      301 cggcaaccta tgtccgggga gttttccatc actctctttc gaaagaacat ccgcgacatg
      361 attctgtttg ttccaaattc acagttttct ctgagacctg agaatgtgga cgcagccaga
      421 atcacaggtg tggaggcgtc cgcgaaaatg ttcatcatga aacgactgag gctgtatgcc
      481 acatacacat accagcgtgc gatcaatatg agcgatgtaa gttatctaaa gggaaagtac
      541 ctgcctctca ggccattgca cgatctgcat tctggactca gcatttacgc gggaaaatgg
      601 gaaatgggtg cggaagcaga ttttacgggc gctgtgtttc gcgatcgcac aaacgaatac
      661 ttgagctatc aggcaggcag gtgggtatac aatgcctatc tcactttcgc gcctctgggc
      721 cgcggtgaga gagagctgat ttttgggctt gaaatgaaga atgtactgga tcaaaggatg
      781 gaagatgtga tcgggtatcc gcttcctggg agaacgctgt atggaacgat caactacaaa
      841 ttctagattc agctttcgca tcgccttacc atggttaggt gtgattctcg tatctttcgc
      901 ggtgagcgga tgcaaggata ttgagaggcc ccctctctgg actttgttct ctgcctccac
      961 tggctctgcg cgcgttgggg tggtgactac tgattttgga tccaacggta ggttcagtgt
     1021 aatcacaaca gaagggtttc cgctccccgg actttctcct atttattcag atgcggtggc
     1081 caggtaccag aacaaccagg taaccgtgat caatcgtctg gggcgtgaca acattcaagt
     1141 tcttgagcct gccattggtc ttttgaccgt tcaggaattt tctctagggt caggaagcaa
     1201 tcctcacgat tacattcgtc tttccgatgc gaaaggttac gccgcgctct acaatcgagc
     1261 gtacgttcaa attgtacaac ccgcaacagg tttcctgatc ggtacgattt ctctggctgc
     1321 gtacgcagac gtggacggaa ttccggaggc ggacagcatg cttcaagagg gaagttccgt
     1381 gtatgtggcg ctcgaaaggc tggaccgaaa tcagacaaat cttccgccgg taggtgtttc
     1441 gaaactcgtt gaaatcaatt ctggtagcga tgccattgtg gccgcatacg attttccgtc
     1501 agccaatccg tttggaaaaa tgcgtcgcgt ggttctgggc ggcgtgcctc acctggttgt
     1561 gccatgtccg aatagacttg gatttctttc cgcgatggac ggtggagtgg tcgcgttcaa
     1621 cttgaatact cgcacgtttc gcccggggtt tttgtatgcg gaaacaacgg caggaggcga
     1681 tattctcgat gttgaaattg caagcgatac cgtaggttat gcgactgtgc tcgatgcagt
     1741 gttcaacaaa tctttgcaaa agttcaatcc tactaccggg cagaagcttg ctgtgctttc
     1801 tttttctccg tcgagtgcgg gcacaatatc aggtctgttc ctcgcaccga acggttatct
     1861 ctacgccggg gatgcttctt tttcccaacc gggagttcgc atctatgaca ctcaaaggaa
     1921 cgacgcgctg gtcactcctg tgccggtcag cgtgggtctt cgtccgtttg actttgtata
     1981 tcttccgtga cagttgcaaa aatcgatcga ccgaatagac aacgcatccg tccaaagaac
     2041 atgatgcgcg tagatcgtga ttcccttcgc ttctcgatga gtgcatgttt cgcactcgct
     2101 tatctttttt ctcccggtct ttttgctgaa atcagagtag attcgtcatc gcaggttttt
     2161 tacaaaaatg cgactgcgat gcttggtcgc atgaaggatt cgcctgaccc ggaaatacgc
     2221 aaactctacc aggaagtgtc tagagcatcc gtgactgtcc acattcgccc gatcacagat
     2281 gacagccgca cctggcacgt ggacggagat cggacgcgag cccacacgga ggcgcttgac
     2341 aacctcgggc gcgggagggc gcgaaatcgc cctacgagcg cagctattta cctcaccaca
     2401 gagtcagtgg atcccaccag gacatcctgg aaaggaggtg tattgccgca cgagatgacg
     2461 cacgcgatgg atctggcttc cgggaggtac aacaaagact ataagatccg ggaacgccgg
     2521 gccattgtgt ttcagaatat ctggcgcgct cgcgtcaaca aaactctgcg cgaagactac
     2581 cacggatcct ttgatacgct agactatcag aacgcaaaga gggcaggaac gctgggcaag
     2641 ctcacggaac atattttctc aagctctgat ttgccctcct ctcgcgattc aaatgatgaa
     2701 ggggatgacg atgatgggga cgaggaatga tttcatggag cttctcttct aacacgcagg
     2761 agacattcca tgatcgtttg aatcggtaag aatctcaaag gcggcgcgac ttttggaagt
     2821 aagcatttct gcgtgtgaac tgctggagga taggatctcc agatcacacc ttgcgatctt
     2881 tgaattcaga caatatttga acccacctgg tggattgtaa tacttcaagc atacaaaatc
     2941 ttctctcttt gccgttatgc gacctttgat cttgatttcc gcagagcttg catgaaagtc
     3001 ccagtcaaaa aatccaaagc ttgcgcggcc cattgtcttg atcaattggt tcaatctaaa
     3061 ttcctgtcca cgatgacgaa gcacgatcgg agtgagggcc ggagtccaga tgggtccgaa
     3121 cttcagcttc gcagtggcga gttcgagaaa cgtatcttcg ctgtgatcaa agcccgccac
     3181 ttgaccccat gcatagtgat ctgtatgttt ggaaccccag ttgtgattct ggcttcccag
     3241 ccaggatttg acctgaactt tttgtccgtt gactgtgagt gacccatcga acctggccag
     3301 aggcatgccc accagaacct ttgcttttgg aagtgctgtg gaatacatgg actccggaaa
     3361 atcaaagagt ggctgtgcgt ccgatccaaa gctcagattc caggagattg aattttttcc
     3421 ctgcttcacc tggcctgcta gttcactctg gtttaggaac gcgtcggcca ctttcgcacc
     3481 gaaacgattg cgatcaaatt cgcactctcg gatggggact tcttttttca ctgcgatgtg
     3541 actgttcttt tctccgtcaa agtatatggc ccagagttca ccgatcgcag acgcaggatc
     3601 attctccggg gagaaaacag tatagcgaat ccagaatgcg agcgggcgag ttggatggtt
     3661 tgctctctga aagaagcttt cgtagtgacc ctttttttga cccgggatgt atctgctatg
     3721 attgaaatcc tctcgaatgc ccatgggatg ggaatgcacc gggccatcgt gtatgtcaag
     3781 gtttgcgggg aactcggcgc ggcctcctgt ctgctaaatt gactcgacgc ggggcattca
     3841 tcccagatcg attccatcgt gaacggccgt ctggattttc agctcgaagc agaagccagc
     3901 gatagccacg cgcgcgcagg cagatttcgc acgttgcatg gggaaattct cacacctctc
     3961 ttcatgccag tggggaccaa tgcaaccgtg aaaggtcttc gctttgaaga tctggcgcag
     4021 tcaggctcac agatcctgct tgcaaatacc tatcatttgc tgttacgccc gggcccggag
     4081 gttttccaga cgataggcgg cattcacaat ctaactcact ggaggggatc atttttgact
     4141 gactcggggg gatttcagat cttttccctt tcgcatgctc gcagtatgag cgaagagggc
     4201 gctgcgtttc gtagttacgt ggatggcagg atgattgtgc ttacgcctga actgagcatc
     4261 gcaactcaga aagccatagg aagcgacatc atgatggtgc tcgatcagtg cattccgtct
     4321 acagctgatt ttgaaaccgc aaagcgagcc atggagctca cacacagatg ggccagaaga
     4381 tcattgaatg caagagggga aagtcctcag tctatgttcg ggatcgtgca gggcgccatg
     4441 tattctgaac tccggcgcca gagcgcggag gtattgactc agatggagtt tgacggcttt
     4501 gccattggcg gtcttgccgt aggggaaaca aaagcacaga gagaagagtt cacagagttc
     4561 acagcttctc ttttgccgcg cgatcggccg cgctatctga tgggtgtggg cactccgatt
     4621 gatttgctgg aagctgtgca tcgcggtgtg gatatgtttg attgcattat tcctactgct
     4681 ctttcgcagc aaggagttgc ttatacctcc cgcggcaaaa tgcgcttgaa tcgaacggtg
     4741 tacaagtttg cggaagaaaa actggatccc gcctgcgatt gctacacgtg ccgcacttac
     4801 agcctcgcat atctccatca tttgaaaaaa tcatctgaac tcctggggtg gcagcttgtt
     4861 tctattcaca acttgcattt ctatcatcga cttatggcga gtatgcgaac gcacatcctg
     4921 gccgggactt ttcgaacttt ctatgagaag gcaagggagg agctcgtgcg cgaagatgag
     4981 gagaggccaa aaggtccgcg cccacgagtt cgttctacaa aaaactatga atccagagga
     5041 gccttttctg tgcatcgcac gcgcgaagga tccatgtcga tcaaagacag cgcttccggg
     5101 gaaattatgc atcctggcgc ggatccgggg attgaggccg atcgcctgta tgtgaatcag
     5161 tcaaggctcg cggaccggtt gcgcgaagag ggaaaagaag agtccgaact agtcatctgg
     5221 gatgtgggcc ttggtgcggc aaccaatgcg atggcggccg tgagggcgtg ggaactggtt
     5281 cagtcagaga atgaaaacgt gcgacgactt cgtattgtta gttttgaaaa cgatctggac
     5341 gcattaaacc tggcccttct ccatacaggg aacatcaaac acttgagaca ttccgcaccg
     5401 cgagagatct ctatgaatgg cgtctatcgt tccgagaacg gacgactgat ctgggaacta
     5461 ttgcacggcg attttctaga gcttctgggt tccgcggttg ttcccgatct gatctatttt
     5521 gacccgtttt cctataaaac aaatccgagc ctctggacgc tggaaacatt cgcgcgagtc
     5581 tttcaacgct gtagtgaaca tgacacggaa ctcttttctt acagcgcatc gaccagggtt
     5641 cgttctgctc tcctggctgc aggtttctat gttgcatctg gtttgagttc aggcgaaaaa
     5701 tcggaaacaa ccattgccat gacggaacaa gctttgcttc ggcgaaaggg ctcaaccgaa
     5761 gatgagctgc atttgttgaa tgagcgctgg ctcgaaagat ggcagagaag cgatgctcct
     5821 tatccgttgc tactgaccgt agacgaagag cccggtgtgc gcagtttgat tttgaatcac
     5881 aaacaattca ggtaaaaaaa aagccggccc ctgggggagg gccggctcat gggggatgct
     5941 tattttacct agccaatcgc ttctttgccg cgttcgcctg tgcgaatgcg gatgacttct
     6001 ttgagctctg ttacgaagat cttgccatcg ccaatttttc cggtgcgagc accttcaatg
     6061 acggccttga tcgtcggctc aacgtagtct tcgtttactg cgatttcgaa tactaccttg
     6121 cgaagcagat tcacagtgtg tactgcgccg cggtagttct catcgtagcc tttttgctgg
     6181 ccacagccca tcgcattgag aaccgtcatt ttggttactc ctgctttgta cagagcctgt
     6241 tttacgtcct caactttgtg aggttgaacc attgctgtaa tcaatttcat ggtgtttctc
     6301 ctctacctca atcgttccac atctggaagc cgcggtagcc ctcttcacca tgttcactga
     6361 tgtcgagtcc cagagtttcc acttcttcag acggacggag tccaatcgtg aatttgatca
     6421 gatatgcgat caccgctgtg gcaaccacgc tcagaaccag ggtaaagcca attgctttga
     6481 gctgctcgat ccagagtgtt ccactgttga tgaatgcatc aatgttggaa gcaaggttcg
     6541 cgtttacagc tccagggttt actttgccgt ctgcaaattt tgcaggaact gcgaggaagc
     6601 ctgttaccag agcgcccatg gttccaccta cagcatgcac accgaatgta tcgagtgcgt
     6661 catcatatcc aagccagcct ttcagtttgt agactgcgaa gaatggaatg gatccagcca
     6721 gaagaccgat gatgatagag ccgcttgcgt ttacgaaacc gcatgcagga gtgatcacaa
     6781 ccagaccggc caccgcacct gagcagaagc ccagaacagt tggttttctt ttgatgatgt
     6841 attcgaggaa cggccacacg aagcttgcta ctgctgttgc aagagtggtg gtcatgaatg
     6901 cattggcaga tacaccgtct gctgctacag cagatccagc gttgaatcca taccaaccaa
     6961 cccacagcat tccagtcccc accatagtga gcaccaggtt atgcggatcc attgctttgg
     7021 ttccaaagcc aatgcgctta ccaaggatga tacacagaat cagagcagac cagcctgaag
     7081 acatgtgaac cacagttcca ccggcaaagt caattgcgtg aatcttggca gcggagttcc
     7141 acacaccatt catcataccg ttgattcccc agaccgcgtg agcgagtggg aagtaaactg
     7201 caaacatcca cagtgtaaca aagatgatga tcgcagagaa cttcatgcgt tctgcgatcg
     7261 caccaatgat cagtgcaggc gtgatgattg cgaacatcag ttgatacatg gagaatacgt
     7321 tatgcgacac ccagtatgcg tagtcagtgt ttggaacgct gtccacaccg cgcaggaacg
     7381 catgcttcag actgccgaat aacgttacga ttggagtgag tgctcctgtc gcatccgtcg
     7441 ggctgtcatt ggcaaatacg agactgtagc ctgcagccca ccatagaatc gtgacgactc
     7501 cggcgatgcc aaggcactgt gccataacag agagaatgtt cttctggcga gttaagccgc
     7561 cgtagaacaa tgcgagaccc ggcagggtca tgaaaagaac gagagccgag cagaccatca
     7621 tccagccgtt gtggccagga cctgatcctg gaacgttgga tgtccacttt gcagtgccgg
     7681 ccgctccaac tgcgccgttg ttgatgtagg cttccaggtc tgccagacgc tgatctacag
     7741 ttgggccagc aggtaccgga gccgcagggg tttccgcata caatctagca ctaacaagta
     7801 ccgcgcccag caccaggaaa taagaccatt tcttgaaatc cgatttcatc gaatcctcct
     7861 gagtgattgc tgccctcttt atgcaaggtc ggtaccacct tcctattttc tgtgcatctg
     7921 cacctttgcc gctatttttg gcacaattcg gcagaggaag cagccgagga gcgtactctg
     7981 ggcgggcttt gtgcttaaag tttaggcaga tcgccctgaa gggcctggtc caatgcggag
     8041 tcaaagatgc cagttgtgga ggaaagggag aatgccgctg ctctttgcgt ctgcgcttcg
     8101 aggatggact cccgaaatgg gctgttctcg tgcaattcca caatggtttc tgctaccatt
     8161 gggaagtttt tttccgtaaa catcaccccg cttccaccca gggtttccgg gatcacagag
     8221 gagttgtacg caaggatggg gaggcgaaag tgcatggatt cgatgagagg gacaaagaaa
     8281 ccttcgtgtt cactcataga caaaaataca tctgcatctg cgtatgcctg cgccacctgc
     8341 tcatctgaga ccatgcccgt gaattccaca tctcgcaggt ctaactccct catgaggttc
     8401 ttgagctctc ccgtatagcc tctcatttct ggatgaaaat gtccgacgag tcgcaagcgt
     8461 gcgtccggtt ggatcttctt tagaaagtaa aaagttttaa gtaaatcctg atgacgttta
     8521 ttggggaaca ctcgacccac aaacagcact gtgaagcggt agttcttgcg ctgagggttt
     8581 gtcttccaga tgggagaaag tgcaacgggc atcactttca catccttgta acccattcgt
     8641 tctagatcag aagcattgta tgcagagtct gcgagaactg tctgaaacag atgcggaatt
     8701 tctttgagca catcgcgtgc gtgctggagt ctgtctgcga gcccgcgatt gtacgggcga
     8761 acatattctg caggcgttac gttgtgatag atcagtattt ttttgcagtc cagagaaggg
     8821 agacgcgaaa gtaattccgt atccgtggaa tgatggtaaa ccagaataga gtctggtttt
     8881 ggcttgaatt tacggatatg aacgactcta tgcgcatctc tatccgggaa ttgttctgcg
     8941 aaaatttgcg atttgattcc gcgtttttgc agatgtttct gcagcagaag tgcttcgttg
     9001 gagatggcgt ctcctgtcgc gaatccggcg cagaattgat cgattctctg catcagtaac
     9061 cggattcagc tttcattttc gcgcaagcgg gatgttcttt ttggagctgc tttctttgtg
     9121 tgcaatcctt tgcttctgcc actgcatgag cgcactccgc atattctctg taaattccgg
     9181 cttcgtctgc tgcaccacga gccgcgacgt ccgtactcag cctgccgatc aggctgtact
     9241 gtccttttct acaattgtcg cgagtcatgc gcgcgagaac caggtcccgg cgttctggag
     9301 ttgccttcat cttttcgcga acttcctctt tcatgcagtc tacgattgca ttgcagaata
     9361 aatctgcgtc tgcctggtat ttctcgatga cggccggtgt cacagggtct gatttctggc
     9421 aaccgggcag taaaaacgcg gcagcaggga ttaaaaaaaa ggcgcgaaag aaattcacgc
     9481 ggagcttttt ttgcattcat cgtaaatgcg ccgcaaagac catagcgcaa gcaggtttta
     9541 tgactgaaat gtacgttccg ccgggaggcc caccacaggg gccgcctcca tcccgtgaaa
     9601 tactccagac cctgggcgag ccctatattc gtaagttatt gcaggagcat tattcacagc
     9661 tcgaaaaaag tgccatccgc ggaatgttcc cggaagatat gacagaggcg gcagatcgat
     9721 ctgctgattt cttcgtgcag cttctgggag gtccgccgta cttttcgcag aaatacggcc
     9781 cgcctagaat gcgtgccagg cacatgcctt ttgccatcac cgaagcggcg cgcgaaatct
     9841 ggctcggatg cttcaagcgc gccatggatt cgctcgaatt tccggccaat tatcgcgaag
     9901 aatttgagcg ctttctggtc gagttttcgg cctggatggt caatcgacgc tgaatcccgg
     9961 atcaatcaaa accgattttg gaaacatctt gacactccct gaggcgagag cggccaatct
    10021 gcagaatgcg aaagttttgc gcgctctggg cgatcactct agtctggtct gtcacccgct
    10081 gcatcaccac tgaagaagta cccgtggccc gcacagacac tcctgtgcgc gcagaaatga
    10141 tcgtaaagaa agtagaaagg catctatgca cgactgcatg gggaggctcc tgctcctttc
    10201 aagagtccgc cattctcctt cataccgggc ccgtgagcac tcgtgagttt ggatatggat
    10261 gtctcttaac ccgggatgga gtcccgcagg cgcaggtagt tacagataag tcagatggga
    10321 ttcgcattgc ctttcgttgt gcgagaaacc ttggttggac tgtagtttac gtgggagcgg
    10381 agaacaggtc tttcaaagac tgcaagctgt ataattttcc tggcgaatt
//
LOCUS       DAORFF010000044        10429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2916_length_10429_cov_3.503952, whole genome shotgun sequence.
ACCESSION   DAORFF010000044 DAORFF010000000
VERSION     DAORFF010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10429
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2916_length_10429_cov_3.503952"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PKK76_03110"
     CDS             complement(<1..283)
                     /locus_tag="PKK76_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67483.1"
                     /translation="MKSWEPDQEFERADFPRAGKKPKDAPQKREATPGREVKAWKPRT
                     VSEAAATPRASSSAPAAKSQAPLAKNLDAQGGKSEPRVISSSSGYAAATL"
     gene            319..1920
                     /locus_tag="PKK76_03115"
     CDS             319..1920
                     /locus_tag="PKK76_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002744214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNL67484.1"
                     /translation="MDASSTEKPTSPLEREISRRRTFAIIAHPDAGKTTLTEKLLLYG
                     GAIQLAGHVRARRDRRKTRSDWMTMEQERGISISTAALQFEYNGYRMNLLDTPGHEDF
                     SEDTYRTLMAADCAIMLLDASRGVEPQTIKLFKVCRERGIPIITFINKMDLPAKDPFS
                     LLDEVEKVLGITAIPLLWPMGSGKDFRGVYDLVEKKVHRYERVAGGASQVPVTVGGID
                     DPTLDEIMDEYTARVFREGADLVQNALPPFSQEDFIAGTITPVFFGSAVTNFGVQLFL
                     NNFLTLAPHPPPVHLTDGTDLLPSSPVFTGFIFKLQANMNRAHRDRVAFLRITSGKFE
                     RGMTAFVPRLGKQVHLTNPVAFFGQERSTIDDAYPGDIIGMINPGTYHIGDILSTGKP
                     PAFPDLPRFAPDMFASLIPTDTSKLKQFKKGIQELAEEGVVQIFSTQDGSVVIGAAGQ
                     LQFEVFRFRMEDEYGAPCRLEVMPFECSRWIRPSDANKFSSYDRIVKDEKGRTVVLFK
                     SEYRLKSFLQTNADIPVYDHPPQAD"
     gene            1975..2520
                     /locus_tag="PKK76_03120"
     CDS             1975..2520
                     /locus_tag="PKK76_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNL67485.1"
                     /translation="MSDPNFFQTVVLMVEHNAEGAFGLVVNRRSRLVLSDILPGFAHN
                     RGASTPIYVGGPVQQEYLFVLHSDLPDAEAGSEYRSIPVKGVNFEPAFRRIENYFNEE
                     NWNAIPADDRPGIHLYLGYSGWAPGQLEQEMDRGSWIIHPASAKIVFHENPEEGWANA
                     LRDKGGIYRVFADTNPDPGLN"
     gene            complement(2521..4200)
                     /locus_tag="PKK76_03125"
     CDS             complement(2521..4200)
                     /locus_tag="PKK76_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL67486.1"
                     /translation="MSSPEEFLRKLEAQKLRFVIALSSVVFPVTVIQSIIRWNAGLKL
                     PALINCVLMVGALISLVLALKRKLNPAAHALALLASITTGLNQFVTSSGTIAPLLNVL
                     VLFAASVYMLPLKWGIFYGVLAFVRAITIRLLSDVWGIRGDSPIVPVTEWYTAMFVSF
                     FVFFYVMVARETSAHRLLDTYRQAAQTRARFLSRMSHEIRTPLNGVLGIGEVLGEKLH
                     EPEQKEYLRIMTNSGRHLLHIVNQVLELARTENRITLVRENYEPALVLNQVAELFRSA
                     LTSKDLGVHIEVDPALPRVVSGDEGSVRQILVNLMHNAVKFTEKGGISLRVRTDASGG
                     LERIIYSVEDSGRGISAEKVTSVFDAFEQGDVTTATADGTGLGLAICRELCAQMDGVI
                     ELRSKKSEGSAFIVSLPLFMPVTGVVPVESKTGIKSFPEGSVAVLYVEDDGVNRTLME
                     AFLKPPQFLLTVAPDGATAIESFSKKKFDIVLMDLHLPDMSGYEILSEMRKIENQIGQ
                     KHTPVIAVSASVIAEEVTRSLEAGFAHHLGKPISKSGLMAAMHRVLDPELS"
     gene            complement(4212..7022)
                     /gene="secA"
                     /locus_tag="PKK76_03130"
     CDS             complement(4212..7022)
                     /gene="secA"
                     /locus_tag="PKK76_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004758870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNL67487.1"
                     /translation="MFQKALTLIFGSKHERDIRKLLPIVEKINSLEPSLKALSNEELG
                     AKTAEFKARLARGETLDDILPEAFACVREASVRTLGLRHFDVQLIGGMVLHQGKIAEM
                     KTGEGKTLTATSAIYLNSLSGKGVHIVTVNDYLARRDATWMKPIYDLLGVSVGVIQHN
                     LSTEERKQAYNSDITYGTNNEYGFDYLRDNMVSEPEYRVQRPHNFGIVDEVDSILIDE
                     ARTPLIISGPTDENTDKYASIDKAIIRLIQEEDKAPEPPPREVEPGKEEPHVIFGFYY
                     DVDEKSRNVTLTEAGVHKMEEILQVENLYAPENVELVAHTTQALRAHLVYKHEVDYWV
                     REGEVMIVDEHTGRLMEGRRYSDGLHQAIEAKEGVEIRQETQTLASITFQNFFRIYNK
                     LAGMTGTADTESEEFKKIYRLEVVVIPTNVRVQRIDHPDRVYRSEREKFNAIVQELAA
                     LHAKRQPVLVGTISIEKSEMLSGLLKKAGIPHSVLNAKHHEREAEIVENAGVPGAVTV
                     ATNMAGRGTDIVLGGAARYLKDLEELEETDEASAKFRNAVLKKEFAEARNLISTLDTA
                     ARKDLAKAIADRAEIWLKSHKEVKELGGLHILGTERHEARRIDNQLRGRSGRQGDPGS
                     SRFYLSLEDQLMRLFGGQRLMNIMERLGMTEGQELEAGMVDRAIARAQKRVENHNFDM
                     RKHLLEYDDVMNRQREFIYAERNKLMSNENVRAHLREWMNEVIENKVLEFCEGSDPFR
                     WDLESLKEWTRTVLGLDLNLDADDYKRVRNPLETLYDHIVEKSIEHYDAHAASIGGEN
                     FSYIERRIALDVIDARWKDHLYAMDHLREGIWAISYSERNPLVEYKIQGFRLFDALVQ
                     LIKEQITEFLFRVEVQGPIQQTSRRQPVGQEQHQSLGSFESTSGPGVGPGGLPAASQK
                     APEITVSGGGASRRKSSRKRRR"
     gene            complement(7066..7734)
                     /locus_tag="PKK76_03135"
     CDS             complement(7066..7734)
                     /locus_tag="PKK76_03135"
                     /inference="COORDINATES: protein motif:HMM:NF025718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HNL67488.1"
                     /translation="MSYPRREIALGIFYQYSSYVIMQTFCLLAETTRGPTLPDRLHEW
                     IHADRTLAVYNSTIWLPGVLVFIAFLAIRSPMACVNYMRVGAVVTVFRGLFIFATSLG
                     PPLAVMSDAPPAMLSLTRESITLPLLIRQWIPLDVLYGGSGLSAAYLTQDLFFSGHTA
                     TTFLFCLVTRGWIRTVSVFFHIVTVALLILTHEHYTIDILGAYFVVYAVHSFFERRDL
                     LLSA"
     gene            complement(7731..9461)
                     /locus_tag="PKK76_03140"
     CDS             complement(7731..9461)
                     /locus_tag="PKK76_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67489.1"
                     /translation="MILTTRRHANIRVSMIWFHLQSFNRFRFALLALFALASFWVIGA
                     DPHTPVLEPPLDIKLRISGSFGEYRPASRYHHGVDIKTFEQNGIPVHAPLDGFVSRIH
                     TSEYGYGNGLFFSSGKYEFTFGHLNDFRGVRPDLEEYRLAVRILQPDHIAVVTPPPWF
                     TFKKGDQMARTGESGLGAPHLHFEVRENGTYRNPLAFSGMEIRDDTAPEILDVIAETP
                     TARFKIPAIKNGAHYDLASALPTGPVRLLIGCVDTQSALNRNGVAYLRFEVNGKEEYS
                     RTVDSIEPQQLLLADGLYHPTLTIIGKVYYYYLYARPAEYPDRTGKALVTLADVTGNT
                     ADLRIEFQKGADVTRYALMEAPQRGNAAHASAPGGLIDVTTYGQNATLGLVAAPLPSG
                     VLKEGLVQAGPSFYLSGRDLTMRDGLRLQYQGQFANASLYVVHPVSRAVNLIGAGKAA
                     GASTVFDFPMYRGEGWIVPLYDKIAPRVERVMLYGGLTPDTKEDRDPETGGWFREYYI
                     SDAGSGVRPETLQVLLNGNSYPYTWKADRSVVAVSVPRSFARDGALLSIRMEDVAGNR
                     SDWFLDTIVP"
     gene            complement(9388..10227)
                     /locus_tag="PKK76_03145"
     CDS             complement(9388..10227)
                     /locus_tag="PKK76_03145"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67490.1"
                     /translation="MIRKAFLTAALSSIFCAPLVAEDWVGSYDSSVIAETPEDPVVYS
                     LETIEKLRRSVSPRYVRVLDWRSGQGKPGLVKGILFTCEALRSKDVAVAGDFSGWKPL
                     QMKRNRNGIFFYILPIREVEEGKRILSYRYKFQSAGIWFHDPRNPARIDDGLGGYVSH
                     FELEKEDVNRQAAFRVLREAHPGKERLVEFAVHEKALGRAAKRKSVTNVAIVGDFNGW
                     NPDHDWLKRDEDGIYRLRLRLPPGEYPYVLIVDGKWVLDPYNPQTRKHSRLDDLVSFA
                     IIQ"
BASE COUNT         2564 a         2796 c         2554 g         2515 t
ORIGIN      
        1 ccagggtggc cgcagcatat ccggaggaac tagaaatcac gcgcggctct gatttgccgc
       61 cttgcgcgtc cagattcttt gcaagaggtg cctgagattt cgctgccgga gcgctggacg
      121 aggcgcgggg tgtggcggcc gcctcagaaa cagtgcgagg cttccaggct tttacttcgc
      181 ggcccggcgt cgcctcgcgt ttctgaggcg cgtcttttgg cttcttgccc gcacggggaa
      241 aatccgcccg ttcaaactcc tgatccggtt cccaggattt catttgtgcg ctccgctttt
      301 ccagaaatcg tgtcccgcat ggacgcttcg tccactgaaa aaccaacatc acctctggaa
      361 agagagattt cgcgtcgtcg cacgttcgct atcatcgctc accccgatgc agggaagacc
      421 acgctaacgg aaaaattgct gctgtacgga ggagccatcc agctggcggg tcatgtacgc
      481 gcaagaaggg acagacgcaa aacgagatcg gactggatga ccatggaaca agagcgtgga
      541 atctccatca gcacggccgc gcttcaattt gaatacaacg gttacagaat gaatctcctg
      601 gatacaccgg gccatgaaga tttttccgag gatacgtatc gcacgctgat ggcagcggac
      661 tgcgctatca tgcttctgga tgcttctcgc ggtgtagagc cacagacgat caagctcttc
      721 aaggtatgcc gcgaaagagg aattccgatc atcaccttca tcaacaagat ggaccttccc
      781 gccaaagatc cattctctct cctggacgaa gtggaaaagg tcctgggaat caccgcgatt
      841 ccgcttctat ggcccatggg ttcaggaaaa gactttcgcg gagtctacga tctggtggaa
      901 aagaaggtac atcgctacga aagagtagca ggtggcgctt cccaggttcc ggtaacggta
      961 ggcggcattg atgacccgac tctcgatgag attatggacg aatataccgc ccgcgtcttc
     1021 cgcgaaggtg cagatctggt gcagaacgca ctgccgccct tcagtcagga agatttcatc
     1081 gcaggtacaa ttactcctgt gttctttggt tctgctgtta caaacttcgg ggtgcagttg
     1141 tttctgaaca acttcctgac tcttgcccca cacccaccgc ctgtgcatct cacagacgga
     1201 acagatctac ttccatcgag cccggttttc accggattca ttttcaagct tcaggcaaac
     1261 atgaatcgcg ctcatcgcga ccgtgtcgca ttccttcgga tcacaagcgg gaaattcgaa
     1321 cgggggatga ctgcgtttgt tccgcgactc ggcaagcagg ttcaccttac aaaccccgtc
     1381 gcattcttcg ggcaagaacg ctctacgatt gacgacgcct atccaggcga catcattggg
     1441 atgatcaatc cgggcaccta tcacattggg gacattctat ccaccggtaa gccgcccgca
     1501 tttccagatc ttcccagatt cgcgccggat atgttcgcaa gcctcattcc aacggacaca
     1561 tccaaactca aacagttcaa gaaaggaatt caggaactgg cagaggaagg ggtcgttcaa
     1621 atattttcaa cacaggatgg cagtgtggtc atcggggctg cgggccagct tcagtttgaa
     1681 gtgtttcgtt ttcgcatgga agatgaatat ggtgcgccct gcagactcga ggtgatgccg
     1741 tttgaatgct ccaggtggat tcgtccttct gacgcgaaca aattcagctc ttatgacagg
     1801 attgtaaaag atgaaaaagg gcgaacagtt gtgctattta agagcgaata tcgtctaaag
     1861 agttttctac agacaaatgc tgatattccg gtttacgatc accctcctca ggcggattga
     1921 atgactaaac gccccgattc actgaaagga catttcctga tttcggaagc gaacatgtct
     1981 gatcccaatt tttttcagac agtcgttctc atggtggaac acaatgcgga aggcgcattt
     2041 ggcctcgtgg taaatagaag atcacgactc gttctatctg acatccttcc tggtttcgcg
     2101 cataaccgcg gggccagcac tcccatctac gtcggcggcc cggtgcaaca ggaatatctt
     2161 tttgtattgc attcggatct tccggacgcg gaggcaggct cagagtatcg cagcattcca
     2221 gtcaaaggag tcaatttcga acccgcattc agacgaatcg aaaactactt caacgaagaa
     2281 aactggaatg caattccagc agatgatcgc ccgggaattc atttatacct tggatattct
     2341 ggctgggcgc caggccagct ggaacaggaa atggatcgcg gaagctggat cattcaccct
     2401 gcatctgcga agatcgtctt tcacgagaat ccggaggaag gctgggccaa cgctctacgt
     2461 gacaaaggcg gcatctaccg cgtattcgca gacacaaacc cggatcctgg actcaactga
     2521 tcacgagagt tccggatcca gaaccctgtg catggccgcc atgagccccg actttgaaat
     2581 tggttttccc agatgatgtg cgaaacctgc ttcgagtgac cgcgtcacct cttctgcgat
     2641 gacgctcgcg gatactgcga tcaccggtgt atgcttctga ccaatttggt tttcgatctt
     2701 tcgcatttca gatagaattt cgtagccact catatctgga agatggagat ccatcagcac
     2761 gatatcaaat ttcttttttg agaaagactc aatggccgtg gctccgtcag gcgccactgt
     2821 gagtaaaaac tgcggaggct ttaagaatgc ttccatgagg gttctgttca cgccgtcgtc
     2881 ttctacatac aaaacagcaa cagagccttc cggaaaactc ttaataccgg tctttgactc
     2941 cactggaact acgccagtaa caggcatgaa gagaggcaaa ctcactatga atgcgctacc
     3001 ctccgacttc ttactgcgca gctcaatgac gccgtccatt tgcgcacaga gttcacgaca
     3061 aatcgcaagg ccaagacctg tcccgtccgc cgttgctgtt gtgacgtctc cctgttcaaa
     3121 cgcatcaaac acagaagtca ctttttccgc actgattcca cggccgctgt cttctactga
     3181 atagataatt ctttccaacc ctccggatgc atccgttcgc actcgcagag agattccacc
     3241 tttttcggta aacttcactg cattgtgcat tagattcacc agaatttgcc gcacgcttcc
     3301 ctcgtctcct gacacgactc gaggcaaagc tggatccact tcaatatgga ctccgaggtc
     3361 cttgctcgtc agcgcacttc gaaatagctc cgctacttga ttcagtacca gggccggctc
     3421 ataattttct cgcacgagag tgatgcgatt ttccgtacgc gccagctcga gcacctgatt
     3481 tacaatgtgc agcaaatgcc gaccggaatt cgtcatgatg cgaagatatt ctttctgctc
     3541 tggctcatga agtttttctc cgaggacttc tccgatccca agaacgccat tgagcggcgt
     3601 gcgaatctcg tgactcattc tggatagaaa gcgagcgcgt gtctgcgctg cctggcgata
     3661 tgtatcaagg agtctgtgag cgcttgtctc gcgggctacc ataacgtaga agaaaacaaa
     3721 gaaagaaacg aacatggctg tataccattc agtcacaggg acaatggggc tatctcctcg
     3781 aatgccccat acgtcagaca aaagcctgat ggtgatggct cgaacaaaag ctaggactcc
     3841 gtagaatatg ccccatttga gcggtagcat gtagacagag gccgcaaata gaacgaggac
     3901 attcagcagc ggagcaattg tcccggagga agtaacaaat tgattgagac cggttgtgat
     3961 cgatgcaagg agtgccagtg catgcgctgc tggattcagc ttccgtttca gtgcaagcac
     4021 aagtgaaatc aatgcaccca ccatgagcac gcagttgatg agagcgggca acttcaagcc
     4081 ggcattccag cgtataatac tctggattac agtgaccgga aaaacaacgg aagatagcgc
     4141 aatgacaaag cgcagctttt gcgcttcgag tttgcgcaga aactcttccg gactcgacat
     4201 atcaatgccg cctaacgccg ccgttttctt gacgatttcc ttctggacgc gccaccgcct
     4261 gaaacggtga tctctggagc tttctgcgac gcagcgggaa ggcctcccgg gcctacaccc
     4321 gggcccgaag tgctttcaaa gctacccaga gactgatgct gttcctgacc caccggctga
     4381 cgcctgctgg tctgctggat gggcccctga acttccacgc gaaacaggaa ttcagtgatt
     4441 tgttctttga tcaattgaac gagcgcatca aagagacgga atccctgaat cttgtattct
     4501 acgagaggat ttctttcaga atacgaaatg gcccagatgc cttcgcgaag gtgatccatc
     4561 gcatagaggt gatctttcca gcgcgcatca attacatcga gggcgattct tctttcaatg
     4621 tagctaaagt tttctccgcc aatcgatgct gcgtgggcat cgtagtgctc gatagatttc
     4681 tccacgatat gatcgtagag ggtttccaga ggattgcgaa cgcgtttgta gtcatccgcg
     4741 tcaagattca gatccaggcc aagcaccgtg cgcgtccatt ctttcaggct ctccagatcc
     4801 catctaaagg gatccgatcc ttcgcagaac tcaagaactt tgttctcaat gacttcgttc
     4861 atccattccc gcaaatgcgc gcgaacattc tcgttgctca tgagcttgtt gcgttccgcg
     4921 taaatgaatt cgcgctggcg attcatcaca tcatcgtatt ccagaagatg tttacgcatg
     4981 tcaaagttgt gattttccac acgcttctgt gcgcgggcaa tcgcgcgatc caccatgcct
     5041 gcttcgagct cctgcccttc ggtcatgccc agacgttcca tgatattcat cagacgctgt
     5101 ccgccaaaga ggcgcatcag ctgatcttct agactgagat agaaacgact ggatcctgga
     5161 tccccctgtc tgccagaacg accgcgcaac tggttgtcaa tcctgcgagc ttcatgtctt
     5221 tccgttccca gaatatgaag gccacccagt tcttttactt ccttgtgact tttcagccag
     5281 atttcagcgc gatctgcaat ggccttggcg agatctttgc gagcagctgt gtccagtgtg
     5341 gaaatgagat ttcgcgcttc cgcgaattct ttctttaaaa ctgcgtttct aaactttgca
     5401 gatgcttcat ctgtttcttc tagctcttcg agatctttga gatagcgcgc agcaccacca
     5461 agaacaatgt cggttccgcg acccgccatg tttgtggcca cagtgaccgc tcccggcaca
     5521 ccggcgtttt ctacaatctc agcttcgcgc tcgtggtgct ttgcgttgag gacactgtgc
     5581 gggataccag ccttcttcag aagtccagaa agcatctcgg atttttcaat cgaaattgtt
     5641 ccaaccagaa ctggttgacg cttggcatga agggccgcca gttcctgaac gattgcattg
     5701 aatttttcac gttcacttct atagacccga tccggatgat caattctctg cacgcgaacg
     5761 ttagtcggaa ttacaacgac ttccaggcga tagatcttct tgaattcctc tgattccgta
     5821 tccgcagtac cggtcatgcc tgcaagtttg ttatagatgc gaaagaagtt ctgaaaagta
     5881 atagaagcca gcgtctgagt ttcctgacga atctccacgc cttctttggc ctctatggcc
     5941 tgatgcaaac cgtctgagta ccggcggcct tccatgagac gcccagtgtg ctcatcgacg
     6001 atcatcacct cgccttcgcg aacccagtag tccacttcat gcttgtatac aagatgagca
     6061 cgcagcgcct gagtcgtatg cgccacaagc tccacgtttt ccggagcgta cagattctct
     6121 acctggagaa tctcttccat tttgtgaaca ccggcttctg tgagagtcac gttgcgcgat
     6181 ttttcgtcca catcgtagta gaaaccgaaa ataacgtgag gctcttcttt cccaggctct
     6241 acttcgcggg gcggcggttc tggagcctta tcctcttcct gaatcaggcg aatgatggcc
     6301 ttgtcgatgc tcgcgtattt gtccgtattt tcatccgtcg gacccgagat gatgaggggc
     6361 gtgcgagctt catcgatcaa aatcgagtct acttcgtcca caataccaaa gttatgtggt
     6421 ctctgaacgc gatactcagg ttcagaaacc atgttgtctc tcaagtagtc aaagccgtat
     6481 tcgttgttcg ttccataagt aatgtcgctg ttataggcct gctttctttc ttccgtagaa
     6541 aggttgtgct gaatgactcc cacgctcaca cctaacagat cgtagatggg cttcatccag
     6601 gtggcgtcgc gtctggccag gtagtcattg actgttacaa tgtgaactcc cttacctgac
     6661 aatgagttga gataaatagc gctggttgca gtgagagtct taccttctcc cgttttcatt
     6721 tcagcgattt tgccctgatg aagaaccatg ccaccaatca actgcacatc aaagtgacgc
     6781 aggcccagag ttcgaacgga tgcttcgcgc acacatgcga atgcttcagg gaggatgtcg
     6841 tcgagtgttt cgcctctggc aagtcgtgct ttgaattcag ccgtctttgc accgagctct
     6901 tcattgctca aagccttcag tgagggttca agtgaattga ttttttcaac gatgggcagg
     6961 agtttgcgta tatcgcgctc atgctttgag ccgaagatga gcgtcagagc cttctgaaac
     7021 ataatctgtc aaattccccg gcggacacaa ggaatccacc gtttttcagg cggataagag
     7081 caagtctctt cgctcgaaga atgaatgaac agcatagact acaaagtaag cacccaagat
     7141 atcaatcgtg tagtgctcat gcgttaaaat gagtaaagca actgtgacta tatgaaagaa
     7201 cacagatacc gtcctgatcc acccccgggt tacgagacaa aaaaggaatg tggttgcggt
     7261 atgccctgag aagaaaagat cctgggtcaa ataggcggca gacaaaccac ttcctccgta
     7321 cagaacatcg agaggaatcc actgccgtat caggagggga agagtgatcg attcccgggt
     7381 caaagaaagc atggcgggtg gcgcatctga catcacagcg agaggtggac caagactggt
     7441 tgcgaaaatg aagagtccgc gaaatacagt gactactgcg cccacgcgca tatagttcac
     7501 gcaggccatg ggactgcgaa tcgccagaaa cgcaatgaag accagtactc caggtagcca
     7561 gatggttgaa ttgtatacag caagagtgcg atcggcgtgt atccactcgt gaagtcgatc
     7621 cggcaaagtg ggtccgcgcg tagtctccgc gagaagacaa aacgtctgca tgatcacgta
     7681 agaagaatac tgatagaaaa ttccgagtgc gatctctcgt cgtgggtaac tcatgggaca
     7741 atcgtgtcca aaaaccaatc ggaccgattg cctgcaacat cttccatgcg aatgctcaga
     7801 agcgcgccat cgcgcgcgaa ggacctggga acgctgaccg ccaccacact gcgatccgcc
     7861 ttccacgtat acgggtacga atttccgttc agcaagactt gcaaagtttc cggacgaact
     7921 ccgctgcctg cgtcagatat atagtattcg cgaaaccatc ctccggtttc tggatcacga
     7981 tcctcttttg tgtcaggcgt tagaccaccg taaagcatca cacgttccac tcgaggcgca
     8041 attttgtcat aaagcggaac aatccaacct tctccgcggt acatgggaaa atcaaataca
     8101 gtcgaagcac ccgcagcctt ccccgcgcca atcagattga ctgctctgga aacaggatgc
     8161 accacataga gagaagcatt cgcaaactgc ccctggtact gcaacctgag accatcgcgc
     8221 atggttagat ctctaccgct caaatagaaa gatgggccag cctgcacaag accctccttt
     8281 aagactccag acggcaatgg tgcggccacc agacccagcg tggcgttctg gccgtacgtc
     8341 gtcacatcga tcaaaccgcc aggtgccgat gcatgcgccg catttcctct ctgcggagct
     8401 tccatcaacg cataacgggt aacatctgcc cccttttgga attcaattcg aagatccgca
     8461 gtgtttcctg ttacatccgc cagagtcaca agtgcttttc ccgtacgatc tggatattcc
     8521 gccggtcttg cataaagata gtaatagtaa acttttccga tgatcgtaag agtcgggtga
     8581 tagagaccat ctgccaggag tagctgctgc ggctctatgg agtccacggt tctagagtat
     8641 tcttctttgc cgttcacttc aaaacgtaaa taggcaactc cgtttcggtt gagcgcagac
     8701 tgcgtatcaa cgcatccaat caaaagacgc actggcccgg tcggaagagc ggacgcaaga
     8761 tcatagtgtg caccgttctt gatggctgga attttaaaac gagcagtggg agtctccgca
     8821 atcacgtcga gaatctcagg agccgtatca tcccgaatct ccatacccga gaaggcgaga
     8881 ggatttctgt acgtaccgtt ttcgcgcact tcaaaatgaa ggtgaggcgc gccgagcccg
     8941 ctttcgccag tgcgagccat ttggtctcct tttttgaatg tgaaccaggg aggtggtgta
     9001 accactgcta tgtgatctgg ctgtagaatt cgcacggcca gcctgtattc ttcaaggtcc
     9061 gggcgaacgc ctctaaaatc gtttagatgg ccaaacgtaa attcgtattt tccactcgaa
     9121 aagaacaatc catttccata tccgtattca gatgtgtgaa ttcttgaaac aaagccgtct
     9181 aacggtgcat gcactggaat cccattctgc tcaaatgtct tgatgtccac accatgatga
     9241 taccgactcg caggacggta ctctccaaaa cttcctgaaa tccgaagctt gatatccaga
     9301 gggggctcga gtacgggcgt atgaggatcg gcaccaatca cccagaagga tgccagtgca
     9361 aacagagcca gcagtgcgaa tctgaatcta ttgaatgatt gcaaatgaaa ccagatcatc
     9421 gagacgcgaa tgtttgcgtg tctgcgggtt gtaaggatca agcacccatt tgccgtccac
     9481 aatcagaacg taaggatatt cgccaggagg gagcctcaga cgcagcctgt agattccgtc
     9541 ttcgtctctc ttcagccagt catgatccgg gttccaaccg ttaaaatcgc caacgatcgc
     9601 cacgttcgtc acagactttc tcttcgcggc ccgacccagc gctttctcat gaactgcaaa
     9661 ctcaaccagc ctctcctttc cgggatgcgc ttcgcgcaga actcggaagg ccgcctgacg
     9721 atttacgtcc tctttctcca gctcgaaatg ggagacgtag ccgcccaggc catcgtcgat
     9781 tcgcgctgga tttcgcggat catgaaacca gattcctgca gactggaact tgtatcgata
     9841 cgataggatt ctctttccct cttctacttc gcgaattggc agaatataga agaaaatgcc
     9901 attccgattg cgtttcatct gcaatggctt ccagccactg aagtctcctg ccacggccac
     9961 atccttcgaa cgaagggctt cgcatgtaaa aagaatcccc ttgacgaggc ccggtttgcc
    10021 ctggcccgaa cgccaatcga gcacacgaac gtatctggga ctcacggatc tacgcagttt
    10081 ttcaatggtt tcgagagaat agactacagg gtcttcgggc gtctctgcaa tgacggacga
    10141 atcgtaggat ccgacccagt cctcggccac cagaggcgcg caaaaaatgg aggacagggc
    10201 agctgtaaga aatgctttgc ggatcatctc cctaaaagtg tcgataatca aataggtttc
    10261 cctgagtagt ttcctttaag cagagcaccg tggccgaaga aaagatcgaa cttaccccgg
    10321 atgagttaaa gcaggttggc acactgctcg aaccgcttac gcaggcgggc agacctctct
    10381 ttcccatgga agagaagttc aagtctgaca tgacggacct cgaccgcat
//
LOCUS       DAORFF010000045        10407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2928_length_10407_cov_4.001352, whole genome shotgun sequence.
ACCESSION   DAORFF010000045 DAORFF010000000
VERSION     DAORFF010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10407
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2928_length_10407_cov_4.001352"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PKK76_03150"
     CDS             <1..802
                     /locus_tag="PKK76_03150"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL67491.1"
                     /translation="QEKHRSFMRDVGHAREIQSGLITTEFPSMHRIKIVGKYMPMQEL
                     GGDFYNVRRLDEHRLSIFISDVTGHGIASAFITAMIKISLDSLPMNVLIHPDKVLNHL
                     NEALLDKIMDRFITGIYGILDEDTMEFHFCSAGHHPPALHFKAASGQVEELMARGRLL
                     GAFDSLELVTESIPLKSGDRILLATDGLYEPFDDKGEMFGLARLKEIFHRELTHSLDP
                     LFLAIHRYTKGARQADDMAGLLILVDPVPTTRPVPSAHSQSADSSSTR"
     gene            complement(720..2054)
                     /gene="argG"
                     /locus_tag="PKK76_03155"
     CDS             complement(720..2054)
                     /gene="argG"
                     /locus_tag="PKK76_03155"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNL67492.1"
                     /translation="MSRIYRELPPKGTRIGIAFSGGLDTRAAVAWLAEQGMEVCAYTA
                     DLAQPDETDIASIPQIALDHGAKSARMVDCRDALAAEGLTAIQCGAFHIHTGGKKYFN
                     TTPLGRAVTGTHIVKAMREDDVHVFGDGSTHRGNDIQRFYRYGILVNPDLRIYKPWLD
                     QAFVNAFGGRKEMSDYLQKIGKPYRASAEKAYSTDCNMLGATHEAKDLEFLDRGMEIV
                     EPIMGKAFWKSDVSIEPEMVRVDFEHGVPVAINNTRKSGHDLILLANSIAGKHGLGMS
                     DQIENRVIEAKSRGIYEAPGMALLHICYERLLTSIHNENSLDQYYNHGRRLGRLLYEG
                     RWYDPEAMMMRESFRWISYPVTGSVVIELRRGDDYTIRKTESANATYHPEKLSMEKTE
                     SAFTPSDRIGQLEVQNLNISDNRQLLTEYLKNPQIANAQKELDEWLEQDRRG"
     gene            complement(2099..4180)
                     /locus_tag="PKK76_03160"
     CDS             complement(2099..4180)
                     /locus_tag="PKK76_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNL67493.1"
                     /translation="MRRLSLILLAAAFWDCKTIQQPAKPETDNKPRPITTEAPVTSEI
                     DRWVPEPAELLSEKPETDDASLAIVRQRMRDFVVRNSINANDDLEFSSYSDHTLEPKE
                     LEFLEKTATSDPKPGRQLLAVEILAQSRLLKGDFAGAAGVWKTHRIPGYERHTQALIK
                     ILEGQTEVIELQNFGSPVNSDQTEYDAVPDLSDKRIYFTRYDHEKKMSDPPGEDIWIA
                     EKKSDGSWGARPLSELNTPSHDSVVSASADGSSLYLFGHYPGSLGSGDIFKSTLTAKG
                     WSKPVPLPPPINSAFFDSDAVETPNGKAIIFASDRPGGYYPYHPKQQEFYGGGRAGNV
                     DLYISFVKPDGSYTLPRNLGPVINTPGSDRNPYLHADGKTLYFSSDGHPGLGGHDVFR
                     SVRLDDTWQKWTEPENIGRYVNGPGNDTGFRLNAHGTQGLISGSHKDTHGPSDIYRIM
                     PIPVRAKPAEEVSLLRGYVKDQKGQPVEADVAWKKKGEKESEGKAKSRADTGEYVLPL
                     AQNKTYDIQVSKPGFFEKKFEVQTPPPEKAAKTPDLDIPIVIKKDPAYKPTERDMAKS
                     DQKAEPVEPRVIVRPSPAKPPLARVHFSIGGFQIQNPDALTPTLTYLKQNPKSRIMIE
                     AHADSTGNAAFNRQLSERRARAVYQYFVSRGISPTRVAYRGIGARKPAATNRTGVGRK
                     INRRAIVRILP"
     gene            4273..4587
                     /locus_tag="PKK76_03165"
     CDS             4273..4587
                     /locus_tag="PKK76_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67494.1"
                     /translation="MSLFSSGQEIQLTLRGEQIQGLILFEKAKEIALRLEDHPVFKND
                     SIWTLLKNEFIHGEFNEGPFRCSFDTRIIDLGEDRASTRTDWILSLQIPTRIRRVNQK
                     LQ"
     gene            4584..5162
                     /locus_tag="PKK76_03170"
     CDS             4584..5162
                     /locus_tag="PKK76_03170"
                     /inference="COORDINATES: protein motif:HMM:NF025314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HNL67495.1"
                     /translation="MRIVPRNLPLVSVEEIQSEIETMAHASTLAERQGSAPAAATSAN
                     EIPASNAVQSPNNHYAESEIPPGITPGIADVRALMKLDSVQFLHGAYHSVLAREMDAD
                     GEDVYKTFLWNGMHRGEIILRIRFSREGRRIHAKLHNGFLTWVDLLLWRLPLLGYFYR
                     IVRELVRLPGTMRRLEMNQAELRRKMDRNGRG"
     gene            5159..6235
                     /locus_tag="PKK76_03175"
     CDS             5159..6235
                     /locus_tag="PKK76_03175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNL67496.1"
                     /translation="MKRAAILVPRLDPGDAVGNDARSQAELLNSRGWEARIFASAWSR
                     QTPARPVSGLKRFIRNHEDLLIHHPASLWKEGDDAFQASPARRIIKYHNVTPASFFEA
                     HNNDYANICRKTREALRPLVSSAHLVLCDSTFNLEDIVLEGATRNQCKVVPPFHRLES
                     RDWLDLDPLWVERFTRPGHPMFLSVGRIVPNKALEKCIEALGVMQSRYGIPAKLVLAG
                     RIDRRLGSYIRFLEKKSVEHCIPGTVIFADHVSDTRLRTLFLCAVAYLVTSEHEGFCV
                     PVIEAMKSRLPVIACGKTAVAETLGGQGIYTETFDAHLFASAMERLVKSKESCAIIAD
                     HASHAYETRFHSEELFRIFLEALS"
     gene            6232..7380
                     /locus_tag="PKK76_03180"
     CDS             6232..7380
                     /locus_tag="PKK76_03180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNL67497.1"
                     /translation="MNKKVALVIQRYAPDTAGSEQHARITASLLAEHYEVEVLTTTAS
                     DMVTWRNHFSPGETAEGNIKIKRFEVTSGRQKTWEGLYALMLRRLKTHGETGWPLSLE
                     EEWIRLQGPHSEGLLAYLSEHQHNYDAVIFYTYLYSPTYFGTACVEKNRSLLMPTLHD
                     EPAARLACYAHAARRVKRIVWNTFAEQKLGEKLWGNLPGTMTGLPIDIPDPVSPVRAA
                     PYVLYSGRIEPGKGCDDLVAMIQNSSARVDLRLTGTKYMKLSGSRIHYEGYVTAQKKL
                     ELLAGSICFAMPSPNESLSIATLEAMAAGVPVLVNGKNPVLREHVEESGGGFCYESSG
                     EFDEAILTLKDNADLRSTMGQKGRAYVQRRFSHRSIRDAWISAIESGA"
     gene            complement(7383..8909)
                     /locus_tag="PKK76_03185"
     CDS             complement(7383..8909)
                     /locus_tag="PKK76_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67498.1"
                     /translation="MRFWISCALFVWACACLEGTLSHNLYSSRSPELSGLHHYFNADA
                     MYLPALFQDWQAGKDLHGWTLPPAPYLFPDLPIYFTFLKFFPFKSAAFLAGIFLLLFN
                     LFSVLYFAKRGLRVKRSNSSVLLTGAILLCVLARCAIAEEFYSLIVPTFHAGVLGIAF
                     LTFGWMLQSEHSRSRLPHLVATMTLASLSDALIIALVLPAAVSWILVRRTRSSVLAAL
                     AIATGLILGRLAVGPMSDYLAPIFRVSPERDVLHFLTSEHALSTIVNDLSQPVYLLPV
                     AFAVVMIAYAMSVKQRQGLILPAVAILSAGVAASAFSHQVGLPRMEPRYALMPLFAAG
                     TLAGMWLFRSRLFFVLAAILVSVTIFKTRTEAGIHPIEEAANCLSGLSETHGIKKGFA
                     DYWHSKQYSVLTDRRVHIEQIHVNAGARIVEPRMWINNLDGYFPENRPEDFDFLIAND
                     LPRDVLLERFGEPVLKRSCGHLDVWIYRKDNRGVVPFTFDSFQLWRNAIGRPARIKDK
                     "
     gene            complement(8909..9388)
                     /gene="sixA"
                     /locus_tag="PKK76_03190"
     CDS             complement(8909..9388)
                     /gene="sixA"
                     /locus_tag="PKK76_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNL67499.1"
                     /translation="MKLLLARHAEAESSTPDSERSLTEKGKSDARRMARVLRQTGWQF
                     EEILCSPLRRTVQTAELYGAGLGTSAKNAVQAVPFLAPTADRTRAIEHLGEMGIRGAA
                     LWVFHMPDVAEVASWLLGIPAASLYVTPGTIIALNLAFPVPGKAMLVWSMQPEHLPE"
     gene            9473..10180
                     /locus_tag="PKK76_03195"
     CDS             9473..10180
                     /locus_tag="PKK76_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNL67500.1"
                     /translation="MGPSLQEINDNIAIQVSNGQYLTLKTHRMTEMVEDHIKHAIESI
                     LAKTNKSNLIHVIYTILKELVINGCKANQKRVFFEERGYNISDSQQYAVGNEEYKKIF
                     SEEMALEYGIKAKAKGLYCLIDFLFDENGLTIEVSNNTTITPQEEASMREKLKKAMGY
                     NDIAEFYLDQAMSGGETEGAGLGLALIIILLKGENVDPKYFRIMIDKERTVARMEIPF
                     TDRFVSKRDLGSKVVQG"
     gene            10180..>10407
                     /locus_tag="PKK76_03200"
     CDS             10180..>10407
                     /locus_tag="PKK76_03200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNL67501.1"
                     /translation="MPRAGNSLLSVCIIALNEELRLPRCLESLSFADEIVVLDGGSVD
                     GTIKLAKSYPRVRVEKRRFDDFVRQKNHVISL"
BASE COUNT         2508 a         2571 c         2719 g         2609 t
ORIGIN      
        1 tcaggagaaa catcgttcat tcatgcgcga tgtgggtcat gcgcgcgaga ttcaaagtgg
       61 attgattaca actgaatttc cttccatgca tcgcatcaag attgttggaa aatacatgcc
      121 catgcaggag ctcggaggag atttttataa cgttaggcga ctggacgagc ataggctgag
      181 catcttcatc agcgacgtaa cgggacacgg cattgcaagc gcattcatca ctgcaatgat
      241 caagatttcc ctcgatagtc tcccaatgaa tgtactgatt cacccggaca aggtcttgaa
      301 tcatctaaac gaagcgttgc tcgataagat catggatcga ttcatcacgg gaatctacgg
      361 aatcctggac gaagacacga tggaatttca cttttgctct gccggacacc atcctcctgc
      421 tttgcatttc aaggccgcct cggggcaggt ggaggaactg atggccagag ggcgattgct
      481 gggtgcgttc gattcactgg agcttgtgac tgaatcgatt ccactgaaat caggggatcg
      541 gattttgctc gcgacagatg ggctctacga accatttgat gacaaagggg aaatgtttgg
      601 cctcgcgcga ttaaaggaaa tctttcatcg cgaactaact cattctctgg atccactttt
      661 tctggcgatc catcgttata cgaagggcgc caggcaggca gacgacatgg ccggtcttct
      721 tatcctcgtc gatcctgttc caaccactcg tccagttcct tctgcgcatt cgcaatctgc
      781 ggattcttca agtactcggt gagcaattgt ctgttgtcag agatgttcag gttttgaact
      841 tccagttgtc cgattcgatc tgaaggagtg aacgcacttt ccgttttctc catagaaagt
      901 ttttccggat ggtaggttgc attcgcggat tccgtcttgc gaatcgtata atcatctccg
      961 cgacgaagtt caatgactac ggacccggtc acaggataag atatccagcg aaaggattcg
     1021 cgcatcatca tagcttccgg atcataccag cgtccttcat aaagaagtct gcccaggcgg
     1081 cggccgtgat tgtagtactg gtccagtgag ttttcattgt gaatggaagt gagtagtctt
     1141 tcgtagcaaa tgtgcagaag tgccattcct ggcgcttcat agatccctct gctttttgct
     1201 tcaatgacac ggttttctat ctggtccgac atcccaagtc cgtgcttgcc tgcgattgag
     1261 tttgcaagaa ggatgagatc atgtccgctc tttcgcgtgt tgttgatagc gacgggcaca
     1321 ccgtgttcga aatcgactcg caccatctct ggttcaatgg atacgtccga cttccagaac
     1381 gctttgccca taatgggctc tacaatttcc atccctcggt ccaggaattc caggtctttt
     1441 gcttcgtgag tggcgccgag catgttgcaa tccgtggagt atgctttctc cgcactcgca
     1501 cggtatggtt ttccaatttt ttgcaagtag tccgacattt cttttctacc gccgaacgca
     1561 ttcacgaatg cctggtccag ccagggtttg tagattcgca ggtcgggatt taccaggatg
     1621 ccatatctgt agaatctctg aatgtcattg ccgcggtgag tacttccatc gccgaacaca
     1681 tgcacgtcat cttcgcgcat tgctttgacg atgtgtgtgc ccgttacagc ccttcccagt
     1741 ggcgtcgtat tgaagtattt ttttccgccg gtgtgaatat gaaaagcacc gcactgaatc
     1801 gcagtgagac cttctgcagc cagtgcatcg cggcagtcca ccatgcgcgc actctttgct
     1861 ccgtgatcca gagcgatctg cggaatagaa gcgatgtctg tttcatcggg ctgcgcaaga
     1921 tctgcggtgt acgcacagac ttccattccc tgttctgcca gccacgcaac ggcggccctg
     1981 gtatcaaggc cgcccgagaa agcgattccg attcgtgtac cttttggcgg aagttctctg
     2041 taaatgcggc tcatgagcca ttattttcat ttgttcaaag aactcaaatt ctttcgcatc
     2101 acggaagaat tcggactatt gcccggcggt tgatctttct tcccacaccg gtccggtttg
     2161 tggcggcagg tttccttgct ccgattcctc tgtaggcaac cctggtgggc gagataccgc
     2221 gagagacgaa atattgatac actgcgcgcg cccggcgctc gctcagttgt ctgttgaacg
     2281 ctgcgtttcc agtgctatcg gcgtgagctt cgatcatgat tctcgatttt gggttttgtt
     2341 tcagataggt gagggtcgga gtcagcgcgt cggggttttg aatctgaaat cctccaattg
     2401 aaaagtgaac tcgcgccagt ggaggtttcg cgggagaagg gcgaacgata acccggggtt
     2461 cgacaggctc ggccttctga tctgattttg ccatgtctct ttcggttggt ttgtaggcgg
     2521 ggtccttttt gatcacaatg ggaatgtcta gatccggcgt tttggctgct ttctccgggg
     2581 gcggagtttg cacttcgaac ttcttttcga agaatcctgg ctttgaaacc tggatatcgt
     2641 aggttttgtt ctgagcgaga ggcaatacat attcgcctgt gtctgcgcgg ctcttcgcct
     2701 tgccttcgct ttctttttcg cctttcttct tccaggcaac gtccgcttcc accggttgtc
     2761 ctttctgatc ttttacatag ccacgcagta aactgacttc ctctgcgggt ttcgcgcgaa
     2821 caggtatggg cataatgcga tagatatcgg acggtccgtg agtatctttg tggcttccag
     2881 aaatgagacc ttgcgttccg tgcgcattga gtctaaaccc tgtgtcgttg cctggcccgt
     2941 ttacgtatct gccaatgttt tctggttcag tccatttctg ccatgtatcg tccaatcgca
     3001 cgctgcgaaa tacatcgtgg ccgccgagcc ccggatgtcc atcggatgag aagtagagag
     3061 tttttccatc ggcgtggaga tacggattcc tatctgatcc tggagtgttg attacgggcc
     3121 caaggtttcg cggtagagta taagatccat ctggctttac gaagctaatg taaagatcca
     3181 cattgcctgc gcgtccgccc ccatagaact cctgttgttt gggatgatac ggataatacc
     3241 cgccagggcg atcgcttgca aagatgatgg cttttccatt gggagtctcc actgcatcgg
     3301 aatcaaagaa agcgctgttg atcggaggcg gaagcggcac aggcttgctc catcctttcg
     3361 cagtgagagt ggatttgaaa atgtcccccg atccgagcga tccgggatag tgaccaaata
     3421 aatacaaact gcttccatct gcggacgcgc tcaccactga atcgtggctc ggagtgttca
     3481 gttctgagag tggtcgcgcg ccccaggagc cgtccgattt tttttccgcg atccagatgt
     3541 cctctcccgg cgggtcagac attttctttt cgtgatcgta tcttgtgaaa taaattctct
     3601 tatcagacaa atccggcacc gcatcgtact cagtttgatc cgaattcact ggagaaccaa
     3661 agttctgcag ttcaatgact tccgtctggc cctcaagaat ctttatgagc gcctgagtgt
     3721 gtctttcgta tcccggaatg cgatgagtct tccatactcc ggctgctcct gcaaaatcgc
     3781 ctttgagcag tcgggactga gcaagaattt ccacggcaag aagctggcgt ccgggttttg
     3841 gatcagacgt ggcggttttt tctagaaact ccaactcttt tggctccaga gtgtgatcag
     3901 aatagctgga aaactcaaga tcatcgtttg cgttgataga attgcgaact acgaaatccc
     3961 gcattctctg acgaacgatt gcgagcgaag catcatctgt ttctggcttt tccgaaagca
     4021 actccgctgg ctctggaacc cagcgatcga tttccgaggt tacaggcgct tctgttgtga
     4081 ttggtctggg cttgttgtct gtttcgggct tcgcgggctg ctgaatggtt ttgcagtccc
     4141 aaaaagcggc ggccaggagg atcaaggaca atcggcgcat agcgcatgaa ttgcatttcc
     4201 tccaaggagg gtccagcttt ttgcaaacgg cgaatgactt gacctccata tccggccgga
     4261 aggatttcat ttgtgtctct attctccagc ggtcaggaaa tccagcttac actacgcggt
     4321 gagcagattc agggtctgat tctttttgaa aaggcaaagg agattgcgct gcgcctggaa
     4381 gatcatcctg tctttaagaa cgattccatc tggacattgc tcaagaacga attcattcac
     4441 ggagaattca atgaaggtcc attccgctgt tcgtttgata cgcgcatcat tgatctcggt
     4501 gaagacagag catccacaag aactgactgg attttgagtc tgcagattcc tacacgcatt
     4561 cggcgcgtga atcaaaaatt gcaatgagga tcgtaccaag aaaccttcct cttgtttccg
     4621 ttgaggagat tcagtcagag attgagacca tggctcacgc gtcgactctg gccgagcgcc
     4681 agggatcggc cccggccgcg gctacttctg caaacgaaat tccagcatcc aatgccgttc
     4741 aatcgccaaa caaccattat gcggagtctg aaattccacc aggaataact cccggaattg
     4801 cagatgtccg ggctcttatg aaactggaca gcgttcagtt tctgcacggc gcgtatcatt
     4861 ccgttctggc gcgcgaaatg gatgcagatg gagaagacgt ctacaaaact tttctctgga
     4921 atgggatgca tcggggagaa atcattttac ggattcgttt ttcgcgcgaa ggcagacgga
     4981 tccatgcgaa attgcacaat ggtttcttga cctgggtcga tttgctactc tggcgccttc
     5041 ctttgctggg ttatttctat agaattgtgc gcgaattggt acgattgccc gggactatgc
     5101 gcaggctgga gatgaatcag gcggaattgc gcagaaaaat ggatcggaac ggacgcggat
     5161 gaaacgggcc gcgattctgg ttccaagact ggacccgggc gacgctgttg gaaatgatgc
     5221 gcgatcgcag gctgaactgc tcaactcacg aggatgggaa gccaggatat ttgcgtccgc
     5281 ctggtccagg caaacgcccg cgcgacctgt ttccggcctg aaaagattca tcaggaatca
     5341 cgaagatcta ctcattcatc atcctgcttc gctctggaaa gaaggagatg atgcgtttca
     5401 ggcaagtcct gcgaggcgca ttatcaaata tcacaatgta accccggcat cctttttcga
     5461 agcacataac aacgattatg cgaacatatg caggaagaca cgagaagctc ttcgtcctct
     5521 agtctcgtcg gcgcacctgg tgctttgtga ttctactttc aacctggaag atatcgtgct
     5581 cgagggagct acgcgtaacc aatgtaaggt ggtcccgccg tttcacagac tcgaatccag
     5641 ggattggctg gacctggatc cgctctgggt ggaacgattc acccggcctg gccatcccat
     5701 gtttttgagt gttgggcgca ttgttcccaa caaggctctt gagaaatgca tcgaagcgct
     5761 gggtgtcatg cagagtagat acggaattcc tgcgaagctg gttctggccg gaagaatcga
     5821 tcgcaggctg ggctcttaca tacgctttct tgaaaaaaag tctgtagaac attgcatccc
     5881 cggaacagtg atctttgcag accacgtttc agacactcgt cttcgcacct tgtttttatg
     5941 cgcggttgcg tatctggtca caagcgagca cgaaggattt tgcgtgccag tgattgaagc
     6001 gatgaagtcg cgtctgcctg tgattgcgtg cggaaagacc gccgttgccg agactctggg
     6061 aggtcaggga atctatactg agacgtttga tgctcacctg tttgcatccg ccatggaaag
     6121 attggtgaaa agcaaagaga gttgcgccat catcgccgat catgcatcgc atgcgtatga
     6181 gactcgcttt cactctgaag agcttttccg cattttttta gaggcacttt catgaataag
     6241 aaggtggccc ttgtgatcca gaggtacgct ccggatacag ctggatccga acagcacgct
     6301 cgcattacag ctagtcttct tgcggagcac tacgaggtag aagtgttgac cacgactgcc
     6361 tcggacatgg tgacctggag gaatcatttc tctccggggg agacagccga gggtaatatc
     6421 aagatcaaac gctttgaggt cacgagtggt cggcaaaaga cctgggaagg actctacgcg
     6481 ttgatgctgc gccgtttgaa gacgcacgga gaaactggat ggcctctctc tctagaagaa
     6541 gaatggattc gtcttcaggg accacatagc gaaggactcc tcgcatactt gagcgaacat
     6601 caacataact acgatgccgt tatcttctac acatatctat attcacccac atacttcggg
     6661 actgcctgcg tagaaaagaa cagatctctt cttatgccca ctctacatga tgagccagcg
     6721 gcaaggcttg cctgctatgc gcatgccgct cgtcgcgtga agcgcattgt ctggaacacc
     6781 ttcgcagaac aaaagttagg cgagaaactc tggggaaatt taccgggaac catgacaggt
     6841 cttccaatcg acattccgga tcccgttagc ccggttcgtg ctgcgcccta tgtcttgtac
     6901 tcaggtcgca ttgagcccgg gaaggggtgt gacgatctcg ttgcaatgat tcagaattct
     6961 tctgcgcgcg tggatcttcg actcaccggg actaagtaca tgaaactctc cggctctcgc
     7021 atccactatg aagggtacgt cactgctcag aagaaactag aactcctggc cggaagcatt
     7081 tgttttgcta tgccatcccc gaatgaaagc ctgagcattg ccactctcga agcgatggcg
     7141 gccggtgtgc ctgttcttgt gaacggcaaa aatccggtgt tgcgcgaaca tgtagaggaa
     7201 agcggcggtg gattttgcta tgaatcgtca ggagaatttg acgaagcgat tcttacgctc
     7261 aaagacaacg ctgatcttcg gtccaccatg ggacagaagg gccgtgccta tgtgcaaagg
     7321 cgattcagcc atcgctcaat tcgcgacgcg tggatttccg cgatcgaaag cggagcctga
     7381 tcctatttgt ccttgattct ggcgggtctt ccgattgcat ttctccagag ttgaaaagaa
     7441 tcaaaggtga agggaactac tccgcggttg tcttttctat agatccagac atccaggtgg
     7501 ccacaggaac gtttgagaac tggttcacca aatcgttcga gcaatacatc tcgcggtaga
     7561 tcattcgcga tcagaaaatc aaaatcctcc ggcctgttct cagggaaata tccgtccagg
     7621 ttgttgatcc acatgcgagg ttctacaatg cgcgctcctg catttacatg gatctgttcg
     7681 atatggacgc gtctgtctgt cagaaccgaa tactgtttgg aatgccagta gtctgcgaat
     7741 cctttcttga tgccatgagt ttcggataat ccactcaaac aattcgccgc ctcttcaatc
     7801 ggatggatcc ctgcttctgt gcgagtcttg aaaatggtaa cagagacaag gatcgctgcc
     7861 agaacaaaaa atagtctcga tcgaaagagc cacataccag ccagagttcc ggccgcgaac
     7921 aaaggcatga gcgcataacg tggctccatg cgcggaagtc caacctggtg actgaaagcg
     7981 gatgcggcca cacctgctga aaggatggcg acagcgggga ggatgagccc ctgcctttgt
     8041 tttaccgaca tggcgtacgc gatcatgacg actgcaaatg caacgggaag taaatacact
     8101 ggctgcgaga gatcattcac gatcgtggat agcgcgtgct ccgaagtaag gaagtgaaga
     8161 acatcccttt ccggtgagac gcgaaagatc ggtgcgaggt agtctgacat ggggcccact
     8221 gcgaggcgac ccagaatcaa tcctgtggca atggccaggg ccgcgagaac agaagatcgg
     8281 gttcttcgca ctaaaatcca ggaaaccgcc gcaggaagaa ccagggcaat gatgagagca
     8341 tctgaaagag aagcgagagt cattgtcgcc acaagatgtg gtagtctgga tctggaatgt
     8401 tcagattgaa gcatccaacc gaatgttaga aatgcaattc ccaggacccc tgcgtgaaat
     8461 gtgggaacaa tcaacgaata gaattcctcc gcgatggcgc agcgcgcgag gacacacaat
     8521 agaatggcac ccgtgagtag cacagatgaa ttggatcgct taactcgaag gccccttttt
     8581 gcaaagtaca gaacactaaa taaattaaac aatagaagaa agatgcctgc caggaacgcg
     8641 gcggacttaa acgggaaaaa cttaagaaat gtaaagtaaa tcggaaggtc cggaaataaa
     8701 tacggtgcgg gcggcagagt ccatccgtgt agatctttac ctgcctgcca gtcctgaaag
     8761 agtgcgggta gatacattgc atctgcattg aaatagtgat gaagtccgga caattcgggc
     8821 gaacggctag agtataaatt gtgcgaaaga gttccctcca ggcaagcaca ggcccagaca
     8881 aacaatgcgc atgagatcca gaagcgcatc actctggaag atgctccggt tgcatggacc
     8941 agacgagcat tgctttgcct ggaacgggga atgcaaggtt gagcgcgatg attgtacccg
     9001 gcgtaacata gagcgatgct gccggaattc ctaaaagcca tgacgccact tccgcaacat
     9061 ccggcatatg aaatacccag agagctgctc ctcgaatccc catctcgcct agatgttcaa
     9121 tcgcacgtgt gcggtccgct gttggcgcga gaaaaggtac ggcctgcacg gcattcttcg
     9181 cactcgttcc aagacccgct ccataaagct ctgcagtttg aacggttctg cgtagcgggc
     9241 tgcatagaat ttcctcgaac tgccaaccgg tctgccgcag tactcgagcc attcggcgcg
     9301 cgtcgctttt tcccttttca gtcaggcttc tttctgaatc tggcgtggac gactctgcct
     9361 ccgcgtgccg ggccaggagt agcttcataa tgtacgcgaa attgtccgta ccggggccgt
     9421 aaagcagaaa gcatgccttg acaaaccagg tttttgggtc gagtgtgtct gcgtgggtcc
     9481 gtctctacag gaaattaatg ataatatagc aattcaggtg tccaacgggc agtatctgac
     9541 cctcaagaca catcgaatga ctgaaatggt agaagaccat atcaagcatg cgattgaaag
     9601 catcctcgcg aagacaaaca aaagcaatct gatccacgtg atttatacga tcctcaaaga
     9661 gctcgtgatc aacggatgca aagcaaatca aaagagagtt ttttttgaag agcgtggtta
     9721 caacatctcg gattcgcagc aatacgcagt tggaaatgaa gagtacaaga agatcttcag
     9781 cgaagagatg gcgctcgagt atggaatcaa agctaaggcc aaagggctct attgcctcat
     9841 cgattttctt tttgatgaaa atggtctgac cattgaagta agcaacaaca caaccatcac
     9901 accgcaagaa gaagcgagca tgcgcgagaa gctgaaaaag gcaatgggtt acaatgacat
     9961 cgctgaattt tatctcgatc aggcaatgtc gggcggagaa acggaaggtg ctggacttgg
    10021 tctggcgttg atcatcattc ttttgaaggg tgagaacgta gatccaaaat actttcgcat
    10081 catgatcgac aaagagcgaa ccgtggcacg gatggaaatt ccattcacgg atcgctttgt
    10141 aagcaagaga gacctgggct ccaaggtagt tcagggttga tgccccgcgc gggcaattca
    10201 ctcctctccg tctgcatcat agccctcaac gaggaactgc gtcttccacg ctgcctggaa
    10261 tctctttcgt ttgcagatga aattgtcgtc ctggacggcg gatctgtgga tggaaccatc
    10321 aagctcgcca aatcctaccc acgagtccga gtggaaaagc gcagattcga cgactttgtg
    10381 cgtcagaaga atcatgtcat ttcgctc
//
LOCUS       DAORFF010000046        10404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2932_length_10404_cov_3.191999, whole genome shotgun sequence.
ACCESSION   DAORFF010000046 DAORFF010000000
VERSION     DAORFF010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2932_length_10404_cov_3.191999"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PKK76_03205"
     CDS             complement(<1..383)
                     /locus_tag="PKK76_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67502.1"
                     /translation="MQRWEIQEPQAPPLTEGVFRLLVLFSLAFGIEWIAAAGFQRPID
                     SLALSFGPDFQPTQILTHIFLVIPPGFFGGLSLFFLCLMLWGFGGDIERLWGPRHFLN
                     LFLAGMAGSILLGLIAHLSAFPGLSL"
     gene            431..2887
                     /locus_tag="PKK76_03210"
     CDS             431..2887
                     /locus_tag="PKK76_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNL67503.1"
                     /translation="MSANLTDKQKKIILGTEKTLFISLFAGAILGGLILGLAVREVAR
                     ARDLILVSAYRPSTPTRLYDKNGKVFAELFRQRQELVQLQDIPPHVIQAFLSVEDDNF
                     FNHIGLDVSGILRAAVKNAISGKVRQGGSTLTQQVAKQIYLNAEGKRNRTIRQKIRET
                     LLAFEIEENYTKYEILEVFFNVIYLGHGCKGIACASRLYFNKKVTDLTLAEAALLARM
                     PKSPVEYSPFKNPDECMRQNKIVLRQMADHGYLPKDQVEAVHKKFWDEYWPRVVVQSP
                     SQNIWADRLDKAPFFTDYVRQILEASEEVGPELLYTGGLKIYTTLDLDHQRIAEEELL
                     KKVAEVNQTSRAYAKAGGKGGVDMSLFGIFSQLSMILPVGSPDVKGLDIRGQFRKAYE
                     EEMIEPTQLLGYSTPAFNEAAAFEEFRKATISYTTNLQVQGAFISIEPRSGYITSMVG
                     GTEFTPQNQFNRALQARRQPGSAFKIFIYGAAFEKRLISTQSAVNDAPFYSIDAVGNN
                     WSPGNYDEGFRGLTPVFAAFALSINTCAIQVYYKVGPDAIIEFAQRLTKITNKDRFHP
                     DPSLALGSSEVTPFEMAKAVAVIGNDGKDVIPFAVRYVTDQSGNTLYNQEENIRKIIK
                     AKTDENRIQIIDPALAYLLRKIMIAVADHGTATLGVRQTAQFTGSLAAKTGTTSSWSD
                     AWIVGFNPDVAAAIWFGFDKSSVTLGAGQAGGHIAAPTMGRFYKRIYDEKHLKPPMFS
                     DRPDGDRPPPGIVTAGCGGEAMGPVQIGNKTLQVPGDVACAGEETRIYDQRELLMKDL
                     GIKPEDLGIQGNVRFKNEKQ"
     gene            2917..4074
                     /locus_tag="PKK76_03215"
     CDS             2917..4074
                     /locus_tag="PKK76_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNL67504.1"
                     /translation="MKSTQIGKDRREGQFAQLLEASLKNQTKVEAGSYDLVTVSDITN
                     KDFVFVQSPRGTGLIPREELLDHHGKIRVSRGEKIPAFYRSTENGEMMFTTQPSGRAK
                     MDIVRTAIENKIPMTGRILKKIKGGFEVVIGEVNAFCPASHMDGNPDVKSEVTFLITE
                     AKGDRVIASNRAFRDIEKKRQKDVLMQSLQPGDVVEGTVANLADFGAFIDIGGMEGLV
                     PLSEIAHQRVTHPSQVLKSGQSVRVKILQIDWKDDRLTLSLRALLENPWQGQLPWKEG
                     DIVEGKVESIKPFGVFVKLEGNFTGLIPAGESGVARGQPFEKFFQKGQTLRVMVHTID
                     REREKISLSVRGVAAHDQEQEFAEYLKVQGPQKEELSSFGQQLRDAMNKKK"
     gene            4123..6828
                     /locus_tag="PKK76_03220"
     CDS             4123..6828
                     /locus_tag="PKK76_03220"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNL67505.1"
                     /translation="MNEPLSKPHALSTDQILELVTSSRTGLSVAEADRRLALWGPNEI
                     PTQKRSSWIRILAQQLKNVLIIILLLATVLSAVLGHSTEAAVIAIIVIFAALLGFIQE
                     FRAEKAMSALKQLSAPTALALRDGEVQKIAARNVVPGDIIHLHAGDRIPADARILESF
                     SLRTEEASLTGESLPVEKETMALAEDAALADRVNMVYSGTSVSYGRGVVVVTNTGLQT
                     EFGKIARMLESVEHARTPLQINLDKVGNALAKAAGAIVVLITILGVMRGQPFLDMILF
                     GIALAVAVVPEALPAVVTVSLALGVQRMIKRNALVRRLPAVETLGSVSVIGSDKTGTL
                     TKDEMTVRKIHLPDQRFDVTGSGYEPSGDFVGNGTSVSPSPELILALKAASLASDASL
                     ENKDGSWKITGDPTEGAMIVAAAKAGLDKDSLDNEFPRIHENPFTSETKRMTTLHSEG
                     TSKTAYSKGAPESILPSCTHYLSQKGILALTDREKSEYLKEAAFLAGEALRVIAVAMK
                     QNANEENAEEGMIFLGLLGMIDPPRTEVKTAIEKCRRAGIKPIMITGDHPITAAAIAR
                     ELGILRNGKVITGVELDSMSPEEFKRQVESIEVYARVSPAHKLMVVHAFQKNGHSIAM
                     TGDGVNDAPALKKADIGIAMGITGTDVTREAAAMTLTDDNFASIVAAVEEGRGVFGNI
                     KKYLMYLLSSNIGEIGLMAGTSMLGLPLPLSAVQILYVNLATDGLPALALAVDPPDED
                     AMNQPPRNASAGIFTRPVVVLMLAGGIWSTLVNLGLFAWAQSSGRSLSEAMTMSFASL
                     VLIQFFKAYNYRSDQASVWHKPFANKWLNMAIAWELLLLSLVIYHPFLQKAFGTYAIT
                     AVDLVVILSLALSIIPVLETVKWLVRRGWFAQKQAAGAKPATHER"
     gene            complement(6825..7166)
                     /locus_tag="PKK76_03225"
     CDS             complement(6825..7166)
                     /locus_tag="PKK76_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67506.1"
                     /translation="MAQSAQFETIARDLVNVGIALFQITADRIENLQKDIVAGYSGLV
                     TRGAQDKSQMAESLRERLSQSLNVVQTVQDSLGVAVQKLPANRSVPVNTPVATPIDSA
                     KKTAAKKKKSA"
     gene            7276..8508
                     /locus_tag="PKK76_03230"
     CDS             7276..8508
                     /locus_tag="PKK76_03230"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL67507.1"
                     /translation="MRKICGLLILLLSSCARQFDDNTDFLTRSIVAYDVFRLIDIPRF
                     AYAPLSGGNIGGFSVNRVTGQITLVGTYSTGISPSALVTDTQGKFAYATGGTTLYAAS
                     IDQTTGALVSAGTQATGAAPSAPRMDPYNRFVYLISGAGSVTGYAINQTSGATTLIGS
                     WPSAVGYGKPLVHTSGRYLYANTGSGIYMYSIDQTSGALTSLGSISDGGIAGQMELDS
                     TGKYLYHTYPGSVTVFSVNSDGTLSNIQTVAGLAAPSSLNLEHPFKRFAYLTSLTGGT
                     TVMMYQVSNGALSLLGSVAGGTNTGAIRIEPSGRFAYLCNQGSGDVTQYSIDQFTGLL
                     YSFGSVVSGSGPIIASPDPTGRFLYSPNATGASINQYSIDPFSGQLRLIQNTPTSGNP
                     SYVGLVSYPASAIYQYYY"
     gene            complement(8514..>10404)
                     /gene="polA"
                     /locus_tag="PKK76_03235"
     CDS             complement(8514..>10404)
                     /gene="polA"
                     /locus_tag="PKK76_03235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNL67508.1"
                     /translation="KGALDQGTLFEEEPEQRMFSGKEHFLGTDVEYEMVTDAEQLKRA
                     LREHNRSVVVVDTETDSADPMRARLVGISISAREKKATYIPLPPPGSPYESAGLALNE
                     VRSLLKEFFGEKTRSFVGQNIKYDYTVLRRHGMPLNAVAFDTMLAGYLLNPNIRRNSL
                     DDLALDYLDYSTIRYEDVVGTGRKQVTMDQLPPANIRDYACEDADLTLRLHQVLGRKL
                     KENKLLEINDSLEVPLIPVLADMEVAGMAIDLQYFKELSRDYKQKITKLEKKIFEEAG
                     QVFNVNSTRELQFVLIEKLKLPMAKKIKTGFSTDQKELDRMRGMHPMIDHLLEYRKYT
                     KLLGTYIDALPELVHPETGKLHTNFHQAVAATGRLSSMDPNLQNIPVREDTGKAIRRG
                     FVCSKGRVLLSLDYSQVELRIMAHYSHDPALMEAFTRDDVDIHARTASSIFGVAEDQV
                     TADMRSKAKVVNFSIIYGVTDFGLSQSLQVPRAEAKMYIEKFFEKYPGVKKYMDDTIA
                     FAGKNGYVETLFGRRRQIPDIHSPNRFRREGAERTAINTPIQGTSADLIKRAMIDIHA
                     KMKSKKLESQMILQVHDELLFDVVPSEKDEVVSIAKGEMESTRLRVPLRVDHGFGQNW
                     DEAKG"
BASE COUNT         2760 a         2682 c         2574 g         2388 t
ORIGIN      
        1 aggctcagcc ctggaaaagc gctcaaatga gcgatcagtc ccagcaggat cgaaccggcc
       61 atccctgcca gaaacaggtt tagaaaatgg cgcggccccc agagcctctc aatgtcccct
      121 ccgaagcccc agagcatcaa acaaaggaaa aagaggctca aaccgccaaa gaacccgggc
      181 ggaatcacta aaaaaatatg tgtgagaatc tgcgttggct ggaaatctgg tccaaaagag
      241 agtgcaagcg agtcaatcgg cctttgaaag ccggctgccg cgatccattc aataccaaaa
      301 gcgagagaaa atagaaccag cagccgaaaa actccctctg ttaatggagg ggcttgcggt
      361 tcctgaattt cccagcgttg cataagcgct ttaaagcagg accgccagaa tccggcaagc
      421 caatcccgaa atgagcgcaa acctgacaga caaacaaaag aagatcatcc tcggcacgga
      481 gaagacgcta ttcatcagcc tcttcgccgg agcgattctc ggcggcctga ttcttggcct
      541 ggcggtccgc gaagtagcca gagccaggga tttgatcctg gtttccgctt acagaccgtc
      601 cacacccaca agactctacg acaagaatgg caaggtgttt gcagaactct ttcgccagag
      661 acaggaactg gtccaactcc aggacattcc gcctcatgta atccaggcgt ttctatccgt
      721 agaagatgat aacttcttca accacattgg tctggatgta tccggcattc ttcgcgcagc
      781 agtgaaaaac gcaatctctg gaaaagtgag acagggtgga tccactctta cacagcaggt
      841 ggccaagcaa atctatctga acgcggaagg taaaagaaat agaacgattc gccagaagat
      901 acgcgaaact ctgctcgcct tcgaaataga agagaactat acaaagtacg aaattctaga
      961 agtattcttc aatgtaatct atctgggaca cggatgcaaa gggatcgctt gtgcatcgcg
     1021 tctctacttc aataagaaag taacggacct cactctcgca gaggcagcgc tccttgcgcg
     1081 tatgcccaag tctcctgttg aatattctcc gttcaagaat cctgacgaat gcatgcgaca
     1141 aaacaaaatc gtactgcggc agatggcaga tcacggctat cttccaaaag atcaagtgga
     1201 agcagtgcat aaaaaattct gggatgaata ctggcccagg gttgtggttc aatctccttc
     1261 gcaaaacatc tgggcggatc gcctggacaa agcgccattc tttacggact atgttcgcca
     1321 gattctagaa gcatccgaag aagtgggacc agagcttctc tacacgggtg ggttgaaaat
     1381 ctacaccaca ctggatctgg atcatcaaag aatcgcagaa gaagaattac taaaaaaagt
     1441 tgcggaagta aaccagacca gccgcgcgta cgcgaaagcg ggcggaaaag gcggagtgga
     1501 catgagtcta ttcggcattt tcagtcagct gtccatgatt ttgcctgtgg gatcgcctga
     1561 cgtgaaaggt ctggatattc gcgggcaatt tcgcaaagca tacgaagaag aaatgatcga
     1621 gcctacacag ctgcttggtt acagcactcc tgcattcaat gaagctgctg ctttcgaaga
     1681 gtttagaaaa gcaaccatca gttatacgac caaccttcag gtgcagggcg cattcatttc
     1741 gattgaacct cgcagcggtt acatcacttc catggtggga ggaactgagt tcactcctca
     1801 gaatcaattc aaccgtgcat tgcaggcgcg caggcaacct gggagtgcat tcaaaatctt
     1861 catttatggc gcagcatttg agaagcgtct catcagcaca caatccgcag tcaacgatgc
     1921 tcctttctac agcattgatg ctgtgggaaa caactggtct cctggtaact acgacgaagg
     1981 gttccgcggt ctaacgccag tatttgctgc tttcgctcta tccatcaaca cgtgtgcgat
     2041 tcaggtttat tacaaagtag gtccggacgc gatcatcgaa ttcgcacaga gactcacaaa
     2101 gatcacaaac aaagatcgat ttcatccaga tccatctctt gcgctcggtt caagcgaagt
     2161 cactccattt gaaatggcaa aggcagtcgc agtcatagga aatgatggca aagatgtcat
     2221 tccatttgcg gtgcgatacg taacggatca aagcggaaac actctctaca atcaggaaga
     2281 gaacatccgc aaaatcatca aagcaaagac agacgaaaac cgcattcaga tcattgaccc
     2341 cgcgctcgca tacttgctac gcaagatcat gattgctgtt gcggatcacg gaacagcaac
     2401 gctgggcgta agacaaacgg cacaattcac tggaagtctg gctgcgaaaa caggaactac
     2461 ttctagctgg tcagatgcgt ggatcgttgg attcaatccg gatgttgccg cggccatctg
     2521 gtttggtttt gataaatcga gcgtcacact cggcgcaggc caggcgggcg gtcacattgc
     2581 cgctcctacc atgggtcgat tctataaacg tatctacgac gaaaaacatc tcaagccacc
     2641 catgttctca gatagaccgg atggagatcg tcctcctcct ggaatcgtaa cagcaggttg
     2701 cggtggagaa gcaatgggac cggtgcaaat cggaaacaaa actcttcagg tccccggcga
     2761 tgtagcatgc gcgggagaag agacacgcat ttatgatcag agagagcttt tgatgaagga
     2821 cctgggcatt aagccagagg acctcggcat tcagggcaat gtccgattca agaatgaaaa
     2881 gcaataattc ccgcgtcgtc gggagggaga atcgttatga agagcacaca aatcgggaaa
     2941 gacagacgcg aaggtcaatt cgcacagctg ctcgaagcaa gcctgaaaaa ccagactaaa
     3001 gtcgaagctg gaagttacga cctggtaact gtatctgaca tcacaaacaa agatttcgtg
     3061 tttgttcaat cacccagagg gaccgggctc attccgcgcg aagagcttct cgatcatcat
     3121 ggcaagatcc gcgtttcgcg cggtgagaag attccagcat tctatcgctc cacagaaaac
     3181 ggagaaatga tgttcacaac gcagccttca ggccgcgcga aaatggacat tgtccgcacc
     3241 gcgattgaaa acaaaatccc aatgacagga cgtatcctca agaaaatcaa aggcggattt
     3301 gaagttgtca ttggagaagt aaacgcgttc tgccccgcat ctcacatgga cggaaatcca
     3361 gacgtaaagt cagaagttac ctttctgatc acagaagcaa agggggaccg agtcatcgca
     3421 tctaacagag cgtttcgcga catcgaaaag aagcgccaaa aagatgtgct catgcagtca
     3481 ctccagccag gggatgtagt ggaaggaacc gtggccaacc tcgcagactt tggcgcgttt
     3541 attgacattg gcggaatgga aggactggtt ccactttcag agatcgccca ccagagagtg
     3601 actcatccat cccaggttct caaatcggga cagagcgttc gcgtaaaaat cctgcaaatc
     3661 gattggaaag acgatcgcct cactctctct cttcgcgcac tcctcgaaaa cccatggcag
     3721 ggtcagcttc cctggaaaga aggagatatt gtagaaggca aagtggaaag catcaaacct
     3781 tttggagtat tcgtaaagct agaaggaaac ttcacaggac tgattccagc aggagaatca
     3841 ggagtggcac gtggacagcc ttttgaaaag tttttccaga aaggacaaac tcttcgcgtc
     3901 atggtgcaca ccatcgaccg cgaacgcgaa aagatttccc tttccgtgcg cggagtcgcg
     3961 gcacatgacc aggaacagga attcgcagag tacttaaaag ttcagggccc gcagaaagag
     4021 gagctctcca gttttggtca gcagcttcgc gatgcaatga acaagaagaa ataaagcaca
     4081 cctgcgcgtg gccttcgcgc gcgcccttcg gggccagcca tcatgaacga gccactctca
     4141 aagccccatg ccctgagcac tgatcaaatc ctggagctcg tgacctcatc gcggaccgga
     4201 ctctccgttg cagaagccga tcgtcgcctg gccctctggg gcccaaacga aatccccact
     4261 caaaaaagat cctcctggat tcgcatcctg gcccaacagc tgaagaatgt gctcatcatc
     4321 attctacttc ttgccacagt tctttctgcc gtacttggcc atagcactga agcagcagtc
     4381 atcgcaatca ttgtaatctt tgccgcgctt ctcggattta ttcaagagtt tcgggcagaa
     4441 aaagcaatgt ccgctctcaa acaattgagt gctcccacag cactcgcatt gcgtgacgga
     4501 gaagtgcaaa aaatcgccgc gcgcaacgta gtaccaggag acatcattca ccttcacgcc
     4561 ggtgaccgaa tccccgcaga cgcacgaatt ctagagagct tcagtcttcg cacagaagaa
     4621 gcatcgctaa ccggagaatc actacccgta gaaaaagaaa cgatggcttt ggcggaagac
     4681 gcagcactcg cggatcgggt aaacatggta tactccggaa caagcgtgtc ttatggacga
     4741 ggtgttgttg ttgtgacgaa cacgggcctg cagactgaat ttggaaaaat cgcccgcatg
     4801 cttgaatccg tggagcacgc gcgcactcct ctgcaaatca atctggataa agtgggcaat
     4861 gcgctggcaa aggcagcggg cgctattgta gtattgatca cgatcctggg agttatgcga
     4921 ggccagccct tcctcgatat gatcctgttt gggattgcac tggcggtggc tgtggttcca
     4981 gaggctttgc ctgctgtggt tacagtgtct ctggctctgg gcgtgcaaag aatgatcaag
     5041 cgcaacgcgc tcgtgcgaag gttgccggca gtcgaaaccc tgggtagcgt ttccgtgatt
     5101 ggctccgaca aaacgggaac gctcacaaaa gatgaaatga ccgtcagaaa gatccatcta
     5161 ccggatcagc gattcgatgt cacagggtcc ggatatgaac cgtcaggcga ctttgtgggc
     5221 aatggaacaa gtgtctctcc gtcgccggaa ctcattttag ctctcaaagc cgcttctctt
     5281 gcatcggatg cttccctgga aaacaaagac ggatcctgga agatcactgg agatcccacg
     5341 gaaggggcca tgatcgtggc cgccgcgaag gcgggcctgg acaaagattc actggacaac
     5401 gagttccctc gcattcacga aaacccgttc acgtctgaaa caaaaaggat gaccacgctg
     5461 cacagcgaag gcaccagcaa aactgcctac tcaaaaggcg ctccagagtc gattctacct
     5521 tcctgcactc attacctctc acaaaaggga attctggcac tcacagacag ggagaagagt
     5581 gaatatctca aagaggcagc ctttctcgca ggcgaagcgc tcagagtaat cgcggttgcc
     5641 atgaagcaga acgcaaacga agaaaatgca gaagaaggaa tgatttttct ggggcttctt
     5701 gggatgattg atccgcccag aacagaagta aaaacagcga ttgagaaatg tcgtcgcgca
     5761 gggatcaaac caattatgat cactggcgat catccaatca ccgcggccgc catcgcacgc
     5821 gaactgggca tactgagaaa tggcaaagtg atcacgggag tcgaactcga ctcgatgagc
     5881 cccgaagaat tcaaaagaca ggttgagtca atcgaagtgt atgccagagt ttcgcctgcg
     5941 cacaaactca tggttgtgca tgcatttcaa aaaaacggac attcaatcgc aatgactggc
     6001 gacggcgtca atgacgctcc agcgcttaag aaagcggata ttggaatcgc catgggaatc
     6061 acgggcacag acgtaacgcg cgaagcggct gcgatgactc tgaccgacga caactttgcc
     6121 tccatcgtgg cagcagtgga agaaggaaga ggcgtattcg gaaacatcaa gaagtatctg
     6181 atgtatcttc tttcctcgaa cataggagaa atcggactca tggccggtac atccatgctt
     6241 ggattgcccc ttccactgtc cgcagttcaa atcctgtacg tgaacctggc aacagacggc
     6301 ctgccggcac ttgcacttgc agtggatcca ccggacgaag atgccatgaa ccaacctccg
     6361 agaaacgctt ccgctggaat cttcactcga cctgtagttg tcttgatgct cgcaggtgga
     6421 atctggtcca ctctcgtgaa tcttggtctg tttgcgtggg cgcagagttc gggcagaagt
     6481 ctttcagagg caatgaccat gtcctttgct tctctggttc tgatccagtt cttcaaagca
     6541 tataattatc gttcggatca ggcgagcgtg tggcacaagc cattcgcgaa caaatggctg
     6601 aatatggcga tcgcctggga gctgctcctt ctgagcctgg tgatttatca tcccttctta
     6661 caaaaagcat ttggaacgta tgcgattacg gccgtggacc tggtggtaat tctatccctt
     6721 gcgctttcca tcataccggt tctggaaacc gtgaaatggc tggtgagacg gggctggttt
     6781 gcccaaaagc aggcagccgg cgcgaagccg gccacgcatg aacgttaagc gctcttcttt
     6841 ttctttgctg ctgttttctt tgcagaatca ataggagtgg ctacaggcgt attcactgga
     6901 acagaacgat tcgctggcag cttctgcacc gcaacaccca ggctatcctg gacggtttga
     6961 acaacattca gactctggct gagtctttcg cgcaggcttt ctgccatctg gctcttgtcc
     7021 tgagcaccgc gcgttacaag tccggagtag ccggccacaa tgtctttctg aagattttca
     7081 atacggtctg ccgtgatttg aaaaagggca atgcctacat ttacgagatc acgtgcgata
     7141 gtttcgaact gagctgattg agccatggac aatcttttcc cctgtacgga ggctttcaag
     7201 tcaaacttga aggccgttta gggctggaca agcccccgcg aggagccaaa tcaagttgtg
     7261 aacttgatag gtcgcatgcg aaagatatgc gggttactga tactactatt gtcatcttgc
     7321 gcccgccagt tcgatgacaa cacggacttc ctcacgcggt ccattgtcgc ctacgacgta
     7381 ttcagactca tcgacatacc acgcttcgcg tatgctcctc ttagcggtgg aaacattgga
     7441 gggttctcag taaaccgcgt aacgggacag atcacacttg ttggaaccta ttctacaggg
     7501 atcagtcctt ccgcactcgt aacagacaca cagggaaagt tcgcatatgc aaccggtgga
     7561 acaactctct acgcggccag tattgatcag accacaggcg cgcttgtaag cgcgggtacg
     7621 caagcaaccg gcgctgcgcc gagcgcaccg cgaatggatc cttacaatcg cttcgtctac
     7681 ctcatcagtg gcgccggaag cgtaacaggc tatgcaatca atcagaccag cggtgcgacg
     7741 actctgattg gatcgtggcc ttccgctgtt gggtacggaa agccactcgt tcatacaagc
     7801 ggtcgatatc tttatgcgaa cactggctct ggcatctata tgtattccat tgatcagact
     7861 tcgggggcac tcacaagcct gggatccatc agcgacggcg ggatcgctgg acaaatggaa
     7921 ctggattcaa ctggaaaata tctctaccat acgtatccag gatctgttac tgtgttctcc
     7981 gtcaattcag atggaacgct ttccaacatc cagacagtgg ctggactggc ggcgccttct
     8041 tctctcaatc tggaacaccc cttcaaacga tttgcctacc ttacaagcct tacaggtgga
     8101 acgacggtga tgatgtatca ggtttccaat ggcgcactga gtttacttgg ttctgttgcc
     8161 ggcggcacga acaccggagc cattcgcatc gagccttccg gaaggtttgc ttatctttgc
     8221 aaccagggtt caggagatgt cacacagtat tccattgatc agtttactgg tttgctgtac
     8281 tcgttcggga gcgtcgtaag cgggagcgga ccaatcatcg catcgcctga tccaaccgga
     8341 cggttcttgt attcgccaaa tgcaacggga gcaagcatca accagtactc gattgatccg
     8401 ttttccgggc agctacgcct cattcaaaat acacctacat ctggaaatcc aagttacgtt
     8461 ggactcgttt cctatccggc ttccgcaatt tatcaatact actactgaat cgcctaaccc
     8521 ttcgcttcgt cccagttttg tccaaatcca tgatccacgc gaaggggcac gcgaagcctt
     8581 gtgctttcca tttccccttt tgcgatcgaa acgacttcat ccttttcaga gggcacaaca
     8641 tcaaacagaa gttcgtcgtg cacttgcaga atcatctgag actcaagttt ctttgatttc
     8701 atcttcgcgt gaatatcaat catcgcacgc ttgatcaggt ctgcactggt tccctggatc
     8761 ggcgtattga tggctgttct ttccgctccc tcccgacgga atcggttggg ggaatgtatg
     8821 tctggaattt gcctgcggcg accgaataac gtctccacat atccgttctt tcctgcaaat
     8881 gcgatggtat catccatgta ctttttgacg ccggggtatt tctcaaaaaa cttctcgatg
     8941 tacatttttg cttctgctcg cggcacctga agactctgcg aaagaccaaa atcggttacc
     9001 ccataaatga tcgagaagtt cacaaccttt gctttggatc gcatatctgc tgttacctga
     9061 tcttctgcaa ctccaaagat ggaagaagct gttcgcgcgt gaatgtctac atcgtcgcgc
     9121 gtgaaggctt ccatcaaagc cgggtcatgc gaataatgcg ccatgatgcg cagctccacc
     9181 tgagaataat cgagagacaa aagcaccctg cccttgctac aaacaaaacc tcggcgaatc
     9241 gccttgcctg tatcctctcg cacaggaata ttctgcagat tgggatccat agatgacaga
     9301 cgaccagttg cggccactgc ctgatgaaag ttcgtatgaa gcttgcctgt ttctggatga
     9361 acaagctcag gaagagcgtc aatgtatgtg cccagaagtt tggtgtactt tctatattca
     9421 agaagatgat cgatcatcgg atgcattccg cgcatgcgat cgagttcttt ctgatccgta
     9481 gaaaagccag tcttgatctt ctttgccatg ggtagcttca atttctcaat caagacaaat
     9541 tgaagttcgc gagttgagtt cacattgaag acctgacccg cctcctcgaa gatcttcttt
     9601 tccagctttg tgatcttctg cttatagtcg cgcgaaagtt ccttgaagta ctgcaaatcg
     9661 atggccatgc ccgccacttc catatccgcc agtacgggaa tcagaggaac ttcgagcgaa
     9721 tcattgattt caagaagttt gttctccttg agcttacgcc cgagcacctg atggaggcga
     9781 agagtcaaat ccgcatcttc gcacgcatag tctctgatat ttgccggtgg aagctgatcc
     9841 attgtcactt gttttctgcc cgtaccaacc acatcttcgt agcgaatcgt agaatagtct
     9901 agatagtcca gcgccaggtc atccaggctg tttcttctga tattcggatt gagcagataa
     9961 cccgcaagca tagtatcgaa agccacagca ttgagaggca ttccatgcct gcgcagtaca
    10021 gtgtaatcgt acttgatatt ttgccccaca aaggagcgtg ttttttcccc aaagaattct
    10081 ttgaggagtg accggacttc attcaatgca agccctgccg attcgtatgg ggaaccagga
    10141 ggtggaagtg gaatgtaggt tgccttcttt tcgcgggcag aaattgagat tccaaccaga
    10201 cgcgcacgca ttggatctgc ggaatctgtt tccgtatcca cgaccacaac agaacgattg
    10261 tgttcgcgga gcgctcgctt caattgctct gcatctgtaa ccatttcata ttctacatct
    10321 gtcccgagaa aatgctcttt gccagaaaac attctttgct ctggctcttc ttcgaagaga
    10381 gttccctgat cgagtgcgcc cttc
//
LOCUS       DAORFF010000047        10342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2958_length_10342_cov_3.753572, whole genome shotgun sequence.
ACCESSION   DAORFF010000047 DAORFF010000000
VERSION     DAORFF010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2958_length_10342_cov_3.753572"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PKK76_03240"
     CDS             complement(<1..506)
                     /locus_tag="PKK76_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL67509.1"
                     /translation="MHSNPRILVVEDSASVQAYLGALLQHAGYHDPIMKQSIEEVLEW
                     LQDLTPEQTPDLVLTDVHLPGKDGIEGLRILKETPVFFEVPFIVMTADDSDDIVGRAF
                     KEGAMDFVRKPFRNVELVARIGAALQLKSQIERRRDRERELLELIEQMETANIDLGVK
                     SRMDYLTGA"
     gene            complement(552..905)
                     /locus_tag="PKK76_03245"
     CDS             complement(552..905)
                     /locus_tag="PKK76_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67510.1"
                     /translation="MQEYLPFFTALAGAAIGGTISILVQIINQRHQIKIIQLQQKTDF
                     AAEETARYFLSHRIHIERSFETIRKSIGGFEEDELRKILVRAGAIRSYREDGSEWWKL
                     LSRMDEYLEKKLDRT"
     gene            complement(949..1839)
                     /locus_tag="PKK76_03250"
     CDS             complement(949..1839)
                     /locus_tag="PKK76_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNL67511.1"
                     /translation="MDVCFADSSQTQLAFERDVYNAAGNSAFWIRIPTLPSGSTTVYM
                     YYNSQSTCPSTGSVWDSNFRAVYHLSSDPSASVPDSANSNALTGIAMTAANLTSTQTG
                     YGFLLDGTTQYLTAPDSPTLQIASSLSLEVVYKLSAYTLNAHYLVEKGDSDFDNYSLY
                     LKRTGAFGCTSPIGCAAFEFRDAGSSYFDITGARDVTPGTWHTLAANVDATGGKVDFY
                     LDGSFSESLPASLPGNMWPQVLSIGRQSFGASSFLFSGTMDEVRISSTLRSADYFAFV
                     HDNVTAGSTPTIGAEETQPF"
     gene            2215..2970
                     /locus_tag="PKK76_03255"
     CDS             2215..2970
                     /locus_tag="PKK76_03255"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HNL67512.1"
                     /translation="MTRDQSFLFLSSVACICFCGFAAVDISTLSSDEAFSRFLKFSSI
                     VACAVIAWTRPGPDRRDTVLLRIAFLLTVLADFLLGVLHLFIPGVAVFALVHIVYVMR
                     HLRGISSLVAEGILAGFCLVFAIVIMRLSSPAMARAGLLHESIAYAVPLTFSLYAGIG
                     VMIRDHFRMVHRIRIAAGIVLFFAVDIAVAHFQALPDGLMRTILGMAIWPLYLPSQFL
                     LACSGSGAKQGVPVCVSVPRPGPSNAFLRLRLD"
     gene            3039..4481
                     /locus_tag="PKK76_03260"
     CDS             3039..4481
                     /locus_tag="PKK76_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNL67513.1"
                     /translation="MSEVKKIKVKINQVEYEVPANWSIIQACHNNGVEVPHYCYHQDL
                     SVAGNCRMCLVKASNAPRPVIACATPLAENMEIDTLSEDVVAARKSVLEFLLINHPLD
                     CPECDQAGECRLQDYSYEYGRDHGRFHEEKNIRAKATLGPHVKYWGSRCIVCTRCVRF
                     TDEISGTGELTVAQRGDRSEITVFPGQTLDNPLSLNTVDVCPVGALVSADFLYRSRVW
                     FMHSTPSVCADCSLGCNTRVDADKQNKIKRIIPRRNNEVNKEWMCDHGRLSFPYVYEN
                     RMNLPTKNNQEIPWADAFRMAAEALGGNDVVFLVSLWTTLEGMEKIKSLAAEKGSPVF
                     GFGNASTKDQVFPGFTIYGDKNPNRAGFTATFGDLKSPSASDLNGKTVFVFGNVPRFE
                     IPADLAQSLTSASKLIVVDFARGSFANHAKTQLVLSGLTHFEKNGTFVNRGGLRQGFS
                     PVIEPVGFGRSEVELLDGILKERGKTAAAR"
     gene            4488..5516
                     /gene="nuoH"
                     /locus_tag="PKK76_03265"
     CDS             4488..5516
                     /gene="nuoH"
                     /locus_tag="PKK76_03265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNL67514.1"
                     /translation="MEQLVFIVFKCVTAVVLVLTAVPLMIWMERKVSADFQLRIGPNR
                     MGPAGVFQLPVDAVKLLFKESFAPRGIDRFMYLAAPVVAFVPGLLNFAVIPAGEIRAE
                     QGGLLLAVSDIELGLITILAISSLTSYGIAYAGWASGNQFSLLGGVRSCAQLISYEIV
                     LGLSVLSVIMMSGTLNLSEIVRMQNQLRFGWMPSWNIALLPLTFILFLIAAFAETNRA
                     PFDLPEAEQELVGGYHTEYTGLRYAWFMFGEYAGMFTMCSLITTLFLGGWMLPGLTLP
                     LGILGGILGFGIFLTKVIALIFFFMWVRWTLPRLRWDQLMRFGWKRLIPAGLINLVIT
                     AVLVQVWK"
     gene            5530..6060
                     /locus_tag="PKK76_03270"
     CDS             5530..6060
                     /locus_tag="PKK76_03270"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNL67515.1"
                     /translation="MKQAVPYATTLGSIKALIQGLYVSMGQFLKKPVTIQYPEVQAPI
                     DPRFRGEHALTKDEFGHMKCVACYMCATACPAECIHIEAQPAPEGEGWEGRDKIPQSF
                     DIDMLRCIYCGYCVEACPKDAIMMTNKVPVVHDNRKAFIYGIDKLLGNFEEYGRDLHT
                     KQLKERGIEEPGHAGH"
     gene            6047..6607
                     /locus_tag="PKK76_03275"
     CDS             6047..6607
                     /locus_tag="PKK76_03275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNL67516.1"
                     /translation="MQVTEIIFLINAAFLVLFSLAVVVIKNPVRATFMLILSFLPTTA
                     IYFFLHAPFVGILQILVYAGAILVLFTFVVMMVNPAPGDGEISPDGAKRLPRSRIAAV
                     GIVLLLGGVLLSVVLSISFPAIASVPAEFGGLRAIGTMIFKNPSENPYIFSFELLSFL
                     VLAGIIAAVSIARGREHHRHGPGKPQ"
     gene            6604..6936
                     /gene="nuoK"
                     /locus_tag="PKK76_03280"
     CDS             6604..6936
                     /gene="nuoK"
                     /locus_tag="PKK76_03280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNL67517.1"
                     /translation="MSVFSLQTCLILSSLLILIGCYGIIIRRSLLLIFLSVEIVLNGV
                     HVALVAFNYFRWSGLEAGHYIYMLSIGVAAVEAAVGLSMIILIFKNSGTILSDALTKL
                     GEDKEENK"
     gene            6933..8846
                     /locus_tag="PKK76_03285"
     CDS             6933..8846
                     /locus_tag="PKK76_03285"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNL67518.1"
                     /translation="MNAALPWIPLLPALGAGLCAIGGRRLGLRGSSFIVVTASLVSLV
                     LCCLIAFQGVIAGNIFQEGKSEVFNAGPFLQFSEEWSALLGLRMDLMSGLMSLLVTLF
                     GTLILMFSIDYMREDDSPVRYFASLSLFISAMLILVLSENLVLLFLGWEGVGLCSYLL
                     IGHEWKKSGVPLAAYRAFFVNRIGDLFFILGVFLLLKTLGTVSFSGMSQKFETMSGSL
                     DPLARRDLLMASFLLLGGAFGKSAQVPLHVWLPDAMAGPTPVSALIHAATMVTAGVFL
                     VARLNWLYTAIPEARGLLLICGSLTLLVGAVLACFQSDIKKVLAYSTLSHLGLMFIAL
                     AAGSVSAGLFHLFGHASFKAISFLAAGSIILAAHHEQDLHKLRGSLKHLPVTRVALWT
                     GAIGGAGLVPFLASGFFSKEAVLHSISEASFGVFGLTISGGMFYWFVFGAELLGVVYL
                     FRMLALLESKPESHESHASHAIEEKGVFIRAVLAILVLVAIAFGVASSPFVSGFSHFL
                     FDKAEHVSIVDICLASGSSILVAIIVFVVFRADAAEAVVGTGARSGLIRNLFYFDKIY
                     SAVLLAPLGAIGSATARFIEVPIFQGALRGGAAVSRTVSSAFSRMQKGLISQYAVIMI
                     VSAAIVMLLALLA"
     gene            8851..10296
                     /locus_tag="PKK76_03290"
     CDS             8851..10296
                     /locus_tag="PKK76_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNL67519.1"
                     /translation="MELLTIIAFPVLAFVVHSFLPKQRGAFLVSTLFYSALLVVQSLR
                     AFISPVYLNAGGVRLWGDFALVPSFYLTGLNAPLLLLIGLVLPFVALLLRSVHAEDKR
                     YTLSAWVFLLGLAGTFISQSLILFYVFFETSLIGAYFWIGLFGRSSVSGSPGGWSGLT
                     RFFLFTLVGSLSMLASIAALSSGGKDVRFTDVPIVLGTFSDSGRFWIFAGFALAFAIK
                     LPLFGFHGWLRETYAMAPPAARALLSGIMSKLGAYGFLLLAVYFGIEFRLHSKLIIAL
                     CAAGVLYGGLVSLSQKRFLDVVVYSSLAHLNLIVMGLAAMSGSVSDTTIVSACVLQMF
                     NHGLIMAAILVYDARMGSPDESQSGMREHAGILSALLLLSLFAAVSLPGLSSFPPEFA
                     ILYAAFKTSIWIALVAGLGLLISAAALIRSYHRIYAGNFAGSANVASSGEVSRMEAVI
                     GVIFGLVWIGLGILPGLIMSPVAAALGVIKL"
BASE COUNT         2120 a         2534 c         2690 g         2998 t
ORIGIN      
        1 gctccggtga gatagtccat tctggatttt acacccagat caatattggc tgtttccatc
       61 tgctcgatca gttcgaggag ttctcgttcg cggtctcgtc ttcgttcaat ctgcgatttt
      121 agttggagtg ctgctcctat gcgagcaacc agttccacat tacgaaacgg tttgcgcaca
      181 aaatccatgg caccttcttt gaaggccctt ccaacgatgt cgtcactgtc gtctgcagtc
      241 atgacaataa agggaacttc aaagaaaact ggagtttctt ttagaatgcg gagtccttcg
      301 attccgtctt tgcccggcag gtgaacgtcc gtcagaacca gatcgggagt ctgttctgga
      361 gtcagatcct gcaaccactc gagaacttct tcgatggatt gtttcatgat tggatcatgg
      421 tatccagcgt gctggagcaa tgcaccgaga taagcctgga ctgaggccga atcctcaact
      481 accagaattc gtggattgct gtgcatggtt cctccatgtc atgtctccgg cgacatgcaa
      541 ccagaaatca cttaagtgcg atcgagtttc ttttcgaggt actcatccat tctggataag
      601 agcttccacc attcgcttcc atcttccctg taggatcgaa tagcgccagc ccgaactaaa
      661 attttccgca gctcatcttc ttcaaacccg ccgatgctct tccggatcgt ctcaaaggac
      721 ctttcaatat gaatgcgatg gcttagaaag tagcgtgcgg tttcttctgc tgcaaaatct
      781 gtcttttgct gcaattgaat gatcttgatt tgatgtctct ggttgatgat ttgaactaga
      841 atagaaatgg taccgccaat ggcagcgccg gccagagcag tgaagaaagg caagtattcc
      901 tgcatgatcg attttagagc ggcgcgcttt ttgcgcaacc cgaagcaatc agaatggctg
      961 cgtttcttcc gcaccgattg tgggcgtaga accggcggtt acattgtcat ggacaaatgc
     1021 aaaataatcc gcagatcgca aggtggagct gatgcgcact tcgtccatgg tgcccgagaa
     1081 caaaaaggaa gacgcgccaa agctttgtct tcctatagaa agaacctggg gccacatgtt
     1141 tccaggaaga gatgcaggta gagattcgct gaaagatcca tccaggtaga aatccacctt
     1201 tcctccggtg gcgtctacgt tcgcagcgag agtatgccag gttcctggcg tcacatccct
     1261 ggcacccgta atgtcgaagt atgaggaacc ggcgtccctg aactcgaatg cggcgcaacc
     1321 aatcggcgaa gtgcatccga atgctcccgt tctcttcaga tagagagagt agttgtcaaa
     1381 atcactatct cctttttcca ccagatagtg cgcgttcaga gtataggcgc tcagtttata
     1441 tacaacttcc aggctgaggc tggatgcaat ctgaagcgtg ggcgaatccg gcgcggtgag
     1501 atactgcgtg gttccatcca gcaggaatcc ataccccgtt tgagtggaag tgagattcgc
     1561 ggccgtcatc gcaatgccag tgagcgcatt ggagttcgcg ctatccggaa cagaagcgga
     1621 ggggtccgaa ctcagatgat agactgcgcg aaaattggaa tcccatacac tgccggtaga
     1681 cgggcaggtg ctctggctgt tgtagtacat gtagaccgta gttgagccag aaggcagagt
     1741 tggaatacga atccagaagg cagaattacc agccgcgttg tatacgtcgc gttcgaatgc
     1801 cagctgcgtt tgagacgagt ccgcgaaaca aacatccata ccattagcct gcgcattcgc
     1861 ataactaaag ttagacgatg atagtggaat caaaaccgga acatcggaaa gagtggttcc
     1921 atggttggct atagtgacgg atctgcgcag tccccaggat cgattccacc acggggcaat
     1981 gggacgaaat atactcagcc ccagccacgc cgaagtggcg tcatagtgcg cacaattgcc
     2041 gaggagtgtc agtcccagcc aggcgcagag cagaattctc ttacccgaac gtgtgatacc
     2101 ccattccagg cgtttgcatt tgttttacaa ggattttaac tccaatccgt gcgattgcgg
     2161 acccgcgcag ggcgaagcgc aaaatttgtc acattgagca tataatatgc tcgtatgacc
     2221 agagaccaat cgttcctctt tctttccagc gttgcctgta tttgcttttg cggtttcgcg
     2281 gccgtggata tatccaccct ctcttcagat gaagcttttt cgcgctttct gaagttttct
     2341 tctattgtgg catgcgctgt tatagcgtgg accaggccag gcccggacag acgcgatacc
     2401 gttcttcttc gcatcgcatt cctcctgaca gtactcgccg atttcttgct gggtgttctt
     2461 cacctgttca ttcccggagt ggctgttttt gctctggttc acattgtata cgttatgcga
     2521 catttgagag gtatctccag tctggtcgct gaagggattc tggctggatt ttgtctggtg
     2581 ttcgcgattg tgatcatgag attgtcttct ccggcgatgg cccgcgcggg gttgttgcat
     2641 gaatccatag cctacgcggt tcctctcaca ttttcgctct acgccgggat cggagtgatg
     2701 atccgggatc actttcgcat ggtgcatcgt atccgaattg ccgcgggcat tgtactgttt
     2761 tttgccgtcg atattgctgt ggcgcatttt caggcccttc cggacggcct catgcgaacg
     2821 attctgggga tggccatatg gcctctctac cttccatccc aatttctcct ggcctgtagc
     2881 ggatcgggcg cgaagcaagg cgtcccggtt tgcgtttctg tgcctcgacc gggtccttcg
     2941 aacgcctttt tgcgccttcg tctggattga aaaaaagccc ggaagagatc aggaaaatct
     3001 tgccgcatga ccggtcgggg catccttagc tttgagccat gtcggaagtc aaaaagatca
     3061 aggttaagat caaccaggtc gaatacgagg ttcctgcaaa ctggtcgatc attcaggcct
     3121 gccacaacaa cggagtggag gttcctcact attgctatca ccaggaccta tctgttgcag
     3181 gcaactgccg catgtgtctt gtcaaagcat ccaacgctcc gcgtcctgtc atagcctgcg
     3241 ccacacctct cgcggagaat atggaaatcg acactctatc agaagatgtc gtggctgcgc
     3301 gcaaatccgt tctcgaattt ttactgatca atcatcctct tgattgtcct gaatgtgatc
     3361 aagcaggtga atgtcgtctt caagattata gctatgagta cgggcgcgac catggtcgct
     3421 ttcacgaaga aaaaaacatt cgtgcgaaag ccactctggg ccctcacgtg aagtactggg
     3481 gctcacgctg catcgtttgt acacgctgtg ttcgctttac agatgaaatc agcggaaccg
     3541 gagaactcac cgtagcacag aggggagata gaagcgaaat cacagtattc ccaggacaga
     3601 cgctcgacaa tccactctct ctcaatactg tggacgtctg cccggtgggc gcgcttgttt
     3661 ctgcggactt tttgtatcgc tcgcgcgtct ggttcatgca ttctactcca tctgtttgtg
     3721 cggattgttc tctcgggtgc aacactcgtg tggacgcgga caaacaaaat aaaatcaaga
     3781 gaatcattcc tcgtcgcaac aatgaagtaa acaaggaatg gatgtgcgat catggtcgtc
     3841 tcagcttccc atacgtttac gaaaaccgaa tgaacctccc aacaaaaaac aatcaggaga
     3901 tcccctgggc ggatgcgttc cgcatggcag cggaagcgtt aggcggcaat gatgtcgtgt
     3961 ttctagtaag tctctggacc actctcgaag gaatggaaaa gatcaaatcc ctcgcagcgg
     4021 aaaaaggatc acctgtcttt ggctttggca atgcgtccac aaaagatcaa gtcttccctg
     4081 gttttacaat ctacggagac aagaatccaa atcgtgctgg cttcactgcc acatttggag
     4141 atctgaaatc tccttctgca tctgatttga atggaaagac agttttcgta tttggaaatg
     4201 ttccgcggtt tgagattccg gcagatctgg cgcagtctct aacctctgca tcaaagctca
     4261 tcgtggttga ttttgccaga ggctcgtttg caaatcatgc gaagactcaa ctcgttctct
     4321 cagggctcac tcattttgag aagaatggaa catttgtgaa tcgtggtggg ttgcgacagg
     4381 gattttcccc tgtgattgaa cccgttggat ttggcagaag cgaagtggaa ttgctggacg
     4441 gaattttgaa agaaagggga aaaacggctg cggctcgttg atttagtatg gagcagcttg
     4501 tattcatcgt attcaagtgt gtaactgcgg ttgtcctggt cttgactgcc gttcctttga
     4561 tgatctggat ggagcgcaaa gtcagcgctg acttccagtt gcgcattggt ccaaatcgca
     4621 tgggacctgc cggtgtgttt caacttccag tggatgccgt gaaacttttg tttaaggaat
     4681 catttgctcc acgcgggatt gatcgcttca tgtatctggc ggcacctgtg gttgcctttg
     4741 ttcctggtct gctcaatttc gctgtaattc ccgccggaga gattcgtgcg gagcaaggag
     4801 gtcttcttct tgctgtctct gacatcgagc tcgggctcat taccattctt gctatttctt
     4861 cgctaacgtc atacggcatt gcctacgccg gatgggcatc cgggaatcag ttttcccttc
     4921 tgggaggagt tcgatcctgc gcgcaactca tcagctacga aatcgttctt ggtctatctg
     4981 ttctttctgt catcatgatg agcggcacac tcaatctcag cgagattgtt cgcatgcaaa
     5041 atcaacttcg ctttggctgg atgccttcct ggaatattgc tcttcttcca cttactttca
     5101 ttttgttctt gattgccgct ttcgcagaaa ccaaccgcgc gccatttgat cttccggaag
     5161 cggaacagga actcgttggt gggtatcata cagagtacac cggtctgaga tacgcctggt
     5221 tcatgttcgg agaatacgcg ggaatgttta cgatgtgttc cttgatcacc actctgtttc
     5281 ttggtggttg gatgctcccc ggactgactc ttccacttgg gattctgggc gggattctgg
     5341 ggttcggaat ttttctcacc aaggtgattg ctctaatttt ctttttcatg tgggtgcggt
     5401 ggactctacc gcgcttgcgc tgggatcagt tgatgcgttt tggctggaag cgtttgattc
     5461 cggccgggct catcaatctc gtgatcacgg cagtgctcgt tcaggtctgg aagtgaaggc
     5521 ggatcaagta tgaagcaagc agtgccgtat gctacaactc tgggatctat caaagcgctg
     5581 atccagggtc tgtacgtttc catgggtcag tttctgaaga aaccagtcac cattcagtat
     5641 ccagaagttc aggcacccat tgatcccagg tttcgcggag agcatgctct cacgaaagat
     5701 gaatttggcc atatgaagtg tgtggcctgc tacatgtgcg ccactgcctg ccctgctgaa
     5761 tgcattcata tagaagcaca acccgctccc gagggagaag gctgggaagg cagagacaag
     5821 attcctcagt cttttgacat agatatgctt cgctgcattt attgcggcta ctgtgtggaa
     5881 gcatgtccaa aggacgccat catgatgacc aacaaagttc cagtggttca tgacaatcgc
     5941 aaagcattca tctacggaat cgataagctg cttggaaatt tcgaagagta cggtagagac
     6001 cttcacacga agcaactgaa ggaacgaggc attgaggagc caggccatgc aggtcactga
     6061 gatcatcttt ctcatcaacg cggcatttct tgttttgttc tccctcgctg tagtcgtgat
     6121 caagaacccg gtgcgcgcaa cctttatgtt gattctgtcc ttcctgccca ccactgcgat
     6181 ctacttcttt ttgcatgcgc cctttgttgg aattttgcaa atcctcgttt acgcaggcgc
     6241 gattctggtt ctgttcactt ttgttgtcat gatggtgaac cccgcacctg gagacggcga
     6301 aatttcgccg gacggtgcaa agcgcttgcc tcgaagcagg atcgcagctg tgggtattgt
     6361 tctgcttctg ggtggtgtgc ttctgtctgt agttttgagt atttcgtttc ctgcgattgc
     6421 aagcgtgcct gcagaatttg gcggccttcg cgccatcggc acaatgattt tcaagaatcc
     6481 ttctgagaat ccctacatct tctctttcga gttgttgagc ttcctggttc ttgcgggaat
     6541 cattgccgct gtcagcatag caagagggag agagcatcac agacacgggc cgggaaaacc
     6601 acaatgagcg ttttttctct ccagacatgt ttgattttat catcgctttt gattctgatt
     6661 ggttgctacg gcatcatcat tcgcagaagt ttgcttctga tttttttgtc tgttgagatc
     6721 gttctcaacg gcgtgcacgt tgcactggtt gcttttaact acttccgctg gtcgggattg
     6781 gaggcaggtc actacattta tatgctttcg atcggcgtcg ccgctgtgga agcggcagtt
     6841 ggtctttcta tgatcattct cattttcaaa aactcaggta cgattttgtc agacgcactc
     6901 acgaaactgg gcgaagacaa agaggaaaac aaatgaacgc ggcattgccg tggattccac
     6961 tccttcctgc tctgggcgcg gggttatgcg ccataggcgg caggaggctg ggccttcgtg
     7021 gatcctcatt cattgtagta accgcttcgc ttgtctctct tgtgttgtgc tgcctcatcg
     7081 cttttcaggg tgtcatcgcg ggcaatatct ttcaggaagg aaagtccgaa gtatttaatg
     7141 cgggcccatt ccttcagttt tcggaggaat ggtctgcgct actgggcctt cgcatggatc
     7201 tgatgtctgg cctgatgagt ctccttgtaa cgttattcgg cactctgatc ctgatgttct
     7261 ccattgatta catgagagaa gatgattcgc ctgttcgcta tttcgcaagt ttgtctttgt
     7321 ttatctctgc catgttgatc ctggttctgt ctgagaatct ggttcttctg ttcctgggat
     7381 gggaaggggt tggcctttgc agctacttgc tgatcggaca tgaatggaaa aaatcaggcg
     7441 tgcctctggc cgcgtatcgc gcatttttcg taaaccgaat tggagatctc ttttttatcc
     7501 tgggagtttt tcttctactt aagactctgg ggaccgtttc attttccgga atgtctcaga
     7561 agttcgaaac catgtccggg tcactggatc cgttggcgcg aagagatttg ttgatggcga
     7621 gtttcctgct tctgggtggc gcgttcggta agtcggcaca ggttcctctt cacgtctggc
     7681 ttcccgatgc aatggctggt cccacaccgg tcagtgctct gattcacgcg gccacgatgg
     7741 tgacggctgg cgtgtttctg gtggctcgtt tgaactggct ctacactgcg attccggaag
     7801 cgcgcggatt acttctcatc tgcggctcac tcacattgct tgtgggcgcg gtactcgcat
     7861 gctttcaaag cgacatcaag aaagttctgg cctattccac gctttcgcat ctggggttga
     7921 tgttcattgc tctggcggcg ggttctgttt ccgcgggtct gtttcatttg ttcggccacg
     7981 cttctttcaa ggcgatttct tttctggccg cgggaagtat catccttgcg gcacatcatg
     8041 aacaggatct gcataaactg cgcggatctc tgaaacatct ccctgttact cgcgttgcgc
     8101 tctggactgg agcaatcggc ggtgcggggc tggttccatt tctcgcctct ggttttttct
     8161 ccaaagaggc tgtgctgcat tcgatttcag aagcaagttt cggggtcttc ggactcacca
     8221 tctccggcgg catgttctac tggtttgttt ttggcgcaga acttctgggc gtcgtgtatt
     8281 tgtttcgcat gcttgcgctg cttgaaagca aaccagaatc ccacgaatcg catgcatcac
     8341 acgcaataga ggagaaggga gtcttcattc gcgcagttct cgccatcctg gttcttgtcg
     8401 cgattgcatt tggcgttgca tcttctcctt ttgtgagtgg gttttcgcac ttcctttttg
     8461 ataaggcaga gcatgtatcg atcgtagaca tttgcctggc atctggaagt tccatcctgg
     8521 ttgcgatcat cgtatttgta gtgtttcgtg cagacgcagc cgaagctgta gtgggcacag
     8581 gcgcgcgaag cgggctcatc aggaatttgt tctattttga taagatttac tctgcagttc
     8641 tactcgcgcc tctgggagca ataggatctg ccacggctcg cttcattgaa gtgccgattt
     8701 ttcaaggtgc acttcgcggt ggcgccgccg tgagtcggac tgtttcttca gcattttccc
     8761 gcatgcagaa agggctgata agccagtatg cggtgatcat gattgtatct gcggcgattg
     8821 tcatgctgct tgcattgttg gcataaaact atggaattac ttacgatcat cgcttttccg
     8881 gtcctggctt ttgtggtgca ctcttttctt ccaaaacaga gaggagcgtt tctggtttcc
     8941 actttgttct attccgccct tctcgtggtg cagtcccttc gcgcgttcat ttctcctgtc
     9001 tatctcaatg cagggggagt acgcctctgg ggggattttg cacttgttcc gtctttctat
     9061 ctgacaggac tcaacgcccc tcttcttttg ttgattggcc tggtccttcc tttcgtcgcc
     9121 cttctgcttc gttcggttca cgcggaggac aagcgctaca ctctttctgc ctgggtattt
     9181 ctgctgggtc ttgctgggac attcatctct caatctctga ttctgtttta tgtgttcttt
     9241 gaaacttctc tgatcggcgc gtatttctgg atcgggcttt ttggtagatc cagtgtgtcc
     9301 ggttctcctg gcggttggtc tggtctgact cgattctttc tattcactct ggtgggtagt
     9361 ctctccatgc tcgcatcgat tgcagctctt tcctcaggcg gaaaggacgt gcgttttaca
     9421 gatgtgccga ttgtgctcgg tacattctct gattcaggtc gcttctggat ttttgctgga
     9481 ttcgctctcg cttttgccat caagctaccg ttattcggct ttcatggatg gctgcgcgaa
     9541 acctacgcaa tggcgccgcc agctgcgcga gcgctcttgt ccggcatcat gtccaagctc
     9601 ggtgcgtacg gttttctcct tctcgcagtt tactttggga tagagtttcg actgcattcg
     9661 aagttgatca tcgcattgtg tgccgctgga gttttgtacg gtggactggt gagtctatcg
     9721 cagaaacgat ttctggatgt tgttgtatat tcttctcttg cgcacctgaa cttgatcgtg
     9781 atggggcttg ctgcgatgag tggttctgtt tcagatacga ccatcgtatc tgcctgcgtg
     9841 ttgcagatgt tcaatcatgg attgatcatg gccgcgatcc ttgtatacga tgcgcgtatg
     9901 ggaagcccgg atgaaagtca gtcaggaatg agggagcatg ccgggattct gtctgcgtta
     9961 ttgcttcttt ctttgtttgc cgcggtaagt ttgccgggtc tgtcaagctt tccccccgag
    10021 tttgctattc tgtatgccgc gttcaagact tctatatgga ttgctctggt cgcaggcctc
    10081 ggccttttga tttctgccgc ggctctcatt cgcagctatc acagaattta cgcaggtaat
    10141 tttgcaggaa gcgcgaatgt ggcatcgagt ggagaagttt ccaggatgga ggctgtcatc
    10201 ggtgtcatct tcggactggt ctggattggc ctggggattc ttccagggct gatcatgtcg
    10261 cccgtggcgg cagcactcgg ggtgatcaag ttatgaatgc atatttggtt tcgcagcttc
    10321 cattgattgc tggcattttt gc
//
LOCUS       DAORFF010000048        10269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_2990_length_10269_cov_3.108674, whole genome shotgun sequence.
ACCESSION   DAORFF010000048 DAORFF010000000
VERSION     DAORFF010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10269
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2990_length_10269_cov_3.108674"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="PKK76_03295"
     CDS             <1..947
                     /locus_tag="PKK76_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67520.1"
                     /translation="FFATGEELRRRISDQPCDLVILESMGNPAVVELIDELSSRKIPS
                     IVVSEECTERLVVECLKAGALDFVSKRNIKLGLLLDVVRRALLEADRWMDTRDLYSRL
                     PVRPEFIRMNQSLRTSISKEQAEVRRMRIKTGQDLGGTYEEGKSYNLIFVFLQLHFPE
                     TLARAGDDKSSKVVNEALDKIVSVPARYGGEVWVRRRDGGIFVFPAAQALAALLCAFE
                     VRGLISMLNVTTENIYENIKANLALTSGMTVYRRNVGDVHSDALNLAAHLAIQYPRSN
                     AILMPSSLVEQLSERARKYFFKGETFEGKEISVYERVL"
     gene            complement(929..1570)
                     /locus_tag="PKK76_03300"
     CDS             complement(929..1570)
                     /locus_tag="PKK76_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67521.1"
                     /translation="MSEKQTINLRVLDEKTKGVLRTTFDDLERQLKIDSLGLTLFYVV
                     MELVGNAVKANLKRVFFRTHSYNLSDPDDYRIHIDEFKAYYNAVSEEEYTRAFDDLGL
                     KVTIDIDLDHRRLLIFVENTATLHPEEEKRIRAKLASTREMKDILEFSVQFADETEGK
                     GLGLAMIVLLIRDLGFDPQYFRVYKTEDKTVARLEFPLSADYTPIRLRQSTLS"
     gene            complement(1567..2940)
                     /locus_tag="PKK76_03305"
     CDS             complement(1567..2940)
                     /locus_tag="PKK76_03305"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNL67522.1"
                     /translation="MTPEQLHQIEKELQQAAREALGEVGRADGEICCGYENKARIIFE
                     GNEFSLGAVNEERTFGVRVIDQGRMGFSSTNQPDHLLSTARVACEIARLSPASEFHCI
                     PDGTPHNVVSLIDSKLMETDSRDAGNLLEILVKAASSDKRVTIDRAEIALSTRIQSVL
                     NSKGVYSSYSITTAEYYIMGMARDGEEVTSFDYDGGVSAQRGDLESQIETTGLEFRSS
                     VIGSLHPIKGKSTRGAVLLHPRAVSDLFGSAVFFNTNARMHQDLTSSWRDKLNQSVAH
                     PSLMVTEHPLARDRAEGFMPFDREGVPASDHAIIDGGRLNFVAHNSFTARRGRSAPTG
                     NASGSPKSMPGIGFSNTSFGLKSGSVPIQTESEWLDDLKHALLLVRFSGNDDPVSGEF
                     SGVAKNSSWIEHGALRPASEVMISGNVFDLLKNIAGGTAQVFPVFGGSHAPYILVEGV
                     SITAGNE"
     gene            complement(2937..4322)
                     /locus_tag="PKK76_03310"
     CDS             complement(2937..4322)
                     /locus_tag="PKK76_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNL67523.1"
                     /translation="MHPDQLRKLISLSDCFAEVRYHKKKSRTISVRKGEVVEMSLRQL
                     DGVSIRVLKNGTFGFASTSDMTDEGLARALKSAMEMARELQTRRLHPVTLAQANLARG
                     TFATDGYQTLDQMPVEEKLGIVRKCEEDLRKSSHIQGAQCQYAEIFEEKHIVTTDGAD
                     CIVQIVRPELRFSAFAEEGGKMTSGFDSVGATGGWKCLFENRSIESYIETAAKSAVDL
                     LGAPVAEGGKHTVILSPAMVGLLSHEAIGHTVEADFVKSGSVAQGKIGHMVASELVTL
                     CDSGLSEFKDHAGGMLPVDDEGVVTGNTTIIDKGRLVSYLHNRQSAAEFGAAPTGNAR
                     AWEYSDEPLIRMRNTYVAPGSSELDHMIGEIKEGFYIDGPEGGQADATGEFMFGASRV
                     RKIKNGKLEGMVQKVSVSGNAFEVLSSVDAVSRDFQWDLGSGHCGKGQPAKVDAGGPF
                     IRCKVLIGGEQ"
     gene            complement(4425..4670)
                     /locus_tag="PKK76_03315"
     CDS             complement(4425..4670)
                     /locus_tag="PKK76_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67524.1"
                     /translation="MNNAENTAIGLVNAGIGALKAGEETLNQAVANIQKNFQEAVANA
                     EKTFEGFKAKGAADFSDPAMKTREFVATAVRTVAGDK"
     gene            complement(4809..5036)
                     /locus_tag="PKK76_03320"
     CDS             complement(4809..5036)
                     /locus_tag="PKK76_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67525.1"
                     /translation="MLSSVAAGGMVLHGALRGWILTLFDFAIEGMLLAAFVFGILTIL
                     RRRGAPVGPGVLATCLPPALYLAWILVTGES"
     gene            complement(5047..5493)
                     /locus_tag="PKK76_03325"
     CDS             complement(5047..5493)
                     /locus_tag="PKK76_03325"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HNL67526.1"
                     /translation="MREELRPMMEDEGIWDVDMEVRVPGQPVNRGKGVSTHRMACGGE
                     WLIVDFKHENGFEGHAMYGFDSLKKKYTGIWIDTMRTFLVTLEGEFDRERNHLVLHGS
                     FERPEGPYKWREVTERPDKDTRIFRHFSPGPDGEFEMIHAVYKRRP"
     gene            complement(5490..6827)
                     /locus_tag="PKK76_03330"
     CDS             complement(5490..6827)
                     /locus_tag="PKK76_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNL67527.1"
                     /translation="MMKPQTVPPGPRGFQVFTGSMHFARNPLGYMQYVVKKYGDAVLL
                     TAPNGGSFYLFNHPAQIEEILVKKNNYFKKDIFTRRLSEILGRGLLTSDGEHWLRQRR
                     LIQPVFSRERVQSYSGFVLDYAKECAEKWENGSRIEIHHAMMNLTLRIIVRAIFQIEA
                     EHFSERVGAALEAVMQHQAFRLKFPAPAFLTTTRLLSINRRYTRSLGELDQIISEIQK
                     EAMSQNEEAHNSLLEALASAKDESGSMDEKQIRDELMTILLAGHETTANALTWTFILL
                     SQNPACYGRLIQEVAALSSIDFQSLYSLPYAEAVIKESMRMFPPAWSIGREVVEECEI
                     GGFRLTTGTQVGMMQFITHRDSRFFENPDEFQPERWLDGKEKNLPRFAFFPFGGGQRM
                     CVGSNLAMMEAVLCLAQITKRVKLTLDPSSDVRPFASITLRPRGPVHMKVEQL"
     gene            complement(6824..8023)
                     /locus_tag="PKK76_03335"
     CDS             complement(6824..8023)
                     /locus_tag="PKK76_03335"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNL67528.1"
                     /translation="MFTNFFYRLRKAGLRCGTGELLDLLRALDGYADLEIPLTPERFY
                     FISRACLAKDEKYFDAFDRTFAATFQESLESEDAFRKILEQWLREKRERSQAHSGPEY
                     DTEQLWKELEDRLKNQKERHDGGSKWIGTGGVSPFGHSGENPAGLRIGGEGMRKSAMD
                     VLNSGRYREYSEDEKLGVRGFRIALRKLRDLRREGRPEFDMPETIRETSNSGGDPTAV
                     FRRSRKNRMTLVLLTDVGGSMSPYSGLVSRLFTAASRLQHFREFKHYYFHNAVYDHLF
                     LDAAFRRPLPLDDFYKKFDRETRILFAGDACMNPYELFDKRHAYFEYYYRARMKEDNP
                     REKDTLSAYERLQELRGKFPHIAWMNPEPARAWWHETISAISDVIPMFPFTIAGLQGA
                     VRELMHK"
     gene            complement(8031..8825)
                     /locus_tag="PKK76_03340"
     CDS             complement(8031..8825)
                     /locus_tag="PKK76_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002749959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNL67529.1"
                     /translation="MSSLNYVVPPALEEAVHVSEITGRPLLLKGEPGTGKTLLAESLA
                     QSRGLGIFRWHVKSISQAKDGLYFYDALSRLNDSRFAENSEKIKNIENYIQLGALGQA
                     FESESPCIVLIDEIDKADAEFPNDLLLELDQMEFSITETGRRVQAKRRPLAIITSNNE
                     KELPDAFLRRCIFHYIDFPSSDFMKVIVRSHYPDIDHKLLESSLEIFYKLRALGDLKK
                     KPSTSELIDWMRILIHEGAALSDGKIPWVGALIKNEEDLREVKSRF"
     gene            complement(8818..9432)
                     /locus_tag="PKK76_03345"
     CDS             complement(8818..9432)
                     /locus_tag="PKK76_03345"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNL67530.1"
                     /translation="MALALQKNLAVSICPQSDNPEGNKFQGQLREVGADAMQLFVAEP
                     LTLSPGDPVVVEFKIEKNQFSFEAKTQSMTGSLLSLSKPAAIKRSRLREGHRLAVDMK
                     MAYTPWTESGRFETTLVDLSEMGVRMIGRKQLKKGMLVSIDFYVKEDKIRVLCQGQVA
                     WWRATPDNDQLFETGVEFTTISNETRKRLTKFILRKNGGEEKGE"
     gene            9519..>10269
                     /locus_tag="PKK76_03350"
     CDS             9519..>10269
                     /locus_tag="PKK76_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNL67531.1"
                     /translation="MKLWSPSDSRMEGSRMREFQKAASQKAGRTLASYEDLHSWSVKE
                     PSAFWELIWNYTGIIHSQPYVSVVDDPEKMPGARWFTGARLNFAQNLLRFRDDRTAIV
                     SAGERGVRARMTYRQLFEHTMSMADHFRRLGVKPGDRIAGFVPNIAETIVCMLAATSL
                     GAIWSSSSPDFGKDAVLDRFGQIEPVVMVTADAYEFKGRVNDCLARAEELARDISSIK
                     HIVVIPFVSENPVVSLSRDHSLYSEIVSRPGQ"
BASE COUNT         2299 a         2651 c         2547 g         2772 t
ORIGIN      
        1 atttctttgc gaccggagaa gaactcaggc gtcgtatttc ggatcaacca tgcgatctag
       61 tgattcttga gtccatggga aaccctgcag tcgtagaatt gatcgacgag ctatcttcgc
      121 gcaagatccc ttcgatcgtt gtatccgaag aatgcacgga gcggttggtc gtagaatgtc
      181 tgaaagcggg agctctagat tttgtttcca aaagaaacat taaactcggg cttcttctcg
      241 acgtagtgcg tcgagctttg ctggaagcag acagatggat ggacactcgc gatttgtact
      301 ctcgcctgcc tgttcgcccc gaatttattc ggatgaatca atcccttcgt acatccatca
      361 gcaaggagca ggcagaagtt cgcagaatgc gaatcaaaac cggccaggat ctgggtggaa
      421 cgtacgaaga aggcaagtcc tacaacctga tcttcgtgtt tttgcaactt catttccctg
      481 aaactctcgc acgcgcagga gacgacaaat caagcaaggt cgtcaatgaa gcactcgaca
      541 agattgtgtc cgttccagcg cgctacggcg gggaagtgtg ggtgaggcga cgagatggtg
      601 gaatcttcgt atttccggcg gctcaggcgc ttgcagctct tttgtgtgcg ttcgaagtgc
      661 gcggtctcat cagcatgctc aacgttacga ctgagaatat atacgaaaat atcaaagcca
      721 atcttgcact tacttccggt atgactgtat atcgcagaaa cgtgggagat gtgcacagtg
      781 acgcgctcaa tctcgccgca cacctggcca ttcaatatcc gcgtagcaat gcgattctca
      841 tgccatcttc tcttgtggag cagctcagcg aaagagcgcg caaatatttc ttcaaagggg
      901 agacgttcga aggaaaagaa atttccgttt acgaaagagt gctctgacgc aaccggatgg
      961 gcgtgtaatc cgcggagaga ggaaactcca ggcgcgccac tgtcttatct tccgttttgt
     1021 agactcgaaa gtactgcggg tcaaatccaa gatcgcgaat caaaagaaca atcattgcca
     1081 gcccgaggcc tttgccttct gtttcatccg cgaattgaac actgaattca agaatatctt
     1141 tcatttcgcg cgtgcttgcg agcttcgcgc gaatgcgttt ttcttcttcc gggtgaagtg
     1201 tagcagtgtt ttctacaaag atcaagagtc ttctgtggtc caggtcaatg tcaatggtga
     1261 ctttcagtcc aagatcgtcg aatgctcgag tgtattcttc ctctgagacg gcgttgtagt
     1321 acgctttgaa ttcatcgata tggattctgt aatcgtctgg atcactcagg ttgtagctgt
     1381 gcgtgcgaaa gaagactctc ttgaggtttg ctttgactgc gtttccaacc agttccatga
     1441 ccacgtagaa caacgtgagt ccaagactgt ctattttcag ctgacgttca agatcgtcaa
     1501 aagtagtgcg aagcactccc tttgttttct catccagtac gcggaggttg attgtttgct
     1561 tttcgctcat tcatttcctg ccgtgatgct tacaccttcc acgagaatgt agggtgcatg
     1621 gcttccgcca aataccggga aaacctgcgc agtgccgccg gcaatgttct tcagtagatc
     1681 aaatacgttt ccggatatca tcacttcgct tgcgggcctc aatgctccat gttctatcca
     1741 gcttgaattc ttggcaaccc cggaaaactc gcccgaaaca ggatcgtcat ttccgctgaa
     1801 gcgcaccagg aggagagcgt gcttgagatc gtccaaccat tccgattctg tctggatggg
     1861 cacagaaccg ctttttagtc cgaaagaagt attcgagaat ccgattcctg gcatagattt
     1921 tggacttccg gatgcatttc cagtcggagc cgatcttcct ctgcgggcgg taaaggagtt
     1981 atgtgcaacg aaattcaacc gacctccatc gatgatggcg tgatcggatg cgggcactcc
     2041 ttctctgtca aaaggcatga agccctctgc gcggtcgcgg gccagcggat gctccgtgac
     2101 catcagggag ggatgagcga ccgattgatt cagtttgtcc ctccagctgc tggttagatc
     2161 ctggtgcatg cgtgcatttg tattgaagaa gacagcgctt ccaaatagat ctgaaacggc
     2221 gcgcggatga agcaacaccg ctcctctggt tgattttcct ttgatgggat ggagactccc
     2281 tatgacagac gagcgaaatt cgagaccagt ggtttctatc tgcgattcca gatcacctct
     2341 ctgtgcggaa accccgccat cataatcaaa ggaggtgacc tcttctccgt cccgtgccat
     2401 gcccatgata taatattctg ctgttgtaat ggaataggac gaatagacgc cctttgagtt
     2461 taagacgctc tgaatgcgag tactcaatgc gatttcggct ctgtcgatcg tgactctctt
     2521 atcagaggac gcagctttca ccagaatctc cagtaaattt ccagcatcgc gggagtccgt
     2581 ttccatgagc ttcgagtcta tgagagatac aacgttatgc ggcgttccgt ctggaatgca
     2641 gtgaaattcg gaagcgggag agagtcttgc gatttcgcat gcaacgcgcg ctgtggacaa
     2701 tagatgatcg ggctggtttg tggaagagaa tcccattcgt ccctggtcga ttacgcgcac
     2761 tccgaacgta cgttcttcat tcacagcacc aagtgaaaac tcattccctt caaaaatgat
     2821 tctggccttg ttttcatacc cgcagcagat ttctccgtcc gcacgtccca cttcgccgag
     2881 cgcttctctg gctgcctgtt gtagttcttt ttctatttga tggagctgct cgggggtcat
     2941 tgttctccgc cgatcaacac tttgcagcga atgaacgggc ctcccgcgtc tactttcgca
     3001 ggctgtcctt tcccgcaatg gccgctgccc agatcccact ggaagtcgcg actgactgcg
     3061 tccacgctgg ataacacttc gaatgcattt ccggaaacag acactttttg caccatgcct
     3121 tctagttttc cattcttgat ttttcgaacg cgggacgcac cgaacatgaa ttcgcccgtt
     3181 gcatctgcct gtccgccttc cggtccgtct atatagaagc cttctttgat ttcgccaatc
     3241 atgtggtcga gttcagaaga cccgggcgca acgtaagtat ttctcattcg gatgagaggt
     3301 tcgtccgagt attcccaggc tcttgcattt cctgtgggtg cggccccgaa ttcggctgcg
     3361 ctctgtctgt tgtgcaagta gctgacgagt ctgcctttgt ctatgatcgt cgtgtttcct
     3421 gtaacgactc cttcatcgtc cacgggcagc ataccgcctg catgatcttt gaattcgctc
     3481 agaccgctgt cgcataacgt tacgagctcg gaggcaacca tatgtccgat cttaccctga
     3541 gctacgctgc cgctttttac aaagtctgct tccacagtgt gcccgattgc ctcgtgcgaa
     3601 aggagcccaa ccattgctgg agagagaatt actgtgtgct taccgccttc cgccacgggg
     3661 gctccgagta gatcgactgc gctttttgcg gcggtttcta tgtaggattc aatcgatctg
     3721 ttttcgaaca aacatttcca gccgcccgtg gcacccactg aatcaaatcc actggtcatc
     3781 tttccacctt cttctgcgaa ggcggagaag cgaagctccg ggcgaacaat ttgaacgata
     3841 caatccgcgc cgtctgtagt tacgatgtgc ttttcttcaa agatttcggc gtactggcac
     3901 tgcgcgccct ggatgtgcga tgattttcgc aagtcttctt cgcactttcg cacaatgcca
     3961 agcttttctt ctacaggcat ttgatcgaga gtctggtagc cgtctgttgc gaaagtgccc
     4021 cgcgcaagat tggcctgtgc cagagtcaca gggtgaagac gccgcgtttg aagttcacga
     4081 gccatttcca tggcagactt caatgcacgt gcgagacctt cgtctgtcat gtccgaagtg
     4141 gatgcgaatc caaacgttcc atttttgaga actcgaatgg atactccatc gagttgacgc
     4201 agactcatct ctaccacttc tcccttgcgg accgaaatgg ttctggattt ctttttgtga
     4261 taacggactt cagcgaaaca atctgagagt gaaatgagtt ttcgaagctg gtctgggtgc
     4321 acgttggtaa gaaagtgaga aggggtgcgg tgtgccaggc aaaaataaca gatcctgcag
     4381 tagaaaaaaa atcacggccg ggcggatgcc cggccaggga gcgattactt gtcgccagct
     4441 acagtgcgaa ctgcagttgc tacgaactcg cgagtcttca tggccgggtc tgagaaatcc
     4501 gcggcacctt ttgctttgaa gccttcgaat gtcttctcag cgttagccac tgcttcttga
     4561 aagttcttct gaatgttagc tacagcctgg ttcagagttt cttcccctgc cttcagtgca
     4621 ccgattccgg cgtttacgag gccaattgca gtgttttcag cgttgttcat ctttgactcc
     4681 ttcgcttaat gcgatgcacc attttttttg gtgcgccgca caagtcaagc caaaacggca
     4741 tccaggatcc agacgcgcgg ttttttttag ggcggccggc cgcgacctca gaatggccat
     4801 tttctggctc aggactcgcc tgtgaccaga atccacgcga gatagagagc aggaggcagg
     4861 caggttgcca ggacacccgg gcccacggga gctccgcgcc tgcggaggat ggtcaggatt
     4921 ccaaatacaa aggcagcgag gagcatacct tctatcgcaa agtcgaataa cgttaagatc
     4981 catccccgga gggctccgtg gaggaccata cctccggcag ctacgctcga aagcaaaatg
     5041 tcttcttcaa ggtcttctct tgtaaacggc gtggatcatt tcgaactcac cgtccgggcc
     5101 gggagaaaaa tgacgaaaga ttcgcgtgtc cttgtcaggc cgttctgtca cttctctcca
     5161 tttgtaaggg ccttccggtc tttcaaaact gccgtgcagt actagatgat tcctttctct
     5221 atcgaactct ccttccagag tcacaagaaa cgttcgcatc gtgtctatcc agatgcccgt
     5281 atatttcttc ttcagtgaat caaagccgta catcgcatgg ccttcaaatc cattttcgtg
     5341 tttgaagtct acgatcaacc attcgccgcc gcaagccatt ctatgagtgc tgactccctt
     5401 gccgcgattc accggctgac cggggacacg cacttccatg tccacgtccc agattccttc
     5461 gtcttccatc atgggcctga gttcttctct catagctgtt ccaccttcat atgaactggt
     5521 cctcttggtc tgagtgtaat cgacgcgaat ggtcgaacgt cggatgacgg gtccagagtg
     5581 agtttcactc tctttgtgat ttgcgccaga cacaatacag cttccatcat ggccaggttg
     5641 gaaccgacgc acattctctg tccgccgccg aagggaaaga aagcaaacct ggggaggttc
     5701 ttttcctttc cgtccaacca cctttctggc tgaaactcat ctggattttc gaagaaccgg
     5761 gaatcacgat gagtgatgaa ctgcatcatc cctacctgcg ttcccgtcgt taggcggaat
     5821 ccgccgattt cgcattcctc caccacttcg cgtccaatgc tccaggccgg cgggaacata
     5881 cgcatagatt ctttgattac agcttccgcg tagggcaggg aatagagcga ttgaaaatca
     5941 atcgaactca aagcggcaac ttcctgaatc aggcgcccgt agcatgcggg gttttgggaa
     6001 agtagaatga aagtccaggt caatgcattg gcagtggttt catgaccggc gagcaggatg
     6061 gtcatgagtt cgtctcggat ttgtttttcg tccatggatc cggattcatc ttttgcgctg
     6121 gccagggctt cgagaaggct gttgtgtgcc tcctcgtttt gagacatggc ttccttctgg
     6181 atttctgaaa tgatctggtc gagttctccg aggctgcgcg tataacgtct gttgatcgag
     6241 aggagtctgg tagttgtcag aaacgcgggg gccggaaact tcaaacggaa cgcctggtgc
     6301 tgcatgactg cctcgagagc cgcgcccact ctttctgaaa aatgctctgc ctcaatctgg
     6361 aagatcgcgc gaacaatgat gcgcaatgtc agattcatca ttgcatgatg aatctcaatc
     6421 ctcgatccgt tttcccactt ttccgcgcat tcttttgcgt aatccaggac aaatccgcta
     6481 taggactgaa ctctttcgcg actgaacaca ggctggatga gtcttctctg cctgagccag
     6541 tgctctccat ccgaagtaag cagtccccgc cccaggattt cgctgagtct tctcgtgaat
     6601 atgtctttct tgaaatagtt gtttttctta actaatattt cttctatctg tgctgggtgg
     6661 ttgaacaaat agaaggatcc tccgttcggt gctgtcaata acactgcgtc gccgtatttt
     6721 ttaacgacat attgcatata tcccagcggg ttgcgtgcga agtgcatgct tcccgtaaaa
     6781 acctggaatc ctctggggcc gggcggcacg gtttgtggct tcatcatttg tgcatcaact
     6841 cccgcacggc accctgcagg ccggctatag taaatggaaa catggggatg acatccgaaa
     6901 tggcggaaat agtttcgtgc caccaggctc ttgccggttc cgggttcatc caggcaatgt
     6961 gtggaaattt gccgcgcagt tcctgcaatc gttcgtatgc ggaaagtgtg tctttctcgc
     7021 gcggattgtc ttctttcatt cgagctctat aatagtattc aaaataggcg tgtctcttat
     7081 cgaacagttc gtacggattc atacatgcat cgccggcgaa aaggatgcgc gtttcgcggt
     7141 caaatttctt ataaaaatcg tctaacggca gtgggcggcg aaatgcagcg tctagaaata
     7201 gatgatcata cactgcattg tggaagtagt agtgtttgaa ttcacgaaaa tgctgcagtc
     7261 tggatgctgc agtgaatagc ctgcttacga gaccggagta gggcgacata ctgcctccaa
     7321 catctgtcaa aagtaccagg gtcattctgt tttttctgga cctgcgaaag actgctgtcg
     7381 ggtctccgcc gctgttcgat gtttcgcgaa tggtttcagg catgtcaaat tctggacggc
     7441 cttctcgtct gagatcgcgc aactttcgca gtgcgattct aaatccacgc actcccagtt
     7501 tctcatcctc gctgtattcg cgataccttc cagaattgag tacatccatt gcggattttc
     7561 tcatgccttc tccgcctatc ctgagacctg cgggattttc tccgctgtgt ccgaagggag
     7621 aaacaccacc cgtgccgatc cacttgcttc ctccgtcgtg tctctccttc tggtttttca
     7681 gtctgtcttc cagttctttc cagagttgct ctgtgtcata ttcggggccg ctgtgcgcct
     7741 gactccgttc tcgcttttct ctcagccatt gttctagaat ttttcgaaac gcatcttctg
     7801 attccagaga ctcctggaag gtggcggcaa atgtgcgatc aaatgcgtcg aaatactttt
     7861 cgtcttttgc aaggcatgcg cgcgaaataa aatagaatct ctccggtgta agtggaatct
     7921 ccagatctgc atacccgtct agagcgcgca gcaaatccag gagttcgcct gtgccgcagc
     7981 gcagtcccgc tttcctcagt ctgtaaaaaa aatttgtgaa catagcggtc ctagaacctg
     8041 cttttgactt ctcgcaaatc ctcttcgttc ttgatgagtg ccccgaccca cgggatcttt
     8101 ccgtccgaaa gagcggcccc ttcatgtatc aggattcgca tccagtctat gagctcggat
     8161 gtggacggtt tctttttcag atcgcccaat gctcttagtt tgtagaaaat ttccagagaa
     8221 gattcgagga gcttatggtc gatgtccggg taatgcgagc gaacaatgac tttcatgaag
     8281 tcagagctcg ggaagtctat gtagtgaaat atgcatctgc gcaaaaacgc atccggcaat
     8341 tctttttcgt tgttggatgt aatgatggcc agtgggcgcc tttttgcctg cactcttctt
     8401 cctgtttccg taatggaaaa ctccatctgg tcgagttcta gtagaaggtc attgggaaac
     8461 tccgcatctg ccttgtcgat ttcatctatg aggacaatgc acggtgattc gctctcaaaa
     8521 gcctgaccca gagcgcccaa ttgtatataa ttctcaatat tctttatttt ttcagaattt
     8581 tcagcaaacc tggaatcgtt gagacgagaa agcgcatcgt agaaatacag cccgtctttt
     8641 gcctgtgaaa tggactttac atgccagcga aagatgccca gcccccgaga ctgtgccaga
     8701 gattctgcaa gcaaagtctt tcccgtcccc ggttctcctt tgagtagaag cggacgcccg
     8761 gtaatttctg atacatgcac cgcttcttcg agcgcgggtg gaacaacata gtttagacta
     8821 ctcacctttt tcttctccgc cgttcttcct gaggatgaat ttagtcaggc gctttcgtgt
     8881 ttcgtttgaa atcgtagtga attcaacacc cgtctcgaat agctgatcat tgtccggagt
     8941 cgctctccac cacgctacct gaccctggca aagaactctg atcttatcct cttttacata
     9001 aaaatcgatc gagaccagca tgcctttctt taattgcttt cttccaatca ttcgcacgcc
     9061 catttcgctc aagtcgacga gagtggtttc aaatctgccg gattctgtcc acggagtgta
     9121 tgccattttc atgtcgactg caagcctgtg gccttcgcgc agtctcgagc gtttgatagc
     9181 ggcgggtttg ctgagggaca atagagaacc agtcatggac tgcgtttttg cctcaaagga
     9241 aaattgattc ttttcaattt tgaattcaac gaccactgga tccccgggag aaagagtgag
     9301 gggttcggcg acgaacagtt gcattgcatc cgcgcctact tcacgcaatt gtccctgaaa
     9361 tttgttccct tccggattgt cggactgggg gcagatgctg acggccaggt tcttttgaag
     9421 tgccagtgcc atggacagat tcagcggcca caccgcgagc ccgtcaagga aagcgcgaat
     9481 tcacttgaag aatctcacgg cgatcacaga caagcagaat gaagctctgg tccccctcgg
     9541 attcgcgcat ggaaggctct agaatgcgcg agtttcaaaa agcggcctca cagaaagcag
     9601 gacgcacgct tgcaagctat gaagatctcc attcctggtc tgtgaaagaa ccatctgctt
     9661 tctgggaatt gatctggaat tacactggca tcatccattc acaaccgtac gtttctgttg
     9721 tagatgatcc cgaaaaaatg ccaggagctc gctggtttac aggagcccga ttgaatttcg
     9781 cgcagaatct acttcgcttc cgggatgatc gcacagcgat tgtttctgcg ggggaacgtg
     9841 gagtccgggc acgcatgacc tacagacaac tctttgaaca caccatgtcc atggcggatc
     9901 attttcgtcg tctgggagtg aagcccggag accgaatcgc cgggtttgtt ccaaatatcg
     9961 cagagaccat tgtttgcatg ctggccgcaa cttctctggg agcgatctgg agttcgagca
    10021 gcccggattt tggaaaagac gccgtgctcg atcgctttgg tcagattgaa cccgttgtga
    10081 tggtcacagc ggatgcttac gaattcaagg gacgagtgaa tgactgtctg gcccgcgcgg
    10141 aagagctggc ccgtgatatt tcttctataa aacatatagt tgtgatccct tttgtttctg
    10201 agaatccggt cgttagcctt tcgcgcgatc actcccttta ttctgaaatc gtaagcaggc
    10261 cgggccagg
//
LOCUS       DAORFF010000049        10242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3008_length_10242_cov_3.439285, whole genome shotgun sequence.
ACCESSION   DAORFF010000049 DAORFF010000000
VERSION     DAORFF010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10242
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3008_length_10242_cov_3.439285"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1942
                     /locus_tag="PKK76_03355"
     CDS             <1..1942
                     /locus_tag="PKK76_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNL67532.1"
                     /translation="ERARSEGKTLSLITVNLARDPAADLRTSLRAFESLWNGTTVLGA
                     MIREDRLSGLDSTDLSVPCSFLILTPGDPTEIAEINSLSPDHLQVIYSAAPVPTNLPA
                     EVHSRVERIDHPKKTEETHLRVSVAVIDRLINLAGEAVGARNEFMQKLRKDPVLFRAG
                     GRINSLITRLQEEVMRVRLQPLRVFFQRLPRLVRDTAAATQKEVRLVILGDSVELDKR
                     LLDTIADPLVHMIRNAIDHGIESSAERLQKGKPAAGTLEVKAQLMGGNVVIQIEDDGR
                     GLNHRAILQAAEAHGLVDSVRAASLSPDEIAEFIFHSGLSTKSKVTETSGRGVGMDVV
                     RTSFESAGGSIVVRSQPGAGTQFIASLPQTLSIITCLIVRAGSQRFAIPQMNAVELVR
                     FDPSAVAQMSSATVYRLRSRLLPIIDLKQLFTDEPSEKIGFLVVVRSERGLYGLTIEG
                     LDNPEEILVKPLGRQLQEIGLYAGAAVLADSSAVPILDTGGIARSVNLSSSGIDSVAQ
                     SAEDAITDAAFAGLVFQYGGSEFALPVESFPRVEKRTRYQEESSLGKEVVHYTSGPIP
                     LIRLESLLPVPASDGAFLVIFTRGGRTAAIVADEALRVGNDLPPVELGLDPGSPILGR
                     CVVDSRTIVVLSPEVLLNGGAS"
     gene            1939..2358
                     /locus_tag="PKK76_03360"
     CDS             1939..2358
                     /locus_tag="PKK76_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNL67533.1"
                     /translation="MSIHRQYLVFCMRGVRYGVALDSCHEVNLRSRVYPVPRARPHIS
                     GIMNLRGEVVTVIDLPSMFSPSKGEASSGAVIRLKAGEHPIAIRVDAVEDILTTENAL
                     EPPPPHLAEEHLRLIAGVIQTENGIVQIIDPQGILRV"
     gene            2370..4037
                     /locus_tag="PKK76_03365"
     CDS             2370..4037
                     /locus_tag="PKK76_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HNL67534.1"
                     /translation="MKAQGMKDAEKKQMNVTLTGKERIMDDGDILVSMTDLKSRVVYA
                     NKAFLKIAGYTQEELVGKPHNVVRHPLMPRSAFKDFWQTLQAGKPWNGLVVNRATNGD
                     HYWVDANVAPRFENGNLVGYMSVRRKPQRDQIEKAARLYRSVLEGKATVPSSLRKRMS
                     LKAKFWLFLVFVTVVVMGLGLDAAFDLLHGKVAVGLSVVFSVLTLAVGAGLSNSVLRP
                     IKEAADYARLIATGDLSVRIPHDRNDEVGDLFQALLSMLVNTAAVIGQIKESVESLDS
                     AAGNLTSASTSLSAGLDKVAQQSETFAGIASAATQMNTNLQSVTSSTEEMSISIQEVA
                     RKSSDSATVAEKAQITSREMNSLVKELGVNARDIGKVIESISEIASQTNLLALNAAIE
                     AAGAGDAGKGFAVVASEVKELARQSAESSEEIKDKIGAIQGSTENVIAAIGSITSVIS
                     QINEIAGSIASAVEEQAITTREIASNINQTAQASNDVTASINNVSTAVLSGAQESASF
                     TRLAVDLKDLAGNLNNLVTRFKLSSNGAGTGTIQTMQGSQEMTPEFV"
     gene            4037..5062
                     /gene="cheB"
                     /locus_tag="PKK76_03370"
     CDS             4037..5062
                     /gene="cheB"
                     /locus_tag="PKK76_03370"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HNL67535.1"
                     /translation="MQPRISVLIVDDSAAYRNALRDVLASDPGLDVVGQASNGRLALP
                     RIRYHKPAVVILDQEMPEMDGLSTLAAIREEWPAVHVIMFSSRTQRGAETTVKALQLG
                     AADFLAKPAEKDTDIPLYLRSHLLPRIHALGQRFSLSYSSHMRSNVTGDFKVAALGIS
                     TGGPPVLKTILNKLNRGLHASVLIVQHMPPVFTAQLARSLSETSGIPVKEAEDAEPLA
                     PGFAYIAPGGRHLEVSSDGSQLRITDGDPELSCRPSVNVLFRSVARAFGPQALGLILT
                     GMGEDGYSGIQEIRARGGKIIAQSQDSCLIFGMPARPVREGLVDAVLSPDKIAERLNE
                     LFAGVRT"
     gene            5059..5922
                     /locus_tag="PKK76_03375"
     CDS             5059..5922
                     /locus_tag="PKK76_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNL67536.1"
                     /translation="MSNSPPNLTRIKTVIRAKTGVIIDDKNEYLIGSRLGILLEENNL
                     SSFDELADVLAGPQDSPIFKKFLERITTHETRFFRDESTFAALVEQMIPEWMERNRSF
                     GGASRPLNIWCAAGSTGQEAYSIAMLIHSKFPQIFPSLNLVSTDISETCVERASAGFF
                     TDYEMNRGVSDELRAKFFVRVSDKGYNISSEIRSKVEFKQQNLLVTPYAGKFDIVFCR
                     NVLIYFDLAVRKQILEGFQRVILPDGFLVLGATESPTGLLDSYVVRQFGLTRYYEINS
                     TNITFAPAQMR"
     gene            5926..6282
                     /locus_tag="PKK76_03380"
     CDS             5926..6282
                     /locus_tag="PKK76_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL67537.1"
                     /translation="MKKVLAVDDSQAMLKMVQQTLQIGGYEVLVASDGKEALEKFRGA
                     GVELLITDINMPVMDGITLIKEIRAADKEVPILTLTTESEEPMKQSGFAAGANGWIVK
                     PFRPAQFLDIVRQILS"
     gene            6282..7466
                     /locus_tag="PKK76_03385"
     CDS             6282..7466
                     /locus_tag="PKK76_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL67538.1"
                     /translation="MTTPVVLFAGPGLDALAASIRENQYTVLTAESGPQALDLLGAVR
                     VDFFFVAAGSSAAPYVSVLRKKSEAKLILVGNDTSSVPCDRVSSAHAVEEALALLHRL
                     TADRANKELMEQAEGRKVDAEQRRLEWLTWKQQMADRLHMQYSAALVRNIRHSMTQGL
                     GAGALITQVDILPLLPRNPSGDYIVPESSVTFLGEAAEALRHWLSSMEMFVEAMERMY
                     PAETIPEADLSILVSEATQRVDHLRRIKRQTVRSALPTPAGPIRMSRIGLSLAIRELL
                     TNAFKYSPDASRVDIAGFSSQGGYSICILNDLVDLPAGITGIPPERENEIFEPFRRLH
                     NVYDDRFAMEEFPMGTGLTVVRQAIQQAGGSVSLFEIRDHTREDTKKRRVMAEILLPL
                     DI"
     gene            complement(7446..7778)
                     /locus_tag="PKK76_03390"
     CDS             complement(7446..7778)
                     /locus_tag="PKK76_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67539.1"
                     /translation="MEILDFTVLSRYSPNALTTMFGVFESDTVTGLPTITHLMQSGAN
                     PTELARLCHRTRSGCLVMGASRLASVLFDIEKSPGSNEKSPNAEELEDAAKETIAALR
                     EYLDIQGK"
     gene            complement(7765..8487)
                     /locus_tag="PKK76_03395"
     CDS             complement(7765..8487)
                     /locus_tag="PKK76_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL67540.1"
                     /translation="MRAICSCALHETSICSLCFEVTDTGIGIEEDRLDKIFNPFAQAD
                     SSTTRRYGGSGLGLSISRELAVMMGGTLTARANHPQGTTFRFESKVIIASPAGRQPLK
                     HPDHQRIENGPRILVADDHELNRMCISLMLRERGLACDLATNGQEALEAARTGQYKII
                     FMDCQMPGLDGYAATQRIRQENLNYSPAIIALTANALEGEKQRCFEAGMDDYIAKPID
                     FERMFELIGRYSGEPEDKNGNS"
     gene            complement(8488..9747)
                     /locus_tag="PKK76_03400"
     CDS             complement(8488..9747)
                     /locus_tag="PKK76_03400"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNL67541.1"
                     /translation="MAVIHENVTARVTTQIRQQELESLFIDSTRIAKLGGWRLDRKSG
                     LIICTETLLELLDLSPRARLTWPVIKSMISRGSAQVLIGAVHRAFKRGAGFDVQFQIE
                     TATGKWIWVRCAGEARRRFSDITGLSGILQDVSASVRSDIALDMERKRLELAMQISRQ
                     ALFEIDIPSGDIFWDARAFEIHGQNPSAFTPNLRTLDSLLIPEDQGLTDRVQAFFESE
                     RRIWHEQYRIKTPDDGIHYIETLGTILRTDRPRIIGIARDITARREIQLELVQAREAA
                     EAATRAKSRFLANMSHDIRTPMTGILGFIKLLQNSGLNQDQQSYADMAASAAGSLRTI
                     LDDILDFSRIESGMLRLEKIPVNVADLLSECTGLFRLSAMEKGVELVYDLAPETPAFI
                     LGDPVRLKQILNNLIGNAVKFTEKGRI"
     gene            complement(9798..>10242)
                     /locus_tag="PKK76_03405"
     CDS             complement(9798..>10242)
                     /locus_tag="PKK76_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67542.1"
                     /translation="PTETMPPHTGVRVKRKDGELLEANIIVTRLTSESGTHYLATVRP
                     CEEERPNKSNSAEFAQHVLDAIDAHVAIMDSSGTIVAVNSAWRRFAEQNDGRDVTEGA
                     NYLLVCDRASADDRAVEEIASGIRKLLQNGGAHFDMSTRAIPGEG"
BASE COUNT         2382 a         2545 c         2740 g         2575 t
ORIGIN      
        1 ggagcgagcc agatcggaag ggaagactct tagtcttata acagtaaacc tcgcgcgtga
       61 tccggccgcg gatcttcgca cttcactgcg tgcatttgaa tcgctctgga atggaacgac
      121 tgtcctggga gccatgatca gagaagatcg cctatcgggt ctggattcaa cggatctatc
      181 tgttccatgt tcatttctca ttctgactcc gggcgatcca actgaaatag cggagatcaa
      241 ctctctgagc ccggatcacc tacaggtgat ctattcggct gccccggttc ccaccaatct
      301 acctgcggaa gttcattccc gcgtggaacg tatcgaccat ccgaagaaga cggaagagac
      361 acatctgcgt gtttccgtcg ctgtcattga tcgactcatc aatctggcgg gcgaagctgt
      421 gggcgcgcgc aatgagttta tgcagaaact ccgcaaggat cctgtacttt tcagagctgg
      481 cggaagaatc aattcattga tcacgcgact tcaggaagaa gtcatgcgag tccgtctgca
      541 accgcttcgt gttttcttcc agagacttcc gcgtcttgtg agagataccg cggccgcgac
      601 gcaaaaggaa gtgagactcg tcatcctggg ggattcagta gaactggata aacgtcttct
      661 ggacacgatt gctgatccgc ttgtgcatat gattcgcaat gcgatcgatc atgggattga
      721 atcgagtgcg gaacgtttac agaaggggaa gccggcagct ggtactcttg aagtcaaagc
      781 gcagctgatg ggcggcaacg ttgtcattca aattgaagac gacggcaggg gtctcaatca
      841 ccgcgcgatc ctgcaggcag cggaagcgca tggacttgtg gattctgtcc gtgctgcatc
      901 tctttcgcca gatgaaattg ctgaatttat ttttcattcc ggtctgagta ctaaatcaaa
      961 ggtaacagaa acatccggtc gaggcgtggg catggatgtg gtgcgcacga gttttgaatc
     1021 agcaggtggg tcgattgtgg ttcgcagtca gcctggtgct ggaactcagt tcatcgcctc
     1081 tttgccgcaa actctttcta tcatcacttg tttgatcgtt cgcgcaggat cgcagaggtt
     1141 tgccatcccg cagatgaatg cagtcgagct tgtgcgattt gatccgtccg cagtcgcgca
     1201 aatgagcagt gcaaccgtat atcgtcttcg ttctcggctc ttgccaatca ttgatctaaa
     1261 acaactattt acagacgagc catctgagaa gattggattt ctcgtcgtgg ttcgctccga
     1321 gcgcggactg tacggtttga ccatcgaggg cctggataat ccggaagaaa ttctcgtgaa
     1381 accgctgggg cgtcagcttc aggaaatcgg actctatgcc ggtgcggcgg tcctcgcaga
     1441 cagcagtgca gtgccgattc tagacacggg tggaattgcg cgttcggtga atctatcgag
     1501 ttccggcatt gattccgttg cgcaatccgc ggaagacgca attacggacg ctgcctttgc
     1561 cggactggtc tttcaatatg gcggatccga attcgctctt ccggttgaat cgtttccgag
     1621 agtggaaaag cgcactcgtt atcaagaaga gagcagcctc ggaaaggaag tggtccacta
     1681 caccagtggt ccgattccac tcattcgcct ggaaagtttg cttcccgttc cagcaagcga
     1741 tggtgccttc cttgtgattt tcactcgagg cggacgcacg gcagcgatcg ttgcggacga
     1801 agcgttgcga gttggaaatg atctgcctcc tgttgaactc ggtctcgatc caggttcccc
     1861 tatccttggc cgatgcgttg tcgattcaag aaccattgta gtgctttcgc ctgaagtgct
     1921 tctgaacgga ggcgcatcat gagcattcat cgtcaatacc tggtgttttg tatgcgagga
     1981 gtacgctacg gcgtggctct cgattcctgt catgaagtga atcttcgttc tcgcgtttat
     2041 ccagtaccgc gtgcgcgccc tcacattagc ggcatcatga atctgcgcgg agaagtagtt
     2101 acagtcatcg atctgccgtc aatgttttcc ccttcgaaag gggaggcatc gagcggcgcc
     2161 gtgattcgac tgaaggcagg cgagcatccg atcgcgattc gcgtcgatgc ggtggaagat
     2221 atactgacaa cagagaacgc tctagaaccg ccaccacctc atctggcgga ggagcacctg
     2281 cggctgatcg ccggtgtgat ccagactgaa aatggaatcg tacaaatcat tgatccacaa
     2341 ggaattctta gagtttagtc ggaggaagca tgaaggcaca agggatgaaa gacgctgaaa
     2401 agaagcaaat gaatgtaacg ctcacaggaa aagagcgcat catggatgac ggagacattc
     2461 tagtgtctat gacagatttg aagtcgagag tagtgtatgc gaacaaagct tttttgaaga
     2521 tcgcaggcta cacgcaggag gagctggtag gaaagccgca taacgttgtg cgtcatcctt
     2581 tgatgccacg ttccgctttt aaagatttct ggcaaacact ccaggctgga aagccatgga
     2641 atggcctcgt cgtgaataga gccacgaacg gggatcatta ctgggtggat gcgaacgtgg
     2701 caccgcgatt cgagaatgga aatctggtcg gttatatgtc cgttcgccgc aaaccgcaac
     2761 gggatcagat tgaaaaggcc gcgcgcctgt accgcagcgt tctggaggga aaggcaaccg
     2821 tcccttcttc tctgcgcaaa cgaatgtctt tgaaagcgaa gttctggtta ttccttgtat
     2881 tcgtgactgt cgtagtgatg ggccttggcc tggatgcagc attcgatttg cttcacggta
     2941 aggttgctgt cggcctttcc gtagtctttt ccgtattgac tcttgcggtc ggcgccgggc
     3001 tgtcaaattc tgttctccgc ccgattaagg aagctgcgga ttatgctaga ttgatcgcta
     3061 ctggagattt gagcgtgcga attccgcatg atcgcaatga cgaagtaggg gatctattcc
     3121 aggctctgct cagcatgctc gtgaacacgg ctgccgtgat tggtcaaatc aaggagagcg
     3181 tggagtcact ggattctgcc gccggaaatc taacttccgc cagtacgagt ctctctgccg
     3241 gattggataa agtggcgcag cagtctgaga ctttcgcagg tatcgcgagt gccgcgactc
     3301 aaatgaatac gaatcttcaa tctgtgactt cttcgacaga agagatgtcc atttccattc
     3361 aggaagtggc gaggaagtcc tccgactctg caactgtcgc agaaaaggca caaatcactt
     3421 cgcgcgaaat gaacagcctg gtcaaagagc ttggagtcaa tgcacgcgat atcgggaagg
     3481 taatcgaaag tatttccgag atcgcatccc agaccaatct tctcgcactc aatgccgcta
     3541 tagaagcggc cggtgcgggc gacgcaggca aaggattcgc agtggttgcc tcagaagtca
     3601 aagagctggc caggcaatcg gctgaatctt cggaagaaat caaagacaag attggcgcga
     3661 ttcagggaag cacagagaac gtgatcgccg caatcggaag cattacaagc gtcatttcgc
     3721 aaatcaacga aattgccgga tcgatcgcaa gcgcagtgga agagcaggcc atcactacgc
     3781 gcgagatcgc atccaacatc aaccagacag cccaggcatc caatgacgta accgcgagta
     3841 tcaacaatgt ttctacggca gttctctccg gcgcacagga atctgccagc ttcactcgtc
     3901 tggctgtgga tctaaaagat ctggcgggaa acttgaacaa tctagtgact cggttcaagt
     3961 tgtcgtctaa cggtgcggga acaggcacta tccaaactat gcagggatca caagaaatga
     4021 ctccagagtt cgtttgatgc aacctcgcat ttccgttttg atcgtggacg actctgcagc
     4081 gtatcgaaac gcgctgcgag atgttctggc cagcgatccc ggattggatg tggtaggcca
     4141 ggcctccaac ggtcgcctgg ctttgccgcg catccgttat cataagccag ccgtggtaat
     4201 tctcgatcag gaaatgccgg agatggacgg cctttccacg ttagccgcta ttcgcgaaga
     4261 atggcctgcg gttcacgtga tcatgttcag ttccaggact cagcggggcg cagagactac
     4321 ggtcaaggca ttgcaactgg gagccgccga tttcctcgca aagccggcgg aaaaagacac
     4381 tgacattcct ctgtatcttc gctcgcactt gcttccgcgc attcacgcac tcggacaacg
     4441 gttctctcta agttattctt cacatatgag atccaatgtg acgggagatt tcaaagtggc
     4501 agcactcgga atttccacag gcggcccgcc ggttctaaag acgattctaa acaaactcaa
     4561 ccgcggtttg cacgcctccg ttctcattgt tcaacatatg ccgcctgtat ttacagccca
     4621 actggctcgt tctttgagtg agacttcagg aattccagtg aaggaggcag aagatgcaga
     4681 gccacttgct ccggggttcg cgtatattgc tccgggtgga cgacatctgg aagtttcgtc
     4741 ggatgggagc caattgcgca taacggatgg agatccggag ctgagctgtc gcccgtctgt
     4801 aaacgttctt tttcgatctg tcgcgcgagc atttggaccc caggcactcg ggttgattct
     4861 gacgggtatg ggagaggatg gatactcagg cattcaagaa atacgcgctc gaggtggtaa
     4921 gatcattgcg cagagtcagg actcgtgttt gatttttggc atgcccgcga gacccgtacg
     4981 cgaaggactc gtagacgccg tactttctcc ggataaaatc gcagaacgcc tcaatgaatt
     5041 atttgcggga gtgcgtacat gagtaattct ccccccaatt tgacccgcat caagacagtc
     5101 atccgcgcga agaccggtgt gatcattgat gacaagaacg aatatttgat cggaagccgt
     5161 ctgggaatcc tactcgaaga gaacaatctt tcttcctttg atgagctggc cgatgttctg
     5221 gcgggaccgc aggattctcc tatcttcaag aagttcctgg aacgaattac tactcacgaa
     5281 acacgctttt ttcgggacga aagcaccttt gcggcgttag tcgaacaaat gattccggaa
     5341 tggatggaaa gaaatagatc gttcggtggc gcttcgcgtc ctctcaatat ctggtgcgcg
     5401 gccggatcta ccggtcagga agcatactcc attgcaatgc tgattcattc taagtttcca
     5461 caaatatttc cgtctttgaa tcttgtctcc acagacatta gcgaaacatg cgtggaacgg
     5521 gcgtcggctg gatttttcac ggactatgag atgaaccgcg gagtgtcaga tgaattgcgc
     5581 gcaaaattct ttgtgcgtgt ttctgacaag ggatacaata tctcgagtga gattcgatcg
     5641 aaggtagagt tcaagcagca aaatcttctg gttacaccct atgctggcaa gtttgacatt
     5701 gttttttgta gaaatgttct gatctacttt gatctggcgg tgcgaaagca aattctagaa
     5761 ggattccagc gagtgattct cccggatgga tttctggttc tcggggcgac ggaatcgcca
     5821 acgggcttgc tggattccta cgtagtacgt cagttcggcc tcacccgcta ttatgaaatc
     5881 aattcaacaa acattacttt tgctcctgct caaatgaggt gatttatgaa aaaggtacta
     5941 gctgtagatg actctcaggc catgctaaag atggtgcaac agaccttgca gatcggcggc
     6001 tacgaagtgc tggtggcatc cgatgggaag gaagcgctgg aaaaatttcg tggtgccggt
     6061 gtggagcttc taattacgga catcaacatg cccgtgatgg acggcattac actgataaaa
     6121 gaaatcaggg ctgcggacaa ggaagtgcca atactgacct tgacgactga atctgaagag
     6181 ccaatgaagc agtctggatt tgcggcgggc gccaacgggt ggattgtgaa gccttttcgg
     6241 cctgcgcagt ttctcgatat tgtccgccag attctgagtt aatgactact cccgtcgtct
     6301 tgtttgcagg accgggcctg gatgcgctgg cagcttcgat tcgtgagaac cagtacacag
     6361 tactaacggc agaatctggc ccacaagcat tggatttgct cggagcagtt cgcgttgatt
     6421 ttttcttcgt ggctgcaggc tcgagcgccg cgccatatgt gagcgttctg cgaaagaagt
     6481 cggaagcaaa actcatcctc gtggggaatg acacgtcctc cgtgccttgc gatcgcgttt
     6541 cttctgcgca tgcggtggaa gaggctctcg ctcttctaca caggcttacc gcagacagag
     6601 caaataaaga actcatggag caggcggagg gcaggaaggt cgatgccgag caacgtcgtc
     6661 tggaatggct tacgtggaag cagcagatgg cggatcgatt gcatatgcaa tatagcgccg
     6721 ctcttgtgcg aaacattcgt cattctatga cccagggtct tggtgcaggt gctttgatca
     6781 ctcaggtgga cattcttcca cttcttcctc gcaatccttc tggcgattac attgtaccag
     6841 aatcttccgt tacttttcta ggagaagcgg cggaggccct tcgtcactgg ctttcttcca
     6901 tggaaatgtt tgtcgaagca atggaaagaa tgtatccagc tgaaacaatt cctgaagcgg
     6961 atttatctat tctcgtatcc gaagcgacgc aaagagtgga tcaccttcgc aggattaaac
     7021 gacaaaccgt tcgctctgca ttgcccacac ccgcgggtcc cattcgtatg tccaggattg
     7081 gtctttcgct cgcgatccgt gaactgttga cgaacgcatt taagtattct ccagacgcat
     7141 cgcgcgtaga cattgcgggt ttctcatcgc aaggagggta ttcaatttgt attctcaatg
     7201 atctcgtgga cttaccggcc ggaattacag gcattccacc tgaaagggaa aatgagattt
     7261 ttgaaccatt tagacgattg cacaatgtgt acgatgatcg ctttgcaatg gaagagtttc
     7321 ctatgggaac agggttgact gtggtacggc aggcaatcca gcaggcaggt gggagtgtat
     7381 cgctgtttga aattagagat cacacgcggg aagacactaa gaaaagacgc gtaatggcgg
     7441 agattttact tcccctggat atctagatat tctcgcagcg ccgcgattgt ttcttttgct
     7501 gcatcttcca gttcctcagc gttaggcgat ttttcattgg atcccggaga tttttcaatg
     7561 tcaaacagta cagatgctaa tcttgatgcc cccatcacaa ggcaaccgct tcgcgtacga
     7621 tgacacagac gagcgagttc agtcgggttc gcaccagatt gcatgagatg cgtgattgtg
     7681 ggtagccccg tcacagtatc gctttcaaat actccgaaca tcgtcgtcag agcgttgggc
     7741 gaataacgtg acagaacagt aaaatcaaga atttccattt ttatcttctg gttctccgga
     7801 gtatcttccg atcaattcga acattcgttc gaaatcgatt ggctttgcaa tgtagtcatc
     7861 catacccgct tcaaaacaac gctgcttttc tccttcgagc gcattggctg tcagagcaat
     7921 gatggcgggg gagtagttta gattttcctg gcgaatgcgc tgcgtggctg cgtatccatc
     7981 cagtcctggc atctggcagt ccatgaatat gatcttatac tgacccgtgc gggccgcttc
     8041 tagagcctcc tgtccgtttg tcgcaagatc gcacgccagt ccgcgctctc tcagcatcag
     8101 cgagatgcac atgcgattga gctcgtgatc gtccgcgact agaatgcggg ggccgttttc
     8161 aatgcgctga tggtcgggat gtttgagggg ctgtcttcca gcaggtgacg cgatgattac
     8221 ttttgattca aagcgaaacg ttgtgccctg cggatgattt gcgcgtgcag tgagagttcc
     8281 tcccatcatg actgcgagtt ctctggaaat ggataggcca agcccgctgc ccccgtagcg
     8341 cctggtggtg gacgagtccg cctgtgcgaa tgggttgaag attttgtcca gacggtcttc
     8401 ctctattcct attcctgtat ccgttacttc aaagcacagg ctgcaaattg aagtttcgtg
     8461 taaagcgcag gagcaaatcg cgcgcactta tattctgccc ttctccgtaa acttgaccgc
     8521 attgccaatc aggttgttta atatctgctt gagtcttaca ggatctccca gtatgaacgc
     8581 cggcgtttca ggtgcgagat cgtaaacgag ttcaacgcct ttttccatcg ctgagagtct
     8641 gaatagtcca gtgcattcgc tcaaaagatc cgcaacattc actggaatct tttccaggcg
     8701 cagcattcca ctttcgattc gcgaaaaatc caggatgtcg tccagaatgg ttcggaggga
     8761 tccggccgca gatgcggcca tgtcggcgta actttgctgg tcctgattga gtcccgagtt
     8821 ctgtaatagt ttaataaatc caagtattcc agtcatggga gtccggatat catgagacat
     8881 gttggccaga aatctgctct tcgctcgtgt agctgcttcc gcggcttccc ttgcctgaac
     8941 cagttccagc tgaatttccc ggcgcgcagt gatgtcgcga gcaatcccaa tgatcctcgg
     9001 acggtccgtt cgcagaatgg ttccaagcgt ttctatgtag tgaatcccat catctggcgt
     9061 tttgattctg tactgctcgt gccagattcg tctttcgctt tcaaagaatg cttgcacccg
     9121 atcagtgagc ccctggtcct caggaatgag aagtgaatcc aatgtgcgca ggttcggagt
     9181 gaaggcagac ggattttgcc cgtgtatttc aaacgctctg gcatcccaga agatgtctcc
     9241 cgagggaatg tcgatttcaa agagcgcctg acgggagatt tgcatagcca gctccaatcg
     9301 tttgcgttcc atatccaggg caatgtcgct gcgaacgcta gcgctcacat cctgcagaat
     9361 tccagaaaga ccagtgatgt cagagaagcg gcgcctcgct tcgccggcgc atcgaaccca
     9421 gatccactta cccgttgcag tctcgatctg gaattgaaca tcgaaccctg ctccccgttt
     9481 gaatgctcgg tgtaccgcac caatcaatac ttgtgcagac ccacgagaaa tcatcgattt
     9541 gatgacgggc cacgtcaggc gagcgcgagg cgaaagatca agcaactcca ggagtgtttc
     9601 tgtgcagatg atgagcccgc tctttctatc caacctccac ccgccgagct ttgcaattcg
     9661 agtggagtca atgaagagac tttcgagctc ttgttgccgt atctgcgtgg ttaccctggc
     9721 tgtaacattt tcgtggatta cggccacata cctggcttgt gcccaaacga agggaattgc
     9781 gtgcattgta taccaggtca tccctctcct ggaatggcac gggtactcat gtcgaaatgg
     9841 gcgcctccat tctgcagcag tttgcgaatg ccggacgcga tttcttctac tgcgcggtcg
     9901 tccgcggatg ctctgtcaca tacaaggaga tagttggctc cctctgtgac atcacgccca
     9961 tcgttttgtt ctgcaaatct gcgccatgcg gaattgacgg caacaatcgt tccgctggag
    10021 tccatgatcg ccacgtgagc gtcaattgcg tccagaacat gttgcgcgaa ttctgcgctg
    10081 tttgatttgt ttggacgctc ttcctcgcac ggtcgaacgg ttgccaggta gtgcgttcca
    10141 ctctccgagg tgagccgggt aacaatgata ttcgcttcga ggagctcacc gtcttttcgt
    10201 ttaactctca ctcctgtatg tggaggcatc gtttctgtgg gg
//
LOCUS       DAORFF010000050        10080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3071_length_10080_cov_3.425935, whole genome shotgun sequence.
ACCESSION   DAORFF010000050 DAORFF010000000
VERSION     DAORFF010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10080
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3071_length_10080_cov_3.425935"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PKK76_03410"
     CDS             <1..709
                     /locus_tag="PKK76_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67543.1"
                     /translation="YKSFRNLALREIAKPRRFVVVGGKTGAGKTRVLHQLCALGQPVI
                     DLEYLAGHKGSAFGGLGQKDKTQEQFENDLCISLRSLDPLCETDGGIHSGSIAWIEDE
                     SRTIGKRSVPAQIMQSKQEATLLFLDVPLKQRVENLVREYGQFPVQDLANIISRLHKR
                     LGGLDTAQALEALHAGDLERVCEIVLHYYDRAYDFELSKRSGPVIRVPVSSMDAAENA
                     SRLMQACKESVRTEAMT"
     gene            706..1749
                     /gene="selD"
                     /locus_tag="PKK76_03415"
     CDS             706..1749
                     /gene="selD"
                     /locus_tag="PKK76_03415"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNL67544.1"
                     /translation="MNTDSIRLTTYSHGAGCGCKISPRVLDEILKGNWEKPDHDRLIV
                     GNDEKDDAAVYDLGGGNGLISTTDFFMPIVDDPFDFGRIASANAISDVYAMGGRPVLA
                     LAILGWPVDKIPGEVAGRVIAGARSLCAEAGVMIGGGHSIDSPEPIFGLAVNGLVRLD
                     RLKRNSTARPGDLLFLTKPLGIGILTTAEKKGMLRTSDAGIASSVMLRLNRIGEELSE
                     IEGVHAMTDVTGFGLLGHLSGMCSASGTSAVIYSDRVRRITDLDYYLEKGCVPGGTKR
                     NFDSYGSGISAMQESVKNVLCDPQTSGGILVSVNPDSADQVRALFSKNDLAEFKEPVG
                     EIISQGAELIRVQ"
     gene            1759..2448
                     /locus_tag="PKK76_03420"
     CDS             1759..2448
                     /locus_tag="PKK76_03420"
                     /inference="COORDINATES: protein motif:HMM:NF020348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HNL67545.1"
                     /translation="MAAKKKAQKKIQNKAQKKSGKKTRAPLKKSIQASALPPMKPAVF
                     PLEKLGPLRPLVGVWEGQKGDDIAPDDSREGIENNKFRERIEFVPIIPVDNHEQKLFG
                     LRYKTTVWRLTESDPFHEEVGYWLWDPKEKQVMKSFIVPRGIVVLAGGTVEPSAQEFN
                     LSADAGSSTYGICSNLFLDREFKTVRYELNVKLIAGEFRYFEDTQMKMPGRADLFHHT
                     DENALRRSKAL"
     gene            2454..3110
                     /locus_tag="PKK76_03425"
     CDS             2454..3110
                     /locus_tag="PKK76_03425"
                     /inference="COORDINATES: protein motif:HMM:NF039832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HNL67546.1"
                     /translation="MKSRIKNRPEFKPDMGVRQFQAQYWYREELIQICRAHGLDYSGA
                     KADLEERLIGFLRGPVSPPIARQKKKTEKRKYPVSGTSREVVTRRISLDSPVSSGFAF
                     NAHAREFFAEYFKNPDFKFTMEMAEFVRDARAKGNERAKVQDLVKIYERVQREKKDGT
                     RKLPPILRWNTFVRDFNRDPKTAHLKNKMEAAAKLWGIVRDRPGPKKYDPSLLKFLKE
                     "
     gene            complement(3154..3534)
                     /locus_tag="PKK76_03430"
     CDS             complement(3154..3534)
                     /locus_tag="PKK76_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67547.1"
                     /translation="MNRKRIAIAAIAAALFLGAASYLSAESDYDFTLVNATGYDIDAV
                     YVSPSSQESWGNDVMGQDVLVNGMTVKIVFHPSAQASSWDLKVKWRDPGYPAVVWHGL
                     DLATINKLTLKYNKDTDVTSVIKE"
     gene            3705..4223
                     /locus_tag="PKK76_03435"
     CDS             3705..4223
                     /locus_tag="PKK76_03435"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNL67548.1"
                     /translation="MSGYIRQRVLGPILDLLKRGVTPHKLALCIAAGAMLGIFPVIGA
                     TMALCVIVGYLLRLNAIAIQAVNYAVYPLQIAMLVPFVRMGEWITGSDPFPVSPSAVL
                     AMLQEGIVKTLVHFSSAIIHAVLAWSLLCLPAGLLIYLAFLPILRKSAEHYARLRARH
                     AESTDDQLQAKP"
     gene            complement(4208..6001)
                     /locus_tag="PKK76_03440"
     CDS             complement(4208..6001)
                     /locus_tag="PKK76_03440"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL67549.1"
                     /translation="MILLLICALSPALAADRLSKPLTDFAYMLDPSSTIEASEMEAAR
                     DSGQFQRVKGDFIGLGFQSGSVWFDLSVRNDSARDLLYLSIPVPVLDHLELYERNGES
                     WTVQKSGDLYTREERNDFRNPTFSLSIKPGETRRYLLRIITTSAIPFHAVVSTDLAIT
                     AESESEMVWTAGYFGLLTAMILYNLFIYFSIRERSYLAYSVYVILLWMFQACNYGLAA
                     QYILPNHPVIANYAYPILASLLALSTVIFTYTFHNVHVRFLKLRKVFWATGVVCILSI
                     GATFYVQNRFNNALSIAISVLVLYTSVLSLMRGYRPALFFLAAWTGFVILTTLWSLSI
                     RGIIQNFLIFRYAFLVGSATEVVILSLALGDRINLLDSEKRAALAEQIQLKTRLLDAF
                     SRFVPRQFLRSLGKESPEEVNLGDASAAEVTILFSDIRGFTGMSERMNVTENFNFLNS
                     YLKRMEPVIESRGGFVDKYIGDAIMAIFLGNPEHAIHAAEDMMKALEEYNAHRASQNF
                     EPIKIGIGIHRGPVMLGTVGSQKRMDTTVIGDSVNACARIESLTKKIAPVLISEDALA
                     GVSDQIRNKLKRIARVQVRGKTQSLVLYGFA"
     gene            6154..6522
                     /locus_tag="PKK76_03445"
     CDS             6154..6522
                     /locus_tag="PKK76_03445"
                     /inference="COORDINATES: protein motif:HMM:NF025197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HNL67550.1"
                     /translation="MKKKFVMTVLALSAMVASPLLAKDMYGALAYSKADGAWGWWADA
                     TTQAAASKNALAKCSEHSSGCEVVLEFWNTCAAFATGSGGSYGWAHGPDENQVQSKAV
                     DFCSEHGSGCEVRVWACTSR"
     gene            6621..7259
                     /locus_tag="PKK76_03450"
     CDS             6621..7259
                     /locus_tag="PKK76_03450"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNL67551.1"
                     /translation="MGIPGLKLKLTLQQKALAFIFAVVIGTSAITAYSSYMATHMVIE
                     NCKEGDLRTAATLIQNDMQEQSNKSLARATMVARMPVVINSMRTQSREPLVDALVPMF
                     LILREKYGVREGQFHLAPATSFLRIYDVKAGHGEDLSGFREMVLMANKNKLPYSGVEI
                     GRRGLSIRGIDVITDGTDYFGSVEIGMDFGAIINNVKKNAGFEAGVFVDDRK"
     gene            7256..7717
                     /locus_tag="PKK76_03455"
     CDS             7256..7717
                     /locus_tag="PKK76_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67552.1"
                     /translation="MSEIATLIPKPDAERIIGGYRNTESTDWNMIRTVVTPDFLRSVT
                     DVTTRQQTIGGVDYGFVAVPLLDFKGTKIGSVVAVRNFSEFATQNMWALVRSISLAFI
                     QGLILCGVMLILINSLFIHPEKENAEKRRQAALNKDADPGLIPGQSGDADD"
     gene            7710..8765
                     /locus_tag="PKK76_03460"
     CDS             7710..8765
                     /locus_tag="PKK76_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67553.1"
                     /translation="MIRTLFFAAAAFFLSGPLSAESVPTGTESPLRVFVSAIVSQIGK
                     VNDQAGNFEMTVDLQLRWRDPRLKFDAREHGSDRKAFLGDEAEAETKEIWTPQLVIRN
                     MNGSPIRLEQAITIQSDGTVVHLQRIKASFDTKFRLQAFPFDKQELPVRLISNKFNSN
                     QIVLTQDQTDKDTSGIKQGVGVSGFHLQKIAFSPGRERGLNGEYFPTMDARVQIARDP
                     TSHIFTLFAPFLALLVIPTVLTLFAKVDVAPRLTAWSASILALIALNFTFSIRYPAVG
                     ADGLINSVVSTGFGYQLLMVFISLIVLYPPVAEKITAKLGNKYVIAEVEKFMKWGIPV
                     GLFALLISSVLLTMYTT"
     gene            complement(8776..>10080)
                     /locus_tag="PKK76_03465"
     CDS             complement(8776..>10080)
                     /locus_tag="PKK76_03465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNL67554.1"
                     /translation="RILIFLNNQQVAAIGAARGTGNIWTGFHWIDLPDVAGESVLELE
                     ITGAYDLGIREVPYLAPSGDIALFRWVTSVLFNELYWLVLGLILALGALQIYYAQIDP
                     RLRKTYWLNTAGILLGGFYLFDFAIRSDSGTMTSYLWARKLMLSAGYAAPLLMLMAME
                     YYTRKKLYFSKFTLLTTAVAAGLAISAGSLSDLKKWSVLTAGLAQLNWLFLLIIAFRT
                     RHNVIVFAVTFFYCTLVGSVFSMISPSSHIFMIQIGYVFAITSVVYNMIEEFGRVHED
                     YRTAYRKSRTDPLTGAYNRFILDEVTVEPGDAVVLIDMNNFKVINDAFGHHAGDRVLV
                     EWVKSAQLHTDQSDMIIRTGGDEFIILVRGARETDMNRILEDFQQRVSDLPASFSYGI
                     EKIQTTLHDAIQNADQRMYAHKKNSRSTWIRRLWQRRQKKTA"
BASE COUNT         2529 a         2373 c         2714 g         2464 t
ORIGIN      
        1 ctacaaaagc tttcgcaatc tggccctcag agaaatcgca aagccaagac gctttgtagt
       61 cgtaggcggg aaaacaggcg cgggcaaaac aagagttttg catcagttgt gtgcacttgg
      121 acagcccgtc atcgatctgg aatatctggc gggccacaag ggatccgctt tcggcgggct
      181 tggacaaaag gacaagacgc aagaacagtt cgaaaatgat ctatgcatct ctttgcgatc
      241 gctcgatccg ttgtgcgaaa ctgacggggg cattcacagc ggatcgatcg cctggatcga
      301 agacgaaagc aggacgattg gaaagcgaag cgtgcccgcg caaatcatgc aatccaagca
      361 ggaagcgact cttttgtttc tggatgttcc actcaagcaa agagtggaga acctggtgcg
      421 agagtacggc caatttccag tgcaagatct tgcgaacatt atctccaggc tgcataagcg
      481 actcggtggc ctcgacacag cgcaggcgct tgaggcgttg cacgcaggag atctagaacg
      541 agtgtgcgaa atcgttttgc attactatga tcgagcctat gattttgagc tttcaaaaag
      601 aagtggccct gtgattcgag tcccggtgtc ttcgatggac gcggcagaaa acgcatccag
      661 gttgatgcag gcctgcaagg aatctgttcg cacagaagca atgacatgaa cacagattcg
      721 attcgcctca ctacatacag ccacggggca ggctgcggtt gcaaaatttc gcctcgcgtt
      781 ctggatgaaa ttctcaaagg caactgggag aaaccggacc atgatcgctt gattgtaggc
      841 aatgatgaga aagacgatgc tgcagtctat gacctgggcg gtggaaatgg actcatcagt
      901 actacagatt tcttcatgcc tatcgtggac gatccttttg attttggaag aattgcatcc
      961 gccaatgcga tcagcgacgt ctatgcgatg ggaggtcgtc cggttctagc actcgcgatt
     1021 ctgggctggc ccgtagataa gattcctgga gaagtggcgg gaagagtgat tgcgggcgca
     1081 agatcccttt gcgcggaagc tggagtgatg atagggggcg ggcacagcat cgatagcccg
     1141 gagccaatct tcggtcttgc agtcaatggg ctcgttcgcc tggatcgatt gaaaagaaat
     1201 agcaccgctc gtccggggga tctgttgttc ttaaccaagc ctctggggat tggaatactg
     1261 accactgctg aaaaaaaagg gatgcttcgc acatcggatg cagggattgc ttcgagtgtg
     1321 atgcttcgat tgaatcgcat tggagaagag ctttcggaga tagaaggtgt ccatgcgatg
     1381 acggacgtga caggatttgg tctactgggt catctgagtg gcatgtgttc tgcgtcagga
     1441 acatccgcgg tgatctactc ggatcgagtc agaaggatca cggatctgga ctactacctg
     1501 gagaaaggtt gtgtgcccgg tggaaccaaa cgaaattttg acagttatgg aagcggaatt
     1561 tctgccatgc aagaatccgt gaaaaacgtg ctctgcgatc cgcagacgtc aggcggcatt
     1621 ctggtttccg tgaacccgga ctccgcagac caggtgcgcg cattgttttc gaaaaatgat
     1681 ctggcggaat tcaaagagcc agtgggtgaa atcataagtc agggcgcgga attgattcgc
     1741 gtccagtagg aagatggaat ggcggcaaag aagaaggcgc aaaaaaagat ccagaacaaa
     1801 gcccagaaaa agagcgggaa aaagacgcgc gcccctttga aaaagtccat tcaggcaagc
     1861 gctctgccgc ccatgaagcc tgcggtcttt ccactggaaa agcttggtcc gctgagacca
     1921 ctggtgggag tgtgggaagg ccagaaggga gatgacattg ctccggacga ttcgcgcgag
     1981 ggaattgaaa acaacaaatt tagagagcga atcgagtttg tacccatcat cccggtagac
     2041 aatcacgaac agaagctatt cggcctgcgc tacaaaacaa ccgtctggag gcttactgaa
     2101 tcagatccat ttcacgaaga agtaggttac tggctttggg atcctaaaga aaagcaagtg
     2161 atgaaaagct tcattgtgcc ccgtggaatc gtagttctgg cgggtggaac ggtggagcct
     2221 tctgctcagg aattcaattt gtccgcggac gctggatctt ctacctacgg gatctgttcc
     2281 aatttgtttt tagaccgcga attcaagaca gttcgctatg aactcaatgt gaaactcatt
     2341 gccggagaat tcaggtattt cgaagacacg cagatgaaga tgccggggag agcggatcta
     2401 tttcatcata cggatgaaaa cgcactgagg cgcagcaaag cactctagac cggatgaagt
     2461 ccagaatcaa gaatcgccct gagtttaaac cagatatggg agtgcgccag tttcaggcgc
     2521 aatactggta tcgggaagaa ttgattcaaa tttgtcgcgc gcacggactg gactacagtg
     2581 gagccaaggc agatctagaa gagaggttga ttggttttct gcgcggcccg gtctcgccgc
     2641 ccatcgccag acaaaagaaa aagacggaaa agcgaaagta tccggtctcg ggcacatcga
     2701 gagaggttgt caccaggcgt atctctcttg attcaccagt atcctccggc tttgctttca
     2761 acgcgcatgc cagagagttc tttgccgaat acttcaagaa tccggatttc aagtttacta
     2821 tggagatggc agagtttgtg cgcgatgcgc gtgcgaaggg aaacgagcgt gcgaaagtcc
     2881 aggatctggt gaagatttac gagagggtgc agcgggagaa gaaagatggt acgcgaaagc
     2941 ttccgccgat tttgcgctgg aatacctttg tacgcgattt taaccgcgat ccgaaaacgg
     3001 cacacttgaa aaacaaaatg gaagctgcgg ctaaactgtg gggaattgtg agagatagac
     3061 cgggaccaaa gaagtatgat ccgtctcttt tgaagtttct gaaagagtag aggcattcac
     3121 cccggggcaa tgccccggga tgcaatgcaa agattactct ttgattacgg aagtaacgtc
     3181 tgtgtctttg ttgtacttca gagtcagctt gttgatggtt gccaggtcca ggccatgcca
     3241 tactacagca ggatatcctg gatcacgcca ttttactttc agatcccagg aagatgcctg
     3301 agcagatgga tggaatacga tttttacagt catgccgttt acaagaacgt cctgacccat
     3361 tacgtcattt ccccagctct cctggctgga tggcgataca tacactgcat cgatgtcgta
     3421 gccagttgcg tttacgagcg taaagtcgta atctgattcg gcggaaaggt agcttgccgc
     3481 gccgagaaac agtgcagctg caattgctgc gattgcgatt ctctttctat tcattttttc
     3541 ctctcaagat agtgggccga ggcccggctt attggaattg cctgcgtttc gccggcaaca
     3601 attataggac gaacgaatcg tgcgaaacgt gaattttttt atttttgaga tggagcgtcg
     3661 tatccgtaga aacacggatc tgccggtgga agcgcggtga tctcttgagt ggctacattc
     3721 gacaaagggt gctcgggccc attctggatc tgctcaagag aggggtgacg cctcataaac
     3781 ttgcattgtg cattgcagca ggcgcgatgc ttggaatctt ccctgtgatt ggtgcgacca
     3841 tggcgctttg cgtcatcgta gggtatctac tccggttgaa cgcgatcgcc attcaggccg
     3901 tgaattacgc agtataccct cttcagatcg ccatgctcgt tccttttgtg cgaatgggag
     3961 agtggatcac aggctcagac ccgtttccag tttcgccttc tgcggtgctt gccatgttgc
     4021 aggaaggaat tgtgaaaact ctggtccatt tctccagtgc gatcattcat gcagtacttg
     4081 catggagcct cctttgtctg cctgcgggat tgctcatcta tctcgcgttt ttgccaatcc
     4141 ttcgcaagag cgcagaacac tacgcgaggt tgcgcgctcg ccacgcggaa agcactgacg
     4201 accagcttca ggcgaagccg taaaggacta gagactgagt tttcccacgc acctgcacgc
     4261 gcgcaattcg cttgagttta ttcctgatct ggtcagatac acctgccagt gcgtcctctg
     4321 aaatcaaaac aggtgcgatc ttcttggtca gactctctat tcgcgcgcat gcgttgactg
     4381 aatcgccgat cacagttgta tccattctct tttgcgaacc gactgttcca agcatcacag
     4441 gaccgcgatg aattccgatt ccaatcttga ttggctcaaa gttctggctg gctctgtgcg
     4501 cgttgtattc ttcgagtgct ttcatcatgt cttccgcggc atggatggca tgctccggat
     4561 ttccaagaaa gatcgccatg atcgcatctc caatatactt atccacgaat ccgccccggc
     4621 tttctattac gggttccatt cttttgagat agctattgag gaaattgaag ttctccgtca
     4681 cattcattct ctcagacatt ccagtgaaac cgcgaatatc ggagaacaga atggtgactt
     4741 ctgctgcact cgcatctccg aggttcactt cttcggggct ttccttgccc agagaccgta
     4801 agaactgccg cggaacaaaa cgagagaacg catccagaag ccttgttttt aactgaattt
     4861 gttctgccag tgccgctctc ttttcagagt cgagcagatt gatgcgatcg cccagagcca
     4921 gagacaggat cacaacttct gttgcagaac ccacaaggaa tgcgtagcga aagatcagga
     4981 aattctgaat gataccgcga atggagagac tccacaatgt agtgagtatt acaaatccag
     5041 tccaggcggc caggaagaac agtgctggtc tgtacccgcg cattagactg agaacagatg
     5101 tatagagaac cagaacgctg attgcaatgg acagagcatt gttgaatcga ttctgcacat
     5161 agaaagtggc gcctatgctg aggatgcaga ctacacccgt ggcccagaat acttttcgca
     5221 gcttcaggaa gcgaacgtgc acattgtgaa acgtatatgt gaagatgaca gtggagagtg
     5281 cgagtaaaga agcgaggatg ggataggcgt agttcgcgat gactggatgg ttgggcaata
     5341 tatactgtgc tgccagtcca tagttgcatg cctgaaacat ccacagtaag atcacataga
     5401 cagagtaagc caggtaggat ctctctcgga tggagaagta gatgaacaaa ttgtacagta
     5461 tcatcgcggt gagcagacca aagtagcctg cagtccagac catttcggat tcagactccg
     5521 ctgtgatggc aagatccgtg gaaaccactg catgaaacgg gatcgcgctt gttgtgatga
     5581 tgcgaaggag gtagcgtctg gtttctcctg gttttataga tagagagaaa gtggggttgc
     5641 gaaagtcgtt tcgttcttct cttgtgtata gatcgcctga tttttgtacg gtccacgatt
     5701 ctccgtttct ctcataaagt tccagatgat ccagtactgg cactggaatg ctgaggtaga
     5761 gaaggtctcg tgcggaatcg ttacgaacgc tcagatcgaa ccacacagat ccggactgaa
     5821 atccaaggcc tatgaaatcg ccctttactc tttgaaactg gccgctgtcc ctggcggctt
     5881 ccatttcgct tgcctcgatg gtggacgatg ggtcgagcat gtaggcaaaa tcagtgagtg
     5941 gcttgctcaa gcgatctgct gcgagggcag gggacagggc acagatcaga agcaggatca
     6001 ctggcagccg catatgtctg agtcgatggg ggctcctgga tcttacaatg tgaaaatcca
     6061 ccgccgggcg gtcgccagag cgaaaaaaca gttgaacaaa aacgcgaaac gggctgtctc
     6121 tcgttctggg ttcattccca cggaggagtt cgtttgaaaa agaaattcgt tatgacagtt
     6181 ctggctctca gtgctatggt tgcttcccca cttcttgcta aagacatgta cggtgctctg
     6241 gcttattcca aagcagacgg tgcatggggc tggtgggcag acgcaaccac tcaggctgca
     6301 gcaagcaaga acgcgcttgc gaaatgttca gagcacagtt ctggctgcga agtggttctt
     6361 gagttctgga acacttgcgc cgcatttgca actggcagtg gcggatcata tggctgggca
     6421 catggaccag atgagaatca ggttcagtcc aaggctgttg atttctgcag cgagcatggt
     6481 tctggctgcg aagtaagagt ctgggcttgc accagtcgct gacttaaaga aagggaggac
     6541 gtccgtcctc cccgattttt catcgaactt ccgggatttt agttgcgcga cccactccgt
     6601 ggggccattc gaaccacttt atgggaatcc caggcctcaa actcaagctc actctacaac
     6661 aaaaagcact cgcattcatt ttcgcagtcg taattggaac cagcgccatc acggcgtatt
     6721 catcctacat ggccactcac atggtcatcg agaattgtaa ggaaggggac cttcgcacag
     6781 ccgccactct cattcaaaat gacatgcagg agcaatcgaa caagtctctt gcgcgcgcca
     6841 caatggtggc acgtatgccc gtcgttatca attccatgcg aacgcagagt agagagccac
     6901 tggtggacgc gctggttcct atgtttctca tcctgagaga aaaatacgga gtgcgcgaag
     6961 gccagtttca tctggctccc gcgacttctt ttctccggat ctatgacgta aaggccggtc
     7021 acggggaaga cctcagcggc tttcgcgaaa tggttctcat ggcaaacaaa aacaaactgc
     7081 cgtattcagg agtcgagatt ggaagacgcg ggctcagcat tcgcggcatt gacgtgatca
     7141 cggacggaac ggactacttt ggcagcgtgg aaatcggaat ggatttcgga gccatcatca
     7201 acaacgtaaa gaagaacgcg ggttttgaag cgggcgtgtt tgtggatgat agaaaatgag
     7261 cgaaattgcc accctgattc cgaagccgga tgcggagaga atcattggcg gatatcgaaa
     7321 tacggaatcc actgactgga atatgattcg cactgtggtc actccagact ttctgcggtc
     7381 tgtgacagat gtgaccacga gacagcagac gataggcggc gttgattatg gctttgtggc
     7441 tgttcctctc cttgatttta agggaacaaa gatcggttct gttgtcgcgg ttcgcaattt
     7501 ttcagagttc gcaactcaaa acatgtgggc cctcgtgcgt tccatctctc ttgctttcat
     7561 tcaagggctg attttgtgcg gagtcatgct gatcctgatc aactctctct tcatccatcc
     7621 tgagaaagag aatgcggaga aaagacgcca ggccgctcta aacaaagatg cagatccagg
     7681 gctcattcct ggacagagcg gagacgcaga tgattaggac tctctttttc gccgcggctg
     7741 cattcttcct gtctggcccg ctctctgcgg aaagcgtccc tacaggaaca gagtcacctc
     7801 tccgcgtttt tgtttctgcc attgtaagtc agattggaaa ggtcaatgat caggccggaa
     7861 actttgaaat gaccgtcgat ctgcagctgc gatggcgaga tcctcgtctc aaatttgatg
     7921 ccagagaaca tggctcagat agaaaggctt ttctgggaga tgaggcagaa gcggaaacaa
     7981 aagagatctg gacccctcaa ctggtgattc gcaatatgaa tggatctccc attcgtcttg
     8041 agcaggcgat tacgattcaa tcggatggca ctgtagtgca tttgcaaagg atcaaagcta
     8101 gttttgatac taagtttcgc ctgcaggcct ttccgtttga taagcaggag cttccggttc
     8161 ggctcatatc caacaagttc aatagcaatc aaatcgttct gactcaggat caaacggaca
     8221 aagacacatc tggaatcaag cagggtgtgg gagtgtcggg gtttcacttg cagaagatag
     8281 ccttttctcc cggccgcgag agaggactca acggtgagta tttcccaacg atggatgcgc
     8341 gcgttcagat cgcgcgtgat cctacttctc acatattcac tctgtttgct ccttttctgg
     8401 cacttctcgt aattccaacc gtgctgactc tgtttgcgaa agtggatgtt gcgccaaggt
     8461 tgactgcatg gtccgcgtct atcctggcgt tgattgcgtt gaacttcaca ttctccattc
     8521 gttacccggc cgtgggcgcg gatggtctga tcaattcagt tgtttccact ggatttggtt
     8581 atcagcttct gatggttttc attagcttga ttgtactcta tccaccggtg gcagaaaaga
     8641 tcacagcaaa gcttgggaac aagtacgtga ttgcggaagt ggaaaagttc atgaagtggg
     8701 gcattccggt gggtttgttt gcgctattga tcagcagcgt gcttttgacc atgtatacga
     8761 cttgatgttt tgaactcagg cagtcttttt ctgcctgcgt tgccagagtc ttcgaatcca
     8821 ggtggacctg gaattctttt tatgcgcata cattctctgg tccgcgtttt gaatggcatc
     8881 gtgcagagtg gtttgtatct tttcgattcc gtaggagaaa gacgcaggca aatctgaaac
     8941 tcgctgctgg aaatcttcca gaatgcgatt catgtctgtc tctcttgctc ctcgcactag
     9001 aatgatgaat tcatctccgc cggtgcgaat gatcatatcg ctttgatctg tgtgcagttg
     9061 cgcgctcttc acccactcta caagcacgcg atctcctgcg tgatgtccga atgcatcgtt
     9121 gatcactttg aaattgttca tgtcaatgag aacgactgcg tctcctggtt ctaccgtcac
     9181 ctcgtcgagt atgaatcgat tgtaggcgcc tgtgagagga tctgttcttg attttctata
     9241 tgcagtgcga taatcctcat gcactcgccc gaattcttct atcatgttgt acacaacaga
     9301 tgtaatcgca aagacgtatc ctatctggat cataaaaatg tgagaagatg gtgagatcat
     9361 agagaagaca cttccgacca gagtgcaata gaagaaagtg actgcgaaaa cgattacgtt
     9421 gtgacgagtg cgaaacgcaa tgatcaagag aaaaagccag ttgagctggg caagccccgc
     9481 cgtaagcaca ctccatttct tcagatcaga aagagatccg gccgaaatcg caagccctgc
     9541 agccaccgca gttgtcaaaa gagtgaactt tgagaagtaa agcttcttgc gagtgtagta
     9601 ctccattgcc atgagcatca gaagaggcgc cgcatatccc gcagaaagca tgagttttct
     9661 ggcccagagg taggaggtca tggtgccgct gtcgcttcga atcgcgaagt cgaacagata
     9721 gaagccgcca agcaaaattc ctgcggtgtt gagccagtag gtttttcgca gccgcgggtc
     9781 aatctgcgcg tagtagattt gaagcgcccc cagggcgagg ataagcccca ggaccagcca
     9841 gtagagttcg ttgaacagga cggaagtgac ccatcggaac agagcaatgt cgcctgacgg
     9901 ggccaggtat ggaacttctc tgattcccag gtcgtatgcc ccggtaatct ccagctccag
     9961 aacggattct ccagccacgt ccggcaagtc gatccagtga aatccagtcc agatgtttcc
    10021 ggttccgcgc gcggcaccaa tcgcagctac ctgttgattg tttaaaaata ttagtattcg
//
LOCUS       DAORFF010000051        10044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3090_length_10044_cov_3.488738, whole genome shotgun sequence.
ACCESSION   DAORFF010000051 DAORFF010000000
VERSION     DAORFF010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10044
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3090_length_10044_cov_3.488738"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PKK76_03470"
     CDS             <1..205
                     /locus_tag="PKK76_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67555.1"
                     /translation="PPHEVLTWPRVTASDAEVRSLLFGQTPRLQMPSGSQFAWIVGAD
                     GLPVAWAEVLDYGLYRVRRIMKG"
     gene            complement(240..863)
                     /locus_tag="PKK76_03475"
     CDS             complement(240..863)
                     /locus_tag="PKK76_03475"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNL67556.1"
                     /translation="MRRKARATLCIAFVLASACSRYPAPTYMAMALLAPPAASKRLFT
                     TTTVTLGSIAGVAGADSICASDTARPTAAVYKALIVDSVGNRRACATSNCSGGISENI
                     SWVLRPNQAYVRADGSTPVMQTNSAGIFDFTFASLTNSIDGTTNRFWTGLATDWTVAA
                     ATHCSDWTSSSGVVNGERGFPNNVTAAAISSGAVACNAARAILCVEQ"
     gene            1028..1330
                     /locus_tag="PKK76_03480"
     CDS             1028..1330
                     /locus_tag="PKK76_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67557.1"
                     /translation="MRIHLRAFSISLTTIFSLLILILTIWTRLSSSFGSDFMSAFQSI
                     HPNPYGANHSDLTLGEQALGSMFDLFYAATDSLVFALGFGSLYNFLVNKLSKSDSE"
     gene            1330..2250
                     /locus_tag="PKK76_03485"
     CDS             1330..2250
                     /locus_tag="PKK76_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67558.1"
                     /translation="MLETGLHKVPVAAAESFAKTVRLLALSGRRAFTTYLVHPLDEAG
                     WKRNAAVDSTKKMLVRIKAASMDPSAVHTVAPHCKRLISQALNETLSAVGDCTIFFLD
                     MMLAHTPVSTAPEAIDFALILDRPLEKFQKESETKNTNLFTDALSSMSKEDFKKAFAP
                     VDLGKTEQQVIVKQEAAILFNKIQIAHRRDDLPACRKLIARYMVQNAESQDQNLSIVR
                     DLIKAFDTRVPGFRAELNQFMAIHLHYDVVAGISQGDIKKAIRGIRKYAHIFQGNPEV
                     PYHMEIDRMEGKLYEIISQKGLWEELKKGE"
     gene            2252..2590
                     /locus_tag="PKK76_03490"
     CDS             2252..2590
                     /locus_tag="PKK76_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL67559.1"
                     /translation="MDLQITSQAAGSVTVLKMVGKLDAQSAPAANDQLKALIGSGKTK
                     VVLDLDGLTYISSAGLGILNANQAEARKAGGMIRLACVKPQVKDVFDLLKFTLLFPMF
                     GTVQDALKDF"
     gene            2592..3002
                     /locus_tag="PKK76_03495"
     CDS             2592..3002
                     /locus_tag="PKK76_03495"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL67560.1"
                     /translation="MAAAVHEFKSALEELDRFRAVIDDFVSTDLGEIEKNRVILALDE
                     AFVNVITHGYAQDQNKPIELKMDRKGDGFEFTLLDRAPLFDPLKAVSPDLAGHAESGK
                     NHGLGIHLYTTIMKTTHSARPGGGNILTMFKAAT"
     gene            3002..5365
                     /locus_tag="PKK76_03500"
     CDS             3002..5365
                     /locus_tag="PKK76_03500"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL67561.1"
                     /translation="MGLRARITLFTGVVLLIVVSVLLAAVSREQERLFEVRVQRETSP
                     YFLPVRKLVTESAHFQKNMVRLSEARFVNENLDSKLAGKGGLKARFVFNEDYFKTAES
                     RIRSLLPDILEKKGDEWISLSRSAALKKGTPEFADYARYLENRLLLQQNFGEVYLKSL
                     TGLDRRVFRIQALDSAGQIRAAGAGADTQAISDTYNDAWINKLTLNADPPVSALYTIQ
                     SSEVQADGHKFLAFTSAIARNEEIGFRGRALLSAAGTRKLTSLIALDRKYCVALKDAG
                     QKVLAREDALVSQETPVLPAYDAEYKKLSDAYAALLRERQDASVRALKRISADDEESR
                     GALEDAVKNLRGAVLDANLFIRFDYSSYARGYSGREDWRTQFENRYNYMRKWILDGGV
                     EVYYPYAGAGEHLLFNRRNALERMITIDTTPIDDLASQALREESAGFVRIFVDPAKFQ
                     EDRAQERDRILDTALAFGLRIFFVAMLLSAFLVRRIQEIISGARQVGVGNLAVTFETR
                     GSDEVTDLARSLNQMVDGLREREAMRGEMSAAEEIQRRLLPDRMPDNMAEALSFGTFY
                     KAMMGVGGDYFDLMEAGPASMVFCIADVSSHGSGPAIVMTMLRSYLRAAVKRTRDLRK
                     IVLDLNARMFEETPSHMFITMFLGLYDRDKHTIEAVNCGHNKSFIYKYSSGAIEEFES
                     TSLPLGAVDSDLFATAVTTQTIKLSQGDLFFQYTDGLNEGMNAAGEEFGLLRVKDILR
                     ENGRKKPVILLEMIAKAAEEFTGKKIFVPGPSEQKDDIAMIAFRRMK"
     gene            5432..6682
                     /locus_tag="PKK76_03505"
     CDS             5432..6682
                     /locus_tag="PKK76_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNL67562.1"
                     /translation="MRRILLALSIGILWFGSPSCLSRCIGGAHDFKEGSKYSSLSPRA
                     QELIRLAFSDLDAKKLMDYHLHIVSTGTTCKECFINPNFYSSLHPIKRMRFKVYVSAA
                     GVTDLTRTDEQYVERLLALIDNMEEHGRFLILAFDKHYTEQGEVDSESTDFYVSNDYS
                     MQVASRSRFLVPAVSVHPARKDALVELERVAKKGARVVKWLPNAMGIHPDDPSYKPFY
                     AMMKKYDMILLSHAGEERAVDAQEYQELGNPLYLRAPLDAGVKVIVAHCASLGQAQDL
                     DAPGHPLVSNFDLFVRLFSEKKYTGRLFADISAMTQFNRLGSHPEPEPLKYILAHPEM
                     HDRLVNGSDYPLPAINIVIRTKALMQNGFISTEERESLNEIYKFNPLLFDFVVKRTLH
                     HPEHADWKLSPGIFLEKKELRPFQ"
     gene            6787..7062
                     /locus_tag="PKK76_03510"
     CDS             6787..7062
                     /locus_tag="PKK76_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67563.1"
                     /translation="MLNSLQIYERLRHARVDTRAAREIASVFGDLMDHQIAGKRDLAE
                     TETRVKAEIKVEIHRAKSETLKWMILWVSGLLAAQTGMIMAAIRFVR"
     gene            7136..7414
                     /gene="rpsO"
                     /locus_tag="PKK76_03515"
     CDS             7136..7414
                     /gene="rpsO"
                     /locus_tag="PKK76_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNL67564.1"
                     /translation="MTELQEKKDRTKTLIREFGKNERDTGSTEVQIALLSERIKALTD
                     HFKINTRDHASRRGLLMLVGQRRRLLEYLKGRDQDKYKDLINKLGLRK"
     gene            7465..9564
                     /gene="pnp"
                     /locus_tag="PKK76_03520"
                     /pseudo
     CDS             7465..9564
                     /gene="pnp"
                     /locus_tag="PKK76_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
     gene            9769..>10044
                     /locus_tag="PKK76_03525"
     CDS             9769..>10044
                     /locus_tag="PKK76_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNL67565.1"
                     /translation="MARIRIALKQGARIPARSTDGAAGYDVCALLPPDTEIIIAPGQR
                     MLVPTGIFLDIPEGYFVTLRPRSGLALKNGITLLNSPATIDWDYRGEL"
BASE COUNT         2567 a         2452 c         2635 g         2390 t
ORIGIN      
        1 ccctccgcat gaagttctta cctggcctcg cgtgacggcg agcgatgcag aagtgcgcag
       61 tttgttgttt ggacaaactc ccaggttaca gatgccatcc ggaagtcagt tcgcctggat
      121 cgtgggtgca gatggcctcc cggttgcatg ggcagaagtc ctggactacg ggctctatcg
      181 cgtgcggcga atcatgaaag gctgaggccg ggttcctttt ccgggcgcgg agtgcgaaat
      241 cactgctcca cacaaagaat ggcgcgcgct gcattgcatg cgactgctcc agaggaaatg
      301 gcagcggccg tgacattgtt tgggaatccc ctttccccat tgaccactcc ggaactcgat
      361 gtccagtctg agcagtgtgt ggccgcagct acagtccaat cagtcgccag tccggtccag
      421 aatcgatttg tggttccgtc aatggaattg gtcaggcttg cgaacgtgaa atcaaatatg
      481 cccgcagaat tggtctgcat gacaggagtg ctgccgtccg ctcgaacgta ggcctgattt
      541 ggtctgagaa cccaggaaat attttctgag attccgcctg agcagttcga agtcgcgcag
      601 gcccggcgat tgcccacgct gtccacgatc aaggctttgt aaaccgcggc cgtaggacgt
      661 gctgtatcag atgcacaaat cgaatctgca cccgccactc cggcgatgga accgagagtc
      721 acagtagtag tagtgaatag tcgttttgat gccgcgggtg gtgcgagtaa ggccatcgcc
      781 atgtacgtgg gcgcggggta acggctgcac gccgacgcaa gaacaaaggc aatgcacaga
      841 gtggcgcgtg ctttccttcg catgcccatc gcatggcttg ttggtccagg agatctatcc
      901 ttttttcttt tcccttctgc gcgcagggcg tcgcgcttga gatagacact ccctggagcg
      961 cctgtcacat ttttaaagat ccaaatgggc ttgactcaac tgcgggcgcg tcttgatgtc
     1021 gaagcgtatg agaatccatc tgcgtgcttt ttccatcagc ctcactacta tcttttcgct
     1081 tttaatcttg attctcacca tctggactcg cctctcgagc agctttggct ccgacttcat
     1141 gagcgcattt cagtccattc atcccaatcc atacggagcc aatcattctg atttgaccct
     1201 gggcgagcag gctcttggtt ctatgtttga cttgttctac gctgccaccg attcccttgt
     1261 ctttgcactc ggattcggaa gcctgtacaa cttccttgtg aacaaattat cgaaatcgga
     1321 ttctgagtga tgctcgaaac gggtttgcac aaagtcccgg ttgcggcggc tgagtcgttc
     1381 gccaaaacgg tgcggctcct tgctctttcc gggaggcgcg cattcacaac gtatctggtg
     1441 cacccactcg atgaagcagg ctggaagcga aacgctgcgg ttgactctac aaagaagatg
     1501 ctcgttcgca tcaaagctgc gtccatggat ccttctgctg ttcacactgt ggcacctcat
     1561 tgcaagcgtc tcatttcgca ggcgctcaac gagactcttt ctgcagtagg agactgcacg
     1621 atcttctttc tggatatgat gcttgctcat acacctgttt cgaccgcacc ggaggcaatt
     1681 gacttcgcac tgattctgga caggcctctt gagaaatttc agaaagagtc tgaaacaaaa
     1741 aacacaaacc tgttcacgga tgctctctca tccatgagca aagaggattt caaaaaagca
     1801 ttcgcgcccg tggatctggg taaaacagaa cagcaggtca tagtaaaaca ggaagcggcc
     1861 attctcttca ataaaattca gattgctcat cgcagagatg accttcctgc gtgcagaaag
     1921 ctcatcgcca ggtacatggt tcaaaacgcg gaaagtcagg atcagaatct ttccattgtc
     1981 cgtgatctaa tcaaggcctt tgatacgcgc gttccaggat ttcgcgcaga actcaaccag
     2041 ttcatggcca ttcatctaca ttacgatgta gttgccggaa tcagtcaggg agacattaaa
     2101 aaagcaattc gcggaattcg caaatacgcc cacatcttcc agggaaatcc ggaagttcct
     2161 tatcacatgg aaatagacag gatggaagga aagttatacg aaatcatttc gcagaaaggt
     2221 ctgtgggaag aattgaagaa aggagaataa cgtggactta caaattacat cacaggcggc
     2281 aggctccgta actgtactga agatggtagg aaaactggat gcacaatcgg caccggcagc
     2341 aaacgatcaa ctaaaagcac tgattggttc tgggaaaaca aaagtggttc ttgacctgga
     2401 cggtctcacg tacatttctt ctgcgggact gggcatcctc aatgcgaatc aagcggaagc
     2461 acgcaaagca ggagggatga tccggctggc ctgtgtgaag cctcaagtga aagatgtgtt
     2521 tgatcttcta aaattcactc tcctgtttcc gatgtttggc actgtacaag acgctctgaa
     2581 ggatttttga aatggcagcc gcggtccacg agttcaagag cgcgctggaa gaactggacc
     2641 gatttcgcgc agtcattgac gattttgttt ccacagacct gggagaaatc gaaaagaatc
     2701 gcgtcatact cgctctggac gaagcgtttg tgaatgtgat cactcacggt tatgcgcaag
     2761 accagaataa gccaatcgaa ctcaaaatgg acagaaaggg tgatggcttc gaatttactc
     2821 tgctcgaccg ggcacctctc tttgatcctc tgaaagctgt gagtccggac ctcgcgggac
     2881 atgcagagtc aggaaagaat cacggccttg gcattcatct ctacactacg attatgaaaa
     2941 caacgcactc tgctcgcccg ggcggcggga acattctcac catgttcaag gcggccacct
     3001 gatgggattg cgtgcgcgca taacgttgtt cactggcgtc gttcttttga tcgtcgtttc
     3061 tgtgctgctg gcggccgttt cgcgagaaca ggaacgtttg ttcgaagtga gagtccagag
     3121 agaaacatct ccgtactttc ttcctgtgcg aaagctcgtt acagaatcag cacattttca
     3181 aaagaacatg gttcgacttt cagaagctcg attcgtgaat gagaatctgg attctaagct
     3241 tgcgggcaaa ggcgggctca aagcacgttt tgttttcaat gaggactatt tcaaaacggc
     3301 agaatctaga atcaggagtc ttttgccgga tattctcgag aagaagggag acgagtggat
     3361 cagtctttct agatcggctg ctttgaaaaa aggaactcct gagtttgcgg actatgcgcg
     3421 gtatttggag aatcggctcc tcttgcagca aaactttggt gaagtgtatc tgaagtcttt
     3481 gacaggactg gacagacgcg tgtttcgcat tcaggcactg gattcggcag gccagattcg
     3541 cgcggctgga gcaggcgcag acacacaggc aatcagtgac acctacaacg atgcatggat
     3601 caacaaactc acactcaacg cggacccacc tgtttccgct ctgtacacaa tccagtcgag
     3661 tgaggttcag gcagacggac acaagtttct tgcatttacg agtgccatcg caaggaacga
     3721 agagatcggc tttcgcggta gagctctttt gtccgccgca ggcactcgca agctgacttc
     3781 actcattgca ctggaccgta aatactgcgt tgcgttgaag gacgcaggac agaaggtcct
     3841 cgcgcgcgaa gacgcgctag tgtctcagga gactcctgtt cttcctgctt atgacgccga
     3901 atataagaaa ctctccgacg cgtatgcggc actcctgcga gaaagacagg atgcatctgt
     3961 gcgcgcactc aagcgaatct cggcagatga tgaggaatcg cggggtgcac tcgaggatgc
     4021 ggtcaaaaat ctcagaggcg ccgtgctcga tgcaaatttg ttcatccgct ttgactactc
     4081 atcgtatgcg cgcggttatt ctggacgcga agactggaga actcagtttg aaaatcgtta
     4141 caattacatg cgaaagtgga ttttagacgg cggcgtagag gtatactatc cctatgcagg
     4201 agcgggagag catcttctct ttaacagaag aaatgcactg gagcggatga ttacgatcga
     4261 cacaacgccg atagacgatc ttgcttctca ggcactcaga gaagagtccg cgggatttgt
     4321 gcgcatcttt gtggatccgg ctaaatttca agaagacaga gcgcaggaaa gagacagaat
     4381 tctggataca gctcttgcgt ttggacttcg catctttttt gtggccatgc tgttgagtgc
     4441 gtttctggtg cgaaggattc aagaaatcat ttctggcgca cgacaggtag gagtcggaaa
     4501 tctggccgtc acatttgaaa cacgcgggtc tgatgaggta acggatctag ctcgttcact
     4561 caaccagatg gtggacggtc ttcgcgagcg cgaagcgatg cgaggcgaaa tgtccgctgc
     4621 agaagaaatc cagcgccgtc ttctcccaga caggatgccg gacaatatgg cggaagccct
     4681 ctcctttggc actttttata aagcaatgat gggggtgggc ggagattact ttgatctgat
     4741 ggaggcgggg cctgcgagta tggtgttctg catcgccgat gtttcaagcc acggatctgg
     4801 acctgcgatc gtaatgacca tgcttcggtc gtatcttcgc gcagccgtaa agcgcacgcg
     4861 cgacttgcga aaaattgtgt tggatttgaa cgcgagaatg tttgaggaaa ctccttctca
     4921 tatgttcatc actatgtttc taggactgta cgatcgggat aaacacacaa tcgaagctgt
     4981 gaactgcggg cataataagt ccttcatcta caaatacagt tctggcgcca tcgaggaatt
     5041 tgagagcacc tcgttgccac tgggagcagt ggattctgat ttgttcgcaa ctgcggtcac
     5101 cacgcagact attaaacttt cccagggaga tttgttcttt caatacacgg acggactcaa
     5161 tgaaggaatg aacgctgccg gagaagaatt tggacttctt cgagtgaagg acatcctgcg
     5221 cgaaaacggc cgcaagaagc cggtgattct tctggaaatg attgcgaaag cggcggagga
     5281 attcacaggc aagaagatct ttgtgcccgg gccatccgag cagaaagacg acattgcgat
     5341 gattgcgttt cgtcgcatga agtaagtttc cgcgggccgg gaatcgattg ttgtggcttg
     5401 acacattttt gggctcgtgt catcgaacgc cgtgcgacgc attctcctgg cactttcgat
     5461 tggcattcta tggtttggct ctccctcctg tcttagccga tgcattggag gagcgcacga
     5521 tttcaaagag ggcagcaagt actccagtct gagtccacgt gctcaggaac tcattcgcct
     5581 cgcgttttcg gatttggatg cgaagaagtt gatggactat catctccaca ttgtttctac
     5641 cggaaccact tgcaaagaat gctttatcaa tcctaacttt tattcatctc ttcatccaat
     5701 caaaagaatg cgtttcaaag tgtacgtttc tgccgctggc gtgacagatc tcactcgaac
     5761 ggacgagcag tatgtagaaa ggctccttgc tttgatcgac aacatggaag agcacggccg
     5821 atttttgatt cttgcttttg ataaacatta cacagagcag ggagaggtgg attcggaaag
     5881 tactgatttt tacgtatcca atgattatag catgcaggtt gcctctcgtt ccaggtttct
     5941 ggttcctgcg gtgtctgttc atccagcgcg aaaagatgcg ctcgtcgagc tagagagagt
     6001 ggcaaagaaa ggcgctcgcg tagtgaaatg gctgcccaat gcaatgggaa ttcatccgga
     6061 tgatccatcc tacaaaccct tctatgcgat gatgaagaag tacgatatga ttcttctctc
     6121 gcatgcagga gaagagcgtg cagtagacgc gcaggaatat caggagctgg ggaatcctct
     6181 ttatcttcgc gcgccactcg atgcaggagt caaagtgatt gttgcacatt gtgcgagcct
     6241 gggccaggcg caggatctgg atgcgcccgg tcatccgctc gtttccaatt ttgatttgtt
     6301 cgtgcgtcta ttcagcgaga aaaaatatac cgggcgatta ttcgctgaca tatctgcgat
     6361 gactcagttc aatcgtcttg gatctcatcc cgaaccggag cctttgaaat acattcttgc
     6421 tcatcctgaa atgcatgatc gcctcgtgaa cggaagtgat taccctctgc ctgcgatcaa
     6481 cattgtcatt cgaacaaagg cactcatgca gaacggattc atcagtaccg aggaaagaga
     6541 gagtctcaat gaaatctata aattcaatcc tcttctcttt gattttgtcg tgaagcgaac
     6601 tctgcaccat ccggagcatg ctgattggaa gctttctccc gggatttttc tcgaaaagaa
     6661 ggaattgagg ccgttccagt agagctggga tcggaaacga acacggaagt cctggcggtt
     6721 cggcaaatag gatgcctaaa aggcttgtcg caccgcgact ttagaattct ctgaatgcaa
     6781 gaaatcatgc tgaattctct tcaaatctat gaaaggctca ggcacgcgcg ggtggacacc
     6841 cgggcagctc gggaaatagc ttctgttttc ggggatctga tggatcacca gatcgccggc
     6901 aagcgcgatc tggcggagac cgaaacgcgg gtgaaagcgg aaatcaaagt ggagattcac
     6961 cgcgcaaaga gcgaaacttt gaaatggatg attttgtggg tttcgggact gcttgcggcc
     7021 caaaccggga tgatcatggc tgcaattcgc tttgttcgct agagttcgcc ggattttgct
     7081 ttccctgagg gccagggtcc agagattggc ccaaaccaga gagtaaagaa gagaaatgac
     7141 agagttacag gaaaaaaagg accgtacaaa gaccctgatc cgcgaattcg gcaagaacga
     7201 acgcgacacg ggttccactg aggtgcagat tgcactcctt tccgagcgca tcaaggcgct
     7261 caccgatcac tttaaaatca acactcggga ccatgcatcg cgcagagggc tcctgatgct
     7321 cgttggtcaa agaagacgcc tgcttgaata cctgaaaggc agggatcaag acaagtacaa
     7381 agatctaatc aacaagctcg gcctgcggaa ataatccagc cgagcttctc atcgccccgc
     7441 ctgtaactct cgggaggaaa atctatgttc aaagaagaag ccacaattga tggcaaaaaa
     7501 gtatcactcg aaaccggcaa ttgggccaaa caagcagacg gcgccattgt ttatcgcgca
     7561 ggcaacctgg tgttgcttgc cactgtctgc ggagaatcag aagcaaaaga aggccaggat
     7621 ttctttcctc tgactgttga atatcgcgag aagacgtatg cagccggaag atttccaggt
     7681 ggctacttca aacgcgaaac tcgtcctcaa gagcatgaaa cactcatctc tcgtatcatc
     7741 gatcgtccaa ttcgtccttt gttcccggaa ggattctttg cagaagttca agtattcgta
     7801 actgtacttt ctgcggatcc agacattgca gtagaaggcc acgcagtcac tgcggcgtct
     7861 gccgccattc acacttcaga cattccattt catggtccca ttgcagctgc agttgttggc
     7921 cgtgtagacg ggcagtttgt tgcagatccg ggtccttcac ttcttgcgaa gtcagacata
     7981 gagctgacag tggccggatc tctcactcac attgtgatga ttgaaggtgt gtgcgaagaa
     8041 tattccaagc aagacatcct ggatgcaatt gcatttggac acaagtctat caaagaaaag
     8101 atcaagctcc aggaagcaat ggcaaagaaa gtcaatcgca caaagcgcga tatcaagctt
     8161 cgcctgcctg atctggaact gaaaaaagaa gtctacgctt ttgctacaga caagatggct
     8221 gcggcaaaca gcacaaaaga caagctgaac agacagaacg aaatcgatgc agtcatcaaa
     8281 gataccaaag ctcatttcga agcaaagctt cgcgcagaag gaaaagacga aaagagcata
     8341 gccaattcac tcaaagaaat caaagaacat ctgtatttgc tcgaatatga agtcgttcgc
     8401 gacggcattt tcaaaaaagg aatccgttac gatggccgca agctcgatga gattcgcgat
     8461 atttccgttg aactcgatgt tctcccatct gttcacggat ctgcagtgtt tactcgcgga
     8521 cagacccagt ccctcggtgt tgtgactctg ggaacaggaa acgacgctca gaaatacgaa
     8581 aacctgagtg gcctccagag caaacagttc atgcttcatt acaactttcc gcctttctct
     8641 acaggcgaag tgaaacgcta ctcaggtcca ggccgccgcg aaatcggtca tggaaacctg
     8701 gcagagcgcg ctttgaaagt gatggtgcct aaacaggaaa cattcccgta cgtgattcgt
     8761 actgtatcgg aaatcctgga atcgaacggc tcctcttcta tggcatccgt atgctccgga
     8821 tctttagctt tgatggcagc gggtgttccc ataccgcgcg gtgtgtccgg catcgccatg
     8881 ggactcattc taggagataa agacgatttc gtagttctct ctgacattgc aggtctagaa
     8941 gatcactttg gcgatatgga cttcaaagtt gcgggaactg aaaaaggaat cactgcattc
     9001 cagctcgata tcaaactcac aggtgtgact ctggatattc tcgattctgc cattgagcag
     9061 gcaaccaagg gccgcgctca cattcttgga atcatgaaca aggctcttgc agaagcgcgc
     9121 tcgagcgttt ctgtcaatgc acctcgcatc acaaccatcc agattgatcc agaaagaatt
     9181 ggtgaactga ttggtccggg cggaaaagta atccgtgcga tcatcgaaaa atcaaaatca
     9241 gaaatcaatg tagaagacag cggaaccgtt accattgcat cttccggcgg agaagcaaac
     9301 gagtatgcca aaaagctcat catggatctc ttcgcagacg tggagccagg tgcgcaattc
     9361 aacggcactg taaaacgaat cgctgatttt ggtgcattca ttgaaatcct tccaggcaaa
     9421 gaaggactcc ttcacatttc caaaatggct cctactcgca ttaactctgt gcgcgacgtg
     9481 atgaacgaag gcgacatggt agaagtggta gtcacaggcg tagatcgtca gggcaaaatt
     9541 gaactgggta gaaaagacct ggtaggcgct ggaaacactc gtccttctcg ttatgatgaa
     9601 ggagaaggtg gtggcgatcg cggaggagac cgcggagatc gtgatcgcgg cggatatggc
     9661 ggagatcgcg gccatcgtgg tccacctcgc ggtggcgatc gcggacgcgg cggtcctcgt
     9721 ggcggcggcg gttcgagagg cggcggacgc gaccacaggt aatcgcctgt ggctcgcatt
     9781 cgcatcgcac tgaaacaggg cgcgcgcatt cccgcgcgtt ctacagacgg cgcggcaggc
     9841 tatgatgtct gcgcgcttct tccaccagac actgaaatca tcatcgcacc ggggcaaagg
     9901 atgctcgttc ctacaggcat tttcctggac attccagaag gctatttcgt gactcttcgc
     9961 ccgaggtccg gcctggcact gaagaatggg atcactctac tcaattctcc cgctaccatt
    10021 gactgggact accgaggaga actg
//
LOCUS       DAORFF010000052        10043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3091_length_10043_cov_3.449039, whole genome shotgun sequence.
ACCESSION   DAORFF010000052 DAORFF010000000
VERSION     DAORFF010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10043
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3091_length_10043_cov_3.449039"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PKK76_03530"
     CDS             <1..909
                     /locus_tag="PKK76_03530"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HNL67566.1"
                     /translation="APRHDYVQWIGSASNQGAGGLARNVGQVVGVFGTVDLSSLRPGY
                     TSSIQMTNLMQIESRLAQLQSPQWPASFPSLDKAKIARGREIFASTCMSCHADIRRDD
                     PKRKITAEMTSIELLGTDPLTAQNAVNAEADTGILKGRFVHVVGGVTRFGERSSVLDM
                     LRHVVEGLLAQQPDKIVKSSITNVGWFTTERHGQYERDAKNPVRSLLTYKARPLNGVW
                     ATAPFLHNGSVPTLYDLLLPADKRPATFKVGSNQYDPEKAGFVSDQGSFLFDTSLRGN
                     SNKGHEYGTSISEADRLALLEYLKSL"
     gene            complement(896..1915)
                     /locus_tag="PKK76_03535"
     CDS             complement(896..1915)
                     /locus_tag="PKK76_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL67567.1"
                     /translation="MRVHNYAAVPDPQKIGTDLYRIVIPQPFYEPNNIYLITSGEPTL
                     IDTGYIENLGLLQRSLRSIGHSLSKIKHIIYTHDHIDHMSAALSIRFYTDARTYAMAG
                     IEPHVANYASNLLLMKRAEERLTYKAHRDPSVRKAELARARKGWDEFTESYAGPGKVE
                     KAVRIDTGLMEGDVIECGSREIGFLYTPGHNRWHITPYIVGEGIYFTGDLVLDNVSSV
                     YAEVDGDLQKYHESLDRLFKIPIQRLLPAHGNEPEDPKKKIRLLQKTLALLERGVIRC
                     LKEKPHDLAELVSSAMGERVRKSGYYFTALGVMHSIIKKLAVQGQIVVHEIDPPYEQY
                     SLKET"
     gene            complement(1917..2396)
                     /locus_tag="PKK76_03540"
     CDS             complement(1917..2396)
                     /locus_tag="PKK76_03540"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNL67568.1"
                     /translation="MNVHDFTIKTITGEEKSLKEYSGKSLMIVNVASKCGLTPQYAGL
                     ESLYKKYKDRGFNILGLPCNQFLHQEPGTESDIQAFCSMNYGVTFDLGSKIEVKGKTP
                     HPLYAFLTGDGAKFPGKISWNFEKFLVGKNGEVLARFSPQTVPEDPSIVSAVESSLS"
     gene            complement(2398..5070)
                     /gene="acnA"
                     /locus_tag="PKK76_03545"
     CDS             complement(2398..5070)
                     /gene="acnA"
                     /locus_tag="PKK76_03545"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNL67569.1"
                     /translation="MSNSFGAKAKFTSKAGSLDLFRLDALEKQGVGNVSKLPYSIRVL
                     LESALRNEDSFQIHKEDVENLTKWDPTKQSDREFAFKPGRVVLQDFTGVPCVVDLAAL
                     RGAMVRMGGDPARINPIVRVDLVIDHSVQVDYFGSGDALKKNMAVEFERNRERYEFLK
                     WGQGAFQNLGIVPPGIGIVHQVNLEYLARGVLEKDGVAYPDSLVGTDSHTTMINGLGV
                     LGWGVGGIEAEVVMLGQPLYMLVPQVIGFKLKGKLREGATATDLVLRVTEMLRKKGVV
                     EKFVEFYGPGLSSLALADRATIANMAPEYGATTGFFPVDAETLRYLERTGRPPELIDL
                     VERYYKEQGMFRTDDSAEPVYTDSLELDLDSVEPALAGPKRPQDRVNLSEMKKTWQGA
                     LTKPVKERGFELKPDQINAKGHITGGYQAEITHGAVVIAAITSCTNTSNPGVMVGAGL
                     LAKKAVEKGLKVKPFVKTSLAPGSRVVTDYLDRAGLSQYLDQLGFQTVGYGCTTCIGN
                     SGPVPDPVVNAINEGNLVAASVLSGNRNFEGRISPNVKANFLASPPLVVAYALAGTVD
                     IDLSKDPIGTGKDGSAIYLKDIWPAQAEVKAAVDAITPEMYRKRYDDVRQANEMWNEI
                     PVSGGAVYKFNEKSTYIQEPPFFIDMPKDPPAQASDIKGARVLAKLGDSVTTDHISPA
                     GNIAASSPAGKYLVENGVTKDDFNSYGARRGNDRVMTRGTFANIRLRNLLIPDSEGNV
                     TRYLPTNEQMSIFDAAMKYKADKTPLVVLAGAEYGTGSSRDWAAKGTYLLGVRAAIAV
                     SYERIHRSNLVGMGVLPLQFLPGQSHESLGLTGEEVFEILGIAQAKPRGKLTVKAGEK
                     SFEVICRLDNPVEIEYYRHGGILQAVLRSFLKGK"
     gene            complement(5462..5728)
                     /locus_tag="PKK76_03550"
     CDS             complement(5462..5728)
                     /locus_tag="PKK76_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNL67570.1"
                     /translation="MREIHIRQMRYDDARAKLEKEIEEAFVAGETTVDIVHGIGGGVL
                     QKMAREYVNASDILREVPQFSNPGVLRAEILSPGKDTIKSWLRR"
     gene            complement(5728..6894)
                     /locus_tag="PKK76_03555"
     CDS             complement(5728..6894)
                     /locus_tag="PKK76_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67571.1"
                     /translation="MRSLFTLSLLIVSLCYAMTCSSQKDKTEVTEQGTTAGQEIASVV
                     SPTNADLDKSQNWNELAKLLAGVEGPKSDSPAVNRHRALMKDFWVRIQKENMEPIAAW
                     REKNLPESAKSRNVLYPLSGADFLNAYAFFPGASDYILIALEPPGSAPHLEKMTEAQR
                     ESGLASARKAIWTLAQNNYLQSRIMHDEFNNPNVTGTLPAFLIMLGGLGHEVKNVQDI
                     GIGKTGQLAAAADAPRTKGVLIQFKDSKDGKNKRLIYLSMRLEKQTAESTSPEGIFLR
                     GIGRRNTMMKSAVYILGWESMKLPHDVILGQSDLVIQDDSGIPYKSFQDGKWNEKLYG
                     TYVRALPVGGIPNPPQQGDLAAAYAKGASPLPFAYGYGILRGKGQSNLMLFTRR"
     gene            complement(6917..7909)
                     /locus_tag="PKK76_03560"
     CDS             complement(6917..7909)
                     /locus_tag="PKK76_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67572.1"
                     /translation="MPWWAGSMIQTLSVEILRFRQSFLGQIRAFFDSRGYLEVQTPAL
                     NPTGAVEPFIDSFQVHATGDRKSPAGPSGGTEGYLITSPEYNLKTVLAASPVPIYEIC
                     HSFRAGDVGAEHTEEFLMLEWYRPGATESDLILETNSLLVHLAGSFPLPSGLRIPATP
                     RTISIGDLFAREGIGGLGRDALAKAAVDMKLGSKADRYDELFFSLFLNRIEPSLRIPE
                     PVYLNGYPEELAALSNTERGLAKRFELYWNGLELANGYEELRNKEEHLRRFKEFNDIR
                     RKTGRPEMKGDPQFLENIEKMPECSGIALGVERLMMVFLGENHIANISPFAWPK"
     gene            8051..8971
                     /locus_tag="PKK76_03565"
     CDS             8051..8971
                     /locus_tag="PKK76_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNL67573.1"
                     /translation="MSAIHDTNKIQKEGREPRVVREKRKSGRDSEHGDSILNWYLNEI
                     HKIPMLDRETEFEVANLAKSGDRAARDRLVQANLRFVVTVARKYQSYGLTMMDLINEG
                     NMGLIRAAERFNPDRGYHFISYAVWWIKQAILFAIQQKAALIRLPLNRTADLRRIDEA
                     EKWIENHDDVENNLETLSGMLDMKSQEIQHLVNMSRDYLSLDAPMGDGEDFTLGDNIM
                     DQTEQVPESRVEGEELKREIEESLESLTSREKKVLQMRFGLQGSAVFSLQKIGKKVGL
                     SKERIRQIEKKAIRKIRTGTSGQKLLAYVN"
     gene            8991..>10043
                     /locus_tag="PKK76_03570"
     CDS             8991..>10043
                     /locus_tag="PKK76_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67574.1"
                     /translation="MRALQFTLAFLLILGPLLFRFPAREESFSPSINALYYYPFEGDP
                     SPLPNSWVKESVRPFNAVWIPNGDASRLILPNRRVDLNSEKMAFAPIHGDGYFIFQRV
                     GSEISFLSSLGELLWKKPFPAYPVTAPSGSPVFLMTGDANRVDVLDASGNLKGVKHIA
                     GNFLTDYKFSRSGRTCFVFGAGGLIVIDPQGEIAFKFESQDPAFYKSCALSPDGQVAA
                     VHALRGESDVVFVVRQKEKKASVSETLPLSAEYTHSLPLAVSNSGSVLVIPPEGALFF
                     KSGDLVWKSNEPAGLAYAFSDESFLAFANGTRTEVIDANGNRIARIPSPFGSGEPYQI
                     LPGPRKQQFLLQSYTGL"
BASE COUNT         2301 a         2563 c         2540 g         2639 t
ORIGIN      
        1 gcgccaaggc acgattatgt gcaatggatt ggaagtgctt ctaaccaggg cgcaggtggg
       61 cttgctcgta acgtagggca ggtggtaggc gtatttggaa ctgtggacct gagctctctt
      121 cgaccgggct atacttcatc catccagatg accaacctga tgcagattga atccagactg
      181 gcgcagctac agtctccgca gtggccggca agttttccgt cattggacaa agcaaaaatc
      241 gcgcgcggcc gagagatctt cgcgagtaca tgcatgagct gccacgcaga cattcgcagg
      301 gatgacccta aaagaaagat caccgcggag atgacttcca tcgagcttct gggtacagat
      361 ccattgaccg cgcagaatgc agtgaacgca gaagcagaca ctggaatact gaaaggtcga
      421 ttcgtgcacg tcgttggcgg agtgactcgt tttggagaaa gatcttctgt gctggacatg
      481 cttcgtcatg tcgtagaagg acttctggcg caacagccgg ataaaattgt aaagtccagc
      541 atcaccaatg ttggctggtt taccacggag cgccacggtc aatacgaacg cgatgcgaag
      601 aacccggttc gctcccttct aacctataaa gcgcgtccgc tcaacggcgt ctgggccacc
      661 gcaccatttc ttcacaacgg ttctgtccca acgttatacg atcttcttct tccggcggac
      721 aagaggcctg caaccttcaa ggttggctcg aatcagtatg atcccgagaa agccggattt
      781 gtttctgatc agggctcttt tttatttgat acttcccttc gtgggaattc aaacaaagga
      841 catgaatacg gcacgtctat cagcgaagca gacagactgg cacttctaga atatctcaag
      901 tctctttgag tgaatactgt tcgtaaggcg ggtctatctc atgaaccacg atctgaccct
      961 ggactgccag tttcttgatg atggaatgca tgactcccag agcggtgaaa tagtaaccac
     1021 ttttacgcac tctttctccc atcgcagatg atactagttc tgccagatca tgcggctttt
     1081 cctttaaaca gcgaatgacg cctctttcga gcaatgcaag agtcttctgc aacaagcgaa
     1141 ttttcttttt tggatcttcc ggctcattgc cgtgcgccgg caagagtctc tggattggaa
     1201 ttttgaaaag cctgtccagg gactcgtgat acttctggag atctccgtct acttccgcat
     1261 acacgctcga cacgttgtcc aggaccaggt ctcccgtgaa atagattcct tcccctacga
     1321 tgtaaggcgt gatgtgccag cgattgtgtc cgggagtgta tagaaaccca atctcgcgcg
     1381 aaccgcactc tattacatct ccttccatga ggcctgtgtc gattcgaact gccttttcca
     1441 ctttgcccgg accagcgtaa gactctgtga actcatccca tcctttcctt gcgcgtgcca
     1501 gttctgcttt tcgcacagaa ggatcacggt gcgctttgta cgtcaggcgc tcttctgctc
     1561 tcttcatcag gagaagattg gatgcgtagt tggctacatg aggctcaatt ccagccatag
     1621 cgtaagtccg cgcgtccgtg tagaagcgaa tgctgagtgc ggcgctcata tgatcaatgt
     1681 gatcatgggt gtagatgatg tgcttgattt tagaaagaga atgtccaatg gaacgaagtg
     1741 atcgttggag caggcccagg ttttcaatgt agcccgtatc aatgagagtg ggctctcctg
     1801 acgtgatcag atagatattg ttgggctcat agaaaggctg cgggatcacg attctataga
     1861 gatccgtccc gatcttctgt ggatcaggta ctgcagcgta attgtgtacg cgcatttcag
     1921 gaaagactgg attctactgc gcttacaata gaaggatctt cgggcacagt ctgcgggctg
     1981 aatcttgcga gaacttctcc gttcttgcca accaggaact tttcaaaatt ccaggaaatc
     2041 ttccctggaa atttggcgcc gtctccagtt agaaaagcgt ataacgggtg cggggttttt
     2101 cctttcactt caatcttgga tccaagatca aacgtgactc cgtagttcat gctgcagaat
     2161 gcctgtatgt ctgattctgt tccaggctcc tggtgaagaa actggttgca gggcaggccc
     2221 agtatgttga acccgcggtc tttgtatttc ttatacagag attcgagtcc agcatactgc
     2281 ggcgtaagcc cgcacttgct tgcgacattt acgatcatca ggcttttgcc cgaatactct
     2341 tttagagatt tttcttctcc ggtgattgtc ttgatcgtaa agtcgtgaac gttcatttta
     2401 ttttcctttc aggaagcttc tcagtactgc ctggagaatt ccaccatgac gatagtactc
     2461 aatttccaca ggattgtcca gtctgcaaat cacttcgaat gatttttcgc cagctttcac
     2521 agtgagcttt ccacgcggtt ttgcctgggc aattcccaga atctcaaata cttcttctcc
     2581 ggtgagtcca agggattcat gagactgacc aggcagaaac tgcagaggaa gcactcccat
     2641 acccaccagg ttggatctgt ggatcctttc atagcttaca gcgatggctg ctcgcactcc
     2701 caggagatac gttcctttgg cagcccagtc ccgcgaagat ccagtgccgt attctgcgcc
     2761 cgcaagaacc accagaggag tcttgtctgc tttgtatttc atcgcagcat caaagatact
     2821 catctgttcg tttgtgggta gatagcgcgt aacgtttcct tctgaatctg gaatgagcaa
     2881 attgcgaagg cgaatgttcg cgaaagtccc gcgtgtcatc acgcgatcat ttccgcgtct
     2941 ggccccataa gaattgaaat cgtctttggt cactccattc tccaccagat atttgccggc
     3001 aggagagctt gccgcaatgt tccccgcagg gctgatgtgg tctgtagtta cggaatcacc
     3061 cagtttcgca aggactcgcg ctcctttgat gtcggatgcc tgagctggag gatctttagg
     3121 catgtcgatg aagaacggag gttcctggat gtaggttgat ttttcgttga atttgtagac
     3181 tgctcctccg ctgaccggaa tttcattcca catttcattt gcctggcgaa cgtcgtcgta
     3241 ccgcttgcgg tacatttcag gagtgattgc gtctacggct gctttgacct ctgcctgggc
     3301 aggccagatg tccttgaggt agattgcaga accatctttt cctgtgccga ttggatcttt
     3361 ggatagatca atgtccacag ttcctgcgag agcatatgct acgacaagcg gaggacttgc
     3421 aaggaagttc gctttgacgt taggcgatat tcttccctca aagttccgat ttccagacaa
     3481 tacggacgcg gccacaagat ttccttcgtt gatcgcgttt accactggat ctggcacagg
     3541 accagagttt ccaatgcaag ttgtgcaacc atatccaacg gtctgaaatc caagctgatc
     3601 caggtactgc gaaagtcccg cgcgatccag atagtccgtc accacgcgag agcccggtgc
     3661 cagagatgtc ttcacaaatg gttttacttt cagccctttc tccactgctt tcttggcgag
     3721 tagtccggcc cctaccatca ctcctggatt ggaagtgttc gtgcaggatg tgatggccgc
     3781 gatgacgact gcgccatgtg tgatctctgc ctggtatcca ccggtgatgt gtcctttggc
     3841 gttgatctga tccggtttga gctcgaatcc tctttctttg acaggttttg taagagcacc
     3901 ctgccaggtt tttttcattt cgctcaggtt cactctgtcc tgaggacgct ttggtcctgc
     3961 gagtgctggc tccactgaat cgagatcgag ctccagactg tctgtataca ctggctccgc
     4021 tgaatcatct gtgcgaaaca ttccctgctc tttgtaatat ctttctacaa gatcaatcag
     4081 ttccggtgga cgacctgttc tttccagata gcggagagtc tctgcatcca ccgggaaaaa
     4141 tccagtggtg gctccgtatt ctggagccat gttcgcaatg gtcgcgcgat ccgccagagc
     4201 cagagaggaa agacccggtc cgtagaattc cacaaacttt tctaccacac ctttcttacg
     4261 gagcatttcc gtgacgcgca agacgagatc cgttgcagtg gctccttcgc gtagcttgcc
     4321 cttgagtttg aaaccaatca cttgaggaac cagcatatag agaggctggc cgagcatcac
     4381 aacttctgct tcaatcccac ctacacccca gccaagaact ccgaggccgt tgatcatagt
     4441 agtgtgtgag tctgttccca caaggctgtc tggataggcg actccgtctt tttccagtac
     4501 tccgcgcgcg aggtattcca ggttcacctg atgaacgatt ccgattccgg gaggaacaat
     4561 ccccagattt tgaaacgcac cctggcccca cttcaagaat tcatatctct ctcggtttct
     4621 ttcgaactcg actgccatgt tttttttcag agcgtcgcct gatccgaagt agtccacctg
     4681 cacagagtga tcgatgacca gatccactcg aacgatcgga ttgatccgcg caggatctcc
     4741 gcccatacgc accatcgcgc cgcgaagagc tgcgagatct acaacgcaag gaacgcctgt
     4801 gaaatcctgg agaaccactc tgcctggttt gaatgcgaat tcgcgatcgc tctgtttggt
     4861 gggatcccat ttagttaagt tttctacatc ttctttgtga atttgaaatg agtcttcatt
     4921 gcgaagtgca gactcaagta gaacgcgaat ggagtaggga agtttggaaa cgttgccgac
     4981 accctgtttt tcaagtgcgt cgagtcggaa tagatcgagt gatcctgctt tggaagtgaa
     5041 tttggctttt gcgccgaaag agttgctcat ggatcaacgt tcttttttcg aaaagcgcct
     5101 gtcaagcagg gaccagaacg tcgaatcgcg gttcgccggt cagccggacc ggcgaaacga
     5161 gcgcccctgc gccggcaggg ccggattttt ccgggctctg gccgggtagc cgctccagcc
     5221 agcgcgccat cggggcaaaa acagacacct gtccactgcc accagaaatc agcgccatgc
     5281 ccatgcaggc acggttttgc ccggtatcag cgtcaggccc tatgtttcca attcccatgc
     5341 gagctctagc tatcgtctgg atgcagccgg tctgccgatt ctaacgggta gcgtccctgg
     5401 cggcgccact ccaaacgatc attatttcag gcaggtaccg gggccagacg ttgaatgtcg
     5461 cctaacgtct gagccagctt ttgattgtat ctttgccggg gctcagtatt tccgctctga
     5521 gtactcctgg attcgaaaac tgcggaactt cccttaagat atcagaagcg tttacgtatt
     5581 cacgggccat tttttgcaga acgccgccac cgatcccgtg aacaatgtcc acggtggttt
     5641 cgcctgctac aaacgcttct tctatctcct tctccagctt cgcacgcgca tcgtcatagc
     5701 gcatttgccg gatatggatc tctcgcatta gcgtctggta aagagcatca ggttggactg
     5761 cccctttcct cgcagaattc cgtaaccgta tgcgaagggc agggggcttg cccccttcgc
     5821 gtacgcggct gccaggtctc cttgctgagg aggattagga atgccgccta caggaagagc
     5881 gcgcacgtaa gtgccgtata atttctcatt ccattttccg tcctggaaag atttgtatgg
     5941 aattccagaa tcatcctgaa tcacaagatc gctctggccc aggatcacgt catgaggaag
     6001 tttcatgctt tcccagccca ggatgtagac tgcagatttc atcattgtat ttcttcttcc
     6061 gattccgcgc agaaagattc cctcggggct tgtgctttct gcggtttgct tttcaagacg
     6121 catggacaag tagatgaggc gtttgttttt tccgtctttg ctatctttga actgaatcag
     6181 cactcccttc gtgcgcggag catctgcggc agcagcgagc tggcctgttt ttccaattcc
     6241 aatgtcctgt acatttttta cttcatggcc aaggccgccg agcatgatga gaaaagcagg
     6301 cagagttcca gttacgtttg gattgttgaa ctcatcgtgc atgattctgc tttgcagata
     6361 gttgttttgt gccagagtcc agattgcttt tcgcgcagat gcaagtcctg attctctctg
     6421 tgcttctgtc atcttttcca gatgaggcgc gcttccaggg ggttcgagtg caatgagtat
     6481 gtaatcactt gcacctggaa aaaaagcgta cgcgttgagg aagtcagcgc ccgaaagagg
     6541 gtagagtaca tttctcgatt tcgcagattc cggcagattc ttttctctcc acgctgcgat
     6601 aggctccatg ttttctttct ggatacgaac ccagaaatcc ttcatcagcg ccctgtggcg
     6661 atttacagcg gggctgtcgc tctttgggcc ttctactccg gcgaggagtt ttgcaagctc
     6721 attccagttt tgagatttgt ccaggtccgc attcgtggga gatacaacag acgcaatttc
     6781 ctggccggct gtagtcccct gttcagtaac ttctgtcttg tccttctgag aagaacaggt
     6841 catcgcatag caaagtgaaa cgatgaggag gctgagtgtg aaaagtgagc gcatatggtt
     6901 cattatcggt ttgttgttat ttgggccagg caaaaggact gatgttcgca atgtggttct
     6961 ctccaagaaa taccatcatc agacgttcga cgcccagggc aattccggaa cattcaggca
     7021 ttttttcaat attttcaagg aactgcggat cgcctttcat ttcaggtctt cctgttttcc
     7081 tgcgaatgtc gttgaattcc ttgaaacgtc tcaggtgttc ttctttgttt ctcaattctt
     7141 cgtaaccgtt ggccagttcg agcccattcc agtagagttc gaatctcttt gccagacccc
     7201 gttccgtatt gctgagagct gcgagttctt ccggatatcc gtttagatag acaggttctg
     7261 gaattcgaag cgatggctcg atacgattga gaaacaaaga aaagaagagt tcgtcgtatc
     7321 gatccgcctt tgatcccagt ttcatgtcca cggcggcttt ggccagggcg tcccgcccca
     7381 gtcctccgat tccctcccgg gcaaatagat cgcctatgga aatagttcgg ggtgttgctg
     7441 gaattcgaag accagacgga agcggaaagg aacctgccag atggacgagc agggagtttg
     7501 tctccagaat caggtctgat tctgtcgcac ctggcctgta ccattccagc atgaggaatt
     7561 cttccgtatg ctctgcaccc acatctccag cccgaaaaga gtgacagatc tcgtagattg
     7621 gcacgggact tgccgccagg accgttttga ggttgtattc cgggctcgtg atcaggtagc
     7681 cttctgtgcc ccccgatggg ccggcggggc tttttcgatc gcccgtagcg tgcacctgga
     7741 agctgtcgat gaatggttcc acggctccgg tggggttcag tgccggggtt tggacctcca
     7801 gatagcccct ggaatcaaaa aatgcccgga tttggccgag gaaggactgc ctgaatcgta
     7861 gaatttcaac ggaaagagtc tgaatcatgg aacctgccca ccagggcatg ggtctaagag
     7921 gcaattcccg atcagggagt caaaaaaaag cgaagtttaa acgattaaaa tggttgatca
     7981 gggcgagtag atgccgtatt ttagaatagt tctaaatctg aagggctttt gatcttgagg
     8041 tgaggacaga atgagcgcaa tacacgatac aaataagata caaaaagagg gccgtgaacc
     8101 cagggtcgtt cgagaaaaac gcaaatccgg gcgcgactca gagcatgggg attccatcct
     8161 caactggtac ctgaacgaaa tccacaagat tccgatgctg gaccgggaga ctgagttcga
     8221 agtggccaac ctcgcaaaaa gcggagacag agcagccaga gacaggctgg tccaggcaaa
     8281 ccttcgcttt gttgtgactg tagcccgcaa ataccagtct tatggtctca ccatgatgga
     8341 cctcatcaac gaagggaaca tgggcctcat tcgcgcagcg gagaggttca acccggaccg
     8401 cggctatcat ttcatctcgt acgccgtatg gtggatcaaa caggccattc tgtttgccat
     8461 ccagcaaaag gcagcgttga ttcgccttcc cctgaatcgt acagcggacc ttcgtagaat
     8521 cgatgaagcg gaaaagtgga tcgaaaacca cgacgacgtg gagaacaatc tggaaactct
     8581 ttctggaatg ctcgacatga aatcgcagga gatccagcac ctggtgaaca tgagccgtga
     8641 ttatctgagc ctggatgctc ctatgggaga cggcgaagat ttcactctcg gcgataacat
     8701 catggatcag acagagcagg tccctgagag ccgcgtggaa ggcgaagaac tgaagcgcga
     8761 aattgaagag agcctcgaga gtctgacttc tcgcgagaag aaagtactgc agatgagatt
     8821 tggtctgcag ggaagtgcag tattctctct gcagaagatt ggcaaaaagg tcgggctgtc
     8881 caaagagcgc attcgccaga ttgagaaaaa agcaatccgc aaaattcgca ctggaaccag
     8941 cggccaaaaa ctacttgctt acgtgaattg aaacagaaga gctctcttca gtgagagctc
     9001 ttcaatttac actcgcattc ctattgattc tcgggcctct tttgtttcgc tttccggccc
     9061 gggaagagtc tttttcccca tccatcaacg cactctacta ctatccgttc gaaggagatc
     9121 cgtctcctct ccctaattcc tgggtcaaag aatccgttcg acctttcaat gcggtctgga
     9181 ttccgaatgg agatgcatcc aggctcattc ttcccaatcg tcgcgtggat ttgaattccg
     9241 agaagatggc atttgctccc atccacggag acggctattt tatttttcag agagtaggat
     9301 ctgagatcag cttcctgagt tccctcggag aacttctatg gaagaaacca tttcctgcgt
     9361 atcctgtgac tgctccctca ggttcgcctg tattcctgat gaccggagat gcaaaccggg
     9421 tagacgttct cgacgcgagc ggcaacctca aaggagtcaa acacatagcc gggaactttc
     9481 taaccgatta caaattctcc agatcaggta ggacctgctt tgtatttggc gccggaggat
     9541 tgatcgtaat tgatccgcag ggagaaattg cgttcaagtt tgaatcccag gatccagcgt
     9601 tctacaaaag ctgcgccctt tctccagatg gacaggtggc tgcggtgcat gctttgcgcg
     9661 gggaatcaga tgttgtattt gtggtgcgac agaaggaaaa aaaggcctcc gtttcagaga
     9721 ctcttcctct ttccgcagag tacactcatt cacttcctct tgcggtctcc aattctggat
     9781 cggttctggt gattcctcct gaaggagctc tgtttttcaa gtcaggggat ctggtctgga
     9841 aaagcaatga acctgccgga cttgcgtacg cgttttccga tgaatctttt ctggcctttg
     9901 caaatggaac gcgaacagaa gtgatcgatg caaatggaaa tagaatcgct cgcattcctt
     9961 ctccgtttgg atccggggag ccttaccaga tattgcctgg gcctcgcaag cagcagtttc
    10021 ttcttcagtc ttacactgga ctg
//
LOCUS       DAORFF010000053         9950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3131_length_9950_cov_3.990197, whole genome shotgun sequence.
ACCESSION   DAORFF010000053 DAORFF010000000
VERSION     DAORFF010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3131_length_9950_cov_3.990197"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PKK76_03575"
     CDS             <1..265
                     /locus_tag="PKK76_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNL67575.1"
                     /translation="QSKIDPGGPADFNLYDATPEQKAKLKSVPPNLTKVLDALEADHS
                     WLTKGGVFDEDYISNYIEYKRAEVEKIVNQRPHPYEFVLYYDC"
     gene            complement(353..763)
                     /locus_tag="PKK76_03580"
     CDS             complement(353..763)
                     /locus_tag="PKK76_03580"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNL67576.1"
                     /translation="MIITPIQTRWNDMDPFAHVNNANYVAYLEIGRVDYCVRRLGVTE
                     LYAVPFLLARIEVDMIRPIEFGAGVEVLTCVSKIGNKSWDFLAFIRDSKSKEDFARAR
                     TVQVAYDHRRKQSQVIPESLRSILHEDWKSYQEQ"
     gene            complement(907..2031)
                     /locus_tag="PKK76_03585"
     CDS             complement(907..2031)
                     /locus_tag="PKK76_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNL67577.1"
                     /translation="MKKVFVMGLVLCMSGLFTAANATVRKTANPVVFAHGLAGWDNLL
                     GYFYFGDGVGNFVGDACDEFLEVVCNGGLNSSQKAFAAGVQPFASSEVRGTQLADQIQ
                     SYLATVGATYVNIIGHSQGGIDARKAAVVLKQRKGFTVVKTMVSVSSPHRGSPVAKYI
                     LNLGPGVTSVVSALATFYGNTVYGAGNDPIAAAKQLVYNDYSSTDGVTTGMKAYNTAY
                     ASYSTAVSNRLASVITAQQGLDVNAALYILSKGFYDIDGDGASCAVASDDCDNDGALG
                     KGNGSKTDEDDDGLVGINSQQMGYRLAYSTCFLCDDYMTQDTGLGYLTDMNNPTSAQQ
                     SSHADVVHQDHLDVTGAGTTTLDRDEFYAAIIDYMAANGG"
     gene            2448..3497
                     /locus_tag="PKK76_03590"
     CDS             2448..3497
                     /locus_tag="PKK76_03590"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HNL67578.1"
                     /translation="MQKRGSSSNPESRFIHTKREMEESDLDPDAVSSPSTELFADKSR
                     TVLSTNDSPDVPFSQSVNPYRGCEHGCSYCYARPTHEYLDLSAGIDFESKIFVKYDAA
                     KLLKEEISKKSYKPDVICFSGVTDSYQPIERKLQITRACLSVLREFRNPVSIITKNHL
                     ITRDVDILGEMAKDNLSCVLVSVTTLDENIRSRMEPRASTAARRLETIRFLSDHGIPV
                     GVLVAPVIPGLTDHEIPAILKVVKEAGASFAGYVMLRLPHGLKELFLEWLGTNFPNQK
                     KKVTDRILDIRGGTLYDSSFGTRGRGEGAFAEQIQSLFRLSLKKFALNSVGPKLRTDL
                     FEPVQGLLFPDPNKS"
     gene            complement(3505..5430)
                     /locus_tag="PKK76_03595"
     CDS             complement(3505..5430)
                     /locus_tag="PKK76_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL67579.1"
                     /translation="MCLPHGSAAFASVVVTVAVSRNKLLARIRTVFIPRRPDIRFFSE
                     ELHYQSSRVAGFGAFLLLFVCLPYLWLDPLIHPGSPWMLVLRIGQSLMGLIALVAVFF
                     DRVRKNAMYLVFVNLLYVELSGAVIAGLTGGDPVYTGGFALVLMITCLVPVPRMYALL
                     TAFASVAVYAWFMVSAKLDVSIPRNIFALQLIVTSLSITVFFIFILDRIRHSSFEKTL
                     AIQAARDDILKLSEFAQKANQSIDLNKILDEIIAYVQENFSIEGTLLQLYDPKTDELV
                     YFKTNFQNFHDEDLRKYVQALRFSAGKRSSAGAILRRGKPMYFPNMKVARRNENQDPL
                     DHFVVRAGIESALIVPLIVADAPAGMLYFSNYHGDMNLSRDQLSTIYAFCQQIGGAIH
                     SSSLIHQVEREKEKSERLLLNILPESVALELKENGFVRPTMFENVTILFTDFKGFTHH
                     AASMSPQDLVTELDGIFGQFDAIAEKYGLEKLKTIGDSYMCVGGLPKSNRTHPIDACL
                     AGLEMAAFVKQANELRLASTGSAFWEIRVGVHTGSVVAGVIGKSKFAYDIWGDAVNVA
                     SRMESGGEPGRLNISGDTAEYVRTFFDLEPRGEVAVKNRGTIPMYFVKGLRTGLQDSN
                     GRPGPGFHDLYDRLLIA"
     gene            5481..7004
                     /locus_tag="PKK76_03600"
     CDS             5481..7004
                     /locus_tag="PKK76_03600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNL67580.1"
                     /translation="MDENRYQELLSCIETLESWAGNPDDFTQLPEEVRVRLIRVAGQI
                     ARPNRLEQKNRNKALRRAKRSAIVAQERSARAATGIRDARRATVFKAPTQIEGPSHDS
                     APELSSPRNCYVCNAEYTKLHFFYDSMCQTCGDFNYQKRFQTAPLGDQVALITGARLK
                     IGYQAALMMLRAGARVIVTTRFPVDAANRYSKEDDFASWSDRLQIFGLDLRHTPSVEL
                     FAQYFEQTYDRLDILINNAAQTVRRPPGFYAHLMENETAGLSSIDEKARTLLSAHEDC
                     LRHLNLFSETNLATEKALPVTWNGRLPGIGLTSSARLSQIPYTHDQSLVAEQVFPAGK
                     LDADLQQVDLRETNSWRLRIGEIPTAEMIELQLINAIAPFVLCNRLAPMMQKNPTGQK
                     HIVNVTAIEGKFYRFRKPDRHPHTNMAKAALNMLTHTSASGLAKHGIFMNAVDTGWVT
                     DEDPAILAKHKQDVHDFEPPLDIVDGAARVCDPFFHGILTGQHWCGKFLKDYQPIDW"
     gene            complement(7046..9157)
                     /locus_tag="PKK76_03605"
     CDS             complement(7046..9157)
                     /locus_tag="PKK76_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNL67581.1"
                     /translation="MGKNSNTIESLFGQNVFNQHVMKTMLPKETYRAFKRVLEQGQPL
                     TAAQADDIAAAMKDWAVEKGATHFTHWFQPMTGFTAEKHDSFIKPTDDGRAMAEFSGK
                     ELIKGEPDASSFPSGGLRATFEARGYTAWDISSPAFLKEDEGGLTLCIPTAFCSYTGE
                     ALDKKTPLLRSMDALSREAVRVLRLIGNTQVQKVTTTVGAEQEYFLIDREYYEQRPDL
                     IFAGRTLFGAMPPKGQEMEDHYFGSIKDRIAGYMRDLDRELWKLGVSAKTKHNEVAPA
                     QHELAPIFQTTNIAADHNQLTMEMMRKVAIRHDLVCLLHEKPFAGVNGSGKHNNWSMS
                     TDDGQNLLDPGHTPHENVQFLLFLSAVIKAVDEYGDLLRVSAANPGNDHRLGANEAPP
                     AIISVYLGDQLGHILDQIDAGDAKESKRGGILDSGTPMLSTLPKDSSDRNRTSPFAFT
                     GNKFEFRMVPASISISAPNFTLNTIVAEALSGIGDRLEKATDRRAEVKTIIQEIVRKH
                     KRVVFNGNNYAKEWVAEAAKRGLPNISSTVEAFKSIVAEKNVALYEKHKVFTRTELEA
                     RYEISLENYSKTIRIEALTMVDMARRMIQPSVIRYATFLAESVQTVKAAGADVSVQEG
                     LLKSVSSLGVQFNEKLGALEKAIEKADATHGSSQAHAEAYRDVVFTAMGALREVGDKL
                     ETIVDESFWPMPTYGDLLFRV"
     gene            complement(9213..>9950)
                     /locus_tag="PKK76_03610"
     CDS             complement(9213..>9950)
                     /locus_tag="PKK76_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNL67582.1"
                     /translation="FLAHGASVCILGRDEEKLSRAAADLKASGNVFTVSCNLRSDEEI
                     PRAMDELASALGGLDILVNNAGGQFPGGTEQISAKGFRAVVDTNLTGTFLITKACFER
                     FLKKSGGSIVSILMDMRNGMPLMPHSAAARAGVDNLTKSWAAEWAHFGVRVNSVAPGI
                     IRSSGLDTYAPEFRKIVEEMALKNYAYRAGTEEEVSAAVLFLSSSLASYITGQTLFVD
                     GGESIYSPFLPPRPHDRLPAYLQNKRN"
BASE COUNT         2524 a         2586 c         2422 g         2418 t
ORIGIN      
        1 ccagagcaag atcgatccag gtggaccggc agacttcaac ctctatgacg caacgcccga
       61 acaaaaagcg aaattgaaat cagttccgcc taacctcaca aaggttctcg acgcactgga
      121 agcggaccac agctggctga ccaaaggcgg agtattcgac gaagattaca tcagcaacta
      181 catcgaatac aagcgcgcgg aagttgaaaa aatcgtaaac cagagaccac acccttacga
      241 atttgttctc tactacgact gctgatattc agctgcttta ccgggaagaa gcgtccgcga
      301 aagcgggcgc tttttttttg cagagagggg cttccgcgcg gactgttatt ccttattgct
      361 cctggtagga tttccaatct tcgtgcagaa tggaacgaag ggattccgga atcacctgcg
      421 attgtttgcg gcgatgatca tacgctacct gcacggttct tgcccttgca aaatcttcct
      481 tcgactttga atcccgaatg aatgccagaa aatcccagga cttgtttcca attttggaaa
      541 cgcacgtcag cacttctacg cctgcaccaa attcaatcgg acgaatcata tccacttcga
      601 ttcgagcaag cagaaatggt acagcataca attcagtcac tcccagccgt cgcacacagt
      661 agtccacccg gccgatttcg aggtaagcca cgtagttcgc gttgtttacg tgagcaaaag
      721 gatccatatc gttccagcga gtctggatcg gagtgatgat cattcttact cgatgtggac
      781 agcgtgcgag cggtcaattg tttccctcag atgttggtaa cgaattgccg aggcggcgga
      841 aggcgcagac ggaggcaaaa aaaaacccgg ccgcgaagcc gggtcctttc gattcagtga
      901 atacaatcag ccgccgtttg cagccatgta gtcaatgatc gccgcgtaga attcgtcgcg
      961 gtctagagta gttgttccag cgccggtcac atccaggtga tcctggtgaa ccacatctgc
     1021 atgagaactt tgctgagcag aagtaggatt gttcatgtcc gtcaggtaac caaggccagt
     1081 gtcctgagtc atgtagtcat cacaaaggaa gcaagtggag tacgccagac gatatcccat
     1141 ctgctgagag ttgatgccta ccagtccgtc atcatcttcg tcagttttgc ttccgtttcc
     1201 ttttccgaga gctccgtcgt tgtcgcagtc atcagaagca actgcacagg atgcaccgtc
     1261 gccgtcgata tcatagaagc ccttgctcaa aatgtagaga gcggcattca cgtccaggcc
     1321 ttgctgggct gtgatcacag atgcaagacg attggataca gctgtggagt agctcgcata
     1381 cgctgtattg taagctttca ttccagtcgt tacgccatct gtggagctat agtcattgta
     1441 aaccagttgc ttggctgctg cgatcggatc gtttcccgcc ccatacacag tgtttccgta
     1501 gaacgtggcc agtgcgctca ctacgcttgt aacacctgga ccaagattca ggatgtactt
     1561 tgcaaccggt gagccgcgat gaggagaaga aacggaaacc attgttttga cgacagtgaa
     1621 accttttctt tgtttcagaa ctacagccgc tttgcgcgca tcgatgccgc cctgggaatg
     1681 accaatgatg ttcacatagg tagctcctac tgttgcaagg tagctttgaa tttgatccgc
     1741 gagctgagtg ccacgaactt ctgagctagc aaatggttga acgcctgcgg caaacgcctt
     1801 ctggctggaa ttgagtccgc cgttgcaaac cacttccagg aactcgtcgc atgcatcacc
     1861 aacgaagtta ccgactccat caccaaagta gaaatacccg agcaggttgt cccatcctgc
     1921 cagaccgtgt gcgaaaacga ctggatttgc cgttttgcga accgttgcat tcgctgctgt
     1981 aaacaagcca ctcatgcaga gcacgagccc catcacaaaa acctttttca tttttcccct
     2041 tcgcgcgcac gaaggcgcac ttttgatgac tccactcttg catagagtgt accaggtacg
     2101 cgaaagcagt ttttcgcacg gcctcttttg tatttcgtat ctactgattt cgtgcatcgc
     2161 accagtgatt tcgctttcgc acgcgcaagg acgtttcgcg tctaaaaatc ctgtagcggt
     2221 gcactgcgat ttttcgcatg gatcgcgcgc gtgcaaaagc gttttcgcac atgaactgag
     2281 agaagcatca tttcttattt cctggattcc aatttatagg aacgttccag ttttcaggaa
     2341 atcactaatt ctcgggaacg accgaactaa tccgaaggga ctagaaacgt aaacacaatc
     2401 gtcgtccaag ggcattgaca attgcgcaga agaattgatt ctgatccgtg caaaagcgtg
     2461 gaagttcttc gaatccggaa agcagattca tccacacgaa acgcgaaatg gaggaatcag
     2521 accttgatcc agacgcagtc tcttctccat ctaccgagct tttcgcagac aagagccgca
     2581 cggttctaag cacaaacgac agcccggacg ttcccttctc gcaaagcgta aatccataca
     2641 ggggttgcga acacggctgc tcctactgct acgcgcggcc tactcatgag tatctggact
     2701 tatctgctgg aattgacttt gaatccaaaa tctttgtaaa atacgacgcc gcaaaactgc
     2761 tcaaagagga aatctcaaaa aagtcataca aaccagatgt aatctgtttc tcgggagtta
     2821 cggattctta ccaaccaatc gaaaggaaac tccaaatcac gcgagcctgc ctttctgttc
     2881 tgcgcgagtt tcgcaacccc gtatccatca ttactaagaa tcacctgatc acgcgggatg
     2941 tagatatcct gggcgaaatg gctaaagaca acttgagctg tgtgctggta tctgtgacta
     3001 ctctcgatga aaacatacga tccagaatgg aacccagggc atccacagcc gcgcgaaggc
     3061 tcgaaaccat ccgcttcttg agcgatcatg gaatccccgt aggtgttctt gtagcaccgg
     3121 tcatcccggg cctgacggac cacgaaattc cagccatact caaggtagtg aaagaggcag
     3181 gcgcgagttt cgcaggctac gtgatgctac gattgcctca tgggctcaag gaactttttc
     3241 ttgaatggct cgggacgaat tttccgaatc aaaaaaagaa agtaacagac agaatcctgg
     3301 acattcgcgg tggaactctc tatgattcga gctttggaac ccgaggtcgg ggagaaggcg
     3361 ctttcgcaga acaaatccag agtctatttc gcctgtctct caagaaattc gctttgaatt
     3421 cggtcgggcc aaaacttcgc acggatctgt ttgaaccggt gcagggactt ctctttcccg
     3481 accctaataa atcatgaaag aacgttaggc gatcagtaac ctgtcgtaca gatcgtgaaa
     3541 gccggggccg ggtcgcccat tggagtcttg caaacctgtg cgcagtccct tcacaaaata
     3601 cattggaatg gtaccgcgat tcttgacagc cacttcacct cttggttcaa gatcaaaaaa
     3661 cgtgcgaacg tactctgccg tgtcccctga aatattcaat cgaccgggct caccgcccga
     3721 ctccatgcgc gacgccacat tcacggcgtc tccccagatg tcgtaggcga atttactttt
     3781 acctatcaca cccgccacca cagatccagt atgaacgccc actcgaatct cccagaatgc
     3841 ggaccctgta ctggccaggc gaagttcatt tgcctgcttc acaaacgccg ccatttctaa
     3901 tcctgcgagg cacgcatcaa tgggatgagt tcgattcgat tttggaaggc cgcccacgca
     3961 catgtaggaa tcgcctatgg tcttgagctt ttcgaggccg tacttttctg cgatcgcatc
     4021 aaactgtcca aaaatcccat ccaattccgt taccagatcc tgaggtgaca tagatgctgc
     4081 gtgatgagtg aatcctttga aatcggtaaa cagaatggtt acgttctcga acatggttgg
     4141 gcgaacgaaa ccgttctcct tcaactcaag agccacgctc tcgggaagaa tattcaacaa
     4201 tagcctttcc gatttttctt tttcacgttc cacctggtga atcagtgaac tggagtgaat
     4261 cgcgcccccg atctgctgac agaaagcata aatagtactg agctggtccc gggataaatt
     4321 catatctcca tgatagttag aaaaatatag cattcccgcg ggtgcatccg caacgatcaa
     4381 aggcacgatc aaagcagact cgattccagc gcgcaccacg aagtggtcca gggggtcctg
     4441 gttttcattt cttcgcgcaa ctttcatgtt gggaaaatac attggttttc cgcggcgaag
     4501 gatggctccg gccgagcttc tctttccggc agaaaaacgg agcgcctgca catactttcg
     4561 cagatcttcg tcatgaaaat tttgaaagtt cgtcttgaaa tacactagtt catctgtttt
     4621 gggatcgtac aactgaagga gcgtcccttc aatggaaaag ttctcctgga cataggcaat
     4681 gatctcatcc aggattttgt tcaaatcaat gctttgattt gccttctgtg caaactcaga
     4741 cagcttcagg atgtcatcgc gcgccgcttg tattgctaga gttttttcga aactcgaatg
     4801 gcgaattcta tccaggatga aaataaaaaa cacagtaatg gaaagagagg taacgatcag
     4861 ctgcagcgcg aaaatatttc gcgggataga cacatctagc ttcgcagaga ccatgaacca
     4921 ggcgtaaacg gcaacactgg caaaggctgt taagagagca tacatgcgtg gcacgggaac
     4981 caggcatgta atcataagaa ccagagcgaa tcctccggtg taaactggat ctccgccggt
     5041 gaggccagca atcactgctc cggataattc cacgtagagt agatttacga atacgaggta
     5101 catcgcattc ttacgcacgc gatcaaagaa aactgcaaca agtgcaatca gtcccatcaa
     5161 actctggcca attcgcaaaa caagcatcca cggagaacca ggatgaatga gcggatcgag
     5221 ccacaggtaa gggaggcaca cgaacaagag caagaacgca ccgaacccgg ccacccggct
     5281 ggactggtaa tgcaattctt cgctgaaaaa acgaatatcc ggcctgcgcg ggatgaatac
     5341 ggtgcgaatg cgcgccagta gtttgtttct gcttacagcc acagtcacga ctacggatgc
     5401 aaaggccgcg cttccatgag gcaagcacaa tcgttccttt tttcagttga accgcagggc
     5461 agcgcctgct ttcgatccat atggatgaga atcgctatca ggaactactg agttgcatag
     5521 agactctgga aagttgggcc ggaaatccag acgatttcac acaacttcct gaagaagtaa
     5581 gagtgcgtct cattcgtgtg gcaggacaaa tcgccagacc aaacaggctg gagcaaaaga
     5641 atagaaacaa agctctgcgt cgggcaaaac gatctgccat agtggcacag gaaagatccg
     5701 cgagagccgc cactggcatc cgggacgcgc gtagagccac ggtgttcaaa gctcccacgc
     5761 agatagaagg gccctctcat gactccgcgc cagaactgag ttcaccccgc aattgctacg
     5821 tatgcaatgc cgaatacacg aagcttcact tcttttacga ttccatgtgt caaacctgtg
     5881 gcgatttcaa ctatcagaag agatttcaaa ccgcaccgtt aggcgatcag gtggcactca
     5941 ttactggcgc acgattgaag attggatatc aggccgcgct gatgatgctt cgcgcaggcg
     6001 ctcgcgtaat tgtcactacc cgcttccccg tcgacgcagc aaatcgatac agcaaggaag
     6061 atgattttgc atcctggtcg gatcgcttgc agattttcgg tctggactta cgtcacacac
     6121 cgagcgttga gctcttcgct caatactttg agcaaacgta tgatcggctg gacatactga
     6181 tcaacaacgc cgcgcaaacc gtgagacgtc ctccgggatt ctacgcacat ctgatggaaa
     6241 atgaaaccgc cgggctttct tctatcgacg agaaggcgcg caccctgctt tctgcgcacg
     6301 aagattgttt gcgtcatttg aatctatttt cagagaccaa tcttgccact gagaaggctc
     6361 ttcccgtcac ctggaacgga agactcccgg gtataggact cacttcttct gccaggcttt
     6421 cacagatacc ctacactcac gatcagtccc ttgtagcgga gcaggtgttt ccggccggca
     6481 aactcgatgc tgatttacaa caggtagatc ttcgcgaaac caacagctgg cgtcttcgca
     6541 tcggtgaaat cccaaccgcg gaaatgatcg agcttcaatt gatcaacgca atcgctccct
     6601 ttgtactgtg caaccgcctg gctcctatga tgcagaaaaa tcctacaggg caaaaacaca
     6661 tagtgaacgt gactgcgatc gaaggaaaat tttatcggtt caggaaacca gaccgtcatc
     6721 cgcacacgaa catggcaaaa gcagcactca acatgctgac ccacacttca gcaagcggat
     6781 tggccaaaca tggtattttc atgaacgcag tagacacagg atgggtcacg gatgaggatc
     6841 ctgctatcct cgcgaaacac aaacaggacg tacacgattt tgaaccacct ctggatattg
     6901 tggatggagc cgctcgagtc tgtgatccat tcttccacgg aatcctgact ggccagcact
     6961 ggtgtgggaa atttcttaaa gattatcaac caatcgattg gtagatgcga cggccagaac
     7021 ggccgtcgca cattgtctca ctttgttaaa cgcggaagag aaggtctccg taggttggca
     7081 ttggccagaa ggattcatcg acgattgttt cgagtttgtc tccaacctca cgcagagcgc
     7141 ccatggctgt gaaaacaacg tcacgatagg cttccgcgtg cgcttgcgaa gacccgtgcg
     7201 ttgcgtctgc cttttcgatg gccttttcca gagccccgag cttctcattg aactgcacgc
     7261 ccagagaaga tacagacttg agcagtcctt cttgaacgga tacgtcagcg ccggccgctt
     7321 ttacagtctg aactgattca gcaaggaatg tcgcataacg gatgacggaa ggctgaatca
     7381 tgcgacgagc catgtctacc attgtcagag cttcgatgcg aatggtcttg ctgtagtttt
     7441 ctagactgat ttcgtagcgc gcttccagtt ccgtgcgagt gaatactttg tgtttctcat
     7501 agagagccac gttcttctct gcgacgatgg acttgaaagc ttctacggtg ctggagatgt
     7561 ttggaagacc gcgttttgct gcctcggcaa cccattcctt cgcatagttg tttccgttga
     7621 aaaccacacg tttgtgctta cgcacaattt cctgaatgat cgttttgact tctgcacgac
     7681 ggtccgttgc tttttccaga cgatctccaa tcccagaaag cgcttccgct acaattgtgt
     7741 tgagcgtaaa gtttggagca gaaatagaaa tcgatgctgg caccattctg aattcgaatt
     7801 tgtttccagt gaatgcaaac ggtgatgtac ggtttctgtc agaagaatct ttgggcagtg
     7861 tagaaagcat tggagttccg ctgtccagga ttccgcctct cttggattct ttcgcatctc
     7921 ccgcgtcgat ctggtccagg atgtgtccca gctgatctcc gagataaaca gagatgatcg
     7981 caggaggagc ttcatttgca ccaaggcgat gatcgtttcc cgggttagcc gcggagacgc
     8041 gaaggagatc tccatactca tccactgctt tgatcacagc gctcaagaac agcaggaact
     8101 gtacattttc gtgtggtgta tgacctggat cgagaaggtt ctggccgtca tctgtagaca
     8161 tggaccagtt gttgtgcttc ccggaaccgt tgacgcccgc gaatggtttt tcgtgcagca
     8221 gacacacaag gtcgtggcga atcgcaactt tgcgcatcat ttccattgtg agctgattgt
     8281 gatctgcggc aatgttggtg gtctggaaga tgggtgccag ttcgtgctgt gcaggtgcta
     8341 cttcgttgtg ctttgttttt gctgaaacgc ccagcttcca caattcacga tccagatcgc
     8401 gcatgtagcc cgcgatgcga tccttgatac ttccaaagta atgatcttcc atttcctggc
     8461 ctttcggagg catggctcca aaaagtgtac ggccagcaaa gataaggtca ggacgctgtt
     8521 cgtagtattc gcggtcgatg aggaagtatt cctgttccgc tcccacagtt gtggttactt
     8581 tctgcacctg agtgttgcca atcagacgaa gcacacgaac cgcttctcgc gaaagagcat
     8641 ccatggagcg aagcagtgga gttttcttgt ccagagcctc tccagtataa gaacagaatg
     8701 ctgtgggaat gcagagcgtg agtccacctt cgtcttcttt gaggaaagca ggcgagctga
     8761 tgtcccatgc ggtgtaacca cgtgcttcga acgtagcgcg aagaccgcct gacgggaagg
     8821 aagaagcgtc tggctctcct ttgatcagtt cttttcctga aaactccgcc atagctctac
     8881 cgtcatcggt tggcttgatg aatgaatcat gtttttctgc tgtaaagcca gtcattggct
     8941 ggaaccagtg agtgaagtgt gttgcgcctt tttcaactgc ccaatctttc attgcggctg
     9001 caatgtcatc cgcttgagct gcagtcagag gttgaccctg ctcgagaacg cgtttgaatg
     9061 cgcgatatgt ttctttgggc agcattgtct tcattacatg ctgattaaat acgttctgtc
     9121 cgaataacga ttcgattgtg tttgaattct ttcccacagg ggtctccgaa gtctttttgc
     9181 cagaggacca caatttacga ggcggtcaaa tcttagtttc tcttattttg caagtatgct
     9241 ggaaggcgat catgcggccg tggcggcaag aaaggcgaat aaatggattc tccgccgtcc
     9301 acgaacaacg tctgccccgt gatgtaggag gcaagtgacg aggagaggaa caaaacagcc
     9361 gcgcttactt cttcttccgt gcctgcgcga tacgcgtaat tcttaagagc catctcctcc
     9421 actattttgc gaaattcagg tgcgtacgta tccagaccac tcgatcgaat gatgccgggt
     9481 gcaacagaat tcacgcgcac gccaaaatga gcccactcgg cagcccacga ttttgtaaga
     9541 ttgtccactc ctgcgcgcgc cgccgcggag tgcggcatca gcggcattcc atttctcata
     9601 tccatgagaa tgctcacgat cgatccaccg gactttttca agaatcgctc aaagcaggcc
     9661 ttcgtaatca agaatgtacc cgtcaggttt gtatctacga ctgcgcgaaa tcctttcgca
     9721 gaaatctgtt ccgtccctcc aggaaattgc ccgcctgcat tgttcaccag aatgtcaagc
     9781 ccaccgagcg cgcttgcaag ttcgtccatc gcgcggggaa tttcttcatc cgatctcaaa
     9841 ttgcaactca cggtgaaaac atttccactg gctttcaaat cagcggcagc acgggacagc
     9901 ttttcttcgt ctcgcccgag aatgcaaaca gaggctccgt gcgcgaggaa
//
LOCUS       DAORFF010000054         9915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3151_length_9915_cov_4.213895, whole genome shotgun sequence.
ACCESSION   DAORFF010000054 DAORFF010000000
VERSION     DAORFF010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9915
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3151_length_9915_cov_4.213895"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKK76_03615"
     CDS             <1..294
                     /locus_tag="PKK76_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNL67583.1"
                     /translation="MPYIITEPCQGVCDTACVDVCPVDCIHPRKDEWDKKGYSKDNLG
                     GKQLFIDPQECIDCGACEPACPVQAIFPEDEVPEKWKSFIGANYSHYGHTYKG"
     gene            complement(363..1109)
                     /locus_tag="PKK76_03620"
     CDS             complement(363..1109)
                     /locus_tag="PKK76_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNL67584.1"
                     /translation="MDEPMGLAAIALILFLGSTGVTAFASGFLFLPAKTQKSLLPILV
                     SFATGALLATALLVMIPDTLRQAGTQKTMQWVLIGILFFFLLERLVIFRHCHEDECST
                     HRAAGAVILTGDAIHSFFDGVIIASAFQYSVELGIATSISILIHEIPQEISDFAVLIQ
                     SGYTRNRALLMNYLAALPSVAGGILTFYFLSELRHLLPFFMSISAASLIYVAIADLVP
                     GLHRYAGLRTGLFHFLFLLAGVGVIAWIRG"
     gene            1181..1265
                     /locus_tag="PKK76_03625"
     tRNA            1181..1265
                     /locus_tag="PKK76_03625"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1215..1217,aa:Ser,seq:tga)
     gene            1273..1361
                     /locus_tag="PKK76_03630"
     tRNA            1273..1361
                     /locus_tag="PKK76_03630"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1307..1309,aa:Ser,seq:gct)
     gene            1387..1800
                     /locus_tag="PKK76_03635"
     CDS             1387..1800
                     /locus_tag="PKK76_03635"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNL67585.1"
                     /translation="MADKIREIQSRFSRNMQNNDSVAAVIGYIPLFGWIYPLFFRKEN
                     DFCQFHGKQAMHLNAGMLVVYFAVWILENFPITSWLFGADSWFHPITQAAWRITMLGY
                     FGLCAFGAYKAFIEERWEIPFLSDFVDRIQNQIKK"
     gene            1804..2181
                     /locus_tag="PKK76_03640"
     CDS             1804..2181
                     /locus_tag="PKK76_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67586.1"
                     /translation="MRARILSIPLHIRRVLYTGPFFVLWVIGYTVETSATELRAAGRG
                     LVTLIGFVASHAVILFVRELIRAFTVPAYPVEFTFFLLKSLIGLGYLGLTVWIAVREY
                     RNQETTLPFIDRLALRIDSVLSR"
     gene            2183..3244
                     /gene="corA"
                     /locus_tag="PKK76_03645"
     CDS             2183..3244
                     /gene="corA"
                     /locus_tag="PKK76_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNL67587.1"
                     /translation="MARLYRRDVARKRGAPPGTLVYTGDEKASTEIQLIEYDEERFQE
                     EVIKDPKVFQAYKDDPAVTWINVRGLSDVGPVVKMGEVFGLHPLVLEDILNTDQRPKF
                     EDYEKYLYIVFKNVTWDAKGEANYEQISLILGEDFLISFTESENDILKSLRDRLRTAK
                     GRVRRMGADYLAYCVMDLAVDNYFTVLEATGERLEILEESLLDPAANPSVREIQRMKR
                     MLLMMRKSVWPLREVVMKMERRESGLFKPGTEYFLRDLYDHTIQVVDTLETFRDMLQG
                     LMDIYLSTVSNRLNVVMKTLTVITTIFMPLTFLAGIYGMNFKHMPELEWTYGYPALIA
                     AMGMIAGTMYYWFRKRDWL"
     gene            complement(3245..4333)
                     /locus_tag="PKK76_03650"
     CDS             complement(3245..4333)
                     /locus_tag="PKK76_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNL67588.1"
                     /translation="MSLLYDARLVENSGIGTTIRGQLREILSRKIPITLTGNSRQLAS
                     HSLTGVPVIPFDAKLYSIKEQTSFPRASHARIHVPHYNAPLRYLSHCSVTVHDLIHLQ
                     SDEFKHPAYRAYASLMLSQIARRSPCILTVSETTRSQFLSRYPEARHRTFTTLNGIDH
                     KLFRPASSGAVAALKKKHSLPDEYFLAIGIGKKHKNIDFALRALKHLWKDTDCPPIVL
                     AGAGGKIPSYATHAMMENSVRKNVILLPHVPESDLPAVYSSALALIMPSLLEGFGLPA
                     LEAMACGTPVLASTGGSLPEVCGHAARFFDPRDEDAFIHAVQVVLQDSKQRARLRSLG
                     LSQAAGFTWKRNVDAMMRAYHHTGFEDS"
     gene            complement(4343..5347)
                     /gene="ilvC"
                     /locus_tag="PKK76_03655"
     CDS             complement(4343..5347)
                     /gene="ilvC"
                     /locus_tag="PKK76_03655"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNL67589.1"
                     /translation="MAQVFYDDSCDSKFLKGKTIAVLGYGSQGHAQAQNMRDSGLNVI
                     IGLKADSKSAPIAREDGFEVLSPFDAAAKADIIQLLAPDQVQMTIYEESIKPNLKKGK
                     ALVFSHGFNIHYELIKPPADVDVYMVAPKGPGHLVRRVYREGGGVPCLIAVHQDATGE
                     AKQRALAHASGVGGGRAGILETNFKNETETDLFGEQVVLCGGLTSLILAGFETLTEAG
                     YDPDIAYFECLHEVKLITDLIYEGGIARMRHSISDTAEYGDLTRGPRIVDAGVKARMK
                     EVLTEIQKDKGAKFATEWLAESKNGYPNFNKLREAGLKHPIEQVGQKLRGMMKFLGKK
                     "
     gene            5431..6474
                     /locus_tag="PKK76_03660"
     CDS             5431..6474
                     /locus_tag="PKK76_03660"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNL67590.1"
                     /translation="MKIFITGGAGFLGYHLVQRLKKDKHKITLYDIADFNPADYPKDV
                     KMIKGDVRDAKALQEAMKGHDAVIHAAAALPLWKKEDIYSVTIDGTRNVLDSAAVNKI
                     DRVVYISSTAVYGIPKKHPILETDPRVGVGPYGEAKIEAEKICEEYRHRTKMCICVIR
                     PKTFIGTGRLGVFQILFDWVESGVKIPVFGNGKNRYQLLDVRDLCDAIELGMTGNAKK
                     ANDNFNVGAEKFQTVREDVGALCAFSKSGSRVFGVPSSPLKIALRFFEILHISPLYRW
                     VYGTADKDSFVSTEKLTKALGWHSKYSNSDALIDSFRWYLVNKPQDADSVAGVTHRVP
                     WKQGILKVFKKFL"
     gene            complement(6486..7643)
                     /locus_tag="PKK76_03665"
     CDS             complement(6486..7643)
                     /locus_tag="PKK76_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNL67591.1"
                     /translation="MFEWVHYQFPARIFIEKNATHKIGTLVKEVGSRALLVALKSEMS
                     NPDELAVIKTSLDKYTSGCILYDDMASRPGNKELDTAAHFAKQSRADIIIGYGGRDSF
                     HAAKAIALLCKNDVFAEDLPHTALPLKRAPLPVAQVPAAPSMGEETSPVFSVYNPETR
                     SAFFSQDYRLFPAIIFVDPNITSTLSKGELSRAGAANVAAAVESILSRRANDITTSIA
                     LRSLDLVQKNLQQVMKKPGNMAARTNVSLASVLCGMAHSNSMLGLCYSIASATSLLTG
                     LEFHLAMALLLPHVMEYNLTTSAGRYVQIARALDEDIKDITVIEAAIKAVEGVRKTYL
                     ELKIPQRLSEFEIAKEDLPEIAELAFRIPMVKNSPRDLDKNEIETILIAAY"
     gene            7793..9205
                     /gene="rsmG"
                     /locus_tag="PKK76_03670"
     CDS             7793..9205
                     /gene="rsmG"
                     /locus_tag="PKK76_03670"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNL67592.1"
                     /translation="MALSASRLADDLADAGIELDSHQAKQLLRFYELVEERNPDLNMT
                     RIRGYEATVRKHYVDSLIILRYLKRIFPPLGLPASILDFGTGAGFPGVPLAIACPETR
                     VILNESRANRAEFLEDVVEECGLKNATVLGKRLAMEDDIHVDAVIARAVSTMADLLTR
                     TIFSIKKGGSIIYMKGPNCDAEIAEMGAGVSLLLDQSYTLPASEDHRRLVCFRVDDPG
                     EWKAPGSNAISSRENEKFKMLISLQSSRGIRKNGLCLIGGKKIVSEVVSNARVRVQAA
                     VVPQSFSGSMPPDVRQFEFKNDLFAQIDELGTGAPLLLAEIPEMSEWDGRVKGLCVVI
                     PFQDPENVGAAIRSAVAFGAGRIILTKECANPFLARAIRSSAGAVFVAPLVKGPSLVE
                     LLENYPEIYQLSSDGIALEDVNFSKDILITAGLEGPGLPGAGGNRLRIVMRSDVESLN
                     AATALAIALYEFDRSQRRKT"
     gene            complement(9187..>9915)
                     /locus_tag="PKK76_03675"
     CDS             complement(9187..>9915)
                     /locus_tag="PKK76_03675"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL67593.1"
                     /translation="GIVAASQSAKQVLVFVHGFNVKFEEAVYRAAQIKYDLRFKGPVV
                     LFTWPAGSDDGILSSLNVSGTYEMNFQNARKSRNQFREFITALSGGGSTVHLIVHSMG
                     HQVVIPALTEGGPPPRLGEVIFNAPDFALEDFKKAAPILRPAVKRITLYCSPGDNALV
                     ASEKVNGGKRIGRCAKIPDVDVVNVNEVDSPVLGIGGLGHGYYSGRAILTDVYQVLLG
                     VDVSRRLFVRKSSEYGGEDYVLRR"
BASE COUNT         2433 a         2296 c         2629 g         2557 t
ORIGIN      
        1 atgccataca ttatcacaga accctgccag ggcgtgtgcg atacagcatg cgtagatgtc
       61 tgcccggttg attgcattca cccacgtaaa gacgaatggg acaagaaagg ctattccaaa
      121 gacaacctcg gcggaaagca gctgttcatc gatccgcagg agtgcattga ttgtggagcc
      181 tgcgaacccg cctgcccggt gcaggcaatt tttcctgaag acgaagttcc ggaaaagtgg
      241 aagtcattca ttggtgcgaa ctattctcat tacggccata cttacaaggg ctaaagagag
      301 tgtcttggca atgccggcgt agaatccggc attgcttctg attttcgcgc cccctctttc
      361 catcaaccgc gaatccaggc aatcacgccc acgcctgcga gcaaaaacag aaagtgaaat
      421 aatcccgttc tcaatcccgc ataacggtga agtcccggca ccaggtccgc gatggctaca
      481 tagatcaagc tcgcagctga gatcgacatg aagaatggaa gtagatgcct gagttcagat
      541 agaaaataga atgtgagaat tccacctgcc acagacggaa gggccgccag gtagttcatc
      601 aaaagagcgc gatttcttgt atatccgctc tggatcagca ctgcgaaatc gctgatttcc
      661 tgcggaatct catgaattag tatggagatg ctcgttgcga ttccaagctc aaccgaatac
      721 tgaaaagcgg aagcgatgat cacgccgtca aagaagctgt gaatcgcatc tccagtcagg
      781 atgaccgctc ccgctgcgcg atgagtggag cactcgtctt catggcagtg gcggaagatt
      841 acaagtctct cgaggaggaa gaagaacaaa atgccgatca aaacccactg catcgttttt
      901 tgcgtaccag cctgacgcaa agtgtctgga atcatcacca ggagtgctgt cgcaagtagc
      961 gcgcccgtgg cgaaactgac gaggatgggc agaagcgatt tctgggtctt cgcaggtaga
     1021 aatagaaagc ctgaagcgaa cgcagtgacg cctgtcgaac caagaaacaa aatgagcgca
     1081 atggcagcga ggcccatggg ttcatccatt tgttgcatga gttgatattt tgaaagtctt
     1141 tctagttgac actcgcctga aaagtaccag cctggaaagt ggagggatgg ccgagtggtc
     1201 gatggcggcg gtcttgaaaa ccgttgtgca gagatgtacc gtgggttcga atcccactcc
     1261 ctccggggaa ttggagaggt ggctgagtgg ccgaaagcgg cagtttgcta aactgttgta
     1321 cgagttaaat cgtaccgcgg gttcgaatcc cgccctctcc gaaatttcga ggccttccag
     1381 gaagtcatgg ctgacaagat tagagaaatt caatcgcggt tttcgcgaaa catgcagaac
     1441 aatgattctg tcgcagcggt gatcggatac attccgctct ttggttggat ctatccgctc
     1501 ttttttcgca aggaaaatga tttttgccag tttcacggta agcaggcaat gcatttgaat
     1561 gcaggcatgc tggttgttta cttcgcagtc tggattctcg aaaattttcc cattacgtcg
     1621 tggttattcg gcgcagattc atggtttcat ccaatcacgc aggcggcctg gcgtattact
     1681 atgctcggat atttcgggct ctgtgcattt ggcgcataca aagctttcat cgaagagaga
     1741 tgggagatcc cgtttttgag cgattttgtg gatcgcatcc agaaccagat taagaagtaa
     1801 atcatgcgcg ctcgaattct tagcattcct ttgcacatcc gtcgagtcct ttacacaggt
     1861 cctttttttg tgctctgggt aattggctac acagtggaaa ccagtgcgac agaactcagg
     1921 gcagccgggc gtggcctggt aacattgatt ggttttgttg cttcgcacgc agtgattcta
     1981 tttgtgcgag agctcatcag ggctttcact gttccggcct atcctgttga atttacattc
     2041 tttctactga aatccctgat cggcctcggg tatctgggac tcactgtctg gattgcagta
     2101 cgcgaataca gaaatcaaga aaccactttg ccattcatag acaggcttgc gcttcgcatc
     2161 gattccgttc tgagcaggta gtatggctcg gctttataga cgagatgtag cccggaaaag
     2221 aggagctcct cccggaactc tagtctatac cggggatgaa aaagcttcta ctgagattca
     2281 actcatcgag tatgatgagg aaaggtttca agaagaagtc attaaagatc ctaaagtatt
     2341 tcaagcgtac aaggatgatc ctgccgtcac ctggatcaat gttcgtggtt tgtcggatgt
     2401 gggcccggtt gtaaaaatgg gtgaggtgtt tgggctgcat ccactggtgc tggaagacat
     2461 tctgaatacg gatcaaaggc ccaaattcga agactacgaa aagtatctct atattgtgtt
     2521 taagaacgtg acgtgggatg cgaaaggtga agcgaattac gagcaaatca gcctgatttt
     2581 aggggaagat ttcctcattt ctttcacaga atccgagaat gatattctaa agtcgcttcg
     2641 agatcggctt cgcacagcca aaggccgcgt gagacgaatg ggtgcagact atctggctta
     2701 ttgcgtcatg gatcttgccg tggataacta tttcacggtg cttgaagcca caggcgagag
     2761 actggaaata cttgaagaga gtcttctgga tcctgcggca aacccatccg tgagagaaat
     2821 ccaaagaatg aagcgaatgc ttctgatgat gcgcaagagt gtctggcctt tgcgcgaagt
     2881 agtcatgaag atggagcgtc gcgaatccgg tctattcaag cctggcacgg aatactttct
     2941 tcgtgatctc tatgatcaca ccattcaggt tgtggataca ctggagacat ttcgcgacat
     3001 gttgcaaggc ttgatggata tctatctttc tactgtgagc aatcgtctca acgtagtgat
     3061 gaagactctc actgtgatta caaccatctt catgcctctc acgttccttg cgggaattta
     3121 cggaatgaat ttcaaacata tgccagagct ggaatggacg tatggctacc ctgcattgat
     3181 cgcagcgatg gggatgattg caggcacaat gtattactgg tttagaaaaa gagactggct
     3241 ctagtcagga atcttcaaat ccggtgtgat gatacgctcg catcatcgcg tctacatttc
     3301 gcttccacgt aaatccagcg gcctgcgata aaccaaggga tcgtagtcgt gcgcgctgtt
     3361 tggaatcttg caacacaacc tgcactgcgt ggatgaatgc gtcttcgtcc cgcggatcaa
     3421 agaagcgcgc tgcatgaccg catacttccg gaagacttcc gccagtggat gcaagaactg
     3481 gagttccaca ggccattgct tctagagcag gcagtccgaa tccttcaagc agagagggca
     3541 tgatgagcgc cagagcagaa gagtatacgg caggaagatc tgactcagga acatgaggga
     3601 gaaggattac attttttctg accgagttct ccatcatggc atgtgtcgcg taagatggga
     3661 tttttcctcc ggctcccgcg agcacgatgg gcggacagtc cgtgtctttc cagaggtgct
     3721 tgagcgcacg cagggcgaag tcaatattct tatgcttctt tccaatgccg attgccagaa
     3781 agtattcatc ggggagagaa tgtttctttt tgagtgcagc gaccgcaccg gagctggccg
     3841 gcctgaatag tttgtggtca atgccgttta acgttgtgaa tgtgcggtgc cgtgcttctg
     3901 gatatcgact gaggaactgg cttcgcgttg tttcagaaac agttagaatg cagggtgaac
     3961 ggcgtgcgat ttgcgaaagc atgagcgacg catatgctct gtaagccggg tgcttgaatt
     4021 cgtctgactg aagatggatc aagtcatgga cagtcacaga acaatgagac agataacgaa
     4081 gtggtgcgtt gtaatgagga acatggatgc gtgcgtgcga agctctgggg aaggatgttt
     4141 gctcctttat ggaatatagt ttcgcgtcaa agggaatgac ggggacgccg gtgagcgaat
     4201 gagatgcaag ttgtctgctg tttccggtaa gagtgatggg gattttgcgg gaaagaattt
     4261 cccgcaattg accccggatc gtggttccga tgccggagtt ctcaacgagt ctggcatcgt
     4321 ataacaggct catggccgga tgttattttt tgcccaggaa tttcatcatt ccgcggagct
     4381 tttgacctac ctgttcaatt gggtgtttca ggcctgcttc gcgaagtttg ttgaagttcg
     4441 ggtaaccatt cttggattct gcaagccatt ccgtcgcgaa tttcgcgcct ttatctttct
     4501 ggatttcagt gagtacttct ttcatacgag ctttcactcc agcatccacg attcgtggtc
     4561 cgcgcgtgag gtctccgtat tctgcagtgt cagagataga atggcgcatg cgtgcaattc
     4621 caccttcgta gatcaggtcc gtgatgagtt ttacttcatg cagacattca aagtaagcaa
     4681 tatccggatc gtaaccggct tctgtcagtg tttcaaaacc ggcgaggatg agtgatgtga
     4741 gtcccccgca aagaactact tgttcaccga agagatctgt ttccgtttca ttcttgaaat
     4801 ttgtttccag aattccagcg cgacctccac caacaccgga cgcatgcgcg agagctcttt
     4861 gtttggcctc tcctgtcgca tcctggtgaa ctgcaatgag gcaaggaact cctccgcctt
     4921 cgcgatacac gcgacgcacg aggtgaccgg gacctttggg agcgaccatg taaacgtcaa
     4981 cgtctgccgg tggtttgatg agttcgtagt gaatgttgaa gccatgcgaa aacacgagtg
     5041 ctttcccttt cttcagattg ggtttgatgc tctcttcgta gattgtcatt tgcacttgat
     5101 ccggtgcaag caactggatt atgtccgctt tagcggctgc gtcaaatggt gaaagtactt
     5161 cgaaaccatc ttcgcgggcg ataggtgctg atttggagtc tgctttgagg ccaatgatga
     5221 cattcaggcc tgaatcgcgc atgttctgcg cttgtgcatg tccctgactg ccataaccaa
     5281 gaacagcgat tgtttttcct ttaaggaact tagaatcgca agaatcgtcg taaaaaacct
     5341 gtgccatgcg gacacaacgc gttcaggtca gtgcgatggc aaggaaaaac tttacagggg
     5401 ggcctctcaa aaaaaattaa ccaaaatact atgaaaatct ttattaccgg cggtgccggc
     5461 tttctgggct atcaccttgt acagagactg aagaaagaca aacacaagat cactctttat
     5521 gatatcgcag attttaaccc cgctgactat ccaaaagatg tgaagatgat caaaggggat
     5581 gtgcgcgatg cgaaggcatt gcaggaagca atgaaaggac atgatgcagt cattcacgct
     5641 gctgccgcgc ttcctctctg gaaaaaggaa gatatctatt ctgtaacgat agacggcacg
     5701 cgaaacgttc tggatagcgc tgctgttaac aagatcgatc gagtagtgta tatcagctca
     5761 actgcagttt atggtattcc caagaaacat ccaattcttg agactgatcc tcgtgtgggc
     5821 gtaggccctt acggagaagc aaagatcgaa gcggagaaaa tttgcgaaga gtatcgtcat
     5881 cgcacaaaga tgtgtatttg tgttattcgc ccaaaaacat tcattggaac aggccgtctt
     5941 ggtgtgtttc agatcctttt tgactgggtc gaatccggtg taaaaattcc tgtctttgga
     6001 aacggaaaga accgttatca acttcttgat gtaagagatc tctgtgatgc gatcgaactt
     6061 ggaatgaccg gaaatgctaa aaaggccaac gacaacttca atgttggtgc ggaaaaattc
     6121 cagacagtgc gcgaagacgt cggcgctctg tgtgcctttt caaaaagcgg ctcgagagtc
     6181 tttggagtcc catccagtcc cctcaagatc gctcttagat tttttgagat ccttcacatt
     6241 tctccgcttt atcgctgggt atacggaacg gcagacaagg attcattcgt ctccactgag
     6301 aaactcacga aagctcttgg atggcattcg aagtattcga attcagacgc gctcattgat
     6361 tctttccggt ggtatcttgt gaacaagccg caggatgcag atagcgtggc aggtgttacg
     6421 catcgcgtac cgtggaagca agggatcctg aaagtattca agaaatttct ttaagaatga
     6481 atggcctagt aggccgcgat caggattgtt tcaatttcat tcttgtctag atcccgtggt
     6541 gagttcttca ccatgggaat ccggaacgca agttccgcaa tttcgggcaa atcctctttt
     6601 gcaatttcaa attcgctcag tctctgagga attttcaatt ccaggtaggt tttgcggacg
     6661 ccttccaccg ctttaatcgc agcttcaatc acggtgatgt ccttgatgtc ttcgtcaagc
     6721 gctcgcgcaa tttgcacata acgccccgca gacgttgtaa gattgtattc cattacgtgc
     6781 ggaagtagaa gtgccatagc cagatgaaat tccaggcctg taagtaggct cgtcgcggat
     6841 gcaatgctat agcagagacc gagcatactg ttcgaatgcg ccattccgca aagcacgctt
     6901 gcaagactca cgttggtgcg tgcagccata ttgccaggtt ttttcattac ctgttgcaag
     6961 tttttttgca caagatccag agagcgcaga gcaatcgacg ttgtgatatc gttagcacgt
     7021 cgagacaata tcgattccac ggcagcggcg acgtttgccg caccagctcg actgagttcg
     7081 cctttgctca gtgtgcttgt gatatttgga tcaacgaata tgatcgcggg gaacagtctg
     7141 taatcctgtg aaaagaatgc ggatctagtt tctggattgt aaactgagaa cacaggactc
     7201 gtttcttcgc ccatggaggg cgccgcagga acctgggcga ctggcagagg cgcccgtttc
     7261 agtggaagtg cagtgtgcgg tagatcttct gcgaagacat cattcttgca caggagagca
     7321 attgcctttg cagcgtgaaa ggagtctctg ccgccataac cgatgatgat gtcggctctc
     7381 gattgcttcg cgaaatgggc cgcagtgtcg agttccttat ttccaggccg cgaagccata
     7441 tcgtcgtaga ggatgcagcc cgacgtatat ttgtccagac tggttttgat gactgccagt
     7501 tcatctggat tcgacatttc gcttttcagc gcgaccagaa gcgctcgact tcccacttct
     7561 tttacgaggg tgccgatctt gtgggtcgca ttcttttcaa taaaaatgcg agcaggaaac
     7621 tgatagtgta cccattcaaa cacaggccgc ttcctcgcag ttggatagtg acagggatgg
     7681 attgttcaaa atggacgatg ggctcccgct atagtatcgg aggttttcgg ttaatccctc
     7741 gctcatttcg cgctcctgct tgacctgtgc attcaggaat gtgcggtgaa ctatggcgct
     7801 cagtgcatcc aggctcgcag acgatctggc agatgccggg atcgaactgg attctcacca
     7861 ggcgaagcag ctgcttcgct tttacgaact ggtcgaggag cgcaacccag acctgaacat
     7921 gacgcggatt cgcggatatg aggctacagt acgcaagcac tatgtggaca gcctgatcat
     7981 tcttcgatat ctaaagagaa tttttccacc ccttggattg ccggcgtcca tacttgattt
     8041 tggtacaggc gccggatttc cgggagttcc gctggcgatc gcctgtcctg aaaccagagt
     8101 cattctgaac gaaagcagag ccaatcgcgc ggagttcctc gaggatgtgg tagaagaatg
     8161 cggattgaag aacgcaacgg ttctgggtaa gcggctcgcc atggaagatg acattcacgt
     8221 ggacgcagtc atcgcacgcg cggtgagcac aatggcagat ctcctgactc ggaccatctt
     8281 ctccatcaag aagggcggtt ccatcattta catgaaaggt ccgaactgcg atgcagaaat
     8341 tgcggagatg ggtgcgggtg tgtctcttct tctcgatcag tcatacaccc tgcctgcttc
     8401 ggaagatcat cgtcgcctgg tgtgctttcg cgtggatgat ccaggagagt ggaaagctcc
     8461 tggatcgaac gcaatttcat cccgagaaaa cgaaaagttc aagatgttga tctctctgca
     8521 gagttcccgg ggtatcagaa agaatggact ttgtcttatt ggcggaaaga agattgtttc
     8581 ggaagtagtg tccaatgcgc gcgtgcgagt acaggcggcg gtggttcctc agagcttttc
     8641 cgggagcatg ccgccggacg taaggcaatt tgagttcaag aatgatctat tcgcacagat
     8701 cgatgagttg ggtacaggtg ccccgttgct tcttgcggag attcccgaaa tgtccgaatg
     8761 ggatggaagg gtgaaaggtc tttgcgttgt gattcccttt caagatccgg aaaatgtagg
     8821 tgctgccatc aggagcgcgg ttgcctttgg tgcgggcagg atcattctca caaaggaatg
     8881 tgcgaatcct tttcttgcgc gggccattcg ttcctctgcg ggagcagttt ttgtggcccc
     8941 actggtgaag ggaccttctc ttgtggaatt gcttgagaac tatccggaaa tctatcaact
     9001 ttcgtctgac ggaatcgccc tcgaagatgt gaatttcagc aaggatattc tgatcacagc
     9061 gggtctggaa ggcccgggtt tacccggagc tggcggcaat cgtctacgca tcgttatgcg
     9121 atcagatgtg gaatcactga atgccgcgac agctcttgca attgcgcttt atgagtttga
     9181 cagaagtcag cgtcgcaaaa cgtaatcttc gcctccgtat tcgctacttt tgcgcacaaa
     9241 gagtcgccgg ctaacgtcca ctccgagtag tacctgatat acgtccgtga gaatcgcacg
     9301 acctgagtag tacccgtgtc cgagtcctcc gattcctaga acgggagaat ctacttcgtt
     9361 tacgtttaca acatctacat ctggaatctt cgcacatctg ccgattcgtt tacctccgtt
     9421 tactttttca gaggcaacga gagcattgtc tcccggagag caatagagag tgatccgctt
     9481 gactgcaggt cgaagaatgg gtgctgcttt cttaaaatct tcgagagcaa agtcaggcgc
     9541 gttgaatata acttccccga gtcttggtgg cggccctcct tctgtgaggg cgggaatcac
     9601 aacctggtgg cccatgctgt gaacaatcag atgtacggtg gatccgcctc cgctgagtgc
     9661 ggtgatgaat tcgcggaatt gatttctgga cttgcgtgcg ttctgaaaat tcatctcata
     9721 ggtaccggat acattgaggc tggatagaat tccatcatcg cttccggcgg gccaggtgaa
     9781 gagcacgaca gggcctttga atcgaagatc gtatttgatt tgtgctgcac ggtaaaccgc
     9841 ctcttcgaat ttcacattga agccgtgaac aaaaacgaga acttgttttg cagactgcga
     9901 ggccgccact attcc
//
LOCUS       DAORFF010000055         9874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3171_length_9874_cov_3.745697, whole genome shotgun sequence.
ACCESSION   DAORFF010000055 DAORFF010000000
VERSION     DAORFF010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9874
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3171_length_9874_cov_3.745697"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PKK76_03680"
     CDS             <1..669
                     /locus_tag="PKK76_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67594.1"
                     /translation="RTGALYAELGFVQGAYSGQGIRHQTQPALTEISQLTFLRASGER
                     QSLLLLESAAAPDVRARGSGPRFAFEYFLFPWLEIGRSFEPTVVRVHDVVAAQKLIWQ
                     YRAIAPAVGTFYSSGNLNGADFLGFQRSTVKSQAYLGGGYEAAVYLPFWVVSPFARLS
                     GDPFSGYASVAAGVKVFIEKFYISAEGVASRQRVPLRNFNGSFTYRRAEETGFRICAG
                     YRIH"
     gene            666..1421
                     /locus_tag="PKK76_03685"
     CDS             666..1421
                     /locus_tag="PKK76_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67595.1"
                     /translation="MNLFELSEEDFLRRLEDDMAITGDLGELFPRMVAYNVPYFVLSQ
                     YWPEERTNLLKDPLFPAFCVAEAGWKGRLENLKGSLDLSSGRLTCEKTYVMQCDVMYV
                     LVWAGEDLALCRVPRNASQTWTSRQEDTVRFVSKTGSVRDHFIVSVEQTLKREEYVTL
                     RKRNYVRAGMTIPRREMTGLAILAFGYLRFLKEDVSDLVGLRSELLQARATGELGAGA
                     VHAASQIFHQLLRVLPGDAHPFWGRAGSLFFQR"
     gene            complement(1430..4201)
                     /locus_tag="PKK76_03690"
     CDS             complement(1430..4201)
                     /locus_tag="PKK76_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNL67596.1"
                     /translation="MPELSGQVKVFRAHFTAKKLVLQTQSISIARMQELLPLSAEVRD
                     EPVTASQRLSEFQFAPAGTDPGLALRFLDAFRVRLSHSFDHLSSLSNSRTRLLAHQIE
                     AAHTVVNALRPRFLIADEVGLGKTIEAGLIIKELMFRRGYHNILICVPAPLVVQWQSE
                     LRNRFNEEYTILQRSNYREVMEGKTRHMRIITSIDFIKNPRYAESVLRKRWDLAVFDE
                     AHRLRRDYNKVTVAYAFAEQVAEKCEALMLLSATPFRGKLEELFYLIRLIDPHVLGPL
                     SSFLQEEASGRTADLKRKLSQVLIRRRKVEVGGFTKRHAQTIRFELSPEERAFYDETT
                     EYVRREYNLALAEENRAVGFVMLVFQKLLDSSTRALMRALTNRKMMLERLVASSQTLP
                     ESPDESEWEDQEAPEELVGRVRDRRTWAELRKEILTINRLLLLGRAIETDRKLIKTKE
                     TLQLLKSRGTNKFVIFTQFKSTVDYLCENLTEWKVIPFHGSLRMAEKEQAIADFKDFA
                     DILICTEAGGEGRNLQFSNVLINYDLPWSPLKIEQRIGRVHRFGQTKDVYIFNFATRD
                     TVSERVLEVLTNKIKLFEESIGASDALLGAVQDEVEFQRDLMEFITNKKTRKELNEEL
                     AAKLKTAESGYSKLHTLVSPSRMNFNWDDYYGYTKRERSINSRTLEKITSNYLADVPQ
                     AGSLEKSGADYLLQRNGVRKQASFDSERALERDSLEFLALGHPLVEEAVDHYLSLPVG
                     KSIHAIRDSRNGYYFIYSLRYSSNTMELTSVFCSQDKPEEPLVLPDLILAPGHDAVAA
                     SFNADATENILLRRAAAAAAKAVEQDAETRRLRMEETLKTVFRKEELKVESSHNKHLK
                     GLREKQDIQKMRATMHPVFETRASLAKTEKEISRTQSARQIDLERIRRMSKIKAEIEL
                     LQIYRLMDR"
     gene            4211..5002
                     /locus_tag="PKK76_03695"
     CDS             4211..5002
                     /locus_tag="PKK76_03695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNL67597.1"
                     /translation="MRSSDFKTILITGASSGIGLELARVCAEKGHDLVLIGRHAGRLK
                     DAATELAAHGVKVHTIRKDLSEPEAADAVLKELTRKKISVDALVNNAGFGDHGAFEKS
                     DWEKVSMMIMTNVHALTHLTRLVLPSMRAHGRGIIVNIGSTAGLQPGPYMAVYYATKA
                     FVNHFSEALDYELRGSGIRVTCILPGATSSGFQEAASLNGSLIDRLAKLATSRQVAEF
                     TYAKMMAGRRFAIHGVFNRILAFSLRLTPRFLVTFMAAKINGEGS"
     gene            5174..5788
                     /locus_tag="PKK76_03700"
     CDS             5174..5788
                     /locus_tag="PKK76_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67598.1"
                     /translation="MMNKTKHSFLLILGALFAFSIQCKSAANLRATDTLDKADGVLIF
                     SMRYFKVQNATKIEEPLGSFYREGMRARSGNSTYFVYDGTTLFLSDGSERRANISHPF
                     LLKFAAGDIVIQSIRSTGRYPVSGGTMNLTKLAPVGVRMKVRPQAITYGGEITITEDY
                     ATKPTTFRCEIASEMSTDQIKDALSGFQNTSGFEIKEELGTCPN"
     gene            complement(5844..6164)
                     /locus_tag="PKK76_03705"
     CDS             complement(5844..6164)
                     /locus_tag="PKK76_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67599.1"
                     /translation="MIKKTIGIIALFAAAYGVFWITSKRHELRNFPGIISSFYSKEMC
                     SCLFVAGQTEEFCNNYARQWVPIQGSPVVDRERKTVRVTGLFRTNEAVYTGEKTGCVL
                     RSGD"
     gene            complement(6174..6617)
                     /locus_tag="PKK76_03710"
     CDS             complement(6174..6617)
                     /locus_tag="PKK76_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67600.1"
                     /translation="MIRRHSLHMYAPIAFALLWTSCAIDGSDVVSGGEMIDRIRTAVS
                     IKVGICYPGDASNVLLAKEVTTRFDRGQFYSTKDVDRCIQDFDALPCAFFYQNFGRQS
                     VSLFVGNVYAFVCPNLSDKEFFSPTYMQGNFPESSSTKHSSTSSN"
     gene            complement(6614..7807)
                     /locus_tag="PKK76_03715"
     CDS             complement(6614..7807)
                     /locus_tag="PKK76_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNL67601.1"
                     /translation="MRIATLALFFALVNCTRIQNPSEPLPASPPAPQKFDIPSRSYWP
                     TAGWRTSKPEEEGLKPEAIAKLNQYLFTRTGDDLDRNGVRTDGALIIRGGKIVYERYA
                     RGYDENRPHLIWSSAKSFISALIGIAVKEGKLSLSDSASKYFAEMKDGQKSEIKVEHI
                     LQMSSGLAWNEGYEASPLDSSVIAMLYTAGRTDMARYTASYPLRYRPGTRWYYSSGDT
                     NMLSAILRKTMSEEEYQNYPWAKLFEPLGMKNMTFERDGSGTFVGSSYIYGSPRELAK
                     FGFLYLNDGVWDGKRILPEGWVTLSRTMAPAFYAHPEREDAWDGNYGAQWWQNGDAPG
                     LKAPWPDAPMDTYVASGHWGQRIYVIPSLDIVAVRVGDDRRHPPEGGFSDNEFLKWIA
                     QAAKK"
     gene            complement(7857..8813)
                     /locus_tag="PKK76_03720"
     CDS             complement(7857..8813)
                     /locus_tag="PKK76_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL67602.1"
                     /translation="MTALKIKSAWFYISFAFALTACSGQIKNGERITKDPLILKDQEA
                     TSSQPLPWKMVRTPEAAALNDSGVEAVHDLRLDDAEKLFEDSIQKSPLYGLAYLNLAR
                     LYWVAEEENNSSKVFARLAVSGISYEDLLKTADRLYEYSRTRESVALLEQLSQIPQAG
                     HLPSLRLGNYYLGQALYAQADAHFDRVLAKKPGNADALFARGFIRFQANDFERAASFL
                     EAAKTNGSQEPQLCRLYLQSLFRTNKMEKAKTEQANCRGTDPDLAEIKIRILLVLNPF
                     EDVSRIVAELKQEDAAALEMRVFGSNNRSALRQIQKELELGY"
     gene            complement(8879..9778)
                     /locus_tag="PKK76_03725"
     CDS             complement(8879..9778)
                     /locus_tag="PKK76_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67603.1"
                     /translation="MKQKLSLITFAFTLISCSTIQTTENEDLQKQEREKLEQQTREDQ
                     TRADQKKRFAEEEKKRLELLETAKKDREKLDAQEKEKARMAEMQRREEARKAEEKRAA
                     DEQKRTDEKKTAEMQIADQKKEEIKKQEEAARAAAEERERQRILEEQRLAEERRKAFE
                     ARQNRLDQIDPDRKAFFRIEMQNKVGSLSCRRDSVIIYRNKRPLQFQGYTMAALPDAD
                     IDSPFSFGGKDLADALRGKKGHFVALEDRSTTRPLLCDGRNGSFRIIDMDSDGEGLWL
                     GLEMENSNGEFYKIYTESVPLGR"
BASE COUNT         2175 a         2430 c         2518 g         2751 t
ORIGIN      
        1 aggaccggtg cattgtatgc ggaactgggg ttcgttcagg gagcctactc aggtcaggga
       61 attcgccacc agacccagcc cgccttgacc gagatttcgc agctgacctt tttacgagct
      121 tctggggaac gtcagtcgct cttgctcctg gaaagcgcgg ctgcgccgga tgtacgcgca
      181 cgcgggtcgg ggcctcgctt tgcttttgaa tattttctct ttccatggct ggaaatcggg
      241 cggagttttg agcccaccgt tgtccgggtt cacgatgtag tcgccgcgca gaaattgatc
      301 tggcagtacc gggccatagc acctgcggtc ggtacgttct atagttcggg caatctcaat
      361 ggcgctgatt tcctgggatt tcagaggtcc acagtcaagt cccaggccta cctgggcggc
      421 ggttatgagg ccgccgtcta tctgcctttc tgggtggtat cgccttttgc ccgactgtcc
      481 ggtgatccgt tttctggata tgcgagtgtg gcggcaggtg tgaaggtctt tattgagaaa
      541 ttctatattt ccgcagaggg agttgcctct cgtcagagag ttccactccg gaatttcaat
      601 ggatcattca cgtatcgaag ggcagaagaa acaggcttta gaatttgcgc ggggtataga
      661 attcattgaa tctatttgaa ctaagtgaag aggactttct gcgtcgtctc gaagacgaca
      721 tggcgatcac gggggatctg ggtgagctct ttcctcgtat ggttgcgtat aacgttccct
      781 attttgttct atcccagtat tggcccgaag aaaggacaaa cttactcaaa gatcctctgt
      841 ttcccgcctt ctgcgtcgca gaggcgggct ggaaaggcag gcttgaaaat ctgaaaggat
      901 cgctcgatct gtcgtctggt cgcctaacgt gtgaaaagac ctatgtgatg cagtgcgatg
      961 tgatgtacgt tttagtgtgg gccggcgaag atctggctct ttgcagagtt ccgcgcaatg
     1021 cttcgcagac gtggaccagt cggcaggaag acactgtgcg atttgtttcg aaaacaggta
     1081 gcgtgcgcga tcatttcatc gtgagtgtgg agcagactct gaagcgagag gaatatgtaa
     1141 cgctgcgaaa gcgaaattat gtgcgagccg gtatgaccat tcctcgtcgg gaaatgacgg
     1201 gactggccat cctcgcgttt ggatatttgc gatttttgaa agaggatgta tctgacctgg
     1261 ttgggcttcg ctctgagctt ttgcaggcac gcgccacagg cgagcttggt gctggggccg
     1321 tgcacgctgc cagccagata ttccatcagt tgttgcgggt attacccggg gacgctcatc
     1381 cattctgggg tcgcgccggt tctcttttct ttcagagata gaactttctt caacgatcca
     1441 tgagtctata gatttgcaga agttcaattt ctgcttttat tttgctcatt cgacgaattc
     1501 gttccagatc gatttgcctc gcagattgtg tgcgcgagat ttctttttct gttttggcca
     1561 ggcttgctct tgtttcaaaa acaggatgca tggtggcgcg catcttctgg atgtcctgct
     1621 tttctctgag gcctttgaga tgcttgttgt gacttgactc caccttgagt tcttctttgc
     1681 ggaagactgt tttcaatgtt tcttccatgc gcagtcttct cgtttctgca tcctgttcga
     1741 ctgcttttgc tgcggctgca gcagctcttc tgagtagaat gttttccgtt gcgtccgcgt
     1801 tgaatgaagc cgccactgcg tcgtggcccg gagcgaggat caaatccggc aaaacgagcg
     1861 gctcttctgg cttatcctgt gaacaaaaca cactcgtcag ttccattgta ttggaggagt
     1921 aacgcaacga gtagatgaaa tagtaaccat tgcgtgaatc tcgaatggcg tggatgctct
     1981 taccaacagg gagagataga taatgatcca ctgcctcctc tactagagga tggcccagtg
     2041 ccaggaattc gagcgagtct ctttccagcg ctcgttccga atcaaaggaa gcctgtttgc
     2101 gcactccgtt cctttgcaga agatagtctg cgcccgattt ctcgagagac cccgcctgag
     2161 gtacgtctgc gagatagttg gaagtgatct tttccagagt tcttgagttg atgcttcttt
     2221 ctcttttcgt gtaaccgtaa taatcgtccc agttgaagtt cattctggaa ggagagacga
     2281 gagtgtgaag cttggaatat ccgctctctg cagtctttag tttggctgcg agttcttcat
     2341 tgagttcttt gcgcgttttc ttgtttgtga tgaattccat cagatctctc tggaactcca
     2401 cttcgtcctg cactgctccg aggagcgcat cagaggcgcc aatgctttct tcaaacagtt
     2461 tgattttgtt tgtcagaact tcgagaactc tctcagaaac tgtatcgcga gttgcaaaat
     2521 tgaaaatgta aacgtctttt gtttgaccaa atctgtgaac gcgaccaatc ctctgttcga
     2581 ttttgagagg agaccagggc agatcgtagt tgatgagcac atttgagaat tgcaggttac
     2641 gaccttctcc gcctgcttcg gtgcaaatca aaatgtcagc gaaatctttg aaatccgcga
     2701 ttgcctgttc cttctccgcc attctaaggg agccgtgaaa cgggatcacc ttccactctg
     2761 tgaggttttc gcatagatag tcgacagtgg acttaaactg tgtgaagatt acgaatttgt
     2821 ttgtgccccg cgatttgaga agttgaagtg tttccttggt tttgatgagt ttgcggtcag
     2881 tttctatcgc gcgacccagc aaaaggagac gattgatcgt gaggatttct ttgcgaagct
     2941 cggcccatgt ccgtctgtct cgcactcgcc caaccagttc ttccggcgct tcctgatctt
     3001 cccattcgga ttcatccggg gattcaggca gggtttgcga agaagcaacg agcctttcga
     3061 gcatcatctt tcgattggtc aatgcgcgca tgagtgcgcg agtggatgaa tccagaagtt
     3121 tctggaatac aagcataacg aagccgactg cacggttctc ttctgcgaga gccaggttgt
     3181 attctcgcct gacatattcc gtagtttcgt catagaatgc tctctcttct ggggaaagct
     3241 cgaagcgaat cgtctgcgcg tgtcgcttcg tgaatcctcc cacctccact ttcctgcgtc
     3301 tgatcaatac ttgagaaagt tttcttttta gatctgcggt tctgccggat gcttcttcct
     3361 ggagaaagga cgaaagcggc ccgagtacgt gcggatcgat gagccgaatc aggtagaaca
     3421 gttcttccag tttgccgcga aatggagtgg ctgaaagtag catcaatgct tcgcattttt
     3481 ctgcaacctg ttctgcaaag gcataagcca ccgtaacttt gttgtagtcc cggcgcagtc
     3541 tgtgggcttc atcgaacact gccagatccc atctttttct gagaacggat tccgcataac
     3601 gtggattttt tatgaaatca atcgacgtga tgattcgcat gtgacgggtt ttcccttcca
     3661 tcacttcacg atagttggag cgttgaagga ttgtgtattc ttcattgaaa cggttcctga
     3721 gctcagactg ccattgcact acaaggggtg ccggaacaca gatgagtatg ttgtgatatc
     3781 cgcggcggaa cattaactcc ttgatgatga gcccagcttc gatggtcttt cccagtccta
     3841 cttcatctgc gatgagaaag cgcgggcgca gagcattgac cacggtatgg gccgcttcta
     3901 tttgatgggc cagcaaacgt gtgcgggagt tggagagact ggagagatga tcgaatgaat
     3961 gactgagacg aacacgaaat gcgtccaaaa atcgcagggc caatccgggg tccgtgcctg
     4021 caggtgcaaa ctggaactcg gaaaggcgct gggatgctgt gaccggttcg tcccgtacct
     4081 ccgcagagag cggcaaaagt tcctgcattc gagcaatgct gatcgattgc gtctggagta
     4141 caagtttttt tgccgtgaag tgtgcgcgaa acactttgac ctgtcccgat agttccggca
     4201 caacgcctcc atgcgatcat cagactttaa aactatcctg atcaccgggg cttctagcgg
     4261 aattggcctc gaactcgcaa gagtttgtgc agagaaaggc cacgatctgg ttctcattgg
     4321 gaggcacgca ggcagactca aagatgcggc cacagagctg gccgcgcacg gagtaaaagt
     4381 ccatacaatt aggaaggatc tgtcggagcc ggaggctgcg gacgcagtct tgaaagaact
     4441 gactcgcaaa aagatctcag tggatgcgct cgtaaacaat gcaggattcg gagaccacgg
     4501 cgcatttgag aaatcagatt gggaaaaagt gtccatgatg atcatgacca acgtgcacgc
     4561 tctcactcat ctgactcgcc tggtgcttcc atccatgcgc gctcacggtc gcggaatcat
     4621 tgtgaatatt ggatcaacag ccggactcca gcccggtcca tacatggccg tttactacgc
     4681 gaccaaagca ttcgtgaatc atttttctga agcactggac tatgaactgc gcggttccgg
     4741 gattcgcgtg acttgcatct tgcccggagc cacgagctcc ggatttcagg aagcagcatc
     4801 actcaacgga agcttgattg atcgacttgc gaaactcgcg acgtcacgtc aggttgctga
     4861 attcacgtac gcgaaaatga tggcgggaag acgctttgcg attcacggag tgttcaatcg
     4921 catccttgcg ttttctctga ggctcactcc gcgttttctg gttacattca tggccgcgaa
     4981 gatcaacgga gaaggtagct gaacaggctt cgtgtcagca aaaaagagtc ttgaagggct
     5041 tcggagaccg gcatcacact cgaccggagg tccagatatt ttcggaatct ggatttcaga
     5101 ttgcccgaaa tcatgatgct gttgactttg agttaaaacc aaacttaata ctaaggtttt
     5161 ttgaggtttc agcatgatga acaaaacaaa gcattccttc cttctgattc tgggcgcgtt
     5221 attcgctttc tctattcaat gcaaatcggc tgcaaacctg cgtgcgacgg atacgcttga
     5281 caaagcggac ggagtgctga ttttttcgat gcgctatttc aaggtgcaaa acgcaacgaa
     5341 aatagaagaa cctctcgggt cattttatcg tgagggtatg agagcacgtt ccgggaactc
     5401 cacatatttt gtatatgacg gaaccactct tttcctgagc gacggttccg aacgccgcgc
     5461 caacatcagt caccctttct tgctcaagtt tgcagcgggt gatattgtca ttcagagcat
     5521 ccggtctact ggccgttatc cggtttccgg cgggactatg aatttgacaa agctcgcccc
     5581 ggttggagtt cgaatgaaag tgcgccccca ggcaattact tatggtggag agatcacaat
     5641 caccgaagac tacgctacga aaccaaccac atttcgatgc gagattgcca gtgaaatgag
     5701 cacagatcag atcaaggatg cattgtcagg attccagaac acgtctgggt ttgagattaa
     5761 ggaagagcta ggtacgtgcc caaactgaaa agataactac tcagtgttca tggccgcgaa
     5821 gatcaacgga gaggtcgttc ggatcagtca ccggaccgaa gaacgcagcc tgttttttct
     5881 ccagtgtaga cggcctcatt ggttcgaaac agccctgtaa cgcgcactgt ctttctttct
     5941 ctgtccacaa caggcgatcc ctggatgggc acccactggc gcgcatagtt gttgcagaat
     6001 tcctctgtct gtcctgccac gaataaacag gagcacattt ctttgctgta gaaagaagaa
     6061 atgattcccg gaaagttgcg gagttcatgt cgcttgcttg tgatccagaa tacgccgtaa
     6121 gcggcggcaa acagtgcgat gattcctata gttttcttaa tcattatatt tcctcaatta
     6181 cttgaagtgc tgctgtgttt ggtgctagat gattccggga aatttccctg catatacgtt
     6241 gggctaaaga actccttgtc gctcaagttt ggacagacaa atgcatacac gttccctaca
     6301 aagagggaaa cggattgcct tccaaagttc tgataaaaga aagcgcacgg aagggcatca
     6361 aaatcctgaa tgcacctgtc cacgtctttg gtggagtaaa actggccgcg atcaaagcga
     6421 gtcgtgactt cttttgcaag gagcacattg cttgcatctc ccggatagca gatcccaact
     6481 ttgatgctca ctgctgtgcg aatgcgatca atcatctctc cgccggaaac aacatcagat
     6541 ccatcaatcg cgcaggaagt ccagaggagt gcgaaagcaa tcggcgcgta catgtgcaga
     6601 gaatgtctgc ggatcatttc tttgcggcct gcgcaatcca tttaagaaac tcattgtctg
     6661 agaatccgcc ctctggtgga tgtcggcgat catctccaac gcgcactgcg actatatcca
     6721 gacttggaat cacgtagatt ctctgccccc agtgtccgct cgccacatag gtgtccatag
     6781 gtgcgtctgg ccatggagcc ttcaggccag gtgcatctcc attttgccac cactgtgctc
     6841 cgtagttgcc atcccaggca tcttctcttt ctggatgtgc gtagaaagcc ggggccatcg
     6901 tacgcgaaag agtgacccat ccttctggca aaattctttt tccgtcccag acaccgtcgt
     6961 tcaaatagag gaatccaaat ttcgcgagct cccgcgggga cccatagata taagatgagc
     7021 cgacgaaagt tccagacccg tctctttcaa aagtcatgtt cttcattccc aggggctcaa
     7081 agagttttgc ccatggatag ttctggtatt cttcttcgct cattgtcttg cgaagaatgg
     7141 cgctgagcat attcgtatct ccgctcgaat agtaccatct ggtaccggga cgatatctca
     7201 gaggatagct tgccgtataa cgggccatgt ctgtgcgtcc ggctgtgtag agcatggcga
     7261 tcacggatga atcgagagga gatgcttcat atccttcgtt ccatgcaagc ccggagctca
     7321 tctggagaat gtgttctact ttgatttctg acttttgtcc gtctttcatc tccgcgaaat
     7381 atttggatgc agaatcagaa agcgagagct tcccttcttt cactgcaatt ccaatcagtg
     7441 cgctaatgaa acttttcgca gaagaccaga tcagatgcgg acgattttca tcatatccac
     7501 gtgcgtaacg ttcgtagacg atttttcctc cgcgaataat caaggctcca tctgtgcgca
     7561 ctccatttct atccagatcg tcgccggtgc gtgtgaacaa atactgattg agcttcgcga
     7621 ttgcctccgg cttcagtccc tcctcttccg gtttagaggt tctccatccg gcggttggcc
     7681 agtaggaacg agatggaatg tcgaatttct gcggtgcagg tggcgatgca ggaagtggct
     7741 cagaagggtt ttgaattctg gtgcagttta caagtgcgaa aaacagggcc agagtggcga
     7801 tgcgcatagt ccgattgcaa cgataggaaa ggacgctaca agcaaaaaaa ccccaatcaa
     7861 taacccagct ctagttcttt ctgaatttgc cgcagagcgg atcgattgtt ggatccaaag
     7921 acgcgcattt caagggccgc ggcgtcttcc tgtttcaatt ccgcgacgat ccgggaaaca
     7981 tcctcgaacg gatttagcac aagcaatatg cgaatcttga tttccgccag atctggatca
     8041 gttccgcggc agttcgcctg ctctgttttt gccttctcca ttttgtttgt gcgaaacagg
     8101 gactgcagat aaaggcgaca gagttgaggc tcctgcgatc catttgtctt tgccgcctca
     8161 aggaagcttg ccgctctttc aaaatcattt gcctgaaaac gaatgaagcc gcgtgcgaaa
     8221 agcgcgtccg cgtttcccgg tttttttgcg agaacgcgat cgaagtgggc atctgcctgc
     8281 gcatacaagg cctgacccag ataataattc ccgagccgca gagatgggag gtgtcctgcc
     8341 tgcggaatct gactgagttg ttccagcaat gctacggatt ctctggttcg ggaatactcg
     8401 tagaggcggt ccgccgtttt gagcaggtct tcgtaagaga ttccggacac agccaggcga
     8461 gcaaagactt ttgacgaatt gttttcttct tctgccaccc agtagagtcg tgctagattt
     8521 agatacgcaa ggccgtataa cggcgatttt tgaatggaat cttcaaacaa cttttctgcg
     8581 tcatccagac gcaaatcatg gactgcctcc acaccactat cattgagcgc ggctgcctcc
     8641 ggagttcgca ccattttcca cggcagcggc tgactgctgg tggcttcttg atctttgaga
     8701 atgagcggat cctttgtgat gcgttcgccg ttcttgatct ggccgctgca agcggtcaac
     8761 gcgaaggcaa agctaatgta gaaccaggcg ctttttattt tcagtgcggt cacgggttca
     8821 tgcttggttt gtttgcagat gggtcaattc gcttccaggg actcttgcgc atttatcttc
     8881 atcttccgag cggaacggac tcggtgtata ttttgtagaa ttctccattt gaattctcca
     8941 tctcaagccc aagccacagt ccctctccat cagaatccat gtcgatgatg cggaacgacc
     9001 cattccttcc atcgcataag aggggccttg tcgtggatct gtcctcgagt gctacgaagt
     9061 gtcctttttt gccgcgcaag gcgtccgcca gatcttttcc gccaaatgaa aagggagaat
     9121 ctatatctgc gtcgggtaga gctgccatgg tatagccctg aaattgtaac ggtcttttgt
     9181 ttctgtagat gattacacta tcgcggcggc aggaaagact tcctactttg ttttgcattt
     9241 caatgcgaaa gaatgctttt ctgtccggat caatttgatc gagtcgattc tgtcttgctt
     9301 caaaggcttt tcttctttct tctgccagcc tttgttcttc cagaatcctt tgtctttcgc
     9361 gctcttctgc ggcggcccgt gcagcctctt cttgtttttt gatttcttcc ttcttttgat
     9421 ctgctatttg catttctgcg gtttttttct catccgttct cttttgttcg tcggcagctc
     9481 ttttttcttc tgctttccgt gcttcttcgc gtctttgcat ttctgccatc ctggctttct
     9541 ctttctcctg tgcgtccagt ttctctctgt ctttctttgc agtttccagt agttcgagcc
     9601 gtttcttttc ttcctccgcg aacctcttct tctgatccgc gcgtgtttga tcctctcgcg
     9661 tttgttgttc gagtttttct ctttcctgtt tttggaggtc ttcgttctcc gttgtctgga
     9721 tcgtgctgca agaaatcagc gtgaacgcga aagtgatcag cgagagcttc tgcttcaatg
     9781 ttctactccc gcgatcaggc gcagggcgcg ctgcgcaaga taggcgtctt tcttttttct
     9841 aacggtggat ttgagtgact cctttttccc ctgc
//
LOCUS       DAORFF010000056         9857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3177_length_9857_cov_3.452357, whole genome shotgun sequence.
ACCESSION   DAORFF010000056 DAORFF010000000
VERSION     DAORFF010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3177_length_9857_cov_3.452357"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PKK76_03730"
     CDS             complement(<1..318)
                     /locus_tag="PKK76_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67604.1"
                     /translation="MPSVSTSIPRRDRSSFQANVLSRWLRFTFALIPGLLTSCLTWSV
                     TETLKEKKPEYLKFDEPPAYYAGIPYADEKRSGLIGLFEVRGTTGFYKYKVNIEFSRR
                     DEPV"
     gene            426..584
                     /gene="rpmG"
                     /locus_tag="PKK76_03735"
     CDS             426..584
                     /gene="rpmG"
                     /locus_tag="PKK76_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNL67605.1"
                     /translation="MREIVKLESTAGTGFFYTTTRNKKANQEKLEMKKFDPKAKKHVK
                     FVEKKLSK"
     gene            594..1496
                     /gene="ispE"
                     /locus_tag="PKK76_03740"
     CDS             594..1496
                     /gene="ispE"
                     /locus_tag="PKK76_03740"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNL67606.1"
                     /translation="MNLASVSLESPAKINVGLRILGRRMDGFHELQGIFVPISFGDQV
                     QIKESEAPGFHFTLHTQNCLTGEAARRFDESTERGDIKQNTVYRALHALAPFIQDANL
                     HLEITLVKRVPAGAGLGGGSSNAGVVLKHLKQRLSLRDERAHAAAVRAGADVPFFLLD
                     GSAFVEGVGDRLRPIDFPHGQGVLCLSDFYISTPEAYRDLKRPLQGDAVQESRTSFER
                     WQEAAFSLWKDDGFLHNDFEEVAFQKHPDLARVKQALKDRGAFASLTGSGSAIYGLLP
                     EGAADLAKEMAERFPGCRFESFQF"
     gene            1502..1573
                     /locus_tag="PKK76_03745"
     tRNA            1502..1573
                     /locus_tag="PKK76_03745"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1534..1536,aa:Gln,seq:ttg)
     gene            1590..2342
                     /locus_tag="PKK76_03750"
     CDS             1590..2342
                     /locus_tag="PKK76_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNL67607.1"
                     /translation="MNQRAAVVLAAGKGTRMKSELPKVAFPVLGRAMILHVLDHLSEV
                     GFDRIVLIVGYGREIVEGLVKDRKENIQFALQAEQKGTAHAFLCAEEALKDFHGSVVV
                     TCGDMPMIRTSTFKRLIEQHAAEKNASTVLSAVLENPKGYGRLVRGADGKLARIVEEK
                     DATDEIRLIRETNSGTYVFESPEIFSVLRSIDTNNKQNEYYLPDAIKILNERKQSVGV
                     CFTQDAEEALGANSQEDVAVLESIMNRRLAGV"
     gene            2339..3277
                     /locus_tag="PKK76_03755"
     CDS             2339..3277
                     /locus_tag="PKK76_03755"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNL67608.1"
                     /translation="MSFHIVAFSGNANLPLAQEICNHLGIPLGRANVKQFSDGEISIK
                     LEENVRGRDVFVIQPISAPANDHLMEVLLMTDALRRASADRITLVIPYYGYGRQDRKV
                     EPRVPISARAVADLLECMLPTRLLTMDLHADQIQGFFRLPVDHLYAAPVFVEYLRNKN
                     LDQPVVVSPDSGGVERARFLARQINAGLAIIDKRRPRANVAEIMNVIGEVSGKVCILY
                     DDMIDTAGTITKAAVALKEHGAKSVLCCATHGVLSGPAIERLRDSILDEVIISNSIRL
                     DPSKKIDKITVLSVARLVGEAIRRIHNEESVSSLFL"
     gene            3308..3955
                     /locus_tag="PKK76_03760"
     CDS             3308..3955
                     /locus_tag="PKK76_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNL67609.1"
                     /translation="MEVKLSAKRRQETGKNEMNRLRKAGDVPGNIIYEGNSVMISFAK
                     ASIQKLVQSGLRTSTVIELDVDNQPNRVIVKEVQRHPVNGEILHVDFYRLTPGRRLTV
                     NIPVTTKGNAIGVKDGGALEQFYNSVKIRTTPEALKETIEVDVTNLGVGDGVRLSALP
                     VSKEWDIRLEGDPMILKVSRSRMTLETDAAPGAEGAPAAAAAAAPAAGAKAPEKK"
     gene            3995..4558
                     /gene="pth"
                     /locus_tag="PKK76_03765"
     CDS             3995..4558
                     /gene="pth"
                     /locus_tag="PKK76_03765"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNL67610.1"
                     /translation="MKLIIGLGNPGEKFQNNRSNIGFKILDVLANNNNIEIRTKKKKS
                     LIGRGEFDGEDIVLLKPQTFTDLAGEAALYIASFLKIKPPDIVVIMDDFGLPLGKLAV
                     EKGGNDYSHPAIKNLAIALKSPEFIRLRVGILGKTAGKLTRDKYVSSEFEPMEILQLI
                     SIINDAEAAVRSISSGDINEVIEKFKQ"
     gene            4602..6596
                     /gene="ftsH"
                     /locus_tag="PKK76_03770"
     CDS             4602..6596
                     /gene="ftsH"
                     /locus_tag="PKK76_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004496851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNL67611.1"
                     /translation="MNRNARYFILILFGIVVIMMLMGGPSKLLQPLEKPETITFSQFK
                     QMLTPEGRAATGSIFTRASDKEEDSIVLRIAPRKITGRYLKPGVSIEKSDSLDKVRSK
                     TYPFEAEISQGAITEKIQQSLDESGVIYKFENPEEGSLFSSILQILPFVLLIGFLWMF
                     MMRQIQATGNRAMSFGKSKARLMPEGKKKLNFHDVAGCDEAKTELVEVVEFLKDPKKF
                     QAIGAKIPKGVLLVGPPGTGKTLLARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRD
                     LFEQAKRQAPCIVFIDEIDAVGRLRGAGLGGGHDEREQTLNQMLVEMDGFEENEGIIV
                     VAATNRPDVLDPALLRPGRFDRQIVVDIPDVKGREAILKIHAKKVPLTSDVSLQNIAR
                     GTPGFTGADLANLINEAALLTARRNKRRVTQDELEEAKDKVMMGPERRSFMITPKEKE
                     VIAYHEGGHALLGTLLAYAEPVHKVTIIPRGRALGLTQQLPTDDKHIHPSRYWEDRIC
                     VLMGGLLAEDIVFGDRSTGASNDIQVATNIARRMVCEWGMSKKIGTINYSTHNDNVFL
                     GREIQSPRTYSDETASTIDEEVNRIVTEQREKGRSLLNQHREKLERIAKALLAHESIS
                     GTELNEIVGADASESQKRSLDPRHPHGMEPASEPSVAPAS"
     gene            6639..8195
                     /locus_tag="PKK76_03775"
     CDS             6639..8195
                     /locus_tag="PKK76_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67612.1"
                     /translation="MDDDLVKLLANPFEEDRLQARAMLAGRAQDLGEGETHDFLRDLR
                     AFRGSPDTVLYTHRSSAETVQQRLFSETLAPGARHAIEPYRARLTQHITSLLKNFKVF
                     VETCGEEILLREIKRLERRYFDTDPTPQQINFLKLKRIYEKIKTFQELARVDWREVFK
                     VVDTLTVIEETRNMARILEKEVLRSIPLLMRRTDHFLESVSSYMGGGEEARDPVTGSY
                     RLAVKYNAELAYTIGHMLGDEIFQEAQEEEKKEETDETEEAETFSGSIARNIVIDTRE
                     KILNKSSLISAPLLGSRDWNRTPAYSMEVPVRQYEECFENFKQSFIVHLEPGVLPANA
                     ASNQMAARLRSGSGESVMDDYVKLVFDLIEEVAQDLVGKDFPGLDKPLIFLYHCGPIA
                     MFNMLLLTMKQRQIGEIQYLDKSGNAIREYPQEMLKKLLIDWWRARFLPLGSEEIDSY
                     INYSRCLDHVKKDYRILYNAGVEVRKKEQPMGLYSGLQRWMRENRARVFGIRRMEVFR
                     RFIPGVFLNF"
     gene            8247..9401
                     /locus_tag="PKK76_03780"
     CDS             8247..9401
                     /locus_tag="PKK76_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNL67613.1"
                     /translation="MKQLPRILPFTKEHDDFRAMAADFFDQEVAPHHEQWEKDGIVPR
                     EIWAKAGSLGLICPNFPEEYGGAGADFLYNLIVIEESARVGASGFFISLHADVIAPYV
                     LHHASDEQKQRWLPGIIDGTKILAIAMTEPGAGSDLAGITSTAIDKGDHYLVNGSKTF
                     ISNGYLSDLIITVAKTDPEKEINGISLLMVERGMEGFERGKRLKKIGLNAQDTSELFY
                     HDVKVPKANLLGKKGYGFRYLMSELATERLVLAASSMRGCEVALEETLKYVKTRKAFG
                     KSLGSFQNTQFKLADAATEVAAARCFLDQVILAFMRGDKVVVEASQAKLLCSELLKKT
                     VDTCLQFFGGYGYMMEYPIARAFVDARVQSIYAGTSEIMRGIIASKGLGL"
     gene            9449..>9857
                     /locus_tag="PKK76_03785"
     CDS             9449..>9857
                     /locus_tag="PKK76_03785"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNL67614.1"
                     /translation="MALQADLSGEVPVGALVVELSTLKIVGRGKNEIISRKDPTAHAE
                     LLAIREACAHFQSERMLPSVLVSTLEPCTLCTGAILFARVAEVQYFTPVLSGAGIVRL
                     LDQFGTSYNHRPLLTHIESHQEKARQILVSFFER"
BASE COUNT         2560 a         2395 c         2564 g         2338 t
ORIGIN      
        1 gacaggctca tccctccgcg agaattcaat atttactttg tacttataga aaccggtcgt
       61 acctcgaacc tcaaagagtc caatgaggcc tgaccttttt tcgtccgcat acggaattcc
      121 agcgtaatac gctggcggtt catcgaattt cagatactcc ggtttctttt ccttcagggt
      181 ttccgtgacg ctccaggtca aacagctcgt aaggagtcct ggaatcagtg cgaaagtgaa
      241 tcgcagccag cgcgaaagga cgttcgcctg gaaggaggaa cgatcccgcc gagggatcga
      301 agtagagacc gaaggcatcc gcattcatgg gcgcagttgg agagtgcgtc gagcaatatc
      361 catcgtccgg tggcgagggt gctcctttga cttgactctc tggaacgttc ggacggtctg
      421 cccttatgag agagatcgtt aaactggaat cgactgctgg gacaggattc ttctatacta
      481 ctacgcgcaa caaaaaggca aatcaagaaa agcttgaaat gaaaaagttt gatccgaaag
      541 ccaagaagca cgttaaattc gttgagaaga agctaagcaa ataagccgcc ggcgtgaacc
      601 ttgcgagtgt ctctttagaa agtcctgcta agatcaacgt cggcctgcgg attctggggc
      661 gcaggatgga cggatttcat gaactccagg gcatattcgt gcccatatca ttcggagacc
      721 aggttcaaat caaggaatca gaagccccag gttttcattt cacactccat actcaaaact
      781 gcctcaccgg agaggccgct cgtcgctttg atgaatccac agagcgcggc gacataaagc
      841 aaaacacagt ataccgcgca ctccacgcac tggctccttt catccaggac gcaaacctcc
      901 atctggaaat cactctggtc aaaagagtgc ctgcaggcgc aggtttaggc ggaggaagct
      961 cgaatgctgg agttgtgctc aagcatctca agcaaagatt gtctctgcga gacgagcgcg
     1021 cacatgcagc tgcagtgcgc gcgggcgcgg atgttccatt ttttcttctc gatgggagcg
     1081 cctttgtaga aggagtaggg gatcggctca gaccgattga ttttccacac ggccagggcg
     1141 tgctgtgcct cagcgatttc tatatttcca cacctgaagc atacagggac ctgaaaagac
     1201 ctttacaggg ggacgctgtt caagaatctc ggacatcgtt tgaaaggtgg caggaagcgg
     1261 cattttcctt atggaaagat gatggtttct tgcacaacga cttcgaagag gttgcgtttc
     1321 agaagcatcc agatctcgcc cgcgtcaagc aggcattgaa agatcgtggc gcttttgcat
     1381 ctctcactgg atcaggttcg gccatttatg gtctactgcc agaaggggcc gcagatcttg
     1441 cgaaggagat ggcggagcgg ttccccgggt gccgattcga gtcctttcag ttctaaaaaa
     1501 ttgggccgtc gccaagcggt aaggcagcgg gttttggtcc cgccatccgg aggttcgaat
     1561 cctcccggcc cagcatttca ggggagatcg tgaatcagag ggccgccgta gtacttgctg
     1621 caggaaaagg aacgcggatg aaatccgagc tccccaaagt cgcttttccc gttctgggcc
     1681 gcgcgatgat ccttcatgta ctcgatcatc tttcggaagt cggctttgat cgcattgttc
     1741 tcattgttgg atacggccgt gaaatagtgg aaggcctggt caaagatcgt aaagaaaata
     1801 tacaattcgc acttcaggca gagcagaaag gaacggcaca tgcattcctc tgcgcagaag
     1861 aagcactcaa ggatttccac ggttccgtcg ttgtcacatg cggtgacatg cccatgatcc
     1921 gcacttctac attcaagaga ctcatcgaac agcatgcggc tgagaaaaac gcatctactg
     1981 ttttatccgc agttcttgaa aacccaaaag gatacggaag actcgtgcgc ggtgcagatg
     2041 gaaagcttgc gcgaatcgta gaagagaaag acgcaacaga tgagattcgc ctcatccgtg
     2101 aaaccaattc aggaacgtat gtttttgaat cgcccgagat cttctctgtg ctacgctcta
     2161 ttgatacaaa caataagcag aatgaatact accttccgga cgcaatcaag attctgaatg
     2221 aacgcaaaca gagtgttgga gtttgtttca cccaggatgc ggaagaagca ctgggagcaa
     2281 attcacagga agatgttgct gtactcgaaa gcattatgaa ccggaggctc gcaggcgtat
     2341 gagttttcat atcgttgcat tctccggaaa cgcgaacctt cctctggcac aggaaatctg
     2401 caatcacctg ggcattcctc tcggacgtgc aaacgtgaag caattttcag atggtgaaat
     2461 ctccatcaag ctagaggaga atgtccgcgg tcgcgatgta tttgtaatcc agcctatttc
     2521 cgccccggca aacgaccatt tgatggaagt gttgctcatg acggatgctc ttcgtcgtgc
     2581 gagtgccgat cgcataacgc ttgtcatccc ttattacgga tatgggcgtc aggatcgcaa
     2641 agtagaacca cgcgttccta tttcagctcg cgcagttgca gatcttctgg agtgtatgct
     2701 gcccacccgt cttttgacca tggatcttca cgcggatcag attcagggtt tctttcgtct
     2761 tcctgtagat catctctatg cagcgccagt attcgtggaa tatcttcgca acaagaacct
     2821 cgatcagccc gtcgttgttt ctccggattc aggcggcgtg gagcgcgcga gatttctggc
     2881 acgtcaaatc aacgctggtc tggctatcat tgataaaaga cgcccacgcg caaacgtcgc
     2941 agaaatcatg aatgtgatcg gtgaagtaag cggcaaagtt tgtattctat acgatgacat
     3001 gatcgacact gcaggaacca tcacaaaggc cgccgttgca ctcaaagagc atggtgcaaa
     3061 gagcgttctt tgttgcgcca ctcacggtgt tttatccggc ccggccattg agcggctgcg
     3121 cgatagcatc ctggatgaag tcatcatatc caattccatt cgccttgatc cgtccaagaa
     3181 gatcgacaag atcaccgttc tttccgtcgc aagactggtc ggggaagcca tccgtcgtat
     3241 tcataatgaa gagtccgtca gttcactatt tttgtaacgc catcatctat aaggagaaaa
     3301 caggaaaatg gaagtaaaac tctcagcaaa aagaagacag gaaacaggaa agaacgaaat
     3361 gaatcggctg cgtaaggcgg gcgatgttcc tggaaacatc atctacgaag ggaactccgt
     3421 gatgatttct ttcgcaaagg ctagcattca gaagctggtg caatcaggtc ttcgcacaag
     3481 cacagtgatc gaattggatg tggataacca gccaaaccgg gtcatcgtaa aagaagtcca
     3541 gagacatccg gtcaacggag aaatcctgca cgtggatttc tacagattga ctccgggtcg
     3601 ccgcctgacg gtaaacattc cagttaccac aaaaggcaat gcaatcggcg tcaaagacgg
     3661 cggagcactg gagcaattct acaacagcgt caagattcgc acaacaccag aagctctcaa
     3721 agagacgatc gaagtggatg tgaccaacct tggtgttgga gatggcgttc gcctcagcgc
     3781 gcttcccgtt tcaaaagaat gggacattcg cctggaaggt gatccgatga tcctcaaggt
     3841 atctcgctct cgcatgactc tggaaacaga cgcagctcca ggcgcagaag gtgctccggc
     3901 tgcggccgcc gctgctgctc cggctgcagg tgcgaaagct ccagagaaga agtaagattc
     3961 tgcctgaggt ttttcatatt tcagggaagg cgctatgaag ctcatcattg gtctgggcaa
     4021 tcctggggag aaattccaga acaaccggtc caatatcgga ttcaagattc tcgatgttct
     4081 tgcgaacaac aacaacatcg agatccgcac caagaagaag aaatcgctca ttgggcgcgg
     4141 tgaattcgat ggcgaggata ttgttctgct caaaccgcag acctttacgg atcttgcggg
     4201 agaggcagcg ctgtacattg cttccttttt aaagatcaag ccgcctgata tcgtcgtcat
     4261 catggacgat ttcggtctgc ctctgggcaa gctcgcggta gaaaaaggcg gcaacgacta
     4321 cagccacccg gctatcaaaa acctggccat cgcactgaaa tctcctgaat ttattcgcct
     4381 tcgcgttgga attctgggta aaacggcggg caagttgacg cgcgataagt atgttagttc
     4441 agaatttgaa ccaatggaga tcctccagct catcagcatc atcaatgatg cggaagctgc
     4501 cgttcgatcc atctcgagcg gggacataaa cgaggtcatc gagaagttca agcagtgatt
     4561 gacgtttcct cgtttcgcac ttattatgga gccgtaatac aatgaatcgt aacgcgcgtt
     4621 atttcatcct catcctcttt ggcattgtgg tcatcatgat gctcatggga ggcccgagca
     4681 aactccttca gcctctggaa aagccggaaa ctatcacgtt ttcgcagttc aagcaaatgc
     4741 tgacaccgga gggcagggca gccactggct ctatcttcac acgggcttct gacaaagaag
     4801 aagacagcat tgttcttcgt atcgcgccgc gtaaaatcac aggtcgttat ctaaagccag
     4861 gcgtttcgat tgaaaaatcg gattctctgg acaaagttcg aagcaaaacc tatccatttg
     4921 aagctgagat ttctcagggc gccatcactg aaaagatcca gcaatccctc gatgagagcg
     4981 gagtgatcta taagtttgaa aatccggagg agggtagctt gttcagttcc attctccaga
     5041 tcctgccatt tgttttgctc atcggttttc tctggatgtt catgatgcgt cagatccagg
     5101 caactggaaa cagagccatg tctttcggaa agagcaaggc tcgtctgatg ccggaaggaa
     5161 aaaagaagct gaacttccat gacgttgcgg gttgcgacga agcaaagact gaacttgttg
     5221 aagttgtaga atttctgaaa gacccaaaga agttccaggc aattggagca aagattccca
     5281 agggcgtttt gctcgttggt cctccgggta caggtaagac tcttctcgca cgtgctgttg
     5341 caggtgaggc cggagttcct ttcttctcta tttccggatc tgactttgtg gaaatgtttg
     5401 taggtgtggg cgcatctcgc gttcgcgatc tgtttgagca agccaaacgt caggcgccat
     5461 gcattgtatt catcgacgaa attgatgcag taggaaggct tcgcggagcg ggccttggcg
     5521 gcggacacga tgagcgcgag cagactctca accagatgct tgtggaaatg gacggatttg
     5581 aagagaacga aggaatcatc gtagtcgctg cgaccaaccg tccagacgta ctggatcctg
     5641 ctcttcttcg tccggggcgt tttgatcgcc agattgtagt agatattccg gatgtaaaag
     5701 ggcgcgaagc cattctgaag atccacgcga agaaagttcc tctcacttcg gatgtgtcgc
     5761 tgcaaaacat tgcgcgcgga actccaggtt tcactggcgc ggatcttgcc aacctcatca
     5821 acgaagcggc tctcctcacc gcgcgtcgta acaagcgtcg cgtgactcag gatgaactgg
     5881 aagaagcaaa agacaaagtg atgatgggtc cggagagaag atcgttcatg atcactccga
     5941 aggaaaaaga agtcattgca tatcacgaag gtggtcacgc acttctggga actcttctcg
     6001 cctatgcaga gccggttcac aaagttacca tcatcccgcg cggtagagct cttggtctaa
     6061 ctcagcaact tcctacagat gacaaacaca tccacccgag ccgttactgg gaagatcgca
     6121 tttgcgttct catgggtggt cttcttgcag aagacattgt gttcggagat cgttctactg
     6181 gagcatccaa cgacattcag gtagccacaa acattgcacg tcgtatggtt tgcgaatggg
     6241 gcatgtccaa gaagattgga accatcaact attccacaca caacgacaat gtgttccttg
     6301 ggcgcgagat tcagagccca cgcacttaca gcgatgaaac tgcttctacc attgatgaag
     6361 aagtgaatcg tattgtaaca gagcagcgcg agaagggcag aagtcttctc aaccagcacc
     6421 gtgaaaaact ggaaaggatt gcaaaagcgc ttcttgctca tgaaagcatt tctggcaccg
     6481 agctcaacga aatcgtgggc gcggatgcgt ctgaaagcca gaaacgttct ctggatccca
     6541 gacatccgca tggcatggaa cctgcttccg agccttccgt cgctccagcc tcgtaaaaaa
     6601 ggcttgagac gccctcgggc cggggcgaga atcgaagcat ggacgacgat ttagtcaagc
     6661 tcctggccaa tccattcgag gaagatcgtc tccaggctcg tgccatgctc gcgggccggg
     6721 cccaggacct gggggaaggg gaaacccacg actttctcag agaccttcgc gcttttcgtg
     6781 gcagccccga tacagtcctc tatacccacc gcagttctgc ggaaacagtt caacagagac
     6841 tcttcagcga aactctagca cctggtgcgc gtcatgcgat tgaaccatac cgtgccagac
     6901 tgacccagca catcacaagc cttctgaaga acttcaaagt gtttgtggaa acatgcggcg
     6961 aagaaatctt actgcgcgaa atcaagagac tggagcggcg ctactttgat acagacccga
     7021 ctccgcaaca gatcaacttt ctcaaactga aacggatcta tgaaaagatc aagacgtttc
     7081 aagagctggc ccgtgtggac tggcgcgaag tcttcaaagt agtagatact cttacagtga
     7141 tagaagagac gcgcaacatg gcgcgcattc ttgaaaaaga agtacttcgt tccattccgc
     7201 ttttgatgag acggaccgat catttcctgg aaagtgtttc cagctacatg ggcggaggcg
     7261 aagaagctcg cgaccctgtc acaggaagct atcgtcttgc tgtgaagtac aacgccgagc
     7321 ttgcatatac gattggtcat atgctcggcg acgaaatctt tcaagaagca caagaggaag
     7381 aaaagaaaga agaaaccgat gaaacagagg aggcggaaac ctttagcggc tccattgcgc
     7441 gcaacattgt cattgatacg cgcgaaaaga ttctgaacaa atccagtttg atttctgcgc
     7501 cattgcttgg ctcgcgcgac tggaatcgca ctccagcata tagcatggaa gttcctgtcc
     7561 gtcagtatga agaatgtttc gagaatttta aacaatcctt cattgtgcat ctcgagccag
     7621 gagtacttcc ggcgaatgca gcttccaatc agatggctgc gcgtttgaga tctggatccg
     7681 gcgaaagcgt aatggacgat tacgtgaaac ttgtctttga tttgattgaa gaagtcgcgc
     7741 aggatctggt gggtaaggat tttcccggac tggacaagcc actcatcttt ctctatcatt
     7801 gtgggccgat cgccatgttt aacatgcttc ttctgaccat gaagcagaga cagattggag
     7861 agattcaata tctggataag tccggaaacg caattcgaga gtatcctcag gaaatgctca
     7921 agaagctatt gatagactgg tggcgcgcgc gatttcttcc gctcggttct gaggagattg
     7981 attcctacat caactattca cgctgtctcg atcatgtgaa gaaagactat agaattctat
     8041 acaatgcggg tgtagaggtt cgaaagaagg aacagccgat ggggctctac tctggtttgc
     8101 agagatggat gcgcgaaaat cgcgccagag tgtttggaat tcggcgcatg gaagttttcc
     8161 gtcgttttat tccgggcgtg tttctcaatt tttgattgcg acagcgtggt tttttctgac
     8221 tcttggtcta ccggccggag gccaacatga aacaacttcc acgcattctt cctttcacga
     8281 aagagcatga tgactttcgc gcgatggctg cggatttctt cgatcaagaa gtagctccgc
     8341 accatgaaca atgggagaaa gacggcattg ttccgcgtga aatctgggcc aaggcaggct
     8401 ctcttggttt gatctgtccc aatttccccg aagaatatgg tggagcgggc gctgactttc
     8461 tctataatct gattgtaatt gaggaatccg cgagagtggg agcgagcggt ttcttcatca
     8521 gtctgcacgc agacgtaatt gcaccgtatg ttttgcacca cgcaagcgat gagcaaaaac
     8581 agcgctggct gccagggatc attgacggaa caaagattct ggccattgct atgacggaac
     8641 ctggtgccgg gtcggatctc gcgggcatta ccagtactgc catagacaaa ggggatcatt
     8701 atctggtaaa cggatccaaa actttcattt ccaacggata tctttccgat ttgatcatca
     8761 ctgttgccaa aacagatcct gaaaaagaga tcaacggaat ttcccttctc atggtagaac
     8821 gcggaatgga gggatttgag agaggcaagc gtctgaagaa gattggcctg aatgcgcagg
     8881 atacttctga attgttctat cacgatgtta aagttcctaa agcaaatctt ctgggaaaga
     8941 aaggatatgg ttttcgctat ttgatgagcg agcttgcaac agaacgattg gttctcgcgg
     9001 cgtccagtat gcgcggctgc gaagtggctc tcgaagaaac tctcaaatac gtgaaaaccc
     9061 gaaaagcgtt tgggaaaagt ctgggcagct ttcagaacac tcagttcaaa ctggcagatg
     9121 ctgccacaga agtggcggcc gcgcgttgtt ttctcgatca ggtgatcctg gcgttcatgc
     9181 gaggcgacaa ggtagtcgta gaggcttcgc aggcaaaact actgtgctca gaacttctca
     9241 aaaagacggt ggatacgtgc cttcagttct tcggaggcta cggctacatg atggaatatc
     9301 ccatcgcacg cgcctttgta gacgcgcgcg ttcagagcat ttacgctgga acatcagaga
     9361 tcatgcgcgg gatcattgca tccaagggtc tgggtctctg acgagttcac atccgcttcc
     9421 tgctgaaatc ctggaggaag catacgaact ggcccttcag gcggatctat cgggcgaagt
     9481 tccagtcgga gctctggttg ttgaactctc cactctgaag attgtggggc gcggcaagaa
     9541 tgaaatcatt tcgcgcaaag atccaacggc ccacgcagag ctcctggcta ttcgcgaagc
     9601 atgcgcgcat tttcagtctg aaagaatgct tccgtctgtt cttgtttcta ctcttgaacc
     9661 ctgcacccta tgcaccggtg cgattctgtt tgcgcgcgtt gcggaggttc aatattttac
     9721 gcctgtgctc tcaggggcgg gtattgtgcg attgctcgat caatttggta cttcttacaa
     9781 tcacagacca cttctcactc acatagaaag ccaccaggaa aaagccaggc agattcttgt
     9841 ttcctttttc gagagaa
//
LOCUS       DAORFF010000057         9855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3178_length_9855_cov_4.141122, whole genome shotgun sequence.
ACCESSION   DAORFF010000057 DAORFF010000000
VERSION     DAORFF010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3178_length_9855_cov_4.141122"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKK76_03790"
     CDS             <1..199
                     /locus_tag="PKK76_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNL67615.1"
                     /translation="NFPGYINALRIGRACELMENDPARTILSIAYDVGFSAKSTFNAA
                     FLRHRGMSPTAFRAKLQKRVR"
     gene            377..1171
                     /locus_tag="PKK76_03795"
     CDS             377..1171
                     /locus_tag="PKK76_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNL67616.1"
                     /translation="MNFVRYYPIAGLAFLIFWVLGKKAFALRRVQDSFPKAERIWYEI
                     KYSFGTLVIFTGIGLFSVYLRHFGLTKVYVDLDKYGTWYMIGSFVLLTVWHETWFYWA
                     HRAMHSKLLFKHVHLVHHRSINPTPFAAYSFHPIEAVLEGLYLLIFTMVIPSYRYMVL
                     FHTFYAMLLNIYFHTGYEFFPKNFLRGRITKWINTSTHHNMHHSKTHGNYSLYFNFWD
                     RILGTNHPEYQKTFEEIAGRAADASGGSPSHRPRSGHGVGKEAEAT"
     gene            1244..1831
                     /locus_tag="PKK76_03800"
     CDS             1244..1831
                     /locus_tag="PKK76_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNL67617.1"
                     /translation="MARQFSAQASSELETIIADFPNKKSALLMVLRLAEREFGFLDDS
                     AMELVARSCDVSPAHVLGMVTFYTHFKRPYHGKHRFMVCATLMCSIGGPHGKTDDALQ
                     VLKEKLGLAPGERSEDGLFSVEKVECLADCNQPPVMQKDNEHKVRMGKEELSRYVDDL
                     LKKEGLSAGHYSGKPVVRMDPNVPHISVKQSVPAH"
     gene            1881..2636
                     /locus_tag="PKK76_03805"
     CDS             1881..2636
                     /locus_tag="PKK76_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67618.1"
                     /translation="MTRIARLTIAALLLAEAATPALALGTYAEGQVYLTVRKFESSGI
                     IFESWEGTGELVTVDRSENCKQEENECYTPKKEMISFSVRPETDGGKLAAEIQKNINK
                     EILVAYRIHRIEAAALSTDFEVTGLLEMQDRLAADLPAKKVTRKTGAKRNFSVLGRVV
                     HLEYAGTAVGTYEGIYIDQQKGKAHPFSITDSDMAAHIYKVMALRKDYNLGISQAFVT
                     GVRKSDYDIFEVNMNEPAGAADPQPPETKPDAK"
     gene            2691..4331
                     /locus_tag="PKK76_03810"
     CDS             2691..4331
                     /locus_tag="PKK76_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNL67619.1"
                     /translation="MHGLIEDIGLSIIVATIIGVITHRLKQPIIVGYLLAGIIIGPQI
                     GPQLVHDPEHINIISEIGLVLLLFIIGLEMNPQHLMAGGRAILFAGLGQFPLTVMLGV
                     LLFSAAGFAAGFIDAVYLAFACGLSSTAIVVKALFDKFELDSIPGRLTVGILIFQDVW
                     AILVLALQPNLANPTLAPVLGAILRAVFLLGLGFVFSKYVLSRVFHWISRAPEMVVAV
                     SIGWCAFFAALANQIGLSFEMGALIAGVSISSFPYSEHVTEKILPLRDFFLTLFFISL
                     GMKIPYPQPGLLLTVPVLLGFVVLSRFLIVYPLALLGGSGKRTALIASLNLSQISEFS
                     LVIASIGVARSHISSETMSAILYSLAVASVLSSYVLKYNHQLYGFIVRYAPFFSDSRE
                     SAETSSHAKKDICVLGFHKGARAFVDRIATMKPELLKGMIIVDYSVEVLKEVRDRYSV
                     QILFGDISAADTLERAGVREANYIVSTIPDALLRGTTNQKIVRVCRSLSTEAFIAATA
                     DSRDQAEGLRTEGANQVLLPYVVAGERLAMELVGRHRN"
     gene            4361..5524
                     /locus_tag="PKK76_03815"
     CDS             4361..5524
                     /locus_tag="PKK76_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67620.1"
                     /translation="MLRTTALVLIATLAWCSPEKTGEAKPSQPAVTETPRPGLPELAR
                     FLAGKMEPGESRLGKLAALPGYKQHKTQVSGIWSMYVKDFSVPLQEWREQNVPAVKSK
                     TVFYPFAGPDFPNANTIYPNGETYIMIGLEPAGFAPDFESMSDHKAMSELRRMLQSVD
                     YVSRRSYFITSFMNQSYIITEGVAPVILMYLGLLDFVPYSVKAIDLDPNGKIRYLSDE
                     EVKQYRQQRKWTHVEVRFQAAAGQPLKTIYYFKQDVQDEALEKDPRLLKYLESLPQYA
                     SVFKAASYLIHYETFSRIGDVVVNKSELIVMDDTGPRMKKLQGQFDVTVFGHYGRPIP
                     PFQSQYQSDLAKMYRDQKPQPIKFRYGYGGSDGSRGILLVTRKQQQTQATPEK"
     gene            5548..7821
                     /locus_tag="PKK76_03820"
     CDS             5548..7821
                     /locus_tag="PKK76_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL67621.1"
                     /translation="MIRNPLEFKNAFALFHRTWFDDAKETQEFLRLQGVRRVFLLATV
                     EKPGSPEYTQLNSLRDVLESHGFAVAFATVESVLARPESHKRFLSVLNSSLHKEATAL
                     LYSPAAQQDSIYILSTLLLGAGLSPARALQRIVGEYPDQVLLRRLEVYAAEKNKRKAG
                     NTSQPEERPRFTIRTKLLLMAAGVVVTSLTIVTMLGTFFFKKTSETMIQEYNLSLARL
                     IGLQVQSDLQNIVYRSDDVLAGRGEADFFKQNASVVLVRSGARTVINPEISEDRTAAR
                     EDLIAASSRNGPDVERAVVRGSEFLILRSPSKSTTIVLFASRLLDAFRTTRQTNLFQL
                     YLVDRQGNFLLSTAEFPTTSGREVPIVLQMISSPVDNGSRRYEFQGKEYLGSFQTLNF
                     SGLGIVSFVEADRVFEAVYRIQKQNFKITLIVLAIAFLGVFYFSSTLTGPIVRLVSAM
                     RRVQKGDYHTDIVPKTADEVGILTSSFLSMTRGLEEREKIKDAFGRFVHEEVANMAMH
                     GELKLGGEQRRVAIFFSDLRNFTGIAEKMQPEQVVDMLNQYFSAMVECVYETGGVVDK
                     YIGDAIMAHWGAFRPGSNDTKNAVEAAIRMRESLVRLNQEFARRGQPVLRCGCGINTG
                     DVIAGQIGSRKRLEYTVIGDAVNFASRIEYLNKAFGTDILISHDSMREVEGMFHAVQL
                     PTVKIRGKDEPQIVFAILGRAGDESAPQTLDELRMRLGIQFDAEAARKELHAPSDQIL
                     SDRPAQGSDSGSREARS"
     gene            7818..9329
                     /locus_tag="PKK76_03825"
     CDS             7818..9329
                     /locus_tag="PKK76_03825"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNL67622.1"
                     /translation="MRRIKLNEILVPLICGIVLIVVSISLFQDLTGRSKSEGEVIGFI
                     TYRYRVAQRRAASRVVWEDAEQKDSVYNQDTIRTDEKSEALVTLRNGTMIELDPQSMV
                     VLSMRNDAGEIKLAQGSVVLRKPTSEDRLTFEQGGKTEFIKPTGELRLTLNGKSAEAS
                     GSFEFERKGESFKSERDSIRVEESRVERSRPSFSELFPPDNFRVFTDRDRQDITFTWA
                     GAALNLEVSESRSFTSPLLRQSVSGVNSFKTPLPEGIYYWRILAEGKTSLIHKFRIVR
                     TTEVRLLSPIGNIDLRDDSPFIEFAWSESKLAVSYHLRVATDAQWKNVVLDKEMIRNS
                     GVFPLPPGEYYWQVEAVGALPGSNSSTSIQRFSVKDARVVEKPADKQKEGSASQDVTQ
                     KPQALPDKPEGIVPSPGSVVDMSTRDTLAFQWKPVNGAQRYTIILYQAGGVQVYRVDT
                     KQSMHVLTDLSVLDTGSFAYEIRAYTASGESSSGLIPFSVSLGAQLEKPRLKN"
     gene            9392..>9855
                     /locus_tag="PKK76_03830"
     CDS             9392..>9855
                     /locus_tag="PKK76_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNL67623.1"
                     /translation="MTLEWEPVALAKGYVVQIRTSDNDIKEVRVSENRMEQSLNPGEY
                     QVRLAALNKFGKPGAFTDWITVKIRSRDAGVVRLSRDEKSTDPDRGKPAPPQTVQPGG
                     SEEGRRHVSVWPGALIPGYIQIRGDHPYRGLGEITALSAIAIAFYTERRAGD"
BASE COUNT         2396 a         2359 c         2524 g         2576 t
ORIGIN      
        1 gaatttcccc ggttacatca atgcccttcg cattgggcgc gcgtgcgagc ttatggaaaa
       61 tgatcccgca cgaacgattc tttctattgc ttatgatgta ggtttttccg cgaagtccac
      121 attcaatgcc gcttttttgc gtcatcgcgg gatgagccca actgcatttc gcgctaaatt
      181 gcagaagcgc gtgcgctaaa aacagctccc gaaggcaaaa cgggtccagt gttatgaatc
      241 tggacgcctt ttttgttcca ggtttatact accggtcgat tcgcaaaggc ctctgtggta
      301 tgcttcgatc atgaactttc tacaatgcgg aatcaccctc gactgctttg aaaaggcggg
      361 atcattctta ttgaccatga actttgtgag gtattatcca attgctggcc tcgcattttt
      421 gattttctgg gtgctcggca agaaggcgtt tgccctgcga agagtacagg attctttccc
      481 gaaggcggaa cgaatctggt atgagatcaa gtactctttc ggtactctcg taatctttac
      541 tggcattggc ctgttctcag tctatctcag gcattttggt ctgactaaag tctacgtcga
      601 tctggacaaa tacggaacct ggtatatgat cggctctttt gttttgctga ccgtctggca
      661 tgaaacctgg ttttactggg cacatcgcgc gatgcactct aaactcttgt tcaagcatgt
      721 tcatctggtt caccacagat cgatcaaccc gactcctttc gccgcctatt cgtttcatcc
      781 gatcgaagca gtgttggaag gattgtatct tttgatcttc accatggtga ttccgtccta
      841 tcgttatatg gttttgtttc acacgttcta cgcgatgttg ctcaacattt acttccatac
      901 aggatacgag ttttttccaa agaactttct gcgcggaagg attacgaaat ggatcaatac
      961 gtctacgcat cacaacatgc atcactcgaa gacgcacggt aattacagtc tctacttcaa
     1021 tttctgggat cgcatcctgg gcacgaatca tccagaatat cagaagactt ttgaagaaat
     1081 tgcaggaaga gcagcggatg cgtctggagg ttctccgtcg cacagaccgc gctcagggca
     1141 tggtgtgggc aaggaagcag aggccacctg agcaaagcgc gttccatggc gattggatcg
     1201 gaactgtttg acaaagaaat gagccatcga ttctctgatt gccatggcgc gtcagttttc
     1261 ggcacaggca tcgagcgagc ttgagaccat catcgcggat ttccctaaca agaaatccgc
     1321 tcttctgatg gtgctgaggc ttgcagaaag agagttcggc ttcctggacg attcggccat
     1381 ggaactggtg gcccgttcct gcgatgtcag ccctgctcac gttctgggca tggttacatt
     1441 ctacactcac ttcaagcgtc cttaccacgg caaacatcgc tttatggtct gtgccacttt
     1501 gatgtgttcg attggtggcc cgcacggaaa gacggatgac gcccttcaag tcctcaaaga
     1561 aaaacttggt cttgccccgg gcgagcgttc ggaagacggc ttgttttccg tggagaaggt
     1621 ggagtgtctt gcggattgca accagcctcc tgtcatgcaa aaagacaatg aacacaaagt
     1681 tcgcatgggc aaagaagaac tgtcgcgata tgtggatgat ctcttaaaga aagagggact
     1741 gagcgcgggg cactattccg gcaagccagt ggttcgcatg gatccgaacg tgcctcatat
     1801 atccgtgaaa cagagtgttc ccgcccatta gtcgctcaaa agttacgtga cacatcagaa
     1861 gggtgcttca tacccttccc atgactcgca tcgctagact aacaattgca gcgcttctcc
     1921 tggcagaggc tgctactcct gccctggctc tcggaaccta tgcagaaggt caggtgtatc
     1981 tgactgttcg caagttcgaa agcagcggca ttattttcga atcctgggaa gggaccggcg
     2041 aactcgttac tgtggatcgc tctgagaact gcaagcagga agagaatgaa tgctatactc
     2101 ccaaaaagga aatgatttcg ttcagcgttc gtcctgaaac agacggcgga aagctggcag
     2161 cggaaataca aaagaatatc aataaagaaa tcctggtcgc ctatcgcatt catagaatag
     2221 aagcggccgc tctttctaca gattttgaag tcacaggact cctcgagatg caggatcgtc
     2281 tggcggcgga tcttcccgcg aagaaagtga ctcgcaaaac aggcgctaag cgaaacttca
     2341 gtgtgctcgg tcgcgtggtt catctggaat atgcgggaac tgcagtggga acctacgaag
     2401 gcatctacat tgatcagcaa aagggaaagg cccatccttt ctctatcaca gattctgata
     2461 tggcagctca catttacaaa gtgatggctc tgagaaaaga ttacaatctt ggaatcagcc
     2521 aggcgttcgt aacaggagtt cgcaagtcag actacgatat tttcgaagtc aacatgaatg
     2581 aacctgcggg agcggcagat ccgcagcctc ctgaaacaaa gccggacgct aagtagatcg
     2641 tacgtttcta gttcctttcg acgcgggcaa cagcccgcgt ttctattccc atgcatggac
     2701 tcattgaaga catcggcctt tcgatcatcg ttgcgacgat catcggtgta attacgcaca
     2761 gactgaaaca gccgatcatc gtaggctatc ttctcgctgg aatcatcatt ggtccgcaga
     2821 ttgggccgca gctcgttcac gatccagagc acatcaacat catctctgaa attggtctgg
     2881 ttcttctgct attcatcatt ggcctggaaa tgaatccaca gcatctgatg gcaggaggac
     2941 gagcgatcct gtttgccggg cttggtcagt ttccactgac agtaatgctt ggtgtattgt
     3001 tattcagtgc tgccggcttt gctgcgggtt tcattgatgc cgtttatctg gcttttgcct
     3061 gcgggctttc cagtactgcg attgttgtta aagcactctt tgataaattc gaattggatt
     3121 cgattccagg tcgcctgacg gtaggaattt tgatcttcca ggatgtgtgg gccatcctcg
     3181 tgcttgctct tcaacctaac cttgcaaatc caacccttgc accggttctg ggagcgatct
     3241 tgcgcgcagt cttccttctc ggacttggtt tcgtgttcag taagtacgtg ctttcaagag
     3301 tgtttcactg gatttcgcgc gcacctgaaa tggtcgttgc tgtgtctatt ggttggtgtg
     3361 ctttctttgc ggcactggca aatcaaattg gactctcgtt tgaaatgggt gctctcatcg
     3421 caggcgtctc tatctccagt tttccttaca gtgaacatgt gacggagaag attcttcctc
     3481 ttcgcgattt ctttctgact ctgtttttta tttctcttgg tatgaagatc ccctatccgc
     3541 agcccggtct tttactcaca gttccggtat tgctgggctt cgttgttctc tcgcgcttct
     3601 taattgtata tcctctcgct ctactcggtg gatccgggaa gaggactgca ttgattgctt
     3661 ctctgaatct aagccagatc agtgaattct ctctcgtgat tgcctccatt ggagtggcca
     3721 ggtcgcacat ttcttcggaa accatgtctg cgattctcta tagtctggca gttgcctctg
     3781 tcctttcctc gtatgttctg aaatacaatc atcagttgta cgggttcatt gtacgttatg
     3841 cgccattctt cagcgactcc agggaatcag cggaaacctc ttcgcatgca aaaaaggata
     3901 tctgcgtgct tggctttcac aaaggagcgc gggccttcgt ggaccggatc gctacaatga
     3961 aaccagagtt actcaaagga atgatcattg tagactacag tgtggaagta cttaaggaag
     4021 tcagggaccg ttattcggtt caaatcctgt tcggcgatat ttcggcggcg gatactctcg
     4081 agcgggcagg agttcgggag gcaaactaca tcgtttctac gatcccggac gcgcttttgc
     4141 ggggcactac gaaccagaaa attgtccgcg tatgtagatc cctgtctacc gaggctttca
     4201 tcgcggccac tgctgattcc agagaccagg cagaaggtct gcgaacggaa ggggctaacc
     4261 aggtgctctt gccatacgtt gtagctggcg aacgactggc catggaactg gtcggtcgtc
     4321 atagaaattg actccggcag tccatttcct atcctgggcc atgcttcgca ctactgcact
     4381 tgtattgatc gccactcttg cctggtgttc tcctgaaaaa accggagagg caaagccttc
     4441 gcagccagca gtcacagaaa cacctcggcc cggtctgcca gagctcgcgc ggtttcttgc
     4501 cggcaagatg gagccaggcg aaagtagact ggggaagctt gccgcgctcc ctggatacaa
     4561 gcagcacaag actcaagtct ccggtatctg gtccatgtat gtgaaagact tttccgttcc
     4621 tctgcaggaa tggcgcgagc agaacgtccc ggcagtaaaa tcaaagacag ttttttatcc
     4681 tttcgccggc cctgattttc caaacgcaaa tacaatctat ccaaacggtg aaacgtacat
     4741 catgatcggc cttgagccgg ctggtttcgc gcctgatttt gagagcatgt ctgatcacaa
     4801 agcaatgagc gaattgcgca gaatgctgca gagcgtagac tacgtttcgc gcagatctta
     4861 tttcatcact tcattcatga accagagtta catcatcaca gaaggtgtgg cacctgtgat
     4921 tttgatgtac ctggggcttc tggattttgt tccgtattct gtaaaagcaa tcgatctgga
     4981 tccaaatgga aagattcgct atttgagcga tgaagaagta aaacaatatc gacagcagag
     5041 aaagtggacg cacgtagaag ttcgcttcca ggcggcggcc ggtcagcctc tcaagaccat
     5101 ttactacttc aagcaggacg ttcaggatga agcgctggaa aaagatccac gccttctgaa
     5161 gtatctcgaa tctcttcctc agtatgcgtc tgttttcaaa gccgcttcct acttgatcca
     5221 ctatgagaca ttcagtcgaa tcggtgacgt agttgtaaac aagtcagagt tgattgtgat
     5281 ggacgatact ggtccacgta tgaagaaatt gcagggtcaa tttgatgtga ctgtattcgg
     5341 tcactacggt aggcccattc ctccatttca atctcagtac caatcagatc tggcaaagat
     5401 gtacagagat caaaaaccgc agccaatcaa gtttcgctat ggctatggcg ggtcagatgg
     5461 tagcaggggc attcttcttg taactcgaaa gcaacagcag acacaggcga cgccggagaa
     5521 ataggcgtct gattccgggg agtttctatg atccggaatc cgcttgagtt caagaacgcc
     5581 tttgcgctct tccatcgcac atggttcgac gatgcgaaag agactcagga attccttcgt
     5641 ctacagggtg ttcggcgagt cttccttcta gctacggtag aaaagccagg aagcccggaa
     5701 tacactcagc taaattcgct tcgtgacgtg ctggagtcgc acggatttgc agtagccttc
     5761 gcaaccgtag agtccgttct cgcccgtcct gaaagccaca agcgtttcct gtctgtcctc
     5821 aattcatccc ttcacaaaga agccacagca cttttgtatt cgccggcggc ccaacaggat
     5881 tcgatctaca ttctttctac tttgcttctg ggtgcgggac tttctcctgc tagagctctc
     5941 cagagaatcg tgggcgaata tcccgatcag gttttgcttc gtcgcctcga agtgtatgcc
     6001 gcagagaaaa acaaacgtaa agctgggaat acttctcaac ctgaagagag gccgcgcttc
     6061 accattcgaa ccaagcttct tctgatggcc gcgggtgttg tggttacttc tctcaccatc
     6121 gttacgatgc ttggaacttt cttcttcaag aagacaagcg agaccatgat ccaggaatac
     6181 aatctcagtc tggctcgtct gatcggactt caggttcaat cagatcttca gaatatcgtc
     6241 tatcgttccg atgacgttct tgcaggacgc ggagaagcag attttttcaa acagaatgct
     6301 tctgttgttc tcgtgcgctc cggtgcgcgc acagtgatca acccggaaat ttcagaagat
     6361 cgcactgcgg ccagagagga tttgatcgct gcttcttcta gaaacggacc ggacgtagag
     6421 agagcggtgg ttcgtggcag tgaatttctt attctgcgtt ctccatcaaa atcaaccacg
     6481 attgttctct ttgcgtcgcg attgctcgat gcatttcgca cgacgcgtca aacgaatctc
     6541 tttcaactgt atctggtgga caggcagggg aactttcttc tctctaccgc ggagtttccg
     6601 actacaagtg gtcgtgaagt acctatcgtt ctgcagatga tctcaagtcc agtggacaac
     6661 gggtccagga gatatgagtt tcagggaaag gaatacctcg ggtcattcca aacactgaat
     6721 ttcagcggac ttggcattgt ttcgtttgtg gaggcagaca gggttttcga ggctgtttac
     6781 aggattcaaa aacagaattt caaaattact ctgattgtac ttgcgatcgc atttctgggc
     6841 gtcttttatt tctcatctac tctcactgga cccattgtgc gccttgtatc cgccatgcgc
     6901 cgcgttcaga aaggagacta tcatacagac attgtcccga aaacggcaga tgaagttggc
     6961 atacttactt cctcattctt gtctatgacg cgcggcctcg aagaaaggga aaagatcaag
     7021 gacgcctttg gtcgctttgt tcatgaggaa gtggccaaca tggccatgca cggcgaactg
     7081 aagttaggcg gagaacagcg tcgcgttgcc attttctttt ctgatttgcg gaattttacg
     7141 gggatcgcag agaagatgca gccggagcag gtcgtggata tgctcaatca gtatttttcc
     7201 gccatggttg aatgtgtcta tgaaacaggc ggagtcgtgg ataaatacat aggcgatgcg
     7261 atcatggcgc actggggggc cttcagaccg gggagcaatg acacaaaaaa cgcagtggag
     7321 gcagccattc gcatgcgcga aagcctggtt cgattgaatc aggagtttgc gcgaagaggc
     7381 cagccagtct tgcgatgcgg atgcgggatc aataccgggg atgtgattgc aggacagatc
     7441 ggatcgagaa agcgtcttga gtatactgtg atcggggatg cggtgaattt tgcatctcgg
     7501 attgaatact tgaataaagc ctttggtaca gacattttga tttcgcacga ttctatgcgc
     7561 gaagtggaag gaatgtttca tgccgtgcaa ttgcctaccg taaagatacg cggcaaagat
     7621 gagccccaaa ttgtttttgc aattcttggg cgcgcagggg atgaatccgc cccccagact
     7681 ctcgacgagc tgcggatgcg actgggcatt caatttgacg cggaagcagc gcgcaaggaa
     7741 ttgcatgcac catcggatca aatcctctcg gatcgcccgg ctcaagggtc tgattctgga
     7801 tcgcgcgagg cccgcagttg agacgtatca agctcaatga gattcttgta cccttgatct
     7861 gtggaatcgt cttaattgta gtttcgattt cgctttttca ggatcttacg ggtagaagca
     7921 aatccgaagg tgaggtcatt ggctttatca catacagata ccgcgtggca cagcgcagag
     7981 ccgcatctcg cgtagtctgg gaagacgcag agcagaaaga ttcagtgtat aaccaggata
     8041 caattcgcac cgacgaaaaa tcggaagctc ttgtaacgct gcgcaatggc acgatgatag
     8101 agctggatcc acagagtatg gtggttctgt ccatgcgcaa tgatgcgggc gaaatcaagc
     8161 ttgcgcaagg atctgttgtt ctcagaaaac ctacgagtga agatcgcctg acgtttgagc
     8221 agggtggaaa gacggaattc ataaaaccaa ccggagaatt gcgactaacg ttgaatggga
     8281 aatcggcaga ggcatccggt agctttgagt ttgaaagaaa aggagaatct ttcaaaagtg
     8341 agcgggattc gattcgcgta gaagaaagcc gagttgagag aagcaggccc tctttttcgg
     8401 agctttttcc accggacaac tttagagttt tcactgatcg ggaccgccag gacataactt
     8461 tcacgtgggc tggagcggcc ttgaatctgg aagtaagtga atcacgctca tttacttctc
     8521 cacttctgag acagagcgtt tctggcgtga attcgttcaa aactccattg cccgaaggga
     8581 tctactactg gagaattctt gctgaaggaa agaccagcct gattcacaaa tttcgaattg
     8641 ttcgcactac agaagttcgg ctactttccc caataggaaa cattgattta cgcgatgaca
     8701 gtccttttat tgagtttgcg tggtcggaaa gcaagcttgc tgtttcgtat catttgcgtg
     8761 tggctacgga cgctcagtgg aagaatgtcg tgctggataa agaaatgatt cgcaattctg
     8821 gagtttttcc tcttcctccg ggagaatatt actggcaggt agaggcagtt ggtgcgcttc
     8881 caggttcgaa cagcagtact tccatccagc gattttcagt caaagacgca agggtggttg
     8941 aaaaaccagc ggacaaacag aaagaaggat ctgcttcgca ggatgtaact caaaaacctc
     9001 aagcattgcc cgataagcca gaagggatcg ttccttctcc tggatcggtc gtggacatgt
     9061 ctaccaggga cacacttgcg tttcaatgga aacctgtgaa tggggcacaa cgttatacga
     9121 tcattctcta ccaggctggc ggcgtacagg tctaccgtgt ggatacaaag caatccatgc
     9181 atgttctgac tgatctttca gtgctcgaca ctggctcctt tgcctatgag attcgcgcat
     9241 acaccgcgtc tggagaaagt tcatctggac tgattccgtt ttctgtgtca ctgggtgctc
     9301 agcttgaaaa gccaaggttg aaaaattgaa atcgctcctt tccgttttcg ttgttctgat
     9361 cttttctcta ccgctgcgtg ctgaacagaa aatgactctg gaatgggagc ctgtagcact
     9421 ggcaaaggga tacgtggttc aaattcgcac aagtgataac gacatcaagg aagtgcgagt
     9481 ctcagagaac agaatggaac aaagtctgaa tccgggagaa tatcaggtgc gtctggctgc
     9541 gctcaataag tttgggaagc ccggtgcatt cacggactgg atcacggtca aaattcgctc
     9601 gcgggatgcc ggtgttgtga ggctttcgcg cgatgagaaa tcgacggatc cagacagagg
     9661 aaagcctgcg ccaccacaaa ctgtccaacc cggaggaagc gaagagggac ggcgccatgt
     9721 gtccgtctgg ccaggagctc ttatcccggg ctacattcaa atcagggggg atcacccata
     9781 ccgcggtctt ggagagatta ccgctctctc tgcgattgcg atcgctttct acacagagag
     9841 aagagcagga gataa
//
LOCUS       DAORFF010000058         9806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3203_length_9806_cov_4.623423, whole genome shotgun sequence.
ACCESSION   DAORFF010000058 DAORFF010000000
VERSION     DAORFF010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9806
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3203_length_9806_cov_4.623423"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="PKK76_03835"
     CDS             <1..1076
                     /locus_tag="PKK76_03835"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNL67624.1"
                     /translation="PTLKARKLTARGIPIGANDEDYLGAATRILLRYALTGRPASFRS
                     PEDFFRKTLEQLIAAKTLSMVSVWSPTFLISLDARLREMWADLKKPSSVPARFETWDE
                     IWPNLKMISMWTDASAAAFVPQVRSITGNIPVQGKGLLATEGITSIPILHDMDPVIAY
                     RSHFFEFKNERGIFRLDELQAGESYEVILTTGGGLIRYATGDIVTVTGFMRRVPTIRF
                     KARNEFSDLVGEKISEEAGLAAIHRALKVCHGICGIFLRHADPGAKMGAAGYELLLFV
                     ESVPENAPSVASAATQAATEILEQNPYYTLAIQTGQLRNLRVRFLGRDALEKMQKKLA
                     AQAKREGTWKWPAFTSEAHPDEI"
     gene            1051..2136
                     /locus_tag="PKK76_03840"
     CDS             1051..2136
                     /locus_tag="PKK76_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67625.1"
                     /translation="MKHIQMRFEFREAKPEDNSEICEIFRIPMGGSLALSLERDPDYF
                     AGARVQNEKPRVFVGVDHEKHCLTGSFSVGERRVYLNGKPEWIPYFCDLRIRDEYRSG
                     TLLARGYKFVKEEVLAGRYAQTIIVADNQEAINSLTGSRAGLPTYVPFGAYVTRAIPG
                     NAASKMKYDASISIRKAQMADLGLMKSLCEEAGRQKQFYPCYDFEFSSPYFRDLSFDQ
                     YYIAYRNGSPVGMTGVWNQKSFKKTRICAYGRGLNLARPLYNAWMKLKGGISLPEPGS
                     ILPYSYLHTVNTKNNDPQILRALVREIANDRDDAEYFLLGLDARDRLGDAFRGLSVRE
                     FHGFHYLVDAGATLPEPSLFYLEAARI"
     gene            2184..2912
                     /locus_tag="PKK76_03845"
     CDS             2184..2912
                     /locus_tag="PKK76_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNL67626.1"
                     /translation="MFDPSQNYFVRGLLPAWNYRLVLGSITPAAIEAGRRHSASNQMT
                     CLFGEAMLGAFFLECAGSKSDAIKTCLHLEGTGPAQRIIAFASIEGRVRAHAGVPQAQ
                     WNGSLEEGKGPGFVKVSRWFSDSNIYTSSVEMRLAPIAKNLEDYLTRSDQVQAFVKIQ
                     TIFGPDSEPERISGCMIQALPGASATDTDHALGWLGAAWPDLGREIDYKDGILSKSPF
                     RTLGAGEFQFYCDLWTGYPICCFL"
     gene            2897..3037
                     /locus_tag="PKK76_03850"
     CDS             2897..3037
                     /locus_tag="PKK76_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNL67627.1"
                     /translation="MLLSLGEEAVQEIIKEHGLVEVFCEFCRERYEFRPEQIGTLFGH
                     GK"
     gene            3079..3372
                     /locus_tag="PKK76_03855"
     CDS             3079..3372
                     /locus_tag="PKK76_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67628.1"
                     /translation="MHDVSGRYRFTHMTESGQTVTLMLLLTQVGTDIRGKLRSIFASA
                     GDDQLYYTEEHDVRGNWNEGPIALLLPEGQITVESAPGGLNVTRDQTEFWKSI"
     gene            complement(3383..4633)
                     /gene="nuoF"
                     /locus_tag="PKK76_03860"
     CDS             complement(3383..4633)
                     /gene="nuoF"
                     /locus_tag="PKK76_03860"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNL67629.1"
                     /translation="MAVFEPVLTKHIYESGSETMDHYLARGGYGTAREWIGKEPAEIQ
                     KIVNDSGLRGRGGAGFPTGRKWSLLARGTDKPVYLAVNGDESEPGTFKDRYLLERDPH
                     QVLEGIILTSYAIGSHHAFLYMRGEFIKGYEMMRQALEDAKKKGYIGSNIFGKGYDLK
                     VTLVRGAGAYICGEETGMLTSIEGNRGYPKVKPPFPAVAGLFGCPTIINNVETICDVV
                     HVVKNGAAWHRQWGTEQSPGFKLFCVSGHVNKPGLQEVPLGTPLTEVIANAGGVLPGK
                     KIKAVIPGGSSTPLLTGDKIEKANMDYESIAALGSFLGSGAITVLGDDVDMVRSVFNL
                     MRFYHHESCGQCTPCREGTGWLEKLVHRVYAGHAQKSDLDLINDVCNNMRGRTICALS
                     DAAVMPMTSYLKEFRAEFEARIPT"
     gene            4723..5685
                     /locus_tag="PKK76_03865"
     CDS             4723..5685
                     /locus_tag="PKK76_03865"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNL67630.1"
                     /translation="MRFFLLFLAAISLHAEPLPELKTPVTDLTGTLSKEQISNLENRI
                     LGLEKEKGSQIAIAIVPTTSAENIEQYSIRLATAWKIGRKGVDDGVLLVVAKQDRKLR
                     IEVGYGLEGAIPDAYAKQIIDMTIVPRLREGKFYEGISDGLDRIISLIKTESLPPAQK
                     EKAIPRMSNTEWMLAVLIHGGFWLRPALLLLGVILFIWKRDLKGLVIPLGIWLAWILF
                     WSLRVQAFFFSGLISAAVYSLVYYLFLNRFRSSTGASSSSSGWFSGTSSRSSTYRPTN
                     SSYRSSSSSSSYRSSSSSSSSSSSRSSYSGSGGSFGGGGASGSW"
     gene            complement(5703..6953)
                     /locus_tag="PKK76_03870"
     CDS             complement(5703..6953)
                     /locus_tag="PKK76_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNL67631.1"
                     /translation="MRAFLFVLAFYSTFSGLFAETRKHPSKPIGSIHPGVVHRGNLPE
                     QTVRISGLKAVMLGGNVDGIEGATTVSYRGYLKRVAAVLRARGVQVTEIYSPTKKETI
                     AAAISGAHFIFYAGHGIGSSEPPRYQGDITPAGMLVVDEVWSNAEDVAQWKPAPGAIV
                     FFLGACFTAGNSGDDIGKIQDAEAKRRIGVYSAPFFLRNRFGAYYAAWSDSAAQDVMA
                     ELFAGRTLGQAYDPDGNMSGVFKSQHPNVSSSELWYHRSNYDNRGYAFDYAFVGKPGM
                     TLEQLFKDTSQTTTTTTTTPTTNENKIDPEEAKRKGTELIRAIYDGQDASAIRLINEG
                     ADTNVRYSQWTPLMLAVYYDRPSVVRALISVRADLNAMMDTWTALSLSDAYGRTEISG
                     LLRAAGAKTERAMPGTRPLAPPRR"
     gene            6996..7544
                     /locus_tag="PKK76_03875"
     CDS             6996..7544
                     /locus_tag="PKK76_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNL67632.1"
                     /translation="MRSPARNRTGSYPATEDPPDPQIIACTYNYSGNDGRVKDGNSKY
                     CTCLFFASGALSRSLGRLAEEEFSTTGLAPSYAFLLMAVNEKPGIQPGEIAEEMQLAA
                     STVTRLIDKMESRGFLKRKTTGKKAHIYPTQKSRDLDAQIKEAWASLYKRYTTLLGES
                     TARKLTTATYEADRILEGRKKV"
     gene            7541..8185
                     /locus_tag="PKK76_03880"
     CDS             7541..8185
                     /locus_tag="PKK76_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNL67633.1"
                     /translation="MSKSVGIIGSGGVGKTLGLGFLKQGYNVTIGTRDPAKLKEWKDK
                     AGEKASIGSFADAARCDIVVLATLWTGTKSAIDQAGPDNMKGKVVINVTNPLDFSAGP
                     PPRFAATVENPGGAQVQAWIPGARVVKAFNTISANIMIQPKLEDGTADLFIAGNDEAA
                     KKEVTAIAQAWGWGSVNDMGDILMSFWLETFAMLWIYFGFKNNHWTHAFKLLRK"
     gene            complement(8189..9391)
                     /locus_tag="PKK76_03885"
     CDS             complement(8189..9391)
                     /locus_tag="PKK76_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67634.1"
                     /translation="MPSLLDSIQSGAYADARAQARELVSSDPRALSQSAVKKWAGMRF
                     RNLFISHATSDPAAVSAAVQKKLIGKDSRPDRQKISERFLRLDGPPEWQLEMPEAPAP
                     SLSNNVVLFCPGLLTGLLPVMAFQEAFPAVESKFGTRILQSDSHPMRGCEDNVADIEN
                     ALMKGIGLDAHAKKIVAGNEKIPGSAMAICYSKGMPDLLTALVRVPELRKKIRCIFNW
                     AGAPGGSPLADNIYNSVKDVDITVPEGLTGVLQVISPLLRIDEKVPRFSEYRVKEAAR
                     SLTTTERGAFLDRHLDEIDSWNIPVFNITGSTTVMEVPYFQMQGVMELNKYDANNDMQ
                     VTQNQAKLRTPMATDLAMMHGHHWDLSYSPFPPNMRFGAQHLDHPFPKESALVAMIQL
                     ALELGLVD"
     gene            complement(9435..>9806)
                     /locus_tag="PKK76_03890"
     CDS             complement(9435..>9806)
                     /locus_tag="PKK76_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNL67635.1"
                     /translation="PFGKGRAHIGWQMMHCAATLDRYLNVRILEKDPVDSHLVTNYAG
                     GSSPDDQVRITPDQIRSALAATAPPFLSYFRALSEDRLSQKPHERADRTHVEIFHLLT
                     WHEAHHQGQCHLAMNCFMAGR"
BASE COUNT         2467 a         2498 c         2504 g         2337 t
ORIGIN      
        1 cgcccacttt aaaggcgagg aagttgaccg ccagaggaat tcctatcggc gcaaacgacg
       61 aggactatct gggagctgcc acgcgcattt tgctacgtta tgcgctcacg ggaaggcccg
      121 cttctttccg ctcgccggag gatttctttc gaaagaccct tgaacagctg attgcagcaa
      181 agaccctgag catggtgtct gtgtggagtc caaccttcct catatctctc gatgcgcgtc
      241 tacgagagat gtgggcagat ttgaagaaac catcatctgt tccggcacga tttgaaacct
      301 gggatgaaat atggcccaat ctaaaaatga tcagcatgtg gacagatgct tccgcggctg
      361 catttgtgcc gcaggttcgc tccatcaccg gcaatattcc tgtgcaagga aaaggacttc
      421 tggctacaga gggaatcaca tcgatcccga ttctgcatga catggatccg gtgatcgcct
      481 atcgttctca cttttttgaa ttcaagaatg aacgcggcat ttttcgtctg gatgaactcc
      541 aggccggtga atcatacgaa gtcatactca ctacaggagg cgggctgatt cgatatgcaa
      601 caggcgatat agtgactgtc actggattca tgcgtcgtgt gcctacgatt cgtttcaaag
      661 ctagaaatga attttcagat cttgtgggag aaaagatctc cgaagaagct ggcctcgcgg
      721 cgattcatcg ggcgctcaag gtctgccatg gaatttgcgg aatttttttg cgccacgcag
      781 accctggcgc gaagatggga gcggccggat acgaattact tctttttgtg gagagcgtcc
      841 ctgaaaacgc gccatccgta gcgagtgcag caacgcaggc tgccacggaa attctcgagc
      901 aaaaccccta ctacaccctt gccatccaga ccggccagct gcgaaacttg cgtgtacgct
      961 ttctgggtcg cgacgcgtta gaaaaaatgc aaaagaagct ggcggcacag gcgaaaagag
     1021 aaggaacttg gaaatggccc gcgttcacaa gtgaagcaca tccagatgag atttgagttt
     1081 agagaagcaa aaccggaaga caattcggaa atctgcgaaa tctttcgcat cccgatgggc
     1141 ggatctctgg cactttcact ggaaagagac ccggactact ttgctggcgc gcgcgttcaa
     1201 aacgaaaagc cgcgtgtctt cgttggcgta gaccatgaga agcactgcct cacaggaagc
     1261 ttcagcgtgg gagaacgccg tgtttacctc aacggaaaac cagaatggat tccgtacttt
     1321 tgcgacctca gaattcgaga cgaatatcga agcggcactt tgcttgcgag aggatacaaa
     1381 ttcgtcaagg aagaagtcct ggctggacgt tatgcgcaaa ccatcatcgt ggctgacaat
     1441 caagaagcaa tcaattctct caccggaagt agagcggggc ttccgacgta tgttccattt
     1501 ggcgcatatg ttacgcgagc gatcccggga aacgcagctt caaaaatgaa atatgacgcc
     1561 agtatttcca ttcggaaagc acaaatggca gatttaggcc tcatgaaatc gttgtgcgaa
     1621 gaggccggca gacagaaaca gttctatccc tgttatgact ttgaatttag ttcaccttat
     1681 ttcagagatc tttcttttga tcagtactac atagcatata gaaatggatc accggtggga
     1741 atgacgggag tctggaacca gaagtcgttt aagaaaacca ggatttgcgc ctacggacgc
     1801 ggattgaatc ttgcaaggcc tctttacaac gcatggatga aactcaaggg gggaatctct
     1861 cttcccgaac ctggttctat tctgccttat tcctacctgc atactgtaaa tacgaagaat
     1921 aatgatccac aaattctaag agcgctcgtg cgcgaaattg cgaatgaccg ggacgacgca
     1981 gaatactttc tacttggtct ggatgcgcga gacagactgg gagatgcttt caggggcttg
     2041 agcgtacgcg aattccacgg ttttcactat ctagtggatg ccggtgccac acttccagaa
     2101 ccttccctct tttatttgga agccgcgcga atctagaaat caattcttga ttgaatcggg
     2161 agggtggcgc attacaaagg cacgtgttcg atccctccca gaattatttc gtacgcggac
     2221 ttcttcctgc ctggaattat cgacttgtgc tcgggtccat tacgccggca gcaatagaag
     2281 caggccgcag acattctgca tccaatcaga tgacatgcct gtttggagaa gcaatgctcg
     2341 gtgcgttctt tcttgagtgt gcgggatcca aatcagacgc cattaaaaca tgccttcatc
     2401 tagaaggaac cggacctgca caaagaatca ttgcttttgc atccattgaa ggacgcgttc
     2461 gcgcacacgc tggagttcct caggcacaat ggaatggctc cctcgaggaa ggaaaagggc
     2521 cgggattcgt aaaggtcagt cgctggttct cagatagcaa tatatacaca tcgagcgtag
     2581 aaatgcgcct tgcccccatc gcgaagaatc ttgaggacta tctcactcgc tcggatcagg
     2641 ttcaggcatt cgtgaaaata caaacgatct ttgggccaga ctctgaacca gaaagaatct
     2701 caggttgcat gatccaggct ttgcccgggg cttctgcgac ggatacggat catgcacttg
     2761 gctggctcgg tgctgcatgg ccggatctgg gtcgagagat agattacaaa gatggaattc
     2821 taagtaaatc tccatttcgc actctaggtg ccggggaatt tcaattctac tgcgatcttt
     2881 ggacagggta tccaatatgt tgctttctct aggcgaagaa gcggttcagg aaatcatcaa
     2941 agaacatgga ctggtggaag tattttgtga gttctgtcgc gaacgatacg aatttcgccc
     3001 ggagcagatt ggaacgttat tcggccacgg gaagtgaaaa atcaattgtc caggactgaa
     3061 ggccggggag aattgttcat gcacgatgtg agcggaagat atcgtttcac gcacatgacc
     3121 gaatccggcc agacggtgac actcatgctt cttttgacac aggtggggac agacattcgc
     3181 ggcaagcttc gttctatttt tgcctctgca ggtgatgatc agctctatta cacagaagag
     3241 catgacgtac gcggcaactg gaatgaaggg ccaatagccc ttttgctacc ggaggggcag
     3301 attacagtgg agtctgctcc aggtggactg aacgttacac gggatcagac ggaattctgg
     3361 aagtccatct agcgcgcagc ccttacgtag gaatgcgtgc ttcaaactcc gcacgaaact
     3421 ctttgagata actggtcata ggcatgactg cggcgtcaga aagagcgcag atggttctcc
     3481 cacgcatatt gttgcacacg tcgttgatca aatcgaggtc tgacttttgc gcatgaccag
     3541 catatacacg atgaaccagt ttttccagcc agcctgttcc ttctctgcat ggagtacact
     3601 gtccgcagga ttcgtgatga tagaatcgca tcaaattgaa aacggaccgg accatatcca
     3661 cgtcatcgcc cagaaccgtg atcgctcctg atccaaggaa tgatccaagc gctgcaatgc
     3721 tttcatagtc catgtttgct ttttcgatct tgtctccggt tagaagcgga gtactcgatc
     3781 caccaggaat cacagctttg attttcttcc ccggcagcac tccgcctgca ttcgcaatta
     3841 cttctgtcag tggtgttccc agaggcactt cctggagtcc tggtttgttc acgtgaccgg
     3901 acacacaaaa gagcttgaac cctgggctct gttccgttcc ccattgcctg tgccacgcgg
     3961 ctccgttttt cacaacgtga accacatcac aaatagtttc aacattgttg atgatggtag
     4021 ggcatccaaa caaaccagcc acagcaggaa acggcggctt cacttttgga tacccgcgat
     4081 tcccttcaat ggaagtgagc attcccgttt cttctccaca aatgtaagca cccgcaccac
     4141 gaaccagcgt gacttttaga tcgtatcctt ttccaaagat attgcttcca atgtatcctt
     4201 tcttcttagc gtcttccaga gcctgacgca tcatttcata gcccttgata aattctccgc
     4261 gcatgtagag aaacgcatga tgcgaaccaa tggcgtatga cgttagaata attccttcga
     4321 gcacttgatg tggatccctt tccaggagat atctatcctt gaaagtcccg ggctctgatt
     4381 cgtctccatt gactgcgagg taaaccggct tgtctgtgcc acgcgcaagt aagctccact
     4441 ttcttccagt tggaaatcca gccccaccac gtccgcgaag acctgagtcg tttacaatct
     4501 tttgaatctc agccggctct tttccaatcc attcacgcgc agtgccgtaa ccgccccgtg
     4561 ccagataatg atccattgtc tcggacccgg attcgtagat gtgctttgtc agaaccggtt
     4621 cgaatacagc catgtttcag gagggaaaag ggccgcccct ggagtgcaag cagaaacctg
     4681 cgaaatgatg gacaggctgt tctcaatatg cctccaatgc tcgtgcgttt cttcctcctg
     4741 tttttagctg caatttctct gcacgcagaa cctcttccgg agctgaaaac gcccgtaaca
     4801 gatcttacgg gcactctttc gaaggagcaa atctcaaacc tggaaaacag gattctcggc
     4861 ctggaaaagg aaaagggaag ccagatcgcc attgcaattg ttccgaccac atccgcggaa
     4921 aacatagaac agtattcgat ccggctggcc actgcatgga aaatcggcag gaagggtgta
     4981 gacgatggag tgctgctggt tgtagccaaa caggatcgta aactgcgcat agaagtgggc
     5041 tacggactgg aaggtgccat tccggatgca tacgcgaaac aaatcataga catgacgatt
     5101 gtgccgcgcc tccgcgaagg aaaattctat gagggcatca gcgacggact cgatcgaatc
     5161 atttcgctga tcaaaacgga gtctttgccg ccagcgcaaa aagaaaaagc aattccgcgc
     5221 atgtcgaata cagaatggat gcttgcggtc ttgattcacg gcggattctg gctgcgacca
     5281 gcgttgcttc tactcggcgt gattttgttt atctggaagc gtgatttgaa agggctagtg
     5341 atcccgctcg gcatctggct tgcctggatt ctgttctgga gtctgcgcgt gcaggcgttc
     5401 tttttttccg gcttaatttc cgccgctgtc tactctctcg tctattactt atttctcaac
     5461 cgttttcgct cctccacagg cgcctcttct tcatcttctg gatggttcag cggaacatcc
     5521 agccgcagct ctacatacag accaactaac agcagttaca gatccagttc cagttcaagc
     5581 agctaccgtt ctagctctag ctctagctca agcagttctt cgcgttccag ttacagcgga
     5641 agcggtggaa gctttggggg cggaggagca tcgggcagct ggtgaagatg cccgattcac
     5701 attcaacgtc tgggtggcgc cagtggacga gttcctggca tcgcgcgctc agtctttgca
     5761 ccggctgcgc gaagcaaacc tgaaatctca gttcttccat acgcgtccga cagagaaaga
     5821 gcggtccacg tatccatcat cgcatttaaa tctgcgcgaa cagaaatgag tgcgcgcact
     5881 acagaagggc gatcgtagta cactgcaagc atcaagggcg tccactgact gtatcgaaca
     5941 tttgtatccg ccccttcgtt gatcaggcga atcgcgcttg cgtcctgacc atcgtagatg
     6001 gcgcgaatga gttctgtacc tttgcgcttc gcttcttctg gatcaatctt gttttcattc
     6061 gtggtcggag tcgttgttgt ggttgttgta gtttgcgacg tatccttgaa caactgctcc
     6121 agagtcatgc ccggcttccc aacaaatgca tagtcaaacg cataaccacg gttatcgtag
     6181 ttggaccgat gataccacaa ttcgctgctc gacacattcg gatgctgaga cttaaatact
     6241 cctgacatgt ttccgtctgg atcgtatgcc tgtccgagag tgcggccagc aaacagttca
     6301 gccatcacat cctgggccgc tgaatctgac cacgctgcat agtaggcgcc aaagcggttg
     6361 cgtagaaaga atggagcaga atacactccg atcctgcgct ttgcttccgc gtcctgaatc
     6421 tttccaatat catcgcctga atttccggct gtaaaacaag cgcccagaaa gaatacaatc
     6481 gctcctgggg cgggcttcca ttgtgctacg tcctccgcgt tgctccatac ttcgtctacg
     6541 accagcattc cggcgggagt gatgtctcct tgataacgag gtggttcgct cgatccgatt
     6601 ccatgacctg cgtaaaaaat aaaatgtgcc ccggaaatcg cggccgcgat cgtttccttt
     6661 tttgttggac tgtaaatctc tgtcacctgc actccgcgag cgcgaagaac tgcagccaca
     6721 cgcttgagat atccgcgata gctcaccgtg gtcgcgccct caattccatc cacattccca
     6781 ccgagcatca cagcctttag cccggaaatc cgaaccgttt gttccggaag attgccgcga
     6841 tgaaccacac ctggatgaat cgatccgatg ggtttgctcg gatgcttgcg agtctccgca
     6901 aacagacccg agaaggtaga atagaaggcg agaacgaaca gaaaagcacg cataccagat
     6961 ggacgagggc cagcgcatag cgggcaagtt tttttgtgcg atcgccagcg cgaaaccgca
     7021 cgggtagcta ccccgccacg gaagatccgc cagatcctca aataattgct tgcacataca
     7081 actattccgg aaacgatggc cgcgtgaaag atgggaactc aaagtactgc acgtgtctct
     7141 ttttcgcaag cggcgcactt tcgcgcagcc tgggcaggct tgcggaggag gaattctcca
     7201 ccacaggcct cgctccttcg tatgcattct tgctgatggc agtcaatgag aagccgggca
     7261 ttcagccggg agaaatcgca gaagaaatgc aactcgctgc atccacagtc actcgactca
     7321 tcgacaagat ggaaagccga ggattcctga aacgaaagac cacaggcaaa aaggcacaca
     7381 tctatccaac gcagaaatcg cgggacctgg acgcacagat caaagaagcc tgggcgtctc
     7441 tatataaacg ttatacgaca ttgctcgggg aaagcaccgc aaggaagctg accacggcaa
     7501 catatgaagc ggatcgaatt ctagaaggga gaaagaaagt atgagtaaat cagttgggat
     7561 cattggttca gggggagtcg gcaaaacact gggtctggga tttctgaaac agggttataa
     7621 cgtcactata ggaacgcgcg acccggccaa actaaaagag tggaaagaca aggcgggcga
     7681 aaaagcgtct atcggcagtt tcgcggacgc agccagatgc gacattgtag tgctcgctac
     7741 attgtggact ggaaccaaat cggcgattga tcaggcgggc cccgacaaca tgaaagggaa
     7801 agtggtcatc aacgtcacca atccattgga tttctccgca ggcccgcctc ctcgctttgc
     7861 cgcgactgtg gaaaatccgg gaggagcaca ggtgcaagca tggattccgg gagcgcgtgt
     7921 agtgaaagcc ttcaacacga tcagcgcaaa catcatgatc cagccaaagc tcgaagatgg
     7981 aacagccgat ttgtttatcg ccggcaatga tgaagcggcc aagaaagaag taactgcaat
     8041 tgcacaagcc tggggctggg gcagcgtaaa tgacatggga gatattctga tgtcattctg
     8101 gctcgagacg ttcgcgatgc tctggatcta tttcggattc aagaacaatc actggacgca
     8161 cgctttcaag cttctacgaa agtagaaatc aatctacgag accaagctca agcgcaagct
     8221 ggatcatcgc cacaagggct gattccttgg ggaatggatg atccagatgt tgtgcaccaa
     8281 accgcatgtt aggcggaaac ggactataag acagatccca gtggtgcccg tgcatcatgg
     8341 ccaggtctgt tgccatgggt gttctgagct ttgcctggtt ttgggtcacc tgcatgtcgt
     8401 tgttcgcgtc atatttgttt agctccatca cgccctgcat ttgaaaatac ggaacttcca
     8461 taacagttgt agatcctgta atattgaaaa caggaatgtt ccaggaatca atttcatcca
     8521 ggtgacgatc gagaaatgct ccgcgttccg ttgtggtgag ggatctcgcg gcttctttca
     8581 ctctgtactc gctaaatcgc ggtacctttt catcgatacg aagaagagga gaaatcacct
     8641 gcaatacgcc tgtcagtcct tccggcactg tgatgtccac gtccttcact gaattgtaaa
     8701 tattgtctgc cagaggcgaa ccgcccggag cgccggccca gttaaaaata caacgaatct
     8761 tctttctcag ttcagggacg cgaacaagag cggtgagtag gtccggcatg cctttggaat
     8821 agcaaatggc catcgcggag cctggaattt tctcatttcc tgccacgatt ttttttgcgt
     8881 gcgcatccag accaatgcct ttcatcaaag cgttttcaat gtccgccaca ttgtcttcgc
     8941 atccgcgcat tggatgagaa tcgctctgga gaatgcgagt tccgaatttt gattcaacag
     9001 cgggaaacgc ctcttgaaaa gccatcacag gaagaaggcc tgtaagaagc cccggacaaa
     9061 aaagtacaac gttattcgac aatgagggtg ccggtgcttc tggcatttca agttgccatt
     9121 caggaggtcc atcgaggcgc agaaagcgct cggagatctt ctgtctgtct ggccgggagt
     9181 cttttccgat taacttcttt tgtacggccg cagagactgc tgcaggatcg gatgttgcat
     9241 gcgatatgaa taaattgcga aatcgcatcc ccgcccattt cttgacggct gactgagaga
     9301 gagcccgagg atcagacgaa acgagttcgc gggcctgggc ccgcgcatct gcgtacgcac
     9361 cggactgaat ggaatcgagg agtgatggca taccggactg taaggcattt ccgtccggag
     9421 acaagcattt tccgttatcg acctgccatg aagcagttca tggccagatg gcactgcccc
     9481 tggtgatgcg cctcatgcca cgtaagcaaa tgaaatattt ccacatgagt tctgtccgct
     9541 ctttcgtgcg gcttctgaga tagcctgtcc tccgaaaggg cgcgaaagta agatagaaaa
     9601 ggtggagctg ttgctgccag tgcagatctg atttgatccg gagtaatgcg cacttgatca
     9661 tccggactgc ttccacccgc atagtttgtg accaggtgcg aatctaccgg atctttttcc
     9721 agaatgcgaa cattcaagta acgatcgaga gtggccgcgc agtgcatcat ctgccagcca
     9781 atgtgagccc gtcccttccc aaaagg
//
LOCUS       DAORFF010000059         9806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3204_length_9806_cov_3.406010, whole genome shotgun sequence.
ACCESSION   DAORFF010000059 DAORFF010000000
VERSION     DAORFF010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9806
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3204_length_9806_cov_3.406010"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1124
                     /locus_tag="PKK76_03895"
     CDS             138..1124
                     /locus_tag="PKK76_03895"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL67636.1"
                     /translation="MAAQRIPKFAYAVNTGSSTISIYTVNPTTGLLSSAGSSATASGP
                     LGIAVDATGKFVYVANQSANLLSFYTIQSTTGAISAVGTIPAGTGPNSIAVDPATKFA
                     YATNQSSNDVSTYTLNGSTGVLTLSGTIAAGSTPWSIAVDPLGRFAYVPNITSNNVSM
                     YTINAVTGVLSANGSVAAGSTPVTVAVDPSGRFAYTANQGSNDVTMYAINQGTGALTS
                     LGNISAGTGPRAIIVDPSGKFVYAANQSSNDITIYQMNTVTGLLSIFATTPGLSSPMG
                     LSVEPSGNFLYVANNTANNLAMFMIDKVTGTLTLIGTVPAGTNPGAISVASY"
     gene            complement(1185..1982)
                     /locus_tag="PKK76_03900"
     CDS             complement(1185..1982)
                     /locus_tag="PKK76_03900"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF022036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-class carbonic anhydrase"
                     /protein_id="HNL67637.1"
                     /translation="MVAVAVGSLATVALIANDDQPPETGPACENFGPQTPRDIDNKHG
                     ENKQTFQTARDYKEMNLCNIHFHNGAEHKSRAFAMESTAGEHGHGGGLACAISKSLSQ
                     AEMKKPAQEICKGLKPGDTIEVHWVHTSCKVKPGKGLGSCMSASCANPELRVESQVFT
                     LVNDPTAMKFGDFGYGGNIVNGFHQARGIPSNTGIPVQFMGSTTGPKYNDHICSPLQV
                     TWSVRPECAKLDINSLGAWCAQNEFEEDHGHGVRPLIVNPKLFAPIK"
     gene            complement(2305..2574)
                     /locus_tag="PKK76_03905"
     CDS             complement(2305..2574)
                     /locus_tag="PKK76_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67638.1"
                     /translation="MTSIGCTVLRDSHVVLSRGRGRLQVAGLEYPQDKRGQERMQATA
                     ASFQSLEQSLDPEIPLVLLNHHPSDFSFLQNQAVDLILSGHTHGG"
     gene            2751..3137
                     /locus_tag="PKK76_03910"
     CDS             2751..3137
                     /locus_tag="PKK76_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67639.1"
                     /translation="MIKKTILPIVLASAWISISEFVRNTFLLHSYWLDHYKNLGITFP
                     EQPINGAIWGLWSLVFAIVIFILAKKFTLVHTTILSWIIGFGLMWLVIGNLGVLPTGI
                     LILAFPLSVLESFLASLIIKKLSADS"
     gene            complement(3183..4160)
                     /locus_tag="PKK76_03915"
     CDS             complement(3183..4160)
                     /locus_tag="PKK76_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67640.1"
                     /translation="MKQEVKQIFEMVRAGRLTDDQAARLMTEIQSKETRSEKNDGSLG
                     SMLRNIGADVGALSSSVGGLYASSLKENDFSMSRVQAPAGKSFVFEGNSIRMSSLGGL
                     HLEDAEFSGNEIHSSSIQDLVLKSSKFSGTSLTATSMENTELDKANAKSLEISSSSLK
                     KIRIQDSEITQARMVASALKDLSFAENSSWSGVFDKSQISSLTLKKSQMADFEVLASQ
                     VSQVRFEETEGMQVLIRMLKMTGTSFLKCKMSDVLFSGGEKWRKSGFKHVTFEIVSME
                     KVLFSDCNLDDTVIKNVNLKDVRILNANLRGKTIESTEEFLKLCETKAA"
     gene            complement(4166..4555)
                     /locus_tag="PKK76_03920"
     CDS             complement(4166..4555)
                     /locus_tag="PKK76_03920"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HNL67641.1"
                     /translation="MSEGIDSSLACPSCRGPLRPRILGCDVCGIKVEGPFVLNEFATL
                     SPEDLHFLRVFVKSEGRVREMQAALGLSYPTVRARLSALKDRLSDDAPAKSVNSPTEE
                     ILSRLSEGKINFEQAMKEIKALPPKKE"
     gene            4810..5460
                     /locus_tag="PKK76_03925"
     CDS             4810..5460
                     /locus_tag="PKK76_03925"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNL67642.1"
                     /translation="MGRLIWTLSLWIVCASAGTCGQYPTSMPWLGLLVAKNNSSASTS
                     PPIVATPQWKIFVTAGVSSPYIGVSGFDADCMGDTNKPADSSVYKAMIVEGTTRRACS
                     TPMCGGGAAEGIDWVLKPSHRYVRASDSADLFTAGSAAVFSFGTWSNPPDAAALSFWT
                     GLTASWTNSPSNCLSWANNLGGTFGATGNSSSTNSSVISAAALDCTGSYHLLCVEQ"
     gene            5611..6336
                     /locus_tag="PKK76_03930"
     CDS             5611..6336
                     /locus_tag="PKK76_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67643.1"
                     /translation="METLPNYGLANTITGFATLFSGLLPLCICYLVERHPGRWMFVYW
                     LIVITGIFTITLHGFGETNPMILERWVWSFLDTGSNIVVVWGIVLAVLEDGYSERIRI
                     RARPAATVLMLVGIVWHFFDRMTSGGYLISFGNWGGFRPGQTWLILFSFAATALFYAK
                     RETIPQKARPLLSLTTVIFVAGLLMATARNDTIVFPFLSLHALWHLTAAFGFISLWAF
                     NDVRFRATPRNRDVSRHSNQDLQ"
     gene            complement(6305..7150)
                     /locus_tag="PKK76_03935"
     CDS             complement(6305..7150)
                     /locus_tag="PKK76_03935"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNL67644.1"
                     /translation="MDSTNLTPLEMAKAVFLFDFTFWLVRYAVFAALAFAFVYFIARQ
                     KFGSRAIQKTNPSRDRLWKEVGWSVSTLFVFALMAILVVLLMRAGLTRRYNSISDYGW
                     GYYLLSWILFILAHDAYFYWAHRFMHWKPIFRYVHAVHHRSTNPSPLAAFAFHPLEAV
                     VEYAFFIPMIMILPLHISITHTYPSIMLLLNVYAHLGYEFLPRAFASHPIGRWVITAT
                     LHNQHHKKFRVNYGLWFYWWDRWFGTIDPAYETEFRANEGPVRQKLENLQTRHVTVGP
                     GSNAG"
     gene            complement(7266..8372)
                     /locus_tag="PKK76_03940"
     CDS             complement(7266..8372)
                     /locus_tag="PKK76_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNL67645.1"
                     /translation="MLRWTSIDFFIAGTLIGATLAIVIGMARLLKHPSTQSDRILGVL
                     FILLSFPQLSIAFERLGLFFIYPRALFPHVPFYVFFGPLAYFYFHQVIEPGFRPVQKK
                     HQLLHLVPFAIVQIALLPLYFLSDAELDRMAHGRCTFQWYLDLIFVGISVYTLIFLAL
                     VLRDFFSLLQNRNGQLPPEYKLTAVFVFLGAVFVSLLVWNQIQRSQMATTLGFLGLGF
                     LPVLMFWGAQRFPQYLDRMKKEASRARYERSRLGGLDSDALIRRLTELMEIEALYSDE
                     DLDLARLAAILDLTPHKLSEILNDKIGLTFKNYVNGYRVRAACELLVQEPDRAVLSIM
                     GAVGFGSKSSFNAEFVKATGQSPSDYRKNLTRQA"
     gene            8506..9294
                     /locus_tag="PKK76_03945"
     CDS             8506..9294
                     /locus_tag="PKK76_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNL67646.1"
                     /translation="MIKHWPPNVRPNYEAGGSAGDAWNLCCDLPRTTELRSMNPAQEI
                     LKLLEKQAGEIPESVRKSFIAGARELVVPSGAQIAFENEACARLPFVLSGGPRVYKAA
                     PDGRSITLYDIDPGETCVLTMSCILGGEPFPAGAEALGETRVLVVSSEDWLRWVESSP
                     SLRRYSHQIAARRLSAVIETLSEVAFHRVNQRVARLIVRRSTGSGMQATHQQIADELG
                     TAREVVSRILKEFERYGWIRMSRHEIALLDKSALVKLAEDADAV"
     gene            9367..9570
                     /locus_tag="PKK76_03950"
     CDS             9367..9570
                     /locus_tag="PKK76_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNL67647.1"
                     /translation="MKKNMGTIDRALRVVAALAVGVLWYLGVISGTVAIVLGVLAVVF
                     VLTSLISFCPLYLPFGLSTRGKE"
BASE COUNT         2335 a         2608 c         2414 g         2449 t
ORIGIN      
        1 ggatgaaagg cttattctgc tctggaatga tcgcgatcct cagtcttgcg aagtgcaatt
       61 tttccgcggc atgcctgcca gcagacccgg ggtgcagtgc tgtgtctttg ccattgtttt
      121 tgtccggaag aatcactttg gccgcccaaa gaatcccaaa gtttgcatac gcagtcaata
      181 caggtagctc caccatctca atatacactg tgaatccaac gacaggattg ctctcctcgg
      241 ctggcagcag cgccacagcc tccggaccac tcgggatcgc cgtagatgcc acaggcaagt
      301 tcgtgtatgt tgccaatcaa tcggccaacc ttctctcctt ctacacgatc cagtcaacca
      361 ccggggcgat ctctgcagtt ggaacgattc cggctggcac aggccccaac agcatcgccg
      421 tagatcctgc taccaaattc gcttacgcaa ccaatcagag ttcgaacgat gtctcaacgt
      481 acactttgaa tggatcgacc ggcgttctca ctctttcagg aacgattgcc gctggttcga
      541 cgccctggtc gatcgcagtg gatcctctcg gacgatttgc ttatgtgcca aacatcacgt
      601 cgaataacgt ttctatgtac acgatcaatg cagtcacagg agttctttct gcaaacggaa
      661 gcgtggccgc tggatcgacg ccggtcacag tggctgtgga tccttccggg agattcgcgt
      721 acactgccaa ccagggttcg aatgatgtta ccatgtatgc tatcaaccag ggaacaggcg
      781 cgctgacttc actcggaaac atttcagccg gcacaggtcc cagagccatc atcgtagatc
      841 catcgggcaa atttgtgtac gctgcaaatc aatcctcgaa tgacatcacg atctaccaga
      901 tgaataccgt cacgggactc ctgtcgatct tcgccacgac gccgggactc tcctctccca
      961 tgggtctgag tgtagaacca tcgggtaatt ttctatacgt ggccaacaac acagccaaca
     1021 atctagccat gtttatgatc gacaaagtga ctggcacgct cactctcatt ggaacagtgc
     1081 cagcaggaac caatccgggt gcgatatccg tagcttctta ctaggctgat cttcgccccg
     1141 gcgtccaatt ggtcagttga ttcgcgtaat acctgttagt ctctctactt gataggagcg
     1201 aatagtttag ggttcacaat gagtggacga actccgtgcc cgtgatcttc ttcaaattca
     1261 ttctgagcgc accatgcacc caggctgttg atatccaatt tcgcgcactc aggtcgaacg
     1321 ctccatgtga cctgtagcgg agaacaaata tgatcattgt attttggccc tgtcgtagaa
     1381 cccataaact ggaccggtat gccggtattg ctcggaatcc ctctggcctg atgaaagccg
     1441 tttacgatgt tgcccccata accaaaatcg ccgaacttca ttgctgttgg gtcattgaca
     1501 agcgtgaaga cctgggattc aacccgcaac tcaggattcg cgcaagatgc ggacatacag
     1561 gaacccagac ctttaccagg tttgaccttg cacgaagtgt gcacccagtg cacttcaatc
     1621 gtatcgcctg gttttaggcc tttgcaaatt tcctgcgcag gctttttcat ctcggcctgg
     1681 gacagagact tgctgattgc gcatgcgaga ccgccgccgt gcccgtgttc gcccgcggta
     1741 gattccattg cgaacgctct ggacttgtgc tctgcgccgt tgtgaaaatg aatattgcag
     1801 aggttcatct ctttgtagtc ccgggctgtt tgaaaagtct gcttattttc gccgtgcttg
     1861 ttgtcgatgt cgcgcggcgt ttgtgggcca aaattctcac aggccggccc cgtttccggt
     1921 ggctgatcgt cgttggcgat caatgcgact gtcgccagcg aaccaactgc tacggcgacc
     1981 atcgccagcg cccacatcct tgacttcttc cctatgtgca tcgtaacctc ccgcagcatt
     2041 gctgaggtca cgttttctat cgctcggact tgtccgtcaa cccttcgatt cgcttctcaa
     2101 acactgcggc aacgatgatg gctaacgcaa gacgtgaatc gtaatttcgg gggaaacacc
     2161 ggagcgcaca ggaatggaat gccctagacc cctgtttaca tacaacttgc accggccacc
     2221 aggagaatcg gaaaagtacg caccgctgta atacggatat cggtctggca tcgcgcggag
     2281 aacgttccac ccggcgatct cgatctagcc tccatgtgta tgcccggaaa gaatcagatc
     2341 tactgcctga ttctgaagga aggaaaaatc gctcgggtga tgattgagca agacgagcgg
     2401 gatttccgga tccagcgatt gctcaagcga ttgaaatgaa gcggcggtcg cttgcatgcg
     2461 ttcttgcccg cgcttgtcct gtggatattc aagcccggct acttgcagcc tgcctcttcc
     2521 gcgcgaaagc acaacatgcg aatcgcgcag aacagtgcac ccgatgcttg tcaccttttg
     2581 tgtccattct tcaatgtcgt cgtaccagtc gtgatttcct ttttacccct cgacatcctg
     2641 gtctttcgac atttccctat cctcggcgca atcgagcgtg cttcgatgcg cttacaacta
     2701 cttgcgcgag gcattcccga cagttttttt gccacagggc gaaccaaaga atgataaaga
     2761 aaaccatctt gccaatcgtg cttgcatctg cctggatctc gatttctgaa ttcgtcagga
     2821 acactttcct gctgcattca tactggttgg atcattacaa aaaccttgga atcaccttcc
     2881 ccgaacaacc aatcaacgga gcaatctggg gactatggtc actggttttt gccattgtca
     2941 ttttcatcct tgcgaaaaaa ttcaccctcg tccatacaac gattctatcc tggatcattg
     3001 ggtttggtct gatgtggctg gtgatcggca atctgggagt tctaccgact ggcattctga
     3061 ttcttgcttt cccgctgagt gtactcgagt ccttcctggc cagcctgatc ataaagaagt
     3121 tgtccgcgga ttcctgaaaa tgcccgcaca ttcgtatcag cgcgggcatt ctaaattaaa
     3181 tctcaggctg cttttgtttc gcagagcttg agaaattctt ccgtgctctc aatcgttttg
     3241 ccacgaaggt ttgcattcaa gatgcgaaca tctttcaggt tcacgttctt gatcacagtg
     3301 tcatccaggt tgcaatcact gaaaagaacc ttctccatgg aaacaatttc aaaggtaaca
     3361 tgcttgaatc cgctctttct ccatttctct ccgccggaga aaagtacgtc tgacattttg
     3421 catttcaaaa acgacgtgcc tgtcattttc aacatgcgaa tcagcacctg catcccttcc
     3481 gtttcctcaa atcgcacttg agaaacttgc gaggccagca cttcaaaatc ggccatttgt
     3541 gatttcttaa gcgtcaggct tgaaatctga gacttgtcaa aaacaccact ccaggaggaa
     3601 ttctctgcaa agctcaaatc tttcagagca gacgcaacca tccttgcctg tgtgatttca
     3661 gaatcctgaa tacggatttt tttcaagctg gagctgctga tctccaggct cttcgcattg
     3721 gccttgtcga gctccgtatt ctccatagaa gtagcagtca ggcttgttcc gctgaatttt
     3781 gaagatttca ataccagatc ctgaatactg ctggaatgga tttcgtttcc tgagaactcc
     3841 gcatcttcaa gatgcaagcc tccaagagaa gacatccgaa ttgaatttcc ttcgaataca
     3901 aaagattttc ccgcgggcgc ttgaacccga gacatggaaa aatcattctc tttgagagag
     3961 cttgcataga gtccgccgac ggaactggat agcgctccaa catctgcccc aatatttctt
     4021 agcatcgatc caagggaacc atcgttcttt tctgaacgtg tttcctttga ctgtatctca
     4081 gtcatgagtc tagcagcctg atcatctgta aggcggccgg ctctgaccat ttcaaaaatt
     4141 tgtttaactt cctgtttcat tctggttact cctttttcgg gggcagagct ttgatctctt
     4201 tcatggcctg ttcgaaattg attttgcctt cagacaggcg agacaggatt tcttctgttg
     4261 gagaattcac actcttcgcg ggcgcgtcat ccgacagtcg atccttgagc gcactgaggc
     4321 gcgcacgaac ggtcggataa ctcaggccca gggcggcttg catttcacga acacgccctt
     4381 cgctttttac gaagacgcgc aagaagtgta aatcctccgg actgagagtg gcgaactcat
     4441 tcaaaacaaa tgggccctcc actttgatgc cacaaacgtc acaaccaagg atccgcggcc
     4501 gcagaggccc ccggcaggac gggcaggcaa gactcgaatc gatcccttcg ctcattttac
     4561 cctcaactta agactcaatt tcggcgcaat attttaatat gtcaaatata tttttaatat
     4621 tttaaaacaa gacttgaatg actgaataga gtgttccttt ttttgcggaa atgtgacata
     4681 atccggtcta tgcccgcctc ccgggccggg tgggcagtct ggtgaccacg acgcggaagg
     4741 aagagcgttt cgcgcccgag gttttgacaa actcagaatt ttgttcagaa tcaagacatg
     4801 atgccgcaca tggggagatt gatttggact ctttcgctct ggatagtttg cgcgagcgcc
     4861 ggtacatgcg gccagtatcc tacttccatg ccatggctcg gccttctcgt cgctaaaaac
     4921 aattcgtctg cgagtacatc gccgcctatc gttgccacac cacagtggaa gatatttgtt
     4981 acggcgggcg tttcctctcc ttacatagga gtctccggat tcgacgcaga ttgcatgggg
     5041 gatacgaaca aacctgcaga cagttcggtt tacaaggcga tgatcgtcga aggcaccacg
     5101 cgccgtgcct gctcaacgcc gatgtgcggc ggcggcgctg cggagggaat tgactgggtt
     5161 cttaagcctt cgcaccgcta tgtgcgcgca tccgattccg cggatttgtt cactgcagga
     5221 tctgcggctg tcttttcgtt cggcacctgg agcaatcctc cggatgcggc cgccctctcc
     5281 ttctggacgg gtctgacggc ttcctggacg aattcaccat ccaattgctt gagctgggcg
     5341 aacaacctgg gtggtacgtt cggcgccacg ggcaattcaa gttctacgaa ttcttccgtg
     5401 atttccgcgg cggccctgga ctgcaccgga agctatcatc tgctctgcgt ggaacaataa
     5461 gccacctcct ttttattctg ccctttccat cacaatgatc cgcccggctt caagtgcaac
     5521 atacacaccg cgccaccacc ctttttttac cgcaaaattc gaccgggatt cagagattgg
     5581 tcgcacgtcg acttttacca atcgaatcaa atggaaacac ttccgaacta cggcctggca
     5641 aacacaatca caggctttgc cacactgttt tctggtcttt tgccgctctg catctgctat
     5701 ctcgtagagc gtcatcccgg acgatggatg ttcgtctact ggctgattgt catcaccgga
     5761 attttcacga ttacactgca tggcttcgga gaaacaaacc ccatgattct tgaaagatgg
     5821 gtgtggtctt ttctggacac aggctcaaac atcgttgttg tatgggggat cgttcttgct
     5881 gtgctcgagg atggttacag tgaacggatc agaattcggg cacggcctgc ggcgactgtg
     5941 ttgatgctcg tcgggattgt gtggcatttc tttgatcgaa tgacgtcagg cggctacctc
     6001 attagttttg gcaattgggg cgggtttcgc ccggggcaaa cctggttgat cttgttctct
     6061 ttcgcggcaa ctgcactttt ctatgcgaag agagaaacca ttcctcagaa agcgcgaccc
     6121 ctgttgagtc tgaccactgt gatctttgtt gcgggccttt tgatggccac agcccgcaac
     6181 gatacgattg ttttcccctt cctgagcctg catgcactgt ggcacttgac cgcagcattc
     6241 ggatttatct ccctctgggc gttcaatgac gttcgctttc gcgcgacccc tcggaataga
     6301 gacgttagcc ggcattcgaa ccaggaccta cagtgacatg tctcgtctgc aaattctcta
     6361 gtttctgacg gaccggtcct tcgtttgcgc gaaactccgt ttcgtatgca ggatcgattg
     6421 taccaaacca gcggtcccac cagtaaaacc acagaccgta attcactcga aacttcttgt
     6481 gatgttgatt gtgcagagtc gcggtgatga cccagcgtcc aattgggtga ctggcaaacg
     6541 ctcgcggtaa gaattcgtat ccaaggtgcg catagacatt aagcaacaac atgatcgaag
     6601 ggtatgtgtg ggtaatgcta atgtgcaagg gcaagatcat aatcattgga ataaagaatg
     6661 catactcaac cactgcctcg agcggatgaa acgcaaaagc ggccagtggc gaaggattcg
     6721 tcgacctgtg atgaacagca tgcacatagc gaaagatagg cttccagtgc ataaagcgat
     6781 gagcccaata aaagtaggca tcgtgtgcca ggataaaaag aatccaggag agtaagtagt
     6841 acccccagcc gtaatcagag atgctgttgt atcgtctggt tagacccgct cgcatcagta
     6901 gaactacaag gatcgccatg agcgcaaaca cgaacaaagt gctgacggac cagcctactt
     6961 ctttccacag tcgatccctg gatggatttg ttttctgaat ggcacgtgat ccgaattttt
     7021 gcctcgcaat aaaatacacg aatgcaaacg caagcgcggc gaatactgca tagcgcacca
     7081 accagaaagt gaaatcaaac agaaagacgg ccttcgccat ttccagggga gtgagatttg
     7141 ttgagtccaa agtaagtcct cctttcattt ggatggactc agtatcgcac gcttttgcgt
     7201 gcgcgtcgcc ctcgcgtata cgcaaggagc tcctggatta tacgcacgga ccaggcatga
     7261 tgggcttatg cttgcctggt taaattcttc ctataatcag atggagattg tcccgttgcc
     7321 ttgacgaatt ctgcattgaa agacgattta gatccgaacc caaccgcccc catgatcgaa
     7381 agcaccgcgc gatcgggctc ctggaccagg agttcgcatg cagcgcgaac cctgtaccca
     7441 tttacgtagt tcttgaatgt cagaccaatc ttatcgttta ggatttcaga aagcttgtgg
     7501 ggagtcagat ccaggatagc ggccagacga gcgagatcca gatcttcatc tgagtagagt
     7561 gcttcgattt ccataagttc cgttaaacga cgaatcaaag catctgaatc caggcccccg
     7621 agtctcgaac gttcgtatct cgctctcgag gcttccttct tcattcgatc cagatactga
     7681 ggaaaacgct gcgctcccca gaacatgagc acgggcaaga atccaagtcc caagaatccc
     7741 agggtggttg ccatttgact cctctggatc tgattccaga cgagcagtga aacgaacaca
     7801 gctcccagga aaacaaacac tgctgttaat ttgtactccg gaggcaactg tccattcctg
     7861 ttttgaagga gcgaaaagaa atcgcgcaac accagtgcca gaaagatcaa agtataaact
     7921 gaaatgccaa caaagatgag atccagatac cattgaaacg tgcaccggcc gtgagccatc
     7981 ctgtccagtt ccgcgtcgga aagaaagtag agcggcaaaa gtgcaatttg cacgatcgca
     8041 aagggaacca aatgcaatag ttgatgcttt ttctgaacag ggcgaaaccc gggctcaatg
     8101 acttgatgga aatagaaata cgcaagtgga ccgaagaaca cataaaaggg tacgtgagga
     8161 aaaagagcgc gcggataaat gaaaaacaag cccaatcgtt cgaatgcaat ggaaagttgc
     8221 ggaaaagaga ggagaatgaa caagacaccg agaattcgat ccgattgagt cgacggatgt
     8281 ttcagaaggc gcgccatccc gattacgatc gcgagggtcg cgccaatcag agtgccggcg
     8341 ataaagaaat ctatactggt ccagcgaagc atttggaatt ccggcgatcg aggcttgagc
     8401 gctctgtcgc tcaaatgttt actctggtaa gaaatcctcg cgtgcatagt aggattctca
     8461 gctatgtaaa agaaaaaggc tattttgaaa gccttctagt tccctgtgat caagcattgg
     8521 ccgcctaacg ttaggccaaa ctacgaggca ggaggtagcg cgggagacgc atggaatctt
     8581 tgttgcgacc tgccgcgcac cactgagctt cgctctatga acccggcgca ggaaattctc
     8641 aagctgcttg agaagcaggc cggagagatc ccggagtcag tgcgaaagtc ctttatcgca
     8701 ggcgcgcgcg agcttgtggt tccatccggc gcgcaaattg cgtttgaaaa cgaagcctgc
     8761 gcgcggctgc cttttgtgct cagcggaggg cctcgggtct acaaggcggc acccgacggc
     8821 aggtccatca ctctatacga catcgatccc ggggaaacct gcgtccttac gatgagctgc
     8881 attctgggtg gagaaccatt ccctgccggc gctgaggctc tcggagaaac gcgagtcctg
     8941 gtagtctcgt ccgaagactg gctgcgctgg gtggagagct ctccctctct gcgtcgttat
     9001 tcgcaccaga ttgcggcacg acgattgagc gccgtcatcg aaacacttag cgaggtggct
     9061 tttcatcggg tcaaccagcg cgtggcccgc ttgattgttc gccgctccac aggcagcggt
     9121 atgcaggcaa cacatcagca gatcgcagat gagctcggca cagcccgcga agtggtcagt
     9181 cgcatcctga aggaatttga acgctatgga tggatccgca tgtcccggca tgaaattgcc
     9241 ctgctggaca aatcggccct ggtgaagctt gcagaagatg ccgacgctgt gtgatctatg
     9301 tcacagacag gtctagcatt tcgtgctata ttcctacatg ttcaacaaga ttgaacggag
     9361 gaatatatga aaaagaatat gggaaccata gaccgcgctt tgcgtgttgt ggctgcactg
     9421 gcagtaggcg tgctctggta tctgggcgtg atttctggca cagttgccat cgtgctcggc
     9481 gtcctggcag ttgtattcgt gcttacgagc ctgatcagct tctgccctct ctaccttccc
     9541 tttggcctct ccactcgcgg caaagaataa ttgcgagagc atgactgcca cccgcatcgc
     9601 cggcgtggca ttcatgccgc cgcgtggtat accgttaggc gatttatatt ggaccacgca
     9661 ctcttcacta cgcgcatgca tgaattcatt ttcgcaagat ctgtccgtaa ctcgtaccgc
     9721 tcacaacggg aacatctcct ttaaagaggt tacattccat ttcgattttc tttcgcagac
     9781 gcttatcaat cactgcagtc agggtg
//
LOCUS       DAORFF010000060         9800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3211_length_9800_cov_3.815700, whole genome shotgun sequence.
ACCESSION   DAORFF010000060 DAORFF010000000
VERSION     DAORFF010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9800
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3211_length_9800_cov_3.815700"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..407)
                     /locus_tag="PKK76_03955"
     CDS             complement(51..407)
                     /locus_tag="PKK76_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67648.1"
                     /translation="MKVVFSFLRAMRSPALAAALLMIATCSSSTSANTELAEAPKLTE
                     IEGTIQVKGSASAPITSIDLPGGLSYTLQGKLEKMVRGMYRGKNLKIAGKIITDAKPG
                     KPGVFEVKEIVVILTQ"
     gene            complement(404..730)
                     /locus_tag="PKK76_03960"
     CDS             complement(404..730)
                     /locus_tag="PKK76_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNL67649.1"
                     /translation="MKNVLFIAVMALLFHCKQSTENLTPEEKFKAEGRSLFVANCIAC
                     HSANPDQDGSVGPSLRASSFDLLKEKLINGKYPDGYKGKRPISGSMPKFAFTDEQIRS
                     IEAFLK"
     gene            complement(782..1489)
                     /locus_tag="PKK76_03965"
     CDS             complement(782..1489)
                     /locus_tag="PKK76_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004764034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HNL67650.1"
                     /translation="MEFAILSKQGLYFLLKWSHFIFGIAWIGMLWYFNFVQGPFFAET
                     DANTKTAATQKLVPRALWYFRWGAAFTLLSGLLLIGLNFHLSGATAGEWATGSWLAKI
                     YPGIIMGIVMAANVWFVIWPRQKKVIAAANGEKVENLPVLARRAFLASRTNTMFSIAL
                     LFFMGAGGSKLPGAFNGANVGAWYGVIALIIGLLEINALVATNGITTKPIEKPVGVIH
                     MGLVLAIVLYVILVAML"
     gene            1651..1992
                     /locus_tag="PKK76_03970"
     CDS             1651..1992
                     /locus_tag="PKK76_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67651.1"
                     /translation="MNAISRKDFLKKGAVAGAVGAAAVALAYCKKKGGEEASAPSAGG
                     CSDVSGLTQAEKDTRAQLKYMDKSDQEGKACNGCALFVPAAAGAACASCNLVKGPISP
                     EGYCTSWVKKG"
     gene            2078..3937
                     /locus_tag="PKK76_03975"
     CDS             2078..3937
                     /locus_tag="PKK76_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HNL67652.1"
                     /translation="MTLADLLQKFPWPERYRSLGRPLEWFWNFRVAIPRAKLWPLIMD
                     TSRFNRLMELPAMNFEEKDGILYGSSRNAGFHLEWKEIPWSWTSEHTLTNAREYSKGF
                     AHVVRAVYVLEEESAEAVNLTVYFGWIPRNFFGRVLLFFAGSSIKRSYAKALAAIEAS
                     VNAPGATNPFVPPAPTLSPSSLARLNEIRTTLRSSGVDEAISARLVDYVQNGDDLDLY
                     RIRVGALARIWNVDRRALLRGCLHATRAGLLTMSYDIICPHCRGVRQEVSTLGDVPER
                     GNCEVCEIDFTNESEGIVEITFHVHPSIRSVPRVFFCSAEPAQKRHIKLQQYLEPGSK
                     LDAELPLATGRYRLRVKGFSKPGLLMVGPDPEGFVEWTAEDREFRAESGQMSRFSIKN
                     PTEKGQTFTLETTEKDPDALLPAALFNLQEFHDLFSAESLAADLQLEIGEQTILFTDM
                     VGSTSFYASSGDSKAFVAVKKHFEEVYREVRDANGAVIKTIGDAVMASFADPVDAMHA
                     AVSLQNKFGPSSPSPIRLRVSIHTGQCIAVNLNSGIDYFGTTVNVAAKIQNVAGAGEI
                     ACTAQFLAKPGVRAFLSQRGLSGESMPYRIPGLSDTWSLERFRITAPEVARNA"
     gene            3968..5095
                     /locus_tag="PKK76_03980"
     CDS             3968..5095
                     /locus_tag="PKK76_03980"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL67653.1"
                     /translation="MRWQYCARCSALCIAVFSTVCSFPAGKTSGGLQSLLLADALFLS
                     GRLPRFLFATTNTGNVISRYRVEPLSGYLTFLGTTPSGGTSPRGASIDPTGRYLMVVN
                     NGSTNVSSYSINRFDGQLTLVGNTASGISPISAYFLPSGSFAYATDAGGAPYVMGYTM
                     NSSTGVLTNFAVGATGLNVGRGAVSDSASRFLYVGDFSGNFVRSFTINANGTLTSLNS
                     VATGVNPHSLAMHPGGQYLYASLNSGSLVAFRVASDGTISMTATVAVSGTPQTIAIDP
                     LGRYVYLVNATGGVLYVFSIADPTSPVLLYTFVMGPQANAVRVDPSGRFVYILCGTGS
                     NQIFALYLDPFWGFSFLTALSTGSNAGAMEGIAIASYSISE"
     gene            5188..7122
                     /gene="flgK"
                     /locus_tag="PKK76_03985"
     CDS             5188..7122
                     /gene="flgK"
                     /locus_tag="PKK76_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNL67654.1"
                     /translation="MGSTFGGLEIGRKGLVAHQTALNTTGHNISNADNNGYSRQRVTM
                     EAADPLYEPSLNRASGPGQIGQGGSVSRIERVRDAFYDDQIIDATNRQNYWDASTNYL
                     GQMETIFAEPSDNTLRSLTEKFWASWQDLANYPSDAAHRSVVAERGNGLITRIHDIHD
                     KLGQLRIRANNEIVADVDILNSLAGEVRDLNEKILKLEALGDEPNDLKDKRDAVIEKM
                     SGIANIKVGRGDRDEVFVFIGEQTMVQGSILRKIKAEVDPANEGYAKVVWEHNSRDVI
                     LGGGHLSGLLEMRDHAIVERMDQNSLFAVNLADIVNEIHRDGFGINGTTNKDFFQIRN
                     LSPGSDGNYQLQNARANVDLNGDGTAELTAIFRTTGANSVDPSKKIGVDGTITFFKND
                     AQNTPVRIDYRRDQTLNEIIKKINDADGGVVAYMNHDNQLSIKATLASDDRRTNFMIR
                     HLEDSGELLVGYAGVLASSGEAGAFDFRRVNEIQKLRAPLQDIAFTPSFYPAGQISLS
                     SDIVRDPSNIAAARGRDLGGTGDYNTPNGAADGSNALLIAAGLKQDKKMFGHEETVDN
                     FYNALIAKLGTQARTAEDAAMRQKENLTQLGNFRQSVMGVNMDEEMSNMVQFQQSYQA
                     SARVINTMNEMVETLLKLGV"
     gene            7127..8392
                     /locus_tag="PKK76_03990"
     CDS             7127..8392
                     /locus_tag="PKK76_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HNL67655.1"
                     /translation="MRITDLMKNNTLVSNVNRHQYEMDKVQNQLATGQRIRQPSDDPG
                     AATNQMFFRTRVEELDQFQANIEDGHSRLMLMDGELDRVGAIFQRVRVLAVQASNGIY
                     QGDNAFELKNAMAKEIDQHLRALIDIANGRDAVGRPMFGGDIVERMPFEPVVSNIRGG
                     KGIELDNMIVGVEYRGNIGQQLREVERSQYIDVNLPGNKVFWGTNMTVTGSVDNSGYR
                     ATTDQVFKIDGQTIRVAAGDTIDDIIDKINNASLEVKASKIGQDFASLHTTNPHQVWL
                     EDIEGGTVLRDIGLINPDKANPPNNFAETARVSGLSVFDVLIKLRNDMIAGDQLEISG
                     RDLGNLDEGLNNILRYRAEVGARQNRMDEHEKRVVWDQTYMTELLSKSEGVDMPEAIV
                     NLKYLEAVHQYALNIGARLMKPTLMDFMR"
     gene            8403..8855
                     /gene="fliW"
                     /locus_tag="PKK76_03995"
     CDS             8403..8855
                     /gene="fliW"
                     /locus_tag="PKK76_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNL67656.1"
                     /translation="MTKTGRTLETKALGKITLDPEQLLSFPEGLYGFRDFHEFALVEE
                     SAENPFKWLQSTSDPALAFIVIQPELILSGYRPEVAREDLQSIGLSGVDESLVFLIVT
                     IPENDPESMTANLQGPVLINKTTKQGRQAISMNEKHVVRLPVLSQVDA"
     gene            8855..9091
                     /gene="csrA"
                     /locus_tag="PKK76_04000"
     CDS             8855..9091
                     /gene="csrA"
                     /locus_tag="PKK76_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNL67657.1"
                     /translation="MLVLARKINESIMIGDDIEIVVVDIKGDQIKLGIRAPRSVSVHR
                     AEIFKEIQEQNKKAVESAPQSLGKLKNLLDKKKQ"
     gene            9114..9467
                     /locus_tag="PKK76_04005"
     CDS             9114..9467
                     /locus_tag="PKK76_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67658.1"
                     /translation="MRAVLFIFLSCFSATLGAEPDGSKMDAHTSINYRITNRKYVSHI
                     LLERSGGINYVTLLTTHPMQWAFHSESHALKYACGKIPDCMDLMSRLDECLNAGKNLN
                     LHLDGERIIEIQVYE"
     gene            complement(9472..>9800)
                     /locus_tag="PKK76_04010"
     CDS             complement(9472..>9800)
                     /locus_tag="PKK76_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67659.1"
                     /translation="TPFVPIASGKAENSTQWKEQPLRQLSGAVGIYFIRPDGNMALKL
                     FDGVRPAGSETALIYVPYVSMSREKNRYYLLPLYPFTMILDVLTIALQIAIWDEEIDT
                     WTRCLK"
BASE COUNT         2464 a         2382 c         2544 g         2410 t
ORIGIN      
        1 tgggaacata tgtatgattt accttcggtc tgaatgcgtt cgggattgag tcactgcgtg
       61 aggatcacaa caatctcttt tacttcaaag actccaggtt ttcctggttt ggcgtctgta
      121 ataatctttc ctgcaatctt caggttcttg ccgcgataca tgccccgaac cattttttcc
      181 agttttccct gcagagtgta ggacaaacca ccgggcaaat caatggaagt gatgggagcg
      241 ctggcagaac ccttcacctg aatggttcct tctatttcag tgagcttagg agcttccgca
      301 agttccgtgt ttgcagatgt cgaagaagag catgtggcga tcattagaag tgcagccgcg
      361 agagccgggc ttcgcatcgc gcgaagaaaa gaaaagacca ctttcatttg agaaatgcct
      421 cgatgctgcg gatttgttca tccgtgaatg cgaatttggg catagaacca ctgatcgggc
      481 gtttgccttt gtatccgtcc ggatacttac cgttgattag cttttctttg agcaagtcaa
      541 aactcgacgc gcgaagcgac gggccgacgc ttccgtcttg atccggattt gcgctgtggc
      601 atgcgatgca gttggcaaca aatagactgc ggccttctgc tttgaatttt tcttctggcg
      661 tgaggttttc agtgctctgc ttgcagtgaa acaagagtgc catcacggcg atgaacaaca
      721 cattcttcat aaatttggat tccagtttgt gagatggggg cgtttgccgc cccctgaagt
      781 cttacagcat tgctacgaga attacgtaga gaacaattgc aagaacaagt cccatgtgga
      841 ttactcccac aggtttttca attggttttg tggtgatacc gttggttgca acgagagcat
      901 tgatttcaag caggccaatg atgagtgcga tcactccata ccatgcgcca acgtttgctc
      961 cgttgaatgc accaggaagt ttcgatccgc cggctcccat gaagaagagc agtgcaatcg
     1021 agaacatagt gttggttctg gacgcgagaa aagctctgcg agcgagcaca ggaagatttt
     1081 ctactttctc accattcgca gcagcgatga cttttttctg gcgaggccag attacaaacc
     1141 acacgttggc tgccatgacg atccccatga tgatgcctgg ataaatcttt gcaagccagc
     1201 tacctgtggc ccattcgcca gcagtcgcgc cgctgagatg gaagttcagg ccgatcaaaa
     1261 gaaggccgct gagaagagtg aaggccgcgc cccaacgaaa gtaccacagc gctcttggta
     1321 ccagtttctg ggtagcagct gtcttggtgt ttgcgtccgt ttcagcaaag aaaggaccct
     1381 gtacgaaatt gaaataccac agcatgccaa tccaggcgat gccaaagata aaatggctcc
     1441 atttgagcag gaaatagagt ccctgtttgc tcagaattgc gaattccatt gattcctccg
     1501 agattttctg ccagtgactg gcattctcat cacagttaaa ggaaaatttt gagaccgagt
     1561 atcaaatcgc gccagattcg gagcagtcag gcaaaaaaga ttcgttaggc cttccaggga
     1621 agccgttctg gtagcttaaa cggaggatcc atgaacgcga tctctagaaa agacttttta
     1681 aagaaaggcg cagttgccgg cgctgtgggt gctgcggctg ttgcactggc ttattgcaag
     1741 aaaaaaggcg gcgaagaagc atctgctcca tcagcgggcg gttgctcaga cgtatccggc
     1801 ctcacacagg cagagaaaga cacaagagca cagttgaagt acatggacaa gagcgaccag
     1861 gaaggcaaag cctgcaacgg ttgtgcattg ttcgttccag cagcagctgg cgcagcgtgt
     1921 gcgtcctgca atctggtaaa aggtccaatc agcccggaag gctactgcac ttcctgggtg
     1981 aaaaagggct gagtttctta cggctgcgca ttcgtatgcg cagcctctcc ttccaaaaat
     2041 attcgccatt gcgtcggcat ttgatttttt cgagtctatg actctggccg acttacttca
     2101 aaaatttccc tggcctgaac gatatcgctc tctgggtcgt cccctcgaat ggttctggaa
     2161 ctttcgcgtt gccattccgc gcgcaaagct atggccactg atcatggata caagccgctt
     2221 caatcgtttg atggagcttc cagccatgaa ctttgaagag aaggatggca ttctctatgg
     2281 atcttctcgc aacgcaggct tccatctcga atggaaagag attccctggt cctggacttc
     2341 tgaacacact ctcacaaacg ctcgcgaata ttcaaaaggt tttgctcatg tagtccgcgc
     2401 cgtgtacgtt ctggaggaag aatccgcgga agctgtgaat ttaacagtgt actttggctg
     2461 gattccaaga aacttctttg gaagagtact tcttttcttt gcagggtcat cgattaagag
     2521 aagttatgcg aaggcgctcg ccgctattga ggcttctgtc aatgctccag gcgccacaaa
     2581 tccctttgtg ccacccgcgc caactctatc gccttcttct ttggccagat tgaatgaaat
     2641 tcgcaccacg cttcgatcca gcggagtgga tgaagccatc tctgccagat tggttgatta
     2701 cgttcagaat ggagacgacc ttgatctcta tagaattcga gtcggagctc tggcgcgcat
     2761 ctggaatgtg gatcgacgcg cgcttcttcg aggctgtctt catgcaacga gggccgggct
     2821 attgaccatg agctatgaca tcatctgccc acattgcaga ggagttagac aagaagtatc
     2881 cacgttaggc gacgttccag agcgcggaaa ttgtgaagtg tgcgagatcg actttacgaa
     2941 tgagtctgaa ggtatagtgg agatcacgtt tcatgttcat ccttctattc gcagtgtccc
     3001 acgtgtattt ttttgcagtg cagagccggc gcagaaacgt catatcaaat tgcagcagta
     3061 tctggaacca ggatccaaac tggacgctga attgcctctg gccactggtc gttatcgcct
     3121 tcgcgtgaaa ggtttttcaa agcccggtct tttgatggtg gggccggatc cggaaggatt
     3181 tgtggaatgg accgcagaag acagggaatt tcgcgcggaa tcgggtcaga tgtctcgctt
     3241 ttctatcaag aatccaacag aaaaaggaca aacgttcact ctggaaacta cggaaaaaga
     3301 tccggatgct cttttgcctg cagccctttt caacttacag gagtttcacg atctgttttc
     3361 cgcagaatcg ttggctgcgg acctgcagct tgagatagga gagcagacga ttctgtttac
     3421 ggacatggtt ggttctacca gcttttacgc ttccagtgga gattcaaaag cgtttgttgc
     3481 ggtgaagaag cattttgaag aagtctacag ggaagtgaga gacgcaaacg gtgctgtgat
     3541 caagacgata ggcgacgctg tgatggcttc tttcgcagac ccggtcgacg caatgcatgc
     3601 ggctgtttct cttcaaaata aatttggtcc ttcgtcgcca tctccaattc ggcttcgcgt
     3661 ttcgattcac actggccagt gcatcgctgt gaatttgaac agcggcatag actattttgg
     3721 aaccactgtg aatgtagcgg ccaaaatcca gaatgtagcc ggtgccgggg aaatcgcatg
     3781 tactgcccag tttctcgcaa agccaggagt gcgcgctttc ctttcccagc gtggcctgtc
     3841 cggcgaatct atgccctacc gcattccagg tctttcggat acgtggtctc tggagcgatt
     3901 ccgcataact gctccagaag ttgcgcggaa cgcttgacat gaattgagaa cagcgctatc
     3961 gaagggtatg cgctggcaat actgtgcgcg ttgcagtgcc ctttgtatcg cggttttctc
     4021 tactgtttgc tcatttccgg ctggaaagac atctggaggg ctccaatcgt tgttgcttgc
     4081 ggatgcgctc ttcctgtccg gaagattgcc gcgctttctg ttcgcgacga ctaacaccgg
     4141 gaacgtgatc tcaagatata gagtggagcc gctctccggt taccttacat ttctgggaac
     4201 aaccccttcc ggaggcactt cacccagggg cgcgtccatt gatccgacag gaagatactt
     4261 aatggttgta aacaatggat ccaccaatgt atcatcctat tccatcaatc gttttgatgg
     4321 tcaactgacg ttagtcggca ataccgcatc cgggatcagt ccaatcagtg cctactttct
     4381 tccgtcgggc tcttttgctt atgcgacaga tgccggcggt gccccctacg tgatgggtta
     4441 cacaatgaat tcttccaccg gtgtcctgac taattttgca gtcggagcaa ccggtctcaa
     4501 cgtcggtcgt ggcgcggttt ctgattctgc cagtcgcttc ctgtacgtgg gcgatttttc
     4561 tggcaatttt gttcgatcgt tcacaatcaa tgcgaatgga actctgacat ctttgaacag
     4621 tgtggcgaca ggtgtaaatc ctcacagtct ggcaatgcat ccgggcggcc agtacctgta
     4681 tgcgtccttg aattctggtt ctctggttgc gtttcgagtg gccagcgatg gtacaatatc
     4741 tatgaccgct accgtagctg tttccggaac cccgcagaca attgccatcg atccactcgg
     4801 aagatacgtc taccttgtaa acgcgacagg cggtgtgctt tatgtattct ccatcgcaga
     4861 tcccacatcg cctgttttat tatacacatt cgttatgggc ccgcaggcca acgcggtcag
     4921 ggtcgacccg tcgggaagat tcgtctacat tctgtgcggc accggttcaa atcagatttt
     4981 tgctttgtat ctggatccat tctggggttt cagcttcctt accgcgctca gtaccgggag
     5041 caatgcaggc gcaatggaag gcattgcgat tgcctcttac tcaatttcag agtagaattc
     5101 acagggtttt ttaatcatct tttgcgctga atgtccgaca cgttcgatag acacgaatcg
     5161 ctcgtgccat gcacggcggg aggacatatg ggttctacat tcggcggcct cgaaatcgga
     5221 aggaagggcc tggttgcgca ccagactgcc ctgaacacga ctggccacaa catatcgaat
     5281 gccgacaaca acggttattc ccgccagcgc gtcaccatgg aagcggcaga tccactgtat
     5341 gagccttctt tgaatcgtgc ttccggtccc gggcaaattg gccagggcgg aagtgtttcg
     5401 cgcattgaaa gagtgcgcga tgcattctac gacgatcaaa tcatagacgc aaccaatcgt
     5461 cagaattact gggatgcttc taccaactac ctgggacaaa tggagacgat ctttgctgag
     5521 ccatccgaca acactcttcg ctccttgact gagaaattct gggccagctg gcaggatctt
     5581 gcaaactatc cgtccgacgc agctcacagg tctgtggtcg cagaaagagg aaatggcctc
     5641 atcactcgca ttcacgacat tcatgacaaa ctggggcaac ttcgcattcg cgcaaacaac
     5701 gagatcgtag cggacgtgga catcctcaat tcgcttgccg gtgaggtgcg tgacctcaat
     5761 gaaaaaattc tgaagctcga agccctggga gatgagccaa acgatctgaa agacaagcgc
     5821 gacgcagtca tagagaagat gtccggaatt gccaacatta aagtagggcg aggagaccgt
     5881 gacgaggtat tcgtattcat tggcgaacag acaatggttc agggctccat cctgcgcaaa
     5941 atcaaagcgg aagtagatcc agcgaacgaa ggttatgcga aggttgtttg ggagcacaat
     6001 agcagagatg tgattcttgg cggcggacac ctttccggcc ttcttgaaat gcgcgaccat
     6061 gcaattgtag agcgcatgga tcagaacagt ctttttgcag tcaatctggc ggatatcgtc
     6121 aacgaaatcc acagagatgg ttttggaatc aacggaacca caaacaaaga tttctttcag
     6181 atcaggaacc tgagccccgg atcagacgga aactaccagc tccagaatgc acgcgcgaat
     6241 gtggatctca atggagatgg aacggctgaa ctcactgcga tctttcgcac aacaggcgcg
     6301 aactccgtgg atccaagcaa gaagatcggc gtggatggaa ccatcacatt cttcaagaat
     6361 gatgcgcaga acactccggt tcgcatcgac tatcgtcgtg atcagactct caatgagatc
     6421 ataaagaaaa taaacgatgc ggacggtggt gttgtggcat acatgaacca cgacaatcag
     6481 ctttccatca aggcaactct cgcaagcgat gatagaagaa ccaatttcat gattcgccac
     6541 ctcgaggatt ccggagaact tctcgttgga tacgccggag tgctcgcgtc ttcaggggaa
     6601 gcgggtgcat ttgattttag aagagtcaat gagatccaga aactgcgtgc gccgcttcag
     6661 gacattgcgt ttactccaag tttctatcct gcgggacaga tttctctgtc ttctgacatt
     6721 gttcgtgatc catccaacat tgctgccgct cgcggacgtg accttggtgg aacaggggat
     6781 tacaatactc cgaatggagc cgcagacggt tccaatgcgc tgttgattgc cgcgggtctc
     6841 aagcaggaca agaagatgtt cgggcatgag gagacagttg ataattttta caatgcgttg
     6901 attgcgaagc tcggaacaca ggctcgcacc gcggaagacg cagccatgcg tcagaaagag
     6961 aacctcactc aactgggcaa tttcagacag agcgtgatgg gtgtgaacat ggatgaagaa
     7021 atgtcaaaca tggtgcaatt ccagcagagc taccaggctt ctgcgcgcgt gatcaacact
     7081 atgaacgaaa tggtagaaac tctactgaag ctgggcgtgt aatatgatgc gaataacgga
     7141 cctgatgaaa aacaatacgc tggtttccaa tgtgaaccgg catcaatatg aaatggacaa
     7201 agtccagaat caactggcca cgggccagcg cattcggcaa ccaagcgatg atcctggagc
     7261 cgccaccaat cagatgttct ttcgcactcg ggtggaagag ctcgatcagt tccaggccaa
     7321 catcgaggac ggtcattctc gtctgatgct catggacggc gaactcgatc gtgtgggagc
     7381 gatttttcag cgtgttcgag tgctcgcggt gcaggcttcc aacgggatct atcaaggcga
     7441 caatgctttt gaattgaaga atgcaatggc caaagagatc gaccagcatc ttagagcgct
     7501 cattgacatt gccaatggcc gcgatgctgt ggggcgtccc atgttcggcg gcgacattgt
     7561 agagcgcatg ccgtttgaac cagtggtctc caacattcgc ggaggcaagg gcatcgagtt
     7621 agacaacatg atcgtgggag ttgagtaccg cggaaacatt ggccagcaac ttcgtgaagt
     7681 ggaacgctct cagtacattg atgtaaattt gcccggaaac aaagttttct ggggaacgaa
     7741 catgactgtg accggctccg ttgacaattc cggctatcgg gccaccacag atcaggtctt
     7801 taagatcgac ggacaaacga ttcgtgtcgc tgccggagat acgatagacg acatcatcga
     7861 caaaatcaac aacgcaagcc tggaagtgaa agcttctaaa attggacagg actttgcatc
     7921 tcttcatact accaatcctc accaggtctg gctggaagac atagaaggcg gaactgttct
     7981 tcgcgacatt ggcctgatca atccggacaa agccaatccg cccaacaact tcgcagagac
     8041 tgcgcgtgtt tcggggttga gcgtatttga tgtattgatc aaacttcgca acgacatgat
     8101 tgcaggtgac cagctggaaa tttcagggcg tgatcttgga aacctggatg aaggactcaa
     8161 taacattctg cgttatcgcg cggaagtagg tgcgcgccag aatcgtatgg acgaacacga
     8221 aaagcgcgtt gtatgggatc agacttatat gacggaactg ttgtccaaga gcgagggcgt
     8281 ggatatgccc gaagcgattg tcaatctgaa gtatctcgaa gcggttcatc agtatgcgtt
     8341 gaacattgga gctcgcttga tgaagccgac tctgatggat ttcatgagat aaggaattta
     8401 gaatgacaaa gaccggacgc acactcgaaa ccaaagcact cgggaagatt actctggatc
     8461 ccgaacaact gttgagtttt cccgaagggc tgtacggttt tcgcgacttt catgaattcg
     8521 cgctcgtaga agaaagtgca gaaaaccctt ttaaatggct gcaatctacg tctgatccgg
     8581 cactggcatt catcgtcatt caacctgaat tgatcctgag tggctacaga ccggaagtgg
     8641 ctcgcgaaga tttgcagtcg attggactat ccggcgtaga tgaatcgctt gtgttcttga
     8701 tcgtaacgat tcctgaaaac gatccggaaa gcatgactgc caatttgcag ggtcccgttc
     8761 taataaacaa gaccaccaaa cagggccgtc aggcgatctc catgaacgaa aagcatgtgg
     8821 ttcgccttcc tgtcctttcg caggtggatg cctgatgctt gttcttgcaa gaaagataaa
     8881 cgaatccatc atgataggtg acgacattga gatcgtcgtc gtggatatca aaggggacca
     8941 gatcaagctt ggaattcgcg cgccgcgcag cgtttccgtt catcgagctg agatattcaa
     9001 ggaaatccag gagcagaaca agaaggcagt ggaaagtgcg ccgcaatcac ttggcaaact
     9061 caagaacctc ctggacaaga agaaacaata gtcgccatac ggtctgaggt ctcttgcgcg
     9121 ccgtcctttt catcttcttg tcctgtttct ccgcgacgct gggcgcagag ccagacggat
     9181 cgaagatgga cgcgcatact tccatcaact atcgcattac aaatagaaag tacgtaagcc
     9241 atatcttatt ggaaaggtct ggcggaatca actatgtaac tctcctgaca acgcatccca
     9301 tgcagtgggc atttcactct gaatcgcacg cgctgaaata tgcgtgcggc aagattcctg
     9361 attgcatgga cttgatgagt cgcctggatg aatgtttgaa cgcaggtaag aatctaaatc
     9421 ttcacctgga cggtgaacga atcattgaaa tccaggttta cgagtagcgc gtcacttcag
     9481 gcagcgcgtc caggtatcta tctcttcatc ccaaatcgca atctgcaacg ctatcgtcag
     9541 tacgtccagg atcattgtga atggatagag aggtaacagg tagtaacgat tcttttcccg
     9601 cgacatggac acatagggta cgtagatcaa tgcggtctct gaacctgctg gccgaactcc
     9661 atcaaagagc ttgagtgcca tgttaccatc tggccggata aagtagatgc ccaccgctcc
     9721 agagagttgc ctcaaaggtt gttccttcca ttgagttgag ttttctgcct ttccagatgc
     9781 aatcggcacg aaaggtgttt
//
LOCUS       DAORFF010000061         9782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3224_length_9782_cov_3.790377, whole genome shotgun sequence.
ACCESSION   DAORFF010000061 DAORFF010000000
VERSION     DAORFF010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9782
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3224_length_9782_cov_3.790377"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2688
                     /locus_tag="PKK76_04015"
     CDS             <1..2688
                     /locus_tag="PKK76_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNL67660.1"
                     /translation="DLLGLTNSQILGKTSFDPSWNVVHEDGSDFPPPDHPVPRAIREK
                     KAVQNVVMGVFRPARQDRAWLLVNAIPEISADGSVVQVLCTFTDISAIKTAETALRDN
                     ERQYRGFIELSPDGVVVHQEGVLVYANRAMARILGAHGPADLIGRRVLDLVAPEMQQE
                     IKDRIQGILSEGKIAPLREEKLVRLDGGLIDVEASGAPHTWRNKPAVLVVVRDITDRK
                     KAEARAKENELLYKSIFDAAGDGLFLADRTPRYVDVNQAGLDMLGYTKEELLRMGPAD
                     LVSKEDLEKNPLRTKDMFEQPGVVVRRKLRRKDGTILEAEINARPITPALTLGIAREM
                     GARLAAEIALRDSEEKWKLIAENVPAFILLVDRQGKILFINHLFPGWKREEIIGKQIA
                     DFMPPEEQTLTPVRLADVFERGLEIEYEQAAAGSPGEERHYASRIVPIRHEGAITAAL
                     LVATDITARKKDVARLEHQASHDSLTDLPNRQFLIESALEAIGRAEKEGRQLALLLMD
                     LDRFKEINDTLGHFTGDLLLKRIGPRMESYLKSIGAEMARLGGDEFGILLPRIDEPAE
                     AEGIAQTLHHLLREPFLLEGMNLEIDCSIGVAVYPFHGQDPISLLRCADVAMYLAKKK
                     SVGVAMYAPDQDRYSRRRLALLTELGLAIRESQLTLHYQPKYSLHDRKLVGVEALVRW
                     HHPVHGMVSPGEFVPLAETGNLIIPLTNWVFEEASRQWRVWKNAGHTVKIAVNLSVRN
                     LMDEQYPLTVDAILKKHKVERSCFELEITESAIMADPERALSVLNRLHSLGVDLSIDD
                     FGTGYSSLAYLSRLPIDALKIDLSFVRQMMESERNMVIVNSTVRLAHTLGLIVIAEGV
                     EDERTLNTLSDMKCDMAQGFFFSPPRRAEDLTEIIARDL"
     gene            complement(2697..3278)
                     /locus_tag="PKK76_04020"
     CDS             complement(2697..3278)
                     /locus_tag="PKK76_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNL67661.1"
                     /translation="MRFAYLLLVASFALVSPVASRDLQNHELEIRYGPAQTCGTCAGQ
                     HLWQPMAKADALFQGTLNVSDDWILGGYGKASVYDHIFQGASGFLLGWNLGLVEARGY
                     AGIAYAGGVTERVRRGFFVYDGQTRATYDLGVSLRYPVADRWRLTLNYAHNSNGEDIG
                     MNFTGLRRKNPGIDSISFGVSYVLGQTPALLLP"
     gene            complement(3275..4858)
                     /locus_tag="PKK76_04025"
     CDS             complement(3275..4858)
                     /locus_tag="PKK76_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNL67662.1"
                     /translation="MTVNQYDAIVIGSGIGGLAVASILAQKEKKKVLVLERHFRAGGF
                     THEFTRKHYHWDVGVHYVGDMQEGGMMRRLFDVITDSKVDWEKMNEPFEKFVYPDFTF
                     PVFGNEERFIADLIEKFPAEEAAIRKYVADAKRAASWFGRHITMKSLPPFLDKLTHLV
                     DSLGLSSALMTTKQYFEEHFKDQKLRALLASQWGDYGLPPAESAFVIHAMIVVHYLAG
                     GYYPVGGAGKIAEAVQEILERTGGGILVSHEVTEVIVEGGRAVGVRARKLPARADSEQ
                     EFRAPVIVSDAGAWNTFLKLVPESVELPFRDSIRSFYDRVPVTTNVTLYLGLKDDPRK
                     LGFRGENYWIYSSYDHDQSFAQRSSWIESGKPPGAYLSFPSLKNRESKSHTAEIIAFT
                     DYGPFEKWKDASWKKRGEEYEAMKEKIAQSLIAYVDERLPGLKSIIDFHELSTPITNE
                     HFTAHPRGSIYGLACVPDRFREEKVAFCRAKTPIEGLFLSGADVSSPGIAGAMMGGFA
                     CLTQIVSGFTILSLFRSRK"
     gene            complement(4875..6275)
                     /gene="mgtE"
                     /locus_tag="PKK76_04030"
     CDS             complement(4875..6275)
                     /gene="mgtE"
                     /locus_tag="PKK76_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNL67663.1"
                     /translation="MDKDPKELAREEEYSFRRDDPAAGDQIETLLDQHRDIELDELLV
                     ELYDADVAYLLQHLDQEQRERLLLRLSPERRGLVLLELDDEERKDLLETLTPRQVAEI
                     VQEQESDVAADIIEDLPEETTTEVISMLPQEDRRDVAELLSYPENSAGALMTKQFASV
                     LETSTVKQAIQSIRRATMQSDDIYTVYVVDAGGHYKGHLALDKLILASSRTRVKKIME
                     TELLPIPVNMDREEVAQMFTRYNFISVPVVDAQDVMVGRITVDDILEVVEEEASEDIL
                     RMGGVSADETLTSPIFQSSVKRNIWLFVNLLTAFLASSVVSLFEATIEKVVILATLMP
                     IVAGMGGNAASQTMAVIIRNIALGDLSPSGLPRALVREATMGAMNGILQGLITGLGVF
                     LFTGKIVLACVISAAMIINMMIAATAGTAIPLVLKKLNVDPAIASSIFVTTFTDVCGF
                     MSFLGLARLMLPYLVS"
     gene            6352..7764
                     /locus_tag="PKK76_04035"
     CDS             6352..7764
                     /locus_tag="PKK76_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67664.1"
                     /translation="MAMFVPPVLYLLSLLLVAYLLRDQDRQDDQYFLAGRTIGSLPAI
                     LSVVANETSVATVMIFPAAGFKSDFVLVWLCLGFILGRTLVAVFFMRKLYENHKLSIY
                     ETIGGTRTGSVPLAFAYVMAKYIANGARFYMAGYALQQLLGWNIAGWIVVVAMIVAVY
                     SLTGGIRAVVITDQIQGSVIYAMGLFLVAYLGYEIYQIGQAGYAHADALGILQRLRLT
                     DARASFTNSDYYLTLLLGGMVISIGSHGADQDMIQRILATKSARRASRSIFISGIGAS
                     IVILTYLAIGVLLREAGHGQLDAKSPLVDYIFHLNHPWLAGCFVVLLLSTGMSSLDSA
                     TNSTGAIWKFITKSNYPGILWSVFSLECLVISALLFALLGRSDFLDLALGSMSYIHGA
                     LIAILITYTFLPRLIHPASIVLAVAGSVGATIVLNLQVHSAWTVQILGSTFTALLLCM
                     AVGWISERVLSRRSRVPGSS"
     gene            complement(7715..8389)
                     /locus_tag="PKK76_04040"
     CDS             complement(7715..8389)
                     /locus_tag="PKK76_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67665.1"
                     /translation="MMKKILIALVVIAAGLYWFTHRATSVNSTDAPGFKPLRDDALAQ
                     KYRPYLLASEFGKPQALYYRASRSGEKLYITYHYVWDREVNNAEGWKPWLSRNLYTGG
                     LALQRTMFGKGDIEMVSLVLDGEGKVESVVYETALGHSPSDFSVKHETIRTARPATPL
                     VFKVISWNHLFLLQEKPPDPSAWLDLDVEYFTQPLWEEYEMFKEKETVLKRNRAHEIY
                     ERAPAH"
     gene            complement(8395..8961)
                     /locus_tag="PKK76_04045"
     CDS             complement(8395..8961)
                     /locus_tag="PKK76_04045"
                     /inference="COORDINATES: protein motif:HMM:NF023164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HNL67666.1"
                     /translation="MFSKIKEVYSRIAPTRTFRIVRALGLALIIGGIWLQFIQPRLPG
                     KKDMPDFEPELAAETGKPNVLDWKNLRELNLKTNQPSAKLKKSDGQVVRIAGFMVPLE
                     DQAEQVSEFLLVPYPQACIHVPAPPPNQIVHVKMAAGKKANMVWWEPIWAEGKLRIVR
                     TANIYTESSFQLSGLKTEPYQDDYDAPH"
     gene            complement(8984..9583)
                     /locus_tag="PKK76_04050"
     CDS             complement(8984..9583)
                     /locus_tag="PKK76_04050"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNL67667.1"
                     /translation="MFTGIVESLGRVVAMVGDENQKTFTIESTFAQELIPGQSVSHNG
                     VCLTVEKIDAKASVYNVTLIRQTLERSSLGALRVSSNVNLERCVRADARLDGHVVQGH
                     VDSVGTIVLAENEPGQKRFRIEFPDKFASLIVPRGSICVDGISLTVANSGPGQVFDVC
                     LIPHTLEVTIASQWEVGTTVNLEFDIIGKYVKKWMDLRG"
     gene            complement(9583..>9782)
                     /locus_tag="PKK76_04055"
     CDS             complement(9583..>9782)
                     /locus_tag="PKK76_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67668.1"
                     /translation="STESESENENGESSGRPGDRTETRTFYFWGDVTDVQLVLTRDWT
                     QVELSFDLPAPQTLSEDQRGH"
BASE COUNT         2423 a         2574 c         2357 g         2428 t
ORIGIN      
        1 gatttgctcg ggcttaccaa ctctcagatt cttggaaaaa caagttttga tccatcatgg
       61 aatgtagtgc acgaagacgg cagcgacttt ccgcctccgg atcatccagt tcctagagca
      121 atccgcgaga agaaagcggt acagaacgtt gtgatgggag tcttccggcc cgcacgacag
      181 gatcgagcat ggttgcttgt aaatgcaatt ccagaaattt ccgctgatgg atccgtagtc
      241 caggttcttt gcacattcac agacatctcc gcgatcaaga cggcagagac agcgctgcga
      301 gacaatgaga ggcagtatcg cggattcatt gagctttcac cggatggcgt agtggttcat
      361 caggaaggtg tgctcgtcta tgcgaaccgt gccatggcgc gaatcctggg tgctcacggg
      421 cccgctgatt tgattggaag gagggttctt gatttagttg cgccagaaat gcagcaagag
      481 atcaaagata gaattcaagg aatactttca gaaggcaaaa tcgcccctct tcgcgaagag
      541 aaactggttc gccttgatgg aggcctcata gatgtagaag cctccggtgc gccgcacaca
      601 tggagaaata aacctgcagt gctcgtagta gtgcgcgata tcacagatcg aaagaaagcg
      661 gaagcacgtg cgaaagaaaa tgaactgctc tacaaatcga tttttgacgc cgccggagat
      721 ggtctgtttc tcgcggatcg aacccctcgc tacgtcgacg taaatcaagc gggcctggac
      781 atgctcgggt acacgaaaga agaactgctt cgcatgggcc ccgcggacct tgtctctaaa
      841 gaagatctgg aaaaaaatcc actgcgaaca aaggatatgt ttgagcaacc gggagtggtc
      901 gtccggcgca agctgcgaag aaaagacggc acaattctcg aggcagaaat caatgcgcgc
      961 ccgatcacac ccgctttgac tctgggaatc gctcgagaaa tgggagcgcg actcgcggct
     1021 gaaatcgcgc tgcgcgactc ggaagaaaaa tggaagctga ttgccgagaa cgttcccgca
     1081 ttcattctac tcgtagatcg tcagggaaaa attctattca tcaaccattt gtttcctggt
     1141 tggaagcgcg aagaaatcat cggaaagcaa atcgctgatt tcatgccacc agaagaacaa
     1201 actctaacac cagtacgcct cgcagacgta tttgagcgcg gcctggaaat cgaatatgaa
     1261 caagccgcag cgggttctcc cggagaagaa cgtcactacg caagcaggat tgttccgatt
     1321 cgccatgaag gggccattac agcggccttg ctcgtagcca cagatatcac agcgcgaaaa
     1381 aaggatgtgg caaggctcga acaccaggct tcgcacgatt cgctgacaga tctaccaaac
     1441 aggcaatttc tcattgaaag cgcgctcgaa gcaattggcc gcgcagaaaa agagggacgt
     1501 cagctggcat tgctcctgat ggatctggat cgattcaaag aaatcaacga cacactgggg
     1561 catttcacag gtgaccttct gctcaagagg attggcccgc gcatggaatc ctatctgaaa
     1621 tccattggag cggagatggc gcgtctgggt ggagacgaat tcggaattct tctccccaga
     1681 atagacgaac ccgcggaagc ggaaggcatt gcacagacat tgcatcacct tttgcgcgaa
     1741 ccattcctgc tcgagggaat gaacctggaa attgactgca gcattggcgt cgctgtatat
     1801 ccgttccatg gtcaggatcc tatttccctt ttgcgttgtg cggatgttgc catgtatctg
     1861 gcaaagaaga agtccgttgg cgtagccatg tatgccccgg atcaggaccg ttattcgcgc
     1921 agaagacttg cacttctcac agagctcggt cttgccattc gcgagagcca gctcactctt
     1981 cattatcagc ccaaatacag tttgcacgat cgaaaactcg ttggagtaga agctttagtg
     2041 cgatggcatc atccagtgca cggaatggtt tctcctggag aattcgttcc tctggcggaa
     2101 actggaaacc tgatcattcc gcttacaaac tgggtattcg aagaagcgtc ccgtcagtgg
     2161 agagtctgga agaacgcggg tcatacggta aagatcgcag tcaatctatc cgtgcgaaac
     2221 cttatggatg agcaataccc gctcacagta gacgcgatac ttaagaaaca taaagtagaa
     2281 agaagctgct ttgagctcga aatcactgaa agcgcaatca tggcggatcc agagagagct
     2341 ctgagcgtat tgaatcgttt gcattcgctg ggtgtggatc tttcgattga tgactttgga
     2401 acggggtact cttcgctcgc ttatctatcg cgcctgccca ttgatgcgct caagatcgat
     2461 ctttcattcg tacgtcagat gatggaaagt gaaagaaaca tggtcattgt gaattcaacc
     2521 gttcgcctgg ctcataccct cggcctcatt gtgattgcag aaggtgtgga agacgaaagg
     2581 acgctcaaca ctctctcgga tatgaaatgc gatatggcgc agggtttctt tttctcccca
     2641 cctcgcagag cagaagattt gactgagatc attgcgcgcg acctctgaaa ggcgcgtcag
     2701 ggtagaagga gcgccggtgt ctggccaagc acgtaagata caccaaaaga aatggaatca
     2761 atcccaggat tctttcttcg cagcccggta aaattcattc ctatatcttc tccgttggaa
     2821 ttgtgcgcat agttcaacgt taggcgccat cgatctgcca ccgggtagcg aagcgacaca
     2881 cccaaatcgt aggtggcccg agtctgccca tcatagacaa agaatcctct tcgcacacgc
     2941 tccgtcactc cacccgcgta ggcaattcca gcataacccc gcgcttccac caaaccaaga
     3001 ttccagccaa gcaagaagcc acttgctccc tggaagatgt gatcatagac agaagccttg
     3061 ccgtaaccgc ccaggatcca gtcatcggaa acattcaatg ttccctggaa caaagcatcc
     3121 gcttttgcca tgggctgcca cagatgctgt ccagcgcatg ttccgcatgt ttgcgccggc
     3181 ccgtagcgaa tctccaattc gtggttctgc aaatcgcgac ttgcgacagg agaaactagc
     3241 gcgaaactcg caactaacag caagtatgcg aatctcattt ccttgagcga aacaaagaca
     3301 aaattgtaaa cccgctcaca atctgcgtaa ggcatgcaaa cccacccatc atcgcgccgg
     3361 caatcccggg agaggaaaca tccgcgccag acagaaacaa tccttcgatt ggagtttttg
     3421 cccgacagaa tgcaaccttt tcttctctaa atcgatctgg cacgcaggcc agcccgtaga
     3481 tcgatccacg aggatgggct gtaaaatgtt cattagtaat gggcgtagat agttcatgaa
     3541 aatcaatgat cgatttcaaa ccaggaaggc gttcatccac ataagcaatc aaagactggg
     3601 cgatcttttc tttcatggcc tcgtattcct cccccctttt tttccaggag gcgtccttcc
     3661 acttctcgaa cggaccatag tccgtaaagg ctatgatctc cgccgtgtgg cttttgctct
     3721 ctctgttttt caaggatgga aaagaaagat acgcaccggg aggtttgccc gattcgatcc
     3781 aggaactccg ctgtgcaaaa ctctgatcgt ggtcgtaaga tgaatagatc cagtagttct
     3841 cgccccgaaa tcctagtttg cgtggatcgt ctttcaaccc gagatacaaa gtcacattgg
     3901 ttgtgaccgg aactctatca taaaagctac gaatgctatc tcgaaatggc agctccacag
     3961 attccggaac aagtttcaga aaagtattcc aggcacccgc gtcagaaaca atcacagggg
     4021 cgcgaaactc ctgctctgag tctgcgcgcg ccgggagctt ccttgcgcga acaccgactg
     4081 cgcgtccacc ttctacaatc acttctgtga cttcgtgaga aacgagtatc ccgcctccgg
     4141 ttctctcaag gatttcctga actgcttccg caatcttacc cgcgcctccc acaggatagt
     4201 agccacctgc aaggtagtga accacaatca tcgcatggat tacaaacgcg ctttctgcgg
     4261 gcggaaggcc ataatcgccc cactgagacg caaggagtgc gcggagtttc tgatctttga
     4321 aatgttcctc gaagtattgt ttcgtggtca tgagtgcgga agaaagcccc agtgaatcca
     4381 ccagatgcgt gagcttatcc aaaaaaggag gcagagactt catggtgatg tggcgaccaa
     4441 accaggaagc agcacgcttg gcatccgcta cgtacttgcg aatggccgct tcttctgctg
     4501 ggaatttttc tatcaaatct gcaatgaatc gttcttcgtt tccgaaaaca ggaaacgtga
     4561 aatcgggata cacgaacttt tcaaaaggct cattcatttt ctcccagtct actttgctgt
     4621 ccgtgatgac atcgaacaat cgacgcatca ttccgccttc ttgcatatcc ccgacatagt
     4681 gaacgccaac atcccagtgg taatgctttc gcgtaaattc gtgagtgaat ccgccagcgc
     4741 gaaagtgtct ttccagcacc agaactttct tcttttcttt ctgcgccagt atgcttgcga
     4801 ccgcgagacc gccgatcccg cttccaatta caattgcgtc atactgattt accgtcatac
     4861 gatcttcctc cgtcttacga aacaaggtag ggcaacatga gtcgggccag tccgaggaaa
     4921 ctcatgaaac cacagacatc cgtgaaagta gtaacaaaaa tgctggatgc gatggccgga
     4981 tcgacattga gtttttttag aaccaatgga atggccgttc ccgctgtggc cgcgatcatc
     5041 atgttgatga tcatcgctgc cgaaatcaca caggccagaa caatcttgcc cgtaaacaga
     5101 aacactccga gtccggtgat gagtccctga agaattccat tcatggctcc catcgtcgct
     5161 tcgcgaacga gcgcacgcgg aagacctgag ggtgaaagat cgcccagagc gatattgcga
     5221 atgattacgg ccatagtctg acttgctgca ttgcctccca tgccagcaac aatgggcatc
     5281 aaggttgcga gtatgaccac cttctcgatg gtggcttcaa atagactcac aacgctcgat
     5341 gctagaaaag cggtgagcag atttacaaag agccagatat ttcgcttaac ggacgactgg
     5401 aagataggag aagtgagtgt ttcatccgca gaaacaccgc ccatgcgcag aatatcctcg
     5461 gacgcttctt cttctacaac ctcaaggatg tcatccacag taatgcggcc aaccatcaca
     5521 tcctgcgcgt ctacgacggg tacggaaata aaattataac gcgtgaacat ctgagccact
     5581 tcttcgcggt ccatgttcac tggaatcgga agcagctcag tctccataat cttcttcacg
     5641 cgagtgcgcg aactggcaag aatcaactta tcgagtgcca ggtgcccctt atagtgtcca
     5701 cctgcgtcca caacatatac agtgtagatg tcgtcgcttt gcatggttgc gcgacgaatc
     5761 gattggattg cttgcttcac agtacttgtt tcaagcacgg aagcaaactg tttggtcatc
     5821 agcgcgccgg ctgaattctc tggataagaa agaagttccg caacatctcg tctgtcttcc
     5881 tgaggaagca tggagatgac ttccgttgtg gtttcttcgg gaagatcttc aatgatgtcc
     5941 gcggcgacat ctgactcctg ctcctgaaca atttccgcaa cctggcgagg agtgagagtc
     6001 tccagtagat cctttctctc ttcatcatcg agttccagga gtaccagacc gcgtctttcc
     6061 ggcgaaagac gtagaagaag cctctctctt tgttcctgat ccaggtgctg gagcaggtaa
     6121 gctacgtctg catcatagag ctcaaccagc aattcgtcga gttcgatgtc tctgtgttga
     6181 tccaggagag tttcaatctg atcgccggca gccggatcat cccgcctgaa cgaatactct
     6241 tcctctcgcg cgagctcctt tggatccttg tccatcttcg cccatcccgt gaccgaggat
     6301 ggtcgtgtca aatttccttt ccttttaatt cgaaaaacga tccatggagc catggctatg
     6361 ttcgtcccgc ccgtattgta tctgctttcc ctcctgctcg ttgcttattt gcttcgagac
     6421 caggacagac aggacgacca atactttctg gcaggccgca cgatcggatc gcttcctgcg
     6481 atcctttccg tcgtagccaa tgagacatcc gttgccacag tgatgatttt tcctgcagca
     6541 gggttcaagt ccgacttcgt tctggtgtgg ctctgtctcg gattcatcct gggacgaacc
     6601 ctggtggcag tcttctttat gcgaaagcta tacgaaaatc acaagctttc catatacgaa
     6661 acgattggcg ggactagaac tggaagcgtc cctcttgcgt tcgcatatgt gatggcaaaa
     6721 tacatcgcaa acggagcgag attctacatg gcgggttacg cgctgcagca attgctcggc
     6781 tggaatatag ctggatggat cgtcgtggtg gcaatgatcg ttgcagtgta cagtctgact
     6841 ggaggcattc gcgcagtagt catcacagat caaatccagg gaagtgtaat ctacgccatg
     6901 ggtctattcc tcgtagccta cctcggctat gaaatctatc aaatcggcca ggcaggttac
     6961 gctcatgcgg acgctctggg aatcctgcag agactcagac tcacagatgc tcgcgcttct
     7021 tttaccaaca gcgactacta tctgactctg cttcttggtg gtatggtgat ttcaattgga
     7081 tcgcacggag cggaccagga catgatccag agaatcctcg cgacaaaaag cgcacgccga
     7141 gccagtcgct ccattttcat ttcaggcatc ggggcaagca ttgtgattct aacgtatctc
     7201 gctatcggtg tgctcttgcg cgaagcggga cacgggcagc tggatgcaaa atcacctctg
     7261 gtagactata tctttcattt gaatcatcct tggcttgcgg gatgttttgt agtgttgctt
     7321 ctatccactg ggatgtcttc cctggactct gccacgaatt ctacgggagc tatctggaaa
     7381 ttcataacaa aatccaacta tcctggaata ctctggtctg tgttttctct tgagtgcctt
     7441 gtcatctcag cgcttctgtt tgcactcctc ggaagatctg acttcctgga tcttgcgctt
     7501 ggctccatga gttacataca cggcgcactc atcgcgattc taattaccta cacgtttctt
     7561 cccaggctca ttcatccggc ttccatagtg cttgctgtgg cagggagtgt gggcgcgacg
     7621 attgtattga acttgcaagt ccactccgcg tggaccgttc aaatactggg ttctactttt
     7681 actgctcttc tgctctgtat ggccgttggc tggatcagtg agcgggtgct ctctcgtaga
     7741 tctcgtgtgc ccggttcctc ttaagaacgg tttctttctc tttgaacatt tcgtactctt
     7801 cccataaagg ttgagtgaag tattccacat ccaggtccaa ccatgcggat ggatctggtg
     7861 gtttttcctg tagcagaaaa agatggttcc acgaaatcac tttgaatacg agaggtgtag
     7921 ccggtcgcgc ggtgcgaatg gtctcatgct ttacagaaaa atcagaagga ctgtgtccaa
     7981 gtgcagtttc atatacaacg ctttccactt tgccttcgcc gtccaggacc agagatacca
     8041 tttcaatatc tcccttgcca aacatggtcc gctgcaatgc cagaccacct gtataaaggt
     8101 tgcgactcaa ccatggtttc caaccttctg cgttgtttac ctcacgatcc caaacataat
     8161 gatacgtaat atatagtttc tctccggatc tgcttgccct gtagtagagt gcctgcggct
     8221 ttccaaattc agatgcgagc aaataaggcc tgtatttctg agccagtgcg tcgtcgcgca
     8281 gaggtttgaa acccggtgcg tccgtggagt ttacggacgt ggcgcgatgg gtaaaccagt
     8341 acaggccggc cgcaatcacg accagggcaa tcagtatctt cttcatcata gtgctcagtg
     8401 tggagcgtcg tagtcgtcct gatatggttc tgtcttcagc cccgacaact gaaaagagga
     8461 ttcagtgtaa atgttcgcag tgcgaacgat tctcagcttt ccttctgccc agattggctc
     8521 ccaccacacc atgtttgctt tcttccctgc cgccattttc acatgaacga tttgattggg
     8581 tggtggtgca ggaacgtgga tgcatgcctg cggatacgga accagtagaa attcacttac
     8641 ttgctccgcc tgatcttcca ggggaaccat gaaaccggcg attcgcacca cctgtccgtc
     8701 cgatttctta agttttgcag atggttgatt tgttttaaga ttcagctcac gtaagttttt
     8761 ccagtcgagc acattgggtt ttccagtttc tgccgccagt tctggctcaa aatcgggcat
     8821 gtcctttttg cccggcagac gcggctgaat gaattgcagc cagataccgc caatgatcaa
     8881 tgcaaggccg agcgcgcgca cgatgcgaaa ggttctagtg ggcgcgatgc gagaatagac
     8941 ttctttgatt tttgaaaaca tctctggttt cagagaaaag agatcagcct cgcaaatcca
     9001 tccatttttt tacgtacttt ccaatgatgt caaattctag attcacggtc gttcccactt
     9061 cccattgcga agcgatcgtc acttccagag tgtgcggaat caaacaaaca tcaaaaacct
     9121 gacctggacc cgaattcgcc acggttagac taatcccatc cacgcaaatg cttcccctgg
     9181 gaacaatcaa tgatgcaaat ttgtccggaa attctatcct gaaacgtttc tgccccggtt
     9241 cattctctgc gagaactatc gttccaactg aatccacatg cccctgcact acatgtccat
     9301 ccagacgagc gtccgctctc acgcaacgct ccagattcac attcgaagaa actcgcaagg
     9361 ctcccagcga actccgttcc agagtctgac ggatcaatgt cacattgtat acagatgctt
     9421 tcgcatcgat tttctctacg gtcagacaca ctccgttgtg cgagacgctt tgccccggga
     9481 tcaattcttg tgcgaaagta gattcaatcg tgaatgtctt ctgattttca tcccccacca
     9541 tcgcaaccac gcgacccagg ctttctacaa ttccagtaaa catcaatgcc ctcgctgatc
     9601 ttcagatagc gtttgtggcg ccggaagatc aaacgataat tctacctgag tccaatctcg
     9661 cgtcaagacc agctgaacgt ctgtcacatc cccccagaaa tagaaggtgc gcgtctccgt
     9721 gcgatcaccc ggccgacccg atgattctcc gttttcgttt tcactttcgc tttctgtaga
     9781 tt
//
LOCUS       DAORFF010000062         9746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3245_length_9746_cov_3.454545, whole genome shotgun sequence.
ACCESSION   DAORFF010000062 DAORFF010000000
VERSION     DAORFF010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9746
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3245_length_9746_cov_3.454545"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PKK76_04060"
     CDS             <1..564
                     /locus_tag="PKK76_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNL67669.1"
                     /translation="VSHLAQGPLSVLGYLFKTYGFSGALARMKKAKQSFGRPFTGFAT
                     EKFYTAAPIKCGPYACRVRMLPASDKPDPNASQSWAKDIQKRVSSGALVHDFQLQFFV
                     DEKTTPVEDASVDWPESESPYVTVARLTIPQQAVEGPEVQKLQDEIDKAAFDPWGALE
                     EHRPLGDVMRARKYVYFASQKTREATH"
     gene            complement(611..1918)
                     /locus_tag="PKK76_04065"
     CDS             complement(611..1918)
                     /locus_tag="PKK76_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNL67670.1"
                     /translation="MLTRPFVQISNGLKWMKDPCSLLDLMAARHGLAFRLDLPILGNC
                     LITGDPDLLAAIKNHPNLEAGKGIRALRRILGDGSLITLDGEAHATRREIIAKHFRAD
                     QSQAVDALTVDVMRSLYEDLPDGDFSAYSLFHEASLLVILRFLFEDLTLKELEDLRSD
                     TLLFLSAFSSAVVLFVRPLQIDHILFPWGRALNHRRNLEKSILKFSRRQNRASQIFAE
                     MEEAGSTNETACAEILTLLMFGHDTAAAAMSWTTSLLACHPEVLEKTREDAAIRKRKQ
                     SSVQQISSLQSASLYSAEDFVHGSILESLRLRPVVIHLSRVAQERIELGEYALLPGEF
                     VLPCQYVAHRNENIFKDATSFLPQRWIPDIAKQEETKLTSAFFPFGFTDRLCPGMPFA
                     LRQMQIQLQTVLEYSWAAKKDPEPRRQMVLVVPESGGTLVKLK"
     gene            complement(1912..2724)
                     /locus_tag="PKK76_04070"
     CDS             complement(1912..2724)
                     /locus_tag="PKK76_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67671.1"
                     /translation="MSADLYEHVDKECFRLYVRFIRRHIRYAPIVIALYGYSLFQWKK
                     ARPLRAGFALAQAVDDMLDGDWQIQSDPIELTRLCVRDPSAVHDDGLKQICVYLIPWM
                     KEHGLLDSLSSLFETLILDCKRRSTREQWPGETLKNHHFRTFELSAKIALGLCELELS
                     PADARGVIESLAWVSPMRDLREDLEKGINNIPSEVVDTSRMTAKEALSNRRVIEWMKT
                     EYQSALLSLDRSNLELKRIQDKKGAVYFRIFHRLVKSYARKYPRKYPEILSC"
     gene            complement(2731..4911)
                     /locus_tag="PKK76_04075"
     CDS             complement(2731..4911)
                     /locus_tag="PKK76_04075"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNL67672.1"
                     /translation="MLRRHFRLKAKVALLLAVGIPGPYFLLQYFPILQATPAPSIFLD
                     GKIPFIPETMWIYQTLYPCLLLVGFSLFRRHDVIRYSWTLLLASVIADFVFLIHPTFV
                     PRPDFAQGLYGIWTQIERPMNAIPSLHAAFIAISWNYAGTKSRSSMRPAQVLFSVWMI
                     VILISTIATSQHRWLDLAVGLLLGQFCSLLIRRPHPARSVLIVTGSLLEPSGSIAGAL
                     LRMLAQRSSEKHGMFSLKIKAVIGEIFVYGILERLGRQRYAAYISEYFKGPPSPPDLT
                     HSLLETMLRKEGIPTDHITVAQVFANPFRTARLLRKHSCIFLSSTLLHDQSELTTVAS
                     ALKRPWNRVVVGGALTGSLPTTFGAAHIDMLAPGYGEILVPALAPWIQSGFRNHAFES
                     LRVRRIQSMIRVEAFPPSSRDLDFLPSPDFHGQPETIYYESVRGCPYRCSFCNYPYLF
                     SDANFRFKSARKIYDDWMQYAGSGVRTIISLDSLFTIPRKRLDQLCNSLIRAGSPVRW
                     ICYVRADDIDLERAQMMREAGAFQVHIGIESGDDAILANMNKKTSAEVNARAIHNLRR
                     AGITSIATLIVGFPGETEQSIQTTLNFLQQNPPDFHFLAAFSTRVPGVPVLSEESKSR
                     FGLVVNTELATMSPYWSHATMDCAKAGVFLRNATKYLIENRISLDAALFHKGLSGYDP
                     AHRAELLDFQEAAIRRYTTLRRFMDAVHQKIDHALARDMAAFFQ"
     gene            complement(4926..5831)
                     /locus_tag="PKK76_04080"
     CDS             complement(4926..5831)
                     /locus_tag="PKK76_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNL67673.1"
                     /translation="MIQSPRLIPGRTNAIALGLLFPTLLLLLMFGPGTHGITFAVATV
                     AFSILGFTAYCFLHEAEHELLFESRFLNDGAGVMLGLFFPAPFHLLRQGHLGHHARNR
                     TDTEAFDLYHPNRPREKWMRRFQLYGTLTGIWYLVVVLSNVLAFTWPLPLLFRRTDRS
                     ARALLSSFNPEKIGIVRLEALLALLFHGTLLWLSDSPGFYLLMYFFSGLLWSSQQYLH
                     HYGTERHPERGARNVRTWKFLDLLWLNHNLHLTHHLKPSLPWIHLRLNGPPATTTMMS
                     AYWQMWRGPVADASTVMDATEGRVV"
     gene            complement(5828..6286)
                     /locus_tag="PKK76_04085"
     CDS             complement(5828..6286)
                     /locus_tag="PKK76_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67674.1"
                     /translation="MDQTNELPPILKDQRKTDVEHLRLLSIFHYVNAGLTATGLLFLA
                     GHYAIMHTVFTNPALTKSPKGDGPPLEFFKVFLWFYVLGAVFLTVTATLNVLSAYFIQ
                     KRINRVFSIIVAGLNCVLSMQCPIGIGLGVFTFVVLFRESVRELYDVAAR"
     gene            6392..8125
                     /locus_tag="PKK76_04090"
     CDS             6392..8125
                     /locus_tag="PKK76_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNL67675.1"
                     /translation="MIQSNYFADNPDLQDHVERFVDWQEMIGLYEDGFRDAQEYKATG
                     NERLSMAPSTNEEALAYYHQMLDAYGDMCGNELSQLAAPMDRIGLKFENGKVTHPAEI
                     EKLFEKFDDAGLRPIAFQKKYGGLGVPNVLKALCQEMSYRADGALSITCGSVNLAAIL
                     EAYASKEMRDEWIPKLIHERYSVTMGLSEADFGSDLPSVRTRAEKIDGKWYLTGTKRF
                     QTMACGLNKYPAVLLALARTGAPDSGARGLSFFLVDSKDFEVTGIEKKLGIKASPTCE
                     VAFEKAPGLLIGEEGHGLSRYVIGMLNGARLSVASQGTGIATAAWYEARKYASERIQF
                     GKPIDQIPAVAKMLRRMERESKAMRCLMVEAASAVDRYHWKALRAIDSDEKTKKDDFG
                     RKWEKLASTITPMAKYYISEMANSVAYDAIQVFGGAGFIEEYDVARLYRDARITNIYD
                     GTTQIQVNAAIGGVVSGMAETGGLRAHLQEMLDLVAKGESSRAAILKELFAEFESLVS
                     HFKTLEGAKKDSFAFEVVETATRTLNSLLLAKTSLKLTGTAREERFQLTHEYAVESLG
                     TAQASKLRMTR"
     gene            8168..8374
                     /locus_tag="PKK76_04095"
     CDS             8168..8374
                     /locus_tag="PKK76_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNL67676.1"
                     /translation="MKFELKGHEYIQLNQLLKFLGLVETGGEANQCIVDGQVLFNGSV
                     ELQKRKKVRRGDTVEFQSKKIEII"
     gene            complement(8514..9218)
                     /locus_tag="PKK76_04100"
     CDS             complement(8514..9218)
                     /locus_tag="PKK76_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNL67677.1"
                     /translation="MRILIVEDEPIHARYLSHTLKEIPATAEAVVHTQKTLTAAECYL
                     LDHPVDLLFLDLNVYDGDGFTLLQGFAGAQFATIVVSAHPERAIEAFECGVLDFLPKP
                     ITKARLLTAIERYEKHRYDSRHTLKFFSIYADGVLETLPLAEISHFEAADKQVIIHQN
                     HGRKRTCTRKLGDVAKVLPPRFIRVHRSFIADTGQIKTLQAAGGGRYDLTMQSGVTIP
                     VSRSVYASLKKNLMQS"
     gene            complement(9199..>9746)
                     /locus_tag="PKK76_04105"
     CDS             complement(9199..>9746)
                     /locus_tag="PKK76_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNL67678.1"
                     /translation="AAIRGWLLENPEKSANLLDDFAGEIALIQAMVGQKDVPLADEIR
                     LCRLHLHVMEARREKIYSFRARGMTGHERVPPMIFHTMLENAFTHLDTENGSLRFSLR
                     KTSSKSTTEVVSRFAFAVSNHDAGSIRQRKRGNGIGLRYVQARLEESFPGSWTLEQKR
                     CRKGYLIRINIKEGADANTDR"
BASE COUNT         2500 a         2326 c         2628 g         2292 t
ORIGIN      
        1 gtttcgcatc tcgcgcaggg tcctctgtct gttctcggat acttattcaa gacatatggt
       61 ttcagtggag ctctcgctcg catgaagaag gcaaagcaat cattcggaag accgtttacc
      121 ggatttgcga ctgagaaatt ctacactgcg gctccgatca aatgtggtcc gtatgcctgt
      181 cgcgtgcgta tgcttcctgc cagtgataag ccggatccga atgcaagcca gagctgggcc
      241 aaagacattc agaagagagt ttcttccggt gcactcgtac atgatttcca gctgcaattc
      301 ttcgtggatg agaaaacaac tccagtagaa gatgcatctg tggattggcc ggaatcggaa
      361 tcgccttatg tgactgtagc aagattgaca atacctcagc aagcggtaga aggccctgaa
      421 gtacagaaac tccaggacga aatagacaaa gccgcgtttg atccatgggg cgctctcgaa
      481 gaacacagac cactcgggga cgttatgcgc gctcgcaagt atgtttactt tgcgagtcag
      541 aagacccgag aagctactca ctaacgttag acgatgttga tactcgcact ggcccttccg
      601 gtgcgagcgt ctatttgagt ttgaccaggg tgcctccgct ctctggaacc acgagaacca
      661 tctgcctgcg aggttccggg tccttttttg ccgcccagga atactctaaa accgtttgga
      721 gttgaatttg catttgcctg agcgcgaaag gcatgcctgg gcaaagtctg tctgtaaaac
      781 cgaacggaaa aaatgcactc gtcagttttg tttcttcctg cttcgcgatg tccggtatcc
      841 atctctgcgg cagaaaggaa gtcgcatcct taaaaatgtt ttcgtttctg tgggccacat
      901 actggcaggg cagtacgaac tcaccgggta agagggcata ctcgcccagc tctatccttt
      961 cttgcgccac gcgactcagg tgaattacaa ccggcctcag gcgcagactc tctagaatcg
     1021 agccgtgcac aaagtcctcc gcgctgtaga gtgatgcgga ctgcagagaa gagatctgtt
     1081 gaactgagga ttgctttctc ttgcgaatgg ctgcatcttc gcgcgtcttt tcgagaactt
     1141 cagggtggca tgccagtaga gatgtggtcc aggacatcgc agctgccgcg gtgtcgtggc
     1201 cgaacatgag cagagtgaga atttcagcgc aggcggtttc atttgtggat ccagcctctt
     1261 ccatttcagc aaagatctgg gacgcccggt tttgccttcg cgagaatttc aggatgctct
     1321 tttccagatt tcgcctgtgg ttcaatgccc ttccccaggg aaaaagaata tggtcgattt
     1381 gaagaggacg aacgaataaa accacggcgc tgctaaaagc agacaaaaaa agcagagtat
     1441 cgcttctcag atcctccaat tcttttaagg ttagatcctc gaacaaaaag cgaagtatga
     1501 cgagcaagga tgcttcatga aacaaagaat aggcactgaa gtctccgtct ggcaaatctt
     1561 catataaaga gcgcataacg tccactgtga gcgcgtccac agcctgggat tgatctgcac
     1621 gaaaatgttt cgcaatgatt tcgcgtctgg ttgcgtgggc ttctccgtcc agtgtgatca
     1681 gagacccgtc cccgagaatt ctccggagcg cccggattcc ctttccggcc tcgaggttgg
     1741 gatgattctt gatggcggcg agaagatccg gatctcccgt tatgaggcaa ttgcccagaa
     1801 tgggaaggtc cagtctgaag gcgagtccat gtctggcagc cataaggtcc aggagcgagc
     1861 acggatcctt catccatttc aggccattgg agatttgaac gaaagggcga gtcagcaact
     1921 gaggatctcc ggatacttac gcggatattt cctggcgtag cttttcacca gtcgatggaa
     1981 gattctaaag tacacggcac cttttttgtc ctgtattctt ttgagctcca gattcgatcg
     2041 atccagcgac aggagtgcac tctggtattc cgtcttcatc cattcgatca ctctcctgtt
     2101 ggaaagagct tcttttgctg tcatacgcga agtatccact acttcgctgg gaatattatt
     2161 gattcctttt tctaagtctt cgcgcagatc gcgcatgggt gatacccagg ccaggctttc
     2221 aatgacgcca cgcgcgtccg ccggtgaaag ttcgagctca cagagtccaa gtgcaatctt
     2281 cgcggaaagt tcaaatgtgc gaaagtgatg gttctttagt gtttcgccgg gccattgttc
     2341 gcgggtggat cttcttttgc agtcaagaat gagcgtttcg aaaagactgg aaagggaatc
     2401 cagcagacca tgttctttca tccagggaat caaatagacg caaatttgtt ttagcccatc
     2461 atcgtgtacg gcggaaggat cgcggacgca caaacgtgtg agttcgatcg ggtctgattg
     2521 gatttgccag tccccgtcta acatgtcgtc cactgcctgg gccaatgcga atcctgcgcg
     2581 aagagggcga gctttcttcc actgaaagag ggaatatcca tacaatgcga tgacaatcgg
     2641 agcataacgg atgtgccttc ggatgaatcg cacgtaaagg cgaaagcatt ctttgtccac
     2701 atgttcgtaa agatctgcgc tcatggtccg ttactgaaaa aaagcagcca tatctcgagc
     2761 aagagcatgg tctatcttct ggtgcactgc atccatgaag cgacgcaacg tcgtataacg
     2821 tctgatcgct gcttcctgaa aatccagcaa ctccgccctg tgcgctgggt cgtagccaga
     2881 aagacccttg tgaaacaagg cagcatcgag ggagattcga ttttcgatca ggtatttggt
     2941 cgcgtttcgc agaaacactc cggccttcgc gcagtccatc gttgcatgag accagtacgg
     3001 tgacatggtg gcaagctccg tgttgaccac caggccgaat cgacttttgc tctcctcaga
     3061 aagaacagga acgcccggta cccgcgtgct aaatgctgcg agaaaatgaa agtccggagg
     3121 attctgttgc aggaaattta gagtcgtctg aatggattgt tccgtttctc cggggaaacc
     3181 aacgatcaac gtggcaatgc ttgtgatgcc ggcgcgtctg agattgtgga tggcgcgcgc
     3241 atttacttct gcactagttt ttttgttcat gttggctaga atcgcatcgt caccggattc
     3301 aattcctata tggacctgga acgcacctgc ttctcgcatc atttgcgctc tttccagatc
     3361 gatgtcatcc gcgcgaacgt agcagatcca tcgaacggga gagccggccc gaattagaga
     3421 gttgcatagt tgatcgagtc gttttcgcgg gatcgtaaaa agggagtcaa ggctgatgat
     3481 cgtacgcact cctgacccgg catattgcat ccagtcgtca tagatctttc ttgctgactt
     3541 gaatctgaaa ttcgcgtcgc taaacaggta agggtagttg cagaagctgc agcgataagg
     3601 acacccccgt actgattcgt agtagattgt ttctggttga ccatgaaagt caggcgaagg
     3661 caagaagtcc aggtctcgcg aggaaggtgg aaatgcctct actcgtatca tggattgaat
     3721 gcgcctcact ctgagcgatt cgaatgcatg attcctgaat ccagattgga tccacggtgc
     3781 aagggccgga actagaatct ctccataccc aggggcaagc atatctatgt gagcggcacc
     3841 gaaggtggta gggaggctgc ccgtcaaagc gccccccacc acgactcgat tccaggggcg
     3901 cttgagtgcg gaagctactg ttgttaactc actctggtcg tgcagaagcg tggatgaaag
     3961 aaatatacag gaatgctttc ggagaagccg ggccgttcga aatggatttg cgaacacctg
     4021 cgcgaccgtt atatggtctg tggggatgcc ctccttgcgc agcatggttt ccaggagtga
     4081 gtgggtcaag tcaggaggag atggcggtcc tttaaaatat tctgatatgt atgccgcata
     4141 acgttgcctg ccgagtcgct ccagaatccc gtagacaaaa atctctccaa tgactgcttt
     4201 gatcttgagt gaaaacattc cgtgcttttc cgaagatcgt tgtgcaagca ttctgagaag
     4261 agcgcccgca atggaaccgg acggttcaag caggctcccc gttacaatca gtacagaccg
     4321 cgccgggtgg ggccgacgga tcagaagaga gcagaattgt cccagcaaaa gaccgactgc
     4381 aagatccaac catcggtgct gagaagtcgc aatcgtactg atcaggatca caatcatcca
     4441 gacggaaaaa agcacttggg ccggtcgcat cgaactcctg gatttggtcc ctgcgtaatt
     4501 ccacgaaatt gcgatgaagg cggcgtgaag agacggaatg gcgttcatcg gtctttctat
     4561 ctgcgtccag attccataga gtccctgtgc aaagtccggt cgcgggacaa acgtgggatg
     4621 aatgagaaac acaaagtccg cgatgacgct tgccagaaga agagtccagg aatagcgaat
     4681 cacatcatgt cttctaaaga gcgaaaaccc gacgagcagt aagcatggat aaagggtctg
     4741 gtagatccac atggtttccg gaatgaacgg aatcttgcca tccaggaaaa tagaaggcgc
     4801 gggtgtggcc tgcagaatgg gaaagtattg tagtagaaaa tagggccctg gaattccaac
     4861 agccaggagc aaggccactt ttgctttgag acgaaaatgc cgtcgtaaca ttcgcacgat
     4921 ccgtctcaaa cgactctacc ttcagtagca tccatgacgg tgctcgcgtc cgctacaggt
     4981 cctcgccaca tttgccagta ggcactcatc atagtcgtgg ttgcgggagg tccgttgagt
     5041 ctcaggtgaa tccagggcaa ggacggtttc aggtgatgcg tgagatgcag attgtgattc
     5101 aaccagagca gatccaaaaa cttccatgtc cgcacattcc tggcgccgcg ttccggatga
     5161 cgctcagtgc cgtagtgatg aaggtattgc tgtgaagacc acaaaagacc ggagaagaaa
     5221 tacatcagca aatagaaacc gggggaatcg cttaaccaga gtaaggtgcc atggaacagg
     5281 agcgcaagca aggcttccag gcgaacgatt ccaatctttt ccgggttgaa tgaagacaac
     5341 agagccctgg cactgcgatc ggttcgtcga aatagaagcg gcaagggcca ggtaaatgcg
     5401 agtacatttg acaggactac caccagatac cagattccag tgagcgtacc ataaagctga
     5461 aatcttcgca tccatttttc tcgaggtcta tttggatgat acaggtcaaa cgcttccgtg
     5521 tccgtacgat tgcgcgcatg gtgacccaga tgtccctgtc tgagaagatg aaacggcgcg
     5581 ggaaaaaaaa gtccgagcat cactcctgcg ccatcgttga ggaagcggga ttcgaaaaga
     5641 agttcgtgtt ctgcttcatg caggaagcaa tacgccgtga aaccgaggat ggaaaaagcg
     5701 actgtggcga cggcaaatgt gattccgtgt gtgcccggcc caaacatcaa aaggagcagc
     5761 aatgtcggga ataggagccc cagcgcgatt gcattcgtgc ggccaggaat cagccgcgga
     5821 gattggatca tctggccgcc acatcatata gctcgcgaac agattcgcgg aacaaaacaa
     5881 cgaacgtaaa aaccccaagg ccaatcccaa tcgggcattg catcgagaga acgcagttga
     5941 gtcctgccac aatgatagaa aaaacacgat tgattctttt ctgaatgaag tatgcggaaa
     6001 ggacattgag agttgcagtc acagttaaga atactgcccc tagaacatag aaccacaaga
     6061 aaaccttaaa gaattctaat ggtggtccat ctccttttgg actcttcgtc aaagcaggat
     6121 ttgtgaaaac agtatgcatg attgcgtagt gtcccgcgag gaacaacaac ccggtggccg
     6181 tcagtccagc gtttacgtag tgaaagatgg aaagaagccg caaatgttcg acatccgttt
     6241 ttcgttgatc tttcaaaata ggtgggagct cgtttgtttg atccaccagc agatttcgcc
     6301 ctgtgagcgc ttcgtctgca agtgcttttt acccggactg gatccagaaa aaaaggatac
     6361 tcacaagcgc aatcttcgcc agttttgccg aatgattcaa agcaattatt tcgcagacaa
     6421 tcctgattta caggaccatg tagaacgctt cgtagactgg caggaaatga tcggtttgta
     6481 cgaagacggg ttcagggacg ctcaggaata caaggcaacc ggaaacgaac gtctgagtat
     6541 ggcaccgtcc acgaacgaag aggctcttgc ttactaccat cagatgctcg acgcttatgg
     6601 cgatatgtgt ggcaacgagc tctcgcagct ggcggctccg atggatcgca ttggtctgaa
     6661 attcgaaaac gggaaggtca ctcatcctgc ggagattgaa aaactattcg agaaatttga
     6721 tgatgcgggt ctgcggccca tcgcgtttca gaaaaaatac gggggcctgg gtgttcctaa
     6781 cgtccttaag gcactctgtc aggaaatgag ttatcgtgcg gatggagcgc tcagcattac
     6841 atgcggatct gtgaatctgg cagcgattct agaagcatat gcgagcaagg aaatgcgcga
     6901 cgaatggatt ccgaaattga ttcacgaacg ttattcggtc accatgggcc tcagtgaagc
     6961 agactttggg tccgacttac cgtcagtgcg tacgcgcgct gaaaaaatag acgggaaatg
     7021 gtatctgacc ggaaccaagc gttttcagac tatggcctgt ggactcaata aataccccgc
     7081 ggttctactt gctcttgctc gcaccggtgc gcccgatagc ggtgctcgtg gcctgtcttt
     7141 cttccttgtg gatagtaaag attttgaagt aaccggaatt gaaaagaaac tgggaatcaa
     7201 agcatcccct acatgcgaag ttgcttttga aaaagcgcca ggcctgctca tcggcgaaga
     7261 aggacatggt ctttctcgtt atgtgatagg catgctcaat ggagcgcgcc tgagtgtagc
     7321 ttcgcagggg acaggcattg caacggccgc ctggtacgaa gcgcgcaaat atgcaagcga
     7381 gcgaattcag tttggaaaac caatcgatca gattcctgca gtcgcgaaaa tgctgcgaag
     7441 aatggagcgc gaatccaaag ccatgcgatg cctgatggta gaagcagcca gtgcagtgga
     7501 tcgctaccac tggaaagctt tgcgcgcgat tgattcagat gagaagacaa agaaagacga
     7561 ttttggacgc aagtgggaaa aactcgcgag cactatcact cccatggcaa agtactacat
     7621 ttctgagatg gcaaactccg ttgcgtatga tgccattcag gtatttggcg gtgctggatt
     7681 catcgaagaa tatgatgtgg cgcgtctgta cagagacgcc aggatcacga atatttacga
     7741 cggcaccacg cagattcaag taaacgcggc gattggcggc gtggtgagtg gaatggcgga
     7801 aacaggcggc ctgcgggcgc atctccagga aatgctggat ctagtagcga aaggggaatc
     7861 ttcccgcgct gcgattttga aagaattgtt tgctgaattc gaatcactgg taagccattt
     7921 caaaaccctc gaaggcgcaa aaaaagatag ttttgccttt gaagtagtgg aaactgctac
     7981 acgcactctc aattcacttt tgctcgcaaa gacatcactg aagcttaccg ggacggcccg
     8041 tgaagaaaga tttcagctaa cacatgagta tgcggtcgag agtctgggta cggcgcaggc
     8101 atccaaactg cgtatgacca gatgagttgt cgcggcgaat gagcttgatt ctggcactgc
     8161 ttcgagtatg aaattcgaac tcaaaggtca tgaatacatc cagctcaacc agctcttgaa
     8221 gtttctggga cttgtggaaa ctggagggga ggccaaccag tgcattgtgg acggacaggt
     8281 cttgttcaac gggagcgtag aacttcagaa aagaaagaaa gtgcgccgtg gagacacggt
     8341 ggaatttcaa tctaagaaga ttgagattat ttagaagctg cgcgcagagc ggaataatta
     8401 caccctgacc gcgaccgatg tcggtggggg gccacggctc ggaaacggga caccctccag
     8461 gatgtgtaaa ggtgatgttg cggattaccc gactacgacg ggtttcgctt agatcacgac
     8521 tgcatcagat tcttttttaa cgacgcatag acagagcggc ttactggaat tgtaacgccg
     8581 ctttgcatgg tcagatcata acgtccgccg ccggcggcct gtagcgtttt gatctggccg
     8641 gtgtctgcaa tgaaagagcg gtggacgcgt atgaaacgcg gcggaagaac tttcgcaacg
     8701 tcgcctaact ttcgcgtgca cgttcgcttg cgaccgtggt tttgatgaat gatcacttgc
     8761 ttgtctgccg cttcaaagtg tgagatttcc gcaagaggta gtgtttcgag tacgccgtct
     8821 gcgtagatgg aaaagaattt cagcgtgtgc ctggaatcgt agcggtgttt ttcatagcgt
     8881 tcgattgcag tgagcagacg tgcctttgtt atgggctttg gaaggaaatc cagaacaccg
     8941 cattcgaatg cttcaatggc tcgttcggga tgtgcggata caacgattgt ggcaaactgt
     9001 gcgcccgcga atccttgcag tagagtgaat ccgtctccat cgtagacatt tagatccagg
     9061 aaaagaagat ccaccgggtg atcgagcagg taacattccg ccgcggtgag tgtcttctgt
     9121 gtgtgcacta ctgcttccgc ggttgccggg atttctttaa gagtatgact caaatagcgc
     9181 gcatggattg gttcgtcttc aacgatcagt attcgcatcc gcaccctcct tgatgttgat
     9241 cctgataagg taaccctttc tgcatcgttt ctgctcaagt gtccaggacc cggggaagga
     9301 ctcttcgagc ctggcctgaa cataacgcag gccaattcca tttccgcgct tacgttgccg
     9361 aatcgagcct gcatcatggt tgctcactgc aaatgcaaag cgagacacga cctcagtggt
     9421 tgatttcgag gaagtctttc gcagtgaaaa gcgaagcgaa ccattctctg tgtccagatg
     9481 agtgaatgcg ttttcgagca ttgtatgaaa gatcatagga ggaacgcgct catggcccgt
     9541 cattcctcgg gcacggaaag aatagatctt ctctcttcgc gcttccatga catgcaaatg
     9601 gagtctacaa agacggattt catctgccag tggcacatct ttctgaccga ccatagcctg
     9661 gatgagagcg atttcgcccg cgaaatcatc aagtagattg gcggacttct cgggattctc
     9721 aagtagccag ccacggattg ccgcca
//
LOCUS       DAORFF010000063         9668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3281_length_9668_cov_3.890461, whole genome shotgun sequence.
ACCESSION   DAORFF010000063 DAORFF010000000
VERSION     DAORFF010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9668
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3281_length_9668_cov_3.890461"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..2683
                     /locus_tag="PKK76_04110"
     CDS             263..2683
                     /locus_tag="PKK76_04110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL67679.1"
                     /translation="MRISFGLKLAFATSMLAFVTTSAGLGYFYKITRQNVWNELAERL
                     ADMGRTGQFIFSAEHRRAIDRLNELSEKDSSRNKDHLSIGPGESISVISGSRVHEIEK
                     SDDFILVVQALRQIKNASRKGMVNLGILGQEPWPKDDPPRVRFTYLLVPIPEVKDRSI
                     LKFIADADYEAGDENGNGKIDPEEEASEIGKLYNVSAQPGLMAAFDGKVRTSEEYYED
                     QWGKWMSAYVPILRADGSVAAVMGIDMSATSQFNLLEKLYYICIAVIGGSVVLAIILS
                     LALSRLIASPIVALRAGAERVKARDYSTQIVVKSKDELGILASTFNDMVREVRTYATT
                     LEDRVKERTAELNESRGQMSDILRNIQEGILTIDRSGKVNSEYSGRVPDMLGKQPEGL
                     EFADLFPAQLQNSVRDFVGQLIENPFMSMRMFAAANPLSEVRILSGDQEKVLSFSFSR
                     VKNGAGQIEKLLVVIDDRTEQMKLERALAEREAEQSARIEKLYQILNLDPNVFKSFLE
                     EAGRTADVVVEKLALAGSDRSALDLCFREVHTLKGNARALGLDAIAKNAHELEDVLDA
                     IRKEPESPAQDRIASAGELGKKLIAEIQDGGTLFDKIVNMKDVFRVKTLDASAEIAER
                     CERIVEQEALAAGKAVAFEFENRAGVLRGDALVRVRAMLGHMIRNAVVHGIETPDARK
                     EKGKAIDGSIRLLMTHNDGQVRIAVEDDGAGLDQERIARHAIEQGILTQEQAGVASPA
                     EVLRLIFRPGFSTAERVTESAGRGVGLDVVEEEARALGGNVRVETKRGTFTKFIVVLP
                     ESVLEKRD"
     gene            2724..3089
                     /locus_tag="PKK76_04115"
     CDS             2724..3089
                     /locus_tag="PKK76_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL67680.1"
                     /translation="MKILVVDDSAMMRSVIKSTLASYGSGKQIEVVEAVNGQEALDKL
                     ASESIDLVLLDWQMPVVDGLSFVKQVRGSGKDVPIVMVTSVTDKEKVLEAGKAGVNTY
                     IEKPVRGSVLWENIQEFLH"
     gene            3103..3561
                     /locus_tag="PKK76_04120"
     CDS             3103..3561
                     /locus_tag="PKK76_04120"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNL67681.1"
                     /translation="MNTSHILAFSTCLKTTFRDMLSVDMTQGPIAEHVDYPVRADVSA
                     CITISSKAPACVVLAFDADSAMDAAERFTQTKSPFEDPMVGDTAREILNILVGSAQRE
                     ISEKFEFSLPISIQGRNHEVSCFRKGRNIFCDLHWGTGKKATLFLNLPAL"
     gene            3630..4274
                     /locus_tag="PKK76_04125"
     CDS             3630..4274
                     /locus_tag="PKK76_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNL67682.1"
                     /translation="MITKLCQGFVMFSFIPKEEKYFRSLSELAARVRAGSEIFLRLFD
                     DFGNHHKFADEIKSVEVQCDTIAASITSRLNTSFITPIDREDIFLLVKEVDDIMDAIN
                     GLAFRFDTYSVKTPRSEAVEFARLIHQGAVELEHAFSMLEQNKNLGDHPKNINDIEKK
                     ADHLYRDAIRRLFTEEKDPIEVIKWMSIFETLEDATDRCKDVAEALEGVVVKNK"
     gene            4280..5323
                     /locus_tag="PKK76_04130"
     CDS             4280..5323
                     /locus_tag="PKK76_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNL67683.1"
                     /translation="MNAVLVFVIIFVVIALAFDYINGFHDAANSIATVVSTRVLTPGR
                     AVLWAAFFNFVAFAIFGTHVAKTIGGDMVNIGLIQDDFAFRKLWVLLCGVIGAIIWNL
                     ITWYYGLPSSSSHALIGGYAGAAITANGGFSNGILISAGWIKTVSFIIISPLVGIILG
                     FLIMVAIMWIFRKFSPDRVDRLFRKLQLVSAALFSLGHGGNDAQKTIGIIAAALAASN
                     LVPDAAKNVPFWVVLICYAAIGLGTLSGGWRIVHTMGSRLTKLKPVGGFAAETAGAAA
                     MFMATYGGVPVSTTHTINGSIIGVGATRRLSAVRWGVARSVVWAWILTIPMSGGMAAL
                     LFTLVQLGYKLSA"
     gene            complement(5326..7044)
                     /locus_tag="PKK76_04135"
     CDS             complement(5326..7044)
                     /locus_tag="PKK76_04135"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNL67684.1"
                     /translation="MRFIALILISVTGFCRQSYAGFVAETLSSDSMLGRESGTPSAKK
                     TADWIRDQYIAMGAYPAFASGYTQEFSFKAGVEPEKSTLAIGTRTLDGIPLPLSAPGS
                     AEGKFAFGGYCVESDEHNVHDLATLDLQNRIVLCLRWTSEGEKDRKLARAMSFQSKWH
                     NLAKRKAAGVIFLYPEGSEPPAPGDFMAGHEAGPIAISLPTKALTELFPWLASAQDKA
                     QKGDVCAAGNECKGTIQIAYTSKQKTGYNVGAFLLPYVKGQRYAILGAHFDHLGRGNF
                     SSMGGRGLIHNGADDNASGTAAVLAAAKSWEEDVRKGTRKLPENTNVLFLNFDAEERG
                     LFGSLAFVNHSPFPLSDAAVMVNLDMVGRYRPEKGMTVQGKDSADPRLLEIFQAGFKE
                     TFPGNAKLKQISGGGGPSDHASFYMKGVPVAFLFTGYHLQYHKPEDDFALLNIDGIDR
                     SSRYAAFIANQVLKLEKPLQFKRASEEGQEGQMEFRLRLGIMPGSYEGGTEGLLVGGV
                     HKGAPVEKTGIQEGDVIISLGGKTIHDVQDLMEFLNTASADQSYTIVFMRNKERITKQ
                     TRLMSE"
     gene            complement(7091..8374)
                     /locus_tag="PKK76_04140"
     CDS             complement(7091..8374)
                     /locus_tag="PKK76_04140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL67685.1"
                     /translation="MVDVRKNKLLLIQSLLLDLIGFSLIFPLVPHLLEYYLNAAANTP
                     MDSWLPPAADYVRGLLPEDRRSSAELIVLIGGILASIYSFLQFSVAPFWGRLSDRIGR
                     RPVLIMTSCGLAASYLLWFFSTSFTMFLLSRVLGGAMAGNMGVVSASMADMTEPKDRT
                     RAMGMLGATFGIGFILGPVIGGLSSLANLPQMFPGLHWLNPYSSCALVSVLMSIGSAT
                     VNSALFTETLLHPVARKESVEIPTSDLRGLPAVLLIGFLFTFLFAAFEFSLTFFYKAE
                     FSLGPAAMGFIFSYLGILIALGQGGLVRRLSGKVQERSMAFVGLSLLPVPLALLAHAP
                     GVGVSLLILIPLSIGASMIRPSLSSLASLLAPGHKQGQSLGVFRSAESLGRALGPICG
                     AYLYWVYGVKFSYATLAALLAVTSLLCLLIRKADA"
     gene            8464..9291
                     /locus_tag="PKK76_04145"
     CDS             8464..9291
                     /locus_tag="PKK76_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67686.1"
                     /translation="MAGSEPIPIADLTAKDHVVSVGKDYIPTIERKLRATIMNIFRDP
                     SGQQGNEISNHELLSEAVFWSLGELINNANKANNRWALLRNALMQRIVAENKLQNDQA
                     AQDKVQTEVDYAIEHNQTDLLKKYNLTNVDLTSSILKLIEMHKTNSFALSERFNKKID
                     VTLRVKAKSGHQTLFVNVINNSPITVIDKQRVEYNLEKIKEDLMMAGKNSRDASVRLY
                     DKQEDHAGGGFGAGLRSIILFLKEGYAPFTAEIKYSTLIQYRSAGNSTIFSVELPIP"
     gene            9384..>9668
                     /locus_tag="PKK76_04150"
     CDS             9384..>9668
                     /locus_tag="PKK76_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNL67687.1"
                     /translation="MREIKTVTVLGANGTMGANSAAIVAAFGHAKVHMLARSVDKAKE
                     GIKIAVDSVRSGVIEKNFIPGTYDADLEKAVSESDWVFELVAEDYAIKEPL"
BASE COUNT         2481 a         2330 c         2538 g         2319 t
ORIGIN      
        1 cggttgcccg cgatcgcaag acagggagcc gccacacctg gagtgtaggc gacggacaaa
       61 tcgtccatgg tttcaatttt catcttgctg gaaacgccga tctttccgcg aagctgctcg
      121 tggatgatca gggatttttg tgaataatcc atatgccatt tcctgaaaat gagcctggag
      181 ataaatggaa attttgtgac cgatttgccg gcattctgac gcgaaccact cgacagggag
      241 ctttccgttt tgagattagg atatgcgcat ttcattcggt ttgaagcttg ctttcgcaac
      301 gtcgatgcta gctttcgtca ccaccagtgc cggcctgggt tacttttaca agataacgcg
      361 acagaacgtc tggaatgaac ttgcagaaag actggcagat atgggccgca ccggccagtt
      421 catattcagc gcagaacaca ggcgtgcgat cgatcggctc aatgaactgt cagagaaaga
      481 ttcttcgcgc aacaaagacc acctgtctat cgggcccggc gaaagcatct ctgtgatctc
      541 cggttcgcga gtgcacgaaa ttgaaaaatc ggatgatttc attctggtgg tccaggctct
      601 cagacagatc aagaatgctt cacgcaaagg gatggtgaat ctcggaattc taggtcagga
      661 gccctggcca aaagacgatc cgccgcgcgt gcgctttacg tacttgctcg ttcccattcc
      721 ggaagtcaaa gacagaagca ttctcaaatt catcgcagac gcagactacg aagcaggaga
      781 tgaaaacggg aacggcaaga ttgatccaga agaagaggcc tcagagattg gaaagctcta
      841 caacgtatcc gctcagccag gtctcatggc tgcattcgat ggcaaggtgc gcacgagtga
      901 ggaatattac gaagatcagt ggggcaaatg gatgtccgcg tatgtcccca ttcttcgagc
      961 ggatggcagc gtcgcggccg tgatggggat tgacatgagt gcaacctcac agttcaactt
     1021 acttgagaaa ctttactata tatgtatcgc ggtcattggt ggaagcgtgg ttcttgcaat
     1081 cattctttct cttgctcttt ctcgtttgat tgcgagcccc attgttgctc ttcgcgccgg
     1141 cgcagaaagg gtgaaagcgc gtgattattc tactcaaatt gtagttaaga gtaaggatga
     1201 gcttggaatt cttgcatcca cgttcaacga catggtccgc gaagtgcgaa cgtatgccac
     1261 tactctggaa gatcgtgtta aagaacgtac ggcggaactc aatgaaagcc gcggtcagat
     1321 gtctgacatt cttcgaaaca ttcaggaagg catcctgacc atagacagat ccggaaaagt
     1381 aaactctgaa tattcaggaa gagttcccga tatgctgggc aagcaaccgg aagggttgga
     1441 atttgcagat ttgttcccgg ctcagttgca gaattctgtt cgtgactttg taggacagct
     1501 cattgaaaat ccattcatgt ccatgcgaat gttcgccgcg gccaatccgc tttctgaggt
     1561 tcgtatcctg tcaggggatc aggagaaggt tctttccttt tcattttcga gagtgaaaaa
     1621 tggtgcagga cagatagaga agctgctcgt tgtcatagat gatcgcacgg aacaaatgaa
     1681 actggagcgc gctcttgcag aacgcgaagc agagcagtct gcgcgcattg aaaagctata
     1741 tcagattctg aatctggacc ccaatgtttt taaatctttt ctggaggaag caggccgaac
     1801 agcggatgtg gtggtcgaga agcttgctct tgcaggatcg gacagatccg ctcttgattt
     1861 gtgttttaga gaagtgcaca ctctcaaggg aaacgctcgt gcactcggac tcgacgcgat
     1921 tgcgaagaat gctcacgaac tggaggatgt gctcgatgca attcgcaaag aaccagaatc
     1981 tccggcgcag gatagaattg catctgcggg ggaacttgga aagaaactca ttgcagagat
     2041 tcaggatggc gggacgttgt ttgacaagat tgtaaacatg aaggacgtgt ttagagtcaa
     2101 gactctcgat gcatctgcag agattgcaga aagatgtgaa agaattgtag agcaggaagc
     2161 actcgccgcg ggcaaagctg ttgcgtttga gtttgagaat cgtgcgggag ttctccgtgg
     2221 agatgctctg gttcgcgttc gcgcgatgct gggtcacatg attcgaaacg ctgtagttca
     2281 tggaattgaa acgcccgacg cgcgaaaaga gaaaggaaaa gcaatcgatg gttccatacg
     2341 ccttctgatg actcacaatg acggccaggt tcgaattgcg gtggaagatg acggggcggg
     2401 cctggatcag gagagaatcg cccgtcacgc gatcgaacag ggaatcttga cgcaagaaca
     2461 ggcgggggtt gcatcacctg cagaggtgtt gagattgatt ttccgtccgg gcttcagcac
     2521 cgcggagcga gttacagaga gtgcgggccg tggagtgggc ctggatgtag tggaagaaga
     2581 agccagagcg ctcggaggca atgttcgagt agagaccaaa agaggaacat tcaccaaatt
     2641 catcgtggtt ctgcccgagt ctgttctgga gaagcgggat tgaattgccg gcataccccg
     2701 gcgtctcgaa tctgacatct gtaatgaaga tacttgtagt ggacgattct gcgatgatgc
     2761 gaagcgtgat taaaagcacg ctggcaagct atggcagcgg taaacagatt gaagtggtgg
     2821 aagcggtcaa cgggcaagaa gccctggaca aactcgcaag cgaaagcatc gatctggttc
     2881 tcctggactg gcagatgcct gtggtagatg gtctttcttt tgtgaagcag gtgcgaggct
     2941 ccgggaaaga cgttccaatc gtcatggtaa cctctgtcac cgacaaagaa aaagtattgg
     3001 aagcagggaa ggccggtgta aacacttaca tcgaaaaacc agttcgaggc agcgtgctct
     3061 gggagaacat tcaggaattt ctgcattaag agggatggaa acgtgaacac ttcgcacata
     3121 ctggctttct ctacatgtct caaaactacg tttcgcgaca tgctttccgt agacatgaca
     3181 caggggccca ttgccgaaca cgtagattat cccgttcgcg cggacgtgag cgcatgcatc
     3241 accatttcat cgaaggcacc cgcgtgcgta gtgcttgcgt ttgacgcaga cagcgccatg
     3301 gatgcggcag agcgtttcac tcagactaaa tctccttttg aagacccgat ggtcggagac
     3361 actgcgcgcg aaattctaaa catcctcgtc ggatccgctc agcgcgaaat ctcagaaaaa
     3421 ttcgaattct cccttcctat ttccattcag ggccgaaacc acgaagtgag ctgctttcgc
     3481 aagggtagaa acattttttg tgacctgcac tggggcacgg gcaagaaagc gactcttttt
     3541 ctgaacctgc cagcactcta aatcacacaa aaaaaacaga tcgaggctgt gaaatcctgc
     3601 gggacagcgc ggaagtgcac cccagtcttg tgattacaaa actgtgtcag gggtttgtca
     3661 tgttcagctt cattccaaag gaagaaaaat acttccggtc cctatcagaa cttgcagcga
     3721 gggtgcgagc gggcagtgaa atcttccttc gcctgtttga cgatttcgga aatcatcaca
     3781 agttcgcaga cgaaatcaaa agcgtagaag ttcagtgcga tacaattgcc gcaagcatta
     3841 catccagact caacacaagt ttcatcactc cgattgatcg cgaagacatc tttctactgg
     3901 tcaaagaagt agacgatatc atggacgcga tcaatggcct ggcgtttcgt tttgatacat
     3961 acagtgttaa gactcctcga tcggaggcag ttgaatttgc aagattgatt caccagggtg
     4021 cagtggagct tgaacacgct ttctccatgc tcgaacaaaa taaaaacctg ggagatcatc
     4081 cgaagaacat caacgacatc gaaaagaaag cagaccatct ataccgcgat gcgatccgca
     4141 gactcttcac agaggaaaaa gatccgatcg aagtcatcaa gtggatgagc atctttgaaa
     4201 ctctcgaaga tgcaaccgac cgctgcaaag atgtggcaga ggcgctcgaa ggcgtcgttg
     4261 taaagaacaa ataaagttta tgaacgcagt tctggttttc gtaatcatat tcgtggtcat
     4321 tgctcttgct tttgactaca tcaatggatt tcacgatgca gcaaattcaa tcgccactgt
     4381 tgtttccacg cgtgtcctga ctcctggtcg cgctgtactg tgggctgctt tttttaattt
     4441 cgtcgccttt gccatttttg gaacacacgt tgccaagaca atcggcggag acatggtgaa
     4501 tattggactc atccaggacg atttcgcgtt tagaaagcta tgggttttgc tctgcggcgt
     4561 gattggagcg attatctgga atttgatcac ctggtattat ggtctgccgt ccagttcttc
     4621 tcatgctttg attggcgggt atgcgggtgc ggcgatcacg gcgaacggcg gcttttccaa
     4681 tgggattctg atttctgcag gctggattaa gacggtttct ttcatcatca tttctcctct
     4741 cgtcgggatc attctgggtt ttctgatcat ggtcgcgatc atgtggatct ttcgcaaatt
     4801 cagtcctgat cgagtggatc gattgtttcg aaaattgcag cttgtgtctg ccgctctctt
     4861 cagcctcgga cacggcggaa acgacgcaca aaaaacaatc ggtatcatcg ccgctgctct
     4921 ggcagcttcc aatctagttc cggatgcagc gaagaatgtt cctttctggg tggttctcat
     4981 atgctacgct gccatagggc ttggaactct atctggcgga tggcggattg ttcataccat
     5041 gggttcacgt ctgaccaagc tcaagccagt gggtggattt gctgcagaaa ctgcaggcgc
     5101 ggctgccatg tttatggcaa cctacggtgg cgttccagtc agcacaaccc acacgatcaa
     5161 tggttccatc attggcgtgg gcgccaccag gcgactttct gccgtgcggt ggggagtggc
     5221 acgcagcgta gtgtgggcct ggattcttac gatccccatg tcaggtggaa tggcagcact
     5281 gctctttaca ctggtccagc tgggctacaa actttctgcc tgacgttatt cgctcatgag
     5341 gcgcgtctgt tttgtgatgc gctctttgtt tctcatgaat acaatcgtat aggattggtc
     5401 cgcggaagca gtattcagga actccattag atcctggaca tcgtggatgg tcttgccccc
     5461 cagggatatg atcacatccc cttcctgaat ccccgtcttc tctacgggtg cacccttgtg
     5521 aactcctccc acaagaagtc cttctgtacc gccttcgtag cttccgggca tgattccaag
     5581 tcggagcctg aattccatct gcccttcctg gccttcttct gacgctcgtt tgaactgaag
     5641 cggtttttcc agtttcagta cttgattcgc gatgaatgcc gcataacggc tggatctatc
     5701 aattccatca atgttcagaa gtgcaaaatc gtcctcgggt ttgtggtatt gcaggtggta
     5761 tccggtgaac agaaatgcaa cagggactcc tttcatatag aaagaagcgt gatcgcttgg
     5821 tccgcctcct ccggagattt gttttagctt cgcgtttcct ggaaaggttt ctttgaaacc
     5881 agcctggaat atttccagca gccgtggatc tgcactgtcc tttccctgca cggtcatccc
     5941 tttctccggt ctgtatctgc ccaccatgtc caggttcacc atcacggccg cgtccgagag
     6001 tgggaaagga gaatggttta caaatgcaag tgatccaaag agaccgcgct cttctgcatc
     6061 aaagttgaga aacaatacgt tcgtattttc tggaagcttt cgcgtcccct ttcgcacatc
     6121 ttcctcccag gattttgccg cggccagcac tgctgccgtg ccggatgcat tgtcatctgc
     6181 gccgttatga atgagtccgc gcccgcccat gctggaaaag tttcctctcc cgaggtgatc
     6241 aaagtgcgct cctagaatgg cataccgctg tcccttaaca tagggaagaa ggaaggcgcc
     6301 gacattatac cctgttttct gtttgctcgt gtatgcgatt tgaatggttc ctttgcattc
     6361 atttccagcc gcgcatacat ctcctttctg agctttgtcc tgcgcagagg caagccacgg
     6421 aaataattct gtgagagctt ttgtaggaag tgagatcgca atcggtcctg cttcatgtcc
     6481 ggccataaaa tcgccggggg cgggcggttc gcttccttct ggatagagaa aaataactcc
     6541 ggcggctttg cgttttgcca ggttgtgcca tttgctctga aagctcatgg cgcgcgcgag
     6601 cttgcgatct ttttcaccct cggaagtcca tctcaggcaa agcacaatcc tattctgcaa
     6661 atcgagcgtt gccagatcat gcacattgtg ttcgtctgat tcaacgcagt atccgccaaa
     6721 tgcgaattta ccttctgcag atcctggtgc ggacaggggc aatggaatgc cgtctaacgt
     6781 acgggttccg atcgccaggg tgcttttttc cggttccact cctgctttga atgaaaactc
     6841 ttgcgtataa ccagatgcga aggcaggata tgcccccatg gcaatgtact gatcgcgaat
     6901 ccagtctgct gtttttttgg cggagggtgt tcctgattct cttccgagca tggaatccga
     6961 gctgagtgtt tctgccacaa agcccgcgta actctgcctg caaaagcctg ttacagatat
     7021 aaggatgagt gcaatgaatc gcatgcttac atggtacggt tggactggcc cgccggcaag
     7081 tgcaatcagc tcatgcgtct gctttgcgga tcaagagaca gagaagcgaa gtaacagcca
     7141 gaagcgccgc cagtgtggcg taagagaatt tcactccgta gacccagtag aggtaggctc
     7201 cacagatagg gccaagtgct cttcccagag attctgcgga acgaaacact ccgaggctct
     7261 gcccctgttt gtggcctggt gcaaggaggc ttgcgagact cgaaagcgaa gggcgaatca
     7321 tggacgcgcc aatcgacaat ggaatgagaa tcaaaagaga tacgccaaca cctggcgcat
     7381 gagcgagaag cgccagaggc acaggaagaa gagacaatcc aacaaaagcc atagatcttt
     7441 cctggacttt ccctgatagc cttcgcacaa gtccaccctg gcccagggcg atcaggattc
     7501 ccagatagga aaaaataaat cccatggccg ccgggccgag agagaattct gctttgtaaa
     7561 aaaacgtaag agaaaactca aaagcagcaa aaaggaatgt gaacaaaaag ccgatcaaga
     7621 gcactgcggg caggccacgc aaatcagacg ttggtatttc cacagattct tttcgcgcta
     7681 caggatggag aagcgtctcc gtaaacagag cagagttcac agtggcagat ccaatcgaca
     7741 tgagcacgga tacaagagcg caggatgaat acggattgag ccagtgaagg cccggaaaca
     7801 tttgcggtag attggcaaga gaagaaagtc cgccgatgac cgggcccagg atgaatccga
     7861 ttccaaacgt cgcacccagc attcccatcg ctctggttct gtcttttggt tctgtcatgt
     7921 ccgccatgga cgcagagacc actcccatgt ttccagccat cgcacctccc aggacacggg
     7981 agaggagaaa catggtgaag ctggtggaga aaaaccagag caaataagac gcggccagcc
     8041 cgcaactcgt catgatcaga acaggcctgc ggccgattct gtctgaaagt ctaccccaga
     8101 aaggagcgac tgagaattga aggaaagaat agatggaagc aaggattccg ccaatcaaga
     8161 cgatgagttc tgcgcttgat cgcctgtctt ctggcaaaag tccgcgcaca tagtccgccg
     8221 ctggaggaag ccagctgtcc atgggtgtat ttgcggccgc attcagataa tattctagaa
     8281 gatgcggaac cagtggaaag atgagggaaa aaccgatcaa atccagcagt agactctgga
     8341 tcaagagtag cttgttcttg cggacatcca ccatggcgac ctttgccggc ggccttacca
     8401 gaaccagaac atttcccaaa aaatgccttt atctatccat gccggatgga aatgtagcgc
     8461 cctatggctg gttctgagcc gattccaata gcggacctca ccgcaaaaga tcatgttgtg
     8521 tccgtcggga aggattatat cccgaccatc gagcgcaagc ttcgcgctac catcatgaac
     8581 atctttcgcg acccttccgg gcaacaggga aacgaaatct cgaaccatga acttctctct
     8641 gaagcggtct tctggtctct aggggaattg atcaacaacg cgaacaaggc aaacaatcgc
     8701 tgggcgctgc ttcgtaacgc gctcatgcaa agaattgtcg cggaaaataa acttcagaat
     8761 gatcaggcag cccaggacaa agtgcagaca gaagtagatt atgcaataga gcacaaccag
     8821 actgacttac taaagaaata caaccttaca aatgtggacc tgacctcttc tattctcaaa
     8881 ctcattgaga tgcacaaaac caattcattc gctctttcag aaagatttaa taagaagata
     8941 gatgtgactc ttcgcgtgaa ggcaaaaagc ggacaccaga ccctgtttgt aaacgtaatc
     9001 aacaattcgc ctatcacggt cattgacaag cagcgtgtgg aatacaacct cgagaagatc
     9061 aaagaagacc tcatgatggc gggtaagaat tcgcgcgacg catctgttcg cctttacgac
     9121 aagcaggaag atcatgcggg cggcggattt ggcgcgggtc tgcgaagcat catccttttc
     9181 ctcaaggagg gatatgctcc ttttacggct gagatcaagt attctacttt gatccagtat
     9241 cgttccgcgg gcaattccac catcttctcc gtagaattgc ccataccctg atccggggtg
     9301 attcgtgcaa aatgcgatcg ttcttgaccg cctacgcgtt tagacggatt caggattatt
     9361 cgaaatagac cctggagttt ggaatgagag agatcaagac tgtaaccgtt ttaggcgcaa
     9421 acggaacgat gggcgcaaat agcgcggcga ttgtggccgc ctttgggcat gcgaaagtcc
     9481 atatgctcgc gcgcagcgta gacaaagcaa aagaaggcat caagatcgca gtagactctg
     9541 ttcgctccgg tgtgatcgaa aagaatttca ttcctggaac gtatgatgcg gacctagaaa
     9601 aggcagtttc tgaaagcgat tgggtgtttg agcttgtagc agaagactac gcgatcaaag
     9661 aacctctc
//
LOCUS       DAORFF010000064         9618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3310_length_9618_cov_3.731465, whole genome shotgun sequence.
ACCESSION   DAORFF010000064 DAORFF010000000
VERSION     DAORFF010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9618
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3310_length_9618_cov_3.731465"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..562
                     /locus_tag="PKK76_04155"
     CDS             173..562
                     /locus_tag="PKK76_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67688.1"
                     /translation="MKRARWLFFLPCLVSLNFVPVEQGFKFPGDWPKIPFYVNPSVPG
                     ERIGGTDALMNAMRDASAMWSSDVTNVRFDFRVSGFSDRAQPFAPAVLDCSSQTTADF
                     AQTAKTVFAFYACGFEEYDFDSKGNHR"
     gene            682..1017
                     /locus_tag="PKK76_04160"
     CDS             682..1017
                     /locus_tag="PKK76_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67689.1"
                     /translation="MIPILRCAHPLGYKVRFSSGAVDCIIPSTSPYIYNQSTTAYAGS
                     TVTGVVTLECRAPTAIKVWSKIATSNPAPGSTATFVVSGNSTLASYSVGDTIFCSDEN
                     LFYHKVTIL"
     gene            1486..1815
                     /locus_tag="PKK76_04165"
     CDS             1486..1815
                     /locus_tag="PKK76_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67690.1"
                     /translation="MGDILFNAASGNNWQRGIARCYTNGSPYQGSDNIVLNVGGNDLL
                     NFYMRKHTYQAEYLFVRYVLNPKGWLKLIFDRGAVDRELEIFWMWSMHLNNFRIALAV
                     ESIGNYI"
     gene            complement(2428..3012)
                     /locus_tag="PKK76_04170"
     CDS             complement(2428..3012)
                     /locus_tag="PKK76_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67691.1"
                     /translation="MKHILFIFAFLSSCASARYIGTQPGEPGSRLTVIAGDVDISFVT
                     FDNVSVSDSTRTIYTEPGSHKASFRYQSRRLYSVQNAEIEFSGNRGDTVMACYTSVIR
                     SGSYPNFDWRWGIFPLVYPGQVDATSLFVKGENPTCLLWSGIGRESIFMQENIDQGAR
                     RDFKNLKGQTLLQFAKSIGAPAENIARLEKNGFN"
     gene            complement(3060..3431)
                     /locus_tag="PKK76_04175"
     CDS             complement(3060..3431)
                     /locus_tag="PKK76_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67692.1"
                     /translation="MTGVAAENAIVVKTHRFSKKEEIPQEPAPAPEVEGIVMNQLESI
                     TVDEGKLLKETFPNLDTRKTVFIAENKMPFMHDSIWVALFCAAVSVGAILLHLSGKKS
                     LAKFEQNQKMKNMGSGPVATG"
     gene            complement(3515..4276)
                     /locus_tag="PKK76_04180"
     CDS             complement(3515..4276)
                     /locus_tag="PKK76_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67693.1"
                     /translation="MLRRMLCQNCGIEAPTREVTYYQNIGALVMRFHKKFQGRVCKRC
                     LHKLFFEYAGISAVAGWWGVISMIVNPFMILIDIFQYLTRLTMPPVPANAKFPQLTQM
                     EIDRLEPETTAIFQRLNTGANLETVVTEIARRENVTPGQVILFLRQKLNQQAQGSAAP
                     APVLPKGPTRPSVLPLPVAAGIVIFCGVIAYAGWNQKALEMSFEPKIAKLSDLAGKSS
                     FENRHVKITDGLALYPLSVFPPGRGHSRGKPQRKV"
     gene            4452..5129
                     /locus_tag="PKK76_04185"
     CDS             4452..5129
                     /locus_tag="PKK76_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNL67694.1"
                     /translation="MPHSALLLESLSPDLLVRIIEEAFIVSITDAKGLIRYVNDTFCK
                     VSQYDRSELIGKPHRIVKSDVHSRDFFKSMWDTIGRGESWHGEICNRKKSGDLYWVDS
                     FILPIAGDTGAPVGYLSIRYEVTQLKEHEQTVQSQESLLIKKNRRLNNLAFWIAHDIR
                     PPIANLIGLSEGMTDALKGGADGSQFAQRVSEEAIRADQILRKIMRTAIASDIVDEQD
                     SEWLSGK"
     gene            5111..5464
                     /locus_tag="PKK76_04190"
     CDS             5111..5464
                     /locus_tag="PKK76_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL67695.1"
                     /translation="MVVWQIDDDPVHTELVEMIIRRTGFSNDTRSFHSARAALKALDQ
                     SLPDLILLDLRMPDMDGWEFIELTRSRKMPPIVIVTSSIDAREKKRALEIPTVRGWIE
                     KPVRVEDIVDLAVDD"
     gene            complement(5467..5874)
                     /locus_tag="PKK76_04195"
     CDS             complement(5467..5874)
                     /locus_tag="PKK76_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67696.1"
                     /translation="MKRFYGIPAFVVMMAIFAGGLSAETRTYRSSQVIGKFVPRMVDE
                     LGNAGFTFTVKTAYNEKRDGKQGFVFLLQPRNTVCELVFFDEAGKSSLVRIRTQDGAD
                     TDRFHKFFTQRLSMTEVGVTSAPQPAPSGWPTP"
     gene            5921..6292
                     /locus_tag="PKK76_04200"
     CDS             5921..6292
                     /locus_tag="PKK76_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67697.1"
                     /translation="MLNRLNMSGPAPFSIVKKPNDLLFFDNLALYYVLQNTPPRVLAR
                     VFLKADSRLSGSLLGILSPKQRETVHFMMTQENDDDAAKNLDAQNGLCLISEGLIARG
                     LIKKQGAHFFGVPGHEKVEPG"
     gene            complement(6340..7446)
                     /locus_tag="PKK76_04205"
     CDS             complement(6340..7446)
                     /locus_tag="PKK76_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HNL67698.1"
                     /translation="MSAPGRICLFGEHQDYLGLPVIAMAVNLRFEIQFEFTDSKTIRI
                     SKPDLGPDVVTDFDPYARQPANSEDFCWGIAQVLIEDGFTFPRGGKATFRSRIPLRAG
                     CSSSSAMSAAWMRLLLEIGEHPEKSKYIDHPETSAYAVYRGEKEKFQGAGGMMDQYSC
                     YLGGLIHVFPDSSRPIPYGVEQLPAKLDGIILIDSGDPKDTQGILSSVGDRARKSIEI
                     AANEMPGFNLLTSNTAEVEGSIPAEKREKTEWKIAVDHLKNRDLCREGLRMFRNTIDP
                     ESLGSMLTEEHRILSGTLGISTPRIDWILDLANKAGAAGGKINGSGGGGTCFCYVPDQ
                     KARMQVLYTLAENNIRHFEVHSDSGARMDQARTF"
     gene            7541..7960
                     /locus_tag="PKK76_04210"
     CDS             7541..7960
                     /locus_tag="PKK76_04210"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HNL67699.1"
                     /translation="MSGKKYILYCDGACRGNPGPASIGAVIYLEGVDEPVLEISKTIG
                     TATNNQAEYRSLIEGLKGLQRYEGHHEVRMDSELVVRQILGQYKVKNEGLKPLFLEAR
                     EIFQGMKCTIKHVPREQNREADRLANEALDNSSHRTN"
     gene            8088..9089
                     /locus_tag="PKK76_04215"
     CDS             8088..9089
                     /locus_tag="PKK76_04215"
                     /inference="COORDINATES: protein motif:HMM:NF022831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL1"
                     /protein_id="HNL67700.1"
                     /translation="MKHAKILAFALAALFATAIQAEGSYVGFGLGIQFNLASLGNTIV
                     NDGLQGTAAGPPPNVRANNGAGGPNCMLTQTCPADAFATDNQKVVVDEKTLVALSRLT
                     LGNMDAKVSGPMIGGELKAFYEKEFASGAWVRGGAGLTRKIAGGSTISHAAGIEWYNV
                     QWNYSRLNIPIMGGFKAGIGETVSVYAGGGLSYNKGFFQLTGTNLGNVPSTFLARYGT
                     SVGLVTTVGAGNQFNINPVTGAVTTPTSTSASLQGIWGSKAKFKAEGLGFNFVTGIEK
                     KLETGDKLFFEIGQVVAGGIGRRGFKDPGAISAFAPVAAYPVDLSGTTYSFGYKMHM"
     gene            9297..>9618
                     /locus_tag="PKK76_04220"
     CDS             9297..>9618
                     /locus_tag="PKK76_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNL67701.1"
                     /translation="MKRLFSLILLVAVHSLSAQTLHMLIVADTADASIGSSTLEDLKH
                     MQNKAREIAANSGMKLNMTVLKAPEFNGTTTLAAVKKLDPGPNDTVFFYYSGHGFRTH
                     NSKTK"
BASE COUNT         2260 a         2373 c         2498 g         2487 t
ORIGIN      
        1 cagcaaacat gaatgccctt cagctcacgg ttccaggcgg tgtcgtgaat ggtttacggc
       61 tgcgcgtgta tggcatgccg cagttttccc agaacgagcg agtgattttg ttcttgaaga
      121 gaattggaaa tgaatacgga attgtggaag gcgaatacgg gaaggtgagc ctatgaaacg
      181 agcgcgatgg ctgttctttc tgccttgtct agtttctctt aattttgtgc ccgtagagca
      241 gggattcaaa ttccctggag actggccaaa gatcccattc tatgtgaatc catctgtgcc
      301 aggagaaagg attggcggta cggatgcgct catgaacgca atgagagatg cttcagcgat
      361 gtggtcgtcg gacgtgacca atgttcgttt tgatttcaga gtttccgggt tttcagatcg
      421 cgctcaacct tttgcgcctg cggtgctgga ttgtagctcc caaacgactg cggattttgc
      481 gcagactgcc aagacagtat ttgcttttta tgcgtgcgga tttgaagagt atgattttga
      541 ttcgaaaggc aatcatagat gaacagaatc gtcgctagta ttttggcgtt attactgagt
      601 tcgtgcaccg cgatcttcgg accgagggat agctgtgacg ctttctgcaa agaggcacaa
      661 gcattttccc agttgcttgt attgattcca atcctgcgct gcgcacatcc actcgggtat
      721 aaagtgcgtt tttcaagcgg cgctgtggac tgcatcattc cctctacatc tccctatatc
      781 tacaatcaaa gcactacggc atatgccgga tccacagtga ctggcgtggt tactctggag
      841 tgtcgcgcac caactgccat caaggtctgg agcaagatcg cgacttcgaa ccccgcgccg
      901 ggatctacgg ctacattcgt tgtgtctgga aattcaacgc tcgcgagtta ctcggtgggc
      961 gacacgatct tctgctccga cgaaaacctt ttctatcaca aggtaaccat tctatgaatc
     1021 caattcgtca ggtttcttat ctgggttgcg cgtcgctctg tccctctggt caaatgcgct
     1081 atttccgcag gacccgggcg ggattggaca ggcaccatca tgcgcggact atccctttgg
     1141 acgcaacata actgttaccg ggcacagccg cgcctacgag gttgcggtgt tcactttgcc
     1201 agccgactat ggaagtgcca ttggggacgc aaggctccgc gatgacgcca ctgacgcgac
     1261 caatgtgaag atttttgctc cgggagcagt aaccggcgtt cagaatcttg cctggcccgg
     1321 agcgcaggcg acggattcag cgccagcaca ttgggtcatg ggcttgatca tatctatctt
     1381 tgattcgtat cttcctaaag ataacttctg gagcgttgtg gttcatttgc ccgaattctt
     1441 gagaaacccg atggttcatc taaatttgca acaaacagtt ttggtgtggg ggatatcctg
     1501 ttcaatgccg catccgggaa caactggcaa aggggaatcg cgcgctgcta cacaaacggt
     1561 tctccatatc aaggttcaga taacatcgta ttgaatgtcg gcggtaacga tttgttgaat
     1621 ttctacatgc gcaagcatac gtaccaagcg gagtaccttt tcgtgcgtta cgtcttgaat
     1681 ccgaagggct ggctcaagct tattttcgat cgtggcgctg tagacagaga gcttgagatt
     1741 ttttggatgt ggagcatgca cctgaacaat ttcagaatag cactcgccgt cgaatccata
     1801 gggaattaca tctgagcgac tttcacggac ccggctggga tcatgtgggg cgctattcta
     1861 agcttctgct ggttgaggaa gcgccttccc tggcttcatg ggtggtcaat ttgtttcccg
     1921 tccctggcgg cggagcgtca gacgatccac cgtggcgtat cattttatat tttgcggacc
     1981 cgtagccatc ccggcatttg tgaacgcgag gaaacggaag aatttcggct gcgcgacagt
     2041 cagttgcggc ttttgttgaa tgatcgtcag aaagaagact gtcactgagg gcggatcgaa
     2101 gctcagtcag ggtgcgcgcg accacctgta agtctgcccg cccgaaacgc gctgcaaaca
     2161 agctacgtcg tgcgctgcgt cccggcggaa gaatcgtaac gaactacctg gcccgcccac
     2221 cgcggcgcaa cttcggatga ctacggagta tttgcagagc aatttgcggc attgggacgc
     2281 gctgcaggct tccagaatgt gcaagtgtac actctgccca tgcgtccaat cccctgttgc
     2341 acaattgttt ttgaatagcg gctgccttgc aacggagttg cgcccctgtc gcctgatcgc
     2401 tcccagggat gagctttcgc ttaacaatta gttaaagcca tttttctcaa ggcgggcaat
     2461 gttttccgca ggtgcgccga tgctttttgc aaactgaagt agtgtttgcc ctttcaaatt
     2521 cttgaaatcc cggcgggctc cctggtcgat gttctcctgc ataaagatgc tttcgcgccc
     2581 gattccactc cagaggagac aggttggatt ctctccctta acaaataggc ttgttgcgtc
     2641 aacctgaccc ggatatacaa gcgggaagat tccccagcgc cagtcaaaat taggatagct
     2701 gcccgacctg atgacggagg tatagcacgc catcacagtg tcgccgcgat ttccggagaa
     2761 ttctatttct gcattttgaa ccgaatacaa gcgacgactc tggtagcgaa agctagcctt
     2821 gtggctccct ggctcagtgt agattgttct cgtggaatcg ctcaccgata cgttgtcgaa
     2881 ggtgacaaat gaaatgtcaa cgtctccggc gatgacggtt agcctggaac ctggttctcc
     2941 gggctgggtt ccaatatacc gagcggaagc gcacgagctc agaaaggcga aaataaacaa
     3001 aatatgtttc acatgttcct ctgatatggc gcatttgcgc aatgccgcat aacgctacac
     3061 tagcccgtcg ccaccggccc ggaacccatg ttcttcattt tctggttttg ctcgaacttt
     3121 gccaggctct tttttccgct tagatgtaga aggatcgcac caacggaaac ggcagcgcag
     3181 aacaacgcga cccatatgga gtcgtgcatg aatggcattt tgttttccgc aatgaagact
     3241 gttttgcgtg tgtccaggtt cgggaaagtt tctttgagca acttcccttc atccacagtg
     3301 atcgattcga gttgattcat cacaatccct tccacctcag gagcaggggc cggttcttgc
     3361 gggatttctt ccttttttga gaagcgatgc gtcttgacga cgattgcatt ttcagcagcc
     3421 actccggtca tggctggccc ggccgctgtc tgggtgattt gaaagttctg gagaacggat
     3481 ctgtcattgg ggaccagtaa gtagtacgta taattcagac cttcctctgt ggttttcccc
     3541 tgctgtgtcc tcttccaggt ggaaagacgg aaagtggata gagagcaagg ccatccgtga
     3601 tcttgacgtg gcggttttca aaactgctct tgcctgccag gtctgagagc tttgcgatct
     3661 ttggttcaaa gctcatttcg agagcttttt gattccaacc ggcataggcg atgacaccgc
     3721 aaaagattac aattcctgcc gcaactggaa gtggcaatac agaagggcgc gtaggtcctt
     3781 tgggaagaac gggtgccggg gctgcgctac cctgggcttg ttggtttagt ttctgtctga
     3841 gaaatagaat cacctgaccg ggcgttacat tctctctgcg cgcaatctct gtcaccactg
     3901 tctcaaggtt tgctccggta ttcagtcttt gaaagatggc cgttgtttct ggttcgagtc
     3961 tgtctatttc catttgagtg agctgcggaa atttcgcgtt cgcaggaacc ggaggcatgg
     4021 tgaggcgagt cagatactga aagatgtcaa tgaggatcat gaatggattt acgatcatgg
     4081 agatcacacc ccaccagccg gcgactgccg atatcccggc gtattcgaaa aagagtttgt
     4141 gcaaacaacg tttgcaaact cttccctgaa acttcttatg aaagcgcatt acgagtgcgc
     4201 caatgttctg gtagtacgtt acttcccttg tgggggcttc aattccgcaa ttctggcata
     4261 gcatgcgccg aagcacagag tacgaaactc attcgtaaat cactttttgc aaaagcactt
     4321 tttctgtctc atggggcggg tgattttgag acgatctgtt tgctttgtgc cgttcgttac
     4381 cagaaatgtg acgttcattc acccccggaa tattttgttt gcggattcta aaccagagga
     4441 aattttgcgc cgtgccccac tcagcgctat tacttgagtc cctttcgcca gaccttctgg
     4501 tgcgaatcat tgaggaggcg ttcattgttt ccatcacaga tgcgaaggga ctgattcgat
     4561 acgtaaatga tacattttgt aaagtatctc aatatgacag atctgaactg atcgggaagc
     4621 cgcacagaat cgtgaagtca gatgttcatt cgcgggactt cttcaaatcc atgtgggaca
     4681 cgattggtcg cggagaatcc tggcatggag agatctgcaa tcgcaagaaa agcggagatt
     4741 tatactgggt ggatagtttc attctgccca tcgccggaga cacaggagct cccgttggat
     4801 acctctcgat tcgatatgaa gtcacgcagc taaaagagca tgagcagaca gtccaaagtc
     4861 aggaatctct gttgatcaag aaaaacagac gattgaataa ccttgcattc tggatcgctc
     4921 acgacattcg gccgcccatc gcgaacttga ttggtctttc ggagggcatg acagatgctt
     4981 tgaagggcgg cgcagatgga tcgcaatttg cgcagcgcgt tagcgaggag gcaatacgag
     5041 cggatcaaat cctcaggaag atcatgcgca cggccatcgc aagtgacatt gtggatgagc
     5101 aggattcgga atggttgtct ggcaaataga cgacgatccg gttcacactg agcttgttga
     5161 aatgatcatc cggcgaaccg gattttcaaa tgacactcgg tcctttcatt cagccagggc
     5221 ggcgctcaaa gcccttgatc agtctttgcc ggatctgatc ctcctcgact tgcgaatgcc
     5281 ggatatggac ggctgggagt tcattgaact caccagatct cgcaagatgc cgcctatcgt
     5341 tattgttact tcttctattg atgcgcgaga gaaaaaacgc gcgctggaaa taccgaccgt
     5401 gcgcggatgg attgagaaac cagtccgcgt agaagacatt gtagatctgg cagtggacga
     5461 ctgatctcag ggcgtaggcc agccagaagg tgctggctgc ggagcggatg taactcccac
     5521 ttcagtcatg ctaagccgtt gggtaaaaaa cttatgaaag cgatctgtat ccgcgccgtc
     5581 ttgtgtgcga atgcgaacca gagatgactt tccggcttca tcaaagaaga ccagctcgca
     5641 gactgtgttg cgtggttgca gtagaaaaac aaagccctgc ttgccgtccc tcttttcatt
     5701 gtatgctgtt ttcaccgtga aagtgaatcc tgcattgcca agttcgtcca ccatgcgcgg
     5761 aacaaatttg ccaatcacct gcgaggaccg atacgtgcgc gtttctgctg acaggccgcc
     5821 tgcaaagatc gccatcatta ccacaaatgc tggaattccg tagaaacgtt tcatctcact
     5881 ctccagttcg gcaaaaaaag cattttctta accgttgtcc gtgctaaatc ggctaaatat
     5941 gtcaggaccg gcgccctttt ctatagtaaa gaagccaaac gacctcttgt tctttgacaa
     6001 tcttgcgttg tactatgttc tacagaatac tccaccaaga gttctggccc gcgtttttct
     6061 caaagcggat tcgaggcttt cgggaagtct gcttggcatt cttagcccaa aacagcgcga
     6121 aactgtacac ttcatgatga ctcaggaaaa tgatgacgac gcagccaaga atctggatgc
     6181 gcagaatggt ctttgtttga tttcagaagg tctcatcgcg cgcgggttga tcaagaaaca
     6241 gggcgcgcac ttctttggag tgcccggtca cgaaaaagta gagccgggct gaccgagcga
     6301 gtgccagatc gtgactgcta cttttctctt tcgcctcttc tagaacgttc ttgcctgatc
     6361 catgcgtgcg cctgaatcac tgtgcacttc aaaatgccgg atgttgtttt cagcaagtgt
     6421 gtagagaact tgcatgcgcg ccttctgatc cggaacatag cagaaacagg ttccgcctcc
     6481 acctgaaccg ttgatttttc cgcctgcagc ccctgctttg tttgcaagat ccagaatcca
     6541 atctatgcgc ggcgtggata ttcctagagt cccggacaga attcggtgtt cctccgtgag
     6601 catcgagccc aggctttccg gatcaattgt atttctaaac atccgcagtc cttctctaca
     6661 cagatcgcga ttcttgaggt gatcgactgc aatcttccat tcagttttct cgcgcttttc
     6721 tgcggggatg gatccttcaa cttctgccgt gttcgaagtc agcagattga atccgggcat
     6781 ttcattggcg gcaatttcga ttgattttcg cgcgcggtct ccgacggagc tgagaattcc
     6841 ttgcgtatct ttaggatcgc ctgagtcgat cagaatgatt ccgtcgagct ttgccggcaa
     6901 ttgttccacg ccgtaaggga tgggtcgaga cgagtccggg aagacgtgga tgaggccgcc
     6961 caggtagcag gagtattgat ccatcattcc acctgcgccc tggaactttt ctttctctcc
     7021 gcgatagact gcataggctg atgtttccgg atgatcgatg tatttgcttt tttccggatg
     7081 ttcgccgatt tccagtaaca atcgcatcca tgcggcagac atcgcggaag aagaagaaca
     7141 gcccgcgcgc agtggaatgc gcgaacgaaa cgttgcctta cctccgcgcg gaaatgtaaa
     7201 gccatcttca atcagaacct gtgcgattcc ccagcagaag tcttccgagt ttgccggttg
     7261 cctggcataa ggatcaaagt ccgtgaccac gtcgggtcca agatctggtt ttgatattcg
     7321 tatggttttg gaatcagtga attcaaactg aatctcaaag cgaaggttaa ccgccatggc
     7381 aatgacgggc agtcccagat aatcctgatg ttctccgaat agacagatac gcccaggcgc
     7441 gctcacaaac agcatgcctc gtgatgcagg aacgcttgaa acggacaatt ctaattctat
     7501 tgcttgacgc gctatcgcac aggagtcgta tcttcaggtc ttgtccggta agaaatatat
     7561 tctctattgc gacggagctt gcaggggcaa tccagggcct gcgtccattg gcgccgtcat
     7621 ctacctggaa ggggtggacg agcctgtgct tgagatttcc aaaacaatcg gcacggccac
     7681 aaacaaccag gcagaatacc gttcattgat cgagggtttg aagggcttgc agagatacga
     7741 aggccaccat gaggtccgca tggactcaga gctggtggtt cgccagattc ttgggcaata
     7801 caaagtcaaa aatgagggcc ttaagcctct ttttctggaa gcccgcgaga tttttcaggg
     7861 gatgaagtgc accatcaagc atgtgccccg ggagcaaaac cgggaagcgg atcgtctggc
     7921 aaacgaggcg ctggacaact cctcgcatag gacgaattag accgattctg cccggccttc
     7981 gttccctttt tttttatttg acaaccacct ttttggccgc tttttcaagt ctgcgctggc
     8041 ggagggggaa tgcacttgtc cgtcgtgcaa aaatttagga gtaaaagatg aaacatgcga
     8101 agatacttgc cttcgcgctg gcggccctct ttgcgacggc gatccaggca gaaggcagtt
     8161 acgttggctt tggtctgggg attcaattca acctggccag cctcgggaac actattgtaa
     8221 atgacggtct gcaaggtact gctgctggcc caccaccaaa cgttcgcgcg aacaacggtg
     8281 cgggcggccc aaactgtatg ctcactcaaa cttgtcctgc agatgcattt gcaactgaca
     8341 accagaaggt agtcgtcgac gaaaagactc tcgtagctct ttccagactt actcttggta
     8401 acatggacgc gaaagttagc ggtccaatga ttggtgggga actcaaagct ttctatgaga
     8461 aagaatttgc aagcggcgcg tgggtgcgcg gtggagcagg tctcactcgc aagatcgcgg
     8521 gtggtagcac aatttctcac gcagctggta ttgaatggta caacgttcag tggaactaca
     8581 gccgtttgaa catccctatc atgggcggtt tcaaagccgg aatcggcgaa actgtatctg
     8641 tatacgctgg cggcggtctg agctacaaca aaggcttctt ccaattgact ggaacaaacc
     8701 tcggtaacgt accaagtacg ttcctcgctc gttacggaac ttctgttggt ctggtaacta
     8761 ctgtgggcgc gggcaaccag ttcaacatca accctgttac aggtgcagtg actactccta
     8821 caagtacatc tgcgagcctc cagggtatct ggggatcaaa agcgaaattt aaggcagaag
     8881 gccttggatt taactttgta actggtattg aaaagaaact ggaaactggc gacaaactgt
     8941 tcttcgaaat tggacaggta gttgctggcg gcatcggtcg tcgcggcttc aaagatccag
     9001 gtgcgatcag cgcatttgct ccagttgcag cttaccctgt tgacctttcc ggaacaacct
     9061 attcattcgg atacaaaatg cacatgtaat tgtgtttaaa ctttggggaa aagccgcgct
     9121 ctcgagtgcg gctttttttt tgcgcgatat ttgaaactgt ccggttcatt tgacccgggt
     9181 gaaaaatgca tcattgtcgc tttacgcgaa aatgatctga tcaggttcgc aagatgaacc
     9241 tttgagttct tctttacgcc taagaataaa ggtgctgaaa tgtttgcggg gtgtttatga
     9301 agcgtttatt ctctctgatt cttctggtcg cggtgcacag cctgtccgct caaactctgc
     9361 atatgctcat cgtggcagac actgcggatg cgagcatcgg gagttccact ctcgaagatc
     9421 taaagcacat gcaaaacaag gcgcgcgaga tcgctgcgaa ttctggcatg aagctcaaca
     9481 tgaccgtttt aaaagcaccc gaatttaatg gtacaaccac tctggccgct gtgaagaagc
     9541 tcgatccagg cccaaatgat accgtcttct tctattactc cggccatgga tttcgcacgc
     9601 acaattcgaa aaccaaat
//
LOCUS       DAORFF010000065         9590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3327_length_9590_cov_3.916728, whole genome shotgun sequence.
ACCESSION   DAORFF010000065 DAORFF010000000
VERSION     DAORFF010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9590
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3327_length_9590_cov_3.916728"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PKK76_04225"
     CDS             <1..303
                     /locus_tag="PKK76_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67702.1"
                     /translation="TSAQGSLVGTATAPYMPNGTSPTFSHVGAVASTAQTSIVTQRVS
                     YQVAPPATKQFQYCELYYFRLTALTRAGIESAPSAQASSCAAINTALCPSGTPCRP"
     gene            307..978
                     /gene="rsmI"
                     /locus_tag="PKK76_04230"
     CDS             307..978
                     /gene="rsmI"
                     /locus_tag="PKK76_04230"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNL67703.1"
                     /translation="MPTLSIVSTPIGNLEDVTHRAIRILKESDLILCEDTRKTSILCR
                     HYGIDRPLKSYRIHRLDEDGAYAIDKLREGWSLSLCTDAGTPALSDPGTHLVRLVRAT
                     LPDVSIVPIPGPSALTAALSVCGFRPNPFIFAGFLSIKSGARKRFFEQYRDFDGLIIF
                     FESVHRITKVLAEVREVLPERPVFVGREMTKVYEEYRIVLPEDRITEKGEFTVILGLP
                     GKGEA"
     gene            1088..2020
                     /locus_tag="PKK76_04235"
     CDS             1088..2020
                     /locus_tag="PKK76_04235"
                     /inference="COORDINATES: protein motif:HMM:NF021466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="App1 family protein"
                     /protein_id="HNL67704.1"
                     /translation="MRDPSGGWNVTIHGNVHNPSTDSTLRKILVDLIRPGDLDSPLEK
                     ENYEKRVRLFLVDNLGWKLVRLRIAGRQVEMSVTGSNGHFYGSVHLSEEEVGPFRFRG
                     VIPYESQVADRVFRGESVIISDAGPIIVSDIDDTIKISDARDKKELLRNTFMRVFRAT
                     PGMPDFYKLLQSRGSSFYFVSASPWQLYPELAQFARPIYPPGVFRMKNFRLKDSDFMS
                     LFKDPYAYKIETIEPIVRSFPGRQFVLIGDSGEKDPEAYVEIARRYPASVKKIWIRMA
                     YGEEPERISKLKATIAPDTLFTFLDPSTLDSAAL"
     gene            complement(2017..3282)
                     /locus_tag="PKK76_04240"
     CDS             complement(2017..3282)
                     /locus_tag="PKK76_04240"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL67705.1"
                     /translation="MKTQSQMGLRESLRNRFRSARSLDPAILRILDDESLRNAKALNS
                     FRVFGAAVWVLLAMVLGYWKNQSDWIIQIPWTVAYLASAVLVLLFAGKSLTVTRWSIV
                     FLDFPLIFVAMKLSIPTAPHPEVAAALTTAIYLLLIMPAPSGVHHAPTIVASIEAAIF
                     SGIVLNDAGVPFPMWTGSVCLILGLGCFLAVNVQRRVLTVANQYAAEQTRRHELARYF
                     SPAVAEKIVSTGKEAGGHEKREITILFSDIRGFTALSETLESEEVISFLNEYLTIMVH
                     ILFKHGGTLDKFIGDGILAYFGAPMEQSDHARRAVLCAQDMLRAMTELNSVRAVRSET
                     PLEIGIGIHTGKVVLGDIGSEQRREFTIIGDSVNLTARIESITRDLGHALLVTSAVTE
                     RAGGGFSWEAAPPVQVKGKREPVQTYFLA"
     gene            complement(3294..3860)
                     /locus_tag="PKK76_04245"
     CDS             complement(3294..3860)
                     /locus_tag="PKK76_04245"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNL67706.1"
                     /translation="MPDESMPRSVFLKKAGTTFLLLTTSGTVLSTLHCGTHMARDLRF
                     KNLDDALLEIDRVSVSAKVETNGPWNSSQVFAHLAQSIEYSMTGYPENKPAIFRKTIG
                     RIVLAKFLGQGYMQHNLSDAIPGAPALDANLPFPDAVARLKKAISDFRAFQDHLAMHF
                     VYDEATKDQYERVHAYHIANHLSAFTIG"
     gene            complement(3913..4407)
                     /locus_tag="PKK76_04250"
     CDS             complement(3913..4407)
                     /locus_tag="PKK76_04250"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNL67707.1"
                     /translation="MMLLVDAFNVIYKFPVLEEHMAQGRLAEARRGLLEIVYDFAKKW
                     KKPLDIHLVFDGKKNKGDATESEIVSGIKVIYSHDLSADHIIKQFIKHNARPADIRVV
                     TSDKDILGHAKKWHCDRMTSEDFHKWVVSTLEDKPLPEEKPQTEDVDVGYWMSMFKNR
                     RPGK"
     gene            4455..4877
                     /locus_tag="PKK76_04255"
     CDS             4455..4877
                     /locus_tag="PKK76_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNL67708.1"
                     /translation="MILWLKAFHIIGFTAWFAGIFYIWRLFVYHAETTSEDVRNQLAI
                     MEYKLYKYIMRPAMVVTLFFGFALLYLQWEAFFRSAWIWLKLCLVALVLANHFMAEAY
                     RKKLARGMAYKSRIFRILNEMPTVLLIGIVLLVVLKPF"
     gene            4916..6568
                     /locus_tag="PKK76_04260"
     CDS             4916..6568
                     /locus_tag="PKK76_04260"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNL67709.1"
                     /translation="MKFSELHIHPDIIRACTDAGFETLTEIQEKCLIPATQGRDIAGI
                     SQTGTGKTVAFLLPLLDRIVKDNLPGPAALIITPTRELCLQIAEEALKLTKHGPVSVS
                     AVYGGEGYRKQEEELAKNPQIIVATPGRLIDYIKQGKIDLNKLHFLVLDEADRMFDMG
                     FIRDIGFIMKKVPREAQTMLFSATLSYYVMRLAADFMNDPVEVRIEAETVAVDKIDQQ
                     LFHLGKDEKMPYLINQVLAVPGIRAIVFTNLRVYVSTIVNNLRRYGVHATGLSSMLDQ
                     KKRVRLLKDFKLGKYSVLVATDVASRGLDVDDITHVFNYDLPQDAESYVHRIGRTARA
                     GRSGISISYCSEMDYEALPRVESYLGKKIPVKTVDVELLKFPAGQFTPFQEAHGERVE
                     GEDRSDRDSNRDGRGGGRNRGGRKRKGQDRPGFAPARGRDRNDQDPSKEGGARAGVNS
                     DRNQKDDRGRRDDRGRRDSRDRRDDRGRRDGHPGQDRGRNDRHGQGNRGRDGGRPGRG
                     DGRHGSHRHRDRDRTRRESVPQKKPGIIARILSIFKKKKPEA"
     gene            complement(6593..7267)
                     /locus_tag="PKK76_04265"
     CDS             complement(6593..7267)
                     /locus_tag="PKK76_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67710.1"
                     /translation="MSQCRLTFLKTGLSFVFLFFTVGLYADPPEWRKDMPWFDNPKED
                     YRAYGPDDIKPYLEKPKERSRSGFALPDLTQLVYAVAAILAGAAIYLIVRRILERRPA
                     EEEVLEETPEMKVSVVVDLPEAHLPDQDLVKVIEQLLAAGNFRRAGMLLFQLACNRLK
                     AEGLLDFHEYQTPREIASTLNTEREGEILAQAGKVFEDCAYRPEEPSGAAVISLKERV
                     FSLGPA"
     gene            complement(7270..8094)
                     /locus_tag="PKK76_04270"
     CDS             complement(7270..8094)
                     /locus_tag="PKK76_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67711.1"
                     /translation="MNLLDLRIPLRRRRLDEIFDLAVIVLKSNHWRYGRILFPFTISC
                     IAINYGVTKLVKEDVLANGMIVLLLLFESSVLQMIFNALNGRIVFELKPRFRDVLADT
                     KQTAPVYIGRMAILNTLYYTLLFPFIIPPVRALFTHFFMGEVIILERLRGAPLGLRAA
                     ALARGQGDRIVGFFVFSCLMFLAGLVSTVYGLSSMRELMGGWAEKNIPSPGFAPTGVV
                     FQLVFFPSIAYFCLVRFLLYLDTRIAGEGWDIELLLARGIQETQQEPEARYERRSA"
     gene            complement(8091..9038)
                     /locus_tag="PKK76_04275"
     CDS             complement(8091..9038)
                     /locus_tag="PKK76_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNL67712.1"
                     /translation="MTPAIFIEQRSKDWDDLEKMLPDLERKFRLPVREILKFARLYRA
                     VSTDLSVAQAFRLSRMQQARLEGLVSRGHSVLYSIPRRKLTDVRRYIFETVPAAIRKD
                     VYVRICLLVFYVPFFLCAGMAYVSRPFAAQVVGEAVLEEYHDMHEGERGEIKLGDLVG
                     ASGFYIANNIGLNLITFGLGALVGIGSLFFTLYNAVFLGTIIGYLMSTPARDHIVSWA
                     AAHAPFELTAIGLSAAAGVRIGFCLIAPGARSRLLAIREESLTAMPALYSAAMLTFFA
                     AFIEAFLGPWNPGVNVKLALGGVTTLLTLAYFLAGGRGK"
     gene            complement(9048..>9590)
                     /locus_tag="PKK76_04280"
     CDS             complement(9048..>9590)
                     /locus_tag="PKK76_04280"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNL67713.1"
                     /translation="QWFYFFAFEWLNKGRTPGKMALGLRVISIDGTAVDVVQILIRNM
                     LRIADMFPFKFLVWLLYLPTYTIGLISSVMSAPRFRRLGDLAAGTIVIREIPTEKEKA
                     ITVRVPEDLVRKISFRILPSATLVQALHDFASRYEKMHPARRMEIADRVRPGLEWLAG
                     HQECTSEQLLLACHRKLTED"
BASE COUNT         2356 a         2394 c         2398 g         2442 t
ORIGIN      
        1 acttccgctc agggtagcct tgtaggaact gcgactgctc cttacatgcc aaacggaaca
       61 tcgcctactt tctctcatgt aggagctgtt gccagcaccg cgcagacaag catcgtaaca
      121 cagagggttt cctatcaggt ggctcctcct gccacaaagc agtttcagta ttgtgagctt
      181 tactacttcc gcctgaccgc tcttactcga gccgggattg aatctgctcc ttctgcccag
      241 gcatcttcct gtgcggccat caatacagcg ctatgtccat cgggcactcc ctgccggccc
      301 tgattcatgc ccactctctc tatcgtaagc acacccatcg gcaatctgga agacgtaact
      361 catcgtgcca ttcgcatact caaagagtct gatctgattc tttgcgaaga tacgcgaaag
      421 acatcgattc tgtgccgcca ttacggcatt gaccgacctc tgaaatccta tcgcattcac
      481 aggctggatg aagacggcgc ttatgcaatc gacaagctgc gcgaaggctg gtctttgtct
      541 ctctgcacgg acgcaggtac tcctgcactc tctgatcctg gcactcatct cgtgcgtctc
      601 gtgcgcgcaa cccttccaga cgtttccatt gtgccgattc ccgggccttc tgctctgacc
      661 gctgcccttt ctgtttgcgg ctttcgtcct aatccattca tcttcgccgg tttcttgagc
      721 atcaagtccg gcgcgcgaaa gagattcttt gagcagtaca gagatttcga tggtctgatc
      781 atattctttg aatcagttca caggatcaca aaagtgttag cagaagtgcg agaggtattg
      841 ccggagaggc ctgtttttgt aggcagggaa atgacaaagg tctatgagga atatagaatt
      901 gttctccctg aagatcgcat aacggaaaaa ggagagttta cagtcatcct tggtttgcca
      961 ggaaaagggg aggcttagta tttcacgcgc tctgattctc agtcttcttg tctgtcttca
     1021 ttgcagcact tttcttgttt cgcctgtgga cgcgggcgaa gagatcgttt tttttccaac
     1081 agaagctttg cgtgatccat ctggcggatg gaacgtgacg atacacggca acgtgcacaa
     1141 tccgagtaca gattctactc tgcgaaagat cctggtagat ctgattcgtc ccggcgatct
     1201 cgatagtcct ctggaaaaag aaaactacga aaaaagagtg cgcctctttc tagtggataa
     1261 ccttggctgg aagcttgtgc gccttcgcat agcaggccgt caggtggaaa tgagtgttac
     1321 ggggtcaaac ggtcattttt acggttctgt tcatctttct gaggaagaag taggcccctt
     1381 tcggttcaga ggcgtcattc cctacgaatc gcaggtggca gatcgtgtgt ttcgcggaga
     1441 atcagtgatt atttcggatg ccggccccat catcgtcagc gacattgatg acacgattaa
     1501 gatcagtgat gctcgtgaca aaaaggaatt gctgagaaac acgttcatgc gcgtgtttcg
     1561 cgccactcct ggaatgcctg acttctataa attgcttcaa tccagggggt cttctttcta
     1621 ttttgtgagc gccagtccat ggcagttgta tccggaactg gcacaatttg cgcgccccat
     1681 ctatccgccc ggggttttcc gcatgaagaa ctttcgtttg aaagattctg atttcatgag
     1741 cttattcaag gacccatacg cttataaaat tgaaaccatc gaaccgattg ttcgttcctt
     1801 cccgggaagg cagtttgttt tgatcgggga ttccggagaa aaagatccag aagcgtatgt
     1861 ggaaattgcg agacgttatc cggcatccgt gaaaaagatc tggatccgaa tggcttatgg
     1921 ggaagagcca gagagaatct ctaagctcaa ggcgacgatt gcaccggata ctctgtttac
     1981 gtttcttgac ccgtccactc ttgattccgc cgcactctaa gccagaaagt aagtctggac
     2041 gggttctctc tttcctttca cctgaacggg tggggcggct tcccaggaaa atccacctcc
     2101 ggcgcgttcc gtgaccgcgg atgtaaccag tagagcatgc cccagatctc gcgtgatcga
     2161 ttcaatgcgt gcagtcaaat tcactgaatc tccaatgatt gtgaactctc tcctttgttc
     2221 agacccaatg tcccccagga caaccttgcc tgtgtggatt ccaattccga tttcgagagg
     2281 tgtttcgctt cgcactgcgc gaacagagtt gagttcagtc atggcgcgca gcatgtcctg
     2341 cgcacagaga actgcgcgac gagcgtgatc tgactgttcc atgggtgctc caaagtatgc
     2401 aaggattcca tctccgatga atttatccag cgtgcctccg tgcttgaata gtatatgaac
     2461 cataatagtt aagtattcat ttagaaagga aatgacttct tcgctttcca gtgtttctga
     2521 cagagctgta aacccgcgaa tgtctgagaa tagtatggtg atttctctct tttcgtgtcc
     2581 acctgcttcc ttgcctgtgc ttacgatctt ttcagcaaca gcgggagaga aatagcgagc
     2641 cagttcatgc cttctggttt gttccgccgc gtattgattg gcaacagtga gaactctcct
     2701 ctgtacattt actgcaagga aacacccaag gcctagaatc aaacagacag atccagtcca
     2761 cattggaaaa ggaacgcccg catcattgag cacgattccc gaaaaaatgg cggcctctat
     2821 gcttgcgacg atggtgggtg cgtggtgaac tccggaaggc gcgggcataa tgagaagcag
     2881 atagatcgca gtcgtgagag cggctgccac ttctggatga ggtgccgttg gaatagatag
     2941 tttcatggca acaaagatga gcgggaaatc caaaaacaca atggaccacc tggtcaccgt
     3001 gagactcttg cctgcgaaaa gtagaaccag aacagcgctc gcgaggtatg ccacggtcca
     3061 cggaatctgg atgatccaat cgctttgatt tttccagtat cccagaacca tagccagcag
     3121 tacccacacg gcggctccga aaacgcgaaa gctgttgagt gcctttgcat ttcgcaggct
     3181 ttcgtcgtct agaatgcgaa ggatggccgg atcaagagaa cgcgcgctgc gaaacctgtt
     3241 tcgcaaggat tctcgcagtc ccatctgcga ttgagttttc aaaatctgtt ccgtcagcca
     3301 atcgtaaacg cggacaagtg attggcgata tggtaggcgt gcacgcgttc gtactggtct
     3361 ttggttgcct catcatagac aaaatgcatg gcaagatgat cctggaatgc tctgaaatca
     3421 gagattgctt tcttgaggcg cgcgaccgca tctgggaaag gtagattcgc atcgagagcc
     3481 ggagcgccag gaatggcatc gctcaaattg tgctgcatgt aaccctgtcc aaggaacttc
     3541 gcaagtacaa tgcgaccaat ggtcttgcga aagatggcgg gcttgttttc agggtaccct
     3601 gtcatcgaat actcgatgct ctgcgccagg tgtgcgaaga cctgggagga attccagggc
     3661 ccgtttgttt caaccttcgc ggacacgctt actctatcaa tttccaggag tgcgtcgtcc
     3721 aggtttttga atcgtagatc ccgagccata tgtgttccac agtgaagagt cgaaagaaca
     3781 gtgccagacg tagtgagtaa caggaaagtg gttcccgctt tcttcaagaa taccgaacga
     3841 ggcatggatt catctggcat ggggttcctc cgcgcaccag aatgacaccg cgtgtttgat
     3901 cgtgcaaccc aatcacttgc ccgggcgtcg gtttttgaac atggacatcc agtatcctac
     3961 atccacatct tcggtctgtg gtttttcttc tggaaggggt ttgtcttcca gagtggaaac
     4021 cacccactta tgaaaatctt ctgaggtcat tctgtcgcag tgccacttct ttgcatgccc
     4081 tagaatgtct ttgtctgagg tcaccacgcg aatgtcagcg gggcgggcgt tgtgtttgat
     4141 gaactgtttg atgatgtgat ctgcggaaag atcgtggctg taaatgactt tgatgccaga
     4201 aacgatttcg ctttctgtag catctccttt gttcttcttg ccatcaaaga ccaggtgaat
     4261 gtccagaggc ttcttccact tctttgcgaa gtcgtagacg atttccagta gtccgcgtct
     4321 ggcctctgcc agtctgccct gcgccatgtg ctcttcgagc actggaaatt tgtagatcac
     4381 gttgaatgca tctaccagga gcatcatggc tttagattca cttgacgtgc cgcaggcttt
     4441 gtttctggtt tcttatgatt ctctggctca aggcgtttca tatcattggc ttcactgcct
     4501 ggtttgcggg aatcttctat atctggcgtc tctttgtgta tcacgcagaa acgacttcag
     4561 aagatgttcg caaccagctt gcgatcatgg aatacaaact ctacaaatac atcatgcggc
     4621 cagccatggt cgtcactttg ttttttggtt ttgctttgct ctatttgcag tgggaagctt
     4681 tttttagatc cgcctggatc tggttgaagc tctgtcttgt cgccttagtg cttgcaaatc
     4741 atttcatggc agaggcgtat cgcaagaagc tggcccgcgg aatggcgtat aaatccagga
     4801 tctttcgcat tctcaatgaa atgcccactg tgcttttgat tggaattgtg ctgctggtgg
     4861 tgctcaagcc tttttaagtg gacaggaaag ctgtttttcg cgaaatcgac tgacgttgaa
     4921 attttccgaa ttacacatcc atcctgacat cattcgggcg tgcacagacg ccggttttga
     4981 aacacttact gaaattcagg aaaaatgcct gatccctgcc acgcagggcc gcgatatcgc
     5041 gggcatctca caaacgggaa caggcaaaac agtcgcattc cttcttcctc tgctggatcg
     5101 aattgtaaaa gacaatcttc ctggtccggc agctctcatc atcactccta ctcgcgaact
     5161 ctgtttgcaa attgcggaag aagctctgaa gcttacgaag cacggtccag tcagcgtcag
     5221 cgccgtttac gggggagaag gttatcgcaa gcaggaagaa gagctcgcga agaaccctca
     5281 gatcatcgta gcaacgcccg gacgtctcat cgactatatc aaacagggca aaatcgatct
     5341 caacaagttg cacttcctgg tgcttgatga agcagaccgc atgttcgaca tgggtttcat
     5401 tcgcgacatt ggctttatca tgaagaaggt tccgcgcgaa gcgcaaacca tgcttttctc
     5461 cgctactctg tcttactacg ttatgcgact tgcggcggac ttcatgaacg atcctgtcga
     5521 agttcgcata gaagcagaaa cagttgctgt ggacaaaatt gatcagcagc tttttcatct
     5581 tggcaaagac gagaagatgc cgtatctgat caaccaggta ctcgccgttc ctggaattcg
     5641 tgccatcgta tttacgaacc ttcgcgttta cgtttctacc attgtaaaca atctgcgtcg
     5701 ctatggagtg catgcaaccg gtctttcctc catgctcgat cagaaaaaga gagttcgtct
     5761 tctcaaagat ttcaaactgg gtaaatacag tgtgctcgtt gcaacagacg tggccagccg
     5821 tggtctagac gtagacgata tcactcacgt ttttaactat gatctccctc aagatgcgga
     5881 aagttacgtg catcgcattg gcagaacagc gcgcgctggg cgcagtggca taagcatcag
     5941 ctattgcagc gaaatggact acgaagcact tcccagagtg gaatcgtatc tggggaagaa
     6001 gatcccggtg aagactgtgg atgtggaact tttgaaattt cccgcaggcc agttcactcc
     6061 tttccaggaa gctcacggag aacgtgtgga aggagaagac agatcagatc gcgactcgaa
     6121 cagagacggg cgtggcggag gaagaaatcg cggaggccgc aagcgcaaag ggcaggatcg
     6181 tccgggcttt gctccggctc gcggtagaga tagaaatgat caagatcctt cgaaagaagg
     6241 tggagcgcgt gctggcgtaa attcggatcg caatcagaaa gatgatcgcg gaaggcgaga
     6301 cgatcgcgga cgcagagatt cgcgtgatag aagagatgat cgcggtaggc gcgacggcca
     6361 tcctggacag gatcgtggca gaaatgatcg acacggtcag ggaaatcgtg gtcgggatgg
     6421 cggtcgccct ggaaggggag acggtcgcca tggttcgcat cgtcatcgcg atcgcgatcg
     6481 caccagacgt gaaagtgttc ctcaaaagaa accaggaatc attgctcgca ttctttctat
     6541 tttcaagaag aagaaaccgg aagcttaggt tccagggttg aatcgggctg actcaggccg
     6601 ggccaagaga aaagacccgt tctttgagtg agatgacggc cgcgccggat ggctcttctg
     6661 gtctgtatgc gcaatcttca aaaaccttgc ctgcctgcgc aagtatctca ccttctctct
     6721 ctgtgttgag agtagatgca atttctcttg gagtctggta ttcgtggaaa tcaagcaggc
     6781 cttccgcctt gaggcgattg catgccagtt gaaataagag cattcccgct cttctgaaat
     6841 ttccagcagc cagaagctgc tcaatgactt tgaccagatc ctgatccgga agatgtgctt
     6901 ccggtaaatc cacaaccaca gatactttca tctcaggcgt ctcttccagg acttcttctt
     6961 ctgccggtct tctttcgagg atgcgtcgaa caatgagata gatcgcagcc cctgcaagaa
     7021 tggcggccac agcatacacg agctgtgtca aatctggcag cgcgaagccc gatctactcc
     7081 gttcttttgg cttttctaaa tatggtttga tatcgtccgg accgtaggcg cgataatctt
     7141 cttttggatt gtcgaaccag ggcatgtcct ttctccattc aggcggatct gcgtacagac
     7201 ccacagtgaa gaacaggaaa acgaatgaaa ggccagtctt caggaacgtt agacgacatt
     7261 gagacatgat caagcgctcc tcctttcgta tctggcttcc ggttcctgtt gggtttcctg
     7321 aatccctctt gcaagaagca attctatgtc ccatccttct cccgcgattc tagtatctaa
     7381 atatagaaga aagcgtacca ggcaaaagta ggcgatagaa ggaaagaaga ccagctggaa
     7441 taccactcca gtgggagcaa atcctggaga tggaatattc ttttccgccc aaccacccat
     7501 caactctcgc atagagctta agccgtagac tgtagagacc aggcctgcca gaaacatcag
     7561 gcaagaaaaa acaaaaaagc caacgattct gtctccctga ccgcgggcca atgccgccgc
     7621 acgcaatcca agtggagctc ccctcagtct ttccagtatg atcacttctc ccatgaagaa
     7681 atgagtgaac agtgcgcgca ccggaggaat gataaatgga aaaagcaacg tgtagtacag
     7741 agtattgaga atggccatgc gtcctatgta taccggggcc gtttgctttg tatctgccag
     7801 aacatctctg aatcttggtt taagctcaaa tacaatgcgt ccattcagtg cattgaaaat
     7861 catttgtaga acagatgatt caaacaaaag aagaagcacg atcattccat tggccagaac
     7921 gtcttctttt acgagttttg ttacaccgta gttgattgcg atgcaactga ttgtaaaagg
     7981 gaacaggatc cttccatatc tccagtggtt ggattttagc acgatcactg caagatcaaa
     8041 gatttcatcc agtcttcgcc tgcgcagtgg aatgcgaaga tcgagtagat tcacttgcct
     8101 cttcctcccg caaggaagta ggcgagcgtc agtagagtgg tcacgccgcc cagggcaagc
     8161 ttcacgttta ctcccggatt ccagggacca aggaacgcct cgatgaatgc ggcgaagaaa
     8221 gtaagcatgg ccgcgctata gagcgctggc atcgcagtca aagattcctc gcgaattgcc
     8281 agaagtcttg accttgcacc cggtgcaatg aggcaaaatc caattcgtac tcctgcagct
     8341 gcggacagac cgatcgcagt gagttcaaag ggagcgtgcg cggcagccca cgagactatg
     8401 tgatctctcg caggtgtgga catgagatag ccaatgatcg ttcctagaaa cactgcattg
     8461 taaagagtga agaacaagga gccaatccca acaagcgctc ccaggccaaa cgtgatcaaa
     8521 ttcaagccga tattgtttgc aatgtagaag ccgcttgccc ccacaagatc gcctaacttt
     8581 atttctccgc gttctccctc gtgcatgtca tggtattctt ccagaactgc ttctccaacc
     8641 acctgagctg caaaaggtct ggatacatac gccattccag cgcacagaaa aaaaggaaca
     8701 tagaaaacca ggaggcagat tcgcacatag acatctttgc ggatcgcagc cggaactgtt
     8761 tcaaaaatat atcttcttac atccgtgagc tttcttcgcg ggatagaata caggactgag
     8821 tgaccgcgac tcacgaggcc ttcgagtcgg gcctgttgca tgcgcgaaag cctgaatgcc
     8881 tgggccacgc tcaggtctgt actcactgcg cgatacaatc gtgcaaattt cagaatctcg
     8941 cgcacgggca ggcgaaactt tctttccaga tccggaagca tcttttccag atcatcccaa
     9001 tccttgctac gctgttctat gaaaatcgca ggcgtcatct tcgagtttta gtcttccgta
     9061 agttttctgt ggcaggcaag tagaagctgt tcggaagtgc attcctggtg tcctgcaagc
     9121 cattccagtc caggtctgac tctgtctgcg atctccattc ttcgagccgg gtgcattttc
     9181 tcgtacctgc ttgcaaagtc gtgtaacgcc tgcaccaggg tggcggaagg tagaattcta
     9241 aaagagattt ttctcaccag atcttccggc acgcgtactg tgattgcttt ttctttttca
     9301 gttggaattt cgcgaatcac aatggttccg gccgccagat cgcccagtct gcgaaatcta
     9361 ggagcactca taacgctgga aatgagtccg atcgtatagg tgggaagata taggagccat
     9421 accagaaatt tgaatgggaa catgtctgcg atgcgcaaca tgtttcgaat caagatctgc
     9481 actacatcca cagcagttcc atcaatggaa atcactcgaa ggccaagagc cattttgcct
     9541 ggagtccttc ctttgttcag ccattcgaat gcgaagaagt agaaccattg
//
LOCUS       DAORFF010000066         9558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3349_length_9558_cov_3.403452, whole genome shotgun sequence.
ACCESSION   DAORFF010000066 DAORFF010000000
VERSION     DAORFF010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9558
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3349_length_9558_cov_3.403452"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="PKK76_04285"
     CDS             <1..1328
                     /locus_tag="PKK76_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL67714.1"
                     /translation="RRVLESAMNQVSSGHYAEDYRIVRPDGQIRWIRDTGFTRKNRNG
                     EVIGFAGVARDITDQKLMAEQILHSQKMESIGTLASGVAHDFNNLLGIIMGQTAWLEE
                     NTQGETRRRIESIQQTAERGAALVRQILTFARKGETLRRSVDINALLLEFRPLISQLF
                     PKNIHTEFHLDQNLPPLSLDSTQLHQVVMNLCVNARAAMPEGGDLIVSTRLVDQSEVR
                     THFIHAEAKQYVEVSVKDTGSGMKEEIRSRIFEPFFTTRPGGEGTGLGLAVASGIVES
                     HSGFIRVESEPGKGSIFSVYLPVPLAPGRRENPNTAPEKATSTTANHTILVVEDEPHL
                     LEIMKSVLSSHGYEVITAENGNAGLQLFRNGQFHAVVSDLSLPLMDGYALAREMKRIA
                     PSIPIIIASGFFDPILKLELEGAGIKHFLQKPYRVTDLIETVRSAIEAS"
     gene            complement(1450..2070)
                     /locus_tag="PKK76_04290"
     CDS             complement(1450..2070)
                     /locus_tag="PKK76_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67715.1"
                     /translation="MARLFSLPFLLYAAFLAALCVDVVRLGDLRLAHGLLVASLSLLK
                     GRHQLVQTGDVIREWFDSLESITKAFGACYDSKPLESEGLCRIVQSSLEEVEKLRKIK
                     NQNIFCETPPSVAVLAEEKGLALCVRELLTNAFKYSPEGSNIHITSYRTGNSVSLVVL
                     NDVEFPRAETPVCRAKWKMLYSNPSFAFTIHMMNAFAPRSWAWALG"
     gene            complement(2280..3233)
                     /locus_tag="PKK76_04295"
     CDS             complement(2280..3233)
                     /locus_tag="PKK76_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNL67716.1"
                     /translation="MARSQPRIGLALSGGGFRAAAFHIGVIKTLYDLGLYTSIRFVSS
                     VSGGAIVGAAIGLNQDRTVLDLLEVYLMNHSPLGSSFLRGLFDFSHTRTDKLADSYDE
                     ALYHGARLSDLKGFKTVINSVSATTGHPFYFEGGNAKARLGEYETGFVSADSFSVARA
                     VAASGAYPVFLSPLELTSSEYSPARTFLVDGGVYDNLGIHSLLREEDLDLLIVSDAGA
                     PLRRNETPGDGVFAIESTMDIMMEQMRGMQIQRLTHLEKGKRAIWFSIDSKGEGDNAE
                     ICSRIPTGLSALPRNDLSILQRHAETLLRARLSAYAPEWIS"
     gene            complement(3288..4271)
                     /locus_tag="PKK76_04300"
     CDS             complement(3288..4271)
                     /locus_tag="PKK76_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNL67717.1"
                     /translation="MTHPNQALFSGEKPFPVIPSCEHFAGNEKMIGKALQLQQEKGPV
                     FDITQDCEDGAPQGQEVEHAKMIVRITNSSENKLGMAGVRIHDHSNPHWKQDVDILVP
                     GAGDKLAYITIPKPTAASQVEEMIDYIRNAASKAGLKREIPIHVLVETHGALRDADKI
                     AAMPWMQVLDFGLMDFISGHFGAITLSNMKSPGQFDHRLISRAKANVVAAALANGIIP
                     AHNVTLDLKNFEQTKSDATRARYDFGFMRMWSIYPTQIDAIVQAMQPDHSEVEKGCAV
                     LLAAQDASWGPIQHEGELHDRATYRGFWNIVQRARLSGQALPAEAIKRWFA"
     gene            4325..5149
                     /locus_tag="PKK76_04305"
     CDS             4325..5149
                     /locus_tag="PKK76_04305"
                     /inference="COORDINATES: protein motif:HMM:NF021998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase CarF family protein"
                     /protein_id="HNL67718.1"
                     /translation="MSIKKDSKVRATSHECIHMENSAVKPDIIMEEDAFAHSAGPVHV
                     FFEHLATYTYPIVFTSTLALSVLEFNNARNLYWLLLLMPLGWLAADWITGIIHWFFDT
                     YGSQTMPVLGRSFIKPFRDHHIDPKGITTHSLTVTIGNTCIAAVPITFWILYPLLFGD
                     PTDAYRMTAFFLSFTIGGTVLTNQFHKWSHLDTPPLWIRTLQKGKIILGIVHHDVHHT
                     KPFDTFYCITNGWMNPILDRINFWRGMEKLLGWFGVKPYPGIAEERAINAGVAASR"
     gene            complement(5092..5856)
                     /locus_tag="PKK76_04310"
     CDS             complement(5092..5856)
                     /locus_tag="PKK76_04310"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNL67719.1"
                     /translation="MSHDFANILFAGPCNRFCPFCIGKELPDRANEENLNLYPLRNQD
                     AFIREVQRLDIREIVMTGTTTDPQLYRHEERLLSEMRRQLPHARFSVHTNGVLALRKL
                     EAFNSYDRACISFPSFRSDTYEKMMGSRIVPDLRSILRESRIPVKVSCVINEHNVHEL
                     DDFILEAHSIGVRRLVLRKLFGETRTWDVLKDRIPESHYRDNPVYNVDGMEVTYWDFD
                     RTTSKSINLFPDGTIGPSYLLAATPAFMARSSAIPG"
     gene            complement(5853..7331)
                     /locus_tag="PKK76_04315"
     CDS             complement(5853..7331)
                     /locus_tag="PKK76_04315"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNL67720.1"
                     /translation="MIPWNKPAPADVNRQDPENVFAAGTRNHHISNTAYPIAHNVTWK
                     PYRVDRNANASLVESAFADAETISLYSHIPFCETRCYFCEYTVVGKKELDAASDYMKL
                     LSRELAMFRSLLGERSLSGFDIGGGTPSFVNAPLMAEHVDEVRKAFRTAPGFAISIET
                     TPRIASTEPEKMKAYADMGIERISMGIQVVQPDLLKHLGRSENGLDHHRKACDHIRGA
                     GFKKFNIDLMYGFAKQSDQSWEATLRHAIALEPDYITLYRMRYKLTRISAEAADVTLE
                     PVLRQAKLASEMLRSAGYIANPGKNTYSRIPKDTGTSAYLTRRVIEGSTYLGLGLGAQ
                     TFTDTTISYNDGAASKSIAPYAKSIAEGRLPIQDLYHLPLEHMMAKMIAVSFYFGEIR
                     ADAFEKKFKVSLEEAFPREIEFALKEGLMHWSISENGQEIPGLDRRALSLTPHGARNF
                     NGTIALFFAPSVQSYLIHRQSQADMHRHAASAQLVADKNDAA"
     gene            complement(7331..8950)
                     /locus_tag="PKK76_04320"
     CDS             complement(7331..8950)
                     /locus_tag="PKK76_04320"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNL67721.1"
                     /translation="MSGTIVILGGAMAGPTAAARAREINENARIILIEQGTRVSYAAC
                     GLSYYLSGEVQSLEDLNHERSDFFRQVYNIEVWTETEAIRLDSIKKILTVKQSKGEMD
                     LSYDSLIFAAGGSSIAPLEIQGVENQTVFRTLDDLVKIRSALDSGKKKFVILGGGPIG
                     LEAADGLIRAGAEVTIVEKGPQILGTFGDKISRVVRGILETSANVITSARDESFEIHE
                     GRITSVTVDGRRIEADYVISAMGIVPRTDILKEAGAGIHSDGTVVINESCATTLKDVY
                     ACGVCVSVPHSGSFSWTPQASVADKTAQVAGENAAGGDAKLASFVGSLLLRLPNISAG
                     RVGPSFRNLGLAAGNRLGRAFVHAYDVEPYMPHAKPLMLELFYEKESGKVLALEAAGS
                     GVERRLDAAAVAIAGSLTIQQLAKLDLAYSPALGTARDALNVAATVAVQAERGRTATV
                     EPADLQARTQDYCIVDTGKKARGSVHLHIPLEELRGKIEEIRSALLKSGARQIACLSE
                     TGRRGHLAVRILAAHEIPAVNILGGARLSEF"
     gene            complement(8987..9268)
                     /gene="hisE"
                     /locus_tag="PKK76_04325"
     CDS             complement(8987..9268)
                     /gene="hisE"
                     /locus_tag="PKK76_04325"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HNL67722.1"
                     /translation="MKFLEDLEKLLYQRKHELPDGSYTTTLFKGGEDRILKKVAEESG
                     EVIIAVKNSDAKEITHEAADLIFHLLVALVQKGVPLSAIEAELISRHKK"
     gene            complement(9265..>9558)
                     /locus_tag="PKK76_04330"
     CDS             complement(9265..>9558)
                     /locus_tag="PKK76_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNL67723.1"
                     /translation="EWAREAEDRGAGEILLTSMDRDGTKDGFDIELTAAVRSAVRIPV
                     IASGGAGNADHLVEAAKDAHADAVLAASIFHFRETTVDSVKKIMRERGVQVRV"
BASE COUNT         2342 a         2519 c         2308 g         2389 t
ORIGIN      
        1 gacgccgcgt gctcgagagt gcgatgaatc aagtctcatc cggtcactac gcagaggatt
       61 acagaatcgt aagaccggac ggacaaattc gctggatccg cgacactgga tttactcgca
      121 agaatcgcaa cggagaagtg attggtttcg caggcgtcgc tcgcgatatc acggaccaaa
      181 aactcatggc agaacagatt ttacattcgc agaagatgga aagcattggc acacttgcaa
      241 gcggagtagc tcacgacttc aacaatcttc tgggcatcat catgggtcag acagcctggc
      301 tggaagaaaa tacacaggga gaaaccagaa ggcgaattga gtccatccag caaactgcgg
      361 aaagaggcgc agcgctggtc agacaaatcc tcacctttgc acgcaaaggc gaaactctcc
      421 gccgatctgt ggacatcaat gctttgttgc tcgagtttcg gccgcttatc agtcagctct
      481 ttccaaaaaa cattcacacc gaattccatc tcgaccagaa cctgcctcct ctctcgctcg
      541 attcaacgca gcttcaccag gtagtcatga atctatgcgt caacgctcgc gctgcgatgc
      601 ccgagggcgg agatctcatt gtatccactc gccttgtgga ccagagcgag gtgcgcactc
      661 acttcattca tgccgaggct aagcaatatg tagaggtctc tgtcaaagac acggggtctg
      721 gaatgaaaga ggagattcgt tccaggatct ttgagccctt cttcaccact cgcccgggcg
      781 gagaagggac cggacttgga ctggcagtag cttccggcat cgttgaatct cactccggct
      841 tcatacgcgt tgagagcgaa cctggaaaag ggagcatctt ctctgtgtac ctgcctgtac
      901 ctcttgcacc tggaagacgg gagaatccaa acactgcgcc cgaaaaggcc acttcaacaa
      961 ccgcgaatca tactatcctc gttgtggaag acgagcctca cctgcttgag atcatgaaga
     1021 gtgtgctttc atcgcatgga tacgaagtga tcacggctga aaatggaaat gcggggcttc
     1081 aattgtttcg gaatggacaa tttcacgcgg tcgtatctga tctcagtttg cctttgatgg
     1141 acggatacgc tctggctcga gaaatgaaac gaatcgcacc gtctattccc atcatcattg
     1201 caagcggatt ctttgatcca atcctgaaac tggaactgga gggagcggga atcaagcact
     1261 tcttacagaa accttatcgc gtaactgacc tcattgaaac agtacgatcc gcgatagaag
     1321 cttcttgaga agttctcaga atcaattccg ctgccactct ccgtcttgga accagaccgg
     1381 acgcatgatc agtgatctca tagacgtaga cgctgcctcc gaattgattg acggcgtttt
     1441 gcacaacagt caacccaatg cccatgccca gctcctcgga gcgaaagcgt tcatcatatg
     1501 tattgtgaat gcgaaagaag ggttcgaata cagcattttc catttcgcgc ggcacaccgg
     1561 tgtttctgcc cgtggaaatt caacatcgtt tagaaccact agcgaaactg aatttcctgt
     1621 tctgtaggaa gtgatgtgga tgttgctccc ttcaggcgaa tacttaaaag cgttggtcaa
     1681 aagctcgcga acgcacagag caagtccttt ctcttccgca agtacggcca cggagggtgg
     1741 tgtttcgcaa aatatattct gattcttgat ctttctcagt ttttccactt cctccaaaga
     1801 gctttggaca atgcggcaaa gtccttcgct ttccagtggt tttgaatcgt agcaagctcc
     1861 aaaggctttc gtgatgcttt cgagagaatc gaaccactcg cgaatcacat ctcccgtttg
     1921 aaccagttga tgtcttcctt tgagaagtga cagggaagcc acgagtagac cgtgagccag
     1981 acgcaggtct cccagacgaa ctacatccac gcacagcgcg gcgagaaacg cggcgtacaa
     2041 caagaatggc aaactgaaaa gccgggccat ccccggaaga ttatcacatt tgttttccgg
     2101 gtcaaggggt agacgtacca gtgtgaccag gtatttttac ggacaaatcc tcatagagca
     2161 gacaatctgg cttcatgcaa acgtactcca tggacttgaa gttcttcctc gcagggttga
     2221 tcctttcggc cgcactcatt gcaatccacg tagccgttca gtgatcagac tctggccact
     2281 caggaaatcc attcgggagc gtaggcggat agccgggccc gaagcagagt ttctgcatgt
     2341 ctttgcaaaa tagaaagatc attgcgaggg agagcagaaa ggccggttgg aattcgagaa
     2401 cagatctccg cgttgtcgcc ttctcctttg ctatcgatag aaaaccagat cgcacgtttt
     2461 cccttttcca gatgagtcaa tctctggatc tgcatgccgc gcatctgttc catcatgatg
     2521 tccatcgtgc tttcaatggc aaacacacca tcccccggtg tttcatttct tcgaagagga
     2581 gcgcccgcat cgctcacaat caatagatct agatcttcct cgcgaagaag agaatgaatt
     2641 cccaggttgt catacactcc accatcgacg aggaaagtgc gcgcaggcga atattcgctt
     2701 gaggtaagct ccagaggaga cagaaataca ggatacgctc cgcttgcagc cactgcacgt
     2761 gccacagaaa acgaatcagc gcttacaaaa ccagtttcgt attcacccag ccgcgccttt
     2821 gcatttccac cttcaaagta gaaaggatga cccgtcgttg cactcacaga attgatgact
     2881 gttttgaatc cttttagatc cgaaagtcta gcgccgtgat agagtgcctc atcgtatgaa
     2941 tcagccagtt tgtctgtacg ggtatgcgaa aaatcaaaga ggccacggag aaaggaactt
     3001 cccagtggcg aatgattcat gagatagact tctagaagat caaggaccgt gcgatcttga
     3061 ttcagtccaa tcgccgcgcc tacaatcgca cctccgctta cgcttgaaac aaatcgaata
     3121 gaagtataga gaccaagatc gtataacgtt ttgattacgc cgatatgaaa agcagcagcc
     3181 ctgaatccac cgccagaaag tgccaggcct attcgaggtt gggagcgggc catctaaatt
     3241 gagtgaggaa tatcggagcg gcagtccgcc gctccgtgaa acagtggtta cgcgaaccag
     3301 cgcttgattg cctctgccgg cagagcctga ccggaaagac gcgctctttg gacaatattc
     3361 cagaatccgc ggtaggtagc gcggtcatgg agttcgcctt catgctggat tggcccccag
     3421 cttgcgtctt gagccgcgag aagcactgcg catccctttt ccacttctga atgatccggc
     3481 tgcattgcct gaacaatcgc gtcaatttga gtagggtaaa tggaccacat gcgcataaaa
     3541 ccaaagtcat agcgcgcgcg agttgcatct gatttcgttt gctcaaagtt cttgagatcc
     3601 agagtaacgt tgtgtgccgg aatgattcca tttgcaagag ctgccgcaac tacgtttgct
     3661 ttggcgcgcg aaatcaatct gtgatcaaac tgaccggggc ttttcatgtt ggaaagagtg
     3721 atggctccaa aatggcctga gatgaaatcc atcaaaccaa agtcgagaac ctgcatccat
     3781 ggcatggctg caatcttgtc tgcatcccga agtgcaccat gagtttccac aagtacgtga
     3841 attggaatct cgcgcttcag acccgctttg gaggcggcgt tgcgaatgta gtcgatcatc
     3901 tcttccacct gggaagcagc tgttggcttt ggaattgtga tgtaagcgag tttgtctcct
     3961 gcacctggta ccagaatatc tacatcctgt ttccagtgtg gattggagtg atcatggatg
     4021 cgcacacctg ccatgccgag tttgttttca gaagagttgg tgatgcgtac gatcattttc
     4081 gcatgttcca cttcctggcc ttgaggagcg ccgtcttcac agtcctgagt aatatcgaat
     4141 actggtcctt tctcctgctg cagctggaga gccttgccga tcatcttttc gtttcctgcg
     4201 aaatgttcgc aacttgggat cactggaaag ggcttttcgc ccgaaaacaa tgcctgattg
     4261 gggtgagtca tttttgaagc ctcgatgttt gttcttcctt cctgattttg gaaaggaggg
     4321 ccctgtgtcc ataaaaaagg attccaaagt ccgagccact tcccatgagt gcattcatat
     4381 ggagaattcg gctgtcaaac ccgacataat catggaagag gatgcatttg cgcattctgc
     4441 ggggccagtg catgtttttt tcgagcatct ggctacctac acgtatccca ttgtgtttac
     4501 gagcactctt gctctttcgg tgcttgagtt taacaacgcg cgaaatctct actggctact
     4561 cttgctaatg cctctgggat ggctggcggc agactggatc acaggaatca tccactggtt
     4621 ctttgataca tacggatctc agactatgcc agtgcttgga agatcgttca tcaaaccatt
     4681 tcgcgatcat cacatagatc caaaggggat taccactcac agccttacag tgactattgg
     4741 caatacatgc attgctgccg ttcccatcac attctggatt ctttatccgt tgttattcgg
     4801 tgatccaaca gatgcgtacc gcatgactgc tttctttttg agtttcacca ttggcgggac
     4861 agtgctcacc aatcagtttc acaaatggtc gcacctggat actccgcctc tttggattcg
     4921 cacacttcag aaagggaaga ttatactcgg cattgtccat catgacgttc accacacaaa
     4981 gccgtttgat actttctatt gcatcaccaa cggatggatg aatccgattc tggatcgaat
     5041 caatttctgg cgcggtatgg agaaacttct cggatggttt ggcgtcaaac cctatcccgg
     5101 aatcgcagaa gaacgagcca taaatgcagg cgttgccgca agcagataac tcggtccaat
     5161 ggttccatcc gggaacagat tgatgctttt gcttgtggtg cgatcaaagt cccagtacgt
     5221 tacttccatt ccgtccacgt tgtaaaccgg attgtccctg taatgggatt ctggaatgcg
     5281 atcctttagc acatcccagg ttctggtttc cccaaaaagc tttcgcagaa ccagacgacg
     5341 aactcctatg ctgtgagctt ccagaatgaa atcatccaat tcgtgcacgt tatgctcgtt
     5401 gatgacgcaa ctgaccttca ccgggatccg tgattccctg agaatggagc gaagatccgg
     5461 gacaatcctt gaacccatca ttttctcata tgtatcggaa cgaaagcttg ggaaagaaat
     5521 gcaggctctg tcataggaat tgaacgcttc gagtttacga agagccagca ctccgtttgt
     5581 gtgaacagag aaacgagcgt gaggcaattg ccttcgcatc tcagacaaca gcctttcttc
     5641 atgacgatag agctgcggat ctgtagtggt cccagtcatc acgatttctc taatgtccag
     5701 tctctgtact tcgcgaatga atgcgtcctg gtttcgaagt ggatacagat tcagattctc
     5761 ttcattggcc cgatccggaa gctccttacc aatgcagaaa ggacagaaac gattgcatgg
     5821 cccggcaaaa agtatgtttg cgaaatcgtg gctcatgctg cgtcgttttt atccgccacc
     5881 agttgcgcag atgccgcatg acggtgcatg tctgcctgag actgacgatg gatcagataa
     5941 gactgaacgg atggcgcaaa gaaaagagca atggttccgt tgaaatttcg ggcaccgtga
     6001 ggtgtaagag agagcgcgcg acgatccaat ccagggattt cctgcccgtt ttcggaaatg
     6061 ctccagtgca tgagaccttc tttcaaagca aattcgatct cacgcggaaa tgcttcctca
     6121 agagaaacct tgaacttttt ctcaaaagcg tccgcgcgaa tctcaccaaa atagaaactc
     6181 acagcaatca ttttggccat catatgctcc agaggcagat gatacagatc ctgaatcggc
     6241 aaacggcctt ccgcgatgct ctttgcgtaa ggagcaatgg atttggaagc ggctccgtca
     6301 ttgtaggaaa tggtggtatc tgtgaatgtc tgtgcgccaa gccccaggcc aaggtaggta
     6361 gatccttcta tcacgcgacg cgtcaaatac gcgctggtcc ccgtgtcttt tggaattcta
     6421 gaatacgtgt tcttgcccgg attggcaatg taacccgcag acctgagcat ttcactggcg
     6481 agttttgcct gacgcagcac aggctcaaga gtgacatccg ctgcttctgc ggaaatgcga
     6541 gtgagcttgt atcgcatgcg atagagagta atgtaatccg gctcgagtgc aatcgcatga
     6601 cgaagagtcg cttcccagga ctggtcactt tgctttgcga atccgtacat cagatcaatg
     6661 ttgaatttct tgaagcctgc ccctcgaatg tggtcacacg cttttctatg gtggtccagg
     6721 ccattttcag atcttcccag atgcttcagt aaatccggct gcaccacctg gatccccatg
     6781 ctaatgcgct caattcccat gtccgcgtat gctttcatct tctctggttc tgtggacgca
     6841 atcctgggag tggtttcaat actgatggca aaaccaggag cagtgcgaaa agctttccgg
     6901 acctcatcca catgctctgc catgagtggc gcatttacaa aagaaggagt tccgccgcct
     6961 atatcaaagc cagatagact ccgctctccg agtagactgc gaaacatggc aagttcccgc
     7021 gaaagaagtt tcatatagtc agaggcggca tcgagctcct tttttccgac cacggtatac
     7081 tcgcaaaaat aacacctggt ctcgcaaaat ggaatgtggg aatagagcga gatcgtctcc
     7141 gcgtccgcaa acgctgattc taccaggctg gcgttagcat ttctgtccac tcgatatggc
     7201 ttccaagtaa cgttatgcgc tattggatag gctgtattgg atatgtgatg atttctggtg
     7261 cctgcggcaa aaacattttc cggatcttgc cggtttacat ccgctggcgc gggcttattc
     7321 cagggaatca tcagaattca gaaagcctcg ctccgcccag aatattgacg gccgggattt
     7381 cgtgtgctgc aagaatcctc acagccagat gacctcgcct tcctgtttcg ctcagacatg
     7441 cgatctgtct tgctcccgat ttaagaagtg cggacctgat ttcttcgatt ttgccgcgca
     7501 gctcttctaa agggatgtgc agatgaacac tcccgcgcgc tttttttcca gtgtccacga
     7561 tgcagtagtc ctgggtgcgg gcctggagat ctgccggttc cacagtggcc gtgcgccctc
     7621 gttccgcctg aactgccacg gtggccgcta cattgagagc gtctctggct gttcccagcg
     7681 ccggactgta tgccagatca agtttcgcaa gctgctggat ggtcagagaa cctgcaatcg
     7741 ccactgcggc cgcgtccaga cgtctctcta cgcctgatcc agctgcctcg agtgcgagga
     7801 ccttgcccga ttccttttca taaaacagct cgagcatcaa aggctttgca tgaggcatgt
     7861 atggctccac atcataggca tgaacaaacg cgcgacccag cctgttgcca gcagccaacc
     7921 ccagatttct aaaagacgga ccaactcttc ccgcggagat gttgggaagg cgaagcaaga
     7981 gagacccaac aaacgatgcg agctttgcat ccccaccggc cgcgttttct cccgccacct
     8041 gcgcggtctt atccgccacg gaagcctgag gagtccagga aaagcttccg gaatgcggaa
     8101 cactcacgca cactccgcag gcatacacgt cctttagagt tgtggcgcat gattcattga
     8161 tcaccactgt gccatccgaa tgaatcccgg cgcccgcctc tttcaagata tctgtgcggg
     8221 gaacgatgcc catggcggaa atcacatagt ctgcttcaat tctgcggccg tccacagtta
     8281 cagacgttat gcggccttca tggatctcaa acgattcatc tctcgcagat gtaatgacat
     8341 tcgcggaggt ttctagaatt ccacgcacga ctctagaaat cttatctccg aacgttccca
     8401 gaatctgtgg acctttttct acaatagtaa cttctgcgcc ggcgcggatg agcccgtctg
     8461 cggcctcaag gccaattggc cctcctccaa gaattacaaa tttctttttt cccgaatcga
     8521 gagcggagcg aatcttcacc agatcatcga gcgtgcgaaa tacagtctgg ttctccactc
     8581 cctggatttc cagaggtgca atgctcgatc cacccgcggc aaagatcagt gaatcatagg
     8641 aaagatccat ttcccccttt gattgtttga ctgtgaggat tttcttaatg ctgtcgagac
     8701 gaatggcttc tgtctccgtc cacacctcaa tgttgtatac ctgtctgaaa aaatcagaac
     8761 gctcgtgatt caaatcctca agagattgaa cctctcccga aaggtaataa gaaagaccgc
     8821 aggccgcata gctgacgcgt gttccctgct caatcaagat gatccgcgca ttttcattga
     8881 tttcgcgtgc acgggcagcc gcagttggac ctgccatggc accacccaga atgacaatcg
     8941 taccggacat agactccccc gaactataaa ctttcttaaa gattgactat ttcttgtgac
     9001 gcgagatgag ctcagcctcg atcgcgctca gcggaactcc cttttgcacg agagcaacga
     9061 gcaaatgaaa gatcaagtcc gccgcttcat gagtgatttc cttcgcatcg ctattcttta
     9121 ctgcgatgat cacttccccg gactcttctg ccactttctt caggatgcga tcttccccgc
     9181 ccttgaagag agtcgtagtg taggaaccgt ccggcaattc gtgcttacgc tggtagagta
     9241 atttctcaag atcttccagg aatttcatac tctgacctgc actccacgtt ctctcatgat
     9301 cttcttcacg ctgtccactg ttgtttctct aaagtgaaaa atggaggcag ccagaactgc
     9361 gtctgcatgt gcgtcttttg ctgcttccac aagatgatct gcgttcccgg cgccgccgga
     9421 tgcgatcact ggaattctaa ctgcactacg cacagccgca gtcagctcaa tgtcaaagcc
     9481 gtcttttgtg ccatcacgat ccatgctggt gagcaaaatc tcaccggctc cgcgatcctc
     9541 tgcctctctg gcccattc
//
LOCUS       DAORFF010000067         9524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3366_length_9524_cov_4.168444, whole genome shotgun sequence.
ACCESSION   DAORFF010000067 DAORFF010000000
VERSION     DAORFF010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3366_length_9524_cov_4.168444"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PKK76_04335"
     CDS             <1..307
                     /locus_tag="PKK76_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNL67724.1"
                     /translation="VMFEFARMIASTTLQFRERLRALTLLNVQQRLESFLSELGARKQ
                     AVALPLPKNQIASLIGSTPESVSRAFRILVDEGKLKVDGERYHLEGSGGSKADAASV"
     gene            complement(326..1441)
                     /locus_tag="PKK76_04340"
     CDS             complement(326..1441)
                     /locus_tag="PKK76_04340"
                     /inference="COORDINATES: protein motif:HMM:NF019404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HNL67725.1"
                     /translation="MAIEETRVVGPWEQMKNSFGGMLIGLLLLPGAFFIVYISTCREQ
                     ASVEMKGALPASEAAKAAADNKPVYATGKLDSSPVGDPQFVKPGKYLKLSRSAQIMAW
                     VHKETTKTEGSGTTKKETKTYDCKLEWTSSPDLNVGSKKGCEGKTNFSATYSSADFAA
                     DSVTINSSGTTYSLNPSAIDPVKLPGAKVQKSDLTRDMTEYSGAFYPEAACATSSPKA
                     GCEKITFSGTSYEAGKDYTAVGKLVGNQFEEYSTGKSGILAGHWLKLGPGSFKETMSA
                     VSSADSGWTIGLYLGSVFCFWLGLVLLTGPILQLIEFIPIIGGFGEGLIKVIFGVFSF
                     VIMGLAFLIIKYFLFILLGLALIAGFLIYKKKSAPAA"
     gene            complement(1549..4002)
                     /gene="lon"
                     /locus_tag="PKK76_04345"
     CDS             complement(1549..4002)
                     /gene="lon"
                     /locus_tag="PKK76_04345"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNL67726.1"
                     /translation="MSSSIRYTDQNITEIDKSRIPLELPVIPLRNAVVLPGVLLPIFV
                     GRNESIELIEKNGKPGSLVALFTQISAQEENVDEKNLYRTGSLAEIHRVIPMGEGGYQ
                     VFLQGVQKIELIEVVKTSPYLVGRVMPLGELNDSTDEQFLEFQAHVVRYVEAHPGIPD
                     EVTNFVRRLKNPSALANQVVFFSQKEIHEKIDFMKISSIAEKIRKVQEDLVEETNRMQ
                     MEREVREKVEQDTTKMQRDFYLRKQMETIRKELGEDDEADTDDIEKQLKEKWLPDDVR
                     KAVDKELKRFRRAQEGPQGGGFEVNQIRNWLELILELPFENPEPREILLSRAEEILNE
                     DHEGLEEVKKRILEYLAVEKQTGGSRAPILCLVGPPGVGKTSLARSVARALGRPLVRS
                     ALGGVRDEAEIRGHRRTYVGAMPGKILTAMKKAERRDAVFLLDEIDKTGTSYHGDPSA
                     ALLEVLDPEQNHSFEDHYLGLPYDLSRVLFVATANNADSIPLPLLDRMEIVNLSGYTL
                     AEKRQIARKHLVPTILNDLKLDADQISFTDGAIEKVITAHTREAGVRSLKRKLEGIAR
                     KSIRKILEAREKNQSTGNEEKLTIREEDVKEMLGRDRFHRDMKEHLGKPGLAVGLAWT
                     PVGGDILYIEAASYPGKGDFKLSGQLGDVMKESAHAAFSFLKSRAQEIGISQEAFATR
                     DFHIHIPSGAIPKDGPSAGVTLLSALTSLLKGVPIAENIAMTGEISLRGRILPVGGIK
                     EKVLAARAAGIETVLLPEQNRPEFEEIPAHVTEGLRVEYYSEMMELLKRTVPRAFESN
                     TGSAGQTGYSMQASRRDEW"
     gene            4206..5300
                     /locus_tag="PKK76_04350"
     CDS             4206..5300
                     /locus_tag="PKK76_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNL67727.1"
                     /translation="MKYFFALAALAVLHPAFAAPLDAPPGVSSEDFQRAMSQSRNWQI
                     VNHAIYEAVRPSTVQIIAAGGTEVAANADPRMKDPFFRKFFGPPPSQEAPPSIGSGFI
                     LDDSGLVVTNLHVVRNAKNVQVILADGTKVPGVIRGSDEWTDIAVIQMKNAKNIRPAR
                     LGDSDKVYVGDYVVALGNPFGLQGTFSTGVVSAVARSVPGDPGMKFLQTDAAINPGNS
                     GGPLINLDGEVIGINRMIVSPAGGSVGIGFAIPINEVKKVIADIKANGTVVRPALGVQ
                     IENLSPEGKKTLGVETGVVILGVVPGSGADKAGLEQKDVITKVDGTIYDDTEPLRSYI
                     LTKRVGDKVTLEIVRNKVVQQLQIVLGKRQ"
     gene            5474..7045
                     /locus_tag="PKK76_04355"
     CDS             5474..7045
                     /locus_tag="PKK76_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNL67728.1"
                     /translation="MANAADLKARTEDIRTLASSIQEGGGARAKKLHESRGKLFVRDR
                     IQLLLDPGSPFLETGLFTAHQAYDFDVPSGGIVTGIGRIENTLCMIVANDATVKGGTY
                     LPLTVKKHLRAQEIALENRLPCIYLVDSGGAFLPMQDEVFPDKEHFGRIFYNQAVMSA
                     QGIPQIAVVMGSCTAGGAYIPAMSEETIIVRGNGTIFLGGPPLVKAATGEDVTAEDLG
                     GAHVHTHKSGVADHFAQNDEHALEITRRIVKHLNYRPAASAFAARFAARSPIYAPEEI
                     YGIIQKDLRKPYDIREIMARIVDGSEFHEFKENYATTIVTAFAHVFGFPVGIVANNGV
                     LFSESALKAAHFIELCSHRKIPLLFLQNITGFMVGRQYENAGIARDGAKMVTAVSNAN
                     VPKITVVVGGSYGAGNYGMCGRAYGPRFLWMWPNARISVMGAEQAANVLLTVKMEQIA
                     RERGQGLSQEEQIEFKKPILDEYGHKSHALYSSARLWDDGIIDPARTREVVGLSLAAC
                     TGSPVGEAPSSLFRM"
     gene            7121..9139
                     /locus_tag="PKK76_04360"
     CDS             7121..9139
                     /locus_tag="PKK76_04360"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HNL67729.1"
                     /translation="MKYPHLLSPLDLGFTTLRNRVLMGSMHTGLEDMPGGYERMAAFY
                     AERAEGGVGLIVTGGIAPNEAGKVNRTGSVLVNEEEANLHIPVTRAVHEHGGKIAMQI
                     LHTGRYGYHDKAVGASELRAPINVVKPHKLSHQEILSTIDDFARCTELARHAGYDGVE
                     IMGSEGYLINQFTAKRTNNRDDDWGGSLENRMRFPLEIIRAVRKRVGNDFIIVYRLSM
                     LELVEEGGNLDDVIRLAKEVEAAGVNILNTGIGWHEARVPTIAMMVPRAAFTWVTKNV
                     RQHVKIPLCTSNRINTPEIAEAVLANGDADMVSMARPLLADSHFVNKAAADKADEINT
                     CIACNQACLDHIFVGTTASCLVNPRACNETELNYIPATKKKRIAVIGAGPGGMSAAAV
                     AAERGHNVTLYDAAGDIGGQIHIARRIPGKEEFNETLRYFRTILKKFGVTQKLSTRVS
                     AADLIAEKYDEVILATGINPRKLTIPGSDRPNVLSYVDVVLHGKAVGKRVAVIGGGGI
                     GFDVTIYLTESESSADRKTAFLVEWGIDPSIKVSGGLLPEQKHPAAPRQVTMFKRSKG
                     KFGGNLGKTTGWIHRQTVEDRHVEQISGVTYEKIDDQGLHYSLKGQNHVLPVDTVVVC
                     AGQEPNQELLGPLKSAGVSVHVIGGALEAAELDAKRAIDQGARLAASL"
     gene            9228..>9524
                     /locus_tag="PKK76_04365"
     CDS             9228..>9524
                     /locus_tag="PKK76_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNL67730.1"
                     /translation="MNGGELIAQTLQLHGVRAVFTLIGGHISPILVSCKKAGVRVVDV
                     RHEVNTVFAADASARLTGIPGVAVVTAGPGVTNTLTALKNAQMAQSPLVLIGGAT"
BASE COUNT         2219 a         2363 c         2507 g         2435 t
ORIGIN      
        1 ggtgatgttt gaatttgcac ggatgattgc gagcaccact cttcagttcc gcgaaagact
       61 tcgcgctctg actttgctca acgtccagca gcgtcttgaa agttttctgt cagagcttgg
      121 tgcaagaaag caggctgtgg ctctgccttt gcccaagaat caaattgcgt ctctcattgg
      181 ttcgactccg gaatcggtga gtcgcgcatt tcggatactt gtggatgaag gaaaattgaa
      241 agtagatggt gagagatatc accttgaagg ttccggcgga agtaaagcag acgccgcttc
      301 tgtataaagc ggcgtctagc atctgttagg cggccggtgc gcttttcttc ttgtaaatca
      361 agaatcctgc aatcagagca agtccaagga ggataaacaa gaagtactta atgatcagga
      421 acgcaagtcc catgatcaca aaagagaaca ctccaaagat caccttgatg agaccttctc
      481 caaatccacc aatgatcggg atgaattcaa tcagctgaag gatgggtccc gtaagtagaa
      541 ccagtccaag ccagaaacag aagacggagc ccagatacag tccaatggtc cagcctgaat
      601 cagcactgga gaccgcagac atggtttctt tgaaagatcc cgggccaagt ttcaaccagt
      661 ggccagcgag gattccgctt tttccggtgg aatactcttc gaattgattt ccgaccagtt
      721 tacccactgc agtgtaatct tttcctgctt cataggaagt accggagaag gtgatctttt
      781 cgcatccagc tttaggactg gatgtggcac aggccgcttc agggtagaag gcgccgctgt
      841 attcagtcat gtcgcgcgtt agatctgatt tctgtacttt tgcgccaggc agtttgactg
      901 gatcaatggc agatggattg agggagtagg ttgtgccgga tgaattgatg gtcactgagt
      961 ctgctgcgaa atcggcgcta gaataagttg cggagaaatt tgttttccct tcgcatcctt
     1021 tcttgctgcc aacattcaaa tcaggagagc tggtccattc cagtttgcag tcgtacgtct
     1081 tggtctcttt cttggtagtg ccgctgcctt ctgttttcgt ggtttctttg tgaacccacg
     1141 ccatgatctg cgcacttctg gagagtttga gatacttgcc aggttttaca aactgcggat
     1201 cacctactgg tgatgaatcg agtttgcctg tcgcatagac tggcttgttg tctgccgcgg
     1261 ctttcgctgc ttctgaagca ggaagtgccc ctttcatttc cacagaggcc tgttccctgc
     1321 acgtggatat gtaaacgata aagaatgcgc ccggtaacag aagcaggcca atcagcatgc
     1381 ccccgaatga attcttcatt tgctcccagg gcccgaccac ccttgtttct tctattgcca
     1441 tgattgcctc cgcacgaatg tctgcggaaa atgcgcgaca tggagcagag attcaactaa
     1501 aatttgtttt ttgagaatgt gcgttgcacc aggcttcgca cactttcttt accattcgtc
     1561 tcgtcttgag gcctgcatgg agtaaccggt ctggcccgct gagcccgtgt ttgactcgaa
     1621 ggcgcgagga actgttcgct tcaacagttc catcatctca gaatagtact ccactcgcag
     1681 accttctgtc acgtgcgctg gaatctcttc aaattcaggg cgattctgtt ctggcagcag
     1741 aacggtttct attcctgccg cacgcgctgc gaggactttc tctttgatcc cacccaccgg
     1801 aagaattcgc ccgcgaaggg agatctcgcc tgtcatcgca atgttctcag caatcggcac
     1861 tcctttgagt agactggtca atgcgctgag aagtgtgacg cccgccgaag gtccgtcttt
     1921 tggaatcgct ccactcggga tgtgaatatg gaagtctctg gttgcgaaag cttcttgcga
     1981 gatcccaatc tcctgcgcgc ggctcttgag aaaagagaat gcggcgtgcg cagattcttt
     2041 catcacatcg cccagctgtc cggagagttt gaagtctcct tttccaggat acgaagccgc
     2101 ttctatatag agaatgtctc cgcccacagg ggtccatgca aggccgaccg caagtcctgg
     2161 cttcccgaga tgttctttca tgtcgcggtg gaagcgatcc cggcccagca tttctttgac
     2221 gtcctcttcg cgaatggtca gcttctcttc gttgccggta gactgattct tttcgcgcgc
     2281 ttctagaatc ttgcgaatcg atttgcgtgc gatcccttcg agttttcgct tgagggaacg
     2341 gactcctgct tcacgagtgt gcgccgtgat tactttttca atggctccgt ccgtgaacga
     2401 tatttgatct gcgtcgagtt tcagatcatt gagtatggtt gggacaagat gctttcttgc
     2461 aatctgacgc ttctctgcta gagtgtagcc ggacagattc acgatttcca ttctatcgag
     2521 caatggaagc ggaatgctgt ccgcgttgtt cgcggttgct acaaacagaa cacggctcaa
     2581 atcgtacgga aggcccaggt aatgatcttc aaagctgtga ttttgctctg gatccagtac
     2641 ttcgagaagt gccgcagatg gatctccgtg ataggatgtt cctgttttgt cgatttcatc
     2701 aagtaagaac acagcatctc tgcgctctgc tttcttcatc gcagtcagga tctttccagg
     2761 catcgcacct acgtacgttc ttctgtgacc ccggatttcc gcttcatcgc gcacgccgcc
     2821 cagagcggag cggaccagcg gtctaccgag agcacgtgcc actgatcgtg ccagagaagt
     2881 ttttcctacc cctggaggac ccacgaggca aagaatcggg gcgcgcgagc cacctgtttg
     2941 tttctctacc gcgagatatt caagaatccg ctttttcact tcttcaaggc cttcgtggtc
     3001 ttcgtttaga atctcttctg cgcggctgag aagaatttca cgtggttctg gattctcaaa
     3061 aggcaattcc agaattagtt ccagccagtt gcggatctgg ttgacttcga atcctccacc
     3121 ctgcgggcct tcttgcgcgc ggcggaatcg tttcaattct ttatcaaccg ctttgcgcac
     3181 atcgtctggc agccacttct ctttcagttg tttttcaata tcatctgtat ccgcttcatc
     3241 atcttcgccc agttccttgc ggatggtttc catctgtttg cgcagataga aatcgcgttg
     3301 catcttggtt gtgtcctgtt ctactttctc gcggacttcg cgttccattt gcatgcgatt
     3361 ggtttcttcc acaagatctt cctgaacctt gcgaatcttc tccgcgatgg aactgatctt
     3421 cataaaatcg atcttctcat ggatttcttt ctgagagaag aagactacct ggttggccag
     3481 tgcgctgggg tttttcagtc tgcgaacaaa attcgtaact tcgtctggga ttccgggatg
     3541 cgcttccacg tatcgaacca cgtgcgcctg gaattccaga aattgttcgt ccgtagaatc
     3601 gttgagttct ccgagtggca tgactcgtcc cacgaggtaa ggtgaagtct tcacaacttc
     3661 gatcagttca atcttttgga cgccttgcag aaagacctgg tatccgcctt ctcccatggg
     3721 gatcactctg tggatttcag cgagagaacc cgtgcgatac agattctttt cgtctacgtt
     3781 ctcttcctgc gccgaaattt gagtgaacag cgcaacgaga gatcctggct ttccattttt
     3841 ttctatcagt tcgatggact cgtttctgcc aacgaaaatg ggcaatagaa cccccggaag
     3901 gacgactgcg ttgcgcagtg ggatcacggg aagctcaagg gggatgcggc ttttgtcgat
     3961 ctcggtaatg ttctggtctg tgtagcgaat gctggaactc atggtaccct gcctttgcat
     4021 gacccgtgcc agcatcaaga ccggccaggg cttgtttctg tgccgtatgt gaaggaggcg
     4081 gggacatttt gtcatcccgc ttgccatttc catccggcgc agattatatt cacaaggaag
     4141 acgaggtttc tatggcgaac gagcgatttt tttcccaatc cgtgaggcat ttgcgtctat
     4201 atggcatgaa gtattttttc gctcttgcgg ctcttgcagt tttgcatccg gctttcgcgg
     4261 ccccgctcga tgctccaccc ggtgtatcca gcgaagactt tcagcgtgcg atgagtcaat
     4321 ctcgcaactg gcagatcgta aatcacgcga tctacgaagc agttcgtcct tccacagtgc
     4381 aaatcatcgc ggccggcgga acagaagtcg cggcgaacgc agatcctcgc atgaaggatc
     4441 cctttttcag aaaattcttt gggccacctc caagtcagga agctccgcct tctattggtt
     4501 ctggtttcat tctagacgat tcaggacttg ttgtaaccaa cctgcacgtt gtgcgaaatg
     4561 ctaagaacgt tcaggtgatt ctcgcggatg gtacgaaggt tcctggagtg attcgtggtt
     4621 cagacgagtg gacggacatc gccgtgattc agatgaagaa tgcgaagaac attcgtcctg
     4681 cgagactcgg agactcggac aaggtttatg tgggtgatta cgtagtcgct cttggaaatc
     4741 catttggact ccagggaact ttttcaacag gcgttgtatc tgcggtggcg cgttcggttc
     4801 caggagatcc tggaatgaaa tttcttcaaa cagatgctgc catcaatcct gggaactcag
     4861 gtggaccact catcaatctg gacggcgaag tcattggcat caatcgtatg atcgtttctc
     4921 ccgcaggcgg ttctgtagga atcgggtttg cgattccaat caatgaagtg aagaaggtga
     4981 tcgcagacat caaagcgaat ggaactgtgg ttcgccctgc actcggggtt caaatcgaga
     5041 atctatctcc ggaaggaaag aagacgctcg gtgtggaaac aggagtggtg attcttggag
     5101 tggttcctgg atcgggtgcg gataaggcag gactcgagca aaaagatgtg atcacaaaag
     5161 tggacggcac catctacgac gacacggaac cacttcgctc ctacattctg acaaagagag
     5221 tcggagacaa agttaccctg gaaattgtgc gaaacaaagt ggtccagcag ctgcagatcg
     5281 tgctgggtaa gagacagtaa caggtttcaa tcgagagcgg cagcgatcgc aggaatcact
     5341 tgccgcatac gtttgagctc tgaaacttgg cgatcatgtc gtcgcaaaac tcgcgcacag
     5401 gcgctcgctt gcggtgactc cggcaaagaa tgtgagcgcc tttcgtagta aaattgttca
     5461 ggatgagagt tttctggcca atgcggctga tctgaaagct cgcacagaag atattcgcac
     5521 ccttgcttct tccattcagg aaggcggagg cgctcgcgcc aaaaagttgc acgaatctag
     5581 aggaaagctc tttgtgcggg atcgcattca gcttctgctc gaccctggat ctcccttcct
     5641 cgaaacgggg ctttttaccg ctcaccaggc gtatgacttc gatgtgccgt caggcggcat
     5701 tgtaactgga attggcagaa ttgaaaacac tctctgcatg attgttgcca atgatgcaac
     5761 agtcaaaggt ggcacatacc ttcctcttac agtcaagaag catcttcgcg cgcaggagat
     5821 tgctctggag aatcgtcttc cctgcattta cctggtggat tctggaggag cgttcctgcc
     5881 catgcaggat gaagtatttc cggacaagga acacttcgga cgaatcttct acaaccaggc
     5941 tgtgatgtca gcacagggaa ttccgcaaat tgcagtggtg atgggaagct gcactgcggg
     6001 aggcgcctat attccggcca tgtcggaaga gacaatcatt gtaagaggga atggaacgat
     6061 ctttctgggt ggtcctcctc ttgtaaaggc cgccacagga gaggatgtca ccgcagaaga
     6121 tctgggcgga gctcacgtgc acactcacaa gagtggtgtt gcagatcact ttgctcaaaa
     6181 tgatgagcac gctcttgaaa tcactcgcag aattgtaaaa catttaaact atcgtccggc
     6241 tgcttctgca tttgcggcac gcttcgctgc gcgctctcct atctacgctc ccgaagagat
     6301 atacggcatc attcaaaaag atctgcgcaa accatacgac attcgcgaga tcatggcgcg
     6361 catcgtagat gggtcagagt ttcatgaatt taaagagaat tacgcgacta cgattgtcac
     6421 tgcattcgct catgtgtttg gatttccagt tggaattgtt gcaaacaacg gagtgctgtt
     6481 ttcagaatca gccctgaagg ccgctcattt cattgagttg tgttcgcatc gtaagattcc
     6541 tcttctattt ctacagaaca tcaccggctt catggtcggg cgccagtatg agaatgcggg
     6601 cattgcgcgc gatggcgcca agatggtgac tgctgtatcc aatgcaaacg tgcccaagat
     6661 tacggtagtg gtcggcgggt cgtatggtgc cggtaactac ggcatgtgtg gtcgtgcgta
     6721 tggtcccagg tttctctgga tgtggcccaa tgctcgcatc tctgtgatgg gagcagaaca
     6781 ggcggccaac gtactactca ccgtaaagat ggaacagatc gcgcgtgagc ggggccaggg
     6841 tttaagccag gaagaacaga ttgaattcaa gaaacccatt ctagacgaat acggtcacaa
     6901 gtcccatgca ctctattcga gcgcacgtct ctgggacgat ggcatcattg atcctgcacg
     6961 gactcgcgaa gtagttggtc tttctcttgc agcatgtact ggcagtccag tcggcgaagc
     7021 accttcttcc ctctttcgca tgtaatccag ccaccgacgc cctgcccaca aagcaaaaca
     7081 aaacacttgc gcgcctgcgt gcgccttctt ctttttccag atgaagtatc ctcacctcct
     7141 ttctccgctc gatctgggtt tcactactct gcgaaatcgc gtattgatgg gatccatgca
     7201 caccggtctt gaggatatgc ctggcgggta tgaacgcatg gcggctttct atgctgagcg
     7261 agcggaaggc ggagttggtc tcattgttac gggcgggatt gcgcccaatg aagcgggaaa
     7321 agtcaatcgc accgggagcg tcctcgtcaa tgaggaggag gccaatcttc atatccccgt
     7381 gacgcgggcc gtacatgaac atggtggtaa aattgccatg cagattctgc acaccggcag
     7441 atacggatat cacgataaag cagtcggagc atctgaactg cgcgctccta tcaatgttgt
     7501 taaaccgcac aagctttcgc atcaagaaat tttgtccacg atagacgact ttgcgagatg
     7561 cacagaactc gctcgacacg caggctatga cggagtagag atcatgggct ccgaaggcta
     7621 cctgatcaat caattcaccg cgaagcgcac gaacaatcgc gatgacgact ggggaggatc
     7681 tcttgaaaac aggatgcgtt ttcctctcga gatcattcgc gctgtgcgaa agagggtagg
     7741 aaatgatttc atcatcgtgt atcgcctttc catgctggag ctggtggaag aaggcggaaa
     7801 tttggacgat gtgattcgcc ttgcgaagga agtggaagca gccggcgtga acattctaaa
     7861 cacaggcata ggctggcatg aagcgcgagt gccgaccatt gcaatgatgg ttcctagagc
     7921 cgcattcaca tgggtcacga agaatgttcg tcagcatgtg aagatacctt tgtgtacgtc
     7981 caatcggatc aatactccgg agattgcgga agccgttctt gcaaacgggg atgcggatat
     8041 ggtatccatg gccaggccgt tgcttgcgga ttctcatttt gtaaacaaag cagccgcgga
     8101 caaggcagac gaaatcaaca catgcattgc ctgcaatcag gcatgcctcg atcacatttt
     8161 tgttggaacg accgcgagct gcctcgtaaa tccaagagct tgcaatgaaa cagaactcaa
     8221 ctacattcct gcgactaaga agaagcgcat tgcagtgatt ggtgccgggc ctggtggtat
     8281 gtccgccgcg gcagtggccg cggaacgcgg acacaatgta acgttatacg acgctgctgg
     8341 cgacataggc ggtcagattc acattgcgcg cagaattccg ggcaaagagg aattcaatga
     8401 aactctcaga tactttcgca caatcttaaa aaagttcgga gttacacaga aactttctac
     8461 tcgagtttcc gctgcggatt tgatcgctga gaaatatgat gaggtcatcc tcgcgactgg
     8521 aatcaatccg cgcaaactca cgattccagg atcggatcga ccaaacgtgc tgagttatgt
     8581 ggatgtagtc ctgcacggca aagcagtcgg caagagagtg gccgtgattg gaggcggcgg
     8641 cattggcttt gatgtaacca tttacctcac tgaatccgaa tcttccgcag atcgcaagac
     8701 tgcatttctc gtagaatggg gaattgaccc atcgatcaaa gtttctggag ggctactccc
     8761 ggaacaaaag catcccgcgg cgccacgtca ggtgaccatg tttaaaagaa gcaaaggaaa
     8821 atttggcggg aacctgggca agacgacagg ttggattcac agacagactg tggaagatcg
     8881 ccatgtggaa cagatcagcg gcgtcaccta cgagaagatc gatgatcaag gtttgcatta
     8941 ttctctgaaa gggcagaacc acgttttgcc tgtggataca gttgtggttt gtgcgggtca
     9001 ggaaccaaac caggagttgc ttggaccttt gaagagcgca ggagtttccg tgcacgtcat
     9061 tggcggcgcc ctcgaagcag cagagctcga cgcgaagcgt gcgatcgacc agggcgccag
     9121 gcttgcggca tcactatgat ataaatcata gtgaatcgtg ttctttggtt tggccctggt
     9181 ttctgaatta tagttgagta agtttaacgt catgcgctca tgagggcatg aatggtggcg
     9241 aactcattgc tcagacattg caacttcacg gtgtgcgcgc cgtcttcact cttataggcg
     9301 ggcatatatc tccgattctc gtgagctgca agaaagctgg agtgagagta gtagatgttc
     9361 gtcacgaagt aaatacggtc tttgctgcag acgcctcagc cagattgact ggaatcccag
     9421 gcgttgcagt ggttacggca ggccccggcg tcaccaatac tctcactgcc ctcaagaatg
     9481 cacagatggc tcaatcgcct ctcgttctga ttggcggagc cacc
//
LOCUS       DAORFF010000068         9520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3368_length_9520_cov_3.187533, whole genome shotgun sequence.
ACCESSION   DAORFF010000068 DAORFF010000000
VERSION     DAORFF010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9520
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3368_length_9520_cov_3.187533"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PKK76_04370"
     CDS             <1..753
                     /locus_tag="PKK76_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNL67731.1"
                     /translation="IEVVFPDQRKERAKLIGRDETSDIAVIQIPGSDKLVPAVLGDSD
                     KVAPGQLAFALGSPFGLESTFTVGVISAKHRSIDNTKYTRIQTDTSINPGNSGGPLLN
                     VYGEVVGINQSIISPGGQGGSVGIGFAIPINEAKSVIEQLRKERRVIGRPTLGVQVGV
                     PTPNLRQFLKVSEEYGLVVRFVVPGSAAEDAGMKANDYLLKANDKELREPDELLEEVA
                     KAGIGGKIKLEFVRGGKKQSVTIAVGEDQSNQ"
     gene            750..1373
                     /locus_tag="PKK76_04375"
     CDS             750..1373
                     /locus_tag="PKK76_04375"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNL67732.1"
                     /translation="MIALEPNAEKWIRLESVDSTNSFLLREDLPSGTIVIADRQTQGR
                     GRHGRQWVSPEGSLILSGILCFDSEQISDDRLTLLPLLAGVALLRALQKMNPSVNLGI
                     KEPNDIIIDRNARKGKVAGILVESEIKGRERRVIVGVGVNATSAPETSDSVYQPAALF
                     DVSQGEGPFDRDECARLFVMELNARASQIESATPAFLGEVAQYRVGS"
     gene            1370..2155
                     /locus_tag="PKK76_04380"
     CDS             1370..2155
                     /locus_tag="PKK76_04380"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNL67733.1"
                     /translation="MKEYILAVDIGNTNTVFGLFENTADVEIQKDWRTVTRRDRTSDE
                     LGIFLLGFLQSSGIQSSQIKGFVYSSVVPSFNPIVERMAADYFSSVPLRVRYDNIPLK
                     IDYPRPEEIGADRLVNAVAADRMFPGNQIVVDLGTATTFCVVHEGTYLGGSIAPGLKL
                     SMEALFRNTAQLFPVEFSRPACGVISDSTRTAIQAGFFFGWVGLLRGILEEIHKTYPG
                     KKYHAIATGGLSAMIHREVPEIFAAVDPLLTLRGLKLIYQSQK"
     gene            2232..3746
                     /locus_tag="PKK76_04385"
     CDS             2232..3746
                     /locus_tag="PKK76_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNL67734.1"
                     /translation="MSELQKEAFGGDQSLHRALGPVNLMLLGIGAVIGAGIFVLTGTA
                     AAHNAGPGIVLSFVIAGTACGLAGLCYAEFASMLPISGSAYTYAYATLGELVAWIIGW
                     DLILEYSFASATVAIGWSGYVVSFLNDLGIVIPPELTQPPGRMLVLLKGKWVLLNDDL
                     IRTLLNLPAAQVITASEIQQALSGVQHVHSIMNLPAMLVIVFATVILVIGIRESARTN
                     AVIVVLKVAVVLIFVCVGAMYVKPGNWSPFIPENTGTWGEFGWSGVFRGAAVIFFAYI
                     GFDAVSTAAQESKNPQRDMPIGILGSLAICTILYILVSAVLTGLVSYKQLNVPDPIAV
                     GIDATGITWLAPLIKIAAIAGLTSVILVLLLGQPRIFYSMSRDGLLPEWFSKVHPKFK
                     TPYITTIATGVLVAVGAGLTPIDVVGHLVSIGTLFAFVIVCGAVLVLRVRHPEIKRTF
                     RVPFLPAVAVGGALFCGYLMASLPWETWERLIIWMVIGFVVYFLYGIRKSKLAK"
     gene            complement(3829..4980)
                     /locus_tag="PKK76_04390"
     CDS             complement(3829..4980)
                     /locus_tag="PKK76_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNL67735.1"
                     /translation="MSNKEFDFVVYGASGFTGRLVAEYLVQRGIRGWAMAGRSQGKLA
                     EVRDLVGAPKDTSLIEADASNPSSLSAMCDRAKIILTTVGPYQLYGSELVAACAASGT
                     DYLDLCGEPAWMRHMIDAHEAAAIKSGARIVFSAGFDSIPFDLGVWYLQQAAIAKFGS
                     PAPRVKGRVRKMAGGASGGTMASMKATLRASIKDAGILGLLKNPFALTPGFEGPAQPA
                     GIVPEYDSEAGVWAAPFMMAPINTKNVHRTNFLLKHPYGKDFVYDEMLLTTIGEAGRA
                     LVDAAAKAAFSGSGLKPGEGPSKEERESGFYDVIFIGQYPDGKRIQAAVKGDRDPGYG
                     STSKMISEATLTLLDLKTPGGVFTPGGIMAAPLIDRLVKYAGLTFEIES"
     gene            5074..5607
                     /locus_tag="PKK76_04395"
     CDS             5074..5607
                     /locus_tag="PKK76_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67736.1"
                     /translation="MHPPQLTMSRAAFLRGSIKLFALTSLTMSGVDCGPSSIPSLKGI
                     SAQEYRNMNAVGEVFLADSPFPDFDLGQALDDYVFGHPSPLDTKDIVRELAGAPSSYL
                     AAIVLDGSMTPLVKLSRADREARMLGWKKSDNIMKRGLYNILRQTTFFLLSSSPALPR
                     YAGYAIQGDLVPYGGGM"
     gene            5604..7193
                     /locus_tag="PKK76_04400"
     CDS             5604..7193
                     /locus_tag="PKK76_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNL67737.1"
                     /translation="MKKQIITPAEHKDIIKEFDLGRKWSLKADAVVIGSGAGGAVTAA
                     ELAKNGWKVVLIEEGSYFHPGIFSNDEFLSQARLYRDAGYVLTENQTASIVQGRTLGG
                     STTVNWQTSLYPPSYVTDEWRDRFGLPGYGRKEEISSYVEEVHKRLNVHEVPTKLINR
                     NNGVLMRGGRKLGLHPEVLKNNNNGCKGLGRCGLGCPINAKQSMFLTYIPDAIAAGAI
                     VVTNMRAVKISDGKTKRVTAEFTPDPYQASPEEIIEEMQIEAPVVIVSCGALEGPALL
                     QRSGFGNEMVGKNLKLHPTCTIFARFNSKIDMWHGPPQSVVIKDGHNQKGTGYGFWLE
                     VAPFRPTLTSAIMPFYGARQFRNIKDYTKFNAGIVLVRDGADGATKGKVEWSWGKRKV
                     HYEITRTDGTNMLLGLKMLAEVQAAAGATELIFPFTDMLEEFKVVPGSKFDWVLSRSF
                     EPGVLPIGSAHPHGSIQASSDPKTGAIDPNFELYGHRNIFVMDASWIPTGLSVNPQIT
                     TMSSALRAARKLAGEKSKRLD"
     gene            7203..7772
                     /locus_tag="PKK76_04405"
     CDS             7203..7772
                     /locus_tag="PKK76_04405"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HNL67738.1"
                     /translation="MNYLAHIYLSGEDEDLMIGNFIADSVRGNQLADFPEGVRKGIVL
                     HRKIDSYTDRHPVVTESKNRLRPEFGKWPPVIVDVFYDHFLAAGWEKYHAEPLPAYSK
                     RVYKILKKRKELLPERVRGFLPYMIYGNWLVGYSKLSGVEQALRGMSRRSRFNPEMHK
                     AVDVLKKQYEGFQDEFERFFLDLQAHVAE"
     gene            complement(7757..8281)
                     /locus_tag="PKK76_04410"
     CDS             complement(7757..8281)
                     /locus_tag="PKK76_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNL67739.1"
                     /translation="MNQHKPELKEFAALSSAALTESFNHGYAKIDARVLQLTDVQLDR
                     TFAKDSVAGEWSCRLLLGHLADAEIAFSHRMRRVAGEDKPALAAWDENEFITKGLYSA
                     LSASDSVDLIRSVRQWTGAWLRTLSQAQFDRTGQHPQRGEQSLHTILAYDTWHLEHHC
                     WFLDRKLEQIYSAT"
     gene            complement(8297..8929)
                     /locus_tag="PKK76_04415"
     CDS             complement(8297..8929)
                     /locus_tag="PKK76_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67740.1"
                     /translation="MQSDLEKAQAEIIRVQIERFHHFYADYFKREETHAMVEYFFDRI
                     YGLESDREWKRLALTTFQTVKHLVKEETRANIEHLMELNELTEELDADLARLLIDRHG
                     AEHRLSVNEYRDLYCSSSSPEKRRRHLELVLANMESFYQLAHRPINAYIMKPARFMTK
                     MLGIYPLFKTVEEGYYAVLPVTKELFESFVEEVSRREWEYLNSAFANAQT"
     gene            9038..>9520
                     /locus_tag="PKK76_04420"
     CDS             9038..>9520
                     /locus_tag="PKK76_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HNL67741.1"
                     /translation="MVSDQQIAEQAVKIRRLIVRMVHAAKCGHPGGPLGMADIFASLY
                     FDPEIFRHNPKRTDWPDRDRLILSNGHTCAVRYAAMCASGYFTEKEVMTFRKLGSPFQ
                     GHPSTKFLPAMENSSGSLGQGLSTAVGLALGRRVQKKNYNVFVCISDGECGEGMTWEA
                     A"
BASE COUNT         2275 a         2302 c         2483 g         2460 t
ORIGIN      
        1 atagaagttg tttttccgga tcaaagaaaa gagagagcaa agctcatagg aagagatgaa
       61 acaagcgaca tcgcagtcat tcaaattcca gggagcgaca aactggttcc tgcagttctt
      121 ggcgattcag acaaagtggc ccccggtcaa cttgcattcg cacttggatc tccgtttggc
      181 ctggaaagta cattcactgt gggtgtgatt tctgcgaagc acagaagcat agacaatacc
      241 aaatacactc gcatccagac agacacttcc atcaatccgg gaaactcggg cggaccactt
      301 cttaacgttt acggggaagt agttggaatc aatcagtcta tcatttctcc gggcggtcag
      361 ggcggaagcg ttggaattgg ttttgctatt ccaatcaatg aagccaaaag cgtgatcgaa
      421 cagttgcgca aagagcgtcg cgtcattgga agacctactc tgggagtgca ggtaggagtt
      481 cctacaccga acctgagaca gtttttgaaa gtaagcgagg aatatggact cgtagttcga
      541 tttgttgttc caggttctgc ggctgaggat gcgggaatga aagccaatga ctatctactg
      601 aaagcaaatg acaaagagct tcgcgagccg gacgagcttc tagaagaagt ggccaaagca
      661 ggaattggcg gaaagatcaa actcgaattc gttcgtggtg gcaagaagca gtccgtcacg
      721 attgccgttg gcgaggatca gtcgaatcaa tgatcgctct tgagccgaat gcagaaaaat
      781 ggattcggct cgaaagcgtt gattcaacca acagtttcct actgcgcgaa gatcttcctt
      841 ccggaacaat cgtcatcgca gatcggcaga ctcagggcag aggtagacat ggaaggcagt
      901 gggtttctcc cgagggctct ttgattttat ccggcattct ctgctttgat tcagaacaga
      961 tttcagatga tcgcctgacg ttgcttccat tgcttgccgg agtggctctg cttcgggctt
     1021 tgcagaaaat gaatccgtct gtgaatttag ggatcaagga accaaacgac atcatcattg
     1081 atagaaatgc acgaaaaggc aaggtagcgg gcattctggt agaatcagaa attaaaggcc
     1141 gcgaaaggcg agtgatcgtt ggcgttggag tcaatgcaac gagtgcaccg gaaacatctg
     1201 attcggtgta tcagccggca gcgctctttg atgtctcgca aggggaaggt ccattcgacc
     1261 gtgacgagtg cgctcgactt tttgtcatgg aactcaatgc aagggcatcg cagatagaaa
     1321 gtgccacacc tgcatttctg ggagaagtgg cacaatacag ggtaggttca tgaaagaata
     1381 cattctggct gtagatattg gaaatacgaa tacagttttt ggtttgttcg aaaatacagc
     1441 agacgttgaa atccagaaag actggaggac agtgacgcgt cgggatcgca cttcggatga
     1501 attaggtatt tttctattgg gttttcttca atcgtcagga attcaatctt cgcagatcaa
     1561 aggctttgtg tattcgtctg tggttccgtc tttcaatcca atcgtagaga gaatggccgc
     1621 tgactatttc agttccgttc cacttcgcgt gcgatacgac aacattccgc tcaagattga
     1681 ttatcccagg ccagaggaaa ttggagcaga cagactggta aacgcagtgg ctgcagacag
     1741 gatgttccct ggaaatcaga tcgttgtaga tctgggtacg gcgaccacct tctgtgtggt
     1801 tcacgaggga acgtatcttg gtggaagcat tgcgccgggg ctcaagcttt ctatggaagc
     1861 tttgtttcgc aacactgcgc agttgtttcc agttgagttt tcaagacctg catgcggtgt
     1921 gatttctgac tcaacgcgca cagccattca agcaggattc ttttttggct gggtgggact
     1981 tcttcgcggc attctggaag agattcacaa gacatatccg ggtaagaagt atcatgcgat
     2041 cgccacgggc gggctttctg caatgattca cagagaagtc cccgagattt ttgcagcggt
     2101 ggatccgctt ctgactctac ggggtttgaa gctgatctac cagtcacaga aatagattgc
     2161 atcctgacat gctcgcgcgc gaactgtgtg cgcgaggtat ccaattgagc gcactattta
     2221 gaagaaagcc aatgtcagaa ctccagaagg aggcattcgg cggagaccag tctcttcacc
     2281 gcgccctcgg tccagtcaac ctgatgctcc tcggaattgg cgctgtgatt ggtgccggga
     2341 tttttgttct caccggaacg gctgccgcac acaacgctgg ccctggaatc gtgctctcgt
     2401 ttgtgatcgc cggaaccgca tgcggcctcg caggtttgtg ctacgctgag tttgcatcca
     2461 tgcttccgat ttcaggaagc gcttacacgt atgcatacgc tactctgggt gaactggttg
     2521 cctggatcat tggctgggat ttgattttag agtactcatt cgcatctgca actgtggcaa
     2581 tcggctggtc cggctatgta gtaagttttc taaacgatct gggaattgtg attccaccgg
     2641 aattgactca accacctgga cgcatgctcg tcttgctcaa agggaagtgg gtgttactca
     2701 acgacgattt gattcgcact ctgctcaacc ttcctgcagc gcaggtgatc actgccagtg
     2761 agattcaaca ggctctcagt ggagtgcaac atgtgcacag catcatgaat cttcccgcca
     2821 tgcttgtgat tgtgtttgca actgtcattc tggtgatcgg gattcgcgaa tcggcgcgga
     2881 ccaatgcagt cattgtagta ctaaaagtgg cggtcgtttt gatcttcgtc tgcgtgggcg
     2941 cgatgtacgt aaagcccggg aactggagtc cattcattcc ggaaaacaca ggaacctggg
     3001 gcgaatttgg atggagtggt gtgtttcgcg gagctgctgt aattttcttc gcatacattg
     3061 gatttgatgc agtgtccact gccgcgcagg aatcaaagaa tccccagcgc gatatgccca
     3121 ttggcattct gggttctctt gccatttgca ccattctcta tatcctggtc tccgcagttc
     3181 ttactggcct ggtatcctac aaacagctaa acgttcctga tccaatcgct gtgggaattg
     3241 acgcgaccgg aatcacatgg cttgcgcctt tgatcaagat tgctgccatc gcaggtctta
     3301 cctctgtgat cctggtcctc cttcttggcc agcctcgcat tttctactct atgagccgcg
     3361 atggcctact tcctgaatgg tttagcaaag tgcatccaaa gttcaagact ccgtacatca
     3421 caacgattgc aacaggtgtt cttgtcgccg taggtgcagg tctcactccc attgatgtag
     3481 tggggcacct ggtgagcatt ggaacgttat tcgcctttgt gattgtgtgt ggcgcggttc
     3541 tggttctgcg agtgcgtcat cccgaaatca agcgcacatt ccgcgttccc tttcttcctg
     3601 cagtcgcggt gggaggagcg ctcttttgcg gctacctgat ggccagcctt ccctgggaga
     3661 cctgggaacg tttgattatc tggatggtga ttggatttgt agtttatttt ctatacggca
     3721 ttcgtaagag caaactggca aaatgaaaat agacccgccg gccctgggcc gcgcgggttt
     3781 ccatgatcaa aggatggtgg agaccgttcc tatcgtctcg ctttttttct aggattcaat
     3841 ttcaaaagtg agtcctgcgt atttcaccag gcgatcaatc aaaggagccg ccataattcc
     3901 acctggagtg aacactcctc caggagtttt caaatccagg agagtcagag tcgcttctga
     3961 aatcattttg cttgtagatc catagcctgg atcgcgatct cctttgactg ctgcctggat
     4021 tctctttccg tctggatact gtccaatgaa gatgacatca tagaaaccgc tttcgcgctc
     4081 ttccttagat ggaccttctc ctggcttcag accgcttccg gagaacgcag cttttgcagc
     4141 cgcgtccacg agggcacgcc ccgcttcgcc gatcgtagtt aagagcattt cgtcatacac
     4201 gaagtctttt ccatacggat gttttaacag gaagttcgtg cgatgaacgt tcttcgtatt
     4261 gatcggcgcc atcatgaatg gagccgccca cactcctgct tctgaatcgt actcaggaac
     4321 gattccagcc ggctgtgcag gtccttcaaa acctggagtc agagcaaatg gatttttcaa
     4381 cagtcccaga atgcctgcgt ctttgatcga tgcacgcaga gtcgctttca tgctggccat
     4441 ggttccgccg gaagctccgc ccgccatctt gcgcactctt cctttgactc tgggtgcagg
     4501 ggatccaaac ttcgcaatgg cggcttgctg caaataccat acgccaagat caaatggaat
     4561 ggaatcaaat cccgctgaaa aaacaatgcg cgctccgctc ttgattgcgg ctgcttcatg
     4621 agcgtcgatc atatggcgca tccacgccgg ttccccgcat aaatccagat aatcggttcc
     4681 acttgcagca caggcggcca caagttctga gccatacagc tggtaaggac ctacagttgt
     4741 aagaatgatc ttcgcgcgat cgcacatggc gctcaaagag gatggattgg atgcatctgc
     4801 ttctatgaga gatgtatctt ttggggcgcc cacaaggtct ctgacttcag ccagtttgcc
     4861 ctggcttctt cccgccatgg cccagccgcg aatgcctctt tgcaccagat actcggccac
     4921 gagcctgccg gtgaaaccgc ttgctccgta cactacaaaa tcaaattcct tgttgctcat
     4981 aaccaccttt aggctcggtc gaataaactg tcaaagtcga ttctaaccta tgttcgagtt
     5041 tgacgatttt cgggcccgcc agcagaaacg accatgcatc ctccccagct cacgatgtcc
     5101 cgggccgctt tcttgcgcgg ttcgatcaag ctgtttgctc ttacatctct gactatgtca
     5161 ggagtagact gcggcccctc cagcatccct tctctgaaag gaatcagcgc acaggaatat
     5221 cgcaacatga acgcggtagg tgaagtgttc cttgcggatt caccttttcc tgattttgat
     5281 ctgggtcagg cgctcgacga ctatgtgttc ggtcatcctt cgcctctgga tacaaaggac
     5341 attgtgcgcg aactggcagg agctccttct tcttatctgg ccgccatcgt gctggatgga
     5401 tctatgactc cgctggttaa actatctcgc gcggacaggg aagcaagaat gctcggctgg
     5461 aagaagtcgg acaacatcat gaagagaggt ctttataata tactgaggca aacaacgttc
     5521 tttctacttt catccagtcc ggcgcttccg cgttacgccg gttatgcgat ccagggagat
     5581 ctggtacctt acggaggtgg aatgtgaaaa agcaaatcat cactccagca gaacacaaag
     5641 acatcatcaa agaatttgat ctcgggcgaa agtggagtct gaaagcagac gcagtcgtca
     5701 taggttctgg agcgggcggt gctgtgaccg cagcagaact ggcgaagaac ggttggaagg
     5761 tggtcctgat cgaagaaggg agttactttc atccgggaat tttttcaaac gacgaatttt
     5821 tgtctcaggc aaggctctat cgcgatgcag gctacgtgct gactgaaaac cagacggcaa
     5881 gcattgtgca gggcagaact ctgggtggat ctaccacagt caactggcag acttctctgt
     5941 atcctccatc gtatgtaaca gacgaatggc gggacaggtt tggacttcca ggttacggaa
     6001 gaaaagaaga gatttcttcg tacgtagaag aggtccacaa aagactcaac gttcacgaag
     6061 tccccacgaa attgatcaac agaaacaatg gagtgctcat gcgcggtggt cgcaagcttg
     6121 gcctccatcc tgaagtgctc aagaacaaca acaacggttg caaaggtctt ggacgatgcg
     6181 gtctgggctg tccgatcaac gcaaagcagt ccatgttttt gacctacatt ccggatgcga
     6241 ttgccgcggg cgccattgta gtgaccaaca tgcgcgctgt taaaatcagc gatggcaaaa
     6301 caaaacgagt cacggcagaa ttcactccag atccatacca ggcttcgcct gaagagatca
     6361 tcgaagagat gcaaattgaa gcgccggtgg tgattgtaag ctgtggcgcg ctcgaaggtc
     6421 cggcactttt gcagagatcc ggttttggaa atgaaatggt tggtaaaaat ttgaaactcc
     6481 atccaacctg cacgatcttc gcacgcttca attccaaaat cgacatgtgg cacggtccac
     6541 cgcaatctgt agtgatcaag gatgggcaca accagaaagg aactggctat ggtttctggc
     6601 tggaggtggc gcccttccgg ccaactctaa cttctgcgat catgcctttt tatggagcca
     6661 gacagtttag aaatattaag gattacacga agttcaatgc gggcattgtt ctggttcgcg
     6721 atggagcaga cggagccaca aagggcaaag tggaatggag ctggggaaaa agaaaagttc
     6781 actacgaaat cacgcgaaca gatggaacca acatgcttct gggcctcaag atgcttgcgg
     6841 aagtccaggc ggcggcgggt gcaacagaac tgatctttcc gttcacggac atgctagaag
     6901 aattcaaggt ggtgcctggt tcaaagttcg actgggttct ttcgcggagt tttgagcctg
     6961 gagttttgcc cattggttct gctcatccgc acggatcgat tcaggcttcc agtgacccaa
     7021 aaacaggagc cattgatccg aattttgagc tctatggaca tagaaacatc ttcgtgatgg
     7081 acgcttcctg gattccaacc ggattgtctg tgaatccgca aatcacgacc atgagttctg
     7141 cgcttcgtgc agctcgaaag cttgctggag aaaagtcgaa gcgtctggat tgatcagacg
     7201 gaatgaatta ccttgcacat atctacctgt ccggagagga cgaggatttg atgatcggca
     7261 attttattgc agattctgtg cgaggcaatc agctcgctga ttttccagaa ggcgttagaa
     7321 aaggaattgt tcttcaccgt aagattgatt cttatacgga tcgtcatccg gtcgtgacag
     7381 agagcaagaa tcgcctgcgc cctgagttcg ggaagtggcc acctgtgatt gttgatgttt
     7441 tttatgatca ttttctggcc gcgggctggg aaaaatatca tgctgagcct ctgcccgcgt
     7501 actctaaacg ggtatacaaa atactaaaga aaagaaagga actgcttcca gaacgagtgc
     7561 gtggattctt gccgtacatg atttacggaa actggttggt agggtacagc aaactctcag
     7621 gagtggagca ggcgcttcga ggaatgtcca gaagatccag gttcaatcct gagatgcata
     7681 aagcagtgga tgtcttgaaa aagcaatacg aggggtttca ggatgaattc gaaagattct
     7741 tcctggatct ccaggctcac gttgccgagt aaatctgttc taactttcga tccagaaacc
     7801 agcaatggtg ttccaggtgc catgtatcat aggcaaggat cgtgtgcagc gattgttctc
     7861 ctctttgtgg atgttgaccc gttctgtcga actgtgcttg tgaaagagtc ctcagccagg
     7921 ctccggtcca ttgtcgcacg cttcggatca aatccacaga atcagaagcg gaaagggcag
     7981 agtatagacc cttcgtgatg aattcatttt cgtcccatgc tgcgagtgcg ggtttgtctt
     8041 ctccggccac ccgtctcatt ctatggctga atgcaatttc agcatctgcc aggtgtccga
     8101 gtaagagtct acatgaccat tccccggcga ccgaatcttt tgcgaacgtg cgatcaagct
     8161 gcacgtccgt cagttggagc actcgcgcat ctattttcgc atacccatga ttaaatgatt
     8221 ccgtaagtgc ggcgctggac agggctgcaa attctttgag ttccggtttg tgctgattca
     8281 tctatcgatt cctttctcag gtctgcgcat ttgcgaatgc gctgttcaga tactcccatt
     8341 cgcgtcgcga tacttcttcc acgaatgatt cgaacaattc ttttgtgacc ggaagaactg
     8401 catagtatcc ttcctccaca gttttgaaca gaggataaat gcccagcatc ttagtcatga
     8461 accttgcagg cttcatgatg tatgcattga tgggccgatg tgcgagctga taaaatgatt
     8521 ccatgtttgc cagtacaagt tcgaggtgcc ttcgccgttt ttctggagaa ctggaagagc
     8581 agtacaaatc tctatattca ttcacggaca atctgtgttc tgcgccatgg cgatcgatca
     8641 gcagtctggc cagatctgcg tcgagttcct ctgtgagttc gttgagttcc atcagatgct
     8701 ctatgttcgc acgagtttct tctttgacca ggtgcttgac tgtctggaat gtagtgagtg
     8761 caagacgctt ccattctcgg tcgctttcca ggccatagat cctgtcgaag aaatattcga
     8821 ccatcgcatg cgtctcttcg cgcttgaagt agtctgcgta gaagtggtgg aacctttcta
     8881 tttgaactcg gatgatttca gcctgcgcct tctccagatc ggactgcatg cctgcaaaac
     8941 tggcaggtag cgcgagcctg tcatgcctct ttcgagggca tacttgaggt tggcgaaatc
     9001 gtttacaggc taacggccca ggattttcag tctaatcatg gtttcggatc agcagattgc
     9061 agagcaggca gtcaaaattc gcagattgat tgtgcgcatg gttcatgcgg ccaaatgcgg
     9121 gcatccaggt gggcccctgg gaatggcgga tattttcgcc tcactctatt ttgatccaga
     9181 aatcttccgt cacaatccaa agcgcacgga ctggccggat cgcgatcgtt tgattctttc
     9241 caatggtcac acatgtgccg ttcgctatgc ggccatgtgc gcgtccggct atttcacgga
     9301 aaaagaagtc atgacttttc gcaagctcgg ctctcctttc cagggccatc catctaccaa
     9361 gtttctgccg gccatggaga attcctctgg ctctcttggc cagggacttt cgacggcggt
     9421 gggtcttgcc ctgggtcggc gtgttcaaaa aaagaactac aatgtattcg tctgcatttc
     9481 ggacggagaa tgcggagaag gtatgacctg ggaagcggcc
//
LOCUS       DAORFF010000069         9462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3403_length_9462_cov_3.431487, whole genome shotgun sequence.
ACCESSION   DAORFF010000069 DAORFF010000000
VERSION     DAORFF010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3403_length_9462_cov_3.431487"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..912
                     /locus_tag="PKK76_04425"
     CDS             184..912
                     /locus_tag="PKK76_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNL67742.1"
                     /translation="MIEPDKNQNAIVARELPPLAAGLVHEVRNPLAAIHLHLQLLESY
                     IGQVRDPELREKMQGKARVIKTEIMNLNQTLQDFLRMLREERVEKQIGDLNTAVREVI
                     ELLEPQARQEHIDLAFRPGELGSNTVFEPTFVKQILVNLILNSVQAFEKAKQQADKRI
                     FVETGMEGKNAFFRVRDNGPGISPEVQARIFEPFFSTKEEGSGLGLSLVQKMTGEMGG
                     DVRVSSVPDEGAEFTIVLGRTGLQ"
     gene            934..1377
                     /locus_tag="PKK76_04430"
     CDS             934..1377
                     /locus_tag="PKK76_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HNL67743.1"
                     /translation="MTDVWQLHYRSLENMYHKNAKINEFFHPELKIARMEAEVKIPVV
                     PDFFHAGGSVHGSVYFKALDDAAFFAANSIVRDVFVLTLTFQIQLTRAVRDGIMTAKG
                     RVLQNLGNQIMAESILYDERGKETARGSGVFVKSKLPLSPEIGYV"
     gene            1377..2744
                     /locus_tag="PKK76_04435"
     CDS             1377..2744
                     /locus_tag="PKK76_04435"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNL67744.1"
                     /translation="MPNILIIEDDPAQRSALEEFLTDYLGKKGNVFAAEDLETARTVF
                     RDRKIQLIVTDLMLPDGTGIEFVKEIKDSKIPVLLLTGQPSIETAIEAIRAGASDYLI
                     KPVDLSHLQVKITSLLENSELKEENRILKARLQDRFSAKNIVGNAQIMKPILDRIRQV
                     APTDVTVLLEGESGTGKELFANLLHENSPRADKPFIKVNCGALTKTILESELFGAVKG
                     AYTGSDRDRAGYFESANGGTIFLDEIGEMDLESQVRLLRVIEEREVVRVGSSKPAKVD
                     VRVIAATNRSLQKEVEAGKFREDLYYRLAVIRIELPALRMRKEDIPILFNHFVTGFNE
                     KYRKSVNALSPELNHFFQQYDWPGNIRQLRNVLEGMVVLAQDNSLTLADLPDELAHPT
                     RRTADKKLMDSVLPGIAMDDYEKAIILKNLSYTGGNREKAAKLLGLSERTLYRKIKDY
                     GMEND"
     gene            2737..3498
                     /gene="recO"
                     /locus_tag="PKK76_04440"
     CDS             2737..3498
                     /gene="recO"
                     /locus_tag="PKK76_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNL67745.1"
                     /translation="MIESARGIVLRRKKDRESDAVITLLCEDGRLLRARFHGILQSKT
                     RSNLTAEPGSHVELVLQSKDASIASVKEGTLLERNDVWKSGYRPLSLLSSVLEFAEGM
                     ASACDSDDLYRLIAGGMEEARVHARLFEEPESILFFTAIRIRGLTIAGLTGDLSYCAS
                     CGERLGKQARWSVPEISFLCNKCSEETTEADARAAHTLARMAAEGFSSLLSVRQGDNG
                     YIDHLKRVEQLLAICIRHALPFPSPAADALTGGPA"
     gene            3495..3692
                     /locus_tag="PKK76_04445"
     CDS             3495..3692
                     /locus_tag="PKK76_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67746.1"
                     /translation="MKRFFQFLMPALALLLLGSALLSRVFPGATEALVTTKKVLVARL
                     ADLDPFLKTQAEGHGSKKEAR"
     gene            3667..5721
                     /locus_tag="PKK76_04450"
     CDS             3667..5721
                     /locus_tag="PKK76_04450"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNL67747.1"
                     /translation="MAAKKKPGKAAKKTGKKSAKKTGAKLSAQQPAPRPRGRPPGTKS
                     EKKRGRPPGSEKQASEARDVPRKKRDQQPFVTLKKMVQEAIVAALPEYLKESRIPIEY
                     SREEKFGDYAATAAMDKDFRETLSKRNPACKNPREAGAYLLKQLRASEESSTIFANVE
                     IAGPGFLNISISPSVLATFAARAVKNPETYGRTESAEKRKIIFEFVSANPTGPLNIVS
                     ARAAALGDSCCNLLSWAGESVFREYYVNDYGNQIQLLGQSCFLRALEETGIPVKISEK
                     TDQGPVYPEGPGLPFPSEGYHGDIIKDIVQEIRKRKSVLIGPRVVEHARQIAGSPDVP
                     LDFISRHPAMQELSDKLGEAAVSVFLDTQQRDLERFRVKFDAFYRESSLHKQNKVLSA
                     RNFLAEYVRTEDGKQIFESKKFGDDLDRVIIREDGRPTYLLADIAYHKTKMDRGFDEI
                     INIWGPDHHGYIKRLAGALVAMGYSAEKFKVLLAQQVNLLEHGQPVVMSKRAGKFVTM
                     DSLMNEIPIDVIRYFFVMRSFEAHLDFDFAEAADTSEKNPYYYVAYAHARICSIQRKA
                     LEKGISIPVEAREMEECMRTLEWTADRRRLLYLVARFPEEVQDAARVLEPHRLINFLY
                     HLANALSRFYAPKENRVIDQDERTARGLLVLLSAVATCLKNGLAILGMTAPDRMTREE
                     SA"
     gene            complement(5718..6515)
                     /locus_tag="PKK76_04455"
     CDS             complement(5718..6515)
                     /locus_tag="PKK76_04455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNL67748.1"
                     /translation="MKNKTVLITGSTDGIGVQTGLELAKQGARILIHGRNPDRAAKVV
                     SQIESAGGQAQSLIADLSSMADVKKLAAAANAAGPIHVLINNAGVFMKEKKLTVDGFE
                     TTFAVNHLAVFLLTNELLPSMPDGSRIITLSSIAHKNGKIDFDNLQSEKSFDGYAAYA
                     MSKLCNVVFSYELARRGASRGILSNAVHPGVIQTKLLKEGFNMDSAGSLEEGAATSIY
                     LASSPEVQNVTGKYFVSSRESVSTAASRDEALQRKLWDVSEQLLSRF"
     gene            complement(6734..7558)
                     /locus_tag="PKK76_04460"
     CDS             complement(6734..7558)
                     /locus_tag="PKK76_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL67749.1"
                     /translation="MLKIPRPDAQQLVGLHGFADTGETFQFVQAPLANRFELLFPDMR
                     GHGDSSRLRESYYGAHSYFGDLIRFTGTVKKPYVLMGHSMGAAMAARFAGLYPDEISH
                     LILLEGFSGISSEEEEIRRLRSFGDNLRKKRPEKVRILKTKREAELILGTVHEGLPPE
                     RISALAEYLAAPVEGGFVWKQDPEVKTGGIPIPFSPAFSRALWKRITCPVLFIYGERS
                     HLLPGKRTNSSGSLDEILSHFQNLETHSLDSGHNPHHEKPEEVMQLLEAFFQKHPG"
     gene            complement(7599..8150)
                     /locus_tag="PKK76_04465"
     CDS             complement(7599..8150)
                     /locus_tag="PKK76_04465"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNL67750.1"
                     /translation="MKWILTNEEDLLSTVPFTVQRLSLVDRVRGPLHDFHRLRAPDWA
                     NILPITREGEALLIRQPRAGAMELILESPGGAVDAAETDPLVAAARELEEETGYTSER
                     MTHIASFNPNPALFSNKIHFFLAEGCFINPNREHFPDSSEDIEIVLVPLDELEERVAR
                     GEINHALAALGIMLSLRRLKSGN"
     gene            complement(8150..9289)
                     /locus_tag="PKK76_04470"
     CDS             complement(8150..9289)
                     /locus_tag="PKK76_04470"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNL67751.1"
                     /translation="MKFAAFTILAFFVSISAIQAEQIIMKTGQVYNGRIVNQTRNEVI
                     LDTAAGRVVLRKIDIRRITYGPTPEDQTRDARKREEQKKAEEDKKKEDQKLAEEKKKA
                     EEDKNRADQKLADEKRQAEEKQKAEELKKIEDSKKSEEQKAADADRLKKEEEARKAAE
                     EKKQAELLKQQEELKKSEEKKTDELRVTEEQIRERVRQEVQLELQKQEQRRREEERRR
                     LEEEAKRQSQPTRSGAFFRSLVLPGWGQLYQGRRIAGFAYMGGVLGAAAVSNYQDQIY
                     KRRLGEYHDITNTFLFSTPFVTRLLGYAQDDYQASTVFFLGTDQTQKAYNGMAKAARR
                     GRWARGVALGLYAGNLVDVLIFAPRASVGVTPSQDGFNLAFHYQF"
BASE COUNT         2440 a         2411 c         2368 g         2243 t
ORIGIN      
        1 atgatccttc gcgcgtttat ttgaaaactc agggagacgt acctcgattg ctcatcacaa
       61 ccaaagacaa tctacaacgc gcatcgcatg tttcgccaga agacgtgcat ttcttgttgt
      121 gcgaggtagt cgatgtgcag gggcccggac agggagatct ccgcaggcaa gagaaatagc
      181 tagatgatag aaccggacaa aaaccaaaat gcaattgtag cgcgagagct tcctcctctg
      241 gcagccggcc tggttcatga agttagaaat ccgctcgcag ccatccacct gcatctgcag
      301 cttctggagt cctacattgg ccaggtccgt gatcctgaac tccgcgagaa aatgcagggg
      361 aaagcacgcg tcatcaaaac agagatcatg aatctcaatc agactctaca ggatttcctc
      421 cgcatgctgc gcgaagagag agtggaaaaa caaatcggag atctcaacac tgcagtgcgc
      481 gaagtcatcg aactgctcga accgcaggca aggcaggagc acattgatct tgcttttcgt
      541 cccggcgaac tagggtccaa caccgtattt gaacccacat tcgtaaaaca aatacttgta
      601 aatctcatac taaattcagt gcaggctttt gaaaaagcaa agcagcaagc agacaaacgc
      661 attttcgtag aaacaggcat ggaaggaaag aatgcattct ttcgcgtacg ggacaatggg
      721 cccgggattt ctccggaagt gcaggccagg atttttgaac cgttcttttc cacaaaggaa
      781 gaaggaagcg gacttggact ttctctggtc cagaagatga caggagaaat gggcggggac
      841 gttcgagtca gctctgttcc agatgaggga gcggaattca cgatcgtact gggcagaaca
      901 ggattgcagt aaattccgag aagaagagat caaatgaccg acgtctggca gctccactat
      961 cgcagtctcg agaatatgta ccacaagaat gcgaagatca acgaattttt tcacccagag
     1021 ctcaagatcg cacgcatgga agcagaagtc aaaatcccgg tggtgcctga tttctttcac
     1081 gcaggtggtt ctgtgcacgg cagcgtttat ttcaaggcac tcgacgatgc tgcattcttt
     1141 gccgccaatt ccatagtgcg cgatgtgttt gtactcacgt tgacttttca gattcaatta
     1201 acaagagctg ttagagatgg aatcatgact gcgaaaggtc gcgtgcttca gaatcttgga
     1261 aatcaaatca tggcagaatc cattctgtac gatgaacgag gaaaagaaac ggcacgcggc
     1321 agcggcgtct ttgtcaaaag caaacttcca ctgtcccctg aaattggtta cgtctgatgc
     1381 ccaacattct gatcatagaa gacgatccgg cgcagcgctc agccctggaa gaatttctca
     1441 cagactacct gggcaagaag ggaaacgtat ttgcagccga agatctcgaa acagctcgca
     1501 ctgtatttcg ggacagaaag attcaattga ttgtcaccga cctgatgctg cccgatggaa
     1561 ctggaatcga attcgtaaag gaaatcaaag attccaaaat cccagtgctt ttactgacag
     1621 gacagccttc gattgaaaca gccatcgagg cgattcgcgc gggggcatcc gactatctga
     1681 tcaaacctgt ggacttgagc catcttcagg tcaagatcac ttccctcctt gaaaattcag
     1741 aactgaaaga agaaaacaga attctgaaag ccagactgca ggatcgcttc agcgcgaaaa
     1801 acattgtagg gaatgctcag atcatgaagc caattctcga cagaatccgt caggtggctc
     1861 cgacagacgt tacagttctc ctggaaggag aaagtggtac aggaaaagaa ctcttcgcga
     1921 acttgctgca tgagaactcc cctcgcgcgg acaagccatt catcaaggtg aactgtggcg
     1981 cgttgacaaa gacaattcta gaaagcgaat tgtttggagc tgtgaaaggt gcttacacgg
     2041 gaagcgatcg ggatcgagcc ggctactttg aatccgcgaa tggtgggact atttttttgg
     2101 atgaaattgg agagatggac ctggaaagcc aggtgcgtct gctgcgagtg attgaagaac
     2161 gagaagtggt tcgagttgga tcttcaaaac cggcaaaggt ggatgtgcgc gtgattgcgg
     2221 ccaccaatcg ttctttgcaa aaggaagtgg aagcaggaaa attcagagag gacctctact
     2281 acagactcgc tgtgattcga atcgaacttc cggcccttcg catgcgaaaa gaagacattc
     2341 ctatcctgtt caatcatttt gtgacagggt tcaacgaaaa gtatagaaaa tcggtgaatg
     2401 cactttcgcc agaactcaat cacttcttcc agcaatatga ttggcccgga aacatccggc
     2461 aattgcgcaa tgttctggag ggcatggtcg tgctggctca agacaattct ctcacgcttg
     2521 cagaccttcc agacgaactc gctcatccaa ctcgcaggac cgcggacaag aagttgatgg
     2581 attctgtttt gccaggaatt gccatggacg actacgaaaa agcaatcatc ctcaaaaacc
     2641 tttcttacac cggcggaaac agagagaaag ccgcaaagtt gctgggcctt tccgaacgca
     2701 cattgtatcg caaaatcaaa gactacggta tggagaatga ttgagtcggc tcgcggcatc
     2761 gtacttcgca gaaagaaaga ccgagagtct gatgctgtca tcaccttact atgcgaagac
     2821 ggaaggctac tccgtgcacg ctttcacgga atcttacagt ccaaaaccag aagcaacctg
     2881 acggcagagc ctggatcgca tgtagaactg gttctccagt ccaaagacgc atccatcgcc
     2941 agtgtgaaag aaggaactct cctggagaga aacgacgtct ggaagagcgg ctatcgtccg
     3001 ctcagcctgc tttcatcggt gctggaattc gcagaaggta tggcaagcgc gtgcgattcg
     3061 gatgatctct atagactcat cgcaggcggg atggaggaag cgcgagtgca cgcaagattg
     3121 ttcgaagagc cagaatcgat cttattcttc acggcaattc gaattcgcgg cctcacgatt
     3181 gcaggtttaa ccggggatct atcctactgc gcatcgtgcg gagaaaggct cggaaaacag
     3241 gcacgctggt ccgttccgga aatttccttt ctctgcaaca agtgcagcga ggagactacg
     3301 gaagcggatg caagggccgc acacactctg gcgcgcatgg ctgcggaagg gttttcatct
     3361 cttttgtccg tgcgccaggg agacaatggc tacatcgatc atctgaaacg agtggaacag
     3421 ttgcttgcca tttgcattcg ccatgctctt cctttcccga gccccgcagc cgatgctttg
     3481 actggaggcc ctgcatgaag cgattctttc agttccttat gcctgccctt gcactcttgc
     3541 ttcttggctc ggctctctta tccagggttt tcccgggcgc cacggaggcg cttgtgacca
     3601 ccaaaaaagt gctggttgcc agactggccg atctcgatcc tttcctgaaa actcaggcag
     3661 agggccatgg cagcaaaaaa gaagcccggt aaggcagcga aaaagacggg caagaaatcg
     3721 gccaagaaaa ccggcgccaa actttccgca caacaaccgg cccctcgtcc tcgcggcaga
     3781 ccaccgggca ccaaatcaga gaagaaaaga gggagacctc ccggatcaga gaagcaagcc
     3841 tcggaagcgc gagacgttcc gcgaaaaaag cgcgatcagc aaccattcgt cactctgaag
     3901 aaaatggtgc aggaagccat cgtagcggct ctccccgagt atctgaaaga atctcgcatc
     3961 cccattgaat attcccgcga agaaaagttc ggagactacg ccgccacagc cgcgatggac
     4021 aaagacttcc gcgaaacatt gagtaaacgc aatcctgcgt gcaagaatcc gcgcgaagcc
     4081 ggtgcgtatt tactcaaaca gttacgagcc agtgaagaaa gttctaccat cttcgcgaat
     4141 gtagagatcg ccggaccggg ttttctaaat attagcatct ccccttccgt cctcgctacg
     4201 tttgccgcgc gagcagtgaa aaatccggaa acgtacggca gaacagaatc tgcggaaaaa
     4261 agaaagatca tttttgaatt cgtttctgca aatccgaccg ggccactgaa tatagtttca
     4321 gcaagagccg cagccctggg tgattcctgt tgcaatctac tttcctgggc gggggaatct
     4381 gtctttcgcg aatactatgt aaacgactac ggaaatcaaa ttcaacttct tggccagagc
     4441 tgttttcttc gcgctctcga agagacagga atcccggtaa aaatttcaga aaaaacagat
     4501 cagggacctg tgtatccaga gggtcctgga ctgcctttcc catcagaagg gtatcacggc
     4561 gacatcatca aggacattgt ccaggaaatt cgcaaacgga aatcggtgtt gatcgggccg
     4621 cgcgtagtag aacatgcgcg acagatcgcc gggtcaccgg atgttccact tgattttatt
     4681 tccaggcatc cggccatgca ggagctttcg gacaaactcg gggaagcagc cgtcagtgta
     4741 tttctggata ctcaacagag agatctggaa cgctttcgcg taaaattcga cgccttctac
     4801 cgcgagtcca gtcttcataa acaaaataaa gttctttctg cgcgcaattt tctggcggaa
     4861 tacgttcgca cagaagatgg caagcagatc tttgaaagca agaagtttgg agacgatctg
     4921 gatcgagtga tcattcgcga ggatggccgg ccaacgtact tgcttgcgga catcgcatat
     4981 cacaaaacaa aaatggaccg tggctttgat gaaatcatca acatctgggg gcccgatcat
     5041 cacgggtaca tcaaacgtct cgccggcgct cttgtagcca tgggatactc cgcagaaaaa
     5101 ttcaaggtac ttctggcgca acaggtaaat ttactggagc acggccagcc agttgtcatg
     5161 agcaagagag cgggtaagtt tgtgaccatg gacagcctca tgaatgaaat cccaatcgac
     5221 gtcattcgct acttcttcgt gatgagatct tttgaggctc atctggattt tgatttcgca
     5281 gaggccgcgg acacttctga aaagaatcct tattattacg tagcatacgc gcacgctcgg
     5341 atctgttcta tccagagaaa agccctggaa aaagggatct ccattccggt ggaagctcgc
     5401 gagatggaag aatgcatgcg gactcttgaa tggacagcag acagaagaag actactctat
     5461 ctggttgcca ggttcccgga agaagtgcaa gacgcagcca gagtacttga accgcacaga
     5521 ctcataaact ttctttatca tctggccaat gctctttctc gattctatgc acccaaagaa
     5581 aacagggtca ttgatcaaga tgagcgcaca gccagaggac tgcttgtact tctgagtgca
     5641 gtggccacct gcctcaagaa tggcctggcc attctgggca tgacggcacc ggatcgcatg
     5701 acccgcgaag aatcagccta gaagcgcgaa agcaattgct ctgaaacatc ccagagtttg
     5761 cgctgcaatg cctcatcgcg agaggcggca gtacttacgc tttcgcgcga gcttacgaag
     5821 tattttccag tcacattctg aacttcagga gaactggcga gataaatcga tgtagccgca
     5881 ccctcttcca gagacccggc agagtccatg ttgaaacctt ctttgaggag tttggtctga
     5941 atgactcctg gatgcactgc gttagacagg atgccacgcg aagcgccgcg acgagcgagc
     6001 tcgtaggaaa aaaccacatt gcacagcttg ctcatcgcgt atgcggcgta accatcgaac
     6061 gatttctcag actgcagatt atcaaaatcg attttgccat tcttgtgggc gatggagctg
     6121 agagtaatga tgcgcgatcc atccggcatg gaaggaagaa gctcattcgt gagcaggaac
     6181 acagccagat gattgaccgc gaacgtagtt tcaaatccat ccacagtaag ctttttttcc
     6241 ttcataaaga cgcccgcgtt gttgatgagc acgtggatgg gcccggccgc attggccgcc
     6301 gccgccagct tctttacgtc cgccatggaa gacaaatcag cgatgaggct ttgtgcctga
     6361 ccgcccgcag attcaatttg agaaacaact tttgccgcgc gatccgggtt tctgccatga
     6421 atgagaatgc gagccccctg tttcgccagt tcgagccctg tctgaacgcc gattccatct
     6481 gttgagcctg taatgagtac ggttttgttt ttcaccggga gaatataaca aatgttctct
     6541 gtaaggcact cgatttttct atggtcagga gaacgagaac atcgcccggg cgacagagaa
     6601 tgggcgcgaa tccaggtgag gatttctcgc cctacaaatg gcgatttcgc gccgcgattc
     6661 tatatttttt cgaatagcag ttcgcgagaa atcttcaacg aatgcgcggc ccgataggcg
     6721 acattgcggt tcttcaccca ggatgttttt gaaaaaaagc ttcgagcaat tgcatcactt
     6781 cttccggttt ctcgtgatgc gggttgtggc ctgagtccag agaatgtgtt tccaggtttt
     6841 gaaaatggct caggatttca tccagagatc cggatgagtt cgtgcgcttg ccgggcaaaa
     6901 gatgactgcg ctcgccatag atgaacaata caggacaagt gattctcttc cagagggcac
     6961 gactgaatgc cggactgaac ggaatgggaa tgccgcctgt tttcacttcc ggatcttgct
     7021 tccagacgaa tccgccttcc acgggtgctg ccagatattc ggccagggcg gaaattcttt
     7081 cgggcggtaa accctcgtgc accgtgccaa gaatcagctc tgcctctctc tttgttttga
     7141 gaatgcgaac tttttccggt cttttctttc tcaaattatc gccaaagctt cggaggcgtc
     7201 gaatctcttc ttcctcgctc gaaatacccg aaaatccttc caggagaatc aaatgcgaaa
     7261 tctcatccgg ataaagccct gcaaaacgag ctgccatcgc cgcacccatg ctgtggccca
     7321 ttagaacgta tggcttcttc acagtaccgg tgaatcgaat cagatctcca aaataagaat
     7381 gtgctccgta gtagctttcg cgaagacgag atgaatcgcc atgaccgcgc atgtccggga
     7441 acagcaattc aaatcggttt gcgagtgggg cctgtacaaa ttgaaaagtt tcccccgtat
     7501 ctgcgaaacc atgcagacct acgagttgtt gcgcgtccgg tcgcggaatc ttcagcacgg
     7561 acagatggcc gcctggcaat tcgaattgat agcgttccct agtttccaga ctttaacctg
     7621 cgaagagaaa gcatgatccc gagagccgcc agcgcatgat tgatttctcc cctggccact
     7681 ctttcttcca gctcgtcgag cgggacaaga acgatttcaa tgtcttccga tgaatcggga
     7741 aaatgctctc tattaggatt gatgaagcat ccttccgcga ggaagaaatg gattttgttt
     7801 gaaaaaaggg ccgggtttgg attgaaagag gcaatgtgcg tcattcgttc acttgtgtag
     7861 ccggtttctt cttccagttc tcgcgcggca gcaacaagcg gatctgtttc cgccgcatcc
     7921 acagcaccgc ccggagactc aagaattagt tccatcgcgc ccgcacgtgg ctgccggatg
     7981 agaagcgcct caccttcgcg cgtaatagga agtatgttcg cccagtcagg tgcacggagt
     8041 ctgtgaaaat cgtgtaacgg gccgcgcacc ctgtccacaa gagaaagcct ctgcacagta
     8101 aacgggaccg tgctgaggag atcttcctcg tttgtaagaa tccatttcat caaaactgat
     8161 agtgaaacgc aagattgaaa ccgtcttgcg aaggtgtcac accaacagaa gcacgaggag
     8221 caaagatcaa tacgtcaacg aggttgcctg catacaatcc aagcgccacg cctctggccc
     8281 aacggcctcg acgggcggcc tttgccatac cgttatacgc tttctgtgtc tgatccgttc
     8341 cgagaaagaa gaccgtggaa gcctggtagt catcctgcgc gtatccgagc agtcgagtca
     8401 cgaatggagt agaaaaaaga aatgtattgg tgatatcatg atactcaccc agtcgccgct
     8461 tgtatatctg atcctgatag ttcgagacag cagcagcgcc cagaaccccg cccatgtatg
     8521 cgaagccggc aatgcgtctc ccctggtaca actgccccca acctggcaac acgagagatc
     8581 taaaaaaggc tccggatcgg gttggctgtg attggcgttt cgcttcctct tccaggcgtc
     8641 tacgctcttc ttcgcgtcga cgttgttctt gtttttgaag ctccagctgc acttcctggc
     8701 ggactcgttc ccgaatctgc tcttccgtaa cgcgcaattc atcggttttc ttttcttccg
     8761 acttctttaa ctcttcctgc tgtttcaaca attccgcttg cttcttttct tctgctgctt
     8821 tgcgggcttc ttcttccttc ttcaggcgat ccgcgtctgc tgccttctgc tcttcagatt
     8881 tttttgaatc ttcaattttc ttaagttctt ctgctttctg cttttcttcc gcctggcgct
     8941 tttcgtctgc aagcttctgg tctgcgcgat tcttatcttc ttccgctttt ttcttttctt
     9001 ccgctagctt ctgatcttcc ttcttcttat cctcttccgc ctttttctgc tcttcgcgct
     9061 tcctggcgtc cctggtctgg tcttccgggg tcggaccata cgtaatcctg cgaatatcaa
     9121 tctttcgcaa caccaccctg cccgccgcag tatcaaggat cacttcgttt cgggtctggt
     9181 tcacaatgcg accattgtag acctgaccag ttttcataat gatctgctcc gcttggatcg
     9241 cagaaatcga aacaaaaaac gcaagaatcg taaacgcggc gaacttcatc aaaattttct
     9301 ctcctgttag agttatcggt tgtaaagcat agcgtcaatt caaaagcaag cgcatgtctt
     9361 acagcatcca ggacgcaaaa ccagggttgg tggctttcaa agattcgcct gttggaagaa
     9421 acatcctggg cggttgtctt ctggccgtgg ggcttctgtt at
//
LOCUS       DAORFF010000070         9460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3406_length_9460_cov_3.542052, whole genome shotgun sequence.
ACCESSION   DAORFF010000070 DAORFF010000000
VERSION     DAORFF010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9460
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3406_length_9460_cov_3.542052"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PKK76_04475"
     CDS             complement(<1..555)
                     /locus_tag="PKK76_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNL67752.1"
                     /translation="MEKAGGIAESLILERCLAPTSFLDSDHPAVQEYAHTHSGSGSAL
                     DRAVKLYYSVRDGWKYNPYKVSTDPIKSKASWVLTQTNGYCTTKALLLAALGRSIGIP
                     TRLGFGDVRNHLSSPRLIEYLKSEIFAWHGFTEFYLEGHWVRCTPAFDSALCRKFGVA
                     ALEFDGRTDSLFHPFDGEGRKFMEY"
     gene            complement(522..1886)
                     /locus_tag="PKK76_04480"
     CDS             complement(522..1886)
                     /locus_tag="PKK76_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNL67753.1"
                     /translation="MALQRQLGFITGALVVVASMVGSGVFYLPQIILGLTQNGWVFML
                     TFLVGGLIAITGSLSYAEMATMFPDDGGEYQYLKKTFGFLPAFLTGWISLLVGFTASI
                     TGSALVLKMYFVPLMDQMLGAENIFHNALFMKFFLACIIITLGTLHIIGVKFGSSVQN
                     ALTVIKMTIVISFILAGLYVLKGADFSQALSWVGPKAETSGLLIYSAALINVMYIYSG
                     WNGATYIGGEMKDPQKNLPRALLSAAVLTTVLYLLLNLVFIAAVTPEKMAGNPGFGGM
                     TAARLFSPGVSTLYGAFIVVILLSSISVQLMIGPRVYFKMAEDGVLFRFLSTVPGKSS
                     SPVYAIITQIALSIFYVLVFDDDELFSYMGFALSVFPILTVIGVMYLRRTQPNAVRPF
                     KVPGYPVVPAIFILLSSAMLALSLANAFTSEKKFGYIMALVVVLGGIPIYYVWKKLAA
                     SQKA"
     gene            1974..3449
                     /gene="mqo"
                     /locus_tag="PKK76_04485"
     CDS             1974..3449
                     /gene="mqo"
                     /locus_tag="PKK76_04485"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HNL67754.1"
                     /translation="MGKPEHSEVVLIGAGIMSATLGMMLKELDPGIRIHVFERLPEAA
                     RESSDAWNNAGTGHSAFCELNYTPQKADGSVDVSKAVKVASSFEMSREFWSHLIEKGR
                     IDSPKRFIHRIPHMSFVWGENVPFLRKRYAAMSASPLFSGMNYSEDWSLLSDWMPLVM
                     QSRAKENVAATRMDIGTDVDFGSLTRAMLNHLLMEGVDLHMEHEVRDIVRHENGQWNL
                     TICDLATQEEFKTRADFVFIGAGGGSLTLLGKSDIPEADGFAGFPVSGQWLKCTNQAV
                     VDMHQAKVYGKASVGSPPMSVPHLDTRMIGGKKALLFGPYAGFTTKFLKKGSYLDLAA
                     SLELENIFPMLSAGMHNLPLTKYLIQQALQTPEDRIEELRVYLPDAKIEDWELAVAGQ
                     RVQVIRADEEKGGVLEFGTEIVSAKDGSLSALLGASPGASTSVSIILEVLQECFPAQM
                     ASNGWKKKLKEMIPTYGENLAGNAALVKKSRARTASLLELD"
     gene            complement(3467..4312)
                     /locus_tag="PKK76_04490"
     CDS             complement(3467..4312)
                     /locus_tag="PKK76_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67755.1"
                     /translation="MRILIVGAGSVSGKSALQAAKAAGATVIATTSRKENLPGADHTI
                     HGIDLENPAAVDLILADRFLTQQPLDYIAYIPARGAVGISLEEAEASMVEPSLSYSVR
                     PYLKLHKALHPKKTIALSGFITMEPLLKIYGAMCFTKIAMEQLAVDHPKDLQIIRIGM
                     FHSNSVRGIMILAQRRFMRDPSYQPEWRAEWKASGKKFPEFFYAKNYKCEEETYRIHG
                     QGKPFRPTEPSDIAEGFRLAFSGESKPIINVLGPWLWTEDSMPVLPESIQSRMHFVPT
                     DLNLI"
     gene            complement(4359..4847)
                     /locus_tag="PKK76_04495"
     CDS             complement(4359..4847)
                     /locus_tag="PKK76_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67756.1"
                     /translation="MKSLSTPIQFVVAVLCAACVTSLQAQSGLRIEASYASIEHSMDS
                     NSTHETFTLAGSKLTYNYEYGGYHPGPNFERKRTDSKKVNPEDLHKFVMEKKLNRSLN
                     RKVVSSSPHAREVHLHLDIRMGAETFVLNFDGTRPDAGADKDFDNALAFCDLMRSIVS
                     NP"
     gene            complement(4864..6630)
                     /gene="cobT"
                     /locus_tag="PKK76_04500"
     CDS             complement(4864..6630)
                     /gene="cobT"
                     /locus_tag="PKK76_04500"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNL67757.1"
                     /translation="MGENRTPFSEEQIKGVYRAIHERRDIRAYRPEAVEDGVLQRLLE
                     AAHHAPSVGFMQPWNFILITDKARRRALYEHFLQCNEEASQHFEGERKLKYDSLKLQG
                     ILDAPINIVFTCDRSRGGKHVLGRNTIPDTDIYSTCLAIENFWLAARAEGLGVGWMSI
                     HKIETLHSILEIPAHVLPVAYLTLGYPVSFPDAPLLESTGWRAREDLTNLVYENVWGQ
                     LKEFAPKEQPPKAGGHIQTSTESSAGRSIGREAETVRHRLDHLTKPRGSLGDLEDWIV
                     HLAAIQKRNVPLCDHPHILLFAGDHGIAAHHISAYGQHVTAQMMYQYVAGTAAINSIA
                     RANGIALSIYDVGVNHDFGNAEGIQHRKVKMGTRDFQIDCAMTPDECRAAYEHGRSSV
                     LELPDTCSIIGLGEMGIGNTTAASAIACALLGDSAENLTGAGTGIGPDTRKRKIEIIE
                     KGLSLHRQQIASPMDAIARLGGYEIASLAGAIEGAFEKNIPVVLDGFITGVAALAAVR
                     HRPEVHSVLIAGHSSKEKAHAQILAKLNLRPILNLNLRLGEGSGAALAMGLIANACRL
                     YSDMKTFEEANIIFAENEQARL"
     gene            6694..7530
                     /gene="cobA"
                     /locus_tag="PKK76_04505"
     CDS             6694..7530
                     /gene="cobA"
                     /locus_tag="PKK76_04505"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNL67758.1"
                     /translation="MSAWCDPLEKFTLVGLEEGQHPKGKVYLVGAGPGDPDLLTIKAS
                     KIIQSADVILYDSLIGPEILKFASPNARRIFVGKRAHSHSYTQDQINEMILEEASKHH
                     TVVRLKGGDPFIFGRGGEEIQFLREHGIELEVIPGVTAATAASALLQSPLTHRDLGAS
                     VVFLSGYSRADRESDDGFPHHDWKYLASSSLTLVFYMGQHHLSRICQSLIEAGREENT
                     SIALVSNISLPEESVTLTTISHARDPEVSRSVRFPAVAIVGDVLRMMDPALKARIAKS
                     HS"
     gene            complement(7499..8107)
                     /gene="thrH"
                     /locus_tag="PKK76_04510"
     CDS             complement(7499..8107)
                     /gene="thrH"
                     /locus_tag="PKK76_04510"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF010109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HNL67759.1"
                     /translation="MRPTIFVMDLEGCLIPEIWLHIADKTGIADLRKTTRDVADYREL
                     MNGRLAVLDRHKIKLSDIQAMLADLGILEGARDFVEWVREKTEFIILSDTYVEFAKPL
                     MKGLGNPTLFCHRLTTNAAGQIDGYALRSEDSKADIVRALKGNGFRIMAAGDSFNDSN
                     MLIQADNARWFRAPASIVEKFPQFPALTEYQDLKNAISQFLL"
     gene            8258..9004
                     /locus_tag="PKK76_04515"
     CDS             8258..9004
                     /locus_tag="PKK76_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67760.1"
                     /translation="MKFASAICILFFASLSGLIAKPGDYKELGGGWDGTRAMTALDGA
                     IYAVHGGKLYRTDSSGKYTELGDGGWSETSAMCGMDGALWIIDGGKLYRVDKNGKYAE
                     VASGWSKTRLMTTLDGSLWIIDNGTLYKVDKNGKYVEVGEGGWSKSSAMVAFDGALWI
                     IDSGKLYRTDKTGKYTELGDGGWSSTSAFVAAGGSLWAVDGNKLYKIDRAGKYTEIGG
                     GGWTEVEGAASIGDDLFIISGSKLYRASTK"
     gene            complement(9010..>9460)
                     /locus_tag="PKK76_04520"
     CDS             complement(9010..>9460)
                     /locus_tag="PKK76_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67761.1"
                     /translation="NPADLEAIRSAILAEYKNRIYPNEINLYPSRTDRSKNSSAMQGF
                     AENDAYTFIATGERAPGNAPADSQRRLILRQMKEENKSIWDSDYEYFNGISRTYSYNH
                     EQESYWITEIDIIAASFCNERALTEEEMLAALSDFSLHHLRESGFTF"
BASE COUNT         2232 a         2272 c         2508 g         2448 t
ORIGIN      
        1 atattccata aactttctgc cttcgccgtc aaaaggatga aagagactgt ctgttcttcc
       61 atcgaattca agagctgcta caccaaactt gcggcacagt gccgaatcaa acgctggagt
      121 gcagcgaacc cagtggcctt ccaggtagaa ttcagtaaag ccatgccagg caaagatttc
      181 gctctttaga tattcaatca gcctgggact cgaaaggtga tttcgcacgt ctccaaaacc
      241 aagccgtgta ggaattccaa tcgaacggcc aagagctgcg agcagaaggg cttttgtggt
      301 gcaatatccg ttcgtctgag tcaacaccca gcttgctttc gatttgatag gatctgtgct
      361 tactttgtac ggattgtatt tccagccatc gcgcacactg tagtaaagct tcactgcgcg
      421 atcgagcgcc gatccacttc cagaatgagt gtgcgcatat tcctgaactg cgggatgatc
      481 cgagtccagg aatgaagtcg gcgcaaggca gcgctcgaga atcaggcttt ctgcgatgcc
      541 gccagctttt tccatacgta gtaaatagga atgccaccga gaaccacaac gagtgccatg
      601 atgtatccga atttcttttc gctggtaaac gcgtttgcaa gggaaagtgc gagcattgcg
      661 gaagaaagca aaatgaaaat ggccggaacc acggggtatc ctggaacttt gaacggtctt
      721 acagcgtttg gctgggtgcg gcgcagatac attacgccga ttacagtgag aatgggaaat
      781 acgctgagag cgaagcccat ataagaaaag agttcatcat cgtcgaagac caacacgtag
      841 aaaatgctca gagcgatctg agttataatc gcgtaaaccg gagaagatga tttgcccgga
      901 acagtggaaa ggaatcgaaa cagtacaccg tcttccgcca ttttgaaata aacgcgagga
      961 ccaatcatca gctgaacgga tatggaagac aaaagaatca caacaatgaa cgcaccgtag
     1021 agtgtagaaa ctccagggct gaatagacgc gcggccgtca tgccaccaaa tccagggttg
     1081 cctgccatct tctccggagt taccgccgcg atgaagacca gattcaagag caaatagaga
     1141 acggttgtca gaactgcagc agacaagagc gctctgggca ggtttttctg tggatctttc
     1201 atttccccgc caatgtaggt ggcgccattc cagccagaat agatgtacat cacgttgatc
     1261 agtgctgcag aataaatcag aagccctgat gtttccgcct ttggtcccac ccaggacaga
     1321 gcctgagaga agtctgcgcc tttaagaaca taaagtcccg cgagaatgaa tgagatcacg
     1381 atggtcatct tgattactgt gagagcattc tgcacgctgc ttccgaattt cactccgatg
     1441 atgtgcagag tccccagtgt gatgatgatg cacgccagaa agaatttcat gaacagcgca
     1501 ttgtgaaaaa tattctctgc acccagcatc tggtccatca atggaacaaa atacatcttg
     1561 agaacgagtg cgctaccggt gatcgaagcc gtgaatccca ccagaagtga aatccaaccg
     1621 gtcaagaacg ctggtaaaaa tccgaatgtt ttcttgagat actggtactc cccgccgtcg
     1681 tctggaaaca tggtggccat ttccgcgtag gaaaggctgc ccgtaattgc aatgagaccg
     1741 ccaaccagaa atgtcagcat gaaaacccag ccgttctgag tcagtccgag gatgatttgg
     1801 ggaagataga atacgccaga tccaaccata cttgcgacta cgacgagtgc tccggttatg
     1861 aatcccagct gtctttgaag tgccattcca tccgatatgg cggtgcactg catcgccgca
     1921 agttcttttc ggaaagggat tgacgacgct acattccgtc gctttcaaca ggtatgggca
     1981 aaccggaaca ttctgaagta gtattgatcg gtgcgggaat catgagtgct actctcggga
     2041 tgatgctcaa agaactcgat cccggaattc gaattcacgt atttgagaga cttccggagg
     2101 ctgcgcgcga aagttcagat gcctggaaca acgcaggcac ggggcattct gccttttgtg
     2161 agctgaacta cactccccag aaagcggatg gcagtgtaga tgtgagcaag gcagtgaagg
     2221 ttgcgtcttc tttcgaaatg tcgcgcgaat tctggtctca cctgatcgag aaaggacgga
     2281 tagattcgcc caaacgtttt attcatcgca ttccacatat gagttttgtc tggggagaaa
     2341 atgttccatt tttgcggaaa cgttatgcgg ccatgtccgc ctctcctctg ttttcaggaa
     2401 tgaattattc tgaggactgg tctctccttt cggactggat gccgcttgtt atgcagtctc
     2461 gcgcaaaaga gaacgttgcc gccacgagga tggacattgg tacagatgtt gattttggct
     2521 ctcttaccag agcgatgctg aatcatcttc tgatggaagg agtagatctg catatggagc
     2581 acgaagtgcg cgacattgtt cgtcatgaga acgggcaatg gaatctaacg atatgcgatc
     2641 ttgcgacgca ggaagaattc aaaacaagag cagattttgt attcattggt gcaggcggag
     2701 gatcgctcac gttgctcggg aagtcagata ttccggaggc ggacggcttt gctggatttc
     2761 cagtcagcgg acaatggctg aagtgcacaa atcaggccgt tgtggatatg caccaggcaa
     2821 aagtgtatgg taaggcgtct gttggatctc cgcccatgtc tgttcctcat ctggacactc
     2881 ggatgattgg cggaaaaaaa gcgcttctct tcggaccgta tgcgggattt accaccaagt
     2941 ttttgaaaaa gggatcctat ctggatctgg ccgcgtctct ggaactggag aatattttcc
     3001 ccatgctttc tgcggggatg cacaatcttc cgctgacgaa gtatctgatc cagcaagctc
     3061 tgcagacgcc agaagataga atcgaggaac ttcgcgtcta tctaccggat gcgaagatag
     3121 aagactggga gcttgctgtg gcaggccaga gagtgcaggt gattcgggcg gatgaggaaa
     3181 agggtggagt gctcgagttt ggcacggaga tcgtatctgc gaaggacggt agcctttccg
     3241 ctttgctcgg agcttcccct ggcgcgtcta catctgtttc tatcattctg gaagtactcc
     3301 aagaatgttt tcctgcacaa atggcgtcta acggttggaa gaaaaagctc aaagaaatga
     3361 ttccgactta tggcgaaaac ctggcgggca atgccgcact tgtgaaaaag agccgcgctc
     3421 gcactgcttc tcttcttgaa ctggactgat tttgtaagac tgcgactcag atcagattca
     3481 aatctgtcgg aacaaagtgc atcctgctct gtatgctttc tggcaggact ggcatgctgt
     3541 cttctgtcca gagccacggc ccaagcacgt tgatgattgg tttagattct ccactgaatg
     3601 ccaggcgaaa gccctctgcg atatcagaag gttccgtggg gcgaaatggt tttccctgtc
     3661 cgtggattct gtacgtttct tcttcgcatt tgtagttctt cgcgtagaag aactccggga
     3721 atttctttcc ggatgctttc cattctgcgc gccattcagg ctggtaagaa ggatctcgca
     3781 taaatcgtct ctgtgcgaga atcatgatgc ctctgactga attgctgtga aacattccaa
     3841 ttcgaatgat ctgaagatct tttggatgat ccactgcaag ttgttccatg gcaattttgg
     3901 tgaagcacat ggctccgtag atcttcagca aaggttccat cgtaatgaaa ccggaaagcg
     3961 caattgtctt tttcggatga agggccttgt gcagtttgag atagggacgc acagaatacg
     4021 aaaggcttgg ttccaccata gacgcttccg cttcttccag agaaattcca actgcacctc
     4081 tggccgggat gtaggcaatg taatcgagtg gttgttgggt taagaatcga tctgcgagaa
     4141 tcagatctac ggccgctgga ttttccaggt ctatcccatg aatcgtgtga tcggcgcccg
     4201 gaaggttttc tttcctgctg gtggtggcta tgactgtggc cccggccgct tttgctgcct
     4261 gcaatgccga tttgcctgat acacttccgg cgcccacgat tagaattcgc atgggtgacg
     4321 cttccttgca cgtcttcgcg cgcaatagtt tttcgatact aaggattgga tacgatggat
     4381 cgcattagat cgcagaatgc aagagcgttg tcgaagtctt tgtctgcccc cgcatccggt
     4441 cttgttccgt cgaagttcaa aacgaatgtc tctgcaccca tgcgaatgtc aaggtgcagg
     4501 tgaacctccc tggcatgcgg gctcgaagaa acaacctttc ggttgagaga tcgattgagc
     4561 ttcttttcca taacaaactt gtgcagatct tcaggattta ctttcttgct gtccgttctc
     4621 tttctctcaa agttcgggcc ggggtgatag ccgccgtatt cgtagttgta ggtgagtttg
     4681 ctgcctgcca gagtgaacgt ctcatgagtt gaatttgaat ccatcgagtg ttctatgctc
     4741 gcatagcttg cctcaattct gagcccggat tgagcctgga gagatgtcac gcacgcggcg
     4801 caaagcactg cgacgacgaa ctggatgggg gtgctcaatg atttcatgta tttctctgac
     4861 gctctaaagt cgcgcctgtt cattttcagc aaagataata ttcgcttcct cgaaggtttt
     4921 catgtccgaa tatagccggc atgcgtttgc gatgagtccc atggccaggg ctgctcctga
     4981 gccttcgccg agccgcaggt ttagattcaa gatgggtcgc aggttcagtt ttgcgagaat
     5041 ttgcgcgtgt gccttttcct ttgaagaatg tccggctatc aaaacagaat gcacttcagg
     5101 tctgtgtcgc acggcggcga gtgctgccac acctgtgatg aatccgtcca ggaccacggg
     5161 tatgttcttt tcgaaggcgc cctctatggc tccggccagg cttgcgattt cgtaacctcc
     5221 gaggcgggca atcgcatcca tcggggaggc aatttgctgc ctatgaagtg agagtccttt
     5281 ttctatgatt tcgattttcc tctttctggt gtctggtccg attccagtgc cagcgcccgt
     5341 gaggttttcc gcagaatcac ctaacaatgc gcaggcgatg gcagatgctg cggtagtatt
     5401 tccgattccc atttcaccca ggccaatgat agagcatgtg tccggtagtt ccagaaccga
     5461 gctgcgtccg tgttcgtagg ccgctctaca ttcatccggg gtcatagcac aatctatttg
     5521 aaagtcgcgc gttcccattt tcaccttacg atgttggatt ccctccgcat ttccaaaatc
     5581 gtggttcact ccgacgtcat atatggacag agcaattcca tttgcacgcg cgatagaatt
     5641 gatagcagct gtgccggcta catactgata catcatctgt gcggtcacgt gctgcccgta
     5701 ggcgctgata tggtgtgctg caatcccatg atctcccgca aaaagcagta tgtggggatg
     5761 atcgcataac ggcacatttc tcttttgaat cgcggcaagg tgcacaatcc aatcttcgag
     5821 atcccccaga gagcctctgg gcttggtcaa atggtccagc cgatggcgca ctgtttctgc
     5881 ctcgcgcccg atgcttctac cggcactgct ttccgtgctt gtctgaatgt ggcctccggc
     5941 cttcggaggc tgctctttgg gcgcgaactc tttgagctgg ccccagacat tttcgtacac
     6001 caggtttgtc aggtcttcgc gcgccctcca cccggtcgac tcgagcaaag gtgcatctgg
     6061 aaaactgact ggatagccca gggtgagata ggccacaggc agaacatgcg ctggaatttc
     6121 tagaatggaa tggagcgttt cgattttgtg gatgctcatc cagcccacgc ccaaaccttc
     6181 cgctctcgca gccagccaga agttttcaat ggcaaggcat gtgctgtata tatccgtatc
     6241 cgggatcgtg tttcttccca gtacgtgttt tcctccgcga gatcgatcgc aggtgaatac
     6301 tatgttgatc ggtgcatcca gaatcccctg aagtttgagg gaatcgtatt tgagttttcg
     6361 ttctccttca aagtgctgag atgcttcttc attgcactgt agaaaatgtt catagagggc
     6421 gcgtctcctc gctttgtctg ttatgagtat gaagttccac ggctgcatga agccaaccga
     6481 tggcgcatga tgagccgctt ctagaaggcg ttggagtact ccgtcttcga ctgcctcagg
     6541 gcgatacgcg cgtatatcgc ggcgctcgtg gatggcgcga tagactcctt tgatttgctc
     6601 ttccgaaaac ggagtgcggt tctcacccat ccttgcatcc gttcttgttt gggccgcctg
     6661 tcaagtcaga gtgaatcagg ttgattctta aaagtgagtg cgtggtgtga tcctctggaa
     6721 aagtttacac tcgtgggcct ggaagaagga caacatccaa agggaaaggt ttatctcgtg
     6781 ggcgccggtc ccggtgatcc agatcttctt acgatcaagg caagcaagat cattcaatct
     6841 gcagatgtga ttttatacga cagtctcatc gggcccgaga ttctcaagtt cgcttccccc
     6901 aatgcgcgaa gaatatttgt aggtaagaga gcgcatagcc atagttacac gcaggaccag
     6961 atcaatgaga tgattctgga agaagcatcg aagcatcaca cggtggttag gctcaagggt
     7021 ggagatccgt ttatttttgg gcgaggtggt gaggagattc aattcttgcg tgagcacgga
     7081 atcgagcttg aggtgattcc cggcgtcacc gctgccaccg cggcctccgc gctcttacag
     7141 agccctctca cacatcgcga tctgggggcg tctgttgttt tcttatccgg atacagtcgt
     7201 gcggatagag aatcggacga tggctttcca catcatgact ggaagtacct ggccagttcc
     7261 tctttgacac ttgtcttcta tatgggtcag catcatcttt cccggatctg tcagtcattg
     7321 atagaggccg gaagagaaga aaatacttcc atcgcacttg tgtccaatat cagcttaccg
     7381 gaagagtcag tcactctcac tacaatctcg cacgccaggg atcctgaggt atccaggtct
     7441 gttcgttttc ctgctgtggc gattgttggg gatgttctgc ggatgatgga tcctgcactc
     7501 aaagcaagaa ttgcgaaatc gcattcttaa gatcctggta ttcagtgagc gcggggaact
     7561 gcggaaattt ctctacaatg gatgcaggcg cgcgaaacca tctggcgtta tccgcctgga
     7621 tcagcatgtt ggagtcgttg aaagaatcgc cagcggccat aatgcgaaat ccatttcctt
     7681 taagtgcccg cacaatgtcc gctttgctgt cttcgctgcg aagagcatag ccgtctatct
     7741 gtcctgctgc gtttgtagtg aggcgatggc aaaatagagt ggggttccca aggcccttca
     7801 tgagaggctt tgcgaattcc acatacgtat ctgaaaggat aatgaattct gttttttcgc
     7861 gcacccattc tacaaagtca cgcgcgcctt ctaaaattcc aagatcggcg agcatggcct
     7921 gaatgtctga gagtttgatt ttgtgccgat ctagaaccgc aagcctgccg ttcatgagct
     7981 cccggtagtc ggccacgtcc ctggttgtct ttctgagatc tgcgattcca gtcttgtctg
     8041 cgatgtgtag ccagatttcc gggatcaagc atccttccag gtccattaca aatatggtgg
     8101 gacgcatttg agtccttttc gccggaagtg gcctcccgga caattgcttt tttaaaagag
     8161 atgtctctgc ctccggcttt ctccgagaga tacgggattt attccaggtg atcaaaaccc
     8221 gtttgactcc ttcttgctcc tggtctggac tagagaaatg aaattcgcat ccgccatttg
     8281 cattctattc ttcgcatctc tctcaggtct gatcgcaaag cccggggact acaaagagct
     8341 tggcggtgga tgggatggta cgcgtgcgat gactgcactc gacggcgcca tttatgccgt
     8401 tcacggaggc aaactttaca gaacagactc gagcggaaaa tacactgaac tgggtgacgg
     8461 aggctggagt gaaacttctg cgatgtgcgg gatggacgga gctctgtgga tcatagacgg
     8521 cgggaaactc tatcgcgtgg ataagaatgg aaagtatgcg gaggttgcca gtggctggag
     8581 caagaccagg cttatgacta cgctcgatgg ctcactctgg atcattgaca acggcactct
     8641 ctacaaagta gacaagaatg gaaaatatgt agaagtgggg gaaggcggat ggagtaagag
     8701 cagtgcgatg gttgcttttg acggagcact ctggatcata gacagcggca agctctacag
     8761 aacagacaag actggaaaat acactgagct cggagatgga ggatggagtt ctacttctgc
     8821 ctttgttgct gcgggtggtt ctttgtgggc tgtggacggc aacaagttgt ataagattga
     8881 ccgcgcagga aagtacacag agatcggcgg aggcggttgg actgaagtgg aaggcgcagc
     8941 atcgatcgga gatgatctgt tcattatttc cgggagcaaa ctctatcgtg cttccacgaa
     9001 atagaggcct cagaaagtaa aaccactttc gcgtaaatga tggagcgaaa aatcgctcag
     9061 agcggccagc atttcctctt cggtgagcgc gcgctcgttg cagaagcttg ctgctatgat
     9121 gtctatttcg gtgatccagt agctttcctg ttcgtgattg tacgaatagg tccttgagat
     9181 tccgttgaag tactcataat cagaatccca gatgcttttg ttttcttcct tcatttgcct
     9241 gagtatcagg cgccgctgcg aatccgctgg agcatttccc ggagcgcgct ctccggttgc
     9301 gatgaaagta tacgcgtcgt tttcagcgaa tccctgcatt gcagaagaat tcttgcttct
     9361 gtcggtcctt gacgggtaca gattgatttc atttggatag attctgtttt tgtactctgc
     9421 cagaatcgcg ctccggatgg cctccaggtc ggcgggattg
//
LOCUS       DAORFF010000071         9458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3409_length_9458_cov_3.358396, whole genome shotgun sequence.
ACCESSION   DAORFF010000071 DAORFF010000000
VERSION     DAORFF010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9458
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3409_length_9458_cov_3.358396"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PKK76_04525"
     CDS             <1..589
                     /locus_tag="PKK76_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNL67762.1"
                     /translation="EIIQSDVGDANQRIRLIEDVTARFPDLNVLVNNAGIQRRLDLTR
                     PEPFDATRSEISINLEGPVHLSMLVLPHLMKKKKAAIVQVTSGLAFTPLAFVPVYSAT
                     KAAMRSFTLSLRHQLRGSPVEVIEIIPPAVQTDLGGPGHHDFGAPLQEFADDVMRRLD
                     QGETEIAYGFAMQASKASRQDLDAIFQRMNSPAGS"
     gene            591..1883
                     /locus_tag="PKK76_04530"
     CDS             591..1883
                     /locus_tag="PKK76_04530"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL67763.1"
                     /translation="MTARNRLAGLLARQIHHHLREELQKPSSAVEFITVEGVPFKDLT
                     AKMIADLEKEFPELLTPRELPFSLMQAWWDESNPAVPYAQSTFKTYNQLMLFRVLDLK
                     HNSEFLRKKFEEKMIANGHALIGRGRNLGFETIFPAETRMLQMISRLARIEGIPDEET
                     IPLLLDQLILTTQTAYRMFDSLFRESLNQTDKILKSVLPEKIADELKKTGKVAPVHVD
                     SASVLFSDIVGFTRIAEELPPAQLLAELDRTFAHFEAAMKPNHLEKIKTIGDCFMCVG
                     GISERNRLHEVDTVLAALRIQEFMRKYQKSRARTGSPSWKLRIGIHSGPVVAGVLGQE
                     RFNYDIWGDTVNVANRMESAGEPDTINISEVTAQAVQEFFILEPRGRIFVKNKGEMEM
                     YFVRGILPELSVNGGSRVPSHEFARRYATKRRAIRAKS"
     gene            1930..3057
                     /locus_tag="PKK76_04535"
     CDS             1930..3057
                     /locus_tag="PKK76_04535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNL67764.1"
                     /translation="MPRWLAAVPEKVRREITNNNLSRAWIAGLVMLPVHFAHVLLFYF
                     RVVQPEEESWRWGIIQSHLLAAAFWIPALPVLRFFMNRRERWIGPAHFLIFAAVIGYV
                     FLGAIVCAVDQQVAPAPMAYFVAVIGVAILLRLPPVETLVIFAAGLIFVWMAVGITQT
                     NPTYRLFVRVNCITASGVGLIVGASLWYYEVRTVEQRLQIEEQNSALETLAVRDPLTG
                     LFNRRRFLEALDLEIARIRRTGQGGAILWLDLDDFKAINDKFGHPAGDAVLLHVAAIL
                     TGCVRVTDTVARFGGEEFIVLLPDTDQKGALETASRILNTLRETTVTFRGAELSVTAS
                     CGAAAIEIIPPDPAGATIQKADTALYRAKKTGKNRVEHSFS"
     gene            3054..4091
                     /locus_tag="PKK76_04540"
     CDS             3054..4091
                     /locus_tag="PKK76_04540"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL67765.1"
                     /translation="MKPKKKKENGLRTATYASLASAVISAVASYFVPAYGRAPLDSAL
                     VGALIGFLIPFSLSGIAALAQGGASFKKLPYAGYLVTISLLIAADTLVIYFIIGLLLF
                     PANVLDQSQLILAGGLSAGISVTFSVANVLRQFIGGSVLADIFLGRYHRAKEEIRSFV
                     FIDLVSSTALAESLGSSLFFRMINDFHSVVERATRANKGILYKYMGDGQILVWPGTLE
                     GRTSAILACLEIQRELAELRERFESKYNHAADFTAGLHCGPVLIGEIGDERKEIGYWG
                     DTVNTTQRIQSSCKTYHCSILASEAFMDQIQSLPARLSVAQNVPLRGKSNPVNLIRIE
                     SLTASETDSHS"
     gene            4165..4473
                     /locus_tag="PKK76_04545"
     CDS             4165..4473
                     /locus_tag="PKK76_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67766.1"
                     /translation="MTGPFRHNIQWRVRARAHLRGQELAVPLEGIDECFLTRWIDSRP
                     DLANRPQCVSLKRAHEALHKIVAKKDSGPATTDLLQDFEIQSRAVMTAIETMRQAIRP
                     "
     gene            4505..4870
                     /locus_tag="PKK76_04550"
     CDS             4505..4870
                     /locus_tag="PKK76_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL67767.1"
                     /translation="MKLLIVDDSNIIRKAIQKYLAEFGLEIVGEAGDGRTALTLFRET
                     SPDVVTMDITMPEMDGLETLSEMRKISDSPKIIIVTALKDKATALDALQKGASGFLAK
                     PFTEAQLKEEIGRAIGRAS"
     gene            4867..5316
                     /locus_tag="PKK76_04555"
     CDS             4867..5316
                     /locus_tag="PKK76_04555"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNL67768.1"
                     /translation="MTQKDLQIFVNGTVNYFKQLTDSGAQMDVPFLKKPGETAALDIT
                     GVIGISGVKKGIIYFTASSDMLRDVVKTILGTENPDLASIVDIAGEIANTIAGNAREA
                     FGADFMISIPLVVEGPPKNIRIPNEVPVFVIPIRWKDHRSFLVVGIQ"
     gene            5313..6101
                     /locus_tag="PKK76_04560"
     CDS             5313..6101
                     /locus_tag="PKK76_04560"
                     /inference="COORDINATES: protein motif:HMM:NF039346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL67769.1"
                     /translation="MSAGAAFPVVDTDHAVEIAPEVFWTGYYMEDEALHCNPYLIRNG
                     TESILIDPGSVPDFPVVGRKLFSVIKPESVTTIILNHQDPDLCASVPIFESLRENFDY
                     DVIAHRKTIYLLKHYGIKGKFHRLDDGETAYKTPGGRELKFITTPFAHFAGAVMTYDV
                     KSKVLFTGDILGGVGTDWELYHTDIALANMKAFMQGFMPCNKALRYALLKIESSGAEL
                     FAPQHGQIIHRDQFPSIKEALWDLKCGLDLIEDDAMKKAMVHAV"
     gene            6098..8002
                     /locus_tag="PKK76_04565"
     CDS             6098..8002
                     /locus_tag="PKK76_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNL67770.1"
                     /translation="MTSREEITRAENYYRAISELTVARHREHLIKELLNYMASLLNAV
                     EEANSRLTFSEARIRAIVNTAADGILTYDESGNIDSINPAAAAMFGLNPESIQGLHVS
                     SLLEGESEGHLRTVNARQVGGGYEDVAKRGNNTFPVYIAVQDMRLADRMFHVAILRDI
                     TDQKNAQRLEAQARAKEETDKILSAIDQGLFLIYRDGARFEIGSQFSRATRDILGVDL
                     ESQKDLLSILGNRVAESTIAAAKNFLELMFKDSVREESLRSLNPLSEIPFASQTAGDT
                     RTLQFTFRRVTTDQKITHLMATVSDVTEEAALREQLKQNEQQSRTQMEMLLKILHVDS
                     DMLDEFIDETRLEIDIIDTILKKEDGDLRKRLETIFRTAHKIKGNADLLGLSFFAEQI
                     HSFEDTLSVLLRNEEISPADFLPVLFAFNKVTEIVDAIDALVERLLDFKSRFGQSNSG
                     DELILRSARALINKLTDETGKLAELHVRVFEARTLGTPKRKLIRDVITQLIKNSMMHG
                     IETPETRTAGGKRNPARIELNLRSGKDGIFLSYKDDGQGLHLSRLKEKAIKQGIATEA
                     EIATWPPAKTAELIFATGLSTSLTTGIQSGRGIGMGLIREAVHDVQGKIRILSAPAKF
                     FEVQIWIPVA"
     gene            7999..>9458
                     /locus_tag="PKK76_04570"
     CDS             7999..>9458
                     /locus_tag="PKK76_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67771.1"
                     /translation="MMSFQGAASILNLTATGMLLLYVAVKFIRSRSYAAALPWLLIRL
                     ATAAVTATTGLMIHWPPILITIIFLLLILEGLAAAFTATRVTVTRPHGRRLAVSIGIV
                     CIIASIAMIAFPDSGYTAFPFIVTAAWSAAFVCLVQGGIRKEIHGSLFFAQGLSLAGA
                     LLHLYLLRCSQEAILLIAASALQCLSLLFIFQRVADADLVTSSLKSRVLFSGTIVSLM
                     VALAGLGAADAFYQKLHAVDREHQLVEMSNELQENDFSHMPESIEYIVDSENSEIVFA
                     RNVRIQKIPFRETRSDIVKCETCKPETLCNLIQWSSPRHLRIFGPDLSFDIRTVNINS
                     HTYEAGIDVLDYRKYESERSTRTLLFSYAALVIVTMALFFFLRHTVLTPLSILMENLQ
                     SLAKGEPTRPMPRDELGAVNKVMTRLMHRLAKNKDALTRANAQLQKEKNILEKFFSND
                     FVEQLLQEKISHSLGGARVPATIVFLDIRNSTAIAE"
BASE COUNT         2517 a         2410 c         2347 g         2184 t
ORIGIN      
        1 ggaaatcatc cagtcagacg tgggagatgc aaatcaaagg atccgactga tagaggatgt
       61 cacagccaga ttcccggatc tcaatgtcct cgtaaacaat gcgggcatac agagaagact
      121 cgatctaaca agaccagaac catttgatgc aaccagaagc gagatcagca tcaacctgga
      181 gggacctgtc catttgtcca tgctcgtcct tccccatctc atgaagaaaa agaaagcggc
      241 cattgtacag gtgacgagcg gacttgcgtt cactcctctt gcatttgttc cagtctacag
      301 cgccaccaag gctgcgatgc gatcttttac tctttctttg cgccatcaac ttcgcggatc
      361 gccggtggaa gtcattgaaa tcattcctcc tgcagtgcag acagatctcg gaggaccagg
      421 acatcatgat tttggcgcac cactccagga attcgcagat gacgttatgc gacgactgga
      481 ccagggagag actgaaattg catatggttt tgccatgcag gcgagcaagg catccaggca
      541 agatctagac gcaatcttcc aaagaatgaa ctcgccggcc ggctcttgaa atgactgcac
      601 gcaacagact cgcgggtcta cttgccagac agatccacca tcacctgcgc gaagagctac
      661 aaaagccctc ctccgcagtt gaattcatta ctgtagaagg cgttccgttc aaggatttga
      721 ctgcgaagat gatcgcagac ctggaaaagg aatttccgga actgctcaca cctcgtgaac
      781 tcccgttttc cctcatgcag gcctggtggg acgaatccaa tccagccgtg ccttatgcgc
      841 aatccacatt caaaacatac aaccagctga tgctatttcg cgtcctggat ctcaaacaca
      901 attctgaatt tctgcgaaag aagtttgagg aaaagatgat cgcgaatgga cacgcactca
      961 taggccgcgg aagaaatcta gggtttgaaa ctatctttcc tgcagaaacg cgtatgctgc
     1021 aaatgatttc acgccttgcg cgcatcgaag gaattccgga cgaagaaacc atccctctcc
     1081 ttctagatca gctcattctc actactcaaa ctgcatacag gatgtttgat tccctgtttc
     1141 gtgagtcact caaccagacg gataaaatac tcaagtccgt tcttccagaa aagattgcag
     1201 atgaactcaa aaaaacggga aaagttgcac cggttcatgt agattcagcg agcgtgctgt
     1261 tttcagacat tgttggattc accagaattg cagaggaact tcctccagca cagctcctgg
     1321 cagaactcga tcgcacattt gctcatttcg aagccgccat gaaacccaac catcttgaga
     1381 aaatcaaaac gattggcgac tgcttcatgt gcgtgggcgg gatttcagaa agaaacagat
     1441 tgcacgaagt ggatacagtg ctcgccgctc ttcgcatcca ggaattcatg cgaaagtacc
     1501 aaaaaagtag agcgagaaca ggatctccct cctggaaact gagaattggc atccatagcg
     1561 gaccggtagt tgcaggagtt cttggacaag aacgattcaa ttacgatatc tggggagata
     1621 cagtaaacgt tgcaaacaga atggaatctg cgggggaacc agataccatt aacatttcag
     1681 aagtgaccgc gcaggccgtg caagagttct ttattctcga accgcgtgga agaatcttcg
     1741 ttaagaacaa aggagaaatg gaaatgtatt ttgtgcgcgg gattctgccg gaattgtctg
     1801 tcaacggtgg ttccagagtc ccttcgcacg aatttgcccg acgttatgcg accaaacgac
     1861 gcgctatccg tgcgaaaagc tgaactgttt tttgattgat caggttcgca accggcattt
     1921 caatgcgaaa tgcctcgctg gctggctgcc gtcccggaaa aagtacggcg cgaaatcaca
     1981 aacaacaacc tgagtcgggc ctggattgcg ggtctggtca tgctgccggt gcattttgct
     2041 catgttctgc ttttttactt tcgcgtagtc caaccggagg aagaatcctg gagatggggt
     2101 atcatccagt cacatctcct ggcggctgca ttctggattc cggcattacc tgtgcttcgc
     2161 tttttcatga acaggcgcga aagatggatt gggcccgcgc actttctaat atttgcggct
     2221 gtcataggtt atgtttttct gggcgccatt gtttgtgctg tggatcagca ggtagcgcca
     2281 gcgcccatgg cgtactttgt agcagtcata ggagtggcca ttctactccg cctgcctcca
     2341 gtggagactc ttgtgatttt cgccgccggt ctcatcttcg tatggatggc tgtgggcatc
     2401 actcaaacca atcccactta cagattgttc gtcagagtca attgcatcac tgcctctgga
     2461 gtggggttga tcgtgggcgc gtctctatgg tactatgaag tgcgcacagt ggaacagcgg
     2521 cttcaaatcg aagaacaaaa cagcgctctg gaaactcttg cagtgcgaga tccactcaca
     2581 gggcttttca atcgcagaag gttcctcgaa gcgctcgatc ttgaaatcgc gcgaatccga
     2641 agaacaggcc agggcggcgc cattctgtgg ctcgatctag acgacttcaa agcgatcaat
     2701 gataagttcg ggcatccggc gggagatgca gtgttgttgc acgtcgcggc cattctgact
     2761 ggatgcgttc gcgtgacaga tactgtggcg agattcggtg gagaagaatt cattgtactg
     2821 ttgcctgaca cggaccaaaa gggagccctc gaaacagcca gtcgaatcct gaacaccttg
     2881 cgagagacga ccgttacgtt tcgcggggct gagctttccg taactgccag ctgtggagcc
     2941 gcagccattg aaatcattcc tccggatccg gcaggagcca cgatccagaa agcagacaca
     3001 gcactctaca gagcaaagaa gaccggaaaa aacagagtgg agcattcgtt ttcgtgaagc
     3061 cgaagaagaa aaaggaaaac gggcttagga cggccaccta cgcttctctc gccagtgcgg
     3121 tgatcagcgc tgtggccagc tactttgttc ctgcttacgg ccgcgcaccg ctcgactctg
     3181 cacttgtggg cgcgttgatt ggatttctaa ttccattcag tctctcagga atcgcagctc
     3241 tggctcaggg aggcgcgtca ttcaagaaac ttccttacgc gggttatctg gtcacgatca
     3301 gtctactcat cgctgcagac actcttgtga tctatttcat cattggcctt cttttgtttc
     3361 cggctaacgt cttggaccaa agccagctca tccttgcggg aggtttgtcc gcgggcatct
     3421 ccgttacctt ctcagtggcc aatgtgctgc gtcagttcat tggaggttcc gtacttgctg
     3481 atatttttct gggccgctac catcgcgcga aagaggaaat tcgatctttc gttttcattg
     3541 accttgtatc ttccaccgcg cttgcagagt cacttggatc gagtcttttc tttcgcatga
     3601 tcaatgattt ccattcagtt gtagaaagag ctacacgcgc aaacaaaggg attctctata
     3661 aatacatggg agatggacaa atcctggtct ggcccggcac tctcgaagga agaacctccg
     3721 cgatcctcgc atgcctggaa atacaaagag aactggcgga actgcgtgaa cgattcgaaa
     3781 gcaaatacaa tcatgcggcg gacttcaccg ccggtcttca ctgcggcccg gtgctgatcg
     3841 gagaaatcgg agacgaaaga aaagaaattg gatactgggg agacacagtc aacacaactc
     3901 agagaatcca atcatcttgc aagacttacc attgttctat tctcgcatcc gaagcgttca
     3961 tggaccagat ccaatccctg cccgccagac tgtctgtggc gcagaacgtg ccgttacgcg
     4021 gtaaaagcaa tccggtgaac ctgatccgaa tcgaatcact cactgcgagt gagactgact
     4081 cgcatagtta accctgctga cccctcaaat gcacgcacga cttgaccagg atcaagtccg
     4141 gacttgacct ggcggaagtg gactttgacc gggccgttca ggcacaacat acaatggaga
     4201 gtgcgcgcac gtgcgcacct gcgcgggcaa gaacttgcag ttccgctcga agggatagac
     4261 gagtgcttct taaccaggtg gattgattcc agaccggatc ttgcgaacag acctcaatgt
     4321 gtttctctga aacgtgcaca cgaagccctc cacaaaatcg tcgcaaaaaa agattcaggc
     4381 ccggccacga cagacctcct tcaagatttt gaaatccagt cgcgcgcagt gatgacggcc
     4441 atcgaaacca tgcgccaggc aattcgacca tgaacctcga aacacaaccc gcgggagaaa
     4501 acatgtgaaa ctactgattg tagacgactc aaacatcatt cgaaaagcga ttcaaaaata
     4561 cctggccgag ttcgggcttg aaatcgtggg agaggcagga gatgggcgaa ctgctctgac
     4621 tctattcagg gaaacatctc cagatgttgt gacgatggac atcacgatgc cagagatgga
     4681 cggactggaa acactcagcg aaatgcgaaa gatcagcgac tctcctaaaa tcatcattgt
     4741 tacagcactc aaagacaaag caaccgcact cgacgcactc cagaaaggag cgtctggatt
     4801 tctggcaaaa ccgttcacgg aagcacaact caaggaagag atcggccgcg cgatcgggag
     4861 agcatcatga ctcaaaagga tcttcaaatc tttgtaaatg gaaccgtaaa ctactttaag
     4921 cagctgactg attcaggagc gcaaatggac gttcccttcc tgaaaaaacc aggcgaaacc
     4981 gctgcactcg atatcacggg agtgattggg atctcgggag tgaagaaggg gattatctat
     5041 tttactgcgt cctcagatat gctcagggat gtagtcaaga caatcctcgg aactgaaaat
     5101 ccggatctcg catcgattgt tgatatcgcg ggcgaaatcg caaatacgat tgcgggaaac
     5161 gcaagagagg catttggagc ggacttcatg atctccattc cgctcgttgt ggaaggtccg
     5221 ccaaagaata ttcgcatccc gaatgaagtg cccgtgttcg tcattccgat ccggtggaag
     5281 gaccatcgat cttttctagt ggtgggaatc cagtgagcgc gggagcagcc tttccagttg
     5341 tagatacgga tcacgcggtt gaaatcgcgc cagaagtatt ctggaccggg tactacatgg
     5401 aagacgaagc gctccattgc aatccgtatc tcattcgaaa cggaactgaa tccattctga
     5461 ttgatccagg ctctgtacct gacttccctg tagtcggacg aaaactattc tccgtgatca
     5521 aaccagaatc ggtcacaacg atcatactca atcatcagga tcctgatctt tgcgcgtccg
     5581 tgccgatctt tgaaagtctg cgcgagaact ttgactacga tgtaatagcc catcgcaaga
     5641 ctatttattt gctgaagcac tatggaatca aaggaaaatt ccacagactg gacgacgggg
     5701 aaactgcata caaaactccc ggcggcagag agctcaagtt catcacaact cctttcgcgc
     5761 atttcgcggg agcagtaatg acgtacgatg taaaatcaaa agttcttttt acaggagata
     5821 ttctcggcgg agtcggcaca gactgggagc tgtatcatac ggatatagca ctggcaaaca
     5881 tgaaagcgtt tatgcaggga ttcatgccat gcaacaaggc gctccgttat gcgctcttga
     5941 agatagaatc gtccggagca gaactttttg cgccccagca cggacagatc attcatagag
     6001 accaatttcc ctccataaag gaagcgctct gggatctaaa gtgcggactc gacttgatag
     6061 aagacgatgc catgaaaaaa gcgatggttc atgcggtatg acatcgcgcg aagaaatcac
     6121 gcgcgcggag aattattatc gagcaatcag tgaactgacc gtcgccagac atcgcgaaca
     6181 tctcatcaaa gagttgctca actacatggc ttctcttctc aacgccgtcg aagaagctaa
     6241 ctccagactt actttcagtg aggctcgcat ccgggccatt gtcaatactg cagcagacgg
     6301 gattctcacc tacgatgaaa gcggcaacat agacagcatc aaccctgccg ccgccgccat
     6361 gtttggattg aatcctgagt ccatacaggg cctgcacgtt tcctctctcc tcgaaggcga
     6421 atccgaagga cacctgcgga ccgtaaacgc caggcaagtt ggcggcggct acgaagatgt
     6481 ggcaaagagg ggaaacaaca ccttccccgt ctatatcgca gttcaggaca tgcgacttgc
     6541 tgatcgcatg ttccacgtgg ccattctcag agacatcaca gatcagaaga atgcacaacg
     6601 cctcgaggca caggcgagag caaaggaaga gacagacaag atcctttctg ccattgatca
     6661 gggtctgttc ttgatttatc gggatggagc acgatttgaa atcgggtcac agttctccag
     6721 agccacgcga gacattctgg gtgtggacct ggaaagccag aaggacttgc tttccattct
     6781 gggaaacaga gtggctgaaa gcacgattgc ggccgcaaag aatttcctgg agctgatgtt
     6841 caaggattcg gtgcgcgaag aaagccttcg ctctctcaat cctctctctg aaattccatt
     6901 cgcctcccag accgcaggag acacgcgcac gcttcagttt acgtttcgca gagtcacgac
     6961 agaccagaag atcactcact tgatggcaac cgtaagtgac gtaacagagg aagcagcact
     7021 gcgagaacaa ctcaaacaaa acgaacagca gtccagaacg cagatggaaa tgcttttgaa
     7081 gatcctgcac gtagactctg atatgctcga tgaattcata gacgaaactc gtctcgaaat
     7141 cgacatcatt gataccattc taaaaaagga agatggagac ctgcgaaaga gattggagac
     7201 gatctttcgc actgcgcaca agatcaaagg caatgcggat ttactgggtc tttctttctt
     7261 cgcagaacag attcatagct ttgaagacac actgtcggtg ctcttgcgaa atgaggaaat
     7321 ctctcccgct gatttccttc cggtgctttt tgcattcaac aaagtgacgg aaattgtgga
     7381 cgcaattgac gccctggtgg agcgcctgct cgatttcaaa agtagatttg gacagagcaa
     7441 tagcggagat gaattgattc ttcgctcagc ccgggcgctc atcaacaagc tgacggatga
     7501 aacaggtaag ctcgcagaac ttcatgttcg tgtattcgaa gcccgcactc ttggcacgcc
     7561 taaacgcaaa ttgatccgcg atgtcatcac tcagctcatc aagaatagca tgatgcacgg
     7621 aatagaaact ccggaaacgc gcactgccgg cggaaaacga aatccggcaa gaatcgaact
     7681 gaacctgcgt tcaggtaaag atggaatctt cctcagctat aaagacgacg gacagggtct
     7741 tcatctttca cgattgaagg agaaagcaat caagcaggga atcgccacag aggctgaaat
     7801 cgcaacctgg ccacccgcga aaacggcgga actcatattc gccacaggcc tttccacttc
     7861 actgacaact ggaatccaat cgggacgcgg cattggaatg ggtttgatcc gcgaagcagt
     7921 gcacgacgtt cagggtaaga tcagaattct atccgcgcct gcaaagtttt tcgaagtaca
     7981 gatctggatt ccggtcgcat gatgtctttc cagggcgcag cgtctatcct gaacctgact
     8041 gcgaccggaa tgcttttgct ctatgtcgca gttaagttca ttcggagcag aagctatgct
     8101 gcggcacttc cctggcttct gattcgcctg gcaacagctg cagtcacagc gaccaccggc
     8161 ctcatgatcc actggcctcc cattctcatt acgatcatct tcttactact cattctggaa
     8221 ggattggccg ctgcgtttac tgcgaccaga gtcacagtga ccagaccaca tggtagaaga
     8281 cttgcagtgt ccatcggcat cgtttgtatc attgcatcca ttgccatgat tgcttttccg
     8341 gactcgggat acaccgcgtt cccattcatt gtgacagccg cgtggagtgc ggcgttcgta
     8401 tgcctggtgc aaggcggaat cagaaaagaa atccacggat ccctcttctt cgcgcaggga
     8461 ctttcccttg cgggagcgct cctgcatttg tatttactgc gttgcagcca ggaggccatc
     8521 ctcttgattg cagcatctgc acttcagtgc ctgagtttgc tatttatttt ccaacgagtg
     8581 gcggatgctg atctagtaac atcaagtctg aagtcccgtg tattgttctc agggaccatt
     8641 gtctctctca tggtagctct cgcaggcctc ggagcggcgg acgccttcta tcagaaactc
     8701 catgcagtag atagagaaca ccagttagtc gaaatgtcca atgagcttca ggaaaatgat
     8761 ttttcgcaca tgcctgaatc catcgagtac attgtggatt cggagaattc tgaaattgtc
     8821 ttcgctcgca atgttcggat tcagaagatc cccttccgtg aaaccagaag tgacattgta
     8881 aaatgcgaaa catgcaaacc ggagactctt tgcaatctaa tccagtggtc ctctcccaga
     8941 catctgcgga tatttggacc ggatttatcc ttcgacattc gcacggtaaa tattaactct
     9001 catacatacg aagcgggcat tgacgttctg gattacagaa agtacgaaag cgaacgatcg
     9061 acgcgaacct tgctgttctc ttacgcggct ctggtgatcg taaccatggc tctcttcttt
     9121 ttcctcaggc acacggtcct gactccgctc tctattctga tggaaaacct tcagagtctg
     9181 gcaaaaggag aacctacccg gcccatgccg cgagatgaac tgggggcggt aaacaaggtg
     9241 atgacaagac tcatgcatcg cctggcaaaa aataaggacg cactgacaag ggccaacgcg
     9301 caactccaga aggaaaaaaa catactggaa aaattcttct ccaatgattt tgtagaacaa
     9361 cttctacagg agaagatttc gcattcgctt ggaggggcgc gcgtcccggc cacaatcgtc
     9421 tttctggata tccgcaattc aacggcaatc gctgagaa
//
LOCUS       DAORFF010000072         9433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3423_length_9433_cov_3.671678, whole genome shotgun sequence.
ACCESSION   DAORFF010000072 DAORFF010000000
VERSION     DAORFF010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3423_length_9433_cov_3.671678"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKK76_04575"
     CDS             <1..294
                     /locus_tag="PKK76_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67772.1"
                     /translation="DTKLSVADFAKGYEDRIRKETPRAIFDPTENSSGAVRLTSRTDV
                     EGKTVRTVSVFFPGRKSDIVHAYALIPLPDASGASSFSDRWLSGFVWDSSCTY"
     gene            326..1147
                     /locus_tag="PKK76_04580"
     CDS             326..1147
                     /locus_tag="PKK76_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67773.1"
                     /translation="MKNPVPLALTGGLLLAFAFVFLFGQRAQFAGWISKGELAVREEK
                     GTGGPELASLPAPVQTYLGKVLPVKKDIQFVMLKQSGSFWTAPGKDPSSFDAVQYTSV
                     RAPSFSWTAHIQMTPVRVIVVDRLVNGEGALRARVLGLYTVQDASGKEILKGELLRYL
                     AEIPWAPQAILYQKNLFWKPLDATHVELSTNMGDVRAKVVVEFGADGLISRIRAEDRP
                     FKDGDRMIARPWQGMFSDYKEYKGVLLPSHGEVSWILDAGTFKYYSGDLEDYAMY"
     gene            1212..2528
                     /locus_tag="PKK76_04585"
     CDS             1212..2528
                     /locus_tag="PKK76_04585"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNL67774.1"
                     /translation="MQTLTIARFEDLALAQPIQRAVAEEGYTTPTPIQQQAIPHLLEG
                     RDLLGCAQTGTGKTAAFALPILHRLGLAHKKPTPRHTRVLVLVPTRELAVQVHDSFRV
                     YGKHLHIKTAVVYGGVGQGTQVRSLQTGVDILVATPGRLLDLIDQGYLRLDTLEVFVL
                     DEADRMLDMGFIQPIRQVIEMLPPRRHNLFFSATMPPPIQKLAGSMLVNPVRVEVTPV
                     ASTVEIIDQSVMYVEHDRKRDLLVKLFQDKSLSRVIVFTRTKHGANRVAEMLDKSGTP
                     ADAIHGNKSQSARQRALENFRVGKIRALVATDLAARGIDVDGVTHVINFEIPNESESY
                     VHRIGRTARAGASGKAISLCDDEERAYMKDIERLINKKIRVDESQPYHSDRIANLTNT
                     PRPAPARKGGGGHGHARGKNHPRSSNGRAGSGSSGNRSSRNLGRRR"
     gene            complement(2570..3487)
                     /locus_tag="PKK76_04590"
     CDS             complement(2570..3487)
                     /locus_tag="PKK76_04590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNL67775.1"
                     /translation="MSLVALIKPKGPSGFGYGTTAEEVTQGLDLSGKNILVTGCNSGL
                     GEETARVLAKRGARILGTARTVEKANAAVAPLGNASTGFECELSDPKSVRSCVDAIKK
                     DGVKLDALICNAGIMALPELKQASGYELQFFTNHIGHFILVNGLLDQLKSNGRVVMLS
                     SSAHANAPREGIQFDNLSGEKSYRPWAFYGQSKFANLLFAKELARRFAGTERTANALH
                     PGVIKTNLARHMNPLAHVAFGVIGPVFLKSIPQGAATQTYVAVHPHAAAHSGKYFADC
                     NIASPRRDAEDADMARKLWDVSEKIVSGL"
     gene            3574..4341
                     /locus_tag="PKK76_04595"
     CDS             3574..4341
                     /locus_tag="PKK76_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67776.1"
                     /translation="MRSSLQLYSILSGLAAILLGIGIALSGFSGEVQEWIFLCALALW
                     HGIYAIWFWKGKELIAFARITVVGRAFSGISYWLVLGLLPDAEGSLMILFRTYLTVQG
                     SIDLGSAAITAFSVLQKKEADGKDRSLSLEEKNRFVFAIYMTLLSVWILADSFSFLKF
                     FHLPPTSFSGWTAFRFGPIHILAAQIFILAIYNFTAVRFRLTPLIEAGMRGGMVTCIF
                     FVILVLFGVLHPITLLLPGADLISVAVIFFARLRKRQ"
     gene            complement(4343..6157)
                     /locus_tag="PKK76_04600"
     CDS             complement(4343..6157)
                     /locus_tag="PKK76_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNL67777.1"
                     /translation="MIREKFEEIRKSLGNLPGAFRIVWEADSGSTIRLASLTVIAALL
                     PVTQAYIGKLIVDSVVKAVSTHTPGIEALREILPYLLTELALFAAGSFLAQTRRLAEQ
                     VLDQRAGHHITEQIIRKALTLEIRYFEDSKFYDNMQNARREAEYKALAIINNSFLLVQ
                     NTITLFSFVFILFAMSPIIALVLFGATIPSFAVQTRFSKMKFRMQSWRSPETRMITYL
                     EQILTMNTTVKEIRLFGLGDELLNRFNSIFHKIFTEDMSLAKRRLLFSYLWGLLSTLS
                     FYGCYAWIIYLTVLQRITLGDMTLYLGAVRQSQGSFQGILDNVSRLYENGLFMQNLFS
                     FLSLPGMNPTPSSKNWPATDTPGIHFDNVTYRYPDADLPALENFSLHIKPGERFAIVG
                     ENGSGKTTLIKLLSRLYEPADGAIRLDGVDLRDMSLDQIYARMGVIFQDFVRYQLSVR
                     ENIGFGDIAHLSETERILGAVHDAGADSLLADMPEGLETSLGHQFRRGRELSGGEWQK
                     IALARSFMRSAEILILDEPTSALDAQKEFEIFERFRSLTAGRTAILISHRFSTVRMAD
                     RIAVLEHGKLVELGSHDELMKLGGKYARLFELQARGYR"
     gene            complement(6167..8476)
                     /locus_tag="PKK76_04605"
     CDS             complement(6167..8476)
                     /locus_tag="PKK76_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL67778.1"
                     /translation="MSSRQLHWVIIAVTGLAFCVSPAPKQPASSVTSRLVVVAPPRNA
                     PDDLSAGIRSILEALLQSAGMPVLPSSGVAGLTDNALEPALMCARAGAQTCIVPEIAS
                     QGTGISIRIRWLDARGEVTSSTFNVTLRETSQGIQDAFVSMMDAARAKGWLETSDWSA
                     DRQLAASRMIRPQTETLQHFSRGLSLESTLPYMAMLEYRNALLIDPDFTLAYARLFYV
                     CYESNPYSQTAEVDLLTQTRVRMDTDNAVWLALAFRDLGQNALRKGWLITSDAYFRRS
                     LAILANIKKETSIPGSIALHGLGDLGLKNRNSINALYHTNSAQQALESVGAQNSSLYL
                     SGLLRLGAGYLLDNQTSLAVYTYEKARDLAGRLNAADSPEAAIIDANLGLARIRESNY
                     VGAAALYESALARLRKTGYVNTELYLTILGHLGQAQRFMGNISEAEATFERVITGSKI
                     LGLNESMIQAEAMYSTGALSMVRGDFARAQVLMISAQMTMMRLGNQRNAADHVALDLM
                     PVKQQAGMTLEEARLLSSYCGAFSYASHSRTVQARTYEGRLDDTNVFLRDLLDVRKSN
                     DAALNHLREKFATVPDPSGRDTAFVDIGPAIANMLTPGVTAVSLARDFPGMQVVALDL
                     PEQVEIFRTTVAAYLRSRVTAFPNMFILSGNGIQPMMDQFQNNANWFSQKQPRIPAGM
                     PLVIRAANSVDIYESWETNAKAMDRIAKDFKASAILYLFNRSILFKPAGSDSFRIIGV
                     LSPAGFDHMFETFNRQGDSAYTIFPDAVR"
     gene            complement(8530..>9433)
                     /locus_tag="PKK76_04610"
     CDS             complement(8530..>9433)
                     /locus_tag="PKK76_04610"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL67779.1"
                     /translation="EPRQQVELAVLGLVDEMTGLMYYVNAEHPWTVLYRNGRAQFIEE
                     ELTFRKLGTQGLEGGMSIQTFQLQPGDILIAGSDGRDDLLLNTKEDGERVISEDEHAF
                     LKHVEAGKGELRKIYDSIKTVGELTDDFSLVRIGYLETAKQTRTSSEKAMEFVSKART
                     FLIQDNLDEAQIALEEAVKLDNTDTEALKELIKVCYNKRDFRKASDYADDYIYLRPAE
                     SEILYLAAICHKLAGELARSADLAESLKLREPKRPEVLTLLAQIYLEMNNIPRARAMV
                     DLALSLDPASAAALKAHHEIQSPR"
BASE COUNT         2155 a         2379 c         2427 g         2472 t
ORIGIN      
        1 gatactaagt tgagtgtagc ggactttgcg aagggctacg aagatcgcat tcgcaaagaa
       61 acaccccggg caatcttcga ccctacagaa aattcctccg gggcagtgcg cctaacgtcc
      121 agaacagacg tagaaggaaa aacagttcgc actgtttctg tcttttttcc gggcagaaaa
      181 tcggacattg tgcacgctta cgcattgata cctctgcccg atgcctcggg cgcttcctct
      241 ttttctgatc gatggctttc tggttttgta tgggactctt cgtgtacgta ttgacgttcg
      301 cacggcgcat tccctttgtt gcggtatgaa gaatccagtt cctcttgcgc tcacaggtgg
      361 tctattgctc gcgttcgctt ttgttttctt gtttgggcag agagcgcaat ttgctggatg
      421 gatttcgaaa ggtgagctgg ctgttcgcga ggaaaaggga acaggtgggc cggaactggc
      481 ttctctgccc gcgcccgtgc aaacatattt aggcaaagtt cttccggtaa agaaggacat
      541 tcagtttgtg atgctcaaac aatccggttc cttctggact gctcctggaa aagatccctc
      601 ctcttttgat gcagtccagt acacttcggt gcgtgcccct tccttttcgt ggacagccca
      661 catccagatg actcctgtgc gtgtgatcgt tgtggatcga ctggtcaacg gagaaggggc
      721 tcttcgcgcg cgcgtacttg gactctatac tgtgcaggat gcttctggaa aagaaatcct
      781 taaaggtgag cttctacgat accttgcaga aattccctgg gctccccagg caattctgta
      841 tcaaaagaac ttattctgga agccactgga tgccactcat gtggagctca gcacaaatat
      901 gggcgacgtc agggcaaagg tcgtggtcga gtttggtgcg gatggactca tttcgcgcat
      961 tcgtgcggag gatcgtccct tcaaagatgg ggatcgcatg attgccaggc cgtggcaggg
     1021 aatgttctca gactacaagg aatacaaggg agtcttgctt ccatcacatg gagaagtgag
     1081 ctggattctg gatgcgggaa cattcaaata ctacagcgga gacctggaag actacgctat
     1141 gtattgattt agatatttga atatctagta tccgccaccg gcttccgcga aaaactgact
     1201 caaactactt catgcaaaca ctaaccatcg cgaggtttga agatctcgca ctcgcgcagc
     1261 caattcagcg cgcggtagcc gaagaaggct acactactcc aacacccatc cagcaacagg
     1321 ccatcccgca tctactagaa ggtcgtgatt tgctaggttg cgcgcagacc ggcactggca
     1381 aaacagcagc gtttgctctt ccaatcctgc atcgtcttgg tctggctcat aagaaaccca
     1441 caccgcgtca tactcgcgtc ctggttcttg tgcccacacg agaacttgcc gtgcaagtcc
     1501 atgatagctt ccgagtttac ggaaagcatc ttcacatcaa aactgcagtc gtttatggcg
     1561 gcgtgggaca gggcacccag gtgcgcagct tgcagacggg cgtggacatt cttgtcgcaa
     1621 cacccggcag actccttgat ttgattgatc agggttatct tcgcctcgat actcttgagg
     1681 tctttgtcct ggatgaagcg gacagaatgc tcgacatggg tttcatccag cccattcgtc
     1741 aggtgattga gatgcttccg ccgcgccgtc acaatctttt cttttctgcc accatgccac
     1801 ctcccatcca gaaactggca ggcagcatgc ttgtaaatcc agtgagagtg gaagtgaccc
     1861 ctgttgcatc cactgtagag atcattgatc aatccgtcat gtatgtggag cacgatcgca
     1921 aacgagatct ccttgtgaag ttgtttcagg acaagtctct cagtcgcgtg atcgtgttca
     1981 cgagaaccaa acacggtgcg aatcgagtcg cagagatgct cgataaaagt ggaacgccgg
     2041 cagatgcaat tcacggaaac aagtcgcaga gcgctcgtca gcgtgccctt gagaattttc
     2101 gcgttggtaa gattcgcgct cttgtggcca cagacctggc cgcgcgcggt atagacgtgg
     2161 acggagtaac tcacgtaatc aatttcgaaa ttccaaacga atcagaaagc tatgttcata
     2221 gaatcggtcg aaccgctcgt gctggagcgt ctggcaaagc aatctccctg tgcgatgatg
     2281 aagagcgcgc ctacatgaaa gacattgagc gtttgatcaa caagaagatc cgcgtggacg
     2341 agagccagcc atatcattcc gatcgaattg caaatcttac aaacacacct cgccctgccc
     2401 ctgcaagaaa gggtgggggc ggacacggcc atgcgcgtgg caagaaccat cctcgttcga
     2461 gcaatggcag agcgggttct ggttcctcag gcaaccgctc ttcgcgcaat ttgggaagaa
     2521 gacgttaggc gccatttacg gcgcctgatc aaataacgtt acgcggcatt cacaggccgc
     2581 ttacgatctt ctcggaaaca tcccacagct ttcgcgccat atccgcatcc tctgcgtcgc
     2641 gtcttgggct ggctatgttg cagtctgcga agtatttgcc tgagtgggct gcggcatgtg
     2701 gatgaacagc aacatacgtc tgtgtggccg ctccctgggg tatgcttttg agaaaaacag
     2761 gtccgatcac tccaaacgct acgtgcgcca gcgggttcat gtgtcgcgcc agatttgtct
     2821 tgatcacacc gggatggagc gcattggcag tgcgttctgt tcctgcaaac cgacgggcga
     2881 gttcttttgc aaagagcaga tttgcaaact tggattggcc atagaatgcc cacggtctgt
     2941 aggatttttc tccgctcaaa ttgtcgaatt gaatgccttc gcgaggcgcg ttggcatgtg
     3001 ctgaactact gagcatcacg actcttccgt tgctcttcaa ttgatccagt agcccgttta
     3061 caagaatgaa gtgaccaatg tggtttgtga aaaactgcaa ttcatagcct gacgcttgtt
     3121 tcagttccgg cagtgccatg atcccggcat tgcaaatcaa cgcgtccagt ttcactccgt
     3181 ctttcttgat ggcatccacg caagatcgca cgctctttgg atccgaaagt tcacattcaa
     3241 atccagtaga agcatttccg agtggtgcga cggctgcgtt tgctttttca accgtgcgcg
     3301 cagtgccaag gatgcgtgct ccccgttttg ccagaactct ggcggtttct tctccgagac
     3361 ctgaattgca gcctgtgact agaatgtttt ttccagacag gtctaatccc tgggttactt
     3421 cctctgctgt ggttccataa ccgaacccgc ttgggccttt tggtttgatg agggcgacga
     3481 gagacatacg aactcctttt tcatggttaa ctgcactcag ggaagtaatg cgaaagatca
     3541 tgacgaggcg agcaataact gcaaaattgg accatgagat catctcttca gctgtacagt
     3601 attctgagcg ggcttgcggc gattctgctc ggaatcggca ttgcactgtc cggtttttcg
     3661 ggtgaagtgc aggaatggat ctttctctgt gctctggctc tctggcatgg aatctacgcc
     3721 atctggttct ggaaaggaaa ggaactcatt gcatttgcgc gcatcacagt tgtgggtcgc
     3781 gcgttttcgg gtatttccta ctggcttgtg cttggcctcc ttccagacgc ggaaggcagt
     3841 ttgatgattc tgtttcgcac gtatttgact gtgcagggca gcatagacct tggaagtgct
     3901 gcgatcaccg cgttttctgt tctccagaag aaggaagcgg atggaaaaga tcgttcgcta
     3961 tctcttgagg agaagaatcg atttgttttc gcgatctaca tgactttgct ctccgtgtgg
     4021 attcttgcag acagcttttc ttttctgaaa ttctttcatc ttccgcctac ttcattttct
     4081 ggttggactg cgtttcgatt tggtcccatt cacatcctgg cggcgcaaat tttcatcctt
     4141 gcgatttaca atttcaccgc tgttcgcttc aggctgacgc ccctcattga agcgggcatg
     4201 agaggcggaa tggttacttg catttttttt gtaatccttg tgcttttcgg agtgcttcat
     4261 ccaatcactc ttcttttgcc tggagccgat ttgatttccg tggccgtgat cttttttgcg
     4321 cgactgcgaa aacgacagta agctacctgt atcctctggc ttgaagttca aatagacgcg
     4381 catacttacc gccgagtttc atgagttcat cgtgacttcc cagttctacg agtttgccat
     4441 gttcgagcac tgcgatgcga tccgccatcc gcacagtgct gaatctatgc gaaatcaaaa
     4501 tggccgttcg acctgcagtg agggatctga atcgttcaaa gatctcaaac tccttttgcg
     4561 catcgagcgc cgaagttggc tcgtccagaa ttagtatttc cgcgcttcgc ataaaagatc
     4621 tggccagcgc gatcttctgc cattcgccgc ctgaaagctc tctacctcga cggaactgat
     4681 gtcccagaga tgtttccagt ccttcaggca tatctgccag aaggctgtct gctcccgcgt
     4741 catgaactgc ccccaggatt ctttctgttt ccgaaagatg cgcgatatct ccaaagccaa
     4801 tgttttctct cacggataac tggtaacgca cgaagtcctg gaagatgact cccattcgcg
     4861 catagatttg atccaggctc atatcgcgaa gatccactcc gtcgagtcgg atcgctccat
     4921 ctgcaggttc gtacaggcga gatagcagct tgatcagggt tgtcttaccg cttccatttt
     4981 cgcctacaat cgcaaagcgt tcgcctggtt tgatgtgaag gctgaagttt tccagcgcgg
     5041 gtagatctgc gtcgggataa cgataggtga cattgtcaaa gtgaattcca ggcgtgtccg
     5101 tggccggcca gttcttgctc gatggtgtag gattcattcc tggtaggctg aggaacgaaa
     5161 atagattctg catgaagagg ccgttctcat acaacctgct tacgttgtcc aggatgccct
     5221 ggaagctgcc ctgggattgt cgcacggctc caagatagag cgtcatatca cccagagtga
     5281 tcctctggag taccgtgaga taaatgatcc atgcgtagca tccataaaaa cttaaggtgg
     5341 aaaggaggcc ccacaggtag gaaaacaaaa gcctcctttt cgcaagagac atgtcttccg
     5401 tgaagatttt gtggaatatg ctgttgaacc gattcaacaa ctcatcgccc aggccgaaca
     5461 atcgaatctc cttcacagtt gtgttcatgg tcagtatctg ttccagatag gtgatcattc
     5521 tggtttcagg ggaccgccag ctttgcattc ggaatttcat tttgctgaag cgagtctgga
     5581 ctgcaaaaga aggaatggta gcaccaaaca gcactaaggc gatgatcgga ctcatcgcga
     5641 aaagaataaa cacgaacgaa aacagagtga ttgtattttg aaccagcaag aaggaattgt
     5701 tgattatggc gagagccttg tattctgctt ccctccttgc attttgcatg ttatcataaa
     5761 atttggaatc ctcgaagtag cgaatctcga gcgtcagcgc tttgcggatg atttgctctg
     5821 tgatgtgatg accggccctc tggtccagga cctgctccgc gagtcttctt gtctgtgcga
     5881 gaaaagatcc agccgcgaag agtgcgagct ccgtaagcag gtatggaaga atctctcgaa
     5941 gagcttcgat acccggagtg tgggtcgaca ctgcctttac aacactgtct acaatgagtt
     6001 tgccaatgta ggcctgcgtt acgggaagga gcgctgcaat gactgtaaga gatgcaaggc
     6061 gaatcgtact gcctgagtct gcttcccaga caatgcggaa tgcgcccggc aaattcccga
     6121 gggacttgcg gatttcttcg aacttttcgc ggatcaacag attgccttat cgcactgcgt
     6181 caggaaagat cgtgtaagcg gaatctccct ggcgattgaa cgtttcaaac atgtgatcaa
     6241 atccggcagg agaaagtact ccaatgattc tgaatgaatc agagcctgct ggtttgaata
     6301 aaatggacct attgaaaaga tagaggatgg cagaagcttt aaaatcttta gcaattctgt
     6361 ccatggcctt cgcgtttgtt tcccagcttt catagatatc cacggaattg gctgctcgaa
     6421 tcacaagagg catgccagct ggaattcgag gttgcttctg cgaaaaccag ttggcattgt
     6481 tctggaattg atccatcatc ggctgtattc cgttccctga tagtataaac atattcggga
     6541 aggccgtcac tctggatcga agataagctg caacagtggt gcgaaatatt tccacttgct
     6601 ccggaaggtc gagtgcgact acttgcatgc ctggaaagtc gcgcgctaga gagacagctg
     6661 tgactccggg agttagcata ttggcaatgg ctggcccaat gtccacaaac gctgtgtcgc
     6721 gaccggatgg atccggaaca gtcgcaaatt tttcgcgcag atgattcaaa gctgcgtcgt
     6781 tggattttcg cacatcgagt agatctctaa ggaagacgtt tgtgtcatcg agtcttcctt
     6841 cataagtcct ggcctgcact gttcgtgagt gagacgcgta gctaaatgca ccgcaatagg
     6901 aagaaagaag gcgtgcttct tccagtgtca ttcccgcctg ttgtttgact ggcattaaat
     6961 caagcgcaac atgatctgcc gcattccttt gattgcccag tctcatcatg gtcatctgtg
     7021 cggatatcat caggacctgg gcccgggcaa agtcaccgcg gaccatggac agagcgccgg
     7081 tcgaatacat tgcttctgcc tggatcatgg actcattcaa tccaagaatt ttgctgcctg
     7141 taatgacacg ctcaaaagtt gcctccgctt ccgatatgtt gcccatgaat ctctgagcct
     7201 gacccagatg tccaaggatc gtcagataca attcagtgtt cacgtatcct gttttgcgaa
     7261 gacgcgccag tgcggactca tacagtgcgg ccgctccgac ataattggat tctcgtatgc
     7321 gtgcaagtcc cagattcgca tcgatgatgg cagcctccgg gctatccgcc gcgttcagtc
     7381 gtcctgccag atcgcgcgcc ttttcgtatg tatatacagc gagggatgtt tgattgtcca
     7441 gtagatagcc cgcgccaagc ctgaggagac cggacaaata cagagatgaa ttctgcgcgc
     7501 caacactttc cagcgcctgc tgcgcgctgt tcgtatggta gagagcgttg atgctgtttc
     7561 tatttttcag accaagatca cctaatccat gtaaggcgat tgatcccggg atagaagttt
     7621 ctttcttaat gtttgcaagg atggcaagag agcgtcgaaa gtaagcgtcg gaagtgatca
     7681 gccatccctt tctcagtgcg ttttgaccca gatcgcggaa ggccagagca agccacactg
     7741 cattatccgt atccattcgc actctagtct gagtgagtag atcgacttcc gctgtttgag
     7801 agtaaggatt tgattcatag caaacgtaga acagtctcgc ataggcaaga gtaaaatccg
     7861 gatcaatcaa aagcgcgttg cgatactcga gcatggccat gtaaggcaga gtagattcga
     7921 gtgacaggcc tctggaaaaa tgttgcagcg tctccgtctg tgggcgaatc atgcgcgaag
     7981 cggccagctg tctatccgcg gaccagtctg atgtttcgag ccaacccttt gcccgggctg
     8041 cgtccatcat ggatacaaac gcgtcctgaa tcccctgcga agtttcgcgc aacgttacgt
     8101 tgaatgtgct ggaagtgact tctcctcgtg cgtcgagcca tcgaatccga atagagattc
     8161 ctgtgccctg cgaggcaatt tccgggacaa tgcaagtctg cgcgcccgcg cgagcgcaca
     8221 tcagtgctgg ttcgagcgca ttgtctgtga gtcctgccac tccgctcgaa gggagaactg
     8281 gcattcctgc tgattgaaga agagcttcga ggatagagcg aatgcctgcg gataaatcgt
     8341 caggtgcgtt tcgcggagga gcaacgacta cgaggcgcga tgtgacagaa gaagctggct
     8401 gctttggtgc cggtgaaacg cagaacgcca gaccagtcac cgcgatgatc acccaatgaa
     8461 gttgccgcga tgacattcac ccttgtgcgc atatcggtca ggattcgtca cctgttttgc
     8521 aaatcgcaat taacgcggag actgaatctc atggtgcgcc ttgagagccg ccgcactggc
     8581 cggatccagg ctgagtgcca ggtctaccat tgctcttgcg cgcgggatgt tgttcatttc
     8641 caggtaaatc tgagcgagga gtgttagaac ttccggcctt tttggttcgc gcagtttcag
     8701 actttctgca agatccgcag atcgtgcgag ttctcctgca agcttgtgac agatggccgc
     8761 caggtagagt atctccgatt cagcgggacg aagataaata taatcgtctg cgtagtcgga
     8821 tgccttgcgg aagtctcttt tgttgtagca aacttttatc aattctttga gcgcttccgt
     8881 atctgtgtta tccagcttta ccgcttcttc cagggcgatt tgcgcttcgt ccaggttgtc
     8941 ctggatgagg aaggttctgg ctttcgaaac gaattccatt gccttttcag aactcgtgcg
     9001 tgtttgcttc gcagtctcca gatagccaat tcgcacgaga gaaaagtcgt ccgttaattc
     9061 acccaccgtt ttgattgaat cgtagatttt tctcagttcc ccttttcccg cttccacgtg
     9121 tttcaagaaa gcatgttcgt cttcactgat gactctttct ccgtcctctt tcgtgttgag
     9181 cagtaggtcg tctcttccgt ctgagccagc aatcagaatg tcccccggtt ggagttgaaa
     9241 cgtctggata gacattcccc cttcgagtcc ttgcgtgccc agtttccgga atgtgagctc
     9301 ttcctcgatg aattgcgcgc gtccatttct gtacagcacg gtccatggat gttccgcatt
     9361 tacgtagtac ataagacctg tcatttcgtc cacgagaccc agcacggcaa gctcaacctg
     9421 ctgtcgtggc tcg
//
LOCUS       DAORFF010000073         9423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3430_length_9423_cov_3.682323, whole genome shotgun sequence.
ACCESSION   DAORFF010000073 DAORFF010000000
VERSION     DAORFF010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9423
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3430_length_9423_cov_3.682323"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2480)
                     /locus_tag="PKK76_04615"
     CDS             complement(<1..2480)
                     /locus_tag="PKK76_04615"
                     /inference="COORDINATES: protein motif:HMM:NF021424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNL67780.1"
                     /translation="MAIRVALHHKTSYRYDHAVTAFPHVVRLKPAVHSRTPILSYSFR
                     VGPEPHFINWQQDPFGNHLARLVFPEKTDRLDFTVDLVAELHSINPFDFFLEPDAEQF
                     PFQYSPDVKAQLAPYLALRENGPLLKYYVESNRRKQKTIDFLVELNQRIHSDVSYLIR
                     MEPGVQTCEDTLKLKSGSCRDSAHLLVHVLRGLGLAARFVSGYLIQLKPDETPLSGPP
                     GPAHDFTDLHAWAEVYLPGAGWVGLDATSGLFTSEGHIPLACSPNPEDAAAITGAVEP
                     AKSDFSFEMKVTRIEESPRVSQPYTDLQWKKIQGVGRVVDADLMSHNVRLTSGGEPTF
                     VSQTDRDAPEWNISANGLKKKDLARRLALALRGRFAPGSSFHSGQGKWYPGEEIPRWA
                     YSVFFRKDGRPLWRDDRLLADETRNYNYGPREARMFLEALATRLVGTTETIVPGYEDA
                     YYYLWKEGNLPADVDPMRKDLSSESERNRLRILLERGMGSITGFALPLNFDGSLQSWT
                     TGSWHFRRERMYLIPGDSAMGYRLPLESLSGTDIGFPELSPMQEKSPFPDVAARTLRR
                     AQTILATSGDSSSQTQIPMIHMRTSVCVETRDGRLYVFLSPQWDAANYVDLIASVEDT
                     AAELQMPVLIEGYEPPTDSRLGRIAVTPDPGVVEVNIHPSDSFEEHARKLEILHEEAR
                     KCGLTTEKFLHDGRRSGTGGGNHITLGGPTPMESPFLQRPDLLRSLITYFQHHPALSY
                     FFSGQFIGATSQSPRIDETNPESLYELAIAFSQLPRSAVPVPGDAAPWLVDRLLRNLL
                     VDQMGNTHRAEISIDKLHSPDSLTGRLG"
     gene            2819..3094
                     /locus_tag="PKK76_04620"
     CDS             2819..3094
                     /locus_tag="PKK76_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HNL67781.1"
                     /translation="MELIHALATSLLSPMVRCSVFEVGLVTGRRMQDLKKVGWFLIAF
                     AVVMPLIHGLPGVLIGTWTGLSIGGTCVARISHHLPPDSFYCSHFSF"
     gene            3143..3451
                     /locus_tag="PKK76_04625"
     CDS             3143..3451
                     /locus_tag="PKK76_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67782.1"
                     /translation="MNLHHVKLLTIIAPDNLEARLTSDVRLFGARGYTISPAVGAGLS
                     SVRDNEWTGKNIRLETLVGQDVAYALMAHLAKDYFEKFGVIAFMSDVEVLRKERFDYR
                     "
     gene            complement(3448..4014)
                     /locus_tag="PKK76_04630"
     CDS             complement(3448..4014)
                     /locus_tag="PKK76_04630"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNL67783.1"
                     /translation="MSFSALFSVRSVGIAFLALMISSSAQAQGRAPEHLTDSAQIAMF
                     NRLTSKVNCYCGCHSIIGTCGHIEENCFGVQLRRFIENRIIEGRSESSIEEGLVSGFG
                     EEIRKDPQVIILQAEGRVDLVSGFVKGFGKMIFVKPPVVWPWIVVAIVGAFALLWVWS
                     RRNKPRPVAHARSPEIDSILNRTRELDR"
     gene            4125..6701
                     /locus_tag="PKK76_04635"
     CDS             4125..6701
                     /locus_tag="PKK76_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNL67784.1"
                     /translation="MGLTVAIVLGLLLAVCAAWIARELRNRRNQIARQLFLRHGSVML
                     LIKPDDGRIAFANLAAARFYGYSPRQLESMSVKDLNTLGPAEVQAELDRARREERSYF
                     LFRHRVASGDIRTVQVHSSPLVIDGETFLLSIIDDVTHHAKLTKERETRGRVLELLAA
                     NAPLESILTELVLAYEDLAPGSICSILLTDSEESTLLPGAAPGLPEWFNHEVQGLSTG
                     EGQGSCGTAIARKQRVIVSNIATDPLWDKYRHLAERAGLAACWSEPIFSGSRKILGSF
                     AIYYKQVREPHEEDLQLISRHAQLASLCIEQTQLRRRQQLADHVFQNTGEGILITDDK
                     SQILAANPAFGKLTGYEPAEILGKTPRILSSGKHPKSFYEDMWGQIRNTGKWKGEIWN
                     KRKNGELYPELLSISTVYTKGSVIGYYVAIFTDISNIKETEARLDELAYRDPITALAN
                     RSLFQLRLAEALHRAERSGTSVWILLIDLDRFKDVNDALGHEVGNKLLEAVTGRFLEV
                     LEPGDLLARMGGDEFGLLLENADAGRIAQRLLHSLNAPVHLKSGDQIDVGASVGISQF
                     PHDGNSVEQLIQNADAALYDAKASGRRTFRFYTSTLTARAKARLSLETKLRRALERNE
                     ILLHYQPQVNIRTGKIVGVEALARWPDGDQMIPPDQFVPVAEESGLILPLGQSLFRKA
                     CMQARQWNDAGISLMVAVNLSARQCANARLAHEIAEVIRETGVNPEQLELEITETALM
                     SQGQDALNLLEQMRKIGVRMALDDFGTGYSSLAYLRHFPVDTLKIDRSFISEIPTHNS
                     GAEITSAIIRLGHSLNFTVLAEGVETEAQLAFLAKQECDLYQGYYRGRAVPPEEIPAL
                     LQ"
     gene            6784..7833
                     /locus_tag="PKK76_04640"
     CDS             6784..7833
                     /locus_tag="PKK76_04640"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004433704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNL67785.1"
                     /translation="MEPRLTLPAVRDTISKESLRNPTWKGLLYFARDIAVYAAILVGL
                     YSTDAWYFLVPLWILAGLAVSGLFIIGHDAAHGALFKNQKLAYLIGQISMLPSLHAYH
                     QWVYGHNRIHHGHTIKMEGDFVWRPTSPAQYRDLSWAGKIWHRITWSAIGSGIYYLVQ
                     IWFGGMILFVAQTKGAKRDRILIILAAIAMIAVPIALFPTWQAGLWVAVKLGVIPFLA
                     FNYCIGFSVYVHHIHDFIPWKKHKDWTPFYGQMLGTVNYHIPAFFNFFLHNIFLHMPH
                     HVNMRVPFYNLGRALSEIKSVYGQYVIERKSMFMDYLHTTRKCKLFDPESGKWLTYAE
                     AAEERTPNLSQSVPA"
     gene            7767..8396
                     /locus_tag="PKK76_04645"
     CDS             7767..8396
                     /locus_tag="PKK76_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNL67786.1"
                     /translation="MANVRGSGGRKNAKPQPVRAGLSRESILDAALEILNEGGMAALS
                     LRSIVKRLGCSVAGPYTYFAGQDEIISTLIQRGEEELTGDLKRAGQEGGDLFEVLRRL
                     AATYMTFARRNRELHKLMFNINGHKRIFPSATASYRAYIETIRGGVKSGRLHFSRKGY
                     HAIARTMWAWLYGLIVLEMTGVTTMNADEAIDEGFSFFQRLLEQGEKSA"
     gene            complement(8393..>9423)
                     /locus_tag="PKK76_04650"
     CDS             complement(8393..>9423)
                     /locus_tag="PKK76_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67787.1"
                     /translation="AMRWLLRMARWPGGIERVFLGRAPDTDPLKTCYVWSRKSALDEL
                     ALIHFLLNRPMRRGSFVAKNWPREPFASPHKVSGVVSRLGEASSLFSSLSRFFPEAPD
                     TAEDPAADDALDIPDDSNLAETILCADQEPHLRGRIREPSMPDNVARDYSLKLVPLSV
                     TGDFLCPDLLGRSDLWVHPGSPIHPPGNTAEKPHLNHLLRMSLQKGLPLLATHLISIA
                     LFEFRLEHKPFRMDKVVTRFHDLARRVRRLDFPHDERLDHNEFRNSRMNRFLRRFEKW
                     FYLVDSKYFFKTLEYPEVELGRPMNYFWYADQASHAFYEFGLSKTDLDQFLSRETKAL
                     VVVNPQPR"
BASE COUNT         2437 a         2460 c         2337 g         2189 t
ORIGIN      
        1 atcccaagcc gaccagtcag cgaatcgggt gaatgcaact tatcaatgct gatctctgct
       61 cgatgagtat ttcccatttg atccacgagc agattgcgaa ggagtctatc cacaagccac
      121 ggtgcggcat ctccgggaac agggactgcg gaacgaggaa gctgactgaa tgcgatggcg
      181 agttcgtaca gactctccgg attggtttca tcgattcgcg gcgattgact cgtcgcgcca
      241 atgaactggc cggagaaaaa gtaggaaagt gcgggatggt gttgaaagta cgtgatcagg
      301 cttcgcagca gatcgggcct ctgtagaaat ggactctcca tcggagtcgg cccacccagg
      361 gtgatgtggt ttcctccgcc cgtgcctgat cttcttccgt cgtgtaaaaa cttttccgta
      421 gtcaggccac acttgcgcgc ctcctcatga aggatctcca actttcgcgc gtgttcctca
      481 aaggaatcag atggatgtat gttgacttcc actacgccgg gatctggagt caccgcaatt
      541 cgaccaagac gagagtcagt tggtggctcg tagccctcta tcaaaacggg catttgcaat
      601 tcagcagcag tgtcctcaac gcttgcgatc aaatctacgt agtttgccgc gtcccattgg
      661 ggagacagaa atacgtacag ccttccgtct cgtgtttcaa cacagacgga tgttctcata
      721 tgtatcattg gaatctgagt ctgcgaagat gaatcgcccg atgtagcgag aatggtctgt
      781 gctcgacgta gcgttctcgc tgccacatca ggaaacggcg acttctcttg catgggagaa
      841 agctccggaa agccaatgtc ggtgccggat agactttcca gaggaagtct atagcccatc
      901 gcagaatcgc ccggaatcag atacatcctt tctcttctaa aatgccagct gccggtagtc
      961 catgattgca gtgaaccgtc aaaattcagc ggcaaagcaa agccagtgat gctgcccatt
     1021 cctctttcga gcaaaatgcg aaggcgattt ctttcggatt cagaactcaa atcctttcgc
     1081 attggatcta catccgccgg cagattccct tctttccaca aatagtagta cgcatcttca
     1141 tagcccggaa caatcgtttc ggtggtgcct acgagtcttg tggcgagagc ctcaaggaac
     1201 attcttgctt ccctcgggcc gtaattgtag tttctggttt catccgcgag caaacgatca
     1261 tcgcgccaca gtggccttcc atctttgcgg aaaaaaacag aataagccca gcgcggaatc
     1321 tcttctcccg gataccattt gccctggccg gaatgaaaag atgacccggg ggcaaagcga
     1381 cctctgaggg caagagcgag acgacgcgcc agatctttct tcttcaagcc gttcgcggaa
     1441 atattccatt caggtgcgtc cctgtctgtt tgcgatacga acgtaggttc tccgcctgac
     1501 gttagacgaa cgttatgcga cattaaatct gcgtctacga cacgcccaac tccctgaatc
     1561 ttcttccact gcaaatcggt gtagggttgc gaaacccgag gtgactcttc gattcgcgtg
     1621 accttcattt cgaagctaaa atcggatttc gcaggctcga ccgcacccgt gatagcagct
     1681 gcgtcttctg gattgggaga gcaggccaga ggaatatgac cctccgaagt aaagagcccg
     1741 gaagttgcgt ccagaccaac ccagccagca ccaggcaagt acacttccgc ccaggcatgc
     1801 aagtctgtaa aatcgtgcgc aggtccgggc ggtccagata gtggcgtttc atctggcttc
     1861 agctgaatca aataaccgga aacaaatcgt gcggccaggc caaggccgcg caacacgtgc
     1921 accagcaaat gagccgagtc acgacacgaa ccactcttca actttagagt atcttcgcat
     1981 gtttgaactc ctggttccat gcgaatcaaa taggacacat cggaatgaat gcgctgattg
     2041 agttctacca gaaagtcgat tgtcttttgc tttctacgat tggactccac atagtacttc
     2101 agcagcggcc cgttttctct cagagcaagg taaggcgcga gctgtgcttt tacgtctgga
     2161 gaatactgga agggaaactg ctccgcatct ggctcaagaa agaaatcaaa cggattgatg
     2221 gagtgaagtt ctgcgaccag atccaccgtg aaatccaggc gatccgtttt ctccggaaac
     2281 acaagcctgg ccaggtggtt tccaaaggga tcttgctgcc agttgatgaa atgaggttcc
     2341 ggcccgacgc gaaaggaata ggaaagaatg ggagtgcgtg aatggactgc gggcttgaga
     2401 cgcactacgt gaggaaatgc agtgactgca tgatcgtatc gatacgaagt cttatgatgt
     2461 aatgctacgc gaattgccat atatctgagc aagcacatcc gtgctcgcaa cagtagtgca
     2521 aagagcgtac cccgcctaac accttgcaga ggtcccgacc tggtcagaaa gcgaggatcc
     2581 gccaggtctg aacgattgcc tgccagaacg tccaatatgg cgttggtctg ggtttagaaa
     2641 ccgccatgcc gcaattccgt cggtccagcc cgtccgaaag caaattcgag agactaccgg
     2701 gaattcccga aacaaaacac gcaccttaac acgcatacta taaaaacgcg tattccgata
     2761 catgaattat atttcttatt ttttgattgc gtcatttatg ttttgtgttc cattaaaaat
     2821 ggaactgatc cacgctctgg caaccagcct gctctcacct atggttcgct gctctgtgtt
     2881 cgaagtcggt cttgtgaccg gacgcagaat gcaggatctg aaaaaagtcg ggtggttctt
     2941 gatcgccttt gcggtcgtta tgccgttgat tcatggattg ccgggtgtct tgattggaac
     3001 atggaccggt ctttcgatcg gaggaacttg cgttgccaga atctcccatc acctaccacc
     3061 tgacagcttc tattgtagtc acttttcctt ttgacgttgt ttgtggaatt cctttgtatt
     3121 acactctagc gggagccatc caatgaatct acaccacgta aaactgctga ccatcattgc
     3181 cccggacaat ctagaagcca gattgaccag tgacgttcgt ctcttcggag cacgcggata
     3241 tacgatttct cccgcggtag gagcaggact cagcagcgtg cgcgacaacg aatggactgg
     3301 aaaaaatatt cggcttgaga cgcttgtagg gcaagacgtt gcatacgctt tgatggcgca
     3361 cctggccaaa gattattttg aaaagttcgg agtgattgcg ttcatgtccg atgtagaggt
     3421 gcttcgcaaa gagcgatttg actaccgcta acgatccagc tctcgcgttc gattgagaat
     3481 ggaatcaatc tcaggcgatc ttgcatgtgc aactggtcgt ggtttatttc tgcgagacca
     3541 gacccacaaa agcgcaaatg ctcccacaat cgctaccaca atccagggcc acaccacggg
     3601 cggctttacg aagatcattt ttccaaaacc ttttacaaaa ccgctgacca gatcgactct
     3661 gccttctgcc tgcagaatga ttacttgagg atctttacga atttcttccc cgaaccctga
     3721 gacaagtcct tcttctatgc tgctttcgct tctgccttca atgatgcggt tttcaatgaa
     3781 ccgacgaagc tggactccga aacaattttc ttcgatgtgt ccgcatgttc ctatgatact
     3841 gtggcaacca cagtaacaat tcaccttcga cgtaagtcga ttgaacatcg caatctgagc
     3901 agaatcagta aggtgttcgg gagcacggcc ctgagcctgt gccgagctgg aaatcatcag
     3961 agccagaaac gcaatgccca cgcttctgac agaaaaaaga gccgagaaac tcatattcat
     4021 caaggtttca gaaactaccg tcgaaacaac caaaaataga tcgctgttag gcgcccgggt
     4081 atgtttgaaa ttgacgcaaa tctgactcgg ctaaaatgac aaaaatgggc ctgacggtcg
     4141 ccatcgttct tggactgctt cttgcggtct gcgcagcatg gattgcgcgc gagttacgca
     4201 atagacgcaa ccagatcgct cgtcagttgt tcctgagaca cggctccgtg atgcttctga
     4261 tcaaacctga cgacgggagg attgcgtttg cgaacctggc ggcggcccgc ttctacggct
     4321 attcaccgcg tcagctcgag tccatgtccg tcaaagacct gaacacgctg ggtccagcag
     4381 aagtccaggc tgaactggat cgcgcgcgaa gggaagagcg cagctacttc cttttccggc
     4441 atcgagttgc aagcggtgac attcgcacgg ttcaagttca ttcttctccg ctggtcatcg
     4501 atggagaaac ctttttgctc tccatcattg atgacgtgac ccaccacgcg aagctaacaa
     4561 aagagcgcga aaccagaggg cgcgttcttg aactgctcgc tgcaaacgct cccctggaat
     4621 ccatcctcac tgaacttgta ctggcttacg aagatctcgc gcccggttcc atttgctcta
     4681 ttcttctcac ggacagtgag gagagcacac ttcttcctgg tgccgctcct ggtcttcccg
     4741 aatggttcaa tcatgaagtg cagggactct ccacaggaga aggccaggga tcgtgcggaa
     4801 cggccatcgc gcgaaagcaa agagtcattg tttcaaacat cgcaacggat cctttgtggg
     4861 ataaatatcg ccatctcgca gaacgcgcag gccttgccgc atgctggtcc gaaccgattt
     4921 tttcaggatc aagaaaaatc ctgggatcat ttgcaattta ctacaaacag gttcgcgagc
     4981 cacacgaaga agatcttcaa ctcatctcca ggcacgcaca actggccagt ctctgcattg
     5041 aacaaacgca actccgcagg cgccagcaac tggccgatca cgtatttcaa aatacgggag
     5101 aaggaattct catcacggac gacaaaagtc agatccttgc ggcaaaccct gccttcggca
     5161 aactcactgg ctatgaacct gctgaaatcc ttggcaaaac acccagaatt ctttcctccg
     5221 gaaaacaccc gaaatcgttc tacgaagaca tgtggggaca gatcaggaac acaggcaaat
     5281 ggaagggaga aatctggaac aaacgtaaga atggagaact ttatcctgag ctgctgagca
     5341 tcagcacggt atacaccaaa ggatctgtca ttggatacta cgtggctata tttactgata
     5401 tctccaacat caaagaaaca gaggctcgcc ttgacgagct ggcgtaccgc gatccaatca
     5461 cagcgcttgc caatcgctcc ctgtttcaat tgcgactcgc agaagcattg catcgcgcag
     5521 aaagatcagg cacttctgtg tggatattgc tcattgacct ggatcgattt aaggatgtga
     5581 atgatgcgct cggtcacgaa gttggaaaca aactgcttga ggctgtaaca ggaagatttt
     5641 tagaagtgct cgaacccggg gacttgcttg cgcgcatggg aggagacgag ttcggcttgc
     5701 tccttgaaaa cgcagacgct ggaagaatcg cgcaacggct gctccattcg ctaaacgcac
     5761 ccgtccatct caaaagcgga gaccaaatcg atgtgggtgc aagtgtgggg atcagtcagt
     5821 ttccgcatga cggtaactct gttgaacagc tcatacagaa tgcggatgct gccctctatg
     5881 atgcaaaggc gtccggaaga agaacattcc gattttacac cagcacactc actgcccgcg
     5941 cgaaagccag actctctctc gaaacaaaac tgcgtcgtgc gctggaaaga aacgaaatcc
     6001 ttcttcacta ccagccgcag gtgaacattc gcacaggtaa aattgtagga gtagaagcac
     6061 tcgctcgatg gccggacggt gatcaaatga ttccaccgga tcaatttgtt ccggttgcag
     6121 aagaatcggg gctgattctc ccgctgggtc aatctctctt ccgaaaagca tgcatgcaag
     6181 cacgacaatg gaatgacgca ggcatctctc tcatggtggc tgtgaacctt tctgccaggc
     6241 aatgtgcaaa cgctcgtctc gcgcacgaaa tcgcggaagt tattcgcgag acaggagtga
     6301 atccagaaca actcgaactt gaaattactg aaactgctct gatgagtcag gggcaagatg
     6361 cgctcaatct gctggaacaa atgagaaaga ttggagtgcg catggctcta gacgattttg
     6421 gaacaggcta ttcttctctg gcctacctcc gtcactttcc agtcgatact ttgaagattg
     6481 accgcagttt tatcagcgaa atccccaccc acaacagtgg tgctgaaatc acatccgcca
     6541 tcattcgcct cgggcattcg ttgaacttca cagtgctcgc agaaggcgta gaaacagaag
     6601 cacagctcgc gttccttgca aaacaagaat gcgacctcta ccagggctac taccgcggac
     6661 gagccgtccc gccggaagaa atcccggctc ttctgcaata aacccatcga atacttagtg
     6721 cttgacaaac ctaacaccgt tagttttatt gtccccagac agctaacact gttaggagta
     6781 attatggaac cgagactcac tttaccggcc gttcgggata cgatctcgaa agaatctttg
     6841 cgtaatccca catggaaagg attgctatac tttgcacgag acatcgcagt ctacgctgcc
     6901 attctggttg gactctattc gacagacgcc tggtatttcc tggtgcctct gtggattctg
     6961 gcaggcctgg ccgtgagcgg ccttttcatc atcggtcatg acgccgcgca cggggcactt
     7021 ttcaaaaatc agaagctggc gtatttgatc ggacaaatat ccatgctacc ttccctgcac
     7081 gcgtatcacc agtgggtata cggacacaat cggattcacc atggccatac catcaagatg
     7141 gaaggcgact ttgtctggcg cccaacttcc cccgctcagt atcgcgacct ttcctgggca
     7201 ggaaagattt ggcacagaat cacatggtct gcgatcggct ctggcatcta ctatctagtt
     7261 caaatctggt ttggaggaat gatcctgttc gtcgcgcaga cgaaaggcgc aaagcgagat
     7321 agaattctga tcattctggc agcaatcgcg atgattgccg ttccaatcgc actgtttcca
     7381 acctggcagg caggcctctg ggtggccgtg aagctgggcg tcattccatt tcttgcattc
     7441 aactactgca ttggattctc tgtctatgtt catcatatcc atgacttcat tccatggaag
     7501 aagcacaaag actggacgcc gttctacgga cagatgcttg gaactgtgaa ctaccacatt
     7561 cctgctttct tcaatttctt cttacacaac attttcctgc acatgcctca ccacgtgaat
     7621 atgcgcgtgc ctttctacaa ccttggacgg gccttgagcg agatcaaatc ggtgtatgga
     7681 caatatgtga ttgaaagaaa atcgatgttc atggactatc tacacactac cagaaagtgc
     7741 aaactgttcg acccagaaag cggaaagtgg ctaacgtacg cggaagcggc ggaagaaaga
     7801 acgccaaacc tcagccagtc cgtgccggcc tgagccggga atctatcctg gatgccgccc
     7861 tcgaaattct aaacgagggc ggtatggccg ctctcagcct tcgcagcatc gtcaagagac
     7921 tgggctgcag cgtcgcagga ccttacacat atttcgcggg acaggatgaa atcatcagca
     7981 ctctcatcca gagaggagaa gaggaactca caggagacct caaacgcgca ggccaggaag
     8041 gcggagatct tttcgaagtc ctaagaagac tggccgccac atacatgaca ttcgcgcggc
     8101 gcaaccgcga gctgcacaaa ctcatgttta acatcaatgg acacaaacgc atttttcctt
     8161 ccgcaaccgc aagctatcgc gcctatattg agaccatccg gggtggagta aaaagtggca
     8221 gacttcattt ttcacgcaaa ggctaccacg ccatagcgcg aactatgtgg gcctggctct
     8281 acggattgat tgttctagaa atgaccggcg ttaccacgat gaatgcagat gaagcaatcg
     8341 acgaaggatt cagctttttc cagcgcttgc tggagcaggg agagaaaagc gcctaacggg
     8401 gctgcggatt cactacaacc agtgcctttg tttctcgact caaaaactga tctaaatctg
     8461 tcttgctcaa tccgaattcg tagaaagcat gtgacgcctg atccgcgtac cagaagtagt
     8521 tcatgggcct gcccagttcc acttccgggt attcgagagt cttaaagaaa tatttagaat
     8581 ccaccagata gaaccatttc tcaaatcgtc tcaagaatcg attcatgcgc gaattgcgaa
     8641 actcattgtg atctaacctt tcgtcatgcg gaaaatccag tcttcgcacg cgacgagcca
     8701 gatcgtgaaa acgcgtaact accttatcca tgcgaaatgg tttgtgttcc agtctgaatt
     8761 caaaaagagc aatggaaatc aaatgagtgg ccagcagtgg aagacccttc tgcaaggaca
     8821 tgcgaagcag gtggttgaga tgaggctttt ctgcagtgtt tcccggcgga tgaatgggag
     8881 acccgggatg aacccacaga tcacttcttc ccagaagatc ggggcaaaga aaatctcctg
     8941 ttacactcag aggaacaagt ttcaagctat agtcccgcgc aacgttatcc ggcatagagg
     9001 gctcgcgaat tctgccccga agatgtggtt cctggtctgc gcaaagaatg gtctctgcca
     9061 gatttgaatc gtcaggaata tcgagagcat catctgccgc aggatcttct gccgtatcag
     9121 gcgcttcggg aaagaatcga ctcaggcttg agaaaaggga agaagcctct cccagacgag
     9181 agacaactcc agaaacttta tgtggagagg cgaatggttc ccggggccaa ttttttgcaa
     9241 cgaagctccc gcgacgcata ggacgattga gcaaaaaatg aatgagagca agctcatcga
     9301 gcgcggactt gcggctccag acgtagcagg tttttagtgg atctgtatcc ggcgcgcgac
     9361 caaggaaaac acgttctatc ccaccgggcc atctggccat ccgcagaagc cagcgcatgg
     9421 cgc
//
LOCUS       DAORFF010000074         9364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3462_length_9364_cov_3.826834, whole genome shotgun sequence.
ACCESSION   DAORFF010000074 DAORFF010000000
VERSION     DAORFF010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9364
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3462_length_9364_cov_3.826834"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PKK76_04655"
     CDS             <1..946
                     /locus_tag="PKK76_04655"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL67788.1"
                     /translation="LGWMVVFNVAFGIYVYRRIFSRVDKLKLASEKMATGSLDARVNW
                     KWRKDELDLLGRSFNAMAETIETQMKQISRLNLEMNEEMRIGREVQQVFLMQQEEITS
                     RGIAMYFQPLRQVSGDVYFFQTINYKDRTYEVFFLADATGHGVPAALITSILLLSLRE
                     ALASTLHPGKVLMKLNDLMYERLQSQYYATGCIFLRDDQGRLHYASGGHPSPLWFRPS
                     TGQILELEATGPMLGMIDGVDFEGRSMPVQKGDRILIYSDGVTEGARDHEMFGLDRLK
                     QVVLASNNPDHLVQSVWENLNSFVDHHVDDVTIFSFEI"
     gene            949..2355
                     /locus_tag="PKK76_04660"
     CDS             949..2355
                     /locus_tag="PKK76_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL67789.1"
                     /translation="MRRWIPAFVLILLLILSFGASRSILKARLLELRIAIQRDQILRY
                     ELSSRLLRYRFQQMLEDRDNIRSEIKLTVLESHVVNDLSQEASEPGWMERGGAILING
                     VRLLSAKPRLDILEKRIKVLQLHAGFYHERNRNCPGALARYQEFELSEDSPDTAFVML
                     HTGYCQAVTGKIPDAIATLERVEGKFPGTHFERSAKEILLLLRGMQKKAESIRGKSDE
                     EAASTLFESGQFWQVDQLLDKKIVSGRQKYMLARSKEEIGQTAAAVRIYRELTSESDP
                     ELSKLANRRMLLMGTVYNQGESIAKEARQNAQRTGDTAIVQEINQAVVAQKPAELLKV
                     SAKEAPEVKFVSEIKTEIEKREALIEATPAREEPPPGISVVLSDGRTLQGFSILMEDD
                     RAILAGSRPVAVPAILIASIRLAPSRTDYNVCAIDRSRHVLCGEAIEGKNQEFAVGGD
                     SMPLTSIEAILVQKRGVQ"
     gene            2352..2708
                     /locus_tag="PKK76_04665"
     CDS             2352..2708
                     /locus_tag="PKK76_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL67790.1"
                     /translation="MSNSAELEISEILPFEKGFRVNISGPINIYSAGQLTNAIIKKTT
                     DGMKLAVDLSKVPMVDSSGIGFLIRLARDLEKKKGKVILCGVTDDFKSNLTALRVDKL
                     VRLASSAATIPADFWD"
     gene            complement(2745..3797)
                     /locus_tag="PKK76_04670"
     CDS             complement(2745..3797)
                     /locus_tag="PKK76_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67791.1"
                     /translation="MLASASTVSQNPVKSVYSFVTNVATNPTLMTVPAVDTTRAFVMC
                     SYRLANTSFQTNRVPICSLTSSTQVTVQAGTSDAGVLVRGYVVEFSTGVNVQRGAFTL
                     AGGTLNNTVSISPVSQNRSFVIVQSRTTDTGTLIDEHRTVMGYLQSSSSLYVVRNQAT
                     IITDVEYQVIEMQGATVQYGISQIPGGSFSVSIPIAAVNTSKSFVVFSSAADNLSNGN
                     DSEMAVRCDLANASQLLCQRQDSTNAPDIAWYVVSFADSTYVERGSADTNVFTPPANN
                     TDTSMTVTPSVPFDLTRTLPIGSTASDPGDADAGLDSASFTQHLLTSTQLNIQRGSAD
                     DRRSVASWQLIQFPAR"
     gene            complement(3905..9334)
                     /locus_tag="PKK76_04675"
     CDS             complement(3905..9334)
                     /locus_tag="PKK76_04675"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNL67792.1"
                     /translation="MVPFGFLKQPVKPFDTNCAELGSERWADEKNWLFDFKEDLSAGI
                     KCRFTMKSGLTTISGKEVGGQKVFEFSTGGPAVVNSRPWNGAYIAEDQIFVLRLDTEV
                     DESSVISGLGFQVAGLREKVGARIVTGSERQAILKTQYNQDEPQRIVLVQSKQTFPYG
                     AKVKLIWGRSITSKSGVAGDQDQILEYTVRNDFTASFSCERENAQADCIPMLPAYISF
                     SSPISKEQALRVVIRGAGKTFSPKLSEDDEDPGISGVSVPGPFPENTDFRVELPGDLR
                     DDANRPLSNANRFPLSFRTASYPPLAKFAAPFGILELKEGGLLPVTLRNIEAPVPVKY
                     HEAGEGMPGRKIKITPENADQVLYWMRRVSSHNREKSIFQGQDVKISDLRLPRPNGDK
                     AFEVIGIPLGGPGFYVIELESRILGNSLLGQDKPLYVPAAALVTNLSVHFKWGRESSL
                     VWVTTLDAGKPVAKAEVAVRDCNGKVWFTGSTDSEGTVRTQSIPENAPSCSKRPFSSG
                     LLVTAVSGGDMGLVHTDWNDGIETWRFNLPYGGSHKPLLGHTIFARTLLRAGDTLNMK
                     HLIRRHQMSGFGDVAPGLLPSIAVVTHAGSGQEFTFPLQWERGASETTWRIPTDAKLG
                     EYTVAFKGGSLEYAMDTGSFQVEEFRVPLMRGIIKPPAEPVVRSSGFPVDIALSYLSG
                     GGASNASVRVRYAIRASGNPEFRDLDDYVMANGSVKEGTVAQGDENSEEPSESPSQAK
                     VKSQDITLDRSGTSRVRITDLPSAETMQSVETEMEFRDPNGVVQTVSRTIPVYPSVHF
                     IGIKQDAWMVSKDNLKFQVIVVDLKGNPVAGTNIRVDAYERKNFSHRKRLVGGYYAYD
                     SSTETKKFGSLCEGKTDYRGIVLCQTKVDRSGNILLGAFAKDSQGNTIAANREIWVAG
                     SDAWWFRPDEGDRMDLLAEKKRYEPGEIAKLQVRMPFSKATALVTVEREGIVEKFVRE
                     LSGKEPVIEIPVKGNYAPNIFVSVMAVRGRSGETRPTALVDLGKPSYRLGIAQIVVGQ
                     KAHELSVKVEPENDVYPIRGKARVKVTVRGPNGMSLPPNSEVALAAVDEGLLELKPNT
                     SWNVLPSMMGSRGLEVRSSTAQMQVVGRRHFGLKALPPGGGGGQQMTRELFDTLLLWK
                     GRVKLDSQGEATVEIPLNDSLTAFKIAAVATGGAGFFGTGTASIRTTQDLMLLPGIPP
                     LVREGDKFRSEFTLRNTTNRSMSVELSARISASAGEGPVVPAQKIDLSAGEAREVGWD
                     INVPAGISSLTYDVQAVEAGGNARERVKIVQKVIEAIPVRTFQATLEQIDKEYVVPVE
                     KPKDAIAGRGDLRVNLSPSLTGSLEGVREYMKKYPYTCMEQRVSRSISLRDTKMWAET
                     MERLPLYLDSEGFVKFFPEMRFGSELLTAYILAISAESGYEIPAGPREKMIQALQAFM
                     DGKISRQYPLNTTDLSLRKLTIIEALSRYGESKPQMLETIQIQPNLWPTSAVIDWYNI
                     LERTPSIVGRTDRMKEAQQILRSRLSFQGTTMSFSTEGNDYMWWLLTSPDMNAIRMTL
                     AVMKNPDWRADLPRIMRGTIGRQRKGSWDLTLANAWGVLAVEKFASEFERITVGGTTS
                     ASLGDQTRKLDWSKARGGDLILPWPANRTNMSVRHDGAGKPWALLQSRAAIPLKEDLH
                     AGFRIKKSWKMIEQKKSGIYSRGDIVRVTLKITADADRTWVVVSDPVPAGATILGTGL
                     GTESSLMQQGEKREGWAWSAYEERSFEAFRSYYEYAWKGEWTVEYTMRLNQSGTFQLP
                     QTRVEAMYSPEMFAESPNEVLSVMP"
BASE COUNT         2321 a         2488 c         2284 g         2271 t
ORIGIN      
        1 cctgggctgg atggttgtat ttaacgtcgc atttggaatc tatgtgtatc gcaggatctt
       61 ctcacgcgtg gacaaactga aactggcgag cgagaaaatg gctaccggaa gtctggatgc
      121 acgggtgaac tggaaatgga gaaaggatga actggatctc ctcggtcgtt cgtttaacgc
      181 aatggcagag acgatcgaaa cacagatgaa acaaatatca cgcctcaatc tcgaaatgaa
      241 cgaagagatg cgaattggac gcgaagtgca gcaggtcttc ttgatgcagc aggaagaaat
      301 aacgtcacgc ggcattgcaa tgtattttca accactcaga caggtcagcg gggatgtata
      361 tttctttcaa acaatcaact acaaggatcg cacatacgag gttttctttc tggcggatgc
      421 aaccgggcac ggcgtgcccg cggcactcat cacttccatt ctgctactga gtttgcgcga
      481 agctctggca tccacgctcc atcctggcaa agttttgatg aagctcaatg atctcatgta
      541 tgagcgtctg caaagtcagt attacgcaac aggttgtatt ttcctgcgag atgatcaagg
      601 tcgccttcac tatgcaagtg gtgggcatcc atctccgctc tggtttcgcc cttctacggg
      661 gcaaatcctg gagcttgagg caaccgggcc catgcttggg atgattgacg gcgtagactt
      721 cgaaggccgc tccatgccgg tgcagaaggg cgatcgcatt ctgatctatt ccgatggtgt
      781 aacagaggga gcccgagacc atgagatgtt tggactggat cgtcttaaac aagttgtttt
      841 ggccagcaac aacccggatc acctcgttca atccgtctgg gaaaatttga actcattcgt
      901 tgaccatcat gtagatgacg tcaccatttt ctcgtttgaa atttgaatat gagacgctgg
      961 attcctgcat tcgtcttgat tcttctcctg atcctgtctt tcggagcttc gagaagcatt
     1021 ttgaaagcac ggttgcttga gttgcgcatc gccatccaga gagaccagat tcttcgttat
     1081 gagctttcat ccagactgct cagatatcga ttccagcaaa tgctagaaga ccgagacaac
     1141 attcgcagcg aaatcaaact cactgtgctt gagtcgcacg tggtgaatga tctttcccag
     1201 gaagcctccg aaccgggctg gatggaacgc ggtggagcaa ttctcatcaa tggtgttcga
     1261 ctgctttccg cgaaacccag actggacatt cttgaaaaaa gaattaaagt tctgcaacta
     1321 catgctggct tctatcacga aaggaatcgc aattgtccag gtgcgctcgc gcgctatcag
     1381 gaatttgaac tcagcgaaga ttcgccggac actgctttcg taatgctgca caccggatat
     1441 tgccaggcag tgaccggaaa gatccccgat gcgatcgcga ctctagagcg agttgaagga
     1501 aaattccccg gcacccactt tgagcgaagc gcgaaggaga ttctactctt gcttcgcggc
     1561 atgcaaaaga aagctgagtc gattcgcggc aaatcagacg aagaagcagc aagcactctc
     1621 ttcgaatcag gacagttctg gcaagtggat caactcctgg acaagaagat cgtgagcgga
     1681 cgccagaagt acatgctcgc cagaagcaaa gaagaaatcg gccaaacggc cgccgccgtt
     1741 cgcatctatc gcgaattgac atcagaaagc gatccagagt tgtccaaact cgccaatcga
     1801 cgaatgcttc tcatggggac tgtgtacaac cagggcgaaa gcatcgcgaa agaagctcgc
     1861 cagaacgcgc agcgcacggg agacaccgcc atcgttcaag aaatcaacca ggcggtagtc
     1921 gcgcagaaac cggcagagtt gctcaaagtt tcagcgaagg aggctcccga agtgaaattt
     1981 gtatccgaga tcaagactga gattgaaaag cgcgaagctt tgatagaagc gacccctgcg
     2041 cgcgaagagc cccctccggg aatcagcgtc gtgttgtcag atgggcgaac ccttcagggt
     2101 ttctctattc tcatggaaga cgatcgcgcg attctcgcag gttcgcgtcc cgtcgcggtc
     2161 ccggcgatcc tcatcgcgtc aattcgactt gcgccttcgc gtaccgatta caacgtgtgc
     2221 gctattgatc gatcacggca cgtactctgt ggcgaagcga tcgaaggcaa gaaccaggaa
     2281 ttcgcggttg gaggagacag catgccgctg acgtccatcg aagcgatcct ggtgcagaaa
     2341 aggggggttc aatgagcaat tctgcagaac ttgagatttc agaaattctt ccttttgaaa
     2401 aaggatttcg agtaaatatt tccgggccca tcaacatcta ttcggccggg caacttacaa
     2461 atgcgatcat aaagaaaaca acggatggga tgaaactcgc cgtggattta agcaaagtgc
     2521 ctatggtgga ttcttcaggc attggttttt taattcgcct cgcacgtgac ttggaaaaga
     2581 agaaaggaaa agtcattctg tgcggagtga cggacgattt taaaagcaac ctgacggcac
     2641 tgcgagtgga caaactcgtt cgcctggctt cctcagccgc gaccattcct gctgacttct
     2701 gggactagcc catacgcgta gcacctaaca atgagaaggt gatgctaacg cgcaggaaac
     2761 tggatcagtt gccaggatgc aacgctacgc cgatcgtctg ccgatcctct ttgaatgttt
     2821 agctgcgttg aagtaagtag atgctgagtg aatgacgcgg aatccagacc cgcgtccgca
     2881 tctcctggat ctgaagcggt ggatccaatc ggcaacgtcc tggtcagatc gaatgggaca
     2941 gagggagtca cagtcatgct cgtgtccgtg ttgtttgccg gcggagtgaa tacattcgta
     3001 tcagcgcttc ctcgttcgac atatgtgctg tccgcaaaag aaacaacata ccaggcgata
     3061 tctggggcat tcgtggaatc ctgtctctga catagcaact gagaagcatt ggcaaggtca
     3121 cacctcactg ccatctcact gtcatttcca ttcgaaaggt tgtctgccgc agaactaaaa
     3181 acaacgaaag acttcgaggt attcaccgca gcgatcggaa tcgacacact gaacgatcct
     3241 cctggtatct ggctgatgcc atactgcacc gtcgcgcctt gcatttcgat cacctgatat
     3301 tcaacgtccg taatgatggt tgcttgatta cgaaccacgt agagactact tgaactctgt
     3361 agatacccca ttacggtccg gtgttcatca atgagtgttc cagtgtccgt ggtccttgac
     3421 tgcacaatca caaatgaacg attctgactt acgggagaaa ttgagacagt gttgttcagt
     3481 gtgcctccgg ccagagtgaa agcaccgcgc tgtacattca cgccggtaga aaactcaacc
     3541 acgtaacctc gaaccagaac cccggcatct gaggtccctg cctgaacggt tacctgcgtc
     3601 gaggaggtga gagaacaaat cggaacccgg ttcgtttgaa agctcgtatt tgcaaggcga
     3661 taagaacaca ttacaaaagc tcttgtcgta tctacggccg gtacggtcat cagagtggga
     3721 ttggttgcta cgttcgttac gaaggaatac accgacttaa ctggattttg cgaaaccgta
     3781 gacgctgaag cgagcaacag cgccaggtaa ttcggctggg gataacgcga acattgcgaa
     3841 accccaacta taaccaataa gacgaactta cgcaacatcc aaccatcccc tggttctaga
     3901 tcgctcaagg cataacgctt aacacttcgt taggcgattc tgcaaacatt tccggagaat
     3961 acatggcctc cacacgcgtc tgcggaagct gaaaagtacc ggattgattc aggcgcatgg
     4021 tgtattcgac agtccattcg cctttccacg cgtattcgta ataagaacgg aatgcctcaa
     4081 aggacctttc ttcgtatgca gaccacgccc atccttcccg cttttcgcct tgctgcatca
     4141 agctcgattc cgtaccaagt cccgtgccaa gaatcgtagc gccggcagga acaggatcgg
     4201 aaacaaccac ccacgttcga tccgcatctg cagtgatctt cagtgtgacg cgcacgatat
     4261 cgccacggct atagattcct gatttctttt gctcaatcat cttccaggat ttcttgatcc
     4321 gaaagccagc gtgcagatct tctttcaatg gaatggcagc tctactctgc agtaacgccc
     4381 atggcttccc tgccccgtca tgtcgcacgc tcatgttggt tcgattcgca ggccagggaa
     4441 gaatcaggtc tccgccacga gccttgctcc agtccagttt tctggtctga tccccaagag
     4501 aagcggaagt ggtcccacct accgttatac gctcaaactc agaggcaaat ttttcaacag
     4561 ccagaacacc ccaggcattc gccagagtta gatcccagga accttttctc tggcgaccaa
     4621 tcgtacctcg catgattctg ggaagatccg cgcgccagtc cggattcttc atcactgcaa
     4681 gagtcattcg aatcgcgttc atatccggtg atgtcaaaag ccaccacatg taatcgtttc
     4741 cttccgtaga aaaggacatg gtggttccct ggaagctcag acgactccga agaatttgct
     4801 gcgcctcctt cattctatct gttcgaccaa cgatagacgg cgttctctcc agaatattgt
     4861 accaatcaat gactgcagaa gtcggccata gattcggctg aatctgaatg gtctcaagca
     4921 tctgcggctt tgattctccg taacgcgaaa gtgcttcgat gatggttagc ttgcgaagac
     4981 tcaggtctgt tgtattcaaa gggtattgtc tggaaatctt tccatccatg aacgcttgaa
     5041 gagcctgaat cattttctct ctgggcccgg ctggaatttc ataaccagac tcagcggaaa
     5101 tggcaaggat ataggcagtc aaaagctcgc tgccaaatcg catttcagga aagaacttaa
     5161 caaatccctc cgaatccagg tacagcggca atcgctccat cgtctctgcc cacatcttcg
     5221 tatcgcgcaa actgatagaa cgagagactc gttgttccat gcaagtatac ggatatttct
     5281 tcatgtattc gcgcacacct tccaggcttc cggtgagaga aggactcagg tttacgcgaa
     5341 gatctcctct gccagcgatt gcatcctttg gcttttcaac tggaacaacg tactctttgt
     5401 cgatctgttc cagagtggcc tggaatgtcc gaacaggaat cgcctcaatt actttctgaa
     5461 caatcttcac tcgttcgcgg gcattgcctc cagcttccac tgcctgcaca tcatacgtta
     5521 gacttgagat tcctgccggg acgttgatgt cccagcccac ttcccgcgcc tctccggcgc
     5581 tcaggtcaat cttttgcgcc ggcactaccg ggccttctcc ggcagaggcg gaaatcctgg
     5641 cgctcagctc aacgctcata gatcgattag ttgtatttct taaagtaaat tcggaacgaa
     5701 atttgtcccc ttctctgacc agaggtggaa ttcccggcag aagcatcaaa tcctgcgttg
     5761 tgcgaatcga tgcagttcct gttccaaaga acccggcgcc tccggtggca accgccgcga
     5821 tcttgaaagc ggtcaacgaa tcattgagcg gaatttctac agttgcttct ccctgtgagt
     5881 caagcttcac gcgccctttc cacaaaagca aagtatcaaa gagttcgcgc gtcatctgtt
     5941 gtccgcctcc gccgccagga ggaagcgcct tgagaccgaa atgacgcctg cccacaactt
     6001 gcatctgggc ggtagagctt cgcacttcca ggccgcgaga acccatcatg gaaggaagaa
     6061 cattccagct ggtgttgggc ttgagctcca ggagaccttc gtccaccgct gcgagcgcaa
     6121 cttctgaatt cggtggaaga gacattccgt ttggtccgcg aacggttacc ttgactcttg
     6181 cttttcctcg aatcggatac acgtcgtttt caggttctac ttttaccgaa agttcgtgag
     6241 ctttctgccc aaccacaatc tgcgcgattc caagacgata cgatggtttt cccagatcca
     6301 cgagtgcagt cggtctagtc tctcctgaac gccctcgcac tgccatcacc gaaacgaata
     6361 tgttaggcgc gtagttgccc tttactggaa tttcaatgac aggttctttt ccactgagtt
     6421 cgcgaacaaa tttttcaacg atcccttcgc gctctacagt tacaagagca gtcgctttgc
     6481 taaacggcat tctgacctgc agctttgcga tttctcctgg ctcgtatctc ttcttttcag
     6541 cgagcaaatc catgcgatcg ccttcatccg gacgaaacca ccacgcgtca gaaccagcga
     6601 cccagatttc acgattcgca gcaatcgtat tgccctgaga atctttcgcg aaagcaccaa
     6661 gcaagatatt tccggagcga tctacttttg tctgacacaa tacaattccg cgatagtccg
     6721 ttttgccttc gcacagactt ccaaacttct tagtttcagt agaactgtcg tatgcgtagt
     6781 aaccgcccac cagacgcttg cgatgcgaaa agttttttct ttcatatgca tccactcgaa
     6841 tattagtgcc tgcgactgga ttgcctttca aatccacaac aatgacctgg aatttcaaat
     6901 tgtcttttga aaccatccag gcatcttgct tgattccaat gaagtgaacg gaaggataca
     6961 caggaatcgt gcgcgaaaca gtctgcacca ctccgttagg atcgcggaat tccatttcag
     7021 tctccacgct ctgcatggtc tccgcagaag gaagatctgt gattcgaacg cgagacgtcc
     7081 cggaccgatc gagagtgatg tcctgactct tcacctttgc ctgcgaaggt gattcggacg
     7141 gctcttcgct gttttcgtct ccttgcgcca ctgtgccttc tttcacggaa ccgtttgcca
     7201 tgacgtaatc gtccaggtcc cgaaactcag gatttcccga agcacggatc gcgtagcgca
     7261 cgcgaacgct ggcattggag gctcctccgc cggacaaata agaaagtgct atatccaccg
     7321 gaaatccgga cgatcggacc acaggttcag cgggtggctt gataattccg cgcatcagtg
     7381 ggacgcgaaa ttcctctacc tggaagctcc ctgtatccat cgcgtattca agagatccgc
     7441 ccttaaaggc gacagtatat tcgccgagct tcgcatcggt tgggattctc cacgtggttt
     7501 ctgatgcgcc gcgttcccat tgcagaggga aagtgaattc ctggcctgat cctgcatgag
     7561 tcacaactgc aatggatgga aggagaccag gagcaacgtc gccaaagcca gacatctgat
     7621 gacgacgaat caaatgtttc atgttgagag tatcgcctgc gcgaagtaag gtgcgcgcga
     7681 agattgtgtg accgagcagt ggtttgtgac ttcctccata cggaagattg aatctccacg
     7741 tttcgattcc atcattccag tccgtatgaa ccaggcccat atccccgcca gagactgcag
     7801 tgaccaggag cccgcttgaa aaaggacgct tgctacaact cggcgcgttc tcggggatgc
     7861 tctgtgttcg cacagttcct tctgaatcag tggaacccgt gaaccatact ttgccattgc
     7921 aatcgcgcac ggccacttcc gctttcgcca caggctttcc tgcatccaga gttgtgaccc
     7981 atacgagaga ggattcgcgt ccccatttaa agtggacgga aagattggtt acgagggccg
     8041 ctgcaggaac gtacagtggt ttgtcctggc ccagaagaga atttccaaga atgcgacttt
     8101 ccagttcgat cacataaaag ccaggaccgc ccagcggaat tccaatgact tcaaatgcct
     8161 tgtctccgtt gggtctggga aggcgcagat cagaaatctt tacgtcctgc ccctggaaga
     8221 tcgacttctc tctgttatgc gaagaaactc ttctcatcca gtagagcact tgatccgcat
     8281 tctccggagt gatcttgatt tttctgcccg gcatcccttc tccggcctca tgatatttta
     8341 caggaacagg cgcttcaatg ttgcgaagag tgacgggcaa cagaccgcct tccttgagtt
     8401 ctagaattcc aaatggtgcc gcgaacttgg cgagaggtgg atacgatgcc gtgcgaaagg
     8461 aaagaggaaa gcgattcgca tttgaaagcg gccgattcgc atcgtcgcga agatctcccg
     8521 gaagctctac gcgaaagtca gtattctcag ggaagggccc aggaacggat acaccgctga
     8581 ttcctggatc ctcatcgtct tcgcttaact ttggagaaaa agttttgccg gctccgcgaa
     8641 tgacaacacg caacgcctgc tcttttgaaa tgggcgaaga aaaggaaatg taggcaggaa
     8701 gcatggggat gcaatctgcc tgagcgttct ctctctcaca gctgaacgaa gcggtaaaat
     8761 cattgcgcac agtgtattca agaatctgat cctgatctcc tgcgacgccg gactttgatg
     8821 taattgatct accccaaata agttttactt tcgcaccgta ggggaacgtc tgtttcgatt
     8881 gaacgagcac aatcctctgt ggctcgtcct gattgtactg agttttgagg attgcctgtc
     8941 tttcagagcc agtgacgatt cgcgctccaa ccttctctct cagacctgct acctggaaac
     9001 ccaggccaga aattacgctc gattcatcta cttccgtatc caggcgaaga acaaaaatct
     9061 gatcctctgc aatgtacgca ccgttccacg gtctgctatt gacgaccgcc ggtcctccgg
     9121 tagagaattc aaaaactttc tgcccaccta cttccttgcc cgagatggta gtcaggcctg
     9181 atttcatcgt gaatctgcat ttgattccgg cggaaagatc ttctttgaaa tcaaaaagcc
     9241 agttcttctc gtcggcccac ctttccgatc cgagttccgc gcaattcgta tcaaacggtt
     9301 tcacaggttg tttgagaaaa ccgaatggca ccatggcttc tgaaaattgt acgcgcacct
     9361 ggcg
//
LOCUS       DAORFF010000075         9291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3505_length_9291_cov_3.918038, whole genome shotgun sequence.
ACCESSION   DAORFF010000075 DAORFF010000000
VERSION     DAORFF010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3505_length_9291_cov_3.918038"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PKK76_04680"
     CDS             complement(<1..626)
                     /locus_tag="PKK76_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67793.1"
                     /translation="MKSKRFLLLSAIVPFLVLGLSLRVTQEEHPAGLAVSAPDFSPIV
                     SSLQTLQTGTLHLTTAQPAWKFVMDGPQKLHATSANDWFAVRQSATWANSNSTLADNL
                     IMSLKNSGLLKNSGTVQSTATVNGVVYKVKLQTGGCGANCTGVTAAAASGLNFTNRFK
                     MWRASDNANVLELMFNDVTNPTSGILLTYNLGILSPTVSDNTNLIVES"
     gene            complement(623..823)
                     /locus_tag="PKK76_04685"
     CDS             complement(623..823)
                     /locus_tag="PKK76_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67794.1"
                     /translation="MVRKMAIILMVCVLGLGSCAFLNLDKKEEKDNTNLLLLLFGAAY
                     LNSQGCKNNSGLVICIPPGVPN"
     gene            complement(916..1224)
                     /locus_tag="PKK76_04690"
     CDS             complement(916..1224)
                     /locus_tag="PKK76_04690"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNL67795.1"
                     /translation="MFRERVREKLSESQIESMLQDTPGWAREGESIRRVFAFSDFQRA
                     FSFMSGCALVCEKADHHPDWSNSYNRVDVRFTTHDAGGLTLKDFELARAFNAFLMQKA
                     "
     gene            1289..1966
                     /locus_tag="PKK76_04695"
     CDS             1289..1966
                     /locus_tag="PKK76_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67796.1"
                     /translation="MSRALLLLTLFAISCSSPYLRNRGRDALDIFTVEFQTRAYGASV
                     RAGPVKAGLYHKSADGAAAGLRGGDLGVHHDAEFAVLFFGADYFQKKPLGDLETPSEE
                     DSKTAEETDPSQTALRGKEFRARSPFGTTQPAQKKRSVFKKKGGFAPASYYTQLEVSL
                     GIFFGVKLGINPGELLDALLGWTTLDIFRDDEPFETEQEKALRDNPLYDRLTDEQRQK
                     LKEQMAK"
     gene            complement(1982..3103)
                     /locus_tag="PKK76_04700"
     CDS             complement(1982..3103)
                     /locus_tag="PKK76_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNL67797.1"
                     /translation="MNHRKCRGCSAVFAVTCLDNLLAAKITHLEILRQSLVLLEHGSA
                     RDKACAEQVHRRMNFAALGSVGPDVFYFYHVLSRKRNQLGLHWGNLSHHSRVFDLMIG
                     FLQRVKDMPEGIRREKCYAFAMGYISHCAVDIVTHPYIFYITGDYYSIDLKQAVRAQE
                     NHLRVEFQLDAYLVHHRWGLEPSRYNFAQYVDIHEETPDGPAMDYDLWSMWVDALAMV
                     YRDEFENTYFGSEARILPGDILNEAYVGFMKFNRILDTRSRPVRALLTAIDFATFHTI
                     KARYLLLPPPDQINPRLLNEERKEWKYPGDPQVTSKESFMDLVHRAAKFAADAMADAD
                     AFITGSKKLRDLAEKYDGYNLDTGLRSESIAMHEFDPID"
     gene            complement(3045..3674)
                     /gene="hisG"
                     /locus_tag="PKK76_04705"
     CDS             complement(3045..3674)
                     /gene="hisG"
                     /locus_tag="PKK76_04705"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNL67798.1"
                     /translation="MGVFTFALPAGRLALESIQFFRSSGLCDIQIPEGSRELCFTDPS
                     GEYRIILVRPTDVPTYIIQGGAHAGITGKDVLLESTFDVTTPLELGFGKCRLSVAGKP
                     DEKPLEKKHLRVATKYPVQAMNFLYQKGISCEIIKLHGSVEIAPALGLADCIVDLVST
                     GSTLRANGLVEQEVILNSEAVLVVGRFAYALESQKVQGMLSRFRSHLPG"
     gene            complement(3676..4383)
                     /locus_tag="PKK76_04710"
     CDS             complement(3676..4383)
                     /locus_tag="PKK76_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67799.1"
                     /translation="MRRKVITPEAVDPLRDFHGNQFERAVATAFYWMRQHSRQVVLGL
                     ILVVVVGTASLAFFIYRDYREQKSLTDFEELMKSPVMASGSGAEKVAIEKLEKYEREF
                     PNDHAQLRAGLKKVDLLIKTGEKEKAGDALVEIARIVETPELSALFSYRAGLLFEEKG
                     KFAKAESAYSNASQKLMDEGGAAALARFGQARALILLNKDREGREVMKKMLEIKDVES
                     IADLRIAAAAFLIQHGK"
     gene            complement(4385..6001)
                     /locus_tag="PKK76_04715"
     CDS             complement(4385..6001)
                     /locus_tag="PKK76_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNL67800.1"
                     /translation="MLGALDGQGDVQSGDLVEAVVSQCDEREVYLDVGGKTEGACPIA
                     EFSEPPPVGSRVSVVLVSRGGDQPPRFSRRLAEQRKAWEDIKEAFQSKARLSGKIQRV
                     VDHGFIVNVGQLDLFMPLSQSGLRPNKNLSVGSEIDFKILEIKDNKHSAIVSHRAILE
                     EKNEALWNDLTSKHHEGDVVAAKVARRVSYGLFAELCGIEGLVHQSDVSYKKNVSLRD
                     RFPIGAEIQVKILKMDRENNRLSLGIKQLQEDPWEWAQRELKVGDVVDGTIMNVTNYG
                     AFFEIREGLEGLIHVSDLTWSGKRENPGKLVEPGKPARAKILSIDPANKRISLGLKQL
                     EEDPFEQVVRGLKVGDTAEGVVSSITKFGAFVKLRDGVDGLIRYSDYSHDEPVDKKML
                     TAGQTVTFKVLEIDRGKRKITLGMKQLSPGPYELLKKKYKRGDVLDCTVRNVTQFGLF
                     VSFGEKQEALIHISRIPIPEGKKLEDLFKQGDQLKAVLTRIDSDDKKISMSILDYEKK
                     TERDIMNQYLKKEDGPSTSSLGAYMKNLKL"
     gene            complement(6089..6769)
                     /gene="cmk"
                     /locus_tag="PKK76_04720"
     CDS             complement(6089..6769)
                     /gene="cmk"
                     /locus_tag="PKK76_04720"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNL67801.1"
                     /translation="MKSTVITLDGPAGSGKSSVAKELARRLGMLHADSGGIYRTFTLA
                     LMRRLGPCPSHEAFGEALSQSSISPESLEVSILTDGTHQYNLIRGQDQADEIRSPDVT
                     SRIRYVADRPVFREAVNALLRGMTNVVADGRDMGSVVFPNASYKYYLEASVDIRAARR
                     AAENEARGWKEDLGEIKESIRKRDEEDEKREYGALIRTPDMILIDTGPLNLEGVIGRI
                     LNSLQIKF"
     gene            complement(6766..7656)
                     /locus_tag="PKK76_04725"
     CDS             complement(6766..7656)
                     /locus_tag="PKK76_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNL67802.1"
                     /translation="MIRFKSVWIHGMGMMGASLALALRKSSQFEGRVFGAVRSQKSAD
                     YIRSTDLCDEVHVVSSGREAREHFDSLPESSLIVIGVPVASAVSLLGDLRGIQHLVTD
                     MSSTRAEVEKASAGVRFVGSHPICGSEDTGPQAARPGLFETRLCIITPSPESRNEDIQ
                     VVSDFWKDLGMNVMSMTAPEHDRILAYLSHAPHVISGLMAHWGMSDAVAEAVRKSPVP
                     LTGGGFRDMVRIAGSNPEMWLDIIRTNRREVIGSLETFQKYLADTIGLLKGDDEQKMM
                     EWLRSARLKRNRLCGYPDDK"
     gene            complement(7653..8429)
                     /locus_tag="PKK76_04730"
     CDS             complement(7653..8429)
                     /locus_tag="PKK76_04730"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNL67803.1"
                     /translation="MKSNEIDRLREKIDQLDRSIVDLISQRTAVAEEIGQIKKRQDLP
                     IFRPDRHGAVYDRVADSVPEAMASGIRYIYREIMSLGMAVEGDLVFLCPDALSGAAAR
                     FAFGSSVEIALADAPGILRRVSADRNAFGFVPAGEADAVFESLLDGDVLCYGEVVLRP
                     DHRRYIILSTWNCPRSGRDKTSVAASGDLKALISCIEDAGREVLRVETFHSQKHQGPC
                     AFLDFPGHREDPDCAVILENIKSRSDYFHFLGSYPAAAEL"
     gene            complement(8416..8949)
                     /gene="scpB"
                     /locus_tag="PKK76_04735"
     CDS             complement(8416..8949)
                     /gene="scpB"
                     /locus_tag="PKK76_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNL67804.1"
                     /translation="MARYRGLIEAVLFVATEPQTVAMIARTINLDRVNTRILADSLVD
                     DFAERDGGISLREIAGGYQFVTSDRYSEEMREILQTSKREKLSRSVMETLAIISYRQP
                     ITLPEIEDVRGVASRPHVVTLLAKRLIKPQGTRPVPGRPTLYVTTKTFLQHFALNSLA
                     ELPPLEELKELKFDEIE"
     gene            complement(9054..>9291)
                     /locus_tag="PKK76_04740"
     CDS             complement(9054..>9291)
                     /locus_tag="PKK76_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67805.1"
                     /translation="RITEVSVEEKVAHIRQIFENAVSCLFTDLLVDLEQRGSGEVIAT
                     FLGILELTKLGEIVLRQKVNFGQIEVHKKSYVLV"
BASE COUNT         2355 a         2464 c         2163 g         2309 t
ORIGIN      
        1 taactttcta caatcaagtt cgtgttatct gaaacagtgg gcgaaagaat tcccagattg
       61 tacgtcagca ggatgccaga agtaggattg gtcacatcat tgaacatcaa ctccagcaca
      121 tttgcattgt cactggcgcg ccacattttg aaacgattgg taaaattcag gcccgacgct
      181 gcagcagccg taacacctgt gcaattcgca ccacagccac cggtctgcag ctttaccttg
      241 tagacaactc cgtttacagt agccgtgctc tgcaccgtcc cagaattctt gagaagtcct
      301 gaattcttga gactcatgat caggttgtct gcgagagtgg aattactgtt tgcccaggtt
      361 gcggattgac gaacagcaaa ccagtcattc gcactcgttg cgtgcaactt ctgaggacca
      421 tccattacga acttccatgc aggttgtgca gtagtgagat gaagagttcc agtttgcaga
      481 gtctgcaaac tcgaaacaat gggagagaaa tcgggtgcag acaccgcaag ccctgccggg
      541 tgttcttcct gagtgacgcg cagcgaaagc cccaggacca ggaatggaac gattgcagaa
      601 aggagaagaa aacgttttga tttcaatttg gaactccagg cggaatgcaa atcacgagcc
      661 ctgaattatt cttacacccc tggctattca gataggccgc cccaaacaag agaagaagca
      721 gattggtgtt atccttctcc tccttcttgt ccagattcaa gaaagcacag gatcccagtc
      781 caagcacaca aaccataaga atgattgcca tcttccgtac catactcacc tcaatacatc
      841 tgacgtaaaa tcgacgccga ttgtatttcc catcaagtaa atttctatgc aaaaaaaaga
      901 gtcccttgcg agacgttaag ccttttgcat taggaatgca ttgaatgccc gcgccagctc
      961 gaaatctttc agagttagcc caccggcatc gtgtgtagtg aaacgaacat ccacacgatt
     1021 gtaactattc gaccagtccg ggtgatgatc tgctttctcg catacaaggg cacagcccga
     1081 catgaacgag aatgctcttt gaaaatcaga aaatgcaaat acccgtcgaa tcgactcccc
     1141 ttcccgggcc caaccaggcg tatcctgcaa catagattcg atctggcttt ccgaaagctt
     1201 ttctctcact cgctcacgaa acacagcttc acacaccgtg gcgacaaatt atcggtgact
     1261 cccggtcctc cggctggacc cttgcccgat gagccgcgcg ctcctgttgc tcacactgtt
     1321 cgccatatcc tgcagctccc cctacttaag aaacagggga agagacgcac tggatatctt
     1381 taccgtggaa tttcagacca gggcctatgg agcgagtgtt cgcgccggcc cggtcaaagc
     1441 aggtctctac cataaatcgg ctgacggcgc agccgccggt ttgcgcggag gggatctggg
     1501 agttcatcac gatgcggagt tcgcggtcct attttttggc gcagactatt ttcagaaaaa
     1561 gccgttaggc gatctggaaa caccttcgga agaagactca aaaaccgcag aagaaaccga
     1621 tccgtcgcag acagcactcc gcggaaaaga attcagagca cggtcaccct ttggaaccac
     1681 tcaacccgcc cagaagaagc gctcggtctt taaaaagaaa ggtgggtttg cccctgcttc
     1741 ctactatacg caactggaag tctcactcgg aatctttttt ggcgtgaagc tcggcatcaa
     1801 tcccggagag cttttggatg cgctcctcgg gtggaccact ctggacatat tcagggacga
     1861 tgagcctttt gagacagaac aggaaaaggc attgcgagac aatccgcttt acgacaggct
     1921 cacggacgaa caaaggcaaa agctcaaaga gcaaatggca aagtgagaca gctttgggtg
     1981 gttagtcgat tggatcaaat tcgtgcatgg caatcgactc tgacctgagg cccgtgtcca
     2041 gattgtaacc atcgtatttc tcggcaagat cgcgcaactt ctttgaacca gtaatgaatg
     2101 cgtctgcgtc tgccattgca tctgcggcaa acttcgcggc cctgtgaacc agatccatga
     2161 aactctcttt cgatgtgacc tgtggatctc ctggatactt ccattccttt ctctcttcat
     2221 tgagtaatct tggattgatc tgatccggag ggggcaagag caaataacgg gctttgattg
     2281 tgtgaaaggt tgcaaagtcg attgccgtga ggagcgctcg caccgggcgc gaacgagtat
     2341 ccagaatgcg attgaacttc atgaatccaa catacgcttc gttgagaatg tcccctggaa
     2401 gaatgcgagc ctctgagcca aagtaagtat tttcaaattc gtcgcggtaa accatcgcaa
     2461 gagcatccac ccacatggac cagagatcat agtccatcgc cggaccatcc ggagtctctt
     2521 catggatgtc cacatactgt gcaaaattgt accgggatgg ttcgagtccc cagcgatgat
     2581 gcaccagata cgcgtcgagt tgaaattcaa cgcgaagatg attctcctga gcgcgaactg
     2641 cctgtttgag atcgatggag tagtagtccc cggtgatata aaatatgtag ggatgtgtta
     2701 caatatccac cgcgcagtgc gaaatgtacc ccattgcaaa agcgtagcat ttttcgcgac
     2761 ggatcccttc tggcatatct ttgacccttt gcagaaaccc gatcatcaaa tcaaacacgc
     2821 gagaatgatg agacaaattg ccccagtgta gaccaagctg gttgcgtttg cgcgaaagaa
     2881 catgatagaa gtaaaaaaca tctggcccga ctgatccaag agccgcaaaa ttcattctcc
     2941 tgtgaacctg ttcggcacat gccttatcgc gcgcagatcc atgctccagc aaaaccagag
     3001 actggcggag aatttccaga tgtgtgatct ttgcagccag aagattatcc aggcaggtga
     3061 ctgcgaaaac ggctgagcat cccctgcact ttctgtgatt caagagcgta agcgaatctt
     3121 cctacaacca ggaccgcttc gctattcaaa atgacttctt gctccacaag accattggcg
     3181 cgaagagtcg atccggtgct cacaagatcc acaatgcaat ccgcaagacc cagagcgggc
     3241 gcaatttcaa cggaaccatg aagctttatg atttcgcacg aaattccttt ctgatatagg
     3301 aagttcattg cctgcactgg atacttggtc gcgacacgaa gatgcttttt ttcaagaggt
     3361 ttttcgtccg gctttcccgc aacagacagt ctgcatttcc caaaaccaag ctcgagtggt
     3421 gtagtcacat caaacgtgct ttcgaggagt acatcttttc cagtaattcc agcgtgggcc
     3481 ccgccctgaa tgatgtaggt aggaacatct gtgggccgaa ccagaatgat tcgatattcc
     3541 ccggatggat ccgtgaagca cagttcgcgc gacccttctg gaatctgaat gtcgcataga
     3601 ccgctggaac gaaaaaattg aatgctttcg agcgcaagtc gtcccgctgg cagagcgaat
     3661 gtgaaaacgc ccatattact ttccgtgttg aatcaaaaat gccgcggccg caatgcgaag
     3721 atcagcaatg ctttctacat ctttgatctc gagcattttt ttcatgactt cgcggccctc
     3781 gcggtctttg ttcagcagaa tcaatgcacg cgcctggcca aatctcgcaa gagcagcagc
     3841 tccgccttcg tccataagct tctgcgatgc attggaatag gcagactctg ccttcgcaaa
     3901 cttgcctttc tcttcaaaca aaagacccgc tctgtaggaa aacagcgcag aaagctcggg
     3961 cgtttccacg atgcgcgcaa tttcaacaag cgcgtcccct gctttctctt tttcgcctgt
     4021 cttgataagg agatctacct ttttgagacc tgcgcgaagc tgggcgtgat cgtttggaaa
     4081 ttcgcgctca tacttctcaa gcttttcaat ggcaaccttt tcagcgccac tgcctgatgc
     4141 catcacaggg cttttcatca gttcctcaaa atcagtgagg ctcttttgct cgcgataatc
     4201 ccggtaaatg aagaatgcaa ggctggcagt cccgacgacc acaaccagaa tcaagccaag
     4261 cacaacctgt cttgaatgct gacgcatcca gtaaaatgcc gtagcaacag cgcgctcaaa
     4321 ctgattcccg tggaaatcgc gtaacggatc aactgcttct ggagtaatga cttttctacg
     4381 catatcagag cttcaggttt ttcatgtaag cacccagact gctcgtagac gggccgtcct
     4441 cttttttcag gtactgattc atgatgtctc tttctgtctt cttctcatag tcgaggatag
     4501 acatggaaat ctttttgtcg tcggaatcaa ttcgtgtgag cactgctttg agctggtctc
     4561 cctgcttgaa caggtcttca agcttcttgc cctctggaat tggaatgcgc gatatgtgaa
     4621 tcagcgcctc ttgcttttct ccaaaggaga caaacagacc aaattgagtc acattgcgaa
     4681 cagtgcaatc gaggacatct ccacgtttgt atttcttttt gagaagttca tacgggcctg
     4741 gcgaaagttg cttcattccc agagtgatct tgcgcttgcc gcgatcaatt tcgagaacct
     4801 tgaaggtgac agtttgccct gctgtgagca tcttcttgtc tacaggctca tcatgggaat
     4861 aatccgaata tcgaatgagt ccgtccacgc catcgcgtaa cttcacgaag gcgccaaact
     4921 tggttatgct ggaaactaca ccttctgctg tgtctcctac tttcagacca cgaaccacct
     4981 gttcaaatgg atcttcttcc agttgcttga ggccaagtga gattctctta tttgcaggat
     5041 caatcgagag tattttcgct ctggctggct ttccaggttc aaccagtttg cctggatttt
     5101 cgcgcttacc ggaccatgta agatccgaaa catgaatgag accctccagg ccctcgcgaa
     5161 tttcaaaaaa tgcaccgtaa tttgtgacgt tcatgatggt tccatccacc acatcgccta
     5221 ctttcagttc tctttgcgcc cattcccagg gatcttcctg caactgcttg attcctagag
     5281 agagtctgtt gttttcgcgg tccatcttca ggatcttaac ctgaatttct gcaccgatag
     5341 gaaagcgatc gcgaagagaa acattcttct tgtaagaaac atctgactga tgaacgagcc
     5401 cttctatgcc gcagagctcc gcgaagagtc cataggaaac cctgcgcgcc actttcgcgg
     5461 caacaacatc accttcgtgg tgtttgctgg tgagatcatt ccagagtgct tcattcttct
     5521 cttcaagaat ggcacggtgg cttacaattg cggaatgctt gttgtctttg atttctaaaa
     5581 tcttgaaatc aatctcagaa ccaacagaga ggttcttgtt cggacgcagg cccgactgag
     5641 acagaggcat gaaaagatcg agctggccta cgtttacaat gaatccatga tctacgacgc
     5701 gctgaatttt tccgctcaga cgcgctttgg actgaaacgc ttctttaata tcttcccagg
     5761 cctttctttg ttccgcgagt ctgcgggaaa aacgaggagg ctgatctcca ccgcgtgata
     5821 cgagcaccac ggatacgcga gatccaacag gaggtggttc tgagaactca gcaatcggac
     5881 aggcgccttc cgtttttccg ccaacgtcca gataaacttc acgttcatcg cactggctga
     5941 caactgcttc taccagatcc ccgctttgaa catctccctg gccgtctagc gcgccaagca
     6001 tgtcctccat cgacttaatc tctttctgat ccaggtcaaa gggtgtcgtg cttttcgatg
     6061 tcatgaaaat ggaattgtga gaggaggttt aaaacttaat ctgcagactg ttgagtatgc
     6121 gtccgataac accttccaaa tttagagggc cggtgtcaat tagaatcata tctggagttc
     6181 gtatcaatgc cccgtactcg cgtttttcat cttcttcatc gcgtttacgg atgctttctt
     6241 tgatttcgcc cagatcttcc ttccaaccgc gggcctcatt ctcagccgct ctgcgcgcag
     6301 ccctgatgtc aacggacgct tccagataat acttatacga tgcatttgga aaaactacac
     6361 tgcccatgtc gcggccgtcg gccacgacgt tggtcatccc gcgcaaaaga gcattcaccg
     6421 cctcacgaaa gaccgggcga tcggccacgt atcgaatccg ggaagtaaca tctggagaac
     6481 gaatctcgtc tgcctgatcc tgaccgcgaa tcagattgta ctgatgtgtt ccatccgtaa
     6541 gtatactgac ttcgagagat tccgggctta tggacgattg agaaagtgcc tctccgaatg
     6601 cctcatgcga aggacacggc cccagtcttc tcatcaatgc aagtgtaaac gttctataaa
     6661 ttccgccgga gtccgcgtgc aacataccca gacgacgcgc aagctccttg gcaacgctac
     6721 tcttccctga accggcaggg ccatccagtg tgatgactgt tgatttcatt tgtcgtcagg
     6781 atatccgcaa agacgatttc ttttgagtcg agcagaacgc agccattcca tcatcttctg
     6841 ttcgtcatcg ccttttaaga gtccaattgt gtccgcgaga tatttttgaa atgtttcgag
     6901 cgacccgatc acttcgcggc gatttgtgcg aatgatatcc agccacattt ctggattgct
     6961 tccagcgatc cggaccatgt ctctaaagcc gccgccagtt aatggcaccg gagattttcg
     7021 cacggcttcc gctacagcgt ctgacatgcc ccagtgagcc atcaggccgg aaatcacatg
     7081 aggggcgtgc gaaaggtagg cgagtatgcg atcatgctcc ggcgcagtca tggacatgac
     7141 attcataccc aggtccttcc agaaatcgct cactacttga atgtcttcgt ttctggactc
     7201 aggagaagga gtgatgatgc agagtctggt ttcaaacaat ccaggacgcg cagcttgagg
     7261 tccggtatct tctgaaccgc agattggatg agatccaaca aatcgcacac cggcagatgc
     7321 cttttccact tccgccctcg tagatgacat gtctgtcacc agatgttgga ttccgcgaag
     7381 atctcctaga agggaaaccg cagaggcaac aggcacacca attacaatga gagaagattc
     7441 tggcagagaa tcaaaatgct cacgggcttc tctcccactt gatacaacgt gcacttcatc
     7501 gcataaatct gtagaacgaa tgtaatccgc gcttttctga ctgcgaaccg cgccgaacac
     7561 gcgaccttcg aattgcgaag acttgcgaag ggcgagcgca agagaagcgc ccatcatgcc
     7621 catgccgtga atccatacgg acttaaaacg aatcaaagct ctgccgccgc aggataggac
     7681 ccaagaaagt gaaagtagtc cgaacgagat ttgatgtttt cgagaatcac agcacagtca
     7741 gggtcctctc tgtgaccggg aaagtcaaga aacgcgcacg ggccctggtg tttctgcgaa
     7801 tgaaacgttt ccactcgcaa aacttctctc cccgcatctt caatgcagct gatgagtgct
     7861 ttcaaatctc cgctggcggc aacgcttgtt ttatctcgtc cagatcgcgg acagttccac
     7921 gtggaaagaa tgatgtatct tctgtgatcc gggcgaagaa ccacttcgcc atagcaaagc
     7981 acatctccat cgagcaggga ttcaaagacc gcgtccgctt ctccagcggg aacaaatcca
     8041 aatgcatttc gatctgcgga aacgcggcga agaatcccgg gcgcgtctgc cagagcaatt
     8101 tccaccgagg acccaaatgc aaaacgagcc gccgctcccg aaagagcatc cggacacaag
     8161 aaaaccagat ccccttccac tgccatacca agagacatga tttcgcggta aatataacga
     8221 atgccagaag ccattgcctc tggaactgaa tcagccacgc gatcatacac ggcgccatgc
     8281 ctgtcggggc gaaagatggg aaggtcctga cgcttcttga tttgtccgat ctcttctgcg
     8341 acagcagtac gctgcgaaat caaatcgact atggagcggt cgagttgatc aatcttctct
     8401 ctgagcctgt cgatttcatt cgatttcatc gaacttcaat tcctttagtt cttccagggg
     8461 tggcaattct gccagagagt tcaacgcgaa atgttgcagg aaagtcttcg tggttacgta
     8521 aagagtaggt cttccgggga caggtcttgt tccctgtggc ttgatcagtc tctttgcaag
     8581 cagagtcacc acatggggac gcgaagcgac gccgcgcaca tcttcgattt ctggaagagt
     8641 gataggctgg cgatatgaaa taattgcaag tgtttccatc acagaccgac tcagcttttc
     8701 tctcttgctg gtctgaagaa tctctctcat ttcttcgctg tagcgatctg atgttacaaa
     8761 ctgataaccg ccagcaatct cacgaagact gatgccgccg tctctttctg caaagtcatc
     8821 cacaagactg tccgcaagaa tccttgtatt gactcgatcc agattgatgg tgcgagcaat
     8881 catggctaca gtctggggct cagttgccac aaacaaaacc gcttcgatga gaccgcgata
     8941 acgtgccatc tcctccgcac ccaggtccct gaaggccgga agccctgcgt catcgcctga
     9001 aatgtgaagt tctactttct cttcgccagt ctgttctttg tcttcagcca tgatcaaaca
     9061 agtacatatg attttttgtg aacttctatc tgcccgaaat tcactttttg ccgcagcaca
     9121 atttcgccca gctttgtaag ttcaagaatt ccaagaaacg tggcgatgac ttcacctgaa
     9181 ccgcgctgct caagatctac aagcaagtcc gtgaacaaac aggaaaccgc gttctcgaaa
     9241 atctgtctga tatgtgctac tttttcttcc acgcttactt cagtgattcg g
//
LOCUS       DAORFF010000076         9261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3523_length_9261_cov_3.450902, whole genome shotgun sequence.
ACCESSION   DAORFF010000076 DAORFF010000000
VERSION     DAORFF010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9261
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3523_length_9261_cov_3.450902"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PKK76_04745"
     CDS             complement(<1..1074)
                     /locus_tag="PKK76_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67806.1"
                     /translation="MRIKHSFFYYRAIFQFSLREEVSIFFEIVRPTFRPLFLSLIFAL
                     TQALHAAPDKDAKKDDSGFYTFGLQLMFWTGLNRDFYSSDLETRFFRDDILRGQNTAQ
                     PVAAAQYNNKWVSKPQFNLPVRGTFSPGFLPQLNLEGAYYRERGNITYYGLSDLPAPD
                     RTHIVELSGFDRSTTTFGAAYNFLGYFRVDPMSLLALRGGYIRESTDFNFKPLSFATS
                     GVIVSLFDNPFHAVASGWYGGIELTLPIWQKWSATLKVDRSEALHGNMQYEKIQLQPA
                     SGGIQATYDRAESRYRLARTDRMAAVNYHITSDFKVTVGYREVIYDVSYPGFFSYSLT
                     NAGGAVQVFPVTEFILDYFNYQET"
     gene            1094..2311
                     /locus_tag="PKK76_04750"
     CDS             1094..2311
                     /locus_tag="PKK76_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67807.1"
                     /translation="MSPSAWRELNRAESSFWLLSEVSSMNFAVFAKCKGEVSDAALSA
                     VCDQMMDSYPMAQASVDVSSGKPVFVRAEGKVAVKRTSGDWTQDFARELILPFSFPGA
                     FWRVTVHDLERAVFQIAITFHHSVADGRSGFSFMLDFLRRLDQYPAQLETERREFPSD
                     RFAPFLRNAKSPRLTTDSMPWFAKRSQPSSPQMDCFTLEKEETSRLVSRARENKCTVH
                     GWIGAAQILSLSSLFGDDRSHTLALSTPADVRSRLYGGAPDAGLELCISLVTTAIAAT
                     EMGTVARELSQHIRSELASDAFLQFYEALPDPVTLLSNPKGLTLFATMMQRGVQASVL
                     SNVGIVSPPVFQKIAVESMSFTVHPMITQPVFVTVTTFEGRMHFVLNHDSNRWESRTF
                     DSFLESFRNSLAG"
     gene            complement(2315..2752)
                     /locus_tag="PKK76_04755"
     CDS             complement(2315..2752)
                     /locus_tag="PKK76_04755"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNL67808.1"
                     /translation="MPKPVPFAFSIPMKVAWGDMDAFGHVNNVSYARYFETARAEFFS
                     SFRLWAAKLDKQSSGPVLIHMDLDYRKQTVYPETLSITVGITSASRRSFSMGCSMWNE
                     KQECVLTATADFMWFDFDAGKPSTIPEQFHKLALAEAERRKER"
     gene            2878..5805
                     /locus_tag="PKK76_04760"
     CDS             2878..5805
                     /locus_tag="PKK76_04760"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNL67809.1"
                     /translation="MRNLISRFCDFYLRRPLWSGLVAMVLLAASVYFAYQVKINSNQL
                     DLLPSDLPEVIEARRVTDMIGGTGYVLVTLRPTDRDAGDTLFDKALTATRQSRDKEAV
                     EFLAQANVEYKKLMPVNLKKAELLKKASDELNTELKQLPETQYVLYKADLRAIKERIL
                     YFVKTPDLKEAFRRISIKRDEMIERSSPFYIDLGQPEYKLELSDLIEKYKRIGKKEIT
                     DDYYVSPDRKMMTLIIKPRFSMHEIQKSRDYVAKIREIVARNKFGERGIEVGMTGAYV
                     LYVDAYDSIENSSKPTLIWALIGIAVILVVFVRRPSYILAMLLSLVYSIVIVYGMTYL
                     IIGELNLITSIFGGILAGLGIDFGIQLIFRFREEYDQNPDFVPAMTGAILHTGTAAFF
                     AVLTMTAAFATLIISRFKGFSEFGMISAYGIVITFLCMFFLTPLILAIFQRIFPNLLK
                     RHAREDQLHDQEVEERWHKRVNVPRIARILLAIMVLSVIASSFGFTRVKWDNDSRNMI
                     DYNIASERLQEEIALRYELTGSPAVIATKSLDEARALFEHFEPPTPAVADIVAQTISI
                     WAYVPPYQQQVQNSELMQKFRADSSQIKPGILPPQYRHFYPIYQTIIGQKPFGVKDLP
                     KDTISQFESKPESKEKGWLTFFYPVASKLYTVDGLNQMERQTGKISVPIVGRQSLKSL
                     AYFAPKLEVALRKKAGGDAAKKVSLDGMEFTEREVSLALFAANSGDQKFLKSTGLSDL
                     AIETILKHRPFANVEALQNEKLSVQGTGSVLIVARFANLVLEESKTIILMSLSLVVAL
                     LYLSFRDLRSTLIALVPMIAGLVLMIGLMAIFNVKVNYFNVAVLPIVIGYGINNGFFL
                     FYRFMESGSIFQTMFHTGVAGLGSALTSLAGWGAIASATHPGLRSMGYLAFLGLSAMT
                     IAMLVFLPALLALLNSRYPALIERLRLRHGGTAKADPSEIEVPVAVTSAAEGSTKRAK
                     KGK"
     gene            complement(5812..6498)
                     /locus_tag="PKK76_04765"
     CDS             complement(5812..6498)
                     /locus_tag="PKK76_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HNL67810.1"
                     /translation="MKVQKKRIAELESFVVEGKDKGPVVVLCHGFGANAQDLLPLAEA
                     LSAPAGTTWIFPDAPIEFPIGPGRMGRAWFPLVAEEIARLAATGKHLNFYEVVPPGLE
                     ASRKKLEALIHETGRPANLITLGGFSQGSMLAVDTYLHASETMAGLVIMSGNLICRDQ
                     WTRLAPARRGSQFIQSHGLMDPVLPYENAKRLETVLREGGLSGEFISFSGGHEIPQGV
                     IKKVSLYLTR"
     gene            complement(6495..7031)
                     /locus_tag="PKK76_04770"
     CDS             complement(6495..7031)
                     /locus_tag="PKK76_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNL67811.1"
                     /translation="MMPEIFTSRIKLRAFRESDLDEFAAMNADERVMKYFPATLTRQE
                     TIELMRRIDDHEKKHGFTLFALEDISTGKFFGFTGAAVPAFDAHFTPCVEIGWRLAFD
                     FQGKGLATEAALATVNYAFAHLGVSELVSFTFKGNLPSRRVMEKIGMAYHPEEDFLHP
                     QLAREHHLAPHVLYRRKR"
     gene            complement(7041..7475)
                     /locus_tag="PKK76_04775"
     CDS             complement(7041..7475)
                     /locus_tag="PKK76_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67812.1"
                     /translation="MSFAQDNKSENDRLRAIMARLTDHHWSLDLGGDWTVGTLLCHLA
                     YWDRMTSVRIKKWMGDGTLTAVPDADNIECINNSVRFLSRGVGRVEGIRTVQEAADEI
                     DSIALSLSPDQLNFLEANGRDRWFRRSLHRQHHLARIEKALL"
     gene            complement(7556..8419)
                     /locus_tag="PKK76_04780"
     CDS             complement(7556..8419)
                     /locus_tag="PKK76_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67813.1"
                     /translation="MIRTTLLVLTFAVCLGLHAEETSPVQLEWQSFRIFSTGSDDRHL
                     LVFQKKKDPGPLLRLQAWDYGHQRIMNVAPMRGTSGELLYAQTFDRGESGGSITLTFF
                     KASGDAVQPLPFEIARSGYNAPEVQSGSGPSRILIPCEAFYGKRVGPFSLTGVYVSGH
                     GLSMIPRIAVMDQAQGKVNLKDVTFSTGVRNVLNGFLVQSEKALGEQSGKTWEGAEGL
                     QLAAGLATYYYFKIKLGEEADALAKITRAGIKILDSDQEKSSPAIHAADWLKTSRADI
                     VRFGDAEKLAE"
     gene            complement(8416..8847)
                     /locus_tag="PKK76_04785"
     CDS             complement(8416..8847)
                     /locus_tag="PKK76_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67814.1"
                     /translation="MSKETLSFISRTAQDDIFEVVFASYLKKDFISRALTLIHDSKLI
                     MRSLRFHSNDGGIAVWENGKGIISANSWEDEDAGIDPDSLMRFRFPLLNGAIAPDLAR
                     ETIIHDVISHLPNGELASILLKISKTKKWKEPLPSSYLEIL"
     gene            complement(8903..>9261)
                     /locus_tag="PKK76_04790"
     CDS             complement(8903..>9261)
                     /locus_tag="PKK76_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67815.1"
                     /translation="DCMGDQFILRDGMVYRLTLETAFLDPLGLSLDGFFASANEDPVE
                     FLQMHPLLRLEKEGKKLEPGQLIHAFPPYCTKESAQGVSLKAVEANAQIDFELDFFRQ
                     IRDIEDGGQIRIRVID"
BASE COUNT         2199 a         2288 c         2362 g         2412 t
ORIGIN      
        1 ggtttcctga tagttgaagt agtccagaat gaattctgta actggaaata cctggactgc
       61 accgcctgcg tttgtgagag aatagctgaa gaagcccggg taactcacgt cgtagattac
      121 ttctctgtaa cccacggtca ctttgaagtc agacgttatg tggtagttga cggcggccat
      181 tctatctgtt cgcgcaaggc gatatcgcga ctcagctcgg tcgtaggttg cctgaatccc
      241 accgcttgca ggttggagct ggatcttttc gtattgcata ttgccgtgaa gcgcttcgct
      301 tctatccact ttcagagtgg cggaccattt ctgccatatg gggagagtta actcaatgcc
      361 accataccat ccggatgcga ctgcgtggaa tggattgtca aagagggaaa caatgacgcc
      421 tgacgttgca aaagaaagag gtttgaaatt gaagtcagtg ctttctcgaa tgtatcctcc
      481 gcgaagggca agaagggaca tcgggtccac tctaaagtaa cctaaaaaat tatacgcggc
      541 gccaaacgtg gtggtggacc tgtcaaaacc gctgagttct acgatgtgag ttcggtcagg
      601 cgcgggcaga tccgacaatc cgtagtacgt aatattcccg cgctcgcggt agtaggctcc
      661 ttcgaggttc aactggggaa gaaatccagg agagaatgtt cctcgcacag ggaggttgaa
      721 ctgtggtttg cttacccatt tgttgttgta ctgtgccgct gccactggct gggcggtgtt
      781 ttggccacgc aagatatcat cgcgaaagaa gcgcgtttcg agatccgagc tgtagaaatc
      841 tcgattgagg ccggtccaga acatgagctg aaggccaaac gtataaaagc cagaatcatc
      901 cttcttcgcg tctttgtccg gggctgcgtg cagtgcctgg gtaagggcga aaatgaggga
      961 aaggaagagt ggtctgaatg tgggtcgcac aatctcaaaa aagatggaaa cctcttcgcg
     1021 caaggaaaat tgaaagatcg cgcgatagta aaaaaacgaa tgcttgattc tcattcgcaa
     1081 gaagcattgc acaatgtccc catctgcatg gcgtgaactc aatcgggctg aatccagttt
     1141 ctggctcttg agcgaagtct cctctatgaa cttcgctgta tttgcgaaat gcaaagggga
     1201 agtttctgat gcggctcttt ctgctgtctg cgatcagatg atggattctt atccaatggc
     1261 tcaggcgagc gttgatgttt cttctggcaa acctgtcttt gttcgcgcag agggaaaggt
     1321 tgctgtgaag cgcaccagtg gcgattggac acaagacttc gcgcgtgagt tgattcttcc
     1381 attctcgttt ccaggtgcat tctggcgagt gaccgttcac gatctggaaa gggcagtctt
     1441 tcagatcgcc atcacgtttc atcatagcgt ggcagatgga aggtccgggt tttcattcat
     1501 gctggatttc ttgcgtcgcc tggaccagta tccggcgcaa ttagaaacag agaggagaga
     1561 atttccatcg gatcgttttg ctccttttct tcgcaatgcg aagagccctc gtttgacaac
     1621 agactccatg ccctggtttg caaagagatc gcagccttct tctccgcaga tggattgctt
     1681 caccctggag aaggaggaga cttccaggct cgtgtcgcgg gcgcgcgaga acaaatgcac
     1741 ggtgcacggc tggattggtg cggcacaaat tctttctctt tctagcttat ttggagatga
     1801 tcgcagtcac accctggctc tttcaactcc ggcagacgtt cgctcgaggc tctacggcgg
     1861 tgccccagat gccgggttgg agctgtgcat tagcctggtc acgactgcaa tcgcggccac
     1921 agaaatggga acggttgcgc gagaactttc tcagcacata agaagtgaac tggcctcaga
     1981 tgcgtttctg caattctacg aggcacttcc tgatccggtc actctccttt ctaatccaaa
     2041 gggccttaca ctgtttgcaa cgatgatgca gcgcggtgtt caggcaagcg tactgagcaa
     2101 cgttggaatt gtgagtcctc ccgtgttcca gaagatagca gtggaatcca tgagctttac
     2161 tgtgcatcct atgatcacgc aaccagtctt tgtcacagtc accacgtttg agggccggat
     2221 gcattttgtt ttgaatcatg actcgaatcg ctgggagagc cgcacgtttg attcttttct
     2281 agagtctttt cgcaattccc tggcaggata gcgcctaacg ttcctttctt ctttccgctt
     2341 ccgccagagc gagtttgtgg aattgttccg ggatcgtaga tggttttccg gcatcaaaat
     2401 caaaccacat gaaatctgcg gttgcagtca gtacacattc ctgcttttca ttccacatag
     2461 aacatcccat ggaaaaagat ctacgggaag cacttgtgat gcccactgtg atgctgagtg
     2521 tttcgggata gacagtttgt ttgcgatagt ccaggtccat atggatgaga acaggtccgg
     2581 aggactgctt gtctagcttc gcggcccaca gacgaaagct ggaaaagaac tctgcgcgag
     2641 ccgtttcaaa ataccgcgcg tagcttacgt tgtttacgtg gccgaatgcg tccatatcgc
     2701 cccaggcgac tttcatcggg atgctgaagg caaatggaac cggctttggc atctcgcagg
     2761 gttctaagtg cgaagcatcc atcaagcatt attcacggaa gccctcggag cggaaactta
     2821 gagtgggatg caaatgaatt gcgcaacagg gccgtcgtaa aatgtttcga tcatatcatg
     2881 cgaaatctga tttcccgttt ttgtgacttc tatctgaggc ggccgctgtg gagcggactc
     2941 gtcgccatgg ttctgctggc tgcgtctgta tactttgcct accaggtgaa gatcaactcc
     3001 aaccagctgg acttactgcc gtctgatctt ccggaagtca tagaagcaag gcgagtcacg
     3061 gatatgattg gcgggacggg ttatgttctg gtgaccctga gacctacgga ccgcgacgcc
     3121 ggggatacac tctttgacaa agcgctcact gcaaccaggc agagccgcga caaggaagct
     3181 gtggagtttc tggctcaggc aaacgtagag tataagaaac tgatgcctgt gaatttgaag
     3241 aaggctgagc ttctgaagaa ggcaagcgat gagctcaaca cggagctcaa gcaattgccc
     3301 gaaactcagt atgttctata taaagcggac cttcgcgcga tcaaggaacg cattctctat
     3361 ttcgtaaaga cgccggacct gaaggaagcg tttcgtagga tttcgatcaa gcgcgacgag
     3421 atgattgaaa gatcgagtcc cttctacatc gatctaggcc agccggagta caaactggag
     3481 ctctctgatc tcatcgaaaa gtacaaacgc attgggaaaa aggaaatcac agacgactat
     3541 tacgtttccc cggatagaaa gatgatgact ctgatcatca aacctcgctt cagcatgcat
     3601 gagattcaaa agagcaggga ctacgtagcg aagattcgcg aaattgtagc acgaaataaa
     3661 ttcggagaaa gaggaatcga agtcggcatg accggagcct atgttctcta cgtagacgca
     3721 tatgattcca ttgaaaattc gtccaagccc actctgatct gggctctcat tggaattgca
     3781 gtgatcctgg ttgtgtttgt ccgaagaccc agctacattc tggccatgct tctgtctctg
     3841 gtctattcca ttgtgattgt ttacggaatg acctacctca tcattggaga actcaatctc
     3901 atcacttcca tctttggtgg gattctagca gggcttggaa tcgattttgg aattcagttg
     3961 atctttcgct tcagagaaga gtacgatcag aacccagatt ttgtgccagc aatgacagga
     4021 gccatcctcc atactggaac ggctgcattc ttcgcagtgc tcaccatgac cgctgcattt
     4081 gcgacattga tcatttctcg cttcaaaggt ttcagcgaat ttggaatgat ttctgcgtac
     4141 ggaatcgtca ttacctttct atgcatgttc tttctgactc ctttgattct ggcaattttt
     4201 caaagaatct tccccaatct actcaagaga catgcgcgcg aagaccagtt acacgaccag
     4261 gaagtggagg agcgctggca caagagagtg aatgttccgc gcattgcgcg cattttactc
     4321 gcaatcatgg ttctgtctgt gatcgcatct agctttggtt tcacgcgcgt gaagtgggac
     4381 aatgactcca gaaacatgat cgactacaac atcgcctccg aaagactgca ggaagaaatc
     4441 gcccttcgct atgaactgac aggttctcct gccgtgattg cgaccaaatc cctggatgag
     4501 gcccgcgcgc tgtttgaaca ctttgaaccg ccgactcctg cagtggcgga cattgtagcg
     4561 cagacgattt ctatctgggc ttacgttcca ccttaccagc agcaggtgca gaatagtgag
     4621 ctgatgcaga agtttcgtgc ggactcaagc cagattaagc caggcatctt gcctccgcag
     4681 tacaggcatt tctatccaat ctatcagacg atcatagggc agaagccgtt tggagtcaaa
     4741 gaccttccga aagataccat cagccagttc gaaagcaagc cggagtccaa agagaagggc
     4801 tggttgactt tcttttatcc ggttgcaagc aaactctaca ccgtagatgg cctcaatcaa
     4861 atggagagac agaccgggaa aatcagtgtt cctattgtgg ggcgtcagtc tctcaagagc
     4921 ctcgcgtact ttgctccaaa actcgaagtg gcgcttcgca agaaagcagg cggagacgca
     4981 gcgaagaagg tgagcctgga tggaatggaa ttcactgaaa gagaagtatc gctcgccctg
     5041 tttgcagcca attctggaga tcagaagttt ttgaaatcaa cgggactctc tgatcttgcg
     5101 attgaaacca tcctcaagca cagaccgttt gcgaatgtag aggcattgca aaatgaaaag
     5161 ctgtctgtgc agggaaccgg atccgttttg attgtagctc gatttgcgaa tctggttctg
     5221 gaagaatcca aaaccatcat cttgatgagt ctttctcttg tggtggcttt gctctatctt
     5281 agctttcgcg acttgcgctc tactctgatt gctctggttc ctatgattgc aggccttgtg
     5341 ctgatgatcg gtctgatggc catattcaat gtcaaagtga actacttcaa tgtagcggtt
     5401 ttacccattg taatcggata cggaatcaac aacgggtttt tcctcttcta tcgttttatg
     5461 gaatctggct ccatcttcca gaccatgttc catacgggtg tggccggtct gggctcggcg
     5521 ctgacttctc tggcgggctg gggggccatc gcatcggcca ctcatccagg gcttcgctct
     5581 atgggctatc ttgctttcct tggcctcagt gccatgacga ttgccatgct agtgtttctt
     5641 cctgcacttc ttgctcttct caactcccgt tatccggcat tgatagagag actgaggctc
     5701 agacacggtg ggactgcaaa ggcagacccg tctgaaatcg aagtgccggt ggctgtgact
     5761 tcagctgcgg aaggatctac aaaacgcgcc aaaaaaggaa aatgattccg atcagcgcgt
     5821 gaggtacagg cttactttct ttattacacc ctgcggaatc tcatggccgc cggagaaaga
     5881 aatgaattct ccggataggc cgccttcgcg aaggaccgtt tcgagtcttt tcgcattttc
     5941 gtagggtagc actggatcca tcaatccatg agattgtatg aactggcttc ctcttcgcgc
     6001 tggtgcgagt cgtgtccact gatcgcgaca gattagattt ccagacatga tgaccagtcc
     6061 tgccatggtt tcagaagcgt gaaggtatgt gtctacggca agcatcgacc cctggctaaa
     6121 gccgcctaac gtgatcagat tcgcaggtct cccggtctcg tggatcagag cttctagttt
     6181 ttttctggat gcttcgaggc ctggaggaac tacttcataa aaattcagat gctttccagt
     6241 cgcggcaagc cgtgcgattt cttctgcgac cagtggaaac caggctctgc ccattcttcc
     6301 tgggccgatt ggaaattcaa tgggtgcgtc cgggaagatc catgtagtgc cggccggcgc
     6361 gctgagcgct tctgccagtg gcaatagatc ctgcgcattt gccccaaaac catgacatag
     6421 aacgacaacc ggacctttgt cttttccctc tactacgaaa ctctccagct cagcgattct
     6481 ctttttttga actttcaacg ttttctccgg tacaatacgt gtggtgcaag atgatgctcg
     6541 cgcgcgagtt gaggatgaag aaagtcttcc tctggatggt aagccattcc aattttttcc
     6601 atgacgcgac gagaaggcaa gttgcctttg aatgtaaaag atacgagttc actcactccc
     6661 agatgtgcga atgcatagtt cacggttgca agagctgctt ccgtggccag gcctttgccc
     6721 tgaaaatcaa acgcaagtct ccagccaatt tccacacacg gagtgaagtg cgcatcaaaa
     6781 gcgggaacgg cagctccggt gaagccaaaa aacttaccgg tggaaatatc ttcgagcgcg
     6841 aagagagtaa acccgtgttt cttttcatga tcgtcgattc gcctcatgag ttcgatggtt
     6901 tcctgtctgg tcagagtggc cggaaaatac ttcatcaccc tttcatctgc gttcatggcc
     6961 gcaaattcat ccaggtcgga ctcccgaaat gcacgcaatt tgatcctgga agtaaaaatc
     7021 tccggcatca tgctcatgca tcaaagcaga gctttttcga ttcttgcgag gtgatgctgc
     7081 ctgtgcagag atctcctgaa ccaccggtcg cgcccatttg cttccaggaa gttgagttga
     7141 tccgggctca gagaaagagc aatcgaatca atttcgtctg cagcttcctg cactgttcga
     7201 attccttcca ctctgccgac gccgcggctc aagaatcgaa ctgaattgtt gatgcattca
     7261 atgttgtcgg cgtctggaac tgcagtgaga gttccatcgc ccatccattt cttaattcgc
     7321 acagaagtca tgcgatccca gtacgctaga tggcacagaa gtgtacctac agtccagtca
     7381 ccacccagat ccaatgacca gtgatggtct gtgagtcggg ccatgatggc cctcaggcga
     7441 tcgttttcac tcttgttgtc ctgggcgaag ctcatgtgcg gaaggaagca tttgatgagc
     7501 ttcagtcaag tttaattgtt cctttcgggc atgccttcgg ccaggtgact cacattcact
     7561 ctgccagttt ttccgcatcg ccaaatcgca cgatgtctgc cctggaagtt tttagccagt
     7621 ctgctgcgtg gatcgcaggc gaagatttct cctggtcaga atccagaatc ttgattccag
     7681 ccctggtgat ctttgcaaga gcgtctgcct cttctcccag tttgattttg aaatagtaat
     7741 aggttgcgag tcctgctgcg agttgcagac cttccgcgcc ttcccaggtt tttcccgatt
     7801 gttccccgag tgctttttcg ctttgaacga gaaagccatt gagtacgttt cgcacgcccg
     7861 tagaaaaagt aacatctttg agattcactt taccctgggc ctgatccatc actgcgattc
     7921 tagggatcat gctcaatccg tggccgctca cgtagactcc agtcagcgaa aaaggaccca
     7981 ctctctttcc gtaaaatgct tcgcagggaa tcaggattct tgagggtccg ctcccgcttt
     8041 gcacttcagg cgcattgtat ccgcttcgcg cgatttcaaa tggaagtggc tgcactgcgt
     8101 caccggatgc tttgaagaaa gtaagagtga ttgatcctcc gctttctccg cgatcaaacg
     8161 tttgcgcgta gagtagttct cctgatgttc cgcgcatggg ggctacgttc atgattctct
     8221 ggtgtccata atcccacgcc tgcaaacgga gcagggggcc tggatccttc tttttctgga
     8281 atactagaag atgtctgtca tcgcttccag tagaaaaaat tctgaagctt tgccactcca
     8341 gctgaaccgg agaagtttcc tccgcatgca gtcccagaca gacggcgaac gtaagtacaa
     8401 gcagtgttgt gcgaatcata aaatctctag ataggacgac ggcaggggct ctttccactt
     8461 ttttgttttt gaaattttta acaaaataga agcaagttcg ccgttgggca gatggctgat
     8521 gacgtcgtgg atgatggtct cgcgggccag atccggggcg atggctccgt tgagaagggg
     8581 aaagcgaaag cgcatcaaag aatcagggtc gattcccgca tcttcatcct cccagctatt
     8641 tgcagatatg atgccttttc cgttttccca gactgctatg ccgccgtcgt ttgaatgaaa
     8701 acgcagcgat ctcatgatca gctttgagtc atgaatcaga gtgagcgcac gcgaaatgaa
     8761 gtccttcttc aaataggacg cgaagacaac ttcaaagatg tcatcctgtg ccgttctgct
     8821 gatgaagctg agcgtctctt tgctcataaa tcattctccc accattccag gagattgtca
     8881 aagcggaatt ttgctttctg tctcagtcaa tgacacggat gcggatctgg ccgccgtctt
     8941 caatatctcg gatctgacgg aagaaatcca gttcaaagtc aatctgggca ttcgcttcca
     9001 ctgccttgag agacactccc tgtgccgatt cctttgtgca atagggtgga aacgcgtgga
     9061 tgagctgccc tggctcgagt ttctttccct ctttttcaag gcgaagcaga gggtgcattt
     9121 gcaagaactc caccgggtcc tcgtttgcac tggcaaagaa accgtccagg gaaagaccaa
     9181 gcggatcgag aaacgcggtc tcgagcgtga gcctgtacac cattccgtct ctgagtatga
     9241 actgatctcc catgcagtct t
//
LOCUS       DAORFF010000077         9141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3590_length_9141_cov_3.590579, whole genome shotgun sequence.
ACCESSION   DAORFF010000077 DAORFF010000000
VERSION     DAORFF010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3590_length_9141_cov_3.590579"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..255)
                     /locus_tag="PKK76_04795"
     CDS             complement(70..255)
                     /locus_tag="PKK76_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67816.1"
                     /translation="MSYMAVGSRQVLYSDTAPPDRGDVRALPVRTEPAQRTEDSISSP
                     RTDVRDQDRGQAVNTFA"
     gene            344..1846
                     /locus_tag="PKK76_04800"
     CDS             344..1846
                     /locus_tag="PKK76_04800"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL67817.1"
                     /translation="MSRLFPGLKVLSSMRLRIGLLYLALSVVNIVILTAMIFENQLDM
                     LRLNFRLQSDRIAGTVLTRLEELDPSRIDDLNTLDRVLNENHIISYVIFTERGDKLFE
                     KAKEASVLPATGQIEEPVRRSVLQLRPTALIRQRYTIELNESDYSAQYTMSLPGGRFE
                     QPGYIHAIMRIADFEARYRLLYRQAALAAGLVFLAHLIFALIAIRIFFRRIGLLAHAS
                     EHLAEGNLAARASWTMNRMDEVDLLGTTFNHMAQKIQSTVDALSGLNTEIQNELTIGK
                     EVQEQFLPDAKSLAKWNAAAISRPLREVSGDVYTFFQAGGQDGVFFADASGHGVSAAL
                     ITSLALLSLERIQETAYSAEEMALQMNAAIINHLKTSMFYMTGVFALFDGDRIHYVNG
                     GHPAPILIRKNGDIEILDPTGPPLGIKRDVSFEAGSIEASSGDRLFLYSDGLIESPSP
                     EGTVLDIEDVVNILMDHPDPTEAVNQIMVALDALLPVVRDDISILYLGVP"
     gene            1843..3258
                     /locus_tag="PKK76_04805"
     CDS             1843..3258
                     /locus_tag="PKK76_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL67818.1"
                     /translation="MKRGGIVLLFVSLALIWGLVFTSVWKVTGFLLRLDVRRQQLLNY
                     ELSSRLLAEKVRSTEEDFDREVEGHVLETTVLNFASLGSSPGLWETGGIALVNGVRLV
                     SGKGILHVSDDESTLRLLQYAFALERTKKLKEAEAVFETIRPSLKDENLAFALLHGGY
                     CKFLLGEDDKARDSLTKVVEQYPGTHFAASARTLLALLNTKREDGESAADSAYRLGRY
                     AMAAREYSSMKSLTDRQQFKLARSLEETGKMPAAVELYRKLASGADPVRKDSIRRLLI
                     LGTFLGGGEPVRSFATEKAKENSDPALQKIEEARTLAEAPKFLGRKNVTPEGIVAELK
                     RENPAIRIPEEEAREEPPKVESKEPPAEKRAQIAKPAPAAPTPIFRIPLIVRLSDGRA
                     ISCNQVRIHGSSARLIRQSATVETEVRSILSIESTDGYLTVNDSGIGTRRIDQEENFF
                     IVSGARRAYIPTSSLQIVEAR"
     gene            complement(3260..3832)
                     /locus_tag="PKK76_04810"
     CDS             complement(3260..3832)
                     /locus_tag="PKK76_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67819.1"
                     /translation="MSFTELDIDVEGNLNAQGKLPYTLQECRSVVKIPEVLKELVQKF
                     ILIQKSYGMALHDDSKMMGNERFTLRDLIDDCIDLVIVMRTKVETDETYAMSAPELGR
                     RAQFQIDLDRWKATGTLGANRNLKNNQFSIWLERMAKERLPAIIKYFGQISLDGVLTA
                     EEIAGFNKLLDRLFFSMVIVREEILSGEME"
     gene            complement(3832..5238)
                     /gene="sthA"
                     /locus_tag="PKK76_04815"
     CDS             complement(3832..5238)
                     /gene="sthA"
                     /locus_tag="PKK76_04815"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNL67820.1"
                     /translation="MDRFDLVCIGGGPAGQKAAIQAAKCGKRAALIDANERLGGASVH
                     TGTVPSKMLQETSRFLRRLRLEAGRGLSLEVPDKITMLDLLARNRVILHKEEDLAEDQ
                     AARNLVHVLRGRGRLTGPHTVEVTGPYGEKTSLESEYIFLSPGSRPNRPAGIPFEDQV
                     IYDSDGVLTLRKLPRRLAVLGAGIIGCEYASIFANLGVEVHLIDPAESILHWADRDIA
                     RTLSDSLSRIGMQIHTHDDVAEFRRTEDGVKIRTKKDVEFVVDQVLVSKGRQGNTEGL
                     GLESVGITASSRGTVKVSDVYQTEVPSIFAGGDIIGHPALSSTSMHQGMYVSRYLFLG
                     ESPRFARSQMPVAIWTIPEVAFIGATEEELKKAGVDYGVGKSDFSENTRALMTGESRG
                     LLKLIFDRPTKRLLAVHMVSDRATELLSLGQTAVHLGATVDYFFHHIFNYPTLSGVYK
                     SAALDAVNAYEMAPAAVR"
     gene            complement(5238..5522)
                     /locus_tag="PKK76_04820"
     CDS             complement(5238..5522)
                     /locus_tag="PKK76_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67821.1"
                     /translation="MKKISIAVFIAMSFLLPLCKSQSSQTTSKGDVVDCNYYWPARSR
                     YVCIKDMPRKECEELFAAGGFNREKTCVCDNGGKQKNMVEGTGYTQYDCK"
     gene            5529..6317
                     /locus_tag="PKK76_04825"
     CDS             5529..6317
                     /locus_tag="PKK76_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67822.1"
                     /translation="MMKAFWFSLQFFFLLAACKAYGPPFAGIAAGDRIPDSIAATTYA
                     ISIPQTFYRISQKAELTFERSGPTWTYFDLRIVGQNPIASIRVHSYEGKAFMTDGVLE
                     LRTEKCYERGKKDWEDRLTPLDGWDCEHLWFQLVELAPGSGIFQSARSDRTQYSDWIG
                     SITAVPLSRATFSGQILTTLPDQSVIVYGKNAGRLLRDGMTLEAREVDLLPLGRPAKK
                     DQTTGTLKVISRPGDFIVAQWEGTPAPANAAFSFAPRPAKSMFD"
     gene            complement(6333..7424)
                     /locus_tag="PKK76_04830"
     CDS             complement(6333..7424)
                     /locus_tag="PKK76_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67823.1"
                     /translation="MKPWLPLLLAVLYCAKPVEDPVPLVLIHGIKGSLLEDAEGQTAW
                     LSGREAMGWKHYRLSLPTEWKGEEQKRDGLRATGILKEVYVVPFLIGEKVYGPFIKGA
                     DKLDRPFYTFAYDWRRDNIENLRLFTAFLKEVSEKNRNAKVQVVAHSMGGLITRAALA
                     ENPELFHSVVFAGVPFSGGLGFLPDMHAGEPVGRNERILSPEVYFTFPSVYTLFQEGS
                     SRIYESGKELPINLYNADDWAKYKLGLFSLELVSPSQAAHLRMCLKRGKEFRRLASLP
                     VSKNIPVYVVLSKSTPTLATVERNGPKSVRGYDFESRPREPGDGRVREVDAFPPGLKA
                     ETLYIDKVHSELLNDTAVMDLVRKTGEKK"
     gene            7513..>9141
                     /locus_tag="PKK76_04835"
     CDS             7513..>9141
                     /locus_tag="PKK76_04835"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL67824.1"
                     /translation="MRIGLILAIVLLNSCGAPPGMRSLNSTQINWEAKPGIAQPEDEG
                     FRPVDVPGNAASGSKEITYRLKFDMQPGMDSYGIRLGEISDRDRTYLNGTLIGSTGDW
                     NSDRPQAYDRTRLYTIPSGVLKSGQNILLVQVHGYFDYELGIYRDRVEIGPMASLLRE
                     YYLDNAGQALSIMVYVTIAAYFLLFFFRRRQDRENLYFALFLLSFILYSFFRTQFKYE
                     FGWPLFITKRIQFLTLFASVPLFFLFITSYFPLPEKPWATWFNRIAWLVMLIPAGAAV
                     MVLASPSGIVWQATLDYVQPTWIFFILGILFVVLRETFIHKDKDAMIMIGAILILVAA
                     LVVDTLAGRGRINLPTLLTHAFTLFVIAIALVLANRFVRLHDETEELNANLASLNRAA
                     QRFVPFEFLNILEKRSLLEVNLGDQVQREMTVVFSDIRGFTTLSEKMTPKENFDFINS
                     YLRRVGPSVREHGGFIDKYIGDAIMALFPVSPGEAVKSCIQMLGTVDRWNEKRVEFKY
                     EAVHVGFGIHTGRLMLGTIGENERMEGTVISDDVN"
BASE COUNT         2345 a         2446 c         2176 g         2174 t
ORIGIN      
        1 gtaagaatgg aaacaacgaa taaggctcta tacattagaa atctccggat ttgaaacgtt
       61 atacgacatt caggcaaaag tattcacggc ctggccgcga tcctggtccc gcacatctgt
      121 gcggggagaa gaaatagaat cctctgttct ctgcgcaggc tctgtcctca caggcaaagc
      181 ccgcacatcc cctctgtcag gaggagctgt gtctgagtaa agcacctggc gtgacccgac
      241 agccatgtag gacatgttaa gattcttaag ccccccggcc gaaaggtcaa gtctttcccg
      301 attaacgatt gcactccaga ccggcccgcg caccttcttc gcaatgagca gactcttccc
      361 gggactcaaa gtcctcagct ccatgcgcct gcgcattgga ctattgtatc tggctctatc
      421 ggttgtaaac attgtcattt taactgccat gatctttgaa aaccagctgg atatgctgcg
      481 tttgaatttc aggcttcagt cagatcgaat tgcgggtacc gtgcttacaa gattggaaga
      541 actcgatcca tctagaatcg acgacttaaa tacactcgat cgcgtgctca atgaaaacca
      601 tatcatatcg tatgtgatct ttacagaacg cggagataaa cttttcgaaa aagcaaagga
      661 agcttccgtg cttccagcga caggccagat agaagaaccg gtgcgaagaa gcgtgctaca
      721 attgcgaccc actgcactca tacgacagag atacacaatc gaactcaatg aatcagacta
      781 ctcagcacaa tacacgatga gccttcctgg cggaagattt gagcaacctg gatacatcca
      841 tgcaatcatg cgcatcgcgg actttgaagc aaggtacaga cttctctaca gacaggccgc
      901 cctcgcggca gggctggttt ttctcgcgca cttgatcttc gcactgatcg ccattcgaat
      961 cttctttcgc aggatcgggc tacttgcgca tgcgagcgaa catctggctg aagggaatct
     1021 cgccgcgcgc gcatcctgga ccatgaacag aatggacgaa gtagatttgc tcggaaccac
     1081 gttcaaccac atggcgcaga aaattcagtc aacagtcgac gctctctccg ggctcaacac
     1141 tgaaatacaa aacgaactca ccattggcaa agaagtgcag gaacagtttc ttccggatgc
     1201 aaagagtctt gccaaatgga atgcggccgc tatttccagg ccccttcgtg aagtcagcgg
     1261 agatgtgtac acgttctttc aggcgggtgg acaagatggg gtattttttg cggacgcctc
     1321 cgggcatgga gtatctgcgg ctctcatcac aagtctggcg cttttgagtc tcgaaagaat
     1381 ccaggaaacc gcctattccg cggaagaaat ggcactgcaa atgaacgctg ccatcatcaa
     1441 ccacttaaaa acgtcgatgt tttacatgac gggggtcttc gcactgttcg acggagatcg
     1501 cattcactat gtaaacggag gccatcctgc gcccatattg attcgcaaaa acggggacat
     1561 cgagattcta gatccaaccg gaccgcccct cggaatcaag cgagacgtat cttttgaagc
     1621 tggatccata gaggcgtcga gtggtgatag actgttcctt tactcagacg gactcatcga
     1681 aagcccttcc ccggaaggca ctgtgctcga catcgaagat gtggtaaaca ttctcatgga
     1741 tcatccagac ccgacagaag cagtcaatca gatcatggta gctctcgatg cgctccttcc
     1801 agtcgtaaga gacgacatta gcatcctgta tctgggagtg ccatgaagcg cggcgggatt
     1861 gttctcttgt tcgtttcgct cgcgttgatc tggggtctcg tttttacttc tgtctggaaa
     1921 gtgactggat ttctgcttcg cctggacgtt aggcgacaac aattgctgaa ctacgaactt
     1981 tcctctcgct tgctcgcaga aaaggtacgc tctaccgagg aagactttga tcgtgaagta
     2041 gaagggcatg tgctggaaac aacggtgcta aatttcgcca gccttggaag ctctcctggt
     2101 ctatgggaga cgggcggaat tgcactggtc aacggagtac gccttgtctc aggcaaagga
     2161 attctccatg tgtcagatga tgaatccact ctccgactcc tccagtacgc ttttgcgctc
     2221 gaaagaacaa aaaagctaaa ggaagcagag gcagtatttg aaaccatccg cccttccctc
     2281 aaagatgaga atctcgcctt tgcgttgtta cacggtggtt actgcaaatt tctgcttgga
     2341 gaggacgaca aagcacgaga ttctttaaca aaagtagtag agcaataccc cggaactcat
     2401 ttcgcggcat ctgcacgaac tctactcgcc cttctcaata caaagagaga agacggagaa
     2461 tcagctgcgg acagcgcgta tcgcctgggg cgctatgcaa tggctgctcg agagtacagt
     2521 tccatgaaat cacttacaga caggcagcaa ttcaagctgg cccggtccct cgaagagacg
     2581 ggaaagatgc cggcggcggt agaactctat cgaaagcttg cttcgggcgc agatccggtg
     2641 cgaaaagatt ccataaggag actgctgatt ctgggaacat tcctgggcgg aggagaaccc
     2701 gttcgcagct ttgcgacaga gaaagcgaag gaaaacagtg accccgcttt gcagaagata
     2761 gaagaagctc gcacactggc agaagctccc aaattccttg ggcggaagaa tgtgactccg
     2821 gaaggaattg tagcggagct caagcgagaa aaccctgcca ttcgaattcc agaggaagaa
     2881 gcgagagaag aaccgccaaa agtggaaagc aaagaacctc cggcagaaaa acgagcacaa
     2941 atcgcaaaac cggcacctgc cgcacccact cccattttca gaattcctct cattgtgcga
     3001 ttgagcgacg gacgcgcgat ctcatgcaat caggtgcgca tccatggttc gagcgccagg
     3061 ctgattcgac aatcggctac tgtggaaaca gaagtacgat ccatcctcag cattgaatct
     3121 accgacggat atctaacggt aaatgattcg gggattggta caagaagaat tgatcaagaa
     3181 gagaacttct tcattgtttc cggcgcgcgg cgagcataca ttccaacttc ctcgctgcaa
     3241 attgtagaag ccaggtaact tattccattt ctccgctcag tatttcttcg cgcacaatga
     3301 ccatgctaaa aaacaatcga tcgagcagct tgttgaatcc ggcgatttcc tctgcagtca
     3361 aaacaccatc cagggaaatc tgtccaaaat acttgatgat ggctggcagg cgttcctttg
     3421 ccattctctc aagccagata gagaactgat tgtttttcaa gttgcgattg gcaccaagag
     3481 tgcctgttgc cttccatcga tccagatcta tctgaaactg ggcgcggcgt ccgagctcag
     3541 gcgcggacat ggcatacgtt tcatccgttt ccaccttcgt gcgcatgaca atgacaagat
     3601 caatgcaatc atcaatgaga tcgcgcagag taaaacgttc atttcccatc atcttgctgt
     3661 cgtcgtgaag agccattccg tagcttttct gaatcaagat gaatttctgg accagctcct
     3721 tgagcacttc cgggatcttc acaacagacc tgcattcctg caaagtatac ggaagcttcc
     3781 cctgagcgtt tagattgcct tctacatcaa tgtccagttc cgtaaaagac atcaacgcac
     3841 cgccgccggc gccatttcat acgcattcac agcatcgagt gcggcactct tataaacgcc
     3901 gcttaacgtt ggatagttga atatatgatg gaaaaaataa tccacagttg cccccagatg
     3961 aactgcggtc tgcccgaggc tcaagagctc tgtcgcacgg tccgaaacca tatgcactgc
     4021 cagaagcctc ttcgttggcc gatcaaaaat caatttcaaa agacccctgc tctccccggt
     4081 catgagagca cgcgtattct cgctgaagtc tgatttgccg actccataat ctacaccggc
     4141 ttttttcaat tcctcttctg tggcaccaat gaatgctact tccggaatcg tccagatggc
     4201 aacgggcatt tgcgaacgag caaaccttgg actttctccc aaaaacaggt atctggaaac
     4261 atacattccc tgatgcatgc tggtgctaga aagagcagga tgaccgatga tgtcgccgcc
     4321 cgcgaaaata gaaggaactt ctgtctggta aacgtcgctc accttaacgg ttcctcgaga
     4381 agaagctgtg ataccaacag attcaagacc caggccttcc gtatttccct gtctaccttt
     4441 ggaaaccagc acctgatcca ccacgaattc cacatctttc tttgtgcgaa tctttacgcc
     4501 gtcttccgtg cgacgaaact cagctacatc gtcgtgagtg tggatttgca ttccaattct
     4561 cgaaagagaa tcactcagtg ttcgcgcaat gtccctgtct gcccagtgta gaatggattc
     4621 tgctggatcg atcaggtgaa cttccactcc aaggtttgca aaaatacttg catattcaca
     4681 accaatgatg ccggccccga gtactgcaag acgacgcggc aatttccgca gagtcagcac
     4741 tccgtctgaa tcataaatca cctgatcttc aaatggaatt ccagcagggc gattcggacg
     4801 tgagccagga gataaaaata tgtattctga ttcgagagaa gttttctctc catatggtcc
     4861 ggtcacttct acggtgtgcg gccccgtcag gcgaccacga ccgcgcaaaa catgaaccag
     4921 atttctggca gcctgatcct ctgccaggtc ctcttctttg tggagaatga cacgatttct
     4981 ggccagaagg tccagcatcg tgatcttatc aggaacttcc agggaaaggc cgcggcccgc
     5041 ttccaggcga agcctccgaa gaaacctgct ggtttcctgc agcatcttgg aaggcaccgt
     5101 gccggtgtga accgaggcac ctcccaggcg ctcatttgca tcaatcaaag cggcgcgttt
     5161 gccgcacttg gctgcctgaa tggcggcctt ctgccccgca ggtccgccgc caatacaaac
     5221 caggtcaaaa cgatccatta tttgcagtcg tactgagtgt agccagtgcc ctccaccata
     5281 ttcttttgct ttccaccgtt gtcgcaaacg caagtttttt cacgattgaa tccgccagca
     5341 gcaaagagct cttcgcattc tttacgaggc atgtctttga tacaaacata gcgagaacgg
     5401 gcgggccagt agtagttgca gtccacgaca tctcccttgc tggttgtctg agacgattga
     5461 cttttgcaga gtggcagaag gaatgacatt gcaatgaaca cggcaatact gatttttttc
     5521 atgagtgtat gatgaaagcc ttctggttca gcctacaatt ctttttttta ctggccgcgt
     5581 gtaaggcgta cggaccgccc ttcgccggga tcgccgctgg agacagaatc ccggattcga
     5641 ttgccgccac cacgtacgcg atttcgatcc cgcaaacgtt ttatcgcatt tctcagaagg
     5701 ctgaactgac ctttgaacga tctggcccaa cctggaccta cttcgatctt cgcattgtgg
     5761 ggcaaaatcc gatcgcctcc atcagagttc attcctacga aggcaaagca ttcatgacgg
     5821 acggggtact cgaacttcgc acggagaaat gctacgaacg cgggaaaaaa gactgggaag
     5881 atagactcac tcccctcgat ggctgggatt gcgaacacct ctggttccag ctagtggagc
     5941 tggcaccagg atccggaatt tttcaaagcg cgcgatccga tcgcactcaa tactcagatt
     6001 ggattggaag cataacagca gttccacttt cgcgggccac tttttccggc cagatattga
     6061 ccactctccc cgaccagagc gtgatcgttt atgggaaaaa tgcagggaga cttctgcgcg
     6121 atggtatgac cctggaagca agagaagtgg atttgcttcc gctcggaaga ccggcgaaaa
     6181 aggatcagac taccggaacc cttaaggtaa tctcaagacc cggcgacttc attgtagcgc
     6241 aatgggaagg gacacctgcc ccggccaatg cagctttcag ctttgctccg cggccggcaa
     6301 agagcatgtt cgactaacaa tcgttatgcg atctacttct tttcgcccgt ttttcgaacc
     6361 agatccatga cggccgtatc gttcaatagt tccgagtgca ctttgtcgat gtagagcgtt
     6421 tctgctttca aacctggtgg aaacgcatcc acttcacgca ctctcccgtc tcctggttcg
     6481 cgcggtctcg attcaaaatc atagccgcgc acactcttag gaccatttct ctcgactgtg
     6541 gccagagtgg gcgtagattt agagagaacc acatacacgg gaatattttt ggacacaggc
     6601 aaactggcca ggcgacgaaa ctctttgcct ctcttcaagc acatccggag gtgagctgcc
     6661 tgtgaagggc tcacgagttc gagcgaaaaa agcccgagct tatactttgc ccagtcatcc
     6721 gcattgtaga gattgatcgg aagctctttt ccggattcgt aaatccgcga tgagccttcc
     6781 tggaacaggg tatacacaga aggaaaggta aagtacactt ccggagataa gatgcgctca
     6841 tttctgccga ccggttctcc cgcatgcatg tccggtagaa atccaaggcc ccccgaaaaa
     6901 ggaactccag cgaaaacgac ggaatgaaac aactcaggat tttctgcgag tgccgccctt
     6961 gtgatgagac cacccatgct atgagctacc acctgcacct tcgcattccg attcttttca
     7021 ctcacctctt ttaagaaagc agtgaaaagc cgtaggtttt ctatgttgtc gcgtcgccag
     7081 tcgtaggcga acgtataaaa cggacgatcg agcttatctg cacctttgat aaaaggaccg
     7141 tagacttttt caccgatcag gaaaggaacc acatacactt ctttgagtat acccgtggcc
     7201 cggaggccgt ctctcttctg ctcttctcct ttccattcag tcggcaaaga aagacgatag
     7261 tgcttccacc ccatcgcctc acgaccgctt aaccaggctg tttggccctc cgcgtcctct
     7321 agaagagaac ccttgatccc atgaatcaac acaagaggca caggatcctc cactggtttt
     7381 gcacaatata gaacagctaa aagtaaggga agccacggct tcatgcggta atctggttgt
     7441 ctcaggttcc cgattcaagt ccttttcgac aaaaaggatt gacccttccg cgccgccgga
     7501 ccatcctctg gcgtgcgaat tggattgatc ctggcaatcg tcctcctgaa ttcttgcggt
     7561 gctccgcctg gaatgcgaag cctcaattcc actcagatca actgggaagc aaaaccagga
     7621 atcgcacagc cagaagacga aggctttcgc ccggttgacg ttccgggcaa tgcagccagt
     7681 ggctccaaag aaatcacata tcgactcaaa ttcgacatgc agcctgggat ggattcctat
     7741 ggcatccgcc tgggagaaat ctcagaccgc gacaggacct atctcaacgg gaccttaatc
     7801 ggtagcaccg gagattggaa ttccgacagg ccacaggcat acgatcgcac acgtctctac
     7861 accatccctt ccggtgtact gaaaagcggc cagaacatac tccttgtgca agtgcacgga
     7921 tatttcgact acgagctcgg tatctataga gacagagtgg aaatcggacc catggcttcc
     7981 ctccttcgag agtattacct ggacaatgca ggccaggctc tatccatcat ggtttatgtg
     8041 acgatcgccg cgtattttct cctcttcttc ttcagaaggc gccaggatag agaaaatctt
     8101 tatttcgcat tgtttctttt atctttcatt ctttacagtt tctttcgcac acaattcaaa
     8161 tacgaattcg gctggcctct ttttatcaca aaaagaatcc agtttttaac gttattcgcc
     8221 tctgttccgc tcttcttcct ctttatcacg tcctatttcc ctcttccgga aaagccctgg
     8281 gccacatggt ttaatagaat cgcctggctg gtcatgctca ttcccgcagg cgcagcagtc
     8341 atggttctcg catcgccctc aggcatagtc tggcaagcca ctctagatta cgtacagcca
     8401 acgtggattt tcttcatact tggaatcctc ttcgttgtcc tcagggaaac cttcattcat
     8461 aaagacaaag acgctatgat catgatcggc gcgattctga ttctagtggc agcactcgta
     8521 gttgacaccc tcgccgggcg cggccgcatc aacttaccca ctctgctcac tcacgctttc
     8581 acgttattcg tcattgctat tgcactggtt cttgcaaacc gatttgttcg cctccacgac
     8641 gaaacagaag aactcaacgc aaatctggca agcctcaacc gggcagcaca aagatttgtt
     8701 ccttttgaat tcctgaacat cctggaaaag agaagcctcc tggaagtaaa cctgggggac
     8761 caggtgcaaa gagaaatgac tgtggttttc tctgacattc gcgggttcac gactctctca
     8821 gagaagatga cccccaagga gaactttgat ttcatcaact cgtatctgcg ccgcgtaggc
     8881 cccagcgtgc gcgaacatgg cggtttcata gataagtaca ttggtgacgc catcatggca
     8941 cttttcccgg tcagcccggg agaagccgta aagagctgca tacagatgct cggcactgta
     9001 gaccgctgga acgaaaagcg agtcgaattt aagtatgaag cggtgcacgt tggattcggg
     9061 atccataccg gaagactgat gctcggcacc atcggcgaaa acgaacgtat ggaaggaaca
     9121 gtgatttccg acgatgtgaa t
//
LOCUS       DAORFF010000078         9083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3612_length_9083_cov_3.928666, whole genome shotgun sequence.
ACCESSION   DAORFF010000078 DAORFF010000000
VERSION     DAORFF010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3612_length_9083_cov_3.928666"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PKK76_04840"
     CDS             <1..421
                     /locus_tag="PKK76_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNL67825.1"
                     /translation="VFRNEDIDSPEHLEDIILASSSVPPMVKFQKVKGRYYLDGGITR
                     NLPVALLENVDVAIGIYYNFWTRLQYEGVNVVDGGQEIEKMEKGKKAGPRVILVSPER
                     DLPIQTWDYANPRGIELAYEAGLRAGEHLLPIVKKMR"
     gene            519..3737
                     /locus_tag="PKK76_04845"
     CDS             519..3737
                     /locus_tag="PKK76_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNL67826.1"
                     /translation="MSIASVSIKRPIFITSIVLVIIFTGAFSLLKLGVDLFPDVNFPF
                     VVVSTVYTGAGPEEVENLISRPLEEELSSLQGVKRITSRSQEGFAVVFVEFTLSTDIK
                     YGEQQVRNRVSRVRRLLPSSIEEPIIVRFDPADQPVVRYALFADLPPARLYELAKEKI
                     KPRLEQAPDVGSIALIGGVKREIQIEIDRNKLNALQLSAVGIGQSMKNYGLNVPVGKH
                     EQGASETTFRTIGQFDSLKQIEEVGISFGGEVGNAVSLRSVATVRDGTEDAKNICELW
                     APTTTQPEYGFFSRNRHAQIERQNNAALFIDIYKQSGSNTVTVTDGVKLRVEKLNEFL
                     KDQEGHPRLVMVRDGSRWIRANVEDVGLAIVLGILLTVLVVYLFLGNIRSTIITGLAL
                     PNSLLGAFILMYIMGFTINVMTLLALSLAIGLLVDDAIVVRENIFRKMELGESPVKAA
                     EDGTTEVALAVIATTATVLAVFLPIGFLSGIVGQFFKQFGLTVCFAMIISLFDAMTIA
                     PMLSAYFGGQVHGEPNFVVRGFRRFQDFLEKGYIAIMRFGLKRPIVIILLTVLVFVGS
                     ILTLGGVKKTFLPPNDQGEFMVSIQLPPGTALQGTYDAALKIREKLQKIPEMNLIAMV
                     VGSTEGEPNVAIFGVGLTDFTQRTRSTQKVQEEIREMLKPYAEWKPSVNEYSAVGGGV
                     QYPFNVNIMGDDLQQLQPYAEQVVTMLKGIPDLTDVSMDYRAGKPEYRIEFDPNRMQN
                     VGVNPTVAGAELRYHIAGEVVGTLRERGYEYDIRMRLRPEQRNLKSAYAQTRVPNNQF
                     KMIPLAAIATGRPALGPEKITRQDRSRVIQIHANLAPGGAIGSATDIAKKALTTKLIP
                     PKGTSYVFWGQSEDFIELLVNVGIAFLFAIVFIYLVLASLYESFITPLAILISIPPAI
                     SGAFLSLYIFNEMLNIFSMIGLILLMGLVTKNSILLVDYALEGIRGGLDINEAILRAG
                     RTRLRPILMTSLAMIAGTMPIALGLGEASRARTAMGIAIIGGIVVSTVLTLIVVPAIF
                     GYIERFREWIEAKFRPAEIEAIRLQELSSMSQAMREPEKKRK"
     gene            complement(3830..4654)
                     /locus_tag="PKK76_04850"
     CDS             complement(3830..4654)
                     /locus_tag="PKK76_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67827.1"
                     /translation="MSIGNLPVLAHDMVLMIKAEVKRDLLYMKRYPLEIISFMFFMFL
                     ILIAIIYGIDFLLPKGSGISDKGNKEKLVLGYCLMQFVMATQMGWSGQIQNESQTGTL
                     EQLSISGHSLGGVLFARGIAQLPRQTITFYIMLALYRIALPGSAISLNHMLAGSLDLL
                     IMAIGIFGISYIFAGITLLFKRVGFFFQIINFAFLGLFWIDRAKLPEILQNIYDHFPL
                     TVGMSRLMQTLTDPVIASPYSMIEFIVYSFLFMAVGWFCFSRMERTARSYGLLSQY"
     gene            complement(4651..5604)
                     /locus_tag="PKK76_04855"
     CDS             complement(4651..5604)
                     /locus_tag="PKK76_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNL67828.1"
                     /translation="MSLQVVNLKKTYKNPLSRKPPFEAVRGVSFEIKPGEIYALIGPN
                     GAGKSTIIKMIAGLIVPSSGDVMLDGKKLGSETAAYKKLSAVLEGTRNVYWRLTPLEN
                     LHYFANLRGISTTEIREKSVQLLKTLEIEGKKKNQSQHLSRGMLQKLALGAALITDPQ
                     IILLDEPTLGLDVGSSRKIKEMMLELTRKHGKSILLTTHQMDLVEEVADRAGIIREGK
                     IVREGTIPELRNVFRNHIYRFSVRGKFKLPPEWQKKYGAALLSGQNDHTEWEMDCKKP
                     EGLYTVLSALGKKKLEIISMHKPVEDLEEIFMRTLSEEATR"
     gene            complement(5601..7418)
                     /locus_tag="PKK76_04860"
     CDS             complement(5601..7418)
                     /locus_tag="PKK76_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNL67829.1"
                     /translation="MAALIQISGLHKQYGPRVLFDDAEVVISEDQKFGVIGRNGAGKS
                     TLFNIITSKEEADSGSVWLSSDLRLSYLEQKDTHLPDETVIDFLMRTTDQEEWRCGKY
                     AGRFQIKGDMLGAKISGLAGGFRMRVKLTAMMLEEPNLLMLDEPTNYLDLKTLLMLEH
                     FLRTFRGAALIISHDREFLMKTCKHTLEVDNKTIASFPGNVEEYLEYKREVREQAIRE
                     NQNIEARRKELQNFVDRFRAKASKATQAQSKMKQISRLKTKEVAGAARSVRMRIPSPD
                     RQGGVALKCKDLRIGYGDRMIADNISFELKRGDRFAILGDNGQGKSTFLKTIQGSLPA
                     LAGEVRWGYDTKIGYYAQHVYETLGGEETVLQYLERYAPKSMTRQEILNMAGAFLFGG
                     DDTKKKVSILSGGERSRVLLLSLLLQKCDILLLDEPTNHLDFDTVEVLGEALQDFKGT
                     VFFVSHDRTFVNGVATGILEVKNKAIRLYEGSYEDYVYYLNEELDRAEASDDSEPVEQ
                     VETVQAAPPPGGKAKADKKARDKELKALEKKHATLGRELETLQQEQKDLLALVEKNPD
                     TVSKADYDRIGQLSSSVEEKEAEWLKVAESLERAKEPAG"
     gene            7498..8037
                     /locus_tag="PKK76_04865"
     CDS             7498..8037
                     /locus_tag="PKK76_04865"
                     /inference="COORDINATES: protein motif:HMM:NF016819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HNL67830.1"
                     /translation="MRVLIALLFLVPAGLFAHPGHAAGPLSGLWHPFTGLDHLLVMVA
                     VGFVASRLGGKVRLFAPAAFLLAMISGASASWAGVSLPGVEPLVQLSLAVFGVLLMAG
                     FRMHSGFFLVLVGVAGFFHGAAHASEGPAAISYAIGFLASTAALHVAGLLLGNIQTRA
                     GSVLSRAVGAAMIALAMAG"
     gene            complement(8047..9042)
                     /locus_tag="PKK76_04870"
     CDS             complement(8047..9042)
                     /locus_tag="PKK76_04870"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNL67831.1"
                     /translation="MKTTVFGVGAWGTTLAGILAENGHDVVMWSHDPEIARTINEEHE
                     NKALFAGVKLSDKIRATTDLKEACVDSKIVVFVVASQFYASTVQKAHPFLPRDVVIVS
                     ATKGLNPADNRRPSEVLIENLPPEMKTRVAVLSGPNIAREIALKKPATTVIASPNEEI
                     AKAAQKFFSTPYFRVYTNTDMAASELGGTLKNVIAIAAGIVDGLDLGDNTKSALMVRG
                     LVEIIRFGTHFGAKADSFYGLAGMGDLITTCSSKMSRNHFVGESLAQGKTLKDILAGM
                     TAVAEGVEAARHVHEIAQREKIEMPVTTQVYKVLFEDKPVKQAIMDLMTRDLKAE"
BASE COUNT         2016 a         2235 c         2321 g         2511 t
ORIGIN      
        1 agtctttaga aatgaagaca ttgattcgcc agagcatcta gaagacatca tccttgcatc
       61 gagcagcgtg ccgccgatgg tgaaatttca gaaggtgaaa ggccgctact atctagatgg
      121 gggaattaca cgcaatttgc ctgttgcatt gctcgaaaac gtggatgtgg cgattggcat
      181 ttattacaac ttctggaccc gcctgcaata cgaaggagtc aacgttgtgg acggcggcca
      241 ggaaatagag aagatggaaa agggcaagaa ggccggtccg agagtgatcc ttgtatcccc
      301 ggagcgcgac ctccctatcc agacctggga ctatgcgaac ccccgcggaa ttgagctggc
      361 gtatgaggcc ggactgaggg ccggcgagca tcttttgccg atcgttaaaa aaatgcgctg
      421 agcaaagccc gcgccgttct gcttcaaaat cctgtctctg ccgcgatcat tcggaaaaaa
      481 aaacagacag cctggtccat ttcccgagac tcgccctcat gtcgatcgca agtgtctcga
      541 tcaaacgccc catttttatc accagtattg ttctggtcat catcttcaca ggtgcattct
      601 ccttattgaa gttaggcgta gatttgttcc cggatgtgaa cttcccgttt gtggttgtga
      661 gcaccgtgta cacaggcgcc ggtccggaag aagtagagaa tctgatctct cgccctctgg
      721 aagaagaact cagttccctt cagggtgtga agcgaatcac ttcgcgcagc caggaaggct
      781 tcgcagttgt gtttgtagag ttcactctca gtacagacat caagtatgga gagcagcagg
      841 ttcgaaatcg agtctcgcgc gtgcgacgcc ttttgccaag ttccattgaa gagccaatca
      901 ttgtgcgatt tgatccggca gatcagcctg tggtccgtta tgcgctcttc gcagatcttc
      961 ctccagctcg cctttatgaa ctggcaaaag aaaaaatcaa gccaaggctg gagcaggcgc
     1021 cggacgtggg atcaatcgca ctcattggcg gagtgaagcg cgaaattcaa attgaaattg
     1081 atcgcaacaa actcaacgca ctgcagcttt ccgcagttgg aatcgggcaa tccatgaaga
     1141 attatggcct gaatgttccc gtgggtaaac atgaacaggg tgcatctgaa accacattca
     1201 ggacgatcgg tcagtttgat tcgcttaaac agattgaaga ggtgggcatt tcatttggtg
     1261 gagaagtggg caatgctgtc agtctccgaa gcgtggcaac tgtaagagat ggaacagaag
     1321 atgcgaagaa tatttgcgaa ctctgggcgc ctaccaccac acagccagag tacggattct
     1381 tttcgcgcaa tcgacatgcg cagatagaac gccagaacaa tgctgctcta ttcattgata
     1441 tttataaaca atccggttcc aacactgtta cagttacgga tggcgtgaaa ctccgggtgg
     1501 agaagctcaa cgaatttcta aaagatcagg aagggcatcc aagactcgtc atggttcgtg
     1561 acggttcgcg ctggatccgc gcaaacgtgg aagacgtggg cctggcgatt gtgctcggga
     1621 ttcttttgac tgtgctcgtt gtttatctgt tcctgggtaa cattcgttct acgatcatta
     1681 ccggtctggc acttccaaac agtttgctcg gtgcattcat tttgatgtac atcatgggct
     1741 ttaccataaa cgtgatgacc cttcttgctc tgtctcttgc gattggtctg ctcgtagatg
     1801 atgcgatcgt agtgcgtgaa aacattttca gaaagatgga gctcggagaa tctccggtca
     1861 aggccgcgga agatggaacg actgaggtgg ctcttgctgt gatcgcgact acggccacag
     1921 tcctggcggt gttcttgccg atcggctttc tctccggcat cgttggtcaa ttttttaaac
     1981 aatttggtct gactgtttgt tttgcgatga tcatttctct ctttgatgcg atgaccattg
     2041 cgcccatgct ttctgcttat tttggcggcc aggttcatgg agagccaaat ttcgttgtga
     2101 gaggtttccg gcgttttcag gactttcttg agaaagggta cattgcaatc atgcgatttg
     2161 ggctcaagcg tcccatcgtc attattctgc tcactgtgct cgttttcgtt ggaagtattt
     2221 tgactctggg tggcgtaaaa aagaccttcc ttccgcccaa tgatcagggc gaattcatgg
     2281 tctctatcca acttcctccg ggcacagcac tgcagggaac ttatgatgct gctctcaaga
     2341 ttcgcgaaaa gcttcagaag attcctgaaa tgaatctcat tgcaatggtc gtcggaagta
     2401 cggaagggga gccaaacgtt gcgatctttg gcgtaggttt gaccgacttt acgcaacgca
     2461 ctcgaagcac gcaaaaggtt caggaagaaa ttcgcgaaat gctcaaacca tatgctgaat
     2521 ggaaaccttc cgtgaatgaa tacagtgctg ttggcggcgg cgttcagtat cctttcaacg
     2581 taaacatcat gggagacgac ttgcaacaac tgcagcctta cgcagagcag gtagtgacca
     2641 tgctcaaagg aattccggat cttacggacg tctccatgga ctatcgggcc ggtaaaccgg
     2701 aatatcgcat tgagtttgat ccaaaccgca tgcaaaacgt gggagtcaac ccgactgtgg
     2761 ccggggcaga gcttcgctat cacatcgcag gtgaggtagt gggaacgctg cgcgaacgcg
     2821 gatatgaata tgacattcgc atgcgccttc gtccggagca aagaaacttg aaatccgctt
     2881 acgcgcaaac gcgtgttcct aacaatcaat tcaagatgat tcctctggcg gccatcgcca
     2941 caggaaggcc ggcccttggt ccagaaaaga tcacgcgtca ggatagatct cgcgtgattc
     3001 agattcatgc aaacctggct ccgggaggtg cgattggatc tgcgacagac attgcaaaaa
     3061 aagcgctcac tacaaaactg attccgccca aaggaaccag ctacgttttc tggggccaat
     3121 cagaggactt cattgaactc ctggtgaatg tgggcatcgc tttcttattt gcgattgtat
     3181 tcatttatct ggttcttgcg agtttgtatg aatcgttcat tactcctctt gcgattttga
     3241 tttcgattcc acctgcgatt tctggtgcgt tcctttccct ctacatcttc aatgagatgc
     3301 tcaacatctt ctcgatgatt ggtttgattc tattgatggg ccttgtgacc aagaactcga
     3361 ttctgctggt ggactacgct cttgaaggaa tcaggggtgg cctggacatc aatgaagcga
     3421 ttctcagagc gggtcgaacc agacttcgac ccatcttgat gacgagcctt gcaatgatcg
     3481 caggcaccat gccgattgcg ctcggtctcg gggaagcgtc tagagctcgc actgcgatgg
     3541 ggattgccat catcggtggt attgttgttt ccacagtgct gactctcatt gtggttcctg
     3601 caatcttcgg atacattgag agattccgcg aatggatcga agcgaaattc cgccccgcgg
     3661 aaattgaagc aattcgactt caggaactca gctctatgtc ccaggcaatg cgcgagcctg
     3721 agaagaaacg caagtaagcc ttcgcgaagc tcacgaagaa ttgcgggcag gtgcgtccgg
     3781 atttccgggc gcgacctgtg atgtgggcgt ttcttactct gagattggat cagtactgag
     3841 agagtagtcc gtaactgcgt gccgttcttt ccatgcgaga aaagcaaaac cagcccactg
     3901 ccataaacaa gaacgaatac actatgaatt caatcataga gtagggggac gcgatgactg
     3961 gatcggtgag tgtttgcatg agcctggaca ttccaactgt gaggggaaag tgatcgtaaa
     4021 tgttctggag aatttcagga agcttggccc ggtctatcca gaacagaccc agaaaagcaa
     4081 aattgatgat ctgaaagaag aacccgactc ttttaaaaag tagagtgatc ccggcaaata
     4141 tataagaaat gccaaatata ccgatggcca tgatgagaag atccagagaa ccagccagca
     4201 tgtggttcaa ggatatagcg cttccaggaa gagcaatgcg ataaagagca agcatgatgt
     4261 agaacgtaat ggtctgtctt ggcaattgtg cgattccgcg cgcgaataga actccgccca
     4321 gagaatgacc tgatatagaa agctgttcga gcgtgccagt ctggctttcg ttttgaatct
     4381 ggccggacca tcccatttga gtggccatta cgaattgcat gaggcaataa cccagtacga
     4441 gcttttcttt gttccctttg tcgctgatgc cggatccctt tggcaaaaga aaatcgattc
     4501 cataaatgat cgcgatgagg atcaaaaaca taaagaacat aaacgatatg atctcaaggg
     4561 gatatcgctt catgtagagc aggtctcttt tgacctccgc cttgatcatc aagaccatgt
     4621 cgtgtgccaa tacggggaga ttgccaatgc tcatctggtt gcctcttcgc tcagtgtgcg
     4681 cataaaaatt tcttccaggt cttctaccgg cttgtgcatc gaaatgattt cgagtttctt
     4741 ttttccaagc gcggacagaa ctgtgtagag accttccggc ttcttgcagt ccatttccca
     4801 ttcagtgtga tcgttctggc cggacaggag tgctgcgccg tacttcttct gccattcagg
     4861 cggcagtttg aattttcctc gaacgctgaa tctgtagatg tgattgcgaa atacatttct
     4921 aagttcaggg atggttcctt ctcttacaat cttgccttcg cgaatgatcc cggcacggtc
     4981 tgccacttct tctactagat ccatctggtg tgtggtcagt agaatggact tgccgtgctt
     5041 tcgcgtaagt tctagcatca tttctttgat cttgcgcgag cttcctacat ccaggcccag
     5101 agttggttca tcgagtagaa tgatctgagg atcagtgatc agtgctgctc ccagagcaag
     5161 tttctgaagc attccgcgcg aaaggtgctg gctttgattc ttctttttgc cttcaatctc
     5221 tagagtcttg agtaactgaa ctgacttttc tctaatttcc gtggtcgaga ttccgcgcag
     5281 gttcgcgaag tagtgcaggt tttcgagtgg agtgagcctc cagtacacgt ttcgcgtacc
     5341 ttcgaggact gcgctcagct tcttatatgc ggcagtttcg cttcccagct tttttccatc
     5401 gagcatcacg tccccggaag aaggcacaat caaaccagcg atcatcttga tgatcgtgga
     5461 tttgccggct ccgtttggcc cgatgagtgc gtatatttct ccgggtttga tttcgaagct
     5521 gactccgcgg actgcttcaa agggtggttt tctggaaagt gggtttttgt atgttttctt
     5581 taggtttact acctgcaggc tcatccggcc ggctcctttg ccctttccag ggattctgcg
     5641 accttgagcc actcagcctc tttctcttct acagaagaag aaagttgtcc tatgcggtcg
     5701 taatcggctt tgcttacagt atccggattt ttctcgacca gggcaagcag atctttttgt
     5761 tcttgctgca gagtttcgag ttcgcggccc agggttgcgt gttttttctc cagcgccttg
     5821 agttctttgt ctcgagcttt cttgtctgct ttcgccttac caccgggcgg tggcgcagct
     5881 tgcaccgttt ctacttgttc taccggttct gagtcatcgg atgcttctgc gcgatcgagt
     5941 tcttcgttga ggtagtagac gtagtcttca tacgagcctt cgtagagacg aattgccttg
     6001 ttcttcactt ctagaatgcc tgtggccacg ccgttgacga atgttctgtc gtggctgacg
     6061 aagaatactg tgcctttgaa atcctggaga gcttctccga gcacttccac agtatcaaaa
     6121 tccaggtggt tggttggttc atccagaaga aggatatcgc atttctgaag cagaagagaa
     6181 agcaagagca cgcgcgatct ttcgccaccg gataagatgg atactttctt ttttgtatcg
     6241 tctccgccga aaaggaaagc tcccgccatg ttcagtattt cttgtctggt catggatttg
     6301 ggcgcataac gttcgagata ctgaagaaca gtctcttcgc cgcctaacgt ttcatacacg
     6361 tgctgcgcat agtagccgat cttcgtgtcg taaccccagc gcacttctcc tgccagagca
     6421 ggcagggatc cttgaattgt tttgaggaat gtagacttac cctggccgtt gtccccgagg
     6481 atcgcaaagc gatctccgcg tttcaattca aagcttatgt tgtccgcgat cattctgtct
     6541 ccgtatccaa tccggagatc cttgcacttg agtgcaacgc cgccctgtct atcaggtgat
     6601 ggaattcgca tgcgaacact tctggccgct cccgccactt cctttgtttt caggcgggaa
     6661 atttgcttca ttttggactg agcctgggtt gctttggatg ctttcgcacg gaatcggtct
     6721 acgaagttct ggagttcttt tctgcgtgct tcaatgttct ggttttctct gattgcctgt
     6781 tcgcgcactt ctcgtttgta ttccagatac tcttccacat ttccagggaa gcttgcgatt
     6841 gttttgttgt ccacttccag agtgtgcttg catgtcttca tcaggaattc gcggtcatgc
     6901 gagatgatga gagcggcccc gcgaaacgtt cgcagaaaat gttcgagcat cagtagagtt
     6961 ttcaagtcca ggtagttggt tggttcatcg agcatcaaca ggttgggttc ttccagcatc
     7021 atcgctgtca gttttacgcg catgcgaaag ccgcctgcca gacccgagat ctttgctccc
     7081 agcatgtcgc ctttgatctg aaatcttccg gcgtactttc cacatctcca ttcttcctgg
     7141 tcagtggtgc gcatcagaaa gtcgatgacc gtctcatcag gtagatgcgt gtctttctgt
     7201 tccagataac tgagccgaag atcgctcgaa agccagacac ttccggagtc cgcttcctct
     7261 ttggaggtga tgatattgaa gagagtcgat ttgccggcgc cattgcgtcc gattacaccg
     7321 aatttttgat cttctgagat gactacttct gcgtcgtcga agagaactct gggtccatat
     7381 tgtttgtgga gcccgctgat ttgtatgaga gcggccatcc gttacgtttt tttttggccc
     7441 ccaggcataa attacttttt agttgttcga aatcgcccct tctcttttgt gttttccatg
     7501 cgcgtactga ttgcactttt attcctggtt ccggccggtc ttttcgccca tcccgggcat
     7561 gcagccggtc cactttctgg tctgtggcat ccattcaccg gcctggatca tttgcttgtg
     7621 atggttgcgg ttggttttgt agcctctcgc ctgggcggaa aagttcgcct cttcgccccc
     7681 gctgcgtttc tactggcaat gatttcaggt gcctctgcat cctgggcagg cgtctcgctt
     7741 ccgggagttg agcctctggt acagctgagt ctcgcagtct tcggtgttct tttgatggcg
     7801 ggctttcgca tgcactccgg tttcttcctt gtgctcgtgg gtgttgccgg attctttcat
     7861 ggcgccgcgc atgcatcgga aggtccggct gcgatcagtt atgcgatcgg atttctagcg
     7921 tccacagctg ccttgcatgt ggcagggctg ctgcttggaa acattcagac cagagcgggc
     7981 agtgttcttt cgcgcgcagt cggtgccgcc atgatcgcat tggccatggc gggctaagcc
     8041 ccgactttat tcggccttca aatcgcgggt catcaaatcc atgatggcct gcttcacagg
     8101 tttgtcttca aagagtacct tgtatacctg agtggtcaca ggcatttcta tcttttctct
     8161 ctgtgcgatt tcatgaacgt gtcgcgccgc ctctacacct tctgccacag cagtcatccc
     8221 tgcgaggata tccttgagag tctttccttg cgccagactt tcgcctacga aatgattgcg
     8281 actcatttta gaagagcagg ttgtgatgag atcgcccatg cctgcgagtc cgtaaaatga
     8341 atctgccttc gcgccaaagt gagttccaaa tcggatgatc tccacaagcc cgcgaaccat
     8401 cagggcagac ttcgtattgt ctccgaggtc cagaccatcc acaatgccgg ctgcaatggc
     8461 gatcacgttt ttgagagttc cgcccagttc agatgcagcc atgtctgtgt tcgtgtacac
     8521 gcgaaagtaa ggagtactga agaatttttg agcagctttt gcgatttctt cattgggaga
     8581 tgcgatgact gtggtggccg gtttcttgag cgcgatttcg cgtgcgatgt ttggcccgga
     8641 caatactgcg acgcgtgttt tcatttcagg cggaagattc tctatgagca cttcggatgg
     8701 tctgcggttg tctgccggat tcaatccctt tgtggcagaa acgatgacta catcgcgggg
     8761 aagaaacgga tgagccttct gcacagtgga ggcgtaaaat tgagacgcaa ccacaaatac
     8821 cacaatcttg ctatccacgc atgcctcttt taagtccgtg gtggcgcgta ttttatcgga
     8881 tagcttcacg cctgcaaaca gagctttgtt ttcatgttcc tcgttgatcg tgcgcgcgat
     8941 ctctggatcg tgagaccaca tcaccacatc gtgtccgttt tcagccagaa ttcccgccag
     9001 agttgttccc caggcaccca caccaaacac agttgtcttc atggcggatt cttggctcca
     9061 cacgatccgc ggtcaagagg aag
//
LOCUS       DAORFF010000079         9059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3632_length_9059_cov_3.478232, whole genome shotgun sequence.
ACCESSION   DAORFF010000079 DAORFF010000000
VERSION     DAORFF010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9059
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3632_length_9059_cov_3.478232"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PKK76_04875"
     CDS             <1..770
                     /locus_tag="PKK76_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67832.1"
                     /translation="VIAGPTVSFSPEARTAVLDYLKRGGKVLASINPAGPEDMSWLLE
                     AADSHLRFKNAFLSSEAVWPGIPITDSLETHRITENLSVAGRAFIAFPECGYFAEGSV
                     PSAQPQILQENNQKMKQTIFLYTKFNTVVDTNRDGRKEPGEESGRFPLGVAFEYPDHK
                     GARLVVYSGIGWMTELGLRFPIDQRNIILAQDSLSFLMENPIAAGLVPEQRKTRSIPV
                     TEGLKFRNFILGVVAFPLSTVLLTLGGVWLYRRRRKK"
     gene            767..1852
                     /locus_tag="PKK76_04880"
     CDS             767..1852
                     /locus_tag="PKK76_04880"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNL67833.1"
                     /translation="MNRRLGIILGVGSLLILGLLLLFGPGTQKDKPDAIFWKKDFDAI
                     EYVSAGKNRATSLRLVRKAGLIRDEFTVEGSGKSARRGNYNAKNIFTDFSKPRNAGQY
                     KLSPERVEELGFTAESPQVILYEKIGSAPLRLTVGKKNQTGNTFVRCDDPSFKDQVLL
                     MPSYLFERFDKPIKEFREQRFLVYSTDSFTEELEVTIDGGGPIKSYWMKQEKYKKDGI
                     ELPRWRDRQGTEIPLNQASALENAVREIQILEYRDADGLPSAEDAWKRAGKDTTVVKV
                     KVKGQDETIIRLRSSYVIQNGKDSLITVQSTVEPGTDFVRANVEHDLASKIDQIVESL
                     QKKAEVEKRNHEQKDQPNASPAVEPTR"
     gene            1849..2385
                     /gene="lptE"
                     /locus_tag="PKK76_04885"
     CDS             1849..2385
                     /gene="lptE"
                     /locus_tag="PKK76_04885"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNL67834.1"
                     /translation="MTIAQRVCILCVIAAQGTCRLLQHTDLSDRDPATRTIFVHNFSN
                     ETFQPDVQAEITDWLKKELSRRDNFKVVSNREDARIWVYGEVVVFSKEGRMYDNFRNP
                     TRYEMLTTVHIKMRENPARSTGGPLLDKSGEVTQTIQFSEKEGYKEPEFQARQRMLRI
                     LASKISDALESEYLEWHP"
     gene            2369..3424
                     /locus_tag="PKK76_04890"
     CDS             2369..3424
                     /locus_tag="PKK76_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNL67835.1"
                     /translation="MSGILRIAYGALAVVCSAGLLETTAGAFASRVDPVTALDPVTRY
                     AYKPGLDLVSTAEEYRITFRTDSMGLRVCGSGEVGREPPAKGGFYFIGDSFTEGLGVD
                     CEKTFAAAVARSKRKDFFNAGMHGGAPPFYVLRNQKFLKRLTPEAVFVQIYDNDATDM
                     DAMSEFISLGQDVRVKPLSEKESLRIHLTEHSSFLSALRIRLKENHSPWFFRAGTAPA
                     ELPPGTDASKNSDSFGFYGKTKMESPWSNRLEMLAASMDRMIQDLRVLAPDARLVLIH
                     IPDRTIYQYSLVSQEQNAVRAMVRLAAARSGAIFVDGQVALSRDPGTKYFPGNGHINA
                     DGHAAIARAILVQLGTR"
     gene            3507..3971
                     /locus_tag="PKK76_04895"
     CDS             3507..3971
                     /locus_tag="PKK76_04895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNL67836.1"
                     /translation="MYPKIRITLPAGTIECELYNDVAPRHAERILTLAGKGFYDGILF
                     HRIVPDFVAQAGDPKTKNGLNQPGVGAGGSDLPDLPLEVSPLQKNTRGSLAMARTQNP
                     NSANSQFYIVLKDAPHLNMQYTVFGRVLENGMDVVDKIKMGDAIIKMEEIKG"
     gene            complement(3974..4999)
                     /locus_tag="PKK76_04900"
     CDS             complement(3974..4999)
                     /locus_tag="PKK76_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNL67837.1"
                     /translation="MKRLVFQKKGIIDWEECPAPVITGPNQAIVRPLAIARCDLDIPI
                     VLGQTLFRPPFAIGHEFVGSIESVSEDLAGQFQKGDRVAVAFQISCGTCPMCTGGLSK
                     NCTTVTPAANYGMAPAAKEFGGALADFVKVPFASSMLLRLPPNVPAASVASLSDNIAE
                     AWKLAGSVLSNRPSSTVLILGGVASSIGLYTAMLAKIMTKGSVLYMDTDPARVLHAER
                     LGIQAEHVKEFPKSRGKFDLVCECAASEAGWTCATRSVAPAGVLTTASIFWTNKLEIP
                     FLEFYNGGVELRISRVDSREMMEKMLTLISDGTYDPGLVTTSTASLADAKEAWMEPAT
                     KLVITNE"
     gene            complement(5106..5954)
                     /locus_tag="PKK76_04905"
     CDS             complement(5106..5954)
                     /locus_tag="PKK76_04905"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNL67838.1"
                     /translation="MKIFVYGATGMVGQNVVNQLLAAGHEVFAGSRKPESGKASRGLT
                     WVKADAANPQAGVEILDKVDRAFFIAPPGFTDQYAILSPWLNRAKEKKLQKVVLMTAM
                     GVEHAPAEAPFRKVELMLEGSGLNYNIVRPNWFMQNFQTFWIGGIKSDGKIYFPGGDA
                     KASFIDARDISSSVVSLLTEDKFNNQAFALTGPAAVTHDEVAAALSSVSGKSITYVNV
                     TPEDFKKGLLSGGVPNDYADFLVFIAGNLREGHASPVTATVKQITGKEPIAFQQYAND
                     NQAAWK"
     gene            6082..6999
                     /locus_tag="PKK76_04910"
     CDS             6082..6999
                     /locus_tag="PKK76_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNL67839.1"
                     /translation="MDLLSGILQQARWTNHMLLRKTIRGSWGFRFPCDRSGGFHVITL
                     GSCYLVHDEEEVKLEKGDLVFVMRGVPHDLVSEPGQKVLDISKFSEASMRTRNGPVVQ
                     ITSMRYEFPDGDAHSFFRELPPIVLVRGSEIPPHHPLQTALSLLSAEQGNENGSALIL
                     ERLTDIMLFYALRHWLGSHPARQPGFRSAMLDEKISSVLDSVHRKPAHAWTLDGLARM
                     VGMSRASLASRFRGAMGMTVMDYVARLRLDAGRVLLEDESMTLEDAAQAVGYSSAFAF
                     SKAFKRVFGRSPRSPLPANSSRYKLTAVR"
     gene            complement(6962..8989)
                     /locus_tag="PKK76_04915"
     CDS             complement(6962..8989)
                     /locus_tag="PKK76_04915"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL67840.1"
                     /translation="MIGRLLIVLLLFTSYGSVFAEMLPPEKWSYIQTGRPSLWTWKEL
                     ESKTWTRATKLSFSGRQGNMVYLKTTIPSLEIADPSIYISLVSQSFEVYCNGNMIYQF
                     GNPNRGREGFSGWPAHIIPIPKSCDAKSTIIFKIYSDLMLLGFSGPSDPEYANRADIE
                     AKIVKRDIDRFSLGFLYIMTGAFSCFMYARRRKQISYLTFGAFALCAGIYSVTNRTLE
                     IQHLIWHAPMALYYIEYTTLFLLPATIALFFREIAVEGRVYAAIALVHVLYFSGAMIL
                     DLTLVPLYHALLPFFNVALISAIIGLIFMVREIKRGNKEARTLSFGLTLFVLTAIYDL
                     LGNLGYIPWPRQLVSWGFFAFMLSMIYVLEVRFSADQDRLTLYAAELEGAKANLEEKV
                     ELRTDELRKSLETVRELKTAQDGDYFLTSLLLRPLARNAADSGIIEVDTLTKQKKQFE
                     FKNRKYEIGGDISISDTIELSGKRYIVFANADAMGKSLQGAGGAIVLGSVFNSLIIRS
                     RFQGDYAKRTPVEWITQAFLELQHVFESFDCSMLVSCVLGLVEEETGLLTYVSAEHPS
                     VVLFRDAKASFLDTETAHLKLGTPHTITDVHIRSFQMKKGDHIIAGSDGRDDLHLGGA
                     NIRIMNENELLFLEIVERAKGDLKEIAAQLEKEGSLTDDLTLLRLAYNAKN"
BASE COUNT         2225 a         2175 c         2363 g         2296 t
ORIGIN      
        1 tcgtgattgc agggcccact gtttcttttt cgccggaagc gcgcacagcg gttctggact
       61 atctgaaaag aggcggcaag gttcttgctt ccatcaatcc cgcaggaccg gaagacatga
      121 gctggttgct tgaggcggca gattcgcatc ttcgcttcaa gaatgctttt ctttcgagcg
      181 aagctgtatg gccggggatt ccgattactg attcgcttga gactcatcgc ataacggaga
      241 acttgtctgt ggcgggtaga gcgttcattg catttcctga atgcggatat ttcgcggaag
      301 gttctgtccc ttctgcgcag ccacagattc tacaggaaaa caatcagaag atgaagcaga
      361 ccatcttcct ctacacaaaa ttcaatacgg tagtggatac gaaccgcgat ggaagaaagg
      421 aacccggaga agaatccggt cgattccctc tgggtgttgc atttgagtat ccggatcaca
      481 aaggagcgcg actggttgtc tactcgggca ttggttggat gacagaactg ggtctccggt
      541 tccctatcga tcagagaaac atcattctgg cgcaggacag tctctccttc ctcatggaaa
      601 atccaattgc tgcggggctc gtcccggagc agaggaaaac caggagtatt cctgtaacgg
      661 aaggtctcaa atttagaaat ttcattctgg gagttgtagc tttcccgctt tctactgtcc
      721 ttctgacgtt aggcggcgtt tggctttacc ggaggcgtcg taagaaatga acagacgact
      781 cggaatcatt ctgggcgtag gatctctgct gatcctcggg ctgttattgt tgtttggccc
      841 cggcacgcag aaagacaaac cggacgcgat cttctggaaa aaagattttg atgcaattga
      901 atacgtatct gcgggcaaaa atcgtgcaac ttctttgcgc ctggttcgaa aagcaggcct
      961 gatcagggac gagtttacag ttgagggttc gggcaaatct gccaggcggg gaaactataa
     1021 tgcaaaaaat atcttcaccg attttagcaa accgcgcaac gcaggccagt acaaactatc
     1081 tcccgaaaga gtagaagagc ttggattcac tgcagaatcg ccgcaagtca tcctgtacga
     1141 aaaaataggt tctgctccgc ttcgtttgac ggttggaaaa aagaatcaaa ccggaaacac
     1201 gtttgttcgc tgcgatgatc cctcttttaa agaccaggtg ctgttgatgc cttcttattt
     1261 gtttgagcgc tttgacaaac caatcaaaga gtttcgcgag cagcgttttc tggtctacag
     1321 cacggattca tttacagaag aacttgaggt caccattgac ggtggaggcc cgatcaagtc
     1381 ttattggatg aagcaggaaa aatacaagaa agacggcatc gaactgcccc gctggcgcga
     1441 tcgacaagga acagaaatcc cgcttaacca ggcttctgct ctcgagaatg cagtgcgcga
     1501 aattcaaatt ctggagtatc gcgacgcgga cggacttccg tctgccgaag atgcctggaa
     1561 gcgtgctgga aaagacacaa ccgtggttaa agttaaggtc aaagggcagg atgaaacaat
     1621 cattcgcctt cgttccagtt atgtgattca gaatggcaaa gactctctta taacagtgca
     1681 atctactgtg gagccaggca cagatttcgt gcgcgcaaac gtggagcatg atcttgcaag
     1741 caagatagat cagatcgtcg agtcactgca gaaaaaagct gaggttgaga aaagaaatca
     1801 cgagcaaaaa gatcagccaa acgcaagccc ggctgtagag cccaccagat gacgattgcg
     1861 cagcgagtct gcattctatg tgtgatcgct gcacagggaa cctgtcggct tttgcagcac
     1921 actgatttgt ctgacagaga tccggcgacg cgaaccatat tcgtacacaa tttctcaaac
     1981 gaaacatttc agccggacgt acaggcagag atcacagact ggctcaagaa agaactcagt
     2041 agaagggata atttcaaagt tgtgagcaat cgagaagatg ctcgtatctg ggtttatggg
     2101 gaggtcgttg ttttttctaa agagggcagg atgtatgaca actttagaaa cccaacacga
     2161 tacgaaatgt tgaccaccgt gcatatcaag atgcgcgaaa atcctgctcg ttccaccggc
     2221 ggacctttgc tcgacaaatc tggtgaagtc acgcagacca ttcagttttc agaaaaagag
     2281 ggatacaaag aacctgagtt tcaagcaagg cagagaatgc tgagaatcct tgcttccaag
     2341 atctcggatg ctctggagtc agaatatctt gagtggcatc cttagaatcg cctacggcgc
     2401 gctggcggtt gtctgtagcg cgggtcttct tgaaactaca gcgggggcgt ttgcctctcg
     2461 cgttgatcca gttactgccc ttgatccggt aacaagatac gcgtacaaac cgggtctcga
     2521 tctggtttca accgcagagg aataccgcat aacgttcagg acggactcga tgggtctaag
     2581 ggtgtgtggt tccggcgaag tgggtcgaga gcctcccgca aagggcggct tctattttat
     2641 aggtgattcg tttacggaag gactgggcgt agactgcgag aaaacattcg cagccgcagt
     2701 agcgcggagc aaacgcaaag acttctttaa cgccggcatg catggagggg cacctccttt
     2761 ctatgtcttg agaaaccaga agtttttgaa acgcctcaca ccggaagcag tctttgttca
     2821 gatttatgat aacgatgcga cagatatgga tgcgatgtca gaattcatct ccctcggtca
     2881 agatgtgcga gtcaaacctc tatcggaaaa ggaatcgctt cgcattcatc ttacagaaca
     2941 ctcttcgttt ctttctgccc tgcgaattcg gctgaaggaa aaccatagtc cgtggttctt
     3001 tcgcgcgggg acagctccgg ccgagctgcc tccaggaacg gacgcgagta agaattcgga
     3061 ttcgtttggt ttttacggaa agacaaagat ggaaagcccg tggtcgaatc gcctggaaat
     3121 gctggctgca tctatggatc ggatgatcca ggatttgcgc gttcttgcac cagatgcccg
     3181 gcttgtgctc atccatattc cagacagaac catctaccag tattcgcttg taagccagga
     3241 gcaaaatgca gtgcgggcca tggtaaggct cgcggcagcc agatcaggcg ctattttcgt
     3301 ggatggtcag gtcgcgctat cgcgcgaccc aggtacaaag tatttccctg gcaatggcca
     3361 tatcaatgcg gatggtcatg cggcgatcgc acgtgcgatt ctagtgcaac tgggcacgcg
     3421 ttaggcgatc ttgtcggacc ttttcgcgta gtctgagaaa ttgtttttct ggcggacggg
     3481 acaaagggtt cagaaatctt cagtccatgt atccaaaaat tcgaattact ctacctgcag
     3541 gaaccattga gtgcgaactc tacaacgatg tagcgcccag gcacgcggaa cgcattctca
     3601 ctctggcagg caagggcttc tacgatggga ttttatttca tcgcattgtt cccgatttcg
     3661 tagcccaggc cggagatcca aaaacaaaga atgggttgaa tcaaccgggt gtgggtgcag
     3721 gtggttcgga tcttcctgat ctgcctttag aggtaagccc ccttcagaag aacacgcgtg
     3781 gcagtctggc catggctcga acacagaatc caaattccgc taatagtcaa ttttatatag
     3841 tattgaaaga cgcgccgcac ctgaatatgc agtacaccgt attcgggcgc gtcctcgaaa
     3901 acggtatgga cgtcgtggac aaaatcaaga tgggagacgc catcatcaag atggaagaga
     3961 tcaaaggata gatctattca tttgtgatca ccagcttggt cgcaggctcc atccaggcct
     4021 ctttcgcatc cgcgagagag gctgtgctgg ttgtaaccag gccggggtca taggttccat
     4081 cagatatcag agtcaacatt ttctccatca tctctcttga atccacgcgg ctgattcgta
     4141 gctccactcc accattataa aattcgagga acgggatttc aagcttgttg gtccagaaga
     4201 tggacgccgt tgtcagcaca cccgccggag ctacggatct tgtggcgcaa gtccagcctg
     4261 cctcacttgc agcgcattcg cagacgaggt caaactttcc gcgcgatttt gggaactctt
     4321 tgacgtgctc tgcttgaatt ccaagtcgct ctgcatgaag gactcgcgcc ggatcggtgt
     4381 ccatatagag aacagatcct tttgtcatga ttttcgcaag catagcagta tagaggccaa
     4441 tgctcgaagc cactccgcct agaatcagaa ctgtagaaga tggacgattg ctcagcactg
     4501 agcctgccag cttccacgct tctgcaatat tgtcgctcag gcttgcaaca gacgccgcag
     4561 gaacgttagg cggcaatctc agaagcatcg aactggcgaa aggtaccttt acaaaatcag
     4621 cgagggctcc tccaaattct tttgccgcag gtgccattcc gtagttggcc gccggtgtaa
     4681 cagtagtgca gttcttggat aggcctccgg tgcacatggg gcacgtgccg catgagatct
     4741 ggaatgcaac ggccactcga tctccctttt gaaactggcc agccaggtcc tcagaaacgg
     4801 attcaataga tcctacgaat tcgtgtccga tggcgaaagg agggcgaaac aatgtctgcc
     4861 cgagtacaat gggaatgtca aggtcacagc gagcaatcgc cagcggccga acaatggcct
     4921 ggttgggccc tgtgatgaca ggtgccggac attcttccca atcaatgatt ccttttttct
     4981 ggaaaacgag tcgcttcatg ttccgcctcc gggattagac cgatctgtct aatccggagt
     5041 ggaaggtgga ttctgtctag aaaaaaattc aagccgtggc gggtgccacg gcatcgtttc
     5101 tcaggttatt tccaggcagc ctggttgtcg tttgcatact gttggaacgc aatcggctct
     5161 tttcccgtga tttgcttcac ggttgctgtg accggggatg catgtccctc cctgaggttc
     5221 cctgcaatga aaaccaggaa gtccgcgtaa tcgtttggaa ctccgccaga gagaagacct
     5281 ttcttgaagt cctcaggcgt tacgtttacg tatgttatgc tttttccgct gacgctggag
     5341 agagcagctg ccacttcatc gtgagtgact gcggcaggtc cagtgagcgc gaacgcctgg
     5401 ttgttgaatt tatcttcagt gagcagagaa acaacggatg aagagatgtc tcttgcatct
     5461 atgaagcttg ctttcgcatc tccgcccgga aagtagattt taccgtctga tttgatcccg
     5521 ccgatccaaa atgtctggaa gttttgcatg aaccagttcg gtctcacaat gttgtagttc
     5581 aaaccggagc cttccagcat gagttctacc ttgcgaaagg gcgcttctgc tggagcgtgc
     5641 tccactccca tcgcagtcat cagtactact ttttgaagct tcttttcttt ggcgcgattc
     5701 agccaggggc tgaggattgc atactggtct gtaaagccgg gcggggcgat gaagaatgct
     5761 ctgtctactt tgtccaggat ttcaacgcct gcttgcggat tggcagcgtc cgccttcacc
     5821 caggtgagcc ctctggacgc ctttcctgat tctggcttgc ggcttccagc aaaaacctca
     5881 tggcctgcgg ccaacagttg attcactacg ttttgcccaa ccattccggt tgcgccatat
     5941 acaaatattt tcacactttc ctccgttctg atctaagtgt agcgcacaga ttgcgttctg
     6001 gcaatgtccg gacgtccaaa gaagcatatc caggagtata aatcattgga cgtttgaaca
     6061 tcccggtata tttttgggat catggatctt ctttccggta ttctccagca ggcacgctgg
     6121 accaatcaca tgcttttgcg aaagacaatt cgaggctcct ggggctttcg ttttccctgc
     6181 gatcgcagcg gtggctttca tgtcatcact ctgggcagtt gctatctggt gcacgatgaa
     6241 gaggaagtga agcttgagaa gggggatcta gtatttgtca tgcgcggtgt tcctcatgat
     6301 ctagtttcag aaccaggcca gaaggttctg gacatatcca aattttctga ggcttctatg
     6361 cgcaccagga acggccccgt ggtgcagatc acttcaatga gatacgaatt tccggacgga
     6421 gatgcgcatt cgttcttcag agaattgccg cctatcgttc ttgtgcgagg atcagaaatt
     6481 cctcctcacc atcctcttca gactgcactt tctcttcttt ctgcagaaca gggcaatgaa
     6541 aacgggtccg cccttatcct cgagcgacta actgatatta tgctattcta tgccctgcga
     6601 cactggcttg gatcacaccc tgcgcgtcag cctggatttc gcagcgccat gctggatgaa
     6661 aagatttcat cagtattgga ttctgttcac agaaagcccg ctcatgcctg gactctggat
     6721 ggtctggcgc gtatggtggg aatgtccagg gcttcgcttg cttctcgctt tcgaggcgcc
     6781 atgggaatga ctgtgatgga ttatgttgcg agactgaggc tggatgccgg acgcgttttg
     6841 cttgaggatg aatctatgac tcttgaggat gcagcccagg cagttgggta ttcctctgcc
     6901 tttgcttttt ccaaggcttt caagagagtc tttggcagat ctccaaggtc tcctttgccg
     6961 gctaattctt cgcgttataa gctaaccgca gtaaggtgag atcgtctgtg aggctgcctt
     7021 ctttttccag ctgagcggcg atctctttga gatctccttt tgcgcgttcc acgatttcaa
     7081 gaaagaggag ttcgttttcg ttcataatgc gaatattcgc tcctcccaga tgtagatcat
     7141 ctcttccatc tgagccagca atgatatgat ctcctttttt catctggaag gatctaatgt
     7201 gaacgtctgt gattgtatga ggggtgccca gtttgaggtg tgcagtttct gtatccagaa
     7261 aggaagcttt tgcgtcacgg aacagtacta cagacggatg ctctgcggaa acgtaggtca
     7321 acagtccagt ttcttcctct accaggccga gcacgcagga aacaagcata gaacaatcga
     7381 agctctcgaa tacgtgctgc aattccagaa aagcctgggt gatccattct acgggcgttc
     7441 tttttgcata gtcgccctga aagcgcgagc gaatgatcaa tgaattaaac accgagccaa
     7501 gtacaatggc cccgccggcg ccctggaggg attttcccat ggcgtccgca ttagcgaaga
     7561 caatgtagcg tttgccggaa agttcaattg tgtcactgat tgaaatgtcc ccgccgattt
     7621 catatttccg attcttgaat tcaaattgct ttttctgttt tgtcagcgtg tctacttcta
     7681 tgataccgct gtctgccgcg ttgcgcgcga gaggacgcag aaggagagaa gtgaggaagt
     7741 agtccccgtc ctgagcagtc ttcagttctc gcactgtttc cagagatttt cgtagttcgt
     7801 ctgttcggag ctctactttt tcttccaggt ttgcttttgc cccctccagt tcagcggcgt
     7861 acaacgtcag gcgatcttga tcggcggaga atctcacttc cagaacataa atcatggata
     7921 gcatgaacgc gaaaaatccc caggaaacaa gctgtctggg ccacggaatg tatccaagat
     7981 ttcccagaag gtcatagatc gcggttaata caaagagagt taggccaaag ctgagcgttc
     8041 gtgcttcttt gtttccgcgt ttgatttcgc gcaccataaa tatcaacccg atgattgctg
     8101 aaatcaaggc tacgttgaaa aagggaagaa gtgcgtggta aagaggcaca agagtcaggt
     8161 caagaatcat agcccctgag aaatagagca catgcaccag agcgatcgct gcgtagactc
     8221 gaccttccac ggcgatctcg cgaaagaaga gtgcaattgt tgcgggaagc agaaagagag
     8281 tggtgtactc tatatagtag agagccatgg gtgcatgcca gatcaaatgc tggatttcaa
     8341 gagtgcgatt ggttaccgaa tagattcccg cacaaagagc gaatgcgcca aaagtgaggt
     8401 aagagatttg ctttcttctc cgcgcgtaca tgaaacagga aaaagctccg gtcatgatat
     8461 aaagaaagcc aagagagaag cgatcgatgt ctcgtttgac tattttcgct tcgatgtccg
     8521 cgcgattcgc gtattctgga tctgacgggc ccgaaaatcc gagtagcatc aggtcagagt
     8581 agattttgaa aatgatagtg ctcttcgcat cgcaggattt tgggatggga atgatgtgag
     8641 ccggccatcc gctgaaccct tctctgccgc gattgggatt tccaaattga tagatcatgt
     8701 ttccattgca gtacacttca aatgattggg aaactagaga gatgtaaata gacgggtccg
     8761 cgatttcgag cgaaggaatg gttgtcttta gatataccat attcccctgg cggcctgaga
     8821 aggagagttt tgtagccctg gtccacgttt tggattcaag ttctttccat gtccagagag
     8881 aaggccttcc cgtttgtatg taggaccatt tttccggagg cagcatctcc gcgaaaacgg
     8941 agccgtaaga agtgaacaag agtagaacga tgagcaggcg cccgatcacg gctcatcgta
     9001 aacctgggcg attgcgccgc aacctttgtt ttcgcattgc agttcgtctt cgcgtcatg
//
LOCUS       DAORFF010000080         8957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3690_length_8957_cov_3.923388, whole genome shotgun sequence.
ACCESSION   DAORFF010000080 DAORFF010000000
VERSION     DAORFF010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8957
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3690_length_8957_cov_3.923388"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKK76_04920"
     CDS             <1..346
                     /locus_tag="PKK76_04920"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNL67841.1"
                     /translation="LTPANVAGFLILDRHFPRSIRFCITEGERSLRELTGGDPQAFRN
                     EAEKRMGSFRSQLEYTEVGDIFRHGLHEYLDLIELRLNEIGEAIQEVFLVPPDTSSMP
                     AGAVFPQAQQQA"
     gene            complement(349..1707)
                     /locus_tag="PKK76_04925"
     CDS             complement(349..1707)
                     /locus_tag="PKK76_04925"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL67842.1"
                     /translation="MTFVGNLRRVVGFRTARDYLLGTALFAVIYVTLDLFSDTKPPHW
                     SLLIAYVAYCTVYTLFASWRLVSGAWTPRRWARTTLVFCIPISLFVGYTASTIPHLFL
                     LFGLFIIGIGLPMIGQLRAALWVLTLAVYLGTYLFGLWKLHPEYLFNNKQSVALSTVM
                     FAAITYWLARISWFVEEASKKSTRTLVVTRKQKRELDRISKELRLKDKSLARDLNFAR
                     NFQLNQLPDLEIFNTGGFKSAMHYIALDTVGGDIIDMVRFSPDRIGVFVGDVSGHGVR
                     AAMVAMMARVGFANLCKMSDDPAIVAAALNAFLCRSLENDRSCYMTAIYVIIDRSTMQ
                     MHLTCAGHTPAILIRTNHTIEEIDTHPSLFLGVEPDQGYKTTTVSVQPSDKLLLYTDG
                     LTEPLNDAGEQYGERVFGEFLRSRAGLEPSEILAGLAVSMEGFTTENLRDDVAIVCVK
                     VE"
     gene            complement(1787..2593)
                     /locus_tag="PKK76_04930"
     CDS             complement(1787..2593)
                     /locus_tag="PKK76_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67843.1"
                     /translation="MNSTQFTSRYSFGLFVLLGAAFFATPASAQETYFAVPDGIAMNR
                     ILPAIRFGAEYKSSDLDQHKYYARTQTVDAYGEFAFNENVSIAGYLPWTRKKETNIKS
                     HTRFDNLGLGVHGALPGSTFIPVAGLDLTLPTGSEKVGIGSKRIFNIEPYAGLAFHDG
                     IFSITGLAKYNTQSNRRFREDPEKQDDFERTWLANVGLGLNFEVTDLLLEYQYRYTYD
                     PDPKRKSIHTIAPGLNLKVDHFIFSFSVPCALSKQREFDYGVILRAGMRF"
     gene            complement(2590..3441)
                     /locus_tag="PKK76_04935"
     CDS             complement(2590..3441)
                     /locus_tag="PKK76_04935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNL67844.1"
                     /translation="MVDSQSILLRFSILFQAALILAATVHCEHPKQSALDSIALTSYC
                     DLLAVCQKSDAKFGMIGDSWTDFAGGLPVQIDLHDWLVKRGYTIQASVIAGHTAEAEL
                     SYVRGFDRVIEQSPHIKYMLVCLGGNDLLANNRNYYVNGTDAEMDARIARLDFVHRSI
                     IAQGDAHKIALWGGEGLTWIFSGYDYTNPDMDQSCVRGSLNAGMPLSQAPAQQQRIID
                     RYQTYLNSLPARAPNARSIDLRGTLGGPTQSLVGLKFDCIHPNSAGFYLLAQRYAENL
                     EQMTPEK"
     gene            complement(3435..4301)
                     /locus_tag="PKK76_04940"
     CDS             complement(3435..4301)
                     /locus_tag="PKK76_04940"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HNL67845.1"
                     /translation="MRTIFLSAVLLSWFGLSAQELVQIPKLSARVTDLTGTLSGTETS
                     ALEQKLEALETSKGSQIVVVIVPTTKPEAIEQFGIRLAEAWKIGRKKIDDGAILIIAK
                     EDRRMRIEVGYGLEGTLNDATCKRIIDEQIVPHFKKGDFAAGINAGVDAMIAKVQGED
                     LPPPVKGASKGKSSEFGHGPMSGKNAPSFGGLVIGLIVAGSVLTAFVSRLTSGLLVGA
                     GGFLLGWLFLGLSMGLLLAIVSMIATWIFGGRMGGGGGWSSGGSSGWSSGGSSFSGGG
                     GSFGGGGSSGSW"
     gene            complement(4310..4807)
                     /locus_tag="PKK76_04945"
     CDS             complement(4310..4807)
                     /locus_tag="PKK76_04945"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNL67846.1"
                     /translation="MSIGRIIRHIVASPWTLRKMLPADALKRIEDAIGRSENTHRGEI
                     RFAVEVDLNAIQILRGVSPRDRALDVFSQLKVWDTEENTGVLIYLLLADRQIEIVADR
                     GIHKKVGDEAWINIANHIESAFRAGRYEDGVIKGLEEVTKLLSTHFPAKAENRDELSN
                     KPVVL"
     gene            complement(4804..5445)
                     /locus_tag="PKK76_04950"
     CDS             complement(4804..5445)
                     /locus_tag="PKK76_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNL67847.1"
                     /translation="MYTHVRFPKAAALLAITIALATANCGYNSIQSSDEEVKAAWSEV
                     LNQYKRRADLVPNLIKVVERFAKQEKDVFQGVADARSRVGSIQATPELINDEAAFQKF
                     IAAQGQMQGALSRLMAVAENYPQLKSDRAFTDLQAQLEGTENRIAVARGRYIKSVQDY
                     NVLVRQFPNNMTARVFGYTTKPNFTVENEQEISKPPEVDFGTDKPKEPKSENK"
     gene            5493..6401
                     /locus_tag="PKK76_04955"
     CDS             5493..6401
                     /locus_tag="PKK76_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNL67848.1"
                     /translation="MASAVERILETMTAQDLAHDFSRLMELPPEMFPLLLITAGAVIF
                     VFAFRHLALRFILPRLGSSASRIKWRRNSAYLAFSILVLLLFPLWLPSLRTVAAFLGI
                     FGAGVILVLKEVIVNVAGWFFIVFRRPFEIGNRITIGAYSGDVLDIRIMDTSLIEVSS
                     RDEGGQSTGRVVHVPNSLVFTVPFANASKEFAFNWNEIRIPISPESDWKRASLLVEKT
                     AAEVVEPISEKDSRIRKSDHEYAIRYQRITPKVYVEFKHGVILLTLRHLVEPRTNRLV
                     SDRFWRILLERFQKEKRIQLHAPMPH"
     gene            complement(6429..6947)
                     /locus_tag="PKK76_04960"
     CDS             complement(6429..6947)
                     /locus_tag="PKK76_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNL67849.1"
                     /translation="MITTKRLEIIPCEIRHLEAFARSREELAKMLALRLPPSWPAFPE
                     AYAPPETAAPDPRPWGSYFFLCPALGSLVGSGGFKGKPDSTGTVEIGYEIGKEHWNRG
                     FASEATQGMIDFAFRHEEVKFVTAHTLAEQNASCTVLQKSGLTFIREINDPTDGNIWF
                     WQISKEEHAKRA"
     gene            complement(7193..8059)
                     /locus_tag="PKK76_04965"
     CDS             complement(7193..8059)
                     /locus_tag="PKK76_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67850.1"
                     /translation="MSENPYTPSFSEVEPSMPDQFDASRAMRDGWEAFKKHLGPAMGI
                     ALVWLIAYVFAANSCFGLFALPHIFAGYSIAALALVRNRPKFELLFSAFETFGPVLVA
                     GLYFALVYLVFIAAIMIFFFVAAFLVGFWGAALQVKDVDQLFKAEGANSPFVWFLIAL
                     GLLAFFLQFYVMARLQLVFPLVLEKKMEATAAFRLSWKLTAPASMRLTGMKVLFDFAL
                     PLLGIALCCVPALFVYPLALAVQGAVLLQYMGPGEAQPVTPQIAQPVPTQMPPPAPPQ
                     DSPERPQNPYGS"
     gene            8044..8652
                     /locus_tag="PKK76_04970"
     CDS             8044..8652
                     /locus_tag="PKK76_04970"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNL67851.1"
                     /translation="MDSRSWTILAWILRTAKKISRIGKILSIIPGMVQDRLQEIQGHI
                     GDIIQRFELTTRKAPQAQQQSASRVAYSGPSFQSHINEVIERQSARHGVSPELIRAVV
                     AQESGGNPGAMSKAGAIGLMQLMPDTARSLGVNPYDMEQNVDGGIRYLKDMARKFDSL
                     DEVLAAYNAGPGAVQKFGGVPPYAETQDYVKKIRHSLRDIKS"
     gene            complement(8655..>8957)
                     /locus_tag="PKK76_04975"
     CDS             complement(8655..>8957)
                     /locus_tag="PKK76_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67852.1"
                     /translation="GEILDEHAMMANLKEKWILWLARRLPPCEDICRTLSRRLDERIG
                     IGERIKVKLHLWICEYCERFARQLELLQRLNQVEKDAQLSEDAKARIKNAIRSNGG"
BASE COUNT         2130 a         2213 c         2325 g         2289 t
ORIGIN      
        1 gttaacgccg gctaacgtgg ctggtttctt gatactggac agacactttc ccagatccat
       61 tcgtttctgc attacagagg gtgaacgttc tttgcgtgaa ttgacaggag gagatcctca
      121 ggcatttcgc aatgaagcgg aaaaaagaat ggggtctttt agatcccagc tggagtatac
      181 ggaagtaggc gatatatttc gtcatggttt gcatgagtac ctggatttga ttgagctgcg
      241 actgaacgaa atcggggagg caattcagga agtttttctg gttccgccgg atacgagctc
      301 tatgccggca ggtgctgtct ttccgcaggc ccaacagcaa gcataacgtt attcgacctt
      361 gacgcagaca atggcaacat cgtctcgcag attttcagtg gtgaatcctt ccatggacac
      421 agcaagaccg gccaggattt ctgacggttc aagacccgcc cgcgatctca agaattctcc
      481 gaaaactctt tctccgtatt gttctcctgc atcgttcagt ggttctgtaa gtccatccgt
      541 atagagaagt agtttgtcag agggttgaac gctcacagtt gtggttttgt agccctgatc
      601 cggttcaacg cccaggaaaa gggaagggtg agtgtctatt tcctcgatgg tatggttggt
      661 tctgattaag atggcaggcg tgtgaccggc gcacgtcaga tgcatttgca tcgtggatcg
      721 atcaataatt acatagatcg cagtcatgta acaggaccga tcgttttcga gagatctgca
      781 taaaaaggca ttgagtgcgg ccgcgacgat ggcggggtca tcgctcatct tacacagatt
      841 cgcaaatcca actcgcgcca tcatggccac catcgccgct cgaactccgt gaccggaaac
      901 atcgcccaca aaaactccga tgcgatcggg agagaagcga accatgtcga tgatgtcacc
      961 gcccaccgtg tcgagagcaa tgtagtgcat ggcacttttg aatccacccg tattgaagat
     1021 ttccagatct ggaagttggt tgagctgaaa gttccgtgcg aagttcaaat cgcgggcgag
     1081 gctcttatct ttgagtctca gttctttgct gattctgtcg agttctcgtt tttgctttcg
     1141 cgtcactacg agtgtgcgcg tacttttttt cgaggcctct tccacaaacc acgagatccg
     1201 cgcaagccag tacgtgatgg ccgcaaacat caccgtagag agagccactg attgcttgtt
     1261 gttgaacaag tattccgggt gaagcttcca caagccaaag agatacgttc ccagatagac
     1321 ggcaagagtc agaacccaga gagcagcgcg aagctggccg atcataggca gaccgattcc
     1381 tatgatgaaa agaccaaaca aaaggaagag gtgggggatg gttgacgcag tataacctac
     1441 gaacagagaa atgggaatgc agaagaccag cgttgtgcgc gcccatcttc taggagtcca
     1501 ggcgccggaa accagacgcc acgatgcgaa gagcgtataa acagtgcagt atgcaacata
     1561 ggcgatcaac aaggaccagt gcggtggttt cgtgtctgaa aatagatcca gggtaacata
     1621 aatcaccgca aagagcgcgg tgcccaggag atagtcccga gcagtgcgaa atccaaccac
     1681 tcgccttaga tttccgacaa acgtcacgag cagagggaaa gtcagtgctt atatatgtca
     1741 gcagaaattc tgcccaatgg tgtgttccgc tttcgcggga cggattctaa aaacgcatcc
     1801 cggcgcgcag aatcacgccg tagtcgaatt ccctctgttt ggaaagtgca cacggcacag
     1861 agaaactaaa aatgaaatga tccactttca ggttcagtcc gggcgcaatt gtatgaatgc
     1921 tctttctttt cgggtccgga tcgtaggtgt aacgatattg atattccagg agcagatccg
     1981 tcacctcgaa gttcagtcca agtccaacat tggcaagcca ggttcgttca aagtcatcct
     2041 gtttctccgg atcctctctg aaccgacggt tgctctgtgt gttgtatttc gcaaggcccg
     2101 taatggaaaa aattccatca tggaatgcaa ggcccgcgta cggctcgatg ttgaagatgc
     2161 gcttacttcc aattcctact ttttcgctcc ctgtgggtag agtcagatcg agtccggcca
     2221 caggtatgaa agtggatccg ggcaatgctc cgtgaactcc caggccgaga ttgtcgaagc
     2281 gagtgtgaga cttgatgttt gtctccttct ttctagtcca gggaagataa cccgctatag
     2341 aaacattttc attgaatgcg aattcgccat atgcgtccac tgtttgcgtt cttgcgtagt
     2401 acttgtgctg atccagatca ctggatttgt attcagcgcc aaatcgaatg gcaggtagaa
     2461 tgcggttcat tgcgattccg tctggcacag caaagtacgt ttcctgcgca gaagccggag
     2521 tagcgaaaaa agcggccccc agaagaacga aaagaccgaa tgagtatctg gatgtaaact
     2581 gtgtagaatt catttttctg gcgtcatctg ttccaggttt tctgcgtatc tctgagcaag
     2641 aagataaaac cctgctgagt tagggtgaat gcagtcaaat ttcaatccaa ccaggctttg
     2701 agtcggtcca cccagagtgc cgcgaagatc aatggaacga gcgttaggcg ctcttgcggg
     2761 aagtgagttc aggtaggttt gatagcgatc aatgatccgc tgttgttgcg ccggagcttg
     2821 cgacagtggc attccggcat tcagggatcc gcgcacgcag gactgatcca tgtctggatt
     2881 tgtataatcg tatccgctaa aaatccacgt tagcccttct ccgccccaga gtgcgatctt
     2941 atgcgcatct ccctgtgcga tgatggatct gtgtacaaag tccagacgtg cgattcgcgc
     3001 gtccatttcg gcatcggttc cgttcacgta atagtttcga ttgttagcga gtagatcgtt
     3061 tccgcccagg cagacgagca tgtatttgat gtgtggactc tgttcgatga cgcggtcaaa
     3121 gccgcggaca tagctcaatt ctgcctctgc ggtgtgcccg gcgattacag aagcctgtat
     3181 ggtgtaacct ctcttcacca gccagtcatg tagatctatt tgtactggaa gaccgcctgc
     3241 gaaatcagtc cagctgtctc cgatcattcc aaactttgcg tccgatttct ggcatacggc
     3301 gagaaggtcg cagtaggaag tcagcgcgat agaatctaaa gccgattgtt ttggatgttc
     3361 gcaatgaacg gtcgcagcga gaatcaatgc agcctgaaac aatatggaaa agcgaagtag
     3421 aatgctctgc gaatctacca tgaaccggaa gagccgcccc cgccgaagct gccgccccca
     3481 ccactgaagc ttgaaccacc tgagctccag ccgctcgaac cgcccgaaga ccagccacca
     3541 ccgccgccca ttcgtccgcc gaaaatccag gttgcaatca tggagactat ggccaggagt
     3601 aagcccatac tcaagccaag aaaaagccat ccaagcaaga atccacctgc gccaacaaga
     3661 aggccagacg ttaggcgact tacaaaagcg gtcagtacag atccagctac gatcaatcca
     3721 attacgagtc cgccaaacga aggggcgttt ttgccagaca tcgggccgtg tccgaattct
     3781 gacgattttc ctttggatgc tcctttcacc ggtggaggaa gatcctctcc ctggactttt
     3841 gctatcattg catctacgcc agcattgatt ccggcagcaa agtctccctt cttaaagtga
     3901 ggtacgattt gttcgtctat gattcgttta caggtcgcgt cgtttagggt accttcgagg
     3961 ccgtagccca cttcaatgcg cattcttctg tcttctttgg cgatgattag aatggcacca
     4021 tcgtctattt tctttctgcc aatcttccag gcttctgcga gacgaatgcc gaattgttca
     4081 atggcttccg gctttgtagt gggcacaatg accactacga tctgactgcc cttactggtt
     4141 tcaagtgctt ccagcttttg ttcgagtgca gacgtttcag tgccgcttaa cgttccagtg
     4201 agatccgtga cgcgtgcgga gagtttcggg atctgtacga gttcctgtgc agagagcccg
     4261 aaccaggaga gcaaaacggc ggatagaaaa atggtgcgca ttggattttt tagagcacga
     4321 ctggcttgtt ggacaattcg tcgcggtttt ccgcctttgc cgggaaatgc gtcgaaagca
     4381 atttcgtgac ttcttccagt cctttgatca ctccgtcttc gtatcttcca gcgcgaaatg
     4441 cactttctat atgattagcg atattgatcc aggcttcgtc tcccaccttc ttgtggatgc
     4501 cgcgatctgc tacgatttcg atttgtctgt ctgccaggag cagatagatc aacactcccg
     4561 tgttctcttc tgtgtcccag acttttagct gggaaaaaac atccagcgcg cgatctcgcg
     4621 gagatacacc gcgaagaatc tggattgcgt tgagatccac ttctacggca aagcgaattt
     4681 cgccacggtg ggtgttctct gatcttccaa tcgcatcttc aatgcgtttg agcgcgtccg
     4741 ccggaagcat ttttcgtagc gtccacgggg acgctacgat gtgccggatg attcttccga
     4801 tgctcactta ttctcgctct ttggttcttt tggtttgtct gttccgaaat caacttccgg
     4861 tggtttagag atttcttgtt cgttttccac agtgaagtta ggcttggtag tatatccaaa
     4921 gacccgtgca gtcatattgt ttggaaactg acgcacaaga acattgtaat cctgaacgga
     4981 tttgatgtag cggccgcgtg cgacagcgat tctattttca gttccttcca gctgtgcctg
     5041 gaggtccgtg aatgcgcggt cagatttcaa ttgagggtag ttttctgcca cagccatcag
     5101 gcgtgaaagt gccccttgca tctggccctg agccgcaatg aatttctgaa acgcagcttc
     5161 atcgttgatg agttctggag ttgcctggat agaccccact cgagagcgcg catccgctac
     5221 tccctggaaa acgtcctttt cctgtttcgc gaacctttcc acgactttga tcagattggg
     5281 aacgaggtct gcgcggcgtt tgtattgatt gagcacttcg ctccacgctg ctttgacttc
     5341 ttcgtcagag ctctggatgc tgttgtaacc gcaattggcg gtggccagag cgatcgtgat
     5401 ggcaagcaag gctgccgctt ttgggaatct tacgtgagta tacatgactt ggaagaagtg
     5461 acctcgaaag ggggagggtc aactaaaaaa tcttggcatc cgccgtagaa agaattcttg
     5521 agacaatgac cgcacaggac ctggcccacg atttttcgag gctcatggag ttgccaccgg
     5581 agatgtttcc gttgcttctt attacggccg gtgcagtgat tttcgtcttc gcgtttcgcc
     5641 atctggcgct tcgcttcatt ttgcccaggc ttggctcgag tgcttcgcgc atcaagtggc
     5701 ggagaaatag cgcctatctt gcattctcta ttctggttct tcttttgttt ccgctgtggc
     5761 ttccgtcttt gcgcacagtg gctgcgttcc tgggcatctt tggtgctggc gtcattctgg
     5821 ttttaaaaga agtgatcgtg aatgtggcgg gatggttttt tatagtattt cgtcgtccgt
     5881 ttgaaatagg aaacagaatc acgataggcg cttattccgg agacgttctg gacattcgca
     5941 tcatggatac ttcgttgatt gaagtttctt cgcgcgatga aggcggacag agtacaggac
     6001 gagtggttca cgttcccaac tcactcgtct tcacagtgcc cttcgcaaat gccagcaaag
     6061 aattcgcatt caactggaat gaaattcgca ttcccatttc tcctgaaagc gattggaaaa
     6121 gagcatcgct tctggttgag aaaaccgcgg cggaagtagt ggagccgatt tcagaaaagg
     6181 attctagaat tcgcaaatcg gatcatgagt atgcgattcg ctatcaacgg atcactccca
     6241 aggtgtatgt tgaattcaag cacggcgtga tcctgctgac tcttcgccat ctggtagagc
     6301 cgcgaacgaa tcgtcttgtt tctgatcgat tctggcgtat tttacttgag aggtttcaaa
     6361 aagaaaagcg cattcagttg catgcgccta tgcctcattg attcttcttc tgcgaggcct
     6421 gattcaactc aggcgcgctt cgcatgttcc tctttgctaa tttgccagaa ccagatgttt
     6481 ccgtctgttg gatcattgat ttctcgaatg aatgttagac ccgatttctg aagtactgtg
     6541 caggatgcgt tttgttctgc cagcgtgtgc gcagtgacga atttgacttc ttcgtgacga
     6601 aatgcgaaat caatcatacc ctgcgtcgct tcgctcgcga agccgcgatt ccagtgttct
     6661 tttccaattt cgtagccaat ttccactgtg ccggttgaat ccggctttcc tttgaagccg
     6721 ccggacccaa cgagtgatcc aagagcgggg caaagaaaaa agtaagatcc ccatgggcgt
     6781 ggatccggag ccgctgtttc cggtggcgcg tatgcttccg gaaatgctgg ccacgatggt
     6841 ggcaatcgca gtgcgagcat cttcgccagt tcttccctgg atcgagcaaa cgcttccagg
     6901 tgtctgattt cgcatgggat gatttcaaga cgttttgtgg tgatcatagg ttcgaatccg
     6961 gtcgacgcaa tccttgtaga atgtgcattc aaatgagcat atataagctt cgaggctccc
     7021 gttggggagt ggcttactgc aattttcaca aaccgggcga agctctaaca tacgcgcctc
     7081 ctgttgcagg aaacaagaag agaacatgga cgcaaccatt tcatcgcgcg cccgatggcg
     7141 cataacgttg agtcacctga atgtatgcgc ggcgctacgg ttgggatcaa ccttacgacc
     7201 catacggatt ctgcggcctt tcgggagaat cttgcggcgg agcgggaggt ggcatttgag
     7261 taggtacagg ctgtgcgatc tgaggcgtga ccggttgtgc ttctccaggt cccatgtatt
     7321 gaagtaagac tgctccctgc actgcaagag caagcgggta aacgaacaat gccggcacgc
     7381 agcacagggc gattccaagg agtggtagcg cgaaatcaaa gagcaccttc ataccagtca
     7441 ggcgcatgga agcaggcgcc gtaagtttcc atgagagacg aaatgctgca gtcgcttcca
     7501 ttttcttttc gagcaccaga ggaaagacga gctgcagcct ggccattaca tagaattgta
     7561 gaaagaacgc cagaagtccg agtgcgatga ggaaccatac gaaaggcgaa ttggctcctt
     7621 ccgctttgaa cagctggtct acatctttta cctgaagtgc cgctccccag aaaccaacca
     7681 gaaatgcggc tacgaagaag aagatcatga tggccgcaat gaaaaccagg tagaccagag
     7741 cgaagtacaa tcctgcgacc agtacaggtc caaatgtttc aaacgcagag aagagaagct
     7801 caaattttgg gcgatttcgc acaagtgcaa gagctgcaat cgagtatcct gcgaaaatgt
     7861 gcggcagagc aaatagacca aagcaggagt tcgcggcaaa tacgtaggcg atcaaccata
     7921 caagtgcaat ccccatcgcc gggccaagat gcttcttgaa tgcttcccag ccgtcgcgca
     7981 tagctcgcga cgcgtcgaat tgatccggca tggaaggctc tacttcggaa aaggatggag
     8041 tatatggatt ctcgctcatg gaccatcctg gcctggattc tcaggacagc aaaaaaaata
     8101 tcacggatcg ggaagatcct gtcgatcatt ccagggatgg ttcaggatag acttcaggaa
     8161 attcagggtc acataggaga cataatccag cgatttgagc tcacgaccag gaaggctccc
     8221 caggcgcagc agcagtccgc ctcaagagtt gcgtactccg ggccttcctt tcagagtcac
     8281 atcaatgaag tgattgagag acagtccgct cgccatggtg tttctccgga actcattcga
     8341 gctgttgttg cgcaggaatc cggcgggaat ccaggggcca tgtccaaagc aggtgcgatc
     8401 ggtttgatgc agctcatgcc ggacacggct cgctctctgg gcgtaaatcc ctacgacatg
     8461 gaacagaatg tagacggtgg aatccgctac ttgaaagata tggccaggaa attcgattcg
     8521 ctcgatgaag tgctcgcagc gtacaatgcc ggtcctggtg ctgttcagaa atttggagga
     8581 gttcctccgt atgcggaaac tcaggactat gtcaagaaga tccgtcactc tctacgcgac
     8641 atcaagtctt aacgttagcc gccatttgat cggatggcat tcttgattcg cgccttcgca
     8701 tcttctgata gttgggcgtc tttttctacc tgattcagtc tctgtagcaa ttctagctgt
     8761 ctggcgaatc tctcgcagta ttcgcagatc cacagatgaa gtttgacttt gattctttcc
     8821 cctattccga ttctttcatc cagtcgacgg gagagggttc tgcaaatgtc ttcgcacggg
     8881 ggcagccgac gggcgagcca gagaatccat ttttctttca ggttggccat cattgcgtgc
     8941 tcatccagta tttctcc
//
LOCUS       DAORFF010000081         8923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3715_length_8923_cov_3.927379, whole genome shotgun sequence.
ACCESSION   DAORFF010000081 DAORFF010000000
VERSION     DAORFF010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8923
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3715_length_8923_cov_3.927379"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..1365
                     /locus_tag="PKK76_04980"
     CDS             187..1365
                     /locus_tag="PKK76_04980"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNL67853.1"
                     /translation="MKDPAYILEGRRLPVGAFLGSLSRLGPVELGVLAAQAAIEAASV
                     DPGAVSSIICGNVVPSGPESLYLARHVGLKAGLPISASALNVNRLCGSGLEAIAQAAH
                     QIRAGEDLSLACGVESMSQSPYLAAGARTGIRYGDGKLIDLLSSALTDPYIGQAMGRT
                     AETLAQEYGISRQDQDAWALVSQTRAEAAQKSGKLSDEIKSVQVKDGKEMKVFDRDEF
                     VRGTAAGAKLAGLAPAFLENGTVTAGNASGINDGASAVVVCSERYLQSSGKKPLAKIL
                     GWASRGCPPEKMGIGPVHAIPAALASAGIQQEQIQLWEINEAFAAQYLAVAKSLKLDP
                     EITNVNGGAIAIGHPLGASGNRILLGLAKELRRRKLKYGCASLCIGGGQGIAMVIESV
                     "
     gene            complement(1370..2254)
                     /gene="cysM"
                     /locus_tag="PKK76_04985"
     CDS             complement(1370..2254)
                     /gene="cysM"
                     /locus_tag="PKK76_04985"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF008735.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HNL67854.1"
                     /translation="MDKAFRLSQLIGNTPLVDLSRKGSRVRLFGKLEGNNPAGSVKDR
                     PALWMMEQAELRGELKPGMRIVEATSGNTGIALAMVAAVKGYGIELIMPEDATRERVL
                     AMQAYGATVTLTDASRSMEGAIDLAHERFAEGGFWMPNQFANPDNWKSHYHTTGPEIW
                     RDTEGRVTHFVSSMGTTGTIMGTSRFLKEKNPAIQIIGVQPADGAKIPGIRRWPVEYL
                     PAIFEPARVDRVMDVTQQDAEATTRLLAKRDGVFCGMSSGGAICAASRLAEQLEDGVI
                     VTILCDRGDRYLSSDLFG"
     gene            2394..2777
                     /locus_tag="PKK76_04990"
     CDS             2394..2777
                     /locus_tag="PKK76_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67855.1"
                     /translation="MRKLFAITPFLLLAALACSKPSSSSGGAEDYKAESDKIKSQMCG
                     KMEECMQEMMKGMPENMRQMMSAKFSKESCMAQASAVPQKDKPENFTKEDMEAAKACG
                     EAMAKASCTDIKNHNVAGCEKFMNK"
     gene            complement(2832..3143)
                     /locus_tag="PKK76_04995"
     CDS             complement(2832..3143)
                     /locus_tag="PKK76_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67856.1"
                     /translation="MLTSPEKKATDKPEMFDTLGIYRRLKRARASEPVAREIAMAMGE
                     IVQSQLATKEDLLVVRRDFQDVKQELKLEISASRMETLRWMMGMMVAQLALLVSILKI
                     L"
     gene            complement(3194..4057)
                     /locus_tag="PKK76_05000"
     CDS             complement(3194..4057)
                     /locus_tag="PKK76_05000"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNL67857.1"
                     /translation="MAIREVVQARVRQLTDERSVLRVLPVHGGKGVGPFLFLDHMGPW
                     EVSPEETSDVGPHPHIGLATVTYLFEGSILHRDSVGSVQEIHPGDVNWMNAGKGIVHS
                     ERRPPSLANRAYRIHGLQAWVGLPLSQELSEPFFHHHPRRDLPHFTIDGVEISLLAGS
                     ALGVTAPAQTFSDLFYMDLRIPRGGSWTFVQDHRECALYIVEGTLRIHGQNFVSHSLV
                     VFEPGKEVKMDAQDGVIAVLLGGAPLDGPRHMWWNFVSSSRDRIEEAKRSWASGTFGE
                     IEGETGSIPLP"
     gene            complement(4060..5694)
                     /locus_tag="PKK76_05005"
     CDS             complement(4060..5694)
                     /locus_tag="PKK76_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HNL67858.1"
                     /translation="MSGMTFEEQLRSVFPDLSEIPEKDRFPGPVEQTEYLISGELKTW
                     PGPFQDIHSPVCVKAGDSHESYRIGHAPKMTREAALEALEAAVSAYDLGRGEWPTMSV
                     AHRIKHIEDFAVRMKATREQVVKFIMWEIAKSREDAEKEFDRTVDYIRDTIQALKELD
                     RNSSRFLIEQGIIGQVRRSPLGVVLCMGPYNYPLNETFATLIPALVMGNTCVFKPPKL
                     GILLYRPLLEAFRDSLPKGVINTVYGSGEEIVPPIMESGKIDVIAFIGSARVADTLKK
                     AHPHPHKLRSVLGLGAKNPAIVLKDADLDTTIPEAVTGALSYNGQRCTALKIFFVHED
                     IAETFATRLSEKIAQLTFGMPWSAGTKLTPLPVPGKSEYLDGLVKDAQSKGARVMNAN
                     GGAFYRTFFYPAVLFPVNDSMRAYHEEQFGPVIPIVPYSNLDDAMNYVYNSHYGQQLS
                     IFGSDPAVIAKMVDPLVNQVCRVNLNSQCQRGPDTFPFTGRKGSAEGTLSVSDALRSF
                     SIRSLFAAKESAQNKAIIETILRDDTSSFISTDFLF"
     gene            5787..6068
                     /locus_tag="PKK76_05010"
     CDS             5787..6068
                     /locus_tag="PKK76_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67859.1"
                     /translation="METESTEALRESLRRFLEVHCKTKILTADPGQLSMYILHAKNQA
                     SKPRDKTILYLLLRLQEKIVDQSPDEKARVIGEFLISELNAFYKQLKTK"
     gene            6080..6841
                     /locus_tag="PKK76_05015"
     CDS             6080..6841
                     /locus_tag="PKK76_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL67860.1"
                     /translation="MDDNKTVLVVDDDPLIRDLARKILEKHYYRTLAASSVAAATKII
                     DAHRPDLILLDVHLASDDGVVFLSRLKSNPILHTVPVIMLTGDAGRDLVARAMRAGAA
                     DFLIKPIKSEMLVSRIARAMQIGHLERMKDSSGAGNVQLERQAGTTRILFTGTCDLGA
                     IEKLKAILTQTVRMQIKNDDIVVDLRFQVIENEMHIKIVRAIIEMLKPSVPRIVAGRN
                     YGALLALDVDFETQVFLSPDDVVQFLQLKHGPRKP"
     gene            6972..7211
                     /locus_tag="PKK76_05020"
     CDS             6972..7211
                     /locus_tag="PKK76_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67861.1"
                     /translation="MIERNIQDLYNAALGFLKTSEEGVKNAIANMERVFEEFKAKGSS
                     DKSEQVIRGRELIRDLSLKLEEARDYVAKLGGTQK"
     gene            complement(7266..8144)
                     /locus_tag="PKK76_05025"
     CDS             complement(7266..8144)
                     /locus_tag="PKK76_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNL67862.1"
                     /translation="MDITLMEMKSSSQFVLITGVSTGIGFGAAKHLIARGYHVIGSVR
                     NAADGARVSAELGSKFSPLVFDVTDDGAVLRAAADCEKITQGQGLAGLINNAGIAKGG
                     PLLHMPIEEIRENFEVNVIGMFRVTQAFAPQLGARKDHPSLPGRILNISSVGGKMAAP
                     FIGPYVGTKHAVEGMSHSLRRELLPYGIDVIIIGPGSVQTPIWGKRPPADRYANTDYA
                     KPLQNFLSYMEKEASEGLTIDEIGEQIASIFVNPKPKTRYALLKGKFSKWILPRLFPD
                     RMVDRLLGKGTGLLKS"
     gene            complement(8182..8667)
                     /locus_tag="PKK76_05030"
     CDS             complement(8182..8667)
                     /locus_tag="PKK76_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002107662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNL67863.1"
                     /translation="MRKIELNPATDLVLDRTVDVSPELVWKAWTVPKHLMPWFCPLPW
                     KTIECEIDLRPGGKFYTVMQSPEGQKFPGTGCYLEVTPNERLIWTSALGPDYRPNEAV
                     ENHPTFTAILTLEKKGSGTRYLVQALHSSAAIREMHEKMGFEQGWGIALDQMVAYLKS
                     I"
     gene            complement(8654..>8923)
                     /locus_tag="PKK76_05035"
     CDS             complement(8654..>8923)
                     /locus_tag="PKK76_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNL67864.1"
                     /translation="RSVSELARPFEMALPSFIQHLSVLEDAKWIKTEKQGRVRTCTLN
                     PDALALAGGWLDEQRSLWERRLDQLDDYLLKMKKTEKKEKKNAKD"
BASE COUNT         2276 a         2395 c         2177 g         2075 t
ORIGIN      
        1 gaggaatccg ctttttctaa catccatgtc cctttctcct ctacttcggg tagaccgaac
       61 cattagtcag tatcggtctg cccaaaaaga aacttttacg ctcgtgctgc gagaattcgt
      121 gttttttctc acaggcgtcc agattttttc gactctgtac gattttcggg caaaggcagg
      181 ccaacattga aagatccagc atatattcta gagggcagaa gactgcctgt gggagccttc
      241 ctgggcagtc tttcgcgact gggcccggtg gaactgggtg tcctggcggc gcaggcggcc
      301 attgaggctg cttccgtcga tcctggggct gtatcttcca ttatctgcgg caatgtagtg
      361 ccctcggggc ccgaatctct ctacctggcg cgccacgtcg ggctcaaagc aggcctgccc
      421 atctccgcat ctgctctcaa tgtgaataga ctctgcggaa gcggccttga ggcgatcgcc
      481 caggcagccc atcagatccg cgcaggagaa gatctatctc tggcgtgcgg tgtagaaagc
      541 atgagtcagt ctccttatct ggcagccggg gcaagaacgg gaatccgtta cggagacggg
      601 aaactcatcg atctattgtc cagcgctttg acagacccat acattggcca ggcaatggga
      661 aggactgcgg aaacactggc ccaggaatac gggatctctc ggcaggacca ggacgcctgg
      721 gcactcgtct cacaaacgcg agcagaggca gcacagaaat caggtaagct ttcagacgaa
      781 atcaaaagcg tccaggtcaa agacggtaaa gaaatgaaag tatttgatcg cgatgaattt
      841 gttcgcggca ccgcggcggg cgcgaagctg gccgggcttg cacctgcatt tctcgaaaac
      901 ggaaccgtca cagctggaaa cgcctccggc atcaacgatg gcgcttccgc agttgtagtc
      961 tgctctgaac gttatttgca atcgtccgga aaaaagccac tggcgaaaat tcttggatgg
     1021 gcatcgcgcg ggtgccctcc ggaaaaaatg ggaattggcc cggtgcacgc cattccggcc
     1081 gcacttgcaa gcgcgggcat ccagcaggaa caaatccaac tctgggaaat caacgaagcg
     1141 ttcgcggctc aatacctcgc ggttgcaaaa tcattgaaac tggatccgga aattacaaac
     1201 gtaaacggcg gtgccatcgc gattggccat ccactcggag catcaggaaa tagaattcta
     1261 cttggactgg caaaggaact gcgcaggcga aaactgaaat acggatgtgc ctccctttgc
     1321 attggaggag gccaggggat tgccatggtg atcgaatcgg tttagaaaat catccgaaca
     1381 aatcagagga gagatacctg tctccgcgat cacaaaggat cgtgacgatc actccatctt
     1441 ctaactgctc tgcgagtcgc gacgcggcgc agatagctcc gcctgaactc atgccgcaga
     1501 atacaccgtc cctcttcgca agaaggcgag tggttgcttc tgcgtcttgt tgagtgacat
     1561 ccattacgcg atccacacgc gccggctcaa agatggccgg taagtattcg acgggccaac
     1621 gacgaatgcc tggaatcttc gcaccatccg ccggttgaac accaatgatt tgaatggcag
     1681 ggttcttctc tttcaaaaag cgagaagtcc ccatgatggt tcctgtagtt cccatggaag
     1741 aaacaaaatg cgtcactctt ccctctgtat ctctccagat ttcaggaccg gtggtgtgat
     1801 agtgtgattt ccagttgtca gggtttgcga actggttggg catccagaaa ccaccttccg
     1861 cgaagcgctc gtgagcaaga tcgatggcgc cttccataga tctggatgca tcggtgagtg
     1921 tgactgtagc accgtatgct tgcatggcaa gtaccctctc tcgcgttgca tcctcaggca
     1981 tgatcaattc aattccgtaa cctttgacgg ctgccaccat tgccagcgcg atccctgtat
     2041 ttccgctagt cgcttccacg atccgcattc cgggcttcaa ttctcctcga agctccgcct
     2101 gttccatcat ccatagcgca ggcctgtcct tcacgcttcc ggcgggattg ttgccctcca
     2161 gctttccgaa cagacggacg cgagaccctt tccttgagag gtccacgaga ggtgtatttc
     2221 ctatcagttg actcaggcga aatgccttat ccatcgcggc ctcctttcag atcgaccctt
     2281 cgttgccgcc taacggtgaa ttcagtcaaa tgatttttca aacatttcga gttttgattg
     2341 attggcgcgg gcggaaatcg aatcatgatc ttatcaaaaa atcagaggtt atcatgagaa
     2401 aactctttgc aatcaccccc tttttgctcc tggcagctct tgcctgtagc aaaccatctt
     2461 cgtcgtccgg cggagcagaa gactacaaag cggaatcaga caagatcaag tcccagatgt
     2521 gcggaaagat ggaagaatgc atgcaagaaa tgatgaaggg catgcctgag aacatgcgtc
     2581 agatgatgtc tgcgaagttc tccaaagaat cctgtatggc gcaggcctct gccgttccgc
     2641 agaaagacaa accagaaaac ttcacaaaag aagacatgga agccgctaaa gcatgcgggg
     2701 aagccatggc aaaagcaagc tgcacagata tcaagaacca caatgtggcg ggctgcgaaa
     2761 aattcatgaa caaatgagtt ctatagttgc atccgtcgcc aaaggcggat gcaactcttg
     2821 cccctgtatt actaaagaat tttaagtatt gagacaagca aggccagctg ggccaccatc
     2881 attcccatca tccatcgcag agtctccatc cgggacgcgg atatctccaa tttcagttct
     2941 tgcttcacat cctggaaatc tctcctgacg acaagcaggt cctctttcgt tgccagctga
     3001 ctctgcacga tttctcccat ggccatagcg atctcgcgcg caacaggttc ggaagcgcgg
     3061 gcgcgcttca gcctgcggta gataccaaga gtatcaaaca tttcaggttt atccgttgct
     3121 ttcttttctg gcgaggtcaa catattttct aacatatttc ctgacggtag ctggttccaa
     3181 aatcgttccg agttcaaggc agcggaatgg atccagtctc accttcgatt tccccaaatg
     3241 ttccggaagc ccacgatcgc tttgcttctt ctatgcgatc tcgcgaagaa gaaacaaaat
     3301 tccaccacat atggcgaggg ccgtccaggg gcgcaccgcc gagcaacact gcaatcactc
     3361 catcctgcgc atccatcttc acttcctttc ccggttcaaa cacaaccaga gagtgactga
     3421 caaaattctg cccgtggatc ctcagagtgc cttctactat ataaagagcg cactctctgt
     3481 gatcctgaac aaaagtccag cttccacccc gcggaatgcg aagatccata taaaacaaat
     3541 cagaaaacgt ctgcgccggt gcagtgactc cgagtgcact tcctgccagg agagaaatct
     3601 ccactccgtc gattgtgaaa tgaggcaagt cgcgccgcgg gtgatgatga aagaaaggtt
     3661 cagacagctc ctgcgaaagt ggaagcccaa cccatgcctg cagaccgtgg attcgatacg
     3721 cgcgattcgc aagagagggt ggtcttctct ccgaatgaac gatccctttt ccagcattca
     3781 tccagtttac atctccagga tgaatttcct gaacggaacc tacgctgtct cgatgcagaa
     3841 tgcttccttc aaatagatag gtgactgtgg ccaggccaat gtgcggatgt gggcccacat
     3901 cgctcgtttc ttctggcgaa acttcccatg gacccatgtg atccagaaaa agaaaaggcc
     3961 ctactccctt tccgccatga acaggaagaa cgcgaagcac agatctttcg tctgtgagct
     4021 ggcgaacacg ggcctgcact acttctcgaa tagccatgtt caaaaaagaa aatcagtcga
     4081 aatgaaactg gacgtatcgt ctcgcagaat cgtttcaatg atggctttgt tctgagcgct
     4141 ttctttggct gcaaaaagag agcgaatcga aaatgaacga agcgcgtccg atacggaaag
     4201 agttccttct gcggatccct ttcttcccgt aaatggaaat gtatccggcc ctcgctggca
     4261 ctggctattc agattcacgc gacaaacctg attcaccagt ggatctacca tcttcgcgat
     4321 cactgcagga tcacttccga aaatgctcag ctgctggccg tagtgcgaat tgtaaacgta
     4381 gttcatggca tcgtccaggt ttgaatatgg aacaatagga atgaccggac caaactgctc
     4441 ctcatggtaa gcgcgcatgg aatcgttcac gggaaaaaga actgcaggat aaaagaacgt
     4501 cctataaaac gctccaccat tcgcattcat cactcgtgcg cccttgctct gcgcatcttt
     4561 tacaagtcca tccaggtatt cacttttccc ggggacggga agaggagtga gcttcgttcc
     4621 ggcggaccac ggcattccaa aagtcaattg agcaattttc tctgaaagcc tggttgcaaa
     4681 agtctcagca atgtcctcat gaacaaaaaa gattttgagt gccgtacatc tctgaccgtt
     4741 atacgacaat gctccggtaa ctgcctcggg aatggtcgtg tccaggtccg catctttcaa
     4801 gacgatggcc ggattctttg cacccagacc aagcacggaa cgaagtttgt gaggatgtgg
     4861 atgcgccttc ttcaaagtgt ctgcgacgcg agcagaacca atgaacgcaa tcacgtctat
     4921 cttgccgctt tccataatag ggggcacaat ttcctccccc gacccgtaca ctgtattgat
     4981 tacacccttt ggcagagaat ctcgaaatgc ctcaagaaga ggacgataca acagaattcc
     5041 aagcttgggt ggtttgaata cgcatgtatt tcccatcacg agtgccggga tcagagtcgc
     5101 gaaagtttcg tttaacgggt agttatacgg gcccatacag agcacgactc cgagtggtga
     5161 acgccgtacc tgcccgatga ttccctgttc tattagaaac ctcgacgaat ttcgatcgag
     5221 ttctttgaga gcctgaattg tatcgcgaat atagtccact gtgcgatcaa actctttctc
     5281 cgcatcttcg cgactcttcg cgatctccca catgatgaac ttcacaactt gctcgcgtgt
     5341 ggccttcatg cgcactgcga agtcttcaat gtgcttgatc ctgtgcgcga cagacatggt
     5401 aggccattcc cctcgaccca gatcataggc agacacggcc gcctccagcg cttcgagtgc
     5461 ggcctctcgc gtcattttgg gcgcatgacc gatccgatac gattcatgcg aatcgcctgc
     5521 cttcacgcag actggagagt gaatatcctg aaacgggcct ggccatgttt tcagctctcc
     5581 tgatatcaaa tattcagtct gttctacggg accgggaaag cgatcctttt caggaatctc
     5641 agataagtct ggaaacacgg aacgtagttg ttcttcgaaa gtcatgccag acattgcagg
     5701 gttcagagaa ccgcgcaatc agaaacaaac tcggacagcc ccaaaaacta cttgcgtgga
     5761 gccattgcaa gtgccgatct cttagaatgg agacagagtc cacagaagcc ctgcgtgaaa
     5821 gcctgcggcg attcctcgaa gtgcactgca aaacgaaaat actcacggca gacccgggac
     5881 aactctccat gtacatcctt catgcaaaga atcaggcgtc caagcccagg gacaaaacaa
     5941 tcctctacct tctgttgcgt ctgcaggaga agatcgtgga ccagagcccg gacgaaaagg
     6001 ccagagtcat aggggagttc ttaatttcag aattgaatgc tttctacaag cagctgaaaa
     6061 caaagtgatg agccagccga tggatgataa caagacagtt ctggtcgtag acgacgatcc
     6121 actgattcgc gaccttgcgc gcaaaatact agaaaaacat tactatcgca ctctggccgc
     6181 aagcagtgtt gccgcggcca cgaagatcat tgatgctcat cgtccggacc tgattttgct
     6241 cgacgttcat cttgcatcgg atgacggagt ggtctttcta tctcgtctga aatccaatcc
     6301 aattctacac actgtgcccg tcattatgct cacgggagat gcaggccgcg acctggtagc
     6361 gcgcgccatg cgagccggag cggccgattt tctgatcaag ccaatcaaga gcgaaatgct
     6421 tgtaagcaga atcgcgcgtg ccatgcaaat cggccacctg gaaagaatga aagattcttc
     6481 cggcgcaggg aacgttcagc tggaaagaca ggcaggcact accaggattc tattcaccgg
     6541 aacgtgcgat ctgggtgcca tcgaaaagct caaagcaatt ttaacacaaa ccgtgcgcat
     6601 gcaaatcaag aacgacgaca tagtcgtgga cctgcgattc caggtaatcg aaaacgaaat
     6661 gcacatcaaa attgtgcggg ccatcattga aatgctcaaa ccttccgtgc cgcgcattgt
     6721 cgcgggcaga aactacggcg cgctcctggc cctcgatgtg gactttgaaa cccaggtatt
     6781 cctttctccg gacgatgtgg ttcagttcct gcagctgaag catggcccca ggaagccctg
     6841 atttttcctc caactatgaa tccgatggtc cgaaccattc ggacaaaaaa tcagagaagt
     6901 acttgactgt gcaacgcacc aaggaaaaat gtgcaatgca ccaaaccatt tcggggcggt
     6961 aggagaggac aatgatcgaa agaaatatcc aggacctgta caatgctgcg ctcggtttcc
     7021 tgaaaaccag cgaagaaggc gttaaaaacg caatcgccaa catggaaagg gtcttcgaag
     7081 aattcaaagc gaaaggatcc tcagacaaaa gcgaacaggt gatccgcggc cgcgaattga
     7141 tccgcgacct cagcctgaaa ctggaagaag ctcgcgacta cgttgcgaaa ctcggcggaa
     7201 cccagaagta agacgatttg cgccgccggc gacggcggtg ctactttttt cacacgacca
     7261 tccggtcatg atttcaatag tcccgttccc tttccaagaa gcctgtccac catgcgatct
     7321 ggaaacaaac gcggcaggat ccatttggaa aactttcctt tcaagagcgc ataacgggtt
     7381 tttggttttg gatttacaaa gatactggca atctgttccc caatctcatc tatggtgagt
     7441 ccttcgctcg cttctttttc catatagctc aagaaattct gaagcggttt tgcataatct
     7501 gtgttcgcat aacgatccgc aggtggacgc tttccccaga tgggagtttg tacggaacct
     7561 ggaccaatga tgattacatc tattccgtac ggcagaagct cgcgacgcag gctatgagac
     7621 attccctcca ctgcgtgctt tgtgccaacg tatggtccaa tgaaaggcgc agccattttt
     7681 ccgcccacgg aactgatatt gagaattctt ccgggcagag aaggatgatc ctttctcgct
     7741 ccgagctgcg gggcgaatgc ctgggttacg cgaaacatac caatcacatt gacttcaaaa
     7801 ttttcgcgga tctcttcgat gggcatatgc aaaagcggac cacccttcgc gatgcctgca
     7861 ttgttgatga gacctgcaag tccctgaccc tgagtaatct tttcgcagtc agcggcggcg
     7921 cgcaagacgg caccgtcatc tgttacatca aaaaccaggg gcgaaaactt agatccaagc
     7981 tccgctgaaa cacgcgcacc gtctgcagcg tttcgcacgc ttccaatgac atgatacccg
     8041 cgcgcaataa gatgctttgc tgcgccaaaa ccgatcccgg tggacacgcc ggtgatcaac
     8101 acgaactgag aagaagattt catttccatc agcgtaatgt ccacgcgagc ggttggcgag
     8161 tgaaatacat tacaaaaagt actaaatact ttttaggtat gccaccatct gatcgagcgc
     8221 gatcccccat ccttgctcaa atcccatctt ttcatgcatt tctcgaatgg ccgcgctaga
     8281 gtgcagcgcc tgtaccagat atctggtccc gcttcccttc ttctccagag tcaagatcgc
     8341 agtgaaggta ggatggttct ctacggcttc atttggtctg taatcaggtc caagtgcaga
     8401 agtccagatc aacctttcat ttggtgtaac ctcgaggtag catcctgttc caggaaactt
     8461 ctggccctca ggcgattgca tcaccgtgta aaattttcca ccaggtctca gatcgatttc
     8521 gcactcgatt gttttccacg gcagaggaca gaaccagggc atgagatgtt tgggcacagt
     8581 ccatgctttc cataccagtt ccggggacac atccacggtg cgatcgagca cgaggtctgt
     8641 tgcggggttc aattcaatct ttcgcattct ttttctcctt cttttccgtt ttcttcattt
     8701 tcaaaagata gtcgtctaac tgatccagcc tgcgttccca gagactgcgt tgttcatcca
     8761 gccagccgcc tgccagggca agagcatccg gattgagagt gcaagtgcgc actcttccct
     8821 gcttctctgt cttaatccat ttcgcatctt caagaacaga aagatgctgg ataaaagaag
     8881 gcagagccat ttcaaatgga cgggccagct cactcacaga acg
//
LOCUS       DAORFF010000082         8871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3742_length_8871_cov_3.717559, whole genome shotgun sequence.
ACCESSION   DAORFF010000082 DAORFF010000000
VERSION     DAORFF010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3742_length_8871_cov_3.717559"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PKK76_05040"
     CDS             <1..334
                     /locus_tag="PKK76_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNL67865.1"
                     /translation="ALATADTGFAIGTGTDVAMEAADVSLMSGDLGGVADAIEASRRT
                     MRNIRQNLFWALAYNSLGIPIAAAGLLAPWVAGAAMAMSSVSVVTNALRLQRMKIGNR
                     TVRREEVS"
     gene            331..867
                     /locus_tag="PKK76_05045"
     CDS             331..867
                     /locus_tag="PKK76_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67866.1"
                     /translation="MNRLGIFVFVLILGCEHFEGELLAPKEWIEIPGLEGRWDVPHTT
                     DEPISIVKSTQAKEYRIVPRKGDVPGVVRFASIDGRIIGEYRDEKEGKPVRQGYIFRM
                     EKSGDCFLVSVGQEITEQMVNKYRLIARAEKSLKTSLPTYVLNGTAAQNRSALDALLK
                     DPHFFPERPGLKICRVAN"
     gene            complement(864..1772)
                     /locus_tag="PKK76_05050"
     CDS             complement(864..1772)
                     /locus_tag="PKK76_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNL67867.1"
                     /translation="MKIKTLILAGGTALITFAGASWYFSSVIILSQKHSVEQDRINLK
                     LNSPADFGLPQPESVVIPTRSGHISAWLFRNPSGKKCAAILHHGHTGTRWGGLKYARL
                     FFERGCAALSVDGRYHGESSGDFCTYGYYDRYDMVDVAAWLKKELVLRDSEVGMMGES
                     LGGAVSLLTAAETTLAFVAADSPFSNASAIFHEQAEKQYGPAIRVLIPVAFFFAEKRA
                     NFKIKDVSPMDAAPKITAPVFLVHSLQDDYTSPYHSELIYKAVSHDRKVLHLTDRGAK
                     HGKSIDMNYAEYKKWMDEFLSRYTHF"
     gene            1880..4258
                     /locus_tag="PKK76_05055"
     CDS             1880..4258
                     /locus_tag="PKK76_05055"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNL67868.1"
                     /translation="MRGAYRASECARMQFTNHTVGRIAPALLTLIISLYFFVRGRGQK
                     ASFWLGLYFALLSVFNIGYILGYSVDHPRGGYAWYLACAISFAAAARIQFAYSFPSEF
                     RPREKRAALILAFAASGAALVDYGLRVGSDFGFNLKHHLYGSTYSSLWVPMVSFVCFF
                     WSIVVSVRRALELLRSGRKKSGSLVFAWRNNPEFRAVCYLAGLTTMELLVNGAYLLEY
                     GRAVRTQFLPQFMNVSLLVIFGGYVLLYSTAPSTRTGFLSKLVGVCLVSVLPMIGALG
                     SAFQNMDLDRFLGERMLVGRTALQYLRQGNPVPSSVAFIVTDSDSPVYAITPGAADAA
                     RSTDVFDGRFYFAQSHSERYFSAIISDEAGHRTRAGFRYEDYRARLDESSRLIVYALV
                     MAALIIALLFPFLFRASLLMPLRLLLNDLKQIKESKVDPGLDEIGALRASFGRMLDLI
                     RETSKRIPDFAPHLNQMEDLAATEPGRIEIGGRTLIYRSRAMRRLIEQVARAREYRFP
                     VLITGETGTGKELVARMVHSDDPVPFVAVNCAALPETLWESEVFGHKKGAFTDAKSDR
                     RGRIVEAGDGVLFFDEIGEMPLAMQAKMLRLLQEGQFSPVGSDLTLTAGCRFIFATNR
                     NLADLVKEKKFREDLLYRIRVFHLEVPPLRERPEDIGDLLRFFMERFARDHKRTVPSL
                     EPAALHALVQYRWPGNIREVENAVIRAMAAGSDVLGSELFPEVGGARHASGASSGVAV
                     AIDLDSEIRRYSRSLIERALEQAKGNKTQAAELLGIKRTTLRYRMRDIGLED"
     gene            4447..5301
                     /locus_tag="PKK76_05060"
     CDS             4447..5301
                     /locus_tag="PKK76_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67869.1"
                     /translation="MKKYLTSMIALIALSGSIYAEKVIYVHGMSLTESNAHCQDTKDC
                     AYWESHVGGDYVFVGYDGRQNPFGGTDVSGSVRLLQLLNKYCRKDQGQSCRLIDDSLG
                     GFTGAGTVSMYNKTGLYNILYVTQIVSAEGGSEIANLGDTAIDILKVVFNLNGDGAQA
                     LIDSHSAIQALVTTSARALFDHNRSNGTVFYHVNANKSFFLADPFLPGTDDSLVAFHS
                     SCGYRTTGAFSKCMGEKIRSCALCFWETPKLITPFDNHKMHPLVPLTGIDIPHAQGHH
                     DVRYHGAL"
     gene            5351..6514
                     /locus_tag="PKK76_05065"
     CDS             5351..6514
                     /locus_tag="PKK76_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67870.1"
                     /translation="MNRKYFTIFIIVAVFLTVGLALLLIPDNKQSRHGSDSARSLSDP
                     SRDMGADPAAGIDPEQLIADYKEWAKYPPDSRPLLESHVDVIDFRKIPNTVQRMPTRD
                     GDKLRESGFSCLLQPEKHTATEGENMRIFLSCTHTGRPERESIRIKEIKLEAVAGSRR
                     FMPIPPTVSDAGDKGDESAGDRIYTLEFRPRQSDWADMHLTVNFLIESDTTSFTHSLA
                     THFFSSPVAPARFTGNFSEKIDHGSLVVTAELDVVKAGNYTIEANLMGDSAPVAFARQ
                     DANLRSGKQTVDLLFYGKVFHDRDVPGPYRLVGLRGSLNTDVIQPEDLARSPQEVERF
                     LSQIRSDRPMRMVIPYYDKEYKTARYSLDVFTDREYDSPGKQQRIADLSALRR"
     gene            6570..>8871
                     /locus_tag="PKK76_05070"
     CDS             6570..>8871
                     /locus_tag="PKK76_05070"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL67871.1"
                     /translation="MTCIVCELIQTMHFEVLELSANFFDRFTPSVLTFILAAFLLSRR
                     QTAARYLGAFFLFLTVFNAGYLLGYSGIHAAAPYGFHLACFITIALVFKIQFAYRMPI
                     WIYRRESIVVFVVTMLASIAAIVEYSLSAESAGYRLIFDQHIFASRYASTYIPLGSMI
                     MFFWVAIVLLRQIDHLIPSPPWFEFSSLSPWFKWPLRVLLRPVIVLSKLFRAGSAQTQ
                     AVRSFFLLVLAELTINIIVLVGFLTSFVSKELVALLLNTGLLFVYFWYGMVYINHSRE
                     STSMMVRLVGIALVTLLVVTGLIGSRANQRTESAFDGGRKSALPGLALDLGERSFADT
                     RIPGDLQYVAYRPHPSRYSRDYRIIFAKSSLSSSAMADSDVEAREYELLRRANQLKRE
                     KGTSISAARELASNEMDRQIVELDHRLYRTAAGVPYLFYVYQGKQGLYEFGFLYSDYR
                     SEMHKTVTENVVIVFAVTLVILTVFPLLFQVNLIAPLNLLLAGVARVEKGDMEHEVPV
                     QVQDEIGFLARAFNAMVKSIRDAQARLKDYADTLEIKVQDRTRELQRAYDNLDDLKRK
                     QDGDYFLTSMLIDPLARNDGASERIAVEFLIEQKKKFEFKKWKSEIGGDMNIADRIVL
                     RGKPYTMVLNADAMGKSIQGGGGILVLGSLIHAILNRTKSGAAISDYTPERWLHQTFL
                     EMDSVYRSFNASMLVSLVLFLIDEQSGLAYYLAAEHPLPVLLRAGQASFLEYPHALNK
                     LGTPGVDVAAFIETIQLRVGDRIILGS"
BASE COUNT         2003 a         2126 c         2357 g         2385 t
ORIGIN      
        1 agcgcttgca acggcagata ccggttttgc gattggcact ggaacggatg tggccatgga
       61 agcagcggat gtgagtctga tgagcggtga tctgggtgga gtggcggatg caatcgaagc
      121 gagccgcaga accatgcgca atatacggca gaacttgttc tgggcactcg cctataattc
      181 gctcgggatt ccgattgcgg ctgcgggttt gctcgctccc tgggttgccg gcgctgcgat
      241 ggccatgagt tccgtctccg ttgtgacaaa tgcacttcgg ctgcaaagga tgaagatcgg
      301 aaacagaaca gtccgtcgcg aagaggtttc atgaacaggc tcggcatatt cgttttcgtt
      361 ttgattcttg gctgcgaaca ttttgaagga gagttattgg ctccaaagga gtggattgaa
      421 atcccgggcc tcgaaggtcg ctgggatgtc cctcatacta cagacgaacc catttccatc
      481 gtgaagagca ctcaggcgaa agagtatcga attgtgcctc gtaagggcga tgtgcctggg
      541 gtagttcgtt ttgcatccat tgatggccgg atcattggag agtatcggga tgaaaaagaa
      601 ggcaagccag tccgccaggg ttatatcttt cgcatggaga agtcaggcga ttgctttctg
      661 gtttctgttg gtcaggaaat cacagaacaa atggttaata aatatagact aattgctcgt
      721 gccgaaaagt cgctcaagac cagccttcca acgtatgtgc ttaatgggac ggcggctcag
      781 aatcgtagtg ccctggatgc actcttgaaa gatcctcatt tttttccgga acgcccgggc
      841 ttgaagatct gtcgcgtcgc aaactagaag tgcgtgtagc gcgagagaaa ctcgtccatc
      901 catttcttat attcagcata gttcatatca atggatttgc cgtgttttgc gccgcggtct
      961 gttaagtgaa gaaccttgcg atcatgggag actgccttgt agatcaattc tgaatggtag
     1021 ggacttgtgt aatcgtcctg aagtgaatga accaggaaaa cgggtgcagt aatttttggg
     1081 gcagcatcca tcggggatac atctttgatc ttgaagtttg cccgcttctc tgcgaagaaa
     1141 aatgcaaccg gaatgagtac tcgaattgcc ggtccatatt gtttctccgc ctgttcgtga
     1201 aagatcgcac tcgcgttgct aaacggagaa tcggctgcga caaatgcaag agtggtctct
     1261 gctgccgtca gcaggctgac cgctccgccc agggattcac ccatcattcc gacttccgaa
     1321 tctcgtagaa ccagttcctt cttcaaccac gcggctacgt ctaccatgtc atagcgatcg
     1381 tagtatccgt acgtgcaaaa atcgccactg gattctccat ggtatctgcc atccacggac
     1441 agagcagcac aacccctctc aaaaaatagt ctagcatact tcaatcctcc ccatcgcgta
     1501 cctgtatgtc cgtggtgaag gatagctgcg cattttttcc cggagggatt gcgaaacaac
     1561 catgcagaga tatgaccgga tctagtagga atgacaacag actccggttg ggggagtcca
     1621 aaatctgccg gagaattgag tttcagattg atgcgatctt gctcaacgga gtgtttttgc
     1681 gaaagaatga tcacggatga aaaataccag ctcgcacctg caaacgtgat cagagcggta
     1741 cccccggcga gtatcagggt tttgattttc attttcgcaa actctgcgac cggacgtttg
     1801 cggcaatccc aaattgaacc tgtgcctgtg gtcgcataac ggttttcggt cacaaaattt
     1861 gccctgaacg gaaatagaat tgcgaggagc gtatcgcgcg agcgaatgcg ccaggatgca
     1921 gtttaccaat cacactgtcg gcagaattgc accggccctc ctcacgttga tcatttcttt
     1981 gtacttcttc gtgcgaggga gaggtcagaa agcatccttc tggctggggt tgtattttgc
     2041 acttcttagc gtgtttaaca taggttatat tctgggatac tccgtggatc acccgcgtgg
     2101 aggctatgcc tggtatcttg cctgtgccat ttcatttgcc gccgctgcgc gcatccaatt
     2161 cgcatacagc tttccgtccg aatttagacc gcgcgaaaag cgcgcagctc ttatcctcgc
     2221 cttcgcggca tcaggggcag cactcgttga ctacgggctg agagttgggt cggactttgg
     2281 tttcaatctg aagcatcacc tgtacggatc cacgtattcc tcactctggg tgcccatggt
     2341 cagttttgtt tgtttcttct ggagcattgt agtatccgtg cgtcgcgcac tggagctgct
     2401 tcgatccggt cgcaagaaat ccggatccct ggttttcgca tggaggaaca atcctgagtt
     2461 tcgcgcagtc tgctatctgg cggggctcac aaccatggaa ttgctcgtga atggcgccta
     2521 tctgttggaa tacggacgag ctgttcgcac ccagttcctg ccacagttta tgaacgtttc
     2581 gcttctcgtg atttttggag gttacgtcct actctattcg actgcaccat ctacgcgaac
     2641 tggctttctt tcaaaactgg ttggcgtttg tctggtttct gtgcttccga tgattggcgc
     2701 acttggatct gcttttcaga atatggatct ggatcgattt ctaggcgagc gaatgctcgt
     2761 cggtcgaacg gcacttcagt atctgcgcca gggaaatcca gtaccttctt ctgtggcttt
     2821 tattgtaaca gattctgatt ctccagtcta tgcgatcact ccaggtgcgg cggatgcagc
     2881 tcggtccacg gatgtattcg atggacgttt ctatttcgca cagtcgcatt ctgaacgata
     2941 tttttcagca atcatttccg atgaggcagg tcacaggacg cgggccggtt ttagatatga
     3001 agattaccga gcgagactcg atgagtcctc gcgattgatt gtgtacgccc tggtgatggc
     3061 agcactcatc attgcgctgt tgtttccgtt tctgtttcgc gcgagtttgc tcatgccgct
     3121 tcgcctgctt ctaaacgatc ttaaacaaat taaagaatca aaagtggatc caggcctgga
     3181 tgaaatcggc gcactgcgtg cttcgttcgg caggatgttg gatttaattc gtgaaaccag
     3241 taagcggatt ccggatttcg cgccgcacct gaatcaaatg gaagacctgg ccgccacaga
     3301 gccgggtcgc attgaaattg gagggcgcac gctcatctat cgatcacgtg cgatgcgaag
     3361 gttgatcgag caggtcgcgc gggcccggga gtatcgcttt cctgttttga tcacgggtga
     3421 aacaggcact ggcaaggaac tcgtcgcgcg catggttcat tcagacgatc cggtgccgtt
     3481 tgtagcagtg aattgtgcgg cattgccgga gacactctgg gaatcggagg tgttcggaca
     3541 caagaaggga gcattcacag atgcaaagtc cgatcgtcgc ggacgaattg tggaagcagg
     3601 agacggggtt ttattttttg atgagattgg agagatgcct ctggccatgc aggcgaagat
     3661 gcttcgtctt cttcaggaag gtcagttttc tcctgttggt tcagacctta cactcacagc
     3721 cggctgccgg ttcattttcg caaccaacag aaatctagca gatctagtca aagagaagaa
     3781 gtttcgggaa gatctgctgt atcgcattcg cgtgtttcac ctcgaagttc ctccgttgag
     3841 agagcgccct gaagacatag gtgatttgtt gcgtttcttt atggaacgat tcgcgcgaga
     3901 tcacaaacgg actgttccct ctctggagcc agctgctttg catgctctgg ttcaatacag
     3961 gtggccgggc aacattcgcg aggttgaaaa tgcggtgatt cgtgccatgg ccgcggggag
     4021 tgatgtcctt ggttccgagt tatttccaga agtgggggga gcccgtcatg cgagtggtgc
     4081 gtcgagtggg gtggccgtgg ccattgatct ggattccgaa attcgaagat actcacgttc
     4141 tttgatcgaa agggctcttg agcaggcgaa ggggaataag acgcaggcag cggagctcct
     4201 cggaatcaag cgcacaacac tgcgctatcg catgcgcgac attggcctcg aggattgatt
     4261 tctctttcga tccaatccgg cggtggcaaa tttttgccgg tgacaaaatt ctgccagtaa
     4321 cggtttctta aattagccac aaaaccagtg tttcaagaca aatgacctca cttaactccc
     4381 tggtacgcga cctgcaatag gttccccgtg agcctagcgg ctcaacaaac aaaacagggg
     4441 gagagaatga aaaagtacct cacgagcatg attgctctga ttgctttgtc aggttccatt
     4501 tacgcggaga aagtgatcta cgttcatgga atgagcctga ctgagtcaaa cgcacattgc
     4561 caggacacaa aggattgcgc atactgggag tcgcacgtcg gcggagatta cgtcttcgta
     4621 ggatatgatg gacgtcagaa cccgttcggt ggaaccgatg tgagcggaag cgtacgtttg
     4681 ctccagcttc tcaacaaata ttgccgcaag gatcaaggcc agagctgcag gttgatcgat
     4741 gattcacttg gtggtttcac cggagccggc actgtgtcca tgtacaacaa gacaggattg
     4801 tacaacatac tgtatgtgac tcaaattgtt tctgcggaag gcggttccga aatcgccaat
     4861 ctcggagaca ctgcgattga tatactcaaa gtagttttca atctgaatgg tgatggagcg
     4921 caagctctga ttgactctca cagtgcaatt caggctctcg taacaacgag tgctcgtgca
     4981 ttgttcgatc acaatcgttc caacggcact gtcttctacc atgtaaacgc aaacaaatcg
     5041 ttcttccttg cagatccatt tctgcctggc acagacgatt ctctcgttgc gttccacagt
     5101 tcatgcgggt acaggacaac cggtgcgttc agcaagtgta tgggagaaaa gattcgctcc
     5161 tgcgcacttt gtttctggga aactccgaaa ctcatcacgc cgtttgacaa tcacaagatg
     5221 catccgctgg ttccgctcac tggaattgac attcctcatg cgcagggcca tcacgacgtc
     5281 cgttatcacg gcgcgctttg atttctcttt gagccggttc gccggctcaa tttttttgtg
     5341 ggggatggtt atgaatcgta aatactttac aatctttatt attgtcgcag tttttttgac
     5401 cgttggtctt gcgcttcttc tgattccgga caacaagcag agcagacacg gcagtgattc
     5461 tgcccgatct ctttccgatc caagtcggga catgggtgcg gatccagccg ctggaattga
     5521 tcctgaacag ttgattgcag actacaagga gtgggcaaaa tacccgcctg attcgcggcc
     5581 tctactggaa tcgcacgtgg acgtcattga tttcagaaaa atcccgaaca cagtccagcg
     5641 catgcccacc agagatggcg ataagcttcg cgagtccggc ttttcgtgtc tgctgcaacc
     5701 ggagaagcac acggccacag agggagagaa catgcgcatc tttctgtcct gcacgcatac
     5761 aggcaggcct gagcgcgaaa gtatccgtat taaggaaatc aagcttgagg cggttgcagg
     5821 ttcgcgtcgc ttcatgccga ttccgccaac agtgagtgat gcgggtgata aaggggatga
     5881 gtccgcgggg gatcgtatct atactctgga gtttcgtccg cgtcagagcg attgggcaga
     5941 catgcatttg accgtgaact ttttgattga gtcggatact acgagcttca ctcattctct
     6001 ggccacgcac tttttttcgt cacctgtagc gcctgccaga ttcactggaa atttttcaga
     6061 gaagatcgat catggatctc tggtggtgac cgcggagctc gatgtggtga aggctggaaa
     6121 ctatacgatc gaagcgaatt tgatgggaga ttctgccccg gttgcattcg cgcgtcagga
     6181 cgccaatctt cgttctggaa aacaaactgt ggatttgctt ttttatggaa aggtgtttca
     6241 cgaccgcgat gttccagggc cctatcgact ggttggtttg cgtggatcgc tgaatacaga
     6301 cgtgattcag ccggaggatc tagcccgatc tcctcaagaa gtagaacgat tcttgagcca
     6361 gattcgcagc gatcgcccta tgcggatggt cattccttat tacgacaaag aatataaaac
     6421 agcccgctat tcgctcgacg tatttacgga ccgcgaatac gattcgcccg ggaagcagca
     6481 gagaattgca gatctatctg cgctgcgtcg ctgagtcggg accggcatct ttttttgaaa
     6541 cgaaggtgcc ggttgtgctc cagatatgat tgacgtgcat tgtatgcgaa ctcattcaaa
     6601 cgatgcattt tgaagtgctt gagctgagtg cgaacttctt tgatcgcttc actccttccg
     6661 tacttacctt tatacttgcc gcgtttctgc tttctcgccg tcagactgct gcgcgctatc
     6721 tgggtgcgtt tttccttttt ttgactgtct ttaatgctgg ttatttgctg ggatattccg
     6781 gcattcatgc ggccgctccc tacgggtttc acctggcgtg cttcatcacc atcgcactgg
     6841 tcttcaagat tcagtttgct tatcgcatgc cgatctggat ctatcgccgc gaatccattg
     6901 tggtttttgt tgttacgatg cttgcctcaa tagcggctat cgttgaatac tcgctgtccg
     6961 cggagtccgc tggctataga ttgatctttg accagcatat tttcgcttcg cgatacgctt
     7021 ctacttatat cccgctcgga agcatgatca tgttcttctg ggtggcgatc gttttactaa
     7081 gacaaataga ccatttgatt ccgtcgcctc catggtttga gttttcgtct ctttctccgt
     7141 ggttcaaatg gccgctgcgt gtcttgcttc gtccagtaat tgtcttgagt aagttgtttc
     7201 gagcgggatc agcacaaact caggctgttc gatccttctt cctcctggtg cttgcggaac
     7261 tcacgatcaa catcattgta ctggtaggtt ttctgacatc ctttgtttct aaggagctcg
     7321 tggcgctgct tctgaatacc gggctgctct tcgtgtattt ctggtacgga atggtctaca
     7381 tcaaccattc acgtgaaagc acgtcgatga tggtcaggct ggttggcatt gctctcgtga
     7441 ccttgcttgt tgtgacagga ttgattggat ccagagccaa ccagagaaca gagagtgctt
     7501 ttgacggagg gcggaagagt gcgcttcctg gccttgctct tgatctgggg gagaggagtt
     7561 ttgcggatac gcgcattccg ggagatcttc agtacgtcgc ctatagacct catccttcca
     7621 ggtattcgcg cgattatcgc attatttttg caaagtcttc tttgtcctcc tccgccatgg
     7681 cggattcgga tgtggaggcg cgcgagtacg aactcctccg cagagccaac cagctgaaga
     7741 gagaaaaagg aacatctatt tctgcggctc gcgaacttgc ctccaatgaa atggatcgcc
     7801 agattgtgga acttgatcat cgtctttata gaacggcggc aggtgtcccc tatcttttct
     7861 atgtgtacca ggggaagcaa ggcctctacg agtttggatt cttgtatagc gactatcgtt
     7921 ccgagatgca taagacggtg actgagaacg tcgtgatcgt gtttgccgtg acactcgtga
     7981 ttcttactgt gtttcctctt ttgtttcagg tgaacttgat cgcgcctttg aatttacttc
     8041 tggcgggagt cgcccgggta gagaagggcg atatggaaca tgaggttccc gttcaggtgc
     8101 aggatgaaat cggatttcta gcacgggcct tcaatgcaat ggtgaaatcc attcgagacg
     8161 ctcaggccag actcaaagat tacgcggaca ctcttgaaat caaagtgcag gatcgaacca
     8221 gggagctgca gcgcgcgtat gacaatctcg atgatctaaa acgcaaacag gatggggact
     8281 attttctcac ttctatgttg attgatcctc tcgcgcgcaa cgatggagcg agcgagagga
     8341 ttgcggtgga gtttctgata gaacagaaaa agaaattcga attcaagaaa tggaaatctg
     8401 aaattggcgg agatatgaac atcgcggatc gcatagttct tcgcggaaaa ccatacacta
     8461 tggttttgaa tgcggatgct atgggaaaat ccatccaggg aggtggaggg attcttgtac
     8521 tcggaagtct aatacacgcg attctgaatc gcacaaagag cggtgccgct atctctgatt
     8581 acacacctga aagatggctc catcagacct tcctggaaat ggactctgtt tatcgcagct
     8641 tcaacgcgag tatgcttgtt tctctggtct tgttcctgat cgatgaacaa agtgggctcg
     8701 cctattatct cgcagcagaa catcctcttc ctgttctact ccgcgcggga caggcatcgt
     8761 ttctggaata ccctcatgca ctgaacaagc tgggaactcc tggagtggat gttgccgcgt
     8821 tcattgaaac aatccaactc agagtcggtg atcgcatcat ccttggatcg g
//
LOCUS       DAORFF010000083         8848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3760_length_8848_cov_3.813374, whole genome shotgun sequence.
ACCESSION   DAORFF010000083 DAORFF010000000
VERSION     DAORFF010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8848
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3760_length_8848_cov_3.813374"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PKK76_05075"
     CDS             complement(<1..373)
                     /locus_tag="PKK76_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNL67872.1"
                     /translation="MFRKTRFGIAISGGGCKAFFGLGVGAVLRDAGLNLVSYSGASAG
                     SAMAASLFARNYRDVVRYFRSVTLRNPSNFHFRNLFLGARPFPHERIYRRAVRTYMDW
                     RVFVESKERIAINALEVPHELY"
     gene            372..1088
                     /locus_tag="PKK76_05080"
     CDS             372..1088
                     /locus_tag="PKK76_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNL67873.1"
                     /translation="MLLAIPVYNEKKTIPSLIKQTLANLPEQVHRVVCIDDGSTDGSA
                     EVLDEEARRHSNIEIVHQAQNGGYGRSILTSLEIGRARGYTHVITMDCDLQHRPEDLI
                     RFVQEPLDIDLVSGSRYMPESQSQGHAPGDRMEINGRITALLNSRYNWNLTDSFCGFK
                     RFRLAKIDPSVFSETGYAFPMEFWAYARAQKLTIHEIPVSRIYTTDNRTFGEDLDRKR
                     RRYRYYLETLHRSERKFKLQ"
     gene            1085..2047
                     /locus_tag="PKK76_05085"
     CDS             1085..2047
                     /locus_tag="PKK76_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67874.1"
                     /translation="MKRFQFKTYDEAVIAQVRQAVDSCLSQNGLEMSRHIIETVLSEL
                     IQNAVKANLKRVFFTSRQNNFKSDSHASEQFGEALAADAGALLSLLKNAPLSVRVEIF
                     AKQKVEIHVFNSTQMNASETKRVRQALQGKRVKGEGAGIGLGMCARLLDGIGIKKGLS
                     FQSNKNGTLFRLKIARGITAKDPRKSRVERFVQSCLPLPVAKTTADQIRGGAAPEALP
                     DAAMQFNLLHCGEKTPRTSRIKTTSNPASLEILGAMQAGEWEGIERYFLTTLSPGRLQ
                     ELRAISGRGKASRRAGIEATVAISPAVLRSILLHTDRAGTSRRK"
     gene            2084..2410
                     /locus_tag="PKK76_05090"
     CDS             2084..2410
                     /locus_tag="PKK76_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL67875.1"
                     /translation="MNISTRKKGNHLIVALAGELNLYNVGEVKNQITTTIGSDTSSLV
                     MDFKDLKYIDSSGIGLMAQLKKKYSAGSGKFALMGVSKEVMDVLRITALDSFFTFISS
                     EDKLEG"
     gene            complement(2432..3400)
                     /locus_tag="PKK76_05095"
     CDS             complement(2432..3400)
                     /locus_tag="PKK76_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67876.1"
                     /translation="MKRALVASVLLASATLHADVLRLRSGESVEGFYQSEESGIISFV
                     KITGEKMTIPSEEVENLEFSQSGIPICYTLKTSAERRCEALLGSVSSDGFVIAEGKSK
                     RSVRKIPLDDMLSYQLRKVADYQKIGPYLALDKKAVLVTNTGEKLAGRVLETVPDGVI
                     VRDAGGSNHTVQESSIASADVNMVTVPFHWFDLNNVIVGRPQMQSGRVWMGRSLMGGF
                     IGMWTGFVYEYYLASSMTKKAKGDPTVLLFNNQSYFKEFQGHQNGQRYFAAGALAIYA
                     FHWFDLYRHRDSVTSIDVQIAEAPLPDLSVRQRDPWSLALVWSYRY"
     gene            complement(3397..4932)
                     /locus_tag="PKK76_05100"
     CDS             complement(3397..4932)
                     /locus_tag="PKK76_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67877.1"
                     /translation="MSQSRILFPLILGLALLFALITQSIISVTMLELRVQIASERILN
                     FELSSRMLKTRFRQMLSGNENLKSEIKLNVLESSVLNFDKFELDGTDSWLNRFGVVVI
                     NGVRRLNLKPPIRIQEDREFMTKLQYAFYMERNRRYDVAFKQYEDMLNSGDNSQNDTR
                     GFLKLHSGYSAALTGRKDVAVSRLEQVIQEYAGTHYAETATVILDILREQGRAAEKIE
                     ASGMSDRERAAAYFRAGNFGEALTRFKKLEDLSLDEKYMLSRSYEETGNVKEAISGYI
                     DVVNRGGSSDPAVKSNRRLLLIGNFYNGGKEVREFSQQNATRLGDQEVLRNVEEGKEL
                     QLKPVIVEKILKSNDQKQSALVEEIKKELDEGTDGSSRVAVARPALPDETGALRVSVP
                     DRPDPGAITLERPSPPAIFVAQARLKVRFKDGRVVSGTRIAINKDVASVYSGDFEISV
                     PISNMEKIDAADGASALGVTPAAGNGFDTSTIRVEKEEMKFKRGGADESLPVSAIKEI
                     KAK"
     gene            complement(4929..6431)
                     /locus_tag="PKK76_05105"
     CDS             complement(4929..6431)
                     /locus_tag="PKK76_05105"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL67878.1"
                     /translation="MKKESKSRRSLRVQIGLLYTLLAFVNIIFFSVMIFSNQRDLLIL
                     NFRYQADNLVKSVQERLEKESVPLEPNGAFEDFKAALKANEITDFTIYDDKGVIWHSA
                     PDTSQRGKQVPDELKRKSIELSADLTLYRSSYLKTINEQDFTVSFLLPLRGEGDKRLY
                     LSTILSLQAIKQALRGIYIQIAIAVVWGIVFHVLFAIFLFNLIFRRVEVLTQASNEMA
                     AGNLKSRAQWKTDRNDELDNLGHAFNSMAANIEEKVETISKLNEEIQTELRIGKEVQK
                     QFIRGTDIIPKYKGQIYYRPMREVSGDIYGMFAFPKGFNGFFFADASGHGVSAALITT
                     ITIQNLENAARKFMNPRKIVSTLNNSLAARFESSYFATGTFFLFHPNGETLITNAGHL
                     PILCLRPSTGDILEIAKMGPPMGLMEDFEYPVKRLIGQPGDRLLVYSDGLMETNNVEK
                     EQFSLARIKELFLQHSKNSTEEFLADLVQQFETWAAEYRDDVSAIVLEVP"
     gene            complement(6489..7538)
                     /locus_tag="PKK76_05110"
     CDS             complement(6489..7538)
                     /locus_tag="PKK76_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67879.1"
                     /translation="MRVLSSIILFALSATVAVAEDGKCVAGDCTKGSGTAIWGADVYT
                     GSFQNGKPGGPGTFKLADGRTFSGNFTNGEATGQFQVNYPDGSIYNGELVGGRRKGKG
                     TLKSMRGIYRGEFDQDKIQGQGTFDFASGEVYKGEFKDGQMHGQGTMKFRDGTLYKGE
                     FKDGKMAGPAHVEYPDGRIYDGEFTGGHMQGTGKLALKDGAVYEGDFANGQMHGQGTI
                     RFANGDKYSGEMDHGKIQGRGKLEYHEGAVYEGEFKSGLPHGEGELSSPRGIKYKGEF
                     KNGQMEGKGEIFYKSGSRYVGQVKDGKKEGEGEWTLSNGTVLKGRFKGDSYAGSAETQ
                     ESDPSKPPEKGQKGE"
     gene            complement(7618..8016)
                     /locus_tag="PKK76_05115"
     CDS             complement(7618..8016)
                     /locus_tag="PKK76_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67880.1"
                     /translation="MKGNNILLRLTIFIVPLLSSCASYNIALYDTKIPVYFTEGDTPD
                     QAGRHFKFERYLTWILFDTVKMQDLNLDEAIKAEVPTAKKIVNLRVSSSESGVDSVVR
                     FFGTLIQYLLVADRSLVSKRTVIVEGDYYE"
     gene            complement(8003..8425)
                     /locus_tag="PKK76_05120"
     CDS             complement(8003..8425)
                     /locus_tag="PKK76_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67881.1"
                     /translation="MRVVCILLLSFLFLAGCAETQYKVNSRDLVVRLNGFQKSEGFTK
                     KREFEIKFRRYYCLWGLIRLTDRTLDDLLKEETRPGDFVGNLQINEKYDFADGLVDTI
                     LYGLLRPYSVVVRGEIYMRSAVTEPAETKKVEGKDEGK"
     gene            8632..>8848
                     /locus_tag="PKK76_05125"
     CDS             8632..>8848
                     /locus_tag="PKK76_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67882.1"
                     /translation="MRVDWSNPNLLSVLAALGSVFLILMIRYFVMAGGAYALFWKILP
                     DRLRARRIQKDLPRKGHIKSEILYSIST"
BASE COUNT         2199 a         2369 c         2078 g         2202 t
ORIGIN      
        1 gatagagttc atgtggcact tccagtgcat tgatggcaat gcgttctttt gattcaacaa
       61 agacgcgcca gtccatgtag gtccgcacag ctctcctgta gattcgttcg tgagggaaag
      121 gacgcgcgcc aaggaaaaga ttacgaaaat gaaaattaga gggattgcga agagtgacag
      181 atctgaagta gcgaactaca tcgcgatagt tgcgtgcaaa caaactcgcg gccatggcgg
      241 atcctgcact tgcgccgcta tatgaaacca gattcaatcc agcatctcgt agaaccgcac
      301 ccactccgag cccaaaaaat gccttgcacc caccgccgga aatggcaatc ccaaatcgcg
      361 tctttcgaaa catgcttcta gccatcccgg tctataacga aaaaaaaaca attccatctt
      421 tgatcaagca gactcttgcc aacctgcctg agcaagtcca ccgggttgtg tgcattgacg
      481 acggctccac ggacggatcg gcggaagttc tcgatgaaga agcgcgacgc cacagcaata
      541 tagaaatcgt ccaccaggct cagaatggag gatacggcag aagcattctg acatcgctcg
      601 agattggacg agcccgaggt tacactcacg tcatcacaat ggactgcgat ctgcaacaca
      661 gaccggaaga tttgattcga tttgtgcaag aacccctgga catcgacctg gtaagcggct
      721 ccagatatat gcccgaatcg caatctcagg gacatgctcc gggcgatcga atggaaatca
      781 acgggcgcat cactgcattg ctcaactcgc gctacaactg gaatctcaca gattcgttct
      841 gcggtttcaa gcgatttcgc ctcgcgaaga tcgatccttc tgttttttcg gagaccggtt
      901 acgccttccc catggagttc tgggcctacg cgcgggcgca gaagctgacc atccacgaga
      961 ttcctgtgag cagaatctat actacagaca acagaacttt cggggaggac ctcgatcgca
     1021 agaggagaag ataccgatac tatctagaga cgctgcatcg ctctgaaaga aagttcaaac
     1081 tacagtgaaa cgattccaat tcaaaacata cgatgaagca gtcatcgcac aggtgcgcca
     1141 ggcagtggat tcgtgcctca gccagaacgg cctcgagatg tccaggcaca ttatagaaac
     1201 agtattgagc gaactcatcc agaacgcagt gaaagcgaat ctaaaaagag tcttctttac
     1261 ttccaggcaa aacaacttca aaagcgattc gcacgcttca gagcaatttg gcgaagctct
     1321 ggcagcggac gccggagctc tactgagtct tctgaagaac gcacctctca gcgtgcgagt
     1381 cgagattttc gcaaagcaaa aagtggagat ccatgttttt aacagcacgc agatgaacgc
     1441 ctccgagaca aagagagtgc gtcaggcatt gcagggaaaa agagtaaaag gcgaaggcgc
     1501 ggggatcggt cttggaatgt gcgctcgcct gctcgatggc attggaatca aaaaagggct
     1561 gagctttcag tcgaataaga atggaactct ctttcgattg aaaatcgcgc gcggaatcac
     1621 ggcaaaagac ccgcgaaagt ccagggtaga aagatttgtg caatcctgtt tgccgttgcc
     1681 agtcgcgaaa accacggcag atcaaattcg cggtggagcg gcgccggaag ctctaccgga
     1741 tgcggccatg caattcaacc tgcttcactg cggagaaaaa acccctcgca cttccaggat
     1801 caaaactact tcaaatcctg cgtcgcttga aattctggga gccatgcaag ccggagaatg
     1861 ggaaggaatt gaacggtact ttttgaccac tctgtctccc ggtcggttgc aagaactcag
     1921 ggcgatcagc ggaagaggca aagcgtccag gcgggccgga attgaggcaa ctgtggcaat
     1981 ttcacccgcc gttctgaggt ccattctact ccatacagat agagcaggca cctcccgtag
     2041 aaaatgattg acactgacct tcctggaaag gaactttcga cagatgaaca tttccacacg
     2101 gaagaaaggc aatcatttga tcgtcgctct ggcaggagag ctgaacctgt acaacgtggg
     2161 cgaagtgaaa aaccagatca ccacaaccat cggctcggat acgagttctc tggtcatgga
     2221 tttcaaagat ctcaaataca ttgattcttc tggaattggc ctcatggctc agctcaagaa
     2281 gaaatacagc gcaggcagcg ggaaattcgc acttatggga gtgagcaagg aagtcatgga
     2341 tgttttgcgc atcactgctc ttgacagctt ctttacattc atcagctcag aagacaaact
     2401 ggaaggctga tcttcgcctt cgcgcgacca atcaatacct gtaggaccag accagagcca
     2461 gactccaggg gtctctctgt ctcacggaca aatcagggag cggagcttcc gcaatctgta
     2521 catctatact ggtaacagaa tctctgtgtc tgtataaatc aaaccagtga aacgcgtaga
     2581 tggccagtgc gccggctgca aagtatctct gtccattctg atggccctga aactctttga
     2641 aataagactg attgttgaag agtagaaccg tgggatcccc tttcgccttc ttagtcatcg
     2701 agctggccag atagtattcg tacacaaagc cagtccacat tccaatgaaa ccgcccatca
     2761 acgatcgccc catccagacc cggccacttt gcatttgtgg tctgcccaca attacgttgt
     2821 tcaaatcaaa ccagtgaaaa ggtactgtga ccatgtttac atctgcgctt gcaatcgaac
     2881 tctcttgcac agtatgattg gatccacctg catctcgcac gatgactccg tccggaaccg
     2941 tctctaaaac tcttccggct aacttctcgc ccgtgttcgt taccagcact gccttcttgt
     3001 cgagtgccag atacggacct attttctggt agtccgctac cttgcgaagc tgataggaaa
     3061 gcatgtcatc cagaggaatc tttcgcacgc ttcgtttgga ctttccttct gcgatgacaa
     3121 agccgtcaga agaaacactg ccgagcaaag cttcgcatct tctttcagcg ctcgttttta
     3181 gagtgtaaca gatgggaatg cccgattgcg aaaactcgag attctccact tcttcagatg
     3241 gaatggtcat cttctccccg gtgatcttca caaagctaat gattccggat tcttctgatt
     3301 gataaaagcc ttctacggat tcaccgctac gaagacgcaa aacgtccgca tgcaacgtcg
     3361 cagatgcaag caaaacagat gcgacaagcg cacgtttcat tttgccttaa tttccttgat
     3421 cgcagaaact ggaagggatt cgtccgcgcc tccgcgcttg aatttcattt cttctttctc
     3481 cacgcgaatc gtagaagtat caaaaccatt tccggcggca ggggtcactc cgagcgcaga
     3541 cgctccgtca gcagcatcaa tcttttccat gttcgaaatc gggacggaga tttcaaaatc
     3601 accggaatac acactggcca catccttgtt gatggcaatg cgcgtaccgc tgaccacgcg
     3661 accatctttg aatcgcactt tcagtcttgc ctgtgcaaca aaaatggctg gaggagaagg
     3721 tcgctccagt gtaatggcgc ctggatcagg acggtccggc acggacacgc gaagagcgcc
     3781 tgtttcatcc ggcaatgcag gacgagccac cgccacgcga gaggagccgt ctgtgccctc
     3841 atccagttct tttttgatct cttccaccag cgcgctctgc ttctggtcat tagacttcag
     3901 aatcttctct acgattacag gcttgagctg caattccttg ccctcttcca cgtttcgcag
     3961 cacttcctgg tctcccagac gcgtggcgtt ttgctgcgaa aattcgcgca cttcctttcc
     4021 accgttgtaa aaattgccaa tcagaagcaa tcgccgattg gacttcactg caggatcact
     4081 gcttccaccg cgattgacca cgtcgatgta tccgctgatt gcttctttta catttccagt
     4141 ctcttcatag gagcgcgaaa gcatgtattt ctcatccaga ctgagatctt ccagcttctt
     4201 gaatcgagtc agagcctctc caaagtttcc agcgcgaaag taagccgcgg ccctctcgcg
     4261 atcagacatt ccggatgctt caatcttttc cgccgcgcgc ccttgctcgc gaagaatatc
     4321 cagaatcact gtggctgttt ccgcataatg agttccggca tattcctgaa tcacctgttc
     4381 gagtcttgaa accgccacgt cttttctgcc ggtgagcgcc gctgaataac cggaatgaag
     4441 cttgagaaat ccgcgagtgt cgttctggct attgtccccg gaattcagca tgtcttcgta
     4501 ttgtttgaag gctacatcgt atctacgatt tcgctccata taaaacgcat actggagctt
     4561 tgtcatgaac tcgcggtctt cctgaatgcg aatgggcggc ttcaaattca atcgccgcac
     4621 gccgttaatg acgactactc caaatcgatt caaccagctg tccgttccat ctagctcaaa
     4681 tttgtcaaag ttcagaacac tggattcaag cacgttgagt ttgatttcgg acttcaggtt
     4741 ctcattcccg gatagcatct gtcgaaagcg cgtcttgagc attcgagaag aaagctcgaa
     4801 attgagaatt cgctcgcttg caatctgcac gcgcagctcg agcatcgtta cgctgatgat
     4861 ggactgggta atgagcgcaa acaatagcgc aagtcccagg atcagcggaa acagaatgcg
     4921 gctctggctc acggcacctc cagcacaatg gcggagacat catcccggta ttcagcagcc
     4981 catgtttcaa actgttgaac cagatctgcg agaaattcct ctgttgaatt cttagaatgc
     5041 tgcaaaaaca gttccttgat tcgagcaagg gaaaactgct ctttctccac gttgtttgtt
     5101 tccatcagac cgtctgagta cacaagcagg cgatccccgg gctgaccgat caagcgcttc
     5161 acaggatatt caaaatcttc cataagaccc atgggtgggc ccatcttcgc aatctccagt
     5221 atatccccgg tagaaggacg cagacagaga atcggaagat gtcctgcatt tgtgatcagt
     5281 gtttctccat tgggatgaaa caggaaaaaa gttccagtgg caaaatacga agactcaaag
     5341 cgcgcagcca gactgttgtt gagagtggaa acaatcttgc gcgggttcat gaattttcgc
     5401 gcagcattct ccaggttttg aatggtaatg gtagtaatga gagccgcgga tactccgtga
     5461 cccgatgcat ccgcaaaaaa gaatccgtta aaccctttgg ggaacgcaaa cattccgtag
     5521 atatcgccgc tcacttcgcg catagggcga tagtaaatct ggcctttgta ttttggaatg
     5581 atgtctgtcc cgcgaatgaa ctgcttttgc acttccttac caatgcgaag ctcagtctgg
     5641 atttcttcat tgagcttgga gatcgtctcc accttttcct caatgttggc ggccatagag
     5701 ttgaaggcgt ggcccaggtt gtctaactca tcgtttcgat ccgtcttcca ttgagcgcgc
     5761 gacttaagat ttccggcggc catctcgttg gatgcctgag tgagcacttc cactctgcgg
     5821 aaaatcaaat tgaatagaaa gatagcgaat aacacatgga agacgatgcc ccatactact
     5881 gcgattgcaa tctgaatgta aattccgcga agagcctgct tgattgcctg aagactgagg
     5941 atggtggaaa gatagagtct cttatctcct tcgccgcgga gcggaagcaa gaacgacaca
     6001 gtgaaatcct gttcgttgat tgtcttgaga taactggaac gatacagagt cagatccgca
     6061 gaaagctcaa tggactttcg ctttaattca tctggaacct gtttgcctct ctgcgatgta
     6121 tccggcgcac tgtgccagat gactcccttg tcgtcgtaga tggtgaagtc tgtaatttcg
     6181 tttgctttga gcgcggcttt gaagtcctca aacgcaccgt tcggttccag tggaacgctt
     6241 tctttttcga gtctctcctg aactgatttc accaggttgt ccgcctggta gcgaaagttc
     6301 agaatcagca agtctctctg gttcgagaag atcatcacgg agaagaagat gatgttcacg
     6361 aacgcaagaa gcgtatacaa tagaccgatc tggacccgga gagatctgcg acttttggac
     6421 tcttttttca tggccttatc ttctaagatc ggcctttcag gccgttttcc ggtgcaccgg
     6481 cggcctaact actcaccttt ttggcccttt tccgggggtt tagatgggtc cgattcctgc
     6541 gtctcggcac ttccagcgta gctgtctccc ttgaatctgc ccttgagaac agtgccgttg
     6601 ctcagcgtcc attccccttc tccttccttc ttcccgtcct ttacctggcc tacgtatctc
     6661 gatccactct tatagaagat ttctccctta ccttccatct ggccgttttt gaattctccc
     6721 ttgtatttga ttccacgggg agaagagagt tcgccttcgc cgtgaggcag accggacttg
     6781 aactccccct cgtaaactgc tccctcatga tattccagtt tccctcgccc ctggatctta
     6841 ccgtgatcca tttcaccgga gtacttatct ccgtttgcga agcgaatggt gccctggccg
     6901 tgcatctggc cgtttgcgaa atctccctca tagacagctc cgtctttcaa ggcgagcttg
     6961 cccgtgccct gcatatgtcc gccagtgaat tctccgtcat agatcctacc atccggatac
     7021 tccacgtgcg ccgggccggc catctttccg tccttgaatt cccctttgta tagagtgccg
     7081 tcgcggaatt tcatggttcc ctggccgtgc atctggccgt ccttaaattc ccctttgtac
     7141 acttccccgc ttgcgaaatc aaacgtgccc tggccctgga ttttgtcctg gtcaaactct
     7201 ccgcggtaga tgccccgcat gcttttgaga gtgccttttc cttttctgcg gccgccgacg
     7261 agttcgccat tgtagatgga accgtccggg tagtttacct gaaactggcc cgtggcttcc
     7321 ccgtttgtga aattaccaga aaaggttctt ccatccgcga gtttgaatgt acccggacct
     7381 ccgggcttgc cgttctgaaa cgatccggta tagacgtccg cgccccagat ggcggttcca
     7441 ctgcctttcg tgcaatcccc tgccacacac tttccgtcct cagccacggc taccgtggca
     7501 gacagggcaa acaaaatgat ggagctcagc actctcacaa ttcaataaga cgatcgggcg
     7561 cgcactctgt ccacacgaaa gcgataggat tctcccgact gcggtgacac gaatgcatca
     7621 ttcgtaatag tcaccttcca cgattacagt ccgcttcgag acaagagaac gatcagccac
     7681 tagcagatat tgaatcagag tcccaaaaaa gcggacgacg ctatccactc cggattcaga
     7741 agaactcacg cgcaggttca cgattttttt tgccgtgggg acttccgctt tgatggcttc
     7801 gtctagattt aagtcttgca tcttgactgt gtcgaacagg atccacgtga ggtatctctc
     7861 aaacttaaag tggcggccgg cttgatctgg cgtgtcaccc tcggtgaaat agacagggat
     7921 tttggtgtcg tagagtgcaa tattgtaaga cgcacaactc gaaagcaacg gcacgataaa
     7981 aatcgtcagc ctcagaagta tgttatttcc cttcatcctt gccctccact ttttttgttt
     8041 cagccggctc tgtgacggcg ctccgcatgt agatctctcc gcgcacaact acggagtacg
     8101 gccgcagcag cccgtaaaga attgtatcca ccagtccatc cgcaaaatcg tatttttcat
     8161 tgatctgaag gtttccgaca aagtcgcctg gtcgcgtttc ctccttgagt aaatcatcca
     8221 gcgttcgatc tgtgagtcga atgagacccc agaggcaata gtagcgtcgg aatttgattt
     8281 caaactcccg cttcttcgta aaaccttcgc tcttttgaaa accattgagg cgaaccacga
     8341 gatcccgact gttgactttg tactgggttt cagcgcaacc ggccaggaac aaaaagctga
     8401 gtaatagtat acaaacgact ctcacaaaat tcctccgtcc ccgcaaaata gcatgcatgt
     8461 atcaagaacc ccggtcaaat agaaattagc cgcctggcaa tttattcgag cttatgttga
     8521 aaatgggaac cagattcaac cataacaatt gatgtcccct tccacggctg cctttgagct
     8581 tgcaggcaag aaggatgaaa ccgactctgg atccagaaag gtctaataat tatgcgtgta
     8641 gactggtcca atccgaatct tttatccgtg ctggctgctc ttggatccgt gtttttgatc
     8701 ctgatgattc gctactttgt catggccggc ggggcgtatg ctctgttctg gaaaatactt
     8761 ccagatcgac ttcgcgctcg tcgcatccaa aaagaccttc cgcgaaaagg acacatcaaa
     8821 agcgaaatac tgtactccat ttctacat
//
LOCUS       DAORFF010000084         8706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3850_length_8706_cov_3.628367, whole genome shotgun sequence.
ACCESSION   DAORFF010000084 DAORFF010000000
VERSION     DAORFF010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8706
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3850_length_8706_cov_3.628367"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..2018
                     /locus_tag="PKK76_05130"
     CDS             147..2018
                     /locus_tag="PKK76_05130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNL67883.1"
                     /translation="MPGHNSIQRAAFAALAPFGLSEFRPGQLEAIEGILSGKDVMVIL
                     PTGGGKSLIYQLPAVMRETGLTLVISPLIALMKDQADSMRAIGVAAEYCNSTQDELEQ
                     RRVLSHCVTGKVKILYVSPERALSGDFLAILPRMNVNLIAVDEAHCISQWGHDFRPEY
                     RALHKLRIPGVPVAALTATATARVTRDVISSLDLHDPEIIKKSFFRPNLHYRVLHPEN
                     DREKQRILLDLLEERKFRENAPGKCIIYCATRKKVDQVFEFLGSYGFRVGRYHAGRTS
                     AGREKMQDAYSAGKVNVLVATNAFGMGMDALDVRLVLHYQVPSSLDAYYQESGRAGRD
                     GQVSDCILFFSNADFVTQSFILGRSAGEDLLAPVRDFGYSQMCRQQYICSYFGEEISS
                     CGVCDCCLEDGSASREDFLERERKSQTHRDARASYEFSDSEIESVHAVLRDYPGKFGK
                     KILAGVLRGSKARDIIRYRLTQSSAYGALKDVPEEAIIRFFQDGIDNGIIKIEGIKYP
                     KIYLAQEPPRARVRKESPRKSGPTEASDLLKKLKSFRDREARRLKWKKYMVMQNGLLS
                     RIALSRPSSFAELARIKGMGDSRAEKYGPEILRIISESLYSAGVQGKSIHGTSRNQS"
     gene            1993..3597
                     /locus_tag="PKK76_05135"
     CDS             1993..3597
                     /locus_tag="PKK76_05135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNL67884.1"
                     /translation="MGLRATNLEKLDKMTFDVLVLGGGINGAISAAVLAARGARVALI
                     DRADFASFTSQESSNLVWGGIKYMETLEFGLVRNLCMSRNHLIRSYPSTVQEIRFFVN
                     LEKGFRWPRLFLFMGSLLYWVIGNFFTKGPRLLSKADIEREEPVVRQDNSMGGFEYSD
                     AYLHDNDARFVFGFVRTALDNGAIAANYVESLGSKKDGQFWNTQARDVLTGRTFDIKS
                     RLIVNACGPYVEEQNRLTDVKTSHRHVLSKGIHLLINRVSPSKKVLTFFASDGRLFFV
                     IPMGTRSCIGTTDTRVDKLPAVVTEEDREFVLKNINHLLKFDKPITKADVIAERCGVR
                     PLVVNPASEKKDKGDWTSLSRKHAVEMQKGLGHISVFGGKLTDCLNIGEEIAELVSEC
                     GVKLPYPDAIWYGEPPLEIRQRFFHQAKLMDLDSMTAVDSSEPLSARLWRRYGTSAFA
                     LLEDIRDNPQNAEVLIEGTEYIRCEIHMAARREMVTKLDDFLRRRSKIALIEKKETIA
                     RAPGIREACKILFGEDADARYAEYFG"
     gene            3671..4510
                     /locus_tag="PKK76_05140"
     CDS             3671..4510
                     /locus_tag="PKK76_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67885.1"
                     /translation="MDYLIRITETDSLTPGDVIRRVGDGKEQQGCFVRRDESGGLIAG
                     DVVDLVNFSFVTEVGMLRSGSDFEFFKHKHRFSTSSVAEAARAIITGWVLFKEHLDLQ
                     KSILSFVESVYSPEHIMELSRDARLQALFVPVQQKFKIGKFKEKIDWEKVRDQRFRDA
                     LEALVGGKHMTYLAFIPGEQSHKPVFYTIGTKPHQETNVSLQREPFAFKPTHGGHIKI
                     VSDESDKVKRFIVDAGSNFIGVGSHTSLATAEMITNALHELFPQHEFTALAGRGAHGA
                     QSY"
     gene            complement(4507..4773)
                     /locus_tag="PKK76_05145"
     CDS             complement(4507..4773)
                     /locus_tag="PKK76_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67886.1"
                     /translation="MSETLCDFCNEVQIYTGTNATTNYLPDALRSVMYRKNLKSGEIL
                     RCPACNRAYFFDERTPGGSDDAMTTTFYQDFYPLTAEELSALER"
     gene            complement(4770..6554)
                     /locus_tag="PKK76_05150"
     CDS             complement(4770..6554)
                     /locus_tag="PKK76_05150"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNL67887.1"
                     /translation="MITTEGLAYLTPNLAAILLTLALCIRCWKSQSLPGHKELAFALI
                     AELLWMCGHIGKLTSPTLEGKIIWDDIEFLGTCAWPMATFAFALKFSGARLPRILHAG
                     LWILPLFIAGAALTDPWTAYLHPNARLVNTTPFTGLLYDFKLILSVTFLQILLQVTLA
                     AVLIIARSFWLTTSQRVQSMILSAGILLPVIGSLLILLGIQFTEHRDTSHITFGVGNV
                     LIYFGLFGISKEAEHRFSTLFDNASDAILIMNGLMIIDCNIKAQTIFQASKEKLVGTS
                     VIDACAPVQEERTCEPLWRANVEAAMSGQPQFFGWTFVRKTGEEFDAEVSLTCTQVGP
                     DMLLQAFVRDRSEIRAAALERESLIRDLRAKNIELERFNYSVSHDLKSPLITIKGFVG
                     MLAQDVEKQNYANVPNHIHRISSAADKMYSLLEDLLALSRAGKATSAPALNEMDEILD
                     ESLGALEGLIQSRSAEIIRKERLPLIFADAMRMKAVWQNLIENSIKYARHGVPPKIQI
                     GCKADAQGYAFYVQDNGQGIDPRYQLQVFGQFHKLDASTEGSGIGLSLVQRIMEAHQG
                     RAWVESDGVGRGATFWFHLPRTPKASDG"
     gene            6734..7435
                     /locus_tag="PKK76_05155"
     CDS             6734..7435
                     /locus_tag="PKK76_05155"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNL67888.1"
                     /translation="MKKRLPTLTASILGLLIIAGLTAEEKRISHADVARSVMAVYEKM
                     GSYSASFQITSQDGNNARAMSGQCFYKGPGKIRFDFDNPAGNLIVSDGRTMWVYIRRL
                     NAVGKQDLRLQKKDESGKNIFQDTAGPGVSRLFRKYHYRFDRPEQPRNEDGSSVFVLD
                     MDQREKTGGYEKIKLYVDASSYLIRKAVASDSQGKSTTLVFRNPALNPPLEGKLFQYD
                     PPESVRVVPNPLVSE"
     gene            7440..8522
                     /locus_tag="PKK76_05160"
     CDS             7440..8522
                     /locus_tag="PKK76_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNL67889.1"
                     /translation="MLAKRLGTILKDAREAKKLSIKDVARETFITARWVEALENEDFS
                     VFPAETYALGALKSYSEFLNLETSHLENLFRGQQIDLSQSPIEELTRPTGIRMPAIGK
                     NVVFVIAGLAVGLGLLLLVMSGALKFSSGTGSSSAHSSEANCHDREIIPVTLPARGAP
                     PRQEAVSRENAIRFNVDSTALKICLGEVQQTPEGKARALFLMRINDESDFSFYAAEGE
                     MVALSSDTQALKELSGEIRITPKAIGEASARIQLESGEKGAQPQGAIQVTLQFVADSY
                     VEWTDDGNYHRGVEIRAGESRTFEAKSRLEVKVGNGAGVQVIAPGKPPRIAGPPGKIV
                     KITYRKVPDPLDPGLAQIEEKIEAAQ"
     gene            8519..>8706
                     /locus_tag="PKK76_05165"
     CDS             8519..>8706
                     /locus_tag="PKK76_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNL67890.1"
                     /translation="MKSPSYYITTLGCPKNQADSREMERSLSQEGYIRTDSADQADVH
                     LINSCAFIEDARVETIRTV"
BASE COUNT         2208 a         2099 c         2289 g         2110 t
ORIGIN      
        1 tccctggcgc gtgggttgat tcagaagttg ttgagctaaa cgacgcctaa cgtctgaggc
       61 cgcgccaaag acgcggcatg ctttctttga agcatacagg aagaaaacag aattgactaa
      121 catgcgtata gtgttattct ggacagatgc cgggacataa ttctatccag cgcgcagcgt
      181 tcgccgcgct cgccccgttc gggctaagtg aatttcgtcc gggccagctg gaagcaatcg
      241 aaggaatcct ctcgggaaaa gacgtcatgg tcattctgcc gaccggcggc ggaaaatctc
      301 tgatctacca gcttccagct gttatgcgag aaaccggtct gactctggtc atttcgccgc
      361 tgattgcgct catgaaggac caggcggatt ctatgcgggc tatcggcgtt gccgcggagt
      421 actgcaattc cacacaggac gaactcgagc agaggcgagt gctcagccat tgcgttaccg
      481 ggaaagttaa gattctttat gtttcgccgg agcgcgcttt atctggagat tttcttgcca
      541 tccttccacg catgaatgtg aatctcattg cagtagatga agctcattgc atctcgcagt
      601 ggggacacga tttcaggccg gagtatcgtg ctcttcataa gctgagaatt cctggtgttc
      661 ctgtagccgc actcactgca actgcaacgg cgcgcgtaac cagagatgta atttcgtctc
      721 tggatcttca tgatccagag atcatcaaga aaagtttttt tcgacctaac cttcactacc
      781 gtgttttgca tccagaaaat gatcgtgaga agcaaagaat cctgctcgat ctactcgaag
      841 aaaggaagtt tagagagaat gcgccgggca agtgcatcat ctattgcgct actcgaaaga
      901 aagtggacca ggtattcgaa ttccttggtt cctatggctt tcgcgtcggt cgctaccacg
      961 ctggcagaac cagtgccggt cgtgagaaaa tgcaggatgc ttattccgcg gggaaagtga
     1021 acgtacttgt ggccacgaac gcatttggaa tggggatgga tgcgctggat gttcgcctgg
     1081 tcctgcacta tcaggtgcct tcttctctgg acgcgtacta tcaggaatcc ggccgggcgg
     1141 gaagagacgg gcaggtttca gattgtatac ttttctttag taatgctgat tttgtaacgc
     1201 agagtttcat cctgggccgg agcgcgggag aagatcttct ggcacctgtt cgtgactttg
     1261 gttattcgca gatgtgcaga caacagtaca tttgttccta ctttggagaa gaaatttctt
     1321 cctgcggtgt ctgcgattgt tgcctggaag acggctctgc cagccgcgaa gattttctag
     1381 aaagagaaag aaagagtcaa acacaccgcg acgcgcgtgc ttcttacgag ttcagcgatt
     1441 ctgaaattga atccgtacat gcggtgttgc gcgactatcc aggaaaattt ggaaagaaaa
     1501 tactggccgg cgtgcttcgc ggaagcaaag cacgcgacat cattcgctat cgcttgactc
     1561 agtccagtgc ctatggagca ctcaaggatg ttccggaaga agccatcatt cgtttctttc
     1621 aggatggaat cgacaacggg attataaaaa tagaagggat caagtatcca aagatttacc
     1681 tggcccagga gccgccccgt gccagagtca ggaaggaatc gcccagaaag tcaggtccaa
     1741 cggaagcatc cgacttgctc aagaagttga aatctttcag ggacagggaa gctcgtcgtt
     1801 tgaaatggaa gaaatacatg gtcatgcaga acggtttgct ttcgcgcatc gctctgtccc
     1861 ggccgtcttc atttgctgag ctcgctcgca tcaagggaat gggggattcg agagcagaga
     1921 aatatggccc tgaaattcta cgcatcataa gcgaatcgct ttactccgct ggagtccagg
     1981 gaaaatcgat tcatgggact tcgcgcaacc aatcttgaaa aactcgataa gatgacgttc
     2041 gatgttctcg ttctgggtgg cgggatcaac ggggcaattt ctgcggcagt tctggctgcc
     2101 aggggtgcgc gagttgcgct catagacaga gcagacttcg catctttcac cagtcaggaa
     2161 tcgtccaatc tggtctgggg tggaatcaaa tacatggaga ccctggagtt cgggctcgtg
     2221 cgcaatctgt gcatgtctcg caatcatctg attcgcagtt atccgtccac ggtgcaggaa
     2281 attcgattct ttgtgaatct ggagaagggg tttcgctggc ccagactatt tctgttcatg
     2341 ggttcgcttc tttactgggt gattggaaat ttcttcacga aaggcccgcg tcttctttca
     2401 aaagcggaca ttgaaagaga agaacccgtg gtgcgccagg acaacagcat gggcggattt
     2461 gaatattcgg atgcatacct tcacgacaac gatgctcgct ttgtttttgg ttttgtacgc
     2521 acggctctgg acaacggtgc cattgcggcc aactatgtgg agtcccttgg atcaaaaaag
     2581 gacggccaat tctggaacac gcaagcgcgc gacgtgctca ccggacgcac attcgacatt
     2641 aaatctagat tgatcgtcaa tgcatgcggt ccgtacgtag aagagcaaaa tcgcctaacg
     2701 gatgtaaaga ccagccatcg ccacgttctc tccaaaggaa ttcatcttct catcaatcgc
     2761 gtcagcccca gcaaaaaagt tctcaccttc ttcgcaagcg atgggcgcct cttctttgta
     2821 attccaatgg gcactcgctc ttgcattgga acaacagaca ctcgtgtgga caagcttcct
     2881 gcagttgtca cagaggaaga cagagaattc gtactaaaga atatcaacca tttacttaag
     2941 tttgacaagc caatcacaaa ggcagatgtg attgcggaaa gatgtggcgt tcgtcctctg
     3001 gttgtgaacc cggcttctga aaagaaagac aaaggggact ggaccagcct ctcgcgaaag
     3061 catgcagtgg aaatgcagaa gggtctcggg catatcagcg tctttggcgg aaagctcacg
     3121 gactgcctga acataggaga agagatcgcc gaactggttt cggaatgtgg agtcaagctt
     3181 ccctatccgg acgccatctg gtacggtgag cctcctcttg aaatccgcca gcgtttcttt
     3241 catcaggcca agctcatgga tctggattct atgactgcgg tggattcctc agaacctctg
     3301 agcgcccggc tctggagacg atacggcaca agtgcgtttg ccctcctcga agacattcga
     3361 gacaatccgc agaatgcaga agtccttata gaaggaaccg agtacattcg ctgcgagatt
     3421 catatggcgg ccagacgcga aatggtcaca aagctagacg acttccttcg aagaagatcg
     3481 aagattgctc ttatagagaa aaaagaaaca attgcacgtg ctcctggaat tcgcgaggca
     3541 tgtaaaattc tgtttggaga ggatgcggac gcgcgttatg cggaatattt tggatgactt
     3601 tgcacgcggc gggtattttg ggtgagtaaa tttgccgcga ctgtcaggcg caaattcttg
     3661 aggatggatc atggactatc tcatcaggat cactgaaaca gactctctta ccccgggcga
     3721 cgtgattcgc agggtaggtg acggcaaaga acagcagggc tgttttgttc gccgcgatga
     3781 gtcaggtggt ctcatagcag gagacgtagt agatctggtt aatttttcgt ttgtaacaga
     3841 agtaggaatg cttcggtccg gatcggactt tgagttcttc aaacacaaac acaggttttc
     3901 aaccagctcc gttgcagagg cggcgcgcgc catcatcaca ggctgggttc tatttaagga
     3961 gcatctggat cttcagaaat ctatcctctc ttttgtagag agtgtttatt cgccggagca
     4021 tatcatggag ctcagccgtg acgcgaggct acaggcactg tttgtgcccg ttcagcagaa
     4081 gttcaagatt ggaaaattca aagaaaaaat agactgggag aaggtgcgcg atcagcgctt
     4141 ccgcgatgca ctcgaggcgc tcgtcggcgg aaagcacatg acgtaccttg cgttcattcc
     4201 aggcgaacag agtcacaagc ctgtttttta tacgatcgga accaagcctc atcaggaaac
     4261 aaacgtaagc ttacagagag agcctttcgc atttaagccg actcacggcg ggcacattaa
     4321 gatcgtaagc gatgagtctg ataaagtgaa acggttcatt gtggatgccg gctcgaattt
     4381 cattggagta ggttcgcaca cttctctggc cacagcggag atgattacaa acgctctgca
     4441 tgaattattc ccgcagcatg agttcacggc tctcgcagga cgcggcgctc atggagcgca
     4501 gagttactaa cgttccagcg cacttaattc ttccgcagtc aacggataaa aatcctggta
     4561 gaacgtagtc gtcatcgcgt cgtcgcttcc acccggagtg cgttcgtcaa aaaagtaagc
     4621 gcgattgcat gccggacaac gcaggatttc cccgctcttt agattctttc tatacatgac
     4681 agatcgtagc gcgtccggca aatagttggt ggtggcgttt gttcccgtgt agatttggac
     4741 ttcattacag aagtcgcata acgtttcgct catccgtcac tcgccttcgg ggtgcgcgga
     4801 agatgaaacc agaatgtggc acctcggcca actccatccg actctaccca cgcccgaccc
     4861 tggtgcgctt ccatgattct ttgcacgaga gagagcccga tgccagatcc ttccgtggat
     4921 gcatcgagtt tgtggaattg cccaaaaacc tgaagctggt agcgaggatc aattccctgt
     4981 ccgttgtcct gaacgtaaaa tgcatatcct tgcgcatcag ctttgcagcc gatttgaatt
     5041 ttcggtggaa ctccgtgacg tgcgtacttg atggaatttt cgatcaggtt ctgccatacc
     5101 gctttcattc tcatcgcgtc tgcgaaaatg agtggcaatc tctcttttcg aatgatttct
     5161 gcactgcgag actggatcag cccttcaagt gcgcccagcg attcgtccag aatttcgtcc
     5221 atctcattga gtgccggcgc actggttgct ttgcccgcac gggaaagtgc cagtagatct
     5281 tcgagcaggc tgtacatttt gtccgcggct gaactgattc tgtgaatatg atttggtacg
     5341 ttggcgtagt tttgtttttc aacatcctgt gccagcattc ctacaaaccc cttgatggta
     5401 atcagagggc tttttagatc gtgcgacacg gaatagttga agcgttccag ttctatgttc
     5461 tttgctctga gatctctaat caggctttcg cgttcgagcg ccgccgctct gatctcagag
     5521 cgatccctca caaaggcctg aagcaacatg tctggcccca cctgtgtgca cgttagactc
     5581 acttctgcat cgaactcttc tccggttttg cgaacaaaag tccagccaaa gaactgcggt
     5641 tggccactca tggcagcttc aacgttcgct cgccatagag gttcgcaagt cctctcttcc
     5701 tggactggtg cgcatgcatc aataacggaa gtaccaacga gtttttcttt ggatgcctga
     5761 aagatagtct gtgccttgat gttgcaatca atgatcatta atccgttcat gatcaaaata
     5821 gcatcacttg cattgtcgaa taacgtggaa aatctgtgct ctgcttcctt tgaaattccg
     5881 aacaaaccaa aatagatcag gacgttacca acgccaaaag tgatgtgcga agtgtcgcga
     5941 tgctctgtga attgaatccc gagtaaaatg agcagactgc cgatcaccgg aagcaggatg
     6001 ccggcggaaa gaatcatgga ttgcactctc tggcttgtgg tcagccagaa agatcgcgcg
     6061 atgatcagaa cggcggctaa cgttacctgc agcagtatct gaagaaatgt gacggataga
     6121 atcaatttga aatcatacag caacccggtg aacggcgtag tgttcacgag tcttgcattc
     6181 gggtgcaaat aggcagtcca cggatctgta agcgccgctc ctgcaatgaa gagaggaagg
     6241 atccagagtc cggcgtgcag gattcgaggc agtcgagctc ctgagaattt caaagcaaaa
     6301 gcaaatgtag ccatgggcca ggcgcatgtg ccaagaaact caatgtcatc ccatatgatt
     6361 ttgccttcca gagtgggcga ggtgagtttg ccaatgtgac cgcacatcca gagtagctca
     6421 gcaatgaggg caaatgccag ttccttgtgg cccggcaggg attggctttt ccaacagcga
     6481 atgcacaatg caagcgtcag taggatggcg gccaggttcg gtgtgaggta ggcaaggcct
     6541 tctgtggtga tcatctcagg actttcccgg cagaaacaag cactcgccaa aaagaagtca
     6601 agtcttcaga tttgctgcga caggccaaaa acagctttag aaaagcgttc aggcccccat
     6661 tccgctgtgt ggaggcatat gcgccgaaaa ggtcttgacc ctgggccctt ctttgagact
     6721 tctccctgtg cgcatgaaaa aacgcctgcc caccctcaca gcgagcattc tagggctcct
     6781 gatcattgcc ggcctcacag ccgaagagaa aagaatctct cacgcggacg tggccagatc
     6841 ggttatggcc gtttacgaga aaatgggatc ctacagtgcc agtttccaga tcacaagtca
     6901 ggatggaaac aatgcacgcg ctatgagcgg tcagtgcttc tacaaagggc ccggaaagat
     6961 tcgcttcgat tttgacaatc ctgcggggaa cttgattgtt tcagacggta gaaccatgtg
     7021 ggtctacatt cgaaggttga acgctgtagg gaaacaggac cttcgtcttc aaaagaaaga
     7081 tgaaagcgga aagaatattt tccaggacac agcaggtcca ggcgtttcta gattgtttag
     7141 aaaataccac tatcgttttg acaggcctga acagccgcgc aacgaagatg gaagcagtgt
     7201 ttttgttctg gatatggacc agagagaaaa aacgggggga tacgagaaga tcaagctgta
     7261 cgtggatgct tcgtcatact tgattcggaa agccgttgct tccgatagtc agggaaaatc
     7321 cacaactctc gtatttcgca atccagcatt gaatccgccg ctggaaggaa aacttttcca
     7381 gtatgatccg ccggaatccg tgcgggtcgt tcccaatccc cttgtgagtg aatgattgaa
     7441 tgttagctaa aagactgggc actattctaa aagacgcacg tgaagcaaag aagctctcca
     7501 taaaagacgt tgcacgggaa acgttcatca ccgcgcgatg ggtggaagca ctggagaatg
     7561 aagatttctc cgtcttcccg gcagagactt atgcactcgg tgctttgaaa tcgtattccg
     7621 aatttttgaa tctagaaaca tctcatctgg aaaatctttt ccgtggccag cagattgatt
     7681 tgtctcagtc tcccattgaa gaactcacca ggcctactgg gattcgtatg cctgcgatcg
     7741 gcaagaacgt ggtgtttgtc attgcaggtc ttgcggttgg tctgggtctt ctgcttctgg
     7801 tgatgtctgg cgcgctgaag ttttcgagcg gaacaggttc atcatccgca cattcctctg
     7861 aagcaaattg ccatgaccgc gaaatcatcc ctgtgactct tcctgcaaga ggggctcctc
     7921 ctcgtcagga ggcagtgtcc cgcgaaaatg caatccgctt caacgtagat agcactgctt
     7981 tgaaaatttg tctgggtgaa gtgcaacaaa ctccagaagg aaaagcacgc gcgctctttt
     8041 tgatgagaat caatgacgag tccgattttt ccttctatgc tgcggaagga gaaatggtag
     8101 cactcagttc agatacgcaa gctctcaaag agctcagcgg tgaaattcgg atcactccaa
     8161 aagcaatcgg agaagcttcc gctcgcattc aactggaatc aggcgaaaag ggagcgcaac
     8221 cgcaaggcgc cattcaggta actctgcagt tcgtcgcgga ttcatacgtg gaatggaccg
     8281 acgatggaaa ctatcaccgt ggcgtagaaa tccgcgcagg ggaatcacgc acatttgaag
     8341 caaaaagcag gctggaagtg aaagtaggaa acggagccgg agttcaggtc atcgctcctg
     8401 gcaagcctcc tcgcattgca ggtccacctg gcaaaattgt aaagattact tacagaaaag
     8461 ttcctgatcc gctcgatccg gggcttgcgc agatcgagga gaagatcgaa gcggcgcagt
     8521 gaaatctccg tcgtactaca tcacgacgct cggctgtccc aagaatcagg cagacagccg
     8581 ggaaatggag cgaagtcttt cgcaggaagg atacattcgt acggactctg ccgatcaggc
     8641 agatgtgcat ctcatcaatt cctgtgcttt cattgaagat gcaagagtgg aaaccatccg
     8701 cacagt
//
LOCUS       DAORFF010000085         8694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3859_length_8694_cov_3.772890, whole genome shotgun sequence.
ACCESSION   DAORFF010000085 DAORFF010000000
VERSION     DAORFF010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3859_length_8694_cov_3.772890"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PKK76_05170"
     CDS             complement(<1..386)
                     /locus_tag="PKK76_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67891.1"
                     /translation="MRLLNAAILAVTLHQTACITTALHTMAFPDGYDFEHAQDIHDLE
                     ASGNSFVFCAQSPRTTRQIKVTGDSSRSYAMSSETLSIFETQKVWETNITDGCKAKGP
                     TGSFETAGTLFFSTQSVSMTAMKSRPV"
     gene            473..991
                     /locus_tag="PKK76_05175"
     CDS             473..991
                     /locus_tag="PKK76_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67892.1"
                     /translation="MRNLFILAVLAFFSMCTPAKKNGGNSVDPGGKFDFQVQYLYSGE
                     TFYDTLIIRDRTMVRKYFNDDGKKCAKAQRSPCWVESELESATATLTAEELASLKSLV
                     EETNFMVSDPDPHGLTGKGRFYAYIITVNHKSVEHRVVMGEFPGSADAPPASRRIYLR
                     LLDLAKAKSLGN"
     gene            complement(1397..1819)
                     /locus_tag="PKK76_05180"
     CDS             complement(1397..1819)
                     /locus_tag="PKK76_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67893.1"
                     /translation="MNWIIDWPYKWFAPFLPERCGKSAPHWLAFVDKTWVYTDRVRLA
                     EYLAAAPVVLITDAEEGRCYFCGSDLGNASCLRSDGIWLWENGLDHYVLEHAIRLPDT
                     FVAHIEEREFRPPAEVHDDFEFLPMPTGAEVQGLLHLE"
     gene            complement(2281..2931)
                     /locus_tag="PKK76_05185"
     CDS             complement(2281..2931)
                     /locus_tag="PKK76_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67894.1"
                     /translation="MIPSEVQKFRTLYLSQEVSKYYLGLGHLVFTSTICILVVGYSIS
                     QIHNVSWLEFLTVPITFLYANIAEYFGHKGPMHHKRKFLSLIFKRHTMQHHRYFTDNA
                     MECDSFMDYKMILFPPILILFFFGFFALPVGVLLYVTLSKNVGFLFVATAIGYFLNYE
                     WLHLAYHQKEDSWVYSIPFLRALRKHHLKHHDPAIMARSNFNITYPICDTIFRTKV"
     gene            complement(2928..3728)
                     /locus_tag="PKK76_05190"
     CDS             complement(2928..3728)
                     /locus_tag="PKK76_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNL67895.1"
                     /translation="MSPLITQTHSVPGGKIFQITMNRPDVHNCVNGQMAKLFLAAWTE
                     FRDNPDLTVCVLHGAGDKSFCSGADLAALGELANINASADEIAHTVRHGTGPLGGSRI
                     LQKKPVITVSQGYTYAGGLELYCHGHIRIAEKQALFSVACRRWGVPLVDGGTVYLPRL
                     LGFAGALPLIITGQRIRADRAYQIGLAWEVVEKGKGLDRALTIARQVCAQPRDALFAD
                     MRSVLDGFHLPIEEALRMEAENLHPVMRSQSTQEGVDKFLRGERFWVK"
     gene            complement(3725..4174)
                     /locus_tag="PKK76_05195"
     CDS             complement(3725..4174)
                     /locus_tag="PKK76_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNL67896.1"
                     /translation="MLEKTKVSEILKDKGKAIVSVDPGATVFEALSLMAERNTGAVLV
                     MSEGRLEGLLSERDYARKVVLRGRNSRETHVREIMSRELIIVHPEDSVMESLAIMTEK
                     RIRHLPVMEGSEVVGLISIGDLVKRVIDEQHIMIKHLENYISGSYMA"
     gene            complement(4251..4604)
                     /locus_tag="PKK76_05200"
     CDS             complement(4251..4604)
                     /locus_tag="PKK76_05200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL67897.1"
                     /translation="MLDRLEFTTTKKDGHTIIEIRGEMDVYNSFELKNLMEELSQNGS
                     TKFILKMGALSYIDSSGLGALINQQMRLQKSGGEMFLVSLSDQVRSVLELTKMHSFFK
                     ISGSENEALSASSAA"
     gene            complement(4614..6353)
                     /locus_tag="PKK76_05205"
     CDS             complement(4614..6353)
                     /locus_tag="PKK76_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL67898.1"
                     /translation="MNYRGARMIVGGLTSKNKIDILLYISKEFAYIDNQRDLFDRVVA
                     LSVEIFEVDNVTVRLWQNGQLVPVRFLKETTPPRRPLRPGEGYSGAVFIEKKPVNLDN
                     LEHYPDYLDENESTRCAMVVPILFRDEALGTISIEKDTAYFYKKDDLEILEAMASQLA
                     LALNEVKLIEGLVEARSRIQSDLKMGRSVQSQIIPSEIAPWNSIRFGHYFEPMVEVSG
                     DYFDVMRSGNSLTCIIADVSGHGVPAALVTMALHFQFRRCVEQGFGLTEMMEELNESI
                     RHRLPDGTYFTTQLIRIYADHTYSYVNAGHVRLMHYKRAQKEFIFHDTKGIPLGIAPV
                     RRTDYEEKFSRLEPGDALVMLTDGFYEQRTATHEETGLQTVINWFGEILGAANPPDAA
                     ETTVQFLDRFNKHVEGWPREDDLTLLVLDCSVHLENARKVYLEARKFLREKNLSKAEE
                     LALEAYRLDSSLHDNLLLLSRVYNEQSRPDEAARFLSEYVVSSGAHKPEYYHMLGTLY
                     YRNGDIRAAKRELKKSLSLDHTFTRASLMLARCYLKEGARPKAIKTLQQALKSSPANE
                     QVRQSLKNVESMA"
     gene            6446..8068
                     /locus_tag="PKK76_05210"
     CDS             6446..8068
                     /locus_tag="PKK76_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNL67899.1"
                     /translation="MKTAGVLSLKILFAFIVLCMFAGVSSLFADPSGHSTGTAADAMD
                     AQGELFLPQKPAPGADPAAVSAYEDFQRKAATEPLAVKLADSVGQNRIAINLMWTLIT
                     GFLVMFMQAGFALVETGLCRAKNASHTMLMNFLIYVIGLTGFWICGFGLMFGAMPVTS
                     LGGTPGLSPEYEVTIDLFGKTFGLFAWNGFFLTGSSYDVGIYTIFLFQMVFMDTAATI
                     PTGAMAERWNLKSFYVFGFFLSMFLYPIFGNWAWGGGWLSTLGVNFGLGHGYVDFAGS
                     GVVHAVGGLCALAGAIVIGPRLGKYNADGSVNPIPAHHVPMALLGAFILAFGWFGFNP
                     GSTLGVSGGGLNRVGIVAVVTMLAGAGGALSATALIIWKAGRPDPTMVANGMLAGLVA
                     ITAPSGFVSAPIGFLIGAIAGVLVVLSVDFFDHLKIDDPVGAISVHGVCGSFGVLSVG
                     IFADGTANFGGSWNGVAGNVKGAIFGDWGQLFAQLIGVATVIVWSFGVSFIFFKILHK
                     VMGMRVSEQVELDGLDLDETGIMGYPSFPTVE"
     gene            8103..8486
                     /locus_tag="PKK76_05215"
     CDS             8103..8486
                     /locus_tag="PKK76_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNL67900.1"
                     /translation="MRQNISTGSKWEPIIGYSRCVKVGNHVYVSGTTASGPNGFVGEG
                     DPYAQTKQIIENISKALEQAGASLKDVVRTRIYMTNIDQWEIVGKAHGEFFGSIMPAT
                     AMVEVKRLISPEILVEIEADAVIQG"
     gene            8467..>8694
                     /locus_tag="PKK76_05220"
     CDS             8467..>8694
                     /locus_tag="PKK76_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67901.1"
                     /translation="MQSSRAKKSRRLLIVGGLIVAAGIGWGAFRSLNPYVGLKIYKNP
                     ARCYALSIPGHVQIQAEDPSHVTFAAADWTGG"
BASE COUNT         1973 a         2187 c         2240 g         2294 t
ORIGIN      
        1 acaggcctgg acttcattgc ggtcatggat acactttgag tggaaaaaaa tagcgtgccg
       61 gcggtttcaa aggagcctgt tggacccttc gccttgcagc cgtccgtgat gttcgtctcc
      121 cacactttct gagtttcgaa aatgctcaac gtttcggatg acattgcgta gctgcgactg
      181 gaatcgcctg ttactttgat ctgtctggtt gtgcgtggag attgagcgca gaagacgaac
      241 gaatttccgg aagcttcaag gtcgtggatg tcctgagcat gctcgaaatc gtaaccatcc
      301 ggaaatgcca tcgtgtgcaa agcggtcgtg atgcatgcag tctggtgcag tgtgaccgct
      361 agaatggcgg cattgagcag tctcatgatg tctcaacgag agtcttgctc gtgccctgtc
      421 aatgcacaat cgattgcttg cctgccgcga gtggtgtggc tttcttgcac atatgcgaaa
      481 ccttttcatc ctggcggtgc ttgctttttt ctccatgtgc actcccgcga agaagaacgg
      541 aggtaactca gtagatccgg gtgggaagtt tgattttcag gtgcagtatc tttactcagg
      601 cgaaacgttc tacgacacct tgatcattcg cgatcgtacc atggtacgca agtatttcaa
      661 cgatgacggg aagaagtgcg cgaaagcgca aagatcaccg tgctgggtgg aatcagagct
      721 cgaaagtgca acggcaactc tgactgcaga agaattggcc tcgttgaaga gtctggtaga
      781 ggaaaccaat ttcatggttt ctgatccgga cccacacggt cttactggca agggccggtt
      841 ctacgcatac atcatcacag tgaaccataa gagcgtagag catcgcgtag ttatggggga
      901 gtttccaggt tcagcagacg cgcctcctgc atcgcgacga atctatcttc gcctcctgga
      961 tcttgccaaa gcaaagtccc tcgggaatta atcgcctcgt gcggcctccg cgcggagaat
     1021 ccgccgcagg tgccgggata cagtccatcc tcgcgcgcgc tgttgcgacg ctcggacgct
     1081 ccgaacgccg ctgtggaccg caccccggca caacggcata agccgcgctg gacctgcgag
     1141 cctttcattt tgcgcgcgcg gaccacgctc cttcaggctt cgcgttcgct tcggtctcgc
     1201 tttgcgagac tgcttcgcac cttgcggatc gttggccgga agttgttcga gtgccaacaa
     1261 tgccgtgtaa cgtcaggcta agaggagcac atatccgcct gtggcggatt tgaatagatg
     1321 ggcgacgtgg agtttgttgc gaggaacata agtccttctg cgacgccgct tcatccgaaa
     1381 acttgcccga ttgtccttac tccaaatgca ataacccttg tacctcagcg ccggtcggca
     1441 tcggcagaaa ctcgaaatca tcgtgaacct cggcaggagg ccgaaactcc cgttcttcaa
     1501 tatgcgccac aaaagtatcg ggcaaccgaa tagcgtgctc cagaacgtag tgatccaatc
     1561 cgttttccca gagccaaatc ccgtcagacc tcaagcaact cgcattgcca agatcgctgc
     1621 cgcaaaaata acagcgtcct tcctctgcat ctgtaatcaa tacaacaggt gccgcagcta
     1681 gatattccgc caagcgaacg cggtccgtgt agacccacgt tttgtccacg aatgctagcc
     1741 agtggggcgc gctctttccg caccgttctg gtaagaacgg ggcaaaccat ttgtagggcc
     1801 agtctatgat ccagttcatt aatcacctac tcgggcatgg aactaatcca ctttccacgt
     1861 ttgctcagat ccagccacgg tttccctttt agtcgagttt attggcccac gtcttttcat
     1921 ttggcgccat gatgggacat tcaactggtt ggccaacttc tgcgcaaaac cattctgaag
     1981 ctttccagtc tcacatgaaa gcaattgtat cgctgttcct tcatagtcag ctcgattctt
     2041 gggtagttgc gcgaggtaca aactacggcc aaacaatgca ccgcgtatcg tcagattcca
     2101 ttgactcacg agactaccat ggacgcggga aatatgaatt cggcggtgaa ttcgaatttg
     2161 ccagttagga gagaaagtcg ttatgtttcc cagcgggaaa atgtgccctt gacttccaat
     2221 cctcgtgcgc ctcaggtttc tcgcgtcgcg gctttccagc gatttggtcg cgcctttcat
     2281 ttaaactttt gtgcgaaaga tcgtatcgca gattggatac gtaatattga agttggatct
     2341 ggccatgatc gccggatcat gatgtttcag atgatgcttt cgtagcgcgc gaagaaaagg
     2401 aatggaataa acccacgagt cttctttctg atgataggcc aggtgcaacc attcgtagtt
     2461 taagaaatat ccaatggcag tggcaacgaa tagaaatcct acgttcttcg aaagagtgac
     2521 atagagaagc actcccacgg gcagggcaaa aaatccaaag aagaacagaa tcaagatagg
     2581 tggaaacagg atcatcttgt agtccatgaa ggaatcgcat tccattgcat tgtcagtaaa
     2641 gtagcgatga tgctgcattg tgtggcgttt gaagatcagg ctcaggaact ttcgtttatg
     2701 atgcatgggc cccttgtgcc cgaaatactc tgcaatgttt gcatagagaa aggtgatggg
     2761 cactgtcagg aattcgagcc atgatacatt gtgaatctgg ctgatggaat aacccacaac
     2821 caggatgcaa atggtgctcg taaacaccag gtgacccaga ccaaggtagt atttagaaac
     2881 ttcctgagag agatagaggg ttctgaattt ttgaacttct gatggaatca tttcacccag
     2941 aacctttcgc ctcttaaaaa tttatcaacg ccttcttgtg tgctctggct tcgcataaca
     3001 gggtgaaggt tttctgcttc catgcgcaac gcttcttcta tgggcaaatg aaatccgtcg
     3061 agtacgcttc gcatatctgc aaaaagagcg tcccggggtt gagcgcaaac ctgcctggca
     3121 atcgtgagag cgcgatcgag gcctttcccc ttttctacaa cttcccaggc caggccaatc
     3181 tggtatgcac gatctgcgcg aattctctgg ccggtgatga tgagtggtag cgcccctgcg
     3241 aagccaagaa gacgcggtag ataaactgtc ccgccatcga ccaggggaac tccccacctt
     3301 ctacatgcga ctgaaaacag agcctgtttc tctgcaatgc gaatgtgacc atggcagtac
     3361 aattccagac cgcctgcgta cgtgtagccc tgcgaaactg tgatgaccgg tttcttctgt
     3421 aggattcgcg aaccgcccag aggtccagtt ccgtgccgaa ctgtgtgtgc gatttcgtct
     3481 gcgcttgcat ttatgtttgc aagttctcca agtgcagcaa gatctgcgcc ggagcagaat
     3541 gacttatcgc ctgcgccatg taagacgcac acagtaagat ccggattgtc tctaaactca
     3601 gtccaggcag caagaaacaa ttttgccatc tggccgttga cgcagttgtg cacgtccggc
     3661 cgattcatcg tgatttgaaa aatctttccg ccgggaactg aatgcgtctg cgtgatcagc
     3721 ggactcatgc catgtaggaa ccggaaatat agttctccag atgcttgatc atgatgtgct
     3781 gctcgtcgat gactcgtttg accaggtcac caatggaaat caagccaaca acctcgctgc
     3841 cttccatgac gggcaggtgg cgaattctct tttctgtcat gattgccagg gactccatca
     3901 ctgaatcttc aggatgaacg atgatcaatt cgcggctcat gatttcgcgt acatgagttt
     3961 cgcgtgaatt tcgaccgcga agcactactt ttcgcgcata atctctttcc gacaggaggc
     4021 cttcgagcct gccttcgctc atgacgagca cagcgcctgt atttctctcc gccatcaagc
     4081 tgagagcttc aaaaactgtg gcgccaggat cgacggaaac gatggctttc cctttatcct
     4141 tcagaatctc tgaaacttta gtcttctcca gcatgccaac cctccgtgcc cagtttttga
     4201 gcacaggtct gacccatttg tcagggtaag caatcaaaaa cggggcgcct tcaggcggcc
     4261 gaggaggcgg acagggcttc gttttcagaa ccggatatct tgaagaaact gtgcattttg
     4321 gtcaattcca gcacgcttcg gacctgatcg gacagactga cgaggaacat ttctcctcca
     4381 cttttttgca gtcgcatttg ctggttgatc agtgcaccca ggccgcttga atctatgtag
     4441 ctgagcgcac ccatttttaa aatgaacttt gtagatccgt tctggctaag ttcctccatc
     4501 agatttttca actcgaagga attgtaaacg tccatttctc ctcgaatttc gatgatggtg
     4561 tgaccgtctt tctttgtagt agtaaattcc agtcgatcga gcatatcccc tcctcacgcc
     4621 atactttcca cgttcttcaa tgactgccgc acctgttcgt tcgcaggtga actcttgagc
     4681 gcctgttgta gagtcttgat tgcttttggt cttgcccctt ccttcaagta gcatcgcgcg
     4741 agcatgaggc tggctctggt gaatgtatga tccagagaga gagatttttt caattctcgc
     4801 ttagcagcgc gtatgtcacc atttctatag taaagagttc cgagcatatg atagtactca
     4861 ggtttgtgtg caccactcga tactacgtat tcgctcagga atctcgcggc ttcatcgggc
     4921 ctggactgtt cattgtagac gcgcgaaagc aaaagcagat tgtcatgaag agaagaatcc
     4981 aggcgatacg cttcgagagc cagctcttcc gcctttgaga gattcttttc gcgcaagaat
     5041 tttctggcct ctaaatatac tttgcgcgcg ttttctagat gcacgctgca atcaaggaca
     5101 agcagagtga ggtcatcttc ccggggccaa ccctccacgt gtttgttgaa acggtccagg
     5161 aattgaaccg ttgtctccgc tgcatcgggt ggatttgccg cgcccagtat ttctccaaac
     5221 cagttaatga ctgtctgaag tcctgtctct tcatgagtgg cggttcgttg ttcatagaat
     5281 ccgtcggtga gcattacgag cgcgtctccg ggctccagtc tgctgaattt ctcttcgtag
     5341 tctgttcttc gcaccggagc aatgccaagt ggaatccctt tcgtgtcatg gaagatgaat
     5401 tctttttgcg ctcttttgta gtgcatcagg cgaacgtgtc cggcattcac ataagagtat
     5461 gtgtggtccg cgtaaattcg aatcaattgt gtcgtaaagt aagttccgtc cggaagccga
     5521 tgccgaatgc tttcgttcag ttcttccatc atttctgtaa ggccgaaacc ctgttccacg
     5581 catcttctaa attggaaatg aagtgccata gtgacgaggg ctgcaggaac gccgtgaccg
     5641 gaaacatccg caatgatgca ggtcagactg tttcctgatc gcataacgtc aaaatagtct
     5701 ccgctcactt ccaccattgg ttcaaagtaa tgaccgaatc gaatgctgtt ccagggcgcg
     5761 atttcagacg gaatgatctg gctttgtaca ctgcggccca tcttcaggtc gctctggata
     5821 cgtgagcgag cttccacaag gccttcgatc agtttcactt cattgagagc gagagccagc
     5881 tgggatgcca tggcttcaag aatctccagg tcatcctttt tgtagaaata cgcggtatcc
     5941 ttttcaatgg atatggtgcc cagggcttcg tcgcgaaaga ggataggaac caccatcgcg
     6001 caccttgtac tttcgttttc atccaggtaa tcggggtagt gctctagatt gtccaggttc
     6061 acaggttttt tctcaatgaa aactgctccc gaataccctt ctcctggtcg cagtggacga
     6121 cgcggcggag tggtctcttt caggaatcgc actggaacca gttgcccgtt ttgccagagt
     6181 ctgactgtta cgttgtccac ctcaaagatt tccactgaga gagcgaccac ccgatcgaac
     6241 agatcccttt gattgtcaat gtacgcgaat tctttagaaa tatatagcaa tatgtcgatt
     6301 ttgttttttg acgtaagacc gccgacgatc atgcgagcgc cgcggtagtt cacgactttc
     6361 ctcttgccat atttcaaagc gatcatgcta acccttgagc aatatctata ccagggcctg
     6421 gctcgctttt tgcaaaacgg caggtatgaa aaccgccggc gttctatctc tgaaaatact
     6481 cttcgccttc atcgttttgt gcatgtttgc gggcgtttca tcgctttttg cggatccttc
     6541 cgggcattcc acaggcacgg cggcggatgc catggatgcg caaggagaac tcttcttgcc
     6601 tcaaaaacca gcaccgggag cagacccggc tgctgtttcc gcgtacgagg actttcagcg
     6661 aaaagctgcg acagaacctc tcgccgtcaa actcgcggac tccgttggac agaatagaat
     6721 cgccatcaac ctcatgtgga ccctcatcac cggttttctg gtcatgttta tgcaggccgg
     6781 ttttgccctg gtagagacag ggctgtgtcg tgcgaagaac gcttcgcaca ccatgctcat
     6841 gaactttttg atctacgtaa ttggtctgac gggattctgg atttgcggat ttggtttgat
     6901 gttcggtgcg atgccagtta caagcctggg cggaacacct ggtctgagtc cggaatacga
     6961 agtgacgatc gatctattcg gaaagacatt cgggctcttc gcatggaatg gattttttct
     7021 gacagggtcg tcgtatgacg ttggaatcta caccatcttc ctctttcaaa tggtgttcat
     7081 ggatacagcc gccaccattc ccacgggcgc gatggcggaa agatggaatc tgaaaagttt
     7141 ctacgtcttt ggtttcttcc tctccatgtt tctctacccg atttttggaa actgggcgtg
     7201 gggaggtggc tggttgtcaa cgttaggcgt gaactttgga ctcggacatg gctatgttga
     7261 tttcgcaggt tccggagtcg tgcatgcagt cggtggtcta tgcgctctgg ctggagcgat
     7321 cgtaatcgga ccgcgtcttg gaaaatacaa tgcggatggt tccgtaaatc caattccagc
     7381 tcatcatgta cccatggctc ttctcggtgc gttcattctc gcattcgggt ggtttggttt
     7441 taatccaggt tccactctag gtgtttcggg aggtggactc aatcgagttg ggattgttgc
     7501 ggtggtgact atgctcgcag gtgccggtgg cgctctgtct gctactgcac tcataatctg
     7561 gaaagcaggc agaccagatc caaccatggt tgcaaacgga atgctggcag gcctggttgc
     7621 gatcacagct ccgtccggat ttgtaagtgc gccgattggt tttctgattg gtgcgattgc
     7681 gggtgtgctc gtagtgttgt ctgttgattt ctttgatcac ttgaaaatag atgatccagt
     7741 gggtgcgata tcagtgcacg gagtttgcgg atcgttcgga gttctttccg ttgggatctt
     7801 tgcggatgga accgctaact ttggtggatc ctggaacgga gtggccggca atgtcaaagg
     7861 tgcgatcttc ggagactggg ggcagctctt cgcacaattg attggcgtgg ccaccgtgat
     7921 tgtctggtct tttggcgtat cgtttatatt ctttaagatc ctgcacaagg tcatgggcat
     7981 gcgcgtgtcc gaacaggtgg agcttgacgg tctggatctg gacgaaaccg gaatcatggg
     8041 atatccgagt ttcccgaccg tggagtgatt gcgcgcacag tgcgcgattt catcacggtt
     8101 ctatgcgtca aaatatttca accggctcca agtgggagcc gatcattggt tactctcgtt
     8161 gcgtgaaagt tggcaaccat gtgtatgttt ccggaactac agcgtccggc ccgaatggat
     8221 ttgttggtga aggagatccc tacgctcaga ccaaacagat catcgaaaac atttcaaaag
     8281 cgctcgagca agccggcgct tccttgaagg acgtagttcg cacacgcatc tacatgacaa
     8341 acattgacca atgggaaatc gttggaaagg cgcacggcga attctttgga tcgatcatgc
     8401 ctgccactgc aatggtagaa gtcaaaaggc tgatttcccc cgagatactc gtggaaatcg
     8461 aagccgatgc agtcatccag ggctaagaag tccagacgac ttctcatagt cggcggcctg
     8521 attgttgccg ccggcattgg atggggagcg tttcgttctc tgaatcctta cgtaggactt
     8581 aagatctata agaatccagc gcgatgctat gcgctttcta tcccgggcca cgtgcagatc
     8641 caggcggagg atccttccca tgtcacattt gccgcggcgg attggactgg cggg
//
LOCUS       DAORFF010000086         8647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3903_length_8647_cov_3.179586, whole genome shotgun sequence.
ACCESSION   DAORFF010000086 DAORFF010000000
VERSION     DAORFF010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8647
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3903_length_8647_cov_3.179586"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKK76_05225"
     CDS             <1..453
                     /locus_tag="PKK76_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67902.1"
                     /translation="NRDADRPFLNHLEGKDSDWADSFKRVPRAFLEIIAISAASAALA
                     NFVVLPAYYLQISFPRFFATLLGLALAGLAAFYIRAYLKVARMQNPDVSASTAAAFLG
                     FRILANSLFITAMVSLVCLVIAMVVGIAMFNLNGLETIGVLKKASRLQ"
     gene            complement(654..1823)
                     /locus_tag="PKK76_05230"
     CDS             complement(654..1823)
                     /locus_tag="PKK76_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67903.1"
                     /translation="MGDRSDSTIALPARMVLTREGLSLFAKNPESIKKVKSRDGMQRE
                     GLEADAFNAPTVQKLIMNSYLEEIYVRLPDPLRRRAQIISTNNLVVYAVLYKKLSPSL
                     ARMLFESTVVREFNRKNPKNSIVDLKHIPSEKVDGLRQLKPGLFEQMEEEIHADVVDR
                     ISSNPALSEEDKSVRVRSLPKFIKWIDKRIWYLYFIIYQTAMKEQMRKAFGGMIAIYL
                     DHTKIATHLSNMLMEFVQNAEKSSLERIMVKNNLARREDVDRMLRDLDTRVKIVEIAS
                     KTDQMVDIAWNMNPERNSFGKQYRIQIIVSNYGLIDEVTRSNLARKMKTNVEGINIAS
                     FYQDSGDETKLGAGLGLLYNSYLEDICRAEGIQYKCNIFPEPEKEKTTVRVDLTM"
     gene            1929..3227
                     /locus_tag="PKK76_05235"
     CDS             1929..3227
                     /locus_tag="PKK76_05235"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNL67904.1"
                     /translation="MRITVVGTGYVGLVAGTCFSEYGNKVHCVDIDEAKISKLKQGIL
                     PIYEPGLSEMVVSNYAKSRLHFTTDLKSAVEDSDIIFIAVGTPDSGDGKPDLSGVLTV
                     ASKVGEYLNDYKVIVTKSTVPVGTADQVRAAATRTAKHPFDVVSNPEFLREGRAIDDF
                     MEPERIVVGCDSERSAAMMKELYNPFVQETGNPLIVMSIRSAELTKYACNAFLAMKIS
                     FVNEVANLCDALGANYLDVRTGMGTDSRIGKKFLNAGIGYGGSCFPKDVRALSSFAEA
                     CGLKIPLFDEVEGTNEKQKARLVDKILKHYGNVSGKVFAVWGLAFKAGTDDMREAPSL
                     TILNRLADLGATIRASDPVAVEVARPHLRDSITYGEMYSVIEGADALLLLTEWPAYLE
                     PDFKRMKSLLKEPVIFDGRNVYPPQSLKKMGFQYQGMGVA"
     gene            3224..3919
                     /locus_tag="PKK76_05240"
     CDS             3224..3919
                     /locus_tag="PKK76_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HNL67905.1"
                     /translation="MKHLGHRVFGKSVANRDIEVFRYGTGQLSILLMGGVHGDETEGT
                     FFVNKFRTELEKGKIDLPEGITLHICPQANPDGCADNRRTNHRNVDLNRNLPTKDWTG
                     AFTNVRFYPGPHAGSEPENQAILGMIAECMPSVIFSLHSYENPMVNFNGDSEDLGQAM
                     ARANGLPAKGDIGYPTPGSLGTYAGWERNIPTITLEILRGQAHEQVWSAHLEGMNVGL
                     SYYLTHKLPPRKG"
     gene            3922..4740
                     /locus_tag="PKK76_05245"
     CDS             3922..4740
                     /locus_tag="PKK76_05245"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNL67906.1"
                     /translation="MQNEEIKKLIEEAYDDRTRLPAAREAVLEALSLLNQGKIRVAVC
                     KAPGDWEVQAWVKKAILLYFGITEMKEVHSGDLSYFDKIPTRTDHKEAGIRAVPPAVI
                     RYGAFAEKGVILMPSYVNIGAYVATGTMVDTWATVGSCAQIGKSVHLSGGVGIGGVLE
                     PPQGRPVIIEDGAFLGSRAIVVEGVMIEERAVIGANVTLTASTPIIDVTGPSEVVYKG
                     RVPRDSVVIPGMREKKFAAGTFGVPCALIIGKRKATTDEKTSLNDALRDFEVSV"
     gene            complement(4758..5831)
                     /locus_tag="PKK76_05250"
     CDS             complement(4758..5831)
                     /locus_tag="PKK76_05250"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNL67907.1"
                     /translation="MKKVSELWGKFLRIPLFKVKFGVVRMDNMPFILFHLSPIFIFFV
                     PFKWELVGLAVGLYFVRMFFITGIYHRYFSHRGYEVRNRFIQFVMGLLGTTCVQQGPL
                     WWAEHHRHHHRYSETDNDIHSPVQYGFWHSHMWWFVTMYDNPKYHRYELKDFAKYPEL
                     KWLDNYHVIGPVLLGVALFGIGGAPYLVWGLSVSTVLLWHGTWTINSLSHVFGKKRYE
                     TGDESRNNPFLAILTLGEGWHNNHHAYQGGAKAGFYWYEYDITYYLLWCFHKLGIVTK
                     MGQPPERVLALGRANDAARRAARQLVGRRVLGKLTVEEVQLLVHAAEKGASAFRKKKV
                     GEIKAILASLREKAPMSTMPAMA"
     gene            complement(5828..6223)
                     /locus_tag="PKK76_05255"
     CDS             complement(5828..6223)
                     /locus_tag="PKK76_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67908.1"
                     /translation="MKDNESAWASLIETAIDSKPEPEVPAFLYPRMAKQLFPKPLSRA
                     MQWFVAILLIVSPPLFGYLSDSVYPGGTPDPGIVYTLYGILSLLWVLPVAGGIFHRSG
                     TSLVALSIQIDEIIKHPIRGIRTWSGVSK"
     gene            complement(6220..6711)
                     /locus_tag="PKK76_05260"
     CDS             complement(6220..6711)
                     /locus_tag="PKK76_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNL67909.1"
                     /translation="MESEREFERIFEETSRMIYSLGMRLFRNEEDAQDFIQDVYLEAY
                     RKRERYQGRSRYSTYLYSLALHYGLNRIRKRKLDFELLDEATTAAPEEEAPPEGDPSV
                     VREELGLLPEVYRLPIVLHYYEKMSIARIAESLDQNENTVKSYLHRGRKILKQKIEQR
                     KQS"
     gene            complement(6745..7140)
                     /locus_tag="PKK76_05265"
     CDS             complement(6745..7140)
                     /locus_tag="PKK76_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67910.1"
                     /translation="MNPVLEAQLIEAIKNLNAQLAHPPSDPARVILSLIPAFGIVFGS
                     VLLFFFFYWHFSIRRELIRAGLYRPSTFRNFRVFCLLVGSLATFVGIPMTVLFYALQG
                     LTYAMLGGLIPLGAGIGFLVFYALASREN"
     gene            complement(7185..8207)
                     /locus_tag="PKK76_05270"
     CDS             complement(7185..8207)
                     /locus_tag="PKK76_05270"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNL67911.1"
                     /translation="MRKSKPDLNPLYQWYAREHRKLPFRETRDPYRIWVSEIMLQQTR
                     VAAMLERYNQFIRRFPNIESLAKSSEEEVVLMWQGLGYYSRAKNLRKGAQLVTERWRG
                     KFPDTKEEALLIPGIGDYTASAVLSISHNSAYAAFDGNVRRVTARLFYPEAQNDRAAR
                     KKAQEVLSGAEPGMHNQAMMELGAVICVPGLPACDRCPISADCNAFSQGGRELAARIP
                     AKQKKEKLEVQMDFYSIEQGERVILVQDPEARFLPGHWMFPSRLRMTGIDRVTPGFSG
                     VVLREALGTIEHTITKHSIAGHLWRAGPPFDLSRVSWREVRKSELRDVLASSLGLKLA
                     RLIPET"
     gene            complement(8194..>8647)
                     /locus_tag="PKK76_05275"
     CDS             complement(8194..>8647)
                     /locus_tag="PKK76_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HNL67912.1"
                     /translation="GSFFFISVLLIGQELKPGVPALDRCGSCRICIDACPTGALTEYK
                     IDARKCISYLTIERRSPMPDELRPHVQGWMFGCDVCQEVCPYNRTASYGAGWPSPDPG
                     VVRLMESASEESLEDARANTPLGRPPLDLLKQSLQIAKESLDYLSEKI"
BASE COUNT         2201 a         2263 c         2145 g         2038 t
ORIGIN      
        1 aacagagacg cggatagacc tttcctgaat catctagaag gcaaggattc agactgggcg
       61 gattctttca aacgtgttcc ccgagcattt ctggaaatca tcgccatcag tgccgcatcc
      121 gccgcacttg caaacttcgt cgttcttccc gcctactatc tgcagattag ttttcctcgt
      181 ttctttgcca ctcttctcgg gctcgctctg gcgggccttg ctgcctttta catcagagcg
      241 tatctcaaag tggcccgcat gcagaatccg gatgtgagcg caagcacagc tgctgcgttt
      301 cttggctttc gcatccttgc aaactcgctg ttcatcactg ccatggtttc tctcgtctgc
      361 cttgtcattg ccatggtcgt gggaatcgcc atgttcaatc tgaatggtct ggaaaccatt
      421 ggcgttttga aaaaagcatc cagactgcaa tagtcgcctc gtgcggcccg gcggatgaac
      481 gcaatatccc gcctgtgcgg cccatcctcg cgtgcgcccc agcggcgctc ggacgctccg
      541 ttctccgctg tggaccgcac atgcgggata tcactacctc gcgggccttc gagactcgca
      601 atttcaaaaa acgacgcgca tctcatcaac gattcgcgca gcgaatcgat ccattacata
      661 gtcagatcaa ctctgactgt ggtcttttct ttctccggct cggggaagat gttacacttg
      721 tactgaatgc cttccgctct gcatatgtct tccagatagg agttgtacaa aagaccaagg
      781 cccgcaccca gctttgtttc gtctccgctg tcctggtaga agctggcaat attgattcct
      841 tccacgtttg tcttcatctt cctggcaaga ttggaccggg tgacctcatc aatgagcccg
      901 tagttagaca caatgatctg aatgcgatac tgcttcccga aagagtttct ttctggattc
      961 atattccagg cgatgtctac catctggtcc gtctttgacg caatttcaac aatcttcacg
     1021 cgcgtatcta gatcgcgaag cattctgtcc acgtcttctc gacgcgccag attgttcttc
     1081 accataattc tttccaggct ggatttctcc gcgttctgaa cgaactccat gagcatattc
     1141 gacaggtgag tggcgatttt cgtgtgatcc aggtagatcg cgatcattcc accaaacgcc
     1201 tttctcatct gctctttcat cgcagtctga tagatgataa aatagagata ccagattcgc
     1261 ttatcaatcc acttgatgaa ttttggcaga cttcgaactc tcacactctt gtcttcttct
     1321 gaaagagcag gattgctact gatgcgatcc acgacatccg cgtgaatttc ttcttccatt
     1381 tgctcaaaga ggcctggctt gagttgtctg agtccgtcta ctttctctga cgggatgtgt
     1441 tttaaatcta caatgctatt ctttggattc tttcgattga attcgcgcac caccgtggat
     1501 tcaaaaagca tccgtgcgag agacggactg agcttcttat agagaactgc atacacgacc
     1561 aggttattgg tactgatgat ttgtgcgcga cggcgaagag gatcaggcag gcgaacgtaa
     1621 atctcctcaa ggtaggagtt catgatgagc ttttgaacgg tgggggcatt gaaagcatcc
     1681 gcctcaaggc cctctctctg cattccgtcg cggcttttga ctttcttgat ggactcgggg
     1741 ttcttcgcaa acagagacag accttctctg gttaaaacca tgcgcgcagg aagcgcgatc
     1801 gtcgaatccg acctgtcccc catccccaca gcatgattca ggccatgcga atggggtcaa
     1861 ttgaattgca cacacgtgta agcctaaaaa agaatcgcct cggagacccg cgtcgacagc
     1921 ttcggaccat gcgcattacc gtggttggaa cgggctacgt cggactcgtg gcaggaactt
     1981 gtttctctga atatggaaac aaagtgcact gtgtggacat agacgaagcc aaaatcagca
     2041 agctcaagca gggaattctt ccaatttatg aaccgggtct ctccgaaatg gtcgtcagca
     2101 actatgcgaa aagccgcctt catttcacga ctgatctgaa aagcgcagtc gaggattcag
     2161 atataatatt catcgccgtg ggcacgcctg atagcggaga cgggaagcca gatctatccg
     2221 gagtactcac agttgcctcc aaagtgggag aatacctcaa cgattataaa gtcattgtaa
     2281 ccaaaagcac ggtccctgtg ggcacagcag accaggttcg cgccgccgcc acccgtactg
     2341 caaagcaccc ttttgacgtg gtgagcaatc cggaattctt gcgcgaaggt cgcgccattg
     2401 acgacttcat ggaaccggag agaattgtag tgggttgtga cagcgaacgg tccgctgcga
     2461 tgatgaagga actttacaat ccgtttgttc aggaaacagg aaatccactg attgtaatga
     2521 gcattcgctc cgcggagcta acaaagtatg catgcaacgc gtttctcgcg atgaagatct
     2581 cttttgtaaa cgaagtagcc aacctgtgcg atgcactggg agcaaactac ctggacgtgc
     2641 gcacaggaat gggaacggat tccagaatcg gaaagaagtt cctcaatgca gggattggct
     2701 atggtggctc ctgcttcccc aaagatgttc gcgcattgtc cagtttcgcg gaagcatgcg
     2761 gactgaagat accgttattc gacgaggtgg aaggcacaaa tgaaaaacaa aaagcgcgcc
     2821 tcgtagacaa gattctcaaa cactacggca acgtatccgg caaagtattc gcagtgtggg
     2881 gccttgcttt caaagcaggc acagacgata tgcgcgaagc tccttccctc accattctca
     2941 atcgtctcgc tgatctcgga gccaccattc gcgcaagcga tccggttgct gtggaagtgg
     3001 cgcgccctca ccttcgcgac agcatcactt atggagaaat gtattcagtg attgaaggag
     3061 cagacgctct ccttctcctt acagaatggc ccgcgtatct ggagcctgat ttcaaacgca
     3121 tgaagagtct attgaaagag cccgtgatct ttgacggaag aaacgtgtat ccacctcaat
     3181 ccctcaagaa gatgggattc cagtaccagg gtatgggagt agcatgaagc acctgggaca
     3241 cagggtgttt ggaaagtcag tcgccaatcg agacattgaa gtgtttcgct atggaaccgg
     3301 acagttgtct attttactga tgggtggcgt tcacggagat gaaacagaag gcacgttctt
     3361 tgtgaacaag tttcgcactg aactggagaa aggaaagatc gacctgcccg aaggaatcac
     3421 tctgcacatt tgcccgcagg caaatccgga cgggtgcgcg gacaacagaa gaaccaatca
     3481 cagaaatgtg gatctcaatc gcaacttacc aacaaaagat tggacgggcg catttacgaa
     3541 cgtgcgattc tatcctggac ctcatgctgg aagcgaacct gaaaaccagg caatccttgg
     3601 tatgatcgca gaatgcatgc cttctgtcat tttctctctt cactcatacg aaaacccgat
     3661 ggtgaatttc aacggagact cggaagacct gggccaggcc atggcaaggg ccaatggact
     3721 ccctgccaaa ggagacattg gatacccgac tccaggaagc cttggaacgt acgcaggctg
     3781 ggagagaaac attcctacga tcacactgga aattctaaga ggccaggcgc acgagcaagt
     3841 gtggtctgct catctggaag gaatgaatgt gggtctgtct tactacctca ctcacaaatt
     3901 gcctccgcgc aaaggttgat catgcaaaac gaagaaatca agaaactcat agaagaagca
     3961 tacgatgaca gaaccaggct gcccgcagca cgcgaagcag tactcgaagc tctttccctt
     4021 ctcaatcaag ggaagattcg cgtagcggtc tgtaaggcgc ccggggactg ggaagtccag
     4081 gcctgggtga aaaaagcaat ccttctctat tttggaatca cggaaatgaa ggaagtgcat
     4141 tccggcgatc tttcctactt tgacaagatc ccaacacgca cggatcacaa agaggcgggc
     4201 attcgcgcag ttccacccgc tgtgattcgc tacggcgcat ttgcagaaaa aggcgtcata
     4261 ctcatgccat cttacgtgaa cattggagct tatgtggcaa caggaaccat ggtagacacc
     4321 tgggcgaccg ttgggtcctg cgcgcaaatt ggaaaatctg tgcacctgag cggcggagtc
     4381 ggaatcggtg gagtgcttga accaccccag ggaagaccgg tgatcatcga agacggagct
     4441 ttccttggat cgcgcgcgat tgtagtggaa ggtgtgatga tcgaagaacg cgccgtgatc
     4501 ggggcgaatg tgactctcac tgcatctact cccatcattg atgtaacagg accttcggaa
     4561 gtagtgtaca aaggacgcgt ccccagggat tcagtggtca ttcccggaat gcgcgaaaag
     4621 aaatttgcgg ctggaacatt tggagtgccc tgcgcactca tcattggaaa gcgcaaggca
     4681 actacagatg agaagacatc cctgaacgat gcgctcaggg attttgaagt gagcgtctaa
     4741 cgtctgcggc caggccctca ggccatggcc ggcattgtgg acatcggagc tttttcccgg
     4801 agagaggcaa ggatcgcttt gatttctcct actttcttct tcctgaaagc agatgctccc
     4861 ttctctgctg cgtgcacgag cagctgaact tcttccaccg tcaatttccc gagcacccgc
     4921 ctgcccacca gctgtcgcgc agcgcgacga gctgcatcat ttgcacggcc cagtgctaga
     4981 actctttcag gtggttgtcc cattttcgtt acaatcccaa gcttgtgaaa acaccagaga
     5041 agatagtagg taatgtcgta ctcgtaccag taaaaacctg cttttgcacc accctgatat
     5101 gcatggtgat tgttatgcca tccttctccc agagtcaaaa tggccagaaa cggattgttg
     5161 cgcgattcgt ctcctgtctc gtatctcttt tttccaaaca catgggagag agagttgatg
     5221 gtccaggttc catgccatag cagcacggta gaaacagaaa ggccccagac gagataaggc
     5281 gcgccgccaa tgccaaagag ggccacaccc agtagaaccg ggccgatcac atgatagtta
     5341 tccagccatt tcagttcagg atatttggcg aagtctttca attcgtagcg atgatacttt
     5401 ggattgtcat acatggtaac gaaccaccac atatgcgaat gccagaaacc atactgtacg
     5461 ggggaatgaa tgtcattgtc cgtctccgag tagcgatggt ggtgtctgtg gtgttccgcc
     5521 caccacagag gcccttgctg cacgcaagtt gtgcccagta gtcccatcac gaattgaatg
     5581 aaacgatttc gcacctcata cccgcgatga ctgaaatacc tgtgatagat gcctgtaata
     5641 aagaacatcc gcacgaaata caggccgact gccagaccaa ccagttccca tttaaacgga
     5701 acaaagaaga taaaaatagg agaaagatgg aacaggatga atggcatatt gtccattctc
     5761 actactccaa acttcacctt gaataaggga atgcgaagaa actttcccca gagttcactg
     5821 accttcttca ttttgatact cccgaccatg ttctgattcc tcgaattgga tgctttatga
     5881 tttcatcgat ctgaatggac aaagcgacaa ggctcgtgcc acttctgtga aagattcctc
     5941 ccgcgacggg aaggacccat agcagagaca gaatgccgta taacgtgtag acaattccag
     6001 gatccggcgt gccgccagga taaacagaat cagacaggta tccgaataga ggcggactga
     6061 caatcagcag aatcgctaca aaccattgca tcgcacgcga aagtggtttt ggaaacaatt
     6121 gttttgccat tcgcggatac aggaatgccg gcacctcagg ttcaggtttc gaatcaatgg
     6181 ctgtctctat gaggctggcc cacgcggatt cattatcttt catgattgtt tcctctgctc
     6241 tatcttctgc ttgagaatct ttcttccgcg atgtaaatac gacttcacag tattctcatt
     6301 ctgatccagg ctttctgcaa tcctggcaat ggacatcttt tcatagtaat gcaaaacaat
     6361 cggaagtctg tagacctctg gcaacagtcc cagctcttcg cgcacaacgg atggatctcc
     6421 ttcaggcggc gcctcttcct ctggagccgc agtggtggct tcatccagca attcaaagtc
     6481 gagctttctc ttgcgaatgc gattgaggcc atagtgaagc gcgagagaat agagatatgt
     6541 cgaataacgg gaacgtccct ggtatctttc tcgttttctg tacgcttcca ggtagacgtc
     6601 ctggataaaa tcctgagcgt cttcctcgtt gcgaaataat ctcattccaa gcgaatagat
     6661 catgcgggat gtctcctcga agatccgctc aaattcccgt tccgattcca agagagccca
     6721 gtagaccgat gaggccgcgc cctgtcaatt ttcacgagat gcaagcgcat agaatacgag
     6781 aaatccgatc ccggccccca ggggaatgag accgcccagc atggcatacg tgagaccttg
     6841 cagggcgtag aaaagtacgg tcattggaat tccaacgaat gtggccaggc ttccgaccaa
     6901 aagacagaag accctaaagt tgcgaaaggt ggaaggccga tacaatccag cgcggatcag
     6961 ctcccggcga atggaaaagt gccagtagaa aaagaaaaac aatagaacag agccaaatac
     7021 gattccaaac gcaggaatca agctcaagat caccctggca ggatcagagg gcgggtgcgc
     7081 cagttgcgcg ttgaggttct tgatcgcttc gatcagttgt gcttccagta ccgggttcat
     7141 tcaaagcatg agaaccgcag gcggaagagc ggttgcaaaa aatttcaggt ttctgggatc
     7201 aatcgagcca gtttcagccc gagagaagaa gccagtacat ccctcagctc tgactttcgc
     7261 acctcgcgcc aggaaaccct ggacaaatca aaaggaggac cggcccgcca gagatgccct
     7321 gcaatggaat gctttgtgat tgtgtgttcg atcgttccca atgcctcgcg aagtaccaca
     7381 cctgaaaagc ctggagtgac gcgatctatc ccggtcatgc gaagcctgga cggaaacatc
     7441 cagtggcccg gcaaaaacct ggcctctgga tcctgaacca gaatcacccg ctcaccctgc
     7501 tctatggaat aaaaatccat ctgaacctcg agcttctctt ttttctgctt cgcaggaatc
     7561 cgggcggcaa gctcacgtcc accttgcgaa aacgcattgc aatcagcgga aatggggcag
     7621 cgatcacagg caggcaaacc cggaacacaa atcacagcac ccagctccat catcgcctgg
     7681 ttgtgcattc ctggctctgc accggataag acttcctgag ccttttttcg cgcggctctg
     7741 tcgttttgcg cttcaggata aaagagacga gctgtcacgc ggcgaacatt cccgtcaaat
     7801 gccgcatacg cactgttatg cgaaatactg agaactgcag aagcagtata gtcaccaatg
     7861 cccgggatca ggagggcttc ctctttagta tccggaaact tgcctctcca tctttcggtc
     7921 accagttgcg cacctttcct gagattcttc gcgcgactgt agtaacccag gccctgccac
     7981 atgagcacga cttcttcctc agagctcttc gcgagcgatt ctatgttcgg aaatcgtctt
     8041 atgaattgat tgtaccgttc tagcatcgca gccacacgag tctgctgcag catgatctca
     8101 gaaacccaga ttctgtaggg atcacgcgtc tctctgaagg gaagtttgcg atgctccctg
     8161 gcataccatt gatacagcgg attcagatca ggcttagatt ttctcactga gataatccag
     8221 agattctttt gcgatctgca aactctgctt gagtaagtcg agaggaggcc gacccagagg
     8281 ggtattggct cgcgcatctt cgagagattc ctctgaagcc gactccatca aacgaaccac
     8341 cccgggatct ggcgagggcc acccggcacc gtaagacgca gtgcgattgt agggacagac
     8401 ttcctggcac acgtcgcagc cgaacatcca accctgcacg tgcggacgca gttcatcggg
     8461 catggggctt cttctttcaa tggtcagata cgatatgcat tttcgcgcgt cgattttgta
     8521 ttcagtcagt gcgccggttg gacatgcatc tatgcaaatt ctgcaagaac cgcatcgatc
     8581 cagggcagga acacccggtt tcaattcctg tccgatgagc aaaacgctga tgaagaaaaa
     8641 agatccc
//
LOCUS       DAORFF010000087         8628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3921_length_8628_cov_3.708853, whole genome shotgun sequence.
ACCESSION   DAORFF010000087 DAORFF010000000
VERSION     DAORFF010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8628
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3921_length_8628_cov_3.708853"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1680
                     /locus_tag="PKK76_05280"
     CDS             142..1680
                     /locus_tag="PKK76_05280"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNL67913.1"
                     /translation="MTSRIVFVLLSIFALPLAAQSELDSTMKSLRTDVRRVTLENGLR
                     IIMVRRPFSPTVACYMKFKAGGADETEESAGIAHMLEHMLFKGTERVGTLDFEKEKKY
                     IEQSNVWADRLDDARRALDAAEKSGDTTRIAEAKAEVQKRKQRLMSLQQMARKYMIPD
                     EDSYIYAQAGERGYNAYTTKDLTNYQVELPANRLEIWAKLESDRMENSVLRDFYTERE
                     VVREERRLRVDNVAQGALLEQFIKDIYGSHPYGRSLIGSMQVIQYLNFGQAKSFYNTY
                     YAPNNTVIALVGDIDLAQTEALMRKYFSHLKRKDIPERPSVAVAPSRARTEIEKPGSP
                     VMFLAWNKPSGMTKDSLSLEVLSSILAGRSDSRLVRKLVMKDRSAAEVSAFTGYPGER
                     HPNLLTIQAVPAPGKSYDDVQKGVLEEIASVQKDGVTQDELDRVKASMVADFLYGLRS
                     NAEYADNLSYYELLYGNYETLFTKITALSAIAPADVKKAAIEYMTPDKMHSARLKQAK
                     GEEQ"
     gene            1677..3122
                     /locus_tag="PKK76_05285"
     CDS             1677..3122
                     /locus_tag="PKK76_05285"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNL67914.1"
                     /translation="MKRSLILFLLPAALMAQQTRPPANLSSKESREHAEKLIADIPST
                     PIRFAPPPVRQTTLPSGATMLVTEEKSLPFLTVELHFKGGNSLEKVDHAGLWDAATSL
                     LAVDGAGSRSGDEIAEEMSRLGARFSIRGDHESWVVSITALKWNFPKAFAIVSDILLK
                     PKLSEERLAVILDGMETDIDQRNNRPESIAGRRLLEVMYPNSRRGYVLQKTDLKKITP
                     QLIREDLNRRLYAPGVIVACAGDVSSVPVESMVNGLLAQFPAKSAPEQIQKPAGAPLL
                     AGKILLVTAEVPQASVAMGTILPGHSSEDFYALQVANHILGGNSFNSRLMKEVRTARG
                     LAYYSMSRTTFFKDDGRLTAASGTRSEQANETVKLMLEIIGGMRQPADAVELNLARES
                     ILNSLVFLYDDPESFLQSQVRFKLHGMPDDYLARYADHIRALTAEDVSRAYARHVHSD
                     MTIVVVGPKSLAPELQKIRPVVMVTPEGKLP"
     gene            3202..3957
                     /locus_tag="PKK76_05290"
     CDS             3202..3957
                     /locus_tag="PKK76_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL67915.1"
                     /translation="MILPTFHLAFDAGRCQNQLVEIPTVLLTHGHLDHASGIPYYVSQ
                     RCLRHLPGGTIYCPPELEDPLRRILRIWSEIEDYQMEFSLQGVDYSTFYPLFGNFSFR
                     AVRSVHRVASNGYTIYERVRKLRKEYSHLTGPQIAEMKKTMNLFEDVQSPVVTFSGDT
                     QIEFALENEDVRRSKILFLECTYIDEARPVERARKWGHTHLFEIIENAEAFKEVEHLV
                     LIHFSPRYSKDRILDTLRKQMPEDLYRKTTAFV"
     gene            3957..4313
                     /locus_tag="PKK76_05295"
     CDS             3957..4313
                     /locus_tag="PKK76_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67916.1"
                     /translation="MSSNPYSPPDTKAPLEEGERLLTPTGILRFWEIGRIPYNGILFA
                     EAVFVSQGIPKMAEIAFYGIIINALYCFGPFVDFYFLAIAHHPLPRILRYALYVLGTI
                     LCAGSMFVILAAASAS"
     gene            complement(4314..5336)
                     /locus_tag="PKK76_05300"
     CDS             complement(4314..5336)
                     /locus_tag="PKK76_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNL67917.1"
                     /translation="MKQILQLSLAQLRGFLREPGILFWAFAFPILMAWVLGAAFDKKG
                     EGPVSVSLVQTSPAALRPTAGASLKKDPLIHLREESADAALLALRRGESQIIVEENSA
                     GVKVRFDPANQEAVLARYRAERALSKNQLPVNVEQITARGSRYVDFLVPGLVALGIMN
                     SCMWGIGWLMIEFRMKKLLRAMIAASMKRIQYLISFFVTRIIANTVETGLLLLFAALY
                     FGIRIEGTLLAFMMVIVAGLVAFTGIAILGACRAASTQVGNGILNAVTLPLMILSGVF
                     FSYHNFPDAVVPIIRYLPLTLLADSLRAVFVEGAGVAQVWLSCAILCITGAITFVAGL
                     RFYRWY"
     gene            complement(5333..6214)
                     /locus_tag="PKK76_05305"
     CDS             complement(5333..6214)
                     /locus_tag="PKK76_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004508712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNL67918.1"
                     /translation="MKTFRALRAVDGVDLTIRRGEYVGLLGPNGAGKTTLVEMIEGIQ
                     APDDGEIHIFGKSWADSAEELRKRVGLCLQETHFMDKIRVRETMKLFGSFHDSTEERV
                     DEMLGLVNLTDKSRDFVVNLSGGQKQRLALGIALLSRPEILILDEPTTGLDPHARREI
                     WKILGDIRNAGTTLILTTHYMEEAEHLCERIVVMDKGRVLADGPKRELLASMGGETIS
                     FRAKKKIAEKSLRRVPGFLSYIPQDGGGRVFVSRMRDALPVFLSAAKSAGITDIECRR
                     STLDDVFIHMTGRTLNE"
     gene            complement(6296..7483)
                     /locus_tag="PKK76_05310"
     CDS             complement(6296..7483)
                     /locus_tag="PKK76_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67919.1"
                     /translation="MFRHNVFLLIFAVSVLQCKESKAEVNPDMRKVVCLNSEQGCPCK
                     TDNGEFRIFHAEAARVTGPSVGGLLEIVVRGKSCRYPASALVVMPYAPVLTSPWQKAD
                     HSPMAVDRLTGISRTDLDQAERFSLGRFWPTFYHLALEEFYPGQDVPILSADGRVLGR
                     ASDQFLQQVTWEGSGISRTGLRLAYAGPGRYSTYKNDVWGYGAGGRTQILPYRTIAVN
                     FPGLCKRLSPKANCSAQTIPGIMLFVPELAKKKIRMQDGSFHDGYLCATDTGSPYYIR
                     EDRIDMFVGSHGGGNPFLPMERQLNDYIRGGLEAILPSDFRVWTGVNERVWCSPELIP
                     KDPFHPAAGECKIDYHVVAAHKSLEMFALIGKDGKPLRCLGSPPSPGPHFPDGKEPAQ
                     AQK"
     gene            7598..8140
                     /locus_tag="PKK76_05315"
     CDS             7598..8140
                     /locus_tag="PKK76_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67920.1"
                     /translation="MAKKAKKSGSGSAKSEAEKKKLLSAKNPDQYIQRSLDSKLSRGE
                     KGSLTREWLEKTGYTHEDILYARNRNPYWKKQKGRGSYERTLKRIKKLNFGGKSTGRK
                     VWSDADLGRFYELNDKMADWQLAQKFKTSLPAVNHIRRKFKLAKALLEKQRKTVNKGA
                     VVKLSRTDEKVLRTTLAGKR"
     gene            complement(8159..>8628)
                     /locus_tag="PKK76_05320"
     CDS             complement(8159..>8628)
                     /locus_tag="PKK76_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNL67921.1"
                     /translation="DIEEVLSLGISRVILGTRAARSPGDIAGWISNFGADKIVIGVDV
                     AGDRIRVQGWEEDGGYSTSDFLALLESAGVRLIVATNISSDGTLEGVDLEFYKRITDA
                     SRLEFVVSGGVAGIQDVVNLKQWNHPQIHGMIVGKAYYEGRMDLVEAVRLAST"
BASE COUNT         2336 a         2313 c         2074 g         1905 t
ORIGIN      
        1 cgaactaccg accccttcct tatcagagaa gtgctctaac caactgagct actggcccgg
       61 acttgctgga ccagtttttc tacttgcgca ttggcggtca aacaactctt tacagagaac
      121 tctccctcaa aaggatcgcc tgtgacttcc cgcatcgtat ttgttttgct ctccatcttt
      181 gccctgcctc tggccgcgca atctgaactg gactccacca tgaaaagcct tcgcacggat
      241 gtgcgcagag tgactctgga aaacggtctt cgcatcatca tggttcgacg gcctttttcg
      301 cccacggtcg cctgctacat gaaattcaaa gcgggcggag cagatgaaac agaagaaagc
      361 gcgggaatcg cgcatatgct cgaacacatg ctgttcaaag gaacagagag agtgggtact
      421 ctggacttcg agaaagaaaa gaaatacatt gaacagagca acgtctgggc agacagactc
      481 gacgacgcca gacgcgccct ggatgcagca gaaaaaagtg gagatactac tcgcattgcg
      541 gaagcaaagg cggaagtcca aaagcgcaag caacgcttga tgagtctcca gcagatggcg
      601 cgcaaataca tgatcccgga tgaagattcc tacatctacg cgcaggccgg agaacgcggc
      661 tacaacgcgt acactacaaa ggacctcaca aactaccagg tggaattgcc tgccaatcgc
      721 cttgaaatat gggccaaact cgaatcagac agaatggaga actctgtgct tcgtgatttt
      781 tacacggaga gagaagttgt tcgcgaagaa agaagactgc gcgtggacaa cgtggctcag
      841 ggcgcgcttc tggaacaatt catcaaagac atttacggat cgcatccgta cgggcgctcg
      901 ctcatcggga gcatgcaggt cattcagtat ttgaattttg gtcaggcgaa atcattctac
      961 aacacctatt acgcccccaa caatactgtc atcgcactgg tgggagacat tgacctggcg
     1021 caaacagaag cgttgatgcg taaatacttc tctcatttga aaagaaaaga catcccggag
     1081 agaccttctg ttgccgtggc tccctccaga gcacgaacag aaatagaaaa gccaggatcc
     1141 cctgtgatgt ttctggcgtg gaacaaacca tccggcatga ctaaagacag cctgagtctc
     1201 gaagttctat cgagcatcct tgcaggtaga agcgacagca gacttgtgcg aaagcttgtg
     1261 atgaaagaca gaagcgcggc tgaagtttcc gcattcacag gatatccagg agaaagacat
     1321 cccaatctac tgaccattca ggcagtcccg gcaccgggca aatcctacga tgatgtccag
     1381 aagggagtac tcgaagaaat cgcttctgtt caaaaggacg gcgtcaccca ggatgaactc
     1441 gatcgggtca aagcatccat ggtcgcggac ttcctctacg ggctcagatc caacgcagaa
     1501 tatgcggaca acctttcgta ttacgagttg ttatacggaa attatgaaac actcttcact
     1561 aagattacag cactgagcgc cattgcgcct gcagacgtaa agaaagcagc catagaatat
     1621 atgacaccag acaaaatgca ttccgctcga ctcaaacagg caaagggaga agaacaatga
     1681 aaagatctct gatcctcttt ctcctgcccg ccgcactgat ggcgcaacag acaagacctc
     1741 cagccaatct ttcttccaag gaatcgcgcg aacacgcaga gaagctcatc gcagacattc
     1801 catccactcc gattcgattt gcgccgcctc cggtacggca gaccactctt ccttccggtg
     1861 ccacgatgct tgtaacggaa gaaaagtccc tgcccttttt gactgtggaa cttcacttca
     1921 aaggaggcaa ctcactcgag aaagtggatc atgcaggact ctgggacgca gccacaagct
     1981 tgctcgctgt ggacggagcc ggttcaagat ccggagatga aatcgcagaa gaaatgagcc
     2041 gactgggcgc tcgttttagc attcgcggag atcatgaatc ctgggtggtg agcatcactg
     2101 cattgaaatg gaatttcccg aaggcattcg ccattgtttc ggacattcta ctgaagccaa
     2161 agctgtcgga agaacgcctg gccgtgatcc tggacggaat ggaaacagac attgaccaga
     2221 gaaacaacag acctgaatcc atcgccgggc gaaggctgct cgaagtgatg tatccgaaca
     2281 gcagacgcgg ttatgttctg caaaaaacag atctaaaaaa aatcacgcca caactcatac
     2341 gcgaagatct caatcgcaga ttgtatgctc ctggtgtgat cgttgcgtgc gcaggggatg
     2401 tgagttctgt tccagtcgaa tccatggtca atggtcttct ggcacagttt ccggcaaagt
     2461 ctgcgccaga acaaattcag aaaccggccg gcgctccact tcttgccggc aaaatcctgc
     2521 ttgtgaccgc agaagttcct caggcatccg ttgccatggg gactatcttg cccggacact
     2581 cgtctgagga tttttatgcg ctgcaagtgg ccaatcatat cctgggtggc aattcgttta
     2641 acagccgttt gatgaaagag gtgcgcaccg cacgcggact ggcatactac tccatgtccc
     2701 gcactacgtt cttcaaggat gatggcaggc tgactgctgc atccggcact cgctctgagc
     2761 aggcgaatga gactgtaaag ctcatgctcg aaatcattgg cggcatgaga caaccggcgg
     2821 atgccgtgga actgaatctg gcgcgagaaa gcattctgaa ttcacttgta tttctatatg
     2881 atgatcctga aagtttttta caaagccagg ttcgcttcaa gttgcacggg atgcctgacg
     2941 attacctcgc tcgctacgct gaccacattc gcgctctcac tgcagaagac gttagccgcg
     3001 catacgcgcg acacgttcac tctgacatga cgattgtggt tgttggcccg aaaagcctcg
     3061 cacctgagct gcaaaaaatt cgccccgttg tgatggttac accggaaggc aagcttccct
     3121 gagcacgcat tgagcctcag agaatacaca atacgcaatc agcgtttcct gggaatctcc
     3181 agcggtggag tggaaacgag tatgattctg cccaccttcc atcttgcatt tgatgcaggg
     3241 agatgccaga atcaactcgt cgaaattcca accgtgctgc taactcatgg ccacctggat
     3301 catgcatccg gtattccgta ctacgtatcg cagagatgcc tccgtcatct tcctggagga
     3361 accatctact gccctccaga gctagaggat ccacttcgca gaatcttgcg catctggtcc
     3421 gaaatcgaag actaccagat ggaattcagc ctgcagggcg tggattactc cacgttttat
     3481 cctctcttcg gcaacttttc gtttcgtgcg gtgagaagcg ttcacagagt tgcatccaat
     3541 ggatatacca tctacgagag agtgcgaaaa ctaaggaaag aatacagtca cctcaccggc
     3601 ccccagattg cggaaatgaa aaaaacaatg aatctttttg aggacgttca atcgccagtg
     3661 gttactttct ccggagatac acagatagaa ttcgctcttg aaaatgaaga cgttcgaaga
     3721 tccaaaattc tatttttgga atgcacctat atagacgaag cgcggcccgt ggaaagagcg
     3781 agaaagtggg gccacactca tctttttgaa atcatcgaaa atgcagaagc cttcaaggaa
     3841 gtagagcatc tggtcttgat tcatttctct cccagatact cgaaggacag aatccttgac
     3901 actctcagaa aacaaatgcc agaagacctt tatcgaaaaa ccactgcatt cgtataatga
     3961 gttccaatcc ttattctcca ccggatacaa aggcacccct ggaagagggg gagcgtcttt
     4021 tgacgcccac gggcatcttg cgcttctggg aaattggaag aattccgtac aacggcattc
     4081 tattcgcaga ggcagtcttt gtctcccagg gaattccaaa gatggcagag attgcgtttt
     4141 acgggatcat cataaacgca ctgtattgct ttggcccttt tgtggacttt tactttctgg
     4201 cgattgcgca tcatccactc ccgcgaatcc tgcgatatgc actctatgtt ttgggaacca
     4261 tcctgtgcgc cggttccatg tttgtaatac tggcggcggc ttccgcgagt tgatcaatac
     4321 cagcgataga atcgtagacc ggccacaaat gtgatcgcac cggtaatgca aagaatcgcg
     4381 caggacaacc atacctgagc cactcctgct ccttccacaa acacggcacg gagtgaatct
     4441 gcaagaagag tcaatggcaa ataacgtatg atcggaacca ctgcgtccgg aaaattatga
     4501 tagctgaaga acacaccgga aagaatcatc agaggaagag tcactgcgtt tagaattcca
     4561 tttcccacct gggtgcttgc ggcacgacag gctccgagaa tcgcgatgcc ggtaaaggcg
     4621 actagcccgg ccacaatcac catcataaaa gcaagcagcg ttccctctat tctgattccg
     4681 aaatacaatg cagcaaacag cagaaggagg cctgtttcca cagtgtttgc tatgattctg
     4741 gtaacgaaaa aggaaattaa atactgaatc cgtttcatgg aagcagcaat catggcgcga
     4801 agaagcttct tcatgcgaaa ctcaatcatc agccagccaa taccccacat gcaagaattc
     4861 atgattccaa gagcgaccag accagggacc aggaaatcca catagcggct ccctcttgcg
     4921 gtgatctgtt ctacattcac aggcaactgg tttttggata gagcgcgttc tgccctgtat
     4981 ctcgcgagaa ccgcttcctg gtttgcagga tcaaaccgca ctttcactcc agctgaattc
     5041 tcttccacga tgatctgaga ttcaccgcgg cgaagagcaa gaagagctgc atccgcactt
     5101 tcttcgcgca gatgaatcaa tggatctttt ttcagagagg caccggcagt cggacgaagg
     5161 gctgctggac tcgtttgcac gagagacaca gacacagggc cttccccttt tttgtcgaat
     5221 gctgcaccaa gcacccaggc catcagaatc ggaaatgcga aagcccagaa taagatgccc
     5281 ggctctctca ggaaaccgcg caactgagcg agagagagct gcaaaatctg tttcattcgt
     5341 tcaaagtcct tcctgtcata tggatgaaaa catcgtccag ggtacttctt ctgcattcga
     5401 tgtcagtgat tcccgcagac tttgccgcgg ataaaaaaac cgggagagcg tcacgcattc
     5461 tgcttacgaa aactctgccg ccaccgtcct gtggaatgta ggaaagaaat ccgggcacgc
     5521 gccgcagtga tttttccgcg atttttttct ttgcgcgaaa cgaaatggtt tcaccgccca
     5581 tcgaggccag gagttcacgc tttggcccgt ccgcaagaac acgacctttg tccatcacaa
     5641 cgattctttc gcacagatgc tccgcttctt ccatgtagtg agtggtgaga atcaaagtag
     5701 ttcctgcgtt gcgaatatct cctagaatct tccatatttc acgacgagca tgcggatcca
     5761 gacctgtggt tggctcatct agaatcaaaa tctcagggcg cgaaagaagt gcaatgccca
     5821 gagccagcct ctgcttctgg cctccagaaa gattcactac gaaatccctg gacttatctg
     5881 tgagattcac gagacccagc atctcatcga ctctctcttc cgtggagtca tgaaagctac
     5941 caaacaactt catggtttcg cgtacgcgaa tcttgtccat gaaatgcgtt tcttgaaggc
     6001 agaggcccac tctctttcgc agctcctccg cggaatctgc ccagctcttg ccaaaaatat
     6061 gaatttctcc gtcatccgga gcctggatgc cttcgatcat ttccacgagc gtggttttgc
     6121 cggctccatt tgggccgagc aaaccgacat attctccgcg acgaatggtc aaatccacgc
     6181 cgtctactgc gcgaagcgct cggaaggtct tcacgactcc cttcagttca attacgatcg
     6241 ggtgcacgct gcaataaaat tcacgctggt cccagcgtca gctattttgc ctgaattact
     6301 tttgcgcctg agctggttct ttgccatccg ggaaatgtgg accaggagat ggaggcgatc
     6361 caaggcagcg aagtggcttg ccgtcttttc caatgagcgc aaacatctcc agagatttgt
     6421 gggcggccac gacatggtag tcgatcttgc actcgcccgc tgccggatga aacggatctt
     6481 taggaatcaa ttcaggagag caccagacgc gctcattgac gcccgtccac acgcgaaaat
     6541 cagacggaag aatggcctca aggcctccac gtatgtagtc gtttaactgg cgttccatgg
     6601 gaagaaacgg atttcccccg ccgtgagatc caacaaacat atcgattcta tcttcgcgaa
     6661 tatagtaagg cgaacctgta tccgttgcgc aaaggtatcc gtcatgaaaa gagccatcct
     6721 gcatgcgaat tttctttttc gctagctctg gaacaaaaag catgatcccg gggatcgtct
     6781 gtgcgctgca atttgcctta ggcgaaagcc ttttacacag accagggaaa ttcacagcga
     6841 ttgtgcgata cggaagaatc tgtgttctgc cgccggctcc gtagccccat acgtcattct
     6901 tgtaagtcga ataacgtcct ggccccgcat aggccagacg caaaccggta cgcgaaatgc
     6961 cgctgccctc ccaggtcacc tgctgcaaaa actgatccga tgcccgcccc agaacccgtc
     7021 cgtctgcaga aagaatcgga acgtcctgtc cagggtagaa ttcctccaat gctagatggt
     7081 agaaagtggg ccagaatcga ccaagagaaa atctctcggc ctggtccagg tccgtccgtg
     7141 aaataccggt aaggcgatcc actgccatgg gagagtgatc cgctttctgc cacggagaag
     7201 tcagtaccgg agcatagggc atgactacaa gcgcggaagc cggataacgg caggatttgc
     7261 cgcgtacaac tatttcgaga aggccaccga ccgaaggccc ggtaacacga gccgcctcag
     7321 catgaaaaat ccgaaattca ccgttatccg tcttacaggg gcatccctgc tcagaattca
     7381 ggcaaaccac ctttcgcatg tcaggattga cttctgcctt gctttctttg cattgcagga
     7441 cagagactgc gaagatcaac aggaacacat tatggcgaaa cacactgcat catcgcacag
     7501 cgcctgcccc agtgcaagca aaactggttg acgaagagtg cggttacaga actcctgtga
     7561 atatcgccgt taggcgacca tctcaaggcg gcatagtatg gcaaaaaagg caaagaaatc
     7621 aggatcaggc tctgcgaaat cagaagcaga aaaaaagaaa ttactttcgg caaaaaaccc
     7681 tgatcagtac atccagagat ctctggattc taaactcagc cgcggcgaaa agggcagtct
     7741 gacgcgcgaa tggctggaga aaacaggata cactcacgaa gacattctgt atgcacgcaa
     7801 tcgtaacccg tactggaaaa agcaaaaagg tcgtggcagc tacgagcgca cactgaagcg
     7861 aatcaaaaag ctgaacttcg gtggcaagag caccggacgc aaagtttgga gcgacgcaga
     7921 tctgggccgc ttctacgaac tcaatgacaa gatggcagac tggcaactgg cacagaaatt
     7981 caagaccagc cttcctgccg taaaccacat tcgccgcaag ttcaagctcg cgaaggctct
     8041 cctcgagaaa cagcgcaaga cagtgaacaa aggtgcagta gtgaaactca gtcgcacaga
     8101 tgagaaggtt cttcgcacca ctctcgcagg aaaacgttaa tagtttagat tctttagatt
     8161 aagtcgaggc cagtcgaacg gcctcgacaa gatccatccg gccttcatag taagctttac
     8221 ctacaatcat tccgtgaatc tgcggatgat tccactgctt caaattcact acatcctgta
     8281 tcccggccac gccacccgac accacgaact ccagccgcga agcatccgta atccttttat
     8341 agaactcgag atccacgccc tccagggttc cgtctgagga aatgttcgta gccacaatca
     8401 gcctcacacc cgccgactca agcaacgcca ggaaatcaga cgtcgaatat cctccgtctt
     8461 cttcccagcc ctgcacgcga atcctgtctc cagctacatc tacgccgatt acaattttat
     8521 ctgcgccaaa gtttgaaatc cagcctgcaa tgtcaccggg agacctggct gccctggttc
     8581 caagaatcac tcgcgaaatt ccaagagaca agacttcttc aatgtctg
//
LOCUS       DAORFF010000088         8586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3960_length_8586_cov_3.635564, whole genome shotgun sequence.
ACCESSION   DAORFF010000088 DAORFF010000000
VERSION     DAORFF010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3960_length_8586_cov_3.635564"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="PKK76_05325"
     CDS             <1..1157
                     /locus_tag="PKK76_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNL67922.1"
                     /translation="ASELRDVELVHIHTEGGAPYAKPEFAASFKTNVFFVGGNIREAV
                     QNHRADYVPVFLSEIPALFRKKILPIDVAMIHVSPPDNHGYCSLGVSVDIARAAVQCA
                     GKIIAQVNPAMPRTHGESMVHVSQIHALIEANDALPEVAPTEPDEIELAIGKHVAGLV
                     EDGATLQMGIGAIPNAVLSCLTGHKKLGIHTEMFSDGVIPLVEKGVITGEMKVKHPGK
                     IVSGFVMGTRKLYDFIDDNPFVSMLDIAYVNDTAVIRRNPKVTAINSAIEVDLTGQIC
                     ADSIGHRQFSGVGGQMDFVRGASLSEGGKPIIALPSQTSKGESRLVSCLKPGAGVVTT
                     RAHVHYVITEFGVADLYGKNLRQRARALIGIAHPSHRDRLTEEARKVLGI"
     gene            1319..3004
                     /gene="ilvB"
                     /locus_tag="PKK76_05330"
     CDS             1319..3004
                     /gene="ilvB"
                     /locus_tag="PKK76_05330"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNL67923.1"
                     /translation="MKQIRRQSLGKGDCVKSGARIILDYLEQESVPFVAGMPGGANLP
                     LYDALHSSSIVSILARHEQGAGFIAQGYARATGKVGVCFATSGPGATNLITSLADAKL
                     DSIPIIAITGQVPRDLIGTDAFQEVDTIGIARPGVKEAFFARDAQSLYDILPAAFAAA
                     RSGRPGPVLIDVPKDVQREVAAGRYAIMEPSRGTLSSASVEEAARMIQASRRPVLYAG
                     GGIIHSNASEILIQIAERNAIPVVTTLMGIGSIPTSHPRFFGMLGMHGEPYTNLLLEE
                     CDLLIAVGSRFDDRATGKLSEFCPDAKVIHVDIDRTELGKLRKPDLAIQADARTFLSA
                     LRNILPFHERREWTALCDDLRRDHPSENHDGNSEALSYLREISDSISDHDVITTDVGQ
                     HQMWAAQVCRIREPRTFLTSGGLGTMGFGLPAAIGAALGTGKRAVCISGDGSILLNVQ
                     ELALLADLNLDVKVVVFDNANLGMVRQQQQMFYGQRYSSSRFERAVDFVSLARSFGIP
                     GYSASDRPISSILREPGPALIHLPIDENENVYPMVAPGSANRDMIRRPAGKNS"
     gene            3001..3726
                     /locus_tag="PKK76_05335"
     CDS             3001..3726
                     /locus_tag="PKK76_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNL67924.1"
                     /translation="MKASFLGTYQGMRLFLLAPLLVVSCVSSSTYENDMKAKNAEISR
                     LSEEKAALAKKVQETETKARARSADIEVSRTQFKALADAGNLKLKLVDGRVVVVLSAD
                     VLFSTGSAELSDKGKAAVSDVAKVCATMKDKRFQVEGHTDNVQINPVPGFPYGDNWEL
                     GSARAVSVVNVMIAAGVNPDRVSAASFADTRPIASNDQPEGRAQNRRIEIILTPDLSQ
                     VPGFEYLKEAPPADTKPGEPKPQ"
     gene            complement(3720..5789)
                     /locus_tag="PKK76_05340"
     CDS             complement(3720..5789)
                     /locus_tag="PKK76_05340"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNL67925.1"
                     /translation="MQLKDPLYGLRILADCRKLRATVEKNTRNPDLDLLKRAFQFANR
                     AHREQRRLSGEPYIIHPLAVGVLLAEQGLDNATICAGLLHDVVEDTAFGEETIQSEFG
                     SEIHALVKAVTKISNVKKDRARAMGMAGPEIKRLKEREAAENIRLMLLATARDVRVIL
                     IKLADKLHNMRTLQFQKPEKVERIANEVMQIYAPIAGRLGMFRVKSELEDLAFMRLNP
                     EGYRVILEGLKQSRGDLEDFLETVRKILKRRLSEIKIEARIDGRAKHMYSIHQKMMNQ
                     DKTLGQIYDLRGIRIIVEEIRDCYGALGIVHTLWTPIHGRFKDYVATPKVNGYQSLHT
                     TVLGPDGRPLEIQIRTRDMDERAEYGIAAHWLYKSGIDGAERHRWLQRLTGLTEYVGD
                     TGEFIEDLKQELDAEEVYVFTPKGDIIDLPVGSTVLDFAFRIHTHVGLRCKGARVNDR
                     MVPLRTELKSGDRVEVLTDKNATPSPNWLRYLQSPRASQKLRAYLRKQQDDEDVQPPE
                     TPTEGTNDSNNVAELRIRRAKTTDPRRVPIEVAGENDIPVRYAGCCTPVPGDRIVGFI
                     TRGRGVTVHRVDCPNLAGESLEKDRLIQVRWEGLTEKYPVQIDIHARDRQGLYLDLVG
                     EITKTHTNILKAEADLPRSQGDLMRARFLIEVEHVDHLRDIIDSVMSVPGVVSVERMQ
                     DAKKQPH"
     gene            5892..6542
                     /locus_tag="PKK76_05345"
     CDS             5892..6542
                     /locus_tag="PKK76_05345"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNL67926.1"
                     /translation="MAQRSLFFNSLDDALEELSTIERFKAVDTTGLWSYYQILTHLAD
                     YLESSMNNFSWMQPLSMRRTIGKANFFKLKQAGKMDPGLANPSTPKVREEGDEKAALQ
                     RLRRMIEEYKKFVGEHPEHPFYGLLTREDWDFLHRIHMAHHLGFVREAKPKPQAKAKP
                     APKSAGKKPAASVKKSSTGKAKKSAKPAKKSAKKTAKKPAAKSKPAKKAAKKAKKR"
     gene            complement(6571..7392)
                     /locus_tag="PKK76_05350"
     CDS             complement(6571..7392)
                     /locus_tag="PKK76_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67927.1"
                     /translation="MRWQSAEVTAAAPSPWKDLPESPISFLVEVNGSNIVMVPGINDA
                     PACILTVEEVRLPSTWTLIRRITSIPATVVARMVQPFLPVENTNQTLCDILGWKRDYQ
                     NLTEAADLLSFDSQTIQLSCMHDLPVPVLRLFARIQEKTQLLQIFKTRSVRKNLVREI
                     ILDLYDLEEGARTRVINELLRSAQSTDSLAGEDLRDLVRKERHPKSEQIRQQIKEIKK
                     SMNLPSGLQIEVPADLENGQTKITLSFRRQEEFQKLLGEMDQAFSAKLSEILKLV"
     gene            complement(7392..>8586)
                     /locus_tag="PKK76_05355"
     CDS             complement(7392..>8586)
                     /locus_tag="PKK76_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNL67928.1"
                     /translation="RQYLDFYLSGMMYNQYTEQEIALDLSKKVRKYLELTRSDDGWLE
                     FEKILRLFSDQKDFERVRFKIDMAREDGWQNRGKPVTDPEVNAPYRMYDYPNPARDMR
                     MEMGRVSYINSKQPYLVLALHLNPAEGTNPGGMGAVLSPGYPTFDLLRRITLGQAPAD
                     AFYKLPWADKWLISDPGFTQFESAKGDAWVYFHGYRMLKNNTPWLGKNRGIRWNMITW
                     KYSDPPGWEKLAAKAGPGPYAIKYAEWKPEGKFWDRERSPMELWRHEGGPLGYGGDNH
                     YASDELLRFVQMGVPIQVPERRAPGSMGEIVPPFVSTYGLPTFSNAIIAYLEIGHLNR
                     DRDRTMVLQNSEELARSLAAGIYSLFRGLSVKKTGKVRPKGAPLNFAKYENAPGGNYF
                     RAVTE"
BASE COUNT         1988 a         2157 c         2254 g         2187 t
ORIGIN      
        1 gagcctccga actgcgtgac gtagagctgg tgcacataca cacggaggga ggtgctccct
       61 atgcgaagcc ggagttcgcg gccagtttca aaaccaatgt cttttttgtc ggaggcaaca
      121 ttcgagaagc ggttcagaat cacagagcgg attacgttcc agtctttctc agtgagatcc
      181 ccgcactttt cagaaaaaag attctgccca ttgacgtggc catgattcat gtttctcctc
      241 cagacaatca tggatactgt tccctgggtg tttccgtaga catcgcacgc gccgcggttc
      301 agtgcgcggg taaaatcatc gcacaggtga atcctgccat gcccagaact cacggcgaaa
      361 gcatggtcca cgtcagccag atccatgctt tgatagaagc gaatgatgca cttccggaag
      421 tagcccctac agaaccagat gagatcgaac tggccattgg caaacatgtg gccggtctgg
      481 tggaagatgg agccactctt caaatgggaa ttggtgccat tccaaacgca gtgctctcct
      541 gtctgactgg tcacaagaaa ctgggaattc atacggaaat gttttcagac ggagtcatcc
      601 ctctcgtcga gaaaggagtg attacgggag aaatgaaggt gaagcatccc gggaagatcg
      661 tttccgggtt tgtaatgggc actcgcaagc tgtacgattt catcgatgac aatccgtttg
      721 tgtccatgct ggacattgcc tatgtcaatg acactgcagt gattcgacgt aatcctaaag
      781 taacagcgat caacagtgcc atcgaagtgg atcttacagg tcaaatttgt gccgattcga
      841 ttggtcatcg tcagttttcg ggagtaggag gccagatgga ctttgttcga ggcgcttctc
      901 tctctgaagg cggaaagccg atcatcgcac ttccgagcca gacatccaaa ggagaatcca
      961 ggctcgtcag ttgtttgaag cctggcgcgg gtgtcgtgac cactcgtgcg catgtgcact
     1021 acgtgattac ggagtttggc gtggctgatt tatatggaaa aaaccttcgc cagagggcgc
     1081 gcgctctcat cggaattgct catccatcgc accgtgatcg cctgacggaa gaagcccgta
     1141 aggtgctggg catttaaaaa atatagatca aagccaggaa tttgaaagac gattcctggc
     1201 tttgtagttt tcgtggcttc aaatgtcccg cgcaatgaat acactggtcc tggtcctcgt
     1261 ggtcgtagta ttcatttctg cgtcgtaagg acacgatccc gtcggcgcag ccggcgggat
     1321 gaaacaaatc cgccggcagt ccctcggaaa aggagactgt gtgaagagcg gagcaagaat
     1381 catcctcgac tatctggaac aagaatccgt gccttttgta gccggaatgc ctggaggagc
     1441 caatcttcca ctctacgatg cgctgcattc tagctccatc gtgtcaattc tggcaaggca
     1501 cgagcaggga gcggggttca ttgcgcaggg ctatgccagg gccacaggca aagtgggtgt
     1561 gtgctttgct acttccggcc cgggtgccac caatctgatc acttccctcg cagatgcgaa
     1621 gctagattcg attcccatca ttgccatcac aggtcaggtg ccgcgagatt taatcggaac
     1681 agatgcattt caagaagtgg atacgatcgg gattgcaaga ccaggggtaa aggaagcgtt
     1741 ctttgcgcgc gatgcacagt ctctctatga cattcttcct gcggcttttg cagctgcgcg
     1801 aagcgggagg ccaggacccg ttctgattga tgttcccaag gacgttcaga gggaagtggc
     1861 ggccggacgt tatgcgatca tggaaccatc gcgaggaaca ctttccagcg cttccgtgga
     1921 ggaagcggcc aggatgatcc aggcttcgcg acggcccgta ctttacgccg gtggaggcat
     1981 cattcattcg aatgcatctg aaatcctgat tcaaatcgca gaaagaaatg cgattccagt
     2041 tgtgacgact ctcatgggca ttggatcgat tcccacatcc catcccagat ttttcggaat
     2101 gctcggaatg cacggagagc catacaccaa tctccttttg gaagaatgcg acttgttgat
     2161 tgcggttggt tctagattcg atgatcgagc cacaggaaag ctttccgagt tttgtccgga
     2221 cgcgaaggtg attcacgtgg acattgacag aactgaactg ggcaaactca gaaagccgga
     2281 tctggccatt caggcggatg ccagaacgtt tttgtctgcc cttcgaaaca ttctgccttt
     2341 ccatgagcgc agagaatgga ctgcactgtg cgatgacctt cgccgcgacc atccatctga
     2401 aaaccatgac ggaaattcag aagctctttc ctacttgcgc gagatttctg attcgatttc
     2461 cgaccatgat gtgatcacta ccgacgtggg tcagcaccag atgtgggctg ctcaggtatg
     2521 cagaatcaga gagccgcgca catttttaac gtcaggcggc cttggaacca tgggctttgg
     2581 tcttccggct gcgataggtg ctgcacttgg aacaggaaag agagcagtgt gtatttccgg
     2641 tgatggttcc attttactta acgtacagga gcttgctctt cttgcggatt tgaatctgga
     2701 cgtgaaggtt gtagtttttg acaacgcgaa cctggggatg gtgcgtcagc aacagcagat
     2761 gttctacggc cagaggtact catcttccag gttcgaacgt gcggttgatt tcgtatctct
     2821 tgcgagaagt ttcggaattc caggatattc tgccagtgac cggcccatct ccagcatttt
     2881 gagagaacca gggcctgcgt tgattcatct tcccattgat gaaaatgaga acgtgtatcc
     2941 aatggtggcg ccgggaagcg cgaatcgaga tatgatcagg cgacctgcgg ggaaaaacag
     3001 ttgaaagcct cgttcctcgg gacatatcag ggcatgcgac tgtttctact tgcgccgctt
     3061 ctggtggtat cgtgcgtttc ttcatctact tatgaaaatg acatgaaagc aaagaacgct
     3121 gagatcagca ggctttcaga ggaaaaagcg gctctcgcaa agaaagtgca ggaaacggaa
     3181 acaaaggcca gagcgcgaag tgcggacatt gaagtatctc gcactcagtt caaagctctc
     3241 gcggacgctg gaaatctgaa gctgaagctt gtagacggaa gagtcgtggt cgtgctgtcc
     3301 gcggatgtac tgttctcaac cggctctgca gagctctcag ataaaggtaa ggccgctgtc
     3361 tctgacgtcg caaaagtgtg cgccaccatg aaggacaagc gattccaggt agaaggtcac
     3421 acggacaatg tgcagatcaa ccctgtgcct ggctttcctt acggagacaa ctgggagctg
     3481 gggtctgcgc gagcggtcag tgtggtcaac gtcatgattg cggctggagt gaatcctgac
     3541 cgggtgagcg ccgcgagttt cgcggacact cgtccgattg cgtctaacga tcagccggaa
     3601 ggacgcgcgc aaaatcgaag gattgaaatt atacttacgc cggatctttc gcaggttcca
     3661 ggatttgaat acttgaaaga agctccgcct gcggacacga aaccaggcga accaaagcct
     3721 cagtgaggct gctttttcgc gtcctgcatt ctttcgacag acacaacacc tggcacggac
     3781 attacgctat ctatgatgtc gcgtaaatga tccacgtgtt ccacttctat gaggaagcgc
     3841 gcgcgcatga gatctccctg tgagcggggt agatctgctt ccgccttcag tatgttcgta
     3901 tgagtttttg tgatttcacc caccaggtcc aggtacaggc cctgtctatc ccgtgcatga
     3961 atatcgattt gaaccggata tttttcagtg agtccttccc agcgtacctg gatgagtctg
     4021 tctttttcca gcgattctcc tgcaaggttc gggcagtcca ctcgatgtac tgtgactccg
     4081 cgaccacgag tgatgaatcc gacgattcta tctccgggaa cgggtgtgca gcaccctgcg
     4141 tagcggacag ggatgtcgtt ctctcctgcc acttcaatgg gaacgcgtct cgggtcggtt
     4201 gttttcgcgc gccgaattcg cagttccgca acattgttcg aatcgtttgt tccttccgtt
     4261 ggagtttcag gcggctgcac gtcttcatca tcttgctgtt tgcgcaggta tgcccgcagc
     4321 ttttgcgaag ctcgcggaga ctgaaggtat ctgagccagt tcggagaagg ggttgcgttt
     4381 ttgtccgtga gtacttctac gcgatctcca ctctttagtt cagtgcgaag aggaaccatg
     4441 cgatcattga ctcgcgcgcc cttgcatctg agaccaacgt gtgtgtggat gcgaaatgca
     4501 aaatcaagca cagtcgaacc aaccggtaga tcgatgatgt cacccttcgg agtgaacacg
     4561 tacacttcct ctgcatcgag ttcttgcttg agatcttcta tgaattcgcc tgtgtctccc
     4621 acatattccg tgaggccggt caagcgctgc aaccagcgat gtctctctgc tccgtctatt
     4681 ccactcttgt acaaccagtg cgccgcgatt ccgtattccg ctctttcgtc catatcgcgc
     4741 gtgcgaattt gaatctccag gggacgtcca tccggaccaa gtacggttgt atgcagactc
     4801 tggtatccgt ttactttcgg tgtggccacg tagtctttga atctaccatg aatgggagtc
     4861 cagagtgtat gaacgatgcc cagcgcaccg taacaatctc tgatttcttc tacgatgatg
     4921 cgaatgccgc gcaaatcata aatctgaccg agagtcttgt cttgattcat catcttttga
     4981 tgaatggagt acatgtgttt cgcacgtccg tctatgcgcg cttcgatttt gatttcgctc
     5041 agtcggcgtt tgagaatttt gcggacggtt tcgaggaaat cctcgagatc cccacggctt
     5101 tgtttgagtc cttctagaat gacccgatac ccttccgggt tgagtcgcat gaatgcgaga
     5161 tcttcgagct ccgatttgac tcggaacata ccaagtcgtc cggcgattgg tgcatagatc
     5221 tgcatgacct catttgcaat gcgttcaact ttttcaggtt tctgaaactg cagtgtgcgc
     5281 atgttgtgaa gtttgtctgc aagcttgatc aggatgactc gaacgtctcg cgcggttgcc
     5341 agaagcatga ggcgaatatt ttccgcagct tccctttctt tgagcctttt gatttccggc
     5401 cctgccattc ccatggcccg cgcccgatcc ttctttacat tagaaatttt agtaactgct
     5461 tttaccaggg cgtggatctc agagccgaat tcagactgaa tggtctcctc tccaaaagca
     5521 gtgtcttcta ctacgtcatg gagtagcccc gcgcaaatgg ttgcattgtc cagtccctgt
     5581 tcggcgagaa gcacgccgac tgcgagcggg tggatgatgt aaggctctcc ggagagtctt
     5641 ctttgctctc tgtgtgccct gtttgcaaat tggaaggccc tcttcagcag atcgaggtcc
     5701 ggatttctcg tatttttctc cactgtggcg cggagttttc ggcaatcggc cagaattctg
     5761 agcccgtata gcggatcttt cagttgcatg gccgtcttct ggtgaacata taagatgttt
     5821 tcattctatt caaccagaaa aatgctatat ggctggacgc agttctatcc aatgggccct
     5881 taagaattgc tatggcccag agaagccttt tttttaacag cctggatgac gctctagagg
     5941 agctttccac tatagaacgt ttcaaggcgg tggacacaac cggtttgtgg tcctattatc
     6001 agatactgac gcacctggcg gattatctgg aatccagcat gaacaatttc agctggatgc
     6061 agcctctgtc catgcgccgt accatcggaa aggccaattt ctttaaactg aagcaagcgg
     6121 gtaagatgga tccggggctt gccaacccgt ccactcccaa ggtgcgcgag gaaggggacg
     6181 aaaaggcggc gctccagaga ctgcgtcgca tgatcgaaga gtacaagaaa ttcgtgggag
     6241 agcatccaga gcatccgttt tacgggctcc tgacgcgcga agactgggac ttcctgcatc
     6301 gcattcatat ggcacatcat cttggatttg tgcgcgaagc aaaacccaaa ccgcaggcaa
     6361 aagcaaagcc ggctccaaaa tctgccggca agaagcctgc cgcttccgtc aaaaaaagct
     6421 ccactggaaa agcaaagaaa tcggcaaagc ctgcgaagaa aagtgccaaa aagactgcga
     6481 aaaaaccggc cgcaaaatct aaacccgcga agaaagcagc gaagaaggct aagaaacgct
     6541 gacggtcttc gcggcgcatc gccgctcgtc ctaaaccagc tttaggattt cagagagttt
     6601 cgcgctgaat gcctggtcca tctctcccag gagtttttga aattcttcct ggcggcgaaa
     6661 tgatagcgtt atctttgtct ggccgttttc aagatccgcc gggacctcta tctgaaggcc
     6721 ggaaggcaga ttcatcgatt tcttgatttc cttgatctgc tgtctgattt gttctgattt
     6781 tggatggcgt tccttgcgca ccagatcccg gagatcttct ccagccagtg aatctgtgct
     6841 ctgtgcggac cggagtaatt cgtttatcac acgggtgcgg gcgccctctt ccaggtcgta
     6901 cagatccaga atgatttcgc gcacaaggtt ctttcgtaca ctgcgagttt tgaatatttg
     6961 aagcaattga gttttttcct ggattctggc aaagagtctg agcacaggca caggcagatc
     7021 gtgcatgcag gatagctgaa tagtttgaga atcaaatgag agcaagtccg cggcttctgt
     7081 caggttctga taatctcttt tccagccaag aatgtcgcat aacgtttgat ttgtattctc
     7141 aacgggcaga aatggctgaa ccattctggc aacgacggtc gctgggatag acgttattcg
     7201 acgaatcagc gtccaggtgg acgggagtct gacttcctcg actgtcagaa tgcaagcggg
     7261 tgcgtcgttt attcctggga ccattacaat attggaaccg tttacttcca ccaggaaaga
     7321 gataggagat tcaggtagat ctttccacgg agagggtgca gccgcggtaa cctcagctga
     7381 ttgccagcgc attattctgt gactgcgcga aagtaatttc cgcccggagc gttttcatac
     7441 tttgcaaaat tcagaggagc gcccttaggc cttaccttgc ctgttttttt gacagatagg
     7501 ccgcgaaaga gagagtagat gcccgccgcc aggcttcgtg ccagctcttc cgaattttga
     7561 agcaccatgg ttctgtctct gtcccggttg agatgcccga tttcaagata agcgatgatt
     7621 gcattggaaa aagtaggcag tccatatgtg ctcacaaaag gcggaacaat ctctcccata
     7681 gaccccgggg cgcgtctttc tggaacctgg atgggcacac ccatttgaac gaatcgaagc
     7741 aattcatcgc tagcgtagtg attgtctcct ccgtatccaa gagggccgcc ttcatgtctc
     7801 cacagttcca tcgggcttct ttcgcgatcc cagaactttc cttctggttt ccattctgcg
     7861 tatttgatag catatgggcc tggtcccgct ttagctgcga gcttctccca gcccggagga
     7921 tctgaatact tccacgtaat catgttccag cgaattccgc gattcttgcc cagccacggg
     7981 gtattgttct tgagcattct atacccgtga aagtagaccc acgcatctcc ctttgctgat
     8041 tcaaactgag tgaaacctgg atcgctgatc agccatttgt ctgcccaggg aagtttgtaa
     8101 aatgcatctg ccggcgcttg ccccagtgta attctgcgca atagatcaaa cgttggatac
     8161 ccgggagaaa gaacagcacc cattccacct ggattggttc cttcagccgg atttagatga
     8221 agagcaagaa cgaggtacgg ctgcttgctg ttgatatagg atactcggcc catctccatg
     8281 cgcatatcac gcgccgggtt cgggtagtca tacattcggt acggagcgtt cacttcagga
     8341 tctgttacgg gctttccgcg gttctgccat ccatcttcgc gcgccatgtc gattttgaat
     8401 cggacgcgct cgaaatcttt ctgatccgaa aacagtcgta agattttttc aaactcaagc
     8461 cagccatcgt ccgatcgcgt gagttccagg tacttgcgga ctttttttga aagatccaga
     8521 gcgatttctt gttccgtata ctgattgtac atcatcccgg aaagatagaa gtccagatac
     8581 tgacgc
//
LOCUS       DAORFF010000089         8546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_3990_length_8546_cov_3.591568, whole genome shotgun sequence.
ACCESSION   DAORFF010000089 DAORFF010000000
VERSION     DAORFF010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3990_length_8546_cov_3.591568"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1121)
                     /locus_tag="PKK76_05360"
     CDS             complement(99..1121)
                     /locus_tag="PKK76_05360"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNL67929.1"
                     /translation="MLPINVDFLGLHFRGYEGHWAFATLVLGFFYQRAKSLRAGYSND
                     WFVSAYTFAILTGFVCARLFHFMFWDTTNFLANPLIVFRPGGGFAILGGTIGTGFGGW
                     LYCKRTGANFYHWCESLMTPICLCLALGRISCFLNGDAYGLPSTLPWAVSFSEKSLDW
                     MAEWNGLHHLYANSDQPLAVISSIFQQYVPLSALPLPQSLQHLQAEGVHSVADLARFY
                     PPNALLSQSELAAKGLVPFPTVYPRVHPAQLYELGIMLVAYFFIMYAEKQEWARQRSF
                     FLFWICYGLNRFFIEMVRGDRNVAFASLTYAQVISILLVISGAGAFYYLTRKWKQTGL
                     PEPVLK"
     gene            complement(1130..1558)
                     /locus_tag="PKK76_05365"
     CDS             complement(1130..1558)
                     /locus_tag="PKK76_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNL67930.1"
                     /translation="MSLSEDLYKEVILQHAGAPSHRGPLVGANVKESGVNRSCGDELE
                     LELIVKDGKIEKIALGGKGCSIHTASGSLMAEAVEGKSIAEALHIIRDFKSMLVDQKD
                     IHFPEDLEELEALRGVQRYPIRVKCATLSWNTLEQALNHK"
     gene            complement(1631..2353)
                     /locus_tag="PKK76_05370"
     CDS             complement(1631..2353)
                     /locus_tag="PKK76_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose-lipid carrier transferase"
                     /protein_id="HNL67931.1"
                     /translation="MSVLRFKDLDNSVSLERPEYKASLNDLQEKVRLLSHQAADENRS
                     IIVVFEGWDAAGKGGAIRRLTASLDPRLFRVNSIAAPNDIERKHHYLWRFWTRLPQLG
                     YMSIFDRSWYGRVLVERIEGFAKQDEWSRAYQEINEFERELTVSGAIILKYWLHITPE
                     EQKRRFTERQNDPLKRWKLTDEDWRNREKWPEYEQAAEEMFLKTGTASCPWTLVPAND
                     KYHARIFILDDFVRRLERELSA"
     gene            complement(2350..3102)
                     /locus_tag="PKK76_05375"
     CDS             complement(2350..3102)
                     /locus_tag="PKK76_05375"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67932.1"
                     /translation="MIRLRDYETGPELEKDSYDAAEKDLQERLYILFNECANRGLSCL
                     TILEGWAASGKGDALKTMTVRLDPRKFIVYTEGRGNSGYPFMYPYWQSLPYYGKAQFL
                     IGSWYSRAVDRHVKGKDSKQVFSENLESISNFERTICADNYTIFKFFLNISRDTQKSR
                     MKRAKKQGLSWIVARGDAKQNDRYAEYMEHYERILDKTDAGVSPWNIIAAENKYYTYF
                     TIMNTMVKSLEARLGVDSAAVMGQMKRTEEFA"
     gene            3228..4169
                     /locus_tag="PKK76_05380"
     CDS             3228..4169
                     /locus_tag="PKK76_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNL67933.1"
                     /translation="MRIPEAPGRKSRALIVEGGGMRGSFAGGVLASMSRIYPASNFDI
                     VVGVSAGSCSAAYYVASTPAERLVEFCLDVWRYELTGSKFISYWNVLKGKTILNQEYL
                     IDYLFGKKYPIPKGRMDEPDVTPCYVTVTNLHTFLPEYVRATSSNLLSLLKAATSLPI
                     ATRGRKRLGEHEYTDGAVLDPMPIEAVMEAGYKDITVVLNHPRSHRSDPVGKMLSWLS
                     FPGNKTIRQMISREHHIRFNRAFDLINHPPTGFKVNIVDPDKPVAAGLVTRDMGRLNR
                     TVDHGIETGLRYFSELQKSRVFARFRDFFTRSSSGSR"
     gene            4166..4987
                     /locus_tag="PKK76_05385"
     CDS             4166..4987
                     /locus_tag="PKK76_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNL67934.1"
                     /translation="MRLFGNWKFGRRPRIGVALGSGAARGWAHIGVLRCLEMNRIPVD
                     VVAGCSAGAVIGAFYAARAMTQVEKFALMYKGVRDTFKYLDFSPRSGGLVQGKNFIKF
                     LEEHLPVRNFSELKMPFGVVATDLVHMEEVHISDGPLLPAIRASVAVPGFLSPLESRS
                     PAGEVRQLVDGGLLNPVPVSLCRKLGADIVIAVDLNSEKFHEPAKSMTDIMARSIDAM
                     REVHKRNNFNFYPADVLVEPHMPEFGFFDYHRTQEAIDIGSKAAEEKLDRIKRLL"
     gene            complement(4971..5882)
                     /locus_tag="PKK76_05390"
     CDS             complement(4971..5882)
                     /locus_tag="PKK76_05390"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNL67935.1"
                     /translation="MANFSDSPFRKICRRMGSALSFSEFVPANSIAAGRPWALDMLRF
                     EEMERPVILQIFGNKLRSIEEAAIAVLERRPDVIDLNMGCSAREVALNGSGAGLLRTP
                     ELASAMIRSLVRIAGPEIPVSAKMRIGWDGFSRNYRDIALMIEDSGASMISVHGRTRE
                     EGYGGIADWDAIAEVKSMVRIPVIGSGDVSSRLDAENRIAQFGVDGVLVGRAAMGNPW
                     IFSGIRRSTLPREQITSVIKEHFLDMLTFYGDHAVILFRKHATRYAAEFPQHERLALL
                     SCETPSEFLDRLEECAQAEKLEATAIA"
     gene            5964..6260
                     /locus_tag="PKK76_05395"
     CDS             5964..6260
                     /locus_tag="PKK76_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67936.1"
                     /translation="MRAANVFRYVLPIQSSGHLSGAIVTWMLDRNRIEFTFDEFREMT
                     AAIHSGGLFKYLAGERPALLSQLTSIVRDKVPDIEEVEMEFVLEQALLNLQSRL"
     gene            6320..7825
                     /locus_tag="PKK76_05400"
     CDS             6320..7825
                     /locus_tag="PKK76_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL67937.1"
                     /translation="MGETNAQFRQILDVSPIPFALNDENQNITYLNRAFTRTFGYDLT
                     DIPTLAEWWPRAYPEPAYRERVMKSWQERMEKATREGTQFEALELTIRCKDGSDRIVI
                     VSAVSPGDAPEGVYAVMLYDITERMNLLAAAQRADRLNSLGVLAGGIAHDFNNLLSSI
                     FGYMELARGATTESRVIEYLDRGLSAFSRARGLAQQLLTFARGGDPVMQSGNLGTLLS
                     ETCEFVLAGSNVHYQCHVADDLWPCNFDTGQMNQVFSNLLINARQAMPDGGNIGAVAS
                     NRSLNETSVHPAGPYVEIQISDSGRGIPAEQISHIFDPFYSTRPGGTGLGLATCFSII
                     QKHAGFIDVESTPGKGATFRILIPVAKSGNSRGASFAKHSAGEQRLLVMDDEELIRAV
                     SQDLFASAGFSVQVAAHGEEALILCDEAARSGRPFHAAILDLTVPGHMGGKDLARLLR
                     KDHPEIALFASSGYSEDPVMTAPAEYGFVASIRKPYLIADAAQLVRQVLRT"
     gene            7788..8351
                     /locus_tag="PKK76_05405"
     CDS             7788..8351
                     /locus_tag="PKK76_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNL67938.1"
                     /translation="MQRSWFARFFALSVGLTKSGSGANNDLKDRRFMKLSKKTDYALR
                     AIFTLVEQHATGEPVSISDLAQANRIPKRFLEHIMLDMKSQGWVSSTAGKYGGYRLAK
                     HPSQITMGEIVRYFDGILAPIGCVSIARYEPCKQERVCRFRRLLLNVRNAVAGMMDRA
                     TLESVFAEGIVTQKEVMHVSLMGGDGI"
BASE COUNT         2069 a         2087 c         2183 g         2207 t
ORIGIN      
        1 ctgtgacggg ggcgaacgca ggatcagctg cgaggagttt caatacttcg gaaccaatca
       61 gtccagttcc accaactacg atgcttccat ttgccatatt atttcaacac cggctctgga
      121 agtccggtct gtttccattt ccttgtaaga tagtagaacg cgccggcccc tgagatgacc
      181 agcagaatcg aaatcacctg agcatatgta agcgatgcga aagcaacgtt tctgtctccg
      241 cgaaccattt caatgaagaa gcgattgagt ccgtaacaga tccagaataa aaagaaagaa
      301 cgctgtctgg cccattcttg cttctctgcg tacatgatga agaagtaggc gaccagcatg
      361 atgcccagtt cgtagagttg tgcaggatga acgcgcggat acacagttgg aaatgggaca
      421 agaccctttg ccgccagttc agactgagag aggagagcat ttggcggata gaaacgagcc
      481 aggtctgcta cggaatgaac gccttccgct tgcagatgct gcagagattg aggaagagga
      541 agagcggata acggcacata ttgttggaat atggaagaaa tcactgcaag cggttgatcg
      601 ctgtttgcat acaggtgatg caggccattc cactctgcca tccagtccag actcttttct
      661 gagaaactga ctgcccatgg aagtgtgctt ggcagtccat acgcgtcccc gttcaagaaa
      721 cagctgattc tcccgagcgc caggcacaaa caaatcggag tcatgagtga ttcgcaccag
      781 tggtaaaagt ttgcgccggt gcgcttacaa tagagccacc cgccgaaccc tgttccgatg
      841 gttcctccaa gaatggcaaa tccaccaccc gggcgaaaaa caatcagcgg attggcgaga
      901 aagttggtgg tatcccagaa cataaaatga aagagtctcg cgcaaacaaa tccagtcaat
      961 atggcgaatg tgtaagcgga tacaaaccag tcgttggaat aacccgcgcg aagagacttt
     1021 gctctttgat agaagaaacc cagcacaaga gtggcgaaag cccagtggcc ttcatagcca
     1081 cgaaaatgaa ggcctagaaa atctacgtta atcggaagca tggtcctgcc tatttatgat
     1141 tgagcgcctg ttcgagcgtg ttccaggaca gagtggcgca ttttactctt attggatatc
     1201 tttgcacacc gcgcagtgct tcgagttcct ccaggtcttc cgggaagtgg atgtcttttt
     1261 gatctacgag catagatttg aaatcgcgga tgatgtgcaa tgcctcagcg atcgactttc
     1321 cttccactgc ttccgccatg agcgatccag aggcagtgtg gatgctgcat ccttttccac
     1381 ccagagcgat tttttctatt ttcccatcct taacgatgag ttccaactcc agttcgtctc
     1441 cgcagctgcg attgactccg gattctttca cattggcgcc cacaagcgga ccgcgatgag
     1501 agggcgcgcc cgcgtgctgc aaaattactt ctttgtaaag gtcctcggac agtgacatcc
     1561 tacagttaac aaaatcttaa gcccgcattc ggcaactgat tctcgaatga ctgattctcg
     1621 aatgtcacac tcaggcagag agttctcgct ctagcctccg aacgaaatcg tctagaatga
     1681 agatccttgc gtggtattta tcattggcag gtacaagagt ccaggggcag cttgcggttc
     1741 ctgttttgag gaacatttcc tctgctgcct gctcatactc gggccatttt tcgcgattgc
     1801 gccagtcttc gtctgtaagc ttccagcgct tgagcggatc gttctgtcgc tccgtgaatc
     1861 ttctcttttg ctcttccggt gtaatatgga gccagtattt gaggatgatg gcaccggaca
     1921 ccgtgagttc tctttcgaac tcattgattt cctgatacgc gcgagaccat tcatcctgct
     1981 tcgcaaaacc ttctatgcgt tccacgagaa ctcgtccgta ccacgatcga tcaaaaatgc
     2041 tcatgtatcc gagctgcggc aagcgcgtcc agaatcgcca caggtagtga tgctttcttt
     2101 ctatatcatt gggcgcggca atcgaattga ctcgaaacag tctcggatcc agggaagcag
     2161 tcagcctacg aatggccccg ccttttccag cggcgtccca accttcgaat actacgatga
     2221 tggatctatt ttcatccgca gcctgatggg aaagtaggcg aactttttct tgaagatcat
     2281 tcagacttgc tttgtactca gggcgttcca gggaaactga attgtccagg tctttgaatc
     2341 gaagtacgct cacgcaaatt cctccgttct tttcatctgg cccatgacgg ctgcggaatc
     2401 taccccaagg cgtgcttcga gagattttac cattgtgttc atgattgtaa agtatgtgta
     2461 gtatttgttt tccgcggcta tgatgttcca gggagagaca ccggcatccg tcttgtccag
     2521 gattctttcg tagtgttcca tgtattccgc ataacggtcg ttttgctttg catctccgcg
     2581 tgcgacaatc cagctcagcc cctgcttttt ggctcgtttc attctggatt tctgtgtgtc
     2641 gcgagaaatg tttagaaaga atttgaaaat tgtgtaattg tccgcgcaga ttgttctttc
     2701 aaaattggaa atagattcca ggttctcgga gaagacttgt ttagaatctt tacctttcac
     2761 gtgtctatcc accgcgcgac tgtaccagga tccaatcagg aattgcgctt ttccgtagta
     2821 agggaggctc tgccagtacg gatacatgaa cggatagccg ctattgcctc tcccttctgt
     2881 gtagacgatg aactttcgcg ggtccaaccg cacagtcatt gttttgagag catctccctt
     2941 gccgctggcg gcccagccct ccaggattgt cagacacgac agtccgcgat tggcgcattc
     3001 attgaataga atatagagcc tttcttgcag atctttttct gccgcatcgt aggaatcttt
     3061 ttctagctct ggtccggttt cgtagtcgcg caggcgaatc atgaccgaga attgatggtc
     3121 cggggaatat ctgtcaaaca caaaggccgc gaattcgaga ctcaaaagta ctggatttta
     3181 aagggcgctt tccgaccata tggttagaag agaattctga atcaaacatg agaatacctg
     3241 aagcacccgg gcgaaagtca cgagcgttga ttgtggaagg cggaggaatg agaggttctt
     3301 tcgcaggcgg cgttcttgcg tctatgagca gaatttatcc tgcatccaac ttcgatatcg
     3361 tagtaggagt atccgcagga agttgctctg cggcgtatta tgtagcatcc actcccgcgg
     3421 agcgcctggt ggagttctgt ctggacgtat ggagatatga gcttaccgga agcaaattca
     3481 tttcttactg gaacgtgctc aaaggtaaga ccattctaaa tcaagaatac ttgatcgatt
     3541 atttatttgg aaaaaagtat cccattccaa aaggaagaat ggatgaaccg gacgtgacac
     3601 cctgttatgt gacagtcacc aatcttcata cgttcttacc tgaatacgtt cgcgctacga
     3661 gttcgaattt gctgagcctg ctaaaggctg ccacttctct tcccattgca acacgtggta
     3721 gaaagagatt gggggaacat gaatacacgg acggcgcggt tcttgatcca atgcccatcg
     3781 aagcagtgat ggaagcgggc tacaaagaca tcacggttgt attgaatcat ccaagaagcc
     3841 atcgttcgga tcctgtagga aaaatgctca gctggttatc ttttcctgga aacaaaacaa
     3901 tcagacagat gatttcgcgc gagcatcaca ttcgcttcaa tcgcgccttt gatttgatca
     3961 atcatccgcc tacaggattc aaagtgaaca ttgtggatcc ggacaaacca gtcgcggcgg
     4021 gtcttgtgac tcgcgacatg ggaagattga atcgtaccgt ggatcatggc attgaaacgg
     4081 gcctccgtta tttttcagag ttgcagaaat cacgcgtctt cgcgcggttt cgcgatttct
     4141 ttacacgctc ttcatcaggc tcaaggtgag attgttcgga aactggaagt ttggacgcag
     4201 accgcgcatt ggcgtggccc ttggttctgg cgctgcccgc ggttgggcgc acatcggtgt
     4261 tttgcgttgt cttgagatga atcgcattcc tgtggacgtc gtggctggtt gttctgcggg
     4321 tgcggtgatt ggcgcttttt acgctgcgcg cgccatgacc caggtcgaaa agttcgcgct
     4381 gatgtacaag ggagtgcgcg atacattcaa gtatctggac ttttctccac gaagcggtgg
     4441 actcgtacaa ggaaagaatt ttattaagtt cctggaagag catcttcctg tgcgaaattt
     4501 ttcagagctc aagatgccgt ttggtgttgt tgcaacggat ctagttcaca tggaagaggt
     4561 tcatatttca gacggtccac ttcttccagc gattcgtgcg tctgttgcag tcccaggatt
     4621 tctatccccc cttgaatcac ggagccccgc cggagaagtg cgacagcttg tagatggagg
     4681 tcttctgaat ccggtgccag tctcgctctg cagaaaactg ggtgcggaca ttgtgattgc
     4741 tgtggacttg aattctgaga agtttcacga gcctgcgaag tcgatgacgg acatcatggc
     4801 tcgttccatt gacgccatgc gcgaagtgca taagagaaac aatttcaact tttatccggc
     4861 agatgtcctg gtggagccgc acatgcctga atttggattc tttgattatc acagaaccca
     4921 ggaagccatt gacattggat caaaagcagc ggaagaaaag ctcgatcgaa tcaagcgatt
     4981 gctgtagctt ccagtttctc cgcttgcgcg cattcttcca gtcgatccaa aaactctgat
     5041 ggcgtttcgc acgatagtag agccagccgt tcgtgctgcg ggaactccgc cgcataacgg
     5101 gtcgcatgtt ttctaaatag aatgactgcg tgatcgccat agaaagtaag catgtccaga
     5161 aaatgttctt tgatcacaga tgtgatctgt tcgcgcggaa gagtagatcg ccttatccct
     5221 gaaaagatcc agggatttcc catggccgct cttcccacca gcactccgtc tactccaaac
     5281 tgagctatac gattttctgc atcgaggcgc gaagacacat caccggaccc aattacagga
     5341 atgcgaacca ttgattttac ttccgcgatt gcatcccagt ctgcgatccc accgtatcct
     5401 tcttcgcgcg tgcgaccgtg aacagaaatc atgcttgccc cgctgtcttc aatcatgaga
     5461 gcaatgtctc tgtagttgcg cgaaaatcca tcccaaccaa ttctcatttt cgcactcact
     5521 ggaatttctg ggcccgcaat tcgaacaaga gagcgaatca tggcagatgc aagttcagga
     5581 gtacgaagga gccctgctcc tgatccgttg agtgcaactt cacgagccga gcatcccatg
     5641 ttcaggtcga tcacatcagg acgtctttct agaacggcaa tcgctgcttc ttcgatggaa
     5701 cgcagtttat ttccgaagat ctggagaatg accggtcttt ccatctcttc gaatcggagc
     5761 atgtccaggg cccatggtct tcctgctgcg atggaattcg cgggaacaaa ttctgagaac
     5821 gacagagcgg agcccattct tctgcaaatt ttgcgaaatg gactgtccga gaaattcgcc
     5881 ataggcgaaa ggatcatccg accgtaaatg gggactcgac ctatgtgaaa ggacggctgc
     5941 accggctaag aatcagttga gccatgcggg cggccaatgt ttttcggtat gtgcttccca
     6001 ttcaatcgag cggtcacctc agcggtgcga tagtgacatg gatgctggac cgaaaccgaa
     6061 ttgagtttac gtttgacgag ttcagagaaa tgaccgcagc gattcattcc ggcggtttgt
     6121 ttaagtacct ggcgggggaa cgtccagctc tcctctctca gctcacttcg atcgtgcgag
     6181 acaaagtgcc ggatatagaa gaggtggaga tggaatttgt tctggagcag gcattgttga
     6241 accttcagtc gcgtctgtga ttctctccgg cgatttcttt tgttgttgtc attgaatcag
     6301 aaattgcccg ggatcgccca tgggagagac gaatgcgcag tttcgccaga tacttgatgt
     6361 ttctcctatt cccttcgctc tgaatgatga aaatcaaaac ataacatatt tgaaccgggc
     6421 ttttacacgc acgtttggtt atgatttaac ggacatccct actctcgcag aatggtggcc
     6481 tcgcgcttac ccggagccgg cttatcgcga acgggtgatg aagtcctggc aggagagaat
     6541 ggagaaagcg accagagaag gcacgcaatt tgaagccctg gaattgacga ttcgctgcaa
     6601 agatggttca gatcggattg taatcgtgag cgcggtttct cctggagatg cgcctgaagg
     6661 tgtgtatgcg gtcatgctct acgacatcac cgaaagaatg aacctgctgg ccgcagccca
     6721 gcgcgcggat cgcctcaatt cacttggtgt tcttgcaggc ggaatcgccc acgattttaa
     6781 taatctcctt tcttctattt ttggatacat ggagctcgcg cggggtgcca ctacagagag
     6841 ccgcgttatt gaatatctcg atcgcggtct gtccgcgttt tcgagagcgc gaggactcgc
     6901 tcaacagctt ctcacattcg cacgcggcgg agatcctgtc atgcaatcag gcaatctcgg
     6961 gacactcctt tcggaaacgt gcgaatttgt tctggccgga tccaatgttc actatcagtg
     7021 ccacgtagca gacgatttgt ggccttgcaa tttcgatacc ggtcagatga accaggtttt
     7081 ttctaacctg ttgatcaatg cacgtcaggc aatgccggac ggaggaaaca taggggcagt
     7141 cgcttcgaat cgttctctga atgaaacttc tgttcacccg gcagggccat acgtcgagat
     7201 tcagatttca gacagcggac gcggaattcc agcggagcaa atttcgcaca tctttgatcc
     7261 attctatagc actcgcccag gaggtacagg gctgggcctt gccacttgct tttccatcat
     7321 tcagaaacat gcaggattca tcgatgtaga atccactccc ggcaaaggag caacgtttcg
     7381 tattttgatt cctgttgcaa agtcagggaa ttcgcgcgga gcaagtttcg ccaaacacag
     7441 cgcaggtgaa caaagacttc tggttatgga tgacgaagaa ctcattcgtg cagtgtctca
     7501 ggatctcttt gcctctgcag gattttccgt tcaggtagcg gctcacggtg aagaagcact
     7561 cattttgtgc gatgaagcgg ccaggagcgg cagacctttt catgctgcga ttctggattt
     7621 gacggttcct ggacacatgg gtggaaagga tcttgccaga ctactcagga aggatcaccc
     7681 ggaaattgca ttgtttgcgt ccagtggcta ttcagaagat ccagtaatga cggcccccgc
     7741 agaatacgga ttcgtcgcca gcattcgaaa gccatatctg attgcggatg cagcgcagct
     7801 ggttcgccag gttcttcgca cttagcgttg gcttgacgaa gagcggcagt ggtgcaaata
     7861 acgatctaaa ggatcgtaga ttcatgaagc tttctaaaaa aacagactat gcgctgcgtg
     7921 ccatctttac tctcgtagag cagcatgcga ccggggaacc ggtttccatt tccgatctag
     7981 cgcaagccaa tcgcatacca aagagatttc tcgagcacat catgctggat atgaaatcgc
     8041 aaggatgggt gtcgagcact gcagggaagt acggaggata tcgactcgca aaacatccat
     8101 cgcagatcac gatgggagaa atcgttcgtt attttgacgg aattctcgcg cccattggtt
     8161 gcgtttccat cgctcgttat gaaccatgca agcaggaacg cgtgtgtcgc tttcgtcgtc
     8221 ttctgctgaa tgtgcgaaac gccgtcgcag gaatgatgga tcgtgcgacg cttgaatctg
     8281 tgttcgcaga aggaatcgtc acgcagaaag aagtgatgca cgtgtctttg atgggcggag
     8341 atggaatctg actcggaact tgagcatgct gattcgtcat atcgcagagc aatggtaagg
     8401 cgttaaacat ttgacctgaa gcggtctgcg aaaattctat cttcgcatgc ggaaacgatt
     8461 tttgacaacg tcatctgcaa cgttcgcccg cagttggttc gcagccatgg aactattggt
     8521 tatcgtggga ttctctgcgt cctgta
//
LOCUS       DAORFF010000090         8520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4011_length_8520_cov_3.161370, whole genome shotgun sequence.
ACCESSION   DAORFF010000090 DAORFF010000000
VERSION     DAORFF010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4011_length_8520_cov_3.161370"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /gene="sppA"
                     /locus_tag="PKK76_05410"
     CDS             <1..809
                     /gene="sppA"
                     /locus_tag="PKK76_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNL67939.1"
                     /translation="VRGVIEDGSGGPGGQGADRIVERIRSAQEDPAVRGMLLSINSPG
                     GTTGATKKIYDKLIEFRKEKPIVCYVTDIAASGGYYLCSAANKIYAYRGSIIGSIGVI
                     MIHPDAEELLRKVGLTVSVIKAGRFKDMSYPFRTMTEEERLMNQKLVDDAYEQFVRDV
                     AEGRRQPETNVRKWAEGKIYSGTSAKAEQIIDEFGGETEALLSMKTEFIKTKDDLPLL
                     RPRKEFFEEFLTGLSGRSYDLSSGRVYYLYPDSTTVARMILGPDLVKIAK"
     gene            815..1384
                     /locus_tag="PKK76_05415"
     CDS             815..1384
                     /locus_tag="PKK76_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67940.1"
                     /translation="MSKFVELAAAILQKPEDSVRFANNPGPFSSIIPAFSAFSLAAAG
                     YLLRDYYEGIFLFTISFHASVLTLVYVLFASLTSAVLDARVQTQSGRSPRASQARRVL
                     LQSLLPLIFAFPAATIAHITPVPGFVFVPLLLLLYAWAFITALRSLQYLYELSIRDIA
                     RQLVISSVITLAFPAALVVLLFVRLAAFL"
     gene            1393..2127
                     /gene="surE"
                     /locus_tag="PKK76_05420"
     CDS             1393..2127
                     /gene="surE"
                     /locus_tag="PKK76_05420"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNL67941.1"
                     /translation="MRILLTNDDGLFTNGMLHLEEALRDGAEVWCIAPDRERSATSQA
                     LSLRETLLLKKISDRRYTVNGYPTDCVNIALFSGEFPRFDLVISGINHGVNLGDDIHY
                     SGTVGAARHATLHGHPAIAMSCPIRGHDGDFRRPAAWLAQWIKSNLKALQPGKLWNIN
                     YPEESAGANGFPEVVITRQGRRIYQDRYELLSEGEGSRIYKLLETTMGRKMEPGTDFD
                     AFESGRISLTPITTDTTDQERLYGAL"
     gene            2124..2771
                     /locus_tag="PKK76_05425"
     CDS             2124..2771
                     /locus_tag="PKK76_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67942.1"
                     /translation="MKSEKNEKQVLERILTIKQMIQEGRGGPLELHELGICYYLIENF
                     RSASKFLAELAGKYPDYVEIASVQSLRCYCLVQEGELLEAELLLKERIPRDPSNTTLL
                     GLLAHVQEKTHRIQEAIATHKRIIGIDPGNINSRNSIGCLLAEHGTPADYPEAMEHLR
                     IAVSNKPEHPAYLDSLGILLARQGDRDRARRAFVKALSRSPANTVILDHLKDMIG"
     gene            complement(2795..3721)
                     /gene="pyrB"
                     /locus_tag="PKK76_05430"
     CDS             complement(2795..3721)
                     /gene="pyrB"
                     /locus_tag="PKK76_05430"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNL67943.1"
                     /translation="MFSHLHILDADQFSREDLETVLSSTDRIAQLLKENKTFGLLDGK
                     ILASLFFESSTRTRLSFETAMNRLGGRVVTTVGFQFSSISKGETLYDTMKMIEAYADI
                     AVIRHPVEGSSRIAAAAVNIPVINAGDGIGQHPTQALLDLYTIFKEKGAIEGLRIGFV
                     GDLKFGRTIHSLIKMLRHYNVEIVLISPVDLALPESYRKELRRENVKFEETEDLKALW
                     DCDVGYITRIQEERFADRSEFERLKDSYNVNRAFLLASRKPTTILHPLPRVAELSTDV
                     DDMPTAAYFRQAGYGVVIRMALLMLCLGQPIP"
     gene            complement(3782..4561)
                     /locus_tag="PKK76_05435"
     CDS             complement(3782..4561)
                     /locus_tag="PKK76_05435"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HNL67944.1"
                     /translation="MVKYTLLLLLLPFHLMAAMPPGVAPGATFDSRGHVWVLTANNQQ
                     VIWFENGVKKAEGPFEHNQKRGVWTHYYESGKIKGTGAYEANLKQGPWKIYHPEGKVE
                     SEGSFKNDARSGKWVYYDKMGNKSAEGEFRAGMKEGPWVSYYGNGQVASRGSYRAGEA
                     QGAWEYFFEKGQPFQSGQFDGDVRIGTWTICVAPQGPCGKEVYNSASKSGAPAVSGLN
                     PAELTSQEIRNAKGNPARVLESMEGDVPDAVPSSINQKWND"
     gene            complement(4589..5827)
                     /locus_tag="PKK76_05440"
     CDS             complement(4589..5827)
                     /locus_tag="PKK76_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HNL67945.1"
                     /translation="MRHKQALSETSERRWNIVLDELRREIPPPLFRAFIAPVSVSCEN
                     EDLHIIAPDENKRRHLDKHYLTLLESAWRKQASTGRIRLLTLEKEREQLQERAQPIPV
                     SSHFDFLIHERVSPLVSEMLNLNFRAPLYFLHGETSTGKTELMRQIETRAKAAGQTVR
                     RTGLDGFIVEFAAACKRKSTVDFRKSWKDCRVVMIDDLQFLKPGAKQTREELRHLAEH
                     SLAGNLTLILAADVPLRQIHMEEDLRSRLAAGAHIQLPPPDRSMRIRLFERFCKEAGV
                     TPQESLSARAARSYKDPRALRSLALQLSVDPAAGDRIPAHEPEFSSILRHTAAFCGLP
                     ESEIMGDGQSRRTVGARRMAMVLVAELMNRTPTQIARLFNRSGHTSVLYAMARMKKDL
                     EEDLFIANQLAELRRLILAD"
     gene            complement(5908..6873)
                     /gene="trxB"
                     /locus_tag="PKK76_05445"
     CDS             complement(5908..6873)
                     /gene="trxB"
                     /locus_tag="PKK76_05445"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNL67946.1"
                     /translation="MSAIHSVVIIGSGPAGHTAAIYSARAGLSPAMYEGFMAGGVAAG
                     GQLTTTTEVENFPGFPEGITGPELMDRMRTQSQKYGTTIFTETIERVDFSQRPFKIYS
                     QNDPDTAILAHTVIIATGATAKTLPIGGFQDYWQKGISACAVCDGSLPIFKNVPLFVV
                     GGGDSACEEATYLSKFGSRTYLVHRRDKLRASQIMQERVLKNPRIEILWNSEIVEAAG
                     DGRKIGSVVVRNIASGETRKQDAGGLFFAIGHKPNTDVFKNILSMDEDGYLITKPNST
                     ATNIEGVFAAGDVQDKKYRQAITAAGSGCMAALEAERFLAATGAH"
     gene            6953..7684
                     /locus_tag="PKK76_05450"
     CDS             6953..7684
                     /locus_tag="PKK76_05450"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNL67947.1"
                     /translation="MAKKAKKSPSKKSAKKSAPSKNKKSAKKAVKKSIKKSTKKSVKK
                     SVKKVIKKAPARKAPAKVAPAKKAPAVSAPSRTTAVPTPVAPQAPAVSYPDQIADRSV
                     RLTDGSTSSLSGLSGPKGLVLYFYPKDDTPGCTVEACNFRDAESKIRAMGYKIAGVSP
                     DTSTAHQKFTAKYHLNFPLIADEDHSLCEAAGVWQEKSMYGKSYMGVARTTLVLDPSL
                     NVKKVYEKVKPDGHADAIAGDLSGL"
     gene            7681..>8520
                     /locus_tag="PKK76_05455"
     CDS             7681..>8520
                     /locus_tag="PKK76_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67948.1"
                     /translation="MKTQKKTLPFEITEPAFEIKVGKAPAGAILVMPMFKDQAVKTLP
                     LSGEGKSLLEEFVKSKTFQGKSSEICDLSARGLIVFGLGAKEAFHPDTIAGQMIHIAP
                     KLLKYEKSTYVLRFTEEVWQALKGFRDGKVEPAEDDESFSDYFAPMTDEELLSQCVSS
                     LLAGSDSMEMLKSKKKSAEKKTVVYVEAEYLKKKLADAVARGVAVGELSHSMRYVESL
                     PGNFFHPEAFQTYTEQLTRKHGVSLKVFGISDLEKMGCGGIVSVGKGSRIPPRMMILE
                     YKPK"
BASE COUNT         2076 a         2103 c         2187 g         2154 t
ORIGIN      
        1 aagttcgcgg tgttatcgag gacggttcag gcggaccggg tggtcaggga gcagatcgca
       61 tcgtagagcg aattcgctct gcacaggaag accccgctgt tcgcggaatg ctactatcca
      121 tcaacagtcc aggtggaacc acaggcgcca caaagaagat ctacgacaaa ctcattgaat
      181 ttagaaaaga aaagccaatc gtctgctatg tgactgacat agcggccagc ggcggctatt
      241 acctttgctc cgctgccaac aagatctatg cgtatcgcgg atctatcatc ggatcgattg
      301 gcgtgatcat gattcacccg gatgcagaag aactcctgcg caaagtcggc ctcacagtca
      361 gcgtcatcaa agcgggcaga ttcaaagata tgtcctatcc atttcgcacg atgacagaag
      421 aagaaagact catgaaccag aaactcgtgg acgatgcata cgaacaattt gtgcgcgatg
      481 tagccgaggg tcgtcgtcaa ccggagacca atgtgcgaaa gtgggcagag ggaaaaatat
      541 attccggaac gtctgcgaag gcggagcaga tcattgatga atttggcgga gaaacggaag
      601 cacttttgtc tatgaaaacg gagtttatca aaacaaaaga cgatcttcct ttgcttcgcc
      661 cgagaaaaga attcttcgaa gagtttctta ctgggctgtc cggtcgttct tacgatttga
      721 gcagtggccg tgtatactat ctgtatcctg attcaacgac tgtggccagg atgattctgg
      781 ggccggacct ggttaagatt gcaaaatgaa ttggttgagt aaatttgtag agcttgcggc
      841 ggccatcctt cagaaaccgg aggactctgt tcgtttcgca aacaatccag gaccgttcag
      901 tagcatcata cctgctttct ctgctttttc gctggcagcc gccggttatt tgctgcgtga
      961 ctactacgaa ggcatttttc ttttcacgat ttcctttcac gcctctgtcc tcactctggt
     1021 ctacgttttg tttgcctctc tcacgtctgc cgtactcgat gcgcgtgtgc agacacagtc
     1081 tgggaggtca ccgcgagcaa gtcaggccag aagagttctg ttacagtctc ttctaccgct
     1141 gatcttcgca tttccggcgg ctaccatcgc gcatataaca cctgtgcccg gtttcgtatt
     1201 tgttcctctt ttgttgttgt tgtacgcctg ggctttcatc actgcacttc gctctcttca
     1261 gtatctttat gagctttcca ttcgtgacat agcaaggcag ctcgtgattt cttccgtcat
     1321 cactctggcg ttcccggccg ccctggtcgt ccttcttttc gttcgcctgg ccgcattcct
     1381 ctagttcagt agatgagaat tctccttaca aatgatgatg gtctctttac aaacgggatg
     1441 ctgcatctgg aagaggcatt gcgcgatggg gcggaagtgt ggtgcatagc accggaccgc
     1501 gaacgaagcg ctacttccca ggcactcagc ctgagagaaa ctcttctctt gaaaaagatc
     1561 tcagatagga gatacacagt aaacggctat ccaaccgatt gcgtaaacat tgcacttttt
     1621 tcgggagagt ttccgcgttt tgatcttgtg atctctggaa tcaatcacgg cgtaaacctc
     1681 ggggatgaca ttcactacag tggaaccgta ggagccgcgc gtcatgcgac cttacatggt
     1741 catccagcca tcgctatgag ttgtccgatc cgcggtcatg acggagattt cagaaggccg
     1801 gccgcatggc ttgctcagtg gatcaaaagc aatctcaaag cactgcaacc tggaaagttg
     1861 tggaatatca actatccgga agaaagtgcc ggtgccaacg gttttccgga agtggtcatc
     1921 actcgccagg gcagacgcat ctaccaggat cgatacgagc ttctgtctga aggagaaggc
     1981 tcacggattt ataaattact cgagactacg atgggtcgca agatggaacc cggaactgac
     2041 tttgatgcat ttgaatcagg caggatttct ctgactccca tcacaacaga cactacggat
     2101 caggaaaggc tatacggagc gctatgaaat cggaaaagaa cgaaaagcaa gtgctggaga
     2161 gaatcctgac catcaagcag atgattcagg aaggaagggg cgggcccctc gagctccacg
     2221 aactgggtat ctgttactat ttaatagaga atttcagatc cgcttctaag ttcctggcgg
     2281 agcttgcagg caagtatcca gactacgttg agattgcttc cgtccagagt ttgcggtgct
     2341 actgcctggt tcaggagggg gagctactcg aggcggagct tttgctcaaa gagaggattc
     2401 cgcgcgatcc ctccaatacc actcttctgg gtttgcttgc tcatgttcag gagaagacgc
     2461 accgcattca ggaagcaatc gccactcata agcgaatcat tggaatcgat cctggaaata
     2521 tcaacagtcg caattccatt ggatgcttgc ttgcagaaca tggcactccg gcggattacc
     2581 ccgaggcaat ggagcacctt cgcatcgcag tgagcaacaa accggagcat cctgcttatc
     2641 tggattctct gggcatcttg cttgctcgac agggagaccg tgacagggcg cggcgtgcgt
     2701 ttgtaaaggc gctcagcaga tctccagcaa acactgtgat cctcgatcat ctcaaagaca
     2761 tgattggttg attttcttta gtaagtttga cgattcaagg aatgggctgt ccgagacaga
     2821 gcatgaggag tgccatgcga atcacaactc cgtagcctgc ctgacgaaag taagccgctg
     2881 ttggcatatc gtctacgtct gttgagagtt ctgccacgcg aggaagtggg tggaggattg
     2941 tggttggttt tctagaagca agaaggaagg cgcgattgac attgtaggag tctttgagcc
     3001 gttcaaattc gcttctgtcc gcgaagcgtt cttcctggat gcgcgtgatg tatccaacat
     3061 cgcaatccca gagagctttg aggtcttctg tttcttcaaa ctttacattt tcgcgtcgca
     3121 gttctttgcg ataggattct ggcaatgcga ggtccacagg cgaaatcaag acaatctcta
     3181 cattgtagtg gcgcagcatc ttgatcaggc tgtgaatggt tcttccaaat ttcaaatcac
     3241 ccacaaatcc aattcgcagt ccttcaattg cacctttttc tttgaagatg gtatacaaat
     3301 cgagaagagc ctgtgttgga tgctgtccga ttccgtctcc ggcattgatt acggggatat
     3361 ttaccgcggc cgcggcgatg cgactggatc cttccactgg atggcgaata acggcaatgt
     3421 ccgcatacgc ttcgatcatt ttcattgtgt cgtagagagt ttctcctttg ctgatggagg
     3481 agaattgaaa accaacggtg gtaactacgc ggccacccag acgattcatc gctgtttcaa
     3541 aactgagtct ggttctcgtg gaagattcga aaaataaaga tgcgaggatt ttgccgtcaa
     3601 gcaggccgaa cgttttgttt tctttgagca gctgggcaat tctgtcagtg gaactcagta
     3661 ctgtctctag atcctcgcgc gaaaactgat ctgcgtcgag gatgtgcagg tggctgaaca
     3721 cgagtccact gagccattca ggctcacgaa gaaaagtgaa aaatcacctg ctagaaaaaa
     3781 gctagtcgtt ccacttctgg ttgatcgaag agggcactgc gtctggcaca tctccttcca
     3841 tggattccag aacgcgggca ggattcccct ttgcatttct gatttcctgc gaagtgagtt
     3901 ctgcggggtt caggccggat actgcaggcg cgcctgactt agacgctgag ttgtacactt
     3961 cctttccgca aggaccttga ggagcgacac agatggtcca ggttccaatg cgcacgtctc
     4021 catcgaattg gccactctgg aatggctgtc ccttttcaaa aaaatattcc catgctccct
     4081 gagcttcacc cgcgcgatag ctccctcggc tggccacctg gccatttcca tagtaagaaa
     4141 cccacggacc ttctttcatt cctgcgcgaa attcgccttc cgcgcttttg tttcccatct
     4201 tgtcgtagta gacccactta ccgctgcgcg catcgttttt gaaggatcct tctgattcca
     4261 ctttcccttc cgggtggtag atcttccacg ggccctgttt caaattggct tcgtaagcgc
     4321 ctgtcccttt gatcttgccc gattcataat aatgagtcca gactccgcgc ttctgattgt
     4381 gctcaaaagg cccctctgcc ttcttcacac cgttctcaaa ccagatcact tgctggttgt
     4441 ttgcggtgag gacccacaca tgtccgcggg aatcaaatgt ggcccccgga gccacgccag
     4501 gaggcatggc ggccatgagg tggaaaggca aaagtaagag caaaagggtg tatttcacca
     4561 tatgatgatc ggtaattgca gaaaaaactc aatctgcaag tataagacgt ctgagctctg
     4621 ccagctggtt cgcaataaat aaatcttctt ccaggtcttt cttcattctg gccatcgcgt
     4681 acaggacgct cgtatggccg ctgcgattga atagcctcgc gatctgtgta ggagtgcgat
     4741 tcatcaattc tgcgacaaga accatggcca ttctcctggc ccccacagtc cgtctgcttt
     4801 gcccgtctcc catgatttcg gattcaggca agccgcagaa ggcggccgta tgtctcaaaa
     4861 tggaggaaaa ctccggttcg tgtgccggga ttcgatcccc tgccgccggg tccacgctga
     4921 gctggagggc caggcttcga agggctcttg gatctttgta ggatctggcc gctctggcgg
     4981 agaggctctc ttgcggcgtc actcctgcct ctttgcagaa tcgttcaaaa agcctgattc
     5041 gcatggagcg atccggggga ggaagttgaa tgtgagcgcc cgctgccagg cgagagcgaa
     5101 gatcttcttc catatggatt tgcctgagcg ggacatccgc tgcaagaatc agtgtgagat
     5161 ttcctgcgag agaatgctct gccaggtgcc tgagttcttc gcgtgtctgt tttgcgcctg
     5221 gtttcaggaa ttgcagatca tcaatcatga cgacacggca atctttccag gattttctga
     5281 agtctactgt ggacttgcgt ttgcacgctg ctgcaaattc aacgatgaat ccatccaggc
     5341 ctgttcgccg gacagtctga cccgctgcct tcgcacgagt ttcaatctgt cgcatgagct
     5401 cagttttgcc cgtcgatgtt tctccgtgca gaaagtaaag cggagctctg aaattcaagt
     5461 tcagcatttc tgaaacaaga ggggaaacac gttcgtgaat caagaaatca aagtgagagg
     5521 aaacggggat gggctgcgct ctttcctgaa gttgctctct ctctttttcc agagtgagaa
     5581 gtctgattct gcctgtggac gcctgctttc tccacgcgct ttccagtaga gtcaggtagt
     5641 gtttgtccag gtgccgtctc ttattttcat ccggcgcaat gatatgaaga tcttcatttt
     5701 cgcaggaaac cgaaacgggt gcaatgaacg cacgaaagag tggaggagga atttcccttc
     5761 tcagttcatc tagaacgatg ttccatctgc gttccgatgt ctccgataat gcttgtttat
     5821 gtctcatccg tgattatgtc gcataagaaa aagtgcgatc ggacattgtc gcctgattct
     5881 tccagcgacg caagctaaat caagtgatca gtgcgcgccg gtggctgcga gaaatctttc
     5941 tgcttccaga gcggccatac agccacttcc tgcagctgtg attgcctgcc tgtatttctt
     6001 atcctgaaca tcaccggccg cgaaaacacc ttctatgttg gttgcagtgc tgtttggttt
     6061 tgtgatcagg tatccatctt cgtccatgct gaggatattc ttgaaaacgt ccgtatttgg
     6121 tttgtggccg attgcaaaaa acaaaccgcc tgcatcttgc ttgcgtgttt ctccgcttgc
     6181 aatgttgcgc actaccacgc ttccgatttt tcttccgtct cctgccgctt ctacaatttc
     6241 tgaattccac agaatctcta tgcgcgggtt tttcaaaact ctttcctgca tgatttggga
     6301 tgcgcgaagt ttgtctcttc tatggaccag gtacgtgcgc gagccgaatt ttgaaagata
     6361 ggtggcttct tcgcatgcag aatctccacc acctacaacg aagagaggaa cgttcttaaa
     6421 aataggcagt gagccatcgc atacggcaca tgctgaaatt cccttttgcc agtaatcctg
     6481 gaagccaccg atcggaagag ttttcgcagt ggcacccgtg gcaatgatga ctgtatgggc
     6541 gagtatggca gtgtctggat cgttctggct atagatcttg aaaggtctct ggctgaaatc
     6601 tactctttct atggtttctg tgaagatagt agtgccgtat ttctgagatt gagttcgcat
     6661 tctatccatg agttccgggc ctgtgattcc ttccgggaag cctggaaagt tctccacctc
     6721 tgtagttgtg gtcagctgac cgcctgcggc aactccacct gccatgaatc cttcatacat
     6781 ggcgggagac agcccggccc gggcagagta gatggccgcc gtgtgtccgg caggtccgct
     6841 tccaatgatg acaactgagt gaatcgcgct catgcagtca gaaaacaaaa accgtctgcc
     6901 gatcaaataa aaattttcag gactcgtgcg gtgcgatttt gtagtaagtg ctatggcaaa
     6961 gaaagcgaag aaatccccct ccaagaaaag cgcgaaaaag tccgccccga gcaaaaacaa
     7021 gaaatcggca aaaaaggccg ttaaaaaatc gattaagaag tctaccaaaa agtcggttaa
     7081 gaaatcggta aagaaagtca tcaagaaagc acctgccaga aaggctccag cgaaggtagc
     7141 accggcaaag aaagcgccag ccgtaagtgc accttccagg acaacagccg ttcccactcc
     7201 agtagctcca caggcacctg cagtcagcta cccggatcaa atcgcagaca gatctgtgcg
     7261 acttacagac ggaagcacat cgagcctttc gggtttgtcc ggtccaaaag gactcgttct
     7321 ctatttctat cccaaggatg acactccagg gtgcacggta gaagcgtgta acttccgtga
     7381 tgcggaatca aagatccgcg ccatgggtta caagattgcg ggagtctctc cggatacttc
     7441 cacagctcac cagaagttta cagcaaagta ccatttgaat tttccgctca tcgcagacga
     7501 agatcattcg ctgtgcgaag cagccggagt ctggcaggaa aagagcatgt acggaaaatc
     7561 gtatatgggc gtggcgcgaa ctacgttggt actggatccg tcgttgaatg ttaagaaagt
     7621 ttatgaaaaa gtgaaaccgg acggacatgc agatgcgatc gctggagatc tctcaggcct
     7681 gtgaaaacac aaaagaaaac attgcccttt gaaatcaccg agcctgcctt tgaaatcaag
     7741 gtgggaaaag ctcctgccgg cgccattcta gtaatgccca tgttcaaaga ccaggcagta
     7801 aagactttgc cactgtcggg agaaggaaag tctctcctgg aagagttcgt aaaatccaaa
     7861 acgtttcagg gaaagtcctc tgaaatttgc gatttgtctg cgcgcgggct cattgtattt
     7921 ggtctgggag cgaaagaagc atttcacccg gatacaatcg cagggcagat gattcacatt
     7981 gccccgaagc ttctgaagta cgagaaaagc acctacgttc tcaggttcac ggaagaggtc
     8041 tggcaggcgc tgaagggctt cagggacggg aaggtggagc ccgcagaaga cgatgaatca
     8101 ttttcggatt attttgcgcc catgaccgac gaagaacttc tctcgcagtg cgtctcgtca
     8161 ttgcttgcag gttccgattc gatggaaatg ctcaagtcaa agaagaaatc ggctgaaaaa
     8221 aagaccgtag tgtatgtgga agcagagtat ttgaaaaaga aactcgcgga tgcggtggcg
     8281 cgtggtgtcg cagttggaga gctttcgcat agcatgcgtt atgtggaatc cttgcctggg
     8341 aacttctttc atccggaggc atttcagacg tatacggagc agctcactcg caaacatgga
     8401 gttagcctga aagtattcgg gatatcagac ctggaaaaga tgggatgcgg cgggattgtt
     8461 tccgtcggca aaggatccag aattccacca cgaatgatga ttctagagta caaaccaaag
//
LOCUS       DAORFF010000091         8511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4018_length_8511_cov_3.623463, whole genome shotgun sequence.
ACCESSION   DAORFF010000091 DAORFF010000000
VERSION     DAORFF010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4018_length_8511_cov_3.623463"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..941)
                     /locus_tag="PKK76_05460"
                     /pseudo
     CDS             complement(126..941)
                     /locus_tag="PKK76_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016559276.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1173..1907
                     /locus_tag="PKK76_05465"
     CDS             1173..1907
                     /locus_tag="PKK76_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67949.1"
                     /translation="MRRIFQIIAAVTLACPLHAESSENEMQSYFLSGAAAASGILEGG
                     VIAPAWHAYIRDVPRIDRVQSLILFSSLDRPGAALTTEIFRQESQTDYRHAANLRLQA
                     YGGTLALFGLAGVLATKPVPSWDHPAVPVLGGVSAGMSLRWLVQWNRYSNVMRQLSTN
                     QRTTQIALLAAAPSDRLTLVSYYLRQTEKKTFARTRRLESACASVDFALGAAAFFYAY
                     ASTNPAPSTSAFFFAGPDRAGFQIRF"
     gene            2034..2663
                     /locus_tag="PKK76_05470"
     CDS             2034..2663
                     /locus_tag="PKK76_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67950.1"
                     /translation="MNLPLTSISPPRLRRISLLTGVSGIYALSHSWISRLALLGLPIT
                     VMDCADRFDPFAIAEEAASQDVKPDVLLETITCQKIATPLQFLGAARGILNARRGGVA
                     VILSPLRHFLDIGESAHGRELLLDGFLSVLSELSVRSRPVLLVEAQQDAPLFHSAFDR
                     MAALASPIWRILPDSVEVAENKKTQTTRSVDRMAQNNLEGISPDRLATG"
     gene            complement(2660..3916)
                     /locus_tag="PKK76_05475"
     CDS             complement(2660..3916)
                     /locus_tag="PKK76_05475"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNL67951.1"
                     /translation="MSNRLIYLLDADARARGDLAEQIGHYGYGVSTFASLETLREAVA
                     QSVPAVIVAAMDRLESEMHISEIQRNLNPSPCVVFLGGSDDMQMRLRSVRFGGSGFFS
                     KPVNVDALVTLLDRLTSPFVVQAYRILIVESDGELSQHLARILGAAGMETHVVSNPAE
                     MLRAGDLFHPELILMNLYYPEILGMELATVLRQDPRYERIPIVFLSSELDPARQMAAL
                     AKGGDDCLTLPIHPDYLALIVASRVEATRSIKSLYFRDGLTGLLNHRAMLDSLEMKLE
                     LAGARGVELPFVMVDIDRFKLINDTYGHPVGDTVLKSLGRLLKQRVWSKDLVGRLGGE
                     EFGVIVNSMDAATAARVFNEIRIDFAQIRHTSSEGDFYATFSCGVAWFPRYATSAEIE
                     SAAERAVRQVKALGGNRVGLIGRHES"
     gene            complement(3965..4414)
                     /locus_tag="PKK76_05480"
     CDS             complement(3965..4414)
                     /locus_tag="PKK76_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNL67952.1"
                     /translation="MKRKSLDQSEYKRAALSCFNFSFRNAARAVTLLYDRALAPAGIR
                     STQYSLLIVLAGYGSLTINDLASGLGMDRTTLSKNLRPMVHRKLIQFESQKDRRKKAL
                     VLTKAGYTMLEKCTPLWEEAQDSVQKMLGEDARDVQRRLVRVARIRN"
     gene            complement(4395..4922)
                     /locus_tag="PKK76_05485"
     CDS             complement(4395..4922)
                     /locus_tag="PKK76_05485"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNL67953.1"
                     /translation="MELQAITPRSTFRVSFHDCDAFGHLNNARYISHFIDARTDHLRD
                     FHGYDVYADAEKTGKNWVVQNTSIRYLYPAKLNQLVEIETRLLRFEKHRLFPEGIMRD
                     PETGKVHAIVWFDLAYVDLKTGRPARHGGALTELVSRIVETDPDMERFDFAKRVAQIM
                     SIHGRRETSREAEVA"
     gene            complement(5016..6776)
                     /locus_tag="PKK76_05490"
     CDS             complement(5016..6776)
                     /locus_tag="PKK76_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNL67954.1"
                     /translation="MIANNYFLDDPDLQTFFNHLIDWDSIVQAVEGNDFADHRRFLET
                     GNSRFEMAPASVEEAVGLYRSSLESLGEFCGKELSQVSQAMDRAALQFQNGRVVFPSE
                     TISVYEKFRETGLMPYSISRHAGGLGLPATVGAFIVMLLARADVSFCMTITLLNLAQI
                     VARYGTEEQIEQFAAKAAAGETLFAMALTEPDYGSDLNSVRMTAIRSEDGSYRLNGTK
                     RFISQGCGLGPYPCTLLTLARTGKVGGRARGLSVFLVKSEDVTIAGIESKMGIHASPT
                     CEVVYEDAPGQILGEEGLGLTRYTAGMTNFMRLGSAAGGPGGGAGAYFECEKYASSRI
                     QFGKPIAQIPAVAEMIQKIRREVNAMRLLAFETARIVDLFQHHQIRLEKEGVEDRDIR
                     RDETIKTWSTLASILTPVAKYYCSEEGHRCGNLAVQIHGGAGYTEDYDVSRMFRDSRI
                     NTIYEGTSQIHVRIATGAISAGMGEGANFRKYLEDLYSQVRAPSTFLIEQFRLLEESV
                     DCFRMIESEDSRERLAENLMIVAARFLCTVLYEKALGLLANHSDLERWRADCRSYGLE
                     SAAIASGCFYRLKNSEFAAV"
     gene            complement(6826..7842)
                     /locus_tag="PKK76_05495"
     CDS             complement(6826..7842)
                     /locus_tag="PKK76_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67955.1"
                     /translation="MNLQGRVVTLAGSGFLGGDVEGRGSSAKFSGLGAWAANGSFAFG
                     GDIGNKKIKKIDLRSADVAIIAGPAAGAVVSGDADGSSAVSTFNDPQMTTLSGNALYV
                     VDGTNNKIRRIDLATGIVSTFAGPSSGTTTFGDTDGVGNAARFFAPTGITTDGTNLYV
                     VENGNCKIRRVSLATQQVTTIVGPLPGSNGCSLDNDGIGNAARLFAPGAITTDGKNLF
                     VSDNSKIRKVDLNTLQVTTIAGPAPGSSGTGHVDAAGTAARFGSILGLATDGFSLFVS
                     ESYYVRVLNPGTQVVNTLAGDGNLGLTDGVGPGAQFTTQLRLSASDGAHLILGDGNNV
                     RLLE"
     gene            8155..>8511
                     /locus_tag="PKK76_05500"
     CDS             8155..>8511
                     /locus_tag="PKK76_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67956.1"
                     /translation="MNKASVKAVCLFVFVAIPGCGRSAGEPAVVQGQMDLSNHELLDG
                     QRIKLDGAWEFYHGQFLVSGQFPQRTLDFLSLPGYWNERGATVDEYPGHGFATYRAHL
                     RLHPRDVKRRLALYVPH"
BASE COUNT         2060 a         2399 c         2042 g         2010 t
ORIGIN      
        1 gccggcgcaa ccatggtaga aactgcgtcg caagaaacac ttctgcgaag ccttggatgc
       61 gacactgccc agggatttca cttcgcagaa ccggcagact cttctgcgtg gctcagccag
      121 gcgcgctagc gcgtgtaacc aaaaaagact gctttctcca cgaaaccgaa attctgccgg
      181 gcgcgatcct tgcttctacc atttgttaga atgtctgaaa gatcggcgta gcctcccttc
      241 cctgagagct ctacgaagaa agatcgaaag aaatcaaaac gcaacccgac catcgcagaa
      301 acgccgtatc ccgaaagatg atacacattg tttctgcctt cgccaaaaag tctaacgtcg
      361 ctacgacaaa ctataggacc gccaccaatc gaagagagca tgctcaagcc atgctctcca
      421 ctgtccgaca tccagatttg ctgaatgaat ccaacatcca cctgaaaata gttcagtcca
      481 tccgtatgat caaatttcaa aaagtccgga gtgatgttga ttggctctcc cagatgcact
      541 ccttcgtatt gcttgtactg cgcgggaaat agatagagat aggggagaga ttcaagagaa
      601 ggctgcagat gcgcgcgctg aatcgcaaga ggatctatat acccgtagat attggaagcc
      661 tgtccgcgcg tgagaacgta cttcatgtga tcctgaccaa atgaaagagt aaagtgattg
      721 gtgatgtatc gcgtgtagcg aaagctaaac tgaggcacgt caaattcgct tggactgaga
      781 tagactggcc cgatgttctc tggtttgtct tttgccacaa cgtcttttaa agtgaaacga
      841 taccccggtc cgcgaaagtt gatatcactc tgagtgtatg cgtcgcggtt gtaaccccac
      901 tgaatactcc atgttccttt tctctcatca atcgagcgcg gtccgggtgg aagtggagag
      961 atgaccgggt ccagagtcaa tggcatcaga ggagtttcct gagcccaaag agtgccgcac
     1021 acaaccagtg acaaaacaga gctaaagagg attgctttca agaggccagg attccaatgg
     1081 attgttgata aggaaatcgt tttcacattc aatcgttccc agtttttctc tgattcactt
     1141 gccatatcga ggattctgcc caaaatttgg tcgtgcgtcg tatttttcag atcatcgctg
     1201 ccgttactct tgcctgcccg cttcatgctg agtcgagtga gaatgagatg cagagttact
     1261 tcctttcagg cgcagctgca gcttctggaa ttctcgaagg tggcgtgatt gccccggcat
     1321 ggcacgcata tatccgcgat gttccccgca tcgaccgcgt gcagtccctc atcttgttta
     1381 gttctctcga tcgccctggc gccgcgctca ctaccgaaat tttcagacag gagtctcaga
     1441 cggactacag acatgcagcc aacctgcgcc tccaggcata cggcggaact ctggctctct
     1501 ttggtctggc cggagtgctt gccacgaagc ctgtgccgtc ctgggatcat cctgccgtgc
     1561 cggttctggg cggtgtgtct gcaggtatgt ccttgcgatg gctcgttcag tggaatagat
     1621 attctaacgt tatgcggcaa ctatctacga atcaacgtac gacacagatc gctctccttg
     1681 cagccgctcc ctccgacagg ctcactctgg tttcttatta cttacgccag acagaaaaaa
     1741 agacgttcgc tcgtacgcga aggctggaga gtgcatgcgc ctcggtagat tttgcactgg
     1801 gggccgcggc atttttttat gcttatgctt ccacgaatcc tgcaccctcg acttcggcct
     1861 ttttcttcgc aggtcccgat cgagcgggct ttcaaatcag attctaatgc aactgcaatc
     1921 agaaggtctg gatagaccat actggttccg ttcaagtccc ccataaaaca atactcgatt
     1981 ttgtcgcccg gcttctactt gacaaaaaaa tagccgtcat atatgtagct atcgtgaatc
     2041 tgcctctcac gtccatttcg ccgccaagac tcagacgcat ttcccttctg acaggagttt
     2101 cgggtatata cgccctctct cattcctgga tcagcagact ggccctcctt gggcttccca
     2161 tcactgtcat ggattgcgca gatcggtttg atccatttgc aattgctgaa gaagccgcgt
     2221 cgcaggatgt aaaaccagat gtgttgcttg agaccatcac ctgccagaaa atcgcaacac
     2281 ctctgcagtt cctcggcgct gcacgtggaa tcctgaatgc aaggagaggc ggagttgctg
     2341 tgattctttc tccgcttcgc catttcctgg acatagggga aagcgcgcat ggacgcgagc
     2401 ttcttctgga tggatttttg tcggttcttt cagagctgag cgttcgctca cgccccgtac
     2461 ttctagtaga ggcgcagcag gacgcaccgc tctttcattc agcattcgat cgaatggcag
     2521 cacttgcttc tcccatctgg cgaattttgc cggacagcgt ggaggtagca gagaacaaga
     2581 aaacgcaaac cactcgttcc gtggatcgaa tggcacaaaa taatctcgaa gggatttcgc
     2641 ctgaccgact ggctaccggt taactctcat gcctgccaat gaggcccacg cgatttccac
     2701 cgagggcttt cacctggcgc acagctcttt cagccgcact ctctatctcc gcagatgtcg
     2761 cataacgtgg aaaccaggcc actccacagg agaatgtagc atagaaatcg ccctcgcttg
     2821 aagtgtgacg aatctgcgcg aagtcgatgc gaatctcatt gaacacccgg gcagcagtag
     2881 ctgcgtccat ggagttcact atgacaccaa actcctcccc tccgagtctg cccacgaggt
     2941 ccttactcca cactctctgc ttgagtagac gacccagact ctttagaact gtatctccca
     3001 ccgggtgacc gtatgtgtcg ttgatcaact tgaacctgtc aatgtctacc atcacaaacg
     3061 gcaactctac tcctcttgct cctgccagct ccaatttcat ttccagagag tctagcatcg
     3121 cccgatggtt caatagaccg gtcaggccat ctcggaagta gagagatttg atggatcgag
     3181 ttgcttccac tctagaagca acgatcaatg caaggtagtc tggatggatc ggaagagtca
     3241 ggcaatcatc gccgcccttt gcaagagccg ccatttgcct tgctggatcg agctcgctgg
     3301 acaaaaatac aatgggaatt cgttcatacc gcggatcctg tctgagaact gtggccagct
     3361 ccattcccag gatctccgga tagtaaagat tcattagtat caattccgga tgaaacaagt
     3421 ctccggctcg tagcatttca gccggattgg agaccacatg cgtttccatt cccgcagccc
     3481 ccagaatcct ggcaaggtgc tgcgagagct caccatcgct ttccacgatc aggatccgat
     3541 acgcctggac tacaaacgga gacgttaggc gatccagcag agtaacgaga gcatccacgt
     3601 tcacaggctt agagaaaaaa ccgcttcccc caaaccttac cgaccgaaga cgcatctgca
     3661 tgtcgtcgct tcctccgaga aacacaacac agggagatgg attcaaattg cgctgaattt
     3721 cagaaatatg catctccgat tccagtcgat ccatggcagc tacaatgacg gctggaaccg
     3781 actgagccac tgcttctcgc agagtctcga gagatgcaaa tgtgcttact ccgtaaccat
     3841 agtggccgat ttgctcggcc aggtctccgc gcgcacgcgc gtctgcgtcc aggagataaa
     3901 tcagtcgatt gctcaccgac tctctgcttc gctgccgggc gtcggtttgt ccatgaaaaa
     3961 tggatcagtt tcgaattctg gccacgcgca cgagtctcct ctgaacgtcg cgcgcatcct
     4021 cccccagcat tttttgcacg gagtcctgag cctcctccca cagtggagtg catttttcaa
     4081 gcatggtgta gcctgctttt gtcaatacca gggctttctt gcgtctgtcc ttctgcgatt
     4141 cgaattgaat gagctttctg tggaccatgg gcctgaggtt tttagaaaga gtcgtgcgat
     4201 ccatcccgag tccagaagca agatcattga tcgtaagact tccatagccg gccagaacga
     4261 tcagcaagct gtactgagta gaacgaattc cggcaggagc cagggcgcga tcgtacagga
     4321 gagtaaccgc acgcgccgcg tttctgaaac tgaagttgaa gcagctcagc gcggcgcgtt
     4381 tgtattctga ctgatcaagc gacttccgct tcacgcgagg tttccctcct gccatgaatc
     4441 gacattatct gggcaacgcg ctttgcaaag tcaaaacgtt ccatatcagg gtcagtttct
     4501 acaatcctcg aaaccaattc tgtgagcgct cctccatgcc tcgcgggacg tccggttttc
     4561 aaatccacgt acgccagatc aaaccataca atggcatgca cctttcctgt ttcaggatct
     4621 cgcatgattc cttctgggaa cagacgatgt ttctcaaatc gcaaaagcct ggtctcgatt
     4681 tccaccagtt gattgagttt agccgggtac agataccgaa tggaagtgtt ctgaaccacc
     4741 cagttcttcc ccgtcttctc ggcgtccgca tacacatcat acccgtggaa atcgcgaaga
     4801 tgatccgtgc gcgcgtcaat gaaatgagaa atgtagcgtg cattgtttag atgcccgaaa
     4861 gcgtcacaat cgtggaaaga cacccggaac gttgacctgg gagtgattgc ctgaagttcc
     4921 ataaaaccct ccatccgacc aataggtgga tatacactca tttaagaagg cagaacaaac
     4981 ttgtccacaa aaaaagagtg gatatccacg tattttcaga ctgccgcaaa ctcgctgttt
     5041 ttgagcctgt aaaagcagcc agacgcaatc gccgcgcttt cgagcccata acttcgacaa
     5101 tccgcccgcc accgttccag gtccgaatga ttggcaagta gcccaagggc cttttcgtaa
     5161 aggaccgtac acaagaatcg tgcggccact atcatcaaat tctctgccag acgctcacga
     5221 ctgtcttcgc tctctatcat gcgaaaacaa tctacagatt cctccagcaa acggaactgc
     5281 tcgatcagaa aagtcgacgg agctcgcacc tgcgagtaca ggtcctccag gtattttcta
     5341 aaatttgcgc cttcccccat accagcggaa atcgcgccgg tggcgatgcg aacgtgaatc
     5401 tgactggttc cttcgtagat ggtattgatt cgtgaatctc tgaacattct ggagacgtcg
     5461 taatcttccg tataaccggc tccgccgtga atctgaacgg cgagattccc acaccgatgt
     5521 ccttcttcgg aacaataata tttcgcaacc ggcgtaagaa tcgacgcaag cgtactccat
     5581 gtcttgatgg tttcgtcgcg ccgaatgtcg cgatcctcta cgccttcctt ttccagacga
     5641 atctggtggt gctgaaacaa atcgacaatc cgcgctgtct caaaagccag tagccgcatt
     5701 gcattgacct ctctgcggat tttctgaatc atttcagcaa ctgcaggaat ctgcgcgatt
     5761 ggttttccaa actgaatgcg cgatgacgca tacttttcac attcgaagta ggcgcccgcg
     5821 ccgccgccag gcccacccgc tgcacttccc agtctcatga aattggtcat gcctgccgtg
     5881 tatctggtta agcccaggcc ttcctctccg aggatctgtc cgggcgcatc ctcatagacc
     5941 acttcgcagg tgggggaagc gtggattccc atcttactct caatgccggc tatcgttaca
     6001 tcttcgctct tgaccaggaa tacggaaaga cctcgcgcac gaccacccac ttttccagtg
     6061 cgtgccaggg tcagaagagt gcatggataa ggcccaagac cgcatccctg agaaatgaac
     6121 cgcttggtgc cattcaatcg atagctaccg tcttcacttc tgattgcagt catgcgaacg
     6181 ctgttgagat cggatccgta gtctggctct gtcaaagcca tggcgaataa cgtttcccct
     6241 gcagctgctt ttgcggcaaa ttgctcgatc tgctcttccg ttccgtatct tgccacaatt
     6301 tgcgcaaggt tcagtagagt gatggtcatg caaaaggaaa cgtccgcacg cgccagaagc
     6361 ataacaataa aagcaccgac cgttgctggt aagccaaggc cacccgcatg cctcgagata
     6421 gaataaggca tgagccccgt ttccctgaat ttttcataaa ctgagattgt ctcggaagga
     6481 aatacaacgc ggccattctg aaattggaga gccgcgcgat ccatcgcctg cgaaacctgt
     6541 gatagttctt tgccgcaaaa ttcaccgaga gactccaggc tcgaacgata gagaccaaca
     6601 gcttcttcta cactcgccgg agccatttcg aagcgagagt ttcccgtttc cagaaagcgt
     6661 ctgtgatctg cgaaatcgtt tccctccacg gcctgcacga tggaatccca atcaatcaaa
     6721 tgattgaaaa aggtctgcaa atcgggatca tcgaggaagt agttgtttgc gatcattcgc
     6781 cagaatcgca acaccccgga ggaaggacga tcaattccga acccgtcatt ctagcagacg
     6841 aacgttgttg ccgtctccaa ggatcaaatg agcgccgtca gacgcggaaa gacgcagctg
     6901 ggttgtaaac tgtgctcctg gcccgacccc atctgtcaaa ccaaggtttc catctccagc
     6961 cagggtgttg acaacctgtg tgccagggtt tagcacgcga acgtaatagc tttccgaaac
     7021 aaaaagactg aagccatcgg tagccagacc caggatggaa ccaaatcgtg ccgcagtccc
     7081 cgccgcatcc acgtgaccag ttccactgga accaggagct ggccccgcga tcgtagtaac
     7141 ctgaagtgta ttgaggtcca ctttgcgaat cttgctgtta tcagaaacaa atagattctt
     7201 accgtccgtt gtaatcgcgc ccggcgcaaa taaacgtgcc gcgtttccaa tgccatcatt
     7261 gtccagacta cacccgttgc ttccgggtag cggcccaacg atcgtcgtta cctgctgcgt
     7321 ggcgagcgag acccgacgaa tcttacaatt gccgttttcc acaacgtaga gatttgtgcc
     7381 atcagtcgtg atgccagtcg gcgcgaagaa gcgcgccgca ttccccactc cgtcggtgtc
     7441 gccaaaagtc gtagtgccgg atgacggtcc cgcaaatgtg ctaacgatgc cagtggcaag
     7501 atcaatccgt ctaattttgt tatttgttcc atcaacgaca taaagcgcat tgccagagag
     7561 agttgtcatc tgtggatcat taaaggtgga aaccgcagac gaaccgtctg cgtctccgct
     7621 gaccactgcc ccggcggccg ggcccgcgat gatggcgacg tccgcgcttc gcaaatctat
     7681 tttcttgatc ttcttgttgc caatgtctcc gccaaatgcg aatgaaccat tcgcagccca
     7741 ggcacccaga ccagagaatt tcgcggaact tcctcgcccc tccacatccc caccaagaaa
     7801 tccacttccg gcaagagtca ccacgcgccc ctgaagattc aaagggcatc cttgcatgcc
     7861 tccacccatt gcgtatcctg tacaggtcag cagaaacaac gggaaggacg ctatgctaca
     7921 gcttggatca ccagaattgc aggcgccaga gacgctgcat tgcattgtta aagaaattat
     7981 acaaatgaac agcttcaccc ggggccgtcg caacataggc ctcagattag aaaccgttag
     8041 gcgccctccg tcaacgcgag gtgcgcgaaa tgctcttcca cgggcgcgag aagcacctga
     8101 caaaaatcac aatttcattg cagacgtggc ctcatactaa aacctcgccc acctgtgaac
     8161 aaagcgtccg tcaaggctgt ctgcttgttt gttttcgtcg ccattcctgg ctgcggtcga
     8221 tccgcagggg aacctgcagt agtccagggc cagatggatc tttccaatca cgaactgctc
     8281 gatggccagc gaatcaaact ggatggcgcc tgggaattct atcacgggca attcctggtc
     8341 agcggacaat tcccacaacg tacgcttgat tttctttctc ttccgggcta ttggaacgaa
     8401 aggggagcaa cagtcgacga atacccgggc cacggttttg caacgtatcg cgcgcatctg
     8461 cgccttcacc ctcgagacgt taagcgacga ttagccctct acgtgccgca t
//
LOCUS       DAORFF010000092         8511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4019_length_8511_cov_3.470435, whole genome shotgun sequence.
ACCESSION   DAORFF010000092 DAORFF010000000
VERSION     DAORFF010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4019_length_8511_cov_3.470435"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1625)
                     /locus_tag="PKK76_05505"
     CDS             complement(102..1625)
                     /locus_tag="PKK76_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNL67957.1"
                     /translation="MDTTLRDGEQTHGVSFSAFEKLQIARFLLEQVCVDRIEVASARV
                     SRGEHETVKEIFVWAHEKGVADRIELLGFTDHKGSIDWIGEAGGRAINLLTKGSLKHC
                     REQLGKTLAQHLADIEKTVRYGEEHGFRVNVYLEDWSNGFLHSPDYVREMVGALRNLP
                     IHRFLFPDTLGIMSPDEVRAGLRDIFDLYPDIQIDFHGHNDYDLATANCLAAVSEGVV
                     GIHCAVNGLGERAGNSPLEAVVTALHDKLRVQTGVNEKALVDVSRLVETFSGKRISAN
                     HPVVGHDVYTQTAGIHADGDKKNNLYANPILPERFGRQRVYALGKLAGKASVSNNLGM
                     LGIQLPPEKELLLLDRIKQLGEQKQTVTPDDLPFLMEDLFGSGDSRQIRIKSAEIHST
                     LQGKPRAKVTLDFNGQTYEETGEGNGGYDAFMKALELIAKRVGIELPQLMDYEVRIPP
                     GGKTDALVEAMIEWAPAEGRSFRTVGVDCDQVMAAIIATEKMLNRIVQPAADLLRQF"
     gene            1729..2559
                     /locus_tag="PKK76_05510"
     CDS             1729..2559
                     /locus_tag="PKK76_05510"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNL67958.1"
                     /translation="MKLRRVSLSVLGFLLASAVFSALPRVAPLMEGLSGSGQSPGYSR
                     VPILCFHDLNGRGRYSITSATFRHFMKRLRDENIAVIPLAEVVERSQNKRLLEKPAVA
                     ITVDDDYEGAVRIAAPILREFGYPATFFVYTGGIHTDPAEGMSYDDLRRLLKEGFDVQ
                     NHSHTHTAFHVPRKGESAAEYEQRLVVEIEDSRKILEKNLMGHKIRFFAYPMGYHTPA
                     LRERVFKAGYELVVTTDARPVDLTRPFNGSFDRRTIELDGGVAELIFEQAIQIAKKAI
                     "
     gene            2644..5367
                     /locus_tag="PKK76_05515"
     CDS             2644..5367
                     /locus_tag="PKK76_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL67959.1"
                     /translation="MKTTDLVLNGGEIKIADGNSWMGAGDGINHALDDLAEIGVQELL
                     IYAQKNEVPKELIRAVKAKGLTASHIQPHNILEGGDFTPVRKHHGALLSRARKHSSAV
                     VYVGHHEPDDLLFFLAGLVLFSHRELTAIEACRTVADSDGNEAVHEALNRYAEFLREE
                     SGHSQKNGKSTVVSPSHEDADAHAFLARTAPPAATQPTDVEAVSMAAALSPREKETEA
                     PVPTPVAEPYSPPEAIEPPAPVPAVVESQKSPEKAETKPAPKPASKPAGGEIRKLDHS
                     FKPSKFTIKVKLLSIISAVIVGALTAMIFLATFFFRRDSERSIQGSNLSITEIIGLQL
                     QSEIETISYKSHLMGTTLEQPQAADQKKVFIDLFLQNNAHFMFIGVARRAGDAIIIGQ
                     SVTNDKFLKDNNISQEDITRLNQQNGKAFLASFGGATIVHNASQGFKIPLIAVSVPLA
                     RGESIVVAYLDPGTFIKAFQQTTQALPGTTAFLVDQAGDVIAHPDSKLVLSGTNLADS
                     PIIKALLESKVDNGQIRYSDKNGWNLGAYKKLSVGGLGIVSTAEENVVFGPVFAIQRR
                     NIYIMVIVVALSFIVVYLFSKTLTVPIVKLVGATQQVERGEYGIDIKPASGDEIGDLT
                     HSFVNMAVGLEEREKLKDAMGKFTNPEIAEMVLRGEMKLGGERKDAAIFFSDLRGFTA
                     MSENMEPEEVVEFLNEYFTDMVNCVDVTGGVVDKFIGDAVMAHWGALFSRGNDTENSI
                     NAAIMMRKALIAFNERPTTKSGKKKPIAMFGCGINTGPVISGQIGSETRLDYTVIGDT
                     VNLASRVESLNKPFGTDILITTDAYDRVKDVFQVEEMPAIKVKGKSEAQIIYAVLGRL
                     DDPERPKSLKELRSLVGIKFDEEKASKMEQAASGEGEVKYEVIGDKSKDAH"
     gene            5375..7510
                     /locus_tag="PKK76_05520"
     CDS             5375..7510
                     /locus_tag="PKK76_05520"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNL67960.1"
                     /translation="MQQFEVFGTRLPGQRDDWLFIGGSALVILIFGALLFWDFNRKNF
                     GGGGEVIGTITFKHRTAQRKFASQVIWDDLDTETPLYNRDAIRTAESSEAVVTLKNGT
                     KIQLDESTMIVLNISDKSADIDFAYGSVQAKQDQKAGQEVAALNIKTQDNKVISIQNS
                     DVKLSRNEGEDLSLTVQRGQANVQTSDGKTQKVEKDQKATIAAEIQVRKVVLRPTAPD
                     DGARSFITENQKAFAFAWEKPTEALTFEIASDRSFSRIITRVTTAGPGTSVALGDGSY
                     FWRLAAQDKTGKIDYSDFRRFTVIRNAPAVLLSPVSGAQFDYVTDDPLVNFTWSRSET
                     AQGYALEIAENPGFASARRIETSTTSISQKLPAGNYFARIVTTSSEAGNLPGAATAFT
                     VVRRQKTQAPLAVRPAGDRIARVILEKEGLAFSWSVDRDVRSTTLEISPNASFDQIAF
                     KGTADTNFLTVKQGFREGKYYWRLQGIDRKGNATDFSNVSSFTVAEMEKVALRGPANG
                     ADMDPVQARLEGVGLLWEGPDLSQFRVVVSPNQNLDPAVVNQMTSARAYSARNLAPGR
                     YYWKVLLVSQNGSVLTESEIRSFSILDLLQPPRLLEPPRGQTVDMTDKNELYFKWDPV
                     RGAEGYDFTLFRSYPDGRRTAILNLKTQRADFMLRDLGILDVGGFTWSVSAYGRNQVS
                     EPAASDFTITLRIDVGKPEIVTPGVQYTE"
     gene            7507..>8511
                     /locus_tag="PKK76_05525"
     CDS             7507..>8511
                     /locus_tag="PKK76_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67961.1"
                     /translation="MRALILWAGAIVFSLAPFTQRAYAQEVKTPFLEWKPIRGAAAYV
                     VQVRDTGGKVILEKTVGENKLEVELPPGKYQQRVSALNKFRKPTQYSDWVDFNIRVAE
                     LPNVETLKPTPLPVDVEKKGERAVAIEGTHLTESTKVFVDTDKGKIESTKVEVVGDNK
                     LIASFDSAKIDQGKHDIIIENPHNKIVRVENALKISEDKKVTTSSDPRVVREDAKKTD
                     RGRDTGREPGKDTGKDTGKDTGRDNGKVPAIEPMEIYPEYKPTKKQCGRAGICIPAPQ
                     KYAWNLTPQAFVPGLSRIRSGDTKGYIWPAVIGTFLVGAGYEDRHADEVAKRSQSDGL
                     G"
BASE COUNT         2311 a         2167 c         2139 g         1894 t
ORIGIN      
        1 ggaagtttat gcgaggatgg gaaacattgc gcgcggtgca aagcagagaa gctcgatccg
       61 gggaacccgg ccgttgccaa agtgcgcgcg atgtttggaa ctcagaactg acggagtaaa
      121 tcagcggcag gttgcacaat gcggttaagc attttctccg ttgcgatgat cgcggccatg
      181 acctgatcgc agtccacacc aactgtgcga aaggatctgc cttctgcagg ggcccactca
      241 atcatcgctt ccacgagcgc gtctgttttt cctccaggtg gaatgcgcac ttcgtagtcc
      301 ataagttgtg gaagctcgat tccaactctc ttcgcgatga gctcgagtgc cttcatgaag
      361 gcatcgtatc cgccgtttcc ttctcctgtt tcttcgtacg tttgaccgtt gaagtcaaga
      421 gtgactttcg cacgaggctt tccctgaaga gtggaatgaa tttctgcgct cttgattcga
      481 atctgtcttg aatcgcctga tccaaacaga tcttccataa ggaaagggag atcatccgga
      541 gtgactgttt gcttttgttc acccagctgt ttgattcgat cgaggagtag aagttccttt
      601 tctggaggaa gttgaatgcc cagcattccc aggttattgg aaacagacgc tttgcctgcg
      661 agctttccta acgcgtatac tctctgccgc ccgaatcttt ccggaagaat tggattggcg
      721 tatagattat tcttcttgtc tccgtctgcg tgaatgcccg cagtctgggt gtaaacatcg
      781 tgacccacta ccgggtgatt tgcggaaatc cttttgccac tgaatgtttc cacaaggcgc
      841 gaaacatcca cgagagcttt ttcattcaca cctgtttgca ctcgcaattt atcatgaagt
      901 gctgtgacga ctgcttcgag aggagagttc cctgctcttt cccccagacc attgactgca
      961 cagtgaattc caacaacacc ttcgcttact gccgccagac agtttgcagt ggcgagatca
     1021 taatcgttgt gaccatgaaa atcaatttga atgtccggat agagatcaaa aatatctctg
     1081 agacccgcgc gtacttcatc gggactcatg attccaagag tgtccgggaa aagaaaacga
     1141 tgaatgggga gattgcgaag agcaccgacc atttcgcgca cgtaatcagg cgaatgcaga
     1201 aatccgttag accagtcttc gaggtacaca ttcactcgaa agccatgctc ttcaccgtat
     1261 ctcactgtct tttcaatgtc agccagatgc tgagccagag tctttcccag ctgttcgcgg
     1321 cagtgtttga gagaaccttt tgtaagcaga ttgatggcgc ggccgcccgc ttcaccgatc
     1381 caatcaatgg agcctttgtg atccgtaaaa ccaagaagtt caatgcgatc tgccacaccc
     1441 ttctcatggg cccatacgaa gatctctttt acagtctcgt gctctccgcg cgaaacgcgg
     1501 gcacttgcca cctcaatgcg atccacgcat acctgctcca gtaagaagcg cgcaatttgc
     1561 agcttctcaa atgcagaaaa actcactccg tgagtctgct ctccgtcacg gagagttgtg
     1621 tccatgatct ctattcgtcg cacagggggc ctgcttatca agatagccaa aattttcaga
     1681 ttgccgccta tgtaaacgca gatcgactcc gaaaataaag ctgggcctat gaaacttcgc
     1741 agagtatccc tttccgtcct cggttttttg ctcgcctctg ctgtattttc agccctgccc
     1801 cgtgtggccc cgctgatgga aggtctttcc ggtagcggcc agagccccgg ctatagcagg
     1861 gttcccatac tctgctttca cgatctaaac ggccggggcc gttattcgat cacgagcgcc
     1921 acatttcgtc atttcatgaa aaggctacgc gatgagaaca tcgctgtgat tccactggca
     1981 gaagtggtgg aacgatccca gaataaacgg ctccttgaga aacccgccgt tgccatcact
     2041 gtggatgacg actatgaagg agccgttcga attgcggctc ccatccttcg cgaattcggt
     2101 taccctgcca cgttcttcgt ttatacaggc gggattcata cagatccagc agagggcatg
     2161 tcctatgacg atcttcgccg tctcctgaaa gaaggatttg acgttcaaaa tcattcgcac
     2221 acgcacactg cctttcatgt tccgcgaaaa ggtgaatccg cagcggaata cgaacagaga
     2281 ttagttgtgg aaattgagga ttcgcgcaaa attctagaaa agaatctaat gggacataag
     2341 attcgctttt tcgcctatcc aatgggttac cacactcccg cactgcgcga aagagtattt
     2401 aaggcaggtt acgaactggt tgtgacgaca gacgcacgtc cagtggacct tacgcgcccg
     2461 tttaatggct catttgacag gcgaaccatt gaactggatg gcggcgttgc agaattaatt
     2521 ttcgaacaag caattcagat cgcgaaaaag gcgatttagc gtcacaaaca cccgataatt
     2581 acgattgaca cgaccttttt atcgtcgtac tacctggcag ggggcggaac cttgatcgcg
     2641 gaattgaaaa cgacagacct cgtcctaaac ggaggagaga ttaagatcgc agacggcaac
     2701 tcctggatgg gagccggcga tggaatcaac catgccctcg atgacctggc ggaaattggc
     2761 gtccaggaac tgctcatcta cgcacaaaag aacgaagtcc ccaaagaact cattcgtgca
     2821 gtcaaagcaa agggactcac tgcatctcat attcagccac acaacatcct ggaaggtgga
     2881 gattttactc cagtgcgcaa acatcacggc gcgcttcttt cgcgcgcgcg aaaacacagc
     2941 agcgctgtcg tgtacgttgg tcatcatgaa ccagacgatc ttctcttctt ccttgccgga
     3001 ctggttctat tctcgcaccg cgaactaacc gcaatcgaag cctgtcgcac cgtcgcagat
     3061 tctgatggca atgaggcggt acacgaagct ctgaaccgtt atgcggaatt cctcagagaa
     3121 gaatcaggcc actcgcaaaa gaacggaaaa agcactgtcg tctctccttc acacgaagat
     3181 gcggatgctc atgcatttct cgcgcgcaca gctcctccgg cggccacgca gcctacagat
     3241 gtagaagctg tttccatggc ggcagccctc tcgcccagag aaaaagaaac ggaagctccg
     3301 gttccaactc cagtcgcaga accttactca ccgccagaag ccattgagcc tcccgcacct
     3361 gtgcctgccg tagtcgaaag ccagaaatcg cctgagaaag cagagacaaa gcctgccccg
     3421 aaacccgcgt ctaagcccgc cggcggagaa atccgaaaac tggatcattc ttttaaacca
     3481 tcgaaattca ctattaaagt gaagcttctc tccatcattt ccgcggtgat tgtgggagct
     3541 ctcactgcga tgatcttcct cgctactttc ttctttagaa gagacagcga aagaagcatc
     3601 cagggaagta acctgagcat tacggaaatc attggtctgc agctgcagtc cgaaattgaa
     3661 acgatttcgt acaaatccca cctgatgggg acaactctcg agcagccaca ggccgcggat
     3721 caaaagaaag tgttcattga tctatttcta cagaacaatg cgcatttcat gttcattgga
     3781 gtggcaagac gcgcaggaga tgcgatcatc atcggtcaaa gtgtgaccaa tgacaaattc
     3841 ctcaaagaca acaacatctc tcaggaagac atcactcgcc tcaaccagca aaacggaaaa
     3901 gcattcctgg cctccttcgg gggtgcgacc atcgtacaca acgcttcgca gggattcaaa
     3961 attccactga ttgcagtgag cgttcctctc gcacgtggcg aaagcatagt tgtagcgtat
     4021 ctcgatcctg gaacattcat taaggcgttc cagcaaacta cacaggctct cccaggaacc
     4081 accgcattcc ttgtggatca ggcgggagat gtgattgcac atccggattc aaagcttgta
     4141 ttgagcggca ctaacctcgc agattctccc atcatcaagg ctctactcga aagcaaggtc
     4201 gacaacggcc agattcgcta ttcagataag aacggatgga atctgggcgc gtacaaaaag
     4261 ctcagcgtgg gcggacttgg gatcgtatct actgcggaag aaaacgtagt atttggccct
     4321 gtatttgcga ttcaaagacg aaacatttac atcatggtga tcgtagttgc tctgtcgttc
     4381 attgtagtgt acctgttttc aaaaacgctg actgttccta ttgtaaaact agtaggtgca
     4441 acacagcaag tggaaagagg cgaatacggc atcgacatca agcctgcctc cggagatgaa
     4501 atcggggatc tgactcattc attcgtgaac atggccgtgg gtctcgagga acgcgaaaaa
     4561 ctgaaagatg cgatgggcaa attcacaaac cctgaaatcg cggaaatggt ccttcgcgga
     4621 gaaatgaagc tgggcgggga acgcaaagac gcggctattt tcttctctga tttgcgcgga
     4681 tttactgcga tgtcagagaa catggaaccg gaagaagtcg tcgaattcct caacgagtac
     4741 ttcacagata tggtcaattg cgtggacgtt acgggcgggg ttgtggataa gttcatcggt
     4801 gatgctgtga tggctcactg gggcgcactg ttttcgcgcg gaaacgacac agaaaactca
     4861 atcaatgcag cgatcatgat gcgaaaagcg ctcatcgcat tcaacgaaag accaacgacg
     4921 aagagcggca aaaaaaagcc gattgcgatg tttggctgtg gtatcaacac tggcccggtc
     4981 atttccgggc agattggttc ggaaacgaga ctggattaca cagtgatcgg tgatacggta
     5041 aacctcgcaa gccgcgttga atctttgaac aaaccttttg gaacggacat actgatcaca
     5101 acggatgctt acgacagagt gaaggatgta ttccaggtag aagagatgcc tgcgatcaaa
     5161 gtcaaaggaa aatcagaagc gcaaatcatc tatgcagttc ttgggcgact ggacgatcca
     5221 gaaagaccaa aatctctgaa agaacttcgt tcactcgtcg gcatcaaatt cgacgaagaa
     5281 aaagcatcca aaatggaaca ggctgcttcc ggcgaaggag aagtgaaata cgaagtaatc
     5341 ggcgacaaat cgaaggacgc gcactgagga catcatgcag caattcgaag tgttcggcac
     5401 ccgacttcct ggacaaagag acgactggct cttcatcgga ggcagcgcgc ttgtcatttt
     5461 gatttttggt gcgctgctgt tctgggactt caatcgaaag aatttcggag gtggtggcga
     5521 agtgatcggc accatcacat tcaagcatcg cacagcgcaa cgtaagttcg cttcacaggt
     5581 catctgggac gatctggata cggaaactcc cctctataac agagacgcga ttcgcactgc
     5641 ggaatcttcg gaagctgtag tcactctcaa aaacggaacc aagattcagc tcgacgaatc
     5701 caccatgatc gttctaaaca tttcagacaa atcagcagac attgattttg catacggctc
     5761 cgtgcaggca aagcaggatc agaaagctgg ccaggaagtg gccgctctca acatcaagac
     5821 gcaagacaac aaagtcattt ctattcaaaa ctcagacgtc aaactctcga gaaacgaagg
     5881 agaggatctc tctctcaccg tgcagcgcgg tcaggcgaat gttcagactt cagacggtaa
     5941 aactcagaaa gtagagaagg atcaaaaggc aaccattgcg gccgaaatcc aggtgcgaaa
     6001 ggtggtactt cgccccactg ctccagacga cggtgcgcga tccttcatca ctgaaaatca
     6061 aaaggcattt gcatttgcat gggaaaaacc aaccgaagcg ctcacatttg aaatcgcatc
     6121 ggacagatcc ttctcaagaa ttatcactcg cgtcacaacg gccggccccg gaaccagcgt
     6181 tgctctggga gacgggtctt acttctggag actggcagca caggacaaga caggcaaaat
     6241 cgattatagc gactttagaa gattcactgt gattcgaaac gcaccggcgg tgcttctttc
     6301 gcccgtgtct ggcgcgcaat tcgattacgt aacggatgac cctctcgtaa acttcacatg
     6361 gtcgcgaagc gaaactgcgc aaggatatgc gctcgaaatt gcagagaacc ctggctttgc
     6421 aagcgcaaga agaatcgaaa cctctaccac atcgatcagt caaaaactgc ctgcaggtaa
     6481 ctatttcgca cgaatcgtca caacgagctc agaagcgggg aatttgccgg gtgcagccac
     6541 tgcatttaca gtggtgcgaa gacagaaaac gcaggctcct cttgctgttc gcccggctgg
     6601 agatagaatt gcgcgcgtca ttcttgaaaa agaaggcctt gcgttctcct ggagcgtaga
     6661 ccgcgacgta cgcagcacta ctcttgaaat ttcgcccaat gcttcttttg atcaaatcgc
     6721 attcaaaggc acggcagata cgaacttcct gactgtaaaa caaggattcc gcgaaggtaa
     6781 atactactgg agactccagg gaatcgacag gaaaggtaat gcgactgact tttccaacgt
     6841 ctcatctttt accgtggccg aaatggagaa agtggctctc cgcggtccgg caaacggggc
     6901 tgatatggat ccagttcagg cacgtctcga aggggttgga ttgttgtggg aaggtccgga
     6961 tctttcgcag ttccgagtgg tcgtttcacc gaaccagaac ctggatcccg ccgtggtaaa
     7021 tcagatgacg tctgcacgtg catactctgc gcgaaacctc gcaccgggcc ggtactactg
     7081 gaaagtgctc ctggtttcgc agaacggttc tgttcttacg gagagcgaaa tcagatcgtt
     7141 tagcattctg gatcttttgc aacctcctcg cctgctcgaa ccgcctcgcg gacagacagt
     7201 ggacatgacg gataagaacg aactgtattt caaatgggat ccggttcgcg gcgcggaagg
     7261 ctacgacttc acgcttttcc gcagctatcc ggacggaagg cgcaccgcca tcttgaatct
     7321 caaaacacag agagcggatt tcatgctacg ggatcttgga atccttgatg tgggtggatt
     7381 cacatggtcc gtctctgcgt atggccgcaa tcaggtaagc gaacctgcag cgtctgattt
     7441 tacaatcact ctgcgaatcg atgttggtaa gccggagatt gtaacgccgg gagtccagta
     7501 cacagaatga gagcgctgat cctctgggcc ggcgcgattg tcttcagcct ggcccctttc
     7561 acacaacgag cttacgcgca agaagtgaaa acgccattcc tcgaatggaa accaattcgg
     7621 ggagcagcgg cttacgttgt gcaggtgaga gataccggcg gaaaagtaat actcgaaaag
     7681 actgtaggcg aaaacaagct ggaggtagaa ctccctcccg gaaaatacca gcagagagtt
     7741 tctgctctca acaaattcag aaagccgaca cagtactcag actgggttga tttcaatatc
     7801 cgcgtggcag aactgccgaa tgtagaaacc ctcaaaccta caccactacc tgtggacgtt
     7861 gaaaaaaaag gtgagagagc cgttgccatc gaaggaacac atctcactga atccacaaaa
     7921 gtcttcgtgg atacagacaa aggaaaaatc gagtccacaa aagtagaagt ggtcggggac
     7981 aacaaactca tcgcttcgtt tgattctgcg aaaatagatc agggcaaaca cgatatcatc
     8041 atcgaaaacc cgcacaacaa aattgtgcgc gttgaaaacg cactcaagat cagcgaagac
     8101 aaaaaggtga caacttcgag tgatccgaga gttgtacgcg aagacgcgaa gaaaacggac
     8161 cgcgggcgag acacaggaag agaaccaggc aaagacactg gcaaagatac cgggaaagat
     8221 acaggcaggg acaatgggaa agttccggcc attgagccca tggaaattta tccggaatac
     8281 aaaccaacga agaagcaatg tggacgagcc gggatctgta tccccgctcc tcagaaatac
     8341 gcctggaatt tgacgccaca ggctttcgtt ccaggcctca gtcgcattcg tagcggcgac
     8401 acgaaaggct atatctggcc tgcagtcatc ggaacgtttc tggtgggagc aggctatgaa
     8461 gatagacacg cggacgaagt agcgaagcga tcgcagtctg acggactggg a
//
LOCUS       DAORFF010000093         8480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4040_length_8480_cov_3.820890, whole genome shotgun sequence.
ACCESSION   DAORFF010000093 DAORFF010000000
VERSION     DAORFF010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4040_length_8480_cov_3.820890"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..613)
                     /locus_tag="PKK76_05530"
     CDS             complement(152..613)
                     /locus_tag="PKK76_05530"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNL67962.1"
                     /translation="MAIASGSLAPDFELIADNGVPWKLSSARGKKVLLVFYPGDDTLV
                     CTAQLCDYRDGIESFRGFQTDVVGISMNDKESHQKFKEKYDLPFPLLSDPDHKVAAQY
                     GCMSPLGTRRGVYLIDEKGIIRYGHVELMTLFKRSREELLKVLAQIPNREA"
     gene            complement(571..2304)
                     /locus_tag="PKK76_05535"
     CDS             complement(571..2304)
                     /locus_tag="PKK76_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNL67963.1"
                     /translation="MDHLNLSEKLRLYAEAFEKALECMLIADENRRVIAVNPEFERVT
                     GYMLSEVLGKRPGFLDSGNHSLRFYRGIWRSVRDTGSWQGEIQSKRKNGELYSEWLSI
                     SQLHENGHIYYLAVFSDITHRKDYERTLYALANYDTLTGLPNRNLFSEILEKEVAEAG
                     RKNEKVGLLIIDVDRFKSVVMTLGHTTADELLIDIVHRIRTCLRRHDVLARLGGDEFA
                     VILPGLTESREASSIAREILAAMSHVFTSRNYERYVTLSIGIAVYPETGIDAKFLHKN
                     ADTAAYKAKTGGRNQFRMFGFDMHTATLEQLLLEDSLRAALARKEFKLYYQPIVNTNS
                     GKLTRVEALLRWDHPDLGIIAPDYFIHLAEETGLIFQIGEWVIREACSQFRAWNKGGI
                     LMPNVSVNVSAVQLRKMNLFSIVAGAIRENSMKPFELDLEITESFAMQNMELSLGFLE
                     DLGGLGVGLSIDDFGSGYSSLAYLKRLPIQRLKIDRSFIKDMPDDRDSNAIADAIVAM
                     AHSLELGVIAEGVESARQMEYLKGIHCDELQGFYFARLRRRTKLSSQNFPDRSERLWP
                     LHLDPLRPTLN"
     gene            complement(2318..3202)
                     /locus_tag="PKK76_05540"
     CDS             complement(2318..3202)
                     /locus_tag="PKK76_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNL67964.1"
                     /translation="MAISRVRIYEVGPRDGLQNEARIVSARDKIEFIRLLSLSGLTNI
                     EATAFVRSVSQMADAQEVYTSVRDFGPRMSALVPNRKGMENALASGLREAAIFTAASE
                     TFTRKNINSSIEESFSRFKEVMQLAADHAIPVRGYVSTVVECPYEGRIAPQKVAEICD
                     RLIQAGVYEISLGETIGVAVPDDISRLLDEVTKKVPPEKLAGHYHDTRGTALSNVFRS
                     LDYGLSVFDSSAGGLGGCPYAPGASGNLATEDLLYALHRSKIETGVDLGKIADASEFI
                     RHAIAKEPASRVFAAIRK"
     gene            3254..5527
                     /locus_tag="PKK76_05545"
     CDS             3254..5527
                     /locus_tag="PKK76_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNL67965.1"
                     /translation="MMKKPLAVIAWICVALVGALGYIMVAMHRGEGLNAAWILTAALC
                     TYATAYRFYSKWIAAKILTLDDRRATPCEVHEDGRDFVRTNKWIVFGHHFAAISGPGP
                     LVGPVLAAQFGYLPGTLWILIGVVLGGAVQDFMILFSSMRRNGKSLGEMVQEEISKPA
                     GIVAMVAIVSIITILVAVLGLIVVKALAHSPWGTFTIAATIPIALFMGVYLRFIRPGK
                     VLEITTVGVILLLISVWAGKFFQQDPALGDVLTFSEPALAWMIIAYGLAASILPVWLL
                     LAPRDYLSTFMKLGTIGALALGIFLVLPPLTFPPLTRFVDGTGPVFSGDVFPFCFITI
                     ACGAISGFHSLISSGTTPKLITRESHARSVGYGSMLLESFVAIMALIAACTLSPGQYF
                     AMNASPSAVGKTPEVIVQTISGWGFPLTLQEMQVLAAELGEKTMFGRAGGAPTLAVGM
                     AHIFARLTGPALLALWYHFAIMFEALFILTTVDAGTRVGRFLLQALLGQIYKPLGNTH
                     SYGANVLGSVLLVSGWGYFLYSGVTDPLGGINSLWPLFGIANQLLSLVALCLATTILI
                     KMDKIKFLWVTLIPLTWLTAVTFTACLEKIYHPDPRIGFLSMAASLNDKLAHGQVPPE
                     KIGETGRIIFNMQLDALVAGAFLLLVGSILILSIIRWAGMLFGKGSRSLTETSPVWLS
                     NEVIAAEAAARPPWKFWMMGFFLAELFRHLSGQNRFEAARLSSDRSVERSSLAAVSAG
                     ETWAREEERRFKNPRCC"
     gene            5528..6277
                     /locus_tag="PKK76_05550"
     CDS             5528..6277
                     /locus_tag="PKK76_05550"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNL67966.1"
                     /translation="MIRTFLTAVMFLTRIPCPSWIDHSEERLNRSVAYFPLVGAIVGG
                     ICAVVFLLANRLFSREISVVLCMIAGVLVTGAFHEDGFADFCDGFGGGWTKEKILEIM
                     KDSRIGAFGTIALILLFLLKYHVMIDLNSARLPALLIAGHSISRMFPVWIMKFLPYVR
                     DVSAVTKPIGVLTHGLDMVAATLFGLVPLAYFGRLYMLIPAVASAVTALLMALYMRKW
                     IGGYTGDCLGAVQQVTEVVFYGATLAIWTFM"
     gene            6262..6777
                     /gene="cobC"
                     /locus_tag="PKK76_05555"
     CDS             6262..6777
                     /gene="cobC"
                     /locus_tag="PKK76_05555"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase family protein"
                     /protein_id="HNL67967.1"
                     /translation="MDLYVVRHPPVAVDKGVCYGQLDVALKPGFEVLGNSLRESLPSL
                     PVYSSHLSRCRQLAHLIDPDHAVDARLAEMHFGEWEGLRWDDIPRTELDLWMNDYVTQ
                     RTPGGESFADVEDRLTEFLQTLRDAGKEGAVLISHAGVIRSLLIRWTGLSPDKAFRKQ
                     IEFGSVHHISV"
     gene            7089..7607
                     /locus_tag="PKK76_05560"
     CDS             7089..7607
                     /locus_tag="PKK76_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67968.1"
                     /translation="MRRTIFILAVACASFACSAVSTSGTDKKNSDTLMANSALLLAGA
                     VLTSGPDIRGTWRDNAFMTNLNLGQAGGTWLAYGTGSGYSFTYALRAYDNSANKFYYE
                     CISQTGSGCTVGKFGAVMWTDPASNSFYWCEYAFNLTSTSAAQSASSSSAVPSNLASG
                     CNGFSWTKYDKI"
     gene            7604..>8480
                     /locus_tag="PKK76_05565"
     CDS             7604..>8480
                     /locus_tag="PKK76_05565"
                     /inference="COORDINATES: protein motif:HMM:NF033170.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LIC_13355 family lipoprotein"
                     /protein_id="HNL67969.1"
                     /translation="MKRLSCMMLVAASCSLPFAEQRKQDSAVTALPLLALLGAVSGSS
                     VSCSSLSASTPSGTAAADRITSAPGDAGSGFFDARCSIDGVRGMGDNQGSTDVFSLAA
                     TGSSSVLVLEWNGKKITNGVGADFIVFENSFNQNGNATTRFMEPVIVEVSYDGSHWCG
                     FNPIYSSGTYSQNPANWTRFAGTTNSYFNQDSNFMNATTLFTSGSSGGGGDQFDLSNL
                     VTGATSGYNCSAVASNSGCAAGDVSQLQGTSSGQGVVYVRMIAATACTNPNAGGATFP
                     QDSGAYGGGPDMDGVI"
BASE COUNT         1861 a         2084 c         2211 g         2324 t
ORIGIN      
        1 agccagattc tgatgatcgc agatggagga gagcagagac gatttgcctg aattggatct
       61 gccagtgaaa gcgacgactg gaatggaggc gggagatgga cattcttccg ctttcacgta
      121 ggatcctttg tactctactc cgcgaaagta atcaggcttc acggtttgga atctgagcta
      181 agactttgag caattcttcg cgtgaacgtt taaagagagt catcagttcg acgtgtccgt
      241 atcgaatgat tcctttttca tctatcaagt acactcctct tctggttcca agaggagaca
      301 tgcatccgta ctgcgcggcc actttgtgat caggatcgga cagaagaggg aagggaagat
      361 cgtacttctc tttgaatttt tgatgcgatt ctttatcatt catgctgatt ccaaccacat
      421 ctgtctgaaa cccgcgaaag gattcgatcc cgtcgcgata gtcgcagagc tgtgcggtgc
      481 aaacgagcgt gtcatctcca ggataaaaca cgagcagcac tttttttcct cgtgcgcttg
      541 aaagtttcca gggcactccg ttgtctgcga ttagttcaaa gtcgggcgca agggatccag
      601 atgcaatggc catagtctct ccgatcgatc aggaaaattt tgagaactca actttgtccg
      661 gcggcgaagc cgtgcgaagt agaatccttg tagttcgtcg caatgaattc ctttgaggta
      721 ttccatttgt ctggcggact ccacgccttc tgcaatcaca cccaattcga ggctatgcgc
      781 catggcaacg attgcatctg cgattgcatt gctgtctcta tcatccggca tgtctttgat
      841 gaacgagcgg tcaattttca ggcgctggat ggggagtctt ttcaaataag ccagggaaga
      901 gtagcccgac ccgaagtcat caatggacag accaactccg agcccaccga gatcttccag
      961 aaatccaagt gaaagctcca tgttctgcat tgcgaagctt tctgtgattt caaggtccag
     1021 ttcaaatggc ttcattgagt tttcgcgaat cgcgcctgct acaatggaga acagattcat
     1081 ctttctcagt tgaacggcgg agacgttgac tgaaacattg ggcatcaata ttccgccttt
     1141 gttccaggcg cgaaactgtg aacatgcttc gcgaatcacc cattccccaa tctggaaaat
     1201 caatccagtt tcttccgcga ggtgtatgaa ataatctggt gcgatgattc ccaggtcggg
     1261 atgatcccat ctgagaagtg cctctacccg ggtcagtttt ccggaattcg tatttacgat
     1321 gggttgataa tagagcttaa attcttttct cgccagagcg gcgcgaaggc tgtcttccaa
     1381 taacagttgt tccagagtgg ctgtatgcat gtcaaaacca aacatgcgaa actgattgcg
     1441 acctcctgtc tttgctttgt aagccgcagt atccgcgttt ttgtgcagga atttggcatc
     1501 aatgcctgtc tccggataga cagcgattcc aatgctcaat gtcacataac gttcataatt
     1561 gcgtgaggta aatacatggg acatggcagc caggatttct ctggcaatgc tcgaagcttc
     1621 tcgactttcc gtaagacctg ggagaatgac tgcaaactcg tctccaccca gacgcgccag
     1681 aacatcgtgt cgtctgaggc aggttcggat tctatggaca atgtcgatca ggagttcatc
     1741 tgccgtagtg tggcccagag tcatcacgac cgatttgaat cggtctacat ctatgatcaa
     1801 aagcccgact ttttcatttt tgcgtccggc ctctgctact tctttttcca gaatctctga
     1861 gaacaaattg cgattgggaa gcccagttaa tgtatcatag tttgctagag cgtataacgt
     1921 tctttcgtaa tcttttctat gggtgatatc gctgaatact gcaaggtagt aaatgtgtcc
     1981 gttttcatga agctggctga tgctgagcca ttcagaatag agttctccat tctttcgctt
     2041 gctctggatt tctccctgcc aggaaccagt gtctcgcaca cttctccaga ttcctctata
     2101 gaatctgagt gagtgatttc cggaatccag aaaacccgga cgcttgccca gaacttccga
     2161 gagcatatag cccgtgactc gttcgaactc tggatttacg gcaatgaccc tgcggttttc
     2221 atccgcgatc agcatgcatt cgagcgcttt ctcaaatgct tccgcataca accggagctt
     2281 ttcgcttaga ttcagatgat ccatgatttc acaactttca ttttctaata gccgcgaaga
     2341 cccgagaagc gggctccttt gcaatcgcat gacggatgaa ctcacttgcg tctgcgattt
     2401 tgcccaggtc tacgcctgtt tcaatcttac tgcgatgcag agcatacagc aaatcttctg
     2461 ttgccagatt ccccgacgct ccaggtgcat aggggcatcc acccaggcca ccggcgctcg
     2521 aatcaaaaac actcagtccg tagtcgagag agcggaagac attggagagc gccgtgcccc
     2581 tggtatcatg atagtgaccc gccagcttct ccggcggcac cttttttgtg acctcatcga
     2641 gaagtctcga gatgtcgtcc ggcacagcga ctccgatggt ctctcccagt gagatttcgt
     2701 agacgcctgc ctggatcaat ctgtcacaaa tttccgccac tttttgcggg gcgatcctac
     2761 cttcgtaagg gcattcgacc acagtgctca cgtatccgcg cacaggaatg gcatggtccg
     2821 cggctagttg cattacctct ttaaatcggc taaagctctc ttcaattgaa ctgttgatgt
     2881 tctttcttgt gaaagtttct gatgctgcgg tgaaaattgc cgcttcgcgg agcccggagg
     2941 ccagtgcgtt ctccattcct ttgcgattcg ggaccagagc actcattctt ggtccaaaat
     3001 ctcggactga agtgtacact tcctgtgcat ctgccatctg tgacacggat cgcacgaagg
     3061 ccgtcgcttc gatgttggtc aggcctgaga gagaaagaag gcgaatgaat tctatcttgt
     3121 cccgggcact tacaattcgt gcctcgtttt gaaggccgtc ccgggggcca acttcgtaga
     3181 ttctcactct tgagatagcc atgacgtctg ataagaatca cttgctctcc atgcagtgca
     3241 aatcaatttt cgaatgatga agaaacctct cgccgttatc gcctggattt gcgtcgcact
     3301 ggtgggcgct cttggttaca ttatggtggc catgcaccgt ggcgaaggct tgaacgcggc
     3361 ctggattttg actgccgcgc tctgcactta cgccaccgca tatcgctttt attccaaatg
     3421 gatcgcggcg aagattctca ctctcgatga tcgccgtgcg acaccgtgcg aagtgcacga
     3481 agacgggcgc gactttgttc gcaccaataa gtggatcgta ttcgggcatc atttcgccgc
     3541 aattagcggc cctggtcccc tcgtgggtcc tgtgctcgcc gcgcagtttg gttatcttcc
     3601 tggaacgctc tggattctca taggcgttgt tctcggagga gcagtgcagg attttatgat
     3661 tctattttct tccatgcgaa gaaacggaaa gtctctggga gagatggttc aagaagagat
     3721 cagcaaacct gcggggatcg tcgccatggt agcgatcgtt tctatcatta caattcttgt
     3781 cgcggtactc ggcttgattg tagtcaaagc tctcgcgcat agtccctggg gaacgtttac
     3841 gattgcggcc accattccaa tcgctctgtt catgggagtt taccttcgct tcattcgacc
     3901 aggaaaggta ctcgagatca ctacagtggg agtgattctt cttcttattt ccgtctgggc
     3961 gggaaagttc tttcaacagg atcccgcact cggtgacgtt cttacgtttt ctgagcccgc
     4021 tcttgcgtgg atgatcattg catacggcct ggccgcatcc attttgcctg tgtggctcct
     4081 tcttgctcct cgcgactatc tgagtacttt catgaagctt ggaacgatag gcgctcttgc
     4141 tctgggcata tttctggttt tacctccgct tacatttcca cccctgaccc gattcgtcga
     4201 tggcacgggc ccggtgtttt cgggagacgt gtttcctttc tgctttatta ccattgcatg
     4261 cggggcaatc tccggttttc attcgctcat atcatcagga acaacgccaa agctgattac
     4321 tcgcgaaagt catgcgcgtt cggttggtta cggaagtatg ctccttgagt catttgttgc
     4381 gattatggct ttgatcgcgg cctgtactct ttcccctggt cagtactttg cgatgaacgc
     4441 aagcccatct gctgtaggca agacaccaga agtgatcgtg cagacgattt caggctgggg
     4501 atttccactc actcttcagg agatgcaggt gctggccgca gaactgggtg aaaagacaat
     4561 gtttggtcga gcaggaggcg cacccactct ggcagtcggg atggcgcata tctttgctcg
     4621 cctgacgggc cccgcactcc ttgcactctg gtatcatttt gcaatcatgt ttgaagcgtt
     4681 gttcattctt acaacggtgg atgcggggac tcgggttggc cgattcttgc tgcaggcgtt
     4741 gctcgggcag atttacaagc cgcttggaaa tactcattcg tacggcgcca acgttctggg
     4801 aagtgtgctt cttgtgtcag gctggggata ttttctttat tcaggagtga cggatcctct
     4861 gggaggaatc aatagtctct ggccgttatt cggcattgca aaccagctcc tgtctctggt
     4921 cgccctgtgt ctggccacaa ccattctcat caagatggac aaaatcaaat ttctctgggt
     4981 gactctgatt ccgctgacgt ggctgactgc agttacgttc accgcatgcc tggaaaagat
     5041 ttatcatccg gatccgagga tcggttttct gtctatggct gcttccttga atgacaaact
     5101 ggcgcatgga caggttccgc cggaaaagat tggcgaaacc ggaagaatca tctttaacat
     5161 gcagttagac gctctcgttg cgggcgcgtt tcttctcctc gtaggttcca tcctgattct
     5221 ttctatcatt cgctgggcgg gaatgctgtt tggaaaaggg tccagaagtc tgaccgaaac
     5281 ttctcccgtg tggctttcca atgaagtcat tgctgcggaa gctgccgcca ggcctccatg
     5341 gaaattctgg atgatgggat tttttctcgc agagctattt cgtcatctgt cgggacagaa
     5401 tcgctttgaa gcagcgcgcc tttcttctga tcgatctgta gagcgttctt ctcttgcggc
     5461 ggtatctgcg ggggaaacct gggccagaga ggaagagaga aggttcaaga atccgaggtg
     5521 ctgttagttg atccgtacat ttctcactgc ggtgatgttt ctgacgcgca ttccctgccc
     5581 ttcctggata gatcattctg aagaaaggct gaacagatcg gtcgcttact ttccgttagt
     5641 cggagcgatc gtcggtggaa tctgcgcggt tgtttttctc ctcgcgaatc gtctattttc
     5701 gcgcgagatc tctgtggttc tttgcatgat tgccggtgtt ctggttacag gcgcttttca
     5761 cgaagatggc ttcgcagact tctgtgatgg gtttggtggc ggctggacaa aagagaaaat
     5821 tcttgagatc atgaaggact cgcgcattgg agcgtttgga acgatcgcac tgattctatt
     5881 gtttctgtta aagtatcatg tgatgattga cttgaattcc gcgaggcttc cggctcttct
     5941 gatcgcaggt cattctatca gtagaatgtt tccggtgtgg atcatgaaat ttcttccgta
     6001 tgtacgtgat gtgtccgcag ttacaaaacc catcggcgtt ctgactcatg gtctcgatat
     6061 ggtcgcggca acgttattcg gccttgttcc gctggcatat ttcggaagat tgtatatgct
     6121 gattcccgcg gttgcttctg cggtaacggc gttgcttatg gcgctctaca tgagaaaatg
     6181 gattggtggt tatactggcg attgcctcgg agcagtgcaa caggtgactg aagtcgtttt
     6241 ctatggagcc actctggcaa tatggacctt tatgtagttc ggcatccacc ggtagcagtg
     6301 gacaaaggcg tttgctacgg ccagctcgat gtcgcactca agcctggctt tgaagtgctg
     6361 ggcaactcac tgcgcgaaag tcttccttct ctgccggtgt attccagtca tctgagtcga
     6421 tgcagacaat tggcgcactt gattgatccg gaccatgcag tggatgcgcg cctggctgaa
     6481 atgcattttg gcgaatggga aggcttgcgc tgggatgaca tacctcgaac agagctagat
     6541 ctctggatga atgattatgt cacacagcga acgccgggcg gtgaatcgtt tgcggatgtc
     6601 gaggatcgcc tcactgaatt cttacagact ctacgggacg caggaaagga aggagcagtg
     6661 ctcatcagcc acgcaggagt cattcgcagc ttgttgattc gttggaccgg cttgtcaccg
     6721 gacaaagcat tcagaaagca aatcgaattt ggcagcgtgc atcacataag cgtataacgg
     6781 tattcggaat agagaagagt atctttcaag ttggtttttc actttgctta gtagttgacg
     6841 cagagagtta gaacgtcagt catgacttct ctccaaggga ggaagtcggg tgagtagcaa
     6901 aaatctgctg cgattcccgc gctgacgcgc aactgtgaat gcgtaaaacg catgagccag
     6961 atcttccccg tacaggtgct tcgcgaacag aggcttgtac aatgcggttc gccgcagggg
     7021 ccctggggac ggggctccgt ttgcataatc attcgaagcc tctcaatttt ttcggtggga
     7081 gacgaaatat gagaagaaca atttttattc tggcagttgc ctgcgcgagt ttcgcttgct
     7141 cggcggtttc aacttctgga acggacaaga aaaattctga cactttaatg gcaaattccg
     7201 cgttgttact cgcaggtgcg gttcttactt caggtcctga catccgcggt acctggcgcg
     7261 acaatgcatt tatgaccaac ctgaatcttg gacaagcagg tggcacatgg ttggcctatg
     7321 gaactggatc cggatattcg tttacgtatg ctttgcgtgc ctatgataac agtgcgaaca
     7381 agttctacta cgaatgcatc tctcaaacag gcagtggttg cactgtgggg aaatttggag
     7441 ccgtaatgtg gactgatccg gcctctaact cattctactg gtgcgagtac gcattcaatc
     7501 taacgtcaac ttctgccgct caatccgcaa gttcttcgag tgcggtacct tcaaatctcg
     7561 cttcaggttg caacggtttc agttggacca agtacgataa aatatgaaac gactgtcgtg
     7621 catgatgctt gtggctgcct cgtgcagtct gccatttgca gagcagcgca agcaagatag
     7681 cgctgtgacg gcactaccgc tccttgcttt gctcggggcg gtcagcggct ccagtgtttc
     7741 atgttcatcg ttgagtgcgt caacgccatc cgggactgca gctgctgatc gcataacgtc
     7801 tgccccagga gatgccggtt ccggcttttt cgatgctcgg tgttctattg acggtgtgcg
     7861 cggcatgggt gacaatcagg gatccacgga cgttttttcc ctcgccgcaa ccgggagtag
     7921 ttcagtgctg gtgctggaat ggaatggaaa aaagattacg aacggagtgg gcgcggactt
     7981 catcgtcttt gagaattcat ttaaccagaa cggaaatgcc actacccgct ttatggaacc
     8041 ggtcatcgta gaggtcagtt atgatggaag tcactggtgt ggttttaatc cgatatacag
     8101 ttccggaacg tacagtcaaa atcctgcaaa ctggaccaga tttgcaggta cgaccaatag
     8161 ctatttcaat caagacagca acttcatgaa tgctaccact cttttcactt cggggtcgtc
     8221 cggaggcggg ggagaccagt ttgatcttag caacctcgtg accggggcca catccgggta
     8281 taattgctcc gcagtagcgt ccaattccgg gtgcgcggcg ggagatgtct cacaattgca
     8341 aggtacgtcg agcggacaag gagtggtgta cgttcgcatg attgctgcta ccgcatgcac
     8401 gaatccaaat gcgggtgggg caacatttcc gcaggacagc ggtgcctatg gcggcggtcc
     8461 agatatggac ggcgtcatcg
//
LOCUS       DAORFF010000094         8464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4049_length_8464_cov_3.662504, whole genome shotgun sequence.
ACCESSION   DAORFF010000094 DAORFF010000000
VERSION     DAORFF010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4049_length_8464_cov_3.662504"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..1537
                     /locus_tag="PKK76_05570"
     CDS             20..1537
                     /locus_tag="PKK76_05570"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNL67970.1"
                     /translation="MSIATARTCYSSKGIITPEDVSRTPESRALRDRIARSTLQAGHL
                     TTRQHVHFVFAIDNASRHLVWSFLHSHPFYNSEQVSQRYVEVKPDRFYVPLSLRQPGR
                     ERHASFYMDAVQYAVGIYQELLRELDVPAAAEFYKIFPARKSRAEVWQKTVHKKSMEA
                     ARYILPTAMHTYFYHTINGLTLHRYSRLCEACDVPEEARNLVESMVRAAREIDPLFAA
                     EIPDSVPLESTPEYQFFKSYYGEESAPVSSQFIREFDESLGKRTSKLVSYDPGAVSVL
                     ASSVRAVLGATREMLSDESAVDLVLSPAKNSHLASALNETTLSRITRALQNVHYTFAK
                     KISHTADSQDQRHRMVPGSRPVLMKHFTGKPDFVSPALISSHSASREKYEEAMSVYFE
                     KIERFVDAGGTEEETVYLLPNAFPVRFYESGDLLNLHHKWKIRTCYNAQEEIFQASVD
                     ELQQAREVHPEIGKHIHAPCRIRKEAGVKPFCPEGDRYCGVRVWEQDLSEYKRVI"
     gene            complement(1534..2913)
                     /locus_tag="PKK76_05575"
     CDS             complement(1534..2913)
                     /locus_tag="PKK76_05575"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNL67971.1"
                     /translation="MRNHIGKSTRALALLLLFCVAESCKQQDSSTDAGALAPLLLGGA
                     SDAQLLGALNNAGVTALSAPPAQDPALVALGRSLFFDKELSGDRNISCSTCHSPAGFT
                     GDALSTSIGAGGTGENQLRVVSSGSMVARNAPPLFNLGAGNTRMFWDGRVSRDNATGA
                     LTTPESTLNGITPARADVASQLTSALAAQAMFPVASDVEMRGAVGNEIRAAADNQAVW
                     AALMARLVGTNNGTTGGMAAYRSLFSAAYPGVAYDDLNFGHAARAIAAFETASYAAVH
                     SPLDEFLRGNPNALSADAKAGGLLFFGRAGCGGCHRGPLLSDANFHGIAAPQLGPGAS
                     GGDDQGRFLISGSANDNYHFRTPALRNVELTAPYTHAGAYTTLAAVIAHYSDTATGLR
                     NYNGAGLRADYAATVDTNASHIQARLNARDPRVVRPLGLNATEQAQIEAFLKSLTDPS
                     ARNLSPDGN"
     gene            3082..3450
                     /locus_tag="PKK76_05580"
     CDS             3082..3450
                     /locus_tag="PKK76_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNL67972.1"
                     /translation="MRLTLQIDGSRIDVMRYSRILPLAFALGLVLTHGSGCVTPGGAG
                     GLILNYYDGPFLVTGNNVGTKRGEGTIQCFLGLACFGDGGISNIARRAQIQKISTVDY
                     EYISVISFLYTSTTIIVTGE"
     gene            3627..4154
                     /locus_tag="PKK76_05585"
     CDS             3627..4154
                     /locus_tag="PKK76_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67973.1"
                     /translation="MASGGMESAYLALSSLFSTSYTPARGCLFNSFTIPLIVTEVPLG
                     DMQFDYVTYCMFGVCAGSAKAAWREISARRVVAIHSIDVESMDAGGFSLMKVRVSGWS
                     ESAYRAAFPQKAESEMGAAAEGYNETVELLDNTVFRNVKTVIGDEAVEIQTRRGEKMY
                     FGKTEILRILNENKQ"
     gene            4151..5041
                     /locus_tag="PKK76_05590"
     CDS             4151..5041
                     /locus_tag="PKK76_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67974.1"
                     /translation="MKYLVFAVWVISCVRDVSAEDTIRLKTGEQLTGTIKSDNGTTII
                     LSTIDGDRTIERTDIYRIEFLDARRLRSSGMIFVETLSGPGVVVSPAFENLGNPAIGN
                     LSFLNYFSPNPARAQIAMLEILDTNKPRAYSRNVVFRTGYGLGETITVGLMVTSTWVR
                     INGYRGAIDDPLLRSYINFPLNPFVRVDAPNLLDYAFLVRNKQTFRASGTGGVDLKVH
                     TAKGPFTPYLDIGLFNRGGSASLGARYVFGAGVYFTAEGFTEEIATFVPARDLRGKSS
                     RYIDNNGCRFGVGLVVDSGR"
     gene            complement(5060..6025)
                     /locus_tag="PKK76_05595"
     CDS             complement(5060..6025)
                     /locus_tag="PKK76_05595"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNL67975.1"
                     /translation="MKKGTLVFNPNSGKGKAKERATNFASVWKNEFGNDLILRPTRSL
                     ADIRVAALETIQDPEMVPIFMGGDGTLSESVQGIAEHQNFQPIDRPVGLLPGGTGNSF
                     LRDFGVTNYEEARDNLIGALRSKSVQNADLAIIKYQAYKPERPDQPGSSVRRITFNIF
                     GVGIIADITEMAVKMRWMGAANYTVASLWKIFSHNLYNFEITIDGKREDMACNMISVH
                     NSQYTGGAMHIAPSVRVNDGQLFYLVPRYVGKLTLLKKFPELFKGTHVNSDQMRTGFV
                     KNIGIRHAHPMWMNVDGELERGWNPDLSIQPNFWKIYMMAERMLP"
     gene            6076..6939
                     /gene="rsmH"
                     /locus_tag="PKK76_05600"
     CDS             6076..6939
                     /gene="rsmH"
                     /locus_tag="PKK76_05600"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNL67976.1"
                     /translation="MEFVHVSVMPEEVISWAPQDARLIVDGTAGEAGHTLLLARRCPN
                     ARILAFDRDPEMLERARKKLGLAGMENRVELIRGSFGEIPEHTREADFILLDLGVSMH
                     HFRGAGRGFSYTDDSLDMRLDPDLEKTAADIINHSSEKDLKLIFQNYGEERFAGRIAR
                     AIVQKRPVRTARDLSSIILGSVPRSRSHPATRVFQALRIAVNDELGEIERALPLLARS
                     LAPGGRLAVISFHSLEDRIVKHGLRALDLKILTKKPLEPTEEEVRANPASRSARLRVA
                     EKPADNAGEGP"
     gene            6936..7262
                     /locus_tag="PKK76_05605"
     CDS             6936..7262
                     /locus_tag="PKK76_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67977.1"
                     /translation="MKRGGIFQLLLVLAVFLVPFGVVHFYTRNIVSASLLRRDIHLAG
                     LKKEQLVKKNMALKEALGRLTGRDLVLPSEGLPFSENNRIVRLRMSRDSEAAETRSVP
                     DQPALN"
     gene            7264..>8464
                     /locus_tag="PKK76_05610"
     CDS             7264..>8464
                     /locus_tag="PKK76_05610"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNL67978.1"
                     /translation="MKLIDICSGECVCPPGYAQIEIIRITDDSRDARPGVLFFGTPKS
                     TYLAAAKSAGAITVGEPGSGADIEVENVSACMGRLSSRFFGDPSLTVHVAGITGTNGK
                     TSTAWMVYHLWRASGIKAGLIGTLGVRYDSPEGEVSLNTGYTTPRSYQIQELLRKMKT
                     AGVTHCVMEVSSEALALGRLTGTRFAVASFTNLSRDHLDFHGTMEEYFSAKKLLLAQA
                     AQAGGRLVVYESQDRSEEGKFSRRMSDYASSLGARVKVLTEPVAFGFAASFQNINATI
                     AIHTAFDSGPECEDALRNVSKIPPVPGRFEPVDIGAAAPGSIAIVDYAHSPDALATLL
                     TEAHATGRYVIVVFGCGGNRDPGKRPIMGEIAGRLADFAIITEDNPRKEEPALIRSQI
                     HAGVRVLD"
BASE COUNT         1927 a         2179 c         2228 g         2130 t
ORIGIN      
        1 aaccgacgta ccttttgatt tgtctatcgc gacggcacgg acctgctatt cttccaaagg
       61 aatcatcacc ccggaagacg tttcgagaac tcctgaatct cgcgcactca gagatcgcat
      121 cgcgaggtcc actctgcagg cggggcacct gacaacgcgc cagcatgtgc actttgtctt
      181 tgccatagac aacgccagtc ggcaccttgt gtggagcttc ctgcattcgc atccgtttta
      241 caattcagaa caggtcagcc agcgttacgt ggaagtgaag ccggatcgtt tttacgtgcc
      301 actttccctg agacagcccg gtagagaaag gcatgcgtcc ttttatatgg atgcggttca
      361 gtacgcagtg gggatttacc aggaactact tcgagaactc gatgttccgg ctgcggctga
      421 gttttacaag atctttccgg cgcgcaaatc cagagcagaa gtctggcaga agactgttca
      481 caaaaagtcc atggaagcgg cgcgctatat tctgcccact gcgatgcaca cgtacttcta
      541 tcataccatc aacggattga ctctgcatcg ctacagcagg ttgtgcgaag cttgcgatgt
      601 tccagaagaa gcgcgcaatc tcgtggagtc catggtccgc gctgcgcgcg aaattgaccc
      661 gttattcgcc gctgagatcc ccgactccgt tccgctggaa agcactccgg aatatcagtt
      721 ttttaaatca tactacgggg aagaatctgc gcctgtttct tctcagttca ttcgtgagtt
      781 tgatgaatca cttggaaaga gaacttctaa gctggtgtcc tatgatccag gtgccgtttc
      841 tgttctagct tcttccgttc gcgccgtgct cggcgcaacc agggaaatgc tgtctgatga
      901 atctgcagtg gatcttgtat tgagtcctgc gaagaattcg catctcgcca gtgcactcaa
      961 tgaaactact ctttctcgca tcactcgagc cttgcagaat gtgcactaca cttttgcaaa
     1021 gaagatctcc cacaccgcag attcgcagga ccagcgtcat cgtatggtgc ccggttctcg
     1081 tccagttctc atgaagcatt tcacagggaa gccagacttt gtttcgcctg ctctgatttc
     1141 ctctcattct gcgtctcgcg agaaatacga ggaagcaatg agtgtctact tcgaaaagat
     1201 cgagcgattc gtagatgctg gcggcacaga agaagaaact gtgtatcttc tccccaatgc
     1261 gtttccagta cgcttttacg agtcaggcga tttgttgaat ctgcatcaca aatggaagat
     1321 tcgcacgtgc tacaatgccc aggaagagat cttccaggct tccgtagacg aattgcagca
     1381 ggcgcgcgaa gtgcatccag aaatcggcaa gcacatccac gcgccgtgca gaattcgaaa
     1441 agaagctgga gtaaaaccat tctgcccgga aggagaccgc tactgcggtg ttcgagtctg
     1501 ggagcaggat ctttccgaat acaaaagagt catctagttt ccatccgggg aaaggtttcg
     1561 cgcagaaggg tctgtcagac tttttaagaa tgcctctatc tgcgcctgct cggttgcgtt
     1621 gagtccaagc ggacgtacca ctcttggatc tcgcgcattc agacgagcct gtatgtggga
     1681 tgcattcgta tccactgtgg ccgcataatc tgctctcagt cccgctccat tgtagtttcg
     1741 cagaccagtc gcagtgtcgc tgtagtgcgc aatcaccgcg gcaagggtcg tgtatgctcc
     1801 agcatgagtg taaggagccg tgagctctac gtttcgaaga gccggtgtgc gaaagtggta
     1861 gttgtcattt gcagatcccg aaatcaagaa tctaccctga tcatctccac cggaagcacc
     1921 cgggccaagc tgaggtgctg cgattccgtg aaagttcgcg tcagacagaa gcggtccgcg
     1981 atggcatcca ccgcatcctg ctcggccaaa gaatagaagc cctcctgctt tcgcatccgc
     2041 actgagagca tttggatttc ctctcaggaa ttcatccagg ggcgagtgga cggcggcgta
     2101 gcttgccgtt tcgaatgcag cgatggctct cgccgcatgg ccaaagttta gatcatcata
     2161 cgcaacaccg gggtaggctg cactgaacaa ggaacgatag gcggccattc cgcctgttgt
     2221 cccgttgttt gttcccacaa ggcgtgccat gagggccgcc cagaccgctt gattgtcagc
     2281 ggcagctctg atttcatttc caacagctcc tcgcatttct acgtccgatg ctacaggaaa
     2341 cattgcctga gcagccagag cagatgtgag ctgagaggcg acgtctgctc tagctggagt
     2401 gatgccgttt aacgttgatt cgggagtggt cagagctcct gttgcgttgt cacggctcac
     2461 tctgccgtcc cagaacattc gcgtattgcc agcccccaga ttgaagagag gcggggcatt
     2521 tcgcgcaacc atggatccag atgaaacgac gcgaagttgg ttttcgcctg ttccgcctgc
     2581 accaatggaa gtagagagtg cgtctccagt aaaaccagcc gggctgtgac atgtggagca
     2641 cgatatgttt ctatctccgg acagttcttt gtcaaagaag agactgcgcc cgagcgcaac
     2701 gagagcagga tcctgggcgg gcggagcact gagtgcggtg actccggcgt tattcaacgc
     2761 tccgagaagc tgtgcatcgc ttgcgcctcc gagaagcagc ggagcaagcg ctcctgcatc
     2821 tgttgagcta tcctgctgct tgcagctttc tgcaacgcag aacaatagta agagtgccag
     2881 tgctctcgtg ctttttccaa tgtgattccg catggcggac ctccacggtg cgacccggta
     2941 atttgggcct accacgagat aaccatgtgg atggattgat ttgcgattaa tccctctttt
     3001 ttcccaaaaa ctcacgccaa aaaatcgcca tttcttgtcg aaaaatgact ctaaaataaa
     3061 aatcacagag ttttcttcgt catgcgcttg acgttgcaga ttgatggttc tagaatcgac
     3121 gttatgcgct attctagaat tctaccactt gcattcgccc ttggtcttgt actcacccat
     3181 ggtagcggat gcgtgacacc aggtggcgcc ggtggactga tcttgaacta ctatgacggt
     3241 ccgtttctag taactggaaa caatgtagga acgaagaggg gagagggaac cattcaatgt
     3301 tttcttggtc ttgcctgttt cggagatggt ggtatctcga acattgctcg tcgggcacag
     3361 attcagaaaa tctccaccgt agattacgaa tacattagcg ttatttcctt tctctataca
     3421 tccactacga tcatagtcac gggtgaatga tgcctcgttt tttcagtttg atcctgcgct
     3481 gtctcttccc ggcgtatgga tttcttgtcc tttcgtttgt ttctttcccg agttgcagca
     3541 cggtgcttct tccgccgttc aatgatgccc cctttgtcgg aggtcgacaa tcgggcgcca
     3601 caccttacag cacccgggct cgatccatgg cttcgggcgg aatggagtct gcctatctcg
     3661 ctctctcttc tctgttcagc accagttata cacccgcccg tggatgtctg ttcaatagtt
     3721 ttacaattcc attgatcgtt acagaagtcc ctctcggtga catgcagttt gattacgtta
     3781 cctactgcat gttcggggtc tgtgctggaa gtgcaaaggc cgcctggcgt gagatttcag
     3841 cgcggcgcgt agtcgcgatt cattcgatcg acgtggagag tatggatgca ggcggtttct
     3901 ctttgatgaa agtgcgagtg tccgggtgga gcgagagtgc ttatcgggct gcctttcccc
     3961 agaaagctga gagcgaaatg ggggcggcag ctgaggggta caacgaaaca gtcgaattgt
     4021 tggacaatac tgtctttcgc aacgtcaaga ctgtcatagg tgatgaagca gtcgagatcc
     4081 aaacacgtcg cggagaaaag atgtatttcg gaaagactga aattctgcgc attctcaacg
     4141 agaataaaca atgaagtatc ttgttttcgc cgtgtgggtc atttcttgcg tgcgagatgt
     4201 tagcgcagaa gatacgattc gcctcaaaac cggcgagcaa ctcaccggaa cgatcaaatc
     4261 ggacaatggc acgacgatta tactttccac aattgatgga gatcgaacga tcgagagaac
     4321 tgacatttac agaattgagt tcctggatgc gagacgcctc cggtcgagcg gaatgatatt
     4381 tgtagaaacc ctgtctggac cgggcgtggt tgtttctccg gctttcgaaa acctgggaaa
     4441 tccagccatt ggcaatttat cgttcctgaa ctacttttcg ccaaaccctg cgcgcgcgca
     4501 gatcgccatg cttgagatcc tggacacgaa caaaccgcgg gcttactcgc gcaatgttgt
     4561 gttcaggact ggctacggac tgggagagac aataacggtc gggctcatgg ttacgagcac
     4621 ctgggttaga atcaacggat acaggggagc cattgacgat cccctgctga gatcatacat
     4681 caatttccct ctcaatcctt ttgtaagagt agacgcaccc aatctgcttg attacgcctt
     4741 tttagttcgt aacaaacaaa cgtttcgcgc cagcggcact ggcggagtcg atttgaaggt
     4801 ccatactgcg aaaggtccgt ttacacccta tcttgatatc ggcctgttta acagaggcgg
     4861 cagcgcatcg ctgggtgcgc gctacgtttt tggagcgggc gtgtatttca ccgccgaagg
     4921 cttcacggaa gaaattgcca cgtttgttcc cgcccgggac ttgcgcggca aaagctcgcg
     4981 atacattgac aacaatgggt gccggttcgg tgttgggctc gtggttgatt ccggccgtta
     5041 ggcgccactg agagctccct cagggcagca tgcgttctgc catcatgtag atcttccaga
     5101 agtttggttg aatcgaaaga tccggattcc aaccgcgttc cagttctccg tccacgttca
     5161 tccacattgg atgggcatgt ctgatcccaa tgttctttac aaatcctgtt cgcatttgat
     5221 ctgaatttac atgggttcct ttgaatagct ctggaaattt tttcagcaga gtgagtttgc
     5281 ccacgtatct cggaacaagg tagaaaagtt gtccatcatt gactcgaaca gacggcgcga
     5341 tgtgcatggc cccgcctgtg tactgagaat tgtgcacgct gatcatattg catgccatgt
     5401 cttctctctt gccatcgatc gtgatttcaa agttatacag attgtgcgaa aagatcttcc
     5461 agagactggc tactgtgtag ttggccgctc ccatccacct catcttgaca gccatttccg
     5521 taatatccgc aatgataccc acaccgaaga tattgaacgt tatgcggcga acagaggatc
     5581 cgggttgatc gggtctctcc ggtttatatg cctggtattt gatgatagca aggtccgcgt
     5641 tctgaacaga ctttgagcga agagctccaa tcagattgtc tcgcgcttcc tcgtagttcg
     5701 taacaccgaa atcgcgcagg aagctgtttc cagtcccacc gggcaaaagg ccaaccggtc
     5761 gatcaattgg ctggaagttc tggtgctccg cgattccctg cacactctcg gacagagtcc
     5821 cgtctccgcc catgaatatg ggaaccattt caggatcctg gatggtttca agggcggcca
     5881 cgcggatgtc ggccagggat cgagtagggc gcaaaatcaa atcattgcca aattcgttct
     5941 tccagacgga ggcgaagttc gtggcacgtt cttttgcttt tcccttgccg ctgttcggat
     6001 tgaagaccag tgtgcccttt ttcatggtga atcagggggc ttagttaaac ctccgtgtca
     6061 atctaatatc ttcgcatgga atttgtacac gtatcagtca tgccagagga agtcatttcc
     6121 tgggccccgc aggatgcccg tctcatcgta gacggaaccg ctggagaggc cggtcatacg
     6181 ctccttcttg ccaggcgctg tccgaacgcc cgcatccttg cattcgatcg tgatccagag
     6241 atgctcgagc gagcccgtaa gaagcttggg ttagccggga tggagaaccg cgtagagctc
     6301 atccgtggtt cgtttggtga gatcccggaa catacgcgag aagcggattt cattttgctc
     6361 gatctgggtg tatccatgca tcattttaga ggggcaggca gaggcttttc ctacaccgac
     6421 gattcgctgg acatgcgcct tgaccctgat ctggagaaga ctgccgcgga catcatcaat
     6481 cactccagtg aaaaggacct gaagctgatt tttcaaaact atggagaaga aagatttgca
     6541 ggtcgaattg caagggcgat cgttcagaaa aggcccgttc gcacggcccg cgatctttct
     6601 tctataatac tgggcagcgt tcccaggtcc aggtcgcatc cggccacaag agtcttccag
     6661 gcccttcgaa ttgcggtgaa cgatgaactg ggagagatcg aaagagccct tcctcttctc
     6721 gcccgtagcc ttgcccctgg cggaaggctg gctgtgatat cctttcattc tcttgaggac
     6781 agaatcgtca agcatggcct gcgtgccctt gacctcaaaa tcctgacaaa gaagcctctt
     6841 gagcccacgg aagaggaggt gcgtgcaaac cctgcttcca ggtctgcgcg gctcagagtg
     6901 gcagagaaac ctgccgataa tgcaggcgag ggtccatgaa acgcggcggt atttttcagc
     6961 ttttgcttgt tctggctgtg tttctggttc cgttcggggt cgttcatttc tatacccgca
     7021 acatcgtatc tgcctccctg cttcgccgtg acattcatct ggccggactg aagaaagagc
     7081 agcttgttaa aaaaaacatg gcgctgaagg aagccctggg gagactcacc ggcagggacc
     7141 tggttttgcc ctcagaaggc cttccgttct ctgagaacaa tagaattgtg cggcttcgca
     7201 tgtctcgcga ttctgaggcc gcagaaacac gctcagtgcc agaccagccc gcattgaact
     7261 gaagtgaaac tgatcgatat ctgctccgga gagtgcgtct gtcctcccgg atatgctcaa
     7321 atagaaatca tacgtattac ggatgattcg cgcgatgccc gtcctggtgt cctttttttt
     7381 ggaacaccta aatccacata ccttgcagct gcaaaatcgg cgggcgcgat cacggttggt
     7441 gagcctggaa gtggcgcgga tatagaagtg gagaatgtgt ctgcctgcat gggcagactt
     7501 tcttccaggt ttttcggaga tccatcgctt acagttcacg tcgcaggcat cactggaacg
     7561 aacggaaaga catccactgc ctggatggtg tatcatctct ggagagcatc tggaatcaag
     7621 gcgggcctga ttggaactct gggagtcaga tacgatagcc cggagggaga ggtttcgtta
     7681 aacaccggtt atacgactcc aagatcctat caaattcaag aattgcttcg caagatgaaa
     7741 accgccgggg tgactcactg tgtgatggaa gtttcatcgg aagctctcgc tctgggtcga
     7801 ctgaccggga ccaggtttgc cgtagcttct ttcactaacc tttcgcgtga tcatctggac
     7861 ttccacggca ccatggaaga atatttttct gcgaagaaac ttctcctggc acaggctgct
     7921 caagctggag gtcgcctggt ggtgtatgaa tcgcaggatc gctccgagga aggaaaattt
     7981 tccagacgca tgtcggacta cgcaagctcc cttggagcgc gcgtgaaggt tttaacagaa
     8041 cccgtggctt ttggctttgc cgccagcttt caaaatataa atgctacgat tgccattcac
     8101 accgcttttg attccgggcc tgaatgtgaa gatgcactaa gaaatgtttc taaaattccg
     8161 ccagtacctg gtcgatttga gcctgtggac attggcgccg cggctcccgg ttcgatcgcc
     8221 attgtggact atgctcattc ccccgatgca ctagctactt tgcttactga agcacatgca
     8281 accggtcgct atgtgattgt tgtttttgga tgcggcggga acagagatcc agggaagcgc
     8341 cctatcatgg gagaaattgc aggaagactt gcggattttg cgatcatcac agaagacaat
     8401 ccgcgaaaag aagagcccgc attgatccgt tcccagattc acgccggagt cagagtgctc
     8461 gacc
//
LOCUS       DAORFF010000095         8430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4089_length_8430_cov_4.792478, whole genome shotgun sequence.
ACCESSION   DAORFF010000095 DAORFF010000000
VERSION     DAORFF010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4089_length_8430_cov_4.792478"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2048)
                     /locus_tag="PKK76_05615"
     CDS             complement(<1..2048)
                     /locus_tag="PKK76_05615"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNL67979.1"
                     /translation="MLKHDYPDASEIRRMIAEGQFHRTLQIEAIPPLVEVRSHQRGPV
                     LILERAAGHLLPTILEVGRLNTPRFLHLASSIARAVADLHGANVIHRGLSPELLLCDE
                     NKRKCYILDLSPASRLSRMNREIEKSIPMTGNPGYIAPEQTGRINRSVDHRTDLYSLG
                     IIFYQMLANRHPFVAHAPAEWAHAHIARAAPSIDRKDIPPMLLRIVTKLISKNAEDRY
                     QSALGLIEDLDRCSDELLRKGEITEFTPGEKDVSSLLHMSEKLYGRAHELDILLRAFE
                     ASRTGPLQMVLVTGSAGVGKTALIGELQKKIAESKGLFVEGKFEQYERHSPYKAWVAA
                     SQKLVERTNADAPTLAARTRERIKENVGGALGVLMESIPALRAWFNTVQEPVELPPAE
                     SRNRFTDTFVNFTTAFDQTDRPLVIFLDDVQWADAGSLDLLRSFYSIASDARILIILA
                     CRDTDVDTISSFASTLEELKKQKTFRIAKMSPLLREDVNQLIADSLLLSSDRTSALAS
                     WVHEQTEGNPLYATQLLHTLHQEGTLYFDQTAARWLWNESRLRELRDNVDLAEFLARK
                     IGGLGKDTREILSVAACLGNRFDFALLRACSGIAEEDVKAEFYPKFEPSRGAACRAAI
                     RRSDAPAHSRPDPRQSALARQFHPRRQRRRAGSTGKLAVAGELRDLSAAVGRNRCRQD
                     T"
     gene            2241..3341
                     /locus_tag="PKK76_05620"
     CDS             2241..3341
                     /locus_tag="PKK76_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNL67980.1"
                     /translation="MNSSITDPVSFFLLSSGAVFALVWALGQCLERRTGSLSYLSAAT
                     LASAGLWLLSGAHFFSGLYQAFPHLLLVHLPVVACIGPCLYFYYRKLIHPSIPIRPRD
                     VAHFVPAILVAVALIPFYSLSPGEKIEIATGIRKDLLAKWFGVLNAFPKLSAIGYLSA
                     FAIRNRFLLSLRAPLNHVQLFVILFGCWIVFGTGLGLFGMILQTPSLQRWSAWTLPLI
                     IFALYLVSKRYPEFISELQVEVKRAPYGKSTLTRLDVDRIIENLQSYMKSERPFLDED
                     LKLADVADAMHITPHQLSEILNERLGRNFARFVNQYRIEEARRLLLEESDRTVLSIAL
                     AVGFNSKSAFNGAFLDFCGVTPSQFRSGQSPK"
     gene            3448..5319
                     /locus_tag="PKK76_05625"
     CDS             3448..5319
                     /locus_tag="PKK76_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNL67981.1"
                     /translation="MNYKNLAEMFEASSVRFSNRPAFAWYDAHNQLHEKSYGDLYRDA
                     TYLARALVVLGVESREHVGLLSDNRYEWMVADWAVIFCGAADVPRGADITDSEITYIL
                     SHAQIKILFLENEKLFERIQNLRHSLPLLKSVIVMDPGARENPEFTRMQDLIIRGADL
                     LPESVEERVAQIRPEHLFTLIYTSGTTGAPKGVMLTHANMCSQISRMPFVPRPGDRIL
                     SILPVWHIFERVFEMIAIAGGACTYYTNVRNLREDLKRVKPSFMASAPRLWESIYSGI
                     QSNVQKASGFTRFLFRSAIFLAREVRGGIRFLRGGELDLRGRNPLISFVRALWTLFKI
                     VCLMLPFAVLDFLVLRKIRAATGGHLRGSVSGGGALPLHIDTFFNDIGIPVLEGYGMT
                     ETSPVIAVRTWKSLVPGTVGPLYPDTHIRIVDPASGARVFDSQNPKVGRGKKGEIHVR
                     GPQVMAGYFKNPEATAGVLSDGWLNTGDLGMMTFNNCLKITGRSKETIVLMGGENVEP
                     TPIESRLLQSAFIDQVMVVGQDRKTLGALIVPNRSAFDNEEYERIVASMEAQATIRKE
                     VQSLINAESGFKSFERVVVFSLVPGPFVVGDELTAKLSLKRHRIAEKHAELIESMYS"
     gene            complement(5369..6124)
                     /gene="tpiA"
                     /locus_tag="PKK76_05630"
     CDS             complement(5369..6124)
                     /gene="tpiA"
                     /locus_tag="PKK76_05630"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNL67982.1"
                     /translation="MRKKLIAGNWKMFKKIGEAQSLIKEIAQGAGDLGGVELLVCPPY
                     TALNAASEARKGTQIKVGAQDLHWEEQGAFTGKISWDMIRDTGAEYVIIGHSEQRTYF
                     HETDDTVNKKTKTALKGGLIPVICVGETLAERDGNRTMDVVSTQTKKAFEGITKEDAT
                     KCVIAYEPVWAIGTGRNATPEQAQEVHAGIRALIQSLYDASVAEGMRILYGGSMKPDN
                     AKSLLTQKDIDGGLIGGASLKADSFLAIARAGL"
     gene            6274..6486
                     /locus_tag="PKK76_05635"
     CDS             6274..6486
                     /locus_tag="PKK76_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67983.1"
                     /translation="MLHEYEDMDENYRSANFLGRKSLIATVKSDTLLPENGLRARSLL
                     SEAQFARMEVIRQRTLNKVYARFERD"
     gene            complement(6518..7714)
                     /locus_tag="PKK76_05640"
     CDS             complement(6518..7714)
                     /locus_tag="PKK76_05640"
                     /inference="COORDINATES: protein motif:HMM:NF020588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HNL67984.1"
                     /translation="MAHPIASCAEAEAFFFKYVSANDVGATGSHQAGFYMPRDSWKMF
                     FHEPGERGENRDTWLHVQWDHGTTKSRFVWYGKKTRSEYRLTNGFQFLSPDNVGDLLV
                     LAKQETNYYGFILQSDESIDEFLASFGLSPSDSGTVQFRRPSDDASLEEVIHPWARTF
                     GGEFPSGEMLSSKAREFVRPLLAPPKSEIPVTAQSKGLDLDSLLLQWLDYEFRIFRII
                     EREKYGSLLARPFESVEDLIQISHTILNRRKSRAGNSLENHLRAIFQFLGIRFSFQET
                     TEGSRRPDFTFPGKYYYDNPAFPSSALRFLGVKTTCKDRWRQILNEADRIPRKHLFTL
                     QQGVSSTQLSEMWDCGVILVVPQKHKPFFPPAFQSKIMSLREFLDELPEIPEGAEMLS
                     KEYKLF"
     gene            complement(7714..>8430)
                     /gene="dcm"
                     /locus_tag="PKK76_05645"
     CDS             complement(7714..>8430)
                     /gene="dcm"
                     /locus_tag="PKK76_05645"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HNL67985.1"
                     /translation="FSIAGVSKKKSLGREHGFRDRTQGTLFFKIAEILEARRPRAFLL
                     ENVKNLLSHDQGRTFQIIRETFEELGYTLFHKVLDAQGYVPQHRERIFLVGFDSNVYP
                     APVFSFPDPPQQIVQLKSILEKSVHQKYTLSAHLWKWHQDYAAKHKKNGNGFGYGLVN
                     GDSVARTLSARYYKDGAEILVSQNPVPRRLTPRECARLMGFPDSFRIAVSDTQAYKQF
                     GNSVAVPLVTAVARSLSRAL"
BASE COUNT         1939 a         2071 c         2151 g         2269 t
ORIGIN      
        1 gtgtcttgcc ggcaccggtt tcgccccaca gcagcagaca ggtctcgcag ctctccggcg
       61 acagccagct tgccagtgct gccagcgcgt cggcgttgcc gccgggggtg aaattggcga
      121 gcgagggcgg attgtcgggg atcaggtcga gaatgagctg gcgcatcgga gcgacggatg
      181 gcggcacggc aggcagcgcc acggcttggt tcaaatttcg ggtaaaattc cgcctttaca
      241 tcttcttccg caattccgga gcacgctcgc agaagagcaa aatcaaaacg gtttcccagg
      301 caggccgcga cagacaggat ctctcgcgta tcttttccaa gccccccgat ctttcgcgca
      361 agaaattctg caagatccac attgtctcgc agttccctga gcctggattc attccatagc
      421 cagcgcgccg cggtttgatc aaaatagaga gttccttcct ggtgcagagt gtgcagaagc
      481 tgcgtcgcat agagaggatt gccttctgtt tgttcgtgga cccagcttgc cagagcagac
      541 gtgcgatcgc tcgagagtag aaggctatca gcgatcagct ggtttacgtc ttcgcgcaag
      601 agaggggaca tctttgcgat gcgaaaggtt ttctgtttct tgagttcttc cagagtggat
      661 gcgaaactgg aaatcgtatc cacatctgta tctctgcatg cgagtatgat gagaatgcgc
      721 gcatcggatg cgatcgagta aaaggagcga agtagatcca atgatccggc atccgcccat
      781 tgcacgtcgt cgaggaagat caccagaggc ctgtccgttt gatcgaacgc agtcgtgaag
      841 ttcacaaacg tatctgtaaa acggtttctg gattctgcag gcggaagctc cactggttcc
      901 tggactgtgt tgaaccacgc tctgagcgct ggaatgctct ccatcagaac tcccagagcg
      961 ccgcccacgt tttctttgat cctctctctg gtccttgcag caagcgttgg cgcatctgcg
     1021 ttggttcttt ccaccagttt ttgagaggcg gccacccacg ccttgtaagg cgaatgtctt
     1081 tcgtattgtt cgaacttgcc ctctacaaat agaccttttg actccgcaat cttcttctgc
     1141 aattctccaa tgagtgccgt cttcccaacg cccgcgctac ctgtgaccag aaccatttgg
     1201 agtgggccgg ttcgcgaagc ctcgaaagcg cgaagaagta tgtccaattc atgtgcgcga
     1261 ccgtacagtt tttcagacat gtgaagcaaa gaggatacgt ctttttctcc cggagtaaat
     1321 tctgtaattt ctccttttcg cagaagttca tcggagcagc ggtccaggtc ttcgattaat
     1381 cccagtgcgg actgatagcg atcctccgca ttttttgaaa tcagtttcgt gacgattcgc
     1441 agaagcatgg gaggaatgtc tttgcgatcg atagatggcg cggcgcgtgc aatgtgagcg
     1501 tgcgcccatt ccgcaggagc gtgtgctaca aaaggatgcc tgttggccag catttgataa
     1561 aagatgatgc caagtgaata gagatctgtg cgatgatcca cactgcgatt gattctccct
     1621 gtttgttcgg gagcgatgta tccaggattt cctgtcatcg gaatgctctt ttcaatttcg
     1681 cgattcattc gcgagagtcg cgatgccgga ctcagatcca ggatgtaaca cttgcgttta
     1741 ttctcgtcgc ataacagaag ttcggggctg agtccgcgat gaataacatt ggctccatgc
     1801 aaatctgcca cggcgcgcgc gatcgaagaa gccagatgca aaaatcgcgg cgtattcaat
     1861 cggcccactt cgagaatggt tggcagtaga tgccccgcag ccctctccaa tatgagaacc
     1921 ggtccgcgct ggtgagatcg gacttctaca agagggggaa tagcttctat ctggagtgtg
     1981 cgatgaaact ggccttctgc aatcatacgc cggatttcgg atgcatccgg atagtcatgc
     2041 ttcagcatct tgatgagtac tggctttctt tgatctttga ggaatccttc ataaatgatg
     2101 gctttctcta attctgtaag aagttcgccg gtgatgatgc caggaaaatt cagaaggacc
     2161 tccccaaaag ttcagcctgt ttcaaattca caaatgcaca gcggttgacg aaaagtgtcc
     2221 accatagttg gcttgtccca ttgaattcgt ctatcacgga ccctgtcagc ttttttcttc
     2281 tgtcaagtgg tgcggttttc gcgctcgtct gggctctcgg acagtgtctg gagcgtcgca
     2341 ctggctcgct gtcttatctt tctgccgcta ctctggcttc tgccgggtta tggcttttgt
     2401 caggagctca tttcttttct ggtttgtacc aggcttttcc tcatctcctg ctcgttcatt
     2461 tgcccgttgt agcctgcata ggtccttgtc tgtattttta ctatcgcaag ttgatccacc
     2521 cttctattcc gattcgtccc agggacgtgg cccatttcgt tcccgcaatt ctggtagcgg
     2581 tggcgctcat tcctttttac tcgttgagtc cgggagaaaa gattgaaatc gcgaccggaa
     2641 tcaggaagga tctgcttgcg aagtggttcg gggttttgaa cgcgtttcca aagctttcgg
     2701 ccatcggcta cctttctgca tttgcgattc gcaatcgatt tctcctcagt cttcgcgcgc
     2761 cattgaatca tgtgcagctg tttgtcatcc tgtttggatg ctggattgtg tttggaacag
     2821 gtctcggttt gtttggaatg attttgcaga cgccttccct gcagcgttgg agtgcctgga
     2881 ctttgcctct gatcattttt gctctttatc ttgtgtccaa aagatatcct gagtttatca
     2941 gcgaactgca agtcgaagtg aagcgcgctc cttatggaaa gtcaacgctg accaggctcg
     3001 acgtggatcg cattatcgag aatctacaaa gctacatgaa gagtgaacgg ccttttctgg
     3061 atgaagatct caaactggca gacgtggcgg acgcgatgca cattacacct caccagcttt
     3121 ccgaaatttt gaacgaacgg cttggccgta attttgcgcg tttcgtcaat cagtacagga
     3181 ttgaagaggc aagaagactt ttgttggagg aatcagacag aactgttctt tcgattgcac
     3241 ttgctgttgg attcaattcc aaatctgcat tcaacggagc tttccttgat ttttgcggag
     3301 ttactccctc gcagtttaga tctggccaat caccgaagta gcgaatgata aaaccaaacc
     3361 tctacgcgta gttcggatag atcaatcagg acgacttcgg ttcttccgag tgttaaaatt
     3421 ttctccagga tgattcctgg aggggatatg aattacaaga atcttgcgga gatgtttgag
     3481 gcttcgtccg ttcgcttttc gaatcgaccg gcgtttgcct ggtatgacgc acacaatcaa
     3541 ctgcacgaaa aaagctatgg cgacctttat agagatgcaa cgtacctcgc tcgtgcgctc
     3601 gttgtgcttg gtgtcgagtc tcgtgaacat gtcggtctat taagtgacaa tcgatatgaa
     3661 tggatggtcg cagactgggc agtcatcttt tgcggtgcag cggacgttcc acgcggcgca
     3721 gacatcacgg actcggaaat cacttacatt ctgtcccatg cacagatcaa gattctattc
     3781 ctggaaaatg agaagctctt tgaaagaatt cagaatctga ggcattcgct tccattgctt
     3841 aagtccgtga tcgtaatgga tccgggcgcg cgcgaaaacc ctgagttcac gcgcatgcag
     3901 gatttgatta tacgtggtgc cgatttgctt ccagaatctg tggaagagcg agtggcacaa
     3961 attcggccgg aacatttgtt cactctcatt tatacgtcgg ggacaaccgg tgcacccaaa
     4021 ggagtgatgc tcacgcatgc aaatatgtgt tcgcagattt cgagaatgcc ctttgttccc
     4081 agaccagggg atcgcattct ctccatcttg cctgtgtggc atatatttga gcgcgttttt
     4141 gaaatgatcg ccattgcggg tggagcctgc acgtactaca cgaacgtgcg aaatttgcgc
     4201 gaagacttga agcgagtgaa gccatccttt atggcgtcag cgcctcgtct gtgggagagt
     4261 atttattccg gcattcagtc gaatgtgcag aaagcgtccg ggttcactcg cttcctattt
     4321 cgctctgcga tctttcttgc acgtgaagtc cgcggcggca tccgattctt gcgaggcggc
     4381 gagttggact tacgtggacg aaaccctttg atctcctttg tgcgggcgct gtggaccttg
     4441 tttaagatcg tatgtctcat gctgccgttt gctgttctcg atttcctggt tttgcgaaag
     4501 attcgcgcgg ctacaggagg tcacctgcgt ggttcagttt ctggcggagg agccttgccc
     4561 ttacacatag atacattctt caacgatatt gggattccgg ttttggaagg ttatggcatg
     4621 acagagactt cgcctgtgat tgcagttcgc acgtggaaat ctctggttcc agggaccgtt
     4681 gggcctttgt acccagacac acacattcgc attgtagatc ccgcgtccgg ggctcgcgtt
     4741 ttcgattcac agaatcctaa agtggggcgc ggcaagaaag gcgaaatcca tgtcagaggt
     4801 ccgcaggtga tggccggata tttcaagaat cccgaggcaa cggccggagt gctttccgat
     4861 ggctggctca acacgggaga tctgggcatg atgactttca acaattgcct gaagatcaca
     4921 ggacgttcca aagagacgat tgtgcttatg ggcggtgaga atgtggaacc cactcccatc
     4981 gaaagcaggc ttcttcaatc tgccttcatt gatcaggtta tggttgtagg tcaggatcga
     5041 aaaacactgg gagcgctcat cgtgcccaat cgatctgcct ttgacaacga ggaatacgag
     5101 agaatcgtcg caagcatgga agcccaggcc accattcgaa aagaagttca gtctttgatc
     5161 aatgctgagt ccggcttcaa gtcatttgag agagtggtgg tgttttcgct tgttccaggt
     5221 ccattcgtgg ttggagacga actaacagcg aagctttctc tgaaacgtca tcgcattgca
     5281 gagaagcatg cggaactcat tgagtctatg tattcatgag aaatcggagc gtcctcctga
     5341 cggggaagcc cgtcaggcga cattcaaatt aaagacccgc gcgtgcaatg gccaggaagc
     5401 tgtccgcttt cagggaagca cctccaatga gaccaccatc aatgtctttt tgagtcaaaa
     5461 gtgacttagc attgtccggc ttcatgcttc cgccgtagag gatacgcatt ccctctgcaa
     5521 cggatgcgtc atagagagac tgaatgagcg cacgaattcc tgcatgcact tcctgagcct
     5581 gttctggagt tgcatttcta cctgtgccaa tggcccagac cggttcataa gcgatcacgc
     5641 acttagttgc atcttcttta gtaattcctt cgaatgcttt tttcgtctgt gtggagacaa
     5701 catccatggt tctgtttccg tctctttccg cgagagtttc ccctacacag atcaccggaa
     5761 tgagtccgcc tttgagagct gtcttggtct tcttgtttac tgtatcatct gtttcgtgaa
     5821 aatacgtgcg ctgctctgag tggccgatga ttacatattc tgcgccggtg tcgcgaatca
     5881 tatcccagga aatcttcccg gtaaatgcgc cctgttcttc ccagtgcagg tcctgcgcgc
     5941 cgaccttgat ttgagtgcct tttctggctt cgcttgctgc gttgagtgca gtgtatggcg
     6001 ggcagaccag gagttccacg cctccaagat ctccggctcc ttgcgcaatt tccttgatca
     6061 gactttgcgc ctccccaatt ttcttgaaca tcttccagtt tcccgcgatc agtttctttc
     6121 tcatgccccg agcgtgggaa aatagacgcg ggagtaaagg aggttttttt tcagcagagt
     6181 gcaaaaaaga ctgaatggtc cagcaaaaca gtcgatagta ctatcaggcg gtatatacac
     6241 ggaagagcag ccgccttctg cacggaggca gaaatgttac acgaatacga agacatggat
     6301 gaaaactatc gttccgcaaa tttcctgggt cgcaaaagcc tcatcgcaac ggtgaagagc
     6361 gatacgttgc tacccgaaaa cggattgcgc gcaagatctc ttttgagcga agcacagttc
     6421 gcaagaatgg aagtgattcg ccagcgcact ctcaacaaag tctacgctcg ttttgaaagg
     6481 gactgaacag gcagccaccg tccgccaggg tccagcgtca aaaaagctta tattctttgc
     6541 tcagcatttc ggcgccttcc ggaatttccg gaagttcgtc gaggaattct cgcaaagaca
     6601 taatcttgct ttgaaatgcg ggcgggaaaa acggcttatg cttttgtggg accaccagga
     6661 tcactccgca atcccacatc tcagagagtt gcgtagacga gactccctgt tgtagagtga
     6721 acaaatgctt tcgcggaata cgatccgctt cgtttagaat ctggcgccat ctatccttgc
     6781 atgtggtctt taccccaagg aaacgcagag cagaagatgg aaaggctgga ttgtcgtagt
     6841 aatactttcc tggaaaagta aaatccggtc tcctcgatcc ctctgttgtt tcctgaaaag
     6901 aaaaacgaat tccgagaaat tgaaaaatcg cgcgaagatg gttctcaaga gaattgcccg
     6961 ctctggattt tcttctattt aagattgtgt gagatatctg gatcaaatct tccacggatt
     7021 caaaaggacg agccagcagg ctcccgtatt tttctctttc tatgattcga aaaattcgaa
     7081 attcgtagtc cagccactgc aataagagtg agtccaggtc caggcctttg ctttgcgcag
     7141 ttacggggat ttctgattta ggcggtgcca ggagcggtct gacgaattcg cgcgctttgg
     7201 agctgagcat ttctcctgat ggaaactctc ccccgaacgt tcgggcccac ggatgaatga
     7261 cttcttctaa agaggcatcg tcggatggtc tgcgaaattg aacggttcct gaatcagaag
     7321 gggaaaggcc aaaggatgcc agaaactcat cgatggactc atctgattgc aaaataaacc
     7381 catagtaatt tgtctcctgt ttcgccagaa ccagaagatc tcccacgtta tccggcgaaa
     7441 gaaattgaaa tccatttgta agtctgtact cggatcttgt ttttttgccg taccatacga
     7501 atctgctttt tgtggtccca tggtcccact gtacgtgaag ccaggtatct ctgttttctc
     7561 ccctttctcc tggctcatga aaaaacattt tccatgaatc gcgcggcatg tagaatcctg
     7621 cctggtggga accggtagct ccaacgtcgt tggcactgac gtacttgaag aagaatgctt
     7681 ccgcttccgc acacgatgca attgggtgag ccactagaga gccctcgaaa gactgcgcgc
     7741 aacagcagtg accaggggaa cggcaacgga gtttccgaat tgcttgtaag cctgcgtatc
     7801 tgaaaccgca atccgaaacg aatccggaaa gcccatcaat cgcgcgcatt ctcttggcgt
     7861 gagtcgacgt ggaacaggat tttgagatac cagaatttcc gctccgtctt tgtagtatcg
     7921 cgcgctgaga gttctggcta ctgagtctcc gtttacgagt ccatacccaa aaccatttcc
     7981 gtttttctta tgtttggcgg cgtaatcctg atgccatttc cacagatgcg cagataaagt
     8041 gtatttttga tgtaccgatt tttctagaat gcttttcagt tgcacgatct gctgcggtgg
     8101 atctggaaat gaaaagacag gagcaggata tacattggaa tcaaagccta cgagaaagat
     8161 tctttctcta tgctgtggaa cataaccctg cgcatcgagt actttatgaa acagagtata
     8221 gccgagttcc tcaaaggttt cgcgaatgat ctggaatgtg cgtccttgat cgtgcgaaag
     8281 caaattcttt acattctcca gtagaaatgc acgcggacgc ctggcttcca gaatctcggc
     8341 aatcttaaag aagagcgtgc cctgggttct gtctcgaaaa ccatgctctc tacccagact
     8401 tttctttttg gatactccgg cgatggagaa
//
LOCUS       DAORFF010000096         8340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4141_length_8340_cov_3.702112, whole genome shotgun sequence.
ACCESSION   DAORFF010000096 DAORFF010000000
VERSION     DAORFF010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4141_length_8340_cov_3.702112"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PKK76_05650"
     CDS             complement(<1..999)
                     /locus_tag="PKK76_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67986.1"
                     /translation="MIRDIFLRSSPGLALLFLLFFAECSGRDRKFQQIQDLIQAGNRQ
                     EAEDMIKAELLEENRKHAKEFDDGSLEAPLASPGARTAVWVKGESKLVILRNGKRNEV
                     SLGRRIKNIQLAPGGKFALILVIEGQGCKLAAVDLLEREVIHEISSCDPGAAITGDGQ
                     EILYSAQGGVGRIHLKNREKTVEDVLLEKSSFAAHYPKLTNRYYVVPDAQNHLYVFFG
                     SAGMYKMYLFVSGQNISMLKDGAARPQVYFADPEPELIKSEAKLPEREALGVIFTGSA
                     GKYRLASVFADRVSDETLPAPTIDPIQMLASGRIFYLNGETPALYHPVTGRKNLPMR"
     gene            1061..2887
                     /gene="aspS"
                     /locus_tag="PKK76_05655"
     CDS             1061..2887
                     /gene="aspS"
                     /locus_tag="PKK76_05655"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNL67987.1"
                     /translation="MQNWIHQVFTKRQPCGEPGDAGKKVYLAGWAFRYRDQGGLIFVD
                     LRDRSGIVQVVFDRSSMGDSFEQATHIRSEAVLAIEGKIRKRAPEAVNAKIPTGTIEV
                     VVEKFEVLNVSRPLPFQLDEFTEAGEEHRLKYRYLDMRREEMRDTLITRSKLNQCIRQ
                     YLEKDGFIEVETPILIKSTPEGARDFLVPARLSPGRFYALPQSPQLFKQILMVGGLEK
                     YFQIVKCFRDEDLRADRQPEFTQLDMEYSFFDEDMIVESLEGMWAHVLKKVFGVKVKT
                     PFPSITYHDSMEKYGNDRPDLRFGMELVDIADIAMKSSFQVFQKAVEAGGRVKALCVP
                     GGGVLSRKDIDDLTTWVGQDFGAKGLAWMKHEADGLKSAVSKFFTPELLSEISKRLKT
                     KEGDIVFFAGDRAHIVHNTLGNVRLRLAKQLNLIPEDEWNFVWVRDFPLFDRDVDTGA
                     LNSVHHPFTAPNPEDIPILKDPARFQKEGDKILSRAYDLVLNGVEIGGGSIRIHDKET
                     QAAVFTALGIGEEEARMKFGFLLEALEFGAPPHGGVAFGIDRVLMLLLKKSSIRDVIA
                     FPKTQKGTDLMSDCPSEVAVEQLRELKIKSLAEAKEQGAAAR"
     gene            2884..3861
                     /locus_tag="PKK76_05660"
     CDS             2884..3861
                     /locus_tag="PKK76_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNL67988.1"
                     /translation="MIERKISFDSRELFEHLCGIGDANIVSLERMLSVKLIPRGKNML
                     VHAPTEEIADNAQKYLEQVQERFSDPVRLRALEAGELLTILKAEAESRAPAGQANIST
                     DFKLFTTFRGKPIYPRTPRQAEFVESILSRPITISLGPAGTGKTFLSIVTACRLLTSG
                     EVDRIILTRPAVEAGESLGFLPGDMVQKVDPYLRPVYDALYDCFGADRTNELIALRKV
                     EIAPLAFMRGRTLSHSVIVLDEAQNCTLLQLKMFLTRLGKDSRMCLSGDASQIDLPPG
                     RSGLKRTMEILSGIDGIGVVEFGREDIVRHPMVTKIVAAFDRAQKEKDA"
     gene            3854..6184
                     /locus_tag="PKK76_05665"
     CDS             3854..6184
                     /locus_tag="PKK76_05665"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNL67989.1"
                     /translation="MPNLSGPLRLFLAKTYEILSRKRPAKFIRRFQAILISFTLVVVA
                     VVITYPIWNEASPDLRVGGAFDKDKVAPETVLARVDVEFPRLDLYEENKRAAEAAVPF
                     RFERNFSIIAGQEGKTLATMLADDVTALKACREKAKNEPAFLVCARAIPGFSRLRDVR
                     TLVYLPKIAAKGTEAAIALQEHLLILDKLPEDIQPEAAAVEVRDSGPGKPDTWRALPV
                     ESIVLRSQFSQEKTRNLMREVLAQAIPDPYMRSSYLDWILPYIATLEVARYMPEETAL
                     ARKEAVARLDIPHMRIAAGEPIVKQGDVIRQENRTALEVHARLHLREKAKRITAITLQ
                     HFLLLMILVFFAYRFESKRMSDMSANIIFFMNIWIFCGMLFAIETLWHEPNINPEDHI
                     FGSWVPIGIFAVLFSLLSGELFALATAVYLSLLVFGVSQYDPQSFLHSLVMSVMAVWA
                     GTRIRKRIHFLAASIFLMLLAGVLVTMGYLYLNRVIISQSGGDLLTTGYKSAMVKALL
                     MGAATAFTIVILPLYESVFNVPTRFRLQELSDPSTPLLQEFYRLAPSTWNHTLTVASL
                     TEKACERLGLNTLLARTGVYYHDIGKTRRAGFFVENQHLIPKPENIDKNNPEKAAKVI
                     IDHVLDGIEIARKIRLPREVMAFIPEHHGTSTMSFFYHQALEKSRRKVKRENFRYPGP
                     IPQSKETAIVMIADSVEAASRSLDEITETAVDSLIQKIINIKLAENQFDESGLTMGDL
                     AVIRKAFTEILLSSLHHRPKYPAMEATKKLEQARKG"
     gene            6184..6660
                     /gene="ybeY"
                     /locus_tag="PKK76_05670"
     CDS             6184..6660
                     /gene="ybeY"
                     /locus_tag="PKK76_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNL67990.1"
                     /translation="MHRIYRRVRIDVRDRIPSDRKIRTVLTQWLTHLGYKNAIIEISL
                     VSDPAIRRVNAEFRGKNKPTDVLSFPAQEFTRTRVPRLVSDWPQAGPLPLGDIMISIP
                     TARRQAREIGHSIQDEFYRLLSHGVLHLLGYDHERSRAEEKRMIAREDELTSFLWK"
     gene            6651..7421
                     /locus_tag="PKK76_05675"
     CDS             6651..7421
                     /locus_tag="PKK76_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNL67991.1"
                     /translation="MEIKGATVVITGAAGGLGKAMSFAFADAGAVVVISDLREDSLAA
                     AVNEVEARGGKAIAVTADTSKEADAERLMKEAVSRTGRLDVAVLNAGILRDGLLVRVD
                     KETGKVKGKLSLDQWQAVIDVNLTGVFLTGREAAVQMIETKSKGVIIPIASVSMHGNP
                     GQTNYSAAKAGVAAMTKLWAQELSRFRIRSAGIAPGFIATDMVMKDMKPDALAKWESV
                     IPIGRLGKPEEIAASALFIAQNDLVNGVILEISGGVKV"
     gene            7418..>8340
                     /locus_tag="PKK76_05680"
     CDS             7418..>8340
                     /locus_tag="PKK76_05680"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL67992.1"
                     /translation="MKPPGVFAALGFRNYRLWFAGQLGSLVGTWMQATAQAFLIYQLT
                     HSPAYLGYAGFASGAPAVLGLFGGVVSDRMPRRILLLITQTTMMILAAILAALVFAHL
                     VEPWHIIALSLGLGIANAFDAPARQSFVAELVDRDYLTNGIALNAIMFNLARAAGPAA
                     AGAVYGAFGAGFCFLVNAFSFLAVIVALLAIRTVASEKRKAESLRIEIAEGIGFAFGH
                     PVIRIILLIVAMVAIFGLGYITIIPAWTVTMLGGSALEIGLLQGAQGLGALLGAFAIA
                     AFARGSRGKMLLTGLLLFPLAILGFAMSRNV"
BASE COUNT         2031 a         2025 c         2177 g         2107 t
ORIGIN      
        1 tcgcatgggc agattcttcc ttcctgtgac agggtggtag agagcgggag tctcaccatt
       61 taagtaaaag attcttccgg aggcgagcat ttgaatggga tcaatggtgg gtgccggcag
      121 ggtttcatct gaaacgcgat ctgcgaagac ggaagccagg cgatacttgc ctgcactgcc
      181 tgtgaagatc actccaagcg cttcgcgttc tggaagtttc gcctctgatt tgatgagttc
      241 tggttcaggg tctgcgaagt atacctgggg tctggccgct ccgtccttta gcatggatat
      301 attctggccc gaaacgaata aatacatctt gtacattcca gcggatccaa agaatacata
      361 cagatgattc tgtgcgtctg gaaccacgta gtatcgattc gtgagtttgg gatagtgcgc
      421 agcgaaactt gacttttcca gaagcacatc ttccaccgtc ttctccctgt tctttaaatg
      481 aatgcgaccg actccgccct gcgctgagta caggatttcc tggccatctc ctgtgattgc
      541 agctcccgga tcgcacgatg agatttcgtg aatcacttca cgttccagca agtccaccgc
      601 ggcgagctta caaccctgac cttctatgac gagaatcagc gcaaattttc ctcccggtgc
      661 aagctgaatg tttttgattc ttctgcccag gctcacttca tttctttttc cgttgcgaag
      721 gatgacaagc ttggattctc ctttgaccca gaccgcagtg cgtgcgcctg gactggccag
      781 cggcgcttcg agagacccgt cgtcgaattc ctttgcgtgc ttgcgattct cttcgagcag
      841 ttctgctttg atcatgtctt ctgcttcctg gcgatttcct gcctggatga gatcctgaat
      901 ctgctggaac ttccgatcgc gacctgagca ctcagcgaaa aacaaaagga acagcagcgc
      961 caggccaggg gaagagcgaa gaaaaatgtc tcgaatcatg acgatgagac gaagtaaaac
     1021 aaattgacgc cctttacaag caggaatgtc tggaaaagcc gtgcagaact ggattcatca
     1081 agtttttaca aagagacaac cctgcggcga gcccggcgat gccgggaaaa aagtctatct
     1141 ggctggctgg gcgtttcgtt atcgcgatca aggtggcttg atctttgtcg atctgcgtga
     1201 tcgatcgggg attgttcagg tcgtgttcga ccgcagttcc atgggagatt cgtttgaaca
     1261 ggccactcac attcgttcgg aagccgttct tgctatagaa ggtaagattc gcaaacgcgc
     1321 gcctgaggca gtgaatgcga aaatccccac cggaaccata gaagtagttg tagaaaaatt
     1381 tgaagtgctc aatgtttcgc gcccgcttcc attccagctc gatgagttta cggaagcagg
     1441 agaagagcac agactcaagt atcgctatct ggacatgcgt cgcgaagaaa tgcgcgatac
     1501 tctgattaca aggtccaaac tcaatcagtg cattcgtcag tatctggaaa aagacggctt
     1561 cattgaagta gaaactccca ttctgatcaa gtctactccg gaaggagcca gggacttcct
     1621 cgttccagct cgtttatccc cagggcggtt ctatgcattg cctcaatctc ctcagctgtt
     1681 caaacagatt ctgatggttg gtggccttga gaaatacttt caaattgtaa aatgctttcg
     1741 cgatgaagac cttcgcgcgg acagacaacc agagttcact cagctggaca tggagtacag
     1801 tttcttcgac gaagacatga ttgtagaatc gctcgaagga atgtgggcac acgtgctcaa
     1861 gaaagtattc ggagtgaaag tgaagactcc gtttccttct attacatacc acgactccat
     1921 ggaaaaatac ggcaacgaca gaccggacct tagatttgga atggagctcg tagacatagc
     1981 tgacattgca atgaaaagct cgtttcaggt ttttcagaag gctgtagaag caggtggaag
     2041 agtcaaggct ctctgtgttc caggcggcgg agtgctttcg cgcaaggaca tagacgatct
     2101 aaccacctgg gtcggccaag attttggtgc aaagggcctt gcgtggatga agcacgaagc
     2161 ggatggactg aaatcagccg tctccaagtt ctttactcct gaacttcttt ctgagatttc
     2221 taagcgactc aagacgaaag aaggagacat tgtgtttttt gcgggagatc gcgcgcacat
     2281 tgtgcacaac actcttggca acgttcgact tcgcctggca aagcaattga atttgattcc
     2341 tgaagatgaa tggaatttcg tgtgggtgcg agactttcct ctctttgatc gggatgtgga
     2401 tacaggtgct ctcaattcag tgcaccatcc atttactgcg cccaaccccg aagacattcc
     2461 gattctaaaa gatccagcgc gttttcaaaa ggaaggagac aagattcttt cgcgcgccta
     2521 tgacctggtg ctcaacggag ttgagattgg aggcggatcg attcgtattc acgacaaaga
     2581 aacacaggcg gccgtcttca ctgctctggg aattggagaa gaagaagcca ggatgaagtt
     2641 tggctttcta ctcgaagcac ttgaatttgg cgcacctccg cacggcggag tcgcattcgg
     2701 aattgatcgc gtgctcatgc ttctgttaaa gaagtcatcg attcgcgatg tgattgcgtt
     2761 tccaaaaaca cagaaaggca cagacctcat gtccgattgt ccgtccgaag tcgcagtgga
     2821 gcagcttcgc gaattgaaga tcaagagtct cgcagaagca aaagagcagg gtgccgccgc
     2881 gcggtgattg aaagaaagat atccttcgac agtcgagagc tcttcgagca tctctgcggc
     2941 ataggggatg cgaacatcgt gtcgctggag cggatgcttt ctgttaagct gattccgcgc
     3001 ggaaagaaca tgctcgtgca cgctcccaca gaagagatag cggataacgc tcaaaaatat
     3061 ctggagcaag ttcaggaaag gttttcagat ccagtgcgcc tgcgcgcact cgaagcaggc
     3121 gagcttctta cgattctaaa ggcagaagca gaatcgcgcg ctcctgcagg tcaggcaaac
     3181 atcagcactg atttcaaact gttcactacc tttcgtggca aaccgattta tccgcgcacg
     3241 ccccgtcagg cggaatttgt ggaatctatt ttgtcgaggc ccatcacgat tagccttgga
     3301 cctgcaggaa ccgggaaaac ctttctgagc attgtcacag cttgcagact tttgacatcg
     3361 ggtgaagtgg atcgaatcat tctgaccagg ccagcggtgg aagcaggaga gtctctgggg
     3421 tttcttccag gagacatggt gcagaaggta gatccatact tgcgccccgt ttacgatgcg
     3481 ttatacgatt gttttggggc ggatcgcaca aacgaattga tcgctcttcg caaggtggag
     3541 atcgcgcctc tggcattcat gagaggtcgg accctgtccc acagtgtgat cgtgcttgac
     3601 gaagctcaaa actgcactct tttgcagctc aaaatgttcc tgactcgtct gggaaaggac
     3661 tccaggatgt gtttgtccgg ggatgcgtct caaattgacc tgcctccagg tcgatccggg
     3721 ttaaaaagaa ctatggaaat actttccggg atagacggaa ttggtgtagt tgagttcggt
     3781 agggaagata ttgtgcgaca tccgatggtc acaaagattg tagccgcctt tgaccgggcc
     3841 cagaaggaaa aggatgccta acctctcagg ccctcttcgc ctttttctgg caaagacgta
     3901 cgagatttta tctcgcaaga ggccggcaaa atttatccgc cgcttccagg cgattctcat
     3961 ttcatttacg cttgtcgttg tagcagttgt aatcacctat cccatctgga acgaggcttc
     4021 tcctgatctt cgcgtcggcg gtgcattcga taaggacaaa gtagcaccag aaacagttct
     4081 cgctcgagtg gacgttgagt ttccgcgtct ggatctttac gaggagaaca aacgtgcggc
     4141 ggaagcggcc gtgcctttca ggtttgaaag aaacttttcc atcatcgcgg gtcaggaagg
     4201 aaagactctg gccaccatgc ttgcggatga tgttaccgcg ctcaaagcct gtcgcgaaaa
     4261 agcaaagaat gagcctgcat tccttgtctg cgctcgtgcg attcctggat tttcccgttt
     4321 gcgcgatgta aggactcttg tgtatctccc gaagattgcc gcgaagggaa cagaagcagc
     4381 catagcacta caagaacatc ttttgattct ggacaagctg ccggaagaca ttcagcccga
     4441 agccgcggcg gtggaggtgc gggactctgg gccaggtaaa ccagacacct ggcgcgcact
     4501 tccagtggaa tcgattgttc ttcgcagtca gttttcgcag gaaaagacac gcaatctgat
     4561 gcgtgaagtt cttgctcagg caatccccga tccttatatg agatcgtcgt atctggactg
     4621 gattcttccc tacatcgcga ctctcgaagt tgcgcgttac atgccggagg agactgcact
     4681 tgcgcgcaag gaagcagtgg ctcgcctgga tattccgcac atgcgaattg cagcgggaga
     4741 accaattgta aagcaagggg atgtgatccg acaggaaaat agaaccgctc tcgaagttca
     4801 tgcaaggttg cacttgcgcg agaaagcgaa gcgcataaca gccattacac tacagcactt
     4861 tttgcttttg atgatacttg tcttcttcgc gtacagattt gaatccaaac gcatgagcga
     4921 catgtcagcg aacattatat tcttcatgaa tatctggatc ttctgtggaa tgctgtttgc
     4981 cattgagaca ctctggcatg agccaaatat caatccggaa gatcatatct ttggatcgtg
     5041 ggttcccatt ggaatatttg cagttttgtt ttctcttcta tccggagagc tctttgctct
     5101 ggcaactgca gtctaccttt cgcttctcgt atttggcgtt tctcaatacg atccgcagtc
     5161 cttccttcat tctcttgtga tgtctgtgat ggcagtatgg gccggcacac gcattcgaaa
     5221 acgcattcac tttcttgcgg cttccatctt cttgatgctg cttgccggcg tgcttgtaac
     5281 catggggtat ctgtatctca atcgagtgat catttctcag tccggcgggg atttgctgac
     5341 caccggatac aaaagtgcta tggtgaaggc acttttgatg ggtgcggcca ctgcctttac
     5401 aattgtgatt ttgcctctgt acgaatctgt tttcaatgtg cccactcgct tccgcttgca
     5461 ggagctttct gatccttcta ctcctctctt acaggagttc tatcgcctgg caccttctac
     5521 ctggaatcat actctgactg ttgcaagcct tacagaaaaa gcatgcgaac gacttggact
     5581 caacactctt ctcgcgcgaa caggcgttta ctaccatgac attggaaaaa ccaggcgagc
     5641 tggcttcttt gtagaaaatc agcatctcat tccaaagcct gaaaacattg ataagaacaa
     5701 tcctgaaaaa gcggccaagg taatcatcga tcatgtcctc gatggaattg aaattgcgcg
     5761 aaagatcagg cttccgcgcg aagtcatggc attcattcca gaacaccatg gcacttctac
     5821 catgtctttc ttctatcatc aggcacttga gaaatcaaga cgcaaagtaa agagagaaaa
     5881 cttccgctac cctggcccca tcccgcagag caaagaaact gccattgtga tgattgcaga
     5941 ttctgtggaa gcagcttcgc gctccctgga cgaaattaca gagaccgcgg tagattcttt
     6001 gatccagaag atcatcaata ttaagctcgc agaaaatcaa ttcgatgaat cagggctcac
     6061 gatgggagat cttgcagtga ttcgcaaagc attcactgag attttgctca gttcacttca
     6121 tcacaggcct aagtatccgg ccatggaagc cacaaagaaa ctggaacagg cgcgcaaggg
     6181 ctgatgcatc gaatctatcg tcgggttcgc atagacgtac gtgacaggat tccatctgac
     6241 cgcaaaattc gcacagttct aactcaatgg ctcacacacc tgggctacaa aaacgcaatc
     6301 attgagattt ctttagtatc tgatcctgcg atcagacgcg tgaacgcgga gtttcgcggg
     6361 aaaaacaaac caaccgatgt gcttagcttt ccggcccagg aatttactcg cactcgtgtg
     6421 ccgcgcctgg tatcggactg gccgcaggcg gggccacttc ctctgggcga tatcatgatt
     6481 tctattccca cggcccgcag acaggcgcgt gaaattggcc acagcatcca ggacgaattc
     6541 tatcgccttc tttcgcacgg agtactgcat cttcttggat acgatcatga aagatccagg
     6601 gcggaagaaa aaagaatgat cgccagagag gatgaactta cgtcgttctt atggaaataa
     6661 aaggcgcaac agttgtaatc acgggagcag caggcggact gggcaaggca atgtcttttg
     6721 cttttgcaga cgcaggagct gtagtcgtga tctctgattt gcgcgaagat agtctcgcgg
     6781 ctgctgtgaa tgaggtggaa gcgcgcgggg gcaaagcaat cgccgtgacg gcggacactt
     6841 cgaaagaagc ggacgcagaa aggctgatga aagaagcagt ttcgcgcaca ggcagactgg
     6901 atgtggcagt actcaatgct ggaatcttgc gcgatggcct tctggttcgt gtggataagg
     6961 agacaggcaa ggtcaaaggc aaactgagtc tcgatcaatg gcaggcagtc atcgacgtaa
     7021 atttaacggg cgtatttttg acaggaagag aagcggcagt gcagatgatt gagacaaaat
     7081 caaagggagt gatcatcccc atcgcctctg tttccatgca tggaaatcca gggcagacaa
     7141 actattctgc agccaaggca ggtgtggccg cgatgactaa actctgggcc caggaattga
     7201 gtcgatttcg cattcgttct gctggaattg cgcctggatt cattgcaacg gatatggtca
     7261 tgaaagatat gaaacccgac gcacttgcga aatgggaatc tgtcattcca atcggaaggc
     7321 tcggaaaacc ggaagaaatt gcggcttccg ctttgttcat cgcacagaac gatctagtaa
     7381 acggcgtcat actcgaaatc tccggcggcg tcaaagtgtg aaaccacccg gggtctttgc
     7441 agccctcgga tttcgtaact acagactctg gtttgcaggc cagctgggta gcctggtcgg
     7501 cacgtggatg caggcgactg cgcaggcatt tctgatttat caactcactc attctcctgc
     7561 gtatctgggt tacgctggat ttgcatccgg cgcgcctgca gtgctggggc tttttggcgg
     7621 agttgtatcc gatagaatgc cgaggcggat tttgcttctc atcacacaaa caactatgat
     7681 gattctcgcc gcaattctcg cggcgctcgt gttcgcacat ctggtagaac cctggcacat
     7741 catcgctctt tcccttggtc ttggaattgc caatgcattt gatgcacccg cgcgtcagtc
     7801 atttgtagca gagcttgtag atcgcgacta tctaacaaac ggaatcgcgc tcaatgccat
     7861 catgttcaat ctcgcgcggg ctgcgggacc tgcggcggcc ggtgcggtat atggtgcatt
     7921 cggtgcagga ttttgttttc ttgtgaatgc gttttctttt ctggcagtga tcgtggctct
     7981 tcttgcgatt cgaacagtcg ccagtgaaaa acggaaagca gaatcacttc gaattgaaat
     8041 tgcagaagga atcggatttg cttttggtca tccagtcatt cgcatcatct tgctgatcgt
     8101 ggccatggtt gctatttttg gtctgggtta catcacgatc attccagcgt ggactgtaac
     8161 gatgctcggc ggctctgctc tcgagatcgg gcttttgcag ggggcacagg gcctgggtgc
     8221 cctgcttgga gcgtttgcga ttgctgcttt tgcccgtgga tcacggggta agatgcttct
     8281 gaccgggctt ctgctttttc cactggcgat tctcgggttt gcaatgtcgc gtaacgttat
//
LOCUS       DAORFF010000097         8273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4203_length_8273_cov_3.511925, whole genome shotgun sequence.
ACCESSION   DAORFF010000097 DAORFF010000000
VERSION     DAORFF010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4203_length_8273_cov_3.511925"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1496
                     /locus_tag="PKK76_05685"
     CDS             108..1496
                     /locus_tag="PKK76_05685"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNL67993.1"
                     /translation="MKGVTSHCSKVFASTSAVLLVCTAASCMRLEPSVFDTSQNTEAL
                     AASLAIGWRVATPAFNPAAAYYSSAQTISITTATTGAKIYFTDDGSIPSSSATMYSTP
                     FHIWSAAGKTIQAIGTKSGMPDSSIASGVFGYSTLKTGQALCYDQVGTVISCAGTKQD
                     GELQLGVARSFTGPAAHAIYTSDYTTTDNATGLVWKSCSQGLSGAACATGTAQTLTWA
                     NADSDPTWGCNALNSANSGNGYAGIKTWRVPTRMEFETLVDHNASAAPWSFAAAFPAT
                     VSSMYWTSTAYQPSATKVWTLNFFTGFFGGNLKTNAYNVRCISGSPANPTYNYTDNGD
                     GTIKDNGSGLIWQKCSMGQNNDTTCSGAATTATLTPAMTYCNSLGLAGKSWRLPNIQE
                     LKTLIDTTTSASVKINGTAFPSTPAISNYHSSTTWNPAAGTSNWQIYLGDGIVDSSGK
                     TNAYNVRCVSGP"
     gene            1500..2465
                     /locus_tag="PKK76_05690"
     CDS             1500..2465
                     /locus_tag="PKK76_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL67994.1"
                     /translation="MIPQVVFRKACAVAILLIALSPARAEEILLKNGSTLSGRVTGQD
                     SMTVFVNTAQGMRSIPKAELVRIQYVSFTPEQKAQALDARRAKDAAAALEWERVRQLR
                     ETAERKRREDELEAKIRAEKEADARAASERAAALRELVKKGQMEKPADEPISYWDFAW
                     RSLVLPGWGHFYLDRPVFGAAYVAGTAGFVAAVYETHRRALSAVRENHREAQSNFILS
                     ATPVLPGFEMRMAYGLYSNAKAITVYQHKLDQYNGALAALATFYCVQVVHIIYNGIAW
                     ENGLLIVENRKDPGTIDAQFSAGPDYTEPGKKPGVAVVGALTMRF"
     gene            complement(2501..3217)
                     /locus_tag="PKK76_05695"
     CDS             complement(2501..3217)
                     /locus_tag="PKK76_05695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNL67995.1"
                     /translation="MVSTITLGDIVVQVTRKDIKNVHLSVYPPHGSVRIAAPRRMNLD
                     TIRLFAIAKLAWIKKQQRSLRAQNRETPREFLERESHYVWGKRYLLKIVEESGSPCIK
                     TKGKSLILRIKPGSSESRRDSALSDWYREQVREESALLLAKWEKRIGVKVKRVYVQHM
                     KTKWGSCNRQTGSIRLNTELAKKPPECLEYILVHELIHFLERNHGERFVALMNQHIPG
                     WRVYRNRLNRLPLRHEQWSY"
     gene            complement(3217..6270)
                     /locus_tag="PKK76_05700"
     CDS             complement(3217..6270)
                     /locus_tag="PKK76_05700"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HNL67996.1"
                     /translation="MTSLNTKERATQNRIIQLFKNSLGYAYLGNWEEREGNSNIEEEH
                     LARWLAKRGFQKEHISSAIYKLKTEAHNPNRPLVHNNMAVYSMLVYGVDVKLDATKNT
                     EQVPLIDWENPDQNDFAIAEEVSLHGAAERRPDLVLYINGIAVCVIELKRGSVNVTNG
                     IAQLLSNQKPEFNAWFFSTVQYVFAGNDSEGLRYGTIETEAKYFLTWKEDESENDLYK
                     LDKYLLKMCGKERLLDLIHNFVLFDGGKKKVPRVHQYFAIKAAQKNVRARTGGIIWHT
                     QGSGKSIVMILLSRWILANNPDARIAVITDRDELDKQMDRNFKNAGETIHKARSGRDL
                     LTQLAKPSPRLICSLIHKFGNKDAEDLDQLLKDLHAKPPKTSGEIFVFVDECHRTQSG
                     HLHKLMKAILPNAVFIGFTGTPLLKKDAATTLEVFGKYIHTYKFNEAVQDQVVLDLVY
                     EARDVDQRLTSVEKVDYWFEESSRRLKNKWQLDELKHTWGTMQKLLSSRSRMDKIIGD
                     IEFDFKTRPRLAGDRGNAILVASSIYEACKYYSLLQQTTFKNRCAVITSYNPQTKDIS
                     EEDMGANTETDKEFIYHTYTGILEDVVARPGKSKTETYEDQARNAFVDEPARMKLLVV
                     VDKLLTGFDAPSCSFLYIDKSMQDHGLFQAICRTNRLDGEDKQFGYIVDYKDLFKRVK
                     HALSVYTSELDHTAPGAEPEIMLKDRLATAREKLEDARQKYSILCEPVKPPRDYLDYQ
                     RYFCGNTEIPSDLEAREGYRMELYKSVAVLVRCYANLDTELEDAGYTKNQAEQIKQEV
                     KEAVALREKIRMAADEFLDLKPFEADMRRLIDNYIQADDPRPISPFGNLGLLDIIVKL
                     GIAEAISGHLGDFKGNKEAIAETIENNVRKKIIKEHLNDPNFYEKMSELLSEIIANRK
                     AKALDYEEYLKKIAKLIQTMQAGKTAEVPKTLDTKGKIALYNNLKQDEALALAIDAKV
                     RDVRPDDWRGVVPREQIIKKGLFEILGNEEEVNRIFQIIEQHTEY"
     gene            complement(6272..7198)
                     /locus_tag="PKK76_05705"
     CDS             complement(6272..7198)
                     /locus_tag="PKK76_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HNL67997.1"
                     /translation="MKNYYRVMAGKGSSLAEECFQQGFVGVDYGVAVDLTGKFPDDWR
                     QFNREFIPVYLQSHPEKTRIAAGSACGALWTLGRGMQKGDVVFVPDGEGSYHVGEVTS
                     DYQYQAGKNLPHRRQVRWYPEMVRRDATSPQMQGSTRSTGTIVDITGYANELEKLLSG
                     KGPAVLVSTDETVENPSAFALEKHLEDFLVANWKQTELGKNYDIYEEDGELTGQQYQT
                     DTGPIDILAISKDKTDLLVVELKKGRASDVVVGQTLRYMGYVQDELREPNQKVSGIII
                     ALEDDPKLKRALAMTPAIQFFRYEVSFKLRKA"
     gene            complement(7584..>8273)
                     /locus_tag="PKK76_05710"
     CDS             complement(7584..>8273)
                     /locus_tag="PKK76_05710"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNL67998.1"
                     /translation="PLPPFPEQKAIAEALGDVDALIESLEQLIAKKKQIKQGAMQELL
                     TGKRRLPGFSGEWEEKKLGDVCEITTGRKDVNQGNPNGIFPFFTCSRVHTFSDSYSFD
                     TEAILIAGNGDVGNLNYFAGKFEAYQRTYVLREFSVPVSYLWHHLSAFLAPALGLDKV
                     GSSIPYIKRENLIGFTYLSPRLAAESSAIATVLSEMDSEISAVEQKLSKTRAVKQGMM
                     QELLTGRIRLV"
BASE COUNT         1945 a         2144 c         2015 g         2169 t
ORIGIN      
        1 gcttcgcata gacgatgata caaaccccct gttcaggggg tgttctcgtt ctttaatctc
       61 tggctgtgca tgcagaacat aaacaagtgg acctccgggg aatcttgatg aaaggcgtta
      121 cttctcattg ttctaaagtt ttcgcttcaa caagtgcagt cttgcttgta tgtactgcgg
      181 cttcttgcat gcggcttgag ccgagtgtct ttgatacctc acagaatacg gaagcgctcg
      241 cagcaagcct cgcgatagga tggagggtcg ccactcctgc attcaaccct gccgcggcat
      301 attattccag tgcgcagacc atcagcataa cgactgcaac tactggcgcg aaaatctatt
      361 tcacggatga tggcagcatt ccatcatcca gtgcgaccat gtactccacg ccgtttcata
      421 tctggagtgc cgcgggaaaa acgatccagg caatcggcac aaaatcaggg atgcctgata
      481 gttctatagc cagcggtgtg tttggatatt ccacactgaa aacaggacag gcgctctgct
      541 atgatcaggt gggcactgtg atttcctgcg ccggcacaaa gcaggacggc gaattgcagc
      601 taggagttgc tcgaagtttt acaggaccgg cagcgcatgc gatttacact tcggattaca
      661 caactacgga caatgcaacc ggactcgtct ggaaatcctg ttcgcaaggc ttgagcggag
      721 ctgcatgcgc cacaggaaca gcgcaaacgt tgacatgggc gaatgcagac tcggatccta
      781 cgtggggatg caatgctctg aactctgcga actccggaaa cggatatgcc ggcatcaaaa
      841 catggagagt tcctacaaga atggaattcg aaacgttagt cgatcataac gcgtcggcgg
      901 cgccctggtc attcgccgct gcattcccgg cgactgtgtc gagcatgtat tggacttcca
      961 ctgcctacca gccgagcgca accaaggtat ggaccttgaa tttcttcacc ggattttttg
     1021 gcggcaacct gaagacgaat gcgtataacg ttcgttgtat atccggatct cctgccaatc
     1081 caacttacaa ttacacagac aatggagatg gcacaatcaa agacaatggc tcaggactga
     1141 tctggcagaa atgcagtatg gggcagaaca acgacacaac gtgttcggga gcggcaacca
     1201 cggccacact gacacctgcc atgacctatt gcaacagcct tggtcttgct ggaaaatcct
     1261 ggcgactgcc gaacattcag gaattgaaaa ctctcatcga taccactaca tcggcgagcg
     1321 taaagataaa tggcacggca tttccatcta ctcccgcgat tagtaattat cacagctcca
     1381 cgacctggaa cccggccgcg ggtactagca attggcagat ctatctgggt gacgggattg
     1441 tggattccag tggcaagaca aatgcgtata acgttcgatg cgtctcggga ccgtagcaaa
     1501 tgatcccgca agttgtgttt cggaaagcat gcgcagtcgc catcctgctc atcgcgcttt
     1561 cgcctgcccg cgcagaggaa attcttttga agaatggttc cactctgagc ggacgcgtga
     1621 ccggacagga ttcaatgaca gtgttcgtaa acacggcgca gggaatgcga agcatcccca
     1681 aagcagagct cgttaggatt cagtacgttt cttttacgcc ggaacaaaag gcacaggcac
     1741 tcgatgcgcg acgcgcgaag gacgcagcag cagccctgga atgggagcgc gtgcgacaac
     1801 tcagagaaac cgctgagcgc aaacgacgcg aagatgaact cgaagcaaaa atccgtgcag
     1861 agaaagaagc agacgcgcga gccgcctcag agcgcgctgc ggcacttcgc gaactcgtaa
     1921 aaaaaggaca gatggagaag cctgcagatg aacccatctc ttactgggat tttgcctggc
     1981 gatcgctcgt tctgcctgga tggggacact tctacctgga cagaccagta ttcggagcag
     2041 catacgtggc gggcacagcg ggattcgtcg cagctgtata cgagacgcat agaagagcgc
     2101 tcagtgcagt tcgcgaaaat catagagagg cgcagagcaa tttcattctg tctgcgacgc
     2161 ccgtccttcc tggcttcgaa atgaggatgg cgtacgggtt gtactcaaac gcaaaggcaa
     2221 tcacagtata ccagcacaaa ctcgatcagt acaacggagc gctcgcagcc ctggcgacgt
     2281 tctactgcgt gcaagtggtt cacatcatct acaacggcat cgcctgggag aatggtctgc
     2341 tgatagtaga gaatagaaaa gatccaggaa ccatcgacgc gcagttcagc gcaggacctg
     2401 attacacgga accagggaaa aaaccgggag tagctgttgt aggtgctttg acgatgcggt
     2461 tttgaaaggg gtggggtgtg cgatggcgaa cggttgtggt tcaatagctc cactgctcat
     2521 gtctcagcgg cagtcgattg agccgattgc gatacactct ccagccggga atgtgctgat
     2581 tcatcaaagc gacgaatcgt tccccatgat tccgttctag aaaatggatt agctcgtgca
     2641 caaggatgta ttccagacat tccggcggtt ttttggcaag ctctgtgttc agccgaatgg
     2701 acccggtttg gcgattgcag ctaccccact ttgtcttcat gtgctgcaca tagaccctct
     2761 tcactttaac cccaattctc ttttcccatt ttgcaagcag cagcgcagac tcttcgcgca
     2821 cctgctctcg gtaccagtcc gagagggcac tgtctctgcg cgattcggag gagccgggtt
     2881 tgattcgcag aattaaactt ttgccttttg ttttgataca tggacttcct gattcttcta
     2941 cgatttttaa gagataacgc tttccccaca catagtggct ttcgcgttcc aggaattcac
     3001 gcggagtttc ccgattttgc gcacgcaaac tacgctgttg cttcttgatc caggccagtt
     3061 tcgcgattgc aaaaagacgg attgtatcca gattcattct gcggggcgcc gctatgcgaa
     3121 cactcccgtg tggaggataa acgctcaaat gaacattctt gatgtccttg cgcgtcactt
     3181 gaaccacaat gtcgccgagt gtaattgtag ataccatcag tactctgtat gttgttctat
     3241 gatttgaaag atcctgttca cttcttcctc atttcccaga atttcaaaaa gacctttctt
     3301 gatgatctgt tcgcgaggaa ccactccgcg ccagtcatca ggacgtacgt cacgaacctt
     3361 cgcgtcaatt gcaagtgcga gcgcttcatc ctgcttgagg ttgttgtaaa gtgcgatctt
     3421 cccttttgta tccaaggttt ttggtacttc agccgtcttg cccgcctgca ttgtctgaat
     3481 cagcttcgcg attttcttga gatactcttc gtagtccagg gcttttgctt ttcgatttgc
     3541 aatgatttcg cttagaagct cagacatctt ctcatagaaa tttggatcat tgagatgttc
     3601 cttgatgatc ttctttcgca cattgttttc aatcgtttct gcgatagctt ccttattccc
     3661 tttgaaatcg cccagatgcc cggagattgc ctctgcaatc ccgagcttga cgatgatgtc
     3721 tagcaaaccg agattcccga aaggtgaaat aggtcgcgga tcatccgcct gaatgtagtt
     3781 gtctataagg cggcgcatgt ctgcttcgaa aggcttcaga tccaaaaact catctgctgc
     3841 cattcgaatc ttttcgcgaa gcgccactgc ttccttcact tcctgcttga tttgctccgc
     3901 ctgattctta gtgtaacctg cgtcctcaag ctccgtatcc agattcgcat aacagcgaac
     3961 aagaaccgca acgcttttgt acaattccat acgataacct tcgcgtgctt ccaggtcgga
     4021 aggtatttct gtgttcccgc aaaaatagcg ctggtagtcc aggtagtccc tgggcggttt
     4081 cacaggttcg caaagaattg aatatttctg ccgggcgtct tccaacttct cgcgtgcagt
     4141 ggcgagtctg tctttgagca tgatttcagg ctctgcacct ggcgcggtat gatccaattc
     4201 ggaagtatac acggaaagtg cgtgcttgac ccttttaaaa agatccttat aatccactat
     4261 atacccaaac tgcttatctt caccatccaa acgattcgtg cgacaaatcg cctggaacag
     4321 gccatgatcc tgcatggatt tatctatgta caaaaacgaa caggacggcg cgtcaaaacc
     4381 ggtcagcagt ttatccacta cgaccaggag cttcatgcgc gcaggttcat ctacgaatgc
     4441 attgcgagcc tgatcttcgt acgtttctgt tttcgacttg ccgggtctgg caacaacatc
     4501 ttccagaatt ccagtgtatg tgtgataaat gaattctttg tccgtctctg tgtttgcgcc
     4561 catgtcctct tctgaaatat ctttggtctg cggattgtag gatgtaatca cggcacatcg
     4621 attcttgaaa gtagtctgtt gcaggagaga gtagtacttg catgcttcat agatgctgga
     4681 agctacaaga atcgcattgc cacgatcgcc ggcaagacgt ggacgggttt tgaaatcaaa
     4741 ttcaatgtca ccgatgatct tgtccatgcg cgatctggaa ctgagcagtt tctgcatggt
     4801 gccccaggta tgcttcagtt catcgagctg ccatttgttc ttcaaacgcc tggaagattc
     4861 ttcaaaccag tagtccacct tttccacgga tgtcagcctc tggtctacgt cgcgcgcttc
     4921 ataaacaaga tcgagaacca cctgatcctg gactgcttcg ttgaatttgt aggtgtgaat
     4981 gtattttcca aagacctcga gagtagtggc agcgtctttc ttgagcagag gtgtacctgt
     5041 gaatccaatg aatactgcat tgggaagaat ggccttcatg agtttatgca agtgtccgct
     5101 ttgcgttctg tggcattcgt ctacgaacac aaagatttcg ccggatgtct ttggaggttt
     5161 tgcatggaga tcttttagaa gctgatccaa atcctctgca tccttgttgc cgaatttatg
     5221 aatcaaagaa caaatcaaac gcggacttgg ttttgccagt tgagtcaaga gatcgcggcc
     5281 gcttcgcgct ttgtgaatgg tctctcctgc attcttgaag tttctgtcca tttgtttgtc
     5341 cagctcatct ctgtccgtga tgactgcgat tcgcgcatct ggattgtttg caagaatcca
     5401 gcgagaaaga agaatcatca caatgctttt gccacttccc tgcgtgtgcc agatgattcc
     5461 gcccgtacgt gcgcgaacgt ttttctgtgc agccttgatg gcgaagtact gatgcacacg
     5521 cggcaccttt ttctttcctc catcaaagag cacgaagttg tggatgagat cgagcaacct
     5581 ctccttcccg cacatcttca acagatactt gtccagtttg taaagatcgt tttctgattc
     5641 gtcttctttc caggtgagga agtactttgc ctctgtttca attgtgccgt atctgagccc
     5701 ttctgaatcg ttgcccgcga agacatactg aactgtgcta aaaaaccacg cattgaactc
     5761 cggcttttga ttggatagaa gctgtgcgat tccattggta acattcacac ttccgcgctt
     5821 gagttcgatc acacagactg caattccatt gatgtagagc accaggtccg gtcgcctctc
     5881 tgcggctcca tgcaggctga cttcctctgc aattgcaaaa tcgttttgat ccggattctc
     5941 ccagtcaatg agcggtacct gctccgtatt ctttgtagca tccagtttca catccacgcc
     6001 gtagacgagc atgctgtaaa cagccatgtt gttgtgaacc agcggtcgat tgggattgtg
     6061 cgcttccgtt ttgagtttgt aaatagcaga gctgatgtgt tctttttgaa agcctctctt
     6121 tgcaagccag cgagctaaat gctcttcttc aatgttgcta ttgccttcgc gctcttccca
     6181 gttgcccagg tacgcgtagc ccagagaatt cttaaaaagc tgaatgatgc gattctgcgt
     6241 ggcgcgctct ttggtgttga gagaggtcat ttcacgcctt gcgcagcttg aagctcactt
     6301 catatcgaaa gaactgaatc gcaggcgtca ttgccagagc ccgtttcagt ttggggtcat
     6361 cttccagagc tatgataatg ccagagactt tttggttcgg ttcgcgcagc tcatcctgaa
     6421 catagcccat ataacgcaga gtctgcccta cgaccacgtc actggctctg ccctttttca
     6481 attcaacgac aagcaaatcc gttttgtcct tgctgatggc cagaatgtca atgggcccag
     6541 tgtctgtctg gtactgttgt cctgtaagtt ccccatcttc ttcgtaaata tcataattct
     6601 ttccaagctc tgtctgcttc cagttggcca cgagaaaatc ctccagatgc ttttcgagag
     6661 cgaaggcgga aggattctct acagtctcat ctgtagaaac caaaacagcg ggtccctttc
     6721 cggaaagaag tttctcgagt tcatttgcgt agccagtgat gtctacaatg gttcctgtgg
     6781 agcgagttga tccctgcatt tgcgggcttg tagcatcacg ccgcaccatc tctggatacc
     6841 atcgaacctg ccttctatgc gggagatttt ttcccgcctg atactgatag tcgctcgtga
     6901 cttcgcccac atgatagctg ccttccccgt ccggaacaaa gacaacatcg cccttttgca
     6961 tgcccctccc cagagtccag agagctccgc aggctgagcc ggcagcgatc ctggttttct
     7021 ccggatgact ctgcaagtag acaggaatga actcgcgatt gaactgccgc cagtcatctg
     7081 ggaatttacc ggtgagatcc acggccactc cgtaatctac tcccacaaat ccctgctgga
     7141 aacattcttc tgcaagagaa cttcctttcc cggccatcac tcgatagtaa ttcttcatag
     7201 ttgtctcttt gctccaggtg atcgcataac gttagatttt cgcgcgcata agaatattgg
     7261 gaaaggcgcc tcaggcgtct tttcctccac agtcagtcgt gaatttcagg attgaacttg
     7321 tcatagcagg cgaatcctgc cagtgagcaa ttcctgcatc atgaaccagg gaccggagcg
     7381 accggcccct ggaaccccat cgccgggggg tttcgactcc ccccggagcc ccggagcgag
     7441 caggggcaag ctcgcgtaac gtccacccct gcaccccgag tgatcgcata acgtcaaatt
     7501 tcgcgcgcgc gtaagaacat tggaaaaggc gccgcaggcg tcttttcctc cacagtcagc
     7561 cgtgagtttc aggattgaac ttgtcatacc agtctaatcc tgccagtcag caattcctgc
     7621 atcatgccct gtttgacggc acgcgttttg gaaagctttt gttccactgc tgagatttcc
     7681 gaatccattt ctgagagaac ggtggcgatg gcggatgatt cggcggctag acgtggagac
     7741 aaatacgtga aaccaatgag attttctctt tttatatatg gaattgagga gccaacctta
     7801 tcaaggccaa gcgcaggggc aaggaaggca ctgaggtggt gccacaagta tgagactggt
     7861 acactgaatt cgcgaagaac gtaagttcgt tggtaggcct caaacttccc ggcaaagtag
     7921 ttcaaattcc cgacatcgcc gttgcccgcg atcaagatag cctcagtgtc gaatgaatag
     7981 gaatcgctga acgtgtgcac tcgtgaacat gtgaaaaagg ggaaaatgcc atttggattt
     8041 ccttgattaa catccttgcg accggtagta atctcacata catcccccaa cttcttctct
     8101 tcccactctc cactgaaccc cggcaatcgc ctcttccccg tcagcaattc ctgcatggcg
     8161 ccctgcttga tctgcttctt ctttgcgatg agttgttcca gagattcaat gagcgcgtca
     8221 acgtcgccta acgcttctgc gattgccttt tgttctggaa agggcgggag ggg
//
LOCUS       DAORFF010000098         8267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4211_length_8267_cov_4.090964, whole genome shotgun sequence.
ACCESSION   DAORFF010000098 DAORFF010000000
VERSION     DAORFF010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4211_length_8267_cov_4.090964"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PKK76_05715"
     CDS             <1..974
                     /locus_tag="PKK76_05715"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL67999.1"
                     /translation="VFLLLGSLVFAFLISGRLSRPIESLASAAERIAGGELGVTVQIT
                     TAGEIGQLGATFNHMSRSLKDSERLKTEQAAIKRELEIAQGIQVAMIPEPQKVGPYSF
                     RGFMQTADEVGGDYVDVIHIDGRSPRFWFFIGDVSGHGLRAGLTMLMAQTAIHSTMEV
                     SPDLEPSQAFMHVNDVLFANIRRLRERKYMTAAFYRADAKGNFSFAGLHQDALIYRAA
                     GKKLEVVPTDGFWLGVDAGVKKDMRAGKFKLSVGDLLFLFTDGLVESMNGKKEMFGQE
                     KIEEIVRKHGTSDLAGLEERIMNSLRAHTGGLRPADDITFAMIRREK"
     gene            976..1443
                     /locus_tag="PKK76_05720"
     CDS             976..1443
                     /locus_tag="PKK76_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68000.1"
                     /translation="MSFSLTIEPEWNVIKKIKESLQTDPALKGLSKDFLEQTTVTAIE
                     LLENALKYTDSDSGRPVEFSFDSQAGYCAFRVKNYSKNAQNKDALRSVIDRIRDGDPF
                     ELYVARLEQLKEDPDGHSRMGLYRIAYEGEYHLSADIDGESVTILARRPLENA"
     gene            1443..1811
                     /locus_tag="PKK76_05725"
     CDS             1443..1811
                     /locus_tag="PKK76_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68001.1"
                     /translation="MALSNLTMQDFEIEAVVSGSNIELNWKGAMHAPNPEDVLDPYFE
                     QVIAEALKAKLGVKCNFVPLEYMNSASIPPLIALLRTLADKKIQGEFVYDSNRKVQSA
                     SFRALDVIARKSKYTSVRGI"
     gene            1811..3016
                     /locus_tag="PKK76_05730"
     CDS             1811..3016
                     /locus_tag="PKK76_05730"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL68002.1"
                     /translation="MVDVRDLWQESPSAELVLRDGKLMAMNPAAEKLLGITASDLGDS
                     VWEERVFDEKARKKFRSFLESQAPVSSGLDASGADGSIRHIRFLKRNIPSHGTLLSVQ
                     DVTETREDAEAFQAGYDEFIRVTTDLEDALATIEKQNRQLERQKQILEGELQVAHAVQ
                     AQILSQDFEKHTLVNVFGTYQAMTELGGDMWEFFESENGTWVVIADVMGHGVASSLIS
                     IAAKSIFKRRFEEASSGKMDLAELVKAANRDLCELTHSNYFLTACAVHIDSNHRLEYL
                     TAGHPPVLYVPADSSRQVELLFTEQPMLGVFRNIEYHSVPFQMFEGDRIFIYTDCLIE
                     SVNAEGDVIDLLQIADRLRLKPGTTPQTVVQGMLEYRREFAGSDRLPDDLTMVCIEIP
                     QAAGLRVAK"
     gene            3013..4527
                     /locus_tag="PKK76_05735"
     CDS             3013..4527
                     /locus_tag="PKK76_05735"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNL68003.1"
                     /translation="MIRVYACILTAAMLCAAGLIAERRHIAVIVSSDTGIYADALTGF
                     YSSFDPGLAQIDQPVYVDALPDPARFFSERSDADLIVTIGSKATQLALAEGHGSKIVF
                     SMVSSPRAVGVASRPDVCGTSMDVSLSEFFKSLRELSPSARNVYAFYSTPEGEFAASE
                     GEYLDLQLGVEYHKRRLSGKSELPRALESIQGKADAFYLVPDALYDSEQFQIVSDFCK
                     KNKIVLMTSFSSLMQAGATFSLRPDYTRIGRVTAEMAGTVLQGKGCGPRRVVFPDQAY
                     FSLNERYAEESGLTIPQSVKETASRTRLFLSAVNLMNEGKPKSALIVFDAILKKAPDD
                     AAAKRYRDAIVERQSGAQTRQMMESARQYLAAKNFERAIQEYKNVLSVNAGNQEAMAG
                     LDEAKLGISEQYRARGDAAVDPYDKARFYSSAVQYMPNNTRAAMALDGVRARQKQRLA
                     EDLKLGIADYNAREFDRAVKTFENILLIEPGNREAQEYLRIAVRKRDALRKLQE"
     gene            complement(4537..7248)
                     /locus_tag="PKK76_05740"
     CDS             complement(4537..7248)
                     /locus_tag="PKK76_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNL68004.1"
                     /translation="MGVRWGLAAWIVLFAGALSARETRIGAIQPFQSPEEKGVESGVR
                     ETLRRSLQNLEIQAEDTGENRPDGKGDYFATIYYSRGAAGALNLYGQIYDSAESTLID
                     AVSITDESIRGAGIALPAEETRGSDEETISRFANRMAVRIKGNPRKVERTENIQESLL
                     SLEIGKKIQFPIRRSDTSGEVFRMLEDQRIVTATRQATRVKEAPAAVHVITEEQIRQR
                     GYRTLTDALHDAPGFDIIHVYGIFPDLIHQRGLVGNNQRTLVYVDGILDNNLTENGVL
                     GGTLRFPLYNVERIEVIGGPASALYGANAFNGVINIITKDGTTPGGGVQALGGWSEDN
                     FHNPGGGGSISARGTTGDADLRFSYSAAATYLKSAGPYFGDIQHLDKRNANANDAAYY
                     TETQFCGGVCNPDAKSIGYWWSKYYDSPEDTYNITARFSAGGFRFETINWQYLQGNGT
                     FSNGTQQIDTDRIKGFRGASWDFRNNSASLGYLYAISSKVSLDSEITARHTDVLSSSH
                     DEAPNSPGPYAYYRPYDTTPSSNYERPDYAYVANERLLWEQTRGSNLTVGVESSFTVV
                     PTAYGSARRFRYVNNAAYLQQIWGPWEWLSLTAGYRYDYSTIYGAANTPRIGTVITPM
                     KDLSIKLLASSGFRAPTAWELFNATNQRKANPDLKPERLRSYEFGVGYRFFRRYYVSV
                     QQYYNRINNLLLEVQTAEPNPNQSGTNWNQNQNVGRATIYGTEVETDVQISNSLGLFL
                     NYTYNHGKYEDLSYSLTSSPSTRGRTGDDPLFDATAAAVKQTLGRSIVPESGSIPNIA
                     PHHANAGLTWKILKNVTAFFGINYVDIRRTVATNPEKTVPGYTMGTLNLRWEDAFLSG
                     LDFSLLVRNAGNQQFFDPGIRAATGSYYPTEHPLEKRNIWLTAGYRF"
     gene            complement(7290..>8267)
                     /locus_tag="PKK76_05745"
     CDS             complement(7290..>8267)
                     /locus_tag="PKK76_05745"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNL68005.1"
                     /translation="AVVTDGSAVLGLGNIGPEAAIPVMEGKALLFKQLADIDAWPVCL
                     DTQDTDEIVETVRRIAPVFGGINLEDISAPRCFEVERRLQDLGIPVFHDDQHGTAIVL
                     LAGLLNACRITGKQISDLRIVINGAGAAGIAITNILRDDGPGDKIIAKDIIVCDTQGA
                     IHAGRTDLNSEKQELLRYTNFENRSGSIQEVMKGADVFIGVSKANLLTADHVRTMAKD
                     SMIFALANPVPEIMPAEAKLGGAAIIGTGRSDFPNQVNNALAFPGIFRGALDAGARKI
                     TDEMKIAAARALAEMVKNPTPEHILPRVLDPAVSATVAKAVYKAAHDQA"
BASE COUNT         1926 a         1979 c         2231 g         2131 t
ORIGIN      
        1 ctgtcttctt gcttctgggt tcacttgtat ttgcatttct catttcagga aggttatcga
       61 gacccatcga atcgcttgca tccgcggcgg agaggatcgc gggcggggaa ctcggcgtta
      121 cagttcagat cacgactgca ggagaaatcg gacagctggg cgcaaccttc aatcacatgt
      181 cgcgcagttt gaaggattcg gagagactca agacggagca ggcagccatc aagcgagagc
      241 ttgagattgc tcagggcatt caagtggcca tgattccaga gccgcaaaag gtcggccctt
      301 attcgtttcg aggcttcatg cagactgcag atgaagtggg cggagattat gtggatgtca
      361 tacacataga cgggcgctcg cccagattct ggtttttcat cggagatgtt tcaggtcacg
      421 gtcttcgcgc aggtctaacc atgctcatgg cgcagactgc cattcattct actatggaag
      481 tttcgccaga ccttgagccc tcgcaggcat tcatgcatgt gaacgacgtt ctattcgcca
      541 atattcgacg attgagggaa aggaaataca tgactgccgc gttttatcgc gcggatgcaa
      601 aagggaattt ctcttttgcc ggtctccatc aggatgcatt gatctacagg gccgccggca
      661 agaagctgga agtggttccg acagatggtt tctggctggg ggtggacgct ggcgtcaaaa
      721 aggacatgcg cgcgggcaag ttcaagctgt ctgtggggga tctccttttc ttgtttacgg
      781 atggcctcgt agaatctatg aatgggaaaa aagaaatgtt cggtcaggaa aaaatagagg
      841 agatcgtgcg taagcacgga acctcggatc tggccggcct ggaagagcga atcatgaaca
      901 gcttgcgtgc gcatacaggg gggctcaggc cagccgacga tatcactttc gccatgattc
      961 ggcgggagaa gtaatatgtc attttcgctt acaattgaac cagaatggaa cgtgatcaag
     1021 aagatcaaag aatctctgca aacggatcct gctctcaaag gactcagcaa agatttcctc
     1081 gagcagacca cagtcaccgc cattgagttg cttgagaacg ctttgaaata caccgactct
     1141 gattcaggac gacccgttga attctcattt gattcgcagg ccggatattg tgcctttcgc
     1201 gtgaagaact atagtaagaa cgcccagaac aaagacgctc ttcgctcggt cattgatcgt
     1261 atccgcgacg gagatccttt tgaattgtat gtagcacgat tggaacagtt gaaggaagat
     1321 cccgacgggc acagccggat gggattgtat cgcatagcgt acgaaggaga gtaccatctc
     1381 agtgctgata ttgacggcga aagcgtgacg attctggccc gccggccgct ggagaatgca
     1441 taatggctct ttcgaatttg accatgcagg acttcgaaat cgaagcagtc gtcagcggtt
     1501 cgaatatcga attgaactgg aaaggtgcca tgcatgcccc caatccagaa gatgtgctgg
     1561 atccttattt cgagcaggtg atcgcagagg cacttaaagc aaagcttgga gtgaagtgca
     1621 acttcgtgcc tctggaatat atgaattcag caagtattcc tcctctgatt gctcttcttc
     1681 gcactctggc agacaaaaag attcagggtg agttcgtgta cgattccaat agaaaagttc
     1741 agtcagcttc ctttcgtgcc cttgatgtga ttgcgcgaaa gtcaaagtac acatctgttc
     1801 gagggatttg atggtagacg tccgggattt atggcaggag tcgccttccg cggaactggt
     1861 tctgcgagac ggtaaactaa tggccatgaa cccggcggca gagaagctgc tgggaatcac
     1921 tgcttccgat ctgggtgaca gcgtctggga agaaagagtc ttcgatgaaa aagcaagaaa
     1981 gaagtttcgc agctttcttg aaagccaggc tcccgtatcc agcggccttg atgcaagcgg
     2041 cgcggacggt tcgattcgtc atattcgctt tttaaaaaga aacattcctt cgcacggaac
     2101 tttgctcagc gttcaggacg tcactgaaac gcgggaagat gcagaagcgt tccaggcggg
     2161 ctacgacgag ttcatccgcg tcacaacaga tctagaagac gcgcttgcga cgattgaaaa
     2221 gcagaacaga cagctcgaaa gacaaaagca gattctagaa ggagagctcc aggttgcaca
     2281 cgcagtgcag gcgcaaatcc tttcgcagga cttcgagaag catactcttg taaacgtttt
     2341 cggaacctac caggcaatga cagagcttgg cggggacatg tgggaatttt ttgagtcaga
     2401 gaacggcacg tgggtggtga ttgctgatgt gatgggacac ggagttgctt ccagtctcat
     2461 ctccattgct gcgaagtcaa ttttcaaaag aagattcgag gaagccagtt ctggcaaaat
     2521 ggatctggca gagcttgtga aagctgcgaa tcgcgatttg tgcgaactca ctcattccaa
     2581 ttactttctg actgcatgcg ccgttcatat tgattcaaat catcgtctgg aatacttaac
     2641 tgcggggcat cctccggttc tgtacgttcc cgccgattcg tccagacagg tggaacttct
     2701 ttttacagag cagcccatgc tgggtgtgtt tcgcaacatc gaatatcact cagtgccatt
     2761 tcaaatgttt gaaggagatc gaatcttcat ctacacggat tgtctcattg aaagcgtcaa
     2821 tgcagaagga gacgtgatcg atttgcttca gatcgcagac aggcttcgtt tgaaaccggg
     2881 cactactcct cagacagtag tgcagggtat gctggaatat cgccgcgaat ttgcagggtc
     2941 agatcgcctt ccagacgatt tgaccatggt gtgcattgag attcctcagg cggccgggct
     3001 gcgggtggca aaatgattcg cgtctatgca tgcattctaa ctgccgcgat gttgtgtgcg
     3061 gccgggttga ttgcggagcg caggcacatt gcagtcatcg tatcttctga cactggcatt
     3121 tatgcagatg cactgacagg cttttattct tcgtttgatc cggggcttgc acaaattgat
     3181 caaccagtgt atgtggatgc ccttccagat ccagcgcggt tcttttcaga gagatcagat
     3241 gcagatctca ttgtgaccat cggatccaag gcaacgcagc ttgcgcttgc ggaaggccac
     3301 ggaagcaaga tcgtcttttc catggtatcg agtccgcgcg cggttggtgt tgcttccagg
     3361 cccgacgtgt gcggcacaag tatggacgta tccctttctg aattcttcaa gtctcttcgc
     3421 gagctttcgc cttccgcacg taacgtgtat gcattttatt ccacgcctga aggcgaattt
     3481 gcagcctctg aaggggagta tctggatttg cagttgggcg ttgaatatca caagcgcaga
     3541 ctttctggca aatcggagct tccgcgagct ctcgaaagta tccaggggaa agcggacgct
     3601 ttttatctag tgccagatgc cctttatgac agcgaacaat ttcagattgt ttctgacttc
     3661 tgcaagaaga acaagattgt gctgatgact tcgttctcat ctcttatgca agcaggtgcg
     3721 acgttctcat tgcgtccaga ctacactcgc atcgggcgtg tgacggcaga gatggcaggt
     3781 actgttcttc aggggaaagg atgtgggccg cgcagagtcg ttttcccgga tcaggcgtat
     3841 ttctcgttaa acgagcgtta tgcggaagag tccggtctga cgattccgca atcagtcaaa
     3901 gagactgcca gtcgcactag attgtttcta tcagccgtca acttgatgaa tgagggtaaa
     3961 ccaaagtcgg cactgattgt ttttgatgcc atcttgaaga aagcgcccga tgacgcggcc
     4021 gcgaaacgtt acagggacgc cattgtagaa aggcagagcg gcgcgcaaac caggcaaatg
     4081 atggaatccg cgcgccagta cctggccgca aagaattttg aaagagcgat ccaggaatat
     4141 aaaaacgttc tcagcgtgaa tgctggaaat caagaagcga tggccggcct ggacgaagcg
     4201 aaactcggaa tcagtgaaca gtaccgcgct cgcggagacg cggcagtcga tccgtatgac
     4261 aaagccaggt tttacagttc tgcagtacaa tacatgccaa acaacactcg tgctgccatg
     4321 gcactggacg gagttcgcgc caggcagaag cagcgcctgg cagaggacct caaactgggg
     4381 attgcggatt acaatgcgcg agagtttgac cgggcagtga agacttttga gaatattcta
     4441 ttgatcgaac cagggaaccg agaagctcag gaataccttc gcatagcagt gcgtaagcga
     4501 gacgctctga gaaaacttca agagtaagag ttctctttag aatctgtatc cggcagtgag
     4561 ccagatgttt cttttttcta gcggatgctc tgtgggatag taggagccag tggctgcccg
     4621 gattccagga tcaaagaact gttgattgcc tgcgtttcgc accagaaggg agaaatcgag
     4681 gccagaaaga aacgcatctt cccatctgag gttcaaggtt cccatagtat acccgggcac
     4741 agtcttctct ggattggttg ccacagtgcg acgaatgtcc acatagttga tgccaaagaa
     4801 cgcggtgacg ttcttcaaga ttttccaggt aagtcctgca tttgcatggt gaggcgctat
     4861 gtttggaatg gaaccggact ctggcacaat ggatctaccg agagtctgct tcacggcggc
     4921 ggctgtggcg tcgaagagcg gatcgtctcc tgttctacct cttgtggagg gagaggaagt
     4981 gagtgagtaa gacagatctt cgtatttgcc gtggttgtaa gtgtagttca agaacagtcc
     5041 aagagagttc gagatttgca cgtcggtttc tacttctgtt ccgtaaatcg tggcgcgtcc
     5101 tacattctgg ttttgattcc agtttgtgcc gctctggttt ggatttggct ctgccgtttg
     5161 cacttcgagg agtagattgt tgattctgtt gtagtattgc tgaacggata cgtagtaacg
     5221 ccgaaagaaa cgatagccga ctccaaactc atacgatcgc agcctttccg gttttagatc
     5281 ggggtttgct tttctttgat tcgttgcatt gaatagttcc caggctgtgg gcgcgcgaaa
     5341 tccgctggaa gcaagcagtt tgatggacag gtccttcatt ggagtgatta cagttccaat
     5401 gcgcggagtg tttgccgcgc cgtagatggt gctgtagtca tagcggtatc ctgctgtcag
     5461 agacagccat tcccagggtc cccagatttg ctggagatag gcggcattgt tgacgtatct
     5521 aaatcttcgc gcggaaccat acgcagtggg cacgactgta aaagaagatt caacgcctac
     5581 agtgagattg gatccgcgag tttgttccca gagcagcctt tcgtttgcaa cgtatgcata
     5641 gtccggtctt tcgtaattgg aggaaggtgt agtatcgtac ggtctatagt aggcgtatgg
     5701 gcccggactg ttgggcgctt cgtcgtgact ggagctaagc acgtctgtat gtctggctgt
     5761 gatctcactg tcgagggata ctttagagct gatcgcgtag aggtatccca ggcttgcgct
     5821 gttgtttcga aaatcccagg acgcgccgcg aaaccctttg attcgatccg tgtctatttg
     5881 ttgcgttccg ttggaaaatg tgccgtttcc ctgcagatat tgccagttga ttgtctcgaa
     5941 tctgaagcca ccggcagaaa atcgcgccgt gatgttgtat gtgtcttcag gagaatcata
     6001 gtatttagac caccagtatc caatcgattt agcatccgga ttacacactc caccacagaa
     6061 ttgcgtttca gtgtagtagg ctgcgtcatt tgcgttcgca tttcttttgt ccaggtgctg
     6121 gatgtctccg aagtaagggc ccgcagattt taaatacgtg gcagctgcgc tgtaactgaa
     6181 tcgtaagtcc gcatctcctg tggttccacg tgcgctgatg gatcctccgc ctccagggtt
     6241 gtgaaagttg tcttcgctcc atcctccgag tgcctggact cctccacccg gcgtggttcc
     6301 gtctttggtg atgatgttga tgacaccgtt gaatgcgttg gctccgtaga gtgcggatgc
     6361 aggtccgccg atgacttcaa ttctttctac attgtagaga gggaatcgca gagttccgcc
     6421 aagcacaccg ttttccgtca ggttgttgtc gagaattcca tccacataga ccagagttct
     6481 ctgattgttt cccacaaggc ctctctggtg aattagatcc ggaaagatgc cgtaaacatg
     6541 aatgatatca aaaccaggcg cgtcatgcaa tgcgtctgtg agagtacggt accccctctg
     6601 cctgatttgc tcttctgtga ttacgtgaac tgccgccggt gcttctttga ctctggtcgc
     6661 ctgacgggtt gctgtcacga ttctctggtc ttcgagcatg cgaaagactt ctccagatgt
     6721 gtctgacctg cgaatcggga actgaatttt ttttcctatt tcgagagata gaagagattc
     6781 ctggatgttt tctgttcttt ctacctttcg cgggttgcct ttgatgcgca ctgccatgcg
     6841 atttgcaaag cgcgaaattg tctcttcatc tgaaccgcgt gtttcttctg ctggtagagc
     6901 gatgcccgcg ccacggatcg attcatctgt gatgctgaca gcgtcaatca gagtgctctc
     6961 tgcggaatca taaatctggc catagagatt gagtgctcca gcagcacctc tggagtaata
     7021 gatagtggcg aaatagtcgc ctttgccgtc tggtctattt tcgcctgtgt cctctgcctg
     7081 gatctccaga ttttgcagag atctacgcag tgtttcgcgc acgccgctct ctactccttt
     7141 ttcttctgga gactggaatg gctgaatggc gccgattctg gtttcgcgcg cgcttaaagc
     7201 cccggcaaac aagacgatcc atgctgccag cccccatcgc acgcccattt ctacttcgcg
     7261 tacggcgaag ggtcaaatca aaaaaaacct caggcctggt cgtgcgcagc tttgtagact
     7321 gcttttgcaa ccgtagcgga taccgcagga tccagaactc ggggcagtat gtgctcgggc
     7381 gtcgggtttt ttaccatttc tgccagagcg cgtgctgcgg cgattttcat ttcatctgtg
     7441 atttttcgcg cacccgcatc cagcgctccg cgaaagatgc caggaaaagc aagagcattg
     7501 ttgacctggt ttgggaagtc acttctaccc gtaccaatga tcgctgctcc gccgagttta
     7561 gcctctgcag gcatgatctc agggacaggg ttggccagag caaagatcat ggagtctttt
     7621 gccatggtgc gtacatggtc cgcagtcagt agatttgctt tgctcacgcc aatgaacacg
     7681 tctgcaccct tcatcacttc ttgaatcgat ccgctacgat tttcaaagtt cgtataacgt
     7741 aacaattcct gcttttcaga attcagatcc gtgcgcccgg catgaatggc tccctgagta
     7801 tcgcacacga tgatgtcttt tgcgatgatc ttgtctccgg gaccatcatc gcgcagtatg
     7861 ttcgtgatgg cgatgcctgc cgctcccgca ccattgatca cgatgcgaag atccgaaatt
     7921 tgttttcctg tgattctgca ggcgttgaga agtccggcca gtagcacaat ggcagttccg
     7981 tgttgatcgt catggaaaac cggaatgccc agatcctgaa gccttcgctc cacttcaaaa
     8041 caacgcggcg cagatatgtc ttcgaggttg atgccgccaa atacaggtgc aatgcgacga
     8101 actgtttcta caatttcgtc tgtgtcctgc gtatcaagac acacaggcca tgcgtcgatg
     8161 tctgccagtt gcttgaagag tagagctttt ccctccatta caggaatggc tgcctccggt
     8221 ccaatgtttc cgaggccaag aactgcagat ccgtctgtaa ccacagc
//
LOCUS       DAORFF010000099         8216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4250_length_8216_cov_3.434506, whole genome shotgun sequence.
ACCESSION   DAORFF010000099 DAORFF010000000
VERSION     DAORFF010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4250_length_8216_cov_3.434506"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1886
                     /locus_tag="PKK76_05750"
     CDS             <1..1886
                     /locus_tag="PKK76_05750"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNL68006.1"
                     /translation="IDGSKQSYGATVAGYTNQGYALSGLIVVMLNNRNNAGDAVAHVT
                     TTSAAGTFTFTAQHVVLPAGLTDDSARVAYAASQFAGGNVTTANISGTDSVSDSITTD
                     GTNYWFKSNSYSIYIIDPRGHMSGSLTTATNGSVASNTPTAATTLSVGAIAAHLPRGK
                     ISGVVTDAFSTEAISGALVQLGRDTNPDPSLISFSPARKDSNPIANVSRLSNSADVQV
                     ADVTTPSNGSYSFDNIDPGDYVLQISKNGYVTGTIPVTVPSNGGSVSVNPPIVVDGPK
                     GNLTGYVKLPGGFNFTDPYSLEVTHPASGIHPPAGVIPASLASGPSQFANAPQYSIFA
                     LPAGVWKLKFVSAGYKTVEGIVTIQGNATTNFDIITMVPGSHPPASIGGRALNALNNQ
                     PISSLTVRLRPGVGVTTGAYAIDANGATLPAVVTGSNGAFVVANVPAGNYTLEVSGSG
                     YSTTYRTVISAGTDTPAAQDILVSPVLGANEVRIVLSWNATPKDLDSHLEYGSGWPNQ
                     VVWNDRTKLSGDLLLDWDVVTGYGPETVTMKGTVWTQPRRGYSVYNWSRGKNRDTVLT
                     IGQSGATVKVFKSQGLVRSVAAGPSQVSDWWQMFCFSSAQGLIDLGQPGCEVNTFFNV
                     PAN"
     gene            1886..2608
                     /locus_tag="PKK76_05755"
     CDS             1886..2608
                     /locus_tag="PKK76_05755"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNL68007.1"
                     /translation="MDSEFRLKIPFFERRRRIRLPWTILIFGVIFLSLPVMNYIALGQ
                     RLSIPWKYPRTLLGALNPFEIVLLLLPIVIGVGLLMVKRWAWWLFLAYATTLLAYNAA
                     ILALSRQLYNVNALVQTAIFVSAVFYFLRRDISAPYLRMYPRGWRLQKRKPIVVEVIV
                     DGIRRTTLDVSDTGIYVDWEDCYRSPGEEVHLTFRIEGHEYKCRAGVVRVDEDGAGIA
                     FRGVTASFRKELARDLESLAHG"
     gene            complement(2617..4938)
                     /locus_tag="PKK76_05760"
     CDS             complement(2617..4938)
                     /locus_tag="PKK76_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HNL68008.1"
                     /translation="MARNLIIIGGGTLQMPLIETAKAMGLFTIVFDMTLEAPGMKAAH
                     LPVTMSTRDIDGCVREAKRLRETMQIHGVVTAGTDASRAVAAIAGALELPGIRYADAE
                     AASNKVLMRKRLKQQGVPGPDFRSVWSIKEAREAMDELTFPLVLKPADNMGARGVIKI
                     KTRDEIYSAFRHAKKYSPTGEMILEEFMEGPELSIDALAWKGHIRLTGVADRIIAREP
                     YFVELGHNMPSSLPASVLEEAEEVMKAGMRALGIHTGAGKGDIKVARLPAGQKNSRHA
                     TPYDDYAVKVGEIAARLSGGFMSSHTFPLHSGINLHKAAIQIALGDEPSSLEPTRNLV
                     AIERGILSSPGKILELNGVAEMSQVKGIEHIFFTKKTGEIIQEATSNLDKVGHVIAVA
                     PSLEEAEAAAQECLRRVQITVDDTFSVDWKAVEEKARTRFTDQVCWVCKSCVGNCASA
                     VPGMGGVGNMTTFRDNSNALGEIRVIPRYIRGQIQPDTTFEFLGRKFELPIMSAPMTG
                     TATNMNAAISEYDLARILIHGARESGSIAWVGDGATPDRYKTIFRALSEADSFGIAIC
                     KPRADEAALRERFKAAEELGLIAVGMDIDAISFRTMELKKLGATARNYEALRMLRDSV
                     KLPFILKGIMSPEDAEEALRAGVSAIVVSNHGGRVLDDMPGTARVLPEIVRAVGGRIP
                     VLVDGGVRSGQDTFKMLALGASAVLVGRPLGIAAVGGDIAAVKYVFSRYREELARTMN
                     LCGTEKLTEIDSGFIRTYTTEREEADRSSHTEG"
     gene            complement(5013..5957)
                     /locus_tag="PKK76_05765"
     CDS             complement(5013..5957)
                     /locus_tag="PKK76_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017853989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNL68009.1"
                     /translation="MNGSQEITWTLHKENPLIAPPWPSPIIADPSLLLPETSPDGKWH
                     LIAHSILGLHSFTSEDGIHWSRPSFLFANAMRPYLYQEGETYYLFYERYRSFQLPFSW
                     VPFPKWKSRIEVRRSKDLKHWSKPHTVLLPHLSWHESKYGKSISNPCLIKVEKIYRLY
                     YSASLERIEDCGFCEPRYIGYAESEQITGPYTSATEPMITPEVSDPHCNLAAGAMKVV
                     EHNGQYLGFQNGIYVDERSVSGSAILLLKSADGITWSRPTEPILKPTSGWMRSHVYAL
                     DVKRRSKTWYLYFNARDDWHWTRGKEKIGLLLGEEAVP"
     gene            complement(5970..7268)
                     /locus_tag="PKK76_05770"
     CDS             complement(5970..7268)
                     /locus_tag="PKK76_05770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNL68010.1"
                     /translation="MRIHKICLLSCFFLLCSVIQSLHCETRDSFLVVQTGWTTAADTI
                     SQEELRRSFCAGSVRSSASAWPAASALFGCKGAEPAKVEAFYPASREKLILLDLSETT
                     PRMKALKVDGLSPFADGEKYPLRGKSLPRFTHFILTGVSALTRYTGKSADANGVSVLS
                     EQVRPFFQSADFVHVSNEVSFTSPCVFEKGTRFCSKEAHFQVYRDLKVNVVELTGNHN
                     RDYGNEPFFRTYDWFHAHGMNTFGGGKTEASANEPLVLNANGTRIVLIGFNELCPLNE
                     CAQGSTPGANRFEAEKAHRTVTDIRKKYPDAFVIATVQYGEVSAYKPTESQKRISYAL
                     LDSGADLAYGSQAHQVQQMEFRKGKVILHGLGNFLFDQTHTFGLRQGYFMNLYFQGNR
                     LAGMEPVFTWIDEKFRPVPATPAQAREIKSAIYSDDLLYK"
     gene            7257..>8216
                     /locus_tag="PKK76_05775"
     CDS             7257..>8216
                     /locus_tag="PKK76_05775"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL68011.1"
                     /translation="MDAHMRFLNSPSSIQATSFLRSTGGPARSVMRCAILFVFIAAAC
                     SPRTGLPVAAGGVLDLSKMTYSEPIELEGEWGFFRNRFLPPTEDAAADRTMTVPGVWP
                     SHGEPGTGFATYRLKVLLPPGLRRPALFFAHTETAYSIYADGRLLGSAGTPGETEATT
                     TPEWRPRVFEIPSDSSEVVLVIHLANFHHARGGIGYGAYIGSMEHITLMRERELGRDA
                     FLVGILLVLALYHGIIFFLRRKDPAPAYFALLCLNLAARAAFTGSTPILLGLDSIPYN
                     VHARIVYLSFYLAAPAFAAYIAAIYSDTILIWSQRATVAIALLF"
BASE COUNT         1793 a         2093 c         2135 g         2195 t
ORIGIN      
        1 tgatcgatgg atcaaagcaa tcttacggtg ctactgtggc cggctacacg aatcaagggt
       61 atgccctttc cggtttgatt gtagtcatgc taaacaatcg caacaatgcg ggggatgcag
      121 tggcacacgt caccactact tcagcggccg gcacgtttac attcacggct cagcacgtag
      181 ttctgcctgc aggtttgaca gatgattctg cccgggtggc ctatgctgct tctcaattcg
      241 cgggcggcaa tgttacgacg gcgaatattt cgggaactga ctctgtttcg gactccatca
      301 caacagatgg aacgaactac tggttcaagt ctaacagcta ttccatctac atcatagacc
      361 cgcgcggtca tatgagcgga tctctgacga ctgccaccaa cggttctgtc gcaagcaaca
      421 ctcctactgc tgccactacc ctgagcgtgg gcgcaatcgc cgcgcacctg ccccgtggca
      481 aaatttccgg agttgtaacg gatgcattct cgacagaggc tatttccgga gccctcgttc
      541 agctcggacg cgatacaaat ccggatccgt ctctgatttc tttttcgcct gctagaaagg
      601 attcaaatcc aatcgcgaac gtgagcagac tcagcaattc agcggacgtt caggttgctg
      661 acgtaaccac tccttcgaac ggatcttata gttttgacaa tattgatcca ggcgattacg
      721 tgctgcagat ttcaaaaaat ggatatgtta cgggtacaat accagtcaca gtgccgtcta
      781 acggtggttc tgtgtccgtg aatccaccga tcgtcgtgga tggacccaaa ggaaatttga
      841 ccggctatgt gaagcttccg ggcggattta atttcaccga tccgtattca cttgaagtca
      901 ctcacccggc gtctggaatt catccgccag ccggagtgat tccggcaagc ctggcatccg
      961 ggcccagcca gtttgcaaac gcgccacagt attccatctt cgcactacct gcgggagtct
     1021 ggaaattgaa gttcgtttct gccggataca aaacagtaga aggaatcgtc acgattcagg
     1081 gaaatgcaac tacgaacttt gacatcatta ccatggttcc aggatcgcat cctccggctt
     1141 ccattggtgg cagagcactg aacgcactga acaatcagcc gatctcttct ctgactgtgc
     1201 gtcttcgccc aggagttgga gttacaacgg gtgcttacgc aatcgatgcg aacggtgcta
     1261 ctcttccagc cgtggtgaca ggttccaacg gagctttcgt agttgcgaat gttccggcgg
     1321 gcaactatac tctggaagta tcaggttcag gctattccac tacgtatcgt accgtgatct
     1381 ctgctggaac agacacacct gcagcgcagg atattctcgt atctccagtg ttaggtgcca
     1441 atgaagtgag aattgtttta tcatggaacg caacgcccaa agatctagac tcccacctgg
     1501 agtatggatc aggctggccc aatcaagttg tctggaatga tcgtacgaag ttgagcggcg
     1561 atctgcttct ggactgggat gttgtcacag gatatggtcc ggaaacagtg actatgaaag
     1621 gaactgtgtg gacacagccg cgaagagggt acagtgtcta caactggagc cgtggcaaga
     1681 accgtgatac tgtgctcacc atcgggcaat caggcgcgac cgtgaaagtc tttaaatcgc
     1741 agggacttgt gcgctcagtg gcagctggac cttctcaggt cagcgattgg tggcagatgt
     1801 tctgtttcag cagtgctcag ggattgattg atctgggaca gcccggatgc gaagtgaata
     1861 cattctttaa tgtgccggca aactgatgga ttcagaattt agactaaaga tacctttctt
     1921 cgaacgccgt cgcaggattc gtctgccatg gacaatcttg atctttggag tgatctttct
     1981 gtcgctgcca gtgatgaatt acatcgcact gggccagcga ctgagcattc catggaagta
     2041 tcctcgcacg ttgcttggag ctttaaatcc gtttgagatc gtacttctct tgcttccgat
     2101 tgtaattggc gttggactac tgatggtgaa gcgctgggcc tggtggttgt ttcttgccta
     2161 cgcgaccacg ctccttgctt acaatgctgc catcctcgca ctttcgcgtc agctttacaa
     2221 cgtgaatgct ctcgtgcaga ctgcgatctt tgtttcagcg gttttctatt ttctgcggcg
     2281 cgatatttct gcgccttatc ttcgcatgta tccgcgcgga tggcgtctcc agaaaagaaa
     2341 gccaatcgtt gtggaagtca ttgtggatgg aattcgcagg actactctcg atgtcagcga
     2401 tacagggatc tacgtcgact gggaagattg ttatcgatcg ccaggcgaag aagttcacct
     2461 gacgtttcgc attgaaggcc atgagtacaa atgcagggca ggtgtagtcc gagtggacga
     2521 agacggcgca ggcattgcat ttcgcggagt gactgcttcc tttcgcaaag aactcgccag
     2581 ggatctagag tctctcgctc acggctgatc gcctggtcac ccttccgtat gcgaagatcg
     2641 atctgcttct tcgcgttctg tagtgtaggt tcggatgaag ccagagtcga tttcagttag
     2701 cttttctgtt ccacacaggt tcatggttcg ggccagctct tctcgatagc gagagaagac
     2761 atatttcaca gcagcaatgt ctccgcctac agccgcaatt ccaagcgggc gtcccaccag
     2821 gacagcgctg gcacccaggg ccagcatctt aaaggtatcc tgaccgcttc gcactccacc
     2881 atccacgagt acaggaattc taccaccaac tgctcttaca atttcaggca acacacgcgc
     2941 tgtgcctggc atatcatcca gaacgcgacc tccatggttg ctcactacaa ttgcagaaac
     3001 tccagcacgc agtgcctctt ctgcgtcttc cggtgacatg attcccttca atataaaggg
     3061 cagtttgact gagtctctca gcattcgaag tgcttcgtag tttcgcgcag tggcacccag
     3121 cttcttcaat tccattgtgc gaaacgaaat cgcgtcaatg tccattccaa cggctatcag
     3181 ccccagttct tctgctgctt tgaatcgttc gcgaagagca gcttcgtctg cccttggctt
     3241 acaaatcgca attccgaatg aatctgcttc tgatagtgct cgaaagattg ttttgtatcg
     3301 atcaggagtg gcaccatcgc ccacccaggc gatgcttccg ctttcgcgtg cgccgtgaat
     3361 caaaatgcga gccaggtcat attcagagat cgcagcattc atgtttgtag cagtgccagt
     3421 catgggtgcg gacataatgg ggagctcaaa ctttcttcca aggaactcaa acgttgtgtc
     3481 cggctgtatt tgaccgcgaa tgtacctggg aatgacgcga atttctccaa gtgcgttcga
     3541 gttgtctctg aatgtagtca tgttgcctac accgcccatt ccaggaacag cgctcgcgca
     3601 atttcccacg catgatttgc aaacccagca gacctggtct gtgaatctgg ttcgtgcttt
     3661 ttcctctacg gctttccagt ccacgctgaa tgtgtcgtcg actgtgatct gaactctgcg
     3721 caaacattcc tgggctgcgg cctctgcttc ttccagagac ggagcgacgg caatcacatg
     3781 gccgactttg tccagattgc tggttgcctc ttggatgatt tcgcctgtct tctttgtgaa
     3841 gaagatatgt tcgattcctt tcacctggct catttccgct acgccgttga gctcaagaat
     3901 ctttcctgga gaagatagaa tgcctctttc aatcgcaacg aggttgcgag ttggttccag
     3961 agaagaaggt tcatctccaa gcgcaatctg gatggccgct ttgtgtagat ttatgcctga
     4021 atgaagcggg aaagtgtgcg aagacatgaa accgcctgac aaacgtgcgg cgatctcgcc
     4081 gacttttacc gcataatcat cgtaaggtgt cgcgtgacgt gaattctttt gtccggctgg
     4141 aagtctcgcc accttgatgt cgccttttcc tgcgccagta tgaatgccga gcgcccgcat
     4201 cccggctttc attacttcct ccgcctcttc aagtacgctt gcgggcaaag acgaaggcat
     4261 gttgtgtccg agttccacga agtatggctc acgagcgatg atccggtcag ctactccggt
     4321 gaggcgaatg tgacctttcc aggcgagtgc gtctatagaa agctctggcc cttccatgaa
     4381 ttcttcgagg atcatttctc cggtaggact gtacttcttt gcatgacgaa atgctgagta
     4441 gatttcatcg cgagttttga ttttgataac gccgcgcgcg cccatgttgt ctgcaggctt
     4501 gagtacgagt gggaaggtga gctcgtccat ggcttcgcgc gcttctttga tggaccatac
     4561 ggatctaaaa tcaggaccag gaactccctg ttgcttcaat ctttttcgca tgagtacttt
     4621 gtttgatgct gcttctgcat ctgcgtagcg aatgcctgga agttccaggg cacctgcgat
     4681 cgcggcaaca gcccggcttg cgtctgtccc ggctgtcacc acgccgtgaa tttgcatggt
     4741 ttcgcggagg cgtttggctt cgcgcacgca tccatctatg tcgcgtgtgc tcatagtcac
     4801 cgggagatgc gccgctttca ttccaggggc ttccagggtc atatcaaaga caattgtaaa
     4861 aagccccata gcctttgcgg tttcgatgag aggcatttga agcgtgccac cgccaatgat
     4921 gatgaggttt cttgccatga aatggtcctc cgggtctctt ataaaggtcg gcaggaacgg
     4981 catttatatg aggagaaatg gcttctggcg ggttacggga ccgcctcttc gcccagcagg
     5041 aggcctattt tttctttgcc cctggtccag tgccagtcgt ctcgtgcgtt gaagtagagg
     5101 taccatgtct tgctgcgacg ctttacgtcc agggcataca cgtggcttcg catccagcca
     5161 ctggttggtt tgaggatcgg ctcggtgggc ctggaccatg tgattccgtc tgccgatttc
     5221 aggagtagaa tggcggatcc agagacactg cgctcatcca cataaatgcc gttttgaaaa
     5281 cccagatact gcccgttgtg ctccactact ttcatggctc cagccgcgag attgcaatgt
     5341 ggatcggata cttccggagt gatcattggc tctgtggcgg aggtgtatgg tcctgtgatc
     5401 tgttcgcttt ctgcatatcc aatgtatcgc ggttcgcaga acccacagtc ttcgattctt
     5461 tccagggatg cactgtagta caatctataa attttttcaa cttttataag acatgggtta
     5521 gagatagatt ttccatattt cgactcatgc catgaaagat ggggtagcaa gaccgtgtgc
     5581 ggtttgctcc agtgcttcag gtctttcgac cttctcactt caatgcgtga tttccatttt
     5641 ggaaaaggaa cccaggagaa aggaagctgg aaagatcgat atctttcgta gaatagatag
     5701 tacgtttccc cctcctgata cagatacggc cgcattgcat ttgcgaatag aaaagagggc
     5761 cgactccaat ggattccgtc ttcggatgtg aatgaatgga ggcccagaat ggaatgggcg
     5821 atcagatgcc actttccgtc gggtgatgtc tccggaagaa gaagagaagg atctgcgatg
     5881 ataggcgaag gccagggcgg tgcaatgagc ggattttctt tatggagagt ccaggtgatt
     5941 tcttgagagc cgttcacaaa acaccgagcc tatttgtaca gtaaatcgtc ggagtagatg
     6001 gcggatttga tttcgcgtgc ttgagccgga gtggctggaa caggcctgaa tttttcgtca
     6061 atccaggtaa acactggttc cattcctgcg aggcggttcc cttgaaagta taaattcata
     6121 aagtatccct gtctgaggcc aaacgtgtgt gtctggtcaa acaggaaatt tccgagtccg
     6181 tgaagaatga ctttaccttt tcgaaactcc atttgttgga cctgatgtgc ctgggaaccg
     6241 tatgccagat ctgcgccaga gtccagcagc gcgtagctga tgcgtttttg tgattccgtt
     6301 ggtttgtatg cgctgacttc tccgtactgt acggttgcaa tgacaaacgc atccggatac
     6361 ttctttcgaa tatcagttac tgtgcgatgg gctttttccg cctcgaatcg attggcgccc
     6421 ggagtggatc cctgggcgca ttcattcagc ggacagagtt cgttaaaacc aatcaataca
     6481 attctcgttc cgttcgcgtt caacaccagt ggttcgtttg cggatgcttc tgtttttccg
     6541 ccgccgaaag tgttcatgcc gtgagcgtga aaccagtcgt atgtgcgaaa gaatggttcg
     6601 tttccgtagt cgcggttgtg gttacccgta agctcaacca cgtttacttt caggtctctg
     6661 tatacctgga agtgagcttc ctttgagcaa aacctggttc ctttttcaaa cacgcaggga
     6721 gatgtaaaac ttacttcgtt gctcacgtga acaaaatctg cgctttgaaa gaatggtctg
     6781 acctgttcgg agagaaccga cactccgttt gcatccgcgc tcttaccagt gtatcgagtg
     6841 agcgcgctta cgcctgttag aataaaatgg gtgaaccgcg gtagggactt accgcgaaga
     6901 ggatattttt caccgtcagc gaacggcgaa agaccgtcca ctttcagtgc tttcatacgg
     6961 ggagttgttt ctgaaagatc gaggaggatg agtttttcgc gcgaagcagg atagaacgcc
     7021 tctactttcg cgggttctgc tcctttgcag ccgaacagtg cgctcgctgc cggccatgcg
     7081 gatgcagaag agcggacgga tccagcgcaa aaactgcgtc gaagttcttc ctgcgaaata
     7141 gtgtccgccg cagtggtcca ccctgtctgt acgaccagga atgaatctct ggtttcgcag
     7201 tggagactct ggatgacgga acacagtagg aagaagcaag agaggaggca gattttatgg
     7261 atgcgcacat gcgattcttg aattccccct catccattca agcaacatcc ttcttgcgtt
     7321 ccaccggagg acctgcacga tcggttatgc ggtgtgcgat tttgttcgtt ttcattgctg
     7381 ccgcctgttc tcctcgaacg ggtttgcctg ttgctgcggg cggcgtcctg gacctgagca
     7441 aaatgactta ctcggagccc atagagctag agggcgaatg gggatttttt cgaaatcgtt
     7501 ttcttcctcc aacggaggat gctgctgcag atcggaccat gacagttccc ggagtgtggc
     7561 cctctcacgg agaaccgggg acaggctttg ccacgtatcg tttgaaagtc cttctcccgc
     7621 ccgggctgcg caggcccgct ctcttttttg ctcacactga aacggcttac tcgatttacg
     7681 cggatggtag attgctcgga tcggcgggaa ctcctggaga aacagaagca actacaacac
     7741 cggagtggcg gccgcgcgtt ttcgagatcc cttccgattc ttcggaggtg gttcttgtga
     7801 tccatcttgc gaacttccat cacgcgcgcg gaggaattgg ctacggggcc tacataggct
     7861 ccatggaaca tatcaccctc atgcgagagc gcgagctggg tcgtgatgca tttcttgtgg
     7921 gtattttgct tgttcttgcc ttgtatcacg gaatcatttt ttttcttcgc cgaaaggatc
     7981 ctgcgcccgc ttactttgcc cttctgtgtt tgaatctggc cgcgcgtgca gcttttaccg
     8041 ggtcaactcc cattctgctt ggactggatt caattccgta taacgttcat gcaaggatcg
     8101 tttatctttc tttctacctg gcagctcccg cgtttgccgc atacattgcc gccatctatt
     8161 cagatacaat tctgatctgg agtcagagag cgaccgtcgc cattgcactc ttattt
//
LOCUS       DAORFF010000100         8205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4258_length_8205_cov_3.786258, whole genome shotgun sequence.
ACCESSION   DAORFF010000100 DAORFF010000000
VERSION     DAORFF010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4258_length_8205_cov_3.786258"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5770)
                     /locus_tag="PKK76_05780"
     CDS             complement(<1..5770)
                     /locus_tag="PKK76_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL68012.1"
                     /translation="MRRVLFVCILLAACKTIPPVDPPEFDYPGLARLAETAPGRTIPL
                     TIQRGANLHPVSSQTGFLFYSSNRDGSGDIWMRDLHNTVAFPVIKHPAEQGKPAVPLN
                     GQKLAFVSFDTDAGGDIRIVEIKPDSIIKDSLAGSTPPNLWDSSDSLSEQVVQASKQL
                     PPACRGAFAETDPVFSGDGKYLYYVSDRCTPGTFNIWRIELKGIDLKGTPEIISQKGG
                     VQPALSYDGKRITYVSYRSGHAEIYVQDLQTRQEILVRTGGGILQYPALSPDGAIVFY
                     IAVREDTNQNGSLDAMDKAGIYSAPVAGGREARLVDPSTSLHGLYFSLFLGGTLLYSA
                     ESYQNISVYFVRPEGVIPRQADIEEQYLFASRFLREPGKRTIALDALERFFGGTKEFI
                     LYEGKYLQSRVDTLIGDERKAAEEELKEARARNPFAEYQLTLAALPRQKSRVEFIQEF
                     LKKTESSDLQNEQRTLIVPAVLDDLSKAQESEGDKPGALESLVRIDKEFPTYYRRARI
                     RLREAVLELKVTGKIPAVAMEELQAKPSIQRREEILTEIVNYFGRIRKPDDLDAAVSK
                     ELERADLIPLIRSTLEYLRARSLYDRGRDEEALTLARNLQTGLPQESGMYVRTWQLLA
                     FLFERRRDYAGAYEARLRYGGAYRPDTGVKIGLDEYREILEQSDRTIKRTLASARSIA
                     AVVDEQDAVISRTLLSRTLEGTPVQTLLNQVRQNPIELSASDREALHDLCFGNTGAII
                     GNLGKPEYVKTFVQFCKDAEPYLKGKNEGGMNLEFARTGSDLLYLVSYSNVNILNILF
                     YNMKRVNLFEKFHEENSIKYHRLKVDIAMERNRNRIEWDRRQVAILDPRNVIDLFSEG
                     DPFDATSFNEVVHGYKFAATEARSVGELSFVYGYAYALIQKSVERDRFYDRSAKEGIT
                     IPEDVRRAKKQDTLRDLKTAEYLLQYILFVKPDNADAYLQLGWLYQYIDDRRSRNIPT
                     AETFVVRVVNYLTRTSAPRATDGQYYIDIYRAYFPDRLYENNVELYRQAIQLIGFLNS
                     TDPALPDLHLNLANNYFQLLNFKKAIDHFNQNEDLRRAVGGASDPVRTGLFYFNRGRA
                     YYYEGQTTSASRDLKKALEMYTSSDLSPAEELVEGIRFRITDVGEKSENMARLKVRLE
                     DGQKRLDNARLRVAMIGALYALSLYESGRYAESSRAYETADAQIRRMQEPANELRAGI
                     LNFLALARQNQSRIESSDIQAIASRDLSKEIGLSRQDARYQPQSIGGRLLGCLLTYGE
                     DFSVIGEGRNPYGLAPLRQYELSLGILLENRILAGDRSGAFEILSQRREIFDGKDGDV
                     NLGRIGLITADNQRAYEEFLSGHYESAQKLFATAGENARAHGFLDQARRNYINRYQAL
                     FALFESGDDALPRMKGALADLDEFRSVYREKAKEEYISQKQTEDPAFEYEPAIDDPIL
                     ESQLTRRLQDFVVVEANLHYYSGYSLDRRATSPADLTQARAHYTKALELYDRLIAGQQ
                     DEATSPVHFRVRLNRGVILSRLGRIEQAMNVLSRMIEELEEFALSGEEWFARRALANL
                     AAARFFERGRPSDAALAREQYIRAAEILTEDPTVRKGLIGMQEFYEDAADFFASSGDP
                     QKANQLLEQSWVYALQEDYLRYPISFESTALTETYEKYLSASRTARALAVREQSLRFG
                     RKDSKVAREEKMRALASLETARESMRSAVPLHEAFLSSPVSSPDLRKGQWMIRLFYRG
                     HTLSVFAFLPGFAYYREDGNPEAALKTILNRLPRRVPSALLVIADQETWSLNLEAAIQ
                     SARPDLPVPLFVTRMSDPGVLYSEIRPELNQFNTLDANSDFEASDVFIVEREFADDFF
                     AIENGRFQARQFLQKRHNASMAVTDEPSRNFNKIRTAYELFRAGGVGSLVIRQSKGPL
                     PAQNQKLTGQGFRVMGSEGFRTD"
     gene            complement(5767..6393)
                     /locus_tag="PKK76_05785"
     CDS             complement(5767..6393)
                     /locus_tag="PKK76_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68013.1"
                     /translation="MKGLCIFLALWAFASCAFLQKNLKDPAVNLYRRGSCVSIDIPPI
                     TLTPARTAAERQLIGEDREIEKDGWLLASARSTTRAEESVVLQESRRIYREQAVLEFY
                     EESLREYARAGVLGEDTDRAEVVIVPERIAPRTARFRSPEEMDLARRTADEINKSRSY
                     IIQYYLKQEKEKPGSDVSLVEKSLRQSFLKNLRPGDWVMSNGNWIRQK"
     gene            complement(6390..>8205)
                     /locus_tag="PKK76_05790"
     CDS             complement(6390..>8205)
                     /locus_tag="PKK76_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68014.1"
                     /translation="FHSLLVPDLFVSYNNATIRGNAKIMPGEGVLVDLIVSRLRLAPF
                     AGPSMDGVASGQIRLQSPESFRDLDVKSELHVSETRLRMQDSRSAPLELGLVANGKIG
                     TGDTTTVSMDKLLLTVQNQSGVNLLTLNSATDMSFGELQNIQFHIKDLTVRYADLHSS
                     LPGNLRYTLAPYRSYLDDGVKVSGSFGMRGTKDVTNITTDMMLAMPGIHLMDLAVHTS
                     VSLSEKKTLIEDLKMTGLRGALLGTVKGSINKVDGKNVPDLTVNLGLSSKTLFAIHKN
                     LALQGGLLMDFKIQADSVRGNVNANDLSLEIYTDCDAQSACKKMRIEKMNLALPIFHD
                     LKIARPALLADSPAGLTFDAAQFRGDANLTIQFIASTHNPRGAIVKDGYFYSGSLLPG
                     RGPGVSASLEYRKNVLYIKWLRYQIFRPKAREGNDVWVADGRIEGRDVFFNLADLSPR
                     SMEFGGVMQIQNFDLEPYLPASRSNYDGVISAEMGFRGKDLGNALYNSDLRLSVYRLS
                     REFSGFATRVVMPGQVAGFIVKSALEIPSITLELQNGLVYTTIGIARKDVRSFTTALS
                     FIMKPTGEEIRQERIPLAQFLDRARSEVEAGVKVEERQ"
BASE COUNT         1991 a         2163 c         2023 g         2028 t
ORIGIN      
        1 catccgtgcg aaagccctca gatcccatga cgcgaaatcc ctggccggtc agcttctgat
       61 tttgagcagg cagcggccct ttgctctggc gaatgaccag cgagccaact cctccagcgc
      121 gaaacaattc gtaagcagta cgtatcttat tgaaattgcg agagggttca tccgtcaccg
      181 ccatgctcgc attgtgtcgt ttctgcagaa attgtcgcgc ctggaaccga ccattttcaa
      241 ttgcgaagaa atcgtccgca aactcacgct ctacaatgaa cacatccgag gcctcaaaat
      301 ccgaattcgc gtccagcgta ttgaattgat tgagctcagg acgaatctct gaatacagga
      361 cacccgggtc gctcatcctg gttacaaaca gagggacagg cagatccggt cgggcactct
      421 gaatcgctgc ttccaggttt agagaccagg tttcctgatc tgcaatcacg agaagggcag
      481 atggtactct tcgaggcaac ctgttcagaa tcgttttcag ggctgcttcc gggttcccgt
      541 cttcgcggta gtaggcgaat cctggcaaaa acgcgaacac ggatagagtg tgtcctctgt
      601 agaaaagacg aatcatccac tgccctttgc ggagatcagg agaagagaca ggtgaactca
      661 ggaacgcttc atgcaacgga acagcagacc gcatagattc gcgggcagtt tcaagagaag
      721 cgagcgcgcg cattttctcc tctcgcgcga ctttcgaatc ctttctccca aatcgtagcg
      781 attgttcgcg gactgcgagt gctctggcag tgcgagaagc actcaaatac ttttcgtacg
      841 tttctgtaag tgccgtggat tcaaaagaaa tgggatagcg aagatagtcc tcttgaagag
      901 cgtagaccca ggattgttcc agaagctggt ttgctttttg gggatccccc gacgaagcaa
      961 agaaatcggc tgcatcttcg tagaactctt gcatcccgat cagccctttt cgcacagtcg
     1021 ggtcttctgt tagaatttcc gctgcgcgaa tgtattgctc gcgtgcaaga gctgcatctg
     1081 atggccttcc gcgctcaaag aatcgagcag cggccaggtt tgccagagca cgacgcgcaa
     1141 accattcctc tcccgaaaga gcaaactcct ctagctcctc tatcatgcgc gaaagtacat
     1201 tcatggcctg ctcgatgcgc ccgagccttg aaagaatgac tccgcgattc aggcgaacgc
     1261 gaaagtgaac gggagaggta gcttcgtctt gttgtcctgc gatcaaacga tcatagagtt
     1321 caagtgcttt cgtgtagtga gcgcgcgcct gcgtcaaatc tgcaggactt gtcgcgcggc
     1381 gatcgagaga gtagccggag tagtaatgaa ggttggcctc aacgaccaca aagtcctgga
     1441 ggcgacgggt aagttgcgat tccaggatcg gatcatcaat ggctggctca tactcgaagg
     1501 ctgggtcctc cgtctgcttc tgggagatgt attcttcctt cgctttctcg cgatatacag
     1561 atcgaaattc gtccagatct gcaagcgcac ccttcattcg ggggagagcg tcatcaccgc
     1621 tttcaaacaa agcgaaaaga gcctggtagc gattgatgta gttcctgcgg gcctgatcca
     1681 ggaaaccgtg agcgcgcgca ttttcgcccg cggtcgcgaa aagcttttgg gctgattcat
     1741 aatgaccgga taagaattct tcataggctc gttggttgtc agcagtgatc aaaccaatcc
     1801 gcccaaggtt cacgtcaccg tctttgccat caaaaatctc acggcgttgc gaaaggatct
     1861 cgaaggcacc gcttctgtct ccagctagaa ttctgttctc taaaagaatc ccgagtgaaa
     1921 gttcatactg acgaagaggt gccagaccat acgggtttct accttccccg atcacagaga
     1981 aatcctctcc gtatgtcaga agacaaccga gcaacctccc tccaatcgac tgcggctgat
     2041 agcgcgcgtc ctgcctgctt aaaccaatct cttttgaaag gtcacgtgaa gctatggctt
     2101 gaatatcaga gctttcaatc cgactttgat tttgacgcgc cagtgccaga aaattgagaa
     2161 tgccagcccg cagttcattt gccggttcct gcattcggcg aatctgcgcg tccgccgttt
     2221 cataagctcg agacgattcc gcataacgtc cagattcata caaagacaga gcatatagag
     2281 caccgatcat ggccacgcga agtcttgcgt tatccagtct cttctgtccg tcttccagcc
     2341 tgaccttgag acgtgccatg ttttcgcttt tttcacctac gtctgtgatc ctgaatcgaa
     2401 ttccctccac cagttcttct gccggtgaca gatccgagga agtgtacatc tccagtgctt
     2461 tcttcagatc gcgcgaggcg cttgttgtct gaccctcgta gtaataggcg cgaccgcgat
     2521 tgaaatagaa aagacctgtt cgcacgggat cagaggcccc gcccacggca cgcctgagat
     2581 cctcattctg attaaagtga tcaatcgctt ttttgaaatt gagaagctgg aaataattat
     2641 tcgccaggtt caagtgcagg tcagggagcg cggggtccgt tgaattcaag aaaccaatca
     2701 gctgaatggc ctgcctgtaa agctctacat tgttctcata aagacgatca gggaaatagg
     2761 cgcggtaaat atctatgtag tactgaccat ctgtggcacg aggagcgctg gttctggtca
     2821 gatagttgac cacccgaacc acgaacgttt ccgccgtagg aatgtttcta ctccttctat
     2881 cgtcaatgta ctgatacagc cagccaagtt gcaaataggc gtccgcattg tccggcttca
     2941 caaacaagat gtactgcagc aaatattctg cagttttcaa gtcgcgtaga gtgtcctgtt
     3001 tctttgctcg acgcacatcc tccggaatgg tgatgccttc cttcgcgctg cgatcgtaga
     3061 agcgatctcg ctctactgat ttctgaatca aggcatacgc gtatccatat acaaaactca
     3121 attcaccaac actgcgggct tccgtagccg cgaacttgta tccatgcact acttcattga
     3181 aggaagttgc gtcaaacgga tctccttcgc taaaaaggtc gatcacattg cggggatcca
     3241 gaatcgccac ctgacgcctg tcccactcga ttcggtttcg attgcgctcc atcgcaatgt
     3301 caaccttgag tctgtgatat ttgatcgagt tttcttcgtg gaacttttcg aagagattca
     3361 ctctcttcat attataaaag agaatgttga gaatatttac attcgagtaa gataccagat
     3421 aaagaagatc agatcctgtc cttgcgaatt caagattcat acctccttca ttttttccct
     3481 tgagataagg ctctgcatct ttgcagaatt gaacaaacgt ctttacatat tctggtttgc
     3541 ccaggtttcc aatgatggca cccgtattgc caaaacacaa atcgtgcaga gcttccctgt
     3601 cagaagcgga tagctcaatc ggattctgcc tcacctgatt gaggagggtc tgcactggag
     3661 ttccttcgag agtacgagaa agaagtgttc gcgaaatgac agcatcctgt tcgtccacaa
     3721 ccgccgcgat agatcgcgcg gatgcgaggg tgcgcttgat ggttctgtct gactgttcga
     3781 ggatctcgcg gtattcgtcg agaccaattt tgacgcctgt gtccggacga taggctcctc
     3841 catatcgcag acgcgcttcg tatgcgcctg catagtcacg tcgtctctcg aacaaaaacg
     3901 cgagcaattg ccaggttctc acgtacattc cagattcctg gggaaggcca gtctgcagat
     3961 ttcgcgcaag agtgagtgct tcttcgtctc tccctctatc atagagactt cgcgcccgaa
     4021 gatattcgag ggtggagcga atgagaggaa tgaggtcggc tcgttccagt tcttttgata
     4081 cagcggcgtc cagatcatct ggctttctga ttcttccaaa ataattgacg atctctgtca
     4141 gaatctcctc acgtcgttga atggatggct ttgcctgaag ttcttccatg gcaacggctg
     4201 gaatctttcc cgtgaccttc aactcgagga ccgcttcgcg caggcgaatg cgcgctcgac
     4261 gatagtaagt aggaaactct ttgtcgatcc gcaccagaga ctcaagtgcg cctggcttat
     4321 ccccttcaga ctcctgcgcc ttacttagat catcaagaac ggcaggaacg atcaacgtgc
     4381 gttgttcgtt ctgcaaatcg gaagattcag tcttcttcaa gaactcctgg atgaactcca
     4441 ctctgctttt ttgtctgggt aaagcagcca gagtcagctg gtattccgca aaaggatttc
     4501 gcgctcttgc ttccttcaat tcttcttccg cggcctttcg ctcatctccg atcagcgtgt
     4561 ccacgcgcga ttgaagatac ttaccttcat atagaatgaa ctcttttgtg ccgccgaaga
     4621 atctctccag cgcgtccaga gcaatcgttc gcttccctgg ttcgcgcagg aatctggaag
     4681 caaacagata ctgctcttcg atgtcagcct gacgcgggat cacaccttcc ggccgaacga
     4741 aatacacgct tatgttctgg tatgactccg cagaataaag aagggtgcct cccagaaaca
     4801 aagaaaagta aaggccgtgc aaagatgtag atggatccac aaggcgggct tcgcgacccc
     4861 cggcaacagg cgcagaatag ataccggcct tgtccatggc atcgagtgat ccattctgat
     4921 ttgtgtcttc gcgaactgcg atgtagaaca cgatggctcc gtcaggcgaa agagcgggat
     4981 actgaagaat gcctccccct gttctaacca ggatttcctg tcttgtctgc aaatcctgta
     5041 cataaatttc tgcgtgtcca ctgcgataag agacgtacgt tatgcgtttg ccatcataag
     5101 aaagtgccgg ctgcactccg cctttctgcg agatgatttc cggggtcccc ttcaaatcta
     5161 tgcccttcaa ttctattcgc catatattaa aggtccctgg agtgcagcga tcgctcacat
     5221 agtagagata cttcccatct ccggaaaaca ctggatccgt ttctgcgaac gcaccacggc
     5281 aggcaggcgg aagctgtttg gaagcctgca ctacttgttc ggagagactg tcagacgaat
     5341 cccagaggtt aggcggcgtt gatccagcca gactatcttt gatgatcgag tctggtttga
     5401 tttcaacaat gcgaatgtct cctcctgcgt cggtgtcaaa agaaacgaat gctagtttct
     5461 gtccattgag aggaactgca ggcttgccct gctccgccgg gtgcttgatc actggaaatg
     5521 caactgtgtt gtgcaaatcg cgcatccata tgtcgccgga tccatccctg ttggatgaat
     5581 agaacaaaaa gccagtctga gaactgactg gatgaagatt ggcccctctc tgaatggtca
     5641 aaggaatggt gcggccgggt gctgtttccg cgaggcgagc aagaccggga taatcaaact
     5701 ccggcggatc caccggaggg atggtcttgc atgcagcaag aaggatgcat acaaagagta
     5761 cgcgtctcat ttttgcctga tccagttacc gttgctcatc acccagtctc ccgggcgaag
     5821 gtttttgaga aaggattgtc gcagggactt ttccaccagc gaaacgtccg agccgggctt
     5881 ttctttttcc tgttttaagt agtactgaat aatataggat ctggatttat tgatctcatc
     5941 tgccgtgcga cgcgccaggt ccatttcttc cggacttcga aagcgagccg tgcgcggagc
     6001 aattctctct ggaacgatca ccacttctgc gcgatccgta tcttctccaa gaactccggc
     6061 gcgtgcatat tcgcggagcg attcttcata gaattcaagt acagcctgct cgcgatagat
     6121 gcgtctggat tcttgcagga ccacggattc ttcagcgcga gtggtgcttc gcgcggaggc
     6181 cagaagccat ccgtccttct caatttcgcg gtcttctcca atgagctggc gctccgccgc
     6241 ggttctagcc ggagtcagag taataggtgg aatatcaatg gagacacacg atccccggcg
     6301 atacaggttc actgccgggt ccttcaagtt cttctgcaag aacgcgcagg acgcaaacgc
     6361 ccagagggcc agaaaaatgc aaaggccttt cattgccgtt cctccacttt cacgccggcc
     6421 tccacttcgc ttctggctcg atccagaaat tgcgcaagcg ggattctttc ctgacggatt
     6481 tcctcacccg ttggcttcat gatgaagctg agtgctgtag taaaactgcg gacatctttt
     6541 cgcgcaatcc cgatagttgt ataaactaga ccattctgaa gttccagtgt aatggatgga
     6601 atttcaagcg cactctttac tataaaaccc gcaacctgtc ccggcatcac tacgcgcgtc
     6661 gcaaaaccac tgaactccct gctgagacga tacacggaca ggcgaagatc cgaattgtaa
     6721 agcgcgttgc ccaggtcctt gccgcgaaaa cccatttctg cgctgatgac tccgtcgtaa
     6781 ttggagcgcg aagctggaag atatggttca agatcgaagt tctggatttg catcacacca
     6841 ccgaactcca tggacctggg tgacagatca gccagattga agaaaacatc tctgccttca
     6901 attcgcccgt cggcaaccca cacatcattt ccttcgcgag ctttcgggcg aaagatctga
     6961 tacctcaacc acttgatata gagcacattc tttctatatt cgagagaagc agaaacgccc
     7021 ggccctctgc ctggcagaag ggaaccagaa taaaaatagc cgtctttgac gatcgccccg
     7081 cgtggattgt gggtggaggc aatgaactga atggtcaggt ttgcatcgcc acgaaattgc
     7141 gcggcatcga acgtgagccc tgccggagaa tctgcaagaa gagctggccg ggcaattttc
     7201 aagtcgtgaa agatggggag cgcgagattc atcttttcaa tacgcatttt cttgcaggcg
     7261 gattgcgcgt cacagtccgt gtagatttcc agagacagat cattggcatt cacattgccg
     7321 cgcacggaat ctgcctggat cttgaaatcc atcaagagcc caccctgcaa tgcaaggttc
     7381 ttatgaatgg caaataacgt cttactggat aggccaaggt tgaccgtcaa atcaggcaca
     7441 tttttcccgt ctactttgtt gatggatcct tttacagtcc caaggagcgc accgcgaagg
     7501 ccggtcatct tgagatcctc gatcaatgtt ttcttttccg acagtgaaac agaagtgtgc
     7561 acggcaagat ccatcagatg aatccccggc atggccagca tcatatcggt ggtaatattt
     7621 gtaacatcct ttgtgccgcg cattccaaac gagccggaca ccttgactcc gtcatccaga
     7681 taggagcgat agggtgcaag agtgtagcga agatttccgg gaagagatga atgcaaatcc
     7741 gcataacgga ctgttaaatc cttgatgtga aattgaatat tctgaagctc cccgaaactc
     7801 atatcggttg cggaattgag agtcaaaagg ttgacaccag attggttctg cacggtaaga
     7861 agcagcttgt ccatggagac tgtggtcgtg tctccggttc caatctttcc atttgctaca
     7921 agccccagtt ccagaggagc agatctgctg tcctgcatgc gaagccgcgt ttccgataca
     7981 tgcaactcac tcttcacgtc cagatcgcga aaactctccg gcgactgaag ccgaatctgc
     8041 ccggatgcga ctccatccat agagggcccc gcaaacggag caagtcgtag gcgcgaaaca
     8101 atcagatcga ccagtacacc ctctccgggc atgatctttg cgtttccgcg aatggtcgca
     8161 ttgttataag acacaaacaa atccggcaca agaaggctat ggaac
//
LOCUS       DAORFF010000101         8182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4281_length_8182_cov_3.854067, whole genome shotgun sequence.
ACCESSION   DAORFF010000101 DAORFF010000000
VERSION     DAORFF010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4281_length_8182_cov_3.854067"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3346)
                     /locus_tag="PKK76_05795"
     CDS             complement(<1..3346)
                     /locus_tag="PKK76_05795"
                     /inference="COORDINATES: protein motif:HMM:NF037694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNL68015.1"
                     /translation="MKITRQSIYQAFEQIAVKLDPWILRFLDKSQPFLEKASRGLMRA
                     WLLLTASLSFIWKVFTYPVRAAARASGAIFGTFEYRPSPPVRSILSIKQEGIQFVESR
                     VKVIQKRILNAREARPPVFRTAVMMSAAFALVLGGCSSFQLFKKLTRTNATVSGPEYV
                     KPSDETSPSSVSIHFSNPAVRIDLIGKTADIQMKPSHPGTWTFVDDSTITFVPSQHWP
                     AEKSYSVTLKPEFFSPSIKLDDYQVGFETAPFTVTVASAQFYIDPKDPRIKKAVITLD
                     FSHPVDAKSISDRVSFEYKDGLLSGKPLANSVTLDATEMRAFVHSDIVSIQEKEASLV
                     FNLKSGIHAKDGGGSSDSVHADVPVPGKYTHFFIDSTAVQLVRNESYELDQVLFVETT
                     ASITPEELAKNITAYELPKNRPASPGTSESKNHQWQVKEIDEDTLAHSRKLQFVRADE
                     NTEEGTVHPYKIQAQEGAQVFVRVGKNTRAVGDFLLKEKYESIVRVPDFQREARIMHD
                     GAILSLSGEKRISVLSYDNDALEFEIGRIMPDQINHLISQSYGNFNSPYFSNYNFNED
                     NISERYTETIQLNREKGKRLQYASFDFSKYLSAKGPRLQNGLFYLKVRGVRKKKQDQE
                     TENEGESDEVRIGKLEDKRLVLVSDLGILVKAARSGTRDVFVQSFAQGFPVAGVEVSV
                     IGKNGMAVATQTTDANGHATLANLSEFTREKAPVAFIAKAGSDLSFMPFARDDRKLDF
                     SRFDVGGLQGADSRDKLTAFIFSDRGMYRPGDEIRAGLIIKSGDWKRNLSGLPVEVSI
                     LDPRGIEVFSRLIKLSPSAFEEIKYKTEETSPTGSYQIRAMLVPPKKYQERITLGQAS
                     VRVEEFLPDKLRITTQFSQTPQDTEGWVKPEQLKGQVLLMNLFGTPAQHKKVQATLVL
                     TPRFPAFSGYEGYRFYDPLRTTRSFQQDLGTLETDDQGKVEFPLGLEKYAKGLYWVTF
                     HADGFESEGGRAVSTQSLQMVSPLSAVVGIKSDGDANYIHKGSARAVSIIAIDEKLKP
                     VSLSKLKLRIVEQRPMSSLVKREDGVYEYKSIVKEIPVLDRDLIIPQGGFNQLLPTDQ
                     SGDFVLSIVDESSQELNRFQYSVIGQG"
     gene            complement(3387..4061)
                     /locus_tag="PKK76_05800"
     CDS             complement(3387..4061)
                     /locus_tag="PKK76_05800"
                     /inference="COORDINATES: protein motif:HMM:NF036524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNL68016.1"
                     /translation="MKEKRAKKTQARSQETRETIYEAALVLFQKKGFEDATMREIAAS
                     AGVALGSAYYYFRTKEEVVLEFYKRTLEKDDQIKAYFKEETGLKERITYVIRLRIKQF
                     APFKKFLWVLAKNAVVPGSPLSPFSEESAEIRDKAIAIFEEAVADCKPAPAQLAKELP
                     FLLWLYQLGILLFWVLDQGKTMRKTDILLNTTSEMIVWLVKSSGLPIFGGISKRISTL
                     IQELRD"
     gene            complement(4058..4867)
                     /locus_tag="PKK76_05805"
     CDS             complement(4058..4867)
                     /locus_tag="PKK76_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016756607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNL68017.1"
                     /translation="MKIGTLLLALAFAMPVGAQTLHDMAIQGQEDDLARKVTAKNIDS
                     LDREEKTMLMVAAENGQTGVVRMLVRAGANPAIQDRAGNTALHYAIIRGDEEITRLLA
                     TRGKVLEIQNGNGHRPIDLAQSQSNDRIVEILRASGSMPPHVWSKLTLLLYFLYLIIS
                     LGLTVWVARTLSKNGRIFLVDAFHANEPLADSVNHLLVVGFYLINIGYVTLTLREKIK
                     PIAPEEMVESLSVKVGLVLLILGTMHFFNMFVFSRMRKRTQQKPALQEKRA"
     gene            5732..6382
                     /locus_tag="PKK76_05810"
     CDS             5732..6382
                     /locus_tag="PKK76_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68018.1"
                     /translation="MFRRISILFLLFFAACFDYSESIYFSPDLSGFIEIEYRVPLRAK
                     QDTSLISYFDLEKSDINKRYTRLLSRKTEIENFQKEIEQTDSGRFAKVKYRIAFRAPV
                     ELERILIGSTRVYFFEGKWVVQRRIPSSPPLHADAGRVSRRVHELSRMSLSGHSLNFL
                     IFYPPEYDLFTNHGTIVRPGVESLSIPLSTTQNGGGLVWTMEIKANPLQPVSRGKP"
     gene            complement(6339..7094)
                     /locus_tag="PKK76_05815"
     CDS             complement(6339..7094)
                     /locus_tag="PKK76_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68019.1"
                     /translation="MKLGLLPFLNTQPLEYGFRTDPGRFGTTAVIDVPSRLAGALLEG
                     KIDTALISSVEVLRHPELTFCKTMGVCADSAVRSILYIRSAEDPADSVPDRIWMDEGS
                     RTSQALLLLLLKKVYGASPERSSVPASEVPSHIGPGQAGMLIGDAALSFWDRSDLDRF
                     RIRDLGQWWNELEGLPFVFAVWAYPKSNPVPDETFEWSLSMGLSHMEEIIQKAGRSDA
                     REYLTKNLHYRMTERDLKALTVYRGLLAEAGLL"
     gene            7151..7837
                     /locus_tag="PKK76_05820"
     CDS             7151..7837
                     /locus_tag="PKK76_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68020.1"
                     /translation="MFLIGLLLFGQHGESPGDPGFPWGQGYGDWRVQKSAMILKQVPG
                     EKVIVSKNDLEFEFSFHLKKKIRDVRTSVKEEGGKKKAVFEELILDAAEPVENARLYA
                     VAVKYPKLPLTEDLKESIRKMGAQSIPESDQFSIDLPSTLIQITCSRKKDKRYLERIV
                     FISKVYSAVEKEDADLLKKEADSRIKNAVDERQQEMVKSPPQSWMRSEIIPRNESSLQ
                     VTPELWSETP"
     gene            7834..>8182
                     /locus_tag="PKK76_05825"
     CDS             7834..>8182
                     /locus_tag="PKK76_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68021.1"
                     /translation="MKRTVLLILLFAFACVTKTTTGPDAFSYTDGESFRVQIPLANKD
                     GSKSKKEGTVTHEFREKPAEDSVIVRVIVLETKSPEDLFAPGYESSFLNSCNCSVGKR
                     GVIHVNTWAAREYV"
BASE COUNT         1951 a         2004 c         2064 g         2163 t
ORIGIN      
        1 tgccctgacc aatcactgaa tattgaaagc ggttcaattc ctgcgatgat tcgtccacga
       61 tgctcagaac gaaatcgcca gactgatccg tgggcagcag ttgattgaat cctccttgcg
      121 ggatgatcaa atcacgatcg agcactggaa tctctttgac gatgctcttg tattcgtaga
      181 ctccatcttc gcgcttcacc agactggaca tcgggcgctg ctctacaatg cgcagtttca
      241 atttggagag agagacaggc ttcagctttt cgtcgattgc aatgatggag acggctcgag
      301 cggatccttt gtgaatgtaa tttgcatctc catctgattt gattccgact accgctgaaa
      361 gcggggaaac catttgcagt gattgtgtgc tgactgcgcg tcctccctca gattcaaaac
      421 catccgcgtg aaaagtaacc cagtacaatc ctttcgcgta tttttcgagt ccgagtggga
      481 attccacttt gccctgatcg tcggtttcga gcgtccccag atcttgctgg aagcttcgcg
      541 ttgtgcgaag aggatcatag aacctgtaac cttcgtaacc gctgaacgca ggaaaacgag
      601 gagttaagac gagtgtggcc tgtacttttt tatgctgcgc aggtgttccg aacaagttca
      661 tcagcaacac ctggcctttg agctgttctg gcttcaccca tccttctgtg tcttgaggag
      721 tttgcgagaa ttgagttgtg atgcgaagtt tgtcgggaag aaattcctct acgcgaacac
      781 ttgcctgacc gagtgtgatt cgctcctggt atttcttggg aggcacaagc atggcccgga
      841 tctggtaaga accggtggga gaagtttcct ctgtcttgta tttgatttct tcaaatgcag
      901 acggagaaag tttgatcagc ctggaaaaca cttcgattcc gcgcggatca agaatgctca
      961 cctccacagg cagaccggaa agatttctct tccagtctcc ggacttgatg atcaggcctg
     1021 cgcgaatttc gtctccgggt cgatacatgc cacggtcgga gaagataaat gctgtgagtt
     1081 tgtcgcgtga atccgcaccc tgaagccctc ccacatcaaa gcgagaaaag tccagctttc
     1141 gatcatctct tgcaaatggc atgaaagaca ggtcagagcc ggctttcgcg atgaaagcga
     1201 ctggcgcttt ctctcgcgtg aattcgctga ggtttgcgag agtcgcatgt ccgtttgcgt
     1261 ctgtggtttg agtggccact gccattccat tttttccaat gacactgact tccactcctg
     1321 cgaccggaaa gccctgcgca aacgattgaa caaatacgtc gcgagtgcca gagcgagctg
     1381 ctttgaccag aattcctaaa tccgaaacca gaaccaggcg cttgtcttcg agtttaccta
     1441 tccgcacttc atccgattcg ccttcgtttt ccgtttcttg atcctgtttc ttcttacgca
     1501 ctccgcgcac ttttagatag aagagcccat tctgaagacg cgggcctttt gcagaaagat
     1561 actttgaaaa atcaaacgaa gcatactgca gtcgttttcc tttctcacgg ttgagctgga
     1621 tcgtttccgt ataacgttcg gatatattgt cttcattgaa attgtaattg gaaaagtagg
     1681 gactgttgaa gtttccgtag gattgcgaaa tcaaatgatt gatctgatcc ggcatgatcc
     1741 tgccgatttc aaattcaaga gcatcgttgt cataggatag aacagaaatg cgtttctcac
     1801 ctgaaagact cagaatggct ccgtcgtgca tgatgcgcgc ttctctttga aagtcaggca
     1861 cacgcacgat cgattcgtac ttctctttca acaggaagtc gcctacggcg cgggtgttct
     1921 tgcccaccct gacaaagact tgagctcctt cctgtgcctg gatcttgtaa ggatgaacgg
     1981 taccttcttc tgtgttttcg tctgctcgca cgaattgcag tttacgcgaa tgggccagag
     2041 tatcctcatc tatctccttt acctgccact gatggttctt gctttccgat gtgcccggag
     2101 atgcagggcg atttttggga agctcgtatg cggtgatgtt ttttgcgagt tcttctggag
     2161 tgatggaagc agtggtttcc acaaagagca cctgatcgag ttcgtaggat tcgtttcgca
     2221 caagctgaac ggctgtgctg tctatgaaga agtgagtgta ctttccaggc acaggcacat
     2281 ctgcatgcac actgtcagaa ctgcccccgc cgtctttcgc atgaatgccg gacttcaggt
     2341 tgaataccag acttgcctct ttttcttgaa tggacacaat atctgagtga acgaaagcgc
     2401 gcatttctgt ggcatccaga gtgactgagt ttgccagagg cttgccgctc aataggccgt
     2461 ctttgtattc gaaggaaaca cgatcgctga ttgattttgc atctactgga tgagagaagt
     2521 cgagcgtgat gactgctttc ttgatgcgag gatctttcgg atcgatgtag aattgcgccg
     2581 aagcaacagt cactgtaaag ggcgccgttt caaatccaac ctgatagtca tcaagtttga
     2641 tggaagggga aaagaattca ggcttgagag tgacagagta cgatttttct gcgggccagt
     2701 gttgtgacgg aacaaatgtg atggtggaat cgtctacgaa cgtccaggtt cccgggtgag
     2761 atggcttcat ctggatgtct gcggtttttc caatcagatc aattcttaca gcagggttgc
     2821 tgaagtgaat gcttacggaa gagggagatg tttcatccga aggctttacg tattccggac
     2881 cgcttacggt tgcatttgtg cgagtgagtt tcttgaagag ctgaaaggaa ctgcaccctc
     2941 caagaaccag cgcaaatgca gcggacatca tgactgccgt gcgaaacact ggaggcctgg
     3001 cctctcttgc gtttagaatg cgcttctgga tcactttcac tctggattcc acgaattgga
     3061 ttccttcctg cttgatcgaa aggatgcttc gaaccggagg cgaaggtctg tactcaaacg
     3121 ttccaaagat ggcgcccgaa gcacgagcgg cggcgcgcac tggataagtg aaaaccttcc
     3181 agatgaaaga gagcgatgcc gttaacagga gccaggcgcg catcaggcct ctggatgctt
     3241 tctccaaaaa tggttgtgat ttatcaagga atcgaaggat ccacggatcc agtttcactg
     3301 cgatttgttc gaatgcctgg tagatgctct ggcgggtgat tttcacggcc tggtgtgagg
     3361 aatcgtggag gcctgtgcaa gcaaaattag tcccgcagct cctgaatcaa agtggatatt
     3421 cgttttgaga ttcctccaaa gataggcagg ccagaagatt tcaccagcca tacgatcatc
     3481 tcagaagtgg tgttcagtaa aatgtccgtt ttgcgcattg tttttccctg atccagcacc
     3541 cagaagagaa ggattccaag ctggtagagc cagagaagga aggggagttc ttttgccagt
     3601 tgcgctggcg cgggcttaca atctgccact gcctcttcaa agatcgcaat tgccttgtct
     3661 cgaatttcgg ctgattcttc actgaaagga gagagtggac ttcctggaac gactgcattt
     3721 tttgcaagca cccacaggaa ctttttgaac ggagcgaatt gtttgatgcg aaggcgaatc
     3781 acatatgtga ttctttcttt caggcccgtc tcttctttga agtaagcttt gatttgatcg
     3841 tctttttcca gagtgcgctt gtagaattct agaaccactt cttctttggt gcgaaaatag
     3901 tagtacgcag atccaagcgc cacgcctgca ctcgccgcaa tttcgcgcat ggttgcatct
     3961 tcgaagcctt tcttttgaaa gaggaccagc gcggcctcat agatggtctc tcttgtttcc
     4021 tgagaccgcg cctgggtttt ctttgctctt ttttctttca tgcccgcttt tcttgaagtg
     4081 ctggtttttg ctgggtgcgc ttgcgcattc tgctgaatac aaacatattg aagaaatgca
     4141 tggtacccag aatcaggagc accagaccta ctttcacgct caacgattca accatttctt
     4201 ccggcgcgat tggtttgatt ttttctctga gagtcagagt cacgtatcca atgttgatca
     4261 gatagaatcc aaccaccagg agatgattca cagagtctgc gagcggttca ttcgcgtgaa
     4321 acgcgtccac gagaaagatc cttccgtttt ttgaaagagt gcgcgccacc cagacagtca
     4381 gacccaggct gatgatcaga tacagaaagt aaagcaacaa cgtcagcttg ctccacacat
     4441 gcggtggcat actcccactt gctctaagaa tttcgacgat tcgatcgttg ctctggctct
     4501 gagccagatc gatcggccga tgaccatttc cattttggat ctcaagcact tttccccgag
     4561 tggccaggag ccgggtgatc tcctcatctc cgcgtattat cgcgtagtgg agtgccgtgt
     4621 ttccagcgcg gtcctggatc gcaggatttg caccggcacg cacaagcatt cgcaccactc
     4681 cggtctggcc attttcagcc gcaaccatca gcattgtctt ttcttcccgg tccagggagt
     4741 ctatgttctt cgccgtcacc ttgcgcgcca gatcgtcttc ctggccctga atggccatat
     4801 catgcagcgt ctgggcgccc acgggcatgg caaatgccag ggcaagaagt agggttccga
     4861 ttttcataac tgcctccaat aattgaacat gttcaaaata gacgataggg agttcttgtg
     4921 tcgatataaa aaatttgaac gtgttcaaaa attctgatca gcatgtgcgt ggccgcagac
     4981 cttcagcacc agccaggggc tgtcacagag gtttgggctt cttcatttct gtgcgaagct
     5041 gagaccggtc gtgctgttta ttaccgaaaa catgtagttc attccggatt tcctctcaat
     5101 actacatttt atcaagtttc tggttgacga ttgctttcgc agtctcagcc agagcctatg
     5161 agaaatagca aaaagaaccg ggtattcaaa ttcactgcta ctgttgtagg agccatcatc
     5221 ctttctttca gtgtcctggc ttgcggcggg aagtccaaca aatgctcgtc ttcctcttca
     5281 agtagttcaa gctccagctc tggaagcagt tcgctttgtc ttgatgaccc acttacccaa
     5341 ctactggtgt tgtatgttct cctggatgcg gtgcgtgtca ctgcctgtac gaccaacgtg
     5401 gccgggtgca gcgcgtctca attctcttgc agccagagct cattctgcta cacgagtctt
     5461 actgcgtgcc gaaattcacg cacttgcgga atggaggaag gagggacttt ctcacagtct
     5521 tcgcagagtc tccccccaga gcctggaatt ccaaagtcgt tctgagtggg attgttttcg
     5581 cgccgcaacg acggttgcgc tgcggtctcg cttgattttt ctgctctgtg ttgtccgccc
     5641 cgaaatcccg tcgtgtggaa tatgatgggt gagaggcgga ggacttctga aaaaccattg
     5701 actggctgtc cccagccagc gaggttatac tgtgttccgg cggatctcca tcctgtttct
     5761 gttgttcttt gccgcctgct ttgactattc agagtccatt tacttctccc cggatctatc
     5821 tggcttcata gagattgaat accgtgttcc acttcgcgcc aagcaggaca cttctctgat
     5881 ttcttatttt gacctggaaa aatcggacat caataaacgt tatacgcgat tgctttcgcg
     5941 gaagacagag atcgaaaact ttcaaaaaga aattgagcaa acggattcag gtcgatttgc
     6001 aaaagtcaaa tatagaattg cgtttcgggc acctgtggaa ctggaacgca tcctcatcgg
     6061 cagtacccgc gtttatttct ttgaagggaa gtgggtagtg cagcgcagga ttcccagttc
     6121 ccctccattg catgcggatg caggtcgtgt gtcgcgaaga gttcacgagc tttcgcgcat
     6181 gagtctctcc ggacatagtt tgaattttct gattttctat cccccggaat acgatctgtt
     6241 tacgaatcac gggaccatcg ttcgacccgg agtggaaagc ttgagcattc cactttccac
     6301 cactcagaat gggggagggc tggtgtggac catggaaatc aaagcaaacc cgcttcagcc
     6361 agtaagccgc ggtaaaccgt aagagccttc aggtctcttt ccgtcattct gtagtgtaag
     6421 ttctttgtca gatattcacg ggcatcggat cgccctgcct tctggatgat ttcttccatg
     6481 tgcgaaagcc ccatggagag agaccattcg aatgtttcat ccggaaccgg atttgacttt
     6541 ggatacgccc agactgcgaa tacaaaggga aggccttcga gttcgttcca ccactgcccc
     6601 agatcgcgaa tgcgaaatcg atccaggtcg gatcgatccc agaaggaaag cgccgcatct
     6661 ccaatcaaca tgcctgcctg gcctggtcca atgtgtgatg ggacttcgga tgccgggacg
     6721 gagctgcgct cgggactggc cccatatact tttttcaaaa gcaatagaag caaagcctgc
     6781 gaagtgcgcg atccttcatc catccagatc ctgtcgggta cggaatccgc cgggtcctct
     6841 gcagagcgta tatatagaat agaacgaact gcagagtcag cgcaaacacc catcgtcttg
     6901 caaaaggtaa gttcaggatg ccgcagcact tctacggaac tgatcaaagc agtgtctatc
     6961 tttccttcta gaagcgcgcc tgccaggcga gaggggacgt cgatcactgc cgtggttcca
     7021 aatctgcctg gatcggtcct gaatccgtat tccaggggtt gggtattcag gaaagggagc
     7081 agtcccagct tcatctctgg gctttttctc tggggccggg aaaaaatcca tcgatataaa
     7141 gagagtgggc gtgtttctaa ttggactgtt gctctttggg cagcatggcg aatctcccgg
     7201 cgatccaggc tttccctggg ggcagggtta cggggactgg cgggttcaga agagcgccat
     7261 gatcctcaag caggttccag gcgaaaaggt aatcgtatcg aagaacgacc ttgagtttga
     7321 gttttccttt catcttaaaa agaagatccg cgatgtacgc acctctgtca aagaagaggg
     7381 cgggaagaaa aaggcggtgt tcgaagaact gattttggac gcagccgagc ctgttgaaaa
     7441 cgcaaggctc tatgccgtgg ccgtgaaata tccaaaactc cctcttacag aagatctcaa
     7501 agagtcgatt cgaaagatgg gggcgcagag cattccagag agcgaccagt tcagcatcga
     7561 cttgccttct actctcatac agatcacgtg ctccagaaag aaagataaga gatatctaga
     7621 aaggatcgtc tttatttcta aagtgtactc agccgtggaa aaagaagatg cggatctgct
     7681 taaaaaagag gcggattcca gaatcaaaaa tgcagtcgat gaaagacagc aggaaatggt
     7741 aaaaagccct ccgcagagct ggatgcgttc cgaaatcata ccccgaaatg agtcgtcgct
     7801 acaggtgact ccagaactct ggtcagagac gccatgaagc gcacagtctt actgattctt
     7861 ctttttgcat ttgcctgcgt caccaaaaca actacgggac cggatgcatt cagctacaca
     7921 gacggcgaat cgtttcgtgt gcagatcccg cttgcaaaca aagatggttc caaatcaaaa
     7981 aaagaaggca cagtcactca tgaattccgc gaaaagcccg cggaagactc agtgattgtt
     8041 cgagtcatag tgctcgaaac aaaatcgccg gaagatttat tcgcacccgg ttacgaaagt
     8101 tcatttctca attcgtgcaa ttgctctgtt ggaaagcgcg gagtcattca tgtcaataca
     8161 tgggcggcac gcgaatacgt tc
//
LOCUS       DAORFF010000102         8171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4297_length_8171_cov_3.734722, whole genome shotgun sequence.
ACCESSION   DAORFF010000102 DAORFF010000000
VERSION     DAORFF010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4297_length_8171_cov_3.734722"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PKK76_05830"
     CDS             <1..1041
                     /locus_tag="PKK76_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNL68022.1"
                     /translation="KLPIPGDLEKELNGAGLSAKIFAGVQGNPVESQVTAGVAVFRAH
                     NADSIVALGGGAAMDVAKAITLMAHHPGKLFDYEDVPGTKPVDQTIPFFVTVPTTAGT
                     GTEVGRSTVISDDTTHAKKIMFSPRLLARCVFADPELTLGLPARVTAATGMDALTHLV
                     EAYLANDYHPICDGIALEGVRLVAQSLVQCTEYAKAKDPGSDDHRRVRGMMLNAAMMG
                     GIAFQKGLGAVHSTAHPLSTVFDFHHGLANGIMIPYVMRFNAEAVPERFVTLAQAAGI
                     ANPGPDSFIHWLENLKEQLGIPRTISEAGAKKEGLEDLVRFAVLDVCHAMNPRPVSEG
                     DFRNIYTKAFGS"
     gene            1038..1784
                     /locus_tag="PKK76_05835"
     CDS             1038..1784
                     /locus_tag="PKK76_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNL68023.1"
                     /translation="MSLRIGVSACFFHADPKRPIFKGKTLVYAEASMLQWVMSGGAIP
                     VMIPPPYAGFAIKDFLAEVDGVLLQGGSDVSPTSYGETPLKPEWNGDRVRDDYEKSII
                     ETAIEMDRPVLGICRGLQIMNVALGGSLYQDIREQNSASIVHRDWDIYDDLLHRVRID
                     PSSRLGAMYGEGGMINSIHHQGIKDLSPQLSAEAWCVEDGIIEAVRLKSNKAYCLGIQ
                     WHPEFIRKDQNLLDPAPILKEFLTEVSNRK"
     gene            1781..3247
                     /locus_tag="PKK76_05840"
     CDS             1781..3247
                     /locus_tag="PKK76_05840"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNL68024.1"
                     /translation="MIDLARDFARLTEKLKNSKEISIPLQSLESEEEIFANGLVTKAL
                     AYIGQIRLEGTLHLAVAELLSNAEKALLKRTFAEFFANTADYDNPDFLKSFHRKLRED
                     LRSLRAILKKSKPSIEVNMKIQASGLSLSILTGSMPSLQEDEFIHRAMEAGLRAKSLA
                     DLEALPDTIEAEGRGLAISVFSLKSAGIPGIVYALQGDRACFSISVPAHRPDENFLSR
                     IGSDLIGEIQSLPAFPDTTRKLLEMCNSPDTDLKSIALEIGRDAAIAGELIRVANSGG
                     FAGGRVADIAEAAKILGMSNITGLLMRSAAYAILDERYGVTEELLAHPVRVAVYSRTL
                     ARKYKLSAIADQAYVAGLLHDIGKIVLVHHMKQTGSLSSVEKTSRFRDRRSQVNLEEI
                     ACGADHALIGKLLGEKWNFPDELCMAIGMHHSPLGAPKETRKLAFVVYMANALADYEE
                     GQTEFYSVEPEVLAFFNIATVQAFQMLAGSLNSEYSST"
     gene            complement(3290..4639)
                     /locus_tag="PKK76_05845"
     CDS             complement(3290..4639)
                     /locus_tag="PKK76_05845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNL68025.1"
                     /translation="MEYAARIILSTIPVGLFFALYFRNFVFQRSILWQSKAFFLGMLS
                     SIAAIVIQALLPETASPAVRSFFHAALVEEAIRFTVLYLRIRKSSEGFTVTEGIFDGI
                     LVALGFAFSENLHYSATTSGYVILLRCISSVPLHVFCGGIMAYFLSYRSLCRENRKVW
                     RRLNWYGLRRFELAVFALLIPFLLHGFFDYALFRGDVWNYMLVPILVTGFIVTEYMAA
                     RGMQIFGKNILGALGLDADDMEIIGRQQEYEKWLSDYQDEDREAVEIFRNRWEITPTA
                     LGILFFLIAAAFFIIRFRFPSWLSGSGIDAHAQIALTVVLPAFAAAVLLLRDKINYLY
                     FRETMLRVPSGTLVHIEHERGTSQDTMIFDILPGGAFLSGVDDLSQGDRLHLSISRNN
                     KPSLRVRCAVRWVNNVNRQLPTGALVQFLKPEPGFLLFMMIHALKRIHLRPVTRHAG"
     gene            complement(4673..6052)
                     /locus_tag="PKK76_05850"
     CDS             complement(4673..6052)
                     /locus_tag="PKK76_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LruC domain-containing protein"
                     /protein_id="HNL68026.1"
                     /translation="MKYLASLMIVLAAANCGGGKKKAAMWWMALLGNQTAQTAETTSS
                     AGDSVTTTNNDQTGTTGFSFQTTTTVPVSVTVADSNGPVQGAVVQVQDPSAANTVIFQ
                     AVTNETGQASGTFTVDNTVTEVTLVVDAGGNVVTQPVNTTNLVTCDRDVDYPGDVTIS
                     SGGDQDGDGITDANDAYPSDPTRATKVTIPGSGQSIIAYEDLYPSPGDADFNDYVVKV
                     SNEEDLNAAGKVVRLRGSYTHIARGAGYTHQLKLTLPAAAGALNYTVKQYSAAGALES
                     TTTNHYSNGSGLAILGNSNNTITQSNTSSGQTFTPGKRADIEITFDTPVSQSTLGSAP
                     FDLYIHVNNTGLDVHFPRMYFDANGKDQYMDTNGFPWALQIPEAWKWPLESDNIENGY
                     PGFRPWYLSSGTTNKDWYSSAVSSHVFNASSLFAYLKSPASWMILGLIAAIAVIGLAL
                     AMIQRRRAA"
     gene            complement(6184..7323)
                     /gene="alr"
                     /locus_tag="PKK76_05855"
     CDS             complement(6184..7323)
                     /gene="alr"
                     /locus_tag="PKK76_05855"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNL68027.1"
                     /translation="MHTYVEIRASALKSNLIHFRRATSTGVMAVLKSNAYGHDLELCT
                     RAVRGQADWIGVNSIEEAIRVRKVDDSTPVLVMGANDKADYKEIASYASKPVLVLSTI
                     DAIRELEDHLPGHPFHLKVDTGMSRLGHHGVSLQKIFSYLSAHPELNWCGLMTHFANV
                     EDVTDQTYAQEQLNRFDEASAFAVSAAGNRKLAFHTAASGAALLLPESRRDLVRVGIS
                     LYGMWPSRETRISASALTAQGTLDSSFKLEPALQWKTRIVHINAITAGTAVGYGCTYR
                     PVQDSNIAVLPVGYFEGYDRRLSNKAHVLISGKRAPVIGRVCMNMIMVDITHIADAKV
                     GSVATLIGTDEKESVTADDLADWMGTINYEVTTKIEPGIPRILVD"
     gene            7602..>8171
                     /locus_tag="PKK76_05860"
     CDS             7602..>8171
                     /locus_tag="PKK76_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNL68028.1"
                     /translation="MKLPSRMPRRLLLIPLDLMIAASSIYLANALRFDSLIPQSWPTY
                     PGAAEALNVLALVVATRGVCFVLMGLYRSLWAYASIPDLLLILKTTAVSSLCILAILL
                     FYNRLEGIPRTVLILDSVLVFLLLGFRSFSWRLFRDIYFVRRARGGRRTLLIGAGVTA
                     NRLVTDLRTNESLQIMPIGYLDDDPAKGGA"
BASE COUNT         1999 a         2066 c         2109 g         1997 t
ORIGIN      
        1 aagctgccga tcccagggga tctggaaaaa gaactcaatg gcgcggggtt gtctgcgaag
       61 atcttcgcag gcgtccaggg caatccggtg gagtctcagg taacggcagg agtcgcggtc
      121 tttcgcgcgc acaacgcaga ctccattgta gctttaggcg gcggagctgc catggatgtt
      181 gccaaagcaa tcacactcat ggcgcatcac ccgggcaaac tctttgacta tgaagatgtt
      241 cctggaacaa agccagtgga tcagacgatt cctttcttcg tcacggttcc aaccaccgct
      301 ggcacaggaa cagaagtggg tcgcagtact gtgatctctg atgacacaac gcatgcgaag
      361 aagatcatgt tttcgccgcg actgctcgct cgttgtgttt tcgcggatcc ggaactgacg
      421 ctcggtctgc ctgctcgtgt aacagccgct acagggatgg atgcgctcac tcatctggta
      481 gaagcgtatc ttgccaatga ttatcatcca atctgcgacg gcatcgctct ggaaggagtg
      541 aggctggtgg cgcaaagtct ggtgcagtgt acggaatatg cgaaagcaaa agacccaggc
      601 tcggatgatc acagacgcgt acgcggcatg atgctaaacg ccgctatgat gggtggaatc
      661 gcttttcaaa aggggctcgg tgccgttcat tcaacggctc atcctctttc cacggtcttt
      721 gattttcatc acggtcttgc aaacggaatc atgattcctt acgttatgcg ctttaacgcg
      781 gaggcagtgc cagagcgatt tgtcactctg gcccaggcag cgggaatcgc caatccaggc
      841 cccgactctt tcattcactg gcttgaaaat ctaaaagagc aacttggcat tccacgtacc
      901 atttctgagg ccggtgcgaa gaaagaaggt ctcgaagatc tggtgcgatt tgcagttctc
      961 gatgtctgtc atgcaatgaa tccgaggcct gtgagcgaag gcgactttag aaacatatac
     1021 acgaaggcat ttggatcgtg agtttgcgca ttggtgtttc cgcctgcttt tttcacgcag
     1081 acccgaagag acctatcttt aagggcaaaa cacttgtata tgcggaagca tccatgttgc
     1141 agtgggtcat gtcaggtggc gccattcccg taatgatccc tccaccttac gctggttttg
     1201 cgattaaaga ttttctcgcg gaagtagacg gagttcttct tcagggcgga tcggatgtga
     1261 gccctacgag ttacggcgaa actcctctca agccggaatg gaacggggac cgcgtacgcg
     1321 atgattatga gaaaagtatc attgaaacgg ccattgagat ggatcgccct gtacttggca
     1381 tttgtcgcgg gctacagatc atgaatgtag ctctgggtgg ttcgctttac caggatattc
     1441 gagagcaaaa ttcggcttct attgttcatc gcgactggga tatttatgac gatttgctcc
     1501 acagagtcag gatcgatccg tcttccaggc ttggggccat gtatggcgaa ggtgggatga
     1561 tcaattcaat tcaccatcag gggatcaagg atctctctcc ccaattgtct gcggaagcgt
     1621 ggtgcgtgga agacggcatc attgaagcag tgcgattaaa gtcgaacaaa gcatattgcc
     1681 tcgggattca atggcatcct gagttcattc gcaaagacca gaatttactc gatccggccc
     1741 ccattctgaa ggagtttctg acggaagtga gcaatcgaaa atgatcgatc tggcccggga
     1801 tttcgcgcgg cttactgaaa aactcaagaa ctcaaaagaa atcagtatac ctttgcagtc
     1861 tctggaaagt gaagaggaaa tctttgcaaa cggtctggtc accaaagctc ttgcatacat
     1921 tggtcagatt cgtctggagg gtacgcttca tctggcagtg gcagaactac tgagcaacgc
     1981 cgaaaaagca cttctgaaaa gaacattcgc agaattcttc gcgaacactg cggactacga
     2041 caatcctgat ttcctgaaat cctttcatcg caagctgcgc gaagatttgc gttctctgcg
     2101 tgccattctt aaaaaatcta aaccttccat tgaagtgaac atgaagatcc aggcgtccgg
     2161 tctgtctttg agcattctca cgggttctat gccttccctg caggaagatg agttcattca
     2221 tcgtgccatg gaggctggtc tgcgtgcgaa gtctctggca gatctagaag cgttgccgga
     2281 tacgatcgaa gcggaagggc gcggacttgc catatcggtt ttctctctta aatctgccgg
     2341 aatccccggg atcgtctacg cgctgcaagg cgatcgagcc tgtttttcga tttccgtccc
     2401 cgcacacagg ccagatgaga attttctttc gcgcatcggc tcagatttga ttggcgaaat
     2461 ccaatctctc cctgcattcc ctgatactac gcgaaaactc cttgagatgt gcaactcgcc
     2521 tgacacggac ttgaaaagca ttgccctgga aattggaaga gacgcggcga ttgccggcga
     2581 actcatccgc gtggccaatt caggtggatt cgcaggaggg cgtgtagcgg acattgcgga
     2641 ggcagcaaag attctgggca tgtccaacat tacgggctta ctcatgcgct ctgccgccta
     2701 cgccattctg gatgaacgct acggagtcac agaagagctg cttgcacatc cggttcgtgt
     2761 ggcggtgtat tcgcgcacac tcgctcgcaa gtacaaactg agtgcgatcg cagaccaggc
     2821 gtacgtggcc ggtctcctgc acgacattgg gaagattgtg ttggtccatc atatgaagca
     2881 gaccggaagc ctctcctccg tagagaaaac ctccaggttc agagatcggc gaagccaggt
     2941 gaacctggag gaaatcgcct gcggtgcgga tcacgccttg atcggaaagc tcctgggtga
     3001 aaaatggaat ttcccggatg aattatgcat ggccattgga atgcatcaca gtccactcgg
     3061 ggcgccaaaa gaaacgcgta agcttgcatt cgtggtgtac atggccaacg ctctggctga
     3121 ttatgaagag ggacagacag agttttattc tgtggaacct gaagtgctcg cgtttttcaa
     3181 tatcgcaaca gttcaggcct tccagatgct tgccggttca ctgaattcag agtacagcag
     3241 cacgtgacct ccgtgggcgg cacgtgaccg ggcgcaacgt aatatttcac tagccggcgt
     3301 gtcgtgtaac agggcgaagg tggattctct tcagcgcgtg gatcatcatg aacaacagaa
     3361 atccaggctc aggcttcaga aactgaacga gcgcacccgt cggcagttgt cgattcacat
     3421 tgttcaccca ccgaacggcg caccggaccc gaagggatgg tttgttgttt cttgaaatag
     3481 ataaatgaag tctgtctccc tgggaaagat cgtccacccc ggatagaaaa gctccacccg
     3541 gcagaatatc gaatatcatc gtatcctggg aagttcctcg ttcgtgctcg atatgcacca
     3601 gagtaccgga aggcactcga agcattgttt ctcgaaaata gaggtagttg atcttgtcgc
     3661 gaagcaacag tacagccgcc gcaaaggcag ggagtacaac tgttaaagca atctgcgcgt
     3721 gagcatcaat tccagaacca gagagccacg atggaaagcg aaaacggatt ataaagaaag
     3781 cggctgcgat cagaaaaaac aaaatgccca gagcagtggg cgtgatttcc cagcggttgc
     3841 gaaagatttc cactgcttcc ctgtcttcgt cctgatagtc gctcaaccat ttttcatatt
     3901 cttgctgcct gccaatgatt tccatgtcat ctgcatccag tccaagcgcc cccagaatgt
     3961 tttttccaaa aatctgcatg ccacgagctg ccatgtattc tgttacgata aatccagtga
     4021 ccagaatggg aacgagcatg tagttccaga catctccgcg aaacaacgcg tagtcgaaaa
     4081 aaccatgcag caaaaacgga atcaggaggg caaagactgc gagctcgaat cgtctgagcc
     4141 cgtaccagtt tagtctgcgc cagactttcc tgttttcgcg acagaggctt ctgtaagaaa
     4201 gaaaatatgc catgatccca ccgcaaaaca catgaagtgg aacagagcta atgcatcgca
     4261 gcaatatcac ataaccgctc gtagtcgcgc tgtagtgaag attttcggaa aacgcaaatc
     4321 caagggctac gagaataccg tcgaagattc cttcggtgac tgtaaatcct tccgaactct
     4381 ttcggatacg cagatagaga acagtgaacc ggattgcctc ttctaccagg gctgcgtgaa
     4441 aaaaagatcg aaccgccggg cttgcggtct cgggaagaag ggcctggata acaatggcgg
     4501 caatgctcga aagcattcca agaaagaacg ctttcgattg ccagagaata gatctttgaa
     4561 atacgaagtt acggaagtag agcgcgaaaa ataaaccaac cggaatggtg gatagtatga
     4621 ttcgcgctgc gtattccact ggaaaagagc gctctcgcgc tcatctcaaa attcaggccg
     4681 ctcgccgtct ctgaatcatt gcgagagcaa ggccaatcac tgcaatggca gcaatgagtc
     4741 cgagaatcat ccagcttgcc ggagacttca agtaagcaaa caggctgctc gcgttgaata
     4801 cgtgagagct gactgcactc gaataccagt ccttatttgt cgttccagaa gacagatacc
     4861 agggtcggaa tcctggataa ccgttctcga tgttgtctga ttcaagaggc cacttccatg
     4921 cttctggaat ctggagcgcc cacgggaatc cgtttgtgtc catgtattga tctttgccgt
     4981 tcgcatcaaa atacatgcga gggaaatgaa cgtcgaggcc cgtgttgttc acgtgaatgt
     5041 agagatcaaa cggagcgctg cccagagtac tttgcgaaac gggagtatca aacgtgattt
     5101 cgatgtctgc acgcttgcca ggagtgaatg tctgccccga agaagtgttg gactgagtga
     5161 ttgtgttgtt cgaatttccg agaatcgcaa gcccactacc attcgagtaa tgattggttg
     5221 tggtggactc aagtgcacct gctgcgctgt actgttttac agtatagttc agtgcgcctg
     5281 ccgctgcagg tagagtgagt ttcaattgat gggtgtaacc tgcaccgcga gcaatgtgcg
     5341 tataactacc gcgaaggcga accaccttcc ctgccgcatt caggtcttct tcattggaaa
     5401 cctttacaac gtagtcgttg aaatctgcat cacccggtga agggtagagg tcttcgtatg
     5461 caatgataga ctggccgctt ccaggaatag ttaccttcgt ggcgcgagtt ggatcggatg
     5521 gataggcatc atttgcatct gtaattccgt ctccgtcctg gtctccgccg gatgaaatgg
     5581 taacatcccc tggataatcc acgtctcgat cgcaggtgac gagattggtt gtattcacgg
     5641 gctgcgtgac tacattgccg cctgcatcta cgacgagtgt aacttctgtt acagtgttat
     5701 ccaccgtgaa cgttccgctc gcctggccgg tttcatttgt gactgcctga aaaataactg
     5761 tgttggccgc ggaaggatcc tgcacctgca ctacagcgcc ttgcaccggt ccgttcgaat
     5821 cggccacagt cacgctgacc ggaactgtgg ttgttgtctg aaatgaaaat ccagttgttc
     5881 cggtctggtc attgttcgtt gtagtgactg aatcacctgc agaggatgtc gtttccgcag
     5941 tctgcgcggt ttgattgccg agcagagcca tccaccacat ggccgctttc tttttcccgc
     6001 caccgcaatt cgcagcagcc aggacgatca taagacttgc taagtatttc acatcttgcc
     6061 cctttgcatg aacaacataa aacaggtaat gtacaacatc aataattttt gactgatccg
     6121 ttcgtgatcc gttccaggca gaaaggacta gactgcgtcg aagtcgattt caggaaaaat
     6181 gtctcagtcc acaaggatgc gtgggattcc cggttcaatc ttagtggtta cttcatagtt
     6241 gatagttccc atccaatccg ccagatcatc tgcagttaca gattctttct cgtctgtgcc
     6301 aatgagagtc gccacactgc cgacttttgc atctgcaatg tgcgttatgt ctaccatgat
     6361 catgttcatg cacactctgc cgatcacggg agctcttttc ccggaaatca aaacgtgagc
     6421 cttgttcgaa agtcgcctat cgtatccttc aaagtaccct acaggtagaa ctgcaatatt
     6481 tgaatcctga actggtctgt atgtgcatcc gtaacccact gccgttccag cggtaattgc
     6541 gtttatgtga acgatccttg ttttccattg aagagcgggt tccagcttaa acgaactatc
     6601 cagggttccc tgggcagtga gagcggacgc agagatgcgc gtttctcgcg aaggccacat
     6661 tccgtagagg gaaatcccga ctcgcacaag atctcgtctg gattcaggca agagaagagc
     6721 ggcgccactg gctgccgtat gaaacgcaag ctttcgattt cctgcggcag acaccgcaaa
     6781 tgcggacgct tcatcgaatc gatttaactg ttcctgcgcg tacgtctggt ctgttacatc
     6841 ctcgacgttt gcaaaatgag tcatcaatcc gcaccagttc aattcaggat gcgcggacag
     6901 gtaagagaag atcttctgaa gagaaacgcc gtggtgcccc aggcgagaca tgccagtgtc
     6961 tactttcaaa tgaaaaggat gtccaggaag gtgatcctct agttcgcgaa tggcgtctat
     7021 cgttgaaaga accagaacag gtttgctcgc gtagctcgca atttctttgt aatccgcctt
     7081 gtcgtttgcg cccatgacga gaacgggcgt tgagtcatcc accttccgaa cgcgtatggc
     7141 ttcttctatt gaattcactc caatccagtc tgcttggccc cggacagccc gtgtgcagag
     7201 ctccagatcg tgaccatatg cattggactt caagacggcc atgactcccg tcgatgtagc
     7261 gcggcgaaaa tggattagat ttgattttag cgcagatgcg cgtatttcta cgtacgtatg
     7321 catgaattta aatgcttgtt cgcctgggtg cccaatggaa tctagccaga aattccgctc
     7381 gtcggctaaa taaaatgaaa aagctcaaaa ctacccggtc cgttcggatc aaagtcgctg
     7441 aaaagcgtac aaagcgcgcc ctccatagca aagctaagag aacccaccgg atgagcaccg
     7501 gattcaagga aatcgaagaa aaatcagaat cctgatcgtt ggtcgtgaat tttcggccga
     7561 tgcaatgaaa aggacgcgtt ccttcgtctc ataggtggct catgaaactc ccatcgcgta
     7621 tgcctcgcag gctacttctg atccccctgg atctgatgat tgcggcctcc tccatctacc
     7681 tggccaatgc tcttcgattc gactctctca ttccacaatc gtggcctacc taccctggcg
     7741 ccgctgaagc gctgaatgtc ctggccctgg tcgtggctac gcggggggtc tgctttgttc
     7801 tgatggggct gtacaggagc ctctgggcat atgcttccat accagacctt ttgctcatcc
     7861 tgaagaccac ggccgtgtct tctctttgca tcctcgccat ccttctcttc tacaatagac
     7921 tcgaagggat tccgcgtacg gtgctgatac tcgattctgt acttgttttc ctgcttcttg
     7981 gcttccgtag ctttagctgg agactgtttc gcgacatata ctttgttcgt cgcgcgcgcg
     8041 gcggcaggcg caccctgctc atcggagccg gagtgacggc aaatcgactg gttacggacc
     8101 tgagaaccaa cgagtcgctg caaatcatgc cgattggcta tctagacgat gacccggcca
     8161 aaggcggggc g
//
LOCUS       DAORFF010000103         8128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4316_length_8128_cov_3.753499, whole genome shotgun sequence.
ACCESSION   DAORFF010000103 DAORFF010000000
VERSION     DAORFF010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4316_length_8128_cov_3.753499"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3140)
                     /locus_tag="PKK76_05865"
     CDS             complement(<1..3140)
                     /locus_tag="PKK76_05865"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL68029.1"
                     /translation="MEALDFLNRHSIYLNYFAIGAIICFIFNFICGAFLLALPHKTKS
                     TTHMGIGLMFMSFFVFGYVISQSFYSDIAAYHRWVTVGFVLPAIVHFVQWLLRFPENN
                     KPGLTRWTGVAMWALSLGALALFMYASVGADRKFHFSAHYWDFDLEKASKYVGFLIFA
                     FVLCMPGFGIWKFVIVKTKERYAIVLIIASLLIASVIPAILNIQSRDGVVERGTFLLV
                     FVLTIVGGFFMVILTYMNTTKDRSTFMAKIVGITLVTFLVMMQGVSLVTMTDREEEYD
                     ALRVQYMLRALDGADVHPTIEYIVALEPVKKTIDQSYQSERYAQLPESKRVDFVAQTP
                     ALLNTRIFEEIQSASAQPQFRDAVANIVRDSHVEFAGYKAALEDAAKQAPNDDALKGS
                     INDYMARLDKEAFINTNRISGIPSDSHFREELMKHLGKANKYFAFFVPGIQEYMKQNP
                     DMPPVTLKKGILKYLARFSPDHTRVYCQSPDGNQHFVAFNVYDAGTGTMHEVGYSYQE
                     YRAFVHPAAKRLVFILLIVLVVLVVLYPLFFRGSLVNPLNSLLRGVTKVNQGDLTVEV
                     PVKVQDEIGFLTTSFNSMVASIKDARAKLEDYAANLEEKVQARTAELNKTLEVVHALK
                     IQQDGDYFLTSLLAKPLFFNANKSRSVTTEFLLRQKKHFEFRNKQSELGGDICITGNL
                     RFSKHPERRYVVSLNGDAMGKSMQGAGGSLVMGVVMNSILARSAKNNRVLTISPEQWL
                     KDVYEEVHSVFLAFNGSMVISVVMSIVDEVTGEMWYWNAEHPFQILYRNGKASFIEED
                     LQLRKVGLESEIPFRVRNFQLQPGDVVLMGSDGRDDIDLTPDASTRTINEDEFLILKR
                     VEESRADLNTILERLLATGELTDDLSFLRIEFKGVTAAGTSGPTESKPVAMAAPERVI
                     IDIDPDDEDDMNFEDVEELGSFEELFQEGRRLARQGKHEEALELLRQAYAMRRDVPAL
                     NKILAVLTFKDRDYPKAIEILGNYLEHDPHVADFWLYLSIAHKRMGEYERSLEAAQKV
                     YEINSNRIPNLINMADL"
     gene            complement(3224..4132)
                     /gene="lpxC"
                     /locus_tag="PKK76_05870"
     CDS             complement(3224..4132)
                     /gene="lpxC"
                     /locus_tag="PKK76_05870"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNL68030.1"
                     /translation="MTVSATHPGLTKRATVRGIVHIDGIGLHSGEAANLRIKPAPAGT
                     GLVFQSPNHRGPIEVTPFNAVDTRQAVTLSNKNWKVQTVEHLLAALAATGVTDAILEL
                     DSQEVPILDGSSRIFVQEIQKVGVRELGAELEPIRLAQAVWVVDGDRYIVALPSDSFR
                     VTCTIDFPHPELRGQSISMDLDSRTFLEEVVDARTFGFLTDVEALRKKGLIKGASADN
                     AVVLTEDGYLDREGLRFSDECIRHKVLDLVGDLYLMGRPIHAHVIAHRGGHGLDVALA
                     RLILQRTAMDELSARRQQKADTVVAL"
     gene            complement(4339..4914)
                     /locus_tag="PKK76_05875"
     CDS             complement(4339..4914)
                     /locus_tag="PKK76_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68031.1"
                     /translation="MGFLQKFFRKKDDPDLFEYAARSQEEEPSKKRRFWFLLIPIVIP
                     ASFFAGFQLGKDNSCEEERATISAMADTGTTNPQTAPYQSFLGTYKVDVSGHQGLLYI
                     YSLPSGGPGATIHFQNWGRRVPEPLFAVQTAGNRITFVRACQAQRCQEIGAPGPFRQD
                     YQGTLSDDAKKLEGTYTGGQSASTWRAIRIR"
     gene            4995..6203
                     /locus_tag="PKK76_05880"
     CDS             4995..6203
                     /locus_tag="PKK76_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL68032.1"
                     /translation="MSPEAHERSILLILAGMQFTNILDFVIMMPMSATFIQVFHIEPA
                     QFAILLSSYSTSAAIAGVLSAFFVDRFDRKTAVLIAYGGFLVGTLLCALSQEYSQLMA
                     ARVLAGAFGGVLGALVLSMVSDAVPESRRGQAVGIIMASFSVATIAGVPAGLLLNEVL
                     NWHVPFYFITAIGAIIWPIAFLHLPAFRGHIAPVKRSALETLKSMGSMITHGSHLRAM
                     VLTVLLMFSGFTVIPYIAPYLTYNVGLEKKYLALVYLCGGLATIFSSRYAGRLADRHG
                     KQKVFSIIGLFSIIPIVALTSLPPLPMAAILGVTTIFMIFVNGRFVPAMAMITTSARP
                     KDRGSFMSVQSSVQHLFSGVSAAIGGALLTKGPEHTLIHYEWVGYFASAMTIVAIAWV
                     WTLKSQETPR"
     gene            6170..6844
                     /gene="nth"
                     /locus_tag="PKK76_05885"
     CDS             6170..6844
                     /gene="nth"
                     /locus_tag="PKK76_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNL68033.1"
                     /translation="MDFEITGDAALKQRDDAEEFVKNAYKLLQKKFGDVKCPLTYGKP
                     HELCIAVILSAQCTDAQVNKTTPALFARFPNPGDYISAPLSEVENLVHSTGFYRNKAK
                     NIQGFCHSLVHEHGGVVPRTLAELTAMPGVGRKTANVVLQELYGIPSGMVVDTHVARL
                     SRIFGLTKSKNAVQIERDLMQIVPERMWMNWSLYMIFLGRSNCTARTRDCATCILKKI
                     CPAANY"
     gene            complement(6838..7257)
                     /locus_tag="PKK76_05890"
     CDS             complement(6838..7257)
                     /locus_tag="PKK76_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68034.1"
                     /translation="MSQSEQKETIEELAASLEKISQKKLKYDIVRHWFQGREPYFDLF
                     VNTLNDTLVWFQFTFRGHSICWSQQDPIIRTGVTTDFALQDVSYYSATKTVKLDEEID
                     PQFLAFCKKLAGCRATEEPFRSILKVLSSPPDLTANQ"
     gene            7337..>8128
                     /locus_tag="PKK76_05895"
     CDS             7337..>8128
                     /locus_tag="PKK76_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNL68035.1"
                     /translation="MQDHLFSPAAPLPQRLRPSSFEEFHGQQSLIDQLAGKKPHSMIL
                     YGPPGCGKTTLALLLAKKATLPFRSLSAVASGVKELRDVIEEARKTGRILLFVDEIHR
                     FSKSQQDALLHAVEEGWLVLIGATTENPSFEVISPLLSRCQVYKLGALSSEDLDRILE
                     RALMQDSLLKGKTIEGEAREALIQFAGGDARKLLRVVEIAAEEADIITLKALNKSIQN
                     FARQYDRAGENHYDVISAFIKSMRGSDPDAALLYLAIMIDAGEDPL"
BASE COUNT         2016 a         2048 c         2045 g         2019 t
ORIGIN      
        1 agatccgcca tattgatcaa gtttggaatc cggttcgagt tgatttcgta taccttctgg
       61 gcggcctcaa gggatcgctc atattcgccc attcgtttgt gcgcgatgga gagataaagc
      121 cagaaatcag caacgtgcgg atcgtgttcc aggtaattgc ccaggatttc tattgccttg
      181 ggataatcgc gatccttgaa agtgagcaca gcaagaatct tattcagagc aggaacatct
      241 cttcgcatcg cgtatgcttg tctgagcaac tccagcgctt cttcgtgctt tccctgacgt
      301 gcgagccttc taccttcctg gaaaagttct tcgaaactgc ccagttcctc tacgtcttcg
      361 aagttcatgt catcttcgtc gtccgggtca atgtcgatga tcactctctc cggcgcagcc
      421 atggctaccg gtttggattc agtcggacct gaagttccgg ctgcagtcac gcctttgaat
      481 tcaatgcgaa ggaaggaaag gtcgtctgtc aattcgcccg ttgcgaggag tctttccaga
      541 atcgtattca aatccgcgcg agattcttct actctcttca agattaagaa ttcgtcttcg
      601 ttgatcgttc gtgtggatgc atctggagta aggtcaatgt cgtcgcgacc gtccgatccc
      661 atcaaaacca cgtcacctgg ttgaagctgg aaattccgaa cgcggaatgg aatttctgac
      721 tcaagaccta cttttcgcaa ctggaggtct tcttcaatga aggaggcttt tccatttcta
      781 tagagaatct ggaaaggatg ctccgcgttc cagtaccaca tttcgcctgt cacttcatcc
      841 acgatggaca tgacgaccga gatcaccatc gatccgttga atgcaaggaa gacggaatga
      901 acttcttcgt acacatcttt caaccattgc tctggagaaa tcgtaagtac acgattgttc
      961 ttcgcggaac gagccaggat ggagttcatc acaacaccca tcacgaggga tccaccggca
     1021 ccctgcatgg atttacccat cgcatctccg ttcagcgaaa ctacatatct acgctccgga
     1081 tgcttggaga agcgaaggtt accggtgatg caaatatcac cacccagttc cgattgcttg
     1141 tttctgaatt caaagtgctt cttttgacga agcagaaatt cggtggtcac ggagcgagat
     1201 ttgtttgcat tgaagaaaag cggttttgca agaagagaag tgaggaagta gtccccatcc
     1261 tgctggatct tcagtgcatg caccacttcc agggtcttgt tcaattctgc cgtgcgagcc
     1321 tgaactttct cctccaggtt ggcggcgtag tcttctagct tggcgcgcgc atccttgatg
     1381 gatgcaacca tggagttgaa agacgtagtg agaaagccga tttcatcctg aactttgacc
     1441 ggcacttcta ctgtcagatc gccctgattc accttggtca caccacgcag aagtgaattg
     1501 agtggattga caagactccc tctgaagaac agaggataga ggacgaccag aacaaccaga
     1561 acgatcaaca aaataaagac gaggcgtttg gccgcaggat gaacgaatgc gcgatactcc
     1621 tgataggaat atccaacttc gtgcatcgtt ccggtgcctg catcgtacac gttgaaagct
     1681 acaaaatgct gatttccatc cgggctctgg caatagaccc tggtgtgatc cggactgaat
     1741 cgcgcgaggt atttaagaat tcccttcttg agtgtcaccg gtggcatatc cggattttgt
     1801 ttcatgtatt cctgaatgcc cggaacaaag aatgcaaagt acttgtttgc cttgcccaga
     1861 tgtttcatca gctcttcgcg aaagtgacta tcggaaggaa tgcctgagat tcggtttgtg
     1921 ttgatgaacg cctctttgtc caggcgagcc atatagtcat tgatagagcc tttgagcgcg
     1981 tcatcattgg gcgcttgctt tgctgcgtcc tcgagagccg ctttgtaacc ggcaaactca
     2041 acgtggctat cacgcacaat attcgcaacg gcatcgcgga actgaggctg tgcagaggcg
     2101 gactgtatct cttcgaaaat acgagtgttc agcagggccg gtgtttgcgc tacaaaatcg
     2161 acacgcttgc tttccggcaa ttgcgcataa cgttccgatt gatacgactg atcgatggtc
     2221 tttttcacag gctcaagggc cacaatgtat tcgatcgttg gatgaacatc tgctccatcg
     2281 agtgcgcgaa gcatgtattg aactcgtaac gcatcgtatt cctcttcgcg atccgtcatg
     2341 gtaacgagac tcacgccctg catcatcacg aggaacgtca caagagtgat accaacgatc
     2401 ttggccatga atgtggaacg atctttggtc gtgttcatgt aggtgaggat caccatgaag
     2461 aaaccgccaa cgattgttaa cacaaatacg agaagaaacg tcccgcgttc cactacacca
     2521 tcgcggcttt gaatgttgag aatcgcagga atgaccgacg caatcaggag tgatgcgatg
     2581 atcaacacga tcgcataacg ttcctttgtc tttacgatca cgaacttcca gataccaaat
     2641 ccgggcatgc acagaacaaa ggcaaaaatc aggaatccaa catacttgct ggccttttcc
     2701 agatcaaagt cccagtagtg agcagagaag tgaaacttac gatctgcacc tacgctcgca
     2761 tacatgaaga gagccagagc accgagggaa agagcccaca tggccacccc ggtccagcga
     2821 gtcagacctg gtttgttgtt ttcaggaaag cgaaggagcc attgcacaaa gtgaacaata
     2881 gctggaagaa caaagcctac agtcacccag cgatggtaag cagcgatatc gctatagaaa
     2941 gactgactga ttacatagcc aaaaacaaag aaggacatga acatgagtcc aatgcccatg
     3001 tgcgttgtgc tttttgtctt gtgtgggagc gctaagagga aagctccaca aatgaagtta
     3061 aagatgaagc agatgatcgc gccaatggcg aagtagttca ggtaaatact gtggcgattg
     3121 agaaaatcta aggcttccaa cgcaactccc catggaccgg gaacggccga tccatcattt
     3181 atagttcggc aaatcgggga gtgtcaacag tatttttaga ggcctacaga gccacaactg
     3241 tgtctgcttt ttgctgtctt cttgctgaaa gttcgtccat tgcagtgcgc tggaggatca
     3301 gacgagccag agcaacatcc aatccatggc cgcccctgtg cgcaattaca tgagcgtgaa
     3361 tggggcgtcc catgagatac agatcgccta cgaggtccag gaccttatgg cgaatgcatt
     3421 cgtctgaaaa gcgaagacct tcgcggtcca ggtaaccatc ttctgtgagc acaactgcgt
     3481 tgtctgcgga agcacctttg atgagtccct tcttgcggag agcctccaca tctgtcaaaa
     3541 agccaaaagt gcgcgcatcc actacttctt ccaggaacgt tctgctgtcc agatccatgc
     3601 tgatggactg accacgtagc tccggatgcg gaaaatcaat ggtacatgta acgcgaaatg
     3661 agtcagaagg aagggctaca atataacgat ctccgtccac tacccagact gcctgtgcaa
     3721 ggcgaatcgg ctccagttcg gctccaagct ctctaacgcc tactttttgg atttcctgca
     3781 cgaagatacg cgaagagccg tccagaatag gaacttcctg ggaatcgagc tcgaggatcg
     3841 catctgtcac gccagtggct gcaagggccg cgagaagatg ctctaccgtc tgaactttcc
     3901 aatttttatt gctcagagta acagcctgac gagtgtccac agcattgaaa ggggtcactt
     3961 cgattgggcc acggtgattg gggctttgaa aaacaagacc ggtgccggct ggagctggtt
     4021 tgatgcgcaa attggcggct tcacctgaat gcaggccgat gccgtcgatg tggacgatgc
     4081 cgcgcacagt ggcacgtttc gtcagaccgg gatgtgttgc agatacagtc ataggcttct
     4141 gcccccctaa cgcaagaccc gtaccagggg agccctttca caagaaacgc cgcgatttgg
     4201 ccattctgtg cagattttga cggcgagacc gcaaatcggc cctggccagc cattgcaggg
     4261 aatgagacat aatctgtccc gattgtagta agggcgcact cagtgatgcg gttctgcaac
     4321 acgaccctgg acaggcttct accggattct aatcgccctc caggtgctgg ccgactgccc
     4381 gcctgtgtat gttccttcca gctttttggc atcatctgag agagttccct gataatcctg
     4441 cctgaacggc cctggagcgc caatctcctg gcatctctgc gcctggcagg cacgcacaaa
     4501 tgtgatccga ttgccggctg tttggactgc gaaaaggggt tctggaacac gacgccccca
     4561 gttctgaaaa tgaatcgtgg ctccaggacc accggaaggg agagagtata tatagaggag
     4621 accctgatgt ccgctgacgt ctaccttgta ggttccgaga aacgactggt aaggcgctgt
     4681 ttgtggattg gtcgtgccag tgtcggccat cgcactgatg gtcgctcgtt cttcttcgca
     4741 actgttgtct ttaccaagct ggaacccggc aaaaaaagaa gccggaatta caattggaat
     4801 caacaggaac cagaatcgcc gctttttgct tggttcctct tcttgagagc gtgcggcata
     4861 ctcgaacagg tctggatcat ccttcttgcg gaagaatttt tggagaaagc ccatgtatct
     4921 tctcatcggc tcaaaagaga acagattgag ccttatgttc gtgactggaa acgcaatccc
     4981 gcgagtttga cccgatgtct cccgaagcac acgaaagatc catcctgctg atccttgcag
     5041 gcatgcaatt tacgaacatc ctggatttcg tgatcatgat gcccatgagc gctacgttca
     5101 tccaggtttt tcacattgag ccggcgcaat tcgccattct actgagttcc tattctacga
     5161 gcgccgccat cgcaggagta ctgagcgcat ttttcgtgga tcgattcgac agaaagactg
     5221 cagtgctcat tgcctacggt ggattcctgg ttggaactct actctgcgcg ctttcgcaag
     5281 aatactcgca gctgatggcc gctcgcgttc tcgccggtgc atttggaggt gttctgggag
     5341 ccctcgtgct ttccatggtc agcgacgccg ttccagaatc tcgtcgagga caggccgtgg
     5401 gtatcatcat ggcttctttt tcagtggcga ccatcgctgg agttccggct ggattgcttc
     5461 tgaacgaagt tctcaactgg cacgttccgt tttatttcat cacagcgatc ggcgcgatca
     5521 tctggcccat cgctttttta catctccctg cttttcgcgg tcacatagca ccggttaaac
     5581 gatctgcgct cgagactctc aagtcgatgg gctccatgat cacgcatggt tctcaccttc
     5641 gcgccatggt gttgacggta cttcttatgt ttagcggctt cacagtgatc ccatacattg
     5701 ctccttatct cacttacaat gtaggactgg aaaaaaaata tctggctctg gtctatttgt
     5761 gcggaggcct cgcaacgatc ttctcttctc gctacgcagg aagactcgcg gatagacatg
     5821 gaaaacaaaa agttttctcc atcattggtc tgttctccat cattcccatt gtagcgctca
     5881 cgagtctgcc ccctcttccg atggccgcca ttcttggtgt gactacaata ttcatgatct
     5941 ttgtgaacgg tagatttgtt ccggccatgg caatgatcac aaccagtgct agaccaaaag
     6001 atcgaggctc gttcatgagc gttcaatcct ctgtgcagca tttgttttcg ggagtatctg
     6061 cagcaattgg cggtgcactc ctcacaaaag gtcccgagca cacactgatt cattatgaat
     6121 gggtgggcta ctttgcatcc gccatgacca tcgttgcaat tgcctgggtg tggactttga
     6181 aatcacagga gacgccgcgc tgaaacaacg cgatgatgca gaagaatttg taaagaacgc
     6241 ttacaaattg ctccagaaaa agttcgggga tgtaaaatgt ccgctgacct atgggaaacc
     6301 tcacgaactg tgcatcgcgg taatcttgag cgcacagtgt acggatgctc aggtaaacaa
     6361 gactacgccg gcgctcttcg cgcgcttccc aaatcctggg gactacatat ctgcgccttt
     6421 gagtgaagtc gagaacctgg ttcacagcac tggtttctat agaaacaaag cgaagaatat
     6481 ccagggattt tgccattcac tcgttcacga gcatggaggg gttgttcctc gcactctcgc
     6541 agagctcact gcaatgcccg gggtcggcag gaaaacagca aacgtagttc ttcaagaatt
     6601 gtatgggatc cccagcggga tggttgtgga tactcatgta gcgcggctgt ctcgaatctt
     6661 cggacttacg aaaagcaaaa acgctgtgca aatcgaacga gacctgatgc aaatcgttcc
     6721 agagaggatg tggatgaatt ggtctctcta catgatcttt ctggggcgat cgaattgcac
     6781 tgctcgcacg cgcgactgcg cgacctgcat tttaaagaag atctgcccgg ccgcgaacta
     6841 ttgatttgcc gttaaatcag gaggagagga cagaactttc agaatggatc ggaatggttc
     6901 ttccgtcgcc ctgcaacctg cgagtttttt gcagaaagct aaaaactgcg gatcgatttc
     6961 ctcatcgagt ttcacagtct ttgtagctga atagtaagaa acgtcctgga gcgcaaagtc
     7021 cgtagtcact ccagtgcgaa tgattggatc ctgctggctc caacaaatgg aatgacccct
     7081 gaaggtgaac tgaaaccaga cgagagtgtc attgagcgta ttcacgaaca gatcaaaata
     7141 gggctcgcgg ccctggaacc agtggcggac aatatcatac ttcagttttt tctgactgat
     7201 tttctcaaga gaggccgcca gctcttctat ggtttctttt tgttctgact gactcattct
     7261 gctcgccagg attagcctgg atggtcgtat gacgttaaaa atatcggtaa atagcgcaat
     7321 tcatatttag tagaacatgc aggatcatct tttttcaccg gccgcgccac ttcctcagcg
     7381 actgcggcca tctagttttg aagaattcca cggccagcaa tctctcatag accagctcgc
     7441 cgggaaaaaa ccacactcga tgatccttta cggacctcca ggatgcggga agacaactct
     7501 tgctttgctc ctcgccaaaa aagcaacgct gccgttcaga agcctgtccg cggtggcgtc
     7561 tggtgtcaaa gaattgagag atgtgatcga agaggccagg aagaccggaa gaattctact
     7621 gtttgtagat gaaatccaca gattcagcaa gtcacaacag gatgctcttc tgcatgcagt
     7681 ggaagaaggc tggctggtat tgattggagc caccacagag aatccgtcgt ttgaagtaat
     7741 ttctcctctg ctttctcgct gccaggtcta caaactggga gcgctttcga gtgaagacct
     7801 ggatcggatc ctggagcgag cccttatgca ggattcattg ctcaaaggaa aaactataga
     7861 gggagaggca agggaagctc tcattcaatt tgcgggaggg gatgctcgca agctacttcg
     7921 agtggttgaa atagcggcag aagaggcaga catcatcact ctcaaggctc tgaacaaatc
     7981 catacagaac tttgcgaggc aatatgatcg cgcgggtgaa aaccattacg acgtaatcag
     8041 cgctttcatc aaatccatgc gcggttcgga tccagacgcg gcgctcctct atctggcgat
     8101 catgatcgat gctggtgagg atcctctt
//
LOCUS       DAORFF010000104         8077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4359_length_8077_cov_3.862877, whole genome shotgun sequence.
ACCESSION   DAORFF010000104 DAORFF010000000
VERSION     DAORFF010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4359_length_8077_cov_3.862877"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PKK76_05900"
     CDS             <1..888
                     /locus_tag="PKK76_05900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNL68036.1"
                     /translation="NRARHALRRNTRARSKKNISEHYDLSNEFFSLLLDKSMTYSCAM
                     FTKELSLHQAQLRKFEVICQKLGLKEGMRVLEIGCGWGGFAVHAATHFGVHMDCITIS
                     REQHDWVKKLISERGLEDRITVRMLDYRDLTGQYDRVVSIEMVEALGYEFLDQFFQKA
                     ASALAPDGLMLIQAITFPDPYYEPYLQQTDWTQKYIFPGSCLLSLRETLNAIHRTSDL
                     VLWDVESLGPNYAETLRQWRKNLHTNRSAIEKLGYDERFFRKWNYYLVFCEVGFEARY
                     INVQQWLFGRPMAQSLRSI"
     gene            898..1623
                     /locus_tag="PKK76_05905"
     CDS             898..1623
                     /locus_tag="PKK76_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68037.1"
                     /translation="MKKNILRFLVLLIAGGPLHAVEKRIEYFAKAYDRNTGKFIYTEM
                     HSEIWVGGKHAGSEINYLGPDGKVLASKTIRYGSRKAAPDFKFEDKRTGYLEAAYWSG
                     QSSMEFWKRENPGDQVRQVRLNAPTSDTVVDAGFDYFIRENWERLMQQDVLVHFAVPA
                     RHSFFDFRIQRANRPASNPALVLFDVRIANPLLNLLVDKITVGYDRNSRRLMTYEGLS
                     NISDENGKSRAARIVFDYSKAGL"
     gene            1623..2705
                     /locus_tag="PKK76_05910"
     CDS             1623..2705
                     /locus_tag="PKK76_05910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNL68038.1"
                     /translation="MDSEQTIQSEAQGPGALDRLLAHGLVPDFLIRSGIRRLNRVRLK
                     EEFAANHEVSQKRQQELIARIRKSPLAVSTREANEQHYEVPPEFFELVLGKHLKYSSA
                     YWGEGDKTLEEAEERMLKLTCQRAQLEGAKSILELGCGWGSLTLYMAEHFPSAQITAV
                     SNSQPQRLFIEARARQRGLKNITIITSDMNSFEPSDTFDRIVSVEMFEHMRNYEILFD
                     RLSKWLNLGGKLFVHIFTHKHISYLFEPRDSSDWMSRYFFTGGIMPSNHLLLYFAQGF
                     TISDHWIVNGQHYQKTSEAWLKNMDNNLHRIRRLFDETYGAESRKMIEYWRIFFMAVA
                     ELFGTKKGEEWMVNHYLFEKTGNRGS"
     gene            complement(2713..3876)
                     /locus_tag="PKK76_05915"
     CDS             complement(2713..3876)
                     /locus_tag="PKK76_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNL68039.1"
                     /translation="MTLAGDKVRFEFATSTRIVFGPGVFREIASDIRLLGKTAVIVHG
                     KSRERAQALAGLLEMPVVECEIAGEPRIDQIRAVMRDLHNVREPVVIGLGGGGVLDGA
                     RAVAGLLTNPGDILDYLEIIGKGKTIAHRAAPLVAIPTTSGTGSEVTRNAVLTSPEHS
                     LKVSLRSPLLLPLIACVDPELTLTMPPALTANTGMDALSHILESFVSHANNPMTDAIC
                     REGLMRARSLLPAFKNGNDLAARSDMSLASLMGGMALANSRLGAVHGFAAPLGGTFDA
                     PHGAVCAALLAPVMEVNIRALRGRDPGSPSLERYAVVAQILTNDQSVTAEDGVKWVSD
                     LAHHLGISGISSFGVTDSHFSELCVKAAAASSMKGNPIVLQTEELQEILHRAR"
     gene            complement(3857..4165)
                     /locus_tag="PKK76_05920"
     CDS             complement(3857..4165)
                     /locus_tag="PKK76_05920"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNL68040.1"
                     /translation="MFIVQVSVHVKENAIESFRIASLENARMSVQEPGIARFDVLQNR
                     EDPSRFLLIEVYRKESDAADHKQTEHYKKWRDTVESMMAEPRQSTKYSNSFPDDSGWG
                     "
     gene            complement(4175..5215)
                     /locus_tag="PKK76_05925"
     CDS             complement(4175..5215)
                     /locus_tag="PKK76_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HNL68041.1"
                     /translation="MGAIWYLATFVLMGTCYGAFFLQGPWVWAPVVLVFGIVPILETL
                     PFLSRDVNNPTDERTLGQSTIFSDLLNAAVIFQCGLIGMLLIEASRSQNLLQLSGLII
                     STGLACGVYGINVGHELGHRADSFSQRMARLSLATSLYAHFFVEHNLGHHRNVATELD
                     PASSRRGEWLYTFWIRSIVMGYISAWKISAKRTRDRGHAFISIHNTMLVDQTLQAVFL
                     VGIALFLGTLPLLAFLLSALIGILLLETVNYIEHYGLSRQRTGDRYERVRPQHSWNSN
                     HIIGRLFLFNLPRHSDHHAWASRKYQILRHFDDSPQLPAGYPAMLMLAVLPPLWIPIM
                     NARVDSLDHVVS"
     gene            complement(5243..6580)
                     /locus_tag="PKK76_05930"
     CDS             complement(5243..6580)
                     /locus_tag="PKK76_05930"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNL68042.1"
                     /translation="MARLGLLLLVLFTHCYTSEEPVRTVRPVHVDEEKIRSLSYDLEA
                     YIQYHIAKGVFYSMTVGVVRGDDLVYSRYINSVPSRLYSVGSITKVFTATAAMRLQEK
                     GIIDFDERLSDVFPGLHVEQEMYHSPAVTLRHLLSHSSGMPDLRYYVPPEMIHHPEID
                     FPISPQTAPAGVQFRYSNLGYQLLGEAFKKISHRQISDIIDQEVYAAAGMNDSRMLPG
                     STGAYGVMTNVTDLAKFASVFLRRGLRKDARFLRAQSVDAMLRNQIYAPPAEFQDYNG
                     IGWRVRTDGQGIVQMYHVGGANGVAAWLQIFPRSNVALFYLGDPPKYEEDTMGVLVGI
                     QARLGNLATEFAGETRPLFVFRQSVSPETELPRFAGEYREPASGEVASVLLQGGGIWF
                     QKGISPPYPVVPECRAVFDGGYGNVQHAFILDQKDGHVLGVATNNGYYTKVTR"
     gene            6742..7083
                     /locus_tag="PKK76_05935"
     CDS             6742..7083
                     /locus_tag="PKK76_05935"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNL68043.1"
                     /translation="MDISILDMPSTEKLSPAALEILLILAGQSMHGYAIMKTSRGEGG
                     VRLGPGTLYRWLDSLSQTGYIRIKKEDGRRNLYEITPSGRKVLRDELSHAQNLLARAK
                     VAGIRTQGSGG"
     gene            7087..7569
                     /locus_tag="PKK76_05940"
     CDS             7087..7569
                     /locus_tag="PKK76_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68044.1"
                     /translation="MKPRKHQGRITLRRQLFLHVFYPEEFLKTHLESMCEDLSDLESE
                     GALQARDLIDLALNGLKIRIKHIARRMRPYHHALLTATVAFPLFAAFSAISFLFLPIN
                     LLPWILDFPFQDSLLMRAAIVLSLVSSILLIASERSPKNFLCLCINAPVTLFWTVLLF
                     "
     gene            7586..>8077
                     /locus_tag="PKK76_05945"
     CDS             7586..>8077
                     /locus_tag="PKK76_05945"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNL68045.1"
                     /translation="MKIALILLSVAFVFIAGFLAYSGALSTVVVKEEKVGPFHIVYRE
                     VKGSYRQTGKVSMEIQSLLKENGIDNDRFFAVYLDDPRSVPEENLRSLVGHILPPGSR
                     EAKLPAEYKSAILPERLALVSVFPLKTQASIFLGMFKVYPAIFAQAAKSGYPDKKPSL
                     EIYE"
BASE COUNT         2132 a         2095 c         1975 g         1875 t
ORIGIN      
        1 aatcgggctc gtcacgctct cagaagaaat accagagctc gcagtaagaa aaacatttca
       61 gaacactacg atttgtccaa tgaattcttt agcctgctcc tggacaaaag catgacctac
      121 tcttgcgcca tgtttacaaa agaattgtcc cttcaccagg cacagcttcg caaattcgaa
      181 gtcatttgcc agaagctcgg actaaaagaa ggaatgcgcg tccttgaaat tggatgtgga
      241 tggggtggat ttgctgtcca tgcggccact catttcggcg tgcacatgga ttgcattacg
      301 atctctcgcg aacaacacga ctgggtcaag aaactgatca gcgaacgtgg cctggaagat
      361 cgcataacgg ttcgaatgct cgactaccgc gacctcacag ggcaatatga tcgggtggtt
      421 tccattgaaa tggtggaagc tcttggttac gaattcctgg accagttctt ccagaaagca
      481 gcgtctgcac tggcaccgga tggcctgatg ctcattcagg cgatcacatt cccggatcca
      541 tactacgagc cttatctaca acagaccgat tggacgcaga agtatatttt ccctggatcc
      601 tgcctcctca gtctccgcga aacgttaaac gccattcatc gcaccagtga tctggtgctg
      661 tgggatgtag aaagccttgg gccaaactac gctgagacgc ttcgtcagtg gagaaagaat
      721 ctgcacacga atcgtagcgc gatcgaaaag ctcggatacg acgaacgatt cttccgcaaa
      781 tggaattact acctggtatt ctgtgaagtt ggttttgaag ctagatacat caacgttcag
      841 cagtggctct ttggccggcc tatggctcag tccttacgtt cgatttagga gatggatatg
      901 aaaaagaaca ttctaagatt cctggtttta ctcatcgcag gaggaccttt gcacgctgtg
      961 gaaaaaagaa tcgaatactt tgcaaaagca tatgatcgaa ataccggcaa gttcatctac
     1021 acggaaatgc actccgaaat ctgggtaggc gggaagcatg cgggttctga gatcaattac
     1081 ctgggtcccg acggaaaggt gctcgcatct aaaaccattc gctacgggtc gcgaaaggcc
     1141 gctcccgatt tcaaatttga agacaagaga accgggtatc tggaagccgc ttactggtct
     1201 ggacaatcgt ctatggaatt ctggaaacga gaaaatccag gcgatcaggt cagacaggtt
     1261 cgcctcaacg caccgaccag tgacacggtt gtggacgccg gttttgatta tttcattcgc
     1321 gaaaactggg aacgcctgat gcaacaggac gtgcttgttc atttcgcagt cccggctcgc
     1381 cacagcttct tcgattttcg catccagcgc gcgaatcgtc cggcatccaa tccggctctc
     1441 gtgttgtttg atgtccgcat cgcaaatcca ctgctcaatc ttctagtgga caagatcaca
     1501 gtgggctatg acagaaactc gcgcagactc atgacttacg aaggtctgtc caacatcagt
     1561 gacgaaaacg gaaagagtcg cgcagcgcgc atcgtgtttg attattcaaa ggcaggacta
     1621 taatggattc agaacaaaca attcaatcag aggcacaggg cccgggtgca ctggatcgac
     1681 tactggctca tgggctggtc cctgacttcc tgattcgatc cggcattcgc aggctcaatc
     1741 gcgtgagact gaaagaggaa ttcgcagcaa atcatgaggt cagccagaaa cgccagcaag
     1801 agctcatcgc tcggattagg aagagcccgc ttgcagtaag cacacgcgaa gcaaacgaac
     1861 aacactacga agtcccaccg gaattctttg aactggttct gggaaagcat ctcaaataca
     1921 gctccgccta ctggggcgaa ggcgacaaga ctctggagga agcggaagaa aggatgctga
     1981 aactcacctg ccagcgcgct cagctggaag gagccaaaag cattctcgaa ctcggttgcg
     2041 gatggggctc tctgacgttg tacatggcag aacattttcc atctgctcag atcactgctg
     2101 tttccaattc acaaccgcaa cgtcttttca ttgaagcacg cgctcgccag agaggcttga
     2161 agaacataac catcatcaca agtgacatga attcttttga gccttcggac acatttgatc
     2221 gaatcgtttc agttgaaatg tttgaacaca tgcgaaacta cgaaatcctg tttgatcggc
     2281 tgtcaaagtg gttgaatctg ggaggaaagc tctttgtgca catctttacg cacaaacaca
     2341 tttcctatct tttcgaacca cgcgattctt ctgattggat gtcacgctac ttcttcacag
     2401 gtggaatcat gccttccaat catctgctct tgtatttcgc acagggattc acaatctccg
     2461 atcactggat tgtaaatggg cagcactatc aaaagacgtc agaagcctgg cttaagaaca
     2521 tggacaacaa cctgcatcga attagaagac tttttgatga aacctacggc gccgaaagca
     2581 gaaagatgat cgaatactgg agaatcttct tcatggcagt ggctgaactc ttcggaacaa
     2641 aaaagggaga ggagtggatg gtgaatcact atctatttga aaaaactggc aaccgcggaa
     2701 gctgaattgg aatcaccgcg cgcgatgcag aatctcctgc agttcctctg tttgaagaac
     2761 aatgggattg cccttcatgc tgctcgccgc ggccgctttc acgcataact cagagaagtg
     2821 tgagtccgtg acgccaaagg aagagatgcc tgagattcca agatggtgcg caagatcaga
     2881 gacccatttc actccatcct ccgctgtgac actctggtcg ttggtcagga tctgagcaac
     2941 gaccgcataa cgttccaggg aaggcgaacc aggatcacgc ccgcgaagag ctcgaatgtt
     3001 cacttccatc acaggagcaa gtagagcggc gcacactgca ccatgaggag catcaaacgt
     3061 acctcccaga ggcgcggcaa atccgtgaac tgcaccaagc ctggaattcg caagagccat
     3121 accacccatc aaagaagcaa gcgacatgtc gcttctcgcg gccagatcgt tgccgttctt
     3181 aaatgcgggc aatagcgagc gagcccgcat caagccttct ctgcaaatcg catctgtcat
     3241 gggattgttc gcatgagaaa caaacgattc cagaatatga gagagtgcgt ccattccagt
     3301 attcgcagtt agcgccggtg gcatagtcaa cgtaagctcc gggtcaacgc atgcgatcaa
     3361 gggaaggagg agaggactgc gaagactgac tttcaacgaa tgctccggag aagttaagac
     3421 agcattgcga gtgacttcag aacctgttcc tgaagtggtt ggaatcgcga cgagtggagc
     3481 tgcacgatgc gcaatcgtct tccctttccc gatgatctcc aggtagtcca gaatgtcccc
     3541 tggatttgtc agaagaccgg ctacggctct ggctccatcc agcactccgc caccaccaag
     3601 gccaatcacc actggttcgc gcacattgtg taagtctcgc ataacagccc gtatttgatc
     3661 tatgcgaggt tctccggcaa tttcgcattc gacgaccggc atctcaagca agcctgcaag
     3721 cgcttgcgct ctctcgcggc tcttgccgtg cacaattacg gccgtcttgc ccaggaggcg
     3781 aatgtcagaa gctatttccc gaaaaactcc gggaccaaag acaattcgtg tagaagtcgc
     3841 gaattcaaaa cgcactttat ccccagccag agtcatctgg aaacgagttg gaatattttg
     3901 tactctgccg tggttcagcc atcatggatt ccacagtgtc acgccatttc ttatagtgct
     3961 cggtttgctt gtgatccgct gcgtccgatt ctttgcgata cacttctatc agtaaaaaac
     4021 gggaaggatc ttctctattt tgaagaacgt caaagcgagc aatccctggc tcctgcacgc
     4081 tcatcctcgc gttctccagg gaagcaatgc gaaagctttc gattgcattt tctttgacgt
     4141 gaacactcac ctggacaatg aacatggcaa cctctcagga aacaacgtga tccagcgagt
     4201 cgacacgcgc attcataata ggaatccata gaggcggaag aacagcgagc atgagcatgg
     4261 ccggataacc tgcgggcagc tgcggactat catcaaaatg acgaaggatc tggtacttcc
     4321 ggctggccca ggcatgatga tccgaatgcc gcggaagatt aaacagaaac aatctaccga
     4381 tgatgtgatt tgaattccac gaatgttgag gtcgaactct ctcgtacctg tctcccgttc
     4441 tctggcgcga aagtccgtag tgctctatgt aattcaccgt ttcgaggaga aggattccaa
     4501 tcaacgcgga cagaagaaat gcaagcagag gtagagtccc cagaaaaaga gcgatcccca
     4561 cgagaaagac ggcctgcaaa gtctgatcta cgagcattgt gttatgaata gatatgaacg
     4621 catgcccgcg atctctggtt ctcttcgctg atatcttcca cgcggatata taacccatga
     4681 caatggaacg aatccagaaa gtatagagcc attctccgcg tctggagctt gcaggatcaa
     4741 gttctgtggc aacgtttcga tgatgtccga ggttgtgttc tacgaagaag tgcgcgtaaa
     4801 gagaggtggc tagcgaaagg cgcgccattc tttgcgaaaa agaatccgca cgatgaccca
     4861 gctcatgccc tacattgatt ccgtaaacgc cgcatgcaag tccagttgaa atgatgagcc
     4921 cgctcaattg cagaaggttc tgacttctgg acgcctcaat caagagcatg ccgatgagac
     4981 cgcattgaaa gatgacggcc gcgtttaaca aatctgaaaa gatagtgctt tgacccagag
     5041 tgcgttcgtc ggtcggattg tttacgtcac gcgacagaaa cggcaatgtt tcgagaatcg
     5101 ggacaatgcc aaagaccagg accacaggcg cccagaccca tggtccctga agaaaaaagg
     5161 cgccatagca ggtacccatc aaaacaaacg ttgcaagata ccagatcgcg cccatttttc
     5221 taactccagt tttatattcc gcttaacggg tcactttcgt gtagtaacca ttgttcgtgg
     5281 cgactcccag tacgtggccg tctttctgat ccaggataaa agcatgctgt acgttgccat
     5341 acccgccgtc aaacacagct ctgcattcag gcacaacagg ataaggcggg gagattcctt
     5401 tttgaaacca gattcctccg ccctgcaaaa gcacgcttgc cacttcgcct gaggcaggct
     5461 ctctgtactc tcctgcaaaa cgcggcagct cagtttccgg agaaacagac tgcctgaaca
     5521 caaatagcgg gcgagtttct cccgcaaact ccgtggctag attacccagc cttgcctgaa
     5581 ttccaacaag aactcccatt gtatcttctt cgtacttagg aggatctccc agatagaaaa
     5641 gagcaacgtt gctgcgcggg aagatttgaa gccaggcagc cacaccgttt gcgcccccta
     5701 cgtgatacat ctgcacaata ccctgaccat ccgtacggac tcgccatccg attccgttgt
     5761 agtcctgaaa ctccgccggc ggcgcataga tctgattgcg aagcatggcg tccacgctct
     5821 gcgcacgcaa gaaccgcgcg tctttccgta agcctcgcct caaaaatacg gacgcgaatt
     5881 tggccagatc cgtcacgttc gtcatcacac cgtaagctcc agtggaacct ggaagcatgc
     5941 gcgaatcgtt cattcctgct gccgcgtata cttcctgatc aatgatgtcc gaaatttgtc
     6001 tgtgtgatat ctttttaaat gcttcgccta acagttgata gccgagattc gagtatcgga
     6061 attgcactcc tgcaggagcg gtctgtggag agattggaaa atcaatctct ggatgatgaa
     6121 tcatttcagg aggaacatag taacgaaggt ctggcattcc ggaagaatga gacagaagat
     6181 gccgaagcgt caccgcggga gaatgataca tctcttgttc aacgtgaagc ccggggaaca
     6241 cgtctgacag gcgttcatca aaatcaatga tgcccttttc ctgaaggcgc atggcagccg
     6301 tcgcagtgaa tacctttgta atggatccca cagaatagag acgagatggc acggaattga
     6361 tgtagcggct gtagaccagg tcatcaccac ggacgacacc tacagtcatg ctatagaaca
     6421 caccctttgc aatgtgatac tggatgtacg cctcgagatc ataggaaaga gacctgattt
     6481 tctcctcgtc cacatggaca ggacgaaccg tgcgcaccgg ctcctcgctg gtgtagcaat
     6541 gagtgaatag aacgagcaac agtagtccga gccgggccat agacgtccag cctgactggc
     6601 tccgttaaaa agagagtcaa accaaaatgg cgcggccgag agccagtttt tcctgggtca
     6661 gatcctccgt tttccatgcc tttgaacccg ggcagggctc cgccccccga ggggcaaaaa
     6721 atatttgacg atatatccat cgtggatata tccatcctgg acatgccttc caccgaaaaa
     6781 ctctctccag ctgctctgga aatcctgttg atccttgcgg gacaatccat gcacgggtat
     6841 gcaatcatga agacttctcg cggagaagga ggcgttcgcc tgggtccggg tacactctac
     6901 cgctggctgg actctctttc tcaaaccggt tacatccgca tcaagaaaga agacggtcgc
     6961 agaaatctat atgaaatcac accttcggga aggaaggtct tacgcgatga gctttctcat
     7021 gcccagaatc tactggcgcg agcgaaggta gcgggcattc gcacccaggg gagtggcgga
     7081 tagaaaatga agccgagaaa gcatcaaggt cgcataacgc tacgcagaca gctgtttttg
     7141 catgtattct atccagagga atttctcaaa acacatctgg aatccatgtg cgaagatctt
     7201 tctgatctag agtcagaagg agcgctgcag gcacgtgacc tcatagatct ggcactgaac
     7261 gggctgaaaa tcagaatcaa gcatattgcg cgccggatgc gcccctatca tcacgctctt
     7321 ttgaccgcaa cggttgcctt cccgctattt gcggcgttct ctgccatttc atttctcttt
     7381 cttccgatca atctcctccc ctggattctg gactttccct ttcaggattc actcctcatg
     7441 cgcgcggcga tcgttctttc cctggtatcc tctatcctct tgatcgcgag cgaacgttcg
     7501 ccaaaaaact ttctgtgttt gtgtatcaac gcccctgtaa ctctattctg gaccgttctc
     7561 ttattttgaa tcgcggagaa aattcatgaa aatcgctctc atactactct cagtcgcttt
     7621 tgttttcatt gcgggcttcc ttgcctattc cggagctctg tccacggtag tggtgaagga
     7681 agaaaaagtc gggccgtttc acattgtcta tcgagaagtc aaaggcagct acaggcaaac
     7741 agggaaagtt tccatggaaa tccagagctt gctcaaagaa aacggaatag acaatgatcg
     7801 attcttcgca gtttacctgg acgatccgag atccgtgcca gaggaaaacc ttcgcagcct
     7861 ggtggggcac attcttcccc cggggtccag ggaagcaaaa cttccagcgg aatataagag
     7921 cgccatcctc cctgaaagac ttgcactcgt ttcagtcttt ccactgaaaa cgcaggcttc
     7981 catttttctc ggaatgttca aagtatatcc ggcaattttc gcgcaggcag ccaaatctgg
     8041 ttatcctgac aaaaagcctt ctcttgagat ctatgag
//
LOCUS       DAORFF010000105         8014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4411_length_8014_cov_3.484986, whole genome shotgun sequence.
ACCESSION   DAORFF010000105 DAORFF010000000
VERSION     DAORFF010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4411_length_8014_cov_3.484986"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PKK76_05950"
     CDS             <1..184
                     /locus_tag="PKK76_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68046.1"
                     /translation="TGTPKADPRVFPDTKPDIYWTRESYNNSPITNWVVDFGAGGSFG
                     YGTENQAYVRCVANLP"
     gene            249..1289
                     /locus_tag="PKK76_05955"
     CDS             249..1289
                     /locus_tag="PKK76_05955"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNL68047.1"
                     /translation="MHGALPGEKVRVRLTKRNRNHSFAVLESVIEPSPSRIESDCSIF
                     PECGGCSFRNISYEEELSVKTRLVREFRPLGEACGDFEVFSAAPDAYRTHVRIQFDGK
                     QAGFFSLHSNRIVPLPSTGCANLPKELNSAIFGFIKKETRARTFAPRDLRFRISDKVY
                     GPSHHGTVPIAIGSLTLDTPADGFVQANSMLIETWLKFIASLIGPGLPPALELYCGSG
                     IIGAYVRENLGSYRAFDFNSSSIECARKNFSNSGLEGQFDVMDLNEEMPEPASLCIVN
                     PARPGLSACVREGLKASSVRSIIYSSCNPATLNRDLVELLAAGFFVRKSAMFDFFPRT
                     PHVEMVCLLERK"
     gene            1286..1780
                     /locus_tag="PKK76_05960"
     CDS             1286..1780
                     /locus_tag="PKK76_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68048.1"
                     /translation="MRWFVVLCLFAPFCTPSALIEKKYATYNFTEEGFLSPRLIQTIG
                     KSRYPAGSYTIDSSRMRCLENSLEMARERMLRIFVFTRFGLKARKTKDQRTFDDDYPH
                     ALSARTLRRAEADFSVLLDRATIALQDARSQESCIVVYRLAGDSLPEEIKAVQTTFVP
                     DLAP"
     gene            1777..2160
                     /locus_tag="PKK76_05965"
     CDS             1777..2160
                     /locus_tag="PKK76_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68049.1"
                     /translation="MRKLAICLSLFVLACSSREVQVPEDTDLTEGMISSSTYQVVLSA
                     FGADEQEARKRGEEDARRKTYMLILREPFFRGTLSEEGKRQIRTLIETRGRVVSVQKE
                     AEGAYRLIFQVTQPGGLRNTFRSIR"
     gene            2200..3735
                     /gene="gltX"
                     /locus_tag="PKK76_05970"
     CDS             2200..3735
                     /gene="gltX"
                     /locus_tag="PKK76_05970"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNL68050.1"
                     /translation="MSSPIRTRFAPSPSGFLHVGGARTALFNYLYAKARGGQYVLRVE
                     DTDQARSTRESERMILESLNWLGIVPDEGPDEGGPYGPYRQSERLDIYKKYTTKLLEE
                     GLAYPCFCTEEELEQMQESSRKLGIPNIYDGRYANLPKEEAEARIKAGDPYSIRLRVG
                     AEEVVVNDLVQGKVKFDSRLIGDFIIVKSDGFPTYNYAVVIDDNEMKISHVIRGVGHL
                     SNTPRQILIHRALGLDLPQYAHISEIVGTDKKKLSKRRGATSILFFRDLGYLPEAFNN
                     YMALLGWYPEDGAEFMSSADLKRKFDVARCSKSPSMFDFFLTDKGGDAEDAKPEGMTL
                     DELKKAINKKTKLNWLNNKYIRAADLDEIWPEAARFLSRDKELADAVKKDPDKMRKIF
                     SEVRIYLDTFDDAAPFYRELLKQKIALEPGEAAEVLALPEAKPVIEAFKQIVKETRPT
                     TPEGFSEAMKQAGVQTGAKGRALFMTIRVAATGSMTGLELPNLFAILGPDQVLSRISQ
                     VAG"
     gene            3754..5310
                     /locus_tag="PKK76_05975"
     CDS             3754..5310
                     /locus_tag="PKK76_05975"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNL68051.1"
                     /translation="MQDWIPSLQDPVSYDHSVSKIELRETHISWVFLTGRFAYKIKKP
                     VDFGFLNFTTLEKRKFYCEEEVRLNRRLAPDIYLSVVPITNKDNHPFISRVALPGAEI
                     LDYAVCMAQFEPDLTLDRIESNEGLSGEQVDIIAETVADFHSRVERAALESAFGWPDQ
                     VIGPPRDNFKDTHVQGFETRLGQIAEWTEKEWARLGPVFEKRKRNGFIRECHGDMHLG
                     NIALVNDKPVIFDCIEFNEPFRWIDVQSDAAFLVMDLVERGHKDHAFRFLNRYLECTG
                     DYEGLLVLRYYLVYRAMVRAKVASLRMHQEGANVPELVLSFEHYVQMAESFANSRNPA
                     VILTCGLSGSGKTRASREFLARGMIRVRSDVERKRIFALAPEEKSGSALDQGIYTQDA
                     GKKTYDRLLYVMRVILESGYPAIVDATFLEESMRAPFFAFALELKIPLVVAHCHAEPD
                     VLRERVIRRMAKGKDASEADLNVLESQLKKGYGFSDAEKKSLVEIDTGNEEDRARAVQ
                     EVLRRAGAFE"
     gene            complement(5332..7776)
                     /locus_tag="PKK76_05980"
     CDS             complement(5332..7776)
                     /locus_tag="PKK76_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNL68052.1"
                     /translation="MRIRNLCFNALALVWSAGLFAQEKFDPASVRSRDCGPPTYSCGF
                     TAPDPERARSIPVVPAVRMRGLPSSVDLSSQMPPVSNQGQQNSCVAWATTYATRSYFE
                     QKARQWGYDAPVKGGRGEHIFSPAYVYNQINGGRDQGSVMENAFDLLVRQGAAPWSVM
                     PYTDKDYTTQPTAAQRSAASKYKLASYRRIPPGDIETIKAELAAGRPVVFGMGIDDNF
                     YQLKNSVYDQKGGKTYGGHAMALVGYDDGKSSPRGHRGAFKIINSWGTGWGDKGYGWI
                     SYRQWTSEQPWALVGYPVDTPTQTQTTTTTTTNPTPAPTPPEPVSEVTVPPPAQVNAT
                     KGTYTDKIVVSWAAVENAVVYAVARAAPDKEFEVLGYSQEPTYEDKAVQQSVAYRYIV
                     VAAVSNENYSDPEKSPVAEGYTAKATPVVRPPEKVTGVSATVTRQGALPVVSLDWTAA
                     ANASSYQVARYNAAAGQWQTIATVTAVEFEDRNPPANTKAYYAVRGVSNTAGKWSDTA
                     EVQLAGATTPPSTPSGLVVSDGVYKEKIEVTWDAVPGAARYFLFRYDYGAGTWAPPIS
                     SETNSYMDQDRAVASGKNFAYTVVAQNDAGSSGFAPPVIGKTNPNAARGVTLLAPKNV
                     KGTITGTTVNLSWTAVPGAKEYSVFKAKKGGKPDFVASVSTTSYSGAFKEKPGELYFY
                     TVRSKSEYGGESADSKGVALFVNQTRSIPRERALPDQGIDRYTGTWNGRYMEKGAKPH
                     DVVLKFASKGLEVHGTILMDNNPVVFKGDYATGATYLMTDQVDMHLLDFGLLELTFKT
                     PGKGEVTAVVEKDAEK"
BASE COUNT         1911 a         1960 c         2098 g         2045 t
ORIGIN      
        1 taccgggacg ccgaaggcgg atccaagagt tttcccggac accaagccgg acatctactg
       61 gaccagagag tcttacaaca acagccccat cacaaactgg gtcgttgatt ttggcgccgg
      121 tggatctttc ggatacggca cagaaaatca agcctacgtt cgctgcgttg ccaaccttcc
      181 ttgaacttcg cccgctgaag tgggtacaga acggatttac gctggctccc ggggagcatc
      241 ctgtatttgt gcacggagct ctcccaggag agaaagtgcg cgttcgcctg acaaagcgta
      301 atagaaatca ttcgttcgca gtcctggaaa gcgtaatcga accttctcct tcacgcattg
      361 aatctgattg cagcattttt cctgaatgcg gtggctgctc ttttagaaac atctcatacg
      421 aagaagaact atccgtaaag acaaggcttg ttcgtgaatt tcgcccactg ggagaagcgt
      481 gtggcgactt cgaagtcttt tccgccgctc cagacgcata tcgcacccat gttcgcattc
      541 agttcgacgg caaacaggct ggtttctttt ctcttcattc aaatcgaatt gtgcctcttc
      601 cttctactgg ttgcgcgaat cttcctaaag aattgaatag tgcgattttt ggattcatca
      661 agaaagaaac gcgcgcgcga acatttgctc ccagagatct tcgctttaga atatcagaca
      721 aggtctatgg cccatcgcac cacggtacag tgccgattgc gattggatca ctgactctcg
      781 atactccggc agatggattt gttcaggcca attccatgct cattgaaacc tggctgaaat
      841 tcattgccag tctgattggt cctggtctcc cgcctgctct cgagctctat tgtggcagcg
      901 gaatcattgg ggcctacgtt cgagagaacc tcggaagtta cagagcgttt gattttaatt
      961 cctcaagcat tgaatgtgcg cgaaagaatt tttctaactc aggactggaa ggtcaatttg
     1021 atgtgatgga tctgaatgaa gaaatgccgg agcctgcatc tctctgcatt gtaaatccag
     1081 cgaggccagg tctcagcgcc tgtgttcgcg aaggcctcaa agccagcagt gtgcgaagta
     1141 tcatctattc ttcgtgcaat ccagccactc taaacaggga tctagtggaa ctacttgcgg
     1201 ctggtttttt tgtccgaaaa agcgcaatgt ttgacttttt tccaagaacc ccccacgtgg
     1261 aaatggtttg tctcctggaa agaaaatgag gtggttcgtc gttctgtgtt tgtttgcgcc
     1321 cttctgcacg ccttctgcac tcattgaaaa gaaatacgcg acgtataatt ttacagaaga
     1381 aggctttctt tcgcctcgtc tcatccagac cattgggaag tccagatacc cggccggatc
     1441 ctatacaatc gattcgtctc gcatgcgctg cctcgaaaat tccctggaaa tggcgcggga
     1501 gagaatgctt cggatttttg tttttactcg ttttggactg aaggcccgca agacaaagga
     1561 ccagcgcacc tttgacgatg actatccgca cgcactttct gcccgcacat tgaggcgagc
     1621 ggaggcggat ttctctgttt tattggacag ggcaaccatt gctttacagg acgctcgatc
     1681 gcaggaaagt tgtattgtcg tctatcgtct ggccggagat tcgttgcctg aagaaatcaa
     1741 ggcagtgcaa acgactttcg tgcccgatct ggcaccatga gaaaactcgc tatttgctta
     1801 tcattgttcg tacttgcatg ttcctcccgc gaggtgcagg taccggaaga tacagacctg
     1861 acggagggga tgatctccag ctccacctac caggtggttc tatctgcatt tggtgcggac
     1921 gaacaggaag cccgcaagcg cggggaagaa gatgcgcgga gaaagacata catgttgatt
     1981 ctgcgagagc cattctttcg cggcactctg agtgaagaag gaaagaggca aattcgcaca
     2041 ctcattgaaa cacgcggccg cgttgtgagt gtgcagaagg aagcggaagg cgcctatcgt
     2101 ttgatctttc aggtaacgca gccaggcggc cttcgcaaca cgttccgttc cattcgatag
     2161 aacatagttt tgattcaatg atgatttgag aggaatacaa tgagttcacc cattcgcacg
     2221 cgatttgccc cgtctccttc tggatttctc catgtcgggg gagcgcgcac tgctttattc
     2281 aactatctat acgccaaggc acgcggaggc cagtacgtat tgcgtgtgga agatacggat
     2341 caggcaagat cgacgcgcga atcagagaga atgattcttg aatctcttaa ctggctgggg
     2401 attgttccag acgaaggtcc ggatgaaggg ggtccctatg gaccttacag gcaatctgag
     2461 cgcctggaca tatacaagaa gtacacaaca aaactgctcg aagaagggct cgcttatcct
     2521 tgcttttgca cggaagaaga actcgagcaa atgcaggaaa gctcgcgcaa gcttggaata
     2581 ccaaacatat atgatggacg ttatgcgaac ttgccaaagg aagaggcaga ggctcgcatc
     2641 aaggccggtg atccgtattc gatccgtctt cgcgtcggtg cggaagaggt agtggtcaat
     2701 gatctggttc aaggaaaagt aaagttcgat tccaggttga taggagactt catcattgtg
     2761 aagtcagacg gatttccaac ctacaattac gccgttgtca ttgacgacaa tgaaatgaag
     2821 atttcgcatg tgattcgagg tgtcggacat ctttccaaca cgccgcgaca gattttgatt
     2881 catcgcgcac tggggcttga tcttccgcaa tacgcgcata tttctgaaat tgttgggaca
     2941 gacaagaaga agctttcaaa aagaagaggg gctacctcga ttcttttctt cagagacctg
     3001 gggtatttgc cggaagcatt caacaactac atggcactcc tgggatggta tcccgaggac
     3061 ggtgcggaat tcatgtcttc ggcggatttg aagcgcaagt tcgatgtggc gcgttgctcc
     3121 aagagtcctt ccatgttcga tttcttttta acggacaaag gaggggacgc agaggatgcg
     3181 aagccggaag gaatgactct ggacgagctt aaaaaggcaa tcaacaagaa gacaaagcta
     3241 aactggctca acaacaaata cattcgtgca gcggacctgg acgagatctg gcctgaggca
     3301 gcgcgctttc tttcgagaga caaagagcta gcagatgcag tgaagaaaga tccggataaa
     3361 atgcgtaaga tcttttcaga agtgagaatt tatctggaca ccttcgatga tgcagcgcca
     3421 ttctatcgtg agctgctcaa gcaaaagatt gcactggagc caggagaagc ggctgaagtg
     3481 ctcgctttac ctgaggcaaa gcctgtgata gaagccttca agcaaattgt aaaagaaact
     3541 cgccccacca ctccggaagg tttttccgaa gcaatgaaac aggcgggagt tcaaacaggt
     3601 gcgaaaggcc gagcgttgtt tatgacaatt cgcgtggctg caactggcag catgactggt
     3661 ctggaacttc ccaacttgtt tgcgattctg ggacccgacc aggttctttc gcgcatctct
     3721 caggtggcgg gctgagtaaa gagggaatga accatgcaag attggattcc ttcccttcaa
     3781 gatccagttt cctacgatca ctctgtttca aaaattgaac tgcgtgagac ccacatttcc
     3841 tgggtgtttc tcacaggccg cttcgcctac aaaatcaaga aaccggtgga tttcggattt
     3901 ctgaatttca ccactctcga aaagagaaag ttctattgcg aagaagaggt tcggctcaac
     3961 cgcagactgg ctccggacat ttatctatct gtagttccca taaccaataa agacaatcat
     4021 cctttcattt cgcgcgtagc tctgcctggc gcggagattc tggactacgc ggtatgcatg
     4081 gcccagtttg agccagacct gactctcgat cgcattgaat caaacgaagg actgagcggg
     4141 gagcaggtgg acatcattgc ggaaacggtg gctgatttcc acagccgcgt tgagcgcgct
     4201 gcactcgaaa gtgcgtttgg ttggcctgat caggtgattg gtcctcctcg cgacaatttc
     4261 aaagacacac acgttcaggg atttgaaacc aggctcggtc aaattgcaga atggacggaa
     4321 aaagaatggg cgcgccttgg ccctgtgttt gaaaaacgca agcgaaatgg attcatccga
     4381 gaatgtcatg gagacatgca cctgggcaat atagctctcg tgaatgataa acccgtcatc
     4441 tttgactgca ttgaattcaa tgagcctttt agatggatcg atgttcaaag cgatgccgct
     4501 ttccttgtga tggacctggt ggagcgtggg cacaaagatc atgcctttcg tttcttgaat
     4561 cgttatctgg aatgcacggg cgactacgaa ggtcttcttg tgttgcgtta ctacctggtg
     4621 tatcgcgcta tggttcgcgc gaaagtagcc agtcttcgca tgcatcagga aggcgcgaac
     4681 gttccagagc tcgtgctgag ctttgaacac tatgtgcaga tggcagaatc atttgcaaac
     4741 tctagaaatc cagcagtgat ccttacatgc ggcctatccg gttccggcaa gaccagagcc
     4801 agtcgtgagt ttctcgcacg gggaatgatt cgagttagat cggatgtgga gaggaagagg
     4861 atttttgcgc tggctcccga agaaaaaagc ggatctgctc tggatcaggg gatctatact
     4921 caggatgcag gcaaaaagac ttacgatcgt ctgctctatg ttatgcgagt gattctcgaa
     4981 tccggatatc ctgcaattgt agatgcgacc ttcctcgaag agagtatgcg cgctcctttc
     5041 tttgcgtttg ctcttgagtt gaagattccg ctcgtggtag cgcactgcca cgcagagccc
     5101 gacgttcttc gcgaaagggt tatcaggcgg atggcaaagg gaaaagatgc aagcgaagcg
     5161 gatctcaatg tcctcgagtc tcagctcaaa aaaggctacg gattcagtga tgcggaaaag
     5221 aaatctctgg tagaaataga tactggaaac gaagaggatc gcgcccgggc cgtgcaagaa
     5281 gtgctacggc gcgcgggcgc ttttgagtga gaaaacggag tctggctcgt tttatttttc
     5341 cgcgtctttc tccacgactg cggtgacttc ccctttgccg ggagtcttaa acgtaagctc
     5401 aagcagtcca aaatcaagca ggtgcatgtc tacctgatct gtcatgaggt atgtcgcgcc
     5461 tgtggcatag tcgcctttga acacaacagg gttgttatcc atcagaattg tgccgtgaac
     5521 ctcaagacct ttgcttgcaa atttcaaaac aacgtcatgc ggctttgcac ccttctccat
     5581 gtaacgtccg ttccaggttc ctgtgtatcg gtcgatgccc tgatcgggca gggcacgttc
     5641 gcgcggaatg gatctggttt gattcacaaa gagtgccact cctttagaat cagcagattc
     5701 tccgccatat tctgacttgg atcgcacagt gtagaagtag agttcgcctg gcttttcttt
     5761 gaatgctccc gaatagctgg tcgtagagac gctggctaca aagtctggct tgcctccctt
     5821 cttcgctttg aatactgaat actctttggc acctggaacc gcagtccagg aaagattcac
     5881 agtcgttccg gtgattgtgc ccttcacatt cttcggagca agcaaggtaa ctccgcgcgc
     5941 agcgttcgga tttgttttgc cgatcacagg tggagcaaat ccgcttgagc ctgcgtcgtt
     6001 ttgtgcgacc actgtgtagg cgaagttctt accgctggcg actgctctgt cctgatccat
     6061 gtacgaattt gtttcagaag aaatgggagg agcccaggtg cctgcgccat agtcgtatcg
     6121 aaacaggaag taacgagcgg cacccggaac tgcatcccag gtgacttcga ttttttcttt
     6181 gtacactcca tcggatacta cgagacctga aggagtggaa ggaggagtgg tagctccggc
     6241 cagttgcact tccgccgtgt cgctccattt tcctgctgta ttcgagactc cgcgaactgc
     6301 atagtatgct tttgtattgg ccggaggatt tctatcttca aactcaacgg cagtgacagt
     6361 cgcaatggtt tgccattgtc ctgcggcggc attgtagcga gccacttgat acgaagaagc
     6421 gttcgcggcc gcagtccagt ccaggctcac cacaggcaaa gcaccctgtc gcgtgacggt
     6481 cgcggaaaca cccgtaactt tttcgggagg acgaactaca ggagttgctt tggctgtgta
     6541 accttctgct acaggcgact tctctggatc cgaatagttt tcattggata cggcagctac
     6601 cacaatgtaa cgataggcga cactctgctg cactgctttg tcttcgtagg ttggttcttg
     6661 cgaatatccc aggacttcaa attctttgtc cggtgcagca cgtgccactg catacacgac
     6721 tgcattttca accgcagccc aggacaccac gattttgtct gtgtacgttc ctttggttgc
     6781 attcacttga gcgggcggag gcactgtgac ctcagatact ggctctggag gagttggtgc
     6841 tggagttgga ttggttgtag tagtagttgt agtctgcgtt tgtgtcgggg tgtctacggg
     6901 gtatcctact agagcccagg gttgttctga agtccattgc ctgtaggata tccatccgta
     6961 tcctttgtcg ccccagcctg ttccccagga gttgatgatt ttgaatgcac ctctgtgccc
     7021 gcgcggagaa gatttaccat cgtcatagcc aacgagcgcc atcgcatgcc cgccgtatgt
     7081 ttttcctccc ttctgatcgt agactgagtt tttcagctga tagaagttgt cgtcgattcc
     7141 cattccaaat acaacaggac gacccgcggc cagttctgct ttgatcgttt cgatgtctcc
     7201 gggtggaatt cttctatagc tggcgagctt gtatttcgat gccgcagaac gttgcgcggc
     7261 agttggctgt gtggtataat ctttgtccgt gtaaggcatg acgctccatg gagctgcacc
     7321 ctggcgaacc agaagatcaa aagcattttc cattacgctc ccttgatctc ttccgccgtt
     7381 gatttgattg taaacatacg ctggtgaaaa gatgtgttcc cctcttccgc cttttacagg
     7441 agcgtcgtat ccccattgtc ttgctttttg ttcgaagtag gaacgcgtcg catacgtcgt
     7501 ggcccaggca acgcagctat tctgctggcc ctgattggaa acgggaggca tttgagagct
     7561 taagtccaca gaagaaggga ggccgcgcat gcgaacggct ggcaccacag ggatggacct
     7621 tgctctttct ggatctggcg cggtgaatcc acaggaatac gtgggtggac cgcaatcgcg
     7681 cgagcgaacg gacgctggat caaatttttc ctgggcaaac agccccgcag accagacgag
     7741 cgccagagca ttgaagcaaa gatttcgaat tctcatgcaa tgtcctcggt cctgtttcgt
     7801 ggcatttccg cgcacacagg cacctcttga tgtagactct ctacacccgt ctggagttcg
     7861 tcacaagtaa aaattgtttt ccggcccggg cgtcaggccg ggatttttgt tagaatgatt
     7921 ccaccgacca caagagcgat tctgacaccg tattccaccg caatgagcgc cgccgtttct
     7981 gcgatttcct tcttgattcc atgctctacg attc
//
LOCUS       DAORFF010000106         7970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4462_length_7970_cov_3.203917, whole genome shotgun sequence.
ACCESSION   DAORFF010000106 DAORFF010000000
VERSION     DAORFF010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7970
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4462_length_7970_cov_3.203917"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="PKK76_05985"
     CDS             complement(<1..1121)
                     /locus_tag="PKK76_05985"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HNL68053.1"
                     /translation="MTQATAFLAAILALAGGWLIYSQIRFLRRWFIPPALLGGLLLFC
                     AGPEVLSIVGKPERLFIRSWPPLLLGYLFAAIVLGFESGKSSRGLVGQVVQQNLFVWF
                     LSFGQLLGAYCIYLLFFRADVSPLIAHVVEIGWAGGPGSAAAMSAVMRRLGQSAIGDL
                     GLFSATLGQVWGALSGIWIVNHLNRKAQHVIGTEASSSHESARPLSSVEANGIADAEV
                     RRPRFPGPKGLLRIFLLFLLPLSAVFIAMAAKEGLGIVAIYTPRALFLGDIPLFSLAL
                     LCAFPVRWIAIRAGILTAENCETIQNINGQVLDWIILAALAALEPAAFIALLVPFSAM
                     MAFGAFFCFVLMYFFAPRMLPTNTWRELGLINYGMATGTT"
     gene            complement(1118..3241)
                     /locus_tag="PKK76_05990"
     CDS             complement(1118..3241)
                     /locus_tag="PKK76_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68054.1"
                     /translation="MEWLLLLLAGGVAAGVGLWAVRPSPGEQGSSEGPSSSPGLPGVK
                     DDRGTAVPLGGKDTTDQIRDLLAKAPDKIQAKGVSRKQEPSRSSKVPLRVTYNTDQLI
                     ARSSRFAHHRRALQNAEILVSKQKATEALEVFERVANRVQDADIQSRIQTNIDDIRRW
                     LSGFESEDDQLKFPEIVIPLTLQNLALENLTEGIKNLSDSIVQKIAESVAAVSAISVQ
                     HTSPSGGYATSSPGSAPASPSASAPASSPASAPSAYAATPGAPAQTAMPGQAPGAAIP
                     TMGAAYAVPATADMPAQAILPVIPRIVPPSGEWVREGYGKPGQETGVAQRNLPPGISL
                     NERGELVTDGWTDSDFDRAWEKFKHLPAHDRRSGVERRKNIDRRMSNDPKRKDRRSGE
                     DRRKQDLFKEREEFLRKLEEHKKRKQEYADWLANEELPVLPPVEMPVEEEKKKKEATI
                     EIEHATIHIGEFPKKEEEKDEEPEEEPEPEPEPEPEPEEEPEPEPEEEEEEEPKHIVF
                     ETPEPDLQPVRMPAVGEFAGSHLEAARDLSSQPLLSLGMPSGMKVDDQPQLPAEAEAA
                     TASAGETAATEEETPEIPGLDEPEEAKEEKPQVQEIRGVLELKPPEEDDAPFLTLTYD
                     FTKIPDSFKLSRDYHTMEYAYYKYKPMLVKAQEFTRRKMLKNALNYYRVIKSQNIPPE
                     FKRMINRNIRDITEYLEKFLMSRAG"
     gene            3309..4622
                     /locus_tag="PKK76_05995"
     CDS             3309..4622
                     /locus_tag="PKK76_05995"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68055.1"
                     /translation="MALPIQKINSRLEGEFVFRGRPYSVPFTLPGDLVQFKLSRKTGR
                     LGMEVLHIEKAHNTDFALADPFCAVYGRCGGCRGQHIPYPKQIELKTTDLVRAMEGLG
                     KKPEILVAPRLQGHRNRMDFVVDAGVTGFREAREFARFVDVDLCPAERDQSNRVLEVF
                     RSAIERHKDIAFIRESGEGIVKYATIRSGSTSLLSITIEASRSENQRYLAFLQDLIQT
                     LPPQCSLVETHTVYPSDVSNPSGGKVHVGDGTYTDRLGGLDFRVAADSFFQPNPEAFD
                     LLLDHVMAWFGPGDVATERLVDLYCGTAVLSAIFTNRFPGDFASVYGADFTESAIAHA
                     PGNLKHFSDPVRFEALDLAQKMVPLLPGDFVILDPPRAGLAPQVLRSVLDGLPERVLY
                     ISCNPQTQLRDLRALTSTYRIDSAVISDCYPHTQHLEQAVFLRKL"
     gene            4619..4945
                     /locus_tag="PKK76_06000"
     CDS             4619..4945
                     /locus_tag="PKK76_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68056.1"
                     /translation="MSDSVFSGLWRRANSGAENWVNVLEIKQDNDHITGKLRLYEKQP
                     GGEEEILFESDVEGRVGAENAKIYLRTRPLHDAISLYIVERDDDRLRLLPMGHDNDLA
                     GAWVAQ"
     gene            complement(4939..5742)
                     /locus_tag="PKK76_06005"
     CDS             complement(4939..5742)
                     /locus_tag="PKK76_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNL68057.1"
                     /translation="MANLKEMVQSSEGGSLLKKGAIIGGIVILALILKPFTLISAGHR
                     GVVTNLGAVSDRVLGEGFNFKVPIYQTVVEMNVQVQKAKATAQAASRDLQTVETTIAV
                     NYHIDPAHVNHLYQNVGADYEATVIDPAILETVKAVTANYTAEQLITNRQNVSLEIQK
                     NLADRLKPFFLFVDAISVENFNFSEKFNEAIESKQQAEQMALKAQRDLERIRTEAEQQ
                     ITTARAQAEAYRLQTQTLTPLMRDMEWIKKWDGKLPNYYGSANPLLSIH"
     gene            5902..>7970
                     /locus_tag="PKK76_06010"
     CDS             5902..>7970
                     /locus_tag="PKK76_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL68058.1"
                     /translation="MKLRLPTEKTYRFSILQALLPIVIISIVGISLNFIIGSTRAVVE
                     LSDHLVEEVSQRMIERTLAHLEGGAMHNRVTANLVSDLDLMNNHETVWRYLWAPMIET
                     PQVQSLFVADRRGNYVQVRREPFLATRMIDAARGIETTVTRDESYREKETKTNPTTWD
                     PRTRPWYKNTTREKKMAWTDVFVFTTAKTPGLSASYPVLDAQGEIVGVIAANIPLHNL
                     SDFVAEQKVMQHGIVLIINDKGEVVAYPDKKVNLVVDSDAKEPDPKEHKEKEMRLLRI
                     DELPNQAVVDAYRRSESEKSTRVTSATNGEKYITTFKPFPAPFEKLKIVIVIPEADIL
                     ASIHRMELIAILVSVVILILGIIVVYQVSIRISDPIIRLAHEMDHIGDFSLDDVKGVQ
                     SKIHEIRIMNSSLLTAVEGLKAFRRYLPASFVRQLITSGKKAELGGIKTEITLFFSDV
                     EGFTRISESMPAEDLMLHLSAYFNELSRIILEEGGTIDKYIGDAIMVFWGQPVEIADA
                     PIRACRAALRCVERLTELNEKWSAEGKPTMRTRFAIHTGESIVGNVGTEERMNYSAVG
                     DSVNLTSRLESANKIYGTTIIISQSTFERVQHLFRCRLLDVVAVRGKTRGVRIYELLG
                     ESTVSPTEAQNRFVAGYEQAFSVYLSRNWQEAVQAFSKLNVEFPADKSVQVMLRRCDA
                     FAKDAPGL"
BASE COUNT         1827 a         1988 c         2107 g         2048 t
ORIGIN      
        1 gtggtcccgg tggccattcc gtagttgatc agtccaagct ctctccacgt atttgtgggc
       61 agcattcgcg gcgcgaagaa atacatcaga acaaagcaga agaaagcacc gaatgccatc
      121 atcgctgaga atggaacaag aagtgcaatg aaggctgcgg gttcaagtgc tgcgagagcg
      181 gccaggatga tccagtccag gacctggccg tttatgttct ggatcgtttc gcagttttca
      241 gcagtcagga ttccagcgcg gatggcgatc cacctgacgg ggaatgcgca cagtagcgca
      301 agagaaaaga ggggaatgtc tccaaggaag agtgcgcgcg gagtgtaaat ggcgacgatt
      361 cccagccctt cttttgcggc catcgcaatg aacacagcgg aaagcggaag aaggaacaat
      421 aggaagatgc gcagtaggcc tttcggtcct gggaagcgtg gacggcgcac ttccgcgtcc
      481 gcgattccat ttgcttctac ggacgacaga ggtcgcgctg attcgtgcga agagcttgct
      541 tctgttccaa ttacgtgctg agcttttctg tttagatgat ttacaatcca gattccgctg
      601 agtgctcccc agacctgtcc aagagtagca gagaaaagtc ccaggtcccc aatcgcagac
      661 tggccgagtc gccgcataac ggcagacatg gccgccgcag atcctggtcc gccggcccaa
      721 ccaatttcga ctacgtgagc aatcaaaggg gaaacgtccg cgcgaaagaa gagcaggtag
      781 atgcaatatg cgccaaggag ctgaccaaaa gacaaaaacc acacgaacag attctgctga
      841 acaacctgac ccaccagtcc tctggatgat ttgccggatt caaatccaag aacgatggca
      901 gcgaataagt atccaagaag aagcggaggc caggagcgaa tgaaaagtct ctctggtttt
      961 cctacaatcg aaagtacttc tggtcctgcg cagaaaagga ggagcccacc caggagtgcg
     1021 ggcgggatga accatcttct gagaaaacgg atttgcgaat agatgagcca cccgccggca
     1081 agtgccagaa tggctgctag aaacgccgtg gcctgcgtca tcctgcgcgg ctcatcagga
     1141 acttttccag gtactcggtg atatctctga tattgcgatt gatcattcgt ttgaactcag
     1201 gcggtatgtt ttgcgatttg atcacgcgat agtagttcag agcattcttc agcatctttc
     1261 ttcgcgtaaa ttcctgagct ttgaccagca ttggtttgta cttatagtat gcatattcca
     1321 ttgtatgata gtcgcggctc agcttgaagc tgtccgggat tttggtgaaa tcatatgtga
     1381 gagtgaggaa tggcgcatcg tcttcttccg gtggtttgag ttcgagaaca cctcgaatct
     1441 cctgcacctg tggcttttcc tcttttgctt cttccggttc gtcgagcccc ggaatttcag
     1501 gagtttcttc ttctgtcgct gctgtttcgc ctgcgcttgc tgtggctgct tctgcttccg
     1561 caggcaactg aggctgatcg tccaccttca tgccagaagg cattcccaga ctcaagagtg
     1621 gctggctgga taaatcgcgc gctgcttcga ggtggctgcc tgcaaattcg cccacggctg
     1681 gcattcgcac cggctggaga tccggctccg gcgtctcaaa gacgatgtgc tttggttcct
     1741 cttcctcttc ttcttcgggc tctggctctg gctcctcttc tggttcaggc tccggttccg
     1801 gctcaggctc aggttcttct tctggctctt cgtctttttc ctcttccttc ttcggaaatt
     1861 cgccaatgtg aatggtcgcg tgctcaatct cgatggttgc ttctttcttc ttcttctctt
     1921 cttctaccgg catttccacc ggcgggagaa caggaagttc ctcattggcc agccagtccg
     1981 cgtattcctg tttccgcttc ttatgttctt ccagttttct gaggaattct tcgcgttcct
     2041 taaaaagatc ctgctttctt ctgtcctcgc cggaacgtct gtcttttctt tttggatcgt
     2101 tcgacattcg ccgatcgata ttctttctgc gctctactcc actgcgtcga tcgtgtgcgg
     2161 gcaggtgttt gaacttttcc cacgcgcgat caaaatcaga atcggtccag ccgtctgtta
     2221 cgagttcccc gcgttcgttc agtgaaattc caggaggaag atttctctgt gctactcctg
     2281 tttcctgtcc tggttttccg tatccttctc tgacccattc tcctgaagga ggtacaatgc
     2341 gcggaatgac aggaaggatt gcctgagcag gcatgtccgc agtagcgggg accgcatagg
     2401 ccgctcccat cgtagggatc gctgcacctg gtgcttgccc cggcatcgca gtttgagcgg
     2461 gcgcccctgg agtggctgca taggcggaag gtgccgacgc gggtgaggaa gctggcgcgg
     2521 acgctgatgg ggaagcgggg gcagaacccg gagaagacgt cgcataacca cccgaaggtg
     2581 aagtatgttg aactgagatc gcggaaacgg cagcaaccga ctctgcgatt ttctgtacga
     2641 tcgaatcaga tagattcttg atgccctctg ttagattttc cagtgccagg ttctggagag
     2701 tcagagggat tacgatttct ggaaatttga gttggtcgtc ttcgctttca aatccggaaa
     2761 gccatctgcg aatatcgtct atgttggtct ggatgcgcga ctgaatgtcc gcatcctgaa
     2821 ctcgattagc cactcgttcg aatacttcga gcgcctctgt tgccttttgc tttgatacga
     2881 gtatttcagc attttgcaga gctcgtctgt gatgcgcaaa tctagaggag cgtgcgatga
     2941 gttgatctgt gttgtatgtt acgcgaagag ggactttgga cgagcgagat ggctcctgtt
     3001 ttctggagac accctttgcc tggattttgt ccggagcttt tgcgagtaga tcgcggatct
     3061 ggtcggtggt gtccttgccg ccgagtggca cagcagtgcc tcggtcatct ttgactccag
     3121 gcaaaccggg actggaggat ggcccttccg agcttccctg ttcccccggc gagggacgaa
     3181 cggcccacag acccacaccc gcggctacgc cgccggccaa tagtaaaagt aaccactcca
     3241 taaaaagcgt cttgtcctga ttatcgaccc cggaagcttc agtatccaac gcctctggcg
     3301 atcaactcat ggctttgccc attcagaaaa tcaactccag gctggagggc gaattcgttt
     3361 ttcgcggaag accctacagc gttccgttta ctttgccggg ggatctggtg cagttcaagc
     3421 tatcccggaa aaccgggcgg cttggaatgg aagtgcttca cattgagaag gcccacaaca
     3481 cagattttgc gctggcggat cctttttgtg cagtgtatgg aaggtgcggc ggttgcaggg
     3541 gccagcacat cccttatcca aagcagatcg aactaaaaac cacggacctg gtgcgagcca
     3601 tggaagggct cggtaagaag cctgagatcc tggttgctcc caggcttcag gggcatcgca
     3661 atcgaatgga tttcgtagtg gatgcaggag tcacaggatt ccgcgaggcg cgagagtttg
     3721 cgcgttttgt ggacgtcgat ttatgtcccg cagagagaga tcagtccaat cgtgttctag
     3781 aagtattccg gtctgcgatc gagcgccaca aggacatcgc gttcattcgc gaaagcggag
     3841 aagggatagt gaaatacgcc accattcgct ccggttctac ttcgcttctt tctataacaa
     3901 tagaagcgag tcgctcagaa aatcaacgtt atctggcatt cctgcaggat ctcatccaga
     3961 ccttgcctcc gcaatgctct cttgtggaaa ctcacactgt ttatccgtcg gacgtatcga
     4021 atccttccgg tggcaaggtt catgtgggag atggaacgta tacggatcgc ctgggcggtc
     4081 ttgattttcg tgtggcggct gattctttct ttcaacccaa tccggaagcg tttgatcttc
     4141 tcctggatca tgtgatggcc tggtttggtc cgggagatgt agcaactgaa aggcttgtgg
     4201 atttgtactg cggcaccgca gttctcagcg cgatttttac gaatcgcttt ccaggagact
     4261 ttgcgtctgt atacggcgcc gactttactg agtctgccat cgcacatgcg ccaggaaatt
     4321 tgaaacactt ttctgatccc gtcagattcg aggcactcga tttagctcaa aagatggttc
     4381 cgcttcttcc tggtgatttt gtaattcttg atcctcctcg cgccggtcta gctcctcagg
     4441 tcctgcgctc tgttctagat ggacttcctg agagagtgtt gtatatttcc tgcaatccac
     4501 aaacgcagct tcgcgatctt cgcgcattga cttctactta ccgaattgat tctgcggtga
     4561 tttcagactg ctacccccac acccagcatc tggagcaggc agtgtttttg aggaaactat
     4621 gagcgactcc gtattctcag gattgtggcg aagagcaaat tccggtgcgg aaaactgggt
     4681 caacgttctt gagatcaagc aagacaatga tcatatcacc ggcaagctac ggttatacga
     4741 aaagcaaccc ggaggagaag aggaaatact ctttgaatcg gatgtagagg gaagggtcgg
     4801 agcggagaat gcaaagatct atcttcgcac tcgtccactt cacgatgcca tatctctcta
     4861 tatagttgag agagatgacg atcggcttcg ccttttgccc atggggcatg acaacgatct
     4921 ggcaggagcc tgggtggctc aatgaatcga tagtagagga ttcgcgcttc cgtagtaatt
     4981 tggaagcttg ccatcccatt tcttaatcca ttccatatcg cgcatgagcg gtgttagagt
     5041 ttgtgtttgc agtctatacg cttctgcctg agcgcgcgct gttgtgattt gctgttctgc
     5101 ttctgtgcga attctttcca gatctctctg agctttcaga gccatctgct ctgcctgttg
     5161 cttggattcg attgcttcgt tgaacttctc agagaaattg aaattctcga cagagatggc
     5221 atccacaaaa aggaagaaag gcttcagtct atctgcgaga ttcttttgta tttcaaggct
     5281 tacgttttga cgatttgtga tcagttgctc tgcggtataa ttcgcggtga ctgcttttac
     5341 agtttctaaa atggcagggt caatgacagt tgcttcgtag tctgcgccca cattctgata
     5401 gaggtgattg acgtgcgctg gatcaatgtg atagttcact gcaatggtgg tttctacagt
     5461 ctgcaagtcg cgcgaggctg cctgcgcggt tgctttcgcc ttttgcacct gaacgttcat
     5521 ttctactacg gtctggtaga tcggcacctt gaagttgaat ccttctccca gtactctgtc
     5581 tgaaaccgct cccaggttcg tgaccacgcc acggtgaccc gctgagatca atgtgaacgg
     5641 ttttaagatc agcgccagga tcacgatgcc gccgatgatg gcccccttct tgagaaggct
     5701 gcccccttct gaactctgta ccatttcctt gagattcgcc atacaggaat cctaaccgcc
     5761 ccggaagaga tgcaagcgat aactggtgaa aacgttcgaa aactcatgac atttcgcggc
     5821 attccgtcgt gcgaaaaccg ttgtcaggcg cgcgcagtgc agtggaatca gccatcctcc
     5881 gatgatgtta gcagggactc tatgaaattg cgcctaccga ccgaaaagac atacagattc
     5941 agtattctcc aggcactgct ccctattgtg atcatatcta tcgtcgggat ttcgctcaac
     6001 ttcatcattg gctccactcg cgctgtagta gaactctccg atcatctggt agaagaagta
     6061 agccagagaa tgattgagag aactctagcc catctcgaag gcggcgcgat gcacaatcgc
     6121 gtgaccgcaa atcttgtttc tgatctagac ctgatgaaca accacgagac agtgtggcga
     6181 tatctctggg cgcccatgat cgaaacacct caggttcaga gtctgttcgt cgctgatcga
     6241 aggggaaact acgtgcaggt gcgtcgcgaa ccttttctgg ccactcgcat gattgatgct
     6301 gcgcgcggta tcgaaactac agtcactcgc gatgaatcat atcgcgaaaa agaaacgaag
     6361 acaaatccaa ctacctggga tcctcgcacc aggccctggt ataagaatac taccagggaa
     6421 aagaaaatgg cctggacgga tgtgttcgtc tttacgactg cgaagacacc cggtctttcc
     6481 gcttcttatc cagttctgga tgcacaggga gaaatcgtag gagtcatcgc ggccaatatc
     6541 cctctgcaca acctgtccga ttttgtagcg gaacagaagg tgatgcagca tggaattgtg
     6601 ctgatcatca acgacaaagg ggaggtcgtc gcctacccgg acaagaaagt gaatctggtc
     6661 gtggactcag atgcaaaaga gccggatcca aaagaacaca aagaaaaaga aatgcgcctt
     6721 cttcgcattg atgaactgcc aaatcaggcg gttgtagatg cgtatcgcag gtcggagagt
     6781 gaaaagagca ctcgcgtaac atctgcgaca aatggtgaaa aatacattac tacttttaaa
     6841 ccattcccgg cgccctttga aaaattgaag attgtgattg tgattccgga ggcggacatc
     6901 cttgcatcca ttcacagaat ggagctgatc gcgattctcg tgtctgtagt gattctgatt
     6961 ctgggcatca tcgttgtgta tcaggtgtcg attcgcattt cagatccaat cattcgtctt
     7021 gctcacgaga tggatcacat tggcgatttc agtctggatg atgtcaaagg agttcagtcc
     7081 aaaattcatg aaatcagaat tatgaatagc tcccttctga cagcggtgga aggattgaaa
     7141 gcgtttcgca ggtacttgcc agcgtccttc gtgcgtcagc tgattacttc cggcaagaag
     7201 gcagagctcg gcggaatcaa gactgagatc actctatttt tctctgacgt ggaagggttt
     7261 actagaattt ctgaaagcat gcctgcggaa gatctcatgc ttcatctgtc cgcgtatttt
     7321 aacgaacttt cgcgcatcat cctggaggag ggcggaacca ttgataaata cattggggat
     7381 gccatcatgg tgttctgggg acaaccggtt gaaatcgcgg atgcacccat tcgagcctgt
     7441 cgagcagctc ttcgttgcgt agagagactg acagagctca acgagaaatg gagtgcagaa
     7501 ggcaagccaa ccatgagaac tcgctttgca attcatacag gggaaagcat cgtaggcaac
     7561 gttggcacag aagagagaat gaactacagc gcagtgggtg atagcgtgaa cctgacttct
     7621 agactggaaa gcgccaacaa gatttacgga accaccatca tcatcagcca gagtacattc
     7681 gaacgagttc agcatttgtt tcgctgccgc cttctcgatg tggtggcggt gcgcggaaag
     7741 acacgcggag ttcgcattta cgagttgtta ggcgaatcta ccgtatctcc aacggaagcg
     7801 caaaatcgat ttgttgcagg ctacgaacag gcattttccg tctatctgag tcggaactgg
     7861 caggaagcag tgcaggcatt ctctaaactg aatgtggaat ttccggcgga taagtctgtc
     7921 caggtaatgc ttcgccgctg cgatgcattt gccaaggatg cgcccggtct
//
LOCUS       DAORFF010000107         7944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4491_length_7944_cov_4.203828, whole genome shotgun sequence.
ACCESSION   DAORFF010000107 DAORFF010000000
VERSION     DAORFF010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4491_length_7944_cov_4.203828"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..2574)
                     /locus_tag="PKK76_06015"
     CDS             complement(118..2574)
                     /locus_tag="PKK76_06015"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNL68059.1"
                     /translation="MKKVSGSKGLGSSIAQRKTTSVLRKYMPLLLIVAFFAPFSIFAS
                     EADLKIPDLSSVNFAIGNGKTLLFVGMGICVLGMLFGLMEFTAIKNLPVHIAMREISE
                     LIYETCKTYLLQQGKLLLVLEGFIGVIMVVYFGFLRHMPATTVLVILLCSLIGIAGSY
                     GVAWFGIRINTFANSRTAFAALRGKPFPNYAIPLRAGMSIGLLLISIELFLMLCILLL
                     IPAEYAGPCFIGFAIGESLGASALRIAGGIFTKIADIGSDLMKIVFKIKEDDARNPGV
                     IADCTGDNAGDSVGPTADGFETYGVTGVALISFILLAVKDPTVQASLLVWIFVMRIVM
                     ILASSGSYGINALIAKARYGNADDMNYEHPLTVLVWLTSFVSVALTYVVSYVLIPNLG
                     GDTTIWWKLSSIITCGTLAGAIIPELVKVFTSTESGHVREVVSASKEGGASLNILSGL
                     TAGNFSAYWMGITIAALMAGAYGISTMGIEGPLGMMAPAVFAFGLVAFGFLGMGPVTI
                     AVDSYGPVTDNAQSVFELSLIETRKGAREEIKKEFGFDPHFIKGKEFLEKNDGAGNTF
                     KATAKPVLIGTAVVGATTMIFSIIVILTKGLQPGLLANLSVLHPPFLLGLIVGGAVIY
                     WFSGASTQAVTTGAHRAVEFIKENIKLEGVEKASVEDSKKVVEICTIYAQKGMFNIFL
                     VVFFSTLAFSFVGEYFFIGYLISIAVFGLYQAIFMANAGGAWDNAKKLVETELRAKGT
                     PLHDASVVGDTVGDPFKDTSSVAMNPIIKFTTLFGLLAVELAIETPDTVRLALAGVFF
                     IVSTVFVWRSFYAMRIEQKG"
     gene            2683..3996
                     /locus_tag="PKK76_06020"
     CDS             2683..3996
                     /locus_tag="PKK76_06020"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNL68060.1"
                     /translation="MAEGGISLSNAWILPFVIFLLCVAIIPLINHHFWDRHLWWFSLG
                     FFCFPMVYALVFILGGPFIQKTVEKCMEYVSFMILLASLYVISGGILIRGHFRGTPLV
                     NTCFLVVGSVFASLIGTTGASMLLIRPLLRANAGRKRTAHIVVFFIFTVSNIGGLLTP
                     LGDPPLFLGYLQGVPFLWTIQLWKEWLFVLTLVIALFWLVDAYILRKDGKGIDLHHHD
                     TTEKFHISGVLNLILLLGVVASIFFHGYFLRTVSGWPHFGPQEGSMLVLTCVSLLAMP
                     VSHEIRLQNGFSWKPILEVMFLFAAIFATMIPALHILETRATSLGISAPWQFFLASGL
                     LSSLLDNAPTYLAFLSVAKGLALANDAGFVFHDGTHVSAAILAAISCGSVFMGANTYI
                     GNGPNFMVRAIAAERGVKMPGFFGYMGYSAAILLPIFALVAWIFF"
     gene            complement(4017..5054)
                     /locus_tag="PKK76_06025"
     CDS             complement(4017..5054)
                     /locus_tag="PKK76_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68061.1"
                     /translation="MTAQSRLPGIPLMRLLAICFFLTTTALAARDPEGLRLRASIFGG
                     GYTPDLQEKYANDRLYGSFVAGGIGANAPWNNKNASGLPLEAEYSTHPFHFELEIASI
                     HSRPDYAGVSAVSLTSSTIRYDVQQYDTTNVELSRFTPVLLARYDLTSQDSAHEISIL
                     GGIRRVDTRATYDYKYAQRFTISQSGLSVSGSVYGQSFDFRSRGVGTGLIFGGDYRYH
                     LENGSNFKLRLALFSADGSWFQNRETADVNTGGSGSGLIRREDGGYHVNGVEFIAGYF
                     HSVSENIRIFAQLRTETSAIRDNKVRLTRISQPTATEFQNLLIDSALTYPGSHAKDTM
                     GGLQAGVEVIL"
     gene            5093..6085
                     /locus_tag="PKK76_06030"
     CDS             5093..6085
                     /locus_tag="PKK76_06030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL68062.1"
                     /translation="MTPEAITGFENRLIRMHKHIGKWARRQGITCFRIYDRDVPQFPL
                     CIDKYEDFIIVSEYRTSKTAEGDEHDQWIDLCRQIIAKTLSVPIAHIDFRMRERKKGS
                     SQYEKTDTAGMEQVVHEAGLKFQIQLSAYLDPGLFLDHRITRGMVREEAKGKRCLNLF
                     AYTGSFSVYCAAGGSASTTTVDLSRTYCTWAMRNFTLNDFAARIVFDRDHKEASDLLP
                     LKNGNLVLQRDVLDFVDDCPPSSFDLAVLDPPSFSNSKRMAASFDVQRDHSDLIRRVF
                     RLLSPGGILYFSNNLTTFELDTLPGLVAKEITSQTVPMDFRNKRPHRCYRILRK"
     gene            6158..6823
                     /locus_tag="PKK76_06035"
     CDS             6158..6823
                     /locus_tag="PKK76_06035"
                     /inference="COORDINATES: protein motif:HMM:NF016208.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68063.1"
                     /translation="MARLWLGPFAFLVSCAAAFPDNSAKTDTFAAIAGATLLRPGSVA
                     PHVSCDAGLQNSSGQAGTLANWTTLLNGGSGWAVVNFSFANPGFGTSFSTDSKYQEID
                     LVANGFSPGTLDTAPTVYFGERFLKRMDVTGINYYLKVEIRNAAHAAMQTYNSGMLNA
                     VPGMGADVVYPLTSSFSGYGAGMRFIYVEDGGYDTMSWAGQFGPYLHSAYVRLSNACG
                     LPY"
     gene            complement(6825..7748)
                     /locus_tag="PKK76_06040"
     CDS             complement(6825..7748)
                     /locus_tag="PKK76_06040"
                     /inference="COORDINATES: protein motif:HMM:NF010612.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNL68064.1"
                     /translation="MEIGAIIITIAGLCLFETVSSIDNAIINAEVLGTMKPKYRRWFL
                     IYGIFIAVIVVRGALPWLIVWAVNPTLGPVGAFMASFSNDPHVVDSIQASAPILLVGG
                     GTFLILLFLHWLFLEPKNYGLYGERYIESQGVWFYAFASIALLVIVWLALKKDSMMAM
                     GAVTGSTAFFITHGFKENAEQAEQDLLTSHKSDIAKILYLEILDATFSIDGVLGAFAF
                     TLYVPLILIGNGLGAIVVRQLTVGNIERIKKYKYLKNGAMYSILCLGVVMVLESFAVH
                     VPSWLSPVVTFVVVGIFFVRSVYAIRAEEAE"
     gene            complement(7739..>7944)
                     /locus_tag="PKK76_06045"
     CDS             complement(7739..>7944)
                     /locus_tag="PKK76_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNL68065.1"
                     /translation="FKTNRWFRRPVFKRYATPVEAKRGDELGRFEMGSTVILLFGHNK
                     IDFLPIIQEKNKVKFGQGIAKWK"
BASE COUNT         2099 a         2001 c         1925 g         1919 t
ORIGIN      
        1 ccgccattat gcgatcctca ttcacccatt tcgggttgcc gggccaaaaa aaaacccggg
       61 gaaaccccgg gttttgcaga cctccccaaa aagaaggtcc tattcagaat ccgccattta
      121 gcctttctgt tcgattcgca tcgcgtagaa agatctccac acaaatacag ttgaaacaat
      181 aaagaacacg cctgcgagag ccagacgaac tgtgtccgga gtttcgattg cgagttcaac
      241 agcaagcaga ccaaacagag tggtgaactt gatgatcggg ttcattgcaa cagaagatgt
      301 gtctttgaaa gggtcaccta cagtgtcgcc cacaacagac gcgtcatgca gaggagttcc
      361 cttcgcgcgg agttctgttt ccacgagttt cttcgcgttg tcccatgcac caccagcgtt
      421 tgccatgaaa atagcctgat acagaccaaa cacagcaatg gagatgaggt atccaatgaa
      481 gaagtattct ccaacgaatg agaaagcaag tgtgctgaag aacacaacga ggaagatgtt
      541 gaacatacct ttctgagcgt agatggtgca aatttcgacg actttcttgg agtcttctac
      601 ggatgctttt tccacgcctt cgagcttaat gttctctttg atgaactcaa ccgcacgatg
      661 cgcaccagtt gtaactgctt gcgtggacgc gccagagaac cagtagatca ccgcgccgcc
      721 gacaatgagt ccgagcagga atggtggatg cagaaccgag aggttcgcaa gcagaccagg
      781 ctgcaggcct tttgtcagaa tcacaatgat tgagaaaatc atagtagttg cgcccacgac
      841 agcggttcca atcagaactg gcttcgcagt tgctttgaaa gtgttacccg cgccgtcatt
      901 cttctcaagg aattcttttc ctttgatgaa atgaggatcg aatccaaatt cttttttgat
      961 ctcttcgcga gcgcccttgc gcgtttcgat gagagaaagt tcaaatacag actgagcgtt
     1021 gtcagtaacc gggccgtaag agtctacagc gatggttaca ggacccattc caaggaaacc
     1081 aaaagctacc agaccaaagg cgaaaacagc aggagccatc atgccgagcg gtccttcgat
     1141 tcccatggtg ctgattccat atgcgccagc catcagagct gcgatcgtga ttcccatcca
     1201 gtatgcgctg aagttaccag cagtcagacc ggaaagaatg ttcagagatg cgccgccttc
     1261 tttagaagca gatacaactt cgcgcacgtg accggattca gtagacgtga acactttcac
     1321 gagttctgga atgatcgcac ctgcgagtgt accgcaagta atgatagaag aaagtttcca
     1381 ccagatcgtg gtatcaccac cgaggttcgg gatgagcacg taagaaacaa catatgtcag
     1441 agcgacagaa acgaaagaag tcagccatac aagaactgta agcggatgct cgtagttcat
     1501 gtcgtctgcg tttccgtaac gcgctttcgc aatcaatgcg ttgattccgt aagaaccgga
     1561 agaagcgagg atcatcacaa tgcgcattac gaagatccaa acgagcagag atgcctgaac
     1621 ggttgggtct tttactgcaa gcagaatgaa agaaatgaga gcaacacctg ttacaccata
     1681 agtttcgaat ccgtcagcag ttggaccaac tgagtcacct gcgttgtcgc ctgtgcagtc
     1741 tgcaatcaca cccgggttac gggcatcgtc ttctttaatt ttgaaaacga tcttcatgag
     1801 gtcggaaccg atgtccgcga tcttggtgaa gataccgccc gcgattctca gagcagatgc
     1861 tccaagtgat tcaccgattg caaaaccgat gaagcaagga ccagcgtatt cagcaggaat
     1921 caacagaaga atgcagagca tcaggaacag ttcgattgaa atcaggagga gaccaatgct
     1981 catacctgct ctcagtggaa ttgcgtagtt aggaaatggc ttaccgcgaa gcgccgcgaa
     2041 tgcggtgcga gagtttgcaa acgtgttgat acgaatacca aaccatgcaa caccgtaaga
     2101 acctgcgata ccgatcaagc tgcaaagcag aatgacgagc accgtagttg ccggcatatg
     2161 gcgcaggaaa ccaaagtaaa ccaccatgat tacgccaatg aaaccttcga gaaccaggag
     2221 cagtttgcct tgctgaagca gatacgtttt acacgtttca tagatcagct cggagatttc
     2281 gcgcatagcg atgtgcactg gcagattctt gatcgcagtg aactccatca gaccgaacag
     2341 cataccgaga acacaaatac ccatgcctac gaaaagcagg gtcttcccat ttccgattgc
     2401 aaagttaacg gatgacagat ctggaatttt aagatccgct tcgctcgcaa aaatcgagaa
     2461 gggagcaaag aacgctacta taaggagcag gggcatatac ttccgcagta cggaagttgt
     2521 cttgcgctga gcgatagacg agcccaagcc ttttgagcct gaaactttct tcattttgaa
     2581 cctcttaaga aattgagtaa gagccgattc ggatcttacg gatgcaaaat gtgcatttta
     2641 agattgactc atcgatgcgc gaaaactgac aactctaatt tgatggcaga aggcggaatt
     2701 tccctatcga atgcgtggat tttgccgttt gtgatctttc tgctctgcgt tgccatcatt
     2761 ccactgatca accatcactt ctgggatcgc catctatggt ggttttctct tggtttcttt
     2821 tgttttccca tggtctacgc tcttgttttc atcctgggag gaccgttcat tcagaaaaca
     2881 gttgagaaat gcatggaata tgtctcattc atgattctgc ttgcgtcgtt gtacgtgatt
     2941 tcaggcggga tcctgattcg aggacacttt cgcggaacac ctctcgtcaa tacctgcttc
     3001 cttgtggtgg gttcggtatt tgcaagtttg attggaacaa ccggtgcatc catgcttctg
     3061 attcgtccgc ttttgcgcgc aaatgcaggg cgaaagcgga cagcccatat agttgtgttt
     3121 tttatattta cggtttctaa cattggaggc ctgctcactc cactcggtga ccctcctctg
     3181 tttctgggtt atctgcaggg tgttcctttc ctgtggacca ttcagctctg gaaagaatgg
     3241 ttgttcgttc ttacactcgt gatcgcctta ttctggctag tcgacgcgta cattctgcga
     3301 aaggatggaa agggaatcga tcttcatcac cacgatacaa ccgagaagtt tcacatatcc
     3361 ggcgtgctga acctgattct tctgttagga gtggttgctt ccattttctt ccacggctat
     3421 tttctgcgaa cggtctccgg ttggcctcac tttgggccgc aggaaggatc catgctagtg
     3481 ttaacgtgcg tctctctcct ggcgatgcca gtgagccatg agatacgcct gcaaaacggt
     3541 ttcagctgga agccaattct agaagtcatg tttctattcg ctgccatctt cgcgaccatg
     3601 attcctgcgc tgcatatcct cgagaccagg gcgacatcgc tcggcatcag tgctccgtgg
     3661 cagttcttcc tcgcttccgg cctgttgtct tctctgctag acaatgcgcc aacgtatctc
     3721 gcttttctgt ccgtcgccaa ggggctcgca cttgccaacg atgctggttt cgtatttcac
     3781 gatggcacgc atgtaagcgc cgcgattctg gctgctattt cgtgcggttc ggtgttcatg
     3841 ggcgcaaaca cttacattgg aaatggtccc aacttcatgg tcagagcaat tgcggcggaa
     3901 cgaggagtga agatgcccgg cttctttggc tacatgggat attcagccgc catccttctc
     3961 ccgatcttcg cactggttgc ctggatattc ttctagtcgc ttcgcaacct ttgtatctac
     4021 aggatcactt ctactcctgc ctgaagtccg cccatcgtat ccttcgcatg gcttccggga
     4081 taggtcagtg cagaatcaat gagcaagttt tgaaactctg tagcggttgg ctgagagatg
     4141 cgcgttagac gcactttgtt gtctcgaata gcggaagtct ctgtgcgaag ttgagcaaag
     4201 atgcgaatgt tctctgaaac agaatgaaag tatcccgcaa tgaattctac gccatttaca
     4261 tgatagccgc cgtcttcgcg tcgaatgagc ccgctcccag agccacccgt attgacgtca
     4321 gctgtttctc tattctgaaa ccaggatcca tctgcagaaa aaagcgcaag cctgagtttg
     4381 aagttcgatc cgttctcaag atgataacga tagtcgccac cgaagatcaa accagtgccc
     4441 actccgcgcg aacgaaaatc aaaactctgc ccgtatactg atccagagac agagagaccg
     4501 gactgcgaaa ttgtaaacct ctgggcatat ttataatcgt aagtggctcg agtgtccacg
     4561 cgtctgattc ccccaaggat cgaaatctca tgcgctgaat cttgcgatgt taaatcgtaa
     4621 cgtgcgagaa gaaccggagt aaatctgctg agttctacgt ttgtcgtatc gtactgctga
     4681 acatcgtaac gaatcgtaga cgaagtcaag ctcacagcag aaacacccgc atagtccggg
     4741 cgagagtgaa tggacgctat ttcaagctca aaatgaaacg gatgggtaga gtattctgct
     4801 tcgagcggca acccggatgc attcttgttg ttccagggtg cgttggcccc gatcccgccg
     4861 gccacaaagg aaccatagag acgatcattc gcgtacttct cctgcaaatc aggagtgtag
     4921 cccccgccaa aaatcgacgc gcgaagacga agcccttccg gatctcgagc cgcaagtgcc
     4981 gtcgtggtga gaaagaaaca aattgcaaga agcctcatga gtggaatacc gggcaaacgg
     5041 ctttgcgcgg tcaagcgatc gccggaaaag accgcaacga tcttgaatca aaatgacacc
     5101 agaagcaatc acaggatttg aaaaccgtct gatccgaatg cacaaacaca ttggcaagtg
     5161 ggcccgcaga cagggaatta cctgttttcg catctacgat cgtgatgttc ctcaattccc
     5221 gctctgcatc gacaaatacg aagattttat catcgtttca gaatatagaa ccagcaagac
     5281 ggcagaggga gatgaacacg atcagtggat cgacctgtgt cgtcagatca tagcaaagac
     5341 tctgtctgtt cccatcgcac atatagattt tcggatgcgc gaaagaaaga agggaagctc
     5401 tcagtacgag aaaacggaca cagcagggat ggaacaggta gtgcatgagg caggtttgaa
     5461 atttcaaatc cagctatccg catacctgga ccctgggctc ttcctggacc acagaatcac
     5521 gcgcggtatg gttcgcgaag aggcaaaggg gaagcgttgc cttaacctat tcgcttatac
     5581 cggatctttc agtgtgtact gcgcagcagg cggctccgcc tccacaacca cagtagacct
     5641 ctctcgcaca tattgtacct gggccatgcg taatttcacg ttaaacgact ttgcggccag
     5701 aattgtgttc gaccgtgatc ataaagaggc aagtgatttg cttccattga agaacggcaa
     5761 tctggtgctc cagagagatg tgctcgattt tgtagacgat tgtcctcctt cgagctttga
     5821 tctggcggtg cttgatccac ccagcttctc aaacagcaag aggatggctg caagttttga
     5881 cgttcaacgg gaccactcgg atctgatccg aagagtattc cgcctgcttt caccgggcgg
     5941 aatactatat ttctctaaca atctgaccac cttcgaactg gatacacttc ccggacttgt
     6001 agcaaaagaa atcacgtcgc aaactgtccc catggatttt cgaaataaac gcccgcacag
     6061 atgctatagg attttaagga aatagccggc atcctcgcac agcttctagc tcgatttttt
     6121 tctcgcaaac cagaatgcag ggtctaaggt gtggcctatg gcgcgtctct ggcttggccc
     6181 atttgcgttc ctggtgagct gcgctgctgc ctttccggat aacagcgcga agacggatac
     6241 gtttgcggct atcgccggtg ccacattgct caggccaggc agcgtcgcac cccatgtatc
     6301 ctgcgacgca ggattacaaa attcaagcgg tcaggcagga actcttgcaa actggaccac
     6361 acttctaaac ggaggaagtg gttgggcagt cgtcaacttt agcttcgcaa accctggatt
     6421 cggcacttcg ttttccacgg acagcaagta tcaggaaatc gatcttgtag caaatgggtt
     6481 ttcccctgga actctggata ccgcacccac agtctatttc ggagaacgat ttctaaaacg
     6541 catggacgta actggaatca attactacct gaaagtggaa atccgaaatg cggcacatgc
     6601 cgccatgcaa acatacaatt ctggaatgct gaatgccgtt ccgggaatgg gtgcggatgt
     6661 cgtttatcca cttacgtcca gtttctcagg atacggcgcg ggaatgcgct tcatctatgt
     6721 ggaggatggt ggatatgaca caatgagctg ggccggacaa ttcggcccgt atcttcattc
     6781 cgcatacgtt cgtctttcga atgcatgcgg cttgccttac tgaatcattc tgcttcttct
     6841 gcgcgaattg cgtaaacaga acgaacaaaa aagatcccga ccacaacaaa cgtcacaacc
     6901 ggcgaaagcc acgagggcac gtgaaccgcg aaggattcga gaaccattac gactcccagg
     6961 cagagaatgg aatacatagc accatttttc agatacttgt acttcttgat tctctcgatg
     7021 ttcccaacag tcaactggcg cactacaatg gcacccaggc cattgccaat caggatcaat
     7081 ggaacataca gcgtgaaagc gaaagcacct aacaccccgt caatggagaa ggttgcgtcc
     7141 agaatttcca aatagaggat cttcgcaatg tctgatttgt gcgaagtaag caaatcctgc
     7201 tctgcctgct ccgcgttctc tttaaaaccg tgggtgataa aaaaagcagt cgagcctgta
     7261 accgcaccca tggccatcat ggaatccttc ttcagtgcaa gccagactat aacgagaagt
     7321 gcaatcgacg cgaatgcgta aaaccagaca ccttgcgatt caatgtatct ctctccataa
     7381 aggccatagt tctttggctc aagaaacaac cagtgcaaaa acaacaggat gagaaatgtt
     7441 ccgccgccta ccaggagaat gggggcagag gcctggatgg aatccacaac atgtggatca
     7501 ttcgaaaacg aagccatgaa tgcgcccact ggaccaaggg ttggattcac ggcccacaca
     7561 atgagccagg gcagagcgcc gcggaccacg atgacagcaa tgaaaatgcc ataaatcaaa
     7621 aaccatcggc gatactttgg cttcatcgtg ccaagcacct ctgcattgat gatggcattg
     7681 tctatgctgg aaaccgtctc aaaaagacag agacccgcaa tggtaatgat gatcgcgcct
     7741 atttccattt tgcaatcccc tgtccaaact tgactttgtt cttttcctgg atgattggta
     7801 agaagtctat cttgttgtgc ccgaacaata ggatcactgt ggaccccatt tcaaatcgac
     7861 caagctcatc tccgcgtttc gcctccacgg gtgtcgcata acgtttgaac acaggacgac
     7921 ggaaccagcg atttgtctta aatg
//
LOCUS       DAORFF010000108         7935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4506_length_7935_cov_4.300761, whole genome shotgun sequence.
ACCESSION   DAORFF010000108 DAORFF010000000
VERSION     DAORFF010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4506_length_7935_cov_4.300761"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="PKK76_06050"
     CDS             <1..1168
                     /locus_tag="PKK76_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNL68066.1"
                     /translation="QGSTDMSWNERCIRQADKILLVADAGQDPRLAPVELKYPPDAEH
                     VSGERNLVLIQPADALRAHNTKDFLANRGNMRHFHVRRNSSADFQRLARGLTGNSIGL
                     VLGGGGARGMAHIGVLKALIEHQVPIDMVGGTSAGAIMAGTFAFFQDLERVSITVREF
                     MVDRNPLNDYTFPFLSLARGRKYSEALERVFGNVSLEDLLLPAFAIACNLSTSQEACL
                     KSGAIWTALRATSSLPGIAPPLFMDGQLFVDGGLLNNLPVDAMQDFGAGKIIAIDVSS
                     NAQHEDEEYGKFMGAQTASVGPSFFRVLANKLRPRARRYKLPSLASVLIRSTMIGSVK
                     RVRNARREADIYARLPLGGFGLLDWKAMDDLIKIGYDYAVENMPLWKKSLGLSD"
     gene            1183..1581
                     /locus_tag="PKK76_06055"
     CDS             1183..1581
                     /locus_tag="PKK76_06055"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNL68067.1"
                     /translation="MREPRGFTFPSLITGITSAMLLIDLAGLVSHFNVDLRMGFLAYV
                     FIGTETHRMHHSADTISSKNFGNTLALWDIVFGTFQYASGQRPERIGIADPETHPRSE
                     DLFRVLAFPFGGPPGLAEKEVHPLDEGASV"
     gene            1585..1833
                     /locus_tag="PKK76_06060"
     CDS             1585..1833
                     /locus_tag="PKK76_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68068.1"
                     /translation="MKSYKFTLSYPEKKSPALENKIGPLVRKHNGKFESKFNFADNET
                     IVTVTLPKADKAGFEADLKDPKYNFLHLESVVFAMDEE"
     gene            1830..3110
                     /locus_tag="PKK76_06065"
     CDS             1830..3110
                     /locus_tag="PKK76_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tudor-knot domain-containing protein"
                     /protein_id="HNL68069.1"
                     /translation="MKLFIKFSISWATHSRFSFVWITVLSFWIAAPLRAEEWNFSTTR
                     GVVAGVLKWKDSGLESFSDRFRKDAELHSLLIRRGVPASNLKLLLDGAATRAGIESAV
                     REAALNAPADSVFYFYYAGHGIKDASGSYLANYDIDSGSPESTGVNIRRIAEILASSF
                     KGKMVIFTGDFCYSGAFEEALRTLQKKGIRGFMLASASASNESTGNWTFSQTWIDCLS
                     GNPLCDKNGDGKITLGEAQSEVAQAMKFRERQRNGFLLAGVSESLIISSATGKNQPGV
                     GSYVLAPRAGAMLPARITNRNGSRVTCEFYSYSKKTSVDLAENAVKPLNFRQYSVGTN
                     LKVIWNGKSYPATVKKLDNDFHLITYSGYDSSWDEWVMDDRIVADDSVLVEWKGKWYP
                     AKVLKTEGGRSFIHYEGFESSWDEWVTSSRIKKP"
     gene            3162..4640
                     /locus_tag="PKK76_06070"
     CDS             3162..4640
                     /locus_tag="PKK76_06070"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006969.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HNL68070.1"
                     /translation="MSDLNGVMMQYFQWYSPADGSYWKQTAANARELAAAGITALWLP
                     PAYKGIGGGADVGYGVYDMYDLGEFNQKGSVRTKYGTKEEYLSCIQTLQQSGIQVYAD
                     TVLNHRMGGDNPETPVATPFWQDNRMYAKGEPRKIQAYTHFQFPGRAKKYSDFEWHWN
                     HFDAVDYDGFNPNDHSTVYLLEGKQFDDRVAMEKGNFAYLMGCDLDCQSADVRAELIR
                     WGKWYLDTTKVDGFRLDAIKHIAAWFFPEWLDTLEKYVNKDLFVVGEYWVPDVEALHN
                     YLNLVGERMTVFDVPLHFNFHAASKSGGNFDMRRLFSGTLMEQRPRMAVTFVDNHDSQ
                     PLQALESCIEPWFKPLAYAAILLRREGYPCIFHPDYYGADYEDRGKDGNTYRIVMPSH
                     RFLIDKFLYARRNFAYGPQYDYFDHYNSIGWTRLGDLSHPKSMAVLMSDGPAGHKWME
                     TGKRGAQFRDITGHVSHIVTTNGDGWGEFHCNGGSVSVWVEE"
     gene            4666..5628
                     /locus_tag="PKK76_06075"
     CDS             4666..5628
                     /locus_tag="PKK76_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNL68071.1"
                     /translation="MMKKIVLIVLGLIVGSVVGLLGYVRFALPNVGPAPVMTIESTPE
                     RVERGKYLANHVTVCIDCHSTRDWSKFSGPLVPGAIGKGGELFDQKFGFPGAYYSRNI
                     TPAGIKNWTDGELFRLITTGVTKDGKAIFPVMPYPYYGRMDEEDIKSIIAYVRTLPAL
                     ENEVPASRSDFPMNFIVNTIPHKASLVKRPDPKDEVKTGEYLANAGACMECHTQVEKG
                     QVIPSLAYAGGREFPMPTGDMVRSANITPDVETGIGKMTRKEFVARFKERMAAARAPA
                     QGFNTIMPWGMYGGMTDQDLGAIYEYLRTISPKANNVVKYTPAK"
     gene            complement(5651..6901)
                     /locus_tag="PKK76_06080"
     CDS             complement(5651..6901)
                     /locus_tag="PKK76_06080"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNL68072.1"
                     /translation="MIDFGFDRFWKLTTAAGRLGWALLGDPTEQRRLKSAARAIESLS
                     KLKGAPMKLGQMLSLHEGLLPPEISGLLAGLRKDAEPIAVSEIQDILRLELGEEKASQ
                     LRLEEKPIAAASLGQVHRASHPDGRVFAIKVQYPGIREALGSDLRILRTIMTAFSPMT
                     AVPARKLIGEVKDRLLEEIDYVREASHLDQATEMYSDPIVIPTPVREFCTGRVLTTNF
                     IPSMSIAEASVSAETLRFQWAVHLLTAIVKGAVEHKFLHADPNAANVGFLSDGRIVLY
                     DFGCVKLVPESLSDSYSKAAAELLSGRLSPVPGLLFAAGIRYKSGEKLTEEFIQPIAR
                     MLNQVFPDEETVFGSIPDIYSKLWQMGTSDFLESRNIVFPPDLIFVHRTLLGHFGNLR
                     ALKPRGNWRQIVLGLLADAGVRPD"
     gene            complement(6894..7895)
                     /locus_tag="PKK76_06085"
     CDS             complement(6894..7895)
                     /locus_tag="PKK76_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNL68073.1"
                     /translation="MGEAHKTSQSHVHLATSGMNAFFTVFRGLQEIQATKGRTLWLQL
                     GWLYVDTGEIIKKFTAPSDRAFMGDVFDMGAIRDFVSKNSDRIAGIVTECPTNPLVEM
                     TDLQELHAITRSAGAALILDPTLVTPLNVEVLVHSDAVINSLTKYASHMGDVMIGSAV
                     LNPASPFYKELSRKVETSIENPYPRDLARLAEQIENYHSVVAKINANTMRLATYLSNH
                     KNIKKVYWAYAEQSRKNYEKFQRRAEAPGGIITIELNIPLEKFYDRITVAKGPSFGSV
                     FTILCPFMYLAHYDLVQSEAGRRELASLHLDPDLVRVSVGIEPIEEIIAAFAEALSD"
BASE COUNT         1934 a         1954 c         2084 g         1963 t
ORIGIN      
        1 acagggaagc acggacatgt catggaacga gcgttgcatt cgtcaggcgg ataagatctt
       61 gcttgtcgcg gacgcaggcc aggaccccag gctcgctcct gtggaattga agtatccccc
      121 ggatgcggaa catgtttccg gcgaaagaaa tcttgtactg atccagccgg cagatgcttt
      181 gcgcgcgcat aacacaaagg actttctggc caatcgcggt aacatgcgtc attttcacgt
      241 taggcgaaat tcatctgccg attttcaaag gctggcacgc ggattgacag gaaattcaat
      301 cggactggtc ctgggcggcg gtggcgcgag aggaatggct cacataggag tgctgaaggc
      361 tctcatcgag caccaggtgc caatcgacat ggtgggtggt acaagcgccg gcgccattat
      421 ggccgggaca ttcgctttct ttcaagacct ggaaagagtt tccatcacag tgcgtgagtt
      481 catggtggat cgcaatccgc tcaacgacta cacgtttccg tttctctccc ttgcacgggg
      541 tcgaaaatac tcagaagctc ttgaacgggt attcggcaac gtgagcctgg aagatcttct
      601 gctgcctgct ttcgctattg cctgcaatct cagcacctcc caggaagcat gccttaagag
      661 cggagccatc tggactgctc tgcgcgcgac atcgtcgctg ccgggaatcg cgcctccgct
      721 gttcatggac ggccagctat ttgtggacgg gggtttgctg aacaaccttc cggtggatgc
      781 aatgcaggat tttggcgccg ggaaaatcat tgccatcgac gtttcatcca atgcccagca
      841 cgaggatgaa gaatatggaa aattcatggg cgcgcagact gcctcggtgg gaccgtcttt
      901 ctttcgcgtt cttgccaaca agcttcgacc cagggctcgg cgttacaaac ttccatcact
      961 cgccagcgtc ctgattcgat ctacaatgat tggcagcgtt aagagggttc gcaatgctcg
     1021 aagagaggcg gacatctacg cacgtctccc tctcggcggc tttggactgt tggactggaa
     1081 ggccatggac gatttgatta agataggtta tgactacgcg gtcgagaata tgccgctgtg
     1141 gaaaaaaagt cttggattga gtgactgaac agggcgactc acgtgcgtga accgcgcggc
     1201 ttcacgttcc cttctttgat cacaggcatt accagtgcca tgctactgat tgatctggcc
     1261 gggcttgttt ctcatttcaa tgtagatctt cgcatgggat ttcttgcata cgtattcatt
     1321 ggaaccgaga cacatcggat gcatcacagt gcggatacta tttcatccaa gaattttggg
     1381 aacacccttg cattgtggga cattgtgttt ggaacctttc aatacgcgtc agggcagaga
     1441 cctgagagga ttggaattgc cgatccagag actcatccgc gatcagaaga tctctttcgc
     1501 gtgcttgcct tccccttcgg tggacctcca ggattagcag aaaaagaagt gcatccgtta
     1561 gacgagggtg catccgtgtg aaacatgaag tcctataagt tcactctttc ctatccggaa
     1621 aagaaatctc ctgctctcga aaacaaaatt gggccgctgg ttcgcaagca caacggaaag
     1681 ttcgagagca agttcaattt cgctgacaat gaaaccatcg tgactgtcac tctaccgaaa
     1741 gcggacaaag ccggtttcga ggcagacctg aaagatccaa aatacaactt tctgcatctt
     1801 gaaagtgtag tgtttgccat ggacgaggaa tgaaattgtt tataaaattt agtatttcct
     1861 gggccacgca ttcccgtttc tcattcgtct ggattaccgt tttatcattc tggatcgccg
     1921 cgcctcttcg tgcggaagaa tggaattttt caaccactcg gggagtagtt gcgggagtgt
     1981 tgaagtggaa agattcagga ttggaaagtt tctcggatcg attccgcaaa gacgcagaat
     2041 tgcactcgct cttgattcgc cgcggcgttc ctgcatcgaa tttgaaactg ttgttagacg
     2101 gagccgccac acgcgcaggt attgagagtg cggtaaggga agcagccctg aacgcaccgg
     2161 cggacagcgt tttctatttt tactatgccg gacacggaat caaggatgct tcagggtcct
     2221 acctggccaa ctacgatatt gattctggca gccccgaatc tacaggcgta aacatacgtc
     2281 gcatcgcgga gatcctggcc tccagcttca aagggaagat ggtgatcttc acaggagact
     2341 tctgctattc aggtgcgttc gaagaagctc ttcgcactct gcagaagaaa ggaattcgcg
     2401 gtttcatgct tgcgtcggcc agcgcgtcca atgagtccac aggcaactgg actttttcgc
     2461 agacgtggat tgattgttta tccggcaacc cgttatgcga caagaacggt gacggaaaaa
     2521 tcactctggg agaagctcag tcagaagtgg cgcaggctat gaagtttcgt gagagacaga
     2581 gaaatggctt cttgcttgcc ggtgtcagcg aatctctcat catttcgtct gcgacaggaa
     2641 aaaatcaacc cggagtaggc tcctatgtgc tcgctccgcg cgcgggtgcc atgcttccgg
     2701 ctcgtatcac gaacagaaac ggatccaggg taacttgcga attctattcc tatagcaaaa
     2761 agacttctgt ggatctcgca gagaatgcgg tgaaaccttt aaactttcgt cagtattctg
     2821 tcggaaccaa tttgaaagtc atctggaatg gaaaatctta cccggccact gtcaagaagc
     2881 tggacaacga ttttcatttg attacgtatt ccggatacga ttcgtcgtgg gatgaatggg
     2941 tcatggacga tcgcattgtc gcagatgatt ctgtgctggt ggaatggaaa gggaagtggt
     3001 atcctgcgaa ggttttgaaa acagaaggag ggagatcgtt tatccattac gagggatttg
     3061 agtcttcctg ggatgaatgg gtgacgtctt cgcgcataaa gaaaccctga aacagtttac
     3121 atttctaaag ctttgttcct caatgcccaa ggagaatctt catgtcagac ttgaacggcg
     3181 ttatgatgca atacttccag tggtatagcc cggcggatgg cagttattgg aagcaaactg
     3241 ctgccaatgc tcgtgagctt gcggctgctg gaattaccgc tctctggctt cctcccgcat
     3301 acaagggaat cggtgggggg gcggacgttg gctacggcgt ctacgatatg tatgacctgg
     3361 gcgaattcaa ccagaaggga tccgttcgca caaagtacgg aacaaaagaa gagtatctct
     3421 catgcatcca gacactgcag cagtcaggca tccaggtata tgcggacaca gtactaaatc
     3481 atcgcatggg tggtgacaat cctgaaactc cagtggccac tccattctgg caggataaca
     3541 ggatgtacgc gaaaggagag ccacgcaaga tccaggcata cacacacttt caatttcccg
     3601 gcagagcgaa gaaatactcc gatttcgaat ggcactggaa tcactttgac gctgtagatt
     3661 atgacggctt caatcccaac gatcattcca cagtttatct tttggaaggc aaacagtttg
     3721 atgatcgcgt tgctatggaa aaaggaaact tcgcgtacct catgggatgc gacctggact
     3781 gccagagtgc ggacgttcgt gcagaattga ttcgatgggg caaatggtat ctggacacta
     3841 cgaaggtgga cgggtttcgc ctggatgcga tcaagcacat tgcagcctgg ttctttccgg
     3901 agtggctgga tactcttgag aagtatgtaa acaaagacct gttcgttgtg ggtgagtact
     3961 gggtgccaga cgtagaggcg cttcacaact atctgaatct ggtcggagag cgaatgactg
     4021 ttttcgacgt tccccttcat ttcaattttc acgcggccag taaatccggt ggcaattttg
     4081 atatgcgccg ccttttctcc ggcactctca tggagcagcg tccacggatg gcggttacgt
     4141 ttgtagacaa tcatgattcg cagcctcttc aggcccttga gtcctgcatt gagccctggt
     4201 ttaagcctct cgcctatgcc gcaattttgc ttcgccgcga aggctatcca tgcatctttc
     4261 atcctgacta ctatggcgca gactacgaag atcgaggcaa agacggcaat acgtatcgca
     4321 ttgtgatgcc ttcgcatcgc tttctcattg acaaatttct atatgcaagg cgcaacttcg
     4381 cttacggacc gcaatacgat tactttgatc actacaattc aattggttgg accagactcg
     4441 gggacctttc gcatccgaag agtatggcag ttctcatgag cgatggtccg gccggccaca
     4501 aatggatgga aaccggaaaa cgcggagcgc agtttcgtga tattaccgga catgtttcgc
     4561 acattgtgac cacaaatgga gatggctggg gcgagtttca ctgtaatggt ggatccgtct
     4621 cggtctgggt agaagagtaa cgttagacgg cattgaaaag ggggaatgat gaagaagata
     4681 gttctgattg tgctcggatt gatcgttgga tcagtagtgg gcctgctcgg ttatgtgcgt
     4741 tttgctttgc ccaacgtggg cccggcaccc gtgatgacca ttgaatctac accggagaga
     4801 gtcgagcgag gaaagtatct ggcaaaccac gtgactgtat gcatcgattg ccattccact
     4861 cgcgactggt ctaaattttc aggcccgctt gtacctggtg ccattggaaa aggcggagaa
     4921 ttattcgatc aaaagtttgg ttttcctggc gcgtactatt cgcgcaacat cactcccgct
     4981 ggaatcaaga actggacaga tggcgaattg tttcggctga tcaccacagg cgttacaaaa
     5041 gatggtaagg caatcttccc cgtgatgcct tatccgtact acggtcgcat ggacgaagaa
     5101 gacatcaaaa gcatcatcgc gtacgtgcgt actctgcctg ccctggaaaa tgaagtgccc
     5161 gcttccaggt cggacttccc aatgaatttc attgtaaata caattccaca caaagcaagt
     5221 ctcgtgaagc gccctgatcc taaggatgaa gtgaagacag gcgaatacct ggcaaatgcc
     5281 ggagcttgta tggaatgtca cactcaagtg gaaaaagggc aggtgattcc atctcttgct
     5341 tacgcaggtg gtcgcgagtt tccaatgcct accggcgata tggttcgttc tgccaacatt
     5401 actcctgatg tggaaactgg aattggcaaa atgaccagaa aggaattcgt cgcgcgattc
     5461 aaagagcgca tggccgcggc ccgcgcacct gctcaaggtt ttaatacaat tatgccgtgg
     5521 ggcatgtatg gcggcatgac agatcaggat ctgggagcca tctacgaata tcttcgcacc
     5581 atttcgccaa aggcgaataa cgttgtgaaa tacactccgg caaaatagtt cttatactgc
     5641 acggctccgg tcaatcaggc cggacgccgg cgtcagcaag caaaccaagc acaatttgtc
     5701 tccagttgcc tcgtggtttc agcgctcgaa gatttccaaa atgaccgagc agcgttctat
     5761 gcacaaagat caagtcgggt ggaaacacaa tgtttcttga ttcaagaaag tctgaagtac
     5821 ccatttgcca tagttttgaa tagatatccg gtatggatcc aaagacagtc tcctcgtccg
     5881 gaaatacttg atttagcatt cttgcgattg gctggataaa ttcttcggta agtttttctc
     5941 cgctcttata gcgaatcccc gctgcaaaca aaagaccggg tacgggcgaa agtctgccgc
     6001 tcaagagttc cgcggcggcc tttgagtagc tgtctgaaag agattccggc accagtttga
     6061 cacagccaaa gtcataaagc acaatgcgcc cgtcggaaag aaatcctacg ttagccgcgt
     6121 ttggatccgc gtgcaagaat ttatgttcta ccgccccctt cacgatcgca gtaaggagat
     6181 gtacggccca ctggaatcga agagtctccg cagacacgga cgcttctgca atactcatgg
     6241 acggaataaa gttcgtggtg agaacgcggc ctgtgcagaa ttctcttact ggcgtcggga
     6301 tcacaattgg atctgaatac atctctgttg cctgatccag gtgagaggct tcgcgtacgt
     6361 agtcgatctc ctcaagaagc ctatccttca cctcaccaat cagctttcgc gcaggcacgg
     6421 cagtcatcgg ggaaaaagca gtcatgatcg tccgaagtat tcttagatca cttcccagag
     6481 cttcgcgtat tcccgggtat tgcactttga ttgcgaacac cctaccatct ggatgcgaag
     6541 cgcgatgtac ctggccaaga gacgccgcgg cgattggctt ttcttccagc ctgagttgcg
     6601 atgccttttc ttcgcccagc tccagcctca gtatatcttg aatttcagaa acagcaattg
     6661 gttctgcatc cttccgcagt ccggccagca gtccggagat ttctggaggc agcagtcctt
     6721 catgcagaga aagcatttgc ccgagtttca tcggcgcccc tttcagtttg ctcaggcttt
     6781 cgatggcgcg cgccgccgat ttcaaacgcc gttgctcggt cggatcgcca agcaatgccc
     6841 acccgagtcg accggcggca gtcgtgagtt tccagaatcg gtcaaaaccg aaatcaatca
     6901 ctgagcgctt ccgcgaaagc tgcgatgatt tcttcgattg gctctattcc aacagaaaca
     6961 cgaaccaggt ccggatccag gtgtagactg gcaagctcgc gtcttcccgc ctccgactgg
     7021 accagatcgt aatgggcaag atacatgaac gggcaaagaa tcgtaaaaac agaaccaaaa
     7081 ctcgggcctt tagcaactgt tatgcgatca tagaatttct cgagcggaat attgagttca
     7141 atcgtaatga tcccgcccgg cgcctccgct ctgcgctgaa acttctcata gttcttgcgc
     7201 gactgttctg cataggccca gtatactttc ttaatgtttt tgtgatttga gaggtatgtt
     7261 gccagtcgca tcgtatttgc attgattttc gcgactacag agtgatagtt ctcgatctgt
     7321 tctgcgaggc gcgcaagatc gcgcgggtag ggattctcaa tggatgtttc tactttgcgc
     7381 gaaagctctt tgtagaacgg agatgcaggg tttagaacag cagagccgat catcacgtct
     7441 cccatatgag aggcatattt cgtaaggctg ttgatgacgg cgtcagaatg caccagaact
     7501 tcaacgttca gaggcgtgac cagagttgga tccaggatca gcgccgcgcc tgcggacctg
     7561 gtaattgcgt gcaattcctg gagatctgtc atctcaacga gcgggttggt cgggcattct
     7621 gtgacgatcc ctgcgatcct gtctgagttt tttgaaacaa aatcgcgtat cgcgcccatg
     7681 tcgaaaacgt cgcccatgaa tgcgcgatct gacggagcag tgaatttctt gatgatttct
     7741 cccgtatcta cgtagagcca tccgagctgc aaccagagag tgcggccctt tgtggcctga
     7801 atttcctgaa gaccgcggaa cactgtgaag aacgcgttca ttccgctcgt tgcaagatgg
     7861 acgtgggact gcgaagtctt gtgcgcctcc cccagaatct aaaggacacg cttgctatcg
     7921 gtctctgccg cctgc
//
LOCUS       DAORFF010000109         7929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4512_length_7929_cov_4.047879, whole genome shotgun sequence.
ACCESSION   DAORFF010000109 DAORFF010000000
VERSION     DAORFF010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4512_length_7929_cov_4.047879"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PKK76_06090"
     CDS             <1..901
                     /locus_tag="PKK76_06090"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNL68074.1"
                     /translation="SWFDAEGRFTLATSDGFRGQYAGTTAGISASLLAEEDGVKQTDS
                     WYSYDRFVSRLDTPEAVGKEAARRTVARLGSRKVKSQTAALVVDPLVSRRFVSMIFGA
                     ASGGSIYRKSSFLLDRLGTVIAAPGVTIDDDATLEDGPASRPFDGEGLPGRRLRLVEN
                     GTLGEIPCESYFARKLGRSPTGNAVRGYASGPAAGSTNLFMQPGSDSPSDIIRSVKNG
                     LYLNSLMGMGFNGVTGDLSQGASGYWIENGEIAFPVQEITIAGNFLDMLAGIERIGND
                     LSFKMGSTAAPTLLIREITIGGS"
     gene            complement(903..1592)
                     /locus_tag="PKK76_06095"
     CDS             complement(903..1592)
                     /locus_tag="PKK76_06095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNL68075.1"
                     /translation="MEAKLPSRPGMFAVIDTTQGSLTIELFPDAAPKTVKNFADLAQK
                     GFYKGIVFHRVIPDFMAQTGDPTGTGSGGPGYQFEDEINPKALGLDKIKVKDAPEYGR
                     QAQQLAVKNLDITSQSQLNAKRAQFEAEVRKIGEMTVAEVLEKSGYKYQDSLPSRKAI
                     RGAVAMANAGPNTNGSQFFINQVETPHLNGLHTVFGQLEASDFEVLDKIIAAGNGNTK
                     IKDVQVFDKRK"
     gene            complement(1691..2677)
                     /locus_tag="PKK76_06100"
     CDS             complement(1691..2677)
                     /locus_tag="PKK76_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68076.1"
                     /translation="MRTLRQWLRLITAHPAESLGIAVALGQLAFLRSLPILWLLILWI
                     PAALIAYLILRLTIPRILRRFNSIHNHWIELIVSALTLTILYLFLGLPWWSAVLLICV
                     FAFMVLAIADKRPGAFYVIYYGLLIFILFAGAHGILLAEFRTVAWLMDKHETGAMHEE
                     VKDGKWTILRGNNPILEFAILPEMKVSHDVNMEWIPGNALGVLSPGGGISPPGLIPFE
                     MTHKETGEKISFLLGGMNLSGTAESLTPPRRDSLSFSGVELQGLVFGFKNPQSGRLMQ
                     IALYCSSKFDLSFLVIEEVQPGFPHSPLILQQLDQARPGLQTGNPNCVFDNP"
     gene            2771..3094
                     /locus_tag="PKK76_06105"
     CDS             2771..3094
                     /locus_tag="PKK76_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL68077.1"
                     /translation="MHAEIHKDGTTLILRAVYPRLDGGNHLLNALKSGPWLEGDVEAV
                     EIDFGDVEYLNSLGITELVTIHRLVQEQTRGKTRFRFFNVDRKIRTILELVEVQKIAE
                     IVPRP"
     gene            3180..3800
                     /locus_tag="PKK76_06110"
     CDS             3180..3800
                     /locus_tag="PKK76_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68078.1"
                     /translation="MFGFDVAGPVNARTRENLETLARTREAWARFFPKREGIRIQMHA
                     GMMLPGAYDSSADIAYLNPQFDQDSFRHELAHALIWRNAGDVPYWYQEGAAYFLQSAG
                     EARCGGTVMLSKKMAERALGRIQSKQDALPWDAVDLGDRSSALDTAALSAAFFLFAWS
                     KRDLEGLLKTPPQMKGPPPWLNDHADFEKSFRAFLETGLSKPVPGC"
     gene            complement(3805..5091)
                     /gene="rmuC"
                     /locus_tag="PKK76_06115"
     CDS             complement(3805..5091)
                     /gene="rmuC"
                     /locus_tag="PKK76_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNL68079.1"
                     /translation="MTELLIVFNVLATCGILLLVLLTFLRKADQHGPDREILVRLDAQ
                     DRLIEKLERTLREEAARSRQENSILAKTGREEIQTTLQKTTDSSGTRLTEMSRLQLQS
                     LDTMRKAVEERLRALQEENVKKLDQMRATVDEKLQSTLERRLSESFRVVSDRLDQVHK
                     GLGEMQNLAIGVGDLKKVLTNVKTRGTWGEIQLSLLLEQILSPDQYQKNVRTRSGGTE
                     FVEFAIKIPIRDEVMWLPVDSKFPQELFERLGDAQEKADVELAEKASRELEAFIKKSA
                     KEIAEKYIEPPHTTDYAILFLPTEGLYAEVIRRPGLASSIQSDYRIAIAGPTTLAALL
                     NAFQMGFRTLAIQKRSSEAWKLLEQVQHEFSKFGTLLDKTQKKLQEATDSIESARGRA
                     DKIQKRLGQAGRLRLDEGGSEIEMNGDESQELLGLK"
     gene            5168..5983
                     /locus_tag="PKK76_06120"
     CDS             5168..5983
                     /locus_tag="PKK76_06120"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNL68080.1"
                     /translation="MSILRMWRDPRHYQLGALYSLFLYGAFALGFDVKPLHVVLTIAT
                     AVTTQVLFSWIFRVPVELRSALITSCSLCLLLRSSVWWVPVFAAFVAMGSKFVLRWNG
                     KHIFNPANGAIILCLLVFDNAWVSPGQWGNTAFFGFLFACLGGLVVMRAKRADVSLAF
                     LFFYSAMIFARSLYLGEPMTIPIHRLESGALLLFTFFMISDPKTTPNSRVARILYAFL
                     VAAGAYYVTFKMFRTNGLLWSLVIVSLLVPFMDLLFRGEIYQWRKKDEVQFSS"
     gene            5961..7271
                     /locus_tag="PKK76_06125"
     CDS             5961..7271
                     /locus_tag="PKK76_06125"
                     /inference="COORDINATES: protein motif:HMM:NF021597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HNL68081.1"
                     /translation="MKFSSAVKYSTVALAIVTSVEVVYAFCGFYVAKADTKLFNQASS
                     VVLVRDGDRTVLTMSNDFQGDPKEFALVVPVPTFLERGQINVGDPAVIAHVDAFTSPR
                     LVEYFDSDPCMVEYERDDVRRAEVPSANTQGPGRHNDLGVKVEAKYTVGEYDILILSA
                     KESGGLETWLKQNGYKIPPGASEVLGSYIKQNMRFFVAKVNLKEKAKTGFTYLRPLQI
                     AFESPKFMLPIRLGTVNANGPQELFVFAITKQGRIETTNYRTVRLPTDINVPEYVKSE
                     FADFYKSMFAEMVRKEDMRAVFLEHAWDMSWCDPCAADPLSPAELKKLGVFWVKESEG
                     GAVPAFVTRLHVRYDRAHFPEDLVFQQTGDKANFQARYIMQHPFKGATSCPAGERYKS
                     QLVVRQNQEAENLAKLTGWDIEAIRKKIGIMKATERKWWQNLWD"
     gene            7284..>7929
                     /locus_tag="PKK76_06130"
     CDS             7284..>7929
                     /locus_tag="PKK76_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HNL68082.1"
                     /translation="MRTLYIECVSGIAGDMFASAFLDAGLISRQEVEDLPRRIGWPDV
                     KIEVSSVVRATMKATSIDVSAQGESWKQSLKESASAPIPSGKIEIRGKAHEATHSLSA
                     RPVHSDGGAGSPLHNHMHGDSHGHTHGHSHGGTHANADGWHTQYADLDRFLATRDLPI
                     GVIEKARSIFYNLGRAEAEAHGMAIEYVAFHEVGAYDSIIDVLLAAICIDRIGVD"
BASE COUNT         1832 a         1902 c         2100 g         2095 t
ORIGIN      
        1 atcatggttc gatgcggaag gcagattcac tctggctact tccgacggtt ttcgcggtca
       61 atatgccgga accactgctg gaatttctgc ctcacttctg gcagaagaag acggcgtgaa
      121 acaaacggac tcctggtatt cgtatgatcg ctttgtttcg agactggata ctccggaggc
      181 ggttggaaaa gaagcggcca ggagaactgt ggcaaggctg ggatcgagga aagtgaaatc
      241 acagaccgca gctcttgtag tggatcctct ggtttcgcgt agatttgtaa gcatgatttt
      301 cggagctgcg agcggcggaa gtatctatcg caaatcttcc ttcctgttgg atcgactggg
      361 cacggtgatt gccgcgccag gagtcacgat agacgacgat gcaactctgg aggatggccc
      421 ggcttcgcgt ccctttgatg gggaaggact tccgggacgg agactcagac tcgtggagaa
      481 cggaacgtta ggcgaaattc cgtgcgagtc ctatttcgcg cgcaagcttg gtcgcagtcc
      541 aaccggaaac gcagtccgcg ggtatgcttc cggtccggcg gctggaagca ccaatctatt
      601 catgcagcca gggtcagaca gtccatcgga tatcattcgt tcggttaaga acggtcttta
      661 cttgaattct cttatgggaa tgggttttaa cggagtaacg ggagatctat cgcaaggagc
      721 ttctggatac tggattgaaa atggagaaat tgcgtttcca gttcaagaga tcacgatagc
      781 cggcaatttt ctcgatatgc ttgccggcat cgagcgaatt ggaaatgatc tgagctttaa
      841 aatgggatcc acggcggctc ccactttgtt gatcagagaa attacaatcg gcggtagttg
      901 acctattttc ttttatcaaa gacctgcacg tctttgattt tcgtatttcc gtttcctgcg
      961 gcgatgatct tgtctagaac ttcaaaatca gaagcctcta gctgaccaaa cactgtgtgc
     1021 agaccgttga ggtgcggtgt ctctacctga ttgatgaaaa actgcgatcc atttgtgttt
     1081 ggacctgcat ttgccatggc aaccgcacct ctgattgctt tgcgcgatgg cagagaatcc
     1141 tgatatttgt agccgctttt ttctagaact tctgccactg tcatttctcc aatcttgcga
     1201 acttccgctt caaactgcgc tctttttgca ttcagttgcg attgtgaagt gatatccagg
     1261 ttttttacag cgagctgctg ggcctgtctg ccatattctg gtgcatcttt tactttgatt
     1321 ttgtcaaggc caagagcctt cgggttgatt tcatcttcga attgatatcc gggtccgccc
     1381 gatcctgttc cggtaggatc tcctgtttgt gccataaaat caggaatcac gcgatgaaac
     1441 acaatccctt tgtagaatcc tttttgcgcg aggtctgcga agttcttcac agtctttggc
     1501 gccgcatccg ggaacaattc aattgtaaga gaaccctgtg tcgtatcaat gactgcaaac
     1561 attccaggcc gcgatggcag cttagcttcc atgcgcgcac aaaataaaat tgagaagaag
     1621 gatgtcgcga tgagcgcgac agagcgaata tttttcaaca taatgaaaaa tcgattctgg
     1681 ttcgaggctg tcaaggattg tcgaacacgc aattcggatt cccggtttgt agaccgggac
     1741 gtgcctggtc cagctgctgg aggatcagag gagaatgcgg aaagccaggt tgcacttctt
     1801 caatcacaag aaatgaaaga tcgaacttgc tcgagcaata cagtgcgatt tgcatgagcc
     1861 ttccgctctg cgggttttta aatccaaaca cgagtccttg aagttccaca cctgaaaaac
     1921 tcagactgtc tcttcgcggc ggagtcaaag attcagcagt tcctgataga ttcattcctc
     1981 ccagaaggaa ggatatcttt tcccctgttt ctttgtgggt catttcaaac ggaatgagac
     2041 ctggaggcga aatgccacct cccggcgaga gcacgccaag tgcattgccc ggaatccact
     2101 ccatgttcac gtcgtgcgaa actttcattt ccggaagaat tgcaaattca agaatcgggt
     2161 tgtttccgcg caggatcgtc catttcccgt ctttgacttc ctcatgcatc gcacctgtct
     2221 catgcttatc catgagccat gcgaccgtgc gaaactccgc gagcaaaatc ccatgcgctc
     2281 ccgcgaatag aatgaagatg agcaggccat agtagatgac gtagaatgcg ccgggccttt
     2341 tgtctgcgat cgcaagaacc atgaaggcga agacgcatat caacagcacc gcgctccacc
     2401 agggtaggcc caggaaaaga tacagaatgg taagtgtaag ggcggaaaca atgagttcta
     2461 tccagtgatt gtggattgaa ttgaatcgcc ggaggatgcg cggaatggtg agccggagga
     2521 tgaggtaggc aatcaatgct gccggaatcc agagaatcaa gagccagagg atgggcaggc
     2581 ttcttagaaa cgcgagttgc cccagagcaa cggcgattcc caggctttca gccggatgag
     2641 ctgtgatcag ccggagccat tgtcgcaaag tgcgcatcta atcccgaaaa acaatctggt
     2701 tcctccttgc atcttaaaat ttgcatcagt tttgatttgt ggaaaacgac aggctgcgat
     2761 ttctggcatt gtgcacgctg aaatccacaa agacgggacc accttaatcc tgcgcgccgt
     2821 gtatccgcgt ctggacgggg gcaaccatct gctcaatgca ctcaagtctg gcccgtggct
     2881 ggaaggggac gttgaggcag tggagattga ttttggcgat gtagaatacc tcaattcgct
     2941 cgggattaca gaacttgtga ccatccacag actggtgcag gagcagacgc gcgggaaaac
     3001 aaggtttcgt ttcttcaatg tggaccgcaa gattcgtacg attcttgagc ttgtggaagt
     3061 tcaaaagatt gctgaaatcg ttccgagacc ttaaattcag cctttttccc ttaataattg
     3121 cagttgtagg agctcctaca tcttgtgcga gctcccagat ctggcaaaga gaacagactt
     3181 tgttcgggtt tgatgtggcc gggccagtca atgcgcgaac aagggagaac ctcgaaacac
     3241 tcgcccggac gcgggaagcc tgggccaggt tttttccaaa aagagagggg atccggatcc
     3301 agatgcacgc tggaatgatg cttcccggcg cgtacgattc atccgcagac attgcctact
     3361 tgaacccgca gtttgatcaa gacagctttc gccatgaact ggcgcatgct ttgatctggc
     3421 gcaacgcagg tgacgttccc tactggtacc aggaaggagc agcgtacttt ttgcagtctg
     3481 cgggagaagc cagatgcggc ggaactgtga tgctttccaa aaagatggca gagcgcgcgc
     3541 ttggtaggat ccaatcgaaa caagacgctc tcccctggga tgcagtcgat ctgggagatc
     3601 gctccagtgc cctggacaca gcggcattgt ccgctgcatt cttcttgttt gcatggagca
     3661 agcgcgatct ggaaggactc ctgaagacgc cgccgcagat gaagggtcca ccaccctggc
     3721 tcaatgatca cgccgacttc gaaaaatctt ttcgcgcgtt tcttgaaacg ggactctcca
     3781 agcctgtccc gggttgctga tgaactattt caagccgagt agttcctggg attcatctcc
     3841 gttcatttct atttcggatc caccttcgtc caggcgaagt ctgcctgcct gacccagacg
     3901 tttttgaatt ttatcagcgc gccccctggc gctctctatg ctatccgttg cttcttgaag
     3961 tttcttttgc gttttgtcga gtaacgtccc gaatttgcta aattcatgct ggacctgttc
     4021 cagtagcttc cacgcttcgc tggacctttt ttgaatcgcg agcgtgcgaa agcccatttg
     4081 aaaagcattc agaagggcgg ccagtgtagt gggtcctgca atggcgatgc ggtagtccga
     4141 ctggatgctc gaagcgagcc cgggccttcg aatgacttct gcgtacagac cttctgttgg
     4201 aaggaacagg atcgcataat cggtagtatg cggaggttca atgtatttct ctgcgatttc
     4261 cttcgcggat tttttgatga acgcttccag ttctctcgat gctttttctg caagttcgac
     4321 gtcagctttt tcctgcgcgt cacccagcct ttcaaacaat tcttgaggaa atttggagtc
     4381 caccggaagc cacatgactt catcgcgaat aggaatctta attgcgaatt ctacaaattc
     4441 tgttccaccg cttctggttc gcacattctt ctggtactga tccggtgata agatttgttc
     4501 caataaaagt gatagctgaa tttctcccca ggtgccccgt gttttcacat tcgtgagaac
     4561 tttcttgagg tctcccactc caatcgccag attttgcatt tctccgaggc ctttgtgtac
     4621 ctgatcgagt ctgtcgctga ccactcggaa tgattcactg agccgtcttt cgagagtgga
     4681 ctgaagcttc tcgtccacag tggcacgcat ttgatctagc ttctttacgt tttcttcctg
     4741 gagcgcgcgc aatcgctctt ccactgcttt gcgcattgta tcgagactct gcagttgcaa
     4801 ccgcgacatt tctgtaagtc gggttccact tgagtctgtg gttttctgga gagtggtctg
     4861 gatttcttcg cgaccagttt ttgcgaggat ggaattctcc tggcggctgc gcgccgcttc
     4921 ctcgcgaaga gttcgctcca gcttttctat gaggcgatcc tgagcgtcga ggcgaacgag
     4981 gatttcgcgg tccggtccgt gctgatctgc tttgcgaaga aacgtgagta gaacgagcag
     5041 tagaattccg caagtggcca gaacgttaaa aacgatgaga agttcggtca tggtcagcat
     5101 gataccgtgt cactgtgtca gaaatgatcg tttttcatat tttttttgaa cgagaacgta
     5161 gttttttttg tccattcttc gtatgtggag agatccaaga cactatcaac tgggcgcgct
     5221 ttattctttg tttctatatg gtgcctttgc gctggggttt gatgttaagc cgttgcatgt
     5281 ggttttgacg atcgcaactg cggtgactac tcaggtgctt ttcagttgga tttttcgagt
     5341 ccctgtggag ctgcgaagtg ctctgattac atcttgttct ctctgtcttt tgcttcgctc
     5401 cagtgtctgg tgggttccag tgtttgccgc ctttgtggcg atgggaagca agttcgtgct
     5461 ccggtggaac ggaaaacaca tcttcaatcc agcgaatggt gcaatcattc tgtgcttgct
     5521 cgtgtttgac aacgcgtggg tttcaccggg ccaatggggc aacacagctt tcttcggatt
     5581 cctgtttgca tgtcttggcg ggctcgttgt catgcgtgcg aaacgtgcgg acgtaagtct
     5641 tgcattccta ttcttttact ccgcgatgat tttcgcaagg tctctgtatc tgggtgagcc
     5701 aatgacgatt ccgatccaca gactggaaag cggcgccctt cttttgttta cattctttat
     5761 gatttcagat ccgaaaacaa cgcccaattc tcgcgttgcc agaattctat acgcgtttct
     5821 ggttgccgcg ggagcatact acgtgacttt taagatgttt cgcacgaacg gtcttctctg
     5881 gtcgcttgtg atcgtttcgc ttctggtgcc attcatggat ttgctatttc gcggtgagat
     5941 ttaccaatgg agaaagaaag atgaagttca gttcagcagt taagtattcc accgtcgccc
     6001 ttgcgattgt tacttctgtg gaagttgtgt acgcgttttg cggcttctat gtggcgaaag
     6061 cggacacgaa gttgttcaat caggcgtcga gcgtggtgct ggttcgcgac ggagatagaa
     6121 cggttcttac tatgtccaat gactttcagg gagacccgaa ggaatttgca cttgtggttc
     6181 cagttcctac gtttcttgag agaggccaga tcaatgtagg cgatcccgcc gtcatcgctc
     6241 atgtagatgc gttcacttcc ccaaggctgg tggagtattt tgattctgat ccatgcatgg
     6301 ttgaatatga acgcgatgac gtgcgaagag cagaagtgcc gtccgcaaat acgcaaggac
     6361 ctggaagaca caacgacctg ggtgtaaagg tcgaggctaa gtataccgtg ggagaatacg
     6421 atattctgat tctatccgcg aaggaaagcg gcggtctcga aacctggctg aagcagaacg
     6481 gatataaaat tccacccggc gcctcagagg tcctgggaag ctacatcaag caaaatatga
     6541 ggttctttgt tgcgaaggtg aatttgaagg agaaagcaaa gaccggattt acataccttc
     6601 gtccgcttca gattgcattc gaatcgccta aattcatgct cccgatccga ctgggcaccg
     6661 tgaatgcaaa tgggccacag gaattgttcg ttttcgcaat cacaaagcag gggcgcattg
     6721 aaaccacgaa ctatcgcaca gtccgacttc ctactgatat caatgtgccg gaatatgtaa
     6781 aaagcgaatt tgccgatttc tataagtcca tgtttgctga aatggttcgc aaagaagaca
     6841 tgcgcgcggt ttttcttgag catgcgtggg atatgagctg gtgcgatcca tgtgccgccg
     6901 atcctctctc tcccgcagag ctgaaaaaac ttggagtatt ctgggtgaag gaatccgagg
     6961 gtggggctgt tcctgccttt gtgactcggc tgcatgtccg ctacgaccgt gcgcattttc
     7021 cggaggacct ggtattccaa caaacgggtg ataaggcgaa ctttcaagct cgctatatta
     7081 tgcagcatcc gtttaaaggt gccacctctt gcccggcagg agagcgttac aaatcacagc
     7141 tcgtcgttcg tcagaatcag gaagctgaga accttgcaaa gctcaccggt tgggatatag
     7201 aagcgattcg caagaagatc ggtatcatga aggcgacaga gagaaaatgg tggcagaatc
     7261 tctgggatta atcatcccgg agcatgcgca ctttatacat tgaatgcgtc tccggaattg
     7321 ccggagacat gttcgcgtct gcgtttctgg acgctggatt gatttcgcga caagaagtgg
     7381 aggatcttcc tcgaaggatc ggttggccag atgtaaagat agaggtcagt tcagtggtgc
     7441 gcgccaccat gaaagctaca tcgatcgatg tatcggcgca gggcgaatcg tggaagcaat
     7501 cgctcaaaga atccgcgagc gcccccatac cctcgggcaa gatagaaatt cgcggcaaag
     7561 ctcacgaggc aacacattcg ctttcagccc gcccggttca ttcagatggg ggggcgggtt
     7621 ctccactgca caatcatatg cacggagatt cgcatggaca tactcatggc cattcgcacg
     7681 ggggcactca cgcaaatgca gacggatggc atacgcaata tgcggatctg gatcgattcc
     7741 tggccacgcg cgacctaccg ataggcgtca ttgagaaggc tcgtagcatc ttttataacc
     7801 ttgggcgggc ggaagcggaa gctcatggga tggcaatcga atacgtagca ttccacgaag
     7861 tgggcgctta cgacagcatc atcgatgtgc ttctggcagc catttgtata gaccgtattg
     7921 gagtggatc
//
LOCUS       DAORFF010000110         7902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4530_length_7902_cov_2.913980, whole genome shotgun sequence.
ACCESSION   DAORFF010000110 DAORFF010000000
VERSION     DAORFF010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4530_length_7902_cov_2.913980"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="PKK76_06135"
     CDS             complement(<1..1571)
                     /locus_tag="PKK76_06135"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL68083.1"
                     /translation="MFRAFDLRSQAIVLLCLLTVSPALSGESAAKLYQDGMQALSRGD
                     RENARYYFRRALEVNPGYREAALADGDLNLQMGDLKRARESYDRMLSVDAGNVRALVG
                     LARVNTLTGQYSEADQLLARAEKADPSNESAFHARGDLNWRLGRRELAAGSFERALRK
                     NPSHLPSLLSLAVLETERGRTERASSLIARARKIDPENPEVSLAEGDIAFRRLLSEKD
                     PVERKRLVSIARRSYQNSLQIAPGSVPVQSNLVLLDLYSGTSKDTASRIRSLMDQEPQ
                     NPRWYYLHAILAPGKIQDTLYASGRALSLAPQDSLIRYRHESLLIANPDVAGLEARRN
                     AARFHGRLMSAAGSAGRTDLRDFHAEASLRLYSDSREALTILVDQARRQGDYEGYLDL
                     LLRMRQLDPSPSIQFQIDEALRQKNKDMAMRDGLYSRSSGSFRQNFERSPRRILVFDF
                     KSETPFPRIPDAGEILASALRDHLSVKTAPMQEYKRKKLFDSVRAAEGRPGPVFFNPA
                     IVRNIPEDSVSAIVAG"
     gene            complement(1555..3591)
                     /locus_tag="PKK76_06140"
     CDS             complement(1555..3591)
                     /locus_tag="PKK76_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNL68084.1"
                     /translation="MSHRAAILSALLLLLSPMLAQRLPSFRIKEEESRDHFRRGLTFY
                     NSGQYLAAREFFYKALNRVPSFHLARRYLGDANYYSGEWNSALEQWEFLDKRSEGTYS
                     LIRMRSDLLRFRLNGFRDAGPYIHFRTFNKKDFGDISALRPVDIAVDSEANVYYLFSE
                     SANIIVSNAGATSFRQIRGSIFDRMKGPSAMALSKDRIYVADYSDDRIRIFDRSGSSI
                     SSFGTTGSEKGQLRGPAGIAVTADAVYVSDSGNRRIEKFDLDGKHILSFLTADTGLKF
                     PAGIAVDGENVYVADRDSARIFTFDRDGNQLDQVESRMLSHPRGVSVGAGKLVIADEE
                     AGVLFYDLAEKTWTRLETRTEDDRTLVWKRAYSARMDSNGVLMAADTGAGLVHMFMPE
                     ALRISNLECRVERVDTASFPDVAVFLQLRNRLGNPLPGLDRNEIRIFENDRFLRGINT
                     SSMEDYQNRVNLSFVKENNADLSSEENKNVLSTILTSFLAPIRISDRIKVIRAGESVR
                     SVYEGLQRRMIIRSMQEGETTERPDVGKALVEAIGGQITSLGPRAVIAIVSGASANPF
                     VQYPVDRVEYFARAQGIPIYIISFEEGGPGDTQDVYRSLATNTGGAYIRAYDETAVRA
                     LYESIRARKDERYIITYHSGLTPDLADRYVDVRIDVDHQGMTGSAEGGYFVPRL"
     gene            complement(3695..4534)
                     /locus_tag="PKK76_06145"
     CDS             complement(3695..4534)
                     /locus_tag="PKK76_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68085.1"
                     /translation="MKSQKIWHVSEGRKFPLDLKKHDTLSFQIMEISPNDLRTLHPEP
                     DDLHVFLIQGERGGLAAMRNLMESTPHLSDFPRIIILPQDQYGLYEANPRNMNLLVLD
                     DSVRPGHLKLILETVLRVEHYRQIVFHMSEQMREQAGLLDKMLGLARDEIKNARSESA
                     AFEALMDFETVHKRFERSIADAMERTMRLKDQELLEMKSTLAAYEHLSEFRDRELSET
                     RKQMHATESVLDLSHRENMEREKIIQALERLRMLTDKELIDLFEENQTLRSRLGMEPR
                     LFP"
     gene            complement(4549..6399)
                     /locus_tag="PKK76_06150"
     CDS             complement(4549..6399)
                     /locus_tag="PKK76_06150"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNL68086.1"
                     /translation="MMTARIAQWIPSFLKPRILDRYIFLEILAPFLVWLALLTTLFMS
                     IVLKDIAGELFGKGISPFKISVYIFYLIAEKITQTIPVACLFSGILAAGRLSGDSEIV
                     AMRSAGISFPRIYVIFIFFGFLATLLVAFMNLYYGPVSARAREDFENWIKSYHSLSLV
                     KTGRFLGGAKMDGVSLKGQDIYAGRRDGEELKEVHIREWLNGLNELSSERIMIRNISI
                     PIGDGFITQIVHAKSGQLVERVKIKNAESSQEPDVPLDLPPGVDPEQFKKAKKTPVPE
                     EESKTEAVLRLKDGFVIEIAPDLSRVQTTDFTNGSMDYVIPPAPKPLGRLNVKPDNYT
                     LWELYDFLDKLEAGGTEIDPMAILGADVAAAKIGEKAAGSNFTFKLPGLSAMKVMAVQ
                     NQMWIAMNAANRGKEGGPTEEQFQAMMTLTVQVEVFLKDAEKTSRKFKVEIQNRFAKS
                     VACMLFFFISFPLGLVVKRSGRGMSFSLALVVFVAYFGLYLYGTGLGESGKAHPAIGA
                     WLPDVVVAFIGVYVMASRTEGFKQRFRALVSNAKRKWDTIVWSRLSPVLMPVFTPVWK
                     AIRWVGSLVARILGPVARPVAGVVRRSVNGLRSSIFAPLAKRLLAFRNRS"
     gene            6383..7429
                     /gene="waaF"
                     /locus_tag="PKK76_06155"
     CDS             6383..7429
                     /gene="waaF"
                     /locus_tag="PKK76_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNL68087.1"
                     /translation="MRAVIIQTAFLGDAILTTPMFAAFKRFFPDSYLAAVVKPDAAQV
                     LKGLPYLDEIISFDKKKSHRGISGLRKLAADLQSRDFDILLSPHSSYRTAFLSRLSKI
                     PERYGYLDASFSRIAYNHRLPRHSSQPEILRLLSFLSDSVCPDASKASTDLVLAEDQA
                     CVLEAESVLRKHEASRPILLAPSSVWATKRWTPAGFAELAGKLVRKHKCKILLVGSPS
                     DKAICEQVMAFIRDLQPLFVQDRVTVAAGETSLRGFYSLVKRSRLLVSNDSAPVHYAC
                     AAKTPVVALFGPTVPALGYAPITKNSRIAEINLACRPCGTHGGKVCPLSHFRCMKELT
                     ADMVMAKVSELLPE"
     gene            complement(7722..>7902)
                     /locus_tag="PKK76_06160"
     CDS             complement(7722..>7902)
                     /locus_tag="PKK76_06160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNL68088.1"
                     /translation="GYETLYGHLRSISVKKGQEVRASQEIGKSGSSGASTGPHLHYEI
                     RFAGKTVHPGDFLVF"
BASE COUNT         1891 a         2111 c         1931 g         1969 t
ORIGIN      
        1 cccgctacaa tggcagatac agaatcttcc ggaatattgc ggacaatggc tggattaaaa
       61 aatacagggc caggtcttcc ttctgccgct ctcacagaat caaatagttt ctttctttta
      121 tattcttgca tgggtgcggt ctttacagac agatgatcgc ggagcgcgga ggcaagaatc
      181 tctcccgcat ccggaattcg cgggaatggt gtttctgatt tgaaatcaaa cactagaatt
      241 cttctcggag atctttcgaa gttctgccga aacgagccac tcgaacgcga atacaatcca
      301 tcgcgcatcg ccatgtcttt gttcttctgt ctgagtgctt catcgatctg aaactgaatc
      361 gacggagacg ggtcgagctg gcgcatgcga agaagtaaat ccagatatcc ttcgtagtcg
      421 ccctgtctcc tggcctgatc cacaaggatt gtgagcgctt ctctgctgtc tgaataaaga
      481 cgaagggatg cttccgcgtg aaagtcacgc aaatctgttc ttcctgctga acccgcagca
      541 gacatgagtc tgccgtgaaa tcgcgctgca tttcttctgg cttcgaggcc ggcgacgtct
      601 ggattcgcaa tcagaagaga ttcgtgcctg taacgaatca gagaatcctg cggtgcaaga
      661 gaaagcgcgc ggccgctcgc atacagggtg tcctgaattt tcccgggagc gaggatcgca
      721 tggaggtaat accagcgcgg attctgtggt tcctgatcca tgaggctgcg gatgcgcgat
      781 gctgtgtctt tagatgttcc tgagtagaga tcgagcagca ccagattgct ttgaacgggc
      841 acactcccgg gcgcaatttg aagagaattc tggtacgatc tgcgggcaat ggaaacgagc
      901 ctttttctct ctacggggtc tttttcagat agaaggcggc gaaacgcaat gtctccttct
      961 gcgagggaca cttctggatt ctccggatct atttttctag cacgtgcaat gagcgaagaa
     1021 gctctttctg ttctgcctcg ttccgtttca agaacggcga gagatagtaa actgggaaga
     1081 tgagacggat ttttacgaag agctctctca aaacttccgg cagcaagctc tcgcctgccc
     1141 agacgccagt tcagatcgcc gcgcgcatga aatgcggatt cattggaagg gtccgctttt
     1201 tccgcgcgag caagcagttg atctgcctcc gagtactggc ctgtaagagt attcacccgt
     1261 gccagtccga caagggctcg aacatttccc gcgtccaccg atagcatccg atcataactt
     1321 tcgcgcgctc ttttcaaatc tcccatctgc agattgagat ctccatctgc gagagccgct
     1381 tctctatagc ctggattcac ctcgagggcg cgtctgaaat aataccgtgc gttttctcgg
     1441 tcgcctcgcg aaagagcctg catgccgtcc tggtacagtt tggccgcact ctcacccgaa
     1501 agcgcaggcg acacagtcaa aaggcagagc aaaacaatgg cctgcgatcg cagatcaaag
     1561 gcgcggaaca aagtaacctc cctccgcaga cccggtcatg ccctggtgat ctacatcaat
     1621 tcgcacatct acataccgat cagcaagatc gggggtcaaa ccagaatgat atgtaatgat
     1681 atacctttcg tcttttcgcg cacgtatgct ttcatagaga gctcgcacag cagtttcatc
     1741 gtaagcgcga atgtaagcac cacctgtgtt tgtcgcaagc gaacggtaga cgtcctgtgt
     1801 atcccctggc ccaccctctt caaaagaaat aatgtagatg ggaatgccct gcgcgcgcgc
     1861 aaaatactct actctgtcca cgggatactg cacaaatggg ttggcgcttg cgcccgaaac
     1921 aatagcaatc acagcgcgag gaccaagaga agtgatctgt ccaccaatgg cttccacgag
     1981 cgccttgccg acgtctggcc tttctgtcgt ttccccttcc tgcatgctac gaatgatcat
     2041 gcgtcgctgc agaccttcat acacggatcg cacgctttct cctgcgcgaa tgactttgat
     2101 tcggtctgaa atgcgaatcg gtgcgagaaa tgacgtcaaa atcgtgctca gaacattttt
     2161 gttctcttcg ctggatagat ctgcgttgtt ttctttcaca aaggatagat tcacgcgatt
     2221 ctgatagtcc tccatgctgg aggtattgat gccgcgtaga aaacgatcat tctcaaaaat
     2281 gcgaatctca tttcgatcca gacccggaag tggattcccg agcctgtttc tcagctgtaa
     2341 aaacactgcc acgtcgggga aagaagcagt gtccactctc tccaccctgc actccagatt
     2401 gctgatgcgg agcgcttcag gcatgaacat gtgaaccagg cccgctcctg tatctgcggc
     2461 catgagaact ccatttgaat ccatgcgcgc agaatacgct ctcttccaca caagagtgcg
     2521 atcgtcctct gttctggttt ccagtctggt ccacgtcttt tccgcaagat catagaagag
     2581 tacacctgct tcctcatctg cgatcacaag ttttcctgcc ccgacggaca cgcctcttgg
     2641 atgagagagc attcttgatt cgacctgatc gagctgattg ccatcgcgat caaacgtgaa
     2701 aatgcgagca gaatctctat cagccacgta aacgttttcg ccatccaccg caatgccggc
     2761 gggaaactta agccctgtat ctgcagtcaa aaacgatagt atatgttttc catctaaatc
     2821 aaatttttca attctacgat ttccactgtc cgaaacatac accgcgtccg ctgtcactgc
     2881 aattccagct ggtcctcgca gctgcccctt ttcgcttcca gtggtaccaa atgaagaaat
     2941 gctcgatcct gatcgatcaa agatgcgaat gcgatcatca gaatagtctg caacgtaaat
     3001 gcgatctttt gaaagtgcca tcgcagacgg tcctttcata cgatcaaaaa tcgaaccgcg
     3061 aatctgacga aacgaagtgg ctcccgcatt cgatacgatg atgttggcag attcgctaaa
     3121 cagatagtag acgttcgctt cgctgtcgac ggctatatcc acgggcctca gcgcggagat
     3181 gtctccaaaa tcttttttat tgaatgtgcg aaagtgaatg tatgggccag catcgcggaa
     3241 tccattcagg cgaaagcgca ggagatcgct tcgcatacga atcaaagaat aagtaccttc
     3301 tgatcgctta tccagaaact cccattgttc cagagcgcta ttccattcac ccgagtaata
     3361 gtttgcatct ccgagatagc gccgcgcgag atgaaacgac ggaacacgat tgagagcttt
     3421 gtaaaaaaat tctctggcag ccaggtactg accggaattg taaaatgtaa ggccacggcg
     3481 aaaatgatcg cgcgattctt cttccttgat gcgaaaggaa ggcagcctct gtgcgagcat
     3541 cggcgacagg aggagcaaca gtgcgctcag gatcgctgcc ctatgtgaca taatccagcg
     3601 tgaagtctgg aatcgcttcc ttcaagcctt ttttaggcct gttttctccc ttcccagcgg
     3661 gactggcctc cgacccggag tgacggcgaa ggcctcaggg gaagagtcga ggctccattc
     3721 ccagcctcga acgcagggtc tggttctcct caaatagatc gatgagttct ttgtcagtga
     3781 gcatcctgag cctttcgagt gcctggatga tcttctccct ttccatgttt tccctgtggc
     3841 tcagatccag gacagactcg gttgcatgca tttgtttacg ggtttcggaa agttcgcggt
     3901 cgcgaaactc gctcaaatgc tcatacgcag ccagagtgct cttcatttcg agcagctcct
     3961 ggtccttgag tcgcatggtg cgttccatgg cgtccgcgat ggatctctcg aaacgcttgt
     4021 ggaccgtctc aaaatccatc aaagcctcaa atgcggcgga ctctgagcgg gcgtttttga
     4081 tttcatcacg cgcaaggccg agcatcttgt cgagaagccc cgcctgctct ctcatttgtt
     4141 cggacatatg aaatacgatc tgacgatagt gctctactcg aagcactgtt tcgaggatca
     4201 atttcagatg acctggacga accgaatcgt ccaggacaag caggttcata ttccgtggat
     4261 tggcctcata gagtccatat tgatcctgcg gtagaatgat gatacgcggg aaatcggaaa
     4321 ggtgtggagt cgattccata agatttcgca tggctgccag gccgccccgt tcgccctgga
     4381 tgaggaatac gtgaagatcg tctggctccg ggtgcagagt tctcaggtcg ttggggctga
     4441 tctccatgat ctgaaagctc agagtgtcgt gcttcttgag atccagggga aactttctgc
     4501 cttccgatac gtgccagatt ttctgcgact tcatagcctg tgtacagttc aggagcggtt
     4561 gcggaaggca agcaaacgct tggccagagg agcaaatatt gatgagcgaa gaccgttcac
     4621 acttcgcctt acgaccccgg cgaccggacg agccaccggg ccgagaattc gggccacgag
     4681 actgccgacc catcggattg ccttccagac cggggtgaac actggcatca agacaggaga
     4741 cagacgcgac catacaatcg tgtcccactt tctttttgca ttggacacaa gtgcgcggaa
     4801 gcgttgcttg aatccttccg ttcgagaggc cattacgtat acgccaatga acgctaccac
     4861 tacatcggga agccatgctc cgatggcagg atgagctttg cccgattctc caagccctgt
     4921 tccgtacaga tacaaaccaa agtacgcaac aaacacaacg agtgccagag agaagctcat
     4981 ccctcttcca gatcttttta caaccaggcc cagtggaaat gaaatgaaaa agaacagcat
     5041 gcacgcaacg gatttcgcaa aacgattctg aatctctact ttgaacttcc ggcttgtttt
     5101 ttctgcgtcc ttgaggaata cttctacctg cactgtaaga gtcatcatcg cctggaattg
     5161 ttcttctgtt ggcccgccct cttttccccg attggcagcg ttcattgcaa tccacatctg
     5221 gttttgcacc gccatcactt tcatcgcaga taggccgggc agtttgaaag tgaagttcga
     5281 tccagcggcc ttctctccga tttttgccgc ggccacatcc gctcccagaa tcgccattgg
     5341 atcaatttca gttccacctg cctcaagctt gtccaggaaa tcgtacagtt cccatagagt
     5401 gtagttgtcc ggcttcacat tcaatcgacc caggggttta ggtgcaggtg gaatgacgta
     5461 atccatgctg ccatttgtaa aatcagtagt ttgaacgcgc gaaagatcgg gagcaatttc
     5521 aatgacgaat ccatctttca aacgaagcac tgcttctgtt ttgctttctt cttctggcac
     5581 cggagtcttt tttgctttct tgaattgctc gggatccact ccaggcggca gatccagcgg
     5641 aacgtctggt tcctgggatg actctgcatt cttgatcttc actctttcca caagctggcc
     5701 actcttcgca tgaacgatct gagtgatgaa accatctccg attggaatgg agatgttgcg
     5761 aatcatgatt cgctcactgg atagttcatt gagtccgttg agccattcgc gaatgtgcac
     5821 ttccttgagc tcttctccgt ctcgccttcc cgcgtagatg tcctggcctt tgaggcttac
     5881 gccgtccatc ttcgccccgc ccaggaacct gcctgttttc acgagggaaa ggctgtggta
     5941 ggacttgatc cagttttcaa agtcttcgcg cgcccgcgcg ctgacgggcc catagtagag
     6001 attcatgaac gcgactagca acgtggccag aaagccaaag aagatgaaga ttacatagat
     6061 ccgaggaaaa ctgattccag ccgatcgcat ggctacgatt tcactgtctc cggaaagcct
     6121 gcctgcggcg aggattcctg aaaacagaca ggcaactgga atggtctggg tgattttctc
     6181 agcgatcaga tagaagatat agactgagat tttgaaaggg gaaatccctt tgccaaacag
     6241 ttcccccgca atgtctttga ggacaatgga cataaaaagc gtggtcagga gggccaacca
     6301 gacgaggaat ggtgcgagga tttccagaaa aatgtagcgg tccagaattc tgggcttcaa
     6361 aaacgacgga atccattgcg caatgcgagc agtcatcatt cagaccgcct ttctcggcga
     6421 tgcaatactt accacgccta tgtttgcggc cttcaagcgt ttttttcccg attcgtatct
     6481 ggccgctgta gtaaaaccag atgcagccca ggtcctcaaa ggccttccct atctagacga
     6541 aatcatatcc tttgataaga agaaatcaca ccgcgggatt tctggccttc gaaaactcgc
     6601 cgcagacttg caatcgcgtg attttgatat tctactttct cctcattcat cttatcgcac
     6661 tgcatttctt tcgcgtcttt ctaaaattcc agagagatac gggtatctgg acgcgagctt
     6721 ttcgcgcatc gcctacaacc atcgcctgcc aaggcattct tctcagccag aaattctacg
     6781 attgctttcc tttctttcgg attcagtctg tccggacgcg tctaaagcat ctacggacct
     6841 ggtgcttgca gaagatcaag catgcgtgct ggaagcggag tctgtgctgc gaaagcacga
     6901 agcatccaga cccattttgc tcgcaccttc ttctgtctgg gcgaccaagc gctggactcc
     6961 ggccggattc gcagaactgg cagggaagct ggttcgaaaa cacaaatgca agattctact
     7021 cgttggatct ccttcagaca aagcgatctg tgaacaggtg atggcattca ttcgagatct
     7081 tcagccactc tttgttcaag atagagtcac tgtggcggcc ggtgaaacat cgctgcgcgg
     7141 cttttattca cttgtaaaaa gatcgcgcct ccttgtatca aacgattctg cgccagttca
     7201 ctatgcctgt gccgcgaaga ctcctgtggt cgccttgttc ggaccaacgg tgccggccct
     7261 gggctacgca ccgattacaa agaattcgcg aatcgcagaa atcaatctgg catgcagacc
     7321 atgcggcacg catggaggaa aagtctgtcc cctttcccat tttcgctgca tgaaagaact
     7381 cacggcggac atggtgatgg caaaagtttc agagcttcta ccggaataag atccgcagcg
     7441 gacacctgcg cgccaggaat cctgcacgtg gtcaatacgc ggcgcaccca ttcgcaatac
     7501 cagagaatga tacgtattcc atacgatctg gttttccgct cgttcccggc gcttttttca
     7561 cgacccgacg cattcgccgc gttttatacg cactcgatgt aatacgaaca aagaatgcgc
     7621 gatattgcgc acgcccgtat gtaatacgtc attttattgc gtgataaaac cgcgggatgg
     7681 cgtgcgggaa cgacaaggcg ctgcaggccc ccggagcgat tttaaaagac cagaaagtct
     7741 cctggatgaa ctgtctttcc tgcgaatcga atctcatagt gaagatgcgg acctgtggac
     7801 gctcctgagg atccggattt accaatctcc tgactggcac gcacttcctg ccctttcttc
     7861 acagaaatgg atctcagatg accgtataac gtttcgtaac cg
//
LOCUS       DAORFF010000111         7870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4557_length_7870_cov_2.791811, whole genome shotgun sequence.
ACCESSION   DAORFF010000111 DAORFF010000000
VERSION     DAORFF010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4557_length_7870_cov_2.791811"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="PKK76_06165"
     CDS             complement(<1..1340)
                     /locus_tag="PKK76_06165"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNL68089.1"
                     /translation="MHGFKYAWTIRSPPEFTVCKSARILILARTSAMLFNSGIFILLF
                     FAVYSFYWLLPVRAKQYMIIITSILFYGWYSVPFLIMFLALLIINYYVGMSLIDKKNK
                     KVLIFGIVVDVMVLVIFKYFYLLAETVGHITGSAYLIDLRNSWVADYKFEIILPIGIS
                     FYTFQVIAFITDCYTGMIREKVTPMKFYVFTLFFPHFVAGPIMRAPDLIPQIDNPSID
                     RTRMLNACLLILMGIVKKVLIADRLGAATSAIWHNPEKYDAIFLLLIPFVWAFQIYGD
                     FSGYTDMARGIAKLLGFHIPENFRGPFVARSYQELWQRWHMTLSFWLRDYIYIPLGGS
                     RVTPFRTYMNLMITFAVGGLWHGAAYNTIVWGLYTGLVLSLERMWGFSRFRLLPEGRF
                     NFLRILYTYVVFSIGCLIFASPSMHHTFAIIKGVLSWQRGIPGGAIETLIGLSVL"
     gene            1335..>2635
                     /locus_tag="PKK76_06170"
     CDS             1335..>2635
                     /locus_tag="PKK76_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNL68090.1"
                     /translation="MQELSEKPGLPAWDLKAIYPDSSSRDADVALLRNLVVKFVEEYR
                     GRLGSLDDDGLVRAFEAHAKISNTSSRIMVHAYLTFAENTASTAAQALLASAEELSAE
                     VWGQLVFFELELGRLPGQFSSRMDAYAYFVQKCVQRNAHNLTEELEAYGIEKNLTGVQ
                     AMTGLFDEVMGSLSFEDKNASGEDTTFTQETALSVLHHADRAYRLRIYSRFLETVGSQ
                     KTVLTSIYNNVLLDHRLDWKRRNYTGAMEPRNLSNQVSSRAVDSMLEIVQSSYRIVRR
                     YYTWKARYAKIDQFTNADIYAPVLPRTSPYDFAHAQDSVIRAYAGFDPQAGSIVSDFF
                     KTARVDARVIPGKRPGAFCYGASPELDAFVHLNYTGDLRSIQTLAHELGHGLHHQLSR
                     SQNHVNFGTPLVTAETASVFGEILLNELLIQEARSKEEQLAL"
     assembly_gap    2636..2735
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2736..3274
                     /locus_tag="PKK76_06175"
     CDS             <2736..3274
                     /locus_tag="PKK76_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNL68091.1"
                     /translation="LNELLIQEARSKEEQLALLCAQMEGILATVFRQTVLTRFEERAH
                     AERDSGRVSADRFSELWLEENGKLYGDAVSMVDAYKWGWAYIPHFVHTPFYCYAYSFG
                     QLLVLALYQEFRTHGDSFKDGYMKLLSLGGSRAPAKLVSETVGLNIESDDFWLRAVSF
                     IENMMDRIESIGPGPGVA"
     gene            3278..3808
                     /locus_tag="PKK76_06180"
     CDS             3278..3808
                     /locus_tag="PKK76_06180"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNL68092.1"
                     /translation="MSAKTPRSSSVETRHLVMPDHANHYGTLFGGTLMGWIDMVAVMV
                     AQRHCGREAVTASVDRLNFLSPISVGDHVLLRASVNYTGRTSMEIGVQVSRENPYTGE
                     VVRATTAYLTFVALDENKKPVPIPPVEPETEIEKRRFRNAILRVEANRNLVKKMREEM
                     QSSPLPDSSGASPQEK"
     gene            3924..4334
                     /locus_tag="PKK76_06185"
     CDS             3924..4334
                     /locus_tag="PKK76_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNL68093.1"
                     /translation="MCKREVAELLRSHDINPTTQRIEIAHLIFQKAQHLCADEILAEL
                     NKEYEQVSQATVYNTLKVFVDKKVVRELVFAPDRIYYDSNTSHHHHFIDVDTGKIYDV
                     SGDCVKCPELSYVDHLKAEVLEVSLILKGRMPQA"
     gene            4393..5718
                     /locus_tag="PKK76_06190"
     CDS             4393..5718
                     /locus_tag="PKK76_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNL68094.1"
                     /translation="MDDALLIARLQFAFTITFHYLFPQLTMGLALLILVQKAMFMFTR
                     QLSYNHAAQFWARIFAVNFAMGVVTGIPMEFQFGTNWARFSVFAGGVIGHVLAMEGVF
                     AFFIESAFLGLFLFGERRLGQRAHTFTAFMVFLGSWMSGYLIIITNAWMQHPVGFEAA
                     SGKLELVRFADLFFNPWAGWQYLHTMFGAVVTASFVMAATGAYYLLSNRDIRYGEIFL
                     RVGVTCGLAASVLMIFPTGDGQGKNVAQYQPVTLAAMEGLFETGKAAEMILIGQPDME
                     RLRIDNPIRIPGVLSLLTYSRWNAEVKGLQEFPRELWPTNIPLLYYSYHIMVGLGTYM
                     AAVMALSALFLFRRHLFDHKWLLWILMLSFPAPYIANTAGWMTEELGRQPWLIYGILK
                     TTAGVSPTVSAGNSLFTLMGFLGMYGLLGTLFLFVIAREIQNGPEHGHH"
     gene            5720..6763
                     /gene="cydB"
                     /locus_tag="PKK76_06195"
     CDS             5720..6763
                     /gene="cydB"
                     /locus_tag="PKK76_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNL68095.1"
                     /translation="MQIETFWFIALVFTLTMYVVLDGFDLGVGILHLTVAKTDDERRT
                     LFNAIGPLWDGNEVWLIAAGGVLFFAFPVAYASSFSGFYLPLMMILWLLMFRALAIEF
                     RRHIENELWQQFWDVAFCVSSLGLVLLLGMALGNVLRGVVFGEDHFFFAPLWTDFTPT
                     GEPGIIDWFTLSFGLLAVSALSGHGAAFVVMRTEGDLRDRAAGIYRKMFISTLALSAL
                     CLGAASWIHPEYLDRFTHFPPSMLLPAGSLGCFVASRFFLLRSDRLPFVFSCLMIVLA
                     GSASAAALFPKLLPGLSSTGGLDAFNASSAAYGLQIGQYWWLFGMALVSVAFAYLYFV
                     FRGKVGSQKRKHY"
     gene            6839..>7870
                     /locus_tag="PKK76_06200"
     CDS             6839..>7870
                     /locus_tag="PKK76_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNL68096.1"
                     /translation="MDQNLDTLRSAFGEIKDLYDHAPCGYHSLDPTGLIIKINETELN
                     WLGYSREEIEGRVRLWDLVTPQSKDTCTTYFADLQKYGSVKEVEIELFRKNGSVIPLL
                     INAVALYDENGKFIQTRATVFDITERKRAEKKLEESEGRLLAFISNIPAVIFVKDTTG
                     RYLFVNEEFQRVFGLEGGQILFRTDAEIFSAEQAESFKKNDELVIKTGKRIEFEETAI
                     YTDGPRTSIVHKFPIRDDSGEIVALGGIATDITERQRISRALERVNRALRVLSGANEV
                     IVHAETEESLYSSVCHVIVTRGSYRMAWIGLAQHDEEKLVRPVGHQGGDMDYLTSIRV
                     TWDDSEHGMG"
BASE COUNT         1866 a         1863 c         2010 g         2031 t
ORIGIN      
        1 agcacagaaa ggccgatcag tgtttcaata gctcctcctg gaattccacg ctgccacgac
       61 agtacacctt tgatgattgc gaaggtatga tgcatactcg gcgatgcgaa gatcagacag
      121 ccaatggaaa atactacgta agtatatagt attcgcagaa agttgaatct accttccggt
      181 aggagtctga atctggaaaa tccccacatg cgctcgagtg acaggacaag gccggtgtac
      241 aatccccata cgatcgtatt gtaggctgct ccgtgccaca gtccacccac agcaaacgtg
      301 atcatgagat tcatatacgt gcgaaaggga gtaactcgtg atccgcccag cggaatgtag
      361 atataatcgc gcagccagaa agaaagagtc atgtgccatc tctgccagag ctcctgataa
      421 cttctggcaa cgaatggtcc gcgaaagttc tccgggatgt gaaatccgag gagctttgca
      481 attcctcgcg ccatgtccgt atatccggag aaatccccgt aaatctggaa ggcccagacg
      541 aacggaatga gcagcaggaa gatcgcatcg tatttttcag gattgtgcca gatcgcagaa
      601 gtggcggcgc ccagtctgtc tgcgatgagc acttttttta caatgcccat caggatcagg
      661 aggcaggcgt tcagcattct ggttctgtca atgctagggt tgtctatttg cggaatgaga
      721 tccggcgctc tcataatggg cccggctaca aagtgcggga agaagagagt gaacacgtag
      781 aacttcatgg gagttacttt ttcccggatc attcctgtgt aacaatctgt gatgaaggcg
      841 atcacctgaa acgtgtagaa gctaatcccg attggaagga tgatttcaaa cttatagtcc
      901 gcgacccagc tgtttcgcag atcaatcaga taggcgctgc ctgtgatgtg cccaaccgtt
      961 tcagccaaaa ggtaaaaata cttaaatatt accaggacca tcacatcgac tacgatcccg
     1021 aatatcagaa cttttttgtt cttcttgtct atgaggctca tcccaacata gtagttgata
     1081 atgaggagtg ccaggaacat gatcaggaat ggcacactgt accagccgta aaacagaatg
     1141 ctcgtgatga tgatcatgta ctgcttcgca cgcactggca ggagccagta aaacgaatac
     1201 actgcgaaga acagcaggat gaagattcct gagttaaaaa gcatggcgct ggttcgagca
     1261 aggatcagga tacgcgctga tttgcaaact gtaaattctg gtggactgcg gatagtccag
     1321 gcatacttga agccgtgcaa gaactctctg aaaagcccgg cctccctgcc tgggatctaa
     1381 aagcaatcta tccggattca tcttctcgcg acgcggatgt cgcactgttg cgcaacctcg
     1441 ttgtcaagtt cgttgaagaa taccgcggcc gcctgggctc tctggacgat gatggtctgg
     1501 ttcgggcttt tgaagctcat gcaaagatct cgaacacatc ttcgcgcatt atggtgcatg
     1561 catatctgac cttcgcggag aacacggcaa gcactgccgc gcaagctctt ctggcaagtg
     1621 cggaggaact cagtgctgaa gtctggggcc agcttgtgtt cttcgagctc gagctggggc
     1681 gattgccggg ccagttctct tctcgcatgg atgcgtacgc gtactttgtt cagaaatgcg
     1741 tacagagaaa tgcgcataac cttacggagg aactcgaagc ctacggaatc gaaaagaatc
     1801 tgaccggagt tcaggcaatg accggcctct ttgatgaggt gatgggatct ctttctttcg
     1861 aagacaaaaa tgcgtcggga gaggatacaa ccttcaccca ggaaaccgcg ctctctgtgc
     1921 tccatcatgc ggatcgcgct tatcgccttc gcatctacag tagatttctc gagactgtag
     1981 gttcgcagaa gactgtacta accagtattt ataacaacgt cttactcgat cacaggctag
     2041 attggaagag gcggaactac accggtgcga tggagcctcg caatctatcc aaccaggtga
     2101 gttcgcgtgc agtcgattcg atgctcgaga ttgttcaatc cagctatcgc atcgtcagga
     2161 gatactacac ctggaaagca cgttatgcga agattgacca gtttaccaac gcggacatct
     2221 atgctcccgt tcttccgcga accagtccat acgattttgc tcatgcgcag gattcagtga
     2281 ttcgtgctta cgccggattc gatcctcagg caggttcgat cgtttctgat ttttttaaga
     2341 cagcgagagt ggatgcgcgc gtcattccag gcaaacgtcc aggtgctttt tgttacggcg
     2401 cttcccctga gctggatgca ttcgtgcatc taaactacac cggcgatctt cgctccattc
     2461 agactctagc gcatgaactg ggacatggat tgcatcacca gctgtcacgc agtcaaaacc
     2521 acgtgaattt cggaacccct cttgtgaccg ctgagactgc ctctgtgttc ggagagatct
     2581 tgctcaacga gcttctgatt caagaggcaa gatcaaagga agaacaactg gctctnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgctc aacgagcttc tgattcaaga
     2761 ggcaagatca aaggaagaac aactggctct actctgcgcg caaatggaag gaattctggc
     2821 cactgtattt cgccaaacag tgcttacgcg ttttgaagaa agagctcacg cagagagaga
     2881 ttcaggacgt gtttccgcgg atcgattcag cgaactgtgg ctggaagaaa atggaaagct
     2941 ctatggcgac gcagtatcca tggtggatgc gtacaaatgg ggatgggcgt atattccgca
     3001 ttttgttcac actccatttt actgttacgc ctattcattc gggcaactcc tggttctcgc
     3061 attgtatcag gagtttcgca cgcacggaga ttcgtttaag gacggttaca tgaagttgct
     3121 atcgcttggt ggatcaagag cgcctgcaaa acttgtaagc gaaacagtgg gtctgaacat
     3181 tgaatcagac gatttctggc ttcgcgctgt gagtttcatc gagaacatga tggacagaat
     3241 tgaatcgatt gggccgggcc cgggagtcgc gtgagccatg tctgctaaaa cgccgcgctc
     3301 ttcgtctgta gaaactcgtc atttggtaat gccagatcat gcaaatcact atggcacgtt
     3361 attcggcggc actctcatgg gatggattga tatggtcgcg gtgatggtgg cccagcgcca
     3421 ttgtgggcgc gaagccgtca ctgcgagtgt ggatcgtcta aattttcttt ctcccatttc
     3481 tgtgggcgat catgtccttc ttcgcgcaag cgtgaattac accggtcgca cttctatgga
     3541 gataggcgtt caggtttcgc gtgaaaatcc ttatacaggg gaagtggttc gcgcaaccac
     3601 cgcgtacttg acctttgttg ctctggacga aaacaagaaa ccagtgccca tccctcctgt
     3661 ggagcctgaa acagaaatag agaaaagacg atttcgaaac gccatcctgc gcgtagaagc
     3721 aaacagaaat ctggtaaaga aaatgaggga ggaaatgcaa tcctcaccgc ttccggattc
     3781 atcgggtgcc tctccacagg aaaaatgaga catagtttgc tttccggata ggccctgagg
     3841 cctaaaaaag gggttgtcat ccctaaatcc cctgtctaag ttgatagcgg ttactgccga
     3901 tggaagacca gaagaagtat ggcatgtgca agcgagaagt ggcggagctg cttcggtcgc
     3961 atgacatcaa tcctactacg cagcgcattg aaatcgcgca tttgattttc cagaaagccc
     4021 agcacctttg tgcagatgaa atcctcgcag aactcaacaa agaatacgag caagtcagcc
     4081 aggcaacagt atacaacact ctgaaagtat ttgtagataa gaaagtagtc cgcgagctag
     4141 tatttgcgcc ggacagaatc tactatgatt ccaacacaag ccatcatcac catttcatag
     4201 atgtagacac cggtaagatt tatgacgttt ccggagactg cgtcaaatgc cctgagctca
     4261 gctacgtaga tcacctcaaa gcagaggtgc ttgaagtgag tttgatcctc aagggtcgca
     4321 tgcctcaggc ataatcactc acgcatcgtc tttttgtttt gactctgaaa cacgcttcca
     4381 atagaaagcg gcatggatga cgcgctttta atcgcccgcc tgcagttcgc gttcaccatc
     4441 acgtttcatt atcttttccc ccagctgacc atgggccttg cacttttgat cctggtgcaa
     4501 aaggcaatgt tcatgttcac ccggcaactc tcctacaatc atgcagcgca attctgggct
     4561 cgcatctttg ctgtaaattt tgcgatgggc gtggtgaccg gaattccaat ggaattccag
     4621 ttcggaacaa actgggccag gttttctgtc ttcgcgggcg gagtgattgg ccacgttctt
     4681 gccatggagg gagtatttgc gtttttcata gaatctgcgt tcctgggtct ttttcttttt
     4741 ggagaaagac gccttgggca gcgtgctcac accttcacag cattcatggt attccttgga
     4801 tcctggatga gtggatactt gattatcatt acgaatgcct ggatgcaaca tcccgttggt
     4861 tttgaagctg cgtcaggtaa gcttgagctt gttcgatttg cggatttgtt tttcaacccc
     4921 tgggctggct ggcaatattt gcacactatg ttcggtgcag tggtcactgc atctttcgtg
     4981 atggcggcca cgggtgcgta ctaccttctc tcaaatcgcg acatcagata cggcgaaatc
     5041 tttctgagag ttggggtcac gtgcgggctg gctgcctccg tgcttatgat ctttcctacg
     5101 ggcgacgggc agggaaagaa tgtggcgcag tatcaacctg ttactctcgc cgcgatggaa
     5161 ggactctttg aaactgggaa agcggcagag atgattttaa tcggacagcc ggatatggaa
     5221 cgccttcgca tcgacaatcc aattcgcatt ccaggagtac tcagtctcct gacgtattcg
     5281 cgctggaacg cggaggtaaa agggctccag gaatttccgc gcgaactctg gccaacgaac
     5341 attcctcttc tctattatag ctatcacatc atggttggtc tgggcacata catggccgcg
     5401 gtgatggcgt tgagcgctct gtttttgttt cgcaggcatc tcttcgacca taagtggctt
     5461 ctgtggattt tgatgctttc ttttccggcg ccttacatcg cgaatacggc aggctggatg
     5521 acggaggaac tggggagaca accctggctc atttacggaa tcttgaaaac taccgctggc
     5581 gtttcgccta ctgtgtccgc gggaaatagc ctgtttactc tgatgggatt tcttggaatg
     5641 tacggactcc tgggtactct tttcttgttt gtgattgcgc gcgaaatcca aaacggcccg
     5701 gaacacggac atcattgata tgcagatcga aactttctgg ttcatcgctc ttgtgttcac
     5761 tctaaccatg tatgtggttc ttgatggctt tgatctgggc gtcggcattc ttcacctgac
     5821 ggtggcaaaa acagacgatg agcgtcgcac tctgttcaat gcgattggtc cactctggga
     5881 tggaaacgag gtctggttga tcgcggcagg cggagttttg ttttttgcct ttccagtggc
     5941 gtacgcgtct agcttcagcg gattttatct tccgctgatg atgattctgt ggcttctcat
     6001 gtttcgagct ctcgccattg agtttcgtcg tcatatagaa aacgaattgt ggcaacagtt
     6061 ctgggatgtc gcattctgtg tttccagcct gggtctggtt ctcttgctcg gaatggctct
     6121 cggaaatgtt cttcgcggtg ttgttttcgg agaggatcat ttcttctttg ccccgctctg
     6181 gacggacttc actcccaccg gtgaacccgg catcatagac tggtttacac tttcgtttgg
     6241 acttctggcg gtcagtgctc tctccggtca cggagctgcg ttcgttgtta tgcgaacgga
     6301 aggagatttg agagacaggg cggctggaat ttatcgaaaa atgttcatca gcacattggc
     6361 gctttccgcg ttgtgtctgg gtgcggcttc ctggatccat ccagaatatc tggatcggtt
     6421 tactcatttt ccgccttcca tgctccttcc cgcgggatct ctgggttgtt ttgtggccag
     6481 tcgctttttc ctcttgcgat cggatcgact tccatttgtt ttttcctgtt tgatgattgt
     6541 tctggctgga agtgcaagtg cggcggctct ctttccaaag cttttgccgg gtttgtcctc
     6601 tactggcggt ctcgacgcgt tcaatgcttc gtcggctgcc tacggacttc agatcggcca
     6661 gtactggtgg ctcttcggaa tggcacttgt atcagttgca tttgcgtatc tatactttgt
     6721 ttttcgcggc aaggtaggca gccagaagcg caagcattac tgatttcagg atttcctccc
     6781 ggttaaaact cgctggctat ttcgatgcac ccaggaaaac atttacccgg gatcgaattt
     6841 ggatcagaac cttgacactc tccggtctgc gtttggcgaa atcaaagatc tgtacgatca
     6901 cgcgccctgc ggttatcatt ccctggatcc aaccggactg attattaaaa tcaacgagac
     6961 ggagctcaac tggctcgggt attcgcgcga agaaattgaa ggtcgtgtaa ggctctggga
     7021 tctggtgact cctcaaagca aagacacatg caccacgtat tttgcggatc tacagaagta
     7081 cggctctgtc aaagaagtgg agattgagct ttttcgaaag aatggcagtg tcattcctct
     7141 cctgatcaac gctgtcgctc tctatgatga aaacggaaag ttcattcaga cccgcgcaac
     7201 tgtctttgac atcacggaaa gaaaaagagc tgaaaagaaa ctggaagaga gtgaagggcg
     7261 acttcttgca tttatttcaa acattccagc cgtcatcttc gtgaaagaca caacaggacg
     7321 ttacctcttt gtcaacgaag agtttcagcg cgtctttggt ctcgaaggcg ggcaaattct
     7381 ctttcgcaca gatgcggaaa tcttttcagc cgagcaggca gaatctttca aaaagaacga
     7441 tgaactggtc atcaaaaccg gaaagagaat tgagtttgag gaaacggcta tctatacaga
     7501 tgggccgcgc accagcattg tgcacaagtt tccaattcga gacgactctg gcgaaatagt
     7561 agcgttaggc ggaattgcaa ccgatatcac agagcgccag agaatcagtc gggctctcga
     7621 aagagtcaac cgcgcgctca gggttctttc gggagccaat gaggtgatcg ttcacgctga
     7681 gacagaagaa tctctctatt cgtctgtgtg tcatgtgatt gttactcgtg gatcttacag
     7741 aatggcctgg attggcctgg ctcagcatga cgaagagaag cttgtgcgcc ctgttggtca
     7801 tcagggtgga gatatggatt atctaacttc gattcgcgta acctgggacg attcggaaca
     7861 cggcatgggg
//
LOCUS       DAORFF010000112         7863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4570_length_7863_cov_3.952997, whole genome shotgun sequence.
ACCESSION   DAORFF010000112 DAORFF010000000
VERSION     DAORFF010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4570_length_7863_cov_3.952997"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PKK76_06205"
     CDS             <1..499
                     /locus_tag="PKK76_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68097.1"
                     /translation="GVRNILLTAMRANVNGKGPELGAEIRFLRESGLELRTLYGQSWQ
                     RGHAKPVGNTIAFRAEPASSLLDNLWIRSDGKGDSFLRVQRYAATLFSPRLGLGRAFF
                     SLRAEHSVIHVHRLVPIYTPITQLSLLQSGISSSLLPDLIFKYGKTSGIDDYKSYEVG
                     YEVML"
     gene            552..1592
                     /locus_tag="PKK76_06210"
     CDS             552..1592
                     /locus_tag="PKK76_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HNL68098.1"
                     /translation="MSDPILAVVPLGFPWQTLDPFLFCVHHKDAYPQGDDRFAPRASL
                     SGRQIGNDFSYKDGWSMYHGEIVPGFPQHPHRGFETITVARAGFIDHSDSLGAAARFG
                     KGDVQWMTAGAGVVHSEMFPLLNKDSDNPLELFQIWLNLPAKSKMAAPYFSMYWDHLV
                     PKLELKDSAGHSIRLAAYAGPLDGKAITPPPDSWAAAAEADVRILTIRLDAGAQFEIE
                     KAGPGVNRVLYFFDGKSVSISGTVVKEKHAIQLNAEMNATIVATDGPCELLMLQGRPI
                     GEPVAQYGPFVMNTQEEIQNAFADYRRTQFGGWPWPDDAPVHPRDAGKFARHADGRVE
                     KPLDAMAASADL"
     gene            1638..1796
                     /locus_tag="PKK76_06215"
     CDS             1638..1796
                     /locus_tag="PKK76_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68099.1"
                     /translation="MNGKTAKLLRKFGAKKGSNVKDLKRVWMSLPAKERFKKRQEMLA
                     ELSGTKKS"
     gene            1883..2683
                     /locus_tag="PKK76_06220"
     CDS             1883..2683
                     /locus_tag="PKK76_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68100.1"
                     /translation="MNDSNAVTRIASRYSGPPRTGNGGYVCGVCAGLAYSQLGEQKQD
                     SFTFEVRYKSPVPVDLDVSLLSKDNLCVLESIDGKTTYAEGQSSKPVSTVLPAVVTLE
                     QARYATLQSIPEKEHAFPDCYVCGPARKVGDGLRILPGPVAEHSGMAAAVWNVLPDAM
                     SSSGDLSVPILWAALDCPGYFAYHFSHGGAMQVAVLAKMKCTVIRRRTESDQLVVIGW
                     MKRKKGPLVEVSTALLDGSEILAIADGTWMQPASWNPENTGHKSPQYS"
     gene            complement(2671..3528)
                     /gene="mltG"
                     /locus_tag="PKK76_06225"
     CDS             complement(2671..3528)
                     /gene="mltG"
                     /locus_tag="PKK76_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNL68101.1"
                     /translation="MPGFRAKAFLYSFLLCLISVPVFANAGAHGGRKRPPEIIWKVRA
                     GEKIRDLCQRLEREGACLCTHFESTAKSADLSFIPRSEKTLRKFEGIFVPGEYRIEKI
                     RGQDSQTRSARILGSLLAAARTRYDHSDLEGNALHHRITIASIVEKESVKKENFEEVA
                     SVFFNRLRTGQTLGSCPTVEYALGYHRPFLLESDVRIQSPYNVYRNKGLPPTPIAFFS
                     EGAWNAALHPKETEYQFFVFDWTTGKLGFSKSYEEHKDRASRARANFIRRFGAARLHA
                     MDKTKYYEY"
     gene            complement(3509..4537)
                     /locus_tag="PKK76_06230"
     CDS             complement(3509..4537)
                     /locus_tag="PKK76_06230"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNL68102.1"
                     /translation="MQKSLSAVRAVIPPHCMRPHAGRALLYMASGIIFYSASVAFAWF
                     VWEKSIYWLYPISWFVGGTASTSLFVLAHDCGHYSILKSRRIMDVLGHVLLLPVCYPF
                     YAWKHSHDAHHKYTNLLKREDSIYFDNAWIPETIEEYETEKSQRPFYAFLYRIARRFP
                     PLGAWMHHGHYLLSSPELYREDHRKKAVFSIWFVLIGMLTFGPAISFLSGSWFGAFHF
                     WFLPALCFQFWMAIYTFLHHTAEDVPFLPEGEWTPYRAQVECTVNVLWPRPISFLHGN
                     IDVHLPHHVAANIPAYWLRDAYAAIKNSEFASEVREVRFSPSYYLRQIRQCRLWDTKA
                     KAYAGFPS"
     gene            complement(4537..5610)
                     /locus_tag="PKK76_06235"
     CDS             complement(4537..5610)
                     /locus_tag="PKK76_06235"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNL68103.1"
                     /translation="MQPPVVPSISQIRASIPNHCFQPSVWKSLFYFFFDISTIAGLAS
                     LALYLDSWYFYPVYWFLQGTMFWALFVVGHDCGHGSFSHIKWLNNLIGHLSHTPILVP
                     YHGWRISHRTHHQHTGDVDKDETWYPVTETQYSHMTLSIRILRYYVFLFLFPLYLFRR
                     SPGRDGSHFLPGSDLFRPSEKWDVLTSTLLWGIFFAGLCVVGWKFGIVFLAKFYIAPY
                     FVFVVWLDLVTFLHHTAPDVPWYRGEAWNFVRGNLSSVDRSYGIFEWFHHNIGTHMVH
                     HLFIGIPHYKLREATSHMAPFLGDLYRKSDESIFRAFWRAFRSCHIVSDEGPVVFYRP
                     AATLGQESDSGKPSASLKEAGAR"
     gene            complement(5610..6149)
                     /locus_tag="PKK76_06240"
     CDS             complement(5610..6149)
                     /locus_tag="PKK76_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68104.1"
                     /translation="MIESTASEAVRVTNMIKTFVQDGIPFTIELQKQGEGFYIGISEE
                     GKPDIKLPVETAQILNGDTFSIQTAAGETKGRFYFAGDEVYVHIAGQTFRMKSVSPAD
                     LETNTSNLHRSPMPGKVIAVQVAVGDSVQKDQTIVIVEAMKMENAIKATLSGKVKAVH
                     CKVGDLVSPDLNLVELEGS"
     gene            6158..6946
                     /gene="mazG"
                     /locus_tag="PKK76_06245"
     CDS             6158..6946
                     /gene="mazG"
                     /locus_tag="PKK76_06245"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNL68105.1"
                     /translation="MRGCKPAVTGSVDNSLSEQWARLQDITRTLREPGGCNWDRAQDA
                     ESMRVYLLEEAYEVIEAVNRNDPALIREELGDLLFIVFFYSRLFEEKGVFSVQDVMRD
                     VSDKLVRRHPHVFGEQKDHSVQEVLQNWEKIKAKEKSSEREAFLPALLRAFKVQEKAA
                     RKGFDWENARDVLPHLESEVAEFRQAVADGESSARREEEIGDILFTIVNCLRHLGIHP
                     ETALHRSTDKFQDRFARMTESAGEGFMDLSLEEKELLWQKSKQK"
     gene            6949..>7863
                     /locus_tag="PKK76_06250"
     CDS             6949..>7863
                     /locus_tag="PKK76_06250"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNL68106.1"
                     /translation="MGPYTTIHLGGPADFFLEANSDQEIIQGLAFAEDKNLRVRILGG
                     GSNVIFSDAGFRGLVIRVATRGIELERETNHGSELRVQAGHEWDAFVEHCVRANLSGV
                     ECLSGIPGTAGGVPIQNVGAYGQEAKETIVSVRVYDRNAKSIISIPGTECEFGYRTSR
                     FKHREAGRFIILDVLFALRNDSSPPRYPELITKMQDPAISGISPHPLAVRTAVLSLRK
                     AKSMVHSAEDPDSISCGSFFTNPVLDAESLAKLEAKMKSSGLTMPRAYPDGHNFKVSA
                     AFLVENAGFHKGLVRGGAGVSTKHALALI"
BASE COUNT         1991 a         1876 c         2104 g         1892 t
ORIGIN      
        1 cggagttcgt aacattttgc tgactgccat gcgtgcgaac gtgaatggca aaggcccgga
       61 gctgggcgca gagattcgct tcttgcgcga gagcggtctt gagctgcgca ctctgtacgg
      121 tcaatcctgg cagcgcggcc atgcgaagcc tgtcggaaac acaattgcat tcagagcgga
      181 gccagcctcg agcctgctcg acaacctgtg gatcagatcg gatggaaaag gggattcatt
      241 tcttcgcgtc cagagatatg cggccaccct gttctctccc aggctgggat tgggccgtgc
      301 attcttctct ttgcgcgcgg aacattctgt cattcacgtg cacaggcttg tgccgatcta
      361 cactccgatc acccagctga gtcttttgca aagcggcatt agttcgtctc ttttgcctga
      421 tctgattttt aagtatggca agacgtcggg catagacgac tacaagagtt atgaggttgg
      481 atacgaagta atgctttaga cgttgctctt tttttgatcg cccaggccag acacgctctt
      541 tatatagaag aatgagcgat ccaatcctcg ctgtagtccc actcggtttc ccgtggcaaa
      601 cactggatcc gtttttattc tgcgtgcatc acaaagatgc ttatccgcag ggagatgatc
      661 gatttgctcc tcgcgcttcc ttgagtggca gacagattgg gaatgatttt tcctacaaag
      721 acggatggag catgtatcac ggggaaatcg ttccaggatt tccacagcac ccgcacagag
      781 gttttgaaac gatcacagtg gcgcgcgcag ggttcataga tcacagtgac tcactgggtg
      841 ctgccgccag gttcgggaaa ggagatgtgc agtggatgac ggcaggtgcc ggagtggttc
      901 attccgaaat gtttccactt ctgaataaag attcagacaa tccactggag ctttttcaaa
      961 tctggttgaa tcttcctgcg aaaagcaaaa tggccgctcc ctatttttcc atgtactggg
     1021 atcatctggt tccaaagctt gaattaaaag attcggcggg acacagcatt cgcctcgccg
     1081 cttatgctgg tccgctcgat ggaaaagcaa tcaccccacc acctgattcc tgggctgcag
     1141 ccgcagaagc ggacgttcgg attttgacga ttcgcctcga cgcgggtgcg caattcgaaa
     1201 tagaaaaggc cggacctgga gtgaatcgcg tgctgtattt ttttgacggc aaaagcgtga
     1261 gtatttcggg cacagttgta aaagagaaac atgcgattca actgaatgca gagatgaacg
     1321 cgacgattgt ggccacagac gggccgtgcg aattgctgat gctccagggt agaccgatcg
     1381 gtgagcccgt ggcgcagtat ggaccttttg tgatgaacac tcaggaagaa attcaaaacg
     1441 cattcgccga ttacagaagg actcagtttg gcggatggcc ctggcctgac gatgcgccgg
     1501 ttcatccgag agatgcgggt aagtttgcgc gtcacgcgga cggcagggtg gaaaagccgc
     1561 tcgatgctat ggccgcctct gcggacctgt gaatcgcttt tagttgacgc acgtccccac
     1621 ttgcggacac ttggcccatg aacgggaaaa cagcgaaact tctccgcaaa ttcggtgcaa
     1681 agaagggctc gaatgtaaag gatttgaagc gcgtttggat gtcccttcca gcaaaagaac
     1741 gcttcaagaa gcgtcaggaa atgctcgcag agctttccgg cacaaagaaa tcctgatcgc
     1801 tttcatcatc tcgaaattcc ggcgcgaatt tcaatatttt tcaatgtgac ataatccagg
     1861 ccagggccca ttcgggggga acatgaacga ttcgaatgcg gtgactagaa ttgcatccag
     1921 gtattctggg cctcctcgca caggcaatgg aggttacgtt tgcggcgtat gcgcgggcct
     1981 tgcttactca cagttgggtg aacaaaaaca ggattccttt acgtttgaag tacgctataa
     2041 gtctcctgtt ccagtcgatc tagatgtgtc tctgttatca aaagacaacc tgtgtgtcct
     2101 cgaatcgatt gatggcaaga caacctatgc ggaaggacaa tcttcgaagc ctgtttctac
     2161 tgttttacct gccgtggtta cactggagca ggcccgttat gcgacattgc aatccattcc
     2221 agaaaaagag catgcatttc cggattgtta cgtctgcggc ccggcgcgaa aagtggggga
     2281 cggcttgagg atactcccgg gacctgtggc agaacattca ggaatggcag ccgcagtctg
     2341 gaacgttctt ccggatgcaa tgtcttcgag cggagaccta tctgttccga ttctgtgggc
     2401 ggctcttgat tgtccgggct actttgcgta tcatttctct cacggcggcg cgatgcaagt
     2461 agctgttctt gctaaaatga aatgcactgt gattcgacgg cgaacagagt cagaccaact
     2521 cgttgtgatt ggctggatga aaagaaagaa agggccgttg gttgaagtgt ccacagcgct
     2581 tctggatggg tctgaaatcc tggcgattgc agatggtacg tggatgcaac ccgcatcgtg
     2641 gaatccagaa aacacaggac acaaaagtcc tcaatattcg tagtattttg ttttgtccat
     2701 cgcatgcaat ctggccgcgc caaatcgccg gatgaagttc gctcgggctc gactggcgcg
     2761 atctttgtgt tcctcgtagc tctttgaaaa gccaagttta cctgtggtcc agtcaaagac
     2821 gaagaactga tattctgttt ctttcggatg aagggccgcg ttccacgcgc cttcagagaa
     2881 gaaggcgatc ggtgtaggcg ggagtccttt gttcctgtag acgttatacg gcgactggat
     2941 gcgaacatcg ctctccagca aaaacgggcg atggtaacca agggcgtact ctactgtagg
     3001 gcaggatcca agagtctggc ccgtacgcag tcggttgaag aacacagacg ccacttcttc
     3061 gaaattttct tttttgacag attctttttc cacaatgctt gcgattgtga ttctgtgatg
     3121 cagtgcattt ccttcgaggt cggagtgatc atatcttgtt cgcgcggctg ccaacagaga
     3181 acccagaatc ctcgcagatc gcgtctgcga atcctggccg cgaatttttt cgattctata
     3241 ttctccagga acaaagatcc cttcgaattt gcgaagagtc ttttcggatc gaggaatgaa
     3301 tgaaaggtcc gcgctttttg ctgtgctttc aaaatgagtg caaaggcagg caccttctcg
     3361 ttctaacctt tggcagaggt ctctgatttt ttctccagcc cgcactttcc agatgatctc
     3421 cggcggacgc ttgcgcccgc catgtgctcc cgcatttgcg aatacgggca cggaaatcag
     3481 gcagagtaag aaagagtaga gaaaggcttt agctcggaaa cccggcatac gcttttgcct
     3541 tcgtgtccca cagcctgcac tgtctgatct gacgcaggta gtaagagggt gagaatcgca
     3601 cttctctgac ttcagatgca aattcagaat tcttgatcgc agcgtaagcg tcgcgcagcc
     3661 agtatgccgg gatgtttgct gcgacatgat ggggcagatg aacgtcaatg tttccgtgca
     3721 agaaagagat gggtctgggc cacagtacat tgacggtgca ttccacctgc gctctgtaag
     3781 gtgtccattc tccctccggg aggaaaggca cgtcctctgc ggtgtggtga aggaaagtgt
     3841 aaatggccat ccagaattga aaacaaagcg ctggcaaaaa ccagaaatgg aacgcgccaa
     3901 accaggagcc tgaaagaaag gaaattgccg ggccaaatgt cagcatcccg atcagaacaa
     3961 accatatgga aaaaaccgcc tttttcctgt ggtcttcacg atagagctca gggctgctca
     4021 atagatagtg accgtgatgc atccaggctc ccagcggcgg gaatcgtctg gcgatgcgat
     4081 acaaaaatgc atagaatggt cgctggctct tctctgtttc gtactcttcg attgtttccg
     4141 gaatccaggc gttgtcaaag tagatggagt cttctctctt cagcagattc gtatacttgt
     4201 gatgcgcatc gtgcgaatgc ttccacgcat agaatggata acaaacaggc aacagcagaa
     4261 cgtggccaag cacatccatg atgcgcctgc tcttgagaat agaatagtgg ccgcagtcat
     4321 gcgcgagcac aaacaatgaa gtggaggcag ttccgccaac gaaccagctg attggataaa
     4381 gccaatatat ggatttctcc catacaaacc atgcgaaggc gacggatgca gaataaaaga
     4441 tgatgccact ggccatgtag agaagggcgc ggccagcgtg aggtctcatg caatgaggag
     4501 gtatgactgc tcgaacggca gacaatgact tttgcattat cttgctccgg cctctttgag
     4561 agaggccgat ggctttccgg agtcactctc ctgacccagt gtggccgcgg ggcgatagaa
     4621 aaccactggt ccttcatcgc ttacaatgtg gcagctgcga aaggctcgcc agaacgcgcg
     4681 aaagatggat tcatctgact ttctgtacag atctccgagg aacggagcca tgtgagaagt
     4741 agcttcgcgc aatttatagt gagggattcc aatgaacaaa tgatgcacca tgtgagtgcc
     4801 aatattgtgg tgaaaccatt caaaaattcc gtacgagcga tccaccgaag aaagattacc
     4861 gcgcacgaaa ttccaggctt ccccgcgata ccagggaaca tccggagcgg tgtgatgaag
     4921 gaatgtgacc aggtccagcc acaccacgaa tacaaagtac ggtgcaatgt agaactttgc
     4981 aagaaatacg attccgaatt tccatccgac cacacacagg ccagcaaaga agattcccca
     5041 gagaagagtg ctcgtcagca catcccattt ttcagaaggg cgaaacagat cactgccggg
     5101 aaggaagtga gaaccgtcgc gaccagggga tctgcgaaac agatacagcg gaaaaaggaa
     5161 tagaaataca tagtatcgca agatgcgaat ggacagagtc atgtgtgaat actgagtttc
     5221 agtgactgga taccacgttt catctttgtc cacatcacca gtatgctggt ggtgagtgcg
     5281 atgactgatt ctccaaccat ggtagggcac tagaataggc gtgtgactca aatggccgat
     5341 tagattgttc aaccacttga tgtgcgagaa agaaccatgc ccacaatcat gacccacaac
     5401 gaacaaagcc cagaacatgg ttccctggag aaaccagtag actggataga aataccagct
     5461 gtccagatag agagcgagag aagcaaggcc ggcgatggtg ctgatgtcga agaagaaata
     5521 gaacagggac ttccatacgg acggttgaaa acagtgattg ggaatgcttg cgcgaatttg
     5581 agagatcgat gggactacgg gtggctgcat tatgatcctt cgagttcgac caggttcaga
     5641 tccggtgaca ccagatctcc aaccttgcag tgaacggcct ttactttgcc gctcaaagtc
     5701 gctttgattg cgttttccat tttcattgct tccacaatta caatggtctg atccttctga
     5761 actgaatcgc ccacggccac ttgcacggca atgacttttc ccggcatagg acttctgtga
     5821 agattcgatg tgtttgtttc aaggtctgca ggagaaacag acttcattcg aaaggtctgg
     5881 cctgcgatat gaacatagac ttcgtcgccc gcgaagtaga atcgtccttt cgtttcgccc
     5941 gcggctgtct gaatggaaaa agtatctcca ttcaaaatct gtgctgtttc tacaggcagc
     6001 tttatgtctg gtttgccttc ttccgagatt ccgatgtaga atccttcgcc ctgtttctgc
     6061 agctcgattg taaaggggat tccgtcctgg acgaaggtct tgatcatgtt cgtaactcgc
     6121 acggcctcgg aggctgtcga ctcaatcatg aaatgaattg cgcggctgca agcccgcagt
     6181 aaccgggtct gtggacaata gcctttcaga acaatgggcc agactgcagg atatcacccg
     6241 cactctgcgc gagccaggcg gatgcaactg ggatcgcgcc caggatgcgg agtctatgcg
     6301 cgtctatttg ctcgaagaag catatgaagt cattgaggca gtcaacagaa acgatcccgc
     6361 tctgattcgc gaagaactgg gagatttgct cttcattgtt tttttctatt cgcgcctttt
     6421 cgaagagaag ggagtctttt cagttcagga tgtgatgcgc gacgtttcag ataagctcgt
     6481 tcgcagacat cctcatgttt ttggtgagca aaaggatcat agcgttcagg aagtacttca
     6541 aaactgggaa aaaatcaagg caaaagaaaa atcctctgaa agagaggcct ttttgcccgc
     6601 acttctgcgt gcgttcaaag tgcaggagaa agcagcgcgc aaaggtttcg actgggaaaa
     6661 cgcaagagat gttcttccgc atctagaatc tgaagtggcg gagtttcgtc aggcagttgc
     6721 agacggagaa agttctgcca ggcgcgaaga ggaaatcggg gatatattgt ttacaatcgt
     6781 aaactgtttg cggcatttag gaattcaccc ggaaacggct cttcacagat ccacggataa
     6841 atttcaggat cgttttgcgc gaatgacaga atccgcaggc gaaggattca tggatctcag
     6901 tttagaggaa aaggaattac tctggcagaa atcaaaacaa aaatagatct gggcccttac
     6961 accacgatcc atctgggcgg acccgcagat ttttttctag aggcaaactc agaccaggaa
     7021 ataatccagg ggcttgcgtt tgcagaagat aagaacttgc gagtgcgaat tctcggcggt
     7081 ggttccaatg tgattttctc ggacgctgga tttcgcggac ttgtcattcg cgtggccact
     7141 cgcggaattg aactggaaag ggaaaccaat cacgggtctg aacttagagt ccaggcgggg
     7201 cacgaatggg acgcattcgt agagcattgt gtgcgagcca acctttccgg tgtagagtgt
     7261 ctctccggca ttccgggaac agcgggtgga gtgcccattc agaatgtggg agcctacgga
     7321 caggaagcaa aagagacgat cgtttctgtt cgagtttatg atagaaatgc gaagagcatt
     7381 atttctattc cgggcacaga gtgtgagttt ggttacagaa ccagtcgctt taagcacaga
     7441 gaagcagggc gattcatcat tctggatgta ctctttgcct tacgaaatga ttccagtccg
     7501 ccccgttatc cggaattgat caccaaaatg caggatccag caatctctgg gatttcgcct
     7561 catcctctgg ccgtgcgcac ggctgtgcta tcactcagga aagcaaagtc catggttcat
     7621 tctgcagaag atccggattc catttcctgc ggctccttct tcaccaatcc agtcctggat
     7681 gcagagagtc tggctaaact cgaggcaaaa atgaagtcca gcggcctgac tatgccgcgg
     7741 gcgtacccgg acggacacaa cttcaaagta tccgctgcgt ttcttgtgga gaatgctgga
     7801 tttcacaaag gactcgtgcg tgggggtgcg ggcgtttcca caaagcacgc tcttgctctt
     7861 atc
//
LOCUS       DAORFF010000113         7830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4599_length_7830_cov_4.312669, whole genome shotgun sequence.
ACCESSION   DAORFF010000113 DAORFF010000000
VERSION     DAORFF010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4599_length_7830_cov_4.312669"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="PKK76_06255"
     CDS             <1..1332
                     /locus_tag="PKK76_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNL68107.1"
                     /translation="RPKQKRESGWGAFKNEFDWEIYKEHLFLDVGLEARRYEYQNNGQ
                     ATYLVNPNNPSPDPYKSVNPDFVNLPVHDSQMTSYNTGYTMLTLKAWGFEAKPGVRVD
                     YFGLTKQRVADPRGTLSYTAPWKMTISAGGGVYHRLPDAETYSRSSGNPNLKMERAEH
                     YGGGIEQIIESWTLKAEAYRHYFTDIVVADPYITTPVRLNQDPFNRYTTPYIFNAPLN
                     FSNDGTGFSEGWELYIKKSKPDNKNGLYGWISYTWSRTLRNNHQHIITDDEKRQIYSA
                     DEARIIHEYDNTKDHYADFDRTHIANLVVGWKMSREWQIGARWKYMTSETFTPIIADD
                     KGRQIQNGRYVFNPTYSQNLNSGRLKPYHRLDVRIDHFINYSWGFGNIFVEMLNVYLR
                     DNPVNMSWNQARPISRTNPSIQNDFGTLESSTGDGKKIKFPLINFGLEVQF"
     gene            1332..1877
                     /locus_tag="PKK76_06260"
     CDS             1332..1877
                     /locus_tag="PKK76_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68108.1"
                     /translation="MNAQTKKLPLVLAALSLCALFACSLDSRDEKKEKDLELQRWIYL
                     ALTTSRDVTGSCIAAETQGIACASAAGQTVTYGITISSVYGTSSSSANAATLCNDIPN
                     SALYTSPSANFSAAAKICHFGCYRSYFQNQITQNRCASQFTSIMTTYTNCLPLAWLVT
                     CTDTTLYNCLNSCFRTGTVLP"
     gene            complement(1955..2620)
                     /locus_tag="PKK76_06265"
     CDS             complement(1955..2620)
                     /locus_tag="PKK76_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNL68109.1"
                     /translation="MIRFILSAALLFALTAPAFAQDSTCYIFNRIPVTGVDEIAKQLS
                     DPKVRLEDKSCAVSRLGELSRELVNNKNVAPSSIYNPILGSLAPQTQTADHHILREAA
                     CQALARFSDLPESERLIQPLGKVVKEDSHEEVRLACARALSRFYKDAAGATTELVAAL
                     NSELERGPQADNVTVATAIIRSLGSLRDRRSFVPLMRVIQSRFPTSTKREAQRALENI
                     KWD"
     gene            complement(2617..3111)
                     /locus_tag="PKK76_06270"
     CDS             complement(2617..3111)
                     /locus_tag="PKK76_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNL68110.1"
                     /translation="MEEEQKNISKRWRYYIDKSFQNQFMARFALVIVLVTVATLGVLA
                     VLFENPYDLLPDAGVLHSMEIKRIPQPDGTFVETPVPGKTYNAFELFWKPILFISLLN
                     LIIIVVFSLFYSHSMAGPIHNIKVSLKDLAEGGKPKAIRIRKSDQFQDLAELLNEVIE
                     KRVK"
     gene            complement(3222..6719)
                     /locus_tag="PKK76_06275"
     CDS             complement(3222..6719)
                     /locus_tag="PKK76_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL68111.1"
                     /translation="MNLKSILGSAVVLTALSCATAPPPTISNAREDAGANTEAHRQSR
                     EWLNKANRQFQDGKFEESLASVRKSLQLEESFDGYFLEGNDLMKLGDQEEALRPYLKA
                     EKLNPKNEQLGLFLAMLYTSRGQLDQAQIRYVRLMDSYPEEPLYAFKAGTGYKLMKDY
                     NRAYSTLKEADKPAFKNRDQAYLQLGDVCVELKKYDEAEMYFKKAREVNPKLGDASIG
                     SAAGKTAKLLEEGNALFRQKRFADALTKFDEAKKESPTSTAPWYLAGSCRYALSQYPA
                     AQQDLQQMLKLQPENTDGLLLLSSALQKQGKHVEAEKIIRGEIKKSPGNAELYNRLGS
                     IHADQGLKKQAIEAYHRSLTLQPNYVPARINLAFAYLDDGRYSDAEREFQNALAQDPQ
                     NADLQKGKQLVGVYRALDLGDKNFRARKFDKAIADYNTALKIRTDLPVVTNSIGRAEF
                     EKRAFPAAEAQFKKSLALDPENTAAMVGLVKVYRAQGKKADADSYSAKVAAIAKSNPA
                     RAVELGRLKEEENPKEAEDYYSKLLKERPDDTAVRTRLASLYYKQGVDANKREQYDQA
                     RTLFKKSKDLAEVQGVDEALSIVEDNIAHAPLLPGLKRAEDLYNRGRFDEALPLYEQV
                     YSRWNKSLILVKIASCKMELGRKEEAIRALNDAAEKRPEDLELTEAVFTYYLTLGETD
                     RAKAGFEKILEQHEEAYYSLYKLGIIDLRAKSYKPAVEYFNRSLIYRPDFVEAKIARG
                     VARYQSGERDVARADFEEAMAQKSELAHLNVGMMQFNDNMLDQAQKIFEDLATKNPDY
                     PEPHYHLAYIHYSRGDLDKAEKEILQARKERDDPADLAAHAKILEKKYEQSRSPELAN
                     RIKEIHKTILRKYPASANAKVSRERLAKLEGSSVPVLVPYGGTAKILTPVLYQNLVIT
                     SENKSIQAYESGSRRVVWRRFFDKPVTSLAASQAIFSLEGETIHILDPDNGSDLGTLR
                     AEAGAKLLADSDRLLAVGPKGSASLYSLTDRIAVVAPEQVRFFLAGGVVYRLRGNNLE
                     RLNDKLAVEKSVAVKGGVITVAASGLLTLVLVPGELVIYDAALSKVKSIPVGRGVDHL
                     SVASTGFLAYSSTKVIQFDPDGDKKAERNGEAIQSAALLGDGRVVLVDGQEVRILDSA
                     GKQVWSSVLGQEKIHSLFY"
     gene            complement(6716..>7830)
                     /locus_tag="PKK76_06280"
     CDS             complement(6716..>7830)
                     /locus_tag="PKK76_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68112.1"
                     /translation="KTTNEFTIAARLKEEEEGVKIQFRIEPAAAPEQLEISHTEGISI
                     LRSPSSLRVASDTLRIEEIHNMLTDSIMGTLDVQSSSAETVVSLKGSSEISVGPAPVS
                     KKVLEGSYTLIAARKGHAPQQLPVKIFAGEKRRVFIAWPDDPASATTAFVSAPTGLRL
                     AIDDDVKGPTPIYLLNESAHKVEFARKLADGRFEVVGTANPSGDDRNRAMLLKYTEAF
                     MEGYLESDIWQMVTEKSVKPLKGIRSRPFIVDEQKTTVSFPLSEGSAFIAWVSDQDSI
                     FVERIGNVFTVQGGKGSAMSGPKKAFQPIKEKPDRTILIEYNSKKSIVTVELDGTSIF
                     EGPFRPSATGQIVILGPDQMPIKKLDVRTGRGVYEE"
BASE COUNT         1912 a         2014 c         1887 g         2017 t
ORIGIN      
        1 agaccaaagc agaaaagaga gtccggctgg ggtgctttca aaaacgaatt cgactgggaa
       61 atatacaaag agcacctgtt cctggacgtg ggcctggaag cacgcagata cgagtaccag
      121 aacaacggac aggcaacgta tctggtaaac ccgaacaatc ccagtccgga tccatacaag
      181 agcgtaaacc ctgactttgt gaatcttccc gtccacgata gccagatgac atcctataac
      241 actggttata cgatgctcac tctcaaagcc tggggcttcg aagcaaagcc aggcgttcga
      301 gtagactatt ttgggcttac aaagcaaaga gtggccgatc cgcgcggcac tctgtcctat
      361 acagccccgt ggaaaatgac catcagtgcg ggcggtggag tgtatcatcg tttgccggac
      421 gcggaaacct attcacgctc ttcgggcaat ccgaatctaa aaatggaacg cgcagagcac
      481 tacggcggag gaatcgaaca gatcattgaa tcctggactc taaaagcgga agcgtatcgt
      541 cattacttca cagacatagt ggtggccgat ccttacatca caacacctgt gagactcaac
      601 caggatccat tcaatcgcta tacgactccg tacatcttca atgcacctct gaacttctca
      661 aatgacggca ctggcttctc ggaaggctgg gaactttata taaagaaaag caaaccagac
      721 aacaagaatg gtctgtacgg ttggatttcc tacacctggt ccaggactct tcgcaacaat
      781 catcagcaca tcatcacaga cgatgaaaaa cgtcaaatct acagcgcgga tgaagcacgt
      841 atcatccacg aatacgataa taccaaagat cactacgctg attttgatag aacacacatt
      901 gcaaatctcg tggtcggttg gaagatgagc cgcgaatggc agattggtgc gagatggaag
      961 tacatgactt ctgaaacatt caccccgatc atcgcagacg ataagggcag gcaaattcag
     1021 aacggaagat atgtattcaa tcctacctat agccagaacc tgaactcagg aagactcaag
     1081 ccttaccaca ggctcgacgt tcgcattgat catttcatca actactcgtg gggctttggc
     1141 aatatcttcg tggaaatgct caacgtctat ttgagagaca atccagtgaa tatgtcctgg
     1201 aaccaggcca gaccgatatc aagaaccaac ccctcgatcc agaatgactt tggaactcta
     1261 gaatcctcca caggggatgg caagaagatc aaattcccgc tgatcaactt tggccttgag
     1321 gtgcaattct aatgaatgcg cagactaaaa agctccctct ggtgcttgcc gcgctttctc
     1381 tgtgtgctct attcgcctgc tctctggatt cccgcgacga aaagaaggaa aaggacctgg
     1441 aactccagcg ctggatctac ctggctctca caacgtcgcg tgacgttacg ggaagctgca
     1501 ttgcagcaga aacacaagga atcgcctgcg cctctgcagc cgggcaaacg gtgacttacg
     1561 gcattactat cagctctgtg tacggtacaa gctcctcgag tgcaaacgca gccactctat
     1621 gcaacgacat tccgaattct gctctctaca caagtcccag tgcgaacttc tctgcagcag
     1681 caaagatctg ccatttcggc tgctatcgct cctattttca aaatcagatc acacaaaaca
     1741 gatgtgcgtc tcaattcaca tccatcatga ccacttacac caactgcctt cctcttgcat
     1801 ggctggtcac ttgtacagac actacccttt acaactgtct gaattcatgc ttccgcaccg
     1861 gaactgtgct accgtaagga cataaaaaaa cccggccatt gaccgggttt cgcatttgag
     1921 tagctagaat gctactggca aagcaattcg cgcatcagtc ccatttaata ttttcaagag
     1981 ctcgctgagc ttcgcgcttg gtagaagtag ggaacctgga ctgaatcact ctcatcaaag
     2041 gaacaaacga tctacgatcg cgaaggcttc ccagagaacg aatgatcgca gtggctacag
     2101 tgacgttgtc tgcctgggga ccacgctcca gttcgctgtt gagtgcagcc accagttcag
     2161 tagtcgcacc cgccgcatct ttgtagaaac gagacaaggc gcgcgcacat gccaggcgaa
     2221 cttcttcatg agaatcttct ttgaccactt tgcccagagg ctggatgagt ctttcgcttt
     2281 caggcaaatc tgagaaacga gccagcgcct ggcaggctgc ttccctgaga atgtgatgat
     2341 ctgcagtttg cgtctgcggt gccagagatc ctaaaatcgg attgtaaatg ctggaaggag
     2401 cgacgttctt gttgttcaca agttcgcggg agagctctcc cagtctagaa acagcgcagc
     2461 tcttgtcttc gaggcgaacc tttggatctg acaactgctt tgcaatttcg tccactcctg
     2521 ttaccggaat gcggttgaaa atgtagcaag tggagtcctg cgcaaatgca ggagctgtaa
     2581 gcgcaaataa aagcgccgct gaaagaataa aacgaatcat ttgaccctct tctctatgac
     2641 ctcgttcaac agttctgcaa gatcctggaa ctggtccgac ttacgaatgc gaattgcttt
     2701 gggctttccg ccctccgcga gatctttcag gctcaccttg atgttatgga tggggcctgc
     2761 catactgtgc gaatagaaca gactgaagac tacgatgatg atcagattga ggagactgat
     2821 gaaaagaatc ggtttccaga aaagctcaaa tgcattgtac gttttgccag gtaccggagt
     2881 ttccacgaag gttccgtctg gctgaggaat gcgtttgatt tccatgctat gaagtacgcc
     2941 cgcatccggg agtagatcgt atgggttttc gaacaatacc gcaagcacac cgagagtggc
     3001 aacggttacc aggacaatca cgagcgcgaa acgcgccatg aattgattct ggaatgattt
     3061 atcgatgtaa tagcgccagc gcttgctgat gttcttctgt tcttcttcca cgtgcatctc
     3121 ccccacgaga ttccggtctt ttccagggta ctgtcaatca attttcggca accagcctcc
     3181 cggaatcgag aggaggctgc ggcgaaaacc gaatgggatc atcaataaaa cagagagtga
     3241 atcttttcct gaccgaggac agaactccac acctgcttgc ccgcactgtc caggatgcgg
     3301 acttcctgac cgtccacaag aacgacacgt ccatcaccca gaagtgcagc agattgaatg
     3361 gcttctccat ttcgctccgc cttcttgtca ccatcgggat caaactgaat tactttagtg
     3421 gaggagtatg ctaaaaaccc tgtgcttgcg acagaaaggt gatccacccc acgaccgact
     3481 ggaatgcttt tcactttaga taacgcagca tcgtaaatga ccagctctcc tggaaccaga
     3541 accagagtca gtagtccgga tgcagctaca gtgatcacgc cacctttcac tgcgacggat
     3601 ttctctactg caagtttatc gttcaagcgc tccagattgt tcccgcgcag acgatagacg
     3661 actccgcccg cgagaaagaa tcgaacctgc tccggtgcca ccaccgcgat gcgatctgtg
     3721 agagaataga gagacgcaga acctttcggg ccaaccgcaa gcaatctatc tgaatctgcc
     3781 aggagctttg cgccagcttc tgcacgcaga gttcccagat cacttccatt gtctggatcg
     3841 agaatgtgaa tcgtctctcc ctccagagag aagatcgcct gcgatgcggc cagagaagtc
     3901 acaggcttgt caaagaaacg gcgccatact acgcgacggc ttccggattc gtacgcctgg
     3961 atgctcttgt tttcagacgt aatgaccagg ttctggtaga gaacaggagt cagaatcttt
     4021 gcagttccac cgtacggaac gagaacaggt acgctcgatc cttccagttt agccaggcgt
     4081 tcgcgagata ctttcgcatt cgcagacgct ggatacttgc gaaggatagt tttgtggatt
     4141 tctttgattc tgtttgcgag ttcaggactt ctggactgct catatttctt ttctagaatc
     4201 tttgcgtgcg cagcgagatc tgcaggatca tccctttctt tgcgagcctg aagaatttct
     4261 ttttcagctt tgtccaggtc accgcgtgaa tagtgaatgt acgccagatg gtagtgcggt
     4321 tccggataat ccggattctt tgtggccaga tcttcaaata tcttctgcgc ctgatcgagc
     4381 atattgtcat tgaactgcat catgcccaca ttgagatgag caagctcaga tttctgggcc
     4441 atggcttctt cgaaatcagc gcgcgcaacg tcgcgttcgc ctgactgata acgcgcaaca
     4501 ccacgagcga tctttgcttc tacaaaatca ggtctgtaga tgagcgacct gttgaagtat
     4561 tccactgcgg gcttgtacga ttttgcgcga agatcaatga tcccgagttt gtaaagagag
     4621 tagtatgctt cttcatgctg ctccagaatc ttttcaaacc ccgccttcgc gcgatctgtt
     4681 tcccccagag tgaggtagta tgtgaacact gcctctgtca gctcgagatc ttctggacgt
     4741 ttttcagcgg catcattgag cgcacgaatg gcctcttcct tgcgacccag ttccatcttg
     4801 cacgaagcaa tcttcactag aatcaaggac ttattccacc tggaatacac ctgctcgtag
     4861 agaggaagcg cttcatcgaa ccttccacga ttgtagagat cttctgctct cttgagtcct
     4921 ggaagaagag gtgcgtgtgc gatgttatct tcaacgattg aaagagcctc gtccacgccc
     4981 tgcacttctg ccagatcctt tgacttctta aatagagtgc gcgcctgatc gtactgttct
     5041 ctcttgtttg cgtctacacc ttgcttatag tagagagaag caaggcgggt gcgcacagcc
     5101 gtatcatcgg ggcgttcttt gagcaatttg ctgtaatagt cttccgcttc ttttgggttt
     5161 tcctcttcct tgagacgacc gagttccacg gcccgggcag gattgctttt tgcgatcgca
     5221 gccaccttcg cgctgtagga gtctgcatcc gctttctttc cctgtgcgcg atacactttt
     5281 acaaggccaa ccatggcagc tgtgttctct ggatcgagag ccaggctttt tttgaattga
     5341 gcttctgctg cggggaatgc tctcttttca aattcagcgc gaccaatgga gtttgtgact
     5401 acaggcaaat cggttctgat tttgagcgcg gtattgtagt ccgcgattgc tttgtcgaac
     5461 ttgcgtgcgc gaaaattctt atctccgaga tccagggcgc gatacactcc aaccagttgt
     5521 ttcccctttt ggagatccgc attctgtgga tcctgtgcca gagcattctg gaattcgcgc
     5581 tccgcgtccg aataacgtcc gtcgtccagg taggcaaacg caaggttgat tctggcagga
     5641 acatagtttg gctgcaaagt cagtgagcga tgatacgctt caattgcctg ctttttcagg
     5701 ccctgatctg catgaatgga gcccagacga ttgtagagct ctgcattgcc cggcgatttt
     5761 ttgatttcac cgcgaatgat cttctctgct tccacatgtt tgccctgctt ttgcagcgcg
     5821 ctcgaaagaa gtagtagccc gtctgtattt tcaggctgaa gcttcaacat ttgctgaaga
     5881 tcctgttgtg ccgcaggata ctgagaaagc gcataacggc aagatccagc cagataccac
     5941 ggagccgtgg atgtgggaga ttccttcttt gcttcatcga acttagtcaa agcatccgca
     6001 aatcgtttct gacgaaagag agcgttgcct tcttccaata attttgcagt cttgcccgcg
     6061 gcagatccga tcgacgcatc tccgagcttc gggtttactt cgcgtgcctt tttgaaatac
     6121 atttcggctt catcgtattt cttgagttca acgcaaacgt ctccgagctg cagatatgcc
     6181 tggtcacgat ttttgaatgc aggtttgtcc gcttctttta aagtgctgta agctctgttg
     6241 taatctttca tgagcttata gccagtgccg gctttgaatg cgtatagagg ctcttccgga
     6301 taggagtcca tcaatcgaac atagcgaatt tgcgcctggt cgagctgacc acgcgaagtg
     6361 tagagcatgg ccagaaacag tcccagttgt tcgttcttcg gattcagttt ctctgctttg
     6421 aggtaaggtc tgagagcttc ttcctgatct cccagcttca tgagatcatt tccttccagg
     6481 aaataaccat caaaactttc ttcgagttga agactctttc tcacgctggc gagggactct
     6541 tcaaactttc cgtcctggaa ctgacgattt gctttgttca accattctct ggactggcga
     6601 tgcgcttccg tattggcgcc agcgtcttcg cgagcattag aaatggtggg tggaggtgcc
     6661 gtggcacagg aaagtgcagt cagtacaact gcagagccaa ggatggattt aagattcatt
     6721 cttcgtatac ccctcgtccg gtgcggacat cgagcttctt gatcggcatc tggtctggtc
     6781 ccaggataac gatctggccc gtagcagaag gacggaatgg tccttcgaaa atggaagttc
     6841 catccagctc aactgttacg atggacttct tactattgta ttctataagg atggttctat
     6901 ccggcttttc tttgattggc tggaatgctt ttttgggacc agacatggca gagccttttc
     6961 cgccctgcac tgtgaataca tttccgattc tttctacgaa gatagaatcc tgatccgaaa
     7021 cccacgcaat gaacgctgac ccttcactga gtgggaaaga aacagtggtc ttctgctcat
     7081 ccacgatgaa tgggcgagag cgaattccct tcaaaggctt cactgatttc tcagtcacca
     7141 tttgccagat atcgctttcc agatatcctt ccatgaacgc ttcagtgtat ttcaaaagca
     7201 tggcacggtt tctgtcgtcg cctgacggat tggcagtgcc cactacttca aatcttccgt
     7261 ccgcaagctt gcgcgcgaat tccactttgt gcgcggattc attgagcaaa tagatgggag
     7321 tcgggccttt gacatcatcg tctatggcca ggcgaagccc ggtcggtgca cttacaaaag
     7381 cagtggtcgc agaagcagga tcatccggcc atgcaatgaa cactcgcctc ttttcacctg
     7441 cgaatatttt tactggaagt tgctgaggag catgcccttt tcgcgcggcg atcagagtgt
     7501 aggagccttc cagaactttt ttagaaacag gggccggacc aacactgatc tcagaggatc
     7561 ctttcaggga tacaacggtc tcagcggatg acgactgcac gtcgagagtg cccatgatac
     7621 tatccgtgag catgttgtgg atttcttcta tgcgaagagt atcgcttgca acgcgcagac
     7681 tcgacggaga acgcaggatg gagatgcctt ctgtatgcga aatttccaat tgctccggag
     7741 cggcggcagg ttcaatgcga aattgaatct tcactccttc ctcttcttct ttcaggcgag
     7801 cagcaattgt gaattcgttt gtggtcttgc
//
LOCUS       DAORFF010000114         7817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4615_length_7817_cov_3.891394, whole genome shotgun sequence.
ACCESSION   DAORFF010000114 DAORFF010000000
VERSION     DAORFF010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4615_length_7817_cov_3.891394"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PKK76_06285"
     CDS             <1..626
                     /locus_tag="PKK76_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HNL68113.1"
                     /translation="EHGGDCIDCNMCNLVCPTGIDIREGLQVGCIACGLCVDACTNVL
                     SKFEKRTLIDYRTIEQVENPDAHRKYLRPRTVVYASLLLCFATAFVYLLVTRIPMYAT
                     VLRDRAISALYVPDIGYQNGYEVHVGNQSRKPIEVKIMVLDKRYEILAPQDTYKIGAE
                     GFEKIRFVVRSAQKEEFARTRPIEFKIVDVNEPTHERVVQTVFSYPI"
     gene            673..1173
                     /locus_tag="PKK76_06290"
     CDS             673..1173
                     /locus_tag="PKK76_06290"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNL68114.1"
                     /translation="MSKSMRIAFIMISLLLGATVASGIWFVKYAMSVQEPVMDDAYFA
                     KGLNYQNRVASLDRAKAQGFAIATNLPANAPLSRSKQQLTFTVSGKTPIDNATLHIVI
                     ERPATTRERKILDLSFAQSAKISETERKFQTEVDFPGSGLWEISAEAAVNKDMAVYTR
                     QTVTVQ"
     gene            1173..1313
                     /gene="ccoS"
                     /locus_tag="PKK76_06295"
                     /pseudo
     CDS             1173..1313
                     /gene="ccoS"
                     /locus_tag="PKK76_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
     gene            1418..2080
                     /locus_tag="PKK76_06300"
     CDS             1418..2080
                     /locus_tag="PKK76_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNL68115.1"
                     /translation="MIEAVFAGAFVQGLTGSLHCPGMCGPFAHAFGRSGRISVSILYN
                     VMRTISYGLVGYLLGTSGGAINHLFESGIAAIVGGCLLVLLGLGLVFPRFSVHLRMPD
                     LFLKMIGNVMRSSTGAYAGPAFLGAISGFMPCGVLWPAYALALGTGVPWVGAVVMVFF
                     SFGTYPAMLAMGLSGGVWREKLSRPVYRVVFAILMIGLGIYTIVAHAFFSEICRSVAW
                     QS"
     gene            2068..4497
                     /locus_tag="PKK76_06305"
     CDS             2068..4497
                     /locus_tag="PKK76_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNL68116.1"
                     /translation="MAVITEKTKCYHCGNDATDTTFVLEVDSEKRAFCCSGCLTAYRI
                     IHEGGIESYYSHRDAYAPTVREDRDAGFYQAWEKRIPLRDGIRSGLFLIEGIHCASCV
                     WLNERVILAVGGVISASVQLSTSRAKISWNDSDTSLQKIAEALAKIGYRLVPIEETLE
                     SASRRQARGLLRRMTVAGFFAGNVMLLSVALYAGYFGSIDRYSKNFLQLASLMMTIPV
                     VLYSASVFFKNAYHALRNRTLSMDVLTATGISLAFSYSLYVYFSEVGEVFFDSICFVV
                     FIILTGRFIESRLRLKGLYYVENIRKSAAKTARVDREGWRTVLVDEVKKGDLVDVAAD
                     EMVPVDGVLRSENAEVEESMLTGEARPVYKRAGDLILSGTRALTQLRMECSAVSSESA
                     LARISALAEESMDQAPRVLRFTDRASRFFIAFALFAAVFTFSYWYFFKHDVHSAILNM
                     ITLLIAACPCALSLAVPTVFTVSVQKSFDLGCLLKNGSTLELLSRAKAIAFDKTGTLT
                     RGEPEIVQVSVVDDIFSEEYVCALASELQRRADVRHPIARAFYSLQRLTGSSANLPDL
                     GRVEYKPGLGIVSSSPEASYALGSRRLMESVGVECQPAGAPGSISIYLARLGGRVPEL
                     VSVFVLADAIRPEASDVLRRLSRNSSLFLLTGDTVENAAQVSKQLGITEIYAEKTPEQ
                     KREIIQARQASGITIMVGDGINDSVALAQAHAGVSFANASEISLHSADVLLLRNDLRQ
                     FLSLVQLSRRARSIVLQNLGLSFLYNMFLIPMACAGWIIPLAGALFMSLSSLTVVLNS
                     LRLLSFRKEIG"
     gene            4538..5449
                     /locus_tag="PKK76_06310"
     CDS             4538..5449
                     /locus_tag="PKK76_06310"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HNL68117.1"
                     /translation="MRKVALCLVLVSAFISSVDAENLVAVPQLQTRVTDLTGTLSASE
                     NAELSKILADYEKEKGSQIAVLILPSTRPETIEQYSIRVAEQWKIGRKGVDDGVLLIV
                     AKEDRKLRIEVGYGLEGVLPDAKAKDIIDEVIVPYFKNGDFYGGVKIGVETIIATIKN
                     EPLPPPKFKPDAKFAKQAATAIGMGIVVSLIVGAILSALPVIRHLIALTIYLVILYFV
                     GLLFGSSMASLVHAGITGLFLLGVRYLGGTSAGKAWNSDSSSSWSSSSSSSSSSWSSS
                     GSSSDSDSSSTSGGGGSFGGGGASGSW"
     gene            complement(5476..5856)
                     /locus_tag="PKK76_06315"
     CDS             complement(5476..5856)
                     /locus_tag="PKK76_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68118.1"
                     /translation="MIRTLFLFWLGLATLIEFGLALGGLFAPDFLRAQFHIGTTEDTR
                     FLTYVVAWLILMIAIACALAWRWSFLGKSAGYVLTNWLGLFWIGIGIGIYFQSNGVRT
                     DNLALDSAKGAILILLNYLNDRFG"
     gene            5938..>7817
                     /locus_tag="PKK76_06320"
     CDS             5938..>7817
                     /locus_tag="PKK76_06320"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNL68119.1"
                     /translation="MQANIQITGMTCATCALRIEKGLSKMPGVENATVNFATGSAHVT
                     YADHQVATDAMLARIHDLGYEGVIPNEESEITQIKQKEVRKLRRRLILSAVLSAPLLW
                     TMGSHIPGLHIFVPQILMNPWVQLLLASPVQFIIGFAFYRGAFRALSNATANMDVLVA
                     LGTSAAFFYSLFLALSGHSHQGLYFETSAVLITLIVFGKFLESVATGRTSGAIEKLMK
                     LQPDRASVVRDGTEIQISLDQVVKGDRINIRPGERIPVDGRVIDGDSHVDESMFTGES
                     VPVHKRKGDSLLAGTMNTNGALQMEADKVGRDTALAAVVRAVEEAQGSRAPIQRVADQ
                     ISSVFVPVVVLVALAAGVLSYLIFVPGEFASALERAIAVLVIACPCALGLATPASIMA
                     GTGRAAEAGVLFRGGEHLEAAGALDLIILDKTGTVTMGQPALTEVTGEIPESEILRIA
                     ASAETSSEHPIAQAIVRAASERSIVTSPVSGFKATPGQGIAAELEGKEILLGTEAWLA
                     QSNISPGDGTASAILESQGKTVVYLAVDGRYAGLLAISDQLKPSSAAAIQRLHELGLE
                     TYMVTGDNERAARWIASQCGIDRVAARVLPEGKARIVSEMKQAGRKVGMVGDGINDAP
                     ALA"
BASE COUNT         1796 a         1824 c         2139 g         2058 t
ORIGIN      
        1 ttgaacacgg cggggattgc atagattgta atatgtgtaa cctggtttgt cctactggaa
       61 tcgacatacg cgaaggcctt caggtgggtt gcattgcctg cggactttgc gtggatgcgt
      121 gcacgaacgt tctgagtaaa ttcgaaaaac gcactctgat tgactatcgc acaattgagc
      181 aggtggaaaa tccggatgcc catcgcaaat acctgagacc gcgcacggtc gtgtatgcgt
      241 cgcttcttct gtgctttgca actgctttcg tttatctact cgttactcgc attcctatgt
      301 atgccacagt ccttcgtgac cgcgcgattt ccgcgctcta tgtgcctgac attggctacc
      361 agaacggata tgaggttcat gttggaaatc aatccaggaa gccaattgaa gtgaaaatca
      421 tggtcctgga caagcgttac gaaatactgg cacctcagga cacttacaaa attggggcgg
      481 aaggatttga aaagattcgc tttgttgtaa gatctgcaca gaaggaagaa ttcgcacgca
      541 ctcgccccat tgagttcaag atcgtggacg taaacgaacc aacgcatgag agagtggtgc
      601 aaactgtgtt cagttaccca atttagaagt gatagaaacg gaccaggaat caaagaggtc
      661 aataaacctg ctatgtcaaa atcaatgaga atcgctttca tcatgatttc cctcctgctc
      721 ggtgcaaccg ttgcgagcgg aatctggttc gtgaagtatg caatgagcgt tcaagaacct
      781 gtgatggacg atgcgtattt tgccaaaggc ctcaactatc aaaatcgagt ggcttccctg
      841 gacagggcaa aggcacaggg atttgcgatt gccacgaatt tgcctgccaa cgctccactg
      901 tcacgttcaa agcagcagct gacttttact gtttcgggga aaacgcccat tgataatgcc
      961 accctgcaca ttgtaattga aagaccggcc accacgcgcg agagaaagat ccttgatttg
     1021 agtttcgcgc agtctgcgaa aatttcagaa acggaacgta agttccagac agaagtggac
     1081 tttccaggat ccgggctctg ggaaatttca gcggaagctg cagtgaacaa agacatggcc
     1141 gtttatactc gacagactgt gacggtgcaa tgatgcctgg ccttcttttc atgcttccga
     1201 ttgcaatcgg cattgcgctg atttttctgg gattcttcct ctggagtgtg cgcaacgggg
     1261 actacgaaga tatggaaatg ccggcgtaca agatgacctt tgatcaggat gaaggcgaca
     1321 aatccattca gacttcgaac ggcgtttcgc cggtccacga agcaggttcc ctggcggttg
     1381 ctcaacctgc cgacgcgccc gatcccggga aatagaaatg attgaagccg ttttcgcggg
     1441 agcgtttgtt caggggctta caggttccct gcactgtccc gggatgtgcg gaccatttgc
     1501 gcacgctttt ggcagaagcg gccggatttc tgtttccata ctttataacg ttatgcgcac
     1561 tatctcgtac gggcttgtgg ggtatcttct aggaacttcc ggaggcgcca tcaatcattt
     1621 gtttgaatcg ggaattgccg cgatcgtggg tggttgcctt ctggtgctgc tcggtctcgg
     1681 gcttgtgttc ccgcgtttct ccgttcacct gcgcatgccg gacttatttc taaagatgat
     1741 tgggaacgtt atgcgctctt ctacgggggc ttatgccgga ccagcattcc ttggcgcgat
     1801 ttctggattc atgccgtgcg gtgtgctctg gcccgcttac gctctggcac ttggaacggg
     1861 cgtaccctgg gtgggtgctg ttgttatggt tttcttcagt tttgggactt atcccgctat
     1921 gcttgcaatg ggcctttccg gcggtgtctg gcgtgagaaa ctatccaggc ccgtctacag
     1981 agtagtgttt gccatcttga tgattggcct tggaatttac accattgtgg cgcatgcatt
     2041 tttttctgag atatgcagga gtgtagcgtg gcagtcataa ctgaaaaaac aaaatgttat
     2101 cattgtggaa acgatgcaac ggatactact ttcgtactgg aagtagattc agagaaacga
     2161 gcgttttgtt gcagtggatg tttgactgca tatagaatca tccatgaagg cgggattgaa
     2221 agttactaca gccaccgcga cgcctatgca ccaacggttc gcgaggatcg cgatgcagga
     2281 ttttaccagg catgggaaaa acgaattccg ttgcgagatg ggattcgtag cggcctgttt
     2341 ctgatcgaag ggattcattg cgcgtcgtgc gtctggttga atgaacgagt catccttgct
     2401 gttggtggag tgatttctgc gagtgtccag ctatctacca gcagagcgaa gattagctgg
     2461 aacgattcgg atacgtctct tcagaagatt gcggaagcac tggcgaagat tggctatcgc
     2521 ctcgttccta tagaagaaac actcgaaagc gcatctcgcc gccaggcacg tggacttctt
     2581 cgtcgcatga ctgtggcagg cttctttgct ggaaatgtga tgctcctgag tgtggcgctc
     2641 tacgcaggct attttggttc gatcgacaga tattctaaga acttcctcca gcttgccagt
     2701 ctgatgatga ccattccggt cgttctctac tccgcttccg tcttcttcaa gaatgcttac
     2761 catgcgctgc gaaatcgcac tctgagtatg gacgttctga ctgccacagg aatcagcctt
     2821 gcgttttcgt acagtttgta tgtgtatttt tcagaagttg gggaagtgtt ctttgattcc
     2881 atttgttttg tcgtctttat tatcttaacc ggaaggttta ttgaatcccg tcttcgcctg
     2941 aaaggtttgt actacgtaga gaatattcgc aaatctgctg caaagaccgc acgcgtggat
     3001 cgcgaaggct ggcgcacggt ccttgtggat gaagtgaaga aaggggatct tgtggatgtg
     3061 gccgcggatg aaatggtccc ggtggacgga gtgctccgca gcgaaaacgc ggaagtggaa
     3121 gagtcgatgc tcacgggaga agcgcgtccg gtctataaac gggccgggga tctcatcctc
     3181 tctggaacgc gggcgctcac gcagcttcga atggagtgtt ccgccgtaag ttcagagagt
     3241 gctctggcca gaatcagcgc acttgcagaa gagagcatgg atcaggcgcc cagggttctg
     3301 cgattcacag atcgcgcgag caggttcttc atcgcctttg cgttgtttgc tgccgtattt
     3361 actttctcat actggtattt tttcaagcac gatgttcatt ctgcgattct caacatgatc
     3421 actctcttga tcgcggcctg tccatgcgcg ctcagtcttg ctgtgccaac tgtgttcacc
     3481 gtttctgttc agaagtcttt tgatctgggt tgtttgctaa aaaacggcag cacactggaa
     3541 cttttgagtc gtgcgaaggc catcgctttt gacaagacgg ggactctgac gcgcggagaa
     3601 ccagagatcg ttcaggtgtc tgtcgtagat gatatttttt ctgaagagta tgtttgcgcg
     3661 ctcgcgtctg agctgcaaag acgcgcggat gttcgtcatc caattgccag agcattctat
     3721 tctctgcaaa gattgaccgg atcatctgcg aatttgcccg atctgggtcg cgtcgaatac
     3781 aagccaggtc ttgggattgt ttcttcatca cctgaggcga gttatgcgct cggcagccgc
     3841 agattgatgg aaagcgtcgg cgtagagtgt cagccagcag gagcgccagg tagtatttcc
     3901 atttatctcg cacgtttggg cggtcgtgtg cctgagctcg tcagtgtttt tgttctggca
     3961 gatgcgatca gaccggaggc aagtgatgtg cttcgtcgtc tatcccgcaa ttcatctttg
     4021 tttctgttga ccggggacac tgtggagaat gccgcgcaag tttcaaaaca gctgggcatt
     4081 actgagatct acgccgaaaa aacgccggaa caaaaacgag agatcatcca ggcaagacag
     4141 gcatccggaa tcacaatcat ggtgggggac ggaatcaacg actccgttgc cctcgcgcag
     4201 gcacatgccg gtgtttcgtt tgcgaatgct tctgaaattt cactccattc tgcggatgtg
     4261 ctgctactgc gaaatgatct gcggcagttt ctgagtctgg ttcaactatc caggcgtgct
     4321 cgtagcattg tgcttcagaa ccttggtctg agtttcctct acaatatgtt cctgattcct
     4381 atggcatgtg ctggatggat cattcctctg gctggagcgc ttttcatgtc tttgagttct
     4441 ttgactgtgg tgctcaattc actgcggctc ttgagttttc gaaaagaaat cggctaaata
     4501 gtttgtcgaa tgttcgctat cggattttgt tagccccatg cgcaaggtgg ccctttgtct
     4561 tgttctcgtt tctgcattca tttccagcgt tgatgcggag aatctcgtcg ctgttccgca
     4621 gttgcagacg cgagtcacgg acctgaccgg cactctgtcc gcttctgaaa acgcagagct
     4681 ttctaagata cttgctgatt atgagaaaga gaaaggtagc cagattgcag ttctcattct
     4741 accatctacg agaccggaga caattgagca atattcgatt cgcgtagcgg agcagtggaa
     4801 gatcgggcgc aaaggagtag acgacggcgt cttgctcata gttgcgaagg aggaccgcaa
     4861 gcttcgaatt gaagtaggct acggtctcga aggcgttctc cctgatgcga aagcaaagga
     4921 catcatagat gaagtgattg ttccctattt taagaacgga gatttctacg gcggcgtaaa
     4981 gatcggagtg gagactatca tcgccaccat taaaaacgaa ccattgcctc cgcctaaatt
     5041 taagccggat gcaaaatttg caaagcaggc ggccactgcg attggaatgg gaatcgttgt
     5101 gagtttgatt gttggagcga ttctctctgc tcttcccgtt attcgccatt tgatcgcgct
     5161 gacgatctat ctagtgattc tatacttcgt aggacttctg tttggttctt caatggcgag
     5221 tttagttcat gcgggtatca cgggactgtt ccttctgggc gtgagatatt tggggggaac
     5281 atccgcaggc aaagcctgga attcggactc ctcctctagc tggtcttcct ccagttcaag
     5341 ttcatccagt tcctggagtt caagcggaag cagttctgat tctgattcca gttcgaccag
     5401 tggaggaggc ggtagctttg gcggaggcgg tgccagcggc agctggtagt gctgccttct
     5461 gtcacagatt cgcgatcaac caaatcgatc gtttagataa tttagtaata tcagaatggc
     5521 gcctttcgca ctgtccagcg ccagattgtc tgtgcgaacg ccatttgatt gaaagtagat
     5581 tccaattcca atcccaatcc agaatagccc cagccagttt gtgagcacgt aacctgcact
     5641 ctttccaagg aaagaccatc tccacgctag agcacaggcg atggcaatca tgaggatcag
     5701 ccaggcaacc acgtaagtca aaaagcgtgt atcctccgtg gttccaatgt ggaattgagc
     5761 tcgaagaaag tcaggcgcga aaagaccacc cagcgccagc ccaaattcga tgagagtcgc
     5821 cagtccaagc cagaagagga acagtgttcg aatcatggat catgatggtg gccgaaagcc
     5881 aggattgtca aacaaagaag tagtatgcat tcaggtcggt gctggcttat ttcaactgtg
     5941 caggcaaata tacagatcac tggaatgacg tgcgccacgt gcgcacttag aattgaaaag
     6001 ggtttgagca agatgccggg agtggagaat gccacggtaa attttgcgac aggatccgcg
     6061 cacgtgacgt atgcggatca tcaagtggcc acggatgcaa tgctcgctcg aatccatgat
     6121 ctgggttacg aaggggtgat cccgaacgaa gaaagcgaaa tcactcagat caaacaaaaa
     6181 gaagttcgca aacttcgtcg ccgtcttatc ctttctgctg ttttgtccgc acctcttctc
     6241 tggacgatgg gatcgcatat acccggtctc cacattttcg tcccgcaaat cttgatgaat
     6301 ccctgggtgc agttgctcct tgcttcacca gttcaattca tcatcggttt tgctttttac
     6361 cgtggcgcat ttcgcgcgct ttccaatgca acagccaaca tggatgtgct ggtcgctctg
     6421 ggcacttcgg cagcattttt ctacagtttg tttctggctc tatccggaca ttcgcatcag
     6481 ggtctgtatt ttgaaaccag tgcagtgctc attactctga ttgtgtttgg aaagtttctg
     6541 gaatctgttg caaccggaag aacgtcaggc gccattgaga agttgatgaa gctgcaaccc
     6601 gatcgcgctt ctgtcgtgag agatggaact gaaattcaga tttctctgga tcaggtggtg
     6661 aaaggggatc gtatcaatat tcgtccgggg gaacgcattc cagtggatgg ccgcgtcata
     6721 gacggtgatt ctcacgtgga cgaatccatg tttacaggtg agagtgtccc tgttcacaaa
     6781 cgcaaaggtg attctcttct ggccgggact atgaatacaa atggcgcttt gcagatggaa
     6841 gctgacaaag tgggtcgcga tactgcactg gctgcggttg tgcgagctgt agaagaagcg
     6901 cagggtagtc gtgctcccat ccagcgtgtg gcagatcaga tatcttctgt ctttgttccg
     6961 gtggtggttc tcgttgcgct tgctgctggt gtcttatcct acttgatatt cgtgcctggc
     7021 gaatttgcct ctgctctgga gcgagccatt gcagttctgg tgattgcctg cccctgtgct
     7081 ctgggtcttg caacgcctgc ttccattatg gctggaacag gtagggcggc agaagctggc
     7141 gttttgtttc gcggtggaga acatctggaa gctgcggggg ctctcgattt gatcatcctg
     7201 gacaagactg gaactgtgac gatgggtcag cctgcgctta cggaagtgac cggcgaaatc
     7261 ccagagtcag agatactcag aatcgcggcc tctgcagaga cgtcatcgga gcatccgatc
     7321 gcgcaagcga ttgtgcgagc agcgtctgaa cgctcgatcg ttacgagtcc agtgtccgga
     7381 ttcaaagcta caccaggcca gggaattgcc gcagaactcg aaggaaaaga gatcttactt
     7441 gggacggaag cgtggcttgc tcagagtaat atttcacctg gagatggaac agcaagtgcg
     7501 atcctcgaaa gccagggaaa gaccgtggta tacctcgcgg tagatggacg ttatgcggga
     7561 ttgcttgcga tttcagatca actcaagcca tcatctgcgg ctgcaattca gagattgcac
     7621 gagcttggtc tggaaacata catggtaacg ggcgacaatg aaagagcagc acgctggatt
     7681 gcttctcaat gcggaataga tcgagtggcg gctcgtgtgc ttccggaagg aaaggcgcgc
     7741 atcgttagcg aaatgaaaca agcaggaaga aaagtaggaa tggtggggga cggcatcaat
     7801 gacgcgccag cgcttgc
//
LOCUS       DAORFF010000115         7800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4630_length_7800_cov_3.603615, whole genome shotgun sequence.
ACCESSION   DAORFF010000115 DAORFF010000000
VERSION     DAORFF010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4630_length_7800_cov_3.603615"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PKK76_06325"
     CDS             <1..1080
                     /locus_tag="PKK76_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68120.1"
                     /translation="VMSADDYDLVMSARDKDGSMYVTESSLGVFGSTDRAQFIHRIKN
                     FGAPEKLSWSRRAVQSMIAQLSKEKIDMFFAESSSVHTLRVDEAGTLYIGANVDHDNA
                     FIAVSKEGTSTLLASMSQLSKVANGYQTGGTASRLYVSPKGTAWLMVQGIDKPGGKDY
                     GTNAFYMRRTEEGGWRATPVPVRCGGQPCSMKVKYSIFSHGVADEGGGFVFHDGNGFW
                     RATPDGDVKNLLQLKLPGGDVRIVGPVLLSNGDIWYALSTVYSVSSYSKVDQQTGYAE
                     VRNTWFTVGDRSRFIRIRVRGGKAELGEISSEAMFAALRKKGVPLRPDSQVMKTVRFV
                     PDYATGGFIAFDAHHSVLYSILPGD"
     gene            1120..1857
                     /locus_tag="PKK76_06330"
     CDS             1120..1857
                     /locus_tag="PKK76_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68121.1"
                     /translation="MRLLLILLFASPLLAQENSPDFPEYLAQGTENIRDQRTGEVDIN
                     RVAQAAPTTTQPAAPVQTGLPGFAEIPWKATYSEVKTRLKNLSTAAVQTERVEILMEE
                     KNKSILVRRNEVLYRYNFYRTPVEVLRIANHDLTDADYEQQEGQLFQVRVTFSLIDAA
                     KIQDRIEKSHGAKTKSTVDPKKMEGAEIWELPGGVILEWVEPYRKMKFSRTVDFLSSE
                     MTKQIMKEYEDYFDAREKWIINNIKLY"
     gene            complement(1890..2666)
                     /locus_tag="PKK76_06335"
     CDS             complement(1890..2666)
                     /locus_tag="PKK76_06335"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL68122.1"
                     /translation="MANIIQFPGTRQTALQRVQSSYSLRDVQKQFGISEKLLRKWAEA
                     GAVETVPGKEGEIRFGLGALARFRSIREERTRGKTLKQIDEEMRGQLDLFASRPSGIV
                     RSLSEKRTPFEEGLILLDRGDASAASFFQKALEQDDHCADAHCNLGILAFESGDVPGA
                     MTEFIRALESEPGHFESHYDLGNLYFDAGNLPLAQMHFETAARIEPEYADTFFNLGLL
                     YARLENWKKSKECFERYTAFEDIDHGEAVKEILKHLEQQT"
     gene            complement(2696..3589)
                     /locus_tag="PKK76_06340"
     CDS             complement(2696..3589)
                     /locus_tag="PKK76_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HNL68123.1"
                     /translation="MRSIWKGHIRFSLVTIPVRIYNAIETSESIRFNQLHRECNGRVG
                     YDKKCKKCEKSLTTEEIVKGYEYEPDQYVVFEDSDFEKVKLKSTKVIEIEGFVGVDEV
                     HPTLFESPYYAGPDGDVAARSYALLCQALKDAGKVGVGKVVLRDREDVVLLSPHEKGL
                     ALYRLRYPAEVRKMAEVPQIDKVEPADKEQLKLARNLVESMARPLSDIELKDNYRGAL
                     KEMIDAKVAGKEIVQVEEVEKPAMDIMTALKASIEKAAAMRGGMIPAKGEKKEKAQAK
                     DKAEGKEKAAKAVKTRKTKAG"
     gene            complement(3645..5873)
                     /gene="ligD"
                     /locus_tag="PKK76_06345"
     CDS             complement(3645..5873)
                     /gene="ligD"
                     /locus_tag="PKK76_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HNL68124.1"
                     /translation="MAKKQTVLAGKRKLELSNLDKILYPGDGIVKAEVLQYYVTIAPY
                     MLRYVRGRPLSLVRFPDGIEGEQFFQKNRPDWVPEWLHSVKLGDIDYMLAEEDAAVVF
                     LANLAALEFHQMQMRPSVSQDADYMVFDLDPPENSNFEIVRDLALNLRPYLESLGYHV
                     FVKTTGGKGLHLIMPLLPHSYDIVFEAAKAAAIDFASRNKGVTLEIKKERRAQNILLD
                     VYRNRSGQTIVSPFSLRGKPGAPVSMPLSWEDLERARSPLDYNLRNAYDLIRQGDPWE
                     GMYAHTAVPHNARAKLAPVTAELETYKKKRSFDKTPEPAPASSAATGQSFVLHRHHAS
                     HLHYDLRLEQDGALTSWAVPRGLPPRPGIKRLAVHTENHPIEYLTFEGKIPKGQYGAG
                     EMWIFARGQYEITKQKKDGFYFRTHAEGLHGEYRMIHTRDKEWLMERLDEEPRLDFEP
                     MLATQTETVPSGDMIFEVKWDGIRALVFIEDGQITIRSRNGRDITAAFPEIAVPDSYH
                     ASSAVLDGEIVCLDGSGRPVFQNVIRRLQNGANKKLEQKFPAFIYLFDVLSLDGKRTL
                     KDPLHLRRAWVQDLIRSGGAVRFSQSYPDGQALFDAAKEHGLEGIMAKSPDSPYVLGR
                     RSTSWVKIKVRSTMDCVIIGYTEGEGGREKLGSVHVAEKTESGLHYVGKVGSGFSDED
                     INDLSARLATMEIEKPTVKVPKVEKGTWVEASLVCEVEYASKTRDGMLREPVFIRLRP
                     DL"
     gene            5954..6988
                     /locus_tag="PKK76_06350"
     CDS             5954..6988
                     /locus_tag="PKK76_06350"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL68125.1"
                     /translation="MKRFVSTCLCLLTTLSMDCAQKPSPLSIDWGLIAIAASIVKIPR
                     FVFVTSNTGNVYVYSINPFVCSLNPLSPMWAPASPGIPAIDSTGQFLFVPGSVANQIA
                     SYRINTTTGVLTQVGFVSTTSPTALAYDPAVGQVLSVSTGSSMISRFALMPDGSLTSA
                     GSVAMPAPASGNIAIDTGSRIAFVPHGISLFSAASLAAGVASSSSAPAVATSAALNGG
                     YLYVGGSTTILTYRLNGTNAPGFVSTTPSAGTLTPLAADSQGANLYGADATGVLNRYA
                     IQNGGTLLLNSQSLFGPSANSIAVDPTGTCVVMSGGSNVRAFRTSSAGLTLFAAGFVS
                     NPNAVLIASY"
     gene            7011..>7800
                     /locus_tag="PKK76_06355"
     CDS             7011..>7800
                     /locus_tag="PKK76_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004503452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL68126.1"
                     /translation="MKTVKKIFLGIAAVLAVLLVYVFVSKAITDRKMERETAMPEGGI
                     SSLESVTIGGISQWILIRGEKKDNPILLYIHGGPGSTEMFMSRKMDTEIVKDFVVVHW
                     DQRGAGKSYSPFIPSASYNREQYLSDTYELVSLLKKRFSRNKIYIMGHSWGSYLAAVT
                     AHRHPEDFCAFVGIGQMVNAVENESVSYDFALKRALIEENKEAFNDLLEIGKPPYKTL
                     GQMMTQRKWLTHFGGGMFHGEHSKDGFSYMGSFMSESPDYSLLDS"
BASE COUNT         1758 a         2009 c         1951 g         2082 t
ORIGIN      
        1 gttatgagcg cggatgacta cgatctggtg atgtctgcga gagacaaaga tggcagtatg
       61 tatgtgactg aatcgagtct gggcgtgttt ggctcgacag atcgcgctca attcattcat
      121 cgaatcaaaa acttcggagc tcccgaaaaa ctctcgtgga gcagacgtgc agttcagtct
      181 atgatcgcgc agctctccaa agagaaaatc gacatgttct tcgcggagtc ttcttcagtg
      241 cacactctgc gagtggatga agcgggcact ctgtacattg gagcgaatgt agatcacgat
      301 aacgcgttca tcgctgtttc gaaagaagga acgtccactt tgcttgcgag tatgagtcaa
      361 ttgagcaagg tcgccaatgg ctaccagact ggtggcactg cttctcgcct gtacgtttct
      421 ccgaaaggaa cagcctggct catggtccag ggaatcgaca agccaggtgg caaagattac
      481 ggaaccaatg ctttttatat gcgaagaaca gaagagggcg gttggcgcgc cactcctgtt
      541 ccagtgagat gtggcggcca gccatgcagc atgaaagtga aatacagcat cttctctcac
      601 ggcgtagcgg atgaaggggg agggtttgtg tttcatgatg ggaacggttt ctggcgcgct
      661 actccggatg gagatgtgaa gaatcttcta cagctcaaac tacctggagg agacgtgagg
      721 atcgtggggc cggtccttct ttccaacggg gacatctggt atgctctgag cactgtgtac
      781 agtgtgagtt cctacagcaa agtggaccag cagacaggct atgcggaggt tcggaatacc
      841 tggtttaccg tgggggatcg ctcccgattc atccgaatcc gagttcgcgg cggcaaagcg
      901 gagctgggcg aaatcagttc ggaggccatg tttgcggccc ttcgcaagaa aggggtccct
      961 ttgcgaccgg attctcaggt gatgaagacg gttcgattcg ttccggacta tgccacgggc
     1021 ggtttcattg cctttgacgc ccaccacagc gtgctttaca gcattttgcc gggagattga
     1081 aactatgtag gagttcctac cacataccga tcatactata tgcgccttct tttgatctta
     1141 ctgtttgcct ctcctctgct ggctcaggaa aatagccctg atttcccgga gtatctggct
     1201 cagggaacag agaacatccg agaccagcgc acgggggaag tagacataaa ccgggtagcg
     1261 caagcagctc ctacaaccac tcaaccggcg gcacctgtcc aaactggact gcccggattt
     1321 gcagagattc cgtggaaggc aacctactca gaagtgaaga ctcgtttgaa gaatctttcc
     1381 actgccgctg ttcaaacaga gcgcgtggaa atcctcatgg aggagaagaa caaaagcatt
     1441 ctggttcgtc gcaacgaagt actctatcgt tataactttt atcgcactcc tgtggaagtt
     1501 ctgcgcatcg caaatcacga ccttacagac gcggactatg aacagcaaga aggccagctc
     1561 ttccaggtgc gtgttacttt ctctctgatt gacgccgcga aaattcaaga cagaattgaa
     1621 aaatcgcacg gtgcgaagac caaatccacg gtagatccaa agaaaatgga aggggctgaa
     1681 atctgggaat tgccaggcgg tgtcatcctt gagtgggttg agccttatag aaaaatgaaa
     1741 ttctctcgca cggttgattt cctctcctct gaaatgacca aacagatcat gaaagaatac
     1801 gaggactact ttgatgcgcg cgaaaagtgg atcataaaca atattaaact ttactagtcc
     1861 gcgaatgacc tacgggcaag atcaccgcat caggtctgct gctccagatg cttgaggatt
     1921 tccttcactg cctctccatg atctatgtcc tcaaatgccg tataacgttc aaagcattct
     1981 tttgatttct tccagttctc aagccttgca tagagcaggc caaggttgaa aaaagtatct
     2041 gcatattccg gttcgattct ggctgcagtt tcaaaatgca tttgagcgag cggcagattg
     2101 cctgcgtcaa agtacaggtt tccaagatcg tagtgagatt caaagtgtcc gggctctgat
     2161 tccagtgcgc gtatgaattc agtcatggca cctggcacgt ctccgctttc aaacgcaaga
     2221 attccaagat tgcaatgagc gtccgcgcaa tgatcgtcct gttccagggc cttctggaag
     2281 aaggacgcgg cgcttgcatc tccgcgatcg agcagaatga gtccctcttc aaaaggagtg
     2341 cgcttctctg agagactcct aacaattcca gaagggcgag aggcgaacag atcgagttgg
     2401 cctctcatct cctcatcgat ttgtttaagt gtttttcctc tggttctttc ttcgcggatg
     2461 ctgcggaatc tggccagtgc acccaggcca aagcgaatct ctccttcttt gcccggaacg
     2521 gtttctaccg caccggcctc cgcccatttg cgcagaagct tttctgaaat accaaattgc
     2581 ttttgaacat cccgcagact ataggaagac tgcacccttt gcagggcggt ctggcgagtg
     2641 cctggaaact ggatgatgtt cgccacagcc gattcgcgcc ggccgggcta tgggatcaac
     2701 ccgctttcgt tttcctggtt tttactgcct ttgcggcctt ttctttgcct tccgctttgt
     2761 cctttgcctg ggccttttct tttttctcgc ctttggccgg aatcattcca ccgcgcatgg
     2821 ccgccgcttt ttcaatggat gctttcagag cagtcatgat gtccatggcc ggcttttcta
     2881 cttcttccac ctgaacgatt tcttttcctg ctacttttgc atcgatcatc tctttgagag
     2941 cgccgcgata gttgtctttg agttcgatgt cgctgagagg cctggccatg ctttccacca
     3001 gattgcgcgc cagcttcaac tgctctttgt ccgccggttc cactttgtct atctgtggaa
     3061 cttctgccat tttgcgtact tctgctggat aacggagcct gtatagcgcc agtccttttt
     3121 catgtggcga aagaagaacc acatcttctc tgtcacgtaa tacaactttg cctacgccca
     3181 ccttgcctgc gtctttgagt gcctggcaca aaagcgcata acttctggct gccacatctc
     3241 cgtccggtcc cgcgtagtag ggagattcaa acagcgtagg atgaacttcg tccacaccga
     3301 cgaatccttc tatctcaatg acctttgtac ttttcagttt tactttttcg aaatcagaat
     3361 cttcgaagac tacatactgg tctggctcat actcgtagcc ttttacgatt tcttctgtag
     3421 tcagggactt ctcgcatttc ttacatttct tgtcgtagcc cactctgcca ttgcattcgc
     3481 ggtgaagctg attgaagcgg atgctctccg atgtctcaat cgcgttgtag attcgcaccg
     3541 gaatcgtaac gagtgaaaaa cgtatgtgtc ccttccatat agatcgcata cactgattat
     3601 cgtcaggcac aatcgcacag gcaagcgttt aaaccagttc ggggctacag gtctggccgg
     3661 agccgaatga ataccggctc tcgtagcatt ccatcccgtg ttttggaagc gtattccact
     3721 tcgcagacga gactcgcttc tacccaggtg cctttttcga ccttaggcac tttcacggtt
     3781 ggcttttcta tttccatggt tgcgagccgt gcagagagat cgtttatgtc ttcatcgcta
     3841 aaaccggaac ccactttgcc gacataatgc agtccactct ccgttttctc tgccacatga
     3901 acggatccga gtttttcccg acctccttct ccttccgtgt acccaatgat gacgcagtcc
     3961 atggtagatc gcaccttgat cttgacccag gatgttgacc ttcttccgag cacgtaggga
     4021 gagtcaggcg atttggccat gattccttcg aggccatgtt cctttgctgc atcaaacagt
     4081 gcctggccgt ctggatagga ctggctgaat cggactgcac cccctgagcg aatcaaatcc
     4141 tgaacccatg ctcgtctgag atggagcgga tctttcaaag ttctttttcc gtccagagac
     4201 aatacgtcga acagatagat gaaggctgga aatttctgtt ccagtttctt gttcgctccg
     4261 ttctgtagtc gtcgtataac gttctgaaaa acaggccttc cgctcccgtc gaggcataca
     4321 atttctccgt cgagaactgc ggaggatgca tggtaggaat ccggcaccgc aatttcagga
     4381 aacgcagctg tgatatcgcg tccgtttctg cttctaattg tgatttgtcc atcttcaatg
     4441 aacacgagcg cgcgaattcc atcccacttt acttcaaaga tcatatcgcc tgacgggact
     4501 gtttccgttt gcgtggcaag cattggttca aaatcgaggc gtggctcttc gtcgagtcgt
     4561 tccatcagcc attctttgtc gcgcgtgtgg atcattctgt attcgccgtg cagtccctcc
     4621 gcatgagtgc gaaagtagaa tccatctttc ttttgttttg taatctcgta ctgaccgcga
     4681 gcgaagatcc acatttctcc tgcgccatac tgaccctttg gtatctttcc ttcaaaggta
     4741 agatactcaa tcggatgatt ctccgtatgc actgccagtc ttttgattcc ggggcgcgga
     4801 ggtaggcctc tgggcacggc ccacgaagtg agagccccat cttgctccag tcgaagatcg
     4861 tagtgaagat gcgatgcgtg atggcgatgg agcacgaaag actgtcctgt tgcggcagat
     4921 gaagctggtg cgggctcagg agttttgtca aaggatctct tcttcttgta cgtttccagc
     4981 tctgctgtca caggcgcgag cttcgcgcga gcgttatgcg gcactgctgt atgcgcgtac
     5041 attccttccc agggatctcc ctggcgaatg agatcgtatg catttcttaa gttatagtcc
     5101 agagggcttc tggctctttc caggtcttcc caggagagtg gcatggatac gggcgctccc
     5161 ggttttcctc ggaggctaaa aggagacacg atggtctgtc ccgagcgatt tctgtacacg
     5221 tctagaagta tgttttgagc gcgtctttct ttcttgattt cgagagtcac tcctttgttt
     5281 cgactcgcaa agtctatggc cgctgctttc gccgcttcaa atacaatgtc gtacgaatgg
     5341 ggaaggagag gcatgatgag atgcagtccc tttcctcctg ttgtttttac aaacacatga
     5401 taccccaggg attccaggta gggtcggagg ttcagcgcca gatctcgaac aatttcaaag
     5461 ttggaattct caggcgggtc caggtcaaac accatgtagt ctgcatcctg agaaacggaa
     5521 ggacgcattt gcatctggtg aaattcaagt gctgcgaggt tggcaagaaa cactacagcc
     5581 gcatcttctt ctgcaagcat gtaatcaatg tcgcctaact tcacagagtg aagccattca
     5641 ggtacccagt ccggcctgtt cttctggaag aattgttctc cttcaatgcc gtcagggaaa
     5701 cgcaccagag aaagaggtct gccacggaca tagcgcagca tgtaaggagc aatggttacg
     5761 tagtactgaa gtacttccgc ctttacaatt ccatcccctg gatacaggat tttgtccagg
     5821 ttagacagtt ctagcttgcg ttttccagca agaactgtct gcttctttgc catgtgtaaa
     5881 agcagaatgg tccgtgggat gcgcgcaaca agttttactt gaatgtctga gtgcgcgccc
     5941 tatgatcggt accgtgaagc gcttcgtttc tacttgcctg tgcttgctca cgaccctgtc
     6001 catggattgt gcacaaaagc caagcccgct gtcgattgac tgggggttga ttgcaatcgc
     6061 cgcctccata gtgaagattc cgcgcttcgt atttgtcaca agcaacacag gtaacgttta
     6121 cgtttactcg attaatccgt tcgtttgctc gctcaatccc ttgtccccaa tgtgggcgcc
     6181 cgcctccccc gggattcctg ccattgattc tacaggacag tttctctttg tgcccgggag
     6241 cgtggccaat cagatcgcaa gctaccgtat caacactacc accggggtcc tcacccaggt
     6301 gggtttcgtc tctactacat cacccacagc gcttgcctat gatcccgcag taggtcaggt
     6361 tctcagtgta tcaaccggtt cctccatgat aagccgtttc gcgctgatgc ctgacggcag
     6421 ccttacctct gcaggcagcg ttgcaatgcc tgctccggca agcggcaaca ttgcaataga
     6481 tactggatct agaatcgctt ttgtgccgca cgggatatcg ttgttttctg ccgcatcgct
     6541 cgcggcagga gttgcgtcgt cctccagtgc gcctgcggtt gccacgagtg cagcgcttaa
     6601 cggcggttat ttgtacgtag gcggcagcac taccattctc acgtatcgtt tgaatggtac
     6661 gaatgcaccc ggctttgtga gtacgactcc ttctgcaggc actcttactc ccctggccgc
     6721 agactctcag ggagcgaatc tgtacggagc ggacgcaacg ggcgttttga atcgctacgc
     6781 gattcaaaac ggtggaacgc tcctactcaa ttcgcagtcg ctatttggtc caagcgcgaa
     6841 ctccattgca gtcgatccca ccggaacatg tgttgtcatg tctggcggtt caaatgttag
     6901 agcctttcga acttcttctg caggcttaac gctcttcgct gccgggttcg tctcaaatcc
     6961 aaacgcggtt ctcatcgcat cctattgact ctctacgttc aggattgaaa atgaaaacag
     7021 taaagaagat ctttcttgga attgcggcag tgcttgccgt gcttctggtg tacgtgtttg
     7081 tctccaaggc aatcacggat cgcaaaatgg aaagagagac cgcaatgccg gaaggcggaa
     7141 tcagttctct tgaatctgta acgataggcg gcatttctca gtggattctg atccgtggag
     7201 aaaaaaagga caaccccatt ctgctataca ttcacggcgg ccctggatcg acggaaatgt
     7261 tcatgtctcg caagatggat acggagattg ttaaagattt tgtagtagtt cactgggatc
     7321 agaggggcgc aggcaaatct tattctcctt tcattccttc tgcttcctac aacagggaac
     7381 aatatttgag cgatacatat gaactggtat ctctgctcaa gaagcgattt tcccgcaata
     7441 agatctacat catgggccat tcctggggat cgtacctggc ggccgtgacg gcgcacaggc
     7501 atccagaaga tttttgcgca tttgttggaa tcggtcagat ggtcaatgca gtggaaaacg
     7561 aaagtgtgtc ttacgacttc gcattgaaaa gagcgctcat agaggaaaac aaagaagcat
     7621 tcaacgatct cctcgaaatt ggaaagcctc cctacaagac tctgggccag atgatgaccc
     7681 agcgcaagtg gctgactcac tttggcggcg gtatgtttca tggggagcac agcaaagacg
     7741 gattttctta catgggaagt ttcatgtctg aatcgccgga ctactctctt ctggattcgg
//
LOCUS       DAORFF010000116         7796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4633_length_7796_cov_4.196228, whole genome shotgun sequence.
ACCESSION   DAORFF010000116 DAORFF010000000
VERSION     DAORFF010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7796
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4633_length_7796_cov_4.196228"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..921)
                     /locus_tag="PKK76_06360"
     CDS             complement(121..921)
                     /locus_tag="PKK76_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68127.1"
                     /translation="MKFESSANRNEKMHKPFDLRLDLNAKTMSFDGKTIPLEKFNFQS
                     ASYHTMGDYGFESEMLFVSGKGKNREFLIKTRSAPEHEDEFLYFTSSRNEIHPIQATH
                     WRYVLDPKAGGETHFAQLHADVAIPGQGPAQIKLDVVRTPATDVGFWLLRKNKSPTRF
                     WQTNDSNTRRFRFCANSSASDEAGCSVLTIANGTGNPACSGVEIGTIVVMLNQGKAQI
                     LSDDQKGHLLDEDCKVVYSLEYREEINAVSIKGSEGPAKVFVEKQPDP"
     gene            complement(1194..1994)
                     /locus_tag="PKK76_06365"
     CDS             complement(1194..1994)
                     /locus_tag="PKK76_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNL68128.1"
                     /translation="MKIPMYQVDAFTQKVFAGNPAAVCPLETWLPESTMQAIAAENNL
                     AETAFFASAANDFQIRWFTPEAEVALCGHATLASAVVARHLNLFQDRICFHSKSGPLF
                     VDRKDDLYTLNFPLRTIGPEIQVSPAIAECLGTPPVRLFRSEPNYLAVFSSEEEVRRL
                     NPDFRKMKEIAGHGIIATARGTEFDFVSRFFAPAIGVDEDPVTGSAHCTLIPYWSAVL
                     NKTRMVAYQASRRGGVLNCELKDERALIGGYGALYSEGFIFISEAGTR"
     gene            2085..2789
                     /locus_tag="PKK76_06370"
     CDS             2085..2789
                     /locus_tag="PKK76_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNL68129.1"
                     /translation="MWFDYSAHPGKKPWASNILNFIGVLFGILFFKVKIEIDPDAQKN
                     GPVLILSKHSAEFDIPLGYHAMKRAFGRHAWCIMKASLVRPRYLGFFWKIGGIPLERE
                     DPQQSKNYLVYARKVLYEKDITGLKDGDGNLMVLFPEQTTYWRQMGEGKLAGFRFLAG
                     KPARPLACNSVGFRYKPGFPRTSVVIRFGPTRYFTADMDHGLFLHERMKEIAELSALE
                     YSFPAPRSRKAEAVVG"
     gene            complement(2790..3464)
                     /locus_tag="PKK76_06375"
     CDS             complement(2790..3464)
                     /locus_tag="PKK76_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNL68130.1"
                     /translation="MLASISEFCFFNAMNILVIGASKGTGAICVQNALERGHSVTAFS
                     RSPEKLALNHSRLRLYPGSFHDAANVQAAVPGHDAVIITASASSMQAFKENPNYFSNG
                     TAIVIDAMKKHRVPRLVVLSALGVGESGKLMPWPLRKIVVSWLLKLPFADHERQETMV
                     QSSGLDWVIARPGRLTDGPAKKQYQKKSQIESIPTSISRADVADFLVEACEPGEWVRK
                     AVQIGG"
     gene            3557..4051
                     /locus_tag="PKK76_06380"
     CDS             3557..4051
                     /locus_tag="PKK76_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNL68131.1"
                     /translation="MPTHYKGSDAEVRALDAFIKLARASSSLASRLEGSMHLEGLTEP
                     QLGVMETIYHLGPMCQKELGAKQLSTGGNITLIVDNLEKRGLVERVRNTEDRRYITVH
                     LTEEGKKLMKRVFPRHAKAIAEEFAVLSPDEQTMLSSLCKKLGLGKPEYEEALTKVRR
                     SSDG"
     gene            complement(4020..4712)
                     /locus_tag="PKK76_06385"
     CDS             complement(4020..4712)
                     /locus_tag="PKK76_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNL68132.1"
                     /translation="MNLHCILLSGGTGERMAAGKPKQFIELLGIPVLRWSALTLSNFS
                     KRRLTVVAPANLLAETRSILEGIDSVVVPGGSTRHDSTLCGIRSIAKEIHLQSDIILI
                     HDAARPVIRHAELSRLCDLFKDETILCSSLVGPIVETIVQSRDGKTSDGIADRDQLRA
                     VKTPQALRANALRALLEVEGNFTDLLSWAHATGLKAGLALAEETNVKLTSQGDLDLML
                     SLLNRLKIDGPS"
     gene            complement(4709..5386)
                     /locus_tag="PKK76_06390"
     CDS             complement(4709..5386)
                     /locus_tag="PKK76_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNL68133.1"
                     /translation="MKQSELSQKLQIKSGMRIGTMNAPDMFARKLAPVPEGSELSPVA
                     PYDHLTVFVNRKAELDRFALGAMKSMRPGGILWFAYLKKASGAKSDVTRDDGWDALTR
                     AGWGPVSLIAIDGTWSALRFRPLTKEEQEDRANRKVPLDEGIEKKPSKPKPSVARKPP
                     AVPADLTAALAKNKKAKQFFESLAPSHAKEYIVWITEAKKAATRETRIAKTIEKLLQK
                     KKNPTIK"
     gene            complement(5427..6578)
                     /locus_tag="PKK76_06395"
     CDS             complement(5427..6578)
                     /locus_tag="PKK76_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004780672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNL68134.1"
                     /translation="MRIEIYKKGRAVKQNYDWKEELRSRISSADELLEFMPDLPESPL
                     FRRLYASVEDFRKVAEAMAGEFRFSVTPYYLSLANLENARCPILLQILPRREELEDQV
                     FQFPDPLAEDSHHPVDGLTHRYPDRVLWYLSHNCAVYCRFCMRKRKVSRSNSAPDRSS
                     YSAALDYIRNHKEIKEVILSGGDPLSHSDQTIQFLLTELRTISHLSSLRIHSRMPVTL
                     PSRITDQLTDVMRQAFPVTLVTHFNHPVEITEEATSAVRRLRMNGVTVLNQTVLMRDI
                     NDSTQTIEELMLGLLRIGIKPYYLHQCDEVHGVSHFRANIETGLQILRDLQGRNPGIA
                     IPRYVIDLPGGGGKVPLENQYLRRRTDDGLEFENWSGLKFTTVQDREDK"
     gene            6577..7140
                     /gene="efp"
                     /locus_tag="PKK76_06400"
     CDS             6577..7140
                     /gene="efp"
                     /locus_tag="PKK76_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNL68135.1"
                     /translation="MMDPIDLRKGQVIKVDNDLYTVTFSQFVNPGKGSAFVRTKLRNL
                     KRGTVVERTFKAVEKIDDVDLEKRYMTFLYKDGDHLVFMDKDDYEQLSIPMVLVEDIV
                     PFMKEEMPFEITFYEGNPVGVTPPNFAELEVTYAEDAIKGDTVGTSRKKVTVETGGEV
                     LVPLYVKQGDRIKIDLREISFVERVSK"
     gene            7151..>7796
                     /locus_tag="PKK76_06405"
     CDS             7151..>7796
                     /locus_tag="PKK76_06405"
                     /inference="COORDINATES: protein motif:HMM:NF027399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNL68136.1"
                     /translation="MSKIISQAKAGQGRLFVQFGGQGAPWYKELAKYYKEPSMKKFFD
                     VVLRAIEEERPHVDGTVGLPHGLDARAWLEDESKIPSEDYLACAAVSIPMIQVTQLAH
                     FENTVNKGISRKDLVAATEATSGHSQGLIPASLVALDLDGDAYYAAAAKYVKYLLYLG
                     VRAQEVYPFFAPTADEIARSEKAGNKAPAPMMAVVGDSHETIQAMVDATKKELPA"
BASE COUNT         1906 a         1878 c         1992 g         2020 t
ORIGIN      
        1 gaagtcgtga tccgcgtact actgtgggtt ctgcaagcag tggaccgcat agtgccaaaa
       61 gcaagaatgt tgttttgatc catttcattt tgaatgagtt cgtttctgtt tcttctggga
      121 tcagggatct ggctgttttt ccacgaatac ctttgccggc ccttcagatc ctttgatgga
      181 gacggcgttt atttcttccc gatattccaa cgagtaaacg accttgcaat cttcgtcaag
      241 cagatgtccc ttctgatcgt ctgagaggat ctgcgctttc ccctggttca gcattacaac
      301 gatggttcca atttcaacac cggagcaggc agggttcccc gttccgtttg cgatcgtgag
      361 aacagaacag ccagcttcgt cactggcgga ggaattcgcg cagaaccgaa atctgcgagt
      421 attcgaatca ttcgtttgcc agaatcgcgt tggagatttg tttttgcgca agagccagaa
      481 gcccacatcc gttgctggag tgcgcacaac atcgagtttg atttgggccg ggccctgtcc
      541 tggtatggca acgtctgcgt gtagctgcgc gaagtgagtt tccccgccgg cttttggatc
      601 tagaacgtac cgccagtgag tcgcctgaat gggatggatt tcatttctgg aagatgtgaa
      661 atatagaaat tcgtcttcgt gttccggtgc gctccgtgtc ttgatcaaga attcgcgatt
      721 cttccccttg ccggatacaa agagcatttc agattcaaat ccatagtctc ccatcgtatg
      781 gtaggacgcg gattgaaaat tgaatttttc aagagggata gttttgccat caaaggacat
      841 ggttttcgca ttcagatcga ggcgaagatc aaagggctta tgcatttttt cgtttcgatt
      901 tgcagaagat tcgaatttca tagcaacctg ccatacattc gcttcaattt gtttgatctt
      961 ttcgcccttg taggattgag ctgaaaagac gtaaggtttt ggtttattgg gcaggcatga
     1021 cgtcgcacaa atcagagtaa tggcggttaa cgttcctttt cttgaaacag aaagataaaa
     1081 ttcattctcg gtggatggat cctccccatg tatgctgtga aaaataccgg ccggaacgtt
     1141 ctgtgcgtgc gaaactgttg cgcatactag aagtgtcagg caaacaatcc tcttcatcga
     1201 gtccccgctt cacttatgaa aatgaatcct tctgaataga gtgcaccgta tccgccaatc
     1261 agagctcgct cgtctttcaa ttcgcagttc aatactccgc ctcttctgga tgcttgatac
     1321 gccaccattc tggtcttgtt tagaaccgca gaccagtaag ggatgagcgt gcagtgtgca
     1381 gagccggtca caggatcttc atctacgcca atggcaggtg caaagaagcg tgatacaaaa
     1441 tcaaattcag tgcctctggc tgttgcaatg attccatgtc ccgctatctc tttcattttt
     1501 ctaaagtcag gatttaaacg ccgcacctct tcttcagacg aaaagactgc aagatagttt
     1561 ggttctgatc tgaataatct gaccggaggg gttccaaggc actctgcgat ggcgggagaa
     1621 acttgaatct ccgggccaat cgtgcgcagt ggaaaattca aagtgtagag atcatctttt
     1681 cgatccacga agagaggacc gcttttggag tgaaaacaga ttctgtcctg gaacaggttc
     1741 agatgtcggg ctacaacggc agatgcaagt gtggcgtgtc cacagagagc cacctccgcc
     1801 tccggtgtga accagcggat ttgaaaatca ttcgcagcgg aggcgaagaa cgcagtttcg
     1861 gcgagattgt tctctgctgc aattgcctgc atcgtgcttt ccggaagcca ggtttccagt
     1921 gggcagactg ctgcgggatt gcctgcgaat accttctgtg taaacgcatc tacctgatac
     1981 attgggattt tcaccgatat ctccagcccc acacattcgt gcattgtagg gatgctcaat
     2041 gatttttcgt ttaccgcgct gcgcatttcg cgcgatcgtt cacgatgtgg ttcgattaca
     2101 gtgcccaccc cggaaagaaa ccctgggctt ccaacatttt gaatttcatt ggagttctgt
     2161 ttggaattct cttcttcaag gtgaagatcg aaatcgatcc tgacgcacag aagaacggac
     2221 ctgtactcat tctatccaag cacagcgctg aatttgacat tccactcgga taccatgcga
     2281 tgaagcgcgc tttcgggcga catgcctggt gcatcatgaa ggcgagcctg gttcgtcctc
     2341 gttatctggg attcttctgg aagataggcg gaattcccct ggagagagaa gatcctcagc
     2401 agagtaagaa ctatctcgtt tatgcgcgca aagttctcta tgaaaaggac atcactggat
     2461 tgaaagacgg agatggaaac ttgatggttt tatttccgga gcaaaccacc tactggcgac
     2521 agatgggaga aggaaagctc gcgggcttta gattccttgc tggcaaacca gccagacctc
     2581 ttgcctgcaa ttcagttgga tttcgttaca aaccaggatt tcctagaacg agcgttgtga
     2641 ttcgctttgg gccaacgcgt tacttcaccg cagacatgga tcacggtctc ttcttgcacg
     2701 agcgcatgaa agagatcgca gagttgtccg cacttgaata ttcttttccc gcaccgcgtt
     2761 cgcgaaaagc ggaagctgtc gtgggataac tagccgccta tctgtactgc ttttcgcacc
     2821 cattcgccgg gttcgcaagc ttccaccagg aaatcggcaa catctgcccg cgaaatggaa
     2881 gttgggatcg attctatttg agatttcttc tggtattgtt tttttgccgg cccatctgtg
     2941 agacgtcctg gtctcgcaat cacccaatcg agtccactgg attgaaccat tgtttcctgt
     3001 ctttcgtgat ctgcgaacgg aagcttgaga agccaggata ctacgatctt tcgtagtggc
     3061 catggcatga gcttcccgct ctcacccacg cccagggcag aaagaacgac caggcgaggc
     3121 accctatgtt tcttcatcgc atcgataaca attgcagttc catttgagaa gtagttcgga
     3181 ttttctttaa acgcctgcat cgaggatgca gaagctgtga tgataacagc gtcatgcccg
     3241 ggcactgcgg cttgaacgtt tgccgcatca tgaaaactgc ccgggtaaag gcgaagacga
     3301 gagtggttga gagcaagttt ttcaggagag cgagagaagg cagtaactga atgaccgcgt
     3361 tcgagagcgt tttgcacgca gatggctcca gtgcctttgg aagcgccaat gacgagaatg
     3421 ttcatcgcat taaagaagca gaattctgag attgaggcga gcaattcgct tcgttcagtc
     3481 gctacatttc tcgtttcgag tcggaatgac cttgacattt tgactttttg tagcatctta
     3541 agatcaagag cgcgctatgc caacgcatta caaaggatca gacgcagaag tcagagcact
     3601 cgacgcgttc atcaaactgg cgcgtgcatc ttcgtctctt gcttcaaggc tcgaaggttc
     3661 catgcacctt gaaggactca cggagccgca actcggtgta atggaaacaa tctaccatct
     3721 gggtcctatg tgccagaaag aattgggagc aaagcagctt tccactggcg ggaacatcac
     3781 tctgattgtg gacaacctgg agaagcgcgg cctcgtggaa agagttcgaa acacagagga
     3841 tcgtcgctac attaccgttc acctcacaga agaagggaag aaactcatga agagagtatt
     3901 ccctcgacac gcaaaagcaa tcgcagaaga atttgcggtt ctcagtccgg atgaacagac
     3961 catgctttcc tctctctgca agaaactggg actgggaaaa ccggagtacg aggaagctct
     4021 tacgaaggtc cgtcgatctt cagacggttg agcaagctca acatcaaatc cagatcccct
     4081 tgagaagtta gtttcacatt tgtttcctct gccagagcca gacctgcctt caatcccgtg
     4141 gcgtgcgccc agctcaaaag gtccgtaaaa tttccttcca cttccagaag tgcgcgaaga
     4201 gcatttgcgc gcaatgcctg cggagttttt accgcgcgga gttgatctct gtccgcaatt
     4261 ccatcagatg ttttcccatc cctcgattga acgatcgttt ccacgattgg acccacaagg
     4321 gaagagcata ggattgtttc gtctttgaag agatcacaga ggcgcgaaag ttcagcgtgt
     4381 ctgatgactg gtcttgcggc atcgtggatg agaatgatat cgctctgaag atggatttcc
     4441 tttgcaatgg atcgaatgcc gcataacgta gaatcgtgcc gagtggatcc acctggaact
     4501 actacgctgt ctatcccttc cagtatggat ctggtttctg caaggaggtt ggccggagct
     4561 accaccgtca ggcggcgttt tgaaaagttt gaaagtgtca gagcgctcca gcgcagaaca
     4621 ggaataccca ataattcgat gaattgtttg ggttttccgg ctgccattcg ctctccggtt
     4681 cctcccgaga gcaggatgca atgtagattc attttatggt tgggttcttc tttttttgga
     4741 gcaacttttc gatggttttg gcgattctag tttcgcgagt ggcagccttc tttgcttctg
     4801 tgatccagac aatgtactct ttcgcgtgag atggcgccag actttcaaag aattgtttag
     4861 cttttttatt tttggcaagg gccgctgtta agtcggccgg cactgcggga ggttttcgtg
     4921 cgacgctcgg tttaggtttg gatggctttt tttctattcc ttcgtccaga ggaaccttgc
     4981 ggttggcacg atcttcttgt tcttccttag tgaggggccg gaagcgaaga gcagaccagg
     5041 tgccgtcaat ggcaatgaga gataccgggc cccatcctgc ccgcgtaaga gcgtcccatc
     5101 catcgtctcg cgttacgtcc gattttgcgc ctgatgcttt tttcaaatag gcaaaccaga
     5161 gaattccacc cgggcgcata gatttcatcg caccaagagc gaaacgatcc agttccgctt
     5221 tgcgattcac aaatacagtc agatgatcat agggtgcaac cggcgaaagt tccgatccct
     5281 ctggaacggg cgcgagcttt ctggcaaaca tatccggcgc attcatggtc ccaatccgca
     5341 tgccagactt gatctgcagt ttctgtgata gttctgactg cttcacgtac tagaagtcga
     5401 ccgggcccgg gcacacggcg agcattttat ttgtcttcgc gatcctggac tgtagtgaat
     5461 ttcaaaccgc tccagttctc aaattccagg ccatcatccg tcctgcgcct gaggtattga
     5521 ttctccaggg gcaccttccc tccgccgcca ggaagatcaa tgacgtagcg cggaattgca
     5581 atgcccgggt ttcttccctg aagatcgcgc aatatttgca aaccagtttc tatgttcgcg
     5641 cgaaagtgcg aaaccccgtg cacttcatcg cactgatgca gatagtaggg ctttatgcct
     5701 atacggagca agcctagcat caattcttct attgtctgtg tggagtcgtt gatatcgcgc
     5761 atgaggactg tctggttcaa tacggtcaca ccgttcattc tcaatcttcg cactgccgag
     5821 gtagcttcct ccgtaatttc aactggatga ttgaaatgag tcaccagagt aacagggaag
     5881 gcctgtcgca taacgtctgt aagttgatct gtaatccgag acggcagagt gaccggcatt
     5941 ctactgtgga tgcggaggct ggagagatgg ctgattgtgc gaagctccgt aagcaagaac
     6001 tgaatggttt gatccgaatg cgaaagtgga tctccgccgg acaggatcac ttctttgatt
     6061 tctttgtgat tgcgaatgta gtcaagtgcc gcagaatacg aagaccgatc cggtgcgctg
     6121 tttgacctgg atacttttcg ttttcgcatg cagaatcgac agtagactgc gcagttgtgc
     6181 gaaagatacc agagcacacg atccgggtac cgatgagtga ggccgtccac gggatgatga
     6241 gaatcttctg ccagaggatc cgggaactgg aacacctggt cttcgagctc ttcccggcga
     6301 ggaaggattt gaaggaggat ggggcatctg gcattctcca gattggccag ggacagatag
     6361 taaggagtta cagagaatcg aaactctccc gccatggctt ccgccacttt tcggaaatct
     6421 tcaacagatg catacaaacg gcgaaagagc ggcgattctg gcaaatccgg catgaattcg
     6481 aggagttcat ccgccgaact gatgcgagat ctcagctctt ccttccaatc gtaattctgt
     6541 ttgactgccc tgcctttttt ataaatctcg attcgcatga tggatccaat cgacctgcgt
     6601 aaaggccagg tcatcaaagt agacaacgac ctctacactg ttaccttctc tcaattcgtc
     6661 aaccctggaa aaggcagcgc atttgtccgc acaaagctca gaaacctgaa gcgtgggact
     6721 gtagttgagc gtaccttcaa agcggttgaa aagatcgacg atgttgatct cgaaaagcgc
     6781 tacatgactt ttctatataa agacggtgat caccttgtgt tcatggacaa agatgactat
     6841 gagcaactaa gcattccaat ggtgctcgtg gaagacatcg ttcctttcat gaaggaagag
     6901 atgccttttg aaatcacttt ctacgaaggc aatccggttg gtgtcacgcc ccctaacttt
     6961 gcagaactag aagttacgta tgcggaagac gcgatcaaag gggacacggt tggaacttca
     7021 cgaaagaaag taaccgtgga gaccggcggg gaagtacttg tgccattgta tgtaaaacag
     7081 ggtgatcgca tcaagatcga cctgcgcgaa atttccttcg tggaacgcgt aagcaagtaa
     7141 ggataggtat atgtcaaaga tcatttctca ggccaaagcc ggacagggcc ggctgtttgt
     7201 tcagtttggc ggccagggtg cgccctggta caaggaactt gcaaaatatt ataaagaacc
     7261 ttctatgaag aaattcttcg atgttgtgct tcgcgcaatt gaagaagagc gtcctcatgt
     7321 ggatgggaca gtgggtctgc cgcatggtct ggacgcacgt gcctggctcg aagatgaatc
     7381 aaagattcca tctgaggatt atcttgcgtg cgctgctgtg tcgattccca tgattcaggt
     7441 tacacaactt gctcattttg aaaacacagt gaacaaggga atctctcgca aggacctcgt
     7501 tgcagccaca gaagcaacca gcggtcacag ccagggcctc attcctgcgt ctctggttgc
     7561 actggatctc gatggagatg cttactacgc cgccgcggca aaatatgtga aatacttact
     7621 atatctgggt gtgcgcgcgc aggaagtata tccattcttc gcgcccacag cagatgagat
     7681 cgcaagatct gaaaaagcag gcaacaaagc tccagcgccc atgatggcgg ttgtaggaga
     7741 ttcgcacgag accattcagg caatggtaga tgcaaccaaa aaagagctgc cagcgg
//
LOCUS       DAORFF010000117         7743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4678_length_7743_cov_3.338970, whole genome shotgun sequence.
ACCESSION   DAORFF010000117 DAORFF010000000
VERSION     DAORFF010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4678_length_7743_cov_3.338970"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PKK76_06410"
     CDS             <1..840
                     /locus_tag="PKK76_06410"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNL68137.1"
                     /translation="SCNSGAASKDSVTDADILSGLSDAVATAASAVLRVFNDKSFSVS
                     SKGDAGPLTTADLESNRILESHLRRLLPDAGWLSEESTDDDDRLSCREVWIVDPIDGT
                     KEFVAGQKDFSVSVGLSRNGTPVLGAVAMPAEDILISGGMGVMKNGVRASISRRRELI
                     GSTLLVSQTEFQRGTFAELQNDFEIRPTGSIARKLAMLAGGEGDLVVSLYPKNDWDIC
                     GGTALVLAHQETMVTDLENRKARHYNQKDPKSFGLVAGNTDLVLQFHEYFLRRGLKLA
                     RKY"
     gene            911..1426
                     /locus_tag="PKK76_06415"
     CDS             911..1426
                     /locus_tag="PKK76_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68138.1"
                     /translation="MQELTFDSFQDSKTISVSGQNVSAYPSGHGCVYFEWAFGNRSEK
                     KWESYSVGFQSEVQLNLKTENGKMTVSRADIRLYLNPAVEKYYTKESDAPEAVRAWIR
                     EHGSVSVAEYRLETGKKYYAVLHVDHYYLPRPGGPPSSHSKKVLWISDEPIKDGKPSV
                     EITPAYRGWTY"
     gene            1461..1676
                     /locus_tag="PKK76_06420"
     CDS             1461..1676
                     /locus_tag="PKK76_06420"
                     /inference="COORDINATES: protein motif:HMM:NF028518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNL68139.1"
                     /translation="MPEVIQDELFEGALKTGRPISLYLKTGVPVRGKVRAHDGFTVLL
                     EQEGRSILVYKHSITSVLGARPGQRKK"
     gene            1673..2785
                     /locus_tag="PKK76_06425"
     CDS             1673..2785
                     /locus_tag="PKK76_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNL68140.1"
                     /translation="MKHLRIGVDARPLSSRVSGVARVIASVLRELSDDHEFFLYSAQN
                     PHADYAEILAHKKIHWMQGQGLLAWKQGLWFNSALPATLGKENLDLFWGSQQVLPPFL
                     PRIPAVLTFYDLVLLYFPGAMRRIARIQQRAFLNYSVKRASRILCISDQSRLDMIREL
                     GVPEEKTAVSLLGVDPPAKRKKSEQRKPPVNGPFILSVSTLEPRKNYGMLLDAYGQYA
                     SHAMARAKPYPLVLVGRRGWESADFYQKLKGMEDTGLVHVLDSESDESISDLYEQCAF
                     FVLPSLYEGFGLSLLEAMARGKHCIASDLGCFHEIGGSQVTYLGSKDVHAWRDALADA
                     VSKHRSGKLRPTKIKLSDWSWRNTALVHADAFAAVV"
     gene            complement(2755..3144)
                     /locus_tag="PKK76_06430"
     CDS             complement(2755..3144)
                     /locus_tag="PKK76_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNL68141.1"
                     /translation="MLKKKESPSITEGGPISTILGKETSFSGVMEFKKPLQINGHFEG
                     ELITDGVLVVGEGATVKANIRAGIVIVAGEVTGNIEARQKLEMLTSGKVYGNVKTAKL
                     QIADGVVFDGNCEMITDGQTTAAKASA"
     gene            complement(3146..3367)
                     /locus_tag="PKK76_06435"
     CDS             complement(3146..3367)
                     /locus_tag="PKK76_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL68142.1"
                     /translation="MLTKQQEEVVALYNQGLGLYKARKFKEARETFLKGLQILPEDGP
                     SKLYVERCDAYIKDPPGEDWDGVYVMKTK"
     gene            complement(3402..4934)
                     /locus_tag="PKK76_06440"
     CDS             complement(3402..4934)
                     /locus_tag="PKK76_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNL68143.1"
                     /translation="MSSGKRAEDLHVGLMEGAATKEGTARYFLDLKTQFAEYRDHSWF
                     RFQTPSHLALSRIGFGCYRVSAGARANVDALREAILTGVNVIDTAANYSDGSSEQMVG
                     DVIRQLVEAKRIERNQVAVVTKIGYIQGRNLGLLRTAPPPEVVKVRDDIWHCIHPDYI
                     KSQLQLARYRLGLRTIDFLLLHNPEYFLIDAQTRGVLADEARAQYVDRLRRAFEMMEE
                     MANRGIIQYYGISSNTLAHSSSSYTATRLDEVLSVAGPRFAAVQFPANLIENDFRYNR
                     SAGGGTVTSVALAHGLWTLGNRPFNANGPRGLIRLARLVDAPPDGGDSGIAEFQKMQG
                     RLMELEAQLHDLFGSAFQFDKTAPAFSDILERYRDRFTTTDQLRASVEQITHLFSPTA
                     RRIQTAVQTKNQEIGFNQYLKVVNAVIHYWERYVEILVHQRMEGLENLLGEHPALQGK
                     PLALQVLLILLSGDVPATALVGMRKIPYVRQLVQAFAEPSPPEREMLSLVGRALDLIE
                     KF"
     gene            complement(4931..5638)
                     /locus_tag="PKK76_06445"
     CDS             complement(4931..5638)
                     /locus_tag="PKK76_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68144.1"
                     /translation="MSGMLEFYRGNSETPSGSLLFYCRVRGPNPFSPDAKLLVCHVVV
                     SYLGVRNNNFPVVVFPPSALTSEEEFFELLDLDEDSDVVRLEDFEIPEGKPEADYMSE
                     RLERFNDRVYEYVEIYRKQLDRKRKKHGQQRLPLFPEQPLLIQAPDMDDAHAIATIEQ
                     AITDGLQSKESIQAAVEFLKHQHPQLDMINLESAVNQEDLKLSALYLDKFKAILEEKY
                     EDAARIQVQIRNFNPAG"
     gene            complement(5675..6271)
                     /locus_tag="PKK76_06450"
     CDS             complement(5675..6271)
                     /locus_tag="PKK76_06450"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNL68145.1"
                     /translation="MRLRIVEDVRSIFRNDPAARGLEFLLYPCLHAIFWHRLVCHPLY
                     NVGLRFFARFFSQMSRFFTGIEIHPGAKIDGGFFIDHGMGVVIGETAIIGRDCMMFHG
                     VTLGGTGKHKGRRHPIIGNDVLIGTHATLLGPIHVGDHARIGAETVIINRDVPANCTV
                     VGTPGKIVKQGNKKLKRPESLPISQYRVEELEAEKNGT"
     gene            complement(6261..6794)
                     /gene="dcd"
                     /locus_tag="PKK76_06455"
     CDS             complement(6261..6794)
                     /gene="dcd"
                     /locus_tag="PKK76_06455"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002108085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNL68146.1"
                     /translation="MILTGPEIVRRMGTEIKIEPFDASLINPNSYNLRLNDELMIYKE
                     RALDMKKPAQTEMIKIPPEGIVIEPGTLYLGRTVEYTETFNLVPMLEGRSSIGRLGLF
                     VHVTAGFGDVGFRGFWTLEISAIQPIRIYPFVPICQIFYHTIEGEVLEYKSGKYQNNT
                     GIQPSLLFKELEKNNAT"
     gene            complement(6831..7604)
                     /locus_tag="PKK76_06460"
     CDS             complement(6831..7604)
                     /locus_tag="PKK76_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNL68147.1"
                     /translation="MSLLIEERIEHTIRLRLNRPDALNAMSRELLALLETRIQALSVD
                     PAIRALVLTGSGRSFCTGADLKERASMSPPEVRDFLIRVGHIFQAIESFPCPVICGIN
                     GFALGGGLELALACDFRIAQTDALLGLTETSLGIIPGAGGTQRLPRLIGVSRAKALIF
                     TARKVGASEALSIGLVDSVVNAEKLDEACVELASSISKNAPIAVRSAKFAINAGANVD
                     LSTGLAIERNAYEITIPTKDRIEALHAFREKRAARFTGE"
BASE COUNT         1729 a         1937 c         2037 g         2040 t
ORIGIN      
        1 tcctgcaact ccggcgcagc cagcaaagac tccgtgactg acgcggacat tttgtccggc
       61 ctatctgacg cggtggcaac cgccgcgtcc gccgttctgc gagtattcaa cgacaaatca
      121 ttctccgttt ccagcaaagg ggacgcgggg cctctcacca ctgctgacct cgaatccaat
      181 cgcattttag aaagtcatct tagacgcctt ctgccggatg cgggctggct ttctgaagag
      241 tccacagacg atgatgatcg actttcttgt cgcgaagtct ggattgtaga tccaatcgac
      301 ggaacaaaag agtttgttgc gggtcagaag gatttctcag tttccgtggg actatctcgc
      361 aatggaactc cagtgctagg cgccgtggcc atgcctgcag aagatattct gatttcaggc
      421 ggcatgggag tcatgaagaa cggagtgagg gccagcatct ctcgccgccg ggagttgatt
      481 ggatccacat tgcttgtgag ccagactgaa tttcagcgag ggacatttgc agaactgcag
      541 aacgatttcg aaatcagacc caccggttcc attgcgcgaa agctcgctat gcttgcgggt
      601 ggagaaggcg atcttgtagt ttcattgtat cccaaaaacg attgggatat ttgtggtggc
      661 actgcccttg ttctcgcgca tcaggaaacc atggttacag atttagagaa tcgcaaagct
      721 cgccattaca atcagaaaga tccaaaatcg tttggccttg ttgccggaaa tacggatctt
      781 gttctgcagt ttcacgaata cttcttgaga cggggattga agcttgcgcg taagtattag
      841 cgttgcctgc gtttgttttt ttctatttcc agaatgtgcc cgccccgtgc gcgagcccca
      901 ggagagttcg ttgcaagaat tgacatttga ttcgttccag gatagcaaaa cgatttctgt
      961 ttcgggacag aatgtatctg cttatccgtc cggccacggc tgtgtctact ttgagtgggc
     1021 tttcggaaat cgctctgaga aaaaatggga atcctatagc gtgggttttc agagcgaagt
     1081 gcagctgaat ttgaaaacag aaaacggaaa gatgactgtt tcgcgcgcgg acatccggct
     1141 ttatctgaac ccggccgtgg agaaatatta cacaaaggaa tcggacgcac cggaagcggt
     1201 cagggcctgg attcgagagc atggttctgt aagtgtggca gaatatagac tggagacggg
     1261 caagaagtat tacgctgttt tgcatgtgga tcactattat cttcccaggc cgggtggccc
     1321 tccttcatcg cacagtaaga aggtgctctg gatttctgac gaaccaatca aagacggcaa
     1381 accatctgtg gagatcacac ctgcgtatcg cggttggacg tactgagttg acgcgccacg
     1441 caaccaatgg aagatgaacc atgcctgagg taatccagga tgaattgttc gaaggcgcgc
     1501 ttaaaaccgg tcgccccatc tctctttatc tgaaaaccgg agtgcccgtg cgcggaaaag
     1561 tgcgcgctca tgatggattc acggttctcc tggaacagga aggtcgcagc attctcgtct
     1621 acaagcactc gatcacttcc gtactcgggg cacgccccgg acaacgcaag aaatgaagca
     1681 tcttcgcatt ggagtggacg cgcggccttt gagttcgcgc gtttccggtg ttgctcgtgt
     1741 gatcgcaagc gtattgcgtg aactatcaga cgatcacgag ttcttcctct attcagcgca
     1801 gaatccacat gcagactatg ctgaaattct agctcacaag aagattcact ggatgcaggg
     1861 acagggcctt cttgcctgga agcaaggttt gtggtttaac tctgctctgc ctgctactct
     1921 cgggaaagag aatctggatt tgttctgggg ttcacagcaa gttcttcctc catttctgcc
     1981 gcggattcca gcggtgctta ctttttatga tctggttctg ttgtattttc ccggagctat
     2041 gcgacgcatt gcgcgtatcc agcagcgcgc atttctgaac tacagcgtaa aaagagccag
     2101 tcgcattctt tgtatttcag atcaatctcg tctggacatg attcgagaac tcggtgtgcc
     2161 agaagaaaag acagcagtgt ctctgctggg tgtggatcct cccgcgaaaa gaaagaaatc
     2221 ggaacagaga aaacctcctg tgaatggacc attcattctg agtgtttcca ctcttgagcc
     2281 cagaaagaat tacggaatgc ttctggatgc gtatggccag tacgcatcgc atgcgatggc
     2341 acgtgcgaaa ccttatcccc tggttctggt tggaagacgc ggatgggagt ccgctgattt
     2401 ttaccagaag ctaaagggca tggaggacac cggccttgtg cacgtgcttg attccgaaag
     2461 cgatgagtct atttcggatc tgtatgagca atgtgcattc tttgttcttc cctctctgta
     2521 tgaagggttt ggcctttctt tattggaggc gatggccagg ggcaagcatt gcattgcatc
     2581 tgaccttggt tgctttcatg agatcggtgg aagccaggtt acctatcttg ggtcaaaaga
     2641 tgtgcatgcc tggagagacg ctcttgcgga cgcggtctcg aagcatcgtt ccggaaaact
     2701 gcgtcctaca aaaataaaac taagcgattg gtcctggaga aatacggcgc tggttcacgc
     2761 agatgctttt gcagctgtag tctgaccgtc tgtgatcatt tcgcaattgc catcaaagac
     2821 gactccgtct gcgatctgca attttgcagt cttcacgttt ccgtagacct ttcctgatgt
     2881 gagcatttcc agcttttgcc tggcttcaat attcccggtg acttccccgg ccacaatgac
     2941 gatgcccgcg cgaatgttcg cttttactgt cgcgccttct ccgaccacga gcacgccgtc
     3001 tgtgatgagt tcgccctcaa agtggccgtt gatctgcagt ggtttcttga attccatcac
     3061 gccggagaag gaagtctcct ttcccaggat ggtggaaatc ggccctcctt cagtgatgga
     3121 gggggattct tttttcttga gcatgttatt ttgtcttcat tacatagact ccgtcccagt
     3181 cttcacctgg cgggtctttg atgtatgcgt cacatcgttc cacgtagagt ttggatggac
     3241 cgtcttctgg caggatctgc agacctttga ggaacgtttc gcgtgcctct ttgaatttgc
     3301 gcgctttgta gagcccgagg ccctgattgt aaagcgctac gacctcttcc tgctgttttg
     3361 tgagcatatt tgttcttctc agtcgggggg cgcgcttgca atcaaaattt ctcgattaga
     3421 tcgagtgccc ggcccaccag ggatagcatt tccctttccg gcggagatgg ttccgcgaat
     3481 gcctggacga gctggcgaac atatggaatt tttcgcatgc ccacgagtgc cgtggcgggc
     3541 acatctccgg acaacagaat caggagcact tgaagggcca ggggtttgcc ctgcagagcg
     3601 ggatgttctc ccaggagatt ttcgagaccc tccattctct ggtgaaccag aatctccaca
     3661 tagcgctccc agtagtggat cacagcgttg actactttta gatactgatt gaatccaatt
     3721 tcctgatttt ttgtttgaac tgctgtctgg attcttcggg cggtcgggct gaacaaatgc
     3781 gtaatctgct ccacagacgc acgcagttga tctgtggtcg tgaatctatc gcggtatctc
     3841 tctaaaatat cagaaaacgc cggcgcggtt ttgtcgaact ggaaggcaga accaaacaga
     3901 tcatgaagct gagcttcgag ttccatcaaa cgaccctgca ttttttgaaa ctcagcaatt
     3961 cctgaatctc ctccgtccgg tggagcatcc acgaggcgtg ccaggcgaat cagtccgcgc
     4021 ggaccgtttg cattgaatgg ccgattgccc agagtccaga gtccgtgggc aagagcgact
     4081 gacgttacag ttcctcctcc tgcagagcga ttgtatctaa agtcgttctc aatgagattg
     4141 gctggaaact gaactgccgc aaagcgaggg ccagctaccg agaggacttc gtcgagacga
     4201 gtggccgtgt aactggaaga tgaatgggcc agagtgttgg aggatattcc gtagtactga
     4261 atgattccac gattggccat ttcctccatc atctcaaagg cgcggcgcaa tcgatccacg
     4321 tactgagcac gggcctcatc tgccaggacg cctcgtgttt gtgcgtctat gagaaagtat
     4381 tctggattgt gaagtagcaa gaagtcgatg gtgcgaagcc ccagtctgta gcgcgcgagt
     4441 tgcagttgcg atttgatgta gtccggatgg atgcagtgcc agatgtcatc gcgcactttc
     4501 acaacttctg gcggaggagc ggtccgcagc aggccgaggt ttcttccctg gatgtatccg
     4561 atctttgtaa ccacagcaac ttgattgcgc tcgattcttt ttgcttccac gagctgtctg
     4621 attacatctc ccaccatttg ctcggaagat ccgtccgagt aattggcggc ggtatcgatc
     4681 acattgactc ctgtaaggat agcttcccgg agagcgtcta catttgcgcg cgcgccagca
     4741 ctcactctgt agcagccaaa cccgatgcgc gaaagtgcca gatgcgatgg cgtctggaat
     4801 ctaaaccagg aatggtctct gtattctgcg aattgtgttt tcagatccag gaagtagcga
     4861 gcggtccctt cttttgtggc cgctccttcc atcaggccga catgaagatc ctcggctctt
     4921 tttcctgagc tcatccagct ggattgaagt ttcgaatttg gacctgaatg cgagcggcat
     4981 cctcgtactt ctcctcgaga attgccttga acttgtccag gtacagagca ctgagtttca
     5041 ggtcttcctg gttgaccgcg ctttcgaggt tgatcatatc gagttgcggg tgctggtgct
     5101 ttagaaattc aaccgcggcc tggatgcttt ccttgctttg aagcccgtcc gtgattgcct
     5161 gctcaatggt tgcgatggca tgcgcatcgt ccatgtccgg tgcttgaatc aagagaggct
     5221 gttcgggaaa aagcggcagt ctttgctgcc cgtgtttttt gcgctttcta tccagctgct
     5281 tgcgatagat ttccacatat tcataaaccc ggtcattgaa acgctccagg cgttcactca
     5341 tgtagtccgc ttctggcttt ccttccggaa tttcgaaatc ttcgaggcga actacatcgc
     5401 tgtcttcgtc cagatctaac agttcaaaga attcctcttc tgaggtaaga gctgacgggg
     5461 gaaatactac gaccgggaaa ttgttgtttc gcacacccag gtagctgacc accacatggc
     5521 agaccaggag cttcgcgtcg ggcgaaaaag gattgggccc gcgcacccgg cagtaaaaca
     5581 gaaggcttcc ggatggtgtt tccgaattgc cgcgataaaa ctcaagcatt ccgctcacta
     5641 gaggctttca ttataaggca aaacggtgcc ggcgtcaagt tccattcttt tccgcctcga
     5701 gctcttccac gcgatactgg ctgatcggaa gggactccgg tcttttcagt tttttgttgc
     5761 cctgtttgac gatttttccc ggagttccga ccacagtaca gttggcaggc acgtcccggt
     5821 tgatgattac agtttccgct ccaattcgcg catgatcgcc tacgtgaatg ggcccgagaa
     5881 gagtggcatg agtgccaatg agcacatcat ttccaatgat gggatgtctt ctccctttgt
     5941 gtttgccggt tccacccagg gtgactccgt gaaacatcat acagtccctg ccaatgatcg
     6001 ccgtttctcc gatcactaca cccattccat gatcgatgaa gaatccgccg tcaattttcg
     6061 cacccggatg gatttcgatt ccggtgaaga acctggacat ctgagagaag aagcgcgcga
     6121 agaatcgcag acctacgttg tagagtggat ggcaaactag cctgtgccag aaaatcgcat
     6181 gcagacatgg atagagcaaa aactcaagtc ctctggctgc cgggtcattt cgaaagatgg
     6241 agcgtacgtc ttcgacaatt ctaagtcgca ttgttctttt ctagttcctt gaacagaagg
     6301 ctgggctgga tgccagtgtt gttctggtat tttcctgact tgtactcgag cacttcgcct
     6361 tctatagtat gataaaaaat ctgacagatg ggaacaaaag gataaatgcg aatgggctgg
     6421 atggcagaaa tttccagagt ccagaatccg cgaaacccaa catcaccaaa cccggcagtt
     6481 acgtgcacga atagaccgag tcttccaatg gaagatctgc cttcgagcat gggcaccaga
     6541 ttgaatgttt ccgtatactc aactgttcta ccaagataga gagttccagg ctcaatgacg
     6601 atcccttccg gtgggatctt gatcatttct gtttgcgccg gcttcttcat gtcgagcgct
     6661 cgctctttgt agatcatcag ctcgtcattg agtctgaggt tgtatgaatt cggattgatc
     6721 agagacgcat cgaatggttc gatcttgatt tctgttccca tcctgcgaac aatttccggc
     6781 cccgttaaga tcatctcttg cctctgtatg cttcagttgt tgtttctcta ctattcgccg
     6841 gtgaacctgg ctgctctctt ttcgcggaac gcatgaagtg cttcgattct gtccttcgtt
     6901 ggaattgtga tttcgtatgc atttctctcg attgccaggc cagtggacag gtctacattc
     6961 gcgcctgcgt ttatggcaaa ctttgcagat cgaaccgcaa tgggagcatt ttttgaaatt
     7021 gagctggcca gttcgacgca agcttcatcg agtttttccg cgtttacgac tgaatcaacc
     7081 agacctatgc tcaatgcttc ggatgcgccg actttgcgtg cggtgaatat gagggctttc
     7141 gcacgggaca ctccaatgag tctgggcagc ctttgagtgc caccggcacc cggtatgatt
     7201 cccagagaag tttctgtaag cccgaggagc gcatccgtct gggcgattcg aaagtcgcat
     7261 gccagagcca gctcgaggcc gcctccgagt gcaaagccgt tgatgccgca gatgacagga
     7321 cagggaaagg attcaatggc ctggaagata tgaccgactc ggatgagaaa atcgcgcact
     7381 tctggcgggc tcatggatgc tctttctttg agatcggcac ctgtgcagaa agaccgtccg
     7441 gaccccgtta gaaccagagc tcgtatggcc gggtccacag agagcgcctg gattctggtc
     7501 tcgagcaatg ccagaagctc gcgactcatt gcattgagcg cgtcaggtct attcaagcgc
     7561 agtcttatgg tatgctcgat tctttcttcg atcagtaggc tcatgctgca tcgtttttgt
     7621 gctcaggggc ccggacaagg actttcgcct tttgaccgat gggtgcctgg ccggctcttt
     7681 gatctgtaaa aattggccga tattagagcc agggccaaaa aagccttgta cattagaacg
     7741 aag
//
LOCUS       DAORFF010000118         7737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4683_length_7737_cov_3.372559, whole genome shotgun sequence.
ACCESSION   DAORFF010000118 DAORFF010000000
VERSION     DAORFF010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4683_length_7737_cov_3.372559"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /gene="ftsZ"
                     /locus_tag="PKK76_06465"
     CDS             complement(<1..1061)
                     /gene="ftsZ"
                     /locus_tag="PKK76_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNL68148.1"
                     /translation="MMQLVEDGTSPALIKVVGVGGGGMNAVNRMIDAHLRGVEFLVIN
                     TDEQVLRKSRVETRIAIGQKTTRGMGAGGDPEIGLRAAQEDRDRLLQSLKGADMVFIT
                     AGMGGGTGTGAAPIIAEVARELKALTVGVVTVPFEVEGKRRRELALKGLAALRDKVDT
                     LIVIKNDSIFKVIDQNTPVDVAFRIVDEVLLGAVRGISDLVNEAGLINVDFADVRSVM
                     AEKGEAVMGAGEGMGENRVVEAVSQAINNALLEEHSVEGATGVLINVCGGEDMTMKEW
                     RDVSELITQRVDPGANTIIGMTIDPALRDRIRVTVIATGFKRNAAQGAPRKNSIFPES
                     KKPEEPPVRRPYFPAATAAP"
     gene            complement(1133..2350)
                     /gene="ftsA"
                     /locus_tag="PKK76_06470"
     CDS             complement(1133..2350)
                     /gene="ftsA"
                     /locus_tag="PKK76_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020765074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNL68149.1"
                     /translation="MKEFASIDIGTSYTRVLMGRMGDDGVIQINGAGVSPSQGVRNGG
                     VVNIEQTVQSIQTAAREAELMSGLVVEEAVVNITGKHLKGENSRGVVAVTNRDRVVTE
                     SDVLRVIEGAQNIRIPADQEILHVLSREFVVDDQSGIRDPIGMTGVRLESEVHIVTAG
                     KTALTNLRKAVNAAGIRVTNLVMSSLASSEAVLTSGEKDLGVAVVDIGSGVTDIIVYV
                     DGGVFSSATLPWGGSHVTQDLSFGLKIPMEAAELVKRNSGCVQIAAVDPIEKIELPAM
                     SGRPPRWVLRQEIAAIMEPRMREIFELVDQELMRSGKKNALSGGVVITGGGSRVEGAA
                     SLAEEVFSMAVRKGNPLPLGGFFDRVDGPEFATSVGLLIGAAQSTDTARKAPRESGSG
                     WMQKLRSWVSENL"
     gene            complement(2347..3069)
                     /locus_tag="PKK76_06475"
     CDS             complement(2347..3069)
                     /locus_tag="PKK76_06475"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNL68150.1"
                     /translation="MLEQNSSRRQVYVVAGLVSVLLIGAGTGLVFYYGKTYTNVKHIR
                     VAGNVMLSASEVLAAAGIDLARPFTRGDMYSAQNRLLSHPAVASATLQEEGEEVIIRI
                     RERACLAVLRVDGSMIDVDETGRILFYDGRCSASPLVSGAFRIQDNRVHGEGMADVLS
                     ALRALRDGRPDLENRVSEIHLRKGYYTIYVVRPAIQIDVPDLSQENIERITASLAFFE
                     KEGQHRGRIDLRGKNALYLPKT"
     gene            complement(3145..4464)
                     /locus_tag="PKK76_06480"
     CDS             complement(3145..4464)
                     /locus_tag="PKK76_06480"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNL68151.1"
                     /translation="MKVYFIGMGGSGMLPLARLAREFGHTVAGSDPRGAADLDVFSNV
                     DPSRIDAFDSVVYSSAIPPEHPELQYARSRGEFVKVYHRMDFLTELMRYSEVRIGIAG
                     THGKTSTTSLLGWVLLDQGLDPTIFAGGKPLYLKEGMRKGNGPAVFETDESDGSFLRS
                     NANFKICLNVDEDHLEHYGTYRHLIDAFRSFASSGTVVLNGLDSSLPNIPGAIYFAAL
                     EAGQESRADYTGRFLDETDALVVSARGTEVGQLSIPLPGRHFASNALGVFALLDTMIQ
                     QGLLSGLNRKGILESMSRFPGVERRMERIGTAQGSPVFDDYGHHPSEIAAVIRALRVR
                     YPDKRVKIVFQPHRYTRTRDHASAFAEVLKLADEASILPLYSAGEKPIDGIDSEMIAR
                     QTQARLIQEADIPELLDRAGPGDVLLFQGAGDISAMVRRAIASRKST"
     gene            complement(4461..5495)
                     /locus_tag="PKK76_06485"
     CDS             complement(4461..5495)
                     /locus_tag="PKK76_06485"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNL68152.1"
                     /translation="MRVLIVAGGTGGHISPGVALVQEFVRRGVETAILTLRKNEKYPD
                     IGSAGVPVLLYNAPPFPSSILSLIRYPFKMLGAFLYAFRALRTFDCVIGLGGYPTIPG
                     LLAALTRRKQIYLCEQNAVPGQVTRLFSRFASAVFLSFPIERRIPRSELVGNPVRSQL
                     IANRKRNAQNGLILVLGGSQGARQINQMLTALLLDESRPWTKEATWLIQCGESHLESM
                     REMLGARDNVTLFGFHSSIQELYAKAAVLISRSGAGILTEGTLFGLPLVLIPLPTSKD
                     DHQRANARVAEAAGAAITIDQNGADHQRLADVLGKLLTSPEILEDMSKKSAALFAADA
                     ASRIAERVLA"
     gene            complement(5492..6643)
                     /locus_tag="PKK76_06490"
     CDS             complement(5492..6643)
                     /locus_tag="PKK76_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNL68153.1"
                     /translation="MERLKWGPSFSEIEAAVTAKRDVVLFALILLLAGMGLFALHPAS
                     SLQARIDQGDGDYYFTRQMVFALPGLVGLFFFAYVPLDFLRRYSLLFLAGCAFLLLLV
                     FVPGIGQKVSSRSESFHRWIAFGPVRFQPSEFAKVSLIIYAAHILGKFNLASPDADLR
                     KLIWPFALMGILLFLILIGPQYGTTICTLSVLAVMIYLSGFPMLRLMLVGLASLPLIG
                     LGIFLWSYRLERFRVWLDPYSFRHEGGYQLVTAFRAFHDGGLTGVEIGTGFAHRYLTF
                     GHTDFILALFAEDFGILGLSLLFLLYVAFVWRSVSLLRRVNHPFYFMLGAGCLVMIIV
                     QSLLNIAVVTGVVPTTGVSLPFFSYGGSSLIATFCMTGLLLNACGQVDS"
     gene            complement(6631..>7737)
                     /gene="murD"
                     /locus_tag="PKK76_06495"
     CDS             complement(6631..>7737)
                     /gene="murD"
                     /locus_tag="PKK76_06495"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNL68154.1"
                     /translation="LSLDIFSSARERGIPVFGENDLGAHLISTSWNPPPFVCAVTGTD
                     GKSTTTALIDHMARSVGISSVACGNFGTPLSSLVFDPPVRMLSLECSSFQLEPARFLR
                     AHVAMILNLSQDHMDRYATLEDYLHAKLNVTMNQTAEDLFIAPKWILDRSTTPARKIA
                     LESLQYVRDEIWFSGKAVARLADYSLPGAHNRINLNFALAALENASGRLGISVSAESI
                     RSAIASFRGLPHRMESVVTVRGIEFVNDSKATTVQAVCSGLTAFAGRSVALLCGGYDK
                     GLDYSELAKFEGVVLLPFGEAGPKIARDTGAARVYATLEEALRAGADLCKSQGQGVVY
                     LGPACASYDAYNSYEERGEHFRSLAMGLAKEWNA"
BASE COUNT         2003 a         2177 c         1801 g         1756 t
ORIGIN      
        1 ggagcggccg tagctgcagg aaagtatggc ctgcgaacgg gtggctcttc tggtttcttt
       61 gactcgggaa aaatggaatt ctttctgggc gcaccctgag ctgcgtttcg cttaaaaccg
      121 gtggcgatga ctgtaacgcg aatccggtcg cgcaatgctg gatcaatggt cattccaatg
      181 attgtattgg ctcccggatc cactctttga gtgatgagtt cgcttacgtc gcgccactct
      241 ttcatggtca tatcttcgcc gccgcaaacg ttgatcaaaa cacctgtcgc gccttccaca
      301 ctgtgttctt cgagaagagc attgttgatt gcctggctca ctgcttctac tacacggttt
      361 tcacccatgc cttcgccggc acccatcact gcttctccct tctccgccat cacactgcga
      421 acatccgcga aatctacatt gatcaggccc gcttcattga ccagatcaga aatgccgcga
      481 acagcaccca ggagcacttc atctacaatg cgaaacgcaa catccactgg agtgttttga
      541 tcaatcactt tgaagataga atcgtttttg attacaatca gtgtgtctac tttgtctcga
      601 agtgcagcca ggcccttaag agccagctct cgtcttcgct ttccttctac ttcaaaggga
      661 actgtgacca caccgacagt cagcgccttt aactcgcgag ctacttccgc gatgatcgga
      721 gctgcacccg ttccagtgcc acctcccatt ccggcggtga tgaataccat gtctgctccc
      781 ttcagagatt gcagcaacct gtctctatct tcctgggccg cgcgaagacc gatctcagga
      841 tctccacctg cacccatgcc gcgggttgtc ttttgtccaa tggcgattct ggtttccacc
      901 ctggattttc tgagaacctg ctcatccgtg ttgatgacca ggaattccac gccgcgcaga
      961 tgagcatcaa tcatgcgatt gactgcattc atgccgccac cgccgactcc aaccactttg
     1021 atcaatgccg gtgatgttcc atcttccaca agctgcatca tatctcattc ctctatatat
     1081 attgtatcta tttctatact ttcaaatttc tttatgattg cgtttctctg tttcacaaat
     1141 tttcggagac ccaggagcga agtttctgca tccatccaga gccagattct cgcggtgctt
     1201 tacgagctgt gtccgtagac tgggcggctc cgatcaaaag tcccacagac gttgcaaatt
     1261 ctggcccgtc cactctgtca aaaaatccac ccagaggcaa aggattgcct tttcgcacgg
     1321 ccatagagaa aacttcttca gcgaggctgg ccgccccttc tactctggag ccgccacccg
     1381 tgatcacaac accgccagaa agagcgttct tcttgccaga tcgcatcaac tcctggtcca
     1441 ccagctcaaa gatctctcgc atgcgcggct ccatgatggc cgcaatttcc tgacgcagta
     1501 cccagcgtgg tggcctgccg gacatcgcag gcagctcaat tttttcaatt ggatctaccg
     1561 ctgcaatctg cacgcatccc gagttgcgct tcactagctc cgcggcttcc attggaatct
     1621 tcagaccaaa agaaagatcc tgcgttacat ggcttccgcc ccagggcaaa gttgcagaag
     1681 aaaagactcc accgtccaca tacacaatga tgtccgtgac gcccgaaccg atgtccacca
     1741 cagcgacacc caggtctttt tcccccgacg taagaactgc ttctgaactg gcaagagaac
     1801 tcattaccag atttgtaaca cgaatgccag ctgcgttgac tgccttgcga agattggtga
     1861 gcgcggtctt tccggcagtg actatgtgca cttcgctttc gaggcgaacg cctgtcattc
     1921 caattggatc tcgaattccg gattgatcat ccactacaaa ttcgcgcgaa agaacatgca
     1981 atatttcctg gtctgctgga atgcgaatgt tctgcgcgcc ctctatgact cttagcacgt
     2041 ccgactctgt aacaactcga tcgcgattcg tgacagcgac cactccccgc gagttttcgc
     2101 ctttgagatg tttgcctgtg atgtttacaa ctgcttcttc tactacaagc ccgctcatga
     2161 gctcggcttc gcgcgccgcg gtttgtatgg actgaacggt ttgctctata ttcacaacgc
     2221 caccgtttcg cactccctgc gaaggagaca cgcccgcgcc gttgatctgg atgacgccat
     2281 catcgcccat tcgccccatc aaaacgcgag tgtatgaagt gcctatgtca atggatgcaa
     2341 attctttcat gtttttggaa gatacagtgc atttttgccg cgaagatcga ttctgccgcg
     2401 gtgctgccct tctttttcga agaatgcaag ggatgccgtt atgcgctcaa tgttctcctg
     2461 tgacaaatcc ggcacgtcaa tttgaatggc cggccttact acgtaaattg tatagtaacc
     2521 tttgcgaaga tgaatctcag aaactcgatt ttcgagatca ggccttccat ctcgcagtgc
     2581 gcgaagagcg gacaaaacat ccgccatacc ttcgccgtga actcgattgt cctgaatgcg
     2641 aaacgctcca ctgaccaggg gcgaagcact gcaacgacca tcatagaaca gaatccgtcc
     2701 tgtctcgtcc acatcgatca tagatccgtc cactctgagg acagcaagac acgcccgttc
     2761 acgaattcgt atgattactt cttcgccttc ttcctgtaag gtggcgctgg caactgctgg
     2821 atgagagagc aaacgattct gcgcgctgta catatcgccg cgcgtgaatg gtctggccag
     2881 atcaatgccc gccgcagcga gaacttcgga tgcagagagc atgacattcc cggccacgcg
     2941 aatgtgctta acattcgtat aggtcttgcc gtagtaaaaa acaagcccgg tgccggcacc
     3001 gatcaacaga acggatacaa gaccagcaac cacgtagacc tgtcttcttg agctgttttg
     3061 ctctagcatg cgttcggaaa atattatgtc gcattgcatg cgcaaccaaa aaaaccagtt
     3121 tgccctgaaa gggcctggaa aaactcaagt gctcttgcga cttgcgattg ctcttcgcac
     3181 catagcggag atatctcctg ctccctggaa caaaagaacg tcgcctggcc ccgcacgatc
     3241 gagcaattct ggtatatctg cctcctggat caaacgagcc tgtgtttgtc tggcaatcat
     3301 ttctgaatca atcccatcga ttggcttttc tcccgcagaa tagagaggca gaatagaagc
     3361 ctcatctgcc agtttcagca cctctgcgaa ggcagaagca tggtctctcg tgcgcgtata
     3421 acggtgaggt tgaaatacga tcttcactcg cttgtctgga tatcgaaccc tgagtgcgcg
     3481 aataacggct gcaatttcgg aaggatgatg cccatagtcg tcaaaaacag gagaaccctg
     3541 agctgtgccg atccgctcca ttcttctttc tactccgggg aaacgagaca tggattccag
     3601 gatgcctttt ctattgaggc ctgagagtag cccctgctgg atcattgtgt cgagcaaagc
     3661 gaacactccg agagcattcg atgcaaagtg ccgaccggga agcggaatag aaagctggcc
     3721 cacttccgtc cctcgcgcag acactacgag tgcatctgtc tcatctaaaa agcgcccggt
     3781 gtagtcggcc ctcgactcct gcccagcctc gagcgctgcg aaataaatcg cccctggaat
     3841 gtttgggaga gaagaatcca gcccattcaa aactacggtt ccggaagaag caaaggatct
     3901 aaacgcatcg atcaaatgac gataggtgcc gtagtgttcc aggtggtctt cgtccacatt
     3961 caaacatatc ttgaaatttg cattggatct gagaaaggaa ccatccgact catccgtctc
     4021 aaacacagcg gggccattcc ctttcctcat tccttctttc aagtagagag gttttccgcc
     4081 cgcgaaaatg gtaggatcaa gcccttggtc cagtagaacc cagcctagta aggaagtggt
     4141 agaggtcttt ccatgcgttc ccgcaattcc aatgcgaact tccgaatatc gcatgagttc
     4201 cgttaaaaaa tccattcgat ggtaaacttt tacaaattct ccgcgagacc gtgcgtattg
     4261 caattccgga tgctctggcg gaatcgcaga actgtacacc acagagtcaa atgcgtctat
     4321 gcgcgaggga tcaacattcg aaaacacatc cagatctgcg gctcctctgg gatccgaccc
     4381 tgccacagta tgaccgaatt cgcgcgcgag ccttgcgaga ggaagcatgc cggatccgcc
     4441 cattccaatg aagtacactt tcacgctaag actctctccg cgatgcgcga agctgcatcc
     4501 gcagcaaaca gtgccgcact ctttttggac atatcttcca ggatttctgg cgacgtgagc
     4561 aatttcccga gcacatctgc cagcctctga tggtccgctc cattctggtc gatcgtaatg
     4621 gcagctccgg cagcctcagc cacgcgcgca ttcgcacgct ggtgatcgtc tttggacgtt
     4681 ggaagaggaa tcagaaccag aggaagtcca aacaacgttc cttctgtcag aatgcctgca
     4741 cctgatctgg atatcaaaac cgccgccttc gcatacagct cctgaatcga agaatgaaag
     4801 ccgaataacg tcacattgtc cctggctcct agcatttcgc gcatggattc caggtgagac
     4861 tccccacatt gaatgagcca ggttgcctct tttgtccatg gcctgctctc atccagtaga
     4921 agagccgtga gcatctggtt gatctgacgc gcaccctgac ttccaccgag tactagaatc
     4981 agaccgttct gtgcgtttct ttttcgattc gcaatcagct gagatctaac tggatttcca
     5041 accagttcag aacggggaat gcggcgctcg atcggaaaag atagaaacac cgcactcgca
     5101 aatctagaaa acagtcttgt gacttgacca ggaactgcgt tctgttcaca caaatagatc
     5161 tgctttcttc gcgtgagcgc agcgagcaag ccagggatcg ttggatatcc gcccagacca
     5221 atcacacaat cgaatgtccg aagcgctcta aatgcgtaca ggaatgcgcc aagcattttg
     5281 aaagggtaac gaatcagaga gagtatggaa gacggaaagg gaggagcatt gtatagaagc
     5341 acaggaacgc cagcggaccc aatgtcagga tatttctcat ttttgcgaag ggtgaggatg
     5401 gctgtttcca ctccgcgtcg aacgaattcc tgaaccagag caacgccagg tgaaatatgc
     5461 ccgccggttc ctccagccac aatcaatact ctcatgaatc cacctgcccg catgcgttga
     5521 gtaaaagtcc agtcatacag aaagttgcaa tcagcgaact tccaccataa ctgaaaaatg
     5581 gaagactcac gcctgtggtt ggaaccacgc ccgtcacaac tgctatattc aaaagactct
     5641 gcacaatgat catgaccaga caacccgcac ccagcatgaa atagaaagga tgattcacac
     5701 ggcgaagaag cgaaacagat ctccagacaa atgcaacata cagtagaaaa agcaaagaaa
     5761 gcccaaggat tccgaaatcc tctgcaaaca aagccagaat aaaatccgta tggccgaatg
     5821 tcagataacg gtgtgcaaat ccagttccta tctccactcc agtgagccct ccatcgtgaa
     5881 aggcgcgaaa ggcggtcacc agctgatagc ctccttcgtg gcgaaaggaa taaggatcca
     5941 accacactct aaatctttcc agtctatagc tccacagaaa gattccaagg ccaatgagcg
     6001 gcaaggacgc aagaccaacg agcatgaggc gaagcatggg aaagccggag agataaatca
     6061 tcacagcgag aaccgaaagc gtacaaatgg tggtcccata ctgtggcccg atcaaaatca
     6121 aaaagagtaa aatccccatc aacgcaaaag gccagatcaa tttacgcaaa tccgcatccg
     6181 gactcgccag gttgaatttt cccaggatat gcgccgcata gatgatcaga cttaccttcg
     6241 caaactcgga tggctgaaat cgtacagggc caaacgcaat ccatctatga aaactctccg
     6301 atctggagga taccttctgc ccgattccag gaacaaagac aagcaggagc aaaaacgcgc
     6361 atcccgccag gaacaagagc gaataacgtc tcaggaaatc gagcggcacg taggcaaaaa
     6421 agaagagccc tacgagcccg ggcaaagcga acaccatctg gcgcgtgaag tagtagtctc
     6481 catctccctg atcaatgcga gcctgcagcg aagaagcagg atgaagagca aacaatccca
     6541 ttcccgccag aagcaaaatc agagcgaata atacgacgtc gcgctttgca gtcacggccg
     6601 cttcaatttc ggaaaatgag ggcccccact tcaagcgttc cactcctttg caagacccat
     6661 tgccagagaa cgaaagtgct cccctctttc ttcataagaa ttgtaggcat catacgaagc
     6721 acaggctggc ccgagataaa ccactccctg cccttgactt ttgcacaaat ctgcacccgc
     6781 gcgaagtgct tcttcaagag tcgcataaac gcgagcggct ccagtatcgc gcgcaatttt
     6841 agggcccgct tctccaaatg gcaaaagcac cactccctca aatttcgcaa gttccgaata
     6901 gtcgagccct ttgtcgtaac cgccgcacaa aagagccaca gatcgccccg caaaagcagt
     6961 gagaccggag cacacagcct gcactgtcgt tgctttggaa tcgttgacaa attctattcc
     7021 ccgcactgtg accactgact ccattctatg tggcaatccg cgaaaagacg caatggcaga
     7081 tcgaatgctt tccgccgaaa cgctgatacc cagacgccct gatgcattct ccagtgcggc
     7141 gagagcaaaa ttcaagttga tccgattgtg cgcgcccggc aaagaatagt ccgcgagtct
     7201 tgccaccgct tttccagaaa accagatctc atcgcgcacg tactgaagag actcaagggc
     7261 tatcttgcgc gctggcgtcg tggatctgtc caggatccat ttcggagcaa tgaatagatc
     7321 ttccgcggtc tgattcatag tcacgttcaa cttcgcatgc agatagtcct ccagtgtcgc
     7381 ataacggtcc atgtgatcct gactcagatt caaaatcatg gcaacatgag cgcgaagaaa
     7441 acgagccggt tccagttgaa acgaagaaca ttccagagaa agcatccgca ctggcggatc
     7501 aaacacaaga gaagaaaggg gggtgccgaa gttcccgcag gcgacgctgg aaattccaac
     7561 agaccgggcc atgtgatcga tcaatgcagt ggtagtggat ttgccatccg tacctgtcac
     7621 agcacataca aacggaggcg gattccagga agtggagatc aggtgcgctc ccagatcatt
     7681 ctctccgaat acggggattc ctctttcgcg tgcgctagaa aagatatcaa ggctgag
//
LOCUS       DAORFF010000119         7663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4744_length_7663_cov_3.388538, whole genome shotgun sequence.
ACCESSION   DAORFF010000119 DAORFF010000000
VERSION     DAORFF010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4744_length_7663_cov_3.388538"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="PKK76_06500"
     CDS             complement(<1..1215)
                     /locus_tag="PKK76_06500"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNL68155.1"
                     /translation="MQPAESALDILSRRAGISLEYHDIDGVAHTTSEEAKRTILEILG
                     YSGDPSAILSDLIQADATTLIAPAVIASVYAPRFNMRIPVDIVRQIELRIELEGGITV
                     GLRPQFELKEEHLVAGRTIGLYEVDLKDSIPPGQHIISVMRWKEEIGRSTLFLCPESC
                     YLNAEIENGSRWNGLWLQLYALRSSANWGIGDLGDLLELARLVGPRGIRILGLSPLHA
                     PFLMRPDFKSPYSPSSRLYKNAMFVSIPLLPEFAASAKARSLFESSEFQLYLARTRPE
                     ERIDYAGVYQWKMRFFEILFDEFISDPPEDFAEYVEEGGIELSRQAIFDCLEERFDYN
                     LDRPAGFHGWPEAFQDASSDAVRAFADASHRRVQFYIFLQYLFDRQFSDVSGACLDLG
                     VCLYADLAVGSHP"
     gene            complement(1217..3310)
                     /gene="glgX"
                     /locus_tag="PKK76_06505"
     CDS             complement(1217..3310)
                     /gene="glgX"
                     /locus_tag="PKK76_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNL68156.1"
                     /translation="MAGEPYPLGATWDGSGVNFSLYSEHGTGVELCLFDSADAKKESR
                     RISMTHRTDMIFHCYLPDVRPGQIYAYRVYGPWEPAKGHRFNPNKLLADPYAFAMARS
                     VLWDDSLYAFDPNNPSRMDERDSAPFAALCAVVEKAFTWGDDRPPRIPWRDTIIYEAH
                     VKSLTMRHPEVPQRMRGTYSGLASEPMIEYFKSLGITAIELMPVHHHVDEHVLAMRGL
                     KNLWGYSTLSYFAPDLAYSAGRRVGDAIQEFKVMVKALHRAGLEVILDVVYNHTCEGD
                     HRGANLCFRGIDNASYYRLDPNNPGMYTDFTGCGNTLNMQNPRVLQLIMDSLRYWVTE
                     MHVDGFRFDLASALGRELHAVDRLSAFFDIIHQDPVLSRVKLIAEPWDLGEGGYQVGN
                     FPVQWTEWNGHYRDCVRSFWIGGNVAREFATRLTGSSDLYEAAGRRPYASINFVTCHD
                     GFTMLDLVSYDRKHNEANGWNNTDGNDHNISFNHGTEGETQDPTIRATRFRQMRNYFA
                     TLLLSQGVPMINMGDELARTQKGNNNAYCQDNDISWIDWDLTEEQKHHLEFVKKLIHL
                     RRQSRVLRRNHFIRGGEPGEERDMIWLHPDGHEMKSSEWYDSMPIFGAIIDGLHPQEN
                     KKFYEIGGKDEDVEPLLIVIARSAGEFRIPAEPNLSWYRILDSELGPSISVKAGTSLD
                     LMALTVSVFRPDRRG"
     gene            3440..4735
                     /locus_tag="PKK76_06510"
     CDS             3440..4735
                     /locus_tag="PKK76_06510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNL68157.1"
                     /translation="MSNYKIFMIGWEYPPRITGGLAVACEGIASSLGRRGNSVRFLVP
                     RMHGDEPVHENVELVDAGTRTELYTTDELEVLRSMALHDFTTRQVFSPYASSEYSGQS
                     LVDITEKLAVMQKQSREIPLLRGGYGDWMYQEIERYARAAYFIAQKTEMDVIHAHDWM
                     TFPAGLACMNATGAPLVVHVHATEFDRSGESVNQYVYDLERYTFHHATAIMTVSNYTK
                     NVLVQRYGVSPEKIFPIHNAVEFELPGAVLERKKALKDHIVLFLGRITFQKGPDYFVR
                     AAQLVLQQIQNVRFVMAGTGDMYSRMIEYAADLGIGKFFHYTGFMNRAQVKRLYSMSD
                     LYVMPSVSEPFGISPLEAMMHGVPVIVSRQSGVSEVIQNCIKVDFWNVEELAEQILRV
                     LHDTELKQNMQQEGHREVQAISWDAPAEKMEQVYGRLVK"
     gene            4732..5913
                     /locus_tag="PKK76_06515"
     CDS             4732..5913
                     /locus_tag="PKK76_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNL68158.1"
                     /translation="MIAVCFYFEVHQPFRLRPYHFFDVGVDPHYLDEQKNRDICRKVA
                     NKCYLPANKVLLDLIKLYGSDFRVSFSITGTAIEQFRMYSPETLDSFRKLADTGQVEF
                     LNETYYHTLASLYSENEFRAQVAKHGQLMREEFGYNPVAFRNTELIYSNHIAWLAREM
                     GFQTILLEGIERVLDWRSPNHVYAPEHSPGIKCLLKNYRLSDDVAFRFSDRNWKDWPL
                     TAEKFAGWVHQVAGNGTTINLFMDYETFGEHQWEDSGIFSFLKELPGRILRHPDFCFR
                     TVTQVSRAHQPVGTIDAHSPISWADIERDLSAWMGNSMQKEAIRGIYELEEAVHATGD
                     PALLDIFRKLQTSDHFYYMSTKYWSDGDVHKYFSPYGTPHDAYIYYMNALHDFRERLG
                     M"
     gene            6090..7223
                     /locus_tag="PKK76_06520"
     CDS             6090..7223
                     /locus_tag="PKK76_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68159.1"
                     /translation="MLFTEGKETTVAKADIALFREDEYGTKSYRDMLAAREEARAKEL
                     KEIESRLDEVRAFSPTGLWLGAGYGQGYLATPQEAALVGDRATLNLVPRGGQAAAYFP
                     LHGHSAKGGDAHIRFDADRFYVEAIGFSYEARGSISAIGLPPVTAAFNPGSITFNQWW
                     TIGVFARHQNASGSVGIEPYRFSKRLRAFVFAGKSGFNTMTIADGPLFSSATILPSLY
                     FTALVQRFKTRLSGSGPRGGLELRFQAFTRLEFRASAAAASYAGITKDRGTLVIGPST
                     IPGVSGGSLIMQHGSLWVRKYDYSLGGYFAVNSRLRVFVIGSDEESRIIFRNRIPQNY
                     LRDLNLSNYYSQDGFGEFLLRYYGRPTYTGRFRKITAGAEFRI"
     gene            7382..>7663
                     /locus_tag="PKK76_06525"
     CDS             7382..>7663
                     /locus_tag="PKK76_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HNL68160.1"
                     /translation="MNQKHQKLYELLERTQRFDKESLFRAFAHHVEYRIGKYKDNINM
                     RDVYQSVAYTIRDTLIDRWNETEEIFRKEKAKRIYYLSMEFLLGRLLETS"
BASE COUNT         1999 a         1994 c         1852 g         1818 t
ORIGIN      
        1 aggatgcgat ccaactgcga gatccgcata caggcaaacg ccgagatcca gacatgcacc
       61 ggaaacatct gaaaactggc ggtcgaacaa atactggaga aatatataga attgaactct
      121 tctgtggctt gcatctgcaa atgcgcgcac cgcatcactc gatgcgtcct gaaaagcttc
      181 tggccatccg tgaaagcctg ccggcctgtc cagattgtaa tcaaaccttt cctccagaca
      241 atcgaagatc gcctgacggg agagttctat gccaccctcc tccacgtatt cggcaaagtc
      301 ttccggagga tctgaaatga attcgtcgaa gagaatctca aagaaccgca tcttccattg
      361 gtacacgccg gcgtagtcaa tgcgctcctc tggcctggtc cgggcaagat acagttgaaa
      421 ttctgaactt tcaaacaagc tgcgtgcctt tgcagatgct gcgaattcag gaaggagcgg
      481 aatagaaacg aacatggcat ttttgtagag tctgctcgaa ggagaataag gacttttgaa
      541 atccggtcgc atgaggaacg gagcatgcag cggggaaagg ccaaggattc ggatgccgcg
      601 gggcccaacc agacgcgcga gttcgagcaa atctcccaga tccccaattc cccagtttgc
      661 gctcgaccga agtgcgtaga gctgcagcca cagaccattc catctggatc cattttcgat
      721 ctctgcattc aagtagcagg attccggaca cagaaacaaa gtcgatcgcc ctatctcttc
      781 tttccatcgc ataacactga ttatgtgctg tcccggtgga atagaatcct tgagatctac
      841 ttcgtataga ccaatcgtgc gaccggccac aagatgctct tcttttaact caaactgcgg
      901 tctgaggcca actgtgattc caccttcgag ttcaatgcgc agctcaatct ggcgaactat
      961 gtccaccgga atccgcatgt tgaatctggg cgcatagacg gaggcaatga cggcgggtgc
     1021 gatgagagtg gtggcatctg cttgaatcag gtctgaaagg attgcagatg gatctccgct
     1081 gtaacccagg atctcaagaa tggttcgctt tgcttcttct gaagttgtgt gagcgactcc
     1141 gtcaatgtca tgatactcaa gcgaaatccc cgctcgcctg gacagaatat caagtgcaga
     1201 ctctgcaggc tgcatttcaa cctcttcgat ccggtctaaa gacagaaact gtaagagcca
     1261 tcaaatccag agacgttcca gccttcacag aaatggaagg accaagctca gagtcgagga
     1321 tcctgtacca tgaaaggttg ggctccgcgg gtatccgaaa ctcgcctgcg ctgcgcgcga
     1381 tgacaatcaa gagaggttcc acatcttcat ctttgccacc tatttcgtag aacttcttgt
     1441 tctcctgcgg atgaagacca tcaatgatcg caccaaaaat gggcatggaa tcataccatt
     1501 ctgaactctt catttcgtgc ccgtccggat gaagccagat catgtctctc tcttctcctg
     1561 gttctcctcc gcgaatgaaa tgattcctgc gaagcactct actctgtctg cgtaaatgga
     1621 tgagtttctt tacgaattcc agatgatgct tttgttcttc tgtcagatcc cagtcaatcc
     1681 aggaaatgtc attgtcctgg caatacgcgt tgttgtttcc tttttgagtg cgggccagct
     1741 cgtctcccat gttgatcatg ggtactccct gcgaaagtag tagagttgca aagtagtttc
     1801 tcatctggcg aaagcgagta gcgcgaatgg ttggatcctg cgtctctcct tctgtgccgt
     1861 gattgaaact gatgttgtga tcgtttccgt ccgtgttatt ccagccgttt gcttcgttgt
     1921 gttttctatc atagctgacc agatcgagca tggtgaatcc gtcatgacag gtaacaaagt
     1981 taatacttgc atacggacgc cgaccggccg cttcgtatag atcgctggac cctgtaagac
     2041 gagtggcaaa ttcgcgcgct acattcccac caatccagaa actgcgcaca cagtcgcgat
     2101 agtgtccgtt ccactccgtc cattgaacag gaaaattgcc cacctggtag ccgccttctc
     2161 ccagatccca gggctcagcg atcagcttca ctctagacag aactggatcc tgatgaatga
     2221 tatcaaagaa tgcagacagt ctgtccacag catggagctc tcgaccaagc gcgcttgcca
     2281 gatcaaatcg aaaaccgtcc acatgcattt ctgtgaccca gtagcgcaga gaatccatga
     2341 tgagctgcaa cacccgcgga ttctgcatgt tgagagtatt tccacaacca gtgaagtctg
     2401 tgtacatccc ggggttgttt ggatcgagtc gatagtaaga agcgttatca atgccgcgaa
     2461 agcaaaggtt ggcccctctg tgatcccctt cgcaggtatg attgtatact acatccagga
     2521 tcacttccag ccctgctcta tgcaacgctt tgaccatgac tttaaattcc tggatggcat
     2581 cccccactct ccttccagcg gagtacgcaa gatctggagc aaagtaagag agagtgctgt
     2641 agccccagag gtttttcagt ccgcgcatgg ccagcacatg ttcatctaca tggtggtgaa
     2701 ccggcatcag ctcgatagcg gtgattccaa gactcttgaa atactctatc attggttctg
     2761 aggcaagacc tgaataggtc ccacgcatcc tctgcgggac ctccgggtga cgcatcgtca
     2821 gtgatttcac gtgagcttcg tagatgatcg tatcgcgcca cggaattctg ggaggtcgat
     2881 cgtctcccca ggtgaatgct ttttccacaa ccgcacagag agctgcaaat ggtgcgctgt
     2941 ccctctcgtc catcctggac ggattgtttg gatcaaacgc atacaaagaa tcatcccaca
     3001 aaacactgcg ggccattgca aaagcataag gatctgccag caatttgtta ggattaaacc
     3061 gatgaccctt cgcaggttcc cacggtccgt acactctgta tgcgtagatc tgccccggcc
     3121 gtacgtctgg aaggtagcag tgaaagatca tatctgtgcg gtgcgtcata gaaattctac
     3181 gactctcttt cttcgcatca gcggaatcga atagacacag ctctacgccg gttccatgtt
     3241 cggagtacag agagaaattt acgccagatc catcccaggt tgctccgagt ggataaggct
     3301 caccggccat gacttttatc accgtacact cctttgaaaa agcatggaat tcatccccat
     3361 tctatgataa ttgttgtaaa catcaaatag aaaacgaaaa ggggttctat atgctgcgca
     3421 ccgggcctgg aatcggctaa tgagcaacta caagatcttc atgattggct gggagtatcc
     3481 cccccgaatc acgggaggac tggccgtcgc atgcgaagga atcgcatcgt ctctgggaag
     3541 acgagggaac agtgtgcgct ttctcgtacc cagaatgcat ggagacgaac cagttcatga
     3601 aaatgtagaa ctcgtagatg cgggtacaag aacagaactc tatactacag acgaactgga
     3661 agtgcttcgc agcatggcgc tgcatgattt cacaaccaga caggtctttt ccccctacgc
     3721 atcctcggag tattcaggcc aatctctggt ggacatcaca gaaaagctcg cggtcatgca
     3781 aaaacaatcc cgcgaaatcc ccctccttcg cggaggatac ggggactgga tgtaccagga
     3841 aatcgagcga tacgcacgcg ccgcgtactt catcgcacaa aaaacagaaa tggatgtcat
     3901 tcacgctcat gactggatga cctttcctgc gggtctcgcg tgtatgaatg ctactggagc
     3961 tcctctggtc gttcacgtgc acgccactga gtttgacaga agcggagaaa gtgtaaacca
     4021 gtacgtatac gacctcgaaa gatatacatt tcatcatgca actgccatca tgactgtgag
     4081 caattacaca aagaatgtgc tggtgcaaag atacggcgtt tcgccggaaa aaatatttcc
     4141 cattcacaat gcagttgagt ttgaactccc gggcgcggtc ctcgaaagaa agaaagcact
     4201 caaggatcac atcgttttgt ttctgggtag aatcacattc cagaagggac cggattactt
     4261 cgttcgtgca gcgcaactcg tgcttcagca aatccagaat gtgcgatttg taatggcagg
     4321 cacaggagac atgtattcac gcatgatcga atatgcagca gacctgggga ttggtaagtt
     4381 cttccactac actggattca tgaatcgcgc gcaggtaaag agactctact ccatgagcga
     4441 tctttacgtg atgccatctg tatccgaacc atttggaatc tctcctctag aagcaatgat
     4501 gcacggcgtt ccagtcatcg tatccaggca gtcaggtgta agcgaagtca ttcaaaattg
     4561 catcaaagta gatttctgga acgtagaaga actcgcagaa caaatccttc gagtcctgca
     4621 cgacacggaa ctcaagcaaa acatgcagca agaaggccat agagaagtac aggcaatttc
     4681 ctgggacgct cccgctgaaa agatggagca ggtgtacggg agacttgtga aatgatcgca
     4741 gtgtgcttct atttcgaagt acaccagcca ttcagacttc gcccctacca cttcttcgat
     4801 gtgggcgtgg atcctcacta tctggatgaa caaaaaaacc gcgatatatg ccgcaaagtg
     4861 gcaaacaagt gctatcttcc tgcaaacaaa gtactcctgg atctaatcaa gctctacggt
     4921 tctgatttcc gtgtttcatt ctccatcaca ggaactgcga tcgaacagtt cagaatgtat
     4981 tcgcccgaga ccctggatag ctttcgcaaa ctcgcagata ctggacaggt tgagtttcta
     5041 aacgaaactt actatcacac tctcgcatct ctgtattctg aaaacgaatt tcgcgctcag
     5101 gtggcaaagc atggccagct catgcgcgaa gaattcggat acaatccagt tgcatttcga
     5161 aacacagaac tcatctactc caatcacatt gcgtggcttg cgcgcgaaat gggatttcag
     5221 accatccttc tcgaaggaat cgaacgagtg ctcgactgga gaagcccaaa tcatgtgtac
     5281 gcgcctgagc acagtcctgg aatcaaatgc cttctcaaga attatagact cagcgatgat
     5341 gttgcgtttc gattttcaga cagaaactgg aaagactggc ctttgaccgc agagaagttc
     5401 gcaggctggg ttcaccaggt ggcaggcaac gggactacga tcaatctctt catggactat
     5461 gaaaccttcg gagaacatca gtgggaggat tcggggatct tcagttttct caaagaattg
     5521 ccaggaagaa ttctcagaca tcctgacttc tgctttcgca ctgtgaccca ggtttcaagg
     5581 gcacaccagc cggtaggaac cattgacgct cattcaccta tttcgtgggc agacatagaa
     5641 cgcgatctat ccgcgtggat gggaaacagc atgcaaaagg aagccattcg tggcatctac
     5701 gagttggagg aagcggtcca tgcaacggga gacccggccc tgctcgacat attccggaag
     5761 ttgcagacct cggaccattt ctattatatg agcacaaaat actggagcga tggggatgtt
     5821 cacaagtact tcagccccta tggcactccc cacgatgcat atatttacta tatgaatgct
     5881 cttcacgatt ttagagaaag actgggcatg tagtccgggc ataccccgag ccgacaagta
     5941 attgagttcg tctaattctt ccggaggttg atctgcgctt tctcatcgcc attctactgc
     6001 tttccagttc gcttgcggca gacacaatcc gcctcaaaga cggcaccctt ctgactggaa
     6061 aagtcacgtc tcaggatcct tctgtgattg tgctcttcac ggagggcaag gaaaccacag
     6121 ttgccaaagc agacattgct cttttcagag aagatgaata cggcacaaaa tcctacaggg
     6181 atatgctcgc cgccagagaa gaagcacgcg ccaaagaact caaagaaata gaatcacgcc
     6241 tggacgaagt gcgcgcattc agtccaacgg ggctatggct cggtgcaggc tacggacagg
     6301 gatacctggc cactccccag gaagccgcac tcgtcgggga cagagcgact ctcaatctgg
     6361 ttccgcgggg cggccaggcg gcagcttact ttccactcca tggtcattct gcgaaaggag
     6421 gagacgctca cattcggttc gatgcggatc gcttttatgt ggaggcaatc ggtttttctt
     6481 acgaggcaag aggcagcatc tcagcgattg gacttcctcc cgtaaccgcg gcattcaacc
     6541 ctggaagcat tacattcaat caatggtgga cgatcggagt gttcgccaga caccagaatg
     6601 catctggctc cgtcgggatc gagccgtatc gattctctaa aagacttcgc gccttcgtat
     6661 ttgcgggcaa gagtggtttc aataccatga ccatcgcaga tggtccactc ttctcttcgg
     6721 caacgatcct tccatctctc tatttcacgg cacttgtcca gagattcaag acaagactat
     6781 ccggctcagg tccccgtggt ggtctggagc tgcgctttca ggcgttcact cgactcgaat
     6841 tccgcgcgag tgctgcggcg gcatcttacg ctggcataac gaaggatcgc ggaactctcg
     6901 tgattggccc gtcgacaatc cctggtgtaa gcggcggctc tctcatcatg cagcatgggt
     6961 ctttgtgggt cagaaagtac gactattctc tgggaggtta ctttgcggtg aactccaggc
     7021 tacgcgtctt tgtcattgga tccgacgaag aaagccggat catctttcgc aatcgcatcc
     7081 cgcaaaacta cctgcgagat ctcaacctga gcaattatta ctcccaggat ggatttggtg
     7141 aattcctcct gcgttattat ggccgcccca cttacacagg acgttttcgc aagatcacag
     7201 cgggggcgga gttcagaatt taggcccgga gtaaatcctc ccggaactcc cttgcaattg
     7261 cggctggcct ttttgaacga aatgacgcgc acgataggtg gaaaaaaacc agaaaggatg
     7321 ctaaaaatct ttgacgacct attgaaccga tccagattcc cggcaaaccg tcgggggagc
     7381 aatgaatcag aaacatcaga aactatacga acttcttgag cgcacgcagc gattcgacaa
     7441 ggaatccctt ttccgcgcct tcgcgcacca cgtagaatac cgcattggaa aatacaaaga
     7501 caacatcaac atgcgcgacg tttaccagtc agtcgcctat acaatccgcg atactttgat
     7561 cgaccgctgg aacgaaacag aagaaatctt ccgcaaagaa aaagcaaaaa gaatctatta
     7621 cctctccatg gaatttctac tcggacgact tctcgagaca tcc
//
LOCUS       DAORFF010000120         7524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4874_length_7524_cov_3.512117, whole genome shotgun sequence.
ACCESSION   DAORFF010000120 DAORFF010000000
VERSION     DAORFF010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7524
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4874_length_7524_cov_3.512117"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..743
                     /locus_tag="PKK76_06530"
     CDS             81..743
                     /locus_tag="PKK76_06530"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNL68161.1"
                     /translation="MKTKRAVLTISIFSLLGGACLTPQPTRYENVDERVEVTRAPAGT
                     VRKKIALVNFRNMTDKSYLVQPATIQLTSLMVQSGYFEIIEPTLVESVIRAQDEITTE
                     KLQTLKDKFGAEYFLTGNLTNFEIRETSAGFCLFLGLLGSKNTKEYIVETGIDYRLVS
                     VPDAKILQADSVENRRTDTSSQAGFLFSTGGSDVKVLQSSGGKLLRYAMKDLVEKLIR
                     GL"
     gene            751..1650
                     /locus_tag="PKK76_06535"
     CDS             751..1650
                     /locus_tag="PKK76_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68162.1"
                     /translation="MRAIAPLLLFVSCAGIIQKQESLESRTAYIAPRAGFEKIKVVLL
                     PFQNSARMGEFDIIPDGDYSSKVIRRTQNTITSDTLVPLTLGDAPPQEPEGILPGDLY
                     REIAETELFQSGRFEVIPYYKFAEERAKIDPEKKDPTSVLKTAQSMGIQLLISGDCTD
                     FEIKQSRSFWKIPLWAIILAASFAIDDRNLRAVIWEAMIRMLFVVPLNSPFWDEGIGW
                     EDTDLDVLVSLNMRASGPSGSIRFSDESAVKRTESVRNLDLLVSKESRKVRIQKASAG
                     RQIRFATSDLVRKISREADQGLR"
     gene            1676..2449
                     /locus_tag="PKK76_06540"
     CDS             1676..2449
                     /locus_tag="PKK76_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNL68163.1"
                     /translation="METVLVTGGASGIGRAIAQEYIAKGARVILADKNRALLQSTARS
                     LGCDWVEMDVTSSSSIKKARANVIKKVGGVDVLVNNAGVVFGGRFEDVSVERHSLTYQ
                     VNTLGPVLVTHAFYPDLLSRPGSKIVFVASASGYIGLPRGTTYASSKWSIVGFAESLR
                     AEVEETGRGPSVTLVCPSYVDTGLFAGAKAPLLTPLLHAGDVGRRIVDAAERGRPVLN
                     MPWIVNIIPWIRSVLPRFLYKAILRITGVTRSMDDWHGR"
     gene            2548..5061
                     /locus_tag="PKK76_06545"
     CDS             2548..5061
                     /locus_tag="PKK76_06545"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL68164.1"
                     /translation="MARYLLCLLLVLTAACNRGDLIPLGGTWDFKKGFDQKDLTDPSR
                     SGWDTVKLPASITETKNLQGYAGWITLRKELPPESADILSRGAAAINMGWTSDVSYYY
                     INGQLIGQVGSAQPYETGLFRRFIKEIPQVPAKEKNYLVIAVYGTGEYPLQFDTDAYF
                     GPGESVFNEYFQNEVVSFFLLGIYFVVGAYHLLLFSRRPQDKHNLLFGIFCTLLTLYW
                     LNRTGSRDPLFGSHVIARLRIEYISLFFIPPSLMFFLSQFFYRRFSRVGLAVFAVCLV
                     LAVITLFAPYTVAKNCLTVWQYSSIVAIGYAIFYILREAFKKNQDARWLALGVIVLMI
                     AALHDIAAARHWINSTHIARYAFLIFVLAIAAILANRFMRVHTEVEQLNEKLEDKVRV
                     RTQQLTESLDEVQKLKIQQDGDYFLTSLLIQPLTGNFVKSQNVSVDMLLRQKKQFQFR
                     HWQAEIGGDLCIAQSISLRRRTYTVFLNGDAMGKSIQGAGGALVLGTVFKSMVSRTQL
                     AEAAQNKAPEQWLKECFVELQNVFVSFDGSMMVSAVFGLVDEESGMLYYINAEHPFVV
                     LYREKKAEFLENDMQLRKLGVSGLGGHLEVKTYQLLPNDVVIIGSDGRDDVQIGVDRL
                     GNRIINENEMEFLRHVEKGDSDLRRIEKSIMGFGQLTDDFTMLRIGFKEAAAQQRSAD
                     ADQRIAAFKAEVRKARGLFNDGATDEAIFVLEQAMDMTEENADAMRLLVKIMIQAGMF
                     DRAANLSDSYTRRFPADTEFLYITSYAYAKSGETRQAIEFAERCRLRDPSMVRNLTHL
                     ARLHAQTGNEPLARRVLGEALQVDPLHAKARQMLAAIED"
     gene            5061..6047
                     /locus_tag="PKK76_06550"
     CDS             5061..6047
                     /locus_tag="PKK76_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL68165.1"
                     /translation="MRWILIPAVCFCCSFQTLRAQEVEDNANQDFPDLFAEGLAPQSY
                     PGGSFERARHDFVLCRGKTGKGDPPPGRPEEQVEGLSAGAYNNAVIDDVIRDIDATDA
                     GSAFRKKHLTIALEKMKIGLKVDPQFFPFLYNSGRLLLLLNLPEQALSYLLLARARLS
                     EFSAVHLFIGAAHARLGDGISATESYRVAYRRNPFDPSALVALGDFYLESGAFPQAHD
                     TYERVLKQFPENASARIGLGRLYAKKGEFVRARTFFESVVTENQDGSERSDYDRALHF
                     YFAEVCFSLKDYREAVKQYDRILKHPEDPFFLDNSLTLMKKRREIARKLAEE"
     gene            complement(6054..>7524)
                     /locus_tag="PKK76_06555"
     CDS             complement(6054..>7524)
                     /locus_tag="PKK76_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68166.1"
                     /translation="WPAVIGTFLVGAGYEDRHADKVAKRSQSDGLGSIFATPLVLGTY
                     SLWTNGGFLLIAQDQYKNIRNQRAEYRRTIQNRNILLAGALATFGMAVIDGALMSDSQ
                     GFFYTTPGPGVGPIPGTRNLSNSTFTWKFTKDVWIPGKVRRNAGDTAGTVTLGIFGAL
                     GAVTLYESYHQYQLVRTSRRLKDSVANQAVTNPILGVALASYWTNPGFVYAVFDNNRQ
                     FTSLRNQYRRSTQVQVAAVGTAAFLYIFQVLDAASYSSSPGFFGPAPFEPGTNPPPMA
                     DMYCFGGPCVYSVTSNFRMRASAFIPGRTRIQNGDKKAGYMIAGTMIGFGAAAVFEDY
                     EANKARRAAIRAGNSGVNALFSSQLAVAAGYQYWTSSSYLFLYQQYYHDVRKQSRAYS
                     RAVNARTTMLGGAAILYGLQFLDATTLPASNASISTEKQIAFSEAATFTQANVASHLL
                     GDLKQDLRREAVHGLVQDRIEFKDRFPYDVAEFHKTFAF"
BASE COUNT         1781 a         1810 c         2018 g         1915 t
ORIGIN      
        1 ttattctggg ctatttcatc accgcagtat tctatcggtg aactttgctc tcattttctc
       61 gtctaatatt aagagggtgt atgaaaacga aacgcgcggt gcttacaatt tccatctttt
      121 ctcttctcgg aggcgcttgc ctgacacctc agcctactcg ctacgaaaac gtggatgaaa
      181 gagtagaagt aacccgggct cctgcaggca ctgtgcgcaa gaagattgcc ctggtcaact
      241 ttagaaacat gacggacaag tcctatctgg ttcaacctgc taccatacaa ctgacgagtc
      301 tcatggttca gtcggggtac tttgaaatca tcgaacccac tctggtagaa agcgtgattc
      361 gcgcgcagga tgagatcaca acggagaagt tgcaaactct gaaagacaaa ttcggcgcgg
      421 aatacttcct tactggaaac ctcaccaact ttgagattcg ggagacttca gccgggttct
      481 gtttgtttct tggtttgctc gggtcaaaga atactaaaga atatatagta gaaacaggta
      541 ttgactacag gctcgtttct gttccagatg cgaaaatact tcaggcagat tccgttgaga
      601 atcggcgtac ggatacgtcc agccaggcgg gtttcctttt ttcaaccggc gggtctgatg
      661 tgaaggtgct ccaatcttct ggtggaaaat tgttgcgtta cgccatgaag gacctggtcg
      721 aaaaactcat tcgcgggttg tagtatgaaa atgcgggcga ttgcgccgct gcttctgttt
      781 gtttcctgtg caggcatcat tcagaagcaa gaatctcttg aatcgcggac tgcctatatc
      841 gcacccaggg caggctttga gaaaatcaaa gtagttcttt tgccttttca aaattcagct
      901 cgcatgggtg agttcgatat cataccggat ggagactaca gttcgaaagt cattcgtcgc
      961 acacaaaaca caatcacatc agatacgctt gttccgctca ccctggggga cgcacctccc
     1021 caagaaccgg agggcattct cccaggagat ctttaccgtg aaatagcgga aacagaactc
     1081 tttcaatctg gaagattcga agtgatccct tactacaaat tcgcagaaga acgtgcgaag
     1141 attgatccgg aaaagaagga tcctacgtcc gtcttgaaga ctgcgcagtc gatgggcatt
     1201 cagcttctga tttccggtga ttgcactgat tttgaaatca agcaatccag gagtttttgg
     1261 aagattcctc tctgggcgat catactggct gccagctttg ccattgatga tcgaaatttg
     1321 cgtgcagtga tctgggaagc aatgattcgc atgctcttcg tcgttccgct caattctcct
     1381 ttctgggatg aggggattgg ctgggaagac acggatctgg acgtccttgt atctctaaac
     1441 atgcgcgcga gcggaccttc tggcagcatt cgattttcgg atgagtctgc cgtgaagcgg
     1501 acagaaagcg tgcggaatct ggatcttctt gtctcgaaag aatcgcgaaa ggttcgaatt
     1561 cagaaagcat ctgcaggcag acaaattcga ttcgcaacat ctgatctggt tcgcaaaatc
     1621 tcccgcgaag cggatcaagg cttgcgctga aatccatcat tctgattttt tgttcatgga
     1681 gacagttctg gttacggggg gcgcaagcgg gatcgggcgt gccatcgcgc aggagtacat
     1741 cgcaaagggc gctcgagtca ttcttgcgga caagaatcgc gctctccttc aatccaccgc
     1801 acgcagcctg ggatgtgact gggttgagat ggatgttacc agttcctcat ccataaagaa
     1861 agcccgcgcg aatgtgatca aaaaagtcgg tggggttgat gtactggtca acaatgctgg
     1921 agtggtcttt ggcggacgtt tcgaagatgt aagtgtagag cgtcattctc tcacgtatca
     1981 ggtcaatact ctgggccctg tgctggtgac tcacgctttc tatccggatt tgctttcgcg
     2041 gccaggaagc aagatcgtgt ttgttgcaag cgcatcgggc tacattggac tgcccagagg
     2101 aaccacctac gcgtcgagta aatggtctat cgttggcttc gcggagtctc tccgcgctga
     2161 agttgaggag acggggaggg ggccttctgt taccctcgta tgcccgtctt acgtagatac
     2221 tggcctcttc gcaggcgcga aggcacccct tttgacgccg ttgctgcatg caggggacgt
     2281 gggccgacgt attgtagacg cggcggagag gggtcggcca gtactcaaca tgccatggat
     2341 cgtcaatatc atcccctgga taagatctgt tcttccgcga tttctgtata aggcaatact
     2401 tcgcataacc ggtgttacgc gaagtatgga cgactggcac ggtcgctgag cgggaagcga
     2461 tcctgggcga aattggggta tttggcgtcc cggcggagcc gtctaagaat ctcttgacct
     2521 gacctggctc gaagggacga tttacccgtg gcgcgttatc tactctgcct gctccttgtc
     2581 ctcaccgcgg cctgcaatcg aggggacctg attcctctcg ggggaacctg ggattttaag
     2641 aagggctttg accaaaagga tctaacggat ccgtcccgct ctggctggga cacagtgaag
     2701 cttcctgcaa gcattacaga gacaaagaat ctgcagggtt atgcaggctg gatcactctt
     2761 cgcaaagaac ttccacccga atccgctgac attctatccc gtggcgccgc cgctatcaat
     2821 atgggttgga cttctgatgt ttcgtattac tatatcaatg gccagctcat tggccaggtg
     2881 ggaagcgcgc agccgtacga gaccggattg ttcagacgct tcatcaagga aattccgcaa
     2941 gtcccggcaa aagagaagaa ctacctggtc atcgctgtat acgggaccgg agagtatccg
     3001 cttcaatttg acacggacgc atatttcgga ccgggggagt ccgttttcaa tgaatacttt
     3061 cagaacgaag tggtttcctt ctttctattg ggaatctact tcgtcgtggg cgcgtatcat
     3121 cttttattgt tttcgcgcag accccaggat aagcacaatt tactgtttgg aattttctgt
     3181 actcttctga ctttatactg gctcaatcga acgggatctc gtgatccatt gtttggctct
     3241 cacgtgatcg ctcgtctacg catcgaatat ataagtttgt tcttcattcc gcccagcctg
     3301 atgttctttc tttctcagtt cttctaccgc cgtttttcgc gcgtaggtct ggcggttttt
     3361 gcagtatgtc ttgttcttgc ggtcatcacc ctttttgccc cgtatacggt tgcgaagaat
     3421 tgtctcaccg tctggcagta cagctctatc gtggcgattg gatacgctat cttctatatt
     3481 cttcgcgagg ctttcaaaaa gaatcaggac gcgcgctggc ttgctctggg cgtcatcgtt
     3541 ctcatgatcg cagctcttca tgacattgct gctgcaaggc actggatcaa tagtactcac
     3601 atcgcacgtt atgcgttctt gatctttgtg ctggccattg ctgccattct tgccaatcgc
     3661 tttatgcgcg tgcatacgga agtggagcaa ctgaacgaaa agctggaaga caaggttcgg
     3721 gttcgcacac agcaattgac cgaatcactc gatgaagtgc agaagctaaa aatccaacag
     3781 gacggggatt atttccttac ttccctgctg attcaaccac tcacgggaaa ctttgtgaaa
     3841 agtcagaatg taagtgtgga tatgcttttg cgtcagaaga aacagtttca gtttcgccac
     3901 tggcaggcgg aaattggcgg ggatctgtgc atcgcgcaga gcatcagcct caggcgtcgc
     3961 acctatactg tctttttgaa cggggatgca atgggaaaat ccattcaggg tgcaggtggc
     4021 gctctggttc tgggaactgt atttaagtct atggtttcgc gtacgcagct tgcggaagcg
     4081 gcgcagaaca aagcccccga gcaatggctc aaggaatgct ttgtagagct tcagaatgtt
     4141 ttcgtttctt ttgatggtag catgatggtt tcggctgtat tcggtctcgt ggacgaagag
     4201 agcgggatgc tgtattacat caatgcggag cacccgtttg tagttctata cagagagaaa
     4261 aaagccgagt tccttgagaa tgacatgcaa ctgcgaaagc tcggcgtttc aggcctgggc
     4321 ggtcatctgg aagtgaaaac gtatcagctc ttgcccaatg acgtagtgat cattggatct
     4381 gacggccgcg atgacgtgca gatcggcgta gatagactgg gaaatagaat catcaatgaa
     4441 aacgaaatgg aattccttcg ccatgtggaa aagggcgaca gtgatctgag acggattgag
     4501 aagtcgatca tgggttttgg ccagctcaca gacgatttca caatgttgcg aattggcttc
     4561 aaggaggcgg cggcgcagca gagaagcgca gacgcggacc agaggattgc ggcgttcaag
     4621 gcggaagtga gaaaagctcg cgggctattc aacgatggcg cgacggatga agccatattc
     4681 gtgctcgagc aggcaatgga catgacagaa gaaaacgcag acgcgatgcg attgcttgtg
     4741 aagataatga ttcaggcagg aatgtttgat cgtgcggcca acctttcgga cagttacacc
     4801 agacgattcc cggcggacac agaattcctc tatattactt cgtacgccta tgcgaagagt
     4861 ggggaaacca gacaggccat cgagtttgcg gaacggtgtc gtcttcgcga tccatccatg
     4921 gtgagaaatc taactcatct cgcgcgactt cacgcgcaaa caggaaatga gcctcttgcg
     4981 cgtcgggttc tcggggaggc cctgcaggtg gatcctcttc atgcaaaggc gcgccagatg
     5041 ctcgctgcga ttgaggattg atgcgctgga ttctaatccc cgcagtttgc ttttgttgtt
     5101 cgtttcaaac ccttcgcgcg caggaagtag aagacaatgc gaatcaggat ttccctgatc
     5161 tgtttgcgga aggtcttgcg cctcagagtt atccgggtgg cagctttgaa agagcaaggc
     5221 atgactttgt gctttgccgc gggaaaacag ggaagggaga tccgcctccc ggaaggccgg
     5281 aagaacaggt agaaggattg tctgcgggtg cgtataacaa cgctgtcatt gatgacgtga
     5341 ttcgagacat tgacgcgacg gatgcgggaa gcgccttcag aaagaaacac ctgacgatcg
     5401 cacttgaaaa gatgaagatc gggctcaaag tcgatcctca attttttcca tttttgtaca
     5461 actccggcag actcttgctt cttttgaatc ttccagagca ggcgctttcc tatttacttc
     5521 ttgcgcgagc gcgactttcc gaattttctg cggtgcatct tttcattggt gccgcgcatg
     5581 cgagactcgg agatgggatt tctgcgactg aaagttacag agtcgcctat agaagaaatc
     5641 cctttgatcc atcggcgctc gtcgcactcg gcgattttta tctcgaatca ggcgcttttc
     5701 cgcaggccca cgatacttac gaaagagtcc tcaagcagtt tccagaaaac gcaagcgcgc
     5761 gcattggact cgggcgattg tatgcgaaga agggcgagtt tgttcgcgcc agaacgttct
     5821 ttgaaagcgt tgtcactgaa aaccaggacg gttcggaacg ttcagactat gacagggctc
     5881 tgcatttcta tttcgcagaa gtatgttttt ctctgaaaga ttatcgcgaa gcagtcaaac
     5941 agtatgatag gatcctgaaa catccggagg atccattctt tctggacaat tccctcacgc
     6001 tgatgaagaa gagacgcgag attgcgcgaa agctggcaga agaataggcg aagttaaaac
     6061 gcgaacgtct tgtggaattc cgccacatcg tacgggaatc gatccttgaa ttcaattcga
     6121 tcttgcacga gtccatgaac cgcttcgcgg cgcagatcct gtttcaagtc gcccagcaga
     6181 tgagacgcga cattcgcttg cgtaaacgtg gctgcttctg agaaagcgat ttgtttttca
     6241 gtagaaatgg aggcattact tgctggcaat gtggtagcat ccaggaattg aagcccgtat
     6301 aaaatggccg ctccaccaag catcgtagtt cttgcattta ccgcccgtga gtacgctcga
     6361 ctttgctttc gcacatcgtg gtagtactgc tgatagagga acaagtaaga tgaagaagtc
     6421 cagtactggt atccggccgc gactgcaagc tgcgaggaaa agagagcatt cactccgcta
     6481 ttgcctgctc tgatcgccgc gcgacgcgcc ttgttcgctt cgtagtcttc aaaaactgcg
     6541 gcggcaccga atccgatcat ggttccagcg atcatgtatc ccgctttctt gtctccgttc
     6601 tggatccgag ttcttcccgg aatgaacgcg gaagcgcgca tgcgaaagtt ggatgtaaca
     6661 gaataaacgc acggccctcc aaagcaatac atgtctgcca tgggaggagg gttcgttcct
     6721 ggttcaaagg gggctggtcc gaagaaacct ggtgaagacg agtaggaagc cgcgtccagt
     6781 acctggaaaa tgtacagaaa agctgccgtg ccgacggctg ctacctgcac ctgcgtgctc
     6841 cgacggtatt gatttcgcag cgaagtgaat tgtctgttgt tatcaaagac cgcatagacg
     6901 aatccagggt tggtccaata ggaggcgagc gccacaccca gaattggatt tgtaaccgct
     6961 tgatttgcaa cgctgtcttt tagtctgcgc gaagtgcgca ccagctggta ctgatgatag
     7021 ctttcataca gcgtcacggc accgagcgcc ccgaagattc cgagagtaac ggttcccgca
     7081 gtgtctcctg cgtttctacg tacttttccc ggaatccaaa catcttttgt gaatttccac
     7141 gtgaatgtag agttagagag gttgcgcgtc ccggggattg ggcccactcc tggaccgggc
     7201 gtagtgtaga agaatccttg agagtcagac ataagcgcgc cgtctatgac cgccatgcca
     7261 aacgttgcga gagctcctgc caggagaata ttgcgattct ggatggttct tctatattcc
     7321 gctctctggt tgcgaatgtt cttgtattga tcctgcgcga tgagaaggaa gcctccgttc
     7381 gtccagagag aatatgttcc aagcaccaga ggcgtggcga agatgcttcc cagtccgtca
     7441 gattgcgatc gcttcgctac tttgtccgca tgtctatctt catagcctgc tcccaccaga
     7501 aacgttccga tgactgcagg ccag
//
LOCUS       DAORFF010000121         7516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4891_length_7516_cov_4.218067, whole genome shotgun sequence.
ACCESSION   DAORFF010000121 DAORFF010000000
VERSION     DAORFF010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4891_length_7516_cov_4.218067"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..890)
                     /locus_tag="PKK76_06560"
     CDS             complement(60..890)
                     /locus_tag="PKK76_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68167.1"
                     /translation="MFKPLYLLIAFISLANCESILSRRPTIDFVDADVTDLTMKYIDL
                     RLKLKLNNPYPIKLPPGALGGDLSLDGKTITGITTTTPEVAASGAGTFPLQIRIPFES
                     IMNLGQDLEKKEEFHFRVKGNADVQVTALAGAPAARLNLPFDYEQNVPAFVPDITFGN
                     LQLETPSMASPSAGVSFDLGVRNKAAGKFNLGKMDFQLLSNGMSLLNGSTQEVVNSGT
                     ETKARIVSKVSLLAVPFALLQGSKNFTVKGGTTLSFPGFFGGKTFPLQFEKPLQESAR
                     "
     gene            complement(883..1980)
                     /gene="fliG"
                     /locus_tag="PKK76_06565"
     CDS             complement(883..1980)
                     /gene="fliG"
                     /locus_tag="PKK76_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNL68168.1"
                     /translation="MQKRSIDVLISDEGILPTNPVPQMKRPAQTPVPPTSDSGATRAA
                     KLLLSLGPDQASVILREMEPAEVEKVVSEMIRIRSITATEKKSILGEFHAIVEDFDPP
                     VRGGIDSATDLLVRSLGEQKAGEILSRVNRRDVRNDFAFLEGIDPNLLSTVLSAEHPQ
                     VVAIALASIHPRTAASVLRSFPDELRAAVSLRIAKTSKTHPDALEKVARVLREKFEKR
                     KDEIYSEVGGADTLATILNHMDRDLEDNILKELETAAPDLLTTVKEKLYTFEELIALT
                     PKEMRLLASQINDDSILACALRGAGEDMRRMFFNNLSQNRAADILEEIDRRGPISLRE
                     IHEARGFILSAARRLDDEGRIVIKKSKEEYV"
     gene            2091..4622
                     /locus_tag="PKK76_06570"
     CDS             2091..4622
                     /locus_tag="PKK76_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNL68169.1"
                     /translation="MNGIPGLPEILAPAGGREQFFAALGAGADAVYLGLKGFNARARA
                     ENFTLEDLRELVPLARTSGMRVLVATNVVLKQDELPLIMDAIAELDWIGVHALIIQDL
                     GLARTIRERMPWMRMHASTQLAVHNEDGAAFAADMGFKRVVLARELTARDISRIKEAV
                     GDRIELEVFCHGSLCYSYSGLCFFSGAEDARSGNRGECAYTCRKPYRIVSEPGYGFLF
                     SMKDLATSDHVDALLGAGVSALKIEGRKKDAQYVASSVGLYKQARDRALGEASGAARN
                     FSEDLKSSYQRDTTSFFVKGRYHENVIDLDNAGHRGTRIGVVQSAGKGWMEVTTSEPL
                     DLFDGLRVDSPDQVYHALPQQGQNLRGSVKKAQAKYENEICQFSLREMQKGSKRMTTA
                     LAGDHLRIQIPSDARVKEGDVVFRVRSNELKRYVDSISSESGRLRSLYLMRMLVSIQD
                     GPPLQLRASLFDHSGEIVSASIQTEASRPRGTSTLAQDLRETFALLGDAGVECDVEVE
                     GDPEWFVPRSKIKELKRMLSSQALQSMPAILAARKRDARPSSQGSAPVARATTYQIKV
                     DRLEYLSALDQILTSHPGLVDEIIFEPKKSNISKSSPTDVFASLRSVADKHSVHVRFA
                     IPVVIRSWDEGHLRAYVNEYVLQGGSRFEVANVGAFKFLQDHKIALTDLASDFTLYAL
                     NSQSVLSLHELGCNRVALSVEDDWQNLQRLLAQWPHQEILPQAILYKDTPLFIAEACS
                     LTALHNGCPTSKVCGYRTLEIENDEGDRFFVAHEQCKSIVFGKNAYSISGRRSLLEGA
                     GVHRFRLDFLTRLYSKEDILRIANLAFKDQQIANTHPANFHRKLL"
     gene            4659..4952
                     /locus_tag="PKK76_06575"
     CDS             4659..4952
                     /locus_tag="PKK76_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68170.1"
                     /translation="MNLLMRIFILLSLLWMIGCKDLRDGYEKGFKEEFQKSFTKSCAS
                     SAVKKGMNEAKATEYCACTAKELASRHSSQELMKMAASDKDGEVAAAVKACTK"
     gene            complement(4933..5478)
                     /locus_tag="PKK76_06580"
     CDS             complement(4933..5478)
                     /locus_tag="PKK76_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68171.1"
                     /translation="MAEMMLRRTRADQAARVYAKFTSRFTTAQSAQANREEVLAILAP
                     LGLKWRTKQILETIDYLKDTYKTRKPSANDDLTRIPGVGEYSNAMIRNRLYGERLPAL
                     DSNMARLICRISGKKFTQESRRNRFVSDVAHSLVQTKHSRDLNLAVLDYCALVCKPTR
                     PLCGQCKLISKCKYGATSYRP"
     gene            complement(5567..6658)
                     /gene="dcm"
                     /locus_tag="PKK76_06585"
     CDS             complement(5567..6658)
                     /gene="dcm"
                     /locus_tag="PKK76_06585"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HNL68172.1"
                     /translation="MQKPKVPGSRSVSTDHTSMRPQSRGIARANNRRNYKIIDLFAGI
                     GGIRLAFENAGYSSVFSSEWDKSAAFTYAANFKDTPAGDIRAVDSRTLPDFDVLCGGF
                     PCQPFSIAGVSKKNALGRPHGFADETQGTLFFEVLRILKDKKPSALLLENVKNLTSHD
                     KGRTMQVILAALKEAGYHVHHSLLNARKLLPQNRERIFIAGFRNLQSFQAFQFPELKD
                     RSPRLSDILEKNVPAKYTLSDHLWQYLQDYKAKHEAQGHGFGFGLASRDGISRTLSAR
                     YYKDGSEILIPSRGKNPRRLTPRECARLMGFPDHFKIVVSDTQAYKQFGNSVAVPVVQ
                     AIAENMKKALDAATKTQRTSAHGRIRISV"
     gene            complement(6698..>7516)
                     /locus_tag="PKK76_06590"
     CDS             complement(6698..>7516)
                     /locus_tag="PKK76_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68173.1"
                     /translation="NVDRIEFRGWNAIAIANNVYQITIPIDIGIRILGLSHNGDSNWL
                     HVSEEDAGKKGEEAWRLYGGHRLWIAPEHPELSYESDNDPITWSQTENGILLIQKPGK
                     WTGLEKQMQISFSEKGINLLHRIINRSHAPVKPSLWPITALRGEGRATLPVACDTGPG
                     LNPAGSVILWPYTRMNDQRISWDASSLCIEHKPSSALKMGVSRTGDSCRLTYNRQGKT
                     FSKEFRTPHGSYPDLGCVAQCYTNENLIELESLSPLTDVPPGSALEHLETWSIS"
BASE COUNT         1748 a         1799 c         2002 g         1967 t
ORIGIN      
        1 ttggtcagag atgcgtccct gtgtcgcggg acgcaagcat ttagcgagaa ccgccagact
       61 taacgggcgc tttcttgcag tggcttttca aactgcaacg gaaatgtttt ccctccgaag
      121 aaaccgggaa aactcagagt ggtaccaccc ttcacggtga aatttttact tccctgcaac
      181 agggcgaaag gcactgcaag aagggaaact tttgaaacaa tcctggcttt tgtttctgtt
      241 ccggagttca ctacttcttg agtcgatccg ttgagcagtg acatgccatt ggagagcaat
      301 tgaaagtcca tttttcccag attgaatttc ccggcggcct tgtttcgaac tccgagatca
      361 aaactgacgc ccgcggaagg agaggccata gacggcgttt ccagttgcaa atttccaaac
      421 gtgatatctg gcacaaatgc gggtacgttt tgctcgtagt caaacggaag gttcaatctc
      481 gcagcaggag cgccggcgag tgccgtgacc tggacatctg cgtttccttt tacgcgaaag
      541 tgaaactctt cctttttctc caggtcctgg cccagattca tgatcgattc aaaaggaatt
      601 ctgatttgca gcggaaatgt gccggcgccc gaggccgcta cttccggagt ggtggtagtg
      661 atgcctgtga ttgtttttcc atcgagagaa agatctcctc ccagagcgcc cggaggtagt
      721 ttgattggat atggattgtt taacttcagt ttcaatctca gatcaatgta tttcatcgtg
      781 agatcggtta catccgcatc cacgaaatca atggttggcc tacgactgag gatgctttcg
      841 cagttggcaa gcgagatgaa ggcaatcagg aggtagagag gtttaaacat attcttcctt
      901 tgacttcttg ataacaatgc gtccttcgtc gtcaagtcgt cttgctgcag aaagaatgaa
      961 tcctcgggcc tcgtggattt ctctgagtga aatcggaccc cgccggtcaa tttcttccag
     1021 tatgtctgcg gcccggttct gcgagagatt gttgaagaac attcgcctca tgtcttctcc
     1081 agcgcctctc aaagcgcatg ccaggataga atcgtcattg atctgcgatg cgagaagtct
     1141 catttctttt ggagtgagag caatgagttc ttcaaacgtg tagagtttct ctttcacagt
     1201 ggtgagcagg tcgggtgcgg ctgtctcgag ttcttttaga atattgtctt ccagatcgcg
     1261 atccatgtga ttgagaatcg tcgccagcgt atctgcgcca cccacctctg aatagatttc
     1321 gtcttttctc ttttcaaatt tctcacgaag cacgcgcgcc actttctcca gtgcgtccgg
     1381 gtgagtcttg ctcgtcttgg cgatgcgcaa tgatactgca gcacgcagtt catccggaaa
     1441 agaacgtaga acggatgctg ctgttctggg atgaatgctc gccagtgcaa tcgctacgac
     1501 ctgcgggtgt tcagcagaaa gaactgtgga aagcaggttt ggatctattc cttcgagaaa
     1561 agcgaaatcg tttctaacat ctcgacgatt gacgcgtgaa agaatctctc ctgccttttg
     1621 ttcgcccagg ctgcgcacga gtagatccgt ggcgctgtct attcctccgc gaacaggtgg
     1681 atcaaagtct tctacaattg catggaattc acccaggata cttttctttt ccgtcgccgt
     1741 gatgcttcgg attcggatca tttccgagac cactttttcc acttctgcag gttccatctc
     1801 gcgaaggatg acagacgcct ggtccggtcc gagagacaac agtagcttgg ccgcgcgcgt
     1861 ggccccggag tctgaagtag gaggcaccgg cgtttgcgcc gggcgcttca tctggggcac
     1921 agggttcgtc gggaggatgc cctcatctga aatcagaacg tcgatgcttc tcttctgcat
     1981 gactcactca tgtgacataa tccagaggag gcaagttgat tttgcagcga aactgcgatt
     2041 gtctattttt tctgatcgcc ccggggcgtg attctgctgt cttgattggt atgaacggga
     2101 ttcctggatt gccagagatt ctggcacctg cggggggcag agaacagttc tttgccgcgc
     2161 ttggcgcagg tgcagacgca gtgtatctgg gactcaaagg attcaacgcc agagcacgcg
     2221 ctgaaaattt cactctggaa gatctgcgcg aactcgtgcc tctcgcgcgc acttcaggaa
     2281 tgagagtgct cgtggccacg aacgtggttc tcaagcagga tgaacttccg ctgatcatgg
     2341 acgccatagc ggagctggat tggattggag tgcatgcttt gatcattcag gacctcgggc
     2401 tggcgcgtac cattcgcgaa agaatgccat ggatgagaat gcacgcgtcc acgcagcttg
     2461 cagtacacaa cgaagacggc gctgcctttg ccgcagatat gggattcaaa cgagtcgttc
     2521 ttgctcgtga actcactgcg cgagatatct cgcgaatcaa agaagcagtg ggcgatcgga
     2581 ttgaactgga agtgttctgt cacgggagtc tgtgttacag ttacagtggt ctttgttttt
     2641 tcagcggtgc agaggatgcg cgaagcggca atcgaggcga gtgcgcatac acgtgcagaa
     2701 agccatatcg aattgtgagc gaaccaggat atggattttt gttcagcatg aaagatctgg
     2761 ccacgagcga tcacgtggat gctcttctgg gtgcaggtgt gagtgccttg aaaatagaag
     2821 gcagaaagaa agatgcgcaa tacgtagctt cttcggtcgg tctttataaa caggcgcgcg
     2881 atcgagctct tggcgaggct tccggcgctg cacgcaattt cagcgaagat ttaaaatcaa
     2941 gctaccagag agacaccacg agcttctttg tcaaaggacg ataccacgaa aacgtaatcg
     3001 atctggataa cgcgggtcat cgcggaacca ggattggtgt ggttcagagt gcaggcaaag
     3061 gctggatgga agtgaccacg agcgaaccat tggacctttt tgatggctta agagtggatt
     3121 caccggatca ggtttatcac gccctgccgc agcagggaca gaacttgcgt ggatctgtaa
     3181 aaaaggcgca ggctaaatat gaaaatgaaa tatgccagtt ttcattgcgc gaaatgcaga
     3241 aaggatcgaa gagaatgacc acagctctcg cgggagatca tcttcgcatc cagattccat
     3301 cagatgcgcg tgtcaaagaa ggagacgttg tatttcgagt gcgctcgaat gagctcaaaa
     3361 gatacgtgga ttccatttct tctgaatcag gacgattgcg atctctctat ctcatgcgca
     3421 tgctcgtttc tattcaggac ggtcctccgc tgcagcttcg ggcctccctg ttcgatcatt
     3481 caggagagat cgtttctgct tctatacaga cagaagcaag tcggcccagg ggcacttcta
     3541 ccctggcgca ggacctgcgg gaaacctttg cactgttagg tgatgcagga gttgagtgcg
     3601 atgtggaggt cgagggggat ccagaatggt ttgttccgcg cagcaaaata aaagaattga
     3661 agcgaatgct ttcttcgcag gccctccaga gcatgcccgc gattctcgcc gcgcgaaaga
     3721 gagatgcgag gccttccagt caagggagcg ctccggtggc tcgcgcgaca acatatcaga
     3781 tcaaggtgga tcgcctggaa tatttgtccg ctctggatca aattctaacc agtcatcctg
     3841 gtctggtgga tgaaatcatc ttcgagccaa agaaatcgaa tatttcaaaa tcttcgccga
     3901 cagatgtctt cgcatctctt cgaagcgtag cagacaagca tagcgttcat gttcgatttg
     3961 ccattccggt tgtgattcga tcgtgggatg aaggccatct gcgcgcctat gtgaatgaat
     4021 atgttctcca gggagggtct cgatttgaag tcgccaatgt gggtgcgttt aagttcctcc
     4081 aggatcacaa gatagcgctg accgatcttg cctccgactt cactctgtac gctctcaata
     4141 gccaatcggt tctttccttg catgagcttg ggtgcaatcg agtggcgctc tctgtcgagg
     4201 atgactggca gaacctgcag cgccttctag cgcaatggcc ccaccaggag atactccctc
     4261 aggcgatttt gtacaaagac acaccccttt ttattgcaga ggcttgctcg cttacggctc
     4321 ttcacaacgg ttgccctact tcgaaagtct gcgggtaccg cactctcgag attgaaaacg
     4381 acgagggaga tcgcttcttt gtagcgcatg aacagtgcaa atccattgtt tttggaaaga
     4441 atgcgtatag catttccggc agacgctcct tgctcgaagg ggccggtgtc catcgcttca
     4501 ggttggattt tttgacccgg ctctattcca aagaagacat actcaggatt gcaaatctag
     4561 cattcaagga ccagcagatt gcaaacaccc atccagcaaa ttttcaccgc aaattgcttt
     4621 gaccctggaa aagaatttgt aattccaaat agaacgttgt gaatctgctc atgcgtattt
     4681 tcattttgct ttctctgctg tggatgattg gatgcaaaga ccttcgcgat ggttacgaga
     4741 aaggcttcaa agaagaattc cagaagagct tcacaaaatc ctgtgcgtca agcgcggtga
     4801 agaagggaat gaacgaagct aaggcaaccg aatactgtgc atgcaccgcc aaagaactcg
     4861 ccagtcggca cagttcgcag gaattgatga agatggctgc ctctgacaag gacggagaag
     4921 tggcggcggc tgttaaggcc tgtacgaagt agcgccgtat ttgcattttg aaatgagctt
     4981 gcattgaccg cagagtggtc tggtgggctt gcaaaccaga gcgcagtagt ccagcaccgc
     5041 caggttcaaa tcgcgcgagt gttttgtttg taccagagaa tgcgccacat cggatacaaa
     5101 tcgatttctt ctggattctt gcgtgaactt tttgccagag atgcggcaaa tcaggcgtgc
     5161 catatttgag tcgagagcag gtagtctttc accgtaaagc cggttgcgaa tcattgcatt
     5221 tgaatattca cctacacctg gaatcctcgt gagatcgtcg ttcgcggaag gctttcgcgt
     5281 tttgtatgta tcctttaagt aatcgatggt ctcaagaatc tgtttcgttc tccatttcag
     5341 accaagcgga gcgagaatag cgagaacttc ttccctgttt gcttgagctg attgggccgt
     5401 tgtgaatctg gaagtgaact tagcatacac tctggctgcc tggtctgcgc gcgtacggcg
     5461 cagcatcatt tctgccatgg tgagatggaa ccagttccca gtttttctcc agggatagtc
     5521 tctggcgttt ttcttgaacc aggccaggag agtacggcgg acggatctat acggaaattc
     5581 ggatgcgtcc atgggcggaa gttcgctgcg tttttgtcgc ggcgtccagc gcctttttca
     5641 tgttttcagc aatggcctgg accacgggga ctgccactga gtttccgaat tgtttgtagg
     5701 cctgggtgtc cgagactaca atcttgaaat ggtcagggaa acccatgagt ctggcacatt
     5761 ctctgggagt gagtctcctg ggattttttc ctcgcgaagg aatcagaatt tcagatccgt
     5821 ctttgtagta acgtgcagat agagttcttg aaatcccatc gcgcgacgcc aggccaaatc
     5881 cgaaaccgtg tccctgcgct tcgtgcttcg ctttgtagtc ttgcaggtat tgccagaggt
     5941 gatcggacag agtgtatttg gccggtacgt ttttctccag aatgtctgat agccttgggg
     6001 accgatcctt cagttccgga aattgaaatg cttgaaatga ttgcagattg cgaaacccgg
     6061 cgatgaagat tctttctcga ttttgcggaa gtagttttcg agcgttgaga agggagtgat
     6121 gcacatggta gccagcctct ttcagtgcgg ccagaatcac ttgcatcgtt cttcctttgt
     6181 catgcgacgt caggttcttt acattttcta gcagaagagc ggacggcttc ttgtctttga
     6241 ggatgcgcaa tacttcaaag aagagagtgc cctgcgtttc atcggcgaag ccatgcggtc
     6301 tgcccagggc attctttttc gaaacccctg caatggaaaa gggctggcat ggaaacccac
     6361 cgcagagcac atcaaagtcc ggcaaagttc tgctatctac tgcgcgaatg tcccctgccg
     6421 gcgtgtcctt aaaattggct gcgtaagtga atgcggctga tttgtcccat tcactcgaaa
     6481 aaacagacga gtagcctgcg ttttcaaacg ccagacggat tccgccgatg cccgcaaaca
     6541 gatcgatgat tttgtagttt cttctattgt tagcgcgcgc aatgccgcgg gattgtggcc
     6601 gcatagatgt atgatcggta gaaacggacc tggagcctgg aactttaggt ttctgcatgc
     6661 ttctgtctac agcctgagtg tgacaggttt cgagaaatca cgagatcgac catgtttcca
     6721 ggtgttctag tgcagagccc ggtggcacat ctgtaagagg agaaagcgac tctaactcga
     6781 tgaggttttc gtttgtgtaa cactgtgcca cacatcccag gtccgggtag ctgccgtgtg
     6841 gcgtgcggaa ttccttggag aatgtttttc cctgacgatt gtatgtgagc ctgcaggaat
     6901 caccggttcg cgaaactccc atcttcagag cgctcgacgg tttgtgctca atacaaaggg
     6961 agcttgcatc ccaggaaatt ctctgatcgt tcattctagt gtaaggccag aggatcacac
     7021 tacccgcagg gttgagtccg gggccagtgt cgcacgctac aggaagagtg gccctgcctt
     7081 ctccacgaag tgcagtgatg ggccacagcg aaggcttcac aggtgcgtgc gaacgattga
     7141 tgattctgtg aagcaggttg atccccttct ctgaaaatga aatctgcatt tgtttttcca
     7201 gaccggtcca tttcccaggc ttttgaatca aaaggattcc gttttcagtt tggctccatg
     7261 tgattggatc attgtctgat tcgtaggaaa gctctggatg ctccggagca atccagagtc
     7321 tgtgaccgcc atacaatctc caggcttctt ctcccttttt gcctgcatcc tcttcagaaa
     7381 catggagcca gtttgagtcc ccattgtgtg aaaggcccag aattctgatt ccaatatcta
     7441 ttgggatagt gatttgatag acgttattcg ctattgcaat ggcattccat ccgcgaaact
     7501 cgatcctatc cacatt
//
LOCUS       DAORFF010000122         7497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4905_length_7497_cov_4.292663, whole genome shotgun sequence.
ACCESSION   DAORFF010000122 DAORFF010000000
VERSION     DAORFF010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7497
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4905_length_7497_cov_4.292663"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKK76_06595"
     CDS             <1..213
                     /locus_tag="PKK76_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68174.1"
                     /translation="VLPFLVGQPGRVLADEVAASVARKLAEALPRIDGFRGLGKDGAG
                     SDRDKAAMWILREWKSLCPLRNAIRP"
     gene            complement(323..910)
                     /locus_tag="PKK76_06600"
     CDS             complement(323..910)
                     /locus_tag="PKK76_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HNL68175.1"
                     /translation="MKNLLKHDGVAVYHGVVFPSETSDAYFGSLLSEIEWKNDEAIMF
                     GRHIITARKVAWYANQAFSYTYSRKTRHALPWSPALRELKQIVEDRTGYTFNSCLCNL
                     YHNGNEGVTWHSDDEKSLKKHAAIASLSFGAERVFQFKHKKSGDIVSILLEHGSLLIM
                     SGATQTNWKHRLPISKKILEPRINLTFRTMEHQAT"
     gene            complement(1015..1887)
                     /locus_tag="PKK76_06605"
     CDS             complement(1015..1887)
                     /locus_tag="PKK76_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNL68176.1"
                     /translation="MIRLISGLILTLFVAACSTPGQARFEPQGAQTVAKTKTVIFIHG
                     MFMTPASWANWQKYFESRGYKTLAPAWPYHEGKPSDQRASHPNAQLGKLTLDDVLESY
                     RKVIREQKEKPILIGHSMGGLIVQLLMQEGLGAAGIAIDSAPPKGLISLKFSFLTSNW
                     GVISPFANKEEPLLLSPEQFNYAFVHTLTESEQKSVYDSFVVPESRLVGNGPTTDTAA
                     IDFSKGRLPLLMIAGELDHITPASMNYSNFQKYESSPSKTEFRMFNGRTHWIAGQKGW
                     EEVADFVLEWINRN"
     gene            complement(1982..3574)
                     /locus_tag="PKK76_06610"
     CDS             complement(1982..3574)
                     /locus_tag="PKK76_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNL68177.1"
                     /translation="MKKIVCNPRRPSRFWVTLLDQTQLKLPEVIAALSLATDLANDNP
                     PETTLRICYIALEIAREAGLRPRELEDLYYGALLRFIGCTAYAHEEAVAMGDDVAARH
                     SLARVDVSRPSRVLGTALRSSKGKSKPAVLWNFLTQSGTMFSNFTAANCEVACMLGQK
                     IGMPEGVLRVLEQIHERWDGKGGPSRVRGNALLASVRILHVANTAEIIRQAAGTDAAC
                     AVIQDRSGEQFDPDVCKGFLRIGRDLLHSMETVSVWDEVIRRAPRSESSPGGARLRKI
                     AEAFGSFVDLKSVYTAGRSLNISNTVERAAQWMGLKDTEDLVIASLLADIGMVSVPTN
                     LLEKKGKLNPSEWERVRLHTYYTERILTRSPVLARIAPIAGAHHERLDASGYHRGSSG
                     PSIPQGGKLLAAADLYHALLERRSYRAPVAPSSAAQMLREEVARGRIDRTCADAVLAV
                     TNHTAISSSPGAESTSTLSPRECEVLTLLASGFSNKEIGAKLNISHRTVQHHVEHIYG
                     KISVSTRAAATLYAVQNGFLRA"
     gene            3745..4047
                     /locus_tag="PKK76_06615"
     CDS             3745..4047
                     /locus_tag="PKK76_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNL68178.1"
                     /translation="MVGLLRNKPLSISDISRPFRMSFAGVAKHVDVLEGAGIVRKVRD
                     PEDRRVFIIQLQREPLDEAVDWLEYHREFWSGKLDSLEAFMEEQDGNADRKNRKKN"
     gene            4016..4438
                     /locus_tag="PKK76_06620"
     CDS             4016..4438
                     /locus_tag="PKK76_06620"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNL68179.1"
                     /translation="MQIVKIEKRIKADCKRLFRAWLDPSDFSRWFLPGEKVGIGNVTL
                     DPQPGGYFKIDMMVNGQVRPHEGSYVEITEPSKLVFTWKSWKTGGRDTLVTITFVPEG
                     DTTLITLIHEQLPDAEACESHTHGWTGILTGLAAFFEK"
     gene            4457..4924
                     /locus_tag="PKK76_06625"
     CDS             4457..4924
                     /locus_tag="PKK76_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNL68180.1"
                     /translation="MNRIYHKVGIQATAEKIFNALTTQSGLAGWWTNEVTGAFSGGHS
                     RPGETIRFGFGSLGSMEMEVDELSRERKVRWECKKGPEDWIGSHIEFTLEKSSTNDQM
                     TIVYFNHQDWKKESEFMGHCSMKWAVFLLSLKSLIEKGEGNPSPNDVKIDDWN"
     gene            4996..5151
                     /locus_tag="PKK76_06630"
     CDS             4996..5151
                     /locus_tag="PKK76_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68181.1"
                     /translation="MHPVDSIRNLKKLYQVRIGFEFKDGRPDEESRQRYRTEWKKVLP
                     PDCDLSY"
     gene            complement(5148..5933)
                     /locus_tag="PKK76_06635"
     CDS             complement(5148..5933)
                     /locus_tag="PKK76_06635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNL68182.1"
                     /translation="MRARSLLIFLFGLTCVGLTCAIDIATQDSIRFGAFYMVPITFVA
                     WREGRVAGIVVALFASACYAYANSPFDKMLVANSALECGIYLSNAVVIGLLRARFHES
                     RWLANHDPVTGAFNQRAFERSANAMLSNIGSPGIGMAFLDADHFKQVNDTHGHQKGTD
                     VLIRIAECIQEHVAPGCTVARYGGDEFVIMQNGITKEEWVALVRRIVADVARTMKTDG
                     LPVTLSAGATHIVRSASLEELMHECDLLMYRAKSQGRNRVESD"
     gene            complement(5938..6342)
                     /locus_tag="PKK76_06640"
     CDS             complement(5938..6342)
                     /locus_tag="PKK76_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNL68183.1"
                     /translation="MCKTIKQKVKFKTDPETLYELLSNSKMHSRITGQKAVISEKIGG
                     SFETLSGKVTGINVDLLRGKRIVQAWRRSDFPAGIFSMAAVTFEKTTDGGTQLVLTHR
                     GVPKDLIAGIEDSWRNHYWKRIKSYLDEQDRN"
     gene            complement(6434..7297)
                     /locus_tag="PKK76_06645"
     CDS             complement(6434..7297)
                     /locus_tag="PKK76_06645"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNL68184.1"
                     /translation="MKTDKSLKRSIECTIKDFGFPFLLMTYTMTLWFFPQSATFLLLA
                     FAVAGMALEKWIPYHAEWQGIKTDSKIDAIFSGLTILVSVILKSGTTVVLSRAGMESH
                     VWPSWPLPISVLVALVISGLGPYLLHRAAHEWSAIMWQVHAVHHAPDRVYSLNAMRTH
                     PLNAAWNVLAGLGPLLLLGADSQTILIAGSLNNFFSIFNHMNIDFRNPMLSIFFNTSE
                     LHRWHHSTKQELGDKNYSSGAIALWDHVFGTWRLPAERFKSNGAGLFAANDFPGYSLS
                     KQLLRPICRCA"
BASE COUNT         1788 a         1868 c         1984 g         1857 t
ORIGIN      
        1 gtcctcccgt tccttgtcgg tcagccaggc cgggtactcg cagacgaagt cgcggcctcc
       61 gtcgcgcgca agctcgcgga agcactgccc aggatcgacg ggtttcgagg gctcggcaaa
      121 gatggagccg gctccgaccg cgacaaggcc gcgatgtgga tcctgcggga gtggaaatcg
      181 ttgtgtcctc tgcggaacgc gatccgtcct taaatctgcg tgagcatgcc cgggtcatcc
      241 atcagaagat gagggcagga gccagacatc cagcaggaga gctgccgcgt cgtcagaaag
      301 cacgttaggc gattttgatt cctcacgtag cttgatgttc catagtgcga aatgtcaggt
      361 tgatcctggg ctccaggatt ttctttgaaa ttggcaatcg gtgtttccaa tttgtctggg
      421 tggcaccgct catgatcaaa agacttccgt gctccagcag aatcgagaca atgtcccctg
      481 atttcttgtg tttgaattga aataccctct ctgcgccaaa gctgagagat gcaatcgcag
      541 cgtgtttctt taacgacttt tcatcgtcgc tgtgccaagt gactccttcg ttgccgttgt
      601 gatacagatt gcacaggcag gaattgaatg tgtaaccagt tcgatcctct acaatttgtt
      661 ttaattcgcg gagcgccggt gaccagggca gagcatggcg agtctttcgt gaatatgtat
      721 acgagaatgc ctggttcgcg taccaggcga ctttgcgtgc tgtgatgatg tgccggccaa
      781 acatgatggc ttcatcgttc ttccattcaa tttcagacag aagcgagcca aaatatgcat
      841 cgctcgtctc tgatggaaaa accacaccat gatagacggc cacaccatcg tgcttgagta
      901 aattcttcat tcttcacgcg atttctgaac tacagggctt cctgcggatc accattctct
      961 ctttcgggtt tggttccgca ggtcttgtcg ccctaccggc cagcaaacat accgttagtt
     1021 gcgattgatc cattcgagga cgaaatcggc aacttcttcc cagcctttct ggcccgcgat
     1081 ccagtgtgtg cggccgttga acattctgaa ttcagttttg gaaggcgagc tttcatattt
     1141 ctgaaaatta gaatagttca tggatgcagg cgtaatgtga tcgagttctc ctgcaatcat
     1201 cagtagaggc agcctacctt tcgagaaatc gatggcagcc gtgtctgtcg tcgggccatt
     1261 ccccaccagt ctggattccg gtacgacgaa tgagtcatac accgatttct gttctgactc
     1321 agtcaacgtg tgaacgaacg catagttaaa ttgctccggc gacagaagaa gcggctcttc
     1381 tttgttcgca aatggactga tcacacccca gttgcttgtc aaaaacgaaa atttcagcga
     1441 tatcagccct ttaggtggag cagagtcgat cgctatgccc gcagcgccca ggccttcttg
     1501 catcagcagc tggacaatca gtccacccat ggaatgtcca atgaggattg gcttttcctt
     1561 ttgctcgcga atcacttttc tgtaactttc cagaacatcg tctaacgtca gttttccgag
     1621 ttgcgcattg ggatgcgacg ctcgctgatc agaaggtttt ccctcgtgat aaggccacgc
     1681 tggcgcgagc gtcttgtagc ctctggattc aaaatacttt tgccagttag cccagctggc
     1741 gggcgtcatg aacatcccgt gtatgaaaat caccgttttg gtctttgcaa ctgtttgtgc
     1801 gccctgaggc tcaaaacgag cctggcctgg cgtgctgcag gctgctacga atagggtcag
     1861 gatcaatcct gatatcaatc taatcatttg tttcctccgc gaaactacca gaatactatc
     1921 acaactgaag agggcgcgca tcggccattt ggcctatgct gggcaagcgg accatttctg
     1981 atcaggcgcg caggaatccg ttctgcacag cgtagagagt ggcggctgcc ctggtggaga
     2041 cagagatttt gccgtatatg tgttccacgt ggtgttgcac ggttctgtgt gaaatattca
     2101 atttcgcgcc gatctctttg tttgagaatc cgctggccag gagagtcagc acttcgcatt
     2161 cgcgcgggct gagcgtggat gttgattcgg cgccaggaga tgaggaaatg gccgtgtgat
     2221 tggtcactgc gagcaccgca tcggcgcatg tcctgtctat tctgcctctc gccacttctt
     2281 cgcgcagcat ttgtgcagcg gaggacggag caacgggcgc gcggtaagat cgacgttcca
     2341 aaagggcatg atacaaatct gccgctgcca gcaacttgcc gccttgcggg atggagggac
     2401 cagaacttcc tcgatgatat ccggaggcat ccaatctttc atgatgcgct cctgcaatcg
     2461 gtgcgatgcg agcaagcaca ggggagcgtg tcagaattct ctccgtatag taggtatgca
     2521 gacgcacgcg ttcccactcg gaaggattga gtttcccttt cttctcaagc aagttcgtgg
     2581 gcacactgac catgccgatg tctgcgagta ggcttgcaat gaccaggtct tccgtatcct
     2641 tgagacccat ccattgcgcg gcccgttcga ccgtgttact gatgttaagc gagcggcctg
     2701 ccgtatagac ggacttgaga tctacaaaac tgccaaacgc ctctgcaatt ttgcgcagcc
     2761 gagcacctcc gggtgagctt tcactccgag gggcccttcg aatcacctcg tcccagacgg
     2821 aaacagtttc catagaatgc aacaaatcac gaccaattcg caggaaacct ttacaaacgt
     2881 ccggatcaaa ttgctcccca cttctgtcct ggatgacggc acaggccgca tctgttcctg
     2941 ctgcctgtcg tatgatctct gcggtgtttg cgacgtgcag gattcgcacg gatgccagaa
     3001 gcgcattgcc gcgcactcta gacggtccgc cttttccatc ccatcgttcg tgaatctgct
     3061 cgaggacacg caacaccccc tccggcattc cgattttttg ccccagcatg cacgcaactt
     3121 cacaattggc tgcggtaaaa ttggagaaca tagttccgga ttgggtgaga aagttccaga
     3181 gcactgcagg tttcgacttt cccttgctgc ttcgcaaagc agtccccagt acccgagaag
     3241 gccgactaac gtctactctg gccagggaat gtcgcgctgc gacatcatcg cccatggcga
     3301 ctgcttcctc atgggcgtat gcagtgcaac cgatgaacct gagcaaggca ccgtagtaca
     3361 ggtcttccag ctcacgaggc ctgagaccag cttctcgggc gatttccagg gcaatgtagc
     3421 aaatccgcag agtggtttcg ggtggattgt cgtttgccag atccgttgcc agagacagtg
     3481 cagcgatgac ttcaggtagt tttagttgag tctggtccag caaagtgacc cagaaacgcg
     3541 acggccgtcg gggattgcac acaatttttt tcacgaaagg cgatcgtctt tattcaacca
     3601 aatggttttg tagttgtcat ttgcggggtg tcctatttat taaaccagat ggttgaatta
     3661 aaaagaaagg tgcgcccggt gcggtccgcg caacgaaatt tggactccgt gttttccgcc
     3721 ctcgcggatc catcgcggag aaaaatggtg gggctgctgc gcaataaacc gctgagcatt
     3781 tctgacattt ccaggccatt tcgcatgtcg tttgccggag ttgccaagca cgtggatgtg
     3841 ctcgaaggtg cgggaattgt tcgcaaggtt cgagatcccg aggaccgccg agtcttcata
     3901 attcaactgc agagagaacc gctcgatgag gccgtagact ggctggaata tcaccgagag
     3961 ttctggtccg gtaagctgga cagtctggaa gcattcatgg aggaacagga tggaaatgca
     4021 gatcgtaaaa atagaaaaaa gaattaaagc ggattgtaag cggcttttta gagcgtggct
     4081 ggatccgtcg gacttttccc gttggttttt accaggagaa aaggtcggaa tcgggaacgt
     4141 gacgctcgat ccgcaacccg gcggatattt caaaatcgat atgatggtca acggtcaggt
     4201 ccgcccgcac gaaggaagtt atgtggaaat cacggaacct tcaaagctag tctttacatg
     4261 gaaatcgtgg aagacaggcg gaagggacac tctcgtgacc atcaccttcg tgcccgaagg
     4321 cgacactaca ttgatcactc tcatacacga acagctgccg gacgcggaag cctgcgaatc
     4381 tcatacgcac ggttggacag gaatcctcac cgggctcgca gcatttttcg aaaaataaga
     4441 tcaaaggaag gaagctatga accggatata tcacaaggtt ggcattcaag ccacagcgga
     4501 gaagattttc aatgctctga ctacacagtc aggtctggca ggttggtgga cgaatgaagt
     4561 gacaggcgct ttctccgggg gccattcgcg ccccggagaa acgattcgct ttggctttgg
     4621 ttcactgggc agcatggaga tggaagtaga cgaattgtct agagaacgca aggttcgctg
     4681 ggaatgcaaa aaaggtcccg aagattggat tggatcgcac attgagttca ccctcgagaa
     4741 gtcatcgacg aatgatcaga tgacaattgt atatttcaat catcaggatt ggaaaaagga
     4801 aagtgagttc atgggtcact gcagtatgaa gtgggccgtg ttcttgttga gtttaaaaag
     4861 tctgatcgag aaaggagagg ggaatccttc tcccaatgac gtgaagattg acgactggaa
     4921 ctaagtctgc cgacggcagc gcgcatacta cgtgcgcgct gcgttctgga tctcagaaaa
     4981 agaaacggac cgccggtgca tccggtcgat tccattcgca atctaaagaa actatatcaa
     5041 gtgagaatcg gctttgaatt caaagacgga agaccggatg aggagagtcg tcagcgatac
     5101 cgcacggaat ggaagaaagt tctgccgccg gattgcgacc tttcttacta atcagactct
     5161 actcgatttc tcccctgaga cttcgcgcga tacatgagaa gatcgcattc atgcatgagt
     5221 tcttccagag aagcgctgcg cacgatgtga gttgccccgg cgctcaaggt tactggaagg
     5281 ccatccgttt tcatggttcg ggcaacgtcc gcgacgattc tccgcaccag ggcgacccat
     5341 tcttcctttg tgattccgtt ttgcatgatc acgaattcat cgccgccgta gcgggccacg
     5401 gtgcagccgg gcgcaacgtg ttcctgaatg cactctgcaa tgcgaatcaa tacgtccgtt
     5461 cccttctggt gtccatgcgt gtcattgacc tgcttgaaat gatctgcgtc cagaaacgcc
     5521 atgccaattc ctggtgaacc gatgttcgag agcatggcat tcgcgcttct ttcgaatgca
     5581 cgttgattga atgcgcctgt gactggatca tgatttgcaa gccacctgct ttcgtggaat
     5641 cgggcacgca gtaagccaat aacaactgca ttgctgagat agatcccgca ctcaagagcg
     5701 gaattcgcaa cgagcatttt gtcgaacgga gaattggcgt atgcgtagca agcagatgca
     5761 aacagtgcaa cgacgatccc ggcgacgcga ccttctctcc atgctacaaa agtgatagga
     5821 accatgtaga atgcgccgaa tcgaatggag tcctgggttg caatgtcgat ggcgcaggta
     5881 aggcccacgc acgtgaggcc aaaaagaaaa atgaggagcg atcgggcgcg catggactca
     5941 attgcgatct tgttcgtcca ggtaggactt gattctcttc cagtaatgat tacgccagct
     6001 gtcttctatt ccggcaatga gatcctttgg gacgccgcga tgagttagca cgagttgcgt
     6061 tcctccgtct gtcgtttttt caaacgtgac ggccgccatg gaaaaaatcc ctgcagggaa
     6121 atctgatctt ctccatgcct ggacgattct ttttcccctc aatagatcta cattgattcc
     6181 ggtaactttg ccggacagag tttcgaatga gccgccgatt ttttcgctga tgactgcctt
     6241 ttgccctgtg attctcgagt gcatcttaga attagaaagc aattcgtaaa gggtttccgg
     6301 gtctgttttg aatttgactt tttgcttaat cgttttgcac ataggaatct tcttcaatta
     6361 caagaatcgt gcgaaattgg caaacgattt atgcccacgg tcatttggct cgaagttcga
     6421 gccgttttcc gcgtcaggcg catctgcaga tagggcgcag tagttgtttg gacaaagagt
     6481 atcccggaaa atcatttgct gcaaacaacc cggcaccatt gctcttaaat ctctctgcgg
     6541 gaagtctcca ggtaccaaaa acatggtccc acagtgcgat cgctccggag ctgtagtttt
     6601 tatcacctaa ctcttgtttg gtggaatgat gccagcggtg cagctcggac gtgttgaaga
     6661 aaattgaaag catagggttt ctgaaatcaa tattcatatg attgaaaatg gagaagaagt
     6721 tattgaggct gcctgcaatc agtatggttt gtgagtctgc gccaagtagc agcagcgggc
     6781 caagacctgc cagaacgttc cacgcagcgt tgagtggatg ggtgcgcatt gcattcaatg
     6841 aataaactcg atccggcgca tgatgcaccg cgtggacttg ccacatgatc gcgctccatt
     6901 cgtgtgccgc ccggtgcagc agatagggac ccaatccaga gatcacaagg gcaaccagaa
     6961 cggagatcgg aagtggccat gatggccaga catgcgattc cataccggcg cgcgaaagga
     7021 ctacggtcgt tccgctcttg agaatcacag aaactaagat tgtgagacca ctgaatattg
     7081 cgtcgatctt tgaatcagtc ttgatccctt gccactctgc gtgatacggg atccatttct
     7141 caagagccat gccagccacc gcgaaggcca gcaagagaaa ggttgcactt tgcggaaaaa
     7201 accacagagt cattgtatat gtcatcagta aaaacgggaa gccgaagtct ttgatggtac
     7261 attcgatgga gcgtttgagc gatttgtctg ttttcattga gcggatacct tttgtattta
     7321 tatatttaaa tttatcaatg tattattgcg cagattttgc gttaagggat gcattagcag
     7381 catcctttca aaaagtgact gagtttatct accatagata tgatccgtgc ccggtccggt
     7441 cgatagtaga cttcttttcc gtgcttgcgg cgcaacagaa ggcctgcccg acagaga
//
LOCUS       DAORFF010000123         7482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4920_length_7482_cov_3.921907, whole genome shotgun sequence.
ACCESSION   DAORFF010000123 DAORFF010000000
VERSION     DAORFF010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4920_length_7482_cov_3.921907"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PKK76_06650"
     CDS             <1..800
                     /locus_tag="PKK76_06650"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNL68185.1"
                     /translation="VLNGKIADVRNGQTASINALVNFLLDWLTMHIQKEDKNYRDFLV
                     RSNANLREYFQKLVNAREITIDRGQVALYNMVEGKSEVGEILNESILSNIIKIWNTHN
                     LAVQIPIIDLQHIWLISMVVELDIASKTLGNTKRQKIFNKVIAGTVEYTREHFATEEK
                     IMERFAYKNTQNHIKQHRSFVEMVHRRQQENETGDARAAAHLVEDLKEWLVSHIAIED
                     RNIYLGLKDQLSEIQNYVRELINAGELVVKKKQVDMYNQVCGLKKAS"
     gene            882..1160
                     /locus_tag="PKK76_06655"
     CDS             882..1160
                     /locus_tag="PKK76_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68186.1"
                     /translation="MNLLELQKTVQEAIDKGARSVEQVHKQIANMPLEYLAKIAQLEG
                     PANQAKEFQTRTIGSIYDAIANINSKAGEIARDLLAKLPQGPGAPPKV"
     gene            complement(1164..1817)
                     /locus_tag="PKK76_06660"
     CDS             complement(1164..1817)
                     /locus_tag="PKK76_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNL68187.1"
                     /translation="MHETPFGTVPPSMLALVDEKKGTLNFKKGESIFHADDPAQAFYC
                     ISAGQVQLYRASRAKEQAFGVYGPGTWLGYRNALTGSAFDHSARCLTSVKVCKVDRAV
                     LEGFSIRFPSFTKAIVRELASGWTESEKQVYNLGARNVKERLAEYLLSLRKDTLAESH
                     DFDFPLTRETLASLMGTTTESVIRTLSDFRSRGWIDYHDGKISLSNERELEKLVVES"
     gene            complement(1923..2465)
                     /locus_tag="PKK76_06665"
     CDS             complement(1923..2465)
                     /locus_tag="PKK76_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68188.1"
                     /translation="MTEPKIARGTTLGFVPVIFYNLHAAYWIFRGGWENSLWACHVAS
                     LLIGAGMIFGTARLVLTGMLWLVIGNAFWILYLGTGGEFLPTSLLTHAGSFVIGLVGL
                     NRFGVVPGTWWRAALGLIPLHLAARFTPPAENVNLAHRVDQGWASVFPAHSTYVLFVF
                     ALCLVTFFVSERLFRKIKKG"
     gene            complement(2452..3168)
                     /locus_tag="PKK76_06670"
     CDS             complement(2452..3168)
                     /locus_tag="PKK76_06670"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HNL68189.1"
                     /translation="MIWLVVAFLILAGGELVAFVLGPSGFPDSIFRFLHLGQMVLLIV
                     ASFFVRAQLAARSAMELSEAAWNRAATLIQIGLVFSFVGDIINSGLIDLRSILKPQVL
                     ISIPFFATAHILYIRSYFIQTAFLSSRTIFLWVLWPFCAFALWKAVVDPSNTLIAALS
                     LPYSFMVSLMAISSIRMPMKYGLPGFTVTFGAFLFLLSDALIGWSITREHTRELSQVI
                     WSTYYTAQILILRSLYLDRT"
     gene            3266..3997
                     /locus_tag="PKK76_06675"
     CDS             3266..3997
                     /locus_tag="PKK76_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNL68190.1"
                     /translation="MNVIKSILFWLFLGITCISLFPIALVIRIVTGPFDRRLRLLHRF
                     TSFWASLYTWLNPFWGVSILHKTTVDPEQTYVIVSNHQSLVDIFAFFRTFVHFKWVSK
                     SENFKFPFVGWNMRLNRYIEIKRGSNRSSAEMMKQCENTLRSGSSIFIFPEGTRSTTG
                     MLGKFKMGAFELAKRTGLPILPMVVHGSSNALPKGGLILSGIHRIRIHIIDAITADEV
                     NRLSADELCDLTRNRIESELALMQG"
     gene            4001..4501
                     /locus_tag="PKK76_06680"
     CDS             4001..4501
                     /locus_tag="PKK76_06680"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNL68191.1"
                     /translation="MRKLFAYILGTVMSIMVVFFVVGLVAPKEYTGSKTAQISAPPSR
                     VWAVLTDIDSLPKNRPEIARVEVLEKDGPRAKKWIEHTDMDGFIVFERVEEIQNKKLV
                     LKMVESSFGMTGTWTYELSGNEMTQVTVKENSQIASVPLRAAMTLMGRDANLKKEIQI
                     INKAAQ"
     gene            complement(4531..5796)
                     /locus_tag="PKK76_06685"
     CDS             complement(4531..5796)
                     /locus_tag="PKK76_06685"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNL68192.1"
                     /translation="MSLQVYTNCDKYEGSFSGGSRHGPGEYTFANGDVYKGEFLNGNR
                     AGKGIYFFKSGQATDYSLMFDLDVNGTGTGILRANQKEIPCKILRGEALCGIGTKLRI
                     SEKMDPIAIVLYAGDSARIDRKGEPIAVSSGDPILPGDSIRSGSDNVDLQFKDNIAVR
                     AKPGSNLSFPREQERRIDLKEGAVTVKFGQDSGSLAVTAPAARVDADDSTFAVQTTAQ
                     KTDVKVFEINKGKVTVAPAIPELSGKSDAEIRKDPELSKLQEKQRSLSTVLNVNQSGN
                     LDRGTTATAEVKPFVPTVREKFESKVIVLADPEAVKKLSQDPGNAQAKIAMKQDYEKK
                     LDRAASSVETDLSEAPVSTEEDVMRRYGLVEYVQMLDKKMYKGCVVAQAGDTLILHGL
                     GAVRRIKVSDVNYIEYRHPGERAPEPSSP"
     gene            complement(5941..6879)
                     /locus_tag="PKK76_06690"
     CDS             complement(5941..6879)
                     /locus_tag="PKK76_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68193.1"
                     /translation="MRRFLTLVNIGMSSFARVFILLFASALHADTVLLVDGRLLEGKY
                     LGDRGDFIEWEQAGQIVKLESTNVVRIELAYPGVPVCFRRKGAKERVCQGKLYLLYGS
                     SVEIIGGPGDTKKETVKISDLSDLEFQRTTETQLLLPRIRPGAAIRLVIPEGSVQGSV
                     QSVEPGRVLVRDSQDQVVEVREEKIEQLQLPTSSGFLFSRLIPGLPQYRRSERIKSGA
                     IVGGIAFFGMSALWEQSQAQREVSRARANLLYISTGIGSRESVFAGHRKNAQILGFGM
                     LLTYGFHLTDEFVLSARSSRKSIQIGFSFPLALLLE"
     gene            6880..7254
                     /locus_tag="PKK76_06695"
     CDS             6880..7254
                     /locus_tag="PKK76_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68194.1"
                     /translation="MKQVIPRLGGSLRLEIFIIYAVLAVSTIVFFSTIILENQSDLLL
                     DLFRYQSRDVTQELGQALRKEKFDAAKPESLTQELAKRQITSYYVINAIGDCLFPAKC
                     TTPPGATDTIRTLISEAALFKN"
BASE COUNT         2081 a         1905 c         1780 g         1716 t
ORIGIN      
        1 aagtgctcaa tgggaaaatc gcagatgtaa gaaacggcca gacggcttct atcaatgcgc
       61 tcgtcaactt cctcctggac tggttgacca tgcacatcca gaaagaagac aagaactacc
      121 gcgactttct tgttagatca aacgcaaatc tacgggaata ctttcagaaa ctcgtcaacg
      181 cacgtgaaat cacaatcgac cggggtcagg tggccctcta caatatggta gaagggaaat
      241 cagaagtagg cgaaattcta aacgaaagca tactcagcaa catcatcaaa atctggaaca
      301 cgcacaacct ggcggtgcaa attccaatca ttgatctgca acacatatgg ttgatcagca
      361 tggtagtaga actcgacatc gcgagtaaga ctctgggaaa tacaaagagg cagaagatct
      421 tcaacaaagt gatcgcaggt acggtcgaat atactcggga acatttcgca acagaagaga
      481 aaatcatgga acgattcgct tataagaata cgcagaatca cataaagcaa catcgatcct
      541 tcgtagaaat ggttcataga agacaacagg agaatgaaac aggcgacgct cgcgcagcgg
      601 cgcacctggt ggaagacttg aaagaatggc ttgtgtctca tattgcgatt gaagacagaa
      661 atatttacct gggattgaaa gatcagcttt cggaaatcca gaactatgtc agggaactca
      721 tcaatgcagg agagctggtg gtgaaaaaga agcaagtgga catgtacaat caggtctgcg
      781 gtttgaagaa agcatcataa atcacttgcc ggcacaatcc agaccgacct aatacgacac
      841 cggccgggca accggtcgga atattgaatc ggggaaatca aatgaacctg ctagaactgc
      901 agaaaactgt tcaggaagcc atcgacaaag gtgctcgttc cgtagaacag gtacacaaac
      961 aaatcgcaaa catgcctctt gaatacctgg caaagattgc tcaactggaa ggaccggcta
     1021 atcaggcaaa agaattccag acaagaacca ttggaagcat ttacgacgcg attgcaaaca
     1081 tcaacagcaa agcaggcgag atcgctcgcg atttgcttgc gaagcttccc cagggcccag
     1141 gggctccacc aaaagtttaa cgattaagac tcaacgacaa gtttttcaag ctctctttca
     1201 ttcgaaaggg agatcttgcc gtcgtggtag tctatccacc cgcgcgagcg aaaatcagag
     1261 agagtgcgaa tcacagattc agttgtggtt cccatgagac ttgccagcgt ttcgcgcgtg
     1321 agtggaaaat caaaatcatg actctccgcg agagtatctt tgcgaaggga tagcaaatac
     1381 tcagccagcc tttctttgac gtttcttgca cctaaattat agacttgctt ttctgattca
     1441 gtccaacccg aagccagctc acgaacgatt gcttttgtaa aggatggaaa acggatcgag
     1501 aatccttcga gcacagcgcg atccaccttg cacactttca cagaagtaag acagcgagcg
     1561 ctgtgatcaa acgcactgcc tgtgagtgcg ttgcgataac ccagccatgt gcccggacca
     1621 tacactccga acgcctgttc tttcgcacga ctggctctgt acaattgcac ctgtcccgca
     1681 gaaatgcagt agaatgcctg ggctggatca tccgcgtgga aaatgctttc ccctttcttg
     1741 aaattcagcg ttcctttttt ctcatccaca agggcgagca tggaaggagg caccgtgccg
     1801 aaaggcgtct cgtgcatggt tgcgcattca gcgcacgatt cgctgcaaga atggaacatg
     1861 aggcccccag tgtgaacacg ccatccacgc cgtcaataaa tcatccaaaa ccatgatgaa
     1921 aatcatccct ttttgatttt gcgaaagagt ctctccgaga caaaaaacgt cacgagacat
     1981 aacgcgaaaa caaacaatac gtaggtgctg tgcgccggaa atacactggc ccagccctga
     2041 tccactctat gggccagatt cacgttttca gcaggcggcg tgaaacgggc ggcaaggtgg
     2101 agaggaatca atcccagagc ggccctccac caggttcccg gcaccactcc aaagcgattc
     2161 aacccaacca gtcctatcac aaaacttccg gcatgagtca gaagagaagt cggcaagaat
     2221 tctccgcccg tgcccagata caggatccag aaagcgtttc caatgaccag ccagagcatt
     2281 ccggtcagaa caagcctggc tgtgccgaaa atcattccgg ccccgatcaa aagggaagca
     2341 acgtggcatg cccagagcga attctcccag ccaccgcgaa agatccaata ggcagcatgc
     2401 aaattgtaga agatgacggg aacaaagccc agcgtagtcc cgcgggcgat tttaggttct
     2461 gtcaagatag agagacctca aaatcaatat ctgtgcggtg tagtatgtgg accagattac
     2521 ctgactcaat tcgcgggtat gttcgcgcgt gatgctccag cctatgagag cgtccgaaag
     2581 caaaaacaga aaggcaccaa atgtcacagt gaaaccaggc aaaccatact tcatgggcat
     2641 gcgaatcgaa gaaatcgcca taagcgaaac cataaaagaa tacggaagag aaagtgcggc
     2701 aatcaaggtg ttcgatggat ccactacagc tttccacagc gcgaaagcac agaaaggcca
     2761 gagaacccac agaaatatgg tgcgcgaaga gagaaatgct gtttgaatga aataactgcg
     2821 aatatacagg atatgtgcag ttgcaaaaaa tggaatggaa attaaaacct gcggtttcag
     2881 aatgctgcgc aagtcgatta gaccgctatt gatgatgtct cccacaaatg aaaagacgag
     2941 tccaatctgt atgagagtgg cggcccgatt ccaggcggcc tctgaaagct ccatagcaga
     3001 gcgggcggcc agttgagcgc gaacaaaaaa ggacgcgacg atcaggagaa ccatctgtcc
     3061 aagatgcaag aagcgaaata tagaatcagg aaagccggaa ggcccgagca cgaaagctac
     3121 aagctctccg cctgcgagaa tgaggaaagc aactacgagc cagatcatgt gctgattcga
     3181 cggatcaagg cccttgcgtc aagtatcctt gctctttttt gcaactggtt gccccacttt
     3241 gagtcgaatt tagaggtgaa gattcatgaa cgtaattaag tctatactat tctggttgtt
     3301 tcttggtatc acctgcatca gccttttccc gatcgcactc gtgatccgga tcgtaacggg
     3361 gccgtttgac cgtcgtcttc gcctgctaca tcgcttcacc tctttctggg cttcgctcta
     3421 cacctggttg aatcctttct ggggtgtaag catccttcac aaaaccactg tagatcctga
     3481 acagacttac gtgatcgtat ccaatcatca gtcacttgtg gacatcttcg cattctttcg
     3541 cacattcgtt catttcaaat gggtcagcaa atcagagaat ttcaaattcc catttgttgg
     3601 ctggaacatg aggctcaatc gatacataga aatcaaacgg ggcagcaatc gcagcagcgc
     3661 agaaatgatg aagcaatgcg aaaacactct gcgcagtgga agttccatct tcattttccc
     3721 ggaaggaaca agatccacga caggaatgct gggtaagttc aagatgggcg cgtttgaact
     3781 tgcgaaaaga acaggtcttc cgattcttcc tatggtggtg cacggttcgt ccaatgctct
     3841 tccgaaaggc ggtctcatcc tgagcggaat tcatcgcatt cgcattcaca tcatagatgc
     3901 aatcacggca gatgaagtaa atcgcctgag tgcggatgag ttgtgcgatc tcacgcgaaa
     3961 tcgtatcgaa agtgaactcg ctttgatgca aggatagtgt atgcgtaaac tgtttgccta
     4021 catcctcgga accgtgatga gcatcatggt tgtattcttt gtagttgggt tggttgcccc
     4081 aaaagaatac actggatcca aaaccgcgca gatttctgcg ccaccttccc gcgtctgggc
     4141 cgtcctgacg gatattgata gcctgccaaa gaatcggcct gaaattgcgc gagtagaagt
     4201 gctcgaaaaa gatggcccgc gggcaaagaa gtggatcgaa cacacggaca tggacggctt
     4261 cattgttttt gaaagagtgg aggagattca aaacaaaaaa ctggttttga aaatggtaga
     4321 gagctcattc ggaatgacag gaacctggac gtatgaactt tccggcaatg aaatgactca
     4381 ggttaccgtg aaagaaaata gccagatcgc aagcgttcct ctccgcgctg ccatgactct
     4441 gatggggcgg gacgcaaatc ttaagaaaga aatacagatc ataaacaaag ccgcgcaatg
     4501 aaatggcggc cggacattca acaatgattc ctacggactg gaaggttcag gcgccctttc
     4561 tccgggatgc ctgtattcaa tgtagttcac gtccgatacc ttaatgcgtc tcactgcacc
     4621 caatccgtgc agaatcaggg tgtctcctgc ctgcgcaaca acacatccct tatacatctt
     4681 cttatctagc atctgcacat attccacaag gccgtatcgc cgcataacgt cttcctccgt
     4741 agaaaccgga gcctctgaaa gatccgtttc cacactcgaa gctgcgcgat ccaatttttt
     4801 ttcataatcc tgcttcattg caatcttcgc ctgcgcattt cctggatcct gagagagttt
     4861 cttcaccgcc tcaggatctg cgaggacaat cactttactt tcaaattttt cgcgaacagt
     4921 ggggacaaaa ggtttcacct ctgctgtcgc ggtagtgcca cgatccagat ttccagattg
     4981 attcacattc aagactgtgc tgagacttct ctgcttttcc tgaagttttg atagttcagg
     5041 atctttacga atttccgcat cgctttttcc actgagttct ggaatcgcgg gcgctacagt
     5101 gaccttacct ttgttgatct caaagacttt tacatctgtc ttttgcgccg ttgtctgaac
     5161 cgcgaaagta gaatcatccg catctactct ggcggccggg gcagttactg caagactccc
     5221 tgaatcctgt ccgaatttga cagtaactgc tccctctttt agatcaattc gcctttcctg
     5281 ttctctggga aaggatagat tggatccagg cttcgcgcga actgcaatgt tgtctttaaa
     5341 ttgcaaatcc acattgtcgg aaccggatcg aatggaatct cccggcagaa tgggatcccc
     5401 ggaggatacg gcgataggtt cgcccttgcg atcaatgcgc gcagaatctc cagcatataa
     5461 aacaattgcg attggatcca tcttctccga aattctgagt ttggtaccaa tgccgcataa
     5521 cgcttccccc ctcaaaatct tgcatggaat ttctttttga ttcgcgcgga gaattccagt
     5581 cccggttccg ttcacatcca ggtcaaacat cagggaatag tcggtggctt gtccgctttt
     5641 gaaaaagtag atacccttcc cggcgcgatt cccgttcagg aactcccctt tgtacacgtc
     5701 tccattggca aaagtgtatt ctccgggtcc atgacgacta ccgccagaaa aagaaccctc
     5761 gtatttatca caatttgtgt aaacctgcaa gctcacggta ttcttacaat cgccactgat
     5821 gcatgcattg ttgcaaattt ctcgagcgga aagagcagtg ccaaaaagaa tcaatgatat
     5881 tccagtcagc cagagcaatc gcatgggttc atagaggacc caacaacgga atgggtcaag
     5941 tcattccaga agcaatgcca gcgggaagga aaaaccgatc tgaatcgact ttcgagacga
     6001 tcgagcgctc agtacaaatt catctgtcag gtgaaatcca tatgtaagaa gcattccaaa
     6061 tcctagaatc tgcgcgttct ttctgtgccc ggcaaaaaca gattcacgcg acccaattcc
     6121 ggtactaatg tacaacaaat tcgccctggc ccgagaaact tctcgctgag cctggctttg
     6181 ttcccagagc gcagacatac caaagaaagc aattccacca acaatcgccc ccgacttgat
     6241 gcgttctgat cttctgtatt ggggcagtcc cgggatgagc cttgaaaaca aaaaaccact
     6301 ggaagtggga agctgcagtt gctcgatttt ttcttcgcgc acttccacaa cttgatcctg
     6361 cgaatcgcgg acgagaacgc gtcctggctc caccgactgc acactaccct gaacgctgcc
     6421 ctccggaatc accaggcgaa tcgcagcccc tggccgaatt cgcggaagaa gtaactgggt
     6481 ttccgtcgtg cgctgaaact ctagatcaga gagatcagaa atcttaaccg tctccttttt
     6541 tgtgtcgccc gggccgccga tgatttccac agagcttccg taaagaaggt aaagctttcc
     6601 ctggcagacg cgctccttcg cgccctttcg gcgaaagcaa accggtactc ccggataggc
     6661 gagctcaatt cgcacgacgt tggtagattc gagctttacg atttgccctg cttgctccca
     6721 ctcgatgaaa tcgccgcgat cacccagata tttaccttcc agtaatcgtc cgtcgacgag
     6781 aaggacagtg tcagcgtgca atgcggacgc gaacagtagg atgaaaaccc gtgcgaaaga
     6841 ggacattccg atgttgacca gggtgaggaa gcgtcgcaca tgaagcaagt cattccgcgc
     6901 ctgggcgggt ctcttcgcct tgagatattc ataatctacg cggtcctcgc cgtctcaacc
     6961 atcgtgttct tctctaccat catcctggaa aaccagtctg atctgctgct ggatttattc
     7021 cgctaccagt cacgagatgt gacgcaggaa ctgggccagg ctcttcgcaa ggagaagttc
     7081 gacgcggcga aaccggaatc gctaacgcag gagcttgcga aacgccaaat aacgtcttac
     7141 tacgtcataa atgcaattgg cgattgccta tttccggcaa agtgcaccac gcccccgggc
     7201 gccactgata cgattcgcac gctcatttcg gaggcagccc tcttcaaaaa ctgacagtca
     7261 tatatccctg gatccagact cgggcacagc cagctttgtg ctcagcctgg atgattcgca
     7321 acggctcttc ttgcaaggtt ccttgaaggt atctgcgatg gaagcgcgta tgtcccaggt
     7381 gaaccgacag ctctggctgg tcctgggctg gatggttgta tttaacgtcg catttggaat
     7441 ctatgtgtat cgcagctgcg atacacatag attccaaatg cg
//
LOCUS       DAORFF010000124         7482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4921_length_7482_cov_3.840851, whole genome shotgun sequence.
ACCESSION   DAORFF010000124 DAORFF010000000
VERSION     DAORFF010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4921_length_7482_cov_3.840851"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PKK76_06700"
     CDS             <1..652
                     /locus_tag="PKK76_06700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNL68195.1"
                     /translation="YPRVRVEKRRFDDFVRQKNHVISLAKCPWILVVDADEVVTPALR
                     DEISQVIGNNISAEGYRVPRMTFYLGKWIKHCGWFPDYNIRLFRRGRARFEGGTVHEK
                     VAVDGTIGTLKGHLQHYSYRTTSDHLIRMDQYSTLLAEDKFLKGKRSGPLLAGYKSLS
                     KFLLIYFWKLGFLDGRPGFVIAVMGAYYNFLKYIKLWELRRGLRKPGDRVRYPGNV"
     gene            652..1854
                     /locus_tag="PKK76_06705"
     CDS             652..1854
                     /locus_tag="PKK76_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNL68196.1"
                     /translation="MSIKQKLGLYKPSLQPIPEVRIQRSFGTVKCNELGPYVLERTIS
                     TDSLKRGDRLLSDYHPFRVSGILELALVRENHPVHPEDLLFLDTETTGLSRGAGNFPF
                     LVGLGYFENGSLVIRQILMTSPAGEKQFLDAIQEEVRKHSHLVTYNGKSFDIPLIKNR
                     LILQRDRLHPPVLHFDLFHILKRLFPHRRLGRYRQKDLEAELLGFSREDDIEGAQVPQ
                     IYFDFVKYGTDTGMEKVIEHNRLDLEGMMLLFLEAVRVYTDLDSSRASLRSGLARILA
                     HNHRYFEALDAAMEIQDLLLDGKEGFHYRDALFRAWLERRAGNYEEAASTYETIAERC
                     MCDYSRLNLVRILEYRLNRPHQAIEHVDVLISRAPPPDLPSLARRRARLDMKAKKLDE
                     RRGRESSA"
     gene            1886..2278
                     /locus_tag="PKK76_06710"
     CDS             1886..2278
                     /locus_tag="PKK76_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68197.1"
                     /translation="MKRIFLLLLTFSAWGLQIQAEQTTAPSSSGKKPFVRPVLVPSFG
                     EPASGRLGWEQAVSYCLEQKSRLPTVEELKLAFQNGLLKTWEQQGDYWTLSDDADQSI
                     AYSFITKFGLAHAESKGHGNLVRCVKPN"
     gene            complement(2271..3485)
                     /locus_tag="PKK76_06715"
     CDS             complement(2271..3485)
                     /locus_tag="PKK76_06715"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNL68198.1"
                     /translation="MKETRIQTLGLSKIFRLYKSRLDRIRELLHPTRKKFHNDFYALK
                     EIDLTVSAGESIGIIGRNGSGKSTLLKIIAGVMTQSAGTVSISGRIASLLELGAGFHP
                     ELNAVENLYLHGLLRGIPRSQTKKKLDSILDFSGIGPYAHQPVKSYSSGMFVRLAFSA
                     EVHTIPEIFIVDEALGVGDASFQMKSMRRMRELQEQGTTIAFVSHDASTVRSLCTRAI
                     LLDKGRIVAEGSPAQVYDIYSGMIGFADEAVEANASFAERSGSGELRFAEAWIENSSG
                     KRADTFRSGEGARVVLEIEASETIENPTIGIHFRDASSHELFGINTALLGIDTGIFVS
                     GQKRRVAFTMPLDFGPGLCTLGTSIHQFEIHTGSCYDWVNHAITLRIEPDPSYRFSGS
                     ARLRASFLSSVS"
     gene            complement(3482..4348)
                     /locus_tag="PKK76_06720"
     CDS             complement(3482..4348)
                     /locus_tag="PKK76_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNL68199.1"
                     /translation="MKGHNRLLREIRQENSASYHLTRMVASPGWSVLSVRKLWQNRIV
                     LFQLAVYDFRHEYLGTWFGFLWAFVHPLLSASILWFVFSHGFRASRTAGDHFFLWLLS
                     GLFAWQFASLSIRGGALSIVQNSFLIRKLAFHVEFLPIVKIISASFLHAAFIVGLLIL
                     SWKDRGPDVANFWIAYYWLCALVLSLGIAWLASAATVFVRDFAGVVEIGLQIAFLGTP
                     IFWDLSILPESIQWIAALNPLYYIVTGYRHALLGLGPPSGHLEFWIPALLVFFVGQFV
                     FKKLRPIFADFI"
     gene            4347..6611
                     /locus_tag="PKK76_06725"
     CDS             4347..6611
                     /locus_tag="PKK76_06725"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNL68200.1"
                     /translation="MNLPQFLDFLKQDIQFGKNLTGAFTIAGQDASFAPIPSSLDSRL
                     VAGLGARGLDKLYTHQALAVSSALAGKNTLVVTPTASGKSLTYMLPIFQKKLSSPQSR
                     ALLLFPTKALSQDQQASINEFNTHCDTNFKIFTFDGDTSPEARRRIMEAGDFVVTNPD
                     MLHAGILPHHTLWIKLFENLDFIVIDELHIYRGIFGSHVANLLRRLLRICAFYGKHPQ
                     ILCASATIGNPKEHAERLTGLPFELIDQNGAPRGDRHVLFYNPPVVNQQLGIRASALK
                     ETAALGSYLLKNRIRVIFFCRSRLRVELLYTYLVERCPELKDKIRSYRGGYLPDERRR
                     IERELREGSVLAVVSTNALELGVDIGMLDVAVCMGYPGSVSSLLQQFGRAGRRNQESL
                     SILVATSDGTDQYLIQHPDQFFEKNPEQAMTNPDNLLIVADHIKCATFELPFKKEESF
                     GSYPATLEILEHLAENRVIHGSGESFHWMSDVYPANTISLRSGPRENFAIIDCTEKGR
                     EEVIGEVDYFSAPTMIHQHAIYIHQGVQYYVDDLLWEERQARVRRIEVDYYTDAHEKV
                     DIEVLEDQARRVHNWFELSRGEFSIRTKAVMFKKIKLETHENLGWGDIHTPETEMHTQ
                     GAWILIPEDHPVRAGLAESELGAMLAGVSYSIGVVSPLFVLCDPRDMRFRSEVRSTVF
                     HKPAIFFYDSFPGGMELSHRVLENLAGIALSAHELVSRCGCANGCPSCIGLPDDESPV
                     KANVLKFLTALCDA"
     gene            complement(6615..7160)
                     /locus_tag="PKK76_06730"
     CDS             complement(6615..7160)
                     /locus_tag="PKK76_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68201.1"
                     /translation="MSDAWDDLSDHELESLRKAEEKFKVIKQQSNPFEHLQALAQREI
                     FKVNFSSYSGKDAGDASPLVIHADRTRLLPLISVAHTMWLPEFEEVATLVLDAEAAAK
                     KGEPESDDLEWNLQSFGLRRTPESYIVGYGENSSPLVLNKKMLLEFLSFLASSLSILE
                     HREGRNGQTMADLEPRFLQFI"
     gene            complement(7162..>7482)
                     /locus_tag="PKK76_06735"
     CDS             complement(7162..>7482)
                     /locus_tag="PKK76_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68202.1"
                     /translation="KPLKILKQAGAQGAKPADRITIVSLDESNIFTFSASVTTWKKEA
                     LDYVVESVHIEVGPYSKMSLPWRERSYVVFGLRQFGYPGTVLLDILTSPVQLLYLAAG
                     PGAK"
BASE COUNT         1876 a         1831 c         1862 g         1913 t
ORIGIN      
        1 ctacccacga gtccgagtgg aaaagcgcag attcgacgac tttgtgcgtc agaaaaatca
       61 tgtcatttcg ctggccaagt gtccgtggat tctggtggtg gacgccgatg aagtagtcac
      121 tcctgcactc cgtgacgaaa tttcgcaggt gattggaaat aatatatccg cagaagggta
      181 tcgcgtgccc agaatgactt tctatctggg aaaatggatc aaacattgcg gatggtttcc
      241 tgattacaac atcagactct ttcgccgcgg gcgagcgcga tttgaaggcg gcacggttca
      301 cgaaaaagtc gcagtggatg gaacgattgg tactctgaaa ggccatctgc agcattattc
      361 ttacagaacc acaagtgatc atctcattcg aatggatcaa tactccactc tgcttgcgga
      421 agataaattt ctgaaaggaa agcgatcggg gccactcctt gcgggctaca agagtttgag
      481 taagttcctt ctcatctatt tctggaagct ggggtttttg gacggtcgcc ctggttttgt
      541 gatcgccgtg atgggagcat actataactt tctaaagtat atcaaactct gggagttgcg
      601 ccggggattg cgtaaacccg gagatagagt tcggtacccc ggaaatgtct aatgtccatc
      661 aagcaaaaac ttggtctgta caaaccatct ctgcaaccga ttccagaagt tcgcattcag
      721 cgaagttttg ggacggttaa gtgtaacgaa ctcggaccgt acgtcctcga aagaactatt
      781 tccacggatt ctctaaagcg cggagatcgc cttctttctg actatcatcc ctttcgcgta
      841 agtggcattc tggaacttgc tctcgtgcgc gaaaatcatc ccgtacatcc ggaagacttg
      901 ctttttctgg atacagagac aacgggtctg tcgcgagggg cgggaaattt tccgtttctg
      961 gttggccttg gatatttcga aaacggatcc cttgtgattc gccagattct catgacctct
     1021 cccgcaggag aaaagcaatt cctggacgca atccaggaag aagttcgcaa gcactctcat
     1081 cttgtaactt acaacggaaa gtcttttgat attcctctga tcaagaatcg tctgatcctg
     1141 cagagagatc gactgcatcc gcctgtttta catttcgatt tgtttcatat tctaaagaga
     1201 ctctttcctc atcgcagact gggacgatac agacagaagg atctggaggc tgagcttctg
     1261 ggtttttcac gcgaagatga cattgagggc gctcaagtgc ctcagatata tttcgatttc
     1321 gtgaaatatg gtacggacac tggaatggaa aaagtcatcg aacacaatcg actcgatctg
     1381 gaaggaatga tgcttctttt tctagaagca gtgcgagtgt atacggatct tgattcttcg
     1441 cgtgcatctc ttcgttctgg ccttgcgcga attctcgccc acaatcatcg ctattttgaa
     1501 gctctggatg cggcaatgga aattcaggat ctgttgctgg acggaaaaga aggatttcat
     1561 tatcgcgatg cactgtttcg cgcatggctg gagcgccgtg caggcaatta cgaagaggca
     1621 gcgtccacct acgaaaccat agcagagcgc tgcatgtgcg actattccag attgaacctg
     1681 gtaaggattc tggaatacag attgaatcgc cctcatcagg cgatcgagca tgtagacgtt
     1741 ttgatttcca gagcgccgcc cccagatctg ccatcccttg cccgccgtcg cgcccgcctg
     1801 gatatgaaag caaagaaact ggatgaacgt cgcggccgtg agtcttccgc ttgaccagga
     1861 gcaaggggga ggaaagtcta aggtcatgaa acgcatcttc ctgcttttgt tgactttctc
     1921 agcatggggc ctgcaaattc aggcagagca aaccactgca ccttcttcat ccggtaagaa
     1981 accatttgtt cgaccggtgc tggttccatc tttcggtgaa cctgccagcg gaagattggg
     2041 atgggagcag gcagtgtcct attgcctgga gcaaaagtct cgtcttccta ctgtggaaga
     2101 attgaaactg gcgtttcaga acgggttatt gaaaacctgg gagcaacagg gagactactg
     2161 gacgttatcc gacgatgcag atcaatcgat cgcctattct ttcatcacaa agtttggact
     2221 ggcgcacgca gaatccaaag gccacggaaa cctggttcgt tgcgtcaaac ctaactgaca
     2281 ctgctcagga agcttgcgcg caatcttgcg cttcctgaaa aacgataaga cggatccggt
     2341 tcgattcgaa gtgtaattgc atgattcacc cagtcgtaac aggagccggt gtggatttca
     2401 aattgatgaa tggatgtgcc aagagtgcac aggccgggtc caaaatccag aggcatggtg
     2461 aatgcgacgc gccttttctg tcccgataca aaaatcccag tatctatccc aagcaatgca
     2521 gtgttgattc caaagagttc atgactcgat gcgtcgcgga agtgaattcc aatagttgga
     2581 ttttctatag tttcggacgc ttcaatttcg agtaccactc gcgcgccttc ccctgagcga
     2641 aatgtgtccg ctctctttcc ggatgagttt tctatccatg cttccgcgaa tcgcaattct
     2701 ccggatccac ttctctctgc gaatgacgca ttggcctcta ctgcttcgtc tgcaaatcca
     2761 atcatgccgc tgtaaatgtc gtacacctgc gcggggcttc cctctgccac gattctacct
     2821 ttatcgagca ggatagctct cgtgcagagg cttcggacag tcgatgcgtc atgacttaca
     2881 aaagctatgg tggttccctg ctcctggagc tctcgcattc tcctcataga tttcatttga
     2941 aacgaggcgt cgcctacacc aagtgcttcg tctacaatga agatttcagg aatggtgtgg
     3001 acctccgcgg aaaaggcgag ccgcacaaac attccactcg aataagactt cactggctga
     3061 tgcgcatacg ggccgattcc agaaaaatcc aggatcgaat cgagtttctt tttggtttga
     3121 gatcgtggaa ttcctctgag cagcccgtgc aggtatagat tctctactgc gttgagctca
     3181 gggtgaaatc ctgcaccgag ttccagaaga gatgcaatcc ttccagaaat agataccgtg
     3241 cccgcggact gtgtcatgac tccggcgatg atcttcagca gagtggattt accgcttccg
     3301 tttctgccaa tgattccaat gctttcgccc gctgaaacgg tcagatcgat ttccttcaat
     3361 gcgtagaagt cattgtggaa ctttttgcgc gtcgggtgaa gaagttcccg gatgcgatcc
     3421 aatctacttt tatacagtct gaaaatcttt gaaagaccaa gcgtttggat gcgagtttct
     3481 ttcatatgaa gtccgcgaag atgggcctga gttttttgaa gacaaattgt ccaacgaaga
     3541 ataccagaag ggccggaatc cagaattcca gatgaccgga cggcgggccc aggcccagga
     3601 gcgcatgtct gtagcctgtt actatgtagt acaaaggatt cagagccgca atccattgaa
     3661 tcgattcggg cagaatactc aaatcccaga aaatgggggt cccaagaaat gcaatctgca
     3721 ggccgatttc taccacgcct gcgaaatctc gaacgaacac agtggccgca gaggcaagcc
     3781 atgctattcc aagggagaga accagtgcgc ataaccagta gtatgcgatc cagaaatttg
     3841 caacatctgg tcccctgtct ttccaggaca ggatcagcag cccgacaatg aatgctgcgt
     3901 gcaaaaagga agcggatatg attttcacga tcggcagaaa ctctacatgg aacgcgagct
     3961 ttcggatcaa gaagctgttt tgaacaatgg aaagggcccc accccgaatt gacagggatg
     4021 caaactgcca ggcaaacaat ccggacagta accacagaaa gaaatgatca cccgccgtcc
     4081 gggatgcgcg aaatccgtga gagaatacga accacagaat gcttgcgctt aacaacgggt
     4141 gaacaaaggc ccagagaaaa ccaaaccatg tgcccagata ttcatgtctg aagtcataga
     4201 ccgcaagctg aaacagtacg attctattct gccatagttt gcgcacggag agcacgctcc
     4261 agcctgggct tgcaaccatg cgagtcaagt gatacgaagc ggaattctct tgccggatct
     4321 cgcgaaggag gcgattatgt cccttcatga atctccctca gttcctggat tttctgaaac
     4381 aagacattca gttcggaaag aacctgaccg gagcattcac gatcgcaggt caggatgctt
     4441 cctttgcgcc cattccatcg tccctggatt caagacttgt ggccgggctt ggtgcgcgcg
     4501 gcctcgacaa actttacact caccaggcgc ttgcggtttc ttctgctctt gcaggcaaga
     4561 atactctggt cgtgactccc accgcatccg gcaaatcgct cacttacatg cttccgattt
     4621 ttcagaagaa gctctcatcc cctcaaagcc gcgcattgct gttgtttcca acaaaggctc
     4681 tcagtcagga tcagcaagcg tccatcaatg agttcaatac ccattgcgat acaaacttta
     4741 aaatatttac ttttgacgga gatacttctc cggaagcgcg aagacgaatc atggaggctg
     4801 gtgatttcgt tgtaaccaat cctgacatgt tgcacgcggg cattctaccg catcacacac
     4861 tgtggatcaa actattcgag aatctggatt tcattgtaat tgatgagctc cacatctacc
     4921 gcggaatttt tggaagtcat gtggccaacc ttcttcgccg attgcttcgc atctgcgcgt
     4981 tttacgggaa gcatccacag attctttgcg cgtctgccac cattggaaat ccaaaagaac
     5041 atgctgagcg cctaacgggt ctaccatttg aattgatcga tcagaacgga gctcctcgtg
     5101 gagatcgtca tgtattgttc tacaatcccc ctgttgtaaa tcagcagctg ggaatcagag
     5161 cgtctgctct caaggaaaca gcagcccttg gatcctacct gctcaagaat cgaattcggg
     5221 tgatcttctt ttgcagaagc aggctcagag tggagctcct ctatacttat cttgtagaaa
     5281 ggtgtcctga actaaaagac aagattcgat cctacagagg tggctatctt ccggatgaac
     5341 gccgtcgtat cgaaagagaa ttgcgcgaag gatccgttct cgccgtggtc agcacgaatg
     5401 ctctcgagct gggtgtggat atcggcatgc tggacgttgc cgtttgcatg ggatatcctg
     5461 gttccgtcag ttcattgctt cagcagtttg gccgcgccgg cagaaggaat caagagtctc
     5521 tttctattct ggtggccact tcagatggaa cggatcagta cttgatccag catccagatc
     5581 agtttttcga gaagaatcct gagcaggcaa tgaccaaccc tgacaatctg ctgattgttg
     5641 cagatcacat caaatgcgca acgttcgagc ttcctttcaa gaaggaagaa tcatttggat
     5701 cgtatcctgc aactctagaa atcctggaac acctggcaga aaatcgtgtg attcatggat
     5761 ccggtgagtc gtttcactgg atgagcgacg tatatcccgc taatacgatc agcctcaggt
     5821 ctggcccgcg cgagaatttt gcgatcatag attgtacgga aaaaggacga gaagaagtca
     5881 taggcgaagt ggattatttt tcagcaccta ctatgattca tcaacacgcg atttacattc
     5941 accagggcgt tcagtactac gtagatgatt tattgtggga agaacgtcag gcgcgcgtgc
     6001 gcagaatcga agtggattac tacacggacg cgcacgaaaa ggtggatata gaagtgctcg
     6061 aggaccaggc caggcgagtc cacaactggt ttgaactttc tcgaggcgaa ttttccattc
     6121 gcacaaaggc ggtgatgttt aagaagatca agcttgagac tcatgaaaat cttggctggg
     6181 gagatatcca cacaccagaa actgaaatgc acactcaggg tgcctggatt ctgattcccg
     6241 aggatcatcc agtgagagcc gggctggcgg agagcgagct gggtgccatg cttgcgggag
     6301 tttcctattc tattggagtg gtctcgcctt tgtttgtgct ttgcgatcca cgcgatatgc
     6361 gatttcgttc tgaggttcgt tctactgtct ttcacaagcc tgcgattttc ttttacgatt
     6421 catttccagg tggaatggag ttgagtcatc gtgtgctcga aaacctggca ggcattgcac
     6481 tgtctgcgca cgagctcgtg agtagatgcg gatgcgcgaa cggctgtcca tcgtgcattg
     6541 gtttgccgga cgacgaatct cctgtgaagg ccaatgtgct caagtttctc acggcgcttt
     6601 gtgatgcgtg agttctaaat aaattgaaga aagcgcggtt ctaaatccgc catggtctgg
     6661 ccgtttcttc cttctctatg ttcaagaatg gaaagtgaac tggccagaaa ggaaagaaac
     6721 tcgagaagca tcttcttgtt cagtacaagc ggagaactat tttctccgta gcctacgatg
     6781 tacgattcgg gagttcttcg caatccaaag ctttgcagat tccattccag atcgtctgat
     6841 tcaggttctc ctttcttcgc cgcggcctct gcatccagaa cgagtgtagc cacttcttcg
     6901 aattcaggca gccacatagt gtgtgctact gaaatcaagg gcagcaatcg agtcctatcc
     6961 gcatgaatga ccagcggact ggcgtctcct gcgtctttgc cgctgtagga cgaaaagttc
     7021 actttgaaga tttctctttg tgccagtgcc tgcaaatgct caaacggatt tgattgttgt
     7081 ttgatgactt tgaacttctc ttctgccttg cgcagggatt ccagttcatg atcgctcaaa
     7141 tcatcccacg catcgctcat gtcatttggc tcccgggcct gccgccagat acagtagttg
     7201 cacagggctc gtgaggatgt ccagaagaac ggtcccagga taaccaaatt gcctcagtcc
     7261 aaaaacaacg taactgcgct ctctccacgg aagagacatt ttggaatatg gacccacttc
     7321 gatgtgcact gactctacta cataatccag ggcttctttt ttccatgtgg tgacggatgc
     7381 ggaaaaggtg aagatattgg attcgtctaa actaacaatg gtaatgcgat cggccggctt
     7441 tgcgccttgt gctcccgcct gcttcaatat tttcaggggc tt
//
LOCUS       DAORFF010000125         7455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4948_length_7455_cov_4.592432, whole genome shotgun sequence.
ACCESSION   DAORFF010000125 DAORFF010000000
VERSION     DAORFF010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4948_length_7455_cov_4.592432"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PKK76_06740"
     CDS             complement(<1..660)
                     /locus_tag="PKK76_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68203.1"
                     /translation="MRYETLRGPSISRLMSEMRQKHGSAAVLIKQREVKEGGLLGTGL
                     LSRKIYEIHYMVPEKSVEQTASKKVRPAEFTKISRQELARKLKPVFEDESLPETVAQA
                     TPAIPTPQAISEAKPEIKLERESTPQYFLKIRAQLLGAKLSATYADSIIAHLESNLSK
                     NDRAQPQKVEQKALERLAETIRTVPDIAPPRGEARAVMLIGPTGMGKTTSLAKLAARY
                     FF"
     gene            691..1371
                     /locus_tag="PKK76_06745"
     CDS             691..1371
                     /locus_tag="PKK76_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68204.1"
                     /translation="MLAIVFVVLLDFCAFFQSFLIVYRRVETKGCDVRFTLCLAVLFF
                     AVSCGKHRPDPDKPPLHLFVWGKNRGDVKSLLEKEGYLVSERTEFIRATVPESEGRDI
                     MPDESPYQLTFYFQNDRLNIVQIQIRNSPDRTTIYEQNLKTAYKLAQPVLQKSLPPRT
                     TEAGNQIKETHSLYDTGEVYIKLYKSQVEIAESRMEEVPDETDIMIYSRKENEGITAE
                     GLLSPEEN"
     gene            1430..2485
                     /locus_tag="PKK76_06750"
     CDS             1430..2485
                     /locus_tag="PKK76_06750"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNL68205.1"
                     /translation="MDLAKVRELLGGQAESLLGHKAKVAKETIHLPGPDFVDRIWTQS
                     DRSPQVLRSLQQIFSHGRLGGSGYVSILPVDQGVEHSGGASFAPNPIYFDPENIVKLA
                     IEGGCNAVCSTFGVLGSVARKYAHRIPFLVKINHNEFLSYPNKYDQVMFGTIKEAWNM
                     GAVAIGATIYFGSDESRRQIVEVSEAFAYAHELGMATVLWCYLRNNAFKVDGVDYHTS
                     ADLSSQGNHLGVTIQADIIKQKLPTNNGGYNATKHGKTHPKVYSDLSTDHPIDLTRYQ
                     VLNCYSGRAGLINSGGESKGSSDLAEAVGTAVINKRAGGMGLISGRKAFQRPFEEGAQ
                     LLQSIQDIYLNKDITVA"
     gene            2486..3115
                     /locus_tag="PKK76_06755"
     CDS             2486..3115
                     /locus_tag="PKK76_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNL68206.1"
                     /translation="MDLLNSLLGVVQNFSGPLPYLFVFFILLLCGFGLPLPEDVVLFT
                     AGVLAYKGLADIHIMVAVAFAGVMIGDGTIFFLGHKLGLRLTQLPFFQRVLSPVRLAA
                     IRERFHEHGNKIVFAARFMPGLRTPVFFTAGTLHLPFRVFLFYDGMAALISVPTITYV
                     VFLFGHELEAVINTVRSVEHGMVLLIALVIAFFVGKFLWGRRKSANAPE"
     gene            3169..3969
                     /locus_tag="PKK76_06760"
     CDS             3169..3969
                     /locus_tag="PKK76_06760"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNL68207.1"
                     /translation="MLFFQPVGKHRFSTPLAVYALIAISVLAYAFTALDSENIIKSFG
                     LVPARFSFIDSVISTFLHSGIFHLIGNMIFLYMFGPGVEDVVGPFMFVLLYLILAVIS
                     DSCYAWMNLQSTLPVVGASGAVSGILGVFLVFFPNVKVNLFLAADSEASASGPFLMNA
                     FWAIGFWFAGQLLWAILALALPTHVAYAAHVGGLIGGVILGLLLKAAGFLDSFHARVR
                     RTRGSSKPTVYCPACALPIAPTEFGRYRCPNCASEFTFDVKRGYRLLQ"
     gene            complement(3999..4568)
                     /locus_tag="PKK76_06765"
     CDS             complement(3999..4568)
                     /locus_tag="PKK76_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNL68208.1"
                     /translation="MRDVLQESVLVLNTSMIAIDICSARTAILEILRNIAIPIKESED
                     LVRSEKLTLHVPRIIAQRSYHKIPRYDQKTSKINIIYRDDQTCCYCGVRFPIPELTVD
                     HVIPRSRWKKITGETPPYDFNSWWNLVTACRRCNSVKANRLLQEIGWKLQKKPDKPKN
                     TGLVIARQRAESLGWMEYCRVNVRLVESV"
     gene            complement(4612..7092)
                     /gene="gyrA"
                     /locus_tag="PKK76_06770"
     CDS             complement(4612..7092)
                     /gene="gyrA"
                     /locus_tag="PKK76_06770"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNL68209.1"
                     /translation="MEENPQIVRTDIQGALQKGLRIVPVEIDNQMKEAYLDYAMSVIV
                     GRALPDARDGLKPVHRRILYAMHARAWRNDRPFVKCAKIVGEVIGNFHPHGDVAVYDT
                     LVRMAQDFAMRQPLIDGQGNFGSIDGDSPAAYRYTEARLSKIASELLNDIDKSTVDFS
                     PNFDDTRQEPQVLPAAFPNLLVNGSTGIAVGMATNIPPHNLGEVIEATLALIQKPNIS
                     LEDLTKYIPAPDFPTAGIIIGGEGLKSAYLTGRGSFRIRARVDIETDKKGRDKILVTE
                     IPYQVNKKTLLEKIGDLVNNKEIEGITDILDLSDRHGIRIEIFLKKDANAQVILNQLY
                     KQTQLQISYGINMLALVDGSPKVMNLKELLQVYVKHRKEIVVRRTQHDLDEAEKRAHI
                     LEGLKIALDFIDEVIKIIRSSQTVEEARTRLMTRFKLTEIQANAILDMRLQKLTSLES
                     KKIIEELEQLRATIKDLKDILKSDDRQFKIISSELGDVKKAYPGKRKTEVDHSSVETT
                     NFNVKDLIKDEQVVISITEDGFIKRTALDTFRRQHRGGKGVKGANLKNEDMIRLVSTG
                     STHDTMLCFSNRGKIFAVPMHEVPEAGREGRGKSLKAILSLGADEKITAITSVRGFSD
                     ELAIVMISREGILKKSDLSVFASTRKGGIIAINLRKEDELINVRLVSPKDDIVIATKE
                     GSALRTNLAKMRSQGRNAAGIIGMRLEKGDEVIGMDVARKDASLMVISEKGFGKRLPF
                     NQLPAKGRGGKGMTYIKITDKNGPAMAVATVKEDDDIIVVARSGMVIRLSAKEIPALG
                     RPTVGVRVVDISEGDRVRDVAVLSDDAP"
BASE COUNT         1573 a         1852 c         1893 g         2137 t
ORIGIN      
        1 aaagaaatat cgggcagcta gtttggcgag gctcgtggtc ttccccattc ctgtgggtcc
       61 aatgagcatg actgcgcgcg cttctcctct cggaggcgca atgtctggta ctgtgcgaat
      121 cgtttccgca agtcgctcca acgctttttg ctctaccttt tgaggctgcg ccctgtcgtt
      181 ctttgagaga ttgctttcta aatgtgcgat gattgaatcg gcgtaagtgg cactcagctt
      241 cgcaccaagt aattgtgcgc ggatctttag aaaatactgt ggagtgcttt ctctctccag
      301 tttgatttct ggttttgctt cggagattgc ctgcggcgtt ggaatggctg gagttgcctg
      361 cgctaccgtt tcgggcagag actcatcttc gaaaacaggc ttcaactttc gcgcaagctc
      421 ctgtctgctg atcttcgtga attcggccgg tctcactttc ttgcttgcgg tttgttcaac
      481 ggatttctcc ggaaccatgt agtggatttc gtagatcttt cgggaaagca accccgtgcc
      541 aagtagcccg ccttctttta cttctctttg tttgatcaat acggcggctg agccatgctt
      601 ttgtctcatt tcagacatga gtctggaaat gctggggccg cgcagtgttt cgtatctcat
      661 gcgatttttc tgtattatgt cgcatttgcc gtgctggcaa ttgtttttgt agtcttgctg
      721 gatttttgcg ccttttttca gtcttttttg attgtatacc gtcgagtgga aaccaaaggt
      781 tgcgacgtgc gtttcaccct gtgccttgca gttctattct ttgctgtttc ttgcgggaag
      841 cacagacccg atccagacaa accacccctt catttgtttg tatgggggaa gaacagagga
      901 gatgtgaaaa gccttctcga gaaggaaggt tatctggttt cagagagaac cgagttcatt
      961 cgcgcgaccg taccggaatc agagggtcgc gacatcatgc cagatgaaag tccgtaccag
     1021 cttacgttct acttccagaa tgacaggctc aatattgtcc agatccagat tcgcaacagt
     1081 ccggatcgca caacgatcta tgagcaaaac ctgaagacag cctacaaatt ggctcagcct
     1141 gtcttgcaga aatcgcttcc cccgcgcaca acagaagcag gcaatcagat taaagagact
     1201 cattctcttt atgacactgg cgaagtatac atcaagctct acaaatcgca ggtcgaaatt
     1261 gcagagtctc gcatggaaga agtgcctgac gaaacagaca tcatgatcta ctccagaaag
     1321 gaaaacgaag gtatcaccgc ggaaggcctt ttgagtcctg aggaaaactg agtttcgctg
     1381 gactaccttt gtccgggtgt agaatgatga gaaaaaagga gttcatgcaa tggatctcgc
     1441 aaaagttaga gaactcctgg gtgggcaggc agagtccctc ctgggtcata aagcaaaagt
     1501 agcaaaagaa accattcacc tgcccgggcc ggatttcgta gacagaatct ggactcagtc
     1561 agacagatcg ccgcaggttt tgcgttctct ccagcagata ttctcacacg gccgcctcgg
     1621 tggtagtgga tacgttagca ttctgccggt ggatcaggga gtggagcatt ccggtggtgc
     1681 gtctttcgcg cccaatccaa tctatttcga tccagaaaac attgtaaagc tggcaatcga
     1741 aggtggctgc aatgctgtat gcagtacctt tggcgtgctc ggctcggttg cgcgcaagta
     1801 tgctcacaga attccttttc tagtgaaaat caatcataac gaatttctaa gctacccgaa
     1861 caagtacgat caagtgatgt tcggcacaat caaggaagcc tggaacatgg gtgctgttgc
     1921 cattggagca accatttatt ttggttcgga tgaaagccgt cgccagatcg ttgaggtttc
     1981 tgaagccttc gcatacgcac atgaacttgg aatggctaca gtgctctggt gctacctgcg
     2041 aaacaacgcg ttcaaggtgg atggtgtgga ttaccatact tccgcggatt taagctctca
     2101 aggaaatcac ctgggcgtaa cgatccaggc agacattatc aagcagaagc ttcctacaaa
     2161 caacggcgga tacaacgcaa ccaaacatgg caagactcat ccgaaggtgt attcagatct
     2221 ttccacggat catccgatcg atctgactcg ttatcaggtt ctgaattgct attctggccg
     2281 cgctggcttg atcaattctg gtggggaatc taagggttct tctgacctcg cagaagcggt
     2341 tggcactgca gtgatcaaca agcgcgcagg cggcatggga ttgatctctg gtcgcaaagc
     2401 gttccagcgt ccattcgagg aaggtgctca gctgctccag tctattcagg atatttacct
     2461 gaataaagat atcacggtag cctgattgga tttactcaat tcgttgctcg gggtggtcca
     2521 gaatttctct gggccgctcc cgtacctgtt tgtattcttc attctcctcc tctgtggatt
     2581 tggtcttcct cttccggaag atgtggttct ctttactgca ggtgtgcttg cctacaaggg
     2641 actcgctgac attcacataa tggtcgccgt cgcctttgcg ggcgtaatga taggagacgg
     2701 gaccatcttc tttctggggc acaagttagg cctgcgattg actcagcttc cgttctttca
     2761 acgggtgctt tctcccgtgc gattggccgc aattcgagag aggtttcatg agcacggaaa
     2821 caagatcgtc tttgccgcgc gctttatgcc tggactccgc actcccgttt tcttcacggc
     2881 agggactctg catcttccgt ttagagtatt tcttttttac gatggaatgg cagcgctcat
     2941 cagcgtgccc actatcactt acgtagtctt cttgtttgga cacgaactcg aggcagtgat
     3001 caacacggtt cgtagcgttg agcatggaat ggtcttgttg attgcccttg tgattgcttt
     3061 cttcgtagga aagtttttgt ggggcaggcg caagtctgca aatgctccgg aatgatggga
     3121 tcaagcgcgg cgcctaacag tggttaggcc agcggagcca aagaatcaat gctgtttttt
     3181 cagccggtag ggaagcacag attctccacg ccgttggctg tatatgcgct gatcgccata
     3241 tccgtgctgg cttacgcttt cactgccctg gacagcgaaa atatcatcaa atcgtttggt
     3301 ctcgtccctg cccgattctc tttcatcgat tcggtgattt caacattctt gcattccggc
     3361 atttttcacc tcattggaaa catgatcttc ttgtatatgt ttggaccagg cgttgaagac
     3421 gtggtcggcc ctttcatgtt tgttctcctt tacctgattc ttgcagtgat ttcggattct
     3481 tgctacgcat ggatgaacct gcaatcaact cttccagtcg taggtgcttc cggagctgtc
     3541 tcaggaatcc tcggggtttt cttagtattc tttccgaacg tgaaggtgaa cttgttcttg
     3601 gccgcagatt ctgaggcctc cgcatcaggt ccttttctga tgaatgcgtt ttgggccatt
     3661 ggattctggt tcgcaggtca gttactctgg gcaattctcg cacttgcttt gccaacgcac
     3721 gttgcctatg cggcgcatgt cgggggtctc ataggtggag ttattctagg tcttttgctc
     3781 aaggccgcag gttttctaga cagctttcac gccagagttc ggcggacgcg tggatcatcg
     3841 aagcccacag tttactgccc ggcgtgtgcg ttgcccattg cgcccactga atttgggcgc
     3901 tatcggtgtc ccaattgcgc gagcgagttt acttttgatg tgaagcgcgg ctatcgcctt
     3961 ttacaatagt aaaggcctgg tctcgcgcat aaccatcgtt agacgctttc caccagccgg
     4021 acattcactc tgcagtattc catccagccc agagattctg ctctttgtct ggcaatgacc
     4081 agtccagtat tctttggttt atccggcttc ttttgcagct tccagccaat ttcctgcaag
     4141 agtcgatttg cttttacgga attgcatctc ctgcacgcag ttacgagatt ccaccaggaa
     4201 ttgaaatcat agggtggtgt ttctccggta atctttttcc atctggatcg tggaatcaca
     4261 tgatctacag tcagttcagg aattggaaat cgcacgccgc agtaacagca tgtctgatcg
     4321 tccctataaa tgatgttgat cttggaagtt ttctgatcgt acctggggat cttgtgataa
     4381 cttctctgtg cgatgattcg cggcacatga agagtgagct tttcagagcg caccaggtcc
     4441 tctgattctt tgattgggat ggcgatgttg cgcagtatct ccagaatcgc ggttctggcg
     4501 ctgcaaatgt caatggcgat catgctcgta ttgagcacaa ggaccgattc ctgtaagaca
     4561 tccctcacgc gccgaaacgt aggtgcgttc cagcgcgtgt aaagaaaaaa tctaaggcgc
     4621 gtcgtcgctc agtacagcaa cgtctcgcac tcgatctccc tcagaaatgt ccacaactcg
     4681 aacgcccact gtaggccttc cgagtgccgg gatttctttg gcgctgagtc gaatcaccat
     4741 gccgcttctt gcaacgacga tgatatcatc gtcttccttc acagtcgcca cagccatggc
     4801 cggcccgttt ttgtccgtga ttttgatgta ggtcataccc ttcccaccgc gtcctttcgc
     4861 cggcagttga ttgaacggca gtctctttcc aaaacctttc tcagaaatta ccatgagaga
     4921 agcgtctttt ctggccacat ccatgccgat cacctcgtcc cctttttcca ggcgcatgcc
     4981 aatgatgcct gcggcgtttc taccctggct tctcatcttc gcgaggtttg tgcgaagggc
     5041 acttccctct ttcgtggcga tgacaatgtc gtcttttgga cttaccaggc gcacgttgat
     5101 gagttcatct tctttgcgca gattgatcgc gatgattcca cctttgcgag tggaagcaaa
     5161 aacgctgaga tccgatttct tgaggatccc ttcgcggctg atcattacga tggccagttc
     5221 gtctgagaaa ccgcgaacgc tcgtgatcgc agtgatcttt tcatctgcac ccagactgag
     5281 gattgctttg agagactttc ctctgccttc tctgcccgct tccggaactt catgcatggg
     5341 cacggcgaag attttgccgc ggtttgagaa acacagcata gtatcgtgag tggatcccgt
     5401 agacacgaga cgaatcatgt cttcgttttt caggttcgca cctttgactc ctttgcctcc
     5461 gcgatgctgg cggcggaaag tatccagagc tgtgcgcttg atgaatccat cttctgtgat
     5521 gctgatgacg acctgttcgt ctttgatcag atctttcacg ttgaagttcg tggtttctac
     5581 agagctgtga tctacttctg tcttgcgttt tccagggtaa gctttcttca catcgcccaa
     5641 ttctgaggag atgatcttga attgacgatc gtcgcttttg agaatgtctt tgaggtcttt
     5701 gattgtagcg cgaagctgtt caagttcttc aatgatcttt ttggattcga gagacgtgag
     5761 cttttgcaga cgcatatcca ggattgcatt tgcctgaatt tcggtgagct tgaagcgagt
     5821 catcagacgc gttcttgctt cttccactgt ctgcgaagag cggatgatct tgatgacttc
     5881 gtcgatgaag tccagggcga ttttcaaacc ctccaagata tgcgcgcgtt tttctgcttc
     5941 gtccaggtcg tgctgcgttc tgcgaacaac gatctctttg cggtgcttaa cgtatacctg
     6001 cagaagctct ttgaggttca ttaccttggg gctgccgtct acaagagcga gcatgttgat
     6061 gccgtagctg atctgcagct gtgtttgctt gtagagctgg tttaggatta cctgagcgtt
     6121 tgcatctttt ttgaggaaga tttcgattcg gattccatga cgatccgaca gatcgagaat
     6181 atccgtgatc ccttcaattt ccttgttgtt aacaagatct ccaatctttt cgagtagagt
     6241 cttcttgttc acctgataag gaatttccgt cacgagaatt ttatcgcgac ccttcttatc
     6301 cgtttcaata tcaacccggg cgcgaattct gaatgaaccg cggcctgtca gataggcaga
     6361 tttcaatcct tctccgccga tgatgatgcc ggcagttgga aaatccggag ctggaatgta
     6421 ctttgtcaga tcttccaggc taatgtttgg tttttgaatc agcgcgagag tagcttcgat
     6481 cacctcaccg aggttatgcg gagggatgtt cgtggccatg cccactgcaa ttccagtgga
     6541 accgttgacc agaaggttgg ggaaagcagc agggagaacc tgcggttcct gtcttgtgtc
     6601 gtcaaagttg gggctgaagt caaccgtgct cttatcaatg tcgttgagca gttctgaagc
     6661 gatcttggag aggcgcgctt ccgtataacg gtaagcagcc ggactgtctc catcaatcga
     6721 tccgaaattc ccctgaccgt cgatgagagg ctgtcgcatg gcgaaatcct gtgccatgcg
     6781 cacgagagta tcgtacacgg ccacgtcacc gtgcggatga aagttaccaa tgacctcacc
     6841 tacgatcttt gcgcacttga cgaatggcct gtcattgcgc caggcgcgcg cgtgcatcgc
     6901 atagaggatg cggcggtgca cgggcttgag accgtctcga gcatccggaa gtgctctgcc
     6961 cacaattacg ctcattgcgt aatcgaggta tgcctctttc atctggttat cgatttctac
     7021 cggaacaata cgaagacctt tctgcagcgc accttgaatg tctgtgcgaa cgatttgtgg
     7081 attttcctcc atccttcttg cccttcttgt ttttttggcg tttccgcgct gtttggtgcg
     7141 cggtggcggc cgtttagcag ggctcaacct ggaaggcggg cggctggtct tcttaggaac
     7201 cgggcgcttt ttcgctttgc cagggcgcgc cgcttttggc gcttttttta cctgcttttt
     7261 acctttatct gttttggatt tggaacgaaa accgcttttc ttggccatta tgtcctcttg
     7321 gggggcaaag ggcattttga ggccttccag tcaaaggaaa acggagaaag gcggcaaaac
     7381 cgggtaagaa aaggccgcgg acggaagcac ttgacaccgt gcgtccccca ctcctcctgc
     7441 aaatatgcga attgg
//
LOCUS       DAORFF010000126         7439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4968_length_7439_cov_3.551192, whole genome shotgun sequence.
ACCESSION   DAORFF010000126 DAORFF010000000
VERSION     DAORFF010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4968_length_7439_cov_3.551192"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..470)
                     /gene="cutA"
                     /locus_tag="PKK76_06775"
     CDS             complement(150..470)
                     /gene="cutA"
                     /locus_tag="PKK76_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNL68210.1"
                     /translation="MTPLFVYITTSGPEEARKIGTALVEMRLAACVNILPPVESIYHW
                     KGAIARETETVLIAKTRQDLFGRLSETVRSLHSYTNPCIVGLPIKEGSAEYLQWIADE
                     TASA"
     gene            526..1284
                     /gene="fabG"
                     /locus_tag="PKK76_06780"
     CDS             526..1284
                     /gene="fabG"
                     /locus_tag="PKK76_06780"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNL68211.1"
                     /translation="MEKPLKGKIALVTGSARGIGRAIAEDLAEWGADQVILDMNQEAC
                     EKTAAEIAGKYGVRAIAVACNITKKSDVEAAFSKIQKEYGTLHFLVNNAGVLKDNLLL
                     RMKEEEWDMVLGVNLKGAFLMTQVMLKMLMKAEGGSRIVNISSVSGLAGQIGQANYSS
                     SKAGLIGLTKVTAREYGSRNVLCNAVCPGYVQTDMTGALPPEVQEMLRKSVPLGRPGQ
                     VKDISRVVRFLCSDDASFISGTIIRVDGGMATGM"
     gene            complement(1298..2566)
                     /locus_tag="PKK76_06785"
     CDS             complement(1298..2566)
                     /locus_tag="PKK76_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68212.1"
                     /translation="MGHGPHNCPGKQAGKHFPVLVYFLGLFTSGLQALPLLPDASCDN
                     HGGMEARSSHDSRAGLTVRMECFGWQLAGSLSGRDHSTTGDSIIGSIRAESNVATIIA
                     GRRDLPALHSGYLTDEVSVLVSPLRKSSVFVRPVPSLWVPGIFAVEGMTGPGIFFLSP
                     DGRVFAALHPDGSCGLTVDYQTGARVFVDAERLRFGEKLPWTWEGVAYASFGGVNIET
                     SRQRKWDFDEKGRFDESRKGRSGLASIVLGPDWLHVEAAGMDRYKTSMRMAGATAACI
                     PDILSFGPVIRGRSYRVRTYGSSSEVTDSAFAGGLALQTQFVQLEALYESRRGAASSG
                     ELSLSIERKNLSASLSGFAKCGRKIEGRSLRVLRTRPEYETGTTFYLEETSALTLRVR
                     SGFLYLSAGFSRSKNKTNRYGTAEFKSEFF"
     gene            2625..3125
                     /locus_tag="PKK76_06790"
     CDS             2625..3125
                     /locus_tag="PKK76_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNL68213.1"
                     /translation="MSDRLRDLYSANRRDLYTYLSRMSGDADLALDLLQESFLNYFEH
                     YRGKEMPDDLSSRRILFRIARNLFINHFRSKASQTTHLEEQYLEAAPAQNAGPDLTFA
                     IQKLIQGLPEKERTAIVMRYLESMRLEDIGAVLGLSVSAVSRLVSKSEKMLEWEAKKA
                     GISLDA"
     gene            3206..4195
                     /locus_tag="PKK76_06795"
     CDS             3206..4195
                     /locus_tag="PKK76_06795"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNL68214.1"
                     /translation="MENKRSSLARALNTSDALPDFNPEWLTRSPRLRADASVFAERTN
                     RMPYYAAAAAVLFALLGTSLYLRSPAPAAQVASRGLVISVAGPTQVQRGEATTDLYNG
                     DMITEGDRITTGKDARVEIALAGHSVRILENSSVTLLELKKTSEGQRNVGLDVAQGTL
                     LSSVKRLSKQESFEVRTPVAIAGVRGTRFSVAVNDSGSQVRLYDGAVVVKRGESETVL
                     SPGKGVEVTKSGVSELTEDSTDYAQQIQEMETNPNADPEIIAAADSLKSAKSEKELKA
                     LYQKIEIIRLRDGRVIRGVIAQQVGSRVVIHTSTGMHVVQSSEIEDQSFAEEP"
     gene            4246..5349
                     /locus_tag="PKK76_06800"
     CDS             4246..5349
                     /locus_tag="PKK76_06800"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNL68215.1"
                     /translation="MKTRIKFLAIALIASAATLAVNCGQKASEAANTSMMVVYTTGSA
                     FILPEGKSEVPAKVGMVVRQNDVIRTESGTVDLQTRSGSAVRVREMTRITISALNASG
                     ETKLNMQHGGLLASVKKAGQGENFSVVTPTAIAGVRGTTFSVEVGEDSRSSVRVIDGK
                     VAMAPRVASLDKFSDAQIKADPNLQKLSDLSKKEIVLEDKTQGTLDANLEKQLMNANQ
                     VLDSASPAPAKLTDVVAATSTAKPVESQKVEVSTREIIEARTLVTVDPSIMEKAVKGQ
                     ESEAAKEITSRREVAQETVLNQIMEDAGSVKLNSEKEIQQRYNKLELVVLKNGDQIRG
                     AVIAQTENSIVVHSPEGVKKIPRSDLNYQEPLF"
     gene            5463..5657
                     /locus_tag="PKK76_06805"
     CDS             5463..5657
                     /locus_tag="PKK76_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020780474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNL68216.1"
                     /translation="MRPRKVCVCRSVTETEIRESVRNGHHTFEAVQEETDCSTGCGTC
                     ESQVRSIIANELSALQAAKK"
     gene            5694..6308
                     /locus_tag="PKK76_06810"
     CDS             5694..6308
                     /locus_tag="PKK76_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNL68217.1"
                     /translation="MFSLQWSDLIRAGGPTLFVLLICFIATCILIIERSAYFRARARE
                     TAAVLEQLRAQEGSTVLPRDSYKMVNSPVTSVLYDCYPYAGGSLFEEVKSRAIAENLP
                     SIERYLGVLATLGTVSPYIGLLGTVFGIIRAFASLGSGPAADAAMSGLNAGIAESLVA
                     TAAGLFVAIPATISFNLFRRSADDLVTQLEIAASRLRTLLERSK"
     gene            6308..6709
                     /locus_tag="PKK76_06815"
     CDS             6308..6709
                     /locus_tag="PKK76_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNL68218.1"
                     /translation="MVHYRANKTQLFSGINIVPFTDVVLVLLIVFMISAPGLLNTAMN
                     IKLPGSTQATKRSDTKITVGLDASGKIFVNNEVVAREKVQEKVKELLKGESGEILLNA
                     DMKSHHGEVVELLDLLRQSGGNNVFIGTVRK"
     gene            6716..6946
                     /locus_tag="PKK76_06820"
     CDS             6716..6946
                     /locus_tag="PKK76_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68219.1"
                     /translation="MLKRSRRLPEAISILAFLLPLPLLFPLYPLSMFVLIGSLFETAL
                     SAAVSALLLREQIHPAQRKKAELNLLRKKFSK"
     gene            6943..7131
                     /locus_tag="PKK76_06825"
     CDS             6943..7131
                     /locus_tag="PKK76_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68220.1"
                     /translation="MKNDLLDRQIAFKGDMKKLLTPEQYEKWEAKREAKKEKMKDHFK
                     ERKGDGHKKHKPELIEEN"
     gene            7202..>7439
                     /locus_tag="PKK76_06830"
     CDS             7202..>7439
                     /locus_tag="PKK76_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68221.1"
                     /translation="MKKIIAVVALLFAFSISANAQEKKVTVKEVAAKQTLTPEAAGKN
                     DAKALADLVKLDEKEFQIFSDLFQTKHRILAEQGL"
BASE COUNT         1985 a         1772 c         1896 g         1786 t
ORIGIN      
        1 gtaaatatcg cagccgcgga agcaggcctt tcgctcagca ccactccgaa gtccagtgtg
       61 ttgtctttga ttccggcatt ccagccggcg gcaagaaagc cgcgtggaaa attgctcaca
      121 ggaaacagaa gtctgcgccg gacagcatgt caagcggatg cggtttcatc cgcaatccac
      181 tgcagatatt ctgcggaccc ttccttgatc ggaagaccta caatgcaggg gtttgtgtag
      241 ctatgaaggg accgcaccgt ttcgctcaat cgcccgaaaa gatcctgacg agtctttgcg
      301 ataaggactg tttctgtttc gcgtgcgatc gcacccttcc agtgatagat cgactccaca
      361 ggtggcagta tgttcacgca ggctgccagg cgcatctcca caagggctgt gccgattttt
      421 ctcgcttcct ctggtcccga tgttgtgatg taaacaaaga gcggtgtcat ctgctgattc
      481 gaatatcgct tgatacacag gcaacctgga ttcgatctgc tttctatgga aaaaccactc
      541 aaaggtaaga ttgctctggt cacaggatct gcgcgcggga ttggccgtgc tatcgcagaa
      601 gatctggcag aatggggcgc agatcaagtc atcctggata tgaaccagga agcatgcgaa
      661 aagactgctg cggaaatcgc aggtaagtat ggcgtgcgag cgatcgccgt tgcttgcaac
      721 atcacaaaga agtcagatgt ggaagcagct ttctcgaaga ttcaaaaaga gtatggcaca
      781 cttcattttc ttgtgaacaa cgcaggcgta ctcaaagaca atctgcttct tcgcatgaag
      841 gaagaagaat gggatatggt acttggcgtg aacctgaagg gagcatttct gatgactcag
      901 gtgatgctca agatgctcat gaaggcagaa ggcggatcac gcatcgtcaa tatcagttct
      961 gtctctgggc tggcgggtca gattggccag gccaactatt cttcgagcaa agcaggtctc
     1021 attggtctga caaaggtgac tgcgcgtgag tatggttcgc gcaatgtgct gtgcaatgct
     1081 gtttgtcctg gttatgtgca aacagacatg acaggcgctt tgccgccaga agtacaggaa
     1141 atgctgcgca agtcagttcc actgggaagg ccaggtcagg tgaaagacat ttctcgcgtg
     1201 gttcgttttc tatgttcaga tgatgccagt ttcatcagcg gaacgatcat acgcgtagac
     1261 ggcggcatgg ccactggcat gtaagagaat ctcgaattca aaaaaattct gatttgaact
     1321 cagccgttcc gtagcggttg gttttatttt tggaacgaga aaacccggcg ctcaagtaca
     1381 gaaatccgct tctcactctc agagtgagcg cggatgtttc ttctaaatag aaggtggtgc
     1441 ctgtttcgta ttctggtctg gtccgaagca cccgtagact tctcccttct atctttcgtc
     1501 cgcattttgc aaaacccgac aggctagcgc tcaggttttt tctttctata gaaagactca
     1561 gttctccaga tgaagcggct cctcttcgcg attcgtaaag tgcttccaat tgaacgaact
     1621 gcgtttgcaa ggccagtccg ccggcaaatg ctgagtctgt gacttcagac gacgatccat
     1681 acgttctgac tctataggat cttccccgaa tcactggacc aaatgacaga atgtccggaa
     1741 tgcatgcggc ggttgctcct gccattctca tggatgtttt gtaacgatcc attcctgcag
     1801 cttccacgtg caaccagtcc ggtccaagca caatcgacgc gagtccggat cttcctttgc
     1861 gcgattcatc gaacctgcct ttctcatcga aatcccattt cctctgacgc gatgtttcta
     1921 tgttcactcc accaaacgac gcgtatgcga cgccttccca ggtccacggc agtttttccc
     1981 cgaagcgcaa cctctccgcg tccacaaaga cgcgcgctcc cgtctggtag tctacagtga
     2041 gcccacaact accatccggg tgaagcgcgg caaacacgcg cccgtctgga ctgagaaaga
     2101 atatgcctgg tccggtcatc ccttcgactg cgaagattcc gggcacccac agggaaggaa
     2161 ccggtcgaac aaagacggat gattttctca gaggtgagac aagaacagac acctcgtctg
     2221 taagatagcc cgaatgaaga gcgggcagat ctcgtcttcc ggctatgatg gtggccacgt
     2281 tggattctgc ccggatcgat cctatgatgg agtccccggt ggtagagtga tcgcgaccgc
     2341 tgagcgatcc tgccagctgc cagccgaaac attccatgcg gacggtcagg ccggccctcg
     2401 agtcgtggct cgatcgcgct tccattccgc cgtggttgtc gcagcttgca tccggaagga
     2461 ggggaagcgc ctggaggcca gacgtaaaaa gcccgaggaa atacaccaga acaggaaaat
     2521 gctttccagc ctgcttcccg gggcaattgt gcggaccgtg acccatcgca atccagtagg
     2581 taggccattc aagaattgtt ccggcttatt tcgagtaggc aatcttgtct gatcgtctgc
     2641 gtgacctcta ttctgcgaat cggcgggatc tatacacgta tctgagccgc atgtcaggcg
     2701 acgcagatct ggccctggat ctactccagg agtcgttcct caactatttc gagcactatc
     2761 gcggcaaaga aatgccggac gacttgtcca gcaggcgaat tctatttcgc atcgcgcgaa
     2821 atctattcat caatcatttt agaagcaagg caagccagac cactcatctg gaagaacagt
     2881 atctggaagc cgcacctgcg cagaatgctg gacccgacct tacatttgct attcaaaagc
     2941 tcatccaggg ccttccggaa aaggaacgca cagcgatcgt tatgcgatat ctagaatcca
     3001 tgcgattgga agacattggc gcagttctcg ggctttctgt ttccgccgta tccagactcg
     3061 taagcaagtc tgagaagatg ctcgaatggg aggccaaaaa ggccgggatc tctctggatg
     3121 cgtaactgga cgcgaaaatc atcgcttttg agacaaaaaa agagcaaaaa aattctgaca
     3181 cgttgttaga gtagggatgg gagacatgga gaacaaacgg tccagtctgg ctcgcgcgct
     3241 aaatactagc gatgctctcc cagactttaa tccagagtgg ctcaccaggt cgccacgtct
     3301 tcgcgcagat gcatctgtct tcgcagagcg caccaatcgc atgccatact acgcggccgc
     3361 agccgctgtc ctctttgcct tgctaggtac ttctctttat cttcgctctc ctgctccggc
     3421 cgcccaggtg gcttccagag ggttagtgat tagtgtggcc ggtcctactc aagtgcaaag
     3481 gggagaggcc accacggact tatacaatgg agatatgatc acggaagggg atcgcatcac
     3541 gacaggtaaa gatgcgagag tagaaattgc actcgcaggt cattcagttc gcattcttga
     3601 aaatagttct gtaacccttc ttgaactcaa gaagacatcg gaaggccagc gtaacgtcgg
     3661 attggatgta gcgcagggaa ctctactgag ctcagtgaaa cgattgagca aacaggagtc
     3721 cttcgaagtg cgcacaccgg tcgccattgc gggcgtgcgc ggcaccaggt tctctgtggc
     3781 tgtaaacgat tctggttctc aggtcagact ttatgacggc gctgtagttg tgaagcgtgg
     3841 agaatccgag accgttcttt ctccagggaa aggagtggaa gtcaccaagt caggtgtgtc
     3901 ggaactgaca gaggattcca ctgactatgc gcagcaaatt caggaaatgg aaacaaatcc
     3961 aaatgcggat cctgaaatca ttgcagctgc agattctctc aagtctgcga agtctgaaaa
     4021 agaattgaaa gcattgtacc aaaagatcga gatcatacgt ctaagagacg ggagagtcat
     4081 ccgcggtgtg attgcgcagc aagtcggatc cagggtggtc atccatactt ctacgggaat
     4141 gcatgtagtg caatcatcgg aaattgaaga ccagagtttc gcggaggaac catgaacagt
     4201 attatagtta aaacgccggt atgtccggta aaaggagacc caacaatgaa gacccgaatc
     4261 aagtttctag ccatcgcgct gatcgcatct gcggcaacgc tggcagttaa ctgcgggcag
     4321 aaggcgtctg aggcggccaa cacttccatg atggttgtgt ataccaccgg aagcgcattt
     4381 attctgcccg aaggaaagtc cgaagttccg gccaaggtag gaatggttgt tcgtcagaat
     4441 gacgttattc gtaccgaatc gggaactgtg gacctccaga caagaagcgg aagcgcagtg
     4501 cgcgtacgcg aaatgacgag aatcacaatc tccgctctca acgcgagcgg cgaaactaag
     4561 ctgaacatgc agcatggcgg tttgcttgct tctgtaaaga aagcaggaca gggagaaaac
     4621 ttctccgttg taacaccgac cgcgatcgct ggtgtgcgcg gaacaacttt ctcagttgaa
     4681 gtaggcgaag acagccgttc ttccgttcga gtaatcgacg gtaaagtggc catggctcct
     4741 cgcgtagctt ctctggacaa gttcagcgat gcgcaaatca aagctgaccc gaatctccag
     4801 aaactgtctg acctgagcaa gaaagaaatt gttctggaag acaagactca aggaacgctt
     4861 gatgcgaacc tcgaaaagca gctgatgaac gcgaatcaag ttcttgattc agcatctcca
     4921 gctcctgcaa aactcacaga cgttgtggct gctacaagca ccgcaaagcc agttgaatcg
     4981 cagaaagtag aagtaagcac acgtgagatc attgaagctc gcactctggt aacagtggat
     5041 ccatctatca tggaaaaagc tgtaaaaggc caggaaagcg aagctgcaaa agaaatcaca
     5101 tccaggcgtg aagttgctca ggaaaccgta ctcaaccaga tcatggaaga tgcgggaagc
     5161 gtgaagctga actctgaaaa agagatccag cagcgttaca acaagcttga gcttgttgtc
     5221 ctcaagaatg gtgaccagat tcgcggcgct gtgattgctc agactgagaa ttccatcgtc
     5281 gtgcattcac cagaaggcgt gaagaagatc ccaagatcag acctgaacta tcaggaacca
     5341 ctgttctaag atcagaattt tcacgggagc tgattcgtcg gctcccaaaa aacttctcga
     5401 aaagcgcccc ccggggcgct ttttttgttt aaatggacga tttgtcagca gtgtatgcaa
     5461 tgatgcggcc gcggaaggtt tgcgtctgca ggtcagtcac ggaaacagaa atcagagaat
     5521 cggttagaaa cgggcaccac acgtttgagg ccgttcaaga agaaacagat tgttccaccg
     5581 ggtgcggaac ctgtgaaagc caggtgagat ccatcattgc aaacgagctg agtgcgcttc
     5641 aagctgcaaa aaaatgaatt tccacacgca ggcatgatct agaattcgcg tatatgttct
     5701 cactgcaatg gtctgacttg attcgtgctg gtggacctac cttattcgtc ttactcattt
     5761 gttttattgc aacttgcatc ctgatcattg aacgttctgc ctattttcgc gcacgcgcaa
     5821 gagagactgc tgccgttctc gaacagttgc gcgctcagga aggatctacc gttcttccgc
     5881 gagacagtta caagatggtg aattctccgg ttacttctgt cctgtatgat tgttatcctt
     5941 atgcaggtgg atctcttttc gaggaagtca agagtcgtgc gatcgcagaa aaccttccct
     6001 cgattgaaag atacctcggg gttcttgcta ctctaggaac agtcagtccg tacattggac
     6061 ttctcggaac tgtatttgga atcatccgcg ctttcgcaag cctgggaagt ggccccgcgg
     6121 cagatgctgc tatgtcgggc ttgaatgcgg gcattgccga atcactcgtt gccactgcag
     6181 ccggtctctt tgttgctatt ccggccacga tttccttcaa tctgtttcgc cgctctgcgg
     6241 atgatcttgt gactcagctt gagattgctg cctctcgcct tcgcacctta cttgagagga
     6301 gtaagtagtg gttcattacc gcgcgaataa gacgcaactt ttttccggga tcaacattgt
     6361 tccattcacg gatgtggttc tggttttgct catcgtgttt atgatttcgg cgcccgggct
     6421 tctgaacact gcgatgaaca tcaaattgcc tggctccact caggccacga aaagatcgga
     6481 tactaaaatc actgttgggc tggatgcgtc cggaaagata tttgtgaaca acgaagtagt
     6541 ggctcgcgag aaggttcagg aaaaagtcaa ggaactactc aaaggagagt ccggagaaat
     6601 cttgctcaac gctgatatga aatcacatca tggcgaagta gtagaacttc tggatttgct
     6661 cagacagtct ggtggaaaca atgttttcat cggaacagtg cgaaagtagg ttcgcgtgct
     6721 gaagagatcg cgccggctac cggaagccat ttccattcta gcttttctac ttccattgcc
     6781 tcttctgttt ccattgtatc cgctcagtat gttcgtattg ataggatctt tgttcgaaac
     6841 tgctctttct gcggctgtgt ctgcgctgct gctcagagag cagattcatc cggcgcaaag
     6901 aaagaaagcg gagttaaacc tactcaggaa gaaattttca aaatgaaaaa tgatttactt
     6961 gatagacaaa ttgctttcaa aggagatatg aaaaaacttc ttactccaga acaatatgaa
     7021 aagtgggaag caaaacgtga agccaaaaaa gaaaaaatga aagatcattt taaagagcga
     7081 aaaggtgatg gtcataaaaa gcataaacct gaattaattg aagaaaatta aaaataaatt
     7141 ttgagttgta ttaaaaaaaa agtagttttg gataattaat tcaactctaa actataaaaa
     7201 aatgaaaaaa ataattgcag tagtagcctt gttatttgct ttcagcatat cagcaaatgc
     7261 tcaagaaaag aaagttactg ttaaagaagt agctgcaaaa caaacattaa ctccagaagc
     7321 agcaggaaaa aatgatgcaa aagctttggc tgatttagta aaattagatg aaaaggagtt
     7381 tcaaatattt agtgatttgt ttcaaactaa gcatagaatt ttagctgagc aaggtcttt
//
LOCUS       DAORFF010000127         7415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_4998_length_7415_cov_3.615625, whole genome shotgun sequence.
ACCESSION   DAORFF010000127 DAORFF010000000
VERSION     DAORFF010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7415
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4998_length_7415_cov_3.615625"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1192
                     /gene="bioB"
                     /locus_tag="PKK76_06835"
     CDS             98..1192
                     /gene="bioB"
                     /locus_tag="PKK76_06835"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNL68222.1"
                     /translation="MDLRERLKRVANGESMPVEADLALALRGEIPLSELLGIAEEPRR
                     KYFGNKIQVHILDNVRNGYCAEDCGYCAQRKTATEEIPAYSLKKEQEILKEAEVAFRS
                     GAYRFCIVTSGTGPSEKQTVQYADLIRKIKEKYPMRLCLSAGIIKDPHFAQILAEAGL
                     DRYNHNLNTSESHTAEIVTTHTFQDRIQTIENVRDAGISVCSGLIAGMGESPEDLAQV
                     AVRLRSLNVPSIPVNFFLPVPGHNVAHASTLSPDYCLRILCVYRIANPSAEIRIAAGR
                     EIHLKDKQQEAFRVGNSLFVSGYLNVKGSDARETYNLIHNAGFEIDADTSELDPEFRS
                     LIMGEQNSPAGAQSGDLRMKTREDLRSFAE"
     gene            complement(1204..1875)
                     /locus_tag="PKK76_06840"
     CDS             complement(1204..1875)
                     /locus_tag="PKK76_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68223.1"
                     /translation="MLEEVSKLIDSRETAGSLEKAIELLEKLKAENPEKDIIRGKLSS
                     AFFYKGLFSPEGTREREDAFDNGVKYGQEAITLNPRAVYGNFWYASNLGYLGMCRGAL
                     ASLASIDPFKKSMEVVLKENENFYFGGPHRAIGRLYHQAPGWPISIGNKNKAFEHLEK
                     CVQLAPNFMNNRLYLAELLIDMGKKDKAVEHLNFCKDTALNPGHEKEDGVYKEQAEKL
                     LKRIS"
     gene            1964..3238
                     /locus_tag="PKK76_06845"
     CDS             1964..3238
                     /locus_tag="PKK76_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNL68224.1"
                     /translation="MQPFIAPGFNLPLAWTADLLFPVLIKAVQNIDDVEIRPEDKEML
                     RTLRNERLIFFTNHPTTAEPPIAYYIGNLMGSRFRYMASRQVFDWTFGLTGKIISNLG
                     AFSVIAGISDRDSLKTARSSLAQAGGKLVLFPEGEPTSGENDSLMPFQPGLAQLGFWA
                     LEDARKVDPAADIVVLPGFIKYIYKGTPSQLQEHLEESMAALEKKLGVDPGGRNLLRR
                     FLHFGKVLLERAEQQYGVEVSPDKDFDYRIGRIRHTILDNIADRFNVKNFDRKADAIG
                     KLRQLFALMEMISIGYPDPNLPKVTPEDLEWAHGECVKAFDFIVIKKDYLVSNPTPER
                     FYEWLARFESYVYGKKPRALGGEPSPRARKAVVKFAKPFRLSEFAPKDKKAKKSALDE
                     LLKKLRREMQEMLDESQSLTRPLFKPGDIGGE"
     gene            3267..3554
                     /locus_tag="PKK76_06850"
     CDS             3267..3554
                     /locus_tag="PKK76_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HNL68225.1"
                     /translation="MYTFRAKVWIYSGQGAWHFVTLPAKTARELKEDYGHSRRGWGTI
                     KVTASTGKTKWNTSVFPEKKSGSFVLPIKMEVRKAEKISAGKTIQVHLEIA"
     gene            3650..4234
                     /locus_tag="PKK76_06855"
     CDS             3650..4234
                     /locus_tag="PKK76_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68226.1"
                     /translation="MIAGHYAAAFLPAAHKARGPFWLFLLASQIPEFLWLLFAIAGIE
                     PTLPASILDATFDSIVVDMRFSHNLVPALFQAIITGVVVAIVFRSAVTALWSAALVVI
                     HVLCDYVVGFSHQIMWYSSPSIGLNSYKTMPYVAIGIELVFAWICLGYFYWKRKEDLP
                     WSNRQWMILTLAFSAGILAWMPAARIPIRHWLGL"
     gene            4275..6350
                     /locus_tag="PKK76_06860"
     CDS             4275..6350
                     /locus_tag="PKK76_06860"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL68227.1"
                     /translation="MRQIARQLILIAALIATAGCSHIFPPQGPIQLNESTPMFRPGPQ
                     MEYCVDESGTKTLEEIKNCNFIPVRAYYPAFGITTNAVWVRFRILPDPKATTSWYFEL
                     ASSLPRSVHFYRLHQDGTLADKLIYDEVPPRLRLIPHHYFLFPIQNVTEPESIYMRAQ
                     SVGGLNMPVFIWNARSFPMRDMLRTGFFAMFFGLMFAMALYNLLLFMSIRDRSYLYYV
                     FYILFVTEYFAFISGHAAYLVPDSWTGKTPALGPVVALAATIFALIFSRKFLLLPEKR
                     PRLAMAANVVALIHLLGLIALPFLTIPQMAQIGNGMPVLGIIVILVAAISLVRDGFKP
                     AKYFLTAWSFLMIGILLFIVQNLNFIPAGFITQYGQFFGAAFEAILLSLALGYRINSL
                     RQSEQEARRDLSQEQARALAEQTAAAESFARFVPGEFLEYLGKKNIMSISHGDAVRKQ
                     MAVLFLDIRGFTEMSERLGSEGTFSFLNEFHGGLEPIIQKHDGFIDKFIGDAIMALFP
                     APTGAVQAAIEMQNWVAYTGKARVGIGIHFGELMLGTIGSPHRLETTVIGDTVNLASR
                     IESINKQYGTEIIVSDSVYKLIQEIPQLAAREVDAIRVRGKKQPVVLYEIMNGLSPDV
                     LASREKHISSYMGALLSYRAGDFAAAKTQFSEHMQNVNGDSVAEMYLKRLDRINESAP
                     WDGIWEAEH"
     gene            complement(6368..>7415)
                     /locus_tag="PKK76_06865"
     CDS             complement(6368..>7415)
                     /locus_tag="PKK76_06865"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNL68228.1"
                     /translation="CKYPSHCEGVFCSIWPAALVFARPRTLPDTGQTTCATTAGAVIS
                     CAGTGQDAEYATPGAYILYETTLGNAVGEVLGSDLLWQKCSFGQTGNSCAGTALSTVT
                     RTQAFAACSGVAIADRVWRLPSVREMHLLPYFGGATVAMPSSFFPNTASGTNVYHWTS
                     TTVVASPANSYLFAQKFGFFASQSNALATDTFARCVAGPAQPTQDYVRLGVTEPSVRD
                     NTTGLTWTVCALKTGGAADTSANCAGPAPANFTQTNALTFCESLVYGGFTDWRLPNVR
                     ELASLMDYTQSVNPTVNQTYFTANPSATVYWSSTNDISNAANAYIFDVSDGTSGTIAR
                     TASIGDVRCVRGPW"
BASE COUNT         1897 a         1881 c         1816 g         1821 t
ORIGIN      
        1 tatctcgcac tttctatgcg tgataaaacg cgcgaggggg gcttgttgcc gctctcgaaa
       61 acgcttgttc gcctggtcgt cagttttttt ctgaaacatg gatctgcgag aaagactgaa
      121 gcgcgttgca aacggggaga gtatgcccgt agaagcggat ctagcacttg ctttacgcgg
      181 agaaattcca ctctccgaat tgcttgggat cgcagaagag ccgcggcgca aatactttgg
      241 aaacaagatc caggtgcaca ttctggacaa cgttcgaaac ggatattgcg cggaagactg
      301 cggatattgc gcgcagagaa agactgcaac agaagaaatt cctgcctatt ctttgaagaa
      361 agaacaagaa atactgaagg aagctgaggt agcgtttcgc tctggcgcct atcgcttctg
      421 cattgtcacg tccggcactg gtccctcgga aaaacaaact gtgcagtacg cagatctgat
      481 tcgcaaaatc aaagagaaat atccgatgcg tctctgcttg agcgcaggca tcatcaaaga
      541 tcctcacttc gcacagatac ttgcggaagc gggtctggat cgatacaatc acaatctaaa
      601 cacttcagaa tcgcatacag cggaaatcgt aaccacgcac acatttcagg atcgcattca
      661 gacgatcgag aatgtaaggg atgctggaat ttctgtgtgc agtggactca ttgcaggaat
      721 gggcgaatcc ccggaagatc tcgcgcaagt tgcggtcagg ctacgaagtc tgaatgtgcc
      781 ttctattccg gtgaatttct ttcttccggt gcccggccac aacgtcgcac atgcgtccac
      841 tctttccccg gattattgtc ttcgcattct ctgtgtctat cgtatagcaa atccatccgc
      901 ggagattcgc attgcagccg gaagagaaat ccacctcaaa gacaagcagc aggaagcatt
      961 tcgcgtaggg aattctctgt ttgtttcggg ctatctgaat gtgaagggat cggatgcgcg
     1021 tgaaacatac aatttgattc acaacgcagg atttgaaata gatgcagaca catccgaact
     1081 ggatcctgaa ttccgcagct tgatcatggg agaacaaaac agtccagccg gagcgcagtc
     1141 aggcgatctt cgcatgaaaa cacgcgaaga ccttcgcagc ttcgcagagt agaaaaacct
     1201 gaattaagaa attcttttca gaagcttctc tgcctgttcc ttgtatacgc cgtcttcttt
     1261 ttcgtgacca gggttgagcg ctgtatcctt acaaaagttt aagtgttcca ctgccttgtc
     1321 tttcttcccc atgtcgatga gaagctccgc cagatagagg cgattattca tgaagttagg
     1381 cgctagttga acgcactttt caagatgctc gaatgctttg ttcttatttc caatgctgat
     1441 gggccagcct ggcgcctggt ggtacaaacg accgatcgcg cgatgaggtc caccaaagta
     1501 aaagttctca ttctctttca agacaacttc catggatttc ttgaaaggat caatggatgc
     1561 cagagaagca agcgccccgc gacacatccc caggtaacca agattcgaag cataccagaa
     1621 atttccatac acagcacgcg gattcagcgt gattgcttcc tggccatatt taacgccgtt
     1681 atcaaatgca tcttctcgtt cgcgcgttcc ttccggcgag aacagtcctt tatagaagaa
     1741 agcactggag agcttccccc ggatgatgtc tttttccgga ttctccgcct taagtttttc
     1801 caacaattcg atggctttct caagcgatcc cgcagtttcg cgtgaatcaa tcagtttgct
     1861 tacttcttcc agcatgacta tggactgaac caatgccgaa aatttgaaag ttaaaaaaac
     1921 ttactttacc aaaaaaagga agggggctac ctctgaaccc cttatgcaac cattcatcgc
     1981 gcccggtttc aatctacctc tcgcctggac agcggatttg ctgtttcccg ttctaatcaa
     2041 ggcggtgcag aatatagacg atgtggaaat tcgcccggaa gacaaagaga tgcttcgcac
     2101 cctgcgaaac gaacgcttga tattttttac aaaccatccc acgactgcgg aaccacccat
     2161 cgcatactac attggaaatc tcatgggctc ccggtttcgt tatatggcat cgcgtcaggt
     2221 gttcgactgg acattcggtc tgactggcaa aatcatttca aacctgggtg cattctcagt
     2281 cattgcaggc atcagtgacc gcgactctct caagaccgct cgctcttctc tcgcacaggc
     2341 agggggaaaa cttgtgcttt tccccgaagg agaaccaacc agcggcgaaa acgacagtct
     2401 catgccattt cagccaggtc tcgctcagct cggtttctgg gctctagaag acgcgcgcaa
     2461 agtggatccg gccgcggaca ttgtagtact tcctggattc atcaagtaca tctataaagg
     2521 aactccttcg caattacagg agcatctcga agaatccatg gcagctcttg aaaagaaact
     2581 cggagtggat ccgggtggaa gaaatctgct tcgccgattc ttgcatttcg gaaaagttct
     2641 actcgagcgc gcggagcaac agtacggagt ggaagtttct ccggacaaag atttcgacta
     2701 tcgcattgga agaattcgcc acacgattct ggacaacatt gcagatcgat tcaacgttaa
     2761 gaactttgat cgcaaagcgg atgcaatcgg aaagctgcgt cagctattcg cgctcatgga
     2821 aatgatatcc attggttatc cagatcctaa tcttcctaaa gtaaccccgg aagatctgga
     2881 atgggcacat ggtgaatgcg taaaggcttt cgacttcatc gtcatcaaga aagactacct
     2941 ggtttctaac cctacaccgg agcgttttta tgaatggctg gcacgatttg aatcctacgt
     3001 ctacggcaaa aagccgcgcg cactcggagg agagccatct ccgcgcgcta gaaaggcggt
     3061 cgtgaaattt gcaaaaccgt tccgcctctc ggaattcgcg ccaaaagaca agaaggcaaa
     3121 gaaatctgct ctagacgaat tgctcaagaa gctccgccgg gaaatgcaag aaatgctcga
     3181 tgaatcacag tcattgaccc gtcctctttt caagccagga gacattggag gagagtgacg
     3241 gataggatca acccttcgcg ccagttatgt acacatttcg cgcgaaagta tggatctact
     3301 ccgggcaggg agcgtggcat tttgttacac tccctgcaaa gacagccaga gaactgaaag
     3361 aagactacgg ccacagcagg cgcggatggg gaaccatcaa agtgactgcg tcgacgggaa
     3421 aaacaaagtg gaacacatct gtctttccag agaaaaagag tggatcgttc gttctgccca
     3481 ttaaaatgga agtgcgcaaa gcggaaaaaa tctccgctgg caaaaccatt caggtacatt
     3541 tagaaatcgc ttaacggtaa ggagcatcca tgacgtccct catttttgcg ctcattgcgt
     3601 tctacgcgtt ctttttctgg ctgcttatgc ggcttgcaag gcggagcgta tgatcgccgg
     3661 tcactacgcg gctgcttttc ttcccgcagc tcacaaagca cgaggaccgt tctggctttt
     3721 cttgcttgct tctcagattc ctgaatttct ctggctgctt tttgcgatag ccggcattga
     3781 acccacgctc cctgcatcca ttctggatgc aacgtttgat agcattgtag tagatatgag
     3841 gttcagtcac aacctggttc cggccctatt ccaggcaatc atcactggag tcgtggtggc
     3901 cattgtgttt cgaagcgcag tcactgctct ctggagcgcc gctctcgtcg tcatacatgt
     3961 gctatgcgac tatgtggtcg ggttttcgca tcagatcatg tggtattctt ctccatccat
     4021 tggtctaaac agctacaaga caatgcccta cgtggccatt ggaatcgagc tggtgttcgc
     4081 ctggatttgc cttggttact tctactggaa aagaaaggaa gacctcccat ggtcgaacag
     4141 gcagtggatg attctgactc ttgcttttag cgcgggcatt ctggcgtgga tgccggctgc
     4201 tcgaatcccc attcgacact ggcttggtct gtagaagtca tttgacgaac acccacagcc
     4261 gtttgtactg cgacgtgcga cagattgcca ggcaactgat tctcatcgcg gcgttgattg
     4321 cgacagccgg ctgctcacat atctttccgc cgcagggccc cattcaattg aatgaaagta
     4381 cgcccatgtt tagacccggg ccacagatgg aatattgcgt ggacgaatct ggcacaaaaa
     4441 ctctggaaga gataaagaat tgcaacttta ttcctgtgcg cgcttactac ccggctttcg
     4501 gaatcacaac caacgcagtc tgggtccgct ttcgcattct tccggatcca aaagcaacca
     4561 ccagttggta ctttgaactg gccagttcac ttccgcgctc agttcatttc tatcgacttc
     4621 accaggatgg gacccttgca gataagctca tttacgatga agttcctccc aggctcagac
     4681 tcattccgca tcattacttt ctgtttccca tccagaacgt aacagaacca gagtccatct
     4741 acatgcgcgc gcagtctgtg ggcggactca atatgccggt cttcatatgg aatgccagat
     4801 cctttccaat gcgcgacatg ctacgcactg gtttttttgc catgttcttt ggcctcatgt
     4861 ttgccatggc actctacaac ctcttgctat ttatgtccat tcgagacaga agctatctct
     4921 actatgtctt ttacattttg ttcgtaacag agtattttgc attcatcagc ggtcatgcgg
     4981 catacctggt gcccgattca tggactggaa aaacaccggc gcttggaccg gttgtggcgc
     5041 tggctgctac catattcgct ctcatattct cgcgaaaatt tctgctcctg ccggaaaaga
     5101 ggcctcgcct ggcaatggcg gctaacgttg tggctttgat tcaccttctg ggtttgatcg
     5161 cccttccatt cttgacgatt ccccagatgg cgcagattgg caacggaatg ccagtcctcg
     5221 ggatcattgt gattctggtt gctgcgatca gcctggtgcg agatggattt aagccagcca
     5281 aatacttcct tactgcatgg tcgttcttga tgattggaat tttgcttttc atcgtgcaaa
     5341 atctaaactt cattcctgca ggcttcatca ctcaatatgg acaatttttt ggagccgcgt
     5401 ttgaagcgat ccttttgagt ctggcgctgg gatacagaat caactcattg cgacaatcgg
     5461 aacaggaagc cagaagggat ctgtcgcaag aacaggccag agctctcgca gaacagaccg
     5521 ccgcggcaga gtcatttgcg agatttgtac caggagaatt tctggaatac cttggaaaaa
     5581 agaacatcat gagcatttcg catggcgacg cagttcgcaa gcaaatggca gttctgtttc
     5641 tggacatacg agggttcacc gaaatgagcg aacggctggg ctcagaagga acgttttcgt
     5701 ttctaaatga atttcacggc ggactggaac caatcattca aaagcacgac ggattcatcg
     5761 acaagttcat cggggacgcc atcatggcac tcttcccggc accaaccgga gcggtccagg
     5821 ccgccatcga aatgcaaaac tgggttgcat acacgggcaa agctagagtt ggaattggaa
     5881 ttcatttcgg agaattgatg cttggaacca ttggctctcc tcacaggctg gaaacaactg
     5941 tgattggcga tacagtcaat ctggcttcca gaatagagag catcaataag cagtacggta
     6001 cagaaatcat tgtatccgat tcagtctata aactcattca ggaaattccg cagcttgctg
     6061 cgcgcgaagt cgacgcgatt cgtgtacgag gaaaaaaaca acccgttgta ttgtatgaaa
     6121 tcatgaatgg tctctcacct gacgttctgg cttcgcgcga aaaacatatc tcttcttata
     6181 tgggagcttt actctcctat cgcgccggtg atttcgcggc cgcaaaaact caattcagtg
     6241 aacacatgca gaacgtcaat ggcgacagtg tggctgagat gtatctcaaa cgcctcgatc
     6301 gaatcaatga atcagcgcca tgggatggaa tctgggaagc ggaacattga tcaacggcgg
     6361 agaagactca ccacgggcct cgtacgcatc gaacatctcc gatcgaagcc gtacgggcga
     6421 ttgtgccgga agtaccgtct gaaacatcaa agatgtaagc gttcgcagca ttgctaatat
     6481 cgttggtgga agaccaataa actgtagcac tcggattggc ggtaaagtag gtctgattta
     6541 ctgttgggtt cacagactgc gtgtaatcca tcaagcttgc gagctcccga acattcggaa
     6601 gtctccagtc agtaaatcca ccgtacacga gcgactcgca aaatgtgaga gcgttcgtct
     6661 gtgtgaagtt agccggcgcg ggacccgcgc aattagctga agtgtctgcc gcgccaccgg
     6721 tcttgagagc gcaaaccgtc caggtcaagc ccgtagtatt gtcacgcaca gaaggctcag
     6781 ttacaccaag cctcacataa tcctgcgtgg gctgggcggg accggccaca caacgagcga
     6841 acgtgtctgt tgccagcgca ttggattgcg aagcgaagaa accaaatttt tgagcaaaca
     6901 gatatgagtt tgcggggctg gccacaacag ttgtagaagt ccaatgatac acgttcgttc
     6961 cggaagctgt gttcggaaag aaactagaag gcatggctac tgttgcacct ccaaagtaag
     7021 gaagaagatg catctctctt acactcggca aacgccacac acgatcagca atcgcaactc
     7081 cggaacatgc ggcgaatgcc tgcgtacgag tcaccgtaga caacgcagtg ccagcgcaag
     7141 aatttccggt ttgcccaaaa ctacatttct gccagagaag atctgaaccg agcacctctc
     7201 ctactgcatt tccgagagtt gtttcgtaga gtatgtaggc ccctggcgtt gcgtattcgg
     7261 cgtcctgccc tgtacccgcg caggagatca cagcccccgc ggtcgtcgca catgtggtct
     7321 gtccagtatc gggaagagtt cgcggacgcg caaagacaag ggctgcgggc catatactac
     7381 aaaagacgcc ttcgcaatgc gaaggatact tacag
//
LOCUS       DAORFF010000128         7377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5047_length_7377_cov_4.100792, whole genome shotgun sequence.
ACCESSION   DAORFF010000128 DAORFF010000000
VERSION     DAORFF010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5047_length_7377_cov_4.100792"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PKK76_06870"
     CDS             complement(<1..719)
                     /locus_tag="PKK76_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68229.1"
                     /translation="MADRYAFLDLLKEQEALARGANLNDKVHHAIYKTIKSNSTVDGV
                     RHNTLYHLGVNVPEVLHLTKKDLVTVLTRLLQERLVTQIFVFLFEPGVPKPSFRPCFV
                     TIPPEEKQSVGQLYQEGIVQSSRALESYIDTLPPIGADAIWEDLEKDLEMEQVPQPSD
                     LPASILDLFSGVHASTFDVVPAPEMIQATLGDIQEQLNKKQKLVQVIDYGFMPLRDRE
                     ILDRVESAGDFLLSKVIPKYKG"
     gene            complement(721..1476)
                     /locus_tag="PKK76_06875"
     CDS             complement(721..1476)
                     /locus_tag="PKK76_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNL68230.1"
                     /translation="MGRRPRKAFENREFLLSPAARKVRILTEVTYAEHVFAEQGVTDT
                     VVFFGSARVPAPEQKKEGKINEFGVYYDRARDLSREITSWSMAHAAPAKRNFVICTGG
                     GPGIMEAANRGALDAGGKSIGLNIELPEEQFPNPYITDELNFDFHYFFTRKFWFLYYA
                     RALVVFPGGFGTFDELFETLTLQQMKSIRNTIPVFLYGKDFWRRVIDFDFLAEIGMIA
                     RKDLELFHMVDSVEETMAHIVPALEESLRNPPE"
     gene            1491..2615
                     /locus_tag="PKK76_06880"
     CDS             1491..2615
                     /locus_tag="PKK76_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNL68231.1"
                     /translation="MKIGVDARPLAYAGNGNARYLFDMLRGAESGHDFVLYSHRPIHS
                     DYRSTLDRLGVSFRIDQSWTSRAGLLFLNFRLPGWLKEDLCDVYWGSLSMLPFFYKQR
                     VQIPSMVNFHDLNAFVAADTMTKANALQHGLTNGHTLRNADRVLCLSQTTANDIARFF
                     PAVKNKLEVVYPGIELKPIRPIAPEGTPGKLRHFYLMVGTIEPRKNQNTVIDAYLKLK
                     QELTSKHKLPPLVIVGRKGWGADVLFERLSSGILEKDGIYYIAGASDAVLEWCYKNSS
                     LYLFPSVHEGFGLPILEAAFRGIPLALSDIPIFREVGGKSAFAAPFDIQAWQEILRKP
                     PKKAPAFNKKVWSIENRAKKITQIVREIAEIRTTGRKQDS"
     gene            complement(2612..2863)
                     /locus_tag="PKK76_06885"
     CDS             complement(2612..2863)
                     /locus_tag="PKK76_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68232.1"
                     /translation="MTYVSLEMLYLEREPGGRGPFRYNDEHDVIRRITVESCSGSCVC
                     RLKYSDPDLDEIFYDSAGCRKQSRYSNHPLYFRGSGCRI"
     gene            complement(3040..4164)
                     /locus_tag="PKK76_06890"
     CDS             complement(3040..4164)
                     /locus_tag="PKK76_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68233.1"
                     /translation="MRSKSNRLADFLKDKEQVRKFKESIRRIEFNAIVRPGSHSSSYR
                     KEYLEKTAGRLFPYPFLDHDFHIKCIADHVERAVSGEIVLHPVLAYASLFDLRSSYAP
                     ASKSKAKGKKKTSRAAARTRGKSGDALRKKRFLAVQKIMSRLRVPAYMKRAKTEFEIK
                     ESSVRAFEDMYGEFLNLRLRIQTLENEVLAYQNPDYAHPPIKAKLQEFQTQNNDLAMR
                     FVKEQNRADILESEIQRLTDHILAQPAEGELDSLKREYNVLSQKCDNLVSRNIELSNR
                     MKELGRSKQLEDILDGIRDKINSALRAGIHEAPDAILKSLSDEISQLKRARLYLGRAL
                     YDIGILYHRKGDRERAKEELRAARELGVRDADVENILKQN"
     gene            complement(4220..>7377)
                     /gene="metH"
                     /locus_tag="PKK76_06895"
     CDS             complement(4220..>7377)
                     /gene="metH"
                     /locus_tag="PKK76_06895"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNL68234.1"
                     /translation="SDILLVETIFDTLNCKAAIYAIDQYVRESGNNIPVMLSGTIVDM
                     SGRTLSGQTTEAFWLSISHAKNLLSVGLNCALGSAQMRPFLTELSRVATVPVSLYPNA
                     GLPNAFGGYDESPEYMAQQIDAYAGEGMINIIGGCCGTTPDHVKAMAQAALKHPPRKP
                     AQTSGEFALAGLEPLTLRSNMNFVNIGERTNVTGSPKFAKLILEGNYDEALSVARQQV
                     ENGAQIIDINMDEGMLDSEAAMTKFLNLVAAEPDIAKVPVMVDSSKWSVLESGLKCLQ
                     GKSVVNSISLKEGEENFLNASRKIRSYGAATVVMAFDENGQADSYERRIEICKRAYDL
                     LVKDGFPPEDIIFDPNILTVGTGIAEHNNYAVDFIRATEWIKKNLPHARVSGGISNIS
                     FSFRGNNPVREAMHTAFLYHAVRAGLDMGIVNAGMLGVYEDIPKDLLEMVEDVLLNRR
                     PDATERLVEFADRFKGKASAEVKTDEWRNTSVEERLKHALVHGILDFIDQDTAEAHAK
                     YGKPLSVIEGPLMGGMSVVGDLFGAGKMFLPQVVKSARVMKKSVAYLIPFMEKEAAEG
                     GGRKAAAKVLLATVKGDVHDIGKNIVGVVLACNNFEVVDMGVMVPRDKILDQAREIGA
                     DVIGLSGLITPSLDEMVEVAREMERRGLKVPLLIGGATTSKIHTAVKIAPSYRQPVIH
                     VLDASRSVPVVNSLITPDQSAGFASSIEAEYEKLRHDFANREQSKKFLTLENARRNAP
                     RIAHTGNPEPKRKSIQVLRNYPLDELRQYIDWTPFFITWEMQGKYPAIFQDEKFGTEA
                     KKLFDDANALLDQIIARKLIHAHGVAMIWPANSVGDDIEVYADESRTSTIAVFHGLRQ
                     QTEKRAGEPNRCLSDYVAPRGVKDYVGAFAVTAGDGVLDLAKSYEDKGDDYNSIMVKA
                     IADRLAEAFAERLHEMVRKEIWGYASDESLSAEDLVREAYQGIRPAPGYPSQPDHTEK
                     QDLFRLLEATKHTGVSLTESLAMSPAASVCGIYLAEKQAEYFNLGKIEKDQVEDYARR
                     KGMPVGEVEKWLSPALNYDRP"
BASE COUNT         1759 a         1972 c         1741 g         1905 t
ORIGIN      
        1 cccttatact ttgggatcac tttagataga agaaaatccc ctgcagactc tacgcggtcc
       61 agaatttcgc ggtctcgcaa gggcatgaat ccatagtcaa ttacctgcac gagtttttgc
      121 tttttgttca attgctcctg gatgtctccg agagtggcct gaatcatttc aggagcaggc
      181 acaacatcaa aagtggacgc atgcactccg gagaaaagat ccaggatgct tgccggaaga
      241 tccgaaggct gcggcacttg ctccatttcc agatccttct ccagatcttc ccagattgca
      301 tctgcaccaa tgggaggcag agtatcaatg taagattcca gggcgcgcga tgattgaaca
      361 attccctcct ggtaaagctg gcctacggac tgcttctctt ccggcggtat cgtgacaaaa
      421 caagggcgaa acgatggctt tggaactcct ggctcaaaga gaaatacgaa gatctgcgtg
      481 accaatcgtt cttgcagaag acgagtcaga actgtaacca gatccttctt tgtcagatga
      541 agcacttctg gaacgttcac acccagatgg tataaagtgt tgtgcctcac tccgtctact
      601 gtggagttcg atttgattgt tttgtaaatg gcatggtgta ccttatcatt gaggtttgca
      661 ccacgcgcca gggcctcctg ttctttcagg agatcaagaa aggcgtaacg atccgccatg
      721 ctactccgga ggattcctca gagattcttc cagggcaggt acaatgtgcg ccatcgtttc
      781 ttctacagaa tccaccatgt gaaataactc gagatccttt ctggcaatca tgccaatctc
      841 agcaagaaaa tcaaaatcta tgactcggcg ccagaaatct ttgccgtaca ggaatactgg
      901 aatcgtattt cgtatactct tcatttgctg gagagtcagg gtttcaaaga gctcgtcaaa
      961 tgtgccaaac ccgcccggaa aaacaaccag ggcacgagca tagtagagaa accagaattt
     1021 gcgggtgaag aaataatgaa aatcgaagtt caattcatct gtgatgtatg gatttggaaa
     1081 ttgctcttcg ggcaactcaa tgttcagtcc aatgctcttt ccgcctgcgt ccagggcgcc
     1141 tctattcgca gcctccatga tgcctggacc tccacccgta cagatgacga agttcctctt
     1201 tgccggagca gcatgagcca tggaccagga tgtgatttcg cgcgaaaggt ctcgggcgcg
     1261 gtcgtaataa acaccaaact cgttgatctt cccttccttc ttttgttcgg gcgccgggac
     1321 tctggcggat ccaaaaaaga ccaccgtgtc cgtaacaccc tgctctgcga acacgtgctc
     1381 ggcgtaggtg acctccgtca gaatccgaac cttgcgggca gcgggtgaaa gtagaaattc
     1441 gcgattctca aatgccttgc gcggtcgcct gcccatgaaa agttctcctg atgaaaattg
     1501 gagtcgatgc ccggccactg gcctatgccg ggaatggcaa cgcccgctat ctgtttgata
     1561 tgctcagggg cgccgaatcc gggcacgact ttgtgctcta ttcgcacaga cccatacact
     1621 ccgattatcg gtccactctg gaccgattgg gagtcagttt ccgcatcgac cagagctgga
     1681 cttcgcgagc ggggctgctt tttctgaatt ttcgtcttcc tggctggctc aaggaagatc
     1741 tgtgcgatgt atactggggc agtctgtcca tgcttccctt cttctataag cagagagtgc
     1801 agatcccgtc catggtcaat ttccacgacc tcaacgcatt cgtagccgca gataccatga
     1861 caaaggcgaa cgcactccag cacggcctga ccaacggaca cactctgaga aacgcagaca
     1921 gggtgctctg cctgtcccag accacggcaa acgacatcgc acgttttttc cctgcggtca
     1981 aaaacaaact cgaagttgtt tatcccggga tcgaactcaa accaatcagg cccattgcac
     2041 ccgaaggcac tccgggaaag cttcgccatt tctatctcat ggtgggaacc attgaaccgc
     2101 gaaagaacca gaatacagtc atagatgcat atttgaaact caaacaggaa ctgacatcga
     2161 aacacaaact cccgcctctc gtgatcgtcg gaaggaaggg ctggggcgcg gatgttctct
     2221 ttgagagact ttcttccggg attctcgaaa aagacggaat ctactacatt gccggggcaa
     2281 gcgacgctgt actggaatgg tgctacaaga attcgtctct ctatctattt ccaagcgttc
     2341 atgaaggatt tggacttcca atcctcgaag ctgcatttcg cggcatcccg cttgctctat
     2401 ccgacatacc tatctttcgc gaagtaggag gaaagtccgc attcgccgca ccattcgata
     2461 ttcaggcgtg gcaagaaatc cttcgaaaac ccccgaaaaa agcccctgct tttaataaga
     2521 aggtctggtc gattgagaac cgggccaaaa aaatcacgca gatcgtgcgc gaaatcgcag
     2581 aaatcaggac tactggcaga aagcaggact cttaaattct gcagccgctc ccgcgaaagt
     2641 agagagggtg attcgagtat ctggactgct tacgacagcc ggcagaatcg tagaagatct
     2701 cgtccaggtc cgggtctgag tatttcaatc tacaaacgca ggatccactg cagctttcta
     2761 ccgttatgcg gcgaatgaca tcgtgctcat cattgtacct gaaaggacct cttcctccag
     2821 gctctctttc gagatagagc atctcaaggg aaacgtacgt cattctatcg aggaaaatgg
     2881 ctctgggagt atcccctgcc tcgcaggaac ccttgactga gttgaattca atgtcacaaa
     2941 tgaacgattt tcgatctgca gagagagtgc agagcttgcc tggaggtgca gtcttctcct
     3001 gcttcgcgca agcgaagata cagaagaaaa gaaccaggat cagttttgct ttaatatgtt
     3061 ctccacgtct gcgtcacgga ctccgagttc tcttgctgcg cgtaattctt ctttggctct
     3121 ttccctgtcc ccttttctgt ggtagagaat accaatgtca tagagggcac ggcctagata
     3181 gaggcgagcg cgcttgagct gtgaaatctc atcgctcaga ctcttaagaa tcgcatccgg
     3241 tgcttcatga atgcctgctc tcagagcgct gttgattttg tctctgatcc catcgagaat
     3301 atcttcgagt tgcttggatc gtccaagctc tttcatgcga ttggacagct caatgttgcg
     3361 gcttacgagg ttatcgcatt tttgagaaag aacgttgtat tctcttttca gcgaatccag
     3421 ttctccttcc gcgggttgag ccagtatatg atccgtcagt ctctggatct cgctttctag
     3481 aatgtccgcg cggttttgtt cttttacaaa acgcatggcc agatcattgt tctgagtctg
     3541 aaattcctgg agctttgctt tgatgggcgg atgagcgtag tctggatttt gataggccag
     3601 cacttcgttt tctagagttt gaatccgcag acgaaggttc aaaaactctc cgtacatgtc
     3661 ttcgaatgcc cgaacggaag attctttaat ttcaaattct gttttcgcac gcttcatgta
     3721 ggcaggcacg cgaagtctgg acatgatctt ctgaactgcc agaaagcgtt tcttacgaag
     3781 agcgtccccg gacttgcctc tggtgcgagc tgccgcacgc gaggtctttt tcttgccttt
     3841 tgccttagac ttactcgcag gcgcataaga acttctcaag tcaaagaggc tggcatacgc
     3901 aagaacagga tgcagaacga tttctccgct gactgcacgc tccacatgat ctgcgatgca
     3961 tttaatatgg aagtcatggt ccaggaatgg ataggggaag agtctacctg ctgtcttttc
     4021 gaggtattcc tttctataag aagacgaatg gctccccgga cgcacgattg cgttgaactc
     4081 aatcctgcgt atggattcct tgaacttccg gacctgctct ttgtccttca ggaagtctgc
     4141 cagtctgttg ctcttgctcc tcaaaaccct tgctccgatt agattatcgg aggctctcaa
     4201 aggcacggct acctcttttt taaggtcgat cgtagttcag ggccggggaa agccattttt
     4261 ctacttcgcc cacaggcatg ccctttctgc gggcataatc ctcaacctgg tccttttcta
     4321 ttttgcccag attgaagtat tctgcctgtt tttcggccag ataaatgccg caaacggacg
     4381 cagcaggaga catggccagg ctttccgtca aactgacgcc agtatgtttt gtagcctcga
     4441 ggaggcggaa cagatcctgc ttctccgtat gatccggctg agacggataa ccaggcgccg
     4501 gccgaatccc ctgataggct tctcggacca gatcttccgc ggacagagat tcatccgaag
     4561 catagcccca gatttccttt ctcaccattt catggagtct ctctgcgaat gcttctgcaa
     4621 gccgatccgc aattgctttt accatgatgg agttataatc atcccctttg tcttcgtatg
     4681 attttgcaag atcgagaacg ccgtcccctg cagtcacggc gaatgcacct acataatcct
     4741 tcacgcctcg cggagcgaca taatcggaga gacaacgatt gggctctcct gccctctttt
     4801 cagtctgctg gcgcaggcca tgaaaaaccg caatcgtaga agtgcgcgat tcatccgcat
     4861 acacttctat atcatctccc accgagttgg caggccagat catcgcaact ccgtgcgcgt
     4921 gaatgagctt tcgcgcaatg atctgatcga gcaatgcgtt ggcgtcatca aaaagcttct
     4981 ttgcctcggt gccaaacttc tcatcctgaa agatcgcagg atatttcccc tgcatttccc
     5041 aggtaatgaa gaaaggagtc cagtcaatgt attgcctgag ttcatcgagt ggatagtttc
     5101 tgagaacctg aatgctcttt cttttgggtt caggatttcc agtatgcgca attctgggcg
     5161 cattcctgcg agcattctcc agcgtgagaa atttcttact ctgttcgcga ttggcaaagt
     5221 catggcggag cttttcgtac tcggcttcga tggaagatgc aaaacccgca ctctggtcag
     5281 gagtgatgag actgtttaca actggcacac tacgcgatgc atcgagcacg tgaatgactg
     5341 gctgcctgta ggaaggagca atcttgactg cagtgtgaat cttggaagta gtggcgccgc
     5401 caatcaagag cggaactttg agtccgcgtc tttccatttc gcgcgctact tccaccatct
     5461 cgtccaatga cggagtgatc aacccggaca gaccaattac atccgcgccg atttcgcgag
     5521 cctgatccag aatcttgtcc cggggaacca tcacacccat gtcgacgact tcgaagttgt
     5581 tgcacgcaag tacgactcca acgatgtttt tgccaatatc gtggacatca cctttgactg
     5641 ttgcaagaag tactttggcc gcagctttcc tgcctccgcc ttccgcggct tctttctcca
     5701 tgaaaggaat caggtaggcc acagattttt tcatcactct ggcactcttc accacttgcg
     5761 gcaaaaacat tttgcccgca ccaaacaggt ctcccactac agacattcca cccatgagag
     5821 gcccttctat cacagacagt ggtttgccgt acttcgcatg cgcctccgcc gtatcctggt
     5881 cgatgaaatc caggatgcca tgcaccagtg catgtttgag tctttcttct acagatgtat
     5941 ttctccattc atccgtcttc acctcagcgg acgctttgcc cttgaacctg tctgcgaatt
     6001 caaccaggcg ttctgttgca tccggcctgc gatttaaaag aacatcctct accatctcca
     6061 gtagatcttt tgggatatcc tcgtaaactc caagcatccc cgcgttcacg atccccatgt
     6121 cgagccccgc gcgcactgca tgataaagaa acgcagtgtg cattgcttcg cgcactggat
     6181 tgtttccgcg aaaagagaaa gaaatattgc taatacctcc gctcactcgc gcatgcggaa
     6241 gattcttctt gatccactcg gtcgcgcgaa tgaaatccac agcgtagttg ttgtgttctg
     6301 cgatcccggt tccaacagtc agaatgttcg gatcgaaaat aatgtcctct ggcggaaatc
     6361 cgtcttttac aagcaaatca taggcccttt tgcagatttc aatccggcgc tcgtatgaat
     6421 ctgcctgacc gttctcgtca aacgccatta ctacagtagc cgcaccatag gagcgaatct
     6481 tgcgcgatgc gttcaggaaa ttctcttcgc cttctttgag cgaaatagaa ttcacaacac
     6541 tttttccctg caggcatttc aagccagact caagtacact ccatttggaa gagtccacca
     6601 tgaccggaac tttcgcaatg tccggctctg ccgcgaccag attcaaaaac tttgtcatgg
     6661 ccgcttctga atcgagcatg ccttcatcca tgttgatgtc tatgatctgc gctccgttct
     6721 ccacctgctg gcgggcaacg ctgagtgctt cgtcgtagtt tccttccagg atcagcttcg
     6781 cgaactttgg agaccctgtg acgtttgtac gttcgccaat gtttacaaag ttcatgttgc
     6841 tgcgaagggt aagaggctca agaccggcca gcgcaaactc accgctcgtt tgcgcaggct
     6901 tgcgcggagg atgcttgagc gccgcttgcg ccatcgcctt gacatgatcg ggtgtagtcc
     6961 cgcagcaacc gccgatgatg ttgatcatcc cttccccggc atacgcatca atctgctgtg
     7021 ccatatattc tggactttca tcatacccgc cgaatgcatt gggaagtccc gcgtttggat
     7081 aaagacttac cggaaccgtt gccacgcgcg aaagttccgt gagaaacggc ctcatctgcg
     7141 cagaaccaag agcacagttg agccccactg aaagcagatt tttcgcatgt gaaatagaaa
     7201 gccagaatgc ttccgtagtc tgcccggaga gagttctccc tgacatatcc acaatggttc
     7261 cagaaagcat gaccggaata ttgttaccgc tttctcgcac atactgatcg atcgcataga
     7321 tcgcagcttt gcagttcaaa gtatcaaaaa tagtttctac aagtagaatg tcagagc
//
LOCUS       DAORFF010000129         7280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5160_length_7280_cov_3.207612, whole genome shotgun sequence.
ACCESSION   DAORFF010000129 DAORFF010000000
VERSION     DAORFF010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5160_length_7280_cov_3.207612"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /gene="fliM"
                     /locus_tag="PKK76_06900"
     CDS             <1..733
                     /gene="fliM"
                     /locus_tag="PKK76_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNL68235.1"
                     /translation="NPTTLAVINMDPLKGSAVLEIDPGITFTIIDRLFGGPGEPTKIT
                     RELSDIEMSVMENIIVRILGNLREAWATVIDLRPRLGNIETNPQFAQIVPPNDMVVLI
                     TLETKVGDVEGMTNLCIPYITIEPIISKLSAQYWYSSIRRGESDENRAIIQERLDMVD
                     IPLRCEVGEVDLPMADVINVREGDVVRLGGTHIKSDMMIRVGDRKKFKCRPGRIGNRL
                     CVQIGDAIEDVPDELLGSSRSEDEV"
     gene            complement(730..1212)
                     /locus_tag="PKK76_06905"
     CDS             complement(730..1212)
                     /locus_tag="PKK76_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68236.1"
                     /translation="MRAKMKTRPGILIDEKDRWIYQQAELIQPDILAYFRQNLHRSDR
                     TYFIRNTFGELVEEGELDAVLGFPLHAISAVPQTEDVHFLVDSGERITIPFSAIYLDR
                     DDCFFFEMPGRGVPVRLTASAMTHVSDTLVDTSPPRWSAGVPVSRKSLSEYFINPPAR
                     "
     gene            complement(1197..1598)
                     /locus_tag="PKK76_06910"
     CDS             complement(1197..1598)
                     /locus_tag="PKK76_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68237.1"
                     /translation="MRRKILFAAIFALASCRSVEFIPDAEYANRRKDNPESFHLYARK
                     PDVKVEALGRLIFRNFYGNPGSEEFQRDVALEAAARGANCGYVSKKQINHQTQFRTNA
                     IDSQNKHGGVTGEVQAEIETVEVTLFQCERK"
     gene            1620..1868
                     /locus_tag="PKK76_06915"
     CDS             1620..1868
                     /locus_tag="PKK76_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68238.1"
                     /translation="MAKKGSKESSQGIITYIRTLAPLAYDVFTAWRDRPKWEQIMADL
                     ELVAQENEKLRKRSVLLTVFLGALFLWNAVLTYFLFAR"
     gene            1925..2632
                     /locus_tag="PKK76_06920"
     CDS             1925..2632
                     /locus_tag="PKK76_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68239.1"
                     /translation="MLDDSQQDEASKEEIRKKENPLRIGVCSLDELEEKVKAFRIMNQ
                     TALKKRYVLSREDIITSAGETIVQKRSEVDISRAKLMRRHFQGDKILKTFQPDEGIVI
                     ISDMSTAEGIPLSMDLVTQVMNLGGGAYEAFIDRVDFFSDFLNLLKKALFPRLVIIGY
                     LPGDRLDVEQMNFLRARKIDQYMRVIELTHTAFKPKPYFTKVKHIVIDISEPKTWGRF
                     IIELVREYTKPYLFEEF"
     gene            2632..3351
                     /locus_tag="PKK76_06925"
     CDS             2632..3351
                     /locus_tag="PKK76_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL68240.1"
                     /translation="MFLNLLFFLLLSPDRAREMYRTSLKLDDVRAVELLARAYEEDKT
                     NQDVTYRLGYLYHKMNRTREAVDYYSKTVRLNSCHERALNNLGTIAYQEKKKQEARSY
                     YEKAAKCGKDAVTPLYNLANLESEEGNEEAARSLYERVVRKDPNHARSRHNLGLLYFR
                     RYNQTGSDVDLEAAEKQLKEAVRLSPSDPLTLFNFALVLEAGQKTDEAIRVLKKADSL
                     SSGKTIQPRIREALARLKGKG"
     gene            complement(3358..5805)
                     /locus_tag="PKK76_06930"
     CDS             complement(3358..5805)
                     /locus_tag="PKK76_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNL68241.1"
                     /translation="MALDPAKALQQAKIPAGTVLFEAGKAATMLWMVHEGEVTLREPS
                     GRKLYNLGPSSVPGFSALMLQKPYAASYTASKDTVISAFPVNPAGNYNSLVLGKLNVG
                     IAAARSMVQEAILSRKTFVAASEFGGEIQKAIDNLSLAYYKLNPGAFQQAPGGGGQVL
                     DPVVPQTRVLLEEFQSSGGEIPDIITAGWLEQDHSSLLKKHYEMESEFDEEEFTFLRR
                     ILSLPADLQGNMFKADPALLQIISAKLATIVYAGIGELRQIEETILESTNAVLSGEYS
                     FIEKFFLVADVQGEEGRPNVIGVVKFLVTFAEKLKSRHESMFGKPIGSIAASLDNLRE
                     MVKTQKPEEKAQEATAAAVAAGVDIQALKKELAGSIGKILNFVQLPAEDGRKLLADLK
                     TLKSFNNPLDSGGDPRKVRRAINKTYWSIYEKTFFKMKETKILPLPVDWMLRFGFFDE
                     EMLDDEHIAQLTQCKDSTRGKPGYSVLSALDWLPYVGDKKEPPSLDEMGMGFFEKLKQ
                     EHKDKGWKKETDVPDEYAGYAIRVRYEILNFLEINVRLTSGSPATSFPILTRYQINTP
                     LDKAFVTADKLSECIDKILAIDYSLFHRETLFNDESRGILKEFIQLQVIPYFIIVPSI
                     GTKVMMWQDLASPNKRTSKGRIAIPVFATADLFSIMVDAMAAFRWELTKSIQGFEWNN
                     VAVPSITADYTDYVQFYKKNRELSPEIKEKLASEFKRFRSDRDRFANDYSNWLKGEAE
                     GNMKMNRVVRAIFYRHVPFAKGVRDKVATQPAFAELHNRFVNIRTKKLREFEMKYRKY
                     SEPLPDVLQKNLDFYRV"
     gene            complement(5852..6514)
                     /locus_tag="PKK76_06935"
     CDS             complement(5852..6514)
                     /locus_tag="PKK76_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNL68242.1"
                     /translation="MSLQNLERLKARLEQLGHPEVRVVAISKTHPAEKITELLRAGHR
                     DFGENRFAEARDKIPAVDTTGLAPEQMPIYHHIGPLQSGNARQISGLFQVVHGASSLS
                     GIQALNKACHGRSESLRYFIQLNLTGEQSKMGGMTIEELDSLGEPPAGPGFEFAGLMT
                     MGPSDSEPKKTREVFRSLRRIRDERFSGMELSMGMSQDYEIALEEGSTLLRIGSLIFG
                     SR"
     gene            complement(6495..6977)
                     /locus_tag="PKK76_06940"
     CDS             complement(6495..6977)
                     /locus_tag="PKK76_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68243.1"
                     /translation="MQISGKYLLSSAETLLNRRSNRIASQGTGPDTSRELQPALMHSR
                     LMQIQGHLAHVQHDYSREQARLASVDAVEGRENLQFDGAPLFPEKDTGDLRDKITDRL
                     DVLGKQLKSLQVEMENMVALGFKPEEYKIDPKLAHDTSMRPIDPDRVARLTRSVPSES
                     "
BASE COUNT         1692 a         1777 c         1881 g         1930 t
ORIGIN      
        1 gaatcctacc acacttgctg taatcaacat ggatccattg aagggttcag ccgttcttga
       61 aatcgacccg ggcatcacat ttaccatcat agaccgattg tttggtggtc caggggaacc
      121 tacgaagatt acgcgtgaac tttcagacat cgagatgtct gtaatggaaa atatcattgt
      181 gcgcatcctc ggaaacctca gggaagcgtg ggccaccgta atcgatctta gacccagact
      241 cggaaatatt gaaacaaatc ctcaattcgc gcagattgta ccgcccaatg acatggtggt
      301 tttgatcaca ctggaaacaa aggtgggaga cgtagaggga atgaccaacc tctgtatccc
      361 atacattaca atcgaaccaa tcatttcaaa actgagtgcg cagtactggt attcatccat
      421 tcgccgcggt gagtcagatg aaaacagggc catcatccag gaacgtctgg atatggtgga
      481 cattcccttg cgctgcgaag ttggagaagt ggatcttcct atggcggacg tcatcaatgt
      541 acgcgaaggg gatgtggttc gcctcggtgg cactcacatc aaatcagaca tgatgattcg
      601 ggtgggagat cgcaagaagt tcaaatgccg tccaggtcga atcgggaatc gtctgtgtgt
      661 tcagatcggg gatgcaattg aggacgtgcc ggacgaacta ctaggatcaa gtcgttcaga
      721 agacgaagtc taacgcgccg gcggatttat aaaatattcc gaaagggact ttcgagagac
      781 cggaacgcct gcgctccagc gcggtgggct tgtgtctacg agagtatctg atacgtgagt
      841 catggcgctt gcggtcaggc gaaccggaac tcctcttcca ggcatttcaa agaagaagca
      901 atcgtcccga tccagataaa tggcagaaaa ggggatggtg attctctcgc cggagtccac
      961 aaggaaatgt acatcttctg tttgcgggac agcggatatt gcgtgaagag gaaatccaag
     1021 aaccgcgtcg agttctcctt cttccactaa ttcgccaaat gtgtttcgaa tgaaatacgt
     1081 gcgatcggac ctgtgtaaat tctggcgaaa gtaagcgagg atgtcaggct gaatcagttc
     1141 tgcctgctgg tagatccagc gatctttttc atcgatgaga atccctggcc tggttttcat
     1201 tttcgctcgc attgaaacaa tgtcacttcc actgtttcta tttctgcctg aacttcccct
     1261 gtaactccgc cgtgcttgtt ctgactgtct attgcattgg ttctaaattg ggtctggtgg
     1321 ttgatctgct ttttagacac atatccacaa ttggctcccc gcgcagcggc ttccagggcc
     1381 acgtctctct ggaattcctc tgagcctgga tttccgtaaa aattgcgaaa gatgagacgt
     1441 ccgagcgctt ccactttcac gtccggctta cgggcataca gatggaatga ctcaggatta
     1501 tctttgcgcc tgtttgcata ttcagcatcc ggaatgaatt caaccgatct gcaggacgcg
     1561 agcgcaaata ttgcggcgaa tagtatttta cgccgcacgc caggcccgca gaatgctcca
     1621 tggccaaaaa agggtcgaaa gaaagttctc aaggcatcat cacctatatc agaacactgg
     1681 ctccacttgc atatgacgta tttactgcgt ggagagatcg tcccaaatgg gaacaaatta
     1741 tggccgatct agagcttgtg gcccaggaaa acgagaagtt gcgcaaacga tccgttctgt
     1801 taacagtgtt ccttggcgca cttttccttt ggaatgcggt gttgacatac tttcttttcg
     1861 cgcgctgaaa gagcattgct ttctgaattt gttgaccctt gtggataggc cgccataagg
     1921 gaatatgctc gatgattcgc agcaggatga ggccagtaaa gaggaaatcc gaaaaaaaga
     1981 gaatcctctg cgcattggcg tttgcagcct ggacgaactc gaagagaagg tgaaagcctt
     2041 ccgaatcatg aatcagacgg cactcaagaa acggtatgtt ctctctcgag aggacatcat
     2101 cacaagtgcc ggtgaaacga ttgttcaaaa gcggtcggag gtagatattt cccgggcaaa
     2161 gctgatgcgc cgccattttc agggagacaa gatcctcaaa acattccaac cggatgaagg
     2221 cattgtaatc atcagtgaca tgtccacagc agagggcatt cctctttcca tggatcttgt
     2281 gacgcaggtg atgaatctgg gcggaggtgc ctacgaggca ttcattgacc gcgttgattt
     2341 cttttctgat tttctgaatt tgctcaagaa agcattgttt ccaagacttg tcatcattgg
     2401 gtatctacca ggagacaggc tggatgtgga acagatgaac tttctgcgtg cacgcaagat
     2461 tgatcaatac atgcgcgtca ttgaactcac tcacaccgcg tttaagccaa agccttactt
     2521 tacgaaagtg aagcacatcg tgatcgatat ctcagagcca aagacatggg gacgattcat
     2581 cattgagctg gttcgcgaat acacgaagcc ctatttgttt gaagagtttt agtgttcctc
     2641 aatctacttt tctttcttct tctgtccccg gatcgcgcgc gcgaaatgta tcgcacatct
     2701 ctgaagctgg atgatgtgcg cgctgttgaa ctcctggccc gggcgtacga agaagataag
     2761 acaaatcaag atgtaaccta ccggctcggc tatctctacc ataagatgaa ccgtacccgt
     2821 gaagccgtgg actactattc aaaaactgtg cggcttaatt cctgccacga aagagcgctc
     2881 aacaatctgg gtaccattgc ctatcaggag aagaaaaagc aagaagcacg cagctattat
     2941 gagaaagcag cgaaatgcgg caaggatgca gttacaccac tctacaatct tgcaaatctg
     3001 gaaagtgagg aaggcaacga agaggccgcc cggtctctgt atgagagagt ggtccgcaag
     3061 gacccgaacc atgcccgaag tcggcacaat ctgggacttt tgtattttag aagatacaac
     3121 cagactggat cagacgtgga tctggaggcg gccgagaagc aactgaaaga ggcggtgcgg
     3181 ctatctccgt ccgaccctct cactttattt aattttgccc tggttctaga agccgggcaa
     3241 aagaccgatg aagcgatccg ggtgttgaag aaggcggatt ctttgtcttc cggcaagacc
     3301 atccagccgc gcatccgcga agccctggcg cggctcaaag ggaagggcta gcaaaactta
     3361 gacgcggtag aaatcgaggt tcttctgcag cacgtctggc agaggctctg aatacttgcg
     3421 atacttcatt tcgaattcgc gcaacttctt ggtgcggata tttacaaaac gattgtgaag
     3481 ttctgcgaat gcaggctgcg tggccacttt gtcgcgcaca ccttttgcga aaggaacgtg
     3541 tctatagaag atggctctca ccacgcgatt catcttcata ttgccttccg cttcgccctt
     3601 gagccagttg gaatagtcat ttgcgaagcg atcgcggtca cttctgaatc gtttgaattc
     3661 tgatgccagt ttctctttga tttccggaga tagctctctg ttctttttgt agaattgaac
     3721 gtagtccgta taatccgcag taatcgaagg tacggcgacg ttgttccatt cgaatccctg
     3781 aatgcttttt gtgagttccc agcggaaggc ggccattgca tctaccatga tagaaaacaa
     3841 atcggcagtg gcgaaaaccg gaatggcgat gcgccctttg gacgttctct tgttggggct
     3901 cgcgagatcc tgccacatca tcaccttggt tccaatagaa ggaacaatga tgaaataagg
     3961 aatgacttga agctggatga attctttgag gatgcctctg gactcgtcat tgaaaagggt
     4021 ctcccggtga aacagcgagt aatcaatggc cagaattttg tctatgcatt cggaaagctt
     4081 gtctgcggtc acaaatgctt tgtccagggg agtgttgatc tggtacctgg tcagaatagg
     4141 gaaggacgtt gcgggagaac cagacgtcag gcgaacattg atttccagaa agttcaggat
     4201 ttcatatcgc acgcgaatcg catatcctgc gtattcgtcc ggtacatccg tttctttttt
     4261 ccagcctttg tctttgtgtt cttgcttgag cttctcgaag aaacccattc ccatttcgtc
     4321 cagggacgga ggttctttct tatctcccac gtaagggagc cagtcgagtg cggatagtac
     4381 gctatatcct ggttttccgc gcgtggagtc tttgcactga gtgagctgtg caatgtgctc
     4441 atcatcgagc atttcttcat caaagaaacc aaagcgaagc atccagtcta cgggaagcgg
     4501 aaggatcttt gtttccttca tcttgaagaa cgtcttttcg tatatgctcc agtaggtctt
     4561 gttgatggct ctacgtactt tgcgtggatc tccacccgaa tcgagcgggt tattgaagga
     4621 cttcagagtt tttagatctg cgagtagctt gcgcccgtct tctgccggga gctgaacgaa
     4681 gttgagtatc ttgccaatgc ttcctgcgag ttctttcttg agcgcctgga tgtccactcc
     4741 tgcggcaacg gcagcggcag ttgcttcctg cgctttctct tctggtttct gtgtcttcac
     4801 catttctctg agattgtcca gagaagcggc gatcgatcca attggttttc caaacatgct
     4861 ttcgtgtctg gacttcaact tttctgcgaa cgtgaccagg aatttgacaa cgccgattac
     4921 gttgggtcgc ccttcttctc cctgaacatc tgcaaccagg aagaactttt caataaaact
     4981 gtattcgcca gataacactg cgtttgtgct ttctagaatg gtctcttcta tctggcgaag
     5041 ttctccgatt ccagcataca cgatggtggc aagcttcgca gagataattt gcaggagggc
     5101 cgggtctgct ttgaacatgt ttccctgcag gtccgccggt agactcagga ttcgccgaag
     5161 aaaagtgaac tcttcttcat caaactccga ttccatttca taatgtttct taagtaaact
     5221 cgaatgatcc tgttcgagcc agcccgcggt gatgatgtca ggaatctctc cgccgctgga
     5281 ctggaactct tcgagaagca ccctggtttg cggcacgaca ggatcgagga cctggcctcc
     5341 gccgcctgga gcctgttgga atgcacccgg atttaatttg tagtatgcga gggaaagatt
     5401 gtcgattgcc ttctgaattt ctccgccaaa ttcggaagcc gcgacgaatg ttttgcgaga
     5461 tagaattgct tcttgcacca tggatcgagc agctgcgatt ccaacgttga gtttgcccag
     5521 gaccagggag ttgtagttgc ccgcaggatt gaccggaaag gcgctgatga ctgtatcttt
     5581 ggatgcggtg tagctggccg cgtaaggctt ttgtagcatc agggctgaaa atccgggcac
     5641 actgcttggc ccgaggttgt agagctttcg tccagatggt tcgcggagtg taacttcgcc
     5701 ttcatgaacc atccagagca tggtggccgc tttgccggct tcgaacaaga ctgttccggc
     5761 agggatcttt gcctgctgta gagcttttgc tgggtccagg gccattgaat tgtcttccta
     5821 ctattttgaa gctcgtcgtg ccactcaaaa actaacgtga tccaaaaatg agactgccga
     5881 tccgaagcag ggtcgaccct tcctcgaggg caatctcata gtcctgggac attcccatgg
     5941 acagttccat tccgctgaat cgctcatcgc ggatgcggcg cagggatcta aatacttctc
     6001 tggtcttttt tggctcagag tcgctcggcc ccatggtcat gagacctgcg aattcaaatc
     6061 caggtccagc gggcggttcg cccagggagt ccagttcttc aatcgtcatt ccgcccatct
     6121 tgctctgttc gccggtcagg tttagctgaa tgaagtatct aaggctttcg cttcttccat
     6181 ggcatgcttt gtttaacgcc tggattccgg agagcgaaga ggcgccgtgc acaacctgga
     6241 agaggccact gatctggcga gcattgccag attgcagggg gccgatgtga tgatagatgg
     6301 gcatttgttc aggtgcaaga cctgttgtgt ccacggccgg gattttgtcg cgtgcctctg
     6361 cgaatctgtt ttctccaaag tcgcgatgcc ctgcgcgaag aagctccgta attttttcgg
     6421 cgggatgagt cttgctgatg gcgaccactc gaacttctgg gtgccccagt tgttcgagcc
     6481 tcgctttcag tctttcaaga ttctgaaggg acactgcgag tgagcctggc cactctgtct
     6541 ggatcgatgg gacgcatgga cgtgtcgtgt gcgagtttag gatcgatctt gtattcttcc
     6601 ggcttgaatc cgagcgccac catgttctcc atttccactt gcagagactt gagttgtttt
     6661 cccagaacat ccagtctgtc cgtgatcttg tcgcgcagat cgcctgtatc cttttccggg
     6721 aagagtggag caccatcaaa ctggagattc tctcttcctt ctaccgcgtc cacggaagca
     6781 agacgagcct gttcgcggct gtaatcatgc tgcacgtgag ccaggtgtcc ctgaatctgc
     6841 atcagcctgg aatgcatgag tgctggctgg agttcccggc tcgtgtccgg tccggtcccc
     6901 tgagaggcga tccggtttga gcggcgattc agcagagttt ctgctgaact cagtaggtat
     6961 ttgccggaga tctgcatgat gatgttcctc taataatatt atgtcgcaca ggctgtttct
     7021 tgcgccgagt tccgtctact tagtttttcg atcagtggac ccggtctctg tagtggtttt
     7081 ttctctcgaa atatgtcctt ttatcgaatt tttctcgctc agactcagct tcctgtgtct
     7141 ctggacccat ggatcgttca cgcgctgctg tggccctggc actcggattg gccactgtat
     7201 ctttactcct ggggttgctt caaatcggca tggcccgcaa atcgcaggac ttcactgcgc
     7261 ccgcaggtgc gctcttcaac
//
LOCUS       DAORFF010000130         7209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5230_length_7209_cov_4.318004, whole genome shotgun sequence.
ACCESSION   DAORFF010000130 DAORFF010000000
VERSION     DAORFF010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7209
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5230_length_7209_cov_4.318004"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /gene="htpG"
                     /locus_tag="PKK76_06945"
     CDS             <1..1494
                     /gene="htpG"
                     /locus_tag="PKK76_06945"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNL68244.1"
                     /translation="GIIGHFGLGFYSAFMVAKRVEIDTRSYKPDAEAAYWTSEGGEEY
                     ESGPGSRTKRGTEIRLHMDDESKSHLDKLEVTELVRKYCDFLAVPVRVDGSQVNRKDA
                     LWSKQPNQVQKEEYVEFYKYLFPHQGDPLFHIHLNVDHPFRLQGILYFPRMLHELDVN
                     KNGVRIFCRNIFVSDEAQEILPKYLTMLQGVVDLPDLPLNVSRSYVQTDPEIRKIASH
                     ITKKVADRLNEEFTKNRTQYEQIWREIGPFVKYAMLSDEKFYDQAKASVLFEIAGQEN
                     KFCTIEEYQLQNKERAQEKIYYASDLIQQAGPMQMLKEQGIEAVYLPQVIDTHFIQFL
                     ESKGIGVHFTRVDAELAEHIVDAGASSKIVDPDAKSSEDRVRELFTKALANPKVTIRI
                     ESLKNDQVPALILLPEFMRRMSEMAAVYKMQDAKMGEEHTLVLNLKHPLIQALSRPGI
                     LAGEGPSKQERVARAIYGLARLSQGAVQPEEIGKFTGETYELLASVI"
     gene            1547..2668
                     /locus_tag="PKK76_06950"
     CDS             1547..2668
                     /locus_tag="PKK76_06950"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNL68245.1"
                     /translation="MRLWLSIFILIVSFSCKEAPRVRKPAKVTALVLFKVGSVRAGGR
                     EITTGEIIQDHELISVGAQSILDLQVRETEASIAMRLRENTEVTLAGFVDPEGQLNIS
                     PEQSKGTLLTQVEKLGAKERMLVRMPSALASVRGTKFETAIDPGNMARVTVLDGKVAA
                     RTRITELEDSAAASQIPELAQLTGALDKTETIIESGKSATLDPKAKDQWLEDSGLGPL
                     LNSPEFKALAGKETVSPEQIASAQAASAKHFQSPESQTKLKSSLSRPPAPPTVESVPK
                     EEWDRKLKEYEELISIEQAKAKDQTAAGPAVKARNDAIQAQLLSRIEQIMGKASETLL
                     LKNGTRIRGVIYQNGADYLVLTAEGSKTFSDAEVSGIEF"
     gene            2665..4827
                     /locus_tag="PKK76_06955"
     CDS             2665..4827
                     /locus_tag="PKK76_06955"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL68246.1"
                     /translation="MTNQSRITLEQSKRAMIRNVFLLLLLAAGCSAPLDRSGFPIDLT
                     QSRWRACSGFQSDFALGLPANANCIPVKSFPILLDRILPVPTGSGTHDWTLETHFSLE
                     SPGKRSVALYLPVIGENWEIFLNGVSVRREIHSSARGEIQTFRTIKGVHVDLPPALLR
                     EDNTLVFHIRGTAAVTSLYLNDYSGFYMRNGYEIDTAENLRAKNNELFSFMLYSVYFF
                     FGLYHIILYLSNRQQRYNLSFGLLSVMLAFYSILISNSVFSIFPTMDTAILKKAELVS
                     LFCLMPGISYFIVDYFEPGRFKWALRPALVWNISLALVAGFLPFQYARVALPIWQLTA
                     LVQLPFLLFLMIRYVIARDPDAYVMGATFLLALLSAVFDIVASFLVVSDVRLFQATFF
                     LFILSIVGTIALRLRRLEKSERALNDELRELSLAFYRFVPTQVLEQMEKSSAADLKVG
                     DNALRLMNVLFSDIRGFTTLSETMNPDDNFRFLNSYLARMEPLIAAESGYVDKFLGDG
                     IMALFSQAAGEVQSKTPSERGLDAAIAMRRTMPDYNNRRKNSGYPPIDIGIGLHTGPL
                     IIGAVGSSNRIDTTVIGNTVNVASRIEGMTSYYKAGIIISEALRTDLSSPDKYRIREI
                     GSILVKGKTHPISLYEVFDADPEEIAEFKSRTSAFISVGIAHFREKEFAEARKLFREV
                     LLEYPADSVAKVYYKLCTKFLSGPVPENWTGAIEVLRK"
     gene            4904..5245
                     /locus_tag="PKK76_06960"
     CDS             4904..5245
                     /locus_tag="PKK76_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68247.1"
                     /translation="MKKTLLISVTLTLSALLLVNANCKKKGPDLSKYDEICATVTKCD
                     QNPQSKMLLMQPEACQKMFAGLDKSPQTAAFTPQVIDCIQKAPCAKLDTMKCFAASAQ
                     GIMPMMPAPNK"
     gene            complement(5292..>7209)
                     /locus_tag="PKK76_06965"
     CDS             complement(5292..>7209)
                     /locus_tag="PKK76_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNL68248.1"
                     /translation="LGLSFLVARIVRRLGQPEVIGEMIAGIMLGPSLLGFLWPDAYTA
                     LFPPASLGNLASLSQLGLMIFLFLIGMEVDLSHIRNHLRAAIFISHAAIVIPFTLGVL
                     LALYIYRQYSPAGVDFLSFGLFIGISVSITAFPVLARIVQERGLLGSPTGRMALTVAA
                     IDDATAWCILALVVALVQAKGVASAFVTVVLALTYVLVMLFVVRPFLNRMGTVYYTRE
                     IIGRGVMASIILLILGSAVLTQLIGIHALFGAFMAGVVLPADSRFGHLVAEKLRDFAS
                     VILLPLFFALTGVRTDFVLIFKSGAWDVMGLVLLVAIGGKVGGGMLSALLVGMKPKPA
                     LELGVLINTRGLIELIILNVGYDLGILTRELFAVLVLMAFITTFMTGPILSIMHRKRS
                     EDVPVAVKPPSARGILFSFGPVLTGISLLRIASLLRKEGERITAAHFHRSSELLGRDP
                     VEGKIFRALHARAADSGLQLATVEGVATDISEDILSTIQKERPELLLLGGARSMFSKN
                     RIGGKVGEVLKDASSAVGIYVEKDLPEEFRQAGILFSRRKDGFLMDYAERLRGSGVDV
                     IILPTTEDLMEHLVAAQCPFPMRLDNDLYGEFDLLVVSVPHWLEKRYLYSDWMDDPPC
                     SLLLIRESSEPAGE"
BASE COUNT         1999 a         1868 c         1730 g         1612 t
ORIGIN      
        1 ggaatcatcg ggcacttcgg gcttggattc tactcagcat tcatggttgc aaaacgagtg
       61 gagatcgata ctcgcagtta caagccggac gcagaggcgg catactggac ttcggaagga
      121 ggcgaagagt acgaaagcgg gccagggtcg cgaacaaaac gtggaactga aattcgcctt
      181 cacatggacg atgaatccaa atcgcattta gacaaactgg aagttacaga actggtccga
      241 aaatattgcg actttctggc ggtgcctgta agagtagatg gctcccaggt gaaccgcaaa
      301 gacgcactct ggagcaagca gcccaatcaa gttcagaaag aagagtacgt tgaattttat
      361 aagtatttat ttcctcacca gggtgatcct ctctttcaca tccacctgaa cgtggaccat
      421 ccatttcggc tgcaaggaat tctatacttc ccgcggatgc tgcatgaact cgatgtaaac
      481 aagaacggcg ttcggatttt ttgtagaaac attttcgtat cggatgaggc gcaggaaatt
      541 cttccaaagt atctcaccat gctccagggc gttgtggatt tgccagacct tccactcaac
      601 gtgtccaggt cttatgtgca aacagatcca gaaattcgca agattgcctc tcacatcacg
      661 aagaaagttg cggacagact caacgaagaa ttcacgaaaa atagaactca gtatgagcag
      721 atctggagag aaattggtcc gtttgtaaaa tatgccatgc tcagcgatga gaaattctac
      781 gaccaggcaa aggcatctgt tctttttgaa atcgccggac aggaaaacaa attctgcacc
      841 atcgaagaat atcaattgca gaacaaagaa cgagctcagg aaaagatcta ctacgcaagc
      901 gatctcatcc agcaggccgg gcctatgcaa atgctgaaag agcagggcat tgaagcggtg
      961 tatctcccgc aggtgatcga cactcatttc attcaatttc tagaatcaaa aggaattggc
     1021 gtacacttca ctcgcgtgga tgcagagctc gccgaacaca tcgtagatgc aggcgcctct
     1081 tccaaaatcg tggatccgga cgcaaagagc agcgaagaca gagtgcgaga actattcaca
     1141 aaggcactgg ccaatcctaa agtaaccatc cgaatcgaat cgcttaaaaa cgatcaggtg
     1201 cctgctttga tcctgcttcc agagttcatg agacgaatgt ctgaaatggc agcggtgtac
     1261 aagatgcagg atgcgaagat gggagaagaa catactcttg ttttgaatct caagcatccc
     1321 ttgatccagg ctctctctcg cccaggaatt cttgcgggag aaggaccttc caaacaggag
     1381 agagtagctc gcgcgatcta cggactggcg cgcctatcgc agggagcggt tcaaccagaa
     1441 gaaatcggga aattcacggg agaaacctat gaacttcttg catctgtcat ctgagccctg
     1501 acgattttta gattgtaaac cagagtgttt gagaatagag tcccccatgc gtctgtggct
     1561 ttctattttc attctgatcg tgagcttctc ttgcaaggaa gcgcccagag tccgaaaacc
     1621 tgcgaaagtt actgcacttg ttcttttcaa ggtgggctcc gttcgggcag ggggccgcga
     1681 aatcacaacc ggggaaatca tccaggatca tgaactcatt tcagtgggag ctcagtccat
     1741 tctggatctc caggtgcgcg aaacagaagc atccatcgcc atgcgtctgc gagaaaatac
     1801 agaagtcact ctggctgggt ttgtggaccc ggaaggccag ctgaatattt ctcctgaaca
     1861 atcgaaaggc acactcctca ctcaggttga aaaactcggg gcaaaggaaa gaatgctcgt
     1921 gagaatgcct tccgctcttg catctgtaag gggcactaaa ttcgaaacag cgatcgatcc
     1981 aggaaatatg gcgcgcgtca ctgtactcga cggaaaagtc gcagcccgca ccagaatcac
     2041 agaactggaa gattcggccg ccgcgtcgca aatcccagaa ctagcacaac tgacgggagc
     2101 cctggacaaa acagaaacca tcatcgaaag cggcaagagc gccacgctcg atccaaaagc
     2161 aaaagaccag tggctagaag actcgggcct ggggccgctt ctcaattctc cggaattcaa
     2221 agctctcgcc ggaaaagaga ccgtttctcc ggagcaaatc gcaagcgcgc aggcggccag
     2281 cgcgaaacac tttcagagtc cagagtcaca aacaaaactg aaatcttccc tgagccgccc
     2341 gcctgcacct cccacagtag agtctgttcc aaaagaagaa tgggatcgca agctcaaaga
     2401 atacgaagaa cttatttcca ttgaacaggc aaaagcaaaa gaccagactg cggctggacc
     2461 cgcagtcaag gcaagaaacg acgcgataca ggcgcagctt ctctcgcgca ttgaacagat
     2521 catgggcaaa gcttcggaaa ccctgctgct taaaaatgga accagaattc gcggagtcat
     2581 ttaccagaac ggtgcggact atctcgtgct aactgcggaa ggaagcaaaa cattcagcga
     2641 tgcagaagtc agcggcattg aattttgacc aaccaatccc gcataacgtt agagcaatcc
     2701 aaaagggcca tgattcgaaa cgtattcctt ctactcctgc ttgccgcagg ctgttcagcg
     2761 cctttggacc ggtcaggatt cccaatcgat ctgacgcagt caagatggcg cgcctgcagc
     2821 ggatttcaat cggacttcgc cctgggcctt ccggcgaatg cgaactgcat ccccgtaaaa
     2881 tcatttccaa tcttactgga ccgcattctt cccgttccaa caggttctgg gactcatgac
     2941 tggactctcg aaactcactt ctcactcgaa tcacccggca agagaagcgt cgcactgtat
     3001 ttgcctgtga tcggcgaaaa ctgggaaata tttttaaacg gcgtatctgt tcgccgcgaa
     3061 atccattcat ctgctcgagg agaaatccag acatttcgca caatcaaagg agtacatgta
     3121 gatcttccgc ctgcattgct acgcgaagac aacacacttg tctttcacat caggggaact
     3181 gcagccgtca cttctcttta cctgaacgat tattcaggat tctacatgcg aaacggttac
     3241 gaaatcgaca cagcggaaaa cctgcgtgcg aagaacaacg aacttttctc cttcatgctc
     3301 tattctgttt attttttctt tggtctatat cacatcattc tgtacttgag caatcgccag
     3361 caaagatata acctttcctt cggtctgctc tccgtgatgc tcgcattcta cagtatcttg
     3421 atctcaaatt cagtcttttc cattttccca accatggaca cggcgattct aaagaaggcc
     3481 gaacttgttt ctctgttctg tctgatgcct gggatcagct atttcattgt agactatttt
     3541 gaacctgggc gattcaaatg ggcactccgg ccagctctag tgtggaatat ctctctggca
     3601 cttgtggccg ggtttcttcc atttcaatac gcaagagtgg cactcccgat ctggcaattg
     3661 acagcgctcg tacagcttcc gttccttttg tttttgatga tccgttacgt gatcgcacgt
     3721 gatccagacg catacgtaat gggggccact tttcttctcg ccctcttgtc tgcagtcttt
     3781 gacattgttg cgtcttttct cgtggtctct gacgttcgtc tgtttcaagc cacgttcttt
     3841 ctgttcatcc tatcgattgt tggaaccatc gcactcaggc tcaggcgtct ggagaaatcc
     3901 gaaagagcac tcaatgatga gcttcgcgag ctttcccttg cgttttatag atttgtgccg
     3961 acgcaggtgc tcgaacaaat ggagaaatca tctgccgcag acctcaaggt cggggacaac
     4021 gctcttcggc tcatgaacgt gctgttctct gacattcgcg gattcacgac tctgtccgaa
     4081 accatgaatc cggatgacaa cttccgcttc ttgaattcgt acctggcgcg aatggaacct
     4141 ctgattgcgg cagaaagtgg ctatgtagac aaattcctgg gcgatggcat catggctttg
     4201 ttctcacagg ctgcaggaga agttcaatcc aagactccat ctgagcgcgg cctcgacgcg
     4261 gccattgcca tgcgccgcac gatgcctgat tacaacaatc gcagaaaaaa ctcgggctac
     4321 ccgcctattg acattggaat cggtctgcac actggtccac tgatcattgg agcggtaggc
     4381 agctccaatc gaatcgatac tactgtaatc ggaaacacgg tcaacgttgc ctctcgtatt
     4441 gaagggatga cgtcttacta caaggcgggc atcatcattt cagaggcact tcgcacggat
     4501 ctaagctcgc ctgacaaata tcgaatcaga gaaatcggct ccattctggt gaaaggcaaa
     4561 acgcatccga tctctctata tgaagtcttt gacgcggacc cggaagaaat tgctgaattc
     4621 aagtccagaa ccagcgcatt catttctgtt ggaatcgctc atttcagaga aaaagaattt
     4681 gcggaagcaa gaaagctctt tcgcgaggtg ctcctcgagt atccggctga ctctgtggcg
     4741 aaagtgtact acaaactctg caccaaattc ctgtcgggac ctgtgccaga aaactggacg
     4801 ggcgccatcg aggttctgcg aaagtgatgt tgcggcattc gggtggcgaa aacagcacgc
     4861 tcgaatgggt tgacaatgtg gcattttgtt gcaaaattgc cgcatgaaaa aaacacttct
     4921 gatcagtgtg actctgacgc tatccgcctt gcttttggta aatgcaaact gcaagaagaa
     4981 aggccccgat ctctccaaat acgatgagat ttgcgccact gtaaccaaat gcgaccagaa
     5041 tccgcaatcg aagatgcttc tgatgcagcc tgaagcctgc cagaagatgt tcgcaggcct
     5101 ggacaagagc ccacagacgg ctgcttttac acctcaagtg atcgattgca tccaaaaagc
     5161 gccatgtgct aaactggata caatgaaatg cttcgcagca tctgctcagg gtatcatgcc
     5221 aatgatgcca gctccaaaca aataatacgc ttcactcata agaaaactca aaacccgctt
     5281 cggcgggttt tttattcccc ggctggctct gagctttcgc gaatcaacaa aagagagcag
     5341 ggcggatcgt ccatccagtc cgaatacaga tacctttttt ccagccagtg cggcacgctg
     5401 accaccagca aatcgaattc accgtaaaga tcgttgtcca aacgcatcgg gaagggacac
     5461 tgcgcggcca ccaggtgttc catcaaatct tccgtagtcg gaaggataat cacatcgact
     5521 ccggatccac gaagccgctc cgcgtagtcc atgagaaagc cgtcttttct gcgtgagaac
     5581 aaaatccctg cctgcctgaa ttcttccggg agatctttct caacgtaaat tccaactgca
     5641 ctcgaggcgt ctttgagaac ctctcccact ttgccgccaa tcctgttctt gctgaacatg
     5701 gagcgagctc ctcccaggag caaaagttcc ggccgctcct tctggatggt ggaaagtatg
     5761 tcttcggaaa tatccgtggc cacaccttcc acagtggcca gctgcaatcc ggaatccgct
     5821 gctcttgcat gcaatgctcg gaagatcttt ccctctacag gatcgcgtcc taatagttca
     5881 gaacttctat gaaaatgagc ggccgttatg cgctcaccct cctttcgcag aaggcttgct
     5941 atgcgaagaa gggaaatgcc cgtcaagaca ggaccaaagg aaaatagaat tccgcgcgca
     6001 gagggtggct taactgcaac aggcacgtcc tcggatcgct tgcgatgcat aatgctgagg
     6061 ataggaccag tcatgaaggt agtgatgaac gccattaata ccaggactgc gaaaagctct
     6121 ctggtcagaa ttccgaggtc atagccgacg ttcaggatga tcagttcgat gagaccgcga
     6181 gtgttgatca atacaccgag ctcaagcgca ggctttggct tcattccgac gagcaatgca
     6241 gaaagcattc cgcctcccac ttttccgcca atcgccacca gaagtacaag gcccattacg
     6301 tcccaggcgc cagatttaaa aatcaaaacg aaatcagtgc gaacaccagt cagtgcgaaa
     6361 aacaaaggaa gtagaatgac gcttgcgaaa tcgcgcagtt tctctgccac cagatgacca
     6421 aacctggaat cggcaggaag cacaacgccc gccataaatg caccaaaaag cgcatgaatg
     6481 ccaatcaatt gagtcaaaac agcagaaccc agaatcaata gaatgatgga tgccattact
     6541 ccacgcccaa tgatttcgcg cgtatagtag acggttccca ttcgattcag gaatggcctg
     6601 acaacaaaca acatcacgag cacgtaggta agagccagca ctacagttac aaaagcagat
     6661 gcgactcctt tcgcctggac cagcgcaacc accagcgcga gaatgcacca ggcagtcgcg
     6721 tcgtcgatgg ctgccactgt aagagccatt ctgccggtag gcgaaccaag aaggccccgt
     6781 tcttgaacaa tgcgcgccag aaccgggaaa gcagtgatgc tcacggaaat ccctatgaac
     6841 aaaccgaaag ataggaaatc cacacctgct ggcgaatact gtctatagat gtaaagagcg
     6901 agtaacacac ctaaggtgaa ggggataaca atggccgcat gactgatgaa aatagcggcg
     6961 cgaagatgat tgcgaatgtg actcaaatcg acttccattc cgatgagaaa aaggaagatc
     7021 atcaaaccga gttgagatag actggccagg ttcccaagag atgccggagg aaagagggcc
     7081 gtgtaagcat ccggccacag aaatcccaga agagacggac caagcataat gccagcgatc
     7141 atttcaccga tcacttcagg ctggcccaga cgtctgacga tgcgtgcaac cagaaaggaa
     7201 agtccgaga
//
LOCUS       DAORFF010000131         7180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5258_length_7180_cov_3.329404, whole genome shotgun sequence.
ACCESSION   DAORFF010000131 DAORFF010000000
VERSION     DAORFF010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7180
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5258_length_7180_cov_3.329404"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="PKK76_06970"
     CDS             <1..1024
                     /locus_tag="PKK76_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNL68249.1"
                     /translation="RKLHHGLITPQRTNEMKKAIVLAIVLLSACKQGAWVSVDGSRLT
                     ESDVEKEMPDQYKRLKEEYNSQMRDLLEQVAVKKMMQKAAKEKGVDEETYARQISEKA
                     PEPTEKQIADFYEQIKKTGQTDGQSLAQLHSRIAMHLKQEGGREAMQNEISELKKKYN
                     YTYDFSRTKVETAGNPERGNASAKVTVVEFSDFECPYCIRVQPTLQKLREKYNGKIRW
                     VFKDFPLNFHPHAMDMHIGSKCVYRVNKDHYWEFFDKMFAQDRSADILTKEGTAKLAA
                     SLGTDMNKYKACTEDPAVAAEIKDSIKQGEAVGVSGTPAFFINGRMLSGAQPMAAFEE
                     VIQQEL"
     gene            1115..1390
                     /locus_tag="PKK76_06975"
     CDS             1115..1390
                     /locus_tag="PKK76_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68250.1"
                     /translation="MVSESGLGSHSNTRIVFRLAALGFFNIVIILESNPQDSDVPKYR
                     ASLIAVSAWLAGRVEFRAACAAIQPPKWRAAQMISLAAVFVKEKSRE"
     gene            complement(1431..1802)
                     /locus_tag="PKK76_06980"
     CDS             complement(1431..1802)
                     /locus_tag="PKK76_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HNL68251.1"
                     /translation="MEEQQMQQAAAGGMAGNLIGLVIAVIYIVGLYKTFEKAGQPGWA
                     AIIPLYNMIVLLKVAGKPMWWILLLLCTGPIGMILVGMAVAERFGKSSVFGIVALGLF
                     GFVGYPMLGFSDATYTAPPAA"
     gene            2061..3554
                     /gene="glpK"
                     /locus_tag="PKK76_06985"
     CDS             2061..3554
                     /gene="glpK"
                     /locus_tag="PKK76_06985"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNL68252.1"
                     /translation="MSKPFLIAIDQGTTGSRVFCFDTAGKVLSTAYREFTQHFPKPGW
                     VEHDAMEIWTGVCSLLAEALSKGGLDAKNAAGIGITNQRETCVVWDRKTGEPVHRAIV
                     WQCRRTADFCEELKKRGLADEVRKKTGLVIDAYFSGTKIRWFLENVAGVRARAEKGEL
                     MAGTIDTWLLYRLTGEWKTDYTNASRTLLYNINKKKWDEDLCKTLNVPIGLLPEVQAS
                     RSRFGTTKKVPGLPDGLPVLSIAGDQQAALFGQLCVKPGQAKNTYGTGCFLLFQTGQK
                     FIISKSGLLTTLAVDEKGGVSYALEGSVFIGGAVIQWLRDFMKFFASARETEDIIKNI
                     KDDEDDVVFVPAFAGLGAPHWDMQARGAVFGLTRDTDPARMTRAALKSIALQSHDLVR
                     AMEKDTGEKLPSLRVDGGATANHYLMQFQSDILNCPVERPQNADTTALGVAYLAGMEA
                     GIWKNVDDLLKLQEDRTIFRPAMHAERRERELRYWNKGVERVKNWSE"
     gene            complement(3535..5193)
                     /locus_tag="PKK76_06990"
     CDS             complement(3535..5193)
                     /locus_tag="PKK76_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL68253.1"
                     /translation="MSKVKNTFRQERRRLEDFKRSMIELRATRDVCRAVLEFLTEALD
                     SEKAGIYIWKEEEGTFSVWPDQPGVEPRFSLMDPAIAYFGEHPQVRHVETISSDEDLT
                     DDARELLHRFFTLTNSRVLIPLALNESLVGVIFLAPPPSVKLGRRETALIEEVRSLGV
                     MSLSNSILYARLEGILGHLEEKVRERTQELESTQSQLVQSEKMASLGVMVAGIAHEIN
                     TPASVIQAAAENVEKNISYLLTNLEMLRRVEAKTEESAGSGAADRILHAIESLLKRPD
                     AGPIKDLFLRKRDMTRRMEERAIDHARELSALFAENGLDVDDSLLGDLAAIFQAGKDS
                     GAFLTFLSEAANAARNLKNLKTSIRAIARIVKALKSYSHLDSGAFQLADVHESLDNTI
                     VLLGSVLKTSLHIEKRYAELPDILCNSDELAQVWTNLIQNSAQAMKGNADSRVVVTTS
                     VADLWGRPAVQIQIADNGPGIPEEVLPRIWDPFFTTKDQGEGTGLGLSIVRNIVHKHQ
                     GIIRVESSSGGAVFQIILPVEPPAGFDTGSTIYKFAHKPTQTSS"
     gene            5221..6276
                     /gene="rsgA"
                     /locus_tag="PKK76_06995"
     CDS             5221..6276
                     /gene="rsgA"
                     /locus_tag="PKK76_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNL68254.1"
                     /translation="MSAESGSFEAVVSSVFGAYYDMLVLPDCLETRRGRLRGRLRLAG
                     RKNEESWDHIPERHLILVGDRVHCVQENDESLIESRRERKNVLFRSSRFEQQGLGANI
                     DRAVVVMSFAYPDVSQGFIDRFLASCHAGGVEPVILFTKSDLTDDELREGCLELYRGL
                     GYSAFAVNLLDPKGEDWESICSLLSNGISLFSGRSGTGKSTLLNRLLGNENMATGAVS
                     ESTKKGRHTTTNSTMVRDPKTGALYIDTPGVKEWGVMNLDRKDIFESFPEIRAAATLC
                     KFRDCLHETGAEGCAVAEKLEKPGEFHPGRILSLKSMLDSLETGERIRKGDYIKATGR
                     MRQGSKYALKNRPPEHR"
     gene            6284..>7180
                     /locus_tag="PKK76_07000"
     CDS             6284..>7180
                     /locus_tag="PKK76_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68255.1"
                     /translation="MRSWLALAFFCLSHAVYAECDREWVPGNSLDLSGSWKYAQAGEA
                     SSPELDDSGWKRTSVPGLWGKNPALANYRGELWMRCHVTLNGEKPENVALNLGLVEDM
                     DEAYFNGNLVGRTGSFARNTVDIETERLYALPAALWREGDNVIAVRIRGSSTESGIHD
                     QVRIENEQLAARRNFLKNVPAIVCSVLYILTSGFFLLFYVFFRHRYEHMFFALFSAFL
                     GFYHLIRTNLRLDIFTSFEFSYQVELALLFCLPGLFINYLIYLLKLKRPPIVWALMGF
                     SLILVIGALLSGAGKWPIKQAWQ"
BASE COUNT         1904 a         1839 c         1811 g         1626 t
ORIGIN      
        1 ccggaagctg catcatggat tgatcacacc tcagaggacc aatgaaatga aaaaagcaat
       61 tgtgctcgcg atcgtactgt tgtccgcgtg taaacagggt gcctgggttt ccgtagatgg
      121 aagcagactc actgaatcag acgttgaaaa agaaatgcct gatcaataca agagactgaa
      181 agaagaatac aactctcaga tgcgtgatct gctggagcag gttgcagtca aaaagatgat
      241 gcagaaggct gcaaaagaaa agggagtaga tgaagaaacc tacgctcgcc agatttcaga
      301 aaaagctcca gagccaacag aaaaacaaat cgctgatttc tacgaacaaa tcaagaaaac
      361 aggacaaact gacggccaga gcctggcgca gcttcacagc agaatcgcaa tgcacctcaa
      421 gcaagaaggt gggcgcgaag caatgcagaa cgaaatctct gagctaaaaa agaagtataa
      481 ctatacatac gatttctcac gcaccaaagt ggaaaccgcc ggaaatcctg aacgcggcaa
      541 tgcttctgcg aaagtcactg tggttgagtt ctctgatttc gaatgtcctt attgcattcg
      601 cgttcagcct accctccaga aactgcgcga aaaatacaat ggcaaaatcc gctgggtgtt
      661 taaagatttc cctctgaact tccatcctca tgcgatggac atgcacattg gttccaaatg
      721 cgtgtatcgc gtgaacaaag accattactg ggaattcttt gacaagatgt tcgctcagga
      781 ccgctctgcg gacattctca caaaagaagg aacagcaaag ctcgcggctt ctcttggaac
      841 agacatgaac aaatacaaag cctgcacaga agatcctgca gtggccgctg aaatcaaaga
      901 cagcatcaag caaggagaag cagtgggagt atctggaacg ccagcgttct tcatcaatgg
      961 tagaatgctt tccggagccc agcctatggc tgcatttgaa gaagtgatcc agcaggaact
     1021 ttaagaactg ccgggacaac gaaaagcgac ggaaaaaagc agcaggtact tcgtcgcata
     1081 acgttacgga atcgatcttt cccgcacgga aggaatggtt tcggaatctg gtttaggctc
     1141 tcatagtaat acgcgcatag tctttcgcct tgctgcgctg ggcttcttca atatcgtaat
     1201 cattctggag tccaatccac aagactccga cgttccaaag tatcgagcaa gtctgattgc
     1261 tgtgtctgcc tggctcgccg gccgcgtaga atttcgcgca gcctgcgcgg caatccaacc
     1321 gccaaagtgg agggcggcgc agatgatcag cctggcggcg gtctttgtta aggagaaatc
     1381 aagagaatag aaaatgcgat cccgcgccgt gggcgcggga tgcttgagag ttacgctgct
     1441 ggtggagcag tgtaagttgc gtcgctgaat ccaagcattg gataaccgac aaaaccgaac
     1501 agacccaggg caacaatgcc gaagactgaa gatttgccga agcgttctgc tacagccata
     1561 ccaaccagaa tcattccaat tgggcccgtg cagaggagaa gcaggatcca ccacattggc
     1621 tttccagcta ctttcagaag cacgatcatg ttgtagagag ggataatcgc tgcccagcct
     1681 ggctgacctg ctttttcgaa ggtcttgtac aggccaacaa tatagattac cgcgataaca
     1741 agtccaatca agttaccagc cattcccccg gcggcagctt gctgcatttg ttgttcttcc
     1801 atttttgaac actcctatgt tttttcacgg ctttcgcacg cgaatctgcc agtaacctga
     1861 gaatcaccgc gaagaaggtc aaacgaaaat tttggatcac tcacgatttg gccacgtttc
     1921 ctttggatct tttgcctgtc tggagctcga gctgtcccgg acgccggagc ctgcctgaaa
     1981 agaaagcaca ggctgttgcc gaaaagcaca tcccggcatt ttcggcttgc acatcttcct
     2041 atcaccaggg cgaatcgcac atgtccaaac catttctcat tgccatcgac cagggaacta
     2101 caggctccag agtattctgc tttgatacag cgggcaaagt tctctctact gcttaccgtg
     2161 aattcaccca gcacttcccg aaaccagggt gggtcgaaca tgatgccatg gaaatctgga
     2221 cgggagtgtg ctctcttctg gcagaagctc tgagcaaagg cggactggac gcaaagaatg
     2281 cagcaggcat tggcattaca aaccagaggg agacctgcgt ggtgtgggac cgcaaaactg
     2341 gagagccagt tcatcgtgcc attgtgtggc aatgcagacg cacggctgac ttctgcgaag
     2401 aactcaaaaa gcgcggactc gcggatgagg ttcgtaagaa aacaggactg gtcatcgatg
     2461 catatttttc cggcacaaag attcgctggt tcctggaaaa cgtagcagga gtgcgcgctc
     2521 gcgctgagaa aggcgaactg atggcaggca ccatagacac gtggttgctc tatcgcctca
     2581 ctggagaatg gaaaacagat tatacaaatg cttcgcgcac tcttctgtac aatataaata
     2641 agaagaaatg ggacgaagat ctctgcaaaa cactaaacgt gccaatcggt ctcttgcctg
     2701 aagtgcaggc aagccgcagc agattcggaa ccacaaagaa agttccagga cttccagacg
     2761 gccttccagt actatcgatt gcaggagacc agcaggccgc tctctttgga cagctctgcg
     2821 tgaagccagg acaggcaaag aatacatacg gcacaggctg cttcctccta ttccagacag
     2881 gacagaaatt catcatcagc aaatcagggc tgttgactac acttgcagta gatgaaaaag
     2941 gcggagtttc gtatgcactc gaaggatctg tgttcattgg tggcgctgta atccaatggc
     3001 tgcgcgattt catgaagttc ttcgccagcg cacgcgaaac agaagacatc atcaagaaca
     3061 tcaaagacga tgaagacgat gttgtttttg ttccggcatt cgcagggctc ggagctccgc
     3121 actgggacat gcaggctcgc ggtgctgtat ttggtctaac ccgcgacacg gatccggcgc
     3181 gcatgactcg cgctgcactc aagtccattg cgctgcaatc gcacgatctg gtgcgagcca
     3241 tggaaaaaga cacaggagaa aaactccctt cccttcgcgt agacggaggc gccactgcga
     3301 accactacct catgcagttc caatctgaca tactcaattg ccctgtagag cgtccacaaa
     3361 acgcagatac aaccgctctg ggtgtagcct accttgcggg aatggaagca ggcatctgga
     3421 agaacgtaga cgatctgctg aaactgcagg aagatagaac tatcttcaga ccggccatgc
     3481 atgcggagag acgcgaaaga gagcttcgct actggaataa aggcgtggaa agagttaaga
     3541 actggtctga gtaggcttgt gtgcaaactt gtagatcgtg cttccggtgt caaaaccggc
     3601 aggcggttct acaggcaata tgatctggaa aacagctccg cccgaagaag attctacccg
     3661 gatgattccc tggtgcttat gcacgatgtt tcgcacaatc gatagcccga ggcctgtacc
     3721 ttctccctga tctttcgtgg tgaaaaaagg atcccagata cgaggaagta cttcctctgg
     3781 aattccaggt ccattgtctg caatctgaat ttgaactgcg ggcctacccc aaagatccgc
     3841 cacagatgta gtcacaacca cgcgagagtc cgcatttcct ttcattgcct gcgctgaatt
     3901 ctgaatgagg ttggtccaca cctgtgctag ctcgtcactg ttgcacaaaa tgtccggcaa
     3961 ttccgcataa cgtttctcaa tatgcaaaga agtcttgagc acacttccca gaagcacaat
     4021 ggtattatcc agactttcat gaacgtctgc gagctggaat gctccagaat caagatgaga
     4081 ataagacttg agtgctttta caatgcgcgc aatcgcacgt atggaagttt tcagattctt
     4141 caagttgcgc gcagcgttgg ccgcttcaga aagaaacgtc aggaacgctc cagaatcttt
     4201 tcctgcctga aagatggcag ccagatcacc caaaagagag tcgtccacgt ccagtccgtt
     4261 ctcagcaaat aaagcgctga gctcacgtgc gtggtcaatg gctcgttcct ccattcttct
     4321 ggtcatgtcg cgctttcgca aaaacagatc tttgattggg cctgcgtccg gccttttgag
     4381 aagggattcg atggcgtgaa gaattctgtc tgccgcaccc gacccggcac tttcttccgt
     4441 ctttgcttcc actcgcctga gcatctccag attcgtaagt aaatacgaaa tattcttctc
     4501 tacgttttca gccgctgcct ggatgacaga tgcaggcgtg ttgatctcat gtgcaatgcc
     4561 cgccaccatc accccgagag atgccatctt ctcagattgg accagctgtg attgcgtgct
     4621 ctccaactcc tgggttcttt cgcgtacttt ctcttctaga tgacccagaa tcccttcgag
     4681 cctggcatac agaatcgaat tggaaagaga catcactccg aggcttctga cttcttctat
     4741 gagagctgtc tcccggcggc caagcttcac ggatggaggt ggtgcaagaa aaatgactcc
     4801 aaccagagat tcattgagag caagggggat gagcactctt gaattcgtaa gtgtaaaaaa
     4861 gcgatgcaac aattcacgag catcgtctgt aagatcctca tcggaggaaa tggtctctac
     4921 gtggcgaacc tggggatgtt ctccgaaata agcgatcgca ggatccatca aagaaaatct
     4981 gggctccaca cctggctgat ctggccacac agaaaatgtt ccctcttcct ctttccagat
     5041 gtagatgccc gctttctcgg aatcgagcgc ctcggttaga aattcgagca cggcccggca
     5101 tacatcacgg gtcgcacgaa gctcgatcat ggatcgttta aaatcctcaa gcctgcgcct
     5161 ttcctgcctg aatgtgtttt ttactttgct catgccgcgt gctcttagta tatcggacct
     5221 gtgagcgccg aatccggaag ctttgaggca gttgtttcca gtgtattcgg ggcttactat
     5281 gacatgctcg tactccctga ttgcctcgaa accaggaggg gcagacttcg cggacggctt
     5341 cgcctggcag ggcgcaaaaa cgaagagtcc tgggatcaca ttccagaacg ccacctgatc
     5401 ctcgtgggcg atcgtgttca ctgcgtgcag gaaaatgacg aaagcctcat agaaagcaga
     5461 cgcgaaagaa aaaacgtact gtttcgatcg tctagattcg agcaacaggg cctgggcgca
     5521 aacattgacc gcgcagttgt agtgatgagc ttcgcctacc cggatgtgtc ccaggggttc
     5581 attgatcgat tcctcgccag ctgccatgcc ggtggagtag aacctgtcat actctttaca
     5641 aaatcagatc ttacagacga tgaacttcgt gaaggatgcc tggagctcta ccgcggtctt
     5701 ggctactccg ccttcgcagt gaacttgctc gatccaaagg gagaagactg ggagtctatt
     5761 tgctctctcc tttccaatgg aatttcactt ttctccggta gatcaggcac gggcaagtcg
     5821 actcttctca atcgccttct tggaaacgaa aacatggcca cgggcgcggt atcagaatcc
     5881 acaaagaaag gcaggcacac aactaccaac tccaccatgg tgcgagatcc aaaaacgggc
     5941 gctctctaca ttgacactcc gggtgtgaag gaatggggcg taatgaacct ggacagaaag
     6001 gacatcttcg aaagttttcc tgaaattcgc gcggccgcca ctctatgtaa attcagagac
     6061 tgcctccacg aaactggcgc agaaggttgt gccgtggcag aaaagctcga aaaacccggt
     6121 gaatttcacc ctggcagaat cctgagcctg aaatccatgc tcgattccct ggaaaccggg
     6181 gaaagaatcc gtaagggaga ctacataaaa gcaacgggaa gaatgcgaca gggaagcaag
     6241 tatgccttga agaatcgccc ccctgagcac cgatgatgaa tctatgcgga gctggctagc
     6301 cctggcattc ttttgtcttt cccacgcagt ctacgcagag tgcgatcggg aatgggtgcc
     6361 cggcaattca ctggacctga gcggcagctg gaagtatgcc caggcgggag aagcgtcttc
     6421 gcctgaacta gacgactcag gctggaagcg cacgagtgtc cctggactgt ggggaaagaa
     6481 tcccgcactg gcgaactacc gcggcgaatt gtggatgcgc tgccacgtca cactcaacgg
     6541 cgaaaaacca gaaaacgtag cactcaacct gggtctggta gaggacatgg acgaagccta
     6601 cttcaatgga aacctggtag gaaggactgg ctcattcgcg cgaaacacgg tagacatcga
     6661 aacagaaaga ctctatgcac ttccagcagc gctctggcgt gagggagaca atgtaattgc
     6721 agttcgcatt cgcggaagct ctactgaatc cggcatccat gaccaggtgc gaattgaaaa
     6781 cgagcagttg gctgcgcgaa gaaacttcct gaaaaatgtt ccggcgatcg tttgctctgt
     6841 gctttacatt cttacttctg gcttcttcct gctcttctac gttttcttcc ggcacagata
     6901 cgaacatatg ttcttcgcac tgttctctgc ctttctagga ttctatcatc tgatcagaac
     6961 caacctgagg ctcgacattt tcacatcgtt tgagttctcc tatcaagtag aactggccct
     7021 tctcttctgc cttccaggac tgttcatcaa ctatctcatc tatttgctga agctcaagcg
     7081 tccaccgatc gtatgggctc tcatggggtt cagtctgatc ctcgtgattg gagcgctctt
     7141 gtccggtgca ggcaagtggc ctatcaaaca ggcgtggcaa
//
LOCUS       DAORFF010000132         7168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5270_length_7168_cov_4.220441, whole genome shotgun sequence.
ACCESSION   DAORFF010000132 DAORFF010000000
VERSION     DAORFF010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5270_length_7168_cov_4.220441"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PKK76_07005"
     CDS             <1..236
                     /locus_tag="PKK76_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68256.1"
                     /translation="SLPASVGSYFYLRRENEKHRYRLHQDNQRGLAALGLLVYAGQCV
                     DAVFFSSPRAKASFQFSPDAQGFRSQVALTFFY"
     gene            253..870
                     /locus_tag="PKK76_07010"
     CDS             253..870
                     /locus_tag="PKK76_07010"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNL68257.1"
                     /translation="MLVLLVLIASCSKPFPEADSLRLLVFARAQDWNKYIFITTATHD
                     GNFAAGFATAIAGADDFCAQQKSSSAPWLPGFSAEYRALLTDGATRRASLTADVGDGQ
                     TGWVLAPNTTYYRPDGMVLFTTNAVALYNMNTGLRNPFTSDASAAARWWTGLDLGWET
                     LLTCSSWTSSVANGTRGQGGTTTNASLSAASNGGGTPIRLLCVRQ"
     gene            complement(896..2263)
                     /locus_tag="PKK76_07015"
     CDS             complement(896..2263)
                     /locus_tag="PKK76_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL68258.1"
                     /translation="MANPHERQILTSTEKRKIWNVIFASSAGTVIEWYDFYIFGALAT
                     VISGQFFPKDHPTAGFLATLATFATGFVVRPFGAIVFGRIGDLVGRKYTFLLTLLIMG
                     GSTFAIGLLPSFEQVGVLAPILLVSLRLLQGLALGGEYGGAATYVAEHSPDARRGFYT
                     SFIQTTATLGLFVSLGVILGTRLVLGEKDFGTWGWRVPFLLSVLLVITSYYIRLKLRE
                     SPVFVKLKEEGNTSTSPLKDSFGNKANLKLVILALFGATAGQGCVWYTGQFYALTFLQ
                     KTLNIEFVTSNVIVALALVFATPFFVFFGSLSDKIGRKRIMMLGCALAALTYVPIYMA
                     MKEIGNPDNATASHTVSLQAAGLLVILVFIQVIYVTMVYGPIAAFLVELFPTRIRYTS
                     MSLPYHIGNGVFGGLTPFIATAVVASTGNPFSGLVYPIAVATLTLIVGGLYLKDHRHM
                     KISSF"
     gene            complement(2305..3594)
                     /locus_tag="PKK76_07020"
     CDS             complement(2305..3594)
                     /locus_tag="PKK76_07020"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNL68259.1"
                     /translation="MKSLRSRLSLAMIAVLIAFFTAQWWFVSAAILDVAESQMRSRLA
                     HDAETIASALTWKNGQPELPPEKTNTEYRRAYSGHYYRVTVGTNEIRSRSLWDFQPAD
                     KSCSSRRSPGPMGQILIVHCLEIRKNGNEVSIWTAEETTDLDSSVSIFQKRYTIITLV
                     AISAILIAQILAVYAGFAPLRSAVVALRKHGSASLHRTLPSEIQPFMEEIKRLVSTLQ
                     SRVDISRKTAANLVHEQKTQLAAIHSSLSSIRDKQPAPDVLVDVSEVAARAANLKDIA
                     ERHMTRSAIAGAISAGTPFDWKKDLDDLTKTMLRVYADKGPRIEVKHNQSPQWTMERQ
                     DAIELFGILLDNACRFCTGQVVIGLDTSEVTIEDDGPGCTEEQMKQLSLRGARIDEFT
                     EGGLGLSIARELAESYQMTLSFSRSPSGGLRVSIRNS"
     gene            complement(3591..4262)
                     /locus_tag="PKK76_07025"
     CDS             complement(3591..4262)
                     /locus_tag="PKK76_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNL68260.1"
                     /translation="MRILLVEDDRSILHSVRDKLQADGYQVDTAENGNDADTLGQTAQ
                     YEAILLDLGLPDRGGLALLANWRRRGIQTPVVIITARGSWTEKVEGFNAGADDYIAKP
                     FQYEELLARLRAVLRRANLASQGRRLTRGKIDLLEDSRTVVADGKSSELTAHEFKVLR
                     YLMLHAGIVVSKAKLEEQIYEDDQDHESNVLEVYISRLRKKVGEDRIITRRGQGYLFP
                     REGSE"
     gene            complement(4259..4531)
                     /locus_tag="PKK76_07030"
     CDS             complement(4259..4531)
                     /locus_tag="PKK76_07030"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNL68261.1"
                     /translation="MILKLAFALSVAITGVDGSLPAQTQEKSFVSLDRVIAEAKRRRP
                     GRLLEVELERTGGQDIYEVEILGDNRVVYKLRFNAVTLEFLGEKEE"
     gene            complement(4584..5333)
                     /locus_tag="PKK76_07035"
     CDS             complement(4584..5333)
                     /locus_tag="PKK76_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNL68262.1"
                     /translation="MKQLIWDLPTRLFHWTFAGSILAAFALGKFANEHSTLFALHMIF
                     GALAGLMLLWRIFWGFAGSKHSKFKDLLFSPRAIFEYFANVFRGKAQYFAGHNPGGAL
                     SVWIMLGLALVSVATGIGMGFFGEAVEEVHEVVTTLLIFAVAFHITGVIVATIMHNEP
                     YPIAMITGKKTAQSDESISSTRPISAAVFLVIAIAGMTYITKGFDLATRTFHAPGTSF
                     ILKFGENEKEEGGGETENAENSKDSDDDDDD"
     gene            complement(5397..6041)
                     /locus_tag="PKK76_07040"
     CDS             complement(5397..6041)
                     /locus_tag="PKK76_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNL68263.1"
                     /translation="MSNLRVFITDDHAILREGVRLILARIPGIEVVGESGDGLEALQA
                     IENLKPDLVILDISLPSLSGVELARKLRKYQPETKLLVLSRHDGPEYVEQLVKLGIHG
                     YVLKEEAGSDLIKAVEVIQRGQTYLSPRITSQLVSGFDPARSAQDPFHILSNREREIL
                     KLVAEGKSNARIGKELLISPKTVKVHRANIMKKLDVHKVADLVKYAIKAGIVEA"
     gene            complement(6034..>7168)
                     /locus_tag="PKK76_07045"
     CDS             complement(6034..>7168)
                     /locus_tag="PKK76_07045"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNL68264.1"
                     /translation="PVIAEADFRIKTGYRYCVQYFLTPFFDRGRVVGVQGVVINQTAR
                     MEALEEFQRIHDYNSRLLSSLSPVVSVGPDLRIRFANDSFAQWAGISSEKLMNRLLVP
                     VLKLKGEERDAFAANVREAGKSHKTRKGLQFRAGKSTFGYSLFPFEKDSTGIIMRDIS
                     REKELEIRIQKLNGRLIELQEEERQRIAGDLHDSVGQLILAAKLNLVSYKSDPTHFAD
                     RFDVALSIIDQASQELREIYTNLYPRSLHDLGLEATVRWHARNILETHGLKVSLEMKA
                     GTLSHELQVRIFRITQEVFANIVKHAHARSVKLKLSRNKTRIQLTISDDGKGFSPSAP
                     RSGFGLDSIRRRVESVGGEFSIESSRGHGSKFSVTLPQGEAHE"
BASE COUNT         1672 a         1814 c         1829 g         1853 t
ORIGIN      
        1 ggagcctccc tgcttccgtc ggcagttact tttacttgcg acgagagaat gagaagcatc
       61 ggtacagatt gcatcaggac aatcagagag gactggcagc gctcggtctt ctggtttacg
      121 cgggtcaatg tgtggacgca gtctttttct cttcgccgcg cgcgaaagcg agctttcaat
      181 tttcgccaga tgcgcaagga tttcgctcgc aagtagccct gactttcttc tactagaagt
      241 atgcggacgc gcatgcttgt tctcctggtt ttgattgcct cctgttcgaa accatttcca
      301 gaggcggact cgctacgcct tctggttttt gcgcgtgctc aggattggaa taagtacatc
      361 tttatcacaa cagccacgca cgacggaaat ttcgcggccg ggtttgcgac tgctattgcc
      421 ggggcagatg atttttgtgc gcaacagaaa tcttcgagcg ccccatggct tccaggattc
      481 tccgcggagt atcgagcgct cctgacggac ggcgcgacac ggcgcgcaag tctgactgcg
      541 gatgtgggtg atggacaaac cggctgggta cttgccccca atactacgta ctacagacca
      601 gatggtatgg tcttatttac taccaatgcg gttgcgcttt acaacatgaa cactggcctt
      661 cgaaatccat ttacttcgga tgcaagtgcg gcggcccgat ggtggactgg acttgacctg
      721 ggctgggaaa ccctgcttac ttgctcttcc tggacttcct ctgtagcgaa cggcacccgg
      781 ggccagggtg gcactaccac gaacgcttct ctttctgcag cgagcaatgg tggtggcacg
      841 ccgattcgtc ttctctgcgt gcgacagtaa gctgcaattc aagaagcaag caaaactaga
      901 agctggatat cttcatgtgt ctgtgatctt ttagatagag accaccaaca atgagcgtca
      961 gtgtagccac ggcgatagga tacacaagac cagagaaggg gttgcctgtg gatgcaacta
     1021 cggccgtcgc aatgaatgga gtgagtcccc cgaaaactcc atttccaatg tgatagggca
     1081 gagacatgga cgtataacgg atcctcgttg gaaacaattc cactagaaac gcggctatag
     1141 gcccgtacac catcgtcaca tagattactt gaataaatac aagaatcacg agtaacccgg
     1201 ctgcctggag cgaaactgta tgggacgcag tggcattgtc agggttgccg atttctttca
     1261 ttgccatgta aatgggaacg tatgtgagcg cggccagagc gcaacccagc atcatgattc
     1321 tctttctacc gattttgtcc gacaaagatc cgaagaatac aaagaacggc gtcgcaaaca
     1381 ccagggccag agcgacgatc acgttcgacg ttacgaattc tatgttcagt gttttctgca
     1441 agaaagtgag tgcgtaaaac tgccctgtat accacacgca gccctggccc gcggttgctc
     1501 cgaacaatgc aaggatgacc agtttcaaat tcgctttgtt tccaaaagaa tctttcagag
     1561 ggctcgtgga tgtgtttcct tcttccttca atttaacaaa tacaggcgat tctctgagtt
     1621 tgagtcttat gtagtacgaa gtaattacaa gtagaaccga aagtaggaat ggaacgcgcc
     1681 agccccacgt gccaaaatct ttttcgccga ggacgagtct ggttccaagg atcacgccca
     1741 gggaaacaaa tagacccagc gttgccgtag tctggatgaa acttgtgtag aagcctctcc
     1801 ttgcgtccgg tgagtgttct gctacgtaag tggccgcgcc gccgtattcg cctcccagcg
     1861 ccaggccctg aagaaggcgc aaggaaacca ggagtatggg ggcaagtacg ccaacctgct
     1921 cgaaagacgg caaaagacca atggcgaaag tagagccgcc catgatcagc agtgtcagca
     1981 ggaacgtgta ttttcttccg acaagatcgc ctatgcgtcc gaagacgatg gcaccaaacg
     2041 gtctgactac aaaaccggtt gcaaaagtag caagggttgc cagaaatcca gccgtcggat
     2101 gatcttttgg aaagaattgg ccggagatga ctgtggccag ggccccgaat atatagaaat
     2161 cataccattc aatgaccgtt cccgcagagg atgcgaagat cacgttccag atttttcttt
     2221 tctccgtact ggtgagaatc tgtcgctcat gcggatttgc catgcgagca ggaggtctgg
     2281 cggtcgcctt acatcaagaa gattttatga gttgcggata gaaactctga gtcctcctga
     2341 aggcgaacgc gagaatgaaa gagtcatctg gtagctttct gccagctctc gcgcgatgga
     2401 aagaccaagc ccgccctctg tgaattcgtc aattctagct ccgcgtaaag acagttgttt
     2461 catttgctcc tctgtgcatc cggggccatc gtcttcgatc gtaacttctg atgtgtcgag
     2521 tccgatgacc acctggccgg tgcagaatct gcatgcattg tcgagcagaa ttccgaagag
     2581 ctcgatggca tcctgtcttt ccatagtcca ctgtggactt tgattgtgtt ttacttctat
     2641 tcgcggtccc ttgtccgcat acactctcag catagttttc gtaagatcat ccagatcctt
     2701 tttccaatca aagggcgttc cagcagaaat ggcgcctgcg atcgcggagc gagtcatatg
     2761 tctttccgca atgtctttga ggtttgcggc acgggctgca acttcagaaa catcaacgag
     2821 cacgtcgggt gctggctgtt tgtcgcgaat agaagagagc gacgaatgaa ttgccgcgag
     2881 ttgcgtcttc tgttcgtgaa ccagattggc cgccgttttt cgcgaaatat ccacgcgcga
     2941 ttgcagtgtg ctcaccagcc gtttgatttc ttccatgaag ggttggattt cggatggtag
     3001 agtcctatgg agggatgcac tgccatgctt tcgcagggcc acgacagcgg atcgaagcgg
     3061 agcgaatcct gcgtatactg ccagtatctg tgcgatcaga atggcactga tggcgacgag
     3121 cgtgatgatc gtataacgtt tctgaaaaat gctaacagac gaatccaggt ctgttgtttc
     3181 ttctgcagtc cagatactta cttcgtttcc attttttcta atttccagac aatgaacgat
     3241 caggatctgc cccatgggtc caggacttct gcgagaactg caactcttgt ctgcgggctg
     3301 gaaatcccag agtgagcgtg agcgaatttc attcgttccg acagtgactc tgtagtaatg
     3361 gccggagtaa gcgcgtctgt attctgtatt agttttttct ggcggcaatt ccggctgacc
     3421 attcttccag gtaagcgcag aggcgatcgt ttcggcgtcg tgagcgagtc tcgaacgcat
     3481 ctggctttct gcgacgtcta gaatcgcagc ggatacaaac caccactgag ccgtgaagaa
     3541 cgcaatcagc actgcgatca tggccagtga tagtctggag cgaagcgatt tcactctgaa
     3601 ccctctctgg gaaagaggta tccctggcct cgcctggtaa tgatgcggtc ttctcctact
     3661 ttttttcgca gtcgactgat ataaacttca agaacattgc tttcgtgatc ctgatcgtct
     3721 tcgtagatct gctcttcgag ctttgccttg gatacaacga ttccggcgtg aagcatcaga
     3781 tatcgcagca ctttgaattc gtgagcggtg agttcagaag attttccgtc tgcgacgaca
     3841 gttcgtgagt cctctaaaag atctattttt ccacgtgtca gcctgcgtcc ctgtgaagca
     3901 aggttcgctc ttcgcaaaac agcgcgaagg cgagcaagaa gttcttcgta ctgaaagggc
     3961 ttggctatgt agtcgtctgc gcctgcattg aaaccctcca ctttctccgt ccaggagccg
     4021 cgcgcggtaa tgattacgac tggagtttga attcctctcc ttctccagtt tgcaagaagg
     4081 gcgagccctc cccggtccgg taatcccaga tccaggagga tcgcttcgta ctgagcggtt
     4141 tgcccgagag tgtccgcatc gtttccattt tcggctgtat ccacctggta gccgtctgcc
     4201 tgcagtttgt ctctgacaga atgcagaatg gaacgatcgt cctctacgag caagattctc
     4261 attcttcctt ttcccccagg aattccagag tgactgcgtt gaacctgagt ttatacacta
     4321 cgcgattgtc ccccaggatt tcaacttcgt atatatcttg tccgcctgtc cgctctaatt
     4381 ctacttccag gagtcgacct ggcctgcgtc gtttggcctc tgcgatcacc cggtccagag
     4441 acacgaatga cttctcttgc gtttgtgctg gcaaggagcc atctactcct gtgatggcga
     4501 ctgaaagcgc gaatgccagt ttgagaatca ttctgcttct cacagtccta tttcgcaagc
     4561 tgacatttcg cgcatcgcct tttctaatcg tcgtcgtcat catctgagtc tttggagttc
     4621 tctgcatttt cagtttctcc gccgccttcc tccttttcgt tctcgccgaa cttcagaatg
     4681 aaggaagtac ccggagcgtg aaaagtccgt gtcgcaagat cgaagccctt tgtgatgtag
     4741 gtcatgcctg caatcgcgat cactagaaac accgcggcgg agatgggacg agtgcttgag
     4801 atggattcat ctgattgagc agtcttcttg cccgtaatca tggcaatcgg atacggctca
     4861 ttgtgcatga tcgtggctac gattacgccg gtgatatgga aggccactgc gaagatgaga
     4921 agggttgtga ctacttcgtg tacttcttcg actgcttctc caaaaaatcc catgccaatt
     4981 ccggtcgcga cggaaaccag agccagacca agcatgatcc agacagacag tgcaccgcct
     5041 ggattgtggc ctgcgaaata ctgcgcctta cccctgaaga cgtttgcgaa atactcgaag
     5101 atggcgcggg gggagaagag caaatccttg aatttgctat gtttgctgcc tgcaaatccc
     5161 cagaagattc tccacagaag catgagtcct gcgagagccc cgaagatcat gtgcaatgcg
     5221 aagagggtgg agtgttcatt tgcaaatttt cccagcgcga aagcggccag aatggaacct
     5281 gcaaatgtcc agtgaaagag gcgtgtgggt aaatcccaga tcagttgttt cataggttca
     5341 ggatagtctg tgaacctgaa cgcaggctga atgtgcgaaa aaaaaatctt acgggctcag
     5401 gcctctacaa taccagcttt gatggcgtat ttcactagat ctgcgacctt gtgaacatcc
     5461 agtttcttca tgatattggc gcgatgaact ttgacagttt tgggggaaat cagcagttcc
     5521 tttccaatgc gagcgttgga ttttccttcc gcgaccagct tcagaatttc gcgttctcta
     5581 ttcgagagaa tgtgaaaagg atcctgcgca gacctggcgg gatcgaatcc ggaaacaagc
     5641 tggcttgtaa tgcgagggct caaatatgtc tgacctctct ggatgacttc caccgctttg
     5701 atcagatcac tgcctgcttc ttcttttaaa acatagccat gaatgcccag cttcacgagc
     5761 tgttctacat attccggtcc gtcgtgtctg gatagaacaa ggagctttgt ttcaggctgg
     5821 tattttctga gcttgcgcgc gagttctact ccggaaagag aaggtaggga aatatcaagt
     5881 atcaccagat caggtttaag gttttcaatt gcctgaagag cctcgaggcc gtcgccagat
     5941 tcccctacca cttcgattcc aggaatccgg gcgaggatca gtctaacgcc ttcgcgaaga
     6001 atcgcatgat catctgtgat gaaaactcta agattactca tgtgcttccc cctggggcag
     6061 agtgactgag aatttcgaac cgtgtccgcg gctcgactcg atggaaaact cgcctcccac
     6121 agattccact ctccttcgaa tgctgtccag accaaatcca gatctgggtg cgctgggaga
     6181 gaaacccttt ccatcgtctg agatagtgag ctggatgcgg gttttgttgc gacttagttt
     6241 aagcttcacg cttcgcgcgt gagcatgttt gacaatattt gcaaaaactt cctgcgtgat
     6301 ccgaaagata cgcacctgca attcatgcga aagggttcct gctttcattt cgaggctcac
     6361 tttcagaccg tgagtttcga gaatgttgcg agcatgccat cgaacggttg cctcgagccc
     6421 gaggtcatgc aacgatctgg gatacaggtt cgtgtagatt tcacgaagct cctggcttgc
     6481 ctggtcaatg atggacagag caacgtcgaa tcgatctgcg aagtgagtgg gatcactttt
     6541 ataggaaacc agattgagtt ttgcggccag gatcaattgt ccgacgctat catgaaggtc
     6601 cccggcaatg cgttgtcttt cttcttcctg gagttcaatg agtctaccgt tcaatttttg
     6661 aatgcgaatc tccagttcct tttctctgga gatatcgcgc atgatgattc cagtgctatc
     6721 tttttcgaat ggaaagagtg agtacccaaa tgtggacttc ccggcgcgaa actgcagacc
     6781 ctttcttgtt ttgtgactct tgcccgcctc gcgaacgtta gctgcaaatg cgtcccgctc
     6841 ttcccctttg agtttcaaaa ccggaacaag gagcctgttc atcaatttct ccgaggagat
     6901 tcctgcccac tgcgcgaagg agtcatttgc aaaccgtatg cgaagatcgg ggcctacgct
     6961 tacaaccgga gaaagactcg acagcaggcg agaattgtag tcgtgaattc tctggaattc
     7021 ttccagtgcc tccatgcgcg ctgtctgatt gatcacgact ccctgtactc cgaccactct
     7081 gcctcggtca aagaacggag ttagaaagta ttgaacacag taccgatatc cggtcttgat
     7141 tctgaaatct gcttccgcaa tcacaggt
//
LOCUS       DAORFF010000133         7157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5285_length_7157_cov_3.627429, whole genome shotgun sequence.
ACCESSION   DAORFF010000133 DAORFF010000000
VERSION     DAORFF010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5285_length_7157_cov_3.627429"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PKK76_07050"
     CDS             complement(<1..239)
                     /locus_tag="PKK76_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68265.1"
                     /translation="MGGEKTQEPAVRDLVQEKLSLVVKSGKYDQAHGTVAYSFGHAGR
                     AFPVCFGSAMCLALLFGSGQLIRSGVPFMLIAALTA"
     gene            complement(281..1003)
                     /locus_tag="PKK76_07055"
     CDS             complement(281..1003)
                     /locus_tag="PKK76_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNL68266.1"
                     /translation="MAQAKARKKSKSAVRAKPAAKKSAKAPAKKAAKKTTAWPQAFQK
                     LIQKYGKEPHPLNYSSPYELLVKVVLSAQTNDAFINKIAPEFLKAYPDFRSIARGSAE
                     DLYPYLKGVRNFANKSRWLFAIASKLEESEQIPRTMEALTELPGIGRKSASVLLRELG
                     GKGAGVIVDIHVLRVAPRLGISKQEDAGKMEKEIMAVLPESMWADAGMCISFLGRDVC
                     RPTAPDCGNCVMQKVCEYAAKK"
     gene            complement(1005..1973)
                     /locus_tag="PKK76_07060"
     CDS             complement(1005..1973)
                     /locus_tag="PKK76_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNL68267.1"
                     /translation="MNPFSLFEKSYKMQIDELQRNASLIDADSVARAIDLIQNCKGKL
                     VLSGMGKSGLIAQKLAATFSSTGTPSFFLHPGEALHGDLGAVQKNDVLLVLAKSGESE
                     EVILMLQAIRKMGNPVISILGKSDSTAGRMSDVIIHASVSREADPLNLAPTASSTVSL
                     VVGDALASAVCELRGFKQENFALLHPAGQLGKRLLLKVEDLLILDRGLPVVGEHASMA
                     ELLEVESKPNLGGVMIADESGKLIGLITDGDIRRSILKFGDVLKCKIVDIMTRNPIHI
                     QTGTSALSALDLMENRPSPVSVLPVVDSDKKPVGLLRLHDLLRSGL"
     gene            complement(1998..2534)
                     /locus_tag="PKK76_07065"
     CDS             complement(1998..2534)
                     /locus_tag="PKK76_07065"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HNL68268.1"
                     /translation="MIQAQPSGMSEETRWYALYTMPQCEKRISLFLSKRKIDYYLPLV
                     PKKRKWSDRIKVVEFPLFPGYLFIHMPFYQRRLDVLLHPSALRFVQHEGRPAIIPDDD
                     IGYLHILVDNANDLQAHPEENLPEGQKVIVRSGPFKGIRGVVARVKNKDRIFVKLEHC
                     ALMASAEIDILDVEKLII"
     gene            complement(2531..3544)
                     /locus_tag="PKK76_07070"
     CDS             complement(2531..3544)
                     /locus_tag="PKK76_07070"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNL68269.1"
                     /translation="MMETEHHRIGQGQKPYLIAEIGLNHNGDPALAKKLVDAAGTSGA
                     HAVKFQLYDSDHFIHEEARLGDGSLREFFRQFELSSAQWHDLAGYARSCGLDFFCSVF
                     DFPSIELYASLSPRLIKIASCDLTNRPLIQECAHRLPGVPLMIATGTADEAEVESLMA
                     WIRSLSSPVILLECVSHYPANPDEYNIGLIQQWNQKFDVPVGISDHTSGIGVSLAACL
                     LGAVAVERHFTTSHDLPGPDQKISLTAETFALMRNEIDAALSTRRSGNKQVQKSESAP
                     RKFGRRGVYAARDIKSGSTLSDQDLLFMRPGGDGVRPEENLAGRTLKKDKKTGDMILG
                     EDL"
     gene            3584..5062
                     /locus_tag="PKK76_07075"
     CDS             3584..5062
                     /locus_tag="PKK76_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNL68270.1"
                     /translation="MFPMLEKSILPEGRPPNIGALFLDQCHIYDESVALSFRDGSDVK
                     TVTYGKLAKIVRDIAGGVASRGLGPGARALVVAHNTPEWVFAFYAIHVCGGVDVCVNP
                     ALPHSVIIQLARQIKPAVALVEDPDLGELLEQEGKIKVIFLNWKKEGYKNLRSLRQRF
                     TFLGQGHKRRQPDHEASIVFKKSDPELQQPGGLILSHGSIIKNVEAYTSSLPGTQQDR
                     LMIRAPLWHSSGRLGMLTALSRGIELQLSRKDVLDDCEALQPTLLLAHPSTLDAIETR
                     VFEGGESGGMLGNLFMRAYIFFGAEAMRAMNHLTGQTAVFGSSEKQSFVGLVIDLVLL
                     IFLLPVKILGDGLFGTRLRKRFGGSLRCVLTGGAPVRGRLDLFFGMLGVSVLAAYFKT
                     EACHVIASRILEYTGQRSRLQPGTVGPPLAGAEIKILQAGSDVSSTPGAVGEIMIRGD
                     FLFQGYLSGQSDVFDASGWFYTGDRGSLSNQGELRIARRDER"
     gene            complement(5075..5626)
                     /locus_tag="PKK76_07080"
     CDS             complement(5075..5626)
                     /locus_tag="PKK76_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68271.1"
                     /translation="MLIRHIVWILCVAAWTGCSSQTAIVNSLPRDAKSLADLLNDYRI
                     KNGLPPIRSSVSLTRVATAHVTDLQNHFEKGKLCNLHSWSSHGNWTPCCYTGEQVQCM
                     WNKPREITKYKADGYEITVSAFTTARPDAMTPWLAVDRWAHSDPHRAVILNEGQWANF
                     PWKAMGAAESDNYASAWFGQEAD"
     gene            5842..6414
                     /locus_tag="PKK76_07085"
     CDS             5842..6414
                     /locus_tag="PKK76_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNL68272.1"
                     /translation="MHFPTPRSGMPQREGFGLPPGALKEMGRDKYLDAMYEGIKKDFQ
                     DAGIKLTPIENGFHATGVNIEKVKDPVTKKTKELLVSIDGDISFAHGSSLVTPQARAV
                     IDRVAKAMAAYPDINVTVGGHTDSTGFKQLNVVLSKERAQSTADLLVQLGKIDRKRIV
                     EVKGFADDQKIVDTMLAEVRNRRVEIRMAP"
     gene            6414..>7157
                     /locus_tag="PKK76_07090"
     CDS             6414..>7157
                     /locus_tag="PKK76_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68273.1"
                     /translation="MRLFLIALLITGFPLFAQNPPKREPQKTEPPKTEPPKTEPRKTD
                     PPKTEPPRVEPVKTEEEKAKESEKIDTNAPVLNPYLLRRFEAFRFRVMAGAGYQSIKP
                     SILSEAGPAWQLNSFIRAASNGTSQPALLIQDTKKVPAYRYKVSGDVAYKDRLFFDYE
                     RQWTKQDYSKKYPTRVTFAAPGNSTYETALYEGLRMLQYKEVTNNFSLTYLHPLGKGV
                     KLGGYLEREIYHENLEISMGSLSLTKTNTL"
BASE COUNT         1678 a         1801 c         1867 g         1811 t
ORIGIN      
        1 gccgtcaagg cagctatgag catgaaagga acaccggatc gaatcaactg gcccgaacca
       61 aataacagcg cgaggcacat cgcacttccg aaacagacgg ggaaagctcg ccccgcatgt
      121 ccaaatgaat aggccactgt tccgtgcgcc tgatcgtatt tccctgactt gacaaccagg
      181 gacagcttct cttgcacaag atcccgtaca gccggctcct gtgtcttctc ccctcccaca
      241 ggttctccgc gtcagtcttg atcgcccaac gttcatggat ctattttttt gccgcgtact
      301 cgcagacttt ctgcatgacg caatttccac agtccggagc agttggtctg catacatctc
      361 tgcccaggaa tgaaatgcac attcctgcgt ctgcccacat ggactccggc agaactgcca
      421 tgatttcctt ctccattttt cccgcgtctt cctgttttga aattccaaga cgaggcgcca
      481 ctctcagaac gtgaatgtct acgatcactc cagcaccctt gccgcccagc tctcgaagaa
      541 gaacgctcgc agatttgcgt ccgattcccg gcagttccgt gagcgcttcc atcgtgcgag
      601 gaatttgttc actctcttcg agcttcgagg cgatagcgaa aagccatctg gacttatttg
      661 cgaaatttcg aactcccttc aaataaggat acaaatcctc tgcactgcca cgagcgatgg
      721 agcggaagtc aggataggct ttcaggaatt caggggcgat tttgttgatg aatgcgtcgt
      781 ttgtttgcgc cgacagaacc actttgacta gaagttcgta gggactggag tagttgaggg
      841 gatgaggttc ttttccgtat ttctggatta gtttttgaaa cgcctggggc cacgctgtcg
      901 ttttcttcgc agccttcttt gcgggagctt tcgcactctt ttttgccgca ggttttgctc
      961 tgacggctga tttgcttttt tttcgggcct tcgcctgcgc catgttatag accggacctc
     1021 agcaggtcat gcaggcgaag aaggcccaca ggttttttat ctgaatccac aacgggcaaa
     1081 acggaaacgg gagacggacg gttctccatg aggtcaagcg cggatagcgc acttgtgcct
     1141 gtttgaatgt gaatcgggtt tcgcgtcatg atgtctacga tcttgcattt aagaacgtct
     1201 ccaaatttga ggatggatcg ccggatgtct ccgtccgtga tcaatccaat gagttttccc
     1261 gattcgtctg cgatcatgac tccgccgagg tttggtttgg actctacctc tagaagctct
     1321 gccatgcttg cgtgttcgcc aacgacaggc aggcctctat ctaggatcaa aagatcttcc
     1381 actttcaaga gaagccgttt gcccagctgc cctgccggat gaagaagagc aaaattttcc
     1441 tgtttgaatc cacgaagttc gcagacagcg ctggcaagtg catctcctac aaccagagag
     1501 acggtgctgg atgcagtggg cgcgagattg agaggatccg cttcgcggct cacggacgca
     1561 tggatgatga catctgacat tcttcctgcc gtggaatctg attttccaag aatggaaatc
     1621 acagggtttc ccattttgcg aattgcctgg agcatcaaaa ttacttcttc ggattctcca
     1681 ctcttcgcaa gcacaagcaa cacatcgttt ttctgaactg ctccgagatc tccgtggagc
     1741 gcttctcctg gatgaaggaa aaacgatggt gtgcccgtgg atgaaaaggt agccgcgagc
     1801 ttttgcgcga tgagccctga ctttcccata ccgctgagta caagtttgcc tttgcagttc
     1861 tgaatcagat cgattgcgcg cgcaacagaa tcagcgtcga tgaggcttgc atttctttgc
     1921 agctcgtcga tttgcatttt gtagcttttt tcaaagagtg aaaatgggtt cattggcgac
     1981 cttcgtgaaa cggcttgtta tatgatgagt ttctcaacat ccaggatgtc aatttccgcg
     2041 ctcgccataa gggcgcagtg ttccagttta acaaaaattc tgtctttgtt ttttactcgc
     2101 gccactactc cgcgaattcc tttgaagggc ccggatcgca cgatgacttt ctgtccctcc
     2161 ggcaaattct cttccggatg ggcctggaga tcattggcgt tgtctaccag tatatggagg
     2221 tagccaatgt catcatcagg aatgatggcc ggtcttccct cgtgctgcac aaagcgaaga
     2281 gcgcttggat gcaagagtac gtccaatcgt ctctgataga agggcatgtg aatgaaaagg
     2341 tagccgggga agagtggaaa ttccactacc ttgatgcgat ctgaccactt cctttttttg
     2401 ggaaccagag gaaggtagta gtcgatcttt ctcttggaaa ggaacagaga aattctcttc
     2461 tcacattgag gcattgtata cagggcatac catctggttt cttccgacat cccggaaggt
     2521 tgcgcctgaa tcacaggtcc tctccgagga tcatgtctcc ggttttctta tctttcttga
     2581 gagtgcgccc cgcaaggttt tcctccggac ggactccgtc tccacccggt ctcatgaaaa
     2641 gcaaatcttg atcgcttaac gtggatccgc tcttgatgtc gcgcgccgca tagactccac
     2701 gccttccgaa tttgcgagga gcggattctg atttctgtac ctgtttgttt ccgcttctgc
     2761 gtgtgctgag tgcggcatca atttcatttc gcatcaaggc gaaagtctca gcagtcagcg
     2821 aaattttttg atccgggccg ggcagatcgt gcgaagtcgt gaaatgccgt tctactgcca
     2881 cagcacccag aaggcaggcg gccaggctca ctcctattcc ggaagtgtga tctgatattc
     2941 caacaggaac gtcgaacttc tgattccatt gttgtatgag gccaatgttg tactcatctg
     3001 gattcgcagg gtaatgcgaa acgcattcga gcaggatgac cggcgaagag agtgagcgaa
     3061 tccaggccat gagactctct acttctgctt catccgcagt gcccgttgca atcatcagcg
     3121 gaacgccagg aaggcgatgc gcgcattcct ggatcaatgg acggttcgta agatcgcagg
     3181 aagcgatttt gatcagtcga ggagagagac tcgcatagag ctcaatggac ggaaaatcaa
     3241 aaacagaaca aaagaaatcc agcccgcatg agcgagcata accagcaaga tcgtgccatt
     3301 gtgcagatga tagctcaaat tgccggaaaa attcgcgcag agatccatcc cccagccttg
     3361 cttcctcgtg gatgaaatgg tccgaatcat agagctggaa tttgactgca tgcgcgcctg
     3421 aagttccggc cgcatccaca agtttcttcg cgagagccgg gtctccgttg tgattgaggc
     3481 caatttctgc gatgaggtaa ggcttctggc cctggcctat tctgtgatgc tccgtctcca
     3541 tcatctgctg taccgattga ctcggcacgc ctcccgcgca agcatgtttc cgatgctaga
     3601 gaagagcatt cttcctgaag ggaggccccc caacattggc gcgctgtttc tcgatcaatg
     3661 tcatatttac gatgagtccg tggctctttc gtttcgcgat ggatcagatg taaagacagt
     3721 cacctatggc aagctcgcta aaatcgtaag ggacattgcg ggtggagtgg cctcgcgagg
     3781 tctgggcccc ggggcgcgtg ctctcgtggt tgctcacaat acgccggaat gggtcttcgc
     3841 attttacgcc attcatgtgt gcggtggagt ggacgtgtgc gtgaatcctg ctcttcctca
     3901 ttctgtcatc attcagctgg cgcgccagat caaacctgcc gtggccttag ttgaggatcc
     3961 agatctggga gaactgctgg agcaagaggg caagatcaaa gttatctttt tgaactggaa
     4021 gaaggaaggt tacaaaaatt taagatcgct caggcagagg tttacgtttc tagggcaggg
     4081 tcacaagcgc aggcagccgg accatgaagc cagtattgtt ttcaagaaaa gtgatccgga
     4141 attgcagcaa cccggaggcc tcatcctttc ccatggttct atcatcaaga atgtggaagc
     4201 atacacttct tctttgccag gtacacagca ggatcgcctg atgattcgcg cacccctctg
     4261 gcattcctcc ggaagattgg gaatgttgac tgccctttcc cgtggcattg aactgcaact
     4321 ttcgcgcaag gatgttctgg acgattgtga agcacttcag cctactttac tcctggctca
     4381 tccatctacg ttagacgcca ttgagactcg tgtttttgag ggtggggaat caggcggaat
     4441 gctgggaaat cttttcatgc gcgcgtacat tttctttggc gcggaagcca tgcgtgccat
     4501 gaatcatctc acgggccaga cagctgtgtt tggatcttcg gaaaagcaat cctttgtcgg
     4561 gcttgtcatt gacctggtgc ttctgatttt tcttttacct gtcaagattc tcggggatgg
     4621 cctttttggc acacgccttc gaaaacgttt tggtgggagt ttgcgctgcg tgcttacggg
     4681 cggagctccc gtgcgaggga gactggattt gttcttcggg atgcttggcg tttctgttct
     4741 ggcagcgtat ttcaagacgg aagcctgtca cgtgatcgcc agccggattc tggaatacac
     4801 aggacaaaga agcagactgc agccgggcac ggttggtccg cctctggcgg gtgcggaaat
     4861 caagattctt caggcgggtt ctgacgtttc gtctactcca ggtgctgtgg gcgagatcat
     4921 gattcgcggg gattttcttt ttcagggata cctgagtggc cagagtgatg tgtttgatgc
     4981 gagcggctgg ttctacacag gcgatagagg gagtctatcc aatcaggggg aactaagaat
     5041 tgcgcgccgg gatgaacgtt aggcgattag actgtcaatc tgcttcttga ccaaaccagg
     5101 cagacgcata gttgtctgat tccgcagccc ccattgcctt ccagggaaaa ttcgcccact
     5161 ggccctcatt taagatcacc gccctgtgcg ggtcactgtg ggcccatcga tccacggcca
     5221 gccagggagt catcgcgtcc ggccttgcag tcgtgaaggc agaaacagtg atttcgtagc
     5281 cgtctgcttt atatttagtg atttcgcgcg gcttattcca catacactgg acctgttctc
     5341 ctgtatagca gcaaggagtc cagttaccat gcgaagacca ggagtgaaga ttgcagagtt
     5401 tgcccttttc aaagtgattt tgtaagtcgg ttacgtgtgc ggtggccacg cgcgtgagtg
     5461 aaactgaact gcgaatgggg ggaaggccgt tcttgattct gtagtcattg agcaaatccg
     5521 ccagagattt cgcatcgcga gggagagagt tcacgattgc agtctggctg gaacatcctg
     5581 tccacgccgc gacgcataag atccacacaa tgtgacgaat gagcataggg acggagtccg
     5641 cgccacgcga aacggggcaa ccaaaagatt tttgacggat tggggctcat cgtgaacgtg
     5701 tccgtaactt tcatggttgc attttctaaa cgtttggtgt tttgcgcgat ttatgtcctt
     5761 ttgctagttt tcgtcgcgtg cggtactaca ggatctcgca ttcgctcggc gttgacggat
     5821 tgcgcctgtt atgaagaaga tgtgcacttc ccaactccgc gcagtggtat gccgcagcgc
     5881 gaaggcttcg gtcttcctcc cggcgcactc aaggaaatgg gccgcgacaa gtacctcgat
     5941 gcgatgtacg aaggtatcaa gaaagatttc caggatgcag gaatcaagct cacaccgatt
     6001 gaaaacggat tccatgcaac gggcgtgaac attgaaaaag tcaaagaccc ggttacgaaa
     6061 aagacaaagg aacttctcgt ttctattgat ggcgatatca gtttcgcgca cggaagttcg
     6121 ctcgtaacgc ctcaggcacg cgccgtgatc gatcgcgtgg ccaaagcgat ggccgcgtat
     6181 ccagatatca atgtgactgt aggcggacac actgactcta caggtttcaa acaacttaac
     6241 gtagttctat ctaaagaaag agcgcaatct acagcggacc ttctggttca gctgggcaag
     6301 attgatcgca agagaattgt ggaagtaaaa ggtttcgcag acgaccagaa gattgtggac
     6361 accatgcttg cggaagttcg taaccgccgc gttgaaatca ggatggcgcc ctgatgcgac
     6421 tttttcttat cgcattgctc atcaccggtt ttcctctgtt cgcgcagaat ccaccaaagc
     6481 gtgaaccaca gaaaacggag cccccgaaaa cagaacctcc aaaaacggaa cccagaaaga
     6541 cggatcctcc taaaacagag ccgcccagag tggaaccagt caagacagaa gaagaaaaag
     6601 caaaagagtc agagaaaatc gatacgaatg ctcctgtttt gaatccgtat cttcttcgtc
     6661 gtttcgaggc atttcgtttt cgagttatgg ccggtgcagg ttatcagtcc atcaagccat
     6721 cgattttgtc cgaagcgggt cctgcgtggc aactcaattc attcattcgc gccgcgtcca
     6781 atggcacatc gcaaccagcg cttctcattc aggacacaaa gaaagttccc gcgtacagat
     6841 acaaagtgag cggggacgta gcatacaaag acagattgtt ttttgactat gaacggcaat
     6901 ggaccaaaca ggactacagc aagaaatatc caacgcgcgt tacctttgcc gcacctggaa
     6961 acagcacgta tgaaacggct ctttacgagg ggctgcgaat gctacagtac aaagaggtaa
     7021 ccaacaactt cagccttacc taccttcatc cactcgggaa gggcgtgaag ctgggcgggt
     7081 atctcgaaag agaaatctat catgagaatc tggaaattag catgggttct ctttctctca
     7141 caaaaacaaa cactctt
//
LOCUS       DAORFF010000134         7155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5287_length_7155_cov_4.343662, whole genome shotgun sequence.
ACCESSION   DAORFF010000134 DAORFF010000000
VERSION     DAORFF010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5287_length_7155_cov_4.343662"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PKK76_07095"
     CDS             <1..840
                     /locus_tag="PKK76_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HNL68274.1"
                     /translation="TVAGNMPVAYLGKKGSFASLVARQRYPDAETVSQPTIGDLVTYV
                     KEGADRMGVVPIENSSGGIIFDTVDAFVDDSFNLFILEETRIRVRYALLGKSGQPIQT
                     ILSHYAALRHCAAWIRSTYPAVRTQPVASTSEAAAMAAAGSQLAAISTRESANEYGLD
                     VLHFPIMDSVPNITQFFTIGHAPVNYSGSGGNTSLVATLDNSPGSLFRFLKPFHDLGV
                     NLNRIQSRTIPGDSSACKFFVGLEGVESETNVGRAIELARPLTVALQLLGSYPRLMDP
                     YES"
     gene            complement(848..2542)
                     /locus_tag="PKK76_07100"
     CDS             complement(848..2542)
                     /locus_tag="PKK76_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNL68275.1"
                     /translation="MARFCLLWLIPFVLHAGPALRVLEVDSSHFPNVEILAELGEGEG
                     TPQFTLRDGAPITPSITASRKEADRLVLAVVFDATGSIPAADYRRAQNGAGVFLSSLT
                     ESDRTALFQINGGSINISGFVPGGKTISEQIAQMKRVGRTTRIFDALHSGINAAREGI
                     LRNQNTESSSKQIAKAAVLLFTDGRDEASYLNEQDCLELAELGTKLRVPVYTVLYGHP
                     RNQRVFQRLSMRTGGKLITMLTPDTMRNLSRSIRLLKDETFRLSYKAPEGAGPRNIVL
                     SVKRGTTIDEDTASYNAPSVSIPHSEGPWYNSIPWGNVLLGVLSFLLFVAAVVALILV
                     LKQKHDAAKATNPAVVSASDSPTAHVKLPEGETEKVDIVEGIPAEDMPHPAVQYLREN
                     AYRSLQEALRGAPRYAAASLIRMKDGMSREYDLFLENTALGSGRWASIRVEDSGAAKV
                     HAKVKRVDSRYVLYDLMGGSSVFLNGKKLLRPRGLHDGDEIKLGRSVFQFRGKGKGNA
                     AAPDDRTETVQGKSNPASSMDSTQAMISDGSEDPADYSDTMPEEDLHDSDFLQKAE"
     gene            complement(2564..2827)
                     /locus_tag="PKK76_07105"
     CDS             complement(2564..2827)
                     /locus_tag="PKK76_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68276.1"
                     /translation="MIRDILYSIIYSGIGIYLVLIALGFLHRGVLGEMTGARRLAAWI
                     MALGFLGLGGHHAWQYYRFSSPEGRQILEKMKETEGNVIIGSP"
     gene            complement(2824..3789)
                     /locus_tag="PKK76_07110"
     CDS             complement(2824..3789)
                     /locus_tag="PKK76_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNL68277.1"
                     /translation="MQSGHLGIDLGGSNIFSVVFDDSYHPVTQSKIDTEAKEGYNHVI
                     GRLRGQIDALIASCESKGIRIETIGLGVPGVVSKDTDLVRVAPNLNWENRHPLQDLGL
                     LVQRDIRSVLVNDVNAGLMGELARMQHKPSIAIAFFCGTGIGGAIAINGELVLGRDGG
                     AGEVGHIIVRDGGRKTDSGLRGSLESYIGKWALNKRVDRYIGMKKKTVLRDLIDYNLR
                     KTPIKSSSLKKAYEKGDGFAQALMNEYYCKFLAVGISQAVNMLNPDLVILGGGIMEAM
                     GVKLLPHVQSKLAMRCINPLPQIRLAELGDMAGPMGAAYLARKKQ"
     gene            complement(3839..4447)
                     /gene="recR"
                     /locus_tag="PKK76_07115"
     CDS             complement(3839..4447)
                     /gene="recR"
                     /locus_tag="PKK76_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNL68278.1"
                     /translation="MLFPSLENAARLLSSLPGIGSRSALRIAFHLIRSDSDKSEDLAE
                     ALIRLKKEVRFCSLCGGLSDADLCPICRDETRDRSQLCIVEEPGDVYAIEKTGEFKGL
                     YHVLMGAISPLDGIGPGDLRISELESRIASDDRINEVLLATNPTLEGDVTADYIANAL
                     TGRNLNITRISHGVPTGAAIEYADRVTLGRSIRQRQDYRRGD"
     gene            complement(4447..4770)
                     /locus_tag="PKK76_07120"
     CDS             complement(4447..4770)
                     /locus_tag="PKK76_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNL68279.1"
                     /translation="MNFSEMFKNLGQMKARAEELQTKMARLQITGEAGAGMVRVTVNG
                     EGTVLKIQIEPNLLNPKDKEMLEELVISATNEAVKKSREAMAHEMKSLTGGIPGLDEM
                     FRQGS"
     gene            complement(4778..6046)
                     /gene="dnaX"
                     /locus_tag="PKK76_07125"
     CDS             complement(4778..6046)
                     /gene="dnaX"
                     /locus_tag="PKK76_07125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNL68280.1"
                     /translation="MTEVAHLVLARKYRPRVFKEVVEQGLAVTALKNAADHGRIGSAY
                     LFSGARGTGKTTIARILAKRVNCLNPKDSEPCCECESCVSIQNGSSLDVQEIDAASNR
                     SIDNIRDLRENVKFQPMNAKRKVYIIDEVHMLTPEAFNALLKTLEEPPEHVLFVLATT
                     ELNKIPETILSRCQTFAFRKVPLNILQNHLKDICNREKIKAEDEALFWIARRGDGSVR
                     DSLSFLEQSITYTEGNVTTGKVKELIGALPLDLYLGLIQMLLDPKTKFPDLVEPVHRR
                     FDEGADLRRFIWDFLDLLRALSHVLHGVERPEFVGLPPTDIARLKEAFASADPARIQV
                     IFDAVHNIMTRAQTLRLENSYETRVLIEMELLRLHEKLGRPSLSFVLRRLDRLANAID
                     QGIPFSTEDEIQKTFLGTMVDPSTVPVLEQ"
     gene            6130..6684
                     /gene="pth"
                     /locus_tag="PKK76_07130"
     CDS             6130..6684
                     /gene="pth"
                     /locus_tag="PKK76_07130"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNL68281.1"
                     /translation="MADRFLIIGLGNPGKKYSKNRHNAGFLALDGVASAFGAGSFTKD
                     KKGEWALAQGPDKSEIYFYKPLEFMNLSGGGVKDFTKKHPVETARVVVLHDEIELPFG
                     EVRWKVGGGHKGHNGLRDIIARCGSPDFHRVRLGVGRPDHDDVAEYVLSDFMPEEFKS
                     FSLMTDRAKSLIEKWLVNPDKAPA"
     gene            complement(6687..>7155)
                     /locus_tag="PKK76_07135"
     CDS             complement(6687..>7155)
                     /locus_tag="PKK76_07135"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL68282.1"
                     /translation="YAIGNSLTEAEELAHRLTRHLARYADLEKASYIRLGLREILINA
                     IEHGNLEVSFDEKTETQMGGDYVSYLQERCSLSQFKGRMVRVAYSLNTKRVIYRIEDE
                     GKGFDFDALFARIHQSQPDSDLAHGRGILMTLNIFDTVRFVAPGNVVFLVKKF"
BASE COUNT         1746 a         1872 c         1732 g         1805 t
ORIGIN      
        1 acggtagcag gcaatatgcc ggtcgcatac ctgggtaaga agggaagctt tgcttccctg
       61 gtcgcacgac aaagatatcc ggacgcagag actgtctcac agccaacgat tggagatctg
      121 gttacctacg tgaaagaagg agcggaccga atgggtgttg tcccaatcga aaatagctcc
      181 ggtggaatca tttttgatac tgtggacgct tttgtggatg attcattcaa tcttttcatc
      241 ctggaagaaa cgcgcatccg cgtgcgatac gctttactgg gaaaatccgg gcaaccaatt
      301 cagaccattc tctcccacta tgcagctctg cgccactgcg cagcgtggat tcgctctaca
      361 tatccagcag tcaggacaca accagttgcc agcacgtcag aggccgccgc gatggccgcc
      421 gcgggatctc agcttgctgc gatttcaacg cgcgaatccg caaacgagta cggtctggac
      481 gttctgcatt ttccaatcat ggatagcgtt cccaacatta cgcaattctt tacgattggg
      541 catgcgccag taaactactc ggggtccgga ggaaacacaa gccttgtggc cactctggac
      601 aattcaccag gaagcctttt tcgattcctc aaaccctttc atgacctggg agtaaacctg
      661 aatagaattc aatcgcggac gattccggga gactcaagcg cgtgcaaatt ctttgtaggg
      721 ctcgagggag tggaatctga aacgaatgtg ggtcgcgcca tagaactggc tcgccctctc
      781 acggttgcgc tgcaactgct tggatcctat ccgagattga tggaccctta cgaatcttga
      841 cggtaggtca ctctgctttt tgcagaaaat cagaatcgtg cagatcttct tcaggcatag
      901 tatccgagta atccgccgga tcttcgcttc cgtctgaaat catggcttga gttgaatcca
      961 tcgaagaagc gggattggat tttccctgaa ctgtttctgt tcgatcatcc ggcgcagcgg
     1021 cattaccttt gccttttccg cggaattgaa acacagagcg cccgagcttg atctcatcgc
     1081 catcatgcaa gccgcgaggg cgaagtaatt tctttccatt caagaaaacg ctcgaaccac
     1141 ccattagatc gtataacaca taacggctgt ccacacgctt tacctttgcg tgaactttag
     1201 ccgctcctga atcctctacg cgaatgctgg cccatctgcc cgaaccaagg gccgtgtttt
     1261 caaggaagag atcatactct cgcgacattc catctttcat gcgaatcaga gaagcagcag
     1321 cataacgagg tgctccgcgg agtgcctcct gcaaagatcg atatgcgttc tcgcgcaaat
     1381 actgaacagc aggatgaggc atgtcctctg ctggaattcc ttctacaata tctactttct
     1441 ctgtttcgcc ttcgggaagc ttcacatgcg ccgttggcga atcggaagca gaaacaactg
     1501 ccgggttcgt ggcttttgcc gcgtcatgct tttgtttcag cacaaggatc agagctacaa
     1561 cagcggccac gaacaagaga aaagaaagca ctccgagcaa tacattgccc catggaatgg
     1621 aattgtacca cggtccctct gaatgtggga tcgatacgga tggagcgttg taagaagccg
     1681 tatcttcatc gatggtggtt cctcttttta cagatagaac aatgttgcgc ggccctgcac
     1741 cctccggtgc tttgtaagaa aggcgaaacg tttcatcttt gagaagtcga atggacctgg
     1801 aaagatttcg cattgtgtca ggcgtaagca tggtaatgag cttaccgccc gtgcgcatgg
     1861 atagtctctg gaagacgcgt tgattgcgcg gatgtccgta taacacagtg tagacgggaa
     1921 ctcgcagttt tgttcccagt tcagccagct caagacaatc ctgttcgttt agataactcg
     1981 cttcatcccg gccatctgta aacagtaaga cggccgcttt tgcgatttgc ttgcttgagc
     2041 tctccgtgtt ttgattgcga aggattcctt ctcgcgccgc gttgataccg ctgtgaagcg
     2101 cgtcaaaaat gcgagtcgtt ctgccgactc gtttcatttg cgctatttgt tcggaaatcg
     2161 ttttgccgcc aggaacaaat ccgctgatgt ttatacttcc gccattgatt tgaaagaggg
     2221 cagtgcgatc gctttctgtg agactggata agaaaacgcc agcaccgttc tgagcgcgac
     2281 gatagtccgc cgccggaatg gatccggtcg catcaaatac gactgccaga accagacgat
     2341 ccgcttcttt cctggaggca gtgatgctcg gcgtgatggg cgcgccatcg cgcagagtga
     2401 attgaggtgt tccctctcct tcgcccagtt ccgccagtat ttctacgttt ggaaaatgag
     2461 atgaatcgac ctcgagcacg cgcagagccg ggccagcatg aagaacaaat ggaatgagcc
     2521 atagcaggca gaagcgtgcc attgctttga ttatcggccg atcttacgga ctgccgatga
     2581 ttacatttcc ttccgtttcc ttcatcttct cgaggatttg tctgccttca gggctcgaaa
     2641 atcggtagta ttgccaggcg tgatgtccgc ccagaccaag aaatccaagc gccatgatcc
     2701 aggcagccag cctgcgagcg cctgtcattt ccccgagtac cccccggtgc aaaaagccaa
     2761 gggcaatcag aaccagatag atgcctatcc cggagtatat tatagaataa agtatatctc
     2821 gtatcattgt tttttccgtg ccaggtacgc ggcgcccatg ggacctgcca tgtctcccag
     2881 ttctgctagg cgaatctgtg gaagtggatt gatgcagcgc atggcaagct tgctttgaac
     2941 atgggggaga agcttcactc ccatggcctc catgattccg cctcccagga tcacaaggtc
     3001 cggattcagc atgttgactg cctgcgaaat cccgactgcc aggaacttgc aataatactc
     3061 gttcatgagt gcctgcgcga aaccgtcccc cttctcatac gcctttttca aactggacga
     3121 tttgatcgga gtctttcgca aattgtagtc aatcaggtcg cgaaggactg ttttcttttt
     3181 cattccaatg tatcgatcca ctctcttatt gagagcccat tttccaatgt aagactccag
     3241 acttcctctc aaaccactgt ccgtctttct gccaccatct ctgacaatga tgtgccccac
     3301 ttcaccggct cctccatcgc gaccaagaac cagttcgcca tttatggcaa tggctccgcc
     3361 aattcccgta ccgcagaaaa acgcaatggc aatcgacggt ttgtgttgca tgcgcgccag
     3421 ttctcccata agacctgcgt tcacatcgtt cacaagcacg gaacgaatgt ccctctgcac
     3481 aagaagtccg agatcctgca aagggtgtct attttcccag ttcaagttgg gagcaacacg
     3541 caccaggtct gtgtccttgg aaaccacgcc gggaacgccc aggccgattg tctcaattcg
     3601 aattcctttg ctttcgcaac tggcaatcag cgcatcgatc tggccacgca aacgaccaat
     3661 gacgtgattg tagccttctt ttgcttccgt atcgattttg ctttgcgtaa ctggatggta
     3721 agagtcgtca aaaacgactg agaaaatgtt tgaaccgccc agatctattc caaggtgccc
     3781 cgattgcata aatcggagta tgaagggagg tccacactgt caatcgattg atgacagttc
     3841 aatctccccg gcgataatcc tggcgctggc ggatgcttct gccaagtgtg actcgatccg
     3901 catactcaat ggcagcaccc gttggaacgc catgagagat tcgagtgatg ttcaaattgc
     3961 gaccggtgag cgcgttggcg atgtagtctg cagttacgtc tccctccagg gtgggattgg
     4021 ttgccagaag cacttcgttg atcctgtcat cggaggcaat cctggactca agctcggaaa
     4081 ttcgcaaatc tccaggacca atgccgtcta acggtgatat tgcccccatg agaacgtggt
     4141 agaggccttt gaactcaccc gtcttctcaa tcgcgtatac atctcccggt tcttccacaa
     4201 tacaaagctg gctgcggtcg cgcgtttcat ccctgcaaat cggacagaga tctgcgtctg
     4261 aaagaccacc gcaaagagaa cagaaacgga cttctttctt caatcgaatg agtgcttccg
     4321 ccagatcttc tgacttgtca gaatccgatc ttatcaaatg aaatgcaatg cgaagtgcac
     4381 tgcgcgaacc tatgcccggc aaagaagaaa gtaaacgcgc cgcattttct aacgacggaa
     4441 aaagcatcag gatccctggc gaaacatttc atcgagacca ggaatgccgc ctgtcagact
     4501 cttcatttcg tgcgccatgg cctcgcggga tttcttcaca gcttcgttgg tcgcggagat
     4561 gaccagctct tctagcattt ctttgtcttt cgggttgagc agattaggtt caatttgaat
     4621 tttaagtacg gtcccttctc cgttgacggt tacgcgaacc atgcccgctc ccgcttcccc
     4681 ggtgatttga agccgcgcca tttttgtctg cagttcttcc gcacgtgctt tcatctgccc
     4741 gaggttcttg aacatctcgc taaaattcat gaccatctca ttgctccaga acgggtacgg
     4801 tggaaggatc gaccatagtg cccaggaatg tcttttgaat ctcatcttct gttgagaaag
     4861 gaattccctg atcaattgca ttggccaggc gatccagtcg cctcagtaca aaggacagag
     4921 acggacggcc aagcttctca tgcaaacgaa gaagctccat ctcgatcagg acgcgagtct
     4981 catacgagtt ttcaaggcgc aaagtttgtg cgcgagtcat gatattgtga accgcatcaa
     5041 agatcacttg aatgcgagca ggatcagcgg aagcaaatgc ctctttcaat cgcgcaatgt
     5101 ctgtgggagg gaggcccaca aactctggtc tttccactcc gtgcaagacg tgcgaaagcg
     5161 cgcgaagtag atccagaaag tcccagatga atctccgcaa gtccgcacct tcatcaaacc
     5221 tgcgatgaac cggctccacc agatcaggaa atttggtttt aggatccaga agcatctgga
     5281 ttaaaccaag atagagatcc agaggcagcg ccccaatcag ctctttcacc ttaccggtag
     5341 ttacattgcc ttccgtgtac gtaatgcttt gttctaaaaa agaaagacta tcgcgaacag
     5401 atccatctcc acgtcttgca atccagaaaa gagcttcgtc ttccgctttg atcttctcgc
     5461 ggttgcatat gtctttcaaa tgattctgga gtatattcag aggtactttg cgaaatgcga
     5521 acgtctggca tctggataag atggtttctg gaatcttatt cagttccgtt gtagccaaaa
     5581 caaacaaaac gtgttcgggc ggttcctcaa gagttttcag cagagcgttg aacgcctccg
     5641 gtgtgagcat gtgtacttca tcaatgatgt agactttacg cttcgcgttc attggctgaa
     5701 actttacatt ctctctgaga tcgcgtatat tgtcgatgga gcgattggac gcggcatcaa
     5761 tttcctgaac atccagagat gatccgttct ggatggacac acaggattca cactcacagc
     5821 aaggttcgct gtctttagga ttcagacaat tcactctttt tgcaagtatc ctggcaatcg
     5881 tagtcttgcc ggtcccgcgc gcgccggaga acaaataggc ggaaccaatt ctgccatgat
     5941 ccgcagcgtt cttcaaagca gtaactgcga gcccctgctc tacaacttct ttgaatactc
     6001 tgggccgata cttgcgcgcg agcacaagat gcgcgacttc tgtcatgatg gatcaaggta
     6061 ccaatgctgt ttcgcagaac aacattattt tctgattgtg cccgatcgcg caaaacgcac
     6121 ggaggttgcg tggcagatcg cttcttaatc attggccttg gcaatcccgg gaagaaatat
     6181 tcaaaaaatc gacacaatgc cggatttctg gccctggatg gcgtagcatc cgcctttggc
     6241 gcaggctcgt ttacgaaaga taaaaaaggt gaatgggcac tcgcccaggg cccggacaag
     6301 tctgagattt acttttacaa acctcttgaa ttcatgaatc tttctggcgg tggcgtgaaa
     6361 gatttcacaa aaaagcatcc tgtggagact gccagagtgg ttgtactcca tgacgaaatc
     6421 gaacttccat ttggcgaagt acggtggaaa gtaggaggtg gccataaagg acacaatggt
     6481 ttgcgcgaca tcattgcgcg ttgcggaagt ccggactttc accgcgtaag acttggagtg
     6541 ggccgcccgg atcacgatga tgtagctgag tatgttctat ccgatttcat gccggaagag
     6601 ttcaaatcct tttctcttat gacagacaga gcaaagagcc tgattgaaaa gtggctggtc
     6661 aatccagaca aagcgccggc ctgaaactaa aatttcttaa ctaaaaacac gacattgcct
     6721 ggagcaacaa agcgaaccgt gtcaaaaata ttcagagtca tcaggattcc tctgccatgc
     6781 gccagatcgc tgtccggttg actctgatgg atcctcgcaa atagcgcgtc aaaatcaaat
     6841 cccttgccct catcctcaat gcgataaatg actcgtttgg tattcaagga ataggccact
     6901 ctcaccatgc gtcccttaaa ttgagaaaga ctgcatctct cctggaggta ggaaacatag
     6961 tctcccccca tttgcgtttc cgtcttctca tcgaatgata cttctagatt tccgtgctca
     7021 atcgcgttga tcagaatttc gcgcaggccc aggcgaatgt aggatgcctt ttccaggtct
     7081 gcgtatcgcg cgagatgtcg agtgagacga tgcgccagtt cttctgcctc cgtcaggcta
     7141 ttgccgatcg cataa
//
LOCUS       DAORFF010000135         7133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5312_length_7133_cov_3.410709, whole genome shotgun sequence.
ACCESSION   DAORFF010000135 DAORFF010000000
VERSION     DAORFF010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7133
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5312_length_7133_cov_3.410709"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PKK76_07140"
     CDS             complement(<1..474)
                     /locus_tag="PKK76_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNL68283.1"
                     /translation="MATAKNRSYFCAVDRYLNVRASLRRRLHAVVVALVLGPLAGTAF
                     ASEIMTLPSIRSGNAIRLLRDEISQNVKSSMRGQGLAVPIRFARYTVAKDDNFYRIVT
                     STHQDPDTLASLNHLAAAADLAEGDQILIPNARGIFREEGGPMSVYIPDAKMHFFA"
     gene            467..1057
                     /locus_tag="PKK76_07145"
     CDS             467..1057
                     /locus_tag="PKK76_07145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNL68284.1"
                     /translation="MAILSGLLVFVALASFFSLSTGWLPPGPMRPLLEKGVYGAIALA
                     SVLIYGKLLPVLFAPAPAIVARTVRTRVQIVAGITCGLALAGLLDLILAFFPEGHDPA
                     QSWQMIASGILASALEELLYRRGLQSYLSRWMPPVASVLVSSVCFAATHPMPGAFYVF
                     FCGLVFGVLYWRSGLLSAILAHGAYNFAILASAPWA"
     gene            1035..1757
                     /locus_tag="PKK76_07150"
     CDS             1035..1757
                     /locus_tag="PKK76_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68285.1"
                     /translation="MRRLRGRSGSFFLAAALCITSVQAETFRSFESAETPDALRWKLV
                     ALLNKVDSNNFQYEPETMGFAYLYQSIFTSPFNYRLYAGTVSEKQSMTMIRLEGRTGD
                     VNVFSRILEQEKLIKLDPLYTGEGKEIQYAALTPKYHWIGQPLNWVAPWLGVLHASYD
                     SPRLTTGQTVFRFSMYFGIDAVLVWAAGRNFFRDKFNAQKYAGNIAAALFLPRVIGSI
                     QSANLVRGHNRLVELKYTFPVQ"
     gene            1754..2224
                     /locus_tag="PKK76_07155"
     CDS             1754..2224
                     /locus_tag="PKK76_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNL68286.1"
                     /translation="MSLHKAIAVSLLLHAAFFAAAMIRIVYFPVIQAARGFAVEFMNS
                     GAQGQMNPGGQQGPGTHSTPGGGIKRGANTGTVSDLLNSITYPPLAEQMELEGTVTVE
                     TIVGKDGQTISAKVIKSSGHEILDRAASEGIMHWIFTDVASGVKTNIRVKFKLK"
     gene            2232..2897
                     /locus_tag="PKK76_07160"
     CDS             2232..2897
                     /locus_tag="PKK76_07160"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNL68287.1"
                     /translation="MTAPLKVIYEDNHLLVVDKPVGLLSQADSTGRPDLLTICKAYIK
                     EKYGKPGDVYLGLVQRLDLSVSGVIVFARTSKAAARLSEQIREREPIKIYQALVAGDP
                     PHEGHLEGFYYKDEEALMARKTERGKPGAKRASLSFRRLNSSDATHQALLEITLETGR
                     FHQIRVQLSDAGYPILGDEKYGGPRHKSGMALRCVSLTIQHPTRKELMTFKADGDWTR
                     AFA"
     gene            complement(2966..4147)
                     /locus_tag="PKK76_07165"
     CDS             complement(2966..4147)
                     /locus_tag="PKK76_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNL68288.1"
                     /translation="MAEPALMLSLTEEQLQLRDLVREVVKNEVVPVRMELDEKNEYPR
                     RVLEKFREAGIFQALFPEEYGGLGMGMMANIILSEEIAYGCLGVGTAFLATKLGSLPI
                     EVGATEEQKKKWLPQLASGEKVAAFGLTEPGAGSDVPALSTTAVKKGDRYILNGTKQW
                     ISGAGQADYYTVFALTAKDRGPRGMSCFLVEKGWKGFSFGKKEDKLGIRCSETRQLIF
                     EDCEVPAENIVGGRENNGFLHALKTLNLSRPAVAAQAVGTAQGAFDCAVQYAREREQF
                     GVKIATFQAIQHMLADMAIRIEGSRLLTYKSAALAEVGHKDTAKFSAMAKCYAADSAM
                     AVATDAIQIYGGYGYTKEYPVEKYFRDAKILQIYEGTSQIQKNEIAAGLIKEAASKVR
                     A"
     gene            complement(4264..5601)
                     /locus_tag="PKK76_07170"
     CDS             complement(4264..5601)
                     /locus_tag="PKK76_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNL68289.1"
                     /translation="MSDPLIHILIILGMILLNGFFAGSEIAIISLRKARVKMLLEQGD
                     PRAKTVQGLQSSPERFFATVQVGITVLGTLMSMYGAEQYADKLAPVLAGWLAPVPLLA
                     DYSLQISLGILVVIFSYLMLVLGDLAPKSLGHRYAEKFALAAAGPLLAFSRVFQVFIR
                     ISTWSANIVLWPFKDRTSFSETKLLPEEILQLLEEGVKTGSIAHSEHEIIENVLEFKD
                     TVAREVMIPRVDMKAVDVESSPEDAAVSLLDQFHSRLPVFSDNLDHIVGILHIKDLMK
                     ARARGDRYTLSTLARPAFFVPETMKIGDILKEMQKRKTHMAIVVDEYGGTSGLLTMED
                     ILEEIVGEIEEVTEQSAQGIESLPDGSYLVPGGCSIPDFNEYFDAMLEESDAYTSVAG
                     YLIHKAGRFPEVGETMTAESMQFELVRRVRQKLVQFRVTRVEPAPTAVDESKT"
     gene            5721..6473
                     /gene="map"
                     /locus_tag="PKK76_07175"
     CDS             5721..6473
                     /gene="map"
                     /locus_tag="PKK76_07175"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNL68290.1"
                     /translation="MIHIKSNTEIQKMKDAGKLAAEVLNETGRLVRPGVTTLELNDFA
                     HRLTLKRGAESAPLNYRGFPKSICTSINNVVCHGIPKKGETLKEGDIVNLDITVKLDG
                     YHGDTSRTFAVGKISDAAQKLMEDTEKSMWVGIEVVRPGARICDVGDSIDAFLTPKGY
                     GIVRDLCGHGIGRNFHEEPQVPHYKNNGIRSVMRPGMTFTVEPMVNRGSYEVFTDAHD
                     GWTVWTRDKSLSAQFEHTVLVTDDGYEVLTRE"
     gene            6470..6988
                     /locus_tag="PKK76_07180"
     CDS             6470..6988
                     /locus_tag="PKK76_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68291.1"
                     /translation="MNRLLLALLVLTSCVSGQNGSYPNFTSIELREYGIILHHPALWD
                     LNFDSKRKLPLVARGFTHDGRRASIEYRGIPQTQEDVDLYAEGWYKAMPSNFDSFEMV
                     DRKKISTEQGIVYHFEGTFREGDQRQRVVGRLRIRDGRVHAIYYLADDKDFSSFREIL
                     EDLDQSHRYFRP"
BASE COUNT         1771 a         1845 c         1809 g         1708 t
ORIGIN      
        1 tgcgaagaaa tgcatcttcg catccggaat atacacggac ataggtccgc cttcttctct
       61 gaaaatgccg cgggcgttgg gaatgaggat ctgatcgcct tctgccaggt ctgcggcggc
      121 cgcgagatga ttgagggatg cgagagtgtc cggatcctga tgcgtgctcg tcacgattct
      181 ataaaagttg tcatctttcg caacagtgta tcgcgcaaag cgaatcggca ctgccagccc
      241 ctgtcccctc atcgatgatt tcacattctg cgaaatctca tctcgcaaaa gacgaatcgc
      301 attcccgctg cgaatgcttg gaagagtcat gatttcagaa gcaaacgctg tgccggcaag
      361 cggaccaagc acgagggcga caactacagc atgaagcctt ctgcgaagag acgcgcgcac
      421 atttaaatat cgatctacgg cacagaagta tgatcgattt ttggccgtgg ccatcctctc
      481 tggactcctt gtttttgtgg ccctggcttc ttttttttcc ctgtccactg gctggcttcc
      541 tcccggtccg atgagaccac tcctggagaa gggcgtgtat ggtgccattg ctctggcatc
      601 cgttctcatc tacggtaagc tactcccagt cctatttgca ccggcaccgg ccatcgtcgc
      661 cagaaccgtc cgcacacggg tccagattgt agcaggcatc acatgcggcc tggccctcgc
      721 cggattgctg gatctgatcc ttgctttctt tcctgaggga catgatccag ctcaaagctg
      781 gcaaatgatt gcttccggaa ttcttgcgtc tgcactcgag gagctcttgt atagaagagg
      841 attgcagagt tacctttccc gatggatgcc cccggttgct tccgttctcg tttcttccgt
      901 ttgttttgcg gcaacgcatc caatgcctgg agcgttctac gtgtttttct gcgggctggt
      961 cttcggggtt ttatactggc gctccggtct gctttcggcg attctggcac acggtgctta
     1021 caacttcgcc atacttgcgt cggctccgtg ggcgtagcgg ctcattcttt ctggctgccg
     1081 cactctgcat cacatccgtg caggcggaaa catttcgatc ctttgaatcc gccgaaaccc
     1141 cggacgcgtt gcgctggaaa ctcgtggcgc ttctcaacaa agtagatagc aacaacttcc
     1201 aatacgaacc ggagacaatg ggctttgcct atctctacca gagtatattc acttcgccat
     1261 tcaattacag actctacgcg gggaccgtaa gcgaaaaaca atccatgacc atgattcgcc
     1321 tggaagggcg cacaggtgac gtgaacgttt tctcgcgcat actcgaacag gaaaagttaa
     1381 tcaaactgga ccctctctat acaggagaag gaaaggaaat ccagtacgca gccctcactc
     1441 caaaatacca ctggataggc cagcctctca actgggtggc accgtggctg ggcgtgcttc
     1501 acgcgtctta tgattctccg cgcctgacaa cgggacaaac agtgtttcga ttctccatgt
     1561 acttcggaat cgacgcagtg ctcgtatggg cggcaggacg aaactttttc agggacaaat
     1621 tcaacgcaca aaagtatgca gggaacatcg cagccgctct ctttctgcca cgagtcatag
     1681 gctccatcca atccgcaaat ctagtgcgcg gacacaaccg tctggtggaa ctgaagtaca
     1741 cttttcccgt acagtgagcc tgcataaagc catcgcagtc tccttactgt tgcatgctgc
     1801 atttttcgca gcggccatga ttcgcatcgt atacttcccg gtgattcagg ctgcccgcgg
     1861 ttttgcagtc gaattcatga acagcggagc ccagggacaa atgaacccgg gcggacagca
     1921 gggtcctggg acacattcga ctcccggggg cgggatcaaa cgaggcgcaa acacaggaac
     1981 tgtttcagat ctactcaata gcatcaccta tccaccgtta gctgaacaga tggaactgga
     2041 aggaaccgta accgtggaga caattgttgg caaagacggg cagactatct ctgcaaaggt
     2101 gatcaagtcg agcggacacg aaatactcga ccgcgcggct tcagagggaa tcatgcactg
     2161 gatcttcacg gacgttgcaa gcggtgtcaa aacgaacatt cgcgtaaaat tcaaactcaa
     2221 atgaagcgaa catgactgct ccactcaaag tcatctacga agacaatcac ctcctggtcg
     2281 tagataaacc ggtagggcta ctttcgcaag cagacagcac tggaaggccg gatctgctga
     2341 cgatctgcaa ggcatacatc aaagagaaat atggcaaacc cggggacgtt tatctggggc
     2401 tggtacagag gctggatctc agtgtcagcg gtgtcatcgt atttgcacgc acgtcaaaag
     2461 cagccgctcg cctgagcgaa caaatcagag aacgcgaacc aatcaagatt tatcaggcac
     2521 tggtcgcagg agatccgccc cacgaaggcc atctcgaagg tttctattat aaagacgaag
     2581 aagcgctcat ggcaagaaag acagaacgcg gaaaaccggg ggcgaaacgc gcctctttga
     2641 gttttcgaag attgaattca agtgacgcga ctcatcaagc tcttctcgaa atcactctgg
     2701 aaaccggtcg ctttcatcaa attcgagttc aattgagcga tgctggatat cctattctgg
     2761 gtgatgagaa atatggaggc cctcgacaca agtcggggat ggcgctacga tgcgtttctc
     2821 tgacgattca acatcctacg cgaaaagagc tcatgacatt caaagcggac ggagactgga
     2881 ccagggcatt cgcgtagaat ccggacaaaa aaaaggaacc ctctcgggtt ccttctctcg
     2941 aaaatcgcct gtggcgattc tacaatcagg cgcgaacttt ggaagccgct tccttgatta
     3001 gacctgctgc gatttcgttc ttctggatct ggcttgtgcc ttcgtagatc tgaagaatct
     3061 tcgcgtcgcg gaaatacttt tcaactggat actctttcgt gtatccgtag ccgccataaa
     3121 tctgaattgc atctgtcgca acagccatgg cagaatcagc agcgtagcat tttgccatcg
     3181 cagagaattt agcagtgtct ttgtgtccaa cttcagcaag tgctgctgat ttgtaagtaa
     3241 gcagacgaga tccttcaatg cgaatcgcca tgtcagcaag catgtgctgg atagcctgaa
     3301 atgttgcgat cttcacgcca aactgttcgc gttcacgggc atactgaacc gcacaatcga
     3361 atgcgccttg agcagttcct acagcctgag ctgccacagc cggacgagag aggttcagag
     3421 tcttgagcgc gtgcaggaag ccgttgtttt cgcgtccacc tacgatgttc tccgctggaa
     3481 cttcgcagtc ttcgaagatc aactgacggg tttcagagca acgaatgccg agtttatctt
     3541 ctttctttcc gaaagagaaa cctttccatc ccttctctac aaggaagcat gacataccgc
     3601 gaggaccgcg atcttttgca gtgagggcga atacagtgta gtaatccgcc tggcctgcgc
     3661 cagaaatcca ctgcttggtt ccgttcagga tgtaacgatc tcctttcttc acagccgttg
     3721 tggaaagtgc tggaacgtct gaacccgcac ccggttctgt aagaccgaac gcagctactt
     3781 tttccccgct tgcaagttgc ggaagccact tcttcttttg ttcttctgtc gcgcccactt
     3841 cgatcgggag agatccaagc ttggttgcga gaaacgcagt gcccactccg aggcatccgt
     3901 atgctatttc ttcagacagg atgatgttgg ccatcatacc cattccaagt ccaccgtact
     3961 cttctggaaa cagtgcctgg aaaatgcccg cttcacggaa cttctcaagt acgcgacgcg
     4021 gatattcatt cttttcatct agttccatgc gcacaggaac tacttcattt ttcacaactt
     4081 cacgcactag atcgcgaagc tggagttgtt cttcagttaa ggaaagcatc aatgctggtt
     4141 cagccatacg accttttttt taggtttggc atttgaggct agaagatttt ggaacgagtc
     4201 gtcgcggaca atcgcgtccg gaatgctcgt aaatgagaca taatctaaag tgctttcgga
     4261 gatttaagtc ttactttcgt ccaccgcagt tggcgcgggc tccactctgg tgacgcgaaa
     4321 ttgaaccagt ttctgacgca ctcgtctgac gagctcaaac tgcatcgatt ccgctgtcat
     4381 ggtttctccg acttccggaa agcggcccgc tttgtgaatc agataaccgg ccacgctggt
     4441 gtaggcgtcg gactcttcga gcattgcatc aaagtattcg ttgaaatcag ggatggaaca
     4501 acctccaggg accagatagc tgccgtctgg aagggattca attccctggg ccgattgctc
     4561 cgttacttcc tcaatttccc caactatctc ctccaggatg tcctccatgg taagaaggcc
     4621 ggaagttcca ccgtattcgt ccacgacgat ggccatatga gtctttctct tctgcatctc
     4681 ttttagaatg tcgcctatct tcatagtctc gggcacgaag aacgcaggac gagcgagagt
     4741 actgagtgtg tagcgatctc ccctggcgcg tgccttcatg agatccttga tgtgcagaat
     4801 cccgactatg tgatcgaggt tatccgagaa aacaggaagg cggctgtgaa attgatccag
     4861 aagggacacg gccgcatctt ccggagagga ctctacgtcc actgccttca tatctacgcg
     4921 tggaatcatt acttccctgg cgactgtgtc cttgaactcg agtacgttct ctatgatttc
     4981 gtgttcgctg tgcgcaatgc taccagtctt tactccttct tcgagaagtt gcaggatttc
     5041 ctccggcaag agcttggttt ctgagaagct cgttctgtct ttaaatggcc agagtactat
     5101 gttcgcagac caggtagata tgcgaatgaa gacctggaag acacgcgaga aagccaggag
     5161 cgggccggct gccgccagcg cgaacttctc cgcatacctg tgccccagag atttgggcgc
     5221 caggtcaccg agaaccagca tcagataaga gaatataaca acgagtatgc caaggctgat
     5281 ctgtagagag taatcagcta acaggggcac aggtgccagc cagccggcca gaactggcgc
     5341 cagcttatcc gcatactgtt ctgcgccata catggacatc aaagtcccca gtacagtgat
     5401 acccacctgg acggtggcaa agaaacgctc ggggctggac tggaggccct ggactgtttt
     5461 tgcgcgcgga tctccctgct ccagaagcat cttgacccga gcctttcgca gcgatataat
     5521 ggctatctcg ctgccggcga aaaatccgtt cagcaggatc attcctaaaa tgattagtat
     5581 gtgtatgagc ggatcagaca ttcaaaagag gacaaagaat catcgaactt tgccctcgaa
     5641 cgggctcggc acgatcaggt gcgcaagtta atggatgccg cacggcctgc cagtcttttt
     5701 ttttaacaat gtagaaccaa atgatacaca taaaaagcaa cactgaaatc cagaaaatga
     5761 aagatgcggg caaactggcc gcagaagtac tcaatgagac aggccgcctg gttcggccag
     5821 gggtcaccac gctggaactg aacgattttg ctcatagatt gaccttgaaa cgcggggcgg
     5881 aaagtgcccc cctgaactac cgcggtttcc cgaagagcat ttgcacttct atcaacaacg
     5941 tggtgtgcca cggcattcca aagaaaggag aaaccctcaa agagggggac atcgtaaacc
     6001 tggatatcac agtgaaactg gatggatacc acggcgacac atctagaacc ttcgcggttg
     6061 gtaagatcag tgatgcggcg cagaagctca tggaagacac cgagaagtct atgtgggtcg
     6121 gcattgaagt agtcagacca ggtgcgcgta tctgcgatgt cggagactcc attgatgcgt
     6181 ttctgactcc gaaaggatat ggaatcgttc gtgatctgtg cggtcacgga attggcagga
     6241 actttcacga agagccccag gtcccacatt acaagaacaa tgggatccga tccgttatgc
     6301 ggcctggaat gacctttaca gtagaaccaa tggtaaatcg cgggagttat gaagtattta
     6361 cagatgctca cgacggatgg accgtctgga ccagagataa atctttatcg gcacagttcg
     6421 agcacacggt cctcgtaacc gatgatggat atgaggtact cactcgcgaa tgaatcgttt
     6481 gttgcttgca ctgctggtgc tgacatcctg tgtcagcggc cagaacggct cgtatccaaa
     6541 tttcacgtcg atcgagttgc gtgaatacgg aatcatcctg caccaccctg ccctctggga
     6601 tttgaatttt gacagcaagc gaaagctacc tctggtcgcg aggggattca ctcacgatgg
     6661 aagacgggcg agcattgagt atcgcgggat tccgcaaacg caagaagatg tagatttata
     6721 tgcagaaggt tggtacaaag ccatgccttc taattttgat tcttttgaaa tggtagatag
     6781 aaagaagatc agcacggaac agggaatcgt atatcatttt gaaggaacat ttcgcgaagg
     6841 ggatcaaaga caaagagtag tcgggcgatt gcgcatccgc gatggaagag tgcatgccat
     6901 ctattatctt gcagacgaca aagacttttc ttctttccgc gaaatccttg aagatctgga
     6961 tcaatcacat cgatactttc gcccttgatt cttctttcca ctcgcgcgaa ttacaaaatc
     7021 gattgacaaa tcattaaacg gttgataagg acgagtcgga atccctgatg ggtcttcggc
     7081 gcctatagct caatggatag agcgcaggtc tccggaacct gaaatcgggg ttc
//
LOCUS       DAORFF010000136         7102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5356_length_7102_cov_3.185469, whole genome shotgun sequence.
ACCESSION   DAORFF010000136 DAORFF010000000
VERSION     DAORFF010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5356_length_7102_cov_3.185469"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PKK76_07185"
     CDS             complement(<1..1012)
                     /locus_tag="PKK76_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68292.1"
                     /translation="MNQILFFSFLIFLFAGIGMSFVWLRLSARESRLLTLLLGAASAV
                     PFSTIAYIYGLSTNVIFWILCLWAGCGFILRLYSWRPARFFRRIRKSFVFHAIWIACA
                     AILLAPRLAGGSQFAVYQANRWDTFGYLESASSYSRYSFDFVEHATLADQLADPLLVQ
                     GQIQMFVRPSIHMFYGVVSHFLPGEFWDLHYTFLLSFVLLSTFACAFFLSYLFRVKSI
                     YGMIAGAAFSLGFWGQYSLDINAWSQVASTGVLVTLTALLLRLRMRKLNLRTAILISL
                     FSAGSLYLYPEAFVVVGACSALSAVVSALWLRNPRTLILPAVGGTMGVAAGFLFFHGT
                     IQF"
     gene            complement(1009..1416)
                     /locus_tag="PKK76_07190"
     CDS             complement(1009..1416)
                     /locus_tag="PKK76_07190"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNL68293.1"
                     /translation="MLRAFHNTKLRFLFAGGSTAVIYFGLLYLLHDMMGQSSNLSVGT
                     AYFVSTAYHFLCNKFFVHRDDSLDTAHLQLGKYLALIAVNYAITWVVTNGILVMGGNV
                     YLGVCASVLITLVFTYVVMRRAIFRRGSEPREQ"
     gene            complement(1397..2116)
                     /locus_tag="PKK76_07195"
     CDS             complement(1397..2116)
                     /locus_tag="PKK76_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNL68294.1"
                     /translation="MTPSVAILAGGLATRIRPITDKIPKALIPIRGRPFVDYQLELLA
                     GMNLSRVVLCTGYKGEMIEDHVGDGSAFGVSVQYSHDGETLLGTGGALKRALPLLGDR
                     FIVIYGDSYLDADYRPIVEFFDTQSSASHRPQALMTVFRNEGLFDRSNVVFRGGRIIV
                     YDKKQDHPEMNYIDWGLGILHAGVFAPFSERSVFDLAELYGHVLSEGALLGYEVKQRF
                     FEIGSVSGIEEFERHIASRLP"
     gene            complement(2113..3093)
                     /locus_tag="PKK76_07200"
     CDS             complement(2113..3093)
                     /locus_tag="PKK76_07200"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68295.1"
                     /translation="MIIVRSPLRISLGGGGTDLASYYLDHGGFLIAAAIDRYVYITLH
                     ETFIDEIIVKYSKLERVSSADQIEHPIIRECLKLLDIKRTNIELTSMADIPAGTGLGS
                     SGSFTTALLKTLHTYNKNIITTHELAEQACHVEIDRLKEPIGKQDQYIAAYGGITCFE
                     FRKDHSVKASALDLSEETRLDLEDNLLLFFTGYSRSASSILKEQDDKSKQNNADMIQN
                     LHFIKDIGKQSKTALETGNLHEFARLMDVHWQEKKKRSSGMSNQKINEWYDFAMQNGA
                     LGGKLIGAGGGGFLMFYTENKTKLRHAMMSLGLVEVRFRFDFEGTKVVIS"
     gene            complement(3090..4076)
                     /locus_tag="PKK76_07205"
     CDS             complement(3090..4076)
                     /locus_tag="PKK76_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNL68296.1"
                     /translation="MKIGIVGCGLIGRKRAASVDADDRIVAAYDTNATVLGKFCADFK
                     CEASPSAAQLAARADVDAVIVSAVHSALAEISREVISAGKHLLVEKPAARNAQELRPV
                     VALLESLPQEKRVRAKCGFNHRFHPAMLKAKELLESNDCGELMFVRGRYGHGGRPGYD
                     REWRADPEISGGGELLDQGMHLIDLSRWFLGDLPRVQGIARTFFWDMKVDDNAFMLLE
                     TEKGQVAQLHVSWTEWKNLFSFEIYAKRAKLHIEGLGGSYGLERIAFYPMKPEMGPPD
                     SLIYEFPGADLSWKREWKNFKASIEKGEPLLGSLHDALAALEIVDTIYRRKS"
     gene            complement(4073..4942)
                     /locus_tag="PKK76_07210"
     CDS             complement(4073..4942)
                     /locus_tag="PKK76_07210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNL68297.1"
                     /translation="MKLAHTTAIITGASQGLGYAIAEAFADAGANVVITARSLDGIAK
                     ASQSLASRSRPGRIVHHAADVTDESAMKRVVESAIAEFGRIDTLVNNAGVYGPIGPLE
                     ETDLAEWKYAFDVNFYGTLIPCRLLLDHFKKNRAGRIINLSGGGATKGMPSFSCYAAA
                     KAAVVRLTETLALEAGEFGITVNAVAPGALNTRLLDEVLQAGPEKTGRQFFEASIKQR
                     DSGGSSLQNAAELCVFLAGQEARSINGRLISAVWDDWKNLPGRAELLAKSDVYMLRRI
                     TAKERGFDWDDSK"
     gene            complement(4939..6216)
                     /locus_tag="PKK76_07215"
     CDS             complement(4939..6216)
                     /locus_tag="PKK76_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNL68298.1"
                     /translation="MNISVFGLWHLGCVTAACLAADHSVTGIDDDSSLIESLKQGRAP
                     VSEKGLDELIQSHNGKTLHFTTDLSSCAGADLIWITFDTPVDENDNADVEFVVEKMRR
                     VIGFARSGAVVAVSSQLPVGTVRRMMKEASDAKKNLHFACIPENLRLGAAIECFVRAD
                     RYVVGTDSDSAREVLTQVLSHYTANVIHVSPESAEMSKHALNGFLALSVVYANEIAAI
                     CERTGADASEVERCLKADVRIGKGAYLSPGPAFSGGTLARDASFLGRISKEKRLGLKV
                     VPSILAANEEHKFWPVRALKPLRDSGAARVCVLGLTYKPCTDTLRRSLPLEICARLRE
                     DGWRVCAYDPAVKHLPDSHAFIDLKQSAAEAMRDADALILLTAWPEFKEMTLQSIPPE
                     KSPAIVDPAGVWKGWFSGGNARYFSIGRSRGAF"
     gene            complement(6247..>7102)
                     /locus_tag="PKK76_07220"
     CDS             complement(6247..>7102)
                     /locus_tag="PKK76_07220"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNL68299.1"
                     /translation="STGNAQFLAKAEKQQGFALVKGDLLDLAALRTAMAGHDTVFHLS
                     ANADVRMGTAHPSRDIEQNTQGTFHVLEAMRENGIKRIMFSSTGSVYGEAAVIPTPED
                     APFPVQTSLYGASKLAGEGLIAAYCEGFGFQSWIFRFVSILGERYTHGHVFDFYKQLK
                     ENPSQLRVLGNGKQRKSYLYIGDCVQAILHAVEHAADRVNIFNLGTAEYCEVLDSVRW
                     ICDELKCSPEITLTGGDRGWIGDNPFIFLETAKIRGTGWAPKLTIEQAVRRTVQFLKE
                     NEWVFAAR"
BASE COUNT         1818 a         1957 c         1774 g         1553 t
ORIGIN      
        1 tgaattgaat cgttccgtga aagaaaagaa atccagccgc aactcccatg gttcctccca
       61 ctgccggaag gatcaacgtg cgcggattgc gaagccagag agcggagaca actgcagaga
      121 gcgcagaaca tgcacccact actacaaaag cctccggata aagataaaga gatcccgccg
      181 aaaatagaga gatcagtatt gccgtgcgca gattcaattt tcgcatgcga agacgaagaa
      241 gaagagctgt cagagtcaca agcacgcccg ttgacgcaac ctgactccag gcgttgatat
      301 ccagagaata ttgtccccag aaacccagtg aaaatgcagc gccggcgatc atgccgtaga
      361 ttgacttcac gcgaaacaaa taggaaagaa agaatgcgca ggcaaaagta gaaagcaaaa
      421 caaatgaaag cagaaaagta tagtgcagat cccagaattc tcccggaaga aagtgggaca
      481 ccacgccgta gaacatatgg atggacggac gcacaaacat ctggatctgt ccctggacca
      541 gcaacggatc ggccagctga tcggctaacg ttgcatgttc aacgaaatca aaagaatacc
      601 tggaataaga agaagcagat tccagatagc caaaggtgtc ccagcgattt gcctggtaga
      661 ctgcaaattg actgcctccc gcaagtctcg gtgcaagcaa gatcgcagcg catgcgatcc
      721 agattgcatg aaaaacaaat gatttgcgaa tccttctaaa gaaacgggcc ggcctccagg
      781 aatacaatct cagaatgaaa ccgcagcctg cccacaggca tagaatccag aatatcacat
      841 tcgtgcttag cccgtatata taggcgatcg tagaaaaagg aacggcagat gcagcgccca
      901 gaagcagcgt cagaaggcgc gattctctgg cagacaggcg aagccagacg aaggacatgc
      961 ctatccccgc aaaaagaaaa ataagaaagc taaaaaagag aatctgattc attgttctct
     1021 gggctcagaa ccccggcgaa agatggcccg gcgcatcaca acgtatgtga acacaagcgt
     1081 aatcaacacg gatgcgcaaa cacccagata tacattcccg cccattacaa gaattccgtt
     1141 ggtcacaacc caggtaattg cgtagtttac ggcgatcaag gcaaggtatt taccaagctg
     1201 caggtgcgcc gtatccagag aatcatctct gtgcacaaag aacttgttgc aaagaaaatg
     1261 atacgcagtc gaaacaaagt atgctgtccc gacggacagg ttgctgctct gccccatcat
     1321 gtcatgaagc agatacaaca gtccaaagta aatgacggcc gtacttccgc ctgcgaagag
     1381 gaaacgcagt ttggtattat ggaaggcgcg aagcaatatg tctttcaaat tcctcaatcc
     1441 cggaaacaga accaatttcg aaaaagcgct gtttgacttc ataacccaga agcgcacctt
     1501 ctgaaagaac gtgtccgtac agctccgcca ggtcaaacac agagcgctcg ctgaatggtg
     1561 caaaaacgcc ggcgtgcaaa ataccaagcc cccagtcgat gtagttcatc tccggatgat
     1621 cttgcttttt atcataaacg atgattctgc cgccgcgaaa cacaacattg ctccgatcaa
     1681 acaagccttc attgcgaaat acagtcatga gagcctgggg ccgatgcgaa gcagatgatt
     1741 gcgtgtcaaa aaattcaaca atcggacgat agtcggcatc gaggtaggaa tcgccgtaaa
     1801 tgacaatgaa tcgatctcca agaagaggaa gtgcacgttt gagcgcgccg ccagtcccca
     1861 gaagtgtttc tccgtcgtga ctgtattgaa cagagacacc aaaagcggaa ccatctccaa
     1921 cgtgatcctc gatcatttcc cctttgtagc ctgtgcacag cacgacgcgg cttagattca
     1981 tgccggcaag cagttcaagc tggtaatcta caaacggacg tccgcgaatc gggatcagtg
     2041 cttttggaat tttgtccgta attgggcgaa tgcgagtggc cagaccgcca gcaagaatgg
     2101 caaccgaagg ggtcacgaaa taactacctt cgttccttca aaatcaaaac ggaagcgcac
     2161 ttccacaagc ccgagactca tcatcgcatg acggagttta gttttgtttt cagtgtagaa
     2221 catgagaaac ccgccgccgc ccgcgccgat cagcttaccg ccaagtgcac cgttctgcat
     2281 ggcgaagtca taccactcat tgatcttctg attgctcatg ccgcttgatc gtttcttttt
     2341 ttcctgccag tgtacgtcca tgaggcgtgc gaactcgtgc aggttgcctg tctcaagcgc
     2401 agtcttgctc tgctttccaa tgtcttttat gaaatgcaga ttctgaatca tatccgcatt
     2461 gttctgtttg ctcttgtcgt cctgctcctt gagaatcgaa gaagcagacc tggaataacc
     2521 ggtaaagaaa agaagtaaat tatcttcaag atcaagtcgc gtctcttccg aaagatccag
     2581 cgccgatgcc tttacggaat gatccttgcg aaattcaaaa caggtgatgc cgccgtaagc
     2641 ggcaatgtac tggtcctgct ttccgatcgg ctctttgagt cggtcgatct ccacgtgaca
     2701 ggcctgttcg gcgagttcgt gtgttgtaat gatgttcttg ttgtaggtgt gcagagtctt
     2761 cagaagcgcc gtcgtgaagc ttccggagga acccagtccc gtgccggccg gtatgtctgc
     2821 catactcgta agctctatgt tcgtgcgctt gatgtctaga agcttgagac attcgcggat
     2881 gatcggatgc tcgatctggt ctgctgagga tactcgttcc agctttgagt acttcacaat
     2941 gatttcatca atgaaagtct cgtgaagagt gatgtagacg tagcggtcaa tcgcggcggc
     3001 aatcagaaag ccgccgtggt ccagatagta agaagcgagg tctgttcctc ctcctccgag
     3061 tgaaatccgc aacgggctgc gaacaatgat catgattttc tccgatagat tgtatctacg
     3121 atttcaagtg ctgcaagcgc atcgtgcagc gagcccaaca gaggttcgcc cttttcaatc
     3181 gacgccttga agtttttcca ttcgcgcttc cacgacagat ccgcgccagg aaactcatag
     3241 atgagagaat cgggcggccc catctccggc ttcataggat aaaatgcgat gcgctcaaga
     3301 ccgtaactgc ctccgagacc ttcgatatga agctttgcac gcttggcata aatttcaaat
     3361 gagaacagat tcttccattc tgtccagctg acgtgaagct gcgccacctg ccctttttct
     3421 gtttcaagaa gcatgaacgc attgtcgtcg actttcatgt cccagaagaa agtgcgcgca
     3481 atcccttgca cccgcggaag atcccccagg aaccagcgcg aaaggtcaat caaatgcatc
     3541 ccttgatcga gcagctcgcc gccgcctgaa atctcgggat cagcacgcca ttcccgatca
     3601 tagccaggcc ggccgccgtg accatagcga ccccgcacaa acatcaattc gccgcaatcg
     3661 tttgactcaa gaagctcctt tgccttgagc atcgcaggat gaaaacgatg attgaatccg
     3721 catttcgcgc gtactctttt ctcttgagga agtgactcaa gcagcgcaac aacagggcga
     3781 agctcctggg cattgcgcgc ggcaggtttt tccacaagga gatgctttcc cgcagaaatg
     3841 acctcgcgcg aaatctcggc cagggccgag tgaacggcgc ttacaatcac agcatcaacg
     3901 tccgcccgcg cggcgagctg tgctgcactc ggagaagctt cacacttgaa gtctgcgcaa
     3961 aacttaccga gcactgtcgc gttcgtatcg tatgcagcaa cgatccggtc atccgcgtcc
     4021 acggacgcgg ctcgcttgcg tccaatgaga ccgcaaccaa caatgccgat cttcattttg
     4081 aatcgtccca gtcaaatccg cgctcttttg ccgtgatccg gcgcagcatg tatacgtccg
     4141 atttcgcaag cagctccgcg cgccctggaa gattcttcca gtcgtcccat acagcgctga
     4201 tcaagcggcc gttgatcgac ctggcttcct gtccggccag aaagacgcag agctctgctg
     4261 cgttctgaag cgaagaaccg ccgctgtccc gttgtttgat ggatgcctcg aaaaactgtc
     4321 tgccggtctt ttcgggtccg gcctgcagca cttcgtctag cagtctagta ttgagcgcac
     4381 ctggcgcaac tgcgttgaca gtgatcccga attctcccgc ttccagtgca agcgtctccg
     4441 tcaggcggac cactgcggcc ttggccgccg cataacaact aaaagatggc attcccttcg
     4501 tagcgccgcc gcccgagagg ttgatgatcc gcccggcgcg attcttcttg aaatgatcca
     4561 gcagcagacg gcacggaatg agagtcccat agaagtttac atcaaacgca tacttccact
     4621 ccgcgagatc cgtctcttcg agcggcccga ttggtccgta tacgcctgcg ttgtttacca
     4681 gagtatcgat ccggccgaac tctgcgatgg ctgactccac cactctcttc atggcagatt
     4741 catccgtcac atccgctgca tggtggacga ttcggcccgg cctggaacgc gaagcgagac
     4801 tctgcgatgc tttcgcaatt ccatccaggc tgcgcgctgt aatgacaacg ttcgctcccg
     4861 catcggcgaa ggcctccgca atcgcgtatc cgagaccctg gcttgcgcct gtgatgattg
     4921 cagtagtatg agccagtttc aaaatgcccc cctcgatcga ccgatggaaa aataccgtgc
     4981 atttccgcct gaaaaccatc ccttccatac acccgcagga tcgacaatgg ccggagactt
     5041 ctcaggcgga atggattgca gagtcatttc tttgaactca ggccaggcag taagaagaat
     5101 caacgcatcc gcatcgcgca ttgcttctgc ggcgctttgc ttgaggtcta tgaatgcgtg
     5161 cgaatcagga agatgcttca ccgctggatc ataagcgcac acacgccatc cgtcttcgcg
     5221 cagtctcgcg catatctcca gaggcagaga tcggcgcaga gtgtccgtgc agggcttgta
     5281 tgtcagaccc agaacgcata ctctcgctgc tcctgaatca cgcagcggtt tgagagcgcg
     5341 aacaggccag aacttatgct cttcgttggc agcaagaatc gacggaacca ccttcaaacc
     5401 cagtctcttc tctttcgaaa tgcgcccgag gaaggaagca tcccgcgcca gagtcccgcc
     5461 tgagaaagcg gggcccgggg acagataggc gcccttgccg attcttacgt ctgctttgag
     5521 gcagcgctcg acttcggatg catctgcgcc cgtgcgttca caaatcgcag cgatctcgtt
     5581 ggcgtagaca acagaaagcg caagaaagcc gttgagggca tgcttactca tttccgctga
     5641 ttctggagaa acgtgaatca cattcgcagt gtaatgcgaa agaacctgcg tcagcacttc
     5701 gcgcgcggaa tctgaatctg taccgaccac gtatcggtcc gcgcgtacaa agcattcaat
     5761 tgcagctccc agacgcagat tttcagggat gcaggcaaaa tgcagattct tcttcgcatc
     5821 tgaagcttcc ttcatcatgc gtctgaccgt tccaaccgga agctgggatg agactgcgac
     5881 cacagcaccg ctgcgtgcaa agccaatcac gcggcgcatc ttctccacaa cgaactctac
     5941 atctgcattg tcgttctcgt cgactggagt atcgaatgtg atccagatca aatccgcgcc
     6001 ggcgcacgac gaaagatctg tagtaaagtg cagggtcttc ccattgtgcg attgaatcaa
     6061 ctcgtcgagc cccttctcag aaacgggcgc ccgcccctgt ttcagtgatt cgatgagaga
     6121 agagtcatcg tctattccag ttacgctgtg gtcagccgca aggcaggccg ccgtcacaca
     6181 gccaaggtgc cacaatccaa atacgctgat attcatgcct gtaattcctg ccggaccgct
     6241 ggagtttcag cgagccgcga agacccattc gttttctttg agaaactgca ctgtgcggcg
     6301 cacggcctgt tcgatcgtga gttttggcgc ccagccggtt ccccggatct tcgctgtttc
     6361 aagaaaaatg aaaggattgt ctccaatcca cccgcggtct ccgcccgtca gagtgatctc
     6421 gggcgaacac ttcaactcat cgcagatcca gcggaccgaa tcgagcactt cgcagtactc
     6481 agcagtgcct agattgaaaa tgttcacgcg gtctgccgcg tgttcaacag catggagaat
     6541 cgcctggacg cagtcaccaa tgtatagata tgactttctc tgtttgccgt tgcccagaac
     6601 gcggagctga gatggatttt ctttcaattg tttatagaaa tcaaacacat gtccgtgagt
     6661 atagcgctct ccgaggatag atacaaatcg aaagatccat gattgaaagc cgaagccttc
     6721 gcaataggca gcaatgagac cctcgcctgc aagcttggaa gcaccataga gcgaagtctg
     6781 aactggaaag ggcgcgtctt caggcgtggg aatcacagcc gcctcaccgt agactgagcc
     6841 ggtcgaggag aacatgattc gcttgattcc gttttcgcgc atagcttcga gaacgtggaa
     6901 ggtgccctga gtattttgct ctatatcacg tgaaggatgc gctgttccca tgcgaacatc
     6961 cgcattcgcg gaaagatgaa acacagtgtc gtgaccggcc atcgcagtgc gaagcgcggc
     7021 taaatcgaga agatcgcctt ttacaagggc aaagccctgc tgcttttccg ccttcgccag
     7081 gaactgcgca tttcccgttg aa
//
LOCUS       DAORFF010000137         7086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5377_length_7086_cov_2.975537, whole genome shotgun sequence.
ACCESSION   DAORFF010000137 DAORFF010000000
VERSION     DAORFF010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5377_length_7086_cov_2.975537"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="PKK76_07225"
     CDS             <1..1240
                     /locus_tag="PKK76_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LIC12162 family protein"
                     /protein_id="HNL68300.1"
                     /translation="GESYRLPGHFGPPPRMSLARRLRRVPGLIRQRLVVRGTDSFIFN
                     TYMQAEEEVLLQRGLGQTPRRWHSHHFFGPSVDPDLRRELSASFSGDSSGLMGCMRAL
                     LFELIPVCYLEGYRDLVKKSESMPWPDRPRLIFTSGNYDTDEIFKCWTASRVERGSRY
                     MVGQHGNNYGTHRYMAYRTTDQLTSDRFLTWGWRDSLPSHTPAFILRTSGFPELDPAG
                     GLLLAEDGLPNRVYTWDIFPDYTSLINEQFEFVRALPPAIKERLLVRLHGPDENLDRF
                     GDELRWNDFDRTVNLERGKVLFADVVRKSRIVVFGYDSTGMLESLASNSPMIAFWSGG
                     LSHLKDHVKPQYEWLIDAGIVHLSGESAARKISEIWDNVNAWWQSESVQSARVRFCEI
                     YARTSRTPAADLLKIIKDDL"
     gene            1237..2439
                     /locus_tag="PKK76_07230"
     CDS             1237..2439
                     /locus_tag="PKK76_07230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNL68301.1"
                     /translation="MTTAFFLDNQYRGGVDTFLIQLINHWPEDGRLRLYCNRHHPGLE
                     TIRAMVHRPLQISTYGDSISWTLFSGKARIPLDRFYVVRAAVVVFQRIMPPILFLANL
                     IRFSVRFRMSGAHSLLVVNGGYPASATARSASLAWRIAGKGGVNIHNFHSHASPSNGK
                     AKRMNDFLDRLILRASTYFVTVSRSCELSLRKRDVFLNSDRIRVIPNGIADPGFPLHR
                     PETQKLSCLMLGTYHEYKGHSFLLEAFAQIRERHATTELNMYGDGSEADRVRIEGLID
                     RFGLRECAFLHGFVTDPDALIRNADVLVLPSQAHESFGLTIVEAMARGVPVVATRVGG
                     IPEVLEDGVQGFVCSPSDASAFAAAISRLLSSEELRRDLGAAGRRRYEQHFRAEQMSK
                     AYHSLLCS"
     gene            2497..4218
                     /locus_tag="PKK76_07235"
     CDS             2497..4218
                     /locus_tag="PKK76_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNL68302.1"
                     /translation="MYILGIHDGHNSAACLLKDGKLVFAIQEERLTGIKNYWGHPLKA
                     IDACLKHEGITWAQVDHVAFAAFDGSSPGRPFISRDEYLEYTKHVLKPAPRAGLRARL
                     RSVLRPAKAVAVPDRLSFYPPEIAAKGNAVEKIRHHLCHVATAAFGSNIGDGHDLLIV
                     TVDGLGDGECATVNVLRADGQIEPQLSVHQAHSFAQIYAFVTVYLGFVHLEHEYKLMG
                     MAPYGRDRDAERIADKLEGLFTYTDARWELKTPVWNLNSDERLVYESLREMFEFERFD
                     NICAGLQLFSERFIVRFIQDWIRKTGVRRVAVSGGFFMNVKANQKLRSLPEIEQLFVY
                     PSCGDETNAMGAAYLKYFQLTGKRPEPLKNFTLGPVIDDSTLLDVLKSYGEKIRFEYC
                     EDIEERAADLLSKKNIVARVKGPEEFGARALGNRSILAHPSDTLAVRTINDMIKSRDF
                     WMPFACSILEEEKLRYLKDWTANFSPNYMTMTYDSKNSEEILAGMHPRDRTVRPQVVT
                     REQNADYHRLLSRFHDMTGIGGVLNTSYNLHGFPLVHDASAAIEVFLNSGLEYLALNQ
                     YLVTKPG"
     gene            4222..4917
                     /locus_tag="PKK76_07240"
     CDS             4222..4917
                     /locus_tag="PKK76_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68303.1"
                     /translation="MISRLKQFIGGLLHRYRLGQEARRHRKEVEGKSVDVVFTEIFKR
                     NFWGDRESVSGSGSNQEQTRVIVREIPRVLKQVGARTLLDLPCGDFNWMQRVDLSGVS
                     YTGGEIVQDLVDLNQKKFGRDGISFKKMNLIDGDLPASDVLLCRDCLVHLSNEQIHAS
                     LANIKKHGIRYLLTTSFTETKKNSNIVTGEWRPINLEIPPFSLKAVEVINEECTEGNG
                     QFADKSLILVRMN"
     gene            4931..5365
                     /locus_tag="PKK76_07245"
     CDS             4931..5365
                     /locus_tag="PKK76_07245"
                     /inference="COORDINATES: protein motif:HMM:NF017345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HNL68304.1"
                     /translation="MIQSKKNPRIPTKNHDGKQNGFLIPIINVNEGFPSPDAWPKQVY
                     CTVAYPGQVKGPHLHKKRAGLFTCIKGNVKIVIRIDGKYVEHFSGDDHEYATIQVPAG
                     IPSALVNIGKEDAYILNMPSPAWSKDEPDDWDVSFEGYNFNA"
     gene            5362..6360
                     /gene="rfbB"
                     /locus_tag="PKK76_07250"
     CDS             5362..6360
                     /gene="rfbB"
                     /locus_tag="PKK76_07250"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNL68305.1"
                     /translation="MNRTLLITGGAGFIGSHLILRLFERYPDYRFVNIDKLTYASDES
                     YLDPIRSSGRYTFAKVDLVNKADVTAVFEEHKPDGIIHLAAETHVDNSISGPEVFIYS
                     NVVGTMNLLELARRTWAAGSANRFHHVSTDEVYGSAAPGSSFREDTPYSPNSPYSASK
                     AASDHLVRAYHTTYGLNVVVTNCSNNFGPHQHDEKLIPTIVRNALQGKDIPIYGKGEN
                     VRDWLFVKDHCAALDLVFHKGTAGETYNVGGENEWQNLALAKKILGLLDEIHPSSASY
                     LNQIRFVQDRPGHDLRYSVNCAKIQRELGWEGSAAHFDQALRETLSWYVRKYGGSR"
     gene            6357..>7086
                     /gene="rfbA"
                     /locus_tag="PKK76_07255"
     CDS             6357..>7086
                     /gene="rfbA"
                     /locus_tag="PKK76_07255"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNL68306.1"
                     /translation="MKGIIMAGGSGTRLLPLTTATSKQLLPVYDKPMIYYPISTLMLA
                     GIREILIITTGEHQSQFKELLGDGSSLGLRIQYAVQDQPRGISEAFILGADFIGKDSV
                     CLILGDNIFYGHGFVDMLKRSSQIEQGAVIYGYYVNDPQRYGVVEFDANLKVHGIEEK
                     PADPKSNYAVTGLYFYDNRAVEMARNLKPSLRGELEITDLNKLYLARGELRLELLGRG
                     FAWLDTGTHKSLMEASKFVQVIEER"
BASE COUNT         1716 a         1726 c         1842 g         1802 t
ORIGIN      
        1 gggcgaaagc tatcgccttc ctggacattt tgggcctccg cctcgcatgt ctctggcgcg
       61 cagactgcga agagtacctg gtttgatccg ccagcgcctg gttgtgcgcg ggacggattc
      121 ctttatcttc aacacataca tgcaggcgga ggaagaagtc cttcttcaaa gagggctcgg
      181 gcaaactcca cggcgctggc attcgcatca tttttttggc ccttctgtgg atcctgattt
      241 gcgcagggaa ttgtccgcga gtttctccgg agactcttcc ggtttgatgg gatgcatgcg
      301 agcgcttctt ttcgaactca ttcctgtttg ctacctggaa ggataccgcg acctggtgaa
      361 gaaatcggaa tccatgccct ggccagatcg gcccaggctt attttcacga gtggcaacta
      421 tgacacggac gaaatcttca aatgctggac ggcttcacgc gtagaacgtg gctcgcgcta
      481 catggttggt caacatggaa acaactatgg aacgcaccgt tatatggcat atcgcacgac
      541 agaccagcta acgtcggatc gatttctgac atggggctgg agggacagcc ttccttcgca
      601 tacacctgca ttcattttgc gcacttccgg ttttcctgag ctggatccag caggaggtct
      661 ccttctggcc gaggatggtc tacccaatcg cgtctatacc tgggacatct ttcctgacta
      721 cacgagcctg atcaacgaac agtttgaatt cgtgcgcgca ctgccccccg cgattaagga
      781 aagactgctg gtacgcctgc acggtccaga tgaaaacctc gatcgatttg gagatgaact
      841 caggtggaat gattttgata ggacagtgaa cctggagcga ggcaaagtcc tcttcgcaga
      901 cgttgtgaga aagagtcgca tcgtagtgtt tggttacgat tccacgggta tgctggagag
      961 tcttgcttcc aatagtccca tgattgcatt ctggtcaggc ggcctttcgc atttaaagga
     1021 ccacgtaaag cctcagtatg aatggttgat cgatgcggga attgtccatc tgtccggtga
     1081 gtctgctgcg cgaaagataa gcgaaatatg ggacaatgta aacgcctggt ggcaaagcga
     1141 atcggtgcag agcgcgagag tccgcttctg tgaaatctac gcgcgaacat cccggactcc
     1201 tgcggcggac cttctaaaaa tcatcaagga cgatctgtga ccacggcttt tttcctggac
     1261 aatcagtacc gtggaggagt agacacattc ctgattcaat tgatcaatca ctggcccgag
     1321 gacggtcgac ttcgcctcta ttgcaaccgg catcaccccg ggctagagac aattcgagcc
     1381 atggttcatc gtccgctgca aatatccacg tatggagatt cgatttcgtg gacactgttt
     1441 tcggggaagg ctagaattcc tctggatcgc ttttatgtag ttcgcgcggc cgttgtggtt
     1501 tttcagagaa tcatgccgcc gattctcttt cttgccaacc tgattcgttt ctccgtgcgt
     1561 tttcggatga gcggtgcgca ttcccttctt gtagtgaacg gcggctatcc cgcaagcgcg
     1621 acggcacgaa gcgcgtccct tgcctggagg atcgcaggaa agggcggcgt taacatacat
     1681 aattttcaca gccacgcttc gccttcgaac ggaaaggcga agagaatgaa cgattttctt
     1741 gacagattga ttttgcgtgc gtctacgtac tttgtaacgg tttctcgcag ttgtgaactt
     1801 tcgctgcgaa agcgagacgt ttttctaaac tcggatcgga ttcgcgtgat ccccaatgga
     1861 attgccgatc caggatttcc tcttcacaga ccagagactc agaaattgag ttgcttgatg
     1921 ctcggaacct atcacgagta caaaggccat tcgtttcttc tcgaggcttt cgcgcagatc
     1981 agggaaaggc acgccacaac ggaactcaat atgtatggag atggatcgga agcggatcgc
     2041 gtccgcattg aagggttgat tgatcgtttt ggactgcgcg aatgcgcgtt cctgcatggc
     2101 tttgtaactg accccgatgc tctcattcgc aacgcagatg tactggttct gccatcccag
     2161 gcgcacgaat catttggact gacgatcgtt gaagccatgg ctcgtggtgt tcctgttgtg
     2221 gctacgcgcg tcgggggcat tccagaggtg ctcgaagatg gcgttcaggg ttttgtttgt
     2281 tcgccgtctg atgcgtctgc atttgcggcc gcaatttcgc gtctgctttc ctcggaggaa
     2341 ttgcgtcgcg atctcggggc ggcgggtcgt cggcgctatg aacagcattt tcgcgctgaa
     2401 cagatgagca aagcatatca cagtctcttg tgttcttagc tctaaattca atttgactcg
     2461 gggccgcacc ggcttccact tgtagttctc ttcaatatgt atattcttgg cattcacgac
     2521 ggtcacaata gcgctgcctg tcttctcaaa gacggcaaac tggtctttgc tattcaggaa
     2581 gagcgcctaa cgggcattaa gaactactgg ggacatccgc tcaaggcaat cgacgcatgc
     2641 ctgaagcatg aaggtattac ctgggctcag gtggatcatg ttgcatttgc cgcttttgat
     2701 ggatcttcgc ctggtcgtcc atttatctcc agggatgaat atctcgagta cacaaaacat
     2761 gttttgaagc ccgctccccg cgccgggtta cgagcgaggc ttcgcagtgt cttgcgtccc
     2821 gcgaaagctg tggctgtgcc ggatcgtctc agcttctatc caccggaaat agcagcaaag
     2881 ggaaatgccg tagaaaaaat tcgccaccat ctgtgtcacg tggctacggc tgcgtttggg
     2941 tcaaatattg gggatgggca tgatctactc attgttacag tagatggtct cggagatggt
     3001 gaatgcgcca cggtgaacgt actgcgcgcg gacggacaga tcgagccaca gctcagcgtt
     3061 catcaggccc attccttcgc acaaatatac gcctttgtca ctgtctacct tggatttgtt
     3121 catctggagc atgagtacaa gctcatggga atggcgccct acgggcgcga tcgtgacgca
     3181 gaacggatcg cagataaact cgaagggctt tttacttaca cagatgcacg ctgggaattg
     3241 aaaactccag tgtggaatct caattcagat gagcgcctgg tttacgagtc tttgcgtgag
     3301 atgttcgaat ttgaacgctt tgacaatatc tgcgctggtc tgcagctttt ctctgagaga
     3361 ttcattgtcc ggttcattca ggattggatt cgcaaaacag gtgttaggcg agtcgctgtt
     3421 tctggcgggt tcttcatgaa cgttaaggca aatcagaaac tgcgttccct gcctgaaatt
     3481 gaacagctct ttgtctatcc ttcctgtggt gatgagacca atgcaatggg cgccgcttat
     3541 ttgaagtact ttcaactcac cgggaaaagg ccggagcctc tgaagaactt cacgctgggt
     3601 cctgtaatcg atgattcgac tctcctggat gtgctaaaat cgtatgggga aaaaattcgc
     3661 tttgagtact gcgaagatat cgaggagcga gcggcggacc tgctatcgaa aaagaacatt
     3721 gtggctcgtg tcaaaggccc tgaagaattt ggcgctcgcg ctcttggcaa tcgttctatt
     3781 ctggcgcatc cctctgacac tcttgcagtg cgaaccatca atgacatgat taagtccaga
     3841 gatttctgga tgcctttcgc ctgcagtatt cttgaagagg aaaagctgcg ttacttgaaa
     3901 gattggaccg ccaacttttc ccccaactac atgaccatga cgtatgactc caaaaattca
     3961 gaggagatcc tcgcaggtat gcatcctcga gatcgcactg tgcgtcctca agtggtgact
     4021 cgcgaacaga atgctgatta ccatagactt ctgtcccgtt ttcatgacat gacagggatc
     4081 ggtggcgttc tcaatacttc gtacaatctc cacgggtttc ctctcgtgca tgacgcgagc
     4141 gccgccattg aggtcttctt gaattctggc cttgagtacc tcgcgctgaa tcagtacctg
     4201 gtgaccaaac ctggctagat tatgataagt cgtttaaaac aattcattgg tggattgctt
     4261 caccggtata gacttggcca ggaagcacga cggcatcgaa aagaagtcga aggcaaatca
     4321 gtggacgttg tgttcactga gatattcaaa cgcaacttct ggggtgatcg ggagagcgtc
     4381 tctggaagcg gaagcaatca ggaacaaaca cgcgtcatcg ttcgcgaaat tccgagggtg
     4441 ctcaaacagg ttggcgctcg cacattgctg gatcttccct gtggggattt taactggatg
     4501 cagagagtag atctatccgg tgtttcctat actggcggag agattgttca ggatcttgtg
     4561 gatctcaatc agaagaagtt tggacgtgat gggatttcat ttaagaagat gaacctgatt
     4621 gacggggatc ttccggcgag tgacgttctt ctatgtcgcg actgccttgt gcatctttcc
     4681 aatgaacaaa ttcacgcgag tcttgcaaat atcaagaagc acggcatccg ttatctgctc
     4741 actacatcgt tcacggaaac caaaaagaat agcaacattg tcaccggaga atggagaccg
     4801 atcaatcttg agatcccacc tttttccctc aaagctgtgg aagtcatcaa cgaagagtgc
     4861 acagaaggaa acggccaatt cgcagacaaa agtctgatcc tagttcgcat gaactagccc
     4921 ggaggacggt atgattcaat ccaaaaaaaa tccacgcatt cctacaaaga atcacgacgg
     4981 aaaacagaac ggttttttga ttccaatcat caatgtgaac gaaggttttc cttcgccgga
     5041 tgcatggcct aaacaagtgt actgcacagt cgcctatccc ggacaggtca aaggcccaca
     5101 tttacacaag aagcgcgccg gtctttttac gtgcatcaag ggaaatgtta agatcgtcat
     5161 tcgaatcgat gggaaatatg tagaacactt cagcggcgac gatcatgaat atgcaaccat
     5221 ccaggtcccg gcgggaattc catccgcgct cgtgaatatt ggcaaagaag acgcttacat
     5281 tttgaatatg ccttctcccg cctggagcaa agacgagcca gacgactggg atgtttcgtt
     5341 tgaaggttac aattttaacg catgaatcgc acgcttctga ttacgggtgg agccggtttc
     5401 atcggttcgc atttgatcct gaggctcttc gagcgctacc ctgattatcg cttcgttaat
     5461 attgacaagc tgacgtacgc ctcagacgaa tcttatctcg atccaattcg ttcgtccggt
     5521 cgctacacat tcgcgaaagt cgatctggtg aataaggcgg atgtaactgc ggttttcgaa
     5581 gaacataagc cagacgggat cattcatctg gcagcagaaa cacatgtaga taattccatt
     5641 tccggaccgg aagttttcat ttactccaat gtagttggca caatgaatct actggaactg
     5701 gcaaggcgca cctgggctgc aggaagcgcg aatcgatttc accatgtgag cacggatgaa
     5761 gtatatggct ctgcggcacc gggcagttca tttcgcgaag acacgcctta ttccccgaat
     5821 tcgccctaca gtgcatccaa ggctgccagt gatcacctgg ttcgcgcata ccacactacg
     5881 tacgggctca atgttgtagt aaccaactgt tccaataatt ttggacctca tcagcatgat
     5941 gaaaaactga ttccgaccat tgttcgaaat gcgctccagg gaaaagacat tccgatttat
     6001 ggcaagggcg agaatgtgcg cgactggctt tttgtgaaag accattgtgc cgcgctcgat
     6061 cttgttttcc acaagggcac tgcgggtgag acatacaatg tgggcggtga aaacgaatgg
     6121 cagaatctgg ctcttgcaaa aaagattctt ggccttctgg acgaaatcca tccaagctcc
     6181 gcatcctatc taaatcagat tcgatttgtg caagatcgac ccggtcatga tttgcgttac
     6241 tcagtcaact gtgcgaagat acagcgcgaa ctcggatggg aaggatcagc tgcacacttt
     6301 gatcaggcct tgcgcgaaac gttaagctgg tatgtgcgca agtacggagg ctccaggtga
     6361 aaggaatcat catggcaggt ggcagcggca cacggctgct cccactaaca acggctacat
     6421 ccaagcagct tttgcctgtt tacgacaagc cgatgatcta ctatcccatt tctactctga
     6481 tgcttgcagg gattcgcgag atcctgatca tcactaccgg tgaacaccaa agtcaattca
     6541 aggaactcct gggcgatgga tcgagtctcg ggcttcgcat tcagtatgca gtgcaggacc
     6601 aacctcgcgg cattagcgaa gcattcattc tgggcgcgga ttttattgga aaagatagcg
     6661 tctgtctgat tctgggcgac aatatctttt atggacacgg cttcgtggat atgttgaagc
     6721 gatccagtca gatcgagcag ggtgctgtga tctatggata ctatgtgaat gatcctcagc
     6781 gatacggagt cgtagaattc gatgcaaacc tgaaggttca tggaatcgaa gagaagcctg
     6841 cggatccaaa atcaaactac gcggttacag gactctattt ttacgacaat cgcgccgtgg
     6901 agatggcgcg aaacctgaag ccttctttgc gtggcgaact tgaaatcacg gatttaaata
     6961 aactgtacct cgctcgcggt gaactgcgac tggaacttct gggccgcggt ttcgcatggc
     7021 tagacacagg tactcacaaa agcctgatgg aagcctccaa gtttgttcaa gtgattgaag
     7081 aaaggc
//
LOCUS       DAORFF010000138         7084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5378_length_7084_cov_4.042538, whole genome shotgun sequence.
ACCESSION   DAORFF010000138 DAORFF010000000
VERSION     DAORFF010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5378_length_7084_cov_4.042538"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PKK76_07260"
     CDS             complement(<1..542)
                     /locus_tag="PKK76_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HNL68307.1"
                     /translation="MKRLFLMAAMFAIATSLQAQALSGTCVVLVHGILGFDDTNGLAG
                     GLVKYWGGVDTYLRNQGAKVATPGSSATNDVPTRSAQIKTYLNTWMPANGCTKVHLVG
                     HSQGGLVSRYLVSNLGYGSKVLSVTTVDSLHKGAPMADIVLGVIPSWLQPFANSALSL
                     LASLVYRDGRPQDAIAMGKSL"
     gene            complement(704..1021)
                     /locus_tag="PKK76_07265"
     CDS             complement(704..1021)
                     /locus_tag="PKK76_07265"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HNL68308.1"
                     /translation="MMDRSHELFVRFVDQMASGRFYEAHESMERLWALCGYEPASPYR
                     GLTQLAVALAHLEKENFEGAQSVLAKARKILFENEADFKSAVEEVQRRIDRRVSSDAG
                     INL"
     gene            complement(1018..1992)
                     /locus_tag="PKK76_07270"
     CDS             complement(1018..1992)
                     /locus_tag="PKK76_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68309.1"
                     /translation="MTLEEKRKVKQAAATFVVLFVLFNILISARILTHRLPQNQWQLN
                     TIRAETYVFKKNTWKYVVLGSSLTFRIAPGVDREDAYVMAFAGDSAFTGLEIIRRSGK
                     VPEYIFVEVNTLGMQPDEGKIQPLFSLQGKLRRAVHAFREENTPLNFIMYPVARAILG
                     ISEGISNLAGRIAPSYKEEEKKWSTYDPFRGAVPPENKKAGEPIPQKVESEPVIVPEN
                     LRSTAETKKVEEALIRLQGAVRDVESRGAKVVFFEMPMLPSRRTDSYKMLKLKLDELF
                     PPDHYKRVPQADDRNLVYSDGLHLDMPSSLSYAKFLMGFFPDAKPPSR"
     gene            complement(2004..3419)
                     /locus_tag="PKK76_07275"
     CDS             complement(2004..3419)
                     /locus_tag="PKK76_07275"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNL68310.1"
                     /translation="MLFNSYSFLLFLGVTFLLYYTPFLRKYQRQLLVFASFFFYSFEK
                     PVLLFLLLASILINSICSYWIIHARDSYRKATLAIGLIANLSLLAFFKYAPLISSTFS
                     AFGSSNDFHDFIAGIPLPLGISFYTFQGISLLVDCYRMHEAGTDEVIRIDSFPSHLLH
                     TTLFISFFPHQVAGPIVKAHLFFPQIKNKSLKDIEIYAAFRTIVTGYFLKTVIADNLK
                     DQTFWLDYPYFQYFSTTILFTLLFGFSIQIFSDFAGYTLIAIGIARLFGYELTRNFLF
                     PYIAISFSDFWKRWHISLSSWLRDYLYIPLGGNRANLLRTCINMMIVMILGGLWHGAA
                     WGYAIWGAFHGILLVAERLLARFIKLPESLFVTGLRILFVFVMVSFAWTLFRIHEWQH
                     VVLFISSVKNNIHIPTDYAAIAAVLLLSLPVFVYHIMYLVRENPSGILIKKYEGILYG
                     CMIFFIIFNAGNPGKFIYFQF"
     gene            3677..4861
                     /locus_tag="PKK76_07280"
     CDS             3677..4861
                     /locus_tag="PKK76_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68311.1"
                     /translation="MKHSIPGLILIVCLSTLLARPTNVPFFAGNGQMFVKVGAMGEVS
                     VWGHHSSLGPGDGTRATNADSPVRLGVPRALSAAAGNSHVLILLADGTIIGWGTNAEC
                     EVGNGNPGKPLKRWERLEEVRKPVPVTGITGVRMIAASDKISGAVREDGTVWIWGSGN
                     NGLGGNGIMEPQGSTVACIPVPRKVEGLGQIRSLSLSQTHAIAVGTDGSVYTWGMNGY
                     GQLGDGTSTHRARPARVEGIEDALDAFAGGTMSAVVLKDGSVLTFGINSNGQLGDPLT
                     NPSDDSKRYKRKPYKVPGVTGVVAISGGPGHIIAQLKDGRLIGWGEGYHGSLGNGSSD
                     GIFPVPVSPTGLGPVVAHYKALRTSFAVLADGRVFVWGPLRMNTGAGVLAGSATPVAF
                     RP"
     gene            5007..>7084
                     /locus_tag="PKK76_07285"
     CDS             5007..>7084
                     /locus_tag="PKK76_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNL68312.1"
                     /translation="MAFLFCATLAGSEARPLSVSIGRFEAFHSATRPDLEIRLRASLQ
                     KSLERNGIKGILLSASGDALAEARSQKTDFLVSGQYSSAKGLSLSAQIYDAQEGVVID
                     AFSLSDERASLSGLLSESEIAALGPPNEPETVENFSARIASRILTNPNKTRRPENISQ
                     FLDGTAVGRELKFPRSVVSVRDDAFRMLEEQRIAIGTRGTQTLREVPATIRVITDEDI
                     RRHRYQYLEDLLRDQPGFDMIFFQGVYGPIFMQQGIDGPENFRTLVFVDGVPDNNISS
                     GSAYIKHKYRLHNVKRVEILYGPASSLYGANAFSGVINIITKDGSDIQKVIEAGVGSL
                     YYESTFHRPAPNGFLTIGGVAMPGEDAISVIASAHWINSEGPILNQKSNVDPTKSTYY
                     WTDGYSNSRLSDNYMASLKMQYRSVTLGMWQERDYTGQGTFASAAAYLDDGRKTFWNT
                     LTTTYYGKFDQPIGMLQEHLQASYRETSVTDGADVDRSTIGSFNGKVTATRYRRPDSE
                     ISLDNPLQIKWSNNSYTTVGAAYVRQQPWTYNTRAVTYDSTAALYLIGMPASPDLDPR
                     NKFYATSKAGYAEHYMRLAGAFGTSVGYRYDHFTIEGKDGPEFCGTDEISLPGGAPNP
                     HFISPEAAQAAGCVLNERRQYYSHRAGDRNYDSSNPRIGLIYSSSSLLTAKLSVGEAF
                     RVPTVRELYSL"
BASE COUNT         1899 a         1778 c         1789 g         1618 t
ORIGIN      
        1 agcgatttgc ccattgcaat tgcatcctgt ggacgaccat cgcgatacac aagcgatgca
       61 agcaggctaa gcgcgctgtt tgcgaaaggc tggagccagg atggaatgac accaagaacg
      121 atgtctgcca ttggcgcacc tttgtgcagt gaatcaacgg tagtaacaga aagaactttg
      181 gagccgtatc caaggtttga aaccaggtaa cgagatacaa gtccaccctg agaatgtcca
      241 accaggtgaa ccttggtgca accgttagcc ggcatccatg tgttcaggta ggttttgatt
      301 tgcgcagaac gagtaggaac atcgttcgtt gcagagcttc caggtgtagc cacctttgcg
      361 ccctgattcc taaggtaagt atctacgccc ccccagtact taacgagacc accggccagg
      421 ccgtttgtat cgtcaaaacc caggatgcca tgaacgagaa ccacgcaggt tccggacagt
      481 gcttgagctt gtagtgatgt agcaatggcg aacatagccg ccatgagaaa cagacgtttc
      541 atttgatttc cccctgtaca cattaggcca aacgcatcag gtttttgtca agcaaatgac
      601 gattcctgac ataatgcaag gccctccccc cagggctggg ggaccctggg atcgccgcca
      661 ttttttgata gtcgattttc gaaataatcg gacgtttttc gggctagaga ttgattccgg
      721 catcggatga gacgcgacga tcaattcgcc tctgcacttc ttccacagca gatttgaagt
      781 ctgcttcatt ttcaaataga atctttctag ctttcgcaag aacgctctgc gctccttcaa
      841 agttttcctt ctccagatga gccagtgcga cagcaagttg agtcagcccg cgatatggac
      901 ttgcaggctc ataaccacac agcgcccaga gtctttccat ggattcgtgc gcttcataga
      961 atcgcccgga ggccatctga tctacgaacc gtacaaatag ctcatgcgaa cgatccatca
     1021 tctggatgga ggcttagcgt ccggaaaaaa ccccatcaga aatttagcat agctcaaaga
     1081 actcggcata tccagatgca atccatctga atatactaga tttctgtcat ctgcctgcgg
     1141 aacccgtttg tagtgatccg ggggaaacaa ttcgtccagt tttagcttga gcattttgta
     1201 cgaatcggtt ctgcgcgaag gaagcatggg catttcaaag aaaaccactt tcgctccgcg
     1261 tgattcaacg tctctcactg ctccctgaag gcgaatgaga gcttcttcta ccttcttcgt
     1321 ctccgccgtg ctgcgcaaat tctcaggcac aatgacaggc tcagattcta ccttctgcgg
     1381 aattggttca cctgctttct tgttctccgg cggaacggca ccgcgaaaag gatcgtaggt
     1441 agaccatttc ttctcttcct ctttgtagga aggggcaatt cgtcccgcaa ggtttgaaat
     1501 gccctcactg attcccagaa tcgctcgcgc caccgggtac atgataaaat tgagaggagt
     1561 gttctcttct ctaaaagcgt gcacggctct gcgaagcttt ccctgaagag aaaaaagagg
     1621 ttgtattttg ccttcatctg gttgcatgcc gagcgtattg acttcaacga atatatactc
     1681 tggtaccttt ccgcttctgc gaatgatttc aagccccgtg aacgcggaat ccccggcgaa
     1741 cgccatgacg tacgcgtcct cacgatccac cccgggcgca atgcgaaacg tcaaagatga
     1801 cccgagcact acatacttcc aggtattctt cttgaaaaca taagtttccg cgcgaatggt
     1861 attgagctgc cattggttct gcggcaatct atgagtgagg atgcgcgcgg agatcaggat
     1921 gttaaaaagc acgaacagca ccacgaacgt tgccgcagcc tgttttactt tacgtttttc
     1981 ttctaaagtc atccttaaac ctcctaaaac tgaaagtaga tgaactttcc gggattcccg
     2041 gcgttaaaga taataaagaa gatcatgcaa ccgtaaagga tcccttcata tttcttgatc
     2101 aaaatgcccg atggattctc ccgaaccagg tacatgatgt gatacacaaa aacaggaagc
     2161 gaaagcaaaa gcaccgcagc gatcgctgcg taatcagttg ggatgtgtat gttgtttttt
     2221 accgaggaga tgaatagaac tacgtgttgc cattcgtgaa ttctaaacaa cgtccacgcg
     2281 aacgatacca tcacaaagac gaacaaaatt cgtagccccg tcacaaacaa tgattcagga
     2341 agtttaataa accgcgcaag gagcctctcc gctaccagca ggattccatg gaatgctccc
     2401 cagattgcat agccccatgc agccccgtgc cacagtccac cgaggatcat cacaatcatc
     2461 atattgatgc atgtccgcag taggttcgct cgatttccgc cgagtggaat gtagagatag
     2521 tcacgaagcc agcttgaaag cgaaatatgc cagcgcttcc agaaatcgga gaatgaaata
     2581 gcgatgtaag gaaacaggaa gttgcgcgta agttcatatc cgaagagacg cgcgatacca
     2641 atggcgatca aagtatagcc ggcgaaatcg gaaaaaatct gtatggaaaa accaaacaac
     2701 agagtaaaca gaatggtggt tgaaaaatac tgaaaatacg gatagtcgag ccagaatgtt
     2761 tgatccttca gattatctgc tatgactgtc ttgagaaagt acccggtcac tatggtacgg
     2821 aacgccgcat agatttcaat gtccttcagt gatttgtttt tgatttgcgg gaaaaacaaa
     2881 tgcgccttca caatcggtcc agccacttga tgcggaaaaa aagaaatgaa cagcgtggtg
     2941 tgcaagagat gcgaaggaaa actatcgatt cgaatcacct catctgtacc cgcttcatgc
     3001 atgcgataac aatctaccag caggctaatt ccctggaaag tgtagaatga aattcccagg
     3061 ggcaagggaa tgcctgcgat gaagtcgtga aaatcattgg aactgccaaa cgcggaaaaa
     3121 gtggagctga tcaaaggtgc gtacttgaag aaagcaagca gcgacaggtt tgcaatcaat
     3181 ccaatggcaa gcgttgcctt gcgataagag tctcgcgcat gaatgatcca gtagctacat
     3241 atagaattga tcaaaatgga cgcgaggagt aagaacagaa gcactggctt ttcaaatgag
     3301 taaaaaaaga aactggcgaa aacaagaagc tgtctctggt atttgcggag gaagggcgtg
     3361 tagtagagaa gaaacgttac accaagaaaa aggagaaagc tgtagctatt gaagagcatt
     3421 gattggagag gctagcctca ctcgcaaagc atgccagtca agcaaaagga attggctgca
     3481 ttcgagaatc actcgcagga cacgaaatat ttgctggatg cttgttcttt tccatagtca
     3541 aaaaaatgcc tgccttgtct taacgagaga aggggcgaat tgccggctgc ccgggcttgc
     3601 cgtaaagcag gccgtgcgag gcgcaaatct ggcccggatc cgtactcgcg aactatctac
     3661 aatactgagg aaaaagatga agcattctat cccggggctg atcctaatcg tctgtttatc
     3721 cactctgctg gcaaggccga ctaacgttcc tttttttgcg ggcaacgggc agatgtttgt
     3781 aaaagtggga gccatgggtg aggtaagcgt ctggggacat cattcgtctc tgggaccggg
     3841 agacggcacc cgagcaacaa atgcggattc tcctgttcga cttggtgtgc cgcgcgcctt
     3901 atctgctgca gccggaaatt cacatgtttt gattttgctc gcagatggaa ccatcatcgg
     3961 ctggggaacg aacgcagaat gcgaagtggg aaacggaaat ccgggcaaac cactgaagag
     4021 atgggagcgc ctggaagaag tgcgaaagcc tgtgccagtg acaggaatca ccggagtcag
     4081 gatgattgcc gcttccgaca aaatatctgg agcagtacgc gaagacggca ccgtttggat
     4141 ctggggctca ggtaacaatg gtctgggtgg caacggcatc atggaaccac aggggtccac
     4201 tgtggcctgc attcctgttc cgcgaaaagt ggaaggcctg ggtcagattc gctctctttc
     4261 tctttcgcag actcacgcga ttgcagtggg tacagacgga agcgtttaca catggggaat
     4321 gaacggttac ggacagttag gcgacggaac atccacacat cgtgcgaggc cggctcgcgt
     4381 ggaaggcatt gaagacgcgt tagacgcctt tgcgggcggt acaatgtccg cagtcgtgtt
     4441 gaaagacgga agcgttctta catttggaat caactcaaac ggccagctgg gcgacccact
     4501 taccaaccca tccgatgatt ctaagagata caaaaggaaa ccttacaaag tgcccggagt
     4561 cacaggggtc gtcgccatat ccgggggacc gggccacatc attgcacagc tcaaagatgg
     4621 acgcctgatt ggatggggag aaggctatca cggctcgctt ggcaacggta gttcagacgg
     4681 cattttcccg gtgcccgttt ctcccaccgg actcggacct gtggtggccc attacaaggc
     4741 tcttcgcact tcgtttgcag ttctcgcaga cgggagggtt tttgtctggg ggcctcttcg
     4801 catgaacact ggcgccggtg tcctggctgg ttccgcgacg cccgtcgcgt ttcgaccgta
     4861 aagcatgcaa gagagccggt cgctgccggg accaaacacc tgatcattac gaatagttaa
     4921 ggatctataa tacctggcca ggccaggtta acggttgcaa agtccgggag ccatgccaaa
     4981 tttcggacgt ggcgcgtcgc ggttttatgg ccttcctttt ctgcgctacg ctggccggta
     5041 gcgaagccag gcctctcagc gtgtccattg gccgttttga agctttccac tccgcgacca
     5101 gacctgacct tgaaattcgc cttcgcgctt cgctgcaaaa aagtctggag agaaacggaa
     5161 tcaaaggaat tcttctgtcg gcttctggcg acgcactggc agaagcacgc tcgcagaaaa
     5221 cagatttcct ggtctctggc cagtactctt ccgcaaaagg actctcgctt tccgcgcaga
     5281 tctacgatgc gcaggaaggt gtcgtcattg atgccttcag tctctcggat gagcgtgctt
     5341 cactttctgg tcttctatca gaaagtgaaa tcgcggcact tggcccgccc aatgaacccg
     5401 aaacggtaga gaacttttcc gcacgaatcg cttcgcgcat cctgaccaac ccgaacaaaa
     5461 ccagaaggcc agaaaacatt tcccagttcc tggatggaac ggcagtggga cgcgaactga
     5521 aatttccacg ttctgtagtc agtgtgagag atgatgcgtt tcgcatgctg gaagaacaaa
     5581 gaatcgctat cggaactcgc ggcactcaga ctctgcgcga agtcccggcc accattcgag
     5641 tcatcacgga cgaagacatt cgcagacata gataccagta tctcgaggac ttgctgcgcg
     5701 atcagccggg ctttgatatg atattctttc agggtgtgta tggccccatc ttcatgcaac
     5761 agggaatcga cgggcccgaa aactttcgca ctctcgtatt tgtagatgga gtaccggaca
     5821 acaacatctc gtccggaagc gcctacatca aacataaata tagactgcac aatgtaaaac
     5881 gcgtagaaat actctatggt ccggcctcga gcctgtatgg tgcgaatgca ttttcaggcg
     5941 tgatcaacat catcacaaag gacggttcgg acatccagaa ggtaatagaa gcaggagtag
     6001 gaagccttta ctatgaatca acctttcaca ggccagcgcc caacggattt ttaacgatag
     6061 gcggcgttgc catgccagga gaggacgcaa tttccgtgat tgcctctgcc cactggatca
     6121 acagcgaagg ccccattcta aatcaaaaat cgaatgtaga cccgacaaag agcacctact
     6181 actggacaga cggctatagc aattctcgcc tgagcgacaa ctacatggca agcctcaaga
     6241 tgcagtatcg ctctgtgact cttggaatgt ggcaagaacg agattacacg ggacagggca
     6301 cattcgcgag cgccgctgcc tatctggacg atgggcgaaa gacattctgg aatactctga
     6361 ccaccactta ctacggcaaa ttcgatcaac ccatcggtat gttgcaagaa cacctgcagg
     6421 cttcctatcg cgaaacatcg gttacagacg gagcagatgt ggatcgcagt accattggca
     6481 gcttcaatgg aaaagtcacg gcaaccagat acagaaggcc ggactctgag atctctctag
     6541 acaatccttt gcaaatcaaa tggtctaata attcctatac gaccgtgggc gcagcctacg
     6601 ttcgtcagca gccatggact tacaatactc gcgcagttac gtatgattcc acagccgctc
     6661 tttatttgat tggaatgcct gcatcgcctg acctcgaccc tagaaacaaa ttctatgcaa
     6721 ccagcaaagc aggatacgcg gaacactaca tgcgtctggc aggcgcattc ggaacctcag
     6781 ttggctatcg ctatgaccat ttcacgattg agggaaaaga cgggcccgaa ttctgcggaa
     6841 cagatgagat ctctcttcca ggaggagcgc cgaatcctca ctttatttct ccggaggcag
     6901 cccaggctgc aggctgtgtt ctcaatgaaa gacgacagta ctattcgcat cgcgcgggag
     6961 atagaaacta cgattcttcc aatcctcgca ttggtctgat ttactctagc tcttctctgc
     7021 tcacggcaaa gctgtcagtg ggagaagcat ttcgcgtccc cactgtgcga gaactgtatt
     7081 ctct
//
LOCUS       DAORFF010000139         7069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5394_length_7069_cov_3.594953, whole genome shotgun sequence.
ACCESSION   DAORFF010000139 DAORFF010000000
VERSION     DAORFF010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5394_length_7069_cov_3.594953"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /gene="rplB"
                     /locus_tag="PKK76_07290"
     CDS             <1..418
                     /gene="rplB"
                     /locus_tag="PKK76_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNL68313.1"
                     /translation="NIELRAGRGGQIARSAGAFATVSGRDQEYIIIKLPSGEVRKIHE
                     ACNATVGIVGNKDHNLVSLGKAGRARWLGRRPKVRGVVMNPVDHPHGGGEGKTSGGRH
                     PVSPWGQPTRGYKTRKKRKTSDRFIVQRRKNKRIGE"
     gene            418..708
                     /gene="rpsS"
                     /locus_tag="PKK76_07295"
     CDS             418..708
                     /gene="rpsS"
                     /locus_tag="PKK76_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNL68314.1"
                     /translation="MSRSLKKGPYIAEHLMAKITKLNSENQKKPFKSWSRRSTVFPEM
                     IGHTILLYNGKSFIPVYIHENMVGHRLGEFAPTRTFRGHTSLDKKIKAKPGA"
     gene            711..1046
                     /gene="rplV"
                     /locus_tag="PKK76_07300"
     CDS             711..1046
                     /gene="rplV"
                     /locus_tag="PKK76_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNL68315.1"
                     /translation="METKSTGKFLLSAPRKIRLVADEIRGYSYPEAMDILRFIPRKGS
                     RYIESVLKTARSNAVSLQKDLREEDLYIKKVYVDMGPTLKRFRPRARGRGMRRLKRMS
                     HITIVLSNE"
     gene            1054..1731
                     /gene="rpsC"
                     /locus_tag="PKK76_07305"
     CDS             1054..1731
                     /gene="rpsC"
                     /locus_tag="PKK76_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNL68316.1"
                     /translation="MGQKVNPVGLRVGINRTWDSIWFAKESYRQNLHEDLMIRKMVQK
                     KFKRAGVVKIVIERFPEKIGINIHTVRPGTIIGPKGASIEALKAEVRKKITKPLNISI
                     VEVKKPETIAQTVGESIAQQLEQRMPFRRVMKQALRGAMRGGVEGVKIMVSGRLNGAD
                     MARQEQYKDGRVPLHTLRARVDYAHAEARTTYGIIGVKVWTYHGDALPGLEKAEDDDY
                     AVRRKTG"
     gene            1743..2156
                     /gene="rplP"
                     /locus_tag="PKK76_07310"
     CDS             1743..2156
                     /gene="rplP"
                     /locus_tag="PKK76_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNL68317.1"
                     /translation="MLAPQRVKYRKRQRGRLRGLSQRGSNVSFGEYGLKALDSGRLTA
                     RQIEAARRAMTRKVKRGGKLWIRIFPDTPVSRKPAETRMGSGKGNPEFWVSVIYAGRV
                     LFEISGVEEALAREALDLAAHKLPIRTVFVKKELI"
     gene            2156..2443
                     /gene="rpmC"
                     /locus_tag="PKK76_07315"
     CDS             2156..2443
                     /gene="rpmC"
                     /locus_tag="PKK76_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNL68318.1"
                     /translation="MARALHELADAELHKLVDDSQVEIRELRFQFGVARSLQNPSQIR
                     NLRRTIAGALTVLNLRKLGKAEQKAPTGKPAKEKKKAETKPQAKEKKKAKA"
     gene            2453..2722
                     /gene="rpsQ"
                     /locus_tag="PKK76_07320"
     CDS             2453..2722
                     /gene="rpsQ"
                     /locus_tag="PKK76_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNL68319.1"
                     /translation="MENTTKRTAKVRLIQGRVVSDTMDKTRVILLESRKMHPLIKKPL
                     VKSHRIKIHDAKNESKLGDVVLAVETRPLSRDKRHRLFKILEKAE"
     gene            2719..3111
                     /gene="rplN"
                     /locus_tag="PKK76_07325"
     CDS             2719..3111
                     /gene="rplN"
                     /locus_tag="PKK76_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNL68320.1"
                     /translation="MIQQQTYLDVADNTGARKVMCIKVLGGSRKRYAGVGDIIVVAVK
                     DAIPAYGIKDATGKKVHNKVVQKAVVVRTRKEIRRPDGSIIRFDDNACAIIDDKMNPK
                     GTRIFGPVARELRDRKFLKIVSLAPEVL"
     gene            3112..3438
                     /gene="rplX"
                     /locus_tag="PKK76_07330"
     CDS             3112..3438
                     /gene="rplX"
                     /locus_tag="PKK76_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNL68321.1"
                     /translation="MAAEFSTKLRKDDEVIVIAGKQKGKRGKIMSLDKAKARIIVQGV
                     NRVKRFQRPSQENPKGGTVELEQSMPISNVLFYDSKSKKGVRLGYKIQPDGKKVRVTR
                     PDGREV"
     gene            3438..3986
                     /gene="rplE"
                     /locus_tag="PKK76_07335"
     CDS             3438..3986
                     /gene="rplE"
                     /locus_tag="PKK76_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002722943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNL68322.1"
                     /translation="MSLLKKKYAESVIPQLQKQFAYSSVMQVPRFEKVVLNYGSGEAA
                     ANPKQFESAIEEMAQVSGQKPVKTIARKSIATFKVREGMVLGCKVTMRGDRMFEFLER
                     FITIALPRVRDFKGLNPSGFDGRGNYNLSLKEQIIFPEIDVDKVQNYHGMNVTIVTTA
                     PTNEEAFALLEAIGMPFSKGKK"
     gene            3994..4179
                     /locus_tag="PKK76_07340"
     CDS             3994..4179
                     /locus_tag="PKK76_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNL68323.1"
                     /translation="MAKKSLREKHKRKQAFKVREYNRCPVCGRPRAYMRRFDMCRICF
                     RKLAGFGQIPGVIKSSW"
     gene            4199..4594
                     /gene="rpsH"
                     /locus_tag="PKK76_07345"
     CDS             4199..4594
                     /gene="rpsH"
                     /locus_tag="PKK76_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNL68324.1"
                     /translation="MSLSDPIADMLTRIRNASRAKHPKVTFGGSRLKIAILDILKREG
                     FIEDYAVRKDKQKSELDVMLKYSGRQPVIRQLQRVSSPGRRFYIKATEVRPVRNHLGL
                     SILSTPKGVMTGREARTQNVGGELICRVW"
     gene            4608..5159
                     /gene="rplF"
                     /locus_tag="PKK76_07350"
     CDS             4608..5159
                     /gene="rplF"
                     /locus_tag="PKK76_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNL68325.1"
                     /translation="MSRIGNAPIQLPQGVDVEASGDKLKVKGPLGTLESPLPAGITLS
                     KEGGVVNLKRANDEMPLKQKHGMARAILMNSVIGVSKGWEKKLELVGVGYRAQMKGAD
                     LSFSLGYSHEVKFQVPAGIKVQVTDQVRLDITGIDRQKVGQTASEIRSLRPPEPYKGK
                     GIKYAGENIRRKAGKTGKAGKGK"
     gene            5163..5525
                     /gene="rplR"
                     /locus_tag="PKK76_07355"
     CDS             5163..5525
                     /gene="rplR"
                     /locus_tag="PKK76_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNL68326.1"
                     /translation="MNRIAQKQERLGRRRDRVRFAIRSRKARPRLVFNRSNRFLAVQL
                     IDDAKGVTLAHASTMEEAFKSGGTKNKAAAKKLGELMADRILKLGIKQIVLDRRGILY
                     HGKIAEFADAARAKGLEF"
     gene            5567..6067
                     /gene="rpsE"
                     /locus_tag="PKK76_07360"
     CDS             5567..6067
                     /gene="rpsE"
                     /locus_tag="PKK76_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNL68327.1"
                     /translation="MEKEFSEKVVKINRVSKVVKGGRRFSFNALAVVGDNHGRVGIGL
                     GKANEVPDAIRKAIESARRQLRHVRLTRKNTIPHEVVGRFKATRVTLLPATPGTGIIA
                     GEAVRAVVEQAGIHDVLSKVIGSKNPVNVVRATLDALEQMETPIMSARKRGVSLQHIF
                     GGKGAA"
     gene            6067..6255
                     /gene="rpmD"
                     /locus_tag="PKK76_07365"
     CDS             6067..6255
                     /gene="rpmD"
                     /locus_tag="PKK76_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNL68328.1"
                     /translation="MGEQVTITLVKSPVGRVPKHRGTLRALGLWKIGEKRSHKMTPQL
                     AGMIRTVSYLITVESAKK"
     gene            6269..6766
                     /gene="rplO"
                     /locus_tag="PKK76_07370"
                     /pseudo
     CDS             6269..6766
                     /gene="rplO"
                     /locus_tag="PKK76_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            6856..>7069
                     /locus_tag="PKK76_07375"
     CDS             6856..>7069
                     /locus_tag="PKK76_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNL68329.1"
                     /translation="MNALINIFKIPELRRKILFTAGLVIVYRLGSFITIPGVNPIAVA
                     QSRPPGQSFLDVVNVFSGGALFKLSIF"
BASE COUNT         1946 a         1673 c         1943 g         1507 t
ORIGIN      
        1 caacatcgag cttcgcgcag gacgcggcgg tcagatcgcc agatccgcgg gtgcgtttgc
       61 aaccgtatcg ggtcgtgatc aggaatacat catcatcaag cttccttccg gggaggttcg
      121 caagatccac gaagcgtgca acgctaccgt tggaatcgtt ggaaacaaag accacaacct
      181 ggtgagtctc ggaaaagcag ggcgtgcgcg ctggcttgga cgcagaccaa aagtccgcgg
      241 tgtggttatg aaccctgtgg atcacccaca tggtggtggt gaaggtaaaa cgtccggtgg
      301 tcgtcatcct gtcagcccat ggggtcaacc aacgcgcggc tacaagacaa gaaagaaacg
      361 caagacatcc gatcgtttca ttgtgcagcg tcggaagaac aagaggattg gtgaataatg
      421 tctagatctc ttaaaaaagg gccatacatc gccgagcact tgatggcgaa gatcacgaag
      481 ctgaacagcg agaatcagaa gaagccgttc aaatcctggt ccagacgctc tacagtgttt
      541 ccagaaatga tcggtcacac gatccttttg tacaacggca agagcttcat tcctgtttac
      601 atccatgaga acatggtggg tcacagactg ggcgaattcg ctccgacccg tacgtttcgc
      661 ggacacacca gcctcgacaa gaaaatcaag gcgaaaccag gggcataaga atggaaacga
      721 aatccacagg taagttccta ctctccgcgc ctcgtaaaat ccgtctggtt gcagacgaga
      781 ttcgcggata cagctatccg gaagcaatgg acattttgcg cttcatcccg cgcaaaggtt
      841 ctcgctacat cgagagcgta ctgaaaacag cgcgttccaa cgcagtttct ctgcagaagg
      901 atctgcgcga agaagatctc tatattaaga aagtgtatgt ggacatgggt ccaactctca
      961 agcgcttcag acctcgtgca cgtggccgtg gaatgagaag actcaagcgc atgagtcata
     1021 taacgatcgt actgagtaac gagtgaggaa tcaatgggtc agaaagtaaa tccagtcggc
     1081 cttcgcgtag gaatcaatcg gacctgggat tcgatctggt tcgcaaagga atcatacagg
     1141 cagaatctgc atgaggatct gatgatccgc aaaatggtgc agaagaaatt caagcgcgca
     1201 ggcgttgtga agatcgtcat cgaacgtttc cctgagaaaa tcgggatcaa cattcacaca
     1261 gtgcgtccag ggacaatcat tgggccgaag ggagcttcta ttgaagcgct gaaggctgaa
     1321 gttcgtaaga aaatcacgaa gcctctcaat atcagcatcg tggaagtgaa aaagcctgaa
     1381 acgattgcac agaccgtagg cgagtctata gcacagcagc ttgagcagag aatgccattt
     1441 agaagagtca tgaaacaagc acttcgcggc gcaatgcgtg gtggagtgga aggcgtgaag
     1501 atcatggtat ccggtcgctt gaatggcgcg gacatggccc gccaggaaca gtacaaagat
     1561 ggacgcgttc ctcttcatac tcttcgtgcg cgtgtggact atgctcacgc agaagcacgc
     1621 accacttatg gaattatcgg tgtgaaggtt tggacctatc atggtgacgc acttccaggt
     1681 cttgaaaaag ccgaagacga tgattacgca gtaagaagaa aaacgggtta acggaataga
     1741 atatgctggc gccccaaaga gtaaaatatc gcaaaagaca gagaggccgc cttagaggtc
     1801 tttctcagcg cgggagcaat gtctccttcg gtgaatacgg actgaaggct ctcgattccg
     1861 gtcgcttgac tgcgcgtcag atcgaagcgg ctcgccgcgc gatgacacgt aaagtgaagc
     1921 gcggtggaaa gctgtggatt cgcatcttcc ctgacactcc tgtttctcgt aaacccgctg
     1981 aaactcgtat gggtagcggt aaagggaatc ctgaattctg ggtatctgtt atctatgcag
     2041 gccgcgttct ttttgaaatc agcggcgttg aagaagctct cgcaagagaa gcgctggatc
     2101 tggcagctca caaacttcca atccgcacag tatttgtgaa aaaggagctc atctaatggc
     2161 acgcgcatta catgaactgg ctgatgcaga attgcacaag ctggtagacg attcgcaggt
     2221 tgaaattcgc gaactgcgtt ttcaatttgg agtggcgcgg tcgctccaga atccatctca
     2281 gattcgtaac ctcagacgca cgatcgcagg agcgttgacc gttttgaatc tgcgcaagct
     2341 tggcaaggca gagcagaaag ctcctaccgg gaagccagca aaagaaaaga agaaagcaga
     2401 gaccaaacct caggcgaaag agaaaaagaa ggcgaaagcc taaggcatga tcatggaaaa
     2461 tactacaaaa agaaccgcga aggtgcgtct gattcaaggt cgagtcgttt ctgacacgat
     2521 ggacaagacg cgcgttattc ttctggaatc gcgcaagatg cacccgctga tcaagaagcc
     2581 tcttgttaaa tcgcacagaa tcaagattca cgatgcgaaa aatgaatcca aactcgggga
     2641 tgtggtgctc gcagtcgaaa ctcgtccact gtcccgcgac aagcgtcatc gcctctttaa
     2701 gatcctggag aaagcagaat gattcagcag cagacatatc tagacgttgc ggataacact
     2761 ggcgcccgca aagtaatgtg tatcaaagta ctcggtggat cgcgcaagcg ttacgccggt
     2821 gtaggtgata tcatcgttgt cgccgtgaaa gatgcaattc ctgcatacgg gatcaaagac
     2881 gcaactggca agaaagttca taacaaggtt gttcagaagg cagtcgtggt tcgcactcgc
     2941 aaagagatcc gtcgtccaga tggctcgatc atccgtttcg atgacaacgc atgcgcgatc
     3001 atcgacgata agatgaatcc caaaggtact cgtatctttg gaccggtagc ccgcgaactt
     3061 cgtgatcgca aatttcttaa gatcgtatcc ctcgctccgg aggtgctcta aatggccgct
     3121 gaattctcaa ccaagcttcg caaagacgac gaagtgatcg tcattgcggg caagcaaaaa
     3181 ggaaagcgcg gaaagatcat gtctctggac aaagctaagg cgcgcatcat cgtgcagggt
     3241 gtgaatcgtg tgaagcgttt ccagcgccca agccaggaaa atccgaaagg cggcacagtt
     3301 gagctcgagc aaagcatgcc gatttccaat gtactatttt atgactctaa gtcgaagaag
     3361 ggcgtgcgtc tgggttacaa gatccagccg gacggaaaaa aagtccgcgt tactcgccct
     3421 gacgggagag aagtgtaatg tcccttctta aaaagaaata cgcagagagc gtgattccgc
     3481 agctccagaa gcagttcgcg tattcgtccg tgatgcaagt tccgcgattt gaaaaggttg
     3541 ttctgaacta cggatccggc gaagcggctg ccaatccaaa gcaatttgaa agtgcgattg
     3601 aagaaatggc gcaggtaagc ggccagaagc cagtgaaaac aatcgctcgc aagtccattg
     3661 caacgttcaa agtgcgcgag ggaatggttc tcggctgcaa agtcacaatg cgtggcgatc
     3721 gcatgtttga attccttgaa aggttcatca cgattgcact gcctcgtgtt cgcgacttca
     3781 agggcctcaa cccatcaggt ttcgacggcc gcgggaacta caatctttct ctgaaagagc
     3841 agatcatttt cccagaaatc gatgtggata aagttcaaaa ttaccacggg atgaatgtta
     3901 cgatcgttac cacggcgcct acaaacgaag aagcattcgc attgctcgaa gcgattggca
     3961 tgccgtttag caaaggaaag aagtgagggt atcatggcaa agaagagcct gagggaaaaa
     4021 cacaagcgga aacaagcctt caaagtgagg gaatacaacc gctgtccggt gtgcgggcgt
     4081 ccgcgtgcct acatgaggcg ctttgatatg tgccgtattt gctttcgtaa gctggccggt
     4141 ttcggtcaaa ttccaggcgt catcaagtcg tcctggtaaa acatttggag taatatagat
     4201 gagtctttca gatccaatag cagatatgct cacgagaatt cgcaacgcgt cgcgggccaa
     4261 acatcctaag gttacgttcg gcggatcacg tcttaagatc gcgatcctcg acatcctcaa
     4321 acgcgaaggg ttcattgagg attatgcggt tcggaaggac aaacagaagt ccgagctgga
     4381 tgtaatgttg aagtactctg gccgtcagcc ggtgattcgc cagctgcaaa gagtatccag
     4441 cccaggccgt cgcttctata ttaaagcgac tgaggttcgt ccggttcgaa atcacctcgg
     4501 actttcgatc ctctctacgc caaaaggcgt gatgacaggc agagaagcgc gcacacagaa
     4561 tgtgggtggc gaattgatct gccgcgtgtg gtaatcagga gaccggtatg tctagaattg
     4621 gaaatgcacc cattcagctg cctcagggcg tagatgtaga agcatctggc gacaagctga
     4681 aagtaaaagg tccgctcggg actcttgaat cacctcttcc tgccggcatt acgctgagca
     4741 aagaaggcgg agtggtgaat ctcaagagag ccaacgatga gatgccattg aagcaaaagc
     4801 atggcatggc tcgcgctatc ctcatgaact ccgtgatcgg agtgagcaag ggctgggaaa
     4861 agaagctgga gctcgttggt gttggttatc gcgcgcagat gaaaggtgcg gatctgagtt
     4921 tctcacttgg gtattcgcat gaagtaaagt ttcaggttcc tgccggaatc aaagtgcaag
     4981 taaccgacca ggttcgtctt gatattactg gaattgaccg ccagaaagtg ggtcaaactg
     5041 catctgaaat tcgttcgctg cgtccaccag aaccttacaa agggaagggg atcaagtatg
     5101 cgggtgaaaa catccgtcgc aaagctggta agaccggtaa agcggggaaa ggtaaataag
     5161 ccatgaatcg catcgcacag aaacaggaaa gacttgggcg ccgtcgtgac cgcgtccgtt
     5221 tcgctattcg ttctcgcaaa gcgcgtccaa gactcgtttt caatcgttcc aatcgcttcc
     5281 tcgcggttca actgattgat gatgcgaaag gcgttactct cgctcatgca agcacaatgg
     5341 aagaagcgtt taagtctggc ggaacaaaga acaaggctgc ggcaaagaaa ctgggcgagc
     5401 ttatggcaga ccgaattttg aagctgggaa tcaagcagat cgtactggac cgtcgcggca
     5461 ttttgtatca cggcaagatt gctgagtttg cagatgcggc gcgcgcaaaa ggactggagt
     5521 tttaattatg agcgaaagag aaagacatca acaccagcat caggaaatgg agaaggaatt
     5581 ctccgagaaa gttgtaaaga tcaaccgcgt ttcaaaagtt gtgaagggcg gtcgtagatt
     5641 ctctttcaac gctcttgcag tcgtgggaga caaccacggc cgcgttggaa ttggtctggg
     5701 caaagcgaac gaagtaccgg acgccattcg taaggcgatc gagtctgcgc gcagacaatt
     5761 gcgtcatgtt cgtttgactc gcaagaacac gattcctcac gaagttgtgg gtcgtttcaa
     5821 agcaacccgc gtaacacttt tgcctgcaac gcctggaaca gggatcatcg ctggagaagc
     5881 ggtgcgcgct gtagtagagc aggctggaat tcatgacgta ctgagcaaag taatcggctc
     5941 gaagaatcct gtgaatgtgg tgcgtgctac tctggacgca ctcgaacaaa tggagactcc
     6001 aatcatgtct gcgcgcaagc gcggcgtgtc tctccagcac atcttcggcg gtaagggagc
     6061 ggcataatgg gcgagcaagt caccatcact cttgttaaga gcccggttgg tcgcgtgccc
     6121 aagcatcgtg gaacgcttcg cgcactggga ctctggaaga tcggcgaaaa gcgcagtcac
     6181 aaaatgactc cgcagctggc cggtatgatt cgcaccgtgt cttatttgat cacggttgaa
     6241 tcagcgaaaa agtaaggata tattaacaat ggcaaagaaa gaaatcgcag ggctttccaa
     6301 tctgcttcct gctcgtaaaa aagcgaaggc gaaaagagca aaggcagccg tgagcattgc
     6361 tccaccggaa ggatctcgta aagaaggcaa gcgcatcgga cgtggccgcg ccaccggtgc
     6421 cggcaagact tcaggtcgtg gccagaaagg ccagaaagcg cgtgccggct acaagaagaa
     6481 gcttggcttc gaaggtggac agaacccact tcgtttgcgt attccaaaac gcggatttac
     6541 caatattttc gcgactcgct accaggtggt aaacctcaag gacctgacag aggccggctt
     6601 cagcggtgaa gtgaatccag aagcactcaa aggcaaaggt ttgatttcac gccccggtat
     6661 gcctgtgaag atcctcggag tgggaaccgt gtcgggtaag ctgtctgtta cagcggatgc
     6721 attctctgcg actgcgaaag cagccattga aaagtccggt ggaacctgca caataaggcc
     6781 tgattacatt cctcgcgtag accgtaagtt cgagaaaaaa gcccagcgca aaacgcgcct
     6841 tggtaaggga cgtgcatgaa cgcactgatc aacatcttca agatccccga gctccgtcgt
     6901 aagattcttt ttactgcggg gcttgtgatc gtttatcgcc ttgggtcctt cattacgatt
     6961 ccaggcgtca atccgatcgc agtggctcag tcacgcccgc caggccagtc attcctggac
     7021 gttgtgaatg tgttctcagg cggggcgctc tttaagttgt cgatcttct
//
LOCUS       DAORFF010000140         7056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5412_length_7056_cov_3.573775, whole genome shotgun sequence.
ACCESSION   DAORFF010000140 DAORFF010000000
VERSION     DAORFF010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5412_length_7056_cov_3.573775"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PKK76_07380"
     CDS             <1..1128
                     /locus_tag="PKK76_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNL68330.1"
                     /translation="ESTHIISSKYHSAYEDFPFPEGTADYPSHKELRRYFESYATHFG
                     VHGKIRFRTEVLSAIPEGDQWRITSKGPRGKKTETFDALMVANGHHWDPSMPALPGKF
                     SGRFMHSHDFKHARPFAGKRVLVMGGGNSAADVAVECSRVAAHTTISMRHGHWFLPKF
                     VFGVPNDVLYHRSLWLPGFIRQPLLRWTILLLQGSNSRYGLPEPSYDILEGHPTLNSE
                     LYYFIRHGEISPKPGIKSVKGSRVTFADDSSADFDVIIAATGYNIVFPFLDKSIVDLS
                     GKTEVGLWRKMFHPHYHNLYFIGLFQPLGCIWPLADFQAKLACQEILGKYERPSNIGK
                     AIDKEIKKPHYKFVRSPRHSTEVDYPRFRRELLTEIRKCRP"
     gene            complement(1109..2755)
                     /locus_tag="PKK76_07385"
     CDS             complement(1109..2755)
                     /locus_tag="PKK76_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNL68331.1"
                     /translation="MTGLGSLIFATLLEKGEGILLHSLVQFQHERPFDFSTLLDIRIL
                     GLLLAGALVNGIIIRFFAGEVRGSGIDFIIDRFHNHEGRMRGRVATYKSIATIASLST
                     GASAGKEGPAAQIGAGIGSLVANRIQIGPRARRTMLLAGAAGGLGAVFHAPLAGALTA
                     VEMLYREDVESDSLVPCIISSVSAYLVYTMIRGTGPIYPSTDLSFRALDLPFFAILGL
                     LCFVCGFVFVRTYEFISHSFTRSRIPLLLQPILGAILVFAVAAGFPQVLGSGAETLRG
                     LLAQPSFPATFTLCAWFLMLGFAKMIASSLTVGSGNSGGLFGPSLLMGAYLGLAVGTA
                     AHLFLPGQTASLHSYALVGMGAFYSGIASAPIAGMLMVCEMTGSYRLLPGLILVTTLA
                     LVLSQKWSIYKSQLHNRFQSPAHDWDIKMDFLERARLGESKIEIRRHAMVKVDATLAT
                     LRKLAARVHASDFVVFDKEHYSGMISLKQYQGRQNSRIRPDLTVPAVSPRDTLARALR
                     IMSERELDKVAVVDEGRILGYVRQRDVVGFYFSSVRGGTS"
     gene            complement(2888..3697)
                     /locus_tag="PKK76_07390"
     CDS             complement(2888..3697)
                     /locus_tag="PKK76_07390"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit B-like
                     domain-containing protein"
                     /protein_id="HNL68332.1"
                     /translation="MNARPLASVLMCHAPVAIPEIAGKNRDAAQKTTSAMKAAASFIT
                     TRSPDVVVIASPHTPRLKEEFVILKGDRLTGDFADFEFSDISVSVPSDQDAVSRLIAE
                     ANKSGIPVSVRYAASLDQGALVPLYFMKKAGWTGKTIVIGFPGRMPLRQFESLGRVIR
                     TVAQTRQETWAFIASGDMSHRLAPGSPAGYDQKAHNFDDSIVLALREGRLADALDVDP
                     ELRKLASEDVVESLAIAYGTSGGMRGGRVLSYESPFGVGYLVAILCDPDSE"
     gene            3751..4449
                     /locus_tag="PKK76_07395"
     CDS             3751..4449
                     /locus_tag="PKK76_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68333.1"
                     /translation="MRILIFCLCVLPTAADLYSQSVRGTARLACSDSLAELRKRKVPL
                     LENANTVFAFGYRQVAGLNQDPVLIRFDSGKRTYCRTDYETTQAEGRIYGLLLAGEDL
                     YAVASLVGPPGKADQDFRRFARKGWMRDSGKGSPRAAIILKIKAQSGDVESAAYLTSV
                     KEDGNSNSVEVLEMDLNSKGLTVKANAWLSPRMTSRAPMQCKGKEPYLQTIIFTPDLS
                     KTVDSFAERCVCCE"
     gene            4508..5446
                     /locus_tag="PKK76_07400"
     CDS             4508..5446
                     /locus_tag="PKK76_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL68334.1"
                     /translation="MIRAKKYGTIAAIAIAALLILKFILLDRSPVPETSPEAVNLKLV
                     RALARASRGELPQSLHVLIIAEGAFPKSAVIAGEGLFKKKPMVFDSFQAVFADKKTVI
                     IDTAMDAAGVEKMSKGMPFFADRYAAMQTAMTKAERIILTHEHPDHMGGIGKTEAMDK
                     IKDRLLLTKEQIDDPYRDKSYFTEDNIRAISSVKPLEYDPYFSPAPGIVLIRAAGHSA
                     GSQMIYVALKNGEEYLFVGDIVWSMENLEKLKGKPYLMNAAGFLKEDRAKVAAQIRFL
                     HNLTADPENKVHVVVAHDLEQIKDYIARGIVAEGFQ"
     gene            5525..>7056
                     /locus_tag="PKK76_07405"
     CDS             5525..>7056
                     /locus_tag="PKK76_07405"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNL68335.1"
                     /translation="MRTFKFSIVIVSILVALTACGGKKSKKGFLLFPFGAGMAVLDAN
                     GNALPENSGTSQQEAPTAGPAQISGSISPMNGSSAQTGFDFSGIEVSLVDPLGNVIAT
                     ATPDSFGVYTFDVPDLSNNNYRVLIPDGGGLNQGYVDFNFVFNPSQSGATLVALQPII
                     TQVQQFAAGPAKISGKALTPGYSDGVTTVSAGPLPAGTTVQLLDADDAVIASTTTNAS
                     GDYSFNIANLHNGNYSVKVIGSAQSASGRPFTDGSVGFHFAFEGYNIGTATQVNLSPA
                     QCAWTPATQAPASISGQVDNAANVAAASGLTVKLISSGGSVVAQTTTDGSGAFTLSAS
                     SVAGGIYRIEISGSAYNTQNASLLFVPLATGASTSVNAGSISVIPKASNIAGTIRDAA
                     TSAGIAGAVISFRPAASQPVSKLTYLLTDPVLGAAAARWIAEKNGTGSYSTYLTKSYN
                     GPDAGGNLYMTAVAGKWAYYVSAAGYQSSTAGEITLNGADETANISLTSDPKRSRISG
                     QAV"
BASE COUNT         1774 a         1800 c         1799 g         1683 t
ORIGIN      
        1 gaaagcaccc acatcattag ttcaaagtac cattccgcgt acgaagattt tccgtttccg
       61 gaggggaccg cagactatcc ttcacacaaa gagctcagac gttactttga atcctatgcc
      121 actcactttg gtgttcatgg caagatacgc tttcgcacgg aagtactttc agcgatcccg
      181 gagggagatc aatggcgcat aacgtcaaaa ggtccgcgcg gaaagaaaac agaaacattc
      241 gatgccctca tggtggccaa cggccatcac tgggatccgt ccatgcctgc tctgcccgga
      301 aaattctccg gtcgttttat gcattctcac gatttcaaac acgcgcgtcc ttttgcgggc
      361 aagcgagtgc tcgtaatggg aggtgggaat tcagcagcgg acgtggctgt ggagtgctcg
      421 cgggtagccg cacatacaac tatatctatg cgtcatggtc actggttttt gcccaagttc
      481 gtcttcggag tgcccaatga cgtcctctac caccggagtc tgtggcttcc cggattcatt
      541 cgtcagcctt tactccgctg gaccatcttg cttctacagg gctccaattc gcgatacggc
      601 cttcctgaac cgtcatacga cattctagaa ggacatccta ctctcaattc cgaactctac
      661 tacttcattc gtcacggtga aatatctcca aagccaggaa tcaagagcgt caaaggttcg
      721 cgtgttacat tcgcagatga ttcaagtgct gattttgatg taatcattgc ggccaccggc
      781 tacaacattg tgtttccatt tcttgacaaa tcgatcgtgg acctttccgg aaaaacggaa
      841 gtggggctat ggcgcaaaat gtttcatcca cactatcata acctgtattt cattggattg
      901 ttccagccat tggggtgtat ctggcctctc gcagattttc aggcaaagct cgcgtgtcag
      961 gaaatccttg gtaaatacga aagaccatct aacattggta aggcgattga taaagaaatc
     1021 aagaagcctc actataaatt tgtccggtcc ccgcgtcatt caacggaggt ggactatccc
     1081 agatttcgca gagaactcct gactgaaatc aggaagtgcc gcccctgaca gagctgaagt
     1141 agaacccgac tacatctctc tgtcgcacat agccgagaat tcttccttca tctaccaccg
     1201 ctactttgtc gagctcgcgt tcagacatga tgcggagtgc tcttgccaga gtatcgcgtg
     1261 gagaaacagc gggcactgta agatctggtc ggattcttga gttctgtctg ccctgatatt
     1321 gtttaagcga aatcattcct gaatagtgct ctttgtcgaa aacaacgaaa tcggacgcgt
     1381 gcactcgagc ggcaagcttg cgaagagtgg ccagagtcgc gtcaactttt accatcgcgt
     1441 gccgccgaat ttcaatcttg ctttcgccaa gtcgcgcccg ttccaggaaa tccatcttga
     1501 tatcccagtc atgagcaggg gattgaaagc gattgtggag ctggcttttg taaatgctcc
     1561 acttctgcga aagaacgagt gcgagcgtgg tgacaagaat caatccaggg aggagtcggt
     1621 agctgcctgt catttcgcat accattaaca tccccgcaat gggtgcactt gcaattccag
     1681 agtagaatgc gcccatcccc accagagcat acgaatggag gcttgcggtt tgcccaggaa
     1741 gaaacaaatg cgcggcagtt ccaaccgcaa ggccaagata ggcgcccatg agaagagaag
     1801 gcccaaataa tccacccgag ttgcccgatc caacagtgag actgcttgcg atcatcttcg
     1861 caaatccaag cattagaaac cacgcgcaca aagtgaacgt tgcgggaaat gacggctggg
     1921 ccaggagtcc gcgcaacgtt tctgctccgc tgccaagcac ttgaggaaag cccgcagcaa
     1981 ccgcgaaaac aagaatggcg cccaggatcg gttggaggag cagcggaatg cgagacctcg
     2041 taaacgaatg cgaaatgaat tcatacgtgc gaacaaaaac aaatccacaa acaaaacaca
     2101 gcagtcccag tatcgcaaag aagggcaaat cgagggcgcg aaagctcaga tctgtcgaag
     2161 ggtatatggg gccagtgcct cgaatcatcg tgtagaccag ataggcgctt acggaagaaa
     2221 taatgcacgg aaccagtgaa tcggattcga cgtcttcgcg gtagagcatt tcaactgcag
     2281 tgagtgctcc ggcgagcgga gcgtgaaaca cagctcccag acctcccgca gctccagcta
     2341 acaacatcgt tcggcgagcc cgcggcccta tttgaattcg atttgcaacg agcgatccaa
     2401 tgcctgcgcc aatttgagcc gccggtcctt ctttacctgc gctagcaccc gtgctcagtg
     2461 atgcaatcgt cgcaatggac ttgtaggtgg ccactcgccc gcgcattctt ccttcatgat
     2521 tgtgaaaacg atcaatgatg aagtcaatcc cgctcccgcg cacttctcct gcgaagaagc
     2581 gaatgatgat tccgttgacg agcgcacctg ccagaaggag ccccagaatt cgaatgtcca
     2641 ggagcgtact gaaatcgaat ggacgctcgt gctggaattg aaccagagaa tgaaggagga
     2701 ttccttcccc cttttccaga agagtggcga aaatcagaga tcccagtccc gtcaagactc
     2761 caatcaaaag gcagatgagg tagagagcgc ctgaagttct gataaaggac gggatgaggc
     2821 gtgcaaccac agcctacgtc agcgagcggc tgaaaaaacg caaatgaaat gtctttgcgc
     2881 aaaatcgtca ttccgaatca gggtcacaca aaatagcaac gaggtagcct acgccaaaag
     2941 gactttcgta ggacaacact cttcctcctc gcattccgcc tgatgttccg taagcaatcg
     3001 ccaatgactc gaccacatct tctgatgcaa gtttgcgcaa ctccggatct acgtccagtg
     3061 cgtctgccag ccttccttct cttaacgcta gaacaatgga atcatcgaag ttgtgtgcct
     3121 tctggtcata gcctgcgggg gatcctggcg caagtctgtg gctcatatca ccggatgcaa
     3181 tgaaggccca cgtttcttgc cgcgtctgtg caacggtgcg aatcactcga cccagggatt
     3241 caaattggcg cagaggcatc cttcccggaa aaccaatgac aatcgtcttt cccgtccagc
     3301 ctgccttctt catgaaataa agaggaacca gcgctccctg atcgagagat gccgcataac
     3361 gtacgctcac cgggattcca ctcttgtttg cttccgcgat cagccgggaa actgcatcct
     3421 gatcggaagg cacagaaacc gagatgtctg aaaattcgaa atctgcaaag tcgccggtaa
     3481 gtctgtcccc tttcaggatt acaaattcct ctttcagtct gggtgtgtga ggggaagcga
     3541 tcacaactac gtcaggcgat cttgttgtga tgaaggacgc tgccgctttc atggcggatg
     3601 tagtcttctg cgctgcgtcg cgatttttcc cggcgatttc agggatggcg acgggcgcgt
     3661 ggcacataag aacagatgct agtggccttg cattcatgga gggcttgacc gttcgtgctc
     3721 tcagtttaga tcggatgacg aatggatact atgcgaattc tcatattctg tttatgcgtt
     3781 ttacccacgg ctgcagatct gtattcgcag tctgtgcgcg gaacggcccg cctggcttgc
     3841 agcgattctc tggcagagct cagaaaacgt aaggtgcctc tgctcgaaaa cgcaaacacc
     3901 gtttttgcat tcggctatcg tcaggttgca ggactcaatc aggatccagt cttgattcgg
     3961 tttgattccg gcaaacgtac ctattgtaga actgattacg aaacgactca agcagaaggg
     4021 cggatctacg gcttgcttct ggccggtgag gatttgtacg ctgtcgcatc tcttgtagga
     4081 cctccaggga aagcagatca agactttcgc cggtttgcca ggaaaggatg gatgcgggat
     4141 tcaggaaaag gatctcccag ggcagccatc attctgaaaa tcaaagcaca gtcaggcgac
     4201 gtggaatctg cggcctattt gacttctgtt aaggaagatg ggaatagcaa ctcagtcgaa
     4261 gtcctggaaa tggatctgaa ctcaaaagga ctgacggtca aagcaaatgc ctggctttcc
     4321 cctcgtatga ccagtcgcgc tcccatgcaa tgcaagggta aagaacctta tttgcagact
     4381 atcattttca cgcctgacct ctcgaagaca gtggattctt tcgccgaacg atgcgtttgc
     4441 tgtgaatgat ttacacggac tccggagaaa tgctggaaag taaatggcac gcccgcgtat
     4501 ttgccctatg attcgcgcaa agaaatacgg cacgattgca gccattgcaa ttgcggcttt
     4561 actcattcta aagttcatct tactggatcg ctcacctgtt ccagagacat cgcccgaggc
     4621 cgtgaatttg aaactggttc gagctctggc aagggcttcg agaggagaat tgcctcagag
     4681 cctccacgta ctcatcatcg cggaaggcgc gtttccaaaa tctgcagtca tcgctggaga
     4741 aggactcttc aaaaagaaac caatggtctt cgattctttt caggctgtat tcgcggataa
     4801 gaaaacggtg atcattgata ctgccatgga cgcggctgga gttgagaaga tgtccaaagg
     4861 gatgcctttt tttgcggacc gttatgcggc aatgcaaaca gctatgacaa aagctgagcg
     4921 gatcattcta acgcatgagc atccggatca tatgggtggc attggcaaga ctgaggcaat
     4981 ggacaagatc aaagatcgtc tacttcttac caaagagcaa attgacgatc catacagaga
     5041 taagagttat ttcacagagg ataacattcg tgcgatttct tccgtgaagc ctcttgagta
     5101 tgatccttat ttttctcctg cacctggcat cgtgctcatt cgcgccgcag gacacagcgc
     5161 gggtagccag atgatttatg tcgctctcaa gaacggggaa gagtatctat tcgtgggcga
     5221 tattgtatgg tccatggaga atctggaaaa gctcaagggg aagccatact tgatgaatgc
     5281 agccggtttc ttaaaagaag atcgtgcgaa agtggccgcg caaatccgat ttcttcataa
     5341 tctgaccgcg gatcccgaaa acaaggtcca cgtggtagtg gcgcacgatt tagaacaaat
     5401 caaagattat atcgctagag gaatcgtcgc cgaaggcttt caatagaacc cgtttttggg
     5461 ttgccagaca acccttgaac cctaaactcc gggcagtgtt cgcaaaacac tgttcggggg
     5521 ggacatgcgt actttcaaat tctcaattgt tatagtttct atactagtgg cgctcactgc
     5581 ctgtggtggg aagaagagta agaaggggtt cttgcttttt ccattcgggg cgggaatggc
     5641 agtgctcgac gcaaatggga atgctcttcc agaaaactct ggaacctcgc agcaagaggc
     5701 tcccacagcc ggtccggctc agatttctgg ttccatttct cccatgaatg gttcatctgc
     5761 acagacgggg tttgatttct caggaattga agtttctctg gtggatcctc tggggaatgt
     5821 gatcgctacg gccactccag atagttttgg ggtttacacc ttcgatgtgc ctgatctttc
     5881 caataataat tatcgcgtac tgattcctga tggcggtgga ctaaaccagg gttacgtgga
     5941 ttttaatttt gtattcaacc cttctcagtc aggagcaacg ttagtcgctc ttcagccaat
     6001 catcacacaa gtgcagcagt ttgcggcagg gcctgcgaaa atttcaggga aggcgctcac
     6061 tccggggtac agcgacggag tgacaactgt gagcgcgggt ccgcttcctg ccgggactac
     6121 agtgcaactt ctggacgcag atgatgctgt catcgcaagc acaaccacga atgcatccgg
     6181 ggactattcg ttcaacattg caaatttaca caacggaaac tattctgtca aagtaatcgg
     6241 atcggcacaa tccgccagcg gtcgtccatt tactgatgga tctgtcgggt ttcattttgc
     6301 cttcgaaggt tacaacattg gaacagccac gcaggtgaat ctttctcccg cccagtgcgc
     6361 gtggactcct gcaacgcagg caccagcatc catcagtggg caggtagaca atgcggctaa
     6421 cgtggcggct gcatccggtc tgactgtgaa gctcatttct tctggcggtt ctgtggtggc
     6481 gcaaactact acagacggaa gcggggcttt cactctgtct gcatcttctg ttgccggcgg
     6541 catttatcga attgagatct ccggctccgc ctacaatact cagaatgctt ctttgctctt
     6601 tgttcctctc gcaaccggag catctacgag tgtgaatgct ggatccatat ctgtgattcc
     6661 taaggcttct aacattgccg gaaccattcg ggatgcggct acaagcgcgg gcattgcagg
     6721 tgccgtcatt agctttagac cggccgcatc tcagcctgtt tctaagctca cctatcttct
     6781 tacagacccg gtgctcggtg cggcagccgc aagatggatt gcagaaaaaa acggcacggg
     6841 aagctattcg acttacctca caaagtcgta taacgggcct gatgcgggtg gaaatctata
     6901 catgactgcg gtggccggaa agtgggctta ctatgtaagc gcggccggat atcagtcttc
     6961 tacagccgga gaaatcaccc tcaatggtgc ggacgagacg gcgaacattt cccttacatc
     7021 ggaccccaag agatcccgta tatctggtca ggcggt
//
LOCUS       DAORFF010000141         7041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5445_length_7041_cov_3.278414, whole genome shotgun sequence.
ACCESSION   DAORFF010000141 DAORFF010000000
VERSION     DAORFF010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5445_length_7041_cov_3.278414"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKK76_07410"
     CDS             complement(<1..240)
                     /locus_tag="PKK76_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNL68336.1"
                     /translation="MLCAADTVGLVKISIFQKSIHEPLGPSTFKKLTGLKSDFLVLPE
                     YLFADASVKDHASGIEKSKQALDWLLKLSDSYKGII"
     gene            338..925
                     /locus_tag="PKK76_07415"
     CDS             338..925
                     /locus_tag="PKK76_07415"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL68337.1"
                     /translation="MDLGRRGCQGTPRDFAAWVHFETGVEDPSVPAVLDNLVSAGKFK
                     HHPGFDHEYILASPIEFQTSVRYKVSGELKQSPIQMVRSCMEAFLRSQGTADETLMDI
                     SIATTEAMENAVKYSDHSPIEVSYSVENGVFHIMIKNRLSEVRLEKDIEAGKYTSTTT
                     LMRGMMVMVKLFDEMDIEILEEEKLAVFQAHKRIK"
     gene            1024..1872
                     /locus_tag="PKK76_07420"
     CDS             1024..1872
                     /locus_tag="PKK76_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68338.1"
                     /translation="MRKIALLAFVGLLAGDLSAASYKLFVHGRSGSNHCQALTSTASG
                     TTDYNNYWGGAVTGLTNVRYVGFDGTQSGGAYSWNNCGAQKQLNDAIRIFCANGNSCE
                     IYTHSTGGLVAAAYFALNNPSGISVTRIQLLASAAGGSELADFSTSYLSWLGFDTLGG
                     QLDQSVSTSGARNGFNHNTSNGRTYYTTSGEGTDYLYATSPLLPGYDDGVLANHSLCS
                     VNRVTAVGTSCTRGSARMTESYKCGFLWLSTCYYNYDRWTPYYTVWQGGSSESHGKAK
                     YDYNRR"
     gene            1965..2393
                     /locus_tag="PKK76_07425"
     CDS             1965..2393
                     /locus_tag="PKK76_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNL68339.1"
                     /translation="MSSRFAVAIHILTVLGLCTDKPVSSELIASSVNTNAVHIRRILS
                     NLKQAGLIRTTRGSQGGYLLARSTGEISLRQVHCALGENLFHFPRSTPNKECPAGANI
                     RSSLETVFGGATEAAHAHLDQTRLAHLVDEIRERSGQKGA"
     gene            complement(2491..2694)
                     /gene="rpmB"
                     /locus_tag="PKK76_07430"
                     /pseudo
     CDS             complement(2491..2694)
                     /gene="rpmB"
                     /locus_tag="PKK76_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017809470.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
     gene            2748..2963
                     /locus_tag="PKK76_07435"
     CDS             2748..2963
                     /locus_tag="PKK76_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HNL68340.1"
                     /translation="MAILKDIEKGAGEIIRVEVSEYQGKKYLNVRVWYTDKTSGEFKP
                     TQKGVAIRPDQFDEFLSAINAARAELQ"
     gene            2960..4120
                     /locus_tag="PKK76_07440"
     CDS             2960..4120
                     /locus_tag="PKK76_07440"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNL68341.1"
                     /translation="MKRRILLGLFGLSYLGLALWAFPDIRQTIRLHVLSWFGAASTGG
                     PTIAEDIFLSQFRPDPMLKTRQTILSHPFIPTVEFHGHLFTSPTSDPPEDILTRMDQT
                     HTSVFVNLSLFTSTPHSYQKLVEKQPSKRIFHFVGFNWKRMSEGPDFAQKMASDLEQI
                     AKMGGRGVKLWKNFGLMQKYEGKLVTLDDPRLDPVWDVCAKYHLLVAVHTADPPVFFL
                     PVDEHNERFEELGRRPEWSFHKPGLPSFDDVMQQRDRLFTRRKDVTFVALHFGEFAHD
                     LSRAQDLLDRHPNVYVDIAQRIDELGRQPRAAREFLIKNQNRILYGTDGLPDPEKLKI
                     YWRFLETDDEYFDYFPAGTNRKGVWKIYGVKLPPDVLRKIYYSNAARLLRIQ"
     gene            4257..6662
                     /locus_tag="PKK76_07445"
     CDS             4257..6662
                     /locus_tag="PKK76_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNL68342.1"
                     /translation="MLDRQGNASAPRPAQDVVYPEPPTTKKKIKTRGILDGPLFWTRG
                     SKSTGASRRFSTWSLPELSGRTLILGGAFLGLIPIVMFLALQIVPSVFLPEGGDTYLS
                     GLTTIRENRILDRKGRLVGELFSRKTGNLKYSEIPGSFRDILLFVEDEHFYSHGGVHW
                     PSVVRAFARNVTSMGYSQGGSTLTQQLARILLNARQKTLLRKVRETSLAYYIEGHMSK
                     EDIFTAYVNQVYLGHGAVGADNAARFYFLKGIADLNFAQMLALSCLPSAPEMYSPLRY
                     PDRIERKMDAVFARMKSEGFSGAAEITQEQYESQKAAMLSNTTRSPSETVFGNREDIA
                     PYVSEHVRNKIGELFGVEQEFGAGLQIETTIDADLQNSANTSTAEFLASAQGNFPPVR
                     KGNPGSSEDALKRAIVDEYFHMGLGAVMLGMPSSSQSEHTSETVLQGASVGIDPRTGA
                     VLYMQGGRSFRRDNQINRAVSMRRQTGSAIKPIIYSAGIETGEITAASRLDDSPVFVF
                     HGGQGPREFWLPENITGEYKGKISAREALAQSRNVPAILVGRQVGLPRLSGQFRKFFF
                     PDDSTFDRRFRMDETIAIGSLEMSPLEMAVAFAAFADNGTIHRPYLILRIKDTSGKIL
                     YENDGSQDEFGTHLPLMSRVLPGDVAEVMSSMLRDSAAFANTGVFSGLLMGKTGTTNE
                     YRDAWFVGAIPGLSASAWVGYDNPTYSMQGGMGARLAGPLFGRIVSRSGFSPNGSYAF
                     SPSAERIRICKESGKISGPKCPSVTELFASVRRPEGICDLHDAGGNRTDRKDELSRQS
                     DFE"
     gene            6675..6986
                     /locus_tag="PKK76_07450"
     CDS             6675..6986
                     /locus_tag="PKK76_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68343.1"
                     /translation="MEAKKVDHRRLSQVISQMKKDPEGSMGIYVVGGWRVQISRYKQT
                     SAERVRALYSRRRDQGLCIACGKKVTKTNPANGRLYRLCEDHRKNIDYKKAGKPAKRR
                     K"
BASE COUNT         1774 a         1728 c         1821 g         1718 t
ORIGIN      
        1 gatgatccct ttgtaggaat cgctcagctt gaggagccag tccagggctt gtttgctttt
       61 ctcaatgccg ctggcatggt ctttcacgga tgcgtctgcg aaaaggtatt cgggcaaaac
      121 caggaaatct gatttcaacc cggtcaactt cttgaatgta gaaggcccga gtggttcgtg
      181 aatggacttc tggaagatcg atattttaac caggcccaca gtatctgctg cgcaaagcat
      241 gacgcgctgt caagaacgat ctttattact tgcaatcctg gacaagattc tataaatcct
      301 ctattcaggc gaagtggcga gcaccgagat aacattgatg gatcttggcc gccgtggctg
      361 ccaggggaca ccgagggatt ttgcggcatg ggttcatttt gaaaccggcg tagaggatcc
      421 gtccgttcca gcggttctgg acaacctggt cagtgccggg aaattcaaac atcatccggg
      481 ctttgatcac gagtacatac ttgcatctcc tatagaattc caaacgtcgg tgcgctacaa
      541 agtatccggg gaattaaagc aatctcccat tcagatggtg cgatcttgta tggaggcatt
      601 tctaagatcg caaggcactg cggacgagac attgatggac atcagtatcg ctacaacgga
      661 agcaatggag aacgctgtaa aatacagtga ccacagcccc attgaagtct cctattcagt
      721 cgaaaacgga gtattccaca taatgatcaa gaaccgcttg agtgaagtga gactggagaa
      781 ggacatagaa gcgggcaaat acaccagtac aaccacactc atgcgcggaa tgatggtgat
      841 ggtgaagctg tttgatgaaa tggatattga gattctcgag gaagagaaac tggccgtttt
      901 tcaagctcat aaacgaatta agtagacgta ccgttttttt gccgataggt tcaaaaatga
      961 gttgcacgta cacgaatcta tggcacggtg caccataaac tactactacg ctagggggat
     1021 aacatgcgta aaatagcttt gttagcgttc gtaggattgc ttgctggaga cctttcagct
     1081 gcttcttaca aattgttcgt tcatggccgc tctggatcaa accactgcca ggctcttact
     1141 tctactgcaa gcggaactac tgactacaac aactactggg gcggagccgt aactggtctg
     1201 accaatgttc gttacgtagg ctttgacgga actcagagcg gtggtgcata cagctggaat
     1261 aactgtggtg cacaaaagca gctcaacgat gcgattcgca tcttctgcgc aaacggaaac
     1321 agctgcgaga tttacactca ctctactggt ggactggttg cagcagctta cttcgcactc
     1381 aacaatccat ctggaatcag cgtaaccaga atccagcttc ttgcatccgc agcgggcggt
     1441 tctgaactgg ctgacttctc cacttcttac ctttcctggc ttggattcga cactctgggc
     1501 ggtcaactcg accagtctgt gagcacaagc ggagcgcgta acggattcaa ccacaacact
     1561 tctaacggcc gcacttacta cactacttct ggcgaaggca cagattacct gtatgctacg
     1621 agcccactgc ttccaggtta tgacgatggc gttctggcta accacagcct ttgcagcgta
     1681 aaccgcgtaa ctgcagttgg aacttcctgc actagaggat ctgctcgtat gactgaatcc
     1741 tacaagtgcg gattcctgtg gctgagcact tgctactaca actacgaccg ctggactcca
     1801 tactacactg tatggcaggg cggaagcagc gaatcgcacg gcaaagccaa gtacgactac
     1861 aatcgccgct gattctaatt caaagagatc gagaaaaaag ccgggaattt cctcccggct
     1921 tttttttgct ttgagtgcga gtgtcgcttc caaacctttg acaaatgtcg tcgcgttttg
     1981 ctgttgctat tcatatcctg accgttctgg gtctttgcac ggacaaaccc gtttcctctg
     2041 agctgattgc ttcgagcgta aatacaaacg ctgtccatat tcgacgcata ctctcgaacc
     2101 tcaagcaggc tggtctcatt cggaccactc ggggcagtca gggtggatac ctcctggcca
     2161 ggtccacggg agaaatctcg ttgaggcagg tgcactgtgc tctcggagag aatctgtttc
     2221 actttccccg cagtactcca aacaaagaat gcccggcggg cgccaacatt cgatcatcgc
     2281 tggaaacagt atttggggga gctacagagg cagcccatgc acatctagac cagaccaggc
     2341 tggctcattt agtggacgaa atcagggaaa gaagcggtca gaaaggcgcc tgatcttaga
     2401 tcaggctgct gaaccttttg cctggggcgc tggcttggct gaaagtgctt tggcagcttt
     2461 caaaacggac ggatccattt tatactttga aatgatggcc ttcgggcctt tcttgttcag
     2521 agtgcggagc attcttgcgg aaatacgcag gcgcacccag cgattttcgt cttcgaggaa
     2581 gacgcggcgc tctaccaggt tgatctggaa tttgcgtttc gtgactctgt gggagtggga
     2641 aacctgagct ccaaaaccag attgtttacc ggacaattga catctgcgcg ccatttggcc
     2701 aggatttcgc tggaaggacg atcggtcaat tcaaaagggt ggtaatcatg gcaatcttga
     2761 aagatattga aaaaggcgcc ggagagatca ttcgggtgga agtatccgaa taccagggaa
     2821 agaagtatct aaacgtgcgg gtctggtata cggataaaac ttccggtgaa ttcaagccca
     2881 cccagaaggg tgttgcgatt cggccggatc agttcgatga gtttttgtcg gccataaacg
     2941 ctgcacgtgc ggaactacaa tgaaacgccg cattctgctc gggttgtttg gactgagtta
     3001 cctggggctc gctctgtggg cgtttcccga cattcgtcaa accattcggt tgcatgttct
     3061 gtcctggttt ggagccgctt ccacaggcgg tcccaccatt gcggaagaca ttttcctttc
     3121 tcagtttcgt ccggatccga tgctcaagac tcgtcagacg attctctctc accctttcat
     3181 cccgactgtc gagtttcacg gacatctttt taccagcccg acaagtgatc ctccagagga
     3241 cattctcact cgtatggatc aaactcacac atctgtgttt gtgaacctgt ctctgtttac
     3301 atccacgccc cactcctatc agaaacttgt agaaaaacaa ccatccaaaa ggatctttca
     3361 tttcgtcgga ttcaattgga agagaatgag cgaaggtccc gattttgcac agaagatggc
     3421 gtctgatctc gaacagattg cgaagatggg tggccgcggc gtgaagctat ggaaaaattt
     3481 cgggctcatg cagaaatacg aaggtaaact ggtgacgctg gatgatccaa ggctcgaccc
     3541 ggtctgggac gtctgcgcga agtatcatct gctcgtcgca gttcatactg cggatccacc
     3601 tgttttcttt ctgcccgtgg acgagcacaa tgaacgtttc gaagaacttg gccgaagacc
     3661 agaatggtct tttcacaagc ccgggctgcc ctcattcgat gacgtgatgc agcagagaga
     3721 tagactgttc accagacgaa aggatgtcac ttttgtggca cttcactttg gagaattcgc
     3781 gcacgatctg tcgcgagcgc aagatttgct ggatcgccat ccgaatgtct acgtggacat
     3841 cgcgcagaga attgatgagc tcgggcgtca acccagggcc gctagagagt ttctgatcaa
     3901 gaaccagaat cgcattttgt acgggacaga tggtcttcct gatccagaaa agttgaagat
     3961 ttactggcgt tttctcgaaa cagacgacga gtatttcgat tactttcctg ccggaaccaa
     4021 ccgaaaagga gtttggaaga tctacggtgt aaaattgccc ccggacgtgc ttcgaaagat
     4081 ctactatagc aatgcagccc gcctcctcag aatccagtga actgaaaaaa ttcgtacatg
     4141 tctgccctgc ctgccagaac agataccgcg ttacgattca ggtaaaagta tacaaggttc
     4201 acagggcggc atgccctgta tgtggaaacg taaactattt tgacaatcgg agaggtttgc
     4261 tagacagaca ggggaacgca agtgcgccca gaccagcgca ggacgtcgtc tatccagaac
     4321 cgccaaccac aaagaagaaa atcaaaaccc gcgggattct agacggtcct cttttctgga
     4381 cgcgcggttc taaatcaacg ggtgcatcgc gaagattcag cacatggtct cttcccgaac
     4441 taagcgggcg gacattgatc ctcggcgggg cgtttctggg attgattccg atcgtgatgt
     4501 tccttgcgct gcagattgtt ccttccgtct ttctccccga aggcggagac acatacctgt
     4561 ccggccttac taccattcgc gaaaacagaa ttcttgatcg caaaggccgg ctcgtagggg
     4621 aattgttttc gcgaaagacg ggcaatctta agtattctga aatcccggga tcttttcgcg
     4681 acatacttct ctttgtagaa gatgagcatt tctattcgca cggcggcgtg cattggcctt
     4741 ccgttgttcg agcatttgcg cgtaacgtta cttctatggg gtattctcaa ggaggctcta
     4801 ctctaacgca acagctagcg cgaattcttt tgaatgcgcg acagaaaact ctgctacgca
     4861 aagtgcgcga aacatctctc gcctactaca tagaaggcca catgtccaaa gaggacattt
     4921 ttactgcata cgtgaatcaa gtgtacctgg gacacggtgc cgttggtgcg gacaacgccg
     4981 ctcgttttta tttcttgaaa ggaatcgcgg acttgaattt cgcgcagatg cttgcgctca
     5041 gctgtcttcc ttctgcaccc gagatgtatt cgcctctgcg atatccagat cgaattgaac
     5101 ggaaaatgga cgctgtcttc gcacgcatga aatcagaagg attttcgggt gcggcagaaa
     5161 tcactcagga gcagtatgag tcgcaaaaag cggccatgct ttccaacacc actcgcagtc
     5221 cgtctgaaac agtatttgga aatcgggagg acatcgctcc atatgtatca gagcacgtgc
     5281 gaaacaagat tggggaacta ttcggtgtgg aacaagaatt tggggccggt ctgcaaatcg
     5341 aaactaccat agacgcggat ttgcagaact cagcaaacac atccaccgcc gaattcttgg
     5401 cgagtgctca ggggaatttt ccccctgttc gcaaagggaa tccaggctcc tctgaggatg
     5461 ctcttaagcg agcgattgtt gacgaatact ttcatatggg actgggtgcg gtgatgctcg
     5521 gcatgccttc ctccagccag tcagagcata cttcggagac cgttttacag ggcgcaagcg
     5581 tgggaatcga tccgcgcaca ggtgcagttc tctatatgca gggtgggcga agctttcgcc
     5641 gcgacaatca gatcaatcga gccgtttcca tgcgtcgtca gacaggaagc gcgatcaagc
     5701 caatcatcta ttccgccggc attgaaacag gcgaaatcac ggccgccagt cgtctcgacg
     5761 attctcctgt gtttgtcttt cacggagggc aggggccgcg cgaattctgg cttcccgaaa
     5821 acatcactgg tgagtacaaa ggaaagattt ccgcacgtga agcactggct caatcgcgta
     5881 acgtccccgc tatactcgtg ggccgtcagg tgggattgcc ccgtttgtcc ggacagtttc
     5941 gaaaattctt ttttccagac gacagtacct ttgacagacg atttcgtatg gatgaaacga
     6001 ttgcgattgg ttctctggag atgtctcctc ttgaaatggc ggtcgccttc gcggcgttcg
     6061 cggataacgg aacgattcac aggccctact tgatactgag aatcaaagac acatcaggaa
     6121 aaatacttta tgaaaatgat ggatcgcagg acgagtttgg tacacatcta ccattaatgt
     6181 ctcgtgttct tcctggagat gtagctgaag tgatgtcgag tatgttgcgg gattccgctg
     6241 cattcgcaaa cactggtgta ttctccgggt tgctcatggg aaaaactgga acgacgaacg
     6301 aatacagaga tgcatggttt gtcggcgcga ttcctggttt gtctgcatcc gcatgggttg
     6361 gatatgataa tccaacctac tcgatgcaag gtggaatggg tgcgcgcctg gctggccctt
     6421 tgttcggacg aatcgtttca cggtcagggt tttcgcctaa tggcagctat gcgttctctc
     6481 catctgcgga gcgaattcgt atttgcaaag agtctggaaa aatttccgga ccgaagtgcc
     6541 cgtccgtgac cgaattgttt gcgtctgttc gtagaccaga agggatatgc gacctgcacg
     6601 atgcaggcgg taaccgcacc gatcgtaagg atgaactgag ccgtcagtcg gatttcgaat
     6661 aaaggaccac aaacatggaa gcaaaaaaag ttgatcaccg tcgtctctcg caggtgattt
     6721 ctcaaatgaa gaaagatcca gaaggcagca tggggatcta tgtcgtcggt ggatggcgcg
     6781 ttcaaatcag ccgttacaaa caaaccagcg ctgaacgggt gcgtgctctg tacagccgca
     6841 gaagagatca gggactttgc attgcctgtg gcaaaaaagt taccaaaacc aatccggcta
     6901 atggtcgact gtaccgcctc tgcgaagatc atcgcaagaa tattgactac aagaaagcag
     6961 ggaagccggc aaagcgccgg aaataggtta ctgcaagcaa cggtcctact gtgcactctg
     7021 gtaacacaga atgcatatgg c
//
LOCUS       DAORFF010000142         7018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5472_length_7018_cov_3.781129, whole genome shotgun sequence.
ACCESSION   DAORFF010000142 DAORFF010000000
VERSION     DAORFF010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5472_length_7018_cov_3.781129"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1070)
                     /locus_tag="PKK76_07455"
     CDS             complement(219..1070)
                     /locus_tag="PKK76_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68344.1"
                     /translation="MKLLFLILTWLHRQWLFHCRSFRTGKNRSDQYIRRSGTCACGDC
                     ELFSNSCGAIYAPYKEANGLRRLDEMDWTLGYERQTKIGTMGGGLLVQTMSDATGKAN
                     PFISGGSGYILTEFFLTYALPQFKDLKLYAGADVIRSDQYYKLTYSHEFPINDSMSVS
                     VNTGPGYMVTQNLQGVRDVPTTVQLNVKGFNVGFNAVYRPKLEFFDTDTATTRAVWLD
                     GGSTKADGLVADPSKYQAGVVSDAINGAITQAVRTRSGNTNYNYVPRQKLPHWLYFVN
                     LGYTSTF"
     gene            complement(982..1500)
                     /locus_tag="PKK76_07460"
     CDS             complement(982..1500)
                     /locus_tag="PKK76_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68345.1"
                     /translation="MKMPQTKILLVTLALNGALFAQEVPRDPATENKPTTEPAKQADP
                     AQTNTQTPSSQTPALPDASIQVSLQLVSNSVFRGDDTFKGRAQQKRKQYGTHSGEWMF
                     QPSITFNTPLEGLTFNWVGSFALAGRQDTDVDQRIESGRDGNETTFFDSYMAPSPVAL
                     PLPQLSHRQEQI"
     gene            1608..3011
                     /locus_tag="PKK76_07465"
     CDS             1608..3011
                     /locus_tag="PKK76_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68346.1"
                     /translation="MNRKPLFGLKLDLLQGAFARLSKEALAIRYGIRPNRPIELSWKE
                     SPHQPGLRIIEATAGPKVDAGLIVGSVRMGYGHHRMAYSVSSWAIARGETPYLHDLLA
                     IDSPESKLIRESDDLYSFLSRLASEWGGPVEWAWGMVTMNGDLSSLQFSTLLAEGLRN
                     LITDIKPILPLVSSYPLNGQMAVAAGFRRVVHLVPDNHPQYYLLVPGALNLVQSNGAY
                     AKFIQMGVPAENLAVAGHWVSHDIAVNAEADSRVRMERLDAKKPVRVLFSVGGAGAQK
                     SYLSRFIQAIVDLIRSGQVHLWVNAGDHAEVADAVRTAFDKLKIGYSTVESFSDLQTF
                     CSAYPLHGDDPARNAVVFLFSSHYEAFTATDLLIRRCDLLSTKPGELAFFPVPKLFAK
                     RVGDHEAASAVRSMELGEGTAECRSPSDAAALLRKMISDRDLLRQMSEAVIQNAADGI
                     YNGSKTAVDIALSMKDE"
     gene            3286..3972
                     /locus_tag="PKK76_07470"
     CDS             3286..3972
                     /locus_tag="PKK76_07470"
                     /inference="COORDINATES: protein motif:HMM:NF025021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNL68347.1"
                     /translation="MLLDKRNSLIFLIILFVSLCTRVDGDPAYDRAQQQRQQMRDYNN
                     QQRWNSANTELQRSYERAQENARQESMRRTTNTSPTTTNPGLNAGKSNPASRPEPGQK
                     LDEPAIREQLRRQYLLQTRFETAIKSDDVDEVRSIIKSGAYADWPSDVEEPALFLAAG
                     ASYRLFAGKPGDKCATAANQPHPEILRVLLEAKVNPNQKDLYGNTALFQVSARGYLEV
                     AQMLIRSGPT"
     gene            3969..4589
                     /locus_tag="PKK76_07475"
     CDS             3969..4589
                     /locus_tag="PKK76_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNL68348.1"
                     /translation="MNAQNDKGESVLYYLASNRCTVGVDAVRLLIASKADVNATSRLP
                     YHTLGECVQAGIHQMLCEGYLASSGRTPLLEAAAHGTEDFVSVLVEAHADINAQDPKN
                     GKTPLMEAVEHGNSGAVKVLIDAHADLNARARFGETALFFARSNANVTRLLIAAKADL
                     NARDQWGHTALYWAKKRSCEIDLFGLCLYHGADEVADLLRASGAED"
     gene            complement(5762..6214)
                     /locus_tag="PKK76_07480"
     CDS             complement(5762..6214)
                     /locus_tag="PKK76_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68349.1"
                     /translation="MQKRTSYAISVLLAFWMPAQILSERPKQSTEEVVKEALAAIEKG
                     DGELIEGMRDKIKTQHLAPIAATWKASFPWSKKDWYIALLMDHQDPILTPMMEDALDS
                     PTAESRAYALMILKKDFSLHKDLWTKSGWIIPAKVDEAVKKYRAEKKK"
     gene            6252..6965
                     /locus_tag="PKK76_07485"
     CDS             6252..6965
                     /locus_tag="PKK76_07485"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HNL68350.1"
                     /translation="MRPTGTVIRSFFNWSGGKDSALALYHCKQNPEVDIAHLVTSVNA
                     HHGRISMHGVRESLLDAQAEALGIPMRKLYLPESPDMKEYESLMSRLVTDLVADQCTH
                     AIFGDIFLEDLRRYREEKLATQGLQGLFPLWKRDTSELIREFLDLGFKTMLVCVDSSL
                     LDQSFAGRIIDSDFLNDLPENVDPCGENGEFHTFVFDGPIFQSPIPVAKREVVYREYP
                     APQGGKPIGFYFQDIETVP"
BASE COUNT         1637 a         1697 c         1908 g         1776 t
ORIGIN      
        1 ccatcatgcc atctgtctag agtgtggagc ggcgttttgt attgatgttt gttttgtgcc
       61 agatcgaatc ccggctccca aaaaagacaa aggattcaaa gtcacttcgc acgcatttga
      121 ggtttatggt acgtgctcaa aatgcgcggc atgacgcttg ctgcgaagca atgcgatccg
      181 gttttatccg gatcgtctaa aatgttcaga ttttggttct agaaagtgga agtatacccc
      241 aggtttacaa agtacaacca gtgtggaagc ttctggcgcg gcacgtagtt gtagtttgta
      301 tttcctgatc gtgtacgtac cgcctgcgtg atggctccgt tgattgcatc ggatacaaca
      361 cctgcctggt atttggatgg atctgccaca aggccgtctg ctttggtcga tccaccatca
      421 agccacactg cacgagtggt tgcggtgtcc gtgtcaaaaa attcaagctt tggtctgtag
      481 actgcgttga atccaacgtt gaatcctttg acgttcagtt gaacagtcgt aggtacgtcg
      541 cgaaccccct gcagattctg cgtgaccatg tagccaggac cagtgtttac agaaacggac
      601 atggagtcat tgatcggaaa ttcgtgcgaa tacgttagct tgtagtactg atctgatctg
      661 atcacatcgg caccagcata tagtttcaaa tctttgaatt gaggtagggc gtacgtgagg
      721 aagaattcag tgagtatgta tccactgcca ccactgatga atggatttgc tttccccgtg
      781 gcgtctgaca tggtctgcac gagtaggcct ccgcccatgg tcccaatttt cgtctggcgc
      841 tcatatccga gagtccagtc catttcatcc aatctgcgaa gaccgtttgc ttctttgtaa
      901 ggcgcataga tcgcaccgca cgagttggaa aagagttcgc agtcgccgca ggcacaggtc
      961 ccactccgcc gaatgtattg atcagatctg ttcttgcctg tgcgaaagct gcggcagtgg
     1021 aagagccact ggcgatggag ccatgtaaga atcaaaaaaa gtagtttcat ttccgtctct
     1081 gccggattcg attctctggt caacgtccgt gtcctgtcgt ccagccagag caaagcttcc
     1141 tacccagttg aatgtgagcc cttctagagg ggtgttgaaa gtaatggaag gttggaacat
     1201 ccattcgcct gaatgagtgc cgtactgctt gcgcttttgt tgcgcacggc ctttgaatgt
     1261 gtcgtctccg cgaaagacgc tgtttgaaac cagttgcaga ctgacctgaa tgctggcatc
     1321 cggcagagcg ggtgtctgcg aagaaggcgt ttgtgtattt gtctgcgccg gatctgcctg
     1381 ttttgcaggc tctgtggttg gtttgttttc tgttgccgga tctctgggca cttcctgggc
     1441 gaaaagtgca ccgttcaggg caagcgtgac caataggatc ttcgtttggg gcatcttcat
     1501 aaagttggcc tgatttatcg gttgcaaccg taaaagcaac ttgtaaatcc aacagagtcg
     1561 aatctggaga aatagttgag actgtatctc aatatgggca atcactgatg aatcgtaaac
     1621 cattgttcgg gctgaaactc gatttgcttc agggtgcttt cgcacgactg agcaaggagg
     1681 cactggcgat tcgatatgga atcagaccga atcgccccat cgagctttca tggaaggaat
     1741 cgcctcacca gccaggtctt cgcatcatag aagcgactgc aggtccgaag gtggatgcag
     1801 gtctgatcgt aggaagcgtt cgcatgggct acggccatca tcgcatggct tattccgttt
     1861 ccagctgggc catcgcacgc ggagaaactc cctaccttca cgatctcctg gcaatcgatt
     1921 cgcctgaaag taaattgatt cgcgaatcag acgatcttta tagttttcta agcaggctcg
     1981 catctgaatg gggcggcccc gtggagtggg catggggaat ggtcactatg aacggtgatc
     2041 tgtcctcgct tcagttctcc acattacttg cggagggtct tcgcaatttg attacagata
     2101 ttaaaccaat ccttccgctt gtgtcttctt atccattgaa tggtcagatg gccgtggcag
     2161 ccgggtttcg cagagtggtg catcttgtgc cggacaatca tcctcagtac tacttactgg
     2221 ttccgggagc tttgaatctt gtgcagagta acggtgcgta tgcgaaattc attcagatgg
     2281 gcgtccccgc ggaaaacctg gcagtggcgg gacactgggt ttcgcatgac atcgcagtga
     2341 atgcagaggc agactcaaga gtgcgcatgg aaagactgga cgcgaagaag ccagtgcgcg
     2401 tgcttttttc tgttggcggc gcgggggcgc aaaagagtta tctttcgcga ttcatccagg
     2461 cgattgtgga tctgatccga tccgggcagg ttcatctctg ggtgaatgca ggagatcatg
     2521 ctgaagtggc agatgcagtt cgcactgctt ttgataaatt gaagattggt tattcgaccg
     2581 tggagtcctt ttcggatttg cagacattct gtagcgcgta ccctttgcat ggagacgatc
     2641 cggcgcgaaa tgcagtggtg tttctttttt cttcgcacta tgaggcattc actgcaacgg
     2701 atctcttgat ccgtcgttgt gatcttctct ctacgaaacc aggggaactt gcgtttttcc
     2761 ccgttcccaa gttattcgca aagagagtgg gggatcacga agccgcttct gcagttcgct
     2821 cgatggagct gggagaagga acggcagaat gcaggagtcc gtctgatgca gccgcccttc
     2881 ttcgtaaaat gatttcagat cgtgatttgt tgcgtcagat gtcagaggca gtgattcaaa
     2941 atgcggcgga cggcatttac aatggcagca aaacagcagt ggatattgcg ctttcgatga
     3001 aagacgaatg atgtgcattc tgaaaccata acaggatctt ggaaagggag cgaaaagaac
     3061 ctggttttca agcaatgacc cgcgtctgaa tactctgaaa cctggttggc gccacactgc
     3121 gcttcaatga aaagacatgc catgctagga catacgcagc gctggggcct gtcaaacaca
     3181 cgactgccct gagcatactc catccgtgca tttattgcga gaggagccat cggatttaag
     3241 gcaaatcttc tttacacttg cagagcagaa tagtaagctc acgccatgct tctagataag
     3301 aggaattctc tcatctttct gatcattttg ttcgtttctt tgtgcactcg tgtggacggc
     3361 gatccggcat acgatcgtgc gcaacaacaa cgccagcaga tgcgggatta taacaatcag
     3421 caacggtgga atagcgcaaa tactgaacta cagcgctcgt acgaaagggc ccaggaaaat
     3481 gccagacagg agtccatgcg gcggaccacg aacacttcac ccacaacaac aaatccaggg
     3541 ctcaacgcag ggaagagtaa cccggcgagc cgccctgaac ccggacaaaa actcgatgaa
     3601 cctgcaatac gggagcagtt gaggcggcaa tatttgcttc aaacacggtt cgagactgcg
     3661 attaagagcg atgatgttga tgaggtcaga agcatcatca aatcgggcgc gtatgcggat
     3721 tggccgtccg atgttgaaga gccagctcta ttcctcgctg ccggggcttc gtatcgatta
     3781 ttcgccggta agccaggaga caaatgcgca acagcggcaa accaacctca cccggagatc
     3841 ttgcgggtcc tattggaggc caaggtcaac ccgaatcaaa aggatctgta cggcaatacg
     3901 gcgttgtttc aggtgtcggc gcgcggatat ttggaggttg cgcaaatgct cattcgttca
     3961 gggccgacgt gaatgctcag aacgacaagg gtgagtcagt tctatactat ctggctagta
     4021 atcgttgtac cgtgggtgtc gacgcggtgc gactgctcat tgcatcgaaa gcggatgtta
     4081 acgctaccag tcgcctacct taccatacgc tcggggagtg cgttcaagct ggcatacacc
     4141 agatgctctg tgaggggtat ctggcaagta gcgggcgcac gcccctgctg gaagcggccg
     4201 cgcacgggac agaagatttc gtcagcgttc tggtggaggc gcacgcggat attaatgctc
     4261 aggatccaaa aaatggcaaa accccactaa tggaagctgt tgagcacggg aactctggcg
     4321 ctgtaaaggt tctgatcgac gctcatgccg acctcaatgc acgggctcgt tttggagaaa
     4381 cagccctctt cttcgcgcgg agcaacgcaa atgttacgcg attgctgatc gcggcgaaag
     4441 cggacctcaa tgcgcgtgat caatggggcc ataccgcctt gtactgggcc aaaaagcgct
     4501 cttgcgaaat agacctattc ggcctttgtc tctatcacgg tgccgatgag gttgcggatt
     4561 tgctcagagc ctccggggca gaggactgac ctatgcgtta ccttagagta tacagccttt
     4621 ggactttctt ggtcttggtt gtgagctgcg attgcaggaa gtccccggaa ctgatgcccg
     4681 cccaggaact ttatcgggct tgcacaggtc agggaataag ctatacaact cttcagccag
     4741 aacgcgggct tgtgagatcg aaacatgtag aggattactt taaatgcaag gatcctgcag
     4801 agagcgcgcg ctgccctatt cgcgcggccg gtgaggagga gaacaatgat cgggcgtacc
     4861 gccaggcatt tgactacgtg aggacagcct atatttgcat tcaccttccc agtggccaaa
     4921 cgtccacgta cggtggtggg cgggcttccg tcactgtgaa cgctgcccac cagcaggtgc
     4981 tccgtattcc acagctcttg ttcctcacaa gagatcaaga gtggaactgg atcgcacgct
     5041 tgaaattcgc atcccttcga atgccacgct cgctgatctg gacatctaca ttttcgcgaa
     5101 gccgacgctt ccaaagaaca gtgcatcgtc actcgacgtc aagctttcgg atctaacggc
     5161 ctatgcagtg gtcaaacacg attgcgtttt cgaagctgaa caaaggcaca gtggaccctt
     5221 ccaattatcc tacgacgcgg ctagcaggtg cgcgaatcag ttactgttgt cggtgccgac
     5281 tggaaaaagc gcagatttga ccacagaagg actggagaat ctcctggagg acaactctcc
     5341 atcacggaag tagccgcgga ctcagaggaa cggattcgcg attgcggttt ggcacgtaac
     5401 ccaagccgaa tgaggacgtg aaagaatggg ctcgaagtat ttcagattcg ctttgcggaa
     5461 ggtttcataa cgtgagacgc acagcgggat ttgcttaacc tggatcactt catggcttct
     5521 ggtttctttg ctttgcacat gcgtggatcg cgggggaaca actgccgctg ctgcatttga
     5581 atgcctggat cgcgagaaat ctgtcttcaa ggtgaatctc ttgaactgtt agaggcatcg
     5641 aggagatttg agcgggggtt tgagagattt cagaaacgca gcggcacttt gcgcggtttt
     5701 tttagttttc aattgagctt tcgccatctt ttcgatcttg ccttcactgc acgcgaaggg
     5761 gttatttctt tttttctgcc cggtatttct tcacagcttc gtcgaccttc gcgggaataa
     5821 tccacccgct ctttgtccag agatccttgt ggaggctgaa gtcctttttg agaatcatca
     5881 gcgcgtatgc gcggctttct gccgtcggag aatcgagcgc gtcttccatc attggcgtga
     5941 gaatgggatc ctgatggtcc attagcaggg cgatgtacca gtcttttttc gaccagggga
     6001 aagatgcctt ccaggtggct gcaatgggcg caagatgctg cgttttgatt ttgtcccgca
     6061 ttccttcaat caattctcca tcacctttct cgatggctgc gagggcttct ttgactactt
     6121 cctctgtact ttgctttggt cgttccgata gtatctgcgc gggcatccag aaagcgagca
     6181 gaacagaaat cgcataagaa gttctttttt gcattcgcca gaaagtggaa gtttgttccg
     6241 actgtcaagc tatgagacct accgggactg tgattcgatc ctttttcaac tggagcggag
     6301 gcaaagacag cgctctggct ctctatcact gcaaacaaaa tccggaggtc gacatcgcgc
     6361 atctggtgac ctctgtgaac gcgcatcatg gtcgaatctc catgcacgga gtgagggaga
     6421 gcttgctgga cgcgcaggca gaggcgctcg ggattcccat gcgcaaactc tatcttcccg
     6481 agtctccaga catgaaggaa tacgaatcgt tgatgtcgcg tctggtaact gacctcgtcg
     6541 cagatcagtg cacgcatgcg attttcggag atatttttct agaagacctc aggcgctaca
     6601 gagaagagaa gctggcaacg caagggttgc agggtctgtt tcctctgtgg aaaagagata
     6661 cgtcggaact gattcgcgaa ttcctggacc tgggatttaa gaccatgctc gtatgcgtgg
     6721 attcatcgct actggatcag tcttttgcag ggagaatcat tgactctgat tttttgaacg
     6781 accttccgga gaatgtagat ccttgcggtg agaacggcga atttcacaca ttcgttttcg
     6841 acggtcccat tttccaatcg cccattcctg ttgcaaagag ggaagtcgtc tatcgtgagt
     6901 atcctgcacc tcagggaggg aagcccatag gattctactt ccaggacatt gagacggtcc
     6961 catgaggagg aggatcagaa atgaacgtta gacggcatat ctttcttttt tcccttac
//
LOCUS       DAORFF010000143         6933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5577_length_6933_cov_3.271881, whole genome shotgun sequence.
ACCESSION   DAORFF010000143 DAORFF010000000
VERSION     DAORFF010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5577_length_6933_cov_3.271881"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKK76_07490"
     CDS             <1..411
                     /locus_tag="PKK76_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68351.1"
                     /translation="SKTIFQPLLVINEDSEWSIKGLHSGHYKDLTLRKGESRTKIRAA
                     GFEAQSGSVYEMMIPLSDVQPRTNFRRVRIHFYLFAGQKHDSVVLDLRKDESGRWAAA
                     TSRMMPDAESRERPSVPTCMSASEDSCIESLHRQ"
     gene            451..2736
                     /locus_tag="PKK76_07495"
     CDS             451..2736
                     /locus_tag="PKK76_07495"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNL68352.1"
                     /translation="MPVFPLGVRNHTLWFGRTRRAFAWLCVLALVSCFAGCSSPSGPA
                     AAAGRIDLSSTDLTRATPLDGEWRFIWQKFVDPASQAEEGWSFQKVPGFWNSVPGLQA
                     EGFATYRLDVQLPPGQRLYGLRIVDMESSYRLFAGTREIAANGVPGISRETEIPEWKP
                     AVGLFEANGNVRLTLYVSNFHHRLGGIWQGIKIGNAETILSAQRKSLALDLAVTGSLI
                     IIAVYHALIFSVRRTERGFILLPIFCLIMALRTLVISERFIAVMFPGIPWDLLVRLDY
                     LSVSLGLISAHAYLKVVFARYYHRFVFVLLLAVFGPCAAAEAFLPVRWFTHLLPVHNL
                     FLLLFSLYFLVIAFQAVRERMPGAAAIWFGWLVAISANVNDSLYIMSAIDSAYLSAIG
                     LQAFILTQSYYLARRYSEAFALSAELARRMQSLLGLTREFNRAADRGTAVRRALREIS
                     DILEKPDLQAFIPVRDSPLMRKISADRPDEFADLPWSTKAESITSQVQEDGRVLIPLR
                     GGGELLCLIDLPGFEDARTQYVQGILDSLSLVLDNMRRVESVALASIGQAAAEIVHDI
                     NHHCQVIKAHASSGQHSVERLRLIENETGLMTNLALDILDFARENVIVNPMAVSLESV
                     KDAFNQDIGSAFMNTDMRHSIVVNAAGDIRIDLERFRRVCLNLARNAMEACNGSGLFR
                     VEIEIGSDTVIMVFEDDGPGIDPAMQEKLFEPFASGGRGTGLGLAVVRRIVSAHGGDI
                     RVYSTPGKGCRFVVMVPLNFG"
     gene            2768..3388
                     /locus_tag="PKK76_07500"
     CDS             2768..3388
                     /locus_tag="PKK76_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNL68353.1"
                     /translation="MKRRILIVDDHPVVLAGIQRALMDRKMEVHCTRSLKDARSILGS
                     AETFDLVLLDIHLEDGLAFDLALELRKSARDLLVAFLTSSRDWNHLVKARQTGARGFL
                     LKDAELDSIAESIERILSGETVFPGIESPQSISAETAAAVHSLTEREREVMRYTSQGL
                     LNREIADRMNVSLRTVESHRSKAAEKLGARGTIQLASILAELKHLL"
     gene            3498..4169
                     /locus_tag="PKK76_07505"
     CDS             3498..4169
                     /locus_tag="PKK76_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68354.1"
                     /translation="MKLIHTIAILMSLLLTACNSDNGSSNDSSLLLLGLAAATRTASS
                     TTAQTCAQSAANPVSSVSEIGGVCATPTLVQVGDGTVRDTANNILWIQCAMDDDGSPG
                     AVHETSPMACQTIGASQVFTASIGQGLCASLTYAGRTGWKLPDMRQLGAHFKNQAAAP
                     HVVDSALTVLTLSDVTAYNYVTTTTGTGSQVGMVEFKNNSGSGGGMKFAASGTTGIVT
                     CMIKL"
     gene            complement(4159..4320)
                     /locus_tag="PKK76_07510"
     CDS             complement(4159..4320)
                     /locus_tag="PKK76_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68355.1"
                     /translation="MLGRLKNPLGDLSALPGARSMFFLRLNLAKAWTKIILDQGISVR
                     TPKILRVTA"
     gene            4356..4964
                     /locus_tag="PKK76_07515"
     CDS             4356..4964
                     /locus_tag="PKK76_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HNL68356.1"
                     /translation="MVLEKGLRERLKRLTRPFHREVETAFYPARLLSGALTAPQYRDY
                     LQTLHSVHVAWENSWASFSEWSLLGIPRIEQRFRASHAREDLSILGASPLVTSVAWSP
                     STFAHAIGSAYVLEGSTLGGAILGKKIPQIFEGKPEAACVNYFAGRGESAVPLWAEFC
                     GLLDRVDQERPDLVPSIISGACMAFLSMEKELDVDKTGSISM"
     gene            4933..>6933
                     /locus_tag="PKK76_07520"
     CDS             4933..>6933
                     /locus_tag="PKK76_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNL68357.1"
                     /translation="MLTKLDLSQCEKEAIHIPNLVQPHGFALVIEKSSLNVTRSSANF
                     DSTSIGPASQLVGRSLQSILPPAALAAISQKLDFSQRKRHVLYGLAIPSISVSSLNVA
                     VCDAHREIILEFLPGEADGGEEFVELQLNDMIPRVMMAQSSEDFFQRAAIELRSLTGY
                     DRVMVYRFDEDFNGQVIAESHEQGMESYLGLHYPASDIPVQARELYRKNMIRTIVDVS
                     YEPVGIVSRDPAPLDMSYSYLRSVSPIHLEYLKNMGVAATLTVSIIVDNALWGLVSCH
                     HRTPYLPHMKRVGLAEVFGNIFGGMIQMREESERDRVSGRMLSRLDTVMDIVMSQDKN
                     QDVMDLIDQRSFLFQSIFPSDGFLVRTDSQIVRRQFDLDDKLLTGLLDRLAPLMRGSI
                     FYTDHLAAVLPDLPAKTLRECAGVMALQLDTRPPSLWLWRRSEKTQTITWGGDPNQKV
                     ILNQQGVISPRKSFEKYNQIVTGQSTRWNSAERDLPRYLTAQIYRLFEFFESSHQLEN
                     QKKQIQFMEEEKAKHYQELIEMLVGVIEQRDAYTAGHTRRVADYCVAIAQKMGLDEAS
                     VSTLREAAILHDIGKVIIPDSILLKPGRLSFKEYELIKAHLSVGYQILSRIDYYKPLA
                     EIIKHHHEKFDGTGYPEGKKGDEIPLLSHIMIVADAFDAMTTN"
BASE COUNT         1571 a         1673 c         1883 g         1806 t
ORIGIN      
        1 tccaagacga tctttcaacc cctcctggtg atcaatgaag actctgagtg gagcatcaaa
       61 ggactgcact ctggtcacta taaagatctt actcttcgca agggagagag tcgcactaag
      121 atccgcgctg cgggctttga agctcagagt ggatcggttt atgaaatgat gataccgctt
      181 tccgacgttc agcctcgaac taattttcgc cgcgtgcgca ttcactttta tttgttcgcg
      241 ggacagaaac atgattctgt tgtgctggat ttgcgaaagg atgaatctgg aagatgggca
      301 gccgctactt cgcggatgat gccggatgcg gaaagccggg agaggccttc tgttcccact
      361 tgcatgagtg cgtcagagga ttcatgtata gaatcattgc acaggcaatg agtttttttg
      421 attgcataac tttctggttg ggtatcctca gtgccggtat ttcctctggg cgtgcgcaat
      481 cacacacttt ggttcggcag gaccaggcga gccttcgcgt ggttatgcgt tctggcactc
      541 gtttcatgct ttgccggctg ctcttcgcct tccggtcccg ctgcggcggc gggcaggatc
      601 gacctatcat ccactgattt gacgcgggcc actcctctgg acggggagtg gagattcatc
      661 tggcagaagt tcgtggatcc ggcctcgcag gcagaagaag gctggagttt tcaaaaagtt
      721 cccggcttct ggaattctgt tccagggctc caggcggagg gttttgctac ctatcggttg
      781 gacgtccagt tgcctccggg acagcgcctc tatggattgc gcattgtcga catggaatct
      841 tcatacaggc tatttgccgg cactcgcgag atagcggcta atggagtgcc cggcatttct
      901 cgggagacag aaattccaga atggaaacca gcggttggac tatttgaggc gaatggaaac
      961 gttcgtttga ctctctacgt ctccaatttt caccacaggc tgggagggat ctggcaagga
     1021 atcaagatag ggaacgctga aacaatcctc agtgcgcaga gaaaatctct ggcattggat
     1081 ctggctgtaa ctggctcact catcatcatc gcagtgtatc acgctttgat tttttccgtg
     1141 cgtcgaaccg agcgcggctt cattctactg ccgatttttt gtctgatcat ggcgcttcgc
     1201 actctcgtca tcagtgaacg tttcattgca gttatgtttc caggtattcc ctgggatctg
     1261 ctggttcgcc tggattatct aagcgtttcc cttggactca tttcagcgca tgcttatttg
     1321 aaggtagtct ttgcgcgcta ctatcatcgt tttgtttttg tattacttct tgctgttttt
     1381 ggaccctgtg ctgccgccga ggcgtttctc ccggtgcgat ggtttactca tctcttacct
     1441 gttcacaatt tattcctgct attgttcagt ctttattttc tggtcatcgc ctttcaggct
     1501 gtgagggagc gaatgcccgg ggcggctgct atctggttcg gatggttggt cgcaatttcg
     1561 gctaacgtga acgattctct ctatatcatg agcgcaattg attctgctta tctttctgcc
     1621 attggcctac aagcgttcat acttactcag tcctactatc tggcacgacg atattctgaa
     1681 gccttcgcgc tcagtgcgga gttggccagg cgtatgcaaa gtcttctggg cttgacccgg
     1741 gaattcaacc gcgcggcgga tcgtggaaca gctgttcgtc gggcgttgcg agagatcagc
     1801 gacattctgg aaaagccaga ccttcaggcg ttcattcctg tgagagattc acctctgatg
     1861 cgaaagatct cagcggacag accggatgaa ttcgctgatc tcccctggtc cacaaaggcg
     1921 gaatccatca catcacaggt tcaggaagac ggtcgcgtgc tcattccctt gcgtggcgga
     1981 ggagagctac tgtgcttgat tgatttgccg gggtttgagg acgctagaac gcaatacgtg
     2041 caggggattc ttgattctct gtctctcgtt ctagataaca tgcgaagagt ggaaagcgta
     2101 gcgctcgcct cgattggaca ggcggccgct gaaatcgttc atgacatcaa ccatcactgt
     2161 caggtgatca aggcgcacgc ttcctctggc cagcattcag tggaaaggct tcgtttgatt
     2221 gaaaatgaaa ccgggcttat gacgaacctc gccctggaca ttctagactt cgccagagag
     2281 aatgtgatcg taaatcctat ggccgtgtca ctggaaagtg taaaggatgc gttcaatcag
     2341 gacattggat ctgccttcat gaataccgat atgcggcatt cgattgtagt gaacgctgca
     2401 ggagacattc gcattgacct ggagcgattc cgaagagtgt gtctgaatct tgcacgaaat
     2461 gcaatggagg catgcaatgg atccggattg tttcgcgttg aaatcgaaat cggctcggat
     2521 acagtgatca tggttttcga agacgatggc ccaggaattg atcctgccat gcaagagaag
     2581 ttgtttgaac cgtttgcaag tggcggccgc gggacagggc ttggcctggc agtggtcagg
     2641 aggattgttt ccgcgcacgg tggtgacatt cgagtttatt cgactcccgg aaaaggatgt
     2701 cgttttgtag tgatggttcc tttgaatttc ggttgaccag gtacagaaag cggggagcca
     2761 gaaagagttg aaaagaagaa ttcttattgt agatgatcat ccagttgtac tcgccggtat
     2821 ccagcgcgca ttgatggatc ggaaaatgga agtgcactgc acgcggtctt tgaaagatgc
     2881 ccgatcaatc ctgggaagcg cagaaacatt tgatctagtg ttgctcgaca ttcatctaga
     2941 agatggtctt gcgtttgatc tggcattgga gctgagaaaa tcagctcgcg acttgctggt
     3001 tgcgtttttg acttcttcgc gcgactggaa tcatctcgtg aaagccaggc agactggcgc
     3061 gcgcggtttt cttctcaaag atgcggaact tgattcgatt gcagaaagca ttgaacgcat
     3121 cctctccggg gagacggtct ttccaggaat agagtctcct cagagcatat ctgcggaaac
     3181 tgcggcagca gttcacagtc tgacggaaag agaacgagaa gtaatgcgat atacatcgca
     3241 gggactcttg aatcgtgaaa ttgcagatcg catgaacgta agtctgcgca ccgttgaatc
     3301 gcatcggtca aaagcagcag aaaagctcgg cgccaggggc accatccagc tggcttccat
     3361 tctggccgag ctcaagcacc ttctttgaaa tttctttaaa gattcctggt agaacccgca
     3421 ttttttagat catctttgcg gttccttctg ctgacaaagg cgtgtttccg tgcgatcctt
     3481 tgcacggaga tacgaacatg aaactgattc acaccattgc gatcttgatg tcccttttgc
     3541 tcactgcttg caactcagac aatggatcga gcaacgattc gtctctgttg cttctcggcc
     3601 ttgcggccgc aaccaggact gcgtcgagca ccaccgctca gacttgcgca caaagcgcgg
     3661 ctaaccctgt gtccagtgtt tctgaaatcg gcggagtctg tgcgacgccc acgctcgtgc
     3721 aggtggggga tggcacggtg cgcgatactg cgaacaacat tctctggatt cagtgcgcga
     3781 tggatgacga cggatctccg ggagccgtac atgaaacaag tccgatggct tgccagacca
     3841 ttggcgcttc gcaggtgttt acagcgtcca taggtcaagg cctctgtgcg agtctcacgt
     3901 atgcgggccg caccggctgg aagttgcctg acatgcgtca actcggtgcc cattttaaga
     3961 atcaagcggc tgcgcctcac gtagtggatt cggcgctaac agtgctcact ctgagtgacg
     4021 tgactgctta caactatgtc acaaccacaa ctggaacggg tagtcaggtt ggcatggttg
     4081 agtttaagaa caactctggt tctggcggtg gaatgaagtt cgcggcatcc ggaaccacgg
     4141 gtattgtaac ctgtatgatc aagctgtaac gcgaaggatt tttggcgttc gcaccgaaat
     4201 tccctggtcg aggatgattt ttgtccaggc ttttgctaga ttgagtctca gaaagaacat
     4261 ggaacgtgcg ccggggaggg cgctgagatc gcctaacggg tttttgagtc gaccgagcat
     4321 cctgttttcg attactggaa cgcggcggtc gaatcatggt attggagaag ggacttcgcg
     4381 aacgtctaaa gagactcact cgcccgtttc atcgcgaagt agaaactgct ttctatccag
     4441 caagacttct ttctggagca ttgacggcgc cacagtaccg tgactatttg cagacgcttc
     4501 attccgtcca tgttgcgtgg gagaattcct gggcctcttt ttcagaatgg tcactactcg
     4561 gcatcccgcg tatagaacag cggtttcgcg ccagtcatgc cagagaggac ttgtcgattc
     4621 taggtgcaag ccctctggtc acttccgttg cctggagtcc gtctacattt gcacatgcga
     4681 tcggttccgc ctacgtgctt gaaggttcca ccctgggtgg tgcgattcta ggaaaaaaaa
     4741 tccctcagat cttcgagggc aagcctgaag cagcctgtgt gaattacttt gcggggcgag
     4801 gagaatcggc tgtaccctta tgggcggagt tctgcggatt gctggatcgc gtggatcaag
     4861 agagaccgga tcttgtccca tccataatat caggtgcgtg tatggcattt ctttcaatgg
     4921 agaaagagct cgatgttgac aaaactggat ctatctcaat gtgaaaaaga agccattcac
     4981 atcccgaatc tcgtgcagcc acacgggttc gcgctggtca tcgaaaagtc cagcctgaac
     5041 gtcacgcgtt cgagcgcgaa tttcgattct acatccattg gtcctgcgtc ccagcttgtg
     5101 ggcagaagct tgcagagtat tttgccgcca gcagcgctgg ctgcgatttc gcaaaaacta
     5161 gatttttcgc agagaaaaag gcacgttctc tatggccttg cgattccttc catttctgtc
     5221 tccagtctga acgtcgcggt ctgcgacgcg catcgcgaaa tcattctgga attcttgcca
     5281 ggagaagcag acggagggga agagttcgta gagcttcagc tcaatgatat gattccgcgc
     5341 gtgatgatgg cgcagagttc ggaggatttt tttcagagag cggccattga attgcgttca
     5401 ctgaccggtt atgatcgagt catggtctac aggttcgatg aagatttcaa tggtcaggtg
     5461 atcgcagaat cgcacgagca gggaatggaa tcttatttgg gcttgcacta tcccgctagc
     5521 gacattcctg tgcaggcgcg tgagctgtat cgaaagaaca tgattcgcac gatcgtagat
     5581 gtgagctatg agccagttgg gattgtttcg cgcgatcctg cgccgctcga catgagttat
     5641 agttacctgc gaagcgtttc ccccattcat ctggagtacc tgaagaacat gggtgtggcc
     5701 gccacactga cagtctccat cattgtcgac aatgcgctct ggggacttgt cagttgtcat
     5761 catcgcacac cgtatttgcc tcacatgaag cgcgttggcc ttgcggaagt tttcggcaat
     5821 atatttggcg ggatgattca gatgcgtgaa gagagtgaac gcgatcgtgt gagcggtcgt
     5881 atgctttcgc gtctggatac tgtcatggac attgtcatga gccaggataa gaaccaggac
     5941 gtgatggatc ttattgatca aagaagcttt ctgtttcagt ccatttttcc atcggatgga
     6001 tttctggtgc ggaccgattc gcagatcgtc agacgccaat tcgatctaga tgataagttg
     6061 ctcaccggac tactcgatag acttgccccg ctcatgcggg gatccatctt ctatacggat
     6121 catctggcgg cggtattgcc tgatcttcca gcaaaaactc tacgagagtg cgcgggggtt
     6181 atggctcttc aactcgacac caggccacca agtctgtggc tctggcggag gagcgaaaag
     6241 acccagacca tcacatgggg cggggatcca aatcaaaaag tgattttaaa tcagcagggt
     6301 gtcatctcgc cgcgaaaatc attcgagaaa tacaatcaga tcgtgaccgg ccaatccacg
     6361 cgctggaatt cggccgaacg cgatcttcct cggtacctta ctgcacagat ctatcgattg
     6421 tttgagtttt tcgaatcaag ccatcagctg gagaatcaaa agaagcagat ccagttcatg
     6481 gaggaagaga aggccaagca ttatcaggaa ttgatcgaga tgctcgtcgg tgtcattgag
     6541 cagcgcgacg cgtacacggc cggtcatact cgtcgcgtcg cagattattg cgttgcgatt
     6601 gcgcaaaaga tgggccttga tgaggccagt gtttctactc tgcgcgaagc ggctattctt
     6661 catgacatag gaaaggtgat catccctgat tctattctcc tcaagcccgg gcgactctcc
     6721 ttcaaggaat atgaactcat caaagcacac ttgagcgtcg ggtatcagat tttgtcgcgc
     6781 attgattatt acaaaccgct ggcggaaatc atcaaacacc atcacgagaa attcgacggc
     6841 actggttatc ccgaaggtaa gaaaggagac gaaattcccc ttctttcgca catcatgatc
     6901 gttgcggacg catttgatgc gatgaccacc aac
//
LOCUS       DAORFF010000144         6929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5586_length_6929_cov_3.816992, whole genome shotgun sequence.
ACCESSION   DAORFF010000144 DAORFF010000000
VERSION     DAORFF010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5586_length_6929_cov_3.816992"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PKK76_07525"
     CDS             complement(<1..410)
                     /locus_tag="PKK76_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL68358.1"
                     /translation="MQSSAPSDTFSPYQKFIIVTIAVLQFTVILDFMVLSPLSALLLK
                     ELQVTTTQFGLVVSAYAFSAFASGIAAAGFADKFDRKRLLLFFYIGFLIGTLMCGLAP
                     DYYSLLVARIVTGLFGGVLGSIGGAIVTDLFPMSA"
     gene            complement(492..1628)
                     /locus_tag="PKK76_07530"
     CDS             complement(492..1628)
                     /locus_tag="PKK76_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68359.1"
                     /translation="MRRFDPKYFLLFALATCSPQAQPGSSGDAPATSHYNDVARYLAG
                     LPIENPSAEVRKAIETPLYKNHVALMNQSWSAIEQTSLIPMREWKKQNLTDACEERLA
                     FYPLSGADFGNFYTMLPDCKNYLMIGLEDAGSVPEPFQLNDAQLASGLGQLERMVGQI
                     SIRNYFWTESMKTETANAYLEGVTPAILVFASRLGLNIRNIESVQVNDEGKVVVTRAV
                     PKDQRLKTLSGIRITFRAPGDKEDRTLQFLRIRIGPAAGSDTAPEGKFFKQYGDFITM
                     FKSAEYLMHFKLGEEFRSFVLQRSRLVIQDDSGIPYDKFTPEWTIKCFGDYVHPYPLV
                     QYTPQVQPGLAAAFAKERYPLPFPFGYGFYQGKGKSNLVIARKK"
     gene            complement(1728..2810)
                     /locus_tag="PKK76_07535"
     CDS             complement(1728..2810)
                     /locus_tag="PKK76_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68360.1"
                     /translation="MTRIAVISTLFLTLFAIPVFAEDHRFLVRGDAALGSISNAEAAE
                     DDYFYPLNNAIAGSLTIPSTTPSYKCPASLKPRMFETPEGSQERKDGCRSMASGVRAE
                     YRFQNMVRVFYDRFSLPNASYSTNFNTPVFAPGVNGVSQLQFGYNKREGRTRGGVGFY
                     YPVLEKLHIGVHIAQEKNFMHFGVTPASYTLYRVGNSSLSGLVAGAGIRELQTRGATP
                     GAGIEFDPMEYFGISLEYDRIRQRGSYSDNRVSGAASSTAGILSIGNPVIETIQAGLT
                     HSGYEAGLDLRFGKGRPIAFYLGGSQRKYVRSYQSYFYTTNSTFLAGPTITVLNNLYL
                     AGLYTSHRFIQNENLFHFKMELGLGF"
     gene            complement(3132..3890)
                     /locus_tag="PKK76_07540"
     CDS             complement(3132..3890)
                     /locus_tag="PKK76_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68361.1"
                     /translation="MVRYGQPRSAELVLITVAEPMDAKQRVKSDKPGPDIVYALKQNQ
                     ALNFQTGVYPYHYLNSLFWQIDNGRLLKAAMTSQEWCGTTTKVLLPQGGLDRLMYHSY
                     WDNEADGEMVLVRPDVQHPDRAILYDELPLIVRTPEAEHYRKLQVFPLLMSSQVTKPD
                     WDIYGEKRSPAYEPGTLEPESVDFMAMGVLRKARKITVRSGPGLKLVDEYFVEQNPAR
                     TLLGWNRHDGAHFVLRKISYAEYWKLNKPGDTLP"
     gene            3955..4986
                     /locus_tag="PKK76_07545"
     CDS             3955..4986
                     /locus_tag="PKK76_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNL68362.1"
                     /translation="MKRGGLAGGPAQADMKNIPAQTEKILACSTLRIAYGTLSFFQGK
                     KKMNLRRDHIQPIQIAVQLASTPQAAHRAITSQTELRKWWAPRVIMSRNIVSQLNGKD
                     MEMKILQEDKYRLIRFTWRGADWKRDVPETVITYEITDLGAARGSTGQGIQIEMNHDG
                     WSDEEERNLQENIWQQALPGLQALLEGKKFTPWWEDEKNRSGDTGYKTADLKAFLERS
                     DKEGRGKNDRKKSIQVIWKICQAMDGVGEWIVKESTGEFELRARGALLFASLKGGILS
                     LNWRELEKLLGRELVDFANRLSLEQDLDLQIGKTQEKIIGKDLNPDLFSQWLQDVLDH
                     QKSDSKKKK"
     gene            5005..5640
                     /locus_tag="PKK76_07550"
     CDS             5005..5640
                     /locus_tag="PKK76_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNL68363.1"
                     /translation="MLPHVYSQATGMISFSWTALSEHIFEIRIKTPVTRVGTLYRLTA
                     AIYVLGLDIVSGTVDTAHEHGEEYADDTFLLRPSSDGETSFAYYSARVGVLMETLIRN
                     EKDPDDLLRERNINPPDIRFLFEALPEITFRDIPDRKVTEFNITSQNRTGLLFHITRI
                     FAEEGISILRGTIKSEEDTAKDRFFLQQQGECISEESKSRLSAKITGQPAS"
     gene            complement(5658..6485)
                     /locus_tag="PKK76_07555"
     CDS             complement(5658..6485)
                     /locus_tag="PKK76_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68364.1"
                     /translation="MPSDKYIPIESVEDIDVLKLSPRDLDKRYIDRNGNRYALRFNMK
                     TRRPEVIQIVSNRLEARRLQQTILLEKRKPEKSQATTAESVSLPPPVFVPDEEAFHES
                     RFVDECVSELPKLKESQQASVAKIRQSRVFETVQSAEFLDLMRDIERDAWRKTDENVQ
                     QCREWISRPPSLHAALIRVQPDKRKILDSIPDDPVKLEAMRRWEWQDRLTDSFTRLND
                     FAVRLKQMLASVSDEELLKVPQNQRQFFKDSGASLDIIQDACEEKLARLDVWRKRFL"
     gene            complement(6532..>6929)
                     /locus_tag="PKK76_07560"
     CDS             complement(6532..>6929)
                     /locus_tag="PKK76_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004468186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNL68365.1"
                     /translation="RIVFNNDDIIGQKVELFSQHLDTLYPMLYPSHFYGELDRRKYPY
                     KTIYEGVKASRARSGNTRIVAYIQGFPMRVHESGLSLNDYIKDQIKASEDCGGDGFIV
                     WHAANQYGPFFAAYAAYQKEKAARLASGK"
BASE COUNT         1855 a         1717 c         1709 g         1648 t
ORIGIN      
        1 gcagacatgg gaaacaaatc cgttacgatg gctccaccga tagaaccaag aactccaccg
       61 aatagtcccg taacgattcg tgcgaccagg agtgaatagt aatccggtgc cagtccacac
      121 atcagcgtgc cgatcaaaaa cccaatatag aagaacagta acaatctctt acgatcaaat
      181 ttgtctgcga atcccgccgc ggcgatccca gacgcgaacg cgctgaacgc gtaggcggaa
      241 acgacgagcc cgaattgagt ggttgtgacc tgtaactctt tcagcaggag cgcgctgaga
      301 ggtgagagaa ccatgaaatc aagaattact gtgaactgta gaactgcgat ggtgacgatg
      361 atgaatttct ggtaaggcga aaatgtgtcg gaaggagccg aactttgcat gagatctcct
      421 gggatttgtt acaagaacat aaagaaggga gagaacgcca tgcatttgcc caggcgatgg
      481 cgcgtttttt tctacttctt gcgagcgatc actagattgg acttaccttt tccctggtaa
      541 aacccgtatc cgaacggaaa gggaagagga tagcgttcct tcgcgaatgc agcggccaga
      601 cccggctgca cctgaggagt gtactgcacc agcggatatg gatgaacata gtcgccaaag
      661 catttgatgg tccactctgg agtgaatttg tcataaggaa tgccagaatc atcttgaatc
      721 accagacgac tgcgctgcag tacaaagcta cgaaactctt cgccgagttt aaagtgcatc
      781 aagtattctg cggatttgaa catggtgata aagtcgccgt actgtttgaa gaattttcct
      841 tcgggcgctg tatcgctacc tgccgcaggt ccaatccgaa tccgcaggaa ttgaagagta
      901 cgatcttctt tgtctccagg tgcgcggaac gttatgcgaa ttccgctcaa agttttgagt
      961 ctctgatcct ttggcacagc tcgggttaca acgacttttc cttcgtcatt tacctgaacg
     1021 gattcaatgt tgcgaatgtt caaaccaagt ctggatgcaa aaacgagaat cgcaggagtc
     1081 actccttcga ggtaggcgtt cgctgtttca gttttcatgg actctgtcca gaaataattg
     1141 cgaatggaaa tttgtcccac cattcgttcg agctgaccca ggccgctggc aagctgcgca
     1201 tcattcaact gaaatggctc tggaacagag cccgcgtctt ccagaccaat cattaaatag
     1261 ttcttacaat ccggcagcat ggtatagaaa ttcccaaaat cggcaccgga gagaggatag
     1321 aatgccagcc tttcttcgca cgcgtccgtc aggttctgtt tcttccattc gcgcatggga
     1381 atcagactgg tttgctcaat ggcgctccag gattgattca tcagagccac gtggttctta
     1441 tatagaggcg tctcgattgc tttgcgaact tccgcagaag gattctctat aggaagaccc
     1501 gccaggtatc tggctacatc gttgtagtgc gacgtggctg gagcatctcc ggatgaaccg
     1561 ggctgcgcct gcggagaaca ggttgccagc gcgaataata aaaagtattt aggatcgaat
     1621 cttctcatgg ccttatgcca ggaaacagcc gaaagtgctg taggcaagcg aaaacaagac
     1681 cagggaagcg agtgcttcgc ggaggccatc cggactccgc gaacgaatca aaagcccaga
     1741 cccagctcca tcttgaaatg aaacaggttt tcattttgaa taaaacgatg agaggtatac
     1801 aggcctgcga gatacaggtt gttcaaaacc gtaatggttg gaccggcaag aaacgtactg
     1861 ttcgttgtat aaaaataaga ctgatagctc cttacgtact ttcgctgcga acctcccaga
     1921 tagaacgcaa ttggccgacc cttgccgaat cgcagatcca gacctgcttc ataaccggag
     1981 tgcgtgagac cggcctggat tgtttcaatc acagggttgc ctatggagag aatgccagct
     2041 gtagaactcg cggcgccgct gacgcgattg tcgctatagc taccgcgttg gcggatgcga
     2101 tcatactcaa gagatatacc aaagtattcc attggatcaa actcaatgcc cgcacccggc
     2161 gttgctccac gcgtctgcag ttcgcgaatc cccgctccag ccacaagtcc gctgagagaa
     2221 gaattcccta ctctgtacag agtgtaggat gcaggggtta ccccaaagtg cataaaattc
     2281 ttttcctgag ctatgtgcac gccaatgtga agtttctcaa ggaccgggta gtagaaacca
     2341 acacctcctc gcgtacgtcc ttcccttttg ttatagccaa attgcagctg cgaaacgcca
     2401 ttcacacccg gagcaaaaac cggagtattg aagttggtgc tgtagcttgc gttgggtaag
     2461 ctgaagcgat cataaaagac gcgaaccatg ttctggaaac ggtactcggc tcgcactcca
     2521 ctggccatac ttctgcaccc gtccttacgc tcctgagaac cctcgggtgt ttcaaacatc
     2581 ctgggtttca aacttgcggg acatttgtag gagggcgtcg tagaaggtat ggtcaaacta
     2641 cctgcgattg cgttgttgag cggatagaag taatcatctt cggccgcttc tgcattggaa
     2701 atagatccaa gcgccgcatc tccacggacc aggaatcggt gatcttccgc aaataccgga
     2761 atagcgaata acgttaagaa caaagtagat atgacagcaa ttctagtcac aaaaatcctc
     2821 caaaaatagc aaaaccaact cagtaggttg aagtgcaatt tgagttcttg tgttttacta
     2881 tctgtgcacg acagagaaag cgtacatgag cagactacca gagccattgc agtcttgaaa
     2941 attgaaatcg atcgtgaact catttcacaa ccgcaatctc agcgtgcgca tttgcgatca
     3001 agcaatacgc aaagttttat aaactatttt cagtttttgt aaactgatat gctgttcatt
     3061 accagattca agacgttcat tcaatgattc ggaatttgtg aacgttcagt ctcagcgcca
     3121 ggtgaaaaac atcagggcag agtatcgcct ggtttattca gtttccagta ctcagcatag
     3181 gagatcttac gtagtacaaa gtgagctcca tcgtgacgat tccatccaag gagagtcctt
     3241 gcaggattct gctccacaaa atactcatct acaagtttaa gtccagggcc gctgcgaacg
     3301 gtgatcttgc gagccttgcg caaaactccc atcgccataa aatcaacaga ctcgggctca
     3361 agtgttcccg gttcatatgc cggacttctt ttttctccgt agatatccca gtctggcttt
     3421 gtgacttgcg acgacatcaa aagaggaaat acctgcagct tgcgatagtg ttctgcttcc
     3481 ggcgtccgca caatcagagg caactcatcg tacagaatag cgcgatcggg gtgttgcacg
     3541 tccggtcgaa cgagaaccat ttctccatct gcttcattgt cccagtaaga atgatacatg
     3601 agtcgatcca gcccgccctg aggaaggaga accttggtgg tagtgccgca ccattcctgc
     3661 gaagtcatgg cagctttcag aaggcgcccg ttgtcgatct gccagaagag ggaattcaga
     3721 tagtgatatg gataaacgcc ggtctgaaaa ttgagcgcct gattctgctt gagcgcgtac
     3781 acaatgtccg ggcccggttt gtctgatttg accctctgtt ttgcgtccat aggctctgcc
     3841 accgtgatca aaaccagttc tgcgcttctg ggctgcccgt atcggaccac agaggcatca
     3901 taaatggcga tctccgccct gccctgaaac cctggttcct ttaaaaaagg gaaagtgaag
     3961 cgaggcggac tggccggcgg gccggcacag gcggatatga aaaacatccc cgcgcaaaca
     4021 gaaaaaatcc tagcatgcag tacgcttcgc atagcgtatg gaactctctc tttctttcag
     4081 gggaagaaga aaatgaactt gcgcagagat catatccagc ccattcaaat tgccgttcag
     4141 ctggcctcca cgccgcaggc cgctcatcgc gcgatcacct cccagacgga attgcgaaaa
     4201 tggtgggcgc cccgcgtcat catgtcacgc aacattgtct cacaattgaa tggaaaagac
     4261 atggagatga aaattctcca ggaagataaa tacagactca tccgatttac gtggagaggc
     4321 gcagactgga agcgcgatgt tcccgaaaca gtcatcacct acgaaatcac agatctgggc
     4381 gcggcacgcg gaagcacagg ccagggcatc cagatcgaga tgaatcacga cggctggtca
     4441 gacgaagagg agcgaaatct ccaggaaaac atctggcagc aggcccttcc aggactgcag
     4501 gcactcctgg aaggaaagaa attcactccc tggtgggagg atgagaagaa tcgttcaggg
     4561 gataccggtt acaaaaccgc ggatctcaaa gcattcctcg aaagatcaga caaagaagga
     4621 cgcggcaaga acgatcgcaa aaaatccatt caggtaatct ggaagatctg tcaggcaatg
     4681 gacggagtcg gcgaatggat cgtcaaagaa agcacaggcg aatttgaact tcgcgcaaga
     4741 ggtgctcttc tattcgcatc tttgaaaggt ggaatccttt ctctgaactg gagagaactg
     4801 gaaaaactct tgggcagaga gcttgttgat tttgcgaatc gtctatctct tgaacaggat
     4861 ctcgatctac aaattggcaa gacgcaagaa aagatcattg gcaaagatct gaatccagac
     4921 ctgttctcgc aatggctaca ggacgtgctg gatcatcaaa agagcgactc taaaaagaag
     4981 aaatagcttt ttggattgac gggcgtgctt ccgcacgtat attcacaggc aaccggaatg
     5041 atctcttttt cctggactgc cctgtccgaa cacatttttg aaattcgaat caaaacaccg
     5101 gtaacgcgag tgggtaccct ctatcgcctg acagctgcta tctacgtcct gggtctggac
     5161 attgtatcgg gaacagtgga tacagctcac gaacacggag aagaatacgc agatgatact
     5221 tttctactac gcccttcttc agacggagag acaagtttcg cgtactattc ggcacgagtg
     5281 ggagttctca tggagactct cattcgaaat gaaaaagacc cggacgattt actgcgcgaa
     5341 cgcaatatca atccaccgga cattcgcttt ctcttcgaag ctcttccaga aatcaccttc
     5401 cgcgatattc cggatagaaa agtaaccgag ttcaacatca ccagccagaa tcgcactggt
     5461 ctgctatttc acatcacacg cattttcgca gaagaaggaa tcagtatcct tcgcggaacg
     5521 atcaagagcg aagaggatac tgcgaaggat agatttttcc tgcagcaaca gggagaatgc
     5581 atcagcgaag aatcgaaatc caggctcagc gcaaaaatca caggccagcc agccagctaa
     5641 ccaggtctgc gcgaaaatca aagaaagcgc ttacgccaga catccagtcg agccagcttc
     5701 tcttcacatg catcctgaat gatatccaga ctcgcaccgg aatctttaaa gaactgacgc
     5761 tggttctggg gaactttaag cagctcctca tctgaaacag aagcgagcat ctgcttgagt
     5821 cttactgcaa agtcattgag tctcgtaaaa ctgtctgtaa ggcgatcctg ccattcccat
     5881 cttcgcatgg cttcgagctt cactggatcg tctggaatcg aatctagaat ctttctcttg
     5941 tcaggctgta cgcgaatgag tgcggcgtgc aaagaaggcg gacgcgaaat ccattctcta
     6001 cattgctgca cgttctcatc tgtcttgcgc catgcgtccc tttcaatatc gcgcatgagg
     6061 tccaggaatt ctgcggattg tacagtttca aatacccgcg actggcgaat cttcgcaacc
     6121 gatgcctgct ggctttcctt taactttggc aattccgaga cacactcgtc cacaaatctg
     6181 ctttcgtgaa atgcttcttc gtccgggaca aacacgggcg gcggaagact cacagattca
     6241 gctgtggtgg cctgcgactt ttccggtttg cgcttttcta acaaaatggt ttgttgcaac
     6301 cttcttgcct caagcctgtt ggatacaatt tgaattactt cggggcgcct tgtcttcatg
     6361 ttgaagcgaa gcgcataacg gttgccgttg cgatcgatgt accgcttatc gaggtcgcga
     6421 ggcgaaagct tcaatacatc aatgtcttct acagactcaa tcggtatgta tttgtccgac
     6481 ggcatggcgt gactcaaaca tgactgatca gaatttcaag cagtattgtg cctatttacc
     6541 cgatgcgagc ctggccgctt tttccttctg ataggccgca taggcggcga agaaaggacc
     6601 gtactggttg gccgcatgcc atacaatgaa gccatctccg ccgcaatcct cggaagcctt
     6661 gatctggtct ttgatatagt cattgaggct gagaccggat tcgtgcactc gcataggaaa
     6721 gccctggatg tatgcgacga tcctggtgtt gcccgagcga gcgcgggatg cttttacgcc
     6781 ttcgtagatg gtcttgtaag gatacttacg tctgtcgagc tctccgtaga aatgagaagg
     6841 atagagcatt ggatacaatg tgtccaggtg ctgggagaac agttccactt tctggccaat
     6901 gatatcgtcg ttgttgaaca cgattcttc
//
LOCUS       DAORFF010000145         6900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5625_length_6900_cov_4.209642, whole genome shotgun sequence.
ACCESSION   DAORFF010000145 DAORFF010000000
VERSION     DAORFF010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5625_length_6900_cov_4.209642"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1429
                     /locus_tag="PKK76_07565"
     CDS             <1..1429
                     /locus_tag="PKK76_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNL68366.1"
                     /translation="VLKNRGSLQDIDQIAAVKPHVKWHASAKKVRDLPRLIATAFMKS
                     REGVPGPVFVEVPVDLLYEESTVREWYMASVPRKPGLKNYVISRYLSRHVDTLFRGAD
                     LSSPASPMPYNVPSHTDSDLRQVVERLQTAAKPLLVIGSQAMLQPQRSAELARAVDQL
                     GIPVYLAGMARGLLGKKHKLQLRHKRAEALKECDLVILAGVPVDFRLNYGSQIRSSAF
                     HIGVNRSSQDLKKNKRPSLALKADVCDFLISLSSVYSSGAGRLDAWISQLRARDLKRE
                     EEILSLSAKKAKNVNALKLLFQLKNKLPDNAILVADGGDFVATAAYILEPPAPLSWLD
                     PGVFGTLGVGAGFALGASVVCPDREVWIIYGDGSAGYSIMEYDTFVRHRFPVIGLIGN
                     DACWTQIYRDQKEILKDDIACMLLHTDYHKMAEATGATGFLIARESSVASGLTAARKA
                     AQKKSPVLINAIIDISDFRKGSISM"
     gene            1445..3103
                     /locus_tag="PKK76_07570"
     CDS             1445..3103
                     /locus_tag="PKK76_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNL68367.1"
                     /translation="MHFPWTKHYDAHVPPVLEYPDSTIGELFDESVRKHGSRIAVQFM
                     GKKISYDSMEKDAASLAVHLSSKGVRPGDRVFLLLPNTPHFVAAYYAVMKIGAVVVPA
                     SPLDTAPEIQYKIRNSGARVAVFLDLLFESLEPSLPLLEAAVGCDLSDYLPFPKNYLF
                     PIKKRRLGRKMPPYNEKVIRFSEILKVKETFPCKHSSPDDLAVIIYTGGTTGISKGVM
                     LSHRALAVNMTQARTWGQVTGDDINLCALPFFHGFGMSIGLNTTFVAGGRMLLLPKWD
                     AGQAIRHFEKDGVTLFAAVPTMYNSILNHPDFSRMKNARLRGCYVGAAPVPESLKEEF
                     FRRTGGILIEGYGLTEAVTAKSANPYIGKKKEKSIGIPWPDTEFRIVDEITGEASAPG
                     VEGEIILRSPDLMMGYWQNPEATDEVLRDGWLFTGDIGRMDEDGYFYIVDRKKDLIIS
                     GGFNVYPSEIEEVLYSHPSVLEACVIGVRDTHKGEIPRAYVVLRQGEREDANALMEFL
                     GVRLIHYKVPRSFIFKDALPKSPIGKILRKELRAADPDVGSSRG"
     gene            complement(3111..3905)
                     /locus_tag="PKK76_07575"
     CDS             complement(3111..3905)
                     /locus_tag="PKK76_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNL68368.1"
                     /translation="MDYLIKDLSKMTGIASHAIRKWQERYNILRPFRTPNGYWHYSND
                     DYFVLKSIQMRLDQSQKLKKIMALGREALLKDKVTTFSEEQLTFIKLLQAHDYDSVEK
                     IFDKQLKGSFSAWVHHIIGPAVILVGKAWEENLLSVPEEHAFSHWLHGYIFAKIEKFP
                     PHAKPGWLVTTFPGDPHDLSSLLHYAVLRSRGISAWYTGNLPENDLLREVESGKYIGI
                     SISVVLEQPEKKVKALESAILARNPKIKIRFGGFGMSMRKKNKKAR"
     gene            4008..5066
                     /locus_tag="PKK76_07580"
     CDS             4008..5066
                     /locus_tag="PKK76_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009882 - blue light photoreceptor
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HNL68369.1"
                     /translation="MKRLTYISNFSQPLARNEIERIGEVSVRNNARDGLTGALFCFHD
                     VFYQVIEGEEQAIERCYSRILKDSRHKDIFCLKVEQGVSSRMYGDWAMKTVILEESGE
                     AILVALRNMMDGLSSTHRILERYAPAEILQTMQKGRNPLSENSSMTERVVIFADILAS
                     TTLTEVLPAETMALLLADYYEIANAAIVESGGTISKLTGDGLMAHFETGKADSALDAS
                     IRIIHRINDLRSRAESHDPRKFLYSGIGISAGRVLEGSIGSDLRKDYTLLGDPVNSAA
                     RLESVTRKTGHAIVFDSRFRSLLRKERTLKKLGRYLPKGKTDQLQIFTIPEDAIKFHI
                     TSESLRKSIQSLSEPARL"
     gene            5160..6188
                     /locus_tag="PKK76_07585"
     CDS             5160..6188
                     /locus_tag="PKK76_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNL68370.1"
                     /translation="MRTAAEQQATYRLRLRASVVSIVVGFLVCLLKFAGFLTTGSYAI
                     LSDALESIINVAAASFAAYCIVQGARKADFDHPYGRGRLEYFSASLEGALILLAGVII
                     LNESIPKLFAGNVVGRMDLGLMFTGVGTVANGILAAFLIRSGKKHHSMALEADGHHVW
                     SDVVSSIGLFVGLVLVAISKLPWLDPLLACIMALWILWSGFRILRQSFFQLMDRASPD
                     VVTKVTSAIAELRKPEMIHPHRMRLRESGPYVLVDFHMIVPRFFTVRQLHDLEEHIHR
                     NLESILDRPVDLMLHNDPCTSNNCGYCSMKNCRVRKKKQTSQLKLDPQHLLEDISHPV
                     HTRDRRGD"
     gene            complement(6192..>6900)
                     /locus_tag="PKK76_07590"
     CDS             complement(6192..>6900)
                     /locus_tag="PKK76_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68371.1"
                     /translation="RGDCKNGPGTLQLPNGSRYEGEFKNGNFHGQGVYTLPSGVYTGE
                     FKDNKIEGKGVFLLKTGRKLEGNFVNGKLNGEGTETLPDGTRTQGQFESNKFVKGTLT
                     RPDRTTFTGSFTDGQLQGKGESTLPDGSNYTGEFVNGVFQGKGTLTHSDGGVYTGEFV
                     NGKKEGKGIYKYANGDQYTGDFKNNFKEGTGVYSYADKRKYEGQFQNDQPHGHGTYTA
                     QDGKSRTGTWVRGQEQK"
BASE COUNT         1577 a         1658 c         1796 g         1869 t
ORIGIN      
        1 tgtgcttaag aatcgcggtt cattgcaaga tattgaccag attgctgcgg taaagccgca
       61 cgtgaagtgg catgcctcag caaagaaggt ccgcgatctg ccgcgcttga ttgcaactgc
      121 tttcatgaaa tctcgcgaag gagttcccgg accagttttc gtggaagtac ccgtggattt
      181 gctatatgaa gagtcaactg tgcgtgaatg gtatatggcc agcgtgcccc ggaagccagg
      241 gctcaagaat tacgtgatat cgcgatatct atccagacac gtggacactc ttttccgggg
      301 agcggatctt tcttcgccag ccagtccaat gccgtataac gttccctctc atactgattc
      361 tgatttgcgt caggttgtgg agcggctaca gaccgccgca aagccgctcc ttgtgattgg
      421 aagtcaggca atgctgcagc cacaaagatc ggcagaactg gcccgcgctg tggaccaact
      481 tggaattcca gtctatctgg caggaatggc gaggggactt ctgggcaaga aacacaaatt
      541 gcagctgaga cacaaacgcg cggaggcatt gaaagagtgt gatcttgtca ttctcgcagg
      601 tgtgcctgtt gacttcagat tgaattatgg atctcagatt cgatcttccg cctttcatat
      661 tggggtgaat cggagttccc aggatcttaa gaaaaacaaa cggccttccc tggcgctcaa
      721 ggcggacgtg tgcgattttt tgatttcgct ctcttctgtt tattcgtccg gagccggcag
      781 gttagatgca tggatctcac agctacgcgc gagagatctg aaaagagagg aagagattct
      841 ttctctgtcc gcgaagaaag cgaagaacgt gaatgcgctc aagctactct ttcaactaaa
      901 aaacaaactg ccggataatg cgattcttgt cgctgatggt ggagattttg tggccaccgc
      961 cgcctacatt ctcgaaccac cggctccact ctcctggctc gatcccggcg tttttggaac
     1021 tctgggcgta ggcgcagggt ttgcgctcgg cgcaagtgtg gtttgtccgg atcgcgaagt
     1081 ctggatcatc tatggagatg gatccgccgg ctacagcatc atggaatacg atactttcgt
     1141 gcgtcatcgc ttcccggtga ttgggctcat tggcaacgat gcctgctgga ctcagatcta
     1201 tagagaccag aaggaaatct tgaaggacga tatcgcatgc atgcttctgc atacggacta
     1261 tcacaaaatg gcggaggcca cgggtgcgac aggatttctg attgcgcgcg aatcttctgt
     1321 tgcttctggg ctcactgctg ctcgcaaagc cgcgcaaaag aaaagtccgg ttctcatcaa
     1381 cgcaatcatt gatatttcag actttcgcaa gggttcgatt tctatgtaat tatggaggat
     1441 tcccatgcat ttcccctgga caaaacacta tgacgcgcat gttcctccgg ttctggaata
     1501 tccggattct accattgggg aacttttcga tgaatccgtt cgcaaacatg gaagcagaat
     1561 cgcggttcaa ttcatgggca agaagatctc ttacgactcg atggaaaagg atgccgcatc
     1621 tctggccgtt catctttcta gcaaaggcgt gcggccaggc gatcgcgttt tcttgcttct
     1681 cccgaatact cctcatttcg tggcggccta ctatgcagtg atgaagattg gcgccgttgt
     1741 tgtgcccgca agtccgctcg acacagctcc tgaaattcag tacaagattc gcaattcagg
     1801 cgcacgcgtt gcggtgttcc tggatctttt gttcgaatcg ctcgaacctt ctttgccact
     1861 gctcgaagct gcggtcggat gcgatctgtc tgattacttg ccgtttccga aaaactattt
     1921 gttcccgatc aagaagcgca gactcgggag aaaaatgccg ccttacaatg agaaggtgat
     1981 tcggtttagc gagatcttga aggtcaagga aacctttcct tgcaaacatt ccagtccgga
     2041 tgacctggca gtcatcatct acacgggcgg aaccactgga atttccaagg gtgtgatgct
     2101 ttcgcacaga gccctcgcag tcaatatgac ccaggcgcgt acctggggtc aggtgacagg
     2161 ggatgacatc aatctgtgtg ctcttccttt ttttcacgga ttcggcatga gcatcgggct
     2221 caacactacg tttgttgcgg gcggtcgaat gctgctactc cccaaatggg atgcgggcca
     2281 ggcgatccgt cattttgaaa aggatggcgt cacgttattc gccgctgttc caacgatgta
     2341 caacagcatc ctgaatcatc cggatttttc ccgcatgaag aatgctcgat tgcgcggatg
     2401 ctatgtgggg gccgctcccg tgcccgaatc gctcaaagag gaattcttcc gtcgcacagg
     2461 aggaatcttg atcgaaggct acggcctaac ggaagctgtg actgcaaaat ccgcgaatcc
     2521 gtacattgga aagaagaagg aaaagtcgat tgggattccg tggccggata ctgagttcag
     2581 aattgtggac gagatcaccg gggaagcatc cgcgccggga gtggagggtg aaatcattct
     2641 gcgcagtcca gatttaatga tgggctactg gcagaatcca gaggcaacgg acgaagtgtt
     2701 acgcgacggg tggttgttca caggtgatat cggaagaatg gatgaggacg gatacttcta
     2761 cattgtagat cgtaagaagg atctaatcat cagtggtggt tttaacgtat atccatcgga
     2821 gattgaagag gttctgtaca gtcatccatc cgtgctggaa gcgtgcgtga tcggagtgcg
     2881 agatactcac aagggcgaaa ttccccgcgc gtatgttgtg cttcgtcagg gggagcgcga
     2941 agatgccaat gcgctgatgg aattccttgg agtgcgtttg attcactaca aagtgccgcg
     3001 ttcttttatt ttcaaagacg cactcccaaa aagtcccatt gggaaaatct tgagaaaaga
     3061 actccgtgcc gctgatccgg acgttggatc gagtagagga tgatggtaag ttaacgtgcc
     3121 tttttgttct tctttctcat gctcatcccg aatccaccaa agcgaatctt gatcttcggg
     3181 tttcgtgcga ggatggcgct ttcgagtgct ttgacttttt tttccggctg ctccaggact
     3241 actgatatcg aaatccctat gtactttcct gattctactt cgcgaaggag atcattctca
     3301 ggtaagttgc cggtatacca ggcagagatt ccccgactgc gtaaaactgc gtaatgcagg
     3361 aggctactca gatcgtgcgg atctccagga aatgtggtta caagccatcc cggtttcgcg
     3421 tgaggaggaa acttttcaat cttcgcgaat atgtatccgt gaagccagtg tgaaaatgca
     3481 tgctcctccg ggacactgag tagattttct tcccatgcct tgcctacgag aattactgcc
     3541 ggtccgataa tgtggtgcac ccatgccgag aaggacccct tgagttgctt gtcgaaaatc
     3601 ttttctacac tgtcgtaatc atgggcctgc aaaagcttta tgaatgttaa ctgttcctct
     3661 gaaaaggttg tgactttgtc cttcagcagg gcttcgcgtc ccagagccat gatctttttg
     3721 agcttctgcg attgatcgag tcgcatctgg atgcttttga gtacaaagta atcatcattc
     3781 gaataatgcc agtaaccatt cggtgtgcga aacggtcgta gaatgttgta tctttcctgc
     3841 cacttacgaa tggcgtgcga agcgattcct gtcattttac tcagatcttt gatcagatag
     3901 tccattgagc ttcagtatac agttgctgcg tttctttgtc taatgttttt agaatcattg
     3961 gacaaaaaat ggatggcgat tgatgatcct tctgcctcct tatggacatg aagcggctca
     4021 cgtacatcag caacttttcg caacccctcg cgcgaaatga aattgaacga atcggcgagg
     4081 tgtctgtcag gaacaatgcg cgcgacggcc ttacaggagc cctattctgt ttccatgatg
     4141 tcttctatca ggtcatagaa ggcgaagagc aggccattga gcgctgttat tcgaggattc
     4201 tgaaagactc caggcacaaa gatatcttct gtttgaaagt ggagcagggg gtttcgtctc
     4261 gcatgtacgg agactgggcc atgaaaaccg tgattctgga ggaatcgggc gaagcaatcc
     4321 tcgtggctct ccgaaacatg atggacggtc tctcttccac gcatcgtata ctggagcgtt
     4381 atgcgccagc ggagattctg caaaccatgc agaagggcag aaatcctttg agtgaaaact
     4441 cgagcatgac ggagagagtc gtgatatttg cagatattct ggcctccact actttaacgg
     4501 aggttctgcc ggccgaaact atggcgctgt tgctggcaga ttactatgag attgcgaatg
     4561 cagccattgt cgaatccggc ggcactattt caaagctcac tggagatggc ttgatggctc
     4621 atttcgaaac gggcaaggcg gactcggccc tggacgcgag cattcgcatc attcatagaa
     4681 tcaacgacct tcgaagccgc gcagaatctc atgaccctcg aaagttcctg tactccggaa
     4741 ttggaatttc cgcgggccgg gtgctggaag gaagcattgg ttcggatctg cgaaaggatt
     4801 acactttgct tggcgacccg gtgaattccg cggccaggct tgaatctgta accaggaaga
     4861 ccggtcatgc gattgtattt gattcgcgct ttcgctcctt attgcgcaag gaacgcacac
     4921 tcaagaagct cgggcgttat ttacccaaag gaaaaacgga tcaactgcag atatttacga
     4981 ttccagaaga cgcaatcaaa ttccacataa cgtcagagag tctacgcaaa tccattcaaa
     5041 gcctctctga accggcacgc ttgtgaaatt gaatctcaac tagtgctggt tttcggttcg
     5101 tagcgcgttc cttcgatctt tttcgttgcc atcctcggaa agatcgattc tgtgaaccag
     5161 tgcgcactgc agcagagcaa caggcaactt atcgtctgcg tctacgggct tccgttgtgt
     5221 ccatcgtggt tggctttctt gtatgcttgt tgaagtttgc agggtttctc actacaggtt
     5281 cctacgcgat cctttcggat gcactcgaaa gtatcataaa tgtggctgca gccagtttcg
     5341 ccgcatactg cattgtgcag ggtgcaagaa aggcggactt tgaccatcca tacggtcgcg
     5401 gtcgcctgga atatttttca gcaagccttg agggtgctct cattttgctc gctggcgtta
     5461 tcattttgaa tgagtctatt ccaaagttgt ttgccggtaa tgtagtgggc aggatggatc
     5521 tgggtctaat gttcacggga gtgggcaccg tcgcgaacgg aatcctggcc gctttcttga
     5581 ttcgttctgg caagaaacat cacagcatgg ctctagaagc ggacggccat cacgtatggt
     5641 ctgacgttgt ttcaagtatt ggtttgtttg ttgggttggt tctggtggcc atttcaaaac
     5701 tgccgtggtt ggatccattg cttgcgtgta tcatggcact atggattttg tggtccgggt
     5761 ttcgaattct tcgtcagagt ttctttcagc taatggatcg tgccagtccg gatgtggtta
     5821 cgaaagtgac gagcgcgatt gcggaattgc gaaagccgga aatgattcat ccgcatcgca
     5881 tgcggttacg cgaatcaggc ccgtatgttc tggtagattt ccacatgatt gtgccgcgct
     5941 ttttcacagt gaggcaattg cacgatctgg aagaacacat tcatcggaat ctggagagca
     6001 ttcttgacag acctgtggat ctgatgctcc acaatgatcc atgcacttcg aataactgtg
     6061 gttattgttc gatgaagaat tgcagggttc gcaagaagaa gcaaacttca caacttaaac
     6121 tggatcccca acatttgctg gaggatattt cgcatccagt acacacgcgt gatcggaggg
     6181 gggactaggt actatttctg ttcctggcct cggacccacg ttcctgtgcg agacttccca
     6241 tcctgcgccg tgtaagttcc gtgtccgtgc ggttggtcgt tttgaaattg gccttcgtat
     6301 ttgcgtttgt ccgcgtacga gtagacgccg gtgccttctt tgaaattgtt tttgaaatct
     6361 cccgtgtact ggtccccgtt cgcgtactta taaatgcctt ttccttcctt cttcccgttt
     6421 acgaattcgc cagtatagac gccaccgtcg ctgtgagtca gagtcccttt cccttgaaaa
     6481 actccgttca caaattcccc ggtgtaattg cttccgtcag gcagagtgct ttcccctttg
     6541 ccctgaagct gtccatctgt gaatgatcct gtaaatgtag tgcggtctgg cctggtcaga
     6601 gttcccttca caaacttatt gctttcaaat tggccctgcg ttctggttcc atctggcagg
     6661 gtttctgttc cctctccatt tagttttcca tttacgaagt tgccctcaag ctttctgccg
     6721 gttttcagca aaaagacgcc tttgccttcg atcttgttgt ccttgaattc tccagtatat
     6781 acgccggacg gcagagtgta aacaccctgg ccgtggaaat tgccattctt gaattcgcct
     6841 tcgtatcgcg agccattggg aagctgcaga gttcccggac catttttgca atcccctcgc
//
LOCUS       DAORFF010000146         6873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5648_length_6873_cov_3.307128, whole genome shotgun sequence.
ACCESSION   DAORFF010000146 DAORFF010000000
VERSION     DAORFF010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5648_length_6873_cov_3.307128"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PKK76_07595"
     CDS             complement(<1..468)
                     /locus_tag="PKK76_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68372.1"
                     /translation="MRRVIILIAFAALAAPSALFAQLYTPPGYGAGQSVQGMGFGLDL
                     GYETVDDDIYVTVAPLLELPFFFDIKLGVQIPLEVLAYDRDPKSGKKTGSLRTGTFDD
                     TSDYIKVIKYARRGTHLYYNPGDAFNWSFFYGQMTDGYIGHRTIVHRYVNNYDP"
     gene            complement(521..2128)
                     /locus_tag="PKK76_07600"
     CDS             complement(521..2128)
                     /locus_tag="PKK76_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HNL68373.1"
                     /translation="MNFRLLSEYPELSSEERSLCHMLIQVETPPVTDSPTRRPLVVAI
                     AIDKSKSMYGEKMQATLEAASSLVNWLTRHDQLAIIAYDTNVEVVQPLTPLSDKFSVI
                     KKLENVRVGTATNLSGGWLQALRSVEAADVENAFRRVILLTDGMANTGIVSTPELVKI
                     AEDHFSRGISTTTIGFGRDFSELLLRDVAKAGGGNFYFIDGPEQAQEVFFREFGDIAA
                     LFGQGLEIKLKTSPGVQVKEILNDMPFQMQDDGSVILRPGDLRSDDLRNIVVVLEIDG
                     KVAAPYSEGLLGAELSFYNLVHGSRLERFDAGVAMNFKARPSDGYNKTVRLEALTAAS
                     ARAMIEASRVSGERDLSAAKQILGRMVRRLQENEATEPDQVRKLIRRLEEMERNLEEN
                     MNLARKNMMAGGAELGRITSGEYYFSGTAHDKVAELSLSGQLDLYKCPDLKGDMRRLM
                     EQGYRFVVFDLTDLSYVDSSGIGTLIQVSNWMKKRGGLMVLVNVQGSVEKIFQLSKLD
                     EFFAFRDTVSEARQFLEELIAQGRQGR"
     gene            complement(2185..2844)
                     /locus_tag="PKK76_07605"
     CDS             complement(2185..2844)
                     /locus_tag="PKK76_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68374.1"
                     /translation="MRRLLIVIWIVLCAPLIAEKEDESKLAYESGIAYSMAGDAAKAK
                     AQYEKAASVKGDFSDLARVELLRLQARDGKTLIAPLTQMLSQIKDQTLQEQAYLALAG
                     SLKESRRYQDSIDVCMLLVGRMPESELADDALLLSARIFFDLGQFDSARSQAEQIVAR
                     YKKSNSLDSARLLLAELYLIHDENYSPALACKFYASAARDSQAILTPVDSNLSRICSE
                     R"
     gene            complement(2844..3467)
                     /locus_tag="PKK76_07610"
     CDS             complement(2844..3467)
                     /locus_tag="PKK76_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68375.1"
                     /translation="MNSLFALIDRMTAKLPPNVLRLIRLGAILVWTVGATVVVFFSWK
                     KGQDATPAQGQDLSIATIKERVQKEENRRIRGDVTVPDLGEFLPESSSPDLPRQRKEN
                     HKQGLSGVDDRLIDPENPIEKPSALPPFLGEDARGETPLRLPDTRPREDAKRDDMRRS
                     DRSREGGPREDKPRENLRDAGRSNDRRSDMPAPARRDRQKPDLMPVE"
     gene            complement(3507..4697)
                     /locus_tag="PKK76_07615"
     CDS             complement(3507..4697)
                     /locus_tag="PKK76_07615"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNL68376.1"
                     /translation="MKISWKLLLAPVFALFVLSYVDKAFLLPEVRDRFLQPGGMVYYK
                     QRERQIDRMREYAKAKPPDIHLAVVLGDSRSFAIGNLPAQYIGRKNWKIFNFAGPQAN
                     PAYFYYLSRRLFTGLRRPGYMILEVSPESFNRNTGILGTTILNHGIAGGYMEPMSTMI
                     PQMDKEAYNDTRAFAFYGLPFSLREAILRVRGSISPEKKKSGSMFDLIPIEQMEKGFQ
                     AIYGPAVEWQSGLMMSMKNAEIHNLDLYSMEQSHEVKFLNLMNGAQYAWFGTATDAEL
                     KSDTDRIAGIYLKRFQVSEEQVVFFERTLDAARAAGVKVIVFWPQVNPYLETRLNADP
                     RFAILKKRIADESKDRGAVVLDMSAQEETRCKNFYDASHLSIQCFPAITGFLLDRLEK
                     VPSN"
     gene            complement(4705..6147)
                     /locus_tag="PKK76_07620"
     CDS             complement(4705..6147)
                     /locus_tag="PKK76_07620"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNL68377.1"
                     /translation="MLFVTAQFLVFLVCTVVIYWVLPPKFRIWFLTAASLFFYATWNP
                     RFMLHFLVVVGVNWSVIELWRVYRKNWIFYVLQLANLCNLAFFKYFYFFADIIGRSAG
                     ISWLEQPAFQDSQKALGYEILLPLAISFYTFQIMSYGFDIFRGVYTEKNSLLELLLFK
                     AFFPQLIAGPIMRSAELLPQIRQCRTEGLTLDQNSFYKGLWLCMIGVVKKVLIADRLM
                     EYLGPIIGTKDPSVYAPLYLWLAVIAFAAMVYSDFSAYSDLARGFGLLLGFQIPVNFK
                     APLFFISVTDFWRRWHITFSRWLRDYIYIALGGNRVSKARLYFNFIFTFFIGGLWHGA
                     AYTYVVWGILLGVMLSIESFMESKGIPEFPVTWWGRILRRLLGHLMFWPTTVFFMGTS
                     WTWCMHALSSMANLPRLVVADGLEPMHVETALYAMAGVLVFHLFDEYPERFAKLRRAE
                     RILVPAGGLALILILSQFTGGQKDFFYFQF"
     gene            complement(6151..>6873)
                     /locus_tag="PKK76_07625"
     CDS             complement(6151..>6873)
                     /locus_tag="PKK76_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNL68378.1"
                     /translation="KPSAEKEVRINRYLASAGIGSRRDVEDLVLKGRVEINGRKVSDL
                     FERVSAGDLVRVDGKLIRPLDHKYYIFNKPVGVVCSSRRFGDEEKIIYDYLPEGLRDL
                     KYAGRLDQDSRGLVVLSNDGDFIQAITHPSHRITKKYLVTAEYLPPENVLRRTFVKGI
                     EHDGDILKAISAHIRDRHQGLVEVVLGEGKKRQIRRMFESVGSNVVDLFRTTVGFCDI
                     EKEPLKEGELRAISPEQLLYGE"
BASE COUNT         1775 a         1791 c         1663 g         1644 t
ORIGIN      
        1 tggatcatag ttgttcacgt atctatgaac aatcgttctg tgaccaatgt agccgtccgt
       61 catctgcccg tagaagaaag accagttgaa tgcgtctcca ggattgtagt aaaggtgcgt
      121 tcctcgacga gcatacttga tcaccttgat gtagtcggac gtgtcatcaa aggtcccggt
      181 acgaagagat cctgttttct ttcctgattt tggatcgcgg tcgtatgcga ggacttccag
      241 tgggatctgg actccaagct taatgtcaaa gaagaacgga agttccagga gaggcgctac
      301 cgtgacgtaa atatcatcgt ctacggtttc gtagccgagg tccaggccaa agcccatgcc
      361 ctggacgctc tggcccgcgc cgtatccagg cggagtgtag agctgggcaa acagcgcaga
      421 cggagctgcc agtgctgcaa aagcaatcag gatgatgacg cgacgcaatt cttcctctag
      481 tatcttcgat acggaaagta cgggcaaaaa atgaagtaaa ttaacgaccc tgacggccct
      541 gggcgatcag ttcttccaga aattgccgcg cttcagaaac cgtatcgcga aatgcgaaga
      601 actcatccaa ttttgaaagc tggaagatct tctcaacact gccctgaacg ttgaccagaa
      661 ccataagccc tccccgcttc ttcatccagt tagaaacctg gatcagggtg ccgataccgg
      721 acgaatctac atacgagaga tccgtcagat cgaacacgac gaagcgatat ccttgctcca
      781 ttagacgacg catatcgcct tttagatcag gacacttgta aagatcgagc tgccccgata
      841 gactgagttc cgccactttg tcgtgggccg taccgctgaa atagtattcc ccggaggtga
      901 ttctccccag ttccgcgccg cctgccatca tgttcttgcg ggcaaggttc atattctctt
      961 ccagattccg ttccatctct tccagacggc gaatgagttt ccgcacctga tctggctcag
     1021 tggcctcatt ctcctgaagg cgacgcacca tacgaccaag gatttgtttg gcggcagaaa
     1081 gatccctttc tcccgaaacc cgcgatgctt caatcatggc tctggcactc gctgcagtca
     1141 aagcttccag gcgaacggtc ttgttgtatc cgtcagaagg cctggccttg aaattcatcg
     1201 cgaccccggc gtcaaatcgc tccaggcgag atccatgtac aagattgtag aaagaaagct
     1261 ctgcccccag caagccttcg ctgtatggtg ccgctacctt tccgtcgatt tcaagaacga
     1321 ctacgatgtt gcgaagatca tccgagcgaa gatctcctgg gcgaaggatg acagaaccat
     1381 cgtcctgcat ttgaaacggc atatcgttta gaatttcttt tacctgaacg ccgggggaag
     1441 tcttgagttt gatctcaaga ccctgaccga agagtgccgc aatgtctccg aattcgcgga
     1501 agaaaacttc ctgcgcctgc tctggaccgt ctatgaaata gaaattccct ccgcctgcct
     1561 ttgccacatc gcgcaaaagc aattcagaga agtcgcgtcc aaatccaatg gttgtagtgg
     1621 agattccgcg cgaaaaatga tcttccgcaa ttttcacaag ctccggagta gaaacaattc
     1681 cagtgtttgc catgccgtct gtaagcaaga tcacacggcg aaatgcgttt tcaacgtccg
     1741 ctgcttccac agaacgaaga gcctgaagcc agcccccgct caaattcgtg gcagttccca
     1801 ctcttacgtt ttccagtttc ttgatgactg agaatttatc cgaaagagga gtcagaggtt
     1861 gcacgacttc tacgtttgta tcgtatgcaa tgatggcgag ttgatcgtga cgggtgagcc
     1921 agttcaccag agaagaggcc gcttccagag tagcctgcat tttttcgcca tacatggatt
     1981 tacttttatc gattgcaatg gccacgacga gcggacggcg agtaggcgaa tctgtgacag
     2041 gtggtgtctc cacctgaatg agcatgtgac agagacttct ctcctcgctg gagagttccg
     2101 gatactcgga tagtagacga aagttcatat cacctgcccc agacttcaat ttccgggggg
     2161 cgaacaaaag atcaagtatt tccgtcagcg ctcagagcaa atacgagaca aatttgaatc
     2221 gaccggagtc aaaatggcct gcgagtcgcg cgccgcagat gcgtagaatt tacatgccag
     2281 agcgggcgaa tagttctcgt cgtgaatcag ataaagctcg gccaggagaa ggcgggctga
     2341 atccagggaa ttggacttct tgtagcgagc cacgatttgc tccgcctggg atcgcgccga
     2401 atcaaactga cccagatcaa aaaagattct ggcagaaagc aatagagcat cgtctgccag
     2461 ctcactttcc ggcatccggc ctacaagcag catgcataca tcgatggagt cctggtatct
     2521 tctagattcc ttcagacttc cggcaagcgc aagatacgcc tgctcctgga gggtctggtc
     2581 ttttatctgc gaaagcattt gagtgagcgg agcaatgaga gtcttgccat cgcgagcctg
     2641 cagccggaga agttctacgc gcgccaggtc tgagaagtcg cccttcacgg acgctgcttt
     2701 ttcgtactga gcttttgcct tcgctgcgtc acctgccatc gaatatgcaa tgcctgactc
     2761 gtaagcaagc ttactttcat cttctttttc agcgatcaac ggagcacaga gaacgatcca
     2821 tattacaatc aaaagtcgtc gcatcactct accggcatca gatctggctt ctggcgatct
     2881 cttcgcgcag gtgcaggcat gtcgcttctc ctatcattgc ttcgacccgc atcgcgcaaa
     2941 ttctccctgg gcttgtcttc gcgaggtccg ccttcgcgcg acctgtcaga tcgccgcata
     3001 tcgtcacgct ttgcatcttc gcgcggtctt gtatccggta ggcgaagagg agtttctccg
     3061 cgtgcgtctt cgcccaggaa tggaggaaga gcagaaggtt tttcaattgg gttttcagga
     3121 tcaatcaagc gatcgtccac tccagagagt ccctgtttat gattttcttt tctttggcgc
     3181 ggcaggtccg gtgaggatga ctccggcagg aattctccca gatctggcac tgttacgtcc
     3241 ccacggatcc ggcgattctc ttccttttgc acgcgctctt tgatggtcgc aatggataga
     3301 tcctgaccct gggctggagt cgcatcctgt cccttcttcc acgagaagaa cacaaccaca
     3361 gttgcaccca cggtccagac caggatggcg cccaggcgaa tcagcctgag cacattggga
     3421 ggcagttttg ccgtcatccg gtcgatcagg gcgaacaatg agttcatgat ggtattatcg
     3481 ttaagattta gaagagtgtg gacaatttaa ttagatggaa ctttctccaa tcggtcgagt
     3541 aaaaagccag taatcgcggg aaaacattga atggataggt ggcttgcgtc atagaagttc
     3601 ttgcagcgag tttcctcttg agcgctcata tccagaacaa ctgctccgcg gtctttagac
     3661 tcatctgcga ttctcttctt gagaattgca aagcgcggat ccgcattcag gcgagtttcc
     3721 agataaggat ttacctgagg ccagaacaca atcaccttca ctccagcagc cctggccgca
     3781 tcgagtgttc tctcaaaaaa cacaacttgc tcctctgaaa cctgaaatcg tttcaaatag
     3841 atgcccgcaa ttctgtctgt gtctgattta agttctgcgt ctgttgccgt tccaaaccat
     3901 gcatactgcg cgccgttcat aaggtttaga aactttactt catgagactg ctccatgctg
     3961 taaagatcca ggttgtgaat ctccgcattc ttcatggaca tcatcagacc gctctgccat
     4021 tccaccgcgg gtccgtagat tgcctggaag cccttttcca tttgctcgat gggtatgaga
     4081 tcaaacatac ttccggattt ctttttctcg ggcgaaatcg atccgcgaac gcgaagaatc
     4141 gcttcgcgaa gtgaaaaagg cagaccgtaa aaagcaaatg ctcgcgtgtc attgtatgct
     4201 tctttgtcca tctgcggaat catagtggac attggttcca tatagccgcc agcaattccg
     4261 tgattgagaa tcgttgtgcc cagaatgccc gtgttcctgt tgaaagactc tggggaaacc
     4321 tcaaggatca tgtaacccgg tctgcgaagg cctgtgaaaa gtctgcgaga aaggtagtag
     4381 aaataagccg gatttgcctg cggtcctgcg aaattgaaaa tcttccagtt ctttctgcca
     4441 atgtactgcg ccggcaaatt tccaatcgca aatgatcttg aatcgccgag tacaactgca
     4501 agatgaatgt ccggcggttt tgccttcgca tattcgcgca tgcgatcgat ctgcctttcg
     4561 cgctgcttgt agtataccat cccgcctggc tggaggaatc gatcgcgcac ttcaggcaaa
     4621 agaaacgctt tgtctacgta ggatagcacg aagagtgcga aaacaggagc gagtaataat
     4681 ttccagctga tcttcatgat tacctcagaa ctgaaagtag aagaaatcct tttgcccgcc
     4741 ggtgaattga gacaaaatca aaatcaatgc cagtccgccg gctggaacca gaattcgctc
     4801 tgcccggcga agcttcgcga aacgctcagg atactcgtca aacaaatgaa agacaagcac
     4861 acccgccatt gcatagagag ccgtctccac atgcatgggc tccagtccat ccgccaccac
     4921 caggcgtggg aggtttgcca tggaactcaa cgcgtgcata caccaggtcc acgaagtgcc
     4981 catgaagaaa acagttgtgg gccagaacat caaatgaccg agcaaacggc gaaggatcct
     5041 cccccaccac gtcacaggaa attctggaat ccctttggac tccataaacg attcaatgga
     5101 caacatcaca ccgagcaaga tgccccacac tacgtatgtg taagccgcac cgtgccagag
     5161 tccgccaatg aagaaagtga agatgaaatt gaagtacagc cttgcttttg aaacacgatt
     5221 tccacccagt gcgatgtaaa tatagtctcg caaccatctc gagaacgtta tatgccatct
     5281 gcgccagaaa tcggtgacag aaatgaaaaa caacggagcc ttgaaattca ccggaatctg
     5341 aaatccaagt agaagcccga atccgcgcgc aaggtcagag tatgcggaaa aatcagagta
     5401 caccatggcc gcaaacgcaa tcaccgcaag ccatagatac agaggagcat agacagacgg
     5461 gtctttcgtg cctatgatcg gcccgagata ctccatgagt ctgtctgcga tgagtacttt
     5521 cttcacaact ccgatcatgc ataaccagag acctttgtag aaagaattct gatccagggt
     5581 cagaccttct gttctgcact ggcgaatctg aggaagaagt tcggcagatc gcatgatggg
     5641 gccagcgatg agctgcggga agaaagcttt gaatagaaga agttccagca gactattttt
     5701 ctccgtgtat actccacgaa agatatcaaa tccataggac atgatctgaa atgtgtagaa
     5761 gctgatggcc agggggagaa gtatctcgta gccgagcgct ttttgcgaat cctggaaggc
     5821 tggctgctcg agccacgaaa tcccggccga tcgtccaatg atatcagcga aaaagtaaaa
     5881 atacttaaag aatgccaggt tgcagagatt cgccagttgt agaacataga agatccagtt
     5941 cttgcgataa acgcgccaga gttcgatcac actccagttg actccgacca ctaccaggaa
     6001 atgcagcatg aaacgtgggt tccaggttgc gtagaagaaa agagaagcgg cagtgagaaa
     6061 ccagatgcga aactttgggg gtaaaaccca gtagatgaca accgtacaga caaggaaaac
     6121 caggaactga gcggttacaa acagcatcga ttattctccg tataaaagct gctcggggct
     6181 gatcgcgcga agttcacctt ctttcaaagg ttctttctca atgtcgcaga atccaacggt
     6241 agtgcgaaat aggtccacaa catttgagcc tactgattcg aacattctgc ggatttgcct
     6301 cttctttcct tcccccagaa ccacttccac aaggccctga tgacgatccc ggatatgcgc
     6361 agaaatagct ttgagaatgt ccccatcgtg ctcaattccc tttacaaacg ttctgcgcaa
     6421 aacgttttca ggcggaagat attctgcggt tactagatac ttcttcgtga tgcgatgcga
     6481 aggatgtgtg atagcctgga taaaatctcc gtcgttggaa agcactacaa gccctctaga
     6541 atcctgatcg agtctacccg catacttcaa atcgcgcaaa ccttcaggaa gataatcgta
     6601 gatgatcttt tcttcatccc cgaatctcct cgaggaacag accacaccca cgggcttatt
     6661 aaatatgtaa tacttatgat ccagaggccg gatcagttta ccgtctactc gaaccagatc
     6721 accggcggaa acgcgttcaa aaaggtcgct caccttcctg ccgttgattt ctactctgcc
     6781 tttgagaacc agatcttcta cgtcgcgtct gctcccaatt ccggcggatg ccagataacg
     6841 gttgattcga acttcctttt ccgccgaggg ttt
//
LOCUS       DAORFF010000147         6857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5663_length_6857_cov_3.523817, whole genome shotgun sequence.
ACCESSION   DAORFF010000147 DAORFF010000000
VERSION     DAORFF010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5663_length_6857_cov_3.523817"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PKK76_07630"
     CDS             complement(<1..506)
                     /locus_tag="PKK76_07630"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002186319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HNL68379.1"
                     /translation="MKKSTLLEQDLTEELFQEFSQAPELGVDCEMMGLNPARDRLCLV
                     QIGKEGGPYALIQIDEQAGAPRLKSLLTNPEITKIFHFARMDCLFFKARLGIDVKGIF
                     CTKLASRLVRTYTDRHGLKELVREFTGEQLDKTITSSDWGNPKLSPEQLKYAEEDIKY
                     LFKIKRKV"
     gene            complement(511..2028)
                     /locus_tag="PKK76_07635"
     CDS             complement(511..2028)
                     /locus_tag="PKK76_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNL68380.1"
                     /translation="MCIGALWLLRCATTAEATATARKPDIDWTTRYGVLLVDPDEGKY
                     LASRKGSHLADTRPDGTAVTVPAEEAKKRRDRGYRTIQDPPFRYIDPEISTRRKSNDP
                     EVWWSGYKDEEIVEKIVRGLATAYPGDATLFDIGKTHRGRHVLALRLGSRAGIEQRPS
                     FLFDGGHHGNEPLSVEYVLDVMRTLLSSVSPVPGAPVSPVSLSYLEQRQMREYLDNFQ
                     IWCVPLVNPDGMNVFWNRSQIAGRRNGRELDGVDLNRNYPFYWNSGSRNASSDRPGST
                     FYRGPSPGSEPETKAMMNLADRQRFVVSLSYHTFATKVLVPYTTDGAISPVPMTGWRF
                     ANELVRAGRSNRPDKNYIAARNLYPVDGTDQDWLHNTYGTLAFIVEGSYQTPEFATGG
                     LASIRGMRPLSLRALSLYANGPTIQVKVLDHSGKPARVRIRRLDQALLEQEHFETHPL
                     TGTYDFILDEPGDTAIQVVFQENGKLRQILRTVHCSTGVCPVTIRLTSDTDNLPW"
     gene            complement(2043..3311)
                     /locus_tag="PKK76_07640"
     CDS             complement(2043..3311)
                     /locus_tag="PKK76_07640"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNL68381.1"
                     /translation="MRFLILFASSTMLSAQKLLSVAALGLFAFCGKSKEVPPINPTKV
                     FTQVEAAITTTYFSGDPAPPLEKLYGNAVAALDAKETPDMKGTIKAIESQGPDKVRER
                     TYRALNAFLSELPGGNDFTRAEGLLMSEDKSRQAGIGILLMQEGPGKFIVLDTLESSP
                     AQAAGVKPGSVVKSIDGLEVANMDLEEVAGRIRGAESTDVRLGLLGSEPTIKRSKLNF
                     INYSNATWLLTTGGQVEIVALRTAVPGTATNLEQFMRQMGKRSAVILDLRKLQSGDFS
                     ECFKIADLLVGKRKMGELVQKNGAIPMESTADSLFTGPIYVILGKHSSSAADVVAMAL
                     RSSLEVNLIGVDRPGLAYFAENIPLDGGIVLKLTKGFVADERSIPLPKRGLKMDVNID
                     DYLPLEPPGDKPSEADPAHRQLRTMLGLAR"
     gene            complement(3346..3579)
                     /locus_tag="PKK76_07645"
     CDS             complement(3346..3579)
                     /locus_tag="PKK76_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68382.1"
                     /translation="MFRIALVTALALGMACGNTFETHACHRDCSNRYNSCLTTMVLLN
                     VPTNQSPAFNTAGVLSCNTDHDNCNATCSQLGD"
     gene            complement(3572..4720)
                     /gene="aroC"
                     /locus_tag="PKK76_07650"
     CDS             complement(3572..4720)
                     /gene="aroC"
                     /locus_tag="PKK76_07650"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNL68383.1"
                     /translation="MSSVAGTLFTISTFGESHGPAVGVVLDGCPAGFEIDLEELQAEL
                     DRRKPGQSHLTTSRQEPDRVEILSGLFEGRTLGTPIAMMVRNVDMRSQDYEGFSQAYR
                     PSHADFTYHAKYGFRTPHGGGRASVRETIGRVAAGAIARQILRKELGIETIAWVESIG
                     NIDSQAIKNPPQSREQVDSNLVRCPNEIAAKKMVALVEEARAKKDSVGGTLGVIVRNV
                     PPGLGDPVFDRLEADFAKASLSIPACKGFESGSGFEGTRLFGSQHNDSFIPKENPREG
                     ATETMPAPNVETKTNFSGGIQGGISNGMPILCRLAFKPTATISQPQDTVNDRGEATEL
                     AAKGRHDPCVLPRAVPIVESVFNLVLMDAYLRQRAQTPEWWLRYRKNV"
     gene            complement(4756..5727)
                     /locus_tag="PKK76_07655"
     CDS             complement(4756..5727)
                     /locus_tag="PKK76_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNL68384.1"
                     /translation="MLPHNKLISSLGGAKQVYIQTHNFPDHDAVATAYGLQYFLKRAG
                     IQANVIYEGEVQRDSLKRMIQELNIDVRPASRYALTAEDKIIVVDGCKGNKNVTDLIG
                     DEVAVIDHHQVTAPDDVKFADIRPEYGACSSIIYEYLNTQEGQIPQNVATALMVGINM
                     DTSLLTRSVSKGDLLAYTELYGLADMPLVNSILINYIQTKDLAFYKTALDRVRIKETL
                     AFCYFPDGCNQNLMGILGDFFMSLQEIEFVALCAKNDGKINFSLRSENRSWNASKVIQ
                     EVLEGVGFGGGHNDRAGGIIQDAALFHEEEIYKKVVRALKLPDIAIF"
     gene            complement(5763..6677)
                     /locus_tag="PKK76_07660"
     CDS             complement(5763..6677)
                     /locus_tag="PKK76_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNL68385.1"
                     /translation="MSIIGRAALAYRNFQIHRRILSSGMKSQFIPFEGIRFHMYAHEN
                     GKPPLFLLHGFLDSSKTFRKLFPELIRKFDVYAIDLPGFGSTRMPEVRELWRIDSIAR
                     QTARFILHILKQRPPQAPQRTNKVDQSPQILTHSMGGLIAMHAVEYLQDHDLEVPFKR
                     MHFVAPGALRFSESDRDAHRKRFYPTSTDEIRELLGELFHETRTDLPDFLLRGLLEQW
                     SHKGYEYLAENTIEEEDSVFYPSGKRIKLSPEIILYWGREDRIVNLQYGKLVRMTLRC
                     RLIEMQGGHCVQTENPDELMRAFLANCK"
BASE COUNT         1512 a         1751 c         1761 g         1833 t
ORIGIN      
        1 tgaaccttgc gcttgatttt gaagaggtac tttatatctt cttccgcata cttcaattgc
       61 tctggtgaga gttttgggtt gccccagtcc gaagatgtga tggtcttatc cagctgttct
      121 ccggtgaatt cgcgaacgag ttctttgaga ccgtgtctat ccgtgtacgt gcgaaccagc
      181 ctggacgcca gtttggtgca gaagattccc tttacgtcaa tgccaaggcg tgctttgaag
      241 aacaaacaat ccatacgtgc gaagtgaaat atctttgtga tttctggatt ggtgagcagc
      301 gatttgagtc tgggtgctcc ggcttgctca tctatctgga tgagtgcgta cggtccgcct
      361 tcttttccaa tttgaaccag gcagaggcga tcgcgagctg gattgagtcc catcatttcg
      421 caatcaacgc cgagttccgg cgcctgggaa aactcctgga acaactcttc tgttagatcc
      481 tgttccaata gcgttgactt cttcatgaca ctaccaggga agattgtctg tatccgaggt
      541 caaacggatt gtgacggggc aaactcccgt cgagcaatga acggttcgca ggatctggcg
      601 tagcttcccg ttttcttgaa agactacctg aatggctgta tcgcccggtt catcgagtat
      661 gaagtcgtat gtgccagtca acggatgggt ttcaaagtgt tcctgttcga gcaatgcctg
      721 gtccagtctg cggatccgga ctcgcgctgg ttttccagag tgatcgagga ccttcacctg
      781 gatggttggt ccgtttgcat agagggagag cgcccgcaga gacaggggtc tcatcccgcg
      841 aatgctggcc agtccgcctg ttgcaaattc gggagtttga taggaacctt caacgatgaa
      901 ggcgagagtg ccgtatgtat tgtgaagcca gtcctgatct gttccgtcca cgggatagag
      961 atttcgcgcg gcaatgtaat tcttgtccgg ccgattactt cttccggcac gcacgagctc
     1021 atttgcgaat cgccatccag tcattggcac aggagagatg gcaccgtccg tagtataagg
     1081 cacgagcact ttggtggcga acgtatgata cgaaagagag acaacaaatc tctgtctgtc
     1141 tgcgagattc atcattgctt ttgtttccgg ttccgaccca ggtgaaggac cgcgataaaa
     1201 ggtgcttccc ggtctgtcgc tcgaggcatt gcggcttcct gaattccagt aaaatggata
     1261 attgcgattc agatctacgc cgtccagttc gcgaccgttt ctccttcctg caatctgcga
     1321 tcgattccaa aagacattca tcccatcagg attgacaagt ggcacgcacc agatctgaaa
     1381 gttgtccagg tactcgcgca tttgtcgctg ttccagataa ctcaaggaaa caggcgatac
     1441 gggtgccccg gggacagggc tcaccgaaga caagagagtc ctcatgacat cgagaacata
     1501 ttcaacagag agtggttcat ttccgtggtg accgccatca aaaagaaaag atggtcgctg
     1561 ttcaattcct gcgcgagatc caaggcgcag tgccagaaca tgacgacctc tatgtgtctt
     1621 tccaatgtcg aataacgttg catcgcccgg ataggccgta gccaggccgc gcactatctt
     1681 ctctacaatt tcttcgtctt tatagccgga ccaccagact tcaggatcat tgctctttcg
     1741 ccttgtggat atttctggat ctatataccg aaatggagga tcctgaatcg ttctgtatcc
     1801 gcggtctctt ctcttcttcg cttcttctgc agggacagtc actgccgttc catctggacg
     1861 cgtgtccgcc agatgagatc cctttctgga tgccaggtat ttgccttcat ccggatcaac
     1921 caggagcacg ccgtatcgcg tggtccagtc tatgtcaggc tttcgcgcgg ttgccgttgc
     1981 ctctgcagtg gtagcgcatc ggagcagcca gagcgctcca atgcacacaa gcaagaacct
     2041 tatcaacgcg ccaggccgag catggtgcga agctggcgat gtgccggatc tgcttctgac
     2101 ggcttgtctc cgggtggctc gagcggaagg taatcgtcta tgttcacgtc cattttcagt
     2161 ccgcgttttg gaagggggat gctgcgttcg tccgcgacaa atcctttggt caatttcaga
     2221 acgatgccgc catccagagg tatgttctcc gcgaaatacg ctagccccgg acgatccact
     2281 ccaatcagat ttacctcaag ggaggagcga agagccatgg ccactacatc agctgcggag
     2341 gacgaatgct tccccagaat gacgtagata ggaccggtga atagagaatc agctgtactt
     2401 tccatgggaa tggctccatt cttttgcacg agctctccca ttttcctttt tccgacaagc
     2461 aggtcagcta tcttgaagca ttcgctaaaa tcgccactct gtagcttgcg aagatccaaa
     2521 ataacagcag accgttttcc catctggcgc atgaactgtt ccaggttcgt cgcggttccg
     2581 gggaccgctg tgcgaagagc cactatttct acctgtcctc ctgttgtcag caaccaggta
     2641 gcatttgaat agttgataaa atttagtttt gaacgtttga tggtgggttc gcttccgaga
     2701 agcccaagtc gtacatctgt gctttccgcg ccgcgaattc gaccggccac ttcctcaagg
     2761 tccatgttcg ccacttctaa accgtcaatg ctcttcacta cggaacccgg tttgactccg
     2821 gctgcctggg cgggagagct ttccagtgta tccagaacga tgaattttcc aggcccttcc
     2881 tgcatgagaa ggatgccaat tcccgcctgc cgtgatttat cttcgctcat caagagccct
     2941 tctgcgcgcg tgaaatcatt tccgccagga agttcggaaa gaaatgcatt cagtgcgcga
     3001 tacgttcttt cgcgtacttt gtccgggccc tgcgattcga tagccttgat cgtgcctttc
     3061 atgtctggcg tttctttcgc atcaagtgcg gctacggcat ttccgtaaag cttctcaaga
     3121 ggcggggccg ggtcgccgga gaaataggta gtagtaatgg cggcttccac ctgggtgaat
     3181 actttggtgg ggttgatggg tgggacttcc tttgattttc cgcagaaagc gaaaaggccc
     3241 agagcggcca cagacaggag tttttgtgct gagagcatgg tactggatgc aaaaaggatt
     3301 agaaatcgca agtgatttcc gggcggcggg aaagaacctg aaacgtcaat cgccaagctg
     3361 gctgcacgtc gcgttgcagt tgtcgtgatc tgtgttgcag ctcagtacgc ctgctgtatt
     3421 gaacgcaggg ctctgatttg taggaacgtt gagaagaacc atggtggtca ggcagctgtt
     3481 gtagcgattg gaacagtctc tatggcaagc gtgggtttca aatgtatttc cacacgccat
     3541 acccagagcc agcgcagtaa ccagagcaat cctaaacatt ttttctatat cgcagccacc
     3601 attcgggagt ctgcgccctc tgtctgagat atgcgtccat gagaaccagg ttgaaaacag
     3661 attccacgat ggggacagcc cttggcaata cgcatggatc gtgcctgccc ttcgccgcaa
     3721 gctctgttgc ttcgccgcgg tcgttcactg tgtcctgcgg ctggctgatg gtggccgtag
     3781 gtttgaatgc gaggcggcat agaattggca ttccgtttga gatgcctccc tggattccac
     3841 cggaaaagtt cgtttttgtt tccacattgg gagcaggcat tgtttccgtt gccccttcgc
     3901 gcggattctc tttgggaata aacgagtcgt tgtgctggct tccaaacagc ctcgtgcctt
     3961 cgaaaccgct cccggattcg aaacctttgc atgcagggat agagagagat gcttttgcga
     4021 agtccgcctc caggcgatca aacacaggat cgcccagacc gggtggcacg tttctcacaa
     4081 tgacgcctaa cgttcctcca actgagtctt tctttgccct tgcttcttct acgagtgcaa
     4141 ccatcttctt cgcggcgatt tcatttggac accgcactag atttgagtct acctgttcgc
     4201 gcgattgcgg aggattcttg attgcctggg agtcaatgtt cccaatggat tcaacccacg
     4261 cgattgtttc tattcccagt tccttccgta aaatctgtcg tgcgattgct cccgccgcga
     4321 ctctaccgat tgtttcgcgc acggaagccc ttcctccccc gtgcggagtg cgaaaaccat
     4381 attttgcgtg atacgtgaag tccgcgtgag aaggacgata tgcttgcgag aatccttcat
     4441 aatcctggga gcgcatatct acattgcgaa ccatcatggc aatgggcgtt ccaagagtgc
     4501 ggccttcgaa caggccgctc aatatttcta ctcgatccgg ttcctgtctc gatgtggtga
     4561 ggtggctctg gcctggtttt cgtctgtcca gttctgcctg caattcttca agatcgattt
     4621 cgaatccggc cggacaaccg tcgagtacga cacccacagc cggaccgtgc gattcgccaa
     4681 aagttgaaat ggtgaagagt gtgccggcaa cagaagacac gatcctgtat cagccgcggg
     4741 gcgggcctgt caaattcaaa agatcgcaat atcaggtagc ttcagtgcgc gcactacttt
     4801 cttgtatatc tcttcctcgt gaaaaagggc cgcatcttga atgattcctc cggctcgatc
     4861 gttgtggccg ccgccaaacc caactccttc gaggacttcc tggatgactt tggacgcgtt
     4921 ccatgatcgg ttctcactgc gaagtgagaa attgattttg ccgtcattct ttgcgcagag
     4981 agcgacgaat tcgatttctt gcagagacat gaagaagtct cccaggatgc ccatgaggtt
     5041 ctgattgcag ccatcaggaa aatagcagaa tgcaagcgtt tccttgatgc gaactcgatc
     5101 gagtgccgtt ttgtagaacg ccagatcttt ggtttgaatg tagttgatca ggatcgaatt
     5161 gacgaggggc atgtccgcca gtccgtacag ttccgtgtaa gccagcagat cgcctttgct
     5221 tacgcttctg gtcaacaagg atgtatccat gttgattccc accatcaatg cggtggccac
     5281 gttttgtgga atttgtccct cttgagtatt cagatattcg taaatgatag acgaacacgc
     5341 accatactct gggcgaatgt ctgcaaactt cacgtcatcc ggcgctgtga cctgatgatg
     5401 atcgatcaca gccacttcat ctccaatcaa atctgttacg ttcttatttc ctttgcaacc
     5461 atctacgacg atgatcttat cctccgcagt gagtgcatag cgactggccg gtcgcacgtc
     5521 gatgttcaat tcctggatca ttctcttaag agaatcgcgc tgtacttctc cttcgtaaat
     5581 gacattcgcc tggattccgg cgcgtttcaa aaagtactgc agaccatagg cagttgccac
     5641 cgcgtcgtga tcggggaagt tgtgcgtctg gatgtaaacc tgtttggctc caccgaggga
     5701 tgagatcagt ttattgtgcg gaagcattac caatgaaaaa acgcaacgag tttcaatgca
     5761 acctatttgc agttcgcaag aaacgcgcgc atcagttcgt ccggattttc cgtttgcacg
     5821 caatggccac cctgcatttc gatcaagcgg catctcagag tcatccgaac cagctttcca
     5881 tattgaaggt tgacgattcg atcttcgcgg ccccagtaca gaatgatttc tgggctgagc
     5941 ttgattcttt ttccggaggg atagaataca gagtcttctt cttcaattgt gttttctgcc
     6001 agatattcgt accctttgtg ggaccattgt tcgagtagtc cgcgcaacaa gaaatcaggt
     6061 agatccgttc ttgtttcgtg gaaaagctct cccaggagtt ctcgaatttc atccgtgctc
     6121 gtaggataaa atctctttct gtgtgcgtct ctgtccgatt ctgaaaatcg cagtgcgcct
     6181 ggggcaacaa aatgcattct tttgaacgga acttcgaggt cgtgatcctg cagatactcg
     6241 actgcatgca tagcgatcag tcctcccatg ctatgcgtca gtatttgcgg agactgatct
     6301 accttgtttg ttctctgcgg cgcttgcggg ggtctttgtt tgagtatatg aaggatgaag
     6361 cgcgctgtct gcctggcgat gctgtcaatg cgccacagtt cgcgaacttc aggcattcgt
     6421 gtggagccaa agcctggaag atctatggca tacacgtcaa atttgcggat caattcaggg
     6481 aatagctttc gaaacgtttt ggaagagtcc agaaagccat gcagtagaaa gagcggcggc
     6541 tttccattct cgtgagcata catatgaaag cgaattcctt caaacggaat gaattggctc
     6601 ttcattccgc ttgaaagaat ccttctgtgg atctggaaat tgcgataagc cagggcggcg
     6661 cgaccgataa tgctcatgat cgcctaaccc gggtattcag aatttgcaag atcgtctaac
     6721 gtccgattag ctttgctgga atcatcgaaa actcgaggtc agtctggcat gtcgttttgc
     6781 cgtccacact tgcgacacca cgtgcggtga tgcgccttgg atcgccgttt ttgtcttttt
     6841 caatttcagt aatcgtc
//
LOCUS       DAORFF010000148         6843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5685_length_6843_cov_3.873306, whole genome shotgun sequence.
ACCESSION   DAORFF010000148 DAORFF010000000
VERSION     DAORFF010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5685_length_6843_cov_3.873306"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKK76_07665"
     CDS             <1..287
                     /locus_tag="PKK76_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68386.1"
                     /translation="MVPVKAVLLRRKFVMQLYARHECWNRLGRISAEAALIALESREA
                     LTFESLAERYRKFLQDYAAFPYKIPNYQIASYLGITDVALSRLRKRMNLI"
     gene            342..1007
                     /locus_tag="PKK76_07670"
     CDS             342..1007
                     /locus_tag="PKK76_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HNL68387.1"
                     /translation="MNKTVTILMIAASVSCLTLVIYCIYGHLRITRVSQGVPIPNYET
                     EASALLIIDMQRDATEPSGRLVMNPAATDRIIENLNAVTPALRKRRTLFVMIRHEWEE
                     NWILQILTNHSLRPGTRGSEVDPRIAVKPDTIITKQTSDSFGTPALDAYLRENKISHL
                     YLSGTGANHCIRRTAEAALQRGYKVTVLRELVAGKTDAETIEGLQSMEKSGGKIASVE
                     SIR"
     gene            complement(1036..2442)
                     /locus_tag="PKK76_07675"
     CDS             complement(1036..2442)
                     /locus_tag="PKK76_07675"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNL68388.1"
                     /translation="MGAPDAATQKALDHCTSRLNHSLEELKKLVRIPGVSFDGFDPSQ
                     VRDSASAAAEHIHSSGMKNVEVIQFGDVHPYVFAEWMEHPDLPTVLLYAHHDVQPPGD
                     EKGWNSKPFDPVERGGRLFGRGTADDKVGILAHTAAISAYLNTAGRLPVNVKLLIEGE
                     EEIGSSHLAEFVRRHREKLRADCMILTDCSNIDTGIPSITTSLRGLVSVDVELRGLPQ
                     PVHSGFWGGVVPDPAIALAQLLGKLTDEEGNILIPGILDDVRPLSVAEIKEFSTLDLE
                     KIVRAGTQLPDHLPLRGTSEELAKRMWREPSLSVLAFESGSRKMVANIIQDGAWARVS
                     MRIVPDMKPQKTQDILSDFLRSHCPRGFEITITPEAAADWWTVQDLNDPIFDIAARSL
                     EKGYGQKTRFVGCGASIPFVQPLSQELGGIPAVLLGVEDPYSNPHSDNESLHLDDFRK
                     ILISQVHMLGELARYRRG"
     gene            complement(2536..5382)
                     /locus_tag="PKK76_07680"
     CDS             complement(2536..5382)
                     /locus_tag="PKK76_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004472501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNL68389.1"
                     /translation="MLSPSKPIDRYRASGSAWVASFSCSHLNVLIVCRGPIRKEAMDV
                     FESLGAGYGILLSEKDSVTYPHTIAPELRHLNDQNRIHRVADYAGANAEEKKIRIQQI
                     IDFAVKGNYTHIFSGYGFMAEDAEFVEEIEKAGIGFMGPASGVHKQAGAKDMAKKLAR
                     KLGLSVTPGLDNISALALLTKAGGEKGLEKIAKDNGIDFADAQKRAGSEGPAEVAEQI
                     LEAAYAKRAPLLSVKDLQTEASVQLNKLFSENPGKRFRLKYIGGGGGKGQRIVAKISE
                     VENAVQEVLAESKATGDADNKNFLIELNIEKTRHNEIQLLGNGQWSIALGGRDCSLQM
                     HEQKLVELSLTDELYAAEIQIAESRGRADHAKALGVDRALLARMEEQGEKFGTATGLN
                     SASTFECIISGHDFYFMEMNTRIQVEHRVTEMVYSLRFENPANKSDFFVVESLVEAMA
                     LCAAHGPRLPKPERIKRNVAGGEIRLNAQNEALQPSSGGLIEFWSPPVANEIRDDQGI
                     CMVNPDTGRFIHYYLAGAYDSNIALIVSYGNGRRENLERLADILRRMELRGSDLSTNR
                     DFHYGILNFCLGLHPMLKPDTKFVLPYLASVGALAKEIEATDPEAAYASLRKRYSQLY
                     GKPGDLAVSQKLTLVARPVEQLLSRPHLCMGWLVLNHRRSFKIENGKVVWLRNPMFVL
                     RDLYHYLRLETRENAPPSHVIWDHDLVLLNQACSFAAELEKAAGIDAEQAFQDSLARS
                     EENSFTKFIDLDRDLRAANGSSAFAQAINRRGAGLFEKACASYVGWTAGAELMSLVVQ
                     LGVRCGAFEFGVDAELYPITPEKFLSAETQKQLTRALAPPPKASADQIVAVTGGMFYA
                     QESPSTPPFLTVGHHFEKGDPIYIVEVMKMFNKIYAEFSGTVEEILVKGSGTVVRKGQ
                     PLFRVKPDEVIHLETDAERDDRRRKHTDEILKSV"
     gene            5478..6281
                     /locus_tag="PKK76_07685"
     CDS             5478..6281
                     /locus_tag="PKK76_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68390.1"
                     /translation="MLRGFTAMSMVFASCAVLAETPAGFHEDGIYLDLRGGQVWKRSG
                     SVMRDLENGQKDSFTGQEFGYTTFLRGNSDQIIANAAANSSAPDLSITSRNLGFGFEY
                     GLFNYLGIGFSILDERMNVRNIRSNISSDQTTLVALATAYPSASSAAYNQALINLEFL
                     DPTVRVNRLPYLHATTVNFQMRFHPLNGTLDPYAGFSLGIGRESQLPTNVLRPALHAG
                     LRVLTRSVYTGIELEMSFLEMGRKGTLPIPPHKQLTQTQFNIYAGLPLE"
     gene            complement(6305..>6843)
                     /locus_tag="PKK76_07690"
     CDS             complement(6305..>6843)
                     /locus_tag="PKK76_07690"
                     /inference="COORDINATES: protein motif:HMM:NF022447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HNL68391.1"
                     /translation="FGGSKFEQQKSLIEPLLVKESAPYSYVMVVDLHTGYGERGKLHF
                     FPNAIKDATIRKNIETVYAGYQVDYGDTADFYTTTGDFSEYVGKLLTAKKYIPMVFEY
                     GTLDSQTTSGSIKSIHNMIVENQGYWNGYESEGDLNEVKHRFREHFYPSSQVWRTKVI
                     AISAEVFPLVVDRFSKLQ"
BASE COUNT         1672 a         1830 c         1656 g         1685 t
ORIGIN      
        1 caatggtccc ggtcaaagct gttttacttc gacgaaaatt cgtaatgcag ctgtatgcgc
       61 gtcacgagtg ctggaatcgg ctcggacgaa tcagtgcaga ggcggccttg attgcgctgg
      121 aatctcgaga ggcactcacg ttcgagtctc tcgcggaacg ctatcgaaaa ttcctccagg
      181 actacgctgc atttccttat aagatcccca actaccaaat tgcctcctac cttggaatca
      241 cggacgtcgc cctttcgaga ttgagaaaac gcatgaatct gatctgatag aattaaccag
      301 ggttaaggac aaaccagttc ttacccgggt atacttgggc catgaacaaa acagtgacta
      361 ttcttatgat cgccgcgtct gttagctgcc tgacgctagt gatctattgc atttacggac
      421 atctgagaat tactcgggta tctcagggag tgccgattcc aaactatgaa acagaagcat
      481 ctgcgctcct gatcatcgac atgcagaggg atgccaccga gccatctggg cggctggtca
      541 tgaatccggc cgcgacagat cggatcatag aaaacctcaa tgccgttacg ccagccttgc
      601 gcaaacgccg cactctcttc gtgatgattc ggcatgagtg ggaggaaaac tggattcttc
      661 aaatacttac gaatcattcc ttacgaccag gaacgcgagg aagcgaagta gatccaagaa
      721 ttgcggtgaa gccggacaca atcatcacga aacaaaccag cgacagcttc ggaacaccgg
      781 cccttgacgc ctatcttcga gagaacaaga tttcgcatct ctacttgagt ggtaccggtg
      841 cgaatcattg cattcgccgc acggcagagg cagctctgca gcgcggttac aaagttacag
      901 tgctcagaga gctcgtcgcg ggcaaaacgg atgcagaaac gatagaaggg ctacaaagca
      961 tggaaaagtc aggtggaaaa atagcctctg tcgaatcgat tcgctgaaca ggatgccctg
     1021 gcaacgaaag cccgatcagc cgcgacgata ccgtgccagc tcgccgagca tgtgcacctg
     1081 actgatcaga atttttctga agtcgtccag atgcaggctt tcgttgtcag agtgcggatt
     1141 gctgtacgga tcctcaacgc ccagaagaac agcaggaatt ccgcctaact cctgcgaaag
     1201 cggttgcaca aacggaatgc ttgctccgca acccacgaaa cgagtctttt gcccgtaccc
     1261 tttctcaaga gaacgcgccg caatgtcaaa aatcggatcg tttaaatcct gaactgtcca
     1321 ccagtctgca gccgcttctg gagtgatggt aatttcaaaa ccacgcgggc aatgactgcg
     1381 gagaaaatca gagaggatgt cctgggtctt ttgaggcttc atgtccggga cgattcgcat
     1441 ggacacgcgc gcccaggctc catcctgaat gatgttggca accatcttgc gcgaaccact
     1501 ttcaaaagca agaacgctca gactcggctc tctccacatg cgctttgcca attcttcact
     1561 ggtgcctctc aacggtaaat gatccggaag ctgcgttcca gcgcgcacga tcttctccag
     1621 atccagcgta gagaactctt ttatttccgc aacagacaaa ggacgaacgt catccaggat
     1681 acccggaatc agaatgtttc cctcttcatc cgtcagcttt cctagaagtt gcgcaagagc
     1741 aatggcagga tcaggaacga cgccacccca gaaaccggag tgaaccggtt gcggcaatcc
     1801 gcgaagctcc acatccacgg aaaccagtcc acgcaaagaa gtcgtgatgc tcggaatgcc
     1861 agtgtcgatg ttgctgcaat cggttaagat catgcagtct gcacggagtt tctcacgatg
     1921 tctgcgcacg aactccgcaa gatgcgaact gccgatctct tcctctccct cgatcagaag
     1981 tttcacgttc accggcaatc gtccggcggt attgagatag gcagatatgg cagcagtatg
     2041 agcaagtatc ccgactttat catccgcagt gcctcgcccg aaaagtctcc cgcctctttc
     2101 cacaggatcg aatggtttag aattccaacc tttttcgtct cccggaggct gaacgtcatg
     2161 atgcgcgtac aacagcaccg ttggcaaatc tggatgttcc atccattccg caaacacata
     2221 cggatgcacg tcgccgaatt ggatcacttc cacgttcttc attccagaag aatggatgtg
     2281 ttctgcggcg gcgctggcac tgtctcttac ttgagatgga tcaaatccgt caaaactgac
     2341 gccaggaatg cgaaccagct ttttgagttc ttccagggag tggttcaatc tggaagtgca
     2401 atgatcgagg gctttttgtg tggcagcatc gggggctccc accttcagcg attcgccgtg
     2461 accctcccca tggtgatgag aatgatccgg cgaatgcgtg tgatcgtgct ctgcgatgac
     2521 tatctccgaa agacgttaga cgctcttgag tatctcatct gtatgcttgc gcctacgatc
     2581 gtcgcgctcc gcatctgttt cgagatgaat gacttcatcc ggtttcacac ggaaaagtgg
     2641 ctgtcctttt cgcacaaccg ttccagatcc tttcaccagg atttcttcca cagtccctga
     2701 gaattcagcg tagatcttat tgaacatctt catgacttca acaatgtaga tcgggtctcc
     2761 cttttcaaag tgatgaccga ccgtcaggaa aggaggcgtg ctgggcgatt cctgagcata
     2821 gaacataccg ccggttacag ctacaatttg atccgcggac gctttcggtg gtggcgcgag
     2881 agcacgagtc agttgctttt gcgtctctgc gctgaggaac ttctcaggag tgatcggata
     2941 aagctctgcg tccactccaa actcaaacgc gccacaacga acgcccagct gcacaaccag
     3001 actcatcaat tctgcgcctg cagtccaacc tacgtaggac gcacatgcct tttcaaagag
     3061 gccagcaccg cgacgattga ttgcctgcgc gaaagcagac gatccatttg ccgcgcgaag
     3121 gtcccggtcc agatcaatga acttcgtaaa tgaattctct tcggatcgcg cgagactatc
     3181 ctggaaagcc tgttctgcgt cgattcctgc agccttttcc agctctgccg cgaaagaaca
     3241 ggcctgattg agcagtacca ggtcgtgatc ccagattaca tgcgatggag gagcgttctc
     3301 acgagtttcg aggcgcaggt agtgatagag gtctctcaaa acgaacatgg gatttcgcaa
     3361 ccagacaacc tttccgtttt caattttgaa agatctgcga tgattgagca cgagccaacc
     3421 catgcaaaga tgcggacgag agagcaattg ctctacgggc cttgcaacca gagtcagctt
     3481 ctggctgacc gcaagatctc caggcttccc atacaattgc gaataacgtt tgcgaagaga
     3541 ggcgtacgcg gcctctggat cggttgcttc aatttctttt gcaagggcac caacggatgc
     3601 aaggtacgga agcacaaact tcgtatccgg cttgagcatt ggatgaagac ccagacagaa
     3661 attcaagatt ccatagtgaa aatcacggtt cgtagacaga tctgatccgc gcagctccat
     3721 gcggcgcaaa atgtccgcga gacgttccag gttttcgcga cgaccgttgc catagctcac
     3781 gatcaaagca atgttggaat cgtatgcacc tgcgaggtag taatggatga atcggccagt
     3841 gtcaggattc accatgcaga tgccctgatc gtctcgaatc tcgtttgcta caggtggtga
     3901 ccagaattca atgagaccgc ccgaagacgg ctgcaaagct tcgttctgtg cattgagacg
     3961 aatttcgccg cctgccacat tgcgcttgat tctctcaggc tttggaagac gtggaccatg
     4021 cgctgcgcaa agtgccattg cttctaccag agattctacc acaaagaagt cactcttgtt
     4081 tgccggattc tcgaaacgaa gtgaatagac catctccgtt acacgatgtt ctacttgaat
     4141 tcgagtattc atttccatga aatagaaatc atggcctgaa ataatacatt cgaacgtgga
     4201 agcagagttg aggcctgtgg cagtaccaaa cttctcgccc tgctcttcca tacgcgccag
     4261 aagtgcacga tcgacaccaa gggctttcgc atgatccgct ctacctctgg actccgcgat
     4321 ctgaatttca gccgcgtaca gttcatccgt caacgaaagt tccacgagct tttgttcatg
     4381 catctgaagc gaacagtctc tgccgccaag tgcaatggac cattgtccgt ttcctagaag
     4441 ctggatttcg ttgtgtcttg tcttttcaat gttgagttca atgaggaagt tcttgttatc
     4501 tgcatcaccg gttgcttttg attctgcgag cacttcttgc accgcgtttt ctacttcaga
     4561 aatcttagca acgatcctct gtccttttcc accgccgccg ccaatgtatt tgaggcgaaa
     4621 tcgttttcca ggattttcag agaacaattt gttgagttga acagacgctt ctgtttgcag
     4681 atcctttacg ctgagaagag gagctctctt cgcgtacgct gcctcgagaa tctgctctgc
     4741 cacttctgct ggaccttctg atcccgcgcg tttctgcgcg tcggcaaaat caattccatt
     4801 gtctttggcg attttctcaa gacccttttc tccgccggcc tttgtgagaa gagcgagcgc
     4861 tgaaatgttg tccagtccag gagtaacaga aaggccaagc ttacgcgcga gcttctttgc
     4921 catgtctttg gcaccggcct gcttgtgcac accggaagcc ggccccataa atccaatgcc
     4981 cgctttttcg atctcttcta cgaactctgc atcttctgcc atgaagccgt agcctgagaa
     5041 gatgtgagta taattgcctt tgactgcgaa gtcgatgatc tgctgaattc gaatcttctt
     5101 ttcttctgcg ttggccccgg cgtagtctgc gacccggtgg atgcggttct gatcattgag
     5161 atggcggagc tcaggagcga tggtgtgtgg atacgtgact gagtcttttt cagacagcag
     5221 aattccatag cctgcaccga gtgattcaaa cacgtccatt gcttccttac ggattggccc
     5281 gcggcaaaca atgagcacgt tgaggtgcga gcaggaaaag gatgccaccc aggcggagcc
     5341 ggatgcgcga tatcgatcaa tcggtttaga tggagataac atggtcaaaa tggtttcgaa
     5401 gagaatttta cgggaaaggg gggcgtcaat ctgttttatc cggttgcccc gctgcggcgt
     5461 gcggtagact cggccttatg ttgcgcggat tcactgccat gtccatggtt ttcgcctcat
     5521 gcgctgttct ggcggaaacg ccggcgggtt ttcacgaaga tggcatctac ctggacctgc
     5581 ggggcggaca ggtctggaaa cgatccggca gcgtaatgcg agacctggaa aatggccaga
     5641 aagactcttt cacgggccag gaatttggct ataccacttt cctgcgaggg aactctgacc
     5701 agatcatcgc aaacgcggct gctaactcaa gcgcacccga cctttccatt acttcccgca
     5761 atctcggatt cggattcgaa tacggactct tcaattatct tggaatcggt ttttcgattc
     5821 tagacgagcg aatgaatgtg cgcaacatca gaagcaatat ctcttctgac cagaccacac
     5881 ttgtagcact cgccactgct tatccttctg cgtcttcggc tgcatacaac caggcactca
     5941 tcaacctgga atttctagat ccaaccgtac gggtcaatcg gcttccttac cttcacgcca
     6001 ccactgtgaa ctttcagatg cgctttcatc cgctcaatgg aacgctcgat ccttacgcag
     6061 gattttcact cggaatcgga cgcgagtcgc agcttccaac caacgttctt cgccctgcat
     6121 tgcatgcggg actacgtgtt ctgacgcgat ctgtatatac aggtatagaa ctggagatgt
     6181 cctttcttga aatgggcaga aaaggaaccc ttcccattcc accccacaaa caactgacgc
     6241 agacccagtt caacatctat gccggcctac ccctggaata agacgttaga cggcattgta
     6301 gcagtcactg cagtttagaa aatcgatcca ccacaagtgg aaatacttct gcagagatag
     6361 cgattacctt tgtcctccac acctgagaag aaggataaaa atgctcccga aacctgtgct
     6421 tcacttcgtt tagatcccct tctgattcat atccattcca gtatccctga ttctcaacga
     6481 tcatgttgtg aatggattta atagatccag aggtggtctg actgtcgaga gttccgtatt
     6541 caaataccat aggaatgtat ttctttgcgg tgagaagctt gcccacgtac tctgagaaat
     6601 cccccgtcgt tgtataaaag tccgccgtgt ctccgtaatc cacctgatac cctgcataca
     6661 cagtttcaat gtttttgcga atggtcgcgt ctttgattgc attcgggaag aagtgcaact
     6721 tgcccctttc gccatagcct gtatgcaaat caacgaccat cacataggaa tacggggctg
     6781 attctttcac taacaacggc tcaatcaatg acttctgctg ttcaaacttg cttccaccaa
     6841 agt
//
LOCUS       DAORFF010000149         6840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5688_length_6840_cov_4.072218, whole genome shotgun sequence.
ACCESSION   DAORFF010000149 DAORFF010000000
VERSION     DAORFF010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5688_length_6840_cov_4.072218"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..978)
                     /locus_tag="PKK76_07695"
     CDS             complement(121..978)
                     /locus_tag="PKK76_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68392.1"
                     /translation="MKRISILLIAIAGFTTQLFAEDPAPTPAADNDSVLPIQEQNLGF
                     DPTRLQTMGTGSFEYRNLPSKAQDFTLRAGGDYRFSFLDDNRRWWGVSGSVPITMHQG
                     GKWGANKTGLGDLNLGVRRVFPGDIRQVGFVDVTWMLGGQPGLSGGHSLIAPGYGLSM
                     NIADRMQFAGTVTYVTDIGSKDWSKHEKIRVLHFKPMILYGLSDVLLLKGAFDVGYSL
                     TDGVETVPYFRKVNNKLQLTPSVTAGYLLGDDHRWMAFGTFQMPLDKFQIGYREQYSL
                     KVGANYFFD"
     gene            complement(989..1459)
                     /locus_tag="PKK76_07700"
     CDS             complement(989..1459)
                     /locus_tag="PKK76_07700"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNL68393.1"
                     /translation="MSKWSVFSVLIFAACLSGQSTQKTNEAYVQDLKSGDPALQREAC
                     KQLGLRRADNTVEAITALLTSSTDVDVQVSAAAALGEIAEVRSTSPLLKVLNTNPSPR
                     LQYASLAALGGIARINGTGKHKAAVIEAATRVKSGSTDSLLQDLADKIIQMYQK"
     gene            complement(1521..2174)
                     /locus_tag="PKK76_07705"
     CDS             complement(1521..2174)
                     /locus_tag="PKK76_07705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL68394.1"
                     /translation="MQVENNPADKHIARAKEKPLGWFEDLYRDAARNADLIPWADDEA
                     NPNLRSWAKTASGQEVLGSAKTALVVGCGLGDDAEFISRFVPEVTAFDISETAIAWAK
                     ERFANTKVKYMTANLFELATSCDFVFESYTIQALPLDMRSRAVARLPEFMNPGGRLLL
                     VARGRENEQPLADHPPWPLSRADLSPLDAVLQTETFEDYVEGTDPGIRRFRILYKKP"
     gene            2206..2712
                     /locus_tag="PKK76_07710"
     CDS             2206..2712
                     /locus_tag="PKK76_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNL68395.1"
                     /translation="MRNRICILFCSFTFLILQHCSSPQKSEPLPYEWMILTSTKDLPP
                     FAGAEILVRHTSSGSIGWVLNGERTTIHKLGLNGLGLPPDLVVRMGGPVETGKISFLY
                     RDPGTDAVHFSRYLPTLIEILKSEPANVVVFAGYSGWAEDQLASEIRRGLWVRSSKPR
                     RSIFVPET"
     gene            complement(2709..3311)
                     /locus_tag="PKK76_07715"
     CDS             complement(2709..3311)
                     /locus_tag="PKK76_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68396.1"
                     /translation="MKIETVKGKGMVVAGNDVDTDRILPARFMKSIVFSGLEEHAFED
                     ERKAFSRRGDLHPFDDPKNSDAVFLIAESNFGCGSSREHAVQSLARRGIKAIAAESFG
                     EIFLGNCRTVGVTAFTMSAADLAEARKLAKAGESFTIDLIQSRVSAGSQTFAMQMDEG
                     VRKQFTTGKWDTLAELLNAKDAAREKGMSLPNFGTEHGLF"
     gene            complement(3308..4702)
                     /locus_tag="PKK76_07720"
     CDS             complement(3308..4702)
                     /locus_tag="PKK76_07720"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNL68397.1"
                     /translation="MGKTLYDKVFDEHCVREVSPGQYQLYIALHLVHEATSAPAFEML
                     RERGLKLAAPERTFGTVDHVIPTNGEVRPYSDTTAEALETELEKNAMEFKFRYFSSGK
                     KENGVVHVIGPEKGLTWPASTIACGDSHTSTHGAFGAVAFGVGTSQVRDILATQTVIM
                     PRLRVKKVEITGRTPEGVTAKDVVLYVISKLGTRAGAGFAYEFAGQVIEKMTMEERMT
                     VCNMAVEAGARVGYINPDETTFAYLRSRAFAPDDFESAVTRWKTFASDKDAEYSEVVS
                     FDVSNLSPMVTWGTNPAQAVFVSDRTPESPDRDDADALAFMGFSANESVAGRRIDVAF
                     IGSCTNGRLSDLEAAADVVRKARTTKAAHVKAVVVPGSHNVYNEAVALGLDRVFTDAG
                     FEFRQPGCSMCLAMNSDKLEAGQVCASSSNRNFKGRQGSPTGRTLLMSPAMVAAAALR
                     GYVCDVREFTGGVK"
     gene            4795..5652
                     /locus_tag="PKK76_07725"
     CDS             4795..5652
                     /locus_tag="PKK76_07725"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNL68398.1"
                     /translation="MEFRQIQTFLEIHRQGSFVNAAARLALTQPALSRQISLLERELG
                     AKLFFRSAAGTRLTPEGEKLLGPANDLEHSLRNIQESLKSKESISGRYTISCGGTIAA
                     YVLPQAVRKIRQKHPDLLVRVVEGDAYDTLRSVSGGESELGILSGQPADSELESEFYF
                     RDEIVPVVGLSHPLARRRRMSAASLGDFECVSHHPASAIRQAVEKKWKALRLKPRIVM
                     ELRSMESVLRSVEAGVGVGFVSRLSVSKKMRVLPISELVSQRDFYFCFRRRSRGMDQF
                     IDLLRNTGS"
     gene            5660..6526
                     /locus_tag="PKK76_07730"
     CDS             5660..6526
                     /locus_tag="PKK76_07730"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNL68399.1"
                     /translation="MKKVYYIIFSVFCVWVVFAMIPPEKAHAMPVFDSPHFRIAHRCG
                     QPPEATLYSCGKTVEQGLADFIEMDVHLTKDLRLAVIHDEKLDRTTNGTGPVSGLTMD
                     EISRLDAGYKYTADGVTYPFRGKGINIHPLEDFFKAFPNQRFYVEIKTEDLRAVPIII
                     GLIRSHHMENKIVIASVYGPPLAMMEELAPEVARCASFRESLGWVILTKIGLGGVFSF
                     HSHALALPPAGGHWIITRRMIESAKRQNVKIHMWTINDPEAMASWKDAGVDAVMTDKI
                     GLLGDVLTRMQK"
     gene            6523..>6840
                     /locus_tag="PKK76_07735"
     CDS             6523..>6840
                     /locus_tag="PKK76_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNL68400.1"
                     /translation="MIEVKSLTRKYGDLTVLHDVSLKIERGEFVAVTGPSGSGKSTLM
                     GLLAGLDRPTGGQVFLDHEEITSLTEEDLSRVRGQKVGFVFQNFLLVQTLTALENVML
                     PAEI"
BASE COUNT         1578 a         1737 c         1713 g         1812 t
ORIGIN      
        1 cccgtcaatt ccggctacgt tcccggatat gacatttgtc atttgggtct ggaaggttta
       61 gaaagttgaa tgaattgccg cgctcttgaa aagaacgcgg ccgagaggct aacgattgag
      121 ttagtcgaag aagtagttgg cacccacttt tagagagtat tgttctctgt agccaatctg
      181 gaatttgtct agaggcatct ggaatgttcc gaacgccatc cagcgatgat catccccaag
      241 taaatagcct gcagtgacag acggtgtgag ctgaagctta ttgtttactt ttctaaagta
      301 tggaacagtt tccactccat ccgtgagaga gtaaccaacg tcaaaagctc ccttgagtaa
      361 gagtacgtca gacaggccgt agaggatcat tggcttgaaa tgaagaactc gaatcttttc
      421 atgcttggac cagtctttgg atccaatgtc tgtcacgtat gttacagtgc ccgcgaattg
      481 cattctgtct gcgatgttca tggaaaggcc gtaacctgga gcaatgagac tgtgtccgcc
      541 agatagacct ggttgcccgc caagcatcca cgtcacatcc acaaaaccaa cctggcgaat
      601 gtctccagga aatacgcgac gaactccgag gttgagatct cccagaccag ttttattcgc
      661 gccccatttt ccaccctggt gcattgtgat cggcactgag ccggacacac cccaccagcg
      721 gcgattgtca tccaggaagc tgaagcgata atctccacca gcgcgaagag tgaagtcctg
      781 cgctttgctt ggaagatttc tgtattcgaa agagcctgtt cccatagttt gcaggcgagt
      841 aggatcgaat cccagattct gttcctgaat tggaagaaca gaatcattgt ctgctgcagg
      901 tgtgggtgcc ggatcttctg cgaagagctg agttgtaaaa ccagcgattg caataaggag
      961 gattgaaatt cttttcacgc gatgacctct acttttgata catctggatg atcttgtctg
     1021 caagatcctg gagaagagaa tccgtagatc ctgacttcac cctggtggca gcttcaatga
     1081 ctgctgcttt atgttttcca gtaccattga tacgtgcgat cccgccaagc gcggccagag
     1141 aagcatactg cagacgcggc gacggattgg tgttcaacac tttcagaaga gggctggttg
     1201 aacgaacttc tgcaatttct ccaagtgcgg cggcagcact cacctgcacg tccacatctg
     1261 tggaagatgt aagaagggcg gtgatcgctt ctactgtgtt gtcagctcga cgcagtccaa
     1321 gttgcttgca cgcttctcgc tgcaatgcag gatctcccga tttgagatcc tgtacgtaag
     1381 cctcatttgt cttctgggta gactgaccag aaaggcaggc tgcaaaaata agaacggaaa
     1441 atacgctcca cttgctcata ctgccagagg aaagctttgt acttactggt caatgcttat
     1501 tgggccccgg agggcccgga tcagggcttc ttatagagaa ttcggaagcg ccgaatgccc
     1561 ggatctgtgc cttccacgta gtcctcgaat gtctccgttt gaagcaccgc gtcgagaggg
     1621 gagagatccg ccctggacag aggccagggt gggtgatcgg ccaggggctg ttcgttttct
     1681 ctacctcttg cgaccaacag gagccttccc ccgggattca tgaactctgg gagcctggcc
     1741 actgctcgtg atctcatgtc cagtgggaga gcctggatcg tgtaggactc aaacacaaag
     1801 tcgcatgacg tggccagctc gaacaaattg gcagtcatat actttacttt tgtattcgcg
     1861 aaccgttctt ttgcccaggc gatggcagtt tcagaaatgt caaatgcggt gacttccgga
     1921 acaaatcgag agataaattc tgcatcatct cccagaccgc atccaaccac aagagctgtc
     1981 ttcgccgatc ccagtacttc ctggccgctt gcagtctttg cccacgatct tagattcgga
     2041 tttgcttcat catctgccca tgggatgaga tcagcgtttc ttgccgcgtc gcgatagaga
     2101 tcttcgaacc atccgagagg tttctctttc gcacgtgcga tgtgtttatc tgctggatta
     2161 ttttcaactt gcatactatc ctcactttat tgtagattct caacaatgcg aaatcgcatc
     2221 tgcattcttt tttgttcttt cactttcttg attcttcagc actgctcctc tccgcagaag
     2281 tctgaacctc ttccatatga atggatgatt ctcacttcca caaaagatct tccgccgttc
     2341 gctggcgctg agattctggt tcgtcatact tcctctggtt cgatcggttg ggttctcaat
     2401 ggagagagaa ccacaattca caaacttggt ctgaacggcc tgggccttcc accagatctt
     2461 gtcgttcgca tgggtggccc ggtggaaaca ggaaagatca gctttctgta tcgtgatcct
     2521 ggaacggatg ctgttcattt ttcaagatac ctcccgactt tgattgaaat ccttaagagc
     2581 gaacccgcaa acgtagttgt ctttgctgga tattccggct gggcggaaga ccagcttgca
     2641 tctgaaattc gccggggcct ctgggttcgt tcttccaaac cccggcgaag catttttgtt
     2701 ccagagactt agaagagtcc atgttctgtc ccaaaatttg gaagactcat tcctttttcg
     2761 cgcgcagcgt ctttcgcatt caacaattct gcaagcgtgt cccattttcc ggtggtgaac
     2821 tgcttgcgaa caccttcgtc catctgcatt gcaaaagtct gtgaacctgc acttactctg
     2881 gattgaatca gatcgatcgt gaatgactct cctgctttcg caagcttgcg tgcttctgcg
     2941 agatctgcgg cactcatcgt gaaggccgtc actcccacag tcctgcaatt tccaagaaag
     3001 atctctccaa acgattccgc ggcgatcgcc ttgatgcctc ttcgcgccag agattgcact
     3061 gcatgttcgc gcgaagaccc gcatccaaaa ttgctttctg cgatcaagaa taccgcgtct
     3121 gaattttttg gatcgtcgaa ggggtgcaga tcacctctgc gcgaaaatgc tttgcgttca
     3181 tcttcaaaag catgttcctc gagcccgcta aacacgatgg acttcatgaa tcttgccgga
     3241 agaatgcgat ccgtgtccac gtcatttcca gcaactacca ttccttttcc ttttactgtt
     3301 tcgattttca tttcacacct ccagtgaatt cacgcacgtc acatacatat ccacggagcg
     3361 ccgctgccgc taccatggct ggcgacatca agagagttct tcctgtggga gagccctgtc
     3421 ttcctttgaa gttgcggttg gaagagctgg cgcacacctg tcctgcttcc aatttgtctg
     3481 aattcatggc caggcacatg gaacatcctg gttgtctgaa ttcaaatcca gcatccgtga
     3541 acacgcggtc gaggccgagc gccaccgctt cgttgtagac gttatgcgac cctggcacaa
     3601 ccactgcttt tacatgcgct gcctttgtag ttcgggcttt tcgcactacg tctgccgcgg
     3661 cctcaagatc actcaatcga ccgttcgtgc aagagccaat gaacgccaca tcaatgcgtc
     3721 tgcctgccac tgattcgttt gcagaaaaac ccatgaatgc aagtgcatct gcgtcgtctc
     3781 ggtccggcga ttctggagtt ctgtcagaaa caaaaactgc ctgagccggg tttgtgcccc
     3841 acgttaccat gggagagagg ttggatacgt cgaaagaaac cacctcagaa tattctgcgt
     3901 ctttatcaga ggcgaacgtc ttccagcgcg tgaccgcact ttcaaaatcg tctggcgcaa
     3961 atgcgcggct gcgaagatag gcgaatgtag tttcatccgg gttgatatag ccaactctcg
     4021 cgcccgcttc cactgccata ttacataccg tcatgcgctc ttccattgtc attttctcaa
     4081 tgacttgtcc tgcaaattcg tacgcgaatc ctgcacccgc tcttgtgcca agctttgaaa
     4141 tcacatacag aaccacatct ttcgcggtga cgccttcggg tgttctgccc gtgatttcaa
     4201 ctttcttcac gcgaagacgc ggcatgatta cagtctgcgt ggcgagtatg tcgcgcacct
     4261 gggaagttcc cacaccgaat gcaaccgcgc cgaacgctcc gtgagtggat gtgtgcgaat
     4321 cgccgcaggc aatggtgctt gcaggccagg tgagtccctt ctccggacca attacatgaa
     4381 cgactccgtt ttcttttttc cccgaggaga aatatcggaa tttgaactcc atcgcattct
     4441 tttcaagttc cgtttccaat gcctcagctg tcgtgtctga gtatggtcga acttctccat
     4501 ttgttggaat cacgtgatct acagtgccaa atgttctttc gggtgccgcc agtttcaatc
     4561 cgcgttctct gagcatctcg aatgcgggtg cgctggttgc ttcgtgcacc aggtgaagag
     4621 cgatgtagag ctggtattgc cccggagata cttcgcggac gcagtgttcg tcgaatacct
     4681 tatcgtacag tgtctttccc atgttgggcc tccgtttgat cttattatac gccatctcta
     4741 gtatatctca aaataataag tagacatggg tagtataagg aaaacttata tagcatggaa
     4801 ttccggcaga ttcagacttt ccttgagatc cacaggcagg gttcttttgt aaacgcagcc
     4861 gctcggctgg ctttgactca gccagcgctg tccaggcaaa tctccctgct ggagagagag
     4921 ctgggtgcga agctcttctt tcgcagcgcg gccggtactc gcctgactcc ggagggagaa
     4981 aagcttctgg gacctgccaa cgatctagag cattctctgc gcaacattca agagagtctc
     5041 aagagtaagg aatctatatc tggacgttat acgatctcgt gcggaggtac gatcgcagcc
     5101 tatgtgcttc cacaggcagt gcgaaagatt cgccagaaac atccagatct tctagtgaga
     5161 gtggtggagg gagatgcgta cgacaccttg cgttccgtca gcggaggaga gtcagagctc
     5221 gggattctct ccggtcagcc agcggatagc gagctagaat ccgagttcta ctttcgagac
     5281 gaaattgttc cggtggttgg tctttcgcat cctctggcca gaagaagaag gatgtccgct
     5341 gcttctctag gagattttga atgcgtatcg catcatcctg ctagcgcgat tcgccaggcg
     5401 gtggaaaaga agtggaaagc ccttcgtttg aagccgcgca tcgtaatgga gcttcgcagc
     5461 atggagtcag tattgcgcag tgtggaggca ggagtgggcg taggcttcgt gtccaggctc
     5521 tccgtttcaa aaaaaatgag ggttcttccg atttctgagc tggttagtca gagagatttt
     5581 tatttttgtt tccgtcgacg gtctcgcgga atggatcagt tcattgattt gcttcgcaac
     5641 accgggagct gagggtttct tgaaaaaggt atactatata atattctctg ttttctgcgt
     5701 ctgggtggtt tttgcaatga ttccgccaga aaaggcgcat gcgatgcctg ttttcgactc
     5761 tcctcatttc agaatcgccc atagatgcgg tcagccgcct gaagcaacgc tctattcctg
     5821 cggcaaaacg gtggagcagg gtctcgctga tttcattgag atggatgttc accttacaaa
     5881 agatcttcgc ctggcagtca tccatgatga aaaattagat agaaccacga acggaacggg
     5941 ccctgtgtcg ggtctaacca tggatgagat ctcgcgattg gacgcaggct acaaatacac
     6001 agcagacggt gtcacctatc catttcgcgg caaggggatc aacattcatc ccctcgagga
     6061 tttcttcaaa gcattcccga atcaaagatt ctacgtggaa atcaagacag aagaccttcg
     6121 cgcagttccc atcatcattg gattgattcg ctcgcatcat atggaaaata aaatcgtaat
     6181 tgcatctgtt tacggccctc ctctcgcgat gatggaagag cttgctcctg aagttgccag
     6241 atgcgccagt tttcgcgaat cgcttggctg ggtaattctg accaagattg gtttgggtgg
     6301 agtcttctcc tttcattctc acgcgcttgc tcttccgcct gcgggagggc actggatcat
     6361 tacgcgtcgc atgattgaat ctgcgaagag acaaaacgta aaaatacata tgtggaccat
     6421 caatgatccc gaagccatgg catcctggaa ggatgccggt gtggatgcgg tgatgacgga
     6481 taaaattggc ctgctcggcg acgttcttac aaggatgcag aaatgattga agtaaagtct
     6541 ctaacgcgca agtacggtga cctgaccgta ctgcacgacg tgtctctgaa aatagagaga
     6601 ggggaattcg tagccgtaac cggcccttcc ggaagcggga aatctacatt gatgggattg
     6661 cttgcgggac tggacagacc caccggcgga caggtgtttc tggatcatga ggagatcacg
     6721 tctcttacag aggaagatct ttcgcgcgtg cgaggtcaga aggttggatt tgtatttcag
     6781 aattttcttc tggtgcaaac gttaaccgct cttgaaaacg taatgcttcc tgctgaaatc
//
LOCUS       DAORFF010000150         6830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5704_length_6830_cov_4.003985, whole genome shotgun sequence.
ACCESSION   DAORFF010000150 DAORFF010000000
VERSION     DAORFF010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6830
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5704_length_6830_cov_4.003985"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..758)
                     /locus_tag="PKK76_07740"
     CDS             complement(240..758)
                     /locus_tag="PKK76_07740"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNL68401.1"
                     /translation="MPRYVAFLRGVSPMNASMAELRTRIESAGFTDVKTVLASGNVVF
                     NSRSASNESLQKKIEAAIAAGPGRPFSTIIRSVESLESFLEEDPFEQFKLPAKAKKVI
                     TFLREANPNPPALPIKRDDVFILTMSKSEVFSAYVPDPKKGPIFMQMLEKNFGKDITT
                     RTLDTVKKCVSA"
     gene            complement(801..1403)
                     /locus_tag="PKK76_07745"
     CDS             complement(801..1403)
                     /locus_tag="PKK76_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68402.1"
                     /translation="MSALQNTMSWSARNFIFLLAIALALLVTCSPPQAICYIVNKYDK
                     PVTAKVTFPSRHIFEIFRDAEFTLTDAKHEKYTGDRARSSADENKLILEITVPPHSAV
                     AISNLGGHSYALNRHCSEILEKAPNSEEGDRSSYEHGCESLIYRAASLQVDTPDGTIQ
                     LRGLQIAMAFRRQEALAYVLELDPDLPEFTPKEPRLCCCL"
     gene            complement(1405..2232)
                     /locus_tag="PKK76_07750"
     CDS             complement(1405..2232)
                     /locus_tag="PKK76_07750"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNL68403.1"
                     /translation="MKRISILLLVGTTAIALLFSSATAQNKQWKPGSRAPQAGDEYVN
                     PKDGTVLVFIPGGEFTMGSDSGDADEKPAHKQTVQGFWLGKYEVTNQQYQKFRDAEGY
                     PDPQAMDDPAFNAPNQPVIGVKFMHAQSYVKWAGLRMPTEAEWEYAASGGKNLKYPTK
                     TGEINHDLANYLGKEGADKWEDVPSPVGSFPPNPFGIYDLAGNAWEWTSSMYRNYPYS
                     ATDGRENQTDRRHRVMRGGTFHFGEKYIRTTHRHHFAMHLRLDYAGMRMAASTDQLK"
     gene            complement(2229..3329)
                     /locus_tag="PKK76_07755"
     CDS             complement(2229..3329)
                     /locus_tag="PKK76_07755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL68404.1"
                     /translation="MKKLTLALIFLIVPLFADEVPDQDLDVPYEPSHPKVVEAMIKAA
                     AVTGQDTLFDLGCGDGRIVIAAAKLGARAIGYDIDEQRLMEARERAHVAGVESKVEFR
                     REDIFKADLSKATVVTMYLLDEINLQMRPRLFRMLKPGTRVVTHAFHMSDWKPDSFIE
                     NERARDHKIMYWVIPASAGGTWTWKAGGQDYRMDLIQKFQVVTGTIRSGNSSANIETK
                     INGKSFTVDATLRGGKETKVSIKGMIDGDRMTGNVETAGVRSALDAKRTLTNPEGKWR
                     IEMAETKKVFSGNLTLVKDAAGWTAEFEADKRYAITDLYVWGDSVYFRVPILGAENPA
                     IYTGRLQNEGTGFLTNDAASFTTSFTVKKAAK"
     gene            3415..4692
                     /locus_tag="PKK76_07760"
     CDS             3415..4692
                     /locus_tag="PKK76_07760"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNL68405.1"
                     /translation="MGQASQEFVAEAARAGRAARHAFRILRSSSSAMRNHALLILAES
                     LESDSVQQNVFAENQKDLAAALANDLKASLVDRLKLDVKRMKEMASAVREIAQFPDPV
                     GEVIRGRTLKSGVRMIQKRVPLGVVFTVFESRPNVTVDIGALCVKSGNAAILRGGKEA
                     IHTNIALHSIFQDALVKAGLPAESIQIVTETDRELMLAFLHQDQLIDLVVPRGGEALI
                     QFTVENSRIPVVKHDRGVCNLFVDQNADFDLVLRVVANAKLQRPSVCNAIENLLVHRA
                     YPHTKKLLMGLEQEGAILLGDQESRDTYEGVGEIKNPDAEYAQEYLDNRLAVKMVGSV
                     EEAVEFIYKYGSGHSEAILSRDVEAIDRFVASVDSAAVFVNCSTRFHDGGQMGMGAEV
                     GISTGRLHVRGPMGVEHLTTTTYVMEGEGQVRL"
     gene            complement(4689..5270)
                     /locus_tag="PKK76_07765"
     CDS             complement(4689..5270)
                     /locus_tag="PKK76_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNL68406.1"
                     /translation="MARPREFDSDDALQASVNLFWEKGYHATSITDLTEAMGVLRGSL
                     YKAFGDKHTLFIKSLKAYSENLLEGMRKNLETSKPVEALNRTLDIVVGTAHGKAARKG
                     CMIANSALELVPEDPVTAVCVNKHYDAMESLLTRALEKARTDGKLKTEMSPTEAAQAI
                     LVWIQGTHTIGKTKTGKVRAANSLAQLKKTLNI"
     gene            complement(5353..6303)
                     /gene="sigJ"
                     /locus_tag="PKK76_07770"
     CDS             complement(5353..6303)
                     /gene="sigJ"
                     /locus_tag="PKK76_07770"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HNL68407.1"
                     /translation="MTHTSLYTEHRRLLFSIAYRMTGSSSDAQDILQEGYLKFQNVAL
                     NAVNNSRAFLITLITRMCIDFLDSAYERRRTYIGPWLPEPITESPEEDYVRMESLSTA
                     FLLMLERLSPVERAVFLLRDVFDVDYAEIASMLEKSEENCRQISSRARKHVSSEAIRF
                     ETPPQKKDQLFRAFLRAVQKGDVHSLGKLLCEDAKMISDGGGKVNAARNVVSGAAKCI
                     RGILGVAKKQPPDAWPVLVEGGGEPALVLLTGGKVYTILNLSYRNGQIDAVYSILNPD
                     KLQNVKVPPLARVASIVFSYGLIPFRRIFGLVARALSRAK"
     gene            complement(6300..>6830)
                     /locus_tag="PKK76_07775"
     CDS             complement(6300..>6830)
                     /locus_tag="PKK76_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68408.1"
                     /translation="IESRTAILAADPESENAWLGTKIGSGKKLRAACLDVALDRLPEP
                     DHIFAQDIAEPLYLSVHSASANLGPGQVIHVARYLKEGEEAGEGTKKQLRDFLARFQP
                     GSEDRIVQERFLPDMIVAHDFQDTTEQPGVEIDSAKPLFRAGDWVRGSGMLSDRSAFT
                     AVTAAEKAMARIRAKS"
BASE COUNT         1479 a         1678 c         1806 g         1867 t
ORIGIN      
        1 cgggcttgca cacttccatc atcggagggc cttctgacag tccattctcc aggctctatg
       61 cgaacgatgt ggacaactgg gtgcgggggc ttttgaaaaa agtggggttc tgaaaacaga
      121 gggcgtggga attgccgggc cacgaattga cgttaggcgg tgttggatga agaaatttcg
      181 cactgcgaaa tttctcctat gggaagtgcg ctgtattttg accgcatgcg aatcgcccgc
      241 tatgcggaaa cgcatttctt cactgtgtcc agagtgcgag ttgtgatgtc ctttccaaag
      301 ttcttttcca gcatttgcat gaagatcggc cctttttttg ggtcaggcac ataggcagaa
      361 aacacttcgc tttttgacat ggtgagtata aaaacgtcat cccgttttat ggggagcgct
      421 ggaggatttg gatttgcttc gcgcaaaaag gtgatcacct tttttgcttt cgcgggtagt
      481 ttgaactgtt cgaaaggatc ctcttcaagg aatgattcca ggctttccac agaacgaatg
      541 attgtgctga agggtctgcc cgggccggcc gcgatggcgg cttcgatttt tttttgcagg
      601 ctttcgttgg atgcgcttct tgagttgaaa actacgttgc cacttgccag cacagtcttc
      661 acgtccgtga agcctgcgga ctctatgcgc gttcttaact cagccatact ggcgttcatc
      721 gggcttactc cgcgaagaaa tgcaacgtag cggggcataa gtcactccag gggaaaagaa
      781 tgtattcgaa acgattgctt ctagaggcag caacacagac gaggctcttt tggtgtgaat
      841 tcaggtaaat ccggatcgag ctcgagtacg taagcgagag cttcctgccg gcgaaatgcc
      901 atggcgatct gcagtccccg gagctggatc gttccgtctg gagtgtccac ttgcaaggaa
      961 gctgcccggt agatcagaga ttcacagccg tgttcgtagg acgatcgatc tccttcttca
     1021 gagtttggtg ctttttccag tatctcagaa caatgtcgat tcagtgcata cgaatgcccg
     1081 cccagattgg agatggcaac cgcagagtga gggggcaccg tgatttccag aatgagtttg
     1141 ttttcatccg cagatgacct ggcgcgatcg cccgtgtact tttcgtgctt cgcgtctgtc
     1201 agtgtgaatt cagcgtctct gaaaatctca aatatgtgac ggctcgggaa agttactttt
     1261 gcagtcacag gtttgtcgta tttgtttacg atatagcaga ttgcctgagg tggagagcat
     1321 gtaacaagca acgcaagggc tatcgccagc aggaaaatga aatttcgcgc gctccacgac
     1381 attgtattct ggagcgcgct cattctattt caattgatct gtagatgcgg ccattcgcat
     1441 tccagcataa tccagcctga ggtgcatcgc gaaatgatgt cgatgcgtag tgcgaatgta
     1501 tttttcgcca aaatgaaacg ttccgccccg catcactctg tgtctgcggt ccgtctggtt
     1561 ttcgcggcca tctgttgcac tgtacggata gttgcgatac atgctcgaag tccactccca
     1621 ggcattcccc gcaagatcat agattccaaa cggattggga ggaaaacttc caacaggtga
     1681 cggaacatct tcccatttgt ccgcaccttc ttttcctaga tagttcgcaa ggtcgtgatt
     1741 gatttcgcct gtcttcgtgg gatattttag attttttccg ccggaagctg catattccca
     1801 ttcagcctcc gttggcatgc gaaggccagc ccactttaca taggactgag cgtgcatgaa
     1861 cttcactccg atcacaggtt gattgggggc attgaacgca ggatcgtcca ttgcctgtgg
     1921 atcggggtag ccttccgcat cgcggaattt ttgatactgt tgattggtga cttcatactt
     1981 acccagccag aatccttgca ctgtctgctt gtgcgcgggt ttttcatccg cgtcgcctga
     2041 atcggatccc attgtaaatt caccgcctgg tatgaagacc agaacagttc cgtcttttgg
     2101 atttacgtat tcgtctccgg cctgaggtgc tcgacttcct ggtttccatt gtttgttctg
     2161 ggcggttgcg ctcgaaaata aaagggcgat agcagttgtg ccgactaaaa gcagtattga
     2221 gattcgcttc atttcgcggc cttctttaca gtgaagctgg tagtaaagga agcagcatca
     2281 tttgtgagaa atcccgttcc ttcgttctga aggcgtcctg tgtagatggc aggattttcc
     2341 gcgcccagga taggtacgcg aaagtaaacg ctgtcccccc agacgtagag atctgtgatc
     2401 gcataacgtt tgtccgcttc gaactctgca gtccatccgg ctgcgtcttt caccagagtg
     2461 aggtttccgc tgaacacttt ttttgtttcc gccatttcta ttcgccactt tccttccggg
     2521 tttgttagcg tccttttggc gtcgagcgcg cttcgcacgc ctgcagtctc gacgttgccg
     2581 gtcatccggt ctccatcaat catgcctttg atagatactt tagtttcttt accacctcgc
     2641 agtgtcgcgt cgactgtgaa agattttccg ttgatttttg tttcaatgtt tgcggatgaa
     2701 tttccgctgc gaatcgtgcc tgtaactacc tggaacttct ggatgagatc catacggtaa
     2761 tcctggccgc ctgctttcca ggtccaggtg cctcctgccg acgcagggat cacccagtac
     2821 atgattttgt gatcacgcgc tctttcgttt tcaataaatg agtccggctt ccagtcagac
     2881 atatgaaatg catgagtgac aactctggtt cccggtttga gcatgcgaaa tagacgcggc
     2941 ctcatctgca gattgatttc atcgagcaga tacatggtta caaccgttgc ctttgaaaga
     3001 tctgctttga aaatatcttc gcgcctgaac tcaactttgc tttctactcc ggccacatgt
     3061 gctctttcgc gcgcctccat gagacgctgt tcgtctatgt cataaccaat ggccctggct
     3121 cccagctttg cggctgcaat tacgattctg ccgtctccgc aacccagatc aaatagagta
     3181 tcctggccgg tgactgcggc cgccttgatc atggcttcta ctaccttcgg gtgggaaggt
     3241 tcgtatggaa cgtcgagatc ctgatcgggc acttcatctg caaaaagagg cacgatcagg
     3301 aagatcaggg ccagagtgag ttttttcata tgttttgcag attttggacg caggcggcat
     3361 tgatcaaggc tgaatttttc tttgcactgc cggaacaaaa tccatcccgt ccatgtgggc
     3421 caggcaagtc aggaatttgt tgcagaagca gccagagcgg gtcgagcggc caggcatgcg
     3481 tttcgcattt tgagatcctc ctcttctgcc atgaggaatc atgctctttt gatccttgct
     3541 gaatcacttg aatcggattc tgttcaacaa aacgtattcg cagaaaacca gaaggatctc
     3601 gcagccgctc tcgcaaatga tctcaaagct tctctcgtgg atcgtctgaa gctagacgtg
     3661 aagagaatga aagagatggc ttctgccgtt cgcgaaattg cgcaatttcc agatcctgtg
     3721 ggtgaagtca ttcgcggtcg cactctgaag agtggggtgc gcatgatcca gaagcgcgtt
     3781 ccgctcggag tagtcttcac agtctttgaa tcgcgtccca atgtgactgt ggacattggc
     3841 gcactttgtg tgaaaagcgg aaacgcggct attcttcgcg gaggcaagga agccattcac
     3901 acgaacattg cacttcattc tatttttcag gatgctctgg taaaagcggg cctgcctgcg
     3961 gaaagcattc agattgttac ggaaacggat cgcgaactca tgcttgcgtt tttgcaccag
     4021 gatcagctga tcgatctggt tgtgccgcgt ggcggggaag ctctgattca atttaccgtt
     4081 gagaattcgc gcattccagt tgtgaagcac gaccgcggtg tgtgcaatct cttcgtagat
     4141 cagaatgcag atttcgatct tgttctgcgc gtggttgcaa atgcgaaatt gcaaaggcct
     4201 tctgtgtgca atgcgattga gaatctgctg gtccatcgcg cgtatccgca tacaaagaaa
     4261 ctactcatgg gccttgagca agaaggagcg attttgcttg gcgaccagga gtcgcgcgat
     4321 acttacgagg gcgtggggga aatcaaaaat ccagacgcgg agtatgccca ggaatatctg
     4381 gataacaggc tggctgtgaa gatggttggg tctgtggaag aagcagttga gtttatttat
     4441 aaatatggca gcggacattc agaagcaatt ctgtccagag acgtggaagc aatcgatcga
     4501 tttgtagcaa gcgtggactc tgcggccgtg tttgtgaatt gctccacccg ctttcacgat
     4561 ggtggccaga tggggatggg cgcagaagtt ggaatttcca ccggaaggtt gcatgtgcga
     4621 ggcccgatgg gagtggagca tttgactaca accacctatg tgatggaagg cgaagggcag
     4681 gttcgactct agatgtttag agtctttttg agttgagcca gagagtttgc agctcttact
     4741 ttccctgttt ttgtttttcc aatcgtgtgt gtgccttgaa tccagactag aatcgcctgt
     4801 gccgcttccg tagggctcat ttcagttttg agttttccgt ctgtcctggc cttttcaagg
     4861 gcccgcgtga gcaggctttc cattgcatcg tagtgcttgt tgacgcacac cgcagtgaca
     4921 ggatcttccg ggaccaattc taacgcgcta tttgcgatca tgcaaccctt tctggctgct
     4981 tttccgtgag cggtgccgac tacaatgtcc agggttcggt tcaacgcttc cactggtttg
     5041 ctggtttcca ggttctttcg cattccttcc aggaggtttt ctgaatatgc cttcaaggat
     5101 ttgatgaaga gagtgtgttt gtctccaaac gctttgtaga gactgcccct caggactccc
     5161 attgcttccg ttagatcggt gatggatgtt gcatggtagc ctttttccca gaacaaattc
     5221 acggaagcct gcagggcatc gtcactgtcg aattcgcgcg gtcgggccat gtggtgagta
     5281 cgcgaaaatg acgtttcgca gtccataaat tttttataac ttcgcacgac tttcgcgtac
     5341 tcttcgcatg ctctatttcg cgcgcgaaag tgctctcgcg acgagtccga aaattctgcg
     5401 aaatgggatt agcccatacg aaaacacgat cgacgcgacg cgagccagtg gtggcacttt
     5461 cacgttctgg agtttgtccg gattcaggat gctgtagaca gcgtcgatct gcccgttgcg
     5521 gtacgagaga ttgaggatcg tgtagacttt gccgcctgtg agcaaaacga gtgcaggctc
     5581 tcctcctcct tctacgagca caggccaggc gtcaggcggt tgtttttttg caacaccgag
     5641 aatgccgcga atgcatttgg cggcaccact gaccacgttt cgcgccgcgt tcaccttacc
     5701 gccgccatct gaaatcattt tcgcgtcttc gcacagcagc ttacccagag aatggacatc
     5761 tcctttctga acggcgcgaa ggaacgcgcg aaaaagctga tcttttttct gaggaggagt
     5821 ttcaaaccga atggcttccg aggaaacgtg tttgcgcgca cgcgaagaga tttgtctgca
     5881 attttcttct gatttctcga gcatcgaggc gatttctgcg tagtccacat cgaatacgtc
     5941 tcgaagtaga aacacagcgc gttccacagg actgagcctt tcgagcataa gcaagaaggc
     6001 cgtggataga ctctccattc gcacgtaatc ttcttcggga ctttccgtga ttggttcggg
     6061 aagccagggc ccaatgtatg tgcgacgcct ttcataggcg gaatccagaa aatcgatgca
     6121 catcctggtg atgagagtga tgaggaatgc ccgcgagtta tttactgcat tgagcgctac
     6181 gttctgaaat ttcagatagc cttcctggag aatatcctgc gcatcagacg agctccccgt
     6241 catcctgtaa gcgatggaga aaagaagccg tctgtgttcg gtgtatagac ttgtgtgggt
     6301 catgactttg cgcgaatgcg agccattgct ttttctgcgg ccgttactgc agtgaaagca
     6361 cttcgatcgg agagcatgcc tgatccgcgc acccagtccc cggcgcgaaa cagtggtttg
     6421 gcggagtcaa tttctacccc gggctgttcg gttgtatcct ggaaatcgtg tgccacaatc
     6481 atgtccggta aaaatcgttc ctgtacgatt cgatcttcgc tgcctggctg gaacctggca
     6541 agaaaatcgc gtaactgctt cttcgtccct tcgcccgctt cttctccttc tttgagatag
     6601 cgcgctacat ggatgacctg ccccgggcca agattggcgc tggcggaatg gaccgataga
     6661 taaagaggtt cagcaatatc ctgtgcgaag atatgatccg gctccgggag gcggtcaagt
     6721 gccacgtcga gacacgcagc gcgaagtttc ttgccacttc caatctttgt tccaagccag
     6781 gcgttctccg attcaggatc ggcggccagg atagcggtcc tgctttctat
//
LOCUS       DAORFF010000151         6787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5770_length_6787_cov_4.030600, whole genome shotgun sequence.
ACCESSION   DAORFF010000151 DAORFF010000000
VERSION     DAORFF010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5770_length_6787_cov_4.030600"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PKK76_07780"
     CDS             <1..428
                     /locus_tag="PKK76_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HNL68409.1"
                     /translation="LMGENGPIKVAKFNFLVKENGEVWINQAVGNEPGAGANETSNQW
                     QNPVLLDKIKVRTVEFPRELKKEGNGFYEDTPESGPMVVPQERNDPTLLQGFVEASNV
                     NLVSQMVQMIEVQRQYEANQKSVTTHDQLLGRLINEVAR"
     gene            489..914
                     /locus_tag="PKK76_07785"
     CDS             489..914
                     /locus_tag="PKK76_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68410.1"
                     /translation="MKKSFLVCAVWVLALSLEGGCIQSPSPSANPDAVILLLFGSVAT
                     VRPVQATCNRTNFQCFEYNTGFTGSQMTADCASMFLGVLSASGCPTSSRLGSCYITNR
                     GYATNAFGTTAALRFETPAWAFGTAQGTCSAYGGTFAQN"
     gene            complement(949..1584)
                     /locus_tag="PKK76_07790"
     CDS             complement(949..1584)
                     /locus_tag="PKK76_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNL68411.1"
                     /translation="MAIRILILEDDPNITRTLAEMFASNSDFQLLGAYSTGADFFRDV
                     HKAADVLLLDFELPDQNALSVLRRLKSSEALRDMKVLIFTAFEDETRLIHAMEAGANG
                     FLLKDTSPELLLAEIRSVHLGGAPITARMARALLGHFAPPSTRADGLLTQQERRVLQL
                     LSMGLTYAEAAHKLGISINTLKNYTENTYRKLEVRSKSGAIEKGRARGLIS"
     gene            complement(1593..3488)
                     /locus_tag="PKK76_07795"
     CDS             complement(1593..3488)
                     /locus_tag="PKK76_07795"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL68412.1"
                     /translation="MSPASCETSDRITAKNTLAYGEGHLNGTSRAFIARYSILLCFLC
                     SGIEAANPIRLNSLVGRTYIGERLEEFEDTSGSLAFADVQSQTFHNVGSKTVHHGFTD
                     SVWWFRFSYDCAGVPEDQVYFLSMFNFRMYDASIYVATDGSLVRQMQSGMIGKRDVRT
                     TDIAFRLPRTKNAATVYLRVKGITPVRFAPSIQSEEELRNNQLTSDMLIGLAMGGQFS
                     LAIFNLVLFAAIRDRSYLSYGVFQILTVLLVLNGSGYTYEWFLSGYPMLDLSLTAPLG
                     MLTSVAIILFSADFLHIGKGLPRSVIRYFIAGLALFALLSFVIPLRVSIRYSSYFAVA
                     VCVLVFWLAIIRHRAGFGPARFFFVAFLPLLAFAFTFVISRFLTFIKTGPFSLHIFAA
                     GLPLAILLSSVLFSVALSDRFQTLRNALRKTMAAKDELRMRNEQALREEKHRINRELH
                     DVIGSDLSALIFELRKDHEHDSILPRLSRMLSRLRDLVHINALDPDFDLSSQIRSFLR
                     EIEMPGVRVEASIDEVALPVVSAFHLHRIFLESITNCLRHANATTLIIRLKRRHDKVT
                     LIVADNGQGFDVNKPGNGMTGVRERAQTIGAHLRIVSRSGKGTAVVLHISEAGAFLPL
                     DARGVLH"
     gene            3516..4442
                     /locus_tag="PKK76_07800"
     CDS             3516..4442
                     /locus_tag="PKK76_07800"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNL68413.1"
                     /translation="MLQYKLKSMSDWSDEAEPLASDLAAQILEGTKEGIIITDAESRI
                     VWVNPAFTSITQYTASECLGRRPSILASGSHGREFYEDMWRELSATGAWTGEIWNRKK
                     DGTIYPEWLSITRVSFRGEDRYVGIFSDISRRKAAEARIQYLAHFDALTGLPNRHFLV
                     DRANQVMSLCRRRRLPLSLMLLDLDYFKTINDKLGHSTGDEALKAVASRIQSIIRHED
                     TVARLGGDEYVILLPETNQDGAFHVASKILKMVTEPLRTGSELVHLSLSIGVAVFPEH
                     GASFEILCQNADAAMYEAKRAGGNKFRFFTLR"
     gene            4658..>6787
                     /locus_tag="PKK76_07805"
     CDS             4658..>6787
                     /locus_tag="PKK76_07805"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68414.1"
                     /translation="MIRASAFFLLFFATSLYTQPSQNRLEALYPGISSQIDRIGKLEP
                     DLASPSNRSALADYLQSNDPELRKGMKLILESELQSTMTRISSKLVVDGSDQDWKDVP
                     FVVTDPAGDNAADILEQRVFLDESGNLFIMMKPSLKAGAIPPEYIFQPLIYANGDRGS
                     FLKIYFAKGRARISERTGDKPEDLEIPGVEAAVGTVYEVKVPLDAWMRSKGGVGSLDV
                     WTGYFDRDKYDSVSQSIPILQTNYALLLLLDLIARGAVAGHDPITVALAYANSTLYAM
                     GDLDTRAQIRKDGREHFQLYRKVSAWQKSNSLPFELENLPVIPKMIWSARYNRLHHFG
                     LMREAIGLLGKKRLTKDIYLEFVDRPEVLEKIHDEIVARDLPLSRDITVLASNIEDLG
                     SSLCETRFALWKYEDLYKRGKMSKEDYDRIQAEAKNNYEIEYMGKKRNWEEFRWNNYQ
                     FQNRCFRGSKILGDCGTYTQAMTAIYRMAGLSSLVNQRVNQEATFSYPSTHNFPAYYH
                     PFLRRWVYIQVPDLSNAAYRPDRKMSLHLDLPVMNSLVFADGKAMFGGISTTEQHINF
                     LSMGYDAQKLEQFFISRDPSFLLLDRASAPDGKIMDSDSDGLPDLLELQLGTSPSMAD
                     TDGDGMSDGWEINRGLDPLRKDSAVDAIDGMLSPWEQSTLVSTTDPSGDGPDGSTDIL
                     WAGAKMDESNNLHLVVKAVDLPSSKTIF"
BASE COUNT         1721 a         1639 c         1757 g         1670 t
ORIGIN      
        1 ctttgatggg tgagaacggg ccgattaaag ttgcaaaatt taacttttta gtaaaagaaa
       61 acggagaggt ctggatcaat caagctgttg gaaacgagcc tggcgctgga gccaatgaaa
      121 cttcgaatca atggcagaac cctgttctcc tggacaaaat caaagtgcgc actgttgagt
      181 ttcctcgcga gctgaagaaa gaagggaacg gtttctatga ggacacgcct gaatccggcc
      241 cgatggttgt ccctcaagaa cgcaatgatc caactctatt gcagggcttc gtagaagcgt
      301 ctaacgtgaa cctggtttca caaatggtgc agatgattga agtacagcgt cagtatgagg
      361 caaaccagaa atcagtgacc acgcatgatc aattgctggg tcgcttgatc aatgaagtgg
      421 ctcgttagcg atttgcttcg gtttttatct tgacgactcc gcggtcctga ccgccattct
      481 tcatcggaat gaagaagagt tttcttgttt gcgctgtctg ggtcctggcc ttgagtctcg
      541 aaggcggatg cattcaatcg ccttccccga gcgcgaaccc ggatgctgtc atcttgcttc
      601 tgttcggttc ggtggccaca gtgcgtccag tgcaggccac gtgcaacaga actaactttc
      661 agtgctttga atacaatact ggttttaccg ggagccaaat gactgcagac tgcgcgagta
      721 tgtttcttgg agtactgagc gcgtcgggat gcccgacttc cagtcgcctg gggagttgct
      781 acattacaaa ccgcggatat gctaccaatg cgtttggaac aacagcagcg ttgcgctttg
      841 agactccggc gtgggcattc gggactgcgc aggggacctg tagtgcgtat ggcggcacgt
      901 tcgcacagaa ttgatctttc tgatttattt gccgaggagc attctttctc aggaaatcag
      961 tccgcgcgcg cgtcctttct caatggctcc tgatttgctt cggacttcga gtttacggta
     1021 cgtgttttct gtatagtttt tcagagtatt gatggatatt cccagcttat gcgcggcttc
     1081 cgcgtaggta agtcccatgc tcaagagctg caacacacgg cgttcctgtt gcgtcaagag
     1141 tccgtctgcc ctcgtcgatg gtggtgcgaa atgacccaga agtgctcttg ccattcgcgc
     1201 ggtgatcggt gctccgccca gatgaacact tcggatttca gcgagtaaca gttcggggga
     1261 agtgtctttt aaaagaaatc cgtttgcgcc ggcttccatg gcatgaatca agcgcgtttc
     1321 atcttcaaat gcggtgaaga taagcacctt catatcgcga agtgcctcgc tggatttcag
     1381 acgacgaaga acagacaatg cgttttgatc cggaagctca aaatctagca agagcacgtc
     1441 tgccgcttta tgaacgtcgc gaaagaaatc tgctcctgtg gaataagctc ctagaagttg
     1501 gaaatccgaa ttcgaggcga acatctctgc gagagtcctg gtgatgtttg gatcatcttc
     1561 gagtatcagg attcgaatcg ccatacctat tcttaatgca ggacgcccct cgcgtcaagg
     1621 gggagaaatg cccccgcttc cgaaatatgg agcactaccg ccgtgccttt gcccgaccgt
     1681 gaaacaatgc gaaggtgcgc gccaatcgtt tgcgctcgtt cgcgtactcc agtcatcccg
     1741 ttgcccggct tattcacatc aaacccctgg ccattgtcag caacaatgag tgttacttta
     1801 tcgtgtctgc gtttcaggcg aatgataagg gtggttgcat tcgcatgcct caaacaatta
     1861 gtaatgctct caagaaaaat gcgatgcaga tgaaatgcgc tgaccactgg aagcgccact
     1921 tcatcgatag aagcttccac acgcactcct ggcatttcaa tttctcttag aaaagagcga
     1981 atctgcgacg aaagatcaaa gtctggatcg agtgcgttga tatgaaccag atctctcaac
     2041 cgtgaaagca ttctgctcaa tctcggcagg atgctgtcgt gctcgtgatc tttccgtaat
     2101 tcgaaaatga gtgcggaaag atctgatcca attacatcgt gcagttcccg gttgattcgg
     2161 tgtttttctt ctctcagcgc ctgctcattt cgcatgcgaa gctcatcctt cgctgccatc
     2221 gttttgcgaa gagcgttgcg aagagtctgg aatcgatcag agagcgccac tgaaaacaaa
     2281 acgctactca gcaggatcgc cagaggcagg ccagcggcaa agatatggag agagaatggg
     2341 cccgtcttta tgaaggtcaa aaatcggcta atgacgaacg taaatgcgaa tgcaagaagt
     2401 ggaagaaacg ctacaaaaaa gaatctcgct ggcccaaaac cggcccggtg acgaatgatc
     2461 gcgagccaga acaccaacac acaaacggca actgcaaagt aagacgaata gcggatcgac
     2521 acacgcagtg gtatgacaaa ggacagaagt gcgaacaacg caagtccagc gatgaaatac
     2581 cgaatcacac ttctgggtaa gcccttgccg atgtgaagaa agtcagcgct gaagagtatg
     2641 atcgcgacgc tggtcaacat tcccaggggc gccgttagag aaagatccag catcgggtaa
     2701 ccgcttagaa accactcata cgtatacccg gatccgttga gcacgagtaa taccgtgagg
     2761 atttgaaaga ctccgtagga aagatagcta cgatcacgaa tggccgcgaa gagcacgaga
     2821 ttgaagattg ccagagagaa ctgaccgccc atggccaggc caatcaacat atcagaagtg
     2881 agctgattgt tacgcaactc ttcttctgac tgaatcgatg gtgcgaatcg aacaggtgtg
     2941 attcctttta cgcgaagata aaccgtggct gcgttcttcg tgcgaggaag gcgaaaggcg
     3001 atgtcagttg ttcttacatc gcgtttcccg atcattcccg actgcatttg tctgacgaga
     3061 gacccgtctg ttgcgacgta gatagaggca tcgtacattc tgaaattgaa catactcaga
     3121 aaatatacct gatcttctgg aactcccgcg caatcatatg aaaagcggaa ccaccagacg
     3181 ctgtctgtga agccatgatg gacggtcttt gaaccaacat tgtgaaatgt ctggctctgt
     3241 acatctgcga aagccaggga gccgctcgta tcttcgaatt cctccagacg ttctccaatg
     3301 taggtgcgtc ccacgagcga gttcaggcga atggggtttg cagcttctat ccccgaacag
     3361 aggaaacaga gaagaatcga atatcgcgcg atgaaagcgc gagaggttcc gttcaaatgg
     3421 ccctcgccat aggcgagagt gttttttgct gtgattctgt cactcgtttc gcaggatgca
     3481 ggggacattc tcccccttgc gtgcgatgag cccatgtgtt acagtacaaa ctgaagtcga
     3541 tgagtgattg gagcgatgaa gccgaacctc tggcgtcaga tctggcggcg cagatcctcg
     3601 aaggtaccaa ggaaggcatc atcatcacgg acgcggagag tcgcatcgtc tgggtgaatc
     3661 ccgcatttac aagcatcact caatacactg catctgagtg tctcggtaga agaccttcca
     3721 tactggcatc cggttcgcac ggtcgtgaat tctacgaaga catgtggaga gagctgagcg
     3781 cgactggcgc ctggactgga gagatctgga ataggaagaa ggacggcacg atttatccgg
     3841 aatggctctc catcacgcgc gtgagttttc gcggtgaaga ccgttatgtt ggaatctttt
     3901 ccgacatttc gcggcgcaaa gcagcggagg ctcgcattca atatctggct cattttgatg
     3961 ctctcactgg cttgccgaat cgtcatttcc tggtagatcg agccaatcaa gtcatgtctc
     4021 tctgccgcag gcgtcgtctt cctctttctt tgatgcttct tgatctggat tattttaaga
     4081 ccatcaacga taagttagga catagcacag gcgatgaagc actcaaagcc gtcgcgtcta
     4141 gaattcaatc catcattcgc catgaagata cggtggctcg acttggtggc gatgaatacg
     4201 tcattttgct tccggaaaca aatcaagacg gtgcgtttca tgtggccagc aagatattga
     4261 aaatggtcac ggaaccattg cgaaccggct ccgagcttgt gcacctgtcg ttgtctattg
     4321 gagttgctgt ctttcccgaa catggggcct cgttcgagat tctatgtcag aatgcggatg
     4381 cagccatgta tgaagcaaag cgagcaggtg ggaataagtt tagattcttt actttacgat
     4441 gaccaggggg aaatctttcc cctttcgctt gatcataaag cacctgggtc gttctgtaac
     4501 ctcggttcga atcttcctta gaaccacgcg aaatcgtgtt gtactcgacg agacagcaaa
     4561 acccatgacg attgttgtcc tctgacgcaa cgttaagtcc ttgcgagaac aaaagagaga
     4621 acccctctgg tcgtatccac gtctcagagg aaaagcgatg atccgcgcca gtgccttctt
     4681 tcttttgttt tttgcgacat ctctctatac gcagccttca cagaatcgtc tggaggccct
     4741 ttatccgggt atttcttcgc agatagatcg aattgggaaa ctggagcctg atctggcgag
     4801 tccctccaat cgcagtgctc tcgcggatta tttacagtcg aacgatccgg aattacgcaa
     4861 gggaatgaaa ctgattctgg aatcggagct ccagtctacc atgactcgaa tctcaagcaa
     4921 gcttgtagta gatggatccg atcaggactg gaaagatgtg ccgttcgttg tgacggaccc
     4981 ggccggagac aatgcagccg acatcctgga gcagagggtc ttcctcgatg aatcaggaaa
     5041 cttgttcatc atgatgaaac cttccctgaa ggccggggcc atcccaccgg agtacatttt
     5101 tcaacctctc atttacgcca atggagaccg cggctccttc ttgaagattt atttcgcaaa
     5161 gggcagagca cgaataagcg agcggacggg agataaacca gaagatctgg agattcctgg
     5221 tgtagaggca gcggtcggca cagtttatga agttaaggtt cctctcgatg cgtggatgcg
     5281 aagcaagggc ggcgtgggtt ctctcgacgt gtggaccgga tattttgacc gcgataagta
     5341 tgattctgtt tcacagtcga ttccgattct tcagacgaac tacgccttac tgctgttact
     5401 cgatctgatt gcacgaggtg ctgttgcagg tcacgatcca atcacggtgg ctctcgcata
     5461 cgcgaatagc acgttatacg ccatgggaga tctggacacg agagctcaaa ttcgcaaaga
     5521 cggacgtgag catttccagt tgtatcgtaa ggtgtccgcc tggcagaaat ccaattcgct
     5581 tccttttgag ctggaaaact tgcctgttat acccaagatg atctggagcg cacgttacaa
     5641 tcgtctgcac cattttggac tgatgcgtga agcaattggt cttctgggca agaaacgcct
     5701 aacaaaggac atctacctcg agtttgtgga caggcctgag gtccttgaga agatccatga
     5761 cgaaattgtt gcgcgtgacc tgcctctttc gcgcgatatt acagtgcttg catcgaacat
     5821 agaagatctt gggtcttccc tgtgcgaaac tcgctttgcg ctctggaaat acgaggacct
     5881 ctacaagagg ggaaagatga gcaaagagga ttacgatcgc atccaggcag aggcaaaaaa
     5941 caattatgaa atcgaatata tgggaaagaa gaggaactgg gaagagttta gatggaataa
     6001 ttatcaattc cagaatcgtt gttttcgcgg ttccaaaatt ttaggcgatt gcggcactta
     6061 cactcaggct atgactgcga tctatcgcat ggccggtttg agctccctgg tcaaccagcg
     6121 tgtcaatcag gaggccacat tctcgtaccc ttccacgcac aatttccccg catactacca
     6181 tcctttcctt cgcagatggg tttacatcca ggttccagat ctatccaatg ccgcctatcg
     6241 tccggatcga aaaatgtcat tgcatctgga tttgcctgtc atgaacagtc tcgtctttgc
     6301 agatgggaag gccatgtttg ggggaatatc tacaaccgaa cagcatatca attttctatc
     6361 tatgggctac gatgcacaga aactggaaca atttttcatc tctcgagatc catcctttct
     6421 tcttctcgat cgagcaagtg cgcctgacgg taaaatcatg gactcagatt cagatgggct
     6481 tccagatttg ctcgaattgc aattgggcac ttctccctcc atggcagaca cggacggaga
     6541 tggaatgagt gatggatggg aaatcaatcg cggactggat ccgcttagaa aagattctgc
     6601 tgtggatgcc atcgacggga tgctgagtcc gtgggaacag tctacgctgg tgagcacaac
     6661 agatccatcc ggggatggcc cggatgggag cacagacatt ctatgggccg gcgcgaaaat
     6721 ggatgaatcc aacaatctac acctcgtcgt aaaagcggta gacttgccgt cttccaagac
     6781 gatcttt
//
LOCUS       DAORFF010000152         6772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5783_length_6772_cov_4.086795, whole genome shotgun sequence.
ACCESSION   DAORFF010000152 DAORFF010000000
VERSION     DAORFF010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5783_length_6772_cov_4.086795"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..397
                     /locus_tag="PKK76_07810"
     CDS             62..397
                     /locus_tag="PKK76_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HNL68415.1"
                     /translation="MNIQIFGLKSCPNTRKAEMFFKERRITYQFINLAEKPMSRGELT
                     SVMASIPAEELIDTESRAYVSAGLQYMKFNIAEKLLEEPRLFKTPIVRDAKRATAGFA
                     PEIWKKWLS"
     gene            574..1818
                     /locus_tag="PKK76_07815"
     CDS             574..1818
                     /locus_tag="PKK76_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002124856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNL68416.1"
                     /translation="MQIDSGTTAWMLIATALVLLMVPGLAMFYGGLVRTKNVVGTMMH
                     SFVAMAVIGTLWVAVGYSLTFGKSIAGGIVGWNWDYFFLKGIDESVVNHVPEYVLAMF
                     QGKFAIITPALISGAMAERVSFRGYLAFIVLWFLIVYCPLAHWIWAADGWLATTGPLG
                     VIDLAGGLVIHVSAGTSALVVALFLGKRYGFPHNTQNPNNLVMTLMGAGLLWVGWFGF
                     NAGSTVQSGLDTARALTMTQISAAAGASTWMVIEAIKFRKVTSLGFVSGILAGLVVIT
                     PAAGVVLPSGALILGMCSSVLCFFALEMKNRLGYDDSLDCFGIHGVGSGMGVLALSFL
                     IRDSWLAKAAGIAGSWDHLAQFVVQLKGFGATVALSAVATIAICFVVEKTIGLRVSKS
                     EELAGLDHTEHRESGYGLINLN"
     gene            1842..2183
                     /locus_tag="PKK76_07820"
     CDS             1842..2183
                     /locus_tag="PKK76_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNL68417.1"
                     /translation="MKMIIAVIQPDKVDEVREALIAADITRISINRITGHGQQADTEL
                     FRGQEVTPNLLSKVEIKIACNDQFVDIIVQTIMKAARHGEGAIGDGKIFVLPLERCYR
                     IRTGESGNGAI"
     gene            2234..3094
                     /locus_tag="PKK76_07825"
     CDS             2234..3094
                     /locus_tag="PKK76_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNL68418.1"
                     /translation="MSQSDIVEYYSEAGQDYSAWSPAFNMHFGYYRKWMNPFRLEPML
                     EQMNAEILSRMTGGKPAREMEFLDAGCGLGATARHIARQASAKVLGITIVPWQIEEAG
                     KLAARAGLSVRITFDLRDYRDTKLPPNQFDGVYALESSCYAGAGDKADFIRESFRLLK
                     PGGRLVVADGFLSRLNSKPNAIYRKCLSLMEEFWALETFAHKEPFLAALEKAGYRDVQ
                     IENANFRIAPSVAFIPWVTAKFFIKDVIAKRRTLTRRQLGNALAPILGLIVGLHMHRF
                     SYCIVTARRP"
     gene            3140..4510
                     /locus_tag="PKK76_07830"
     CDS             3140..4510
                     /locus_tag="PKK76_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68419.1"
                     /translation="MQSQSSPISRTGPKWIMAAAAAGLLLVGGLVFYFVDFFPSLSHV
                     NVRLYSGATTGDYYAGAARSVQIAQSHQGKIENISTSGSIDNLKRLEQEREHGRFALV
                     QNGMPWPPGLQLVGHLPTQETVFLLGRGADRLTSFADLRGLRIGGGPVGSGTAYLSES
                     IFGMPSMKSLGVKLSFHPSDEQLRLIKEGKLDLGVFVISEKSEFIKKALLVEGLQIAG
                     LRGLESVTMNLAHLQARTMHQGLYDPVRNIPPVDKPVLSVNTLLVTNQEARRSEIVGM
                     LQVMSELYPNFINYNRTVVNETALNLPAGALDFYNNQGAEIFDRYLPRLMDLIPISNL
                     VQLVMGVSILFNLMGLANRFILWRIDANRIAIEEEIEQVFGSNVLPEEIEIMNPLPEH
                     AKGEGLHRLNALIGHLTGLESKCRKQSQSILVPMGAEMAYRYQEKLISTNLIAIKKYK
                     LKLERL"
     gene            4764..5375
                     /locus_tag="PKK76_07835"
     CDS             4764..5375
                     /locus_tag="PKK76_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68420.1"
                     /translation="MTRLTANLEAVTYLLGVPLGILFVIFQAGFSGQKLIIFLVSATV
                     GVLASMLYPVLRWRVLAPDINACIEGQVHGRQRRNLLRRLLNYPFWETIGVNIQWAGG
                     IAVVVLTNHLLEPLSMREFLPYVFVFIFLAPINAVSHYLAMQLSLAELIRSNRIFHGV
                     FLRRQDLVIVSLFRKTLGTMFSGVWLLLVFFGYTLYAALHWIH"
     gene            5335..6102
                     /locus_tag="PKK76_07840"
     CDS             5335..6102
                     /locus_tag="PKK76_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNL68421.1"
                     /translation="MDTPSTPLSIGYIDVSKLRLMTVAAAAQAVVLVAALSFVFATYL
                     NQFVLYLTRLLGRVASGDLTAKMTMASTDEIGALSTDFNRVTRSLRNARGQLESYATS
                     LEEKVQIRTEQLNATLSEVRTVKEKQDGDYYLTSLLLKPFLRAGLQTHSVEAAALISQ
                     FKKFEFRHFKEQIGGDSVTLDHVRLQERDYVFFMNSDAMGKSMQGVGGALVLGAVMHA
                     YLQRTKVSRQAHDVFPESWLNRLRRNCREFSRVSKGP"
     gene            6099..>6772
                     /locus_tag="PKK76_07845"
     CDS             6099..>6772
                     /locus_tag="PKK76_07845"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL68422.1"
                     /translation="MMISAIIGLLDGQTGCTYFINAEHPSPVLFREGQVTFAANGQSC
                     FKLGSPMQATSARVFTMRLLPGDVLICGSDGRDDLRLADSAGINTDETLFLRLVADAN
                     GDLNALEDTLRRTGEIMDDLSLIRIAYKGHRSEESEGSFDREIENFLKQTRRQRRKHV
                     DDLCRELKFEIALKLLEGAALKYPWELEMLGRLAYVLRKTGHLQRAADLGESIRLREP
                     DNADFL"
BASE COUNT         1683 a         1703 c         1778 g         1608 t
ORIGIN      
        1 gtaggaatca ttgccctcat cacatacatc gcgggacttg cgtaaagagc aggagagaaa
       61 ggtgaacata caaattttcg gactcaaaag ttgccctaac actagaaagg cggaaatgtt
      121 cttcaaggaa cgccgcataa cgtatcaatt catcaacctc gcggaaaagc caatgtccag
      181 aggagagctc acttccgtga tggcatccat accggcagaa gagctcatag acactgaatc
      241 cagggcgtat gtttctgcgg gacttcagta tatgaaattc aacattgcag aaaagctact
      301 ggaagaacca cggctcttca aaactcccat cgtccgcgat gcgaaacgtg ccacggcggg
      361 atttgcgcct gaaatctgga agaagtggct ctcttgatcc agagaatcag cgcctgaggc
      421 attttgccgc ataagagcgc agcatgcatc cagtctaatc agattgcctc tttttggatt
      481 gcgtccttcg cgcaagatac aaatttgccg gcgagcgttc cgaactggcg caagcacagc
      541 cacagcacgc ctcactctat tggaaggaag tttatgcaga tcgattctgg aactactgcc
      601 tggatgctca tcgccacggc cctcgtgctg ctgatggtac cgggcctggc catgttttac
      661 ggaggcctgg ttcggaccaa gaatgtggta ggcacaatga tgcacagctt cgtggccatg
      721 gctgtcatcg gaaccctgtg ggttgcggtt ggctattcgc tcacatttgg aaagagcata
      781 gcgggcggaa ttgtcggatg gaactgggac tacttcttcc tgaaaggaat tgatgaatcg
      841 gtagtgaatc acgttccaga atatgttctg gccatgttcc agggaaaatt tgcaatcatc
      901 actcctgctc tcatcagcgg tgcgatggcg gagagagttt ccttccgcgg ctacctggca
      961 ttcatcgtat tgtggtttct gattgtctac tgtcccctgg cccattggat ctgggctgca
     1021 gacggctggc tggctacaac aggcccgtta ggcgtcattg atctggccgg cggcctggtc
     1081 attcatgtat ctgctggaac gagcgcactg gttgtggcac tctttcttgg aaagagatac
     1141 ggcttcccgc acaacacgca aaatccaaac aacctggtga tgactctgat gggtgcgggt
     1201 ctcctgtggg taggctggtt tggattcaat gcaggatcca cagtgcaaag cggcctggat
     1261 acggcgcgcg cccttaccat gacgcaaata tcagcggccg caggtgcaag cacctggatg
     1321 gtgatcgaag caattaaatt tcgcaaggtt acttctcttg gatttgtatc cggaatcctc
     1381 gcagggctgg tggtgatcac tcctgctgca ggtgtagttc tcccttcggg tgcactgatt
     1441 ctcgggatgt gctcgagcgt actctgtttc ttcgcactcg aaatgaaaaa ccgtctgggg
     1501 tacgatgata gcctggactg ctttggaatc cacggagtgg gcagcggcat gggagtactt
     1561 gctctttcat tcttgattcg cgactcatgg cttgcaaaag ctgccggaat tgcaggatcg
     1621 tgggatcatc tagcacagtt cgtagttcaa ctgaaaggat ttggagcaac cgtcgctctt
     1681 tcagccgtcg cgacgatcgc catctgcttt gttgtagaaa aaacgatcgg attgagagtc
     1741 tccaagagcg aagagcttgc aggccttgac cacacagaac accgcgaaag cggctacggc
     1801 cttatcaacc tcaactaaca cggattaaac ggaattcgca aatgaaaatg atcatcgcag
     1861 ttatccagcc agacaaagta gacgaagtga gggaagctct cattgcagca gacatcacgc
     1921 gcatctccat caatcgaatc acgggccacg ggcagcaggc agacacagaa ctcttccgcg
     1981 gccaggaagt aacccccaac cttctctcga aggtggaaat caaaatcgca tgcaatgacc
     2041 agtttgtaga catcattgtg cagaccatca tgaaagcagc ccgtcacggt gaaggtgcga
     2101 ttggagacgg aaagatcttc gtcctgcctc tcgagcgctg ctatcgcatt cgcaccggcg
     2161 aaagcggaaa tggtgccatc taaccccgcg taatcgttct tgaaggtctg caggcaaccc
     2221 gcacacgtgg cgggtgagcc agtcggacat cgtcgaatac tattccgaag ccggccagga
     2281 ctacagcgcc tggagcccgg cgttcaatat gcacttcggg tattaccgca agtggatgaa
     2341 tccgtttcga ctcgagccaa tgctggagca gatgaacgct gagatcctga gcaggatgac
     2401 cggcggcaaa ccagcacgtg aaatggaatt tctggacgca ggatgtggcc tcggagccac
     2461 tgccagacac attgcacgcc aggccagcgc gaaagtcctt ggaattacga ttgttccctg
     2521 gcaaattgag gaagcgggta agctggcagc acgtgcaggt ctttctgttc gcataacgtt
     2581 cgatttaaga gactatcggg atacaaagct cccccccaat cagttcgacg gcgtctacgc
     2641 tcttgaatcc tcctgctatg caggagcagg agacaaagcg gatttcattc gcgaatcgtt
     2701 caggctactg aagccaggcg gacgattggt cgtggcagac ggtttcctat cgcgactgaa
     2761 ctcgaaacca aacgcaattt atagaaagtg tctttccctc atggaagaat tctgggcact
     2821 tgaaaccttt gctcacaagg agcctttcct tgcagctctt gagaaagcag gatatcgcga
     2881 cgttcaaatt gaaaatgcga attttcgcat cgcgccgtct gtagcattca tcccgtgggt
     2941 gactgcgaag ttctttatca aagatgtgat tgcgaagaga agaactctca ctcgtagaca
     3001 gctcgggaat gctctggcgc cgattcttgg tctgatcgtg ggactacaca tgcaccgatt
     3061 ttcctattgc atcgtaaccg cccgccgccc ctgatttttt cgattgattg cacaatgcac
     3121 tcttcatgat ggctttgcaa tgcaaagtca atcctcaccc atttcgcgca caggaccaaa
     3181 atggatcatg gcggctgccg ccgcgggact cctccttgtc ggagggcttg tgttttactt
     3241 tgtggatttc tttcccagcc tgtctcatgt aaatgttcgc ctgtattccg gcgccacgac
     3301 tggagattac tacgcaggcg cagcgcgatc tgttcaaatc gcacagtctc accagggcaa
     3361 aatcgaaaac atatccacaa gcggatccat agacaatctc aagcgactcg agcaagagcg
     3421 cgaacacggt cgattcgctc tggtgcaaaa tggaatgccg tggccgccgg ggctgcagct
     3481 ggtggggcat ctcccaacac aggagacagt gttcctcctg ggacgcggcg cggatcgact
     3541 tacctctttc gcagacctgc gcggattgcg aattggtgga ggccctgtgg gaagtggaac
     3601 tgcctacctc tctgaaagta tttttggcat gccatccatg aagtccctgg gtgtgaagct
     3661 gagctttcat ccttccgatg aacaactgcg tttgattaaa gaaggaaagc tggacctggg
     3721 cgtattcgta atcagcgaaa aatcagaatt catcaaaaag gcacttcttg tagaaggact
     3781 gcaaatcgcg ggccttcgcg gactggaaag cgtcacgatg aacctggctc atctgcaggc
     3841 acgcaccatg catcagggat tgtacgatcc agtgcgaaac attccccccg tcgacaaacc
     3901 ggtgttaagc gtcaatacat tgcttgtgac aaaccaggaa gcgaggagat ccgaaattgt
     3961 gggaatgctt caagtaatga gtgaactcta tccgaatttc atcaactaca accgcacagt
     4021 ggtaaatgaa acagcactca atcttccggc gggtgctctt gacttttata acaaccaggg
     4081 tgcagagatc ttcgaccgct acttgcctcg cctgatggat ctcatcccca tttcgaatct
     4141 ggtgcagctg gtgatgggcg tgagcattct attcaatttg atgggccttg cgaatcgttt
     4201 catcctctgg cgaatcgatg caaatcgaat tgcgattgaa gaagagatcg aacaggtgtt
     4261 tggatccaac gtgcttccgg aagaaattga aatcatgaac ccgcttcccg agcatgcgaa
     4321 aggagaagga ctgcatagac tcaatgctct tatagggcat ctcacgggac tggaaagcaa
     4381 atgcagaaag caatcgcaat cgattctggt tcctatggga gcggaaatgg cgtaccggta
     4441 tcaggaaaag ttgattagca cgaacctgat cgctatcaag aaatacaaac tgaagcttga
     4501 gcgtctttga aagaaaacaa tgaaccggca tcctcaaacc tgacgaacgt gactgaaaaa
     4561 caaataaacc tgcgacaaaa tgcggcgtgg acagtgtggg aaggataatc tttcacagat
     4621 tgaggagaat acagagataa tagtcgacag aaagaaaaga actgaccaca taacgcttaa
     4681 cgccacgggc taccgaccac aaagaccctg cacacaagca gcccgagcaa ctgagactcc
     4741 tcatgttaaa ccctcggaaa tttttgacaa gactcaccgc aaatctcgaa gcagtcacgt
     4801 atctactcgg tgttccgctc ggaatcctgt tcgtaatctt tcaagcgggt tttagcggcc
     4861 agaagctcat tatctttctc gtcagcgcga ccgtgggagt cctcgcgagt atgctttacc
     4921 cggttctccg ctggcgcgtg cttgcaccgg atatcaatgc gtgcattgag ggccaggttc
     4981 atggacggca acgacgcaat ttgctccggc gccttttgaa ctatccgttc tgggaaacca
     5041 tcggggtgaa catacagtgg gcgggcggca tcgctgttgt tgttctaacg aaccatttgc
     5101 tcgaaccact gtcgatgcga gagtttctgc cctatgtttt tgtgttcata tttcttgcgc
     5161 caatcaacgc agtgtcgcac tacctcgcca tgcagctctc tcttgcggaa ttgattcgca
     5221 gcaatcgaat ctttcacgga gtatttcttc gccgtcagga tctggtgatc gtttctcttt
     5281 tccggaaaac tctcggaact atgttttccg gggtctggct gttgctggtc ttctttggat
     5341 acaccctcta cgccgctctc cattggatac attgatgttt cgaaacttag actcatgaca
     5401 gtggctgccg ccgcccaggc agtggttctt gtcgctgcgc tttcgttcgt ttttgcaacg
     5461 tatctgaatc aatttgtttt gtatctgaca cgtttgcttg gccgtgtcgc atcgggagac
     5521 ctgacggcaa aaatgaccat ggcatctaca gatgaaatag gagcgctttc cacggacttc
     5581 aaccgggtca cgcgaagcct tcgcaatgcg cggggacagc tggagtctta cgccacgtct
     5641 ctcgaagaga aagttcaaat tcgcacagag cagctcaacg ctaccttgag cgaagtgcgc
     5701 actgtgaagg aaaagcagga tggagactac tatcttactt ctctgctgct gaaaccattc
     5761 ttgcgtgcag gattgcagac tcattcggtg gaagcagcgg cgcttatctc acaattcaaa
     5821 aagtttgagt tcaggcattt caaagaacaa attggcggag acagtgtcac gctcgatcat
     5881 gtgcgacttc aggaaagaga ctacgtgttc ttcatgaact ctgacgcaat gggaaaatcc
     5941 atgcagggcg tcggcggggc gcttgtactt ggtgcggtca tgcacgcata cttgcagaga
     6001 acgaaagttt cgcgtcaggc gcacgatgtg tttccagaaa gctggctcaa tcgcctgaga
     6061 aggaactgca gggagttttc gagagtttcg aagggtccat gatgatcagc gctatcattg
     6121 ggcttctcga tgggcaaact ggttgcactt atttcatcaa tgcagagcat ccttcacctg
     6181 tcctgtttcg cgaaggccag gttacatttg cggctaacgg tcaatcgtgt tttaaacttg
     6241 gatcgcccat gcaggcgact tccgctcgcg tgttcacgat gcgccttcta ccgggggacg
     6301 tcctgatttg cggctcggac ggccgtgacg atcttcgcct ggcggattcc gccggaatca
     6361 atacagacga aactttgttt cttcgactgg tagcggacgc gaatggagat ttaaacgcac
     6421 tggaagacac tctgcgtcgc acaggagaaa tcatggatga tctctctctc attcgaatcg
     6481 cctacaaggg acacagatct gaagaatcgg agggatcctt tgatcgcgag attgaaaact
     6541 tcctgaagca aactcgtcgc cagagacgca agcacgtgga tgacctctgc agagaactga
     6601 aatttgaaat cgccttgaag ctactggaag gggcagcact caagtatccg tgggaattag
     6661 aaatgcttgg cagactcgcg tatgttctga ggaaaacggg tcacctgcag agagcggcgg
     6721 acctgggaga atcgattcga ttacgagagc ctgacaatgc agattttctc tg
//
LOCUS       DAORFF010000153         6770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5787_length_6770_cov_3.521519, whole genome shotgun sequence.
ACCESSION   DAORFF010000153 DAORFF010000000
VERSION     DAORFF010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5787_length_6770_cov_3.521519"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PKK76_07850"
     CDS             <1..186
                     /locus_tag="PKK76_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68423.1"
                     /translation="GESVPRWDFDSAAKGQGDGSVLRADAIPPEPIQSEVYLSKYGHS
                     YLLNDPNAQDAIVKFLK"
     gene            complement(178..1488)
                     /locus_tag="PKK76_07855"
     CDS             complement(178..1488)
                     /locus_tag="PKK76_07855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNL68424.1"
                     /translation="MRTNIVHIGAGELQYEIRQIVEIGHNVMRLSGKPIAWENIGDPV
                     QKGEKLPDWMKDIIAQAVRDDSTYAYSPTRGLLETREFLAERLNREKGVKIHPDDIIF
                     FNGLGDAVNKVYGMLRREARVIGPTPAYSTHSSAEAAHAGYPPITYRLDPARGWMPDL
                     EELRNKVKYNESIAGIMIINPDNPTGAVFPRDVLEEMVEIARQYDLFLVGDEIYVNMV
                     FNGKKCTNLSEVIREVPGISLKGISKEFPWPGGRCGWMEVYNQASNPMFQKYVKSVLD
                     AKMLEVCSTTLPQVVIPRIMGNPEYVNWQKQRNAFFEKRSKRIAEILSGIPEIIVNPP
                     DGAFYVSIVFKKELNNRMKLNIPNAAVRDYIDGICSGNMEPDRRFVYHLLASRNICVV
                     PLTSFVCTLQGFRCTLLERDDAKFEEIYHGVRDAILEFLNSASL"
     gene            complement(1514..2317)
                     /locus_tag="PKK76_07860"
     CDS             complement(1514..2317)
                     /locus_tag="PKK76_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68425.1"
                     /translation="MMRACLLVSVALCAHSLWAQADPALPEPQSPSPTHYVSIHAGPA
                     VSASGSIVASSWAGLRLLELLVNSNVLYAPRISPALRYTGPIYKVPPLELSSRQYGFL
                     YERRMDHWFFGGALSYTDIKARLQIPETFVVAPNLGLNVPLVFRVTPEIHGRMLLASA
                     LQAEGSAAYHFWPEEVLDPYVRVFAGVGKGFVAHPLGGPYLHEIHAGTAVGLRWNRES
                     LFFGSEVNVIGHWVNSGPSSFIDRTNVLVNPGNGSIYIVRLLLSAGMRF"
     gene            complement(2420..3166)
                     /locus_tag="PKK76_07865"
     CDS             complement(2420..3166)
                     /locus_tag="PKK76_07865"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF002346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNL68426.1"
                     /translation="MSQSLEYLELQLYAGHLKGACSYLPEKESNLLFLEATGLGAGYR
                     LLLDRGYRRHGFHVYRPDCANCQECKILRLPVATFAPTRSQKRVWKKGQSVFRVETGP
                     PSYSREKEQLYCKYLACQHGSCDDMDPCRYSDFFVSSFLATTEELRLYAGDRLVGLGI
                     FDVVGDALSSVYFFFDPDYSRYSPGTFSILAEIDLCKARGLSHYYPGYFISACHAMNY
                     KARFGPAEVKLPAQEKWGPCVKSCLPWVDE"
     gene            3208..4290
                     /gene="serC"
                     /locus_tag="PKK76_07870"
     CDS             3208..4290
                     /gene="serC"
                     /locus_tag="PKK76_07870"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNL68427.1"
                     /translation="MRRVYNFSPGPAALPLPVLETIRDELLDYQNTGMSIMEMSHRGK
                     VFEGLLDDINQRLRKLASIPDRFDILYMTGGASAQFAFVPMNLSGKGRKAGYVNTGVW
                     SEKAIEQANLLGIQVFDAGSSKDKNHSYIPEVKTTSGLDYLHVTSNNTIYGTQFQKLP
                     SPGDTKLVIDMSSDFLSRPIDWSNIGMVYAGAQKNAGPSGVTIVVVDREYYGRESAET
                     PTVFRYSTYAKNNSLYNTPPTFQMYVFGLVVSWIEKQGGLEEMHRYNQHKAELIYNVM
                     DEFPKFYTGHAVKNARSLMNITFRLPSEDLEGKFLKAAEERKLMGLKGHRSVGGLRAS
                     TYNAVGEEACQVLSVLMREFARSNGG"
     gene            4292..4498
                     /locus_tag="PKK76_07875"
     CDS             4292..4498
                     /locus_tag="PKK76_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68428.1"
                     /translation="MLWLRLGDNELVQLDHVVSIKKQEPQAIELTFLEPNGKRILTFP
                     DPAERNRAFDRLLENLVKLRMALE"
     gene            complement(4552..5580)
                     /locus_tag="PKK76_07880"
     CDS             complement(4552..5580)
                     /locus_tag="PKK76_07880"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL68429.1"
                     /translation="MNGAYLLLGAMIGSAMMPRYPRFVYVTNFSSATVSMYTINQTTG
                     VLSANGTIAAGTQPRDIVVDPSGRFVYAANNGSNSVSMYSINQTTGSLTSIGTIAAGS
                     STQGLAVDPGGNFIYAVNNASNNVSMFTVNQATGMLSPNGTIAAGTGCLGIAVDPTGK
                     FAYVANNTSNNVSMYTINAVTGVLSANGTIGAGTGPMGVVADPTGKFAYVTNNASNNV
                     SMYTINPGSGVLSANGTIAAGTGPIGIASHSSGQFAFAANFTSNNLSMYTINTVTGVL
                     TANSTVAAGTGAQAIAVDPSGKFAYSGNGSANNVSMYSVNTSTGNLTSNGTIPAGSNA
                     IGIAIAYF"
     gene            complement(5951..6376)
                     /locus_tag="PKK76_07885"
     CDS             complement(5951..6376)
                     /locus_tag="PKK76_07885"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNL68430.1"
                     /translation="MKNALLWLEDHKDWGIEMLRMYLGVVLILKGFYFIRDMNSLLAL
                     IGLQSSLWLGGIFAHYIVLAHLIGGLFVIIGLLTRIVAAFQVPILLGACYVVFNHDGI
                     LSASSNFEFTSLVLILLVMFVFYGGGRLSVDYFLGKPKD"
     gene            complement(6507..>6770)
                     /locus_tag="PKK76_07890"
     CDS             complement(6507..>6770)
                     /locus_tag="PKK76_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNL68431.1"
                     /translation="IGSYEAFMSHLAYEYKQRSQQSVIRKLIRRTLTLGDTRIATVAP
                     GQMLGFYSILNFSARQADTLIHEGYMDARNQLSQFFGLDEAPY"
BASE COUNT         1794 a         1768 c         1667 g         1541 t
ORIGIN      
        1 ggcgaatccg ttcccagatg ggattttgac tccgcggcaa aaggccaggg agacggatct
       61 gtcctgcggg cagatgccat tccgcctgag cctatccaga gcgaagtcta cctttcaaag
      121 tacgggcatt cgtatctgct caatgacccg aacgcacagg acgcgattgt aaagtttcta
      181 aagtgaagcg ctgtttagaa attcgagaat tgcgtccctc actccgtggt agatctcttc
      241 gaatttagca tcgtcccttt cgagaagagt gcagcggaat ccttgcaaag tacatacaaa
      301 gctggtgaga ggcactacgc aaatatttcg agacgcaagc aaatggtaca cgaaacgacg
      361 atccggttcc atattccccg agcatattcc atcaatataa tctcgaacag cagcatttgg
      421 aatgttgagc ttcatgcggt tgttgagttc cttcttgaag acaatactca catagaaagc
      481 accgtccgga ggatttacaa tgatctccgg aattccagac agaatctcag caatgcgttt
      541 gctccttttt tcaaagaatg catttctttg cttctgccag ttcacgtact ctggattccc
      601 catgatcctt ggaatcacta cctgcggaag agttgtggaa catacttcca gcatctttgc
      661 gtccagaact gacttcacat acttctggaa cattggattg cttgcctggt tgtatacttc
      721 catccagccg caacgtcctc ccggccaggg aaactctttg gaaatccctt tcagggaaat
      781 gcctggtact tctcgaatca cttctgaaag attcgtacat ttcttcccgt taaaaaccat
      841 gtttacgtag atttcatctc cgaccaggaa cagatcgtac tgacgcgcaa tctccaccat
      901 ttcttccaga acatctcgag gaaacactgc tcccgtagga ttgtccgggt tgatgatcat
      961 gatgcctgca atgctttcgt tgtacttcac cttgttgcga agttcttcca ggtcaggcat
     1021 ccatccgcga gcaggatcga ggcgatacgt aatcggagga taaccagcat gtgccgcttc
     1081 agcagaagaa tgggtggaat aggcaggagt aggcccgatt acacgagctt cgcgacgaag
     1141 cattccatag accttgttga ctgcatctcc cagcccatta aagaaaatga tatcgtctgg
     1201 atgaatcttg actccctttt cacgattgag tctttctgcg agaaactcgc gcgtttctaa
     1261 aagaccacgg gtgggactgt acgcgtaagt gctatcgtcg cgaaccgcct gggcgatgat
     1321 atctttcatc cagtccggga gcttttctcc cttctgaact ggatcaccga tgttctccca
     1381 cgcgatcggc ttgcctgaaa ggcgcataac gttgtggccg atctcaacga tctgcctgat
     1441 ttcatactgg agctcacctg ctccaatatg tacgatattc gttctcatga cattcctggc
     1501 cctctccggc cgactagaag cgcatgcccg cgctcaaaag aagacggaca atgtagatgc
     1561 ttccattgcc aggattcaca aggacgttcg tgcggtcaat gaagctcgaa gggcctgagt
     1621 tcacccagtg cccgattacg ttgacttcac tcccaaaaaa cagactttct ctgttccatc
     1681 tcagacccac tgcagtcccg gcgtgaattt cgtgaagata gggccctccc agcgggtgag
     1741 ccacaaagcc cttgcccact ccggcgaaga cgcgcacata tggatcgagt acttcctctg
     1801 gccagaaatg ataggcagca cttccttccg cctgaagagc agaggcaaga agcattcgcc
     1861 cgtgtatctc aggagtaacg cgaaacacca gaggaacatt caaccccaga ttgggcgcca
     1921 ccacgaacgt ctccggaatt tgcaggcgtg cctttatgtc cgtatagctg agagctcctc
     1981 cgaaaaacca gtggtccatg cgtctttcgt ataaaaaccc gtactgtctg gaggataact
     2041 ccagaggtgg aactttgtag attggcccgg tataacggag cgcaggcgaa atgcggggag
     2101 catacaatac attagaattc acaagcaatt ctaaaaggcg aagccctgcc caggaactgg
     2161 ctacaatcga tccggatgcg ctgacggcgg gacctgcgtg aatcgagaca tagtgagtgg
     2221 ggctcggact ctggggttcg ggtagagcgg gatctgcctg ggcccacaga gaatgcgcgc
     2281 aaagcgcaac agaaaccaga aggcaagcgc gcatcatcgg agcatcagga aacagtgacg
     2341 ttcaaggaat agaaaaaaaa gggggcttac gccccccgcc tttcatttag caaagaaaag
     2401 ctaataatca atcactggtc tactcatcga cccaggggag acacgactta acgcacgggc
     2461 cccacttttc ctgggcagga agcttcactt ctgccggccc aaaccgtgcc ttgtagttca
     2521 tggcatggca ggcagagata aaatatccag ggtagtaatg cgaaagtccg cgagccttgc
     2581 agagatcgat ctctgccaga atggaaaagg tgccgggact atagcgggaa tagtccggat
     2641 caaagaaaaa atacacggag gaaagggcat cccccactac atcgaaaatg ccaaggccca
     2701 ccaggcgatc tcctgcatag agcctcaatt cctccgtagt cgcaagaaaa ctgcttacaa
     2761 agaaatcgga atatctgcaa ggatccatgt cgtcgcagga tccgtgctgg catgccagat
     2821 acttgcagta gagctgttcc ttttctctgg aataagaagg cggcccggtt tccactctga
     2881 aaacagactg acccttcttc cagactctct tctggcttcg cgtgggtgca aacgtggcca
     2941 caggcaggcg gaggatcttg cactcctggc agtttgcgca gtccggacga tatacgtgga
     3001 atccgtgcct tctgtagccg cggtcgagca atagcctgta acccgcgcca aggcccgtcg
     3061 cctcaagaaa gagcaaattc gattctttct ccggcaaata ggagcatgcc cccttcaggt
     3121 ggcctgcgta taattgtaat tcgagatatt ccagggactg gctcatttgg ctttgactga
     3181 gagctctact ttagaaaatc agacgaaatg agacgagtct ataacttttc tccaggtccg
     3241 gccgctcttc cgctgccagt tctggaaacc atccgcgatg agctgctcga ttaccagaat
     3301 acagggatgt ccatcatgga aatgtcccac cggggtaagg tttttgaggg cttgctggat
     3361 gatatcaacc agagactgcg caaactggca tccatcccgg atcgctttga tatactgtac
     3421 atgacaggcg gggcatctgc ccagttcgct tttgttccca tgaacctctc aggcaaaggc
     3481 cgcaaagcag gctacgtcaa taccggagtc tggtccgaaa aggcaatcga acaggccaat
     3541 ttgctcggaa tccaggtctt tgacgcaggg agctccaaag ataagaacca cagctacatc
     3601 cccgaggtaa agaccacctc aggcctggat tacttgcatg tgacctctaa caacacaatc
     3661 tacggaaccc agttccagaa gctccccagt cctggagata caaagctcgt catagacatg
     3721 agctctgact ttctatctcg tcccattgac tggtctaaca tcggaatggt atacgctggc
     3781 gcgcagaaga atgcgggtcc atccggagtg accatcgttg tagtggatcg tgaatactat
     3841 ggaagagagt ctgcagaaac tccaactgta ttccgctaca gcacgtacgc gaaaaacaac
     3901 agcctctaca acactcctcc cacctttcaa atgtacgtgt ttggtctggt ggtttcctgg
     3961 attgaaaaac agggcggcct ggaagagatg cacaggtaca accagcacaa ggctgaactg
     4021 atctacaatg taatggacga atttcctaag ttctacacag gacatgcggt gaagaatgcc
     4081 agatctctca tgaacattac gtttagactt ccatcagaag acctcgaagg aaagttcttg
     4141 aaggcagcag aagaaagaaa gctcatggga ttgaaaggtc accgctcagt aggcggactg
     4201 cgcgcttcca cctacaatgc agtaggcgaa gaagcctgcc aggttctctc cgtattgatg
     4261 cgtgaatttg caagatcgaa cggaggatag gatgctgtgg ctcagacttg gtgacaacga
     4321 actcgttcaa ctggatcacg tcgtttccat caagaagcaa gagccacagg ccatcgaact
     4381 aacttttctc gaaccaaacg ggaagcgcat cctcaccttc ccggatccag cagaacgcaa
     4441 cagagcgttt gacaggctcc ttgagaacct ggtgaagctg aggatggctc tggaataatc
     4501 agccgcactt tttcttttga agaattcgcg ctgcgaaaac gctgcggttt ctcagaaata
     4561 ggcgatcgcg atgccaatgg cgttcgatcc tgcgggtatt gtgccatttg aagtcaggtt
     4621 gcccgtggaa gtatttacgg agtacatgga cacattgttg gcagatccgt ttcccgaata
     4681 agcgaacttt cctgatggat ctaccgcaat cgcttgagca ccagtgcccg cagcaaccgt
     4741 actatttgcc gtaagaactc ccgtgacggt gttgatcgtg tacatagaaa gattattgga
     4801 agtaaagttc gcagcaaaag caaactgccc gcttgaatga gatgcaattc ctatggggcc
     4861 tgttcccgca gcgattgtac cattggccga gagaacgcct gaacccgggt tgattgtgta
     4921 catggacaca ttgttcgaag cgttgttcgt aacatacgca aacttacccg tcgggtctgc
     4981 aacaacaccc atgggccctg tcccggcacc gatcgtgccg ttcgcggaca gaactccggt
     5041 caccgcattg attgtgtaca tggacacatt attcgaagtg ttgtttgcca catacgcaaa
     5101 cttacctgtt ggatctacag caatgcccag acaacctgtt cccgcagcga tcgtaccgtt
     5161 tggcgaaagc atgccagttg cctgatttac ggtgaacatg gagacgttgt ttgacgcgtt
     5221 gttgactgca taaataaaat tgccaccggg atccactgct aagccctgag tgctcgaacc
     5281 cgcggctatg gttccaatag atgtgagaga tccagttgtc tggttgatgg aatacatcga
     5341 tacgctattg gagccgttat tggcggcata cacaaatctt ccgctcggat ctacgacaat
     5401 gtctcgaggc tgagtacccg ccgcgatagt accgtttgcg gaaagcacac cagtcgtctg
     5461 attgatcgta tacatcgata cggtggcaga tgagaagttg gtcacataca caaatcgcgg
     5521 ataccgcggc atcatcgcgc ttccgatcat cgcgccgagc agtagatatg cgccgttcac
     5581 atctctattg cttatgggga aagagcccgt acacgagaaa gcgaaaatta gagacgtttg
     5641 agccaccgct gcatatgccc ttgcggcgga acctgagagc cggactatgc gctttgagat
     5701 cgatcttccc gacaagcaac gcatacttca ttctcgcgtg tttttcgggt caggtcaaca
     5761 agaaaaggtt gattgcgcag cacggaaaca tctcaacgcc ccaatccggt tcaagggcac
     5821 gttgaaattg agaccacgca cggaaagaga ggcattcttt caggtgcgca ggattttcgt
     5881 gcgaattttc gcgcgcattc ccatgtgaag aatttcgcac cgcgaaattc tctcaatcgc
     5941 cggcgccaca tcagtccttt ggtttgccca ggaagtaatc cacggacaac cttcccccac
     6001 cgtaaaacac aaacatcaca agcaaaatca gcaccaggga agtgaattcg aaattcgagg
     6061 aagcggacag aatcccatcg tggttgaaca ccacgtaaca ggcacccagc aaaatcggca
     6121 cttgaaacgc agctacgatc ctggtcagaa gcccgatgat cacaaacaga ccaccgatca
     6181 ggtgggccag aacaatgtag tgagcgaaaa ttccacccag ccacaaagag ctttgcaggc
     6241 caatgagagc gagcaaagaa ttcatgtcac gaatgaagta aaaacctttg agaatcaaaa
     6301 ctactccgag gtacatacgg agcatttcga ttccccagtc tttgtgatct tccagccaga
     6361 gcagcgcatt tttcatacag cctcccggtg ccgggggcgc aatgcagcag agcctcaggt
     6421 gcatccgcaa aaccaggcac cctgaacgcc ctcagtataa ctcgacccta aaaggaggca
     6481 agattaaatt atgaatttta agatcactag taaggcgctt cgtccaggcc aaaaaactgc
     6541 gatagctgat tgcgcgcgtc catgtaaccc tcatgaatga gagtgtccgc ctgacgggcc
     6601 gagaaattca aaatagaata aaatccaagc atttgccctg gagcaaccgt tgcaatgcga
     6661 gtgtccccca gagtgagagt tctacgaatc aatttgcgta ttacgctctg ctgcgatctt
     6721 tgtttgtact cgtaggcgag atgggacatg aatgcttcat aggatccaat
//
LOCUS       DAORFF010000154         6745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5817_length_6745_cov_3.575934, whole genome shotgun sequence.
ACCESSION   DAORFF010000154 DAORFF010000000
VERSION     DAORFF010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5817_length_6745_cov_3.575934"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKK76_07895"
     CDS             complement(<1..463)
                     /locus_tag="PKK76_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68432.1"
                     /translation="MIYKKFMRAGGSVALIFSGFTFSLEAQNPTEEDKRNNVTQPAPA
                     TPAPAPDAIPDGAVDVQLKFTSNFLDRGEDRGRNRSVQRGKAYNSKEDPWFFQPSAKI
                     KVPGSGLYFNAAGSFALSNRNDKDVDQKLEDSPTGEQTLLTDSVNPLVNTAR"
     gene            619..1770
                     /locus_tag="PKK76_07900"
     CDS             619..1770
                     /locus_tag="PKK76_07900"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HNL68433.1"
                     /translation="MPKQLLIIPQAILITISCLKPQPVSEAAVVRDYSLLVHASYLQA
                     RKDAQAMQKSVSDFLAAPGDAGLKSARDSWIKARVSYSQTEAFRFYGGPIDRPKSQGK
                     EEGPEALLNSWPVNESYIDYVKGNPGAGIINDPRIQINATTLTGKNQQKDEADVATGF
                     HAIEFLLWGQDFNPAGPGDRPVSDYTTKSNHERRKQYLRVTTDMVVTQLRSLEEDWAP
                     GKENNYAAEFQKLNSKVALGHILTGLVTLAGFELASERTATALKSGDQEDEQSCFSDN
                     THNDFINNIQGILNVYEGRVGDYSGIGLDAWIKSASPEMADQILEALRSTKDKAISLP
                     VPVDSILASPKNSDSRQKLESLSHALAQDAKLFQKAGILLGVDARLIAE"
     gene            1779..3158
                     /locus_tag="PKK76_07905"
     CDS             1779..3158
                     /locus_tag="PKK76_07905"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNL68434.1"
                     /translation="MCKGFPIVLVLLSLSECRRARDPGLQYSGGETTVFVTSSLAFSS
                     PAANLDVDKMPAFTFGNRVFNTNWVTAPSSTKNFDGLGPFFNRVSCSSCHLLDGRGQP
                     PETPKDTMDSMLVRVSLPGESEVGGPRPHPIYGDQINDRAILGVKPEGRITVRYVEIA
                     GNYPDGKHYDLRRPVYSFRLNYGDAPGILFSPRVAPAIHGLGLLEAIPEAAILAIARL
                     QASSGIQGKPNYVFNALTGGKSLGRFGWKANVPTLRQQNAGAALGDIGLTSEIFPREN
                     ITAIQKDNKALASVAGLGGDSVDLKTEFLDDLTFYTQTLGVPARRNHKDPEIRKGEQI
                     FSQIGCSGCHVPTFETGEHPAGIKEVSHQVVHPYTDLLLHDMGSDLADGRPDFQASGS
                     EWRTPPLWGIGLVEAVNRHTTLLHDGRARNLEEAILWHGGQAARAREAFKNMSEKERT
                     LLLRFLKSL"
     gene            complement(3174..4220)
                     /locus_tag="PKK76_07910"
     CDS             complement(3174..4220)
                     /locus_tag="PKK76_07910"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNL68435.1"
                     /translation="MNTRALLLKVAIFVTLSAAVACKKDQVDLQGAAITALQGSVSVT
                     DNKGTNKTISPSSLYTKDGILMPGYVIQTGRDGQVDLQFSPTMKLRMGPDTTLKIESA
                     RILASKGFEQLQLRLEKGRVFLASEKLAAASRINVVTPTAIASVRGTEWVVKEENGKS
                     QTMVKEGKVEVADDTLKDSQVVEENKKAEVQSDGKITVSTQTDSDKKEVADLGRDLAS
                     ITENGRNEIQSLIQQFQEQKILIQQTIEEQKKSNEEIMQQQKDSNQELMKDQHKKDLE
                     NLREQIQRDREEMDKLKSQLGADKDAMTQGGREEKDKISNQGRDEINQMKQNSPLSTQ
                     DQNKAELERLRKGQ"
     gene            4320..4832
                     /locus_tag="PKK76_07915"
     CDS             4320..4832
                     /locus_tag="PKK76_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNL68436.1"
                     /translation="MKKILTFVDDIYEDLELWYPKLRVEEAGWSVDLAGPEIKKYSGK
                     HGYPASSNIKISDVQEDYYQALLIPGGFMPDKLRRDARVLSITKNFFDSGKLVAFICH
                     GGWIPISAKILNGKKATGSRGIKDDLENAGAIWVDEPVVRDGNLISSRTPVDLPAFGR
                     AITEYLADHS"
     gene            4829..5461
                     /locus_tag="PKK76_07920"
     CDS             4829..5461
                     /locus_tag="PKK76_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNL68437.1"
                     /translation="MKDSYLRLLENNKIWAAEKIKENPKYFQHLAELQTPEFLWIGCS
                     DSRVPANEITGTDPGEIFVHRNVANLVVHTDLNMLSVLQFSVEVLKVKHVIVCGHYGC
                     GGVKYAMDTQDHGLVNKWLRNIKEIHVKYDEQLSKLTDVRARADRLVELNVIEGVRSL
                     AKTSIVQKAWKTRSLQIHGWVYGLENGLIKDLNVMVNGIADLEPIFRLDV"
     gene            complement(5635..6300)
                     /locus_tag="PKK76_07925"
     CDS             complement(5635..6300)
                     /locus_tag="PKK76_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL68438.1"
                     /translation="MANMLDFQFEGMRLKYIKKGSGRPLLLFHANGFSAGSYAPLTDH
                     LANHGFHVHALNFMGHGGSDSTTDFHNWEFFGRQAAAFAEHLGYERFFAVGHSIGGAS
                     LLLATPSMEGRLVAGALLDPTVFSPLTSRFASLIPNPLAALAEKRRREFKDLRVVSRS
                     FRMNPLFKQWHPASFEGYLETAFEATNAGYRLCLDQKLRRKYFEAFMRASGESFALSR
                     NPF"
     gene            6408..>6745
                     /locus_tag="PKK76_07930"
     CDS             6408..>6745
                     /locus_tag="PKK76_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNL68439.1"
                     /translation="MPNQITKSEALRFISDITHAINQSDNAENVLERILSACIHATHA
                     ESGSIMLIDEDGENLTIHVSQGLAPESAHLKLRIGEGITGNVARTGKARIVGDATSES
                     DYIPVKEGLIS"
BASE COUNT         1628 a         1620 c         1751 g         1746 t
ORIGIN      
        1 atcgagccgt gttgacaaga gggttcacgg aatcggtcag aagggtttgc tcacctgtcg
       61 gactgtcttc gagtttctga tccacgtctt tgtcgtttct gttcgaaaga gcaaaagatc
      121 ctgccgcgtt gaaataaagg ccggatccag ggactttgat tttcgcagat ggctgaaaga
      181 accaggggtc ttcttttgaa ttgtaggcct ttcctctctg cacgcttctg ttgcgccctc
      241 tgtcttctcc tctatccaga aagttcgacg tgaacttcag ctgcacgtcc acggctccat
      301 ccgggatcgc atctggtgcc ggcgcaggtg tagccggggc gggctgggtt acattgttcc
      361 gtttgtcttc ctccgttgga ttctgcgctt cgagcgaaaa tgtgaagcca gagaagatca
      421 aagccacgct tccacctgcg cgcatgaatt ttttgtaaat catggattct ccttttggtc
      481 ccgctttgcg ggactctggg ccatatctgg gaggtgccga ttccagtcaa acagatattg
      541 agactcattc gcaatatcat ttgaccgccc agggctggct gattctctgg tcaaaatcta
      601 tccatccagg agcaaacggt gccaaagcaa cttctgatta ttcctcaagc gattctcatc
      661 acaatttcgt gtcttaagcc tcagcctgtc agcgaggctg cagtggtacg tgattatagc
      721 ctccttgtgc acgcttctta tcttcaggct cgcaaagacg cgcaggccat gcagaaatca
      781 gtcagcgact ttctggctgc tccgggcgat gcaggattga agtctgcaag agattcgtgg
      841 atcaaggcga gagtctccta cagccagacg gaagcgtttc gtttttacgg cgggcccatt
      901 gatcgcccta aatctcaggg aaaagaagaa ggcccagaag ctcttctcaa ttcatggccg
      961 gtgaatgaat cctatattga ttacgtgaag ggcaatccag gagcagggat catcaacgat
     1021 cccaggattc agatcaatgc aaccacgtta accggcaaga atcaacagaa agatgaggcg
     1081 gacgtggcca ctggatttca cgcgattgag tttctactct ggggccagga tttcaatccg
     1141 gcgggtccag gggatagacc tgtgagtgat tacactacga agtcgaatca tgagcgtcgc
     1201 aagcagtatc ttcgcgtaac cacagacatg gttgtaacgc agctgcgatc tctggaggag
     1261 gattgggcgc ccgggaaaga aaacaactat gccgctgaat tccaaaaact gaactcgaaa
     1321 gtcgctctcg gtcatatact gactggactt gtgactctgg ccggctttga gcttgcgtcc
     1381 gagagaacgg ccacggcact gaagagtgga gatcaagagg atgaacaatc ttgcttcagc
     1441 gataacacgc acaatgattt cataaataac attcaaggaa tattgaacgt ttatgagggt
     1501 agggtgggcg actactccgg aataggactc gatgcgtgga tcaaatctgc ttctccggaa
     1561 atggcagatc aaatcttaga agcacttcgc tccacgaaag acaaagcaat ttctctcccg
     1621 gttcccgtag actcaattct cgccagtcca aaaaactcag actccagaca gaagcttgaa
     1681 agtctatcgc atgcgcttgc tcaagatgcg aaactgtttc agaaggccgg tattcttctt
     1741 ggagtagatg ctcgtttgat agccgaataa ccatggttat gtgcaaagga tttccgattg
     1801 tactggtcct tttatctttg agcgaatgcc gccgtgccag agatccggga ctacaataca
     1861 gtgggggcga aaccactgtt tttgtcacat cctcgcttgc ctttagttct ccagccgcga
     1921 atttagacgt ggacaaaatg cccgcgttca cgtttggcaa tcgcgtcttc aatacgaact
     1981 gggtcactgc tccctcctca acaaagaact tcgatgggct cggacctttt ttcaatcgag
     2041 tttcatgttc gagctgtcat ctattggatg gccgcggtca acctccggaa acgccgaaag
     2101 acacgatgga ttcgatgctc gtccgcgtga gcttacccgg agaatccgag gtgggaggac
     2161 ccaggcctca tcctatctac ggagatcaaa tcaatgatcg cgccatcctg ggagtgaaac
     2221 cggaaggtcg cataacggtt cgatatgtgg agatcgccgg aaactatccg gatggaaagc
     2281 actacgatct tcgtagaccc gtatattcat tccgattgaa ctacggggac gcgcccggga
     2341 ttctattttc accgcgagtt gcgcctgcga ttcatgggct tggtctcctg gaagcaattc
     2401 cggaggccgc gattctggcc atcgctcgtc tccaggcttc ctccggaatc cagggcaaac
     2461 ccaattatgt tttcaatgct ctcacaggcg gaaaatctct cggacgattt ggctggaagg
     2521 ccaatgttcc tactttgaga cagcagaatg cgggagcagc gttaggcgac attggtctga
     2581 caagtgagat attccctcgc gaaaatataa ccgccatcca aaaggacaac aaggctctgg
     2641 cgtctgtggc cggtttgggt ggcgattcgg tagatttgaa aacagagttt ctggatgatt
     2701 tgactttcta tacacagacg cttggtgttc ctgcgcggcg aaatcataag gatccggaaa
     2761 tccggaaggg agagcagatc ttttctcaga tcggatgctc tggttgtcac gtgcccactt
     2821 tcgagactgg cgagcatccg gccggaatca aagaagtcag tcaccaggtg gttcatcctt
     2881 acacggatct attgttgcac gatatgggat cggatctggc agacggcagg ccggactttc
     2941 aggccagcgg cagtgaatgg cgcacgccgc ctctctgggg cattggactt gtggaggccg
     3001 tcaatcgcca cactactctt ttgcatgatg gacgtgcccg gaatctggaa gaagccattc
     3061 tctggcacgg cgggcaggca gcccgagcca gagaggcttt caagaatatg tcagaaaagg
     3121 agcggactct ccttcttcga ttcctgaaaa gcctgtaatg gaatgcgaaa gggttattgc
     3181 ccctttcgca acctttctag ctccgccttg ttctgatcct gcgtactgag tggagagttt
     3241 tgtttcattt gattgatttc atcgcgtccc tgattcgaaa tcttgtcttt ttcttcgcgg
     3301 ccgccttgag tcattgcgtc tttgtctgca cccagttgtg atttgagttt gtccatttcc
     3361 tcgcgatcgc gctggatttg ttcgcgaaga ttttcgagat cctttttgtg ctggtccttc
     3421 attagctcct gattgctatc cttttgctgt tgcatgattt cttcattgga tttcttttgt
     3481 tcttcgattg tttgttgaat taggattttc tgctcctgga attgctggat cagactctga
     3541 atttcattgc gaccgttttc agtgatggat gccaggtcgc gccccaggtc tgccacttcc
     3601 ttcttatcag aatctgtctg cgtggaaaca gtgatctttc cgtctgattg cacttctgct
     3661 tttttgttct cttcaacaac ttgcgaatct ttgagtgtgt catcggcgac ttcaactttg
     3721 ccttctttga ccatggtttg actcttgcca ttctcttctt tcacaaccca ttctgtcccc
     3781 cgaacggaag caatcgcagt cggagtgact acgttgatac gggaagcagc ggcgagtttc
     3841 tcacttgcga ggaacaccct tcctttctca aggcgcagtt gcagttgttc aaatcctttg
     3901 ctggcaagaa tgcgcgcaga ttcgatcttc agtgttgtgt ccggacccat gcggagtttc
     3961 atggttggtg aaaattgcag atcgacctgt ccgtcccggc cggtttggat gacgtatcca
     4021 ggcatcagga ttccatcttt ggtatagaga gaagaagggg agatggtttt gttcgttcct
     4081 ttgttgtctg tgactgacac ggatccctgc agtgccgtaa tggccgctcc ctgtagatcc
     4141 acttgatctt ttttgcacgc gactgcggca gatagcgtca cgaagatagc aactttcagt
     4201 aggagagctc gagtattcat tgacgcaggg gatcattcgg ccttcgcttt gacgacaaaa
     4261 aaactcccgg gcgtttgttc aaaatcaatc tttgtcagag ataaccgttg gttgaaatca
     4321 tgaagaaaat acttacattt gttgatgata tttatgaaga tctggagctc tggtatccga
     4381 agcttcgggt cgaagaggct ggatggagtg ttgaccttgc gggtccagag attaagaaat
     4441 attcagggaa gcacggatat cctgctagct cgaacatcaa gatttctgac gtgcaggaag
     4501 attattatca agcgcttttg attccaggcg gcttcatgcc ggacaagttg cgccgcgatg
     4561 cgagagtact ttccatcaca aagaactttt ttgattcagg caaactggtc gctttcatct
     4621 gtcatggtgg ctggattcct atttctgcga agattcttaa cggaaagaaa gcgaccggat
     4681 cccgcggaat caaagacgat ctggaaaatg cgggcgctat ctgggtggat gaacctgttg
     4741 tgcgtgatgg aaatttgatt tcaagccgaa cccccgtgga tttgcctgcc tttggacgag
     4801 ctatcacaga atacctggcg gaccactcat gaaagattct tacttaagat tgctagaaaa
     4861 taacaagatc tgggccgcgg aaaagataaa agagaatcca aagtattttc agcatctagc
     4921 ggaactgcag accccagaat tcctctggat tggttgttcg gatagccgcg tacctgccaa
     4981 tgaaatcacc ggaacagatc cgggagaaat ttttgttcat cgaaatgtcg ccaaccttgt
     5041 cgtgcataca gatttaaaca tgctcagcgt actgcaattc agcgtggaag tgttgaaagt
     5101 gaagcacgtg attgtgtgcg gacactacgg atgtggtggt gtgaagtatg cgatggacac
     5161 tcaggatcac ggacttgtga ataagtggct cagaaacatt aaagaaattc acgtgaagta
     5221 cgatgagcag ctttcgaaat taaccgatgt gcgagcacgc gcggatcgtc tggtagaatt
     5281 gaacgtgatt gaaggcgtgc gcagtctggc aaagaccagc atcgtacaga aagcgtggaa
     5341 gacgcgcagc ctgcaaatcc acggttgggt gtacggtctg gagaatgggc tgatcaaaga
     5401 cctcaacgtg atggtgaacg gaatcgcgga ccttgaacct atcttccgtc tcgatgtttg
     5461 agatttattc ctgcgcgtca aagaatcgcg caatctctct accaacccct tccgggtctt
     5521 ccatcgggaa acagtgagtg ccggagtgtt tgatccatct ggatttggta tgagatttca
     5581 gcaagttctt tacagcacta ttgggtgtga catctgactt gtgcgcagta ataatcagaa
     5641 ggggtttctt gacagtgcga aagactctcc actggcccgc atgaaagctt cgaaatattt
     5701 tcgcctcaat ttttggtcca gacatagacg atagccggca ttcgttgctt caaatgcagt
     5761 ttccaggtag ccctcgaagg acgcaggatg ccactgcttg aacaatgggt tcatgcgaaa
     5821 gcttcgacta acaacgcgaa ggtctttgaa ttctcttctt cttttttctg caagggcggc
     5881 cagaggattt ggaatcaaag aggcaaaccg ggaggttaga ggagagaaca ccgttgggtc
     5941 aagaagcgcg ccggccacaa gccttccttc catcgacgga gtcgccagaa gcaaagaagc
     6001 gccgccgatc gaatggccaa ccgcgaagaa tcgttcgtag cccaggtgtt ctgcaaatgc
     6061 agccgcctgc ctgccaaaga attcccagtt gtgaaagtct gttgtcgaat cggagccccc
     6121 gtgtcccatg aagtttagag catggacgtg aaagccgtgg tttgccaggt gatcggtcag
     6181 aggggcatag gatccagccg aaaatccatt cgcatgaaag agcagtaaag ggcgaccgct
     6241 cccctttttg atgtacttca gccgcattcc ctcaaactgg aaatccagca tattggccat
     6301 gggccagggt cgccagagaa cggtcccgga ccagaaaaaa tcgtttcccg gctcaggctc
     6361 tgctcagcgg gtaccattat tgctaatata ttaagcaaag gatccgcatg cccaatcaga
     6421 taacaaaatc cgaagcattg cgcttcatca gcgacattac gcacgccatc aaccagagcg
     6481 acaatgccga aaacgtatta gagcgtattc tttcagcatg tatccacgct acgcatgctg
     6541 aatctggaag cattatgctt atagatgagg atggcgaaaa cctgaccatc catgtttccc
     6601 aggggttggc ccccgaaagt gctcatttaa agttaagaat tggtgagggg atcactggca
     6661 atgtggctcg gactggcaag gccaggatcg tcggggacgc gacctctgag tcggactata
     6721 tccccgtcaa agaaggtttg atttc
//
LOCUS       DAORFF010000155         6717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5856_length_6717_cov_3.979285, whole genome shotgun sequence.
ACCESSION   DAORFF010000155 DAORFF010000000
VERSION     DAORFF010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5856_length_6717_cov_3.979285"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /gene="prs"
                     /locus_tag="PKK76_07935"
     CDS             complement(<1..518)
                     /gene="prs"
                     /locus_tag="PKK76_07935"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNL68440.1"
                     /translation="MILFCLGENQTLATSVCRAGQFDAGNLNSRKFPDGETYIRILDQ
                     VRNKHTAILCSLRDPDARIPSLLFLADALREQGAASVGLAAPYLCYMRQDRRFQPGEA
                     ITSRTFAKMISAGFDWLITIDPHLHRISSLSEIYSIPAVSLHAASLIADWVEKTVPEP
                     LIIGPDAESRQWA"
     gene            complement(515..2026)
                     /locus_tag="PKK76_07940"
     CDS             complement(515..2026)
                     /locus_tag="PKK76_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HNL68441.1"
                     /translation="MQGQPNQLVLRKLGIDTQQEYIVFMRSDSSVCVSEGITSLMRVQ
                     ISCRGKTIVATVNAVTGNILGPGEASLSNRAFEILGASDGDTIRVSHLSALDSMSLVR
                     AKIYGRRLEQEHYDIIIQDIVHGYYSGVHLSAFIAGCVASKLDRGEVLGLTRAMLNAG
                     QRMTWPGIVADKHCVGGIPGNRTTPIVVSIVAENGILIPKTSSRAITSPAGTADTMEV
                     MAPVNLSLPEIRRVVEKFGGCIAWGGSAKLSPADDIFIRVERALDIDSESQMIASVLS
                     KKAAVGATHVVLDIPVGETAKVRSEAEAKRLSDLFEDIGAAIGLTVQTVITDGSQPVG
                     RGIGPALEARDVLCVLHNANDAPHDLKSKSILIAGQILEQCGKAIQGQGEEMALETLT
                     SGRALSRFMNICEAQGGFREPRKAQFQHDIVSDRSGRITSVNNRKIARLAKLVGAPAD
                     PCAGIEFLAPAGTRISRGDLLFTLHADSRGQLEYALDYYARQEDFLQMSEGAP"
     gene            complement(2059..5304)
                     /locus_tag="PKK76_07945"
     CDS             complement(2059..5304)
                     /locus_tag="PKK76_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNL68442.1"
                     /translation="MVERIIRFSAKNRFLVLLMTGVLLWGAYWCAKHIPLDALPDLSD
                     TQVIIYSRWERSPDIMEDQVTYPIITGLLGAPRVKAVRGFSDFGYSFVYVIFEDGTDL
                     YWARSRVLEYLARIQPRLPEGVRAEIGPDATGLGWVYQYALTGDGSVEQHEIRAFQDW
                     NLRLHLQTVPGVAEVASLGGPVKQYQVLLDPGALLSYNISVPTITDAIRKSNRETGGR
                     VVEFSGHEYMVRGRGYVRETRDLENIVVRVDDAGTPVLLRNLGKVTLGPDIKRGASDM
                     DGEGEVTTGTVVMRSGENALAVIERAKTRLSEIQSSFPEGVKLVTLYDRSELIEKAIE
                     TLKSTLVEEIIVVSLIILLFLWHLPSATTAIITIPVSVFLAFIPLYLTGQTSNIMSLA
                     GIAISIGVLVDGAIVQVENMYHRIQIWMESGQKEPFEEVRLRALTEVGSSVFFSLLVI
                     AVSFMPIFTLVDQEGRLFKPLAYSKNFAMAIAALLALTLDPAIRMMYNRYEFFRFRPV
                     WLATLMSHLLVGRYYKEENHPVSRVLFRFYEPACNFVLKHRKATLATAVLLMISTIPI
                     YGKLGSEFMPPLNEGAILYMPVSLPGISIGEAKRSLQIQDRMLREFPEVERVFGKVGR
                     ADSATDPAPLSMVETVIVLKPESQWREVKRWYSFLPEWSKEPFRRVWRDRITYEELVQ
                     EMDQKIRIPGWTNAWTMPIKNRIDMLSTGIRTPVGIKISGSDIKEIEKIGLELETIVK
                     EVPGTRSVYAERVAGGYFLDFNLKREELARYGLTVDDALTIVESAIGGENLSTTVEGR
                     RRFPINIRYAREFRETLPDLKRILVSTPSGAQIPISYIAEVRFNSGPSMIRDEDGLLS
                     GYVYIDIAGRDIGSYVEDAKKLVNSKLKLPQGTTVRFSGQYENMLRVRERLLVVVPLT
                     LFLILVLMYANTGSAVKTAIIVLAVPFSIVGAIWLLFLLGYNISIAVWVGMIALMGLD
                     AETGVFMLLFLDLSYKEWHDSGRLQTESDLRDAIVHGAVKRVRPKIMTVACGMIALIP
                     ILWSQGTGADVMKRIAAPMVGGLATSFVLELLIYPVLFYIWKIRTTELKVG"
     gene            complement(5306..6331)
                     /locus_tag="PKK76_07950"
     CDS             complement(5306..6331)
                     /locus_tag="PKK76_07950"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNL68443.1"
                     /translation="MKTKWIVIFVFAAAVIVIIAARGKQLLTIFKYSQQAAVDPAQAA
                     ISYWTCSMHPQVHKDGPGLCPICHMQLVPVYKEDQSHQHDTGAGVVKVTEARQKQGGI
                     KLSVVKKEPFQVTLRTTGKAALDTELASAVKEFITLGKEDPALARSAAARLRILGMGP
                     EEIRQLELNPASVESLYQVATPGIAWVYATLYEQEVDLIKVGQTAIISPSYAPEKELT
                     GTVRSVSSVVDQASRSVRARILVRDPQGILRPEAYLRVTVQANLGPRIVIPRSAVVYT
                     GKRQIVFVAADADTFQAREIKVGQESGERIVIAAGLNEGDRIVTSGAFLIDSESRLQS
                     AIDEAGE"
     gene            complement(6328..>6717)
                     /locus_tag="PKK76_07955"
     CDS             complement(6328..>6717)
                     /locus_tag="PKK76_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNL68444.1"
                     /translation="VTIRVPLWTAFTNHWNILEKSGNLEAARLRLADARLKAKTDLQA
                     SLDRMDGIKKGLRLHDQSLIPGAMNAHEAARISYANGKADFSIVIQSLQVHFDHELSR
                     LEHELEFTAELLKAAESLDAFFPGKDK"
BASE COUNT         1565 a         1781 c         1662 g         1709 t
ORIGIN      
        1 gcccattgtc tgctttccgc gtccggtcca atgatcaacg gttcgggcac agttttctca
       61 acccagtccg caatcaaaga agcagcgtgc agggaaacgg caggaatgga atagatttca
      121 gaaagagaag agatcctgtg cagatgcgga tcgatggtga tcaaccagtc aaagccagcg
      181 ctgatcatct ttgcgaaggt ccgcgatgtg attgcttctc cgggctgaaa tcgtctatcc
      241 tgcctcatgt aacagaggta aggtgctgca aggccgacag atgccgcgcc ctgttctcgc
      301 agcgcatctg ctagaaacaa caaagaaggg atgcgcgcat ccggatcgcg cagggagcat
      361 agaattgctg tgtgcttatt tctgacctga tccagaatgc gaatgtaggt ttctccatcc
      421 ggaaattttc tggaattcag attccccgca tcgaactgtc ctgcgcgaca cacagacgtg
      481 gccagtgttt gattttcccc cagacagaat agaatcatgg cgcaccttcg ctcatctgta
      541 gaaagtcttc ctgtcttgcg taatagtcga gcgcatattc cagctggcct cgcgaatcag
      601 cgtgaagagt gaacaacaaa tcccccctgg agatgcgtgt tcccgcggga gcgagaaatt
      661 caatgcccgc acaagggtct gcgggcgcgc ccaccagctt tgcgaggcgc gcgatcttcc
      721 tgttgttgac actcgtgatt ctgccgcttc tatctgaaac gatgtcgtgt tgaaactgtg
      781 cctttcgcgg ttcgcgaaag ccgccttgag cttcgcaaat gttcatgaag cgagataatg
      841 ctcttccgga tgttagtgtt tccagggcca tttcttcacc ctggccctgg attgccttcc
      901 cgcattgctc tagaatttgt ccggcaatta gaatggattt cgatttaaga tcatgtggcg
      961 catcgtttgc gttgtgcaat acgcaaagta catcgcgagc ctcgagcgct ggtccaattc
     1021 ctcggcctac gggctgggac ccatccgtga ttacggtctg cactgtcagc ccaatggccg
     1081 ctccaatgtc ttcgaagaga tcgctcagtc tttttgcctc tgcttcactt cttacttttg
     1141 ctgtttctcc cacaggaata tcgagcacca catgcgttgc cccaaccgct gccttctttg
     1201 atagaacgga tgcgatcatc tggctttcgc tatctatgtc cagagcgcgt tctactcgaa
     1261 tgaatatgtc atctgccggg ctgagtttag ccgatccgcc ccaggcgatg catccgccaa
     1321 atttctcaac gactctgcga atttccggga gggacagatt cacaggagcc atgacttcca
     1381 ttgtatccgc tgttcccgcg ggagaagtaa tggctctgga agaagtcttc ggaatcagga
     1441 ttccattttc cgccacgatc gaaaccacga tgggagttgt gcgatttcct ggaattccac
     1501 ctacgcagtg tttgtcggcg acgatccccg gccacgtcat tctctgccct gcattgagca
     1561 tggcccgcgt caggcccaga acttctcctc gatcgagctt gcttgcgaca caacccgcga
     1621 tgaaggctga gaggtggact cctgaataat atccgtgaac aatatcttga atgatgatat
     1681 cgtagtgttc ctgttccagt cttctgccat agattttcgc ccgcacgagg ctcatggaat
     1741 cgagcgcact caaatggctg acgcggatgg tatctccgtc cgacgcgccg agaatttcga
     1801 acgcgcggtt ggaaagagac gcttccccgg gacccagaat gtttcccgtc actgcattga
     1861 ctgtggccac aattgtctta ccgcggcagc tgatctgcac ccgcatcaga cttgtaatcc
     1921 cttctgaaac gcagacgctc gaatcggagc gcataaaaac gatgtactcc tgttgcgtgt
     1981 cgattcccag ctttcgcaag acaagttgat tgggttgccc ttgcatcttc gccctgcctg
     2041 acctgcaagg gttccctgtc aaccgacctt gagttccgtg gtcctgatct tccagatgta
     2101 gaaaagaaca ggatagatca aaagctcaag cacgaaggaa gtagcaaggc cacccaccat
     2161 gggggcagca attcgcttca tcacatccgc gccggttccc tgagaccaca gaatgggaat
     2221 gagtgcgatc attccgcagg caactgtcat gatttttggt cgcactctct ttactgcacc
     2281 atgaacgatc gcatcgcgca gatcgctttc tgtctgcaat cttccggagt catgccattc
     2341 tttgtaagaa agatcgagaa acaatagcat gaatactcct gtttctgcat ccaggcccat
     2401 cagcgcgatc atgccaaccc agactgctat ggagatgttg tatccaagca ggaacaaaag
     2461 ccagatcgcc ccgacgatcg aaaatggaac tgcgagcacg atgattgctg ttttcactgc
     2521 cgatcccgta ttcgcataca tgagcactag aatgagaaac agtgtcagag gaaccactac
     2581 aagtagtctt tcgcgcacgc gaagcatgtt ttcatactgg ccgctgaaac gaactgtggt
     2641 cccctgaggc agttttagtt tagaatttac aagtttcttt gcgtcctcca cgtaagatcc
     2701 aatgtcgcgt cctgcaatat caatgtagac gtaaccggaa agcagcccgt cttcatcgcg
     2761 aatcatggaa ggcccggagt taaatcgaac ctctgcgata taagagattg gaatttgtgc
     2821 cccggagggc gtggagacaa ggatacgctt cagatccggc agggtttctc taaactctct
     2881 cgcatagcgg atgttaatcg gaaagcgtcg cctcccttcc acagtggtag acagattttc
     2941 tcctccaatt gcggattcta caatggtcaa agcgtcgtct accgtcaggc cgtagcgtgc
     3001 cagctcttcg cgcttcaaat tgaagtcgag aaaataccct cccgccactc tctctgcata
     3061 gacacttcgg gttccaggca cttcttttac aatggtttcc agctccagac cgatcttttc
     3121 tatttccttg atgtccgaac cagatatttt gattcccacc ggagtccgaa tgcccgtcga
     3181 aagcatatca attcggtttt tgatgggcat ggtccacgca ttcgtccagc ccgggatgcg
     3241 aatcttctga tccatttctt gaaccagttc ctcatacgtt atgcgatctc tccagacgcg
     3301 acggaacggt tccttgctcc attctggcaa aaagctatac cagcgcttca cttctctcca
     3361 ttgagattca ggtttcagaa cgattacagt ttctaccatg gatagaggag cagggtctgt
     3421 cgcagaatct gcccttccca cttttccaaa cactctttcc acttccggaa attcacgcag
     3481 catcctgtcc tgaatctgca gggatcgctt tgcttctcct atcgaaattc caggaagact
     3541 cacaggcata tagaggatgg cgccttcgtt cagtggcggc atgaattctg aacctagctt
     3601 tccataaatc ggaatggtgg agatcatcag aagcacggct gtggccagag ttgccttgcg
     3661 atgtttgaga acaaagttgc acgcaggctc atagaaacga aacaacacgc ggcttacagg
     3721 atggttttct tctttgtagt atcttccgac gagtaaatga ctcatgagtg tggccagcca
     3781 cacgggcctg aaacgaaaga attcgtagcg attgtacatc attcgaatcg caggatcgag
     3841 ggtgagtgcg agaagagcag caatcgccat tgcaaaattt ttcgagtacg caagaggttt
     3901 gaaaagcctg ccttcctggt ctacgagagt gaagatgggc atgaagctca ctgcaatgac
     3961 cagaagagag aagaagacgg atgagcctac ttccgtgaga gcacggagcc tgacttcttc
     4021 aaatggttct ttctgtccgc tttccatcca gatctggatg cgatggtaca tattctctac
     4081 ctgaacgatc gctccgtcta caagcacacc aatggaaatg gcaatgccgg caagagacat
     4141 gatgttcgaa gtctgtcccg ttaaatagag cggaataaaa gcgagaaaca cagagactgg
     4201 tatcgtaatg atggcagtcg tggcggatgg tagatgccac aagaatagta gaatgatgag
     4261 actcactacg atgatttctt ccacgagagt ggacttcaga gtctctatcg ctttttcaat
     4321 cagttcggag cgatcgtata aggtaacaag cttcactcct tcgggaaatg atgattgaat
     4381 ttctgacaga cgcgtctttg cgcgttcgat cacagcgagt gcgttttcac cggatcgcat
     4441 aacaacggtt ccagtggtta cttctccttc gccgtccata tcggatgcgc cgcgtttgat
     4501 atccggaccc agagtgactt tgcccagatt tctgagtaga acgggagttc ccgcgtcatc
     4561 gaccctcact acgatgtttt ccagatcccg cgtttcgcga acgtatccgc gcccgcgcac
     4621 catgtactcg tggccggaaa attctactac gcgaccgcct gtttcacgat tgcttttgcg
     4681 gatggcgtct gtgatggtag gaacactgat attatacgag agcagcgcgc ctggatcgag
     4741 tagaacctgg tattgcttaa ctggtccacc aaggcttgct acttcagcga cccccggtac
     4801 tgtctgcaag tgaaggcgca agttccaatc ctggaatgcg cgaatttcgt gttgttctac
     4861 ggagccatcg cccgtaaggg catactggta tacccagccc aggcccgttg cgtcggggcc
     4921 aatttccgcc ctgactcctt ctggcaatct aggttgaatt cgcgctagat attcaagcac
     4981 gcgacttcgg gcccagtaca ggtccgttcc atcttcgaag atcacataaa caaaagagta
     5041 tccaaaatca gaaaagccgc gaaccgcttt gactctgggc gcacccagga gtccagtgat
     5101 gatgggatag gtaacttgat cttccatgat gtctggactt ctttcccacc tggaatagat
     5161 aatgacttga gtgtcgctca gatccggaag agcgtctaac ggtatatgtt tcgcacacca
     5221 gtacgctccc cagaggagca ctccagtcat gaggagaacc aggaatcgat tttttgcgct
     5281 gaatcggatt attctttcta ccatcttact cacccgcctc gtcgatcgcg gattgaagtc
     5341 tgctttcgga atcaattaga aaagcgcctg acgttacgat tcgatctcct tcattcaatc
     5401 cggctgcgat aacgattcgt tctcctgact cctggccgac tttgatttct ctggcctgga
     5461 aggtatctgc gtccgccgcg acaaaaacga tttgtctttt ccctgtgtat acgactgcac
     5521 tcctgggtat cacgattcga ggacccagat ttgcctgaac tgtcacgcga agatatgcct
     5581 ctggtctgag tatcccttgc gggtcgcgca ccaaaatgcg agcgcgaaca ctacgcgatg
     5641 cctggtctac cacgctcgac acggagcgca ccgtacctgt gagttctttc tccggggcat
     5701 acgagggaga gatgatcgcg gtttgtccta ctttgatcaa gtctacttcc tgttcgtata
     5761 acgttgcata aacccaggcg atgcccggag tggccacctg atacagcgat tccacactgg
     5821 caggattcag ctccagttgt ctgatttcct ctggtcccat tcctagaatg cgcaggcgcg
     5881 ctgctgcaga gcgagcgagc gccggatctt ctttcccaag agtgatgaat tctttgacgg
     5941 cggatgcaag ttccgtatcg agcgctgctt ttccagtcgt gcgaagagtg acttggaaag
     6001 gttccttctt cacaacgctg agtttgatcc caccttgctt ttgtctggct tccgtaactt
     6061 tgaccactcc agcaccagta tcgtgttgat gcgattggtc ctctttgtaa actggcacga
     6121 gttgcatgtg acagattgga caaagaccag gaccatcctt atgcacttgc ggatgcatgg
     6181 agcaagtcca gtaggagata gccgcctggg ctggatccac ggccgcctgc tgggaatact
     6241 taaaaattgt tagtaactgc tttccgcggg ctgcgatgat tacaatgact gcagctgcga
     6301 atacgaagat aacgatccat tttgttttca tttgtctttt cctgggaaga acgcatcaag
     6361 actttctgcg gccttgagta gttcggcagt gaactcaagc tcgtgctcga gacgcgatag
     6421 ttcatgatca aaatgaactt gcagcgattg gattacgata gagaaatctg ccttgccatt
     6481 tgcgtagctg atgcgcgcgg cttcatgcgc attcatcgct cccggaatga gagactgatc
     6541 gtggaggcgc aagcccttct tgattccatc catcctgtcg agcgaagcct gcagatctgt
     6601 cttcgctttg agtcgcgcgt cagcaagcct gagtcgcgcc gcctccaggt ttcctgattt
     6661 ttcaaggata ttccagtgat ttgtgaaagc tgtccagagt ggaacgcgaa tcgtaac
//
LOCUS       DAORFF010000156         6714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5858_length_6714_cov_3.052410, whole genome shotgun sequence.
ACCESSION   DAORFF010000156 DAORFF010000000
VERSION     DAORFF010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5858_length_6714_cov_3.052410"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PKK76_07960"
     CDS             <1..555
                     /locus_tag="PKK76_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68445.1"
                     /translation="NSYLNHRTEGGNELVFLNLQGARLGLLDLERFKRQIEYCLLPNG
                     TCTDMVLQVQGRYPDTLPFDFMAPMGIWFENFALPVVINECLLQSYNGVIRLFPNWDL
                     QRRAEFHTLRAVGAFLVSAACENGAVQWVEITSEVGGTLRLIVPWAEGAQVRRGGKEF
                     VVSSGLVEMETTPGEKMQFSRNQP"
     gene            855..1643
                     /gene="trpA"
                     /locus_tag="PKK76_07965"
     CDS             855..1643
                     /gene="trpA"
                     /locus_tag="PKK76_07965"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNL68446.1"
                     /translation="MEPRIKLIERTTAGFPDMKKTKEIILAQEEAGIDVIELGIPFSD
                     PTADGPVIQAAMVRAMENADFSFERIFQCAREIHEARPDVPIVFLSYMNPVFRGFAHK
                     SDRPLEAFLAEAARAGIQGIVIPDLPADQPEANEFFRVSERFQIAQIFMSAPNTSPDR
                     LNDLRKHGRGFIYHVTSYGVTGERSELPPEVKTQMKAVRESTGLPVFAGFGFSRPDQA
                     DTVKAVADGVIVGSLHHRLIQEKGPGALEAICESTAAFVKALRG"
     gene            1687..4422
                     /locus_tag="PKK76_07970"
     CDS             1687..4422
                     /locus_tag="PKK76_07970"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL68447.1"
                     /translation="MRKIALGLLVAAAALFLLLPLVLFLSLTFNRSTVLVGSFFAETY
                     LTIIPGLATLGLALYYFERLFPQDKSKVLRRFDYISLAVFGTSAIAFVITYASSASSV
                     FHRVKSILPEPLSALGPDLYTFCLWFSFFWLIIGAIYTFQGLARAGVDRGNLGGLIIA
                     IMTSFFFALSLAKFPLFKSLENTALVNRYAFLRSPSGTLAPGGAAAYCQAPYDDKGEK
                     VVAMAPPPPGVRDDIRIVGISNETIEKVNFKWPLDWNIYADLASTLGGAENGIILFDI
                     SFVDEKGLYGGDACGVILDCRPLSADRPPRRQVDILAESFSKSKAAIISDYPLETSDQ
                     FLKPIRQNPVSSDRYNRRVEMMYAQNRLTNVKNGQLAEPGLSFPNPAVEPIGKAQKGA
                     GYANILKNEESGMNWQMPLVQRVVNLDKFRAPGYDSNRDDIFLPGIDLIVAALYYGID
                     LKKDVAVDYAAGTITLSNIPEKYKEKVNLATFENEKLDIMARPNASRTIVIPIDRRGQ
                     MHISFRGGQYCFPFQEILEVNKMTPAEGAANFKDKIVLVAMYYATGVGTAQDIHLSPY
                     GDMAGIEHHAYAINTILNQDFIYEVPPVVNLLILLAVGLIMGFYQPRVATWLAFVFAI
                     VIAAVYSVMTLFITFQVFNVLHVFPTVIILQFVQLVGFIGFRVLTEEENVKFIRNTFS
                     KFVSQDVVEELLSNPEAIALGGSKKEISVFFSDVRGFTTISEALGPEELVNLLNEYLS
                     EMTELIIEYRGTIDKYMGDAIMAFWGAPAKNDDHAYYACVASLAQYHALQSLQKRWSE
                     RNIPVLDIGIGINTGMAVVGNMGSSRRMDYTLMGDTVNLGSRLEGATKNYGVKIIISE
                     FTYERVKDRVYARELDLVRVKGKLEPVRIYELMALKNDADVEALKTSNAHKDTAA"
     gene            4422..6335
                     /gene="dxs"
                     /locus_tag="PKK76_07975"
     CDS             4422..6335
                     /gene="dxs"
                     /locus_tag="PKK76_07975"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNL68448.1"
                     /translation="MDLLSKINSPADLRKVAPEDLPRVCDEVREYLIDTLASVGGHFA
                     SNLGVVELTVALHYVLNTPEDKLIWDVGHQIYPHKILTGRREQLRSVRRRGGLSGFPK
                     REESVYDLYNTGHAGTSISQALGEAMARDRLGLNHQCVCVIGDASVASGMAFEALNHG
                     GHVQTDCIVILNDNDMSISKNVGALNQYLNRLITSPTYNKGKRIWYSILMWLPLIGPL
                     VQIFSRKIERTFKDFLTPGSLFSDFGFRYIGPVDGHNVAELVEVLEKAKELKGPVLIH
                     AYTQKGKGYVHAERDPIVYHSVSRFNREDGTMQGSKARGKISFSEIVGETLCEIIEHN
                     EKVVVITPAMIEGSGLRSVADRFPNRVFDVGIAEQHSMAFAGGLASGGAVPYLCIYST
                     FMSRAYDQLIEDVALMNFPVKLIVDRAGCVGPDGETHQGLYDLGVLLSVPNVRVYAPA
                     TGGELKAFLKFVEKDSQGPIAVRFPKDSADRESLLPANLPDVTRIRPEIHQGGKDLCI
                     LAVGIMWETALGVQKELRAHGIYATVLGIRWIRPLDIEFIQNTIHDCEYMVILEDSAI
                     NSSAASHIRWSLNSASNAKHLHTFAFPVEAIVHGTREEIMHDHGLSKEAITGALLARP
                     EFANRRIPLARQA"
     gene            6335..>6714
                     /locus_tag="PKK76_07980"
     CDS             6335..>6714
                     /locus_tag="PKK76_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNL68449.1"
                     /translation="MFSGWENLGEYLDSYTYRQSQEKFSDSIQRALADQAVLIGEAGT
                     GTGKTIAYLIPLLLYAVETETRVAVSTDTKSLQTQLLTKDIPLVESILGLSPGAELCL
                     GASNYICKRKMKAAVDQGGLLLAGS"
BASE COUNT         1664 a         1621 c         1751 g         1678 t
ORIGIN      
        1 aactcctatc tcaaccatcg caccgaaggc ggcaacgaac tagtctttct caacttacag
       61 ggcgcacgat tggggctgct cgatctggaa cgcttcaagc ggcaaatcga gtactgcctg
      121 ctgcccaacg gcacctgtac cgacatggtc ttgcaggtgc aaggacgcta tcccgacacc
      181 ttgcccttcg actttatggc cccgatgggc atctggttcg agaattttgc cctgcctgtg
      241 gtgatcaacg aatgtctgct gcaaagttac aacggtgtga ttcgtctctt cccgaactgg
      301 gatttgcagc gccgtgccga atttcatacc ttgcgggcag taggtgcttt cctagtcagc
      361 gcggcttgcg aaaacggtgc agtacagtgg gtggagatta caagtgaggt gggtggaacg
      421 ctgcggctca tcgtgccgtg ggcagagggg gcgcaagtga ggagaggagg taaagagttt
      481 gtggtgagta gtggactggt ggagatggag acgactcccg gtgagaagat gcaattctca
      541 agaaaccagc cgtagcgact gtcactggaa ggtggtcttg tcattctgta ggaatccttc
      601 gtgggtcgag ctaactattt tgtagagaag ggatccctac tctgtgctgg atcggcatga
      661 tggccagatc agctaggatt cctacggaat gacagagtat ttcacagggt ttcttggcgg
      721 aatttttcgg tataactgaa cccattactc gcaagaatga caatccacct tatgggtaaa
      781 atcgaaagct cctgattggc gacgttattt acagactaga ccatcacgca aatcatgcac
      841 accgaaagat aaaaatggaa ccgcggatta agctgattga gcggacgaca gccggatttc
      901 cggatatgaa aaaaactaaa gaaattatac ttgcacagga agaagctgga attgatgtaa
      961 tagagcttgg aattcctttt tcagacccta cggcagatgg ccccgtcatt caggcagcca
     1021 tggtgcgcgc gatggagaat gcagacttct cttttgaacg catcttccag tgcgccagag
     1081 aaatccacga ggcgcgtccg gatgtcccca tcgtatttct gagctacatg aatccggtgt
     1141 ttcgtggctt cgcacacaag agcgacagac ctcttgaagc attcctggca gaggccgccc
     1201 gcgccggaat tcagggaatt gtaattccgg acctgcctgc agaccagcca gaggcgaacg
     1261 agtttttccg agtttccgaa aggttccaga tcgcccagat cttcatgtct gcacccaata
     1321 cttctccaga tagactgaat gatttgcgca aacacggccg cggcttcatt tatcacgtca
     1381 cgagctacgg cgtcactggc gaaagatccg aactgccgcc cgaggtaaaa acgcaaatga
     1441 aagcggtccg cgaaagcacg ggacttcctg tattcgcggg ttttggtttc agtcgccctg
     1501 accaggcaga cacggttaag gcagtggcgg acggagtgat tgtgggcagc ctccaccatc
     1561 gcctgattca ggaaaaaggg ccgggtgcac tggaggcgat ctgtgagtcg acggctgcat
     1621 tcgtgaaggc tctccggggc taaaaatacg tgttgaaagg ttggcggagg tccaaaatcg
     1681 tctcctatgc gtaaaattgc cctggggctt ctggttgcgg ctgcagcact ctttctgctt
     1741 ctaccgctgg ttctttttct ttcgctcact ttcaatcggt ctacggttct ggtaggttcc
     1801 ttcttcgcgg aaacatacct gaccatcatt ccaggactgg ccacacttgg attggctctc
     1861 tactattttg agcgattgtt tcctcaggat aaatccaaag tactgagaag attcgattac
     1921 atctcacttg ctgtattcgg aacgtccgca atcgcgttcg tcatcactta cgcaagcagc
     1981 gcttcctctg tttttcatag agtaaaatcc attctacctg aaccactctc tgcactcggt
     2041 ccggatcttt atactttctg tctctggttc agtttcttct ggctgatcat tggtgctatt
     2101 tacactttcc agggtctcgc tcgcgcaggt gtggatcgcg gaaacctggg cggtttgatc
     2161 atcgccatca tgacttcgtt tttctttgca ttgtctcttg cgaagtttcc gcttttcaaa
     2221 agtcttgaaa atacagcact tgtaaaccgt tatgcgttct tgcgcagtcc aagtggcact
     2281 cttgctcctg gaggagcggc cgcctactgt caggcacctt acgatgacaa aggcgaaaag
     2341 gtagtggcca tggctccacc tccgccaggc gttcgcgacg acattcgaat tgttggtatt
     2401 tctaacgaaa cgattgaaaa agtgaatttc aaatggcctc tggactggaa catttacgca
     2461 gaccttgctt ccactctcgg aggcgcagaa aacggaatca tcctgtttga cattagcttc
     2521 gtagacgaaa aaggactcta cggcggtgac gcctgtggtg tgattctgga ttgtagaccg
     2581 ctgagtgcag atagacctcc gcgtagacag gtagacatcc ttgcggaaag tttttcaaaa
     2641 tcaaaagccg ctatcataag tgactaccct cttgaaacgt ccgaccagtt cctgaaacca
     2701 attcgtcaga accccgtttc cagtgaccga tacaatcgtc gcgtggaaat gatgtacgcc
     2761 cagaatagac tgaccaacgt aaaaaatggg cagcttgcag agcccggtct ttcctttcca
     2821 aaccccgcgg tcgaaccaat cggcaaagca cagaaaggcg cgggctacgc gaacattttg
     2881 aagaacgaag aatccggtat gaactggcag atgcctctgg ttcagagagt ggtgaatctg
     2941 gataagtttc gcgcgccggg ctacgactcc aatcgcgacg acatcttcct tcctggcatt
     3001 gacttgattg tcgcggctct ttattatggg attgatttga agaaagatgt ggcagtagac
     3061 tatgcagctg gcaccatcac tctaagcaat attccagaga aatacaaaga gaaagtaaat
     3121 ctggcaacgt ttgaaaacga gaagctggac atcatggcgc gcccgaatgc atcgcgcacg
     3181 attgtaattc caatcgacag acgcggacag atgcacatca gtttccgcgg cggacagtac
     3241 tgctttcctt tccaggaaat tcttgaagta aacaagatga ctccggcaga gggagccgcg
     3301 aacttcaaag acaagatcgt acttgtggcc atgtactacg ccaccggtgt aggaacagcg
     3361 caggatattc acctttcccc ttacggtgac atggcgggca ttgagcacca cgcatacgcc
     3421 atcaacacga ttctaaacca ggatttcatc tacgaagttc cgcctgtcgt gaatcttctg
     3481 attcttcttg cggtaggctt gatcatgggc ttttaccagc ctcgagtggc cacatggctc
     3541 gcgtttgttt ttgccatcgt aattgcggct gtttattcgg taatgactct gtttatcacc
     3601 ttccaggtgt tcaacgttct gcacgtgttt ccgaccgtga tcattttgca gtttgtgcaa
     3661 ctggttggtt tcattggatt tagggtgctg acggaagaag aaaacgtgaa attcattcga
     3721 aacacattct ccaagttcgt atcgcaggat gttgtggaag aacttctctc gaaccctgag
     3781 gcgatcgcac tgggtggatc caagaaagaa atcagcgtgt ttttctcaga cgttcgcggg
     3841 tttacaacga tctcagaagc acttggtcca gaagaactcg tgaatctgct caatgaatac
     3901 ttgagcgaaa tgacagagct catcattgaa tatcgcggca ccattgataa gtacatggga
     3961 gatgcgatca tggcattctg gggcgctcct gcgaaaaatg acgatcatgc ctattacgca
     4021 tgcgttgcat ccctggcgca gtatcatgct ctccaatctc tgcagaagag atggtcggag
     4081 cgaaacattc cggttctgga cattggaatt ggaatcaaca caggtatggc ggtcgtaggg
     4141 aacatgggct cgagccgtcg tatggactac actctaatgg gagacactgt gaacctggga
     4201 tcgcgccttg agggtgcgac caagaactat ggtgtaaaaa tcattatttc tgaattcaca
     4261 tacgaacgag tgaaggatcg cgtatatgcg cgtgagctgg atcttgtgcg ggtgaaggga
     4321 aagctggaac ctgttcgcat ctatgaactg atggcactca agaacgatgc agatgtagag
     4381 gctctgaaga cttcgaacgc acataaggac acagccgcct gatggatctt ctttcgaaga
     4441 tcaatagtcc ggcggatctg agaaaggtcg cgccggagga cttgccccgt gtttgcgatg
     4501 aagtgcgaga gtatctcatc gatactcttg cgtccgttgg tgggcatttt gcttccaatc
     4561 tgggagtggt ggaactgacg gttgccctgc attacgttct caatacccct gaagacaaac
     4621 tgatctggga tgttgggcat cagatttatc cgcataagat actaaccgga aggcgagaac
     4681 aacttcgcag cgtgcgccgt cgcggaggtc tatctggttt tcccaaaaga gaagaatccg
     4741 tctacgattt gtacaacaca ggacatgcgg gtacttccat ttcgcaggca ctcggagagg
     4801 ccatggcccg cgatagactg gggctgaatc atcagtgtgt gtgcgtgatt ggagatgcat
     4861 cggtggccag cggcatggca ttcgaggcgc tcaaccatgg cggacatgtg cagacggact
     4921 gcattgtcat tctcaatgac aatgacatgt ctatatccaa gaatgtgggc gcactcaacc
     4981 agtatctcaa tcgtctcata acatctccca cttacaacaa gggaaagcgc atctggtatt
     5041 ctatcctgat gtggctgcct ttgatcggac cgctcgttca aatcttttcg cgcaagatag
     5101 aaagaacgtt caaagacttc ttaactccag gcagcctatt ttctgatttt ggatttcgct
     5161 acattggacc tgtggacgga cacaatgttg cagaacttgt agaagtgctc gagaaagcaa
     5221 aggagctcaa gggccctgtt ctaattcacg cctatactca aaaaggaaag ggatacgttc
     5281 acgcggagag agatccaatc gtatatcata gcgtatctag attcaatcgc gaagacggaa
     5341 ccatgcaggg ttcgaaagcc cgtggtaaaa tttcattttc cgagatcgtt ggtgagacgt
     5401 tatgcgaaat cattgaacac aatgagaaag tagtggtgat cactccagcc atgattgaag
     5461 gctccggcct cagatctgtt gcggatcgtt tcccgaatcg cgtatttgat gtgggaattg
     5521 cagagcaaca ttccatggct tttgccggag ggctcgcctc gggtggagcc gttccctatc
     5581 tatgcattta ttctacattc atgagtcgcg cctatgatca gttgattgag gatgtggctc
     5641 tcatgaattt cccggtgaag ttgattgtgg atagagcggg ttgtgttgga ccggacggag
     5701 aaacgcacca gggtctgtat gatcttggag tgcttctcag tgtgccaaat gtgcgtgtgt
     5761 atgcgccggc tacgggcgga gaactcaaag cattcttgaa atttgtagaa aaagattccc
     5821 agggacccat cgccgttcga ttccccaagg actcagcaga tcgggagtct ttgcttcctg
     5881 ccaatctccc ggacgtgact cgaattcgcc ctgaaatcca tcagggagga aaggatcttt
     5941 gtattctggc agttggcatt atgtgggaga cagctcttgg agttcagaag gaactgagag
     6001 ctcacggaat ctatgcgact gtgctaggca ttcgctggat tcgtcctctg gacatagagt
     6061 tcattcaaaa tacaattcac gattgcgaat acatggtcat cctggaagac tctgccatca
     6121 acagcagcgc tgcatctcac attaggtgga gtctgaattc tgcgagcaat gctaagcatt
     6181 tgcatacttt tgcatttcca gtggaagcaa ttgttcacgg cactcgtgaa gagatcatgc
     6241 acgatcacgg cctgagcaaa gaagcgatca caggtgcctt gcttgcgcgt cccgaattcg
     6301 caaatcgcag gatacctcta gcaaggcagg cctgatgttc agtggctggg agaatctcgg
     6361 cgaatacctg gacagctaca cctatcgcca gtctcaggaa aaattttcag actccatcca
     6421 gcgtgccctc gcagatcaag cggtgttgat cggcgaagca gggaccggca caggcaagac
     6481 gatcgcctat ctcattcctc ttttgctcta tgctgtggaa acggaaactc gtgttgccgt
     6541 atccacggac acaaaatcac tccagacaca gctcctaaca aaggacatac ctcttgtaga
     6601 aagcatactt ggcctatctc ccggagctga gctttgcctt ggcgcgtcca actatatctg
     6661 taaaagaaaa atgaaggctg cagtggatca gggcggtctg cttcttgcgg gctc
//
LOCUS       DAORFF010000157         6685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5899_length_6685_cov_4.083710, whole genome shotgun sequence.
ACCESSION   DAORFF010000157 DAORFF010000000
VERSION     DAORFF010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5899_length_6685_cov_4.083710"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="PKK76_07985"
     CDS             <1..1013
                     /locus_tag="PKK76_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL68450.1"
                     /translation="ANYMRIVAGNARHLLQLVNEVLDYSKLETGAMQFEKVDFRLRDM
                     ILGVIDSESIKAASRGDIVEIEIDAAVPSIVRGDPTRIAQVLYNLVNNAVKFTDNGVI
                     RVEIKALSQDAQTVDLQFSITDTGIGIAQEQRSIIFDRFAQADSGIARKYGGTGLGLA
                     IVQMILQALGSSLQLESSPGNGSRFYFHLKFEKGGEAATILEAPVSNERLAGIRVLVV
                     DDNEINRMLAEKLLQRWGATVDGAADGEEGVRKAQSSAYDVILMDLQMPGMDGYEAAR
                     RIRDLGIKTPLIALSAAALDEVKDRALEAGMDDFVSKPFHQEELYSRLVQHTSPDVRK
                     NH"
     gene            complement(987..2450)
                     /locus_tag="PKK76_07990"
     CDS             complement(987..2450)
                     /locus_tag="PKK76_07990"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL68451.1"
                     /translation="MIESVSEDRVRDLKFEVDLFLAKLQLINEKGTGQPSVDKKRIKE
                     NLHEFLRDDMIYFLQKLLSEHRTTFEKMMPGLVPSHREIIFQLKELQARVFYPVGKDF
                     SKTSMIGRWLLALHARGHLSPHKVMNYFNIYKNRLYSLLEEKPFGFSDTETLVRRMLF
                     STDYEVSFDEIYSAEIELKREITFTLLRRVSTQSRIDYLVSKEVVEELSDNFIRILHR
                     ETAARLKQALAEMDELNKRILAEKKTVESEIKIAAKSQDKNLQKSLPADDPRIKFALW
                     YDPLMSVGGDYYNVIRVSEHEYAIFLADISGHGIGAAMFFNTLRLAFEESLESADRPE
                     KTMKLVNESVYGKLSDNFVTAIYVYVNLKKKIIKYCNAGHPKAFLFQKDGDRMRVRFL
                     RQTGRVLGLFRKVSYKEKQIPLGGTARLIVYTDGITEAYGRENQMLGERGFLSMFRHT
                     LTSGAEDAMESIRKDVLAFSGTEQREDDRSVILADIG"
     gene            2556..3380
                     /gene="uppP"
                     /locus_tag="PKK76_07995"
     CDS             2556..3380
                     /gene="uppP"
                     /locus_tag="PKK76_07995"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HNL68452.1"
                     /translation="MIQVTEAAILGIIQGLTEFIPISSTAHLRIIPAMLGMGDPGAAY
                     TAVIQLGTLFSLIIYFWRDLLNFAQSSLQGLASGSPFRDENARMVWYLGVGTIPVCVF
                     GLLFAHKIKGDFRSLYVIAGSLIVFAITLYIADRVSRRHKTIHQFDLRDALLIGLAQS
                     VALIPGASRSGMTLTMALLLGYTRESGMRISFLLSIPAIAISGLYELYEERRALAEAG
                     FVGLAVATIVSGIVGYMSVAFLLRYLKTHSTTAFVLYRIAMGVVILGLLRAGYLMS"
     gene            3478..6090
                     /locus_tag="PKK76_08000"
     CDS             3478..6090
                     /locus_tag="PKK76_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtN system VIT domain-containing protein"
                     /protein_id="HNL68453.1"
                     /translation="MSENRIQKYFIRPVRIVGMELPAWGWMVGSFAAICFLAVCVYAL
                     LYANVLRWLNSSILLWGSGVFLGIVFLGLFLVLLRAVFSSIRQFTIFTLVLAAASFAT
                     NAYVLSHEVFPPFTSRTEVALWVCFIALAISALNEWLSDPIRYALLPLLSAGAFLCVA
                     FVIVLGPLMAFAWFFVWAAGLGFLPYSPLFALVAFVLNLMRLESSVRQRVVARWLIAG
                     PAALIVIYSIYYKIQWNRAEEVMRRPEISAGHNRLDEDLPLWIQKAARIPANHVTQMV
                     LQPNRRSEISIFGLQTLFDPLAFLSSTSPWSGSRGVDIISAEDSGRMLHLLYGQSHAY
                     LDRLWRGHSLITTNVQTHVQIHPELRVAYTETTLSIFNEDQDSNHKVMGLRLSAGNVP
                     QEAIYTISVPEGSICTKLSLWVNGEERPARLTFKSTARRAYETIVSVERRDPSYVEWL
                     DGNRLRLRVFPVSPGQYRTVRVGIVSPLRADEGRLTYNRIEFDGPVQTFAEQSVLVDL
                     FSSARLDVESSGMFLKEKVVSDGQVRQFRGSFVRPHWSFSFPAPAAGGSISAGGKSFL
                     VHPLQMENKPFSPQAILVAVNGSRSRAAWKEFISKTYGLGLPVYILTNEWFETADVKK
                     AMAYLDDCEIPAFNLYPPAFPAKRKLGPVLWVTSGEEQSIPLGELRGSIRFANTQIAA
                     TERPADAVAVLNGRVSEYTASLIDLDRLLLVAQDEASTLKMLGDRRIQVPRETDRVFP
                     LRASAIELEESAESASRPGSDLLVRMLMYRRIMRTLGQRFFNHDLDNADMVKLARDGM
                     IVSPVSTLIVLESEADYKRFGIENDPSLLGQSKLEAPGAVPEPHELALLLLILLVIFY
                     TRTRIRTAVHAHSR"
     gene            6074..>6685
                     /locus_tag="PKK76_08005"
     CDS             6074..>6685
                     /locus_tag="PKK76_08005"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HNL68454.1"
                     /translation="MRIRDESQSVSVMKMRGESRLDRLMQGHPQTWLTITAILSLVPF
                     LGTAYALFGGAPQSYLSLYPIGFLILFRSQKVRHRWLPLLFVALSILITLSGILTNRL
                     SMIWVGSFWSFLGVCNLYGVPFAFPAPLLVFAAPPLAAFTSLLGGFSLRLWLTARAVQ
                     ILSLLDVTATAQGNVIQFRGNQFSVDRVCEGMKMAVATALIAMV"
BASE COUNT         1457 a         1619 c         1724 g         1885 t
ORIGIN      
        1 ccgcaaacta tatgcgaatt gtagcgggga atgctcgtca cctgttgcag ctcgtaaatg
       61 aagttctgga ctacagcaaa ctcgaaacgg gcgccatgca atttgaaaaa gtggatttca
      121 gattgcgaga catgattctt ggtgtgattg attctgaatc catcaaggcc gcatcgcgcg
      181 gagacattgt ggaaattgag attgatgctg ctgtgccttc catcgtacgc ggagatccta
      241 ctcgcattgc acaggttctt tacaatctcg tgaacaatgc agtgaagttc acggacaatg
      301 gagtcattcg agtagagatc aaggcgctct cgcaggatgc gcagactgtg gatcttcaat
      361 tctccattac agacactgga attggaatcg cacaggaaca gcgatcgatt atttttgatc
      421 gattcgctca ggctgattct ggaatcgcgc gaaagtacgg cggaactgga ctggggcttg
      481 cgattgtgca gatgatcctg caggcactcg gctcgtcttt gcagctggag agttctcccg
      541 gaaatggaag tcgcttttac tttcatttga agttcgagaa aggcggagaa gccgccacaa
      601 tcctcgaagc tcctgtgtca aacgaacggc tggcgggcat tcgggttctc gtggtcgatg
      661 acaacgaaat caatcgcatg cttgctgaga aactgctgca gagatggggt gccactgtgg
      721 acggcgccgc tgacggagaa gagggagttc gaaaggcgca gagcagtgct tacgatgtca
      781 tcttgatgga tctacaaatg ccgggaatgg atggatacga ggccgcgcga aggatccgtg
      841 atttgggaat caagacgcct ttgattgctc tttctgccgc agcactggac gaggtgaagg
      901 atcgtgctct cgaagcggga atggacgatt ttgtatccaa gccttttcac caggaagagt
      961 tatactctcg cctggtgcag cacacttcac ccgatgtccg caagaatcac tgaccgatcg
     1021 tcttcgcgtt gttctgtgcc agaaaacgcg agcacatcct tgcgaatgga ttccattgcg
     1081 tcttctgcgc cgctggtcaa tgtgtggcgg aacatggaca aaaatcccct ttctccgagc
     1141 atctggtttt cgcgtccgta tgcttccgtg attccgtctg tgtagacgat caatctggca
     1201 gttccaccca gtgggatttg tttttctttg tagcttactt tgcggaacaa tccaagaaca
     1261 cgcccggtct gtctgagaaa acgcactcgc attctatctc cgtctttctg gaacagaaat
     1321 gctttaggat gtcccgcgtt gcagtatttg atgattttct ttttcagatt cacatacacg
     1381 tagatggccg tgacaaagtt gtccgagagt tttccataca cagattcatt cacgagtttc
     1441 attgtctttt ctggtctgtc cgcagactcg aggctttcct caaacgcgag gcgaagtgta
     1501 ttgaagaaca tggcagctcc aatgccgtga cctgaaatat ccgcaagaaa gatagcatac
     1561 tcgtgttccg aaacgcgaat cacgttgtag tagtctccac caacactcat gagcgggtca
     1621 taccagagag caaacttgat gcgcggatca tctgccggca gactcttctg gagattcttg
     1681 tcttgagact tggcggcgat tttgatttct gattctaccg ttttcttttc cgcaagaatc
     1741 cgcttattga gttcatccat ttccgcgaga gcctgtttca gtctcgcggc agtttccctg
     1801 tgcagaatgc gaatgaagtt gtctgaaagt tcctctacta cttctttgct caccaggtag
     1861 tcaatgcggc tttgcgtgga gacacggcgc agaagagtaa atgtgatttc tctttttaat
     1921 tctatttccg cagaatagat ttcatcaaac gaaacttcgt agtcggtgga gaaaagcatt
     1981 ctgcgaacca gggtttccgt atctgaaaac ccgaaaggtt tttcttcgag gagcgaatag
     2041 aggcgattct tataaatatt gaagtaattc atcaccttgt gagggctcag atggccgcga
     2101 gcatggaggg ccagaagcca tctaccaatc atggaggttt tgctgaagtc tttgcccact
     2161 ggatagaaaa cgcgagcctg tagctctttg agctgaaaaa taatttcgcg atgcgaagga
     2221 acaagaccgg gcatcatctt ttcgaacgtt gtgcgatgct ccgaaagtag cttctgcaag
     2281 aagtagatca tgtcgtctcg cagaaactcg tgcaggtttt ccttgattct cttcttgtct
     2341 acggacggct gtccggttcc tttttcgttt atgagctgga gtttggccag gaaaagatct
     2401 acttcgaatt tcagatctcg cacccgatcc tctgaaaccg actcgatcat tcggtccact
     2461 tgctcctgca gtctttgttt ttcggggacg tccactctat gaccagaaaa cagtaaatgg
     2521 tttgattgga aagtaatacc tgggcccatg acactatgat tcaggtcaca gaggcagcca
     2581 tcctgggcat cattcaaggg ctcacggaat tcattcctat ttccagtacc gcacatttac
     2641 gcatcatccc ggccatgctc gggatggggg acccgggggc cgcttacacc gctgtgattc
     2701 agctggggac tcttttctca ctcatcattt atttctggcg cgaccttctg aatttcgcgc
     2761 aatcgtcgct gcagggtctt gcttccggat ctccatttag agacgaaaat gctcgcatgg
     2821 tttggtatct gggcgttgga actattccag tatgcgtgtt cggtttgtta ttcgcgcata
     2881 aaataaaggg cgactttcgc tcactgtacg tcatcgcggg gagtctgatc gtttttgcga
     2941 tcactctgta tatcgcagat cgcgtttcgc gtcgtcataa aaccattcat caatttgatc
     3001 tgcgcgatgc attgttgatt ggactggcgc agagcgttgc attgattcca ggcgcttctc
     3061 gttcaggcat gactctgact atggctctct tgctcgggta tacacgtgaa tccggcatgc
     3121 ggatttcttt cctgctcagc attcctgcca ttgcgatttc cggtttgtat gagctgtatg
     3181 aagaacgccg tgcactcgcg gaggccggtt ttgtgggtct ggctgtggcc accatcgtgt
     3241 cggggatcgt tggatacatg agcgttgcct tcttgctgcg ctacctgaag acgcattcta
     3301 ccactgcatt tgttctgtat cgcattgcca tgggagtcgt gatcttaggg cttttgcgtg
     3361 ccgggtattt gatgtcctga gggcttttct caaaaaaggc cccttgctca cacaattcac
     3421 ataattcgca caaattcttc ccatatccaa cgcctacctt gattgaggag ttgcgatatg
     3481 agtgaaaaca ggattcagaa atattttatc cggccggtgc gaattgttgg aatggagctg
     3541 ccggcctggg gttggatggt cggatcattt gccgccatct gcttcctcgc tgtctgtgtg
     3601 tatgcgttgc tctacgccaa cgtgttgcgg tggctaaatt cttccatctt actctggggt
     3661 tcaggagtct ttctcggaat cgtttttctg ggattgtttc tagttctact tcgcgccgtg
     3721 ttttcttcga ttcggcaatt cacgatcttc accctggtcc tggcagctgc atcctttgct
     3781 actaacgcgt acgtcctgtc acatgaagta ttccctccat ttacatccag aacggaggta
     3841 gccctctggg tttgtttcat cgcacttgcg atttcagcac tgaacgagtg gttaagcgat
     3901 ccgattcgat atgcattgtt acctcttctg agtgcaggcg cttttctctg cgtcgcgttt
     3961 gtcattgtgc ttggaccgct gatggctttt gcgtggttct ttgtctgggc ggcaggcctc
     4021 gggtttcttc cttatagtcc gcttttcgcg ctcgttgctt tcgttttgaa tctgatgcgg
     4081 cttgagtctt ctgtgcggca gagggtagtc gcgcgctggc tgatcgccgg gccggccgcc
     4141 ttgattgtaa tctactccat atactataag atccagtgga atcgcgcaga ggaggtgatg
     4201 cgaagaccag agatctctgc gggccacaat cgcctcgatg aggatcttcc actctggatt
     4261 cagaaggccg cgcgaattcc tgccaaccat gtaacacaga tggttctaca accaaatcga
     4321 agatctgaaa tttccatttt tggtctgcag actctgtttg atcctctcgc atttctcagt
     4381 tctacctctc cctggagtgg gtctcgcggc gtggatatta tttctgcaga agattcaggc
     4441 agaatgctgc acctgttgta tggacaaagt cacgcctatc tggacagact gtggagaggg
     4501 cattcgctca tcactacgaa tgttcagact cacgtgcaga ttcacccaga gcttcgcgtt
     4561 gcttatacgg aaactactct ttctattttc aacgaagatc aagattcgaa tcacaaagtc
     4621 atgggtttgc ggctcagtgc gggcaatgtt cctcaagagg ccatttacac aatttctgtt
     4681 ccagaaggaa gcatttgcac aaaactatct ctgtgggtga acggggaaga acgcccggcg
     4741 agacttacct tcaaatctac cgcaagacgc gcgtatgaaa cgatagtgag cgtggaaagg
     4801 cgcgatccgt cttacgtgga atggctggac gggaatcgtc ttcgcctgcg agtgtttccg
     4861 gtctctccag gacaatatag aaccgtgcga gttggaatcg tctctcctct tcgcgccgat
     4921 gaaggtcgcc tgacgtacaa tagaattgag tttgatggtc ccgttcaaac atttgcggaa
     4981 cagtcagtgc tcgtggattt gttttcctcc gctcgcctgg atgttgagtc atccggaatg
     5041 tttctgaaag agaaagtggt ctcagacgga caggtcagac aatttcgggg atcgtttgtt
     5101 cgtccgcact ggtccttttc ttttccggca cctgcagcag gtggttccat ttccgcaggc
     5161 ggtaagagct ttctggtgca cccgctccag atggagaata aaccgttttc tccgcaggcc
     5221 attttagtcg cggtgaacgg ttctcgttcg cgagccgcct ggaaggaatt cataagtaaa
     5281 acatacggat tgggtctgcc tgtttatatt ctgacgaatg aatggtttga gactgcggac
     5341 gtcaaaaagg caatggcata cctggatgat tgcgaaattc ccgcattcaa tctgtatcct
     5401 ccagcctttc ccgccaagcg aaagctcggg cccgttttgt gggtcacctc cggagaggag
     5461 caatccattc ctctaggtga attgcgagga tccattcgat tcgcgaacac gcaaatagct
     5521 gcaacagaga ggccggcgga tgccgtggca gttttgaacg gtcgcgtttc tgagtacacg
     5581 gcgagtttga ttgacctgga ccgtctgctt ctggtggcgc aggacgaagc gtctactttg
     5641 aaaatgcttg gcgatcgtag aattcaggtg cctcgcgaaa cagatcgcgt attcccactc
     5701 cgggcatctg cgattgaact cgaagaatca gccgagtcgg cttcgagacc tggctcagat
     5761 ttgctggttc ggatgctgat gtacaggcga atcatgcgaa ctctgggtca gagatttttc
     5821 aatcacgatc tggacaatgc agatatggtg aaactggcgc gcgatgggat gattgtatct
     5881 cctgtttcta cgctgatagt actcgaatca gaagcggact ataagcgatt cggaatcgag
     5941 aatgatccgt ctcttttagg ccagagcaaa ctcgaagctc ccggagcagt gcctgaacct
     6001 cacgaactag cacttctatt gctcattctg ctggtgattt tctacactag gacgcgcata
     6061 cgaacagccg tgcatgcgca ttcgcgatga atcgcaaagt gtgagtgtca tgaagatgcg
     6121 cggcgaaagc agactggatc gtctgatgca aggtcatcca caaacctggt tgactatcac
     6181 agccattctc agcctcgttc cattcctcgg taccgcctat gctttgttcg gcggggctcc
     6241 tcaatcctat ctgagtttat atccaatagg atttttgatt ctttttcgca gtcagaaagt
     6301 tcgtcacagg tggctgcctt tgttgtttgt agcactgagc attctgatca cgttaagcgg
     6361 cattctaacg aatagattga gtatgatctg ggtcggatcc ttctggagtt ttctaggagt
     6421 ctgcaatctc tatggggttc cgtttgcttt tccggctcct cttctggttt ttgctgctcc
     6481 tcctcttgcc gccttcacat ctttattggg cggattctct ttgaggctgt ggctgactgc
     6541 ccgggccgtt caaattctct ctttgctcga tgtcacagcc acagcacagg gaaatgtcat
     6601 tcaatttcgc ggtaaccagt tcagcgtcga tcgagtgtgc gaaggaatga agatggccgt
     6661 cgccactgct ttgattgcca tggtc
//
LOCUS       DAORFF010000158         6682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5903_length_6682_cov_4.380564, whole genome shotgun sequence.
ACCESSION   DAORFF010000158 DAORFF010000000
VERSION     DAORFF010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5903_length_6682_cov_4.380564"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="PKK76_08010"
     CDS             <1..940
                     /locus_tag="PKK76_08010"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNL68455.1"
                     /translation="GTVLTTEYIEGINISEIEELDARGIARREAAELLVETYVRMIFD
                     DRFYHADPHSGNIFVIPPDGVRPLRIGLVDFGATQSIPERMFNVFERVFEILRRRDIP
                     AFVDLALETGMLSPDADAEVYANLAEVMYARYGSFKIENFYKINPVRFGRMIKMRDLN
                     AVGMRLRDIISEVRLPRRYIYFSRAVALILSIAIRLDDRVNVFMTAMPHLERYLRARQ
                     RSPLWYMRSKNWQSALKNALTDKRLEVFRPLHHPREPEKKENRTAREAMLASLCALSG
                     FAALQLQQTGHPETAVLVLPASVIFGLIFLYKVMKD"
     gene            956..3565
                     /locus_tag="PKK76_08015"
     CDS             956..3565
                     /locus_tag="PKK76_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68456.1"
                     /translation="MNSGEFEHHLRTIANDDRDFLYPNWIASAQYFERLARSGESIFP
                     ALDPLLRIFYETHQVPVMQPAIRAVMIELLRENRTHEIENTFLHDDFKTWVIEALADL
                     ARSFDIGPAMPSIVLHFEASPFRAAEAIAMQIRKKRATVAQVCDLMMTEEFSRKSLVR
                     VLATTASSGIDITEHVSSYARLLTYQDSQKLAIQELPWFTGESKANIAIVEELGRIAN
                     DPNAESDVRERAAYFLADHYSTTDAEKFSSLLENESSFVRAGTAIFLAHTIPKSRGTR
                     RAEWIDKLTLYLLDQDSYVRSVALRQIDSMIQSGNKFEPSLDAIAKLAQAAKKQEDLI
                     PFLLHFASESKSLKSVIEKSLASPQSENAALILKALREPTVRRCSICSSLNRVESWNH
                     ELRTPAQIKKMRESGNLRFCTECENIYQFSYSSEVEDNHTSESFELERLHPAAALKML
                     QGEDAIQYKEQLPRLLQSLRSETSHVDEWIRSEAAWKLAEYLLSTEQWTEIDTLSLQN
                     QTCLKEITLAVASAVRSGVDAGHTLTTIRRAMQAEHCETRMNSAFVLSCMSLQGESNE
                     LPEILYSTDSCVLQGCLQALFAHASRDLPCPHLDRIAALTLHDDDRVRAFANYVVERA
                     KENGQKIPFLVPNLMKALRSKNARMKQDGMRFLRSEITQQNAAQILGEAVSLLKDSER
                     YGLVELIEACAEAGASLERYTGQFISDLQDSRGDLLRLLTVAARQNPSMIGRNATDAI
                     AECLNDSRLLPAASHLFEFLSNSKCDLTTAMPAIQSFIDRAVSTDSYTESLIQVLIRS
                     LAGLSDYESIMKLLSHRTTTIRLSVLYRIKDLASEGLQFRAILEPLQSLLKDPSAGVR
                     EMAEKVITQVSQS"
     gene            3606..4883
                     /locus_tag="PKK76_08020"
     CDS             3606..4883
                     /locus_tag="PKK76_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNL68457.1"
                     /translation="MKRFFIPPDFNLAAAWFADATLPASLRTYQNIHELEITPADREM
                     LRSLRHNRLLFFTNHPSQAEPMIAYHIANVMGSRFHYMATRRAFDFLFGAVGKLFQSV
                     GAYSILPGVADRDSMRMTRQILSAPGGKLAIFPEGEPMCGENDSLMPFQPGFLKLGFA
                     ALQDARKTEPTANISVLPGFIKYVIKSSREQIIEHLTQSIGTIEKKLGLEAGHRNLLR
                     QFLMVGRVLLENSEKEYGITPPADADFDFRIGKLRHTILDRVAEMMKLKHYNTGADAI
                     HKLRHLTSIIELLEVGYESPELPVLTPSELAAANRECIKAYDFIVIKRDYLVSRPTPE
                     RFYEWLQRFESLALGRTPRALGGEPHPLPRRAVITFAKPFTLGEHYDAFQKGKGEYVE
                     QLTNEIRAQMQALLDASASLTYTIVDPGDVGGV"
     gene            complement(4844..>6682)
                     /locus_tag="PKK76_08025"
     CDS             complement(4844..>6682)
                     /locus_tag="PKK76_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL68458.1"
                     /translation="EPGDMAQLEELQRGRQLIVYALVFFAVMFVAEAIAGFGFGRWFS
                     HRLESERKSEFSRAFDNYLHQRWNFHSGAVTGYAWWAEPWNLARTGRWAEAKALFEGD
                     PTLQGSFDYFAVYSQSDTPRFEFPGAGNTPLVIPPDIVRFLVESRSMQRGETNLILRI
                     GDKPYLVTAAAMADAQGRPQIPGICVFAFSLDRFLISAREILPATLELSEDGSGFLSV
                     PLVASMRSTDAPLYVRITPNTLMEDLIRNILLAFLAFQIVLSILLFAVISPRYVRERT
                     MRLEKMVRASRILNRELELRVDELTAARTEIQKSEKKYRDLVESSREIIFSIDSKGNI
                     TTINQAVSSLLGFQPESLIGKSILDLIYVSPDRSMNFRREFISEQIESVIQNQKVASF
                     KTEFVTRYNEPRELETTLEYVPLDGGYAIFGKAASMVEDMLATYCERESMKFVIGNYL
                     TLSEQISQRVTNNLGLHCDAGTTLQIRICVSEMIINGIEHGNLGITYEEKTNATEDGS
                     YLALLRRRQFEAPYSSRKLKVVYSLNPRRLLIVVQDEGAGFNHTQILKDNSMEANKKM
                     HLHGRGITMAKSVFDSMRYNEKGNRVALLKRFRNQTPPTSPGSTIV"
BASE COUNT         1788 a         1728 c         1577 g         1589 t
ORIGIN      
        1 cggcacggtc ttaaccacag aatacattga aggcataaac atttctgaaa tagaggagct
       61 cgatgcgcgc ggcattgcca ggcgcgaagc agcagagctt ttagtagaga cgtatgtaag
      121 aatgatcttc gatgatcgtt tctaccacgc agacccgcat tcgggaaata tttttgtaat
      181 tccgcctgac ggtgtacgtc ctctgcgaat tggcctggta gactttggcg ccacacaatc
      241 gattccagaa agaatgttca acgtattcga aagagtcttc gaaatactga gaagacgcga
      301 tattcccgcc tttgtggatc tggcgctaga aactggaatg ctgtctccgg atgcggacgc
      361 ggaagtgtat gccaaccttg cagaagtgat gtatgctcga tacggatcct ttaagataga
      421 gaacttctat aaaatcaatc cggtgcgatt tggtcgaatg atcaagatgc gcgacctcaa
      481 cgcggtcggc atgcgcttgc gagacatcat ttcagaagtg cgtcttccgc gacgttacat
      541 ctactttagc cgggctgtgg cgctcattct ttcgatcgcg attcgcctgg atgacagagt
      601 caacgtattc atgactgcca tgccacatct ggaaagatac cttcgcgcca ggcaacggag
      661 tcctctctgg tacatgcgaa gcaagaactg gcagtccgct ctcaagaatg cactgacaga
      721 taaaagactg gaagtctttc gaccgcttca tcatcccagg gaaccagaga aaaaagaaaa
      781 tcgcaccgcg agagaagcaa tgctcgccag tctatgtgca ctgtcaggat ttgccgccct
      841 tcagttgcaa cagacaggtc atccagaaac ggctgtcctg gtgctgcctg catccgttat
      901 atttggattg atcttcctct ataaagtaat gaaagactaa catttttgca gggaaatgaa
      961 ctccggcgaa ttcgaacatc acctgcgaac catcgcaaac gatgatcggg actttttata
     1021 tccaaactgg attgcgtccg cccaatattt tgaacgcctg gctcgaagtg gagagtcgat
     1081 ttttccagca ctcgaccctc tgctacgaat cttttacgaa actcatcaag tcccggtgat
     1141 gcagcccgcc attcgagcgg tgatgattga acttctcaga gaaaaccgaa cccatgaaat
     1201 cgaaaacact ttccttcacg acgatttcaa aacctgggtg atagaagcac tcgcggatct
     1261 ggccaggtct tttgacatcg gacctgcaat gccgtctatc gttcttcact ttgaagcttc
     1321 tcctttcaga gcagccgaag ccatcgcaat gcagataagg aagaagaggg caaccgtggc
     1381 tcaggtctgt gacctgatga tgaccgaaga gttttcgcgc aagagcctgg tccgcgtttt
     1441 agctactacc gcctcctccg gaatcgacat aacggaacac gtttctagct acgctcgctt
     1501 gcttacttat caggattcgc agaaactcgc gatccaggaa cttccctggt tcacagggga
     1561 atcaaaggcg aatattgcga tcgtcgaaga gctcgggcgc attgcaaatg atcccaatgc
     1621 agagtcagat gtacgggagc gcgccgcata ctttttggcg gatcactact ccaccacgga
     1681 tgcggaaaaa ttctcctcct tacttgagaa tgagtcttcc tttgtgcgag cgggaactgc
     1741 tatcttcctg gcgcatacta tcccaaaatc cagagggacc agacgcgcgg agtggattga
     1801 caaattgact ctctatctgc tcgaccagga ttcgtatgtg cgaagcgttg cactgcggca
     1861 aatcgattcg atgattcaat cgggaaacaa atttgagccg agcctagatg caatcgcgaa
     1921 gctggctcaa gccgccaaaa aacaagaaga tctcattccg ttcctgcttc atttcgcaag
     1981 tgaaagcaag agtctgaaat ctgtgatcga aaaatcactc gcgtctccgc aatcagagaa
     2041 cgcggccttg attctaaaag cactgagaga accaactgtg aggcgctgct cgatttgcag
     2101 cagcctgaac cgagtcgagt cctggaatca cgaattgagg actcccgccc agatcaaaaa
     2161 aatgcgcgaa tcaggaaacc ttcgtttttg tactgaatgc gaaaacatct atcagttttc
     2221 atattcttca gaagtggaag acaaccatac gagcgaatca tttgaactgg aaagactgca
     2281 tcctgctgca gcactgaaaa tgctccaggg agaagacgcg atccaataca aagagcaact
     2341 tccgcggctc ctgcaaagcc tgcgctctga aacatctcat gtagatgaat ggattcgatc
     2401 agaggcggcc tggaaactcg cagaatatct tttatccacg gagcaatgga ccgaaatcga
     2461 tacactgtcg ctgcaaaacc agacctgctt gaaagagatc acactggccg ttgcgtccgc
     2521 agtgagatct ggcgtagacg caggccacac gctcaccact attaggcgag cgatgcaggc
     2581 agaacactgc gaaaccagga tgaactctgc cttcgttctt tcatgcatgt ctcttcaagg
     2641 ggaatcaaac gaacttccgg aaatcctgta ctccactgac tcttgcgttc tccagggatg
     2701 tctccaggca ttattcgcgc atgcatccag ggatctaccc tgtcctcatc tagatcgcat
     2761 cgccgcgctc actcttcacg atgacgatcg cgttcgcgcc ttcgcaaact acgtcgtgga
     2821 gagagcaaaa gaaaacggac agaagattcc ttttcttgtg cccaacttga tgaaagctct
     2881 cagaagcaag aacgccagaa tgaaacagga cggcatgcgt ttcctcagaa gcgaaatcac
     2941 acaacaaaat gcagcccaaa tacttggcga ggcagtctcc ctcttaaaag acagtgagcg
     3001 ctacggactc gtggaactga tcgaagcgtg cgcagaggca ggcgcaagcc tcgaaagata
     3061 cacaggccag tttatttcag atctacaaga ttccagaggg gatcttctgc ggttgttgac
     3121 tgtcgctgcg cggcagaatc cttcgatgat cggacgcaac gctaccgatg cgattgcaga
     3181 gtgtttgaac gactcacgac tcctccccgc cgcttcccat ctgttcgagt ttctttccaa
     3241 ttcaaaatgc gatcttacca ctgccatgcc agccatccaa tcgtttatag acagagcggt
     3301 cagtacggac tcatacacag agtcgttaat tcaggtcctg attcgctctc tcgccggatt
     3361 gtcagactat gagagcatca tgaaactact ttcgcacagg actacaacga tccgcctttc
     3421 cgttctatat agaatcaaag atctggcttc agagggactg caattccgcg caatcctcga
     3481 accactccag agcctgctaa aagatccgtc tgccggggtc cgcgaaatgg ctgaaaaggt
     3541 aatcactcag gtcagccagt catagaatct gcttgcacca cggcgggcag atcgcacaag
     3601 caatcatgaa gcgattcttc atcccgccag atttcaatct tgctgcagca tggtttgcgg
     3661 atgcaacact tcctgccagc ctgcgcacgt atcagaatat ccacgaactg gaaatcacac
     3721 cggcggacag agaaatgctg cgaagcctga gacacaatcg tttacttttc tttactaatc
     3781 atccaagcca ggcggagccc atgatcgcct atcacattgc caatgtgatg gggagtcgtt
     3841 ttcactacat ggccacaaga agagcgttcg atttcctgtt tggtgctgtt ggaaaacttt
     3901 tccagagcgt gggggcatac tccatccttc caggggttgc agatcgcgac tccatgcgaa
     3961 tgacgcgaca aattctctcc gcgcctggcg gcaagcttgc catctttcca gaaggggaac
     4021 ccatgtgcgg ggaaaatgac agcctcatgc cattccagcc cggattcttg aaactgggtt
     4081 tcgcggcctt gcaggacgcg cgcaaaacgg aaccgactgc gaacatttcc gtgctaccgg
     4141 gattcattaa gtatgtgatc aaatcatcgc gcgaacaaat catagaacat ctaacgcagt
     4201 ccattggcac gatcgaaaag aaactgggtc tagaagcggg gcatcgcaat ttgcttcgcc
     4261 agttcctgat ggttggacgc gtgctacttg aaaactcaga gaaagaatat ggaatcacgc
     4321 cccccgctga cgcggacttt gatttcagaa ttggaaaact gcgacacacg attctcgacc
     4381 gcgttgcgga aatgatgaaa ctgaaacatt acaatacagg tgcggatgca attcacaaac
     4441 tccgacatct tacaagtatc attgaacttt tagaagttgg ctatgaatct cctgagcttc
     4501 ctgttcttac tccgtctgaa ctggcagctg caaatcgcga atgcatcaaa gcatatgact
     4561 tcatcgtaat caagcgcgac tatctggttt caagacccac tccggaaaga ttttacgaat
     4621 ggttgcagcg cttcgaatcc ctggcgcttg gtcgcacacc gcgtgcactg ggcggcgaac
     4681 cacatccatt gcctcgcaga gcagtcatca catttgcaaa gccattcacg ctgggagaac
     4741 actacgacgc atttcaaaaa ggaaaaggcg aatacgtaga acagctaacc aatgaaattc
     4801 gcgcacaaat gcaggcgctt ttggatgcat ccgcgtcctt aacttacacg attgtagatc
     4861 cgggagacgt tggcggagtt tgattgcgaa accgttttaa cagagctaca cgatttcctt
     4921 tttcattgta acgcatagaa tcaaacacgc tctttgccat tgtgattcca cggccatgca
     4981 gatgcatttt cttattggct tccatggagt tgtctttgag gatttgagtg tgattgaatc
     5041 ctgctccctc gtcttgaacc acaatcaaca atctcctggg attgagagaa tacactactt
     5101 tgagctttct gctggaataa ggtgcttcaa actgtctgcg gcgcaacaga gccaaatagg
     5161 aaccatcttc tgtcgcattt gttttctctt cgtaggtaat gcctaaattt ccgtgctcaa
     5221 ttccattgat gatcatttca cttacgcaaa ttctaatctg caaagtcgtg cccgcgtcgc
     5281 aatgaagacc caggttattc gtaacgcgtt gactgatttg ttctgagaga gtcagatagt
     5341 taccgattac gaatttcata gactctcttt cgcaataagt ggcgagcata tcttctacca
     5401 tgctggcagc tttgccaaaa atcgcatatc ccccatcaag aggaacatac tccagagttg
     5461 tctcaagctc tctgggctca ttgtagcgag ttacgaactc cgtcttgaag ctcgcgacct
     5521 tctgattttg aatcactgat tcaatttgtt cagaaataaa ttcgcgcctg aaattcatgg
     5581 aacgatcggg gctcacgtaa atcaagtcaa ggatgctttt tccaatcaaa ctttctggct
     5641 gaaatccaag caaagaagac actgcctggt tgatagtagt aatgttgccc ttcgaatcaa
     5701 tagagaagat aatttcgcga gaactctcta caagatcccg atacttcttt tccgatttct
     5761 gaatctccgt gcgcgcggcc gtcaattcgt ccactcgcaa ttccagctca cgattcaaga
     5821 tgcgagacgc acgcaccatc ttctcaaggc gcatggttct ttctctaacg taacgcggag
     5881 aaatcacggc aaaaagtaga atgctcagca caatctgaaa tgcaagaaac gcaagaagga
     5941 tattgcgaat caaatcctcc atcaatgtgt taggcgttat gcgaacgtat agaggggcat
     6001 ccgttgatcg catgctggca acaagtggaa cactcaagaa tccgcttcca tcttctgaaa
     6061 gctccagggt tgcaggaaga atctcgcgtg ctgaaatgag aaatcgatcg aggctgaatg
     6121 caaacacgca tataccgggt atttgtggac ggccctgcgc gtctgccatc gccgcggcgg
     6181 tgaccaggta cggcttgtct ccgatgcgaa gaatcaaatt cgtttcccct cgctgcatgc
     6241 tgcgtgattc cacgaggaag cgaacaatgt ccggcggaat caccagagga gtatttccgg
     6301 ctcctgggaa ctcaaaccgg ggcgtatcag actgagaata aactgcgaaa taatcaaatg
     6361 agccctggag agtcggatcc ccttcaaaga gagctttcgc ttctgcccat ctgcccgtcc
     6421 tggcgaggtt ccatggttca gcccaccagg cgtaacctgt cacagcacca gagtggaaat
     6481 tccacctctg gtgaagatag ttgtcaaagg cgcgcgagaa ttcgcttttc ctttctgact
     6541 cgagtctgtg ggaaaaccag cgaccaaatc cgaagcctgc gatggcttct gctacaaaca
     6601 tgactgcaaa aaaaacgaga gcgtagacga tgagctggcg gccgcgctgc aactcttcga
     6661 gctgtgccat gtcccccggc tc
//
LOCUS       DAORFF010000159         6675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5913_length_6675_cov_3.280060, whole genome shotgun sequence.
ACCESSION   DAORFF010000159 DAORFF010000000
VERSION     DAORFF010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5913_length_6675_cov_3.280060"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..372)
                     /locus_tag="PKK76_08030"
     CDS             complement(106..372)
                     /locus_tag="PKK76_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68459.1"
                     /translation="MDREEDSAIKTALVKTGHTLLQLGIVRLAVRFFPGLTGFLTPAV
                     SVAYERVRKNTDLPGVVDQATAKLKNIQNERSVETTAEHKDSTH"
     gene            complement(447..767)
                     /locus_tag="PKK76_08035"
     CDS             complement(447..767)
                     /locus_tag="PKK76_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68460.1"
                     /translation="MTEKEKKEDKGTNLLQTMGESLSNIADTLTGRHLENRIREYTEL
                     YADVLLGLHRDLRNLEGRIEALPDKKEMDSMLKGLQIMRRLLIVSLGFSIISLGGVIW
                     TILR"
     gene            complement(764..1579)
                     /locus_tag="PKK76_08040"
     CDS             complement(764..1579)
                     /locus_tag="PKK76_08040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNL68461.1"
                     /translation="MRDKVVIITGSGSGIGRHLTGAFLNSGALVAATDINEDALKKAA
                     KKDGWDKERLISEKLDVRQEKSWKKLFSQVLKKWKRVDILINNAGYLMPAYIADADED
                     HFARHLDINARGTMIGTAHAARVMMEQKSGHIINIASLAGVAGIPGISAYSASKHAVR
                     GFSLAAAQEMRPHGVFVTTICPDAVKTPMLDLQKDYKEAAMTFSGGRYLTPVDIEGAI
                     RRAIDTRQREILLPGYRGMLAKIASAFPSLTATLGGVMQKQGQKKQAAFRKGS"
     gene            complement(1590..2642)
                     /locus_tag="PKK76_08045"
     CDS             complement(1590..2642)
                     /locus_tag="PKK76_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68462.1"
                     /translation="MDSFTPIKDFAGARSLPHPGDRLSEIKKRSRKFRDKLLAGKKAE
                     FYKTVDLVRVPYPTKYGLLNAFALPTPMMHILNRLYIVQFKQKGRLRTLLFSPSDIEN
                     NGETPFFKRLSRSFGPFESIGKRFIAPVLSTVEHALQSIGLSPEKIDYISYDHLHTQD
                     VRKWLGTRNMAPYFPNAKLLVMKQEWDSTKSLIGPQLDWYCPGGIEGIDPRKVILLKG
                     DTMLGDGVALIATPGHTEGNHSLVVHTDEGVLVSSENGVSADSYAPMSSRIPGVRRYA
                     AKAEMEVILNGNTLEGGLDQYISMVQEKEIAGPSARNPDFYNVVPSSELTHYWGFPGI
                     RPTFTFGPLEFGKIRT"
     gene            2742..3248
                     /locus_tag="PKK76_08050"
     CDS             2742..3248
                     /locus_tag="PKK76_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNL68463.1"
                     /translation="MAQKKKTPSKPKTAAKAARPRPTKPVFESRISFTTIGVRDFKRS
                     LDFYRDTIGFKLHSLQGDIAMFDVGGSVLALYPYHLLAEDAGLKPGGSGFSGIAMAYN
                     VRNAKDVDRFLGLLESRGTRILQKAEDKFWGGRSGYFADPDGYPWEIAWNPFIKMDAK
                     GRLILKKK"
     gene            complement(3279..4334)
                     /locus_tag="PKK76_08055"
     CDS             complement(3279..4334)
                     /locus_tag="PKK76_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNL68464.1"
                     /translation="MYFLIKMQYRELSNTGLLVSELCLGAMTFGGKDGIWGMIGNLDQ
                     KEVDELVGASIDSGINFFDTANVYAFGESEKLLGKALSSRRRDVIIATKVRGRMGPGA
                     NQTGLSRVHIMQSVEDSLRRLGTDYIDLFQIHGLDVLADFEDTLRTLDDLVRSGKVRY
                     IGASNLAAWHLMKCLGISKYQGLEPFKSIQSYYSLAGRDLEREIIPLLKSENVGLLVW
                     SPLAGGLLSGKFTKSNQDQEGRRAKFDFPPANKEKTFNIVDVLIKIGAAHNTSAARVA
                     LAWVLAQPAVTSVIIGAKRRDQLDDNIASTQLKLTEDELGQLDAVSALSPEYPGWMFQ
                     MQGSDRLPGQTRTIPKP"
     gene            4406..6076
                     /locus_tag="PKK76_08060"
     CDS             4406..6076
                     /locus_tag="PKK76_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68465.1"
                     /translation="MTQSKPRWAASGDWNAFFALFLDNIVNLVILSSILMLGFGFPKD
                     IIFHKILPGTALGVLFGDVVYSIMAIRLSRRTGQVVTAMPLGLDTPSTIGMAVAVLGP
                     AFLSLRGQGMSPDGAGDLAWKIGMASMLWMGGIKLIFSFIGATIQKVIPTAALLGSLA
                     GVGIVWLAADPLIHVYDLPFIGLIALAVLTFALIAKLELPFHLPGAAMAVVLGIVLYY
                     LLGELKLLPKDSMQVYKGFPAFEGIHFLLPFPTPAAFQEVFGRVIPYLPLAIPFGLLT
                     IVGGINVTEGARLVGDDFKTRDILLTEAFATIIAGVCGGVAQTTPYIGHSAYRQMGAR
                     SAYTLFTGIAVGLGASLGLVGFIVDLIPRAAVDPILIFVGLEILALSFRLTPKDHYMA
                     LGFALIPAILSFVHIKLKLLIQNVERVSGGISSTLSNLKPEQARPIQDALSQLIPVQI
                     SQEYQVIGVLGQGFILTSMVWAATVAYLIEKRFLAASIAMMAAAVFTFFGFMHSVFPD
                     GNVYLPWTIASQGISGRALAIPYELALCYIIMAALFPLLYAAQKKRAA"
     gene            6088..6672
                     /locus_tag="PKK76_08065"
     CDS             6088..6672
                     /locus_tag="PKK76_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68466.1"
                     /translation="MNSDYLTKLIDSYDDKQLRRMVFSMDIQGKPLFKIFEDLPDPIA
                     RSVVSLLDMIVEYTMSEEFARAHSFKKGLRCFEELYHLYHSTDFLKNFNQFAEKNGFY
                     SDEDEAVPMINEDGSLEKLYKYVNIKHITPGFGRDQEELDLKMRNNQDFIKSMIPGDF
                     HIKADACVPVFGPVTAGRYERGLEGFRVKFVFKA"
BASE COUNT         1485 a         1619 c         1721 g         1850 t
ORIGIN      
        1 gtggaagaat ggtcatccac aagatccagc cagcctggcc ctcgtgcgaa agcagagaat
       61 cgcgcgcgca aagagtttcc tctctttatg gcagtcctgt ttcagttagt gggttgagtc
      121 tttgtgttca gcggtagttt cgacgctgcg ttcattctga atatttttga gtttggcagt
      181 ggcttgatcc actactcccg gaaggtccgt gttttttctc actcgttcat aggccacgga
      241 aacggctggt gtcaaaaatc cagtcagacc cgggaagaat cggactgcga ggcgcacaat
      301 tccaagctgc agcagagtat ggcctgtctt gacaagtgcc gttttgatag cactgtcttc
      361 ttctctgtcc attacatact gtctggcaac gtcgcgaaat ttctcccccg ttccgcgtcg
      421 cgtttcgagc tcagtgagtt tttcttttag cgaaggatcg tccagatgac acctccgagg
      481 ctaatgatag aaaagccaag cgatacgatg agaagccttc tcatgatttg aaggcctttc
      541 aacatagaat ccatttcttt tttgtccgga agcgcttcga tcctgccctc aaggttgcga
      601 aggtctctat gaaggcccag gagaacgtcc gcatacaatt ccgtatattc gcgaattctg
      661 ttttcaagat gacgaccggt cagagtgtcc gcgatgttcg aaaggctttc gcccatcgtc
      721 tgtagcagat tggtgccctt gtcttctttc ttttcctttt ccgtcatgat cctttcctga
      781 aggctgcttg ctttttctgt ccctgctttt gcatcacgcc accaagggtt gctgttaaag
      841 aagggaacgc agaggcgatt ttggcaagca ttcctctgta tccaggcagt aagatttccc
      901 tttgtctggt gtcaattgca cgtctgatgg ctccttctat gtctaccggg gtcagataac
      961 ggccgccaga aaaggtcatc gccgcttctt tatagtcttt ctgtaaatcg agcatcggtg
     1021 tcttcacggc gtcggggcag atggttgtga cgaatacgcc gtgtgggcgc atttcctggg
     1081 ctgccgccag gctaaaaccg cgcaccgcat gtttggatgc ggagtatgcg gagattcccg
     1141 ggattccggc cactcctgca agcgaagcta tgttgatgat atgacctgac ttctgctcca
     1201 tcatcactct ggctgcatgc gctgtgccga tcatggttcc tctcgcgttg atatccaggt
     1261 ggcgcgcgaa atgatcttcg tctgcgtctg caatgtaggc cggcatcaag tagcctgcat
     1321 tgttgatgag tatgtccact ctcttccatt ttttcaaaac ttgcgagaag agcttcttcc
     1381 agctcttttc ctgtcgaacg tcgagttttt ctgagatcaa tctttctttg tcccagccgt
     1441 ccttctttgc tgcctttttg agagcgtctt cattgatgtc tgtggcggcc acgagagcgc
     1501 cagagttcaa aaacgctcct gtcaggtgtc ttccgattcc gcttcctgaa cccgtgatga
     1561 tgactacttt atcgcgcatg atgaactcct tacgttcgta tctttccaaa ttcgagaggg
     1621 ccaaatgtga atgttggtcg gatgcctgga aatccccagt agtgtgtgag ttcggaggaa
     1681 ggaaccacgt tgtagaagtc aggatttcgt gcggaaggtc ccgcaatttc cttttcctgc
     1741 accatggaga tgtactggtc gagtccacct tcgagagtgt ttccattcag gatcacttcc
     1801 atttctgcct ttgccgcata acgtctcaca ccagggatgc gagacgacat gggcgcgtaa
     1861 ctgtctgcgc ttacgccatt ttcgcttgag accagaactc cttcatctgt gtgaactacc
     1921 agggagtggt ttccttccgt atgtcctggt gtggcaatca aagcaactcc gtctccgagc
     1981 atagtgtctc ctttgaggag aatgactttg cgcggatcaa ttccttcaat cccaccgggg
     2041 caataccagt cgagttgcgg tccgatcagg ctttttgtgg agtcccattc ctgtttcatc
     2101 acaagtagtt tcgcattcgg gaagtaaggc gccatattgc gcgtgccaag ccatttgcgt
     2161 acgtcctgag tgtgcagatg atcgtaagaa atatagtcga tcttttcggg ggaaagtcca
     2221 atggattgta gcgcatgttc tacagtggaa agcaccggtg caataaaacg ttttccgatc
     2281 gattcgaacg gcccgaagga gcgcgaaagt cttttgaaga aaggagtctc tccgttgttt
     2341 tcaatgtctg atggagaaaa taggagcgtg cgaagtcgtc ccttttgttt gaactgcaca
     2401 atgtagagcc tgttaaggat gtgcatcatg ggagtgggaa gggcaaaggc gttgagaagg
     2461 ccgtattttg tgggataggg gacgcgcacc aggtctaccg ttttgtagaa ctctgctttc
     2521 tttccagcca ggagtttgtc cctgaactta cgtgatctct ttttgatttc agaaagtcta
     2581 tctcccggat gcggaagaga gcgcgcgcct gcaaagtctt tgatgggtgt aaatgaatcc
     2641 atagtgtgcc tgatgggaag ggggcgcgca tgctagagca ggtcaagcca aagtcgaatc
     2701 tgtagaagtt gacaacgagc gcacactata cctttgagac tatggcccag aagaaaaaaa
     2761 ctccatctaa accaaagact gctgcaaagg cagccaggcc cagacccacc aaaccggtgt
     2821 ttgaatctag aattagtttt acaacgattg gagtgcgcga cttcaaacga tcccttgact
     2881 tttaccgcga cacaatcggt tttaaacttc attcattgca gggcgatatc gcgatgtttg
     2941 acgtaggagg ttctgttctt gctctgtatc cgtatcattt gcttgcggag gatgcgggat
     3001 taaagccagg agggtccggg tttagcggca ttgcaatggc gtataacgtt cgtaatgcaa
     3061 aggatgtgga tcgatttctt ggccttctgg aatctcgcgg gaccaggatc ttacaaaagg
     3121 cagaagacaa attctggggc gggcgttcag gctacttcgc agatccggat ggttatccgt
     3181 gggaaatcgc ctggaatccg ttcatcaaaa tggacgcgaa gggcagactc atcttaaaga
     3241 agaaataacc ctcgcgcacc tacctcagaa acgttggctc agggttttgg aatcgttcgt
     3301 gtctgaccag gaaggcgatc tgaaccctgc atttgaaaca tccatcccgg atactcaggc
     3361 gaaagtgcgc tgaccgcgtc gagttgcccc agttcgtctt ctgtcagttt cagttgagtg
     3421 gaggcaatgt tgtcgtccaa ttgatcgcgt cttttggctc caatgatcac gcttgtaact
     3481 gccggctgtg cgagcaccca tgcaagtgct acgcgagcgg cggacgtatt gtgcgcggca
     3541 ccgattttga tgagtacgtc tactatgtta aacgtcttct ctttgttcgc tggagggaaa
     3601 tcgaatttgg ctcgtcttcc ttcctgatcc tgattggact tcgtgaattt tccagacagt
     3661 agcccgcctg cgagaggact ccacacaagc agaccgacgt tttcgctttt tagaagtgga
     3721 atgatttctc tttccaggtc ccgcccggct agagagtaat aggattgaat gcttttaaac
     3781 ggttcaagcc cctgatactt tgaaatgcca agacacttca tcagatgcca tgctgccagg
     3841 ttcgatgccc cgatgtaacg cactttgccg ctgcgcacaa gatcgtccag agttcgcaga
     3901 gtatcttcaa aatcagcgag cacatcgagt ccgtggatct ggaacagatc gatgtagtct
     3961 gtgccgagtc ttcgcaggct gtcttctaca gattgcatga tatgaacgcg cgaaagccct
     4021 gtttgattgg cacctggccc catgcgtcca cgcaccttgg ttgcgatgat cacatcgcgt
     4081 ctgcgactgg agagtgcttt ccccaggagt ttttctgatt ctccaaacgc gtacacgttt
     4141 gcagtgtcga agaaattgat tccagaatcg atcgatgctc ctacgagctc gtctacttcc
     4201 ttttgatcca ggttgccgat cattccccag attccatctt ttcctccgaa tgtcatagca
     4261 cccagacaga gttcagaaac gagaagaccg gtgttggata gttcgcggta ttgcattttg
     4321 ataagaaaat acatatggct cgcccgggcg agcacggctc tgaatttaca gttgcgcgcc
     4381 cggccagaat ttccagtgag tctccatgac gcaatcgaag ccgcgctggg ctgcatccgg
     4441 cgactggaat gcatttttcg cactgttcct ggataacatc gtgaatctgg tgatcctatc
     4501 tagcatcttg atgcttggat ttggttttcc caaagacatt atctttcata agattctacc
     4561 ggggacagct ctcggagtgc tcttcggaga tgtggtctac agcatcatgg ccattcgact
     4621 gtctcgtcgc acgggccagg tggtcacggc catgccattg ggactggata ctccatcgac
     4681 gatcggaatg gctgtcgcag ttcttggtcc cgcgtttttg agtctgcgcg gtcaggggat
     4741 gagtccggat ggtgcggggg acctggcgtg gaaaattgga atggcaagca tgctctggat
     4801 gggtggaatc aaactcattt tctctttcat tggcgccacg attcagaagg tcattccaac
     4861 agcggcgttg cttggttctc ttgcaggagt tggcattgtg tggcttgcgg cggatccgct
     4921 cattcatgta tatgatttgc cattcattgg actcatcgcg cttgcggtat tgactttcgc
     4981 actcatcgca aagctagagc ttccgtttca tctgccagga gcggcgatgg cagttgttct
     5041 gggtattgtt ctctactatc ttctgggaga attgaaactt cttccgaaag atagcatgca
     5101 agtctacaag ggattccctg cgtttgaagg aattcacttc cttcttccgt ttcctacacc
     5161 ggctgctttt caagaagtgt ttggtagagt gattccctat ttgcctctcg ccattccgtt
     5221 cggacttttg acgattgtag gtgggatcaa cgtgacagag ggcgcgcgtc tggttggaga
     5281 cgattttaag acgcgcgata ttctacttac ggaagctttc gcaacaatca ttgcgggcgt
     5341 ttgtggcggc gtcgcgcaaa ctactccata catcggtcat tcagcataca ggcagatggg
     5401 agcgcgcagc gcatacactc tcttcaccgg gattgcagtg ggcctgggcg cgagtctggg
     5461 gttggttgga ttcattgtgg atttgattcc gcgagcagcc gtggatccga ttttgatctt
     5521 cgttggtctt gaaattctgg ctctcagttt caggctcact ccgaaagatc attacatggc
     5581 acttggcttt gcgctcattc ctgccattct cagtttcgtg cacatcaagc tgaagcttct
     5641 gatccagaac gtagagagag tcagcggcgg aatttcttct actctgagca atctgaaacc
     5701 agaacaggcc cggccaattc aagacgctct ttcgcagctc atcccggtac agatatctca
     5761 ggaatatcag gtgattggag tgcttggaca gggattcatt cttacatcca tggtctgggc
     5821 tgccacggtt gcctatctca ttgagaaaag attcctggca gcaagcattg cgatgatggc
     5881 cgccgcggtt tttacttttt ttggattcat gcattccgtc tttccggatg gcaacgtgta
     5941 tcttccctgg acgatcgctt cgcagggaat ttcaggacgc gcgcttgcaa ttccatatga
     6001 acttgctctc tgttacatca tcatggccgc gctctttccg ctgttgtatg cagcgcaaaa
     6061 aaaacgggcg gcataaggag aagaaaaatg aacagcgact atttgacaaa actgatcgat
     6121 tcctacgacg acaagcaact cagacgcatg gttttttcca tggatatcca gggaaagcct
     6181 ctctttaaga ttttcgaaga tctacccgat ccaatcgcgc gaagcgtcgt ttctctcctg
     6241 gatatgattg ttgaatatac gatgagcgaa gagttcgcgc gcgcgcacag tttcaagaag
     6301 ggtctgcgtt gcttcgagga gctgtatcat ctttatcatt ccaccgattt cctgaagaac
     6361 ttcaatcagt ttgcggagaa gaatgggttc tactctgacg aagatgaagc agtgcccatg
     6421 atcaatgaag acggatcact tgagaaactt tacaaatatg taaatatcaa gcacatcact
     6481 cctggttttg gtcgcgatca ggaagagtta gatttgaaaa tgaggaacaa tcaggatttc
     6541 atcaagagca tgatccctgg agactttcac atcaaagcgg atgcctgcgt tccagtcttc
     6601 gggccagtca cagcaggtag atacgaacgt ggtctcgaag gatttcgcgt gaagttcgta
     6661 ttcaaggcct agtgc
//
LOCUS       DAORFF010000160         6661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5932_length_6661_cov_3.099455, whole genome shotgun sequence.
ACCESSION   DAORFF010000160 DAORFF010000000
VERSION     DAORFF010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5932_length_6661_cov_3.099455"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="PKK76_08070"
     CDS             complement(<1..1262)
                     /locus_tag="PKK76_08070"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNL68467.1"
                     /translation="MNDSQREELARRLLEQAPASWPEELRALRMDLRAMPEGLRSKLA
                     AMHGQQESRASFKTKVIRWPVAAAVSFSGAIAAALVVYFGVFRVDVPPMRIVFSDGDT
                     RKEGQPVKDGTLGEHERITVGEASSLVLGSADKKTEIGLRVRANSELTLPVLQADLVE
                     ARLVRGVVLSQLTNKESPARALLISTHNAQTRVTGTVFSVDVSRSSDTSLSTFEGTVS
                     IRRRWSDLEDLPENLLQRSEILSQSRDVFLKASASVPAGSQAVVSGMDFERRLGAIPH
                     LRKILSDPRISALRGRLKVSDSEVETAKKIIESNFPGPREREENLRLVETAFGQPPEV
                     TVVPAEDLSAMRQSLEQPSEAERKVRYEALLKQNPAPDRETFRRTATQALGKAPQEIL
                     LKNGETLFGSVFGLEGKYQVYTAQGIRVV"
     gene            complement(1259..1864)
                     /locus_tag="PKK76_08075"
     CDS             complement(1259..1864)
                     /locus_tag="PKK76_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNL68468.1"
                     /translation="MLLFSPRTILAIWGLPLPASASQSETLTEGDLESFLNQHGNHVY
                     TYLCVMCRDEDAASEALQNAYMKFIEQVRRSRVRRDTAAQYLLTIAKNDHFSRVRKES
                     REVELADDLQDGNAAQREARDHLARELQMVLLETAKDPQVPPDIAIIIRMRFLEGADL
                     ETICEKTGRSQATVYRLMEKALTILADACRRAGLHPEDAGL"
     gene            1905..2126
                     /locus_tag="PKK76_08080"
     CDS             1905..2126
                     /locus_tag="PKK76_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68469.1"
                     /translation="MLENAIPEIFSSGSATEGTTIEAGGVEFAWRRTGDQDHDISAQP
                     PGSFHIEAIVQARSDATARNEQNTDQTRT"
     gene            complement(2138..2794)
                     /locus_tag="PKK76_08085"
     CDS             complement(2138..2794)
                     /locus_tag="PKK76_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68470.1"
                     /translation="MKTSPIGEMHLIEFEDGTIEKIPNSRIRSLRRGATTWTRNQVKE
                     PEAPKVVTPEPSLPASRRSLAPILKSAVLPGWGQISEDRVKTGMAYGAASILVFQRYW
                     TYRQRHAAAQRDYNDPVPVGLVASQSVSGSLTLPQAAAINLAYLSQKEKQVYSLQRKG
                     NNMLALLPAIWAWNMLDIVYGGVPWEKRWLGSAPREQVPVLCISSGPGSITAAVRYYL
                     "
     gene            3224..5029
                     /gene="lepA"
                     /locus_tag="PKK76_08090"
     CDS             3224..5029
                     /gene="lepA"
                     /locus_tag="PKK76_08090"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNL68471.1"
                     /translation="MSVDQKLIRNFSIIAHIDHGKSTLADRILGLGGVTNARTSKDQI
                     LDSMDIERERGITIKMNSATFNYKTTDGITYIYNLIDTPGHVDFTYEVSRSLAACEGV
                     LLVVDASQGVEAQTLANLYLAMEANLRIIPVINKIDLPAADVPRCLDLIEKTLGLDPT
                     EAIAVSAKTGLNVPAILESIAKLIPAPSGDLNAPLRAMIYDSYYDSYCGAVMKVRIVD
                     GILRKGDRVSFMSHPRDYQVTEVGITAITNFPKDELAAGEVGYVIAGIKSVEDTRVGD
                     TVTLTSRPASVALPGYRDAKPMVFAGLFPVNGEDYPQLKDAIVKLKMNDAALTFEPET
                     SNALGFGYRVGYLGLLHMEIVQERLEREFNLALINSAPSVKYRITFTNGTVEEIDNPA
                     NWPDPQRIEKMEEPYVHANVLTPPEYVGNILALFQDKRGIQENMIYLADGKVQLIYAM
                     PLSELIFEFYDKLKSCSRGYASLDYEPHEYRESDLVKVDILVNAEPVDALAMIVHRGR
                     ADMRGRHLVDKLKELIPRQQFQIPLQAAVGAKVIARENISALRKNVTAKCYGGDITRK
                     KKLLEKQKEGKKRMKQIGSVEIPQEAFLALLKTDE"
     gene            5004..5915
                     /locus_tag="PKK76_08095"
     CDS             5004..5915
                     /locus_tag="PKK76_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68472.1"
                     /translation="MPCSKLTNRRSLLLVGLLCTLLHCRPETLLIYEIKTPEKVEEWT
                     ITPMRRGEAVHAISRSTPRGKSASDLEFRVFIGKKETLEEAAMRMGAKRENGAMQVQP
                     LRISVFKDRLPGVEDVRILDFSRFVIRRNNTVIEQIAFENFQKCCAHPALIDYRWFFG
                     AKYQGQLELELPDYMREMEEDTASPEEKEQAQKKKIAEWKPWAPSRIRSGDRLAAWLI
                     FPPLEIETDYILSHRDPGVMPDLPFRITMVRRNREILDTWTPEASERAISAEEREILQ
                     HEQEDTFIMHRQLQKHAELRDKEQKKN"
     gene            complement(5962..>6661)
                     /locus_tag="PKK76_08100"
     CDS             complement(5962..>6661)
                     /locus_tag="PKK76_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNL68473.1"
                     /translation="KPGMSRDDLINTNTKIVKSVAEGIKQYAPNAFVIVITNPLDAMV
                     HVMKKITGFPKNRVLGMAGVLDSARFRTFISMETGVSVEDITAFVLGGHGDTMVPLPR
                     YSTIAGIPLPEYPGMTAEKIDAMVKRTANGGGEIVNLLKTGSAFYAPASSAIAMAESI
                     LRDQKRVFPCAVLCEGEYGHNGIFMGVPVVVGSGGFERIIEIKLTAEEKAALDKSAAA
                     VKELMGVVDNSGLL"
BASE COUNT         1705 a         1823 c         1648 g         1485 t
ORIGIN      
        1 acgactcgaa tgccctgggc agtgtagacc tggtattttc cttccaggcc aaagaccgaa
       61 ccaaacaggg tctctccgtt cttgagaaga atttcctgcg gcgccttgcc cagagcctgg
      121 gtggccgtcc gcctgaatgt ctcacgatcc ggggcaggat tttgcttcaa caaggcttca
      181 taacgaactt tacgttctgc ttctgaaggt tgttccagag actgccgcat ggctgataga
      241 tcctctgctg gaactacagt gacttccggc ggctggccaa acgccgtttc cacaagacga
      301 aggttctctt ctcgctctct gggacctgga aaattgcttt caatgatctt cttcgcagtc
      361 tccacctcag aatcagaaac tttcaagcgg cctcgcaacg cggaaatgcg aggatctgaa
      421 agtatctttc gcaaatgagg gatcgccccg agtcttctct caaaatccat tccggaaacc
      481 actgcctgcg aacccgcggg cacggaagcg gaagctttca aaaacacatc ccggctctgg
      541 gaaaggattt cgcttcgctg cagcaaattc tccggtaaat cctccagatc ggaccagcgg
      601 cgacgaatgg aaacggtgcc ctcgaatgta gacagcgagg tgtcagaaga tcgactaacg
      661 tccactgaga atacagtacc ggtcacgcgg gtctgtgcat tgtgagtaga aatcagaagt
      721 gcccgagccg gactttcttt attggtgagc tggctcaaca caactccgcg aaccagtcgc
      781 gcttccacca gatctgcctg gagcacggga agagtcagtt cagaattcgc ccggactcga
      841 agaccgattt cggttttttt atccgcagat ccgagaacca gggaggaagc ctcccctacc
      901 gttattcgct catgttcacc aagcgttcca tctttgacag gctggccttc ttttctggta
      961 tcaccgtcag aaaacacaat cctcatagga gggacgtcca cgcgaaagac tccgaaataa
     1021 actacaagtg cagccgcaat cgcgccggaa aaagataccg ctgccgccac aggccatcga
     1081 atgacttttg ttttaaaact ggccctgctc tcttgctggc catgcatggc cgcgagcttc
     1141 gatcgcagac cttcgggcat ggcgcgcagg tccattcgca aggcgcggag ctcttcgggc
     1201 cacgatgcag gtgcttgctc gaggagtcgg cgagccaatt cttcgcgttg tgaatcgttc
     1261 atagcccggc atcctcagga tgaagtcccg ctcgcctgca tgcgtctgca agaattgtaa
     1321 gggccttctc catcaatcga tagaccgttg cctgagagcg acctgtcttt tcacagatcg
     1381 tttctagatc cgctccctca agaaaacgca tgcgaatgat gatggcaatg tccggaggca
     1441 cctgtggatc cttcgctgtt tccaggagaa ccatctgcag ttcgcgggcc agatgatctc
     1501 gcgcctcccg ctgtgctgca tttccatcct gcagatcatc cgcaagttcc acttcgcgcg
     1561 attctttgcg tactcttgaa aagtgatcat tctttgcgat tgtgagtaga tattgcgcag
     1621 ctgtgtcccg tcttactctt gatctacgca cctgctcaat gaatttcata taagcattct
     1681 gcaacgcctc tgacgctgca tcctcgtccc ggcacatcac acagagatac gtatacacat
     1741 gatttccatg ctgattcaag aaactctcaa ggtccccctc agtgagcgtt tcagactgcg
     1801 atgctgatgc gggaagaggc aggccccaga tggcaagtat ggtccgaggg gaaaacagga
     1861 gcatgattag aatacgatct cagcaaagaa gattcgcaaa gaaaatgctc gagaatgcga
     1921 ttccagaaat tttttcatct ggttcagcga ctgaaggaac aactatcgaa gctggcggcg
     1981 tcgaatttgc atggaggcgc accggtgatc aggatcatga tatatccgcc cagccccctg
     2041 ggtcctttca tatcgaagct attgttcagg cgcgaagcga tgcaactgcc cgtaatgagc
     2101 agaatacaga tcaaaccaga acgtagcacg tttgttttca taaatagtac cgcaccgcag
     2161 ccgtaatcga cccgggcccc gagctaatgc acaacactgg aacctgctcg cgtggagcgg
     2221 aacccaacca gcgcttctcc cagggaactc ccccgtaaac aatgtcaagc atgttccagg
     2281 cccatatcgc aggcaagagc gcaagcatgt tgttcccttt tctctgcaga ctgtacactt
     2341 gcttctcttt ctgggataga tatgctagat tgatcgccgc cgcttgcggg agagtcaaag
     2401 atcctgagac gctctgcgag gcgactaaac ccacggggac cgggtcgtta taatctctct
     2461 gggccgccgc atgcctctgt ctataagtcc agtacctctg aaacaccagg atagacgctg
     2521 ctccataagc catccctgtt ttcacgcgat cttcagaaat ctgaccccag ccaggaagca
     2581 ccgccgattt gagaatcggg gccagagagc ggcgcgacgc gggaagagaa ggctctggag
     2641 taacaacctt cggagcttcc ggctctttga cctgatttct agtccatgtc gtggcccctc
     2701 tgcgaagaga acggatgcgc gaatttggaa ttttctctat ggtgccgtct tcgaactcaa
     2761 tgaggtgcat ttcgccgatt ggcgaagtct tcacattgcg gagctggacg cctgaacgga
     2821 gtacaaccgt atccgcgagt gatcgcgcac tggcagcgca cacaagacag acgcatagaa
     2881 gccatcccgt cctcacatgg caacgttggc cttccgcgcg gtgaggcaaa gtgcttttcg
     2941 cacccatcca ggaaagcgat ctatcctctc cgaatagtaa ataaatctaa ctatagttca
     3001 ctggtagtca tgacatcttc cgatacattc attccgggcc ccgcccgtcg ctgttcctct
     3061 gcgcccacgg tgccgcctcg aatcgtcctg aaatatggcc tgggcctcga cttgcccggc
     3121 ttgcgcccga atccaggaaa tcctggccca tcggagaccg gcgcgtgtgc ccgaaaaaag
     3181 ggttcttcac cccggcgctt cagaaaaaat gccgtaaccc actatgagcg tcgaccagaa
     3241 actcattcgc aacttttcca tcattgccca catcgaccat ggcaagtcca ctctggcaga
     3301 taggatcctg ggtctcgggg gcgttacaaa cgcacgcacg agcaaggatc aaattctcga
     3361 ttccatggac atagagagag agcgcggcat cacaatcaaa atgaattccg caaccttcaa
     3421 ctacaagacc acggacggaa tcacttacat ctacaatctg atcgacacac ctggacacgt
     3481 ggacttcaca tacgaagtat ctcgttctct ggctgcctgc gaaggggttc tcctggtggt
     3541 agacgcaagc cagggagtgg aggcccaaac acttgccaat ctctatctgg ccatggaagc
     3601 gaaccttcgc atcattccgg tcatcaacaa gatcgatttg ccagcggcag atgtgccgcg
     3661 gtgcctggat ctgattgaaa agacactcgg cctcgatcca acggaagcca tcgcagtctc
     3721 tgcgaagact ggtctgaacg ttccagcaat tctagaatcc atcgcgaagc tcattcccgc
     3781 accgtcaggc gatctgaatg caccccttcg cgccatgatc tacgactcct actatgacag
     3841 ctattgcggc gcggtgatga aagtcagaat cgtagacggc atactgcgca aaggcgatcg
     3901 cgtcagcttc atgagtcatc ctcgcgacta ccaggtcaca gaggttggaa tcacagccat
     3961 caccaatttc ccgaaagatg aactggcggc tggcgaagta ggttatgtaa tcgcgggaat
     4021 caaatctgtg gaagacactc gagtgggaga tacagtcacc ctcacctctc gccctgcgtc
     4081 tgtggcactg ccagggtacc gcgacgcaaa accaatggtt tttgccggac tgtttcctgt
     4141 gaacggagaa gattatccgc aactgaaaga tgccatcgtc aaactgaaaa tgaatgacgc
     4201 ggcactcacg ttcgaaccgg agacatcaaa cgccctgggc ttcggttatc gcgttggata
     4261 tctgggcctt ctccatatgg agatcgttca agaacgcctc gaaagagaat tcaatctggc
     4321 gctaatcaac tctgctcctt ccgttaaata tcgcataacg tttacaaatg gtacagttga
     4381 ggaaatagac aatccagcaa actggcccga tccgcagcga attgaaaaaa tggaagaacc
     4441 ttacgtgcac gcaaatgtgc tcactcctcc agaatacgtt ggaaatattc tagcactgtt
     4501 tcaggacaag cgcggcattc aggaaaacat gatctatctg gcagacggca aagtacaact
     4561 catctacgcc atgcctcttt ctgaattgat ctttgaattc tacgataagc taaaatcttg
     4621 ttcgcgcggc tatgcttcat tggactacga accgcacgaa tacagggaat cagacctggt
     4681 caaagtggac atactggtaa acgcagagcc agtggatgct cttgccatga ttgttcatcg
     4741 agggcgagcg gatatgcgcg gacgccatct tgtggacaag ctcaaagaac tcattcctcg
     4801 ccagcagttt caaattcctc ttcaggcagc agtgggcgca aaagtgattg cgcgcgaaaa
     4861 catatctgcg ctccgtaaga acgtgactgc caaatgctat ggcggtgaca tcacgcgcaa
     4921 aaagaagctt ctggaaaagc agaaggaagg caaaaagcgc atgaaacaga ttggttctgt
     4981 ggagattccg caggaggctt ttcttgccct gctcaaaact gacgaataga cgttccttgc
     5041 tcctcgttgg tctgctttgc actctcttgc attgcaggcc agagactctt ctcatctacg
     5101 aaatcaaaac gccggaaaaa gtagaagagt ggaccatcac tccaatgcgc agaggggaag
     5161 cggtccatgc gatttccaga tcaaccccgc gaggcaagag cgcctcagac cttgaattca
     5221 gagtattcat tggaaagaaa gaaactctcg aagaagcggc catgcgcatg ggtgcgaaga
     5281 gagaaaacgg agcgatgcaa gtgcagcctc ttcgcatcag cgtatttaaa gatagactgc
     5341 cgggggtaga agacgttcgc atcctcgatt tttccaggtt cgtcattcgc agaaacaata
     5401 cagtcatcga acaaatcgcc tttgaaaact ttcaaaagtg ctgcgcccac cctgctttga
     5461 tcgactacag atggttcttt ggcgccaaat accagggcca gctggaacta gaactcccag
     5521 actacatgcg cgaaatggaa gaggacaccg cctcccccga agaaaaagaa caggcccaaa
     5581 agaagaagat cgcagaatgg aagccctggg ccccctctcg cattcgcagt ggagatcgtc
     5641 ttgccgcatg gttgatcttt ccgcctctgg aaatagaaac ggactacatt ctctctcatc
     5701 gtgatcccgg agtgatgcct gaccttccat ttcgcataac catggttagg cgaaacaggg
     5761 aaattctgga cacgtggact ccggaagctt ccgaaagggc catttcagcg gaggaaagag
     5821 agatattgca gcatgagcag gaagatacct ttatcatgca cagacagcta cagaaacatg
     5881 cagagctaag agataaagaa cagaaaaaga attgagccgc ccgcatcgcg ggcgactatc
     5941 catggttaga cgggctcgag attacagcag accggaattg tccactacac ccatcagttc
     6001 tttgactgct gccgccgatt tgtccagggc cgccttctct tctgcagtca gtttgatctc
     6061 aatgattcgc tcaaatccac ctgagcccac cacaaccggc acgcccatga agattccatt
     6121 gtgaccgtat tctccttcgc ataacactgc acaggggaaa acacgtttct gatcacgcag
     6181 aatggattcc gccatggcga tggcagaaga tgcaggcgcg taaaacgccg accctgtttt
     6241 tagcaaattg acaatctctc caccgccatt cgcggtgcgt ttcaccatgg catcgatctt
     6301 ctctgcagtc atgcctggat actcaggaag tggaatgccc gcaattgtag aatagcgagg
     6361 aagaggaacc atggtatctc cgtgaccgcc aaggacaaaa gcagtgatgt cttccacaga
     6421 aacgccggtt tccatagaaa tgaaagtgcg aaaacgagcc gaatcaagca cccctgccat
     6481 tccaaggacc cggttctttg gaaaacctgt gatcttcttc atcacatgaa ccatggcatc
     6541 gagagggttc gtaatgacga tgacgaaggc gttaggcgca tattgtttga tgccttctgc
     6601 tacgcttttg acgattttgg tgtttgtgtt gatcagatca tcacggctca tcccgggctt
     6661 g
//
LOCUS       DAORFF010000161         6653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5938_length_6653_cov_3.481055, whole genome shotgun sequence.
ACCESSION   DAORFF010000161 DAORFF010000000
VERSION     DAORFF010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5938_length_6653_cov_3.481055"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PKK76_08105"
     CDS             <1..746
                     /locus_tag="PKK76_08105"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNL68474.1"
                     /translation="QDSLNIRNSSSSQSDKILMEKADRTISHTIVRSLQTQFPEDKIV
                     SEDEGQVGSDGDFEWWCDPVDGTRNFIHGIPFFCISMGLTWRGSPVGGVVHVPAMGQT
                     GLTYHAIVGQGAFKNQNPIHVSTVSQLERAVMASGLPYRRKEIIGDILGDIFAFVTTG
                     IGLRRTGSVILDLCWIAEGKFDALWDRDISPWDSCAASVILFEAGGKISGYLGEKFDI
                     QQTRIVASNGILHEEAVSVLQKARKTTMN"
     gene            complement(765..1982)
                     /locus_tag="PKK76_08110"
     CDS             complement(765..1982)
                     /locus_tag="PKK76_08110"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNL68475.1"
                     /translation="MHRAVQITTWQRLTRPSSNEVPLLDGLRALSLVAVLLFHYWASL
                     RHFARRVPDSVDVFLLNLNTGVVLFFVLSGFLISRGLYNEHRRTGEISFRSFYVKRTF
                     RILPAYYFFLLFALLFIVINRAKVVHSTGILSPTADWYFDFLFISNYLPGIQGHTWTL
                     SIEEQFYLLFPLLAAFLFKLRRDRLLLALVGIYLIPLAFRILSMVTLPEGEYAPHVDR
                     ASLTRFDDIIVGILLAVGGKELFPRKFTALIGLAGLTLTAIVLLLPAQVPDWFVVVRP
                     NFTNLGFGAIVYWAIHREDVKVPKLVTAVARLSYSMYLWHMIAGAFGAGSVMHLIYDG
                     TKIGAGPLLRGLLVSALATGLCGYLSYRFVEQPFLRLREKLETPPEDRKPDDPSTDDQ
                     DSQTPGELVVTPF"
     gene            complement(2289..3374)
                     /locus_tag="PKK76_08115"
     CDS             complement(2289..3374)
                     /locus_tag="PKK76_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL68476.1"
                     /translation="MRRQVDSLSAGPKKTPGWIAATVALLFFVSCSMFQQNESSDKTN
                     SAVAFLLAGYLLNTQSAVPTVQRNSTYNVVKTTHVYAQALSHSNWGSTTTSVVDLSLD
                     LYRPENAPGNRPAMILIHGGGFMTGAKDDANMVDMANYFASRGWVCISIDYRLAAARG
                     TLSTAWGNYVLANVVSTEREQSYAMYPAARDAKAAVRWLYANAGTYGINTNYVTALGG
                     SAGSFLANMLGITNASDFRDESLSIVDTTLSTTNLSSGSTVHTVIDHWGGIHMMTYLQ
                     AIDGQSRFDAKDPPISIVHGTADAEVPFSQAQALRDAYISTGAPYEFNALVGAGHGAW
                     SATINGRSLSENAFLFITQKQNLKVLQ"
     gene            complement(3511..4053)
                     /locus_tag="PKK76_08120"
     CDS             complement(3511..4053)
                     /locus_tag="PKK76_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNL68477.1"
                     /translation="MRTPPFPPDASTAEDFLSVWRENRSELVSFIQKLPEDQFAKAPA
                     SGGWSASEIAEHLYLTQWNLARSLPIVLGGKFGKDKAELKSDLRYETLSKLLIRPRGA
                     KNPIEVGPKGGMDKAATLESLQKSMDKLDKAVSSKTKDQLQSRGMDHPFFGAISMFDW
                     LWVMTNHEATHIQTLISKYS"
     gene            4059..4742
                     /locus_tag="PKK76_08125"
     CDS             4059..4742
                     /locus_tag="PKK76_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HNL68478.1"
                     /translation="MVFRIAIATTFFHLMIAAAVLPLRMHIRVNMAMTLDGKTSAPGG
                     GWKPGKADRIRMDHIRAECDALLIGVRTLILDNPNVMVKHDIEKSPLPVIVVRKTLPP
                     GNLRIFEGPRPPLIIADESQKGYLQTKELDGLEWEWSRGTDARSIVKILEKRGLRTLL
                     VEGGPTMNDLFFAENLVDRLHLTLVSRVLGGKADTVDRMPVHRAFKLVKQESADEELF
                     LEYERVSQS"
     gene            complement(4745..4981)
                     /locus_tag="PKK76_08130"
     CDS             complement(4745..4981)
                     /locus_tag="PKK76_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HNL68479.1"
                     /translation="MQIPLSVPDIGDAEKIELASWKVKAGDQVTEGQELCELVTDKAA
                     FPLEAPHSGTIVSLSRSTGDLVKVGEVLAVLDRA"
     gene            5085..6062
                     /gene="nadA"
                     /locus_tag="PKK76_08135"
     CDS             5085..6062
                     /gene="nadA"
                     /locus_tag="PKK76_08135"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNL68480.1"
                     /translation="MQATQDLREIRAKLEATMMPMELDHAMPIIEEIHKLKKQKNAVI
                     LGHNYMTPDVYHGVSDYIGDSLGLARQAATTNADIILFNGVHFMAETAKICNPDKLVL
                     IADLKAGCSLAESITGADVRKLREQFPGAPVVTYVNCSAEVKAETDICCTSANALKVV
                     ESLPDETVIFLPDAYLAKNVQLKTKKRIVSWEKGKCMVHEQYTGKDILDYRKQFDDLL
                     VIAHPECDTDVTSVADFSGSTSQMEQFIRQNKQKNVLLVTECSMSDNLRSMFPDRTFL
                     STCHTCPHMKKITLEKVRDSLLYNQFAVEVPEDIRVRAKRSVDRMLEIG"
     gene            6062..6544
                     /gene="ispF"
                     /locus_tag="PKK76_08140"
     CDS             6062..6544
                     /gene="ispF"
                     /locus_tag="PKK76_08140"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNL68481.1"
                     /translation="MDVRVGSGLDFHRLIENKTRPLMIGGIEITSDLALEGHSDADVL
                     LHALADAILGACGFSDIGTYFSDKDPANKNLNSSIILKRSLDMMRQKGYRLSNCDITI
                     IGEKPRMSPHREQIVDRLAELCEIDRDRIALKATTTEKMGALGRSEGLGCSATVLVVK
                     "
BASE COUNT         1790 a         1600 c         1725 g         1538 t
ORIGIN      
        1 cgcaggactc attgaacatt cggaattctt cgtcctcgca gtcagacaaa attctgatgg
       61 aaaaagcaga tcgcacgatc agccacacca ttgttcgctc tctgcaaacg cagtttccgg
      121 aagacaaaat cgtatctgaa gacgaaggac aggtcggatc ggatggcgat ttcgaatggt
      181 ggtgcgaccc tgtagacggc actcgcaatt tcattcatgg tattccattc ttctgcatat
      241 ccatgggact gacgtggaga ggaagtcctg tgggcggagt cgttcatgtg cctgccatgg
      301 gacagaccgg gctcacgtat catgcgattg taggacaggg agcatttaaa aatcaaaatc
      361 caattcacgt ttctactgta agtcagctag aaagagcggt gatggcttcc ggcctgccct
      421 accgcagaaa ggaaatcatt ggagatatac tcggtgacat tttcgcattc gtgaccacag
      481 gaatcggcct tcgcagaaca ggctctgtga ttctggatct ttgctggatt gcggaaggta
      541 aattcgacgc gctctgggac agagacatca gtccctggga tagttgtgct gccagcgtca
      601 ttttatttga agcgggcgga aagatttccg gctatctggg agagaagttc gacatacaac
      661 agactcgcat tgttgcgtcg aatggaattc tgcacgaaga agcagtcagc gttttacaaa
      721 aagcgcgcaa gaccacaatg aactaaccgc gcgaaagtcg tgcatcagaa gggcgtgacc
      781 accagttcac caggtgtctg cgaatcctgg tcatctgtgc tcgggtcatc gggcttcctg
      841 tcttctggag gagtttccaa tttctcccga agacggagaa agggttgttc gacaaatcga
      901 taagacagat aaccgcaaag tcccgttgca agcgctgaaa cgagcaatcc tcgcaacaaa
      961 ggtcccgcgc caattttcgt tccgtcgtaa atgaggtgca tgacagaccc cgctccaaat
     1021 gcaccggcaa tcatgtgcca cagatacatg gaatagctga gtctggcaac tgccgttacg
     1081 agttttggaa ctttcacatc ttcacgatga atagcccagt agacgatcgc cccgaagcca
     1141 agattcgtaa agtttggcct gacaaccaca aaccagtccg ggacttgcgc gggcaacaga
     1201 agcacaatgg cggttaacgt caagccggcg aggccaatga gtgcggtgaa ctttctggga
     1261 aagagttctt ttccccctac tgccagcaga attcccacaa tgatatcatc aaaacgagtc
     1321 aggctggccc tgtccacatg aggagcatat tcgccttccg gtagagtaac catggagaga
     1381 atgcgaaacg caagtggaat cagatagatt ccaaccagtg ccaggagtaa gcgatctcta
     1441 cgaagcttaa acagaaaagc agccaggaga gggaagagaa ggtagaactg ctcttctata
     1501 gaaagagtcc acgtatgccc ctggatgccc ggtaaataat tggaaatgaa aaggaaatca
     1561 aaataccaat cagcggttgg tgataggatc cccgtgctgt gaaccacttt tgctctattg
     1621 atcacaatga acaacagcgc aaatagtaag aagaaatagt atgccggcaa aattcgaaac
     1681 gttctcttca cataaaaaga gcgaaaagaa atctccccgg tgcgccggtg ttcattgtag
     1741 agtcctctgg aaatcagaaa accgctgaga acaaaaaaga gaacaacgcc tgtgtttaga
     1801 ttgagcagaa acacatctac agaatctgga acgcgcctgg caaaatgacg aagcgaggcc
     1861 cagtagtgaa aaagtagaac tgctacgagt gacaaagcgc gaagtccgtc tagcaaagga
     1921 acttcgttgc tcgatggtct ggtcagccgt tgccaggttg tgatctgaac cgctcgatgc
     1981 atatgaaaca caacaactca ggtcatatct tttgcgcttt tatgttgagc cggatgcatt
     2041 tgtgtctaat tcgtcaccag gatgtaacga catgctagtc gcgcacgttt cacaaccgtt
     2101 cgcgccactg gaaaaacaaa acagcgcgtt cctcgaccag acctttgcag acaatgtgcg
     2161 cacacattgt gttgtttatg tccgatttcg tatctttcat gtaacgcttg gttagttgct
     2221 ccagcagatg gcatggtgta tggatggttc catggttatg gcgacctgag agagtgagcg
     2281 ggaagccatt actgaagcac tttcaaattt tgcttctgag taatgaagag aaaagcattt
     2341 tcagaaagag atctgccgtt gatcgtcgca gaccaggcac cgtgcccggc tccaaccagt
     2401 gcgttgaatt cgtacggcgc acctgtggaa atgtatgcgt ctctgagagc ctgggcctgg
     2461 gaaaagggta cctctgcgtc tgctgtacca tgaacaatgc taatgggcgg gtcttttgca
     2521 tcaaatctgg actgaccatc gattgcctgc agatacgtca tcatgtggat gccaccccag
     2581 tgatcgatca ctgtatgaac ggtagatcca gacgaaagat tggtagtgga aagagtagta
     2641 tctacgatac tcaagctttc atcgcgaaaa tcgctcgcat ttgtaattcc aagcatgttg
     2701 gcgagaaacg atcctgcgga tccaccgagg gctgttacat agtttgtatt gattccataa
     2761 gtcccggcat tcgcatacag ccagcgaacg gcagccttcg catctctggc agcaggatac
     2821 attgcatagc tttgttcccg ctccgtagaa acaacattgg cgagtacgta attcccccac
     2881 gccgtagaaa gagtcccgcg cgccgcagcc agacgatagt caatggaaat gcaaacccag
     2941 ccgcgacttg caaaatagtt ggccatatct accatgttcg catcgtcctt tgcgcctgtc
     3001 atgaatccgc cgccatgaat gaggatcatc gcaggcctat ttccaggtgc attctcaggg
     3061 cgatacagat ccagacttaa gtctaccaca gaagtggtgg tagatcccca gttggagtga
     3121 ctgagcgcct gagcgtatac gtgagttgtc ttcacaacgt tataggtgga gtttcgctgc
     3181 acagttggaa cggcgctttg tgtattgaga agatatccgg ccaggagaaa agccacagcc
     3241 gaattcgttt tgtcgcttga ttcgttttgc tgaaacatcg agcaagaaac gaagaacaaa
     3301 agcgcaaccg tagccgcgat ccatccgggt gttttttttg gccctgcaga aagagagtcc
     3361 acttgccttc tcatatctaa taaacgaaaa gacagaactt gtttgcaact attcttgcgc
     3421 gcgcacggag atgaaccctg gacaaatgga gaaaaagaac gaatgtccag ggtttttcag
     3481 aaggaatttt gcgaatctaa gtacaaatac ttaggaatat ttactaatta aagtctgaat
     3541 gtgagtggct tcgtgattgg tcatcaccca caaccagtca aacatgctaa tggcgccaaa
     3601 gaaaggatga tccatcccac gcgactgaag ttgatctttt gttttggaag aaacagcttt
     3661 gtccagcttg tccatggatt tttgaagcga ctcaagtgtg gctgctttgt ccattccacc
     3721 ctttggtcct acctcgattg gattttttgc accgcgcggc ctgatcagaa gtttgcttag
     3781 agtctcgtat ctgagatccg atttgagttc cgctttgtct tttccgaatt ttccgccgag
     3841 tacaatcggc aaggatctgg ccaggttcca ctgagtgaga taaaggtgct ctgcgatttc
     3901 agacgcgctc cagcctccgg aggcaggtgc ctttgcgaat tgatcctctg gaagtttctg
     3961 gatgaaactt acaagctctg atctattctc tcgccacaca gagaggaaat cttctgcagt
     4021 ggacgcatct ggtggaaaag gcggagttct catgactgat ggttttccgc atcgccattg
     4081 ccacaacttt ttttcatttg atgatcgcgg cggccgtgct tcctttgcgc atgcacattc
     4141 gggtcaacat ggccatgacg ttggacggga aaacgtcagc tccaggaggt ggatggaaac
     4201 cggggaaggc agatcgcatt cgcatggatc acattcgcgc ggaatgcgat gcacttttga
     4261 tcggggtgcg caccctgatc ctggacaatc caaatgtcat ggtcaagcat gatatagaga
     4321 aaagtccgct tccggtgatc gttgtgcgaa agacactgcc accgggaaat cttcgcattt
     4381 tcgagggacc gagaccaccc ctcatcatcg cagacgaaag ccagaagggc tacctgcaaa
     4441 caaaagaact cgatggtctg gaatgggaat ggtccagggg aacggatgcc cgttctatag
     4501 taaagattct ggagaaaaga gggcttcgca ctttgctcgt ggaaggcggt cccaccatga
     4561 acgatttgtt ctttgcagaa aatctggtag atcgtctcca cctaacactg gtttcgcgag
     4621 tactgggagg gaaagcagat acagtggaca gaatgccggt tcaccgggct tttaaactcg
     4681 tgaaacaaga aagcgcagac gaggagctct ttctggaata tgagagggtt tcgcagagct
     4741 agagtcaggc gcggtccagg acagcgagca cttcccctac tttcaccaga tcccctgtgc
     4801 tgcgcgatag agaaacgatg gtacctgaat gcggtgcctc aagtggaaag gcggctttgt
     4861 ctgtcacaag ctcgcagagc tcctgacctt ccgtgacctg gtcccctgct ttgactttcc
     4921 aggatgcaag ttcgattttt tcagcatctc caatgtcagg aacggaaaga ggaatctgca
     4981 tgggcgatgc tccgcccagg gcccatgatt ctcaaggaaa attccaaaaa agattgcggc
     5041 gggacaagtc gaaccatgtc taactactga gtagaggaaa tcatatgcag gcgacccagg
     5101 acctacgcga gatccgcgcg aaactagaag ctaccatgat gcccatggag cttgaccacg
     5161 ccatgcccat catcgaagaa attcacaaac tcaagaagca gaagaacgct gtgatcctgg
     5221 gacacaacta catgacgccg gatgtgtatc acggcgtgtc ggattacata ggggattcgc
     5281 tgggcctcgc caggcaggcg gccacgacga atgcagacat catactgttc aacggcgtac
     5341 acttcatggc agagacggcc aagatttgca atccggacaa actcgttctg atcgctgacc
     5401 tgaaggcggg atgctctctt gcagaaagca tcactggcgc agatgtgcga aagctcagag
     5461 agcaatttcc aggcgcacct gtagttacgt atgtaaactg ttctgcggaa gtaaaggcgg
     5521 agacagacat ttgctgcacc agtgccaatg ccctgaaagt tgtagaaagc cttccggatg
     5581 aaactgtgat ttttttgccg gacgcctatc ttgcaaagaa tgtgcagctc aaaacaaaga
     5641 aaagaatcgt ttcgtgggaa aaaggcaagt gcatggtgca tgagcaatac acgggaaaag
     5701 acatcctgga ctatcgcaaa cagttcgatg acctactcgt cattgcgcat cctgaatgcg
     5761 acacagatgt gacatcagtg gcggactttt ccggaagcac atcgcagatg gagcagttca
     5821 ttcgtcagaa caaacagaag aatgttctac tggtgacgga atgcagcatg agtgacaacc
     5881 ttcgttctat gtttccggat cgcacgtttc tttctacgtg ccacacctgc cctcacatga
     5941 agaagatcac tcttgaaaag gtgcgcgatt ccctgctcta caaccagttc gctgtggaag
     6001 ttccggaaga catccgggtg cgcgcaaagc gttctgtaga tagaatgctt gagattggct
     6061 gatggacgtt cgcgtgggat cgggtctgga tttccacaga ctcattgaga acaaaactcg
     6121 ccccttgatg attggcggca ttgaaatcac aagcgatctg gcactcgaag gccattccga
     6181 tgcagacgtg ctgctgcatg ctctggcaga tgcaattctc ggtgcgtgtg gattttcaga
     6241 catcggaacg tatttctcag acaaagaccc tgcaaacaaa aacctgaact cttctatcat
     6301 attgaagaga agtctggaca tgatgcgaca aaagggctac aggctctcta attgcgacat
     6361 cacgatcatt ggagaaaaac cgcgcatgtc tcctcaccgc gagcaaatcg tcgatcgcct
     6421 ggcagagctg tgcgaaatcg accgtgatcg aatcgcattg aaagctacta cgacagaaaa
     6481 gatgggcgcc cttggtagaa gcgaaggcct gggctgcagc gcgactgtgc ttgttgtaaa
     6541 atagaacgcg cggcccggaa caccgttagt cgctcttctg agctacaacc aggcgttggg
     6601 tcgagtaatg atgggcagac caggggggcc actcgttgca atcgggttcc ccg
//
LOCUS       DAORFF010000162         6645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5947_length_6645_cov_3.446737, whole genome shotgun sequence.
ACCESSION   DAORFF010000162 DAORFF010000000
VERSION     DAORFF010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5947_length_6645_cov_3.446737"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..4287)
                     /gene="rpoC"
                     /locus_tag="PKK76_08145"
     CDS             complement(76..4287)
                     /gene="rpoC"
                     /locus_tag="PKK76_08145"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNL68482.1"
                     /translation="MANVSVSDFESIRIQLASPDRIRDWSYGEVKKPETINYRTLRPE
                     RDGLFCERIFGTTKDWECYCGKFKSIRYKGVICDRCGVEVTHSKVRRERMGHVELATP
                     VAHIWYYRSVPSRMGLLLNLTINQLKSILYFEKYVIVDAGDSGRDKGELIDEEEYHEY
                     LDEYGDKFVAMIGGEAIKEMLTRIDVDAEIREIRAKIQEKGKVTDRRFLKRLEVLEAL
                     RESGNRPEWMMLEAIPVIPPELRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLAL
                     KAPEIIVRNERRMLQEAVDALFDNSRRKRSVKGKGNRPLKSLSDMLKGKTGRFRQNLL
                     GKRVDYSGRTVIVIGPDLKFHQMGLPKKMALELFKPFIMKRLVDLELAPNIKSAKKKV
                     ENEEKEVFEALEEVIKEHPVLLNRAPTLHRLGIQAFLPVLVEGKAIKLHPLVCHAFNA
                     DFDGDQMAIHVPLSPKAQLESWLLMLSPHNLLNPANGAPVCAPTQDMVLGIYILTSQL
                     AGDVGEGKVFADIDEIMYATSVNAVGFRAKVSVVHGGKMLQTTPGRVLFNATLPEGYP
                     YVNRPVSDKEVNRIIADVYDKHGVAAAVHMLDRVKDLGFHYATIFAPSISISDIRVSP
                     KKADLISGANKEVADVQKAHKNGVITNDERYKKIIEIWTRTNELITESMFAELEKDQR
                     GFNPIYIMAQSGARGSKQQIRQLAGMRGLMARPSGEIIELAIRSNFREGLGVLEFFIS
                     THGARKGLADTALKTADAGYLTRRLVDISQDVIITEVDCGTENGVRLRAVKEGDKTIV
                     SMGDRVFGRVVAEDVCDPVTSVVMIPRGTLITKAHSAKINSMGIESVFIRSALTCDTR
                     HGICTKCYGMDLARLKPVELGEAVGTIAAQSIGQPGTQLTMRTFHVGGVATNVQVKES
                     TYKLPHEAFILGIGGKLVTNPQKQQVFVNRGNINACRVSQVMDASKLINMRVEKGQAV
                     VPGEIIASGYAGEEKSVTAESQGTIDIRDGKFMILGEQMTIPVRAGAAIMVEAGQILD
                     KNETMATFDPFNEVVISEHSGTVQLQDIVEGKNLKKDEEGNFRIAEYKRERLSPRIVV
                     GGTDYTVPLNSLLLVKDGQKIEQGTILVKITSGVEKTRDITGGLPRVEELFEARRPKD
                     ASTLAEIDGRIEDFNEVVKEKKVLYIIPDSEEQEKIKVTIPVYKRVRVRHGDYVKGGD
                     QLDEGTLDPHDILRIKGINALHEFLIYEVQEVYRLQGVYINDKHIEIIVRQMLRKIEV
                     EDPGDTNFVAHQQVDRFTFREENERVVSEGGVPATARQILLGITRASLNTDSFISAAS
                     FQETTKVLTDAAIKGKKDALLGLKENVIIGHLIPAGTGLKHYRAVEIFKETYGDIERS
                     YKEKEEEAQRALLTQTRIDIQSSGG"
     gene            complement(4301..>6645)
                     /locus_tag="PKK76_08150"
     CDS             complement(4301..>6645)
                     /locus_tag="PKK76_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNL68483.1"
                     /translation="ATREFFGGSQLSQFMDQTNPLAELTHKRRLSALGPGGLRRERAG
                     FDVRDVHYSHYGRMCPIETPEGPNIGLIVSMSSYCRVNPYGFLETPYRKVASGKVSEK
                     IEYLTADIEERYNIAQANAPLEDNGHFKSKQISCRKRGDFPFCAPEEVQYMDMAPLQV
                     VSVSTSLIPFLEHDDANRALMGSNMQRQAVPLLVDEAPFVGTGIEEKAAYDSRNCVVA
                     KQDGMVLMVDSNKVVIRPTGGKKEDVTYFLKKFKRSNQGTCVNQRPVVSVYAAPADGK
                     VKITGDILEFTPAQGDPVQYFLKTDDTEFHAVVKSGASVSKGDLLAGQAVKGEVRDSQ
                     GILTQKGTVLADGHSIDNGRLALGKNVLVAFMPWEGYNFEDAILISERIVKDDVFTSI
                     HIDEYEIQARETKLGQEIITRDIPNLSDKAFRDLDENGVIRIGAEVKAGDILVGMVTP
                     KGESDLTPEYKLLHSIFGEKAREVRDTSLRMPNGSEGVVIDIKKYSREAGDELAAGVE
                     AMVKVFVAKKRKLQVGDKMAGRHGNKGVVARVMPEEDMPFLKDGTIVDIVLNPLGVPS
                     RMNIGQIFETMLGFAGKRLGTHFETPVFDGADEESIEKFLKQAELPTDCKFDIYDGRS
                     GLPFEGKVFCGYIYMLKLAHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRLGEMEV
                     WALEAYGASHTLQELLTVKSDDMLGRARIYEAIVKGVHSIRPGVPESFNVLMQELRGL
                     ALDVALYNQSDERVELSEIEDELGRGGRRNKIKIETIEKS"
BASE COUNT         1479 a         1725 c         1672 g         1769 t
ORIGIN      
        1 aatcggtttt gaatcagtag acaaaaaaag cggcgaaaac tcgccgcttt atgtcgcata
       61 gagactctcc ggcgattagc cgccggagga ctggatgtcg attctggttt gagtcagaag
      121 agcgcgctgt gcttcctctt ccttctcttt gtaagagcgt tcgatatcgc cataggtttc
      181 tttgaagatc tccacggcac ggtagtgctt cagcccagtt cccgcaggaa tcaaatggcc
      241 gatgatcacg ttttccttga ggccaaggag tgcatctttc ttgcccttga tcgcagcatc
      301 cgtaaggacc ttcgtggttt cctggaaaga agccgctgaa atgaaggagt ccgtgttcag
      361 agacgcgcga gtgatcccga gaaggatctg gcgcgcagtc gcaggcaccc caccttcgga
      421 aacgacgcgc tcgttctctt cgcggaacgt gaaacgatcc acttgctgat gagctacgaa
      481 gtttgtatcg cctggatctt ccacttcgat cttgcgaagc atctgacgca cgatgatctc
      541 gatgtgcttg tcgttgatgt acacgccctg gaggcggtaa acttcctgca cttcgtagat
      601 caggaattcg tgcaaagcgt tgattccctt gattcgcaga atgtcatgcg gatcaagagt
      661 gccttcgtcg agctgatcgc cgcctttgac gtagtcgccg tggcgaacgc gcacgcgctt
      721 gtatacaggg atggttactt tgatcttttc ctgctcttcg ctgtctggaa tgatatagag
      781 aactttcttc tcttttacca cttcgttgaa gtcctctata cggccgtcaa tttcagccag
      841 agtagaagcg tcttttggac gacgagcttc gaacaattct tccacacgag gaagaccacc
      901 ggtgatatcc ctggtctttt ctacgcctga agtgatcttc acaaggatgg ttccttgctc
      961 aatcttctgc ccgtctttta ctagaaggag cgaattcaga ggaacagtgt agtctgtgcc
     1021 gcctaccaca atgcgaggag aaagtctttc gcgcttgtat tcagcaatgc ggaagttccc
     1081 ttcttcgtct ttcttgaggt tcttgccttc cacaatgtcc tggagctgaa cggttccgct
     1141 gtgctctgag atcacaactt cgttgaacgg atcgaatgta gccatggtct cgttcttgtc
     1201 gaggatctgg cccgcttcta ccatgatggc cgcacctgct ctcacaggga tggtcatctg
     1261 ttcgccgagg atcatgaact tgccgtcgcg aatatcaatg gttccctgag attctgctgt
     1321 gacggatttt tcttcgcctg cgtatccgct tgcgatgatt tcaccaggca caaccgcctg
     1381 gcccttttca acacgcatgt tgatcagctt ggacgcgtcc attacctggc ttacgcggca
     1441 ggcgttgatg ttcccgcggt ttacaaagac ctgctgcttc tgcggattgg tgaccagttt
     1501 gccgccaatg cccagaatga atgcttcatg cggaagcttg tatgtgcttt ccttcacctg
     1561 aacgttggtt gcaacaccac ccacgtggaa cgtacgcatg gtcagctggg ttcctggctg
     1621 tccaatcgac tgcgccgcaa tcgttccgac cgcttcgccc agttcaacgg gcttgagtcg
     1681 agcgaggtcc attccataac attttgtaca aatgccatgt ctggtatcgc aggtaagtgc
     1741 ggagcgaatg aacacgcttt caatgcccat gctgttgatc ttagcactgt gcgcttttgt
     1801 gatcaaggtt ccgcgaggga tcatgactac ggaagtaaca ggatcgcata catcttctgc
     1861 aaccacgcgg ccgaatacac ggtctcccat ggatacgatg gttttgtccc cttctttcac
     1921 tgcgcgaaga cgaacaccat tttcagttcc gcagtccact tcagtgatga tcacatcctg
     1981 agaaatgtcc acgagacgac gagtcagata gcctgcatcc gcagttttca gagctgtatc
     2041 cgccagaccc ttgcgtgcac cgtgagtaga aatgaagaac tcaagtacac ccagaccttc
     2101 gcggaagtta gagcgaatgg caagttcaat gatttcgcct gacggacgag ccatgagacc
     2161 acgcataccg gccagctggc gaatctgctg cttggaacca cgcgcaccgg actgcgccat
     2221 gatgtagatt ggattgaaac cgcgctgatc tttttccagt tcagcgaaca tagattcggt
     2281 gatcagttcg tttgtgcgag tccagatctc gatgatcttc ttgtaacgtt cgtcgtttgt
     2341 aattacgccg ttcttgtgcg ctttctgaac gtccgccact tccttgttcg cgccagaaat
     2401 caggtctgcc ttctttggag agacgcgaat gtcagaaatc gaaatggacg gagcgaagat
     2461 agtcgcgtag tggaagccca ggtctttgac gcgatcaagc atgtgaacag cagccgctac
     2521 tccgtgcttg tcgtaaacgt ctgcgatgat ccggtttact tctttgtcgg aaaccggtct
     2581 gtttacgtac gggtagcctt ctggaagcgt tgcattgaag agcacacgac ctggagtcgt
     2641 ctgcagcatc ttgccgccgt gaaccacgga aaccttcgcg cgaaatccaa ctgcatttac
     2701 gcttgtggcg tacatgattt cgtcgatgtc agcgaatact tttccttctc caacatctcc
     2761 tgcgagctga gatgtgagga tgtaaatccc cagaaccata tcctgagttg gagcgcacac
     2821 tggagccccg tttgccgggt tcagcagatt gtgcggagaa agcatcaaga gccaggattc
     2881 aagctgcgct ttaggagaca gagggacgtg aatcgccatc tggtcaccgt caaagtccgc
     2941 gttgaatgca tggcaaacaa gaggatgaag tttgattgct tttccttcta caagaactgg
     3001 aaggaatgcc tgaatcccaa ggcgatgaag agtaggagcg cggttcaaga gaaccggatg
     3061 ttccttgatc acttcttcga gagcttcgaa aacttctttc tcttcgttct ctactttctt
     3121 cttcgcagac ttgatgttag gtgcaagttc caggtccaca agacgcttca tgatgaaggg
     3181 cttgaagagt tcgagtgcca tcttcttggg aaggcccatc tgatggaatt taagatctgg
     3241 tccaattacg attacagttc taccggagta gtctacgcgt ttgcccagaa ggttctgacg
     3301 gaaacggcct gtctttcctt tcagcatatc tgaaagtgat ttcaaaggac gattcccttt
     3361 tccttttacg gaacgcttgc gacggctgtt atcaaacaat gcgtccactg cttcttgcag
     3421 catgcgacgt tcgtttcgaa cgatgatctc gggagctttc agagccagaa gtcttttgag
     3481 acgattgttt ctattgataa cgcggcgata aagatcgttg aggtcagacg ttgcgaaacg
     3541 accgccttcg agctgaacca tgggacgaag ttctggcggg atgactggaa ttgcttcgag
     3601 catcatccac tcaggacggt tgcccgattc gcggagtgct tcgagtactt cgagtctctt
     3661 gaggaagcgg cggtctgtga ccttgccctt ctcctggatc ttcgcacgga tttcgcggat
     3721 ctctgcgtcc acatcaatgc gggtgagcat ttctttgatt gcttctccgc cgatcatcgc
     3781 tacgaacttg tcgccgtatt cgtccaggta ctcatgatac tcttcttcat cgatgagttc
     3841 gcctttgtcg cggcctgaat ccccggcatc cacgatgacg tacttttcga aatagagaat
     3901 gctcttgagc tggttgatgg tcaggttcag aagaagaccc atgcgagaag gcacagagcg
     3961 atagtaccag atgtgagcca caggagtggc gagttccaca tgacccatgc gttcgcgacg
     4021 cactttgctg tgagttactt ccacaccgca acgatcgcaa atcacaccct tgtagcggat
     4081 ggacttgaac ttaccgcagt agcattccca gtcttttgta gttccgaaaa ttcgctcgca
     4141 gaacagacca tcgcgctccg ggcggagtgt acggtagttg atagtctctg gcttttttac
     4201 ttcgccgtaa gaccagtccc ggatgcgatc cggagatgca agttggatgc gaattgattc
     4261 aaagtcgctg acgcttacat ttgccatttc ttattgcctc ttagctcttc tcgatggtct
     4321 cgatcttgat cttgttgcgg cggccgccgc gtccgagttc gtcttcgatt tcggacaatt
     4381 ctactctttc atcgctctgg ttatagagag cgacgtcaag agccaggcct ctgagttctt
     4441 gcataagaac gttaaacgat tctggaacac caggacgaat gctgtgaaca cctttcacaa
     4501 tcgcttcgta gatacgagcg cggccaagca tatcatctga cttcacagtc agaagttcct
     4561 gcaaagtatg cgaagcgccg tatgcttcga gtgcccacac ttccatttcc ccgagtctct
     4621 ggccgccgaa ctgagctttc ccgcccagtg gctgctgagt cacaagtgag tatggtccag
     4681 tagaacgagc atggatcttg tcgtctacga ggtgagcgag cttcaacatg tagatgtatc
     4741 cgcagaaaac ctttccttcg aaaggaagac cggagcggcc atcgtaaatg tcaaacttgc
     4801 agtctgtagg caattccgct tgcttgagga atttctcaat gctctcttcg tccgcgccgt
     4861 cgaataccgg cgtttcaaag tgagtaccaa gtctcttgcc tgcgaagcca agcatggttt
     4921 cgaaaatctg accgatgttc atacgagatg gaacaccaag cggattgaga acgatatcta
     4981 caatggttcc gtctttcagg aacggcatat cttcttccgg catcacgcgc gcaacaacac
     5041 ctttgtttcc atggcggccg gccattttat cgcccacctg aagcttgcgc ttctttgcaa
     5101 cgaatacttt gaccattgct tccacgcccg ctgcaagttc atcccctgct tcgcgagagt
     5161 acttcttgat gtcgatgacc acgccttcgg atccgtttgg catgcgaagg gatgtgtcgc
     5221 gcacttcgcg agctttttca ccgaagatgc tgtgcagtag tttgtattca ggagtcagat
     5281 ctgattcgcc tttaggcgta accataccaa cgagaatgtc tccggctttt acttctgcac
     5341 caatgcggat cacaccgttt tcgtcgagat cacggaatgc cttgtcgctg aggtttggaa
     5401 tgtcacgtgt aatgatttcc tgaccgagct ttgtttcgcg cgcctggatt tcatattcat
     5461 caatgtgaat agaggtgaat acgtcatctt taacaatgcg ttcgctgatg aggatcgcat
     5521 cttcgaagtt gtaaccttcc catggcatga atgcaacgag cacattctta ccaagtgcca
     5581 gccttccgtt gtcgatggaa tgaccgtccg caagaacagt tcccttttga gtgagaattc
     5641 cctgggaatc gcgaacttcc cctttgactg cctggccggc gaggagatct cccttggaaa
     5701 cagaagcgcc gcttttcact acagcgtgga attcagtgtc atctgttttc aggaaatact
     5761 gaactggatc tccctgagct ggagtgaatt caagaatgtc tcctgtgatt ttcaccttac
     5821 cgtctgcagg tgcagcatac acgctgacca ctggacgctg gttcacacag gttccctggt
     5881 tggatctttt gaatttcttg agaaaatacg ttacgtcttc ttttttcccg cctgttggac
     5941 gaatcacaac tttgttggaa tccaccatca gcaccatgcc gtcctgcttt gcgaccacac
     6001 agttgcgcga atcgtaggct gctttttctt cgatgcctgt tccaacaaaa ggagcttcat
     6061 ctacaagaag aggcacagcc tgacgctgca tgtttgagcc catgagtgcg cggttcgcat
     6121 catcgtgctc gaggaatgga atgagagaag tagaaacgct cactacctgc agaggagcca
     6181 tgtccatgta ctgcacttct tctggagcac agaatgggaa atcgccgcgc ttgcggcagc
     6241 tgatctgctt ggacttgaaa tgaccgttgt cttctagagg agcgtttgcc tgagcaatgt
     6301 tgtaacgctc ttcaatgtct gccgtgagat attcgatctt ttctgaaact ttgccggaag
     6361 caaccttgcg ataaggagtt tccaggaatc cgtatggatt cacacggcaa taagaactca
     6421 tcgaaacgat cagaccaatg tttggacctt ccggagtttc aatcggacac atacggccat
     6481 agtggctgta gtgaacatcg cgcacatcaa agccggcacg ttcacggcgc agaccaccgg
     6541 ggccaagggc gctcaagcgg cgcttgtggg tcaactcggc caacggattg gtttgatcca
     6601 tgaactggct caactggctg cccccaaaga attcgcgcgt ggctg
//
LOCUS       DAORFF010000163         6637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5965_length_6637_cov_3.929657, whole genome shotgun sequence.
ACCESSION   DAORFF010000163 DAORFF010000000
VERSION     DAORFF010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5965_length_6637_cov_3.929657"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PKK76_08155"
     CDS             <1..477
                     /locus_tag="PKK76_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNL68484.1"
                     /translation="RRTRTGVAPEERRKRMVLIAQDILDAGADPNARDNSGSTLLHLS
                     VLNSFPAFSQELLRHGARVDVVDDKGNLPLHLSFYQAIKSSDLSIVSELIERTPDINQ
                     ANSLGYTPLHFAAILDKPQVIRQLLSRGARRDIKNKDGKLALDLARSDECKELLRL"
     gene            complement(634..1191)
                     /locus_tag="PKK76_08160"
     CDS             complement(634..1191)
                     /locus_tag="PKK76_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68485.1"
                     /translation="MQRQLPLQSLFILIFLLGCGESKHLSEARKAALDSKSTDQEALQ
                     SIAAWCSWNPSETRYTGANVDPWQDGYRVTAGYILNPQRRFIKSVDDFKHAVRVEAAA
                     MVICMFRAGKSRKIRRIELSVALPLEQTSQLVNRFEIEEKDIVEIPNFANQRGPLDEK
                     GLEFYVADSKLKATTDNWEGIIVRN"
     gene            complement(1318..2133)
                     /locus_tag="PKK76_08165"
     CDS             complement(1318..2133)
                     /locus_tag="PKK76_08165"
                     /inference="COORDINATES: protein motif:HMM:NF020795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HNL68486.1"
                     /translation="MVSANDFVNKLVESVRKKKFADYIKASPEEVAESLRGKHIFVSG
                     EFATEKPGPGQSKVGGNPDLPESLPWLAEEETASPLAFIAQINLGEVHKHDLSGRLPE
                     KGMLWFFTIADGDRAYGEGIDESTSAVLYDPDPGILSPREMPAELLEENEFALIEERR
                     LLFGPSVSLSGYTDSGITDAIVDALREIGGRRGSVHILTQYRPSDYSSDEDEDSGDDS
                     EGSDAEMVSLIEMDGYEISSDIFVEGIIEFVLSEADLVAGKLERASCQFTAGT"
     gene            2264..5008
                     /locus_tag="PKK76_08170"
     CDS             2264..5008
                     /locus_tag="PKK76_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNL68487.1"
                     /translation="MIDTSSVGSFLNQLLDEDTPGAEILNDLRVDGFYTGNFGDPAFN
                     WGGFGFLPISGFGDGDIAALRPIFGRPVDQWPVMRVSHVGDPGKTFASSIKTFVAAHI
                     AMYGDVESDDLEDKRDALIEFAEFLGDTTGRARAVLDAMIADEFGGDDSDKYEIAEGE
                     GSMLASYYSLQENDEDSTAWQSFTKSFPEFAPGHISLFEILSDSEQPDLPALLPIAWR
                     VFNLDMQCDGSYKAARKAAKVLSENGGDKIYGQSHLWPAVIRMAAKKNCGKAWLLGAQ
                     ALEKDGNFQEAYNAYKSAAYWIGSEMEENLSINWVGARRCAEQLKDKNVVALFESLEV
                     PEGEDIEKVLTDYDREEEEEAEATAVQKSSEGFFESARTGNVEILKRELDNGAQIDLA
                     EPKSTLTALHFAAQEGHLDAVRLLFERGANINSKNFRSSTPLMLASRAGHLEIVRLLV
                     EKGADLEIVDGRDETALFLAAESGHVEVVKLLVAHGAKLNVRNISKQTPLFQAVFGDH
                     DEVATVLIEAGADVKLKDYSGESALHWAAGREAPELVKLLLKHGANPRAKADDGETPI
                     DRAEAENLDAIVAILKGETPPAETPFSDESMAALARMSKAWDMTLAKVKNVDELNWGY
                     SEQSDEDLATLHVLPKLRCIILMSCSAITDKGMKSLSRCTGLEEVIISNTPVTDVGIA
                     ELAGLPSLKKLWIDGTAVTDACTGHIAVMEKLEWLRLNELSQITDSAFAKLKSLKKLQ
                     RLDLSGSGISDEACEIIGGFNLESLDISKTQITDSGFEKIKNLKHLTSINFSDTKIGD
                     ACFEVLSGFQKLTEIDLDGTMVHGSGIEKLGKLKKLLSLKLGKCPFDVKNSASLKALK
                     TLRVLDLEGSKISDEAVPHLKTMKHLNALIIAKTKISPAGKKILKSALGAQTTFSWNE
                     "
     gene            4974..6125
                     /locus_tag="PKK76_08175"
     CDS             4974..6125
                     /locus_tag="PKK76_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNL68488.1"
                     /translation="MARKLLSAGTNEAMHLVGLAAFIAFALTFIPAVRARFIAKLFGG
                     IARQHRVHHWLGFTTGVIILIHLLYETLREPESAFSLEDPFLLLGWIASVLMFAGLFF
                     SVAKTLTHRFWIWFHWSLFISLVAAACHGFAYLHDATLDRLVFYTSSAIVATSTILIL
                     ASRQAFSAWTVTGINEITPNLHEILLKPEKKESRGFKAGSVVFAQFGAGFSRTWHPFS
                     VASCQFSPVMRLLIKSVGTDTSHLEDLREGSSMSIHGPFPEFNVPRKQDQVWIAAGVG
                     IAPFLGLTRCLDFQDSGKIRLIHYQAPDDWMLAGEFEGFQKRYPQFQWKNVMTARATF
                     DELNTLAEGLDNPAFLICGPPAFMKGARKFLKKQGVSSSNIHTEEFSPW"
     gene            6119..6433
                     /locus_tag="PKK76_08180"
     CDS             6119..6433
                     /locus_tag="PKK76_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HNL68489.1"
                     /translation="MVTLQNRLLVFVLALSPLVAQERIITSTELKANANGSSCWIQID
                     NYVYDITPYLSEHATTHEYALVKWCGKDATSGWKDKDGKNKSHSRKAATLLRKYRIGA
                     MR"
BASE COUNT         1756 a         1726 c         1608 g         1547 t
ORIGIN      
        1 cgccgaacgc gaactggcgt cgcccccgaa gaaaggcgca aaagaatggt cctgattgcg
       61 caggatatcc tggatgcagg agcggaccca aatgctcgcg ataactccgg atctaccctc
      121 ttacatctat cggttctgaa ttctttcccc gcattttctc aggaactact gcgccacgga
      181 gcccgggttg atgtcgtcga cgacaaggga aacctaccgc tgcatctttc tttttatcag
      241 gcgatcaaga gttcagattt gtctatcgtt tctgaactta ttgaaagaac accagatata
      301 aaccaggcaa actcactcgg ttacacaccc ttgcactttg cggcaatact cgataagccc
      361 caggtcattc gccaattgct ctcgcgagga gccaggcgag acatcaagaa taaggacggt
      421 aaactggccc ttgatctagc aaggagcgac gaatgcaaag aattgctgcg actgtgatac
      481 catcgcgtgt tggatgtcca gctcgcaaga ctctccaaga atcataatat ttttcaagta
      541 tccccccgcg cacgggggga tcgcgctgat agagagtcgc attttgaagg tgataatgcg
      601 accaggggga caagcccgtc cagagttctg agtctagttg cgcacgatga ttccttccca
      661 gttatcggtt gtcgccttta gcttactgtc cgcaacgtaa aactccaaac ccttctcatc
      721 gagggggccg cgctgatttg caaaattcgg aatttccaca atgtctttct cttcgatttc
      781 aaaacggttc acaagctgac tcgtttgttc cagcggaaga gccacggaca attcaatgcg
      841 ccttattttt ctgcttttcc ctgcccgaaa catgcagatc accatagctg cagcctcaac
      901 ccgcacggca tgcttgaaat catccacgct ctttataaag cggcgctgag gattcaatat
      961 ataaccggcc gttacccgat atccgtcctg ccatggatca acgttggccc cggtgtaacg
     1021 cgtttcgctt ggattccagg agcaccaggc ggcaatgctc tgcaatgcct cctgatccgt
     1081 cgattttgaa tccagagctg ccttccgggc ttccgagaga tgcttgcttt caccgcaccc
     1141 gagcagaaat atcagaataa agagtgattg caatggcaat tgacgctgca taaaaagacc
     1201 tcaacattag tatcaatccg cacaggagtt atcagattaa ctcccgcgcc aaacgctttt
     1261 ttcccgctca tagtagttga taaagtttac aaatagaaaa tccgcagcga aaggcgccta
     1321 agttccggca gtgaattggc aagatgcgcg ttcaagtttt cccgccacca ggtcagcttc
     1381 gcttaagacg aattcaatga ttccttctac aaaaatgtca cttgaaattt cgtacccatc
     1441 catctcgatc agggagacca tttccgcatc ggagccttca gaatcgtccc cgctatcttc
     1501 gtcctcatcc gaactgtagt cagatggccg atattgagtc agaatgtgaa ccgaaccgcg
     1561 tcgcccgccg atttccctca aagcgtcgac aatggcatcg gtgatgccgc tatcggtgta
     1621 acctgacagc gatacggaag gcccgaacaa aagtcgtcgt tcttcaatca aagcaaattc
     1681 attttcctca agtaactccg ctggcatctc gcgcggagaa agaatgccag gatccggatc
     1741 atacaaaaca gcagacgttg attcgtcgat tccctcaccg tatgctctgt caccatccgc
     1801 gatggtaaaa aaccacagca tccctttttc tggcaaccgc ccgctaagat cgtgcttgtg
     1861 cacttcgccc agattgattt gagcaatgaa ggccagaggg ctcgctgtct cctcttccgc
     1921 cagccacggt agcgattctg gcaggtccgg attgccccca acctttgact ggcccggccc
     1981 aggcttctct gtggcgaact caccagagac aaaaatatgt ttccccctca gactctccgc
     2041 cacttcttca ggcgacgcct ttatgtaatc ggcgaacttc ttctttctta cagactcaac
     2101 cagcttgttg acaaaatcat ttgcgctgac cataaaccct cacgactgac acaacgaatg
     2161 tgaatgatat ttattgcgta caaaatgccc cgacacacgg ttccttgatt tctgattgtg
     2221 gtccgaatgc ggcgggcaaa aatctgagaa acgagacgaa atcatgatag atacttccag
     2281 cgtaggaagc tttttaaatc aactactgga cgaagacaca cccggagcag aaatactgaa
     2341 tgatctccgg gtggacggtt tctacaccgg aaatttcgga gatcccgcat tcaactgggg
     2401 cggatttggc tttcttccca tttctggttt cggggatgga gacattgcag cgcttcgccc
     2461 gatcttcggt cgtcccgtag atcagtggcc cgtgatgcgt gtttcgcacg taggtgatcc
     2521 tggaaaaacc ttcgcctcct ctatcaagac gtttgttgca gcccacattg ccatgtacgg
     2581 cgacgttgaa tcagatgatc tcgaggacaa acgcgatgct ctcattgaat tcgcggaatt
     2641 tctgggggac accacaggaa gggcgcgagc cgttctcgat gcgatgatcg cagatgagtt
     2701 cggaggtgat gactcagaca aatacgaaat cgcagagggt gaaggaagca tgctcgcttc
     2761 gtactattcg ctccaggaaa atgacgaaga ctcaaccgcg tggcagagct tcacgaaaag
     2821 cttccccgaa ttcgcgcccg gtcatatctc tctttttgaa atcctgagtg attctgagca
     2881 accggactta ccggcccttc ttccgatcgc ctggcgagtt ttcaatctgg acatgcaatg
     2941 cgacggatcc tacaaagccg cccgaaaggc agcaaaggtt ttaagcgaaa acggtggaga
     3001 caagatctac ggacaatcgc atctgtggcc ggctgttatt cgaatggcgg caaagaagaa
     3061 ttgcgggaag gcatggcttc ttggtgcgca ggctctggaa aaagacggaa actttcaaga
     3121 agcatacaac gcctacaaga gcgcagcata ttggattggt tccgaaatgg aagagaacct
     3181 ttccatcaac tgggttggcg cgcggcgctg cgctgaacag ctcaaagata agaatgtagt
     3241 cgcactgttt gagtctctcg aggtgcccga gggagaagat atagagaaag ttctcactga
     3301 ttatgatcga gaggaagaag aagaggcgga ggctaccgca gttcaaaaat ccagcgaagg
     3361 gttttttgaa agtgcccgca caggtaacgt agaaatcctc aaacgcgaac tggacaatgg
     3421 tgcccagatt gatctcgcag aaccaaaaag tactcttaca gccctgcact tcgccgcaca
     3481 ggaaggtcac ctggatgctg tccgcctgtt attcgaaaga ggcgccaaca tcaattcgaa
     3541 aaacttcaga agctccacgc ctctgatgct cgcctcgcgc gcaggccatc ttgaaatagt
     3601 gcggctgctt gtagagaaag gggcagatct agaaatcgta gacggacgcg atgagaccgc
     3661 gctcttcctt gcggccgaat cgggtcatgt cgaagtcgtg aaactcctcg tggctcacgg
     3721 tgcaaaactc aacgtgcgaa acatctcaaa acaaacccct cttttccagg ctgtcttcgg
     3781 agaccatgat gaagtcgcca cggtcctcat cgaagcaggt gccgacgtga agttgaagga
     3841 ttattccgga gaaagcgcac tgcactgggc tgcaggtcgc gaagcacctg aactcgtaaa
     3901 gctactgttg aagcacggcg caaatccccg ggcaaaggcg gatgacggcg aaactcccat
     3961 cgatcgggca gaggcggaaa acctggatgc catagttgcc attctaaaag gagaaacacc
     4021 gcccgctgaa acgccattct cagacgaatc catggcagcg ctcgccagga tgagcaaagc
     4081 ctgggacatg acccttgcga aagtaaagaa tgtagatgaa ctgaactggg gttacagcga
     4141 acaatccgac gaagatctgg caacactcca tgttctgcca aagctacgat gcatcatcct
     4201 gatgtcgtgt agcgcaatca cagacaaggg aatgaaatct ctctccagat gcacgggcct
     4261 ggaagaggtg atcatttcaa atactccggt gacggacgtc ggaatcgcag agcttgcagg
     4321 gctgccatct ctgaaaaagc tctggataga cggtactgcc gtcacggatg cgtgcaccgg
     4381 tcacatcgcc gtgatggaaa aactcgaatg gctccgcttg aatgagttgt ctcagatcac
     4441 ggattcagca tttgcaaaac taaaaagttt aaaaaaatta caaagattgg atctttcagg
     4501 ctctggaatt tcggacgaag catgcgaaat cattggtgga tttaatctgg aaagtctcga
     4561 catttcaaaa acacaaatca cagacagcgg attcgagaag ataaagaact tgaaacactt
     4621 aacaagtatc aacttttcag atacaaaaat cggtgatgca tgtttcgagg tcttatccgg
     4681 ttttcaaaaa ctaactgaga tcgacctgga tggaaccatg gttcacggaa gcggcatcga
     4741 aaaactggga aaactgaaga aactactgtc cctgaaactt ggcaagtgcc catttgatgt
     4801 gaagaatagc gcttctctga aagcactgaa gactctacga gtccttgatc tggaaggatc
     4861 taagatttca gatgaagccg ttcctcattt gaaaacaatg aaacatctca acgcactgat
     4921 catcgcgaag actaaaattt cacctgcagg aaagaaaatc ctgaaatcgg cacttggcgc
     4981 gcaaactact ttcagctgga acgaatgaag caatgcacct tgtgggactc gcagcgttca
     5041 ttgccttcgc cttaactttt attcctgcag tgcgcgctcg gtttattgcg aaactattcg
     5101 gaggaattgc gcgccaacac cgggttcacc actggcttgg attcaccacc ggagtgatca
     5161 tcctcatcca ccttctatat gaaacactcc gtgaaccgga atccgcattt tctcttgaag
     5221 atccctttct tcttctgggt tggatcgcat ctgttttgat gttcgccggc ctcttcttct
     5281 ccgttgcgaa aactcttaca caccgattct ggatctggtt tcattggtcc ttatttatct
     5341 cccttgtcgc cgcggcgtgc cacggtttcg cttacctcca tgacgcaaca ctggacagac
     5401 tagtatttta cacaagttcg gcgattgtgg ccacgagcac gattctcatt ctagcatccc
     5461 gccaggcctt ttccgcgtgg accgtgaccg gcataaacga aatcacgccc aacctgcacg
     5521 aaattctgct caaaccagaa aagaaggaat cgagagggtt caaggcaggt tccgttgtat
     5581 ttgcgcagtt cggtgcgggc ttttccagaa catggcatcc tttctccgtc gcgtcatgtc
     5641 aattcagccc cgttatgcgg ctattgatca agagcgtggg tacggatacc tctcaccttg
     5701 aggatttgcg cgaaggaagt tccatgagca tccacggacc gtttcctgaa ttcaatgttc
     5761 cgcgtaaaca ggatcaagtc tggattgcgg cgggcgttgg aatcgcccct tttctcgggt
     5821 taacgcgttg tctggatttt caagattctg gcaagataag actcatccac tatcaggcac
     5881 cggacgattg gatgttggct ggagagttcg aaggctttca aaagcgatac cctcagttcc
     5941 aatggaagaa cgtgatgaca gcgagagcaa cttttgatga actaaacact cttgcagagg
     6001 ggcttgacaa tccagcattt cttatctgcg gtccacctgc ttttatgaaa ggagcaagaa
     6061 aattcttgaa gaaacaagga gtgtcatcgt ccaacataca tacggaggaa tttagcccat
     6121 ggtaaccctg cagaatcggc tcctggtttt cgtcctggcc ctgtctcctc tggtggcgca
     6181 ggaaagaatc attacgtcaa ccgaattgaa ggcaaatgca aacgggtcaa gctgctggat
     6241 tcagatcgat aactacgttt acgacatcac gccttatctt tcggaacacg caaccactca
     6301 cgaatacgca ctggtaaaat ggtgcggcaa agatgctaca agcggatgga aagacaaaga
     6361 cgggaagaac aaatcgcatt cccgcaaggc ggccaccctc cttcgcaagt accgcattgg
     6421 ggcaatgaga tgagcactgt gtcacaataa ggggtcgcta gacgccacct aacaacatga
     6481 agatggcttt tctgccacga cctgggaccg tgcttgcgat tctcaggaaa gatcgagaag
     6541 caatttgtcc agcaccagac agcactctgc cataccgtcg ggacccgcgc ctgattcgag
     6601 tgtggccttg cgtactcccg cagaagcata tgtgcac
//
LOCUS       DAORFF010000164         6619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_5992_length_6619_cov_3.427026, whole genome shotgun sequence.
ACCESSION   DAORFF010000164 DAORFF010000000
VERSION     DAORFF010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5992_length_6619_cov_3.427026"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1440
                     /locus_tag="PKK76_08185"
     CDS             76..1440
                     /locus_tag="PKK76_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HNL68490.1"
                     /translation="MNRKTATSALVIFFCAIGNLNAWSEHSIFMHSALSGMKEIAGAA
                     PVAAETLDSFLAKESKGLAQLLSEEESWARANIEGYPARPDDLAFDRSTETNLRKRFL
                     YALRVNPEAKMAMALQLMPGQDPAGRTFLTPRDVSVYKNPIWLAKIRFAQIRPGERVA
                     PLEILSTAADEPDLGLDIGLFEDSGTEWGSKMGFGKIPFGNANLEYSSQAPFHMTFYH
                     EAGIVYMLAGFLKHTYPEVRVRQFYALSRYAFRTGHPYWGYRFMGMGLHYVTDLTQPY
                     HARVLPGIGVLRMLWINLMDIAGRHQPKLDAIQLVSNRHSAIENFYQVILHRDSVAHD
                     MNHPYWKATVDESKDLSYGKFGRKYLKDVVTQESESRADALDSKLTDLIPSNLVSDPS
                     VEFAVANAGKDLTKVMEKEKPGSVDQLSLVLADFAASFGSHSRNFIRAVLALEDAYAS
                     SNAK"
     gene            complement(1442..2050)
                     /locus_tag="PKK76_08190"
     CDS             complement(1442..2050)
                     /locus_tag="PKK76_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68491.1"
                     /translation="MRKLIATFLTTTLSAAALVADPQLARTTAGKYVRLDDDGKWTYL
                     TESGLNEFLGKTQAGELKSTIFPGAGLKFQSGKWNLTNSTNKDAEFTLRDLGDGLYCM
                     FIPEKGSFSLELLKQAVVENAKSAVDSYNIVSEERRVINGKEFLILKAAITMKRTNFL
                     FKYALYSQDGYSLQTIVYGLEEPFKANEAIADKLLFEGVIVP"
     gene            2142..3884
                     /locus_tag="PKK76_08195"
     CDS             2142..3884
                     /locus_tag="PKK76_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNL68492.1"
                     /translation="MIQGNYFKDNEDLMLQFNSILDWKEIVAAYERGFEDAKTYEKTK
                     DERFANAPADVKSAVEYYESILEATGDLMGTYVAPRSAEMDRVGLKYENGKVTFPKAQ
                     EECYSTLRDAGLMPIGISRKNGGLGLPATVQSMICEIAARADAAFCLAFGNVNIVDIV
                     ERYGSDELIQEWIPDIAAGKYSAAMALTEPNYGSDLPSVQTKAEKGADGVWRLTGTKR
                     FITHACGYIDAPSIILTLARTGSPTSGARGLSFFLVQGKDVHVAGIEKKLGLHCSPTC
                     EVVFDNSPGQLIGEEGFGLVKYSMGMMNAARITIAAQSVGIAEAAYREAKKYASERIQ
                     FGKSIDKIPAVRKMLDRMEREIAGMRMLVLEATRSVDRYVWKKEHLIRHEGKSEKDAK
                     ADPEVKLWEKLADLFTPMAKYYASEGCVKIAYDALQIFGGAGYTEEYDVARIYRDARI
                     TTIYEGTTQLQVVAAIGGIVSGMAPAGNLREYIQQEMKSISPSAELKRSFDLFSEIAE
                     AYRDLPAGEEKSMLAFETVEAATRLLMGLLMERSLARTPDNAHRKEMCRGVSIDTIAT
                     LEGTRARMKLSAVK"
     gene            complement(3888..4667)
                     /locus_tag="PKK76_08200"
     CDS             complement(3888..4667)
                     /locus_tag="PKK76_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68493.1"
                     /translation="MQTLQYLSFDVAAGAVCAALFAARAARIELDPFVLACLFMAVLC
                     IYNVDHYLDARLRQQKGDARRTYHAEKKNWLLSLAILAFLAAAILMLRLPEIMIAGGL
                     GMLLFLCIYFIIINKFHLRFEKEILVSAGYTAGAWIAALPAFSISLLPYVAMMFCSCW
                     INVLVLSSHDLEVDRLRGEHNAIRAIGADVLGRLIAFIWICGMIAAALSLALDGRWIL
                     TAIAVAHMTLQGVLANQRFGQSARTIGDASYGLYALAWIVA"
     gene            complement(4667..>6619)
                     /locus_tag="PKK76_08205"
     CDS             complement(4667..>6619)
                     /locus_tag="PKK76_08205"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL68494.1"
                     /translation="QERRRHALSRKASRPKSDSACASSGGWQLRNLILLLILFGAACG
                     KEAERPDIEILREADSLEEARNSNGWEHAGRIVPNFGFKTSGVWARFPLENKNPEAWR
                     IIIEVGSAYLDRIDLFFISDGNVMRQSAGDTVDFRTRTVPHRNPASRLSLPPGSKSTV
                     YLHVTSKGTIMVPVRIWEADEFLYKAMPEYLIHGLYFGTIASLLVYNFMIFVFVRERS
                     YLYYCLYLSMILVSYLLLNGFAQQFFFQYSPFILNEGLLFVTALAIVSVALFNREFLQ
                     NKKVNSNMDKLMMSLIWFAVIVAISSLLLPFMISVRLITALMPLGSIALFLNAIMAAR
                     SGVQQSRYFILAWSALAAGSIVESATSFTLLPMSLAGRFGTQLGTVAEVLLFSLALGR
                     RIRRLSEEKGKAQARLSALEKDMELARAIQERILPHSIPVSEAEIKVTYIPLHAVGGD
                     FYDFHETGERSLGILIADVTGHGVSAALDSSTVKVAFRTVCSEIASPGIVLGKMNQFL
                     RTYIQYRFVSAFCIYLDFDAMQMKFAAAGHPPVLVLRQGKVIPLESEGMLLGVIQSFD
                     YKDATFDLQPGDKLILYTDGLYEDLPGEDYTIPLYAQVERCAATTDADAFGETLVHNL
                     SQMRTLLSDDITVITIDIPEPAVALR"
BASE COUNT         1711 a         1604 c         1726 g         1578 t
ORIGIN      
        1 cggctgggac aatccctcgg acaacagctg acattttagc ctggacctcg gacgaatatt
       61 tctataggat tgcttatgaa tcgaaaaacg gcgacttctg ctcttgtaat cttcttctgt
      121 gccattggaa atctgaacgc gtggagtgag cattctatct tcatgcattc agccctcagt
      181 ggaatgaaag aaattgccgg agccgcgcca gtggccgcgg agactctgga ttcatttctg
      241 gcaaaggaaa gcaagggact cgcgcagctt ctgtctgaag aagaatcctg ggcgcgtgcg
      301 aacatcgagg gatatcctgc cagaccagat gaccttgctt tcgatcgctc tacagagacg
      361 aacttgcgca agcgcttcct gtacgctctc agagtaaacc cagaagcgaa gatggccatg
      421 gcactgcagc tcatgccagg tcaggatccc gcgggcagaa cttttttgac tccgcgagat
      481 gtatctgttt acaaaaatcc aatctggctt gcgaagattc gtttcgcgca gattcgtcca
      541 ggagaacgcg tcgcacccct cgagattttg tccaccgccg cagacgagcc agacctgggt
      601 ctggacatcg ggctatttga agacagcggt actgaatggg ggagcaagat gggttttggt
      661 aagattccat ttggaaacgc caacctcgaa tacagttctc aagctccatt tcacatgacg
      721 ttctatcatg aggcgggaat cgtgtatatg ctcgcaggat tcttaaagca cacgtatcct
      781 gaagttcgag tgcgtcagtt ctacgcgcta tcgcggtatg catttcgcac gggtcatccg
      841 tactggggct atcgttttat gggaatgggt cttcactatg tgacggacct aactcaacct
      901 taccatgcgc gggtccttcc cggaatcggt gtgctcagaa tgctctggat caacttgatg
      961 gacattgctg gacgccatca accaaagtta gacgccatcc agctcgtctc caatcgccat
     1021 agtgcgattg aaaatttcta tcaagtgatt cttcacagag actcagtcgc gcacgacatg
     1081 aaccatccat actggaaagc aactgtagat gaaagcaaag acctgtcgta cgggaaattt
     1141 ggccggaaat atttgaaaga tgttgtcact caggaatctg aatctcgcgc agacgcgctg
     1201 gactcaaagc tcacggatct aattccatcg aacctcgtga gcgatccatc tgttgagttt
     1261 gcggttgcga atgctggaaa ggatcttacg aaagtgatgg agaaagaaaa gccagggtcc
     1321 gtggatcaat tgtccctggt tcttgctgat ttcgcggcgt cctttggatc gcattcgcga
     1381 aacttcatca gagcagtcct tgcacttgag gacgcctacg cgtcctcgaa tgcgaagtag
     1441 gtcagggaac gatcacgcct tcaaacagaa gtttgtctgc gattgcctcg tttgctttga
     1501 acggctcctc cagaccgtat acaatggtct ggagtgagta tccatcctga gaatagagcg
     1561 catacttgaa gagaaaattg gtgcgcttca tggtgatggc agcctttaga ataaggaatt
     1621 cttttccgtt gatcacacgc ctttcttctg aaacgatgtt gtaagagtcc actgcgctct
     1681 tcgcattttc aaccacagct tgcttgagca attccagact gaagcttccc ttttctggta
     1741 tgaacatgca atacaaaccg tcacccagat cgcgcaatgt gaactcagcg tctttgttcg
     1801 tgctgtttgt caggttccac ttgcccgatt gaaatttgag gccagctcca ggaaagatgg
     1861 tactcttcaa ttctcccgcc tgtgtctttc ccaggaattc gttcaggcct gattctgtta
     1921 agtaggtcca ttttccgtcg tcgtccaggc gaacgtattt gcccgcggtg gttcgtgcaa
     1981 gctggggatc agccacaagt gcagccgccg acagggtggt ggtcaaaaag gttgcgatca
     2041 gtttgcgcat ggctgatcag acagaacgcc gtcggtcagg tctagacctt tttttatatg
     2101 caacgcccca ttgagtccca gtttgacttt ggaggaaggc aatgatccag ggaaactatt
     2161 ttaaagataa cgaagacttg atgctccagt tcaattctat tctagactgg aaggaaatcg
     2221 tcgcggccta tgaacgcgga tttgaagacg caaaaactta tgaaaagaca aaggacgaaa
     2281 ggttcgcgaa tgctccggca gatgtgaaat ctgctgtaga gtattacgaa tctatcctgg
     2341 aagccaccgg tgatttaatg ggaacttacg tcgcaccgcg aagtgctgag atggatcggg
     2401 tgggcctgaa gtatgagaac ggaaaagtaa ctttcccaaa agcgcaggaa gagtgttatt
     2461 ccactctgcg tgatgctggc ctcatgccga ttggaatcag tcgtaagaac ggaggcctgg
     2521 gtctgccggc aactgttcaa tccatgatct gcgaaattgc tgcgagagcg gacgcagcct
     2581 tctgtcttgc gtttggaaac gtgaacattg tagatattgt agagagatat ggctcagacg
     2641 agttgattca agaatggatt ccggacattg cggcaggaaa gtacagcgct gcgatggcat
     2701 tgacggaacc aaactacgga tcggatctcc ccagtgttca aaccaaagca gagaagggcg
     2761 cagacggcgt ctggcgcctg actggaacca aacgattcat cactcatgca tgcggctata
     2821 ttgatgctcc aagcatcatt ctcactctgg ctcgcactgg atctccaacg agcggagctc
     2881 gtggactttc ctttttcctg gttcagggca aagacgtgca tgtggcggga attgaaaaaa
     2941 agctcgggct tcactgttcg ccaacgtgtg aagtggtgtt tgacaactct cctggccaac
     3001 tgatcggaga agagggcttt ggtcttgtga agtactctat gggcatgatg aatgctgcgc
     3061 gcattaccat tgcggcacaa tctgtgggca ttgcagaggc agcctaccgt gaagctaaga
     3121 agtacgcatc agaaagaatt caatttggaa aatccattga taagattcct gcagtgcgca
     3181 agatgctcga ccgcatggag cgcgaaatcg ctggaatgcg aatgctggtt ctcgaagcaa
     3241 ctcgatctgt ggatcgctat gtatggaaga aagagcatct gatcagacac gaagggaaga
     3301 gtgaaaaaga cgccaaagca gatccggaag tgaaactctg ggaaaagctc gcggatttgt
     3361 ttacacccat ggcaaaatat tacgcaagcg aaggctgtgt gaagattgca tacgacgcgc
     3421 tacagatttt cggaggcgcg ggctatacgg aagagtatga tgtggcacgc atttatcgcg
     3481 atgcacgcat tacaaccatt tacgaaggca caacgcaact ccaggtagtg gcagccatcg
     3541 gtggcattgt ttctggcatg gccccagcag gaaatctgcg cgaatacatt cagcaagaaa
     3601 tgaagtcgat ttctccgtca gcggaactca agagatcctt tgatttgttc tctgagattg
     3661 cagaggcata cagagatcta ccagccgggg aagagaaaag tatgcttgcg tttgaaacag
     3721 tggaagcagc cacacgtctc ctgatgggcc ttctcatgga gcgctctctg gcgcgtactc
     3781 ctgataacgc tcatcgcaaa gaaatgtgtc gcggtgtgag catcgatacg attgccactc
     3841 tggaaggaac acgcgcgcgc atgaagcttt ctgcagtgaa gtaaacgtca ggcgacgatc
     3901 caggcaaggg catagagccc gtaactcgca tccccaatgg ttcgggcgct ctgcccgaac
     3961 cgttgattcg caagcactcc ctgcaaagtc atgtgtgcca ctgcgattgc ggtgaggatc
     4021 catctaccgt ccagggcaag cgaaagtgct gccgcgatca ttccgcaaat ccagatgaat
     4081 gcaatcaagc ggccaagtac atccgctcca atggcacgaa ttgcattgtg ctctccgcga
     4141 agcctgtcca cttccaggtc atggctggat agtaccaata cattgatcca gcaggagcag
     4201 aacatcatcg ctacatacgg caagagcgat atagaaaagg cgggcagagc cgcgatccac
     4261 gcacctgcag tgtagcctgc agaaacaaga atttcttttt caaatctgag atggaatttg
     4321 tttatgatga tgaagtaaat gcacaggaac agcaacatac cgagtccacc tgcgatcatg
     4381 atctcaggca ggcgaagcat caaaatcgcg gccgcgagaa aggccagaat ggcgagggac
     4441 aagagccagt tctttttttc tgcatgatag gttctgcgtg cgtctccttt ctgctgtctg
     4501 agtcttgcgt ccagatagtg gtccacgtta tagatgcaca gtacggccat gaatagacac
     4561 gccagaacaa aaggatccag ctctattctg gcggcccgcg ctgcaaacag agccgcgcag
     4621 accgcacccg ccgcgacgtc gaagctcagg tactgcagtg tttgcatcag cgcagagcta
     4681 ccgctggttc tggaatatcg atcgtaatca ccgtgatatc atctgacaga agagttcgca
     4741 tctggcttag attgtgaacg agtgtttctc cgaacgcatc tgcatctgtg gtggcagcac
     4801 acctttccac ctgggcatag agcggaatcg tgtagtcttc acctggaaga tcctcgtaca
     4861 atccgtccgt atagagaatc agtttgtccc cgggctgaag gtcgaaggtg gcatccttgt
     4921 aatcgaacga ttgtattacg cccagcagca ttccttctga ttcgagaggg attactttac
     4981 cttgccgcag tacaaggaca gggggatgtc ctgcagcggc gaacttcatt tgcatcgcat
     5041 cgaaatcaag gtagatgcaa aatgcgctca caaatctgta ctggatgtat gtgcgaagga
     5101 attgattcat cttaccaagg acaattcccg gagatgctat ctctgaacaa actgtgcgaa
     5161 atgccacttt gactgtggat gagtcgagtg cagcagaaac accgtggccc gtcacgtctg
     5221 cgatcaaaat gccgagagat ctttcgcctg tttcatggaa gtcgtaaaaa tctccaccca
     5281 ccgcatgcag gggaatatat gttactttta tttctgcttc gcttacggga atcgaatggg
     5341 ggaggatacg ctcttgaatc gctcgcgcca gctccatatc tttttccagt gcggatagtc
     5401 tggcctgtgc ttttcctttt tcttctgaaa ggcgcctgat tctgcggccg agagcgagag
     5461 aaaaaagaag cacttctgcc actgtgccca gctgcgttcc aaatcggcct gcaagagaca
     5521 tgggcagcag agtgaaagag gtagcagact caactatgga tcctgccgcc agtgcgctcc
     5581 aggccaggat aaagtagcga gattgttgca ctccgcttct tgccgccatg attgcattta
     5641 gaaacaatgc gatcgaaccc aggggcatca gcgcagtgat gagacgaaca gagatcatga
     5701 agggaaggag caaggaggat atggctacaa tgactgcaaa ccagatcagg gacatcatga
     5761 gtttgtccat gtttgaattt actttcttgt tttgcaggaa ttcccggttg aacagcgcca
     5821 cactcacgat cgcaagagcg gtcacgaata gtaaaccttc gttgagaatg aaaggagaat
     5881 actggaagaa aaactgctgt gcgaagccat tgaggagcaa ataggaaacc agaatcatcg
     5941 acagatacag gcaatagtac agataacttc tttcacgcac gaaaacaaag atcatgaagt
     6001 tatagacaag taaagacgcg atcgttccaa aatacaggcc atgaatcaag tattcaggca
     6061 ttgccttgta caaaaactcg tccgcttccc agatccgaac cgggaccatg atggttcctt
     6121 tggacgtcac gtgcaggtag accgtgcttt tggatcccgg aggcaatgag agacgggacg
     6181 ctggattgcg atgtggcacg gtgcgcgtgc gaaaatcaac cgtatctccg gctgattggc
     6241 gcataacgtt cccgtccgag ataaaaaaca gatcaattcg atctagatac gcagacccca
     6301 cttcaatgat gattcgccat gcttctggat tcttgttttc gaggggaaag cgggcccaga
     6361 ctccagaagt cttgaagccg aaattgggca cgattctgcc tgcgtgctcc cacccattgc
     6421 tatttctagc ctcttcgagg ctgtctgctt ctcgcaggat ttcgatgtca ggtctttccg
     6481 cttctttgcc gcatgccgcg ccaaacagaa tcaaaagtag aatcaaattt ctcaattgcc
     6541 acccgcctga actggcgcat gctgaatctg atttcggccg gcttgctttg cgcgatagag
     6601 catgtcgtct gcgttcttg
//
LOCUS       DAORFF010000165         6599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6015_length_6599_cov_3.715770, whole genome shotgun sequence.
ACCESSION   DAORFF010000165 DAORFF010000000
VERSION     DAORFF010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6015_length_6599_cov_3.715770"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2149)
                     /locus_tag="PKK76_08210"
     CDS             complement(<1..2149)
                     /locus_tag="PKK76_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HNL68495.1"
                     /translation="METVEGTLLDVYNFNDEIHLWIKTDSRALHFIDSFHPIIFADGP
                     VDLLKKLVTRFHELGAIHGQPVWTIRNHFYDNSPRRVLKITIAKPAILNRVRRRLYSF
                     YGKLDLYHTDIEIPVGYMFHKNIHPLSRVRIDYTTSGQTRNIERIVSLDRITDLEFDI
                     PPFKQIGIKLEKSHRLGLKDNRLIFETSRGTIALSAHNQKECLLQINRILAEEDPDII
                     VSSYGDQIIFPELFTLAQTHNIPLLFDRHGHHSLRKIVREGTSFNTYGSWIFRAASYP
                     LFGRLHVDAHNSFVFKESRLVGIFELARLARTPIQRMSRQSTGGALTSIETHVAIRKH
                     YLVPWQKSAVEKPKTAYDLLAVDKGGLIFVPNASETSVFENVAQLDYSQMYPSIMVNH
                     NISPECVNCPCCANDPSAPKVPEAGYHICKRRRGVVSDALAHILFRRKYYKTRLRELK
                     AQPETQHTISQIDFYDARQDSLKWMLVTSFGYLGYRNAKFGRIESHESVTAFGREKLL
                     LAKEIAEEEGYTLSHAITDCVFLHRQGQVLDKESILRMAESITNASGIEMSLDGIYSW
                     VIFLPSRQDRLLPVVNRYCGRFLDGSIKFRGIAARRKDTASFIRNAQLELLEIMAKGT
                     SIEHLRSLHSEVMELYDRKCSELRNGTVPWEQLLLRKTTSREFKDYQVKNATYLAMEQ
                     LSDLSLKVQAGEKVRYVVLNTKHPDRRKRYLSEE"
     gene            complement(2136..2450)
                     /locus_tag="PKK76_08215"
     CDS             complement(2136..2450)
                     /locus_tag="PKK76_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017809851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68496.1"
                     /translation="MGRTHLPYSMQIDLVEDRLKKLRRGLRKEDQKIFDRLMRTARMQ
                     IQAGAMASSPNPFDTMALTMLIEIQRQIDVQSEQIKALQSVVLEIKNQSLLHTEKEDG
                     NS"
     gene            complement(2454..3104)
                     /locus_tag="PKK76_08220"
     CDS             complement(2454..3104)
                     /locus_tag="PKK76_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68497.1"
                     /translation="MLVRPTDSLRYFTAFSGKTGLLTGRSGIFHLSHLWQTHLSLLGQ
                     RVLVIDCAIRFNAFYLADEAMRMGLQTEALLQSILVQRAFTPYQILDVLYGILDAAHK
                     PPDSTYFLLAPFKQFFDGDVSDEEGRFLLKKLTSAVSLLQRESIPLLIVEKDSYSHPS
                     FAPAFEDLKSIASIQWRLTDTDGIFSVRIEQLPMRQASIIHKPVSIPSTNLQINSA"
     gene            complement(3175..5031)
                     /locus_tag="PKK76_08225"
     CDS             complement(3175..5031)
                     /locus_tag="PKK76_08225"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HNL68498.1"
                     /translation="MGDTFDFEFIRHLVKQAFHILNHEYFRAKLVNLDRVPAKTPGRP
                     RIFFSNHSGMAFPWDAIVFNTLFWESRGLANEDLPRPLVAPMLLESKLMSPFMIENLW
                     QRGGCLAASMENFEILMHEGKDVVMYPEGVPGIGKGFDKRYQIQKFSTSFLRMATEFN
                     AELIPFHTVNAEYLHPYSYKSDRLNRFVQKLGVPFLPLSPLTALVPLFPWMFYFSLPA
                     NLHFVFADPIQPSDLTSKSHQELKRSDYIRMRDDIHSDFQKGINIHVEEFGQDPFRLG
                     EFMEQLLSNPERALFTLPTGWPILITGLQKLYDDGLKKELKYTMDEFISLAARATDGL
                     SFSLPMSWPVVFGLKGFSVDIMRQIGHFMEEELAMATPEPLPEKPTPAEPSAKEAATA
                     ENLNEEASYAEAIQLLDDFEANAKNLDRATNILLPLTRGKNPGRAFGLLCQAQYWRGM
                     EATANKISIYEKGIEFGEAGVERDPGSVESHFWLAVNYGLLGETRGILESLFLVKPIE
                     AHLHKAIELDESYFFGGPHRALGWVLHKLPPWPLSHGSNQEALEHMERAIELGANFPI
                     NLLYAAHICVSLKDKKKAREYCDQLLGMEINPRHSREETRYRQEALAMIAKI"
     gene            complement(5087..5875)
                     /gene="folP"
                     /locus_tag="PKK76_08230"
     CDS             complement(5087..5875)
                     /gene="folP"
                     /locus_tag="PKK76_08230"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNL68499.1"
                     /translation="MRILGIVNITDDSFSDGGKFLSSRSAIDHAVHLSRGGADIVDLG
                     AASSNPEASRVPPAVEIERLAPVIRALRKGSVEVSVDSFSTEVQRFALSEGVHMLNDI
                     HGFADPDLYKELGKSSAMLVVMHSVQKEGVATKANTPAQEIVGRILNFFDERVETLLR
                     FVSRDRLILDPGLGFFLGTDPACSFAAIRAIPEIKSRYGLPVLISASRKSFLGAVTGR
                     AVLERSAATLACELEARRLGADYIRTHDPAALLDGCKIQDQIYS"
     gene            5937..6326
                     /locus_tag="PKK76_08235"
     CDS             5937..6326
                     /locus_tag="PKK76_08235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNL68500.1"
                     /translation="MSAFSSILNGTEPCEKILEDERYLAFMEKKPLKEGHVVVLPKRE
                     TDFIFDLSAEELSGLFVFSQRVAHAIRRAIPCKKVGVAVIGIEVRHAHVHLVPLDSGA
                     ELNFALPRREFTPDQFRVTADKIRAQI"
     gene            6367..>6599
                     /locus_tag="PKK76_08240"
     CDS             6367..>6599
                     /locus_tag="PKK76_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002721729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNL68501.1"
                     /translation="MILMPVSSTDFDPSEASIAWKVLNDRGIAVTFATPDGKPGSADH
                     RMVTGDGLGVWKRILMARKDAVAAYHEMTASSA"
BASE COUNT         1593 a         1502 c         1705 g         1799 t
ORIGIN      
        1 attcttctga gagatatctt tttcttctgt ctggatgttt agtattaagt actacgtatc
       61 gtactttttc acctgcctga accttgagtg atagatcgga caactgttcc atggccagat
      121 aggtggcgtt tttgacctga tagtctttaa attcacggct ggttgtcttt ctcagaagaa
      181 gctgctccca gggaacggtt ccatttctga gttcgctgca ttttcggtcg tataattcca
      241 tgacttctga atgaaggctg cgcagatgtt caatggaagt gccctttgcc attatttcta
      301 gtaattcgag ctgcgcgttt ctgataaaag aagccgtatc ttttcttctc gctgcaattc
      361 cacggaattt tatgcttccg tccagaaatc ggccacaata ccggttgacc actggaagca
      421 atctgtcctg acgagaagga agaaaaatca cccaggaata gatgccgtca agagacatct
      481 caattccgga agcattcgtg atggattctg ccatgcgaag aatagattct ttgtccagaa
      541 cctggccctg tctgtggagg aacacgcagt cagtgattgc atgagacaat gtatatcctt
      601 cttcttcagc gatttctttg gcaagcagta gcttctcgcg gccaaaggct gtgacgcttt
      661 cgtggctttc aattctgcca aactttgcat tgcgatagcc cagatatcca aaagaagtaa
      721 cgagcatcca tttgaggctg tcctgtcttg catcgtaaaa atcgatttga gaaatcgtat
      781 gttgcgtctc cggttgcgcc ttcagttcgc ggagtcttgt tttgtagtat ttgcgtctaa
      841 aaagaatgtg agcgagagcg tcagaaacca ctccgcgtct tcttttacat atatgatatc
      901 ctgcttccgg tacttttgga gcggaagggt cattggcaca gcacggacag ttcacgcatt
      961 caggcgaaat gttgtgattt accatgatgc ttggatacat ctgactgtaa tcgagctggg
     1021 ctacattttc aaacacgctt gtttcagatg cgttgggcac aaagatcaat ccgcctttgt
     1081 ctacagccaa tagatcgtat gctgtttttg gcttttctac tgcacttttc tgccacggca
     1141 ccagatagtg ttttcggatg gctacgtgag tttcaatgga tgtaagtgcg ccgcccgtgg
     1201 attgccgtga cattctctgt atgggtgtgc gcgccagtct tgccagttcg aatattccaa
     1261 ccaggcgcga ctctttgaat acgaaggaat tgtgtgcgtc cacatgaagg cggccgaata
     1321 gaggatagga ggcagcgcga aagatccagc tgccatacgt attgaagctg gtcccttcgc
     1381 gtacgatttt acgcagactg tgatgaccat gtcgatcgaa tagtagaggg atattgtgag
     1441 tttgtgcaag agtgaatagt tcaggaaata tgatttgatc tccataagaa gaaacaatga
     1501 tatccggatc ttcctcggcg agtatgcgat tgatctggag caggcattct ttctggttgt
     1561 gagcggagag agcaatggtc ccacggcttg tttcgaaaat gagacgattg tctttcagcc
     1621 ccagtctgtg gctcttttcc agctttattc cgatttgttt gaacggtgga atatcaaatt
     1681 ccagatctgt gatgcgatcc agggatacaa ttcgctcgat gttcctggtc tggcctgaag
     1741 tagtatagtc gattcgaacg cgcgaaagtg gatgtatgtt cttgtgaaac atgtatccaa
     1801 ctggaatttc aatgtctgtg tgatatagat ccagtttgcc gtagaaggag taaaggcgtc
     1861 ttcgcacgcg gttgagaatg gcgggtttgg caattgtgat ttttaaaact cttctgggcg
     1921 agttatcgta gaagtgattt cggatggtcc agactggctg gccatggatg gctcccagct
     1981 catggaatct tgtgactagc ttttttaaaa gatcaaccgg gccgtctgcg aatatgattg
     2041 gatggaagga atcgatgaag tggagtgccc tggaatctgt tttgatccag agatgaattt
     2101 catcgttgaa attgtataca tccagaagtg tgccttcaac tgtttccatc ctctttctcc
     2161 gtgtgcagaa gggactggtt tttgatttca agtacgacgc tttgcaatgc tttgatttgt
     2221 tctgattgaa cgtcgatttg tctttggatt tctataagca tggtgagtgc cattgtatca
     2281 aaaggattcg ggctggacgc catggctccc gcctggatct gcatgcgagc cgttctcatg
     2341 agccggtcga agatcttttg atcttctttt ctgaggccac gtcttagctt tttgagtcga
     2401 tcttctacaa gatcgatctg catggaatag ggaaggtgcg ttcgtcccat acgttatgcg
     2461 gaattgatct ggagatttgt ggacggaatg gagactggtt tgtgaatgat ggatgcctgt
     2521 ctcatgggga gctgttcaat tcttactgaa aagattccat ctgtatccgt gagccgccat
     2581 tgaatggatg caatggattt taaatcttcg aaggcgggag cgaaagaagg atgcgagtac
     2641 gaatcttttt ccacgatcag aagaggaatg gactcccttt gcagaaggga aacggcgctc
     2701 gtgagtttct ttagaaggaa tcgtccttcc tcatcagaca catctccgtc gaagaactgc
     2761 ttaaacggag ccagcaagaa gtaagtgctg tccggtggtt tgtgtgctgc gtccaggatt
     2821 ccatacagca cgtccaggat ctggtatgga gtgaatgctc tctgtacgag tatggattga
     2881 agcagcgctt ctgtttgcag tcccatgcgc atggcttcat ccgccagata aaacgcgttg
     2941 aatcgaatgg cgcagtcaat gaccaggact ctctggccaa gaaggctgag gtgggtttgc
     3001 cacagatggg atagatgaaa gattccgctt cttccagtca aaaggcctgt tttgcctgaa
     3061 aaagcagtga aatagcgcaa tgaatccgtg gggcgaacca gcatgatcca tactagtgtc
     3121 ggctatacaa atgtcaagta ttagcctttc ttttcgcaac ggagctctgt ttgctcaaat
     3181 tttcgcaatc atcgcaagcg cttcctgtct gtacctggtt tcttccctgc tgtgacgagg
     3241 gttgatttcc atacccagca attgatcgca gtattctctg gcctttttct tatctttcag
     3301 agatacgcag atgtgcgcgg catacagtag gttgattgga aaattcgcgc caagctcaat
     3361 ggcgcgttcc atgtgctcga gtgcttcttg attgctacca tgcgaaagag gccatggagg
     3421 tagtttgtgt agaacccatc caagagcgcg atgagggccg ccaaaaaaat aggattcgtc
     3481 cagttcgatg gctttgtgca gatgcgcttc gattggtttg accagaaaca gactttccag
     3541 aatgcctctc gtttcgccta acagaccgta gttgactgcc agccagaaat gagattctac
     3601 gcttccagga tctctttcca cccccgcctc accaaactcg attccctttt cgtaaataga
     3661 gatcttgttt gccgtggctt ccattcctct ccagtattga gcctgacata gaagtccaaa
     3721 tgcccgaccc ggattttttc ccctggtcag aggtagaaga atgtttgtgg ctctgtccag
     3781 gtttttggcg ttcgcttcaa agtcgtccag gagttgaatc gcttccgcgt aggaagcctc
     3841 ttcatttaga ttctcagcag tcgcagcctc tttcgcagat ggttccgcag gtgtgggctt
     3901 ttccggcaga ggctccggtg ttgccatggc cagttcctct tccatgaaat gaccaatctg
     3961 ccgcatgata tcgacagaaa atcctttcag gccgaataca acaggccagg acatggggag
     4021 agaaaaggac aggccgtccg tggcacgggc tgccagggaa ataaattcat ccatcgtata
     4081 tttgagttct tttttcaggc catcgtcata gagtttttgc agacccgtga tgagaatcgg
     4141 ccaaccggtt ggaagagtga agagagctct ttcggggttg gatagaagct gttccatgaa
     4201 ctcgcccagg cgaaacggat cctgaccgaa ctcttctacg tgtatgttga ttcccttctg
     4261 aaaatcagaa tgaatgtcgt ctctcatgcg aatataatcg cttcttttca gttcctggtg
     4321 cgatttcgag gttagatcgg atggctggat cgggtctgca aaaacgaaat gaagattcgc
     4381 gggcagggag aagtagaaca tccagggaaa aagaggaaca agggcagtta acggggacag
     4441 aggcagaaac ggaaccccca gcttctgcac gaaacgattc aatcgatcgc ttttgtagga
     4501 atacggatgc aagtactctg cattcacagt gtgaaatgga atcaactctg cgttgaattc
     4561 agtagccatg cgaagaaaag acgtcgagaa tttctggatc tggtatcgct tatcaaatcc
     4621 ctttccgatt ccagggactc cttctggata catcactacg tctttgcctt catgcatcag
     4681 gatctcgaaa ttttccatac tggccgcgag gcacccgcct ctctgccaga ggttttctat
     4741 catgaaagga gacatgagtt tgctttccag aagcatgggc gcaaccagtg ggcgagggag
     4801 atcttcattg gcaagcccgc gcgattccca gaacaaagta ttgaatacaa ttgcatccca
     4861 cggaaatgcc attccggaat gatttgaaaa aaagattctg ggtctacccg gcgttttcgc
     4921 aggcacccga tccaggttca ccagttttgc gcgaaagtac tcatgattga gaatatgaaa
     4981 cgcctgcttg accaggtgcc ggatgaattc gaaatcgaaa gtgtccccca taacacacga
     5041 agactaacac agttgcacgg gggatggtat gacaaatgtc agaaagctag gaataaattt
     5101 gatcctgtat tttgcagcca tccaggagtg ctgctgggtc atgcgttcgt atgtagtctg
     5161 cacccaggcg cctggcttcc aactcgcagg caagagtggc cgcggatctt tccaggactg
     5221 ctctacctgt aactgcaccc agaaatgatt tcctggaagc ggaaatgagt acaggcagtc
     5281 cgtacctgga tttgatttca gggatggcgc gaatcgcagc gaagctacag gcggggtctg
     5341 ttccaagaaa gaatccaagc ccggggtcca ggatgagcct gtctcgtgat acgaaacgaa
     5401 gaagtgtttc tactctttcg tcgaagaaat ttaaaattcg tcctacgatt tcctgggccg
     5461 gagtgtttgc ttttgtagcc acgccttcct tttgcactga gtgcatgact acgagcatcg
     5521 cacttgattt tccaagctct ttgtagaggt cagggtccgc gaagccatgg atgtcattga
     5581 gcatgtgcac tccttccgac agcgcaaatc tctgcacttc tgtagagaac gagtctacag
     5641 acacttccac ggagcctttt ctcaaagctc gaatgaccgg tgccagtctc tctatttcta
     5701 cggcaggtgg aacacgggaa gcttcggggt tgctcgatgc cgcgcccaga tcgacgatgt
     5761 ctgcgccgcc gcgcgacagg tgcaccgcgt gatctatagc agatcggctg gatagaaatt
     5821 tgccaccgtc agagaaagaa tcgtcggtta tgtttactat gcccaggatt cgcacgctcg
     5881 attttcaccg ggcgttgtgt gcttgcaagc ccgatcggaa ggtctcgaat gacgcaatga
     5941 gcgccttttc atccattctg aacggcacag agccctgcga gaagatcctg gaagatgaac
     6001 gttatctggc attcatggaa aagaaacccc tcaaagaagg ccatgtggta gtgctgccaa
     6061 agagagagac tgactttata tttgatcttt ctgcagaaga actgagcggg ctgttcgttt
     6121 tctcacagcg cgtggctcat gcgattcgtc gtgcgattcc ctgtaagaaa gtaggcgttg
     6181 cagtcattgg gatagaagtg aggcatgcgc atgttcacct ggttcctctg gattcaggtg
     6241 cggagttgaa tttcgcgctt cccaggcgcg aatttactcc ggatcagttt cgagtgaccg
     6301 cagacaaaat ccgcgcacaa atttgaaaat cgcatgactg atcgaagagg ggggcctcat
     6361 tgaggcatga ttctcatgcc agtgtccagc accgattttg acccttctga agcaagcatt
     6421 gcctggaaag tgctcaacga tcgcggaatc gcagttacat tcgccactcc agacggcaag
     6481 ccgggaagtg cagatcatcg catggtcaca ggggatgggc ttggagtatg gaagcggatt
     6541 ctgatggctc gcaaagatgc tgtggcagcc tatcatgaaa tgacggcgtc ctccgcctt
//
LOCUS       DAORFF010000166         6595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6020_length_6595_cov_3.834709, whole genome shotgun sequence.
ACCESSION   DAORFF010000166 DAORFF010000000
VERSION     DAORFF010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6020_length_6595_cov_3.834709"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PKK76_08245"
     CDS             complement(<1..887)
                     /locus_tag="PKK76_08245"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNL68502.1"
                     /translation="MDPIFLIAGLGTNQDLQLSTSMSRAGVTPLAPAPPQNAALVTLG
                     RALFFDKELSGNRNIACSTCHLRNSGTSDGLSLSIGWGGNGLGAVRTVGTGNFVGRNA
                     PPLFNLGAKITKYFWDGRVSRADNGILTTPEPALNGASPTRADIASQLTTGLAAQAMF
                     PVTSDLEMRGLPGNPVRDAANNQAVWAVLMSRLVGTNNGTVGGLPGYRALFAAAFPTV
                     TNFDNFNFGHAAAAIAAFETESYSAVGSPFEQYVKGNTGALTPEAKAGGILFFGKANC
                     STCHNGPMLTDNEFHGVASP"
     gene            complement(1159..1599)
                     /locus_tag="PKK76_08250"
     CDS             complement(1159..1599)
                     /locus_tag="PKK76_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNL68503.1"
                     /translation="MDNSSITFETIRDPDGDLQEFLWERLREASNPKLSDERAKEHHA
                     LSILAREKGEIVGGMLAIVYFRGMNLQCLWVREDQRGKKLGSAFLEQAEQAARSLDCT
                     LIHGHTFSFQAPDFYLKAGYEVFGTIEDYPPGKNCFFLRKYLRK"
     gene            complement(1592..2485)
                     /locus_tag="PKK76_08255"
     CDS             complement(1592..2485)
                     /locus_tag="PKK76_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNL68504.1"
                     /translation="MSQNGKVRTIARIIDSIRQREGGGFIVRRPFPTQSVSYVDPFLL
                     LDEMGPADYAPGEAVGAPDHPHRGFETVTYMLEGEFQHEDSAGHRGFLRPGDVQWMTA
                     GAGIIHSEEPSEKIMREGGRVHGFQIWVNLPSQLKMTQPRYQEVPGSGIPTAQTEDGL
                     ARVRVIAGEALGVRAVIETHIPIVYQDWTLSPGASASVIVPQEQNAMIYVFEGSVAVG
                     DRQNVLEDGQLGVLGLGDTVQLFGTQTGGRMLLLAGVPTSEPVARYGPFVMNTVEELQ
                     TAFNDYQSGKMGEITRGAKYG"
     gene            2595..3398
                     /gene="modA"
                     /locus_tag="PKK76_08260"
     CDS             2595..3398
                     /gene="modA"
                     /locus_tag="PKK76_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNL68505.1"
                     /translation="MAIAARIAVQNSKLSLMLDPAHFRTRNLSFILKQPARNRVCVLI
                     WVLSCVFISCSPTGPRPVTIFAAASLKETISELSNEFEKSGRKTQVQFDASSTLARQI
                     QQGALADVFITAAPEWLESMRILDRFDWLSNRLVIVVPLNDKSSFRLRNMKSLALANE
                     QVPAGKYARAAFAHLHIPIPERTIYGSSVRDVLFIVAKGGAEAGVVYATDAATAPELG
                     VAFTFPEESHPRIVYSVGLLRPEGKEFYDLLQARPEAATKHGFIDIRKQ"
     gene            3395..4066
                     /gene="modB"
                     /locus_tag="PKK76_08265"
     CDS             3395..4066
                     /gene="modB"
                     /locus_tag="PKK76_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNL68506.1"
                     /translation="MNSIQLSLSVGFAATLLTLPLAILAAWFLEKTDSKARPILQILI
                     TLPLVLPPVVIGYLLLALFSPSGKVGSTLQWLGAPVAFHWLGAVVSAAVVGFPLFVTS
                     ISLSMREIDARLERISASLGASRLQTFFRITLPLAWPGILSGAALAFARGLGEFGATI
                     ILAGRIPGQTETIPLAIYAELDSPGGETKIAWLVFASIAICVILILVSRIFQGLHKRK
                     MESES"
     gene            4063..5061
                     /locus_tag="PKK76_08270"
     CDS             4063..5061
                     /locus_tag="PKK76_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNL68507.1"
                     /translation="MILEIEANVSRPNGFHLIVEEKWSAHATGIAGPSGGGKTTLLNC
                     ITGSERGARVIVDGEDWSEWSMQERKIGYVTQDPLLFPHMTVKENLLYSPRASSIGPI
                     AEMLRIDHLLERRPANLSGGEKRRVALGRAIASQPRLLVLDEPFTGLDEILRREAMSV
                     LHSIKQWSGVPMILVSHLADEIVGLTDWTIRLENGSVSKRGPSESVLNETETRIDNYF
                     SGIILEPGLVRCGPCDLKVILPHDADGPVRLACYANDILLSKKPPDEISARNVIPTKI
                     KLIHGFEKITLVDLEAPSLRVLVTREANRDLDLVTGMSIYAIIKASSIVYLGPAEN"
     gene            complement(5058..5444)
                     /locus_tag="PKK76_08275"
     CDS             complement(5058..5444)
                     /locus_tag="PKK76_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5360 family protein"
                     /protein_id="HNL68508.1"
                     /translation="MRFLPFFFLVTDIGFILYWVITLIGIIPADYLFKDYNNPILSAW
                     NWSFLPLDLFISFSGLYSLRLRAKGFPWKNVALISLVLTLCSGLQAVSFWAIRRDFDL
                     AWWLPNLFLFLYPIYFIAAILRGSRD"
     gene            5539..6045
                     /locus_tag="PKK76_08280"
     CDS             5539..6045
                     /locus_tag="PKK76_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNL68509.1"
                     /translation="MKYLLFAFLLISASLLAEEEKLATLETACQEGEVEKVKSILKGI
                     PGAEQNQKAKGCLATAVSEAHIPLVRFLVERKTPLDHHYGWTPLHRAAKLSTPDHLKI
                     ARILVDAGARVDVGLKSPDGEPYMGPYGIGMSPLGVARKSRDKFTDEKARAPFDAMIA
                     FLKSRSGQ"
     gene            complement(6124..6549)
                     /locus_tag="PKK76_08285"
     CDS             complement(6124..6549)
                     /locus_tag="PKK76_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68510.1"
                     /translation="MKRLSTIFTIFIFFVGAVLPGCKKEEKDHTSEYALIVLFAASFT
                     RTFSFSCNKFSVNSSCTNYYGFGTSSATCTSGGGTVSTSKCSNLSNYGTCTTAAGGSG
                     SSEFVFYSGGTNPVCGSAGACQTNCTAVGSGTYSSTYTP"
BASE COUNT         1672 a         1717 c         1661 g         1545 t
ORIGIN      
        1 ggagaggcaa ctccgtgaaa ctcattgtcc gtcagcatgg gtccattgtg acacgtggag
       61 cagtttgctt ttccgaaaaa caaaatcccg cctgcttttg cctctggtgt gagagcgcca
      121 gtatttcctt tcacgtattg ctcgaagggt gagcccactg cagaataact ctccgtttca
      181 aaggcagcaa ttgctgctgc ggcatgtccg aagttgaaat tatcgaagtt ggtaacagta
      241 ggaaaagcgg cagcaaaaag agcgcgataa ccgggcagtc cacctacggt accgttattc
      301 gttccaacga ggcgagacat gagaacagcc cagaccgcct gattgttggc cgcatctctg
      361 accgggtttc ctggaagacc tcgcatctca agatcggacg taactggaaa cattgcctgt
      421 gcggccagac cggtcgtgag ttgacttgca atgtctgcgc gcgtcggaga tgcaccgttg
      481 agggctggct cgggtgtggt aagtataccg ttatccgctc tactcacacg cccatcccag
      541 aagtatttcg taatcttcgc acccaggtta aacaaaggcg gagcatttct tccgacaaag
      601 tttcctgttc caacggttcg cactgcacca agtccgtttc caccccatcc gatcgacaag
      661 gagagaccgt ctgatgtgcc cgagttgcga agatgacagg tagagcaagc gatgttgcga
      721 tttcctgaaa gttctttatc aaagaaaaga gcgcggccca gagtcacaag agccgcgttc
      781 tgagggggcg cgggcgccag aggagtcaca cctgccctgc tcatgctagt gctcaattga
      841 aggtcttgat ttgtgccaag ccccgcaatc aagaagatgg gatccaccgc gggccccccg
      901 tgcgattgtt gggaacaaga cgccgaaaag accgcaatca gaacgaacat ccatgtgcaa
      961 agtgagcgtg ccatacgtga ttcctctaac aaacaaccag acatgtccag cagtttgcgt
     1021 acgaaacctt cgcgaacgtt aggcgatcgt caggggcgcg acagagaata cgtgaacacc
     1081 atcttttcag tcatgaacaa cagaaatcga tagccgaaat ctgccatcgt gtgctgttca
     1141 tgactggttt tgtgtcgttc attttcgtaa gtattttcgt aaaaaaaagc agtttttacc
     1201 gggtggatag tcttcaatcg tcccgaaaac ttcatagcct gccttcagat agaagtcagg
     1261 tgcctggaag ctgaaggtat gcccgtgaat caaagtgcag tccagggatc tcgccgcctg
     1321 ctcggcttgc tccaggaatg cggaaccgag cttcttacca cgctgatcct cgcgtaccca
     1381 gagacattgc aggttcatcc cacgaaagta aacaattgca agcattccgc cgacgatctc
     1441 tcccttttca cgagccagta tgctcagcgc atggtgttcc tttgctcttt cgtctgaaag
     1501 ctttggattg cttgcttccc gcaggcgttc ccatagaaat tcctggaggt ctccgtctgg
     1561 atctcgaatc gtttcgaatg tgatcgatga attatccata ttttgcgccg cgagtgatct
     1621 ctcccatttt gccgctctgg taatcgttga atgctgtctg caattcttcc acggtattca
     1681 tgacgaaagg gccgtacctt gcgacgggct cgctcgtagg aacacccgca aggagaagca
     1741 tgcgcccacc tgtttgcgtt ccaaacaatt gaactgtgtc tcccagccca agaacaccaa
     1801 gttgtccgtc ttcgagcaca ttctggcgat cgcctacggc aacgcttcct tcgaaaacgt
     1861 agatcatcgc attttgctct tgcggaacaa tgacagacgc agatgcgccg ggtgaaaggg
     1921 tccaatcctg atacacgatg ggaatgtgag tttcgatgac tgcgcgaacg ccgagtgcct
     1981 cacctgcgat cactcgcacg cgggcgagac catcttctgt ttgcgcagtt ggaatccccg
     2041 atcccggaac ttcctggtaa cgcggctgag tcatcttcag ttgagagggc agattcaccc
     2101 agatttgaaa cccatgcact ctgccgcctt cacgcatgat tttctccgaa ggttcctctg
     2161 aatgaatgat tcccgcacca gcagtcatcc actgcacgtc cccgggccga agaaaaccgc
     2221 gatggcctgc agaatcttcg tgctggaatt cgccttcgag catgtaggtg accgtttcaa
     2281 atccacgatg cggatgatcc ggagctccca ctgcttcccc gggtgcatag tccgccgggc
     2341 ccatctcatc tagaagtaga aacggatcta cataactgac agactgagtt ggaaacgggc
     2401 ggcgaacgat aaacccgcct ccctcgcgct gtcgaatcga atcgatgatc cgcgcaattg
     2461 tacgaacttt tccattctgg ctcatagtgg acctctcatt tataagttct ctttaatatt
     2521 gaaggttcaa ttactttcaa tggccattct tttttacccg gagctcaaaa tgttcagcca
     2581 ggcaaaagag ttgcatggcg attgccgcgc gcattgctgt gcagaactca aagctttccc
     2641 tgatgctaga tccagcccat ttcagaacgc gcaatctatc gttcattctc aagcaacctg
     2701 cacgcaatcg tgtctgtgtc cttatctggg tcctctcgtg cgttttcatt tcttgcagtc
     2761 ctactggtcc gcggccagtc actatttttg cagcggccag cctgaaagaa accatcagcg
     2821 aactttccaa tgaatttgag aagagtggaa gaaaaacgca agttcaattc gatgcatcct
     2881 caacccttgc gcgacagatt caacagggag cgctcgcgga tgtattcatc actgccgctc
     2941 cagaatggct cgaaagcatg aggattctgg atcgatttga ctggctttcc aatcgtcttg
     3001 taattgttgt tcccttgaat gacaaatcct ctttccgcct ccgcaacatg aagagtctgg
     3061 ctcttgccaa cgaacaggtc ccggcaggaa agtacgcgcg cgcagcgttt gctcaccttc
     3121 acataccgat tccagaaagg accatctacg gttcaagcgt gcgcgatgtg ctttttattg
     3181 ttgcaaaagg tggcgcggaa gcaggcgtcg tgtatgcaac ggacgctgca accgcaccag
     3241 agcttggcgt ggccttcacg ttccctgaag aaagtcatcc tcgcatcgtg tactccgtcg
     3301 gtttgctgcg gccggaagga aaagaattct acgacctact gcaagcgcgc ccggaagcgg
     3361 ctacaaaaca cggattcata gacattcgaa aacaatgaac agcatccaac tatcactctc
     3421 cgtcggattc gcggcaaccc tccttacact tcctcttgcc atcttagctg cgtggtttct
     3481 ggaaaagacg gattcaaaag ccagacccat tttgcagatt ctaatcaccc taccacttgt
     3541 gctccctccc gtcgtgattg gctatctgct actggctttg ttttctccct cgggaaaagt
     3601 cggctccaca ttacagtggc taggtgctcc ggtagcgttt cactggttgg gcgcagttgt
     3661 ttccgcagcc gtagtcgggt ttcccttatt tgtaacgtcc atctctcttt ccatgagaga
     3721 aatcgatgcg cgtctggagc ggatttctgc atctctggga gcttcacgcc tgcaaacttt
     3781 cttcagaatc acgcttcctc tcgcctggcc aggaatacta tcaggcgccg cacttgcatt
     3841 cgcacgcgga ctcggagaat ttggagcaac catcatcctg gcgggtagaa tccccgggca
     3901 gacagaaacc attccgcttg ccatctatgc tgaactggat tcgccgggcg gagaaactaa
     3961 aatcgcctgg cttgtcttcg catccattgc gatctgcgta atcttgattc ttgtatctag
     4021 aatcttccag ggcctgcaca aaaggaaaat ggaatcagaa tcatgattct ggaaatcgaa
     4081 gcgaatgtct ctcgcccaaa tggttttcat ttaatcgtag aagaaaagtg gagcgctcat
     4141 gcaaccggca ttgcggggcc gtcaggcggc ggaaagacca ctctgcttaa ctgcatcacc
     4201 gggagcgaaa gaggggccag agtcatagtg gatggagaag actggtccga atggtccatg
     4261 caggagcgca agattggata tgttacgcag gatcctcttt tatttccaca catgactgtg
     4321 aaagagaatc tactctatag cccgcgcgca agttccattg gtccgattgc agaaatgctg
     4381 cgcatagatc atcttctgga acgcaggcca gcaaatctga gcggaggaga aaaacgacgt
     4441 gtggccctgg ggcgagcgat cgccagccag ccccgcttgc ttgttctaga tgagccattc
     4501 acgggactcg acgaaattct acgacgcgaa gcaatgtcag tactacatag catcaaacaa
     4561 tggtccggcg ttcccatgat tctagtaagc catctcgctg atgagattgt agggctgacg
     4621 gactggacga ttcgtttgga gaatggttcc gtctcgaagc gcggtccgtc cgaatccgtg
     4681 ctgaatgaaa cagaaaccag aatagacaat tacttttccg ggattatttt agagccgggt
     4741 ctggttcgct gcggtccatg cgatctaaaa gtgattctcc cacatgacgc ggatggtccg
     4801 gtcaggctcg cctgttacgc aaacgacatt cttctttcta agaaaccgcc tgacgaaata
     4861 tcagcgcgaa atgtgattcc aaccaaaatc aaattgattc atggctttga aaaaatcacg
     4921 ttagtcgacc ttgaggcgcc gtcgttgcga gttctggtga ccagagaagc aaatcgagat
     4981 ctggatctgg ttactggaat gagcatttac gcaatcatca aggcaagttc cattgtctat
     5041 ctgggtccgg cagagaacta gtcccggctg ccacgcaaga ttgcggcaat gaagtagatc
     5101 gggtagagaa acaaaaacag attcggtagc caccaggcca gatcgaaatc gcgacgaatc
     5161 gcccagaaag acactgcctg caggccggag cacaacgtca gcaccagaga tatgagtgcc
     5221 acgttcttcc agggaaaccc ctttgcgcga aggcgaagac tgtagagccc ggaaaaagaa
     5281 atgaacagat ccagaggaag aaacgaccaa ttccaggcgc tgaggattgg attgttataa
     5341 tccttgaata aataatccgc aggaatgatt ccgatcagag tgatcaccca gtagagaatg
     5401 aacccaatgt ctgtgaccag gaagaaaaag ggaaggaagc gcatgattgt ttcaagttgg
     5461 agaaagacgg cgcggggcac aagcccaaag aagagtggat ggaagttgac atttcctgca
     5521 gtgcggaaag gattcggcat gaaatatttg ttattcgcgt ttctactcat ctctgcctct
     5581 cttcttgcgg aagaggaaaa actggctacc ctggaaacag cgtgccagga gggagaggtc
     5641 gaaaaggtaa aatccatttt gaaaggaatt cccggggcgg aacagaacca aaaagcgaaa
     5701 ggatgtcttg ctaccgcagt gtcagaagcg cacattcctc tggttcgttt cctcgtggag
     5761 agaaagacac ctctggacca ccactacgga tggactcctc tgcatcgcgc ggcaaagctc
     5821 tcaacaccgg atcatttgaa gatcgcacgt atcctggtgg atgcgggtgc acgagtggac
     5881 gtcggactca agagtccgga cggagaacct tacatgggcc catatggaat tggaatgtca
     5941 ccactcggtg ttgcgcgcaa aagcagagac aagtttacag acgagaaagc acgtgcaccg
     6001 tttgatgcga tgattgcgtt tctcaaatcg cgcagcgggc agtgatagaa tttcgcgcat
     6061 cagcaaattc ttccaaacca cggcgcgaaa ttcttcagcg gacagatacc aaacgcgaag
     6121 cgatcaagga gtgtaagttg aagagtatgt accggaaccg acggctgtgc agtttgtctg
     6181 acaggcgcct gcgctaccgc atacagggtt cgtgccgccg gaatagaaca caaattcaga
     6241 ggagccagaa ccgccagcgg cagtcgtgca cgttccgtag ttcgatagat tcgaacattt
     6301 ggaagtggaa actgtgcctc caccggaggt acacgttgca gaactcgtgc caaatccgta
     6361 atagttcgtg caactactgt tcacgctgaa tttattgcag ctgaatgaaa aagtacgagt
     6421 gaagcttgcc gcaaacaata cgatcaacgc gtattcagat gtatgatctt tctcctcctt
     6481 tttgcagcct ggtagaacgg ctccaacaaa aaagatgaaa atagtaaaaa tagtagacaa
     6541 tctcttcatg gccttctgta cccccccccc ccccgaattt tcaactattt atccg
//
LOCUS       DAORFF010000167         6592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6028_length_6592_cov_3.404620, whole genome shotgun sequence.
ACCESSION   DAORFF010000167 DAORFF010000000
VERSION     DAORFF010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6028_length_6592_cov_3.404620"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..1254)
                     /locus_tag="PKK76_08290"
     CDS             complement(262..1254)
                     /locus_tag="PKK76_08290"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNL68511.1"
                     /translation="MVLSILVLLVASIFILGHFGLPEPARPMPEPIDPRQIMPGAEPY
                     RFEGTSGVAFLICHGFEDSPLTTRPLGEFLHGLGHTSIGILLPGHGTKIEDLTRVRFY
                     HWQDYLETLFREERSKYRKVFLVGFSMGGTLMLDLAGRFADTYRPAGVLTISSPVFFN
                     GFFNGKLIIHQPMTMFTGIMRIFTSILRIEGKKKTNSMQRLNPWLGYDATYALDTLHS
                     FKRSLSGVRRVLPRISVPYCSIMAANDRTVSAENQIYIYNNIGSREKRALQFILPPDM
                     TNMHTLLTHKKAQKRVLRFIQEFVDDTLKDFVPETPRRGFFARLFRRTEQSVEN"
     gene            1341..1559
                     /locus_tag="PKK76_08295"
     CDS             1341..1559
                     /locus_tag="PKK76_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNL68512.1"
                     /translation="MAELTRRLQILLDPDQMESLQAIAQKRRKSVAELIREAVDIQYR
                     PRTEVRRIQAIESLRSFSSIHISTDDLK"
     gene            1586..1984
                     /locus_tag="PKK76_08300"
     CDS             1586..1984
                     /locus_tag="PKK76_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68513.1"
                     /translation="MEKWFLSFDAILVLASDLPAKSALLDQLSEFLKSGILVTSSHSV
                     QRCAEFFEHRGEKSFRSFYSRLRPALDHVLEVTERDQSYALDLAESSNISLALALEVA
                     VAIHSGASGFVSSNSKIDSLSELPRIAVAI"
     gene            1981..3147
                     /locus_tag="PKK76_08305"
     CDS             1981..3147
                     /locus_tag="PKK76_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNL68514.1"
                     /translation="MSQEQVVELTEEESSTQELARRISNQDLAPMADELDRHEQFAHP
                     VFQKLGEAGLLGIVIPEEFGGADGSFLSAVLIMEELARNCGGTALSFGAHAFIGAYNL
                     FKFASDSQRKKYLPDLCSGKKYAAFALTEPENGSDAMGLRTRAEKRGASYILNGAKTF
                     ITNGSVADTFVVFARTGDAGAKGISAFIVERSFPGFASGKPMEKMGMRASPTTELYFE
                     NCEVPAENLLGAEGQGGYFALEGLDMERTLFSGLPIGLIQGAFDVALKYSTERSQFGS
                     KIAGLQLIQAMVADIGMNLFVSRLLAYTAARKLQEKKRVTLNASFSKLFASEASVKST
                     LDCIQMLGGYGYIREFKVERLMRDAKLVEIGGGTSQIQRLIIAREIYKSHDLSP"
     gene            3201..3542
                     /locus_tag="PKK76_08310"
     CDS             3201..3542
                     /locus_tag="PKK76_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68515.1"
                     /translation="MPDLNTDQSRGQVQNLPSASSGPRAIEPVVVPETELPPATGAQR
                     IFWIIIAILCAGWIFIPNFFIIPIPFFIDEGIAAIILMTALSKLGIRIPILHWFFEKR
                     MGKSIALKTKR"
     gene            complement(3543..4850)
                     /locus_tag="PKK76_08315"
     CDS             complement(3543..4850)
                     /locus_tag="PKK76_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNL68516.1"
                     /translation="MEDAKLILDNQEYHLPVVQGTDGSRSIHADSLFEKTGLRIFDPR
                     LNNVNITKSAITYIDPVSGRLLYRGYRIEDLVEHSSFVEVSFLLNEGELPNQEQYANY
                     SNELCKHSMIHESMRYFFDAFPGDAHPIAILATMVTALSAYYPSTYEENLKKGVDIRA
                     RLLAKVRTLAAWSYKKSVGEPVVYPRDEMPYCTNFLNMLFAVPAETYQVPKSHDRVLN
                     QLLILYSDHEQNTATTTMRLVASSQANLFACINAAMCAMWGSREFGANIPPIPMLDQM
                     TKRRQSPEEYFEKFIKGQEIIRSNGLGHSSYRTQDPRAIIARKLFHEFRKTNEIAAVD
                     PLFEKALEVEEFTLGHPYFKEKGLYPNLDYYSALIFRMIGLPMTMNNVIRTIGKLSGW
                     LAHWMEQHDEGVRGAYRPGQVYTGVAERPFPTDRQGTHWGRET"
     gene            complement(4905..>6592)
                     /locus_tag="PKK76_08320"
     CDS             complement(4905..>6592)
                     /locus_tag="PKK76_08320"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNL68517.1"
                     /translation="NGELIGEKSSEKYGYGRPRVYPLPASILRQGENTIAIRLEGSFK
                     DFMGITSGPVRIDTIDRAEATVWKTALKSLVLSGIYIATGIFFIVLFLRVGGMRDYFY
                     YSLFTISFALQQLMRNELRFQIADWFYAFKAAEQMLYIACATSFFFFFVSFFKLKLNR
                     LVYLYPAFNGVVALILPFLGSPVRIDAVMTIWFPLNLPFFAWYAYHACQRALRRDSDA
                     MILAGGMIIMLVATLLFFLSERGFIKWGGGVFDSSVFIFIFALSFILIFRLIRLHLDV
                     ERRRTRLDSVNALRDRVFRYIDGILRGPAQAIVNLTLAARNADLREKEKKELLDKIDT
                     DLGVLQAEMDDILELSRLEVIQEPESFENVNFKDFITAVIPQGAITSYINVDPDIEIK
                     TSLELVNSMVIRLIDFPGFREFKHIDLIITSDLSENIHFRFLLFHNDFRQTRKLHELI
                     TSLNPERGTLWAKWAIVREIIRILDGSMAISIINRKFLRIDIKLKSERVEVQKNVTGE
                     IRIVPIASAIAAEPAMQTAEAVPVPDSMPAPKLSGNMSLGEFAAYIKHKLKRK"
BASE COUNT         1618 a         1564 c         1727 g         1683 t
ORIGIN      
        1 ttctgcgtct acagtggtgg cagatcgaat gatgaggccg tgcacatttc ctatgatctt
       61 gagtagttcg tcgcgttttg ttccgccgga gtaaattact tctaaatccg catgggcttt
      121 cagtttggag acgccttctg cgtcgaattt gtcagcaatg agtatggtat gcttcatatg
      181 agccatgttt taggagggca cccttcgcta aaagtgattt agcggagcga aagccgcagt
      241 tttcgcacgg cccagagacg gtcaattttc gacggactgc tctgttctcc tgaaaaggcg
      301 agcaaagaag ccccgccggg gcgtctccgg gacaaagtct ttgagagtgt cgtcgacgaa
      361 ttcctggatg aaacgcagta ctctcttctg cgcttttttg tgggtcaaaa gagtatgcat
      421 gtttgtcata tcaggaggca gaatgaactg gagagcccgt ttttctctgc ttccaatgtt
      481 gttgtagatg tagatttgat tttccgcaga cacggttcga tcgtttgcag ccatgatgga
      541 gcaataagga acgctgatgc gcggtagaac tcttctcact ccggagagag atctcttgaa
      601 actatggaga gtatcgagcg cgtaggtggc gtcatatcca agccaggggt tcagtctctg
      661 catgctattg gttttctttt tcccttctat gcgaagaata gaagtaaaga tgcgcatgat
      721 tccagtaaac atggtcatgg gctggtggat gatgagtttt ccgttgaaaa acccattgaa
      781 gaaaacggga gatgaaatgg tcagcactcc agcggggcga tacgtatctg cgaatctgcc
      841 cgcaagatcg agcatcagtg tgccacccat agaaaaacca accaggaata cttttctgta
      901 tttgcttctc tcttctctga atagagtctc cagatagtct tgccagtgat agaatcttac
      961 gcgcgttaga tcttctattt ttgtcccgtg gcctggaaga agaatgccga tcgaagtatg
     1021 tccgagtcca tgcaggaatt ctcccagcgg tcgagtggtc agaggtgaat cttcaaatcc
     1081 gtggcagatg agaaaagcca ctccgcttgt gccttcgaat ctgtacggtt ccgctcctgg
     1141 catgatctgc ctcgggtcga ttggctcagg catgggccgg gccggttcag gcaatccaaa
     1201 gtggccaaga atgaatatag aagcgacgag aagaacgagg atcgacagga ccacgtcgcg
     1261 atgttcgccg agtcgctaat tccggcaaat gaaattcccg gcacgagttg acgcagagca
     1321 cggggatgca gtcatgagtc atggcggaac tcacgcgcag actgcaaatc ctgctcgatc
     1381 cggatcaaat ggagtcattg caggcaatcg cgcaaaagcg gcgtaagtct gtggcggaac
     1441 tcatccgcga agcggtcgac attcagtaca gacctcgcac tgaagtaaga cgcattcagg
     1501 ccatagaaag tcttagatcg ttctcttcga tccacatttc cacggacgat ttgaagtaat
     1561 ctgaaaattt tgaagtagtc tgaaaatgga aaagtggttc ctcagttttg atgccatcct
     1621 ggttctcgcc tccgatctac ccgcgaagtc cgcgcttctc gatcaactga gcgaatttct
     1681 aaaatcaggc attctggtta catcctcgca ctcagtgcaa agatgcgccg agtttttcga
     1741 acacagagga gagaaatcgt ttcgatcctt ctattcccgt ctgaggcccg ctctggatca
     1801 tgttctggaa gtgacggaaa gagatcagtc ttacgctctg gatctggccg aatcttctaa
     1861 catatctctg gcgctggctc tcgaagtggc cgtggcgatt cactcaggtg ccagtggctt
     1921 cgtatcatcg aattcaaaaa ttgactcgct ttcggaactt ccgcgcatcg ctgttgccat
     1981 atgagccagg aacaggtagt agaactgaca gaagaagagt cttccacgca ggagctcgcg
     2041 cgtcgcattt caaatcagga cctggctccc atggcagatg agctcgatcg ccatgagcag
     2101 ttcgcgcacc cggtgtttca gaaactcgga gaagctggac tactcggaat tgtcattccg
     2161 gaggagtttg gaggggcaga tggatcgttt ttgtctgcag ttcttattat ggaagaactt
     2221 gcgcgaaatt gcggcgggac tgctctgagt tttggagcgc acgcattcat tggcgcttac
     2281 aaccttttca aattcgcaag tgattcgcag cgaaagaaat atctgcctga tttgtgttct
     2341 ggtaagaagt acgccgcctt tgctctcact gagccggaga acggatcgga tgcgatgggt
     2401 cttcgcacca gggcagagaa gcgtggagca tcttacattc tcaatggcgc caaaactttc
     2461 atcacaaacg gaagcgtcgc agacacattt gtagtcttcg cgcggacggg agatgcaggg
     2521 gccaaaggaa tttccgcatt cattgtggag agatcttttc cgggatttgc atcgggtaaa
     2581 cccatggaaa agatgggaat gagagcgagt ccaactacgg aattgtattt cgaaaactgc
     2641 gaagtgcccg cggagaattt gctcggtgcc gaggggcagg gcggttactt tgccctggaa
     2701 ggtctggaca tggagcgcac tcttttttca ggcctgccga ttggactgat ccagggagca
     2761 tttgatgttg cgctcaagta cagcacggaa agatctcagt tcggttcaaa gatcgctggc
     2821 cttcagctga ttcaggccat ggtggcagac attggaatga atttgttcgt gtccaggctt
     2881 cttgcctaca cagcagccag aaagctccag gaaaaaaaga gagtcactct caacgcaagt
     2941 ttctccaaac tcttcgcatc ggaagcaagt gtgaaaagca ctctcgactg catccagatg
     3001 ctgggcggtt acggctacat tcgtgaattc aaagtagaaa gactgatgag agatgcaaag
     3061 cttgtggaga taggcggagg cacttctcag atccagcgtc tcatcatcgc tcgcgaaatc
     3121 tacaaatcgc acgatttgag cccttagaac attttttgtt tttacttgat ctctggcgca
     3181 gtcgggtcgt ctaatcgatt atgccagatc ttaacaccga ccaatcccgc ggacaggtgc
     3241 agaaccttcc atccgcatcg agcgggccac gcgccattga accggttgtt gttccagaaa
     3301 cagaacttcc gcctgccaca ggtgcgcaaa gaatcttctg gatcatcatt gcgattttgt
     3361 gcgcagggtg gatcttcatt ccaaatttct tcatcattcc gattcctttt ttcatagatg
     3421 aaggaatcgc ggcgatcatt ctgatgaccg cactctcgaa gcttggaatc cgcatcccca
     3481 ttctccactg gttcttcgag aagagaatgg gaaagagcat tgctctgaag actaagcgct
     3541 gatcaggttt cccgacccca gtgtgtcccc tggcgatctg ttggaaatgg tctttccgca
     3601 acgccagtgt acacctgtcc gggtctgtat gctccacgca ctccttcgtc gtgctgttcc
     3661 atccagtgcg ccagccagcc tgaaagtttt ccaatcgtgc gaatcacgtt gttcatagtc
     3721 atgggcaggc cgatcattcg aaagatgagc gcggagtagt aatccaggtt cggatacaga
     3781 cccttctctt tgaaataggg atggcccagg gtgaattctt ctacttccag agctttttcg
     3841 aagagaggat ctacggccgc aatttcgttt gtcttcctga attcgtgaaa gagtttcctg
     3901 gcaatgatcg cacgaggatc ctgagtcctg taactcgaat gaccgaggcc gttggaacgg
     3961 atgatttcct gcccttttat aaacttttca aagtattctt ccgggctctg gcgacgtttt
     4021 gtcatctggt cgagcatggg gataggcgga atgtttgctc cgaattccct ggatccccac
     4081 atcgcacaca tcgcggcgtt gatgcaggca aacagatttg cctgtgagct tgccacgagg
     4141 cgcatcgtgg ttgtagcagt gttctgttca tgatcgctat agagaatgag caactgattg
     4201 agcactcgat cgtgtgattt aggaacttga tatgtttctg ccggaacagc aaagagcatg
     4261 ttcaagaagt ttgtgcaata tggcatttcg tcgcgcgggt atactactgg ctctcctaca
     4321 gatttcttgt aagaccaggc agccagtgtt cgcactttgg caagaagcct cgcgcgaata
     4381 tctactccct ttttcaaatt ctcttcatac gtagacggat agtatgcgga cagagcagtt
     4441 accatggtgg ccaggatggc gattggatgg gcgtctcctg gaaaagcatc gaagaagtag
     4501 cgcatggatt cgtgaatcat ggaatgcttg cacaactcgt ttgaatagtt tgcgtactgt
     4561 tcttgatttg ggagttctcc ttcattgagc agaaaggaaa cttctacgaa gctggagtgc
     4621 tctaccaggt cctcaatgcg atagccacga tagagtagcc ttccgctcac tggatcaatg
     4681 tatgtgatgg cgctcttcgt gatgttgacg ttgttcaagc gcggatcaaa aatgcgaagc
     4741 cccgtctttt caaagaggct gtctgcgtga attgaacgcg agccatctgt accctgcacg
     4801 acgggcaggt ggtattcttg attgtctaga atgagttttg cgtcttccat agcctggcat
     4861 gattcggcgc caggcgggca tggttgcaag cactatctct cgggttattt ccgcttcagt
     4921 ttgtgcttga tgtaggctgc aaattcaccc agagacatgt tcccggatag ctttggcgcg
     4981 ggcatagaat ctggcaccgg gactgcctct gcagtctgca tggccggctc agctgcaatg
     5041 gcagatgcaa tcggcacaat tcgaatttca cccgtaacat tcttctgcac ttccactctc
     5101 tccgatttca gtttgatatc aatgcgcaag aacttgcgat tgatgatcga gatggccatg
     5161 gagccatcca gaattcgaat gatttcgcga acgatcgccc atttggccca gagagtgcct
     5221 cgttccggat tcaaactcgt gatgagttcg tgaagcttgc gcgtctggcg aaagtcgtta
     5281 tggaaaagaa gaaagcgaaa gtgaatgttt tctgaaaggt cagatgtgat gatcaggtca
     5341 atgtgcttga actcgcgaaa ccccgggaag tcgatcaggc gaatgaccat cgagttgacc
     5401 agttccaggg atgttttgat ttcaatgtca ggatccacgt tgatgtagga agtgatcgct
     5461 ccctgcggaa tcaccgctgt gataaagtcc ttgaaattta cattttcaaa agattccggc
     5521 tcctggatca cttcgagtct cgatagttcc aggatgtcgt ccatctccgc ctgcagcacg
     5581 cccaggtctg tgtcgatttt atccagcagt tccttttttt ctttctctct gaggtccgcg
     5641 tttcgcgcgg caagagtcag gttgacgatt gcctgagcgg gcccgcgaag aatcccgtca
     5701 atgtatctga acacgcggtc tcgcagcgcg tttacagaat ccagacgagt acgccgtctc
     5761 tccacgtcca ggtgaagccg aatcaaacgg aaaatcagga tgaaagaaag cgcgaaaatg
     5821 aaaatgaata cgctcgaatc aaagactcct ccgccccact tgatgaatcc acgttcgctg
     5881 aggaagaata gaagtgtggc cacgagcatg atgatcatgc caccggcgag aatcattgca
     5941 tctgagtccc ggcgaagtgc tcgctggcat gcatgatacg cataccatgc aaaaaaggga
     6001 aggttcagcg ggaaccagat ggtcatcact gcgtcgattc ttacaggcga gccaaggaat
     6061 ggcaggatca gagccacaac tccgttgaaa gccggataga ggtaaaccag gcgattgagc
     6121 ttgagtttaa aaaagctaac aaagaaaaag aagaaggaag tcgcgcatgc aatgtagagc
     6181 atttgctctg ctgccttgaa tgcataaaac cagtctgcga tctggaaacg cagttcgttt
     6241 ctcatcagtt gctggagagc aaaggaaata gtaaataaac tatagtaaaa atagtctcgc
     6301 atcccgccca cgcggaggaa tagcactatg aagaagatac ctgtggcaat gtagattccg
     6361 gaaagaacca ggcttttgag ggcagttttc cagacagttg cttccgctcg atcaatcgtg
     6421 tcgattcgca ctggcccgga agtgattccc atgaagtctt tgaaagaacc ttccaggcga
     6481 attgcaattg tgttttcgcc ctgacgaaga atagaagcgg ggagtgggta cactcgcggt
     6541 ctgccatacc cgtatttctc ggatgatttt tctccaatga gttccccatt ca
//
LOCUS       DAORFF010000168         6582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6042_length_6582_cov_3.567642, whole genome shotgun sequence.
ACCESSION   DAORFF010000168 DAORFF010000000
VERSION     DAORFF010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6042_length_6582_cov_3.567642"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..898)
                     /locus_tag="PKK76_08325"
     CDS             complement(23..898)
                     /locus_tag="PKK76_08325"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL68518.1"
                     /translation="MDEDHILTLLAEHALIREDQAARVKEIHASTGRPTGSILLSERF
                     LDEKQLYAFICQDLGMNPASVYQDKFYEISGLTIPKFEVSGDFYGSFMLDEGRIALTL
                     SDVSGKGIEAGLLAILLANILRTGVRMKNIIPSMILRKVNHASTRFFGEEQFATFIVL
                     ILDMHSGTVEFCCAGSPPILVYRRRENYVEQLEVKGIPVGIYDDFLFNGSRTSIEKGD
                     VILMYTDGAYENQNFRNEYFGLRRVKDSLRKNSPRTARKIIHAIKREIKLFSMFRGLN
                     DDTTFVSIKRSDRGS"
     gene            1002..2354
                     /locus_tag="PKK76_08330"
     CDS             1002..2354
                     /locus_tag="PKK76_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNL68519.1"
                     /translation="MAILDEKDLTRLDKYRHILDRLDPLRPMGRVVEVRGLSLISDGP
                     PDASIGDICRVAVKSGDYIRAEVVGFQKDRLVLMPLGGTAGISPSADVLASGRRMQIA
                     VSEKMLGRVFDALGHPLDMKEGITSSEERFHEGEPPSPHVRVPIRTPLETGVRAIDGL
                     LTIGRGQRIALMAGTGVGKSTLLGMICRYTRADINIVCLVGERGREVREFIENDLGEG
                     LEKSVVFVAESHRTAMEKVYCAGFATSVAEYFRDKGKHVNLIVDSLTRYCWALRDIGT
                     ASGEMLGPGGFPPNVWAKLSKLVERAGVTHEGSITGFYSVLVEGDDLNEPVTDHARGM
                     LDGHIVLSRRLAERGHYPAIEVTQSISRVMDKVISPEHLQDASRMKTLLSAYRENEEL
                     LNLGAYARGSNVDLDEYLDKASAIQGYLRQRVEEGVHIEDSVARLSDIIRPKIVEEAY
                     "
     gene            2386..2946
                     /locus_tag="PKK76_08335"
     CDS             2386..2946
                     /locus_tag="PKK76_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68520.1"
                     /translation="MLKKRQIEQDLALKKLSVVLARYNKQEAARREAFELLKEETNLF
                     EKKYREEFELDLFQTYDRYLERLEANAADAARKMEEMQPELAAERELVMIARRNTRII
                     EKLKEHYKKDYEKENQLLEKEELFELNSRIAGMEEQHGESGIIEERRKPPEEPDSEDE
                     FPEDTAADLVSEYFRRLGLQDPRKKR"
     gene            3136..3807
                     /locus_tag="PKK76_08340"
     CDS             3136..3807
                     /locus_tag="PKK76_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68521.1"
                     /translation="MSRLSEKIKIVYLTLVILFSMGVFAYLLDTWHIIKLKDHIPFLK
                     HEPPVVGEETDDASLRKEEQLQKEEQRLQELELKLKEAEAKLGEQTGNVQKQQEEIEL
                     MRKGLAEERKKLEEQKQAAFQRQKTVKEMSGRLLNMPPDDAVAICANWSNADVVDVFL
                     QMERDAEAEGRQSIVPFLLTKLPRERAGVITTLMMDERARMLPDTTDGPAPESAPSPA
                     PTPAP"
     gene            complement(3815..5062)
                     /locus_tag="PKK76_08345"
     CDS             complement(3815..5062)
                     /locus_tag="PKK76_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL68522.1"
                     /translation="MTRIGPGAYSTVILHLDPDEAYRSEIGTLLHANGLKSKGARSLG
                     EALELVTRHAVKVVVTELDHPDAHAEQILESLHRLDPSLAIIVLTSRPERRQGPTRRL
                     GRVFEFVEKSQPPHILLSHIQRGRDYYLQRHHDNVIAGESEGKLRSKLEWLIWKQQAK
                     LKVVDNYATLVNNVKHSVAQGAGLDVMCTLVDLLMATATEVDGKMMIEAEVLPALNEA
                     AASARRWLENLDKMLSMTHLLHGKKDKTTAFHTSVRNAIASVHHLQIQKNQTIALGGL
                     LECSVMMQQEGFELCVRELLTNAFKYSPESSRIDITAHRAGDSASLTVLSDVMESKGN
                     IDGIPAGSEEVLFEPLVRLNNSYDERFRDEELAMGFGLAIVQSLVSSAGGRAYIREIR
                     DFSVDFVARRRVLSEIVVPILKN"
     gene            complement(5087..5452)
                     /locus_tag="PKK76_08350"
     CDS             complement(5087..5452)
                     /locus_tag="PKK76_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68523.1"
                     /translation="MNQSHAVPERVHFLCAACGKIAGTMWLEEGQECNLVRESFTGRL
                     TLPIDPQNRTPIGHWLRERNAQALHDFDFEIASFYCPSCDLNYCREHWSARDVFDDDG
                     WHDSIRGTCPAGHERMLED"
     gene            5772..>6582
                     /locus_tag="PKK76_08355"
     CDS             5772..>6582
                     /locus_tag="PKK76_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNL68524.1"
                     /translation="MNLRWFREHGISVVEFLRASHVLSGLDRPALKALARSFVPITLQ
                     PGESVMKQGEPGDSLFLVASGRLRATRVRADGSETTLGEICTGEIVGEAAVLTDEPRY
                     ANVSVVEQADLLRLSRTDFNSLLATHPAEIRKLSHIIADRQEQGHTERFRPVSRNLIE
                     FLKNVPLFAFLPGPLLKEIEPHLTWLHLPAGRVLMRQGEEADGLYVVVGGRLRFESVD
                     ERGVKRSGDFGRGEIIGELALLTGDSRSATVRAVRDSELVKLSDVSVQRMLH"
BASE COUNT         1616 a         1630 c         1733 g         1603 t
ORIGIN      
        1 gcacaaacgc ctgcgagttc tttcaactgc ctctgtccga tcgtttgatg gatacaaaag
       61 tagtatcatc gttcaggccg cgaaacatgg agaagagttt gatttcgcgt ttgatcgcgt
      121 ggatgatctt gcgcgcagtg cgaggcgaat tctttctgag gctgtctttg actcttctga
      181 ggccaaagta ttcatttctg aaattctggt tctcgtaggc gccgtccgta tacataagaa
      241 tgacgtcccc tttttcaatg gatgttctac ttccgttgaa aaggaaatcg tcgtaaatgc
      301 caacaggaat gcccttcact tccagttgct ccacatagtt ttcgcgtctc cggtatacaa
      361 gaatcggagg gctgcctgcg cagcagaatt caactgtacc gctgtgcatg tccagtatga
      421 gcacaatgaa tgtagcaaac tgttcttctc cgaagaagcg agtgcttgca tggtttacct
      481 ttcgcagaat catcgaagga atgatattct tcattctcac gcctgttcta agaatgttgg
      541 ccagcagaat ggcaagtaag cctgcctcga tgccttttcc agatacgtcg cttaacgtta
      601 gcgcaatgcg accctcgtcc agcatgaagg atccgtagaa gtctccgctc acttcaaact
      661 tcgggatggt caaaccgctg atttcgtaga acttgtcctg ataaacactg gccgggttca
      721 tccccagatc ctggcagatg aaagcataga gctgcttttc gtccaggaaa cgctcggaca
      781 gcagaatgct gcctgttggc cgccctgtcg aggcatggat ttccttcacg cgagccgcct
      841 gatcctcccg gatcagtgca tgttcggcca ggagggttaa aatatgatct tcgtccatcc
      901 ctctatcgcc acttgagaca taaattttcc atcggatact aaaaaacgat tgtactgaaa
      961 atcggcatcc ggcatccaag aatacaccgg gctacgtcac tatggccatt ttagatgaaa
     1021 aggatttaac gagactagac aagtaccgcc atatcctgga ccggctcgac ccactgcgcc
     1081 cgatgggccg ggtagtggaa gttcgcggac tttcgctcat ttcagatggt ccgccagacg
     1141 caagcatagg tgatatatgt cgcgtcgccg tgaaatctgg cgactacatt cgcgcagaag
     1201 tagttggatt ccagaaggat cgcctggtgc tcatgcctct cggaggaaca gctgggattt
     1261 caccttccgc agacgtgctg gcgtccggca ggcgaatgca gatcgctgtg agtgaaaaaa
     1321 tgctcggtcg cgtctttgac gccctgggtc atcctctcga catgaaggaa gggattactt
     1381 cttcggagga gcgttttcac gaaggggagc caccttctcc tcacgttcgt gttccaattc
     1441 gcactccgct ggaaacaggc gttcgagcga tcgacggact tctgacgatt ggacgcggcc
     1501 agcgtatcgc gctcatggct ggcacaggcg ttggtaaatc gacactgctt ggaatgatct
     1561 gcagatacac gcgcgcggac atcaacatcg tatgcctggt aggcgaacgc ggtcgtgagg
     1621 tgagagaatt cattgagaac gatctaggcg aaggtctcga gaagagcgtc gtgtttgttg
     1681 cggaaagtca taggaccgca atggaaaaag tttattgcgc cggctttgca accagtgtgg
     1741 cagagtattt tcgggacaaa ggaaagcacg tgaatctgat cgtggattca ctgacccgat
     1801 actgctgggc tcttcgagac attggaactg cgagcggaga gatgcttggt cccggaggat
     1861 ttccgcctaa cgtctgggca aagctcagta agctagtaga acgtgcgggt gtgacgcacg
     1921 aagggagcat tacaggtttt tattcggtgc ttgtggaagg tgacgacttg aacgagcccg
     1981 tgaccgacca tgctcgaggt atgttggatg gacacattgt gctctctcgc agactcgcgg
     2041 aacgcggaca ttatccagcc attgaagtca ctcagtctat ttctcgtgtg atggacaaag
     2101 ttatatcacc tgaacatctg caggatgcca gcagaatgaa aactctcctc tctgcgtaca
     2161 gagaaaacga agaattgctc aacctggggg catacgcccg tggttccaat gtggatctcg
     2221 acgaatacct ggacaaagca tccgctattc agggatatct tcgccagaga gtagaagagg
     2281 gtgttcatat tgaagattct gtcgcgaggc tgagcgatat cattcgtcca aaaatagtgg
     2341 aggaagcgta ttagaaaaaa gaagttccgt tttccgctcg aagcgttgct taagaagcgc
     2401 cagatcgaac aggatctggc cctcaagaaa ttgtctgtcg ttctcgctcg ctacaataaa
     2461 caggaggcag cgaggagaga ggcctttgag ctcctcaaag aggagaccaa tctcttcgaa
     2521 aagaagtatc gcgaagagtt tgaactcgat ctgttccaga cgtacgatcg ctatctggaa
     2581 agactcgaag caaatgcagc agacgctgcg cgaaaaatgg aagaaatgca gccggaactg
     2641 gctgcggaac gagagcttgt gatgattgcg cgaagaaaca ctcggatcat tgaaaagcta
     2701 aaagaacatt ataaaaaaga ttatgaaaaa gagaatcagc ttctggaaaa ggaagagctt
     2761 ttcgagctga attctcgaat cgcgggaatg gaggaacaac atggagaaag tggaattatt
     2821 gaagaaaggc gcaagcctcc tgaagaacct gactcagagg atgagttccc cgaagacaca
     2881 gccgcagacc tcgtttcaga gtactttaga agactcggtc tccaggaccc gagaaagaag
     2941 cgataacacg gaaggagttt tgtctcgctg gttcactaag gatccaattc gtgagcgtca
     3001 aatggaaatc cagcgcacaa ggaatgacct ggaagaattc aacgtgacga ttgcagattt
     3061 gaaccgaaaa attgaaagaa tggagaagat caccgcgaaa tggaaagagc gcggcgagtc
     3121 ttttgacagc agggtatgag ccgtctttct gaaaaaatca agatcgtcta tttgaccctg
     3181 gtgattctat tctccatggg cgtattcgcg tatcttctgg atacgtggca catcattaag
     3241 ttgaaagatc atattccttt tctcaagcac gaaccgccgg ttgtgggtga agaaacggac
     3301 gatgcgagtc ttcgcaaaga agagcaactg cagaaagaag agcagcgctt gcaggagctc
     3361 gagctcaaac tgaaggaagc ggaagcaaag ctgggtgagc aaacggggaa cgttcaaaag
     3421 cagcaggaag aaatcgaact tatgcgaaag ggtctcgcag aagagcgcaa gaaactggaa
     3481 gaacaaaaac aggcggcgtt ccagagacaa aagacagtca aggaaatgtc aggcagactc
     3541 ttgaacatgc ctccagatga tgcggttgct atttgcgcga actggagcaa cgcagatgta
     3601 gtggatgttt ttctgcaaat ggagcgcgac gcggaagctg aaggtcgtca gtccatcgtt
     3661 cctttccttc tcacgaagct tccaagagaa agggcaggcg taatcacgac cttgatgatg
     3721 gatgagcgtg ccagaatgct tcctgataca acggatggtc cggcaccgga aagcgcgcct
     3781 tcgcctgcgc ccactcctgc tccatagagc cggtttaatt tttcaggatt gggactacta
     3841 tttcggaaag aacgcgtctt cgcgcaacaa agtctacgct gaagtcgcga atctctctta
     3901 tataggcgcg tcctccggca gaagaaacca ggctctgaac aattgccagc ccaaagccca
     3961 tcgccagctc ttcgtcgcgg aatcgctcat cgtaggaatt attgagtctg acaagtggtt
     4021 caaaaaggac ttcctcggat ccggccggga taccgtctat gttgcctttg gattccatta
     4081 catcactgag cactgtgagg gaagcagaat cgcctgctct gtgagccgtg atgtcaatcc
     4141 tcgaactttc cggggaatac ttgaatgcat tggtcaagag ctcgcgtacg cacagttcga
     4201 aaccttcctg ttgcatcatc acactacatt caagaagacc tcccagtgcg attgtttggt
     4261 tcttttgtat ttgcaggtga tgaaccgatg caattgcatt tcgaactgat gtatgaaatg
     4321 cagtggtctt atcttttttt ccatgcaaca gatgtgtcat actcagcatc ttgtccaggt
     4381 tttcaagcca ccgccgtgca gacgctgccg cttcgttcaa cgcgggaagc acttctgcct
     4441 cgatcatcat ttttccgtct acctcagtgg ctgttgccat cagcaaatcc actagagtgc
     4501 acattacatc aagccccgcg ccctgtgcca ccgagtgttt tacgttgttc accagagtag
     4561 catagttgtc cacaaccttg agtttggcct gctgtttcca tatcagccat tcaagtttgc
     4621 ttcgcaattt tccttcggac tcgcctgcga tcacattgtc gtggtgccgt tgcagatagt
     4681 agtctcttcc tctctgaatg tgcgaaagca ggatatgagg aggttgggat ttctctacaa
     4741 actcgaaaac ccgtccaagt cttcgggttg gtccctggcg ccgttctggt ctactcgtga
     4801 ggacaatgat ggcaagggaa ggatcgaggc gatgaagcga ttctagaatt tgttctgcgt
     4861 gcgcatccgg gtgatcgagt tcagtgacca ctactttaac cgcgtggcga gtgaccagct
     4921 cgagcgcctc tcccagcgat cgcgctccct tgctcttcag cccatttgca tgcaacagcg
     4981 ttccgatttc ggatcgatag gcttcatccg ggtccaggtg aaggatcact gtgctgtatg
     5041 cgccggggcc aatgcgcgtc aagacaaacc ctggacaatt tagatttcaa tcttccaaca
     5101 ttctctcatg tcctgccgga caggttccgc ggatcgaatc gtgccagccg tcatcatcaa
     5161 aaacatctcg tgcgctccag tgttcgcggc agtagttcaa gtcgcacgac ggacagtaga
     5221 aagatgcgat ttcaaaatca aaatcgtgca gtgcttgtgc gtttctttct ctcaaccagt
     5281 ggcctattgg tgtgcgattt tgcggatcta tgggaagagt caggcgtcca gtaaacgatt
     5341 cacgcacgag gttgcactcc tggccctcct ccagccacat agttccagca attttgccgc
     5401 aggccgcgca cagaaagtga acccgttcag ggactgcgtg tgattgattc aaatgtgtgg
     5461 cctcccgaac gtcactcttc cctctttgat gactggcatt acccgtgtca gacacatttc
     5521 gtcaattgcc gcatgcgaag cgcactattc ttttccatcc accagaccac cagagggccg
     5581 cacaatgccc atccggaacc ttcacgagcg aacgatagac ggctttgtga tcccttgcat
     5641 tgcgaaaaag cgagagctga atgcgatcag acttctttca gcttcggaag tttttctcaa
     5701 atcttttccc gttctcctcg ctcccggtgg atgtcgcttg acatgcttgt tcagtggtct
     5761 caaggcgtac tatgaatctt cgctggtttc gtgagcatgg aatttctgtc gtcgagttcc
     5821 tcagagcatc gcatgttctt tctggtcttg accgcccggc tttgaaggca cttgcccgct
     5881 cttttgttcc aatcacactc caaccaggcg aatctgtcat gaagcagggg gagccggggg
     5941 attctctatt tctcgtcgcc tccggtcggt tgcgcgcaac gcgagtacgt gcagacggga
     6001 gtgagaccac cctgggcgag atttgcacag gcgaaattgt aggggaagct gctgtcctca
     6061 cggatgaacc tcgatacgcg aatgtctccg tcgtggaaca ggcagatctg ctgcgcctgt
     6121 ctcgaaccga tttcaacagc cttcttgcaa ctcatcccgc agagatccgc aaactctcgc
     6181 acatcatcgc cgaccgtcag gagcaaggcc atacagaaag gtttcgtccg gtttcgcgca
     6241 atctgattga atttcttaag aatgttccgc tctttgcgtt tctgcctggt cccttgctga
     6301 aggagatcga gcctcatctg acgtggctgc atcttcctgc tggccgtgtg ttgatgcgtc
     6361 agggggagga agcggacgga ctttatgttg tggtgggtgg ccgcctgcgc tttgagtccg
     6421 tggatgagcg cggagtcaaa cgctcaggcg actttggccg cggcgaaatc ataggtgagc
     6481 ttgcacttct gactggagat agtcgttctg caacagtgcg cgcggtgcgc gattctgaac
     6541 tggtaaagct atccgatgtt tctgtccagc gaatgcttca tg
//
LOCUS       DAORFF010000169         6571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6052_length_6571_cov_3.214702, whole genome shotgun sequence.
ACCESSION   DAORFF010000169 DAORFF010000000
VERSION     DAORFF010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6052_length_6571_cov_3.214702"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKK76_08360"
     CDS             complement(<1..500)
                     /locus_tag="PKK76_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68525.1"
                     /translation="MKPGTFWRLTWRLELFTHLIPVPSAIYFSAVAGGFPENEFILAI
                     NVGAIGGGLVVVLGVIWRYFRLQSIERRVAALDAGSLDEARARRLKIEILRYPRDEAI
                     VIILRWITGVPIVHILYIILNGLRISTHITIPFVFLLTTPISAVVYLFISEREVRPLL
                     LRESLA"
     gene            complement(497..1543)
                     /locus_tag="PKK76_08365"
     CDS             complement(497..1543)
                     /locus_tag="PKK76_08365"
                     /inference="COORDINATES: protein motif:HMM:NF015252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HNL68526.1"
                     /translation="MKNKKLLFLGGGAAIIVVAIAAFLIPGRDPVRKKFEELESKTSK
                     YTPSESVLESIKMKDAGDGHLTYDGELTFDQTVALLRKKYGAKINHPLVQMRMLQELL
                     RYLQKKYPNNWVEYLHELLGAAFPELAQKLFTMSKNLYLYDKFLNDTRDKATKMGDGD
                     RDKFLWSKRYELFGSDADEIWAGEKKYNDVKNALNDIANNRTGTIKEKLSQFKDTLNK
                     TYGDTLPSVMESRRQNLLDGFVGAVQTDLQSMTPADRREAMHDIRSSMGMDDAAITRW
                     DALDAERDQRWSNGQIYTNERKQIISQYTGTDREQKLDDLRHKLFGDEADTIKSEEAS
                     GYFRFDGKQQYGIN"
     gene            complement(1661..2785)
                     /locus_tag="PKK76_08370"
     CDS             complement(1661..2785)
                     /locus_tag="PKK76_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNL68527.1"
                     /translation="MKKAIVFGLALSLTGIMTSANATVRKTANPVVFAHGLAGWDNIL
                     GAFYFGDNNGNFVGDPCDEFLEVLCNGGLNSSQKAYAASVQPFQSSEVRGTQLADKIQ
                     SYMTSVGASYVNIIGHSQGGIDARKAAVVLKQRKGYAVVKTLVSVSSPHRGSPVAKYI
                     LDLKPGVVSVVDALAQLFGNVIYGSGNDGYAAAKQLVYNDYSSTDGVTTGMKAFNTAY
                     ASYNTAVSNRLASVITAQQGLDVNPALYILSKGFYDIDGDGATCAVASDDCDNDGALG
                     KGNGSKTDTDDDGLVGVNSQQMGYRLKYSECFLCDDYVTQDTTLGYLTDLNNPTSAQQ
                     SSHADVLNQDHLDVTGVGPDTMDENEFYAAIIDYMSANGG"
     gene            3058..5859
                     /locus_tag="PKK76_08375"
     CDS             3058..5859
                     /locus_tag="PKK76_08375"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNL68528.1"
                     /translation="MRQSTMAGLILLAGLFVLPRAASAQIKPSVEILEDPTASLTSQT
                     ANISKNWKHFGSATPNLGYSRSTHWVRFDLEAPAAGEYIIEVAYPLIDLLECTILRAN
                     SESVETQGRLRSQADRLPLFHLSLAEKEKVRVIMRVQSDDSITFPVRIYTPQEHEHEQ
                     NTRQFLFGLYYGVIAVMILYNLFLYYFVRDQAYLYYVLTLVLLHGLFQLALNGFARMF
                     LGSELLWWKKGAIVFFHSAGVLAAILFCRVFLLIPRVAKKWDYIARAIIVWGALNMLI
                     SFFDLYRLVILSTVGLGILGIVFALASGIIAMRRGYRPARFYLLAWSALLFGGFIYVL
                     KIFGIIPSYWLTEYGVQIGSAVEATLLSIALGDRITTGRQDQVKARTEMLVHKRIALL
                     AQTELLNHLSQLDSLKSQMHSIEYEGNSLARLLENILETLRKILSFDKGFIVIADRLQ
                     KTYLRSVGSMQPDFYEKIPDDHYLHMLIKLPDALFDQVNRIVHLDRPVFENIPENSSQ
                     VTQVMKALEDTVAALRETGFSLCLPLAYEREIFGYVMLSARKSGEQYTNSELHLIDTF
                     RPSIAMAVRNAVLYEEVVLLRGRAEQKAAKLSDVIIDMKDTTRFDLKEKALVYISHPM
                     AEIYQTVRKYADKPQPVLISGETGTGKELIAQTIHEVGGLSREPFIPVNCAAIPASLW
                     ESEIFGHEKGAFTDAKSERAGKIEQAAGGTLFFDEIGEMPLELQAKLLRLIQERKYER
                     VGGKKSLEARCRFLFATNRDLQVMQKAGTFREDLYYRISVFQIVLPPLRERREDIPVL
                     VNFFIRKYAAEMQVPVSHIEKAAMESLTYYDWPGNIRELENCVIQTLVNSTSDTIRVE
                     DIPGHLSSRGQVVSKEALRTWDPDMDFRELDAMVSDYTRQVMIAAIQRCAGNKQEAAK
                     LLGVKRATFYYRLKELGIQ"
     gene            complement(5861..>6571)
                     /locus_tag="PKK76_08380"
     CDS             complement(5861..>6571)
                     /locus_tag="PKK76_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNL68529.1"
                     /translation="NCPGAQVGSTYSMTHAEAWSFAPRHQAARDRFDAVINRLFLDPA
                     CGRGYPWDTVPSLKKIQKHMKSGDDAKLKCDFDFIGIQNYTRDVVRRSILMPYVWARP
                     VSAKKRRVPETEMGWEVYPQGIYELLRKFGSYPEIKKIYVTENGAAFPDQVQDGRVHD
                     EKRIAFLRAYIGEVIRAKRDGSPVHGYFVWSFLDNFEWAFGYRPRFGLVHVDYETQVR
                     IPKDSALFYRDLIAGKDV"
BASE COUNT         1652 a         1692 c         1601 g         1626 t
ORIGIN      
        1 tgagccaggc tttcgcgcaa aagcagaggt cgaacttcgc gctctgaaat gaacagataa
       61 accaccgcgg atatgggcgt ggtaagaaga aatacgaaag ggatcgtaat gtgagtggat
      121 atgcgaagac cgtttagtat gatatacagg atgtgaacaa tgggtacgcc tgtaatccag
      181 cgcaggatga tcacgattgc ttcatctcgc ggataacgaa gtatttctat cttcaggcgg
      241 cgggctcgcg cttcatcgag agatccagcg tcgagggctg cgactctgcg ctcaatggac
      301 tgcaggcgaa agtaacgcca gatgacgccc agcacaacca caagaccgcc accaatggcg
      361 cccacgttga tggccagaat gaattcgttt tccggaaatc ctcccgcaac tgcggagaaa
      421 tagatcgcac tgggaacagg aatcagatga gtgaaaagtt ccaggcgcca tgtaaggcgc
      481 cagaatgtgc ccggcttcaa ttgatgccgt actgctgctt tccatcgaag cgaaagtagc
      541 cagatgcctc ttcactcttg atcgtgtcag cttcgtctcc gaatagctta tgacgaagat
      601 cgtctaactt ctgttcacgg tctgttccag tgtattgcga aatgatctgc ttacgttcat
      661 tggtataaat ctgcccgttg gaccatctct gatcgcgctc cgcgtcgagc gcgtcccatc
      721 gcgtgatagc agcgtcgtcc attcccatgg aactacggat gtcgtgcatt gcttctctgc
      781 gatccgcagg tgtcatagac tgcaaatctg tttgaaccgc tccaacaaaa ccatcgagca
      841 gattctggcg tctggattcc atgacactgg gaagagtatc tccatatgtc ttgttcagtg
      901 tgtccttgaa ctggctgagc ttctccttga ttgtgcccgt gcgattgttt gcaatgtcgt
      961 tgagagcatt ttttacatca ttgtattttt tttcgccggc ccagatctca tccgcatccg
     1021 atccgaacag ctcataccgc ttgctccaga ggaacttgtc tctgtccccg tcccccatct
     1081 ttgttgcttt gtctcgtgta tcgttcagga atttgtcata caaatacagg ttcttggaca
     1141 tagtaaaaag cttctgagcc agttcaggaa aggccgcacc cagtaactcg tgcaggtatt
     1201 ccacccagtt gtttggatac ttcttctgca aatagcgaag aagctcctgc agcatcctca
     1261 tttgcacgag ggggtgattg atcttcgcgc cgtacttctt ccggagaaga gcaaccgtct
     1321 ggtcaaaagt aagctccccg tcataagtca aatgaccgtc gcccgcatcc ttcattttga
     1381 tgctttcgag cacagattca gatggagtgt atttgctggt cttactctcg agctcttcaa
     1441 atttcttgcg aacaggatcg cgaccgggga tcaaaaatgc cgcaatggca acaacgataa
     1501 tggctgcgcc gccaccaagg aatagtagtt tcttattttt cataatagtg atgcattcag
     1561 catgtttgaa tgtctgtggt atatcgcgcg cgaagggata acgcggtaaa taaaaaaccg
     1621 cccctgcgaa gaggcggttt cgaaatttag caaacttttc ttagccaccg ttcgcggaca
     1681 tgtagtcaat gatcgcggcg taaaattcgt tttcgtccat cgtgtcaggt ccaacgccag
     1741 ttacatccag gtgatcctgg ttgagcacgt cggcgtgtga gctctgctgc gcggatgttg
     1801 gattgttcag atccgtcaga tatccaagag tcgtgtcctg tgttacgtag tcgtcgcaca
     1861 ggaaacattc agaatacttg aggcgatagc ccatttgctg cgagttcaca cccacgagac
     1921 cgtcgtcatc cgtatctgtt ttggatccgt ttccttttcc aagtgcgccg tcgttatcac
     1981 agtcgtcaga agcaacggca caggtggctc catcgccgtc tatgtcatag aaacctttgc
     2041 tcaaaatgta gagcgctgga ttgacatcga gaccttgctg tgcggtgatc acggaagcca
     2101 gacgattgga caccgcagta ttgtagcttg cataagcggt gttgaacgct ttcataccgg
     2161 tggttactcc atccgtggag ctgtagtcat tgtacaccag ctgcttggct gccgcgtatc
     2221 cgtcgttgcc ggatccatag atcacgtttc cgaaaagctg tgccagagca tccactacgc
     2281 tcaccacacc cggcttcaga tcgaggatgt atttggcaac aggtgatcct ctgtgaggcg
     2341 aagatacaga tactagagtc ttcacaactg cgtatccttt tctttgcttg agaacgactg
     2401 ccgccttgcg agcgtcaatg ccaccctgag aatgcccgat gatgtttaca taagaagcgc
     2461 ctacgcttgt catatagctt tgaatcttat ccgccagttg cgttccgcgg acttctgaac
     2521 tctgaaaagg ctgcacggat gccgcataag ccttctggct ggaattcaac ccgccattgc
     2581 agagtacttc cagaaactca tcgcagggat ctcccacgaa gttcccgttg ttgtccccga
     2641 aatagaacgc ccccagaata ttgtcccagc cagccagacc gtgcgcgaac acaaccggat
     2701 tggctgtctt gcgtactgtg gcattcgccg atgtcatgat cccggtcaaa ctcagagcca
     2761 gaccgaaaac aatggctttc ttcattctat tcctcctgtg cattcgcacc tggcactaca
     2821 tgtgcataga tcgtaccatg caaggcactc acagatacga atggttgcgc ggtgcaccag
     2881 tcgcaggagc gactaacgtt cacgcgaaaa tcgctttcac cgttggccaa agtgattagt
     2941 gcgacgcaaa aaataaaatc ttcgtcttcg cacgcgcgcg aaagaaaaag ggttgatttc
     3001 caatattcgg gattccaaca tttcggaaat tccagatctt tggaacgcgg gggaagcatg
     3061 cggcagagta cgatggcggg gctgatcctg cttgcagggt tatttgtgct tccacgcgct
     3121 gcaagcgctc aaatcaagcc cagcgttgaa attctagaag acccgaccgc aagtctaaca
     3181 tcgcaaactg cgaacatttc aaaaaattgg aaacacttcg gaagcgcaac acccaacctg
     3241 gggtattcgc gttccaccca ctgggtccgg tttgatctgg aagcaccggc ggccggagaa
     3301 tacatcattg aagttgccta tccgctgata gacctcctcg agtgcaccat tctgcgagcc
     3361 aattcagaga gcgtagaaac ccaggggcgc ttgagatcgc aagcggatcg actcccattg
     3421 tttcatttaa gtctggcaga aaaagaaaaa gtgcgagtga tcatgcgcgt acagagtgat
     3481 gattccatca cgttcccggt tcgcatctat acgccgcagg agcacgagca cgaacagaat
     3541 acacgtcagt ttctttttgg actgtactac ggcgtcattg ccgtgatgat tctatacaat
     3601 ctattcctgt attacttcgt gcgcgatcag gcatatttat actacgtact gactctggtg
     3661 ctgctgcatg gccttttcca gctcgctctc aacggctttg cgcgaatgtt ccttggatct
     3721 gagctgctct ggtggaaaaa aggcgccatc gtcttcttcc attctgctgg agtgcttgca
     3781 gccatcctct tttgtcgcgt gtttctactg attccacgag ttgccaaaaa gtgggactac
     3841 atagccagag cgataattgt gtggggagcc ctcaacatgc tcatttcatt ctttgacctg
     3901 tatcgcctgg tcatactatc cacagtgggg cttggaatcc ttggaatcgt cttcgcgctg
     3961 gcttccggaa tcatcgccat gcgaagggga tatagaccgg ccaggttcta tttgcttgcc
     4021 tggtctgctc ttctctttgg cggatttatc tatgttctca aaatttttgg aatcatcccg
     4081 agctactggc ttacggaata cggagtgcaa attggctctg cagtagaagc aactctctta
     4141 tccatcgctc tgggtgatcg gattacgact ggaaggcagg accaggtcaa agctcgcacc
     4201 gaaatgctgg tgcacaaaag aattgcactt ctggcacaaa cggaactcct caatcacctc
     4261 agccagctgg atagtctgaa gagccagatg cattccatcg aatacgaagg taactctctg
     4321 gctagactgc tcgagaacat actcgaaact cttcgcaaga ttctgtcttt tgataaagga
     4381 ttcattgtaa tcgcggatcg acttcaaaaa acgtatctac gaagcgttgg atctatgcag
     4441 ccggatttct acgagaagat accggacgat cactatctcc acatgctcat caaattgccg
     4501 gatgccctgt ttgaccaggt gaatcggatc gtgcatctgg atcgtcctgt gtttgaaaac
     4561 atacctgaaa atagttcgca ggtcactcag gtaatgaaag cgctggaaga tacagtggct
     4621 gcactgcgcg aaactggctt cagtctgtgt ctgcctcttg cctacgaacg cgaaattttt
     4681 ggatacgtta tgctcagcgc gcgcaagagc ggagaacagt acaccaattc agaacttcac
     4741 ctgattgata cattccgccc ttccatcgcc atggccgtgc gaaacgcagt gctctatgaa
     4801 gaagtagttt tgttgcgcgg tcgcgcagaa caaaaggcgg ccaagctttc agatgtgatc
     4861 atcgacatga aagacaccac cagatttgat ctcaaagaaa aagccctcgt ctatattagc
     4921 cacccgatgg ccgagatcta ccagactgtg cgaaagtatg cagataagcc gcagcctgta
     4981 ctcatttcgg gagaaaccgg aacaggcaaa gaactgattg cgcagaccat ccacgaagtg
     5041 ggaggccttt ctagagagcc gttcattcct gtaaactgcg ctgccatccc cgcatctctc
     5101 tgggaaagtg aaatctttgg gcatgaaaaa ggtgccttca cagacgcaaa atcagagaga
     5161 gccgggaaga tcgaacaggc agcagggggc actctattct ttgacgaaat cggcgaaatg
     5221 cctctcgaac tccaggcaaa actactgcgc ttgattcagg aaagaaaata cgaacgagtc
     5281 ggtggaaaga aatctctcga agcacgatgc agatttctct tcgccaccaa ccgtgatctg
     5341 caggtaatgc agaaggcagg aacatttcgc gaagatctct actacagaat cagcgtgttt
     5401 cagatagttc tgcctccttt gcgagaaaga cgagaagaca ttccggtgct cgtgaatttc
     5461 tttattcgca agtatgcggc agaaatgcag gtgcctgttt cgcacataga gaaagctgcc
     5521 atggaatcgc tcacctatta cgactggcca ggaaacatcc gcgaactgga gaactgcgtc
     5581 atccagactc tcgtcaatag cacttcagat acaatccggg tagaggacat tccaggacac
     5641 ctcagttcgc gaggacaggt ggtaagcaaa gaggctcttc gcacgtggga tcctgatatg
     5701 gatttccgcg aactagacgc aatggtcagc gactacacaa gacaggtcat gattgctgcc
     5761 atccagagat gcgcgggaaa caaacaggaa gcggccaaac tactcggtgt gaagagagcc
     5821 acgttctact acagactcaa agaactggga attcaatgaa tcagacgtct tttccagcga
     5881 tcaaatcacg gtagaaaagc gcagaatcct tgggaattcg aacctgggtt tcgtagtcca
     5941 cgtgcaccag tccgaatctt gggcgatagc caaatgccca ttcgaaattg tccaggaagc
     6001 tccatacaaa ataaccgtga acgggagatc cgtctctctt cgcacgaatg acctctccga
     6061 tgtatgcacg aagaaatgcg attctctttt cgtcgtggac gcgcccatct tgcacttgat
     6121 cgggaaacgc ggctccgttt tccgtaacgt agatcttctt gatttccggg taggatccga
     6181 actttcgcag aagctcgtag atcccctgag gatatacttc ccaccccatt tcagtttcag
     6241 gaacgcgtct cttctttgca gacaccggcc tggcccagac gtacggcatc aggatcgatc
     6301 gccttacaac atcacgcgta tagttctgaa ttccaatgaa atcaaaatcg catttcagtt
     6361 tggcatcgtc gcctgatttc atgtgcttct ggatcttctt caaagatggg actgtatccc
     6421 acggatatcc tctcccacag gcaggatcga gaaacaaccg attgatcaca gcatcaaaac
     6481 gatcccgggc cgcctgatga cgcggagcaa atgaccaggc ttccgcatgc gtcatggaat
     6541 aagtactgcc tacttgcgct cctggacaat t
//
LOCUS       DAORFF010000170         6546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6083_length_6546_cov_3.539208, whole genome shotgun sequence.
ACCESSION   DAORFF010000170 DAORFF010000000
VERSION     DAORFF010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6083_length_6546_cov_3.539208"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PKK76_08385"
     CDS             complement(<1..1067)
                     /locus_tag="PKK76_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68530.1"
                     /translation="MTDYAELHSHLYGCLTLDDLRYLAGRNKPRWEIFLKSYESAYGR
                     SADIQDLMAPESDSRLRSYYHFKGPPDFPKFQSAFDLIIALSSTEPGELFEVASRVCA
                     REPAVHAEYRQLFSPMISVSEFEEKTGALAEAMEAACKNHPGKTFRLVMSLPRNPVTG
                     PVLFESVRTLKRAGKNIVGIDFCAQEEGYPPSLLSDLLSAIKSAEERYSILYHVGESF
                     RDKSVESAVRWVVEAALAGAHRLGHAAALGMDPDVFRGSTREELASERLDQIDFEIKN
                     YDSIREFILARPAPGSAAAMVPNKDQLSQEARDIRASGKKVVSVFYSPERTEFLRAFQ
                     DWAMSQVAATGAVVECCPASN"
     gene            complement(1060..1263)
                     /locus_tag="PKK76_08390"
     CDS             complement(1060..1263)
                     /locus_tag="PKK76_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68531.1"
                     /translation="MNPEDLREFYITSEFLTRLSRRARYWSRDFILEQMDHFRSRHPD
                     YPELIDVLQLEIDIRDKKELQVD"
     gene            complement(1331..2707)
                     /locus_tag="PKK76_08395"
     CDS             complement(1331..2707)
                     /locus_tag="PKK76_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNL68532.1"
                     /translation="MHLIVFARAPELGRVKSRLAAAIGKDSALGIYETLLDHTLAVAA
                     HFDFSIYLEGESSLILPVLDRAGIREPYLKEQTGENLGDRMIAAILSELDLHDRVILV
                     GSDIPDLSTDVISQCDRLLLENDLIFGPAHDGGFYLLGVHKGADTEEILRSLFSGMEW
                     SHNSVLAELLARARDLEKNVTLGPVLQDLDNASDLANWLSENQGSLKSRIQKTVPDVR
                     VIIPVLNEAENLPVVIRGLTAVFPFRQIICVDNGSTDGSAEIARSLGAHVLESARGYG
                     NACLAGMDWVKNQGGADVILFADADCADKPQDAVSVVGAVLRGADFALVERIPEEEGA
                     LLPHARFGNWLSTSLIALFFGHSYRDLGPLRAIRFASLLSLEMEDRNFGWTVEMQIRA
                     VRRGLKIVEIPGGYRKRLYGASKVSATIMGSIRAGLIILRTVGREVWRDRGASWAKRR
                     KRNHHPSK"
     gene            complement(2708..3577)
                     /locus_tag="PKK76_08400"
     CDS             complement(2708..3577)
                     /locus_tag="PKK76_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68533.1"
                     /translation="MVDETLQTPETPDPQRKKRDPFQNVGPIVSSETVTGRRELATRS
                     DIKTIKKAGIRVHGRKVVTDDVKKLKSLSAAPIIRPDEKISFYVEPVRIEYYIPKESR
                     FAVEMRDLYIPLIDPVVLPEHEVLHAAMEKNRFVDLIEVMNEYPQTISGLLESYHEVL
                     NLYEHVSRAVKQAEATEPEQFRTAFYLMEVLLENEPTVASLEFMGDFVTYNLNWLVRT
                     MNALGVEFRATDRIVSYFIKLRNNYWEEHNLEYDERFEILASLFFDQAYPHRGTSMAE
                     DDFLFEMFDRQRK"
     gene            complement(3567..4862)
                     /locus_tag="PKK76_08405"
     CDS             complement(3567..4862)
                     /locus_tag="PKK76_08405"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNL68534.1"
                     /translation="MRVKVLFCFVLLPLALLASDRTADLLDSRGDSRRIGMWYVVQEK
                     RSDLLKPVAEILLTSEDVRDQKQVLNVFAAYGDDLETALPEWYVYVDHFLKSGRDEDL
                     LIQAMDLGVKWKEYRLMNAFARMAVHPMHRVRLHAFRSMAALRNDQIMPILLGLSNSG
                     RAIERMYALEGMQEYSDSRVAPFAEKLLQDPNRSVRIFAIPGYAAQPGSDGNSYAIAR
                     LFTQEGDEEVRERVIDLIATRKWGQHTTLVAQACSDPSSLVRAAAFRGARVFNLAGSV
                     SRQLEQEPEARLRSAGLDALAAIGNSGGGTGLAHILSSDENPALRLKAAALLGMYRER
                     MGLSSLHYNLRNDSSADVRAEAADALGDIGDPSSAQVLRTVMEDDQEQIAVKSAAMLS
                     FLKLPLADKKSVLLSIVDTIKDRAFARQLQRAALSFNGR"
     gene            complement(4852..5316)
                     /locus_tag="PKK76_08410"
     CDS             complement(4852..5316)
                     /locus_tag="PKK76_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNL68535.1"
                     /translation="MPKPGISKIEQYITFSVGGEDYGVPVLEVLEIVRIENLIKVPHG
                     RDYLLGMMDIRGQVLPVIDLKTKLQMEAMDSHPERAIIMQIGARKIGLGVDSVSHVRS
                     FSSDNIDEGPPVLRSGSSRHIIGVGKSGDQFIVLMSLNKIFSPEEVEQFFAR"
     gene            complement(5347..6072)
                     /locus_tag="PKK76_08415"
     CDS             complement(5347..6072)
                     /locus_tag="PKK76_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68536.1"
                     /translation="MMELREQSLAPLWKVKPSRREGYHRTYYTETAEGDSILIDFNNV
                     RQMVRIQVTIESEGGRQYACVIKSGTILQERDLTVGRPMDITSRIAPLRFFIGSIPDD
                     MLRSAIRGSYGIPLNGPSPGMRRARIVHTRKVPRMQLLLRRKRARERRPGTMWQKVFR
                     RIPAEGFQIACVGTFALGYLHNLLGMAQFACLVGFFGLWSGAYDWLVRDKSPFLPRIC
                     CMLGISGYAIWLETQYKIWGIFL"
     gene            6151..6468
                     /locus_tag="PKK76_08420"
     CDS             6151..6468
                     /locus_tag="PKK76_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNL68537.1"
                     /translation="MQDIDTVLGDDIQFRGRLQFKRNLKINGAFRGKIQTGGQLIVGS
                     GAKVEADVEASHITVEGELRGNLQASKSLEIKRNAQVSGDIRTPDLHVESGSRFTGSC
                     IMD"
BASE COUNT         1637 a         1745 c         1581 g         1583 t
ORIGIN      
        1 tggttggatg caggacagca ttccacgaca gcacccgtgg ccgccacctg actcatagcc
       61 caatcttgaa aagcacgcag aaattcagtt ctctcaggtg agtaaaacac agacacaact
      121 ttctttccag atgcccgaat gtctcgagct tcctgcgata gctggtcttt gtttggaacc
      181 atggccgccg cggagcctgg tgcgggacga gcaagaatga actcgcggat cgaatcatag
      241 ttcttaatct caaaatcaat ttgatccaaa cgctcagaag caagttcttc tcgagtgctt
      301 ccgcgaaata catcaggatc catacccagg gcagcagcat gtcccagtct gtgagcgccg
      361 gccagagcgg cctccactac ccatcgcacc gcagactcca ccgatttgtc tctgaaactt
      421 tctcccacgt ggtacaagat cgaataacgt tcctctgctg atttgatcgc ggacagcaga
      481 tcggaaagaa gagaaggagg gtacccttct tcctgagcgc aaaaatcaat ccccacaata
      541 ttctttccag cgcgcttgag cgttctgact gattcaaaca aaacgggacc tgtaactgga
      601 tttctgggca acgacatcac aagcctgaac gtctttccag gatgattctt gcaggcggcc
      661 tccatggctt ctgcaagtgc acctgtcttt tcctcgaatt ctgaaacaga gatcatgggg
      721 gaaaacagtt gtctgtattc tgcgtgaacc gcaggttctc ttgcacagac tctggatgcg
      781 acttcaaaaa gttcacccgg ctccgtagaa gaaagagcaa tgatcaggtc aaatgcagat
      841 tgaaactttg gaaaatccgg tggtcctttg aagtgatagt aactgcgcag acgagaatca
      901 gactccgggg ccatcaagtc ctggatatct gcgcttcttc cgtaagcaga ctcataagat
      961 ttgagaaata tttcccagcg cggtttgttg cggccggcca ggtatctgag atcgtctaac
     1021 gttaaacaac catagagatg agagtgtagc tccgcatagt cagtcaactt gcaattcctt
     1081 tttgtcccga atgtctattt cgagctggag tacatctatc agctcagggt aatcaggatg
     1141 gcgagaacga aagtgatcca tttgttctag aataaaatca cgcgaccagt acctggctcg
     1201 cctggaaagg cgagtcagga attcggaggt gatgtaaaat tcgcgcaggt cttccggatt
     1261 cacttgtctt tgtttttctc cttagccttg cgcttctttt cttcgaggag cttcatctca
     1321 tcatgaatgt ctatttcgat ggatggtggt ttctctttct tctcttcgcc catgatgccc
     1381 cccggtcgcg ccatacttcc cgcccaacgg ttcgcaggat gatcaggcct gcccggatcg
     1441 aacccatgat tgtggcggac actttgcttg ccccgtacag ccttttgcga tagcctccgg
     1501 gaatttcaac gattttcagt ccgcgtctga cagcgcgaat ctgcatttcc acagtccagc
     1561 caaagttacg atcctccatt tccaggctca gcaatgaagc aaaacgaatc gcacgaagtg
     1621 ggcctagatc cctgtacgaa tgcccaaaaa acagtgcgat caaagaagta gagagccagt
     1681 ttccaaatcg tgcgtgaggt agaagggcgc cctcttcctc tggaattctt tccacaaggg
     1741 caaaatcggc gcctctaaga accgcgccca ctacactgac tgcatcctgt ggtttgtccg
     1801 cacagtccgc atccgcgaat agaatcacgt ctgcaccacc ctggtttttc acccagtcca
     1861 tgccagcgag gcaggcattc ccataccccc tggcactttc gagcacatga gcgcccagac
     1921 tcctcgcaat ttccgcagat ccatccgtac taccgttgtc cacacagatg atttgtctaa
     1981 acggaaacac agcagtgaga ccccgaatga ctacgggaag attctctgct tcattgagaa
     2041 ccgggatgat gacgcgaacg tccggaacgg ttttctgtat ccggcttttc agagatccct
     2101 gattctctga aagccagttt gccagatcac tcgcattgtc cagatcttgc aacactggac
     2161 ccagtgttac gtttttttcc agatccctgg cccgcgccaa aagttcagct agtacactgt
     2221 tatgcgacca ctccatcccg gaaaacaggc tgcgcagaat ctcttctgta tccgctcctt
     2281 tgtgcactcc gagcaaatag aatccgccgt catgagcagg cccgaatatc agatcatttt
     2341 caaggagcaa acgatcgcac tgagaaatca catcggtaga aagatcagga atgtcagagc
     2401 caaccagaat cacacgatcg tgcaggtcga gttcagatag aatggccgca atcattctat
     2461 ctcccagatt ctcacccgtt tgctctttta aatagggctc gcgaatgccc gccctgtcga
     2521 gaactggcag aatgagagaa gactctccct ccagataaat ggagaaatca aaatgtgcgg
     2581 caaccgcaag tgtgtgatcc agaagagttt cgtagattcc tagggcagag tcttttccga
     2641 tcgcggccgc cagtctggat tttacgcgcc cgagttccgg agcgcgcgca aacacaatca
     2701 gatgcaatta ctttctctgg cgatcgaaca tttcaaaaag gaaatcgtct tctgccatac
     2761 tggttccgcg atgaggataa gcctgatcaa agaagagaga ggccagaatt tcaaatcgtt
     2821 catcgtattc tagattgtgc tcttcccagt aattgttgcg aagcttgatg aaataggaaa
     2881 cgattctatc cgtagcgcga aactcaacac ccagagcatt catggttcgc acaagccagt
     2941 tgagattgta agtaacaaag tcccccataa actcaaggga cgcaaccgtt ggctcatttt
     3001 ccaggaggac ttccatgagg taaaaagccg tgcgaaattg ctctggctct gttgcttccg
     3061 cctgcttgac tgctctggaa acatgttcgt atagatttaa tacttcgtgg taggattcaa
     3121 gcaaaccgga aatcgtctgc ggatattcat tcatcacttc aatgagatct acaaaacgat
     3181 tcttctccat ggcggcatgg agcacttcat gctccggcaa aaccacagga tcaatgagcg
     3241 gaatatagag gtctcgcatt tcgacggcga agcgcgactc tttgggtatg tagtattcaa
     3301 tgcggacagg ctccacgtag aagctgatct tttcatccgg acgaatgatg ggagcggcag
     3361 agagtgattt gagtttcttt acatcgtccg tgacgacttt tcttccgtgg acacggatcc
     3421 ctgctttctt tatggtcttg atatcggagc gagtggccag ctctcgcctt ccagtgaccg
     3481 tctcagagga tacgatgggc cctacattct ggaatggatc tctctttttc ctctgaggat
     3541 ctggcgtttc cggtgtctgt agtgtttcat ctaccattga aagaaagagc cgctctctgc
     3601 aattgccgcg caaaagcccg gtccttgatc gtatctacga tcgaaagcag cacgctcttt
     3661 ttatcggcca gtggaagctt caaaaacgac agcatggcgg cgctttttac agcgatttgt
     3721 tcctgatcgt cttccatcac ggttctcaga acctgcgcag aagaaggatc tccaatgtcc
     3781 cccagagcat ccgccgcttc cgcgcgtacg tctgcggagg aatcgtttcg aaggttataa
     3841 tgcaaagaag aaagacccat cctttcgcgg tacatgccaa gcagtgcggc cgccttgagc
     3901 ctgagtgccg gattctcatc ggaggaaaga atgtgtgcca ggccagttcc gccgcctgaa
     3961 tttccaatcg cggcaagagc atccaggcca gcggacctca ggcgcgcttc tggttcttgc
     4021 tccagctgtc tggaaacgga tccggccaga ttgaaaactc gcgcaccgcg gaaagcggcc
     4081 gctcgaacca gagaagatgg atccgaacaa gcctgagcta ccagagttgt atgctggccc
     4141 catttcctgg tggcgatcag atcgatcact ctctctctga cttcttcgtc cccttcctgg
     4201 gtgaagagcc tggcgatcgc gtacgaattg ccgtccgaac ctggctgcgc tgcataacct
     4261 ggaatggcaa agatgcgcac actgcgattt ggatcctgaa gcaacttctc cgcgaaagga
     4321 gctaccctgc tatctgaata ttcctgcatt ccctcgagag cgtacattct ttcaatggcg
     4381 cgacctgaat tggaaagccc gagcagaatg ggcatgatct gatcatttcg caatgctgcc
     4441 atggagcgaa acgcgtgaag ccgaacccgg tgcattggat gcacagccat tcttgcaaac
     4501 gcattcatca gtcgatattc tttccatttt acacccaggt ccatagcctg aatcaaaaga
     4561 tcttcatctc ttccggattt cagaaaatga tctacatata cataccattc aggaagagct
     4621 gtttccagat catctccgta cgcggcaaac acattgagaa cttgtttctg atcacggaca
     4681 tcttcgctgg taagtaggat ttctgcgact ggcttgagta aatcgcttct cttttcttga
     4741 accacatacc acattccaat cctgcggctg tctccccggc tgtccaggag gtctgcagtt
     4801 ctatcggagg caagtaacgc gagtggaaga agaacaaaac agaaaaggac tttaacgcgc
     4861 aaaaaactgc tccacttctt ctggtgagaa tatcttgttc agactcataa gaacgatgaa
     4921 ctgatctccg ctttttccca caccgatgat gtgcctggag ctgccgctgc gaagtactgg
     4981 aggaccttca tctatgttgt ctgaagaaaa acttctcacg tgcgaaacag aatcaacccc
     5041 gaggccaatc tttcttgcac cgatctgcat gatgatggct cgctctggat gcgaatccat
     5101 tgcttccatt tgtaattttg ttttaagatc aatgacggga agaacttgtc cgcgaatgtc
     5161 catcattccc aggaggtaat ctctcccgtg tggaactttg attagatttt caatgcgaac
     5221 aatctcgagc acttcgagta cgggcacgcc gtaatcttct ccgccaacgg agaatgtgat
     5281 gtactgctcg atcttgctta tgcccggctt tggcatggca cattgtttcc ggcggggctc
     5341 gccttgctac agaaaaatac cccagatttt gtactgtgtt tccagccaga tcgcatagcc
     5401 ggagattcca agcatgcagc aaatcctggg aagaaacgga ctcttgtccc gcacgagcca
     5461 gtcgtatgcg ccagaccaca gcccgaagaa accaaccaga catgcgaact gagccatgcc
     5521 caggagattg tgcaaatacc cgagtgcgaa agttcccaca catgcaatct gaaagccctc
     5581 tgccgggatg cgacgaaaga ccttctgcca catggtgcca ggcctgcgct cgcgggcgcg
     5641 cttccgcctg agaagaagct gcattctcgg aacctttcgc gtgtgcacta tacgagcgcg
     5701 acgcatcccg ggactcgggc cattcagtgg gatgccatag ctgccacgaa tggcggagcg
     5761 aagcatgtcg tctggaatcg atccaatgaa gaagcgcaac ggcgcaattc tgctggtgat
     5821 gtccatggga cgacccacag tcaaatcgcg ctcctggaga atggtgccgg atttaatgac
     5881 gcaggcgtac tggcgaccgc cttctgattc gatggttacc tggatgcgga ccatctggcg
     5941 gacattatta aagtcaatga gaatggaatc gccttctgcg gtctctgtgt agtaagtcct
     6001 gtgatatcct tctctgcgag aaggtttcac tttccacagg ggcgccaggg actgttcgcg
     6061 aagttccatc acattcgaat ttacggcaat tcagcctgag tactcgtgca tttttcttga
     6121 ccctggaggc cctcgcccat tccagagagt atgcaggata tcgacacagt tctcggagat
     6181 gatatccagt tccgaggacg gctgcaattc aaacgcaacc tgaagatcaa cggcgcgttt
     6241 cgcggcaaaa ttcaaaccgg aggccagctc atcgtgggat ctggcgcgaa ggtagaagca
     6301 gacgtagaag caagtcacat cacagtagaa ggagagcttc gcggcaatct ccaggcttct
     6361 aaaagccttg agatcaaaag aaacgcgcaa gtatccggtg acattcgcac tccagacctg
     6421 catgtagaat ccggctcccg gttcaccgga agctgcatca tggattgatc aacccttaga
     6481 ggaccaataa agatgaaaaa agcaattgtg ctcgcgatcg tactgttgtc cgcgtgtaaa
     6541 cagggt
//
LOCUS       DAORFF010000171         6507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6141_length_6507_cov_3.653596, whole genome shotgun sequence.
ACCESSION   DAORFF010000171 DAORFF010000000
VERSION     DAORFF010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6141_length_6507_cov_3.653596"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PKK76_08425"
     CDS             complement(<1..699)
                     /locus_tag="PKK76_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68538.1"
                     /translation="MIHPIRALRDMVCLLQRESMSAPVRSVLDFEEKNGARMANLFRY
                     ALAALIAIPIVFAAQNGRELIINLVALSAYLLFTILHTVLLRRRSSSFMVVFNYLAVL
                     YDYVLISGLIVYYSKLVSPGNFAHAAKNPTLLYFLFPLALTVLQFRLRLLIFALICLC
                     TFWWSLIAYGVFTGMPLTNDWNEYLLGPAVILSDAVTRPVPFVGLALLLSYSIYRSIF
                     MIRRIGDLESRRASL"
     gene            complement(696..1961)
                     /locus_tag="PKK76_08430"
     CDS             complement(696..1961)
                     /locus_tag="PKK76_08430"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNL68539.1"
                     /translation="MKEVSIGILGAGNVGRGLIELIARDGEAIAARTGIRLKVAAVFD
                     RSYQKKSEALAGVPASDDPSIVLSNPDVSIVVELLGGLSPAGDLIRSALSAGKAVVTA
                     NKALVSSPAGPELLKLAQDQNLNFGFEAAVAGAIPIIRTLRMHCVSGEVRSIRGILNG
                     TCNYMITRMEEDGLDYDAALRLAQEKGFAEADPTFDVSGQDAAQKLAILAGIALDSYV
                     PVDRLRVEGIESIRPVDLKIARRMGWVIRLLAIARRTDAGELLRVHPAMIQEDHILAS
                     VKEERNAVFVDSTSAGPSLLVGKGAGSHPTATAVLSDVIDLARGGEVRPALFQKTEPE
                     ARADFNYRFYVRVQTHDRPGVLAEIATVFADHDISIASFHQEEGPEPVQLVIVTHAAL
                     ESAMFEAVAEMDRLRAVMLPSVVIRIEDL"
     gene            complement(1958..2221)
                     /locus_tag="PKK76_08435"
     CDS             complement(1958..2221)
                     /locus_tag="PKK76_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68540.1"
                     /translation="MAARRDDEHELDDITLLNVHRYPDSVPASIVASVRNSKAAQKRL
                     EELLKGMAGGRRSRVEVPVPEGQQSPAPEASSSLRDLIKKFIP"
     gene            2326..3567
                     /locus_tag="PKK76_08440"
     CDS             2326..3567
                     /locus_tag="PKK76_08440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HNL68541.1"
                     /translation="MRTLLLVLCAGLAAIHCKKPATVISPDEANFQKKLQESLLTAKP
                     GSVIELPAGTFHLDKGMTLSEKGVTIRGAGMDKTILSFKGQTEGAAGIMVSQGDFLIE
                     DLTIQDTTKDAIKVTGAKNVTFRRIKTEWTEGAKETNGAYGIYPVQCENVLIEESVAI
                     GASDAGIYVGQSKNIIVRRNRAEMNVAGIEIENSTFADVYENTSTNNTGGILVFDLPD
                     LPVQGGKNTRIFKNQIVSNNLKNFAPSGNIVAMVPTGTGFMIMANDNIEAFENTIKDN
                     ITTNVLIVSYLVTEKPIKDKNYDPYPEAIHVHNNTITGGGVDPTGGNSLASRVAVAGL
                     RAKLGKPFPQVLWDGIVNKARFPDRKLTPDAQICIRDNGNADFGNFDAANNMSDIKRD
                     LAVHNCSHPALSPNRIPGVRD"
     gene            3564..4655
                     /locus_tag="PKK76_08445"
     CDS             3564..4655
                     /locus_tag="PKK76_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SO2930 family diheme c-type cytochrome"
                     /protein_id="HNL68542.1"
                     /translation="MKAIALVLLVAAFSATGCKKSDGVQFYDDNFPAKLSDWKLFVRS
                     GENLTMAAGLIPYDLNSPLFSDYARKFRTVYVPPGKSAEYTKIGVLNLPVGTILSKTF
                     YYPRKAGAVESSDFPSMKENHVIETRLLVHTSDGWVGLPYIWNKEGTEATLEITGGDS
                     NVQSFDMDGKPIAFKYSVPNQGQCSGCHSARRGEDKVMTPIGPKARQLNKVYAYKGGP
                     ENQIDHWKKSGILNVPADAVVVRNAVWNDPQTGTVEERARAYLDVNCAHCHIEYGPAN
                     QSGLILSLENQEPHRFGVCKSPTAAGRASANMDYDIVPGNPDSSILIHRMTSTEAGIR
                     MPELARSLLHKEGLSLVREWVKQMKGNCN"
     gene            complement(4661..5218)
                     /locus_tag="PKK76_08450"
     CDS             complement(4661..5218)
                     /locus_tag="PKK76_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNL68543.1"
                     /translation="MRHTMERFRACQPTPGLQRKKIALCPLNVPLIEMAGKRTADEQH
                     IAELERTLEAIRRKDRLMTESQSTVSRILLNAPVMIYVYDLVDKRNVYINREATDFLG
                     YSSDQIRDLGPDLLSTLIHPEDLPRAIAHHNRLLGATGPLKLVYRMKNAKGEWRNFLS
                     CDTPFETEPGEQTRFILGCVQEMPD"
     gene            5217..6479
                     /locus_tag="PKK76_08455"
     CDS             5217..6479
                     /locus_tag="PKK76_08455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL68544.1"
                     /translation="MLNPFRPAEHQPLLPVEEQNRRIAPLRWQILEATFLGYTFYYFT
                     RNNLPIVSLEAASDLTYSSAQMGLLMGATGLAYGIGKFILGALSDRSNPRAFMPAGLL
                     LSAALNFLFGAASNFWIHLGLWTLNGFVQGAGWPPCGRSLGHWYSKSERGTVFGFWNI
                     AHNIGGGLIGPLAAAAASAFGWRSAFYIPGILAVLAAVYLMIRLRDTPQSVGLRSIEA
                     FPPMSNAKYLIALIASVLTGNAIAGAVMYTLLPYEQLPAAVTMLNLVLIPACFWVIGM
                     LPGNRASPPSRLSAAIPAHDVSLEKELSLWEILWNNVLTNKIIWLFAIANLFVYVVRY
                     SLIDWGPTYLKQAKGASMQTGGFSTLAFEASAIVSTLLIGWISDKSGGRRGMVSLICM
                     IPVFLAFLGMIVMPPGFLWAHIVLFGVV"
BASE COUNT         1725 a         1661 c         1642 g         1479 t
ORIGIN      
        1 cagagatgca cggcgactct ccagatctcc aattctacga atcataaaaa tagatctgta
       61 aatgctataa gacaatagaa gcgccagccc aacaaatgga acaggtctgg tgactgcgtc
      121 agacagaatc acagccgggc ccagaaggta ctcgttccag tcgtttgtaa gtggcatccc
      181 ggtgaacaca ccgtaggcga tgagagacca ccagaatgtg caaaggcaga tcagtgcgaa
      241 gatcagaagc ctgaggcgaa actgcaaaac agtcagagca agaggaaaga gaaagtaaag
      301 cagtgttgga ttcttcgctg catgcgcgaa attcccagga ctcacaagtt tgctgtagta
      361 aacgatgagg ccgctaatga gcacgtaatc ataaaggact gccaggtagt taaaaaccac
      421 catgaaggag ctcgatcttc tccgaagcaa cacggtatga agaatcgtaa agagaagata
      481 tgcagaaagt gcgaccaggt tgatgatcag ttctctacca ttctgtgctg caaatacaat
      541 gggaatcgca atgagggcgg ccagggcata gcgaaacaga tttgccatgc gcgcgccatt
      601 cttctcctcg aaatccagaa cagagcgaac cggcgcggac atagactcgc gctgaaggag
      661 gcaaaccatg tcccgaagtg cacgaattgg atgaatcata agtcttcgat tcgaatcact
      721 acagaaggaa gcataacagc gcgaaggcga tccatctctg cgactgcctc aaacatggca
      781 ctttcgagtg cggcatgagt gacaatcaca agctgtactg gctctggacc ttcttcctgg
      841 tgaaaagatg ctatggaaat gtcatgatcc gcaaacacgg ttgcgatttc cgcaagtacg
      901 cccggacgat catgcgtttg cacacgcaca taaaaacgat aattgaaatc cgcccgcgct
      961 tctggctccg ttttttgaaa cagagcaggc ctcacttctc ccccgcgcgc cagatcaatc
     1021 acgtcactga gaactgcggt ggcagttgga tgcgatcctg caccctttcc tacaagcaat
     1081 gatggaccag cagaagtcga atccacaaac actgcatttc tttcttcttt gacggaagcg
     1141 aggatgtgat cctcttgaat catggccgga tgaacgcgca gcaattctcc tgcgtctgtg
     1201 cgacgcgcaa tggcaagaag tcgaatcacc cagcccatgc gtcgtgcgat cttcaaatcc
     1261 acggggcgaa tcgactcaat tccctctacg cgaagacgat ccacgggaac ataggaatcc
     1321 agagcaatgc ccgcaaggat tgcaagcttc tgtgctgcgt cctgaccgct tacatcaaag
     1381 gttggatccg cttccgcaaa tcccttctcc tgagcaaggc gaagtgcagc atcgtaatcc
     1441 agtccgtcct cttccatgcg agtgatcatg tagttgcatg tgccattgag aattccgcga
     1501 atagagcgca cttccccgga aacacaatgc atgcgaagag tgcgaatgat cggaatggca
     1561 cccgcgacag cagcctcgaa accaaaattc aagttttgat cctgcgcgag tttgagtagc
     1621 tcagggcctg caggcgaaga gacgagcgcc ttatttgcag tcacaacagc cttacccgcg
     1681 ctcagtgcag agcgaatcaa atcgcctgcg ggggacagac cacctaacag ctcgactacg
     1741 atggatacat cggggttcga tagtacaatc gaaggatcgt cggaagcagg aacacccgcg
     1801 agtgcctcag actttttctg atacgagcgg tcaaatacag cggcgacttt caggcgaatg
     1861 ccagtgcgcg cggcgattgc ctctccgtct cgcgcgatca attcaatgag gccgcggcca
     1921 acgttgcccg ccccgaggat tcctatgctt acttctttca aggaatgaac ttctttatca
     1981 aatcgcgcag gctcgaacta gcttctggtg cgggtgattg ctgtccttct ggaaccggaa
     2041 cttccacacg actgcggcga ccgcctgcca tccctttgag aagttcttcg agtctcttct
     2101 gcgctgcctt ggaattgcgc acggaggcaa cgatggatgc tggaacagaa tctggataac
     2161 gatgtacgtt caggagcgta atatcgtcga gttcatgttc gtcgtctctg cgggcagcca
     2221 tacctgaaaa gaaagggcgg cacccggcag gtcaagtgaa aacgggaaaa caacttgaac
     2281 catctccgga ggcccccagg ctctgcccaa aaccaggggg gaaccatgcg aactttgcta
     2341 ctcgtacttt gtgcaggcct tgcggcaatt cattgcaaaa aaccggccac cgtcatctca
     2401 cccgacgaag ccaattttca gaagaaactc caggaaagct tactgactgc gaaaccaggg
     2461 tcagtcatag aacttccggc aggaactttc cacctggata agggaatgac tctctcagaa
     2521 aaaggagtca caattcgcgg ggcgggaatg gacaagacca tcctttcatt caagggacaa
     2581 acagaaggcg cagctggaat catggtttcg cagggcgact tcctgatcga agatctgacc
     2641 attcaagaca ccaccaagga cgccatcaaa gtaaccggcg caaagaatgt cacgtttcgc
     2701 agaatcaaga cagaatggac agaaggcgcg aaagagacaa acggagccta cggcatctat
     2761 ccagtgcagt gcgaaaacgt gctcattgaa gaatcagttg cgattggtgc ttccgacgcg
     2821 ggcatttacg taggccagtc aaagaacatc atcgtgcgac gcaatcgcgc agaaatgaat
     2881 gtggcgggaa ttgaaatcga aaattcaact ttcgcagacg tgtacgaaaa cacttctacg
     2941 aacaacacag gtggaatcct ggtgttcgat ctgccagatc tacctgtgca gggcggaaag
     3001 aatacgcgca tcttcaagaa ccaaatcgtg tccaacaatc tgaagaactt cgctccatcc
     3061 gggaacattg tagccatggt tccaaccggt actggattca tgatcatggc gaacgacaac
     3121 atagaagcat tcgagaacac aatcaaagac aacatcacaa caaacgttct gatcgttagc
     3181 tacctcgtta cagaaaaacc aatcaaagac aaaaattacg atccataccc ggaagccatt
     3241 catgttcata acaacacgat cacgggcggt ggagtcgatc ctaccggtgg aaacagcctt
     3301 gcatccagag ttgcagtcgc aggtcttcgt gcgaagctag gcaaaccatt ccctcaggtt
     3361 ctctgggatg gaattgtaaa taaagccagg tttccagatc gcaaacttac accggatgca
     3421 cagatctgca ttcgcgacaa cggcaatgcc gatttcggaa attttgatgc ggcaaacaac
     3481 atgagcgata tcaaacgaga tcttgcagtc cacaactgct ctcatcctgc gctttccccc
     3541 aatcgcattc cgggagtgcg tgattgaaag cgatcgctct ggtgctgctg gtggcggcgt
     3601 tttccgcgac tggatgcaag aaatcagatg gtgttcagtt ctacgatgac aacttccccg
     3661 cgaagttgtc agactggaaa ctcttcgttc gatccggaga gaatctgact atggcagcgg
     3721 gcctcattcc gtacgatcta aacagcccac ttttttcaga ctatgcgcgc aaatttcgca
     3781 cagtctacgt gccgcctgga aagtcagccg aatacacaaa gatcggtgtt ctgaatttgc
     3841 ccgtaggaac cattctctcc aaaacattct attaccccag aaaggcaggt gcagtggaat
     3901 cgtctgactt tccgtcgatg aaagagaatc atgtaattga aaccagattg ctcgtgcaca
     3961 catcagacgg atgggtcggt ctgccctata tctggaacaa ggaaggaacg gaagcaacgc
     4021 tcgagatcac aggcggagac tcaaacgttc agtctttcga tatggacgga aaaccaatcg
     4081 catttaagta ttcagttcca aaccagggac agtgctctgg ctgtcattca gccagacgcg
     4141 gcgaagacaa agtcatgacg ccgattggac caaaagcgcg tcagctcaac aaagtctacg
     4201 cgtacaaagg tggtccagaa aaccagatcg atcattggaa gaagtctgga attctcaatg
     4261 tcccggcaga tgcagttgtt gtgcgaaatg cagtatggaa tgatcctcaa acgggaacag
     4321 tcgaagaaag agcacgcgcg tatctcgacg ttaactgcgc gcattgccac atagaatatg
     4381 ggccagcgaa tcaatccggt ttgattttga gtttggaaaa tcaggaacca cacagattcg
     4441 gtgtttgcaa atcgccaact gcagccggtc gcgcgtctgc gaatatggac tatgacattg
     4501 tgcctggaaa tccggattct tctattctga ttcatcgcat gacttcaacg gaggctggaa
     4561 tcagaatgcc cgaactggca agaagccttc tccacaaaga gggattgagc ctggtacgcg
     4621 aatgggtgaa gcagatgaaa ggaaactgca actagagaag ttagtctggc atttcctgaa
     4681 cgcatccaag aatgaaccgg gtctgttctc ccggttctgt ttcgaaagga gtatcgcagg
     4741 ataggaaatt cctccattct ccttttgcgt tcttcatgcg atacacgagt ttcaaagggc
     4801 ccgtggcacc gagcaaacga ttgtgatgcg caatcgcgcg aggaagatct tccggatgaa
     4861 tcagagtgga aagaaggtcc ggcccgaggt cgcgaatttg atccgatgaa tatcctagaa
     4921 agtccgtggc ttcgcggttg atgtaaacgt ttctcttatc cactagatcg taaacgtaaa
     4981 tcatcacagg cgcattcaat agaattctgc tgaccgtaga ctggctctcg gtcatcaaac
     5041 gatccttgcg cctgatcgct tcgagagttc tttcgagctc agcgatgtgc tgctcgtccg
     5101 ctgttcgttt ccccgccatc tctatcaatg gaacattcag cggacaaagt gcaatctttt
     5161 ttctctgaag tccgggcgtt ggttgacatg ctctaaatcg ttccatcgta tgtcgcatgc
     5221 tgaatccatt tcgtccggcc gagcaccagc ctcttctgcc ggtggaagag cagaatcgca
     5281 gaattgcccc tcttcgctgg cagattctag aagcgacgtt cctgggttat actttctatt
     5341 actttaccag aaacaacctg cccatcgtct ctcttgaagc cgcttccgat ctcacttaca
     5401 gttcggcgca aatgggattg ctcatgggtg cgaccggtct cgcatacgga attggaaaat
     5461 tcatcctggg cgccctctcc gacagatcca atccaagggc attcatgccg gcaggcctcc
     5521 ttctatcagc tgcactgaat tttttgtttg gagcggcgtc taacttctgg attcacctcg
     5581 gactctggac tctcaatgga tttgttcagg gagcaggctg gcctccgtgc ggacggtccc
     5641 tcggacactg gtattctaaa tcggaacgcg gaaccgtttt tggattctgg aacatagcgc
     5701 acaacatcgg aggcggtttg attggaccac ttgctgccgc tgccgcatct gcgtttggat
     5761 ggagatctgc gttttatatc ccggggatcc tcgccgttct tgccgcagtg tatctgatga
     5821 ttcgactgcg agacactccg caaagcgtgg gcctgagatc catcgaagcg tttcctccta
     5881 tgtcgaatgc gaagtacctc atcgcactga ttgcatctgt tctgacagga aatgcaatcg
     5941 caggcgcggt catgtatact ctgcttccct acgaacagct tccggcagcg gttactatgt
     6001 tgaatctggt tctcatacct gcttgcttct gggtgatcgg aatgctaccg ggcaatcgcg
     6061 ccagtccacc ttcgcgcttg tctgcggcga ttccagcgca tgacgtctct cttgagaagg
     6121 aactgtcctt gtgggaaata ttgtggaaca acgtactgac gaacaaaatc atctggctat
     6181 tcgcgattgc aaatctgttt gtatacgttg tgagatacag cctcatagac tggggaccta
     6241 cctatctcaa gcaggcgaaa ggcgcgagca tgcagacagg tggatttagc actcttgcat
     6301 ttgaagcaag cgccatcgtc agcactctgt tgattggatg gatttcagac aagtcaggcg
     6361 gaagacgagg catggtgagt ttgatatgca tgattccggt gttcctggcc ttcctcggga
     6421 tgattgtgat gcctccggga tttctctggg cgcacattgt tttgtttgga gtagtctaac
     6481 tatgttttga tgaacaagtc agaaaat
//
LOCUS       DAORFF010000172         6444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6259_length_6444_cov_4.153858, whole genome shotgun sequence.
ACCESSION   DAORFF010000172 DAORFF010000000
VERSION     DAORFF010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6259_length_6444_cov_4.153858"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PKK76_08460"
     CDS             <1..1164
                     /locus_tag="PKK76_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNL68545.1"
                     /translation="TITAVAFTSVPTLAKDLAVARPTFLLSVPRVWESFYNRVMDNVK
                     KASSFNRGMFMFSSWTAMHFSSYKDILLGLQYRLKKEFIGITLLRKLGALIALIFLFV
                     PNLIAQALLGRVRQILGGRVDFAISGAGALPEYIDRFFYSIGVPIVETYGMTETVGVS
                     ARRKYPGTVIGTVGATFPGVEIKLVDDKGNIVTEPNVKGVAYHRGPHIMLGYYKEPEK
                     TRAVLSEDGWLNSGDILVWTVGGQLKFAGRAKDTIVLLGGENVEPQPIEDALAQSDLI
                     AQIVVVGQDKKTLGALIVPNAESVKKALMLEGKQAPESIEKWNEDPAIVSMFKNEIKA
                     LNSDKAGFKNFEKVTAFTLLSKEFQPGDELTQTLKVKRNVVFDKYQKEIERMY"
     gene            1164..2069
                     /locus_tag="PKK76_08465"
     CDS             1164..2069
                     /locus_tag="PKK76_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL68546.1"
                     /translation="MDSQVLIVDDDPLIVDLVLQIFADSSHRVFKAANATEAENLVHS
                     ENISLIILDWEMPGRSGIDLLRDLKRDRKYKYLPVIMLTSRSDEADVKAGIGAGAFYY
                     LTKPFDKRILMALVDSATNDFKAIRIREQPDPSVIYSCARSSVFEFRTPEEAVELAKW
                     LARMCPDAQGAQLGLQEILVNAIEHGNLGIAYEEKTQLVQKQALYAEMRRRLLIEPYA
                     SRRARVTFEKRESSYSFFIEDQGEGFDFDMFLSLTPERAFSEHGRGIAIARYLCFDEL
                     DYVAPGNKVHAVIRQKHESRGQSFA"
     gene            complement(2046..2684)
                     /gene="pdxH"
                     /locus_tag="PKK76_08470"
     CDS             complement(2046..2684)
                     /gene="pdxH"
                     /locus_tag="PKK76_08470"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002150650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNL68547.1"
                     /translation="MPIDPSSLRKEYTAAGLLEESAGFDPFALFDRWFHEAVAAEVRE
                     PTAMTLATSNRQGSPSARIVLMKGYDERGIIFYTNYESHKANDLAENPQAALLFFWAE
                     LERQVRIEGSITKTSRKESDEYFQSRPLESRIGAHASNQSKKIESREVLTQKVREIEA
                     KFGSSVPIPDQWGGYILNPKKMEFWQGRPARLHDRILFSKSDAGWLRERLSP"
     gene            2767..4233
                     /gene="purF"
                     /locus_tag="PKK76_08475"
     CDS             2767..4233
                     /gene="purF"
                     /locus_tag="PKK76_08475"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017213797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNL68548.1"
                     /translation="MPIRSDLRDRFQDDHQIPREECGIYGISGCKEAANFTYLGLYSL
                     QHRGQESAGIVTSDGQRLFRYAGMGQVAQVFTEEKLRDLSGSSAIGHNRYSTTGASFL
                     RNAQPIRVESNLGGFALAHNGNLVNAWSLRSELERSGSIFQTTIDSEVIVHLMARSGK
                     TDFLDALIYALRQVEGAYSLLILTRDALYAVRDPNGFRPLVMGRRQDGSIVFASETCA
                     FDITDTTYERDVHPGEIVCVDGKGITSYHPFEKRAEALCIFEHIYFSRPDSRIFEQSV
                     YDVRKRLGQVLARAHPCDGDVVVPVPDSSTVAAIGFAEESGIPYTMGLIRSHYIGRTF
                     IEPEQRIRDFGAKIKYNVIRSAIEGKRVVVIDDSIMRGTTSRKLIKMFRRNGAKEVHL
                     RISAPPTRFPCYYGIDIPTRGELIAATHSVEEIIRYLNVDSLGYMSLEEAHSAMMDGT
                     RDRKWCDACFSGTYPLKLDQNDNSNQKDLFPEYLVEER"
     gene            complement(4230..>6444)
                     /locus_tag="PKK76_08480"
     CDS             complement(4230..>6444)
                     /locus_tag="PKK76_08480"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL68549.1"
                     /translation="LAAAIMIVYMIYGIALAAIRKRDGAITVLGGASIAGAAGMNDIL
                     VAAGVIPGDFLTPVGFFVLIFSQATVLARVSAKAFATAEHLSQNLREEVRQQTDELRQ
                     ASEKLLQADKEKTAFFQNISHELRTPLTLIYGPVVDAVRRKEGIATEELGTVLSNARR
                     LLRLVNQLLDLQKIAAGKMELRLRSMNLGEMLGSISEAFQPYANGKRIQIETQIDGDI
                     PRVAADPDMIEKCVYNYLSNAVKFTPAGGRVRICAHRKDNGVEVAVHDSGAGISDENQ
                     AGLFTRFGRSEPSLTRDQEGTGLGLSLVRELIALHGGHVSAKSVPGNGASFYFWLPEI
                     SEILPEHDSSVSVMADIELADEVAADDASAHVQASGTTILLVEDNRGMRGYISGILRR
                     SGYTVVETTDGQHGWTAVRSRRPSLIITDLMMPRMSGIDLIANVRADPELKTLPVILL
                     TAKADTRTRQEAHTAGADNYLAKPFNELELLSVVSNLLTLKSTEARLVREIELARKIQ
                     NTLLPAKIPELPGLRIACRYKPMERIGGDLYDFCVYPDGSLGILIADVSGHGIAAAMI
                     ASMTKLAFALYGPTAESPADLLQQINSALLGRTGGNFITAQYAMIPANRKTVTVASAG
                     HPAPLFIRDGETRPMLTRGPMLGILPSIQLMQFTLEIKSGDRLLLYTDGITEVMNAQK
                     QLFEEKRLVDTLTKTANLPLEASMLTLIEEASAFAGTYVFPDDLTLVCFEFDAGI"
BASE COUNT         1540 a         1574 c         1657 g         1673 t
ORIGIN      
        1 accatcacag cagttgcgtt tacttctgtt cctactcttg cgaaggatct cgctgtggca
       61 cgtccaacat tccttctgtc tgtgcctcgc gtgtgggaga gtttctacaa tcgtgttatg
      121 gacaatgtga agaaagcgag cagcttcaat cgcgggatgt tcatgttttc gtcctggact
      181 gcaatgcact tctcttccta caaagacatt cttcttggtc tacagtacag actgaagaaa
      241 gaattcattg ggatcacatt gcttagaaag cttggcgcat taattgcgct catattctta
      301 tttgttccca atctcatcgc gcaggctttg cttggtcgag tcagacagat tctgggtggt
      361 cgagttgatt tcgcgatttc aggtgccggt gcacttccag aatacattga tcggtttttc
      421 tactccattg gcgttccgat tgttgaaacg tatggaatga cggaaacggt cggggtgagc
      481 gcgcgtcgca agtatcctgg cacggtgatt ggtactgtgg gcgctacatt tcctggagta
      541 gaaatcaagc tggtagatga taaaggaaat atagtaacgg aaccaaatgt gaaaggcgta
      601 gcttatcatc gcggtccgca catcatgctt ggctactaca aggagcctga aaaaacgcgc
      661 gcagttcttt cagaagacgg ctggttgaac tccggtgaca tcctggtctg gaccgtggga
      721 gggcagctga agttcgccgg tcgcgccaag gatacgattg tactccttgg tggagaaaac
      781 gtggagccac aaccaatcga agatgcactg gcacagagcg atttgattgc tcagattgtg
      841 gtcgtgggtc aagacaagaa gactctggga gcattgatcg ttcccaatgc agaatcagtg
      901 aaaaaagctc tgatgctcga aggcaagcag gctccggaat ccattgagaa atggaatgaa
      961 gacccggcga ttgtatcgat gttcaaaaac gaaatcaaag ccctcaattc ggacaaggcc
     1021 ggattcaaga actttgagaa ggtcacagca ttcaccttgc tctccaaaga gtttcagcca
     1081 ggagatgagc tgacgcaaac tctgaaagtg aagcgaaacg ttgtatttga caagtatcag
     1141 aaagagattg aacgtatgta ttgatggact cgcaggttct cattgtagat gacgatccgc
     1201 tgattgtgga tctagtctta cagatcttcg ccgattcaag tcaccgtgta ttcaaggcgg
     1261 ccaacgcaac ggaagcagag aacctggtgc attcggaaaa catatcgctc atcatcctgg
     1321 actgggagat gccgggaaga tctggcattg atttattgcg ggatctgaag cgggacagga
     1381 aatacaaata tctcccggtc atcatgctca ccagcagatc agatgaagct gatgtaaagg
     1441 cgggcattgg tgcgggtgca ttttattatc tgaccaaacc tttcgacaag cgaatcctga
     1501 tggcgctcgt agatagcgcg actaacgact tcaaagcaat tcgcatcaga gagcaaccgg
     1561 atccatccgt aatttattcc tgcgcgcgct cttctgtatt tgaatttcgc actccggagg
     1621 aagcggtaga gcttgcgaag tggcttgcgc gcatgtgtcc tgatgctcag ggagcgcaat
     1681 taggcctcca ggaaatcctg gtcaatgcaa ttgaacatgg caacctgggc atcgcatacg
     1741 aagagaagac gcaactcgtg cagaagcagg ctctgtacgc ggagatgcgc cgccggctct
     1801 tgatagaacc gtacgccagt cggcgagcgc gcgttacttt tgaaaagaga gagtccagtt
     1861 attctttctt catagaagat caaggtgaag gctttgattt tgacatgttt ctttctctta
     1921 caccggagcg cgcgttctca gagcacggca gagggatcgc catcgcgcgt tatttgtgtt
     1981 ttgatgaact ggactacgtt gcgcccggga acaaggtgca cgcagtcatt cgacagaagc
     2041 acgaatcaag gggacagtct ttcgcgtaac caacccgcgt cactcttgct aaagagaatt
     2101 cgatcgtgca ggcgagcggg tctgccttgc cagaactcca tttttttcgg attgagtatg
     2161 tatccacccc attgatccgg gataggaaca ctggaaccaa acttcgcttc tatttcgcgt
     2221 accttttgcg taagtacttc gcgactttca atcttcttgc tttgattgga tgcatgcgct
     2281 ccgattctgc tttccagagg tctactctgg aaatactcgt ccgactcctt tctgcttgtc
     2341 tttgtgatgc ttccttcgat tcggacctgt ctttccagct cagcccagaa gaacagaagt
     2401 gcagcctgcg ggttttctgc cagatcgttt gccttgtgtg attcgtagtt tgtatagaag
     2461 ataatccctc tttcgtcata ccctttcatg aggacgatgc gcgcagaggg actgccctgg
     2521 cgatttgagg tggccaacgt catagccgtg ggctcgcgaa cttccgccgc cacagcctcg
     2581 tggaaccatc ggtcaaagag agcaaacgga tcaaatccag cggattcttc gagtaaaccg
     2641 gccgctgtgt actctttgcg gaggctggaa gggtcaatgg gcatggttca acaaaacgtg
     2701 cactttcaag cgttcaatca gaaaaaaaaa catttccccc gtagcccctt gtgaaaacgt
     2761 agctgtatgc ccatccgctc cgatctgcgt gatcggttcc aggacgacca tcagataccg
     2821 cgcgaagagt gcgggatcta tggaatctca ggctgcaagg aagcagccaa cttcacctat
     2881 ctcggtctgt actctctgca gcaccggggc caggaaagtg caggcatcgt aacctcagac
     2941 ggccagagac tgtttcgcta cgctggcatg ggccaggtgg cacaggtatt tacggaagaa
     3001 aaactcagag atctatcggg ttcttccgcc atcggtcaca accgctattc aaccacgggc
     3061 gcgtcttttc taagaaatgc gcaacccatt cgcgtagaat ccaatctggg tggatttgct
     3121 ctcgctcaca acggaaacct ggtcaatgcc tggtcgcttc gcagtgagct tgaaagatca
     3181 ggatcgatct ttcagactac gatcgactca gaagtgattg ttcacctcat ggcgcgcagt
     3241 gggaagactg atttcctgga cgcactcatc tatgcacttc gccaggtgga aggtgcctac
     3301 agtctcttga ttttgacacg cgacgcgctc tatgcggtgc gcgatccaaa cggatttcgt
     3361 cctctcgtca tgggaagaag gcaggacggt tcgatcgttt ttgcatctga aacgtgcgcc
     3421 tttgatatta cagacactac gtatgagcgc gacgtccatc cgggtgaaat cgtatgcgtg
     3481 gatgggaagg gaattaccag ttatcatcct tttgaaaaac gtgcggaagc actctgcata
     3541 ttcgagcaca tctatttttc tcgtcccgat tctcgcattt ttgagcagtc agtctatgat
     3601 gtgagaaagc gtcttggcca ggtccttgca cgcgctcatc cttgcgatgg agatgtggtg
     3661 gtccctgttc ctgattcgtc gacggtagct gccattggtt ttgcggaaga atccggaatt
     3721 ccatacacca tgggactcat tcgatctcat tacattggtc gcaccttcat tgagcccgaa
     3781 cagcgcatcc gcgactttgg tgcgaagatc aagtacaatg tgattcgttc tgcgattgaa
     3841 ggaaaacgcg ttgtggtgat tgacgattcg atcatgcgcg gaaccacatc gcgcaagctc
     3901 atcaaaatgt tcagaagaaa tggcgcaaaa gaagtccatc ttcgaattag cgctccacct
     3961 acccggtttc cctgttacta tggcattgat attcctacaa gaggagagct catcgcagcc
     4021 acgcattctg tggaagagat cattcgatat ttgaatgtag attcactggg atacatgagc
     4081 ctggaagaag ctcattctgc gatgatggac ggcacccgtg atcgcaaatg gtgcgatgcg
     4141 tgtttctcag gtacataccc gctcaagctg gatcaaaacg ataattccaa tcagaaagat
     4201 ttgtttccag agtacctggt agaagaacgc taaatccccg cgtcaaattc aaaacaaacc
     4261 agagtgagat catctggaaa gacatatgtg cccgcaaatg cgcttgcttc ttcgatgaga
     4321 gtgagcatgg acgcttcgag tggaagattt gccgttttcg tgagagtatc taccagtctt
     4381 ttttcttcga acagttgctt ttgagcattc attacttctg tgattccatc tgtataaaga
     4441 agaagtctgt cgcccgattt gatttctaga gtaaactgca tcaattgaat ggacggtaaa
     4501 attccgagca taggtccgcg cgtcagcatg ggtctggttt cgccgtcgcg aatgaataga
     4561 ggagctggat gccctgcaga agccacggtt acagtttttc tgttcgccgg tatcatcgcg
     4621 tattgagcgg tgatgaaatt gcctcccgtt ctgcccagga gtgcgctatt gatttgttgg
     4681 agtaagtccg ccgggctttc tgcggttggt ccgtagagcg cgaaggcaag tttagtcatg
     4741 gatgcaatca tcgccgcggc aattccatgg cctgaaacat cagcgattaa aattcccagg
     4801 cttccatccg gatatacgca gaagtcatac aaatctccgc cgattctttc catgggcttg
     4861 tatctgcatg caatgcgaag gcccgggagt tcaggaattt tcgcaggcaa taaagtgttt
     4921 tgaatcttgc gcgccagttc gatttcgcgc acaagtcttg cctctgtgga tttgagagtg
     4981 agcagattgg acactacaga cagaagctcc agctcattga acggttttgc gagataattg
     5041 tctgcgcctg ccgtgtgagc ttcctgcctg gttcgcgtat ccgctttcgc ggtgagcaat
     5101 attaccggta gagtttttag ttcagggtcc gcccgcacgt ttgcaatcag gtcgattccg
     5161 gacatgcgcg gcatcatgag atctgtgatg atgagggaag ggcgtctgga tcttactgca
     5221 gtccatccgt gctggccatc cgttgtttct actactgtgt atccgcttct tcgtagaatt
     5281 ccgctgatgt atccacgcat tcctctattg tcttctacaa gaaggatggt agttcccgaa
     5341 gcctgtacat gtgcgctcgc atcatccgct gccacctcat ctgcaagttc aatgtccgcc
     5401 atgacggaaa cgcttgaatc gtgttccggt agaatttcgg aaatctcagg aagccagaaa
     5461 tagaaagagg ctccgttgcc gggaacactc ttagctgata catggccgcc gtgcagcgcg
     5521 atcagttctc ttaccaggga aaggccaagt ccagttcctt cttgatcgcg cgtaagcgac
     5581 ggttcacttc ttccgaatcg tgtaaagagc ccggcctggt tttcgtctga aattccagct
     5641 ccactatcat gcactgccac ttccacacca ttgtcttttc tatgcgcgca aatgcgaacc
     5701 ctaccgccgg cgggtgtgaa cttcacagca ttcgacagat agttgtacac gcacttttcg
     5761 atcatgtccg ggtctgctgc cactcgtgga atgtccccat ctatttgcgt ttcgatctgg
     5821 attcgtttcc cgtttgcata aggctggaat gcttcgctga tggatccgag catttcgccc
     5881 aggttcatgg atcgtagtcg cagttccatt ttacccgcgg cgattttctg caaatccagg
     5941 agttgattta cgagacgaag aagtcttcgc gcattggaaa gaactgtgcc cagctcttct
     6001 gttgcgattc cctcttttct tctgactgca tccacgaccg gaccgtaaat caaagtgaga
     6061 ggagtgcgga gctcgtgcga tatgttctgg aaaaaagcag tcttttcctt gtcagcctga
     6121 agtagttttt cggacgcctg tctgagttca tccgtttgct gtcgcacttc ttcgcgcaaa
     6181 ttttgggaca gatgttccgc agttgcaaat gcttttgcgg aaacgcgtgc aaggacagtg
     6241 gcctgagaaa aaatcaaaac aaaaaatcca accggggtga ggaaatctcc gggaatgacg
     6301 ccggcggcca caagtatgtc attcatcccg gctgcaccgg caatgcttgc accgccgaga
     6361 actgtgatcg ctccatctct cttgcgaatg gcggccagag caatgccgta gatcatgtaa
     6421 acgatcatga ttgcagcggc caat
//
LOCUS       DAORFF010000173         6378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6383_length_6378_cov_3.133955, whole genome shotgun sequence.
ACCESSION   DAORFF010000173 DAORFF010000000
VERSION     DAORFF010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6383_length_6378_cov_3.133955"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PKK76_08485"
     CDS             complement(<1..288)
                     /locus_tag="PKK76_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNL68550.1"
                     /translation="MTDAEFQAMLEEYSFDLPEDLIARHPPRERDAARLLLVDPVRGE
                     FREESFRNLPNLLQPRDCLVLNRSKVSFRRLHVQRRDRTFEPLFCSMKEGAW"
     gene            369..1859
                     /locus_tag="PKK76_08490"
     CDS             369..1859
                     /locus_tag="PKK76_08490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNL68551.1"
                     /translation="MGTWKRTLQRLGDLFSTSRAVRIGCLCLLLLAVAVTSLRIIKDP
                     SSSGLIASSRSDFDDYYTASGMVGRGEDPYRVEQLQGIMELPRSFRPEDLLNPAKLIE
                     LMSRFKGLGTYLYLPFTAFVLLPLSGLDYKVAAGIFQFLSIFAFVAFIAFLHRSLRRD
                     RPLITEREFTLPLLAAFSLLGGFLSENAANGNIAFFLLLLTGPGIYYSFSDKWWAQVA
                     GGMLIGTAAVMKVTPVFLVLVLAASIRVYALVGVGLGIGLGIFLPALGLGWDRNLALL
                     QGWYNLILETYNKYVFLRPWANNQTISGMIGKLFLPGSDLKQSEYGLPLIGGLPDKQM
                     YASLGLLVKGINAFLLGITGIASVVSAIRLFKNGFQSLVRSVFGSFDARPAFFEDVSM
                     VRLLWLVTLVSLVTAGVSWYHAYCVLLLPWFLRFVQVFSGEKLLLGEKVAIAFTAFFS
                     LGPLMMGSHLREGLALYSIFTFGVCFQALYLAWLVIASNRRPADGI"
     gene            1849..3357
                     /locus_tag="PKK76_08495"
     CDS             1849..3357
                     /locus_tag="PKK76_08495"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNL68552.1"
                     /translation="MVFNSGVYIYFLIAVFTIYWAVSRLPRGAHRAQNTVLLLSSYFF
                     YGWWDWLYLVLIFASTTLDYFVGIGLDSLTSERKRKLLITFSISCNLTILGIFKYYDF
                     FITSFISMFRQFDPGAFPDGGAHLLLNAILPVGISFYTFQSMAYTVDVYRRQVRAERN
                     FLDFASFVCFFPQLVAGPIERAGDLLVQMKNNRQFTWENMRNGAWMILLGFYMKTYVA
                     DNISPLVNLVYLDHKFAYHQNPLAASGHGGLQGILASVGFAFQIYSDFWGYSAIAMGS
                     ALFLGFKLTQNFNAPQMAENPADLWRRWHITLNRWVTDYIYIPLGGSRLGVVRKYVNL
                     FVAMLLMGLWHGASWTFFVWGGFMGAWLIAHQIFRDYLPKLSEGAPAWKKGTVKALKM
                     AATFGVFGLSATCFRAYDIHQTGELWKSILDISSYSLSPVHGVMGAGDFLWALVKKLI
                     VLLVIDFMAYRTGSLYWIFDRPVWVRITLYSVLFFFVLILGEFGKDVIYFAF"
     gene            3335..4585
                     /locus_tag="PKK76_08500"
     CDS             3335..4585
                     /locus_tag="PKK76_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68553.1"
                     /translation="MSSILRFKYPALLGAVAVIAILEIAARIIPLEYTSGAGIFLTEK
                     RRALIEASSPEYDYIILGDSRSLSLNGHPRTDKEPYTVYNLSIPAMGPRYFQYYLEKY
                     LHNRKHKPAAVVFAGDPGLFQRSWYTPNHDSKMIYADSVEEGLGHYLWSRFHKRIQYL
                     KAGKYPDQRDQFGAMVWEVFSHRYLHLFSVSDMMEQYTGAERIFMLRESIPNAFYLYR
                     YRDGIRQYTFDLRANSFRKHELPDFCNACSGLVRAECHPDYSRVEDNQKLDALIEKNY
                     GGVNLGDRLDPAQRFNYYQMRDTAVEHQVDWFSREEPEMKPVEDLIRTAAKHDIKIVF
                     SDVPAVEPMRTVRYHKIYFERLAQIVSKYPGARIVRFPDPYYPVKLFIEQVHYECEGA
                     ARLNQEFYSSVMPEVVKFAPRSAP"
     gene            4582..5670
                     /locus_tag="PKK76_08505"
     CDS             4582..5670
                     /locus_tag="PKK76_08505"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 domain-containing protein"
                     /protein_id="HNL68554.1"
                     /translation="MKSNLRVVYFPLILFLVYFGLDKTCLLPQMKKLSQPDATYLYFD
                     YKTDLIDQVEKIHKANQKPENFVSGVRKKTLIILGSSRLLYFDTARFHRNYPDWELFN
                     FSAPVTSPAYYAYIFERILERGVKPDYVVVEVDPLQYNDGSDHFVRSNLAYSFDLPFI
                     LKHHDLFKNSEISFFLARFLFAGYKYPPDLQAIASRARDPNDRWLLALNELDKYQREN
                     RGAGRSIIPRENWYERDFARLELSSRHTISWLYGNFELSERQFGFLEMLLRDARSAQV
                     PVLLLRPQVSSTMQRQLDEDSRLAAPLAEWEVRLKKEQKDFSVPYLDLRHDRDVSCNT
                     FVDGAHMALDCYHMLTVAVMREYRANGL"
     gene            complement(5657..6133)
                     /locus_tag="PKK76_08510"
     CDS             complement(5657..6133)
                     /locus_tag="PKK76_08510"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNL68555.1"
                     /translation="MTARQSVIAFYVALAAIVAGGGMLLFYNFHTLSRNAEPVIAVTR
                     SGLRFYREESAARSEAEKTGKPLFIEFGALWCEPCLQFNEKMDKDPALQTALSGAVLV
                     RIMDTDPTFESYSSRKGYEALADGLPFFVIESRGEIKLASHEWKNTAAIARAIKGR"
     gene            complement(6130..>6378)
                     /locus_tag="PKK76_08515"
     CDS             complement(6130..>6378)
                     /locus_tag="PKK76_08515"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNL68556.1"
                     /translation="DWKEAIAKKFIQATPRSVAIPDGVQDEQMGARVLRGKGDFVYEA
                     RFDKLEGQTIKVRSQICDDVKGICYRPTTQDIPVVSVK"
BASE COUNT         1387 a         1528 c         1624 g         1839 t
ORIGIN      
        1 ccaggcgcct tccttcatgg aacagaacaa aggctcgaag gtccggtccc gtctttgtac
       61 gtggagcctg cgaaatgaaa ctttgctgcg gttgagaacc agacaatctc gaggttgaag
      121 aaggttcggc aggttgcgaa atgattcctc gcgaaattcg ccccgcactg gatcgacgag
      181 caggagcctc gctgcgtctc tctccctggg aggatgcctg gcgatcaggt cttcgggcag
      241 atcaaaggag tattcctcaa gcatagcctg aaattcagcg tctgtcaccg ggctagagtt
      301 cgaggaagac cttctgtgcc caggagaatt ttcatttcaa aagctcgtat gatcaggaaa
      361 attggacgat gggcacctgg aagcgcaccc tgcaaagact gggcgatctt ttctccactt
      421 ctcgcgcggt caggatcggc tgcctgtgcc ttttgctact ggcagttgcc gtgacttctc
      481 ttagaatcat caaagatcct tcttcgtctg gtctgatcgc ttccagtaga agcgatttcg
      541 acgattacta caccgcgtct ggcatggtcg gaagagggga agacccttat cgcgtggagc
      601 aattgcaggg gatcatggag ctacccagga gctttcgccc cgaggatctt ctcaatcctg
      661 cgaagctcat cgagctcatg agtcgcttta aggggctggg cacctatctg tatttgccct
      721 tcacagcgtt tgttcttctt cctttgtctg gcctggatta caaagtagct gctggcattt
      781 ttcaatttct gtctattttt gcgttcgttg cctttattgc atttttacat cgttctctgc
      841 ggcgagacag gcctctcatc acagaaagag agtttactct cccacttctt gccgccttct
      901 cattgttagg tggtttcctc agcgaaaacg cggccaatgg caacatagcg tttttcctat
      961 tgcttcttac ggggcccggg atctactatt ccttttcgga caaatggtgg gcccaggtgg
     1021 caggtggcat gctcattggc acggcggcag tgatgaaggt gactccggtg tttctggttc
     1081 tggtgcttgc cgcttcgatt cgagtgtatg cactcgtggg agtgggcctt gggattggcc
     1141 tcgggatctt tcttcctgct cttggtctgg ggtgggatcg caatctggct ttgttgcagg
     1201 gttggtacaa cctgattctt gaaacctata ataagtatgt gttcctcaga ccctgggcaa
     1261 acaaccagac gatttctgga atgatcggta agttattttt gccaggcagt gatctcaaac
     1321 aaagcgagta tggtcttcca ctgatcggag ggctgccgga caagcaaatg tatgcttctc
     1381 tggggctgct cgtcaaaggc atcaacgctt tccttctggg aattacgggc attgccagcg
     1441 ttgtcagtgc gattcgcctc tttaagaatg gctttcaaag tctggttcga tccgtgttcg
     1501 gcagtttcga tgcgcgccct gctttttttg aagatgtttc tatggtgaga ctcttgtggc
     1561 ttgtgaccct cgtttctctc gtgactgcag gagtgtcctg gtatcatgcg tactgtgttc
     1621 ttctacttcc ctggtttctt agatttgtcc aggtgttttc cggcgagaag cttctgctcg
     1681 gggaaaaggt agcgattgcg tttactgcat tcttttcgct tggaccactg atgatgggtt
     1741 cgcatttgcg cgaaggtctg gcgctctatt ccatttttac attcggcgtt tgttttcagg
     1801 cgctctatct ggcatggctc gtaattgcgt cgaacaggag accggccgat ggtatttaat
     1861 tccggcgtct atatttactt tctcatcgca gtattcacca tctactgggc cgtttcgcga
     1921 ctgccaaggg gcgcgcatcg cgcacagaat actgttcttc ttttgtcttc gtattttttt
     1981 tacggatggt gggactggct ctatcttgtt ttgatttttg cgtccacaac tctggattat
     2041 tttgttggga ttggcctgga tagccttacg tctgagagaa agcgaaaact gctgatcacc
     2101 ttctccatct cgtgcaatct tacaatactt gggattttta aatattacga tttctttatc
     2161 accagtttca tttcgatgtt caggcagttc gatccaggcg cattcccgga tggaggagcc
     2221 cacctcttgt tgaatgcgat tttgcccgta gggattagct tctatacttt ccagagcatg
     2281 gcttatactg tggatgtgta cagaagacag gttcgcgcgg agcgcaactt ccttgatttc
     2341 gcttcttttg tttgtttttt cccacaactt gtagcgggtc ccattgagag agccggcgat
     2401 ctgctggttc agatgaagaa caatcgtcag tttacctggg aaaacatgag aaacggtgca
     2461 tggatgattt tgctcggttt ctacatgaag acctacgttg cagataatat ttctccgctc
     2521 gtcaacctgg tgtatctgga tcacaaattc gcatatcacc agaatccact cgctgcaagc
     2581 gggcacggcg ggttgcaggg aattcttgca agtgtagggt ttgcgtttca gatctattct
     2641 gacttctggg gatactccgc gatcgccatg ggctccgctc tatttctggg attcaagctc
     2701 actcagaact tcaatgcgcc gcaaatggcg gagaatccag cggatctctg gaggcgctgg
     2761 cacatcactc tcaatcgctg ggtcaccgac tacatttaca ttcctcttgg aggaagcagg
     2821 ctcggagttg ttcgcaaata tgtgaatctc tttgtcgcca tgttgctgat gggcctgtgg
     2881 cacggagcaa gctggacatt ttttgtctgg ggtggtttca tgggagcgtg gctgatcgca
     2941 caccagatct tccgcgatta tcttccaaag ctttctgaag gggcgcctgc ctggaagaag
     3001 gggacagtga aggctttgaa aatggcggcc acgttcggtg tgttcgggct ttctgccact
     3061 tgctttcgcg cctatgacat tcatcaaaca ggtgagctct ggaaatcgat tctggatatt
     3121 tccagctact cgctttcgcc tgttcatgga gtgatgggtg cgggcgattt cctctgggca
     3181 ctggtcaaga aactgatcgt cttgcttgtg attgacttca tggcctatcg cactggatct
     3241 ctttactgga tttttgatcg tccggtgtgg gttcgcatca ctctgtattc tgtgcttttc
     3301 tttttcgttc tgatcctcgg ggagtttggc aaagatgtca tctattttgc gttttaagta
     3361 tccggcactc ctgggcgccg tggcggtcat tgcgattctc gagatcgcag ctcgaatcat
     3421 tcctcttgag tacacttcgg gcgctggaat ctttctgact gaaaagagac gcgccctcat
     3481 agaggcttct tctcctgagt atgactatat aattctgggc gattcgcgca gtctcagtct
     3541 gaacggccat ccgcgaacag acaaggaacc ctacacagtt tataatctga gcataccggc
     3601 catgggtccg aggtactttc agtactactt agaaaaatac ttacacaatc gtaagcataa
     3661 accagcagcc gtcgtttttg ccggagatcc tggtcttttt cagagaagct ggtatacgcc
     3721 caatcatgat tcgaagatga tttacgcgga ttctgtagag gagggtctgg ggcattacct
     3781 ctggtccagg tttcacaaga gaattcaata cctgaaagcg ggcaaatatc ccgaccagag
     3841 ggatcagttt ggtgcgatgg tatgggaagt cttctcacac agatatttgc acttatttag
     3901 tgtttcagac atgatggaac agtacactgg tgcagagcga atcttcatgc ttcgtgaaag
     3961 catccccaac gctttttacc tctatagata cagagatgga atcagacagt acacgtttga
     4021 cctcagggcc aattcgtttc gcaaacacga actgcctgat ttctgcaatg cttgttctgg
     4081 cctggttcgc gctgaatgtc atccggatta ttccagggta gaggataatc agaaactgga
     4141 cgcactgatt gagaaaaact atggtggagt caacctgggg gacagactcg atccggcaca
     4201 aagattcaac tactatcaaa tgcgagacac tgcagtcgag catcaggtgg actggttttc
     4261 gcgcgaagag cctgaaatga agccagtgga agatttgatc cgcacagctg caaagcatga
     4321 tatcaagatt gttttctcag atgtgccggc cgttgaacct atgcgaaccg ttcgatatca
     4381 caaaatttat tttgaaagac tcgctcagat tgtaagcaag tacccgggtg cacgaattgt
     4441 acgcttcccg gatccgtact atccggtgaa actgttcata gagcaggttc actatgaatg
     4501 cgaaggtgca gccaggctca accaggagtt ttactctagc gtgatgcctg aagtggtaaa
     4561 gtttgcgccg cggagcgctc catgaaatcc aaccttcgcg ttgtttattt ccccttaatc
     4621 ctgtttctcg tttactttgg tctggacaag acctgccttc ttcctcaaat gaagaagcta
     4681 tctcagccag acgcaaccta tctatacttc gattataaga ctgatttgat cgatcaggta
     4741 gagaagattc acaaagcaaa tcaaaagcct gaaaacttcg tttccggagt caggaagaaa
     4801 accctcatca tcctcggcag ttccaggcta ttgtactttg atactgcgcg ttttcatcgc
     4861 aactatcctg actgggaatt gttcaatttc agcgcgcctg taacctctcc ggcgtattat
     4921 gcgtacatat tcgaacgcat tctagagaga ggagtgaagc ctgattatgt ggtcgtggaa
     4981 gtagatccgc ttcaatacaa tgatggatcc gatcattttg taagatccaa cctggcgtac
     5041 agttttgatt tgcctttcat tttgaagcat cacgatcttt ttaagaattc ggaaatttca
     5101 ttcttcctgg ctcgattctt gttcgcgggt tacaaatatc ctccggatct tcaggcaatc
     5161 gcttcgcgcg ctcgcgaccc gaacgatcgc tggcttcttg ccttgaatga attggacaag
     5221 taccagagag agaatcgcgg agcaggcaga tcgattattc cgcgcgaaaa ctggtatgag
     5281 agagacttcg cgaggcttga gctttcttcg cgtcacacca tttcctggtt gtatggaaat
     5341 tttgaattgt ctgaaaggca attcgggttt ctggagatgc ttctgcgaga tgcccgctca
     5401 gcccaggttc ctgttctgtt gcttcgtccc caggtgtctt ccacgatgca aagacagcta
     5461 gacgaagact ctcgtctggc agcacctctg gctgagtggg aagtgcggct gaagaaagaa
     5521 cagaaggatt tttctgtgcc ttatctggat ttgcggcatg accgcgatgt gtcgtgtaac
     5581 accttcgtag acggagcgca catggctctg gattgctatc acatgctcac agtggctgtg
     5641 atgcgagagt atcgcgctaa cggcctttga tcgcccgggc aatggctgct gtatttttcc
     5701 attcgtgact ggccagtttg atttctccgc gtgattcaat gacaaagaat ggaagtccat
     5761 ctgccagtgc ttcatatccc tttcttgagg agtaagactc aaaggtagga tccgtatcca
     5821 tgattctcac aagcaccgct ccagagagcg cggtttgcag ggccgggtct ttgtccatct
     5881 tctcattaaa ctggagacag ggttcacacc acagtgctcc gaattcaatg aagagtggct
     5941 tgcctgtctt ctctgcttcg cttcgcgcgg cggattcttc tctatagaat cgcagtccac
     6001 tcctggtcac ggcgatcact ggctcagcat tccgggatag cgtgtgaaag ttgtaaaaca
     6061 gtaacattcc gccaccggcc acgatggccg caagcgccac atagaacgca atgacggatt
     6121 ggcgagcagt catttcacgg aaacgactgg aatatcctga gtggtgggtc tatagcagat
     6181 tccttttacg tcatcgcaga tttgagagcg cactttgatg gtctggcctt cgagtttatc
     6241 aaatctcgcc tcgtatacaa agtcgccttt gccgcgaagg acgcgtgcgc ccatttgttc
     6301 gtcctggact ccatctggga tggctacaga tcgcggagtt gcctggatga attttttagc
     6361 aatcgcttct ttccaatc
//
LOCUS       DAORFF010000174         6350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6426_length_6350_cov_4.142971, whole genome shotgun sequence.
ACCESSION   DAORFF010000174 DAORFF010000000
VERSION     DAORFF010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6426_length_6350_cov_4.142971"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..3094)
                     /locus_tag="PKK76_08520"
     CDS             complement(119..3094)
                     /locus_tag="PKK76_08520"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNL68557.1"
                     /translation="MNPEEISARIQRIENDLAELKAAVRQQSSSSSTQTAETRPTSSV
                     TTPPRPPAKPGIVTETLSRFKEDNVEVFLGGNLLGKLGIVAIVLAVSWFIKLAFDNQW
                     VNESGRIYTGLLVGFAIAGVAVSLAKRNMRVVPAPLFGAGISIVYISLFSAYHFYYLL
                     GIQEVFVSLFVLSVAATVLSLTANRQSLYVFGLIGSFLAPIMLSRGENSYRFLFTYIA
                     VIQLGFLWVSWYRPWRISAVIVLAGTAIMWTGWASNYLNKSGFVFPMAILVFTLAAFF
                     VRHTFLSLRHKTAPSIPDLIILPLSSLFYASAGYYLTRHHYPNAISHFLLVLTLLMAF
                     FYIRYTAIEARDNSTRSILFFQILVFTFASISQFFDGRLLTLSWIVFASALSISSVTL
                     KTRPVLLVSMIAWILAVFRLIFAEYTPSPFHPLVNVRFALYLVAGVGASITYLRHRKD
                     PFETLVRGFAFLSMFLFVFGTLLENRDFIASPHYRNLGYSYVLGLYAAIFLVYGFLRN
                     HKTMRLCGVFLAALVVMKLYFYDIWTMSRLVRIIAGFSLGVALVVLSIVYQKYRDKIL
                     QIGKTLMMWLAAPLALFILLSSNNLQAETIRAGSFKKVASLKPDNPSALKDSKTAYGR
                     FTMNDDLVRAGSSSVRIAWKDRALPYVLQRVREDKQRTGAAVPEVIFEEGQRNARVFV
                     LRLPEAPGGTVWTDLEVESTEMYESGVQVEAGEKPGDWNVIGHRNIYNYAGASDRVIS
                     IDAGRSKYLRVTLDKNLHMVFPKVHYGPSRDREVSFDIPPEEWKITQNTDEHATEFLF
                     NNKDQRKIERLTLQFAEPRFHRRVEVYVMQRASHEFVINTTTGFMKRTNDPADQVLDL
                     ASPATDAVKVVVFNEDDAALKLTKSTAFAPLEQVIFQLPSADELATEEGNPEIKIYAG
                     NEYVGFPDYDLSRTMDDSVVSASFTPTALGDNAAFAYSAVEPPLSSWIIRIAFYIGLA
                     ALAIPGLRVFRDYAQSVKS"
     gene            complement(3091..5592)
                     /locus_tag="PKK76_08525"
     CDS             complement(3091..5592)
                     /locus_tag="PKK76_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68558.1"
                     /translation="MKSSRRKNSKRASHPERITSLGVPLVQGERGRAQLDLTDLKLFR
                     GLSVLAGMLGEEILDQARRGTDISVLYRILPEITPELAELGIAGVSIHARKNVLKGLP
                     QYRKEFHTQIRAVFGTLQRPAWGGILFPEYFGLTQKKKEPPGLLFPYHLHFEQEAIDY
                     FFLVERDIEGKFLRITIETEKEGRLDLKRISHVPVNDLDRRVYLQGLTRMAESMHQGI
                     RRECENFQNEHNEDRGRQPNFFEQINSAGMEHCEIITIHWPVESEEYLVSSAPEQTVD
                     FLRKCLIVLEDREIGRRLSQGEIEVISGPRRAYLDLSRRGRCLNISLDLPRVFPDIRR
                     YLTRMPQLEKLSLKKPDAMKGVRVLLIHHITGEILATIRAIENMNAEFLRVLFVKYAG
                     IVPPEYLETLLSLPENQFAFHGLNKIEASGSVEGFYILSRQYSPITRFQDLDRRLAEK
                     KLAFFEAMQLAAGNLFFREAGICIRTNKKLLLVEDGGYLAPQLNALSLEKKSVRDAMI
                     YYGFDPGKKNEGLKEILPVSDKDLEKPLGEWLSAFFTGSVEHTRNGYDRLVGVQKKYN
                     RLQFPALTIAVSKTKREMESREVSVSILHAVESILHGQGLVLSNRRALVLGSRGAIGQ
                     CLVQHLKSRIQPDRVAGVDLVINSKNSSAQGITEAAALEKLGKEHVRDADIILGVIGK
                     SIFTDEWIEDLILNGKRPHVFFASGSTKTLEFTHLSHYVEKLRQAQSPRIRGQAARVD
                     AEPIRDPQTGLIQGTQVRIELDKKTRTLHLLGGLTPINFLYYGVPTETMDPVLTQLIQ
                     SAAGLLARQKSGKGLPPRVLAVDHEITVDAELIKK"
     gene            complement(5546..5884)
                     /locus_tag="PKK76_08530"
     CDS             complement(5546..5884)
                     /locus_tag="PKK76_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL68559.1"
                     /translation="MAQFDVKKLANADCVGISGRFTLEDKDAFLDIMTGKLDIQNRQA
                     IMECSRMEYIDSSGIGDLWKLRTEATRFGKTVILASLKESVRKTFRTAQLESVFEILS
                     EEEFKARFPS"
     gene            complement(5871..>6350)
                     /locus_tag="PKK76_08535"
     CDS             complement(5871..>6350)
                     /locus_tag="PKK76_08535"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNL68560.1"
                     /translation="GLHPTTRMCIRWFCEQGVRDLRIIDAGCGSGILSLAALLLGARA
                     VLAFDIESNAVRATQQNLALNPGIDSTRLSILQGGFELAELSSFNAEIFVGNLTANII
                     LGAKDRIQSCRFPRMVMTGILSEQKNEIISAFENHRLKSELEDDGWCLLELVKDGSI"
BASE COUNT         1559 a         1537 c         1638 g         1616 t
ORIGIN      
        1 tttgcaatcc cctcgcgagc atgtttctgt ctgggcgaga agttggcctc ccatggaaag
       61 tgtgacgatc agaaccaaag tggcagattt catggcgatc ctcaaaagtt taagttcttt
      121 atgattttac tgattgagca tagtcgcgga acactctcaa gcctggaatg gcgagtgctg
      181 ccagaccaat gtagaatgcg attcggatga tccagctgga aagcggtggc tccactgctg
      241 agtatgcgaa cgctgcgttg tcaccgagag ctgtcggcgt gaaacttgcg gacacaactg
      301 aatcgtccat tgtgcgtgag agatcgtagt ccgggaatcc aacgtattca tttcctgcat
      361 agatcttgat ttctggattc ccctcttccg ttgccagttc atctgcagaa ggaagctgga
      421 atatcacctg ttctagcgga gcgaacgctg tggattttgt tagcttaagt gctgcgtcgt
      481 cttcgttgaa tacaacaacc ttcactgcat ctgttgcggg acttgccaga tcgagtacct
      541 ggtctgcggg atcgttggtc cttttcatga atccagtggt cgtattgatc acgaattcat
      601 gtgaagcccg ctgcatcacg tacacttcca ctctcctgtg aaagcgtggt tcagcaaact
      661 gaagcgtcag tctctcaatc ttgcgctggt ccttgttgtt aaagagaaat tcggtagcgt
      721 gttcgtctgt gttctgcgtg attttccatt cttctggtgg tatatcaaaa gagacttcgc
      781 gatcgcgcga agggccgtag tgcacttttg ggaaaaccat atgaaggttc ttgtcgagtg
      841 tcactcgcaa atatttggag cggcctgcat caatgctaat gactctgtcc gacgcgcccg
      901 cgtagttgta gatgtttctg tgaccgatca cgttccagtc tcctggtttt tctcccgctt
      961 ctacctggac gcccgactca tacatttcgg ttgattctac ttccagatcc gtccagacgg
     1021 ttcctcccgg tgcttcgggc aatcggagga caaagactcg agcgtttctc tgtccttctt
     1081 caaagattac ttcgggtact gccgcgccgg tgcgttgttt gtcttcgcgc actctctgta
     1141 acacgtacgg gagcgcgcga tctttccagg caatgcggac ggaggaggat cctgcgcgaa
     1201 ccagatcgtc gttcatcgtg aatcttccgt aggccgtttt tgaatccttt agcgcagatg
     1261 gattgtccgg ctttaaagag gcaactttct tgaagctacc agcacggatc gtttccgcct
     1321 gcaggttgtt agacgaaagt agtatgaata gagcaagagg agcagccaac cacatcataa
     1381 gagtcttccc gatttgtaga attttgtcgc gatacttttg gtacacgatg ctgagtacaa
     1441 caagtgcaac gcccaggctg aaccctgcga tgatgcgaac aaggcgactc atggtccaga
     1501 tgtcatagaa gtagagcttc attacaacta gagcggcaag aaacacgcca cacagtcgca
     1561 tggttttgtg atttcgcagg aaaccgtaga cgaggaagat cgcggcatat agtcccagaa
     1621 catacgaata ccccaggttg cgatagtgcg gcgacgcgat gaaatccctg ttttctagca
     1681 aagttccaaa aacgaacaag aacatggaga gaaatgcaaa tccgcgcacg agggtttcaa
     1741 acggatcttt tctgtgcctg agatacgtga tggaggcgcc tacgcctgcg accaggtaga
     1801 gagcgaatcg cacattgacc agcggatgaa acggcgaggg agtgtattct gcaaagatga
     1861 ggcggaatac agccagaatc caggcaatca tgcttaccag gagaacgggt ctggttttca
     1921 gagtgaccga agaaatggaa agagcggaag caaatacgat ccaggaaagg gtcagaagcc
     1981 tgccatcaaa gaattgcgaa atggatgcaa acgtgaatac gagtatttgg aagaagagaa
     2041 tactgcgagt gctgttgtct ctggcttcaa ttgctgtgta gcgaatgtag aagaatgcca
     2101 tgagcagagt gagaaccaga agaaagtggc tgatcgcatt cgggtaatga tgtcgcgtca
     2161 gatagtaacc agcggaggca taaaacaaag aggatagcgg cagaatgatc aaatctggaa
     2221 tggatggagc tgtcttgtgt cgcagagata gaaacgtatg tcgaacgaag aaagcggcaa
     2281 gagtgaatac gagtatggcc attggaaaaa caaagccact cttgtttaga taattagagg
     2341 cccatccagt ccacatgatc gcagttcctg ccagcacgat gactgcagaa atgcgccagg
     2401 gtctgtacca gctcacccac agaaaaccaa gttgaatgac tgcgatgtat gtaaagagaa
     2461 agcggtagga gttttccccg cgcgaaagca tgatgggtgc cagaaatgaa ccgatcaacc
     2521 cgaagacgta gagactctgt ctatttgcag tgagagaaag cacagttgcg gcgacgctca
     2581 gcacaaacag acttacgaaa acttcctgaa tgccaagcag gtagtagaaa tgatacgcgc
     2641 tgaatagcga aatgtagaca atggaaatgc cagccccaaa cagaggagca ggtaccaccc
     2701 tcatgtttct tttggccaga gacactgcaa cacctgcaat tgcgaaacct acgagtaagc
     2761 cggtgtaaat tctacccgac tcattgaccc actggttgtc gaatgcaagt ttgatgaacc
     2821 acgaaacggc cagaacaata gcaacaattc cgagtttgcc aagcaggttt ccgccaagga
     2881 atacttcgac gttatcttcc ttgaagcgtg atagagtttc tgtaacaatt cctggctttg
     2941 cgggcgggcg cgggggagtc gttacgctag aagttggccg ggtttctgcg gtctgtgtag
     3001 atgaggatga cgattgttgt ctgactgctg ctttgagttc tgcgagatca ttttcgattc
     3061 tttgaatgcg cgcgcttatt tcttctggat tcatttcttg atcagctccg catccactgt
     3121 aatttcatga tccaccgcaa gtacgcgcgg cggtaaacct ttgccggact tctgtcgtgc
     3181 cagcaagcct gccgcagact ggatcagctg cgttagaact ggatccatgg tctctgtcgg
     3241 caccccatag tacaaaaagt taatgggtgt tagaccaccg agtagatgga gggttcgagt
     3301 tttcttatcc agttctatgc gaacctgcgt gccctgaatg agccctgtct gggggtctcg
     3361 tattggttct gcgtctactc tggctgcctg tccacgaatt cgcggagatt gagcttgtct
     3421 gagtttttca acgtaatgcg acaaatgcgt gaattccaga gtcttcgtgg acccgcttgc
     3481 aaaaaacaca tggggacgct ttccattcag gatgaggtct tcgatccact catccgtgaa
     3541 gatggatttt ccaatcacac ccagaatgat gtccgcgtct cgcacgtgtt cctttccaag
     3601 tttctcaagt gcggccgctt ccgtgattcc ttgagcggat gaattcttgc tattgattac
     3661 cagatcgact ccggcgacgc ggtccggttg aatgcgcgat ttcaaatgtt gcacgagaca
     3721 ctgaccaatg gcccctctgc ttccaagcac gagagcccgg cgattagaaa ggacaagccc
     3781 ctgtccgtgt aaaatgcttt ccactgcgtg caggatagaa accgaaactt cgcgcgattc
     3841 catttctcgt ttcgtttttg aaactgcaat ggtcagtgct ggaaactgaa ggcgattgta
     3901 tttcttctga actccaacca gtcggtcgta tccattgcgt gtgtgctcta cgctgccggt
     3961 aaagaaagcg ctgagccatt ctcccagggg tttttccagg tctttgtcag agacaggcag
     4021 gatttctttt agaccttcat tcttctttcc aggatcaaat ccgtagtaga tcattgcgtc
     4081 gcgtacggat tttttttcca gagatagagc attcaattgc ggcgccaggt aaccgccgtc
     4141 ttcaacgagc agaagcttct tattggttcg aatgcaaata ccggcttcgc gaaaaaacag
     4201 attgcccgct gccagttgca tcgcctcaaa gaaagcgagc ttcttttccg ccagtctgcg
     4261 atccagatcc tggaatcgcg taatgggcga atattgtctg gatagaatgt aaaacccttc
     4321 cacgctcccg gaagcttcaa ttttgttgag cccatggaag gcgaactggt tttcaggaag
     4381 agatagtaaa gtttctaaat attccggtgg aacaataccc gcgtacttaa caaagagtac
     4441 gcgcagaaac tccgcgttca tgttttcgat cgcgcgaatt gtggccagga tttctccggt
     4501 gatatgatga atcaagagta cgcgaacgcc tttcattgcg tctggtttct tcaggctgag
     4561 cttttccagc tgaggcatcc gtgtgagata gcggcgaatg tcggggaata cgcgcggaag
     4621 atcgaggcta atgttgaggc atctgcctct tcttgaaagg tcgagatagg cgcgtctcgg
     4681 gcccgaaatg acctcaattt cgccttgaga aagccgcctt ccgatttctc gatcttccag
     4741 cacaatcaaa cattttcgca ggaaatcaac tgtctgctca ggcgcggatg aaaccaggta
     4801 ttcttcgctt tcaacgggcc agtggatcgt tatgatttcg cagtgctcca tcccggccga
     4861 gttgatctgc tcaaagaaat tgggctgacg tccgcgatct tcgttgtgct cgttctggaa
     4921 gttttcgcat tctctgcgga tgccctgatg catggactcg gccatgcgcg tcaatccctg
     4981 caaataaacc ctgcgatcga gatcgtttac aggcacgtgc gaaattcgtt tgagatcgag
     5041 ccttccttct ttttctgttt ctatggtgat gcggagaaac tttccctcga tgtctctttc
     5101 tactaaaaag aaataatcaa tcgcttcttg ttcaaaatga agatggtacg gaaacaaaag
     5161 tccaggaggt tctttcttct tctgcgtcag gccaaagtat tcgggaaaaa gaattcctcc
     5221 ccaggccggg cgctgcagag ttccgaacac ggcgcgaatc tgagtgtgaa attcttttct
     5281 gtattgtgga aggcctttga gtacgttttt ccgcgcatga atggacacgc ctgctattcc
     5341 aagctccgcc agttccggtg tgatttcggg aagaatcctg tagagaacag aaatatccgt
     5401 tccgcgtctt gcctgatcga ggatctcctc tccgagcatt cccgcaagca ccgacagtcc
     5461 ccgaaacagt ttcaggtctg tcaggtcgag ttgtgcgcgt ccgcgctccc cctgaacaag
     5521 cggtacacct agagaggtga ttctttcagg atgggaagcg cgctttgaat tcttcctccg
     5581 agaggatttc aaatacggac tccagttggg cggtgcggaa agtcttgcgt acgctctctt
     5641 tgagagacgc gaggatgact gttttaccaa atctcgtggc ttccgtgcgg agcttccaca
     5701 aatctccgat tccggaagaa tcaatatact ccatgcgcga gcattccatg attgcctggc
     5761 gattctggat gtccagcttc ccggtcatga tgtccaggaa ggcatctttg tcttcgaggg
     5821 tgaacctgcc agagataccg acgcaatctg catttgccag tttctttaca tcaaattgag
     5881 ccatctttca ccaactccaa caaacaccat ccatcatctt ccaactcgct tttcaggcgg
     5941 tggttttcga atgcagatat gatttcgttt ttctgttctg atagaatgcc tgtcatcacc
     6001 atgcgtggaa accggcacga ctggattcta tctttcgcac cgaggatgat atttgcggta
     6061 agattgccca caaatatttc agcgttaaat gaggaaagct ccgcaagttc gaagccaccc
     6121 tggagaatgg agaggcgggt ggaatcgatg cccgggttca gggccagatt ctgttgtgtg
     6181 gcgcgaactg cattggactc aatgtcgaag gcgagaacgg ccctggcacc aagcagcaaa
     6241 gcggccaggc tgagaatgcc ggacccacag ccagcatcga tgatgcgaag atcgcgcaca
     6301 ccctgttcac agaaccagcg aatgcacatc ctggtggtag ggtgcagacc
//
LOCUS       DAORFF010000175         6320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6458_length_6320_cov_3.014525, whole genome shotgun sequence.
ACCESSION   DAORFF010000175 DAORFF010000000
VERSION     DAORFF010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6458_length_6320_cov_3.014525"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="PKK76_08540"
     CDS             <1..1302
                     /locus_tag="PKK76_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL68561.1"
                     /translation="LSHVLPRVSRGLRLIIVLNLAFAAGSWFLHFTIGLDIVATVLPG
                     LMIALATSAFGLAECIRGAIAGDRTSRLLVLAITCFAACAVFDSLVFAFAFAISKFVR
                     WNVQTTPIGFLILCGTLSYIVGNRYVEDRKKAELILIETQKNLIRSYQRFVPEQLFSL
                     MNRDSVIDVQLGDASQREMSILFSDIRSYTTISEALGPTENFAFLNGLMRITGPVIRN
                     HGGFIDKFIGDAIMALFPGPADDALNGAIGMKRAIEGYNAERRAQGQPPVSMGIGLNT
                     GRVILGTLGEAERMDSTVISDTVNLASRVEGLTKYYGVALLITHHTLVRLSDPTRYQY
                     RLVDRVVVKGKTEAVSIFEIFDGDADRETIVKINTQVDFERGVQEYHARNFSVAREFF
                     QKVIRTNPNDQVANIYLSRSEHFAKHGVPVDWEGISIMEGK"
     gene            complement(1327..1797)
                     /locus_tag="PKK76_08545"
     CDS             complement(1327..1797)
                     /locus_tag="PKK76_08545"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNL68562.1"
                     /translation="MKQTTTVVNEIDISISPEMVFDLVSSPGLWPRWHPSSRHLAPGA
                     DRPLKKGETFSEEIKAGGREGKLQWIVTECDRPRLWVATASSDQGVTIQLKYETEKNS
                     AGARFKRTLNYTMTSPFLILANTIFMRFKIRRESAHALGNLKQLLEKGVSSDVV"
     gene            complement(2112..2489)
                     /locus_tag="PKK76_08550"
     CDS             complement(2112..2489)
                     /locus_tag="PKK76_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNL68563.1"
                     /translation="MKIAGIVVRTLMGLLFIWGSIAFFFHMYPKPELTGNLKIFNEGI
                     DASGYLMVLVKSVELICGIAFLSGRFVTLASVVIAPIIVNILCVHIFLDHTGLPVAIF
                     LVLANGFLAYLNRENYKSLFVVK"
     gene            2737..3282
                     /locus_tag="PKK76_08555"
     CDS             2737..3282
                     /locus_tag="PKK76_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68564.1"
                     /translation="MTSRAAAVFPLFFFLLKVYQYRMPADQAQILWWCTISNLGLGLS
                     MLLGFARGVWIFSFMLILGTPIWLLDVWATGDFTIYSVFTHVISPALGIYSVRKLSVP
                     RGIWWQGMIYYFSLILIARALTPPALNVNLAHDVYAAAKPFIQNYWLYTAMNSILLAT
                     SLYVLGRLASQRFPLARGPRT"
     gene            complement(3318..5369)
                     /locus_tag="PKK76_08560"
     CDS             complement(3318..5369)
                     /locus_tag="PKK76_08560"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL68565.1"
                     /translation="MVRRVLVISLTIFALAGRVQADPVSTEERPIILTSDLRHRQLGR
                     EVDVLEDASGQLTIHDVKSGSARLRFRPGSSDVLSFGFTHSTYWIRARLENRSNENLW
                     FLQVAFPLIDHIELFEEERLVSQTGTQFPFSTRDMEHRTFVYKIKPASTTYYLRVQNE
                     DSMQIPLAVWSPEAFHSADHDEQMALGAYYGILIVMAAYNLFLFLSLRERGYFYYVIY
                     ITVFGFFLFSQYGFAYEYLWPNWTTLARKMNPVLAGALEASTLIFTRHFLNTRAHAPR
                     LDQVITVLIYLACLASPLALFLNLTHSAVMVVCLGLAASKCALVAGSLSLRAGFRPAR
                     YYMTAFLLLIVGAVLYALKTFGAIPVTFVSQYEMQLGSALEVTLLSLGLADRMNIMRR
                     QTETAQRQLLEEKSHSLERQTDLTRAYARMVPGEFLGILGRNSILEVKLGDSVQKTMT
                     VLFSDIRSFTELSETMTPRENFDFLNSYLRRMNPIIQRNGGSIDKYIGDAIMALFPQS
                     PEGAVRAAVEMQKALHEFNLHREERGFRPISIGIGIHTGNLMFGTIGGDERMEVTVIS
                     DAVNQASRMEGLTKAYGVGTIISETTFRELANPQDYSLRLLGSFSMKGKSNNVTLLEI
                     LDGLPAAEFELRKSTRSDFEHGVFSHLNGQLEEARSYFGKVLAVNPLDEAAKYYVSIC
                     K"
     gene            complement(5660..6022)
                     /locus_tag="PKK76_08565"
     CDS             complement(5660..6022)
                     /locus_tag="PKK76_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNL68566.1"
                     /translation="MNDNMLFLALGFFAGTISGLIGIGGGIILVPVLVFFFAFSQQQA
                     EGTTLAAMVPPIGALAAYVYYRAGFVNVPAAMWIAGGFIVGGFVGARFATSINPEMLR
                     RIFGVFAVAIGAKMLWGA"
BASE COUNT         1656 a         1634 c         1537 g         1493 t
ORIGIN      
        1 ctgagtcatg ttctcccgcg cgtcagccgg gggcttcgcc tgatcattgt tttgaacctc
       61 gcatttgccg caggatcctg gttcctccat ttcaccattg gcctcgatat cgttgcgact
      121 gtcctgcccg gattgatgat tgcgctggca acgtccgcct ttggcctcgc agaatgcatt
      181 cgcggagcga tagccggcga ccgcacgagt cgtcttctcg tgctcgcgat cacgtgtttc
      241 gccgcatgcg cagttttcga ttctctcgtg tttgcttttg cgttcgctat cagtaagttt
      301 gtgcgctgga acgtacaaac cacccccatt ggctttctga tcctttgtgg aactctgagc
      361 tatatagtcg ggaaccgcta tgtggaagat cgcaagaagg cggaactcat attgattgaa
      421 acgcaaaaga acctcattcg gtcctatcaa cgcttcgtac ccgaacaact attctcgctc
      481 atgaaccgcg acagcgtaat cgatgtccag ctgggggacg cttcacagcg cgaaatgagt
      541 attctctttt ctgatatcag atcctacaca actatctcag aagcactcgg gccaaccgaa
      601 aactttgcgt tcttgaacgg tttgatgcgc attacaggtc ccgtgatacg caatcacggc
      661 ggtttcattg ataagttcat cggagatgca atcatggcgc tttttcctgg accggcggac
      721 gacgcattga acggcgcgat cggcatgaag cgcgcaattg aaggttataa cgcggaacgc
      781 agggctcagg gacagccccc tgtctccatg ggaattggcc tcaacaccgg cagagtaatt
      841 ctcggaaccc tgggtgaagc cgagagaatg gacagtactg ttatcagcga tacggtaaac
      901 ctggcttcgc gggtcgaagg actgacgaaa tactatggag ttgcgctttt gatcacgcat
      961 cacaccctcg tgcggttaag cgatccaaca cgctatcaat accggctggt cgatcgtgtc
     1021 gtcgttaaag gaaaaacaga agctgtttcg atttttgaaa tcttcgacgg ggatgcagac
     1081 agagagacaa ttgtcaagat caatacccag gtcgatttcg aaagaggtgt tcaggaatac
     1141 cacgccagga acttctcagt ggcccgggaa ttttttcaaa aagtaatccg gacaaatccg
     1201 aatgaccagg ttgccaacat ctacctcagc aggtctgaac attttgcgaa gcatggcgta
     1261 cccgtagact gggaaggaat ctcgattatg gagggaaagt gaatgcagtg gcattgcgca
     1321 tttccactac acgacgtcgc tagagacgcc tttctcaaga agctgtttca gattgcccag
     1381 cgcatgcgcg gattcgcgtc tgattttgaa ccgcatgaag atggtgttgg ccaatatcag
     1441 aaatggactg gtcatggtgt aattcagagt ccttttgaag cgcgcgccgg ccgagttctt
     1501 ctccgtttca tatttcaatt gaatcgtgac gccctggtca ctgcttgctg tggcaaccca
     1561 tagacgaggt ctgtcgcatt ctgttacgat ccactggagt ttcccctctc tgccgcctgc
     1621 tttgatttct tctgaaaacg tttccccttt tttcaaagga cgatccgctc caggagcaag
     1681 gtggcgagaa gacggatgcc atctgggcca taggccgggc gagctcacca gatcaaagac
     1741 catctccggc gaaatggaaa tatcaatttc atttactacc gtagtggtct gtttcattta
     1801 taagttcttc taccgaggtt gcaggaaagg caagcagtat tgaaatgtcg gcaaccaatc
     1861 aagtccattg aattacaaaa tatatcatct cccgcacaca acctgaaaag tctctcagac
     1921 tcatgcagta cctgcagcgc ccgggtgcag tccacagcgg agttcggagc gtccgagcgt
     1981 cgcttcagcg cgtcgcgagg atggactgca tccgggcgtt gcagtttcta acacatgagt
     2041 cgcaagagac gccgaggatg gtttgcatcc cgggcgctgc agtttcttac tacatgagtt
     2101 gcaagagacg cctacttcac gacaaagaga gatttatagt tctctctgtt cagatatgca
     2161 aggaagccgt ttgcaagaac gagaaaaatc gcaactggaa gcccggtgtg atctagaaag
     2221 atgtgcacgc acaaaatatt cacgatgatt ggtgcgatca caacgcttgc cagagtcaca
     2281 aatcgacctg aaagaaacgc aatcccgcaa atcaactcaa cggatttgac aagaaccatc
     2341 aaatatccgg atgcatcgat tccttcgttg aagattttca agttacccgt gagttcgggc
     2401 tttggataca tgtggaaaaa gaacgcgatc gacccccaga taaaaagtaa tcccatcagc
     2461 gtacggacaa ctatgccagc aattttcatt tcgaaatcct ctacggcaac catttgaaat
     2521 ggagggcgtg cttacaattg ttttgcgaga cgattgcatg ttttttccgc gtgattcatg
     2581 actcgatttt tcgtgcccgg cggccgccgc ataacacgcg ttagcttaca atctccttct
     2641 cgatcactca aggggtgaaa ccacaagcaa cgagaacgcc ctggaggact ttttttgatt
     2701 gcacgcaagg cgcaaacgca tggccaatga aacacgatga catctcgtgc agccgccgtt
     2761 ttccccctgt tcttctttct gctgaaggtt tatcagtaca ggatgcctgc agatcaggca
     2821 caaattctat ggtggtgcac aatttccaat ctcggactcg ggctatccat gcttctcggt
     2881 ttcgcgcgag gcgtctggat tttttcattc atgttgatac tgggaactcc catctggctt
     2941 ctagatgtat gggccacagg cgatttcacc atctattccg tattcacgca tgtgatcagc
     3001 cccgctcttg gaatttatag cgtaaggaaa ttgtcggtac cgcgcggaat ctggtggcag
     3061 ggaatgatct actacttctc tctcattctc atcgcgcgag cccttacacc gccggctctg
     3121 aacgtaaacc tcgcacacga cgtttacgcc gcggcaaaac ctttcattca gaattactgg
     3181 ctgtatacgg cgatgaattc tattctgctt gctacgtcac tttatgttct gggaaggtta
     3241 gccagccaga ggtttcctct agcgcgaggc cctcgcacat aacgttaggc gactcactct
     3301 gacttttcac cctgacttta tttacaaata cttacgtaat atttcgctgc ctcatccagg
     3361 ggattgaccg cgaggacttt gccaaaataa gagcgagcct cttccaactg cccgttcaaa
     3421 tgactgaaga caccgtgctc aaaatctgat cgcgttgact ttcgcaattc aaactccgcg
     3481 gctggaagcc catcgaggat ttcaagcaag gtaacgttgt tgctcttgcc tttcatggaa
     3541 aaagaaccaa gcaatcggag ggaatagtcc tgaggatttg caagttcgcg gaatgttgtt
     3601 tcactgatga tggttccaac gccgtacgcc tttgtgagtc cctccatacg cgacgcctgg
     3661 ttcacagcgt ccgaaatcac cgtcacttcc atcctctcat cgccgcctat cgttccgaac
     3721 atcaggtttc ctgtgtgaat gccaatcccg atgctgatcg gtcgaaaacc tctttcctcc
     3781 ctgtgaaggt tgaactcatg cagggccttc tgcatttcta ccgcggcgcg aaccgcacct
     3841 tccggggact gcgggaacaa agccatgatc gcatccccga tgtatttatc gatggaccca
     3901 ccgttgcgtt gaatgatggg attcatgcgc cggagatagg aattcaggaa atcaaaattt
     3961 tcgcgcggcg tcatggtctc agaaagttca gtgaaagatc gaatatcaga aaatagaacc
     4021 gtcatcgttt tttgaacgga atcgcccagc ttcacttcca gaattgaatt gcgaccgagt
     4081 atacccagaa actccccggg caccattcgc gcgtacgcgc gagtcaaatc ggtctgcctc
     4141 tccagcgaat gcgatttctc ttccagcaac tgcctctgtg cagtctccgt ctggcgccgc
     4201 ataatattca ttctgtctgc cagaccgagc gaaagaagag tcacttccag tgccgaaccc
     4261 agctgcattt catactgcga aacaaacgtc actggaatgg ccccgaatgt ttttagcgcg
     4321 taaagtaccg cgccaacgat gagcaacaaa aaagcagtca tgtaataccg cgcaggacga
     4381 aatccggcgc gcaaactcaa gctacccgcg accagagcac acttgcttgc agcaagacca
     4441 agacaaacga ccatcacggc cgaatgagtc agattcagaa acaacgccag gggagacgca
     4501 agacacgcca aatagatcaa gaccgtaatg acctggtcga ggcgcggagc gtgagcccga
     4561 gtgttcaaga aatgacgggt gaaaatcaga gtgctggctt ccagagcacc ggcgaggact
     4621 ggattcattt tgcgtgcgag agttgtccag ttcggccaga gatattcgta tgcaaagcca
     4681 tactgcgaaa acaagaagaa tccaaagact gtaatgtaaa taacgtagta gaagtatcct
     4741 ctctcccgga gcgaaagaaa tagaaagagg ttgtaggccg ccatgacaat caagatgccg
     4801 tagtaagctc cgagtgccat ctgttcatca tgatcggctg agtgaaaagc ctccggagac
     4861 cacacggcaa gagggatctg catgctgtct tcgttttgaa cgcgtaaata gtaagtcgta
     4921 gaagccggtt tgattttgta gacgaacgtg cgatgttcca tgtcgcgagt ggaaaacgga
     4981 aactgagttc cagtttgtga tacgagtctc tcttcttcaa agagttctat gtgatcaatc
     5041 agagggaaag ctacctgcag aaaccacaga ttttcattgg aacgattttc aaggcgcgcc
     5101 cgaatccagt atgtggaatg agtgaaacca aagctcaaaa catcagagga accagggcga
     5161 aaacggagtc gcgccgatcc actttttaca tcgtggatag tcaactgtcc ggacgcatcc
     5221 tcaagcacgt cgacctcacg acccagctga cgatggcgaa gatcgcttgt aagaatgatg
     5281 gggcgctcct ccgtagaaac cggatcagct tgcacccggc cagccagagc aaagatggtc
     5341 agtgagataa ccagaaccct gcgaaccaca ctttgtgtct agttctagaa catgaaggag
     5401 caaccagaat ttcggtttca tgaattttat tttggttcgc tggttgtaaa taattcgaat
     5461 cgaaaagaaa attcaagtaa tgaaataggt gtccatccga ctaaagcccg acggagttct
     5521 gcttgatact cgattggaac cataacgagc ccggtgtctg aaagatattt gtatagaaat
     5581 attactattt catcctgagg cagaacagat ctcgctcaca gagccagaca cggtcgatcc
     5641 agattcagcc cctgaaacat caggctcccc agagcatctt cgcgccaatg gcaaccgcga
     5701 agacaccaaa aatgcgtcgc agcatttcag ggttgatgga ggtcgcgaac ctcgcaccca
     5761 caaagccgcc cacaatgaat ccgcctgcga tccacatcgc cgccggcacg tttacgaacc
     5821 ctgctctgta gtatacatac gcggcaagag ctcctatggg aggaaccatc gcggcaagag
     5881 tcgttccttc cgcctgctgc tggctgaagg cgaaaaagaa aaccagaaca ggtacaagaa
     5941 tgatccctcc gccaattcca attagaccac tgatcgtacc cgcaaagaat ccaagagcca
     6001 ggaaaagcat attatcgttc atgtaagcta tacggctgtt gccatgcgaa agcgacagga
     6061 ttttagggtt cacgcatcga cggccggttc ggtcgcaatt gccacaggga agctgcgtgg
     6121 cacggcctga aatccttgga caaaatgaac gttcgccttc gccttaccct cgctaaatat
     6181 taaagagagt cgttgactac caaaagtgcc caaagagaaa gacgtattca acgggacaag
     6241 cgaatgccgt ctaacgcgaa gttactggaa atcattcacc tccaaacaga aatcgccaag
     6301 ctaggaatcg acctgagcca
//
LOCUS       DAORFF010000176         6245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6582_length_6245_cov_3.832795, whole genome shotgun sequence.
ACCESSION   DAORFF010000176 DAORFF010000000
VERSION     DAORFF010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6582_length_6245_cov_3.832795"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="PKK76_08570"
     CDS             <1..1255
                     /locus_tag="PKK76_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNL68567.1"
                     /translation="TLKTISPGKYIANGRKYYIGNANKASFVSTFAKDAETGDYVFFA
                     AESAHPNYTCVQNVVNSQNYVAEYELKDYPITDKEILSRGRDAWDASLATIAYCKYNL
                     GWASIGICTHAFYEAINHAANRVLFKHKVTDFPHIKQFMTDAWTRLLAMRLFSFRASD
                     YMRAASEDDKRYLLYNPMVKMKVTIQGEEVVNLLWDVIAAKGFEKDGYFEMAARDIRG
                     LPKLEGTAQVNMVLIMKFMDKFFFEPSPYPDLPRQKSPGNDSFMFAQGSTSKGQNRIQ
                     FHDFNIAYNQSKLPNVKIFQSQIEVFKKFLKEAAPDKAQTRDLDFMLNVGELFTLVPY
                     GQLILECAKSEGISDDLVDQIFDFMVRDFSRYALQMYSRTSSTQAQMDLCMQMIKKPA
                     VNTERFERVWKENVLSLNMAYVMKP"
     gene            1298..2431
                     /locus_tag="PKK76_08575"
     CDS             1298..2431
                     /locus_tag="PKK76_08575"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL68568.1"
                     /translation="MGKRSALCAAFAVMAALSHCAARSECSALDISCGLPAYLLMLQS
                     RQFAYIANASTFEAFRYDQNTSQLTLLQTVASGQRCDAVRPHPYGVFLHCVVDVAGTD
                     YLRVFRIGSGGVLSFIEEFNIGSVTGLGLANHHRGDIVVLIVDTFSRYYLYQMDQITG
                     HLTLASQSPAGVGGAASAMSRDGNFIYGRTSGASQVTLRITGNSITQMQDLGNPGTNT
                     FTFTTTPDGFYQLQNINQALGIRRYTVNGDGTLSAPLDYPNSAQSEIFDDDSWTPDGR
                     FFYTRSGASADNLRGYAYSGGNISLVTGTPIVLPGGLGTPTVDPGGKYVFIDRSAAGL
                     YHVFQINRITGELTQMADSPRACTSNPCGRISFGTRARFPGDF"
     gene            complement(2432..3109)
                     /locus_tag="PKK76_08580"
     CDS             complement(2432..3109)
                     /locus_tag="PKK76_08580"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNL68569.1"
                     /translation="MRKLTIVFFLLCAFACKGPSQPDAGLFKNVQGDVTVERSGKVEP
                     AKVGDRFVPGDTVRTGENGIAIIAIKGTVGQAELQPNSVFRLNLGDQKTEVLLDKGNM
                     WLQVQKATSREEFTVRTPTTVAGVRGTKFYTFQNGDTVGVCYCEGDVDFAAGDSKSNT
                     YHKDTLVFTRNGKTVSLTADEVPGVTYEHNHSLMDGSPLGQQSKLTPEMKKKIFDAAE
                     KKFKELK"
     gene            complement(3176..3466)
                     /locus_tag="PKK76_08585"
     CDS             complement(3176..3466)
                     /locus_tag="PKK76_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNL68570.1"
                     /translation="MKHFMIEVTYTVPIERIDEVTAEHRAFLQKWYDEGTLLMSGPQV
                     PRTGGIILGKCESREKMEEIFSQDPFKLKGCAMYRIVEFNPVKHQEFLKTWL"
     gene            complement(3463..4431)
                     /locus_tag="PKK76_08590"
     CDS             complement(3463..4431)
                     /locus_tag="PKK76_08590"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNL68571.1"
                     /translation="MILVVEDSPDQQRLVRQMLETAGYRDVRTCDDAPEALEIVRKNI
                     DQMEAVITDVWLIDGSGIQLTRDIRKIDQLRDLPVIIMTGKEGPEILPQAVEAGVNDF
                     LFKPFHWMELIARIRNNLRLKSEIARRVAREIELAAAKDELREALSRFEEVSHQDALT
                     GVSNRRKMDEHLEREWRLAQRARRPVSVVLIDVDYFKEYNDQYGHPQGDNCLRIIARV
                     VANGVHRPADLVARYGGEEFILILPETPLEGAAHVAEVIRAAIEAAQIEHKGSPLGVV
                     TASLGVAALLPEKGYEPVTLIQNADRALYQAKKERRNMVSVSREAL"
     gene            complement(4437..5255)
                     /locus_tag="PKK76_08595"
     CDS             complement(4437..5255)
                     /locus_tag="PKK76_08595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNL68572.1"
                     /translation="MILTFPALDRIRSINSGAKITIAANPLYAQAALEFRLVDEILSF
                     EGRELRPLFIGQTANIHFDEAYVWMKDVDDLFVQSLRKICPVYASPSIQSHPFVHQSE
                     FLFRIHGADVSASEIARVWASLPGRLPIRSEEFDVCIHPGSAGIHKRWALERFRAVRD
                     SLVQSGRRVSWILGPAENELLESGIVMSGSEDTIFRSPSIRELIGILSSCKVFLGNDS
                     GVSHLAAAAGAPCIVIFGPTDPCIWAPRGNVQILHTLDASVQDVLDLLKSARRH"
     gene            complement(5294..>6245)
                     /locus_tag="PKK76_08600"
     CDS             complement(5294..>6245)
                     /locus_tag="PKK76_08600"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HNL68573.1"
                     /translation="PNGTIRNILDGTVFREPIVCKNVPRLVPNWTAPICIGRHAFGDQ
                     YRATDFVTKGKGKLTITFTPEGGAPQTHEVFDFKGDGVALAMYNTDESIRGFAYSCFN
                     MALTKKWPLYLSTKNTILKKYDGRFKDIFEEIYQKEFKKAFEAAGTVYEHRLIDDMVA
                     SALKWNGNFVWACKNYDGDVQSDTVAQGFGSLGLMTSVLVTPDGKTMEAEAAHGTVTR
                     HYREHMAGKPTSTNPIASIFAWTRGLEFRGKLDNNPDLVKFCQALEQVCVETVESGKM
                     TKDLAVCVYGNKVNHGEHFLYTEEFLSALDTNLKAKLGYK"
BASE COUNT         1574 a         1632 c         1491 g         1548 t
ORIGIN      
        1 gactctcaaa acaatttccc cgggcaagta cattgcaaat ggtcgtaagt actacatagg
       61 aaatgcaaac aaagcctctt ttgtttccac tttcgcaaag gatgcggaaa ccggagacta
      121 cgtgttcttc gcagcagaat ctgcacaccc gaactacacg tgtgtgcaaa acgtagtgaa
      181 ctcccagaac tatgtagctg agtatgagct taaggattac ccgattacgg acaaagaaat
      241 tctatcgcga ggccgtgacg cctgggacgc atcccttgcc accattgctt actgcaaata
      301 caacctggga tgggcgtcca tcgggatctg tactcatgca ttttatgaag caatcaacca
      361 cgcagccaat cgtgttcttt tcaaacacaa ggtcacagac tttccacaca tcaaacaatt
      421 catgacggat gcgtggactc gcttgctcgc gatgagacta ttttcatttc gcgccagcga
      481 ctacatgcgt gcggcgtcgg aagacgacaa acgctatctt ctctacaacc ctatggtgaa
      541 aatgaaagtc acaattcagg gagaagaagt cgtgaacctg ctatgggatg tgattgcggc
      601 aaaaggattt gagaaagacg gctattttga aatggctgcg cgagacatcc gcggattgcc
      661 taaactagaa ggaaccgcgc aggtcaatat ggtattgatc atgaagttca tggataagtt
      721 tttctttgaa ccatctcctt atcccgatct gccgcgtcag aaatctcccg gcaacgattc
      781 gtttatgttt gcgcagggaa gcacgagtaa gggacaaaat cgcattcagt ttcacgactt
      841 caatatagca tacaaccaat ctaagcttcc aaatgttaag atcttccaat cgcaaattga
      901 agttttcaag aagttcctga aggaagcagc accggacaaa gcgcaaacac gtgacctgga
      961 tttcatgctc aacgtgggag agctcttcac actcgttccg tatggccagc tgatcctgga
     1021 gtgcgcgaaa agcgaaggga tttcagacga ccttgtagat cagattttcg atttcatggt
     1081 gcgcgatttt tcacgttatg cgcttcagat gtattccaga acttccagca ctcaagcgca
     1141 aatggatctc tgcatgcaaa tgatcaagaa gccggcagta aacacagaaa gattcgagcg
     1201 cgtgtggaaa gagaatgtat tatctttgaa catggcctac gtcatgaagc cgtaaaaaga
     1261 ttgcgtcaga ttcaccttgt ccgctgtata gcccccaatg ggcaaacgaa gcgcattgtg
     1321 cgctgccttc gcagtgatgg cagcactctc tcattgcgct gcgcgttcag aatgtagtgc
     1381 cctggacatt tcgtgtggct tgcctgcata cttgctcatg ctacaatcca ggcaattcgc
     1441 ctacatcgca aacgcttcca ctttcgaagc ctttcgctac gatcaaaata catcccagct
     1501 cacattgctg caaacggtcg cttccggcca aaggtgcgat gcggtgcgcc cccatccata
     1561 cggtgtcttc ctccattgcg tcgtggatgt ggcaggcacg gactacctgc gcgttttcag
     1621 aatcggcagc ggcggagttc tctcttttat tgaagaattt aacattggct ctgtaacggg
     1681 ccttggtctg gcgaatcatc acagagggga cattgtcgtc ttgatcgtgg atactttttc
     1741 tcgatactat ctctaccaga tggatcagat caccggtcat ctcactctag cgtctcaatc
     1801 ccccgccggc gttggcggag cggccagtgc tatgagtaga gacggaaact tcatatatgg
     1861 aagaacttca ggcgcaagcc aggtgactct tcgaatcaca gggaatagca ttacgcaaat
     1921 gcaagatctg ggcaatcctg gaacgaacac gttcacattc acaacgactc cggacggatt
     1981 ctatcaactc cagaacatca atcaggcgct cggtatacgg agatacacgg taaacggaga
     2041 tggaacgttg tctgctcctc tcgattaccc gaactctgcg cagagcgaaa tcttcgacga
     2101 tgactcgtgg actcctgacg gtcgtttctt ttacacgcga agtggcgctt ccgccgacaa
     2161 cttgcgcggc tatgcatatt caggcggaaa catcagcctc gtcactggaa cgcccatcgt
     2221 actgcccgga ggcctgggaa ccccgaccgt ggatccaggc ggcaagtatg tattcatcga
     2281 tcgaagcgca gctggtttgt accatgtatt ccagatcaat agaattacgg gagaattgac
     2341 gcaaatggcc gattctcctc gagcgtgcac atccaacccc tgcggcagaa tttcgtttgg
     2401 gacgcgtgca cgctttcctg gagatttcta actacttaag ttctttaaac ttcttttccg
     2461 ctgcgtcaaa tatcttcttt ttcatctcag gtgtcagttt gctctgctgg ccaagtgggc
     2521 ttccgtccat cagagaatga ttgtgttcat atgtgacgcc tggcacttca tccgccgtga
     2581 gactgaccgt cttgccgttg cgcgtgaaga cgagagtgtc cttgtgatag gtattggact
     2641 tagaatcccc tgcggcaaaa tcaacatcac cttcacagta acagacgccc acagtatctc
     2701 cattctgaaa tgtatagaac ttcgtgccgc gcacaccggc cacagtcgta ggcgtgcgca
     2761 ctgtgaactc ttcgcgcgaa gttgctttct gaacctggag ccacatgttg cccttatcga
     2821 gtagaacttc tgtcttttga tctcccagat tcaatctaaa cacgctgttt ggctgcagct
     2881 ctgcctgccc tacagttcct ttgattgcaa tgatcgcaat tccattctct ccagttcgta
     2941 cggtatcccc tggaacaaac cgatctccca ctttcgcagg ctccacctta ccggaacgtt
     3001 cgacggtcac gtctccctga acattcttga ataatccggc gtccggttgg gaaggacctt
     3061 tgcacgcaaa ggcacagaga agaaaaaaga caatcgtgag tttacgcatg agagcaagtt
     3121 cagatagaga acaaaaaatc acaactaata aaaacgcgaa cgcggaaaga agagactaca
     3181 gccaggtctt gagaaattcc tgatgcttta cagggttgaa ttcaacaatg cgatacatcg
     3241 cgcagccctt gagcttgaat ggatcttgag agaaaatctc ttccatcttc tctcgcgatt
     3301 cacacttacc cagaatgatg ccgccggttc tcggaacctg gggtccagac atcaaaagag
     3361 tgccttcatc ataccatttc tgcaaaaacg cccgatgttc tgccgtgact tcatcgattc
     3421 gttcaattgg aacagtgtat gtaacttcaa tcatgaaatg cttcataatg cctcgcgcga
     3481 aacagaaacc atatttcttc tttctttttt tgcctgatag agcgcacgat ctgcattttg
     3541 aatgagcgtt acaggttcat atcctttttc aggaagaaga gctgccacac caagactcgc
     3601 cgtgaccaca ccaagagggg atcctttgtg ctcaatctgt gcggcttcaa tggcggcgcg
     3661 aatcacttcc gcaacgtgcg ccgcaccttc caatggggtc tcaggcagga tcaagataaa
     3721 ttcctctccg ccgtaacgcg ctacaaggtc tgccgggcga tgcacaccgt tggcaacgac
     3781 ccgggcaata atgcgaagac agttgtctcc ctggggatgc ccgtattgat cgttgtattc
     3841 cttaaagtaa tctacatcaa taagcacaac gcttacagga cgacgcgctc tctgagccag
     3901 gcgccattct ctttccaggt gttcgtccat ctttcgccga ttggagacgc ctgtaagtgc
     3961 atcctgatgc gaaacttcct caaagcggct gagggcttcg cgcaattcat ctttggccgc
     4021 agcaagctca atctctcgcg caacacgccg cgcaatttca gatttcaatc tcagattatt
     4081 tcgaatgcga gcaatcagtt ccatccagtg aaatggctta aatagaaaat cgttgactcc
     4141 cgcttccact gcctgcggca aaatctcggg accttccttt ccagtcatga tgatcacagg
     4201 gagatcccgc agctgatcta ttttgcgaat gtctcgggtc aattgaatgc cgcttccatc
     4261 aatcaaccag acgtcggtga tcactgcctc catctgatcg atgttcttgc gcactatctc
     4321 aagggcctct ggtgcatcgt cgcatgtgcg aacatctctg taaccggctg tctccagcat
     4381 ttgtctcacc agtctttgct ggtcaggcga atcttctaca accaggatca tgctttctaa
     4441 tggcgccggg cggacttgag gaggtcaagc acgtcttgaa cagaagcatc cagagtatgc
     4501 aggatctgca cattgccacg aggagcccag atgcaaggat ccgttggacc aaaaatcaca
     4561 atacagggtg cacccgccgc cgcagccagg tgagaaacac ccgaatcgtt tccaaggaag
     4621 accttgcagg aggaaaggat tccaatcaac tcccgtatgg aaggacttcg gaagatggta
     4681 tcttcactcc cggacatgac aatgccagac tcaaggagtt cattctctgc cgggcccaga
     4741 atccaggata cgcggcggcc actttgtacc agggaatccc ttacggccct gaaccgttcc
     4801 agggcccatc tcttgtggat cccagcggat cctggatgaa tgcaaacatc gaactcttcc
     4861 gatcggatag gcagtcgacc gggcagtgat gcccagaccc tggctatctc agacgcggac
     4921 acgtccgcac catgaatgcg aaaaagaaat tcggactgat gaacaaatgg gtgcgattgg
     4981 atagaagggc tggcatacac cggacaaatt ttgcgaagcg actgaacgaa caaatcatcc
     5041 acgtccttca tccagacata cgcttcatca aagtgaatat tcgccgtctg accaatgaac
     5101 aaaggccgca gttccctgcc ctcgaaagaa aggatctcgt ctaccagacg gaattcaagt
     5161 gccgcctgag cgtataacgg gtttgcagcg attgtaattt ttgcgccgct gtttatagaa
     5221 cggatgcggt ccagagcggg aaacgtgagg atcacgtctc cgagagctcc gggccgcact
     5281 acgaggatcg tttttatttg tagccgagtt ttgctttgag attcgtgtcc agtgcggaca
     5341 agaattcttc agtgtagagg aaatgctctc cgtgattgac cttgttgcca taaacgcaaa
     5401 cagcaaggtc cttggtcatc ttcccacttt ctacagtctc tacgcagacc tgttccagcg
     5461 cctggcagaa tttcaccaga tctggattgt tgtccagttt gccgcggaat tcaagaccac
     5521 gagtccatgc gaagatggat gcgattggat tggtcgaagt aggcttgccg gccatatgtt
     5581 cgcggtaatg ccgcgtaacg gtcccgtgag ctgcttctgc ttccatggtc ttgccgtccg
     5641 gcgttacaag cacagaagtc atgagtccaa gagaaccaaa gccttgcgcg acggtgtcag
     5701 actgcacatc cccatcgtag tttttgcaag cccatacaaa gttgccattc catttgagcg
     5761 cggaagcaac catatcgtca atgagacgat gctcatacac tgtaccggct gcttcgaagg
     5821 cctttttgaa ttctttctga tagatttctt caaaaatgtc tttgaatctt ccgtcatatt
     5881 ttttcaggat tgtattcttc gtagagagat acagaggcca cttttttgtg agcgccatgt
     5941 tgaagcagga atacgcaaag ccgcggatag attcatccgt gttgtacatt gccagagcaa
     6001 cgccgtcgcc tttgaaatcg aagacttcgt gtgtctgggg tgcgccacct tccggcgtga
     6061 atgtgatggt gagctttcct ttccctttgg ttacaaagtc tgtcgcgcgg tattgatccc
     6121 cgaatgcatg gcggccaatg caaatgggag cagtccagtt tggaaccagg cgcggaacgt
     6181 ttttacaaac gatgggttcg cggaaaacag taccatccag aatgttccga attgtgccgt
     6241 taggc
//
LOCUS       DAORFF010000177         6242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6589_length_6242_cov_3.343462, whole genome shotgun sequence.
ACCESSION   DAORFF010000177 DAORFF010000000
VERSION     DAORFF010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6589_length_6242_cov_3.343462"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="PKK76_08605"
     CDS             complement(<1..1137)
                     /locus_tag="PKK76_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNL68574.1"
                     /translation="MALATSDSNPPASPGFSITLRLFRYLFRYKTRIVFGVLFSVLVS
                     LSNLVSITALVPIFNAIGETGPIEVLPCGGEEKERYQRMERGEQLDLIEKAQVAWTGV
                     KLAANKKAEGKSSREVVMALCAFVLPVYLLKLLCLTISFYCIGTAGLMAVRDLRMELY
                     NKLNVLGLEHFTENQTGFIMSRVINDAEAVGRSLSAEFQEGLVDLLYIITHFALLAVI
                     SWQMLLMALLIVPILSSPVAKFAKKIRGAAMGQQERLADLGAHIHEILSGIRVIRAFS
                     MQKFELGRFQQVNQKLYQNTFRGHYYHQVGPALTEFVATIAVVGFLSWGAYRIAASTM
                     NKGQFFTFFFTLIFLMRPIKQVSVLANLTSVARAAGQRIFEVLDK"
     gene            complement(1112..4243)
                     /locus_tag="PKK76_08610"
     CDS             complement(1112..4243)
                     /locus_tag="PKK76_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNL68575.1"
                     /translation="MSKRINNIVSRLKGKAVWIAVLIPVFVFPLLPDRTQFVGKVIKD
                     IRYEGLRNVSPDEITGLLTLERGTKVDDATLNADIKALFKSGYFGKISLRGVIEQDGV
                     ILIFEFSELPRVREVDFVGTDKLYAADLKSLVGFKEGEVYAEQKVKSAIPRIKEKYRS
                     EGYFLTEIWYRVSQLDENNEVTVKYIIDEGENIPIGKINIIGARHLDPEDLIGVLEQK
                     EEGTIEDGIFDETKFEQDKFKILGYAKAQGYVDADLDPTGTGYEIRWRSPTKPELGRV
                     VVVTYKLIEGDIRYFGGYSLEFDNDALNEELNPRERPRKNPKPQPVFRKEDLLASMEF
                     SDNDVGDLFDEGKYFRDRTLIQESYSTQGYVFSQVQPGFVNFSLTEETIKKYKNCKTI
                     ANPANDLERKCKKESEWLPLQAMEDHLKKHPEERGRVLRHVHYTVRENNLAYIENIIV
                     KGMVKTQERVIRRELLVKEGHLFNSALVNRSREKIYNLGYFKEVNLQMRPGSDDQKLN
                     LIIDVQEQPTGTISLGGGYGTQSGFSVFTEVGENNLNGTGQKISGKLQYGPLTKQISF
                     QWTDPWIYEACDDSTGSFWRNKQKQFDGAADGESIEKIAASLQNNYSEIGKIIRGYTE
                     SVKSDKSIEALDLTKVKIRRLLRKFVADEEECYRSIPRPWALSLYASYATETIRASSL
                     RISDDSNDLFEGSQYEVSSVGVGAGISHTFWLNWAHYHRYSPQWSIASRPTSLAENEI
                     LRRVGLGWQFKSALTNGLIYDTRDNVFNPTQGLNLDMSIEFVGQMLGGQDHYNQYTIN
                     GKYYMWWFDYTFGGLFRKQALKRWRVVSEFRASATFTHETAPFRGHQNKEINPFIEPQ
                     DKLFLGGYESLRGYDFRTDRNFPLPWQDGGSHMFLAGTELRFPIEPTLVWLAAFLDAG
                     SLYDNVGEFTGDTKTYANNYREIVAVNNARLDAGQLYFLERYSPYNFARYPYDSYYDW
                     NDPLRAVLSERNVSLDRMLFSWGFGVRIQIPVLPLRLFLAQKLYHTSGLKLKPIPGDS
                     KFQFVFGIGDFRF"
     gene            complement(4324..5943)
                     /gene="recN"
                     /locus_tag="PKK76_08615"
     CDS             complement(4324..5943)
                     /gene="recN"
                     /locus_tag="PKK76_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNL68576.1"
                     /translation="MLTELRIENFGIMDEMRFHPGDGLSVLTGETGAGKSLLIQSISA
                     LLGGRLGAGVVRAGAARALIEGRFREKDEDEIVLRREVSADGRSRVFINGTQTNLAGL
                     KARATSLIEMHGQHDQQRLLEPDHQLESLDAYCESSQLRERVASFYHEWQGLSKKLRN
                     VRMQKEERDYRLEYLHHSVKEIDQLSPEEGELERLTQEKALMQNGGKVAQDLHVASLL
                     LGGEEHAVLTGVTRIQQLLDRHDQIIPEFAGSLSDLNEACILLENFHDAIRQQMDRLQ
                     FSPERLEDVEERIQAYRRLIKKHGHSLARVRDDFLKEIHELANVEENEKKLEQNLADS
                     WGRLVEAAELLSRLRRSCIPGLEERMARELSALGMPGAEIRISIHREMSGEEEGTLVI
                     GEKGLDRVEFYFKPNPGEPAHPLRKIASGGELSRIMLACKSVMMEARPVSLVVFDEVD
                     AGVGGEIAHTLAERLRHIARDCQVLVVTHLAQVASRADHHFKVAKKQENGRTVSRIQR
                     LQSEHRVQEVARMMGGARALEFARDCLSMAG"
BASE COUNT         1484 a         1676 c         1477 g         1605 t
ORIGIN      
        1 cttatccaga acttcgaaaa ttctctggcc cgccgcgcgc gcaacgcttg tcagattggc
       61 gagaacggat acctgcttga tgggacgcat cagaaagatc aaagtgaaga agaaggtaaa
      121 gaattggcct ttgttcatgg tgcttgcggc gatgcgatac gcaccccacg acaaaaagcc
      181 aaccacagcg atggttgcta caaactcagt aagagcgggg ccaacttgat gatagtaatg
      241 tccgcgaaat gtattctgat agagtttctg attcacctgc tggaatcttc cgagttcgaa
      301 cttctgcatg gaaaatgcac gaatgacccg aatgccgctc agtatttcgt gaatgtgtgc
      361 acccagatcc gcaagcctct cctgctggcc catggccgca ccgcgaatct tctttgcaaa
      421 tttcgcaaca ggcgaagata gaatgggcac aatcagaaga gccatcagca gcatctgcca
      481 ggaaatcact gcaagcaagg cgaaatgagt gatgatgtag agcaaatcaa cgaggccttc
      541 ttgaaattct gcggaaagac ttctgccgac tgcctccgcg tcattgatga cacgactcat
      601 gatgaatcca gtctggttct ccgtaaaatg ctcgagcccc agaacgttga gcttgttgta
      661 aagctccatg cgcaaatcgc gcacagccat cagtcccgcc gtaccgatgc aatagaagga
      721 aatggtcaga cacagaagct tgagtaagta cactggcaga acaaaagcgc ataacgccat
      781 aaccacttcg cgcgaactct ttccttctgc ctttttattc gccgccagct tcactccggt
      841 ccacgcgacc tgagcctttt cgatgaggtc gagctgctcc cctctctcca tccgctggta
      901 tcgttctttc tcttctccgc cgcaggggag gacttcaatg ggccctgttt ctccgattgc
      961 attgaaaatc ggaacgaggg ccgtgatgga aaccagattg gatagcgaaa ccaggacgga
     1021 aaatagcacg ccgaaaacaa tccgcgtctt atagcgaaac aaatagcgaa acagcctcag
     1081 cgtgatgctg aagccaggcg aagcaggtgg gttagaatcg gaagtcgcca atgccaaaca
     1141 caaactgaaa tttggaatca cccggaatcg gcttcagctt caacccagaa gtgtgataga
     1201 gcttctgagc caggaacaat cgcagaggca gaaccggaat ttgaatcctg actccaaaac
     1261 cccatgagaa caacattctg tcgagcgata catttcgctc cgacagaacg gcacgaagcg
     1321 ggtcgttcca gtcataatac gaatcatacg gataccgggc gaagttgtag ggactgtagc
     1381 gttccaggaa atacagttgt cccgcgtcaa gcctcgcatt gtttacggcg acaatctcac
     1441 gatagttatt tgcataagtt ttggtatcac ccgtgaactc gcccacgtta tcgtaaaggc
     1501 tgcccgcatc cagaaatgcc gcgagccaca caagagttgg ctcaatcggg aaacgtagct
     1561 ccgttcctgc caggaacatg tgggagccac cgtcctgcca tggtagaggg aaattcctgt
     1621 ccgtgcggaa atcgtatccg cgcagcgatt catatcctcc gaggaacaac ttgtcctgcg
     1681 gttcaatgaa tggattgatc tccttattct gatgccctct aaacggcgcg gtctcatgcg
     1741 tgaatgtggc agatgcgcga aactcagaaa ccacgcgcca tctcttcaac gcctgcttgc
     1801 gaaacagtcc gccgaacgta tagtcaaacc accacatgta gtacttaccg ttgatggtat
     1861 actgattgta gtgatcctga ccacccagca tctgtccgac aaattctatg gacatatcca
     1921 gattcaagcc ctgggtagga ttgaagacgt tatcacgcgt atcgtaaatc agaccgtttg
     1981 taagagctga cttgaactgc cagccaagcc cgactcttct gagaatttcg ttttccgcta
     2041 gagaagttgg gcgcgatgca atggaccact gcggcgaata acggtggtaa tgcgcccagt
     2101 tcagccagaa cgtatgcgaa atacctgcgc ccactcccac agatgatact tcgtactggg
     2161 atccttcaaa aagatcattg gaatcgtcgc taatgcgcaa agaagaggcg cgaattgtct
     2221 ccgtcgcata actggcgtaa agcgaaagag cccatggcct tggaatactt ctgtaacatt
     2281 cctcttcatc ggcgacaaac ttgcgaagca atctccggat cttcaccttg gtcaggtcga
     2341 gagcttcaat cgatttgtcc gattttacgg actctgtgta cccgcggatg atcttaccga
     2401 tttcgctgta gttgttctga aggctcgctg ctatcttttc aatggactca ccgtcggcgg
     2461 ctccatcaaa ttgcttttgc ttatttctcc agaagcttcc ggtcgaatcg tcgcatgctt
     2521 cgtaaatcca gggatctgtc cactgaaagc taatctgctt ggtgagcgga ccgtattgca
     2581 gtttgcctga aattttctgg cctgttccgt tcaggttgtt ttcgcctact tctgtgaaga
     2641 cagagaatcc agattgtgtt ccgtatccac cgccgagcga aatggttccg gttggttgct
     2701 cctgcacgtc aatgatcagg ttcagttttt gatcatccga gccaggtcgc atttgcaggt
     2761 ttacttcttt gaagtaaccc aggttgtaga ttttttcgcg gctccgattc accagagcag
     2821 agttgaaaag atgcccttct ttcacaagaa gctcacggcg aatcactctc tcctgggttt
     2881 tgaccatacc ctttacgatg atgttttcaa tgtaggctag attattttcg cgcactgtgt
     2941 agtgcacatg gcgaagtaca cgaccacgct cctcaggatg tttcttgaga tgatcttcca
     3001 ttgcctgcag aggaagccac tctgactctt tcttgcactt tctctcaaga tcgtttgccg
     3061 gatttgcaat ggtcttgcaa ttcttgtatt tctttatggt ttcttctgtg agagagaaat
     3121 taacgaatcc aggttgcacc tgggagaata cataaccctg agtggagtat gattcctgga
     3181 tcaaagtgcg gtcacggaaa tattttcctt catcaaatag atccccgacg tcattgtcag
     3241 agaattccat ggacgcaagg aggtcttcct tgcgaaacac aggctgaggt tttgggtttt
     3301 tgcgaggtct ttcgcgaggg ttgagttctt cattgagcgc atcattgtca aactcaaggg
     3361 agtaacctcc gaagtagcga atgtctccct caattagttt gtacgttaca actaccacac
     3421 ggccgagttc tggtttggta ggggatctcc aacggatttc gtaacctgtt ccagttggat
     3481 ccaggtccgc gtctacgtaa ccctgagctt ttgcataccc gaggattttg aatttgtcct
     3541 gctcaaactt ggtttcgtcg aaaatgccgt cttcaatggt cccttcttct ttttgctcaa
     3601 gcactccaat gagatcttcc ggatcaaggt gacgggcccc aatgatgttg atcttgccaa
     3661 tgggaatgtt ttccccttcg tctataatgt atttaactgt gacttcgttg ttttcgtcca
     3721 gctgagagac acggtaccaa atctccgtga ggaaatagcc ttctgaacgg tacttttcct
     3781 tgatgcgggg gatcgcagac ttcaccttct gctctgcata gacttctccc tctttaaacc
     3841 ccacaagact tttgagatca gccgcatata gcttgtctgt gccgacgaaa tcgacttctc
     3901 gtactcgcgg caattcgctg aattcgaaaa tcagaatcac gccatcctgt tcgatgacac
     3961 cgcgcaggga tatttttccg aaatatcccg acttgaacag agcctttatg tctgcattga
     4021 gagttgcatc gtctactttt gtgcctcgct ccagagtgag caaccctgtg atttcatccg
     4081 gagagacgtt gcgcaaaccc tcatagcgaa tgtccttgat gaccttccct acgaactggg
     4141 tgcgatccgg cagaagaggg aagacaaaga caggaatcag aactgcgatc cagactgcct
     4201 tgcccttcag gcgggatacg atgttgttga ttcttttgct caatcccgat ttgacctgac
     4261 gggacccgga ggattccgcg tcccggccag aaaatatttg ggaagagatg cgtcattaca
     4321 attctaaccg gccatagaaa ggcaatctcg agcaaattcc agggctcgcg cgccgcccat
     4381 catccttgcc acctcttgca ctctatgctc cgattgtagt ctctgaatgc gagacacggt
     4441 tcgcccgttc tcctgcttct tggccacctt gaaatgatga tccgccctcg aggctacctg
     4501 ggccagatga gtgactacaa gaacctggca gtcgcgtgct atgtgtctga gtctttctgc
     4561 aagagtgtgc gctatttcac cacccactcc cgcatccact tcatcaaaca ccaccagcga
     4621 tacgggacgc gcctccatca tgacggattt gcacgcaagc ataatcctcg aaagttcccc
     4681 gccgcttgcg atcttacgga gtgggtgcgc gggttctcca ggatttggtt tgaagtaaaa
     4741 ctccacccga tcgaggccct tctccccgat gacgagcgta ccctcttctt ccccgctcat
     4801 ttcccgatgg atggaaatgc gaatttctgc gccaggcatt ccaagtgcag acagctctcg
     4861 cgccatacgc tcttccagcc caggaatgca ggatctgcga agcctggaga gtaattctgc
     4921 ggcttcgacc aatctgcccc aggaatccgc cagattttgc tccagtttct tttcattttc
     4981 ttctacgttc gcaagctcgt gaatctcttt gagaaaatca tccctgactc tggccagcga
     5041 atgcccatgc ttcttgatca agcgtctata ggcctgaatc ctttcctcca catcctccaa
     5101 cctctcggga gaaaattgca ggcgatccat ttgctgacga atcgcatcat gaaaattctc
     5161 aagcagtatg cacgcctcgt tcaaatcgga aagagaaccg gcgaactccg ggatgatttg
     5221 atcgtgacga tcgaggagtt gctgaatgcg agtgactccc gtaaggactg catgttcttc
     5281 tcctccaagg agcaaagagg cgacgtgtag atcctgcgcc accttccctc cgttctgcat
     5341 caaagccttt tcctgcgtga gcctctccag ttccccttct tccggggaaa gctgatcaat
     5401 ttccttcaca gagtgatgga ggtattccag cctgtagtct ctttcttctt tttgcatccg
     5461 cacgtttcgc agcttcttcg aaagaccctg ccactcgtgg tagaacgatg ccactcgctc
     5521 gcgcagttga gaagattcgc agtacgcatc cagagactcc agttgatgat ccggttccaa
     5581 tagtctctgc tggtcatgct gtccgtgcat ttcaatgaga gaagtagcac gtgcttttag
     5641 acctgccagg ttggtctgtg ttccgttgat gaagacgcgg ctccttccat ccgcggacac
     5701 ttcccttctc aatacgatct catcttcatc cttttcccgg aagcgaccct caatcaacgc
     5761 ccgagccgca cccgcgcgca ctacacccgc accgagtctg ccccccagga gtgcagagat
     5821 agattgaatc aaaagcgatt tgcccgcacc tgtttcccct gtgagtacgg acagcccatc
     5881 acccggatga aatctcattt catccatgat accgaagttt tcaatgcgta gttcagtcag
     5941 catggggaca ggctaatatg taaacatatg ttagccaatc aaaatttggt actcttgtgt
     6001 attttttcga gtcgagaggg agtcgcaaat tgatttgtag gggtgggtcc ccgcaaaaac
     6061 ctggaatgaa tggcagtttc cgatcgaatc ttgaaccggg catccctggg gctgggaatc
     6121 ctcaccctct gcgtgatgat cctgggtccg ctggtgcgag ccaatgatgc cggacttgcc
     6181 tgcccggact ggcctctctg ctacggccat gtgatccctc cttacgaatt tagagtctac
     6241 ct
//
LOCUS       DAORFF010000178         6218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6633_length_6218_cov_3.617556, whole genome shotgun sequence.
ACCESSION   DAORFF010000178 DAORFF010000000
VERSION     DAORFF010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6633_length_6218_cov_3.617556"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..294)
                     /locus_tag="PKK76_08620"
     CDS             complement(82..294)
                     /locus_tag="PKK76_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68577.1"
                     /translation="MAGTAVKSSRIRLSVSIAASSAVARSTDVVMSTCPFARMMEAFP
                     FFSTWMWRTMQRPRPGDGRLQAKNLE"
     gene            complement(535..1563)
                     /locus_tag="PKK76_08625"
     CDS             complement(535..1563)
                     /locus_tag="PKK76_08625"
                     /inference="COORDINATES: protein motif:HMM:NF015252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HNL68578.1"
                     /translation="MDKKKIMIAGAAVGVVIIGVIVYFLVAGNSGREKTVALKPENWD
                     APFSTSKEMIDEVFAMFDEEDMPATFDVFLKGLESGKINMVWKLWELRRQCPQGMDRY
                     NCNSRILAYLMRKYPGAGGEKLATLVKRYLEFEEVMSITRMSDDLTMRERYDIMRKKR
                     HEIFQPDEEALVFGMEEAKMDLADKSAAFFKETAGQSGNQRVKAYEEMRKKAMGRYYD
                     EFVKDEPSFDRYETEIALRDTDFSKVNSSEQAALTNSIRVRYFGEAGAARMAAVEKQL
                     AAEDQAEKNYEAAEAKFLKENPNLTGDAKEAALKKLRSQYMGEEEADAYARRKQYEDA
                     MKAMNVTP"
     gene            complement(1661..2569)
                     /locus_tag="PKK76_08630"
     CDS             complement(1661..2569)
                     /locus_tag="PKK76_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HNL68579.1"
                     /translation="MKKKLLLISAFLAMGISAPAFAVPPLQGMYPIVLSHGLFGWGSD
                     STGIIDIIGYWGGMDSYLRSQGAIVYAPTKSPTASNQTRAGELKTKTALWMAANGYTK
                     VHFIGHSQGGLDTRYTIANLGMASKTSTFTSLAGVHGGSPVADVVAGLPTSIKNALAT
                     IVNAFVPLVYGKTSANALAALSSLTKSGVAAFNAATPNASGVKYYSYSGHMYLLDPIQ
                     HPLMALTWPICSAVGLLNGYGADCDGLVPVSSAHWGTWKGEPSVGWFITGVDHLQISN
                     TLYSGQTWFDVSGFFLGVAKNAKANQ"
     gene            complement(2802..3683)
                     /locus_tag="PKK76_08635"
     CDS             complement(2802..3683)
                     /locus_tag="PKK76_08635"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNL68580.1"
                     /translation="MKLSFIAIVLFASAPLFADPAIVSAKSGLNIREKPDTKSKVLMT
                     VVTGGEVEVEEVLSKSENIQGKQANWMKVTYQDPSGGPSVSGFAFGGFLFREPGKHPV
                     ELREDFIKAQYGIVVTHQGTDDTHWSVSLKLQSGKVVTLWKNNGSSDGGSGGYYVADY
                     NPAAGFISFRQKDTPDYALVDYTTGKIHRLQYDVPSFNAAGTAYFESANGGMFGFHMD
                     VYEIGSGRVPRNVFAMHEEDLPGWDEFRAAWKDANTIECRYVHGGAKRGFLIKKTGGS
                     WVRLADCSVDALTGVCK"
     gene            complement(3756..4475)
                     /locus_tag="PKK76_08640"
     CDS             complement(3756..4475)
                     /locus_tag="PKK76_08640"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL68581.1"
                     /translation="MGDVLNHYEDHLADIYSWIYGDFHARLRANHDFFEMHGIVGPGN
                     AIDLGAGPGYQSIALCDLGFSVTAADFSAKLLDELHERAGQRNIQTLQADILEDVWFG
                     RSPSLIVCMGDTLTHLPSQDAVSSLLDSIYGELGPGGRMILTFRDLKGTPGEIRFIPV
                     RSSEDTIFTCVLTHEVEHVIVSDLIHQKSGSGWQQKVSSYRKLKIDPTVLDQQIRARG
                     FESLHFVSRDGLVEGIWRKPG"
     gene            complement(4475..5161)
                     /locus_tag="PKK76_08645"
     CDS             complement(4475..5161)
                     /locus_tag="PKK76_08645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNL68582.1"
                     /translation="MNCPKCSANMEIRAIDGVEIDLCTGCGGIFLDDGEFQSFTGMDP
                     ATGFVKLSKFVKVLAKLNERAVLDELTGVYTRKYFNEYMETLFASEKRGKITLIGIDV
                     DHFKTVNTEFGHDGGDAVLRTVAQRLKDCMRSSRDDSVFRIGGEEFCVVLLELSPDDS
                     YHSAENLRRLVQMDPIPMPNNGEKVITISMGVALARPTDTPESLYKRADELLYEAKNS
                     GRNRVVMEKA"
     gene            5334..>6218
                     /locus_tag="PKK76_08650"
     CDS             5334..>6218
                     /locus_tag="PKK76_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNL68583.1"
                     /translation="MLPARRSRAWSEFLGVFFSLSLVLITCAVQWAFWQYIQPTRWLL
                     FFPAMLFSALRWRAGGYTSTLGAATLVAIFFMPHTDTFPFIGPHDLLLSVIFTGGGLV
                     TSYTLNAARYIRMELKFHSEVLTNLAEGVMIVDTHNSKILFTNPRFDDLFGYSAGELL
                     GQHVSILNSKSNGAPRDRAQEIDDQLRKDGKWSGEVLNAKKDGTPFWCSVRITTFSPN
                     TADEVWIAVHDDITAAKAASEALALAERKFSDLAETIDEVFWMVNKDKTQMLYVSPAF
                     ERIFERTCASLYQNPLEWI"
BASE COUNT         1543 a         1633 c         1437 g         1605 t
ORIGIN      
        1 gaatatatac aatatagagg tgtatacgaa gacctccctc acgaggagat gtttcggtct
       61 cttcgcgaaa tctatcctca cctattctaa attttttgct tgcagccgcc cgtcaccggg
      121 acggggtctt tgcatggttc tccacatcca ggtagaaaag aaagggaacg cttccatcat
      181 tcgcgcgaaa ggacaggtag acatgaccac atccgtggat cttgccacag cactcgaagc
      241 cgcgatggaa acagacaatc tgattctgct ggattttaca gcagtgccgg ccattgactc
      301 atccgggatt ggagcggttg tgcgaatcgc tgaaaaaata aaaatgagcg gcaaaaagct
      361 ctgcatcttt ggtctttcta atcagctcaa gaaattgttc tgggttttga aactcgataa
      421 gaacctggcg atttttgaaa cagaatcgca ggcaatggcg ggacaaacta aaaaagcggc
      481 ctctgttcaa gaccgctttc tgcttctcga cgcggagatg ctcttaaggc gtcctcaagg
      541 agtcacattc attgccttca tggcgtcctc atactgcttt ctacgagcat acgcatctgc
      601 ctcttcctct cccatgtatt gcgaacgcag cttcttgagg gcggcttcct ttgcgtcccc
      661 ggtcagattt ggattttcct tcaagaactt tgcttccgca gcttcgtaat tcttttccgc
      721 ctgatcttcc gccgcgagtt gcttttccac tgcagccatg cgtgcggcac ctgcctctcc
      781 gaagtagcgc actcgaattg aattagttaa agcggcctgt tcggaagaat tcactttgct
      841 gaaatccgtg tctctcaatg caatttcagt ttcgtaacgg tcaaacgaag gctcatcttt
      901 cacaaactca tcgtagtagc gacccatcgc tttcttgcgc atctcttcgt atgctttgac
      961 cctttgattg ccggattgac cggcagtttc tttgaaaaag gcggcagact tgtctgccag
     1021 atccattttc gcttcttcca ttccaaaaac cagagcctct tcatctggct gaaaaatttc
     1081 gtggcgcttt ttgcgcatga tgtcgtatct ttcgcgcatg gtcagatcgt ctgacattcg
     1141 cgtgatgctc atcacttctt caaactcaag atatctcttc acaagggtgg ccagcttttc
     1201 tccgccagcg cccggatatt tacgcatgag atacgcgaga atcctgctgt tgcaattgta
     1261 acgatccatc ccctgaggac actgtctgcg cagttcccat agtttccaga ccatgttgat
     1321 tttcccggat tcgagaccct tcaggaaaac atcaaatgtg gctggcatgt cttcttcatc
     1381 gaacatggcg aatacctcat cgatcatttc tttcgaagta gaaaaagggg catcccagtt
     1441 ctctggtttc agtgcgacgg tcttttcgcg ccctgagttt ccagctacaa ggaaatatac
     1501 tataacacct ataataacca cgccgactgc ggcgcccgcg atcatgattt tctttttgtc
     1561 catatgcttt acgtttcaga gttgttggaa ggccgtgcaa gtaaaaaaga aaaagcggac
     1621 gcaagcgccc gctttcccaa aatgattctg gtctaaagtc ttactgattc gcttttgcgt
     1681 ttttcgcaac gcccaggaag aatccagata cgtcgaacca ggtttgtcca gaatacagag
     1741 tgtttgaaat ctgcaggtgg tctacacctg tgatgaacca gcctacagaa ggctcgccct
     1801 tccatgttcc ccaatgtgcg gaagaaactg gaaccagtcc gtcgcaatca gctccataac
     1861 cgttgagcag accaacagca gagcagattg gccatgtaag agccatcagt ggatgctgga
     1921 ttggatcaag cagatacatg tgtccgctgt aagagtagta ttttactccg gaagcgtttg
     1981 gtgtggcagc attgaatgca gcgacgcctg actttgtaag agagctgaga gctgccagag
     2041 cgtttgcgct ggtctttcca tacacaagtg gaacgaatgc gtttacgatg gttgcaagcg
     2101 cgttcttaat agaagttgga agaccggcaa ccacgtcagc tactggactg ccaccatgca
     2161 cgccagcaag tgaagtgaat gtagaagtct tggatgccat tcccaggttt gcgattgtgt
     2221 agcgagtatc aaggccgccc tgtgagtgac cgatgaagtg cacttttgtg tagccgtttg
     2281 cagccatcca gagtgcagtt ttggttttga gttctccagc gcgagtctgg ttagaagcag
     2341 tcgggctctt tgttggagca taaacgatgg cgccctgaga acggaggtaa gagtccattc
     2401 caccccagta accgatgata tcgatgatac ctgtagaatc acttccccat ccgaacagac
     2461 cgtgcgaaag tacgattgga tacattcctt gcagtggtgg cacagcgaaa gccggtgccg
     2521 agatgcccat cgcgaggaag gcagagatta gcagtaactt cttcttcatg acttaatttc
     2581 ccctctttta attgttctta ctgtaattag ccgccccacg gcattcacat tacgctccct
     2641 gtaatgcagg agtcgtgcca ggtcccttcc tggcctgtat ttgagatttt gcgtcgaatg
     2701 ggccgaatgg attagaattt tgaaatgcat tccaacattt cagattccaa aaccccggaa
     2761 aatccagagt tctatacact aaacaatgtt tagtcgttgg atcacttgca gacgccggtg
     2821 agcgcatcta cgctgcagtc ggccaggcga acccaggatc cacctgtctt cttgatcagg
     2881 aatccgcgct tcgcaccgcc gtgaacatag cggcactcta tggtgttggc atccttccac
     2941 gcagcgcgaa attcgtccca gccgggcaga tcctcttcat gcatcgcaaa tacattccgc
     3001 ggtacgcggc cgcttccgat ctcgtaaaca tccatatgaa aaccaaacat tccgccattc
     3061 gcgctttcga aatacgcggt gcccgccgca ttgaaagaag gaacgtcata ttgcaggcgg
     3121 tgaatctttc cggtcgtata atctacgagc gcataatcag gtgtatcttt ctggcgaaag
     3181 gagataaagc cggcggccgg attgtagtcg gctacgtagt atccgccaga gcctccatcc
     3241 gaactcccat tgttcttcca tagagtgacc acttttccac tctgcaactt taaggaaacg
     3301 ctccagtgtg tatcgtctgt tccctggtgc gtcactacaa tgccatattg agccttaatg
     3361 aaatcttcgc gcaactccac agggtgcttg cctggttcgc gaaacaggaa accaccaaac
     3421 gcaaaccctg aaacggaagg tccaccggaa ggatcctgat aagtcacctt catccagttt
     3481 gcctgctttc cctgtatgtt ttcagatttg gagagaactt cttccacttc gacttcgccg
     3541 cccgtaacca cagtcataag caccttagat ttagtgtctg gcttttcccg aatgtttaga
     3601 ccgctttttg cgcttacgat ggcggggtca gcaaagagag gagcagaggc aaacaatacg
     3661 atagcgatga aagatagctt caaaacaaaa cctccgaagg tagacgattc atgcaactgc
     3721 tgagttcatc ggctgcaacc gattttttga gaagtttaac caggtttgcg ccagattcct
     3781 tccacaagtc catccctgga cacaaaatgc agtgactcaa accctcgcgc acggatttgc
     3841 tggtccagga cagtcggatc gattttcagc tttctgtagg agcttacttt ttgctgccaa
     3901 ccgctccccg atttctggtg aatgagatca ctcacgatca catgttcgac ttcgtgcgta
     3961 agcacacatg tgaagattgt atcttcagaa ctccgcaccg ggataaagcg aatctcccct
     4021 ggagtcccct ttaaatcgcg aaaggtcaat atcatcctac cgcctggccc cagttcaccg
     4081 taaatggaat ctagaagaga gctgacggca tcctgtgaag gcaagtgagt gagagtgtcc
     4141 cccatgcaca caatcaaaga aggggagcga ccaaaccaga cgtcttcgag aatgtccgcc
     4201 tgcaatgtct ggatgtttct ctggcctgct cgctcatgaa gctcgtccag gagtttcgcg
     4261 ctgaagtccg ctgctgtcac tgaaaaacca agatcgcaca gtgcgatgga ttgataaccg
     4321 ggacctgccc cgagatctat cgcatttcca ggtccaacga ttccgtgcat ttcaaagaaa
     4381 tcatgattgg cgcgcaacct cgcatgaaag tctccgtaga tccagctata aatatccgcc
     4441 agatgatctt cgtagtgatt gagtacatct cccatcaagc tttttccatc accacgcgat
     4501 ttcgccccga gttctttgct tcatagagaa gttcatccgc acgtttataa agagactcgg
     4561 gagtatccgt tggccgcgca agagcaacgc ccattgaaat tgtgattacc ttttcaccgt
     4621 tattcggcat tggtatgggg tccatttgaa caagtctacg caagttttct gcagaatgat
     4681 agctatcatc cgggctcagt tcaagaagta caacgcagaa ttcttctcca ccaattctaa
     4741 agacactgtc atcgcgactt gagcgcatgc aatctttcaa tctctgggcg acagtgcgaa
     4801 gaactgcatc cccaccatca tgtccgaact cagtatttac tgttttgaaa tgatctacat
     4861 caattccgat cagagtgatc ttaccccttt tctcgctggc aaacaaagtc tccatgtatt
     4921 cattgaagta cttccgtgtg tacacgcctg taagttcatc gagaacagca cgttcgttta
     4981 actttgcaag aaccttcacg aactttgaga gcttcacaaa accggtggcc gggtccattc
     5041 cggtgaaact ctgaaactcc ccgtcatcca gaaagatccc tccgcagccg gtacaaagat
     5101 cgatttccac tccgtcgatg gcgcgtattt ccatattggc agaacacttt ggacaattca
     5161 taaaactctc ctgagcgagc gcacttacaa taaacgacta aagaaccact ggcatttcca
     5221 tcaagccaaa cagaaccttt tgcgcatact gtgaagcgtt cagtcagctt caattccagc
     5281 ccggggtcga ttaatttgct tgcggatcaa gcaggtgacg ttatgcaata tcaatgctgc
     5341 ctgcgcgccg ttctcgagcc tggtccgagt ttcttggcgt attcttcagc ctgagcctgg
     5401 ttctgataac atgtgcggtt cagtgggcgt tctggcagta catccagcca acgcgatggc
     5461 ttctcttctt tcctgccatg ctcttctccg ctctcaggtg gagggccggg ggctatactt
     5521 ctactcttgg cgcagcaacg ctcgttgcca ttttcttcat gccgcacacg gacacatttc
     5581 cattcattgg tccccatgat ctcttgctct ccgtgatttt cacaggagga ggtctggtta
     5641 catcctacac tctgaacgcc gcacgctaca taagaatgga gctcaagttt catagcgaag
     5701 tcttaaccaa tctggcggaa ggagtcatga ttgtggacac gcacaattct aagattcttt
     5761 tcaccaatcc ccgctttgat gacctattcg gatacagcgc aggagaactg ctcggccagc
     5821 acgttagcat cctcaactcg aaaagcaatg gtgccccgcg ggatcgagcc caggaaatag
     5881 acgatcaact gcgaaaagac ggcaaatgga gcggcgaggt actgaacgcg aagaaagacg
     5941 gaacgccctt ctggtgttct gttcgcatca ctacattttc accaaacacg gcagacgaag
     6001 tctggatcgc agtccacgat gacataacgg ccgcgaaggc tgcgtcagaa gctctggcgc
     6061 tggcggagag aaaattttcg gatctggcag agaccatcga tgaagtcttc tggatggtaa
     6121 acaaagacaa gactcaaatg ctatacgtca gccccgcatt cgaacgtatc ttcgagcgta
     6181 catgtgcaag tctatatcag aatccgttgg aatggata
//
LOCUS       DAORFF010000179         6216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6636_length_6216_cov_3.939133, whole genome shotgun sequence.
ACCESSION   DAORFF010000179 DAORFF010000000
VERSION     DAORFF010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6636_length_6216_cov_3.939133"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PKK76_08655"
     CDS             <1..259
                     /locus_tag="PKK76_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68584.1"
                     /translation="FVILKDAYGTDHSILIGNTDFKISKEMSIPLAGLTQAVHLEEKG
                     LELRWFLIVPASDKPASIQMDDLTVQTRDLIRIPPLAGSAQ"
     gene            complement(253..1098)
                     /locus_tag="PKK76_08660"
     CDS             complement(253..1098)
                     /locus_tag="PKK76_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNL68585.1"
                     /translation="MRILVSALVLLVLSCSSAFNKRQSLYVGDHEAFYRLKSASYPSE
                     EIRAQLPKQTAFPDLDEDSIARLLATLKYRKAGLFGDTNRRIFYNEQLPEIAHRFKES
                     QARLDETQRMVLIVTHDPDNSVLSHLERTTLVYWADEAGLNIVLGEIRQDIPHNDMLE
                     RQDWTSVLPVSMKHSYSDLRLIDSPHYQLKQINGSTHATWAVFPMDQLAQLPAIPPDN
                     RVSLSKTEPASTEPAKPAKDNQPPAKSDPQLTRRLQDLKQALDQGLITQEEYDQKRKK
                     ILDSY"
     gene            complement(1113..1457)
                     /locus_tag="PKK76_08665"
     CDS             complement(1113..1457)
                     /locus_tag="PKK76_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL68586.1"
                     /translation="MIEIVAKAPDRLAMRITDDLKMENAREFFDLFREHYTDSQVQVL
                     IDFGKIRFVDSSGIGILLRCAEEVKKRGGSFFICGLNKSMQSVFRLAGLHKIFELLEQ
                     EQANMRFPELQS"
     gene            complement(1454..1651)
                     /locus_tag="PKK76_08670"
     CDS             complement(1454..1651)
                     /locus_tag="PKK76_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68587.1"
                     /translation="MADVAVGGQYKLNRRTFLHKGIFVNQGVSVQVMEITDGPDKVIV
                     QFLDREGFPHILTGIGPEELE"
     gene            complement(1644..2429)
                     /locus_tag="PKK76_08675"
     CDS             complement(1644..2429)
                     /locus_tag="PKK76_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNL68588.1"
                     /translation="MDFERFKKINDERMNYREMEDASVVSSYRNLGCGDGYRLYLKVE
                     GDKVVDASYTTTGCGFGLVSLAMATQWITGKTIEQAGSIQASDIEGMFEFPDRRKNYP
                     ESAVEAVQKAVADFKNGTGIRPEDRVSRTTALEALARDGHLRGAKLNQIILEGENLEG
                     VDASGADFSNAFLQNVNFRGANLSGARFRGAFLNNANLEGANLSGADLRWAKLTGARL
                     EGVQVQGATYDIGTRVDANRIHLFKEMVQAGKDAYVEKEAGVG"
     gene            complement(2438..3985)
                     /gene="guaB"
                     /locus_tag="PKK76_08680"
     CDS             complement(2438..3985)
                     /gene="guaB"
                     /locus_tag="PKK76_08680"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004429403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNL68589.1"
                     /translation="MSDVHHPDVEFQPDGNSSDQIFEGGTGLTYRDYLVLPGYIDFHP
                     SDVHLETRVTRNISIHRPMISSPMDTVTEDRMAIAMALLGGLGVVHYNNTLERQVEIV
                     EKVKRYKNGFIEDPIVLGPHNTIRDLDMIKHKYNFSGIPITEDGTRNSRLIGIVTNRD
                     VDFEKDRTIRLSEVMTREVVTAQEGISLVDANKILKSSKKGKLPIINESGQLVALMCR
                     SDLKKNQEFPYASKDSQKRLMVGAAISTKMEAYDRLDALYRAGVDIVIIDSAQGNSNY
                     QIQTIKYIKKHYPSLEVIAGNVVTTDQCLNLIRAGADALRIGMGPGSICITQDVMAVG
                     RAQATAVYYTAKFARKYGVPVIADGGISTIGDLANALAIGASTTMMGSMFAGTQEAPG
                     DYFYENGVRLKKYRGMASLEAMEAGGDKRYFSEDQEVKVAQGVAGFVVDKGSMMQFVP
                     YLVQGLRQSFQDMGARSISELHEALYSGKLRFEKRSYSAQLQGSVHSLHSFTGPALGG
                     WEQNNRR"
     gene            complement(4081..5271)
                     /locus_tag="PKK76_08685"
     CDS             complement(4081..5271)
                     /locus_tag="PKK76_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNL68590.1"
                     /translation="MSGPLDGIRVIDLSMLLPGPLCSQHLADMGAEVIKIENPRVPDM
                     TRYFDAQYSGTEKPEASKDSAMFRLLNRNKKSVNLNVMRPEGREVLLRLLENADVLLE
                     GFRPGKMEELGIGYSNLKERFPRLVYCAISGYGDGGPLRDFAGHDGNYTARAAVLGLT
                     GAAGPVVPGIQVADIAGGTLIALSGILAALVSRAKTGLGQFVDVSMMDGAFSLIPLQA
                     AEYVATGKDPERGQMPLSGALPNYACYETKDGRFVMLGALEERFFKGFLRAAGKEDWI
                     DMLHRGDLKSLRARLQSLFASRTLAEWQPLFENTEACLAPVQTVSEAFQDPQLVARGM
                     VRRGRRGEDTWLEIGSPFRFSNTPVELDRLAPPGHGEHTREVLSAHGFSDLDIEEFKK
                     KRII"
     gene            complement(5268..5642)
                     /locus_tag="PKK76_08690"
     CDS             complement(5268..5642)
                     /locus_tag="PKK76_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNL68591.1"
                     /translation="MKPFGLLVFGLAITLNAMANILVKASALKKDDESAVGLIKGFLL
                     NPLLIAGLASFGLAFLAYRYVLSLGIPLSVAYPIMTTLGFAIVLLASRFFFQETLNGI
                     QWGGIGFLVVGLWMIASQMGQG"
     gene            complement(5655..>6216)
                     /locus_tag="PKK76_08695"
     CDS             complement(5655..>6216)
                     /locus_tag="PKK76_08695"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNL68592.1"
                     /translation="ERLRQVDLVLIDASIPENKFRVFPCGKFREGLEALSRASAVVLT
                     RADSKTAPEWKSRIQASFPRLRVFESTHEFAGLRPVLDRGPSASRPVTASAFCGIGNP
                     GAFFRMLESSGVKVQARCEFADHYAYAEKDLLMLRKRGGPWITTAKDAVRLRHFPDEY
                     LQAIWIADMRLRITVGSKDLIDLVFD"
BASE COUNT         1492 a         1677 c         1508 g         1539 t
ORIGIN      
        1 gtttgtaatt ctcaaagatg cgtatggcac cgatcattcg atcctgattg gaaatacgga
       61 tttcaaaatc agcaaagaaa tgtcgatccc tctcgcgggc ctgacccagg cagttcatct
      121 ggaagaaaaa ggcctggagc tcaggtggtt cttgattgtt ccagcttcag acaagccggc
      181 cagcattcag atggacgatc ttacagtgca gaccagggac ctgatccgga ttccgcctct
      241 cgcaggatcc gctcagtagc tatcgaggat cttctttctt ttctggtcgt actcttcctg
      301 agtgatcagc ccctgatcga gagcctgctt gagatcctgc agtctcctcg taagctgcgg
      361 atcgcttttc gccggaggct gattgtcttt ggcaggtttg gccggctcgg ttgacgcggg
      421 ctctgttttt gaaagggata ctcgattgtc aggcgggatc gcaggcaact gcgccagttg
      481 atccatgggg aacacggccc aggtagcatg agtggatccg ttgatctgct tgagctggta
      541 gtggggagag tcgatcaagc gcaggtcgct gtaggaatgc ttcatactga cgggaagaac
      601 actcgtccag tcctgtcttt ccagcatgtc attgtgagga atgtcctgac ggatttcgcc
      661 gagcacgatg ttcaaacctg cttcgtccgc ccagtaaacc agagtcgttc tttccagatg
      721 gctcagaacg ctgttgtcag gatcgtgagt cacgatcaaa accattctct gagtttcgtc
      781 tagcctggcc tgggattctt tgaatctgtg tgcaatctca gggagttgtt cattgtaaaa
      841 gattcttctg tttgtgtctc cgaatagacc cgccttgcga tatttgagag tggccaggag
      901 cctcgcaatg ctgtcctcat caaggtcagg gaaagcggtc tgtttcggaa gttgtgcgcg
      961 aatctcctct gatggatagg aggcagactt cagcctgtaa aaagcttcat gatcccctac
     1021 ataaagagac tgtcttttat tgaaagcaga tgagcaggat aggaccagaa ggaccagggc
     1081 agagacaaga attcgcatgg ttatagtatc ggctaggact ggagctcggg aaaccgcatg
     1141 tttgcctgtt cctgctccag gagttcaaag atcttgtgga gcccggccag gcgaaagaca
     1201 gattgcatag atttattcag gccacatatg aagaaggaac cgcccctttt cttcacttcc
     1261 tccgcacagc gcagaagaat gccaatgccg gaactgtcca caaagcgaat tttgccaaag
     1321 tcgattagaa cctgcacctg gctgtccgta taatgctcgc ggaacaggtc aaagaactcg
     1381 cgggcattct ccatcttgag atcatccgtg attctcatcg caagccgatc cggcgccttt
     1441 gcaacaatct ctatcattcc agctcttccg gaccaatgcc cgtcagtata tggggaaatc
     1501 cctcacggtc caaaaactgc acaataactt tatcaggtcc gtccgtaatt tccatcacct
     1561 ggacggacac tccctggttt acgaaaatgc ccttatgaag aaaagtgcgg cgattcagct
     1621 tatactggcc acccactgca acgtcagcca actccggcct ctttctctac gtaggcgtct
     1681 ttgcctgcct gaaccatttc tttgaacagg tggattctgt ttgcatccac tcgcgtacca
     1741 atatcgtaag ttgcgccctg aacttgaacg ccttcaaggc gcgctcccgt gagctttgcc
     1801 cagcgaaggt ccgctccgct gagattggcg ccctctagat tcgcattgtt caaaaaagct
     1861 ccgcgaaacc gcgcgccaga taagttcgct ccgcgaaagt ttacattctg taagaacgca
     1921 tttgaaaagt ccgcaccgga cgcatccaca ccttccaggt tttcgccttc cagtataatc
     1981 tgattgagct tcgcaccgcg aaggtgccca tcgcgtgcca gcgcctcaag agcagtcgtg
     2041 cgagagactc tatcttccgg acgaatgcct gtgccattct tgaaatcggc cactgccttt
     2101 tgcactgctt ccactgcgga ttctggataa ttcttgcgac gatctggaaa ctcgaacatt
     2161 ccttctatgt cggaagcctg aatggaacca gcctgctcta tggttttccc tgtgatccat
     2221 tgagtagcca tggccagaga tacaagacca aagccgcaac cagttgttgt gtaagaagca
     2281 tctacaactt tgtcaccttc tactttcaag tacagtcgat agccgtctcc acatcccaga
     2341 ttgcgatagg aagaaacgac agacgcgtct tccatctcgc gatagttcat gcgctcgtcg
     2401 tttatctttt tgaatcgttc gaaatccatg ggaccgccta tcttcgattg ttctgctccc
     2461 agccacccag agctggaccg gtgaaactat gaagactgtg cacagagccc tgcaattgag
     2521 cgctgtaaga tcttttctca aatctcagtt tgcctgagta aagtgcttca tgaagttcag
     2581 aaatggaacg cgcacccatg tcctggaagg actgtctgag cccctgaacc aggtagggaa
     2641 caaattgcat catagaccct ttgtctacga caaatcctgc cacaccctgt gcgactttca
     2701 cttcttgatc ttctgaaaaa taacgcttat ccccgcctgc ttccattgcc tcaagagaag
     2761 ccatgcctct gtactttttg agacgtacac cgttttcgta gaagtaatcg ccgggggctt
     2821 cctgggttcc tgcaaacata gagcccatca tggttgtgga tgcgccaatg gcgagtgcgt
     2881 tggcaaggtc tccaatggtt gagatgccgc cgtctgctat gaccggaaca ccatacttgc
     2941 gcgcgaattt cgcggtgtag taaacagcag tggcctgagc gcgacccaca gccattacat
     3001 cctgggtgat acaaatggat cccggtccca ttccaatgcg aagagcgtcc gcacctgcgc
     3061 gaatcagatt caaacactga tctgttgtta caacgttacc cgcaattact tccagggatg
     3121 gataatgttt cttaatgtat ttgatagtct ggatctggta atttgaattt ccttgcgccg
     3181 agtcaatgat cacaatgtcc acacccgcgc gatacaacgc gtcgagcctg tcgtacgctt
     3241 ccatcttggt tgagatagcc gcgccaacca tgagtctctt ctgagaatct ttggacgcgt
     3301 aaggaaactc ctggttcttc ttcaaatctg atctgcacat gagtgccacg agctgacccg
     3361 attcattgat gatgggaagt tttcccttct tggaagattt cagaatcttg tttgcatcta
     3421 cgagtgagat accttcttgc gctgtaacca cttctcgggt catgacctcg ctcaggcgaa
     3481 tggtccgatc tttttcaaag tcaacgtcac gattggttac aattccaatc aaacgtgagt
     3541 tccgcgtgcc gtcttctgtg attggaatac cggagaagtt gtatttgtgt ttgatcatgt
     3601 ccagatcgcg aatggtgttg tgaggcccaa gcacgatggg atcttcaatg aaaccgttct
     3661 tatatcgttt tactttctca actatttcca cctggcgttc cagagtgttg ttgtagtgaa
     3721 cgactcccag tcctcccaga agcgccatgg caatggccat gcgatcttcc gtgactgtgt
     3781 ccatgggtga ggaaatcatt ggacgatgaa tgctaatatt gcgcgttaca cgagtctcca
     3841 ggtgcacgtc gctggggtga aagtcaatgt aaccggggag caccagatag tcgcgatacg
     3901 ttaaccccgt tccaccctca aaaatctggt cggatgaatt tccatccggt tgaaattcaa
     3961 catccgggtg atgaacgtca gacatttcga ggatcgcgtc gtgtgccatg gggaaccttg
     4021 attatgacgc ataacctttc aagcaaattc tataaaacct gtacccaggc cgaaatcgcc
     4081 tcagatgatg cgctttttct tgaactcttc tatatctaag tcagaaaagc cgtgagcgga
     4141 aagcacttcc ctggtgtgtt cgccgtggcc cgggggcgcc agacgatcca gttccacggg
     4201 cgtatttgaa aaccgaaagg gagaaccgat ttcaagccag gtatcctccc ctctcctccc
     4261 acgtcgaacc atcccgcggg ccactagctg cggatcttga aatgcttctg agacagtctg
     4321 cacaggagca aggcaagctt ccgtattttc aaagagtggc tgccactccg cgagagtgcg
     4381 cgaagcaaag agagactgaa ggcgggcgcg aagagacttg aggtctccgc gatgaagcat
     4441 gtcgatccaa tcttcctttc cggccgcccg gagaaaccct ttgaagaatc gctcttctag
     4501 agctccgagc atcacgaatc gcccatcttt tgtttcatag catgcatagt tcggaagtgc
     4561 acctgaaaga ggcatctgcc ctctctctgg atccttaccc gtggcaacat actccgccgc
     4621 ttgaagtgga atcaaagaga acgcgccgtc catcatactg acatctacga actggcccag
     4681 gccagtcttc gcgcgcgaca cgagtgctgc aagaattcct gaaagcgcga tcaatgtccc
     4741 gcctgcaatg tctgctacct ggattccagg caccacgggg cctgctgctc cagtcaggcc
     4801 gagcactgcc gcacgcgctg tgtaatttcc atcgtgaccc gcaaagtccc gtagcggtcc
     4861 gccatctcca taccctgaaa tcgcgcaata cacaagcctc gggaaacgtt ctttgagatt
     4921 ggaatatcca attccaagtt cttccatctt gccaggccgg aagccttcga gcaaaacatc
     4981 cgcattctca agcaatctca acagaacttc acggccttct ggccgcataa cgttcaaatt
     5041 tacggatttc ttatttcgat tcagaaggcg aaacatcgcg ctgtccttcg atgcttccgg
     5101 tttttctgtt ccagaatatt gtgcgtcaaa gtagcgcgtc atgtccggga cacgcggatt
     5161 ttcaatcttg attacctccg ctcccatgtc cgccaggtgc tggctgcaca gcggaccggg
     5221 caaaagcatg gagagatcaa tgacacgaat gccgtctaac ggtccactca tccctgtccc
     5281 atctgcgaag ctatcatcca gagaccaacg acaagaaagc caatgcctcc ccattgaatc
     5341 ccgttgagtg tttcctggaa aaagaaacga gaggcgagaa gaacaatggc aaacccgaga
     5401 gtggtcatga ttggatatgc aacggacaga ggaatgccca gactgagcac ataacggtat
     5461 gccaggaaag cgaggccaaa agacgcaaga cccgcaatca aaagaggatt cagaagaaat
     5521 cctttgatta gccctactgc agattcatcg tccttcttca gcgcagatgc cttcaccaga
     5581 atattcgcca tcgcattgag agtgatggcc agtccaaata ctaagagtcc aaatggtttc
     5641 atttcgattc tgcctcaatc aaaaacaaga tcgatgagat ctttgctgcc cacggtgatg
     5701 cgaagcctca tatctgcaat ccatattgcc tgaagatatt catcggggaa atgccgaagc
     5761 cgcacagcat ccttcgctgt agtaatccag ggccctcctc gcttcctgag catcaacaga
     5821 tctttttctg cgtatgcata gtgatccgca aattcgcatc gcgcctggac ttttaccccg
     5881 gagctttcga gcattctaaa aaaagctccc gggtttccga tgccgcagaa ggcggacgcc
     5941 gtgacaggac gacttgcgga tgggccgcgg tccaggactg gacgtagacc tgcaaactca
     6001 tgggtggatt cgaaaactct caagcgagga aagctggcct ggattctgga cttccactcc
     6061 ggtgcggtct ttgagtccgc gcgtgtcaaa accactgcgc tcgcgcgcga aagcgcttcg
     6121 agcccttctc taaattttcc gcagggaaat acgcgaaact tgttctccgg aatgcttgca
     6181 tcgatcagca cgagatccac ctggcgcaac cgctcg
//
LOCUS       DAORFF010000180         6195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6673_length_6195_cov_3.669544, whole genome shotgun sequence.
ACCESSION   DAORFF010000180 DAORFF010000000
VERSION     DAORFF010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6673_length_6195_cov_3.669544"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..669
                     /locus_tag="PKK76_08700"
     CDS             106..669
                     /locus_tag="PKK76_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNL68593.1"
                     /translation="MDSTPLAAGIALFSFVYYLLIYTLWLKPTTVQSTVLGGVCGAIG
                     PMIGEAAIANRVGMPGIMLFLLLFLWQPPHFWALAIARREDYRKANFPLLPVLKGIPE
                     TLRSMIFYQWLLVVATIAVPLLGLAGWIFTVPSLFSSLVTLLLIYRMRKDASASSAMK
                     VFFASIAHNLVWHAAFSVDAAVGILRD"
     gene            974..1378
                     /gene="speD"
                     /locus_tag="PKK76_08705"
     CDS             974..1378
                     /gene="speD"
                     /locus_tag="PKK76_08705"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNL68594.1"
                     /translation="MEDPLGQHYIIELYECNHSVLDDLKSVRDTLMEAARIAGATIIG
                     EKFHRFSPQGVSGVVVIAESHLSIHTWPELGYAALDLFTCSHGMDIQAALDLLKRSFQ
                     PQDMDVHFMKRGVLKKSRSHAPELFDFVPAAV"
     gene            1393..2298
                     /locus_tag="PKK76_08710"
     CDS             1393..2298
                     /locus_tag="PKK76_08710"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HNL68595.1"
                     /translation="MITTKIHNTIQSYLNSKVRRMRGLAGDGLFAARAIKKDELVCMW
                     GGTIYTGEQFEEQPEAMKHYGLQVGENLYIAPANLDSVDDAEYFNHSCEPNCGFRGQA
                     ALVAMRDIRAGEELTFDYAMAETYDHPWKCECGSKHCRGTLRGTDHNLPELRDRYRGY
                     FSEWLEKKHADQLDDADYLHRFRATGAWGLDTGLDLHECNPEIIRSAEKIREFTIKLC
                     EKIEVKRFGEPVIVHFGEDERVAGYSLVQLIETSLISAHFANQTNRVYLDVFSCAYYN
                     PTEVAEFAKEFFQAKSVNVQTQLRH"
     gene            complement(2391..3641)
                     /locus_tag="PKK76_08715"
     CDS             complement(2391..3641)
                     /locus_tag="PKK76_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNL68596.1"
                     /translation="MELSKEQRKVVSANDRFLQVVAAAGSGKTRTMIALAEDSVTRGM
                     NPARILMLSFSRKACSEIRSRLSESIRSEVRVSTFHSLAFRSMMRRREMRGVRVLTDQ
                     AKFEFMRGLLCQAKTDGIPYSVLLANKRLFREQFPDLCMSVYRELAEYKRSNRLVEFD
                     DMVLDLNRELRLGHLRDLRKSFDLIIVDEFQDTDPGQLQFLQMMDSRRLVVVGDDYQS
                     IYGFRGSDPELFLKFQKHFPGARVLSLSENYRSQERIVRAGNHLIRSSRKQLRKKVRA
                     MRRGSTDCVALSVFRGEEAEVAALVNASGGFILCRSNYRRIVWERAGVEPDRLLTIHS
                     AKGLEFPIVYLDLCGGWSSARGTRVPDEEVRVAYVGLSRAENWLVVMHDESGTSAPER
                     AVFELFRNEVQRYSPVALQRAMAA"
     gene            3735..4082
                     /locus_tag="PKK76_08720"
     CDS             3735..4082
                     /locus_tag="PKK76_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNL68597.1"
                     /translation="MNNLALAVLDGNLTRDPETKTIKTGKITTTFTVAMNHEYGNKDG
                     NKSVSYIQVETWDKLAENCANFLKKGSRVTVSGSLREDRWKTEDGKARNRHKVVAQTV
                     RFDSPKKDQEEAA"
     gene            complement(4143..>6195)
                     /locus_tag="PKK76_08725"
     CDS             complement(4143..>6195)
                     /locus_tag="PKK76_08725"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNL68598.1"
                     /translation="VTNPLPIFDFLDGIFLGDGEEAILEIMGIIKSGKAAGKSRDEII
                     SDLSVVEGLLLPSLYTVDESSPGFPVYTGPMVKKRTYRADRFAALENILVPNIEITQD
                     RVVVEVNRGCGQGCRFCHAGFWKRPVRNAEVSTLVKTAGELIDRTGNDTITLHSLSIA
                     DYPWLEELVVEMANKYGPDGISISLPSLRVQVKTIPILEMTSGIRRSNVTFALEAASE
                     LMRERIHKKSSEDNLKFLVREIYSRGWDLVKIYFMMGLPDRDGHEVEDLIRSLNDFAD
                     LAHEMGPRKMVNVTVSLFVPKPFTTFQWEKQQGPEYFLDGLTRIKAGLKSKRVRIKGP
                     DPWMAYVEGLLSRSDHRVGQYILRAYKEGACFDSWDDRFQKDTWKKIIAEIPADLVSL
                     WMDQKPGGSRVPWDEIIDGALKEKLVRDFEKFESITPETMNPARVQELKPSDFPPELL
                     KPVTIPEEKFKTAGFLNLSFAKSGAFLYVSHLDTMNAMRKVLRRAKIPMTFSVGFNKH
                     ERLHFQGGTPTYFHSDAERLSAETYARFDLVAAEKRIRENLPMGLDLLELSFTDRKGF
                     SEAAWHSFRVDFFTESKFEPVRARLNDLPEEISFEKRDRKKKHGPRHANFMKKVKKRV
                     RTCVRAVEAHEAADGMAWIKFELESTERGGGISVTDLLSRVLDLPVETWNVDVRLTRL
                     S"
BASE COUNT         1568 a         1623 c         1495 g         1509 t
ORIGIN      
        1 gacccaatgc ccgcctgggt atcgatgaga aggtagtcaa aatcactagc aacctcctcg
       61 attccttcaa tgagattggt gcgttgagag gcggtcagtg aacagattga ttccactcca
      121 ctcgctgcgg gaattgctct tttctctttt gtatattacc tcttgattta cactctctgg
      181 ctcaagccaa cgacagtgca gagcacagtt ctaggaggag tgtgcggcgc gatcggtcca
      241 atgattgggg aagccgccat tgcaaatcgc gtcggaatgc ccggaattat gctgtttttg
      301 ctactttttt tgtggcagcc tccgcacttc tgggcgcttg cgattgcgag aagagaagac
      361 tatcgcaagg caaactttcc gctcctgcca gtgctcaaag ggattccaga aactcttcga
      421 agcatgatct tttaccagtg gcttctcgta gtagccacaa ttgctgttcc acttctcgga
      481 ctcgcaggct ggattttcac agttccatct ctcttctcct ccctggtgac cctgcttttg
      541 atttacagaa tgcgcaaaga cgcctcggca agctctgcca tgaaagtttt tttcgcttcg
      601 atcgctcaca acctcgtctg gcatgctgct ttctctgtgg atgcggctgt gggaattctc
      661 agagattgaa cactgcagta cgcgtgcctt atgtcggatt tcggcaaaaa aatcgccgtt
      721 tcacggctat tttcggggtt tcttgtctgg gacataatcc cgcgaagtca tttcatccga
      781 gtcgaggaag atttgcgcgg gtgaaattcg cgctgtcgca aaatctgaaa aatttttccc
      841 gaagattttt ttttaatttg cgtaaaaaat gacgataggt actcacgtgc aaacgtcaaa
      901 cgaatagttt gatcggggtt ttctaaatag ttgcaatcct acaagtatct agtaggagca
      961 gcgggaggtg aggttggagg atccgcttgg ccaacattat atcatcgagt tgtatgagtg
     1021 caatcactca gtactggatg atctcaagtc tgtcagagac actctgatgg aggcggcccg
     1081 gatagccggt gccactatca ttggcgagaa gttccatcgg tttagcccgc agggcgtatc
     1141 cggtgttgtc gtaatcgccg aatcccacct gagcatacat acctggcctg agcttggtta
     1201 tgctgctcta gatcttttca cctgtagcca tggcatggac attcaggcgg cactggattt
     1261 gctgaaacgc agcttccaac cgcaggatat ggacgttcat ttcatgaagc gcggtgtttt
     1321 gaaaaaatcc agaagccatg caccggaatt gtttgatttc gttccggcag cggtttgaag
     1381 gagggaagca ggttgatcac aactaagatc cacaacacaa tccaatccta cctcaactcg
     1441 aaggttcgca gaatgcgcgg ccttgcgggg gacggtctat ttgcagcgcg cgccatcaaa
     1501 aaagatgagc tcgtatgcat gtggggcgga acaatctaca cgggcgaaca gttcgaagaa
     1561 cagccagaag ccatgaaaca ctatggcctt caggtgggcg agaatctgta tatcgcgccg
     1621 gccaacctcg attccgtgga cgacgcggaa tatttcaacc acagctgtga accaaactgc
     1681 ggctttcgcg gtcaggcggc tcttgtggct atgcgggaca tccgtgcggg agaagagctc
     1741 acgttcgact acgccatggc ggaaacgtat gaccacccgt ggaagtgcga atgcggcagc
     1801 aaacattgcc gtggcaccct gcgtggaaca gaccacaacc tgccggagct tcgggaccgt
     1861 tatcgcggtt acttcagcga atggttggag aaaaagcatg cagaccagct ggacgatgca
     1921 gactacctgc accgcttccg ggcaacgggt gcatgggggc tcgacacagg tctcgacctg
     1981 catgaatgca atccggaaat catccgtagc gcagaaaaga tccgcgagtt tacaatcaaa
     2041 ctctgcgaaa agatcgaagt gaagcgcttt ggagaaccgg tcatcgtgca cttcggcgaa
     2101 gatgaacgag ttgccggcta ttctctggtg caattgatcg agacttcatt gatctccgct
     2161 cactttgcaa accagaccaa tcgcgtgtat ctggatgtat tcagctgcgc ttactacaac
     2221 ccgaccgagg ttgcagaatt cgcaaaagaa ttcttccagg caaaatccgt aaacgtccag
     2281 acgcagctca gacattgatc tgagctctgc tcctggcaag aaaccccggc caatggtcgg
     2341 ggtttttttt atgcctttgt tcaaagccgt atctggccta aaagctaccg tcaagcggcc
     2401 atcgctcttt gcagcgcgac gggcgaatac ctctgcactt catttcgaaa gagttcaaac
     2461 accgctcttt ctggagcgga tgttccagac tcgtcgtgca tcacaaccag ccagttttca
     2521 gcgcgactca aaccaacata ggcgaccctc acctcttcgt ccggcacacg agttcctcta
     2581 gcagaactcc aaccgccaca aagatccaga tatacaattg gaaactcaag acccttggca
     2641 gaatgaatcg taagcaatcg gtccggctcc actcccgccc tctcccagac aattcttctg
     2701 tagttgcttc tgcaaaggat gaagccgccg gatgcattca caagagctgc cacctccgct
     2761 tcttctcctc tgaatacgga aagggccacg caatctgtag atcctctgcg catggcgcgc
     2821 accttctttc tcaattgttt gcgcgatgac cggatcaaat gattgcctgc gcgcacgatt
     2881 ctttcctgac tcctgtaatt ctcgctcaaa gaaagaactc gcgctcccgg gaaatgcttc
     2941 tgaaatttta ggaataattc aggatcgctt cctcgaaagc cgtagatgct ttgataatcg
     3001 tctcccacta caacgagtct ccgcgaatcc atcatctgga gaaattgaag ctgcccggga
     3061 tctgtgtcct gaaattcgtc tacgatgatg agatcaaacg atttgcgcag atcgcgcaga
     3121 tgtcccagac gaagctcgcg gttcaagtcc agaaccatgt cgtcaaactc caccaggcga
     3181 ttggatcttt tgtattccgc aagctctcta taaacactca tgcaaagatc cggaaactgc
     3241 tctctaaata gcctcttatt ggcaagcagg accgagtagg gaattccgtc tgttttagcc
     3301 tggcagagga gcccgcgcat gaattcaaac ttcgcctgat ccgtcagaac tcgcacgccg
     3361 cgcatctctc tccttctcat catagaacga aaggcaagag aatgaaatgt ggaaacgcgc
     3421 acttcgcttc gtatggattc tgaaagcctg gagcgaattt cagagcatgc ctttctacta
     3481 aaactgagca tcaagatgcg cgccggattc atccctctgg taactgagtc ttccgccaga
     3541 gcgatcatgg tgcgagtctt gccgctgccc gcggccgcca ccacctggag gaaacgatca
     3601 ttggcactca ctactttccg ttgttctttc gatagttcca tcctatcact gggtaatcga
     3661 aaaagaaaat gatccgaaca atttgtgcgg aacgatcaaa agcacagtta cccttccctt
     3721 gcaaggagac acttatgaac aatctggcac ttgcagtgct tgatggaaac ctcaccaggg
     3781 atccggagac aaagacaatc aaaacgggaa aaatcaccac cacctttacg gtggcgatga
     3841 accacgaata tggaaacaaa gacggaaaca aatccgtctc ctacattcag gtggaaacct
     3901 gggacaagct cgcagaaaac tgcgccaact ttctaaagaa aggcagcaga gtgactgtga
     3961 gtggttcttt acgagaggac cgctggaaaa cagaagatgg caaagcgcgc aaccgccata
     4021 aggtggtagc acagaccgtc cgattcgaca gtccaaaaaa ggatcaagaa gaagccgctt
     4081 gagttagccg ggccaggcct ctgaaagacg atacgtcaca acgcctgcag gcgtgcgccg
     4141 tctcaggaga gcctggtcaa tctcacatct acgttccagg tttccactgg aagatcgagg
     4201 acccgcgaca ggagatctgt gacggaaatt ccgcctcctc tctccgtgga ctccagttca
     4261 aacttgatcc aggccattcc atctgctgct tcgtgcgctt ccacagcacg cacacaggtg
     4321 cgtactctct tcttaacctt cttcatgaaa ttcgcatgac gtggtccgtg cttcttcttg
     4381 cgatctctct tctcaaatga aatctcttcc ggcagatcat tcaatcgcgc tcgcaccggc
     4441 tcaaatttgg attctgtaaa gaaatctacg cggaaggaat gccaggctgc ctctgaaaat
     4501 cccttcctgt ctgtgaaaga caactcgagc agatccagac ccatgggcag gttttctcga
     4561 attctctttt ccgcagccac cagatcaaaa cgagcataag tttccgcaga aagcctttct
     4621 gcatctgaat ggaagtaagt tggcgttcca ccctgaaaat gaagcctttc gtgcttgttg
     4681 aacccaacgg agaaagtcat aggaatcttc gcacggcgaa gcacctttcg catggcattc
     4741 atcgtgtcca ggtgactgac gtagagaaac gcacccgatt tggcaaaaga cagattgaga
     4801 aagccagctg tcttgaactt ctcttctgga atggtgacag gtttgaggag ctccggagga
     4861 aaatcggagg gcttgagttc ctgcactcgc gcaggattca ttgtttccgg agtgatggac
     4921 tcaaattttt caaaatcgcg aaccagtttt tctttcagag ccccgtcaat gatctcatcc
     4981 cagggaactc ttgatccgcc tggcttctgg tccatccaca aagaaacaag atctgctgga
     5041 atttccgcga tgatcttctt ccaggtatct ttctgaaaac gatcgtccca ggaatcaaaa
     5101 caagcacctt ctttgtaagc acgcaaaatg tactgaccca ctcgatgatc ggatctggac
     5161 agaagtcctt ccacgtatgc catccaggga tctggtccct tgattcgaac tctctttgat
     5221 ttaagacctg ctttgattcg agtgagacca tcgagaaaat attcaggccc ctgttgtttc
     5281 tcccactgaa atgtggtgaa aggcttggga acaaataagg aaacagtgac gttgaccatt
     5341 ttgcgcgggc ccatttcatg agccaggtcc gcaaagtcgt tgagagaacg gatgagatct
     5401 tctacttcgt gtccgtcccg atcaggcaga cccatcataa agtagatctt caccagatcc
     5461 cagccgcgcg aatagatttc gcgtacgagg aatttcaaat tgtcttcgga tgatttcttg
     5521 tgaattcgct cgcgcataag ctcactggct gcttcgagcg cgaaggttac attggaacgc
     5581 cggatgccgg aagtcatttc caggatagga atggttttca cctgcacgcg aaggctggga
     5641 agagaaatag aaatcccatc tggcccgtac ttatttgcca tttctactac aagctcttcg
     5701 agccatggat agtccgcaat ggagagagaa tgaagagtga tcgtatcgtt tcctgtgcga
     5761 tcgatcaact cacctgctgt ttttaccaga gtggatactt ctgcatttcg caccggacgc
     5821 ttccagaatc cagcatggca gaatctgcaa ccctgcccgc atcctcgatt cacttccaca
     5881 accactctat cctgagtgat ttcaatgttt ggcacgagta tattttcgag agctgcaaat
     5941 cgatctgcgc gatatgtgcg tttctttacc attggacccg tgtaaacagg aaacccgggc
     6001 gagctttcgt ccacagtgta aagagacgga agcagaaggc cttctacaac ggaaagatct
     6061 gaaatgatct catcgcgcga tttgcccgca gccttgcccg acttgatgat gcccatgatt
     6121 tctagaattg cttcctcccc gtctccgaga aagatgccgt ccagaaagtc aaagataggc
     6181 agaggattgg tgact
//
LOCUS       DAORFF010000181         6102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6846_length_6102_cov_4.144369, whole genome shotgun sequence.
ACCESSION   DAORFF010000181 DAORFF010000000
VERSION     DAORFF010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6846_length_6102_cov_4.144369"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKK76_08730"
     CDS             <1..462
                     /locus_tag="PKK76_08730"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNL68599.1"
                     /translation="ATGSYGGIAGADAMCSQDAARPAAATYPIKAFLVGTTRRASLTA
                     SAGDGQLDWVLRSNQQYRRSDGTTVIGTTGSNALFTFPLAASFGTVGIGFRTGLNVNW
                     TTVGNDCLSWTDGSVGQTSEIGTANQTNNTAIGGGNITCSNPGYYLMCVEQ"
     gene            551..1192
                     /locus_tag="PKK76_08735"
     CDS             551..1192
                     /locus_tag="PKK76_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68600.1"
                     /translation="MFNKATTAVLSAALLLAGCGPSIITNSKAKFPVPVADNAPAFLF
                     PINLSHAGISGDSKTQGISVSAGIAAKFGKKVVSGQQLFDQVGNLSYELAEAISSQAK
                     AGTWTMTGPAATVASSLSTTMTGILSKLVSLNLIPAGYKFKYIIAVHSHGSAGMAPKT
                     VVVNSWGGIYDIESKEILDYIESSDTMVDDEKVALGNFPIVYNGIIQHLLDGK"
     gene            1248..1640
                     /locus_tag="PKK76_08740"
     CDS             1248..1640
                     /locus_tag="PKK76_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68601.1"
                     /translation="MIRILLTSLLVFSSFACGKKEPVADSELIGTWRGSVLIVDVEFI
                     FTKDSYQQRTFSFNNVMIGGTKGSMTVEGPVIRVHQTQEYRYDNDTRKGAWFDKEANY
                     TISYKISGNNISLHPSTSSETMELSKEQ"
     gene            1637..2341
                     /locus_tag="PKK76_08745"
     CDS             1637..2341
                     /locus_tag="PKK76_08745"
                     /inference="COORDINATES: protein motif:HMM:NF018784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HNL68602.1"
                     /translation="MKSVAPRIFACVLLFAVVSNCMTVLTLTNYKDRYQPVLYSGVRT
                     SLGYDPHAHNPLAGLVQTLMFIDLPFSAVLDTLILPATLPLAAFAQSKDHAFERQGNP
                     YVRLVLPPVLDLLLLQGNYSLEDGQNPFPPASRDAQQSDNSTYKLIHLPVKNLEAGVP
                     GCYLHCATREEASGLFSLGRSGGVSWRTLGLIRVKGVYKRERPYRNETFQYCLPDGGT
                     RGDDNVQSMAINRMWQ"
     gene            complement(2450..3397)
                     /locus_tag="PKK76_08750"
     CDS             complement(2450..3397)
                     /locus_tag="PKK76_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68603.1"
                     /translation="MTSAQEAQEALAAGEFARAKDIYGSLLEEYPEESDYICGFYTSA
                     YWDNRHSMILEAPPGKARGSALVEAWDQFEALANDKEFSGLSSFRTAMRSILGRAAEE
                     FRLSFQDSGLTHDSRTLAELGRCLIRIEDYANAAEILHFARKVRSTPEVLFLLGEALC
                     ASAAGDPETLAKGLSYYRDAFILEPDIIDPSLIASHPASDVFTRVYEEKDQNKELASV
                     WFPAKLMSLAQRLPLRRLLPEEVKEISKDLARLSPDLERVVEKYKERVRARICFFILV
                     LLQHHYLHDYNEEAIQELETRLAELDAHLYKEYRDYLHG"
     gene            complement(3398..3889)
                     /locus_tag="PKK76_08755"
     CDS             complement(3398..3889)
                     /locus_tag="PKK76_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNL68604.1"
                     /translation="MATEYSFDISSKVDRQELTNALDAVRKEVENRFDFKGSPVTIDL
                     EKDTLNLEAQDEMKLKQLIDVIQSKLVKRNISLKAFKFGPFESNVSGKFKCKTEIQSG
                     LNQEQCKKITKIIKESKLKVQSRIQEDQVRVTGKDRDELQAIIRMIKEANFDFDTSFS
                     NYR"
     gene            complement(3912..>6102)
                     /gene="alaS"
                     /locus_tag="PKK76_08760"
     CDS             complement(3912..>6102)
                     /gene="alaS"
                     /locus_tag="PKK76_08760"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNL68605.1"
                     /translation="NQFHQDTSGKLHPLPMTGIDTGAGLERITMLKLEENSVYDTDEM
                     KQIIRFIEGLTETLREDKKKVEYDPTNKHTAPSFRVLTDHTRATTFALSDGIYPDNTG
                     RGYVIRRIIRRGLLFAQEIGIKQPIMYRLVPEMVRIFGPFYPALKERAGEIELVIRKE
                     EERFLRTLEQGLARFKEYMAAFQAAKSPVFGGAEAFRLYDTYGFPLEMTVELAEKQGL
                     TTDMKGFEACMQEQRDRAHAHTAFKDWSFPADFKSPGESSFTGYERMQDSAKVQAILI
                     LRTKGESTDAESVPSIEAKDGPSQAILVFDHTPFYPEGGGQLGDTGQITSGQNVFTVL
                     DTKKKQGLILHIGEITHGKVSVGDSVELHVDSERRENLTHHHSATHLLNKSLRDTLGH
                     HVSQTGSLVSPDYLRFDFSHGEKIPADILRKVEEHVNAAIAKKAAVDARIMPIDQAKT
                     AGAVATFGEKYGTEVRVISMGEDGKLSRELCGGCHVPNTGNIQYFHIVKESSPGAGNR
                     RIEAVAGSAAARFFEEAIAKLTKAISAHNDQVHASTLSADEKKAFLIEQKATTEEKSR
                     LLGMGAAGVSPLTSLLEQDAVALEKAAREYSKLSRKTQGGATLSAEEVELEKLGDLEF
                     CARTFEGVSPENVKQLGDSLKEKHRKFCGLFGLKTDKGPVLIFMANRGAVEAGVDCGK
                     LIREASGLIGGGGGGKPEMAQAGGKDASGLESAVQRAKGLLKESLHS"
BASE COUNT         1334 a         1573 c         1522 g         1673 t
ORIGIN      
        1 gcgactggca gctacggagg cattgcaggt gcagacgcga tgtgctcgca ggacgcggcg
       61 cggccggcgg cagcaacata tccgatcaaa gcatttcttg ttggaacgac tcgccgtgca
      121 tctctcactg ccagtgcagg ggacggacaa ctcgactggg tcttgcgttc taatcagcaa
      181 tacagacgat cagacggtac gactgtcatt ggaactacag gatcgaatgc tctttttact
      241 ttccccctgg ctgcatcgtt tggaacggta gggattggct ttagaactgg attgaacgtc
      301 aactggacaa ccgtgggcaa tgattgtctg tcatggacgg atgggtcagt tgggcagaca
      361 tcggagattg gcactgccaa tcaaaccaac aacactgcca tcggaggtgg caacatcaca
      421 tgttccaatc caggctacta cctgatgtgt gtggagcagt aaattctgtt tatttccttg
      481 ccctgtaaaa gacccccggc atacctcggt tctggcccca ttcggggtca ttgatctcgg
      541 aggatattca atgttcaata aggcaactac ggcagtcctt tccgcagctc ttctgctggc
      601 tggatgtgga ccgtccatca tcacgaattc aaaagcgaag ttcccggttc cggtagcgga
      661 caatgcacct gcattcctgt tcccaatcaa cctttcgcac gctggaatct ctggtgattc
      721 taagactcaa ggaatcagcg taagcgctgg cattgcagct aaatttggaa agaaagtagt
      781 atccggtcag caactgtttg atcaggtagg aaacctctct tacgaactgg cagaagcgat
      841 ttcttctcag gcaaaagctg gaacatggac tatgactggt ccggctgcaa ctgtagcttc
      901 ttctctgtcc acaaccatga ctggaatcct cagcaagcta gttagcttga acctgattcc
      961 tgctggttac aagttcaagt acatcatcgc agttcactca catggatctg ctggtatggc
     1021 acctaaaact gtagtagtaa acagctgggg cggtatctat gatatcgaaa gcaaagagat
     1081 tctcgattac atcgaaagct ctgacacaat ggtagacgac gaaaaagttg ctctgggcaa
     1141 cttcccaatc gtatacaacg gtatcattca gcaccttctc gacggtaagt gattcaatca
     1201 ctccggggcc gcctgccatt ctgcgggcgg tctatctttt aaaaaccatg attcgcattc
     1261 tactaacttc tcttttagtt ttttcctctt tcgcctgcgg caaaaaggaa ccggtcgccg
     1321 attctgagct gatcggtaca tggcgcgggt cggttctcat cgtagacgtc gaattcatat
     1381 tcacgaaaga ctcctatcag cagagaacct tttcctttaa caacgtgatg attggcggaa
     1441 ccaaaggaag tatgactgtg gaaggccctg tgattcgcgt gcaccagacc caggagtatc
     1501 gctatgacaa cgatacgcgc aagggcgcct ggtttgacaa agaggcaaat tacactattt
     1561 cgtataagat ttccgggaac aatatctccc ttcatccgtc cacttcctca gaaaccatgg
     1621 agctttccaa agaacaatga agagtgttgc gccgcgcatt ttcgcgtgcg tcctcctgtt
     1681 tgcagtggtt tcgaattgca tgacggttct cactttgacc aactacaagg atcgctatca
     1741 gccggttctg tattcggggg taagaacaag tttggggtat gatcctcatg cgcacaatcc
     1801 cctggccggt ctggttcaga ctttgatgtt cattgatttg cctttcagtg ctgtcctgga
     1861 cactctgatt cttcctgcga ccttaccgtt agccgccttt gctcaaagca aggatcatgc
     1921 attcgaacga caggggaatc cctatgttcg tctggttctg cctccagtgc tggatttact
     1981 cttgcttcag ggtaactatt cgctcgagga tggacagaac ccctttcctc cagcttcgcg
     2041 ggacgcgcag cagtcggaca attccacgta caaattgatt caccttcccg tgaagaatct
     2101 ggaagccggc gttccaggct gctatttgca ttgcgccacg cgcgaggagg cctctggcct
     2161 tttttctcta gggcgcagcg gaggcgtttc gtggaggact ctcggcttga ttcgagtcaa
     2221 aggcgtctac aagagagagc gaccctatcg caatgaaacc tttcagtact gtttgccgga
     2281 cggcggaacg cgaggggatg acaacgtgca gtccatggcc atcaaccgca tgtggcaatg
     2341 acgctttccc tgcatgcaaa ggggattgca ttgcaagaaa tcattttggc agtctttact
     2401 acgtgaatcc ggacgcgggt aaggttcttt cagaatagcg cttccgcatt caaccgtgca
     2461 agtaatcgcg gtactctttg tacaggtgag cgtccagttc ggccaggcgc gtttccagtt
     2521 cctggattgc ttcttcattg tagtcgtgca ggtaatgatg ctgcaacaga accaggatga
     2581 agaagcagat acgtgcgcgc actctctcct tgtatttttc aaccacacgc tctaaatccg
     2641 ggctgagtct ggccaggtct ttgctgattt ctttgacttc ctccggcaaa agtcttcgca
     2701 gaggcaggcg ttgtgccaga gacatgagct ttgccggaaa ccacacggag gcaagttctt
     2761 tgttctgatc tttttcttcg tagactcgcg tgaatacgtc tgaagcagga tgagaggcga
     2821 tgagagatgg atctatgatg tccggttcta gaataaacgc atcgcgatag tagctgagcc
     2881 cttttgcaag agtctcggga tctccggctg cgctcgcaca tagggcctct cccagtagaa
     2941 agagaacttc aggtgtggat cgcacctttc gtgcgaaatg aagaatctcc gcagcgtttg
     3001 cataatcctc aatgcgaatg aggcatctgc ccaattccgc aagagtgcgc gaatcatggg
     3061 tcagtccact atcctggaac gataggcgga attcttctgc cgcgcgcccc agaatggacc
     3121 tcatcgcagt tcgaaagctg gaaagtcccg agaattcttt gtcgtttgca agtgcctcaa
     3181 actgatccca cgcttccacc agcgcgctgc cccgcgcctt gcctggaggc gcttccagga
     3241 tcatgctgtg gcgattgtcc cagtatgcgg atgtgtaaaa tccgcagatg tagtccgact
     3301 cctccgggta ttcttcgagc agagagccgt aaatgtcttt tgctcgcgca aattcgcccg
     3361 cggcgagagc ttcctgggct tcctgggcag atgtcatcta acggtagttg gaaaagctgg
     3421 tgtcaaaatc gaagtttgct tctttgatca tgcggatgat tgcctgcagc tcgtctctgt
     3481 cctttccggt tacacgaacc tggtcttcct ggattcgcga ttgaaccttt agtttagatt
     3541 ctttaataat tttggtgatc ttcttgcatt gttcctgatt gagtccgctt tgaatttccg
     3601 ttttgcattt aaactttccg cttacattgg attcaaacgg gccgaacttg aacgctttga
     3661 ggctgatgtt tctttttacg agcttggatt gaatgacatc aatgagttgc ttgagcttca
     3721 tttcatcctg ggcttccagg ttcaacgtgt ctttctcaag gtcgatcgta acaggggacc
     3781 ctttgaaatc gaatctgttt tcaacttctt tgcgcactgc atcgagtgcg ttggtcaatt
     3841 cctgacggtc aaccttggag ctgatatcga aagaatattc cgtggccatg cactctgatg
     3901 acctcctggc atcaggagtg caagctttct ttcaacagac ccttcgcacg ctgcacggcg
     3961 ctttcgaggc cggatgcgtc cttgccgcca gcctgcgcca tttctggttt gccgcccccg
     4021 ccgcctccta tgaggccgga ggcttcgcga atgagtttac cacagtcaac accggcttcg
     4081 accgcacccc ggtttgccat gaagatcagc accgggcctt tatccgtttt caacccaaac
     4141 aggccacaga atttgcgatg cttttctttc agcgaatctc cgagctgttt gacattttcg
     4201 ggtgaaacac cttcaaatgt gcgcgcgcag aattccaggt ctccgagctt ttctagctcg
     4261 acttcctccg cggaaagggt tgctcctccc tgggttttgc gcgagagctt ggagtattcg
     4321 cgcgccgctt tctcaagcgc cacagcatcc tgttccagaa gagaagtaag aggagacact
     4381 ccggccgctc ccattcccag gagcctcgat ttttcctctg tggttgcttt ttgctctatg
     4441 aggaacgcct tcttttcatc tgcactcaag gttgatgcat gcacctgatc gttgtgcgct
     4501 gatatggctt ttgtcagttt cgcaatggct tcctcaaaga atctggccgc agccgagccc
     4561 gccactgcct cgatccttct attgcccgca cccggactac tttcttttac gatgtggaag
     4621 tactgaatgt ttccggtatt gggaacgtga cacccaccgc atagctctcg cgaaagcttt
     4681 ccatcctctc ccatggaaat gacgcgcact tctgttccgt atttttctcc aaacgtggcg
     4741 actgctccgg ctgtctttgc ctggtcaatc ggcatgatgc gcgcatccac tgctgctttc
     4801 ttcgcgatgg ctgcgttgac atgttcttcc actttgcgca ggatatctgc gggaattttt
     4861 tccccgtgcg aaaaatcgaa tctcaggtag tcaggcgaaa ccagcgaacc agtttgcgaa
     4921 acatgatgcc cgagcgtatc gcgtaaagat ttgttcagca aatgagtggc actgtgatgg
     4981 tgagtgagat tctcacgcct ttccgaatct acatgcaact ccaccgaatc gcccacggat
     5041 actttgccgt gagtgatctc tccaatatgg agaatcaggc cttgcttctt ttttgtgtcg
     5101 agcacagtga atacattctg ccctgaggtg atttgtcccg tatccccgag ctgaccgccg
     5161 ccttccggat aaaaaggagt atgatcgaat accaggattg cctgagaagg tccgtccttg
     5221 gcttctatgc ttggaacaga ctccgcgtcc gtggattctc cctttgtgcg aagaatcaag
     5281 atagcctgca ccttcgcaga atcctgcatg cgctcataac ctgtgaatga actctctccc
     5341 ggagatttga aatccgctgg aaaagaccag tctttgaatg cagtgtgcgc atgcgctctg
     5401 tctctttgtt cttgcatgca ggcttcgaag cctttcatgt ccgtagtgag accttgtttt
     5461 tctgcaagct ccactgtcat ttcaagaggg aagccgtatg tatcatacaa tctgaacgct
     5521 tctgctccgc caaagacggg cgattttgct gcctgaaagg cggccatgta ttctttgaaa
     5581 cgagccagcc cctgttcgag tgtgcgaagg aatctttctt cttctttgcg aatcacaagt
     5641 tcgatttcac ctgcgcgttc cttgagtgca ggataaaaag gtccaaagat ccgaaccatc
     5701 tcaggtacga gtcgatacat gattggttgt ttgattccga tttcctgtgc gaacaggagt
     5761 ccacggcgaa tgattctgcg aatgacgtat ccccttcctg tgttatcggg atagattccg
     5821 tctgacagtg cgaaggtggt tgctcgcgtg tgatctgtga gtacgcgaaa ggaaggtgct
     5881 gtatgcttgt tggttggatc gtattcgact tttttcttat cttcgcgcag tgtttccgtg
     5941 agtccttcta tgaagcggat gatttgtttc atttcatccg tgtcgtagac tgagttttct
     6001 tccagtttga gcattgtgat tcgctcaagc ccggctccgg tgtctattcc agtcatgggg
     6061 agtggatgaa gttttccaga agtgtcctgg tgaaattgat tg
//
LOCUS       DAORFF010000182         6100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6850_length_6100_cov_3.569892, whole genome shotgun sequence.
ACCESSION   DAORFF010000182 DAORFF010000000
VERSION     DAORFF010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6850_length_6100_cov_3.569892"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /gene="aceE"
                     /locus_tag="PKK76_08765"
     CDS             complement(<1..686)
                     /gene="aceE"
                     /locus_tag="PKK76_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNL68606.1"
                     /translation="MAEPQLHSANTDIDPNESQEWREALDGVIAREGPDRAHYLIGQM
                     IAQAREAGIDIPYSATTEYINTVPVEKQPKYPGNAAMEIKIHNYIRWNAMAMVVRANK
                     STNVGGHIASFASAAALYDVGFSWFWRAPSENHGGDLVFYQGHSVPGIYARAHLLGRL
                     TDEQMDNFRQEVDGKGISSYPHPWLMPDFWQFPTVSMGLGPLQAIYAARFMRYMESRG
                     FIPAQDRKVWA"
     gene            complement(749..3904)
                     /locus_tag="PKK76_08770"
     CDS             complement(749..3904)
                     /locus_tag="PKK76_08770"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL68607.1"
                     /translation="MFFRREILLLLCFTSAPAFARMPVALENLRWSAREGFVESYRQG
                     LPSGTGTRSVDGFPISMFTLFPCPTDGFTHHFTISAPFELEPDDFSFQEPAGIFLRGL
                     GDNWELYVNGTLVHSEMNLNGGGQIAIRKTSMGKLIPFEKAILHKGTNTIVLHLAGTA
                     SIASAVNPAVPGLFFSKDYTMDRLSSLQHRYSERIDLALLAVYLFFGLYHLVLFLGRR
                     KDKYNLYFALFTISLAVYGFSRSYAIYDSIEDSSLVTRVENASVALLIPLLIVFLSDY
                     FYQKWIHPRLNWIICGFNFLAAALFCVVPFDFIAHVGWIWKTAAIPVILIYAPFIVVE
                     SVIKKARDAKILATALAIFVLVAIFDLVRDALKLFSMEPLFQYAFFGLVLSIAGILAH
                     RFVTVFNESDRLNQELAEKNETLRELDRVKDEFIGTISHELRTPLNGIVGLAEALTSG
                     RANDADIETGRTLGLIVDSGRRLSRLVGDLIELVRLKNRDLKVNPLPIRIADSVDVVL
                     SLIAPLVESRPITISSEIKPETPAVFADPDRLQQILFNLIWNAAKFTDKGRVTIRARP
                     SIKQPGFVTIEVEDTGIGIPAEKQRVIFEAFRQADSSIARPYSGAGLGLSITRYLVEL
                     HGGSISVTSTPGKGSIFCFTLPASTGLPLAAGSTATIEPIRYTTIRAPQTKKDRAKSS
                     ILAVDDEPINLEVLRTQLSLEGYDVTTAENGERALQMIEEDPPDLVVLDIMMPRISGY
                     QVCEEIRKHYSIHELPVLLLTARNQLTDLLRGFDSGANDYLTKPFEARELSARVRTLL
                     AVRHASQEKESHTALAKELAIARDIQRAVLPRTAPHVRGLTLATRFRPRDSVGGDFFD
                     FYAGEKTLDLIMADVSGHGVPAALIAAMIKIAFSHQRGVTDPAALLFGLNRALWGNLD
                     RHFVTAIALSIDLERRLLCLASAGHPPLLIHRAPNTSFYSARGRVIGLVEEAYYSKLE
                     VQLEPGDMLVVYTDGLTEARAPSGDLYGEDRLSAFIETHSHLSSDDLAEALEQDVVQF
                     AGETLDDDLALIVIQIDPKVN"
     gene            3960..4718
                     /locus_tag="PKK76_08775"
     CDS             3960..4718
                     /locus_tag="PKK76_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNL68608.1"
                     /translation="MTLFRALLILLLAVQSTYSQPPTASSIYGPVSREDYLLGRFRVD
                     QHPAFIKLSSTAIPTRGREQFLRKEAAEALGRLLGDLKRDHPQAEFWVVSSTRNYYDQ
                     KGIWESKWNGGTLVEGKALNKSIQDPAKRAQKILEFSSMPGTSRHHWGTDFDMNVLTN
                     VYYDSGNGKIVYDWFKKNASKYGFCQPYTAGRKKGYYEERWHWSYLPISRLLLADWKT
                     LFDHHTEKLKGFEGFAASGHLAPTYVESINPDCQ"
     gene            complement(4722..5792)
                     /locus_tag="PKK76_08780"
     CDS             complement(4722..5792)
                     /locus_tag="PKK76_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68609.1"
                     /translation="MRTRAVSVFALSAVFASALASFASLPARENLMSCSMDGRGTPNV
                     FGVCVEFPAESGRPGFYCGEDMTVNYIPCALRNRVGSCEMQSERGPFLVHYYSPQRNG
                     SGLPEFCAQRGKYHAPNSGYTLTLGPSPRKQPDPGKLLEETKEVSSWKESAGSPKEDG
                     LPDKPGGDKSAIRSCSPDAKGSHTGAGACVEFSGSVAEDYCAIGGKVSESRCDPSGRI
                     GTCRIEKPGTVIFVHHYPPALKENLQPTCEKFGGTLYAPGEDFINDMRPVEKRLERCN
                     DLIDLLRKLYEGAPASPQESSCSEYGKMIQLRLTEAPGLDAEQRSAVTMIGRTLCTSK
                     QNGTMSFAAFEAEFRKKRACPK"
     gene            complement(5819..>6100)
                     /locus_tag="PKK76_08785"
     CDS             complement(5819..>6100)
                     /locus_tag="PKK76_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68610.1"
                     /translation="DLRPLRFDLMRSEPGSASPLYDPSVCALVFSGYWVDVPGLHNER
                     FPPFSRKEMEIEDWIDQAYLWKWEELPPDVLKHNKALSEYIAKHLKSPP"
BASE COUNT         1473 a         1539 c         1577 g         1511 t
ORIGIN      
        1 gcccatacct ttctatcctg tgctggaatg aatccgcgac tttccatata gcgcatgaat
       61 cgcgcagcat agattgcctg cagaggaccg agccccatgg atacggttgg gaattgccag
      121 aagtcaggca tcagccatgg atgcggataa gacgagattc cttttccgtc cacttcctgg
      181 cggaagttgt ccatttgttc gtccgtcagg cgaccaagta aatgagctcg tgcataaatt
      241 cctggtacag agtgcccttg atagaagacg agatctccgc cgtggttttc agagggagcg
      301 cgccagaacc aggaaaaccc aacatcgtag agagctgctg cggatgcgaa agaagcaatg
      361 tgtccgccaa cgttagtcga cttattggcg cgcacaacca tggccattgc gttccagcga
      421 atgtagttgt gaatcttgat ttccatagca gcgttgccgg gatacttggg ctgcttttct
      481 acgggcacgg tgttgatgta ttccgtagtc gcgctgtaag gtatgtcgat tccggcttcg
      541 cgcgcctgtg cgatcatctg gccaatgaga tagtgagccc tgtctggacc ttctcttgca
      601 atgacaccgt ccagtgcttc tcgccactct tgcgattcgt ttggatctat atctgtattt
      661 gctgaatgca gctggggctc tgccatttat gcctcctccc cggaacaacg cgtggcgcgt
      721 cagactgtgg gagcctgcct ggcggacgtc aattgacttt tggatcgatt tgaattacga
      781 tgagggccag atcgtcgtcc agtgtttccc ccgcaaactg aaccacgtcc tgttccaggg
      841 cttccgcaag gtcgtcgctg gaaaggtggc tgtgggtttc aatgaaagcg ctgaggcgat
      901 cttctccata aagatcgcca gacggagcac gggcttctgt caggccatct gtatatacga
      961 ccagcatgtc tccaggctca agctggactt ctaacttgct gtaataggcc tcctcaacca
     1021 gaccgatgac tcttcctctg gcggaataga aactggtgtt aggagccctg tggatcagta
     1081 gaggtggatg accggcagac gccagacaaa gcagccttct ctccaggtca atgctcagag
     1141 caatggcagt tacaaaatga cgatccagat ttccccaaag cgcgcgattc aatccaaaca
     1201 aaagggccgc cggatctgtg actcctctct ggtgtgagaa cgcaatcttg atcatggcag
     1261 caatgagcgc cgctggaact ccatgtccgg acacatctgc catgatgaga tcaagagtct
     1321 tttcacctgc gtagaaatca aaaaagtctc cgcctacact gtctctgggg cggaagcgag
     1381 tggccagtgt aagaccacgc acgtgaggcg ccgttcgcgg gagcactgcg cgctgaatat
     1441 cgcgagcaat ggccagttct tttgcaagcg cagtgtgtga ttctttttcc tgggaagcat
     1501 gcctcacggc aagtagagtg cgcactctag cactcagttc gcgcgcctca aaaggttttg
     1561 tcaggtagtc gtttgcgccc gagtcaaaac cgcgaagcaa gtccgtcagc tgatttcgtg
     1621 cggtcaaaag aagtacagga agctcatgga tcgagtaatg tttgcgaatc tcttcgcaca
     1681 cttgatatcc tgaaatacgc ggcatcatga tatccagaac caccaggtcc ggcggatctt
     1741 cttctatcat ttgaagagcc cgctcgccat tttccgcggt ggttacatca tatccttcga
     1801 ggctcagctg cgtgcgaaga acttcgagat tgataggttc atcatctacg gccagaatgc
     1861 ttgatttcgc gcggtctttc tttgtctgag gagctcgaat ggtcgtataa cggattggtt
     1921 caatggtggc tgtgctaccc gctgcgagtg gcaatcctgt gctggccgga agagtgaaac
     1981 agaagatgga tcctttccct ggtgtagatg ttacggagat agaaccaccg tgcaattcca
     2041 ccagatagcg agtgatgctc agacccagac ctgctcccga atagggacgc gcaatgctgg
     2101 aatctgcctg gcgaaacgct tcgaaaatta cgcgctgttt ttctgcggga atcccaattc
     2161 ccgtgtcttc cacttcgatg gttacaaaac ctggttgttt gatgctcggt ctggcgcgaa
     2221 tcgtcactct ccctttgtcc gtgaatttcg ccgcgttcca gatcaggtta aaaagaattt
     2281 gttgcaggcg atctggatcc gcgaatactg cgggcgtttc cggtttgatt tcagatgaaa
     2341 ttgtgattgg tctgctttct acgagaggtg ctatgagcga cagcaccacg tctacagaat
     2401 ccgcgatgcg aatcggcaag gggttcacct tcagatcgcg gtttttcagg cgtacgagtt
     2461 caatgaggtc tcccaccagc cgagaaagtc tgcgaccgct gtccacgatc aatccaagag
     2521 tacgacctgt ttctatatca gcatcattgg ctctgccaga agtgagcgcc tccgcaagac
     2581 ctacaatgcc gtttaacggt gtgcgaagtt cgtgagaaat cgttccaatg aattcatctt
     2641 ttacccgatc cagttctctc agcgtttcgt ttttttctgc cagctcctgg ttcagtcgat
     2701 ctgattcatt gaatacggtg acgaatctat gagcgagaat tcctgcaatg gatagaacca
     2761 gaccaaagaa tgcgtattgg aaaagaggct ccatagagaa gagcttcagc gcgtctctga
     2821 ccagatcgaa tatggccacg agcacaaaga ttgccagcgc agtggccagg atttttgcat
     2881 ccctggcttt cttgatcacg gattctacga caatgaatgg cgcgtagatg agaatgactg
     2941 gaatggcagc tgttttccag atccatccta cgtgtgcgat gaaatcaaat gggactacgc
     3001 agaacaaggc ggctgcaaga aaattgaatc cgcaaatgat ccaattgagt ctggggtgaa
     3061 tccatttctg gtagaagtaa tcgcttaaaa aaacaatcaa aagagggatg agaagggcca
     3121 cgcttgcatt ttcgacgcga gttacgagag aggaatcttc tatagaatcg taaatggcgt
     3181 aggagcgaga gaatccgtac actgcaaggc tgattgtaaa aagagcgaag tacagattgt
     3241 acttatcctt tcttctaccc aggaaaagaa ccagatgata caatccgaag aatagataca
     3301 ctgcaaggag agccagatcg attcgttccg aataacggtg ctgcaaagag gacaggcggt
     3361 ccatcgtgta atctttgcta aagaacaaac caggaacggc gggattgacc gcggatgcga
     3421 tggacgccgt tcctgcgaga tggagcacaa tcgtatttgt tcctttgtgc aggattgctt
     3481 tttcgaatgg gatgagtttg cccatcgacg tttttcgaat ggcgatttgt ccgcccccat
     3541 tcagattcat ctccgagtgc accagggttc cgttcacgta gagttcccag ttgtctccga
     3601 gcccgcgcag aaagatcccg gcaggttctt gaaacgagaa gtcgtctggc tctagctcaa
     3661 agggggcgga aattgtgaaa tgatgtgtga atccgtccgt tggacaggga aaaagagtga
     3721 acatgctgat cgggaagccg tctactgaac gtgtccccgt tccagacggt agaccttgcc
     3781 tgtatgattc cacaaatcct tctcgtgcgg accagcgaag gttttcgagt gcaaccggca
     3841 tgcgcgcgaa agctggagcg gatgtgaagc aaagcagaag gaggatttcg cggcgaaaga
     3901 acatcgaaga tgtgaggatt tacgaatatg cttcaattta gacgagaaaa aaattgccca
     3961 tgacattgtt tcgcgctctg ctgattctcc ttctggcagt acaatcgaca tactcccaac
     4021 cgcccaccgc ctcatcgatc tacggaccag tgtcacgaga agactatctg ctcggtcgct
     4081 ttcgcgtgga ccagcatcct gcattcatca aactttcgtc tacggccatt cccactcgag
     4141 gtcgcgaaca atttcttaga aaagaagctg cagaagcgct cggtcgcctt cttggcgatt
     4201 tgaaacgcga tcatccccag gctgaattct gggttgtgag ctctacccgg aactattacg
     4261 atcaaaaggg aatctgggaa agcaaatgga acggagggac tctggtagaa ggaaaagcac
     4321 tcaataagtc cattcaggat cctgcgaaac gcgcccagaa gattctggag ttttcatcca
     4381 tgcccggaac atctcgccat cactggggaa ctgattttga catgaacgtt ctcaccaatg
     4441 tttactatga ttctggaaac ggaaagatcg tctatgactg gttcaagaag aacgcgtcta
     4501 aatacggatt ctgtcaacca tacactgcag gaaggaagaa aggatactac gaggaaagat
     4561 ggcactggtc gtatcttcct atttcccgcc tccttcttgc agactggaag acgttattcg
     4621 accatcatac tgaaaaacta aaaggtttcg aaggctttgc tgcttccggt cacctcgcgc
     4681 ccacatacgt ggagtccatc aatccggatt gtcaatagga gtcatttcgg gcaggccctt
     4741 ttctttctaa attcagcttc gaaagccgca aaggacatgg ttccattttg tttggaggta
     4801 cacagtgtgc ggcctatcat ggtgactgcg cttcgctgct ctgcgtcgag gcccggcgct
     4861 tctgtcagac gcaattgaat catctttccg tattcgctgc agctcgattc ctgcggtgaa
     4921 gcaggtgctc cttcgtagag tttgcgcaat aaatcgatca aatcgttgca acgttccagt
     4981 cttttttcca cggggcgcat gtcgtttatg aaatcttctc ccggtgcata cagagtccct
     5041 ccaaactttt cgcacgttgg ttgcaggttt tctttgagag cggggggata gtgatgaaca
     5101 aaaatgacag tgcccggttt ttctattctg cacgtgccga ttctgccgga gggatcgcag
     5161 cgcgattctg acacttttcc tccaatcgcg cagtagtctt ctgcaacact gcctgagaat
     5221 tctacgcacg caccggctcc cgtgtgactt cccttcgcat ccggtgaaca gcttctgatg
     5281 gcactcttgt cccctcccgg cttatccggg agtccgtctt cttttggaga tccggcgctt
     5341 tctttccagg aggatacttc ctttgtttcc tcaagcaact tgccaggatc aggctgcttt
     5401 cttggagagg ggcccagtgt aagcgtataa ccagagttag gtgcgtggta ttttcctctc
     5461 tgcgcgcaaa actcaggaag cccggatccg ttccgctgcg gagaatagta atgaaccaga
     5521 aaagggcccc tttctgattg catttcgcag ctccccactc gattgcgaag tgcgcacgga
     5581 atgtaattca cagtcatgtc ttccccgcag tagaacccgg gacgccctga ttctgcagga
     5641 aattctacgc acactccgaa cacgttcgga gtgcccctcc catccatgga acagctcatg
     5701 agattttcgc gtgcagggag ggatgcaaaa gaagcgagtg cggacgcgaa cacagccgaa
     5761 agtgcaaaga cgctgactgc gcgggtacgc acgtgccgat tgcctttcag ctcatggatc
     5821 acggcggact cttcagatgt tttgcaatgt attctgaaag cgctttgttg tgcttaagaa
     5881 cgtcaggcgg caattcctcc catttccaca aataagcctg atcgatccag tcttcgattt
     5941 ccatttcttt acgcgaaaac ggcggaaatc tctcattgtg gaggccggga acgtctaccc
     6001 agtatccact gaacacaagt gcacatacgg aaggatcata gagcggggag gcagatcctg
     6061 gttcgctcct catcaaatca aaacgaagtg gtcgcagatc
//
LOCUS       DAORFF010000183         6087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6874_length_6087_cov_3.779178, whole genome shotgun sequence.
ACCESSION   DAORFF010000183 DAORFF010000000
VERSION     DAORFF010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6874_length_6087_cov_3.779178"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="PKK76_08790"
     CDS             <1..992
                     /locus_tag="PKK76_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNL68611.1"
                     /translation="VSSLAVGATLVLFDGNPFHPTPGVLFDLAQKEGITVFGTSAKYL
                     SALEAAGVVPQKEFDLSRLRTILSTGSPLSRESYRYVYDQIKADVQLSSISGGTDLNG
                     CFALGNPVLPVYEGELQSRGLGMAVEVWDDSGKSVKEQKGELVCIRAFPSMPLFFWND
                     PDGSRYHKAYFDHFPGIWRHGDFAMITEHNGMIIYGRSDATLNPGGVRIGTADLYRIV
                     ESFGEIEDSVVVGQEWQNDQRVILFVKMKQGSLTEDLKKQISQKIRDSVSPRHVPAYI
                     FQVPDIPYTRNLKKVELAVKRVIQDEPVTNRDALVNPECLEFYKNIPELRSQS"
     gene            989..1981
                     /gene="hemB"
                     /locus_tag="PKK76_08795"
     CDS             989..1981
                     /gene="hemB"
                     /locus_tag="PKK76_08795"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNL68612.1"
                     /translation="MNVYDLPIRPRRNRRTESIRSIVREHSVSSTDLIQPMFVCEGNQ
                     KSEAIVSMPGIFRLSTDLIVKECVALFELGVPGVALFPAISDSKKDKKATESLNDDGL
                     LQTTVRAIKAAVPGITVITDVAMDPYSSDGHDGFVNEQGEIVNDVTLDILANMAVSQA
                     RAGADMVAPSDMMDGRVGVIREALDDHGFTNVGILSYSAKYASSYYGPFRDALSSAPK
                     SGDKKTYQMDPANVREAIYEVELDIAEGADMVMVKPGLPYLDVLRAVSEISNVPVAVY
                     NVSGEYAMLRAAAEKGWLDYQKAVLEALLSFKRAGADMILTYHAKEAAEWLKSK"
     gene            1963..3318
                     /locus_tag="PKK76_08800"
     CDS             1963..3318
                     /locus_tag="PKK76_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HNL68613.1"
                     /translation="MAQIKMNADVIVIGGGLSGLNAARLIHRAGKKVLVLEARDRVGG
                     RTLSKKFGNSVIDLGAQWVGPTQDRILRLAEEQGLRTFPQHTGGRQILALGDKVSTYT
                     GTIPSLPVHSLIELHLRITKIEKECRRVPLEAPWTFPDAEKWDAMTVESWKQNHVYTR
                     GARASLDAAVHAIFACEPSEISFLYFMHYLHSGGGLMRLADVKNGAQQDRIFGGTQQI
                     SSRMAADLGRRVILSSPVLRVEQTSRGVTVYTRKTKYSARRIIIALSPTLCSKIAFSP
                     ALPVARDQLHHRMPMGSVIKTFALYDRPFWRERGYSGEVVSDGTCVRLAFDASIPGSM
                     HGLLVGFISGENAREWSDRTPREREAAVVSDFVRFFGEEASDHRGYLEQNWIAEEFSR
                     GCYAGLMPPSVMTSFGNALRDPCGLIHWAGTETARHWNGYMEGALEAGERAAAEVLEA
                     R"
     gene            complement(3475..3939)
                     /locus_tag="PKK76_08805"
     CDS             complement(3475..3939)
                     /locus_tag="PKK76_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68614.1"
                     /translation="MMYREPDFYERISALNKVKLQRLISWLSEKELVALMNSADLELE
                     DAVLDNTSHAGRIVVLENLGFSEEDFLKRASVRKKISDLYAEQILNMETPPDKSQRAA
                     IVDGIVANLNEEERAAYNNRFKEKVVEIGEALRHKLSGYLDLLEKGASYKHM"
     gene            complement(3936..4709)
                     /locus_tag="PKK76_08810"
     CDS             complement(3936..4709)
                     /locus_tag="PKK76_08810"
                     /inference="COORDINATES: protein motif:HMM:NF021371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HNL68615.1"
                     /translation="MEASSQKQANTDGKIFLTADWRHLLLVNYEVNPDILAPHVPRGV
                     ELDLFGGKCFVSLVGFMFLRTRLLGKLPIPFHTNFEEINLRFYVKRKEGEETRRGVCF
                     LKEIVPRYAIALVARKAYGERYVSHPMSHILDIKNSALAVNGRVVYSWLDEKRHSIGA
                     KCTGNPSYPAAGSQEHFIIEHYFGYVKSPAGQTVEYRVEHPEWRVLSCKDPRVDVDVA
                     RPYGPEFVDPLSKTPASVFVAEGSPVTVRQGRILPEAAG"
     gene            complement(4718..5113)
                     /locus_tag="PKK76_08815"
     CDS             complement(4718..5113)
                     /locus_tag="PKK76_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNL68616.1"
                     /translation="MIEVEEGKTLSLDFEKTRKAADAGAVLPVIVQDAKTKDVLILGY
                     VNDAALKQSIETGFVTFFSTSRKEIWVKGDTSGNRLRLKEIRVNCEQNSLLFLAELEG
                     QGACHTKEKDKYRYSCYYRRLEQGSLKFV"
     gene            5195..>6087
                     /locus_tag="PKK76_08820"
     CDS             5195..>6087
                     /locus_tag="PKK76_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68617.1"
                     /translation="MQELLLKRHSVFRGDVTLPGSKSIANRALLLSSLAQGKTILRGL
                     PDADDVTVFRRNLPNLGIQATEMGDSLEIVGAGGSFPVQKADIFVENAGTAMRPLAAA
                     LCAGKGDFVIDGNEQMRRRPIGDLVRGLAELGVDITAAQATVQGKLEMVPPVTIHAKG
                     IAGGRTRLSGNVSSQFISAVLLAAPLASKPLTLEVPGDPVSKPYIDMTISMMKQFGAG
                     VRRERYSLFEIAPQRYISPGEFTIEGDASAATYFLSLGALSGPVTVRTIYSDSVQGDV
                     RFADVLSRMGAVVTRLDDSITV"
BASE COUNT         1440 a         1455 c         1610 g         1582 t
ORIGIN      
        1 tggtgagttc tcttgctgtg ggggccactc tcgtactttt tgacggcaat ccctttcacc
       61 cgactccagg agtgctcttc gatctcgcac agaaggaagg gatcactgtg ttcggaacga
      121 gtgcgaaata tctctctgct ctggaagccg caggtgtggt tcctcaaaag gagttcgatc
      181 tatctcgtct tcgcacgatt ttgtctacgg gtagtccgct ttcgcgcgaa agctatcggt
      241 atgtgtacga tcagatcaaa gcggatgttc aactctcttc catttccgga ggcacggatc
      301 tcaacggttg ctttgctctg ggcaatcctg ttcttcctgt gtatgaaggg gagctgcagt
      361 cgaggggtct tgggatggct gtggaagtgt gggatgattc gggaaaaagc gtgaaggaac
      421 aaaaaggcga acttgtatgc attcgagcat tcccatccat gcctcttttt ttctggaacg
      481 atccagacgg ttcacgatat cacaaagcat actttgatca tttcccgggt atctggaggc
      541 atggcgattt tgcgatgatc acagaacaca acggaatgat catttacgga agatcggacg
      601 ccactctcaa tcccggtgga gtgcgcattg ggactgcaga cctatatcga attgtagaat
      661 cgtttggaga gatcgaggat tctgtggtgg tgggacagga atggcagaat gaccagagag
      721 tcattctgtt tgtgaagatg aagcagggtt ctttaacaga agatcttaag aaacaaattt
      781 cgcaaaagat tcgagattcc gtttctccaa gacacgttcc cgcctatatc tttcaggtgc
      841 ccgatattcc ttatactaga aatctaaaga aggtagagct ggcagtgaaa agagtgattc
      901 aggatgagcc agtcacaaac agagacgcac tcgtgaatcc tgagtgcctt gaattttaca
      961 aaaacattcc agaacttcgg agtcagtcat gaatgtatac gatctgccca ttcgtccgcg
     1021 aagaaatcgc aggacagaat ccattcgcag cattgtgcga gaacattccg tttcgagtac
     1081 ggacttgatt cagccaatgt tcgtctgtga agggaatcaa aagagcgagg caatcgtttc
     1141 gatgccgggg atttttagac tcagcacgga tctcattgtc aaagagtgcg tcgcgctttt
     1201 cgaactgggc gttcccgggg ttgcgctctt tcccgcaatt tcggattcca aaaaagataa
     1261 gaaggcaacc gaatctctaa acgatgatgg cctccttcaa acaaccgtgc gcgccatcaa
     1321 ggccgcggtt cccggaatca ctgtgatcac agatgtggcc atggatcctt acagttcaga
     1381 tggtcatgac ggatttgtaa acgaacaggg cgaaattgta aatgacgtca ctcttgacat
     1441 tctggcgaac atggccgttt ctcaggcccg ggccggtgcg gatatggtgg ccccgtctga
     1501 tatgatggac ggaagagtgg gagtcattcg cgaagcgctt gatgatcacg gttttacaaa
     1561 tgttgggatt ctttcctatt ccgcgaaata tgcgtcttcg tattacggcc cgtttcgcga
     1621 tgccctttcg agtgcgccaa agtcggggga taagaaaacc tatcagatgg atcctgccaa
     1681 tgttcgcgaa gcgatttatg aagtcgaact cgacatagcg gaaggcgcgg atatggtcat
     1741 ggtgaaaccg ggattgccgt acctggacgt tcttcgcgct gtttctgaga tttcaaatgt
     1801 gccggtcgcc gtctacaacg tgagcggcga atacgcaatg ttgcgcgcgg ccgcagagaa
     1861 aggatggctg gattaccaga aagcagttct tgaggcactt ctttctttta aacgcgccgg
     1921 tgctgatatg attttgacct atcatgcgaa ggaagcagcg gagtggctca aatcaaaatg
     1981 aacgcggacg taattgtaat tggtggcggg ctttctggcc tcaatgcagc tcgattgatt
     2041 catcgcgcgg gaaaaaaggt gctcgttctg gaagcgcgtg atcgcgtggg cggacgcacc
     2101 ctttccaaga aatttggaaa tagcgtcatt gatcttggcg cacagtgggt ggggccaact
     2161 caggatagaa ttttgcgact ggcagaggag cagggtcttc gaacattccc gcagcatacg
     2221 ggtggtcgtc aaattctagc gctcggtgac aaagtctcca catacacagg cacgattcct
     2281 tctttgcccg ttcattctct cattgaactg catctgcgaa ttacaaagat agaaaaggaa
     2341 tgcagacgcg tacctctcga agcgccgtgg actttccctg atgcggaaaa gtgggatgcg
     2401 atgacggttg aatcctggaa acagaatcat gtgtatactc gcggagcgcg cgcgagtctc
     2461 gatgcggcgg tgcatgctat tttcgcatgc gaaccatcag agatatcatt tctatacttt
     2521 atgcactact tacattccgg tggcggcctg atgcgcctgg cagatgtgaa aaacggagcg
     2581 caacaggacc ggatcttcgg cggaacacag cagatctctt ctcgtatggc cgcggatctg
     2641 ggtcggcgcg tgattctctc ttctccggtc cttagagtag aacaaacatc cagaggggtg
     2701 actgtttaca ctcgcaaaac taaatacagt gccagaagaa tcatcatcgc tctttctcct
     2761 actctttgtt cgaaaattgc attttcgccg gcgctgccag tggcgcgcga ccagttgcat
     2821 catcgcatgc ccatgggatc ggtcatcaag accttcgcat tgtatgatcg tccattctgg
     2881 agagagcgcg gctattcggg cgaagtggtg agcgacggaa catgcgttcg ccttgcattt
     2941 gatgcgagca ttccgggatc tatgcatgga cttctggttg gattcatttc cggggaaaac
     3001 gcgagagagt ggtcggaccg gaccccgaga gagagagaag ctgccgtcgt gtctgatttt
     3061 gttcgcttct ttggagaaga ggcgagcgat cacagaggat acctggaaca aaactggatt
     3121 gcagaagaat tcagtcgggg ctgctacgcc ggccttatgc ctccttctgt gatgacgtca
     3181 ttcggcaatg ctctgcgaga tccgtgcgga ctcatccact gggcaggcac agaaacagcg
     3241 cggcactgga atggatatat ggaaggagca ctcgaggcag gagagcgcgc cgccgcagaa
     3301 gtactcgagg cgcgctgatt gcagactaag ccgcggcttt ttgctgactt tgatcgatga
     3361 cgatcgttcc cgaccagcga tcatgcagag tgcgatgttg cggggaaaat agaaagaata
     3421 cgtctagaaa gtatgtaaag ccaaacctat tttgtaacca acgacacaat cgccttacat
     3481 gtgtttgtag ctggcgcctt tttcgagtag atccagataa ccgctgagct tgtgcctgag
     3541 cgcttctcct atctccacaa ccttttcttt gaatcgattg ttgtaagctg cccgttcctc
     3601 ctcattcaga ttggcaacaa tcccatccac aatcgcggcg cgctgtgatt tatccggagg
     3661 tgtttccata ttcagaattt gttctgcata cagatccgag attttttttc gcacacttgc
     3721 gcgtttgagg aaatcttcct cggagaatcc aagattctcg agtaccacga ttctaccggc
     3781 atgagatgta ttgtccagca ctgcgtcttc cagctctaga tccgctgaat tcatcagtgc
     3841 gaccagttcc ttttcggaaa gccagctgat gagacgttgc agtttgactt tgttcaacgc
     3901 ggatattctt tcatagaaat caggttcgcg atacatcatc cggcggcctc cggaaggatt
     3961 cgtccctggc gtactgtgac cggggaacct tctgctacga aaacagaggc gggcgttttt
     4021 gacagagggt ctacaaattc cggtccgtac gggcgtgcaa catctacgtc tactctgggg
     4081 tctttgcagg aaagaacgcg ccattctgga tgttcaactc tatactcaac cgtttgaccg
     4141 gccgggcttt tcacatatcc aaagtaatgt tcaatgataa aatgttcctg cgatcccgca
     4201 gccgggtagg aagggttccc tgtgcatttc gcaccaatcg aatgcctctt ttcgtctaac
     4261 catgagtaga cgactctgcc gttcactgca agcgcgctat ttttaatatc cagaatgtga
     4321 ctcatgggat gtgacacgta tcgctctcca tatgcttttc gagcaaccag cgcaattgcg
     4381 tatctgggga caatttcttt gaggaagcag actcctctcc tggtttcctc tccttctttt
     4441 cttttcacgt agaatctaag attgatttct tcaaagttgg tgtgaaatgg aatgggcagt
     4501 tttcccagaa ggcgagtgcg aagaaacata aacccgacca gactcacgaa gcattttccg
     4561 ccgaatagat ccagctctac tccgcgtgga acgtggggtg ccagtatgtc cggattcact
     4621 tcatagttca caagcaataa atgacgccag tctgccgtca ggaaaatttt gccatccgtg
     4681 tttgcttgct tttgcgatga tgcttccata gattccgtta gacgaatttc aagctacctt
     4741 gttcgaggcg acgataatag caggagtatc tatatttatc cttttctttt gtgtggcagg
     4801 caccctggcc ctcgagttct gcgagaaata ggagagagtt ttgttcgcag ttgacccgga
     4861 tctccttcaa tcgcaaacga ttgcctgaag tgtcaccctt tacccagatt tcttttcgcg
     4921 acgtgctgaa gaaagtgacg aatccagttt caatgctttg cttcagtgcg gcatcgttta
     4981 cgtatccaag gattaagacg tctttggtct tcgcgtcctg aacaatgact ggtaagactg
     5041 cacctgcatc ggctgccttc ctggtttttt caaaatccag cgagagggtt ttgccttctt
     5101 ctacttcgat catggagcaa cactgtttgt gtgacgcccg gctgcatctc gaaattctgg
     5161 tcgtaatcct gccgggcaga caaaaaataa tcccgtgcag gaacttcttc tcaagcgcca
     5221 tagtgtcttt cgcggcgatg tgacgctccc cggatcaaaa tcgatcgcca atcgcgctct
     5281 tttgctctca agcctggcac agggaaagac gatcctgcga ggtctgccag atgcagatga
     5341 cgtgactgtg tttcgccgca accttccgaa cctcggaatt caggcgaccg agatgggaga
     5401 ctctctggaa attgtgggtg cgggaggttc ttttcccgtg caaaaagccg acatctttgt
     5461 tgagaacgca ggtacggcca tgcgacctct tgcggccgct ctctgtgccg ggaaaggcga
     5521 ttttgtaatt gatggcaatg aacagatgcg acgtcgaccg attggagatc ttgtgcgcgg
     5581 tctggcagaa ctgggagtag atatcacagc tgcgcaggca accgtgcagg ggaagctgga
     5641 aatggttcct cctgtaacca tacacgcgaa aggaattgcg ggggggcgaa cgcgtctttc
     5701 tggaaacgta tcgagtcagt tcatttctgc agtgctactg gcagcgcccc tggcaagcaa
     5761 gcctctgaca ctggaagtgc caggagatcc tgtaagcaaa ccctacatag atatgaccat
     5821 ttctatgatg aagcaatttg gcgctggtgt tcgaagggaa cgttattcgc tttttgaaat
     5881 tgcgccgcaa aggtatattt ctcctggaga gtttactata gaaggagatg caagcgctgc
     5941 cacttacttt ctatctctgg gcgctctcag cgggcctgtg actgttcgca cgatctactc
     6001 tgattcagtg cagggagatg ttcgatttgc agatgtgctc tcgcggatgg gcgcggtggt
     6061 aactcgttta gatgattcca ttacagt
//
LOCUS       DAORFF010000184         6082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6883_length_6082_cov_4.306620, whole genome shotgun sequence.
ACCESSION   DAORFF010000184 DAORFF010000000
VERSION     DAORFF010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6883_length_6082_cov_4.306620"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PKK76_08825"
     CDS             <1..890
                     /locus_tag="PKK76_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNL68618.1"
                     /translation="TVFMVKVSFEQKPYRKNLMEIFGAKVIASPSTQTDAGKKILAND
                     PSCTGSLGIAISEAVEVAAKDPGTRYSLGSVLNHVLLHQTIIGLEAEQQFLLAEDEPD
                     ILIGAFGGGSNFAGFTFPFLRHKIAGKKMRVIASEPAACPTLTRGKFAYDFGDTVGLT
                     PLVAMHTLGHNFVPSAIHAGGLRYHGAAPLVSQLLVDNLIEAVAYKQNECFASAMLFA
                     KNEGIVPAPEAGHVIHAAVVEALKCKESGESKVIAFNLCGHGHFDMSAYQAYMAGGLK
                     DFELSDKELQENLAALPGV"
     gene            complement(954..1334)
                     /locus_tag="PKK76_08830"
     CDS             complement(954..1334)
                     /locus_tag="PKK76_08830"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNL68619.1"
                     /translation="MCLLYPVTIAWVVLLHFLEVTLFLNLMSAATGLLWGYLMFSLVH
                     RNFHIEGTWFLKTAFTRKWFRHVRRLHDIHHHKLTNEGRMPYNMGICFYWFDRLFGTY
                     RPALGRFNSRGLEKSRELYAHLYK"
     gene            1408..1566
                     /locus_tag="PKK76_08835"
     CDS             1408..1566
                     /locus_tag="PKK76_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68620.1"
                     /translation="MTRAIIKDVVMHMSFAQELHLFAVEESVDPVSEKFGGYNRRQKC
                     ECYLHAVQ"
     gene            complement(1643..1858)
                     /locus_tag="PKK76_08840"
     CDS             complement(1643..1858)
                     /locus_tag="PKK76_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68621.1"
                     /translation="MKRIMALTLALWSGGCGINHWYNHRVKHCQLEAAVLSVAACSGI
                     TDANQRSACSAIAGAILYTECFEHDND"
     gene            complement(1855..2601)
                     /locus_tag="PKK76_08845"
     CDS             complement(1855..2601)
                     /locus_tag="PKK76_08845"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNL68622.1"
                     /translation="MRILAALAIAFTLTSKIAAAEKPVAVVISVQGDRALIQNSQSFD
                     LEPSDLIRAGDEIQTGADGKVSVQSSTGVIFQIGSLSHVKITDMNQTSDSSVLIVDLK
                     TGNIATIVSKTSGSNSVRIQAPTAAASVRGTEFLVEADEEETSVSVGDGEVEVEDAGG
                     TKARVRPGGVIRARLREKLREERIDARRAERLKVFGEFRTKRDEKFRERVEKLRNTKE
                     RIRKRREFIRNLREARRDSLKEAREERKKR"
     gene            2741..4813
                     /locus_tag="PKK76_08850"
     CDS             2741..4813
                     /locus_tag="PKK76_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNL68623.1"
                     /translation="MGIQSDVYRSFFDRSIEGMFLTTPAGRYILVNSRLAEIYGFSSS
                     EEMMDHFQNIKTQLYVDPSRRDDFVKAMNEAGTVLEFESQVRRKDGTIIWISENARQV
                     RDEHGQIVHYEGTVVDITDRKLLAGTMARQQAFYHQLFHNSPLAMILLDAERSVISCN
                     SAFERLFGYNASEIRSSSIRDKIIPDELILEAENYRAAILTGSSHTKETIRRHKDGRD
                     IPVNMQGFPVMLEGSVAGIYYLYEDITERKAYEETIMRQAFHDSLTGLPNRHLFNDRL
                     NRAVERSKRRESYHFAVVLMDLDRFKKVNDTLGHLVGDRLLVHVGKILHSCLRTVDTA
                     ARLGGDEFALIEEFRSRQDVLLVLDRIHALLHEPFDVGERTLQTSGSMGIVINTSEYS
                     SAEELMRDADIAMYRAKEHRKPYQFFSREMQRELMEIMEIETDLKNAIAGQQLFLYYQ
                     PIVSLARKRLEGFEALLRWMHPSRGMMQPDHFIPIAEDTGMILPIGSWVIQEACRQLR
                     VWTDANRLWDDLTISINVSIRQFTHIDLPGEIERSLSLYEIDPGRLRLEITESTLIQD
                     MDEILAVLRELKRLGVKLAIDDFGTGYSSLSYLRELPVDTLKIDRSFISGENRSPDSF
                     QIVKLVTSMARSLGMTVVAEGVEEVYQQDILRDLQCDNAQGFLYSRPLKGDGVAEWIL
                     ENGFLEQR"
     gene            complement(4820..5287)
                     /locus_tag="PKK76_08855"
     CDS             complement(4820..5287)
                     /locus_tag="PKK76_08855"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNL68624.1"
                     /translation="MQRLHPRGIKNGTITPFIWFDTDVQEVAKFYNGIFKNSKVLSTN
                     PMSATIVINDQKLHLFNGGPHYKLNEAFSLMVSCETQKEIDYYWDKLLAGGGQESRCG
                     WLKDKYGLSWQIIPDVLGQLIGDKDRQKADRAMQAMLKMAKLDLAALKAAHQG"
     gene            complement(5268..5765)
                     /locus_tag="PKK76_08860"
     CDS             complement(5268..5765)
                     /locus_tag="PKK76_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNL68625.1"
                     /translation="MLGKRNLTITAKSDLEIVWTRAFNAPPEFVFEAFTTPALVQRWL
                     LGPDGWTMPVCEIDLRVGGSYRYVWSHAQQGTMGVGGVYREIKKPSRIVQTEKFDQAW
                     YPGEALQTLELKKEGSMTWMTATALFETKEARDAALNSNMEKGMVPSYDRLDEIAEQN
                     AKAAS"
     gene            complement(5719..>6082)
                     /locus_tag="PKK76_08865"
     CDS             complement(5719..>6082)
                     /locus_tag="PKK76_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNL68626.1"
                     /translation="ALADPTRRAILMRLAKGDVSVMALAKPFAMSQPAISKHLKVLEQ
                     AGLISRSQDGQKRPRKIESSAFALADEWLDKFRKLWEYRYEQLDFLLEEMKKTRPTKR
                     REKYAGKKKSDNNRQKRS"
BASE COUNT         1444 a         1501 c         1522 g         1615 t
ORIGIN      
        1 gcaccgtgtt catggtaaaa gtttcttttg aacaaaagcc atatcgcaaa aatctcatgg
       61 aaatctttgg cgcaaaagta atcgcgtctc cgtctacaca aacggatgca ggaaaaaaga
      121 ttcttgcgaa cgatccttcc tgcactggct ctcttggaat tgcaatttca gaggccgtag
      181 aagtggcagc aaaagatcca ggcacccgct actctcttgg aagcgtgctc aatcacgttc
      241 tgctgcacca gaccatcatt ggtctggaag cagagcagca gttcttgctg gcagaagatg
      301 agccggacat tctcattgga gcgttcggtg gaggatctaa cttcgctgga tttacatttc
      361 cattcttgcg ccacaagatt gctggaaaga aaatgcgcgt catcgcatct gagccggctg
      421 cctgtccaac gctcactcgc ggaaaattcg cgtacgattt tggagacact gtcggcctta
      481 ctccgctcgt tgccatgcac actctgggtc ataattttgt tccaagtgcg attcatgcgg
      541 gcggtcttcg ctatcacggc gcggctcctc ttgtaagcca gctccttgtg gacaatttga
      601 ttgaagcagt tgcttacaaa caaaacgaat gctttgcctc tgcgatgctt tttgcaaaga
      661 acgagggaat tgttccggca ccagaagcag gtcatgtgat tcatgccgct gttgtggaag
      721 cgttgaagtg caaggagtcc ggagaatcaa aagtgattgc ctttaacctc tgcggtcatg
      781 ggcatttcga tatgtccgcc taccaggcat acatggcagg cggactcaaa gactttgaac
      841 tttcagacaa ggagctgcaa gaaaacctgg cagcattgcc aggcgtttaa tcgctcctgg
      901 cgcttcgttg ggcgcctgac tgttgttagg tgaggcgagc catccggctc gtctcactta
      961 tatagatggg cgtagagttc gcgtgatttt tccaggccgc gcgaattgaa tcgtcccaga
     1021 gcaggcctgt aggttccaaa gagcctgtca aaccagtaaa aacaaattcc catgttgtag
     1081 ggcatacgac cttcgtttgt caacttgtga tgatggatgt cgtgaagtct gcgaacatgg
     1141 cgaaaccact ttcgcgtgaa tgcggttttt aggaaccacg tgccttctat gtgaaaattt
     1201 ctgtgcacca gactgaacat gagataaccc cagagtagac cggtggcggc ggacatcaga
     1261 ttgagaaaca gtgtgacttc cagaaaatga agaagtacca cccaggcgat cgtgaccggg
     1321 tacaagaggc acaagccatt ctgcgcccag accaaagaaa gtgaaacgcc cgatgcctga
     1381 atccttgtaa tcctgcgatc tctgcgaatg acgagggcca taatcaaaga tgtggtgatg
     1441 catatgagct ttgctcagga acttcacctt ttcgctgtgg aggagagtgt ggatccagta
     1501 tccgagaaat tcggcggcta caaccgacgc cagaagtgtg aatgctatct tcatgctgtg
     1561 caatgagtca gaaggtgacg gattgtcaag ccaaaacatt aacatcgtta accagccaga
     1621 ggccaggtaa ccttccgcaa tttcagtcgt tatcgtgttc gaagcattca gtgtagagaa
     1681 tcgctcctgc aatggctgag caggcgctgc gctggttggc gtccgtgatg ccactgcaag
     1741 ccgccacaga cagaacggct gcttcgagct ggcagtgttt cactcgatgg ttgtaccagt
     1801 gattgatccc acagcctccg ctccataagg cgagggtaag tgccatgatt cgtttcatct
     1861 ttttttcctt tcttcgcgcg cttccttgag agagtctcta cgcgcttcgc gcagattgcg
     1921 aatgaactcg cggcgtttcc taattctttc tttagtatta cgaagtttct ccactcgctc
     1981 gcggaatttc tcatcgcgct tcgtgcggaa ttctccaaaa accttgagtc tctctgcgcg
     2041 cctcgcgtcg attctctctt cgcgcagctt ttctctcaat cgagcgcgga tgactccgcc
     2101 tggcctgaca cgagcttttg ttccgcccgc atcttctact tctacttccc cgtcacccac
     2161 agatacactg gtttcttctt catctgcttc caccaggaat tctgttccgc gcacgctggc
     2221 cgcggcagtg ggtgcctgga tgcgcaccga gttggatcct gaagttttac ttacgatcgt
     2281 tgctatgttg ccggtcttaa gatccacaat caatacgctg gaatcagaag tttgattcat
     2341 gtctgtgatc ttgacgtggg aaagagaacc gatttgaaat atgacgccgg tggaagattg
     2401 tacagatact ttcccatccg cgcccgtttg aatctcatct cctgcacgaa tgagatcaga
     2461 gggttccaga tcaaaagact gtgagttctg gatcagtgcg cgatctccct ggacgctgat
     2521 gaccacagcg accggctttt ctgcagcggc gattttggag gtcagtgtga aagcaattgc
     2581 gagtgctgcc aggattctca tgcaacgatt ttgtccaggt gcgagcacag gagtcaatcc
     2641 agacttggaa ggtcggcgcg cgttttttct gaatcgcacg gattttttcc aaaaaatcat
     2701 ctccattcga gaatggcacg ctatatgcat tgaatgagtc atgggcattc aatcagacgt
     2761 atatcgaagt tttttcgatc gctctattga aggaatgttt ctgaccactc ctgcgggcag
     2821 atacatactc gtgaattcca gactcgccga aatatacggc tttagctcta gcgaagagat
     2881 gatggatcac ttccagaaca tcaaaacgca gctgtatgtc gatccttcca ggcgtgatga
     2941 cttcgtaaaa gcgatgaatg aggcaggaac cgttctagaa tttgagtctc aagtgcggcg
     3001 aaaagacgga acgatcatct ggatttcaga aaatgcccgg caggtgcgcg acgagcatgg
     3061 acagattgtt cattatgaag gcacagtggt ggatatcacg gatcgtaaac tgctggcagg
     3121 aaccatggcg cgccagcagg ctttctatca tcaattgttt cacaattctc ctctggcgat
     3181 gatccttctg gatgcagaga gaagcgtgat ctcatgcaac tccgcctttg agcggctatt
     3241 cggatacaac gcatctgaaa ttcgttcttc ctcgattcgc gataagatca tcccggatga
     3301 attgattctc gaagctgaga actatagagc ggctatctta accggaagca gtcacacgaa
     3361 agaaacgatt cgtcgccaca aagacggccg tgatattcca gtgaacatgc aaggatttcc
     3421 agtgatgctg gaaggctccg tggctggaat ttattatctc tacgaagaca taacggaaag
     3481 aaaagcatac gaagagacaa tcatgcgtca ggcgtttcat gattcactca cagggcttcc
     3541 caatcgccat ctattcaatg atcgcttgaa cagagctgtg gagcgaagca agaggagaga
     3601 gtcgtatcac ttcgcggtcg ttctcatgga cctggatcgt ttcaaaaaag taaacgatac
     3661 tctgggtcat ctagtgggtg accggcttct ggttcacgtt ggaaagattc tacattcctg
     3721 tcttcgcaca gtggacaccg ccgcgcgcct gggtggagac gaatttgctt tgattgagga
     3781 attcagatcg aggcaggatg tacttcttgt gctcgatcgg atccacgcat tgctgcacga
     3841 accgtttgat gtgggagaga gaactcttca gacttcggga agtatgggca ttgtcatcaa
     3901 tacttcggag tattccagtg cggaagagtt gatgcgcgat gcggacattg ccatgtatcg
     3961 cgcgaaggaa catcgaaaac catatcaatt tttcagccgc gaaatgcaac gtgagttgat
     4021 ggagatcatg gaaatcgaaa ccgatctcaa gaacgcaatc gcagggcagc aactttttct
     4081 gtactaccag cccatcgttt ctctcgcgcg aaagagactc gaaggatttg aagcactcct
     4141 tcgatggatg cacccttcgc gcggtatgat gcagccggat catttcattc cgatcgcaga
     4201 agacaccgga atgattctgc caatcggtag ctgggtgatc caggaagctt gcaggcaact
     4261 gcgtgtgtgg acggatgcaa atcgtctctg ggatgatctg accattagca tcaatgtgtc
     4321 cattcgtcag ttcacacaca ttgatctgcc tggagaaatt gagcgaagtc tctctctgta
     4381 cgaaattgac ccgggccggt tgagacttga gattacggag agcaccctca ttcaggacat
     4441 ggatgaaatt cttgctgttc tcagagagct caaacgtctg ggagtcaagt tagcgataga
     4501 cgactttggg accggttact cttcgctcag ttatcttcgc gaacttcccg tggacactct
     4561 gaagattgat cgctctttca tcagtggaga aaacagaagc cctgattcat ttcaaatcgt
     4621 gaagttagtc acttctatgg ccagaagtct tgggatgacg gttgtcgcag aaggcgtgga
     4681 agaagtctac cagcaagaca tcttgcgcga ccttcaatgc gacaatgctc agggctttct
     4741 ctattccaga ccgctcaagg gagacggggt tgcagaatgg attctggaga atggcttcct
     4801 ggaacaacgt taagcggcat taaccctgat gagctgcctt cagagccgcc agatcgagct
     4861 ttgccatctt gagcatggcc tgcatggctc tgtctgcttt ttgtctatct ttgtctccga
     4921 tcagctgtcc tagaacatct ggaatgatct gccatgatag tccgtatttg tctttgagcc
     4981 agccgcagcg actttcctgt ccgccacctg ccaggagctt gtcccagtag taatcgattt
     5041 ccttttgagt ctcgcagctt accatcaaag aaaatgcctc gttgagtttg tagtgcgggc
     5101 ccccattgaa cagatgcaac ttctgatcgt tgatcacaat cgttgcggac attggatttg
     5161 tgctcaatac tttagaattc ttaaatatcc cattgtaaaa ttttgcaact tcctgtacgt
     5221 cagtgtcaaa ccagataaac ggtgtaattg ttccattttt tattcctcta ggatgcagcc
     5281 tttgcattct gctctgcgat ttcgtccagt ctatcgtaac tcggtaccat tcctttttcc
     5341 atgtttgaat tcagagccgc atcccgggct tcttttgttt caaagagtgc agtggcagtc
     5401 atccatgtca tgcttccttc tttcttgagc tctagagtct ggagggcttc tccaggatac
     5461 caggcctgat cgaacttttc agtttggacg attctggaag gcttcttgat ctcgcggtat
     5521 acgcccccga ctcccattgt gccctgttgc gcgtggctcc acacgtatct atagcttcct
     5581 ccgacgcgaa ggtcgatttc gcagacgggc atggtccaac cgtccggacc gagtaaccac
     5641 cgttgtacga gcgccggcgt tgtgaacgct tcaaaaacaa attcaggtgg cgcattgaat
     5701 gcgcgcgtcc aaacgatttc aagatcgctt ttggcggtta ttgtcagatt tctttttccc
     5761 agcatatttt tctctcctct tggttggtct ggttttcttc atttcttcca gcaagaagtc
     5821 cagttgctcg tatctgtatt cccagagttt cctgaactta tctaaccatt cgtctgcgag
     5881 cgcaaaagca ctgctttcaa tcttgcgtgg acgcttttgt ccatcctggc tgcgagaaat
     5941 gaggcccgcc tgctcgagca ctttgagatg cttcgatatg gccggctggc tcatcgcgaa
     6001 gggtttggca agtgccatca cagacacgtc cccttttgcg agtctcatta agatcgcgcg
     6061 ccgggtagga tccgcaaggg ct
//
LOCUS       DAORFF010000185         6061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6929_length_6061_cov_3.820013, whole genome shotgun sequence.
ACCESSION   DAORFF010000185 DAORFF010000000
VERSION     DAORFF010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6929_length_6061_cov_3.820013"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PKK76_08870"
     CDS             complement(<1..565)
                     /locus_tag="PKK76_08870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNL68627.1"
                     /translation="MLASDSMTALSDPNDWVERHGSLLFSFALTRVRDRATAEDLVQE
                     TLLAALRSRGNFQGRSSESTWMVGILKNKIMDHFRKTSREVDHPLPFENEQLYETTGD
                     WAGHWKEGLTPMHVSPETETEMQELRAKLDSCIQELPGRIASIFTLRELDHAKTEDLC
                     KEFSISPTNLGVILYRARAQLRLYRGSH"
     gene            640..1245
                     /locus_tag="PKK76_08875"
     CDS             640..1245
                     /locus_tag="PKK76_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68628.1"
                     /translation="MRYAAVILCLAMVGCPRNEDDAIMLHLYHKLHVEIERATMGTDI
                     APAYLAAIVSLESHPPGNRSSERFEPYVYQRLVDLKNDNRAFGKIKRESLVFMSDAEI
                     RQLATSYGLTQVMGFHCLELGCSIQDLRGPYHLQWAVGYMMRHYGPHARKRDWNACFR
                     IHNTGLPDGTPARKDYVDQGLMRMQYYQRWMDTRGDVLTFN"
     gene            1249..1953
                     /locus_tag="PKK76_08880"
     CDS             1249..1953
                     /locus_tag="PKK76_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNL68629.1"
                     /translation="MKAYAGTSGFSYKEWKGPFYPEKIKNDAMLAFYSQQFSTVEINN
                     TFYKMPASKTLETWVEQTPDTFRFSIKASQKITHHQRLKQSDDSVAYLFKQLVHLKDR
                     LGPVLFQLPPYLKKDMERLNSFLEVLPQDVFCAMEFRNAEWFCDEVYDSLRSHNVALA
                     FADGEVEGEQFVATSDRGYVRLRHEQYDEKSIAEWVRRIKSQNWKEAFVYFKHEDAGT
                     GPRLAAEFQRQFQTIG"
     gene            complement(1962..5639)
                     /locus_tag="PKK76_08885"
     CDS             complement(1962..5639)
                     /locus_tag="PKK76_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL68630.1"
                     /translation="MIFLTAHTTANIILVIFNLLVGGFLILRPGRTTASFHLGLGFIF
                     MAVFPSGYVFGTSLYHPIGAFHRYMTVSFVVPLCMQFGQFFLRYPANEHRRGANALLA
                     VGWIVSASLSIYFILSTFYGPVEFEFSSGVFNFVADRPTRIVGLFILAAVVHIIFVLG
                     YRILRKDPESKFLTALAALLIGSSFPTAIVNYLVTLGYFDRGFFFNMFIFNILTFFFG
                     FVILYINHTHERSTFMAKLVGITVVTVLLLVQSFSYYTFGKAERSFDETMLEQSIRLA
                     SDPRQTGAASYVATMEPLGNLRWSSLAASPDIEMTKNAIANARWIETLRQKTAAPAER
                     PDSFLLYDAILAERRKANPSISTEDLIGELVTLDAPLSGLRRGLEVIPVSEFDRRAPE
                     LIRSFRDKTVATRLMQAMEDGPASEHKNAVTRLLSPALPILSRRYAEARTADVASRER
                     FVTYVVRNPNGEFVEVAFPYLLYRSQIHSTAKFLLLELGAAMLVILFLYPLFFRGSLI
                     NPLRTLISGVQRVNSGDLSVSVPVRIEDEIGFLSRSFNNMVTSIRDARDNLEQKIRDR
                     TEDLQKALDKLREADQIKTNFFANVSHELRTPLTLMLAPLESMRAGEVGPLTPTGASF
                     VDSMLKNGEKLLKLITNLLDYSKLEAGRMQLNYGDAEMVAYVRELVSSFDSAARTRGL
                     FLEVHTAAPQIRAQIDPDKMEKIILNLLSNAFKFTQEGGISLYLSEENGIVQIVVKDT
                     GIGIPEEKLQTIFERFSQVDASASRKYEGTGIGLALARELAILHGGDLSVTSRVGAGS
                     EFVIRFPASGEGKADLQTVTNSRPQTARAGVLAEVELENESSNHDPDTMVELTTQEVQ
                     DTRLVLIVEDTHDMRKLLYFFLKPHFRVVTARNGREGLEKARTHRPALIVSDVMMPEM
                     SGYDLLRAVRSEEELKTTPLILLTARADVNMKVEGLDQGADDYLVKPFNSRELLSRIR
                     AQLRIQAMQDEIRTMRDRLLEVNEQLSGKVTEQASEIFLKEKFRNYLPPQLVDAILSR
                     EGDAQVRSTRKKLSIFFSDIVQFTRITGELEPEDIARLLNHYLSEMTKIARKHNGTVD
                     KFIGDAVLIHFGDHGSRGEKQDAIDCVQMAIDMQLRMRELSAEWIEQGFEEPFQIRCG
                     INTGYAAVGNFGSEDRLDYTIIGSNVNLASRIQSNAEPGHILISHATWALARDHFRLE
                     PRDNIAPKGFNRTIPVYAVKF"
     gene            complement(5714..>6061)
                     /locus_tag="PKK76_08890"
     CDS             complement(5714..>6061)
                     /locus_tag="PKK76_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68631.1"
                     /translation="QAFWASVLLCFASLAKNAYETVIDFFAFTGAFFNVLTFLALFVL
                     RKKFPDAPRPFKMPLYPLPALIAIAVYVMLMISALFTAPLESLSGVALTASGLLYYRY
                     LRKGARSDLLQNH"
BASE COUNT         1565 a         1425 c         1578 g         1493 t
ORIGIN      
        1 ggtgtgatcc tctatagagc ctgagctgag ccctggctct atagaggatc acacctagat
       61 tggttggact gatcgagaat tccttacaaa gatcttccgt tttagcgtgg tccagctctc
      121 gcaaagtgaa gatagaggca atgcgccctg gcaattcctg aatgcaggag tcaagttttg
      181 cacgcagttc ctgcatttcc gtttcagttt ccggagatac atgcatgggt gtcaggcctt
      241 ccttccagtg accggcccaa tcgcccgtgg tttcgtagag ttgttcattt tcgaatggga
      301 ggggatggtc tacctctcta gaagtctttc gaaaatgatc catgattttg tttttaagaa
      361 tgccaaccat ccaggtgctt tcggaacttc taccctggaa attgccgcgg cttcgcaatg
      421 cggcgagaag tgtttcctgt accagatctt ccgccgtggc tctatcacga acgcgtgtca
      481 aagcgaagct gaagagcaga ctgccatgtc tttcgaccca gtcattcgga tctgagagtg
      541 cagtcatgct gtctgatgcg agcatcaggc agaaaacttc aagagaaaaa attgttcaaa
      601 gtcatttgac atcgctttag cgcgtgcaac caataccctg tgcgatacgc ggccgttata
      661 ctgtgcctgg ccatggtagg atgtcccagg aatgaagacg atgcgatcat gttgcatctc
      721 tatcataagt tgcatgtgga aattgaaagg gccactatgg gcacggacat tgccccggca
      781 tacctcgctg ccattgtttc cctggaatcg catccgcccg gaaatagaag cagcgaaaga
      841 tttgaaccct acgtgtatca aagactcgtt gatttgaaaa acgacaatcg cgcgttcggg
      901 aagatcaaga gagagtctct ggtctttatg agtgacgcgg agattcggca gctggccaca
      961 agctacggtt tgactcaggt gatgggattt cactgcctgg aactgggttg ttcgattcaa
     1021 gatttgagag ggccgtatca tttgcagtgg gccgttggct atatgatgcg tcactatgga
     1081 ccacacgctc gaaagagaga ttggaatgca tgctttcgca ttcacaacac tggactgccg
     1141 gacgggacgc cagcgcgaaa agattacgtg gatcagggct tgatgcgaat gcaatattac
     1201 cagaggtgga tggacactcg tggtgatgtg cttacattca attgaactat gaaagcatac
     1261 gcaggaacaa gcggtttctc ttacaaagaa tggaagggcc ctttttatcc ggagaaaatc
     1321 aagaacgatg cgatgctcgc gttttactcg cagcagttta gcacggtaga gatcaacaat
     1381 acgttttata agatgccagc atcgaaaacc ctggagacct gggtggagca gactcccgat
     1441 acgttccgat tttctatcaa agcttcgcag aaaatcacgc atcatcagcg cttgaagcaa
     1501 tcagatgatt ccgttgcgta cctatttaag cagcttgtgc atctgaaaga tcgtctgggt
     1561 cctgtgctct tccagcttcc tccctatttg aaaaaggaca tggaacgatt gaattcattt
     1621 ttagaagtac tgcctcaaga tgttttttgt gcgatggagt tcagaaatgc cgagtggttc
     1681 tgcgatgaag tctacgattc tctgcgttcg cacaatgtgg cgcttgcatt cgcagatgga
     1741 gaagtagaag gagagcagtt cgtggccacg tcagaccggg gctacgtccg gcttcgtcat
     1801 gaacagtacg acgaaaagag cattgcagag tgggtgcgga gaatcaagag ccagaattgg
     1861 aaagaggcat ttgtctattt caagcacgag gacgcaggta ccgggcccag actggccgcg
     1921 gagttccaga ggcagtttca aacgattggc tgagaacgcg cctagaattt gactgcatag
     1981 actggaatgg ttcggttgaa gcccttgggc gcaatattgt cgcgaggttc aaggcgaaaa
     2041 tgatcccgag ccaatgccca tgtggcgtgt gatatgagta tgtgtcccgg ttctgcgttg
     2101 ctttgaatgc ggctggcgag attcacattt gaaccaatga ttgtataatc caggcggtct
     2161 tcggatccaa aatttccgac tgccgcataa ccagtgttaa tgccgcaacg aatctggaac
     2221 ggttcttcaa aaccctgttc aatccattct gcggatagtt cacgcatacg cagttgcatg
     2281 tcgatagcca tctgcacaca atctatggcg tcttgcttct ccccgcgcga accgtgatct
     2341 ccaaaatgga ttagtacagc gtcccctatg aacttgtcca cggttccgtt gtgttttcgc
     2401 gcgatcttcg tcatttcgga tagatagtga ttgagaaggc gcgcgatgtc ttctggctcg
     2461 agttcacctg tgatacgcgt aaactgcaca atgtccgaga agaagatgct cagtttcttg
     2521 cgtgtagaac gaacctgcgc atccccttca cgggatagga ttgcgtcgac aagctgagga
     2581 ggcagatagt tcctgaattt ttctttcagg aaaatctctg acgcttgctc cgtaaccttg
     2641 ccggaaagtt gttcattgac ttcgagaagg cgatcacgca tagttcgtat ttcatcttgc
     2701 atagcttgaa tccggagttg ggcgcgaatg cgcgaaagca attcacgcga gttgaatggt
     2761 ttgaccaggt aatcgtccgc tccctgatcg agtccctcca ctttcatatt cacgtccgct
     2821 cgggctgtga gcaggattaa aggggtggtc ttcagttctt cttctgatct gactgctcga
     2881 agtaagtcgt agccgctcat ttctggcatc ataacatcag agacgatgag cgccggccga
     2941 tgtgttcgcg ccttttccag accttcgcga ccgttgcgcg ctgtgactac tctgaagtgc
     3001 ggcttcaaga agaagtagag taacttccgc atatcgtgcg tgtcctctac gatgagaacc
     3061 aatcttgtat cttgcacttc ctgggttgtg agctccacca tcgtatccgg gtcatggttg
     3121 ctcgattcgt tttcgagttc cacttctgca agaactcccg ctcttgcggt ttgaggtcgg
     3181 gagttcgtaa ctgtttgtaa gtcggctttc ccttcgcccg aagctggaaa tcgaattaca
     3241 aattccgagc ctgcaccaac gcgcgacgta acgctcaaat ctccgccgtg aaggatggca
     3301 agctcgcgag cgagagcaag cccaatgccc gtgccctcat atttacgcga agcagacgca
     3361 tccacctgac tgaatcgctc gaaaatcgtt tgcagcttct cttctggaat gcctattcca
     3421 gtgtccttga ctacgatttg cacaatgcca ttttcctcgg aaagatatag agagattcct
     3481 ccttcttgtg tgaatttgaa tgcattgctc aggagattga ggatgatctt ttccatcttg
     3541 tccgggtcga tctgggcgcg aatttgcggc gccgcggtat gaacctcaag gaagagcccc
     3601 ctcgtccggg ctgcggaatc aaatgaagaa acgagttcgc gtacgtaggc aaccatttct
     3661 gcgtcgccgt agttgagttg cattcgccct gcttcaagct tggaatagtc cagtagattt
     3721 gtaatgagct tgagtagctt ttcgccattc ttgagcatgg aatcaacaaa acttgcacca
     3781 gtgggtgtca gaggaccaac ttcccctgcg cgcatgcttt cgaggggagc tagcatcagg
     3841 gtcagaggag tgcgaagttc gtgcgatacg ttagcaaaga aattggtctt gatctgatcc
     3901 gcctcacgca acttatccag agccttctgt agatcctcag tcctatcacg gattttttgt
     3961 tcgagattgt ctcgggcgtc tctaatggaa gtgaccatgt tattgaagga acgcgaaaga
     4021 aatccgattt catcttctat gcgcacgggc acagatacag agagatcgcc ggaattcacg
     4081 cgctgcactc ccgatatgag ggtcctgaga gggttgatca ggctacctcg aaagaaaaga
     4141 ggatagagaa acaaaattac cagcattgcc gcacccagtt ctaaaagtaa gaactttgcc
     4201 gtgctgtgga tttgcgatcg atacaataga tatggaaatg caacttctac aaattctcca
     4261 tttgggtttc gcactacata cgttacaaat cgctcgcgtg atgcgacatc ggcggttcgc
     4321 gcttccgcat agcgacgcga cagaatcggt aaagccggcg atagcaggcg cgttacagca
     4381 ttcttatgtt ctgaggccgg gccgtcctcc atcgcttgca tcagcctcgt cgcgacggtt
     4441 ttgtcgcgga aggaacggat gagttctggt gctcttcgat caaattcaga aaccggaatg
     4501 acttcgagtc cgcggcgcaa acctgaaagt ggagcatcga gtgtgactaa ctcgccgatc
     4561 agatcttcgg tggaaatgct cggattggcc tttcgccttt cggccagtat ggcatcatac
     4621 aatagaaacg aatcaggcct ttcggctggc gcagcggttt tttgccggag ggtttctatc
     4681 cagcgtgcat tagcaatcgc attcttcgtc atttcaatat cgggagatgc ggccaaactc
     4741 gaccagcgca gatttccgag cggttccatg gttgcaacat agctggccgc acctgtctga
     4801 cgtggatccg aagcaaggcg tatgctttgc tcgagcattg tctcatcaaa ggatctttcc
     4861 gcttttccga atgtgtagta gctgaaactt tgaacaagga gcaacacagt tacgactgtg
     4921 attcccacca gtttggccat gaaagtagaa cgttcgtgcg tgtgattgat gtagaggatc
     4981 acaaatccaa aaaagaaagt taggatattg aagatgaaca tgttgaagaa aaagccgcgg
     5041 tcgaaatatc cgagcgttac cagatagttg acaatggcag tcgggaatga tgatccaatt
     5101 aaaagggccg cgagcgccgt caggaatttt gactcaggat cctttctcag gatgcgatat
     5161 ccgagtacga aaataatatg aactacggct gcgaggatga acagacctac gatccgggtg
     5221 ggtcgatcgg cgacgaagtt gaacacgccg gaagagaatt cgaactcaac gggaccgtaa
     5281 aaagttgaga gaataaaata gatactcagg cttgcggaaa cgatccagcc gactgcaagc
     5341 agtgcattgg cgccgcgcct gtgttcgttt gcggggtatc tcagaaagaa ttgaccgaac
     5401 tgcatgcaca gtggcaccac aaaactaact gtcatatatc gatgaaatgc cccgatcgga
     5461 tgatagagcg aggttccaaa aacatagccg gatgggaaca cggccatgaa aatgaaacca
     5521 aggcccaaat gaaacgacgc agtcgtccga ccgggccgaa ggatgaggaa tcctcccacc
     5581 agtagattaa agatcacaag aatgatattg gcagtcgtat gcgcagtcag gaagatcatg
     5641 ggacgctacg gtaagtccga tggaatctaa ttgtcaagca ttcgaacttt cctgtcagac
     5701 aacaggatga aattcaatga ttttgaagca ggtctgaacg cgcccctttc cgcaaataac
     5761 gatagtacag taaaccactt gcagtcaaag ccactccgct gagtgattcg aggggagcag
     5821 taaacaaagc ggaaatcatg agcatcacat aaacagcaat ggcaatcaaa gccggaagcg
     5881 gatacaaagg catcttaaag gggcgaggag catccggaaa ctttttccgc agtacgaaga
     5941 gagcaagaaa agtcagtaca ttgaagaatg caccggtgaa agcaaagaaa tcaattacag
     6001 tttcgtaagc gttctttgca agagatgcaa aacaaagaag cacgctggcc cagaacgcct
     6061 g
//
LOCUS       DAORFF010000186         6055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6938_length_6055_cov_3.731667, whole genome shotgun sequence.
ACCESSION   DAORFF010000186 DAORFF010000000
VERSION     DAORFF010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6938_length_6055_cov_3.731667"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PKK76_08895"
     CDS             complement(<1..629)
                     /locus_tag="PKK76_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNL68632.1"
                     /translation="MMFDRVFSLFSNDMGIDLGTANTLVYVKGQGIVLSEPSVVAVQS
                     STGKVLAVGNEAKRMLGKTPGDIVAIRPMRDGVIADFETVEKMIRYFIQKVHKRTTFV
                     KPRMVIGVPSGITEVEKRAVRESAEQAGAREIFLIEEALAAAIGADIPIHEPAGSMIV
                     DIGGGTTEIAVISLGGMVISDSIRIGGDEFDEAIVRYLRTQYNLVIGDRT"
     gene            complement(710..1753)
                     /locus_tag="PKK76_08900"
     CDS             complement(710..1753)
                     /locus_tag="PKK76_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68633.1"
                     /translation="MRNYESNTSSRKPLAIASLTLFLIGGAIVAVFYGDQVVAFASGQ
                     ILGSADTRIQKRLALFEQEYKKAGRSPAELLDVVESTRKIVQLMEREKPASAEVYYYY
                     SLLNYYEILVRVPLNGPSLLRMTGRGYLPDPPEGMNLAPVSISALAQETASLARKSLA
                     IDPKPAFASRVRAALILGELMATGRTDPNLLQTTLAIKKEELTPLTQQTTDWMRWALF
                     AMQGKSLELEQEFTERKMLVDPVDGSKPDPDSLDANEARLLLSHACFNAKDYIRALNY
                     ARQVKYNPNTETIWKVDAARLEGEIFLVQSGPIAARPFFEEALKLNGGKDKFLEDRIA
                     SLSPTRPVRTQAP"
     gene            complement(1754..2410)
                     /locus_tag="PKK76_08905"
     CDS             complement(1754..2410)
                     /locus_tag="PKK76_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68634.1"
                     /translation="MYFIGIGLILLGLISLAVLFFSVSTERHQEEPGHIMPVVTRAPD
                     LRDESVRPAPLAPVHPEDAPVSHSQAVAEPSAEPSPSILSHAAVRAVSPVAPAVSDFR
                     PAAKRKDPGQLAVFGTLFLDHARNLSLLSSRGTEDLPPRMFSDFKRVGKGSLVCEGEG
                     FFFKSGNSVHAYSTGDLEQIVFLSSGVALVPGSTQSPIAIFLTDESDRVKAFIRENAA
                     "
     gene            2477..3685
                     /locus_tag="PKK76_08910"
     CDS             2477..3685
                     /locus_tag="PKK76_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68635.1"
                     /translation="MMRVWPMGSVDIIKKFGSVLIIVILGVMLALTFSKTPLSTITDI
                     LSGAGRVGSFDGEEIQPRIYLMAEDNCRQNMSAFGQIPEYILKNCIANQIRQLYVLPL
                     LAHRLGVAPSEKFMQDELMKGAQEMYEAQKNAAPDDRLTVKEIYHKQLASFPMDIRLR
                     MRAAQQAYEVISSMGEALPTARAEAAADSISMNARIIRFTTLDLTEKSGGNIQVTEEE
                     IKAKHEKEQSIFEPAQRKSYESQKEFVKNRVLTEKRQLASAGLKQQLSQLGKDFKLED
                     IERITGKRASQVNIKLSQLKGVALPSGETVKLDGPEVLGALGGDGVKKIGPIQDGEAT
                     VYVEISGVQSVADAGLETRAQKIAEEKRNEAGRLYFELLLKKESERGRFKLKSEVKDT
                     GPVSHPDDSF"
     gene            3724..4188
                     /locus_tag="PKK76_08915"
     CDS             3724..4188
                     /locus_tag="PKK76_08915"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNL68636.1"
                     /translation="MKAEYVNPFLQGAVLVFRDLLQEEIIRGRTLVKKTPAPSHEIAI
                     LLNVTGSVHGEVIYSMNAESAYRIAEKLMPGTDRSSIEKEYRDVLGELGNMITGNALN
                     IFLNSQKDLDMSVPYVIDMRTQTLKVQDKTTLGLHLYSRLGMLELNIALQES"
     gene            complement(4310..>6055)
                     /locus_tag="PKK76_08920"
     CDS             complement(4310..>6055)
                     /locus_tag="PKK76_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNL68637.1"
                     /translation="ELAVPMAVRGEVIGVISVDSSGKNAFDRDHEHFLSIMSNLAAGI
                     FVNIKDNQLLKSRDRFHTVLHEISRIVSASLRLEDVFREIMTITEKAFKLHRSTLLLY
                     DRQQDVLAIVASVGFSKDELVDVTYAPGEGVTGSVFVNKKPVYIPSVSNEPGFLNRLK
                     TYGETGDMGFFCCPIFYGTEVVGVFSTFTRQQGLDAESMIEFLEILGSMISQSITIQR
                     LVKEETRVIASENIELKRELGSRYKFGSLIGKASSMIRLFEKVRIIADSRASVLLTGE
                     SGTGKELIASAVHYNSPRRDQPFVKINCAAIPENLLESELFGHRKGSFTGAIADKKGK
                     FETADGGTIFLDEIGELDLNLQSKLLRVLQEREIEPVGGRTRQVDIRIIAATNADLEA
                     GIADKKFRADLYYRLNVINLKIPALRERKDDILLLVNHFIEKYTKENGKSLTGISHAA
                     LKMLEDYEWPGNVRQLENAIERAVVLSQNKILDVDDFADTTLPFSAPPQNAGVPAVQA
                     VAVSSPGALELPDEEQFHHMDGHVYDSVIQEVEKRLILHALKKFKYTKTQAARYLGIN
                     RNTLDKKIRELSIDY"
BASE COUNT         1544 a         1551 c         1541 g         1419 t
ORIGIN      
        1 gtgcgatctc caatcactag attgtactga gtgcgcagat agcggacaat cgcttcgtcg
       61 aattcgtctc cgccaatgcg aatcgaatcc gaaatcacca taccaccgag agaaatgacc
      121 gcgatctcag tggttccacc accgatgtct acaatcatgc ttccagctgg ttcgtgaatc
      181 gggatgtccg ctccgatggc tgcagcgagc gcttcttcaa tcagaaagat ttctctggcg
      241 ccggcctgtt ctgcagattc gcgcaccgcg cgcttttcta cttctgtaat tccggatgga
      301 acaccgatca ccatgcgcgg ctttacgaaa gtagtgcgct tgtgaacttt ctgaatgaaa
      361 tagcgaatca tcttttccac ggtttcgaaa tccgcgatca caccatctcg catggggcga
      421 atggcgacga tgtcacctgg agtttttcca agcattctct ttgcttcgtt tccgacagcg
      481 agcaccttac ccgtagaaga ctgcaccgcg acgaccgagg gttccgaaag aacgataccc
      541 tgtcctttca catacaccag ggtatttgcc gtacccaggt cgatgcccat gtcattggaa
      601 aataaactga aaactcgatc aaacatcatg ataattgctg ttgtgggctg atttacaaag
      661 tgccttcatt ttggccattt caatttaaaa tgagggcaaa aaatgcccat cagggcgcct
      721 gagttcgcac cgggcgggta ggagacagac tggcgatgcg atcttcgagg aacttatcct
      781 tccctccgtt gagcttcagg gcttcctcaa aaaaaggacg tgccgcaatc gggccactct
      841 ggaccaggaa gatttcgcct tcaaggcggg ccgcgtccac cttccagatc gtttcagtat
      901 tgggattgta ttttacctgc ctggcgtaat ttagagcccg aatgtagtct ttcgcgttaa
      961 aacaggcatg gctgaggagc aggcgcgcct cgttcgcatc gagtgaatcc gggtccggtt
     1021 tggatccatc cacggggtcc acgagcatct ttctttctgt aaattcctgc tccagctcga
     1081 gggacttccc ctgcatggca aatagcgccc agcgcatcca gtccgtagtc tgctgggtga
     1141 gcggcgtcag ttcttccttc ttaatggcca gagtggtctg cagcaggttc gggtccgtgc
     1201 gacccgtcgc cattagctca ccaagaatga gagctgccct tacgcgcgaa gcgaaggcgg
     1261 gctttggatc aatcgccagg gatttgcgtg cgagggatgc ggtttcctgg gcgagcgcgc
     1321 tgatagagac cggagccaga ttcattcctt cgggtggatc aggaagatac cctctgccag
     1381 tcatgcgaag caaggaaggg ccattcaaag gcacacgaac cagaatctcg taatagttga
     1441 gaagactata gtaatagtaa acttctgcgc tggccggctt ttcgcgttcc atcaactgaa
     1501 cgattttgcg agtggattca actacatcca gaagttcggc cgggcttctt ccagccttct
     1561 tgtattcctg ctcaaagagt gcgagccgct tctgaattcg agtgtccgca ctgccgagaa
     1621 tctgccccga ggcaaacgca actacctggt ctccatagaa gacggccaca atggcgccgc
     1681 cgatcaggaa gagcgtgaga ctggcaatgg caaggggctt acgggacgat gtattggact
     1741 cgtagtttct cattcaggct gcattctctc gaatgaaagc cttcacgcga tccgattcat
     1801 ccgtcaagaa aatggcgatc ggcgattgcg tggagcctgg aaccagcgcc actccagaac
     1861 tcaaaaagac gatctgctcc aggtcgccgg tggaatatgc atgaactgaa ttgccggact
     1921 tgaagaagaa cccctctcct tcgcaaacaa gactcccttt gcccactctt ttgaaatcgc
     1981 tgaacatgcg cgggggcaag tcttctgtgc cacgcgagga aagaagagaa agattccgtg
     2041 cgtggtccaa aaacagagtg ccgaacacag caagttgccc cggatctttt cgcttcgccg
     2101 cagggcgaaa atcgctcacg gcaggcgcca caggagaaac ggcgcgcaca gcggcgtgcg
     2161 acagaatgga aggagacggt tctgcagaag gttccgcgac ggcttgagaa tgggaaacgg
     2221 gtgcatcttc tggatgaact ggcgccaggg gggcggggcg aacgctttcg tcgcgcaaat
     2281 ccggcgcacg ggttacgacg ggcatgatat ggcccggctc ttcctgatga cgctcagtcg
     2341 aaacagaaaa gaacagtaca gccaggctaa tgaggccgag cagaataaga cctatgccga
     2401 tgaaatacat gccctgcttg attatctcaa caatcggcct gactcttgag gaatattttg
     2461 tggcggaatt ggttccatga tgcgcgtatg gcccatgggt tcagtagata tcattaaaaa
     2521 attcggctcc gtgctgatca tcgtcattct cggagtcatg ctggccctta ctttttcaaa
     2581 aactcccttg agcacaatca ctgacattct ctcaggggcc gggagagtcg ggagctttga
     2641 cggggaggaa atccagcctc ggatctacct gatggcggaa gacaactgca gacagaacat
     2701 gagcgccttc ggccagatcc cggaatatat tctcaagaac tgcattgcca accagatccg
     2761 acagctctac gtgctgcctt tgcttgctca caggctgggt gtggctccat ctgagaaatt
     2821 catgcaagat gaactcatga aaggcgcgca ggaaatgtac gaggcgcaaa agaatgcagc
     2881 tccggatgat cgcctaacgg taaaggaaat ataccacaaa cagctcgcaa gtttcccgat
     2941 ggacatccgc cttcgcatgc gcgcggcaca gcaggcctac gaagtaattt catctatggg
     3001 agaggctttg cctacagcgc gtgcggaagc agccgcagat tctatctcga tgaatgcgcg
     3061 gatcattcgt ttcacgactc tggatctcac tgaaaaatca ggcgggaaca ttcaagtaac
     3121 ggaagaagaa atcaaagcaa agcacgagaa agagcaatcg atctttgaac cggcacaacg
     3181 caaatcctac gaaagccaga aggaatttgt gaaaaaccgc gttctcactg aaaagcgcca
     3241 gctagcaagt gcaggcctaa agcagcaatt gtctcagctg ggcaaagatt tcaaactgga
     3301 agatattgag cgaatcacgg ggaaacgtgc ctcgcaggtg aacatcaaac tgtcccagct
     3361 caagggagtg gctctgcctt ccggagagac cgtgaaactg gatggcccgg aagttctggg
     3421 ggcgctcggc ggagatggcg taaaaaaaat tggtccaatt caggacggag aagccacagt
     3481 ctatgtggaa atctcgggcg ttcaatccgt ggcggacgct ggactggaaa ccagggcaca
     3541 gaaaatcgcg gaagagaaaa ggaatgaggc agggcgcctg tactttgagc ttcttttgaa
     3601 gaaggaatca gagcgtggcc gtttcaaact caagtcagaa gtcaaagaca caggcccggt
     3661 ctctcatccg gatgattcat tctaaacact ggacggcaca aaacctgccg ataaaaaagg
     3721 ggagtgaagg ctgaatatgt gaatccattc cttcaaggag cggtcctggt tttcagggat
     3781 ctgcttcagg aagagatcat acgcggccga actctcgtta agaaaactcc agccccttct
     3841 cacgaaattg caatcctctt gaatgtgacc ggaagtgttc acggagaagt catctattct
     3901 atgaatgcgg aaagtgcata tcgcattgcg gagaagctaa tgcctggcac agatcgttcc
     3961 tctatagaaa aagaatacag ggacgtgctt ggcgaacttg gaaatatgat tacgggcaac
     4021 gcgctcaaca tcttcctcaa cagccagaag gacctggata tgagtgtgcc ttacgtaata
     4081 gatatgcgca ctcaaactct caaagtgcag gacaaaacta cactcggtct gcatttgtac
     4141 tccagactcg gaatgctgga gctcaacatt gcattgcaag agtcgtaata ttttttaagc
     4201 gcgatttgcg cgaaagaccg atgattaaaa ggtcattcgt gtaactccgc gggtccccgg
     4261 aacgggaccc gcatttttct aaacgactct ccgcctgagt tacacaaact cagtaatcta
     4321 tactcaactc acggatcttt ttatccagtg tgttgcgatt gattccgagg tagcgcgctg
     4381 cctgcgtctt ggtatatttg aacttcttga gagcgtgtag aatcaaccgc ttctccactt
     4441 cctgaatcac gctatcgtat acatgtccgt ccatatgatg aaactgctct tcatccggaa
     4501 gttcaagcgc gccaggagac gacacagcca ctgcctgaac tgcgggaact ccagcattct
     4561 gaggcggtgc agaaaagggt agagtcgtgt ccgcgaaatc atccacgtcc agtatcttgt
     4621 tctgcgaaag aacgactgca cgctcaattg cgttctccag ctggcgaaca ttccccggcc
     4681 attcgtaatc ttcgagcatc ttgagtgcag cgtgcgaaat gccagttaga gatttgccat
     4741 tttccttcgt gtatttctcg atgaagtgat tcacaagcaa aagaatgtcg tctttacgtt
     4801 cgcgaagcgc aggaatcttc aagttgatta cattcaaacg atagtagaga tcagcgcgaa
     4861 atttcttatc cgcaattccc gcttccagat cagcattcgt ggctgcgatg attcgaatgt
     4921 ctacctggcg agtgcggcct cccacaggtt cgatttctct ttcctggagc actctaagta
     4981 gcttgctctg cagattcaga tccagttcgc caatctcatc cagaaagatt gtgccgccgt
     5041 ctgctgtttc gaattttcct tttttgtccg caatcgcgcc ggtaaaagat cctttccgat
     5101 ggccgaacaa ctcgctttcc aaaaggttct cagggatcgc cgcgcagttg atttttacaa
     5161 atggctggtc tcttcgcgga ctattgtagt gaactgcgga tgcgatgagt tcctttccag
     5221 ttccggattc gccagtcaga agcaccgatg ctctagaatc cgcaatgatt cggactttct
     5281 caaacagtcg aatcatgctg gacgccttgc cgatcagact tccaaacttg tatctcgatc
     5341 caagctctcg ctttagctca atgttctcag atgcgatgac acgcgtttct tctttgacca
     5401 gacgttgaat cgttatggat tgggagatca tactcccgag aatttccaga aactcgatca
     5461 tagactccgc atcgagccct tgctgacgcg taaacgtaga gaaaactccc accacttcgg
     5521 ttccgtagaa aatcggacag cagaagaatc ccatgtcccc cgtttcgccg taggtcttca
     5581 gtcgattcag aaaacctggc tcattagaaa cacttggaat atacaccggt ttcttgttca
     5641 cgaaaacgga accggtgact ccttcgcctg gtgcgtaagt gacgtctacc agttcatctt
     5701 ttgaaaaacc aacactggcg acaatcgcca gaacatcctg ctgtctatca tagagcaaaa
     5761 gcgtactgcg atgaagcttg aacgcctttt ctgtgatcgt catgatttcg cggaacacat
     5821 cctcaagacg aagggacgca gatacaatgc gcgaaatctc atgaagcaca gtgtgaaatc
     5881 gatcgcgact cttcaacagt tggttgtctt tgatgtttac aaagattccg gccgccagat
     5941 tggacatgat ggagaggaag tgttcgtgat ctcgatcgaa tgcgtttttg ccgcttgagt
     6001 ctacagatat gactccaatg acttcgccgc gaacggccat agggacggcg agttc
//
LOCUS       DAORFF010000187         6027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_6997_length_6027_cov_4.313798, whole genome shotgun sequence.
ACCESSION   DAORFF010000187 DAORFF010000000
VERSION     DAORFF010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6997_length_6027_cov_4.313798"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PKK76_08925"
     CDS             <1..619
                     /locus_tag="PKK76_08925"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNL68638.1"
                     /translation="FELYYPHENRLKKAVFFYFLYLGGFWFSVPFASVVLLVLPWFSR
                     TWLVQRSVSASAMINGIPLRALTTIRLEGLFVIAAHAALIYFLDLNPARYAILMLAQG
                     VNWSCQQFLPHAHSPLDVIDGAYNLKVHRFYQALLLNFNLHLAHHQHPRVPWNYLDRF
                     DDPGRRRPGYFEAFARFWRGPQPISEFQAEVGVRRDSSVIRPSSM"
     gene            complement(562..1257)
                     /locus_tag="PKK76_08930"
     CDS             complement(562..1257)
                     /locus_tag="PKK76_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNL68639.1"
                     /translation="MNVLTATNLTKSFGTGESRATVVQNIHLSVRAGETVLISGPSGS
                     GKTTLLTMLGCMARPDGGEIQIQNVNVNSLSQAGLTSFRLRHIGFIFQAFRLIPSLTA
                     IENVQLTMNLAGLHSGSRKACMLLLEELGIAAKADKKSEVLSGGEKQRVAIARAIALN
                     PGLILADEPTGSLDGASGKAATAALVKTAKEKGAGLIIVSHDPRIELFADRTLHMEDG
                     RITEESRRTPTSA"
     gene            complement(1254..2360)
                     /locus_tag="PKK76_08935"
     CDS             complement(1254..2360)
                     /locus_tag="PKK76_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNL68640.1"
                     /translation="MIPIVRRFISGDLSKFLITLIGVGLTVALVLFLLAVYQGIKEAA
                     VVYIERSPAEVWVSQKNANNLLKVSSFLRADMQRELAATQDVREVSGILKVLSATTIR
                     GSSITMVLIGFEPQSEMGIPRTIIAGTSRIHNGEIILDSAFSKRHKIQLGDKLRIRDT
                     DFLVRGICDKTNATIAQFSFITLSDARDLIGVDGIVSFFLVGFNHGTPGQNAAVLGEK
                     FPNVSVFAKPAFINNNIVELNTGLLPVVRTISIFGTIISAVVITLMMYGSVVEKRELY
                     AIMKAIGASTWFLVGLVVKQALFASVGGALLGLCIALSVAPLLSKFVPEVALAFVYED
                     VLFVLAGSVLIGLVSAVAPLRSISRIYPAEAFRA"
     gene            complement(2618..2969)
                     /gene="ssrA"
                     /locus_tag="PKK76_08940"
     tmRNA           complement(2618..2969)
                     /gene="ssrA"
                     /locus_tag="PKK76_08940"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            3086..3463
                     /locus_tag="PKK76_08945"
     CDS             3086..3463
                     /locus_tag="PKK76_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68641.1"
                     /translation="MEQLISLVSGKVARTVFGLIIALFGVFHLMYANMMAGAVPVPGG
                     VIWVYFTGFCLIAGAVGIITNFKGQGRNAAFLTGVLVLVFAFGVQLLGVMRGGENAMM
                     YQTIFLKDIMIAMGAWVIAGTFR"
     gene            complement(3684..4130)
                     /locus_tag="PKK76_08950"
     CDS             complement(3684..4130)
                     /locus_tag="PKK76_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNL68642.1"
                     /translation="MIASIFSTPEDIALTVLRVTLAVVILPHGLQKLVGAFGGYGFKG
                     TMGYLTGTVGLPSVIAFLVIFIESFGAAGVLLGVGTRVAALGVAAVMLGAAWTAHRGN
                     GFFMNWTGAQKGEGFEFHILAIGLAIVLILRGGGAFSVDQILARLF"
     gene            4320..5675
                     /gene="hemN"
                     /locus_tag="PKK76_08955"
     CDS             4320..5675
                     /gene="hemN"
                     /locus_tag="PKK76_08955"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNL68643.1"
                     /translation="MEIELLKQYSKPGPRYTSYPAATAFTPDVGAEDYSMLLRDIDPA
                     RAISLYYHIPFCRNICYFCACNVIYTKNKEKAEPYIAALTREMDLVAEQTQARLKVEQ
                     LHWGGGTPSFLSHEQMNMLMAQIYRRYDFVPGAEISIELDPRETSPEQIGTLADLGFN
                     RASLGVQDFDPSVQTAVNRIQPEDITEQAYNHLRARGFRSINFDLIYGLPLQTVDTFA
                     KTMEKVVGFRPDRISIFNFAYVPWLKKHQLRMNKEDLPSTETRLQILSYAVDRLQKAG
                     YVHIGMDHFALPDDELTVSLRDGSLRRNFQGYSTRGGTELIGFGVTSIGEFNSGYAQN
                     TKSLVDYYRAIEAGRLPTERGLILTQDDRLRKAVIRQLITGLELKFETIEQAFDVRFE
                     EYFARELEAVGEFVDQGMLRFANRSLCVTEQGRFVIRNICMLFDAYLLGQNQGRYSNT
                     V"
     gene            5677..>6027
                     /locus_tag="PKK76_08960"
     CDS             5677..>6027
                     /locus_tag="PKK76_08960"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNL68644.1"
                     /translation="MRENPYRLFFPLSWLGAFALAFVWIPAVLIERHFLTIPGWNAYP
                     RDLHSTLAAGVFVLPAMTGFLLTAAPRMTGSSPASMIWVVALFFCEILIPPSVLLGGT
                     AIALLGACAILSAFL"
BASE COUNT         1422 a         1499 c         1538 g         1568 t
ORIGIN      
        1 ttttgaactc tactatcctc atgagaatcg actgaagaag gctgtatttt tctatttctt
       61 gtacctgggc ggattctggt tcagcgttcc gttcgcatca gtcgtgcttc tggttttgcc
      121 gtggttttct cgcacctggt tggttcaacg ttctgtttcc gcatctgcga tgatcaatgg
      181 aataccattg cgggctctca ctacgattcg ccttgaaggg ctctttgtga ttgccgcaca
      241 tgcagcattg atctactttc tagatttgaa tcccgcacgt tatgcgatat tgatgcttgc
      301 acagggagtg aactggtcct gtcagcagtt tctgcctcat gctcattccc ctctcgatgt
      361 cattgacggt gcgtacaatt tgaaggtgca ccggttttat caggcactgt tattgaattt
      421 caatcttcat ctggcccatc atcagcatcc gcgagtaccg tggaactacc tggatcgatt
      481 tgatgatccc ggtcgtcgta gaccgggcta ctttgaagcc tttgccaggt tctggcgtgg
      541 tccgcagccc atttctgaat ttcaggctga agtgggggtc cggcgtgatt cttctgtgat
      601 tcgaccatct tccatgtgaa gtgttcgatc tgcaaagagt tcgatgcgtg ggtcgtgact
      661 cacgatgatc aatcctgctc ccttttcttt tgcagtcttt acaagtgcgg ccgttgcggc
      721 tttccctgat gcgccatcca gactacccgt aggctcatct gccagaatca aacctggatt
      781 gagcgcaatc gcccgagcaa tagcgactct ttgtttctct cccccgctta gaacttcaga
      841 ttttttgtcc gcctttgcgg caataccaag ttcttccaga agcaacatgc atgcttttcg
      901 cgacccggaa tgcaggcccg ctagattcat tgtgagttgt acattttcaa ttgcggttag
      961 cgatggaatc aaacgaaatg cttggaaaat aaaaccaata tggcgcaggc gaaatgaagt
     1021 aaggccagct tgagacagcg agtttacatt tacattttga atctggattt ctcccccatc
     1081 aggtctggcc atgcatccga gcattgtgag aagtgttgtt tttccgctgc cgcttgggcc
     1141 ggatataagt accgtttcac cggcgcgaac gctgagatga atgttctgga caaccgtagc
     1201 gcgagattct cctgtcccaa agctctttgt aagattggtc gctgtcaaca cgttcatgcg
     1261 cggaatgcct ccgccggata aatgcgcgaa atacttcgga gcggagcaac cgcgctgacg
     1321 agtccaatca aaacggatcc agcgagcaca aagagaacat cctcatatac gaaagcgagg
     1381 gccacttctg gcacgaactt ggacaaaagc ggtgctacag acagagcaat gcacaggccg
     1441 agaagagctc ctccgacgga tgcgaacaaa gcctgcttca ccacaagtcc aaccagaaac
     1501 caggtagagg ctccgattgc tttcatgatc gcatatagtt cgcgcttttc aacgacgctc
     1561 ccgtacatca tgagtgtgat gactactgcg ctgatgatcg tcccaaaaat cgaaatcgtg
     1621 cgtactacgg gtaggagacc tgtgttgagc tcgacaatat tgttgtttat gaatgcgggt
     1681 tttgcaaata cagaaacgtt aggaaacttt tcgccaagca ctgcggcatt ttgccccggt
     1741 gttccatggt tgaatccaac caaaaagaaa ctcacgatgc catctacgcc aatcagatct
     1801 ctcgcgtctg aaagagtgat gaacgagaat tgagcgatcg ttgcgttggt tttatcacag
     1861 attcctctta caagaaaatc ggtgtcacga attcggagtt tgtctcccag ttgaattttg
     1921 tggcgctttg aaaacgcgct atctagaatg atttcaccgt tgtgaattcg tgaggttccg
     1981 gcaatgatcg tgcgagggat tcccatttcc gattgtggct cgaacccgat cagcaccatg
     2041 gtgatcgaac ttccccgaat cgtagtggca gagagcactt tcaagatgcc gctaacttcg
     2101 cgtacgtcct gggttgcagc taactcccgt tgcatgtctg ctcgcaggaa gcttgaaact
     2161 ttcaggagat tgttcgcatt tttttgactg acccagactt ctgctggact cctctcgata
     2221 tagacgacgg ccgcttcttt gatgccctgg tagactgcaa gcaggaacaa aaccagagcg
     2281 accgttaatc ccacaccgat cagagtgatg agaaacttac tcaaatcgcc tgaaatgaat
     2341 cttcgaacga ttgggatcaa atctgtttac ccatctgcat ccgccttctc ctgagagtgg
     2401 atgcggtgat ttttctgtct acgcattctg ttttcggcag cggtacacat tacgaactcg
     2461 aacgatagtt tctggtatcc gaaagctgag acgcggcggg acgattccga gcctccatac
     2521 gagcgggcag gggcacgaaa gcgaagccag gagggcgaag cgcaccacgg ctcgggcacg
     2581 gacgctcgcg ccgtggtggc gtgccccctg cccgttccta tggagacggc gggagtcgaa
     2641 cccgcgtcct acggtgcgaa gcatgtgctt ctacgtgcgt atccagcaat taataatccc
     2701 ggccgatgcc tgctggaggg gcctgtccga gatgctcaat ctgtattagt ttgccgtacc
     2761 gcagattgaa ccggtccgac tcatcctccc aaagtttgac actcttctac aatccggaag
     2821 gagggactgc agggagcgct ggtcgcttaa attaagcggc tagagccatt tcgttgttgg
     2881 cagttacaag tttgaagttt ttaagagctc ttcgactctg cacgcaacac acaccccgaa
     2941 aacacagcag tcgaagccaa ttcgtccccg tggtgtatcg tatgcattat aaggcattca
     3001 tggaagcaaa gcaagcgaaa ttttcgcagt ttgctttgac gcgaaacaca taacagagta
     3061 tccttcaacc aaaaggagac attgtatgga acagttgatt tctctggtgt ctggcaaagt
     3121 ggcgcgtaca gttttcggac tgatcatcgc tttgttcggc gtctttcact tgatgtacgc
     3181 gaacatgatg gcgggcgcag tcccggttcc gggcggagtg atctgggtgt acttcacagg
     3241 gttttgtctg attgctggcg cggtgggtat catcacgaac tttaagggac agggccgcaa
     3301 tgcagcgttc ctgaccggtg tgctcgtgct ggtgtttgcg tttggcgttc aactactcgg
     3361 tgttatgcga ggcggggaaa atgcgatgat gtatcagacc atttttctca aagacatcat
     3421 gatcgccatg ggagcgtggg tcatcgcggg aaccttccgc taggacgtgc gtgatctgtc
     3481 gccctgattt tgtctctaga atcagggcga ttcctatttc ccgactggta cattggatat
     3541 cgtcgcaaga ctcgcaaagc aagcataccg gagcatcgta tgcaattgat ttggatcgtc
     3601 aagcgatgcc gtaaggtgga acttagaata ggatgcgccg gaacaagccg gcgcgaaata
     3661 cttgttaggt gaatatcttt gttttagaag agcctggcca ggatctgatc tacgctgaag
     3721 gcccctccgc ctcgaaggat gagcacgata gcaagtccaa tcgcaaggat atgaaattca
     3781 aatccttctc ctttttgtgc tcccgtccag ttcatgaaga aaccatttcc tctatgcgcg
     3841 gtccaggcag ctccaagcat tacagcggct acaccgagag ctgcgaccct tgtgccaaca
     3901 ccgaggagca ctccggccgc tccaaatgat tcgatgaaaa tgacgagaaa tgcgatcaca
     3961 gagggcagtc caactgtgcc cgtcagatag cccatggttc ctttgaaccc gtaaccacca
     4021 aaagcaccta ctagtttctg caagccatgg ggcaaaatca caacggccag agtgacgcgc
     4081 aggacagtta gagcgatgtc ctccggcgtc gaaaagattg aagcgattat cataatgtaa
     4141 accttccttt tatgaagctc aaaataatgc tttaatgttc aatcgtcaag gtttaatggg
     4201 atgaaatatt ccttttttct ttggatttga tgtcagtcaa ttgccgcgcc gctagaatca
     4261 actttcaggt atgagaaata tcatagcagg aactgccgcc gagggggcat actagcgaca
     4321 tggaaattga actgttgaaa caatacagta aaccgggacc gcgttataca agctatcctg
     4381 cggccactgc tttcacgccc gacgtggggg cagaggatta ctcgatgctt ctacgtgaca
     4441 ttgacccggc ccgcgcgatt tctctttact atcacattcc tttctgtcgc aatatctgtt
     4501 acttctgtgc ctgcaacgtc atctacacga agaacaagga aaaggcagag ccatacatcg
     4561 cggctctcac tcgcgagatg gaccttgttg cggagcaaac ccaggcacga ctgaaggtag
     4621 aacaacttca ctggggtggt ggaactccgt cgtttttgtc gcacgagcag atgaacatgc
     4681 tgatggcgca gatctacagg cgatacgatt tcgttccggg cgcggagatc agcattgaac
     4741 tggaccctcg cgaaacatca ccagaacaaa tcggcacgct ggccgatctc ggattcaatc
     4801 gcgcctcact gggcgttcag gattttgatc cttccgtaca aaccgcagtc aatcgaattc
     4861 aacctgagga tataacggag caagcataca accatctgcg cgcacgcgga tttagatcca
     4921 tcaacttcga tctcatctat gggcttcccc ttcaaaccgt cgacacgttt gcaaagacga
     4981 tggagaaggt agttggattt cggccggatc gaatttcaat ttttaacttc gcatatgttc
     5041 cgtggctgaa gaagcatcag ctccgcatga acaaagaaga cctcccatct acagagactc
     5101 gcctgcagat tctttcgtat gctgtagatc gtcttcaaaa agcaggatac gttcacattg
     5161 gcatggacca tttcgcactg ccggatgatg aattgaccgt gtccctcagg gatggcagcc
     5221 tgcgaagaaa ctttcagggc tattctactc gaggcggcac agagctcatt gggtttgggg
     5281 tcacctccat cggagagttt aacagtggtt atgcgcagaa cacgaaaagt cttgtggact
     5341 actatcgtgc cattgaggct ggccgactac caacggagag aggtttgatt ctaactcagg
     5401 atgatcgcct gcgcaaagcc gtgattcgcc agttgattac gggccttgag ctgaagttcg
     5461 agacgataga acaggctttt gacgttcgtt ttgaagaata ttttgccaga gaactggaag
     5521 cggttggcga atttgtagat cagggaatgc tcaggttcgc gaatcgatct ttgtgtgtga
     5581 ccgaacaggg tcgatttgtg attcgcaata tttgcatgct gttcgacgcc tacttgttgg
     5641 gccaaaacca gggtcgatat agcaacacgg tttagaatgc gagaaaatcc ataccgtctg
     5701 ttctttccat tgtcctggct gggagcattt gccctggctt ttgtatggat tccggcagtt
     5761 ttgattgagc ggcacttcct gacgattcca gggtggaatg catatccccg ggatttgcac
     5821 agtactcttg ccgctggtgt gttcgtattg cccgcgatga cagggttcct gcttacagcg
     5881 gccccgagga tgacaggctc ttcgcccgca tccatgatct gggtcgttgc cctgttcttt
     5941 tgtgaaatat tgattccacc ttcggtcctg ttaggtggaa ctgcgatcgc gcttctggga
     6001 gcttgcgcga tattgtctgc gtttttg
//
LOCUS       DAORFF010000188         6021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7017_length_6021_cov_3.490278, whole genome shotgun sequence.
ACCESSION   DAORFF010000188 DAORFF010000000
VERSION     DAORFF010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7017_length_6021_cov_3.490278"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PKK76_08965"
     CDS             <1..222
                     /locus_tag="PKK76_08965"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNL68645.1"
                     /translation="FQCKAFGGGNMFRERVDNVEVIGEKNIRAVEEMTQSGKLVVLSS
                     DLGGAFYRRVQFNVWSGEVWVKRKATEQA"
     gene            219..1451
                     /locus_tag="PKK76_08970"
     CDS             219..1451
                     /locus_tag="PKK76_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL68646.1"
                     /translation="MKSRVLIAEDDAVNGRLLESILGGSEFEATLVSSGEEALRASKE
                     NKYDLLVTDLHMPGISGIELVEKLRAEGNPVQAIILTGEEDVSTVVRAMRVGIFDYVP
                     KPVQPEEFVLRVKDAIEAGRLVQAAERATRDRDRRMERQVALSVWKEDVLGRDRDRYN
                     KTLFDNLRMSFNQGIGFGGLVTLPSLIADTSKIVDGNYLVGTDLMDLLIENARMAESA
                     MRRFEEIDQLIRGEIDLREASIAEVHTLVCRVADSLHSLLDIKSQSVVVSEPPRQSGI
                     VRISERLLTDAVRELLINAMKFSKPSSQIFVLFRRTSSHLHCSFVNTPGENQFGSRGV
                     PEEHERLIFEPFYRIVRTVDERFGTLDYGLGLSVVEKIIQGHQGRIRAGNIHDFFSKG
                     AEGESAANFELQLPLIDT"
     gene            1458..1787
                     /locus_tag="PKK76_08975"
     CDS             1458..1787
                     /locus_tag="PKK76_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNL68647.1"
                     /translation="MVIDSQKKEEIVNIGGEGFFLEMLDKFIAQAIEILGQIRSAKAA
                     GNADQAKSLLHKLKGSSLTIGADDFARHVLRAYEKAQAGVMVEDEDLGLMDACLVELQ
                     AHRKTLQ"
     gene            complement(1788..2279)
                     /locus_tag="PKK76_08980"
     CDS             complement(1788..2279)
                     /locus_tag="PKK76_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68648.1"
                     /translation="MNRRTILFWLLLAPAPLCALTPDSTSILFTDAVVPGFGSFYTGR
                     PVAGSMIAGTRVGTAYLAWYFKQREREYRSAENAARIAEFYFGPGYRFKNPYGSGYYS
                     ASEFHRMAGRREFYSHLAILFHAGITVASLFATESFLSLDAEQNGPVFPEPRVRVSLS
                     VPF"
     gene            complement(2276..2686)
                     /locus_tag="PKK76_08985"
     CDS             complement(2276..2686)
                     /locus_tag="PKK76_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68649.1"
                     /translation="MKRCLFFLLLALGCDRAHTTPPGQFLLPLLRSGGARPTTPTNLR
                     ARYAASISALTMDWVGSIDPDTGLPNVVYSIYAYELPPREYYRRQDILATTTLTSYYQ
                     SADHFTGSLYFVVTAFDGGAESLPSNVAQLESSL"
     gene            complement(2683..3090)
                     /locus_tag="PKK76_08990"
     CDS             complement(2683..3090)
                     /locus_tag="PKK76_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68650.1"
                     /translation="MPKIRHAYNQFSARLRNCIVLLFLLPTCRTAYPFYVERPAGAIK
                     VKLYLLESDYRGERNQKAVAMALSGKWAEALGTWTEMKNRKLAIDCGLFSNYAISLYM
                     NGEPAWAAMEQAVRMCPDDNEVRHNFRMLSVLQ"
     gene            3084..3326
                     /locus_tag="PKK76_08995"
     CDS             3084..3326
                     /locus_tag="PKK76_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68651.1"
                     /translation="MALLLLVACQKDPDTTPQKLATYILVCGAGVQACYTNCGNTYDA
                     NRNGTIDASEFYNFKTCTGVCDANCDVSFLYTALSK"
     gene            complement(3327..4943)
                     /locus_tag="PKK76_09000"
     CDS             complement(3327..4943)
                     /locus_tag="PKK76_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68652.1"
                     /translation="MNKAQAVSFLSIVLFAATAALAPTLGIWALPDPPRWLVLLLLAF
                     AFVPLLVDRLLPENRMILVRFPPLLIAVFSGLAAVLMWQFRQRLFLPAPNGNGDSHFL
                     IEQIPVYAELFGARLSFDEILALFVHSKFYLLCNKFGVGIIDSYAILSCGAGILYTAT
                     ALFYLNRRPLLSWLAGASVLLLTPAISIFFGYVEHYDAVSLILFVVAVMCLHLMDKDV
                     STPAWIAIGLLSALGALFHMMGGLALFPLLYFVWFKTGNRHDFIRAALKAGIPAITFL
                     AFAWIYFLLLADNRINLRESFFVKPPIYPLREFFSGKHIFDSINLILLGMPFAGGALL
                     YFLFHREMFRTTISRPDVRFLLIGLAAFFTASLFINPLLSFPSDWDLFTMFQVFGNLT
                     LAAIFAHIHSKENKLTAIALCALIICSTSTVLWIHRNHSTTPESELTVKRAIESSQGF
                     LEQIKNDPVYAKTPPQRRKTSVQARLFLYGSERKLLNKPGHKELLAEVQEESRRFDEF
                     ILLPEAEYQKAYATVWPRLTDLNRRLESIE"
     gene            complement(4936..5601)
                     /locus_tag="PKK76_09005"
     CDS             complement(4936..5601)
                     /locus_tag="PKK76_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68653.1"
                     /translation="MDRELSVERPELREKLLSIARLQYAVHPGIITPDESGKISPQRL
                     FKNPDSFHVLELGAGFGELCVEYMRSNPDHEYTAFEIKWDRIRNLLKHADADRLHPRV
                     VPVDFDWFFESMLPAHSFDRIIIYFPDPWPKRRHWKHRLIHKGFPDRLSPIMRPGADI
                     YLATDYGPYARRMLSAFRKSALKPAFPFPHYVRENPLPTRTHFEKLMSQKRKPYFMVW
                     KNE"
     gene            5659..>6021
                     /locus_tag="PKK76_09010"
     CDS             5659..>6021
                     /locus_tag="PKK76_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL68654.1"
                     /translation="MDVKFWGVRSDVPSSLSTEEMQSRVKQAVLFAHEAWKVNPDLPP
                     DEVIASLPVHIKRLIGGETRCLEITAGTDRIIIDVGTGARRLGYDLMMRGVKGEFNVI
                     LTSTRWDHVQGWPFFVPVY"
BASE COUNT         1577 a         1419 c         1649 g         1376 t
ORIGIN      
        1 tttcaatgca aagcctttgg cggcggaaac atgtttcgcg aaagagtaga caacgtggaa
       61 gtgatcggcg aaaaaaacat tcgtgcggta gaagaaatga cccagtcggg gaaactcgta
      121 gttctttcct cggacctggg cggagcgttc tatcgcagag tccagttcaa tgtctggagc
      181 ggagaggtct gggtgaaacg taaagcaacg gagcaggctt gaaatcgcgc gttctgatcg
      241 cggaagatga tgcagtcaac ggcaggcttc tggaatcgat cctcggcgga agtgaatttg
      301 aggcaactct agtctcaagc ggagaagagg ctcttcgcgc gagcaaagaa aacaaatacg
      361 atttgctggt gacagacctt cacatgcctg gaatcagcgg cattgaactc gtggagaaac
      421 ttcgagcaga gggaaatcca gtccaggcca tcatcctgac cggcgaagag gacgtatcca
      481 cggtcgtgcg cgcgatgcga gtcggaatct ttgactatgt tcccaagccc gtgcagccgg
      541 aggagtttgt acttcgcgta aaggatgcca ttgaagccgg aaggctggtg caggcggcgg
      601 agcgcgccac gcgagaccgg gaccgcagga tggaacggca ggttgcactt agcgtctgga
      661 aagaagacgt gctcggtcgc gatcgggatc gatacaacaa gactctgttt gataaccttc
      721 gcatgagttt caatcaggga attggatttg gcggtcttgt aacactccct tctctgattg
      781 cggacacttc aaagatcgta gacggcaatt atctcgtggg tacagatctc atggatctgt
      841 tgatcgaaaa cgcgcgcatg gcagaatctg caatgcggcg atttgaagag atcgatcagc
      901 tgatccgagg agaaatcgat ctaagggaag cttcgattgc agaagtgcat acactcgttt
      961 gcagagtggc agactcattg cacagccttc tggatatcaa gagtcagagc gttgtggtga
     1021 gcgagccgcc gcgccagagt gggatcgtgc gaatttcaga acgacttctc acggatgcgg
     1081 tccgggaact cttgatcaat gccatgaagt tttcaaagcc ttcctcccaa atctttgtac
     1141 tatttcgcag aacatcgagc catctgcact gctctttcgt aaacactcca ggtgagaatc
     1201 aatttggatc cagaggtgtg cccgaggaac atgagagatt gatcttcgaa cctttctata
     1261 gaatcgtccg cactgtggac gagcgattcg ggacgctgga ctatggtctt ggtctttctg
     1321 tcgtggaaaa aatcattcag ggacatcagg gaagaattcg agcgggcaac attcacgatt
     1381 tcttcagcaa gggtgcggag ggtgagtcag cggccaattt tgagttgcag ctcccgctga
     1441 tcgacacata gtcgctcgtg gtcattgatt cccagaaaaa ggaagaaatt gtaaacatcg
     1501 gaggggaagg attcttcctc gagatgctgg acaaattcat agcgcaggca attgaaatcc
     1561 tgggtcaaat tcggtctgcg aaagcggccg gaaatgcgga ccaggcaaaa agtctgctcc
     1621 acaaattaaa gggatcgagt ctgaccattg gcgcggatga ttttgcacgc cacgttcttc
     1681 gcgcgtatga aaaagctcag gccggagtga tggtcgaaga tgaagatctc ggccttatgg
     1741 acgcttgcct ggtagaactc caggcacacc gcaagacact ccaatagtta aaaaggaacg
     1801 gacagagata ctcgaactcg cggttcggga aacaccggac cgttttgctc cgcatcgagg
     1861 gaaagaaagc tctctgtcgc aaacagactg gccacggtaa tcccagcatg aaacagaatc
     1921 gccaggtgag agtagaattc tcttctgcct gccatcctgt gaaactccga ggcactgtag
     1981 tagccggaac catacggatt cttgaatcga tagcccgggc caaagtagaa ctccgcgatc
     2041 cgcgctgcgt tttctgcgga tctgtactcc ctttctcttt gcttgaagta ccatgccaga
     2101 taggctgtgc cgactctcgt gccggcgatc atggagccag ccaccggtct gcctgtgtag
     2161 aaagatccga agcctggcac aactgcgtct gtgaacagaa tggatgtgga atccggtgta
     2221 agggcgcaca ggggtgctgg ggccagcagc agccagaata ggatggtgcg cctgttcaaa
     2281 gagatgactc cagttgtgcc acgttagacg gcaatgactc cgcacctccg tcaaatgctg
     2341 tgactacaaa atacagtgat cctgtaaaat gatccgctga ttgatagtaa gatgtgagcg
     2401 tagtggtcgc aagaatgtcc tggcgtctgt agtattcgcg cggaggaagt tcatacgcgt
     2461 aaatggaata gactacgttt ggaagccctg tgtcaggatc tatgcttcca acccaatcca
     2521 tcgtcaaagc ggaaatggac gcagcgtatc gcgcacgtag gttcgtggga gtggtgggcc
     2581 ttgctcctcc actgcgcaac agaggaagaa gaaattgtcc aggcggcgta gtgtgtgcac
     2641 gatcgcagcc cagagccagg agaaggaaga acaaacaccg tttcattgaa gtacgctcag
     2701 catacgaaaa ttgtgtctta cttcattgtc atccggacac atacgaactg cctgttccat
     2761 ggcggcccag gctggttcgc cgttcatata aagggaaata gcgtagttgg agaagaggcc
     2821 acagtcaatg gctagcttcc tgtttttcat ttcagtccac gtgcccagcg cttctgccca
     2881 tttcccggaa agtgccattg caactgcctt ctggtttctt tctcccctgt agtctgattc
     2941 gagcaggtag agcttcactt tgatcgcgcc ggccggccgt tccacgtaga aagggtaggc
     3001 agttctgcag gtaggaagaa gaaacaaaag gactatgcag tttctgagcc gagccgaaaa
     3061 ctggttgtat gcgtgtcgta tttttggcat tgctcttgct ggttgcctgc cagaaggatc
     3121 ctgacacaac tcctcagaag ttagcaacct atattcttgt ttgcggcgcg ggcgttcagg
     3181 catgctatac caactgcggc aatacttacg acgcgaatcg aaacggaacc atcgacgcat
     3241 ctgagtttta caacttcaag acctgcaccg gcgtgtgcga cgccaactgc gacgtgagct
     3301 tcctctacac cgctctctca aagtaatcat tctatcgatt ccagtctgcg attcagatcc
     3361 gtcagtctgg gccagactgt ggcgtacgcc ttctggtatt ctgcttccgg aagcaatatg
     3421 aactcatcaa agcgtctgga ttcttcctgg acttccgcga gaagctcttt gtggccgggc
     3481 ttgttcagca gttttctttc agatccgtaa agaaagagtc tcgcttgaac agatgtcttt
     3541 cttctctgcg gaggtgtctt cgcatagacc ggatcattct tgatttgctc aaggaacccc
     3601 tgggaacttt caatggctct ttttacggtc aactcgcttt ccggagttgt tgaatgattg
     3661 cgatgaatcc agaggacggt gcttgtgctg caaatgatca gtgcgcaaag agcgatcgcc
     3721 gttagtttat tttctttaga atgaatatgc gcaaaaatcg cggccagagt gagattccca
     3781 aagacctgaa acatggtgaa aagatcccag tcagatggaa agctaagcag tgggttgatg
     3841 aacagcgacg cggtgaagaa tgcggccaga ccaatgagta agaatcgaac gtctggtctt
     3901 gagatcgtag tgcgaaacat ttctctgtga aacaagaaat acagaagggc acctccggca
     3961 aatggcatac caagcaagat caagttgatc gagtcgaaga tgtgcttgcc ggaaaagaat
     4021 tcacgtaatg gatagatggg cggctttacg aaaaacgatt cgcgcagatt gatgcgattg
     4081 tcggccagga gtagaaagta gatccaggca aaggccagaa aggtgatggc gggaattcct
     4141 gctttcaggg ccgcgcgaat gaaatcgtgc ctgtttccag tcttgaacca tacaaaatag
     4201 agaagcggaa aaagtgcaag ccctcccatc atgtgaaaga gcgcaccaag cgcggacaga
     4261 aggccaatcg caatccaggc aggagtagaa acatctttgt ccatgagatg caaacacatc
     4321 accgctacca cgaatagaat caaggacaca gcgtcgtagt gttccacata accaaagaag
     4381 atggaaatgg caggtgtaag taacagaaca gatgcaccgg ccaaccacga aagcaaaggt
     4441 cgtctgttta gataaaagag agcagtggca gtgtatagaa ttcccgcccc acagctcaag
     4501 atcgcatagc tatcaatgat gcccacgccg aatttgttgc agagaagata gaatttgctg
     4561 tgcacgaaca aagcaaggat ttcatcgaag ctcagtcttg cgccgaataa ctctgcgtag
     4621 acggggattt gttcaatgag gaagtgactg tctccatttc cgtttggagc aggcaaaaac
     4681 agtctctgtc tgaattgcca catcaagacc gcagccagtc cggaaaaaac agcaatgagc
     4741 agaggaggga atcgaaccag aatcattcga ttttctggaa gcagtctatc tacaagcaaa
     4801 gggacaaaag caaaggcaag aagcaaaagg accagccagc gcggagggtc cggcagtgcc
     4861 cagattccta gagtcggggc cagggcagct gtggctgcga agagcacaat ggaaaggaag
     4921 gaaaccgctt gagccttatt catttttcca taccatgaag tagggtttgc gtttttgcga
     4981 catcagcttt tcgaaatgcg tgcgagtggg cagggggttc tcccttacgt aatgaggaaa
     5041 cggaaaagca ggcttgagag cggacttgcg aaaggcactc agcatccgcc tggcatatgg
     5101 accgtaatcc gtcgccagat agatgtccgc tccaggccgc atgatcggac tgagtctatc
     5161 cgggaagcct ttgtggatga gtctgtgttt ccagtgtctc cttttgggcc agggatctgg
     5221 aaaatagatg atgattcgat cgaatgaatg cgcaggaagc atcgattcaa aaaaccagtc
     5281 gaagtcaacg ggaaccacac gcgggtgaag ccggtcagca tctgcgtgct tgagtagatt
     5341 gcgaatccga tcccacttga tttcaaacgc agtgtactca tgatctggat tcgatcgcat
     5401 gtattcgacg caaagctctc caaatcccgc gcccagttct agaacgtgga agctatccgg
     5461 attcttgaac agtctttgcg gactgatctt tccggattca tctggcgtga tgataccagg
     5521 gtgaacagca tattgcaatc gggcgatcga tagaagtttt tccctcagct ctggcctctc
     5581 tactgatagc tctctatcca tccggagtcc agaaaatctt ttggtttaca gggagccatc
     5641 acgttcctgg ataccagcat ggacgtgaaa ttctggggtg tgaggtcgga tgttccttcc
     5701 tcgctttcga cagaggaaat gcagtccaga gtcaagcagg ctgttctgtt cgcgcacgaa
     5761 gcctggaagg taaaccccga tcttccgccg gatgaagtca ttgcgtctct tccggttcat
     5821 atcaaacgtc tcataggtgg agaaacgcgc tgcctcgaaa tcacggcggg aacagaccgc
     5881 atcatcatag atgtaggcac aggcgctcgc aggcttggct atgatttgat gatgcgcgga
     5941 gtgaagggcg aattcaatgt aatcctgaca tccactcgtt gggatcacgt gcagggttgg
     6001 cctttctttg ttcccgttta t
//
LOCUS       DAORFF010000189         6007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7039_length_6007_cov_3.382560, whole genome shotgun sequence.
ACCESSION   DAORFF010000189 DAORFF010000000
VERSION     DAORFF010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7039_length_6007_cov_3.382560"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..539
                     /locus_tag="PKK76_09015"
     CDS             162..539
                     /locus_tag="PKK76_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system-associated lipoprotein"
                     /protein_id="HNL68655.1"
                     /translation="MHRSVLIVLLLISACTSAVIPKEKVRQLNREFEGRTYIAIVDIK
                     PEFSDSSDAKTEIIFHKNDRLRLVAENGEEWIRVKAHIVSDRPEQAIGRAIVFILRDD
                     LKKDADPLSEVRARLDKLVSVKR"
     gene            545..1627
                     /locus_tag="PKK76_09020"
     CDS             545..1627
                     /locus_tag="PKK76_09020"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNL68656.1"
                     /translation="MKIRQYRLDNPIMRLAVFLLVLSTALYAETETDFSQEARPMPAL
                     VPQHAESDEIDQEIKRYFEHATSGMDTDQKRLDRIFMNNEAQTRPGEDEGSFTFHELG
                     NKDETYRVKAKDTIYSISKKFGVKPQDIIKQNPDLETRALYIDEEIVIRSAKKPGPVP
                     GTSVQPTYLVQKGDNLSLIARKYRTTPAVLMKINGLGPASALKTGMSLRLPGPPLLYR
                     SMFAWPVQAPITSYFGRRYNPFLGGFPQFHKGLDLGAQLGTQFHAARDGIVIYSGRME
                     GYGNVIFVRHVGGLVTVYGHNKLNLVKQGDIVRQGQVIGQVGRTGSATGPHLHFEVRK
                     QELALNPLVALGWKEVVPPGQVAEAR"
     gene            1640..2488
                     /locus_tag="PKK76_09025"
     CDS             1640..2488
                     /locus_tag="PKK76_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68657.1"
                     /translation="MLEIVHRLQKNVFLVTLGAAVFLGFSLAYLLTNIIPFFISDSGS
                     VQIASSSDRRPVEVDVVRPLQDFEGVITGNFIRDSGDAGTKGPGTETGEGEITLLGVL
                     SGPPQFARAMVQIVGKPEIETYRLGEAVAGFKIIQILGNSVVVERGSNNLRIYVGEKS
                     GEAAAAPQAGNAPSAQGATKINITRDRLLASTKNPEMIYQNKFAPVTRDGKVLGLKLL
                     FVPQGNFLYELGARSGDIIRRFNGEPLENQDRMIQMWQSLQSANRITIEVERGGKVIP
                     FEILIQ"
     gene            2502..4325
                     /gene="gspD"
                     /locus_tag="PKK76_09030"
     CDS             2502..4325
                     /gene="gspD"
                     /locus_tag="PKK76_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HNL68658.1"
                     /translation="MKQGCKSRTIARILGLAALFAVSHALAPQTPPPTSTRDSCTPTK
                     NTFCVSYRDIEIADFLKLMAQIAKKNVLVDETIKGKITILSHSPIPVNRALDFMKQVL
                     ELRGLTVLEEPNLVKVVPMDVASSASIPDPNRPTDQDTTLTSQILRVPSSVNINDLAN
                     VLKSLAGKNTVVAPYKAANTIIITGYAPNVRRVLSLANELIPRLDVPGGTNVETDTVH
                     IYRVKNMPAESLAQTLTRLDNPQAAATPAQPANPAQPGQPPVPVPVPVSVPETPGKPA
                     GKIRAVSHKESNSIIVTASDAEWREIEQIIRQLDMFRQQILLEVLIAEVSSTDLNDFG
                     IDWRYQGTNSGHSQFNTGLAAEGKLVDPDTGKITGNNTLSGFSLGFLKKGGELLGIFN
                     ANMNNQNFNVLSAPQVLTLDNQEAEINVGQDVPVRTQERTSGGGSSEATVNSFEYRPS
                     GIKLKFTPHVNAEGMISIDLFSEVTNIEGASRSAGNPTFNKRNVKTYITVGNKQTIVI
                     GGLVGSEKQEAVSKVPLLGDIPVLGFLFRRTTISDKRTNLMVFITPHILAEKAEADRL
                     TDFKREEQSRAYRQRHNNIRLWPQKEVPLGRDDDLDKIEAE"
     gene            4322..5989
                     /gene="gspE"
                     /locus_tag="PKK76_09035"
     CDS             4322..5989
                     /gene="gspE"
                     /locus_tag="PKK76_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HNL68659.1"
                     /translation="MKKQLGEILLQEGVITKEDLQEVLKIQKKSPLKLGQILQKKGLA
                     DEDQILKTLANQFGYEYESRLTFKFDDAFKKIPVNLLQKAKMVPVSRTGKSIKVALSD
                     PADLHPADDLRNILADFRVSFVLAPEAEVLKIIHGQFDQAANAAKEVMEGISEDEFQD
                     MDALGDDTLDLANEAPIIRMVNAILTQGVTERASDIHIEPFEKSVEVRYRVDGILHKR
                     LSPPKVIHAGIVSRIKIMANLNIAENRLPQDGRIKIKLSGKDVDIRVSTIPTRHGERV
                     VMRLLNKSDVRFTLDNMGLPKPMLETTQRLLKEPNGIVLVTGPTGSGKSTTLYAFLQE
                     MNQESRNILTAEDPVEYEIEGISQMQMQEKIGLTFATALRAMLRQDPDVIMVGEIRDQ
                     ETARIAIQAALTGHLVLSTLHTNDAPSAVTRLVDMGIEPYLITSTVRAVLAQRLSRVL
                     CPSCKEPFRASARDLASLGLSGAEAGKIYKHVGCENCVGTGYRGRTGLYELMLMDEDV
                     QRIVIRGGDSEAVKKVALEKGMQTLQTYGKNKVLDGVTTIEEVLRVT"
BASE COUNT         1640 a         1545 c         1510 g         1312 t
ORIGIN      
        1 gcaggcccga atcctttcta gagttccggt caatccagcc atagaccagt gcggaattcc
       61 gcgacgtgca gaagcttcca cggaagcgac agcggaggcc ggcccggatg tcagggtaag
      121 ggtgcttgca tatttcatca cctttcgatg gtatggcaac tatgcatcgt tccgtcctga
      181 ttgttctact ccttatcagt gcttgcacct ctgctgtgat tccaaaggaa aaagtccggc
      241 agctcaacag agaattcgag ggtcgcacct acattgcgat agtagacata aagcctgagt
      301 tttcggacag ttctgatgcg aagactgaga tcattttcca taagaacgat cgcctgcgcc
      361 tggtcgcaga aaacggcgaa gagtggatcc gggtcaaggc acacatcgtt tctgaccggc
      421 cagagcaggc cattggccgc gccattgtat tcattttgcg ggatgatttg aaaaaggacg
      481 ccgatcctct ctccgaggtg cgggcccgac tcgataaact ggtgagcgta aaacgatgaa
      541 acatgtgaag atcagacaat accggctcga taatccaatc atgcgtctgg ctgtgtttct
      601 gcttgtgctc tccacagcgc tctatgccga aactgaaacc gatttcagtc aggaagcccg
      661 tcctatgccg gctctcgtgc cgcaacatgc ggaatccgac gaaattgatc aagaaatcaa
      721 acgctacttt gaacacgcaa cttccgggat ggacacagac cagaagcgcc tggaccgaat
      781 cttcatgaac aatgaagctc aaacaagacc aggcgaagac gaaggctcat ttacatttca
      841 cgaattgggc aataaagacg aaacctatcg cgtcaaagcg aaagatacta tctatagcat
      901 ttcgaagaag ttcggcgtca aaccacagga catcatcaag cagaatccag atctagaaac
      961 gcgtgcactt tacattgacg aagaaatcgt gatccgatct gcgaaaaagc caggtccagt
     1021 gcccggaaca tccgtgcagc caacctacct cgttcagaaa ggggacaatc tctccctcat
     1081 tgcacgcaag tacagaacca ctcccgcagt tctcatgaaa ataaatggac tgggacctgc
     1141 gagtgctctt aagactggaa tgagtttgag acttcctggt cctcctctac tttaccgctc
     1201 gatgttcgcc tggcctgtgc aggctccgat cacttcctat tttggaagac gttacaatcc
     1261 ttttcttggc gggtttcctc aatttcataa aggactcgat ctgggtgcgc aactgggcac
     1321 gcagttccac gcagcgcgcg acggaatcgt catttactca ggtagaatgg aagggtacgg
     1381 aaacgttatc ttcgttcgcc acgtgggtgg acttgtaacc gtctatggtc acaacaagct
     1441 gaacctggtc aaacaaggag acattgtgcg ccagggtcag gtgatcgggc aagtagggcg
     1501 caccggatct gcaaccggac cgcatttaca ttttgaagtt cgcaagcagg agctggcgct
     1561 gaatcctcta gtcgcacttg gctggaagga ggtcgttcca ccgggtcagg ttgcagaggc
     1621 acggtaacat gaaagaggca tgctggagat cgtacatcgc ttacagaaga acgtatttct
     1681 agttacactg ggagccgccg ttttcctcgg attctcactg gcatacctgc tgaccaacat
     1741 cattccgttt ttcatttcag acagcggctc cgttcaaatc gccagttcat ctgacaggcg
     1801 tcccgtggaa gtagacgtcg ttcggccttt gcaagatttc gaaggtgtca tcactggaaa
     1861 cttcattcga gattcgggcg atgctggcac caaaggtccc gggacagaaa caggcgaagg
     1921 cgaaatcact ctactgggtg ttctttccgg tccacctcaa ttcgcgcgcg cgatggttca
     1981 gattgtggga aagccagaaa tcgaaacgta tcgcctgggt gaggcagtgg ctggattcaa
     2041 aatcattcaa atccttggca attcagtggt cgtagagcgc ggaagcaaca acctgcggat
     2101 ttacgtaggc gaaaagtcgg gagaagcagc agcggcccct caagctggaa atgcaccgag
     2161 cgcgcagggg gccacaaaga tcaacatcac gcgagatcgt cttcttgcat ccaccaagaa
     2221 cccggaaatg atctatcaga ataaattcgc gccggtgacc cgggacggaa aggtgctcgg
     2281 gctcaagtta ctttttgtcc cgcagggcaa ctttctttac gaactcggtg cacgcagcgg
     2341 tgacattatc cgaagattca atggagagcc cctggagaac caggaccgca tgattcagat
     2401 gtggcagagt ttgcagagtg caaacaggat cacaatcgag gttgagcggg gagggaaagt
     2461 gattcccttc gaaatcctga tccagtgaca cggaagttac aatgaaacag ggatgtaaga
     2521 gccgcaccat tgcacgcata ctgggtctcg cagccctttt tgcggtttcg cacgcgcttg
     2581 cgccccagac tccgcctccc acatctacgc gagattcctg cactcctact aagaatactt
     2641 tctgcgtgag ctatcgcgat atcgaaatcg cggacttctt aaagctcatg gcacagatcg
     2701 ccaaaaagaa cgtactggta gacgaaacca tcaagggcaa gattacgatc ctttcgcaca
     2761 gcccgattcc ggtgaatcgc gcgctggact tcatgaagca ggttctggaa ttgcgcgggt
     2821 tgactgtcct tgaagaacca aacctcgtaa aagtggttcc catggatgtg gcaagttctg
     2881 cgtccattcc ggatccaaac cggcccacgg atcaagacac cactctgact tcgcagatcc
     2941 ttcgcgtacc ttcttctgtg aacataaacg atctggcaaa cgtactgaag agcctcgccg
     3001 ggaaaaatac ggttgtagct ccctacaagg cagcaaacac gatcatcatc acaggctacg
     3061 cgcctaacgt tagacgtgtc ctgtccctgg ccaacgaact gattccacga ctggacgttc
     3121 caggtggaac aaacgtagaa acagataccg ttcacatcta tcgcgtaaag aatatgcctg
     3181 cagaaagtct cgcacagact ctcaccaggc tcgacaatcc tcaggctgct gccacaccgg
     3241 ctcagccagc caatccagcg caaccaggac agcctcctgt tccagttccg gtgcccgttt
     3301 ccgttcctga aactccgggc aaacctgccg ggaagattcg cgcagtgtct cacaaagaat
     3361 cgaattcgat cattgtcact gcatctgacg cggagtggag agagattgaa cagatcattc
     3421 gccagctcga catgttccgt cagcaaattc ttctagaagt tctcatcgca gaggtaagct
     3481 ctacagatct aaacgacttt ggaatcgatt ggagatacca gggcacgaat tccggtcact
     3541 cccagttcaa cactggtctg gctgcggaag gaaagctggt ggatccagac accggaaaga
     3601 tcacaggaaa caacactcta tccggattta gccttggatt cttgaagaaa ggcggagaac
     3661 tccttggcat attcaacgca aatatgaaca accagaactt caacgttcta tccgctccac
     3721 aggttctcac tctcgataac caggaagcgg aaatcaacgt gggccaggac gtcccggttc
     3781 gcacacagga aagaacatct ggcggcggca gctcagaagc aaccgtgaat tcattcgaat
     3841 acagaccgtc cgggatcaag ttgaagttca ctccccatgt gaatgcagaa ggaatgatct
     3901 cgattgattt gttcagcgaa gtgaccaaca ttgaaggcgc aagcagatct gcaggcaatc
     3961 caacgtttaa caagcgtaat gtgaaaacat acatcacagt cggaaacaag caaacgatcg
     4021 taatcggcgg tctcgtagga tcagaaaaac aagaagcagt ttccaaggtt ccacttcttg
     4081 gagacattcc tgtacttgga tttttgtttc gccgcaccac catttcagac aaacgcacca
     4141 acctgatggt cttcattact cctcacattc ttgctgaaaa agcagaagct gatcgattga
     4201 cagatttcaa acgcgaagag caatcacgcg cataccgcca gcgccacaac aacatccgcc
     4261 tgtggcctca aaaggaagtt cctctgggtc gagacgatga cctggacaag atagaggctg
     4321 aatgaaaaag cagctgggag aaatcctgct tcaggaaggg gtcatcacaa aagaagacct
     4381 ccaggaagta ctgaagatcc agaagaaatc tcccctgaaa cttggccaga tcttgcagaa
     4441 aaagggattg gcggatgaag atcaaatcct gaaaactctg gccaatcaat ttggctacga
     4501 gtatgaatcg cgcctgacgt tcaaattcga cgacgcattc aagaagatac ccgtaaacct
     4561 gcttcaaaaa gcgaaaatgg ttcctgtttc tcgcacagga aagagcatca aagttgcact
     4621 gagtgatcca gccgatctac atcctgcaga cgatctaaga aacatcctgg ctgacttccg
     4681 agtgtctttt gtcctggcac ccgaagcaga agtactgaag atcattcatg gtcagttcga
     4741 tcaggcggcc aacgctgcta aagaagtcat ggaaggaatc tcagaagatg aattccagga
     4801 catggacgct ctgggcgatg ataccctgga cctggcaaac gaagctccga tcattcggat
     4861 ggtcaatgcg attttgactc agggcgtgac tgagagagct tcagacattc acatcgagcc
     4921 atttgaaaag agtgtggaag tgcgctaccg cgtggatggg attttgcaca aacgtctctc
     4981 tccgcccaaa gtgattcatg cgggaatcgt gtctcgtatc aagatcatgg caaatttgaa
     5041 cattgctgaa aatcgtcttc cgcaagatgg tcgtattaaa atcaagctct ccggcaaaga
     5101 cgtggacatt cgtgtttcta ccattccgac ccgtcatgga gagagagtgg tgatgcgtct
     5161 tctcaacaag agcgacgtac gtttcactct tgataacatg ggacttccca aaccaatgct
     5221 cgagaccact cagagattgc tgaaggaacc gaacggaatt gtgctggtga cgggtcccac
     5281 gggttccggt aaatctacaa cgttatacgc ctttcttcaa gaaatgaatc aggaatcgag
     5341 aaatatcctg actgcggaag acccggtaga atacgaaatc gaaggcattt cgcagatgca
     5401 aatgcaggag aaaattggcc tgacattcgc aacagctctt cgcgccatgc ttcgtcagga
     5461 tccagacgtc atcatggtgg gtgagattcg cgaccaggaa acagcgcgga tcgccattca
     5521 ggcagcgctc acaggtcacc ttgtattgtc cactcttcac acaaacgatg ctccctccgc
     5581 agtcactcgt cttgtagata tgggaatcga accgtatctc atcacgtcca ccgttcgcgc
     5641 agtgctcgca cagagactgt ctcgcgtgct ctgtccttct tgcaaggaac cgtttcgcgc
     5701 atcggcacgg gatctggcaa gcctgggtct ttctggtgcg gaggcaggaa aaatctacaa
     5761 acatgtgggc tgcgagaatt gcgtgggcac aggataccgc ggaagaaccg gtctttacga
     5821 attgatgctg atggacgaag acgtgcagag aattgtaatt cgcggcggag attcagaagc
     5881 agtgaaaaag gttgcgctcg agaagggcat gcaaacactc cagacttacg gcaagaataa
     5941 ggtactcgat ggagtcacaa ccatcgagga agttctccgc gtgacctgat catgcccatt
     6001 tacaaat
//
LOCUS       DAORFF010000190         6007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7040_length_6007_cov_3.147177, whole genome shotgun sequence.
ACCESSION   DAORFF010000190 DAORFF010000000
VERSION     DAORFF010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7040_length_6007_cov_3.147177"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKK76_09040"
     CDS             <1..473
                     /locus_tag="PKK76_09040"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNL68660.1"
                     /translation="RITSRGDGLSRLRGPCKISIHLMRPGEDPLAPLQPDFIPDLKPA
                     TMDAMRPTKDGASARIVCDLEVDLTGSWVYAARELWAEKDSVYVYEILGARIVDSEGK
                     DRGEVKDYFETGSTGVLTVDSGGKVVMIPSIDQFVDYSRLKEGIIIVPTFDDFL"
     gene            473..1141
                     /gene="trmD"
                     /locus_tag="PKK76_09045"
     CDS             473..1141
                     /gene="trmD"
                     /locus_tag="PKK76_09045"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNL68661.1"
                     /translation="MNFQVLTLFPERYEAYLNSGLPARAQAKGLFKIDPVRLRDYADP
                     GRGGRVDDTPYGGGPGMLLEVGPIDRALKARVEKSPVILFSPRGKRLDQRMVERYAGL
                     PGLTLVCGYYEGVDERVSEHLVNEIVSLGDFVLGSGDLAALCLIEAVTRLLPGYMGSD
                     ESGVEESSGPEGLLEYPQYTRPRDYQGWTVPEVLLSGDHARIREWRRQQQELITRQRE
                     NERS"
     gene            1128..1679
                     /gene="rplS"
                     /locus_tag="PKK76_09050"
     CDS             1128..1679
                     /gene="rplS"
                     /locus_tag="PKK76_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNL68662.1"
                     /translation="MSEVKDETQTETAEAPATPKQVEKIALTVGGGVIYTSLPGLKPK
                     VHPEFNVGDTVRVHYRIKEGDKERVQIYEGTVASIRGEGLARTFLVRRISHDIGVERI
                     FPYESPSIAKIEVMRKGKVRRAKLFYLRHKSGKEGRIKEQFQEIESGKPAAKAPRAKA
                     AKAEKAPKAEKAKPAKAKKKASA"
     gene            1597..2331
                     /locus_tag="PKK76_09055"
     CDS             1597..2331
                     /locus_tag="PKK76_09055"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNL68663.1"
                     /translation="MRKQRKRKRLQKLKRQNQPKQRKKPPRKDFEALEADYSARGFVV
                     AGADEAGRGAYAGPLAVGFVVFSPEVLTRIPSELKEVNDSKQLSAADRLRLATVIRAH
                     AQFCCVVHVSNRKIDRMGINPSTEFALLRALRRAEIAGIPVSAILMDGRFRFERLQKK
                     VKVETLVQGDSRVLSIAAASILAKTSRDARMDRFGSIFPQYSLADHKGYGTKAHRDEI
                     KRNGVTRLHRQSYGIIEQPMLDFGDQ"
     gene            2328..2987
                     /locus_tag="PKK76_09060"
     CDS             2328..2987
                     /locus_tag="PKK76_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68664.1"
                     /translation="MIRVNLVTDIRPGLPQTGQERFRFLGLTPEGLVLLSHAGKLIHA
                     RMDGKPDILPAPGDFFTAKVKQAGDQLELSLLKADSSKSEARAPEIPREILGVFAELL
                     HKDDLPAAEILRLHAYPMSDFLVVPGTLQEKKRRGSLQSGHGKLTGPYYFLLRTRFDK
                     LGEIGILLYADNPDFSGVRALLTAPNEYVRARLAERLRELREIDGRVISNSTHVVDRS
                     A"
     gene            2987..3256
                     /locus_tag="PKK76_09065"
     CDS             2987..3256
                     /locus_tag="PKK76_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNL68665.1"
                     /translation="MEEQKKAAALRFDPATDRAPRIVASGVGTLAEDIVRTAEESGVK
                     VVEDGNLAGFLLDLPAGIEIPENLYKAVSSIFAMLIRLEGEESSL"
     gene            3253..4434
                     /locus_tag="PKK76_09070"
     CDS             3253..4434
                     /locus_tag="PKK76_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNL68666.1"
                     /translation="MKKLQVSELKPGMVFDQPVYIDSSNILLQSGQELLAKDIERLQK
                     WGIHEVMTAGTLIQSGGATGDSLPPPVSAKDPEIQKLESDYEAFRKARFNIKSMVQQG
                     SDILTANMHALVEGKPFDNHALLNLAGKIVDEYHARRFFLLSFQGMTIKAIPQVYHNI
                     HAACYAVAIAQSQNFTRPRTQELVFSILAMDAGMYQMPAHVREKSSALADPERNAVKS
                     HTILGNQLLLKGGKVKPALANIALQHHEHFDGTGYPQNLKAAQIDELARIALIVDSYT
                     AMIEKRNYRPGMLPYDAMRTMLSQDTARYDPKLLRAFLGKVSIYPVGSIVQLSDKRLA
                     LTLGCRPEKPLRPLLRLLRDESGLPYNKATFVDLFVSSELYIVRAVDPSVTGVDLEAE
                     I"
     gene            4458..4763
                     /locus_tag="PKK76_09075"
     CDS             4458..4763
                     /locus_tag="PKK76_09075"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNL68667.1"
                     /translation="MAWEFLRANGHELLAHNFHAKFAEIDLITRDRQGIIHIVEVKAY
                     RGETHPLTAITRERENLMKKAAGVWMATVGEREGISSVTFDLLWVKEEGIETFQNLF"
     gene            4876..5076
                     /locus_tag="PKK76_09080"
     CDS             4876..5076
                     /locus_tag="PKK76_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016757483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68668.1"
                     /translation="MKTLIEAEEPLLEDYIGPDPLLMKKRGLPIQGPRNIKAYKKKIK
                     DEMYMDHAINRIAMELMHFLVR"
     gene            complement(5054..>6007)
                     /locus_tag="PKK76_09085"
     CDS             complement(5054..>6007)
                     /locus_tag="PKK76_09085"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL68669.1"
                     /translation="AARFEIKTQDSEMDYLRLPESVERAVAIASAGWHSILLMGPPGT
                     GKSSCARAIAGLLPPPDETEAIEIRSIHPEMGNTISRPVRIPHHSSTLTALLGGGNPV
                     RAGEVTRAHNGLLILDELAEFSRPVLQSLREPVQEQIVRISRGNDSAILPARFLLAAT
                     SNPCPCGHRDDPRTRCTCGDHQIRSYIQKITGPLLDRLDLQVDVPAAGGAEISSHVTK
                     IRVARAISRQKHRFGKGSLRFNGLLPPDRLKDMISLSQSGRKYLEGVCAPSFRRLHSL
                     LRVSRTIADLDDCEEVHDSHLLEAFHYSCSDYFRINGPGNA"
BASE COUNT         1544 a         1495 c         1631 g         1337 t
ORIGIN      
        1 accggatcac aagtcgcggg gacggccttt ccaggcttcg tggtccgtgc aaaatcagca
       61 ttcacttgat gcgtcctgga gaagatccac tcgcacccct ccagccggat ttcataccgg
      121 atctaaaacc ggccacaatg gacgcgatgc gacccacgaa ggacggcgct tctgctcgga
      181 tcgtctgcga cctcgaagtc gacctgactg gatcctgggt gtatgccgcc cgggaactct
      241 gggctgagaa ggattccgtt tacgtttatg aaattctagg cgcacgcatc gtggattcgg
      301 aagggaaaga cagaggagaa gtcaaagact acttcgaaac aggctccacc ggcgttctca
      361 ctgtagattc cggcggaaag gttgtgatga tacccagcat agatcagttc gtggattact
      421 ccaggctcaa ggaaggaatc atcatcgttc ctacctttga cgattttctc tgatgaattt
      481 ccaggtgctc actctctttc ctgagcgcta cgaggcttac ctgaattcgg gcttgcccgc
      541 cagagcccag gcgaaaggtt tgttcaaaat cgaccctgta cgcttgcggg actatgcaga
      601 tcccggcagg ggaggcagag tggacgacac gccgtatggc ggaggtcccg ggatgctcct
      661 ggaagtgggg ccaattgatc gggctctgaa agccagggtt gaaaagtctc ccgtcattct
      721 gttttctccc agaggcaaga ggctcgacca gagaatggtg gagagatacg cagggctgcc
      781 cggtctaaca ctcgtttgcg gctactatga aggcgtggat gagagagttt ccgagcatct
      841 ggtgaatgaa attgtttcac tgggtgattt cgttcttggt tctggtgatc tggctgcact
      901 ctgtttgatt gaggctgtca ccaggctgct ccctggttac atgggatcag atgaaagtgg
      961 cgtggaggaa agcagtggcc cggaaggtct gctcgaatat ccgcaataca caagaccaag
     1021 agattatcaa ggatggactg ttcccgaggt gttgctctcg ggcgatcatg cgcgaattcg
     1081 cgaatggcgt aggcagcaac aagaactgat tacgaggcag agagaaaatg agcgaagtta
     1141 aagacgaaac acaaacagaa acggcagaag cgccagcaac tccaaagcaa gtggaaaaaa
     1201 ttgcacttac tgtaggcggc ggagtcatct acaccagcct gccaggcctc aaaccaaaag
     1261 tacatcctga attcaatgtg ggtgacactg tgcgcgtgca ctacagaatc aaagaaggcg
     1321 ataaagagcg cgttcagatc tacgaaggaa cggttgcgtc gattcgtggt gaaggtcttg
     1381 ccagaacatt cctggtgcgc aggatttcac acgacattgg tgtagagcgt atcttccctt
     1441 acgagtctcc atccatcgcg aagattgaag ttatgcgcaa aggtaaagtg cgtcgcgcga
     1501 aactattcta cctgagacac aagtccggta aagaagggcg catcaaagag cagttccagg
     1561 aaattgaatc tggaaaaccg gctgcgaaag cgccgcgtgc gaaagcagcg aaagcggaaa
     1621 aggctccaaa agctgaaaag gcaaaaccag ccaaagcaaa gaaaaaagcc tccgcgtaag
     1681 gactttgaag ccctcgaagc cgactactcg gccagagggt ttgtagtcgc aggagccgac
     1741 gaagcaggtc gtggcgctta tgccgggccg cttgctgtcg gctttgttgt tttttccccc
     1801 gaagtgctca ctcgcatccc gtctgagctc aaagaagtaa acgattcaaa acaattgagt
     1861 gccgcagatc gtctaagact ggcgactgtg attcgcgctc acgcgcagtt ctgttgcgtg
     1921 gtccacgtat ccaatcgcaa gatagacaga atgggcatca atccttcgac agaatttgcg
     1981 ctcttgcgcg ctctcagacg ggcagagatt gccggcattc cagtgtccgc cattcttatg
     2041 gacggaagat ttcgtttcga gcgtctgcaa aagaaggtga aggtggagac attggtccag
     2101 ggggattcac gagtcctgtc gattgccgcg gcctccattc ttgcaaagac ttccagggat
     2161 gctcgcatgg atcgatttgg ttccatattt ccgcagtatt cactcgcaga ccacaaggga
     2221 tatggtacaa aagcgcacag ggacgaaatc aagcggaacg gcgtaaccag gcttcacagg
     2281 cagtcatatg gcatcataga acagccgatg cttgatttcg gagaccaatg attcgcgtaa
     2341 atctggttac agacattcgt ccgggactgc cgcaaacagg ccaggaaaga ttcagattcc
     2401 ttggtctaac gcctgaggga ctggtactcc tcagccatgc aggtaaattg attcatgcaa
     2461 ggatggacgg gaagccagac atactgcccg caccaggaga cttctttacc gcaaaggtga
     2521 agcaggcggg agaccagctc gagctctcgc ttctaaaagc ggactccagc aagtcagagg
     2581 cccgcgcgcc cgaaattcct cgggagatac tcggggtttt cgctgaacta ctgcataaag
     2641 acgacctacc cgcagctgaa atcctgaggc tccatgcata ccccatgtcc gatttcctcg
     2701 tggtgccagg cactctgcag gaaaaaaaga gaaggggatc gctccagagc ggccacggga
     2761 agctcacagg gccgtattac tttctgttgc gcaccaggtt tgacaaactc ggcgaaatcg
     2821 ggattctact ctatgcagac aatccagact tcagtggagt aagagcgctg ctgactgccc
     2881 cgaacgaata cgtgcgcgct cgtctcgccg aacgtctcag agagcttcgc gagattgacg
     2941 gccgggtgat ttcaaattcg acgcacgttg tggacaggag cgcataatgg aagagcagaa
     3001 gaaggcggcc gctttacgct tcgacccggc cacagaccgg gctccgcgca tcgtggcgag
     3061 cggagtaggc actcttgcgg aagatatagt tcgaaccgca gaggagagtg gagtaaaagt
     3121 ggtggaggac ggaaaccttg caggatttct acttgatctg cctgcgggaa ttgagatacc
     3181 tgaaaacctg tataaagctg tgtcgtccat attcgcaatg ctcattcggc ttgagggaga
     3241 agagtcctcc ctatgaagaa gctccaggtt tctgaactaa agccaggtat ggtatttgac
     3301 caaccagtct atattgattc atccaacata ctgttgcaga gtggtcagga gttgctggcc
     3361 aaagacattg agagattgca gaagtggggc attcatgaag tcatgactgc cggcaccctc
     3421 attcagtcag gcggggcaac tggtgattct cttcctcctc cggtttcagc aaaggatcct
     3481 gaaattcaga aactggaaag cgattacgaa gcatttcgca aggctagatt caatattaaa
     3541 agcatggtgc agcagggatc ggacatactg actgcgaaca tgcatgctct tgtagaaggt
     3601 aaaccattcg acaatcatgc cctgctgaat ctggcgggaa agatcgtgga cgaatatcac
     3661 gctcgccgtt tctttctttt gtcttttcag ggaatgacaa tcaaagcaat tccgcaggtg
     3721 tatcacaaca tccacgctgc ctgctacgcg gtggccatcg cacagagcca gaacttcact
     3781 cgacctcgca cgcaagaact tgtcttttct attctcgcta tggatgcagg catgtaccaa
     3841 atgcccgcgc atgtcagaga aaaatcaagc gcgcttgcag atccggaaag aaatgcggta
     3901 aagagtcata caattctggg aaatcaactt ttgctaaaag gcgggaaggt gaagcccgca
     3961 cttgcaaaca ttgcattgca acatcatgag cattttgacg gaaccggtta tccacagaat
     4021 ttgaaggcgg cgcagataga tgaactcgca cgcattgccc tcatcgtgga ttcgtacaca
     4081 gccatgatag agaaacgcaa ttatcgtccg ggaatgctcc cttacgacgc gatgcgcacc
     4141 atgctttccc aggacacagc tcgctatgat ccaaaattac tcagagcgtt tctgggcaag
     4201 gtttctatct atccagttgg ttctattgtt caattgagcg ataaaagact ggccctcact
     4261 ctgggttgca ggccggagaa gccacttcgt ccgttgctcc ggcttctacg cgatgaatcg
     4321 ggcctcccat acaacaaagc tacgttcgtc gatctgttcg tttcatctga gctctacatc
     4381 gtgcgcgctg tggatccatc tgtgacaggt gtggatctgg aagcagagat ctgaaattca
     4441 gacatccagc agaagaattg gcctgggaat ttctccgggc aaacggacat gagcttcttg
     4501 ctcacaattt tcacgcaaaa tttgcggaga ttgacctgat cacccgtgac cggcaaggaa
     4561 tcattcatat agttgaagtc aaggcgtaca gaggggagac tcatccactc actgcgataa
     4621 ccagggagcg cgaaaacctc atgaaaaagg ctgccggggt gtggatggcg actgtcgggg
     4681 aacgcgaagg gatatcttct gtcacattcg atctgctctg ggtgaaagaa gagggaatcg
     4741 aaacatttca gaaccttttt tgatccatcc ggattgaaaa atgttcatgc ttcggtcggg
     4801 accgtcgaca gagggtatgt gcggcggagc tgcacgggtt cgcacggatg gataacttgt
     4861 tacagggacg taactatgaa aacactcatc gaggcagaag agcctctgct cgaggattac
     4921 attggtccgg atcctctctt gatgaaaaag agagggcttc ctatccaggg gcctcggaac
     4981 ataaaagcct acaaaaaaaa gatcaaggac gaaatgtaca tggatcatgc gatcaaccgg
     5041 atcgcaatgg agctcatgca tttcctggtc cgttgattct aaaataatct gaacacgaat
     5101 agtgaaacgc ctccagcaaa tgagagtcgt gcacttcctc gcagtcatct agatccgcaa
     5161 tggttctgga gactctgagg agtgagtgca accttctgaa gctgggggcg cacacccctt
     5221 ccagatattt cctgcctgac tgagaaagag aaatcatatc cttcaagcga tccggcggaa
     5281 gcaggccgtt gaatctcaaa cttccttttc cgaatcgatg cttttgacgc gaaatcgccc
     5341 gggctactct gatcttcgta acgtgcgacg aaatttcggc gccacctgcc gcaggaacgt
     5401 caacttgcag atccagcctg tcaagcaaag gccctgtgat cttctgaata tagctgcgaa
     5461 tctgatgatc cccgcatgtg caccgggtgc gaggatcatc cctgtgcccg cagggacatg
     5521 ggttgctggt ggctgcgagt aagaaacgcg ctggaagaat ggcgctgtca tttcctctgg
     5581 aaattcgcac gatctgttcc tggaccggtt ctcttagact ttgcagaact ggtcttgaaa
     5641 attcagccag ctcatccagg atcagaagac cgttatgcgc ccttgtgacc tccccggcgc
     5701 gcacggggtt gccgccgccc agaagagcgg ttaacgtgct tgagtgatgt gggattcgca
     5761 ccggtcttga aattgtgttt cccatttcag ggtgaatgga tctaatttcg attgcctccg
     5821 tctcatccgg aggaggcaat agccctgcaa tggcacgcgc acaagaactc tttccagtgc
     5881 cgggaggtcc catcaacaga atcgaatgcc agcctgcaga ggcgatcgcc acggctcgct
     5941 ctactgattc tggaaggcga aggtagtcca tttcagaatc ttgcgttttg atttcgaatc
     6001 gagcggc
//
LOCUS       DAORFF010000191         5982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7079_length_5982_cov_3.619875, whole genome shotgun sequence.
ACCESSION   DAORFF010000191 DAORFF010000000
VERSION     DAORFF010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7079_length_5982_cov_3.619875"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="PKK76_09090"
     CDS             <1..1278
                     /locus_tag="PKK76_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNL68670.1"
                     /translation="VISLIVSIVFIYASAFFLHHQIRKPGLLAAAILIAGTVGAVRAD
                     RPALHAFVASDVKDYISGSPLGKPASQNLDVRDYQDASARSLPVSGKYKRVILFVMES
                     ITVKELARDCADSSCFPQKYANITHSYKNYYAANQDSRTGILSLLYSAFIPFPAYADR
                     DVEQYEFIGKSKSFLDALHQSGYTTAFSASLSDRERVIMDMPWKKVFRLSEEEAKGGG
                     KFVCLHPYEFERSCEDRILFDRIMDFVSRDPRPFLMHEFVYGHSSQYNEIEKKTSSRY
                     YSEFISDVISGLEARGLLDETLIIVTSDHGIRDRAAMTETESYHIPLLFIQKNFRRTE
                     NSAFVSPIDFRRIFAAEERGEVIPSTRKILPIMGSTGSAMTGILHGVTGLVLIKNRRF
                     GARALETSCHDCPEASDVLPMLEQYRRAFRQIR"
     gene            1449..2354
                     /gene="xerD"
                     /locus_tag="PKK76_09095"
     CDS             1449..2354
                     /gene="xerD"
                     /locus_tag="PKK76_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNL68671.1"
                     /translation="MAKTVPNTQKLIKDFQDYLLVEKGLSENSILSYHYDLKKFHTYA
                     QSRKKDLLKIDTDDITNFLKDQKRKKISSRSLARSVAALRQFYRYLKEEKRVVRNPVD
                     KVQAPEVQRTLPDYLTLGEINQLFAAIKETDAFEMRDKAIFELLYSSGLRISEACTLR
                     MEDVDMENMFITVRGKGGRERLVPFGEHSKMILSRYLDKARHDILRERQSVYIFISKK
                     GDSLNRKSVWRLLKKYLERTDIRKVVTPHTFRHSFATHMIENNADLRSVQELLGHIDI
                     STTQVYTHLANRTLKEVHKKYHPRG"
     gene            2583..2921
                     /locus_tag="PKK76_09100"
     CDS             2583..2921
                     /locus_tag="PKK76_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68672.1"
                     /translation="MKLTALVAIGSALLLSACETYKMGTVSVLPAKTGTEYELVTTAA
                     MKAHNRPIIFGVTFGEEKNFMDVMNDLQASAGCALLKKVEMRQYAVTYLGFGSNTTSV
                     KGECYKVKGK"
     gene            2923..3273
                     /locus_tag="PKK76_09105"
     CDS             2923..3273
                     /locus_tag="PKK76_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68673.1"
                     /translation="MKLLKTISLILVTIYLSGCTSYLGKFAILSTKDVDMSKQYEKMK
                     EPVEGSAATHIIILFPTSLSTIPLFAEATIDAMTKSGADFLENAEIETSAFYIPFIYG
                     KFMATVKGTGYKLK"
     gene            3442..4254
                     /locus_tag="PKK76_09110"
     CDS             3442..4254
                     /locus_tag="PKK76_09110"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNL68674.1"
                     /translation="MKPGTYSTPNSVSTTTGRLLARNRTWFYLEYLHIVFRSRALAKK
                     GLYSREAWAKSSADVLALYERRGGQLTVEGLDQLHKLNGPVVFVANHMSTAETQILPG
                     FIQPVRDVTFVVKDSLMKGPVFGPIMRAVDGIGVTQKDARGDFETVMKEGCERLAKGM
                     SVVIFPEGTRSRIFQPSQFNSLGTKLAKRAGVCMMPVALKTDFWGNGKLLRTFGPLDC
                     RKQAHFKFGAPVMPEGNGKEAQEYALEFIQKHVQMWDRKPGQVVELPGFAAT"
     gene            complement(4335..5234)
                     /locus_tag="PKK76_09115"
     CDS             complement(4335..5234)
                     /locus_tag="PKK76_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68675.1"
                     /translation="MFVEFVRKAYDIWRKSGLVFMLESSRLSLRNLRHWPMVGLLALG
                     FLPGCTPSFPDNSANEPMGESLILGAWISANRWLTFNPSSIGGLRFWIRAENIQQSNG
                     TSVTSWADLSGTGNTVVAGTAPTYYSSTNTINSRPVVRFTGTAVLTNAAPVGLTSSDS
                     GSIFAVLRSNQASADILIIGGCGNTARQFQFSTTTISPTKTCVGQAINPAYSWPIGAM
                     HEVAWIQNLATYVEVRADGRLVASGVPAVSGYGTGALNVSNSTASGMDLAELLWFDSA
                     LTTQNITSVECYLGARYGTTTCL"
     gene            5293..>5982
                     /locus_tag="PKK76_09120"
     CDS             5293..>5982
                     /locus_tag="PKK76_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HNL68676.1"
                     /translation="MEFHVSDFVVIAIYFIVSLGAGLLGSRNKSSLQEFFISGGRAPF
                     WLVGTGMVATTFAADTPLAVAGLVGKNGIAGNWIWWCSAAGGMLTVFFFARLWRRSGI
                     TTDLELIELRYSGKAAAILRGSKAIYFGLIINCIILGWVNLAMLKIFQGMIPGIDARW
                     LVTGSAIITALYVAVSGLWGVAITDAFQFAIAMIGCIVLALIAAFHPQIQAAGGIAHV
                     LPPSVYNFFPTF"
BASE COUNT         1629 a         1535 c         1455 g         1363 t
ORIGIN      
        1 gtaatttctc ttatagtgtc tatcgttttc atctatgcga gcgccttctt tctgcatcat
       61 caaatacgaa agcccggcct acttgcggcg gccatcctga tcgcgggaac cgtgggggcc
      121 gtgcgcgcgg acagaccagc actgcacgcg tttgtagcct ccgatgtgaa agactatatc
      181 tccggcagtc ctctgggcaa accagcctca caaaatttgg acgtaagaga ttaccaggac
      241 gcgagcgctc gctctctgcc agtgtccgga aagtacaaaa gagtgattct ttttgtgatg
      301 gaaagcatca cagtcaaaga gctcgcccgc gattgtgcgg actcatcctg ctttccgcag
      361 aaatacgcaa acattacgca ctcctacaag aactactacg cggccaatca ggacagtcgc
      421 acaggaattc tcagtctcct ctattccgct tttattccct tcccggcgta cgcggatcga
      481 gatgtagagc aatacgaatt cattggcaaa agcaaatcgt tcctggatgc gctccatcaa
      541 tccggataca cgaccgcttt ctccgcctct ctctccgatc gcgaaagggt gatcatggac
      601 atgccctgga agaaagtttt tagactcagt gaagaggaag cgaaaggtgg cggcaaattt
      661 gtatgcctgc atccgtacga atttgaaaga agctgcgaag atagaatctt gttcgatcga
      721 atcatggact ttgtttcgcg tgatcctcgc cctttcctga tgcacgaatt tgtatacggt
      781 cacagcagtc agtacaacga aatagaaaag aaaacctctt cgcgatacta ttctgaattc
      841 atctcagatg tgatctctgg actcgaagcc cgcggtctgt tagacgaaac tctcatcatt
      901 gttacatcag atcacggcat ccgggatcga gcagccatga cagaaactga aagctatcac
      961 attccgttgc ttttcattca aaaaaatttc cgccgcacgg aaaactcagc cttcgtttct
     1021 cccattgact ttcgcagaat tttcgcggca gaagaacgcg gagaagtcat tccatctacg
     1081 cgcaagattc ttcctatcat gggatccact ggcagtgcga tgactggtat tctacacggc
     1141 gtcacaggtc tggtgctgat caagaaccgc agattcggtg cgagagcgct cgaaacatcg
     1201 tgccacgact gcccggaagc ctcggacgta ctacccatgc tcgaacaata ccgccgggcc
     1261 tttcggcaaa tccgctgaaa accggctgaa ttttgttgct aaaatcaaaa gaaaaagtac
     1321 attcgaatta tgtccggccc caggagacag aacgaagaat taaagcgatg tgactaaatg
     1381 cggcataaag ggcaaaaaag ttgccttcgc tttggccagt tctccgaaaa tttatcaggg
     1441 agactagcgt ggccaagact gtaccaaaca cacaaaaact gatcaaagat tttcaggact
     1501 accttctcgt agaaaagggg ctgtctgaaa attctatcct ttcctatcac tacgacctca
     1561 agaaattcca cacctacgcg cagtcccgca aaaaggacct gctcaaaatt gacaccgatg
     1621 acattactaa tttcctcaaa gatcagaaga gaaagaaaat atccagccga tccctggctc
     1681 gttccgtagc agcactccgc cagttctatc gctatttgaa agaagagaag cgcgtcgtgc
     1741 gcaatccagt cgacaaagtg caggcaccgg aagtgcagag aacgcttcct gactacctga
     1801 cactgggcga aatcaaccag ctatttgccg cgatcaaaga aacagacgca tttgaaatga
     1861 gggataaggc gattttcgaa cttctgtatt caagtggtct acgtatctca gaagcgtgca
     1921 cgctgcgcat ggaagacgta gacatggaaa acatgtttat cacagttcga ggcaaaggtg
     1981 ggcgcgaaag actggttccg tttggagagc attccaaaat gattctgtcg cgttacctgg
     2041 acaaggctcg tcatgacatc cttcgcgaac gccagagcgt ttacatattc atcagcaaga
     2101 agggagactc tctcaatcgc aaatcagtct ggagattgct caagaaatat ctggaacgaa
     2161 cggacatcag gaaagttgta actcctcata cattccggca tagctttgcg acacatatga
     2221 ttgaaaacaa tgcggacctc agaagtgtgc aggaactcct gggccacatt gacatttcga
     2281 ccactcaggt gtatacacac ctggccaatc gtactctcaa agaagtccac aagaaatacc
     2341 atccgcgcgg ataacaccgc gcgccaaaat tgagcctgga atgggccgct gtaagcggaa
     2401 atgagtaccc cgccaccaaa cgcctgtcaa ggcgaaaaac taaaggagta ttagcaactc
     2461 aggcgtccga atgaacgcct cttttttggt aatgaacaac acatggctcc agaaaggctc
     2521 gattgctggc atttttgctt gcctcttttt gtcaatcagc acgacctgca ggcgaggact
     2581 aaatgaagct tacagcgtta gtcgcgatcg gatcagcatt gctgctctcc gcttgcgaga
     2641 catataaaat gggaaccgtc agcgtgcttc ccgcaaaaac aggaaccgaa tatgaactgg
     2701 ttactacggc ggccatgaaa gcccacaaca ggccaattat ctttggcgtc acttttggcg
     2761 aagaaaagaa tttcatggat gtgatgaacg acctgcaagc gagtgcgggc tgcgcccttc
     2821 tcaagaaggt tgaaatgaga caatatgcgg tgacgtatct tggattcggg agcaacacca
     2881 cttccgtcaa aggtgaatgc tacaaggtaa aaggtaaata atatgaaact attaaaaacg
     2941 atctcgctta tcctggtaac catctatctc tctggttgca cttcctacct gggcaaattc
     3001 gcaattcttt ccaccaaaga cgtggatatg tccaagcaat acgaaaagat gaaggaaccg
     3061 gtggaaggat cagccgcaac ccacatcatc atcttattcc caacttctct cagcactatc
     3121 ccgctatttg cagaagcgac gatcgatgcg atgacaaaat cgggagcaga tttcctcgaa
     3181 aatgcggaaa ttgagacatc cgctttctac atacctttca tctacggcaa attcatggcg
     3241 actgtgaaag gaacaggcta caaactcaaa taaactcaac gagagccaat agcaaggttg
     3301 aacgttattg gctctctctt cttcccggca tagtctgtaa tgcagactcc aatcgtaaat
     3361 aaaagcgttc tcttttttgc aacctttccg gctaaaccga ttcatacatt aaaactaaca
     3421 acgttaaata caggaagatg tatgaaacca ggcacctatt ctacaccaaa ttcagtttcg
     3481 actacgaccg ggcgacttct ggctcgcaac cgcacctggt tttacctgga atatctacac
     3541 attgtgtttc gttcgcgggc actggcaaag aaaggactct acagcagaga agcgtgggca
     3601 aaaagctcag cagatgttct ggcactgtat gaacgcagag gcggacaact cacggtagaa
     3661 ggcctcgatc aactccacaa actgaatgga ccggtagtat tcgtcgccaa tcacatgagc
     3721 actgcagaaa ctcagatcct tccaggcttt atccagccag tacgcgacgt cacattcgta
     3781 gtgaaagatt ctctcatgaa gggcccggtg ttcggtccaa tcatgcgcgc ggtggacggc
     3841 attggcgtca cacagaaaga tgcgcgaggg gattttgaaa cagtgatgaa ggaaggatgc
     3901 gaacgcctcg cgaagggaat gtcagtcgtg atcttcccgg aagggactcg cagcagaatc
     3961 tttcaaccgt cccagttcaa ttcactgggt acaaagctcg cgaagagagc gggagtgtgc
     4021 atgatgcccg ttgcattgaa aactgatttc tggggcaatg gcaaattgct tcggacattt
     4081 ggcccgctcg attgtcgcaa gcaggcccat ttcaagtttg gtgctcctgt gatgccagaa
     4141 ggaaacggaa aggaagcgca ggagtacgcg ctcgagttta ttcagaaaca cgtgcaaatg
     4201 tgggacagaa aaccgggcca ggttgtagaa ctgcctggat ttgctgccac atagcccgat
     4261 cgccacgcaa aacgactgta gcgcaaaaaa ccaggtcgca gagctgagca acgccgccta
     4321 acggatcaac gcgctcaaag gcatgtggta gttccgtatc gggcgcccag atagcattct
     4381 acgctggtga tattctgcgt tgtgagtgca ctgtcaaacc agagcagctc cgcaagatcc
     4441 atcccggacg cagtcgaatt gctaacattc aaagctccag tgccatagcc ggaaaccgcg
     4501 ggcactcccg aggcgacaag acgcccgtct gcgcgcacct ccacgtaggt agcaagattc
     4561 tgaatccagg cgacttcgtg cattgctccg atcggccagg aataggctgg gttgatcgcc
     4621 tgccccacgc aggtcttcgt aggagagatg gtagttgtgg aaaactgaaa ctgcctcgca
     4681 gtgtttccgc acccacctat gatcaaaata tccgcactcg cctggttcga tctcaagact
     4741 gcgaagatgc taccggaatc ggaactggtc aacccgaccg gtgcagcatt ggtcaaaact
     4801 gcagtgccgg tgaagcgcac tacgggccgg ctattgattg tgttggtgct ggaatagtaa
     4861 gtgggtgctg tgcccgccac cacagtattc cctgtcccgc ttagatccgc ccaggatgta
     4921 acggatgttc catttgattg ctgaatgttt tctgcgcgaa tccagaaccg aagaccgcca
     4981 atagaggaag gattgaacgt aagccagcga ttcgcgctga tccaggcacc cagaatcaaa
     5041 gattcgccca ttggctcatt agcagaattg tcagggaagg agggagtgca gccgggcaga
     5101 aagcccagtg ccagaagacc aaccataggc caatggcgca agttacgaag cgaaaggcga
     5161 gacgattcca gcataaacac aagtcctgat ttcctccata tatcgtatgc ctttcgcacg
     5221 aattcaacaa acaaatcttc gcgcggacca tgttttcttt acagggacag gcccaatgat
     5281 cgcctgcacc agatggaatt tcatgtttca gattttgtag tcatcgcaat ctacttcatt
     5341 gtcagcctcg gtgccggact tcttggaagt cggaacaaat cctctcttca ggaatttttt
     5401 atcagcggag gtagagcgcc cttctggctc gtgggcacag gaatggtcgc cactactttc
     5461 gcggcggaca ctcctcttgc agtggcagga cttgttggaa aaaacggaat cgcgggaaac
     5521 tggatctggt ggtgctctgc ggctggagga atgctcacag ttttcttctt cgcacgctta
     5581 tggcgaagat caggaatcac gacagacctg gaactgatcg aactcagata cagcggaaag
     5641 gcggcggcca tccttcgcgg ttccaaagca atctactttg gcctgatcat caactgcatc
     5701 attctcggat gggtcaacct ggcaatgctc aagatttttc agggcatgat tccaggaata
     5761 gacgcgcgat ggctcgtaac cggcagcgca atcatcacgg cgctctacgt cgccgtttct
     5821 ggtttatggg gagtggccat cacggatgcg tttcaattcg caatcgccat gattgggtgc
     5881 atcgtgctcg cgctgatcgc tgcctttcat ccgcaaattc aggcggcagg tggaatcgca
     5941 catgttcttc cgccctctgt ctacaatttc tttccaacgt tc
//
LOCUS       DAORFF010000192         5955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7134_length_5955_cov_4.250678, whole genome shotgun sequence.
ACCESSION   DAORFF010000192 DAORFF010000000
VERSION     DAORFF010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7134_length_5955_cov_4.250678"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PKK76_09125"
     CDS             <1..284
                     /locus_tag="PKK76_09125"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL68677.1"
                     /translation="MLSSGPMLGVVDPIQARFFRTELQAGDRLYLYTDGLVPTKQDAP
                     DLRMHPLHKAIINSGNTDLESQLKEIVRSLRAGQAGFADDATILGISID"
     gene            359..1498
                     /locus_tag="PKK76_09130"
     CDS             359..1498
                     /locus_tag="PKK76_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68678.1"
                     /translation="MRTVIAITTLIMACARPFPMQEREQNFLSALLLYAGFSSCSTPA
                     FSGIRAVDTPGNMGMGAAIAVSRCVIYITYLDNGTGNIKLARSYDGGNSFVLSVVGPT
                     GTNPSIAVSGSTVHIAYFFTAGADLRYARSTDGGETFTAVTLDSVGSVGDQSSVAVAG
                     SNVYIAYYDSTNIDLKLAKSTDSGSTFSISTVDSAGSVGQQTAIAAEGSEVYITHYES
                     AAFDLKLARSIDSGANFINSVIDSAGSVGQYSAVLKDSGNVYVSYYDSGGGDLKLGKS
                     TNAGASFNVFSAETANNVGNYSTAAVLGNTIYVAHYDATNFDLRLLRSYDQGMNFTAS
                     TIETTGDVGQYASVAAESNNLYVAYYDNTNQDLRFARSSNLGVSF"
     gene            complement(1626..2888)
                     /locus_tag="PKK76_09135"
     CDS             complement(1626..2888)
                     /locus_tag="PKK76_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNL68679.1"
                     /translation="MKTKNTTPRSRRAVFIAATIVLVLVAIRAMDALSGWFLDTSTQY
                     YVLPAASTTLGTNSLGLRNREIGPKDGTRILFLGDSFTQGLGVREEDGFVRRSEKLLQ
                     QRFRVEHVNAGMTGFAPGNELGLYRAIRDKVQPDIVVVCLYQNDIYESGESLLLRRMK
                     QNQQKKLVIRALYGIAPVTTNFVYRTLLQKRYHDLLMSQALTLDRTQALLEESDSAQQ
                     RQPASPPLTLNPAMFVIQNEVRKYAREMGIRDEATSEWLARNGPSLVDGRNPQEVILG
                     LLEPEYFSQVYTLDARARPKFETMLKAIQKIKEESEQNKSAFAVVYIPSDDRYDREKF
                     NLMREYGYSMNEEWLDGRVLSDIEIERWMSNHQTPYLSMLSPFRKSREPLTFPRDQHL
                     NKRGHEVMASAVAQFLEGNFASRLQKRN"
     gene            complement(2911..3063)
                     /locus_tag="PKK76_09140"
     CDS             complement(2911..3063)
                     /locus_tag="PKK76_09140"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNL68680.1"
                     /translation="MTLLMEFVAFLRERKKFWILPIFFFVVLLVGLGIVATGAGGLSP
                     FIYALF"
     gene            complement(3073..3441)
                     /locus_tag="PKK76_09145"
     CDS             complement(3073..3441)
                     /locus_tag="PKK76_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68681.1"
                     /translation="MRFFLMIATMAGGIGAYRYWSTGEVSHTFGSISIACFTIAVIPP
                     LANKLFLIWMKLAHGLQFIVLRTLLSIAYILAVVPIGLIARVAGKKFLETIPDRDAET
                     YWENRAQGLNIDPQKYEHHF"
     gene            complement(3480..5312)
                     /locus_tag="PKK76_09150"
     CDS             complement(3480..5312)
                     /locus_tag="PKK76_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HNL68682.1"
                     /translation="MTRILGISCYYHDAAATLVQDGDIIAAAQEERFTRKKHDPRFPE
                     SAIEYVLSEGNISQAEIDIVAFYDKPFIKLERILESILQASPRGYRLWLAAMPSWIDH
                     KLLVGRSIRRGLPGLRKDAQILFAEHHESHAAAAFYPSPFEKAAFLTLDGVGEWTTTS
                     WGEGSGNQLQIRQEIKFPHSLGMLYSAFTYYAGFKVNSGEYKLMGLAPYGEPSYAQKI
                     RDHLIDIKPDGSFRLNMDYFDYSAGLRMTNSSFDRLFGGPPRKPESPLTRREMDLARS
                     VQVVTEECILKLGRHVHASTGMDYLCLSGGVALNCVANGRLLREGPFKEIWIQPAAGD
                     AGSALGAALVVWHRVLGKDRDPFRAGKIQKGSYLGPRFKGTDIRTFLDSVNAKYLRVP
                     DTFGAARIAAKYIADGKVVGWFVGRMEFGPRALGARSILGDPRNERMQSIMNLKIKFR
                     ESFRPFAPSVLREDVAEYFEHEKDSDYMLIVAPVKQSIRRQMTQEQESLFGIDKLNVM
                     RSNLPAVTHVDYSARVQTVSPENHPEYYELISEFKRLTGCGVIINTSFNVRGEPIVMS
                     PEQAYTCFLRTGMDVLVLENFVLLKEEQDKLPEDAHWQKEFELD"
     gene            complement(5457..>5955)
                     /locus_tag="PKK76_09155"
     CDS             complement(5457..>5955)
                     /locus_tag="PKK76_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNL68683.1"
                     /translation="PAYVFEFGYLPYSTAVVYFWIFLIPLDFLILADRIRMLRTEKSR
                     YAKSRITGLDVSSIVTKMEKILTIDQLLRDPTLRLPDLARALDIPAYQLSEILNNVVQ
                     KSFREYINGLRIQTAQRSFTVDPDADIDTVMRENGFRSRSVFNHAFKSQTGQTPVEFK
                     RSSQK"
BASE COUNT         1425 a         1469 c         1530 g         1531 t
ORIGIN      
        1 aaatgctcag cagcggacca atgctcgggg tagtggatcc aattcaggcg cgtttctttc
       61 gcacggagct gcaggcaggg gatcgccttt atctctatac ggacggtctt gttccgacaa
      121 agcaggatgc cccagatctt agaatgcatc cccttcataa agcaatcatc aatagtggga
      181 atacggacct tgagtcgcaa ttgaaagaga ttgtgcgttc cctgcgcgca ggtcaggccg
      241 gttttgcaga tgatgcgact attttaggaa tatctatcga ttagtcctgc cctgaaagtc
      301 ctggtgcagt gatgcgaaag aacttgactg aactgcttcc gcgttccaga tttcgatcat
      361 gcgcacggtg attgcaatca caacgctgat tatggcttgc gctcgccctt tcccaatgca
      421 agagcgagag caaaatttcc tcagcgctct gcttctgtac gcgggcttct cttcctgttc
      481 cacaccggcg ttttcaggaa tacgcgcagt ggacacgccg ggcaatatgg gcatgggcgc
      541 tgcgattgcg gtttcgcggt gcgtaatcta catcacttat ctggacaacg gcaccggaaa
      601 tatcaaactc gcgagatcct atgatggagg aaattcgttt gttctctccg tggtcggacc
      661 tacgggaacc aatccatcta tcgccgtgag cggcagcacg gttcatatag cctatttctt
      721 tacggcggga gcagatcttc gctatgcgag gtctacagat ggaggggaga cattcacggc
      781 ggtcactctt gattcggttg gaagcgtagg agaccaatca tctgttgccg ttgcgggcag
      841 caatgtttac atcgcgtact atgacagcac caacatcgac ctcaagctcg cgaagtccac
      901 ggactccgga tctacgttct ccatatcaac tgttgattca gcggggagcg tgggccagca
      961 aacggcgatt gcggcagagg gtagcgaagt ctacatcact cactacgaat cagcggcatt
     1021 tgacttgaag cttgcgcgtt ccatcgattc cggcgcgaat tttatcaatt ccgtaatcga
     1081 ttctgcgggg agtgtggggc agtattctgc agtgctcaag gatagtggaa acgtgtacgt
     1141 gagctactat gattcgggag gaggcgatct gaagcttggg aaatccacaa atgccggcgc
     1201 atctttcaat gttttttcgg cagaaacggc gaataacgtt gggaattact ctacagcggc
     1261 tgtgctcggg aatacaattt atgttgcgca ctatgacgca accaacttcg atttaaggct
     1321 tctgcgttct tacgatcagg gaatgaactt cacggcttcc acaatcgaaa caacaggcga
     1381 tgtgggacaa tacgcgtctg tggctgcgga atccaataat ctgtatgtgg cttactacga
     1441 taacacgaac caggacctgc gattcgcgcg ttcttccaat ctgggtgtgt ctttctagat
     1501 caggcggggg tcgcgggtct agggttgcat agaatctctc cgcttgaatc cgttccgccg
     1561 tggggccacg cctccaacac aagttgtgag gcaatcttgc accggctcta cgtggaacaa
     1621 tagagttagt ttcgcttttg cagccgcgat gcaaaattcc cttccagaaa ttgagccacc
     1681 gctgaagcca taacctcatg gcctcgtttg ttcaaatgct gatcccgcgg aaaggtgagt
     1741 ggttcgcgcg atttacgaaa tggactgagc atactgagat acggggtctg atgatttgac
     1801 atccaccgct cgatctcaat gtcggagagt acccggccat ccagccattc ttcattcatc
     1861 gaatacccat attcacgcat caggttgaat ttttccctgt catagcgatc atccgacggt
     1921 atgtaaacga cagcaaaggc cgatttgttc tgctccgact cctctttgat tttctgaatc
     1981 gcttttagca ttgtttcaaa tttgggacgc gcgcgcgcgt ccagggtata gacctgtgaa
     2041 aaatactccg gttcaagtaa accaaggatc acttcttgtg gattgcgtcc gtcgacaagt
     2101 gaagggccat ttcgtgctag ccattcgctt gttgcttcat cccggatccc catctccctg
     2161 gcgtattttc tgacctcgtt ttgaatcacg aacatcgcgg gattcagcgt gagtggaggg
     2221 gaagccggtt gtctttgttg agcagaatcg ctttcctcaa gcagtgcttg agtgcgatcg
     2281 agtgtgagtg cctgactcat gagaaggtcg tggtatcttt tctgcagaag cgttcgatat
     2341 acgaaattag tggtaacagg agcgatcccg tacaatgcac gaatgaccag tttcttttgc
     2401 tgattttgct tcattcttcg cagaagtaga ctttctccgg attcgtagat atcattctga
     2461 taaaggcaaa caactacaat gtcgggctgc actttgtctc gaatggcacg atacagtccc
     2521 agttcatttc ccggcgcaaa gcccgtcatt cctgcgttta cgtgttccac gcggaatcgc
     2581 tgctgcagaa gcttttcaga tctgcgaacg aatccgtcct cttcgcgtac gcccagcccc
     2641 tgcgtaaaag aatcacccag aaacagaatt cgcgtcccgt cttttggtcc gatttctcgg
     2701 tttcgcaagc cgagcgaatt cgtaccgagt gttgtgctgg cagcggggag tacataatac
     2761 tgcgttgacg tatcgaggaa ccatccggaa agagcatcca ttgcccggat agccacaaga
     2821 accagaacga tcgtcgccgc gatgaaaaca gcccgacgtg agcgtggcgt tgtattttta
     2881 gtcttcattg tattttcctt tcgatcgtcg ctagaataac gcgtatatga acggtgagag
     2941 gcctcccgcg cccgtggcta ctatgcccag accgaccaat agaaccacga agaagaagat
     3001 cggtagaatc caaaatttct ttctctctct caagaaggcg acaaactcca tcagtagtgt
     3061 catgatcttc tcctaaaagt ggtgctcgta cttttgcggg tcgatattca atccctgtgc
     3121 gcgattctcc cagtaagttt ccgcatctcg gtccgggata gtttccagaa atttctttcc
     3181 cgccaccctg gcaatcaggc caatgggaac tactgccaga atgtaggcga tggacagtag
     3241 cgttcttaga acgataaact gcaggccgtg cgcaagcttc atccagatca aaaacagctt
     3301 gttggccaga ggtgggatga cagcgatcgt gaagcaggcg atggagatag aaccaaacgt
     3361 gtggctcact tcaccggtgc tccagtaacg ataggcgcca atgcctccgg ccatagtcgc
     3421 aatcataagg aagaatcgca ttccgggtga tagtctgcgg tagacatgtc gtaaagcgtt
     3481 caatcgagtt caaactcctt ttgccaatgt gcatcctcgg ggagtttgtc ttgttcttct
     3541 ttcagaagca cgaaattctc gaggacaaga acatccatgc ccgttcgtag gaagcatgta
     3601 taggcctgct ctggtgacat gacgatcggt tcgccccgca cgttgaaaga cgtattgatg
     3661 atgaccccgc atccggtcag ccgtttgaat tcagagatta gctcatagta ctccggatga
     3721 ttctccgggg aaacagtctg tacgcgagca gagtagtcca cgtgcgttac ggcgggaaga
     3781 ttagatcgca taacgttcaa cttgtcaatc ccgaacaaac tctcctgttc ctgagtcatt
     3841 tgcctgcgta tggattgttt gacaggcgcc acgatcagca tgtagtcgga atccttctcg
     3901 tgttcaaaat attctgcaac gtcttcgcgc agcacgcttg gcgcaaaagg tcggaaagat
     3961 tcccggaatt tgatttttag gttcatgatc gattgcattc tctcgtttcg cggatctccg
     4021 agtatactcc gggcccccag ggcgcggggg ccgaactcca tgcggccaac aaaccacccg
     4081 acaacttttc cgtccgcgat gtatttggcg gcgatgcgag ccgctccaaa ggtgtcagga
     4141 actctcaagt atttcgcatt cacggaatcg agaaacgtgc gaatatctgt tcctttgaag
     4201 cggggaccaa ggtaggatcc tttttgtatt ttgcctgcgc gaaatgggtc gcgatccttg
     4261 ccgagcactc gatgccagac caccagggcg gcgcccagag ctgaccctgc atccccggct
     4321 gccggttgaa tccagatttc tttgaatggg ccttcgcgta aaagccttcc gtttgcaaca
     4381 caattgaggg cgactccgcc ggacaaacaa aggtagtcca tccctgttga agcgtgaacg
     4441 tggcgaccga gtttcaaaat acattcttct gtgaccactt gaacggagcg tgcaagatcc
     4501 atctcgcgtc gggtcagcgg ggattcgggt tttcggggag gaccaccgaa gagacggtca
     4561 aagctggaat tggtcattct gagccctgcc gaataatcga aataatccat attcaatcgg
     4621 aaggaaccgt ctggctttat gtcgatgaga tgatctcgta tcttttgagc ataagaaggt
     4681 tccccgtagg gtgccagtcc catcaatttg tattctccgg aatttacctt gaatcccgca
     4741 tagtacgtga acgcggaata cagcatgccg agggaatgcg gaaatttgat ttcttgccgg
     4801 atttgtagct ggtttccgga cccttctccc caggacgttg tggtccattc tccgacgccg
     4861 tcgagggtca ggaatgccgc cttctcaaaa ggagagggat aaaaagcggc tgccgcgtgt
     4921 gattcgtgat gctcggcaaa tagaatctgc gcgtcctttc gcagacccgg taagccacgt
     4981 cgaatgctgc gtcctacgag taatttatga tcgatccagg atggcatcgc cgccagccac
     5041 aatctatatc cgcgggggga tgcctgcaga atcgattcaa ggattctctc taacttgata
     5101 aagggcttat catagaaagc aacgatgtcg atttcagcct gcgagatgtt tccctcagac
     5161 agtacgtatt caattgcgct ttccgggaat cgcgggtcat gcttcttgcg cgtgaagcgc
     5221 tcctcttgtg ctgccgcaat gatatcgcca tcttgcacca gcgttgcggc cgcgtcgtga
     5281 tagtaacaag aaattccgag tattctcgtc atcggatgaa tgagaatcct gactttattt
     5341 tgaaatccag tctttttctt ttcaacgacc aggacggaga caaaaaaaag atcatgcgtg
     5401 cgccgtgcga agatgcgtga aactcttacg agtttataat ttagacatct tctgagttac
     5461 ttctgtgaag agcgtttgaa ctcgaccggt gtttgtccgg tttgagattt gaaagcatgg
     5521 ttaaagacag aacgtgatcg aaatccattt tcgcgcatca ccgtgtctat gtctgcatcg
     5581 ggatccacag tgaatgatcg ctgagcagtc tgtatgcgta gtccatttat atattctctg
     5641 aaggactttt gaaccacatt gttcaaaatt tcagagagct gataagcggg gatatccagc
     5701 gctcttgcga ggtcaggaag gcggagtgta ggatctcgta atagttggtc tattgtaaga
     5761 attttctcca ttttcgtcac gatggaagaa acatccagac cagtgattct tgatttcgca
     5821 taacggcttt tttctgtgcg aagcatgcga attcgatcgg caagaattag gaaatcgagc
     5881 ggaatgagga agatccagaa gtagacaacc gcagtggagt aaggaagata tccaaattcg
     5941 aaaacatatg ctgga
//
LOCUS       DAORFF010000193         5949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7144_length_5949_cov_3.668137, whole genome shotgun sequence.
ACCESSION   DAORFF010000193 DAORFF010000000
VERSION     DAORFF010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7144_length_5949_cov_3.668137"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PKK76_09160"
     CDS             <1..191
                     /locus_tag="PKK76_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNL68684.1"
                     /translation="LPAREQKVLRMRFGLDDGYSHTLEEVGYVFQVTRERIRQIEAKA
                     LRRLRHPSRSKKLKDYID"
     gene            206..1219
                     /locus_tag="PKK76_09165"
     CDS             206..1219
                     /locus_tag="PKK76_09165"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68685.1"
                     /translation="MDRRVALTLATGFTSAVVVCFALFAEAPQGCVKGDCINGPGERV
                     FPDGNRLIVSFKKGMPEGQGKIQYADGGTYEGTFVAGKQQGYGTRNWPGQALYRGQWL
                     QGRIHGQGSIRFASGTTQEGFFQNGMLEGKGKSTLSNGGTYAGDFSAGRFSGKGTLKT
                     PRVTYEGTFKSGRADGKGKAVWTDGSSAEGDFRSGKPHGSCNVRYANGNHFEGEWKDG
                     RPDGQGKYSWADGAVYTGGFSAGMPEGEGEFVAEDRNYKGSFKSGLYSGKGVLGTKAG
                     NRYEGDFEKGSPHGRGVYTFKSGTVYEGDFRDGKFNGKGTLKFKDGTTQEGTFKDGQF
                     TGK"
     gene            complement(1220..3166)
                     /locus_tag="PKK76_09170"
     CDS             complement(1220..3166)
                     /locus_tag="PKK76_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL68686.1"
                     /translation="MKRSRTGNLLPGVILAVGFGFSIGFFLISRDAVDRQVRDSFHLT
                     VSFEAAAIKDNMLMHEKELRELGAAVQGLGGGPALQRYLQGVDFGTNFPGFETIDFIS
                     TSGGLQRLYPAGERLSQNTYAHLQGELGKARAQKFLVAMASPGDDQIITWVQPLDGGD
                     LIVSKVSLARLIHALFRDRAVYTLDPGSQGVYFEVITISTGGLQKHLYFPMPADFEDT
                     AGRTLPYMILGSGSIISLLLFIVIRSLSGARDQALTLAEKMTEDFRRATSEAIRANQA
                     KSEFLASMSHEIRNPIHVITGMSEILAGTNLAEDQRDYLRSLRNAGDHLLGLINDILD
                     LSRIESGQLELEEIDFSLKEAAGQVMELYRYRAQEKGIALNLNFGPDAPDIVRGDPVR
                     FRQILVNLTGNAMKFTAKGSVTIRVEKHPLATEKQPGVHVSVTDTGIGIPADKQAAVF
                     EKFTQVDTSTTRRFGGTGLGLAICKKLTAMMHGDIGLESEPGRGSTFFFRVLFAAPAA
                     PAGTTAAPVAAPIPVSDRPIRILLAEDAEDNRSIIALFLRAVPHELTIAENGEQAVAA
                     FAPGKFDVILMDNQMPVMDGLTAVRQIRTLEREAGKPPTRILALTADSTRKDIERSLD
                     AGCDGHLTKPIRRDVLVQAVRSAT"
     gene            complement(3163..3843)
                     /locus_tag="PKK76_09175"
     CDS             complement(3163..3843)
                     /locus_tag="PKK76_09175"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNL68687.1"
                     /translation="MKRRLLLLVLLPVLCGQTPSRLSRITFARGEIFVTHAGETKPAE
                     SGQILYHDDMIRTGKDSYAEVMVQNVGVFRLAENSELKLEELQSKDKTIIRLDKGKAG
                     FFLLKIPSKEQVQVRLPTAVAGVRGTNFLVSAEKGSKVALFGGAVQMEDTAKKSVVID
                     KPGEITLKAGESLANKKVEKLSAESIADMKKLEEMLPLNLQSVPQDPGRMNQTDLDAP
                     QGSDLKKP"
     gene            3959..5359
                     /gene="leuC"
                     /locus_tag="PKK76_09180"
     CDS             3959..5359
                     /gene="leuC"
                     /locus_tag="PKK76_09180"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNL68688.1"
                     /translation="MKPRTLYDKIRENHIVNEDAESVLLYIDRHLVHEVTSPQAFEGI
                     RMAGRKIRRPDLTFATMDHNVSTRSRDWGAAGELSRIQMETLSKNCKDAGIRIFDLNN
                     PDQGIVHIIGPEMGLTLPGLTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLVH
                     QKSKNMLISIEGDTGPGVTPKDIILYIIGVIGTNGGQGSVIEYSGEAIRKLSMEGRMT
                     ICNMSIEAGARAGLIAPDETTFEYLKGKDFAPRGADFDKAVAYWKTLPSDEGAHYDRV
                     VKIKGSDITPMVTWGTNPGQVASVAAAVPDPDSMSDNVERETARHALKYMGLKAGQKM
                     TDITVDNVFIGSCTNGRIEDLRIAAKLAKGKKVKSGVKAIVVPGSGRVRRQAEEEGLH
                     KVFLDAGFEWRLAGCSMCLGMNDDVLAPEARCASTSNRNFEGRQGRGARTHLVSPQMA
                     VAAAVEGKFVDVRNWN"
     gene            5359..>5949
                     /gene="leuD"
                     /locus_tag="PKK76_09185"
     CDS             5359..>5949
                     /gene="leuD"
                     /locus_tag="PKK76_09185"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNL68689.1"
                     /translation="MQAFTKLSGVAAPLDRSNVDTDQIIPKQFLKKIERSGFGIHLFH
                     DWRFSDADGKVPIADFLLNRAPYNKASILLARENFGCGSSREHAPWALEDFGIRSIIA
                     PSFADIFYNNCFKNGMLPVILPTGQVDELFEYEAKNPGQSFEIDLPAQLIRRPDGKTY
                     SFEVDAFRKECLLNGWDDIGLSLRHADKISSFEKSYF"
BASE COUNT         1479 a         1458 c         1571 g         1441 t
ORIGIN      
        1 ctcttcctgc ccgcgaacag aaggttttgc gcatgagatt tggtctggac gatggatatt
       61 cgcacactct ggaagaagta ggttacgtct tccaggtgac tcgagaaagg attcgccaga
      121 tcgaagcgaa agctctgcgg cgtctgagac atccgtctcg cagcaagaaa ctgaaagact
      181 acatcgactg agaggattcg agcggatgga tcggcgcgta gctcttactc tggcgactgg
      241 gttcacctct gcagtcgtag tatgttttgc actgtttgca gaagcacctc aaggctgcgt
      301 caaaggcgat tgcatcaacg gaccaggtga gcgcgttttc ccggacggaa atcgcctcat
      361 tgtaagtttc aaaaagggca tgccggaagg ccagggtaaa atccagtacg cagacggcgg
      421 cacatacgaa ggcacattcg ttgccggcaa acaacaggga tacggcactc gaaactggcc
      481 aggtcaggct ctttaccgcg gccaatggct gcaggggaga attcacggtc agggttcgat
      541 tcgatttgca agcggcacga ctcaggaagg attttttcaa aacggaatgc tcgaaggaaa
      601 aggcaagtcc actctctcaa acggcggcac atacgcagga gatttttctg ccggcagatt
      661 ttccggaaaa ggaacactca aaacccccag agtgacctac gaaggcactt tcaaatcggg
      721 acgcgcggat ggaaaaggaa aggctgtctg gacagacgga agttcagcgg aaggggattt
      781 caggtcaggc aagccccatg gatcgtgtaa cgtgcgttat gcgaacggaa atcactttga
      841 aggggaatgg aaggacggaa gacccgacgg ccagggaaaa tattcctggg cggatggagc
      901 ggtttacacc ggtggtttct ctgcgggcat gcccgaaggg gaaggcgagt tcgttgcaga
      961 agatcgcaat tataaaggat cgttcaagag cggcctgtat tccgggaagg gagtgctcgg
     1021 aacaaaagcg ggcaatcgtt atgagggaga ttttgaaaaa ggaagtcctc acggccgcgg
     1081 cgtctacacc ttcaaatcgg gcacggtata cgaaggcgat ttcagagacg gtaagtttaa
     1141 cggaaaggga actctgaaat tcaaagacgg aaccactcag gaaggaacgt tcaaagacgg
     1201 tcagttcacg ggcaagtaat caggtggcag atcgcactgc ctggaccagc acgtctctgc
     1261 gaattggttt ggtgagatga ccatcacaac ctgcatccag tgatctttca atgtctttgc
     1321 gcgttgagtc tgcagtaagt gcaagtatcc gagtgggagg ctttcctgct tctctttcca
     1381 gggtccgaat ttgacgaacc gcagtgagtc cgtccatcac tggcatttga ttgtccatca
     1441 ggatcacgtc aaatttacct ggcgcgaatg cagccacagc ctgttctccg ttttccgcaa
     1501 ttgttagctc gtgcgggaca gctcgcagaa aaagagcgat gatgcttcta ttgtcctccg
     1561 catcttctgc aagcaggatg cggatgggtc tgtctgaaac aggaatgggt gccgccacgg
     1621 gtgctgctgt ggtgcctgcg ggagctgcag gagctgcgaa gagtacgcga aagaaaaacg
     1681 tactgccgcg gccgggttca ctttccagac caatgtctcc gtgcatcatc gccgtcagtt
     1741 tcttacagat ggccagacca agtcctgttc cgccaaatct tcttgtggtg ctcgtatcta
     1801 cctgcgtgaa tttttcgaac acggctgcct gcttgtctgc tggaattcct atccctgtgt
     1861 ctgttacgga cacatgaact ccaggctgct tctctgttgc cagcggatgc ttttctactc
     1921 gaatggtgac acttcctttc gcagtgaatt tcatcgcgtt tccagtcaaa ttcaccagaa
     1981 tctggcggaa tcgtactgga tctccgcgca caatgtcagg cgcgtccgga ccaaaattca
     2041 gattcagcgc aattcctttt tcctgagcgc ggtagcgata gagctccatc acctggcccg
     2101 ctgcttcttt cagagaaaag tcgatctctt ctagctccag ttgccctgat tcaatgcgcg
     2161 aaagatccag aatgtcattg atgaggccaa gaagatgatc gcccgcattt cgcagggaac
     2221 gcaaataatc tctctggtct tctgcgagat ttgtgccggc aagaatttcg ctcatgccag
     2281 tgattacgtg gattggattt ctaatttcat gactcatact tgccaggaat tctgatttag
     2341 cctggttggc gcggattgct tcactggtgg cgcgccggaa atcttccgtc attttttctg
     2401 ccagagtcag agcctgatct ctagcaccgg agagtgagcg aatcacaatg aaaagaagaa
     2461 gagagataat cgagccggaa ccaaggatca tgtagggaag agtgcggcct gcagtgtctt
     2521 caaaatctgc tggcatgggg aaatacagat gcttctgcaa tcctcctgtg ctgatggtga
     2581 tcacttcgaa gtaaacaccc tgactgcctg gatccagggt atacactgcg cgatcgcgaa
     2641 agagtgcatg gatcaatctg gccaaactta ctttagaaac tataagatct cctccgtcca
     2701 gaggctgcac ccacgtaatg atctgatcat ctcccgggct tgccattgcc accaggaatt
     2761 tctgagcgcg tgctttcccc aattcgcctt gcagatgcgc gtacgtattt tgagagagtc
     2821 tttcgcctgc agggtagagt ctttgtaatc cgccggaagt ggaaatgaaa tcaatggtct
     2881 cgaatcccgg aaagttggtg ccgaagtcca ctccctgaag atatctctgc aaggctggtc
     2941 ctccgcccag gccctgaact gcagcaccca attcacgcag ctccttttca tgcatcagca
     3001 tgttgtcttt gatcgctgcc gcctcaaagc ttacagtcag gtgaaaggaa tcgcgcacct
     3061 gtctgtccac tgcatcgcgc gaaatcaaga agaagccaat ggagaatccg aaaccaactg
     3121 ccagaatgac tcctggcagc aagttcccgg tgcgagaacg tttcacggtt tctttagatc
     3181 agatccctgg ggggcgtcca ggtctgtctg gttcatcctc cctggatcct gtggaacgga
     3241 ttgcaaattg agaggaagca tctcttccag ttttttcata tccgcgatgg attctgcact
     3301 gagcttttcc actttcttgt tcgcaagaga ttctcctgct ttcaaagtga tctctccggg
     3361 cttgtcgatc acgacacttt ttttggcggt gtcttccatt tgaaccgctc ctccgaagag
     3421 tgccaccttg gagccttttt cagcagacac gaggaagttg gttccgcgaa cacctgccac
     3481 cgccgttggc aatcttacct gcacttgttc tttcgatgga atctttaaca ggaagaagcc
     3541 ggccttacct ttatccaggc gaatgatggt tttgtctttg gattgaagtt cttccagttt
     3601 tagttcggag ttttcagcca gcctgaatac acctacgttt tgaaccatca cctctgcgta
     3661 agaatctttt ccagtgcgaa tcatgtcgtc atggtataag atctggccag actctgctgg
     3721 ttttgtttcc cctgcatgag tgacgaatat ttctccgcgt gcaaaagtaa tgcgggacaa
     3781 tctggatgga gtctgtccgc agagaacggg caaaagtacc agtagaagta ggcgacgttt
     3841 cataagcaaa tggcctcaag cgggcgggtg ccgtccattg aattccaggc atgcccatgt
     3901 aaaaaaagac ataaaatcct ttctggaata gggccttttt cgttttctgt acccggcgat
     3961 gaaaccacgc acattatacg acaaaattcg tgaaaaccac attgtaaatg aggacgcaga
     4021 gtctgtgctt ctctatatag accggcatct ggttcacgaa gtaacttctc ctcaggcttt
     4081 cgaagggatt cgaatggccg gccgtaagat tcgcagaccg gatctaacgt tcgcaaccat
     4141 ggatcacaac gtctccactc gttctagaga ctggggcgct gcaggcgaac tctccagaat
     4201 acagatggag actctgtcta aaaactgcaa agacgccggg attcggattt ttgatttgaa
     4261 caatccagat caagggatcg tccacatcat tggtcctgag atgggtttga ctttgcctgg
     4321 cctcaccatc gtttgcggag attctcatac aagcacgcac ggtgcatttg gtgctcttgc
     4381 atttggaatc ggcacaagcg aagtagagca cgtactggca acgcagactc tggttcatca
     4441 gaagtcaaag aacatgctca tctccattga aggagacact ggacctggtg tgactccgaa
     4501 agacatcatc ctctacatca ttggtgtgat tggcacaaac ggcggccagg gttccgtcat
     4561 cgaatattca ggagaagcca tccgcaaact ttctatggaa gggcgaatga ctatctgcaa
     4621 catgagcatt gaagcaggcg cccgcgcggg tctcatcgct ccggatgaaa ctacatttga
     4681 atatctcaaa ggaaaagatt tcgcaccaag gggcgccgat tttgacaaag cggtggccta
     4741 ctggaagact cttccatcag acgaaggggc acattacgat cgagtggtga agatcaaagg
     4801 ttctgacatc acacctatgg taacctgggg aaccaatcca ggtcaggtcg cgtccgttgc
     4861 tgcggctgtg ccggatccag actcaatgtc ggataacgtg gagcgagaaa cagcacgtca
     4921 tgcgctcaag tacatgggac tgaaagctgg tcagaagatg accgatatca ccgtggacaa
     4981 tgtattcatt ggatcgtgca cgaacggccg cattgaggat cttcgcattg cagcaaaact
     5041 cgcgaaggga aagaaagtca aaagcggagt caaagcaatc gtggttcctg gatctggccg
     5101 ggtgcgcaga caggcagaag aggaaggact tcacaaggta tttctcgatg ccggttttga
     5161 atggagactg gcaggctgtt ctatgtgcct gggcatgaac gatgatgtgc tggcaccgga
     5221 agcaagatgc gcttctactt ccaacagaaa ctttgaaggc agacagggta gaggagcacg
     5281 cactcatctg gtgagtcctc agatggccgt ggccgccgcc gtagaaggca aattcgtaga
     5341 tgtgaggaac tggaactgat gcaggctttt actaaactat caggcgtagc cgccccgctc
     5401 gaccggtcca acgtggacac ggaccagatc atccccaagc aattcctaaa gaaaattgaa
     5461 cgttctggat ttggaattca tctgtttcac gactggagat tctcagacgc agacgggaag
     5521 gttcccattg ctgattttct tttgaatcgc gcaccttaca ataaagcctc gattctgctt
     5581 gcgagagaga actttggctg cggttcaagc agggagcatg caccctgggc tctcgaagat
     5641 tttggaattc gctccatcat cgctccttct ttcgcagaca tcttctacaa caactgcttc
     5701 aagaatggaa tgcttcctgt gatccttccg acgggccagg tggacgaact ctttgagtat
     5761 gaagcaaaga atccggggca gtcttttgaa attgatctgc ctgcacagtt gatccgtcgt
     5821 ccggacggaa agacgtattc ctttgaagtg gatgctttta gaaaggaatg cctgctcaac
     5881 ggctgggatg acattggttt gtctttgcgg catgcagata agatctcatc ctttgaaaag
     5941 tcttacttt
//
LOCUS       DAORFF010000194         5941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7160_length_5941_cov_3.460754, whole genome shotgun sequence.
ACCESSION   DAORFF010000194 DAORFF010000000
VERSION     DAORFF010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7160_length_5941_cov_3.460754"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="PKK76_09190"
     CDS             <1..1382
                     /locus_tag="PKK76_09190"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL68690.1"
                     /translation="KLRSMYSEIRNLNESLEERVRSRTEELKKTLDDVQALKVQQDGD
                     YFLTSLLLEPLASNNSSGSTVGVQFLVHQMKHFTFRKWTKDIGGDICIADRFELKGLP
                     HTVVMNADAMGKSIQGAGGALVLGSVFHAILSRTRIVAGVQNLTPEAWLKSAFIELQK
                     VFESFGGSMLASLVLSLVDEDTGLMYHLNAEHPGLVIYRGGEARFIPGREIFKKIGTN
                     TGHDLGVITVDTFQLRPGDVVIAGSDGRDDLHVASAEEGGRIINEDMNAFLTSVEEGD
                     GELDAIYRSLQRRGSFIDDVSLVRFAFKEDAAAEIQTDTTPVSEALSALRGRGDLRQA
                     AAKAERLLIDHPHPVLYRQLLKTYIKLKEYAAACVPGQWLCLHHPRDLQSIFLASYAM
                     KRTGEYMAAAEQGERIRTRDPFALKNLVNLADVYRLMGNRDRAGKITAEILVIEPQNH
                     HALKLKSLL"
     gene            complement(1419..4772)
                     /locus_tag="PKK76_09195"
     CDS             complement(1419..4772)
                     /locus_tag="PKK76_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020767935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNL68691.1"
                     /translation="MTAEKPNHPEIYQPKHHIRFVTAASLFDGHDASINIFRRLLQSY
                     GAEVIHLGHNRSVQDVAEAALCEDVQGIAISSYQGGHVEYFKYLKQLLKSRGADHIKI
                     YGGGGGVIVQEEIQDLESSGITKIFSPEDGRTLGLAGIISSMLEGTDFSPKDLGIIQG
                     DSTKPEVLGRLITMIEEGENPPIEPGEKSCPVLGITGPGGAGKSSLTDELLNRFLEAN
                     PHLRICVFAVDPTRKKTGGALLGDRIRINSLSLHPDQIFFRSLATRKSGGTVSDALSR
                     ILSVFKRLPFDLIIVETSGSGQSDAEITEIADVNLYAMTPEFGAPSQLEKIAMLDFAD
                     IVALNKGDRAGAKDALMHVRKQYQRNHNRFSEDPQEMPVYTTVAARFQDSGVTQLYSA
                     LVDLIAKKTGVSFAPIDRSSPDLDTTEILPAGRIRYLSDIASTVRGYLAEGEAESNKC
                     SDLQSLMRARELSGDNAALVSALDERIAHLRSSIKPEVLEWLSKWPATREAYSGNTLT
                     YFIRDKQISQELRTTTLSGLSLPLIALPKTESSRDLVRYAYRENLPGQFPFTASVFPF
                     RKQDEDPIRMFAGEGGPERTNTRFHYLTGGETKSFVRLSTAFDSVTLYGEDPGLRPDI
                     FGKIGNSGVSVPTLDDCKRLYSGFNLLDPGTSVSMTINGPAPVILAFFFNTAIDQEVE
                     RYLRAEGKLEETRKRIGEFYSARGLSVPAYDRSLPAGHNGTGLLLGMTGERMVDPETY
                     AKIKKSVLTGLRGTVQADILKEDQAQNTCIFSTQFALKMMGDIQQFFSANGVRNFYSV
                     SISGYHIAEAGANPITQLAFTLANGFTYVEYYRKRGIRVDDFAPNLSFFFSNGLDAEY
                     SVIGRAARRIWAVAMKRLYGAGERSQKLKYHIQTSGRSLHAMEIDFNDIRTTLQALYA
                     LYDNTNSLHTNAYDEAITTPTEHSVRRAMAIQLIINKELGLNKNQNPLQGSYIIEELT
                     DLVEEAVLAEFDRLTERGGVPGAMEAMYQRAKIQEESLHYETLKHSGELPIIGVNTFQ
                     GKQTPPGETRMELIRSTDDEKNQMIERVANLSRAFPEEGKHALDRLKRAAASGENVFE
                     ELLETVKVCSLGQITGALYSIGGSYRRNM"
     gene            complement(4769..5614)
                     /locus_tag="PKK76_09200"
     CDS             complement(4769..5614)
                     /locus_tag="PKK76_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68692.1"
                     /translation="MKGPGLLLALLFASALSAQTERTVTILAIADWKGDFAVDQKGRG
                     GLAAFLALKRRIEQKIAPRGGSVIAVHAGQFTQATLSTALEDSLKRPDPGLIQYVGFS
                     ALTFSKQERALLEKTSLDLPVVERECTPKAKCKTEIITANSTLKWWITGLDPLKDRKS
                     DALLRDLESSAHKADADFSILLLSEPQTEAIFAGQTGNVHSAVGELGRFLVIESGKKN
                     LFYRDPAGPYVCIVAGRSICRVDLTFRGSLIGVSQKFIDPNGASEPGEFIKPDPVLMD
                     QFFRP"
     gene            complement(5673..>5941)
                     /locus_tag="PKK76_09205"
     CDS             complement(5673..>5941)
                     /locus_tag="PKK76_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL68693.1"
                     /translation="KEPVIVIEVLDGETDSVRQLKEASRAKFDEAQKLYLSQKFENAR
                     SLFIEVLKSNPDDRAAGIFVRRCRLYMEKGVPEGWNGVEQMTTK"
BASE COUNT         1333 a         1420 c         1569 g         1619 t
ORIGIN      
        1 agaaactgcg ctctatgtac agtgagattc gaaatctcaa cgagtctcta gaagaacgcg
       61 tccgatcgcg cacggaagag ctaaagaaga ctctcgatga tgtgcaggcg ctgaaagtgc
      121 agcaggacgg agactacttc ctcacatccc ttcttcttga acctcttgct tctaataatt
      181 caagcggttc tactgttggc gtgcagtttc tggttcatca gatgaagcat ttcacgtttc
      241 gcaaatggac caaggacatt ggcggtgata tttgcattgc agatcgattt gaactgaagg
      301 gattgccgca cacagttgtc atgaatgcgg atgcgatggg taaatccatt cagggagcag
      361 gcggtgctct tgttcttggt tctgtcttcc atgcaatctt gagtcgaacg cgcattgttg
      421 cgggcgtcca gaatcttacc ccggaagcat ggttgaagag tgcgttcatc gaattgcaga
      481 aagttttcga aagctttggt ggttctatgc ttgcttctct cgttctctcc ctcgtggacg
      541 aagacacagg tctgatgtat catctaaatg cagaacatcc gggtcttgtc atctatcgcg
      601 gtggagaagc tcgattcatt cctggtcgag aaatattcaa gaaaattgga acgaacactg
      661 gacatgatct gggtgtcatc actgtggata cttttcagct gcggcccgga gatgtagtga
      721 tcgcaggttc cgacggtcgc gacgatcttc atgttgcgag tgcggaggaa ggcggcagga
      781 tcatcaatga agacatgaat gcctttctta caagcgtaga agaaggcgac ggtgagctgg
      841 atgccattta tcgctctttg caacgccgag gaagttttat cgatgacgtt tctctggttc
      901 gctttgcgtt taaggaagac gcggctgctg aaatacaaac agacactaca cctgtttcag
      961 aagcattgtc cgcgcttcgc gggcgtgggg atttaagaca ggcggcagcg aaagcggaga
     1021 ggttgctcat cgatcatccg catccagttt tgtacagaca gcttttgaag acctacataa
     1081 aactgaagga atacgcggcg gcctgcgtgc ccggtcagtg gctctgtctg catcatccgc
     1141 gtgatctaca gtctatattc cttgcgtcct atgcgatgaa acgcacgggc gaatacatgg
     1201 ctgcggcaga gcagggagag aggattagaa cgcgagatcc atttgctctc aaaaacctgg
     1261 tgaatcttgc agacgtttac agattgatgg gaaatcgcga tcgtgcggga aaaatcacgg
     1321 cagaaattct agtcatcgag cctcagaatc accatgcatt gaaactgaag tctctgctct
     1381 aagcttgcgc gaagctttgc cctggaattc gttttgcgtt acatgtttcg acggtagctt
     1441 ccgccaatgc tgtagagcgc gcccgtgatc tgtcccagcg aacaaacctt cactgtttcg
     1501 aggagctctt cgaatacgtt ttcgcctgag gctgctgcgc gcttgagccg gtcgagagcg
     1561 tgtttgcctt cttccggaaa ggcgcgcgaa agattcgcca ctctctcaat catctgattc
     1621 ttttcgtcat ccgtggagcg gatgagttcc atcctggttt ctccaggtgg agtctgtttt
     1681 ccctgaaatg tatttacgcc aatgattggc aattcgcctg aatgcttgag cgtctcataa
     1741 tggagagact cttcctggat cttcgcacgc tgatacatgg cctccatggc tcctggaact
     1801 ccacctcttt cagtgagtct gtcaaattca gcaagcacag cttcttccac tagatccgtc
     1861 agttcttcaa tgatgtagga tccctggagt ggattctgat ttttgttcag tcccagttct
     1921 ttgttgatga tcagttgaat cgccatggcc cgccgaactg aatgttctgt gggagtggtg
     1981 attgcttcat cgtatgcatt ggtatggagg ctgttcgtat tgtcatagag tgcatagagg
     2041 gcttgcaggg tggttcgaat atcattgaag tcaatttcca tcgcatgcag agatcttccg
     2101 ctcgtctgga tgtgatactt cagtttctgc gacctttctc ccgcgccgta gagacgcttc
     2161 atcgccacag cccagattct tctggctgca cggccaatca ccgaatactc agcgtccagt
     2221 ccgttagaga agaagaagct gaggttcggt gcgaagtcgt ctacccggat gccgcgcttt
     2281 ctatagtatt caacgtaggt aaaaccattc gcaagtgtga atgcaagctg cgtgattgga
     2341 tttgcgcctg cttctgcgat gtgataaccg gaaatagaaa cagagtaaaa gtttcgcact
     2401 ccgtttgcgg agaagaactg ctgaatgtct cccatcatct tgagtgcaaa ctgtgtggag
     2461 aaaatacagg tgttctgtgc ctggtcttct ttgagtatgt ccgcctgaac ggttccgcga
     2521 agtcctgtaa gcacagattt tttgatcttc gcataggttt caggatccac cattctttct
     2581 cctgtcattc ccagtagaag tccggttcca ttgtggcctg cgggaagaga gcgatcgtag
     2641 gcgggcacag atagcccccg ggcagaatag aattcgccaa tccgctttct ggtctcttcc
     2701 agtttccctt ctgcgcgcag gtatctttcc acttcctgat caatggcagt attgaagaag
     2761 aatgccagaa tcacaggtgc aggtccgttg attgtcatgc ttacactggt tccaggatca
     2821 agcagattga aaccggaata gaggcgtttg caatcgtcca gagtaggaac gctgactccg
     2881 gagttgccaa tttttccaaa gatatccggc ctgagccctg gatcttcgcc gtataacgtt
     2941 acagaatcaa aagctgtgga gaggcgaaca aatgattttg tctcccctcc agtcagatag
     3001 tgaaatcgag tgttggtacg ctcgggtccg ccttcgcctg cgaacatgcg aattggatcc
     3061 tcatcctgct tgcgaaaggg gaaaacagat gccgtgaatg gaaactggcc cggaaggttt
     3121 tctctgtatg catatcgcac gaggtcgcgg cttgattccg tttttggcag agcaatcaag
     3181 gggagggaaa gaccggatag agtggtagtc cggagttcct gggaaatttg cttatctctg
     3241 atgaaatacg tgagggtgtt tccagaatag gcttcgcgcg tggcaggcca tttagaaagc
     3301 cattcgagca cttccggctt gatgctggat ctgagatgtg cgattctttc gtcgagtgcg
     3361 gatacaagtg ccgcgttgtc tcctgaaagt tcgcgcgcgc gcatgagact ctggaggtca
     3421 gaacatttgt tgctctctgc ttctccttcc gcgaggtagc cgcgcactgt acttgcgatg
     3481 tcagacagat agcggattct accagcgggg aggatttcag ttgtatcgag atccgggctc
     3541 gatcgatcaa tgggagcaaa ggaaactcca gttttttttg caatgaggtc tacgagtgcg
     3601 gagtaaagct gtgtaactcc actgtcctga aagcgtgcag caactgtagt gtacactggc
     3661 atttcctgcg gatcttcaga gaatcgattg tgattccttt gatactgctt ccttacatgc
     3721 atcagagcgt ctttcgcgcc agctcgatct cctttgttca gcgccacgat gtccgcgaaa
     3781 tcgagcatcg caattttctc tagctgagaa ggcgcgccga attcaggcgt catcgcgtac
     3841 agatttacgt ccgcgatttc agtgatttcc gcatctgatt gcccggaacc agaagtctct
     3901 acaatgatca ggtcgaaagg gagtctcttg aaaacggaaa ggattctgct gagagcatcg
     3961 ctgactgtac cacctgattt tctcgtcgca agggagcgga agaatatctg atctggatga
     4021 agcgaaagag aattgattcg aattctatct ccgagcaatg ctccgccagt tttctttctg
     4081 gttggatcta ctgcgaatac gcagatgcga aggtgagggt tggcttcaag gaatcggttg
     4141 agaagctcat ctgtgagaga cgatttgcca gcgccacccg ggccagtaat cccaaggaca
     4201 ggacagctct tttcgcccgg ctcgatcggg ggattctcac cttcttctat cattgtaatg
     4261 aggcgaccga gtacctccgg ctttgtactg tctccctgga tgatgcccag atcttttggt
     4321 gagaaatctg ttccttcgag catcgacgaa atgattccgg caaggcccag agtgcgtcca
     4381 tcttcaggag aaaagatctt tgtgattccg gaagattcca gatcctggat ttcttcctgg
     4441 acaatgacgc cgcctccgcc tccgtaaatc ttgatgtgat ctgctccgcg gcttttcaat
     4501 aattgtttaa gatatttaaa gtattcaacg tggcctccct gatagctgga gatggcaatg
     4561 ccctgtacgt cttcgcacag agctgcttcc gctacatcct gcacagagcg attgtgcccc
     4621 agatgaatga cttcagcgcc gtaggactgc agtagtctgc ggaagatgtt gatggatgcg
     4681 tcgtgcccgt cgaacaggga agctgctgtt acaaaccgaa tatgatgttt cggttgatag
     4741 atttctggat ggtttggttt ttctgctgtc atggcctgaa gaattgatcc atgagtactg
     4801 gatctggttt gataaattcg cctggttcag aggcgccgtt tggatcgatg aatttctgac
     4861 tgactccgat gagacttcca cggaatgtaa ggtccactct acaaatgctt ctgccggcaa
     4921 caatgcacac gtaaggtccg gcaggatcgc gatagaatag atttttcttt cctgattcga
     4981 tgaccaggaa tcggccaagt tcacctaccg ctgagtgcac gtttccggtc tggcccgcga
     5041 agattgcttc cgtctgtggt tcggagagaa gcaaaatcga aaagtctgcg tccgctttgt
     5101 gagcgctgga ttcgagatcg cgcaacagcg cgtctgattt cctgtctttg agaggatcca
     5161 gtcccgtgat ccaccacttg agagtggagt ttgcagtgat gatttccgtt ttgcattttg
     5221 cttttggagt gcattcgcgt tctacaacgg gcagatccag gctcgttttt tcaagcagag
     5281 ctctttcttg ctttgagaat gtaagcgcgg agaatcctac gtactggatg agtcctggat
     5341 cgggtctttt gagagaatct tccagtgcag tgctgagagt ggcctgtgtg aattgtcctg
     5401 cgtgcactgc gatgacagat ccgccgcgcg gtgcgatctt ctgttcaatt cttcttttga
     5461 gtgcgaggaa cgcggccagc cctccgcgtc ctttctggtc gactgcgaag tctcctttcc
     5521 agtccgcgat ggcaagtatc gtgaccgttc gctcagtttg cgcggagagt gcggatgcaa
     5581 acaaaagcgc cagcagcaat ccgggtcctt tcatggttca cagtcagttg ttttggacag
     5641 ggtgcaagca actttcgaaa cgtcagcgaa gcctatttcg tggtcatttg ctctacgcca
     5701 ttccagcctt cgggcacgcc cttctccatg tacaatctgc atcgcctcac gaagattccg
     5761 gcggcgcgat catcggggtt gcttttcaat acttcaatga aaagagaacg agcgttttca
     5821 aatttctggc tgaggtacaa tttctgggct tcatcaaatt tcgcgcgcga agcttccttt
     5881 agctggcgga ctgaatctgt ttctccgtcg agcacttcga ttacaatgac cggttccttt
     5941 t
//
LOCUS       DAORFF010000195         5933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7181_length_5933_cov_3.197516, whole genome shotgun sequence.
ACCESSION   DAORFF010000195 DAORFF010000000
VERSION     DAORFF010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7181_length_5933_cov_3.197516"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..2408
                     /locus_tag="PKK76_09210"
     CDS             87..2408
                     /locus_tag="PKK76_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNL68694.1"
                     /translation="MIQSFQKFFRRSHPGSILLLILVLPLKIFPETLAGDPVDLLRAG
                     RWGELQRYFRSNRASSDTHRYILGRAMQEKIHKIPISPETAPLAESLREPVSIYLGLL
                     GLTCDLSSQMKLNDCIHDLPNESVTSQIPRLAILKLSAMVHATGWNDARLRLLSKSAL
                     REPDPLTAKIYTEKLEVLLAKQMNAEALELAGRLGGVSQDKTHFYRARAYIRAGRRDD
                     GWNLYLLAAARSLDDPGLLRSIFQDLKYYFPAAFRAGSDQHSLHRSLFMFYDFLTPQE
                     KAALASAWPAPRLLETTSPQTTLGDGNHLISIGDRASFQDLAKRSYTAVSREPLILYK
                     WGWKLTAARRYAETLSLIDQFDFVKKENAGIWRLQLDTLEAMGDKDRAFRETLAYLAV
                     FDYDLEVHDSLIEFLIGSDDKHIHWAKPAYWELARGSMPPGSANGRFVYWLRRMYLAT
                     GETEKAKDVEKKFYEMAPGSYYARAFWDEMQPGDFRRDWQDVRDRPTYLRWIGRHGGN
                     PDAIRFLAARNLSSYFDPRATALWQRLQSVQYSVPEPVVTVWKLGEFDLGAEYFDYYF
                     KDRMSRKELFLRKVMLGLKSGHLDQSVWHLRQLARDENIPEDPFSIAPDFLKQLYPRP
                     YLREVRSASSEFGLSEDMIYALMRQESMFRELAVSRSGALGLMQVMPATGRWLAPRMQ
                     MKNPDMLEPSVSIRMGGKFFADLLRSYDNDFRWASIAYNGGPGSLARWKAQYYHGDFN
                     LFLENIPSKESRNYCRVTFQNYMHYRTTYTLYP"
     gene            2439..3977
                     /gene="cysS"
                     /locus_tag="PKK76_09215"
     CDS             2439..3977
                     /gene="cysS"
                     /locus_tag="PKK76_09215"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNL68695.1"
                     /translation="MSFRLFNSLSGKKEEFQPGQPGVVKLYNCGPTVYNFNHIGNFRS
                     YMSVDVLRRSLLFFGYKVDHTTNITDVEDKIIANAAKQGKDIFAFTKPFIEYFLEDLA
                     YLGIQDVEHRPKATDSIPAMIDLMQKLEKSGHTYSQDGDVYFRLKSFSEYGKLSRIEP
                     EQLKAAAGGRFTADEYTKEDIRDFALWKKQQAPSEPAWDSPYGKGRPGWHLECSAMIR
                     DIYGAGGVDIHAGGIDLLFPHHENEIAQSCCAYRGENFVRYWVHNEHLLVDGKKMSKS
                     LGNYFTLRDLSEKQGLERLISENRAPADTMKLHEKLSMKRALRYLLLSTHYRSRLNFT
                     FENLKASDAACERIQTCLDKALGTSGITEKQAAATFAARDPAQPQKLFTDKGLATAIQ
                     SFQDALLDDLNISRALAAVFDAVSDLNSRGMDADLARQAVVFFYAANQIIAVLDFRPE
                     DQREKPQADLGDLASYVQSKIEERLRARAEKNWALSDQIRDELKAKGIILKDSPQGTT
                     WEKA"
     gene            3974..4720
                     /locus_tag="PKK76_09220"
     CDS             3974..4720
                     /locus_tag="PKK76_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNL68696.1"
                     /translation="MRDNIVYGRQTVREFLRADNPPRVNKIYLSESFPNDLRSQVMAL
                     GGAVQELPRREIDRMFPGIHHQGVVLELAGSYRAAAPATAKDALALGGPFLALDEVSD
                     PQNLGSILRTAEALGVRAVFSGSKTSPVTPAVHRASSGASLHIPAYNVGNLPQFVERM
                     KEAGIWIIASVPDETEPARGQTFLRTTDSDDLPEAEKLCLLVGSEGTGIKSILIERAD
                     YLMTIPMHGKTGSLNAGVACAIILERIVNR"
     gene            complement(4722..5564)
                     /locus_tag="PKK76_09225"
     CDS             complement(4722..5564)
                     /locus_tag="PKK76_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004479792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNL68697.1"
                     /translation="MNPQLLDGKAASEQIRNDLRSRVEKLKLRGGPVPVLVTILVGNN
                     PSSEVYVKMKGKACESIGLGSRKIQMPEETTTEELLKVIDECNADPTVRGILLQHPSP
                     SQIDERAAFDRIAIDKDVDGVTAFGFGRMSLGIQAYPACTPAGIVKLLDHYKINLSGL
                     HAVIVGRSPILGKPMAQLLLNRDCTITVCHSRTKDLPRHLQSADLVVAACGKPRYIVG
                     DWIKEGAIVVDAGYNDGNIGDADFESCAKKASWITPVPGGVGPMTITTLIAHTVESAE
                     KASG"
     gene            complement(5545..>5933)
                     /locus_tag="PKK76_09230"
     CDS             complement(5545..>5933)
                     /locus_tag="PKK76_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68698.1"
                     /translation="LDMLKFRFFSFLFLAAVASGMFLANCGPARDPIPTGITTAEMGA
                     GQIRVTATGRAEQGTIDRANGAMMRTTSCDAARLLLKNELKSTAYKNPSRNFKESSEV
                     HLIYNGEYCRLSGIYDPAGKFEPTTP"
BASE COUNT         1525 a         1570 c         1439 g         1399 t
ORIGIN      
        1 agaacgcacg gatgggcctg gaaaacagtt cttcccagca tacccgggcc aagaaaactc
       61 ggtaagaaga acgaatcatg ggttgcatga tccaatcttt ccaaaaattc ttccgccggt
      121 cgcatccagg ctctatcctg cttctcatcc tggttttgcc actgaaaatt ttcccggaaa
      181 ccctggccgg ggatcccgtg gatctcctgc gcgccggccg ctggggagaa ctccagcgtt
      241 acttccgttc caatcgagct tcttccgaca cccacagata cattctcggc cgggccatgc
      301 aggaaaaaat acacaagatt cctatcagcc cggaaaccgc gccgcttgca gaatctcttc
      361 gggaacctgt ttcgatctac ctcggactcc tgggccttac ctgtgacctt tccagtcaga
      421 tgaagttgaa tgattgcatc cacgatcttc caaacgaaag cgtgacgtcc cagattccac
      481 ggctcgccat actcaagcta tccgcaatgg tccacgcaac tggatggaat gacgcgaggc
      541 tcaggctact gagcaaatcc gcgctcagag aaccggaccc actcacagcg aagatctaca
      601 ccgaaaagct agaggtgctt cttgccaaac aaatgaatgc agaggcactc gagcttgctg
      661 gacgactggg tggtgtttcg caggacaaga ctcatttcta tcgcgccaga gcctacattc
      721 gcgcaggaag aagagacgat ggctggaatt tgtatttgct tgcggctgct cgaagcctcg
      781 atgatccggg actcctcagg tccatcttcc aggatttgaa atactatttt cccgcagcct
      841 ttcgcgctgg atcggatcaa cacagccttc atcgttctct attcatgttc tatgatttcc
      901 tgactccaca ggaaaaagca gcactcgctt ccgcctggcc tgcccccaga ctcctcgaga
      961 ccacatctcc tcagaccact ctgggagatg gcaatcatct catctcgatt ggagatcgcg
     1021 cttcttttca ggatcttgca aagagaagct acactgctgt ttcgcgtgaa cccttgattt
     1081 tgtacaaatg gggatggaaa ctgaccgcgg ccagacgtta tgcggaaact ctttccctca
     1141 tagatcaatt tgattttgtg aaaaaagaaa acgcaggaat atggagactg cagctggata
     1201 ctctggaagc aatgggagac aaagaccgtg cattcagaga gactcttgcc tatctcgcag
     1261 ttttcgatta cgatctggaa gtgcacgaca gcctgatcga atttctgatc ggcagcgacg
     1321 acaaacacat tcactgggcc aaacctgcct actgggaact cgcacgcgga agcatgcctc
     1381 ccggaagtgc gaatggtaga ttcgtttact ggcttcgcag aatgtatctg gccaccggag
     1441 aaactgaaaa agcaaaagat gtagaaaaga aattttatga aatggcacct ggttcgtatt
     1501 acgctcgtgc gttctgggac gaaatgcagc caggcgactt tcgccgcgac tggcaggacg
     1561 taagagaccg gcctacatac ttgcgctgga taggcaggca cggaggaaat ccagacgcca
     1621 ttcgcttcct ggctgcgcgc aacttatcga gttactttga tccgcgagcg actgcgctat
     1681 ggcaaagact gcaatccgtt cagtattccg tgcccgagcc tgttgttaca gtctggaagc
     1741 tcggagaatt tgacctgggt gcagagtact ttgactacta cttcaaagat cgtatgtcca
     1801 gaaaggaact cttcctgaga aaggtcatgc ttggcttgaa atccggtcat ctggatcaat
     1861 ctgtatggca cctgcgtcag cttgcacggg atgaaaacat tccagaagat cctttttcta
     1921 tcgcgccgga ttttctcaag cagctctacc ctcgcccgta tttgcgcgaa gtgagatccg
     1981 cctcctccga gttcggtctt tccgaagaca tgatctacgc actgatgaga caggaatcca
     2041 tgtttcgcga actggcagtc agcagatccg gagcgctcgg tctgatgcag gtcatgcccg
     2101 ccactggcag gtggcttgcg cccaggatgc agatgaagaa cccggacatg cttgagccat
     2161 ccgtatccat tcgcatgggt ggcaaattct ttgcggatct tcttcgctcc tacgacaatg
     2221 acttccgctg ggcgtcgatc gcctacaatg gcggacctgg aagtctggcc agatggaagg
     2281 cacagtacta tcacggtgat ttcaatctgt tcctcgagaa tattccttcc aaagaatccc
     2341 gtaactactg ccgcgtgacg ttccagaatt acatgcatta tcgcactacc tacacgctgt
     2401 atccttgacg aatgacgctc cacgacgccc atgaccatat gtctttccgt ctattcaact
     2461 ctctgagcgg aaaaaaagaa gagttccaac ctggccagcc tggtgtagtg aaactctaca
     2521 actgcggtcc gacggtttac aatttcaacc acatcggcaa cttcagatcc tacatgtctg
     2581 tggatgtgct tcgcaggtcc ctgctcttct ttggatacaa agtggatcac acaaccaaca
     2641 tcacagatgt ggaagacaag atcatcgcga atgcagcaaa gcagggaaaa gacatcttcg
     2701 ctttcacaaa gcctttcatt gaatatttcc tggaagatct agcatacctg ggcattcagg
     2761 atgtagagca ccgaccaaag gcaaccgatt ccatccccgc gatgatagat ctcatgcaga
     2821 aactggaaaa aagcgggcac acctattctc aggatggaga tgtctatttc agactgaaat
     2881 ctttctcgga gtacgggaag ctttcgcgca tcgaaccgga acaactgaaa gcagctgcgg
     2941 gcggaagatt cacagcagac gaatatacaa aagaagatat aagagacttc gcactctgga
     3001 aaaaacaaca agcgccttct gaaccagcct gggattcacc gtatggaaag ggcagacctg
     3061 gatggcacct ggaatgctct gccatgatcc gagatatata cggagccggt ggtgtagaca
     3121 tacatgcagg tggaattgat ttattgtttc cacaccacga aaacgaaatc gctcaatcct
     3181 gctgcgcata ccgcggagaa aacttcgtac gctactgggt gcacaacgag catctccttg
     3241 tggacggaaa gaagatgtcc aaatctctgg gcaactactt cacccttcgc gatctgagcg
     3301 aaaagcaagg actcgaaaga ctcatctctg aaaatcgcgc tcccgcagat acaatgaagc
     3361 tgcatgaaaa gctctccatg aaacgcgctc tacgttatct gctcttgtct actcactatc
     3421 gctctcgctt gaatttcact ttcgaaaacc tcaaagcatc agatgcagcg tgtgaaagaa
     3481 tccagacctg tctggacaaa gctctcggca cttccgggat cactgaaaaa caagcggccg
     3541 caaccttcgc agcgcgcgat ccagcgcagc ctcaaaaact gttcacggac aaaggcctcg
     3601 ctactgcaat ccaatctttc caggatgcac ttctcgacga tctgaacatt tcacgcgccc
     3661 tggccgctgt ctttgatgct gtaagcgacc tgaattcaag aggcatggat gcagatctgg
     3721 ccagacaggc agttgtgttt ttctacgcag ccaaccagat catcgcagtg cttgattttc
     3781 gcccggaaga ccagcgcgaa aaaccacagg cggatctggg agatcttgca tcttacgtgc
     3841 aaagcaagat cgaagaaaga ctccgtgctc gcgcggaaaa aaactgggca ctttccgatc
     3901 agatcagaga cgaactcaaa gcaaagggaa tcatcttgaa ggattcacct cagggaacca
     3961 cctgggagaa ggcttgagag acaacatcgt ttacggcaga caaactgtgc gcgagttttt
     4021 gcgcgcagac aatccgccgc gagtgaataa aatctatttg tcagaaagtt ttccaaacga
     4081 tctcagatcg caggtcatgg cactgggcgg tgcagttcag gaattgccaa gaagagaaat
     4141 agacagaatg tttcctggca ttcaccacca gggagtggtg cttgagctgg caggttctta
     4201 cagagcagcc gctcctgcga cagcaaaaga cgcactcgct ctgggcggac ctttcctggc
     4261 gctcgacgaa gtttcagatc cacagaacct gggaagcatc ctgcgcactg cagaagctct
     4321 cggagtgcgc gctgtttttt caggaagcaa aacctcccct gtcactccag cagttcacag
     4381 agcatcgagc ggtgcaagcc ttcacattcc ggcatacaat gttggaaatc ttcctcaatt
     4441 tgtagaaaga atgaaagaag cagggatctg gatcatcgcc tcagttccgg atgaaacaga
     4501 accggccaga ggacaaacat tccttcgcac aaccgattcg gatgatcttc ctgaagcgga
     4561 gaaactgtgc ctgcttgtgg gaagtgaagg aacaggaatc aaatcgatct tgattgagcg
     4621 cgcagattat cttatgacca ttccaatgca cggcaaaaca ggttcactga acgcaggcgt
     4681 ggcctgtgcg atcattcttg agagaatcgt aaaccgctga tttaaccgga agctttttct
     4741 gcgctttcta ctgtgtgcgc gatgagagtg gtgatggtca tggggccgac tccgcctgga
     4801 accggagtga tccagctagc tttcttcgca cagctttcga aatcagcgtc accaatgttt
     4861 ccgtcgttgt agcctgcatc caccacgatg gctccttctt tgatccaatc acctacgatg
     4921 taacgcggct ttccacatgc tgccacaaca aggtccgcgc tctgcaggtg acgcggaaga
     4981 tcttttgtgc gggaatgaca cacagtgatg gtgcaatccc gattcagtag aagttgtgcc
     5041 attggttttc ctagaatcgg tgatcttcca acgatgacag cgtgtagccc actcagattg
     5101 atcttgtaat gatcgagcag cttcacaatt cctgcggggg tgcaggcagg gtaagcttga
     5161 attccaagac tcattctgcc aaaaccaaat gcagtgactc catctacgtc tttgtctatt
     5221 gcgattctgt cgaatgctgc acgctcatca atttgagaag gagaaggatg ctgcagcaat
     5281 attccgcgga cggttggatc cgcattgcac tcatcgatta ctttcagtag ttcttctgta
     5341 gtggtctctt ctggcatctg gatcttgcgc gatccaaggc caatcgattc gcaggccttt
     5401 cctttcatct tcacgtaaac ttctgaagat ggattgtttc caacgaggat ggtcacgagc
     5461 accggaactg gaccgcctcg cagcttcagc ttttctacgc gagagcgaag atcatttcgg
     5521 atttgttctg acgcagcttt gccatcaagg agttgtgggt tcaaattttc ctgccggatc
     5581 gtagattccg ctgagtctgc aatactcgcc gttgtaaatc aagtgcactt cgctactttc
     5641 tttgaaattc ctggacggat ttttgtatgc agtacttttc aattcatttt tcagaagaag
     5701 cctggccgca tcgcagctgg ttgtgcgcat catcgcacca ttcgcacgat caatcgttcc
     5761 ctgctccgca cgtcctgtcg cagttactcg aatctggccc gctcccatct ctgcagtggt
     5821 gatcccggtt gggatgggat cacgcgcagg accgcagttt gcgagaaaca ttccagaggc
     5881 aaccgcggcc agaaacagaa aactaaagaa tctaaacttt aacatatcca aat
//
LOCUS       DAORFF010000196         5913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7220_length_5913_cov_3.639809, whole genome shotgun sequence.
ACCESSION   DAORFF010000196 DAORFF010000000
VERSION     DAORFF010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7220_length_5913_cov_3.639809"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PKK76_09235"
     CDS             complement(<1..965)
                     /locus_tag="PKK76_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68699.1"
                     /translation="MSRAKSILFLTLLPSVIVLVLFAYTYAKKWTQKDEIVATATDGT
                     QIHGIRAMSGAVSGSSQSLAVFLPDPAFDRSLNSRSIETNAGAELEALVLKRGIHLLR
                     YDSRGTGRTPGVSRFVPPGILADDLMKAGAGATTVIAHGDSCATAALAWKKGMHAKRW
                     IFVSCAYSGNPVDEWASRTFYSMENSLMSEKLIQTCRKEWSAFRKDLRQTVSSKSGPP
                     PVVPAKEGESPDLMVFRGAMREMAYDQKAWTREALDFDVMRTIVQMNQKEDIVWFVPE
                     FDTVTVPSEVEAMKKAGLTPRPLFQTDYFLLQTDSPRRTSMERIF"
     gene            complement(966..2057)
                     /locus_tag="PKK76_09240"
     CDS             complement(966..2057)
                     /locus_tag="PKK76_09240"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNL68700.1"
                     /translation="MKLKLFFFPIVLFLGAFILDKQLFIGRFEDTFLRTGTFLNFDHK
                     VDMLDELAAYLIAPARKKTLVIFGNSRTMSFENSYIESKYKDWIWFNFSVPGGTTDYF
                     LFLIQEMKRKGIRPDAVVLTVTPQGFNTAPAVPMDEVLISGLSFPFIARYANRYTTDD
                     LMNYAAKKTFWTYRYRPKAEVILHRLEHDGLQMRIFRQFQFDTATRLKVSRGSVPFQF
                     QGIPAQDDEFLMAHAKNTWQDFFTPYKLSEGQFAFTDDSLRILTDARIPVILLWARVQ
                     KNLRQMKDTVVVTPRGEKPETVRSLWAPRMTALAAKYQVPFYDMNYGNSIACDHFFDS
                     SHLASVCFPEFSDYIMRAVLDQKPEGQKP"
     gene            complement(2065..3591)
                     /locus_tag="PKK76_09245"
     CDS             complement(2065..3591)
                     /locus_tag="PKK76_09245"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNL68701.1"
                     /translation="MLFNSFLFLIFFGFVYILYWALRGRARIAFLIGASVLFYSAWGL
                     QSEGWSGLRWTLHFVVITFLNYLLIHAMFRYPLRKKSIITAIIVIDILNLATFKYFAF
                     FRDSLHNLSLRLPEKLDALNLFLPLAISFYTFQLIAYAMDVYRGVIKEKVPVGRFFLF
                     FLFFPHFIAGPIMRASDLMPQIDKPWVDRNRMMGACWLILGGLVKKVLLADPMGQIVA
                     PVFREPDAYSGLSIILGGACFSLQVYCDFSGYTDIARGCAFLLGFDIPENFSAPFFAR
                     SARELWQRWHITLATWLRDYLYIPLGGSRRSEVRNHLNLIITFTLGGFWHGADISYIL
                     WGFFWGALLSLERFLENNMGWKTTPEKSRAGMVLKGMFMFLLFSIGALFFRTQPVRFA
                     DHSYTTWQMLGHVFGGIFTNTDGELLSTIRAAGMDPAQIGMIFGPSALELRRIGPMDT
                     IIWMFLALGFFHWVQYSPARFARFKEQPWLLGAVAALLGGILIPSLVSGTGQFIYFVF
                     "
     gene            complement(3604..4590)
                     /locus_tag="PKK76_09250"
     CDS             complement(3604..4590)
                     /locus_tag="PKK76_09250"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNL68702.1"
                     /translation="MRDPALSVLLERVLEEDKLLLTTHRTPDPDGLGAELAMQAMLQW
                     RGKDAIIVNPEPAPERIRFVDPERRALDMRNMKDRSLLDGRAIVILDNSDLRRIDDLQ
                     SYVLEDKSNLIIIDHHDGMQADQKSIFAFPNCGSSCEIVYELLEIAGFTMNPAIAAAL
                     YAGIVADTGNFRYRKTTARTHEIAAKLLRFGVNPADMAEKIFENTPLARLLLKKKLYS
                     NLIIEQGRLAYFKVRKEDLEALGLSMEDTEGLVNELIESESILTGILFTERQPQLTKV
                     SVRSRGDTNMLAAVQKFGGGGHKNACGATLYMDIDAAVKEFVPEALRCLDEC"
     gene            complement(4587..>5913)
                     /locus_tag="PKK76_09255"
     CDS             complement(4587..>5913)
                     /locus_tag="PKK76_09255"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNL68703.1"
                     /translation="KRNVSALHRYLQVHIFQYNGMQAPSSESHSALLAQELHMPGLLC
                     GNSLRYIRANLPVLVFHCTDDELAKARITTQAELPGLEKISIQGSAVHLIHRKNGAEH
                     RWIFRKPEYFSTIDTLVYQAGVWSGSVEAMEDLALGRIRLAPGVPLDACHAIRLAAEA
                     ALLDFQIEEELLGAAKQAIASREEEPTDEFLGRVFRDVVSGAKPSTGFVALRDMGALS
                     LMVPELAEGQNLSQNQYHEFDIFFHSIFACDALKEQNLALRLAALLHDVGKSKTRRVG
                     SDGQATFHNHEVVGARQTDRLLRRLGFESDLIARVRFLVRHHMFHYTPDWTDHAVRRF
                     MRKVNVDQMRDLIAVRIADRKGSGKRAGVPRPVYDFLAHMERIRAEDAQLKVTDLTLG
                     GNELMEMGIPAGRGMGDVLKRLLEEVKSGALVNEKEALSARVREYGGVH"
BASE COUNT         1584 a         1565 c         1393 g         1371 t
ORIGIN      
        1 aagaaaattc gttccatgga tgtacggcgc ggactgtccg tttgcagtaa gaaataatcc
       61 gtttgaaaga gcggcctggg agtcagcccg gcctttttca ttgcctccac ttctgacgga
      121 acagtaaccg tgtcaaactc tggcacaaac caaacaatgt cttctttctg attcatctga
      181 acgattgtgc gcataacgtc aaaatccaag gcttcgcgcg tccatgcctt ctgatcatat
      241 gccatctctc tcatggcacc tcgaaatacc atcaagtccg gtgactctcc ctcttttgcc
      301 ggcacaaccg gcggcggacc agatttgctt gaaacagtct ggcgaagatc ctttctaaaa
      361 gcagaccact ctttacggca tgtctgaatc agcttttccg acatgaggct attttccata
      421 ctatagaagg tgcgagaggc ccattcatcc acagggttgc cactgtacgc gcacgacaca
      481 aagatccatc tcttcgcgtg cattcctttc ttccatgcaa gcgccgccgt ggcgcatgaa
      541 tccccatgag cgatgaccgt tgtagcaccg gcccccgcct tcatcaaatc atccgcaaga
      601 atcccgggag gaacaaatcg cgagactcca ggagttctac cggtgccgcg cgaatcgtag
      661 cgcaataaat gaattcccct ctttagaacg agagcttcca attctgcgcc cgcattcgtt
      721 tcaatggagc gactgttcaa ggaacgatca aaggcaggat ccggcaagaa cacagcgaga
      781 gactgcgaag agccagagac cgcaccggac atggcgcgaa tgccgtgaat ttgtgttccg
      841 tctgtggctg tcgcaacaat ttcatccttc tgagtccatt tcttcgcgta cgtatacgca
      901 aagagaacga gcacaatcac ggacggcaga agcgtcaaaa agagaatgga ctttgcgcga
      961 ctcattcagg gcttttgtcc ctccggcttc tggtccagaa cagcgcgcat gatataatca
     1021 gagaattccg gaaagcacac ggatgcgaga tgactggaat caaagaaatg atcgcatgca
     1081 atggaattgc cgtagttcat atcatagaac ggcacctgat actttgccgc gagcgctgtc
     1141 atgcgaggtg cccacagaga acgaaccgtc tctggcttct ctccacgcgg agtgaccaca
     1201 acggtgtctt tcatctgcct tagattcttt tgcacgcggg cccagagcaa aatcaccggg
     1261 attcgagcgt ccgtcaaaat gcgaagagaa tcatccgtaa aagcaaattg tccctctgaa
     1321 agcttgtagg gagtaaaaaa atcctgccag gtattcttcg catgggccat caagaactcg
     1381 tcatcctgcg ccggaattcc ctggaactga aatggaacgg atcccctgct taccttcaaa
     1441 cgagtggcgg tatcaaattg aaactgtctg aaaatcctca tctgtagtcc atcgtgctcg
     1501 aggcgatgca ggatcacttc cgcctttggt cgataacgat atgtccagaa tgtcttcttg
     1561 gccgcgtaat tcatcaaatc gtctgttgtg tagcgattgg cgtatcgcgc gatgaacggg
     1621 aaagataaac cactgatcaa cacttcgtcc atgggcacag caggagcggt gttgaaacct
     1681 tgaggagtta cagtcaatac gactgcatcc ggtcttattc cttttctttt catttcctga
     1741 atcaaaaaca ggaagtagtc tgtggtccca ccaggtacgg agaaattgaa ccagatccag
     1801 tctttgtatt tggattcgat gtagctgttc tcgaaagaca ttgtgcgaga atttccaaaa
     1861 atcacgagag tcttctttct agcgggcgca atcaggtatg cggccagctc atcgagcata
     1921 tccactttgt gatcaaagtt cagaaatgtt cctgttctca gaaacgtatc ttcgaaccgt
     1981 ccgataaaca gctgtttgtc gaggataaac gctccgagga aaagtacgat agggaaaaag
     2041 aataatttca gcttcataaa tccctcagaa tacaaaatag ataaactgcc ccgtcccgct
     2101 caccagagac ggaataagaa ttccaccgag caaggccgcc accgcgccca gaagccaggg
     2161 ctgttcttta aagcgtgcaa aacgcgccgg agaatactgc acccagtgaa agaatcccag
     2221 agcaagaaac atccagatga ttgtgtccat cgggccaatg cgacgcaatt cgagcgcgga
     2281 aggaccaaag atcattccaa tttgagccgg atccattccc gcagcacgaa ttgtggatag
     2341 aagttcgccg tctgtatttg tgaaaatccc tccaaataca tgtccaagca tttgccacgt
     2401 tgtgtagctg tgatccgcga aacgaacggg ctgcgtgcga aagaaaagcg ctccaatgga
     2461 aaacaaaagg aacatgaaca ttcctttcaa aaccataccc gcgcgcgatt tctccggagt
     2521 agtcttccag cccatgttgt tttcgaggaa gcgctccaga gacaacagcg caccccagaa
     2581 gaatccccag agaatgtagc tgatatctgc gccgtgccag aaaccaccca gagtaaaagt
     2641 aataatcaaa ttcagatgat ttcttacttc actgcgtcta cttccaccca gaggaatgta
     2701 gagataatct cgcaaccacg tagccagtgt tatgtgccag cgctgccaga gttctctggc
     2761 acttctcgca aagaaaggcg ctgaaaaatt ttccggtata tcaaacccaa gaaggaaagc
     2821 gcaaccgcga gctatatctg tgtatcctga gaaatcacag tagacctgca aagaaaagca
     2881 agcaccgccc agaatgatgg aaaggccgga gtaagcatct ggctctcgaa atacaggcgc
     2941 gacgatctga cccattggat ctgcaagcag caccttcttc acaagaccgc ccagaatcag
     3001 ccagcaggcc cccatcatgc gattgcgatc cacccagggc ttatcaattt gcggcatcag
     3061 atccgatgca cgcataatgg gtccggcaat gaagtgagga aaaaacaaaa agaacaaaaa
     3121 gaatctgccc accggcactt tctctttgat cactccgcga tacacgtcca tcgcatacgc
     3181 aatcagctga aaggtgtaga aggaaatcgc gaggggaaga aacagattca gtgcatccag
     3241 cttttctgga agtctaagag aaaggttgtg gagtgaatct cgaaagaatg cgaagtactt
     3301 gaacgtagcc aggttcaaga tgtcgattac gatgatcgcg gtgatgatgc tcttcttacg
     3361 aagagggtat ctgaacatcg cgtgaatcaa caaatagttc aggaaagtga tgactacgaa
     3421 atgtaaggtc cagcgaagac cactccagcc ttcactctgc aaaccccacg cagaataaaa
     3481 aagaacggat gcaccaatta gaaacgcgat tcgggcacga ccgcgcaaag cccagtatag
     3541 aatgtagacg aagccaaaaa atatcagaaa taaaaacgaa ttaaagagca tcgcaggtca
     3601 aaatcagcat tcgtccagac aacgcaacgc ttctggaaca aattccttca ccgccgcatc
     3661 gatgtccata taaagcgtcg caccgcacgc attcttgtgc ccgccgccgc caaacttctg
     3721 cacggctgcc agcatgtttg tatctccgcg cgatcgcacg gagactttgg tcaactgagg
     3781 ctggcgctct gtaaaaagaa tcccggtcaa aatgctttcc gattcaatca attcattgac
     3841 caggccttcc gtatcttcca tgctcaagcc gagcgcctca aggtcttctt tcctcacttt
     3901 aaaataagca agccttccct gttcgatgat tagattgctg tagagtttct tctttaagag
     3961 aagtcgcgca agcggagtat tctcaaaaat cttttcagcc atgtccgcag gattgacacc
     4021 gaaacgaaga agcttggccg cgatttcgtg tgtgcgcgct gtagtcttgc gatatctgaa
     4081 attccccgta tccgcaacaa ttccggcgta gagcgcggca gcaatggccg ggttcatggt
     4141 gaatccagca atctcaagca actcataaac gatttcgcag cttgatccgc aattgggaaa
     4201 agcgaagatt gatttttggt ccgcttgcat tccatcatga tgatcgatga tgattagatt
     4261 ggacttatcc tccagaacat agctctgcaa atcgtcaatg cgcctcaggt cagaattgtc
     4321 cagaatcaca atggcccgac cgtcgagcaa cgaacgatcc ttcatgtttc gcatatcgag
     4381 tgctctgcgc tccgggtcta caaagcggat tcgttccggc gctggttctg gattcactat
     4441 gatcgcatct tttccgcgcc actgcagcat tgcctgcatg gccagttccg ctcccaatcc
     4501 atctggatca ggagttctgt gcgtggtcag aagaagcttg tcttcttcca ggacccgttc
     4561 gagcaatacg gaaagagcag gatctctcaa tggacgcctc cgtattctct tactcgcgcg
     4621 ctgagggctt ctttttcgtt tacaagcgcc cccgacttca cttcctcgag gagccttttc
     4681 agcacgtctc ccattccgcg acctgcagga attcccatct ccatcagttc atttccgcct
     4741 aacgttaagt ccgtgacttt gagctgtgcg tcctctgcac gaattcgctc catatgggca
     4801 aggaagtcat acacgggtct tggtacgcct gcccttttac cacttccttt gcgatccgct
     4861 atgcgaactg caatcagatc ccgcatttga tccacattca cctttctcat gaatcgcctg
     4921 accgcatggt ccgtccaatc cggagtgtag tgaaacatat gatgacgcac aaggaaacgc
     4981 actctcgcga tcaaatcaga ctcaaatcca agacgacgaa gcaatcgatc tgtttgcctg
     5041 gcgcctacta cttcatgatt gtgaaacgtt gcctgcccat cggagccaac gcgacgagtt
     5101 tttgattttc ctacatcgtg caagagggct gccaggcgca gtgcgaggtt ctgttctttg
     5161 agcgcgtcgc atgcgaaaat actatggaag aatatatcga attcgtggta ttgattctgc
     5221 gaaagattct gcccttccgc gagctcagga accataagag aaagagctcc catatcacgc
     5281 agcgcaacaa atccggtgga aggctttgct cccgacacaa cgtcgcgaaa gactctgcca
     5341 agaaattcgt ccgtcggttc ttcttcgcgc gatgcgattg cctgctttgc tgccccgagc
     5401 agttcttcct cgatttgaaa atccaggaga gccgcttccg ctgcaaggcg aatcgcatga
     5461 catgcgtcga gaggtacgcc tggcgcaaga cgaatccgcc ccagtgccag gtcttccatt
     5521 gcctcgacag atccgctcca aacgccggct tgataaacca gagtgtctat tgtggagaaa
     5581 tactcaggct tgcgaaagat ccatctgtgc tctgcgccat tcttgcgatg aatcaaatga
     5641 acagctgagc cctggatgga aattttttcc agcccgggaa gttccgcctg ggtagtgatc
     5701 ctcgctttcg cgagttcgtc atccgtgcag tggaaaacga gtacgggcag gtttgcgcga
     5761 atatagcgaa ggctgttccc gcaaagcaaa ccgggcatat gtagctcctg ggccagtaga
     5821 gctgagtggc tttctgaaga tggagcctgc ataccgttgt actgaaagat atgaacctgc
     5881 aagtaccggt gtaaagcaga aacgttgcgc ttg
//
LOCUS       DAORFF010000197         5911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7229_length_5911_cov_4.109802, whole genome shotgun sequence.
ACCESSION   DAORFF010000197 DAORFF010000000
VERSION     DAORFF010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7229_length_5911_cov_4.109802"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PKK76_09260"
     CDS             <1..652
                     /locus_tag="PKK76_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68704.1"
                     /translation="LIGLVLFAALVVFPLMLMALLFPVQIQDTRPQLPFPNPLFGPLS
                     RFVLPIVFILLSAGFLLYERRPLPFAWPESVGISARLTSGDRKFLLQQSSVGGDSRFA
                     VYGLGDSSLILKRDLFAPGTAHDPHICFEAAGFAFQGETEETIGDIRLKSALVQKDGK
                     SARLFWWYSWNTKRSSSDLEWRVARLRGEDVIQFNLYGFDDAALRNAAQMLLKSEI"
     gene            668..2392
                     /locus_tag="PKK76_09265"
     CDS             668..2392
                     /locus_tag="PKK76_09265"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HNL68705.1"
                     /translation="MENQMTFARRSAWFLGIVSVFCAGLFQIPVNLQGTLVYSATLVV
                     FWWRIRHSKVRSFWQHPLIAVVGGFILAGFLTFTTNGLLVTALHLSIFYLLGDLLLRQ
                     FGEGPPESISDYCGRAAKLILLSLKRFFSRSGVVEFSSLKTLREDGTRGRLPLVLFSV
                     LLGFVIFFLLHYLFAAVNDDYRRFMDPLVRWVWSACLKNLCLSVIHAYILFCIFTVER
                     SENKKEERALPIFTAVAITVGAVIVTGVFAFFQTRVVLSSSDLNFHELSKYTQKGFLQ
                     LIFAILLGYLIALLAIYSKQKAGKGNHLIALVIFLMLEMILAASFSAHKLYLLQSAFG
                     LKDQRILASCGVLFVGFSFLLLFWKIFSPNPNFSGFRAQTFFLGALVVALNWINVDLF
                     TTHTNPIRYYVDKKPYVDYSYLLSNSDDNFAEWPALMRRMKEENPPAPPHGYFWGFES
                     KSGKDAFYIRYAPICSDRPEIRTVKEADGTTLHLPTAHVAFLFKKYGTLAPAEPAKNP
                     VASPNNELDEEDIQPEEIHPSFDLSQALDFNWREYASYKSARANAKTFEEFSLYLDKY
                     CEAPPGRM"
     gene            complement(2476..2856)
                     /locus_tag="PKK76_09270"
     CDS             complement(2476..2856)
                     /locus_tag="PKK76_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNL68706.1"
                     /translation="MLSNTVLWSLQGVLCFVFLAAGLLKMLRTREQIMNGGGEWAVNV
                     SARNIRIIGVLEIAAAIGIILPLYFNVMPVLTFFAAVGMIFTMIVAGSLHFKRGEMAL
                     LLVTVATGFFAALVAFGRRGLLGL"
     gene            2900..4714
                     /locus_tag="PKK76_09275"
     CDS             2900..4714
                     /locus_tag="PKK76_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68707.1"
                     /translation="MNGGDIVGKKNHLRILNIRNGTTFALLALVTTTWYFAAGLLSPS
                     WRDDSLGGNDWDLNFFYTESSALPVRDFAQIPFWNPFYKGGMPLFENPQARTFTPAVF
                     LAPVMGPVRALKFSVFIYFLLGAAGSFYLFRFALSIRSLALVIPVVMVPFCGYVPQHV
                     FAGHANFVSILMLPWCLGAMILFQNTGRFRHVAVFSLSAATMVWDGHIYGFLQLLVFV
                     TIVFSTMAVVQKKPEIFLNAFILLVAACALSAVRLLPEIVYFLKYGAYFRPDHVSLTL
                     SNVLHTFVASSQDPLLARNWENQEHAWWEYGNYIGLLPFLLLLCALPFLRKSEVPWLV
                     ALVLFILLMMGDFHVLSPAHLLSQIPGFSNMRCSGRWGFPVVISLAVLLSICLHRALE
                     KASEYTGMGAIASVLVICFSLYFCYDIRKNRKPISQTFVSGKSASSLVSRIERSLITV
                     PSLPTYGANSAQFAGILNFFSVRDGYDMLNSNTSMPAKGEAFYRGEYYLEHGSEVKPQ
                     LWTPNRIDFEIESEKGDQLVINQNYYSDWRATDGFEAKSHNGIISVLLHAGKNRFSIY
                     YLSTAAVVGFLLGMAMFVGVYFFCEFRTRRFIPEQNPA"
     gene            complement(4689..>5911)
                     /locus_tag="PKK76_09280"
     CDS             complement(4689..>5911)
                     /locus_tag="PKK76_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNL68708.1"
                     /translation="AAAYSDLSSVMEEFAASIDMSNSATESQIELIDQVSAQASEVEL
                     LLRSIRGAGEELQAQIEEARTGGESVTRSAQNLSQAMDDIEKAFATVSQVTRIMTEVS
                     DRTNLLSLNASIEAARSGEHGRGFAVVAEEISKLAEKSAANAKQIASVISGNASAIST
                     GHKSANVAESKVQEQRSVFLSIAERYGQVTENVRNQIGKSEELVGRIRSLRELSSEIS
                     GAAREQKAGVDSAMKSLLSMESSTSSLVSDANFIEMTAKLIEDQAQSLQSEYHEDLRK
                     TEARLATDIRSSLDRLPFDSDVDLWMEQATAVVPSAAFRLYLCNSAGTQLSPNFLRKG
                     NAFVAQPEYRGSNWSERAYFAENMSRVARSGGASMSAKYIDRETEQETITLTLQVAPD
                     AFLFIDLKPDSAQG"
BASE COUNT         1264 a         1424 c         1490 g         1733 t
ORIGIN      
        1 actgattggt ctggttttgt tcgcggcact ggttgtgttt cctctcatgt tgatggctct
       61 gctctttcca gtccaaatac aagacactcg accacagctc cctttcccca atcctttgtt
      121 cgggcccctg agtcgattcg tgcttcccat tgtttttatt ctcctgtcag ctggctttct
      181 tctatacgag aggcgcccac ttccgttcgc ctggcctgag tcggtaggga tcagtgcaag
      241 gctgacttcc ggagatcgga aatttctttt gcagcagagc tctgtggggg gagattctcg
      301 ttttgctgtc tatggccttg gcgactcgtc attgattctg aagagggact tgtttgcgcc
      361 cggcactgcg catgatccgc acatttgttt tgaggcagca ggtttcgcat ttcaaggaga
      421 aacagaagag acgataggcg acattcgctt gaagtccgcc cttgtgcaga aagatggcaa
      481 atctgccaga cttttctggt ggtactcctg gaacacgaaa cgttcttctt ctgatctgga
      541 atggcgcgtc gcacggcttc gcggagagga cgtcattcaa ttcaatctgt atggttttga
      601 cgacgcggct ctgcgaaacg ccgctcaaat gcttcttaaa tccgaaatct agatggagaa
      661 gaacagtatg gaaaatcaaa tgacttttgc acgccggtct gcctggtttc ttggaatcgt
      721 ctcagtgttc tgtgcgggcc ttttccagat cccggtcaat ttgcaaggaa ccctggtata
      781 ttctgcgact ctggttgttt tctggtggag aatcagacac tcgaaagttc gttccttctg
      841 gcagcaccca ttgatcgcag tggtgggcgg attcattctg gcaggattcc tgaccttcac
      901 cacgaacggt ttgctagtga ctgctctgca tctgagtatc ttctacctgt taggcgatct
      961 tttgctgcgt cagtttggag agggcccacc ggaaagcatt tccgactact gtgggcgggc
     1021 agcaaagctc atcctccttt ccttaaagag atttttttcg cgcagtggcg tggttgaatt
     1081 ctcttctctg aagactttgc gcgaagatgg gactcgcggt agactgcctc tcgttttgtt
     1141 ttcagtcctg ctgggcttcg tgatcttttt tctgttgcat tacttgtttg ccgcggtcaa
     1201 tgacgattac agaagattca tggatccact tgtgcgctgg gtatggtctg cgtgtctgaa
     1261 gaatctttgc ctctccgtga ttcacgccta cattctgttt tgtatcttca ctgtagagag
     1321 atcggagaat aaaaaagaag agcgggcatt gccgatcttt accgccgttg caatcacagt
     1381 gggggccgtg attgtgactg gagtgtttgc tttttttcag acgagggtag tgctttcttc
     1441 gagtgacctg aattttcacg aactctcgaa gtacactcag aagggatttc tccagttgat
     1501 tttcgcgatc ctgctgggat atttgattgc acttcttgcc atctatagca agcaaaaagc
     1561 aggcaaggga aatcatctca ttgctcttgt gatttttctg atgctggaga tgattctggc
     1621 ggccagtttc tcagctcaca aactgtactt gcttcaatct gcatttggtt tgaaagacca
     1681 gagaatactt gcgagctgcg gcgttctgtt tgttggtttt tcttttttgc ttctattctg
     1741 gaagatattc tctcctaatc ctaatttttc gggcttccgg gctcaaacgt tctttctggg
     1801 tgcgctcgtg gttgcgctca actggatcaa cgtggatttg ttcaccactc atacgaatcc
     1861 gattcgctac tatgtggata aaaagcctta tgtagattat tcttacttgc tttctaattc
     1921 cgacgacaac tttgccgaat ggccggcctt gatgcggagg atgaaagagg agaatcctcc
     1981 tgctccgcct cacggatatt tctggggctt tgaaagcaaa tccgggaaag atgcattcta
     2041 tattcgctat gctccgattt gttctgatcg acctgaaata cggacagtaa aggaggcgga
     2101 cggtacaacg ttgcatttgc caacggcgca tgtggctttt ctcttcaaga aatatgggac
     2161 gcttgctccg gccgaacctg cgaagaatcc tgtcgcatca cctaacaatg agttagacga
     2221 agaggatatt caaccagagg agattcatcc ttcgtttgat ctgagtcagg ctcttgattt
     2281 taactggcgc gaatacgcat cgtacaagag tgcgcgggcc aatgcgaaga cgtttgaaga
     2341 gtttagcctc tatctggaca aatattgcga ggcaccaccg ggacgaatgt agtcccggcg
     2401 gggtttgtct attctggaat agtcggacct ttggctccaa atgcccagga cgcatgtagt
     2461 cccgcatgcg ctgcgttaca ggcccaggag tcctcgccga ccaaaggcca caagtgccgc
     2521 aaaaaatccg gtggctacag tgaccagtag tagggccatt tctccgcgtt tgaagtggag
     2581 agatccagca acgatcatgg tgaaaatcat acctacggcc gcgaagaagg tgagtacggg
     2641 catgacgttg aagtagagcg gcagaatgat tccgatggca gctgcgattt ccaggacacc
     2701 gatgatgcga atgtttcttg cggaaacgtt tacggcccat tctcccccgc cattcatgat
     2761 ttgttctctg gttcgaagca ttttcaagag gcctgccgcc agaaaaacaa aacacaaaac
     2821 tccctgaagt gaccacagaa cggtgtttga aagcatgtcg gattgtagcg cacgtggctg
     2881 agacggtcaa cgaaaatcct tgaatggtgg ggacatcgta ggaaagaaga atcacttgag
     2941 aatcctgaac attagaaacg gcaccacatt cgcgctcctt gcgcttgtga ctacgacgtg
     3001 gtattttgcc gccggtcttc tttcgccttc gtggagggat gattccctgg gtggcaatga
     3061 ctgggatttg aatttctttt acacggagag ttctgctctc cctgtccgag atttcgcaca
     3121 gattcctttc tggaaccctt tctacaaggg cgggatgcct ttgtttgaga atccgcaagc
     3181 gcgtacattt acgcctgctg ttttcctggc cccggtgatg ggccccgtgc gtgctctgaa
     3241 gttttccgtt ttcatctact tcctgttagg tgcagcgggc agtttctact tgtttcgttt
     3301 tgcattgtcc attcgaagcc ttgcgctcgt cattcctgtt gtgatggtcc ctttttgcgg
     3361 atacgttccg cagcatgtgt ttgccgggca tgcgaatttc gtttcgatcc tgatgcttcc
     3421 ctggtgtttg ggtgccatga ttctgtttca aaacacaggt cgatttcgtc atgtagcggt
     3481 tttttctctc agtgcggcga ctatggtctg ggatggacac atttatggat ttcttcagct
     3541 gcttgtattt gtgacgatcg tgttttctac gatggccgtt gttcaaaaaa aacctgaaat
     3601 cttcctgaac gcatttattt tgctggtcgc agcatgcgcg ctttcggctg tgaggttact
     3661 tccggaaata gtatattttc taaagtatgg tgcttatttc aggcctgacc atgtttcact
     3721 cacactttcg aacgtgttgc ataccttcgt ggcctcttcc caggatcctc ttcttgcgcg
     3781 aaactgggaa aatcaggaac acgcgtggtg ggaatatgga aactatattg gcctcttgcc
     3841 tttcctgttg ctactatgcg cacttccctt tttgcgaaaa tcagaagtcc cctggttggt
     3901 ggcactggta ttgttcatcc ttctgatgat gggcgatttt cacgttcttt cgcccgcgca
     3961 tttactctcg cagatcccag ggttcagcaa tatgcgatgc tcaggacgat ggggtttccc
     4021 tgttgtcatt tcgctggctg tcttactttc catctgtctc catcgagctc tggaaaaagc
     4081 aagcgagtac actggcatgg gagcgattgc cagcgtgctg gtgatatgct tttcgttgta
     4141 cttctgctat gacatacgca agaacagaaa gcccatttcg caaaccttcg tatccggaaa
     4201 atccgcatcc agtctggtgt ctcgaatcga aagatctcta attacagtac cctctcttcc
     4261 aacatatgga gcaaactccg cgcagtttgc aggcatcttg aatttctttt ctgttcgaga
     4321 tggttacgac atgctcaatt caaacacgtc catgccggca aagggagagg cgttttatcg
     4381 aggggagtac tatctggaac acggctcaga agtaaagccg cagctctgga ctccaaacag
     4441 aatcgacttt gaaattgaaa gcgaaaaggg agaccagctt gtcatcaatc aaaattatta
     4501 ttctgattgg agagcgacag acggatttga agcaaagtcg cataacggta tcatttccgt
     4561 gcttttgcat gccgggaaaa ataggttttc catctactat ctgagtacgg cggcagtcgt
     4621 tgggtttctt ctgggaatgg caatgtttgt gggcgtgtac ttcttctgtg agtttcgcac
     4681 gagaagattc atccctgagc agaatccggc ttgaggtcaa tgaatagaaa tgcgtctggc
     4741 gccacctgga gagtgagcgt tattgtttct tgctctgttt ctctatctat gtatttcgct
     4801 gacatggatg ccccgccact tctggccacg cgtgacatgt tttctgcgaa gtaggcccgc
     4861 tcgctccagt tggatccccg atactctggc tgcgcgacga atgcatttcc ctttctaaga
     4921 aagttgggtg acaactgtgt tcccgcggag ttgcaaaggt aaagacgaaa cgcggccgac
     4981 ggaacaacag ctgttgcttg ctccatccag aggtcgacat ctgaatcaaa tggtagccga
     5041 tcaagactgc ttcgtatgtc tgtagccagg cgtgcttccg tttttcttaa gtcctcgtgg
     5101 tattcgctct gcagagactg cgcctggtct tcaatgagtt tagctgtcat ttcaatgaaa
     5161 ttcgcatcgc ttacgagcga gcttgtagaa gactccatgg acaaaaggga tttcatcgcg
     5221 gagtctacgc cggccttttg ttctctggca gcaccgctaa tttcagatga aagctcgcgc
     5281 agactacgaa tacgacctac aagctcttca cttttgccga tttgattgcg cacgttttct
     5341 gtgacctgac cgtatctttc cgcaattgaa agaaacacgc ttctctgttc ctggactttg
     5401 ctttccgcaa cgttagccga cttatgcccg gtggagatag cagatgcatt tccagaaata
     5461 acggaggcga tttgtttcgc attggcagcg cttttttctg cgagcttgct gatttcttct
     5521 gcaacgacgg cgaatcctct tccatgctct cctgagcgtg cggcttcaat ggaggcgttc
     5581 aatgagagca ggttggtgcg atcagacact tcagtcatga tccgcgtcac ctgcgaaaca
     5641 gttgcgaatg ccttttcaat atcgtccatg gcctggctca agttttgcgc ggatcgagtg
     5701 accgattcgc cacctgtgcg ggcttcttcg atctgggcct gcaattcttc accggctcct
     5761 cgaatgctgc gaagtagtag ttccacttcg gatgcttgag cgctcacctg gtctatcaat
     5821 tcgatctggc tttctgtagc ggagttggac atatcaatgg atgccgcgaa ttcttccatg
     5881 acagagctca ggtccgaata ggccgccgca t
//
LOCUS       DAORFF010000198         5908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7236_length_5908_cov_3.701521, whole genome shotgun sequence.
ACCESSION   DAORFF010000198 DAORFF010000000
VERSION     DAORFF010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7236_length_5908_cov_3.701521"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1346
                     /locus_tag="PKK76_09285"
     CDS             219..1346
                     /locus_tag="PKK76_09285"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL68709.1"
                     /translation="MMKSRLSILLCSLFSLCSIQCSAAFLPGQQQLDGRVLAVWLIHP
                     PWNYSRFLFAVSALDTTMRSYRIDSATGALTQVTAFPTISGIGIDIHPTLSYVYLANN
                     TTDGVIAAQFDTAGALTSAGSINTGNSNTHRVKVHPAGNAIYFNSVQAGNTRMFRAPL
                     NSGSMGTAVLQNASEPSNSQFMTLDPTGRFLYGTISGTGGVNTFTTNGGASLTAQGNT
                     LISGTEEPVRAQFLSDGSGMFLYTTSSGRLMSYSINATTGMPIPVSNISVNVGALFIH
                     PNQGYLYAGDTASGGLRLYRINGDKSLTFVNYYTGMGGVSYDNGCFERGGQFLFLTKA
                     NATGVIQVVKINADLSPTLASSLTTGGNLGECTTSIDSYWP"
     gene            1472..1544
                     /locus_tag="PKK76_09290"
     tRNA            1472..1544
                     /locus_tag="PKK76_09290"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Thr,seq:ggt)
     gene            1549..1620
                     /locus_tag="PKK76_09295"
     tRNA            1549..1620
                     /locus_tag="PKK76_09295"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1582..1584,aa:Trp,seq:cca)
     gene            1643..1828
                     /gene="secE"
                     /locus_tag="PKK76_09300"
     CDS             1643..1828
                     /gene="secE"
                     /locus_tag="PKK76_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNL68710.1"
                     /translation="MLKFLKESREELRKVVWPSKEEVMNSTLVVLGAVVLISLFLFSI
                     DRMFSAMFDALIRLGSG"
     gene            1828..2361
                     /gene="nusG"
                     /locus_tag="PKK76_09305"
     CDS             1828..2361
                     /gene="nusG"
                     /locus_tag="PKK76_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNL68711.1"
                     /translation="MMSWYVIRTYSGHENKVKTNLEKLVQTRNLAGKVGQINIPTIKV
                     AEMKAGKKRVVEKKFMPGYILAELEMDDELQYTIRKLPSVAGFVGSPDPQALTADEVK
                     NLMSSEGAVEEEQPRARMLFSVGETVKIVDGPFANFNGVVDEIMPDKGRLRVKVEIFG
                     QATPVELDYLQVAANVR"
     gene            2388..2816
                     /gene="rplK"
                     /locus_tag="PKK76_09310"
     CDS             2388..2816
                     /gene="rplK"
                     /locus_tag="PKK76_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNL68712.1"
                     /translation="MAAKKIVTQVKLQVEAGKATPAPPVGPALGQHGLNIMEFCKQFN
                     ERTKNQVGIKIPVVITVFSDRSFTFITKSPPAALLIKKELGLESASATPNKAKVGTIK
                     RAQLEKIAEIKMKDLNCHDMDAAVKILAGTCRSMGVNVEG"
     gene            2818..3504
                     /gene="rplA"
                     /locus_tag="PKK76_09315"
     CDS             2818..3504
                     /gene="rplA"
                     /locus_tag="PKK76_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNL68713.1"
                     /translation="MKRGKKYKAVVEKVERTRHYEPTEAAALMKEVSTSKFDGTVEAS
                     VKIGYKSLQNVRGIVRLPHGTGKEVRVLVFAKEDKQAEAKAAGADFVGGTEMVEKILK
                     EGWTDFDACVATPDLMKDVGKLGAILGRKGLMPKPKAGTVTTDVGTAVKNLKSGQMEY
                     RPDKTGVIHVRIGRVSFTPDKIEENMRAVFAAITRDRPSDAKGEYVRSFYVSPTMGPA
                     ARVNIRAMAT"
     gene            3516..4064
                     /gene="rplJ"
                     /locus_tag="PKK76_09320"
     CDS             3516..4064
                     /gene="rplJ"
                     /locus_tag="PKK76_09320"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNL68714.1"
                     /translation="MPAHATVISPENKESVTKLKEILSKHANFVVTTYSGLSVVDMGE
                     LRSAVREKDGRVKVVKNNLFRIALKESAVHASAADAISDKLKGPIAVTFAGADISAIS
                     KALVDFSKKNNKVEVITGFLEGKKLDKAEVQQIANLPSREQLLTIIGRGLNTPATKIA
                     IGMKEVITSLARGIRAVGEKNG"
     gene            4102..4485
                     /gene="rplL"
                     /locus_tag="PKK76_09325"
     CDS             4102..4485
                     /gene="rplL"
                     /locus_tag="PKK76_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNL68715.1"
                     /translation="MSTDALLESIGKLTLMEAAELVKKMEEKFGISTAAPVAVAAGPA
                     AGGAADAGAGEDATFNVVLKSHGEKKIDVIKVVREVTGLGLKEAKDLVEKAGSVVKEG
                     LAKAAAEDLKKKLEALGAQIELKAG"
     gene            4577..>5908
                     /locus_tag="PKK76_09330"
     CDS             4577..>5908
                     /locus_tag="PKK76_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNL68716.1"
                     /translation="MAQNKYVRLGTITDYDQIPDLIDIQFKSYDWFLQKDAGEAKRKS
                     QGLDAVFQETFPIESPNEDMVLEYVSYSLGDPRWDEEECKNRGTTYALPLKAIIRLIN
                     KLTGEVREQSVYMGDLPVMTEQGTFVINGAERVVVSQLHRSPGIFFFYDAEKRVYSAR
                     VIPYRGSWLELELDTKGILVARIDRKKKFPATLLLKSMGYGSDEDVLKLFYKIESLKL
                     AGLTGRDLKKIIGRRLAARVLNPENDEVIMYAGEKVNEDNLDILKEMKIKELKLLVFP
                     TSKDDVIVINCLEKEGTETQEAALQKFHTLMRPGEPFNPENAKADLNRLFFSEKSYDL
                     GGVGRYKINNKFAYHNPKEFTGIDTRALRPEDIVETMRYFVNLINEVPEYHFDDIDHL
                     GNRRVRSVGELLMNQLKVGFSRMERVVKERMTVQDIDVITPQALISIKPIMA"
BASE COUNT         1588 a         1414 c         1532 g         1374 t
ORIGIN      
        1 tcctggctgg gcgaaacctc ggttggtgga tcatcggggc gtccatcttc gcctgcctgg
       61 ccctgctcct ggctgttccg gatccttcca gaaaagcttc tttgaagcag actctggatt
      121 cttatctaca gaggcttgcg gcaatttgat atagaagtaa tcgcttgcgc ttgctcgatc
      181 cggggtgatt ttattactta agaagcaagg aacataagat gatgaagtcg cgtttgagca
      241 tactattgtg ttcgcttttt tctctctgtt ccatccaatg ctctgctgca tttctaccag
      301 ggcagcaaca gctcgacggg cgcgtcctgg ctgtgtggct cattcatccg ccctggaatt
      361 acagtaggtt tttgttcgca gtgtccgcgc tcgatacaac catgcgttcg tatcgcattg
      421 attccgcaac aggcgcactg actcaggtta ctgcctttcc tacgatctcg gggatcggaa
      481 tcgacattca tcccacgctg tcctatgttt atcttgcaaa caatacaacg gacggagtaa
      541 tcgcggcgca atttgatact gcgggcgcgt tgacttctgc gggttccatc aatacgggga
      601 attcgaacac tcaccgcgtt aaagtccatc cggcaggaaa tgcaatttac ttcaactcgg
      661 tccaggcagg aaacactcgc atgttccgcg ctcctttgaa ttcgggaagc atggggactg
      721 cggttttaca gaacgcaagc gagccttcca acagtcagtt catgactctg gatccaaccg
      781 gtagattcct ttacggcacg atcagcggaa cgggcggcgt aaatacattc actacaaatg
      841 gaggagcaag tctgactgct cagggaaaca ctctcatttc cggcacagaa gaaccggttc
      901 gcgctcagtt tttgtcagac gggtcgggca tgttcctgta taccacttcg agcggccgat
      961 tgatgtctta ttccatcaat gcaaccaccg gtatgcctat tccagtttcc aacatatcag
     1021 tcaatgtggg tgcgcttttc atccatccta accagggata tctgtatgcc ggggacactg
     1081 caagcggggg actccgcctc tacagaatca atggggataa atcgctcact ttcgtgaact
     1141 actacaccgg gatgggtggc gtgagttacg acaacgggtg ctttgagaga ggaggccaat
     1201 tccttttcct tacgaaggca aatgcgactg gtgtaatcca ggttgtgaaa atcaatgcag
     1261 acctgagccc gactctggca tccagcctga caacgggagg caacctcgga gaatgcacaa
     1321 cgtctattga ctcttactgg ccctgaggca tctgcctttc gcattcattc gccggcagga
     1381 tgaacgcaaa ttccattcca cttgacatta gacgtacaga taaattttat ggacattctc
     1441 ccgaacaggt atgtccggat cgggggcggg tgccctctta gctcagttgg tagagcgcat
     1501 ccatggtaag gatgaggtca ccagttcaag cctggtagag ggctcaggag gccggtagtt
     1561 caatggctag aataggggtc tccaaaaccc ttgatgagag ttcgattctc tcccggcctg
     1621 agggaaccag gaaacgtaga gtatgttgaa gtttctaaaa gaaagtcgcg aagaactcag
     1681 gaaggttgtt tggccttcca aagaagaagt tatgaattcc actctcgtcg tgctcggcgc
     1741 tgtggttttg atttctctgt ttttattttc catcgatcgt atgttttcgg caatgttcga
     1801 tgcattgatt cggttggggt ctggctaatg atgagctggt acgtcatccg gacctactcg
     1861 ggccacgaga acaaggtcaa gaccaacctc gagaagctgg tgcaaactcg caatctcgca
     1921 ggcaaggtcg gtcagatcaa catccccacc atcaaagtgg cggagatgaa ggctggcaag
     1981 aagcgagtcg ttgaaaagaa gttcatgccg ggttatattc tggctgaact cgaaatggac
     2041 gatgagctgc agtatactat tagaaagctt ccttccgttg cgggctttgt aggatcaccg
     2101 gatcctcagg cgcttacagc ggatgaagtg aagaatctga tgagttcaga gggagctgtg
     2161 gaagaagagc aaccgagagc aagaatgctt ttctcggtcg gagaaaccgt caagatcgtg
     2221 gatggtccgt ttgcaaactt caacggtgtc gtagatgaaa tcatgccgga caaaggccgc
     2281 cttcgcgtga aggtggaaat ctttggccag gccactcctg tagagctgga ttatctgcag
     2341 gtggcggcta acgttcgata gcattgtata gattatagag ggactagatg gcagcgaaga
     2401 agatagtaac acaggtcaag ttgcaggtgg aagcaggaaa agctacaccg gcgcctccgg
     2461 ttggtccggc tctgggtcag cacggcttga acattatgga attctgcaag cagttcaatg
     2521 agcgcacaaa gaatcaggtg ggaatcaaga ttcctgttgt gatcaccgtg ttctctgatc
     2581 gcagctttac tttcatcacc aagtcgcctc cggctgctct tctcatcaag aaggagctgg
     2641 gtcttgagtc tgcatctgca actcccaaca aggcgaaagt cggcacaatc aagcgtgcgc
     2701 aacttgagaa gatcgcagag atcaaaatga aggacttgaa ttgccatgac atggacgcgg
     2761 cagtcaaaat tctggcagga acatgccgat ccatgggcgt aaacgtagag ggttgagatg
     2821 aaacgcggca agaaatacaa agcagttgta gaaaaagtcg aacgcactcg tcattacgag
     2881 ccaacggaag cggcagcact catgaaagaa gtgagcactt ccaaattcga cggtacagta
     2941 gaagcatccg ttaaaatcgg atacaaatct ctgcagaacg ttcgcgggat cgtacgtctt
     3001 ccgcatggca ctggaaaaga agttcgcgtt cttgtgtttg caaaagaaga caagcaggca
     3061 gaagcaaaag cagccggtgc tgattttgtt ggcggaacag aaatggtaga gaagatcctg
     3121 aaagagggat ggaccgattt cgacgcttgc gttgctactc ccgatctcat gaaggatgta
     3181 ggtaagctcg gtgcgatcct tggtcgcaag ggcctcatgc caaagccaaa agctggaact
     3241 gtaaccacag atgttgggac agctgtgaag aatctcaaat ccggccagat ggaatatcgt
     3301 ccggacaaga ctggtgtgat tcatgttcgt attggccgcg tgtcttttac accggacaaa
     3361 attgaagaaa acatgcgcgc agtttttgct gccatcactc gtgatcgccc ttcagacgcg
     3421 aaaggcgaat atgttagaag tttctatgtg tctccaacta tgggacccgc tgcgcgtgta
     3481 aacatccgcg ctatggcgac ttaaggaatt agaaaatgcc agcacatgca actgtaatca
     3541 gtccagaaaa caaagaaagc gtaacaaagc tcaaagaaat cttgagcaag catgccaact
     3601 tcgttgtgac cacttattca ggtctcagcg ttgtggacat gggcgagttg cgctctgcag
     3661 tgcgcgaaaa ggacggccgg gtaaaggtcg tgaagaataa cctctttcgc atcgcactca
     3721 aagaatcagc ggttcacgcc agtgcggcag atgcaatcag cgacaagctc aaagggccaa
     3781 tcgctgtaac atttgcaggc gcggacattt ccgctatatc caaagctctc gtagacttct
     3841 ccaagaagaa caacaaggtg gaagtaatca ccgggtttct cgaagggaag aagctcgata
     3901 aggcggaagt tcagcagatc gccaatcttc cttctcgcga gcagctattg acaatcatcg
     3961 gacgcggcct caacacgcct gccacgaaga ttgcaatcgg catgaaagaa gtcatcacga
     4021 gtctcgctcg cgggatccgc gccgtcggag agaaaaacgg ctaacgcaaa aatttactga
     4081 aaccagggta aggagaaaac aatgtctaca gacgcactgc tcgaatcgat cgggaaactg
     4141 actcttatgg aggcagcaga gctcgttaag aaaatggagg agaagttcgg aatctccacc
     4201 gctgcaccag ttgcagtagc tgcaggtcca gccgcaggcg gagccgctga cgcgggcgca
     4261 ggcgaagatg caacattcaa cgtggttctg aagagccatg gtgagaagaa aatcgacgtg
     4321 atcaaagtcg ttcgcgaagt tacgggtctt ggtctgaaag aagccaaaga tcttgtagag
     4381 aaggctggat ccgttgttaa agaaggactt gctaaagcag cagctgaaga tctcaagaag
     4441 aagcttgagg cactcggcgc tcaaatcgag ctcaaagcag gctaattgct cgattccttt
     4501 tgttcgcagc acagcagggc atccgccctg ctgattgctt ttttcttttg tttattttcc
     4561 tgaccaggag cagcccgtgg cccagaacaa gtacgtacgt ctcggaacaa ttacagacta
     4621 cgatcaaatc ccggatttga tcgatatcca gtttaaatcc tacgactggt ttcttcaaaa
     4681 agacgctgga gaagccaaac ggaaaagtca gggcctcgac gctgtttttc aggaaacatt
     4741 cccgattgag agccccaacg aagacatggt gctcgaatat gtttcctatt ctcttggcga
     4801 tcctcgctgg gatgaagaag aatgcaaaaa tcgcggaacc acatacgctc ttcctctcaa
     4861 agcaatcatt cgtttgatca acaaactcac aggtgaagtt cgtgagcagt ctgtttatat
     4921 gggagacctt cctgtaatga cagagcaggg cacgttcgtc atcaatggcg cagagcgcgt
     4981 agttgtatca cagctccatc gcagtcctgg tattttcttt ttctacgacg cagagaagcg
     5041 cgtgtattca gcacgcgtca ttccttaccg tggcagctgg ctggaactgg aactcgacac
     5101 caaaggcatt ctggttgctc gcattgaccg taagaaaaaa ttcccggcta cactgcttct
     5161 caagtctatg ggctacggct cggatgaaga tgtactcaaa ctcttctata agattgaaag
     5221 cctgaagctc gccggtctca cagggcgcga tctcaagaaa atcatcggca gacgccttgc
     5281 agcgcgtgtt ctcaatccag agaacgatga agtgatcatg tatgcaggtg agaaagtcaa
     5341 tgaggacaac ctcgacattc tcaaagagat gaagatcaag gaactcaagc tccttgtatt
     5401 ccctaccagc aaagacgacg tcattgtcat caactgtctg gagaaagaag gaacagagac
     5461 tcaggaagcg gctctccaga agttccacac tctcatgcgt ccgggtgaac ctttcaatcc
     5521 agaaaacgca aaagcagatc tcaacagact cttcttctct gagaagtcgt atgatctagg
     5581 cggcgttggc cgttacaaga tcaacaacaa gttcgcatac cacaacccga aagaattcac
     5641 tggaatcgac acacgtgctc ttcgtccgga agacatcgta gaaaccatgc gctacttcgt
     5701 gaatttgatc aacgaagtac ctgaatatca cttcgatgac attgatcacc ttggaaatcg
     5761 ccgcgtaaga agcgtgggcg aacttttgat gaaccagttg aaagttggtt tcagcagaat
     5821 ggaacgcgtt gtcaaagagc gcatgacggt tcaagacatt gacgtcatca ctccgcaggc
     5881 tctcatttcc attaaaccga tcatggcg
//
LOCUS       DAORFF010000199         5904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7245_length_5904_cov_4.634296, whole genome shotgun sequence.
ACCESSION   DAORFF010000199 DAORFF010000000
VERSION     DAORFF010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7245_length_5904_cov_4.634296"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /locus_tag="PKK76_09335"
     CDS             complement(<1..1620)
                     /locus_tag="PKK76_09335"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNL68717.1"
                     /translation="MPAIAAVISVINGTYAFRRRSVRGALYFFLMPLGTAIWSFGYAG
                     ELLGKTQPFMTAMDNLEFIGSDLVVIGFFLFAWHYTRTTRPPLFLLAFPVINQIIAWT
                     DTGLLRHGTQVKEMGGVQFLVYDYGPYMWANIAVYYSLLLAGSFLFARRLPSVHGIFR
                     TQIVLMILSATIPWIASLFTVFGLLPIPIPNLDISPASLTVGNLLLTAALFSFGTLRI
                     SPLARERTFEMIEEGVLVCDQHGVIVDFNSSAKRLLGDKYSLEIGQRIADELARAPSE
                     YSVSDSSWLQVRRNSLLDSSGTEIGKIYTLQDVTQHRQARRALQDATTQAQNANKAKS
                     IFLANVSHEIRTPLTAILGIAEILEGTKLDEEQKRMVSSFRSAGTSLLETINGLLDLS
                     RIEAGKMQMEARVFSLHEMLENTCRMFRLQIESREEDARVELKLTIAPGVPENIKADD
                     LRLGQILTNIIGNAVKFTRVGKIDVRAVFHQGILQIVVQDTGIGIPKKKIDNIFKPFE
                     QGDVSTTRSFGGTGLGLAIVRRNIELMGGRVA"
     gene            complement(1641..2354)
                     /locus_tag="PKK76_09340"
     CDS             complement(1641..2354)
                     /locus_tag="PKK76_09340"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNL68718.1"
                     /translation="MLSRLAAGFRAYFSSLGFLRKNNLLSHIFLPALAGLLVVALLGA
                     CVIFFSGGLSESIFLRLEQFHDLPDFVRTSLRLLFIFAGLVLTVIAYRPLSSMLVLPF
                     IGPILAQVEKVLLGKEIETTVSREIKNAMIGGWLGVQASIAALFIFVITLPLGPVQAP
                     VMFLVNSYILGKSAFDFIFEKETGSVAERRAITKKYRPEILGVGIGFILFLLVPIAGA
                     ALAPIFAVVAAARIRHGSN"
     gene            complement(2354..2740)
                     /locus_tag="PKK76_09345"
     CDS             complement(2354..2740)
                     /locus_tag="PKK76_09345"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNL68719.1"
                     /translation="MLVSLCRAKIHRATVTQTELQYPGSLTLDLDLIEAAGLYPYEQV
                     SVVNINNGSRLETYIIEGERGSGMVCLNGAAARMAAKGDLVIVMAYGFFQPGEIAPDY
                     KANVVHVDTQNRITSVEHGELHGQVR"
     gene            complement(2757..3443)
                     /locus_tag="PKK76_09350"
     CDS             complement(2757..3443)
                     /locus_tag="PKK76_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNL68720.1"
                     /translation="MSRSFFELEQNIRLILNQSQELTLQSSKVNEKVEKYILHILKMI
                     LTRYDRESLLELVYTITKELAINGAKANQKRLFFDEHGLDITNERDYARGMLEFRQAF
                     SDEMNEVYGIKSREKGVYVKIYLSHQPNGLCIEVENNTAIAPAEELRLREKMKKSMSY
                     NDIAEFYMDNMDNTEGAGLGIALIMIMMKSEGLDPALFRIGTLEDRTIARVEIPFNAE
                     YVSKRSQIGR"
     gene            3513..5486
                     /locus_tag="PKK76_09355"
     CDS             3513..5486
                     /locus_tag="PKK76_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNL68721.1"
                     /translation="MLPTAQASERAALKRAIFLDPAVSLGKDLAMEIHPLDELYGSDG
                     MGAGLSLGVAIAQLATFESHRDQLWNWILSHPETELRLIVISPQDHPKSYFRPIPEEI
                     IHLVLPSNVSQQVLTDVIEGAFHVLKLHNDKVQLQSRLALSYQDIRRLTRVGQALATE
                     RNFETLIGLILQLAREIVAADAGSIYVVERPRHNEKPTHIRFKKSAMNLAADEFLLPI
                     DRNSIAGYVALTGQPLMIENVYALSGQEGYSFNYEFDRLHNYYTKSMMVIPMKNHRDE
                     VIGVIQLINKKRSSARKLTLEDMKGREVVPFTDNCRELVSALAGQAAVAIQNTILVQD
                     INNLFEGFVKASVFAIEQRDPTTSGHSFRVAEFTVGLAQAVDRLSSGKFADLKFSPDQ
                     IRELRYASLLHDFGKVGVRENVLVKAKKLYEPDMELIRWRFHYIRKALEKDVLEKKLK
                     YYKENGAVGFAQYEKYVDLEFRETMEEVNEFFRIINESNEPSVLEAGNFARLEEISQR
                     KFRVDDGSDVPFLKQNELVSLSVRKGNLDRQERLEIESHVSHTYKFLVQIPWTSELRR
                     IPDIAHGHHEKLDGSGYPLGLKSQEISPQTRMMTISDIYDALTASDRPYKKSVSQERA
                     IDILKLEVKDGHVDSDLLDIFIEAEIFKVQYRS"
     gene            complement(5490..>5904)
                     /locus_tag="PKK76_09360"
     CDS             complement(5490..>5904)
                     /locus_tag="PKK76_09360"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNL68722.1"
                     /translation="MASRARARINGKDIELVEAGIGDPILDERIKPGSLDAIVCALVL
                     CTVPDLSGALSRIESWLKPGGKLVLLEHVRSHGKITGAFQTAINPVWKVFAGGCSLNR
                     PTDLLVKNRKFKVQSEQQFRLTMPFYEATLERLSD"
BASE COUNT         1486 a         1485 c         1461 g         1472 t
ORIGIN      
        1 agccacccgg ccacccatga gttcgatatt tctgcggaca atggcaaggc ccaggcccgt
       61 tccgccaaag gagcgcgtcg tggatacatc gccctgttca aatggtttaa aaatattatc
      121 aatcttcttc ttcggaatcc cgatgccggt atcctgcact acaatttgca gtatcccttg
      181 atggaacaca gcgcggacgt caattttccc gacgcgagta aatttcaccg cattgccaat
      241 gatgttggtg aggatctgcc ccaggcgaag atcgtctgcc tttatgttct caggtacacc
      301 cggagcgatc gtcaacttca gttctactct tgcgtcctct tcgcgggatt ctatctggag
      361 gcgaaacatt ctgcatgtgt tttcgagcat ttcatgaagg ctgaatacgc gagcctccat
      421 ttgcattttg cccgcttcaa ttctggacaa atctagaagg ccgttgatcg tctcgagcag
      481 actcgttcct gccgagcgaa aagagctaac catacgtttc tgctcttcgt ccagtttcgt
      541 gccttccagg atctcagcaa ttcccagaat cgctgtgagg ggcgtgcgaa tttcatgact
      601 tacgttggcc agaaaaatgc tcttcgcctt atttgcattc tgtgcctgtg tggtagcatc
      661 ctggagcgct ctccttgcct ggcgatgctg cgttacatct tgcagggtat agatctttcc
      721 gatttcggtt ccgctggagt ccagaagaga atttctgcga acctggagcc atgacgagtc
      781 ggacacggag tattctgaag gagctcttgc cagttcatct gcgatcctct ggcctatttc
      841 cagactgtat ttatcgccta gcaggcgttt ggcggaggag ttgaaatcta caataactcc
      901 atgttgatcg cacaccagca cgccttcttc tatcatttca aatgtgcgct ctcgcgcaag
      961 ggggctaatt cgcagagtcc cgaaggaaaa aagggccgcg gtgagaagta aattgcctac
     1021 cgtgagggaa gccggggaaa tatcgagatt tggaatggga atgggcagca gtccgaaaac
     1081 tgtgaacagg gaggcgatcc agggaatggt cgcgctgagg atcatcagaa cgatttgagt
     1141 gcgaaagatc ccgtgaacgg aaggcagtct tcgcgcaaac aagaaacttc ctgcaagcag
     1201 gagcgaatag tagactgcta tgttggccca catgtagggg ccgtagtcgt agaccaggaa
     1261 ctgaacgcct cccatctctt tgacctgtgt tccatggcgg agcagtcccg tatccgtcca
     1321 cgcgatgatc tggttgatga caggaaaagc gagtaagaag aggggaggac gagtggtcct
     1381 ggtgtagtgc cacgcgaaga gaaaaaatcc aatgaccacc aggtcagagc cgatgaactc
     1441 caggttgtcc atcgccgtca taaaaggttg agtttttcca agtaattcgc cggcgtatcc
     1501 aaaggaccag atcgccgtgc caagcggcat cagaaaaaaa taaagagcac ctcgaactga
     1561 cctgcgccta aatgcgtacg ttccgttgat gactgagatt acagccgcga tagcgggcaa
     1621 gagggccagg gtttctgcat tcaatttgag ccgtgccgaa ttcgagcagc tgccaccaca
     1681 gcgaaaatgg gtgccagcgc cgctcccgca attggaacaa gcagaaacag aatgaatcct
     1741 atccctacac caagtatttc cggtcgatat ttctttgtga tggcgcgtcg ctccgcgact
     1801 gaaccagttt ctttttcaaa aatgaagtcg aacgcacttt ttcccaggat gtaagagttc
     1861 acgagaaaca tcaccggcgc ctggacagga ccaagaggaa gcgtaattac gaaaataaac
     1921 agtgcagcga tggatgcctg aacaccaagc cagccaccaa tcatcgcgtt tttgatttcc
     1981 ctgctcacgg ttgtttcaat ttcttttcca agcaaaactt tttcgacctg agcaaggatc
     2041 ggtccaatga aaggaagaac aagcatcgag gacaaaggac gataggcgat caccgtcaag
     2101 actagacccg caaagatgaa gagcagtctt aaagaagtgc gaacaaaatc aggcaagtcg
     2161 tgaaactgtt ccagtcgcag gaaaatgctc tctgatagtc caccggaaaa gaaaatcacg
     2221 caagcgccta acagtgcaac gacgagcaag cccgcaagag caggtaaaaa tatgtgcgaa
     2281 agcagattgt tttttctaag aaaacccagg cttgagaagt aggcccgaaa accggccgcc
     2341 aatcgagaaa gcatcagcgt acctggccgt gcaactcccc gtgctccaca ctggtgattc
     2401 tattttgtgt gtctacgtgg accacattcg ctttgtagtc gggagcgatt tctcccggct
     2461 ggaaaaaacc atatgccatt acaatgacaa ggtctccttt cgctgccatg cgagcggcgg
     2521 ctccgttgag gcagaccatg ccgctgcctc gctcgccttc aatgatgtag gtttccagtc
     2581 tcgatccgtt gttgatgttc acgacggaaa cctgttcata tgggtacaag cctgccgctt
     2641 caatgagatc caggtccagg gtcaggctgc ctggatattg tagctccgtc tgggtcacgg
     2701 tggcccggtg gatcttcgct ctgcacagtg atactaacat atcatttcct ctggtttcat
     2761 ctgcctatct gcgaccgctt gcttacatat tcagcgttga atggaatctc aactcttgca
     2821 atggtgcggt cctccagcgt gccgattcga aacaatgccg gatccagtcc ttcgctcttc
     2881 atcatgatca tgatgagtgc gattccgagg ccggcgccct cggtattgtc catattatcc
     2941 atataaaact cagcaatgtc gttatagctc atgctttttt tcatcttttc gcgcagcctg
     3001 agctcttctg cgggcgcaat tgccgtgttg ttttcgacct caatgcataa cccgtttggc
     3061 tgatgggaaa ggtagatttt cacatacacg cctttctctc tggacttgat tccgtagact
     3121 tcgttcatct catcagagaa tgcctggcga aactccagca ttcccctggc ataatcccgt
     3181 tcattggtaa tatcgagacc gtgttcgtca aaaaatagtc tcttctgatt agccttcgca
     3241 ccgttaatgg ccagttcctt tgtgattgtg tatactaatt cgagcagtga ctcacggtcg
     3301 taccgcgtga gtatcatctt caagatatga agaatatatt tttcgacttt ttcattgact
     3361 ttgctggact gcaaagtcag ttcttgactc tggttcagga ttagccgtat gttctgctca
     3421 agctcaaaaa agcttctgct cataaaaagg gcgcccgggg ccagaagtcc ttctatgtgg
     3481 cttgcgagca agtctgattc ctggagctaa tcatgttacc caccgctcag gcatccgaac
     3541 gcgccgctct gaagcgggcg attttcctgg acccggctgt atcgctggga aaagaccttg
     3601 ctatggaaat tcaccccctg gatgagctct acggctcaga tggtatgggt gcaggcctct
     3661 ctctgggggt ggccatcgca cagctggcaa cgttcgaaag tcacagggac cagttgtgga
     3721 actggattct gtcccatccg gaaacagaat tacgtctcat cgtcatttcg ccgcaagatc
     3781 atcccaagag ttactttcgt cccatcccgg aggaaattat ccacctggtt ttgccatcta
     3841 acgtttcgca gcaggtgctc actgacgtca tcgaaggcgc gtttcacgtt cttaaactcc
     3901 acaatgacaa agttcaacta caatcacgcc tggcactcag ctatcaggac attcgtcgcc
     3961 taacgcgtgt gggtcaggca cttgcaacag aaagaaattt tgaaactctc attggcctga
     4021 ttctgcaact ggcgcgagag attgttgctg cggatgcagg atccatctac gttgtggagc
     4081 ggcccagaca taacgaaaag ccaactcaca ttcgctttaa gaaatctgca atgaatctcg
     4141 cggcggatga atttcttctg ccaattgacc gcaattcaat cgccggttac gtggcgctca
     4201 ccggtcaacc tctcatgatc gaaaatgtgt acgcgctgag cggtcaggaa ggttacagct
     4261 tcaattacga gttcgatcgt cttcacaact attatacaaa gtccatgatg gtgattccaa
     4321 tgaagaacca tcgggatgaa gtgatcgggg tcattcagct catcaacaag aagagaagtt
     4381 ccgcgcgaaa gctcacgctc gaagatatga aagggcgcga agtggttccc ttcacggaca
     4441 attgcagaga gctcgtcagc gctctggcag ggcaagctgc tgtggccatc cagaatacaa
     4501 ttctcgttca agatatcaac aacctctttg aaggattcgt aaaagcttcc gtatttgcaa
     4561 ttgaacagag agatccaacc acttccggtc actcgttccg cgtcgcagag tttaccgttg
     4621 gacttgctca ggcagtagac agactttcct cgggtaagtt cgcagatctt aaattttctc
     4681 ccgatcaaat tcgcgaacta cgttatgcgt cattgttgca cgacttcggg aaagttggcg
     4741 tgcgtgagaa tgttcttgtg aaggccaaga agctgtatga gccggacatg gaactgattc
     4801 gctggagatt tcactacatc cggaaggctc tagagaaaga cgtactggaa aagaagttaa
     4861 agtactacaa agaaaacggt gcagtcggct ttgctcagta tgagaaatac gtggacctgg
     4921 aatttcgcga gaccatggaa gaggtaaatg agttcttccg catcatcaac gagtctaacg
     4981 agccttctgt gctggaagca ggcaactttg cgcggttgga agaaatttct cagcgcaagt
     5041 tcagagtaga cgatggctcg gacgttccct tcctcaagca aaatgaactg gtgagtctat
     5101 ctgttagaaa gggaaacctg gatcgccagg aaagactgga gattgagtcc catgtcagtc
     5161 acacctataa gtttctcgtg cagattccgt ggacctcaga gctcaggaga attccagaca
     5221 ttgcgcatgg ccatcatgag aagctggatg gttccggtta tccattaggc ctcaagtcgc
     5281 aggaaatttc tcctcagacc aggatgatga ccatttcaga tatttacgat gcgctcactg
     5341 caagtgacag gccttacaaa aagtccgttt cgcaggagcg tgccattgat attctcaagc
     5401 tggaagtgaa ggacggtcac gtggattcag atctgctcga catattcatt gaagcggaga
     5461 ttttcaaagt ccaatacaga tcctgatctt cagtcagaca ggcgttccag cgttgcttcg
     5521 tagaacggca tagtcaggcg aaactgttgt tcgctttgca ccttgaattt tctattcttc
     5581 acaagtagat ccgttggtct gttcagagaa catccacccg cgaatacctt ccacactgga
     5641 ttgatcgcag tttgaaaggc acccgtgatc ttgccatgcg atcgaacgtg ttcgagcaga
     5701 accagctttc cgccaggctt tagccaggac tcaatgcgcg aaagcgcgcc agacaaatct
     5761 ggaacagtgc aaagaaccag ggcgcataca atcgcatcga ggcttcctgg tttgattctt
     5821 tcatcgagga ttggatctcc gattcccgct tccacgagtt caatgtcttt cccgttgatt
     5881 cttgcccgtg ctcgacttgc cata
//
LOCUS       DAORFF010000200         5892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7264_length_5892_cov_3.903375, whole genome shotgun sequence.
ACCESSION   DAORFF010000200 DAORFF010000000
VERSION     DAORFF010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7264_length_5892_cov_3.903375"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..1629)
                     /locus_tag="PKK76_09365"
     CDS             complement(229..1629)
                     /locus_tag="PKK76_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004475442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNL68723.1"
                     /translation="MAKHRLVASPQLMISVSGIRGILPTGLSPSVIVDAVLGFGTVTG
                     KKIVIGMDSRNSGPLIRDLVCATLRAQGKEVLDAGLAPTPTVKAAVKLEHAAGGIMIS
                     ASHNPPEWNGLKLMGPEGFFFNKFNLSSMMTALESGKLTGADHKTLGSFHEIDAVSKH
                     IKTVLNLLNNVEQIRAKKYKVVVDAVGGAGRNALPELLTELGCEVIPLFCEAASGFDF
                     PRPPEPTPVALKKFSQLLKKESAAVGFALDPDADRIVPGSPGRGAINEEYSLPLSFLG
                     LPEIKDVPKSQPRGKTPFVVNLSTSTLLDQVAAPYGYPVIRSAVGEANVVEMMKKNKS
                     FFGGEGNGGIIHSRVPSFGRDSLLGAALILSAMARKNAATLDDVLNHLPELFMEKRKF
                     DVKAGTDVRELFSRIEGLFPGMRADRKDGLHLSDAEGWIHARSSNTEPVIRIIAEARN
                     KKHLEELLSRAARVVK"
     gene            1699..1781
                     /locus_tag="PKK76_09370"
     tRNA            1699..1781
                     /locus_tag="PKK76_09370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1733..1735,aa:Leu,seq:gag)
     gene            complement(2225..2776)
                     /locus_tag="PKK76_09375"
     CDS             complement(2225..2776)
                     /locus_tag="PKK76_09375"
                     /inference="COORDINATES: protein motif:HMM:NF020461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1851 domain-containing protein"
                     /protein_id="HNL68724.1"
                     /translation="MAHFPGFQKKFPKPDSCQPMSKSLEEYQGRLPGSLIEFLSEQGA
                     CSYAKGLIWTMDPFFMQDTLEEWLDEPENAVPFARTAFGELFLWQEGAAYYLNVQRGA
                     VTKVISSVEKFFDGFLLDENIQKKVFRKSLFAKALKEYGPISADEVYALTPALALGGA
                     ERVENLRKTKLREYVSFLAGLVE"
     gene            2879..3139
                     /locus_tag="PKK76_09380"
     CDS             2879..3139
                     /locus_tag="PKK76_09380"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNL68725.1"
                     /translation="MSLHPYARKREGENMIYQKNVPGWERAMRVGLGLLLVVAAAESI
                     YSGRPMLLSVGLLFSAIGIVATGFLGWCPACAMVGRKLKFKE"
     gene            3136..3672
                     /locus_tag="PKK76_09385"
     CDS             3136..3672
                     /locus_tag="PKK76_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNL68726.1"
                     /translation="MNTQYDKNIVEAARSGDMQALERLLLQCQPDIARFARNVCATPE
                     DVEDAVQETLWIVSRKIGTLRTVSAFASWAFRVVKHECYRLFRRVRRELSTDRVAEQS
                     YLDPGVDVPAMMRQETIQALANMPDLFRKVIVMRDIEGIPAVQVASELGLSIPAVKSR
                     LHRARSWMRLTLGHWMES"
     gene            complement(3706..4398)
                     /locus_tag="PKK76_09390"
     CDS             complement(3706..4398)
                     /locus_tag="PKK76_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNL68727.1"
                     /translation="MRTAVVFVLLSLNAWTACKEKPSFPSELTQYSEPVQGILPYFQG
                     TRMDPFWPHSKDRPADLRRMNNAIFRTEQDKPASALDHAKGKYSLVTFFYSSCVGLCP
                     LIAENMKNLSSRIEDQSDIQFISITIDPEIDDVKALQKFRAKHSIAQTNWILLTGDKK
                     ATESLAREQFAGEIKIKEGKAGLVDFVHTENVFLLDKEGYLRGVYRARGVGDFPRLLA
                     DLKRLRDGDRKN"
     gene            complement(4395..4988)
                     /locus_tag="PKK76_09395"
     CDS             complement(4395..4988)
                     /locus_tag="PKK76_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68728.1"
                     /translation="MIRSRFVFLFAAVLLLQDCSKQGPVIDAGSPGFEPKGIQLFYQG
                     KPFTGILRSKIPALDQVELAEYHAGLQHGITREQTLDGKLIAERPYYAGAKHGIHKTW
                     HENGQMKTFVEFKYGKYAGEAWSWYPSGKPFDYRKYNDQGELLLSRKWREDGQIYMNQ
                     VFQNGEAIGMPGSKLCDPAKNEKDQPASSNEKKENAP"
     gene            complement(4985..>5892)
                     /locus_tag="PKK76_09400"
     CDS             complement(4985..>5892)
                     /locus_tag="PKK76_09400"
                     /inference="COORDINATES: protein motif:HMM:NF025600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HNL68729.1"
                     /translation="DDNTLLLLSAAYVLTRSTNPCTNSAYSSGTAVTTSTLSLSSGCV
                     TGATSSMDSNLPTWIKNNFKCSVGYVQSTYYCFKSKNLLNARSPYYKASTGLNGGSGT
                     PNPNFIANQNLLYAIPMTPAKNNSTLSGTQNGLASIGIAVNGLAIFNNAANPPDTLST
                     EASTFDQQQGHPEMTGSYHHHSQLINGDGTGGTPASVNLTTQGSSALIGIALDGYAIY
                     GRWCGDATSGQNTQATGLDACHGHITTTPEFSTATYHYHYGGGASGTTCNGSGVGTGA
                     GNDATAGIPTIMGSYFYGVIGQVSN"
BASE COUNT         1336 a         1438 c         1540 g         1578 t
ORIGIN      
        1 agaggaagaa ctgacgttaa gcttttggcg gttctgccga tgcgtggagt atgcaggttg
       61 cagaacgatc gcaaacattt cggcctttcg aagcgactgt tgcctgtccc gattgcgggc
      121 atacgattga tctgtgggtg cagccaatgc acagggaaaa tcgcactctc tgtccagtct
      181 gcagcagggc cctgactgga atcattttgc gtttgattga aaaggaaact atttcacaac
      241 gcgcgctgcg cggctaagaa gttcttcgag atgcttcttg ttcctcgctt ccgcgatgat
      301 acggatcaca ggttccgtgt tgctggacct tgcatgaatc catccttccg cgtctgagag
      361 atggaggccg tctttgcggt ctgcccgcat tcctggaaac agtccttcga ttctggaaaa
      421 caactcccgg acatccgttc ctgcttttac gtcgaacttt ctcttctcca tgaaaagttc
      481 gggtagatgg ttcagaacat cgtctaacgt tgcggcattt ttgcgtgcca tcgcactgag
      541 aatcagagcc gcacccagaa gcgaatcgcg tccaaaggat ggcacgcgag agtgaatgat
      601 accgccgttc ccttctccgc caaagaacga tttattcttc ttcatcattt ctacaacgtt
      661 tgcttctcct acggcactgc gaatcacagg atatccgtaa ggtgcggcta cttgatcaag
      721 cagagtcgac gtggagagat tgactacgaa tggagtcttg ccgcgtggct gtgatttagg
      781 aacgtctttg atttccggga gacccagaaa tgacagaggc aaagagtatt cttcattgat
      841 ggcgccgcgt ccaggagaac ccggaacgat tcgatctgca tccggatcga gtgcgaagcc
      901 aacagcggct gactcttttt taagcaactg actgaatttc tttaaagcaa ccggcgtggg
      961 ttccggaggt cgcggaaaat caaaaccgga ggctgcttcg caaaaaagag gaatcacttc
     1021 gcaaccgagc tcggtgagaa gttcgggcaa tgcgtttctt cccgctccac cgactgcatc
     1081 aacaacgact ttgtatttct tcgctctgat ttgttctaca ttgtttagaa gatttaagac
     1141 cgtttttatg tgttttgaaa ccgcgtcgat ttcatggaag gatcccagcg ttttgtgatc
     1201 ggcacctgtc agtttgccgc tctcgagcgc ggtcatcata gaagaaagat taaatttgtt
     1261 gaagaagaaa ccttccgggc ccatgagttt caatccattc cattccggag gattgtgact
     1321 cgcggagatc atgataccgc ctgctgcatg ttcgagtttg acagcagcct tcactgtggg
     1381 agtgggagcg aggcctgcat ccagaacttc ctttccttgc gctcgcagag tagcgcatac
     1441 aagatcgcga atgagcggtc ctgaatttct cgagtccatc ccaatcacga ttttttttcc
     1501 tgtgactgtt ccgaaaccaa gcactgcatc tacgatgaca gaaggactca ggccagtcgg
     1561 gagtattccg cgaattcctg aaacagagat catgagttgg ggacttgcta cgagacgatg
     1621 tttggccatg ggggatgttg aaaaatggct ggaccgggcg cctggtcaaa acttttctag
     1681 tctctccaag ttttctgcgc ccggatggtg gaattggtag acacgcaagc ttgaggtgct
     1741 tgtgccgtaa tggtgtgggg gttcaagtcc cccttcgggc atttcgtaaa ccacgtttca
     1801 cccacgagca catttacacg cacactcacg ttcacgtcac gggactagaa ccacctgagt
     1861 tcgctgcgcg aacatcaggc acctcactcc ctatgggtcg ttcggtgggt tcgcgccccc
     1921 ttcgggcata ttccgcgcac gttttcggac accgcacaca tcactgctcg tcacgttcac
     1981 ggcacgggac tagaaccacc tgggttcgct gcgcgaacat caggcacgtc gctccctatg
     2041 aactatgaaa agacgaaact tcggcataca atctgagagt ctctaagaca cgcgcagtta
     2101 gctgcagcgt ccgggtgcag tccacagcgg cattcggagc gtccgagcgt cgcaacggcg
     2161 cgtcgcgagg atggactgca tccgggcgtt tgagcgttcg agctgcgcga agtcgtcaga
     2221 gacgtcactc aacgagtccg gccaggaaac ttacatattc acgcagcttt gtctttcgta
     2281 gattttccac tctttcagcg ccgcccaggg cgagagcagg cgtcagagcg tacacttcgt
     2341 ctgcggagat tggtccgtat tccttcagtg cttttgcgaa tagagatttt ctgaagactt
     2401 tcttttgtat gttctcatcc agcaggaatc catcgaagaa tttttccaca ctcgatatga
     2461 cttttgtaac tgcaccgcgc tgcacattca agtagtaagc ggccccttcc tgccatagaa
     2521 acaattcgcc aaatgcagtg cgtgcaaacg gaactgcatt ttccggttca tccagccatt
     2581 cttccagtgt gtcctgcatg aagaatggat ccatggtcca gatcaatcct ttcgcgtatg
     2641 aacaagcgcc ttgctcggat aagaattcga tcagcgatcc cgggagtctt ccctgatact
     2701 cttcaaggga cttactcatt ggctggcaag aatccggttt ggggaatttc ttctggaaac
     2761 cagggaagtg ggccataccc ggagaaaatg gtttgtaggt tgcttgtcaa atcgctcccg
     2821 acgaaccgct gaatttttcg catcggcaag aaaaagtctg aatccttttg caatccaggt
     2881 gtctcttcat ccatatgcgc gaaagcgcga aggagaaaac atgatttatc aaaagaacgt
     2941 tccgggctgg gaaagagcta tgcgcgtagg cctgggcttg ctgcttgttg ttgcggctgc
     3001 ggaaagtatt tactctggca ggccaatgct tctttcagtg gggctcctgt tctccgctat
     3061 tggaattgtg gcgacaggtt tcctgggctg gtgtcctgcc tgcgcgatgg ttgggagaaa
     3121 attgaagttc aaggaatgaa tacgcaatac gacaagaaca tcgtagaggc cgctcgaagc
     3181 ggagacatgc aggcactgga gcgacttttg ctacagtgtc agccggacat tgcaaggttt
     3241 gctagaaatg tttgcgccac tccagaagat gtggaggacg cagtccagga gactctctgg
     3301 attgtttccc gcaagattgg aacgcttcgc acagtctctg cgtttgcatc gtgggcgttt
     3361 cgcgttgtaa aacatgaatg ctatcgtctg tttagacgag tcaggcgtga actttcaaca
     3421 gacagagttg ccgagcagtc ctatctggac ccgggcgtag atgttcctgc gatgatgagg
     3481 caagagacga ttcaggcgct ggcaaacatg ccggatctat ttcgtaaggt gattgtgatg
     3541 cgcgatattg aaggaattcc tgcggttcag gttgcaagtg aactgggact ttccatcccg
     3601 gctgtgaaaa gccgccttca tcgcgcgagg agttggatgc gtcttaccct gggacattgg
     3661 atggaatctt gatgttttcc tttttgacag aaaggatgat agagttcaat tcttacgatc
     3721 accgtctcgc aaacgtttca aatcagcaag gagtcgagga aaatcaccca cgcctcgtgc
     3781 acgatacacg cctcgcagat atccttcctt atccaaaaga aatacgttct cagtgtgcac
     3841 aaaatcaacg aggccagctt tgccttcttt gatcttgatt tctcctgcga attgctctcg
     3901 tgccagactt tccgttgctt tcttgtctcc cgtaagaaga atccaattcg tctgcgctat
     3961 gctgtgtttt gcgcgaaatt tttgaagagc ttttacgtcg tctatttccg gatcaatggt
     4021 gatggagatg aactgaatgt cagactgatc ttcaattcgt gaagatagat tcttcatgtt
     4081 ctctgcgatc aaagggcaga gtccaacgca ggaagagtag aagaaagtaa cgagcgagta
     4141 tttgcccttc gcatgatcca gtgcgctcgc cggtttatcc tgttctgtgc gaaagatcgc
     4201 gttgttcatt cgtctcagat ccgccggccg atctttggag tgcggccaga aaggatccat
     4261 tctcgttcct tgaaagtaag gcagaattcc ttggacgggt tcggagtatt gtgtgagctc
     4321 agaaggaaaa gacggttttt ctttgcaggc agtccacgcg ttcaagctca agagtacaaa
     4381 aacgactgcg gttctcaagg tgcgttttcc tttttttcgt ttgaggatgc cggctgatct
     4441 ttttcattct ttgcaggatc gcacaatttg ctgcctggca ttccaatcgc ttcgccgttt
     4501 tgaaatacct gattcatgta gatctggccg tcttcgcgcc atttcctgga tagaagtagt
     4561 tctccctggt cattgtattt tctgtaatca aaaggtttgc cactcggata ccaggaccat
     4621 gcttctccag cgtacttccc atatttgaat tcaacaaacg ttttcatctg accgttttca
     4681 tgccaggttt tgtgaattcc gtgtttggct cccgcgtagt aagggcgttc cgcaatcaat
     4741 ttgccgtcta acgtttgttc gcgcgtgatg ccgtgttgaa gtcctgcgtg gtactcggcc
     4801 agctctacct ggtccaatgc tggaatcttc gagcgaagga ttcctgtgaa tggcttacct
     4861 tgatagaaga gttgaatgcc ctttggttca aaacctggtg aacctgcgtc tattactggt
     4921 ccttgtttgc tgcagtcttg taagagaaga actgccgcaa acaggaacac aaatctggat
     4981 cgaatcaatt gctgacttga ccaatcactc cgtagaagta ggaacccatg atggtcggaa
     5041 taccagccgt cgcatcgttg cctgcgcctg ttcctacacc ggagccgttg cacgtagttc
     5101 cagaagctcc tccaccgtag tgatagtggt aggttgcagt agagaattcc ggtgtagtag
     5161 tgatgtggcc gtggcatgca tccaggccag ttgcttgcgt gttctgaccg gacgttgcat
     5221 ctccgcacca tcttccgtag attgcatatc catccagggc gattccgatg agtgcactgg
     5281 aaccttgcgt tgttaagttc acgctcgccg gtgtgccgcc ggtgccgtct ccattgatta
     5341 gttgcgaatg gtgatggtag ctcccggtca tttctggatg accttgctgc tggtcgaaag
     5401 tggaggcctc agtcgaaagg gtgtcgggag ggtttgccgc attgttgaag atggcaagtc
     5461 cgttcactgc aatcccaatg gatgcaagac cattctgcgt acctgaaagt gtactgttgt
     5521 tcttggcagg cgtcattgga attgcataaa gtagattctg gttggcaata aagtttgggt
     5581 taggtgtgcc actcccgccg ttcaatccgg tggacgcctt gtaataggga cttcttgcat
     5641 tgagcaggtt ctttgatttg aaacagtagt atgtgctctg gacataaccc acggagcatt
     5701 tgaagttatt cttgatccac gtaggaagat tggagtccat cgagctcgtt gctccagtga
     5761 cgcaaccact actcagactc agcgttgagg tggtgactgc tgtcccggaa gaataagcag
     5821 agttcgtaca tggatttgta gagcgtgtca gcacatacgc cgcgctcaac agaaggagag
     5881 tgttatcgtc gt
//
LOCUS       DAORFF010000201         5888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7270_length_5888_cov_3.113492, whole genome shotgun sequence.
ACCESSION   DAORFF010000201 DAORFF010000000
VERSION     DAORFF010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7270_length_5888_cov_3.113492"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2131)
                     /locus_tag="PKK76_09405"
     CDS             complement(<1..2131)
                     /locus_tag="PKK76_09405"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL68730.1"
                     /translation="MRIFVLCIGLTFAGPLTAQHVVQNVKSLTQVESLDSTEQTPHRW
                     WVTDQPVDPVAITRQVLESKNENPGSAWKAIPVPSNVRDYYPHMTRKQMSVTYLKAVV
                     LPDEPAAATALMMGIINDRDRVYVNGTFIGATGIWDSPVATAYDKRRIYQIPAGVIRP
                     GELNVIVAQVQGLSVDLWGMDQYRTAIGPAMTIFSSEINESFRALLFLTCYTTVGLYF
                     FFMWARRRQDKQNLFFAVFCVLLIAYNLLRSQLRYDRGISFLELKRLEYIALYAVVPF
                     FFLFFRSYFHLPKTKVVKVFDGLMTLVCVVLLAGIGHTLVTNDTDGWWKFQRTIVQLG
                     CWPPLVVGSVGILVYRMFKKDKDALAMFISMILLVAAMVLDIASSMGKVNFPAIFQYV
                     FIAFILSIGTVLSNRFLRLHREIEDLNANLEQKIETRTRELNQTVAKARAIKEQQDGD
                     YFLTSLLIEPLSGVFSTGGPVHAESFVRQKKEFEFRKWKSDLGGDLAIVDRIGLRDKD
                     FTVFINADAMGKSMQGAGGAVVLGTTFQSLISRTKRGINASRHPEQWLRDAFFDLQNV
                     FVAFDGSMLVSAIFGLIDEASGTLYYINSEHPGAVLYRDKKAQFLTDAGLIRKFGIEG
                     IEENLHVNMLRLKPDDVVILGSDGRDDLHIGEDPQGGRIINDDESLFLRIVEEAQGNL
                     NEIVEGLTTRGQIIDDLSLIRLGYREDA"
     gene            complement(2193..3554)
                     /locus_tag="PKK76_09410"
     CDS             complement(2193..3554)
                     /locus_tag="PKK76_09410"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL68731.1"
                     /translation="MMDRLLNILFPERRELPYAVYIVRVKEQIRASLPYAGLLALVSW
                     LPYLYIDGQIHPEMQSALGALRLGLSLCGALALVARYTEFLGRFDMAAYYITATYLLQ
                     ATPIVTVLTHNDPQYVAGYQLALLVVSLFPLRFHLGLMQITGSLVTYHVFTYYYDREF
                     FIRSTYLWSNLAISFFVSMVIQYANERRRLKTEAHRRKLEETRFALKRRTEQIENDMS
                     IARLVQSNLMPHSFPDSHFIEIHGFYQSMELIGGDFYDVYVLDDHRQLLFIADASGHG
                     VSAALVAGMAKISFYHALDAGCSHPSDLLQFMNKEIKRFLRTEHFLTGFALLADSHTG
                     VVEYSNAGHRPPVYVRADGTMEHLDTPGTILGVLEDAGYETQSIPMRSGDFVALYTDG
                     IIESRNRKGEEFGPERLVGAVQKYRKEAISRVPFFIFSDLRVFSDGVVPGDDLTLLVA
                     RIK"
     gene            complement(3551..5047)
                     /locus_tag="PKK76_09415"
     CDS             complement(3551..5047)
                     /locus_tag="PKK76_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HNL68732.1"
                     /translation="MPSADLSFSFSNDSLDQIVCDVIIAGVFQNGETWESSGLMEELD
                     RLASCATVMKEEKFDASRDKTILIRTAQTTLRSKRLIIAGLGKKEKITTRNLSRALRP
                     AIDQALGFDAVGTIAICLSEMPPVVDSILSAVDMSHDVCYASRESKKPPKSCDQIILM
                     SRYGLSGTEGHLKAAVTIARARSSAKDLTNMPPNIKRTTTLVEKAKSLSSIPNVTVDI
                     VEDRAWIEKEMPCFFTVARGSLATDPPKFIRAHYTPGGAKKKIAIVGKSVIFDTGGYQ
                     VKTDDYMNTMKADMTGGAMALSLIEGFAGLGLDRIALSVYCAATPNMIDSEAMLPDSI
                     VATTCGKKVEIRHTDAEGRLTLIDAVAKACEDKPDLIITIATLTGSAARAVGEAIALM
                     GNETRYRERLALCAEKSGDACQTLDIWEDDYEDIKSKLDGADIINTSQNKNRGAQSAC
                     AFVMSGLTMEVPMLHLDIAGADMTKDEKATGIGVRSLMRFLIEENAAP"
     gene            complement(5079..5804)
                     /locus_tag="PKK76_09420"
     CDS             complement(5079..5804)
                     /locus_tag="PKK76_09420"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HNL68733.1"
                     /translation="MKRTALLLLFTGMITAAELPSPYDADGRIAIIERFLSERRGLRL
                     FVIDNFEGERTYHTRAASTQLADIQFIKRSPDSAPYRRELDLIASRAQPFEHEKSLFL
                     HFSFEDPGKQVFQVLPDHPVQIPGIPQRISLWVNGQSTHALVLLFQNSDGREVRVNAG
                     RLDHAGWTRVEMTAPDLLRSRGRRLERRYTTQFLGFVIESSPREEAGDASVMIDNLLV
                     LTDLSELMIQGEEIPDTWGRTKR"
BASE COUNT         1400 a         1444 c         1527 g         1517 t
ORIGIN      
        1 gcgcgtcttc tctgtatccc aggcgaatga ggctcaggtc atctatgatt tgaccgcgag
       61 tcgtcagtcc ttctacgatt tcgtttagat ttccctgtgc ttcttccaca attctgagaa
      121 aaagggattc atcgtcattg atgattctgc cgccctgcgg gtcttctcct atgtgaagat
      181 catcgcgtcc gtcacttccg agtatgacca catcgtccgg cttcaggcga agcatgttga
      241 cgtgtagatt ctcttcaatt ccttcgattc cgaatttgcg gatcaaaccc gcgtccgtca
      301 ggaattgcgc tttcttgtct cgataaagaa cagcgccggg atgttccgag ttgatgtagt
      361 acagagttcc agatgcttcg tctatgaggc caaagatggc ggacacaagc atgcttccgt
      421 caaacgctac gaacacgttt tgcagatcaa agaacgcatc gcgcagccac tgttccggat
      481 gtctgcttgc gttgattcct ctttttgtgc gagaaatgag ggattgaaac gtggttccca
      541 gtaccaccgc accgccggcg ccctgcatcg attttcccat cgcgtctgca ttgataaata
      601 cggtgaagtc tttgtcgcgc agaccgattc gatctacgat tgcaaggtct ccgcccagat
      661 ccgatttcca ttttctgaat tcgaattctt ttttctgacg aacaaaactc tccgcatgaa
      721 ccggcccgcc tgtggagaaa actccggaaa gcggttcaat gagaagggaa gtaaggaagt
      781 aatctccgtc ctgctgttct ttgattgccc gtgcctttgc caccgtttga ttcaattctc
      841 tggttcgcgt ttctatcttc tgttccagat tcgcatttaa gtcttctatt tcgcggtgaa
      901 ggcgcaggaa tcgattggag agtacggttc ctatgctcaa gatgaaagcg atgaagacgt
      961 actgaaaaat agcaggaaaa ttgactttcc ccatgctcga cgcaatgtcg agaaccattg
     1021 cggctacaag caggatcatg ctgatgaaca tggccaatgc gtccttatcc ttcttgaaca
     1081 tacgatagac aaggattccg acggagccaa ccaccagggg aggccagcat ccaagttgta
     1141 cgattgtcct ttgaaatttc caccacccat ccgtgtcatt cgtaacgagt gtatgaccaa
     1201 ttccagcgag taacaccaca cagacgaggg tcatcagacc atcgaagacc ttcacgactt
     1261 tagttttggg aagatgaaag taagagcgaa agaagagaaa gaaaaatggc acaactgcgt
     1321 agagagcaat gtattcgagt cttttcaatt cgagaaaact gatccctcga tcatatctca
     1381 actgactgcg aagcaaattg tacgcgatga gtaagacgca aaagaccgcg aaaaacaaat
     1441 tctgtttgtc ctgtctgcga cgggcccaca tgaaaaagaa ataaagtcca acggttgtgt
     1501 agcaggttag aaaaaggagc gcgcgaaaag attcgttgat ttcagatgaa aagatggtca
     1561 tggcaggacc gatggccgtc ctgtactgat ccattcccca gagatccaca gacagaccct
     1621 gcacctgagc tacaattacg ttgagctctc ctggtcgaat gactccagcc gggatctgat
     1681 agatgcgccg tttgtcgtaa gctgtggcca caggggaatc ccagattcct gtagcaccga
     1741 tgaacgttcc gttcacgtat actctgtctc tgtcgttgat gattcccatc atcagtgccg
     1801 tggctgctgc tggctcatcc ggtagcacaa ccgccttgag gtaggtcaca ctcatttgct
     1861 ttcgcgtcat atgcggatag tagtcgcgaa cgttagatgg aaccgggatt gccttccagg
     1921 cagagccggg gttttcgttc ttggattcaa gcacctgccg agtgatggca actggatcca
     1981 cgggctggtc tgtgacccac caccgatggg gcgtttgctc ggttgaatcc agggattcaa
     2041 cctgggtgag tgattttaca ttctggacta cgtgctgggc cgtgagtggt ccggcgaaag
     2101 tgagcccgat gcacagaacg aagatgcgca cggcggaata tggcccgtcg aggatgttcg
     2161 caaagcgttt ttctgatgcg ggaaagtaaa aactatttaa ttctcgcgac aagcaaggtc
     2221 agatcgtctc ccggaaccac accatcggag aacactctga gatccgagaa gatgaaaaaa
     2281 gggacgcgag agatcgcttc cttcctgtac ttctggactg ctccgacgag gcgttcgggc
     2341 ccgaactcct ctccctttct gttgcgactc tctatgatgc cgtctgtata aagcgcaaca
     2401 aaatcgccgg atcgcatggg tatggattgc gtttcgtacc ccgcgtcttc tagaactccg
     2461 aggattgtgc caggcgtatc gagatgttcc atggttccat ccgcacgcac gtacactggt
     2521 ggccgatgac cggcgttgct gtattctacg acgccggtgt gcgaatccgc aagcagtgcg
     2581 aaaccagtta gaaagtgttc ggtgcgaaga aatcgtttga tctctttgtt catgaattgc
     2641 aggagatcgc tgggatggct gcagcctgcg tccagagcat gatagaaaga aatctttgcc
     2701 attccagcca cgagtgctgc ggatactcca tgaccggatg cgtctgcgat gaacaacagc
     2761 tgtctatgat catcgagtac gtaaacatcg tagaaatctc cgccgatgag ttccatgctc
     2821 tggtagaagc cgtgaatctc aatgaagtgt gaatcaggaa aagaatgggg catgaggttg
     2881 ctttgcacga ggcgcgcgat gctcatgtcg ttttcaattt gttctgtccg tcttttgaga
     2941 gcaaacctgg tctcttccaa ttttctgcga tgtgcttcag tcttcaggcg acgcctttcg
     3001 tttgcgtatt gaatcaccat cgacacaaag aaagaaatgg caaggttgct ccacagatag
     3061 gtggatcgaa tgaaaaactc acgatcataa tagtatgtaa atacatggta agtgaccaga
     3121 gatcctgtga tctgcatcag accaaggtga aatcgaagtg gaaagaggct cacaacgagc
     3181 agagcaagct ggtagcccgc tacatattga ggatcgttgt gtgtgagaac cgttacgatg
     3241 ggagtagcct gcagcaaata ggtggcggta atgtagtaag ctgccatgtc aaagcggccg
     3301 aggaattccg tataacgtgc aaccagagcc agcgccccgc acaaactcaa tccgaggcgc
     3361 aacgccccca gagcgctttg catttccgga tggatctgtc cgtctatata caggtatgga
     3421 agccagctta caagagcaag cagccccgcg tagggtagag aagcgcggat ttgttcttta
     3481 acgcgcacga tataaactgc atagggcagt tcgcgtcttt cagggaacag tatgttgaga
     3541 agccgatcca tcatggagcg gcgttttcct cgatgagaaa acgcatgagg gatcgcactc
     3601 cgattccggt tgctttctca tcttttgtca tatccgcacc tgcgatgtcg aggtgaagca
     3661 tgggcacttc catggtcagg ccgctcatca caaatgcaca ggcagattga gctccgcgat
     3721 tcttgttttg acttgtattg atgatgtctg cgccatcgag tttgcttttg atgtcttcgt
     3781 agtcatcttc ccagatgtct agagtctggc acgcgtctcc gcttttctcc gcacagagtg
     3841 ccaggcgctc ccgatagcgt gtttcatttc ccataagggc gattgcttcc ccgacagcgc
     3901 gcgcagcaga tcccgtgaga gtggcaatgg tgatgatcaa gtccggcttg tcttcgcagg
     3961 ctttcgcaac agcatcaatg agagtgagtc gtccttctgc gtctgtgtgg cgaatttcaa
     4021 ctttcttgcc gcaggtagtg gcaacaatag aatccggcag cattgcttcc gaatcaatca
     4081 tgtttggagt ggcagcgcag tagacggaca acgctattcg gtctagcccc aggccagcaa
     4141 atccttctat aagagaaagt gccatggcgc cgcctgtcat gtctgccttc atcgtattca
     4201 tgtagtcgtc tgttttgacc tggtatccgc ctgtgtcgaa gatgacgctt tttcccacga
     4261 tggcgatttt tttcttcgcg cctccgggag tgtagtgggc gcgaatgaac ttcggaggat
     4321 ccgttgccag agaacctctg gccactgtaa agaagcaggg catttctttc tcaatccagg
     4381 cgcgatcttc tactatgtct acggtgacgt ttggaataga ggagagtgat tttgctttct
     4441 ctacaagagt ggtggtgcgc tttatattgg gcggcatgtt cgtgagatct tttgctgaac
     4501 tgcgtgctcg cgcaatggtc actgctgctt tgagatgtcc ttccgtgcca gaaagaccat
     4561 agcgagacat gagtatgatt tgatcgcacg actttggtgg cttcttagat tcgcgcgacg
     4621 cataacagac gtcatgcgac atatcgactg cggagagaat cgaatcgact acaggaggca
     4681 tctcactgag acaaatggcg atggttccca ctgcgtcaaa tcccagggcc tgatcaatgg
     4741 ccggcctgag tgcgcgagat agattgcgtg ttgtgatttt ctcttttttg cccagacctg
     4801 cgatgatcag tctttttgag cgaagagttg tttgcgctgt gcggatcagg atggtcttgt
     4861 ccctggatgc atcgaacttt tcttccttca ttacagtcgc gcaggaagca agtctgtcga
     4921 gttcctccat gaggcccgaa gattcccagg tctctccgtt ttgaaaaaca cctgcaatga
     4981 ttacgtcgca aacgatttga tccagagaat cattggaaaa agagaaggag aggtcagcgg
     5041 aaggcatgaa atcattgtcc tttccgccgt aaatgtgtct acctttttgt ccgaccccag
     5101 gtgtccggga tttcttctcc ctggatcata agctcgctca agtctgttag aacaagaagg
     5161 ttgtcgatca tgacgcttgc gtcgcctgct tcttcgcgtg gactcgattc aatcacaaag
     5221 cccaggaact gagtggtgta tctgcgttcg agtcttctac ctctagatct caagagatcg
     5281 ggtgctgtca tctccactct ggtccatccg gcgtgatcca gtctgccagc gttcactctc
     5341 acttcgcgtc catctgaatt ctgaaacaac aagaccagag catgggtgct ttgtccgttc
     5401 acccatagtg atattctttg cgggatccct ggaatctgca ccggatgatc cggcagtacc
     5461 tgaaacacct gtttgcctgg atcttcgaat gaaaagtgaa gaaacaggga cttctcatgt
     5521 tcgaaaggtt gagcgcggga ggcaatgaga tccagctcac ggcgataagg ggcggagtcc
     5581 ggcgatcttt tgatgaattg aatgtcagcg agttgagtgg atgcggctct ggtatgatac
     5641 gtgcgttcgc cttcgaaatt gtcgatcacg aagagacgca gtcccctcct ttcggataga
     5701 aatcgttcta tgatagcgat ccgaccgtcc gcatcgtatg gggaaggaag ctcagccgcc
     5761 gtgatcattc ctgtaaagag caaaagaaga gcagttctct tcatatataa cagtgacgga
     5821 agattccgcg ttttacatga gcctaggctt ctgagccacg ccgtttagga ggacgctttt
     5881 tctttgct
//
LOCUS       DAORFF010000202         5836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7377_length_5836_cov_3.514098, whole genome shotgun sequence.
ACCESSION   DAORFF010000202 DAORFF010000000
VERSION     DAORFF010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7377_length_5836_cov_3.514098"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..639
                     /locus_tag="PKK76_09425"
     CDS             118..639
                     /locus_tag="PKK76_09425"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNL68734.1"
                     /translation="MLKKILKWVGIVVGVFVLVGLLLPRAAKLERSIVIQAPADKVFV
                     YVNSLEKFQEYSPWRDMEPEAAVTMGEKKEGVGASFSWKGKKTGEGSMTITESVKNER
                     VVTALDFKDQGKAGATFLLKPQGAETQVTWTFEGDAGYNLISRYFYLFIDKLLGSDYE
                     KGLAKLKKVVEKK"
     gene            complement(718..2022)
                     /locus_tag="PKK76_09430"
     CDS             complement(718..2022)
                     /locus_tag="PKK76_09430"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNL68735.1"
                     /translation="MKLILAALIAFSACVPSRAEPISSGPMAGNQSPRTVTLWLQTNG
                     VESGEIKYSNVKLKGVPRSFKFTTSSEDDYALHAELSDLEPASTYTYEIIFKSKTYGP
                     FEFRTLRNGKSEEEFRVLTGSCAFINDSRFESGKPYGAHYEIFETMAKANPDVMLWLG
                     DNSYLRDVDFLNPWSMNQRFRKDRSLPQMQALLHKGHHYAIWDDHDYGPNDSTSSFIF
                     KDHSLKFFKRYWANPTYGLNETPGIFTSFRKGDAEFFLLDDRFNRDADRSPNRPEKQM
                     FGPAQMRWLKNALVNSNASFKIIAGGSQMFNDANKFEGWQNFPQERKDFLDFVAEARI
                     TGLFFLTGDRHHTELAKIERPGTYPIYELTCSPLTSGIHPVSEEEKKNGKHLPGTLIE
                     ERNFCQLDFGGAGSDRRITVSSFDPTGKMLWEKKIQLSELGK"
     gene            complement(2019..2387)
                     /locus_tag="PKK76_09435"
     CDS             complement(2019..2387)
                     /locus_tag="PKK76_09435"
                     /inference="COORDINATES: protein motif:HMM:NF025384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HNL68736.1"
                     /translation="MKRSPALVFLLGLCTLGLYLLFWYNRIYNELKELGGKTPTGNSY
                     GFDLLLTIVTCGVWGIYVDYRISLTLDEILVARSMPAQNSAPLVLVLDIFFYVTGGLT
                     YLATSAIHQDIINRVYEQKA"
     gene            complement(2387..2806)
                     /locus_tag="PKK76_09440"
     CDS             complement(2387..2806)
                     /locus_tag="PKK76_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNL68737.1"
                     /translation="MKRIQFNEFEVGAKLPELKVGPVQHMDLVRYSGASGDFNPIHTD
                     PTFAKSVGLDGTIAHGMYVMAQVGRMCTNWVHPAQIQFYGIKFKGMTKPGEMIVCTGE
                     VKKKKEEDGKKLMTVSVSAANEAGEVKAAGDLVVVCD"
     gene            complement(2817..3263)
                     /locus_tag="PKK76_09445"
     CDS             complement(2817..3263)
                     /locus_tag="PKK76_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HNL68738.1"
                     /translation="MALSKDIVGKKLDSYQFTVERGKVKEFCRAIGETNPIYTDPAEA
                     KKAGFQDTPIPPTMQTTFQFWGYPKIWDDMKGMGIDISRLLHMKEEYTYLKPVYPGTT
                     IHAAGEVIDVKTGKMDMVTFKTTYANEKGEPCILAEMAIVIRPEGT"
     gene            3361..4008
                     /locus_tag="PKK76_09450"
     CDS             3361..4008
                     /locus_tag="PKK76_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNL68739.1"
                     /translation="MMVQRTTKRKILGMLAVLGFFSLVRDHWAEPEERLLQRSFLTGY
                     IQRTHPQIHSVDRDRLVLAILDQAGGLALSDQKIDGAPVDRVLFLTAVIETESTFKSD
                     AISSMDARGYMQLLPSTAKWMGDKFGLGVPATQDLFRPEVNLPRGVIFLNHLNEQLRD
                     IRLVCLAYNAGLGNVQRGYWDERYWQKIYINYRRLSSEAEDNRWMSREVTGVRDI"
     gene            complement(4019..4900)
                     /locus_tag="PKK76_09455"
     CDS             complement(4019..4900)
                     /locus_tag="PKK76_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68740.1"
                     /translation="MRTRLFLLFFSSICAVTAVYANMRQRWQVDRYPSYFPQSVPGTT
                     VDSEDLNFICDLAYEGDADWSEMQKRGCNVRALYAITTKIERGVALEFILPSEDPVTV
                     FINGEKVKVDSREIVMTDTTRRAYRLSEVCHFCGSKPSRLYSASFDGKLRAGRNEIEV
                     QYRQPYSFYETAYGYFKTSEWLNSFEYELWPLRSWEVSPGFKMNIRVQLQRPGWLKRI
                     TTDSKLECSGENLQIPTHFKLPPQSTSENNFESWIKQNTRTDVKLNRRNEADQAIFTT
                     TMTENFPDRLSCIVQSD"
     gene            4972..5448
                     /gene="nudB"
                     /locus_tag="PKK76_09460"
     CDS             4972..5448
                     /gene="nudB"
                     /locus_tag="PKK76_09460"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: protein motif:HMM:NF006961.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HNL68741.1"
                     /translation="MGWVIPLAQPTPLTSPMHYKVPESALVVVYSKDQFLLLERSDRP
                     GYWQSVTGSRDVHETIEQTAARELKEETGLTMADGVLISTGRTHTYPIMDEWRHRFAP
                     DVSENRETVFYFSMPQVCNVTLSEHIRYEWVSGEEAVGRVFSRTNREEIQRILMTV"
     gene            5499..>5836
                     /locus_tag="PKK76_09465"
     CDS             5499..>5836
                     /locus_tag="PKK76_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL68742.1"
                     /translation="MAKKKQAIPKKAARKTYELQRVNFPSGSGGCEADLYIPKGRGNH
                     PVVVMAHGFGAERSFRLPAFADRFAEAGIAVLLFDYRSFGDSPGEPRNLINPRMHNED
                     WKAALQFVRR"
BASE COUNT         1398 a         1352 c         1499 g         1587 t
ORIGIN      
        1 attttgcgcg aggagcactt ctgcttcttc ctgggtcagg ccgaggaatg caccgtctgt
       61 catgggaaca tctcactttc gcttgcagaa agttcaagcg cattgcaaac gtggttcatg
      121 cttaagaaga ttttgaaatg ggtcgggatt gtagtcggtg tgtttgttct tgttggattg
      181 ctcttgccta gagctgcgaa actggaacgt tccattgtca ttcaggctcc ggcggacaaa
      241 gtgttcgtct atgtgaatag tctggaaaaa tttcaggaat actctccgtg gagggatatg
      301 gagccagagg ctgccgtcac gatgggagag aagaaagaag gcgtgggcgc ttcctttagc
      361 tggaaaggaa agaagacagg cgaaggaagc atgaccatca ctgaaagcgt gaagaatgag
      421 cgagtggtaa ctgcgctcga tttcaaggat caggggaagg ctggcgcgac gttcctgcta
      481 aaaccgcagg gcgctgaaac tcaggtcacc tggacttttg aaggggacgc tggctacaat
      541 ttgatatcca gatatttcta tttgttcata gacaagctac ttggatcaga ctatgagaag
      601 gggcttgcaa aactcaagaa agttgtagag aagaagtaag ttgaaccagg catcacgata
      661 gttcaggaac tacgattttt tcgcatgaat cgccttcggg cgattctacg cgaagagtta
      721 tttgcccagt tctgaaagct ggatcttttt ttcccacagc atcttgcctg ttggatcaaa
      781 cgatgatacc gttatgcggc gatcagatcc tgctcccccg aaatccaact ggcagaaatt
      841 tctctcttct atgagagtgc cgggtaaatg ctttccattt ttcttttctt cttcactgac
      901 tgggtgaatc ccgctggtca gaggggaaca cgttagctca tagatcggat aggtgccagg
      961 acgttcaatc ttcgccagtt cagtgtgatg tctgtctccc gtcaggaaaa aaaggcccgt
     1021 tatgcgcgct tccgcgacga aatccaggaa gtcctttctt tcttgcggga aattttgcca
     1081 gccttcgaat ttgtttgcgt cgttgaacat ctggcttccg ccagcgatga tcttgaaaga
     1141 tgcatttgag ttaaccagtg catttttcag ccatcgcatt tgagcaggac caaacatttg
     1201 cttttccggt cgattcggag atcgatccgc atcgcggttg aatcggtcat cgagcaaaaa
     1261 gaattctgcg tcgcctttgc gaaaagaggt gaatatgcct ggggtttcgt tcaatccata
     1321 ggttgggttg gcccagtaac gcttgaaaaa cttaaggctg tggtctttga aaatgaaact
     1381 cgatgtggag tcattgggac catagtcatg atcgtcccag atggcgtaat gatgcccctt
     1441 gtgcagcagt gcttgcattt gagggagaga tctatctttg cgaaagcgct gattcatact
     1501 ccagggattc aagaaatcca catcgcgaag gtaagagttg tctccgagcc agagcatgac
     1561 gtcaggattt gcttttgcca tggtttcgaa tatttcgtag tgggccccgt agggttttcc
     1621 tgattcaaag cgagaatcgt ttatgaaggc gcaggagcca gtcaggactc tgaactcctc
     1681 ttcgcttttg ccattgcgta gagtgcgaaa ttcgaatggg ccgtatgttt ttgatttgaa
     1741 aatgatttcg taagtgtaag tggatgctgg ctctagatca gaaagctctg cgtgcaacgc
     1801 atagtcgtcc tcagaagagg ttgtgaattt aaaagaccgc gggacccctt tgagttttac
     1861 attggagtat ttgatttccc ctgattctac tccattcgtc tgcagccaga gtgtgactgt
     1921 gcgaggagac tgattgcccg ccatgggtcc agaggagatc ggctctgccc gcgaaggaac
     1981 gcaggcagag aacgcgatga gggcagcaag aattagtttc aagctttctg ttcgtaaacc
     2041 cggttgatga tgtcttgatg aatggcagag gttgccagat acgtcaaacc acccgttaca
     2101 tagaaaaaga tgtccaggac cagaaccaga ggagctgaat tctgtgcagg catgctgcgc
     2161 gccacgagga tttcatcgag agttaaagat attctatagt ctacatagat tccccagaca
     2221 ccgcatgtaa caatggtcag gagaagatcg aagccgtacg aatttccagt gggtgtcttt
     2281 ccgcccagtt ctttgagttc gttgtagatg cgattgtacc agaatagaag atacaatcca
     2341 agagtacaaa gaccaagtaa gaatacaagt gcaggagagc gtttcatcag tcgcacacca
     2401 ctacaagatc gcccgctgct ttcacttcgc cggcttcatt ggccgcggaa acagaaacag
     2461 tcatcagttt cttgccatct tcttctttct tctttttgac ttcgcctgtg cagacgatca
     2521 tttcgcctgg ctttgtcatt cctttgaact tgattccata gaattgaatt tgagccgggt
     2581 gcacccagtt tgtgcacata cgccccactt gcgccatcac atacataccg tgcgcgatgg
     2641 ttccatccag tcctacgctt ttagcgaaag taggatctgt atgaatcgga ttgaagtcac
     2701 cggatgcgcc ggaatagcgc accagatcca tgtgctgcac cggcccgact ttcagctccg
     2761 gcagttttgc gccgacttcg aattcgttga attgaatgcg tttcatgttt tgctcctcaa
     2821 gtcccttccg gacgaatcac gattgccatt tctgccagaa tgcagggttc gcctttttca
     2881 ttcgcatacg ttgttttgaa tgtaaccata tccatctttc cagttttcac atcgatgact
     2941 tcccccgctg cgtggatggt cgttccgggg taaactggtt tgaggtaagt atactcttct
     3001 ttcatatgta gaagacggct gatgtcgatt cccatgcctt tcatgtcgtc ccagatcttg
     3061 gggtatcccc agaactgaaa tgtagtctgc atggtgggag gaataggagt gtcctgaaag
     3121 cccgcttttt tggcctccgc cgggtcagtg tagatcgggt tggtttctcc gattgcgcgg
     3181 cagaattctt ttacctttcc gcgttccacc gtaaactgat acgaatcgag ctttttgccc
     3241 acaatgtctt tgctgagtgc catatcgctt tgtgggatcg caccttttcg ggtcaagcta
     3301 catgcattct ggaccccaaa aaggacttta cctggtgcaa cgcaaaaaaa tcatggcttc
     3361 atgatggtgc aacgcacaac aaaacgcaag atcctgggga tgctggcagt gctcggcttc
     3421 ttttccctgg tccgcgacca ctgggcggaa ccggaagaga ggctgctgca gcgatctttt
     3481 ctgactgggt acatccagag aacccatcct caaattcatt ctgtggatcg tgatcggttg
     3541 gtcctggcca ttctggatca ggccggcggt ctggctcttt cggatcaaaa gatcgatggg
     3601 gcccctgtgg atcgggttct gtttttgact gcggtcatcg aaacagaatc cactttcaaa
     3661 tccgacgcaa tctcctctat ggacgcgcgc ggatacatgc aactgctccc gtctactgca
     3721 aagtggatgg gcgataagtt cgggctggga gtacctgcta cgcaggattt gtttcgtcca
     3781 gaagtgaacc ttcctcgtgg cgtgatcttc ctcaatcatc tcaatgagca attgcgcgac
     3841 attcgcctgg tctgtctcgc atacaatgcc ggtctgggca atgtgcaacg cggatactgg
     3901 gacgaaagat actggcaaaa gatatacatc aactatcgca ggctttcttc ggaagcagaa
     3961 gacaatcgtt ggatgagccg ggaagttacg ggagtgcgcg atatctaatc gcgcattctc
     4021 agtccgattg cacaatgcac gagaggcgat caggaaaatt ttcagtcatc gtagtcgtga
     4081 aaatggcctg atctgcttca tttcttcgat tcaatttaac atcggttcgc gtgttttgtt
     4141 tgatccatga ttcgaaatta ttttcggatg tgctttgagg cggaagcttg aagtgagtcg
     4201 ggatttgtaa gttctcaccg ctgcattcta gtttgctgtc tgttgtgatt cttttcaacc
     4261 atccgggtct ctgcaattgc actcggatgt tcattttgaa cccgggggac acttcccagc
     4321 tgcgcagagg ccatagttcg tattcaaatg aattgagcca ttcacttgtc ttgaagtaac
     4381 cgtatgccgt ttcgtagaaa ctgtatggtt gtctgtattg tacttcaatt tcatttcttc
     4441 ctgctcgcaa tttcccgtca aaggacgctg agtatagacg tgagggtttt gatccgcaga
     4501 aatgacagac ttcactcagg cgatacgcgc ggcgtgtggt gtctgtcatg acaatctctc
     4561 gtgaatccac tttcactttt tctccgttga tgaacacggt cacagggtct tccgacggta
     4621 gaatgaattc cagcgccaca cctcgctcta tctttgttgt gattgcatac aaagcgcgca
     4681 cgttgcatcc tctcttttgc atttcgctcc aatctgcatc accttcgtaa gcaaggtcac
     4741 agatgaaatt gagatcttct gaatctacag tggttcccgg cacactctga ggaaaatacg
     4801 aggggtaacg atccacctgc cagcgttggc gcatgttcgc atacactgca gtgacagcgc
     4861 aaatggagga gaaaaagaga agaaagagcc ttgtgcgcat gcctgaaaat aattgaagtc
     4921 tttagcccgg aaagtgcttt tcagcctgct cgggtccgat atattgacag tgtgggctgg
     4981 gtgatccccc tcgctcagcc cacacctctt acatctccca tgcactacaa agtcccagaa
     5041 tccgctctgg tcgtggtgta ttcaaaagat cagtttcttc tattggaaag atcggacagg
     5101 cctggctact ggcaatcggt tacggggtct cgcgatgtgc atgaaacaat cgaacagact
     5161 gctgctcgtg aattgaaaga agaaacgggc ctcacaatgg cagatggtgt tctaatttct
     5221 acgggacgca ctcatacata tccaatcatg gatgaatggc gccatcggtt tgcgccagat
     5281 gtatctgaaa acagggagac agtgttttat ttttccatgc ctcaagtttg caacgtcact
     5341 ctgtcggaac acattcgata cgaatgggtt tcaggggaag aggccgtcgg tagagtcttc
     5401 tcaagaacaa atcgggaaga gatccagcgg attctcatga ctgtttgatt ggtctagaaa
     5461 tgaagtgcgc tccaaaacga ctgcggtgcg attgacacgt ggccaaaaag aaacaggcga
     5521 ttcctaagaa agccgcgcga aaaacgtacg agttgcagcg tgtaaatttt ccgtctggtt
     5581 ccggtggttg cgaagcggat ctttatatcc cgaagggtcg tgggaatcat ccggtggttg
     5641 tgatggctca tggttttgga gctgaaagaa gtttcagact tcctgcgttc gcggatcgct
     5701 tcgcggaagc tggaatcgca gtgctcctgt ttgactatcg aagcttcgga gatagtcccg
     5761 gtgagccgcg aaatctgatc aatccgcgca tgcacaatga agactggaaa gcggcacttc
     5821 aatttgtccg cagaat
//
LOCUS       DAORFF010000203         5820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7405_length_5820_cov_3.733738, whole genome shotgun sequence.
ACCESSION   DAORFF010000203 DAORFF010000000
VERSION     DAORFF010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7405_length_5820_cov_3.733738"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PKK76_09470"
     CDS             <1..970
                     /locus_tag="PKK76_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNL68743.1"
                     /translation="TAQTDFPLEMLRASRSNFFPRGIGDMAWKRTVKIGVLGGHEGVY
                     NPRATVILGVLTFVIVYFFPSSHAIESMYGLAVILTMNIGIFAYLLRQIRAHKINGMT
                     VIGMLVMTVMLFNIVYNKFFDGAWFVVVLLFAYLGIFVVSEAIYAIWLEKLNVVPLEL
                     GLWYPIFNDRKVDYKNIVLVSKFHPGVIHFLKNYVKSGHIPLVVHFRTDFDEDVPAQV
                     PAWYKNIDVPESTDTITAITRYVKRMKPQRVHLIPALVRGIDPISRYYFGNSMERLKY
                     AVSQFADLQVEYNKERIHIRVGDIVKRLFPRLGDRFFSDQKPTAEG"
     gene            complement(1021..2427)
                     /locus_tag="PKK76_09475"
     CDS             complement(1021..2427)
                     /locus_tag="PKK76_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA domain protein"
                     /protein_id="HNL68744.1"
                     /translation="MKKILYFVVIPLFVILALVSASAWYALKVVLNENFLVQQIESNL
                     NVRAEVRKLNVSLFSAMSSVELEGVSLGARDSFANQGTPLSERPPMKGSVVTIGKIDL
                     KFSLLPLLRRKFELNRFMLSSPVVSMTLFANGGNSLKPLFQKPLIVGRKPNPALAEKP
                     AETAAKEEEKPFSAKDLPISADLREIGLKDATVNLLMQKTGQLIRISGLALVIDSIDI
                     DPADLVKHNNAHLDFDANVNVISAKQTDAASFLIRSSGNVTPFDSKTGEVSTGVEYAL
                     VVKKDSFITGMAIFDQLAGELPVLSQVGFSWKKLSEKAVLLRDVNVRVRYSHALVTFL
                     DDTVFPTANYDLLLKKGSTINCATNEHNFNASVRATEAESKEILARFDRGIEKKLKGQ
                     SVEDVRNRVLGKLIQNGQVDLPFASLGNFANPSVKLLAELPSLTEVLTGSVKDILKNK
                     LDQKIPGVGKELLNKLPF"
     gene            complement(2486..3940)
                     /locus_tag="PKK76_09480"
     CDS             complement(2486..3940)
                     /locus_tag="PKK76_09480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011671577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNL68745.1"
                     /translation="MIAAAGKGTRAYPRTSYVPKPLFTFENQTLLEKNVYIQLKVLKV
                     PELYIIVGHLKEQVLSEVERIRRHYPHAKIHTAEWTGKGLASDIAALGDRIDGDFSVI
                     LGDEFYMDMNHQALAKLWSQKKKAEAMIAVLKSDLLSEIRKNYSVELEGTRVMKLVEK
                     PDSPPNDLLGLGTYIFSPEYFKAFRETPPSNKSGVVELTDVIDLMSHKYEVHAGLVKG
                     RYYNINSLADYYSATCRIRSEKFHKFRTTLVVPAFNNERTLPDVLQDFRGRVNEIIVA
                     DMGSSDRTVEIARKAGAKVIQATESAGNPHGAQIIYRAFREASGDILVLATADGNFRA
                     NDLPKLLEYMKDSDMVVGTRTTRQLMEQGTNLGPLYRWLNVALGKLLEILWWDLEPRF
                     TDIGCIYRAIWKDSFLKIAEELRATDRTYLAEMMIGILRFHMRCIEIPVSLYRRYGSV
                     RTETTSEQWQYFFSVLRLIFGRRFTLLGKLFRVS"
     gene            4059..5471
                     /locus_tag="PKK76_09485"
     CDS             4059..5471
                     /locus_tag="PKK76_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68746.1"
                     /translation="MKATTFGELKSQGYRYRSVRTEMAENLAAKIRSKQQIFPEIHGY
                     EDTVIPQLTHAILSGHNIAILGEKGQAKSRIMRSLTTLLDDEFPVLTGTEIPESPFHP
                     ITALGRQHVAAGDSAPIRWLSRDERYGERLAPGSRIADIIGDLDPSRIIAGESLGSES
                     ALHYGLLPRMNHGIFAINELPDLDYLVQVALFNVLEEADIQIRGLPIRIPLDVFVCFT
                     ANPADYSRSGKIITQLKDRIGAEIRTHYPKARSVGLAITRQEIQNLIVDPRIIVPEFM
                     ENIIEEITIQARMSPVVNQKSGVSARLSIANYETATASARRRALLLGENPGHVRLTDL
                     GNLFASSSGKIELDPYRDEAVTEFQVIQKIIDEAIREVFLEYFPLKKYEPTMDQVAKQ
                     IFEAKGLEISDTMNVESYRDILKKVPALFDFMRDRGWDSDSQTLACGVEFILEGLAVN
                     QKLSRRKLGEIVSFKSVDIY"
     gene            5526..>5820
                     /locus_tag="PKK76_09490"
     CDS             5526..>5820
                     /locus_tag="PKK76_09490"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNL68747.1"
                     /translation="MKQHTRGLGLYFIASGTAAAVNFGSRFVYDSFFTFGTSVVLAYC
                     TGMFVNFSLSKLFVFDARTSGRTWREFTKFIAVASLGLVVTYGVSMGAVWFFER"
BASE COUNT         1475 a         1484 c         1380 g         1481 t
ORIGIN      
        1 aacggctcag acagattttc cgctcgaaat gcttcgcgct tcgcgcagca acttctttcc
       61 tcgcggaatc ggagacatgg cctggaaacg cacagtaaag attggtgtac tgggcggaca
      121 cgaaggagtc tataacccgc gcgccactgt gattttgggc gtgctcacat tcgtaatcgt
      181 ctacttcttc ccatccagtc atgcgatcga aagcatgtat ggtctggctg tgattctaac
      241 catgaacatt gggatcttcg cctacctgct cagacaaatt cgcgctcaca aaatcaacgg
      301 catgactgtc atcggcatgc tcgtgatgac agtcatgctc ttcaacattg tgtacaacaa
      361 gttttttgac ggcgcgtggt tcgtggtagt actgttgttc gcatacctcg gaatcttcgt
      421 tgtatcagaa gcgatctacg ccatctggct ggaaaaactc aacgtagtgc cgctcgaact
      481 tggtctgtgg tatcccatat tcaacgatcg caaggtagac tataaaaaca ttgttctagt
      541 ttccaagttc catcccggcg tgattcactt cttgaaaaac tatgtgaaaa gcggccacat
      601 cccccttgta gttcactttc gcacggattt tgacgaagat gtgccagcac aggttccggc
      661 ctggtacaag aacatagacg tacccgaaag cacagataca atcacagcca tcacgcgata
      721 cgtaaaacgc atgaagccac agcgggtgca tctgattcct gccctcgtgc gaggaattga
      781 tccgatcagt cgctactact ttggaaattc catggagcgt ctgaagtacg cagtctcaca
      841 attcgcagac ttacaggtgg aatacaataa agagagaatt cacatccgcg tgggcgacat
      901 tgtcaaaaga ttgttcccgc gccttggcga taggttcttc tccgatcaaa aacctacagc
      961 cgaaggttga accctttcaa cctcggaccc tatgagcgct tcgcttgcaa gcttccctta
     1021 ttagaaggga agcttgttga gtagctcttt gccaacgccc ggaatcttct gatccagttt
     1081 gtttttcaaa atatctttga cgctccctgt aagtacttcc gtcaaactcg gaagttctgc
     1141 cagaagcttc acactcgggt tcgcgaaatt tcccagggat gcaaatggca gatccacctg
     1201 tccgttctgg atcaacttgc caagaaccct gtttcgcacg tcctctacgc tctgcccttt
     1261 gagtttcttt tcaatgcccc tgtcaaatcg cgcgaggatc tcttttgatt ctgcttccgt
     1321 ggcgcgcaca gacgcgttga aattgtgttc gttcgtcgcg caattgattg tgcttccctt
     1381 cttgagcagt agatcgtagt tcgcggttgg aaacactgtg tcatccagaa acgtaacgag
     1441 tgcgtgagag tatctcactc taacatttac gtcgcgaaga agaactgctt tttcagaaag
     1501 ctttttccag gagaatccta cctgactgag aacgggaagt tcaccggcga gctgatcaaa
     1561 aatcgccata ccagtgatga aagaatcttt cttaaccacg agggcgtact ctactcctgt
     1621 actgacttcc ccggttttgg aatcaaacgg agtcacattg ccactcgagc gaatgagaaa
     1681 actcgcagcg tccgtctgtt tggcgctgat aacatttacg ttggcatcga aatcaagatg
     1741 tgcattgttg tgtttgacca gatctgccgg atcaatatcg atcgaatcaa ttactagagc
     1801 caggccgctg attcggatga gctgccccgt tttctgcatc aataaattga cagtagcatc
     1861 tttgagtccg atctcgcgca aatccgcgga tataggaagg tcttttgcgc tgaagggttt
     1921 ctcctcttct ttcgctgctg tttccgctgg cttttcagcc agcgccggat ttggctttct
     1981 gcccacaatc aacggttttt gaaacaatgg cttcagactg ttaccaccat tggcgaataa
     2041 cgtcatagaa acaaccggac tgctcagcat gaatcgattg agttcaaact ttctgcgaag
     2101 caaaggtaga aggctaaatt tcagatcaat cttgccaatc gtgacgacgg aacctttcat
     2161 aggaggcctc tccgaaagag gtgtgccctg atttgcaaaa gagtcccggg ccccgagact
     2221 gactccttcg agttccacag atgacatggc ggaaaacagg ctcacgttta gcttacgaac
     2281 ttccgctcga acgtttaagt tcgattctat ttgctgcacg aggaagtttt cattcaaaac
     2341 tactttgagg gcataccagg cactggctga aacgagcgca aggatgacaa agagcggaat
     2401 tacaacgaag tacagaatct ttttcatacg gcctcgcgcc aattctgcga cacaaagcac
     2461 gtttatggaa gcaaaaattt agaattcaag aaacgcgaaa gagttttccg agcaaggtga
     2521 acctgcgtcc aaaaatcaag cgcaagactg aaaagaaata ctgccattgc tccgatgtgg
     2581 tttcggttcg cacagaacca taccttctgt atagagacac agggatttca atgcatctca
     2641 tatggaaacg aagaatgcct atcatcattt ccgcaagata cgtgcggtcc gtggcgcgaa
     2701 gctcctctgc gattttcaag aatgaatctt tccagatggc tctataaata cagccaatgt
     2761 ctgtgaagcg tggttccaga tcccaccaca gaatctcaag aagtttgcca agggccacat
     2821 tgagccagcg atacagcggt ccaagattcg ttccctgttc catgagctgt ctggtagtgc
     2881 gcgtacccac aaccatatca ctgtctttca tgtattcgag aagtttggga agatcgttcg
     2941 cacggaaatt tccatctgca gtagccagca ccagtatgtc tccggatgcc tctctgaaag
     3001 cacggtagat gatttgcgct ccgtgaggat tgccggcaga ttctgttgcc tgaatgactt
     3061 tcgcacctgc cttgcgagca atctccacgg tgcgatccga acttcccata tccgctacaa
     3121 tgatttcatt tacacgacct ctgaagtcct gaagaacatc cggaagagtg cgctcattgt
     3181 tgaatgcagg aacgaccaga gtggtgcgga atttgtgaaa tttttctgag cgaattctgc
     3241 aggtcgcgga atagtaatcc gcaagagaat tgatattata gtatctgcct tttacgagcc
     3301 ccgcatgaac ttcgtatttg tgagacatca aatcgatcac atctgtgagt tccacgacgc
     3361 ctgatttgtt agatggaggt gtctctctga atgctttgaa gtactctgga ctaaaaatgt
     3421 aagtacctag ccccagaaga tcgttcggtg ggctgtccgg tttttccacc agcttcatga
     3481 ctcgggtgcc ctcgagttct acagagtagt tcttgcgaat ctcagacaaa agatccgatt
     3541 tcaaaacagc aatcatcgcc tctgctttct tcttctggct ccacagcttt gccagagcct
     3601 ggtggttcat atccatgtag aattcgtcgc cgagaatgac cgaaaaatcg ccgtcaatcc
     3661 tgtctccaag agcagcaatg tccgacgcaa ggccttttcc agtccattct gcagtgtgga
     3721 tcttcgcatg cggataatgg cggcggattc gctccacttc ggaaagaacc tgttccttca
     3781 aatgtccaac gatgatgtag agttcaggga ctttcaatac tttcagttgt atgtagacgt
     3841 tcttctcaag aagcgtttga ttctcaaatg tgaagagagg ctttgggacg taactcgttc
     3901 ttggatacgc ccgggtgcct ttgcccgcgg ctgcaatcac accatacttc agtggtcggc
     3961 cagacattgg tagagagggc gggagaacgg tctcggcgtc aaacacaaca aaattcggtt
     4021 ttccctcggg agccagcggc caggatgccg ggagtcatat gaaagcaacc accttcggag
     4081 aactcaagtc acagggatac agatacaggt ccgttcgcac ggaaatggct gaaaacctgg
     4141 ccgcgaaaat ccgttccaaa caacagatct ttcctgaaat ccacggttat gaagatacag
     4201 tcattccaca gttgacgcac gcgattctgt cgggtcataa cattgccatc ctcggagaaa
     4261 aaggccaggc gaaatctcgc atcatgcgct ctctaaccac tttgctcgat gacgaattcc
     4321 cggttctcac cgggacggaa attcctgaga gcccttttca tcctatcacc gcactcggaa
     4381 gacagcacgt cgctgccggc gactccgctc cgattcgctg gctttcgcgc gatgaacgct
     4441 acggagagag acttgctcct ggaagcagaa tcgcagacat cattggagac ctggatcctt
     4501 cgcgcatcat tgccggagaa tccctgggaa gcgaaagcgc tcttcactac ggacttctgc
     4561 caaggatgaa tcacggaatc ttcgccatca atgaactgcc ggatctcgat tatctggtgc
     4621 aggtcgctct cttcaacgtt ctagaagaag ccgacatcca gattcgcggc ctacccattc
     4681 gaattccact cgatgtattt gtatgcttca ccgcgaaccc tgcagactat tccagatcag
     4741 gaaaaatcat cacacagctg aaagatagaa tcggagctga aattagaact cactatccga
     4801 aagcgcgttc cgttggtctg gccatcactc gccaggaaat ccaaaacctg atcgtagatc
     4861 cacgcatcat cgtgccggag ttcatggaaa acattattga agagatcaca atccaggcac
     4921 gcatgtctcc agtggtgaac cagaagtcag gcgtttctgc gagattgagc attgcaaact
     4981 atgaaactgc aaccgcttcg gccagaagac gtgcccttct cctcggagaa aatcccggcc
     5041 atgtaagact cacggacctg ggcaacctct ttgcttctag ctccgggaag atcgaactcg
     5101 atccataccg cgatgaagct gtgactgagt ttcaggtgat tcagaaaatc atagatgagg
     5161 caatccgcga agtcttcctg gagtactttc ctctaaagaa gtatgagcca accatggatc
     5221 aagtcgccaa acaaatcttc gaagcaaaag gcctcgaaat atcagacaca atgaatgtgg
     5281 aatcgtatcg cgacattctg aaaaaagttc cggccctgtt tgactttatg cgggatcgag
     5341 ggtgggactc ggattcgcag actcttgcat gcggagtgga gttcatcctg gaaggcctcg
     5401 cagtgaatca gaagctatcg agaagaaagc tcggtgaaat cgtatcgttc aaatcagtgg
     5461 acatctactg agccgggatc ggcttttgga ttgatccctg agtcgattct gacagttttg
     5521 cctggatgaa acagcacacc cgaggcctgg gcctttactt cattgcctcg ggcacggccg
     5581 ccgcggtgaa ctttggatcg cgttttgtat atgattcatt cttcacattc gggacgagtg
     5641 tagtgcttgc ctattgcacc ggcatgttcg tcaacttttc gctcagcaag ctctttgtct
     5701 tcgacgcacg cacttcgggt agaacctggc gcgaattcac aaagttcatc gcagtggctt
     5761 ccctggggct cgtagttacg tacggcgttt ccatgggcgc ggtctggttt ttcgaacgat
//
LOCUS       DAORFF010000204         5814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7420_length_5814_cov_3.437055, whole genome shotgun sequence.
ACCESSION   DAORFF010000204 DAORFF010000000
VERSION     DAORFF010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7420_length_5814_cov_3.437055"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PKK76_09495"
     CDS             complement(<1..614)
                     /locus_tag="PKK76_09495"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNL68748.1"
                     /translation="MSRFRLLSYALTLFLLLSACTKNSSPRLIVVISIDQFRAEYLER
                     FKDLYLPAHSLGKPGGFRYLMEKGAYFRNAHHDHFPLYTAPGHSVLLTGAPPYKTGII
                     GNDWYDRKMGRKTYCVEDCNFKLIGVKNQSCIASDPAFKPGVSAKNLHASTVGDQLKL
                     ATGAHAKVWGLALKDRAAVLLAGHAPDGVLWFDEDTGGWISSTA"
     gene            complement(666..1265)
                     /gene="phoU"
                     /locus_tag="PKK76_09500"
     CDS             complement(666..1265)
                     /gene="phoU"
                     /locus_tag="PKK76_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNL68749.1"
                     /translation="MLLDTKLKELRTEVAIMARRAEAMLEKATDLLHAPTEEGVKEVL
                     AMDATLNDLEIKMDDLCMRILALDEPYANDFRFVFSLIKTTRDLERIGDESKTVAKWS
                     QRITGEVPPRLLELGKKARETLNAAIHCLVNSDVSLTGTVMDLELTTDELEDEIINAN
                     PSIPIAFIAKALERVGDLSANIAENVVFSVKAEEIRHKT"
     gene            1434..2411
                     /locus_tag="PKK76_09505"
     CDS             1434..2411
                     /locus_tag="PKK76_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNL68750.1"
                     /translation="MNQSRAMLATIPLAAALFLTSACKERKIISIDGSSTVGPISMAV
                     AEDFQHANGNVKVTVGISGTGGGFKKFCNGETDISDASRPIKKEEVEACKTKAIEYVE
                     LPVAYDGLAVLVSPQNDWVKELTTEQLKKIFTSENPAKTWKEVNPAWPDAAIKIFAPG
                     KDSGTHDYFVEEILGKKKQMRPDASFSESDNVLVTGISGEKNSIGFFGLAYFENNKDK
                     LKLVPIVNPKTKAAVLPSVDTVKSGEYAPLSRPLFIYVSTKAAAKEEVKAYIDFYLTN
                     GAKLSAQVGYIALPDGVYTKAKSRFASLQTGSVFQMEGHEGKTIEQVFN"
     gene            2461..3411
                     /gene="pstC"
                     /locus_tag="PKK76_09510"
     CDS             2461..3411
                     /gene="pstC"
                     /locus_tag="PKK76_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNL68751.1"
                     /translation="MSDLLTQAVRGNRRDVFSRYGERPSYGVKERFVRYTLRTFSYFT
                     VFVTLAIVFILVIDASEFFSQASFLEFITGREWLPFGEPKKLGVLPLLTGTVMIAVGS
                     SLVSIPIGLGIAIFLTQYTSRRFQRFMGPVLEVLGGIPTVVYGFFALTTLTPLLKHLF
                     TSIDTFNALSAAIVVGLATLPMVASLSADALQVVPSSIRNAGYALGMSKFHVVTRIMV
                     PAAFSGIVASFLLAFARAVGETMAVSIAAGSTPSMSFDYLKSIQTMTAFIVQISMGDT
                     AAGSIEYYTIYAIGISLFVLTFAFNFAATAIIRRFREVYQ"
     gene            3408..4271
                     /gene="pstA"
                     /locus_tag="PKK76_09515"
     CDS             3408..4271
                     /gene="pstA"
                     /locus_tag="PKK76_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNL68752.1"
                     /translation="MKDLRVRYRIYGAIFEGFCFSANVLAFGFLLYLLGSVLFQGVPN
                     INWTFLTEYPSRFPQKAGILAAIAGTVWMMVLTAFVSIPLGIGAAIYLEEYARPGRLV
                     NLVRLNIQNLAGVPSIIYGMLGLTMIVRGLGLGRSILAGALTMALLILPVITIATQEA
                     LRAVPGSLRLAGFGIGMTRWQVVRYQVFPSALPGIMTGIILALSRAVGESAPLLLVGA
                     TAYSAYLPSSPMDGFTVLSVQIYNWIDQPQKEFQALAAGAIVILLVLLIALNSIAIYL
                     RQYYRKKVSTG"
     gene            4276..5079
                     /gene="pstB"
                     /locus_tag="PKK76_09520"
     CDS             4276..5079
                     /gene="pstB"
                     /locus_tag="PKK76_09520"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNL68753.1"
                     /translation="MQQITQKIAAPENGDATRTEAVFACSNVNIHYGDFHAIQDLSLD
                     LLENRVTALIGPSGCGKSTFLRSLNRMNDFIDSFRISGSLLLDGEDIYAPDVDPVEIR
                     LRVGMVFQSPNPFPKSIRENVALGPRINGYKGNLAELVEEALVKSYLWDEVKDKLDQS
                     AYSLSGGQQQRLCIARAIAMQPEVLLMDEPTASLDPISTKRIEELIVELKKSYSIVIV
                     THNMQQAARIGDFIAFFYQGKLIEYDSTFRMFTNPGQKLTEDYITGRFG"
     gene            complement(5080..>5814)
                     /locus_tag="PKK76_09525"
     CDS             complement(5080..>5814)
                     /locus_tag="PKK76_09525"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL68754.1"
                     /translation="RTRELTAELQIAKRIQSSILPESNPQIEGLRIESAYRPASEVGG
                     DFYDFHTEGKMVGLIVADVCGHGVGAALIASMVKISFANLRSHWKEPDEILRGLHRSM
                     SPRIRGQFITACYAFVDMNMRRLYFSSAGHPPVLIWRRREKRLHELGMRGPVIGWPGD
                     WTCPSVSWDLEPGDRLVLYTDGIFEQRNQKGEMFGFDRFYESISATSELTTKDAADAI
                     LSAVDSWHKKEDQLEDDVTLILLDIE"
BASE COUNT         1381 a         1439 c         1409 g         1585 t
ORIGIN      
        1 tatgcggtgc ttgagatcca cccgccagta tcttcgtcga accacaatac gccgtccgga
       61 gcatgcccgg ctagaagtac agccgctctg tccttgagtg caagccccca gaccttcgcg
      121 tgcgcacccg tggccagctt tagttgatcc ccgaccgtgc ttgcgtgaag gttttttgcg
      181 gatacgcccg gcttgaatgc aggatcgctt gcgatgcaag attggttctt cacgccgatg
      241 agtttgaagt tgcagtcttc gacacagtag gtctttctac ccatcttcct gtcgtaccaa
      301 tcatttccta tgattccagt tttgtaaggt ggcgcacctg tgagcaaaac agaatgtccc
      361 ggtgctgtat agagagggaa atgatcgtgg tgcgcattgc gaaagtaagc acccttttcc
      421 attagatagc gaaagccgcc gggttttccg agagaatgcg ccggaagata cagatctttg
      481 aatcgctcga ggtattctgc gcgaaactgg tcaatcgaga taactacgat caggcgcgga
      541 ctggaattct tcgtgcatgc agaaagaagt aaaaaaagcg taagtgcata gctcagcaaa
      601 cgaaaccggc tcatcctgag aggatgaatc gctttcgtgc cagttctgtg acgaatggaa
      661 tacttctatg ttttgtgacg aatctcttcc gcttttacgg agaaaactac gttctctgct
      721 atgttcgcgg agagatctcc cacacgttcg agcgcttttg caatgaatgc gatgggaatg
      781 gaaggatttg catttatgat ttcatcttcc aattcatctg tagtaagttc cagatccatg
      841 acagtgccag tcagcgaaac atcgctgttt acgaggcagt gaatcgctgc attgagagtc
      901 tctcttgcct tctttccgag ttccaggagt cgcggcggaa cttctcctgt aattctctga
      961 gaccatttcg caaccgtctt ggactcatcg ccgattcttt ctagatcacg cgttgttttg
     1021 atcaggctga atacgaagcg aaaatcattg gcatacggtt cgtccagagc aaggattcgc
     1081 atgcatagat cgtccatttt gatttcgaga tcgttgagag tcgcgtccat ggcgaggact
     1141 tctttgactc cctcttcagt aggggcgtgc agaagatccg tcgccttttc gagcattgcc
     1201 tccgcccggc gagccataat ggccacctct gtgcgaagct ctttgagttt tgtgtccaga
     1261 agcataaatg actccgcctc gactgtcggt acagttcttc acaggtcaac ccattctgct
     1321 ctggaagggt ccaaaaacca gcttccgtag ttctagcgtc atagaattgt aacataactc
     1381 ctggatatta cgttcatgtt aaccgggaca aaaccggatc ccaggaggaa tccatgaacc
     1441 aatcacgagc aatgctcgct actattccgc tcgccgccgc tctctttctt acgtcggctt
     1501 gcaaagaaag gaaaatcatc tctatcgacg gatcgagcac agtcggtcct atttcaatgg
     1561 cagttgctga agactttcag cacgcaaacg gaaatgtgaa agtaactgtt ggcatttctg
     1621 gaaccggcgg cggatttaaa aaattctgca acggcgaaac tgatatttca gatgcttccc
     1681 gtcccatcaa gaaagaggaa gtagaagcgt gcaagaccaa agctatcgaa tacgttgagc
     1741 ttccagtggc gtatgacggt cttgcagttc ttgttagccc tcagaacgat tgggtgaagg
     1801 aacttacaac ggaacagctc aagaagattt tcacttctga aaatccagca aaaacatgga
     1861 aagaagtgaa tccagcatgg ccagatgcag cgatcaaaat tttcgcacct ggaaaagact
     1921 caggaacgca cgactacttt gtagaagaaa tcctcgggaa aaagaaacaa atgcgtccgg
     1981 atgcttcttt cagcgagtct gacaatgttc tggtaactgg aatctcaggc gaaaagaatt
     2041 caattggatt ctttggtctg gcatacttcg aaaacaacaa agacaaactc aagcttgttc
     2101 cgattgtaaa tccaaagacc aaagctgcag ttcttccttc tgttgacaca gtcaagagcg
     2161 gcgaatatgc tcctctttcg cgtccactgt tcatctacgt gagcacaaaa gcggccgcaa
     2221 aagaagaagt gaaggcttac attgatttct atctcaccaa tggagccaaa ctttccgctc
     2281 aggtgggcta cattgcactg ccggatggag tctatacaaa agcaaaatct cgtttcgcga
     2341 gtctccagac tggatctgtt tttcagatgg aaggccacga aggcaagacg atcgagcaag
     2401 tttttaattg aacaatcgca ggcggacccg tttctgggtc cgctcgcact ttattgaacc
     2461 atgagtgatt tacttaccca ggccgttcgc ggcaatcgca gagatgtttt ttcccgttat
     2521 ggggagaggc cttcttacgg ggtaaaggaa cgattcgttc gctatactct tcgcaccttc
     2581 tcgtacttca cagtctttgt tactctcgca attgtattca tactcgtcat cgacgcatcc
     2641 gagttttttt cccaggcaag ctttctagaa ttcatcacgg gtagagagtg gctgccattt
     2701 ggagagccaa agaaactcgg cgtgcttcct ttgcttactg gcactgtgat gattgctgtt
     2761 ggatcctcgc tggtttccat tcccattgga cttggaatcg ccatctttct cacgcagtat
     2821 acatcgcgca ggtttcagcg tttcatgggg cctgttcttg aggttctcgg tggaattcct
     2881 acagtggtat atggtttctt cgcgctcacc actcttactc ctcttctcaa acatctattc
     2941 acaagcattg atacattcaa cgcactgtct gctgccattg tggtggggct ggcaactctt
     3001 cccatggttg catctctctc tgcggacgcg ctgcaggtgg tcccttcttc gattcgcaat
     3061 gcaggatatg cgctgggtat gtccaagttt catgtggtga ctcgaatcat ggtgcccgcc
     3121 gcgttttctg gaattgttgc ctctttctta ctcgcgtttg ctcgagctgt gggagaaact
     3181 atggcggtga gcattgctgc tggttccacg cccagcatgt cttttgatta tctcaagagc
     3241 atccagacca tgaccgcatt cattgttcaa attagcatgg gagatacggc tgccggtagt
     3301 atagaatact atacaatcta tgcgattggc atttcgctct ttgtcctcac gtttgcattt
     3361 aactttgccg caacagccat catccgcaga tttcgggaag tgtaccaatg aaagatctgc
     3421 gcgtgcgata tcgaatctat ggagccattt tcgaaggatt ttgtttttcc gcgaacgtgc
     3481 tggcgtttgg ctttctgctg tacttgctcg gctctgttct cttccagggc gttccgaaca
     3541 tcaactggac tttcttaact gaataccctt ccaggtttcc gcaaaaagca gggatcctcg
     3601 cggcgatcgc aggcactgtc tggatgatgg tgctgactgc attcgtgagc atcccgcttg
     3661 gaattggcgc ggctatctac cttgaggagt atgcacggcc cggtagactt gtgaatctgg
     3721 ttcgcctcaa catccagaat cttgcgggcg ttccatctat catttacggt atgcttggct
     3781 tgaccatgat cgttcgcggt ctgggtctgg gcagaagcat tcttgcgggt gccctcacca
     3841 tggcgcttct gattcttccg gtcattacca tcgcaacgca ggaagctctt cgcgcagtcc
     3901 ctggttcact tcggttggct ggattcggga ttggaatgac tcgctggcaa gtagtgcgct
     3961 accaggtgtt tccttctgcg ctcccaggaa ttatgaccgg aatcattctt gccctgtcgc
     4021 gcgcagtggg ggaatcagcc ccgctcttgc tcgtcggtgc gaccgcgtat tctgcctatc
     4081 ttccatcgag tcctatggat gggtttacgg ttctatcggt tcagatttac aactggattg
     4141 atcagcctca aaaagaattc caggcccttg cggccggtgc cattgttata ttactcgttt
     4201 tactcattgc tctcaattca attgcaatct atctgcgaca atactatcgc aaaaaagtga
     4261 gcacaggata atcgtatgca gcagatcact cagaaaattg cggcacctga aaatggcgac
     4321 gcgactcgca cagaagcggt gtttgcgtgt agcaatgtga acattcacta cggtgatttt
     4381 catgcgatcc aggatctttc tctggacctc cttgagaatc gagtgacagc attgattgga
     4441 ccatcgggtt gcggaaaaag tacgtttctt cgcagtctaa atcgcatgaa cgatttcatc
     4501 gattcgtttc gtatttcagg gagtcttttg ttagacggcg aagacatcta tgcacctgac
     4561 gtggaccctg tggaaattcg tttgagagtt ggaatggtat ttcagagtcc gaatccattt
     4621 ccaaaatcga ttcgcgaaaa cgtggctctt ggcccgcgca tcaatggtta caagggaaac
     4681 ctggccgaac ttgtggaaga agcgctggtc aaatcctacc tctgggatga agtaaaagac
     4741 aagctcgatc aaagcgccta ctcgttgtca ggtggtcaac agcaaaggct atgcattgca
     4801 cgcgccatcg ccatgcagcc agaagttttg ctaatggatg agcctacggc ttctctcgat
     4861 ccaatttcaa ccaagaggat tgaagaactg atcgtagagc tcaagaaaag ctattctatt
     4921 gtaattgtga ctcataacat gcagcaggcg gcccgcatcg gcgattttat tgcgttcttc
     4981 tatcagggta agttgataga gtacgattcc acctttcgca tgtttacaaa tccaggtcag
     5041 aaactcacgg aagactacat cacaggcaga tttggctgat cactcaatgt ccagaaggat
     5101 gagagtgacg tcatcttcca gctgatcttc tttcttgtgc caactatcca ctgctgatag
     5161 aatcgcatcc gctgcgtctt tcgtcgtgag ttctgacgtg gcggaaatgg attcatagaa
     5221 gcgatcaaac ccgaacattt cccctttctg atttcgctgc tcaaagattc catctgtgta
     5281 aagcaccaga cgatctcctg gttccaggtc ccagcttaca gaagggcaag tccaatctcc
     5341 tggccaacca atgacagggc cgcgcattcc cagttcgtga agacgctttt ctcttcttct
     5401 ccagattaga acgggtgggt ggcctgcgga agaaaagtaa agcctgcgca tattcatatc
     5461 tacaaacgcg tagcatgctg tgatgaactg gccgcgaatg cggggagaca tggaacgatg
     5521 aagccctcgc agaatttcgt caggttcttt ccagtgcgaa cgaaggttcg caaatgaaat
     5581 ttttaccata gacgcaatga gcgcggcacc cacaccgtgc ccgcatacgt ctgcgacgat
     5641 cagtcccacc atttttcctt ctgtatgaaa atcgtagaag tctcctccta cttcgctcgc
     5701 agggcgatag gcgctttcga ttctcagtcc ctcgatttgc ggattcgatt ctggaaggat
     5761 ggagctttga attctttttg caatttgtag ttccgctgtg agctctcgag tgcg
//
LOCUS       DAORFF010000205         5810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7426_length_5810_cov_3.662554, whole genome shotgun sequence.
ACCESSION   DAORFF010000205 DAORFF010000000
VERSION     DAORFF010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7426_length_5810_cov_3.662554"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKK76_09530"
     CDS             complement(<1..238)
                     /locus_tag="PKK76_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNL68755.1"
                     /translation="MEILRVLSEARAPLTARDVHTRVKKKFSHVTLDTVYRNLLMLTG
                     TGFISHINLQNKGTARFEFQAGHHHHHAICLECGA"
     gene            505..1047
                     /locus_tag="PKK76_09535"
     CDS             505..1047
                     /locus_tag="PKK76_09535"
                     /inference="COORDINATES: protein motif:HMM:NF022439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HNL68756.1"
                     /translation="MNRIPLTISIAFLTILVPIFAEPHREHGAHEHGIASLDVTVDGN
                     KVDIDLHSPAVNIIGFEHSARTAADKARQQAGLDRIRKNLSVLFQFDPSRGCKIKIKD
                     LKIETEQHADHPGEKTGEHSELDGEFEVTCAKSPAGSKLKFAFTKIFRDFNLTKIRVQ
                     VMSDHPVSTVIENDEGSVEL"
     gene            1044..1796
                     /locus_tag="PKK76_09540"
     CDS             1044..1796
                     /locus_tag="PKK76_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNL68757.1"
                     /translation="MNAIEIKDLRFAYSHGPEILEMPEFHVRQGEKVFLFGPSGSGKT
                     TLLSIITGILSARSGRVSVLGEDLTRLSASARDRLRGTRMGYIFQMFNLLPYLSVIDN
                     ILLPCKMNPHRAERVGADVEAEAWIIAEHLGIAEHAHKKPSDLSVGQQQRVAAARALL
                     GNPDLIIADEPTSALDTDHRESFLELLFTNTKKSGAAVLFVSHDRSLARLFDRSISLP
                     DLNLAAPSVSPAIKKPATKVTRTSSGKRVQKK"
     gene            1793..3055
                     /locus_tag="PKK76_09545"
     CDS             1793..3055
                     /locus_tag="PKK76_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNL68758.1"
                     /translation="MNYIRLTLHSLKNRALTTSLTVLSIGLSIGLFFGVERVRTAARE
                     SFEGTISGAQLLVGARTGGVQLLLYSIFHIGDSTNNVSYQSFKDWSGRKGVLWTIPFS
                     LGDSHRGYRVVGTNENFYKYYKFRDSRSLEFTSGAPNADLFDVVLGSEVAEKLHYGLG
                     EKVLVSHGVAAKTLESHEDKPFTVRGILKRTGTPIDRSLFISLEGMEAIHIDWKGGYA
                     PSEEERISADRVRKMKIKVDTVTAFLVGLQNRSQVLTFMRNVNDYKEEPLTAVVPAYA
                     LQQLWRIVGYAEDALRIISVFVVLVGITGMILSIYTTLNERRREMSILRSLGARSHHI
                     LLLLVSEAFLLTVAGCITGIVFVYALLGGLQPIIEDQFGIFIPVQMPTLMEFGYLGCV
                     LAAGILAGLIPAWTAYRNALHDGLSVRV"
     gene            3135..4346
                     /locus_tag="PKK76_09550"
     CDS             3135..4346
                     /locus_tag="PKK76_09550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNL68759.1"
                     /translation="MMRWKPVLLLFAIFGFLAVAFALFFGALSIFDSPLYAFRLLAYG
                     NSDTDDFKIFAERRIEKASLPSFIERTTLDIPAQVSLPYSGEQRTENIEEFLKKADTR
                     AFLVIKDDRIAVEKYWNSSRDSINTSFSVVKSFDSAMIGAAISDGLIGSVDDPVIEYI
                     PEIAGRGLDQLTIRNLIRMDTGIEYKHNFEIPFFRIPFGDDARTYYGGNLHRVAFSVR
                     PSASPIGPAFRYNNYHPLLEGIIIERVTRMPVAKYLEQKIWKPMGAEYDASWSLDQSD
                     GFEKMESGLNARAIDYARFGLLYLHQGKWNGKNILPAEWVRISTTPDSPERPFDTFQG
                     WQKLGGYYAYHFWGLHAPNNQNDFMAMGNYGQIIYVAPRKNIVIVRLGSRDSQTIPFG
                     VLFRELVNQIR"
     gene            4354..>5810
                     /locus_tag="PKK76_09555"
     CDS             4354..>5810
                     /locus_tag="PKK76_09555"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNL68760.1"
                     /translation="MLKTIHTDPGLLPFRPRILRRYARIQEVQDRITNGLSLTDFASG
                     YLYFGMHTTAEGWVFREWAPNASEMFLVGDFSQWQIREEFRVHKHGSNWEIQLGHTQI
                     QPGQLYKLWMRWPGGEGFRLPSYGRRMVQDANTKIFTAEVPHREEYKWKVQNFDRGRT
                     PPFIYEVHVGMSSEEEKVSTFNEFRLNILPRIHRLGYNTIQMMAVQEHPYYGSFGYHV
                     SNFFAVSSRFGTPEELKQLIDEAHSLGIAVIMDLVHSHSVKNIDEGLALFDGTPFQYF
                     HDGPRRLHSAWDSLCFNYSKPEVIHFLLSNCKYWLEEYKFDGFRFDGVTSMMYIHHGM
                     GVDFMRYEMYYDGNQDEDAITYLALANKLIHAINPNAITIAEDVSGMPGLASSVENGG
                     YGFDFRLAMGIPDYWIKVIKELPDEKWNVGNIYYEMTNHRAEEKTIAYSESHDQALVG
                     DKTIMFRLTDAEMYFAMHRQAQSLVIDRAMALHKM"
BASE COUNT         1548 a         1434 c         1406 g         1422 t
ORIGIN      
        1 ccgctccaca ctctagacag atggcatgat ggtggtgatg tcccgcctga aattcaaagc
       61 gcgccgtacc cttgttctgt aaattgatat gactgataaa tccggtgcct gtaagcataa
      121 gaaggttgcg gtacacagta tccagggtta catgcgagaa tttttttttc actcgagtgt
      181 gtacgtcgcg tgcagtcagg ggcgcccttg cctcagacaa gactctcaga atttccatcc
      241 gctgcggggt cagcttctgg ccatttttcc tgagagattg aaaacattgt tccagcgtat
      301 acatagaaga ctcggcttag atggactccc taaccgaacg atcgtcaagt ataaatcctt
      361 tttttgtccg catagacacc gggcgctcgt attaaacaaa gtacgtgggc agatgtgttg
      421 tttccccgga tagcacccag gaatatttct agacaagaaa atacttattt acaaatatac
      481 gactctggag taaaatcaat gacaatgaat cgtattcccc tgaccatctc tattgcattt
      541 ctcaccattt tggtgcccat atttgcagaa cctcaccgtg agcacggggc ccatgagcat
      601 gggatcgcct ctctggatgt caccgtggat gggaacaaag tggatattga cctgcattct
      661 cccgcagtga atataattgg atttgaacac agcgcacgca ctgcagcaga caaagccaga
      721 cagcaggccg gcctggaccg gattcgcaag aatctgtcgg ttcttttcca gtttgaccct
      781 tcgcgcggct gcaaaatcaa aatcaaagac ttgaagattg aaacagaaca gcatgcggat
      841 catccggggg aaaaaacagg ggaacatagt gaactggatg gggagttcga ggtgacttgc
      901 gcgaaatcac ccgcgggttc aaaattgaaa ttcgccttca cgaagatatt ccgggatttt
      961 aatctgacaa aaattcgtgt acaagtgatg tcggatcatc ctgtgtccac tgtcatagaa
     1021 aacgacgagg gaagtgtaga gctttgaatg caattgaaat aaaggacctt cgcttcgcat
     1081 acagccacgg tcctgaaatt ctagaaatgc cagagtttca cgtgcgccag ggagaaaagg
     1141 tatttctctt tggcccaagc ggcagcggaa agaccactct gttgtccatc atcactggaa
     1201 ttctatccgc ccgttccggt cgcgtttccg tcctgggaga agatctcacc cggctcagcg
     1261 cgtctgccag ggaccgactg cgcggcactc gaatgggata tatttttcaa atgttcaatc
     1321 tacttcctta cctttctgtc atagataaca ttctattgcc atgcaaaatg aaccctcatc
     1381 gcgcagagag agtgggcgcg gacgtggagg cggaagcatg gatcatcgca gagcacctgg
     1441 gaatcgcaga gcacgcgcac aagaaaccat ccgatctcag cgtgggtcag cagcaaagag
     1501 tggccgcagc aagagcactt ctcgggaatc cagatctcat catcgcagat gaacctactt
     1561 ccgcgctcga cacggaccat cgcgaaagct tccttgagct tttattcacg aatacaaaga
     1621 agagcggagc ggcggttctg tttgtgagcc acgatcgatc ccttgccaga ttgtttgacc
     1681 gttcgatttc attgcctgat ctaaatctgg cggcaccttc tgtaagcccg gccatcaaaa
     1741 aaccagccac gaaagtaacc agaacctcat ccggcaaacg ggtacaaaag aaatgaacta
     1801 cattcgcctg acacttcatt cactcaagaa tagagcgctt acaacttctc ttactgtgct
     1861 ttcgattgga ctatccattg gattgttctt tggagtagag cgagtaagaa ctgcggccag
     1921 agaaagtttt gaaggcacaa tcagcggcgc acaactcctg gtaggcgcac gaacaggcgg
     1981 agtccagctc ctgctctatt ccatctttca tattggagac tccactaaca atgtcagtta
     2041 tcagtcattc aaagactggt cggggagaaa aggtgtactg tggaccatcc cattttcgct
     2101 cggagatagc catcgcggct atcgcgtagt cggcacaaac gaaaacttct ataaatacta
     2161 caaatttcgt gatagcagat cactggagtt tacttccggc gctccgaacg cggatttatt
     2221 cgacgtagtc cttggatctg aagtggctga aaagttacac tacggactcg gagagaaggt
     2281 actcgtttct cacggagttg cggccaaaac cctggaatcg cacgaagaca aaccctttac
     2341 agtgcgagga attcttaagc gcacgggcac tccgattgat cgatctttgt tcatcagcct
     2401 ggagggaatg gaagcgattc acatcgactg gaagggcgga tacgctccat cagaagagga
     2461 acgcatcagc gcagaccggg tgcgcaaaat gaaaatcaaa gtggataccg tgactgcgtt
     2521 tctggttggt ctgcaaaaca gaagtcaggt tctcactttt atgcgaaacg tgaacgacta
     2581 caaagaggag ccactcacag cagtggttcc ggcctacgct ctccaacaac tctggcgaat
     2641 cgtgggatac gcagaagatg cactgcgaat catcagcgtc tttgtggtac tcgttggaat
     2701 taccggcatg attctttcta tatataccac tttaaacgaa cggagaagag aaatgtccat
     2761 tcttcgttct ctcggggcaa gatcgcatca tattcttctt ctgctcgtat cagaagcgtt
     2821 cctacttacg gtcgccggtt gcatcacggg aattgttttc gtttacgcgc tgttaggtgg
     2881 actgcaaccg atcattgaag atcagttcgg gatcttcata cccgtgcaaa tgccaacgct
     2941 tatggagttt ggctatctgg gatgcgtcct ggctgcaggc attctggctg gcctcatccc
     3001 cgcctggact gcatacagga atgctctaca tgacggtttg agcgttcgag tctaaagatc
     3061 ttgcatcttt ttgaccgcgc gggccgcatc taaaaacggt tgcgcacact gaagaggacc
     3121 atcacactcg gctcatgatg cgatggaagc cggtcctctt gctgttcgcg atcttcggct
     3181 ttctagcagt ggcctttgct ctgttcttcg gggccctttc cattttcgac tctccactct
     3241 atgcattcag actcctggcc tatggaaatt ctgacacgga cgacttcaag atattcgcgg
     3301 agaggagaat tgaaaaggcc tcactgcctt ctttcattga gcgaacaact ctcgacatcc
     3361 ctgcgcaagt atccctgccc tatagtggag aacaaagaac agaaaacata gaagagtttc
     3421 tcaaaaaagc agacacacgt gcattccttg tgatcaagga tgatcgcatc gcagttgaga
     3481 aatactggaa ctcttcgcgg gactccatca atacttcttt ctccgtagta aaatcatttg
     3541 attcagccat gattggagcg gctatttcgg atgggctgat cgggtctgtg gacgaccctg
     3601 taatagaata cattcccgaa attgcaggca ggggactcga tcagttgacc attcgcaatc
     3661 tgattcgaat ggacactgga atcgaataca aacataactt cgagatcccc ttctttcgca
     3721 ttccatttgg agatgacgcg cgcacttact atggagggaa tctccatcgc gttgcattca
     3781 gcgtgcggcc gtctgccagt ccgattggcc cggccttccg atacaacaac taccatccac
     3841 tgcttgaggg catcatcatc gaacgagtca ctcgcatgcc ggttgcgaaa tatctggaac
     3901 aaaaaatttg gaaaccgatg ggtgcagaat acgacgcgtc ctggagtctg gaccagtctg
     3961 acggctttga aaaaatggaa agtggactca acgcgcgcgc gatcgactat gcacgatttg
     4021 gtcttcttta tctgcatcag ggcaaatgga acggcaagaa cattcttccg gcagaatggg
     4081 ttcgaatctc tactactcca gattcaccgg aaagaccgtt tgatacattt cagggctggc
     4141 agaagctggg tggctactat gcgtatcatt tctggggctt gcatgccccg aacaaccaga
     4201 acgatttcat ggctatggga aactacggac agatcatcta cgtcgctccc agaaaaaaca
     4261 ttgtgattgt aagactcgga tcgcgcgaca gccagaccat tcccttcgga gtgttgtttc
     4321 gcgaactggt aaaccagatc cggtaggcgc gacgtgctca agaccattca cacggatcca
     4381 ggactgctgc catttagacc gcgcattctc agacgttatg cgcgcatcca ggaagtgcaa
     4441 gatcgcatca caaacggtct ttctctcacc gactttgctt ccggatacct gtactttggg
     4501 atgcacacca cagcggaagg atgggtcttt cgagaatggg cgcctaacgc ctcagaaatg
     4561 tttctggttg gcgatttttc gcagtggcaa atccgcgaag agttcagagt ccataaacat
     4621 ggatccaact gggaaatcca attgggtcac actcaaatcc aaccagggca gctttacaaa
     4681 ttgtggatgc gatggccggg cggagaagga tttcgccttc cttcttacgg aagacgcatg
     4741 gtgcaggatg caaataccaa aatattcacc gcagaggttc cacaccgcga agagtacaaa
     4801 tggaaagtgc aaaatttcga tcgcggaaga actcctccct ttatttacga agtgcacgtt
     4861 ggaatgtctt ctgaagaaga aaaggtttct accttcaacg aattcagact gaacatactt
     4921 ccgcgcattc acaggctggg ctacaatacg atccagatga tggcggtgca ggaacatcct
     4981 tactacggat cattcggata tcatgtgtct aatttcttcg cagtatccag cagatttgga
     5041 actccggagg aactcaagca attgatcgat gaagcgcatt ctcttggcat cgcagtcatc
     5101 atggatctgg ttcactctca ctctgttaaa aacatagatg aaggactcgc tctgtttgac
     5161 ggcacaccct ttcaatattt tcatgatggc ccgcgcagac tacactcggc ctgggactct
     5221 ctgtgtttca attattcaaa accagaagtc atccatttcc ttttgtccaa ctgcaaatac
     5281 tggcttgaag agtacaaatt tgacgggttt cgcttcgatg gagttacttc gatgatgtac
     5341 attcatcatg gaatgggtgt ggatttcatg cgatacgaaa tgtattacga tggcaatcag
     5401 gatgaagatg caattacgta cctggccctg gcaaacaagc tcatccacgc catcaaccca
     5461 aacgcaatca caatcgcgga agatgtaagc ggtatgcctg gacttgcgtc ctcagtggaa
     5521 aatggcggct atggattcga tttcagattg gccatgggaa ttccagacta ctggatcaaa
     5581 gtgatcaaag agttgccgga cgaaaaatgg aacgttggta acatctacta cgagatgacc
     5641 aatcacagag cggaagagaa aacaatcgca tatagcgaat ctcacgatca ggcactggtg
     5701 ggagacaaaa ccatcatgtt tcgcctgaca gatgctgaaa tgtatttcgc catgcaccgt
     5761 caggcgcaaa gcctcgtgat cgatcgtgcc atggccctcc acaaaatgat
//
LOCUS       DAORFF010000206         5799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7451_length_5799_cov_3.918872, whole genome shotgun sequence.
ACCESSION   DAORFF010000206 DAORFF010000000
VERSION     DAORFF010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7451_length_5799_cov_3.918872"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKK76_09560"
     CDS             <1..475
                     /locus_tag="PKK76_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68761.1"
                     /translation="LRSSAGLNLGIDFLPGAFPFAPPIARALAADIVWFDSLVMNVDR
                     TAKNPNLLRWHNQVWIIDHGACLPAQHGDDPLSDAALREFPRIADHVLLKHAGSIADA
                     HSRLAPLLSAEMIRDAAALVPDSWFIKHGREEYIDFVQKRLSSGPFAEEAERVRS"
     gene            462..851
                     /locus_tag="PKK76_09565"
     CDS             462..851
                     /locus_tag="PKK76_09565"
                     /inference="COORDINATES: protein motif:HMM:NF022681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HNL68762.1"
                     /translation="MSDREAYQYAMWRVVPLLERGEFINAGVVLFSRRHRFLKAQVHL
                     DEVRLKAICPDVDLENVRSQLTFREKIAAGDPEGGSVALQSQSERFGWLVAPASTVIQ
                     PSQVHTGLCDDPEAALSHLFKRLVMIA"
     gene            complement(848..1609)
                     /locus_tag="PKK76_09570"
     CDS             complement(848..1609)
                     /locus_tag="PKK76_09570"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNL68763.1"
                     /translation="MTAAAAYRKTIHKCARVIESTFYDLHRKGQKENLHEFRLAVKRL
                     RALLPSLPSPPSLVLLSPAFRAAGRIRNIDVQRSILEETLDGRRMPDNLKQFLKRRRK
                     KARRRLRTITRMIPADTLEDLVRAIQRHTNQEPDTGLEESIRKNIEGRITDVNESASQ
                     TMNAEQIHALRAKVKEAAILSMLLPPGSTPLVPFEKTADLLGKWHDCVVTLKTLDRFR
                     KKHLEAELKNLPDLVDRMKDRYFASAMSLMNAPPR"
     gene            1725..2969
                     /locus_tag="PKK76_09575"
     CDS             1725..2969
                     /locus_tag="PKK76_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HNL68764.1"
                     /translation="MLRSRLVSGFLIVVGALLAAEIGLRVLQVDSLQYYYDMKRLHAF
                     HPDYLVGLAPNQDRTIRHYAGLWQGRFTTNSLGYRGSPEPDGSPVIACLGDSLVMGFG
                     VSDEETFCAKLAGINLHGKKHQAMNLGVDAFGSVGDYKRLKEASGQLNLDTVLLFVSP
                     NDFTIPPELAKRGVRPDDLIDEDRKNHPELTRFFRIQFEATRISYLLHALKLSREQLA
                     VTMVTRRESVRSQMVQAGLSQPEEGDRISSSTGRYILEQFYAFKKHATCEPEKTSNAQ
                     ASCPEPVPDQAGYACIPRPAASELEPLPAITMEAYRNMISLAREKNFRLVPVFLPVQV
                     EELYCDMHGKHHALGNYAVRAQAFFEKEGIRTIDLADKVRDICKLEKHSVLDQIIPGD
                     GHLTKSGNIWAARALREKLNAL"
     gene            2959..4386
                     /locus_tag="PKK76_09580"
     CDS             2959..4386
                     /locus_tag="PKK76_09580"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNL68765.1"
                     /translation="MLFNSVHFLIFAPVVMAVTFLLPQRLQRWWLLLASLYFYAVANV
                     VFLIPLLFCIGITYVLVRWMVSVESQVQKRIALLGVIFGNLGILYSLKYMDLSFWVWN
                     HTLGLRPCDPWYAHSLGMILPMGISFFTLMAIGYGVDVYRGFEGRRSFLDTALFLSVF
                     FHLVAGPILRGRDLIDQFAQKIGWSWENFELGMRQICLGIVKKTFLADNISPTVDAIF
                     AAPAQMHWVSLLLSVFLHSFQIYCDFSGYSDIAIGSARILGFHIPENFNRPFLSLTMT
                     EMWRRWHISFSSWVRDYVYFPLGGSRTSPPRAYFNLFMTMFVSGIWHGPTMNFVLWGV
                     MHAVWLVLEKLLFSVDRIKKAYDSIPSILKIAYTFTAFSLALFFFRARMLPGFSSEME
                     VGFFMLKRAFTLVPGELVLPPAGVAVGILILWILEAIVEKNEHVFAPLWERKGVCAAG
                     AVALLFISFCIYSVTVSAQFIYFQF"
     regulatory      4387..4515
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4640..>5799
                     /locus_tag="PKK76_09585"
     CDS             4640..>5799
                     /locus_tag="PKK76_09585"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNL68766.1"
                     /translation="MFAQEIEVTGKKRTLGDQPAATSEIPVSDYAGRFETLEDILEKE
                     TSVRVKRYGGPGSQSSISVRGSNANQTNLYLDGIPLNNAVSGEANLADYNLDGVGSIQ
                     VFRSGQSSGFSGSAIGGSVNLVPERGESGTRLRLRGGTERTIDVAARAGGSRNPEEAE
                     KEETEKAKVDPSLLDAMRAQWSVSGHTGRSDQTYKYRNNNGTPVFNTFDDHDDRRKNA
                     WYRDDGFTGYASAGVGNTDLKILDDFTYRMHGVPGPGSRQTEKTHRELTRNTTGIGSD
                     TRGLGVDWIRLETRAYYTYYRESFFDPLQEFSTSSPNSSGKLDVYGIHLMPSLYFPVA
                     FQTIRFLFAEEREDYHQEIRNRLDQKTENVPARFRRHTSLHAEDEFSFFKKRL"
BASE COUNT         1298 a         1428 c         1508 g         1565 t
ORIGIN      
        1 gttgcgctcg agcgcgggcc tgaaccttgg aattgatttt ttgcccggag cttttccttt
       61 cgcgccacca attgcccgtg cgcttgcggc agacatcgtc tggtttgatt ctctggtcat
      121 gaatgtagat cgcacggcca aaaaccccaa tctacttcgc tggcacaatc aagtttggat
      181 catagatcat ggagcgtgtc tgccggccca gcacggcgat gatcctttat cggatgcggc
      241 acttcgagaa tttccacgca tcgcggatca tgttcttttg aaacacgcag gctccattgc
      301 agacgcacac agtcgcctcg cgcctctttt gagcgcagag atgattcggg atgcagcggc
      361 acttgtgccg gattcgtggt tcatcaaaca cgggcgggaa gaatacatag acttcgtaca
      421 gaaacgtttg agttccgggc catttgcaga ggaggctgag cgtgtccgat cgtgaagcat
      481 accagtacgc catgtggcgc gtcgtccctt tgctcgaaag aggcgaattc atcaatgcag
      541 gtgtagtttt gttttcacgc aggcatagat tcctcaaggc gcaagttcat ctagatgaag
      601 tgcgtctcaa ggcaatctgt ccggatgtgg atctggagaa cgttcgttcg cagcttacgt
      661 ttcgcgaaaa gatcgcagcc ggtgaccctg agggtggatc cgttgctctt caatcacaat
      721 ccgagcggtt tggctggctt gtggctcctg caagcacagt catccaacct tcgcaggtcc
      781 acaccggcct ttgcgacgat ccggaggcgg cgctctcgca tttgttcaaa aggctggtaa
      841 tgatcgccta acgtggcggg gcgttcatca ggctcatcgc ggatgcgaag tacctgtctt
      901 tcattctgtc caccagatcg ggtagattct tgagttcggc ttctagatgt tttttgcgga
      961 atcgatcgag agttttgaga gtgaccacac aatcatgcca ctttccaagc aagtctgctg
     1021 ttttttcgaa tggaacaaga ggggtgctgc cgggcggtag aagcatactg agaatggccg
     1081 cttcctttac cttcgcccgt aatgcgtgaa tttgttccgc attcattgtt tgcgatgcag
     1141 actcatttac atccgttatt cgcccttcga tgttcttgcg aatcgattct tcgagcccgg
     1201 tgtcaggttc ctggtttgta tgtctttgaa tcgcgcggac taaatcctca agtgtatctg
     1261 ctggaatcat ccgtgtgatg gtgcgaagac ggcgcctcgc ttttttccgt ctgcgtttga
     1321 gaaattgctt cagattgtct ggcatccgcc ggccatcgag tgtttcctcg aggatagaac
     1381 gctgcacatc tatgtttctg attctaccgg cggcgcgaaa ggcaggcgaa agcaaaacaa
     1441 gagagggtgg agatggaagg gaagggagca atgcccgaag tcgtttcacg gccagcctga
     1501 attcgtgcag attctctttc tgtcctttcc tgtgaagatc gtagaaggta gattcaatga
     1561 cgcgcgcgca tttatgaatg gtcttgcgat aggctgcggc ggcggtcatt cttcaagttg
     1621 cccctgtcgg gcgttttctg gccatgagaa aacgagtaaa acgtgatgga ttgaggtcgc
     1681 atggttttgt ttacatcctg cttcgatttt ggaattctgc ctccatgctc cggtcaaggc
     1741 tggtgtccgg ttttctcatc gtcgtgggag ctctacttgc cgcggagatt ggcctgcgcg
     1801 tcctgcaggt ggattctctt caatattatt acgacatgaa gcgtttgcac gctttccatc
     1861 cagattatct ggttggactc gctcccaatc aggatcgcac cattcgtcac tatgcaggtc
     1921 tctggcaggg tcgcttcact accaattcac ttggttatcg cggtagcccg gagccggacg
     1981 ggagtccagt catcgcctgc ctcggagata gcctggtgat gggatttggc gtaagcgacg
     2041 aagaaacctt ttgcgcgaag ttagccggca taaaccttca cggaaaaaag caccaggcaa
     2101 tgaacctggg tgtggacgcg tttggctctg tgggagacta caaacgactc aaagaagcat
     2161 ccggccaatt gaatctggat actgtgctct tgtttgtttc tccaaacgat tttacaattc
     2221 cgcctgagct tgcgaagcgt ggagtgagac ctgacgatct catagacgaa gacagaaaga
     2281 atcatccgga gttgacccga ttttttcgca ttcaatttga ggcaacgcgc atttcttatt
     2341 tgcttcatgc actcaaatta tcgcgagagc aacttgccgt aaccatggtt acgcgacgag
     2401 aatctgttcg ctctcaaatg gttcaggctg gcctttcgca gccggaggaa ggggacagga
     2461 ttagttcgag caccgggcgc tatatcctgg aacaattcta cgcctttaag aaacacgcaa
     2521 cctgtgagcc agaaaaaact tcgaacgcac aagcatcctg cccggaacct gtgccggatc
     2581 aggcgggtta cgcgtgcatt cccaggcctg ccgcgtcaga actggagcct ctccctgcca
     2641 tcaccatgga agcatatcga aacatgattt ccctcgcgcg agaaaagaat tttcgcctgg
     2701 ttcctgtctt tctccccgtt caggtcgaag agctctactg cgacatgcat ggcaagcacc
     2761 atgcactcgg aaactatgca gtgcgtgccc aggcattctt tgagaaagaa ggcattcgca
     2821 cgattgatct ggcggacaag gttcgggata tttgcaaact ggaaaaacat tctgtactgg
     2881 atcagatcat cccgggagac gggcacctga caaaatcagg gaacatctgg gctgcccggg
     2941 cattgagaga gaagctgaat gctctttaat tcagttcatt tcctcatatt cgcgccagta
     3001 gtcatggcag ttacgttttt gcttcctcag agattgcaga ggtggtggct ccttcttgca
     3061 agtctctatt tctatgccgt tgccaacgta gtttttctca ttcctcttct attctgcatt
     3121 ggaatcacct acgtactcgt tcgctggatg gtttcggtgg aaagtcaggt gcaaaaaaga
     3181 atcgcactcc tcggtgtgat ttttggaaac ctcgggattc tatattctct caagtatatg
     3241 gatctttctt tctgggtttg gaatcacaca ctgggtcttc gtccctgcga tccatggtat
     3301 gcgcattccc tgggaatgat ccttcccatg ggcatttcat ttttcactct catggccatt
     3361 ggttatggcg tggacgtata tcgaggattt gaaggaagac gcagctttct ggatacggca
     3421 ctcttcttat ctgtgttctt tcatctggtg gccggaccaa ttttacgcgg acgagatttg
     3481 atcgatcaat tcgcgcagaa gataggatgg agctgggaaa attttgagct cggaatgcgg
     3541 cagatctgtc tcgggatcgt gaagaagaca ttccttgcag ataatatcag tccaacggta
     3601 gatgcgattt ttgcagcacc cgcgcagatg cactgggttt cacttttgct ttctgtattt
     3661 ctgcattctt ttcagattta ctgcgatttt tccggctatt ctgatattgc aattggctcc
     3721 gcgcgcattc ttggatttca cattcctgaa aacttcaaca gaccttttct ttctctcaca
     3781 atgacagaga tgtggagaag atggcatatt tccttttcct cctgggtgcg cgattacgtg
     3841 tatttcccgc tcggaggttc gcgcacttca cctcctcgag catactttaa tttatttatg
     3901 actatgtttg tcagcggaat ctggcacggc cccaccatga actttgtgct ctggggtgtg
     3961 atgcacgcgg tatggctggt ccttgaaaag ctattgttct ctgtggatcg aatcaagaaa
     4021 gcgtatgatt ctatcccttc tatcctgaag attgcctata ctttcaccgc attttctctt
     4081 gctctcttct tctttcgcgc gcggatgctc cccggttttt cgagcgaaat ggaagtaggt
     4141 ttcttcatgc tcaagcgtgc tttcacactg gttccaggtg agctggtgct gcctccagct
     4201 ggagtggccg ttgggatctt gattctatgg attctagaag caatcgtgga aaaaaatgaa
     4261 catgtatttg ctccactgtg ggaacgtaaa ggtgtctgcg ctgctggagc agtggctttg
     4321 ctcttcatca gtttctgcat ctactctgtc actgtgagcg ctcagttcat ctactttcaa
     4381 ttttgattat cgcttgaccc tgaaggctcc ggtcgcccgc tgttttaatc tccgggagga
     4441 aaggggtgca attcccctgc tgacgcgcaa ctgtaagggc tttgcccgag ccagatcttc
     4501 tccgcagtac ggactcgcga atcggtccac tgctaactcg cagagcgagg agcccccgga
     4561 ttcgggatgc ttttggaggg actgtctgcg atttctctgt tatttctcaa tcgccctcgc
     4621 atttcttgcg cctggtctta tgttcgcgca agagatcgag gtaactggca aaaaaagaac
     4681 gttaggcgat cagccggctg cgacttcgga gatccctgta tctgattatg ccggcagatt
     4741 tgaaaccctg gaagatattc ttgaaaagga aacctccgtg cgcgtgaagc gatatggagg
     4801 tccgggctca cagagttcca tttcagttcg tggttctaac gcaaaccaga ccaacctgta
     4861 tctggatgga attccgttaa acaacgcggt gagcggggaa gccaatctcg ccgattataa
     4921 tctggacggc gtgggtagca tccaggtatt tcgctccggg caatcgtctg gtttcagtgg
     4981 ttctgccatt ggcggatccg tgaatctggt gcccgaaaga ggcgaaagtg gaacgaggtt
     5041 gcgccttcgc ggaggaaccg aacgcacgat tgatgttgcg gcgcgggcag gcggttctcg
     5101 caatccagaa gaggcggaaa aagaggaaac agaaaaggct aaagtggatc cttccttgct
     5161 tgatgcgatg cgagcgcagt ggtcggtgtc cggccacacg ggccgatcag accaaacata
     5221 caaatatcgc aacaacaacg gcactcccgt attcaacact tttgatgacc atgatgatag
     5281 aagaaagaac gcctggtaca gggacgacgg attcactggc tatgcaagcg ctggcgtggg
     5341 aaacacagat ttaaagattc tagacgattt tacatatcgc atgcacggag tcccgggtcc
     5401 cggcagtcgg cagacagaaa agacacaccg cgaattgaca cgcaacacga ctggaattgg
     5461 atcagatacg cgaggccttg gagtagactg gattcgtctg gagaccagag cgtattatac
     5521 gtactacaga gaatctttct tcgatcctct ccaggagttt tctacttcct cccccaattc
     5581 gagcggcaag ctcgatgtat atggaattca tttgatgcca tctctctatt ttccggtcgc
     5641 tttccagacc attcgctttc tatttgcgga agagagagaa gactatcatc aggagattcg
     5701 aaatcgcctg gaccagaaaa cggaaaatgt accggcccga tttcgcaggc acacttccct
     5761 tcacgcagaa gacgaatttt catttttcaa aaaaagact
//
LOCUS       DAORFF010000207         5783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7481_length_5783_cov_3.851432, whole genome shotgun sequence.
ACCESSION   DAORFF010000207 DAORFF010000000
VERSION     DAORFF010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7481_length_5783_cov_3.851432"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1238)
                     /locus_tag="PKK76_09590"
     CDS             complement(78..1238)
                     /locus_tag="PKK76_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNL68767.1"
                     /translation="MIASISGALRSHRFQVLLRYGMSGAVVFAFNMGLAAFLFSFQWT
                     SESDLHTNAANVLATEVSFIFSFFFHNFLTWRQRGPGIVRRFVHFHLVAAAGLAVRFI
                     LFALMIAFDASWLVSTLLSIGCQMFLNFVGFDRLVFSLAKQPLASAAEAYAADGAGVL
                     TLDTLEEAVNYNRYVLSKIAPSLGASNLELGAGRGTISALVAETHTVTLCELSQDNLR
                     ALRERFRGSSRVRAISEDFFRFKQREFDCIYSANVLEHIEDDERVLEHACELLTPGGR
                     FVAYVPAGMWLYSSFDRKIGHYRRYTRQDVRRLRSFVKERGLPLQLKWRYMNLPGALG
                     WFIRMRLLKASHIDRRSALVADRLVGLFAILDRLPLPLGQNMLLEFEYGRKQ"
     gene            complement(1235..3331)
                     /locus_tag="PKK76_09595"
     CDS             complement(1235..3331)
                     /locus_tag="PKK76_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68768.1"
                     /translation="MKKIGLILIAVYHGLLFVVCSMNLMANNFVLKASRFYDEQLVSS
                     DLMSRWILLAPIALPLWMRALRSAGVTGFRITPAFLILAFCSICLNYPFALTGAQGVD
                     FWDILRAAVRDGLTQPSAFCISVLSLVGLIEFRNVEEKRLQILLPAMGVAAVLFKFIL
                     AGAALPRLEDEVAYFIQSLIFESGSRHALLIPPTGMALESLKDAAFLPFLIQTGNEYY
                     SAHMHGWSIVLSLFSMVHAKSIATLVFTLLNIGLFYALSRHFLSEAVASARIIALSLF
                     VASPVLLFLSNTYMSHTLALALSQCVVLAHAKLTRLRPIWMVVFVLASVAAFFVRPQS
                     IVPLMAALVVYEMVRFACSEAKAASALRILFIVLSCGLAYGALSLYADWMRFDSVIPM
                     TAYVKQYLVDGCQSLGFGPGHGCFPTYGTFGHSFRKTIWNIGDLVARCSQELAVGGVP
                     LVLGVIVLIVRHFRQIRVDGPETLFLLILVITIGEFGLYFHNGGESYRGRYLAEVAFA
                     LMLLVGLLIRHETILTSRAASVAAMGIAFALPVISLFQIRGEYFHPVLGSFSSVGDRN
                     PSIRDSLIRVEPVQGGVETVRSEFGGPDETIELTRRKRYLNLGYGTLAATAVRVNANG
                     LLEDARGNVLLGDISDEDAQRFGRPVYLLRYSAYEPVTKSSKIVRIWKRSPAVVERRG
                     DSLLKSENSLKDRNGQ"
     gene            complement(3341..5380)
                     /locus_tag="PKK76_09600"
     CDS             complement(3341..5380)
                     /locus_tag="PKK76_09600"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNL68769.1"
                     /translation="MQKLFYPLLGLLFVLSTLLSLAFGSLDVSLLREGGAFLVMVVLP
                     FWVYHRRRFKTLADHVFFWASVLTITHSFLNGITYLADTPVPRLKLYLKTFFLASILL
                     WGIAWFRGASRSLLVALCILFSANVLLSWYFLGFTLRSTVLAAYLIGGLVYALEEVET
                     VRLDYRDAAMVLLFGAGLLSTIFSWDRSSAFFGYTHLGLALPLFFLGRHLSKTADVPE
                     FLRNCVFAFSGVMIMFAVFLLVTLYTSGQPLTDKMEKIAGYHVNGLGASIALFLPLCI
                     YVGVNDSNPWVRIWSRSCAIISAGMLLIISSRGSMVGVIISLLLLVIQVRRVRLSGWS
                     ARAIVILLIGAGAVVLYFVEPLRQYVQALGSPESMKIRLMIWRLHIANVLHYSPWIGF
                     GPDTFIINAVIPPADPELSGALKPFMRGFGPIVHAHNVFVQLLQDFGLLGILFSVILG
                     VSVAGVAWRFLFGADAPGLRSYLPYGFAALTALFVQGLFDYTLSDPMTYYPAVMILGM
                     ILQVAPGKHALEMSLHKITSIVFGAGIVLFVVLFWMGWNRTLYISETSVFRGQTLSDR
                     FGNVTFLGISVFTDEQIRAFQFRDRLLLPGNTDHRIEQLAGEFYFQRFKRQGYTDGLT
                     VARSRFERCLAIHPYSALCAWRLSTIHHELGSKTEEERYLDLASIHDPYKLVIDQ"
BASE COUNT         1774 a         1525 c         1375 g         1109 t
ORIGIN      
        1 tcagaagctt gtctattacg aggccaatcg ttgcttcctc attgaatgcc gggaccagca
       61 cactgaccgg ggccctgcta ttgcttgcgg ccatattcaa actccagaag catattttgc
      121 cccagcggca aaggtagtcg atcaagaatc gcgaagagtc ccacaagcct gtcagccaca
      181 agagcactcc gccgatcaat gtgcgaagct ttcaataaac gcattctgat aaaccagcca
      241 agagcaccgg gtaaattcat atagcgccac ttcaattgaa gcggcaaacc gcgttctttc
      301 acgaacgagc gaaggcgtcg cacgtcctgc cgcgtgtatc tgcggtagtg accaatcttg
      361 cgatcaaaag aagaataaag ccacatcccc gcggggacgt acgcaacaaa ccgtcctccg
      421 ggagtgagca actcgcaggc atgttcgagc actcgctcgt cgtcttcaat gtgttccagt
      481 acgttggcag aataaatgca atcaaattct ctctgcttga accgaaagaa atcttccgaa
      541 atcgcgcgaa cacgcgagga tccgcgaaac ctttcgcgca gtgcgcgcaa attgtcctgc
      601 gaaagctcac agagagtaac cgtatgcgtc tctgcaacta gagccgatat ggttcccctg
      661 cccgcaccaa gctcaagatt ggaagcaccc agtgaaggcg cgatcttact cagtacatat
      721 cgattgtaat ttacagcttc ttcgagggta tccagagtca gcacgcccgc tccgtccgcc
      781 gcataggcct cagcggctga ggccagaggt tgttttgcaa gcgaaaacac aaggcgatca
      841 aacccaacaa aattcaagaa catctggcag ccaatagaaa gaagcgtact taccaaccag
      901 gacgcatcaa atgcgatcat taatgcgaac aaaatgaaac gtacggccag tcccgccgca
      961 gccaccagat gaaaatgaac aaagcgccga acaatcccag gccctcgctg cctccaggtc
     1021 aaaaagttat ggaagaagaa tgaaaatatg aaagatactt ctgtggccag aacgttagcc
     1081 gcattcgtgt gcaaatccga ttcagacgtc cactgaaaag aaaaaagaaa cgcggccagg
     1141 cccatgttga atgcaaagac cactgcaccg ctcatgccat agcgaaggag cacctgaaag
     1201 cgatgagagc gcaaggcacc ggaaatactg gcaatcattg cccgttccga tcctttaatg
     1261 aattctccga cttaagaagc gaatctcctc tgcgctccac aactgcgggc gaccttttcc
     1321 atattcgcac aatcttgctg cttttagtca caggttcata ggcagaatat cgcagcagat
     1381 acaccggcct tccgaatcgc tgtgcgtctt catctgaaat gtctccgagc aatacattgc
     1441 cgcgggcatc ttccaggagc ccatttgcat tcacgcgaac ggcagtagcg gccagtgttc
     1501 cataacccag gttcaagtat cgtttccttc tagtaagttc aatggtttca tccgggccgc
     1561 caaattcaga ccgaactgtc tccactcctc cttgcactgg ttcaactcga atcagagagt
     1621 cgcgaatcga gggatttcta tccccaaccg atgagaaaga acccaggact ggatgaaaat
     1681 actcccctcg aatctggaat aaggaaatca cgggcaatgc aaaggcgatt cccattgcag
     1741 cgacgctcgc tgcgcgcgaa gtgagaatcg tctcatggcg aatcaggaga ccaaccagta
     1801 gcattagtgc aaaagcaact tcggccaggt atctgccgcg atagctttcg ccgccgttat
     1861 gaaagtaaag tccaaactca ccgatcgtaa tgacaagtat caacagaaac aatgtttcgg
     1921 gtccgtccac gcggatctgg cgaaagtggc gcacgatcag tacaatcacg cctaacacca
     1981 gtggaacacc accaaccgcg agctcctggc tgcacctggc caccaaatca cctatgttcc
     2041 agattgtttt gcgaaatgag tgaccaaacg ttccgtacgt tgggaagcat ccatgcccgg
     2101 ggccaaatcc gagagattga caaccgtcca ctagatattg cttcacatac gctgtcatcg
     2161 gaatcacgga atcaaatcgc atccaatccg cataaagaga caacgcgcca tatgccagac
     2221 cgcatgacaa aacaatgaac agaatgcgca aagcagacgc cgccttcgcc tcgctacatg
     2281 caaagcgaac catttcataa actacgaggg cagccatgag cgggacaatg gattgcggtc
     2341 ttacaaaaaa tgcagctaca gacgccaaaa caaaaaccac catccagatg ggccttaagc
     2401 gagttagctt cgcatgcgca agcacaacac actgcgaaag agcaagagcc agagtgtgcg
     2461 acatgtatgt gttggagaga aacagcaaga ccggtgaagc aacgaaaagg gacagagcaa
     2521 tgattcgcgc agaagcaacc gcttctgaga gaaaatgacg agagagcgca taaaacaggc
     2581 caatgttcag aagtgtaaaa acaagagttg cgatggactt tgcgtgaacc atggaaaaca
     2641 gactgagcac gatagaccaa ccgtgcatgt gtgcactgta gtattcgttt ccagtttgga
     2701 tcaagaaagg aagaaacgcg gcgtccttga gagactcgag ggccattccc gttggaggga
     2761 tcagaagtgc atgccttgaa ccgctttcga aaattagcga ttgaataaaa tacgcgactt
     2821 catcttcgag tcttggtaaa gccgcccccg ccagaatgaa cttgaacaat acggcagcta
     2881 cgcccatcgc aggaagtaga atctgaagac gcttttcttc cacattcctg aattcgatga
     2941 gcccgaccag agaaagaact gaaatgcaaa aagcggaagg ttgcgtcagg ccatctcgca
     3001 cagcagcgcg aagtatatcc cagaaatcga cgccctgggc cccggtaaga gcaaatggat
     3061 agttcaagca aatggaacaa aaggcaagaa ttaagaaagc tggcgtgatt cgaaagccag
     3121 tcacccccgc cgatcgcaat gcacgcatcc agaggggaag tgcaattggc gccagtagaa
     3181 tccagcgcga catcaaatca ctggagacta gctgttcgtc gtaaaatcga ctggctttga
     3241 ggacgaagtt gttggccatg aggttcatgg aacaaacaac aaaaagcaaa ccgtgataca
     3301 ccgcgattag gatcagtcct attttcttca tcgcgtctgc ctactgatcg attacaagct
     3361 tgtaaggatc atgaatgctt gcaagatcca gataacgttc ctcttccgtt ttacttccca
     3421 gttcatggtg aatcgtggaa agtcgccatg cgcaaagcgc agagtaagga tgaatggcaa
     3481 ggcatcgctc aaagcgagaa cgcgcaacgg tcagaccatc cgtgtaacct tgtctcttga
     3541 accgctgaaa atagaattcg cctgccagct gttcgattct gtgatccgta tttccaggca
     3601 aaagcaaacg atcacgaaat tgaaaagcgc gtatctgttc gtccgtgaac acgctgatgc
     3661 ccaggaaagt cacgtttcca aatcgatcag atagcgtttg tccgcgaaac acgctggtct
     3721 cggaaatata tagagtacga ttccatccca tccagaacaa gaccacaaag agcacaatgc
     3781 cagcaccaaa cacgatagac gttatcttat gaagggacat ttccagagca tgcttgcccg
     3841 gagctacctg gagaatcatt ccaagaatca tgacggcagg ataataagtc atcggatcac
     3901 tgagagtgta atcgaacaat ccctgcacaa acaaagctgt gagagcagca aatccgtacg
     3961 gcagatagga gcgaaggcct ggcgcatccg cgccaaatag gaagcgccat gcgacaccgg
     4021 caacagagac gccaagaatc acagagaaca gaatccccag caaaccaaaa tcctgaagaa
     4081 gttgaacgaa tacattgtgc gcgtgcacaa tcggaccgaa ccctctcatg aaaggtttca
     4141 aggctccact caattcaggg tcagcgggtg ggatcaccgc attgatgatg aatgtgtcag
     4201 gtccaaaacc aatccacgga gagtagtgca aaacattcgc aatatgcaga cgccagatca
     4261 tgaggcgaat cttcatggac tcagggctac cgagggcttg cacatattgg cgcaacggct
     4321 cgacgaaata tagaacaaca gcaccagcac cgatcaagag aattacaatg gctcgagcag
     4381 accatcctga cagccgaact ctacgaacct gaatgaccag caaaagcaaa gagatgatga
     4441 ctcccaccat cgatccccgt gaggaaatga taagtagcat cccggccgat atgatcgcac
     4501 agctacgact ccagatacga acccatggat tcgaatcgtt cacgccgaca tagatacaaa
     4561 ggggaaggaa caacgcgatc gatgcgccca gtccattgac gtgatagccg gcaattttct
     4621 ccatcttgtc cgtaagaggc tggccactgg tgtacagcgt aacaagcaag aacacggcaa
     4681 acatgatcat cacgccactg aacgcaaata cgcaattgcg caggaattca gggacatctg
     4741 ccgttttaga taaatgccga ccgagaaaga agagtggcag cgcgaggccc aggtgagtgt
     4801 aaccaaaaaa cgcactgctc cgatcccacg aaaaaatagt agaaagcaag cccgcgccga
     4861 acagcaacac catggcggcg tcacggtaat ccaggcgcac cgtctcaact tcttccaatg
     4921 catacaccag gccaccgatc aaatatgcag ccagaaccgt gctgcggagt gtgaaaccca
     4981 ggaagtacca ggaaagaagt acgtttgcag aaaataaaat acaaagtgca acgagcaagc
     5041 ttcgagacgc tccgcgaaac caggcgatgc cccacaatag aatcgaagcc agaaagaaag
     5101 ttttcaaata aagcttcaac ctgggaacgg gagtgtccgc gagataggta atgccgttca
     5161 gaaacgaatg agtgattgtc aaaacagaag cccagaagaa cacgtggtct gcgagcgtct
     5221 tgaacctgcg acgatggtaa acccagaagg gcaacaccac catgaccaga aatgcacccc
     5281 cttcgcgcaa aagagaaacg tcgagagatc caaaagccag agacaagagc gtgctcaaaa
     5341 caaacaggag gccgagtagt ggataaaaca acttttgcat gcaagccggg ctaacgcgtg
     5401 gcgcttcttc gaaaaaataa gcccttctca ggctttgacc agcatccggt cacgaccttc
     5461 aaagtaaacc aagtttggat gcagactttc cggtggctct tcaagcacat cagactttga
     5521 agaaatgact tgagccaatt gaatcgctca ttgcctatga gtctcacgac atgacgcata
     5581 cggttcgaca agcgatccgg gtatgattcg gtaacctcaa aggtaggaga taacttcatg
     5641 aaaaaatacg tgctctctct ggccatcagc agcctctttt ccactgcagt gatggcatcg
     5701 gccaatctgt ataactcaga ttcccagagc tacagactgc tcctcgattc aagcgaatcc
     5761 tgtttttccg gcactcacac cag
//
LOCUS       DAORFF010000208         5758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7520_length_5758_cov_4.157987, whole genome shotgun sequence.
ACCESSION   DAORFF010000208 DAORFF010000000
VERSION     DAORFF010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7520_length_5758_cov_4.157987"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKK76_09605"
     CDS             <1..231
                     /locus_tag="PKK76_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNL68770.1"
                     /translation="EMPAGREDLVIVPSLAVNKEGCRLGRGGGYYDRARDQLVRAKIV
                     SVLPDALAGLDFPGESHDLKVTTVITESGIHE"
     gene            224..718
                     /locus_tag="PKK76_09610"
     CDS             224..718
                     /locus_tag="PKK76_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNL68771.1"
                     /translation="MNKEIFSGHIEGLDLDSEKETELVQFLVFQCGDGIYGINILDTH
                     EILKPVPVTRLPNVDPEYLGVLNLRGNIIPLLDANRKFGDTYSEITNLTRIVVCASAG
                     KFLGILVDRILEVARLPSDRIEGREVSGLSHQYVSAVGRSDARLFLILNLQILSGKAE
                     EENG"
     gene            711..3776
                     /locus_tag="PKK76_09615"
     CDS             711..3776
                     /locus_tag="PKK76_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNL68772.1"
                     /translation="MAEDRYLELFLEEAGEQIEELSQALLRLEKEQTNLEVINDVFRN
                     AHTLKSSAAFVGLDDLSLLAHHMEDVLHMIRSGALQVTTPMVSLLFRCLDRIKSAVAV
                     VHGGGRPDDRFADLIQALDEVKPAGGAKAPATSPSQASASVTSSASPSQSADIPSSVL
                     SGAVAAPYSSGVPSAPYSAESSDSFELSFGEQEMLKSQAGTSRIYEGLVRLDPESPMK
                     GLRFMLLSQNLKKAGSIFKCSPSEAELDSGSSSNTFRFFFYGDISSAELTKLCQVDMV
                     EEVRVSEKTFGKLDAGKPAQEETAVKTRNIKVSSEKIDYLLNSVGELVITNSGLQKTY
                     EDLQGEYRDSPLLAELKSRIDQAARIARDLQSGIMKTRMIPVGLVFHRFTRPVRDLAL
                     ELGKEVEFEFHGEETELDKNIIDGLNEPLLHIVRNSLDHGIETPEQRRAAGKPPVATL
                     GMHAYQSGNSIFLEVRDDGRGLNKEAILKRARENGLVTPEAVPSDDEIYNFIFQAGFS
                     TAEKVTDLSGRGVGMNVVKKMVQEFKGSITIRNEPGQGCAFVLQFPLTLAIVAAILVR
                     VADEQYAFPLSDVVETIKIDRREITTLQGRDIIHLRGDILPVFSLGRLMGMHGDSGNE
                     EFPVIIASVGGRKLAFLVEDMIGKKEIVIKSLEQNFRAVLGLIGACLMGDGSIVMVLD
                     VHGLMELAQKESSQDSSLRALDALAAMEDNAVVATRRYAEKIESMNKRIRVRAHREPG
                     QDRREESRDRHREEGKEDRPVVQQFVPQMEVRVEKKPESPTPPAPVAPTEDEFIDRPH
                     REALEILQKEREERTKRAEIHEPHARISLSEADFAKLTSVVNTGTIQAGMVLSQLLGT
                     EVEVSVPDFQAIDFDRLPDFLPPDPLVGVFLSTEDNFRSILLLVFDEETGYKAAADLM
                     GLPVAESRSISEEDLQSVMSELTNIVCASILNALANKTGMSIPPTVPGYLHGPGRALI
                     EQITKRDADVVRRKLLYFSTDFYRGEMELLGRIFFMPQENSLAQIVKKI"
     gene            3788..4798
                     /gene="cheB"
                     /locus_tag="PKK76_09620"
     CDS             3788..4798
                     /gene="cheB"
                     /locus_tag="PKK76_09620"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HNL68773.1"
                     /translation="MPFRVLIVDDSLIVRAAVGDIIGSIPDTEICGRAVNGAEAIEKI
                     ATLKPDLVILDIEMPVMDGMTVLRELKLRGMKIPVIMLSVLTQHGARTTFQALELGAL
                     DFIPKPSAQAGLQLSDIAGFLVEKVSGLVASRAEKAYAAVAPRPDRHSAEKLELLVIG
                     SSTGGPQALHRIFRDFTEPFPAPILIVQHMPPVFTAAFAERLGQVGAVEVLEAADGME
                     IKPNRAILAPGNRHMVLKGGKISLNDEPTVRSHRPSVDYTLNRVVDEYGMRAAALIMT
                     GMGQDGLEAVTRLYQAGGLTLAQDEASSVVFGMNRRAIEAGVIDRVLTLEESAAVLRS
                     FF"
     gene            4850..5209
                     /locus_tag="PKK76_09625"
     CDS             4850..5209
                     /locus_tag="PKK76_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL68774.1"
                     /translation="MARVLIVDDAKFMRTLVRDALTKAGHEIVGEAENGNQAVDLYKN
                     LKPDLVTMDITMREKDGLDAASEILKSDGRARIIMVTALGQEDLLARAIKMGVKDFVV
                     KPFPPERLQQAAAKALS"
     gene            5213..>5758
                     /locus_tag="PKK76_09630"
     CDS             5213..>5758
                     /locus_tag="PKK76_09630"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNL68775.1"
                     /translation="MVSVASPSEEVAGFLVSWVGPDGEEKGPLTLLWDLIESYRIDIF
                     DVSLFKITEDFMSYMKSAGLTLEQASSFTVMAARLLYYKSRALLPDPGFEEETEDRLP
                     PELIQQLLEYRKFQFASDKLRDLEEITSGMMRRRVTDEVTGEEYFEASLSDLVHAYTM
                     VLARIKQEHEEPALYHIRITEV"
BASE COUNT         1437 a         1405 c         1518 g         1398 t
ORIGIN      
        1 gagatgcctg cgggccggga agatctggta attgtgcctt cacttgccgt taataaagaa
       61 ggttgtcgtc tgggtcgcgg cggtgggtat tacgatcggg cccgggatca gctcgtgcga
      121 gcgaagattg tatctgtctt gccggatgct ctggcaggac tggactttcc aggagaatcg
      181 cacgatctga aagtgacgac cgtgatcacg gaatcaggaa tacatgaata aagagatctt
      241 tagcggccat atagagggac tcgatctgga ctctgagaaa gagacagagc tggttcaatt
      301 tctcgtattt caatgcggag atggcatcta tgggatcaat attctggaca cccatgagat
      361 cctcaagcca gtgcctgtga ctcgcttacc caacgtagat ccggaatacc tgggcgtgct
      421 gaatcttcgc ggcaacatca ttccgcttct agatgccaat cgcaagtttg gagatacata
      481 ctctgaaatt accaatctca cgcgaatcgt ggtctgcgcg agcgcaggca aattcctcgg
      541 aatcctggta gatagaatcc tggaagtggc tcgccttcct tccgacagaa tcgagggccg
      601 ggaagtcagc ggtctttcgc accagtacgt cagtgcagtg gggagatcgg atgcgagatt
      661 gtttttgatt ttgaatctcc agattctttc tggcaaggca gaagaagaaa atggctgaag
      721 atcgttattt agagctcttc ctcgaagaag caggggagca aatagaagaa ctcagtcagg
      781 cgcttcttcg tctggaaaaa gaacagacca atctcgaagt gatcaacgat gtctttagaa
      841 acgcgcacac tctgaaatct tctgccgcct ttgttggtct cgatgatctg tctttgcttg
      901 cgcatcatat ggaagatgtt cttcatatga ttcgatccgg cgctcttcag gtcacgactc
      961 ccatggtcag cctgttgttt cgctgcctcg acagaattaa atccgcggtg gctgtcgtgc
     1021 atggtggcgg tcgtccggat gatcgcttcg ccgatttgat tcaggcactc gatgaagtaa
     1081 aacctgcggg aggtgcgaaa gcacctgcaa cttcaccttc gcaggcctct gcgtccgtca
     1141 cttcgtccgc atcgccttcg caatctgcag acattccttc ttctgttctt tcgggtgcag
     1201 tggccgctcc ctattcttcc ggagttccat ctgctcctta ttccgcagag tcatctgact
     1261 cattcgaact gtcttttggc gaacaggaga tgttgaagtc gcaagcgggc acttcaagaa
     1321 tttacgaagg acttgtcagg ctcgatcctg aatcccctat gaagggattg cgattcatgc
     1381 ttctttcgca gaatctgaag aaagcaggat cgattttcaa atgctctcca tcagaagcag
     1441 agcttgattc tggaagctct tccaatacgt ttcgattctt tttctatggc gatatctctt
     1501 ctgcagagtt aaccaaactc tgtcaggtgg acatggtgga agaggtccgt gtatctgaga
     1561 aaacatttgg caagctcgat gccggtaagc cagcccagga agagacagcg gtaaaaacgc
     1621 gtaatatcaa agtctcttcg gaaaaaatag actatttgct caacagtgtt ggagaactcg
     1681 taattacgaa ttctggactg caaaagactt atgaagattt gcagggcgag tatcgcgatt
     1741 cgccacttct ggcagaactc aaaagtagga tcgaccaagc ggcgcgcatc gcccgagact
     1801 tacagtcagg catcatgaag accaggatga ttccggtcgg gcttgtgttt catcgcttca
     1861 ctcgtcctgt gagggacctg gctctcgaac ttggaaaaga agttgaattc gaatttcacg
     1921 gagaagaaac cgagctcgac aaaaacatca ttgatggcct gaacgaacct cttcttcaca
     1981 ttgtgcgaaa ttcgctggat catggaattg aaactcctga acaaagacgc gctgcaggga
     2041 agcctccggt cgcgactctg ggtatgcacg cgtatcagag cggaaacagt atttttctgg
     2101 aagtgcgcga tgatggacgc ggcctcaaca aggaagcaat cctcaaaagg gcgcgcgaga
     2161 atggactcgt gactccagag gcagtcccct ctgacgatga aatctataac tttattttcc
     2221 aggcgggatt ctctactgct gagaaagtaa cggacctttc cggtcgcggc gttggcatga
     2281 acgtggtcaa gaagatggtg caggaattca agggatccat cacgattcgc aatgaaccag
     2341 gccagggttg cgcctttgtg ctgcagttcc ctctcactct cgccatcgtc gcggcgattc
     2401 tggtgcgagt tgcagatgag cagtatgcat ttccactttc agatgtagtg gaaaccatca
     2461 agattgatcg gcgcgaaatc actacgctgc agggcagaga catcattcat ttgcgcggag
     2521 atattctgcc tgtgttttct ctcgggcgac tcatgggcat gcacggcgat tcgggaaatg
     2581 aagaatttcc agtgatcatc gcaagtgtgg gcggcagaaa gctcgcattc ctcgtagagg
     2641 atatgattgg aaaaaaagaa atcgtgatca aatctctgga acagaatttt cgcgcagtgc
     2701 ttggactgat tggtgcctgt ctcatgggcg acggaagcat tgtcatggtt ctagatgtcc
     2761 atggacttat ggagcttgcg cagaaggaat cttctcagga ttctagtctg cgcgccctgg
     2821 atgcactggc tgccatggaa gacaatgcgg tcgttgcgac gcgccgttac gcagaaaaaa
     2881 tcgagagcat gaacaagaga attcgagtgc gagctcatcg tgagccgggc caggaccgtc
     2941 gcgaagaatc gcgcgaccgg cacagagagg aaggcaaaga agaccgtcct gtagtgcagc
     3001 aatttgttcc gcagatggaa gttcgcgttg agaaaaaacc agaatccccc actccacctg
     3061 ctcccgttgc cccgacagaa gatgaattca ttgataggcc acatcgcgaa gctctagaaa
     3121 ttttacagaa agagcgcgaa gagagaacga agcgtgctga gattcacgaa ccacatgcgc
     3181 gcatttcgct ttcggaagca gattttgcaa agctaacctc cgtggtgaat accggaacca
     3241 tccaggccgg aatggtcttg tcccagcttc tgggaacgga agtggaagtg tccgtgcctg
     3301 attttcaggc gatcgacttt gatcgacttc cggattttct tcctccagat ccgctcgtgg
     3361 gcgtatttct ttccacagag gacaatttcc gttccatcct tcttctggtg tttgatgaag
     3421 aaactggata caaagcagcc gcagacctca tgggtctgcc tgttgccgaa agtcgcagca
     3481 tttcagagga agatcttcag tctgtcatgt ctgaattgac caacattgta tgcgcgagca
     3541 ttctcaatgc tctggcaaat aagactggaa tgtctattcc tccgactgtc cccggctatc
     3601 tacacggacc gggtcgcgct ctcattgaac aaatcacaaa gcgcgatgca gatgtggttc
     3661 gtcgtaagct tctctatttc agcactgact tttatcgcgg tgaaatggag cttctgggcc
     3721 ggatcttctt tatgccgcaa gaaaattccc ttgcgcaaat agttaagaaa atttagtagt
     3781 tacccctatg ccatttcgcg ttctgattgt agacgattca ctgattgtgc gcgcggctgt
     3841 tggcgatatc attggaagta ttccagacac cgagatctgt ggacgcgcgg tcaacggcgc
     3901 agaagctatc gagaaaattg caactctcaa gccggatctg gtgattctgg atattgaaat
     3961 gcctgtgatg gacggcatga ctgtcctgcg agaactcaaa ttgcgcggca tgaaaattcc
     4021 ggtaatcatg ctttccgttc taactcaaca tggtgcgcgc acaacatttc aggcactgga
     4081 gctgggcgcg ctggatttta ttcccaagcc ttccgcgcag gcaggactgc agctttccga
     4141 tatcgctgga ttcctggtcg agaaagtcag tggcctggtt gcgtcgcgtg cggagaaagc
     4201 atatgccgca gttgcacctc gaccggacag gcactcagct gaaaaactcg agctcctggt
     4261 gattggctct tctacgggcg ggccgcaagc tctgcacaga attttcagag atttcacaga
     4321 accatttcca gcgcccattt tgatcgttca gcatatgccg ccggtattta cagcagcctt
     4381 cgcagaaagg ctcgggcagg tgggtgcagt agaagtactc gaggccgccg acgggatgga
     4441 aatcaaaccc aatcgtgcga ttctggctcc gggcaatcgg cacatggttc tcaaaggcgg
     4501 gaagatttcc ttaaacgatg aaccaacggt tcgatctcac agaccgtccg tggattacac
     4561 tctaaatcga gtggtggacg aatacggaat gcgagcggca gccctgatta tgactggaat
     4621 ggggcaggat ggactggaag cagtgacacg gctctaccag gcaggcgggc ttacacttgc
     4681 tcaggatgag gcttctagcg ttgtttttgg catgaatcgc cgggcgatcg aagctggagt
     4741 cattgaccgg gtgcttaccc tggaagaatc agccgccgtg ctgcgcagtt ttttttagat
     4801 tgcactgatg catgatctca aaaatcattc ctgtgggttt cgataaaaga tggctagagt
     4861 actgattgta gacgacgcaa agtttatgag gacgctcgta cgcgacgctc tcaccaaggc
     4921 aggccacgaa attgtgggcg aagctgaaaa cggcaaccag gcagtagacc tttacaaaaa
     4981 tctgaaaccg gacctggtca caatggacat caccatgcgt gaaaaagatg gtctggacgc
     5041 tgcatcagag attctcaagt cagatggccg cgctcgaatc attatggtaa ctgcactggg
     5101 ccaggaagac ctgctcgccc gcgcgatcaa gatgggcgtg aaagattttg tagtaaaacc
     5161 atttccgcct gagcgccttc agcaagccgc cgcgaaagca ctttcctgag tcatggtttc
     5221 cgttgccagc ccatccgagg aagtcgctgg ctttctagtt tcctgggtcg gaccagacgg
     5281 agaggaaaag ggccctctca ctctactctg ggatctaatt gaatcttacc gcatcgacat
     5341 cttcgatgta tctcttttca aaatcaccga agacttcatg tcctatatga aatccgcagg
     5401 gctcactctc gaacaggcga gttcattcac agtgatggcc gcgcgccttc tctattacaa
     5461 aagtcgcgca ctgttgccgg atcctggatt cgaagaagaa acagaagaca gacttcctcc
     5521 tgaactcatc cagcagctcc ttgaatacag aaagtttcaa tttgcatctg acaagcttcg
     5581 cgatttagaa gaaatcacat ccgggatgat gcgtcgccgc gtaacggatg aagttacagg
     5641 cgaagaatac tttgaggcca gcctttcgga tctcgttcac gcatacacga tggtccttgc
     5701 gcgaatcaag caggagcatg aagagcccgc actctatcat atccgaatca ctgaagta
//
LOCUS       DAORFF010000209         5748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7557_length_5748_cov_3.568242, whole genome shotgun sequence.
ACCESSION   DAORFF010000209 DAORFF010000000
VERSION     DAORFF010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7557_length_5748_cov_3.568242"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..704
                     /locus_tag="PKK76_09635"
     CDS             51..704
                     /locus_tag="PKK76_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNL68776.1"
                     /translation="MRRFAVIFDMDGLMIDSERLYIETETEMARKRGAILTRETIARM
                     MGRKPIESMRIYKEDLGLVDAPADLLHERDVLMMQRMEKDLTAMPGLFECLESFYGRY
                     ALAIATGAESKFLALAVDTLGIRNRFDVLVPSDEILEGKPSPEIYLRAMHELKIQPDH
                     CAVLEDSANGCLSARRAGAYVVAVPSEHTAHQDFTPAHFMADDLLHARDHILGVFGG"
     gene            701..2794
                     /locus_tag="PKK76_09640"
     CDS             701..2794
                     /locus_tag="PKK76_09640"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNL68777.1"
                     /translation="MNLKGKTALVAGGAGFIGSHAARRLHELGLRVRILDAGPKPSRG
                     FDEFEYVQGSVTDRALVDQVMKGVDLVVNAAALVQEGGSFELFRRVNVEGAVRLAESA
                     RRSGIKLFVQFSSVMVYGFDYPAMVRETGPFGGNENPYCQTKIEGELALLQMHRPGTF
                     DICIVRPGDVYGPGSVPWVIRPFRLMKSYQFMLVDGGNGTMNHVYIDNLIDAVILLVE
                     KNATGRAYNVTDGGRTTFREYFQRLAREAGRGRLPSLPYNVARAVAWAAALPYKLFGR
                     RPEVSADSIPFVTRPHPVSIEAITALGFVPRVTLDEGIERTMEWLRSVNYEELHPFRP
                     QTEEKPSRYRYAALIGVILLAYLLFFPVKIDPGPYVAQADVGYTGPFAPNQKLADIKM
                     LPISAHGPEHVVVDSGWIYAAVDSGAILRMRADGSSLEEFVQTGGRPLGFAFDAKKNL
                     IIADAVKGLLLVTAKKEIHVITDQVDGKPLQFTDAVVVSKSGKIYFTDASMRFSARGG
                     TFEASILDTLEHSCTGRLIEVEAGRAKVFSSGICFANGLALDSKEESVFVAETGEMRI
                     LRYWISGARKGVKEVFLDKLPGYPDNLMRGSSGKIWVGLPRPRSKFADLAAAHPILRK
                     ITLRLPRFLWPVPKPYGHVFAFDESGRIVADLQDPAGQYPDTTGVTETADRLYIQSLH
                     AEGLGYVLAAGRTAQ"
     gene            2804..3817
                     /locus_tag="PKK76_09645"
     CDS             2804..3817
                     /locus_tag="PKK76_09645"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL68778.1"
                     /translation="MNSTVLSPDLEIQTHPDYNTSHHTENGFRNPWKMEEPKPGPLDA
                     LGWMVSFAFKKREDLPLFSVPMDPSFVANHPDLRSAHWIGHSTVLLKLGGKYVLLDPV
                     FANRASPVPFAGPRREVDLPIEIEDLPGVDVVVLSHNHYDHLDTDAIARLAGKFDPLF
                     IVPLRNGDVMRSAGARRVIELDWRQSVSAEGMTFHCLPARHFSNRGLTDRNEMLWASF
                     LIEHDGWKAYFAGDTGYAEHFSEIGRMFPGIDLALIPIGAYKPRWFMREVHVDPEEAM
                     QAFVDLGASEFFAIHWGTFILAEEALHEPAERMEQLILKKKMDRSRFHILMAGQSVGL
                     DRF"
     gene            3861..4322
                     /locus_tag="PKK76_09650"
     CDS             3861..4322
                     /locus_tag="PKK76_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNL68779.1"
                     /translation="MDHRFDVETFWEFSGGEGMNFSRDHVIRFPGKTEIHGSSAPAFA
                     GNAANLNPEELFVSAISSCQMLTYLFLCFKNKLQIQSYEDHAEGELVRESAGKFWMKT
                     VTLKPIVKFRGADTQQVRALAIRLIHEAHVNCFIAQSVKTTVNIEPSLLFM"
     gene            4336..5469
                     /locus_tag="PKK76_09655"
     CDS             4336..5469
                     /locus_tag="PKK76_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68780.1"
                     /translation="MRTIDLIRKIYIDESVADSPLARNVRLRALPGVEIETTSQSAEI
                     LNHFRKNGNIEDKHTLLLYPFPGKFMSFCPGSDGMVCCNYFVINFGYGCLYDCHYCFL
                     QNYLNNPLLTMFANTNDLFAEIDKKIHGKKFHFRIGTGETTDSLALEPLTGMAEILVN
                     HFAELPNATLELKTKSSNVDSLLGLDHRGHTVISWSVNPPGIVSEVETGTATLEERLA
                     AARKAEAAGYRLAFHLDPVIHFEGWEKQYHDLIDLIFSTVRADSVAWLSLGSFRYLPG
                     LKTVIQGRFPEDTLTREEMVQGPDGKYRYFKTIREEMYRSIRAKVQSVDPKLFLYLCM
                     ETKRMWEDVFQFVPESAKNLDAGFEERRNYMDSLHPPAAAPVL"
     gene            5408..>5748
                     /locus_tag="PKK76_09660"
     CDS             5408..>5748
                     /locus_tag="PKK76_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68781.1"
                     /translation="MKKGAITWTVCILLLQPLFSETIPPEAAVENLTTNWKGDDTNPL
                     PSPDGSFLVFQSDRPGSLEDFNFWFSYNKNHSDRTGASSWTIPVPLRFPLTGEASDTM
                     KILRPQGSVGV"
BASE COUNT         1393 a         1378 c         1522 g         1455 t
ORIGIN      
        1 agtgattgca gaagcaggcg gcctggacga aatcactctc cccgcttaac atgcgccgtt
       61 ttgccgtcat tttcgatatg gacgggctga tgattgattc ggagcgcctt tacattgaaa
      121 cggaaacaga aatggcgcga aagagaggcg ccattcttac ccgcgaaacc atcgctcgaa
      181 tgatgggcag aaaaccaatc gagtccatga gaatctacaa agaggacctg gggcttgtgg
      241 atgcaccggc cgatctcctc catgaaagag atgttctgat gatgcagcgc atggagaagg
      301 atctgactgc catgccaggg ctctttgaat gcctcgaatc gttttacggg cgctatgcgc
      361 tggccattgc gacaggtgca gagtcaaaat ttctggcatt ggccgtggac actctgggga
      421 ttcgaaatcg ttttgatgta cttgttccat cggatgaaat actcgaaggc aaaccaagtc
      481 ctgaaattta tctgcgtgcc atgcatgagt tgaagatcca gccagatcat tgcgctgtgc
      541 ttgaggattc tgccaatgga tgcctttctg cgcgaagagc gggtgcgtat gttgtggccg
      601 tcccgtcaga acatactgct caccaggatt ttactccggc acattttatg gcagacgatc
      661 tattgcacgc ccgcgatcac atcctcggag tatttggcgg atgaatctca aaggaaaaac
      721 ggctctcgtc gcgggtggtg ctggtttcat cggatcccat gcggcgcgca gattgcatga
      781 actgggtctc agggttcgca ttctggacgc agggccaaag ccttcgcgag gattcgacga
      841 gtttgaatat gtgcagggta gtgtgacgga tcgcgcactc gtagatcagg tgatgaaagg
      901 cgtagatctg gttgtgaatg cggcggctct agtgcaagag ggtggatcgt ttgaattgtt
      961 tcgccgcgtg aatgtagagg gagcggttcg actggcggaa agtgcgcgaa gaagcggaat
     1021 caagctcttt gttcagtttt caagtgttat ggtctacggg tttgattatc cggccatggt
     1081 tcgtgaaact ggtccgtttg gcggaaatga aaatccatac tgtcaaacca agatcgaggg
     1141 agaacttgca cttctgcaga tgcacaggcc gggcacgttc gacatatgca ttgttcgccc
     1201 aggagatgtg tatggccctg gatctgttcc ctgggtgatt cgtccgtttc gcctgatgaa
     1261 atcttaccag tttatgcttg tggatggtgg gaacggcacc atgaatcacg tctacataga
     1321 caacctgata gacgcggtca ttttgcttgt ggaaaagaac gccacaggaa gggcgtataa
     1381 cgttacggac ggaggcagaa ccacgtttcg ggaatacttt caaagactgg cccgagaagc
     1441 aggtcgcgga cggcttccgt ctcttcctta caatgtcgct agagccgttg catgggcggc
     1501 cgctctcccg tacaaactgt ttgggcgcag gcccgaggtg tccgctgatt caattccctt
     1561 tgttacaagg ccccatcctg tctctataga agccattact gcacttggat ttgttccacg
     1621 cgtgaccctg gatgaaggaa tcgaaaggac catggaatgg cttagaagcg tgaactacga
     1681 agagctccat ccatttcgac cgcaaacaga ggagaaacct tctcgatacc gttatgcggc
     1741 actgatcggg gtgatccttc ttgcttactt gctttttttt ccagtcaaaa ttgatccagg
     1801 cccctatgta gcccaggcgg atgtggggta cactggaccc tttgcgccca accagaaact
     1861 ggcagatatc aaaatgctac ccatcagcgc tcatggcccc gaacatgtgg ttgtggactc
     1921 tggctggatt tatgccgcag tggattcagg tgcgatcctt cgcatgcggg cggatggatc
     1981 gagtctggaa gaatttgtac aaacaggagg acgtcctctt ggatttgcat tcgatgcaaa
     2041 gaagaacctg atcatcgcgg atgcagtgaa aggactgctc ctggtcactg cgaaaaaaga
     2101 aattcatgtg atcacagacc aggtggatgg aaagccgctt caatttacgg atgcagtggt
     2161 agtttcaaaa tcaggaaaaa tatacttcac agatgcaagc atgcgcttct ccgctcgcgg
     2221 cggaacgttt gaggcaagca ttctcgatac actggagcat tcctgtaccg gtcgtctcat
     2281 cgaagtggag gctggtcgcg cgaaggtctt ttcttctgga atttgttttg ccaatggtct
     2341 tgcgctcgat tcaaaagagg agagtgtatt tgtagcagaa acgggtgaga tgcgcatcct
     2401 tcgatactgg atttctggtg cgcgaaaagg tgtaaaagaa gtgttcctgg ataaacttcc
     2461 gggctacccg gacaatctaa tgcgaggcag ttccggtaag atctgggtgg gtcttccgag
     2521 gccgcgcagt aaatttgcag atcttgcggc tgctcatcca atcctgcgta agatcacact
     2581 gaggctccca cgttttctct ggcctgttcc aaaaccgtac ggtcatgtat tcgcatttga
     2641 tgaatccgga cgcattgtcg cggatcttca ggatcccgca ggccagtatc cagataccac
     2701 aggggtaaca gagacagcag atcgcctcta cattcagagt cttcatgccg aaggccttgg
     2761 atatgtgctc gccgcaggca ggactgcgca gtaatctgtt ggagtgaatt ccaccgttct
     2821 gtctcctgat cttgaaatcc aaacgcatcc tgactacaat acaagtcacc acactgaaaa
     2881 cgggtttcgc aatccgtgga aaatggaaga accaaagccg ggcccactcg atgctctggg
     2941 atggatggtt tctttcgcat tcaaaaagcg agaggatcta cctcttttct ctgttcccat
     3001 ggatccctcc tttgtagcca atcatccaga tctcagatct gctcactgga ttgggcattc
     3061 tactgtgttg ctcaagcttg gcggcaaata tgttctactc gatccagttt ttgcaaatcg
     3121 tgccagtcct gtcccatttg caggtcccag aagagaagtg gatttgccca ttgaaatcga
     3181 agacctcccc ggagtggatg ttgtggttct ttctcacaat cactacgatc acctggatac
     3241 ggatgcgatc gctcgtctgg cgggcaagtt tgatccttta ttcatagtgc cacttagaaa
     3301 tggagacgtt atgcgatcag caggagctcg tcgcgtgata gaactggact ggaggcagag
     3361 tgtcagtgcg gagggcatga cgtttcattg cttgccggcc agacattttt cgaacagagg
     3421 tcttacggat aggaatgaaa tgctgtgggc cagcttcctc attgagcacg atggatggaa
     3481 agcctacttt gcgggtgaca ccggttatgc ggaacatttc tccgaaatag gcagaatgtt
     3541 ccctggcatt gatctggctc tcattccaat cggcgcgtac aagccaagat ggtttatgcg
     3601 ggaggtgcat gtggatccag aggaagcaat gcaggccttt gtggatctgg gtgcgtctga
     3661 attttttgcc attcactggg gaacgtttat cctggcagag gaagctctgc atgagccagc
     3721 agagcgcatg gagcagctga ttttaaaaaa gaaaatggat cggtcgcgat ttcacatact
     3781 catggcaggt caatctgtcg gtctggatcg attctagtgc aaatcggttg cgctcgccta
     3841 acccggagat aaactgacct atggaccatc gctttgatgt agaaacattc tgggagttct
     3901 caggcggaga gggaatgaat ttcagccggg atcatgtgat ccgttttccc gggaaaacgg
     3961 aaattcacgg atcctccgcg cctgcatttg cgggaaacgc ggccaacctg aaccctgaag
     4021 agctatttgt ttctgccatc tcttcctgcc agatgctcac gtatctcttc ttgtgtttta
     4081 agaacaaact ccaaattcaa tcctacgaag atcatgccga gggagaactg gtgcgcgaat
     4141 ctgccggaaa attctggatg aagaccgtta cactcaagcc aatcgtgaaa tttcgtggcg
     4201 cggacaccca gcaggtgcgc gcacttgcca tcaggctcat tcatgaagcg catgtaaatt
     4261 gcttcatcgc gcaatctgtg aagacaaccg tgaacattga acccagtctt ttgtttatgt
     4321 gagcgccggt cgtttatgcg aaccattgat ctcattagga aaatttacat agatgaatcc
     4381 gttgcagatt ctcctctcgc gcgcaacgtg cgtcttcgcg ctcttccagg agtggaaatt
     4441 gaaacaacca gtcagtcagc ggagattcta aatcacttca gaaaaaacgg aaacatagaa
     4501 gacaaacaca ctctgttgct ctatcctttt ccaggtaagt tcatgtcctt ctgccctggc
     4561 tcggacggca tggtctgctg taactacttt gtgatcaatt ttggatatgg ctgtctctat
     4621 gactgccact attgttttct tcaaaattac ctgaacaatc ctcttttgac catgtttgca
     4681 aatacgaatg atttgtttgc ggaaattgat aaaaaaattc atggaaaaaa gtttcacttc
     4741 cgcattggca ccggagaaac cacggattct cttgcgctcg aaccgctcac agggatggcg
     4801 gaaatactgg tgaatcattt tgcggaactt ccgaacgcca ctctggaact aaaaacaaaa
     4861 tcctcaaacg tggatagctt gctcgggctc gatcatcgtg gccacacagt gatctcctgg
     4921 tctgtcaatc cgcctggaat cgtttcggaa gtggaaaccg ggacagccac cctggaagaa
     4981 cgtctggccg cggccaggaa ggcggaggct gccggatata gacttgcatt tcatctggat
     5041 cctgtgattc attttgaagg ctgggaaaaa cagtaccacg acctgatcga tctaatcttc
     5101 tccacagtga gggcagacag tgttgcctgg ctttccctgg gcagttttcg ctatttaccc
     5161 ggcctaaaga ccgtaattca gggacgtttt ccagaagaca cgctgactcg cgaggaaatg
     5221 gttcaaggac cggacggaaa gtaccgttat tttaagacga tacgagaaga aatgtaccga
     5281 tccatccgcg cgaaggtcca atctgtggac ccgaagcttt tcctgtatct ttgcatggaa
     5341 acgaagcgga tgtgggagga tgtgttccaa ttcgtgcccg aatctgcgaa gaacctggat
     5401 gctggatttg aagaaaggcg caattacatg gacagtctgc atcctcctgc tgcagcccct
     5461 gttctctgaa acgatcccgc cagaggcggc cgttgaaaat ctaactacca actggaaggg
     5521 agacgatacc aatccgcttc catcgccgga cggaagcttc cttgtgtttc agtccgatcg
     5581 tccgggctcc ctggaagatt tcaacttttg gttctcctat aataagaatc attcggatcg
     5641 cacgggcgct tcctcgtgga cgattcctgt gcctcttcgc tttcctctaa ccggcgaagc
     5701 gtctgatacg atgaagatcc ttcgtcctca gggaagtgtg ggagtaga
//
LOCUS       DAORFF010000210         5742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7578_length_5742_cov_3.308423, whole genome shotgun sequence.
ACCESSION   DAORFF010000210 DAORFF010000000
VERSION     DAORFF010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7578_length_5742_cov_3.308423"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..548)
                     /locus_tag="PKK76_09665"
     CDS             complement(90..548)
                     /locus_tag="PKK76_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68782.1"
                     /translation="MIRSLLFTILFLGSIPGFAQQKEEVYEGDIRESDLRRRELVEKV
                     KKGEIPAAIKLRFSRKESDFLVFYDIEGRSIYYRYREDRFDTDAEKRVADLIAGEAYE
                     VTGPFLGMYYASMMHSSDHPKYKEMLNNADAIPAFLFKSARPLRIEQILL"
     gene            complement(545..1681)
                     /locus_tag="PKK76_09670"
     CDS             complement(545..1681)
                     /locus_tag="PKK76_09670"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNL68783.1"
                     /translation="MWKTREWSPCSKEESWNSNPESAHNVSGTVRALTAFILVTASFL
                     ISSSIFAESGKEEKGPTRVEALRAKILYASSTERRNEIRKVKSLAADEQKLLIPDVVK
                     LCTEDLDPNVRETSIRILTEMKTQEAEKAFLTALKDKVDDVQLAAISGLREIRSKEAG
                     PPLVELLKSLDVKKEQTKYSSIMRALGALHHKTDSAPLIKTAEEADTHEEIRRSIILY
                     LGDVKAQDGYEFLIKLARNEEKEPDLRAYAVNSIGRMKEARAVPDLKQILETIRNQKS
                     PKERARMSRLRFQSMVALMRLGDRSMVPEVMSAARDDDPVVRLRAVRQLGEMRTPEAK
                     ELLEFKSKYDENARVKKAAKLALDEWDKTPGDTGSDLDDSDKEK"
     gene            complement(1608..2081)
                     /locus_tag="PKK76_09675"
     CDS             complement(1608..2081)
                     /locus_tag="PKK76_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL68784.1"
                     /translation="MDKGYILCVDDEVSVLETLREQLRLHFGQTHEVEVAASAEDALT
                     LIAQILESGGLIEVIITDQVMPGIKGDQFLEEVHKQLPDTIKILLTGHAGLASAIHAV
                     NKGGLNRYIEKPWNMDSLKEDISQLIHKFREHVENQRMVTMLERRVVELESRIGA"
     gene            2198..2437
                     /locus_tag="PKK76_09680"
     CDS             2198..2437
                     /locus_tag="PKK76_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68785.1"
                     /translation="MPRYLQSEQIIPNKGMSYTMYEIEDVNTILRMLTWIPDADQISI
                     YPKPPVKQLFAPERCAQIDESQFLGLWQEGENRKK"
     gene            complement(2440..2512)
                     /locus_tag="PKK76_09685"
     tRNA            complement(2440..2512)
                     /locus_tag="PKK76_09685"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2477..2479),aa:Phe,seq:gaa)
     gene            complement(2606..>3436)
                     /locus_tag="PKK76_09690"
     CDS             complement(2606..>3436)
                     /locus_tag="PKK76_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNL68786.1"
                     /translation="LNFVQKAVWFVESHFRETVTLEEIAQTCGVSAYHLTRTFATSMG
                     IPLMRYVRARRLSEAARQLIQTKANIVDVALETGYGSHEAFTRAFRDQFDLTPEQLRA
                     QGHANNITLVEALSMHSSTMTRIQPPRLESEGPRIIAGLIQRHDCSNPAGIPEQWQRF
                     SKWIGNIPGQIGDAAYGINYNFDEEGTFDYMCGVQVSGESTIPSDLKVLKLPAQTYAV
                     FEHAGHIAGIRGSFTSIWNWFSASDLKPTEAPNLEKYGRQFDPATGLGGLEIWIPIQK
                     "
     assembly_gap    3437..3536
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3678..4319)
                     /gene="lipB"
                     /locus_tag="PKK76_09695"
     CDS             complement(3678..4319)
                     /gene="lipB"
                     /locus_tag="PKK76_09695"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNL68787.1"
                     /translation="MTQRIKLITRIGKPLAYQEYLHLESDLRARREELILVCEHSPVI
                     TAGVQFKDKSLRVPQTELAPRGVAFYKTSRGGDVTAHEPGQIVIYVHVDLKARSLDVS
                     KFFKGLLESAQTAAKNSFGLDLDLNTEAPGLYVSGRKIASIGIQFKSFFTGHGLAINV
                     TNDLSTFSLIDPCGFDSLEMTSVEKEGGRVDKTGLFIDLFYEHLQKNHLPERA"
     gene            complement(4316..5176)
                     /locus_tag="PKK76_09700"
     CDS             complement(4316..5176)
                     /locus_tag="PKK76_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNL68788.1"
                     /translation="MIRIAAFGDIHGSFDEEDLRMIQHSDYDMVLIVGDLPGRTHAGT
                     LDVARRLARMPRPAYFMPGNHDGVSLIQLIAELQGNHALVERSHRRQFKLVENLRAAL
                     GPVSYCAYSLHEISIKGTAISLVSARPHSMGGTSFSYRPYLEKEFGISDMNQSAEKIK
                     RLLDEARSPVLVLAHNGPAGLGSKRDDIFGCDFRKEEGDFGDPDLADALDYAEGAKIQ
                     VLGVVAGHMHHRIRGGGERVTIAKRKNTLCVNCARVPRIFKEDGQTLRHHVRIEISEK
                     GMEVEQVTRP"
     gene            5284..5604
                     /locus_tag="PKK76_09705"
     CDS             5284..5604
                     /locus_tag="PKK76_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNL68789.1"
                     /translation="MNLKQIEQTLTQKRAELAGRADRLQQDITRQSSPLEEDWEEASI
                     QRENDQVLDALDDETRAELQQIDRALGRIREGKYGQCEKCSGPIAAERLEALPYATTC
                     VKCA"
BASE COUNT         1281 a         1417 c         1395 g         1549 t
ORIGIN      
        1 atgtcctcgc agcaactcat ctttgtttcg gaactcagcc acaaaccagg aagctggaaa
       61 aaagcgctgc tttcaagtac tatttgattc tataaaagga tctgttcgat tcgaaggggt
      121 cgtgcagact tgaataggaa agcggggatc gcatctgcgt tgttcagcat ttctttgtac
      181 tttggatgat ccgatgagtg catcatggag gcgtagtaca tgccaagaaa tgggcccgtg
      241 acctcgtagg cttctcctgc aatcagatct gccactctct tttccgcgtc cgtatcaaaa
      301 cgatcttcgc ggtagcgata gtagatggaa cgaccttcta tgtcgtagaa gaccaggaaa
      361 tccgattctt ttcttgagaa tcgaagcttg atggcggccg gtatttcacc cttcttgact
      421 ttttccacaa gttcgcgcct gcgcaaatcg gactcgcgaa tgtctccctc atatacttct
      481 tctttttgtt gagcgaaacc aggaatgctt cccagaaata gaatcgtgaa cagaagtgag
      541 cgaatcattt ttctttgtct gaatcgtcca ggtcggatcc tgtgtctcct ggagttttgt
      601 cccattcatc cagcgccagt tttgcggctt tcttcactcg cgcgttttca tcatatttgg
      661 atttgaattc gaggagttct tttgcttccg gagtgcgcat ctcaccgagc tgtctcacag
      721 cgcgaaggcg aaccaccgga tcatcatcgc gtgcggcgga catgacttct gggaccatgg
      781 acctgtctcc cagccgcatg agtgcaacca tgctctggaa acgaagacgg ctcatgcgcg
      841 ctctttcctt cgggcttttt tgattgcgaa tggtttcgag tatttgcttg agatccggaa
      901 ctgctcgcgc ttctttcatg cgcccgatgg aattgactgc atacgcccgg agatctggct
      961 ctttttcttc gtttcgcgcg agcttgatta agaactcgta accatcctgt gcctttacat
     1021 cccccagata cagtatgatg gatctgcgaa tttcttcgtg agtgtctgct tcttccgctg
     1081 tcttgatcaa aggagcagag tccgttttat gatgaagagc gcctaacgct cgcatgatgg
     1141 agctgtattt ggtttgttct ttcttaacgt ccagactctt cagcaattca accagaggtg
     1201 ggcctgcttc tttggagcga atttcgcgaa ggccgctgat ggcagccagc tgcacgtcat
     1261 ctactttgtc cttcagcgcc gtgaggaagg ctttttccgc ttcctgcgtt ttcatttctg
     1321 tcagaatgcg aatgctggtt tcgcgaacat ttggatctag atcttccgtg cacagtttga
     1381 ctacgtccgg gatcaacagt ttctgttcgt ccgcggccag gctcttgacc ttacggattt
     1441 catttctgcg ttcggtggaa cttgcgtaga gaattttcgc acgaagcgcc tctacgcgcg
     1501 tcggtccttt ttcttccttg cccgattctg caaatatgga agaagaaatg agaaaggatg
     1561 cggttacaag aatgaatgcg gtgagggccc ggacagttcc agaaacgtta tgcgccgatt
     1621 ctggattcga gttccacgac tcttctttcg agcatggtga ccattctctg gttttccaca
     1681 tgttctctga acttgtggat gagttgagat atgtcttctt taagagagtc catgttccac
     1741 ggtttttcaa tgtagcgatt gagcccgcct ttgttgactg catggatggc gctggccaga
     1801 cctgcgtggc ctgtgaggag gatcttgatt gtatccggta attgcttgtg cacttcttca
     1861 agaaactggt cccctttgat tccgggcatc acctggtctg tgatgatgac ctcaatgagg
     1921 cctccggatt ccaggatttg agcaatgaga gtgagtgcgt cttccgcgct cgctgcaact
     1981 tctacttcat gcgtttggcc gaaatggagt ctgagctgtt ctctgagagt ctcaagaaca
     2041 ctcacttcgt cgtcgacgca caatatatag cccttatcca cctgctcccc ctgaagatcg
     2101 acgcgtccgc gattttcttg agtaaacgcg aaggggaatt cctgtgcaag aaaaatattg
     2161 tactttgcag gtacttgctg tctaggccga aggaagtatg cctcgttatc tacagagtga
     2221 acaaatcatc ccaaacaaag gtatgagtta taccatgtat gagatagaag atgtaaacac
     2281 gattctcaga atgctcacct ggattccgga cgcggatcaa atttcaattt atcccaaacc
     2341 gcctgtgaag cagctttttg cgccggaacg ctgtgcacag attgatgaat ctcagtttct
     2401 aggattgtgg caggaaggcg aaaaccgcaa gaagtagatt gcccagggac ggaattgaac
     2461 cgccgacgca cggattttca gtccgacgct ctaccgactg agctacctgg gcgctctctg
     2521 gcagaacatc aaaatggccg ctctgtgtca aagtgttttc accagcggcc atgatttgag
     2581 aattctgcga aactgactct gtcgattact tctggatcgg aatccagatt tccagacctc
     2641 cgagtccggt tgcaggatca aattgtctgc cgtatttctc caagttgggt gcttccgttg
     2701 gcttcaggtc ggaagcggaa aaccaattcc atatggaagt gaaacttccg cgaataccgg
     2761 caatgtgtcc cgcatgctcg aacacggcat acgtctgcgc gggtagcttc aataccttca
     2821 aatccgacgg gatagttgat tcgccgctca cctgcactcc gcacatgtaa tcgaacgttc
     2881 cttcctcatc gaagttgtaa ttgatgccgt acgcagcatc tcctatctgc ccgggaatat
     2941 tcccaatcca cttgctgaag cgttgccatt gctccggaat tccggcagga ttggagcaat
     3001 cgtgtctctg aatcaatcct gcgatgatgc gagggccttc gctttccagg cgaggtggtt
     3061 ggattctggt catagttgaa ctgtgcatac tcaatgcctc cactagcgtg atgttgtttg
     3121 cgtgaccctg cgcacgaagt tgttcgggcg taagatcaaa ttgatcgcga aaggctcgag
     3181 tgaacgcttc gtgcgagccg taaccagttt cgagagcaac atccacgatg tttgcctttg
     3241 tctgtatgag ctgccttgcc gcttcactca gtcgccgtgc gcgaacatat cgcatcagag
     3301 gaattcccat ggatgtagca aaagttctag tcaggtgata cgcggaaaca ccgcaagttt
     3361 gggcgatttc ctccagggtt acggtttccc ggaagtgact ctccacgaac cagactgctt
     3421 tttgcacgaa attcaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggt
     3541 ttcccggaag tgactctcca cgaaccagac tgctttttgc acgaaattca attgaatgcc
     3601 tcttgatgct gatgaatcat aacacagccc ccgttcggtg ttcttgatcg ttcttgcgaa
     3661 ttattctttt ttttgaatca cgctcgttct ggaaggtgat tcttctgtaa gtgctcgtaa
     3721 aagagatcaa taaacagtcc ggtcttatct actctgccgc cttctttctc gacgctcgtc
     3781 atttcgaggg aatcaaaccc gcacggatca atcagagaaa aagtagaaag gtcattggtg
     3841 acgttgatcg ccagaccgtg tcccgtaaaa aacgatttga attgaattcc aatggatgca
     3901 atctttctgc cactcacgta tagtcctggt gcctccgtgt ttagatccag atccagtccg
     3961 aacgaatttt tggcggccgt ctgcgcactt tctagcaatc ctttaaagaa tttggaaacg
     4021 tccaggcttc ttgccttcag atccacgtga acgtaaatga cgatctgccc tggttcatgc
     4081 gccgtaacat ccccaccgcg ggaagttttg taaaaggcca ccccccgtgg cgcaagctca
     4141 gtttgcggca cacgaaggct tttgtctttg aattgaacgc cggctgtgat gactggactg
     4201 tgctcgcaga ctagaatcag ttcttccctt cgcgcgcgca gatcagattc cagatggaga
     4261 tattcctgat aggcaagcgg ttttccaatt cgcgtaatca gtttgatcct ttgggtcacg
     4321 gcctggtcac ctgttctact tccattccct tttcggaaat ttcaattcga acatgatggc
     4381 gaagcgtttg cccatcttct ttaaagattc tcggaacgcg cgcgcagttg acgcataacg
     4441 tgtttttccg cttagcaatg gttacgcgtt caccaccgcc gcgaattctg tggtgcatgt
     4501 ggcctgccac gacgccaagg acctggatct ttgctccttc cgcataatcc agagcatctg
     4561 caagatctgg atctccgaaa tcgccttctt ctttgcgaaa gtcgcaacca aaaatgtcat
     4621 ctctttttga accaaggcct gcgggtccgt tgtgtgcgag aaccagcacg ggggatctgg
     4681 cttcgtctaa caatcgtttg attttttcgg ctgactgatt catgtcagag atcccgaatt
     4741 ctttttctag atacggtctg taggaaaaag aggtgccgcc catgctgtgt ggtctggctg
     4801 atacgagaga gatggccgtt cctttgatgc tgatttcgtg cagagaataa gcacaatagg
     4861 aaacgggccc gagtgccgct cttagatttt caaccagctt gaattgcctg cgatgagatc
     4921 gttctacgag agcgtgattc ccctgcaatt ctgcgatgag ttgaatgagg cttaccccgt
     4981 catgatttcc gggcatgaaa taggcaggtc gaggcatccg tgcgaggcgt ctggccacat
     5041 ccagtgttcc agcatgtgtt ctgcctggaa ggtccccaac aatcagaacc atatcataat
     5101 ccgagtgttg aatcatgcgc aggtcttctt cgtcgaagct accgtgaatg tcgccaaagg
     5161 ctgcgattcg gatcatgtgc attcatcctc atccgctttt tcgccgtcaa cgctgttgat
     5221 cgtcataatt gcttcctaag aaatgtcatt gtaagatctg cgattctgag tcagagtaaa
     5281 gcaatgaatc tgaagcaaat cgaacaaact ttgactcaaa aaagggccga actggccggt
     5341 cgggcagaca gattgcaaca ggacatcacg cggcagtctt ctcccctgga agaggactgg
     5401 gaggaagcga gcatccagag agagaacgac caggtgctgg acgctctaga cgacgaaact
     5461 cgcgcagagc tccaacagat tgaccgggcc ctgggtcgca tccgagaagg aaaatatggc
     5521 cagtgtgaga aatgttccgg accaattgcg gccgaacgcc tggaggcatt gccctacgcc
     5581 accacctgcg taaagtgcgc gtaaggtttc ctgcttgcga cggcactgga tgacccgtct
     5641 cgttgtctat ggccgtgcaa aaaagaaccg gaggattttt gccgatccgg attttgccgg
     5701 aatcagttgc attttattat aaagattctt ctcaaaatcg cg
//
LOCUS       DAORFF010000211         5725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7607_length_5725_cov_4.654497, whole genome shotgun sequence.
ACCESSION   DAORFF010000211 DAORFF010000000
VERSION     DAORFF010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7607_length_5725_cov_4.654497"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="PKK76_09710"
     CDS             <1..1284
                     /locus_tag="PKK76_09710"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNL68790.1"
                     /translation="FLFGPKNGHPEALKVELEDGGRIKTYSLRYNPEGQNAARPLPGR
                     SGRSIYTGLDVLAESDFALLREKRFALLTNSTGLNRALEPGIDLFLANGIRPEILFEP
                     EHGIFAAEDAAGSEGVRTDSRTGLRIFSLYSSQKKPPSSLLLGLDYLVVDIQNLPVRC
                     YTYITTLSYLLEAAAEAGTPVLILDRPNPFGFIRARGPYPREELRSFVGYAPVPFLYS
                     MTVGEYGHFVAHELLPNLKLSVIRVQGYVRADADAAASNSWINPSPNIPSFESALVYA
                     GVVFFEGTNVSLGRGTTRPFIYVGAPWMHGPELATAMRSLKLPGVQFSNVAFTPSSSL
                     YTGVHCYGVQIHPTSLDFDPIRTGYELMRAVYRLHPGKLTFVWSGNSYGTDRLWGSDS
                     FRMGVINNWDYETFEKTWIRDSESFEEWIAAYRLY"
     gene            complement(1333..1875)
                     /locus_tag="PKK76_09715"
     CDS             complement(1333..1875)
                     /locus_tag="PKK76_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HNL68791.1"
                     /translation="MKQSYLAVLAIAAMLSAPLFADEPHKGACKKDVQTYCKDVQPGE
                     GRIMNCLAENKDKVSAECKKNIGNFVNGFKQACGADEEKFCAKVERGQGRRLKCLNEN
                     SANLSPSCKAKLDEFKASHQAKHEERKAFMDACKADAKAQCKDVKPGQGRIISCLREK
                     ESSLSSACKAELAKVPASVQ"
     gene            complement(1933..4086)
                     /locus_tag="PKK76_09720"
     CDS             complement(1933..4086)
                     /locus_tag="PKK76_09720"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL68792.1"
                     /translation="MRPWLWCFVIGMAVFQPAFAQTASIENSRDLVSLMGDWKFFPGD
                     RTEFAGATFDDSAWLSMRLPQNWHRAGIKYDGIAWFRRKIFVGEGMRDHTIGIVLPLR
                     EGWHEVYVNGRRIDDGASLEMSAPGAHPTRSFPVVIPSSFILFGQENTIAIRSRSYGG
                     VGGFISRTMYAGAMAAVERRYSLSVMWNTVLVTLFFLTGFTHFITFLSRRKEEYYLYF
                     AIFSISIGFIVASIDNLTHFVSDSFLLQHSSVHLPLIFAPSVLVLFAHSLFGRGRRAV
                     TIFSFVWSGLLLLAFFYSVSGPVGYGRYIRYLFPWILCSCALVILYCGTIAVMSIQEG
                     VFTGRIIGLGFLLFAVAVANDILSYLTIIHTPNIVEEGFFCFVVGMSASLSLKYARLQ
                     DSLHNLNRRLHDNIRALQSARSSIEDSEKKYRELVESTNEIIFSLDAGGSITMINQAV
                     GQHLGIPAQELIGQDFQALVYYAGTDSKNSLARHTVRQKLRELLDTRKEVLFRTDFFT
                     RTREPRELIVRLQYVPHNNSYTISGTASVESADELRAVCISERQVYVFGNYVYISDAI
                     SQRVFLGMLKYTDHDSAYAVKVCLREMIINSIEHGNLGITFEEKTEAQNEGTYFSLLL
                     SRQTDPVYGQRNVRLVYSLNEKRALFRITDQGDGFDHQSVLDRDIKSPEFFSLMHGRG
                     IAMARLEFDTIRYNEKGNSVLLIRTFSKHPSMKNV"
     gene            4186..5013
                     /locus_tag="PKK76_09725"
     CDS             4186..5013
                     /locus_tag="PKK76_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68793.1"
                     /translation="MDNVYQSPSLTPPPSGVDGAKFDLDRALNLGWQSFKGQIGLGVA
                     YTIVLALLSVLAVLSCFGILFALPHLAASSAIMGLLMVRGKPELGDMFRPFRAFGAVL
                     GAWLLITIVTQLIAMIFTIPYYAVLLQGVDWSQIQDASGGEAFKEIMQQFQKQQQTFS
                     VAKLGTNLISLLNLPVSLYIGARLMLTYPLIIERGIPPMEAIRQSLAVTRPFQWPLMG
                     FGFITGVIAGLGILGCCVGILFTVPLAFTMHGAAIYQLLGEDTGAPIPGTNTTQTSI"
     gene            complement(5035..>5725)
                     /locus_tag="PKK76_09730"
     CDS             complement(5035..>5725)
                     /locus_tag="PKK76_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNL68794.1"
                     /translation="MTFEVVDLHRSYGSRAILRGVNFILNQGELVCLMGENGAGKTTF
                     IDTVLSRVAPVRGEIRFHDQVLTTTEDRLHFISRTAVLGHDAGVFPDLSAEENLRFFE
                     RAYHGSLTGEGKERISELLSRAGLYSRRHDFVRTFSRGMRQKIGICRAVLHFPEVVFL
                     DEPITALDSAGVDFFFEILSDIRRTAGVFVVTHDPSVFRGEADGFILLAQGVFSPRAK
                     SDLNLKAYQRA"
BASE COUNT         1453 a         1473 c         1382 g         1417 t
ORIGIN      
        1 tttttgtttg gcccgaaaaa cggccatcca gaagccttaa aggtagaact ggaagatggc
       61 ggtcgcatta agacgtattc actgagatac aatccggaag gacaaaacgc tgcacgcccc
      121 cttccaggca gatccggaag atccatctac actggtctgg acgtactggc agaatcggac
      181 tttgcacttc tgcgcgagaa aagattcgcg ctgcttacca attcaaccgg gctcaatcgc
      241 gctctggaac ccggaatcga tctgttcctt gcaaacggaa tcaggcctga aattctgttc
      301 gagccggagc acggcatctt cgcggcagaa gacgcggcag gaagcgaagg agtccgcaca
      361 gattctcgca caggacttcg aatcttcagt ctctatagca gccagaaaaa gccaccgagt
      421 tctctattgc tcgggcttga ttacctggtg gtggacatac agaatcttcc ggttcgatgc
      481 tatacataca ttactacttt gagttacttg cttgaggcag cggcggaggc tggaactccc
      541 gtattgattc tggaccgacc aaatccattt ggattcatac gcgcgcgagg cccgtatccg
      601 cgcgaagagc ttcgtagctt tgttggatat gcaccagttc catttctcta ttctatgact
      661 gtgggagaat acggacattt tgtagcgcac gaacttcttc caaatctgaa gctatccgtg
      721 atccgagtgc agggctacgt gcgcgcggac gcagacgcgg cagccagcaa ttcctggatc
      781 aatccatcgc ctaacatccc gtcattcgaa agcgcgctgg tctatgcggg agtcgtgttt
      841 tttgaaggaa ctaacgtaag cctgggacgc ggaactacgc ggcccttcat ttatgtagga
      901 gctccgtgga tgcatggtcc agaactagcg acggctatgc gatctctgaa attgcccgga
      961 gtgcaatttt caaacgtggc gttcacgccc tcgagttctc tgtataccgg tgtgcattgc
     1021 tacggagtac aaatccatcc aacctctctg gacttcgatc caattcggac cggttacgaa
     1081 ttgatgcgag cagtctacag actgcatccc gggaaattga cgttcgtgtg gtccggcaat
     1141 tcgtatggaa cagatcggtt gtggggaagt gactcgttta gaatgggcgt aatcaacaac
     1201 tgggactatg agacgtttga gaaaacctgg attcgagata gcgaatcgtt tgaggaatgg
     1261 attgccgcct atcgtttgta ttgagagacc agcccgatgg gctggccttc cgtctggttc
     1321 tttctggttc ttctactgaa cagaagcggg aaccttagca agttccgcct tgcaggctga
     1381 actcagactg ctctctttct cgcgaaggca gctgatgatc cttccttgac cgggtttcac
     1441 atctttgcac tgagctttcg catccgcttt gcatgcatcc atgaatgctt tgcgttcttc
     1501 gtgctttgcc tgatgactcg ctttgaattc gtctaacttt gccttacatg aaggactcaa
     1561 gttcgcgctg ttttcattga ggcatttcag acgacgacct tgaccgcgtt ccacttttgc
     1621 gcagaacttt tcctcatccg ctccgcatgc ttgcttaaaa ccatttacaa aattaccaat
     1681 gttcttctta cattcggcag atactttgtc cttattttcc gccaggcaat tcatgattct
     1741 accttcgccc ggttgaacgt ctttgcaata tgtttgaacg tctttcttac atgcaccttt
     1801 gtgcggctcg tctgcaaaaa gaggcgcaga gagcatggct gcaattgcca ggacagctaa
     1861 ataactttgt ttcatggagt ttcctcgtag gagatttcta caggcattag atcggacccg
     1921 ctacggcagg gttcaaacat ttttcatgga aggatgcttt gaaaatgtgc gaatcaggag
     1981 aacgctgttt cccttttcgt tgtagcgaat cgtgtcaaat tccaaacgcg ccatggcgat
     2041 tccacgaccg tgcatgagac taaagaactc ggggctcttg atatcacgat caagaacgct
     2101 ctggtggtca aaaccgtccc cctgatccgt gattctaaaa agtgcgcgct tttcgttcag
     2161 ggaatagacc agcctgacgt tacgttgacc atacacaggg tctgtttgcc tggacaaaag
     2221 aagactgaag taagtccctt cattttgcgc ttctgtcttt tcctcgaagg taatccccag
     2281 atttccgtgt tcaatggagt tgatgatcat ttcacggaga caaaccttta cagcgtatgc
     2341 tgaatcatga tccgtgtact tcagcatgcc cagaaagacc ctctgactga tggcatcaga
     2401 aatatacaca taattcccga acacgtatac ctgcctttct gagatacaca cggcgcgcag
     2461 ctcgtctgcc gactccacgg aggctgttcc ggatatcgta taactattgt tatgtggaac
     2521 atattgcagg cgaacaatca gctccctcgg ttctctagta cgcgtaaaga aatcagtgcg
     2581 aaataaaact tccttgcgcg tatccagcaa ctcgcgtagc ttctgacgca ctgtgtgcct
     2641 tgcgagggaa ttcttactgt cagttccagc atagtacacc agagcctgga aatcctggcc
     2701 gatcagttcc tgggcgggga tgcccaggtg ctggccgacc gcttgattga tcatggtaat
     2761 gctgccgcct gcatccaggg aaaaaatgat ttcgtttgtg ctttcgacaa gttcgcgata
     2821 cttcttttct gaatcttcga tactggatct cgcggattga agcgcgcgaa tattgtcgtg
     2881 caggcgcctg ttcaagttat gaaggctatc ctgtaacctg gcatatttga gagacagaga
     2941 agcggacatc cccactacga agcaaaaaaa tccctcttcc actatgttgg gagtatggat
     3001 gatggtgaga taagacagaa tgtcattggc cacagctact gcaaatagta gaaatcccag
     3061 tccaatgatg cgaccggtaa aaacgccttc ctgaatactc atgactgcga tggttccgca
     3121 gtacagaatc accagagcgc aactgcatag aatccatgga aacaggtacc gaatgtaacg
     3181 tccgtatcca actggaccag aaaccgaata aaagaatgcg agaagcaaga gtccggacca
     3241 tacgaaagag aaaatggtaa cggctcgacg ccctcggcca aatagactgt gcgcaaacaa
     3301 tacaagtaca gaaggcgcaa agatcaaagg caggtgaacg cttgagtgct gaagcaggaa
     3361 ggaatccgac acaaaatgag ttagattgtc aatcgaagct acaatgaacc cgatcgaaat
     3421 ggagaatatt gcgaaataga ggtagtattc ctcctttctc ctgcttaaga atgttatgaa
     3481 atgagtaaat cctgtgagga agaagagtgt cacaagaacc gtattccaca tgacagaaag
     3541 cgaataacgt ctctccactg cagccatagc acccgcgtac atagtacgcg aaataaaacc
     3601 acctacgcca ccatagcttc gcgatcgaat tgcgattgtg ttttcctgtc cgaacaaaat
     3661 aaaagaggat ggaattacta cgggaaacga tctagtcggg tgcgcacccg gcgcggacat
     3721 ttcgagagag gcaccatcgt ctatgcgacg tccgttcaca tatacttcat gccagccttc
     3781 gcgcaagggt aggacaattc cgatggtatg gtcacgcatc ccttcaccga cgaaaatctt
     3841 tctgcgaaac catgcaatcc cgtcgtattt gatgcctgca cgatgccagt tctgtggcaa
     3901 cctcatgctc agccaggcag agtcatcaaa ggtggctccc gcgaactctg tgcgatcacc
     3961 cggaaagaac ttccaatcac ccatgaggct gaccaggtca cggctgtttt caatcgatgc
     4021 ggtttgtgca aaggccggct gaaacacggc catgccgatc acaaaacacc agagccacgg
     4081 ccgcatgatg tcctcctgtt gcactgaaat cgggttgggt caagccaaat agaccacttg
     4141 aacaggattt gcttgcaact cctggcccgg actccccgct tgctcatgga taacgtttac
     4201 caatcaccgt cgctgactcc tccgccttcc ggagtagacg gagcaaaatt cgatctcgat
     4261 cgcgcgctca atctgggctg gcagtccttt aaaggccaga tcggtcttgg agtagcatat
     4321 acaatcgtgc tggcactgct gagtgttctc gcagtgcttt catgttttgg cattctgttt
     4381 gcattgcctc acctggccgc ttcttctgca atcatgggac tgctgatggt tcgcgggaaa
     4441 ccagaactgg gagatatgtt tcgcccattt cgcgcattcg gtgcagtact cggcgcatgg
     4501 cttctgatca cgattgtaac gcaactgatc gcgatgattt tcacgattcc atactacgcg
     4561 gttttgctcc agggcgtaga ttggagccag attcaagatg cttcgggagg cgaagcattc
     4621 aaagaaatca tgcaacaatt tcaaaagcaa cagcagacct tttcagtagc aaaacttggg
     4681 accaatctaa tctctcttct gaacctgcct gtttcattgt acattggtgc aaggctcatg
     4741 ctgacctatc ctttgatcat tgaacgcgga attccaccca tggaagcaat caggcaaagc
     4801 cttgccgtta cgcggccatt tcagtggccg ctgatgggtt tcggattcat tactggtgtg
     4861 atcgcagggc ttggtattct gggttgctgc gttggtattt tgttcactgt gcccctcgca
     4921 ttcaccatgc acggagccgc catctatcag ctgcttggcg aagatacagg cgctcccatc
     4981 ccgggcacta acacgacgca gacttccatc taggagatca ttcgccgcat aacgtcacgc
     5041 acgctggtag gctttcaaat tcagatcgct cttcgcgcgc ggggaaaata ctccctgcgc
     5101 gagaagaatg aatccatccg cctcaccgcg aaatacagaa gggtcgtggg tcacaacaaa
     5161 gacccccgca gtccgtctga tatcagacag tatttcaaaa aagaaatcca ctcccgctga
     5221 atcaagggca gtgattggct cgtccagaaa tacaacctcg ggaaaatgaa ggaccgcgcg
     5281 gcatattccg atcttctgcc tcattccgcg agaaaacgta cgaacaaaat catgccgtct
     5341 ggaatagaga ccagcacgcg acaaaagctc gctgattcgt tctttgcctt cccccgtcaa
     5401 tgaaccatga tacgctcgtt caaaaaaacg cagattctcc tctgcagaaa gatctgggaa
     5461 cacaccggca tcgtgaccta gaactgccgt gcgagaaatg aaatgcagtc tatcttctgt
     5521 agtggtaaga acctgatcat ggaatcgaat ttccccgcga accggagcca ctctgcttag
     5581 aaccgtgtcg atgaacgtgg tcttgccggc tccattttct cccatcaggc aaacgagctc
     5641 gccttgattg agtataaaat ttacgccgcg aaggatggcg cgtgatccat aactgcgatg
     5701 cagatcaaca acttcaaacg tcatt
//
LOCUS       DAORFF010000212         5725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7609_length_5725_cov_3.467549, whole genome shotgun sequence.
ACCESSION   DAORFF010000212 DAORFF010000000
VERSION     DAORFF010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7609_length_5725_cov_3.467549"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PKK76_09735"
     CDS             complement(<1..386)
                     /locus_tag="PKK76_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNL68795.1"
                     /translation="MLGFKKKPKHDHSKPIVIIMAGGKGERFWPRSTKAIPKQLQKVY
                     SNRTLLDETWARALSITSSQNIYIGCNAHLRDAIIKTHPEIKKHNFIIEPEGKNTAPI
                     LALAAVQLNQEHPEAVQIILSADHFI"
     gene            complement(414..1358)
                     /locus_tag="PKK76_09740"
     CDS             complement(414..1358)
                     /locus_tag="PKK76_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68796.1"
                     /translation="MISRFFRVFFWAILLSSIFSWIYLGIVSATARQAIIVLHRKRGM
                     PDLVAPGESQFFPGRIFPGRVEIHPVDIGPRMLVVDYKKGLYQTEMLGLDDSFYIRFK
                     FRFRYTLQPEKLPDLFLKMEGLDWKALEPYLQLRLSEMLTKRLLVIFPADNDLGQVPR
                     RLNDYFNGPALEEMKSILGPEGIEIQNLVVENIYVPDAGRYTAILGGGQGILNQKLER
                     IRKRDEALARRDAESLTDEATYARLETIGKLLIRYPGLKDYLAVDRLGSDVKVMVMPY
                     ERWFNSGRPTADSLLDKEEPAATQNGKRGPRFENLTPP"
     gene            complement(1355..1600)
                     /gene="hfq"
                     /locus_tag="PKK76_09745"
     CDS             complement(1355..1600)
                     /gene="hfq"
                     /locus_tag="PKK76_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNL68797.1"
                     /translation="MANKNNIQDLVLNSARKEKMEITVYLTNGVPLKGRVVSFDNFTV
                     VIEQDSKQSLVYKHAISTIVLSKPLNLDFSEDGAVKG"
     gene            complement(1630..2505)
                     /gene="miaA"
                     /locus_tag="PKK76_09750"
     CDS             complement(1630..2505)
                     /gene="miaA"
                     /locus_tag="PKK76_09750"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNL68798.1"
                     /translation="MPVLFGPTGSGKTAALCRLTDVYGDKFEVIACDSRQLYRGLEIT
                     SAAPTSDELAIIPHHLISILDPNEETTAFRFRRLAAEAVTDILSRGHVPVLSVGTGFY
                     FKAVRTRTQVSTPDASVREAIQSMTPSQRLSELSLKRPDLLQKGGGRIHENDDYRIGR
                     ALENLVPGREDLLPEFPCEFLSFYLDISVSDLNERLRLRVLKMIELGLVEELRGVFDR
                     FGKCPGLSLIGFDLVQLLLTGELSANDFAERLWIEHRQYAKRQRTWFRREKIEARGSW
                     ADFVSWVGLFVDPRR"
     gene            complement(2514..3119)
                     /gene="gmk"
                     /locus_tag="PKK76_09755"
     CDS             complement(2514..3119)
                     /gene="gmk"
                     /locus_tag="PKK76_09755"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNL68799.1"
                     /translation="MTAPGLLQVSSMDRAGHAVVISSVSGGGKTSLIHLLTRMYPHLE
                     TVITATSRPIREGEINGVHYFFYSRDEFEARIARGEFLEHATVHGNHYGVPLDQVRGK
                     MDQGKSVILNIDVQGARRVKEILGDRVITFFLLPPNRPEWERRLRGRGTDSEQVIQHR
                     LAEGLRELEEARGYDYRIINDVLERAAGEISVILKEREVLH"
     gene            complement(3123..3941)
                     /locus_tag="PKK76_09760"
     CDS             complement(3123..3941)
                     /locus_tag="PKK76_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNL68800.1"
                     /translation="MADIPDLTSEQFRKFADLIRREASITLREQKIVLLSNRLRRRAL
                     ATGCANFDEYFAFVTSKPEERRPFLEAITTNESYFWRSTASFDLMKKVALPQLLKAYP
                     KAPIQIWSAGCSTGEEPYNIAMEVTEAMKSLGVFPYQVIATDISARVVDFAKVGRYSG
                     RKIEKIPPLLLYRYFRADPESKGQFLVRDDLRTKIDFRVENLFESTPRNVHCIFCRNV
                     MIYFQKADQEILIRRFYDALVPGGFLLVGHSESLHVLQADFTPVHCPDGVIYRK"
     gene            complement(3943..4755)
                     /locus_tag="PKK76_09765"
     CDS             complement(3943..4755)
                     /locus_tag="PKK76_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68801.1"
                     /translation="MEFLVFALLNLVTGLVLYVLFSLRMQAWQNKIRAAHPLQKDVEE
                     NIRQGLQAMNAYLEEMQEIRANFYQLVRHAEDLKKEETRKPRKKKREEPELKSLLPEE
                     EEQEDHVSRILDKIGPDTVDLTRPAAPQPRSQPPVQQPEPSAGILQGIGRFAASIFGV
                     KTDPFAASIGKATPAEEALSIDVPQPTIRPPVSLNVEPARESVGLPSASDATPASAPI
                     TQDPNAWIRSLLAGGMKAADVSRVTGRSLAEIDLIGSLPSMPPEPRRKKLGD"
     gene            complement(4758..5087)
                     /locus_tag="PKK76_09770"
     CDS             complement(4758..5087)
                     /locus_tag="PKK76_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68802.1"
                     /translation="MRLLDLQVSYQALPEQARLQGMEHAAGAYRQVAAMALARQENLE
                     RPEKVIQAASTTSASMAAIDPREKRMQSQQQPSKSSREVETFIYNERGIKRKPPTPVG
                     SKLDLFI"
     gene            complement(5084..>5725)
                     /locus_tag="PKK76_09775"
     CDS             complement(5084..>5725)
                     /locus_tag="PKK76_09775"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNL68803.1"
                     /translation="QSARVNVAEDVVVQEGILHSEVDAGGKVVCNGRRAQIVGGAIRA
                     KKEVRARMIGSPSYTGTEITVGTDPRVLKQFDELRALSKDTEDKLSRAKKSLATLNAR
                     KKADVDSFSPAQEEQLQKAEDASQKLTARTKEIAEEMGKLEEYMNQLSAQGKVHIEKE
                     VFPNVTVTIRNVSQTFSDTYRAVTLTFENGVVKFNKLEKAEEKAAPARGGRRK"
BASE COUNT         1350 a         1475 c         1425 g         1475 t
ORIGIN      
        1 tgaatgaaat gatccgcact caaaatgatc tgaactgctt ctggatgttc ctggttgagc
       61 tgtactgcgg ccagagcgag aatgggtgcc gtgttctttc cttccggttc aatgatgaaa
      121 ttgtgcttct tgatctccgg atgtgtcttg atgatcgcat cacgcaggtg agcattgcat
      181 ccaatgtaaa tgttttgcga cgaagtgatg gataaagcgc gcgcccaggt ttcatctagc
      241 aatgtccgat tggaatacac tttttgaagc tgctttggaa tggcttttgt ggacctgggc
      301 cagaaacgtt cgccctttcc tcctgccatg atgattacaa ttggttttga gtgatcgtgt
      361 tttggtttct ttttaaatcc aagcatagca aacttccggg agattttcag agatcaaggt
      421 ggagttagat tttcgaatct gggcccgcgt ttcccattct gagtggccgc aggttcttct
      481 ttgtcaagca ggctgtctgc ggttgggcgc ccggaattga accatctttc gtatggcatg
      541 accatcactt tcacatcgct accgaggcga tccactgcca ggtaatcttt aagacccggg
      601 taacgaatca gcaatttgcc gattgtttcc agtcgggcat atgttgcttc atccgtcaaa
      661 gattcggcgt cgcgacgcgc gagagcttca tctcttttgc gaatgcgttc cagtttctga
      721 ttcaagattc cctgaccgcc accaagaatc gccgtataac gtccagcatc aggaacataa
      781 atattctcaa ctaccagatt ctgaatttca atcccttccg gtccaaggat tgatttcatt
      841 tcttcgagag ccgggccatt gaagtaatcg ttcagacgac gcggcacctg gcccagatcg
      901 ttatctgcag gaaagatcac aagaagtctc tttgtgagca tctctgagag gcgaagttga
      961 agatacggtt ccagggcttt ccagtccaga ccttccattt tcaaaaacaa atctggaagt
     1021 ttctcaggct ggagagtata acggaaacgg aacttaaatc ttatgtaaaa cgaatcatcg
     1081 agaccgagca tctcggtctg gtaaaggcct ttcttgtagt ccacaacgag catgcgaggg
     1141 ccaatgtcca ctggatgaat ctcaacgcgg cctggaaaga ttctacctgg aaagaactga
     1201 ctttcacctg gagcaaccag atccggcatt ccgcgcttgc ggtgcagtac aatgatggcc
     1261 tgacgcgcgg tcgcagaaac aatgccaaga tagatccagc tgaagatcga agagagcaga
     1321 atggcccaga aaaaaacgcg gaagaaacgc gaaatcatcc tttaactgcg ccatcctccg
     1381 agaagtccag gttcaacggc ttggagagaa caatggtaga gattgcatgt ttgtatacga
     1441 ggctctgctt ggaatcctgt tcgatgacta cagtaaagtt gtcaaaacta acgactctgc
     1501 ctttcagtgg aacgccattg gtcaggtaga cggtgatctc catcttttcc ttgcgcgcgg
     1561 aattcagcac aagatcctgt atattgtttt tgttggccac ggcggttctc cgaaatacag
     1621 gttgcgatct taacgccgcg ggtcaacgaa taatccgacc caggacacaa aatcggccca
     1681 ggagccacgt gcttctatct tctctcgcct gaaccacgta cgttgcctct tcgcatattg
     1741 cctgtgctcg atccacagac gctctgcaaa atcattggca ctcaattctc ctgtaagcag
     1801 caactgcacc agatcaaatc ctatcaggga aagccccggg cattttccaa agcgatcaaa
     1861 cacaccgcgc aattcttcca ccagacccag ttcgatcatc ttcaaaacgc gcagacgaag
     1921 tcgttcgttc aaatcggaca cagaaatatc cagatagaag gaaagaaatt cgcaagggaa
     1981 ttctggaaga aggtcctcac gtcctgggac cagattctca agcgccctgc ctatcctgta
     2041 atcgtcattc tcatgaatgc gtccgccgcc cttttgaaga aggtccgggc gtttcaaaga
     2101 aagttccgac aatcgctggg acggtgtcat tgattgaatg gcctctcgca cggaagcgtc
     2161 tggagtggag acctgtgtgc gagtgcgcac ggctttgaaa tagaaaccag tcccgacaga
     2221 caacactgga acatggcctc tagagagaat atctgtaacc gcctctgccg caagcctgcg
     2281 aaagcgaaac gctgtagtct cttcgttcgg atccagaatt gaaatcaaat ggtgcggaat
     2341 gatagccagt tcatccgacg ttggtgcagc agaagtgatt tcaagaccgc ggtagagttg
     2401 cctcgaatcg catgcgatga cttcgaattt atctccgtac acatccgtca ggcgacatag
     2461 agcggctgtc ttgccagaac ctgtaggacc aaagagtaca ggaatgaccg gtttcagtgc
     2521 agaacttctc tctctttcaa gattacactg atttctccgg cggcgcgctc aagtacatcg
     2581 ttgatgattc tgtagtcata tccgcgcgct tcttcaagtt cgcgcaagcc ttccgcgagg
     2641 cgatgctgga tgacctgttc gctgtccgtt ccgcgtccac gaagcctgcg ttcccattcc
     2701 ggacgattgg gtggaagaag gaaaaatgta atcactctgt ctcccagaat ctccttaact
     2761 cttcgcgctc cctgcacatc aatgtttaga atgacgcttt ttccctggtc cattttgccg
     2821 cgcacctggt cgagtgggac tccgtagtga tttccgtgca cagtcgcgtg ttccaggaac
     2881 tctccgcgag caattcttgc ttcaaactca tcacgcgagt agaagaagta gtgcactccg
     2941 ttaatttccc cttctcgaat gggtctactg gtagcggtga tgactgtttc caggtgcggg
     3001 tacattcgcg tgagcaaatg aatgagggag gtctttcctc ctcccgatac ggaagagatg
     3061 accaccgcat gccccgcccg gtccatagat gatacttgaa gaaggcctgg cgctgtcaag
     3121 tttcacttac ggtagatcac gccatccgga caatggaccg gagtgaaatc agcctgaaga
     3181 acatggagcg attcggagtg gcctacgagc aaaaagccgc caggtacgag agcatcatag
     3241 aagcggcgaa tcagaatctc ctggtccgcc ttctgaaaat agatcattac gtttctacaa
     3301 aagatacaat gaacgttcct gggtgtggat tcaaaaaggt tttctacgcg aaagtcgatc
     3361 ttcgtgcgaa gatcgtctct taccagaaac tgacccttcg attccgggtc cgcgcgaaaa
     3421 tagcgataca aaagcaatgg cggaattttt tcgattttgc gtcccgaata gcggcccact
     3481 ttcgcaaaat caaccactct ggccgagatg tctgtggcaa tcacctggta gggaaacacg
     3541 ccgagcgatt tcatcgcttc tgttacttcc atggcaatgt tgtaaggctc ttctcccgtg
     3601 gaacatccgg cggaccagat ctgaatggga gcttttgggt atgctttgag gagctgcgga
     3661 agcgcgactt ttttcatgag atcaaagctg gccgtgctcc gccagaagta ggactcattt
     3721 gtagtgatcg cttcgaggaa tggccttcgc tcctctggct tagatgtaac aaaagcaaag
     3781 tactcatcga aattcgcgca gcctgttgcc agggcgcgcc tgcgcagacg gttcgatagt
     3841 aagacgattt tctgttcccg caaagtgatc gaggcttcac gtctgatcag gtctgcgaat
     3901 ttgcgaaatt gctcagacgt gagatccgga atgtcagcca cgtcagtcgc ctaatttttt
     3961 tctgcgaggt tcagggggca tggaaggcaa agacccgatc aggtcgattt ctgccaggct
     4021 ccgacccgtt acacggctta cgtctgcagc tttcattcca ccggcaagaa gacttctgat
     4081 ccacgcattc gggtcctgag tgatcggagc ggaggcgggg gtagcatccg acgcggaagg
     4141 aaggccaacg ctttctcgcg caggttccac gttaagcgat acaggagggc gaattgtagg
     4201 ttggggtaca tcgatagaca gcgcttcctc tgcaggagtt gccttcccga tggatgcagc
     4261 aaatggatcc gtttttacac cgaagatgga ggccgcaaat cttccaatgc cctgaagaat
     4321 tccagcagat ggttctggct gctgaacggg aggctgacta cgaggttgcg gagcggcagg
     4381 acgcgtcaaa tcgacagtat ctggtccaat cttatctaga attctgctta catgatcttc
     4441 ttgctcttct tcttccggaa gaagtgactt aagttccggc tcttcgcgct ttttctttct
     4501 tggcttgcga gtctcttctt ttttgagatc ttccgcatgg cgaacgagct gatagaaatt
     4561 cgcacgaatc tcctgcatct cttcgagata agcgttcatg gcctgaagac cctgacgaat
     4621 attttcttct acgtctttct gcaaaggatg ggcggcgcga atcttatttt gccacgcttg
     4681 catgcgaagg gaaaagagca cataaagaac aaggcccgta accagattga gcaatgcgaa
     4741 gaccaggaat tccattctta aataaacaaa tcgagttttg aaccgactgg agtcggaggt
     4801 ttacgtttga ttccgcgttc gttataaatg aacgtttcta cttcccgcga agattttgat
     4861 ggctgctgtt gcgattgcat gcgcttttcg cgcggatcaa tggcagccat agaagcactc
     4921 gtcgtcgacg ctgcctggat taccttttca ggacgctcca gattctcctg acgcgcaagc
     4981 gccatggcag ccacctggcg atacgctccg gccgcatgct ccatcccctg caggcgggcc
     5041 tgttccggaa gtgcctgata cgatacctgt aagtccagga gcctcatttt cttctgccgc
     5101 cgcgcgcagg cgccgctttc tcttcagctt tttcaagttt gttgaatttt acaaccccgt
     5161 tttcgaacgt cagagtcacg gcccgatatg tatccgaaaa ggtctgggaa acattgcgaa
     5221 tggtgaccgt aacattgggg aagacttcct tctctatatg aacttttccc tgtgcagaca
     5281 gctggttcat gtattcttcg agcttgccca tttcttctgc gatttctttg gttctggcag
     5341 tcagcttctg gcttgcgtct tccgccttct gcagttgctc ttcctgcgcc ggtgaaaagc
     5401 tgtccacgtc tgctttcttg cgagcgttca aagtggccag agacttcttc gcacggctca
     5461 gcttgtcttc tgtgtctttg gaaagcgcgc gcagctcatc aaactgcttg agcacgcgag
     5521 gatctgtgcc aactgtgatt tctgtgcctg tgtaagacgg ggagccaatc atccttgcgc
     5581 gaacttcttt cttcgcacgg attgctccgc ctacaatttg cgcacgacgg ccattgcaga
     5641 ctacctttcc gcccgcatct acctcactgt gcaaaatccc ttcctgaacc acaacgtctt
     5701 cagcaacatt gacgcgcgca ctctg
//
LOCUS       DAORFF010000213         5706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7650_length_5706_cov_3.308264, whole genome shotgun sequence.
ACCESSION   DAORFF010000213 DAORFF010000000
VERSION     DAORFF010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7650_length_5706_cov_3.308264"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKK76_09780"
     CDS             <1..356
                     /locus_tag="PKK76_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68804.1"
                     /translation="IPVGTIVKEGPRQGQARTFFVKPFAQLMQLDSVTVVIKKPAAWA
                     RKNDEDKLEEFLKTEFGAPEYPETLMRLDSGRDRGPKQTQDPGEQTTGTDQQPATQGQ
                     QNEPRRIQNVNPQTR"
     gene            358..876
                     /gene="mreD"
                     /locus_tag="PKK76_09785"
     CDS             358..876
                     /gene="mreD"
                     /locus_tag="PKK76_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNL68805.1"
                     /translation="MFLNFAVVAIGILVSFFLKGINFLSLDLESVGGGILYPDFLLIL
                     VIFFALRRGDFSALWVGFFSGLLEDSGLLSFADKTGEFVPIIGTHALVYCLIGFILGK
                     VHKLMDKDRVPTIILIVFVTSVVERIIVWLLVGVLEDFNKSFSVLGPSVYTALFAPIW
                     IAGLSWVYKMQE"
     gene            877..2784
                     /gene="mrdA"
                     /locus_tag="PKK76_09790"
     CDS             877..2784
                     /gene="mrdA"
                     /locus_tag="PKK76_09790"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNL68806.1"
                     /translation="MSRSASEFRLEQQFQTRVYIFTGFMILVGTVLTLQMGNLQLLHG
                     AENRRMAKKFVSRQEFTVAPRGLILDRTTDPANPLVKNLITTDFVVFPSRFENLEEGK
                     GYIREFCRIMGRNYANYEQYLTPEAWKVLVRRNESITLLTRMDTAEHERLGELRMVTE
                     YGSFVTNHLRYYTMGPAAAHVTGYIGLPSRSELDRGLALPYQMLGKGGIEARYDSDLR
                     GTDGVRIRHRFVDEEEQLAESEQGNSLVLTIDRRAQAAAYNSLKNSGRRGAVVAMRPS
                     TGEILALVSYPAFDPNILSSGSADQRSRHTKTVQDYEGFLNLAIQAKFPPGSTFKPLV
                     AIAALENSAENHVTEDTSFHCAGSWLLPSSVAGVPPARFNCWNAGHGTNNLIGAIAQS
                     CTVYFYQLGYKIGPNAMIHFAKATGLDKKTGVDLPGEIQGFVPDQRWKQITWSSRWYD
                     GDTVNLAIGQGFLEVTPIELAVLYSAMANRGKIYRPYLIKEVRDPVSNRVLRQVRPDL
                     IHEVPISQKSLDIVQEGMRAVVSRGTAQHLNRPDYPPIAGKTGTVQTRSRIKAANHAW
                     FASFAPYGAPIEEQIVVVVFVEFGMGGSAAAAPIAGDVFHAVFPDWTPTVHPQIKPDR
                     KEETPPNGKPQ"
     gene            2781..4307
                     /locus_tag="PKK76_09795"
     CDS             2781..4307
                     /locus_tag="PKK76_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNL68807.1"
                     /translation="MINRYKLDVYLLGAAVVVVLAGIFTLYTQEYQLAEGSGRWYKQL
                     IFFGLGLIICLGIRRMNYQVLEAYAVPAYLGSLFLLMLVLVFGATIKGAKSWFRLGPI
                     NVEPSEIARLATILLLAKYLQLKERDMGKIPTLLIAFGICLLPMLLIVVQPAFGDAFA
                     FTPILLSMLFIAGADMYHIGSVVVFFSVAISVPLYIEYHNITLVDPLVGHLSDLGKTD
                     LLPAVRLLKTDIWKFAAKGKIPVGIEGADKDYLVGLAGNQNLRASLVEAVNSVRSDSG
                     GLLLRLLENDTLLLALGIFFALSALVLLILRLTQGTSLANLRKLYIPFGVIGMSLLSA
                     VAVHTTFSFKYHQIARVTAFINPDRFPRDLAYQTRASRAAIGSGELSGRGIFQGDMTT
                     GERPLVPEAYTDFIFTAWCERTGFLGGTLILLLLLSIPLRGMLVSFESRDRFASLLSA
                     GISFMFFFHILMNVGIAVGLLPVTGLPLSFMSYGGSHLITAMVSLGILLSVARRRFAN
                     "
     gene            4375..5061
                     /locus_tag="PKK76_09800"
     CDS             4375..5061
                     /locus_tag="PKK76_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68808.1"
                     /translation="MSSERYEQLHDLLAGYESSILKLESDLQECQGALPGMETTAAEM
                     TPLLPDRAGFARERSIRLMHRTARLHKLFGEFRKEFQILHRHMDKIRRDARLTLIPLP
                     EERKEVRKADYTLFRLGDMSFCVMDRPVRVEQDVSVSNKIADATLDLFPGRDTISIFA
                     PGKSRLGLLYFGEGKTEQAIWFEEMLEPVIESSHMVQLQPAGVAHPRVRGKFRFRGVD
                     YYVVTLSDTE"
     gene            complement(5065..5496)
                     /locus_tag="PKK76_09805"
     CDS             complement(5065..5496)
                     /locus_tag="PKK76_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNL68809.1"
                     /translation="MASNDLILAMGSGFRGAMDIVRENMQKKDKQSTLALSILIHVER
                     QPGISQGELGRMLRRDPMTMSQAVRALQNSSLITSQADGEDRRIKRLNVTKKGKTFAQ
                     SMNGGESKLINALGKEWGKGRVNQFAKDLAEFNEFLTTYNS"
BASE COUNT         1295 a         1352 c         1490 g         1569 t
ORIGIN      
        1 gaattccggt gggcacgatt gtgaaagaag ggccgcgcca gggccaggct agaacgttct
       61 tcgtgaagcc cttcgcacaa ctcatgcagc ttgattcagt cacagttgta atcaagaagc
      121 cagcggcgtg ggcccgtaag aacgatgaag acaaactcga agaatttttg aagacagagt
      181 ttggtgcgcc agagtatcca gagacgctca tgcgtcttga ttccggtcgt gaccgcggac
      241 caaagcagac acaggacccc ggagaacaga ccactggcac agaccagcag cccgccactc
      301 aggggcagca gaatgaaccc agaaggattc agaacgtgaa tccgcagacg cgctgatatg
      361 tttctcaatt tcgcagtagt tgcaattggt atacttgtat ctttcttctt gaaagggatc
      421 aatttcctga gcctggatct ggagtccgtt ggcggtggaa ttctctatcc tgatttcctt
      481 ctgattctcg tgatcttctt tgcccttcgt cgcggggatt tttctgctct gtgggttggc
      541 tttttttcgg ggcttctgga agattcaggt ctgttgagtt tcgcggataa gaccggggaa
      601 tttgttccga ttattggaac gcatgcgctc gtgtattgcc tgatcgggtt tatcctgggc
      661 aaagttcaca agcttatgga taaagatcgc gtgccgacca tcatcttgat tgtctttgta
      721 acaagtgtgg tcgaacgaat cattgtctgg cttctggttg gagtgttgga agattttaac
      781 aaatcgtttt cagtgctcgg tccgagtgtt tatactgcat tgttcgctcc aatctggatt
      841 gcaggcctga gctgggtcta caagatgcag gaatagatga gtcgttccgc ctcagagttt
      901 cgtttagagc agcaatttca gacgcgcgtg tatattttca cgggcttcat gattctggtt
      961 ggaacggttc tgactctgca gatgggaaat ttgcagcttc tccacggcgc ggaaaacaga
     1021 cgcatggcaa agaaatttgt cagccgtcaa gaattcacag tcgctcctcg cgggttgatt
     1081 ctagatcgca cgacagatcc tgccaatcca cttgtgaaga acttgatcac cactgatttc
     1141 gtggtgttcc cttctagatt tgaaaatctg gaagaaggca aaggctacat acgcgaattc
     1201 tgcagaatca tgggtcgcaa ttatgcgaac tatgagcaat accttacgcc ggaagcgtgg
     1261 aaagtactcg taagaagaaa tgaatccatt actctcctga ctcgcatgga cacagcggag
     1321 catgaaaggt taggcgagct tcgcatggta acagagtacg gatctttcgt taccaatcat
     1381 ttgcgttatt atacaatggg cccggctgcc gcacacgtaa ctggatacat tggtcttcca
     1441 agcagatcgg aactggatag gggtctggca cttccctatc agatgctcgg caaaggtggg
     1501 attgaagcgc gttacgactc tgatcttcgc ggaacggacg gcgttcgcat tcgccatcgc
     1561 ttcgtggatg aagaggagca gctggcagaa tccgaacagg gaaacagcct ggttctgacg
     1621 atcgatcgtc gtgcgcaggc cgcggcttac aattcactca agaactcagg ccgtcgcggc
     1681 gccgttgttg ccatgcgccc atctacaggt gaaattctgg ccctggtatc ctatcctgct
     1741 tttgatccta acattctatc cagcggttct gcagaccagc gctcgcggca tacgaagacg
     1801 gttcaggatt atgaaggttt cttaaatctt gcgattcagg caaagtttcc gcccgggtct
     1861 acgttcaaac cgttagtcgc tattgctgca cttgaaaact ccgctgagaa tcatgttacg
     1921 gaagatactt cttttcattg tgcgggaagc tggcttcttc cttcttctgt agctggagtg
     1981 ccgcctgcta gattcaactg ctggaatgcg ggtcacggaa cgaacaatct gatcggcgcg
     2041 attgcgcaat catgcacagt gtacttctat cagctgggtt ataagattgg cccgaacgcg
     2101 atgattcatt ttgcaaaggc caccggtctg gataagaaga ctggggtgga cttgcctgga
     2161 gagattcagg gatttgttcc agaccagaga tggaaacaga tcacctggtc cagtcgctgg
     2221 tatgacggag acacagtgaa cctggcaatc gggcagggct ttctggaagt tacgccaatc
     2281 gaactggcgg ttctgtattc tgcgatggcc aatcgcggaa agatttacag accatatttg
     2341 atcaaagaag tgcgcgaccc tgtgagcaat cgtgtcctca ggcaggttcg ccctgatttg
     2401 attcacgaag ttcctatttc gcagaagagc ctggatattg ttcaggaagg aatgcgagcg
     2461 gtggtttcgc gcggaaccgc ccagcatttg aaccggcctg attatcctcc gatcgccgga
     2521 aagactggaa ccgtgcagac gcgaagcaga atcaaagcgg ccaaccatgc gtggtttgca
     2581 tctttcgcac cctacggcgc accgatcgaa gagcagattg tagtggttgt gtttgttgaa
     2641 tttggtatgg gtggatctgc ggctgcagcc cctatcgccg gtgacgtatt tcacgccgta
     2701 tttcctgatt ggacgcctac agttcatcct caaataaaac ctgatcgaaa agaagaaacg
     2761 ccgcctaacg gtaaaccgca atgatcaatc gctacaaact ggatgtgtat ttactgggcg
     2821 cggctgttgt agttgttctg gccggtattt tcactctcta tacgcaggaa taccagctgg
     2881 cagagggttc tggacgctgg tacaaacagc tgatcttctt tgggctcggt ttgatcattt
     2941 gccttggaat tcgcagaatg aactatcagg tactcgaagc gtatgcggtg cctgcgtatc
     3001 ttggatctct gtttttgctc atgctggtgc ttgtattcgg ggccacaatc aagggagcaa
     3061 aatcctggtt tcgtcttggg ccaatcaacg tggaaccgtc tgaaattgcg cgcctcgcca
     3121 ccatcttgct tttagcaaag tatctgcaat tgaaagagag agacatggga aaaattccca
     3181 ctctactcat tgcctttggt atttgtcttt tgccaatgct tctgatcgtg gtccagcctg
     3241 cgttcggtga tgctttcgca tttacgccga ttctactcag catgcttttt attgcgggtg
     3301 cggatatgta ccacattggc tctgtggtgg tgtttttctc ggtggccatt tctgttcctc
     3361 tctacattga atatcataac attactcttg tggatcctct cgtgggacat ctgtctgacc
     3421 ttggaaagac agatctattg cccgcagtgc gtcttcttaa aacggatatc tggaaatttg
     3481 cagcaaaagg aaagatccct gttggcattg aaggggcaga caaggactac ctggttggtc
     3541 ttgccggaaa tcagaatctt cgcgcatccc tggtagaggc tgtgaattct gttcgatctg
     3601 attcaggcgg attgcttctt cgccttctag agaacgatac acttcttctg gctcttggaa
     3661 ttttcttcgc actcagtgcg ctggttctac tcattcttcg tctcactcaa ggaacttctc
     3721 tcgccaattt gcgcaaactc tacattcctt ttggtgtaat tggaatgtcc ctcttgtctg
     3781 ctgttgcggt tcacaccaca ttctctttca agtatcatca gattgctcga gtgaccgcgt
     3841 tcatcaaccc ggatcgattt ccgcgagacc tggcgtatca gactcgcgca tctcgcgcgg
     3901 ccattggatc cggcgaattg tctggtcgag gaatttttca gggagatatg actacgggag
     3961 agcggccgct tgtacccgaa gcgtatactg acttcatctt tactgcctgg tgtgaacgta
     4021 caggtttcct gggcggcaca ctgatccttc ttctgctttt gagtattccg cttcggggga
     4081 tgctcgtaag tttcgaatcg cgagatcgtt ttgcttccct cctatctgcg ggcatttcat
     4141 tcatgttttt cttccatatc ctgatgaacg ttggcattgc tgtggggctc ctgcctgtca
     4201 cggggttacc cctcagtttc atgagctatg gtggctctca cctcattact gccatggttt
     4261 cccttggaat tttgctcagc gtcgcgcgtc gtcgctttgc gaactgagca ttggatgtcc
     4321 aaagaggcct gttttgggct ggtttgggga tcgtcgaatg ccgaaaatag gagtgtgagt
     4381 tcagagcgtt acgaacagct tcatgacctg ctggctggat atgagtcctc cattctgaag
     4441 ctggagtctg atttgcagga atgccagggc gctctcccgg gcatggaaac aacggcagca
     4501 gagatgactc ctctgttgcc ggatcgcgcc ggtttcgcgc gtgagagatc gattcgcctg
     4561 atgcatcgga cggcgcgtct tcacaaacta tttggagaat ttcgtaaaga attccaaata
     4621 ttgcacagac acatggacaa gattcgtcgg gatgcgcgcc tgacgttgat tcctttgcca
     4681 gaagaaagaa aggaagtgcg caaggcagac tacactttgt ttcgcctggg cgatatgagt
     4741 ttctgtgtca tggaccgtcc tgttcgagta gagcaggatg tttccgtctc aaataagatt
     4801 gcggatgcta cattggatct ctttccgggc agagatacga tttcgatttt tgcacccggg
     4861 aaaagcaggc tcggtctttt gtattttgga gaaggtaaaa ctgaacaggc gatctggttt
     4921 gaagagatgc ttgagcccgt gattgaatcc tctcacatgg tgcagctcca gccggctggc
     4981 gtagcccatc cacgcgtgcg cggaaaattt cgtttccgcg gcgtggacta ctacgttgtg
     5041 accctctccg atacggagta gcgtttaact gttgtaagtc gtcaggaact cgttgaactc
     5101 agcgaggtct tttgcaaact gattcacgcg acctttgccc cattcttttc cgagagcgtt
     5161 gatgagcttg ctttcaccgc cgttcatgga ctgcgcaaaa gtctttcctt tctttgttac
     5221 attcagacgc ttgatgcggc gatcttctcc atctgcctgg cttgtgatca aggaagaatt
     5281 ttggagagca cgcactgcct ggctcatggt cattggatca cgacgcagca tgcgtccaag
     5341 ttcgccctga gaaatgccag gctgtcgttc aacgtgaata agaatagaaa gcgcaagagt
     5401 ggactgtttg tcctttttct gcatattttc gcgcacaatg tccatcgcgc cgcgaaatcc
     5461 ggagcccatt gcaagaatta aatcgttcga agccataaat ccaagaaaac aagaaaggga
     5521 tcagagtcaa tataaatagt ctaaaccact cgcgaagggg aaaaatggta taggtttctc
     5581 aaataaacat tttggagtgt gtggtgaaag atcgcgcaaa catttttgcg acaacagagg
     5641 ccgtcgaatg tttgaatttt cttattgaac ttgcctgcag aatccgcgtg actcggcatc
     5701 attttt
//
LOCUS       DAORFF010000214         5687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7687_length_5687_cov_3.671875, whole genome shotgun sequence.
ACCESSION   DAORFF010000214 DAORFF010000000
VERSION     DAORFF010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7687_length_5687_cov_3.671875"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..995)
                     /locus_tag="PKK76_09810"
     CDS             complement(114..995)
                     /locus_tag="PKK76_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNL68810.1"
                     /translation="MKLEKDALSGGKVYKPSEPFRAKVLVNRPLTSGEEEVRHVVIDI
                     RESDLRYLEGQSLGVLAPGVQENGKPHKLRLYSIASPRGGDADHPGTVSLCVKRLIEK
                     KEDGSIYQGIASNYICDLKPGDDVLVTGPVGMHFLLPADDRTNIIMVATGTGIAPFKS
                     FLDHIFRERSTPWRGTLALFFGAKFKNELIYCNEMDASLRQFESRPHFQHVCAISREE
                     MNAEGGRMYVQHRIDENYDSIRSLLAEDNFALYICGLKGMEKGIEDVFRARLGEEEWE
                     HKKQLYRKDGRWIVEVY"
     gene            complement(1039..2262)
                     /locus_tag="PKK76_09815"
     CDS             complement(1039..2262)
                     /locus_tag="PKK76_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002734844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNL68811.1"
                     /translation="MEILRFLAAGNVDDGKSTLIGRLLYDSNSLQTDLVENIEKASQK
                     LGTEFNLALLTDGLKAEREQGITIDVAYKYFATAKRKFIIADCPGHVQYTRNMVTGAS
                     TADLMILLVDARKGITEQTRRHSAVGSLLGIEHFAVCINKMDLVDYSQSVYDSIREDY
                     QRMAMDLNLRDLKFIPVSASLGENVVHRSVKMPYYDGPSLLEYLETVMPEKDQSQLPF
                     RFPVQNVLLPRDGKMDRYFAGQICAGTIRRGDTVELFPGGKTKIKSIRLMDADLAEAT
                     APQSVSLQIEDEIDVARGCLIASGAEPDAADEWTATVCWMDARALKPGAKYTLRHTTN
                     TLKCQVMSIDYRVDMGTLGKIVTEELAMNDIGRIRIKLARAIRFDPYLANRWTGSFIL
                     IDEANQTAGAGVIEG"
     gene            complement(2286..3176)
                     /gene="cysD"
                     /locus_tag="PKK76_09820"
     CDS             complement(2286..3176)
                     /gene="cysD"
                     /locus_tag="PKK76_09820"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNL68812.1"
                     /translation="MIDHLRQLEAEAIYILRETAAQFERPALLFSGGKDSITLVHLSL
                     KAFRPSRFPYPLVHIDTGHNFPEAITYRDEMAARIDEKLIVRNVQDSINRGTAIEEKG
                     RFASRNTLQTVTLLETIEEFRFDACIGGARRDEEKARAKERIFSVRDSFGQWDPRLQR
                     PELWNIYNGKIQPGENVRVFPLSNWTELDVWNYIRLEQIPIPNLYFTHKRQVFEKDGI
                     LFPVSDFIKLEKEPVSETEVRFRTVGDMTCTAAVASRASSLDEIIEEIKASNTTERGS
                     RLDDKRSESAMEDRKRSGYF"
     gene            complement(3173..4843)
                     /locus_tag="PKK76_09825"
     CDS             complement(3173..4843)
                     /locus_tag="PKK76_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HNL68813.1"
                     /translation="MELSKNEKLKQDSGFLRGSIVEELKNSSASFEKDTTLLLKFHGM
                     YQQDNRDARVKTEEGGTEKAHSLMIRGRIPGGRLNTEQYLVWDELASRVGNGSMRLTT
                     RQSIQLHGILKQDIKRTLQKIHESLLSTTGACGDIVRNVTQSLNFLNDPSLAKLDGVA
                     QLISDHFRARSGAYAEVWLDDEKLPGPVEEPIYGDRYLPRKFKIGVTAAGNNSIDIYT
                     NDMGFAATFENGQINGYFVFAGGGLGRTHRNAKTYARVADNLGWIPASKLIPVAEAIV
                     TIHRDFGDRNDRKHARLKYVVAEKGIDWMRTETESRSGAKFEQKPLPAWNTPEYLGWQ
                     KAKDGTLTLGLHILAGRIVDNAGHSLKTSLRNVIRDYRLSTQVTPDQDLILLGIRESD
                     RPAIEQVFRAAGVRMDAPNSLFSKALACPALPTCSLAIAESERTLPAFLEVIQKKLEK
                     YGLEDRAPTVRMTGCPNGCARPYSAELGFAGRSMNIYGVYVGGDYEGTRITKELSDNV
                     PLSEMEGVIDRLFAMWKEQAPSDRFGDFTHRMPLEELQKALPAVRPAQ"
     gene            4933..5646
                     /locus_tag="PKK76_09830"
     CDS             4933..5646
                     /locus_tag="PKK76_09830"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNL68814.1"
                     /translation="MDLSALNLELAALSTEEVVRKLYALDPAGTVLACSASIEDTVLL
                     HMLAEAAKSAGRKPRAFVLDTGRLPEETYQVLEDCRQKYHFEFEMVFPESQDVEKLLR
                     AKGPFSFYESIENRKECCHIRKVLPLKRALSGATLWITGMRREQSVTRADVPFVELDQ
                     AHGGILKANPLALWTEKMVYDYASAHNVPVHPLHRRGFPSIGCEPCTRAIQPGEDFRA
                     GRWWWENADQKECGLHVKS"
BASE COUNT         1263 a         1424 c         1427 g         1573 t
ORIGIN      
        1 gtcttccggt ttcgcgcaaa tatttgtcag gtcaagggcc tgcgcatcga gcatcgacgg
       61 gagtagcgta agaagagcgc ctaacgtgac gcaagaggct ttcatccaac cagctagtaa
      121 acctctacga tccatcgccc gtcttttcgg tacaactgct tcttgtgttc ccactcttct
      181 tcccccaggc gcgcacggaa aacatcttcg atgcctttct ccatgccctt taagccgcaa
      241 atgtaaagtg caaagttgtc ttcagcaagc aatgaccgaa ttgaatcgta gttttcgtct
      301 attctatgct gaacatacat tctaccgcct tccgcattca tttcttctcg cgaaatcgca
      361 cacacgtgct ggaaatgcgg gcggctttcg aattgccgta agctagcgtc catttcattg
      421 caatagatga gttcgttctt gaatttagca ccaaagaaca atgctaaagt cccgcgccac
      481 ggcgttgacc gttcgcggaa gatatgatcg agaaatgatt tgaaaggtgc gattcccgtg
      541 ccagtggcaa ccataatgat atttgttcga tcgtctgccg gaagcaggaa atgcattcca
      601 acgggcccgg tgacaagcac atcatctcca ggctttagat cgcagatgta gttagatgcg
      661 atgccctgat agatagatcc gtcttctttc ttttctatga gtcgtttgac gcaaagactg
      721 actgtgccgg gatgatctgc gtccccaccg cgaggagatg cgatgctata gaggcgtagc
      781 ttgtgtggtt ttccattctc ctggacgccc ggtgccagaa cccccaggga ttgcccctcg
      841 aggtatcgca gatcactttc gcgtatatca atgacaacat gccgaacttc ttcttcgccg
      901 ctagtaagtg ggcgattgac caggactttt gccctgaaag gttctgatgg cttgtaaact
      961 ttcccgccgg aaagggcgtc cttttccagt ttcattcgac ccaaactgga atacctgcga
     1021 tgaaagtcga gagagaatct agccttctat gactccggct cccgcagtct gatttgcttc
     1081 atcaattaga atgaaagatc ccgtccatcg attcgcaagg taaggatcga agcgaatggc
     1141 gcgcgcgagt ttgattcgaa ttcggccaat atcgttcatt gcaagctctt ctgttacgat
     1201 cttacccagc gttcccatat ccactcgata gtcgatcgac atgacctgac atttgagcgt
     1261 gttggtagta tgcctcaagg tgtattttgc acccggcttg agggctctgg cgtccatcca
     1321 gcaaacagtt gccgtccatt cgtccgctgc gtctggttct gctcccgaag caatcagaca
     1381 acctcttgcc acgtcgatct catcttctat ttgtaaagac acggattgag gggcagtggc
     1441 ttccgcaagg tccgcgtcca tcagtcggat gcttttgatt tttgtttttc cgcccgggaa
     1501 tagttccact gtgtctccgc ggcgaatggt cccggcgcag atttgaccgg caaagtaccg
     1561 atccatttta ccgtcgcgcg gcaatagcac attctgaaca ggaaagcgaa atggtagttg
     1621 cgattgatcc ttctccggca taacagtttc caggtactcg aggagcgagg ggccatcata
     1681 gtatggcatc ttgacggatc gatgaaccac gttttcaccg agtgatgcgc ttactgggat
     1741 gaatttcaag tcgcgcaaat tcaggtccat agccatgcgc tggtagtctt ctcgaatcga
     1801 atcgtagacg gactgactgt agtctacgag atccattttg ttgatgcaca ccgcgaagtg
     1861 ttctatcccg agcagcgatc ctacagcgga atggcgccgc gtttgttctg tgattccttt
     1921 tcgcgcatct actagcaaga tcatcaggtc tgccgtggat gcacctgtga ccatgttgcg
     1981 agtgtattga acgtgtcccg ggcagtctgc gatgatgaat tttcgcttcg cggtcgcaaa
     2041 gtacttatag gccacatcaa ttgtaatgcc ctgttcgcgt tccgctttca atccatctgt
     2101 cagaagtgcg aggttgaatt ccgttccaag cttctggctg gccttttcaa tattctccac
     2161 caggtccgtc tggagagaat tcgaatcata gagcagtctt ccgatcagtg tgcttttgcc
     2221 gtcgtcgaca ttgccggcgg ctaagaatct taaaatttcc atactatttt cctgattgcc
     2281 tggacctaaa aataaccgga gcgctttcga tcttccatgg ctgactcgga tcgtttgtcg
     2341 tcgagtctcg atccacgttc cgttgtgttg ctcgctttaa tctcttcgat gatctcatcc
     2401 agagaagaag cgcgcgaagc cacggcggcc gtgcacgtca tatctccgac agtgcgaaag
     2461 cggacttccg tttctgacac aggctctttt tcgagtttta taaagtctga aacaggaaac
     2521 aggattccat ctttttcaaa gacctgtctt ttgtgcgtga agtaaagatt cggaatcggg
     2581 atttgttcca ggcgaatgta gttccaaacg tcgagttccg tccagttgct aagaggaaac
     2641 acccggacgt tttcaccggg ttggattttt ccattgtata tgttccatag ttctggacgc
     2701 tggagacgcg gatcccattg cccaaacgaa tcgcgcacag agaaaattct ttcttttgcc
     2761 cgggcttttt cttcgtctcg tctggctccg ccaatgcacg catcgaatct gaattcttcg
     2821 atggtttcga gcaaggtcac agtctgtaga gtattgcggc ttgcgaatct gcctttttct
     2881 tcaattgcgg tgccgcgatt gatgctgtcc tgtacatttc tcacgatgag cttctcatca
     2941 attctcgcgg ccatttcgtc gcgatatgtg attgcttcag ggaaattgtg tcctgtgtca
     3001 atatgcacaa gcgggtatgg aaatctagaa gggcgaaacg ctttgagtga aagatgaacc
     3061 agagtgatgg aatcttttcc acccgagaat aaaagcgccg gacgttcgaa ctgggccgca
     3121 gtttcgcgca gaatgtagat tgcttccgct tcgagttgtc tgagatggtc aatcattgtg
     3181 cgggcctcac ggcaggaagt gctttctgca attcttctag aggcatacga tgagtaaagt
     3241 caccgaatcg atcggaagga gcctgctctt tccacatggc gaagagtctg tctatgaccc
     3301 cttccatttc cgagagcgga acattgtctg aaagttcttt cgtaattctg gttccttcgt
     3361 agtcccctcc tacataaact ccataaatgt tcatacttct tccagcaaat cccagttctg
     3421 ccgagtaagg acgcgcacaa ccattcgggc atccagtcat tcgcacggta ggagctcgat
     3481 cctcgagacc gtatttctct aatttcttct gaatgacttc aaggaacgcg ggcaatgtgc
     3541 gttctgactc tgcgatagca agactgcacg ttggaagcgc aggacaggcc agggcttttg
     3601 agaagaggct atttggtgcg tccatacgca cgccagcggc gcgaaacacc tgttcgatgg
     3661 ctggcctgtc agattcacga ataccaagca ggatgagatc ctgatccggg gtgacttgag
     3721 tggagaggcg gtaatcgcga ataacgtttc tcagcgaagt cttcagtgaa tggcccgcat
     3781 tgtctacgat tcttcctgct agaatgtgca gcccgagcgt gagagtgccg tctttcgctt
     3841 tttgccagcc caggtattca ggtgtgttcc atgccggcag tggtttctgt tcgaattttg
     3901 cgccggatct gctttcggtt tccgttcgca tccagtcgat acccttttct gcgaccacgt
     3961 acttcaggcg tgcgtgcttt ctatcgttgc gatctccgaa gtctcgatga atggttacga
     4021 tggcttccgc cacaggaatc aattttgatg cgggaatcca tccgagattg tctgcgactc
     4081 tcgcgtatgt tttcgcattg cgatgagttc ggccaagtcc gcctcccgca aacacaaagt
     4141 atccattgat ttggccattt tcgaaagtgg ccgcgaatcc catatcgttc gtatagatgt
     4201 cgatgctgtt gttgcccgca gccgttacac caattttgaa ttttctcggt aggtaacgat
     4261 ctccatagat tggttcctct acgggccctg gtagtttttc gtcatccagc cagacttctg
     4321 cgtatgcgcc agatcttgct ctgaaatgat cggagatgag ttgagctact ccatccagtt
     4381 tcgcaaggga aggatcgttt agaaagttta acgattgtgt tacattgcga acgatgtcgc
     4441 cgcaggcacc ggtggtggaa aggagcgatt cgtgaatctt ttgtaacgtg cgtttgatgt
     4501 cctgcttgag aatgccatga agctgaatcg actgacgggt ggtaaggcgc atggatccat
     4561 tgcctacgcg tgatgcaagc tcgtcccaga ccaggtattg ctctgtgttc agccttccac
     4621 ccggaattct tccgcggatc atcagagaat gggctttttc tgttccgcct tcttctgttt
     4681 ttacgcgcgc atcgcgattg tcttgctggt acattccgtg gaatttgagc agcaatgttg
     4741 tatctttttc aaaggaagca ctggagtttt tcagctcctc tactatgctg cctcttagga
     4801 agccagagtc ctgcttgagt ttttcgttct tagataattc catggaaatc tctctgtgag
     4861 gccatctttt tcatttgacc ctgacaggac aaacgatttt agtattcata tcaaattact
     4921 aggaaataag ttttggacct ttctgctcta aatttggaac tcgctgccct gtccactgaa
     4981 gaagtggttc ggaagcttta tgccctggac ccggctggca ccgtcctcgc ctgcagtgcg
     5041 agcatagagg acacagtcct tttgcatatg ctggcggagg cggcaaagtc ggcaggaagg
     5101 aaaccgcgtg ccttcgtgct cgatacgggg agattgcccg aagaaaccta ccaggtgctg
     5161 gaagattgca ggcagaagta ccactttgag ttcgaaatgg ttttcccgga atctcaggat
     5221 gtggaaaagc ttttgagggc aaagggccct ttttcctttt acgagagcat tgagaatcgc
     5281 aaggaatgct gtcatattcg caaggttctt ccattaaaga gagcattgtc cggcgccact
     5341 ctctggatta cagggatgag gcgggagcaa tccgtaacgc gggcagatgt tccatttgtg
     5401 gaactggacc aggcgcatgg aggcattctc aaagcaaacc ctctggctct gtggactgag
     5461 aaaatggtct acgactacgc aagtgcgcat aacgttccag ttcatcctct ccatcgtcgc
     5521 ggatttccaa gcattggttg tgagccatgt acgcgggcca tccagcccgg cgaagacttt
     5581 cgcgcaggac gatggtggtg ggaaaacgcg gatcaaaagg aatgcggact tcacgttaag
     5641 tcctgattat gagttcaaaa ctctttcccc tggtgctgga ccttcac
//
LOCUS       DAORFF010000215         5678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7711_length_5678_cov_4.114707, whole genome shotgun sequence.
ACCESSION   DAORFF010000215 DAORFF010000000
VERSION     DAORFF010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7711_length_5678_cov_4.114707"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PKK76_09835"
     CDS             <1..683
                     /locus_tag="PKK76_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68815.1"
                     /translation="KLVPQKTLLIIGIIYKIKVMATNALVKLVLMRFLGNTVMRMSVA
                     YVAVVITGIWNSIVLWRVLRETRLRLSGHMLVRFLTTEIITPERIHSMPPLAREATVR
                     AVANAVVLTQNYHPNMLLLLVRLCEITGIDSPGDYDEWDRFVEILGQLEPEDREFQLD
                     LLSIAAAFDGKLSKFERNYLPQAFGPQTDWYMNRIRVLSRLMVQGRIRAAISHCFEDF
                     NTDVTFTR"
     gene            complement(694..2544)
                     /locus_tag="PKK76_09840"
     CDS             complement(694..2544)
                     /locus_tag="PKK76_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNL68816.1"
                     /translation="MAHSVWQAISMNEFSRKEFLKKTLSWTGKSAAAGIVGGTAYNFF
                     GHSEYKQAFPNIPENHANVQSNGKTVLILGGGLAGIQAACELADRGFQVTVLEKSGHA
                     GGKLKSWKDKKFAQKYFPDGYSREHGLHGVWGFYKNLREFLGRHQIPLNPLGEKESFY
                     SFIDAQGVRNKIKPVKWPIPFDRIEMMNNGIYVPDEKDLKKPAPDLFNAFRATMKLSG
                     FNFKSESQRMYLDSMTFYDWAKKLGVHDQFIRYYYEAMSDMALFMSTKECSALGIANL
                     IRLGATPSETRVDYYKWPPDETVIGPLVKHLEMKGGRVLFHNEVTELVVENGKVTAVK
                     TNQNLPAGSVRRCRICGNLIYGGVEDHCPYCGADASHIEVMTEQEKVPKTFRADHYIL
                     AMDIPGAKHVLMNTDLARQYEYFRNIQNLSQSTILCVNLLYENSDAWERMFPSGTTLS
                     AVDFFPTGFKILGFTSNWSSTGIPGLRQKKVDLIEVQVCRWQQFIGKDFRSIAAAVHA
                     ELKTALPDLPEPTDFYINRWDTYTGYRPGDEKNRPGIQSPIENLMLIGDWVFVPQHSI
                     FMERTNVCAKWVTNLLLEKIGQKEGKIRILESGTPDWQLDLLGLVTSVRV"
     gene            2559..3035
                     /locus_tag="PKK76_09845"
     CDS             2559..3035
                     /locus_tag="PKK76_09845"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNL68817.1"
                     /translation="MKTRFWVLMILIIGSLAAMVIFTGGSKNTSVMLADASHVVDQPS
                     KYQEHELRVRGFVKPGTILRYGDKADFVMTLDGRDLKVHFDGGTQLPDTFTDAAPVRA
                     DGRLAPDTAGLLVANKVEAKCASKYEADYAKKGDFTSARFQRMAADEKVPGGDHPK"
     gene            3032..5539
                     /gene="ccsA"
                     /locus_tag="PKK76_09850"
     CDS             3032..5539
                     /gene="ccsA"
                     /locus_tag="PKK76_09850"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNL68818.1"
                     /translation="MNLNQIGAAFLLLSAALVTFSIFMSLLGMSIRQIRSLIRFLPFE
                     LADDFRKLRGRRRLSHRMSHAVVSVWMVVRRRLIALLGFRSASVALDLARSALYVNAA
                     AASFAMFTLMVLMVQGDLTNHYVVTHSSADLHIFYLITGAWAGSSGSLLFWYFILTSL
                     SAVAVYQSRILRNRMPFLFLVLAGLQFIFVFMGLYFSDAQPFRVFPQPMKAGQGLNPL
                     LQHWGMIIHPPILYVGYVSSAIPFAIAMSVLVSRNTRNAFYPLLRRWTIFSWFFLGTG
                     ILLGSKWAYEELGWGGYWAWDPVENASLMPFLLMTAFLHTAIVSEQRGLLKFWTMLLI
                     LLAYHFSMLGTWITRSGVLQGPHSFSESSIGKPLIIYIGVSFYFYLRYIIMERKRLRP
                     DEELQTITSKEGSMLLNNFLMLLSVFVILIGVFSPLIPLDCRFDDGFSCSKAEWKLTA
                     YNRIMIPVGVLTLFLMGASPLFAWKKSADQVYVKNLRIPLILGVVVTIAFALLYGKMF
                     KPSGEDSAWGSSLVAEVFSILTVGIAVFTIAGIVQEYSRGIRSRRVRLGENSVQAFLH
                     LIGRNKRRYGGYLVHLSVVFLFLGYAGGSFKKTVKMDFMYYRMPQYPGSSEVHYYSGD
                     KSYVDNYEVEAGDLFFAPYFLANADQNNPLHFVVTHEAHYQLREKGDRAVLVERPEGM
                     TPHAFANRAPATNERIMKLLTGFIPGGHMKTERHFHPQIYPVTGEIQRSEDGRANRIP
                     TSKPDMRSSFAEDIYIQLGSIQDARRKTNPDFAHIYEFYYFEFQKSPEAYQQLFPPVI
                     VATLEVWVNPLVKLIWLGSVMFFLSGLILVLPYGERK"
BASE COUNT         1214 a         1413 c         1457 g         1594 t
ORIGIN      
        1 ccaagctcgt gccacagaaa acacttttga tcattgggat catctacaag atcaaagtga
       61 tggcaaccaa tgcacttgtg aagctcgtgc tgatgcgttt tctgggcaac actgtgatgc
      121 gaatgagcgt agcttacgtt gcagtcgtca tcacaggtat ttggaattct atagtattat
      181 ggagagtgct tcgagagact cgtcttcgtc tatcgggcca catgctggtt cgatttttga
      241 ctactgaaat catcaccccc gaacgcatcc attccatgcc tcccctggcc cgcgaagcta
      301 ccgtccgcgc agtggccaat gcggtggttt tgacccagaa ttatcacccc aatatgctcc
      361 ttttgctggt tcggctgtgc gaaatcacgg gaatagacag ccccggtgac tatgacgaat
      421 gggaccgatt tgtggagatc ctggggcagc tggaaccgga agatcgcgag tttcagctgg
      481 atttgttgtc cattgccgct gcttttgatg ggaaattgtc caaattcgaa aggaattacc
      541 tgcctcaggc gtttggccct caaacagact ggtatatgaa tagaattcgt gtcttgagtc
      601 gtttgatggt ccaggggcgg atccgtgctg ccatctccca ttgctttgag gatttcaaca
      661 cggatgtaac cttcacccgt tgaaggttcg cgatcagact cggacgcttg tgaccaggcc
      721 cagaagatcc agttgccagt ctggcgtccc tgattccagg attcggatct ttccttcttt
      781 ttgaccaatc ttctctaata gaagattggt tacccatttc gcgcaaacgt tggttctttc
      841 cataaagatg gaatgctggg gaacaaacac ccagtctccg atcagcatca ggttttcgat
      901 ggggctctgg attccagggc gattcttctc atcccctggt ctgtagcctg tgtaggtgtc
      961 ccagcggtta atatagaagt ctgttggttc gggcagatcg ggcagggccg ttttgagctc
     1021 tgcatgtacg gcagccgcga tggatcgaaa gtcttttcca atgaattgct gccagcgaca
     1081 aacctggacc tcgattagat ccactttctt ttgcctgagg cctggaatgc cagtgctcga
     1141 ccagttgctc gtgaatccaa ggatcttaaa tccggtaggg aagaaatcca cagcactcag
     1201 agttgtgcca gacgggaaca ttctctccca ggcgtcagag ttctcatata gaagatttac
     1261 gcacagaatc gtagactggc tcagattctg gatgtttcta aaatactcgt actgtctggc
     1321 cagatccgtg ttcatgagca cgtgcttggc acctggaatg tccatagcca ggatgtagtg
     1381 gtctgcgcgg aaagtcttgg gcactttctc ctgttctgtc atcacttcaa tgtgagatgc
     1441 gtctgcgccg cagtaaggac aatgatcttc cacgccgccg tagatcaagt tgccacaaat
     1501 tctacatcta cgaacgcttc cggccggcag gttctggttt gtcttcacgg ctgttacctt
     1561 gccgttttct actacaagct ctgtgacttc attgtgaaag agcactctgc cgcccttcat
     1621 ttctagatgt tttaccagag gccctatgac cgtttcatct ggcggccatt tgtaatagtc
     1681 cactctggtt tcgctcggtg tggcgcccag gcgaatgagg ttggcaattc ccagagcgga
     1741 gcattctttc gtgctcatga aaagcgccat atccgacatt gcttcgtagt agtagcgaat
     1801 gaactgatcg tgtacgccca gtttcttggc ccagtcgtag aaggtcatac tgtcgaggta
     1861 cattctctgg ctttcgcttt tgaagttgaa gcctgagagt ttcatcgtgg cgcgaaatgc
     1921 gttgaataga tctggggcag gcttttttag atccttttca tccggcacgt aaattccgtt
     1981 gttcatcatt tcgattcgat cgaacggaat gggccacttg acaggcttga ttttgttgcg
     2041 cactccttgc gcgtctatga agctatagaa ggattccttt tctcccagag ggttgagtgg
     2101 gatctgatgc cttcccagga attcgcgaag atttttatag aagccccaga ctccgtgcag
     2161 cccgtgttcg cgcgagtagc cgtccgggaa gtatttctga gcgaacttct tgtctttcca
     2221 ggacttgagt tttccgcctg catgaccgct tttttcaagt acagttacct ggaagccgcg
     2281 atcggcgagt tcacaggcag cctggatccc cgcgagtcct ccacccagaa tgaggactgt
     2341 ctttccgttt gactgaacat tcgcatgatt ctcaggtatg ttgggaaatg cctgcttgta
     2401 ttccgaatgt ccgaagaagt tgtaggctgt tccacctaca attccggccg cggcgctctt
     2461 gccggtccag gacagtgttt ttttgaggaa ttcttttcgc gaaaactcgt tcatactgat
     2521 tgcttgccat acgctgtgcg ccattttttc ttgcaattat gaaaacccga ttctgggttc
     2581 tgatgattct catcatcggc tcacttgccg caatggtcat ctttaccggc ggaagcaaga
     2641 atacatccgt aatgctcgca gatgcaagcc acgtcgtaga tcagccatcg aagtatcagg
     2701 aacatgaact gagagtgcgc ggatttgtga agccgggtac gatccttcgc tatggagaca
     2761 aagctgattt cgtcatgact ctagacggtc gcgatttgaa ggtgcacttc gatggcggga
     2821 ctcaactccc agacactttc acagatgcag ctccggttcg tgcagacggc cgtcttgcgc
     2881 cagatacggc gggcctactt gtggcaaaca aggtggaagc gaaatgcgca tccaagtatg
     2941 aagcagacta cgcgaagaag ggcgatttta cgagtgctcg ctttcagcgc atggccgcgg
     3001 acgaaaaagt gccgggcgga gatcacccga agtgaatctg aatcaaatcg gtgcggcgtt
     3061 cctacttctc tctgccgctc tagtaacgtt ttctattttc atgtctttgc tcggcatgag
     3121 catccggcaa attcgttctc tcatacgatt tctacctttt gagcttgcag acgactttcg
     3181 caagttgcgc ggaaggcgca ggctgtcgca tcgcatgtct cacgccgttg tgtctgtctg
     3241 gatggtagtg cgtcgcaggc tgattgctct tcttggtttt agaagtgcct cggttgcgct
     3301 ggatcttgct cgatctgctc tctacgtgaa cgctgcggct gcatcctttg ccatgttcac
     3361 cctgatggtg ctgatggtgc agggggacct caccaatcac tatgtagtta cacattcctc
     3421 tgcggatctg cacatcttct acctcatcac tggcgcctgg gccggttcga gcggaagcct
     3481 tttgttctgg tatttcattc tgacttctct cagcgccgtt gcggtttatc agagcagaat
     3541 tctgcgcaat cgcatgccgt ttctctttct ggttcttgct ggattgcagt tcatcttcgt
     3601 gttcatggga ctctacttca gcgacgcgca accctttaga gttttcccgc agccaatgaa
     3661 agcgggtcag ggtctcaatc ctcttctgca gcactggggc atgatcattc atcctcccat
     3721 tctatatgtt gggtacgtaa gctccgcgat tcctttcgcg atcgccatgt ctgttcttgt
     3781 ttcgcgtaac actcgcaatg cgttctatcc acttcttcgc agatggacga tcttctcctg
     3841 gttttttctg ggcacgggga ttctgctggg ttcgaagtgg gcctacgaag aactgggatg
     3901 gggtggctac tgggcctggg atccagtgga gaacgcgagc ctcatgcctt tccttctcat
     3961 gacagcattt cttcacactg cgatcgtatc agaacagcgc ggtcttttga aattctggac
     4021 catgcttttg attcttctcg cgtaccactt tagcatgctg ggcacgtgga tcactcgaag
     4081 cggagtgctc cagggcccgc atagcttttc tgagtcgagc attggcaaac ccctcatcat
     4141 ttacattgga gtgagtttct acttctattt gcgatatatc atcatggaaa ggaagaggct
     4201 tcgaccagat gaggagctgc agaccatcac gtctaaagaa ggaagcatgc ttctcaacaa
     4261 cttcctgatg ttgttgagtg tgtttgtgat tctaatcggg gttttttctc cgctcattcc
     4321 gctggattgc agattcgatg acggtttctc ctgcagcaaa gcagagtgga agctgaccgc
     4381 ctacaatcgc atcatgattc ctgtgggtgt gctcactctg ttcttgatgg gggcctctcc
     4441 gctctttgcg tggaagaaaa gcgcggatca ggtgtacgtg aagaacctca ggatcccgtt
     4501 gatccttggt gtggtggtta caatcgcctt tgctctgtta tacggaaaaa tgttcaagcc
     4561 gtccggtgag gatagcgcct ggggatcgtc tctcgtggcg gaagtctttt ctattcttac
     4621 cgttggaatt gcagttttta cgatagccgg cattgtgcag gaatattctc gcgggatacg
     4681 ttccaggcgt gtcaggctgg gtgaaaactc cgttcaggcg ttcttgcatt tgatcggacg
     4741 aaacaagcga agatacggcg gttatctggt gcatctttct gtggtgtttt tgttcctggg
     4801 atatgcgggc ggatcgttta agaaaacagt caaaatggat ttcatgtatt atagaatgcc
     4861 gcagtaccca ggcagttcgg aagtgcacta ttacagcgga gataaatcat acgtagacaa
     4921 ttacgaagtg gaagctggag atttgttttt tgccccatac tttctggcca atgcggacca
     4981 gaacaatcct cttcattttg tcgtgactca tgaggctcac taccagttgc gcgaaaaagg
     5041 ggacagagcc gttcttgtgg agaggccaga agggatgact cctcacgcct tcgcaaatcg
     5101 cgcgcctgcc acgaatgaaa ggatcatgaa gctccttacc ggttttattc cgggcgggca
     5161 tatgaagaca gaaaggcatt ttcatccgca gatttatccc gtgactggag aaattcagcg
     5221 cagtgaagat ggaagagcga atcgcattcc aaccagtaag ccagacatgc gttcttcttt
     5281 cgcagaagac atctacatcc agcttggatc cattcaggac gcgagacgca aaaccaatcc
     5341 ggactttgcg cacatctatg aattttacta ctttgaattt cagaaaagtc cagaggcata
     5401 ccagcaactc ttcccgcctg tgattgtggc cactctagaa gtgtgggtga atccgctcgt
     5461 gaaattgatc tggctcggtt ctgtaatgtt tttcctttct ggtttgattc tggttctgcc
     5521 gtacggggaa cgcaaatgag cattctgctc acgatcgctc tggttggttg catcctcgcc
     5581 ctcttcttcc ctcttctccg gtcaagatcg gcaccggagt actacgtagg agctcctcag
     5641 gatgagagaa tcgcggaaac ccgcgcgcaa ctccagag
//
LOCUS       DAORFF010000216         5664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7747_length_5664_cov_3.195935, whole genome shotgun sequence.
ACCESSION   DAORFF010000216 DAORFF010000000
VERSION     DAORFF010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7747_length_5664_cov_3.195935"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKK76_09855"
     CDS             complement(<1..358)
                     /locus_tag="PKK76_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68819.1"
                     /translation="MLRARRIALIFPLIAGCLYSCTSEAIKVGDGEIEKPLQDVAGVR
                     REDLASAQSKLDAQGPGSPGLTLPDLYALAVDRTERLAIKQEKVIQAESDRNRAVGSW
                     IPSLSFRETRLGSLPDH"
     gene            478..1488
                     /locus_tag="PKK76_09860"
     CDS             478..1488
                     /locus_tag="PKK76_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68820.1"
                     /translation="MNDRAKRLLEAHKQFEISRLSKDRIEDTISRETSAILDSIGSLT
                     IEQVLSREEIRQYAVHEFQTAPVNQDARSLLTRAGENMLAVVQKSPAKLGELTTREVH
                     DSVVEIVISSRDLRNNLAHDFVQSPLFKKILSDILYQAIRSFLSEDNALTKLPGVSSL
                     FKFGQNLVNQTLPGLDENMGRVIREFIAKNIDNLTRYAESMLTGQLDEKSIRELAEHI
                     WADYKEQKVSKFAVDAGQIKSEDVSAAASLFWEHFRKTELGRDLAGAAVDAFFDHAGK
                     KTIETALSDAGFHRERIVQTLVKGAAPGIVRAVESGAMGKYLDAKLGEFYNSKEAGEI
                     LG"
     gene            complement(1493..1933)
                     /locus_tag="PKK76_09865"
     CDS             complement(1493..1933)
                     /locus_tag="PKK76_09865"
                     /inference="COORDINATES: protein motif:HMM:NF021495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HNL68821.1"
                     /translation="MIPALVSSLHILAFGLGLGSVFMRGIYLRRIAGEGIHRMFMADN
                     VWGVAAIIWIGTGLLRAFGGLEKGTEFYLQSTLFWVKMGLLALTFTLELFPMITFIRW
                     RIQLGRGVKPDTSRALVLARVNDVEIVLVLAILFTASMMARAVI"
     gene            complement(1946..2479)
                     /locus_tag="PKK76_09870"
     CDS             complement(1946..2479)
                     /locus_tag="PKK76_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNL68822.1"
                     /translation="MLKRLLSVGCFPFFLLLGCASAQTMPVVEHVDLPRFMGPWYVIA
                     NIPTFIEKGAHNAVETYSLESDGTIQTVFTFRDGSFEGPEKKYNPTGTVVNKQTNSEW
                     RMQFLWPFKSEFLISYLTPDYSQTVIARTARDYVWIMARTPSIPEKDYQAILEFLSKN
                     GYDIRLIQRVPQQAVAK"
     gene            complement(2542..4647)
                     /locus_tag="PKK76_09875"
     CDS             complement(2542..4647)
                     /locus_tag="PKK76_09875"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL68823.1"
                     /translation="MRTFSKSLLLLAFLAPTILCARAVDLSNHDSVDLAPWVEYLRET
                     PEIAQLAPENINQLAGWQESPDHGLDFGFTDDAYWFRAAVHPRNPGHYVLMVDAACAD
                     LKVVTLNAEGSRTYNLNLDSRGGPDAIRHRRPATEMELPAGDTVILLRLKNEQVLRMP
                     PVIFTARAFYDFRTVEAVVYGAFFGAIVAISLYNFFVYLSMRERAYGIYVFYALMAGA
                     YTAAFQGFHIALFPDAGRYWLLRSTPIFTSLVGFGMNLFAYSFLQMRRHTPRMGIVHL
                     VLAAIAFLLCVAAFLPFVKLSLVLRGIAVAAGVSVSAMFVSGLLVWSRGFAPARYYVL
                     AISLFTLAAMASSLRLIGIIKHSFWIQHGVQAALLAEMILLSLALSDRINLMKVTLEK
                     NLGELAIAHDLVATSEKKYRVLVEDTSDLIFAMDQEGMILSINHAAQRILGYEPRAMA
                     GRSIFSFIFEGGAESQSYDVMIFRDQFEKLGESVLQTRLRFASRHGEPVDLVVRLERI
                     GAIGADSETVVLGKASRHLEDVLLPAILSERGRYSIENFFSFSELVHQRLTRSLGRYF
                     GAETSDDIGLVLREMLINAIEHGNLEITYDEKTKAQAQGKYMDLIRERQQLLPYRERH
                     VLVEYSLNHRRVWFRITDEGPGFDHRAMQAKEEERLRDLSQSHGRGIGLARALFDIVR
                     YNESGNSIVLAKFTKETSK"
     gene            complement(4684..>5664)
                     /locus_tag="PKK76_09880"
     CDS             complement(4684..>5664)
                     /locus_tag="PKK76_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68824.1"
                     /translation="NFPGEFRWDEVPGLQSAAARLMLDRCGIPFDWLADEVKARYGQT
                     VLTDLLIATLAAQAPRGSSMDQLQAWDDVYSQLPSEEKKRVASAFRTAILTDSPVLAL
                     ERAVRYTDLSDPEFLPALLSRMEKIVNSPPHYDTDSASEIILRKLATVKPDEGARLAC
                     RELERERKRGALTFLAGALLAAANGKYNCPVVLSVLEDSSCDAAYYCGEGKICEAKDL
                     ASDVERALVGPPGQKLDPNMRDRALLAAALNVEGSNEILKVWHQRHSYRIDQPSQPAC
                     SKLSLQGQKGESCHCFDKLPASVCGKSRAEAECKFRVNDAERKVDQVVSN"
BASE COUNT         1543 a         1492 c         1381 g         1248 t
ORIGIN      
        1 tgtgatccgg aagagagccc aggcgcgttt cgcgaaatga cagagaagga atccatgatc
       61 ctacggctcg attcctgtca gactctgcct gaatcacttt ttcctgtttg atcgccagac
      121 gttctgtgcg atccaccgca agagcataca gatccggcaa tgtcagccca ggagatccag
      181 gaccctgtgc atcgagtttg ctctgtgcag atgcaagatc ttcccggcgc actccggcaa
      241 cgtcctggag aggcttttca atttcaccat ccccgacctt gattgcttcg gaggtgcacg
      301 aatacaggca acctgcgatg agcggaaaaa tgagagcaat gcggcgggcc cgaagcatac
      361 gaagatttcc tgaatataac cactttttgc gcgcccgcac acatcaaggc aatttgagga
      421 agataaaaca taagatgaaa tccatgacag ggaaaactca ttctggcaaa tggcagcatg
      481 aacgatcgcg caaagcgtct cctcgaagca cacaagcaat ttgaaatctc gcgtctgtca
      541 aaagacagaa tcgaagacac gatatcgcgg gaaaccagtg cgatcctcga ttccattggc
      601 agcctgacca tcgaacaggt cctgagtcgc gaagaaattc ggcagtacgc ggttcacgaa
      661 ttccagacag ctcccgtcaa tcaggacgca cgcagcctcc tcacccgtgc aggagaaaac
      721 atgctggctg tggttcagaa aagcccggca aagctcgggg aattgactac gagagaagta
      781 cacgacagcg tagttgaaat cgtcatctct tcgcgcgatc ttagaaacaa tctggcgcat
      841 gacttcgtgc agagcccatt gtttaagaaa attttgtccg acattctcta ccaggccatc
      901 cgcagctttc taagtgaaga caatgctctt acaaaacttc ctggcgtctc ctctctcttc
      961 aagttcggac aaaacctggt gaaccagact ctgccaggac tggacgaaaa catgggcaga
     1021 gttatacgtg aattcatcgc aaagaacata gacaatctaa cccgttatgc ggaatccatg
     1081 ctcacgggcc agctggacga aaaatcaatt cgagagctgg cagaacatat ctgggcggat
     1141 tacaaagaac aaaaagtgag caagtttgca gtggacgcgg gacaaatcaa gagcgaagac
     1201 gtgagtgcgg ccgcttctct tttctgggag cactttagaa aaacggagct cggaagagat
     1261 ctggccggag cagctgtaga cgctttcttt gatcatgcag gcaaaaaaac aatcgaaact
     1321 gccctctccg acgcaggctt ccaccgcgaa agaatcgttc agactctggt caaaggcgct
     1381 gccccgggaa ttgtgcgcgc agtcgaaagt ggtgctatgg gcaagtatct agacgcgaaa
     1441 cttggagaat tttacaattc aaaagaagcg ggagagatcc tgggttaagc agttagatga
     1501 cggcgcgagc catcatgctg gcagtaaaca gaatcgcaag cacgagtaca atctccacgt
     1561 cattcactcg cgcaagtaca agagcacgag atgtatcggg ctttacaccg cgaccaagct
     1621 ggattcgcca gcgaatgaat gtgatcattg gaaaaagttc gagagtaaag gtgagtgcga
     1681 ggagccccat cttcacccag aaaagagtgc tctgcaaata gaactccgta cctttttcca
     1741 gcccgccaaa ggccctcagt agtccagttc cgatccagat gatggcagcc acgccccaga
     1801 cgttatccgc catgaacatg cgatgaatgc cttcgcctgc gatacggcgc aagtagatgc
     1861 cgcgcatgaa cacactgccc aggcccagac caaaagcaag aatatgaaga gaagaaacta
     1921 acgcgggtat catgatcctg gccgatcatt tcgccacagc ttgctggggc acgcgctgta
     1981 tgaggcgaat gtcatatcca tttttcgaca gaaattcaag aatcgcttga tagtcttttt
     2041 ccggaataga aggagttcgt gccatgatcc agacgtagtc ccgcgcggtt cgcgcgatca
     2101 ctgtctggct gtagtctggg gtcaaatagc tgatgaggaa ttcgcttttg aagggccaga
     2161 ggaactgcat acgccattcc gaattcgttt gtttattcac aaccgtaccc gtaggattgt
     2221 acttcttttc cggtccttca aacgatccgt cgcgaaaggt aaagacagtt tgaatggtgc
     2281 catccgattc aagtgagtaa gtttccaccg cattgtgcgc acccttctca atgaaggtag
     2341 gaatattggc gattacatac cagggcccca taaaacgagg aaggtccacg tgctctacta
     2401 caggcatggt ttgagcactc gcgcaaccca gcagcaagaa gaatggaaaa catccgacag
     2461 agagaaggcg ttttaacatg cgatccaaaa aacttacgtg agcctcgcgc ggcttttctt
     2521 ttttagaaac agacccggga tttacttcga tgtttctttt gtgaactttg ccagaacaat
     2581 ggagttgccg gattcattgt agcgcactat atcaaagagc gctcgcgcca gaccaattcc
     2641 tcttccgtgt gactgagaaa gatctcgaag acgctcctcc tcctttgcct gcatggcgcg
     2701 atgatcaaag cccggacctt catccgtaat gcgaaaccag actctcctgt ggttgagaga
     2761 atactccaca agtacatgtc tttcgcgata gggcaagagc tgttgacgtt cacgaatcag
     2821 atccatgtat ttgccctgag cctgggcttt tgtcttttca tcgtaggtaa tttcaagatt
     2881 gccgtgttca attgcgttga tgagcatctc tcgcagtacc agtccaatgt cgtcactggt
     2941 ttctgcgcca aagtaacgac caagactgcg ggtaagtctc tggtgtacca gctcagaaaa
     3001 actaaaaaag ttttcaatag aatagcgtcc acgttcgctg aggatcgcgg gcagcaacac
     3061 atcttccaga tggcgagaag ctttccccag aacgactgtt tcagagtccg ctccaatggc
     3121 accaattctc tcgagacgca ccacgagatc cacaggctca ccgtgcctcg aggcgaatct
     3181 gagccttgtt tgcagaacac tttcgcccag cttctcaaac tgatcgcgaa agatcatcac
     3241 gtcataggac tgactttcgg caccaccttc aaatatgaat gaaaaaatag aacgtcccgc
     3301 catggcgcgc ggttcgtaac ccagaattct ctgggccgca tgattgatgg aaagaatcat
     3361 tccttcctgg tccatcgcga aaatcaaatc ggatgtatct tccacgagta cgcgatattt
     3421 cttttctgaa gtagccacca gatcgtgtgc gattgcaagc tcccccagat tcttttccag
     3481 agtaaccttc atcaaattga ttcgatcaga aagtgcaaga gagagaagaa tcatctctgc
     3541 aagcaaagcc gcctgaacgc catgctggat ccagaaagag tgtttgatga ttccaatcag
     3601 ccgaagggaa gatgccatgg cagccagagt aaaaagagaa attgccagca cataataacg
     3661 cgcaggtgca aatccgcgac tccatacaag caaaccagaa acaaacatcg cgcttactga
     3721 aacgccggca gccaccgcga tacctctgag cactagagat agtttcacaa atggaaggaa
     3781 tgcggccaca cagagaagaa atgcgatggc cgcaagcacc agatgcacaa tccccattct
     3841 gggcgtatga cgtcgcattt gtaaaaaact gtaagcaaaa aggttcatcc caaaaccaac
     3901 gagactcgtg aagatgggag tagatcgaag cagccagtac ctgcccgcgt ccgggaaaag
     3961 tgcaatatga aaaccctgaa atgctgccgt gtatgcaccg gccatcagag cgtaaaacac
     4021 gtagatcccg tatgctcttt cgcgcatgga tagatagaca aagaaattat aaagcgaaat
     4081 ggcaacgatc gccccgaaga acgcaccgta tacaaccgct tccaccgtgc gaaaatcgta
     4141 aaatgccctg gctgtgaaaa tcaccggagg catacgcaag acctgttcgt ttttcaatcg
     4201 cagtagaatc acagtatccc ctgcaggcag ctccatctct gtggccggcc tgcgatggcg
     4261 aatcgcatct ggaccaccgc gcgaatcaag attcagatta tatgttcttg atccttccgc
     4321 gttcaaggtc actaccttca gatccgcgca agctgcatcc accatcagaa catagtgacc
     4381 gggatttctc ggatgcacgg ctgcgcgaaa ccagtacgcg tcgtctgtaa aaccaaagtc
     4441 caacccatga tcgggagatt cctgccagcc cgcgagctga ttgatgtttt caggcgcaag
     4501 ttgcgctatt tccggagttt cgcggagata ctccacccat ggagccaggt ccacggaatc
     4561 gtgattggac aggtcaaccg ctcgcgcgca aagaatcgtg ggtgcaagaa aagcaagaag
     4621 caaaaggctc ttgctgaaag tacgcacagg cgcgttgatt ttccgttgct ttcgcatgta
     4681 gatttagttc gaaactactt gatctacttt tctttccgca tcattcacgc gaaacttgca
     4741 ctcggcctct gcacgtgatt tcccgcatac agacgcgggc aacttatcaa agcaatgaca
     4801 cgattcccct ttctgtccct gcagcgaaag cttgctgcag gccggttgcg atggctgatc
     4861 gattctgtag gaatgcctct ggtgccatac ctttagtatt tcgttcgatc cttccacatt
     4921 cagagccgcc gcaaggagcg cacgatcgcg catgttagga tcgagttttt gcccgggcgg
     4981 tccgacgagt gcgcgctcaa cgtctgaagc caggtctttt gcttcgcata tctttccttc
     5041 tccgcaatag taagcagcat cgcaggacga gtcctcaaga acggagagaa ccacaggaca
     5101 gttatacttc ccatttgccg ccgcaagaag tgctcccgca aggaacgtca gcgctcccct
     5161 ttttctttct cgctccagtt cacgacaggc cagacgagcg ccttcgtccg gtttcacggt
     5221 ggcgagtttg cgaaggatga tttcagatgc agagtccgtg tcatagtgag gaggggagtt
     5281 gacgatcttc tccatgcgcg aaagaagagc tggtagaaat tcaggatctg acaggtctgt
     5341 ataccgcact gcccgctcca gagcaagcac aggcgaatca gttagaatcg ctgtgcgaaa
     5401 tgcagacgca acacgcttct tttcttccga aggcaattga gaatacacgt catcccatgc
     5461 ctgcagctgg tccatggaac ttccgcgcgg agcctgagcg gctaacgttg caatcaacag
     5521 atccgtcaaa acagtctggc catatcgagc tttcacttca tctgcaagcc aatcgaaagg
     5581 aattccacac cgatccagca tcaatctggc cgcggcgctc tgcagacctg gcacctcatc
     5641 ccagcgaaat tcgccaggaa aatt
//
LOCUS       DAORFF010000217         5650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7784_length_5650_cov_4.106345, whole genome shotgun sequence.
ACCESSION   DAORFF010000217 DAORFF010000000
VERSION     DAORFF010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7784_length_5650_cov_4.106345"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..496)
                     /locus_tag="PKK76_09885"
     CDS             complement(137..496)
                     /locus_tag="PKK76_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68825.1"
                     /translation="MRFAISFALYGAIVFWIFQNHPDHAIALCLHSAFGFVLSGAFDA
                     ARIIVSKTNIKRALTIFFVGVFVRMAAVGIYGMLHMFAGTIVPASALISFAGSIVASL
                     LFSVAWEAGWQIYSLRK"
     gene            complement(496..801)
                     /locus_tag="PKK76_09890"
     CDS             complement(496..801)
                     /locus_tag="PKK76_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNL68826.1"
                     /translation="MQNMGGVIGIGFEFLGIAGFFTAAGIYLDGRFAGRGLILFALVI
                     VGVSAGLWHMIRRVKSIEIEMRAQKKIQKPEKPEDHLRKLDEMTRSLEEMTRKKKDD"
     gene            complement(992..2062)
                     /gene="lepB"
                     /locus_tag="PKK76_09895"
     CDS             complement(992..2062)
                     /gene="lepB"
                     /locus_tag="PKK76_09895"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNL68827.1"
                     /translation="MKSRGESATSFAVSMAVLVFFVFAFKQSVLDANNIPSGSMIPTL
                     KIGDYLFVNKMRYSLRIPFTEVELIRIDDPARGDIITFIPPGDPGKNYVKRVMGMPGD
                     RIRIRSVPACDLYKAYGETLPNVPREFSCDRKSPSYRYEPQLAFVEYKEGDSGEWKNY
                     HPTELGSEEARKLLLDSDNVGVLPPELLPNFDWRGRMPVAFRENLPSGGTHLTIESSY
                     TSPNDSLCEDIETTGCVIPPNQYLAMGDNRDDSRDSRYIGYIAREKILGKVVIIYFSI
                     NWKDDICKEFARDFLRSSVPPALDDGYKIPGFPPEKQRRRCSLLDEQADSESIIDYLR
                     RTVVYRIPRMDVRWTRIANLLK"
     gene            complement(2101..4632)
                     /locus_tag="PKK76_09900"
     CDS             complement(2101..4632)
                     /locus_tag="PKK76_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68828.1"
                     /translation="MRYYVVLMFLLISSALHAVEPDVFVGGKKILFAELKASEWKTLS
                     LRDRRMMAPRLVDYGTFNATRRSEFVELLSAESDPYLRAHAIVARAFFRSADDRIVRD
                     ELQSQLSRADSISRPILEYYAAMLAKMPAANLSSLACKFSELDDSCRAIKLRVYIEQL
                     ALLPQVSVQQIRDLQSMAAPLLLKNPIKPAMYHRIAEGVPEKLSRLRLPLEAAIIQER
                     MLRGIESAWANDLRARIPFYLATAGDFASALRYTENRASLRNIDLRMARLDWMILSGR
                     YKQAVDSLTEIIEGNIDLSGLHGKDPWTGFTYSKEGLRLRLAMVLHLAGDSGRAARAL
                     EMLKDSKGKTDLGEPVALYSRIRLAQILLKDRPELAHKLAEDVTYEAQAQNWSQLEYY
                     ATILDGWALYYSKQYFPAVVNFTKAGGIVRDPPAEYSRLLGLMVSQMAMAPASPQIYS
                     IQRLKTMLASRPYHRAIYTIRDWVPEDAGQDFFIEQSVLNFETRKDRWSSLLLLEEFK
                     RLEEFYFHAGNNPGGARGLLTSDVWFRTLREFPSVRILSRGVLEDNADSLRASIQGLI
                     QPQALFLADDSAYLFPFQVRDKVYTYLVAPAVPRSRAPRLILREDMSVADYFALADSC
                     RRGPCKESFPELGKFAASFRILQVQWRTGDPDFQKLFRRQDLSVYLFYDGFLPPKQYK
                     STYHGAYKPACSTPGAPLDASPLSFDSVLDGVYAGFAIFWPGELDVRVGEDGERRPVY
                     LRNFICGDARVRFWDLDRFWSSPPELLVYQTRQEEPDMDRAFLRFASGAGMVMLESNK
                     LTVEQAETLKGLARQGLSYPVIQDRLRAAYPDAEIRFYFPGPVSK"
     gene            4712..5449
                     /locus_tag="PKK76_09905"
     CDS             4712..5449
                     /locus_tag="PKK76_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68829.1"
                     /translation="MKQMYLVFAFLISAAVSAQATQPGTPASTTPAASQEDPHNADNY
                     LEKQTALKGIDQARSEQQKDIRKLSVIVANYGSAVAGSDAELKKIKDLYEESSVLYYR
                     QQYVKSREILNQAKTQTSELYKKFDDMYAKQTDELMIQCAKGVANLETTQTPGGGNSP
                     EWSTVLILNQSRLRTAFGHVVIAQDLNREKRYDDAIDHYRLAKLFGIGLLRGLEEDPQ
                     KAKAIDDKYKIDLQDATGGRAASAPSK"
BASE COUNT         1493 a         1488 c         1391 g         1278 t
ORIGIN      
        1 gcatccataa gatgatgaac tagaatttct ccaagatcaa acttgtccgc ttcagatgtt
       61 ggcgattctg catgaagcgt tgccgggacg aatgcaattg ccgcggcgaa agtgagcact
      121 cttgtgattt ggcggatcat tttcggagac tatatatttg ccacccagct tcccacgcaa
      181 ccgaaaaaag aagactcgca actatcgatc cggcaaatga aatcaatgca gaagcaggta
      241 cgatggtccc tgcaaacata tgcaacatcc cgtaaatgcc aacggccgcc attcgaacaa
      301 agacaccaac gaagaaaatc gtcagcgccc ttttgatgtt tgttttagat acgatgatgc
      361 gagccgcgtc aaaagcaccg ctcaaaacga atccaaacgc agaatgaagg cagagtgcaa
      421 tcgcatgatc tggatggttt tgaaaaatcc agaatacaat tgcgccatac agagcgaagc
      481 tgatcgcaaa tcgcatcaat cgtccttttt cttgcgcgtc atttcttcga gagatcgagt
      541 catctcatct agcttgcgaa gatgatcttc tggcttctct ggcttctgaa tttttttctg
      601 agcgcgcatc tcaatctcaa tcgacttcac gcgtcggatc atatgccaga gaccggcgga
      661 tactccaaca atcacaagag caaacagaat cagtccacgg cctgcgaatc gtccatcgag
      721 gtaaatgcct gcggcagtga agaatccggc aattcccaga aattcaaagc cgatcccgat
      781 cactccgccc atgttctgca tggaaccgaa atatctgcag caactagcct ggaaagccga
      841 ttccagacct gtggagtcac tcaagctcaa acaagtcgcc catggctggg tactttcaga
      901 cgggaagaaa tatgcaaccg gggcaacccg atccgagacc tggaaagagt gggtggagaa
      961 ccccaggccg ctcccgcgcc cgggttactt tctatttcag aagattggcg attctagtcc
     1021 agcgcacatc cattctgggg atgcgataca cgaccgtccg tctgagataa tcgatgatgg
     1081 attctgagtc cgcctgttca tccaggaggg aacagcgacg acgctgtttt tccgggggaa
     1141 atccggggat cttgtatcca tcgtccaggg caggaggcac ggaagaacgc aggaaatcac
     1201 gtgcaaattc cttgcagatg tcatccttcc agttgatgct gaagtagatg atcaccacct
     1261 tgcccaggat cttctctctc gctatataac caatgtaacg cgaatcgcgc gaatcgtctc
     1321 gattgtctcc catggccaga tactggttag gaggaatgac gcagcctgtc gtttcaatgt
     1381 cttcacacag actgtcgttg ggagacgtat acgagctttc tatggtcaga tgagtgccac
     1441 cggagggcaa attttcgcga aaggcaacag gcattcttcc gcgccagtca aagttaggca
     1501 gcagttcggg agggagcaca cccacattgt ctgaatcgag aagtagcttg cgggcctctt
     1561 ctgatccaag ctcggttggg tgatagttct tccattctcc ggaatcccct tccttgtatt
     1621 ctacaaacgc cagctgtggt tcgtatctat aagaagggga ttttcggtcg caggagaatt
     1681 cacgaggaac attggggaga gtctcaccgt aggctttata tagatcgcag gccggaacgc
     1741 tacgaatgcg aattcgatct ccgggcattc ccatcactct tttcacgtaa ttctttcccg
     1801 ggtctcccgg aggaatgaat gtgatgatgt ctccgcgcgc aggatcatcg atacggatga
     1861 gttccacttc tgtaaaagga atgcgcaaag aataacgcat tttgtttacg aagaggtagt
     1921 ccccgatttt cagagtgggg atcatggaac cgctcgggat gttgtttgcg tctagaactg
     1981 attgcttgaa cgcgaaaacg aagaacacaa gaaccgccat tgagacggcg aatgacgtgg
     2041 cgctttctcc ccgcgatttc actgagtcct tcctttcctc tgccgggcca ggggaaagca
     2101 ttatttgcta accgggccag ggaaatagaa acgaatctcc gcatccggat aagcggcgcg
     2161 gaggcgatct tgaatcactg gataagaaag cccctgacgc gccaggcctt tcaatgtttc
     2221 cgcctgctcc accgtgagct tgttcgattc cagcatcacc ataccggcgc cagatgcgaa
     2281 gcgcagaaac gctctgtcca tgtccggctc ttcctgtctg gtctggtaca cgaggagctc
     2341 tggtggagaa ctccagaatc gatccagatc ccagaagcga acgcgagcat ctccgcaaat
     2401 aaaattgcgc agataaaccg ggcgcctttc tccatcctcc cctaccctca catccagttc
     2461 tccaggccag aagatggcaa accccgcata gactccgtcc agtacagaat caaacgaaag
     2521 cggcgaagcg tctagcggcg caccaggagt ggaacaggca ggcttgtacg ctccgtgata
     2581 ggtactcttg tattgctttg gcggtaaaaa cccgtcgtag aagagataca cagaaagatc
     2641 ctgtctgcga aacagtttct ggaaatcagg atccccggtt ctccactgca cctgcagaat
     2701 ccgaaaagag gccgcaaatt tgccgagctc tggaaacgat tctttgcagg gccctcttct
     2761 gcatgaatca gcaagcgcga aatagtctgc tacgctcatg tcctcgcgca agatgagtct
     2821 gggtgctctg gaccttggaa ctgcaggtgc taccagatag gtatacactt tgtctcgaac
     2881 ttgaaaggga aacagataag ccgagtcatc cgccagaaag agtgcctggg gttgaatgag
     2941 tccctgaatg ctcgcgcgaa ggctgtctgc attgtcttct agaacaccac gcgacaggat
     3001 ccgaacagag ggaaattcac gcaacgtgcg aaaccataca tcggatgtaa gaaggccgcg
     3061 cgctccgcct ggattgttcc ccgcgtgaaa gtaaaactct tccaacctct taaactcttc
     3121 taacaataga agagaagacc atcgatcctt tctcgtttcg aaattcaaaa cagactgctc
     3181 tatgaagaaa tcctgtcccg catcttctgg cacccagtct ctgatggtat agatggcccg
     3241 gtgatacgga cgagaagcga gcatcgtctt gagacgctgg atagaataga tctgaggcga
     3301 agcgggcgcc atggccatct gcgaaaccat tagccccagc agtcgcgaat actctgcggg
     3361 cggatcgcgc acgattccac ctgcctttgt aaaatttacg accgcaggaa agtattgctt
     3421 actataatag agtgcccagc cgtccagaat cgtagcgtag tattctagct gagaccagtt
     3481 ctgtgcctgc gcttcgtagg taacgtcttc tgccagctta tgagcaagct cgggcctatc
     3541 tttcaacaga atttgcgcga gccgaatgcg cgaataaagt gcaacaggct ccccgagatc
     3601 cgtcttccct ttggagtctt tgagcatttc cagggcgcgg gcggcgcgtc cgctgtctcc
     3661 agccagatga agcaccatgg ccaatcgcaa acgcaatcct tctttggaat atgtgaagcc
     3721 agtccacgga tccttcccgt gtagaccgct caaatcaatg tttccttcta tgatttcagt
     3781 gaggctatcc actgcctgtt tgtatctacc ggatagaatc atccagtcca gtctggccat
     3841 gcgaaggtca atgttgcgaa gtgacgcgcg attctccgtg taccgaagag cggacgcgaa
     3901 atcgccagca gtggccagat aaaaaggaat gcgagcgcgc aaatcattcg cccatgcact
     3961 ttcaattccg cgaagcattc tctcctggat gatggcggct tccagtggaa ggcgaagcct
     4021 gctgagtttt tctggaacgc cctccgcaat gcgatggtac atggcgggct tgattggatt
     4081 ctttagcaga agaggcgcgg ccatcgactg cagatcacga atctgttgca cactgacctg
     4141 aggcaacaat gcaagctgct cgatgtagac gcgaagcttg atcgctctac aactgtcatc
     4201 cagttccgaa aacttacatg cgagagaaga gagattggca gcaggcattt ttgcaagcat
     4261 ggctgcatag tattccagaa tcggtcttga gatggaatct gctctggata gttgtgactg
     4321 caactcatct cgcacgatcc gatcatctgc gcttctaaag aaagcacgtg cgacgattgc
     4381 atgggcacgg agatatggat ccgattcagc ggaaagaagt tcaacaaatt cgcttctgcg
     4441 ggtcgcattg aatgttccat agtcaacgag acgaggcgcc atcatcctgc gatcacgaag
     4501 agaaagagtt ttccactccg atgccttcag ctcagcaaat aagatctttt tgccgccgac
     4561 aaacacatcc ggctccacag catgaagagc actcgaaata agaaggaaca tcaagacgac
     4621 ataatatcgc acaggacaga atcagctcac gagtccgaac tgtaaaacgt ttttggtttg
     4681 acgaggaaac aagcgggcac atcacaacag catgaaacaa atgtatctgg tcttcgcatt
     4741 tctcatctca gccgcagtat ccgctcaagc tacgcagccg ggaacgcccg catccactac
     4801 tccagcggcg tcacaggaag accctcataa cgcagacaat tacctcgaaa agcaaacggc
     4861 cctcaaaggc attgatcagg caagatcaga gcagcagaag gatattcgca agctttctgt
     4921 gatcgttgca aactacggtt cagcggtggc aggctcagat gcagaactaa agaaaattaa
     4981 ggatctctat gaagagtcat ccgttctcta ctaccgccag cagtatgtaa agtcccgcga
     5041 aatcttaaac caggcaaaaa cacagacatc tgaactctac aagaagttcg atgacatgta
     5101 tgcaaaacaa acggacgaac tcatgattca gtgcgccaaa ggagtagcca atctcgaaac
     5161 aactcaaact ccgggtggcg gaaactctcc tgaatggtcc acagtgttga ttctcaatca
     5221 atctcgtctg cgcactgctt ttggtcatgt agtcattgcc caggacctca atcgtgaaaa
     5281 acgctatgat gatgcaatcg atcactacag acttgcgaaa ttgtttggaa ttggtttgct
     5341 tcgcggactg gaagaagatc cccagaaagc aaaagcaatc gacgacaaat acaagataga
     5401 tttgcaggac gcaactggcg gcagagctgc ctcagctcct tctaagtaaa atcccttgac
     5461 ggcgcgcccc gcgccgtctg tgtcaactca gtgcatcgcc ccatcgtctt ctttctcgca
     5521 tccttctttg caggatgcct tccactcttc gctttcccgg atgaaattcc agtcgtcatg
     5581 gaccctgggc acggcggtgc cattgtcccg cgcaaggatg acaaatggga ccctgttacg
     5641 cgtcagtatc
//
LOCUS       DAORFF010000218         5642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7803_length_5642_cov_3.624843, whole genome shotgun sequence.
ACCESSION   DAORFF010000218 DAORFF010000000
VERSION     DAORFF010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7803_length_5642_cov_3.624843"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PKK76_09910"
     CDS             complement(<1..769)
                     /locus_tag="PKK76_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNL68830.1"
                     /translation="MAQKKAKKTGRKHVEKVIAEDVVSLPLQIVPVRRDFRLTKIICT
                     IGPASEATTMLEKLARAGMNVARINMSHGTREKHREIIRRIKNLNRKLNHPIAILMDL
                     QGPEIRTGELKKNINLAVGEIFTFNILPEENPEERSVFVNYKGMVDQLKAGDRITVDN
                     GLINLQVLEKSLTGLRCVVLEGGTLGSRKHINLPGVRVNLPSLAEKDKRDIEMALQED
                     LDFIALSFVRSADAVTEARAMVESSGGHTRIISKIENE"
     gene            complement(795..2693)
                     /locus_tag="PKK76_09915"
     CDS             complement(795..2693)
                     /locus_tag="PKK76_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68831.1"
                     /translation="MAVTKQQADSATGQPFEVLDLQQSTEFVQALEARGDFKFKGIFK
                     VFQTILDWTGRFSSTNILPSSDQIAKDVEIVQDRLNIYLLELQGRGSSKEKFVEAIPY
                     IDLSTEGLVPMVVFARPTKQDAGSARRYTDAYRDKSLASIKKYLAYRRPLQYDDFEKL
                     IRTYVESGKLAELQAGSEISRLFFQDPDPNVRKKYHTHYVIPIYRKLVEEKLFVALPE
                     LKPRILLYNVSDELWNRFFILADILLSSLGESGKFSDRPDPRELGSYLNGYDPGRVEG
                     MRPGYKQAFQELVLLCGLLEKRTEEERERKKKEELQKLSDEIGKLPRVVELRKFERYP
                     EEVRNQLLRSGSVLQAEVPVGSRMGHFVLHKQRVKDAVKNARDAFDQTGDPVEVRILS
                     AMSVEDFLTGDEVRAFHDLEQRTYMEHLPWLVRVWRALFGNAKLKPEEIKKIKQKVDR
                     EQQEASLRIRKQEADKSKRELAAKKLKGEDGKPEAAPAKPREEETVTTEEDLVHKNVV
                     MDEKIQETVRGMVTELDHAWDKNMLPNRNFLIEKYPIFNEETIINFLKKYGRTEILSF
                     RIMHEKPEYLWPILISRRYVKAKGKAMLAKAKHDADEQRKAMMPNQEKFDIATALEDF
                     LNRILPKM"
     gene            2708..3463
                     /locus_tag="PKK76_09920"
     CDS             2708..3463
                     /locus_tag="PKK76_09920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HNL68832.1"
                     /translation="MQETTPLTSRTKLPSAAEKSDYVRTNFDAIARNYDRFNDWISFG
                     MHRLWKKRMVQETGLTGKKARVLDLCCGSGDISLAFARILADGSEITSLDFSHEMLNV
                     LSHRLLTFPSRGVQIRPLEGDAMKLNYSSEFDAASMSFGLRNVTDRALCLRGILRALK
                     PGAPFVLLDVGKPPSIFMPFFRVFFEGIVPKIGHWIHGGKHSMYDYLPASARAYPDQK
                     AMKKELEDAGFVNVRIINFSLGAAGLHVARKPE"
     gene            3460..4389
                     /locus_tag="PKK76_09925"
     CDS             3460..4389
                     /locus_tag="PKK76_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68833.1"
                     /translation="MMKDFLIAVKRTKWERDLRTYGSEEKIRQIYQIQNNAYDRVFAS
                     HQRQKESFELVKQTLKNSDFFFREDLQSASFENYRCIASLGGDNHFVYVSRFAKSPIA
                     GINSDPKSSRGALLAGNASEFVQFYSQFDSTPEEDKASFLQKRITERWSRIECTIIFP
                     NGDAQNIGACTSEFSIRNRFAEYMSRYLIRKRLAHSEEGAPPFKEEKCSGLLCATGAG
                     STGWFKSCLPVEERNHAVFSRSAPFFEYLAREALQPMPPARVQSDEALEIISEMDGEI
                     VADSDHELSFSFPYGARAILKLAGERLSVIHEF"
     gene            4389..5384
                     /locus_tag="PKK76_09930"
     CDS             4389..5384
                     /locus_tag="PKK76_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNL68834.1"
                     /translation="MPKEITHIILAEQMREKLPRDVGELLHSHAQEYFFGSTAPDLFY
                     YDVPTPFDASPSSEIISETLHGRMGNKNNEHIFWMLDRMKKLDQQDRYFAFIAGYLSH
                     IAADTIFHPMVYSRTGNYFDQDPAERKRSRARHRVLETSLDLYLLSKSGRTLRGLGLL
                     RLLRVPEDFSRSLMNFFSLSLSEIYHPDLAVSRGALRSVLKSRLIIAMTLSPIAYKFF
                     SWINRILSRKHNDLINLFYPPKPAAIDFETSAQIPHPITGNPYPSRIEELMSMATSRG
                     IAYIAAASAYWKGTISDTELRAAIPPLSLNSGMQCTTVESMIHYEILPGIEQFDD"
     gene            5427..>5642
                     /locus_tag="PKK76_09935"
     CDS             5427..>5642
                     /locus_tag="PKK76_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68835.1"
                     /translation="MNRIVFFALICSLFALFSCRDEKQAVPEKETHQTTVTAGAKGDR
                     LTIAVSVPAGHHAYLDSGKEGVLIPISI"
BASE COUNT         1396 a         1465 c         1299 g         1482 t
ORIGIN      
        1 cttcgttttc aatcttggaa atgattctgg tgtgaccgcc gctcgattca accatcgcac
       61 gcgcttctgt aaccgcatcc gcgcttcgca caaatgagag agcaataaaa tcgagatctt
      121 cctgcagagc catttcaatg tcgcgcttgt ctttctccgc gagagatggc aggttcaccc
      181 tcaccccggg aagattgata tgcttgcgag aaccaagtgt gccgccttcg agcacaacac
      241 agcgaaggcc ggtaagagat ttctcgagaa cctgcaggtt gatcaggcca ttgtcgaccg
      301 tgatgcgatc tcccgctttc agctggtcca ccataccttt atagtttaca aaaacacttc
      361 tttcttccgg attctcttct ggcaaaatgt taaaggtaaa gatctccccc acggccaggt
      421 tgatattctt cttaagttca cccgtgcgaa tctccggtcc ctgcaaatcc atcagtatgg
      481 cgatggggtg attcaatttt cgattcaggt tcttaattct gcggatgatt tctctatgct
      541 tctcgcgtgt gccgtgagac atattgatgc gcgccacgtt cattcctgcg cgagccagct
      601 tctcaagcat cgttgttgct tcggaggccg ggccaatcgt gcagataatt ttggtcaggc
      661 gaaaatcgcg tctgaccgga acgatttgca atggcagaga aacaacatcc tcagcgatca
      721 ctttttcgac atgctttcgc cctgtctttt ttgctttttt ctgtgccatt gatttagtcc
      781 gtggggaacg ttgattacat tttcggcaaa atgcggttta gaaaatcttc gagcgctgtc
      841 gcgatgtcaa atttctcctg atttggcatc atcgcttttc tctgttcgtc tgcatcatgc
      901 ttggccttcg caagcattgc cttccctttt gcctttacat atctacgcga aatgagaatg
      961 ggccagaggt attcgggctt ctcgtgcatg atgcggaacg aaaggatttc agtccttccg
     1021 tacttcttca ggaagttgat gatagtttct tcgttgaaga taggatattt ttcaatcaag
     1081 aaattacgat ttgggagcat gttcttgtcc caggcatgat cgagctctgt aaccattccg
     1141 cgcactgtct cctggatctt ttcatccatg accacgttct tgtgcaccag atcttcttct
     1201 gtagtgacag tttcctcttc gcgcggctta gccggagctg cttctggctt accgtcttct
     1261 cctttcagct ttttggccgc cagctcacgc ttgctcttat ccgcttcctg tttacgaatg
     1321 cgaagacttg cttcttgctg ctcacgatcc actttctgtt tgatcttttt gatttcttcc
     1381 ggcttgagct tagcgtttcc aaaaagagca cgccatactc tcacgagcca gggcaaatgc
     1441 tccatatagg tgcgctgctc taagtcatgg aatgcacgca cctcatctcc ggtaagaaaa
     1501 tcttccacag acatagcaga aagaattctc acttccaccg gatctccggt ctgatcaaat
     1561 gcgtcgcgcg cattcttgac tgcgtctttc acgcgttgct tgtgaagtac aaagtgtccc
     1621 atgcgagaac caacggggac ttcagcctgc agcactgagc ctgaacgaag aagctgattg
     1681 cgaacttctt ccggatatct ttcgaatttt cgaagctcca ccacgcgcgg cagctttcca
     1741 atttcatcag atagtttctg aagttcttcc ttcttcttgc gctctctttc ttcttccgta
     1801 cgtttttcaa gaagaccgca gagaagaacc agttcctgaa atgcctgctt gtagcccgga
     1861 cgcattccct ctactcgccc aggatcgtaa ccgttgagat aactgccgag ctctcttggg
     1921 tccggtctgt ccgaaaattt gccggattca cccagagacg aaagtagaat gtccgcgaga
     1981 atgaaaaagc gattccagag ttcatcgctt acgttgtaga gcagaatgcg cggcttgagt
     2041 tcgggaagag cgacgaatag cttttcttcc acaagctttc tgtaaatggg aattacgtaa
     2101 tgagtgtgat acttctttcg aacgtttgga tctggatcct gaaagaagag acgagagatc
     2161 tctgatcctg cctgcagttc cgccagcttg ccggattcta cataggttcg aatcaatttt
     2221 tcaaaatcgt catattgaag aggacgtctg taagccaggt acttcttgat agaggcaaga
     2281 ctcttgtcgc gatacgcatc cgtatagcga cgagcagatc ccgcatcctg cttcgttgga
     2341 cgcgcgaaaa cgaccatggg cacgaggcct tctgtagaca ggtcaatgta aggaatcgcc
     2401 tcaacaaatt tttcctttga cgacccgcgg ccctggagtt caagaagata gatgttgagt
     2461 ctgtcctgga cgatctcaac atctttggcg atttgatcgg atgaaggcag gatgttcgtg
     2521 gacgaaaagc ggccagtcca gtccagaatg gtctggaata ctttgaagat tcctttgaac
     2581 ttgaagtcac cgcgagcctc tagtgcctgg acgaactccg tagattgctg gagatccaga
     2641 acctcaaaag gctggccggt ggcgctgtct gcctgctgct tggtcaccgc cattggatga
     2701 gccgagaatg caagaaacca ctcccctgac aagcagaaca aaactacctt ccgcggctga
     2761 aaagtcggac tatgtccgaa caaattttga cgccattgcc cgcaattacg accggttcaa
     2821 cgattggatc agctttggca tgcacagact ctggaaaaag cgaatggtcc aggaaacagg
     2881 ccttaccgga aaaaaggcgc gtgtactcga tctatgctgc ggaagcgggg acatcagcct
     2941 ggcgttcgca cgaatcctgg cagacggttc agaaatcacg agtctggact tcagccacga
     3001 aatgctaaac gtcctttcgc acaggctttt aacttttcca tcgcgcggtg tgcaaattcg
     3061 gccgctggaa ggcgatgcga tgaagctcaa ctattcgagc gaattcgatg ccgcaagcat
     3121 gagctttgga cttcgaaatg ttaccgaccg cgccctgtgt ctaagaggaa ttctacgggc
     3181 actgaaacct ggtgcgcctt tcgttctcct cgatgtagga aaaccacctt ccattttcat
     3241 gcctttcttt cgtgtcttct ttgagggaat tgttccgaag atcggccact ggattcacgg
     3301 cggaaagcat tctatgtatg actaccttcc ggcatctgcg cgagcctatc cggatcaaaa
     3361 ggcaatgaag aaagaactag aagacgccgg atttgtgaat gtgcgaatca tcaatttctc
     3421 gctgggcgct gccggccttc acgttgcacg caaaccagaa tgatgaaaga ctttctcatc
     3481 gcagtcaagc gtacaaaatg ggagcgcgat ttgcgcacgt acggttctga ggagaaaatt
     3541 agacagatct atcagatcca gaacaacgct tacgatcgag tatttgcatc tcatcaaaga
     3601 caaaaagaat ctttcgagct ggtcaaacaa actctgaaga attcagactt tttttttcgc
     3661 gaagatttac aatctgcttc tttcgaaaac tacagatgca ttgcctcact tggcggagat
     3721 aatcactttg tgtacgtaag cagattcgct aaaagtccaa tcgcaggaat caattcagat
     3781 cctaaaagct cacgcggcgc acttctggca gggaatgcat ctgaatttgt tcagttttac
     3841 tcacagttcg actccacgcc cgaggaagac aaagcctcct ttctgcaaaa gcgcataacg
     3901 gaacgatgga gtcgcattga atgcaccatc atctttccaa atggagacgc acagaacatt
     3961 ggagcctgca caagcgaatt tagcatcaga aatcgctttg cagagtacat gagcagatac
     4021 ctgatccgaa agagactggc ccattcagaa gaaggagcac ctccgttcaa agaagagaaa
     4081 tgctccgggc tactctgcgc cacaggggct ggatccaccg gttggttcaa aagctgtcta
     4141 ccggtagaag aaaggaatca tgctgtgttc tcaagaagcg caccgttctt tgaatacctg
     4201 gcgcgcgaag cacttcaacc catgcctccc gcgcgcgtcc aatcggatga ggctctagaa
     4261 atcatttcag aaatggacgg cgaaatagtt gccgactcag atcatgaact ttccttctct
     4321 ttcccctatg gagctcgcgc catcctgaag ctcgcggggg aaagactttc ggtcatccat
     4381 gaattctgat gcctaaagaa atcacgcaca tcattctggc agagcaaatg cgagagaagc
     4441 taccgcgcga cgtcggcgag ctcctacatt ctcatgcaca ggaatatttc ttcggcagca
     4501 ccgcacccga tctcttctac tatgacgttc ccactccctt tgacgcaagc ccgtcgtcag
     4561 aaatcatttc agaaactctg cacggaagaa tgggaaacaa aaacaacgag cacatcttct
     4621 ggatgctgga tcgaatgaag aaactggacc agcaggatcg ctacttcgcg ttcattgcag
     4681 ggtatctttc tcacattgcg gcagacacaa tctttcatcc catggtatac agcagaacag
     4741 gaaactactt tgaccaggat ccagcggaaa gaaagcgctc cagagcgcga cacagggtgc
     4801 tcgaaacgtc gcttgatctc tatttgcttt ccaaatcagg aagaacactt cgtggccttg
     4861 gtctgctcag gctactgaga gtccctgaag atttttcccg cagtctcatg aatttctttt
     4921 cgctttctct ttcggaaatc tatcacccgg atctggctgt ttccagaggt gctttgcgct
     4981 ctgttctcaa atcgcgcctc atcattgcca tgactctttc gcctatcgcg tacaaattct
     5041 tttcctggat caaccgcatt ctttcacgca aacacaatga tctaatcaac ctcttctatc
     5101 ctccgaagcc tgcggccatt gactttgaaa cgtccgcaca gattccccat ccgatcaccg
     5161 ggaatcctta tccttccaga attgaagaac tcatgtccat ggctacatcg cgaggcatcg
     5221 catacattgc agcggccagc gcgtactgga agggcacgat ttcagacaca gagcttcgtg
     5281 ccgcgattcc accgctgtct ctcaacagcg gaatgcagtg cacaaccgtg gagagcatga
     5341 ttcactacga aattttacca ggaatagaac agttcgacga ttgagttttt tgttgcctca
     5401 aaggatcaaa tcgtttttct gaaagaatga accggatcgt atttttcgct ctcatttgtt
     5461 cactcttcgc gctcttttcc tgtagagacg aaaaacaggc agtgcctgaa aaagaaactc
     5521 accagactac agtcacagcc ggagcaaaag gagacaggct taccatcgca gtgtctgtgc
     5581 cggccggtca tcatgcgtat ctcgattctg gcaaagaagg agtattgatt cctatttcca
     5641 tc
//
LOCUS       DAORFF010000219         5633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7825_length_5633_cov_4.143241, whole genome shotgun sequence.
ACCESSION   DAORFF010000219 DAORFF010000000
VERSION     DAORFF010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7825_length_5633_cov_4.143241"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PKK76_09940"
     CDS             complement(<1..969)
                     /locus_tag="PKK76_09940"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNL68836.1"
                     /translation="MRVEQIQPGDVSTLTGAHLVLVFGNLDALKLPGFRASLRSKYPN
                     AHIVGCSTAGEIAGDHVRDETVHLTAVEFSNTRVQSAFVHLPSHGSSYSAARSLAMAI
                     PSRDLRHAIVFSEGIRINGSELSRGLKDGLPPNVSVTGGLAADGDRFGQTWVLSNDAE
                     GPDHAVIVGLYGPSLRVGFGSLGGWDSFGPERRITSSSGNRLTGLDGESALALYKKYL
                     GEHASSLPSSALLFPLAIRAEGDDRFLVRTILGIDESDQSLIFAGDMPQGWLARLMKA
                     NFDRLIDGAEGAATTASRRIGNTSPELALLISCVGRKLVLKQRVEEE"
     gene            complement(1045..1941)
                     /locus_tag="PKK76_09945"
     CDS             complement(1045..1941)
                     /locus_tag="PKK76_09945"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNL68837.1"
                     /translation="MKWIGLLIIGLHTFCARPPEVDSHVAEIAREAQQLKAEIAETEE
                     CKSGCIFLSAWDFDGTILKGDMAEGLKEGERVVYPGLQQLAVDQGWSRTYRPGSFQKF
                     LADRSVIEEKEGHLAALSMGLKIFRGVPVMRLESLASSHFEKVLSRYYFRSSISILEN
                     TKKAGIVPCILSASPHVFARSAAATVGVPRENIYAMETEIVRGFMTDKIVEPVPYAKG
                     KAQRLEMIVENLQKTTGRRVFVVAAFGNSFRSDSYFLKWTLDQKLPRGHALAVMINGG
                     PDAGEFRGLFREVSQIATVSGR"
     gene            2037..2885
                     /locus_tag="PKK76_09950"
     CDS             2037..2885
                     /locus_tag="PKK76_09950"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNL68838.1"
                     /translation="MYSHQESTFKSSKDDFPIYYQKWTPARTRRIVVIQHGFGEHSGR
                     YQNIRDAFEGTDTAFYGLDSRGHGKTGGKRGHVPQFQLYVDDLADLIQLVRKENMNDE
                     VYLLGHSLGGVIVLQYALQGTNQSNLSALIVSSPGLIIEMDASKQVKKVMATLFASVA
                     PAATLEAGLDLKYLSHDQAVVDAYKADPLVHGKISFQMGSNLFHLGKTIFEKANHLTI
                     PVYVFQGTEDKIVSPESARQLYKSLTTEDRTLNMYEGLYHETMNETLADRNKVLGDLR
                     AWVEKH"
     gene            2872..3654
                     /locus_tag="PKK76_09955"
     CDS             2872..3654
                     /locus_tag="PKK76_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNL68839.1"
                     /translation="MKNTDPAGALLRFQEVGQGHPLIILHGLFGSGRNWGSVARSLSE
                     KFRVFSLDQRNHGDSPQFPSHTLDDLANDLGEWIESHMKDVPVLLGHSMGGIAAMEYT
                     LRHPDQVRALIVVDVAPRPYEPRHSGEFQAMEADLSSCQTRSDVDRVMAPFVPDLTVR
                     QFLQTNLIRKDHGFGWRIPVETLKKASFLQGFEPGRSWNGPTLFITGSQSDYTSESDH
                     TVMRQCFPEAYIETIEGAGHWPHFSHLVPFLGLIKDFLGRAL"
     gene            3712..>5633
                     /locus_tag="PKK76_09960"
     CDS             3712..>5633
                     /locus_tag="PKK76_09960"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL68840.1"
                     /translation="MARLFAAMVLSLPATLWATPLLRTAEPVIPEMQILEDRANLTPE
                     KAAASSFTVAPEHLSFGYTDSVTWLKFDLENQTSEPHWLIYISYPLLDRVTLYTREDG
                     SLRAHESGQLVSMSAKDYASRYIVFRITIEPGSRQSYLLRVQSADSVVIPLQVMTFES
                     LASREHNEQIAVGVFYGIVILLILYNLLIFFYAKESSYLFYVYYVTSYLLMQLSENGI
                     LGEMLLPEVPWFANQLLAFLVYHVIFAASAFSYRFLRAERFKALSYILRVFGFLGVVG
                     MCATFFVPYMIAVMPSVIIVMSFGPIVVPLAILDWRRGNRAAGHFVLAWSAILAGGFV
                     YGLKAFGILPDMFITRYGAMLGFCVQSVLLSLGLADGIKVLNLELSQLKTSLELRTRY
                     LENIISHAKEASSSLIDANQEETALVQGLVSMADSQADLTHQLSSAFEELSASSESIH
                     GITTRLLENWQETRTTISALSSVQETVLSSGRAVLENVLEISQSSDESGKRLDQMQDQ
                     MKVIQEGGRAISRFAKTISDITDKTNLLALNAAIEAARAGEHGRGFAVVADEIAKLAE
                     ATQKSSKEIFKQIEGIQKDIEGGMDSASAMREALGAVFTRLQKVRASIDDVNASMSAQ
                     EDSMQGLLRQTAQLEN"
BASE COUNT         1403 a         1547 c         1374 g         1309 t
ORIGIN      
        1 ttcctcctct accctttgtt tgaggacaag cttgcgtcca acgcagctaa tcaacagtgc
       61 aagctcgggg gatgtatttc ctattcgacg cgacgcagtc gtagcggcgc cctccgcacc
      121 atcaatcaag cgatcgaaat tcgccttcat caatcgcgcc agccatccct gaggcatgtc
      181 tccagcaaaa atcaggctct ggtcgctttc atcaattccg aggatagtgc gaaccaggaa
      241 acgatcatct ccttctgcgc gaatcgcaag aggaaaaaga agtgcgctcg aagggagaga
      301 cgatgcatgc tcccctaaat actttttgta cagtgcaagg gcagactccc cgtccaggcc
      361 cgtgagtcga ttgcctgagg aagacgttat gcggcgttcc gggccaaagg aatcccagcc
      421 tccgagtgaa ccaaaaccaa cgcgaagaga agggccatat agaccaacaa ttacagcgtg
      481 atcgggacct tctgcgtcat tggacaaaac ccaggtctgt ccgaatcgat ctccgtctgc
      541 agccagccca ccggttacgg acacgttagg tggtaatcca tccttgagcc cccgcgaaag
      601 ctcgctaccg ttgattcgga tcccttccga aaagacaatc gcgtggcgta gatcccggga
      661 tgggattgcc atggccagag agcgcgctgc agaataactg gacccgtgag acggaagatg
      721 aacgaaagca gattgaactc gggtattgga aaactcaacg gctgtcaggt gcactgtttc
      781 atctcgcaca tgatcccctg caatttcgcc tgcggtggaa cagcctacga tgtgcgcgtt
      841 cgggtatttg ctacgcaaag aagcgcgaaa gccaggcagt ttcaacgcat ccaggttccc
      901 gaataccaga accagatgcg cgcctgtgag ggtgctgaca tcgcctggct ggatctgctc
      961 gacgcgcata ggggcaaccg aactctccct tttgttttta gtaaagccta gtttgcccgc
     1021 cggaggacct gtcgcgggga aattttatct acctgataca gtggcgatct gcgaaacttc
     1081 gcgaaacaac ccccgaaact ctcccgcatc tggaccgcca ttgatcatca cagccagggc
     1141 gtggccacgc ggcagcttct ggtccagggt ccacttgaga aaataggaat cagatcgaaa
     1201 gctattgccg aatgcggcca ccacaaagac ccgacgcccc gtcgtcttct gtagattttc
     1261 tacaatcatc tccagccgct gagcctttcc tttcgcgtaa ggtacaggtt cgacaatttt
     1321 atccgtcata aatccccgca cgatctccgt ctccatggcg tagatgtttt cgcgcggaac
     1381 tccgaccgtt gctgcggcgg aacgcgcaaa aacgtgaggc gatgcagaaa ggatgcacgg
     1441 cacaatgcca gccttcttcg tgttttcaag aatagaaatg gaagaacgaa aataatagcg
     1501 gctaagaact ttttcaaaat gagacgatgc gaggctctca aggcgcataa cgggcacgcc
     1561 gcgaaatatc ttgaggccca tggagagagc ggccagatgc ccttcctttt cttcgatcac
     1621 ggatcgatcg gccaggaact tctgaaagct gcctggcctg taagtgcgag accacccttg
     1681 atccactgct agctgctgga gccccgggta gacaacgcgt tccccttcct tcagaccttc
     1741 cgccatatcc cctttcagga tggtcccatc gaaatcccag gcagacagaa aaatgcatcc
     1801 gctcttgcac tcttccgttt ctgcaatctc agccttcagc tgctgcgcct ccctggcgat
     1861 ttcagcaaca tgcgaatcca cttcaggagg acgcgcacaa aaagtgtgaa ggccaatgat
     1921 gagaagcccg atccacttca ttcaacagat ccgcgtaaca ggaaggacgc agtcaatcga
     1981 agaaaaattc tccttcttgc ttgccatccc ctggcccggc tgtcagaccg tcccccatgt
     2041 attctcatca ggaatccaca ttcaagtctt caaaagatga tttccctatt tactaccaga
     2101 agtggacccc cgcgcgcacc cgccgcatcg ttgtgattca acatggcttc ggagaacact
     2161 ccggcagata tcaaaacatt cgagatgcat tcgaagggac ggacactgct ttctacggac
     2221 tcgattcgcg cgggcatgga aagacaggtg gaaagcgagg acacgttcct cagttccagc
     2281 tttacgtaga tgatcttgct gacttgattc aactcgttcg caaagagaac atgaacgatg
     2341 aagtctacct gctcgggcat tctctcggcg gtgtcatcgt tttgcagtac gctctgcagg
     2401 gaaccaacca gtccaacttg tccgcactca ttgtaagttc cccgggcctc atcatagaga
     2461 tggatgcatc caagcaagtg aagaaggtaa tggccacttt gttcgcatcc gtggcacccg
     2521 cggccactct ggaagcagga ctggatctaa agtatctgtc gcatgaccag gcggttgtgg
     2581 atgcgtacaa agcggatccg ctcgttcacg gaaagatttc tttccagatg ggaagcaacc
     2641 tcttccacct gggcaaaacc attttcgaaa aagcaaatca cctcaccatc cccgtatacg
     2701 tgtttcaggg tacagaagac aagatcgtat ctccggagag cgcaagacag ctctacaaat
     2761 ctctcactac ggaagatcgc actctcaaca tgtacgaagg tctctatcac gaaacaatga
     2821 atgagactct cgcagataga aacaaagtac tcggggattt gcgcgcctgg gttgaaaaac
     2881 actgatcctg cgggggccct gctgcgtttt caggaagtcg gccagggtca ccctctcatc
     2941 attctgcacg ggctttttgg ctcaggaaga aactggggct ccgtcgcgcg gtccctttca
     3001 gagaagtttc gcgtcttttc attagaccag agaaaccatg gagattcgcc gcagtttcct
     3061 tcgcacacgt tagacgatct tgcaaatgat cttggagaat ggatcgagtc ccatatgaaa
     3121 gatgtaccgg ttctactggg gcattctatg ggaggaatcg ccgcgatgga atacaccctc
     3181 cgacatccag accaggtgcg ggcactgatc gtagtagatg tggcgccaag accctacgag
     3241 cccagacatt ccggcgaatt ccaggcaatg gaagcagatc taagctcctg ccagaccaga
     3301 tcggatgtgg accgcgtgat ggcaccgttt gttccagact tgactgttcg ccaatttctt
     3361 caaaccaatc tgattcgcaa agatcatggc ttcggctggc gcatccccgt agaaacgcta
     3421 aagaaagctt cctttctcca gggttttgag ccaggacgat cgtggaatgg gcccaccctt
     3481 ttcatcactg gatcgcagtc agactacact tctgaatcgg atcataccgt catgcggcaa
     3541 tgctttccag aagcatacat tgaaacgatc gaaggcgcgg gccattggcc gcatttctct
     3601 catttggttc cgtttctggg tctgatcaaa gatttcctcg ggcgcgccct ctgatccgaa
     3661 ttgcacaatc gagtttgact tcaccgcgtc catggaccga tgatcataag tatggcccgc
     3721 ctgtttgcag ccatggttct gtccttgccc gccactctct gggccactcc gctactacgg
     3781 acggcagaac ctgtgattcc agaaatgcag atcctggaag acagagcaaa cctcacgcct
     3841 gagaaagcag ccgcgtctag cttcaccgtg gcaccagaac atctatcatt cgggtacaca
     3901 gactctgtca cctggctcaa gtttgacctg gaaaatcaaa ccagtgaacc tcactggctc
     3961 atttatatca gctacccact tctggatcgc gtcactctct acacgcgcga agacggttca
     4021 ctgcgagccc atgaatcggg tcagctggtt tctatgtctg cgaaagacta cgcatccaga
     4081 tacattgtgt ttcgcattac aattgaacca ggatcaagac aatcctacct ccttcgagtg
     4141 cagtctgctg attctgtggt cattcctctc caggtgatga ccttcgaatc cctggcctcc
     4201 agggaacaca atgaacaaat tgcggtgggc gttttctacg ggattgtaat acttttgatt
     4261 ctttataatc tactcatatt cttttacgca aaagaatcaa gctacttatt ttatgtgtac
     4321 tacgtaacat cctatttatt gatgcagctt tccgaaaatg gaatcctcgg ggagatgctt
     4381 ctacccgaag tgccgtggtt tgcaaaccag ctgctcgcat tcctggtcta ccatgtaata
     4441 ttcgcagcat ctgcgttctc ctatagattt cttcgcgcag aacgattcaa ggctctcagc
     4501 tacattctcc gggttttcgg tttcctcggc gtggttggaa tgtgcgcgac gttcttcgtt
     4561 ccctatatga tcgcggtgat gccgtctgta atcatagtta tgtcttttgg cccaatcgtt
     4621 gttcctctgg cgatcctgga ctggaggcgc ggaaatcgcg cagccggtca ttttgtcctg
     4681 gcctggtctg ccatactcgc cggcggattt gtatacggac tgaaagcctt cggcattctt
     4741 ccggacatgt tcatcacacg atacggcgcc atgcttggat tctgcgttca atccgttctt
     4801 ctctctctcg gtctggcaga cggaatcaaa gtactgaacc tggaactcag tcagctcaaa
     4861 acaagtctgg aacttcgcac tcgttattta gagaatatca tttcgcatgc gaaggaagcg
     4921 tcttcttcac tgatagacgc caatcaggag gaaacggcac tcgttcaagg tcttgtgagc
     4981 atggcagata gccaggcgga tctcactcat caactatcgt ctgcattcga agaactttct
     5041 gcatcttcag agtccattca cggaatcaca accagattac tcgaaaactg gcaggaaacg
     5101 cgcacgacca tttcagcgct atcttccgtg caggaaaccg tgctctcgtc cggaagagcc
     5161 gttcttgaaa acgtccttga gatttcgcag tcgtcggatg agtcgggaaa gcgtttagat
     5221 caaatgcagg atcaaatgaa agtaattcag gaaggcgggc gtgccatttc gcgctttgct
     5281 aaaaccatca gcgacataac agataagaca aacctgcttg cactcaatgc ggctattgaa
     5341 gcggcgcgcg ccggagaaca tggacgcgga ttcgccgtcg tcgcagacga aatcgccaaa
     5401 ctcgcggaag caacacagaa aagttcaaaa gaaatcttta aacaaataga aggaatacaa
     5461 aaagacatag agggaggaat ggattctgca agcgccatga gagaagcgct cggcgctgtc
     5521 ttcaccagac ttcagaaggt tcgcgcttcc atagacgatg tcaacgcatc catgtccgct
     5581 caggaggata gcatgcaggg acttctccgc cagacagcac agctggagaa cat
//
LOCUS       DAORFF010000220         5618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7865_length_5618_cov_4.015639, whole genome shotgun sequence.
ACCESSION   DAORFF010000220 DAORFF010000000
VERSION     DAORFF010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7865_length_5618_cov_4.015639"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="PKK76_09965"
     CDS             complement(<1..1617)
                     /locus_tag="PKK76_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNL68841.1"
                     /translation="MSEKLSAIIHRFYAPVIKSTTIQARLKLVLIIAVVPPLCLAAWI
                     LFNEITDLRATALEQQGLEYANAIAPMQISIMDHRQFAIDAVRGDRRTETKLRKAETE
                     VAEYSKVISKFTDRHGKEMRVFELWSKITKGWDEIMHTYSSEKDVALTFRRHTEIVQL
                     ETDMIELVGCYSGLVIDPDPATNYLIVSFSRTMPLIVNATGVERGIGRRVLITQKLPD
                     SDREDIERSMAEINLLMGRLHQDTEIAYGYDPEFKARLNALITAAEAQRARLNVTIKN
                     HVLVAQDMDPAEYGTLATNTLSAYRDLNTGISTIVSEHLHARTNSLLLKIGILSLSIV
                     FLLVLLISLVVAIVQSITNPISDAVECVQKVEQGDLTTRLDLNQDDELGILARSLNKF
                     LVSIHGVVKEMVATTGTLQGSARDLTDASQMLAAGTEQMSSQSMNIASAATQMDQNMQ
                     SVSGSAEEMSISIAEVAKQSTHAAQIAQQADQSAIGANKIIQQLGEDARKIGKVIDGI
                     VDIADQTNLLALNAAVEAARAGDAGKGFAVV"
     gene            complement(1720..2400)
                     /locus_tag="PKK76_09970"
     CDS             complement(1720..2400)
                     /locus_tag="PKK76_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNL68842.1"
                     /translation="MKSTLVRSRATSSPVASIRVFHVEDHALFRLGLQKLLEEDSGIA
                     VVGEAASAEEALERLQTTSCDIVLLDLTLPGMDGLTCLEEIKQRFPNVRIIALTMHRD
                     REFFQAAIQRQVDGYVIKEEIFERLLSAIRTVHSGSKYYSEDIRKMMVEDYATIQQSQ
                     TSLDLLSKRELDVLKLIAQGLMNKDIAKRLILSVRTVESHRARMMQKLKISNVQGLVK
                     FAMQKALI"
     gene            complement(2473..4161)
                     /locus_tag="PKK76_09975"
     CDS             complement(2473..4161)
                     /locus_tag="PKK76_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNL68843.1"
                     /translation="MNPVDCKVLVIEDNPVVTQAIRSILEERGYEPVCRSDGSSGLEA
                     AGQEDPDVILCDINLPDMDGFRILRSVQQDPRLQHIPFIFLTSRGGAEDLREGMMSGA
                     DDYLSKPFHPDELIASIQARIRRNQVIERRYRENFAMQEQRLIFSASFDETTELPLEP
                     LLKAEYETFARDENGYLVIARFPGLERIAPMVAADDVRCIIRAISERLMTGTGSAVFR
                     IGRDELAFFVLNADEAGASVHAEQCLRLFHSCLECNERLLHLPGLFGMSQLKHDVPFE
                     SALRNARVAAKYAEASGGTGFRIFDESMETRLGDQLFLESELRQALARNEFHLVFQPR
                     VLLPGRVVGAEALVRWTHPRLGNVPPSVFIPVAEESGTIDQIGNWVLSESAKKLSEWK
                     MQGLPEDFTLAVNVSGRQLESADFLPLLQSLGGHTNSLELEITETFLIKDIHRSVALF
                     KQVRELGLQLAVDDFGCGYSSLRYLKMLPLNKLKIDRSFVRDLPGDESDANIVRAICQ
                     LGKSLGLILVGEGVEKVEQADFLASHGCEEVQGFLYSPPIVEKEFLAFARRSWS"
     gene            complement(4241..5404)
                     /locus_tag="PKK76_09980"
     CDS             complement(4241..5404)
                     /locus_tag="PKK76_09980"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNL68844.1"
                     /translation="MPISEIPGKIPVKLWTRLEDVESGALVQLKNIASLPWVFRHVAA
                     MPDVHLGIGATIGSVIATREAISPAAVGVDIGCGMGAVQTSLKASDLPDHLGAIRSRI
                     EAMIPVGHKEHSQARVRKSEVKFFSEFGKLHPLVQKLEKKAIHQCGTLGGGNHFIELC
                     LDTEQRVWLMLHSGSRNIGKTLAELHISIAKKLIHNQKLPDRDLAVFLSGTPEMEAYR
                     RDLYWAQRYAFLNRETMFEIYQDVMREFFPSVKFQRPIMCHHNYVAEEVHFGEKVFVT
                     RKGAIRAGRGEMGIIPGSMGTRSFIVRGLGHPDAFESASHGAGRRMSRTQAKRTFTEK
                     DLREQTSGVECRKDHGVLDEIPGAYKDIAQVMADQSDLVGIVHELKQVLCVKG"
     gene            complement(5404..>5618)
                     /locus_tag="PKK76_09985"
     CDS             complement(5404..>5618)
                     /locus_tag="PKK76_09985"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HNL68845.1"
                     /translation="EMSAKEQMEELRRAMLQAAANMEFEKAALLRDAMLSVEKGEKVD
                     FMKLAQKKSGKKQGQTTQMRGRKGRR"
BASE COUNT         1343 a         1534 c         1315 g         1426 t
ORIGIN      
        1 aacaacagcg aatccttttc cagcatctcc cgcacgggct gcttcaaccg cagcattcaa
       61 agccagcagg tttgtctggt ccgcgatgtc tacgatgcca tctatcactt tgccaatctt
      121 tcgcgcatct tcaccgagct gctggataat cttgtttgcg ccgattgcgg attgatccgc
      181 ctgttgtgcg atctgcgctg cgtgcgtgga ctgcttggca acttccgcga tggaaataga
      241 catctcttcc gccgaacctg aaacagattg catattctga tccatctggg tagcggcaga
      301 cgcgatgttc atagactgcg aagacatctg ttccgtcccc gccgcaagca tctgacttgc
      361 gtctgtcaaa tcgcgcgcgg aaccctggag ggttccagta gtggcgacca tttctttcac
      421 aacaccgtga attgaaacca ggaacttatt gaggcttctg gccaatatcc cgagctcgtc
      481 atcctggttc aaatccaggc gcgtggtcag gtctccttgt tccactttct gaacgcactc
      541 cactgcatcg gaaatcggat tggtgatact ctgaacaatt gcaaccacga gcgaaatcag
      601 aagcacaaga aggaatacta tagaaagact tagtatccca atcttcagaa ggagagagtt
      661 ggttctcgcg tgcaaatgct cagaaacaat cgtcgaaatc cctgtattca ggtcccggta
      721 agcagatagc gtattggttg caagcgtccc atattccgca ggatccatat cctgagcaac
      781 cagcacgtga ttcttgatag tcacgttcaa acgtgcgcgc tgggcttctg cggctgtgat
      841 cagggcattg agccgggcct tgaactccgg atcgtaacca tacgcgatct ccgtatcctg
      901 gtgcaagcga cccatcaaca ggtttatttc agccatcgat ctttctatgt cttcgcggtc
      961 tgaatccggc aacttctgcg tgatcagaac gcggcgtcca attcctcgtt ccactccagt
     1021 tgcgttcaca atgagaggca tagtgcgtga gaaagaaacg atcaaatagt tcgtggcagg
     1081 atccggatcg atcaccaggc ctgaataaca cccgaccagc tcgatcatgt ctgtctcaag
     1141 ttggacgatt tcagtgtgcc tgcgaaaggt gagggcaacg tctttctcag aactgtacgt
     1201 gtgcatgatc tcgtcccagc ccttcgtgat tttagaccat agttcgaaga cgcgcatttc
     1261 cttgccgtga cggtccgtaa actttgaaat gactttgcta tattcagcaa cttccgtttc
     1321 agccttccgt aatttcgttt ctgtcctacg atcgcctcgc actgcatcga tggcgaattg
     1381 acgatgatcc atgatggaga tttgcatggg agcaatcgca ttggcgtatt ctagtccttg
     1441 ctgttccagc gcggtcgcgc gaaggtcggt gatttcattg aacagaatcc aggccgcgag
     1501 acacagtgga ggaacaactg cgatgatcag caccagtttg agtcgcgcct gaatcgtcgt
     1561 gcttttgatg accggcgcat aaaaacggtg gatgatggcg gacaacttct cagacacctg
     1621 cgaacctcat gtttctgatt tacagtaaca tgaatgtcag gatggttcaa ttggtagtgg
     1681 tgcttgtttt ttcagtacga gtacggaacc ttgcaatggt taaatgagcg ctttctgcat
     1741 ggcgaatttt acaaggccct gcacattcga aattttcagc ttttgcatca ttcgcgcgcg
     1801 atgagactcg actgtacgaa cactgagaat caatctcttc gcgatgtctt tgttcatcaa
     1861 tccttgtgcg atgagcttga gcacatccag ttcacgtttg gagagtagat ccagcgaggt
     1921 ttgtgattgc tggatcgtgg catagtcctc caccatcatc ttgcgaatat cttctgaata
     1981 gtattttgaa ccggaatgaa ccgtgcgaat ggcggaaagt agcctttcaa agatctcttc
     2041 tttgatcacg tatccgtcca cctgccgttg gatcgccgcc tgaaaaaact cgcgatcccg
     2101 atgcatagtc agagcgatga tacgaacatt tggaaatcgt tgcttgattt cctcgagaca
     2161 ggtcaatcca tccattccag gcaacgtgag atccagtagc acaatgtcac acgacgttgt
     2221 ctggagcctc tcaagtgctt cctctgcact cgctgcttcg ccaaccaccg cgatgccaga
     2281 atcttcttcg agcagtttct ggaggccaag gcgaaaaagc gcatgatcct ccacatgaaa
     2341 aacacggatg gaggcgaccg gcgacgaagt cgcgcgagat ctaacgagag tagatttcat
     2401 acgaccagac caatgtacgc gaagagagat tgggcggcaa gacagttcag attatttatg
     2461 ttcgctttcg ccttacgacc atgaacgacg tgcgaaagcc agaaattcct tttccacaat
     2521 tggagggctg tacagaaacc cttgcacctc ttcgcatcca tgcgaagcca aaaaatcggc
     2581 ctgctccacc ttttctacgc cttcaccaac gagaatgagt cccaaactct ttccgagctg
     2641 gcatatcgca cgcacgatgt tcgcgtccga ctcatctcct ggcaaatcgc gcacgaaaga
     2701 gcgatcgatc ttcaacttgt tgagcggaag catcttcaaa tagcgaagcg aagagtagcc
     2761 acatccaaaa tcgtctacag ccagctgcag ccccagttcg cgtacttgct tgaatagggc
     2821 aacggaccta tggatgtcct tgatcaagaa cgtttctgtg atttccagtt cgagagaatt
     2881 cgtgtggccg ccgagagatt gcagaagagg cagaaaatcg gcactttcaa gctggcggcc
     2941 gctgacgttc actgccagag taaaatcctc cggcaaacct tgcattttcc attcggataa
     3001 cttcttcgca ctctccgaaa gcacccagtt tccgatttga tcaatggtcc cggattcttc
     3061 cgcgactgga ataaaaaccg acgggggaac attgcccagc ctgggatggg tccagcgcac
     3121 gagggcttct gcgcctacga cgcgacccgg aagcaaaaca cgaggctgaa aaacgagatg
     3181 aaattcattt cgcgcgagag cctgacgcaa ttcactctcc agaaatagtt ggtcccccag
     3241 gcgagtctcc atggattcat caaaaatgcg aaagcctgtt ccacccgacg cttccgcata
     3301 ctttgccgcg acgcgtgcat ttctcagcgc gctttcgaat ggcacatcgt gcttgagttg
     3361 ggacataccg aacagcccgg gcagatgaag aagtcgctcg ttgcactcaa ggcaagagtg
     3421 aaagaggcga agacattgtt ctgcgtgaac tgaggcccca gcctcgtccg cattcaggac
     3481 aaagaatgca agctcatcgc gccctatgcg aaacactgcg gaacccgtcc cagtcatcaa
     3541 ccgttccgaa atagctcgaa tgatgcagcg aacatcatct gcagctacca tgggcgcaat
     3601 tctttcgaga ccaggaaacc tggcgatcac caggtatcca ttctcgtctc ttgcgaaagt
     3661 ttcgtattcc gcctttagaa gtggctccag tggaagctcc gtagtttcgt cgaagcttgc
     3721 ggaaaaaata agcctctgtt cttgcattgc gaaattctcg cgatatcttc tctcgatgac
     3781 ctggtttctt ctgatccgag cctgaataga ggcaatgagt tcatctggat gaaacggctt
     3841 gctcaagtaa tcgtccgcac cggacatcat tccttcgcgc aaatcctccg cgcctccacg
     3901 gctcgtgaga aaaataaacg gaatatgctg aagcctggga tcctgctgca cggaacggag
     3961 gattctaaaa ccgtccatgt ctggcagatt gatgtcacac agaatcacat ctggatcctc
     4021 ttgacccgca gcctcaagtc cactcgaccc gtctgatctg cagacgggtt cgtaacctct
     4081 ttcttcaagg atggatcgaa tcgcctgagt aaccacagga ttgtcttcga ttacaagcac
     4141 tttgcaatct accggattca cgcccacatc ctacaatgcg ggaagattag tttcaatagg
     4201 tagtgcgact atttttttga gtacttctac gtacgcgact ttagcccttc acgcacaaaa
     4261 cctgtttcag ttcatgaacg attcccacca gatccgattg atccgccatc acctgtgcga
     4321 tatctttgta cgcgccagga atttcatcca gtactccgtg atcctttctg cattccactc
     4381 cgctggtttg ttcgcgcaaa tctttttccg tgaaagtgcg cttagcttga gttctgctca
     4441 tgcgacgacc tgcaccgtgc gaagcgcttt cgaaggcatc tggatggccc agccctcgaa
     4501 ctatgaatga tcgtgttccc atggaccccg gaatgattcc catttctcca cgtcctgcac
     4561 ggatggcgcc ctttcgcgta acaaaaacct tctcaccaaa atggacttcc tcggctacat
     4621 agttgtgatg acacatgatt ggtctttgaa actttacgct cggaaagaat tcacgcatca
     4681 catcctgata aatttcaaac atggtttccc tgttcaagaa ggcatatctc tgcgcccagt
     4741 aaaggtcacg tctgtacgcc tccatttccg gtgttcccga gaggaaaacc gccagatctc
     4801 gatccggcag cttttgattg tgaatcagct tctttgcaat ggaaatgtgt agttccgcta
     4861 gcgtcttacc aatgttacgc gatccagaat gaagcatcaa ccagactctc tgctctgtat
     4921 ccagacaaag ctctataaaa tggtttccgc cacccagtgt gccacattga tgaattgctt
     4981 tcttttccag tttctgcacc aggggatgaa gctttccaaa ttcggaaaaa aacttaactt
     5041 ctgattttcg cactcgagcc tgagaatgct ccttgtgtcc taccgggatc attgcttcta
     5101 tgcgcgatcg gatggctccc aggtggtccg gaagatctga cgctttcagg ctggtctgca
     5161 ctgctcccat tccacaaccg atgtccactc cgactgccgc gggcgaaata gcttcgcgtg
     5221 tcgcaatcac agatccgatc gttgcgccga ttcccagatg aacatctggc atcgcggcca
     5281 catggcgaaa tacccacgga agagaggcga tattcttcag ctgcacaagc gcgccgcttt
     5341 ccacatcctc aaggcgcgtc cacagtttga caggaatctt acccgggatt tccgaaatcg
     5401 gcactaacgc ctgccctttc tgccgcgcat ctgtgttgtc tgtccttgct tctttccgct
     5461 ctttttctga gcgagtttca taaaatcgac tttctcccct ttttccacgg acagcatcgc
     5521 atcgcgaaga agggctgcct tttcaaactc catattggcc gctgcctgta gcatggcccg
     5581 gcggagttct tccatctgct cttttgcaga catttcat
//
LOCUS       DAORFF010000221         5610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7883_length_5610_cov_3.530693, whole genome shotgun sequence.
ACCESSION   DAORFF010000221 DAORFF010000000
VERSION     DAORFF010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7883_length_5610_cov_3.530693"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1377
                     /locus_tag="PKK76_09990"
     CDS             73..1377
                     /locus_tag="PKK76_09990"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNL68846.1"
                     /translation="MCDTAVALPNATREGITILAKNSDREPNEAQAIVRFDAAQHSEK
                     TLRVSYIEIPQVKETFEVILSKPFWMWGAEMGVNSKGVAIGNEAVFARVPFAKKNDGL
                     LGMDLLRLALERSDTAQNAMLCITQLLEQYGQDAMAGYYDKNFYYHNSFIIADPTEAY
                     VLETIEKHWAFVKVKDVRSISNGLTIETEFDGSSKGLIEFAKSKGLHKGGEFNFRTTF
                     SDKFFTYFSMCASRRADTEGNLRQSEGKTDARSAMSHLRSHGLHEKQGFRPDKSSMAS
                     VCLHAKGPVTPSQTTGSMVAELRKDGRHTVWLTGSSSPCLSLFKPFYFGTDTSPQSLC
                     GIPGSSADDSLFWKHERLHRRALKAYGPSHEMIRQAYDSLEEEFLRSEHDRFGQGSFS
                     SECVQRQLSAMQALDLPRPGSAVFHPLYRMQWNKWNSLCGIV"
     gene            1472..2242
                     /locus_tag="PKK76_09995"
     CDS             1472..2242
                     /locus_tag="PKK76_09995"
                     /inference="COORDINATES: protein motif:HMM:NF025595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 family protein"
                     /protein_id="HNL68847.1"
                     /translation="MSTMEHSEHISHAGHSHDGHGGHGASFGMRVGITMALLGVLLAF
                     SAAKVGGERAELVKSLVEQQNAHAKYQGNDVKHRVAAIALFQVHAEVAGASKGKSLNK
                     EDVGFLAQNVERYLQESAAAKTWVESYDPLIEVHVEAQEYYEFGQLSAEVAIVVASVA
                     LLLRRKEAWFVAVGLGILSIGIVIATFVHSSHIAHASEQKIEENFKAYREMRNAHKTT
                     QVEEKVVDEMLAWSGRPHLAKLESERHPESPGAKPDHK"
     gene            complement(2285..3670)
                     /locus_tag="PKK76_10000"
     CDS             complement(2285..3670)
                     /locus_tag="PKK76_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68848.1"
                     /translation="MRFIRLIVLSVVAISAISCRLDSQVLAEFNGGDVRRSELRAAFR
                     LMEGQPSLEMQDRVVTDLALLKITALEAVKQGLDQTEDFKKREPLFHKLAFLGSLEQE
                     LMAEQKKQKFKMMELQFAFLKEGEKDKKRKPEAEDIAKKLNEASDSDREKIIAQKTEM
                     KRYAVTGGYADPICVSCVPNPFAFLTDAAAKAEGKFVVVDDPNGFWIIRKAQETEVSG
                     DSLSETYEAYFKKAARLSRTAIAAMPEGAEKKEMEAQTMKEAQIETVSKQIAEQQVKR
                     ESQGLLQKRIEQRKKSLGLEALPGLRSYFDEKTDVAKDLSAPVIKMGGKDITLGELLQ
                     GLPEDKTVDRRQLLQQIVLPYEILKEDPLAAAALKSPQHDFLMAWNRNNQLAVLFLGK
                     QNLPEITEAQVLERFNAGFAGQNFGQLHDRIKADLTQEMRQGKLKQIQDGLGKQYNLK
                     INKDKLKGGEL"
     gene            complement(3718..4113)
                     /locus_tag="PKK76_10005"
     CDS             complement(3718..4113)
                     /locus_tag="PKK76_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68849.1"
                     /translation="MMKNAAPFEGVKLRAVHLLRHLERVERDLEELKRLEQGLKDDRA
                     YTARLRDSLVDEQLRLEQSRARLLQQVVKAPPEKVVDAVQNQLDAIAAPQRRAPELIL
                     PEGKRKTEEKKPKRAVEEKKKPSFNFKYD"
     gene            4154..4996
                     /locus_tag="PKK76_10010"
     CDS             4154..4996
                     /locus_tag="PKK76_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68850.1"
                     /translation="MGIVLDLKDPGWKMTPMQVIRILPRNEAFDFNFKRKAWHMLGLL
                     VPAFFYFDPLVILGTPYIHITRMVGLGLNVFMLLALIVIDVLRFRVESLNQLFIRVAG
                     PLLKEQEHGRMNATIPYLTATLLLFSSTQEIIAGCATTFLMLGDPVAAYIGGKFGRVR
                     FWNSKSLEGMLAFFLAGTTAAGLFLLLHSWLILQGFPNAVREAEYSFFGNRPLALLAV
                     LVVGSAAAAVTEFFSSNRLAGLLDDNLEVPIMAAIAMAVTANLCGFSGESIFFDLKQI
                     FARG"
     gene            4993..>5610
                     /locus_tag="PKK76_10015"
     CDS             4993..>5610
                     /locus_tag="PKK76_10015"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNL68851.1"
                     /translation="MKRILDRYVFFEFLKSFVFSLLGLILMLLIARALVAFKVDTRQP
                     KIHMYLYLLYSIPDMVAFVLPMAMMFAVCFVVAQFTVSRELVAMLSAGVAFYRIVMPI
                     WIFAAILVPVLFVFQNFVATPANRMSSEEEAAFKKDSATIKDIVWQRNLRGREGFYFI
                     YYLDRKEQTIKGGFNYIELKHDKPVRMMQANSGKFDPATGEWTFSR"
BASE COUNT         1258 a         1373 c         1443 g         1536 t
ORIGIN      
        1 agcctttcgg aagcggtttc caatacgatc caaaaaactt gtctgatctg accgactctt
       61 cataacttgt ccatgtgcga cacagccgta gccctgccca atgccaccag agaaggaatc
      121 accattcttg ccaagaattc tgacagagaa cccaatgagg cccaggcaat cgtccgattt
      181 gatgcagcac agcattcgga gaaaacttta cgtgtatcat atattgagat tcctcaagta
      241 aaggaaactt ttgaagtcat cctctcaaaa cccttctgga tgtggggcgc ggaaatggga
      301 gtcaactcga aaggagtggc gatcgggaat gaggcagtgt ttgctcgcgt tccttttgcc
      361 aaaaagaatg acggccttct cgggatggac ctgcttcgcc ttgcgcttga aagaagtgat
      421 acagcacaaa atgcaatgct ctgcatcacc caactgctgg agcaatacgg ccaggacgcc
      481 atggccggct actatgataa gaatttctat tatcacaatt cgttcatcat cgccgatcca
      541 acagaagcat acgttctaga aacaattgag aaacactggg cgtttgtgaa agtgaaagat
      601 gttcgttcca tttctaacgg actcaccatt gaaacagaat ttgacggaag ttcgaaaggc
      661 ctgattgaat ttgcgaaaag caaaggctta cacaagggcg gggagttcaa tttcagaact
      721 actttttcgg ataagttctt cacatatttc tccatgtgcg cgtccagaag agcggacaca
      781 gaaggaaatc tccggcagag cgagggaaag acagacgcca gatccgccat gagccatctg
      841 cgatctcatg gcctgcacga gaagcagggc tttcgtcctg acaaatcgag catggcttcc
      901 gtatgccttc acgcgaaggg cccggtaaca ccaagccaga ccacgggttc catggtggcg
      961 gagctgagaa aggacggccg acacactgtg tggctcaccg gatcgtcttc gccatgtttg
     1021 tcgctgttca agccgttcta tttcgggaca gacacgtctc cgcagtcctt gtgtggcatc
     1081 ccaggatctt ctgcagatga ttcgttgttc tggaaacacg agcggcttca cagacgggca
     1141 ctgaaagcat acgggccctc tcatgaaatg attcgccagg cttatgattc tctcgaagaa
     1201 gaatttttac gctcagagca cgatcgtttt gggcagggga gtttttcttc cgaatgtgta
     1261 cagcggcagc tttctgccat gcaggctctc gaccttcctc gtcctggctc cgcggtcttt
     1321 catccgctct atcgcatgca atggaacaaa tggaattctc tttgcgggat tgtctgagca
     1381 ggtcaggcgt tgtctcaaaa aaagattcga ccggatcgtc tctgactcat tcattgcacg
     1441 cgacggagcc actcgcttcc tggagatcta gatgagtaca atggaacaca gcgaacacat
     1501 atcacacgcc ggtcattcac acgacggtca cggtggacat ggagcctctt ttggaatgcg
     1561 cgttggcatt acgatggcgc tgcttggagt tcttcttgcg ttttccgcgg cgaaagtggg
     1621 aggtgagcgc gcagaacttg tgaagtctct cgtcgaacag cagaatgctc acgcgaagta
     1681 tcagggaaat gatgtgaagc accgcgtggc agccattgct ctttttcagg tgcacgcgga
     1741 agtggcggga gcaagcaaag gaaagtctct caacaaagag gacgttggat ttcttgccca
     1801 gaacgtggag agatatttgc aggagtctgc tgcagcaaag acctgggttg aatcctacga
     1861 tcccctcatt gaagtgcacg tggaggcgca ggaatactat gagttcggcc agctgtctgc
     1921 agaagtggcg atcgtggttg catctgtcgc gctcttgctc agacgcaaag aagcatggtt
     1981 tgtggcagta ggtctgggta ttctcagcat cggtattgta atcgctacgt ttgttcattc
     2041 ctctcacatt gcgcatgctt ccgagcagaa gatagaagag aacttcaaag cctaccgtga
     2101 gatgcgaaac gcacacaaga caacgcaggt agaagagaag gtagtggacg aaatgctcgc
     2161 gtggtctgga agaccgcacc ttgcaaagct cgaatcagag aggcatccag aatcgccagg
     2221 cgcgaaaccg gatcacaaat agagtggtac ggctcctggt gtgatccacc aggagcgtcg
     2281 cggattagag ttcgccgccc ttaagtttgt ctttgttgat cttgagattg tactgcttgc
     2341 ccaggccgtc ctggatctgt ttgagttttc cctgacgcat ttcctgagtt agatctgctt
     2401 tgatgcgatc gtgcagttgt ccgaaattct gtcctgcaaa tccagcattg aaccgttcta
     2461 gaacctgagc ttcggtaatt tctggcagat tctgtttccc gaggaagagg acagcgagct
     2521 gattgtttct gttccatgcc atcaagaaat catgctgagg cgatttgaga gcagcggcgg
     2581 caagtggatc ttcttttagg atttcataag gaagaacgat ctgctgcagt agttgtctgc
     2641 ggtctacggt cttgtcttcc ggcagaccct gaagaagttc accgagcgta atgtctttgc
     2701 ctcccatttt gatgacgggt gcgctcagat ctttggcaac atctgttttt tcatcaaaat
     2761 aggaacggag gccaggaaga gcttcgagtc cgagcgattt ctttctctgc tcaattctct
     2821 tttgcagcaa gccctgggat tccctcttta cctgttgttc tgcaatttgt ttggacacag
     2881 tttcgatctg cgcttccttc atggtctggg cttccatttc ttttttctca gccccttccg
     2941 gcatggccgc aatcgcagtg cgcgaaagtc tggcggcctt tttgaagtat gcttcgtatg
     3001 tttcagaaag agaatcgcct gacacttctg tttcctgagc tttgcggatg atccagaaac
     3061 cgtttggatc gtctacgact acgaacttgc cctctgcttt tgcagcggcg tctgttagaa
     3121 atgcaaatgg attgggaacg cacgatacac agatcgggtc agcatagccg cctgtgaccg
     3181 cataacgttt catttcagtt ttctgtgcga tgatcttttc gcggtcggaa tcggatgcct
     3241 cgttcaattt tttggcgatg tcttccgctt ccggtttgcg tttcttgtct ttttcgcctt
     3301 cctttagaaa ggcaaactga agttccatca tcttgaactt ctgtttcttc tgttctgcca
     3361 tgagttcttg ttccagcgaa ccgaggaagg cgagtttgtg aaacagaggt tcgcgttttt
     3421 tgaaatcttc tgtctgatcg agaccctgct tgactgcttc gagagctgtg attttgagaa
     3481 gagccagatc tgtcaccact ctgtcctgca tttcgaggct gggctggcct tccatgaggc
     3541 gaaaggcggc acgcagttcg gatcggcgaa catctccgcc gttgaactct gcgagaacct
     3601 gggaatccag gcgacaggat atggctgaaa tcgccactac agacaaaaca atgagtcgaa
     3661 tgaaacgcat tgttcgattt tgccgggaca ggtgtgcggg caagtacaat cggcgattca
     3721 atcgtacttg aagttgaagg atggcttctt cttttcttcc accgcgcgct ttggtttttt
     3781 ctcttccgtc ttgcgtttgc cttccggtag aatcaattca ggggcgcgcc gctgtggcgc
     3841 cgcaatggca tcgagctgat tttgcaccgc atcgacgact ttctccggcg gtgccttgac
     3901 tacttgctgg aggaggcgag cgcgggattg ctcgaggcga agttgctcgt cgactagaga
     3961 gtcgcgcagc cgcgctgtat atgcgcgatc atccttgagt ccctgttcca atctcttgag
     4021 ttcttccagg tctctctcca cccgttcgag gtgacgcaag agatgcaccg cgcgaagctt
     4081 cacgccttca aaaggagctg cgtttttcat cattgtcaag ttcaattttc ggcaggcgcc
     4141 agggcagtct gaaatgggaa ttgtactcga tttaaaggac cccgggtgga agatgacccc
     4201 catgcaggtg attcgaatct tgccgcgaaa tgaggctttt gacttcaatt tcaaacgcaa
     4261 ggcctggcat atgctcgggc tcctcgtgcc cgcgttcttc tactttgatc cacttgtgat
     4321 cctcggaacg ccttacattc acatcactcg catggttgga ttgggtctga acgtctttat
     4381 gcttctggcg ttgatcgtca tagatgttct tcgctttcgc gttgaatctc tcaatcagct
     4441 gttcattcgc gtggcaggac ctctcctcaa agaacaagaa catggccgaa tgaacgcaac
     4501 cattccatac ttgactgcga ctctactttt gttttcgagc acacaggaaa tcatagctgg
     4561 ttgtgccacc acgtttctca tgctgggaga tccagtggcc gcttatattg gtggaaaatt
     4621 tggacgagtt cgtttttgga attctaaatc gctagaagga atgctcgctt tctttctggc
     4681 tggaaccacg gccgctggtt tgtttcttct cctgcattcc tggttgattc ttcagggatt
     4741 cccgaatgct gtacgcgaag cagagtattc tttttttgga aatcgccctt tagcacttct
     4801 ggcagtgctc gtagtgggat ccgcggcggc cgctgtgact gaattttttt cttcaaatcg
     4861 attggccgga cttctagacg acaacctgga agtgcccatc atggctgcca ttgctatggc
     4921 ggtcactgcg aacctgtgcg gcttttccgg tgaatcaatt ttctttgatc tcaaacagat
     4981 cttcgcgcgc ggatgaaacg gatactggat cgttacgtat tttttgaatt cctgaagtct
     5041 tttgtcttct cgcttctcgg attgattttg atgcttctca tcgcgcgggc actcgttgcc
     5101 ttcaaggtgg acacacgtca gccgaagatt cacatgtatc tgtatctatt gtattctatt
     5161 cctgacatgg ttgcatttgt tctccccatg gccatgatgt ttgcggtttg ctttgtcgtg
     5221 gctcagttca ctgtctcgcg cgaactcgtt gcgatgcttt ccgcgggtgt tgccttttac
     5281 agaatcgtaa tgcctatctg gatttttgcg gctatccttg tgccggttct gtttgtgttc
     5341 cagaatttcg tggccacgcc ggcgaatcga atgtcatcag aggaagaagc tgcatttaag
     5401 aaagacagcg ctacgatcaa agacattgta tggcagagga atctgcgcgg ccgcgaaggc
     5461 ttttatttca tctactatct ggatcgcaaa gaacagacca tcaaaggcgg gttcaactac
     5521 atagaactca agcacgacaa acctgttaga atgatgcagg cgaattcagg caaatttgat
     5581 cccgcgactg gcgaatggac tttttcgcgt
//
LOCUS       DAORFF010000222         5597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7916_length_5597_cov_4.177914, whole genome shotgun sequence.
ACCESSION   DAORFF010000222 DAORFF010000000
VERSION     DAORFF010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7916_length_5597_cov_4.177914"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1744)
                     /gene="asd"
                     /locus_tag="PKK76_10020"
     CDS             complement(<1..1744)
                     /gene="asd"
                     /locus_tag="PKK76_10020"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNL68852.1"
                     /translation="MNEIISYVSIPVISLILFAGLFLTIRFQGVQLRELLLSIKVLSG
                     MMDQPSGKEQHTMFQAFLLSLAGVIGGSVVLAGTHILDSRTWSWFLIFAFIYAPIQFS
                     VGATLSRNTRSIAVLAALALIGLQGVSQTSYTASIEMHLAWNPLAVGLIVTALSAGIV
                     FAPVRIGASIFAWSGLLFVIYVIRNAFVGGPGSLPGLYFSTNPVHISSALLAALTLCE
                     IARAPFLFVSVRSSGAKPALVSMLSVLLSPFVFAIVFYVLDAFIPSDVRTMSLAAIVG
                     LVLWTASWMWTIRSFVPGSKGTLLSLAFALLPLAMQHTSYSMMVDILVFGSAAGGLAL
                     ACFTVEAFLRSSEASQSLAELRESHRWEISKDIYLIFLTILPQNLVSRIFGWIAATPL
                     PGFLREPIILAYSRAFGVTVEEAAKEIREYSSLNNFFIRYLKPGARPIEGDEKTIVSP
                     VDGTVLRFGDITEGLLIQAKAMQYSLRDLLEFPDYLQDFEGGKFLVIYLSPRDYHRIH
                     FPVGGTVPAFAYSPGDLFTVNPVAVERLDALFAKNERLATYIHNPRGTVALIKVGATN
                     VGRIVLAYDSFKTNR"
     gene            1731..2627
                     /locus_tag="PKK76_10025"
     CDS             1731..2627
                     /locus_tag="PKK76_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68853.1"
                     /translation="MISFMMQSDAVLCRVGGNPVKAFCLSALVAWLIMSAPGLEAIPV
                     EIGISGVGSQRNPGTFDQGLDNYRTSLNPLVFSLTKHSNFRSIPASEGYIRLGDLISE
                     ESYIGLLYGRLYTPPISVRQFRSDAVYFQSNWNFFVHYFGFTYHYRFRPISSSRMSYE
                     VGGAYTFVAEPTWRTDGYYLSPYQFSRLQTSHRAGYGSMLRLEGAVVRGLGDHFFVRA
                     GIAQSYWYMGRYQGNVNSTGGYWVTLRNGGFLALAPSQFISTFVVSPDPILGPTQVST
                     IRDRADINFSLTQFFVSAGLRF"
     gene            2671..2910
                     /locus_tag="PKK76_10030"
     CDS             2671..2910
                     /locus_tag="PKK76_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68854.1"
                     /translation="MSAEKVITMPVREIADVKVILATWYKFLQDQADKLNAEEFSRSL
                     KTPIILNLETDELELLFTGSGELLQGFKDFILTTT"
     gene            complement(2918..3592)
                     /locus_tag="PKK76_10035"
     CDS             complement(2918..3592)
                     /locus_tag="PKK76_10035"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNL68855.1"
                     /translation="MCGRFALFEEKEVLEGFFGFQIDLDFGPHYDIRPTDQVLSFAHH
                     GASLMRWALLPAWEKSNQVRYATFNARLEEAATKPAFRSSVIANRRCVIPASGFYEWK
                     DVAKKKKDRYYFRRLDGKPLAMAGLWDTWKGGGVEIVSCTILTTEANELIRPIHAKHR
                     MPVILDANEISEWLDPNTPSDSLFRKHATGFAAGRMETYKVDSRLREDDKRLVEKYEE
                     PELFKL"
     gene            3688..4173
                     /locus_tag="PKK76_10040"
     CDS             3688..4173
                     /locus_tag="PKK76_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68856.1"
                     /translation="MRSIVFAVLVAIIAPLMAQTKANDIPVEKLPADVKQVLEQYVNI
                     LRTSKSLDECATRFTSIAGGSLVTDEKGTLRDTVKPFSLKKDFNDIKQYADPIVITRV
                     NATPSNGQGYGSSAIKGMVYKIWIAKKDASIGMPAPVSILVPEGHPTITTPKVVGIGS
                     Y"
     gene            4223..5431
                     /locus_tag="PKK76_10045"
     CDS             4223..5431
                     /locus_tag="PKK76_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68857.1"
                     /translation="MYQLFIQIIKYGFSTVLFGVGLYVLIASYREHKSFRRLFKGFAI
                     TAYFLFAAGMTLLIFTGETAAQLSMFFIKAISTAFGLLYGIYATLNGFMETDPATGKK
                     TVTRLGVLGIFILSFNTILLVSMDYLQETAAMEEKLQEKQLQLDQERERKIEYERLLG
                     SVSFVDRKTGDLKIDFEKFSESLYRSLVTVETDLRNTRIELATRETELKDATARWNAA
                     DAGAKVLQETLSKTEKALATRDAELKESQTKLAQSDASLKAAQENLAKTEKSLAGREA
                     ELKNTSDQLVESQKEAKKAEEELSRTSRDLASKDAEAREQTRRATAAESSLARAQDEL
                     KKSAAAFTTRDAEARQYQKENLTLQTNLARETEERSKLDKLLAARDAELRACKTAPAT
                     PTPPQPVPPG"
BASE COUNT         1562 a         1434 c         1378 g         1223 t
ORIGIN      
        1 agcgatttgt cttaaatgaa tcgtatgcaa gtacaatcct tcctacattc gttgctccca
       61 ctttgatcaa agccacagtg ccacgcggat tgtgaatata tgtggccagt ctttcgttct
      121 tagcaaacaa cgcgtccaat ctttccacgg caacggggtt cacagtaaaa aggtctcccg
      181 gagaataggc gaaagcggga acagtgccac ccactggaaa atgaattcta tgataatcgc
      241 ggggactcag ataaatcacc agaaactttc cgccttcaaa gtcctggaga tagtccggga
      301 actccagcaa atcacgcagc gaatactgca tggcctttgc ttgaatgaga agcccttctg
      361 tgatgtctcc gaacctgaga acggtcccat ccacaggaga cacaatggtc ttttcgtctc
      421 cttcgatggg gcgagcccct ggtttcaaat aacggatgaa gaagttgttc aatgaggagt
      481 actcacgaat ttcttttgcg gcttcctcta cggttacacc aaatgcacgc gaatatgcca
      541 ggatgattgg ttcgcgcagg aatccaggca gaggagtggc tgcaatccag ccaaatattc
      601 tggagacaag gttctgaggc agaatcgtta agaatattag ataaatatct ttactgatct
      661 cccaacgatg agattcgcga agctcagcga gtgattgaga tgcttcgctg ctacgaagaa
      721 acgcctctac tgtaaagcat gcgagagcca gaccgcccgc cgcggatcca aacacaagta
      781 tgtccaccat catggagtag gaagtgtgct gcatggcaag tggcagaagc gcgaaggcta
      841 aagaaagaag agtgcccttt gagcctggaa caaaagaacg aatggtccac atccaggaag
      901 cggtccagag gaccagaccc acaatggccg caagactcat agtgcggaca tcagaaggaa
      961 tgaacgcatc taacacgtag aatacgatcg caaacacaaa aggagaaagc aaaaccgaaa
     1021 gcatggacac aagcgccggc tttgctccgg acgagcgaac agatacaaaa aggaaggggg
     1081 ctcgggcaat ttcgcataac gtcaacgccg cgagcagagc tgaagaaata tgaaccggat
     1141 tggttgaaaa atacagtccc gggagagaac ctgggccacc aacaaaggcg tttcgaatca
     1201 cgtatatgac aaacaaaagg ccggaccagg cgaaaatgga agccccgatt ctcacgggag
     1261 caaaaacaat gccagccgaa agcgcggtca caatcaaacc gactgcaagc ggattccatg
     1321 cgagatgcat ttcaatcgaa gccgtataag aagtctgaga gacaccctga agaccgatga
     1381 gcgctagagc ggctaacact gcaatcgaac gagtgttgcg cgacagggtt gcgcccacgc
     1441 taaattgaat gggtgcgtaa atgaaggcga agattaagaa ccaggaccac gtgcgagagt
     1501 ccagaatgtg agttccggca agtacaacgc ttcccccaat tacgcctgcg agagaaagca
     1561 agaacgcctg gaacattgta tgttgctctt tgcccgatgg ttgatccatc ataccgctga
     1621 gcaccttgat ggacagaagc aattcgcgta gctggacgcc ctggaatcga atggtcagga
     1681 aaagccccgc aaataggatc agcgaaatga cggggatcga tacgtaagaa atgatttcat
     1741 tcatgatgca atcggatgca gttctatgcc gcgtgggagg caacccggtc aaggcatttt
     1801 gcctgagcgc actggtcgcg tggctcatca tgagcgcgcc cggactggaa gcaatccctg
     1861 tggagattgg aatcagcggg gtcgggagtc aaaggaatcc cggcacattc gaccaggggc
     1921 tggacaatta cagaacatcc ctcaacccac tggtgttctc gcttacgaag cattcaaact
     1981 tcagaagtat ccctgcctcg gagggttaca ttcgtctggg agatctgata tcggaagaaa
     2041 gttacattgg ccttctctat gggagactct acactccccc gatttcagtg cggcagtttc
     2101 gtagtgatgc agtctacttc cagtccaact ggaatttctt cgtgcattac tttggtttta
     2161 cataccacta tcgctttcgc ccgatttcca gctctcgcat gtcctacgaa gtcggaggag
     2221 cgtatacttt tgtggcagaa cctacatgga gaacagacgg atactatttg agtccttacc
     2281 aattcagtcg ccttcagacc agccatcgcg cgggatacgg tagcatgcta cgccttgaag
     2341 gggcggtggt tcgtggcctg ggagatcact tcttcgttcg cgcgggcatc gcgcaatctt
     2401 actggtatat gggtagatat cagggcaacg taaattcgac gggaggttac tgggtgacat
     2461 tgcgaaacgg cggttttctg gctcttgctc cttctcagtt catttccacc ttcgtggtaa
     2521 gcccggatcc aattctcgga ccaacgcagg taagtacgat tcgggaccgg gcggacataa
     2581 atttcagtct aacccagttc tttgtcagtg cgggcctcag attttgattt acaacatccg
     2641 actctcctaa tatcccatgt cagggttccc atgagcgcag aaaaagtaat caccatgcca
     2701 gtacgtgaaa tcgcagatgt gaaggtcatt ctggccacct ggtataagtt tctgcaggat
     2761 caagcggaca agctaaacgc agaggaattc tctcggtcgc ttaagactcc catcattcta
     2821 aacctggaaa cggacgaact cgaactactc ttcaccggat ccggagaact ccttcagggg
     2881 ttcaaagatt tcatactcac tacaacctga gtgcttctca cagcttaaac aattcgggtt
     2941 cttcgtattt ttccaccagg cgtttgtcgt cttcgcgcaa cctggaatcc actttgtatg
     3001 tttccattct ccccgctgca aatccagtgg catgtttgcg aaacaaagag tctgatggtg
     3061 tgttagggtc taaccattcc gaaatttcat ttgcatcgag aatcacaggc attctatgct
     3121 tcgcatgaat cggacgaatc aactcatttg cctccgtagt caagatcgtg catgaaacga
     3181 tttctactcc tccgcctttc cacgtatccc agagaccggc catggccaga ggctttccat
     3241 ccaatcgacg aaaatagtat cgatcctttt tcttttttgc cacatccttc cattcgtaga
     3301 atccactcgc tggaatcacg caccgtcgat tcgcaattac actcgaccga aacgcaggtt
     3361 tagtggccgc ttcttccaac cgggcgttga aggtagcgta tctgacttga tttgatttct
     3421 cccaggccgg aagcagagcc catcgcatca gagacgctcc gtgatgcgca aaagacaaaa
     3481 cctgatctgt tggccgaatg tcgtagtgcg gcccgaaatc caaatcgatc tgaaaaccaa
     3541 aaaatccttc gagcacttct ttctcttcga aaagagcaaa tcgtccgcac atccgggaag
     3601 catgctctcg tggctttttt tcgcaacgat atttgaagtt agtcgaaacc tgttgaccca
     3661 tggaacaccg acccgcgtct aaggtgcatg agatctatcg tattcgctgt cctggttgcg
     3721 atcatagcgc cgctgatggc acaaacaaaa gcaaacgaca tccccgtcga gaagctgcct
     3781 gcagacgtaa aacaggttct cgaacagtat gtgaacatat tgcgcacgag caagagcctg
     3841 gacgaatgcg cgacccgctt cacttccatt gccggtggca gtctggttac ggatgaaaaa
     3901 ggaacccttc gcgatactgt gaaaccattt tccctgaaga aggatttcaa tgacatcaag
     3961 cagtacgcgg atccaattgt aatcactcgc gtgaatgcaa cgccgtccaa tggacagggg
     4021 tacggatcga gcgccatcaa aggcatggtg tacaaaattt ggatcgcgaa aaaggacgca
     4081 tccattggaa tgcctgcccc cgtatcgatt ctggttccag aagggcaccc caccatcacc
     4141 acacccaaag tggtcggaat tggaagttac tgacctccgg cgcccgaaag ggcgcctcga
     4201 ttcttaatcg ccggataacg ttatgtatca gctattcatt caaattatca aatacggttt
     4261 cagtactgtt ctgtttggag taggactcta cgtcttgatc gcatcctacc gcgagcacaa
     4321 atctttcaga cgcttgttca aaggattcgc gatcactgca tacttcttgt ttgccgcagg
     4381 aatgactctg ctaattttca caggcgaaac ggcagcacag ttgagcatgt tcttcatcaa
     4441 ggcaatctcc actgcgttcg gattgctgta cggcatttac gcaactctca acgggttcat
     4501 ggaaacggat ccggccaccg gaaagaagac cgttacgcga cttggggtcc tgggtatttt
     4561 catcctcagc tttaacacga tcctcctggt cagcatggat taccttcaag aaaccgccgc
     4621 catggaagaa aaactgcagg agaaacaact gcaactggac caggaacgcg aaaggaaaat
     4681 tgaatacgaa cgccttcttg ggagcgtcag tttcgtggat cgaaaaacag gcgatttgaa
     4741 aatcgacttt gagaagttta gcgaaagcct gtacagatcc ctggtcacag tggaaacaga
     4801 tctccgcaat accagaattg aactggcaac cagagaaaca gaattgaagg acgcaacggc
     4861 tcgatggaat gcagcagacg ccggcgcaaa agtcttacaa gaaactcttt cgaaaacgga
     4921 aaaggccctg gccactcgcg acgcggaatt aaaagagtcg cagactaaac tggcccagag
     4981 cgacgccagc ttgaaggccg cgcaagaaaa tcttgctaaa acagagaaga gcctcgcagg
     5041 acgagaagca gaactgaaaa atacttcaga tcaacttgtt gaatcgcaaa aggaagcaaa
     5101 gaaagcagaa gaagagctaa gccgaacgtc acgcgatctt gcaagcaaag atgcggaggc
     5161 acgtgaacaa actcgccggg ccactgccgc agaatcatcc cttgcgcgtg cgcaggatga
     5221 gttaaaaaag tccgctgcag cattcactac tcgcgatgca gaagcccgcc agtaccagaa
     5281 agagaattta actctacaaa caaatcttgc gcgcgaaacc gaagagcgca gtaaactgga
     5341 caagcttctt gcagcgcgcg acgcagaact gcgcgcatgc aaaacggcac cggcaactcc
     5401 aacgccacca cagcctgtgc caccgggcta atccaccgcg aacgatgtct actgttctac
     5461 gcacaagagc atatagcccg ctgtgttgca gttgttgcct gcggccgaaa tagacgcatt
     5521 tgaagtcacg tttgcgtttc caatcagagc agtggtcgca gttccggtcc aaccagaaca
     5581 atcggccgct ccgttag
//
LOCUS       DAORFF010000223         5590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7929_length_5590_cov_3.594038, whole genome shotgun sequence.
ACCESSION   DAORFF010000223 DAORFF010000000
VERSION     DAORFF010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7929_length_5590_cov_3.594038"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /gene="metG"
                     /locus_tag="PKK76_10050"
     CDS             <1..663
                     /gene="metG"
                     /locus_tag="PKK76_10050"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HNL68858.1"
                     /translation="ALNSARILAGLLKPVVPSFVRGVENLLNLKEEITFQNLDQKLPS
                     EHIIHPYRNLAGRMDEKVFDAMLEEEARLAAAEKPAASAEKTAPETGAKSQSKASEKP
                     AEKAGDQAKSGLISIEDLEKVELRAGRILHAELVEGADKLLRVELDVGEAEPRQVFAG
                     IRAAYKPEDLTGLTVACVANLIPRKMKFGISSAMLLASGKDAELTLYTAHRQAKPGDR
                     LK"
     gene            680..3658
                     /locus_tag="PKK76_10055"
     CDS             680..3658
                     /locus_tag="PKK76_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68859.1"
                     /translation="MRPLLRTLASVSPIVATAITFLLVSPLFAQITPGLGDWRTHSTP
                     HFRIHYQKADEELAHRMAGIAEKVHAKLDPKYQTGATETHVILVTSTDLVNAFATPVG
                     LDRVYLFLDHPRPGEFARFDLWAEILFTHEYTHILTLRYYNTPFLYTMRIILGFPPNL
                     IGPIGLQEGYAVYEESQKGKGRLHDPLTHMIVRTAVLEDRFPGAGEAMSGSHRWPHGQ
                     TYYLYGAMMAEYLHKTEPEKAGKFWREETPVWFLEMKLSSMRIRSFNLLYRDFRTSKE
                     EEMRKEIESLRAAGLTQYKLLTQDGETKEHLTEGETGLLYFARPGARASGMFRLIPGS
                     EAERIRLSYTSEGMTERNGQILASEDHFFYPGLGIRQELHDARSVLFSRLLPGVSVVD
                     PLFSPDGNTLYYIEKDRFERRLIRASWKNEEIKDPETIFHVPFSGILRFPAVSPDGQQ
                     LAFVARKDETGMGVVLVCDARASNCKTVAAGPAAKVRPRFSKDGKQLFFSSDADGIYN
                     IYSVNLASGEILKRTRTLTGFFDPVPTESALYALSYNGKGFDVAEISYEHMLAEKSGL
                     FAAHGDESSFFEAETEAPLPGQPQNWPEDPDYNGALSMRPYFAGLLGSGSLLGLGVAA
                     LDPLERHVLAAGIVAGAPDPYAWAFYDYSRFVVGFSGFYSTNYWKRDRAPGCLNPSDP
                     NRFICDDQYSFVESMGGYLRYTFEGRYVSFQVLPGYASTKMRNARRIRTLEMDARDLN
                     LSGPSFAMLLGRTEEFAESVSPERGFRFFALTDYYSKTTSRRELDPQTQRPIDYGAAE
                     GGLALYLPSFFDHHVNYLSGYAFTSYGPDREVEKVRLSRLQRGIAYDRSPTATSAVVW
                     TYEYRLPIAWLSASPESMPHFILRNIGMSLFYEAGAAFEKIPYQRDYKSSVGVTFQFG
                     ITALYLPLAPFRLTFVKGLGREGENQVYLGYETTFGPGMDSGRNIPGNRIVEPWHRPI
                     PRSTEEPGYFRRASAGGILE"
     gene            complement(3667..4734)
                     /locus_tag="PKK76_10060"
     CDS             complement(3667..4734)
                     /locus_tag="PKK76_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68860.1"
                     /translation="MAESLLKKQRPAKTPKQRVFVAALPGESDLPAFLEGLYATRLIR
                     MRHPDIRTMLIVAPANRALAEECGLFDEIIDAAGDLSTLIRNVHADIIYVPSVDFRLS
                     MKLLFSGSIRIAGGARLGKFLRFFDLRRDGEKLKKAGLDMLPESVGISFRNAPLPDEM
                     IVYASLFDEHNVSGSWPVGHAARLSRLLSTLGAKMIVPLPEEKFTRTFTPLPSSNSRE
                     FAKDVQYLKKYAPEIQFVSAKEIQKKAEWMRKSSAIVAPAGSETVFAALLHRPVVTLH
                     DMQSHRMLGKPDARSLGPRTADPGVLGLFTKIADSLDRHLMPQVEECIEDCPACTHLS
                     CVDTISPERVFENVKRILMPY"
     gene            complement(4727..5368)
                     /locus_tag="PKK76_10065"
     CDS             complement(4727..5368)
                     /locus_tag="PKK76_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNL68861.1"
                     /translation="MPIGHPSDITLRALESFRDANLIFVEDPSTVSRLFKKWDVPFIR
                     ERIRVLNEHTTADELNDLVREAANVELSVVVSDAGMPVFCDPGGDFMRLCEKAGIRIE
                     IVPGPTALTSALVRAGINGAFYFAGFPPRKSEERGGFFRKLNQMTVPIVLYETPYRLE
                     KILGEIQRHFRPDKKVIVCAGLTSPDESIVTSTVANAGKIRIQSAPPVIIIDG"
     gene            complement(5392..>5590)
                     /locus_tag="PKK76_10070"
     CDS             complement(5392..>5590)
                     /locus_tag="PKK76_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNL68862.1"
                     /translation="ITRELAYEILQKWPKDTKIAFYCHTGVRSLEAASYFKGHGLPNV
                     KSLRGGVDRWAVEIDTSLPRY"
BASE COUNT         1426 a         1426 c         1412 g         1326 t
ORIGIN      
        1 gcattgaact ctgcccgaat tctagcgggc ctactcaagc cagtcgtccc ttcctttgtt
       61 cggggcgtgg agaatttgct aaatctaaaa gaagaaatta ctttccagaa tctagaccag
      121 aagctccctt cggaacatat cattcatccc tacagaaatc tggcgggaag aatggacgaa
      181 aaggtatttg acgcaatgct ggaagaagaa gcaagacttg cggcggcaga aaagccggcc
      241 gcatccgcgg aaaaaacagc acctgaaaca ggcgctaaat cacagagcaa agcctctgaa
      301 aagcctgcgg aaaaagctgg ggaccaggca aagtcaggac tcatctccat agaagatctg
      361 gaaaaagtag aactgcgcgc gggaagaatt ttgcatgcag agctggtgga aggtgcagac
      421 aagttgctcc gcgtggaact ggacgtggga gaagcggaac cacgccaggt gtttgccgga
      481 attcgcgccg cttacaaacc agaagatcta actggactca cagttgcgtg cgttgccaat
      541 ctcattcctc gaaaaatgaa attcggaatc tcaagcgcca tgttactcgc ctccggaaag
      601 gacgcagaac tgactctcta tacggcccac cgccaggcaa agcctggcga tcgattgaag
      661 taatccggtg aaggagcaga tgcgcccact gctcagaacg ctcgcttctg tttcgccgat
      721 tgttgcgaca gcgatcactt tccttcttgt ttctcctctg ttcgcgcaga tcaccccggg
      781 tcttggagac tggcgcactc attcgactcc gcactttcgc atccactatc agaaagcaga
      841 cgaagaactt gcacatcgca tggcgggcat tgcagaaaaa gttcacgcga agctggatcc
      901 caaatatcaa acgggagcca cagaaaccca tgtgatactc gttacttcaa cagatcttgt
      961 gaatgctttt gccactccgg ttggtctcga cagagtttat ctattcctgg atcatccaag
     1021 acctggagaa tttgcgcgat ttgatttatg ggcagaaatt cttttcactc atgaatacac
     1081 gcacattcta acactccgct actacaacac gccgtttctg tacacaatgc gaattatact
     1141 gggctttcct cccaacctga tcggcccgat cggtttacag gaaggatacg cggtctacga
     1201 agaaagtcag aaagggaaag gtagactcca cgatccgctg actcatatga ttgttcgcac
     1261 ggcagtactc gaagaccggt tcccgggtgc tggcgaagca atgtctggct cacacagatg
     1321 gccgcatggg caaacttact acctctacgg cgccatgatg gcggaatatc ttcataagac
     1381 cgaaccagaa aaagccggaa aattttggag agaggaaaca cctgtctggt ttctggaaat
     1441 gaaactctcc tcgatgcgta ttcgctcttt caacctgctc taccgtgatt ttcgcacttc
     1501 aaaagaagaa gagatgcgca aagaaattga atcgctcagg gcagcaggtc ttacgcagta
     1561 caaactatta acgcaggacg gagaaacaaa ggaacacctg acagaaggag aaacgggact
     1621 tctgtacttc gcgcgtcccg gcgctcgtgc atccggcatg tttcgattga ttccggggtc
     1681 agaagcggag agaatccgtc tttcctatac gtcggaggga atgacggaaa gaaacggcca
     1741 gattctcgca agcgaagacc atttctttta ccccgggctt ggaatcagac aggagcttca
     1801 tgacgccaga agtgttctgt tttcgcgact cctccccggc gtgagcgtag tggatcccct
     1861 tttctctcca gacggaaata ctttgtacta catagaaaag gatcgctttg aaaggcgact
     1921 gatccgcgcc agctggaaaa atgaagaaat aaaggatcca gaaaccatat ttcatgttcc
     1981 tttttctggt attctgcgtt tccctgcggt ttctccggat gggcagcaac ttgcttttgt
     2041 ggccagaaaa gacgaaacag ggatgggagt ggtgcttgta tgcgatgcaa gggcatccaa
     2101 ctgcaagact gtggccgccg gtcccgcagc aaaagttcgc cccagatttt ctaaggatgg
     2161 aaaacaactt ttcttctctt cagatgctga cggaatctat aacatctact ccgtaaacct
     2221 cgcgtcagga gaaattctga aacgcactcg cacactcact ggcttctttg atccagtgcc
     2281 taccgaatct gcgctgtatg cattgtcgta taacggtaag gggtttgatg ttgccgagat
     2341 ttcgtacgaa cacatgcttg cggaaaaatc tggattgttt gccgctcatg gagacgaatc
     2401 gtcattcttt gaagcagaaa cagaagcacc tctacccgga caaccgcaga actggccgga
     2461 agatccggat tacaacggag cactttccat gcgcccttac ttcgccggct tgcttggaag
     2521 cggcagccta ctggggctcg gggtggcggc gcttgatccg ctcgagcggc atgttctggc
     2581 agcaggtatt gtggccggtg cgcctgaccc gtatgcctgg gccttctacg attactcacg
     2641 gttcgttgta ggattttccg gcttttattc tacaaactat tggaagagag atcgcgcgcc
     2701 cggatgcttg aatccatcag atccaaatcg tttcatttgt gacgatcagt attcttttgt
     2761 cgagagtatg ggaggatacc tgcgatatac tttcgagggc aggtatgttt ccttccaggt
     2821 cctacctgga tatgcatcca ccaaaatgcg aaacgcgcga aggattcgga cgttggaaat
     2881 ggacgcgcgt gacttgaatc tatccggtcc ttcatttgct atgcttctgg gcagaacaga
     2941 agagtttgca gaatcagtga gcccggaaag gggctttcgc ttttttgcac tcacagatta
     3001 ctactccaaa acaacttcgc gcagagaact cgatccgcaa acacagcgac ccattgacta
     3061 cggtgcagcg gagggcgggc ttgccctcta tcttccgtct ttctttgatc atcacgtaaa
     3121 ctatcttagc ggttatgcat tcacatctta cgggccagat cgcgaggtag agaaagtcag
     3181 actgagcaga ctgcaacgcg ggatcgccta tgaccgctcc ccgaccgcga cgagcgccgt
     3241 tgtatggaca tatgaatatc gtctaccaat tgcatggctt tctgcgtctc ctgaatctat
     3301 gccgcacttc atactacgca atattggaat gtctctgttt tatgaggcgg gcgccgcctt
     3361 tgagaaaatt ccataccaga gggactacaa aagcagcgtg ggcgtgacct tccagtttgg
     3421 aatcacagca ctctaccttc ctctcgctcc attccgccta acgttcgtca aaggtcttgg
     3481 ccgcgaggga gaaaaccagg tctatcttgg ttacgaaaca acgtttggtc ctggtatgga
     3541 cagcggacgc aatattccgg ggaatcgaat tgtggaaccc tggcatagac caattccgcg
     3601 aagcacagaa gaaccaggat atttccgccg cgccagcgcc ggcggcatac tcgaatagct
     3661 agcgtctcag taaggcatca aaattcgttt tacgttttcg aaaactcttt ccggcgaaat
     3721 tgtatccacg caggatagat gcgtgcaggc cgggcaatct tcaatgcatt cctctacttg
     3781 aggcatgaga tgcctgtcca gagagtctgc gatttttgta aacaaaccca gcactcctgg
     3841 atccgcagtt ctcgggccca gagatctggc gtcgggtttg ccgagcattc tatgcgattg
     3901 catgtcgtgt aacgttacaa ccgggcgatg caaaagtgct gcaaacacag tctcggaccc
     3961 ggccggcgcc acaatcgcag aagattttct catccattct gctttctttt gaatttcctt
     4021 cgcagatacg aactgaattt cgggtgcata cttcttgaga tactgaacgt cttttgcaaa
     4081 ctcacggctg ttcgaagaag ggagcggagt aaatgttcga gtgaattttt cttccgggag
     4141 tggaacgatc atcttcgccc cgagcgtaga aagcaggcga gaaaggcgcg ctgcatgacc
     4201 cacaggccag ctaccggaaa cattgtgctc atcaaagagg ctggcataca caatcatctc
     4261 gtcagggagc ggggcattgc gaaatgaaat tccaacggat tccggaagca tgtccagccc
     4321 ggccttcttt aacttttcgc cgtcgcggcg aagatcaaag aaacgcagga attttccaag
     4381 gcgagcgcct cctgcaatgc gaatggatcc ggaaaacagc agcttcatgc tcaagcgaaa
     4441 atcaaccgaa ggaacataga taatgtccgc gtgaacgttc cgtatcaaag tggacagatc
     4501 ccccgcggca tcgatgatct catcaaatag cccgcattct tctgcaagtg cacgattcgc
     4561 aggagcaaca atgagcatcg tgcgaatgtc aggatgacgc atgcgtatca gtcgagtggc
     4621 gtagagccct tctagaaacg caggtaggtc gctttcgccc gggagcgccg ccacgaacac
     4681 tctctgcttt ggtgttttag ccggacgctg tttttttaga agagattcag ccatcaatga
     4741 tgattaccgg aggtgctgac tggatgcgaa tcttacccgc atttgctact gtcgaggtga
     4801 cgatactttc atctggcgat gtcaaaccgg cgcagacaat cactttttta tccggcctga
     4861 agtgtctctg gatttcaccg aggatctttt ccagacgata cggagtttcg tacaatacga
     4921 ttggtaccgt catctgattc agttttcgga agaatccgcc tctctcctct gattttcttg
     4981 gagggaagcc tgcgaagtag aaagctccat tgattcccgc tcggacaaga gcgcttgtca
     5041 gagccgtagg accaggaacg atttcgattc gaatgcccgc cttttcgcac aggcgcatga
     5101 agtctccgcc cggatcacaa aacacgggca tgcccgcgtc tgaaacaacc acagagagtt
     5161 ctacgtttgc cgcttcccgg accaggtcat ttaattcatc cgctgtggtg tgttcgttga
     5221 gtacgcgaat gcgttcgcga atgaaaggca cgtcccattt tttgaaaaga cgcgaaactg
     5281 tagaaggatc ttcgacgaag atcaaattcg catcacgaaa ggactcaaga gcgcgaaggg
     5341 taatgtcaga gggatgcccg atgggcatcc ctgtaactct aagtgtcgag atcagtatct
     5401 aggaaggctt gtatcgatct caaccgccca gcgatctact ccaccgcgca gactcttgac
     5461 attgggaagt ccatgtcctt tgaaatacga cgcagcttcc agagagcgaa ctccagtatg
     5521 acagtagaat gcaatcttcg tgtctttggg ccacttttga agaatttcgt aggccagttc
     5581 ccgtgtgatg
//
LOCUS       DAORFF010000224         5574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7968_length_5574_cov_3.911759, whole genome shotgun sequence.
ACCESSION   DAORFF010000224 DAORFF010000000
VERSION     DAORFF010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7968_length_5574_cov_3.911759"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PKK76_10075"
     CDS             complement(<1..279)
                     /locus_tag="PKK76_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL68863.1"
                     /translation="MSKQEHGKYGESQITALKGLEAVRKRPGMYIGTQDATGLHKMVY
                     EVVDNSVDEAMAGYAKHIIVRMLPDGVIEVQDDGRGIPTGIHPTEKIST"
     gene            complement(279..626)
                     /locus_tag="PKK76_10080"
     CDS             complement(279..626)
                     /locus_tag="PKK76_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNL68864.1"
                     /translation="MDKAKREKFKEKLLKAKAEIIKELEVEQEYFVYNDQGDLVDVAD
                     SVISNEILNKLSDMDLDKVRLIDIALEKMEKGTYGVCEGTGKNIPEARLNAIPWTRYT
                     VEYAEQMEKLKAN"
     gene            836..1222
                     /locus_tag="PKK76_10085"
     CDS             836..1222
                     /locus_tag="PKK76_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68865.1"
                     /translation="MIEKNLQDVLNAGIGLFKSSEENFKVAVSNLEKTFNDMRNKGAA
                     DNSDAAIKIREILENTLRSAKDVQAKAEQNLNVFMAEAQKNYSQVLEQVKKVVGEERI
                     RDLNTKIDELTTLVKQKVGQPGGPKV"
     gene            complement(1289..2629)
                     /locus_tag="PKK76_10090"
     CDS             complement(1289..2629)
                     /locus_tag="PKK76_10090"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL68866.1"
                     /translation="MSTPAADVNSITEFRPYLNAIIHSWLKIMTALGYTLVPLFFVLD
                     IFVIPKEHSDLLPRFAIYRAIVTAFIIAQSIVIHRTKPSNYSFIHGYVFTVSVSMVIV
                     MMTRDLGGFNAPYYAGLNLVIIAANMLMPWHAKHSAINGLLTVGMYVLVNSFFGGPFT
                     WNILINNLYFLFGTVIIVAAINFVKHNLIKKEFQGRQELRLARDALWGEMEIAKRIQT
                     ALLPPDHGISGYEIAAVMVPADEVGGDYYDILDFGDKKWITIGDVSGHGVESGLIMMM
                     TQTSVKSMVSNDPMASPAAVLSHVNTVLKENISRLGADRYMTISALSLNGKTMVVAGK
                     HQDIFIYRKKSRHIETVHTHGAWIGILDDITDFLTDQEVALDVGDIILLFTDGITEAE
                     NKEGDMFGEERLNDKLNEYANLPVKEIMNNILRDVTVFQERQADDITLLIIKKI"
     gene            complement(2672..4018)
                     /locus_tag="PKK76_10095"
     CDS             complement(2672..4018)
                     /locus_tag="PKK76_10095"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL68867.1"
                     /translation="MKFLKRFIVPNPQLTPEHEALFHEETLSSARNSLQFVCFAAGTL
                     YPIFGVLDYISFPDRYLGLVFIRCIVVPFVFLTLGLTFVDRLRPYQNWFGIGLFQVLA
                     LGIIAMTWFTGGKTSPYYAGIILVLLTQIVAMPWRLRMVFANSFGVWGIYTISVILLD
                     WINEANSSEADWRLFIGNNFFLIGTILIGSVWSGISFRLRKVVFLNRAQLALEKEKSE
                     ELLRNTLPDFVIEELKRNGSVHPRRLDCTVLFTDFAGFTKISEKMEPEDVLLELDRSF
                     EYFDTICEKFGMDKIKTIGDSYMCAAGVDAAGKRHAIVAALTAIEFRSYMNMMKQIKM
                     AVNEEYWQIRIGIHSGPVVGGIVGKFRYAYDIWGDTVNTASRMESSGIIGEINISQDT
                     NSLLQEYFVTHSRGLVNAKGKGELEMFTLDSIRPEFADDEAGRTPNAKLLSLIDIS"
     gene            complement(4015..5406)
                     /locus_tag="PKK76_10100"
     CDS             complement(4015..5406)
                     /locus_tag="PKK76_10100"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL68868.1"
                     /translation="MSAATTFEGELIGKERRIEVTVGYGSRYSLLATIQNNQLIPYNE
                     QFDELSLKANGSNISFGPCFLITDDNIGKFNGRIVFAHDAYNFDSFFQNTAAPGLEGH
                     FHNLPLIRSQKDRIEPLFKEYTQNLLYDLNVHKTFFDDLDRKLQLETDSVRRAAQNAI
                     IETEGRKFLRFFDQSIDDLQKIVKNFSKEENERHGFYFRRMVWHLILTSDFLARTNIK
                     PRGYQGDSEMMRMTYVNDYEGSSIFGKLLHKHPIETAAAQAVRNRRKYIPEILHETLG
                     HFPKTGKHAFRIMSIACGPAWEMQDVFAGSDSFDRFHLTLLDQDVEALGEAASAISAV
                     EKARGLKTRVDYLNESVRTMLRTPKLQDKWGQHHFIYSMGLFDYLTGPVAKAVIKKIF
                     ELLKPGGTMIIGNYHVDNPTKTYMDYWMDWVLFYRTETEILSLLADEPKAEASVTFED
                     SRSQMFLHVRKKA"
BASE COUNT         1436 a         1387 c         1323 g         1428 t
ORIGIN      
        1 cgtagagatc ttttctgttg ggtgaatccc ggttggaatg ccgcggccat catcctgtac
       61 ttcgatgacg ccatccggaa gcatgcgcac gatgatgtgt ttggcgtaac cggccatggc
      121 ctcatctact gagttatcta caacttcgta gaccatcttg tgcaggcccg ttgcgtcctg
      181 cgttccgatg tacatccccg gtcttttgcg gaccgcttct agacctttta gcgctgtgat
      241 ctggctttcg ccgtacttac cgtgctcctg ctttgacatc aattagcctt cagtttctcc
      301 atttgctctg catattccac tgtgtagcga gtccatggaa tcgcattgag tcttgcttca
      361 ggaatattct ttccggtgcc ttcacaaacg ccgtacgtgc ccttttccat cttctcaaga
      421 gcaatgtcta tgagccgaac tttgtccagg tccatgtctg aaagtttatt tagtatttcg
      481 ttgctgatga cgctgtccgc cacgtcgacc aggtctccct gatcgttgta tacgaagtac
      541 tcctgctcta cctcaagctc tttgatgatc tcagccttgg ccttgagcag cttctctttg
      601 aatttttcgc gctttgcctt atccatacgg aattatgtcc accagaacta gtaaaggcac
      661 aatcctgccg gccctgggct ccctgtaaag aaaaaaggga tcatgggccc ggatggcctc
      721 tcccgtaccg aaaaaatgca cctcgggatc gttttttact tgattttgcg gcgcactaaa
      781 tcaattgtgc actgcataag gacatccaag gatgtccggc aaatcggaga aaaccatgat
      841 cgaaaaaaac cttcaagacg tactgaacgc cggcatcggc cttttcaaat ccagcgaaga
      901 gaacttcaaa gtggcagtgt ccaatctgga gaaaaccttc aatgatatga gaaacaaagg
      961 cgccgctgat aactcagacg cagcgatcaa gatccgtgag atccttgaaa acaccctgcg
     1021 tagcgcaaaa gacgtccagg cgaaggcaga acagaacctc aatgtgttca tggcagaagc
     1081 ccagaagaac tacagtcagg ttctcgagca ggtaaagaaa gtggtaggcg aggaaagaat
     1141 ccgcgacctc aacacaaaaa tcgatgagct tacaacgctc gtaaaacaaa aagtcggcca
     1201 gcctggcgga ccaaaagttt aattagcgtt ctaaacgaat gcgaagagca gccgggcaat
     1261 agcccggctt ttttttgccc gctggttgct agatcttctt gatgatcaac agagtgatat
     1321 cgtctgcctg acgttcctgg aaaacagtta catcgcgcag aatgttgttc atgatttctt
     1381 tgacaggaag gtttgcatat tcattcagct tgtcgttcag acgttcttcg ccgaacatat
     1441 cgccttcttt gttttctgct tctgtaattc catctgtgaa aagtagaatg atatctccca
     1501 catccagagc cacttcctga tccgtaagga aatcagtaat gtcgtccaga attccaatcc
     1561 atgcaccgtg tgtgtgtact gtctcaatgt gcctggactt tttgcgataa atgaaaatgt
     1621 cctgatgctt tcctgccact accattgtct ttccgttcaa ggaaagagca gaaatggtca
     1681 tgtaacgatc cgcacccaga cgcgatatgt tctccttgag aacagtattc acgtgactga
     1741 gaacagccgc cggacttgcc atgggatcat tgcttaccat ggacttcaca ctggtttgcg
     1801 tcatcatcat gatgagacct gattccactc catgaccgga cacgtctcca attgtgatcc
     1861 acttcttatc cccgaagtcg agaatgtcgt aataatctcc gcccacttca tctgcaggaa
     1921 ccatgactgc tgcaatttcg tatcctgaga tgccatgatc cggaggaagg agtgccgtct
     1981 ggattcgctt ggcgatttcc atctctcccc acaacgcatc tcttgccagg cgcagttcct
     2041 gacgtccctg aaactctttc ttgatgaggt tgtgctttac gaagttgatc gcggcgacaa
     2101 tgatcactgt cccaaagaga aagtaaagat tgtttatcaa tatgttccag gtaaacgggc
     2161 caccgaagaa ggaattgacc agcacataca tgcccactgt cagaagtccg ttgatggcag
     2221 aatgctttgc atgccagggc atgagcatgt tggctgcaat gatgaccaga ttgaggcccg
     2281 cgtaatacgg cgcattgaaa ccgcccaggt cgcgagtcat catcacgatg accatactga
     2341 cagagacagt aaagacgtag ccatggatga acgaatagtt agaaggcttt gtgcgatgaa
     2401 tgacgatcga ttgcgcgatg atgaacgctg tgacaatggc gcgatagatc gcaaagcgcg
     2461 gcagaaggtc cgaatgctct ttgggaatga caaatatgtc caggacaaag aacagaggca
     2521 ccagggtgta gcccagcgca gtcatgatct tgagccacga atggatgatt gcgttaagat
     2581 agggcctgaa ctcggtaatc gagttcacat cggctgccgg cgtgctcatg tttcagatat
     2641 cgtccccctg cagggcagga tgaaggggct ctcaggaaat gtcaatcaac gataagagct
     2701 tcgcattggg ggttcggccg gcttcgtcgt ccgcgaattc cgggcgaatc gagtccaggg
     2761 tgaacatctc cagctccccc ttccccttcg cattgaccag ccccctggag tgggttacga
     2821 agtattcctg caggagagag ttggtatcct gcgaaatgtt gatttcacct ataattccag
     2881 agctttccat gcgcgatgcg gtgttcaccg tatcccccca gatgtcgtag gcataccgga
     2941 acttacctac gatgcccccc acgaccgggc cggaatgaat gccgattcga atttgccagt
     3001 attcttcatt cacggccatc ttgatctgct tcatcatatt catgtagctg cgaaactcaa
     3061 tggcagtgag ggcagccaca atcgcatggc gctttcctgc cgcatccaca ccggccgcgc
     3121 acatataaga gtccccgatc gtcttgatct tatccattcc aaacttttca cagatggtat
     3181 cgaagtattc aaaggaccga tcgagttcga gaagaacgtc ttcgggttcc atcttctctg
     3241 aaatctttgt gaaacccgcg aagtcagtaa acaggacagt gcaatccagt cttcttggat
     3301 gaacacttcc gtttctcttc aactcttcga tcacaaaatc tggaagagta tttcgcagaa
     3361 gctcttcgct tttttctttt tccagagcaa gttgcgctcg gttgagaaac acaaccttgc
     3421 gcaggcgaaa gctgatgccg gaccagacag atccaatgag gattgtcccg atcaaaaaga
     3481 agttgttccc aatgaagagc cgccagtctg cttcgctcga attggcttca ttgatccagt
     3541 ctagcaagat cacagaaata gtataaatgc cccagactcc aaagctgttg gcgaaaacca
     3601 tgcgaagtct ccatggcatg gcgacgatct gagtgagaag gacaagaatg atgcccgcat
     3661 agtatggact tgtttttcca cccgtaaacc aggtcatcgc aatgatgccc agggccagaa
     3721 cctgaaacaa tccaatccca aaccagttct ggtaagggcg aagacgatcg acgaacgtta
     3781 aaccaagagt cagaaaaaca aacggaacta cgatgcagcg aatgaatacg agacccaggt
     3841 atcgatcggg aaatgaaatg tagtcgagta caccgaaaat gggatagagc gttcccgcgg
     3901 caaagcaaac aaactgaagg gaattcctcg cgctactcag agtctcttcg tgaaaaagag
     3961 cttcatgctc tggcgtaagc tgtggatttg gaacgatgaa tcttttgaga aacttcatgc
     4021 tttcttgcga acatggagaa acatctgact tcttgaatct tcaaatgtga cggatgcttc
     4081 cgcttttggc tcatccgcaa gcaaagatag aatttcagtt tccgttctat agaagagaac
     4141 ccagtccatc cagtagtcca tgtaggtttt ggttggattg tccacatggt aattcccaat
     4201 gatcatggtc ccgcctggct tcagcaattc gaagatcttc ttgatcaccg cttttgccac
     4261 agggccggtc aagtagtcaa agaggcccat agaatagatg aaatgatgct ggccccattt
     4321 gtcctgaagc tttggcgtgc gaagcatggt tcgcacagat tcattcaaat aatccacgcg
     4381 agtcttgaga cctcgagctt tctccacagc agaaatggca gaggccgctt ctccgagagc
     4441 ttccacatcc tgatccagga gagtcaggtg aaaacggtca aagctgtctg atccagcgaa
     4501 aacatcctgc atttcccatg cagggccgca tgcaatcgac atgattctaa aagcgtgctt
     4561 cccggttttt gggaaatgtc ccagagtttc atggagaatt tcaggtatat atttgcgacg
     4621 attgcgcact gcctgcgcgg ctgccgtctc aatcgggtgc ttgtgcagga gtttgccaaa
     4681 aatggagctg ccttcgtagt cattgacata cgtcatgcgc atcatctctg aatcgccctg
     4741 gtatccacga ggtttgatgt tggttcgagc cagaaagtca gacgtcaaaa tcagatgcca
     4801 gaccatgcgg cgaaaataga agccatgccg ctcattctct tctttggaaa aattcttcac
     4861 gatcttctgt agatcatcaa tgctttgatc aaagaaacgc aggaacttgc gcccttctgt
     4921 ttctatgatt gcattttgcg ccgcgcgcct gacggaatca gtttcaagct gaagcttacg
     4981 atcgagatca tcaaagaacg ttttatgcac gttcaaatca tagagcaagt tctgagtata
     5041 ttctttgaag agaggttcaa ttcgatcttt ctgcgaacga atcagaggaa gattgtgaaa
     5101 atgaccttcg aggccgggag ccgcagtgtt ctggaaaaaa gaatcaaagt tgtacgcatc
     5161 gtgtgcgaat acaatcctgc cgttgaattt tccaatgttg tcatccgtga tcagaaagca
     5221 cggaccgaat gatatattgc tgccgttcgc cttcaggctc aactcatcga attgttcgtt
     5281 gtacgggatg agctggttat tctgaatggt agcaagcaac gaataccggg agccatagcc
     5341 cacggtgact tcaatgcggc gttctttgcc aatcaattcg ccttcgaagg ttgttgcagc
     5401 ggacattgac cttacacacc ctcaatagag atctgaccgt cttcagtttt gaaaatggtc
     5461 agcgcgctga agctcaggtc ctgccacttg agcgcctttt tgtagagaat cttgatcttc
     5521 tgcttgttct tcttcgcagt gtccaggatc cgaatgatcg gagtgatggt ggac
//
LOCUS       DAORFF010000225         5570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7980_length_5570_cov_3.857842, whole genome shotgun sequence.
ACCESSION   DAORFF010000225 DAORFF010000000
VERSION     DAORFF010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7980_length_5570_cov_3.857842"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..925)
                     /locus_tag="PKK76_10105"
     CDS             complement(101..925)
                     /locus_tag="PKK76_10105"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNL68869.1"
                     /translation="MQKRHIAIAPILLALGLIAFKYFTAEKVVNPETGKEARVALSKE
                     EESVLGLQSYKEVLSKSKVVEEGAEVDQVRRVARRLAAATGEGGKGFKWEVSVVDSKE
                     VNAFCLPGGKIVVYTGILPVTKTDAGLAAVMGHEMAHATAHHGAQRLFQDDIYQTAMN
                     GAQQSIADLDPEKQRAVMAALGAGAKFGVILPFSRDHETEADEMGILYMARAGYDPRE
                     SVKFWERMEKMSGGQPPEFSSTHPSHGTRIARLKKFMPKAMEAYEKSDKQASNDSN"
     gene            complement(956..2092)
                     /locus_tag="PKK76_10110"
     CDS             complement(956..2092)
                     /locus_tag="PKK76_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68870.1"
                     /translation="MQEHRRFTDRGWNTIIADSVIVHRFLNPVEFPAACDPAEKKPEL
                     SLDPDAERVAYRCNVSAPWHIYFLQKSGSKAFELCATPAHLDWASVPSFEKARVSFLE
                     CESIQNNIFANTFKEIVAESRSRGGSASVADFLRDTISTSFASVFDPSGDDWDRAFRD
                     LPASERAKLQPFLKKTLEAPYNPYAVHRVVASMELSDDLDDLMVRAGEQMIASRPSGT
                     EDPLITGMRAQTFDVVVRQLGRTNPAAAARIACKDAEAFSRNGISIVIPRAQLAAIAR
                     QKIPCDIGGVLDRSKCDGGFACCYRGFTCGDSVRHACTAAELSELVKMELAQDPKKPW
                     PSGGGFGYELLVAVAMNQNSESARQFLERVKKVPVCDAPDSKKP"
     gene            2316..2900
                     /locus_tag="PKK76_10115"
     CDS             2316..2900
                     /locus_tag="PKK76_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68871.1"
                     /translation="MAHIQTNNRYPGILELLWYKPSTGQKLSALAHELLLGPSTLSSG
                     ERETIAAYTSALNDCEFCCESHNASACAHLGKDRPASVYLTPEFLAEQTPKMKALLEI
                     AALTQKGGRNVKPENVEAARKAGASDDEIHDAVLIAAAFCMYNRYVDGLGTRPPANPA
                     DYKEMGKRLSTQGYKFPPRFLYWLVRKILSKKYP"
     gene            2953..3966
                     /locus_tag="PKK76_10120"
     CDS             2953..3966
                     /locus_tag="PKK76_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HNL68872.1"
                     /translation="MNLESEIKQVIALCDKNGNLNPSAIGWSRKPLHRCNLSKHYFRK
                     KKWNYWCIYDKEWLISFTISDVDYAGLIFGYYLEFSTGKFAEFTKIVPFSSGITMGQQ
                     TDNNARYQSGGLSFDFQAKKHGQMLLASIPDIGGENLNASIDVEIPEDLESMNVVIPW
                     SKRRFQFTSKQIGLAASGTVTIGTRSHTFNKADTFACLDFGRGVWPYNITWNWGVAVS
                     RSEGEIVGANMGAKWTDNSGYTENSILAGGKTYKLPENVLFEYDRKDFMKPWKIRTEQ
                     SSAIDFEFTPFFDRHAKSNLVILASDVHQMIGKYTGEIKIGRKTVAIHDAIGWAEDHI
                     ARW"
     gene            4061..5299
                     /locus_tag="PKK76_10125"
     CDS             4061..5299
                     /locus_tag="PKK76_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LruC domain-containing protein"
                     /protein_id="HNL68873.1"
                     /translation="MKRILLLCMLAFSSCTREDPNYVWLLSLAAGGQTPPSGETSVTV
                     DVSDVTGSPDFLFDTTRTIPVFITVRDPVPQGSGTLVQILDSSSAQELLFQATSNAQG
                     SVEGTFTVNQTTKLVTLRVTIGTDVHTWTINITDVQEIRRFLFVTGEVNMTLVADADS
                     DGVPDSQDAYPLDATRSAIIRVPAQGFYTVAFEDLYPVQGDADFNDYVAEVRYEQDLD
                     ASGRVARMRGFVRHIARGAGYRHTLRLTLPTTSSSFHSKLFAANGSLELENETALTSF
                     EGIEVMPNSGTTISSANSAAGEVYAPGKSSEFEVIPATPMALASLGNAPYDLHLHVVN
                     TGYDIHFLGRYLDANGHDQYLDPSGFPWAIMIPGNWSWPLERVNMHTAYPKFQEWYQS
                     AGQAQTDWYLTPAAGKVFSK"
     gene            5359..>5570
                     /locus_tag="PKK76_10130"
     CDS             5359..>5570
                     /locus_tag="PKK76_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68874.1"
                     /translation="MKLEDRYQSLFEKAPLGFFVADRQGNIEATNSTLRSLFSLEKKK
                     KPNLLTERHLANAGFATQFRLCISRA"
BASE COUNT         1420 a         1487 c         1353 g         1310 t
ORIGIN      
        1 ccgccagtgc aggagttcgt ccgccacggg caatgcctca atatcgctta acgtcttgag
       61 cagacgcatt cttccatatt cgaactgatg gatcaacgca tcaattggag tcattgctcg
      121 cctgtttgtc tgacttttcg tatgcttcca ttgcttttgg catgaacttc ttcagacgcg
      181 cgatgcgagt cccgtgcgat ggatgagtag aagaaaattc cggaggttga ccgccgctca
      241 tcttctccat gcgttcccag aacttcacgc tttcgcgtgg atcataccct gcccttgcca
      301 tgtacagaat tcccatttcg tctgcttccg tttcatgatc gcggctaaaa ggaagaatca
      361 caccgaactt ggcgcccgct ccgagtgccg ccatcacagc acgttgtttt tctggatcca
      421 gatccgcaat ggattgctgc gctccgttca tagcagtctg gtaaatgtca tcctgaaaaa
      481 gtctttgcgc accatggtgt gctgttgcgt gcgccatctc atgacccata actgccgcca
      541 gtccggcgtc cgtttttgta accggaagaa tccctgtgta gactacaatc ttgccgccgg
      601 gcaaacagaa tgcgttgact tctttgctat ctacaacgct gacttcccac ttaaaacctt
      661 ttccgccctc tcccgtggct gcagccagac gccgagcgac gcgacgcacc tggtccactt
      721 ccgctccttc ttccactact ttggactttg aaagcacttc tttgtatgat tgcagaccaa
      781 gaacactctc ttcctctttg ctcagagcaa cgcgggcttc tttgcctgtc tctggattga
      841 cgactttctc tgctgtgaag tatttgaatg cgatcaaacc cagcgcaaga aggatgggtg
      901 cgattgctat gtggcgcttt tgcatgaact gagcgaagct aaacgccagg attgatcaag
      961 gcttttttga atctggcgcg tcgcaaacgg ggaccttctt cacccgctcg aggaattgcc
     1021 tggcagattc actattctga ttcatagcca ctgcaaccag gagttcatac ccgaacccac
     1081 caccggatgg ccagggtttt ttagggtcct gggccagttc catcttcaca agttcggata
     1141 gttccgcagc cgtgcatgcg tgcctcaccg aatcgccgca ggtaaacccc ctgtagcaac
     1201 aagcgaaacc gccgtcgcat ttgcttctat ccaatacgcc cccgatgtcg catggaattt
     1261 tttgacgcgc tatggccgcc agttgcgcgc gaggaatcac aatggagatt ccatttcgag
     1321 aaaaagcttc cgcgtcctta caggcaattc ttgctgccgc cgctggattc gtccgtccga
     1381 gctgacggac taccacgtca aacgtctgcg cacgcattcc tgtgatcaaa ggatcctctg
     1441 tgcctgaagg tctgcttgcg atcatctgct ctcccgcacg aaccatcaaa tcatccaggt
     1501 cgtctgaaag ctccatagat gcgacaacac gatgaaccgc ataaggattg taaggtgctt
     1561 caagagtctt ctttagaaaa ggctgcaatt tcgcgcgttc gcttgctggt aaatcccgga
     1621 atgcccgatc ccagtcatcg ccagatggat caaacacact tgcgaacgag gtcgagatcg
     1681 tatcgcgcag aaaatctgcc acactggcgc taccgccgcg agaacgcgat tcagccacga
     1741 tttctttgaa ggtattagca aagatgttgt tctggatgga ctcgcattcc agaaaagaca
     1801 cgcgcgcctt ttcgaaagat ggaacagaag cccagtccag gtgagcgggc gttgcgcaca
     1861 gctcaaatgc tttgcttcca gatttctgca aaaagtaaat atgccagggc gcagatacgt
     1921 tgcaccgata cgcgactcgt tcagcatctg gatccaatga aagctccggc tttttctcag
     1981 cgggatcgca ggcagcagga aattcgaccg ggttaaggaa acggtgcaca atcactgaat
     2041 cagcaatgat cgtgttccat ccgcgatccg tgaatcttct atgctcttgc atgacaattt
     2101 ctgcggatga ttttgtttct gtatacgcaa ccctggtttc cttactccca ccgccgcagc
     2161 ctgtgattgc aaataacagg aaggagatgg aaccaaacga aacgggaaaa acggaatgaa
     2221 gcggcgctct catcgcgcaa agtagaaggc acggaaacag atgggtcaag ataatttctg
     2281 acgacaaatg cggactctat ttgaatctcc caaccatggc gcatattcaa acaaacaaca
     2341 gatatcctgg tatcctcgaa ttgctctggt acaagccatc caccggacag aagctttctg
     2401 cgctggcgca cgaactcctg cttggtccat ccacgctctc ctccggcgaa cgcgaaacaa
     2461 ttgctgcata cacgtctgcc cttaacgatt gcgaattctg ctgcgaatcg cacaacgcaa
     2521 gcgcctgtgc tcatctgggg aaagacagac cagcatcggt gtatcttacc ccggagtttc
     2581 tagcggaaca aactcctaaa atgaaagcac tccttgaaat cgcagcgctt acacagaagg
     2641 gcggaagaaa cgtgaaacca gagaatgtgg aggccgcgcg aaaggcaggc gcaagcgatg
     2701 atgaaattca cgatgcagtt ctgatcgccg ctgcgttctg tatgtacaat agatacgtcg
     2761 acggactggg aaccaggcca ccggccaatc ccgccgacta caaggaaatg ggaaaacgtc
     2821 tgtccactca gggatacaaa tttcctccac gatttctcta ctggctcgtc agaaagattc
     2881 ttagcaaaaa gtatccctga gctaaaatca tttttcgttt tactgccagc ctggaatgat
     2941 cccggctgac gtatgaatct ggaatcagaa atcaagcagg tcatcgctct gtgcgataag
     3001 aacggaaacc tcaatccttc cgctattggc tggtcacgca aaccgttgca tcgttgcaac
     3061 ctgagcaaac actactttcg caagaagaag tggaactact ggtgtattta tgacaaagaa
     3121 tggttgattt cttttactat ttcagatgtg gactacgcag gcctgatctt tggttactat
     3181 cttgaattct ctacgggaaa attcgcagag ttcaccaaga ttgttccgtt ttcgagcgga
     3241 atcacaatgg gccagcagac agacaacaat gcacgctatc aatcaggcgg attgtctttc
     3301 gacttccagg caaaaaagca cggtcaaatg ctactcgcat ccataccaga catcggcgga
     3361 gaaaatttga acgcctccat tgatgtggag attccagaag atctagaaag catgaacgta
     3421 gtcattccct ggagcaaaag acgctttcaa ttcacttcaa aacaaattgg cctcgcggcg
     3481 agcgggacag tgaccatagg gaccagatcg cacacgttca acaaagcgga cacctttgcc
     3541 tgcctggact ttggccgcgg tgtgtggcca tacaacatca cgtggaactg gggcgtggct
     3601 gtctccagat cagagggaga gatcgtgggt gcaaacatgg gagccaaatg gacggataac
     3661 agcggttata cggagaactc tatccttgca ggtggaaaga catacaagct ccctgagaac
     3721 gttttatttg aatatgacag aaaggatttc atgaaaccct ggaagattcg cacagaacag
     3781 tcatccgcga tcgattttga gttcacgcca ttcttcgaca gacatgcgaa atccaatttg
     3841 gtgatcctcg cctcagacgt tcaccagatg atcggaaaat acacaggtga aatcaaaatc
     3901 ggcagaaaaa cagtcgcgat tcatgatgca atcggctggg cggaagatca tatcgccaga
     3961 tggtgacact gtgaaatagc ccgggaagaa tagaaaacca ggcctttggc catattgcgt
     4021 tcgttcgtcg tatgtgttct actatgcgtg ggttgggacc atgaaacgca tccttctact
     4081 ttgcatgctg gcattctcct cgtgtacacg agaggatcca aattacgtat ggctcctcag
     4141 cctcgccgca ggcggtcaaa cgcctccgtc gggagagacg agcgttaccg tagatgtatc
     4201 agatgtcacc ggttcgcctg acttcttgtt cgatacaacg cgaaccattc cagtcttcat
     4261 cacagtgcgg gacccggtgc ctcaagggtc gggcactctg gttcaaatcc tcgacagctc
     4321 aagcgcgcaa gaacttctct ttcaggcaac gtccaacgct cagggaagcg tggaaggaac
     4381 gttcacagta aaccagacca cgaaactggt cactcttcgc gtgaccatcg gaacggacgt
     4441 gcatacctgg accatcaaca tcacagacgt gcaagagatt cgcagattcc tttttgtgac
     4501 tggagaagtg aacatgactc tagtcgcgga tgcggacagt gacggagtcc ctgactctca
     4561 ggatgcctac cctctggatg caaccagatc tgcgatcatt cgtgtccccg cacagggatt
     4621 ctacacagtc gcatttgaag acctgtatcc tgtccagggt gatgcggatt tcaacgacta
     4681 tgtggcagaa gttcgctacg aacaggatct ggacgcaagc ggacgagtcg ctagaatgcg
     4741 gggatttgtc agacacattg cacgaggcgc gggctatcgt catacactgc gcctaacgtt
     4801 accaaccact tcttcttcct tccattctaa actcttcgca gcaaatggaa gcctggaatt
     4861 agaaaacgaa acggccctga ccagctttga aggaatcgag gtaatgccaa acagcggaac
     4921 gaccatcagc tccgcgaact ctgcggcggg cgaagtatat gcgccgggca aatcgagcga
     4981 atttgaagta atcccggcca ctccaatggc tctagcttct cttggaaacg ctccttacga
     5041 tcttcatctt cacgtagtca atacaggata cgatatccat tttctcggaa gatatctgga
     5101 tgcaaacggt cacgatcagt acctggatcc gtctggattt ccatgggcca tcatgattcc
     5161 aggaaactgg agctggccgc tggaaagggt gaacatgcac acggcctatc caaagttcca
     5221 ggaatggtat cagtccgccg gccaggccca aacagactgg tatctcactc ctgcggccgg
     5281 caaggttttc tcgaagtaag cttttgcact gctaggcttt ttgctttcat ctgaatcgcg
     5341 tccggaggag cctggcgcgt gaagttagaa gacagatatc agtctctctt tgaaaaagct
     5401 ccgctgggat tcttcgtagc ggatcgccag ggaaacatcg aggcaaccaa ttcaactctg
     5461 cgaagcctct tttctctcga aaagaagaag aaaccaaacc tgttgacaga aaggcatctg
     5521 gctaacgccg gattcgccac gcagtttcgc ttatgcattt cgcgcgcaga
//
LOCUS       DAORFF010000226         5565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_7993_length_5565_cov_3.936116, whole genome shotgun sequence.
ACCESSION   DAORFF010000226 DAORFF010000000
VERSION     DAORFF010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7993_length_5565_cov_3.936116"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PKK76_10135"
     CDS             <1..303
                     /locus_tag="PKK76_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68875.1"
                     /translation="ELEQIPLRWENLEVIEASSRDRVLVSKEPYMSLGSFVIETPRAG
                     NFVYYVYEEEGRNSIRNLLLEIFKKDYNLEYRAIERVLDLVDSNLGRMVEKRAESD"
     gene            296..1033
                     /locus_tag="PKK76_10140"
     CDS             296..1033
                     /locus_tag="PKK76_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68876.1"
                     /translation="MIDVRSAVIAAYRDHLSRRYTPQNVHRFEILADFPSQRIDEVRD
                     FFLARMYPEADRRLKLDLAFDKLGSVLKSPRKMMPLAGTAISSVFKLGTLIPAALRAG
                     LATFEGYIEIQRLESIMIKEAESRGIEDLSDEKAFAGIIAKIPDRDVQRFRRELTRLF
                     KSLANIPLLETTLEIMGNSIRIISARPDVYTQEELEGIQIGYELLAGGLELFRSLSPE
                     ETQMVLVGVDRIEMDWIERMRAQASVA"
     gene            complement(1048..1827)
                     /locus_tag="PKK76_10145"
     CDS             complement(1048..1827)
                     /locus_tag="PKK76_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNL68877.1"
                     /translation="MSTIALPLPVKRQNSTKTRGKGKKATKSKSRSRQKIVYVEVVRK
                     RSKRWMDEFRSALRAEIANCMTHGMGVGLSLAGMSLLIASAAKAGDPWKIVSFAIYGT
                     CMVLLYLASTLYHGFLSPRAKKVFKVLDHSAIFLAIAGTYTPFTLVSMRGPWGWTLFI
                     IIWALAIGGIAYKMFFVNKYRLISVLIYILMGWLVCIAVKPMFQMIPLAGIIWIAIGG
                     IAYTAGTAFYASSFAMSHMIWHLFVLAGSICHYIAIYFYVL"
     gene            1936..2133
                     /locus_tag="PKK76_10150"
     CDS             1936..2133
                     /locus_tag="PKK76_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68878.1"
                     /translation="MQPNLQDILNAGIGLVLAADEAARNAISSAQKGFEDLKNKGAAD
                     YSDAAIKARSILQDTLRAVRA"
     gene            complement(2184..2507)
                     /locus_tag="PKK76_10155"
     CDS             complement(2184..2507)
                     /locus_tag="PKK76_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002152918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNL68879.1"
                     /translation="MEKQYNTMKEFWPFYLGEHRNKVNRILHFIGSTGVIAFAVCAAY
                     FMDWRYLVAMPLFGYGFAWVGHFFFEKNRPATFKYPFKSLGSDFVMYFYTWTGQIRRE
                     LDKIS"
     gene            2595..3371
                     /gene="proC"
                     /locus_tag="PKK76_10160"
     CDS             2595..3371
                     /gene="proC"
                     /locus_tag="PKK76_10160"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNL68880.1"
                     /translation="MDINLSTRKIGFLGMGKMGSAIARGLKNKHPNLNLTGYDPLIRS
                     DLVNAADSPESLESACDIVLLCVKPQEMKSALASFKGNKQYISIAAGLSTSSIQEMLG
                     GARHIARAMPNLNASIQMAATGIYSHDSELGLLARAIFECVGAVEMVEKEELLHVVTG
                     LSGSGPAFVFEFIDALAQGAVQAGLPYERALRLAAETLIGSASLVVLEKKHPLELRSQ
                     VASPGGTTIAGLAALEELGFRSAVMRAVVRAKERSEELGK"
     gene            3352..>5565
                     /gene="hrpB"
                     /locus_tag="PKK76_10165"
     CDS             3352..>5565
                     /gene="hrpB"
                     /locus_tag="PKK76_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HNL68881.1"
                     /translation="MKSWANSQTRDLPVTGAIPDLKQALASDKRVVLIAPPGAGKTTV
                     VPLELLDEPWLANRSILMLEPRRLAARGAATRMSSMISEKVGGIVGYRVRFENRVSER
                     TRIEVVTEGILSRRIQNDPELEGVGLIIFDEFHERSIHADVGLALSLDVQSSLRDDLR
                     ILVMSATLDGERISGFLSAPLIRAEGRTFPVENRYAGLRKTSPAHAASDAVSALISKS
                     TGDILVFLPGSGDIRRAQELLEEARVDALICPLYGDLRQEEQDRAITPDPSGTRKVIL
                     STNIAETSLTIEGVTTVIDSGLERRLKLDPRTGLGRLETVRISKSSADQRSGRAGRTG
                     PGVCIRLYDEEEYRRMESHAKPEILEADLAPFALEMALWGTADLRFLDPPPASSLSAA
                     RSLLRSLGALDGDTITETGRAMCIPVHPRLARMIVEARSNAQAKIACDLAAILSERDV
                     LRGSGSDLRTRLQALEDARSGSYGPAVHRGSISRALSLATEMRKYAVHSSKDTDAVEP
                     GKLLACAYPDRIARARDEKMERYLLSNGRGAVLPPGDSVRSLFLVAAETRGSGADSSI
                     DLALPVSVESLRELFANQIEIQMETRLDKSGRVQSVRVEKLGALELSVTETSEAVDAG
                     SALLAEVLRQGIDRLSHFQETESLRNRVEFLRRNGEDLPDFSKEYLSANLGEWLGPHI
                     AVMRSISEVQKIDIQPILHGMLSHSQQSILRTHAPESMEVPSGSKISIDYSSPEPA"
BASE COUNT         1348 a         1371 c         1488 g         1358 t
ORIGIN      
        1 gaacttgaac agattccact tcgctgggag aatctggaag tcatagaggc ttcttccaga
       61 gatcgcgtgc ttgtttcaaa agaaccttat atgagtcttg gatcgtttgt gatcgagact
      121 ccgcgcgccg ggaattttgt gtactacgtt tacgaagaag aagggcgtaa ctccattcgt
      181 aacctgcttc tggaaatatt taagaaagat tacaatttgg aatacagggc catcgaacgg
      241 gtgctggatc tggtggattc gaatctgggc cggatggtgg aaaagagggc agaaagtgat
      301 tgatgtgcgc agtgcggtca ttgccgcgta tcgggaccat ttgtcccgcc gttatactcc
      361 tcagaatgtt catcgttttg agattcttgc ggattttcca tcccagagaa ttgatgaggt
      421 tcgagatttt tttctggccc gaatgtatcc agaagcagac cgcagactca agctcgatct
      481 tgcctttgat aagcttggtt ctgtattgaa gtcgcctcgc aaaatgatgc ctcttgcagg
      541 aacggccatc agttctgttt tcaagctggg cactctgatt ccggccgcgc ttcgcgctgg
      601 cctcgctact ttcgaaggct acattgaaat ccagcgactg gaaagcatca tgatcaaaga
      661 agcagaatcg cgcggaatcg aagacctgtc tgatgagaaa gcattcgccg gcatcattgc
      721 aaagattcca gatcgtgacg tgcaaaggtt tcgccgcgaa ctcactcgtc tttttaagtc
      781 tctagccaat attccgcttc tggaaaccac tcttgaaatc atgggcaact ccattcgcat
      841 catcagtgcg agaccggacg tctacactca ggaagagctc gagggcattc agattggata
      901 tgaacttctg gcagggggcc tggaactctt tcgcagcctt tcgcctgagg aaacccagat
      961 ggttctggtg ggtgtggatc gcattgagat ggactggatc gaaagaatgc gcgctcaggc
     1021 ttctgtagcc tgagcaggcc ttattcctta cagcacgtag aagtaaatgg caatgtagtg
     1081 gcagatgctt cctgccagaa caaacaaatg ccagatcatg tgactcatgg caaagctaga
     1141 agcgtagaac gcagtcccag cagtgtaggc aattccacca atcgcgatcc atatgattcc
     1201 ggccagaggg atcatctgaa acattggttt gaccgcgata catacaagcc agcccatcaa
     1261 aatgtagata agtactgaaa tgagtctgta tttatttaca aagaacatct tgtaagcaat
     1321 gccgccaatc gccagtgccc agatgatgat gaacagagtc catccccagg ggccgcgcat
     1381 gctgacgagt gtgaacggag tgtatgtgcc tgcaatcgcc agaaagatcg cagaatgatc
     1441 caggactttg aataccttct ttgcgcgcgg agataaaaag ccgtggtaga gagtggaggc
     1501 aagatacaga agcaccatgc acgttccgta gatcgcaaag cttacgattt tccagggatc
     1561 tcccgctttc gctgcacttg caatgagcag tgacattcca gcgaggctga ggccaacgcc
     1621 catcccgtgg gtcatgcagt ttgcaatctc cgctctcagg gcggatctaa actcatccat
     1681 ccatctcttg cttctttttc tgactacttc cacatagacg atcttctgtc tggagcggga
     1741 cttgctcttt gtggccttct tgcctttgcc tctagtctta gtggaattct gccttttgac
     1801 aggcagagga agtgcgattg tgctcattga accataagaa ccatcctcat gccaggggtt
     1861 gcaaaattgg gttgactatg gtgcggtgca ttaaaaatat ggcgcaacgc actataagtg
     1921 cggaggagta cagagatgca accgaacctt caagatattc tgaatgcagg cattggtctg
     1981 gttctggcag cagatgaagc agccagaaac gcgatatctt cagcacagaa aggatttgag
     2041 gatctcaaga acaagggtgc tgcagactac tcagatgcag ccatcaaagc tcgttccatt
     2101 ctccaggaca ctcttcgcgc cgttcgcgcg taagttcctt gcgcatctgc cggccttgcg
     2161 cttgcgcggg ccggcagcgt agttcaggaa atcttgtcca gttctcggcg gatctgtccg
     2221 gtccaggtgt aaaaatacat cacaaagtca gaaccgagtg atttgaacgg atacttgaac
     2281 gtagcgggtc tgttcttttc gaagaagaag tgtcccaccc acgcaaagcc gtagccgaac
     2341 aaaggcatag caaccagata tctccagtcc atgaagtatg cggcgcaaac tgcaaatgca
     2401 atgacacccg tagatccgat gaagtgaagg attctgttca ccttattgcg atgttcgccc
     2461 aggtagaacg gccagaattc cttcatcgtg ttgtattgtt tttccatgaa tgcctcggtc
     2521 caaacttaca acgacctcct ggatcagtcc attttttttc tgtgcagtgt cgtcgcctgc
     2581 gtcatgcagg ttgcgtggat atcaatctga gcacgcgaaa aattggcttt cttggaatgg
     2641 gcaagatggg gagcgcgatt gcccgcggtc tgaaaaataa gcatccgaac ctgaatctaa
     2701 ccggatatga tcctctcatt cgttcggatc tggtgaacgc tgccgattct ccagaatctc
     2761 tcgagtctgc atgtgacatt gttttgctct gcgtcaagcc tcaggaaatg aagagtgctc
     2821 ttgcgtcgtt taagggaaac aaacaataca tttcgattgc agcgggtctt agcacttcct
     2881 ccattcagga aatgctcgga ggtgcgcgtc atattgcgcg ggccatgcca aatctcaatg
     2941 cgtccatcca gatggcggcc actggaatct actctcacga ttctgaactc gggctcctcg
     3001 ctcgcgccat cttcgagtgc gtaggcgcag tcgagatggt ggagaaagaa gaactgctgc
     3061 acgttgtgac gggtctctca ggatccgggc ctgcttttgt attcgaattc atagatgctc
     3121 tggcgcaggg cgcggtgcag gcaggtcttc cgtatgaacg cgctcttcgt ctggctgcgg
     3181 aaacattgat tggttcggcc tctcttgtgg ttctagaaaa aaaacatccc ctggaactcc
     3241 gttcgcaggt ggcttctcct ggcggaacca ccatcgctgg actggccgct ctagaagaac
     3301 ttggctttcg ctccgccgtg atgcgcgcag ttgtgcgtgc aaaagaaaga agtgaagagc
     3361 tgggcaaata gtcaaacccg ggaccttcct gtaaccggag ccatcccgga tctgaagcaa
     3421 gctcttgcat cagacaaaag agtggttctc atagctccac caggtgcggg gaaaaccacc
     3481 gttgttcccc tcgaactctt agacgaaccc tggcttgcaa atagatccat cttgatgctt
     3541 gagcccaggc gtctggccgc gcgaggagcg gctactcgca tgtcttcgat gatctctgag
     3601 aaggtgggag gaattgtcgg gtatcgcgtt cgttttgaaa acagagtgag tgaaagaacg
     3661 cgcattgaag ttgtcacaga aggaatcctt tcgcgcagaa tccagaatga cccggagcta
     3721 gaaggagtcg ggctcattat tttcgacgaa ttccatgaaa gaagcattca tgcagatgtt
     3781 ggccttgctc tttctcttga tgtacaatct tctttgcgcg atgatcttcg aattctcgtg
     3841 atgtctgcaa ctttagacgg agagaggatt tctggctttc tttctgcacc tctgattcgc
     3901 gcggaaggca ggaccttccc ggtagagaac cgttatgcgg gattgaggaa gacttccccg
     3961 gctcatgctg cctctgacgc tgtgtctgct ctgatttcga aatctacggg agacattctt
     4021 gtgtttcttc cggggagcgg agatatacgc cgcgctcagg agcttctcga agaagcacgc
     4081 gtggatgctt tgatctgtcc gctgtatgga gatctgaggc aggaagaaca ggatcgtgcg
     4141 atcacacctg acccgtccgg tacaagaaaa gtgattcttt ctacaaacat tgcagagact
     4201 tctcttacga tcgaaggagt gaccactgtc atagacagtg gtctggagag aagattgaaa
     4261 ctggatccgc gcaccggtct cggtcgtctg gaaacagttc gcatttccaa atcttccgcg
     4321 gatcaaagaa gcggaagagc cggtagaacg ggccctggag tttgcattcg actctatgac
     4381 gaagaagagt atcgcagaat ggaatctcac gcaaagccag agatactcga agcggacctg
     4441 gcaccgtttg cgctggagat ggcactctgg ggcacggccg atttgcgatt cctcgatcct
     4501 ccgcctgcct cgagtctcag tgcagcgcgc tcgcttcttc gcagtctcgg agccctggac
     4561 ggagatacaa tcacagagac cgggcgcgcg atgtgcattc ctgtgcaccc acgcctggca
     4621 aggatgattg tagaagcacg aagcaatgcc caggcgaaga tcgcctgtga cctggccgcg
     4681 attctttccg agcgggatgt tcttcgcgga tctggatcgg atcttcgcac aaggttgcaa
     4741 gcactggaag atgcgcgaag cggatcgtac gggcctgccg ttcacagggg atccatctcg
     4801 cgagctcttt ctctggccac agagatgaga aagtatgcag tccattcgtc gaaagacact
     4861 gatgcagtgg agcctgggaa gctacttgca tgcgcttatc cggatcgaat cgctcgcgcg
     4921 cgcgatgaaa agatggaacg ttatttgctc tcaaacggaa gaggggcggt gctgccgccc
     4981 ggcgattctg ttcgatctct atttctggtg gcagcagaga ccagaggttc gggtgcggat
     5041 tccagtatcg atctggcact tcctgtttct gttgaatcgc tgcgcgaact ctttgcgaat
     5101 caaattgaaa tccagatgga aacgcggctg gacaaatctg gacgcgtgca gagcgttcgc
     5161 gtagaaaaac tgggtgcact tgaactgtcc gtgacggaga cgagtgaagc agtggatgca
     5221 ggatcggcac ttcttgctga agttttgcgt cagggaatcg atcgcctctc tcactttcag
     5281 gaaacagaat ctcttagaaa cagagtagaa tttctgcgca ggaatggaga ggatcttcca
     5341 gatttctcaa aggagtatct ttccgcgaac ctcggggaat ggttaggccc acacattgcc
     5401 gttatgcgct ctatctcaga ggtacaaaag atcgacattc agccaatact tcacggaatg
     5461 ctttcgcatt cgcagcaaag cattcttcgc actcatgctc cggaaagcat ggaagtgccc
     5521 agcggaagca agatctccat cgactattcg agtccagagc ctgca
//
LOCUS       DAORFF010000227         5557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8015_length_5557_cov_3.340058, whole genome shotgun sequence.
ACCESSION   DAORFF010000227 DAORFF010000000
VERSION     DAORFF010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8015_length_5557_cov_3.340058"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PKK76_10170"
     CDS             complement(<1..388)
                     /locus_tag="PKK76_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68882.1"
                     /translation="MRVLMLILLSWIVSCQSAPIAPGVAKGNGKPSIPAASRAAVFTI
                     LVREVSVSRSEGAPWFSQLTDDDALKVKATLKEDLERVLKFELVPGEKIRAQDLYRAG
                     RFPLHEPYYINPELDPIVRNEAEQELM"
     gene            469..660
                     /gene="thiS"
                     /locus_tag="PKK76_10175"
     CDS             469..660
                     /gene="thiS"
                     /locus_tag="PKK76_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNL68883.1"
                     /translation="MIVNGSEINTGATSLVKLLEDLGIRPDTVAIELNGQIAKRDTWK
                     DVLLKEADKIEIIKFVGGG"
     gene            662..1333
                     /locus_tag="PKK76_10180"
     CDS             662..1333
                     /locus_tag="PKK76_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNL68884.1"
                     /translation="MLPPVYPILDVSTVRAEKLNELELLESWIRSGLNFVQIRWKESG
                     LAEYVSYGQKLKSHFPALRIVANDHYRAVMERPDVFELVHLGQEDYSRIPELPDRVRF
                     GISTHNARQMEVAIAMFKRGLPVSYIALGPVRPTKSKPTGTDPVLSESELAECLQLFR
                     SSSLDLVLIGGIDSENISSIVHPGFARDYGFVPTVAAIRGALGSGLTPIAERIRQERS
                     GLKDR"
     gene            1365..2198
                     /locus_tag="PKK76_10185"
     CDS             1365..2198
                     /locus_tag="PKK76_10185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNL68885.1"
                     /translation="MNGVMEPETYSSAREDRDYLLEYKSVDLRSLRRESVLDVPVDNV
                     TRDEAVALLLDLIEKKKGPHAVMFMDPVKLSAIRPGMKNHWIAEQARVILPDGAGMLW
                     ASRMLRHPLKERIPMIAFLMDIVRLSMKKEFTIYLLGSKVDTVEKAFHNLNKSFPGVR
                     IIGRHGGHFDAERGELVRQALRKSSPDVIFLGMGFPTQEKWYRDNIDFLSRSVVVFVD
                     GAFDVLSGNERKAPDWAQVRGLAWFWRTITRPYRVLRFLRMIRFYVSVVFRNLKMRNS
                     G"
     gene            complement(2201..>5557)
                     /locus_tag="PKK76_10190"
     CDS             complement(2201..>5557)
                     /locus_tag="PKK76_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68886.1"
                     /translation="GPGEEGELDLDAGESGLPDASAFPDGGEASPEEEGAESSFEFEP
                     ESTTSSDSFADSGDLGSDSFSFEPGGEEPSAEVTEPATETPAETFDFGETDLATPEEP
                     ATPATDFDFGSEEEAHADTDFGGGLSTETDPFGAESTESTGDAFAEQTDEPAAEAAES
                     FDFGSPSETPATSDMDFGGETSSFSDFESSPSLGGDDAFGGGEATSSFDLTDSIAPDT
                     DSFGGDSLSSSDTGFDAGGADFSATSDFGSSDFSAGESAGSTPAPDAGVASFDATADE
                     SDLGDLTTDLTGLSDLAPSDSDSDSGAFDTGDLSDMTRQANLSQGIGDEFTDEELAKI
                     RARLDEIPPGLKKAIITATVDEKVSRSDQQLIMHMLIDQATPDAIADFLEPRIGYRPD
                     LTPPQYRKDGVQIIYADGMSPEEIAGRRKKVRITIFAVLGGVAATLIAFGSVLLYNKL
                     SIAGRYKDGLAELSQAALTTGNEREEHRKRAEAFYQKALSSSGTYDPEVMNKYGLAYL
                     RAGYYDDAFEKLFGRVEPAYDWADPAARAPLIRLKDGSKFPSPEQQAKGDRAEFLDRQ
                     NVKRKLEVPGAYTVTRLRDGNLDKRTLLSLGKFHSHNAQSFTRRPEGQKYKNDDLGID
                     YYRLILTLMEMPDDVDARSGIGEVYYNRKEYASAAREYNKIIEKFPNEIKGHAGLLNT
                     YIQIWHENQDPRYVLEKHRLIQRLGLEKDLPIYLLTKLAGFYIDLNEDDVRIRYQVDP
                     VNSVTGLGLEDNATHLLELVFKKEDERDGEIINGASYGEGFYQRGRFLLKQKETLRAL
                     RQFQNAHQHDPRHYLAVREMGAYYRDNLDFARAEEYFKKSLELYNQYKDDYGAKPEDE
                     TLIAGDRGLLFYDMGSLLFLKYAGTPRENKEAFADSRIYPDRGRNAETSETQKRRELL
                     SQARDYFGQALKEEIRDEKAKVQSIYWMGWIDYISSDFESALVEWEGLQDIYSGNYSD
                     PVLLMARANSYYYTDQNRSALGDYLKLQNDLERRALSIDHAVPEDPEHQKVFLTLAAL
                     YNNLGAVYEKEFNEAKKRGASQRSLEDLEKNSLKYYWKSIESARKVEEDNEIARTNLQ
                     KAFRSAVKSDPALDDWISPALPGLTEKKKK"
BASE COUNT         1239 a         1367 c         1453 g         1498 t
ORIGIN      
        1 tcatgagttc ttgctctgct tcgtttcgca caatcgggtc gagctcgggg ttgatgtagt
       61 atggttcgtg aagtggaaat cttcctgccc tgtagaggtc ctgtgcgcga attttctctc
      121 cgggcaccag ttcaaacttg agtacccgtt ccagatcttc ttttaacgtt gctttcactt
      181 tcagagcgtc atcatccgtg agctgggaaa accatggcgc cccttctgat ctgcttacgg
      241 acacttcgcg caccagaatg gtgaatacgg ccgcgcgtga tgcggctgga atggaaggct
      301 tcccatttcc tttcgcaact ccaggtgcga tcggtgcgct ctggcacgaa acaatccagg
      361 aaagcaaaat caacatgagt acgcgcatag gttgaacctg aaaaaaccgg cgcgcccgtc
      421 gacgacttct gttgcttgac agcctctgtt tttatttttt gtggaggtat gattgtaaat
      481 ggcagcgaga taaacacggg tgccaccagt ctggtgaagc ttctagagga tctgggcatt
      541 cggccggata cagttgccat tgaactgaac ggacagatcg cgaagagaga cacctggaaa
      601 gatgtgcttc taaaagaggc agataaaatc gaaatcatca agttcgttgg aggaggatga
      661 agtgcttcct ccggtctatc cgatcctcga tgtaagcaca gttcgcgcag agaaactgaa
      721 cgaactcgaa ttgctcgaaa gctggattcg cagcggcctg aatttcgtgc agatcagatg
      781 gaaggagtcg ggcctcgcag agtatgtatc ctacggacaa aaactcaaat cacattttcc
      841 tgctctgcgc attgtagcca acgatcacta ccgcgcagtg atggaaagac cggatgtttt
      901 tgagctggtt catctgggtc aggaagacta ctcgcgaatc ccagaattgc ctgaccgggt
      961 ccgttttgga atctccacac acaatgcaag gcagatggaa gtggcaatcg ccatgtttaa
     1021 gagaggcttg cctgtatcct acattgcact cgggcccgtt cgtccaacaa agtccaaacc
     1081 taccggaacg gatcctgtcc tgagtgaatc tgaactggcg gaatgcctgc agctgtttcg
     1141 atcgtcctct cttgatctgg ttctgatcgg tggaatcgac tcggagaaca tttcttctat
     1201 tgttcaccct ggttttgcaa gagactacgg ttttgttccc acagtagcag ccatccgggg
     1261 tgccctgggt tctggcctga ctcccatagc agaaaggatc cgccaggaga gatcggggct
     1321 gaaagatcgt tgactggatg ggtgcgcgac ataatctgat tcccatgaac ggcgtgatgg
     1381 agccagaaac atattcgtct gcccgggaag accgggacta ccttctggaa tacaaatcgg
     1441 tcgatctgcg cagtctcaga cgcgaaagcg ttctggacgt tccagtagat aacgtcactc
     1501 gcgacgaagc agtggcactt ctcctggacc tcattgaaaa aaagaaaggt cctcacgcag
     1561 tcatgttcat ggatccagtg aaactttctg cgatccgtcc tggaatgaag aatcattgga
     1621 tcgcagagca ggccagagtg atcctgccgg acggcgctgg catgctctgg gcttcgagga
     1681 tgcttcgtca tcctctcaaa gaaagaattc caatgatcgc gtttctcatg gatatcgttc
     1741 gtctttccat gaagaaggaa ttcacgatct atttgcttgg ttccaaagta gatacagttg
     1801 aaaaagcatt tcacaatttg aacaagagct ttcctggagt tcgaatcatc ggacgccacg
     1861 gtgggcattt tgacgcggag cgtggagagc tggtgcgcca ggctctgcgt aagtcgtcgc
     1921 ctgacgttat ttttcttggg atgggttttc ccactcaaga gaaatggtat cgagacaaca
     1981 ttgacttctt aagccgttct gtagttgtct tcgtggatgg agcttttgat gttctttcgg
     2041 gtaacgagcg taaggcgccg gattgggcgc aggtgcgcgg gcttgcctgg ttctggagaa
     2101 ccatcaccag gccatacaga gtccttcgct tcctgaggat gattcgtttc tacgtttccg
     2161 tggtcttcag aaatttgaag atgcgaaaca gcggctgatc ttatttcttt ttcttctcag
     2221 taagacctgg gagggcaggc gaaatccagt cgtcgagagc cgggtctgat ttcacagcag
     2281 acctgaaagc cttctgcaaa ttcgtgcgcg caatttcatt gtcttcttct actttgcgcg
     2341 ccgattcaat cgacttccag taatacttca gagaattctt ctccagatct tccaggcttc
     2401 tctgagatgc gccgcgtttt ttggcttcat tgaattcctt ttcataaaca gcacccagat
     2461 tgttgtacag ggccgcgaga gtcaggaata ctttctggtg ctctggatct tctggcaccg
     2521 cgtgatcaat ggatagagcg cgtctttcca gatcgttttg cagtttcaga tagtctccga
     2581 gagcagaacg attttgatcc gtgtagtagt aagaatttgc gcgagccatg agcaatacag
     2641 ggtcagagta gttgccagaa tatatatcct ggaggccttc ccattcaacg agcgcgcttt
     2701 caaagtcaga ggaaatgtag tcaatccagc ccatccagta aatggactgc acttttgcct
     2761 tttcgtcgcg gatctcttct ttgagagctt gaccgaagta gtcgcgagcc tgagacaaca
     2821 gctctcgcct cttctgggtt tctgatgttt ccgcgttgcg cccgcgatca ggatagatgc
     2881 gcgaatctgc gaatgcttcc ttgttttctc tgggcgttcc cgcatatttc aggaagagga
     2941 gggagcccat gtcgtagaac agtagcccac ggtctccggc gatgagcgtt tcgtcttccg
     3001 gttttgcgcc gtagtcatct ttgtattgat tgtagagctc cagagatttc ttgaaatatt
     3061 cctctgctct tgcgaaatcc aggttatctc tatagtatgc gcccatttcg cgcacggcaa
     3121 gatagtggcg tgggtcgtgc tgatgcgcat tctggaactg acgcagagcg cgaagagttt
     3181 ctttctgttt caggaggaag cggccgcgtt gatagaatcc ttcgccgtag ctggctccgt
     3241 tgatgatttc tccgtctctt tcgtcttctt tcttgaaaac aagttcgagt agatgagttg
     3301 cattgtcttc tagacccagt cccgtcacag agttcactgg atctacctga taacgaatgc
     3361 gaacgtcatc ttcattcaga tcaatgtaga atcccgcaag ctttgtgagc aggtagatgg
     3421 gtagatcttt ttcaagtccc agcctttgaa tgagacgatg tttttcgagt acgtatcgcg
     3481 gatcctggtt ttcgtgccag atctggatgt aagtgttgag caggcccgcg tgtcctttga
     3541 tttcgtttgg aaacttctct atgatcttgt tgtattcacg cgcagcagat gcgtactctt
     3601 ttctattgta atagacttca ccaatgcctg agcgtgcatc cacgtcatcc ggcatttcca
     3661 tgagtgtgag aatcaaacga tagtagtcga ttcccaggtc gtcgttcttg tacttctgtc
     3721 cttctggtct tcgagtaaag ctctgcgcat tgtgtgaatg aaacttacca agagaaagta
     3781 atgtgcgctt gtcgaggttg ccgtctctca gacgagtgac tgtgtaggca ccgggaactt
     3841 ccagtttgcg ttttacgttc tgtctatcga ggaattcggc tctgtctccc tttgcctgtt
     3901 gctcggggga gggaaacttg cttccatctt tgagtcggat gagaggcgcg cgtgcagctg
     3961 gatcggccca gtcgtaggct ggctccacgc gtccaaatag tttttcgaaa gcatcatcgt
     4021 agtatccggc acgcagatag gccaggccgt acttgttcat tacttccgga tcataggttc
     4081 cgctcgaaga aagtgctttc tggtagaatg cttccgcgcg ctttctgtgc tcttctcttt
     4141 cattccctgt agtcagcgct gcctgggaga gttctgccag gccgtctttg tatctgcccg
     4201 cgatggaaag cttgttgtag agcagaacag aaccaaaggc aatcaacgtg gcagcaactc
     4261 cacctaacac tgcgaagatg gtaatcctga ctttctttct gcgcccggcg atttcttccg
     4321 gactcatgcc atccgcgtag atgatctgta cgccgtcctt tctgtactgg ggcggagtca
     4381 gatccggacg atatccgatt cttggttcga ggaaatctgc gattgcatca ggcgttgcct
     4441 gatcgatcag catatgcatg atgagttgct gatcgcttct cgatactttt tcgtctacag
     4501 tggcggtgat gatcgctttt ttgagtccag gcggaatctc atccaggcgt gcccgaattt
     4561 ttgcgagttc ttcatccgtg aattcgtctc cgattccctg cgacaggttt gcctgacgcg
     4621 tcatgtcgga gagatcacct gtatcaaatg cgcctgaatc cgaatccgaa tcagaaggag
     4681 ccagatcaga tagacctgtc agatcggtgg taagatctcc tagatccgac tcgtctgcag
     4741 tggcatcaaa agacgcaact cctgcatctg gagcgggtgt tgagcccgca gattctcctg
     4801 ctgaaaaatc ggaactgcca aaatcagaag ttgcggaaaa gtcagcgccg ccggcgtcaa
     4861 agcctgtatc agaagaagaa agagagtcgc cgccgaatga atctgtgtcc ggagcgatcg
     4921 aatcggtcag gtcaaacgaa gaagtagctt ctccgccgcc aaacgcatca tcgccaccga
     4981 gggaagggga ggattcaaaa tcagagaaag aagaagtctc cccgccaaag tccatgtcag
     5041 atgtggccgg tgtttcggaa ggagagccaa aatcaaaaga ttcggccgcc tctgctgctg
     5101 gctcatccgt ttgttccgcg aacgcatcgc cagtagattc tgtggattct gccccgaatg
     5161 gatccgtttc agtggaaaga ccgccgccaa aatctgtgtc tgcatgggct tcttcttctg
     5221 atccaaaatc aaagtcagtc gctggagtgg ctggttcttc cggagtcgcc agatctgttt
     5281 cgccaaaatc gaatgtctct gcaggtgttt cagtggcggg ttctgtgact tctgcagatg
     5341 gttcttctcc gcctggttca aatgaaaacg agtcggatcc gagatcgcct gaatctgcaa
     5401 atgaatctga agaggtcgtg gactctggtt caaattcaaa gctggattct gcgccttctt
     5461 cttcggggct tgcttcgcct ccatcaggaa atgcagatgc gtccggcagg ccggattcgc
     5521 ctgcgtctag atccaattcc ccttcttctc cgggtcc
//
LOCUS       DAORFF010000228         5539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8059_length_5539_cov_3.937819, whole genome shotgun sequence.
ACCESSION   DAORFF010000228 DAORFF010000000
VERSION     DAORFF010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8059_length_5539_cov_3.937819"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKK76_10195"
     CDS             <1..245
                     /locus_tag="PKK76_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68887.1"
                     /translation="NKILESHEQWADRSQILSYIAVIPAALALFIARFEKHMKVIAFA
                     VLLAAGACIVVAGHEGAKLVHIHGIGAEGNYIKDSK"
     gene            270..530
                     /locus_tag="PKK76_10200"
     CDS             270..530
                     /locus_tag="PKK76_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68888.1"
                     /translation="MKHKVLMAAAIAGLALGAVAAHEGEDHSKDKGKCFGINSCTGKG
                     QCATKEHTCAGMNTCKGKGWIKLTKAECEKKGGKFDASKPDM"
     gene            644..2200
                     /locus_tag="PKK76_10205"
     CDS             644..2200
                     /locus_tag="PKK76_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68889.1"
                     /translation="MKMSPGAFAFIFSIFSAAGLGAQAFEGRLAVGSFHNCAIVENGK
                     VVCWGLGDTGQIGDGKLVNALTPRFVSGITNAKSLAAGEHWTCALLADSSVKCWGFIA
                     APWAPASEKSKTRPCAWGSHTDPCTPVPVSVPPAQGITSIMGMATMTYGILPDGKIVV
                     WTGNDGTANLLKDGKNIQLSGRSSMVIDWIPDAKEVATIYVSTGDMPSHMCAVTKKGR
                     IYCHGATPAVYGPRKPICTAEPRIKPVCQIDFQGEKISVTGEDAPRMCKYAASLDPQM
                     MKSYEWYCQGQTCTYREVPLQKATHLWYLIMANGKEIKSHVTGYAEIFKSTEAEKSKC
                     RDDFYTVELDATPMEGLTDVRRVSLYSDSSAFGITNKDEVYYWGSGREDRDILPDVVP
                     ESSGKPIPPVKIPQFTGAISIRHMSWNGCALFKTGIVKCWGVAGDEGRLGNGIFGDEN
                     NETTRTPVQVKGIRDAVYHGMGHSHSCVAVKDGRVFCWGLNDSGQLGNGQTKNSAEPV
                     EVKGVVLNFQ"
     gene            2247..3722
                     /locus_tag="PKK76_10210"
     CDS             2247..3722
                     /locus_tag="PKK76_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNL68890.1"
                     /translation="MFKLHWLDLTLIGAYLALMVYMGKVLSTKIKSESDYYLAGKSLP
                     WYIIGLSIIGTNIGSNDYLGASGGAYRIGIAQANFEWIGAIPAMIISAFIFIPFYWRA
                     GVYSIPEYLGRRYNQPVRVISATILSLFAILIVGVYHWASALMLETYMGWPMYVSIPI
                     TAAVVGFYTISGGLRADTFTDAIQVTLMFVSSLFLAYFGVQKAGGPEAFLHTLSTKFP
                     GHMHAFLPSDHPDFPWPGVILGLGFVLSPAYWCANQAILLRTLGARSEWDGRASMIFA
                     ALAKTFVPVLIILPGFVALALSTEKLAVSDSALPWVVKNVLPPGPSGLFFVAFIAALQ
                     ASVSSALNSTAVMVTRDILGVLRPKPRTDAQVVRLGRICTFLVLALGVASAPLTAKFE
                     GIYIFVQTALSLFQGPMFALIVVGILYKYATPAAAVVSLVLGLLLAAILQIASFNMLY
                     IAFWTFVFSGFLLFVVSMFTARKPDEELVNLCYTTTVKKYV"
     gene            3715..3915
                     /locus_tag="PKK76_10215"
     CDS             3715..3915
                     /locus_tag="PKK76_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68891.1"
                     /translation="MFNWIHTLPLWTANALAMGLFGLILVICWSLPSAFVWSGSKRVF
                     YKDLRIWATLLTLIQIIIYCFV"
     gene            3940..>5539
                     /locus_tag="PKK76_10220"
     CDS             3940..>5539
                     /locus_tag="PKK76_10220"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNL68892.1"
                     /translation="MTELLRTGAAYYPDYIPEFVRTWRNGNVETISRRERMIQDFARM
                     KRNGFTWIRMGEFSWSLVEPEPGAFNPALFLEALDLALSHEIDVIFCTPTATPPPWLT
                     ERNPEILPRDKSGRLMPAGTRRHYDPTNAAYRLESRRITEYYARTFGNHPAVKCWQTD
                     NEFGCHGSTYVYTESAASWFRAWLKDNYLDIHSLNEAWQTSFWSQRYTDFSQIPLPLS
                     SYADANPGLELDFRRAMNHAWKIFQSEQAILLRELSPGRPIIHNFMTLFFELDPWKMS
                     EDLDLAGFDHYQMESDPHPISSAWQFRLMASLKKRSFMVLEQQPVQVNWQKVNRRFPM
                     NLLFQWGMQAMFAGADSMLYFSWQRMYGGAEQYHDGIVSHDIRVDTTEQEELLQFQRS
                     FLSALKTAGIQKIDPLGDVCMVLDFESIWSHDITSQSELFSTRTVVDVAAEFCLSQGL
                     GFSFSPSIESIDPSSRMVFLPAHSFELSEKERSVLRQYMEEGGIVCSFPRSLMKRRDN
                     RMSDLPLHLLHPEDLRVLSYGALLE"
BASE COUNT         1328 a         1433 c         1394 g         1384 t
ORIGIN      
        1 ccaataagat cctcgaaagc cacgagcagt gggcggatcg ctctcaaatc ctttcttaca
       61 ttgcagtcat ccccgccgca ctcgccctgt tcatcgcacg ttttgaaaag catatgaagg
      121 ttatcgcgtt cgcagtccta ctggcagcgg gtgcgtgcat tgtagtggcc ggtcacgaag
      181 gagccaaact ggttcacatc catgggatcg gtgcagaagg caattatatc aaggatagta
      241 agtaaaccaa atcgttcagg agaacagttg tgaaacataa agtattgatg gccgcggcca
      301 ttgcaggact cgcactcggc gcggtagccg cacatgaagg agaagatcat tccaaagaca
      361 aaggaaaatg ctttggcatc aactcctgca caggcaaagg tcaatgcgcc acaaaggaac
      421 acacttgcgc cggaatgaat acgtgcaagg gaaagggctg gatcaaactc acaaaagcgg
      481 agtgcgaaaa gaaaggcggc aaattcgacg cgtccaaacc tgacatgtaa tcaaaaagaa
      541 ctcacctgac caggtttgac cctgtcggcg gcatgcctgg tcgcaattcg catcgccgga
      601 aaacaacttg atccggggaa ccattcgctt ctgagttggc ctcatgaaaa tgagcccggg
      661 agcctttgca tttatcttct ccattttttc tgcggcgggg cttggtgctc aggcctttga
      721 agggagactt gcggtaggca gctttcacaa ctgcgccatc gttgaaaatg gcaaagtggt
      781 ttgctggggg ctcggggaca caggtcaaat cggagatggt aaactggtca acgcactgac
      841 tcctcgattc gtgagtggaa ttacaaatgc aaagagtctc gcagcaggcg aacactggac
      901 gtgcgccctt cttgcggatt catccgtcaa gtgctgggga ttcatcgcag cgccctgggc
      961 gccagcatcc gagaaatcaa agacaaggcc atgcgcatgg ggcagccata cagatccatg
     1021 tacgcctgtt cccgtttctg ttcctccagc tcaaggaatt acatcgatta tgggaatggc
     1081 cacgatgacg tacgggattc tacctgacgg aaagatcgta gtgtggaccg gcaacgatgg
     1141 aacagccaat ctgctcaaag acggaaaaaa tattcaattg agcgggcgca gttccatggt
     1201 cattgactgg attcccgatg caaaagaagt agccacgatc tacgtgagca caggcgatat
     1261 gccttcgcat atgtgtgccg taacaaagaa aggccggatc tactgccatg gcgcgacacc
     1321 ggctgtgtat ggcccgcgaa aaccaatctg cacggcagaa cccaggatta aaccagtatg
     1381 ccagatagac tttcagggag agaagatttc agtcacaggc gaagacgcac cgcgcatgtg
     1441 caagtacgct gcatctctcg atccgcaaat gatgaaatcg tacgaatggt attgccaggg
     1501 acaaacctgc acttaccgcg aggtccctct gcagaaagca acgcatcttt ggtatctcat
     1561 catggcaaac ggtaaagaaa tcaaatcaca tgtaacaggg tatgcggaga ttttcaaatc
     1621 cacggaagca gaaaaatcca aatgcaggga tgatttttac acagtggagc tggatgcaac
     1681 gcccatggaa ggactaaccg atgttaggcg agtatccctc tactctgaca gcagcgcgtt
     1741 tggaatcaca aacaaagacg aagtctacta ctggggtagc ggtcgcgaag acagagatat
     1801 actgccggac gtggtgccag aatccagcgg taaacccatt cctcctgtca agattccaca
     1861 atttacggga gctatatcca ttcggcacat gtcctggaac ggatgtgccc tattcaaaac
     1921 cggaatcgtg aaatgctggg gagtcgcggg agatgaagga cgtctcggaa acggaatttt
     1981 cggggatgaa aacaacgaaa ccaccagaac gccggtccag gtaaagggca tcagggatgc
     2041 tgtgtatcac ggaatggggc acagccattc ctgcgtcgcc gttaaagatg gccgcgtttt
     2101 ctgctgggga ttgaacgatt ctggccagct cggcaacggc cagacaaaga actcagcgga
     2161 accggtggaa gtcaaaggtg ttgtcctgaa ttttcagtag cggtaatcgc gcctaatgaa
     2221 tgttaggcga tcaaggggca acgcatatgt ttaaacttca ctggctggat ctgacgctga
     2281 tcggggcgta cctcgcactc atggtctaca tgggcaaggt cctatccacg aaaatcaaat
     2341 cagaaagcga ctactatctg gcgggtaagt ctttgccctg gtacatcatc gggctttcca
     2401 tcatagggac aaatatagga tcaaacgact atctgggtgc gagtggcgga gcgtatcgca
     2461 tcggaattgc acaggcaaac ttcgagtgga tcggcgcgat tcccgcgatg atcatctccg
     2521 cattcatatt cattcccttc tactggcgcg caggtgttta ttccatcccg gagtatctgg
     2581 gtcgtcgcta caaccagccg gtgcgggtga tttccgccac aatcttgtct ctattcgcga
     2641 tcttaattgt gggcgtctat cactgggctt ccgcattgat gctcgaaaca tatatgggct
     2701 ggcctatgta tgtgagtatt ccaatcacgg cggctgtggt aggattctat actatttctg
     2761 gaggtcttcg cgcagatacg ttcacagatg caatccaggt aacgcttatg ttcgtcagct
     2821 ctctgtttct tgcttacttc ggcgtccaga aggcaggcgg gccggaagcg tttcttcata
     2881 ctctctctac aaaattccca ggacacatgc atgcatttct tccatcggat catcctgatt
     2941 ttccgtggcc tggagtgatc cttggacttg gctttgtact ctctccggca tactggtgcg
     3001 ccaatcaggc gattcttctt cgcacactgg gtgcgcgctc agagtgggat ggtcgcgcat
     3061 ctatgatctt tgctgccctt gccaaaacat ttgtgcctgt gctcatcatt ctaccaggat
     3121 tcgtcgctct ggctctctcc acggagaaac tcgctgtgag tgattccgct ctgccatggg
     3181 ttgtaaaaaa cgttctccca cccgggccat ccggtctatt cttcgtggca ttcatcgcag
     3241 cactccaggc ttccgtgagt tccgcgctca actctactgc agtcatggtc actcgcgaca
     3301 ttcttggtgt gcttcgtcct aaacctcgca cagatgctca ggtggtacgc ctgggtcgaa
     3361 tttgcacgtt tctggttctt gcgctcggtg tggcatctgc accgctgact gcgaagtttg
     3421 agggcatcta catctttgtg cagacagcac tctctctttt ccagggtcct atgttcgctc
     3481 tcattgtagt tggaattctt tataaatatg ccacacccgc ggcggccgtg gtgtctctgg
     3541 ttcttggact tctgctcgca gccatcttac agattgcatc cttcaacatg ctctacattg
     3601 ctttctggac ttttgtattc tctggcttcc tgctttttgt tgtaagtatg ttcactgcgc
     3661 gaaagccaga tgaagaactt gtgaatcttt gttacactac cacggtgaaa aaatatgttt
     3721 aactggatcc atactctccc tctctggact gcgaacgctc tggccatggg tttgttcgga
     3781 ttgattctgg tgatttgctg gtccctgccc tccgcgttcg tctggagtgg ttcgaaaaga
     3841 gtattctaca aagacttacg aatctgggcc actcttctga ctctcatcca gatcatcatc
     3901 tactgcttcg tgtaaggtgc gttccatgca gactgcgtta tgacagaact acttcgcacc
     3961 ggtgcggcgt actatccgga ctatattcca gaatttgtgc gaacgtggcg caatggcaat
     4021 gtggaaacga tttcgcgtcg cgaacgaatg atccaggatt tcgcgcgcat gaagcgcaat
     4081 ggctttacgt ggattcgcat gggagaattc agctggagcc tcgtggaacc cgaaccagga
     4141 gcatttaacc cggctctttt tctagaagcc ctggatcttg ccctttctca cgagatcgat
     4201 gtaattttct gcactcccac cgcaactcca cctccctggc tcacagaacg aaatccagaa
     4261 attctgccgc gagataagtc cggtcggctc atgcccgcgg gcacacgaag gcattacgat
     4321 ccaaccaatg ccgcctatcg tttagaaagt cgtcgcatca ccgaatacta tgcgcgaaca
     4381 tttggaaatc atcctgcagt caaatgctgg cagacggaca atgaatttgg atgtcacggc
     4441 tctacttacg tttacactga atcggcagcc agctggtttc gggcatggct caaagacaac
     4501 tacctggaca tccattctct caatgaggcc tggcaaacat ctttctggtc acagcgctac
     4561 accgatttct cgcagatccc gcttcctctc tcatcttacg cggatgcgaa tcccggcctc
     4621 gaacttgatt ttcgccgtgc gatgaatcat gcgtggaaga tctttcaatc ggaacaggcc
     4681 atccttcttc gcgagctttc gccaggtcgt ccgatcattc acaacttcat gactctcttc
     4741 tttgaactgg atccgtggaa aatgtcggaa gatctggatc ttgctggatt tgatcactat
     4801 caaatggaat cagatccgca cccgatcagc tccgcatggc aatttcgtct gatggcttct
     4861 cttaagaaga gatccttcat ggttcttgag caacaacccg tacaggtaaa ctggcagaaa
     4921 gtcaatcgtc gctttcctat gaacctgctc ttccagtggg gaatgcaggc catgttcgca
     4981 ggcgcggact ccatgctcta tttttcctgg caaagaatgt acggtggtgc cgagcagtac
     5041 cacgacggaa ttgtatcgca cgacattcgc gtagacacaa cagagcaaga agagcttctt
     5101 caattccaaa gaagctttct ttccgccctc aaaacggccg gcattcagaa gatcgatccg
     5161 ttaggcgacg tttgcatggt tcttgatttt gaatccatct ggagccatga catcacctcg
     5221 cagtctgaac tgttttctac aaggaccgtg gtcgatgtgg ccgcggaatt ctgcctttcg
     5281 cagggtcttg gtttttcatt ttctccttcc attgaatcca tcgatccttc ttccaggatg
     5341 gtctttctac ccgcgcattc gtttgaactc agcgaaaagg aacgctccgt tctgagacaa
     5401 tacatggagg aaggcggaat cgtttgctcg tttcctcgaa gcctcatgaa acggcgcgac
     5461 aatcgcatga gcgatttgcc gctgcatttg cttcatccgg aagacttgcg agtcctatct
     5521 tatggagcgc tcctcgagg
//
LOCUS       DAORFF010000229         5507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8118_length_5507_cov_3.274395, whole genome shotgun sequence.
ACCESSION   DAORFF010000229 DAORFF010000000
VERSION     DAORFF010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8118_length_5507_cov_3.274395"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PKK76_10225"
     CDS             <1..873
                     /locus_tag="PKK76_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004428888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNL68893.1"
                     /translation="LGAMALGAQRILPVMQATFVSWTNVKISQSTMREVLGFLDQPLP
                     AYATEPPPIPMAFKDRITLDHIGFRYSEEGPWVLRDVTMEIPHGSRVGIVGITGSGKS
                     TLLDILLALLVPTEGRLLVDGVTIDSTTMRAWQANVAHVPQMIYLSDTSIAENIAFGV
                     PADQIDMDRVKRAASQAQISSHIETLQAGYQTFVGERGIRLSGGQRQRIGIARALYKQ
                     ASVIVFDEATSALDNETEQAVMEAIEGLGKDLTILMIAHRLSTVKNCDQVIELGAGQI
                     RRAGKYEELFAAHN"
     gene            888..1937
                     /locus_tag="PKK76_10230"
     CDS             888..1937
                     /locus_tag="PKK76_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002178886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNL68894.1"
                     /translation="MQIKLFDKIVGDGQPVFVIAEVGLNHNGSMEIAKQLIDAAKAAG
                     CDAVKFQKRHVPSLAVRSFLDAPDLRFPAFGSTYREVRQAVEFSTSQLMELRDYSRSK
                     AIPFFVTPFELNSAKEIDALEPEVIKSASHNLTFIPLLEVLAQKRKPLIVSTGMSTIE
                     ELDMAVSVIRKYDCPFALLHCVSAYPTPFSLVQLRMIDLLRERYGVPVGYSGHEEFSA
                     KSLPTLAAVARGACIVERHITLDNNMPGFDHKISLNPADLKEVVLQIRAIESMMGDGK
                     KHLLPEELVKREQQRVSIATARAIRAGEIISSEMISFRGPGTGFATYEINNVVGKKAV
                     VDISEDVLIEKAMLS"
     gene            1941..3200
                     /locus_tag="PKK76_10235"
     CDS             1941..3200
                     /locus_tag="PKK76_10235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNL68895.1"
                     /translation="MPRVSIIIRAKNEEYWLPRLLPQLKRQSYQDFEVILVDNQSSDA
                     TCAIFRKEYPSGRILEIGDFKPGRAINAGIEAASGELVVIISAHCVPAEESWLASFVS
                     AMDDTTIGAAYGRQLPMHTSHPLDKRDLLNTFGIEFRVQKKDTFFHNANSILRRSVWE
                     RFPFDEETPHIEDRLWAAQLIENQIWIAYTPGAVVYHHHGINHHANIKRAEAISQILG
                     SGRLGYSMELPEFMRAENSHTLYCFLGHDAGTHTVLLSLLGQMKDAPPGRFIVHSAQA
                     ELLRDLPVGPDVLPYAADDRERTFVEILAELIEDEAARNFFPDAVVYINLRSPGLKWE
                     LLREAIHCYYGDMYESVFWANREYSNIWQKDNGVYAQLRADYTRREKKEPVYIARYGL
                     GTVTAPALIRSGALIGNKVAILPLEDN"
     gene            3252..3971
                     /locus_tag="PKK76_10240"
     CDS             3252..3971
                     /locus_tag="PKK76_10240"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNL68896.1"
                     /translation="MTTKPRILGITLARGGSKSVPHKNIRPILGVPLIAYTIRAALAS
                     QYLTRYIISTDDPEIRDTAIRYGADAPFLRPAEFSTDTASSVSALQHAVSWVEKEENS
                     KYDYIIEMMCTNPLKTTGDIDSCIQKLVETGADSVIAVHKLDDHHPIRIKKIVDDLIV
                     DFCLPEVPEMRRQDLKPDAYIRSGSIYALSRKHLMEENRRFGSKHSRPYILPPERAIN
                     VDTEIDFLIAEKILEKAASGT"
     gene            3976..4941
                     /locus_tag="PKK76_10245"
     CDS             3976..4941
                     /locus_tag="PKK76_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNL68897.1"
                     /translation="MPRILVITPVLHIAGVPEVLESAGEVTYLDDPEPSEVIGQISSY
                     DAIFTNPNKSRVYIGEEILSAGNLKAICTASTGTNHIDKKAAAARNITVLSLTEQREV
                     INRISSTAELAFALTMAQLRKVLWSHAGALEGEWDYTHYIGRQLNSLTVGVIGYGRLG
                     SLYAGYCRAFGAQVLVYDPYKKVDRADISQLATIEELFERSDVISIHVHVSPETTNMI
                     HAASFARMKKDVLLINTARGEIINESDLVGFLKNNPEARAGVDVLADEIKNRLNSPLL
                     DYAKSGRQVVITQHIGGMTREAQEIAYGHAARMLHSFLRGSANPV"
     gene            4952..>5507
                     /locus_tag="PKK76_10250"
     CDS             4952..>5507
                     /locus_tag="PKK76_10250"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNL68898.1"
                     /translation="MKYISEIDSIRAFACLMVMWDHLYSRFFLRIGDRELFTGGFIGV
                     DVFFVLSGFLISSLLFQEKERSGRIRYGKFYVRRALRLLPPIFTVVPLVLLPLIYYFD
                     GAAVTILNYFYLVTYTTVLPKFLEVTHRFPNPYWFPHAWSLSIEEAFYLVFPFLVGQI
                     QNLKRFSWSLLFFFFLNACMMPYVL"
BASE COUNT         1301 a         1356 c         1404 g         1446 t
ORIGIN      
        1 cttggtgcga tggctctcgg ggcccagagg attctgcctg tgatgcaggc aactttcgtc
       61 tcctggacca acgtgaaaat tagccagtct actatgcggg aagtgcttgg cttcctcgat
      121 cagcctctcc cggcctatgc tactgagcca ccaccgatac caatggcctt taaggatcgc
      181 ataacgttag atcatattgg ttttcgctat tccgaagaag gtccgtgggt tcttcgcgac
      241 gttacgatgg aaatccctca tggatctcga gtgggaattg ttggcatcac tggaagcgga
      301 aaaagtacac tgctggatat tcttctggca ctccttgtcc ccacggaagg tcgacttctt
      361 gtcgatggcg taaccattga tagcactacg atgcgtgcgt ggcaggcgaa cgtggcccat
      421 gtgcctcaga tgatttatct ttctgatacg tcgatcgcgg agaatatcgc ttttggcgtg
      481 ccggcagatc agatcgacat ggatcgcgtg aagcgcgcag cgtcgcaggc ccagatcagc
      541 tctcacattg aaacactgca ggccggatac cagacttttg tcggagaacg cggcatcagg
      601 ctctcaggtg gacaacgcca gaggatcggc attgcaagag cactctacaa gcaagcatcc
      661 gttatcgtct ttgacgaggc gaccagcgcg cttgataatg aaactgagca agcggtgatg
      721 gaagcaatcg aaggtcttgg taaagatctg acgattctga tgattgccca tagactcagt
      781 actgtaaaga attgcgatca ggtcattgag cttggcgcag gacagattcg tcgagccggc
      841 aaatacgaag aactattcgc cgctcacaat tgatcaagag aggaactatg cagattaagc
      901 tattcgacaa aatcgtcgga gacgggcagc ctgtattcgt catcgccgag gtgggactca
      961 accacaacgg ttccatggaa attgcgaagc aactgattga tgcagcaaaa gcggcaggtt
     1021 gtgatgctgt gaagtttcag aagcgtcatg tgcccagtct cgccgtgcgt tcgtttctcg
     1081 atgcccccga tttgcgtttt cctgctttcg gttcaaccta tcgagaagtg cgtcaggctg
     1141 tcgagttttc tacatcgcag ctaatggagc ttcgtgatta ttcgcgttcg aaagcgattc
     1201 ctttctttgt aactcccttt gaactgaatt ctgcgaagga gatcgatgca cttgagcctg
     1261 aagtgatcaa gagcgcgagc cacaatctta ctttcattcc gctccttgag gtgctggctc
     1321 agaaacgaaa gcctttgatc gtttctaccg gtatgtccac aatcgaagaa ctcgacatgg
     1381 ctgtgtcagt cattcgtaaa tacgattgtc ctttcgctct gcttcattgc gtgtccgctt
     1441 atccgactcc tttctcgctt gttcaacttc gaatgattga tcttcttcgt gagcgttacg
     1501 gcgttcctgt tggctattct ggccatgaag agttcagtgc gaagagcttg cctacgctcg
     1561 ctgccgtggc gcgcggtgcc tgcatagtgg aacgtcacat cacgctcgac aacaacatgc
     1621 cgggatttga tcacaagata tctcttaatc cggcggatct caaagaagtt gttttgcaga
     1681 ttagagcgat tgagtccatg atgggtgacg ggaagaaaca tctacttcct gaagagcttg
     1741 tgaagagaga gcagcaacgc gtctcgattg caactgcgcg cgcgattcgc gcaggagaaa
     1801 tcatttcatc cgagatgatt tcctttcggg ggccaggcac aggtttcgcg acgtatgaga
     1861 taaacaatgt agtcgggaaa aaagccgtcg tcgatatttc cgaagacgtt ctcattgaaa
     1921 aggcaatgct aagctaacct atgcctcgtg tttccattat cattcgagca aagaacgaag
     1981 agtactggct gccgcgtctt cttccgcagt tgaagcgtca gtcgtaccag gatttcgagg
     2041 tcattctggt ggataaccag agctcagacg caacgtgcgc catctttcgc aaggaatacc
     2101 catcgggacg aatcctcgaa attggagact ttaagccggg cagagccatc aatgcaggca
     2161 tagaagctgc gagcggggaa ctcgtggtca ttatttctgc tcactgcgtg cctgcggagg
     2221 agagctggct tgccagtttt gtttctgcga tggacgatac aaccattggc gcagcatacg
     2281 gccggcagct tcccatgcac acatcgcatc ctctcgacaa aagggacttg ctcaacacat
     2341 tcggtatcga gtttcgagta cagaagaagg acaccttttt tcacaacgcc aacagcatat
     2401 tgcgtcgctc ggtgtgggag agatttccct ttgatgaaga gacgcctcac atcgaagatc
     2461 gcctctgggc cgctcagttg attgagaatc agatatggat cgcgtacaca ccgggcgctg
     2521 tagtgtatca tcaccacggc atcaatcatc atgctaacat aaaacgagca gaagcaattt
     2581 cgcagattct tgggagtggt cgccttggtt attcgatgga gcttccagaa ttcatgcgtg
     2641 ctgagaattc acatacgctt tattgttttc tgggccatga cgcaggtact cataccgttc
     2701 ttctgtcttt gctgggccag atgaaggacg ctccgccagg cagattcatt gttcattctg
     2761 cacaggccga gcttctgcgg gatcttcccg tagggcccga cgttcttccc tacgcggcgg
     2821 acgatcgtga aaggacattc gttgaaattc tggcggagtt gatcgaggat gaagcagctc
     2881 gcaatttctt tccggacgcg gtggtctata tcaatctgcg ctctcccggg ttgaagtggg
     2941 agttgctgcg cgaagctata cattgctact acggtgacat gtatgaatcc gttttctggg
     3001 cgaacagaga gtatagcaat atctggcaga aagacaacgg ggtttacgcg caacttcgtg
     3061 ccgattatac gcgtcgcgag aagaaggaac ccgtttacat tgcgcggtat ggccttggaa
     3121 ctgtgaccgc tcctgctctc attcgatccg gcgcattaat tggaaacaaa gtcgcgatcc
     3181 ttcctctcga agacaattga ttcgcctttg tgccacagcc tgacgttcag ctaaactata
     3241 gagaaaatcg tatgactacc aaacctagaa ttcttggcat cactctggcg cgcggaggct
     3301 cgaagagcgt tccgcacaag aacataaggc ccatccttgg agttccgctc atcgcgtaca
     3361 cgattcgtgc tgcgctcgct tcacaatacc tgactcgtta tatcatttcc acagacgatc
     3421 cggagattcg cgacacggcg attcgctacg gtgccgacgc accttttctt cgtcccgcgg
     3481 agttctccac cgacacagca tcgtctgttt cggcgttgca gcatgctgtc agctgggttg
     3541 agaaagagga gaatagtaag tacgactata ttattgaaat gatgtgtacg aatccgttga
     3601 agacaactgg cgacatagat tcttgcattc aaaaactcgt cgagactggc gcggactcgg
     3661 tcattgccgt gcacaaactt gacgatcatc atccgatccg tatcaagaag attgtggatg
     3721 acctcatcgt cgatttttgt ctgccagagg ttccggagat gcggcgacag gatttgaagc
     3781 cggatgcata cattagaagt ggctccattt atgcgttgag tcgcaaacac ttaatggagg
     3841 agaatcgtcg ctttggatca aagcatagca gaccttacat tcttcctcct gaaagagcga
     3901 ttaacgtgga cacagaaatt gatttcttga tcgcagaaaa gatactcgaa aaagccgcgt
     3961 ccggaacctg acgatatgcc aagaattctc gttatcaccc ctgtgcttca catcgccggt
     4021 gtacctgagg ttctcgaatc tgcgggagaa gtcacgtacc tggacgatcc cgagccttca
     4081 gaagtaatcg gacaaatctc ttcttatgat gcgattttta cgaaccccaa caagagtcgc
     4141 gtctatatcg gcgaggagat cctgtctgca ggaaatctga aggccatttg caccgcttcc
     4201 accggcacga atcacattga taagaaagcc gccgcagctc gaaacatcac tgtgctctct
     4261 ctcacagagc aacgagaggt tatcaatagg atttcttcta cggcagagct cgcattcgcg
     4321 ctgaccatgg cgcaacttcg caaagtgcta tggagtcatg cgggtgctct tgagggagaa
     4381 tgggattaca ctcactacat tggacgccag ttgaactcac tgacggttgg tgtcattggc
     4441 tatggtcggc tgggatctct atacgctggc tactgccgcg cttttggtgc gcaagttctt
     4501 gtttacgatc catacaagaa ggttgatagg gccgatattt cccagcttgc aacgatcgaa
     4561 gaattgttcg agagaagtga cgtgatttcg attcatgtgc acgtttcccc ggagaccacg
     4621 aacatgattc atgcggcgag ttttgctcgc atgaaaaagg atgttctgct catcaatacc
     4681 gcgcgtggtg agatcatcaa cgagtctgat ctggttgggt ttttgaagaa caatcctgag
     4741 gccagggcag gagtggatgt tctggcggat gaaatcaaga atcgtctgaa tagtccttta
     4801 ttggattacg caaagtccgg aaggcaagtc gtgattacac aacacatcgg cggaatgacg
     4861 cgcgaagctc aggagatcgc atacgggcat gctgctcgta tgctgcattc tttccttcgc
     4921 ggatctgcca atcctgtctg attctccttt tatgaaatac atttccgaaa tcgatagcat
     4981 ccgggctttc gcctgtctga tggtgatgtg ggatcacctc tattccaggt tcttcttgcg
     5041 cattggcgat cgagagctgt tcacgggtgg tttcatcggt gtagatgttt tctttgtact
     5101 gtctggcttt ctcatttcga gtttgttgtt tcaggaaaag gaaagaagcg gtcgcattcg
     5161 ttacggaaag ttttatgtac gcagagctct tcgcctcctg cctccgatct ttaccgtcgt
     5221 tccactcgtt ctattgccat tgatttacta cttcgacggc gcggccgtaa ccatattgaa
     5281 ctatttttac cttgtgacgt acaccacagt ccttccaaaa tttcttgagg tcactcacag
     5341 gtttccaaat ccttactggt ttccgcacgc atggtcttta tcgatcgaag aagcatttta
     5401 cctcgtgttc ccttttctgg tagggcagat ccaaaacctg aaacgattct cctggtcgct
     5461 tctttttttc ttttttctga acgcctgtat gatgccctac gtgctgc
//
LOCUS       DAORFF010000230         5501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8133_length_5501_cov_3.383033, whole genome shotgun sequence.
ACCESSION   DAORFF010000230 DAORFF010000000
VERSION     DAORFF010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8133_length_5501_cov_3.383033"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PKK76_10255"
     CDS             <1..590
                     /locus_tag="PKK76_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL68899.1"
                     /translation="FDLLARFRRNSRISSIPCIFLTGQTGSNSILQALRSGIDDYITK
                     PFHSEELADRIETIVRKKKPSVHEESLFVHFCDAHLLRSSSAAGDFDFYSSVFRRACQ
                     MISCATPEVGLFFLSADTAQISPCSREKKVEARVVRVLAAASGMMQKRADVLIRRGDY
                     LEPLAIRGLRARYSAGRDRSIVEQWLLAQNELLHS"
     gene            complement(558..1145)
                     /locus_tag="PKK76_10260"
     CDS             complement(558..1145)
                     /locus_tag="PKK76_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68900.1"
                     /translation="MTPEAIKSYKAVFAECLTSIGEQVIPEIIQHLETIQDDAAAVRS
                     ARQDKKPGAPSHWITLLQFIKLGLVVSGELKAHYDELMGTYQTLTGRESRDENENKML
                     TEVGEFLYATDEATGDLDRINLRLMKSIRNEMIDQYGIVLHPNEAIYADRIPDENFER
                     LAPMIQELTGCCDRLLDLVRVMSALLRMQKFILSE"
     gene            complement(1142..2557)
                     /gene="argH"
                     /locus_tag="PKK76_10265"
     CDS             complement(1142..2557)
                     /gene="argH"
                     /locus_tag="PKK76_10265"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNL68901.1"
                     /translation="MGPSALKLWVGRQSGGSDDILLELGSSIELDIELYREDLAGSRA
                     HAKMLGKIGILTERELADIQRGLAQIEREIEEGKFEIKHELEDIHTHIETRLTQIIGP
                     SAGKLHTARSRNDQIAVDTHLYVRKSALLIGRQILDFCSALLDSAKKEIDTILPGYTH
                     LQVAQPIRLSHHLLAHFWGFLRDASRFKTAYNTANRMPLGSGAMAGVNYKTDREFVQK
                     ELGFESLYENAMDAVSTRDHIQDFLYACASLGVRASRYAEEVVLWNSVEFGFIKITDG
                     LTTGSSIMPQKKNPDTAELVRGRAGRSVANLMNLLMNLKGLPFAYNRDLQEDRLPLLD
                     SARSSMVVARALTALAGGIEFRRDRLTRSLSEGFALATDLADALVIEKGVPFREAHHI
                     AGRLVGLCAERGFTLENAPESLRAEASDLLADESFYKKAIDMTSSTDRKASRGGTARE
                     RQEEQIGIAQGELIRVRESLS"
     gene            complement(2539..3366)
                     /locus_tag="PKK76_10270"
     CDS             complement(2539..3366)
                     /locus_tag="PKK76_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68902.1"
                     /translation="MNELTSCLPPVLIRNQDAIRENLETALGTLPTDLALSFESDLKR
                     AADGSLTRWLFQMICLHSGFASVTLFAGPIAIFSYGIQSDSRTMPPDVWTEIDLPFIV
                     GEHRLRFYGILPIKNKPFATQASDSLLVHAENVFLNSHGLREKSIQNIFFHVEKELRP
                     LLEALLEQGTPVTLTHFLFQDLRVYFSLSGEYRSGEIMDSIQKTIKENLKNTDSIFRI
                     SPLSYIVLSPGADAAQIETRFKSIYVQIKSLVLDYHLKLFTIQRMPVRFSQIWGHLL"
     gene            complement(3385..4371)
                     /gene="cysK"
                     /locus_tag="PKK76_10275"
     CDS             complement(3385..4371)
                     /gene="cysK"
                     /locus_tag="PKK76_10275"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNL68903.1"
                     /translation="MKIFEDNTLTIGRTPLVQLNRVAAGKGARVIAKIEGRNPAYSVK
                     CRIGAAMIWDAEKKGKLKKGMEIVEPTSGNTGIALAYVAAARGYPLTLTMPESMSIER
                     RKVLKAFGAKLVLTEAAKGMKGAIEKATEIANSDSAKYFMPQQFDNPANPAIHEETTG
                     PEIWQDTEGKIDIFVAGVGTGGTITGVGRYLEKQNKAIQIVAVEPAHSPVLTQTMKGE
                     KVAPGPHKIQGIGAGFVPSIVDISLIDRVEQVTNDESIEMARRLAKEEGILCGISCGA
                     AATAALRLASLPENAGKNIVVILPDSGERYLTTALFEGLFSGIDAAAAGAVI"
     gene            complement(4406..>5501)
                     /locus_tag="PKK76_10280"
     CDS             complement(4406..>5501)
                     /locus_tag="PKK76_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNL68904.1"
                     /translation="ELHCAHSQPVWSQSMSANKKSPVARRPSPGKLPAKEKAKKPSTQ
                     ADTKFARNLKKLLSLIPILKANQGIKIKDLQKISGYTSEKKLHEDLEKLLFFGQPPFT
                     PSDYIDIYIEDDRVFLEFPQGLDRPLALTPDEWTAVQALLEKEINFYSGGRKPTKHLR
                     DIVLRMSQVPLAIEPGPFARKQSILMRAIDDSLQVEFQYRSLYSKEPEIRRIDPWAVF
                     ANQGTIYCLGYCHTRRSPRIFHLERMEKVEILNVKQEKAPSVQPVLNESFIFRKDKSG
                     FSGEIEFDRGVRPALERTLDLKEIKKGAGGRWTAKVNIKDSHWFKAMIRSFGPYVKIL
                     SPDHLRQSFISELREIPVPEAFEVDGKSNS"
BASE COUNT         1193 a         1347 c         1393 g         1568 t
ORIGIN      
        1 ggttcgacct ccttgccaga ttcagacgca attcgcgcat ctcttctata ccgtgcattt
       61 ttctgacggg ccagactgga tcaaattcca tcctccaggc tctgagaagc ggaatcgatg
      121 attatattac gaagcctttt cattcagaag agcttgcgga tagaattgaa accattgttc
      181 ggaagaagaa accgtccgtg cacgaggagt ctttgtttgt ccacttctgt gatgcgcatt
      241 tactcaggtc gagcagcgcg gcgggcgatt ttgactttta cagttccgtg tttcgtcgcg
      301 cctgccagat gatttcctgc gccacgcccg aggtcggcct tttctttttg tccgcagata
      361 cggcgcagat ttctccttgc tccagggaga agaaggtgga ggccagggtg gtgcgtgtgc
      421 tcgccgctgc gtccgggatg atgcagaaac gtgcagacgt tctcattcgt cgtggcgatt
      481 atctcgagcc gcttgccatt cgaggacttc gtgccaggta ctcggcaggc agagaccgat
      541 cgatcgtaga acagtggtta ctcgctcaga atgaacttct gcattcttag aagagcgctc
      601 atcactctga ccagatccag caatctatcg cagcatccag tcaattcctg gatcatgggt
      661 gccagtcttt caaagttttc gtccgggatc ctgtccgcgt agatggcctc gtttggatgc
      721 agaacgattc cgtactgatc gatcatttca tttctgatcg atttcatcag tcgcaggttg
      781 atgcggtcca gatctcccgt tgcttcatcc gttgcgtaga gaaattctcc tacttcagtg
      841 agcatcttgt tttcgttttc gtctcttgat tcccgcccgg tcagagtctg gtatgtgccc
      901 atgagttcgt catagtgcgc cttgagctct ccagaaacaa cgagcccgag tttgatgaat
      961 tgcaggagag tgatccagtg cgacggtgct cctggcttct tgtcttggcg agcactgcgt
     1021 actgctgccg cgtcgtcttg aatggtttcc aggtgctgga tgatttcggg gatcacctgt
     1081 tctccaatgg aggtcaggca ttctgcaaac actgccttat agcttttgat cgcttccggc
     1141 gtcatgaaag actttcccga acgcgaatca gctctccttg tgcaattcca atctgttctt
     1201 cttgcctttc gcgcgcggtt ccgcctctgc ttgcttttct gtccgtgctc gaagtcatat
     1261 cgattgcttt tttatagaat gattcatccg ccagtaagtc cgaggcttcc gctcgtagcg
     1321 attcaggtgc attttcaaga gtgaatccgc gctctgcgca caatccaacg agtctgcctg
     1381 cgatatgatg agcttctctg aacggaacgc ctttttcaat gaccagcgcg tctgccagat
     1441 ctgtggcaag agcgaacccc tcggaaagag aacgcgtcag gcgatcacga cgaaattcaa
     1501 ttcctccggc aagagcagtc agtgcgcggg ccactaccat ggatgatctg gcggaatcca
     1561 gaagtggaag tctgtcttct tgcaggtcgc gattgtatgc gaaaggaagt cccttcaaat
     1621 tcatgagcag attcatgaga ttggcaaccg accttccagc gcgaccgcga accagctctg
     1681 ctgtgtccgg attttttttc tggggcatga tgcttgaacc tgtggtcagc ccgtctgtga
     1741 ttttgatgaa accaaattct actgaattcc acaaaactac ttcttctgcg tatctggatg
     1801 cacgcacacc cagggacgcg catgcgtaaa gaaaatcctg gatgtgatct cgagtggaga
     1861 ctgcatccat tgcattttcg tagagagatt caaatcccag ttctttctga acgaattctc
     1921 tatctgtttt gtagttcaca ccggccatag cccccgatcc aagaggcatt ctgtttgctg
     1981 tgttgtatgc ggttttgaat ctgcttgcat cgcgcaaaaa tccccagaaa tgggccagca
     2041 ggtgatggct cagacgaatg ggttgagcga cctggaggtg agtgtatccg ggaagaatcg
     2101 tatcgatttc tttcttcgca gaatcaagga gggcagagca gaaatccagg atctggcggc
     2161 caatgaggag agcggacttt cgcacgtaca gatgagtgtc cactgcgatc tgatcgtttc
     2221 gcgatcttgc tgtgtggagt tttccggcgg aagggccaat gatttgagtc agccttgttt
     2281 ctatgtgcgt gtggatatct tcgagttcgt gtttgatctc aaattttccc tcttcgattt
     2341 ctctctcaat ttgtgcgaga cctctttgaa tgtccgcgag ttccctttca gtcaggattc
     2401 caattttgcc cagcatcttc gcatgagctc tggaccctgc gagatcttcg cgatacaatt
     2461 caatgtccag ttcgatggag cttccgagtt caagtagtat atcgtctgaa ccgccagact
     2521 ggcgtcccac ccagagtttc aaagcagatg gccccatatt tgagagaatc gcaccggcat
     2581 tctctggatt gtgaatagtt ttagatgata gtccaggacc aggctcttga tttgcacgta
     2641 aatgcttttg aatctggttt caatctgagc tgcgtctgct cctggagata aaacaatgta
     2701 gctgagagga gagattcgaa atatactgtc tgtattcttt aaattttctt taatagtttt
     2761 ctggatggag tccatgattt cgccgcttct gtattctcct gaaagagaaa aatatacgcg
     2821 caaatcctgg aatagaaaat gagtcagagt aaccggagtg ccctgttcga gcaatgcctc
     2881 gaggagaggg cgcagttctt tttccacgtg aaagaaaatg ttctgaatgc ttttctctct
     2941 gagtccgtgt gaattgagga acacgttttc tgcgtgcacg agaagtgagt cgcttgcctg
     3001 agttgcgaac ggtttgtttt tgatgggaag gattccgtag aagcgaagtc tgtgctctcc
     3061 tacaatgaat ggtaaatcta tttcagtcca aacatccgga ggcatggtgc gcgagtcgct
     3121 ctggattcca tatgagaaaa tggcaatggg tcctgcaaac agcgtgacgc ttgcaaatcc
     3181 gctgtgaagg cagatcattt gaaaaagcca gcgagtgagg gatccgtctg ccgctctttt
     3241 gagatcactt tcaaaagaaa gcgccaggtc tgttggaaga gtacccagcg cagtttcaag
     3301 attttcgcga atggcatcct gatttcgtat caggacaggg ggcaggcagg atgtaagttc
     3361 gttcactgcc ccctcccgga gtagttagat tacggcgccg gctgcagctg catcaatgcc
     3421 gctgaacaat ccttcgaaaa gtgccgtcgt cagataacgt tcgccagagt caggaaggat
     3481 gacaacgata ttcttgcctg cattctctgg caacgaagcc agtctgagcg ctgcggttgc
     3541 tgccgctccg caggaaatgc cacagaggat tccttcctct tttgccagtc tgcgcgccat
     3601 ttcaatggat tcgtcattgg tcacctgttc tactcgatca atcaggctga tgtccacaat
     3661 ggatggaaca aaacctgcgc cgattccctg gatcttgtgc gggcctggag ctactttttc
     3721 gcccttcata gtttgcgtga gtaccgggga gtgtgccggt tccactgcta caatctggat
     3781 tgccttgttt tgcttttcga gataacggcc cacacctgtg atcgttccgc ctgtgccaac
     3841 gccagcgacg aagatgtcaa tcttgccttc tgtatcttgc cagatttcag gaccggttgt
     3901 ttcttcgtgg atggctggat ttgcaggatt gtcaaactgc tgaggcatga aatacttcgc
     3961 tgagtcagag tttgcgattt ctgtcgcctt ttcgatggct cctttcattc ccttggcggc
     4021 ttctgtcaga accagtttgg cgccaaatgc tttgagaact ttccggcgct cgatggacat
     4081 gctctctggc atggtgagtg tgagcggata gccgcgagcg gctgccacat aggccagggc
     4141 aatgcctgtg tttccagaag taggctctac gatttccatt cccttcttca gctttccttt
     4201 cttctctgca tcccagatca tggctgcgcc gattcggcat tttacagagt aagccgggtt
     4261 tctcccttca attttggcga tcacgcgcgc gcctttgcct gccgctactc ggttcagttg
     4321 aaccaggggt gtgcggccaa tcgtaagtgt attgtcctcg aagattttca taaagacctc
     4381 attgtctagt gtttatatca ggatactagg aattgctttt tccgtcaact tcaaatgcct
     4441 ctggaaccgg tatctcgcgc aactcggaaa tgaagctctg tctcaggtga tccggagaaa
     4501 ggatcttcac atacggtcca aaagaacgaa tcattgcttt gaaccagtgg ctatctttga
     4561 tgttcacctt cgccgtccat ctgccacccg cacctttttt gatttctttg agatccaggg
     4621 ttcgctccag agcaggtctc acacccctat caaattctat ttctccagaa aagccgctct
     4681 tgtccttgcg aaagataaaa gattcattca gaactggttg aacggaagga gctttctcct
     4741 gcttcacgtt tagtatctct actttttcca ttctttccag atgaaagatg cgcggagaac
     4801 ggcgagtgtg gcagtatccg aggcaataga tggttccctg gttcgcaaac acagcccacg
     4861 gatcgatccg acgtatttcc ggttcttttg aatagagact tctgtactga aattccacct
     4921 gaagcgaatc gtcgatggct cgcatgagta tggattgctt tcgcgcaaat ggtccgggtt
     4981 cgatggcaag cggaacctga ctcatgcgaa gaacaatgtc tctcaaatgc ttggttggtt
     5041 ttcttccgcc gctatagaag ttgatttctt tttcgagtag agcctgaact gcggtccatt
     5101 cgtccggtgt aagagccaga ggacgatcga ggccctgggg aaactccaga aagactctgt
     5161 cgtcttcaat gtaaatatca atgtagtcag aaggagtgaa gggcggctga ccaaagaata
     5221 atagtttttc caggtcttcg tgcagcttct tttcgctcgt atagccggag atcttctgca
     5281 aatctttgat cttgattccc tgatttgctt ttagaattgg aatcagagaa agaagtttct
     5341 ttagattgcg cgcgaacttt gtgtctgcct gcgtggatgg cttctttgct ttttctttcg
     5401 cgggcagttt cccgggagag ggacggcgtg ccaccggaga tttcttgttt gcgctcatgg
     5461 attggctcca tactggctgc gaatgcgcgc aatgtaattc a
//
LOCUS       DAORFF010000231         5500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8138_length_5500_cov_3.245546, whole genome shotgun sequence.
ACCESSION   DAORFF010000231 DAORFF010000000
VERSION     DAORFF010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8138_length_5500_cov_3.245546"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="PKK76_10285"
     CDS             complement(<1..963)
                     /locus_tag="PKK76_10285"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HNL68905.1"
                     /translation="MNLQIFAIGSIYICALIRACPLFLLGPETGKLTATVVEKIAFVL
                     SVILAIMLIFKPPRKTETTERSYAENVLAIVLISTFTLTGILFSIRCMDGLILGAYDY
                     TGISLVAGGHLYGQFLSSPYYQSANGSIFYLAHHFSPILAFYSPFYYLTSSHAIYGIL
                     LQITAAIGMYAAYRFARAGLLAACAIVFLPVIYQFTISFHYEIFVLWFFFLLLGGIRK
                     KQWVQISLGLLLLLMVKEDMSVYLSLFAVYLITGKDRVERKIGAVILAVCAVSFVCVS
                     ILYMPRASGEVNSRFLTYWGVSSLSDFIFSLTPTRVAGAWIDSWP"
     gene            complement(982..1998)
                     /locus_tag="PKK76_10290"
     CDS             complement(982..1998)
                     /locus_tag="PKK76_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020779603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNL68906.1"
                     /translation="MKKRRLGTSSMVVSEIGLGTMTFGSSCDEETAFKIMDLAYESGI
                     DFFDTAEIYPVPPEAKWVHRTEEIVGKWMKGKPRDSIILATKVCGPGHGWFVPPVRSG
                     KTALDRVNIRRAIEGSLERLQTDYIDLYQTHWPDHDMRYEDTLEVLTELEEEGLIRAA
                     GCSNETAWGLMKALQVSEYHGLIKYRTIQNNFSMVNRRFEDALAQVCRKEQVSLLPYS
                     PLAGGVLSGKYNDNQVPKNARFTKYLESGERQKKQAHRFVNDKTLSSTAAFHKIAAEA
                     GIHPIAMAVAWSKQHDFVASTLIGANTPEQLREILGGDELILSPEILAKIDEVSKSIM
                     YPMN"
     gene            complement(2038..3366)
                     /locus_tag="PKK76_10295"
     CDS             complement(2038..3366)
                     /locus_tag="PKK76_10295"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNL68907.1"
                     /translation="MIALPTKRAAILFAVPLVLSVFLLPYFILSGSNLPAAPIILGMN
                     LFFLGIFAMDWFTIPRAKRFTAERETEPVLLQNAKHSITLALIYHLKKGASVQSSIYD
                     DILPFMECKTFPLAVPISPGRNEFSYPVTVSRRGPYDLKHVHLTVFGALGLARKVYRI
                     PCETSLRVYPDLRSVSKLILLSRTSRLSFMGIRRIRTTGGDTEFERLREYTRDDEFRR
                     IDWKATARQRRFMVRQYQQSRNQTIIFMLDCGRMMTAESQGVRVLDQAISSILLLARV
                     ALDQGDRVGILAFSSRVLRFVAPVSGSGKHRVLVQACYDLYASHEETNFDLAFRHLQR
                     ASRKRALVCVVTNVIDDANADMIRSHLEVLSGRHLPFAVLLKIREIHEMAEKANDAAI
                     RLGVSDFLEWRKNVIHRLSAGGVLVLESFPDQLEVSLINQYLWIKARNLL"
     gene            complement(3363..4295)
                     /locus_tag="PKK76_10300"
     CDS             complement(3363..4295)
                     /locus_tag="PKK76_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNL68908.1"
                     /translation="MTPEIQNLRREIGKVFVGQEDLVTGVLTCLLGGGHALLEGMPGL
                     GKTLLVRTLGGVLGLDFARIQFTPDLMPTDVTGTQIYNQKNQEFVFHRGPVFTHILLA
                     DEINRSPAKTHSALLEIMQEQQITLDNQTYKIDAPFLVLATQNPIESEGTYALPEAAL
                     DRFLLKLKINYPGEQEELEILRLQTRPSVEQAIARVITRDQVLELQNKTAQIHVANSV
                     LTYINSLVRATRQRREIYVGASPRAGIALLKTARAVALLAGRTYVVPDDVKKMAHSAM
                     RHRLLLLPEVEVEGKTPDDIIDDLLATVPVPKDE"
     gene            complement(4292..5488)
                     /locus_tag="PKK76_10305"
     CDS             complement(4292..5488)
                     /locus_tag="PKK76_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68909.1"
                     /translation="MTHRFILTLLVFATFCRGPLVEEDYASIGGKSINGIDSFAEILK
                     STGRTVRTSAYFTKETATSDVIIHFEPDFGYDEEVYRNLEAWMSGLDLNPEDLTDKDK
                     VVHDSRTYAVPDTESKEAHGDPAEKTDEEETADNTNTEKEVDLKPVTLIYFLRDTTAS
                     AEFWRKLENALKDYPEERAFARYNLERTEFRESPPQGEIPTLFGFRSLLKGQKEVHQA
                     RSFSSDEIEDLPPWFPYRILHSGEPALSIEERFAYRTILLGDDHDLIREFFLKRGRVI
                     LVYNSESFLNYAQAKTAQRKFAEKFTTYALKDAPKDGTISIIQKAPVPPRTQKEKEES
                     PFRIFTVFPLNVIFFQLLVFLLVFLLSRAKVGSPLREEAPAGSRDFTEHFTALGNLIR
                     KTGTRS"
BASE COUNT         1388 a         1284 c         1375 g         1453 t
ORIGIN      
        1 tggccaggag tcgatccagg ctcctgccac tcgcgttgga gtcagggaaa aaataaaatc
       61 agataaagaa gaaacgcccc agtaagttaa gaatctggag tttacttctc cggaagctct
      121 gggcatataa agtatagaaa cacaaacaaa tgaaaccgcg catacggcca gaatcacagc
      181 tccgatcttt ctctcgactc tgtccttccc ggtgatcagg taaactgcaa agagtgaaag
      241 ataaacactc atgtcttctt tcaccatgag tagaagcaat agtccaagag atatctgaac
      301 ccactgtttc ttcctgattc cgccgagcag taaaaagaaa aaccagagaa caaatatctc
      361 gtagtgaaat gaaattgtga actgatagat gacaggaaga aatacaatcg cacacgcggc
      421 cagcagtccc gcgcgcgcaa accgataggc ggcatacatt ccaatggcgg ctgtaatttg
      481 aagcagaatc ccgtagatcg catgcgaaga ggtcagataa tagaagggag aatagaatgc
      541 gagtatgggt gaaaaatgat gcgcgaggta aaagatggag ccgtttgcac tctggtagta
      601 gggggaagaa agaaactgcc catagagatg cccgcctgca acaaggctga ttccagtgta
      661 gtcgtaggcc cccagaatca aaccatccat gcatcgaatc gaaaacaaaa ttccggtaag
      721 tgtgaatgtt gaaataagaa cgatcgcaag gacgttttct gcgtacgaac gctctgtggt
      781 ctccgtcttt cgcggcggtt tgaagatcag catgattgca aggatcacag agagtacgaa
      841 ggcaatcttc tcaacaaccg ttgcagtcag ctttccggtc tccggtccaa gaaggaacag
      901 aggacaggct cgaatcaacg cgcagatata aatggagcca atggcaaaga tttgcagatt
      961 cacccgagaa cgccttcaaa atcaattcat ggggtacatg atcgatttgc tcacttcatc
     1021 aatttttgca agtatttccg gagatagaat caattcgtct ccaccgagaa tctcgcgaag
     1081 ttgttcagga gtattggctc caatcaaagt ggaagcaaca aaatcatgct gcttgctcca
     1141 tgctacagcc atggcgatgg gatgaatgcc cgcttccgct gcgattttat gaaatgccgc
     1201 ggttgagctg agcgttttat cattcacgaa ccgatgcgcc tgtttctttt gtctttcacc
     1261 cgattccaga tactttgtga atcgtgcgtt ttttggcact tgattgtcgt tgtattttcc
     1321 gctgagcact ccccctgcca gaggagaata gggtaataga ctcacctgtt ccttgcgaca
     1381 aacctgggcc agtgcatctt cgaatcttct gttcaccata ctgaagttgt tctggatggt
     1441 gcgatatttg atcagtccgt ggtactcgga gacctgcaaa gctttcatca atccccacgc
     1501 agtttcgttg ctgcaaccgg ctgcgcgaat caacccttct tcttctagct ccgtgagtac
     1561 ttctagagta tcttcgtatc tcatatcatg atccggccag tgagtctggt agagatcaat
     1621 gtagtctgtt tgcagtcttt cgagacttcc ttcgatggcg cgccgaatgt tgacacgatc
     1681 gagagcagtt ttgccggatc ttaccggagg aacaaaccat ccatggccgg gcccgcacac
     1741 cttggttgcc agaatgatgg aatcgcgagg ttttcccttc atccacttgc ctacgatttc
     1801 ctctgtgcga tgaacccatt tcgcttccgg aggtacaggg tagatttcag ccgtatcaaa
     1861 gaaatcaatt ccggattcgt acgccaggtc catgattttg aatgccgtct cttcatcgca
     1921 ggaagatcca aatgtcatgg ttccaaggcc aatttcagaa actaccatgg agcttgtgcc
     1981 gagtctgcgc tttttcataa agaattccta ttgtttaaaa taattagtat ttcgtcgcta
     2041 cagcagattt cttgctttga tccataagta ttgattgatc aaagacactt ccagctgatc
     2101 tggaaagctt tccagaacca gaacgcctcc cgccgaaagg cgatgaatga cgttctttct
     2161 ccactccaga aaatctgaaa caccaagacg aatggctgca tcgtttgctt tttctgccat
     2221 ttcatgaatt tcccggattt tcaagagaac tgcaaatgga agatgtctgc cgctgagtac
     2281 ttccaggtga gatcgaatca tgtctgcatt tgcatcgtcg attacatttg tgaccacgca
     2341 aacaagcgct cttttgcggg atgcgcgctg caggtgacga aacgcaagat cgaaattggt
     2401 ttcctcatgg gacgcataca gatcgtaaca ggcctgcacc agaacgcgat gtttgccgga
     2461 gccggaaaca ggcgcaacaa agcgaagaac gcgcgaacta aaagcgagaa ttcccactct
     2521 gtctccctga tccagggcca ctcgcgcaag cagaagtata ctcgagattg cctgatcgag
     2581 cacgcgcact ccctgggatt cggcagtcat cattcgcccg cagtccagca tgaagatgat
     2641 ggtttgattt ctgctctgct ggtattgacg caccatgaat cgtctttgtc tcgcggttgc
     2701 tttccagtcg attctgcgaa attcatcatc tcgcgtatat tcgcgcagcc tttcgaattc
     2761 agtatctcca cctgttgtgc gaatgcggcg gatgcccatg aagctcaatc tggatgtgcg
     2821 cgaaagtaga atgagcttgc ttacacttct aagatcgggg tatacacgga ggctcgtttc
     2881 gcatggaatg cgatacactt tgcgcgcgag cccaagtgct ccgaagactg taaggtgaac
     2941 gtgctttaga tcgtacgggc cacgcctgct tacagttaca ggatacgaaa attcatttct
     3001 tcctggagag atgggcacgg ccagaggaaa tgttttgcat tccatgaaag gaagaatgtc
     3061 gtcgtaaatg gatgattgaa cggacgcgcc tttttttaaa tggtagatga gtgcaagtgt
     3121 gatcgagtgc tttgcattct gcaagagaac tggttctgtt tcgcgttccg ccgtgaatcg
     3181 cttcgcgcgt ggaatggtaa accagtccat ggcgaagatt ccaaggaaga acaaattcat
     3241 ccccagaatg atgggcgctg caggcagatt gcttcctgaa aggatgaaat acggaagcaa
     3301 aaatacggac agaaccagcg ggactgcaaa cagaatcgcc gcgcgttttg ttggcagcgc
     3361 aatcattcgt cttttggcac cggcacggtt gcgagtagat cgtcaatgat gtcatccggc
     3421 gttttgcctt ctacttccac ttccggtaaa agaagcaatc tatgacgcat ggcggaatga
     3481 gccattttct taacgtcatc cggcactaca taggttctgc cggccagcag tgccacggcg
     3541 cgtgctgttt tcaaaagtgc aatgcctgcg cgaggagaag cacccacata gatttcacga
     3601 cgttgcctgg tggcgcgcac gagtgaattg atgtatgtga gtactgagtt ggccacgtgg
     3661 atctgtgctg tcttgttttg aagttccagc acctggtcgc gagtgatcac gcgcgcaata
     3721 gcttgctcta cggaaggtct ggtttgcaga cgaaggattt caagttcttc ctgttcacct
     3781 ggataattga ttttcaattt gagaagaaag cggtcgagtg ctgcttctgg cagcgcgtag
     3841 gttccttctg attcaatcgg attctgcgtt gccagtacaa gaaagggcgc gtctattttg
     3901 tatgtttgat tgtctagagt gatctgctgt tcttgcatga tttcaagaag tgcagagtgc
     3961 gtttttgcgg gagatcggtt gatttcatct gcaagtagaa tgtgagtgaa cacaggcccg
     4021 cgatggaaaa caaattcctg gttcttctga ttgtaaattt gagtgcccgt tacgtccgtt
     4081 ggcataaggt caggagtgaa ctggatgcgt gcgaaatcga gcccgagcac accgcccaga
     4141 gtccgaacca gcagtgtttt cccgagaccc ggcatccctt ctaaaagagc atgccctcct
     4201 ccgagcagac acgtaaggac tccggtgaca agatcctcct gtcccacaaa tactttgccg
     4261 atctctcttc tgaggttctg gatttccggt gtcatgatct ggttcctgtt ttgcgaatca
     4321 agtttccaag tgcagtaaaa tgttctgtga agtctctact ccccgcgggc gcttcttcgc
     4381 gcagcggaga gccaaccttc gccctggaga gaaggaatac caggaggaag acgagcaact
     4441 gaaaaaatat gacgttgagc gggaaaacag tgaagattct aaacggactt tcttcctttt
     4501 ccttttgagt tcgcggcggg acgggtgctt tctggataat cgaaatggtt ccgtctttag
     4561 gagcgtcttt gagagcgtag gtggtgaatt tttcggcaaa ttttctctgg gccgtttttg
     4621 cctgcgcgta attaaggaaa gattcagaat tgtagactag aatcacgcga cctcttttga
     4681 gaaagaattc tcgtatgaga tcatgatcgt ctccgaggag gatggtgcga taagcgaacc
     4741 tttcctctat ggaaagagca ggttctcccg agtgaaggat tctgtatgga aaccagggag
     4801 gtaggtcttc gatttcatcg ctgctaaaag atcttgcctg atggacttct ttctggcctt
     4861 tcaaaaggga acgaaagccg aataacgttg gaatttctcc ttgaggaggg ctctcgcgaa
     4921 actctgttct ttccagattg tagcgtgcga atgccctttc ttctggatag tctttcagcg
     4981 cgttttccag ctttcgccag aattcagcag aagcggtcgt atcgcgcaag aagtagatca
     5041 acgtcacagg tttgagatcg acttcctttt ctgtgtttgt attgtctgcg gtttcttctt
     5101 cgtctgtctt ctctgcggga tcgccgtgcg cttctttgga ttctgtatct ggcaccgcgt
     5161 acgtgcgaga atcatgaacc accttgtctt tgtccgtgag gtcttccgga ttcaaatcca
     5221 gaccggacat ccaggcttcc aggttgcgat atacttcttc gtcgtaaccg aagtctggtt
     5281 cgaaatgaat gattacatct gatgttgcgg tttcttttgt aaaataggca gacgtgcgaa
     5341 cggttcgtcc cgtggatttt aagatttctg cgaagctgtc tatgccgttg atactcttgc
     5401 caccaatgga tgcataatcc tcttctacaa gaggaccccg gcagaatgta gcaaagacca
     5461 gtagtgtaag aatgaatctg tgagtcatca aaagaggcta
//
LOCUS       DAORFF010000232         5468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8205_length_5468_cov_4.133752, whole genome shotgun sequence.
ACCESSION   DAORFF010000232 DAORFF010000000
VERSION     DAORFF010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8205_length_5468_cov_4.133752"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PKK76_10310"
     CDS             <1..941
                     /locus_tag="PKK76_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNL68910.1"
                     /translation="FGNLAPRDVASRQAKYVCDEGRGVGDTGLGVYLDFKDAIARLGE
                     STIRERYGNLFQMYERITGEDPYKRPMRIYPAVHYTMGGLWVDYNLMSNLPGLFVIGE
                     ANFSDHGANRLGASALMQGLADGYFILPYTIANYLAQVGPAKLPEDHAEFKKSEAEVT
                     EMTNKFLSINGTRSVDSFHKELGKILWDHCGMGRNEAGLKKAIARIPELREEFWKTVR
                     VPGSGSSLNPEIEKAGRVADFLEFGELMCRDALHRNESCGGHFREEYQEDGEAKRNDD
                     KFCYAAAWEYNGKSEPKLNKEPLVFENIHLATRSYK"
     gene            952..1734
                     /locus_tag="PKK76_10315"
     CDS             952..1734
                     /locus_tag="PKK76_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNL68911.1"
                     /translation="MNLTLHVWRQKDKDDPGRMVEYKVGEAEGVNEHMSFLEMIDVLN
                     ERLISQKEEPIAFDHDCREGICGACSMMINGVPHGPEKGTTTCQLHMRKFKSGDTIYI
                     EPWRAKAFPVIKDLVVDRSGFDKIIQAGGFVSINTGNAVDANCLPIAKTDSDLAMDAA
                     QCIGCGACVATCKNASAMLFVAAKVSHLGLLPQGQPERDRRVLEMVAVMDEAGFGNCT
                     NQFECEAACPKEISVDFIARLNRDYARAVARERPARQEMGAG"
     gene            1735..2850
                     /locus_tag="PKK76_10320"
     CDS             1735..2850
                     /locus_tag="PKK76_10320"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL68912.1"
                     /translation="MRMRALISGMFLLVACRQELPDDRSLLLSLLSLRYFGPRFFVVG
                     YGSPSHAEVYSVNGDGQVTFVSQATGGNFPVMLSADPTLNYIRMGSNSGGGYLTSFSL
                     NRSTGSLAQVHEIPATGASLATADHPSLPFVMNVVTGQVNVHPVLSGGVLGTAVGYDA
                     GRTFSGARLYGNILYAADNSAAGTVRSYRLGSTGVLTTLQTMPSSATNQATVGIEPLG
                     RFMYVSNGSATNQVALFSLDGNGSMSGPTFMTLSNGAGGTNFAFDPYGRYLFASRSIA
                     TNNLQTYAINQATGQLTLASMITSGTGPSQIVVDPAGRFLVHLDAAGVTMRVYSYSNG
                     MLTPVTGSPFSIGPGTNPVALIAICYEKYAFGELQFN"
     gene            2935..3573
                     /locus_tag="PKK76_10325"
     CDS             2935..3573
                     /locus_tag="PKK76_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68913.1"
                     /translation="MSQIALPPPVAPSTGINRFFVVSFVIHTLIAGGLYLYLYAWPAE
                     EHYVATVALYMGDAENDRETTGKGKGNKDASKDDKKTSSDTGTGPSTSSNADWGTASD
                     PSTEGGSRYAPDLWIDGNLEDFYPARAKQANLGKVTVRVSLYIDSTGKIREVRVQGVR
                     SAGNAHAPYEADFRQAAQQIALTRMRLRSAGYRKDGRPVDFIWDTVINFTLQ"
     gene            complement(3602..4210)
                     /locus_tag="PKK76_10330"
     CDS             complement(3602..4210)
                     /locus_tag="PKK76_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNL68914.1"
                     /translation="MRRKEFVVEDATEIEQFLGEMSFGFLTQNRGEVPAITPLNYVVY
                     RGDICFHGSRVGEKMQLLKAGTAAAFCVAREVSIIPSFFSGAEIACPATAFFKSVVIR
                     GSLEEVTDLQEKCDILTALMEKLQPEGRYAPFDLEDPEYLKNVKGVSVVRLKTQERTA
                     KFKFGQNQTRTKWQQILDNLLRRGRPSDLEAASEMKKRCPFH"
     gene            4254..>5468
                     /locus_tag="PKK76_10335"
     CDS             4254..>5468
                     /locus_tag="PKK76_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNL68915.1"
                     /translation="MKRSKIDNIYWTSFQEVSSYRPMIPLGPYLARHPRKQDALYHGL
                     RDVILGGSVEPDSRLPSTRTMARQLGLSRGTVVLAYEMLESDGLIRLDAGRAPVVLHR
                     AQERITVKAAPYKASLWASRLPVEEKSRKENETRTSKRIVDFNPGYTDPELFPAREWN
                     QALHRAIRTFDPQVAEAAGGLVTLRRGVASYLSKRRGMVVHADQIVIVNGSIQAIVIL
                     SHILLGENSKAVLENPGYRGIRDAVRLCGASPIFAAVDKEGIRVRDWDAQLCFVTPAR
                     QFPTGAALSYDRRLELVEWAERKNAFILEDDYDSEFRRRGRPLEPLQCLAPARVVHVG
                     TFSRTLAAGLRLGYAVLPENLLDPFLRAKRTFETHPAGTIEQSALSELMRSGAYERHL
                     RRAARVYSRRHDL"
BASE COUNT         1302 a         1365 c         1474 g         1327 t
ORIGIN      
        1 gttttggaaa cctggcacct cgcgacgttg catcgcgtca ggcaaaatac gtatgcgatg
       61 aagggcgcgg cgttggagat acaggtctgg gtgtttacct ggatttcaaa gatgccattg
      121 cgcgtctcgg agaaagcaca atccgtgaac gctatgggaa cctctttcaa atgtatgagc
      181 gcataacggg tgaagatccg tacaagcgtc ccatgagaat ctatcctgcg gttcactata
      241 caatgggtgg actctgggtg gactacaatt tgatgagtaa cctgcccggg ctttttgtaa
      301 tcggggaagc aaacttctct gatcacggcg ccaatcgtct gggtgcgagt gcactcatgc
      361 aggggcttgc ggacggatac ttcatcctgc cttacaccat tgccaactac ctggcacagg
      421 tcggcccggc aaaactcccg gaagatcacg ccgagttcaa aaagtcggaa gcagaagtca
      481 cggagatgac aaacaaattc ctctccatca acggaactcg ttctgtggat tccttccata
      541 aggaactggg gaaaatcctc tgggatcatt gcggaatggg aagaaacgaa gctggcttaa
      601 agaaagccat tgcacgcatc cctgaattgc gcgaagaatt ctggaagacc gtgcgtgttc
      661 ctggcagcgg gtcttcactg aatcctgaaa tcgaaaaagc agggcgcgtg gcagacttcc
      721 tggaatttgg cgaattgatg tgccgtgatg cacttcatag aaacgaatca tgcggtggtc
      781 acttccgcga agagtatcag gaagacggcg aagcaaagcg caacgacgac aaattctgct
      841 acgcggccgc gtgggaatac aacggtaagt cggagccaaa gctcaacaaa gagcctctgg
      901 tattcgaaaa cattcatctc gccacgagaa gttacaagta aggagccata tatgaattta
      961 actcttcacg tctggagaca gaaagacaaa gacgatccgg gccgcatggt cgaatacaag
     1021 gtaggagaag cggaaggtgt caacgaacac atgtcatttc ttgaaatgat tgatgttctt
     1081 aatgagcgtt tgatttcgca gaaagaggaa cccattgcat tcgatcacga ctgtcgcgaa
     1141 ggtatttgtg gcgcatgcag catgatgatc aatggagttc cccatggtcc agaaaaaggg
     1201 accacaacgt gccagcttca catgcgaaag ttcaagagcg gggatacgat ctacattgaa
     1261 ccatggcgtg cgaaggcgtt ccctgtgatc aaagatcttg tagtggatcg gtcgggcttt
     1321 gataaaatta ttcaggccgg cggatttgta tcgattaaca ccggtaatgc ggtagatgca
     1381 aactgtctgc ccatcgccaa aacagattct gatctggcta tggatgcagc gcagtgcatt
     1441 ggatgtggag cgtgtgtggc tacctgcaag aatgcaagcg ccatgctttt tgtcgccgca
     1501 aaggtttcgc atctgggctt gcttcctcaa gggcagccag aacgcgatcg ccgcgttctc
     1561 gagatggtcg cagtgatgga cgaggctggc ttcggaaatt gcacgaacca gtttgaatgc
     1621 gaggcagcat gtcccaagga aatcagtgtg gattttattg cccggctcaa tcgtgactat
     1681 gcgcgtgcgg ttgcacgcga acgtcccgct cgccaggaaa tgggagccgg ctagatgcga
     1741 atgcgggctc tgatttcagg aatgtttctg ctggttgctt gccggcagga gcttcctgat
     1801 gaccggagtc tcctgctttc cctcttgagt cttcggtact tcgggcctcg ttttttcgtc
     1861 gtaggatatg gatcgccttc gcacgcggaa gtgtattccg taaatggtga tgggcaagtg
     1921 acttttgttt cgcaggccac cggcggaaat tttcccgtta tgctttctgc ggatccaaca
     1981 ctgaactaca ttcgcatggg ttcaaacagt ggcggcggtt acctcacaag cttttcactc
     2041 aatcgttcca caggcagtct cgctcaggtg catgagattc cagcaacagg tgcctctctg
     2101 gccaccgccg atcatccatc attgcctttc gtcatgaatg tggtgactgg tcaagtgaac
     2161 gtccatcctg tgctttccgg tggcgtgctg ggaactgctg tcggttacga tgcaggccga
     2221 actttttcag gcgccaggtt gtatggaaat attttgtatg cggctgacaa ctctgctgcg
     2281 gggactgtaa gaagctatcg ccttggaagt acaggtgttc tgactactct tcagacaatg
     2341 cccagttccg ctaccaatca ggcaacggtg ggcattgaac cacttggtcg tttcatgtat
     2401 gtttctaacg gcagcgccac aaatcaggtc gcattgttta gtctggacgg aaatggctcg
     2461 atgagcggac caacgttcat gacactcagc aacggagctg gtggaacgaa tttcgctttc
     2521 gatccctatg gcagatatct gtttgcatca cgcagcattg caacgaacaa tttgcagacc
     2581 tatgcgatca accaggccac gggccagctg actcttgctt ctatgatcac atcgggcact
     2641 ggcccttcgc agatagttgt ggatcccgcg ggccggtttc tggttcatct cgatgcggcc
     2701 ggtgttacca tgcgtgtgta ttcctattcg aacgggatgc tcaccccggt cacgggttcc
     2761 ccgttttcta ttggaccagg cacaaatcca gtggccctga tcgcgatctg ttatgagaag
     2821 tatgctttcg gagagcttca gttcaattga tcgcgcccgg ctcaaaacgc atcgtcaaac
     2881 catcctgatc ttttttattt accgtaaagt ggcatcgcgg agaaaggaat aggtatgtct
     2941 cagattgctc tgccaccgcc cgttgcgcca tccaccggca tcaaccgttt cttcgttgtt
     3001 tcttttgtga ttcataccct cattgcgggc gggttgtatc tgtatttgta tgcgtggccc
     3061 gcggaagaac attacgtggc cactgtcgcc ctctacatgg gtgacgctga aaacgacaga
     3121 gaaactacag gaaagggaaa agggaataag gatgcgtcga aggacgacaa gaagacatcc
     3181 agcgatactg gcaccgggcc ttccacgagt tcgaacgcag actggggaac ggcatcagat
     3241 ccatctacgg aaggcggcag tcgttatgcg cctgacctct ggatagacgg aaacctggaa
     3301 gatttttatc ctgcgcgtgc gaagcaggca aaccttggca aggtaacggt tcgcgtttct
     3361 ctctacatag attccaccgg caagattcgg gaagtgcgcg tacagggagt gcgttctgct
     3421 ggaaatgctc acgctccgta tgaagctgac ttcaggcaag cagcgcagca aatcgcgctt
     3481 acaagaatgc gcctgcgcag tgctggttat cgcaaggatg gccgtcctgt ggattttatc
     3541 tgggacactg tcatcaactt tactctgcag tgattctatc cggacacgga tgtagatccc
     3601 gtcagtgaaa tgggcacctt ttcttcattt cagacgcggc ttctagatcc gagggtcttc
     3661 ctcttctcag caaattgtcc agtatctgct gccatttggt tctggtctgg ttttgcccga
     3721 atttgaattt tgcagtcctt tcctgggttt tcaaacgcac aacggacaca cctttcacgt
     3781 ttttcaagta ttctggatct tcaagatcga atggcgcata acgtccctcg ggttgaagtt
     3841 tttccatgag tgccgtcagt atatcgcact tctcctgcag atccgttact tcctccaggc
     3901 taccccggat caccacagat ttgaagaatg cggttgcggg acacgcaatc tccgcgccac
     3961 taaaaaatga aggaatgatg ctgacttcgc gcgcgacgca gaaagcggcc gccgtacccg
     4021 ctttgagcaa ctgcatcttt tcgccaactc tgctgccgtg aaagcaaatg tctccccggt
     4081 acactacgta gttcaaagga gtgatggcag gcacttcgcc acgattctgc gttagaaacc
     4141 cgaaggacat ctctccgaga aattgttcga tctctgtggc gtcttctacc acgaattctt
     4201 ttcttctcat gctgattgct ccagaaccag aagaatatca taaaactgga ccaatgaaaa
     4261 ggtccaaaat agacaatatt tactggacca gtttccagga ggtctccagc tataggccaa
     4321 tgattcccct gggtccctat ctggcgcgcc acccgcgcaa acaggacgca ctctaccatg
     4381 gattaaggga cgtgatcctg ggaggcagtg ttgagccaga cagtcggctc ccttccacgc
     4441 gcaccatggc acgacagctg ggactatcaa gaggcacagt agttctggcc tatgaaatgc
     4501 tggaatcgga tggcctgatt cgtttggacg cgggccgggc ccctgtggtt ctgcatcgag
     4561 cgcaagagcg cataacggta aaggcagccc cttacaaggc cagcctgtgg gcgtctcgat
     4621 tgccagtaga ggaaaaatcc agaaaggaaa acgaaacgcg cacttcgaaa agaatcgttg
     4681 attttaatcc ggggtacact gatcccgagt tgtttcctgc gcgagagtgg aatcaggctc
     4741 ttcaccgcgc cattcgaacg tttgacccgc aggtcgcaga agccgcggga gggctcgtca
     4801 cattgcgccg aggcgtggcc tcctacttga gcaagagaag gggaatggtg gtgcacgcgg
     4861 atcagatcgt cattgtgaat ggatctattc aggcgattgt cattttgtct cacattctgc
     4921 tcggtgagaa ttcgaaagct gttcttgaaa atcctggata tagaggcatc cgtgatgcgg
     4981 tcaggctttg tggtgcttct cccatttttg cggccgtaga caaagaagga attcgagttc
     5041 gggattggga cgcgcaactc tgctttgtaa ccccggccag acaattccca acaggtgccg
     5101 ctctttcgta tgatcgcagg ctggaattgg tcgaatgggc agagagaaag aatgcattta
     5161 ttcttgaaga tgactacgat agcgaattca gaagaagagg aagaccactc gagccattgc
     5221 agtgcctggc tccggctcgc gtcgttcacg ttggaacatt ctcgcgtaca cttgctgcgg
     5281 gactcaggct gggttatgcg gttctgcctg aaaatctact cgatcctttc ttgcgcgcga
     5341 agcgcacatt tgaaacacat cccgccggga ccattgaaca aagcgcgctt tctgaattga
     5401 tgagatcagg ggcctatgag cggcatctca ggcgagcggc cagagtctat tccagacgac
     5461 atgatttg
//
LOCUS       DAORFF010000233         5450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8260_length_5450_cov_3.815385, whole genome shotgun sequence.
ACCESSION   DAORFF010000233 DAORFF010000000
VERSION     DAORFF010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8260_length_5450_cov_3.815385"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="PKK76_10340"
     CDS             complement(<1..1146)
                     /locus_tag="PKK76_10340"
                     /inference="COORDINATES: protein motif:HMM:NF015425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68916.1"
                     /translation="MKHFAQILAPGGAISADRLRQTASICLQADVRSIEIGPRMNPVI
                     PAGAGVSKILARIDGIDFKMAPNVISSAPAAGFDSSNWVRESQLLEMAEEVASLNPHV
                     SISLIDAQQGLVGRTLSELNFVSCEQDEFWQPVLAPEGKRIDLGSRIATGDVVGAARW
                     YSASKKGGTDALTAFFRSTMTRSSMPAEPLPPEPSAPPYEGLSAKRGTLGFFVSSGQY
                     DCEMLEELAYLCSQSSIGKIHMTPWHSFLIKGIQEKQLDLWRNFLLRKGIETRHAGSS
                     LYWRFSNGRSNQSNRIHRKIIHQLGSTDKSTGQIPFVVGKDLEGQPALLGIEPTSLFR
                     CIIYKRDNLNEPWDKHGTFLEAFAAREIQIICDQRARNQKQRSGENAA"
     gene            complement(1143..4643)
                     /locus_tag="PKK76_10345"
     CDS             complement(1143..4643)
                     /locus_tag="PKK76_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNL68917.1"
                     /translation="MLSGTRTTCSYCGVGCGIVVREHKGTLQVEGDPEHPVNRGMLCS
                     KGRTLGYTIADKTDRILYPRLRASRGHSQSRVTWDTALNRAANVFRSITEKYGPDSVG
                     FYVSGQLLTEEYYIVNKLTKGFIGTNNIDTNSRLCMSSAVTAQKMAFGEDCVPINYED
                     IELADLYLIAGANPAWCHPILFRRMEEKGERVRKVVLIDPRKTDSASLADLHLQLRPG
                     TDVALYNAIARILIEKGNIDSNFISNHTENYSTLEEAALSISVREAAEICDLSASDIE
                     KCADWIADSSGFLSFWAMGLNQSAQGVRKNLSLINLHLITGHIGKPGSGPFSLTGQPN
                     AMGGREVGGLATMLAAHRDIGNQAHREEVKTFWNSKDISPKPGRTATEMFDALRSGEM
                     KAIWIACTNPIVSLPDSMAVQEGLEKARFVVVQDISERSETVKYADLVLPAAGWLEKE
                     GTMTNSERRITYLPKLIEPPGEARPDSEIWGDFARRMGYADAFAYKSAEEIYAEHCAL
                     TRGTNIDVSGLSYDRLKQSSMQWPVPHPDHPGTPRLFQDLKFHTQSGRAKIHGFRFEN
                     RSEALSAEYPLVLTSGRIRDQWHTMTRTGKVNKLGTHIPEPYVEINPEDALSRGIAEG
                     DLAEVHSKRGSVRLRARLDERIRPGLVFVPMHWGSTIGDDGTRANNVTSPALDDFSKQ
                     PDLKYAAVQVRKPVFTRRKVVIIGAGASAFRFLEEYRRMNQTDDITLICAESESFYNR
                     ILLPDVISGRKEKDSLSLVDAAQVTEWKLKLATETRATSIDRISRTVLDSRGQSHPYD
                     ILVLATGSRAILPPRLAGLSGVHTLRKLADAEQILADAKGPVVVLGGGVLGLETAGAL
                     SERGLSVTVIHRSSALMSRQVDATTGRLLRSAMIDRGIQTIVNDSIAACRGVDRIDSV
                     RLTSGRIVDCETLICAAGTEPDIDLARDAGLACESGVCVDDRMQSSDPHIYAIGEAAE
                     HRGTLYGTTLAAEDQGRTAARALCGDPGAVYSGSIRMNILKVEGVRLASIGRVEASHP
                     GQEELVYTDESAGIYKKCVVEGDRLIGCILFGDLSEMEQFRALIASRTELGELRRSLL
                     MRFEEIAPLKGDLVCSCNSVGKGNIEDCISAGITDFKELTAKCKAGTGCGSCRPEVAR
                     ILAASLENEKAPKENEERVA"
     gene            complement(4637..>5450)
                     /locus_tag="PKK76_10350"
     CDS             complement(4637..>5450)
                     /locus_tag="PKK76_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68918.1"
                     /translation="FGSFGGSGQMAGTKTLFLQNAALIWIPFIVIAFGTTWFFMNNLH
                     VSRSSVKEQLTMLKDKNAWIMSLLYMGTFGSFIGYSAGFPLLIKSQFPNVNPLQFAFI
                     GPLVGSVIRPVGGWLGDKIGGARVTFWNFAVMGLAVSGVIYFLSIKTEPGAFYGFFAM
                     FLILFVAAGIGNGSTYRMIPIIYMRKNNAHEGATATTESLNRANRQSGVVVGITSAIA
                     AYGAFFVPKSYGTSIELTGGPSGALILFLAFYIGCLAATYYFYYRKKAAYPC"
BASE COUNT         1187 a         1446 c         1398 g         1419 t
ORIGIN      
        1 tgctgcattt tctccggatc gttgcttttg attgcgagct ctttgatcac agatgatttg
       61 aatctcgcgt gctgcgaatg cttcgaggaa tgtaccgtgc ttgtcccatg gttcgtttag
      121 attgtctctc ttgtaaatga tgcagcgaaa caaactcgtt ggctcgattc caagcagtgc
      181 cggctgtcct tccaggtctt ttcccacaac gaatggaatc tgccctgttg atttgtcagt
      241 ggatccaagc tggtgaatga tctttctgtg aatgcgattg gattgattgc ttctgccgtt
      301 tgagaatctc cagtacaaag aagaacctgc gtggcgagtt tcgattcctt ttcgcaaaag
      361 aaaatttcgc cagaggtcta actgtttttc ttgtatccct ttgatcaaga aggagtgcca
      421 gggagtcata tgaatcttgc ctatgctgct ctggctgcaa agataggcga gctcttcgag
      481 catctcgcag tcgtactgtc cgctggagac gaagaacccg agtgtgccac gtttcgcgct
      541 gaggccttca tatggtggcg ccgagggttc aggagggaga ggttcggcag gcatggagga
      601 tcgggtcatc gtgcttcgaa agaatgccgt gagtgcgtct gtgcctccct ttttagaggc
      661 ggaataccag cgtgccgcgc caaccacatc gcctgttgcg attctgctcc ccagatcgat
      721 cctttttccc tctggagcaa gcacaggttg ccagaactcg tcctgctcac aagaaacgaa
      781 gttcaattcg ctcagagttc tgcccaccag accctgctgt gcatctatga gcgaaatact
      841 cacgtgcgga ttcaaagagg ccacttcttc tgccatttcc agaagctgac tttctcgaac
      901 ccagtttgag gagtcaaagc ccgcagcagg cgcagaagag atgacgttag gcgccatctt
      961 aaaatcaatc ccgtctatcc ttgcaagaat ttttgagaca ccggcaccgg ccggtatgac
     1021 tgggttcatt cttggtccaa tttcaatcga gcgtacatcc gcctgcagac aaatgcttgc
     1081 cgtttgtctc aaacgatctg cagagatcgc accacccgga gcaagaatct gtgcaaagtg
     1141 cttcatgcaa ctctctcctc gttttccttt ggtgcttttt cattttcaag agacgccgcc
     1201 agaatcctgg ccacttcagg ccgacagctt ccgcatcctg ttccagcctt acattttgca
     1261 gtgagctctt taaagtcggt gattccggca gagatacagt cttcgatgtt tccttttcct
     1321 acggaattgc aggagcatac tagatctcct ttcagtgggg cgatttcttc aaatcgcatc
     1381 aagagagagc gtcgcaattc cccgagttcc gtgcgagatg caatgagagc gcgaaactgt
     1441 tccatttcgc tcaaatcgcc gaacaggatg cagcctatga gacgatcgcc ctccacgacg
     1501 cacttcttgt aaatgccggc ggattcatcc gtatagacca gttcttcctg gccaggatgt
     1561 gaagcttcca cgcggccgat gcttgcgagt cgaactcctt ccactttgag aatgttcatt
     1621 cggattgatc cgctgtaaac ggcccctgga tccccgcaga gcgcgcgcgc agccgtgcgc
     1681 ccctgatctt ccgccgcaag agtagtgccg tataacgttc cgcgatgctc cgccgcctct
     1741 ccgatggcgt aaatgtgcgg atctgagctc tgcatgcggt catccacgca cactccgctt
     1801 tcgcacgcca gtcccgcatc ccgcgccaga tcaatgtcag gttcggtgcc cgcagcacag
     1861 atcaaagtct cgcagtctac aattcgtccg ctcgtcaacc tgactgaatc gattctgtcc
     1921 acaccgcgac atgcagcaat gctatcattg acgatcgtct ggattccgcg atctatcatg
     1981 gcgcttctaa gaagccgacc ggtggttgcg tccacctgcc tgctcatgag cgcagagcta
     2041 cgatgaatga cggtcacgga aagcccgcgt tcgctgagtg ctcccgccgt ttccaggccc
     2101 aggactcctc caccgagtac cacgaccggg ccctttgcat ctgccaggat ttgctctgcg
     2161 tccgcaagct tgcgaagagt gtgcactcca ctgagtccgg ccaggcgtgg aggtagaatt
     2221 gcccgactgc ctgttgcgag aacaagaatg tcgtacggat gcgattgacc cctcgaatcg
     2281 agaacggtgc gagaaatccg atctatggaa gtggcccgcg tttctgtcgc gagcttgagt
     2341 ttccattcag tcacttgcgc cgcgtccaca agcgacagac tatccttttc ttttcggccg
     2401 gagataacat ccggaaggag gattcgattg taaaaagatt ccgactccgc gcagatcaga
     2461 gtgatgtcat ctgtctggtt cattctcctg tattcttcga ggaagcgaaa tgcggatgcg
     2521 cctgcgccaa tgattaccac ttttcgcctg gtaaagactg gctttcgcac ctgcactgcg
     2581 gcatatttta gatcaggctg ttttgagaag tcatccaggg cgggagaagt aacgttattc
     2641 gcccttgtgc catcgtctcc aatcgtgctt ccccagtgca tgggaacaaa tacgagccca
     2701 ggtcgaattc tttcatccag gcgggctctg agcctgacgc ttcctctttt gctgtgaact
     2761 tctgccagat ccccttccgc aatgccacgc gaaagagcgt cttcggggtt gatctctaca
     2821 tacggttctg ggatatgagt gcccagtttg tttacttttc ccgttcgggt cattgtgtgc
     2881 cattgatctc ggattcttcc agatgtgagc accagcgggt actctgcgct gagtgcttct
     2941 gaacgatttt caaatcgaaa tccatggatc ttcgcgcgac cgctctgtgt gtggaatttc
     3001 aggtcttgaa agaggcgcgg agtgcccgga tgatccggat gaggaacggg ccattgcatg
     3061 ctggattgtt tgagtcgatc gtaggatagt ccggaaacgt ctatgtttgt tcctcgagtg
     3121 agggcgcagt gctccgcgta gatttcttcg gcgcttttgt atgcgaatgc atctgcataa
     3181 cccattctac gtgcaaagtc cccccagatt tcgctatcag gtctcgcttc ccccggcggt
     3241 tcgatgagct ttggaagata cgttatgcgg cgttcagagt tggtcatcgt tccttctttt
     3301 tccagccagc ctgccgcagg gagaacgaga tctgcatatt tgactgtttc tgatctttca
     3361 gaaatatcct gcaccaccac aaatcttgct ttctcaagtc cttcctgcac tgccatggag
     3421 tccggaaggc taacgatcgg atttgtgcat gcaatccaga tcgctttcat ctctccgctt
     3481 cgcagagcat caaacatctc cgtcgcggtt ctgcccggct tcggggaaat gtctttcgaa
     3541 ttccaaaagg ttttcacttc ttcgcgatgt gcctggtttc caatgtcgcg atgagcggcg
     3601 agcatggtgg ccagtccgcc gacttcgcgt ccacccatcg catttggttg acctgtaaga
     3661 gagaacgggc cggatccagg cttgccgatg tgacctgtga tcaaatgaag attgatgaga
     3721 gatagattct tgcgaactcc ctgcgcgctc tggttgagtc ccatggccca gaagctcagg
     3781 aatccgctgg agtctgcaat ccagtccgcg catttctcaa tatcagacgc ggacaggtcg
     3841 catatttccg ccgcttcgcg aacgctgatc gaaagcgccg cttcttcgag agtggaatag
     3901 ttctccgtat gattggaaat gaagttcgag tctatgttcc ctttttctat tagaattcgt
     3961 gcgatcgcat tgtagagtgc gacgtctgtt cccgggcgaa gctgaagatg aagatctgcc
     4021 agggaggccg aatccgtttt tcttggatca atcaagacta cttttcgaac tcgctctccc
     4081 ttctcctcca ttctgcgaaa tagaattgga tgacaccatg ccggattggc tcctgcgatc
     4141 aggtatagat ctgccagttc aatgtcttca tagttgatag gtacgcaatc ttctccgaat
     4201 gccatcttct gtgctgtcac agccgaactc atgcaaaggc gcgaattcgt atctatgttg
     4261 ttcgtcccaa tgaatccttt ggtgagcttg tttacgatgt agtattcctc tgtgagaagc
     4321 tggccggata catagaaacc aacgctgtcc gggccgtatt tttcggtgat ggatctaaaa
     4381 acatttgctg cgcgattgag cgctgtgtcc cacgttacac gactttgaga atgaccacga
     4441 ctcgccctga ggcgcggata gaggatacga tctgttttgt ccgcaattgt gtaaccgaga
     4501 gtgcggccct tggagcagag catcccccgg ttgacaggat gctctggatc accttctact
     4561 tgtaaggtgc ctttgtgctc gcggactaca attccgcagc caacgccgca gtaggaacag
     4621 gtagtccgcg tgccactcag catgggtatg ctgctttctt acgatagtag aagtagtatg
     4681 tcgccgccag acatccaatg tagaatgcga ggaagagaat gagcgctccg gaaggcccgc
     4741 cggtcagttc aatggaagtt ccatagcttt taggtacaaa gaatgctccg tatgctgcga
     4801 tggccgatgt aattccgacc accactccgg actgacggtt tgcgcggttg agactttcgg
     4861 tggttgcggt tgctccttca tgcgcattgt tctttctcat gtagatgata ggaatcatgc
     4921 gatacgtgga gccgtttcca attcccgccg ccacaaagag aatgaggaac atcgcaaaga
     4981 acccgtagaa tgcacctggt tcagtcttga tgctcaagaa atagattacg ccactcactg
     5041 cgaggcccat cactgcgaaa ttccagaagg tgacgcgcgc gcctccaatc ttgtctccca
     5101 gccatccgcc tacgggacga atcacggatc caaccagtgg accaatgaat gcgaactgga
     5161 gaggattgac gttggggaat tgcgatttga tgaggagagg aaatcctgca gaatatccaa
     5221 tgaaagatcc gaatgttccc atgtagagaa gactcatgat ccaggcgttt ttgtctttga
     5281 gcatggtcag ctgttctttc actgaagagc ggctaacgtg tagattgttc atgaagaacc
     5341 atgtggtacc gaacgcgatg acgataaacg gaatccagat gagggctgcg ttttgcagga
     5401 acagagtttt cgtgcctgcc atctggcctg aaccgccaaa agatccaaag
//
LOCUS       DAORFF010000234         5448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8266_length_5448_cov_4.532728, whole genome shotgun sequence.
ACCESSION   DAORFF010000234 DAORFF010000000
VERSION     DAORFF010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8266_length_5448_cov_4.532728"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1052)
                     /locus_tag="PKK76_10355"
     CDS             complement(123..1052)
                     /locus_tag="PKK76_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNL68919.1"
                     /translation="MTGGPVGQIERKLASIIGQDLPVLKKIKKYVIQSGGKRIRPLTH
                     YYTLRMLGSDHKEWVDAGAIGELIHNASLLHDDVVDESDLRRGKPSAPVLFGNKTVVL
                     AGDYLLACGLHHLSTLEHSRELLVIFSRTIRLLSEGELIQMEWEGNLKIPPKAYERII
                     YCKTGCLFGAMTESAAVLAGADSARAQYRAFGERLGRYFQVRDDYLDYFTPAKETGKK
                     EMQDFERGLVTWPVMRLLETKKGADKILRDIWKDRKKNLKKMLGLLEERRIQESSRAY
                     LKSELDSMLGFILKHPPSSFRDKMHSTLKTLEL"
     gene            complement(1049..1918)
                     /locus_tag="PKK76_10360"
     CDS             complement(1049..1918)
                     /locus_tag="PKK76_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNL68920.1"
                     /translation="MSREIESLLLSLYDSSDEKESAYLTKELFRTIPIGYHPLRYVEA
                     LSTPAVRWKLRTGLFPALGDLKIRHDLKELLNRSREDGDMDLEMGAFLIARGVEEPEV
                     VPEDLTVQLDALAKPLARAIKYAGGDPESRLNLFRFYMFDKLGFQGNTADYYDPRNSF
                     ITSLLRTGKGIPVTISVLCILIARRVGLPVSGVNMPGHFLLTFQEGDLVAYMDPFNSG
                     AILSHEECLQFLTRQNLPSDPAYLRIADSMTILKRMYRNLIHIHSSRGNREEERQLRR
                     HLFILENAGAASP"
     gene            complement(1923..3125)
                     /locus_tag="PKK76_10365"
     CDS             complement(1923..3125)
                     /locus_tag="PKK76_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68921.1"
                     /translation="MAALLTLALVWFVPFDASLVKAALVALALLDSFFPVTSRSRMII
                     VSWIVLSQLILAFTGFYFSDDSLRHLHDGFYLLRGIDIYLFPPAALPVVVPTPDYHRM
                     IGSVYFPVVQFMSMIGSLFSVSRGYVFVFHGLCSLLFGILAMRIRGRVLSFFSRFVFS
                     PAFVLIASGRHEDLLAAAIFFLSLTLHSRGAGFFTGLLPFIKPDAAFLSLLTMRKNAA
                     YLLAMIVSASCLLWFSFQFLVISDLTLPAFLSNLRFFADWYVGYNPLSLPGPLFFVRI
                     RPWIALAAFLAGLVFIWRAASSGPSRRFVSILAATGAAGVLFSFALRGVWHPWYFCSA
                     ISALIFFKKRKLAQSIPPYLVLFFLPIADLRAGGGFHFGGFYVAVLGLLVLHLGHKFL
                     TRRNSDRL"
     gene            complement(3128..4171)
                     /locus_tag="PKK76_10370"
     CDS             complement(3128..4171)
                     /locus_tag="PKK76_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010679700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNL68922.1"
                     /translation="MKAAVLEKDARFLQIKEVERPALAAGMVRVKVHACGVCGSDVHL
                     VLHRSMHAKSYPRIPGHESSGVVIEVGENVTRVKPQDRVVVSAGTSCGHCAACKAGRE
                     NLCPEVGVLGFDSDGSFAEEVVVPERCLFVFSSHIPFEQAAILADAVSTPYHALRYAG
                     NIKQGDTVAVFGCGGLGIHGVLLARALGASRVFALDVEAGALSNAEKAGANEIIDLSG
                     KSSVGKMNAGKILKEKGGVDLVVDFSGYYSNIEESVRAMNPGGRMVMVGLGRNPLKFA
                     IPATLIFKMISVCGSYGSDSRALPELIDLLEKGKINLAPSITSTHPLEEAQECIEKLE
                     NRKGNPIRFVIRP"
     gene            complement(4168..4548)
                     /locus_tag="PKK76_10375"
     CDS             complement(4168..4548)
                     /locus_tag="PKK76_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68923.1"
                     /translation="MWMWPALIVSIVLGAIGQILMKIGMKAAGPVPLDRGIVDLFYYF
                     MHAVLSLPLIGAALSYGVSFVLWLGVLSVADLSLARPIMSAGYILTLAYGFASGEDIT
                     PARVLGTCLIVVGIAFVAKSGIRT"
     gene            complement(4593..5075)
                     /locus_tag="PKK76_10380"
     CDS             complement(4593..5075)
                     /locus_tag="PKK76_10380"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL68924.1"
                     /translation="MLLISLEQPGSAAKEGISEAIAYAALESPRLADVIASVHKVAFE
                     KGGILRAGVYELREDEAPGEVPGLFHAVTAGLPPPLLIRGGEFTFPLRSGIPAGIVSE
                     WVSQTQSMEWHAGDALYLYTDSAEVEASHDFHEYLRAHKGGSLQIPEDMIVFRIEFGS
                     "
BASE COUNT         1510 a         1402 c         1342 g         1194 t
ORIGIN      
        1 atacaaacca tcgtcatctg cctccatcaa tccgggcaag gagccacctt cgcgaagagg
       61 caaagcatag cggatggccg taacggtgcg aagtactttg ggcgaagagt ccatgggctg
      121 gctcacaact ccagcgtttt gagggtgctg tgcattttgt cgcgaaaaga agaaggcggg
      181 tgtttcaaaa taaaaccaag catggaatcc agttctgatt tcagataagc tcgcgacgat
      241 tcctggatgc gcctttcttc gagcagaccc agcatctttt tcaaattctt tttacgatct
      301 ttccagatgt cgcgaagaat cttgtcagcg cccttttttg tctccaaaag tcgcataacg
      361 ggccatgtga ccaggcctct ttcaaaatcc tgcatctctt tttttccagt ctcttttgct
      421 ggagtgaagt agtccagata gtcatcgcgc acttgaaaat atcttcccag acgctcgcca
      481 aacgcgcggt actgtgcccg agcagaatcc gcgccggcaa gaacggccgc cgattcagtc
      541 attgctccaa acagacaacc tgttttgcag tagataattc tttcgtatgc ttttggtgga
      601 atttttagat ttccttccca ttccatctga atgagttctc cctcgctgag aaggcgaatc
      661 gtacgagaaa aaattacgag caactcgcgt gaatgttcca gagtggacag atgatgaaga
      721 ccgcacgcaa gcaagtagtc cccggcgaga accactgtct tgtttccaaa aagaacaggt
      781 gcagaaggtt tgccgcggcg aagatccgat tcatccacta catcatcatg aaggagagaa
      841 gcattgtgaa tcagttctcc tatggcgcct gcatccaccc actccttgtg atccgagcca
      901 agcatgcgca gcgtataata gtgagtaagc ggacgaattc gctttccgcc gctttgaatt
      961 acgtatttct taattttctt tagaaccgga aggtcctgcc caatgataga agcaagcttc
     1021 cgttcaatct gaccgaccgg tccgccggtc acggcgaggc tgccccggcg ttctcaagaa
     1081 tgaaaagatg ccggcgcaac tggcgctctt cctcacgatt gccgcgcgac gagtgaatat
     1141 gaatcaagtt acgatacatc cttttgagaa tggtcatgga gtctgcgatt ctcaggtagg
     1201 caggatcaga aggaaggttc tggcgagtga ggaactgaag acattcctcg tggctcagga
     1261 tggctccgga gttaaatgga tccatgtacg ccacgaggtc cccttcttga aacgtcagaa
     1321 gaaagtgtcc gggcatgttg actccggaga caggcaggcc tacgcgacgc gcaatcagaa
     1381 tgcataaaac agaaatcgtc acgggtatcc cttttccggt gcgaagaaga gaagtgatga
     1441 aactgtttct aggatcgtag taatcagctg tgtttccctg aaagcccagc ttatcaaaca
     1501 tatagaatct aaacaaattc aaacggctct ccggatctcc acccgcatac ttgatggccc
     1561 gggcaagagg cttcgcaagc gcgtcgagct gcacagtcag gtcttccggc actacttccg
     1621 gttcctccac tccacgtgcg atgaggaatg ctcccatctc tagatccatg tctccgtctt
     1681 cgcggctgcg gttcagaagc tctttaagat cgtggcgaat cttcagatct ccaagcgccg
     1741 gaaaaaggcc ggtgcgaagt ttccagcgaa ccgcaggagt gctgagtgct tccacgtaac
     1801 gcagtgggtg atagccaata ggaatcgtgc gaaagagttc tttcgtaaga tacgctgact
     1861 ccttttcatc ggaggagtca tagagagaaa gaaggagact ctcgatttcc cggctcataa
     1921 gttcagaggc gatcactgtt tcgacgtgtc aagaatttgt gcccgagatg caataccagc
     1981 aacccgagaa ctgcgacata aaatccgcca aagtgaaagc cccctcccgc acgaagatcg
     2041 gcaattggga ggaagaataa aacgagatag ggcggaatac tttgtgcaag ttttcttttt
     2101 ttgaagaaga tgagcgcgga aattgcagag caaaagtacc atggatgcca gacaccgcga
     2161 agagcaaaac tgaacaggac tccggccgca cccgtggccg ccagaatgga cacgaaacgc
     2221 ctggaagggc cggaagacgc tgcacgccag atgaagacta agcctgccag aaatgcagcc
     2281 agagcaatcc acggacgaat tcgaacaaaa aacagagggc ccggaagact cagaggattg
     2341 tatcccacgt accagtctgc gaagaagcgg agattggata gaaaggccgg aagtgttaga
     2401 tcactgatta ccagaaattg aaaggaaaac cagagcaagc aagaagcact gacaatcata
     2461 gccaggagat aggcggcatt ctttctcatc gtaagcaagg agaggaaggc tgcgtcaggc
     2521 ttgataaagg gaagaagtcc agtgaagaag cctgcacctc gtgagtggag ggtcaaagag
     2581 agaaaaaaaa tcgcggccgc aagcaagtcc tcatgccgac cacttgcaat gagcacaaat
     2641 gctggagaaa aaacaaaacg agagaagaaa gacaacactc ttccacgaat tcgcatcgcc
     2701 aggattccaa ataacagaga acagagtccg tgaaatacaa agacatagcc acgcgataca
     2761 gaaaacaagg agccaatcat agacatgaac tgaacaactg gaaaatagac acttccgatc
     2821 atgcgatggt agtccggtgt gggcacgacc accggcagag ccgcgggagg aaacagatag
     2881 atgtctattc ctcgaagcaa atagaagcca tcgtgaagat ggcggaggct atcgtctgaa
     2941 aagtaaaagc cagtaaacgc tagaatcaac tgtgaaagaa caatccatga aacgatgatc
     3001 attctgctgc gagaggtgac tggaaaaaaa gagtccagga gcgccagtgc gaccagcgcc
     3061 gccttcacaa gagaggcgtc gaatggaaca aaccagacca gtgcaagagt gagtaaggcc
     3121 gccacgttca gggacgaatt acaaatcgga ttggattgcc ttttctgttt tccagcttct
     3181 caatgcactc ctgggcttct tccaatggat gcgtagaagt aatggaaggc gccagattga
     3241 tttttccttt ttcgagaagg tcaatcaact ctggcagagc gcgcgaatcc gatccatacg
     3301 atccacatac actgatcatc ttgaagatga gagtggccgg aattgcaaac ttgagcggat
     3361 ttctgcccag tccgaccatc accattctgc cgccgggatt cattgcgcga acggattcct
     3421 ctatattact atagtatcct gaaaaatcta caaccaggtc cactccgcct ttttctttta
     3481 gaattttgcc ggcgttcatc ttgccaacgc ttgactttcc ggacaaatct atgatttcat
     3541 tggcccctgc tttctctgcg ttagagagag cgcctgcttc tacgtcgagc gcaaacactc
     3601 tagacgcgcc aagagcgcgc gccagtagaa ccccgtgaat gccaaggcca ccacagccaa
     3661 acactgccac agtatctcct tgcttgatgt tccccgcata tcgaagtgca tgatagggag
     3721 tggataccgc gtccgctaaa atggctgctt gctcaaaagg aatgtgagag gaaaaaacaa
     3781 agagacaacg ttctggcacc actacttctt ccgcaaacga tccatccgaa tcgaacccca
     3841 ggactccgac ttccgggcaa agattttcgc gacccgcctt gcaggcagca caatgaccgc
     3901 agctggttcc agcagaaaca accactctgt cctgaggctt caccctggtt acattttctc
     3961 ctacttcgat gactactccg gatgattcgt gaccgggaat tcttggatag gatttggcgt
     4021 gcatgcttct gtgcaacacc agatgaacat ccgatccgca tacaccgcat gcatgcacct
     4081 tcacgcgcac catacccgca gcgagtgctg gcctctctac ttcttttatc tggagaaatc
     4141 gtgcgtcttt ttcgagtacg gcagccttca tgttctaatc ccactttttg ctacaaacgc
     4201 aatgcccact acaatcaggc aagttcctaa aacccgcgcg ggagtgatat cttcacccga
     4261 agcaaagccg taggcaagag tgagaatata gcctgcagac atgataggac gggccagact
     4321 caagtctgct acagaaagaa cacccagcca caacacaaaa ctgactccat aggaaagagc
     4381 cgccccgatc aaaggcaacg agagcaccgc atgcatgaaa tagtaaaaaa gatcaactat
     4441 tccacgatcc agtggaacgg gccccgccgc cttcattcca attttcataa gtatctgtcc
     4501 aattgcaccc aggacaatag agacgatcaa agcaggccac atccacatga acccactcag
     4561 aaggccgtgg tcaaaagatc aaaggtattt tatcaggagc caaattcgat gcgaaatact
     4621 atcatatctt ccggaatttg gagagaaccg cctttgtgcg cgcgaagata ttcatggaaa
     4681 tcgtgggaag cttctacctc tgctgaatct gtgtagaggt acagtgcgtc tcctgcatgc
     4741 cattccatgc tctgagtctg ggagacccat tcagatacaa tcccggcagg aattccagac
     4801 cggagaggga atgtaaactc acccccgcgg atcagcaagg gaggtggaag tcctgcagtc
     4861 acggcatgaa agagtcccgg cacttcccca ggcgcttcgt cttcgcgcag ctcatacact
     4921 ccggcgcgaa ggattccacc tttttcaaac gccactttgt gaacggatgc aatgacgtcc
     4981 gcaagcctgg gcgattcgag tgccgcatac gcgatcgctt cggaaattcc ttcctttgct
     5041 gcagaacccg gttgctcgag ggaaatcaaa agcacgcgaa tgcgttcgtc tgttttcaga
     5101 aagtaagctg cttttgtgta atcgccctga ttgagacaaa gggagagatg ctctttttct
     5161 ccgtttctct ttagctcttg cgccagactc agatgcccgg cgtagacgcg aaaacgaatg
     5221 tccaggatgc ctgcgtggat gctgaaaggc tcaacggttc cgggattcag aggatcactc
     5281 ctttatcgcg aaagattctg tgagagtttt aaacatggat gctagcctcg catcatcatc
     5341 tgacggacca aagactccga ggacgaatat ttttttccca accgcgatgt gcctttcgta
     5401 ggcaatgcgt gatccgtcct tcatggatag aacgtattcg cgtgccgc
//
LOCUS       DAORFF010000235         5429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8315_length_5429_cov_2.998139, whole genome shotgun sequence.
ACCESSION   DAORFF010000235 DAORFF010000000
VERSION     DAORFF010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8315_length_5429_cov_2.998139"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PKK76_10385"
     CDS             complement(<1..575)
                     /locus_tag="PKK76_10385"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNL68925.1"
                     /translation="MSRFEIPREMQALELSANGSTVREACANLKVVRKPVPVPTKGQV
                     LVRVEAAPCNPSDLSYLQGHYAVDRPLPAVPGFEASGVVVKSGGGLIGSWLLGKRITC
                     AGQSPRDGTWAEYFIADAMSCLPLRRGVSFEQGCSLIVNPLTAFALLERAREFGSKAL
                     VQDAAASQLGRMIAALAREQGTASKLGMFFDA"
     gene            complement(664..1812)
                     /locus_tag="PKK76_10390"
     CDS             complement(664..1812)
                     /locus_tag="PKK76_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68926.1"
                     /translation="MKKILTFTFLLLAAASCTPAHPRDTGHWDSVARYLAGMEVQEDP
                     NLAQLSRRPEWVTHKQSMDKMFSGLEENYLSKMKPWAEREIGNPGTPVLFYPFGGPEI
                     IISQTFFPHVREYIMCGLEAPGLPPDPTKVTAARLNPVLTNLRESLKWLTSYTYFQTI
                     HMDSQLRYTDLSGTLPLMLVFLARTGNRVTDVTGVILGNNGTLYELEENSPAPGRIPE
                     SSIKTTGIRGARITFVKNGTERQAYYFSFDASDPAMKTRSYFFDFVKSRGERSVMLKA
                     ASYLLHWGNFPTLKAFILNESRLILSDDTGIPFKDWDRKTWNLTLYGNYQAPIPQFAG
                     QIQYDMLRAYSNKKDVKELPFNIGYTAAWKPGISSLVLARRMDQTEGK"
     gene            2097..2819
                     /locus_tag="PKK76_10395"
     CDS             2097..2819
                     /locus_tag="PKK76_10395"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL68927.1"
                     /translation="MSCVTGLEYVEQDTFGRLSWIIPIAAVMMLADFLFFALVYSSPG
                     GNLITAQVGLASSVIALTLSTALLYLLRTIVDGAILRVEALNLSLSRFVPAEFLRLLN
                     RQSVEQISLGESKEATMSVLFSDIRDFTAIAEGNSPENNFKFINDYLKRMGPVIRDHN
                     GFIDKYIGDGILALFEHADDAVRAGLAMIAELENFNRERSEPVEIGIGIHTGTHHARH
                     NRRRASHGKHCDRRHGQLSIAH"
     gene            2740..3528
                     /locus_tag="PKK76_10400"
     CDS             2740..3528
                     /locus_tag="PKK76_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68928.1"
                     /translation="MLGTIGEELRMENTVIGDTVNSASRIEGLTKVYYTRFLVSRDTL
                     SAMQDHTGIRSRLVDITLVKGKRKPLYVHEVICGEEESIMAARYSGGALLEKGVHAVR
                     SGDYISGKNIFEEYLSQYPFDRTAKVHLQRCARVFNQFKGKSIFDKYGDISTVGSLVE
                     LFYSKILEDDLLSDFFTNTNMVRLKGHQTRFIAMLMGAPIRFDTCALEYAHAHLPITR
                     AHFQRVGLLLENALSEGGMERADIDTIMSRIASFEKVIVSKAES"
     gene            3666..5033
                     /locus_tag="PKK76_10405"
     CDS             3666..5033
                     /locus_tag="PKK76_10405"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL68929.1"
                     /translation="MSWIQKIRNLLLLGDLLRGLGTEEARAVRVTNWIQIIFLTITTL
                     LWPQVLINEPPETHAVSSLLYVQIVLFMVIGRIFLYARRFYVSSIITLAGVFIHLGGL
                     CVAYADDPAHYGYFLLMLLPFLLIPSRHFWTRWAFVVIAGLGFLADQVYFRIMSGAAL
                     FGPPKSVLPADYLMPPMIMVVIFYALLINSFVKAVTTAETALAKEHAASERLLLNILP
                     EEIAAELKVKGASDPRYFEATTICFTDFAGFTQIAESMTPTDLVAELDKCFSYFDRVI
                     GRNGLEKLKTIGDSYMFAGGVPVPSPTHAIDSVMAALEIQAFMNQMKEIKSQQGLPYW
                     ELRLGIHSGDLVAGVIGERKFAYDVWSDTVNTASRCESSGVSGKINISKATYELVKDF
                     FQCEYRGPIPAKNKGTIDMYFVNGILPGLSRENEPRVPNQAFRDLYQRKVEEGGKAAG
                     KAAAG"
     gene            complement(5040..5112)
                     /locus_tag="PKK76_10410"
     tRNA            complement(5040..5112)
                     /locus_tag="PKK76_10410"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5077..5079),aa:Asn,seq:gtt)
     gene            complement(5198..>5429)
                     /locus_tag="PKK76_10415"
     CDS             complement(5198..>5429)
                     /locus_tag="PKK76_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68930.1"
                     /translation="SLVSALGFHPFSLGPAFLILGFAWVVAATLLLLKPAVGRILSLC
                     LAVVTLFYLPVGTLYAVCVIALLFRPETPAQD"
BASE COUNT         1307 a         1339 c         1447 g         1336 t
ORIGIN      
        1 gcatcaaaaa acatgccgag tttggaagcg gtaccttgct cgcgggcaag tgctgcaatc
       61 attctgccca gctggcttgc ggctgcatcc tgaacgagag ccttgctccc gaattctctc
      121 gcacgttcga ggagagcgaa tgcagtgaga gggttcacaa tcagcgaaca accttgctca
      181 aagctcacgc cacggcgaag cggaagacag gacattgcat ctgcaatgaa gtattctgcc
      241 catgtgccat cgcgtggact ctggccggcg cacgttatgc gctttcccag gagccaggat
      301 ccaataagcc caccgcccga tttcacaacc acaccggaag cttcgaagcc aggtaccgcg
      361 ggcaggggcc ggtctactgc gtaatgaccc tggagatagg agagatcgga cggattgcac
      421 ggcgcggctt ccactcgcac aagaacctgt cctttcgttg gaactggcac tggcttgcgc
      481 acaactttca aattggcaca agcttcgcgc acagtggatc cgtttgcgct cagttccagc
      541 gcttgcatct cgcgtggtat ttcgaaacga ctcatctgct gcctccctat tccacaccgc
      601 gccagcgatc gacggccgcg ccctgcggcc cgagagcgcg gtccgcgaag cggaggcgcc
      661 caatcacttc ccctctgtct gatccattct tcttgcgagc accaggctcg aaatgccagg
      721 cttccaggcg gccgtgtagc caatgttaaa tggtagttcc tttacgtctt tcttgtttga
      781 atacgcacga agcatatcat actggatttg tcctgcaaac tgaggaatgg gtgcctgata
      841 atttccgtag agcgtgagat tccaggtttt gcgatcccaa tctttgaacg ggattcctgt
      901 atcgtcggat agaatgaggc gcgattcatt caggataaaa gccttgagag ttggaaaatt
      961 gccccagtga agaaggtagg acgcggcttt cagcatgact gacctttcac ccctgctttt
     1021 cacaaaatca aaaaagtagg atctcgtttt catagccggg tctgaggcat cgaatgagaa
     1081 gtagtaggcc tgtctttccg ttccgttctt tacgaacgtt atgcgcgcac cgcgaatgcc
     1141 cgttgtcttt atggaggatt ccggaatccg accgggtgca ggcgaatttt cttccagttc
     1201 gtagagtgtt ccattgtttc caagtatgac accggtgacg tccgttacgc gatttcctgt
     1261 tcgcgcaaga aacacaagca tgagtggcag ggttcccgaa aggtccgtgt atctcagctg
     1321 gctgtccatg tgaatcgtct gaaagtatgt atagctcgtc agccatttga gcgactcacg
     1381 aagattggtg agaacaggat tcaaccgggc agccgttact ttcgttggat cgggtggaag
     1441 gcccggcgct tccaggccgc acatgatgta ttcacgcaca tgaggaaaaa aggtttgaga
     1501 tatgatgatc tccggtccac cgaacggata aaatagaact ggcgttcccg gattgccgat
     1561 ctctcgctct gcccagggct tcatcttcga aagatagttc tcttccaggc cagagaacat
     1621 tttgtccatg gattgtttgt gagtgaccca ttccggtctg cgagatagct gagcgaggtt
     1681 cgggtcttcc tggacttcca tgcccgcaag atatcgcgcc acagaatccc agtggccagt
     1741 atcgcgtgga tgggcgggcg tgcaggaggc ggccgcaaga aggagaaagg taaaggttag
     1801 aatttttttc ataggtgatc tgttcagaag cgaacattcg tggagtattt gcacctggtt
     1861 tgcggcactt ctattcttcg cacacaaaaa aaatgggccc gaaacgccgg cttttttttg
     1921 aggaatggcg cgccagcggc caatgtgatc tggcacccag aacgactccc gtttagctcc
     1981 aacacttgtg ggccgcttcc atcgtttgtg aagcttcgcg ttgtaaggcg aaccattgtg
     2041 actgcacagt taatgcattt acagaaaagc agagagtcga tttttcattc cagcacatgt
     2101 catgtgtgac cggattggag tatgtggaac aggatacatt cggacgcctt tcctggatca
     2161 tccccattgc agcagtcatg atgctcgcgg actttctgtt ctttgctctc gtatactcca
     2221 gtcccggcgg gaatctaatc acggcacagg tgggtcttgc atcttctgtg atcgctctga
     2281 ctctgagcac tgcacttctc tatcttttgc gaaccatagt ggacggggcg atcctcagag
     2341 tggaagccct gaatctttct ctttctcgat ttgttccagc tgaattcttg cgtttgttga
     2401 atcgccagag tgtggagcaa atttcactcg gagaaagcaa agaagccacg atgagtgttc
     2461 tgttctctga cattcgcgat tttactgcaa tcgcggaagg gaactctcca gagaacaatt
     2521 tcaaattcat aaacgattat ctgaaacgaa tgggacccgt catacgcgat cacaatgggt
     2581 tcattgataa atacattgga gacggaattc tggccttgtt cgaacatgca gacgacgcag
     2641 tgcgggcagg gctcgctatg atcgcggaac tagaaaattt caatcgtgag cgatcagaac
     2701 cggtggaaat cggaatcggc attcacacag ggacgcatca tgctcggcac aatcggagaa
     2761 gagcttcgca tggaaaacac tgtgatcgga gacacggtca actcagcatc gcgcattgag
     2821 ggacttacaa aggtgtatta cacaaggttt ctcgtgagcc gggacactct gagtgcaatg
     2881 caggatcaca cagggattcg ttcacgcctg gtggacatca ctcttgtgaa aggaaagcgc
     2941 aagcctttat acgtacacga agtgatctgc ggagaagagg agtccatcat ggctgcgaga
     3001 tactcaggcg gggcgttgct tgaaaaggga gtgcatgcag ttcgatccgg agattacatt
     3061 tctggaaaga atatcttcga agagtatctt tcgcaatatc catttgaccg cactgcgaaa
     3121 gttcatttac agagatgcgc tcgcgtattc aatcaattca aaggaaaatc gatctttgat
     3181 aagtatggag atatttctac tgtaggaagt ctggtggagc tcttctactc taagatctta
     3241 gaggatgatt tgctcagcga ctttttcacg aacacgaaca tggtgcgtct gaagggacac
     3301 cagactcgat tcatcgccat gcttatgggc gcacccattc gatttgacac ttgcgctctg
     3361 gaatacgcgc acgctcattt gcccatcacg agagctcatt ttcagagggt gggcctcctt
     3421 ctcgagaacg cactttctga aggaggaatg gagagggcgg acattgatac aatcatgtcg
     3481 cgcattgcaa gttttgaaaa ggtgatcgtt tcaaaagcgg agtcctgaca gcttgctaac
     3541 catggcgacc tcaatgagta taacagcagt tttgcgattg tttgcgcaaa acaggattga
     3601 gcctcgcgcg cgcaggcgcc cataaatgtg attgcctcgc ttttttcata gaggcgaatg
     3661 cacttatgtc ttggattcaa aagattcgca acttattgct gttaggggat ctactccggg
     3721 ggcttggcac agaggaagcg cgcgcggttc gcgtcactaa ctggattcaa atcatcttcc
     3781 ttacaatcac tactctgttg tggcctcagg ttcttataaa tgaacctccg gagactcacg
     3841 cagtgagttc tcttttgtat gttcagatcg tcttgttcat ggtgattggt cggatttttc
     3901 tctatgcacg ccgattttat gtgagctcca tcatcactct tgcaggcgta ttcatccatc
     3961 tagggggact ttgcgtggcc tatgcggatg atccagcgca ctatggatat tttcttttga
     4021 tgctgctgcc gtttttgctg atcccatccc ggcatttttg gacgcgctgg gcttttgttg
     4081 tgatcgcggg tctcgggttc ctggcggatc aggtctactt tcggatcatg agcggcgcgg
     4141 ctctctttgg cccgcctaaa tccgtgctcc cggcggacta tctcatgcct cccatgatca
     4201 tggttgtaat tttctacgcg ctgctcatca attcgtttgt caaagcagtc accacggcag
     4261 agaccgctct ggcaaaagaa catgctgctt ccgaaagact cttgctcaat attttgccgg
     4321 aggaaattgc cgcagaattg aaagtgaaag gagcaagcga tcccagatat tttgaagcga
     4381 ccaccatctg cttcaccgat ttcgcgggct tcactcaaat cgcagaatcg atgacaccaa
     4441 ccgatctggt ggcggagctc gataaatgtt tttcctattt cgatagagtg atcggacgca
     4501 atggactgga aaaattgaaa acgataggcg atagctacat gttcgccggc ggagttcccg
     4561 ttccttcgcc gacgcatgcg atcgactcgg ttatggctgc tctcgaaatc caggcattca
     4621 tgaatcagat gaaggaaatc aaaagccagc aaggtcttcc ttactgggaa ctgcggcttg
     4681 gaattcattc gggagatctg gtggcaggtg tgattggaga acgaaaattt gcgtacgatg
     4741 tatggagcga cactgtaaac actgcgagtc gctgcgaatc ctccggagtt tcgggtaaga
     4801 tcaatatttc gaaagcgacc tatgagctcg taaaagattt ttttcagtgc gaatatcgcg
     4861 ggccgattcc ggcgaaaaac aaaggaacga tcgacatgta tttcgtaaac gggattcttc
     4921 cagggctttc gcgcgagaat gaacctagag tgcccaacca ggcgtttcgg gatctttatc
     4981 agagaaaggt ggaggaaggg gggaaggcag ccggaaaagc tgcggctggt tgaagaacgc
     5041 tcccccgcct gggctcgaac cagggaccta gtgattaaca gtcacgcgct ctaccgactg
     5101 agctacaggg gattgttgcg gccatgttcg atcgcaggcg cctccgggtc aattacaaat
     5161 gcctgggacc ccggcttccg cgccagattc cggggagtta gtcctgcgcg ggtgtttccg
     5221 ggcgaaagag aagcgcaatc acacagaccg cgtagagagt cccgactggc agataaaaaa
     5281 gagtcaccac ggccagacag agagaaagga ttctgcccac cgccggtttc aaaaggagca
     5341 gagtcgcagc caccacccat gcgaatccga ggatcaaaaa cgcaggaccc aggctaaagg
     5401 gatggaaacc cagggcagag acaaggctt
//
LOCUS       DAORFF010000236         5421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8327_length_5421_cov_3.947820, whole genome shotgun sequence.
ACCESSION   DAORFF010000236 DAORFF010000000
VERSION     DAORFF010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8327_length_5421_cov_3.947820"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PKK76_10420"
     CDS             <1..369
                     /locus_tag="PKK76_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68931.1"
                     /translation="RTYVESPDQKTESCLYAALYAKMPSSLVNIAMIMPGGNLTSILR
                     FSNDAHDGLILSSIPEAEPDTGIYFALFGRLALRLPMDESFHLVADNEHIRALHSMEL
                     FGYTFLTLDFEIAKANLRSA"
     gene            366..2024
                     /locus_tag="PKK76_10425"
     CDS             366..2024
                     /locus_tag="PKK76_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNL68932.1"
                     /translation="MTRSSSTPFSREMSSFTATKWRFRLALGFLIFAAAAAWAASYYH
                     IAYIFSDSARGNEDVAKLLTLVDILSGSLLAALLFAAVFIFRPLVLGLGRQHRILESL
                     ESRSRAVVESAPDGILAFDEKGIIESFNPAAEKLFACSASEVIGKSIQTIIPGYRGSL
                     EVNAETSASRITGIERQIVGHRWDGSTFDMDLTVSELMVDDKKLTMAIIRDVSARTEA
                     EQQMKESEERLRLIVEHSPDAIAIHAAGTVIFVNPRGVELLGAQKAGDILGRPILDFV
                     PHEMRKTVIERIQTMIQKNEKVPMLEERFVRLDGKAVDVEVIAIPLSVGGKPAIQVVA
                     RDISRRKAADAEIRKSLKEKEVLLKEIHHRVKNNLQVIVSLLDLQSDRVEDRVALEAL
                     RECQNRVKSISLIHERLYESRDLAQVDFPEYIRMLMDNLLRSYGVEPDRVRMRIEVVD
                     IRLGLDTAIPCGLIIHELVSNALKHAFPGGRSGEIRVGIYADGDRNFLVVSDNGKGFG
                     GVSDFRTVSSLGLQLVDTLTSQINGNIDFHTNGGTEFIISFQQA"
     gene            2034..3215
                     /locus_tag="PKK76_10430"
     CDS             2034..3215
                     /locus_tag="PKK76_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL68933.1"
                     /translation="MKEAKILIVEDENIVALNLQTRLKSLGYQVAGMASTGEDAVKKC
                     AETMPDLVLMDIMLRGEMDGVAAADQIREKYHVPVVYLTAYADDVTLERAKVTEPFGY
                     MLKPFEVKEIRTTIEIALYKHRMDRLLQESEERYSVAVHGSNDGIWDWDLVKNQVYYS
                     PRWKAILGCTDAEIGNTPEEWLSRVHPEEREALDMAIERHLAAVTPHMECEFRILAKN
                     GQHRWVFARGLAVRGTGGKAIRMAGSLADITRRHQAEEKLVRMTEHDPITDLLTESAF
                     LEKLYHAAGGSRDSTVMRLSLTKFNSVYAAQGPASAHDLAAHAAKVMISRLTSGAIAR
                     LFGGEFAILLADADPVRVKLLLTEITQDLDRLSIPCVTGFAILKAGGDYNLALCEAAK
                     Y"
     gene            complement(3240..4100)
                     /locus_tag="PKK76_10435"
     CDS             complement(3240..4100)
                     /locus_tag="PKK76_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL68934.1"
                     /translation="MKRCLVVPIFLLTACFPYFKQIEKDKQTFLDRFPNAHMYYESGG
                     RRMHYVRAGQGRDLIVFVHGSPGSWDAFARFLMNESLLSRARVISFDRPGFGESGNGT
                     AELSLERQAALIAEGVRLEQSGPAVRPGRIQPVSTGRVILVGHSFGGPVIARMAMDRP
                     LSYHALVFVAASVDPDLETVHWYQKAGNTGVVRFFLPAMLDVTNQEILPLRSELLAMI
                     PLWQNIKSRSYVIQGGRDDLVPPGNADFLEKNLRQVPLTVVRDPAMDHFVPWAHPDLI
                     EAALLDALAH"
     gene            complement(4121..4603)
                     /locus_tag="PKK76_10440"
     CDS             complement(4121..4603)
                     /locus_tag="PKK76_10440"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNL68935.1"
                     /translation="MLEAVRTFWKYNFWAQDQFFAVVSELTDEEFTRDLGDGNGSIRD
                     KLGHVCAADDVWMRRIEGEVSPPFKLDFTDKFNYIERQKAVRHKYHALIDGLSEVDLL
                     REISYKNLKGVEFTTPLVQILLHVSNHATYHRGQAASLIRRVKGKPPVTDMIEYFRAH
                     "
     gene            complement(4605..>5421)
                     /locus_tag="PKK76_10445"
     CDS             complement(4605..>5421)
                     /locus_tag="PKK76_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNL68936.1"
                     /translation="AQYLQKTLLRQVILCNDRPGFAGNRIGFQLMNEAAQMAEGVQDK
                     GGIALLDAILGGYTGRAMSPLATVDLVGLDVHKAIVDNIYANTKDNAHETFKLPAYMQ
                     KLIDAGSLGNKAGKGLYRREKKADGTTDRFVYNIKKGDYEPLAKIQVPFKKDAVTAIQ
                     ASDYQKLGSIILTAPGMEADLLRHFIARYIAYSFSLIPDVTDANGVDGAMGFGFNWVP
                     PTAWVDVLGGVDAVRKFIEKEKIQVPEFLAKASGSPFFSLRGKLEYRQLFRAG"
BASE COUNT         1418 a         1377 c         1365 g         1261 t
ORIGIN      
        1 agaacgtatg ttgaatcgcc agatcaaaaa acagaatcat gtctttatgc ggcactctac
       61 gcgaagatgc cgtcctctct tgtaaacatc gccatgatca tgcccggagg caatctcacc
      121 agcatactgc gcttttccaa tgacgcgcac gacggactca tcctgtcttc tatccccgaa
      181 gctgaaccgg acacaggcat ctatttcgct ttgttcgggc gccttgccct tcgcctgccc
      241 atggacgaat cgttccacct ggtggcggac aacgagcaca ttcgggcact gcattccatg
      301 gaactattcg ggtacacatt cctgactctg gattttgaga tcgcaaaagc aaatctcaga
      361 tccgcttgac gcgcagctct tctactccat tctccaggga gatgtcttct ttcactgcaa
      421 ccaaatggcg atttcgcctc gccctgggat ttctcatctt tgctgccgca gccgcatggg
      481 ccgccagcta ttatcacata gcgtacatct tctctgattc agcacgaggc aatgaagacg
      541 ttgcaaaact tctcaccctc gttgatattc tttctggatc ccttctcgca gcacttttgt
      601 tcgcggccgt ttttatcttt cgcccactgg ttcttggcct gggccgtcag catagaattc
      661 tggaaagtct ggaatcaagg tccagagctg tggtagagag tgcaccggac ggaattctgg
      721 cattcgacga aaaagggatc atcgaatctt tcaatcctgc ggcagagaaa ttgtttgcat
      781 gcagtgcatc agaagtgatc ggaaaaagca tccagaccat catcccgggt tacagaggct
      841 ccctcgaagt aaatgcggaa acatctgcat cacgcatcac cggcatagaa aggcaaatcg
      901 tgggacacag atgggatgga tccacatttg acatggatct taccgtaagt gaactcatgg
      961 tggacgacaa aaaactaacc atggcgatca tccgtgatgt atctgcgcgc acagaagcag
     1021 aacaacaaat gaaagaaagc gaagaacgct tgcgtttgat cgtggaacat tctccagatg
     1081 ccattgcgat tcacgcagcg ggcacggtga tttttgtaaa cccgcgagga gtggagcttc
     1141 tgggagcaca aaaggccgga gacattctgg gtagacccat actcgatttt gttccgcacg
     1201 aaatgcgaaa aacagtcata gaacggatcc agaccatgat tcagaaaaac gaaaaagttc
     1261 ccatgcttga agaaagattc gtgcgtctgg acggcaaggc ggtcgacgta gaagtcattg
     1321 caattccatt gtctgtcgga ggtaaaccgg ccattcaggt ggtcgcgcga gatatctcac
     1381 gcagaaaggc ggcggatgca gaaatcagaa aatcgctcaa agaaaaggaa gttcttctca
     1441 aagaaattca tcatcgcgtg aaaaacaatc ttcaggtcat tgtcagcctc ctcgacctgc
     1501 agagcgatcg agtggaagac agagtggcac tcgaagcatt gcgcgaatgt cagaatagag
     1561 tgaaatccat ttccctgatc catgaaagac tctacgaatc tagagatctg gctcaggtag
     1621 attttccaga gtacattcgc atgctcatgg acaatttact gcggtcctac ggtgtggaac
     1681 cggacagagt gcgcatgcga atcgaagtgg tggacattcg cctgggactc gacactgcca
     1741 tcccatgcgg tctgatcatc catgaacttg tgtccaacgc gctcaagcat gctttcccgg
     1801 gcggacgttc aggagaaatc agagtaggca tctacgcaga cggtgataga aatttcctag
     1861 tggtgtcaga caatggcaaa ggattcggag gggtcagcga ttttagaact gtatcctcgc
     1921 tgggtcttca acttgtggat acattgactt cgcaaatcaa cggaaatatc gatttccata
     1981 caaacggtgg tacagagttt atcatttcat tccagcaggc gtaaggagcg actatgaaag
     2041 aggcaaagat cttaatcgtc gaagacgaaa acattgttgc gttgaacctg caaaccaggc
     2101 tcaaaagtct gggctatcag gtggcaggca tggcctcaac cggggaagac gcggtgaaga
     2161 agtgcgcaga aaccatgcct gatctagtgc tcatggacat catgttacgc ggagaaatgg
     2221 acggagtagc cgcagcagac cagatccgcg agaagtacca cgtgcctgta gtctatctca
     2281 cagcatatgc ggatgatgtg actctagagc gtgcgaaggt aacagaaccg tttggttaca
     2341 tgctcaagcc cttcgaagta aaagaaattc gcacaaccat tgagatcgcc ctgtataaac
     2401 accggatgga tcgcctgctt caggaaagtg aagaaaggta ctccgttgca gttcatggct
     2461 caaacgacgg aatctgggac tgggatcttg taaagaatca agtgtactac tcccccagat
     2521 ggaaggccat cctgggatgc acggacgcag aaatcggtaa cactccagag gagtggctgt
     2581 cgcgcgttca tccggaggaa cgcgaagcac tggacatggc catcgaaagg catctggccg
     2641 ccgtgactcc gcatatggaa tgcgagttta gaatcctcgc aaaaaacgga cagcatcgct
     2701 gggtcttcgc acgcggtctg gctgtgcgcg gcaccggagg aaaggcaatc agaatggcag
     2761 gatccctggc tgacattacg cggcgccatc aggcagaaga aaaactggtt cgaatgacgg
     2821 aacacgatcc tatcacggac cttctgactg aatcagcgtt tcttgaaaaa ctttaccatg
     2881 ctgccggcgg aagtcgggac tctaccgtta tgcgcctttc attgacaaaa ttcaattctg
     2941 tttatgcagc ccaggggcct gcatctgcac atgacctggc agcacatgct gcaaaagtga
     3001 tgatctcaag attgacgtca ggcgccattg cgaggctatt cggcggagag tttgccatac
     3061 ttctggcaga tgcggatcca gtgagggtga aattattgct cactgaaatc actcaggacc
     3121 tggatcggct ttctattcca tgtgtgaccg gctttgccat tctcaaagca ggtggagatt
     3181 acaatctggc gctgtgcgaa gctgcaaagt actgaagaat atcaatggca tcccttagct
     3241 caatgagcca gtgcatccag gagtgcagcc tcaatgagat ccggatgggc ccagggaaca
     3301 aagtggtcca tggcaggatc gcgcacaacg gtcaggggca cctgacgcaa attcttctcc
     3361 aggaaatctg catttccagg tggaacgagg tcatcgcggc ccccctgaat tacgtaagac
     3421 ctggatttaa tgttctgcca cagaggaatc atcgcaagta gctcggaccg gagaggaagt
     3481 atttcctgat tggttacgtc cagcatcgca ggcaggaaaa atcgaaccac gcccgtattt
     3541 cctgccttct gataccagtg gactgtttca agatccggat ccacggaagc ggcaacaaat
     3601 acgagagcat gatacgacag ggggcgatcc atagccatgc gcgcaatgac aggtccacca
     3661 aatgaatggc ctaccaatat gacgcgtcca gtactgactg gttgaattct tccaggacga
     3721 actgctggac cactctgctc aagacgcaca ccttctgcga tcaatgccgc ctgacgttcc
     3781 agactcaact cagcggttcc gtttccggat tcgccaaacc ctggacgatc aaatgaaatc
     3841 actctcgcgc gcgaaagtag agattcgttc atcaaaaagc gtgcaaatgc atcccaggag
     3901 ccaggagagc catgaacgaa tacgatgaga tcccggccct ggcctgcgcg cacatagtgc
     3961 atgcgcctgc cgccggattc gtagtacatg tgcgcatttg gaaatcggtc gagaaacgtc
     4021 tgtttatctt tttctatctg cttgaaatac ggaaaacagg ctgtcagaag gaaaatggga
     4081 acaaccagac atcttttcac gctgattgct cctcaagcgg ctagtgcgct ctaaaatatt
     4141 cgatcatgtc cgttaccgga ggtttgcctt tgacccgccg aatgagggac gctgcctgcc
     4201 cacgatgata tgtagcgtga ttggaaacgt gaagcaaaat ttgaacgagc ggagtggtga
     4261 attccactcc tttgaggttc ttgtaagaaa tttcacggag caaatccact tctgaaagcc
     4321 cgtcgatcag cgcatgatac ttgtgccgaa cggctttctg gcgttcaatg tagttgaatt
     4381 tatccgtaaa atcgagtttg aatggagggg acacttcccc ctcaattctg cgcatccaga
     4441 catcatctgc agcacacaca tgtccgagtt tgtctcgaat ggatccgttt ccgtctccga
     4501 gatctcgcgt gaattcttcg tccgtcaatt cagagacgac tgcgaagaac tggtcttgag
     4561 cccagaaatt gtacttccag aatgtccgga ccgcttcgag catcttaacc cgcccggaac
     4621 agctgacgat actccagttt gccgcgcagg ctgaagaaag gagaaccgga tgcttttgcc
     4681 agaaattctg gaacctgaat cttttctttt tcgatgaact tgcgcaccgc gtccactccg
     4741 ccgagcacgt ctacccatgc agtaggtgga acccagttga aaccgaatcc catcgcacca
     4801 tctactccgt ttgcatctgt cacatctgga atcaatgaga aagagtaagc aatgtaacga
     4861 gcaatgaagt gtctcaacag atccgcttcc ataccaggtg cagtcagaat gatagaaccc
     4921 agcttctgat aatcggaagc ctgaatggcg gtaaccgcat cctttttgaa tggaacctgg
     4981 attttggcca gaggctcata gtcgcctttc ttgatgttgt atacaaagcg atctgtggtt
     5041 ccgtccgctt tcttctcacg gcgatacaaa ccttttcctg ccttgtttcc caatgaaccc
     5101 gcatcgatca gcttttgcat gtaggcagga agcttgaatg tttcgtgcgc attgtctttt
     5161 gtattggcgt agatgttatc cacgattgcc ttgtgaacgt ccagacccac cagatccaca
     5221 gttgcaagcg ggctcatggc gcggccagta tacccgccca ggattgcgtc cagaagagca
     5281 attccgcctt tgtcctgaac cccttccgcc atttgcgcgg cttcgttcat gagctggaat
     5341 ccaattctgt ttcctgcaaa tccgggtctg tcattgcaca gaatcacctg gcgaagaaga
     5401 gtcttctgaa gatactgagc g
//
LOCUS       DAORFF010000237         5357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8495_length_5357_cov_3.360430, whole genome shotgun sequence.
ACCESSION   DAORFF010000237 DAORFF010000000
VERSION     DAORFF010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8495_length_5357_cov_3.360430"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKK76_10450"
     CDS             complement(<1..592)
                     /locus_tag="PKK76_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68937.1"
                     /translation="MWTQKITRSHRIMVLICAVLMSIVYLVPLWRIHLGAPQYPEGLS
                     MYIWTSNISGGDEFDLQNINLLNHYIGMHEIAPDAIPELKYMPYVLGFMIFGAIMTVI
                     IPRLFMVLLGIINTILVAIVGLADFWHWEYVYGHNLKPDAAIQVPGMTYQPPLLACKE
                     MLNITACSWPHGGVALIVLSMAILVYVVYEERKNRTV"
     gene            complement(685..2577)
                     /gene="nosZ"
                     /locus_tag="PKK76_10455"
     CDS             complement(685..2577)
                     /gene="nosZ"
                     /locus_tag="PKK76_10455"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HNL68938.1"
                     /translation="MKRLQIISSVFFALVAFVACGKAGTAGLASDAAERVYVKPGDKD
                     EIYMFASGGFSGQVGVNGIPSGRLIRVIPVFSQWPETGYGYDETTKNMLMTSHGFVPW
                     DDSHHPQLSVTEGKQDGRWLFINGNNTPRIARIDLKSFETVEIIELPNSAGNHPSSFL
                     TENSEYALAGTRFSIPIPNVPMAWEQFQKEMKAGMSFVSIDKTTGKMSIDFQVLLPGI
                     SYDLGRCGKGPSHDWCFISSYNTEQAYKMLEVNASKKDKDFVMAINWKKAKACVDQGK
                     ARMFPGAYRHNYREENQEAVSESLNGTKMLDPKDCPGMVFYMPTPKSPHGSDVDPTGE
                     YIVAGGKLATVIPVHSFSKMLKAIEDKAFSGEFFGIPVLKYEATLAGEVQKPCLGPLH
                     TEFDGKGYAYTSCFVSSEAVKWKLGTWEVVQHLPAYYSIGHLSIVGGDTRDPFGKYLV
                     LLNKITKDRYLQTGMELVQSAQLYDISGSNAELLLDFPTHGEPHYAQMIPASALKKQT
                     KILRLEDNKNPRATKKESDARVVNEGGAVHVYMTMIRSHFKPDVVEAAAGQTVYFHLT
                     NLEQDFDVPHGFAVFGSNAPNVLVMPGETRTIKYEAKDRGIFPFYCTDFCSALHQEMQ
                     QYIRVQ"
     gene            complement(2588..3019)
                     /locus_tag="PKK76_10460"
     CDS             complement(2588..3019)
                     /locus_tag="PKK76_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNL68939.1"
                     /translation="MKIGTALVTALFLAVALAACKKQSGDNASNDSGTGIGPVSSVQI
                     AAGIDEGMAKKGADLFEAKCSACHKWEEKVVGPALQGVTTRRKPEWIMNMILNPVEMT
                     QKDPTAQKLLEEHLTQMTFQNVTQEEARMILEHFRKKDGAK"
     gene            complement(3227..3754)
                     /locus_tag="PKK76_10465"
     CDS             complement(3227..3754)
                     /locus_tag="PKK76_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNL68940.1"
                     /translation="MRLQWITLLLSVALFATACKDKSGPAGGGIANVSDEESAKTVLQ
                     IAAGSKDHTTLVKAVQAAGLENSLANQGPFTVFAPTDAAFKALPSGALDKVMADKNLL
                     TTVLGYHVYVGSSLKESMLTDGDELSMFDGGTAKISVKGGKISINGANVLAAVPASNG
                     MVYVIDKVLLPPEKK"
     gene            3946..4470
                     /locus_tag="PKK76_10470"
     CDS             3946..4470
                     /locus_tag="PKK76_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNL68941.1"
                     /translation="MAQCSHDRFAISGSPVKGPAGARALFEFTDFDCAPCREVQETLR
                     RAQAAQPVRLVFKSLPLADTEAGRLPHLVAFCVHAQNKDAFWLYYDLMFSSSAPARDK
                     DFLTTVSRTLDLDEEAMKKCVGSEEAASALKKDRADAQAAGVAGTPTFFLGNARLEGN
                     KSTAEFLAFVKANR"
     gene            complement(4556..>5357)
                     /locus_tag="PKK76_10475"
     CDS             complement(4556..>5357)
                     /locus_tag="PKK76_10475"
                     /inference="COORDINATES: protein motif:HMM:NF015252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HNL68942.1"
                     /translation="VQKLKTGDVNFVWEVWAMRAKCAEETSAQDCDASILSYIEKHYS
                     SPEKEKMLSLFKKYFQYEAEMRDTDLGANSSFNEKYDTMKKKRRDILGKEDAELIFGM
                     EEAQVDFIGLSQRQINASKNLSGDERVRQYEALKKQTFGQYYDAEIKREDKFQNYQTE
                     LTLRENDLAKLSDADRAKRTQAIQEQYFGKDAAAAIAKAQEEEAAEQKKLADFEQKQK
                     DFLAQNAAMPAAEKAEKLKAIRIQILGEEEAAAYERRKAFEDAVSKIK"
BASE COUNT         1280 a         1361 c         1248 g         1468 t
ORIGIN      
        1 aaacagtacg gttcttgcgt tcctcataca ctacataaac cagaatggcc atggaaagga
       61 caatcaaagc aacgccgcca tgaggccaac tgcaggctgt gatgttcaac atttccttgc
      121 acgcaagcaa aggaggctgg taggtcatcc ctggaacctg aatggcagcg tccggtttta
      181 gattgtgacc gtatacatac tcccagtgcc agaaatcggc cagtcctaca atcgccacta
      241 gaatcgtatt gatgatcccg agcaggacca tgaagagccg cggaatgatc acagtcatga
      301 tcgcaccaaa aatcatgaat ccaagaacat acggcatata cttcagttct ggaattgcat
      361 ccggagcgat ctcgtgcatc cctatgtagt gatttagcaa gttgatattc tgaagatcaa
      421 actcatctcc gccggaaata ttcgaagtcc agatgtacat ggaaagtcct tcggggtact
      481 gaggtgcacc caggtgaatt ctccacagag gaaccagata tacaatgctc atgagcactg
      541 cgcatattaa gaccataatt ctatgagagc gtgttatctt ttgtgtccac atggtagtcc
      601 tgccctgcac gtttccgtga ctcacacaaa tattagaaac ggggccgaaa aagaggtgcc
      661 cgggggatta ccccgggcac cggattattg aacccgaatg tactgctgca tttcctgatg
      721 aagcgcagaa cagaagtctg tgcaatagaa cgggaagatt ccgcgatctt ttgcttcgta
      781 cttgatggtc cgtgtttcac ctggcataac cagaacgtta ggcgcatttg aaccaaacac
      841 tgcgaaacca tgtggaacgt cgaagtcttg ttcgaggttc gttaaatgga aatacactgt
      901 ctgacccgct gctgcttcca ctacatctgg cttaaagtga gagcgaatca tggtcatgta
      961 aacatgaact gctccgcctt cgttgacgac gcgcgcatca gactctttct tggttgctct
     1021 tggattctta ttatcctcga ggcgaagaat cttggtctgc tttttgagcg cggacgctgg
     1081 aatcatttga gcatagtgcg gctctccatg agtcgggaag tcgagcagaa gttctgcatt
     1141 cgatcctgag atatcataga gctgagcact ctgtaccagt tccatacctg tctgaaggta
     1201 gcgatccttt gtgatcttat tcaacagaac cagatatttt ccgaacggat ctcttgtgtc
     1261 accacctacg atcgaaaggt gaccaatgct atagtatgct ggaaggtgct gaaccacttc
     1321 ccatgttcca agcttccact tcactgcttc tgaagaaacg aaacacgaag tgtacgcgta
     1381 tccttttccg tcgaattcag tatgaagggg accgaggcat ggtttctgaa cttcaccggc
     1441 cagagtagct tcatacttca aaactggaat tccaaagaat tcgccgctga acgctttgtc
     1501 ttcgattgcc ttgagcattt tggagaaaga gtgaactgga atcacagtgg cgagttttcc
     1561 acccgcaaca atgtattcac ctgttggatc cacgtcagaa ccgtgagggc tcttaggtgt
     1621 tggcatatag aataccatgc cagggcaatc ctttggatcg agcatcttcg tgccgttcag
     1681 cgattctgat acagcttcct gattctcttc tctgtaattg tggcggtatg ctccagggaa
     1741 catacgagcc ttgccctggt ctacgcaggc ctttgccttt ttccagttga tcgccattac
     1801 aaagtctttg tctttcttgg aagcattgac ttcgagcatc ttgtaagcct gctctgtgtt
     1861 gtaagaagaa atgaagcacc agtcgtgtga aggaccttta ccgcatcttc cgaggtcata
     1921 agaaatacct ggaagcagaa cctggaagtc gattgacatc tttccggttg tcttgtcaat
     1981 gctgacgaag ctcatgcctg ctttcatttc cttctggaat tgttcccatg ccattggcac
     2041 gtttggaatc ggaattgaga atcgcgttcc agccagagca tactctgaat tctcagtgag
     2101 gaatgaagat gggtggttac ccgcgctgtt tggcagttca atgatctcta cggtctcaaa
     2161 agacttcaga tcaatgcgcg caatgcgagg agtgttattt ccgttgatga acaaccatct
     2221 tccgtcctgc tttccttctg ttacggaaag ctgagggtga tgtgaatcat cccatggaac
     2281 aaaaccgtgc gacgtcatca gcatgttctt cgtggtttcg tcgtatccat atcctgtttc
     2341 cggccattga gaaaaaaccg gaatcactcg gatcaaacga ccggacggaa ttccattcac
     2401 gcccacctgg ccgctgaatc cgccggaagc gaacatatag atttcgtctt tgtctcctgg
     2461 ctttacgtat actctttctg ctgcatcgga agcgagtccg gcagtacctg cctttccaca
     2521 cgctacgaat gcaacgagag caaagaatac gctgctaatt atttgcaatc gtttcattgt
     2581 cgtcctccta tttcgcgcca tctttcttac ggaaatgttc cagaatcata cgcgcttctt
     2641 cctgagtaac attctggaat gtcatctgcg tcagatgctc ttcgagaagc ttctgagcag
     2701 ttggatcttt ctgagtcatt tcaaccggat tgaggatcat gttcatgatc cattctggtt
     2761 ttcttctggt ggttacgccc tgaagtgcgg gaccaaccac cttctcttcc catttgtggc
     2821 aggcggagca tttcgcctcg aagagatccg ctcccttctt cgccatgcct tcatcgatac
     2881 cggcagcgat ctgtacgctg ctaacgggac cgattccggt cccagaatca ttgctcgcgt
     2941 tgtcacctga ttgcttcttg catgctgcaa gtgcaacagc caggaacagt gcagtcacca
     3001 gtgctgtgcc tattttcata cggttcatca tttcaactcc cggggacttt ctccctttga
     3061 tttcaggaac cgaaaggagc gcagagagtc aaatgaatgg gcgttgatta cgccataaga
     3121 actgtatgac atttgttcag aggctacttg acgcagatca agacgcaaaa agcgggcaaa
     3181 aaaaaaggcg ggccgcgccc gccttctcac tcgaaggtga aagattttac ttcttttctg
     3241 gtggcagaag aaccttgtcg atcacgtata ccatgccgtt agaagcaggc acagcagcca
     3301 gaacattggc tccatttatg ctgattttcc cgccttttac gctgattttt gcagttccgc
     3361 catcgaacat ggaaagctca tctccgtctg taagcatgga ctctttgaga gagcttccga
     3421 catagacgtg atatcccaaa actgtggtga gcagattctt atctgccatc actttgtcga
     3481 gtgcaccgga tggcagagct ttgaatgctg cgtctgtggg tgcgaaaact gtgaatggtc
     3541 cctgattcgc gagagagttt tccaatcccg cagcctggac tgccttcacc agagttgtgt
     3601 gatctttaga accggctgcg atttgcaaaa ctgtcttcgc cgattcctca tccgaaacgt
     3661 tcgcaatccc gccgcctgct ggacctgatt tatctttgca agcagtcgcg aataacgcta
     3721 cggaaagaag caatgtaatc cattgaagtc tcatagtgac tcctcctttg tatttatccg
     3781 gacggacctg gccatccgcg cttacggaag aacggcgcgc gcgaatcagt caaaaacttt
     3841 tccaacttcc gcgtcaggca aaaacatgat ttacatcata catcgcgagt ctcatcgtgc
     3901 ggcgtcatgt tcaaaacctc ttttgtcttc ctattgttgg cgttagtggc tcaatgttca
     3961 cacgatcgat ttgcaatttc cggatctccg gtcaaaggcc cggctggagc gagagctctt
     4021 ttcgaattca cagattttga ttgtgctccc tgtcgtgagg tgcaagagac tctgagaaga
     4081 gcgcaggcag cacaacctgt gcgactcgta ttcaaaagcc tgccgttagc cgacactgag
     4141 gcaggaaggc tcccgcatct ggtagcattc tgcgttcacg cccagaacaa agacgcattc
     4201 tggctttact atgatctcat gttttctagt tccgcacctg cgcgggacaa agactttcta
     4261 actactgttt cgcgaaccct ggacctggac gaagaagcca tgaagaagtg cgttggttcg
     4321 gaggaagcag catccgctct aaaaaaggat cgcgcagatg cacaggcggc cggagtggca
     4381 ggcactccca ctttcttttt gggaaacgct cgcctggaag gcaataagtc cacagcagaa
     4441 ttcctggcgt tcgtgaaagc caatcggtag gacgcttcag aaaccctgca atggaagagt
     4501 ggcagaagtg cgatcgcccg aagagctcgg gcgatcattc aaaaagagtt gagaattact
     4561 tgattttgct tactgcgtcc tcaaacgcct ttcttctttc gtaagcagca gcttcctctt
     4621 cgccgagaat ctgaattctg attgccttca gcttttctgc cttctcagct gccggcatgg
     4681 cggcattctg tgccagaaaa tccttctgtt tttgctcaaa atcagccagt ttcttttgct
     4741 cggccgcttc ttcttcctga gctttcgcaa tggctgcagc tgcgtctttt ccaaaatact
     4801 gttcctgaat tgcctgcgtg cgctttgcgc gatccgcatc tgacagtttt gccagatcgt
     4861 tttcgcgcag agtcagctct gtctggtaat tctggaactt atcttcgcgt ttgatctccg
     4921 catcgtaata ttgaccgaac gtttgctttt tgagagcttc atactgacga actctttcat
     4981 ccccactcaa attcttggaa gcattgatct ggcgctgaga aaggccaatg aaatcgacct
     5041 gagcttcttc cattccaaag atgagctccg cgtcttcttt gcccaggatg tcgcgccgct
     5101 tctttttcat ggtatcgtat ttttcattga agctagaatt cgcaccaaga tccgtatcgc
     5161 gcatttccgc ttcgtactgg aagtatttct taaaaagaga aagcatcttt tctttctcag
     5221 gtgagctgta gtgtttttct atgtaagaca gtatggatgc atcgcagtcc tgagcgcttg
     5281 tctcttccgc gcatttcgca cgcatggccc agacttccca aacaaagttc acatcgcctg
     5341 ttttcaactt ctgcaca
//
LOCUS       DAORFF010000238         5344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8530_length_5344_cov_3.388542, whole genome shotgun sequence.
ACCESSION   DAORFF010000238 DAORFF010000000
VERSION     DAORFF010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8530_length_5344_cov_3.388542"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PKK76_10480"
     CDS             complement(<1..849)
                     /locus_tag="PKK76_10480"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNL68943.1"
                     /translation="MRRVYKIVRIPLLLLVVGLIWSNVRYGIINRPLPVDPAWTAQAK
                     NVTIIRDEWGVPHIKGKTDADAAFGLAYAHCEDDFKFIQGGMVAARGDLARLVISKEA
                     VVNDFYVRLFKIRERADRDYDKISPDFRKVLEAYANGVNYYAATHPSEAKSKFFPASG
                     KDIAAGFIHKIPLFIGADKAMQKIMGDQPLRVDDPLDNAVAFQGLPVGSNAHALSHTR
                     TADGATYLNINSHQPWEGPVAWYEAHVQSEEGWNMTGGTFPGAPFILHGHNDHLGERG
                     EPDQGTQ"
     gene            complement(873..2306)
                     /locus_tag="PKK76_10485"
     CDS             complement(873..2306)
                     /locus_tag="PKK76_10485"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HNL68944.1"
                     /translation="MNAILCVVGSIPFFWAAFAPGSFEFVRDSGRIIQLSIVVLLVIL
                     AWIRHRDPESTLPAFLESKHWHLLLIVASSLHLLVFTESARTSLYFQDQDITNISSAI
                     ANTATGHGLLPTAFLQTGKAGSYLGHHFAPALLFFAPIYAAFPQTDHSIYPRILWIVF
                     TMGAWIWMKIVHKSLPENKQGIVLFLIFSAFPVQRVEQSFHNEILVILFSGIAFLGLV
                     TNRKFWFWFGIILWMTVKEDMAVYTGLLGAVTFVWPHPHLNRREGAILSVCSLVWLIG
                     SRLAQSHFGGGEGPAWSMFFNADWPAQSLQPVTAKWKATVLVLGAFGFLAFRRIRYAA
                     LIVPIMMIHLVSQHPWHSTYYGHYAYSFLPFLMLTATEAAKETRLTWLLVLTTAACYI
                     NAGDRETPSPPFKADPAYESLREKAQSLPADVCIHAEKHLSALVPVSVLVFPLEPFPG
                     NPHRNGFGLDQECKKHILFEPNQPVDK"
     gene            complement(2303..3688)
                     /locus_tag="PKK76_10490"
     CDS             complement(2303..3688)
                     /locus_tag="PKK76_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68945.1"
                     /translation="MLSHSNRSASGMIWAFPAGQYGGLWALARTDGYSPVSHIWLETP
                     CDVSDHYPDRFTGSPGESFYLWNTAIERSIPVLVRLHRASICLFEVIPEKPMRAGDTY
                     VLVVRDVLNRSGNPVVLSHLFDKGADYETLLSERLVPMPSNFRKFGVQTILHITIRSY
                     ENMIGQFLHLRNSILAWTDPDDVPVSITEILQNPGQLIVHGTAQLPTICERDLFGHCR
                     ITPSDTGRIEYNRSTITRDFFVRIPETTARRGLFIPAMVYQEPETLIQLNGVARLLDD
                     TGSAAFGFRDQKNFLSLNRKPVYDLPSPLPGYILDQANHDRLTAMPDREAVRVLHGIL
                     LSRLMQSRIPALISGRVKRPLRIDKIEGLALEADRDAFALSVNPFLSGDVLLFADDEP
                     RGLPFGPGGTIAEKEYVRLYWDGMHFPNYAPLIEGRGTMPDTPRRKTLVVRSILIEEE
                     RKKVVRFLNGP"
     gene            3918..4517
                     /locus_tag="PKK76_10495"
     CDS             3918..4517
                     /locus_tag="PKK76_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LipL21"
                     /protein_id="HNL68946.1"
                     /translation="MKKWYLIGAIALSSMVLYCGGSGNDARRDATSTGPDGWIFEGWA
                     CAPDAAKALLKQSPAQYCQDDKPKDFLYMKFSAAASQNAIDKGMVAMKQSTCRKAAKD
                     QISSDGLAKILGEYLEQASGVKDGQSTGQVIIAQNQGIIRGVSIYNCCSLDQQTGVCA
                     TAGAAETWDQCQCVGVMKYPGGQKGFEAAAEKASSSANP"
     gene            4663..>5344
                     /locus_tag="PKK76_10500"
     CDS             4663..>5344
                     /locus_tag="PKK76_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68947.1"
                     /translation="MARIPLLCILFLGSCARMNEATLDLPSDWRIVSGFRDAANPLTD
                     GMKVRLPVRPNEIFPDFKGSTAHYTLETTFALTPDEITRLPDPAFFFPMLGEGYNLYL
                     NGNSIVENLRLHPDGELSAVTTGRGLIVPFPSHLLRAGENRLSIHLAGFTRRTALSSN
                     SVDFGLPVKGGYRLASHTTLMRENSEMALYAFCGVYAVFAFYSLLYFVRRPKHIYTLY
                     FALFTCTYS"
BASE COUNT         1289 a         1322 c         1445 g         1288 t
ORIGIN      
        1 ctgggtgccc tggtctggct cgcctcgctc accgaggtga tcgttgtgcc cgtgcagaat
       61 gaaaggcgct ccaggaaaag tgccccccgt catattccac ccttcctctg attgtacgtg
      121 agcttcgtac caggcgactg gaccttccca cggttgatgt gagttgatat tgagataggt
      181 tgcgccgtct gcggttcgcg tgtgcgaaag agcgtgagcg ttcgaaccaa cgggaagccc
      241 ttgaaatgcc acagcattgt cgagaggatc gtccacgcgc agtggttgat cgcccataat
      301 cttttgcatt gctttgtctg cgccaatgaa cagcgggatt ttgtgaatga acccggcggc
      361 aatgtctttg cccgatgcgg gaaagaattt gctttttgcc tctgatggat gggtagcggc
      421 gtagtagttg actccgttcg cgtatgcttc cagtactttg cgaaagtccg gcgaaatctt
      481 atcgtagtct ctgtccgcgc gttcgcgaat cttgaagagc cgcacgtaga aatcgttcac
      541 taccgcttct tttgaaatca cgagtctggc cagatcgccg cgcgcggcga ccattccacc
      601 ctggatgaat ttgaaatcgt cttcgcagtg cgcgtaagcg aggccaaatg cagcatccgc
      661 atccgtcttc ccctttatgt gaggaacacc ccattcgtcg cgaatgatgg ttacgttctt
      721 tgcctgcgcg gtccatgcag gatcgactgg caaaggccga tttatgatgc cgtatctgac
      781 gttagaccag atgagaccca cgaccagaag gaggagcggg attcgcacga ttttgtaaac
      841 gcgtcgcata acagggattt agccctgatt gctcatttgt caacaggttg gttgggttca
      901 aacaggatgt gtttcttgca ttcttgatcc aggccgaatc catttctgtg cggattgccc
      961 ggaaacggct cgagaggaaa gaccagaaca ctcacaggaa cgagtgcgct gagatgcttt
     1021 tctgcgtgaa tgcatacatc tgccggcagg ctttgggctt tttcgcgcaa agattcatag
     1081 gctgggtctg ctttgaaagg aggtgaggga gtctcacgat ctccggcgtt gatatagcag
     1141 gcagctgtcg taagcaccag gagccatgtg agcctggttt ctttcgccgc ctccgtcgcg
     1201 gtaagcatga ggaagggcag aaaagaataa gcgtagtgac cgtagtacgt agaatgccat
     1261 gggtgttgcg aaaccaggtg tatcatcatg atgggcacga tcaatgccgc ataacggatt
     1321 ctgcgaaatg cgagaaaacc aaaagcgccc agcacgagca cagtggcttt ccatttcgca
     1381 gtgacgggct ggagggactg tgccggccaa tctgcgttaa aaaacatgct ccacgccggt
     1441 ccttcgccgc caccgaaatg cgactgagcc aggcgacttc caatcagcca caccaggctg
     1501 caaacagaga ggatcgcacc ttcgcggcgg ttcaagtgag gatgtggcca gacaaaagtg
     1561 actgccccca gtagaccggt gtaaaccgcc atgtcctcct tgacagtcat ccagagaatg
     1621 attccgaacc agaaccagaa ttttcgattt gtgaccagtc ccaggaatgc gattcctgag
     1681 aaaaggatga ccagtatttc attgtgaaat gattgctcta ctctctgcac aggaaacgcg
     1741 gagaagatca agaacaggac gattccctgt ttgttctctg ggagagattt gtgtacgatc
     1801 ttcatccaga tccaggcacc cattgtaaat acgatccaga gaattcttgg gtagatgctg
     1861 tgatctgttt gcggaaacgc tgcatagatc ggagcaaaga agagaagcgc cggagcaaaa
     1921 tgatgaccca gataactccc cgctttccct gtttgtagaa acgcagtggg caggagcccg
     1981 tgaccggtgg cagtgtttgc aattgcagaa gatatgttgg taatgtcctg atcctggaaa
     2041 tagagagacg tgcgcgcgct ttccgtgaac acaagaagat gaagggatga agctacaatg
     2101 aggagcagat gccagtgttt gctttccagg aatgcgggca gagtggattc aggatcgcga
     2161 tgacgaatcc aggcaagaat tacgagcaaa actacaatag aaagctggat gattctcccc
     2221 gaatcgcgca caaattcaaa cgatcctggt gcgaaggcgg cccagaagaa tggaattgag
     2281 ccgacgacgc acagaattgc gttcacggtc catttaagaa tcgcactact ttcttgcgtt
     2341 cttcctcaat caagatgctt cgcacaacca gagttttgcg gcgaggagta tccggcatgg
     2401 ttcccctgcc ttctatgagc ggggcgtagt ttgggaaatg catgccatcc cagtagagtc
     2461 gcacatattc tttttctgca atcgttccgc ccggtccgaa tggcaatccg cgcggttcat
     2521 cgtctgcgaa gagaagaaca tctcctgaga gaaatggatt cacggataga gcgaatgcat
     2581 cgcgatcagc ttccagagcc agaccttcga tcttatctat gcgaagaggt ctcttcacac
     2641 gcccagatat caacgcggga atccgtgatt gcattaaacg agaaagtaga atgccatgca
     2701 gtacgcgaac agcttcacga tccggcatgg ccgttaagcg atcgtgattt gcctgatcga
     2761 gaatataccc tgggagagga gatggaagat cgtagactgg ttttctgttg agtgacagaa
     2821 aattcttctg gtctctaaac ccgaacgcag cagatccagt gtcgtctaac agccgcgcca
     2881 cgccgttgag ttgtataagt gtttccggtt cctggtaaac catcgcagga atgaatagac
     2941 cccgacgagc cgttgtttcc ggaattcgca caaagaaatc gcgtgtaatt gtagatcgat
     3001 tgtattcaat tctgcctgta tcagatggcg ttatgcggca atgcccgaac aaatcgcgct
     3061 cgcatatcgt gggaagttgt gcggtgccgt ggacaatgag ctggcccgga ttctgaagga
     3121 tttctgtaat gcttaccggt acgtcgtccg ggtctgtcca ggcaaggatg gaatttcgca
     3181 gatgtagaaa ttgtcctatc atgttttcgt aggatcgaat cgtgatgtgc aggatcgtct
     3241 gtactccgaa ctttctgaaa ttggaaggca tcgggacgag cctttcgctt agaagagttt
     3301 cgtagtccgc tcccttatcg aatagatggg agagcacaac cggattcccg gatcggttca
     3361 gtacgtcgcg caccacaagt acgtatgtgt ccccggcgcg catgggcttt tccgggatca
     3421 cttcaaacaa acagattgat gccctgtgca atcgcaccag aacgggaatg cttctttcga
     3481 tggccgtgtt ccagaggtaa aaggattcgc cgggtgaacc agtaaatcga tccggatagt
     3541 ggtcggagac gtcacagggg gtctcaagcc agatgtgcga aactgggctg tagccgtccg
     3601 ttcgggccag agcccacaat cccccatact ggcccgccgg gaaagcccag atcatgccgg
     3661 atgcacttct gttggaatgc gaaagcattc gatcgtccgg gaaggggaag ttgcaatccg
     3721 agagagacag acggcagtcc cccggcctgg catgggtggg gaatgaagcc aggatgagaa
     3781 taactctcag agctataaaa acgattcggg tattttgaat ttttcttgaa cgcagggacg
     3841 gaaagcctcg tctgatcttg tatcaggaag ccggagtggc ttcccatcaa gtttctatta
     3901 ggggacagga gaagtctatg aagaaatggt atttgatcgg ggcgattgcc ctgtccagca
     3961 tggttctgta ttgtggcggt agcggaaacg atgcacgtcg tgacgcgacg agcaccgggc
     4021 cagacggttg gatttttgaa ggttgggctt gcgcaccaga cgcagcaaaa gctctcctca
     4081 agcaaagtcc tgctcagtac tgccaggatg acaagccaaa ggactttctg tatatgaagt
     4141 tcagcgcggc tgcatctcag aatgcaatcg acaaaggcat ggttgctatg aagcagtcta
     4201 cttgccgcaa agcagccaag gatcagattt catctgacgg tcttgcgaag atcctcggtg
     4261 aatacctgga acaagcatcc ggtgtgaaag acggccagtc cacaggtcag gtcatcatcg
     4321 cgcagaacca gggtatcatt cgtggcgtgt ccatctacaa ttgctgttct ctcgaccagc
     4381 agacaggtgt ttgcgcgact gccggagctg cagaaacctg ggatcagtgc cagtgtgtgg
     4441 gtgttatgaa atatccaggc ggccagaaag gtttcgaagc tgctgctgaa aaagcatcca
     4501 gctctgcaaa cccgtaattc aaaactcgaa ttgcgacaaa agaagccccg gtctaaaagc
     4561 cggggttttt ttgtgtccag ggtcaacgaa cgttaggcga aaacacacgc aactggaatg
     4621 ttcttgcagt aacttgtatt gtgcaaagtt cttgtgacac gcgtggctag aattcctctc
     4681 ctttgcattc tgttccttgg ttcttgtgcg cggatgaacg aggccactct agaccttcct
     4741 tctgactggc gtattgtctc cggatttcga gacgcggcga atcctctcac tgacggaatg
     4801 aaggtgcgac ttcctgttcg cccaaatgaa atcttccctg attttaaagg ttcgacagcg
     4861 cactacacgc tcgaaactac atttgcgctt acgcccgacg aaatcacccg cctccctgac
     4921 ccagctttct tttttcccat gctgggggaa ggttacaatt tgtatttgaa cgggaattcc
     4981 attgttgaga accttcggct tcatccagac ggagagctgt ctgcagtcac tacaggtcgc
     5041 ggactgatcg ttccatttcc gtcgcacctg cttcgcgccg gagaaaatcg gctgtccatt
     5101 caccttgcag gattcacgcg aaggactgcc ctttcatcca attcggtaga ctttggttta
     5161 cccgtgaagg gcggctatcg cctcgcttcg cacacaactt tgatgcgcga aaattcggaa
     5221 atggcacttt acgcgttttg cggcgtctac gcagtttttg ccttctacag tctcctctat
     5281 tttgttcgcc gtccaaaaca tatttacact ctctatttcg cgctcttcac gtgtacttat
     5341 tctg
//
LOCUS       DAORFF010000239         5339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8550_length_5339_cov_3.769871, whole genome shotgun sequence.
ACCESSION   DAORFF010000239 DAORFF010000000
VERSION     DAORFF010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8550_length_5339_cov_3.769871"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PKK76_10505"
     CDS             <1..581
                     /locus_tag="PKK76_10505"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL68948.1"
                     /translation="LRIHPSGRFMYTTNSGAANISAYSLSVGAPTNILTTASGGTSPQ
                     GIALDPTGNFLYVANSGGTPNIAIFSINQSTGIPTLQTTINSTSVYGIVVDPTGQYAY
                     TTNQGASNTVSTYRRDSAGLLTLVGTVAAGTAPAFLAIDPQSKFLYVSNNNTATQYVS
                     QYRIVSGLPVSIGTIAAGDGGYFVDLLVKYFY"
     gene            complement(596..2161)
                     /locus_tag="PKK76_10510"
     CDS             complement(596..2161)
                     /locus_tag="PKK76_10510"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNL68949.1"
                     /translation="MASEDAASGEVSAIPYLAGFKDNARNWTARDVVRADFKPVAGAI
                     LDEGFSDAAHWLKFSTPRTSGKQSMFLVFESLTLDRAELYSLENPESAVARAGDFYPR
                     SSWAVPWSDYPTFPVESGKSYLIRLQSTSLLMMPVTLRTESNLRGFTERRTFLSALYA
                     GFTLMLCLVAFGIFLALRDFVYLLYVGYVASQTLAFAALFSSFYRLLWPESSYIQNHL
                     YFFAQALSLAFGTSFFRAFTDLAERRPRLNILVTSVTVLSLIIAIGTLFSAQNVIFSR
                     SLTVIYLLWIPIFLFLTMRQIEPGRIDMWMFAFVWGAVYLAGILYMLSAGRIIPFHSL
                     FFYGQTAFFPFDALFFILSLYRRYRTMESARLDLEREMHSTLAKLASQSRERSAKKKV
                     GYSRSKLSGVNVGKILESLDHSLRTEKLFKKEDLSLAELASHIGITPHQLSEICNAQL
                     NTSFPKLLAYHRVLEAKELMANSDLNILQIAFDSGFASKSSFNIEFKRVTGLTPREYR
                     ARPDVRHDFASAV"
     gene            complement(2333..4066)
                     /locus_tag="PKK76_10515"
     CDS             complement(2333..4066)
                     /locus_tag="PKK76_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68950.1"
                     /translation="MHLIQRFAFPFLVLFFSLIGSSHAQSESNFQPLEQHKFAEYELY
                     HYHAQLFGGWGPGNYPLIPMGVRIYFIPRTFDYPCCQSGVFLSAPRQAPHPSNYSIRM
                     DGFTGSPQGAEISLEAYEIGFSNDSESASDRFPNEKGMLYGGTWLTFTKIARVNPLLR
                     TPESDPNTHYLLRFQVQQPDFSIRLKQMPFQDFPNHPWPDASEFRDYGYTDEIGKSGM
                     VMLSGRLLLSPGLPRLASASPWTGPEVVNANTGIRTLRPAYALVAKNGWQSHYDLVAR
                     LSRGGSARDEYRSVKYMNFEPDANHTVIRFTGMIRYGTQAGDKPELLGEWMRKTTFSL
                     RKKPWGSLMASMNAYACVRPECEIKPMEVPSVQVFDTDAKENSDSLRINPDFRLGASR
                     YLNPNVGPYGDLNVPARLSFRLRSADGGALEGVSLEISASKADASIQIPKPAVTVGAM
                     FHKFLTLKYDPNFDPPMVGQIVPGFPARMEARQMVELDDAPGVRYKINCTVVIGSQRH
                     SCSRGSPGSVVTAVRQILPPEAGKTEGRARSFQRFEDFSSYTLGEHYTGADYDPVLDA
                     YPLFEIEVRKK"
     gene            complement(4221..>5339)
                     /locus_tag="PKK76_10520"
     CDS             complement(4221..>5339)
                     /locus_tag="PKK76_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL68951.1"
                     /translation="LVAAALPDFVSAEDRDRIFHFHVPMKGARGGHGLTLRSKRPIYF
                     KDFGEKDLRWLTDEERYVLDTCRIKSYLFLPLLLNGEPVGMLNFSNSDGPLNLKDEDL
                     TRFSILGEQMAGIITGSALFKEVQAARNSAESERKKSEKLLLNILPADVASELKEKGA
                     TDPVLYENVSVMFTDFKGFTQIAETLSPRELIQDLDACFVQFDKVTERFNLEKLKTIG
                     DSYMCAGGIPRRNKTHAIDCVLAALEIQAFMNLMKQIKTEQGLPYWELRLGIHSGPLV
                     AGVIGEKKFAYDVWGDTVNTASRMESSGTPGRINISGSTFELVKDVFDCEFRGYVSAK
                     NKGEVAMYYVNGLKEEYSLAQDAKAPNGNFWTVYATVA"
BASE COUNT         1448 a         1393 c         1255 g         1243 t
ORIGIN      
        1 ctctgcgcat ccatccatcc ggccgattta tgtatacgac gaactcagga gccgccaata
       61 tttccgctta ctcgcttagc gtcggagctc caacaaacat ccttacaacc gcgtccggag
      121 ggacttcacc gcaggggatc gcgctggatc ctaccggcaa ttttctatat gtcgcaaatt
      181 cgggcggcac acccaacatc gccatcttct ctatcaatca gagcacaggc attccgaccc
      241 tgcagactac tatcaactca acgagcgtct acggtatcgt agtcgatcca acgggtcagt
      301 acgcgtacac aaccaatcag ggtgcttcca atactgtgtc cacatacaga cgcgacagtg
      361 cgggacttct cacgttagtc ggcactgtag cggccggaac ggcaccggca tttcttgcga
      421 tcgatccaca gagcaaattt ttgtatgtga gcaacaacaa tactgcaacg cagtacgttt
      481 cacaatatcg aatcgtttca ggccttcctg tttccatagg aaccatcgca gcgggagacg
      541 gcgggtattt tgtagatttg ctggtgaaat atttctactg agtaaaggat gattgtcaca
      601 cggctgaagc aaagtcatgc cgcacatccg gccttgccct gtattcgcgc ggagtcagcc
      661 ccgtgactcg cttgaactct atattgaaag atgatttaga tgcgaaacca gaatcaaaag
      721 caatctgcag aatattcaga tcactgtttg ccatcagctc ttttgcttcc agaactctat
      781 gatacgccag caacttagga aaactggtat tcaactgagc attgcaaatc tcagacaact
      841 gatggggtgt gatgccaatg tggctggcaa gttctgcaag agagagatcc tccttcttaa
      901 atagcttctc cgttcgcaaa gagtgatcca gactttctag aatttttcct acattcacac
      961 cggatagttt gctcctggaa taaccgactt tcttcttcgc ggatcgctcg cgagactgag
     1021 aagcaagctt cgcaagagta gaatgcatct cgcgctccag atccagacgt gcactctcca
     1081 tcgttctgta tctcctgtac aaactgagta taaagaagag agcatcaaac ggaaaaaatg
     1141 cagtttgacc gtagaaaaaa agcgaatgaa acgggatgat gcgtcctgcg gaaagcatgt
     1201 atagaattcc tgccagatac actgcccccc agacgaatgc aaacatccac atatcaatcc
     1261 gtcctggttc gatctgtcgc atggtcaaaa agagaaagat tggaatccag agcaaataga
     1321 tcacagtgag tgaacgggag aatatcacat tctgggctga aaacaatgtg ccgattgcta
     1381 tgatgagaga aagaacagtc actgacgtaa caagaatatt gagcctcggc ctgcgttccg
     1441 caagatcagt gaaggcgcga aagaaagaag taccaaacgc aagactcaga gcctgcgcga
     1501 aaaaatacag gtgattctgg atgtaggatg attccggcca gaggagcctg taaaaagagg
     1561 aaaagagtgc cgcaaacgca agagtctgcg atgcaacgta tccaacatac agcaagtata
     1621 cgaagtcgcg caatgcgaga aaaattccaa aggcaacaag acagagcatc aatgtaaatc
     1681 cggcataaag cgcgcttaag aaagtgcgcc tctctgtaaa gccacgcaag ttcgattcag
     1741 tgcgcagagt cacaggcatc atcagaagag atgtgctctg caaacgaatc aaatagctct
     1801 taccagattc aactggaaat gttggatagt cgctccacgg cacagcccag gaagatcgtg
     1861 gatagaaatc acctgccctt gcaacagcag actccggatt ttccagggaa tagagttccg
     1921 cccgatccag agtgagcgat tcaaacacaa gaaacatgct ctgcttgcca cttgtacgag
     1981 gtgtggaaaa ttttagccag tgagctgcgt cagaaaatcc ttcatctaga attgcgcctg
     2041 ccacaggttt gaaatctgca cgcactacgt ctctggccgt ccaatttcga gcgttgtctt
     2101 tgaagccggc caggtacgga atggcagaga cttcaccgct tgcggcatct tctgaagcca
     2161 cagaacaacc cgaagacaac aaacacagcg taaagcaaca accaatggcg ttggttcgaa
     2221 tccggggcat gcggtgcagt cggcatgacg agtccaggcc gtaaacactg tttttgaaac
     2281 gatcgcctaa cgctatgatc agcgggcctt ccgcgaggcc ccgcgagctg ttctattttt
     2341 tgcgaacttc aatctcgaag agcggatacg catcgagcac aggatcgtag tccgctcctg
     2401 tgtagtgctc tcccagagtg taagagctaa agtcctcgaa tcgctggaaa ctgcgcgcgc
     2461 gtccctccgt ctttcctgcc tcgggaggca gaatctggcg cactgcagtc acaacggatc
     2521 ctggacttcc acgactgcag gaatgtctct gcgacccaat gaccactgtg cagtttatct
     2581 tatagcgaac gccaggtgca tcatctaact ccaccatctg tcgggcttcc attcgtgcgg
     2641 ggaatcctgg cacaatctga cccaccatcg gcggatcaaa atttggatcg tatttcaggg
     2701 taagaaattt atgaaacatc gcacccactg tcactgcagg ctttgggatt tgaatggaag
     2761 catcggcttt cgaggcactg atttctagag acacgccttc gagggcccca ccatccgctg
     2821 agcgcaagcg aaagctcaac cgggccggta catttagatc tccgtacggt ccaacgttcg
     2881 gattcagata tctacttgct cccagacgaa agtctggatt gatcctgaga ctgtccgaat
     2941 tctctttcgc atctgtgtca aagacctgaa cagacggcac ttccattggc ttaatttcgc
     3001 attctggacg aacgcacgcg tatgcattca tagacgccat cagagaaccc cacggttttt
     3061 tccgtagaga gaatgtagtc tttcgcatcc actctccgag caactctggt ttatcgcccg
     3121 cctgagtgcc gtagcggatc atacctgtga agcgaataac ggtatggttc gcgtccggtt
     3181 cgaaattcat gtatttcacg gagcgatact catcgcgagc gctaccgccg cgcgaaagac
     3241 gggccaccag atcatagtga ctctgccatc cattcttcgc gacgagcgcg tatgcgggac
     3301 gtagtgttcg aatgcctgta ttcgcattca ctacttctgg tccggtccat ggcgatgcgg
     3361 aagcgagtcg aggtaagcca ggggacagaa gaagccgacc agagagcatc accatcccgg
     3421 attttccgat ctcgtctgtg taaccatagt cacgaaattc ggaagcatct ggccatggat
     3481 gattcgggaa atcctgaaaa ggcatttgtt tcaagcgaat gctgaaatca ggttgttgca
     3541 cctggaaacg aaggaggtag tgtgtatttg gatccgattc aggcgtgcgc aacagtggat
     3601 tcactcgcgc gatctttgtg aacgtaagcc atgtgccgcc atatagcatg cctttctcgt
     3661 ttggaaagcg atcactcgcg gactcagaat cgttcgagaa accgatctca taagcttcca
     3721 gagaaatttc tgcaccctga ggcgaaccgg taaaaccgtc catgcgaatc gaatagttgg
     3781 atggatgcgg agcctggcgc ggcgcgctca aaaagactcc gctctgacaa caggggtagt
     3841 caaaagttcg cggtataaag tagattcgca cgcccatggg aataagcgga taatttccag
     3901 ggccccatcc tccaaataac tgagcatgat aatgatagag ttcatactct gcaaatttat
     3961 gttgttccag tggctggaaa ttgctttcgc tctgagcgtg cgaagaacca atcaggctaa
     4021 aaaacaaaac aagaaacgga aaagcgaaac gctggattag atgcatgcgc aaaagcacat
     4081 gccaggagga cacatctcaa gcagaaaacg gattctaact cacgagagga tgagatggat
     4141 tcgcatttta aaacagatca gataaatgtt cgggggaaat tctcccctat cgttttactt
     4201 aaacaagtag cctaacctca tcaggcgacc gtcgcgtaca ccgtccagaa attaccgtta
     4261 ggcgcttttg catcctgagc cagggagtat tcctctttga gcccatttac gtagtacatg
     4321 gccacttccc ctttgttctt cgcactgacg tagccgcgaa actcacaatc aaaaacatcc
     4381 ttcacaagct caaaggtcga accgctgata ttgatcctgc ccggagtccc cgaggattcc
     4441 atgcgagatg cggtattgac tgtatctccc cacacatcat atgcgaattt cttctctccg
     4501 atcacgcccg caaccagcgg gccagagtga atccccagcc tcaactccca gtatggaagg
     4561 ccctgctctg tcttgatctg cttcatcaga ttcatgaatg cctgaatctc aagcgctgcc
     4621 agaacacaat cgatcgcgtg cgttttattt ctcctgggaa ttccaccggc acacatatag
     4681 ctgtctccaa tggttttcag tttctcaaga ttgaatcgct ctgtgacttt atcgaactga
     4741 acaaagcaag catccagatc ctgaatgagc tctcgcggag agagtgtctc cgcaatttga
     4801 gtaaaccctt tgaagtctgt aaacatcaca ctcacatttt catacagtac aggatccgtg
     4861 gcaccctttt ccttcagctc cgacgccaca tccgccggta gaatattcaa aagaagtttt
     4921 tccgatttct ttctctcaga ttcagcactg tttctggccg cctgcacttc tttgaaaaga
     4981 gcagaccctg tgatgatccc cgccatctgt tcgcccagga tggaaaagcg cgtaagatct
     5041 tcatctttta gattcagagg gccatcggaa ttcgagaaat taagcatccc cacaggctct
     5101 ccattcaaaa gcaaaggaag aaacagatac gacttgattc tacaagtatc gagcacgtac
     5161 ctctcttcgt ctgtaagcca ccggagatct ttctccccaa aatccttgaa ataaatgggg
     5221 cgtttgctgc gaagagtcaa cccgtgtcct ccgcgggctc ctttcatggg cacatggaaa
     5281 tggaaaatcc gatctctgtc ttctgcgctt acaaagtccg gcaatgcagc ggccacgag
//
LOCUS       DAORFF010000240         5337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8558_length_5337_cov_2.907989, whole genome shotgun sequence.
ACCESSION   DAORFF010000240 DAORFF010000000
VERSION     DAORFF010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8558_length_5337_cov_2.907989"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PKK76_10525"
     CDS             complement(<1..239)
                     /locus_tag="PKK76_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68952.1"
                     /translation="MSDPRSLSLVLFHALSGKLRRYDELTPSQARALLLESLELLPDF
                     DVHKTTVPHVVRVTTREGKSFDFNIQDFADSLVML"
     gene            complement(244..840)
                     /locus_tag="PKK76_10530"
     CDS             complement(244..840)
                     /locus_tag="PKK76_10530"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNL68953.1"
                     /translation="MRLPAVLLIALLAVSSLVSCKKSSDDSLDFATLLVLATFRDNGN
                     GTVSAPGGYTIAKCAYGQVWNAALNNCAGTGSSSTYGAQSLAYCTVANSCHDAALNAS
                     SGPAFAACSTYSVSSITGWRLPTVAELQAMTSSLSRSSLILLFTQTPDDKYFWSRETN
                     PNKSDYSEAYGISFADSTFGDKTSYNKVSVPLYVRCVK"
     gene            complement(888..1778)
                     /gene="lipA"
                     /locus_tag="PKK76_10535"
     CDS             complement(888..1778)
                     /gene="lipA"
                     /locus_tag="PKK76_10535"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNL68954.1"
                     /translation="MRYPETIPPAPPEWMKVRLKMGTPDSTSARAVREVLTRQRLVTV
                     CEEASCPNLGHCWSQGTATFMIMGDKCTRSCGFCDIRTAKPMPLDAAEPQRIAQAIQE
                     MKLRHAVITSVDRDDLADCGSTHFAAVIAATRALNPETTLEVLIPDFKGKRENLERIW
                     NEKPDIINHNVETVPTLYKSICPQSNYQVSLDVLRMSAQQGFLAKSGIILGLGEEISE
                     VKQVISDLKAAGTQLLTIGQYLQPSSAHAPLKEFVAPEVFDSLKEHALAAGFIHVEAG
                     PLVRSSYHAGDALDRIFGRK"
     gene            1864..3231
                     /locus_tag="PKK76_10540"
     CDS             1864..3231
                     /locus_tag="PKK76_10540"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL68955.1"
                     /translation="MTSQKQPFSLLRAIGLGDLAGQGFRVQFSFWSMVAMTFFLFGDQ
                     NLASPNLSRIGAAFGITDEAEYRAKIAGHASLYFFVIGGIASILVGAFTDIWNRKLLL
                     FGTVFAGELACFFTAFAPNYTTYIVLRTLTGIGLGGIFPIIFSLLGDHFKPENRSSAS
                     GWLALAMGLGIGVGQILGGMLATATVFGLEGWRASFAIMAGPCFPLLFLYLAIGETPK
                     RGGAENVHETTGEEPKHSLGLSDFKRIASSKTNILAILQGIPGCVPWGLIFFFLVDYY
                     EKSKGFKPSDGVLLTTVFGGVLIFGGFIGGFVGRAVYNWKKAALPVFCAACVLLGIVP
                     MIYILNFQGNSMLYPMIAAALGGIVIPQAGPNIRSILVNTNLPENRGSIFGVFNFADD
                     LGKGLGPFVVGEILRLVGSDSLAYNLAIYCWIPCAIGWLLMAFTMEADEKKVEDTLIA
                     RGAKR"
     gene            3247..3732
                     /gene="coaD"
                     /locus_tag="PKK76_10545"
     CDS             3247..3732
                     /gene="coaD"
                     /locus_tag="PKK76_10545"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNL68956.1"
                     /translation="MKTAVYPGSFDPFTNGHLDLVQRASQLFDRVIVSVAINSSKNSF
                     FTVEERMAILREVLASMPGVEVASFEGLLVDYCRTVGARAIVRGLRAVTDFDYEYAIF
                     QLNHDLNAEVETVFLLAAKEYSFVSSTLIKEVARYGRSVEQHAPPAVSRALLRKFGHA
                     K"
     gene            complement(3832..4266)
                     /gene="rplO"
                     /locus_tag="PKK76_10550"
     CDS             complement(3832..4266)
                     /gene="rplO"
                     /locus_tag="PKK76_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNL68957.1"
                     /translation="MKHIGNLSPASGSIGKKKRIGRGAGSGHGGTSTRGHKGHMATSG
                     YESKRGFEGGQMPLSRRLPKFGFSNPFRVEYQVLNLGTIQELAAADKFQGSSVTPELL
                     YEIGAISKRNVENPALFVPASSKAANMAAKNAVIPSRRKMMA"
     gene            complement(4281..4466)
                     /gene="rpmD"
                     /locus_tag="PKK76_10555"
     CDS             complement(4281..4466)
                     /gene="rpmD"
                     /locus_tag="PKK76_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNL68958.1"
                     /translation="MEKIKITQTRSIIKCLEKQKRTIKALGLGRPNYSVIQPKNTQIL
                     GMIAVVNHLVSVEDITA"
     gene            complement(4488..4991)
                     /gene="rpsE"
                     /locus_tag="PKK76_10560"
     CDS             complement(4488..4991)
                     /gene="rpsE"
                     /locus_tag="PKK76_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003194373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNL68959.1"
                     /translation="MAKIRVSEIDLKDKLVYVGRTAKVVKGGRRFNFSAIVVVGDGNG
                     HIGYGLGKAGEVVDAVTKATDAAKKNVVSVRLQDTTIPHDVVGKFGAARVFIKPATPG
                     TGVIAAGGVRAVLELAGVQDVLTKSMGSSNPHNTVKATIKALTQLEDAQSVSARRGIS
                     VEKVMRG"
     gene            complement(5009..5335)
                     /gene="rplR"
                     /locus_tag="PKK76_10565"
     CDS             complement(5009..5335)
                     /gene="rplR"
                     /locus_tag="PKK76_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNL68960.1"
                     /translation="MRVRKKISGSPERYRMTVYRSLKHIYAQIIDDTQNSTLVSACTV
                     EKEAIAAMSNDTTKSDSSKIVGALIAKRALEKNITSVAFDRNGYIYHGRVKALADAAR
                     EAGLQF"
BASE COUNT         1294 a         1330 c         1287 g         1426 t
ORIGIN      
        1 tgaagcataa ccaggctgtc tgcaaaatcc tggatattga aatcgaacga tttcccttct
       61 cgcgttgtga ctcgaaccac atggggaacg gtggtcttat gcacgtcaaa gtccggcagc
      121 aactcaagag attccagaag aagagcgcgc gcctgcgaag gtgtcaattc gtcgtagcga
      181 cgaagctttc ctgaaagcgc gtgaaaaaga accagggaaa gggagcgagg atcgctcacg
      241 gcatcacttc acgcagcgaa cgtagagggg tacggatacc ttattgtagg aagtcttatc
      301 tccaaatgtg ctgtctgcga agctgattcc gtatgcctct gaatagtcgc tcttgttggg
      361 gtttgtctca cgagaccaga aatacttatc atccggagtc tgtgtaaata gcaggatgag
      421 gctggacctg ctcagagaac tggtcattgc ctggagctcc gctacagttg gcagcctcca
      481 gcctgtaata gaagaaacag aataggtaga gcaggctgca aatgcaggac cagaactcgc
      541 attgagtgcc gcatcatggc aggaattggc tacggtgcaa tacgcaaggc tctgagcacc
      601 gtaggtgctg gaagatccgg ttccagcgca gttgttcagt gccgcattcc agacctggcc
      661 gtaagcgcat ttggcgatgg tgtaaccacc cggagcggat acagttccat tcccgttgtc
      721 tctgaaagtg gccagaacca gcaaagtggc aaaatcaagg ctgtcatcag aggacttctt
      781 gcaggaaaca agagaggata cagcgagaag tgcaatcaat agaacggctg gcaaacgcat
      841 gaatttcctc atcatatata ttagacgatt agagcgagcg attttgctca ttttcttcca
      901 aaaatccggt ccagagcatc gcccgcatga tacgaggatc ggacaagagg acccgcttcg
      961 acatgaataa atccggcggc cagagcatgc tctttgagag aatcaaacac ttcaggcgcc
     1021 acaaattctt tcagaggagc atgagcactc gacggttgca gatattgccc gatggtcaga
     1081 agctgagtgc cggccgcttt gaggtcggaa atgacctgct tgacttctga gatttcctcc
     1141 cccaggccca ggatgatccc gcttttggcc agaaagcctt gctgtgcgct cattctcaaa
     1201 acatccaggg aaacctggta attcgactgc gggcatatgg atttgtacag tgtaggaact
     1261 gtttccacgt tatgattgat gatgtccggc ttttcgttcc agattcgttc gaggttttcg
     1321 cgctttcctt tgaaatcagg aatcaatact tccagggtgg tttctggatt cagtgcccgg
     1381 gtagcggcaa tgactgctgc aaaatgagtg gatccgcaat cagccagatc gtcccggtct
     1441 acggaagtga tcactgcatg tctcagcttc atttcctgga tggcctgcgc aatccgctgt
     1501 ggctcagccg catccagggg cattggtttt gcagtgcgaa tgtcacagaa accacagcta
     1561 cgggtgcatt tatcgcccat gatcatgaat gtggctgttc cctgagacca gcaatgtccg
     1621 aggttcggac aggacgcctc ttcgcacact gtcaccagcc tctggcgagt gagcacttct
     1681 cggactgccc tggcactggt tgaatccggg gtacccatct tcagccgcac cttcatccac
     1741 tccggcggag cgggcgggat cgtttcagga taacgcatca ggttcagaga aacgattctg
     1801 cggccccggt cgacgaaaaa ttgccttgtc agctttcttc tgcccccgtt tgatcccccc
     1861 gacatgacat ctcaaaaaca gccattcagc ctgctacgag ccattggcct gggcgatctt
     1921 gccggccagg gtttccgcgt acaattttca ttctggtcca tggtagccat gacattcttc
     1981 ctgtttggcg accagaatct cgcatccccg aacctctcga ggatcggagc cgcattcgga
     2041 attacagatg aggcggaata tcgtgcgaag atcgcagggc atgcgtcgct ctatttcttt
     2101 gtcatcggcg ggattgcttc tattctcgtt ggagccttca cagacatctg gaatcgcaag
     2161 cttctattgt tcggcactgt gttcgcaggt gagcttgcgt gcttcttcac ggccttcgcg
     2221 cccaactaca cgacgtacat cgttcttaga acgttaaccg gcattggtct gggtggaatc
     2281 ttccccatta ttttctcttt gctcggggat catttcaaac ctgaaaacag atcgagcgcc
     2341 tctggctggc tggctcttgc gatgggtctt ggaattggag tcggacaaat tctaggagga
     2401 atgctcgcga cagcgactgt atttggttta gaaggatggc gcgcaagttt cgcgattatg
     2461 gccggaccat gcttccctct tttattcctg tatcttgcca tcggcgaaac tccaaaacgc
     2521 ggcggagcag aaaacgtaca tgaaacaaca ggagaagaac caaaacattc tcttggactc
     2581 tccgatttta aacgaattgc aagcagcaag acaaacattc tggccattct tcagggaatt
     2641 ccagggtgtg ttccatgggg cctcatcttc ttttttctcg tagactacta tgaaaaatca
     2701 aaagggttca aaccatctga tggcgttctt ctgacaactg tgtttggagg cgttctgatc
     2761 tttggtggat tcattggagg gtttgttggt cgcgcagttt acaattggaa gaaagcagcg
     2821 ctgccagtat tctgtgctgc ttgcgttcta cttggaattg ttcccatgat ctacattctc
     2881 aacttccagg gaaattctat gctctaccct atgatcgcgg cggcgcttgg cggaattgtg
     2941 attcctcagg caggcccgaa cattcgcagt atcctggtaa atacaaacct tccagaaaat
     3001 cgcggatcga tctttggagt gttcaacttc gcagatgatc tgggcaaagg acttggacct
     3061 tttgtggtcg gtgaaattct aagactggtc ggaagcgact ctcttgcata caatctcgcc
     3121 atttattgct ggattccgtg cgcgatcgga tggcttctga tggctttcac tatggaagca
     3181 gatgaaaaga aagtagaaga cactctgatt gcaaggggcg cgaaaagata atcgggaaac
     3241 atagcggtga aaacagcagt ctaccccgga tcgtttgatc cttttacaaa cggacacctg
     3301 gacctggttc agagagcctc gcagctcttt gaccgggtga tcgtaagcgt tgccatcaat
     3361 tcatccaaga attcattttt cacagtggaa gagaggatgg cgattctacg cgaagttctc
     3421 gcatccatgc caggcgtgga agtggccagc ttcgaaggac ttcttgtcga ttattgtaga
     3481 acggttggcg cacgagccat tgtgcgtggc cttcgagctg ttacagactt tgactacgaa
     3541 tacgccatct ttcaactcaa ccacgactta aacgcagaag tggaaacggt gtttctgctg
     3601 gccgcaaaag aatactcctt tgtttcaagc accctcatca aagaagtggc gcgttacgga
     3661 agaagtgtag aacaacacgc acctcctgca gtcagccggg cactccttcg caagttcggt
     3721 catgcaaaat agaaatctgg acagcgttct cgagatccct gcacccgagt tcattgtatt
     3781 tcgcgcgcaa acggcgggcc cgatggcacg cttcatggcc gtgtttctcg atcacgccat
     3841 catcttcctt ctgcttggaa tcactgcatt tttcgcggcc atatttgcag ccttcgagct
     3901 tgcaggaaca aagagcgccg gattttccac atttcttttg ctgattgcac caatttcata
     3961 gaggagttca ggcgttacgc tcgaaccttg aaatttgtct gcggcagcta attcttgaat
     4021 tgtgccaaga ttaagcacct gatattctac tcggaatgga ttactaaagc caaattttgg
     4081 taaacggcgg ctcagtggca tttgaccacc ttcaaaacca cgcttgcttt cgtagcccga
     4141 tgtcgccatg tgacctttat ggccgcgtgt agaggtacca ccgtgtccgg atcctgctcc
     4201 acgtccaata cgctttttct taccgataga ccctgatgca gggcttaaat ttccaatatg
     4261 cttcatagta attcctaatg ttatgcagtt atatcttcaa cggaaacaag gtgatttacg
     4321 acggcaatca tacccaatat ctgggtgttc ttcggctgta ttactgaata attcggtctg
     4381 ccgaggccca acgcttttat cgtacgtttt tgcttctcaa gacatttaat aatactgcgt
     4441 gtctgtgtaa ttttaatttt ttccatagct gtaagtagta gtaataatta tccgcgcatc
     4501 actttttcta ctgatatccc gcgtctcgct gatacagatt gtgcatcttc gagctgggtt
     4561 aatgctttaa tagtcgcttt gacagtatta tgaggattgg acgatcccat actttttgta
     4621 agcacgtcct gcacccctgc taattcaagt accgcacgta caccaccggc agcaataaca
     4681 cctgtaccag gggtagccgg cttgataaat acacgggctg caccgaactt tccgaccacg
     4741 tcgtgaggaa tagttgtatc ctgtaaacgg actgatacga catttttctt agctgcatcc
     4801 gttgcttttg taacagcatc aaccacttcg ccggctttac ctaagccata accgatgtga
     4861 ccattaccat cacctacgac gacaatcgca gagaaattga aacgacggcc accttttaca
     4921 acttttgccg tacgtcccac atagacgagt ttatctttaa gatcaatctc agaaactcta
     4981 atttttgcca ttatttttcc ttgttagatt agaattgcaa cccagcttca cgggccgcat
     5041 ccgcaagagc ctttacacga ccatgataaa tataaccgtt acggtcaaac gcgaccgatg
     5101 tgatgttttt ttcgagtgca cgcttcgcaa ttaatgcacc tacaatttta ctgctgtcgc
     5161 tcttcgtagt gtcattactc atagcagcaa tagcttcttt ttctacggtg caagctgaaa
     5221 ctaatgtcga gttttgtgta tcgtctataa tttgcgcata gatgtgcttt aagcttctat
     5281 acacagtcat ccgatagcgt tcaggtgacc cgctaatttt cttccgtaca cgcattt
//
LOCUS       DAORFF010000241         5306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8632_length_5306_cov_3.103980, whole genome shotgun sequence.
ACCESSION   DAORFF010000241 DAORFF010000000
VERSION     DAORFF010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8632_length_5306_cov_3.103980"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PKK76_10570"
     CDS             complement(<1..943)
                     /locus_tag="PKK76_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68961.1"
                     /translation="MKKITFRDRMRYRFDNLMSRGSIALIIYLGLLTVALIVGVTIID
                     IAGDLAPADDKGEKPGFSELLWKSLLRTLDPGTMGGDTGDWGFLFSMFVVTIGGIFIV
                     STLVGILATGIDQKLEDLRKGKSLVVEKDHTLILGWSENIYTIISEICEANRSRGKSC
                     IVILADKDKVEMDDEISSRIENPGKTRIVTRSGNPIDFVNLEIANPHSSRSIVVLSGD
                     GADPDAQVIKTVLAIINNPGRRKEPYHVVAELRRPESVQICEMIGKDEVKTVLSPEVI
                     SRIMVQTCRQPGLSVVYTELLDFGGDEVYIHEEASMAG"
     gene            1055..1285
                     /locus_tag="PKK76_10575"
     CDS             1055..1285
                     /locus_tag="PKK76_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017213738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNL68962.1"
                     /translation="MADPKHRQPENAPGKYYVDDTCVPCHVCVDEAPGLIQYAADEGH
                     VFFARQPVTPEEIRAAENAMKGCPTEAIGNDG"
     gene            complement(1307..3007)
                     /gene="ilvB"
                     /locus_tag="PKK76_10580"
     CDS             complement(1307..3007)
                     /gene="ilvB"
                     /locus_tag="PKK76_10580"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004491189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNL68963.1"
                     /translation="MKKSGSKLLVELLQSKGVDLVFGYPGGAILPFYDELYHSKIKHI
                     LVRHEQGAMHMAEGYARATGRPGVVIVTSGPGATNTITGLCDAKLDSIPLMVITGQVA
                     TTAIGTDAFQEADIFGISIPVTKYNALIRSPDDLARTFEEAWHLCNTGRPGPVLLDFP
                     KDVQIKETEVLRAKMELAPRHTSRQEITGDVKKLAEALNKAERPLLYVGGGAINSGAS
                     KEIKALAEKAMAPVTTTLMGLGAFPGTHILSVGMLGMHGTATANKAVLECDFLLSLGA
                     RYDDRVAGQADDFARDAVRAHIDIDASEFNKRVSVDHLVCGDLKDVIQAILPYVEKKD
                     RSPWVANTESLKQKNPLAYEESEEVIKPQRILKRLFEVTKGRAIVSTDVGQHQMWAAQ
                     YYQFDEPNRWLTSGGLGTMGYGLPAAIGAKFAKPDDLVVCVTGDGSYQMCIQELATIR
                     QYNLGVKILLLNNNFLGMVRQWQELFYEERFAESEWDYNPDFVKLAEAYGIPGMRITS
                     PDEIDSGLEFFLKDNGPAFLEALIPADEKVFPMIAAGKSQRDMLQFEDLKRMMQARQK
                     "
     gene            3049..4104
                     /locus_tag="PKK76_10585"
     CDS             3049..4104
                     /locus_tag="PKK76_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68964.1"
                     /translation="MTTPKEIKDQAEFLKMIGSTDLERPEIVLISGDDVEAVDSALDR
                     MKHRVLSRHKDCLVPVFGNEPDDDNRFLTEIENVPLFSPFRLIVVRDAQAVFASILKQ
                     KDRKSDFAHFISSLPDRTWILVHYSGRATDDFLSLWGSHAIHLATKDLYPEQVIDYIR
                     NASRRAGLSLAEEAVLEIRDRIVHRPGAIDAACARIRDHLPAGERNASLDFVQEVLFP
                     YPGWNLSTLVDSLFAGDSARFFSEIAKRDPSDDYYRPMKAVLNRTDELRRARIALDHG
                     LSDKDVMMFTGHGNKHPYVQKKILQRLRYETERFSNERILSIYDALDSLFKSFRRGTD
                     RDQQEILFTEKVASIFF"
     gene            4139..5092
                     /locus_tag="PKK76_10590"
     CDS             4139..5092
                     /locus_tag="PKK76_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL68965.1"
                     /translation="MKVKFWGVRGSIGSPVKGHALRDKVRRILQYASPADILDDEAID
                     RFLKSLPFSLLQTYGGNTTCLEIRSDKNDLVIVDAGTGIRELGMKMLSEGFLQGKGEC
                     AMVFTHTHWDHIQGLPFFAPFYIPGNKFEVHAISENLEDRLKYQHSSRHFPVGFEEMR
                     ATRSFVQHSEAEEWPLYGMKVRTKGMRHPGNSYSYRFEESGKSIIFGSDAEFKLEDMD
                     RIDEYMDYFRDADVLIFDTQYTFEEQLQKIDWGHSSASIATDIALRSGVKKLVLFHHD
                     PSYDDEKLDEVYLRALRYREMMDKQSKLEIVMAYEGLEIAV"
     gene            5089..>5306
                     /locus_tag="PKK76_10595"
     CDS             5089..>5306
                     /locus_tag="PKK76_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68966.1"
                     /translation="MTLCSRLFLLLLVFVSTSTFADELADRIAAVPNPRTTGGWVSDP
                     SQVLATRTDAINHLISDHEKETTDEIAVV"
BASE COUNT         1305 a         1308 c         1303 g         1390 t
ORIGIN      
        1 ttcctgccat tgatgcttct tcgtgaatgt acacttcgtc tccaccgaag tccaatagct
       61 ccgtgtatac aacggaaagc ccgggctgtc tgcatgtctg gaccatgatt ctagagatga
      121 cttctggaga gagaacagtc ttgacttcgt ctttaccgat catttcgcag atctgcactg
      181 attcaggtct tcttagttcc gccaccacat gatagggttc tttccttctt cctggattgt
      241 tgatgatagc aagaactgtt ttgatgacct gagcgtccgg atctgctccg tctcccgaca
      301 aaactacaat gctacgagat gaatgaggat ttgcgatttc aagatttaca aagtcgattg
      361 gatttccgct tcgcgtaacg attctggttt tgccgggatt ttcaattcta gatgaaatct
      421 catcgtccat ttctactttg tctttgtctg ctagaatcac aatgcacgat ttgccgcgag
      481 agcgattggc ttcacagatc tcagatatga tggtgtaaat gttttcggac catccaagga
      541 tcagagtgtg atctttctca actaccagag acttaccttt gcgaagatct tccagctttt
      601 gatcaatgcc cgtagcaaga atgcccacca gcgtgcttac aataaaaatg ccaccaatgg
      661 tgactacgaa catggagaag agaaagcccc agtcccctgt gtctccgccc atggtgcccg
      721 ggtccagtgt gcgaagtagg ctcttccaca acagttcgct aaagcctggc ttttcgcctt
      781 tgtcatctgc cggggcaaga tctccggcga tatcaatgat ggtcacgcca acgatgagcg
      841 ccactgtgag caagcccagg tatatgatga gggcaatgga ccctctggac atgagattgt
      901 cgaaacggta gcgcattctg tcgcggaatg tgattttttt catctgtgta gtggacccct
      961 ctcggcagtc tctggcgcag gtttcttttc gcgcaatcta ttttggaaga atcaaagtat
     1021 ttgacaggcg tagggtaaaa agtgcgcttc caacatggct gatccaaagc acaggcagcc
     1081 ggaaaatgct ccgggcaaat attacgtgga tgatacctgc gttccctgtc acgtctgcgt
     1141 ggatgaagct cctggactca tccagtatgc agcggacgaa ggtcatgtgt tcttcgcacg
     1201 gcaacctgtt acgccggagg aaatccgcgc ggctgaaaac gcaatgaaag gctgcccaac
     1261 agaagcaatc ggaaacgatg gctgagtaaa atcctcagcc attccctcat ttctgtctgg
     1321 cctgcatcat gcgctttaag tcttcgaatt gaagcatatc gcgctggctt tttcctgccg
     1381 caatcatggg gaaaactttt tcgtctgcag gaatcaacgc ttccaggaat gcaggaccgt
     1441 tgtctttgag gaaaaactca aggcctgaat cgatctcatc cggggaagta attcgcatgc
     1501 caggaattcc gtatgcttcc gcaagcttga cgaaatctgg attgtaatcc cattcagatt
     1561 cagcgaatct ttcttcatag aagagttcct gccactgacg taccatcccc aggaaattgt
     1621 tgttcaacaa caaaatcttc acgcccaggt tgtactgtcg aatggtcgca agttcctgaa
     1681 tgcacatctg gtaggatcca tctcctgtta cgcaaacgac cagatcgtct ggctttgcga
     1741 acttggctcc aatggcggca ggcagtccat atcccatagt gcccaggcca ccgcttgtga
     1801 gccagcggtt tggttcatcg aattgatagt actgcgccgc ccacatctgg tgctggccta
     1861 cgtccgtaga aacgatggca cggcccttcg taacttcgaa gagccttttg agaatgcgct
     1921 ggggcttaat gacttcttcg ctttcttcat acgcgagggg attcttttgt ttgagtgatt
     1981 ctgtgttcgc aacccacgga ctgcgatcct tcttttctac gtagggcaga attgcctgaa
     2041 tcacgtcttt gagatctccg caaaccagat ggtccacaga aactctcttg ttgaattcag
     2101 aagcatcaat gtcgatgtga gcgcgcactg catcgcgagc gaaatcgtct gcctgacctg
     2161 cgactctatc atcataacga gcgccaagag acagaagaaa atcacattcg agcacagcct
     2221 tgtttgcagt tgcagtaccg tgcattccaa gcatgccaac agaaaggatg tgagttccag
     2281 gaaacgcgcc cagccccatc agagtagtgg tgacaggagc cattgctttt tctgcgagtg
     2341 ctttgatttc tttggatgct ccggagttga tcgcaccgcc gcccacataa agcagaggtc
     2401 gctccgcttt gttcagagcc tctgcgagct tttttacatc tccggtaatt tcctggcgac
     2461 tcgtgtgacg cggagcaagt tccatcttcg cgcgaagcac ttccgtttct ttgatttgaa
     2521 catctttggg gaaatcaagc agcacaggtc ctggtctgcc agtgttgcac agatgccagg
     2581 cttcttcgaa ggtcctggcc agatcgtctg gagagcgaat gagtgcgttg tattttgtga
     2641 ctggaatgga aatcccaaag atgtccgctt cctgaaatgc gtctgtgcca atggctgtgg
     2701 tggctacctg gccggtgatc accatgagtg gaatggaatc cagcttcgca tcgcagagtc
     2761 ctgtgattgt gttggtcgct ccgggtccgc ttgttacaat gacaactcca ggtctgcctg
     2821 tggctcttgc gtaaccttct gccatatgca tggcgccctg ttcgtgcctc accaggatgt
     2881 gtttgatttt agaatgatag agttcgtcat agaaaggaag gatggctcca cctggataac
     2941 cgaacaccag gtccacccct ttggattgta aaagctcaac gagcagtttg gatccgcttt
     3001 tcttcattgc aaataccgcc acctctataa attcgagaac gaccctccat gacaactcca
     3061 aaagagatta aggatcaggc cgaatttctc aagatgatcg gcagcacgga cctggaaagg
     3121 cctgaaattg tgctgatttc gggcgacgac gtggaagctg tggactccgc cctggacagg
     3181 atgaagcaca gagttctttc caggcataaa gactgtcttg tgcccgtgtt cggaaatgag
     3241 ccagatgacg acaatcggtt tctgacagaa atcgaaaacg ttccgctctt ctctccattc
     3301 cgtctcatcg tagtcagaga cgcccaggct gtgttcgctt ccatactgaa gcagaaggat
     3361 cgcaaatcag attttgctca tttcatttcg tctctgccgg atcgcacatg gattctcgtt
     3421 cattacagcg gtcgggccac agacgatttt ctgtctctgt ggggatcgca tgccatccac
     3481 ctcgcaacaa aggatctcta tccggaacag gtgatcgact acattcgaaa cgcatccagg
     3541 cgagcgggtc tgagccttgc ggaagaagca gtcttagaga tcagagacag aatcgtgcac
     3601 aggccaggag cgattgacgc tgcgtgcgcc cggattcgcg atcatttgcc cgccggggaa
     3661 agaaacgcat ctcttgattt tgttcaggaa gttttatttc cttatccagg ctggaatttg
     3721 agcactctgg tagattctct tttcgcggga gactccgcgc gttttttttc ggagattgca
     3781 aagagagatc cgtcagacga ctactatcga cccatgaaag cagtgctcaa cagaaccgat
     3841 gagctgagaa gagctcgcat tgccctggac catgggctca gcgacaaaga tgtgatgatg
     3901 tttaccggtc acggcaacaa acacccgtac gtgcagaaga agatcctgca acgtctgcgc
     3961 tatgaaacag aacgattttc aaatgagaga atcctgagta tttacgatgc gcttgattcc
     4021 ctttttaaga gtttccgccg gggaacggac agagaccagc aggaaattct ttttactgaa
     4081 aaagtggctt ctattttctt ctagacggca gcccctgccc gttttttatc ctgggaatat
     4141 gaaggtgaag ttctggggcg ttcgtggatc catcgggtcc cctgtaaaag gtcacgccct
     4201 gcgcgacaaa gtgcggagaa ttctgcagta tgcttctccg gcggatatcc tggacgacga
     4261 agcaattgat cgattcttga aatcacttcc attttctctt ctgcaaacct acggcggaaa
     4321 tacaacgtgc ctggaaattc gttcagataa aaacgatctg gtgatcgtgg atgcggggac
     4381 aggcattcgc gagcttggaa tgaaaatgct ttcagaaggt tttctccagg gcaaaggaga
     4441 atgtgcgatg gtctttacgc acactcactg ggatcacatt cagggacttc cgttcttcgc
     4501 acctttttat attccaggaa acaagttcga agtgcatgcc atttcagaga atctagagga
     4561 cagactcaaa taccagcatt ccagcagaca ctttccggtt ggattcgaag agatgagagc
     4621 cactcgttct tttgtgcagc attcggaagc agaggaatgg ccactttatg gaatgaaggt
     4681 tcgcacaaag ggaatgcgcc atcccggcaa tagctattcg tacaggtttg aagaaagcgg
     4741 taagtccatc atcttcggaa gcgatgcgga atttaagctc gaagacatgg atcgtattga
     4801 tgaatacatg gattactttc gggacgcgga tgttctcatt tttgatacac aatacacatt
     4861 cgaagaacag cttcagaaaa tcgactgggg gcatagttct gcgtccatcg caacagacat
     4921 cgctctgaga tctggagtga agaaactggt gctattccat cacgatccat cttacgatga
     4981 tgaaaagcta gatgaagtgt atcttcgcgc tcttcgctat cgcgaaatga tggacaaaca
     5041 atcaaaacta gaaatcgtaa tggcttacga aggacttgaa attgcggtat gactctttgc
     5101 tctagactgt tcctccttct acttgttttt gtttcgactt ctacttttgc tgacgagctc
     5161 gcagatcgga tcgctgccgt tcccaatccg cgcacaacag gtggatgggt ttcagatcct
     5221 tctcaggttc ttgcaacaag aacggacgcc atcaatcatc tgatctctga tcacgaaaaa
     5281 gaaaccacag acgaaatcgc agtcgt
//
LOCUS       DAORFF010000242         5299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8650_length_5299_cov_3.505721, whole genome shotgun sequence.
ACCESSION   DAORFF010000242 DAORFF010000000
VERSION     DAORFF010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8650_length_5299_cov_3.505721"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..1104
                     /locus_tag="PKK76_10600"
     CDS             43..1104
                     /locus_tag="PKK76_10600"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNL68967.1"
                     /translation="MAGQSKVMSPESKPNASIRFRGIGSYIPSRRITNQHWESLVSTS
                     DEWIQKNIGIRERSRIGPEETTTDMGVHSAKLAIETAGVDPASIDFILCATNSQDDLW
                     PSTAAKIQERLGIKTTSLDLQAGCSGWLFGVRLGTSLLGSGQAKRVLVVGSDALTRFL
                     NYYDRDNMLFGDGSGSCILDLDPSYTARRLPVPIFDTGTLPSRAMALETIYTEKENAM
                     EKYLAREDMSLANRPIPSMEGRTAMKYALTKTKESIASVLSQARAMGIEKEDIALYAP
                     HQTNALVVQSLCEFAGFPIEKIPYTLDKYGGISTAGIPTGLYEHYRAGKLKKGDLVLS
                     SAYGAGFTYGAMIFEWGVD"
     gene            complement(1101..1631)
                     /locus_tag="PKK76_10605"
     CDS             complement(1101..1631)
                     /locus_tag="PKK76_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HNL68968.1"
                     /translation="MDQYKEEKKRIRLVRADITKLSVDAIVNAANESLLGGGGVDGAI
                     HSAAGPDLLRACAALKGCKTGQAKITPGFQLPAKYVIHTVGPVYRGGMNKESELLASC
                     YQSSLAVASHNNVNTIAFPSISTGVYGYPIEEACRIAIVETLRYLSGHSEITEVTFVC
                     FSDRDLKIYERAMTEF"
     gene            1694..3388
                     /gene="gpmI"
                     /locus_tag="PKK76_10610"
     CDS             1694..3388
                     /gene="gpmI"
                     /locus_tag="PKK76_10610"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNL68969.1"
                     /translation="MKSGESKLEPLKRLEKSIPGPLVFVIMDGVALAKGQAQGYPGNA
                     LDLSNAPCIKGLLASAPVQTTLQAHGTAVGMPSDEDMGNSEVGHNAMGAGRIFSQGAS
                     LVNQAIANKSLFAGKVWKKLIGNASEPGAALKGSAVHFIGLLSDGNVHSHIDQLISLL
                     QECSAQKVKEVYVHPLLDGRDVAKASAGIYIDKLTEALSSLAPENGKWRIASGGGRQV
                     ITMDRYEANWSMVELGWKTHVKGEGRPFSSAQEAIATMRKELPDAIDQDLPPFVIFEN
                     GKPVGPIKSGDVVIYFNFRGDRAIEISRAFTEKDFKPFKRDPEVSVHYAGLMQYDGDL
                     NLPEDYLVSPPAIDHTISQYLVDAGVKQYACSETQKFGHVTYFWNGNNSQKFSETLEV
                     WEEIPSDRISFDFKPEMKAKEITDAALKALSTKEFGFLRVNYANGDMVGHTGNLPASI
                     RAVEYVDEAIARLLEGAKAAGATVLITADHGNCDEMIMLDKKGNAVKDEKGAYVPKTS
                     HTLNPVPFILTGTASENYEIAAGKPGLANLAATVLTLLGFQKPEMYLPSIVQLKSK"
     gene            complement(3459..4316)
                     /locus_tag="PKK76_10615"
     CDS             complement(3459..4316)
                     /locus_tag="PKK76_10615"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNL68970.1"
                     /translation="MISFGRMAETTFHIRAQDGTRLAGSAWVPRRPKQVIAWIHGFAE
                     HRKRYAHLANWMNEERVALVAIDVRGHGESDGKRGYVHLFSDYFWDVSALLHYVRENF
                     QKVPVSIGCHSHGGLIVSRYLEENPPPVDLKCAVFSAPFLGLPPDFPPWKRKLGDAVA
                     GLVPGLSVPTGIDAKYISHDTKIQALYAGDPTIFKTATVRWFSEVLNAQAAALSRSPR
                     IRLPAIVLQGMADRIVSVDASRRFYEDLPSDKKKWVPYRGLYHEILNETNRLEVYKEI
                     YKYIKKFGR"
     gene            4325..>5299
                     /locus_tag="PKK76_10620"
     CDS             4325..>5299
                     /locus_tag="PKK76_10620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNL68971.1"
                     /translation="MTSKLRVAVVHDWLTGMRGGEAVLESLLALYPHADLFTLLHNQG
                     SVSKFVEERKIRTSFIDRLPFKSRKYRNYLPFFPTAIETFSFRGYDLVISSSHCVAKG
                     VIVPPGTPHICFVHSPMRYVWDMTQDYFPYEFEDGSPGSLFNRFVFPFFANYLRMWDS
                     ASAHRVDRYIANSSFVAERIRKYYGRSAGVVHPPCIEEIPRSLPKERSDFYLIVSALV
                     PYKRVDLAIDAFRNLNRKLIIVGDGPDLKRLKSMAGPEIEFHGKVSREEIDRLYSQAR
                     ALVFPGIEDFGIVPVEAQAKGCPVIAYGRGGALETVTEKTGVFFHEQSP"
BASE COUNT         1414 a         1355 c         1297 g         1233 t
ORIGIN      
        1 acatccattg gacgctcggg aatcatagcc tgaagttccg gcatggccgg ccaatctaaa
       61 gttatgtcgc ctgaaagcaa gccaaatgca agcattagat tcagaggaat cggctcttac
      121 atcccttctc gcagaatcac caaccagcac tgggaaagcc tcgtttccac gtcagacgaa
      181 tggatccaaa agaacatagg aatccgcgaa agatctagaa tcgggccgga agagactaca
      241 acggacatgg gagtgcattc tgcaaagctc gccattgaga cggcgggtgt agatcctgcg
      301 agcattgact tcattctatg cgctacgaat tctcaggacg atctgtggcc atccaccgcg
      361 gccaagattc aggaaagact cgggatcaaa accacatccc tggaccttca ggcaggatgc
      421 tctggctggc tctttggagt tcgactagga acgagtctgc ttggttccgg ccaggccaaa
      481 agagtgctcg tagtcgggtc agacgcgctc acccggtttc tgaactatta cgatcgcgac
      541 aacatgcttt ttggagatgg aagcggctct tgcattctgg acctggaccc atcgtatact
      601 gcaagaagac tgcccgttcc tatctttgat acaggcaccc tgccttcgcg cgcgatggct
      661 cttgaaacca tctacactga aaaagaaaac gcgatggaga agtatcttgc gcgcgaagac
      721 atgtctctgg ccaatcgacc cattccttct atggaaggtc gaactgccat gaaatacgcg
      781 ctcacgaaaa ctaaagaatc tatagcatct gtcctatctc aggcgcgcgc tatgggcatt
      841 gaaaaagaag acatcgcact ctacgcgcca caccagacga atgcacttgt ggtgcagtct
      901 ttgtgcgaat ttgctggatt tccaatagaa aagattcctt acactctgga caaatacggt
      961 gggatctcta cagccggaat tccaaccgga ctctacgaac actatcgcgc cggaaaatta
     1021 aaaaaaggcg atctggttct gtcctctgcc tacggtgcag gcttcaccta tggtgccatg
     1081 atatttgaat ggggtgtgga ctaaaactca gtcatcgctc gttcgtagat cttcaaatcc
     1141 ctgtcggaga aacaaacaaa ggtaacttcc gtaatctcag agtggccgga caagtagcgc
     1201 aaagtctcca caatggcgat tctgcatgcc tcctcgattg gatatccata aactccggtg
     1261 ctgatagagg gaaatgcaat cgtattcacg ttgttatgcg atgcaaccgc aagagaagat
     1321 tggtaacagg atgcaagcag ctcgctttct ttgttcatcc ctcccctgta cactggtcct
     1381 actgtgtgga tcacatattt tgccggaagc tggaagccgg gagtgatctt tgcctggccg
     1441 gtcttgcatc ctttgagtgc agcacaggcc cgcagcagat ctggcccggc agcactgtgg
     1501 atcgcaccgt ccacaccacc tccacccagc aaagattcgt tggctgcatt tacaattgca
     1561 tccacagaaa gctttgtgat gtccgctcgg accaaacgga ttcttttttt ctcttcttta
     1621 tactggtcca tctgcactct cccgccgggt cgtttttcct tgacgcgtac ttcactgtct
     1681 ccagaaatca agaatgaaat ccggcgaaag caaacttgaa ccactcaaac gcctcgaaaa
     1741 atccatcccg ggcccgctgg tatttgtaat catggacgga gtggctctgg caaaaggcca
     1801 ggcgcaaggt taccccggaa acgcactcga tttgagcaac gcaccgtgca tcaagggact
     1861 actcgccagc gcgcccgtgc aaaccactct tcaggctcac ggaactgcag taggaatgcc
     1921 ttcggatgaa gacatgggaa acagcgaagt gggtcacaac gccatgggtg ccggtagaat
     1981 ctttagccag ggagcaagcc ttgtaaatca ggcaatcgca aacaagagcc tgtttgccgg
     2041 aaaagtctgg aagaaattga tcggaaatgc aagcgaaccc ggagctgcgc tcaaaggaag
     2101 cgcggtccat ttcattggcc ttttgtcaga cggaaacgta cacagtcata tagatcagtt
     2161 gatcagctta ctacaagaat gctccgcgca gaaagttaag gaagtttatg ttcatcctct
     2221 tctagacgga cgcgatgttg ccaaagcgag tgccggaatc tacatagaca aactcactga
     2281 agcactctct tctctggctc ctgaaaacgg aaaatggagg atcgcatcgg ggggcggacg
     2341 tcaggtgatc accatggatc gatacgaagc gaactggagc atggtggaac ttggatggaa
     2401 gactcatgtc aaaggagaag gcagaccatt cagctccgcc caggaagcaa ttgcaaccat
     2461 gcgaaaggaa ttgccggatg ccattgacca ggatctacca ccgtttgtaa tttttgaaaa
     2521 cggaaagccg gttggtccaa tcaagtccgg agatgtggtc atctacttca actttcgcgg
     2581 agaccgtgcc attgaaattt cgcgcgcctt cacagaaaaa gatttcaagc ctttcaagcg
     2641 cgatcctgaa gtttctgtac actacgccgg cctgatgcag tacgatgggg atctaaatct
     2701 accagaagat tatctggttt ccccgccggc catcgatcac accatttcac agtacctggt
     2761 ggatgcggga gtgaagcagt atgcctgctc tgaaactcag aaatttggcc atgtaaccta
     2821 cttctggaac ggaaacaatt ctcagaaatt ttccgagaca ctcgaagtct gggaagaaat
     2881 cccatcggat cgaatttcgt ttgattttaa gccagaaatg aaagccaaag aaatcacgga
     2941 cgcggcgctc aaggctctct ccacgaaaga atttggattc ttgagagtga actacgcaaa
     3001 tggagatatg gtaggccaca ctggaaatct tcctgccagc attcgcgcag tggagtatgt
     3061 ggatgaggcc atcgcacgac tgctcgaagg tgcaaaagca gcaggagcaa ccgtgctcat
     3121 tacggcggat cacggcaact gcgatgagat gatcatgctg gataagaaag gaaacgctgt
     3181 gaaagatgaa aaaggcgctt acgttcccaa gacaagccac actctcaatc cggttccctt
     3241 tattctgacc gggactgcgt cagagaatta tgaaattgct gctggaaaac cgggtctcgc
     3301 gaatctggcg gccacagtac tcacgctgct tggatttcag aaaccggaga tgtatcttcc
     3361 gtccatcgtc caattaaaat caaaatgatc cagcaccact ggccaggcac agaaagaccg
     3421 gtggcaaatc acattggcat tcggattgtg ccctgatttc atcttccaaa ttttttaata
     3481 tatttataaa tttccttata tacttcaaga cgattagttt cgttcagaat ttcgtgatag
     3541 agcccgcgat agggaaccca tttcttttta tcagaaggaa gatcttcata gaagcgtctg
     3601 gatgcgtcca cagatacaat cctgtcagcc atgccctgaa gcacaatggc cggcaggcga
     3661 atgcgcggtg atctagaaag agcggctgcc tgtgcattca acacttcact gaaccatcga
     3721 acggtggctg ttttgaaaat cgttggatct cctgcataga gagcctggat ctttgtatca
     3781 tgcgaaatgt atttagcatc aattccggtt ggaacggata gccctggaac aagaccggcc
     3841 actgcatctc ctagctttct tttccagggc gggaaatcgg gaggaagtcc aaggaacgga
     3901 gcgctgaaca ctgcacactt caaatccaca ggaggtgggt tttcctccaa atacctggat
     3961 acaatcaaac ctccgtgcga atgacatcca atggatacag gaactttttg aaagttttct
     4021 cgaacgtagt gcagtagagc agatacgtcc cagaagtaat ctgaaaacag atgcacataa
     4081 ccgcgctttc cgtctgattc cccatgcccg cgcacgtcga tcgccaccag ggcaactcgc
     4141 tcttcgttca tccagttggc caagtgagca tatcgcttgc gatgctccgc aaagccatgg
     4201 atccaggcga tcacctgttt gggtctgcgt gggacccacg cgctgccagc cagccgggtg
     4261 ccatcctgcg cacggatatg aaatgtggtt tccgccatac gtccgaatga aatcatcttc
     4321 ctgaatgaca agcaaacttc gcgtcgcagt agtacatgac tggctgacag gaatgcgcgg
     4381 gggggaagca gtgctcgaat cgctgcttgc actttatccg cacgcagatc tttttactct
     4441 gcttcacaac caaggttctg tcagtaaatt cgtagaagaa agaaagattc gcaccagttt
     4501 catagaccgc ctcccattca aatccagaaa gtatcgcaat tatctgcctt tttttccaac
     4561 agccattgag acgtttagct ttcgcggcta cgacctggtc atatccagct ctcactgcgt
     4621 tgcgaaagga gtgattgttc cacctgggac tcctcacatc tgcttcgtac acagtcccat
     4681 gcgctacgtc tgggacatga cccaggacta cttcccctac gagttcgaag atggctctcc
     4741 tggaagtctg ttcaatcgat ttgtgtttcc gttctttgcg aactacctga gaatgtggga
     4801 ttcagcaagt gcgcatcgcg tggacagata catcgcaaac agcagctttg tcgcagaaag
     4861 aattcgtaag tactacggaa gatctgctgg agtcgttcac ccgccatgca ttgaagaaat
     4921 cccgcgatca ctgccaaaag aaaggtccga cttctatctg atcgtaagtg cactcgttcc
     4981 ttacaaaaga gtagatctgg ccattgatgc atttcgaaat ctaaacagga agctgatcat
     5041 tgtaggagac ggcccggatc taaaaagact caaatccatg gcaggtccgg aaatcgagtt
     5101 tcacggcaaa gtctcgagag aagaaattga tcgcctctat tcgcaggcgc gagcacttgt
     5161 atttccaggc atcgaggact ttggcattgt gccggtagaa gcgcaggcaa agggctgccc
     5221 cgtcatcgcc tacggacgcg gaggtgctct tgagactgtc actgaaaaaa caggtgtgtt
     5281 cttccacgag caatcccct
//
LOCUS       DAORFF010000243         5285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8685_length_5285_cov_3.773614, whole genome shotgun sequence.
ACCESSION   DAORFF010000243 DAORFF010000000
VERSION     DAORFF010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8685_length_5285_cov_3.773614"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PKK76_10625"
     CDS             <1..715
                     /locus_tag="PKK76_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNL68972.1"
                     /translation="FGAYAVDRERTRNFLYLECLSSYACLMLESGIPSLVYPEGTRSR
                     DGKIVGIKTGLLSTAVSAYQNTGREVVVMPLALSYESIPEDSFYCGLGEEPGLKEFIN
                     KRGSAYLDICDPIRVSRHMSADDPTLSISMAIVEQWKKHLRILPNQILARILSDNGHA
                     VPRAGLPALVEEFVLRNPGNYLTRDAEAIVEQGIASLRERNIAKEDGVQLASVTPALA
                     SYYGGMVPESGGTILDQDA"
     gene            complement(994..2277)
                     /gene="xseA"
                     /locus_tag="PKK76_10630"
     CDS             complement(994..2277)
                     /gene="xseA"
                     /locus_tag="PKK76_10630"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNL68973.1"
                     /translation="MDAPARNIFSVSRVNSLIRDILQNELHLQNIWMEGEITNLVHHT
                     SGHIYFSLKDEQSTISCTFFKYANQKVREIKLANGMRIQARGGISVFAPRGSYQFNVQ
                     QVLPAGEGLLRLQIEQLKKKLHAEGLFDLSRKRPLPPFPFTLGVASAPTGAAIQDIIR
                     VARSRFPGINILLAPCVVQGPGAVESIVYAIQLLNKPEFNVDVIIAGRGGGSFENLLP
                     FNDERVARAFAGSRVPIVSAVGHEIDHPISDLAADDFAPTPSAAVLKVLPLMSEITER
                     IEECALRLRLSLVHSHTRGRRRLLQILNSKIHKKPAAMLEIWQMRMDQMHRSFNLGMQ
                     SRLHSNSRRLEKFDHFGMHFKAGLKRNEERFKLLEERLTNFSPLATLRRGYSLVRDKD
                     KKVIRSAAQVERGDEIEIMLSQGRLKAKVTEALKE"
     gene            complement(2280..3383)
                     /locus_tag="PKK76_10635"
     CDS             complement(2280..3383)
                     /locus_tag="PKK76_10635"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNL68974.1"
                     /translation="MDRPGFLNRETAFPYLSIVLMVGSSLLLVWLFSSFVWPVFLALV
                     LYVAFEGIHKKFQSITGKYKGLAATLSMLFVMIIIFGPILLLIRLLVLEAIDVTFRLK
                     DILASDDVLKMVPLLPSFADPLTSDPFFWVDLQDTVLHVVDRYTSFLDPDKLGSLLGN
                     AGSFVFGSMAVTVTIVVNLLFALILLFFLFRDGRVFYEFIEEVLPIPQDRLKIFVERL
                     GESLRAVVRGNLFISILQGCALGLGVYFCGFSRGLLFGAVAAIFSLIPVVGTAVVWLP
                     AALYLGFVENSYGLAAFMAIYGPAVYLVLENIVKPKILDRQLGIHPIFLFFAILGGLA
                     EFGITGVIIGPIMVTVFVTLWSIMPRRAPASAT"
     gene            complement(3385..3837)
                     /locus_tag="PKK76_10640"
     CDS             complement(3385..3837)
                     /locus_tag="PKK76_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68975.1"
                     /translation="MKRATLRIAFIIASMSFLSAGCGDNTSQRVVRFKDFRITQNPTD
                     LPAGLPAGFRYPDARAVLSAGYEPESYASSQEGSIEFESRDDVQHIEAYYNNAFREQG
                     WKVIQSRSEEKALLLMAESAYSEVVTVIARSGPPSRIRVYMRRLGRDG"
     gene            complement(3834..>5285)
                     /locus_tag="PKK76_10645"
     CDS             complement(3834..>5285)
                     /locus_tag="PKK76_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HNL68976.1"
                     /translation="SLPDNIRKVIDGLSELERELTLECFQQGGILEMVRAKEIVTARK
                     GKWEDPIQKLNHLGILIDDYYIDEKFVRLLIMPVEVFALYTENPALPGEKKGTRKRQE
                     RIITNDLDFALNIKKMIVYLGRKGLNLAKSGKIKQIDLKDTENKFLRPDIALFMEKSQ
                     IYQIELLLPLMRQMDIVRVKRDDVVLRNDYESILEMDPFDLQKRIMNEITAGRERRVR
                     YEDVFEPMYVPFYDKAVFDECVSAVQKRGRVLYFVILATMIREKLILSKTFRVKNFQQ
                     ELADLRKEVTSAFFYLQLMGLINVDYPDRKIEVSELGNNIFRGKSLVRDDQKGGIILN
                     PDMSLIAIPEKLSIRGLYLLKCFLELKSFDNVYTFQLTRDSFLEGLLLKRNPQELIDF
                     LRTTCRSDLPQNLLFSIEEWSRSVPLVTITDECVLVQTQDASHMDLLLGQISGRKFVT
                     QKISPTSILIESDKIAETIQISEKLNLIVRLIR"
BASE COUNT         1336 a         1247 c         1356 g         1346 t
ORIGIN      
        1 ttttggagcc tacgccgtcg atcgggaacg gacgcgaaat tttctctatc tcgaatgtct
       61 ttcgtcttac gcttgtttga tgcttgagtc tggcattcca tccctggttt atccggaagg
      121 aacgcgaagt cgcgacggaa aaatcgttgg aatcaagaca ggtcttttgt ctactgctgt
      181 ctcagcctat cagaacacgg gccgcgaagt cgttgtaatg ccccttgctc tttcctatga
      241 aagcatcccc gaagattcat tctattgtgg actcggcgaa gaacctggcc tgaaggaatt
      301 catcaacaaa agaggaagtg catacctgga catttgtgat ccaattcgag tgtccaggca
      361 catgtctgcg gatgatccaa ctctctccat atccatggcg attgtagaac agtggaagaa
      421 gcatttgcgt atcttaccca accagattct cgcacgcatt ctatctgaca atggccacgc
      481 ggtgccacgt gccggcctcc ccgctcttgt ggaagagttc gtgctgcgaa atccagggaa
      541 ctatctgacg cgcgatgcgg aggcgattgt ggagcaaggc attgcatcgc tccgggagcg
      601 caatattgcg aaggaagatg gtgttcagct ggcatctgtt acgcccgctc tcgcctcgta
      661 ttacggcggt atggtgccgg aatccggagg aacgatcctg gaccaggacg cgtgattttt
      721 tggccacatt gattcgcaaa agtacttgca atcttttaca attcgctgac actgtgtcag
      781 ggaattcgct ttcccagggg gactcaaatg aaacagcttt taggtgtcgt ggctatggcc
      841 aggtcctcgc agtgacaatt tcaagaattt gcataagacg cagtcttata aggcaagcga
      901 tttcacatgt gctgcctttg aagacgcaga gctgcttgca aaagcaaaac tcttggagca
      961 agaggcggcg gaagaaaagc aatagaaggg tggttattct ttcaaggctt ccgttacttt
     1021 cgctttcagt ctgccctgag agagcatgat ttcaatttca tcgccgcgtt ccacctgcgc
     1081 ggcggatcga atgacttttt tgtctttgtc ccgaaccaga gaatagccgc ggcgcaatgt
     1141 ggccagagga ctgaagttgg tcagtctctc ttccagtaat ttgaatcgtt cttcgtttcg
     1201 cttcagtccg gctttgaagt gcatcccgaa gtgatcaaat ttttccagtc tgcgactgtt
     1261 gctgtgcaat ctgctttgca tacccaggtt gaacgatcga tgcatctgat ccattctcat
     1321 ttgccagatc tcaagcatgg ccgccggttt tttgtggatt ttggagttga gaatctggag
     1381 caatcttctt cttccgcgtg tatgcgaatg cacgagggaa agcctcaatc tcagagcgca
     1441 ttcttctatg cgttctgtga tttctgacat gagcggaaga accttgagaa ctgcagcgct
     1501 gggcgttggc gcaaaatcat ctgctgcgag atcggatatt ggatgatcga tttcatgtcc
     1561 tactgcggat acgatgggca ctcgcgaccc ggcaaatgct cgtgccactc tttcgtcgtt
     1621 gaagggcaaa aggttttcaa aggatccacc gcctctgcct gcgatgatca catctacgtt
     1681 gaactccggt ttattcaata gttgaatcgc atatacgatg gattccacag cgcccgggcc
     1741 ctgcaccaca cagggcgcaa gcaagatgtt gattcccgga aaccgagatc gcgcaacgcg
     1801 aatgatgtcc tggatggctg cccctgttgg agcagaagca actcccagtg taaaaggaaa
     1861 aggaggcagc ggcctttttc tcgacagatc gaacaagccc tccgcatgca gttttttctt
     1921 caactgttcg atttgaagac gaagcaaacc ttcgcctgca ggaagtactt gctgcacgtt
     1981 gaattgataa gaaccgcgag gagcgaatac agaaattcca ccgcgtgcct ggattcgcat
     2041 tccattggcc aatttgattt cgcgcacttt ctgattcgcg tacttgaaaa atgtgcagct
     2101 aatggtggac tgttcgtctt ttagagaaaa gtaaatgtga ccacttgtgt gatggaccag
     2161 gtttgtgatt tcgccttcca tccagatgtt ctgcaggtga agctcattct gcagtatgtc
     2221 ccgaatgaga gaatttacgc gcgaaacgct gaatatgttt cgagcaggtg cgtccatgtt
     2281 taagtcgctg aggcgggagc gcgtctgggc atgatgctcc agagcgtcac aaatacagtg
     2341 accatgatag gtcctatgat cacaccggta attccaaact cagcaagtcc gccaaggatg
     2401 gcgaaaaaga gaaagatggg atggatgcca agctgacgat ccaggatttt tggcttcaca
     2461 atgttttcga gaacgaggta aacagcaggg ccgtagatgg ccatgaacgc tgcgaggccg
     2521 taagaatttt ctacaaaacc aaggtagaga gccgccggga gccatactac agcagttccg
     2581 accactggga tgagagagaa gatggctgct acggcgccga ataaaagtcc gcgcgaaaat
     2641 ccgcagaaat aaacaccgag tccaagggcg catccctgca agattgagat gaacaaattc
     2701 ccgcgaacga cggcgcgaag cgattcgcca aggcgttcta cgaaaatctt gagtctatcc
     2761 tgcgggatgg gcaggacttc ctcaatgaat tcgtagaaaa ctcttccatc tcgaaataga
     2821 aaaaacaaaa gtatgagcgc aaacagtaga ttcactacga tcgttacagt tacggccatg
     2881 cttccgaaaa caaaactgcc tgcgttgcca aggaggctgc ccagtttgtc cggatccaaa
     2941 aagctagtgt aacgatccac tacatggagc actgtgtcct gcaaatcaac ccagaagaaa
     3001 ggatccgatg tgagtggatc ggcgaaggaa ggaagtagcg gaaccatctt cagtacatca
     3061 tctgaagcca gaatgtcttt caggcgaaac gtaacatcaa tcgcttccag gacaagcagt
     3121 cgaatcaaaa gcaagatggg gccaaagatg atgatcatca caaagagcat gctgagagtg
     3181 gccgcaagcc ctttgtattt tcccgtgatg ctttggaact tcttgtgaat tccttcaaag
     3241 gccacgtaga gaacgagcgc gagaaagacc ggccagacaa aggaactgaa taaccagaca
     3301 agaaggaggg acgagcctac catcagcaca atggatagat agggaaatgc agtctctcga
     3361 ttgagaaaac cgggccgatc catgttagcc gtctctaccc aggcgtctca tgtagactcg
     3421 aattctggaa ggtgggccgg atcttgcgat cacggtgacc acttcgctgt aagcggactc
     3481 tgccatgaga agcaatgcct tttcttctga cctgctctgg attactttcc agccctgctc
     3541 gcgaaatgcg ttgttgtaat acgcttcaat gtgctgcacg tcatcgcggc tttcaaattc
     3601 aatgctgcct tcctgggaag atgcatagga ttctggttca taccccgcag acagtacagc
     3661 gcgtgcgtca ggatatcgaa accccgctgg aagtccggct ggaagatctg tcgggttctg
     3721 tgtgattcga aaatctttaa agcgaacgac tctctgtgaa gtgttgtcac cgcatcctgc
     3781 ggacaaaaat gacatgctcg cgatgatgaa cgcgatccgt agagtggcgc gtttcaccgg
     3841 atgagtctca cgatcagatt cagtttctca gagatttgaa tcgtctctgc aattttgtca
     3901 ctttcgatga gtatggaagt gggggatatc ttttgagtga cgaacttgcg gcctgagatt
     3961 tgcccgagca gaagatccat gtgtgacgca tcctgagttt ggaccagtac acattcgtcc
     4021 gtgatggtca caagaggaac agaccgagac cattcttcaa tggagaatag gaggttttgc
     4081 ggcagatcgc tgcgacaggt agtgcgaagg aaatcaatga gctcctgagg attgcgcttg
     4141 aggagcaagc cctcgaggaa ggagtcgcgt gtaagctgga acgtgtatac attgtcaaag
     4201 ctcttgagtt cgaggaaaca cttcaataga tagaggccgc gaatagagag tttttccggg
     4261 attgcaatca gactcatgtc tggattgaga atgataccac ctttttgatc atcgcgcacg
     4321 aggctcttgc cgcggaagat gttgttgcct agttcagaaa cctcgatctt gcgatccggg
     4381 tagtctacat tgatgaggcc catcaattga agatagaaga atgcgcttgt cacttctttg
     4441 cgaagatctg ccagttcttg ttgaaagttc ttcacgcgaa aagttttcga gagaattagc
     4501 ttttcgcgaa tcatagttgc caggattacg aagtaaagta cgcgtccgcg tttctgaact
     4561 gcagaaacgc attcgtcgaa cactgccttg tcatagaaag gaacgtacat tggttcaaac
     4621 acatcctcgt agcgaacgcg tctttctctg ccggctgtga tctcgttcat gattcttttt
     4681 tggagatcga acggatccat ttccagaatg gattcgtaat cgttgcgaag caccacatcg
     4741 tcgcgcttga cgcggacaat gtccatctgg cgcatcaaag gcagcagcaa ttcaatttga
     4801 tagatctggc tcttttccat gaaaagagca atgtccgggc gaaggaattt gttttctgta
     4861 tcttttagat cgatttgttt gatcttgcct gactttgcca ggttcaatcc tttgcgtccc
     4921 aaatacacga tcatcttctt gatgttcagt gcgaaatcaa gatcattggt aatgatgcgt
     4981 tcctggcgct tgcgtgttcc tttcttttca ccgggcagtg cagggttctc cgtgtagagt
     5041 gcgaagactt ccacgggcat aatcagcaat cgcacgaact tttcatcaat gtaatagtca
     5101 tctatgagaa tgcccaggtg gttcagtttc tggattgggt cttcccattt tcctttgcgc
     5161 gccgtgacga tttctttagc gcgcaccatt tcaaggatgc cgccttgttg aaagcactcg
     5221 agtgtaagtt cgcgttccag ttcggaaaga ccatcgatca ccttacggat gttatccgga
     5281 agact
//
LOCUS       DAORFF010000244         5254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8765_length_5254_cov_3.884209, whole genome shotgun sequence.
ACCESSION   DAORFF010000244 DAORFF010000000
VERSION     DAORFF010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8765_length_5254_cov_3.884209"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PKK76_10650"
     CDS             complement(<1..662)
                     /locus_tag="PKK76_10650"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNL68977.1"
                     /translation="MPESSKHAIPEAVRARILDWAGPSFPLEIREEAVRVHADIMAGK
                     HSEEVEAYSGELEFGTGGLRGVLGHGAGRMNRYTVGRATLALCRVFAKRSRKPALVIA
                     YDSRRKSDEFSIVTAGIAAAQGFKVYLFDDVAPTPILSYAVRRLQAASGVVITASHNP
                     PEYNGYKVYAEDGSQIVGSIQKEIEKEIAAIAWSDLKFLSESDPLFKKQVKKIGPEIR
                     KA"
     gene            748..1074
                     /locus_tag="PKK76_10655"
     CDS             748..1074
                     /locus_tag="PKK76_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL68978.1"
                     /translation="MITAEPRNNTLILHVTEPRVDMVVARKFREGLTAYLKDKPHRIV
                     LDLKETEYFDSSALGALVAFMKDVKAYGGQLVLCNLSRSLLTLLKLSKLDLLFEISED
                     LEKSIV"
     gene            1123..2394
                     /locus_tag="PKK76_10660"
     CDS             1123..2394
                     /locus_tag="PKK76_10660"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HNL68979.1"
                     /translation="MEESATSVIIEQLQKLSGSRREDFTEDTIIGSDINQIRHTFYFL
                     RSERIPVIISYMGETYQARVERVENDRIILNAPGFSEGTARRCRLKFEIINILYQFEV
                     PIVDLSRDKIVVRMPAFVQSARHRKYVRVYPQDLFMRFNLVYMPMFGRRGVGQIVENR
                     YPKIVRELERDRPDLGLLVRIVTEAINEISPDYEFVMYNPDVKRTFLERVMMSEKKTL
                     FVRDTSRQDTYYSPLGIFGITNFEKEFRRIAREESIEEAENFFEQYRRAELVDFIANY
                     ICTPIILLDRVVGHIKIYSTVLDKRNLNPDEAQRIDLLVQLLNYALSKHAIAETYFTL
                     HNTKVINISMSGLLFELQDSNVFNYLLDHDRLKICIPIKAHEVELRAEITRLLPAENG
                     YRIGVMFFSGAPDDFHFLEGFIHDRIKAMLR"
     gene            complement(2450..3898)
                     /locus_tag="PKK76_10665"
     CDS             complement(2450..3898)
                     /locus_tag="PKK76_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNL68980.1"
                     /translation="MGKPTGFMEFARASLKVEAPADRIKHYKEFEKTPDAETAQSQGA
                     RCMDCGIPFCHGEYGCPTDNLIPEFNDLIYKGRWRDALENLHSTNNFPEFTGRLCPAP
                     CESACVLGIIEPAVTIKYNERTIIDRGFSEGWVEPQAPQKLLGTTVAVVGSGPSGLAC
                     AQQLARAGHKVTVFEKNDRAGGLLRYGIPDFKMEKWLIDRRLEQMRIEGVTFKLGVNV
                     GVDISAEELIATFDAVVLALGCEVPRDLPLPGRQLKGIHFAMDFLRRQNKINAGDQVP
                     DAISAKGKNVIVIGGGDTGSDCVGTSNRHGAKSVRQIEIFDEPPKTRPSSTPWPTYPR
                     ILRTSSSHEEGCIREWAVSTKEFKGNANGEVATLHGCKAKFEGGKFVDIPGSEFVWEA
                     DLVLLAMGFVNPVQDGLIAQLTKMGMALDERKNVKATFGLEDGTFATTLPNVYACGDV
                     RRGQSLIVWAISEGRKCAAQVHRNLLAKLKTV"
     gene            complement(3909..>5254)
                     /locus_tag="PKK76_10670"
     CDS             complement(3909..>5254)
                     /locus_tag="PKK76_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNL68981.1"
                     /translation="GTPWELGLAETHQTLVANGLRDRVVLATDGKLLTGRDVVIGALL
                     GAEEFGFSTSALVSVGCIMMRKCHLNTCPVGVATQDEELRKKFHGKPENVINLMTFIA
                     QEVREIMAQLGFRKFSDMVGQVERLTFARPHKHWKARGLDFSKILSKVKPIFPTDLYC
                     TKSQDHGLDVQLDRELIERCKPALEKGEKVAFDITIRNTNRTVGAMLAGESAMRYGES
                     GLPDGAIHIRFFGVAGQSFGAFVNKGITLEIFGQVNDYVGKGLSGGRLIVRVPEGTSY
                     DPAKNIIAGNTCLYGATGGEAYINGLAGERFGVRNSGAHAVVEGLGDHGCEYMTGGRV
                     VVLGKTGRNFAAGMSGGIAYVFDMDGTFPRMVNPGMVDLEEPDARDEDEILQLVTRHK
                     QYTGSLVAEFILGDWDRLRRKFVKVIPGDYKIALKKIEEEHQLLTEPTETQSAGH"
BASE COUNT         1296 a         1421 c         1227 g         1310 t
ORIGIN      
        1 taagccttgc gaatctcagg accaatcttc ttcacttgct tcttaaacag tggatccgat
       61 tcgctcaaaa acttcagatc ggaccaggcg atcgcagcaa tttctttttc aatctctttc
      121 tgaatggatc ctacaatctg agatccatcc tctgcataca ctttgtatcc attgtattcc
      181 ggaggattgt gactggcggt gatcactact ccagaagcag cctgaagtct gcgaaccgca
      241 taagataaaa tgggcgtagg cgccacatca tcaaaaaggt agaccttgaa tccttgagcg
      301 gccgcaatcc cggccgttac aatggaaaac tcatcgctct ttctacggga atcataggcg
      361 atgaccaggg ctggcttgcg cgatctcttt gcgaatacgc gacagagagc cagagtggcc
      421 cgacccaccg tgtaacgatt catccgaccc gcaccatgcc ccagaacgcc ccgcaggcct
      481 cctgtgccga attcgagctc tccggagtag gcctctacct cctccgaatg cttgcccgcc
      541 atgatgtccg cgtggacgcg gacggcctct tccctgattt ccagcggaaa agagggacct
      601 gcccagtcca ggattctggc ccgcacggct tcaggaatgg catgtttgga gctttctggc
      661 atgtaggaac ttttcctccc ggggaccctg gagtaaattt ttatttgacc gcaggggagt
      721 gtgggtcaat ttttcttttt ctgggccatg attaccgccg agccgcgcaa taatactctg
      781 atcctccatg taaccgaacc tcgtgtagac atggtggtgg cacgcaagtt ccgagaggga
      841 ctcacggcct acctcaagga caagccgcat cgcatcgtgc tcgatctcaa agagacagag
      901 tactttgaca gttccgctct tggtgcactc gttgctttca tgaaagacgt aaaagcatac
      961 ggcggccagc tggttctttg caatctttcc agatccctcc tgactctact caagctttcc
     1021 aagctggatc tccttttcga aatcagcgaa gatctcgaaa aaagcatcgt ttaaagtcca
     1081 gacacccgca atttcacctc tccagaggtc gattttctat aagtggaaga aagcgctact
     1141 tccgtcatca tcgagcagct ccagaaactc tctggatcgc gacgtgagga cttcactgaa
     1201 gatacgatca tcggatctga cataaatcag attcgccata ctttctattt ccttcgctcc
     1261 gagcgcattc cagtcatcat ctcctacatg ggcgaaacct accaggcaag ggtagaacga
     1321 gttgaaaacg atcgaatcat tctgaatgcc ccaggattct ccgagggaac tgcgcgtaga
     1381 tgtagactca agttcgaaat cataaatatt ctataccagt tcgaagtccc cattgtggat
     1441 ctctctcgcg acaagatagt agtgcgcatg ccggctttcg ttcagtctgc gcgacaccgg
     1501 aagtatgttc gtgtttaccc ccaggatttg ttcatgcgat tcaatctggt gtacatgccc
     1561 atgttcggta gacgcggtgt gggtcagatc gtggaaaatc gctatcccaa gattgtgcgc
     1621 gagctcgaaa gagacaggcc ggatcttggt ttgctggtga gaattgtaac ggaggcaatc
     1681 aacgaaatca gtcctgatta tgaattcgtc atgtacaatc cagacgtaaa acgaactttt
     1741 ctcgaacgag tcatgatgag cgaaaaaaag actctgtttg tgcgcgatac atcgagacag
     1801 gatacgtact attcgccact cgggatcttt ggcattacaa actttgaaaa ggaattccga
     1861 agaattgccc gcgaagaatc cattgaagaa gcagaaaatt tctttgagca atatcgccgc
     1921 gcagaactgg tcgatttcat tgcaaattac atatgcacgc ccatcattct acttgatcga
     1981 gtcgtggggc atatcaagat ctattccaca gtactggaca agagaaatct caacccggat
     2041 gaagctcaaa gaatcgatct actggttcaa ctattgaact atgcactcag caaacatgcg
     2101 attgcagaaa catattttac gctgcacaat acgaaggtca tcaacataag catgagtggt
     2161 ctgctctttg agcttcagga cagcaacgta ttcaactatc tgctcgatca tgatcgattg
     2221 aagatctgca tcccgatcaa agcgcacgaa gttgaactgc gcgccgaaat taccaggctt
     2281 ttgcctgcgg agaatggtta caggattgga gtgatgttct tttcaggcgc tccggacgac
     2341 tttcattttc tcgaagggtt cattcacgat cgcatcaaag cgatgcttcg ctaagcgttt
     2401 gcgcacctga tagatggtgc ggaaacaagc cgcaaatgcg gcttttccat caaacagttt
     2461 tcagcttcgc aagcaggttg cggtgaacct gggccgcaca tttacggcct tcagagatag
     2521 cccagacaat caaagactgc ccgcgacgaa cgtctccgca tgcgtaaaca ttgggaagag
     2581 tcgtggcaaa cgttccgtct tcgagtccaa atgtagcttt cacattttta cgttcgtcca
     2641 gagccatgcc catcttagtg agctgcgcga tcaggccgtc ctggactgga tttacgaaac
     2701 ccatggcgag tagaaccaga tctgcttccc atacaaattc acttccagga atatctacga
     2761 acttgccgcc ttcgaacttt gccttgcatc catgaagagt ggccacttcc ccgttcgcgt
     2821 tgcctttgaa ttcctttgtg ctcaccgccc attcgcggat gcatccttct tcgtgactcg
     2881 aagacgtgcg aaggattcgc ggatacgttg gccacggagt agaggaagga cgtgttttag
     2941 gaggttcatc aaagatctcg atttgacgaa cggacttcgc gccatgacga ttcgaggttc
     3001 ccacgcagtc tgaaccagtg tccccgcccc caatcacaat tacattcttg ccttttgcgg
     3061 aaatcgcatc tggtacttga tcgcctgcgt tgatcttgtt ttgcctgcgc aggaaatcca
     3121 ttgcgaaatg aattcctttc agctgacggc caggaagagg aagatcacga ggtacttcgc
     3181 agccaagagc tagaaccact gcgtcaaatg ttgcaatgag ttcttctgct gaaatatcta
     3241 ctccaacgtt tacacccagc ttgaacgtaa cgccttcaat tcgcatctgc tccagacgac
     3301 gatcaatgag ccatttttcc attttgaaat ctggaatgcc gtagcggagg agtccacccg
     3361 cacgatcgtt cttttcaaat acagtaactt tgtgtcctgc ccgcgcaagc tgctgggcac
     3421 atgcaagacc ggacgggccc gatccaacaa cagccaccgt ggttccgagt agtttttgtg
     3481 gagcctgcgg ttctacccat ccttccgaaa agccacgatc aatgatggtt ctctcattgt
     3541 acttgattgt gacagcaggt tcaatgattc caagcacgca ggcgctttcg cacggagccg
     3601 gacacaatct tccggtgaac tcaggaaagt tgttcgtaga atgtaaattt tctaaagcat
     3661 ctctccatct acctttgtag atcaggtcat tgaattcagg tatgagattg tccgttggac
     3721 agccgtattc tccgtggcaa aacggaatgc cacaatccat gcagcgcgcg ccctgagatt
     3781 gcgctgtttc tgcgtccgga gtcttctcaa actctttgta atgcttgatg cgatccgcag
     3841 gcgcttctac tttaagagaa gcacgcgcga attccatgaa tccagtaggc ttacccatag
     3901 cgttcctctc agtgcccggc cgactgcgtt tctgtcggct cagtcaggag ctggtgctcc
     3961 tcttcaattt tcttcaaagc aatcttgtaa tcgccaggaa ttactttaac aaatttacga
     4021 cgaagacggt cccagtcgcc cagaatgaac tcggcgacga gtgaacctgt gtattgcttg
     4081 tggcgcgtaa cgagttgaag aatttcatct tcatcgcgtg catctggttc ttctagatcc
     4141 accatccccg gattgaccat ccgcgggaac gttccgtcca tatcaaaaac gtacgcaatg
     4201 ccgcctgaca taccggccgc gaagttacgg ccagtcttcc cgagcacaac cacgcgcccg
     4261 ccagtcatgt actcgcaacc atgatctccc agtccttcca caaccgcatg cgctccggag
     4321 ttacgaactc cgaatctttc tccggccaga ccattgatat aagcttctcc accggtcgca
     4381 ccatacagac aggtgtttcc agcgatgatg tttttggcag gatcataact tgttccttct
     4441 ggaactcgca caatgagacg accgccagac agacctttgc ccacatagtc gttcacctgg
     4501 ccaaagatct caagagtgat gcctttgttc acgaatgcgc caaagctctg ccctgctacg
     4561 ccaaagaacc gaatgtgaat ggctccgtcc ggcaaaccgg attcgccata tcgcatagca
     4621 gattcaccgg caagcatggc acccacggtg cgattcgtgt tgcgaatcgt tatgtcaaat
     4681 gcgacttttt cccctttctc aagagcgggc ttgcagcgtt cgatgagctc acggtcgagc
     4741 tgcacatcca ggccatgatc ctgagattta gtgcagtaaa gatccgttgg gaaaataggc
     4801 ttcaccttag aaaggatttt ggagaaatcc agaccgcgcg ctttccagtg tttgtgcgga
     4861 cgcgcaaacg ttaggcgctc tacctggcct accatatcgc tgaatttgcg gaaaccaagc
     4921 tgtgccatga tttcgcgcac ttcttgcgca atgaaagtca tcaggttgat tacgttctct
     4981 ggcttaccat gaaacttctt tcgaagctct tcgtcctgag ttgccacgcc caccgggcaa
     5041 gtattgaggt ggcacttgcg catcataatg cagccgacgc ttaccagagc agaagtactg
     5101 aaaccaaatt cctccgcgcc aagcagtgca ccgatcacaa catcgcggcc agtaagaagc
     5161 ttcccatctg ttgcaagcac gacgcggtca cgcagaccat tggcaactaa cgtctggtga
     5221 gtttccgcca gacccagttc ccagggagta cctg
//
LOCUS       DAORFF010000245         5251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8770_length_5251_cov_3.978445, whole genome shotgun sequence.
ACCESSION   DAORFF010000245 DAORFF010000000
VERSION     DAORFF010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8770_length_5251_cov_3.978445"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PKK76_10675"
     CDS             <1..536
                     /locus_tag="PKK76_10675"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HNL68982.1"
                     /translation="HRSPLKHAQPARFEQRVKMLEIVCERLAKNGLSVSVTDMEGKRP
                     PPSYTIESLREIQSQNPSLRISLLVGSDSLADFASWKDPDAIAREFDLMVAYRITDKE
                     AESSIRKVESEFKARITALPGEIPGCASRDIRRWIRSEASGLVKEGGEESLLKDCMAR
                     DVLDYIRAQGLYLNVSN"
     gene            523..1641
                     /locus_tag="PKK76_10680"
     CDS             523..1641
                     /locus_tag="PKK76_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNL68983.1"
                     /translation="MSRISRILLVASAIFVIAASFLFVRRFFSNQLEKRILDRDVVPI
                     RVIVRDQDRIETLAQMILFPEQKWVLFYFVNTDARQPEGSKSIADQTAMFADSFDKYS
                     GLKSIYSVELSRDSFARLIDLGEGLPMYLETAQIFPNSNFQYPAGRMRFSGQQAAEYA
                     LSRRMQQGQEQLSGLERMRRMETILLNVAWNSVQFADKIHSKDLRQFALGLIESDLTK
                     PELASLFNFLSDANISILEVPLEQGADRALYVKDKRARSVYSEFFDNLRSGRMKKDVF
                     SLEVLNGTEVGGLARRVKQFLQDRGILVLNADNYPIKPIPKSVILVRAGKTALGKHVM
                     EMTSLPREGVFFDRSTQDVDCTILLGTDFEIKKLQVFE"
     gene            1644..2093
                     /gene="rsfS"
                     /locus_tag="PKK76_10685"
     CDS             1644..2093
                     /gene="rsfS"
                     /locus_tag="PKK76_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNL68984.1"
                     /translation="MAKKAVKASAPRKSPTKPGPSEKLVKEVTELLQEKKASRIVWMN
                     LTAVNPYFDFFLLASAGSRVQLKSLVKDIEKKFPSLERRNSSISADDMDSGWLVLDFG
                     DVVVHLFLEEQRRYYNLERLWGDAVITEIQDESTRESRLLSEQSAES"
     gene            complement(2026..2409)
                     /locus_tag="PKK76_10690"
     CDS             complement(2026..2409)
                     /locus_tag="PKK76_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68985.1"
                     /translation="MMQKSLRITFILALVCGTLLLLPAFAMLVAAGLALTGKMQTQAN
                     TQIVPALIFSLVLLSLSLVPIAAFFLRKTERGMKLLKIAGWIFLIPAAAFPLALGLPI
                     GSAIMTLLIALTGVWTLVSTRPESQ"
     gene            complement(2406..3143)
                     /locus_tag="PKK76_10695"
     CDS             complement(2406..3143)
                     /locus_tag="PKK76_10695"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNL68986.1"
                     /translation="MKTFVLAFGNFFFRWRDTAFSLVFLTAFLTAGIAVLNPDGFQWD
                     VRTSIIGLIIALAGQFVRGITIGYAYIKRGGLNKKIYAETLQRRGVFAHTRNPMYLGN
                     LLLVTGALVSINNIAFYALALPLFYFIYYAIIVAEEEFLGKKFGEDYVKYTKEVNRLL
                     PGDMGRWSETVKEMEFSFKRLIRKEHGSTFVIFAGTCLYNITKFHLRYGIPLEAMTVL
                     WGIVGALVVLQIIAEVLKRTGQLAEAE"
     gene            complement(3170..3895)
                     /locus_tag="PKK76_10700"
     CDS             complement(3170..3895)
                     /locus_tag="PKK76_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNL68987.1"
                     /translation="MQFWPVASVGDNISAENSSWTFGGDVPKSFDAHVSKSVPLYAEG
                     HEIIAKVSDYFVQDGSTIYDLGCSTGELLRKIGERHAGKDIRLIGVDRVEGMLEVARD
                     KCKEDPRIQFECAELLEFEMQPADFVFAYYTVQFVRPAFRQDVINRIWKGLQWGGAFL
                     MFEKVRGPDARFQDILTGLYNDYKLEQGYSSEEIIGKARSLKGVLEPFSTAGNIDLLH
                     RAGFKDCMTIMRYLNFEGTLAIK"
     gene            3956..5005
                     /locus_tag="PKK76_10705"
     CDS             3956..5005
                     /locus_tag="PKK76_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNL68988.1"
                     /translation="MKNQLEQAVREALGKIQHPVYKESLLDIGAFDRIDEDDESFSVH
                     LKSPDADRKQQIAFEAQIRGFLTKINPGKKVKIRFAVDESLKPQEQGNRIKGVKNVIA
                     IGSGKGGVGKSTAAANLAAALKLKGLKVGLIDSDIYGPSIGKMFGVPSKLPLTGDGSN
                     KILPHEVQGIKLMSFAFMLAADQAVVWRGPMLGKAVEQFLYEVIWGDLDVLLIDLPPG
                     TGDVQLSLAQLIDVDGAVIVTTPQNVALQDAARAASMFQQVKIPILGIIENMSEFQCP
                     NCGHITNIFSKNGGSAMAAKMGVPQLGSIPVEKEIMDSGEAGLPITLKDPSGKVAEAY
                     KKIADRILVEVEKFK"
     gene            5005..>5251
                     /locus_tag="PKK76_10710"
     CDS             5005..>5251
                     /locus_tag="PKK76_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68989.1"
                     /translation="MRQIVGMRFFLLLLLIGFSSLAAETVYLRDGRVLTGRITSQTRT
                     DITIVTPQGPMNIQKDQIRRIQYDTAAEDKAREDARKA"
BASE COUNT         1376 a         1229 c         1350 g         1296 t
ORIGIN      
        1 cgcatcgatc cccgctcaaa catgcgcaac ccgcgcgttt tgaacaacgt gtaaaaatgc
       61 ttgagattgt gtgtgagcgc cttgcgaaga atggtctttc ggtgtccgtt acggatatgg
      121 aagggaagag gccgcctccg agttatacca ttgagagtct tcgcgaaatc caatcgcaga
      181 atcccagcct tcgcatttcg ctcctggttg gatcagacag tctggccgat tttgcatcgt
      241 ggaaggatcc ggacgcaatt gcgcgcgaat ttgatttgat ggtagcatac aggatcacgg
      301 acaaagaggc tgagtcttcg atccggaaag tggaatctga attcaaagcg cgcatcacag
      361 ctctgcccgg agaaattcca gggtgcgcaa gcagggatat tcgccgctgg attcgatcgg
      421 aggcgtctgg tttggtaaag gaaggaggtg aggagagcct attaaaggat tgcatggcca
      481 gagacgttct cgactatatc cgcgcacagg gtctttattt gaatgtctcg aattagcaga
      541 attttactcg ttgcctccgc gatctttgtg atcgctgcat cctttctttt tgttcgcaga
      601 ttcttctcca accagctgga aaagagaatc ctcgatcgcg atgtagtgcc cattcgagtc
      661 atcgtgcggg atcaggacag aatcgaaacc ctggcgcaga tgatcttgtt tccggaacaa
      721 aagtgggtgt tgttctactt tgtaaatacg gatgcccgtc agccagaggg ttctaaatcc
      781 attgcagatc agactgcgat gttcgcagac agctttgata agtactcagg tcttaaatcc
      841 atctactctg tagaactttc gagggattcc tttgcccgtt tgattgatct gggggaaggg
      901 cttcccatgt acctggaaac cgcacagatc tttcccaatt ctaactttca atatccagca
      961 gggcgaatgc gcttttcagg ccagcaggcg gccgagtatg ctctttccag aagaatgcag
     1021 cagggtcaag agcagctcag cgggctggaa agaatgcgaa ggatggaaac gatccttctc
     1081 aatgtggcct ggaactccgt tcaatttgca gacaagatcc attccaaaga tctcagacag
     1141 tttgctcttg gactcatcga atctgatcta acaaagcctg aacttgccag tctctttaat
     1201 tttctatcag acgcaaacat aagcattctg gaagttcctc tggagcaggg tgcagacaga
     1261 gcattgtacg tgaaagataa gcgagcgcga agcgtgtatt ctgagttctt cgacaatttg
     1321 agatctggta gaatgaagaa agatgtcttc tccctggaag tgctcaacgg tacagaggtg
     1381 ggcggacttg cgcgcagagt gaaacaattt ctgcaggatc gtggcattct ggtgctgaat
     1441 gctgacaact atccaatcaa acccatcccc aaaagcgtaa ttcttgtgag agccggcaaa
     1501 acggccctgg gaaaacatgt aatggaaatg acgtcgttgc caagagaagg tgttttcttt
     1561 gatagaagca ctcaggacgt agattgtacg attctactgg ggactgattt cgagattaag
     1621 aagcttcagg tatttgaata agaatggcaa agaaagcagt caaagcaagc gctcccagga
     1681 aatctccaac aaagcctggc ccctcagaaa aacttgtaaa agaagtcacg gagctcctcc
     1741 aggaaaagaa ggcaagtcgg atcgtctgga tgaatctcac cgcggtaaat ccgtacttcg
     1801 atttttttct gctggcgtcc gctggatcga gagtgcagct caagtctctt gtaaaagaca
     1861 tagagaagaa gtttccgtct ctggaacgtc gcaatagttc catcagcgcg gacgatatgg
     1921 attcaggctg gctggttctg gattttgggg atgttgtagt tcatcttttc ctggaagagc
     1981 aacgtagata ttacaacctg gaaagactgt ggggtgatgc ggtcatcact gagattcagg
     2041 acgagtcgac acgagagtcc agactcctgt cagagcaatc agcagagtca taatcgcaga
     2101 accaattggc agtcccagcg ccagcggaaa cgcggccgcg ggaatgagaa atatccatcc
     2161 agcaatctta agaagcttca ttcctctctc tgttttacgc aaaaagaaag cggcaatcgg
     2221 aaccagagag agggacaata gaacaagaga gaatatcagg gccgggacta tctgagtgtt
     2281 tgcctgtgtt tgcatttttc cggtgagtgc gagcccggct gcaacgagca ttgcgaatgc
     2341 gggcaagagc agcaaggttc cgcaaaccag ggcgagaatg aaagtgatac gaagagattt
     2401 ttgcatcatt ccgcttctgc aagttggcca gtgcgtttca ggacttcagc aatgatctgt
     2461 aacacaacca gtgcgcccac aattccccac aaaaccgtca tcgcctcgag cggaatgccg
     2521 tatctcaagt gaaactttgt aatgttgtag agacatgtac ctgcgaaaat aacgaatgtg
     2581 cttccgtgtt ccttgcggat cagtctctta aaagaaaatt ccatttcctt cacagtttca
     2641 gaccacctgc ccatgtctcc tggtaggaga cgattgactt cttttgtgta ctttacgtaa
     2701 tcctctccga atttttttcc caggaattct tcttctgcca caatgattgc gtagtagatg
     2761 aagtagaaca gaggaagtgc aagtgcgtaa aacgcaatgt tgttgatgga aacaagtgcg
     2821 ccggttacaa gaagtagatt accaagatac atcgggtttc gtgtatgcgc gaagacgcct
     2881 cttctctgga gagtttccgc gtagatcttc ttgttcagac cgccgcgctt gatgtacgca
     2941 taaccgatcg ttatgccccg gacaaactgg cctgccaggg caatgatcag tccaatgatg
     3001 gaggtgcgaa cgtcccattg aaagccgtcc ggattcagga ctgcgatgcc tgcagtcaga
     3061 aatgctgtca gaaatacgag agagaacgcg gtgtctctcc agcgaaaaaa gaaattacca
     3121 aatgcaagta cgaaggtttt cattttgatt ccgttttgag tattcttgtc tatttaatgg
     3181 cgagtgtgcc ttcgaaattc aaatagcgca tgattgtcat gcagtctttg aagccggctc
     3241 tgtgaagcaa gtcgatgttc ccggcggttg agaacggttc aagtacacct ttgaggctgc
     3301 gggcttttcc aatgatttcc tctgaggagt aaccctgttc caatttgtag tcattgtaga
     3361 gtccggtgag tatgtcctgg aagcgcgcat ccgggcctct cactttttca aacatcagga
     3421 atgctccgcc ccactgcagt cctttccaga ttctattgat tacgtcctgg cgaaatgccg
     3481 gtcttacgaa ttggactgtg tagtacgcga agacaaagtc agcgggctgc atttcgaatt
     3541 caagaagctc agcacattcg aattgaatgc gcggatcttc tttacatttg tcgcgagcga
     3601 cttcgagcat tccttctacc ctgtccactc cgatcagtct gatatctttt ccagcgtgcc
     3661 gctctccgat tttgcggagc aattctcccg tggaacatcc cagatcataa attgtggagc
     3721 catcttgcac aaagtagtct gaaaccttgg cgatgatttc gtgcccctct gcgtacagag
     3781 gcacagattt gcttacatga gcatcaaacg atttgggaac atctcccccg aacgtccaac
     3841 ttgagttttc agccgagata ttatcgccaa cggaagccac aggccagaat tgcacacatc
     3901 tgctgtacgg aaaagtgttt tcaccggccc ctggtccaca tttcttgcaa ggcatatgaa
     3961 aaatcagctt gaacaggcag tccgggaagc cctcggaaaa atccagcatc cagtttacaa
     4021 agagagcctg ctcgacattg gcgcatttga tcgaattgat gaggacgatg agtcattcag
     4081 cgtccatttg aaatcaccag acgcagacag aaaacagcag atcgcctttg aggcgcagat
     4141 ccgcggcttt ctgacaaaga tcaatcctgg aaagaaagta aagattcgct tcgcagtcga
     4201 tgaatcgctt aaacctcaag aacagggtaa tagaatcaag ggcgtcaaaa acgtaatcgc
     4261 cattggctct ggaaaaggcg gtgtcggaaa atccacagcg gcggcgaacc tcgcggcggc
     4321 attgaagctg aaaggattga aggtgggttt gatcgattcg gacatttatg gtccgtcgat
     4381 aggaaagatg ttcggtgtgc cgtccaagct tcctctcaca ggagacggaa gcaataagat
     4441 ccttcctcac gaagtgcagg gaatcaagct gatgagtttc gcatttatgc tggctgcgga
     4501 ccaggcagtc gtctggcgcg gcccgatgct tggaaaagca gtagagcagt tcctctatga
     4561 ggtgatctgg ggagacctcg atgtgctctt gatcgatctt ccgcctggta ctggagacgt
     4621 tcagctttct ctggcgcagc tcatcgacgt agatggagct gtgattgtga caactccgca
     4681 gaacgtcgca ctacaagatg cggcccgggc tgcgtccatg ttccagcagg tgaaaatccc
     4741 gatccttggt atcattgaaa acatgtcaga gttccagtgc ccgaattgtg ggcatatcac
     4801 gaacattttc tcaaagaatg gcggctctgc gatggcggcg aagatgggag tgccccagct
     4861 cggatccatc cctgtggaaa aggagattat ggattccggg gaggctggtc tcccgattac
     4921 tctcaaagat ccatccggca aagttgcgga agcatataaa aaaattgcag acaggatact
     4981 cgtggaagtt gagaaattca aataatgcgt caaatcgtgg gtatgcgatt cttcctcctg
     5041 ttgctcctga tcggcttctc atctctggct gctgaaaccg tctacttgcg agatggtcgt
     5101 gttctcactg gaagaatcac cagtcagact cgcacggata tcacgattgt gactcctcag
     5161 ggtcccatga atatccagaa ggaccagatt cgtcgcattc agtacgacac agctgcggaa
     5221 gataaagccc gcgaagatgc acgcaaggcg g
//
LOCUS       DAORFF010000246         5241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8796_length_5241_cov_4.124180, whole genome shotgun sequence.
ACCESSION   DAORFF010000246 DAORFF010000000
VERSION     DAORFF010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8796_length_5241_cov_4.124180"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="PKK76_10715"
     CDS             complement(<1..1205)
                     /locus_tag="PKK76_10715"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNL68990.1"
                     /translation="MAKKKTTVESHDFSVLETLRGKTLFVIGGTGFLGRVMLYYFVKY
                     VPDVKLVLLVRPTHGRTGQDRLEKEVLNSPVFTTNPEDKDLRKRALAMIEVVDGDATR
                     PGLGIPSEKSAKIAERIDAVLNTAGNVEFNPPLDLSLSANTLATMQALEFAESTRGKK
                     YVHISTCYVADRTVHRDYAPEIPVASTIVTSRGNEIRIDVQKEIEASRAKIEELKAEF
                     DGEPRMAEFRERAARELKKMGRADGDRMVDKIAKNIRTFELREALTRAGKERAERINR
                     PNVYTYTKTLAELLVLAKTEVESVIVRPSIVEASVRTPFVGWNEGIQGSAPIIFMMHR
                     GHRMIPSLSKNPGEREDAVLDLIPVDFVAAGTILALSALLRGKARQVYQLAAGPIRTP
                     MTITRCLHIS"
     gene            complement(1209..2894)
                     /locus_tag="PKK76_10720"
     CDS             complement(1209..2894)
                     /locus_tag="PKK76_10720"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNL68991.1"
                     /translation="MAEKTPAAELPIEEHVKVVHSRLLREKPAPYVPKGEDDPEVVTI
                     RPGAAKRAMFSGEDIVEIDLPVGSRVIYPKAPIRGLPDPDEAIRHALMHPEKMNPLPH
                     YLKPGMKVTICIDDISLPLPPMERPDMRERILHIVLEYLDNAGVTDVHLIVATAFHRR
                     MTDGEIRRMVGPEIFKRFHPDRLYNHDAENKEGMKWLGHAEKGEAVEINRRAAESDLI
                     IYVNINFVPMNGGHKSVATGLAGYKTLREHHDPEVIANCNSYMDPDGSELARSNKRVG
                     SFIEARIPIFHIESALNNRMFKGPLQFLSKNEDDFNAVEKRAMTALKQSLKRMTPDIK
                     RKIFQRVYAPYEPIAVYAGQVDSVHDRILRASRRQYTVRIRGQADVLIMGIPFVSPYS
                     VNSILNPLLVQVMALGYLYHLYEGGVPLLRDGGTLILFHPCRDEFDPKHHPSYIEFFH
                     RILPEGTDSLTLQKKYEREFATNPDYIKMYREGNAYHGVHPFYMWYWGETGRRKVGQV
                     IAVGAEDKHVPERMGWQNATTFEAALEMAKKSVGPNPNIVMLHQPPVVVARLV"
     gene            complement(2914..3582)
                     /locus_tag="PKK76_10725"
     CDS             complement(2914..3582)
                     /locus_tag="PKK76_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HNL68992.1"
                     /translation="MKEKAAVFYDVDGTLVHTNILHVYVYYAMRLPKISEKARKVLLM
                     LLFTPLYMLIDQMSRTVFNKLFYRNYKGMPEERLRILGKELVREGLMPNVYRDAMERI
                     KKAKKMKLTQVLVTGSLDFAMKQFAKELGIDHVIANQLEMKAGIATGVLVPPILAGRE
                     KLKAMKDFAARENVDLSRSYAFADSKYDLEMLEAVGYPVAVNPDAGLRKIAESRGWPI
                     VSFT"
     gene            complement(3579..3956)
                     /locus_tag="PKK76_10730"
     CDS             complement(3579..3956)
                     /locus_tag="PKK76_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNL68993.1"
                     /translation="MAEEFHYPAPIDVGEAPTPGKSILVELPNPPSGYAKDEAFVFTL
                     EDGQVVGYFNRCSHVQVPLDMGDGDFLDRSGFIMCRVHGARYDLVTGDALLGPAVRGL
                     TRLELALRDGRIIVTGWKKGRQA"
     gene            complement(4097..4942)
                     /locus_tag="PKK76_10735"
     CDS             complement(4097..4942)
                     /locus_tag="PKK76_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68994.1"
                     /translation="MRKLVLLGALLLAGSAEAATYRLFVHGRSGDNHCQPITSTASAT
                     NDHNNYWGGTVTGLANVRYVGFDGTQNGGAYSWNSCGAQKQLNDAVRIFCTGGNTCEI
                     YTHSTGGLVAAAYFGLNNPSGLSISRIQLLASAAGGSELADISTTYLSWLGIKTLGGQ
                     LDQSVSTRGARNGFNHNNAGGRTFYTTSGEGSDYWNLTSPFLPGKDDGVLANHSLCSV
                     NSVTDVGVSCTRGSAAMSESYRCGFLWLSTCHKSYGRWTPYYTVYTGGSNHSHGDAKA
                     DYNRR"
     gene            complement(5020..>5241)
                     /locus_tag="PKK76_10740"
     CDS             complement(5020..>5241)
                     /locus_tag="PKK76_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL68995.1"
                     /translation="RNNSPVTPSMVRKALAENREISGNHTEPLWEYMNMAPNYTTKSY
                     SADDFSKEEWNSEEKNQRIEFLKKQVEQE"
BASE COUNT         1245 a         1364 c         1223 g         1409 t
ORIGIN      
        1 gatatatgaa gacagcgagt gatggtcatg ggcgtgcgaa tgggtcccgc cgcgagttga
       61 tacacctgac gcgccttgcc acgaagaagt gcacttagcg ctagaatggt tcccgctgca
      121 acgaaatcga ccggaatcaa atcgagcaca gcatcttctc tttcacctgg attcttcgat
      181 agagaaggaa tcattctgtg tccgcggtgc atcatgaaaa tgatcggagc agaaccttga
      241 atgccctcat tccagccgac aaacggtgtg cgaacggacg cctctacgat ggacggacga
      301 acaatcactg actctacttc cgtttttgcg agaaccagaa gctctgccag agtctttgtg
      361 tatgtgtaga cgttaggacg attgattctc tctgcgcgct ccttgcctgc tcgcgtgagt
      421 gcttcgcgga gttcaaatgt gcgaatgttt ttggctatct tgtctaccat tcgatcgccg
      481 tctgcgcggc ccatcttctt gagctctctg gcggcccgtt cgcgaaactc agccatcctt
      541 ggttccccat cgaactctgc ttttagttct tctattttcg cgcgagatgc ttcgatctct
      601 ttttgcacat cgattctgat ttcgttgccg cgggaagtta caatggtgga tgcaacagga
      661 atttcaggag cgtagtcgcg atgcaccgtg cggtctgcca cataacaggt agatatgtga
      721 acgtactttt ttcctcgcgt gctttctgca aactctagag cctgcatggt ggccagcgta
      781 ttggcgctca gactcagatc cagtggagga ttaaattcaa cattgcccgc agtattcaac
      841 actgcatcaa ttcgttctgc aatctttgct gatttttcag aaggaattcc aagaccagga
      901 cgagtggcgt ccccatccac aacttcaatc attgccagag cgcgcttgcg caagtctttg
      961 tcttccggat tggttgtaaa aacaggcgaa ttcaagactt ccttctccag cctgtcctgg
     1021 ccagtacgtc cgtgcgttgg tcgcaccaga aggacaagct tcacgtctgg aacgtacttc
     1081 acaaagtagt acaacataac gcgacccagg aagcctgtgc caccaatgac gaataacgtc
     1141 tttcctctca aagtttccag aacggaaaaa tcatgagact caactgtggt tttcttcttt
     1201 gccatacctt agaccagtcg cgcaacaacc actggtggct ggtgcagcat tacaatattt
     1261 ggatttggac caacggactt ttttgccatt tcgagcgccg cttcgaatgt agttgcattc
     1321 tgccatccca ttctttcagg gacgtgcttg tcttctgctc cgactgcaat cacctgccct
     1381 acctttctac gccctgtctc tccccagtac cacatataga acggatgaac tccgtgatac
     1441 gcattgcctt cgcgatacat cttgatgtag tctggattcg tggcgaattc tctttcgtac
     1501 ttcttctgca gtgtgagcga gtctgtacct tcgggcagaa ttctatggaa gaattcaatg
     1561 taactcggat gatgctttgg atcgaattcg tctctgcatg gatgaaagag gatcagagta
     1621 cctccgtcac gcagaagtgg aactcctcct tcataaagat gatagagata ccccagcgcc
     1681 atcacctgaa caaggagtgg attgagaatc gagttcactg agtacggact cacaaatgga
     1741 attcccatga tgagcacatc cgcctgcccg cgaattcgca cagtgtactg cctgcgactc
     1801 gcacgtaaaa tgcggtcgtg cactgaatcc acctgacccg cataaaccgc gatcggctca
     1861 taaggagcat acactctctg aaatatcttt cgcttaatgt ccggagtcat ccttttgaga
     1921 ctctgcttga gagcagtcat ggccctcttt tccactgcgt tgaagtcgtc ttcgttcttc
     1981 gaaagaaatt gaagaggacc cttgaacatt ctgttgttga gagcagattc aatatgaaag
     2041 atcgggattc ttgcctcgat gaacgaccct actcttttgt tggagcgagc cagttcagaa
     2101 ccgtccggat ccatatagga attgcaattc gcgattactt ccggatcatg atgttcgcgc
     2161 agagttttat aaccggcaag acccgtcgca acggacttgt gtcctccgtt cattggcaca
     2221 aaattgatgt tcacgtaaat gatcaggtca ctttccgcag cgcgtcgatt gatttctacc
     2281 gcttctcctt tttcagcgtg gcccagccat ttcattcctt ctttgttttc cgcgtcatga
     2341 ttgtagagac gatccggatg aaagcgtttg aagatctcag ggcccaccat tctacgaatc
     2401 tctccatccg tcatgcgacg atgaaacgca gtggccacga tcaaatgaac atctgttacg
     2461 ccagcgttgt ccagatactc aagaacgata tggagaattc tctctcgcat atccgggcgt
     2521 tccatgggcg gtagggggag agaaatatca tcgatgcaga tggtcacttt catccctggt
     2581 ttcaaataat gaggaagagg attcatcttt tcgggatgca tcaaggcatg gcgaatcgct
     2641 tcgtctggat ctggaagacc gcggatcggg gctttgggat aaatgacgcg tgaacctacg
     2701 ggtagatcga tttctacaat atcctctccg gagaacatcg ccctctttgc cgcacccggc
     2761 cgaatggtga ctacttcagg atcgtcttca cctttgggta cgtaaggtgc cggcttctcg
     2821 cggagtagac gcgaatgcac tacttttaca tgttcttcta taggtagttc agctgcgggt
     2881 gttttttctg ccatgttctt cctgcttaaa aaactaagta aaacttacga ttggccatcc
     2941 gcgggattct gcgatcttgc gcagccccgc gtccgggttt accgcaaccg gatatccgac
     3001 tgcttctagc atttcgagat catatttgga gtccgcaaac gcataggagc gcgacagatc
     3061 cacattttcg cgcgcggcaa aatccttcat tgctttgagt ttttcgcgcc cggcaagaat
     3121 cggaggcacg agcactcctg tggcaattcc tgccttcatt tccagctgat ttgcaatgac
     3181 atggtctatc ccaagctctt ttgcaaattg cttcattgca aaatcaagag agcctgtaac
     3241 cagaacctga gttagtttca tttttttagc tttcttgatt ctttccattg catcgcgata
     3301 cacgtttggc ataagccctt cgcgcacaag ttccttgccc agaatgcgaa gcctttcttc
     3361 cggcattcct ttgtagtttc tatagaagag tttattaaaa actgttctac tcatctgatc
     3421 gatgagcata tagagcggcg taaaaaggag catcagcagt acttttcgcg ccttttcaga
     3481 aatcttgggc aacctcattg catagtatac atacacgtgc aaaatattgg tgtgaacgag
     3541 tgtgccgtcc acatcgtaaa atacagctgc cttttccttc atgcctgtct tcctttcttc
     3601 catccagtta cgataatacg cccgtcacga agtgccagtt ccagtcttgt cagccctctc
     3661 acggcagggc caagcaaagc gtctcctgtg accaggtcat accgggcacc gtggactctg
     3721 cacataatga aaccgcttct atccaaaaaa tctccgtcgc ccatgtccag tggaacctgc
     3781 acatgcgaac accgattgaa atatcctact acctgtccgt cttcgagggt gaagacgaat
     3841 gcttcatcct ttgcataacc agacggagga ttcggaagct ccaccagaat ggatttgcca
     3901 ggcgtaggcg cctcacctac gtcaatcgga gccgggtagt gaaactcttc ggccattcga
     3961 ccagaagccc aaaaaggcct atttacgaaa tgaaaaaagc ccctctagaa aaacagaccg
     4021 ttagagagtg acacaaggta gaaacaaaaa aagccggcac caggccggct ttccagactt
     4081 acaatcaaag atccaatcag cgacgattgt aatccgcttt tgcatctccg tgtgagtgat
     4141 tgctgccacc ggtgtataca gtgtagtatg gtgtccagcg gccgtaagat ttatggcaag
     4201 tgctcagcca caggaatcca caacgatagc tttcgctcat agctgcactt ccccgagtac
     4261 aggaaactcc gacatctgtt actgagttca cgctgcagag gctgtgattg gcaagtacgc
     4321 cgtcatcttt tcctggaagg aaaggactgg tgagattcca gtagtcagaa ccttcgccgg
     4381 aagtggtgta gaatgtacga ccacctgcgt tgttgtggtt aaaaccattt cttgcaccgc
     4441 gtgtgctcac agactgatcg agctgaccgc cgagagtctt gatgcccagc caggaaaggt
     4501 aagtcgtaga aatgtccgct agttcagatc cacctgctgc agacgccagc agttgaattc
     4561 tgctgatgct gagtccagac ggattgttca aaccaaaata agcagcagcg accaggccgc
     4621 ctgtagagtg agtgtagatt tcgcatgtgt ttccgcctgt gcagaaaatg cgaaccgcgt
     4681 cattcaactg cttctgcgct ccgcaggaat tccagctgta tgctccgcca ttttgagtcc
     4741 catcaaaacc aacataacga acgttggcca gaccagtgac tgttccaccc cagtagttgt
     4801 tgtggtcgtt tgtggcgctg gcagtgcttg tgattggctg acagtgattg tccccggatc
     4861 taccgtgaac aaagagtcta taagtggcag cttctgcgct gcctgccagg agcaatgctc
     4921 ccagaagtac gagttttctc atcttagata tccccctatc taacaatgct cagaatgcca
     4981 cggcactctg gccgtgacaa ctactttttc agactcgagt tattcctgct ccacttgctt
     5041 ctttaagaac tcaattctct gattcttctc ttctgagttc cattcttcct tggagaagtc
     5101 atctgcggag tagctcttcg tcgtataatt aggagccatg ttcatgtatt cccagagcgg
     5161 ttctgtatgg tttcctgaaa tctcccgatt ttctgccagg gctttgcgca ccatggaagg
     5221 agtcactgga gaattgttgc g
//
LOCUS       DAORFF010000247         5240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8799_length_5240_cov_4.466924, whole genome shotgun sequence.
ACCESSION   DAORFF010000247 DAORFF010000000
VERSION     DAORFF010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8799_length_5240_cov_4.466924"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="PKK76_10745"
     CDS             <1..1089
                     /locus_tag="PKK76_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HNL68996.1"
                     /translation="RNGGPKKPMNRTDRIRTALPGFEVPAQLLQFIETASPEDLFRVN
                     LVTLSTKINVPLDTLLSAFLKMVEAGLFNLSWEYHCTHCNAIPDFKHSFSELKAESYC
                     PLCDLGFRNTLDKNVEVTFTAHNSFVEIPSEIPERIKAEAIAAVKEKRYQMPEHFLSG
                     LQCLVNETFHQLFGEQVLSSEESLAVETITLLFTDIKGSTQMYSDLGDTKSYEIVREH
                     FKILFQAIVQNGGVVVKTIGDAVMGSFLKPVNAIRAAVQVHDDFNKREWTSIGYLQIK
                     MGIHTGHAIVVNLNDTVDYFGNTVNLAARVQASVKNHDIRFTKRVLEDSEVVSYLKER
                     KAILHKKFEMFKGINDPVEVYRFTPGAP"
     gene            complement(1090..2292)
                     /locus_tag="PKK76_10750"
     CDS             complement(1090..2292)
                     /locus_tag="PKK76_10750"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNL68997.1"
                     /translation="MMEEVIVIGSGHLANRIRQVLEKKSYSVCHLSAEGIESSPDYSV
                     ARLQSIKTQLKHAGAERARAIYVVDSEDRRNIEFALAAMSFERDEPIFLALFNEKIAP
                     HFQINCKNLFIMNPARLAASTFADAVTQVRQAPLPAMAQKPEEGEPDSGIFNWLRSNV
                     LLTVLLSAFLLLYTAGAIFFRYSENLRWIDAFYFITTVITTTGFGDIHLRYSSDEAKL
                     FVICTMLTSVSFFSIIFALVVDKLMERRSQVLLGRKTHRLKGHVILCGLGRLGYQIAL
                     ELRRRGFQIVVIESNEHNRFLNTFRARGIKILYGDATLLRNLEMAGLLHAVALFSVIN
                     DDLTNLEIGLHARSLDPSARLILRIYDRETAEAVRRRLNIEFAYSTSAIAADEMVRAL
                     ESIELARN"
     gene            complement(2289..3128)
                     /gene="panC"
                     /locus_tag="PKK76_10755"
     CDS             complement(2289..3128)
                     /gene="panC"
                     /locus_tag="PKK76_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNL68998.1"
                     /translation="MKIIRSLAELRETLSVRDKVRSLVPTMGYLHEGHASLIRDARRE
                     SEQVCVNIFVNPLQFNNARDFETYPVSEDHDRAICEEAGADIVFVPTASEMLGAGPAL
                     QMAMPALTKNLCGPFRPGHFEGVLLIVAKLFHLFLPHKAYFGKKDYQQFRVIQRMVED
                     LSFPIDIIGCETIREADGLAMSSRNKNLKPEHREQAALMIRGFRIAEKAFNNGNANPA
                     DLAEIAQDVMLTGPDNRVEYTQVVDPETLEVLQEIEGDFVIAAAMHCGEVRLIDNLLI
                     RGG"
     gene            complement(3128..>5240)
                     /locus_tag="PKK76_10760"
     CDS             complement(3128..>5240)
                     /locus_tag="PKK76_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNL68999.1"
                     /translation="FHAPPIVFASLTLAMCLVWIGLALRIKKDYLRALVANLNSRNID
                     LQRNAFSLLSTNRDLETREILLAALSSNDEDMALFALELLDHPDLATALRLSAVLPHT
                     TPRLKTALLGYLGRAGFSETRSAIRTEVSDSDGPVRAAAVRALSLVGSREDVRSMARL
                     IKDTHANVAAEAVIAQLTRPGGQRTVGTKGLDRLLRSREEPDRFQAARILREVPLPAK
                     AKSLVRLMEHSTADVRNEAVRAMGMLKTREALRLLAAYLREDDVYLSAKEGLLQYGES
                     ALSALHAQLAIETEAEAKISIVACIGEISSLRSIPVLAKLIVRQPILVENAAAEALTN
                     IRMKFLVETGQDVSRADEYFTREVKDRIRACYFSIVENIEKDRQNVAILQKASDPTAV
                     LLILDAVQRFARQRLEVALKFLELIGDPRTIRALTADLQFGDARGRAEALEALEGSCT
                     EAGVLVRVLEDDPAQASRGTVDEVLRHILWKNHPPWITSCAVHAAGLLNMKMLEPELF
                     SQLDAPDEMVRINASIALRKLKSKTFRLAVQKERNHKQLERERKKMDTRMERFLFLRS
                     VPLFSEVDGVDLHWINEITHEQKFRARANIFREGDPGESLYIIISGSVRIFKGQKGKE
                     LTIDILQERDCFGEMAILDQEPRSASVVAVRNTRLLVINRSDFQRLLIARPRISFALF
                     KTMSRRVREANARLIQLQKVV"
BASE COUNT         1338 a         1383 c         1250 g         1269 t
ORIGIN      
        1 cggaacgggg gtccaaaaaa gcccatgaat cgcacagatc gcattcgcac ggctctcccg
       61 ggatttgagg tgccggccca attgctccaa ttcatagaaa ctgcgtcccc ggaagatctt
      121 tttcgcgtaa atctggtcac actgagcaca aaaatcaatg ttcccctgga cacgcttctt
      181 tccgcgtttt tgaaaatggt ggaggcagga ttgttcaatc tatcctggga ataccactgc
      241 actcactgca atgcgatccc ggactttaag cacagtttca gcgaactgaa agcagaatcc
      301 tactgcccac tgtgcgacct gggatttcgc aacactctag acaaaaacgt cgaagtcaca
      361 ttcacagctc ataattcgtt cgtcgaaatc ccgtcggaaa taccggaacg catcaaggcg
      421 gaagcgatcg ctgcagtgaa agaaaaacgc tatcagatgc cggagcattt tttgtccggt
      481 cttcaatgcc tggtgaatga aacatttcac cagctcttcg gagaacaggt gctatcctca
      541 gaggaaagcc tggccgtaga aacgatcact cttctcttca cggacataaa gggctccact
      601 caaatgtatt cagatcttgg cgacacgaag tcctacgaaa tcgtgcgaga gcatttcaaa
      661 atccttttcc aggcgattgt tcaaaatggc ggagtcgtag taaaaacaat cggcgacgct
      721 gtgatgggaa gttttctcaa gccagtgaat gcaattcgcg cagccgtcca ggtgcatgac
      781 gatttcaaca agcgagagtg gacctccatc ggctaccttc agatcaaaat gggaattcac
      841 accggtcacg ccattgtagt caacctgaac gacactgtag actactttgg gaacactgtg
      901 aacctggctg cgcgtgtgca ggcatccgta aagaatcacg acatccgttt cacaaaaaga
      961 gtgctcgagg attcagaagt tgtttcgtat ctcaaagaga gaaaggcaat cttgcacaag
     1021 aaatttgaaa tgtttaaagg aatcaatgat ccagtcgaag tctatcgctt cacgccgggc
     1081 gctccttgat caattgcgcg ccaattcaat agattcaaga gcgcgaacca tttcatctgc
     1141 agcaatcgca gacgtactgt acgcaaactc tatgtttaat cgtctgcgaa ctgcttctgc
     1201 agtctctcta tcataaattc gcagaatcaa gcgggcagac gggtcgagtg accgcgcatg
     1261 caggcctatc tccaggttcg tgagatcatc attgatcacg gaaaacagag caacagcatg
     1321 cagcaacccg gccatttcca gatttcgaag caacgtcgca tctccgtaca gtatcttgat
     1381 cccgcgggcg cgaaatgtat tcaagaatcg gttgtgctca ttgctctcta tgacaacgat
     1441 ttgaaaaccc cggcggcgca actccagagc tatctgatat cccagtctac ccagtccgca
     1501 aagaatcaca tggcctttga gacgatgtgt tttcctcccc agcagcacct ggcttctcct
     1561 ttccatgagc ttgtctacta ccagtgcgaa gatgattgaa aagaaagaaa cagaagtcag
     1621 cattgtacag atcacgaaga gttttgcttc atcagacgag tagcgaagat gaatgtcccc
     1681 gaacccggtg gtagtgatca cagtggtgat gaagtaaaac gcatcaatcc agcgaagatt
     1741 ttctgaatag cgaaagaaaa tcgcgccagc tgtatagaga agcagaaacg ccgaaagcaa
     1801 aacggtcagg agaacgttgg aacgaagcca gttgaaaatg ccactgtcag gttcgccctc
     1861 ctccggtttc tgagccatcg ctggcagtgg agcctgtcgc acctgagtca cggcatccgc
     1921 gaaagtggaa gcggcgagtc ttgccggatt catgataaat aaattcttgc aattgatttg
     1981 aaaatgcggc gctatcttct cattgaacaa cgccaggaaa ataggctcat ctctctcgaa
     2041 actcatggcc gcaagggcga attcaatatt ccttctatct tcgctatcca cgacatagat
     2101 agcgcgtgcg cgttcagctc ctgcgtgttt gagctgggtt ttgatgcttt gaagacgggc
     2161 gactgaataa tccggggagc tttctattcc ttccgcgctc aagtgacaca cggagtaaga
     2221 cttcttttcc agcacctgtc tgatgcgatt cgccaggtga ccggatccaa ttacaatgac
     2281 ctcttccatc atccgcctcg aatcagcagg ttgtctatca accgaacttc tccgcagtgc
     2341 atcgcggctg cgatgacaaa atcgccttca atttcctgca atacctcgag ggtctcagga
     2401 tctaccacct gagtatattc cactctattg tctggccccg tcagcattac atcctgggcg
     2461 atctctgcca gatccgccgg gttcgcgtta ccattgttaa acgctttttc cgcaatgcga
     2521 aatccgcgaa tcatcaaggc tgcttgctcg cggtgttctg gtttcaaatt cttgttgcga
     2581 gaagacatgg caagaccgtc tgcttcgcga atcgtctcgc aacctatgat atcaatcggg
     2641 aaagaaaggt cttccaccat tctctggatc acgcgaaact gctggtagtc cttcttcccg
     2701 aaatatgctt tgtgaggcaa aaacagatga aagagcttcg caacaatcaa caatacgcct
     2761 tcaaaatgac ccgggcgaaa cggtccgcac agattctttg ttaacgctgg catggccatt
     2821 tgaagagcag gacccgcacc cagcatttcc gatgcagtcg gaacaaaaac tatatcagcg
     2881 cctgcctctt cgcaaatcgc tcgatcgtga tcttcgctga ccgggtacgt ttcgaaatca
     2941 cgcgcattgt taaactgcag gggattgaca aagatattca cacatacctg ttcactctca
     3001 cgtcgagcat ctcggatcaa agaagcgtgg ccttcgtgta aatatcccat agtcgggacg
     3061 aggctgcgca ctttgtctct tacagaaagt gtttcgcgaa gttctgccag tgaacggatt
     3121 attttcatca aaccaccttt tgcaattgaa tcaatctcgc gttggcttcc cgaactctgc
     3181 gcgacattgt cttaaaaaga gcaaaagaaa ttctggggcg agcgatcagc aggcgttgaa
     3241 aatcagatcg attgatcaca agtagacgag tattcctcac tgcgaccacc gaggcggatc
     3301 tgggttcctg atccaggatc gccatttcac cgaagcaatc cctttcctgt aaaatgtcaa
     3361 tcgtgagctc ttttcctttc tgtcctttga agattctcac gctgccggat atgataatat
     3421 acagcgattc tccgggatct ccttcgcgaa agatattggc tcgggcccta aatttttgct
     3481 cgtgcgtgat ctcattgatc cagtgtaagt ccaccccgtc cacttcagaa aaaagcggaa
     3541 cgctgcgaag gaaaagaaat ctctccatgc gtgtgtccat tttcttgcgc tccctttcta
     3601 attgcttatg attgcgttcc ttctgtactg ccaggcgaaa tgtttttgat ttcaacttac
     3661 gcagtgcaat gcttgcgttg atgcgaacca tctcgtccgg tgcgtcaagc tgactgaaca
     3721 gctcaggctc aagcatcttc atgttgagca atcccgcggc atgaaccgca cacgatgtga
     3781 tccacggcgg atgattcttc catagaatgt gccggagaac ctcatccact gtgcctcgag
     3841 atgcctgggc aggatcgtct tcgagtacgc gaaccagaac gcctgcctct gtacaacttc
     3901 cttcaagagc ttccagcgct tctgcccttc cacgcgcgtc gccaaattgc agatctgcgg
     3961 tgagtgcgcg aattgttcta ggatctccaa tgagttccag aaacttcaac gccacctcaa
     4021 gtctctggcg tgcaaatctc tgcactgcgt ctagaatcaa gagcacggct gtaggatcag
     4081 aagccttctg caaaatggca acgttctgcc tgtctttctc tatattttcc acgatcgaaa
     4141 aatagcacgc ccgaatgcga tccttcacct cacgcgtaaa atactcatcc gcacgagata
     4201 catcctgtcc tgtttcgacg agaaacttca ttcgaatgtt tgtgagagct tccgccgccg
     4261 cgttttccac aagaatcggc tggcgaacaa tcagtttcgc aagcacaggt atgcttcgca
     4321 ggctggagat ttcgccaatg caagcaacga tgcttatttt tgcttctgcc tctgtttcaa
     4381 tggccaattg cgcatggagt gcagacaaag cagattcgcc atactgcaaa agaccttcct
     4441 ttgcggacag gtacacatcg tcttcgcgca agtaagcggc caacaaacga agtgcttccc
     4501 tggtcttgag catccccatc gcacgaactg cttcgtttcg cacgtcagca gtggagtgct
     4561 ccatcaaacg aaccaggctt ttggctttcg cgggcagagg cacttcccgg aggatacggg
     4621 ctgcctgaaa gcgatccggc tcttcgcggg atcgcaataa acgatccagg ccttttgttc
     4681 cgactgtacg ctggcctccg ggccgtgtca gctgagcgat cacggcttct gccgcgacat
     4741 ttgcgtgagt atctttgatc agccgtgcca tgcttcgcac atcttcgcgg ctgcctacaa
     4801 gtgaaagtgc ccgaacggcc gccgcgcgca caggtccatc agaatctgag acttccgttc
     4861 gaatggcgga gcgtgtttca gagaatccgg ctcgtcccag atacccgagc aaggctgttt
     4921 tcaagcgcgg tgtagtgtga ggaagaacgg cgctgaggcg aagagcagtg gccagatccg
     4981 gatgatcgag cagctcaaga gcgaataacg ccatatcttc gtcgttggag gagagtgcgg
     5041 ccagcagaat ttccctggtt tccagatccc gatttgtact caagagagag aaggcgttgc
     5101 gctgaaggtc gatgttacgc gaattcaagt ttgcaacgag tgcccgcaag tagtctttct
     5161 tgatacgaag tgcaaggcca atccacacaa ggcacatcgc gagagtgagc gaagcaaata
     5221 cgatgggtgg agcatgaaat
//
LOCUS       DAORFF010000248         5192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8922_length_5192_cov_3.194666, whole genome shotgun sequence.
ACCESSION   DAORFF010000248 DAORFF010000000
VERSION     DAORFF010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8922_length_5192_cov_3.194666"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PKK76_10765"
     CDS             complement(<1..589)
                     /locus_tag="PKK76_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNL69000.1"
                     /translation="MDFFKSKKHMVLPSASLLPSGDPTLLFNTAGMVPFKAYFAGTEK
                     PPSPRVATIQKCLRTTDLESVGKTERHCTFFEMLGNFSFGDYFKRESIQWAYEFSLKH
                     LNLDPEKIYITVYQDDDEAEEIWHKEVGVPMNRIRRLGKDANWWGPAGDSGACGPCSE
                     LYLDRGEERCNRKPDASCGPGCSCDRFMEYWNLVFN"
     gene            824..1165
                     /locus_tag="PKK76_10770"
     CDS             824..1165
                     /locus_tag="PKK76_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69001.1"
                     /translation="MLAASCAWMGEDGLTAIRGQKVITRKETQGRLREAIIINMAKCP
                     ENVNAGLYGEDYIVPGEVTHTHYYEESVNNCVVAILAVSCGLNPSQNIVILANFYRTV
                     IRLCNLKPATG"
     gene            1270..2028
                     /locus_tag="PKK76_10775"
     CDS             1270..2028
                     /locus_tag="PKK76_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69002.1"
                     /translation="MRRAFISAFVAALLGVAFPRFAETTQTAPQPAKESEDKARRCLN
                     PATRTAQVPSDGRPIMSENAIGNNPQEKQYREEIERLLTIEIQNVKIAVTDVELKHKR
                     LMQLYPRLQYYQEIVLEDVPGAWREGDYVNSKKVLSLTYDLPADGSSKLTCLVMDSMT
                     RNVYHPDQWTRKLMRLYYPNVQMVELETHRHNFSMPSPGAPAITLEATSPEIQLRALR
                     LIYLNLRTALYSMDMLIASYYDKRNRRNEWQINL"
     gene            2123..3354
                     /locus_tag="PKK76_10780"
                     /pseudo
     CDS             2123..3354
                     /locus_tag="PKK76_10780"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            complement(3423..>5192)
                     /locus_tag="PKK76_10785"
     CDS             complement(3423..>5192)
                     /locus_tag="PKK76_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69003.1"
                     /translation="PRYNLQKAKLLLRLGNYSEILSIPVPENPGFTRSQILRLRSGAS
                     LRMKEKVTAAIQTILAYEASPYSGAADDFRYLLLEEKDYRQIQSSNLSKKQVGIFYFL
                     SGIHMSSGGSFSLAVEDLENLWNAEPGTPYADPLLLKTIALMEEESLPVILYHQEPPK
                     QNSGIDSIRIRLLSWIAGKFPQLYAQTNANGLPDSTTLAATRLRKFLEKNKSNSRNPA
                     LWNLAAAYAAYTAGDSDAASAALSAKEKKPAYEVRRQLILLLARIQKERVISEETENA
                     FVNLLPLLSGSSSGMARYSPFDEKGPPAATMMLYELLSDKYKKGGAGHKGIIASYLAG
                     AFEWYDTARLDASSEPDLVRLRDYLAQPPVTSMDRLIKSKFHLSRAVVMDRLAAKYAL
                     QGKYKEAEQELRAAGPPKKITLEYTGDLGPFDATFEDASKNAKNWNRLTFFQEMGKLS
                     AAIDAKPADAEAALLHYRRGVGALSASHFGKWWILMKDYRSVGDVYMGHDWELMLRQA
                     KNDFEAAARLTKDPELGAKSTFLSALASQGLNQPREWTEEKQKLSAAEADSYRKLGTI
                     YKDTRFFNTIGSSCSVLNDFLGH"
BASE COUNT         1116 a         1279 c         1356 g         1441 t
ORIGIN      
        1 gattgaacac aaggttccag tattccatga accgatcgca cgaacaacca gggccgcagg
       61 acgcgtctgg tttgcgattg cagcgttctt ctcctctgtc gaggtagagt tccgagcatg
      121 gtccgcaggc tcctgaatct ccagcaggcc cccaccagtt tgcgtccttt ccaagtctgc
      181 gaattctgtt cataggaacg ccgacttctt tgtgccagat ttcttctgct tcgtcgtcgt
      241 cttgatagac tgtaatgtag atcttttctg gatcgaggtt taaatgcttc agtgaaaact
      301 cataggccca ctgaatggat tcgcgtttga aataatctcc gaagctgaaa ttgccgagca
      361 tttcaaaaaa agtacagtgt ctctctgtct ttccaacgga ttcaagatct gttgtgcgaa
      421 ggcatttttg aatcgtggcc actcgagggg agggcggctt ttctgttcct gcgaagtaag
      481 ctttgaaggg caccattccg gcagtgttga acaggagtgt tggatctccg gaaggcaaga
      541 ggctcgcgct gggcagaacc atgtgcttct tgcttttgaa aaaatccatc cacagcttgc
      601 gaatgcgcgc cgcagaccac gcctctttcg gtagatcggc tggaactttc tttttggccc
      661 cgggggcgga tgcggtgggt ttcttctgtt ttggtgacat gcgctttttt aagaaacttt
      721 tttggaaggc ctgccataaa gcagaatttg taaatttgtg gcaggccttg cgtgcttgcc
      781 gagtctggaa gaaatgcggc ttctggctcc ctttttcgcg ttcgtgttgg ctgcatcctg
      841 tgcatggatg ggtgaagacg gtctgactgc gattcgcggt cagaaggtga ttaccagaaa
      901 ggaaacccag ggaaggttgc gcgaagcaat catcatcaac atggccaagt gcccggaaaa
      961 cgtgaatgct ggtctttatg gggaggatta catcgttccc ggggaagtga cccatacgca
     1021 ctactatgaa gaatcagtga acaactgtgt agtggccatc ctggcagtct cctgcggcct
     1081 caatcccagt cagaacattg tgattctggc taatttctat cgcactgtca tccgtctctg
     1141 caatctcaag cctgcgacgg gctagcttcg acgtttccaa aaatttcttt aagtccttgt
     1201 gtttttttgt tcggccgctt tttattgtgg ggtagtcggg ccgacacata catacgggag
     1261 atatcgacca tgagacgtgc ctttatatct gcgtttgttg ccgcccttct gggtgtggca
     1321 tttcctcgct tcgcggaaac aacgcaaact gcgccgcagc cggcgaaaga gtcggaggat
     1381 aaggccagac gttgcttgaa tcctgcaacc agaaccgcac aggttccgtc ggatggccgt
     1441 ccgattatgt cagaaaacgc aatcggcaat aaccctcagg aaaagcaata ccgcgaagag
     1501 attgaacgcc ttcttaccat tgaaattcag aacgtgaaga ttgcagtgac agacgttgaa
     1561 ctcaagcaca agcgtttgat gcagctctat ccgcgtcttc aatactacca ggaaatcgtt
     1621 ctggaagatg tccccggcgc gtggagagaa ggggattatg tgaactccaa gaaagttctc
     1681 agtcttacgt atgatctacc tgcagatggc tcgtccaaat tgacctgtct tgtgatggac
     1741 tcaatgacgc gtaacgtcta tcatccggat cagtggacac gcaagttgat gagattgtat
     1801 tatccaaacg tgcagatggt ggaactcgaa acgcatcgtc acaacttctc catgccatct
     1861 cctggcgctc ctgccatcac actggaagct acttctcctg aaatccagct gcgtgccctt
     1921 cgattgatct atctaaatct tcgcacggcg ctttacagca tggacatgtt gattgcatcg
     1981 tactacgaca agagaaaccg ccgcaacgag tggcagatca atctctgacg tgtcgtcagg
     2041 gtttaaagac acagaagatt atatactgag aggcggcctc tggcgcctca tggtagatct
     2101 ggcatggccg gcaatcgtcg gcatgtcttt ctatgctctc aacactttcg ttgatgtaat
     2161 tttcattggg cagcttgtgg gagagcaggc ggttgccggg gtgtctctcg cttttcctct
     2221 tgcaagtatt cccatgggtg tggccgcgat gcttggctct ggcggcggtt cgctcctatc
     2281 cattgcaatc ggtgccggcg attccgcgct tcacaaaaga ctttcagggc tcacaggctc
     2341 tcttgcaatc atcgctactc tcccgatggc tgcagccggc gcagtcttcc cgaattattt
     2401 ttgcattgga tgggcggatc cggcgaaccg ttgcgtctgg gtgcccagta tttttcggtt
     2461 ctttccttgt tctttccagt cacagtttac gcaatctcag cgaacatgct ggtgcgcgct
     2521 gaagggagaa tgcgtaccgc gatgaagatt cttgcggtca gcacagttgt aaatgttgcg
     2581 ctcaatcctc tttttatccg cacattcggt ctgggtattc gcggctcagc acttgcgact
     2641 aacgtagcaa tggttgctct tgcagctgca ggcgccgtct attttaggtc cggtaagtcg
     2701 agtgtgagag aacccgtgtc tctttttgca tacgatccta aaatttttaa acgaattatc
     2761 tcgattggcc tttccagttt tattttacaa ggaatgggtg ttttgcagca gatctttgtt
     2821 ttccgtttga tcgcggcgca cggtactgag aaggatgttg ccttgatggg gacaggactt
     2881 cgcatcctgt ttctgtgtgt ttatccggtt atggggcttg ttcgcgctct ccagcctgtc
     2941 attggcatta actttggagc tcgccagtac gcccgagtga tagaagccct gcgtgtcttc
     3001 agcatcggtg gcactgcttt catggcattg atctggcttc ctttgacaat gttctcgcgc
     3061 acggtccttg gatggatgct gccggacacg gccatcacgg acactgacgt tctaaatttt
     3121 cgcatactca ttgtaactct gcctctgatg gcgggagttt tcaacggact cactttcttc
     3181 caggcaatcg gacacggcaa gaccgcaacc atcctcagtg tccaggcaga tattggtctt
     3241 tgtacctgtg gtgctgatcc tgcccgttta ttttggggtg agcgggatct attacagcct
     3301 caccggcgtg gacgttacgt tgattgccgt tcttgcaatg ctcgttctgc gtgaattcaa
     3361 attgctttcc gcaaaaccag acggtgccga acgttgaact ttctgccgtt aggcgccctt
     3421 gttcaatggc ctaagaaatc atttagcact gagcaggaag agccaatggt gttgaaaaat
     3481 cgcgtgtctt tgtaaattgt tcccagcttg cgataactgt ctgcttctgc cgcggaaagt
     3541 ttttgttttt cctccgtcca ttctcgtggt tgattcaagc cctgggacgc aagtgcggat
     3601 aggaacgtgc tcttcgcacc cagttctgga tccttcgtga ggcgagccgc cgcttcaaaa
     3661 tcattctttg cctggcgaag catgagctcc cagtcatgtc ccatatagac gtcacccacg
     3721 gacctgtaat ccttcatgag aatccaccac ttcccaaaat ggcttgcgct cagagctccc
     3781 actccgcggc gatagtggag aagagccgcc tccgcatcag ccggtttcgc atcaatggct
     3841 gcagacaatt tgcccatttc ctggaagaag gtgaggcgat tccagttctt tgcattcttg
     3901 cttgcgtctt caaaggtcgc gtcaaacggg cccagatcac ctgtgtattc cagagtgatt
     3961 tttttgggag gaccggctgc tctaagttct tgctccgctt ccttgtattt tccctgcaga
     4021 gcatacttag ccgcgaggcg atccatgacg acggcacgcg acaggtggaa tttggatttg
     4081 atcaaacgat ccatgcttgt aacgggcggc tgtgccagat agtcgcggag ccgcacgaga
     4141 tccggttctg aagatgcatc gaggcgagcg gtgtcgtacc attcgaatgc accggccaga
     4201 taggatgcaa tgattccttt gtgtccggca ccccccttct tgtatttgtc agagagcagt
     4261 tcataaagca tcatcgtcgc tgcaggcgga cctttttcat caaatggcga ataacgtgcc
     4321 atcccggagg atgaaccaga aagcagcgga agcaggttta cgaatgcatt ttctgtttct
     4381 tcgctgatca ctctctcttt ctgaatcctg gccagaagca gaatgagttg tcttcgcact
     4441 tcgtaggctg gcttcttttc ttttgcagag agggctgcgc ttgcggcgtc gctgtctcct
     4501 gccgtgtagg cggcataggc agctgccaga ttccacagag caggattcct ggaattggat
     4561 ttatttttct cgaggaattt gcgaagtctg gtggcggcga gtgtggtgga gtccggaagt
     4621 ccgttcgcat tagtttgcgc ataaagctga ggaaatttac cagcaatcca ggagagcagt
     4681 cgaatgcgaa tcgaatcgat gccgctattc tgctttggtg gctcctggtg atacaggatc
     4741 accggcaaag attcttcttc catgagagcg atcgttttga gaaggagtgg atctgcatac
     4801 ggtgtgcccg gctctgcatt ccaaagattc tccaggtctt ctactgccag agaaaaggag
     4861 ccgcctgagg acatgtggat tcctgaaaga aagtaaaata tcccaacttg ttttttagac
     4921 agattggacg attggatctg tctgtagtct ttttcttcca gaagcaaata tctgaaatcg
     4981 tctgcagcgc ctgaataggg agacgcttcg tatgccagaa tggtttgaat ggccgcagtc
     5041 actttttctt tcatgcgaag gcttgcaccc gagcgcagtc ttagaatctg agatctggtg
     5101 aaccctggat tttcaggcac agggatggag agaatttcgc tgtagtttcc cagtcttaga
     5161 agtagttttg ctttctgcag attgtatctg gg
//
LOCUS       DAORFF010000249         5184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8945_length_5184_cov_3.286021, whole genome shotgun sequence.
ACCESSION   DAORFF010000249 DAORFF010000000
VERSION     DAORFF010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8945_length_5184_cov_3.286021"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PKK76_10790"
     CDS             <1..194
                     /locus_tag="PKK76_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69004.1"
                     /translation="AIDSLSRSGKPAPAIFPAGPAPRVSAKARPFFYTKLIFTKRTGL
                     RLHAPSISDLQINLHRGVV"
     gene            191..529
                     /locus_tag="PKK76_10795"
     CDS             191..529
                     /locus_tag="PKK76_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69005.1"
                     /translation="MKSGSGAFNIFGLIFCVFMGYTGVSLGFGAAYPPLNLVAKPFVC
                     SNGEMQYQQKSINPLPGRTYTMARWQCTDSSGNSAPVSNAHIAVYSGLIYGIGMFVLL
                     KLLMIFRGRS"
     gene            complement(569..1237)
                     /locus_tag="PKK76_10800"
     CDS             complement(569..1237)
                     /locus_tag="PKK76_10800"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNL69006.1"
                     /translation="MHFVGINLVIALSLISCSMELSTGSQELLQKSSVVLLARLSSLT
                     STPVIICKNQGYCIVFRTTSTFAGTAVGGVSGADTKCNADANKPANTGTYKALITDGA
                     ARVACTSANCATSGIAEHSDWVMYPATEYRQVNQTTVIFTTNANGIFILPGALAGWDG
                     TASSFNSGFNSNWTASGGNHCTAWTIGGGSGMQGIGNSTSATMLQNASIGCGSVVSLL
                     SVQQ"
     gene            complement(1365..3080)
                     /locus_tag="PKK76_10805"
     CDS             complement(1365..3080)
                     /locus_tag="PKK76_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNL69007.1"
                     /translation="MRQDPENNDGVIARSFARHRLLFLAVLTGLTAGLAAVSLKILVH
                     LVSGISTNRLPGSVFSFLPAAGLLLTIVFTRFVLRAQPGTTADILVTIVQRSSVVERF
                     KMISHLVGSALTVGLLGSAGLESPIAITGAAIGSNLGRNVNYKERTLLLSCGAAAGIA
                     AVFNAPVAGMLFAIEVLLLDVSLSSIIPLVAAAVTGSLCSIVLQQNEILFEFPRIQHF
                     DYRNIPFYFILALFCSMISLLYSGIMRLAQRFLSPFPWLLKWGVAGLILWAVGSIAPV
                     VLGEGSRAIAGLTQGLPREFFHSIPLPVPFAVALCIFVVLKIIATAATLKSGGIGGNF
                     APSLFVGACAGFALANAFNAAHPGLLPEGNFTIVGMAGTLSGVMYAPLTAVFLIAEAT
                     GGYTLMVPLLLVSALTHVIVIQLRSHSMELSELEEQGVNPTRRRDAQILGQIRLGDLL
                     EKDFVTVFEDAKLRDLIGAIAISRRNLFPVVTSSGDLVGIVTLNDIRDQIFKSELYDS
                     QFVRDLMVPIRQSIDINDSMQMAMDKFDHSGAWNLPVVESKRYAGFVSRSRLFAAYRE
                     GARSV"
     gene            complement(3189..4001)
                     /gene="otsB"
                     /locus_tag="PKK76_10810"
     CDS             complement(3189..4001)
                     /gene="otsB"
                     /locus_tag="PKK76_10810"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HNL69008.1"
                     /translation="MGGQNDDGYCRSAPAYTFVVAVWRSGLKDILSRKHVNELSLLIA
                     RKPLIAFDFDGTLAPIVDDRDSACMRASTRRLLRRLSRCLPCVVISGRMRSDVCARLE
                     GIPLVAIVGNHGSEWEGDDHDHALLERVRNWKELLKGELPRDGVSIEDKSVSLSVHTR
                     HNVSSPDIRRILNRLTDAKVIGGKRVYNIVPAEAGGKGEALLRMMQECKCDCALYIGD
                     DDTDEDVFALFDSEIMTIRVGQSRNSSAAYYIASQAQIDTLLEKMLSWPPCS"
     gene            complement(3920..>5184)
                     /locus_tag="PKK76_10815"
     CDS             complement(3920..>5184)
                     /locus_tag="PKK76_10815"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNL69009.1"
                     /translation="QVPPGENLYSLRRVWLSQEEERGYYYGFANEGLWPLCHLAHARP
                     MFRATDFRHYSRINARFAAAAAQEGKVRDPIVWIQDYHFALAPQLVRKKLPAATILTF
                     WHIPWPNAERIGICPWHKELIAGLLGSDILGFHTQLHCNNFLSAIDRFLETRIDRERQ
                     SVFYQGHETIVRPYPISVEWPNSWAVAAPDVSECRHALITECNLKQDAVIGLGVDRLD
                     YTKGIAERFLAVERALERFPELRGRFSFVQLAAPSRTEIERYRELSSQIEQIVERINA
                     RFGRGTYRPIVYHRAHHEPPKVFQFYRACDFCYVSSLHDGMNLVAKEFVAARDDERGV
                     LILSNFAGASRELSSALIVNPYDLEEASSAFATAVTMSSEEQQERMRAMRQTVAKFNV
                     YRWAGRMMMDTAGLRQHTRLLSRYGDRD"
BASE COUNT         1323 a         1411 c         1294 g         1156 t
ORIGIN      
        1 cggcgattga ttctttgagc aggagcggca aacctgctcc tgctattttc ccggccgggc
       61 ctgcccccag ggtcagtgcg aaagcccggc ctttcttcta tacaaaacta attttcacaa
      121 aaagaactgg actgaggctg cacgctccat caatctctga tctgcaaatc aatttgcaca
      181 gaggggtcgt atgaaatcag gttcaggcgc cttcaacatc tttgggctta ttttctgcgt
      241 gtttatggga tatacgggtg tctcccttgg ttttggggct gcctaccccc cgctcaatct
      301 agtagcaaag ccatttgtat gctcgaatgg agaaatgcaa taccagcaga agtccatcaa
      361 ccctctgccc ggaagaactt acactatggc aagatggcaa tgcacggatt cgagtggcaa
      421 ttcggctcct gtttccaatg cccacatcgc cgtctactct ggactcatct acggaatagg
      481 catgtttgtt cttctcaaat tgcttatgat ctttcgcgga agaagctgaa catcccccgg
      541 tccgctcaaa gatcgcaagc gtggcgcatc attgctgcac actgagtaga ctcacaaccg
      601 aaccgcatcc aatggatgca ttctgcaaca tggtcgcgct tgtagagttc cctataccct
      661 gcattccgga tcccccgcct atagtccacg ctgtacagtg gtttccaccg ctcgcggtcc
      721 agttagagtt gaatccgcta ttgaatgagc tcgctgtgcc atcccagccc gcaagggcac
      781 ccggaagaat aaatattcca tttgcgttcg tcgtaaaaat gaccgtggtc tgattcacct
      841 gacgatattc cgtagctgga tacatcaccc agtctgaatg ttctgctatt cctgacgtcg
      901 cgcaatttgc ggaagtgcag gcgacgcgtg cggcgccatc cgtgatgagt gccttgtagg
      961 taccggtgtt tgcaggctta ttcgcatccg cgttgcactt tgtatcagcg ccgctgactc
     1021 cacctactgc agtgcctgcg aaagtggacg ttgtgcgaaa tacaatgcaa taaccttgat
     1081 ttttacagat gatgactggt gtagaggtaa gactgctcaa tctggcaagc aacacgacag
     1141 aacttttttg caaaagctcc tgacttcctg tcgaaagctc catggagcat gaaatgagag
     1201 aaagggcgat caccaggttg ataccgacga aatgcatcag ccgccgcatt cttcaacttt
     1261 cgcacagggc gaaagaatcg tcgaatctgt ttttgcgcgc gcgatgcttt tttgtcgcgc
     1321 gcagaaatgc ccgcggcaga acagacaaaa gtcgggcatc atttctatac acttcgcgcg
     1381 ccctctctat atgctgcgaa caaccgcgat cgggacacaa agcccgcata gcgcttgctt
     1441 tcgactacgg gaagattcca ggcaccactg tgatcaaact tgtccatcgc catctgcatg
     1501 ctatcgttga tatcgatgct ctggcggatg ggaaccatca agtctctgac aaactgtgaa
     1561 tcgtacagtt cacttttgaa aatctggtct cgaatatcgt tcaaagtcac aatgccgaca
     1621 agatccccac tcgaagttac gaccggaaat agattgcggc gcgagatcgc aattgcaccg
     1681 ataagatctc tgagctttgc atcttcgaag acggtaacga agtctttctc caacaggtcc
     1741 ccaagtctaa tttggcctag aatttgtgca tcgcgacgcc gggtcggatt cactccctgt
     1801 tcctcaagtt cgctcaattc catcgaatgc gacctcaatt gaataacaat cacgtgcgtt
     1861 aacgctgaaa cgaggagcaa cggaaccatg agagtatacc cacccgttgc ttcggcgatt
     1921 aggaaaaccg cggtcagcgg ggcatacata acgccgctta acgttccggc catgccgacg
     1981 atcgtaaaat ttccttctgg caggagtcct ggatgggccg cgttgaacgc gttcgcaagt
     2041 gcaaaaccag cgcaggcacc aacaaaaagc gacggagcga aattgccgcc gatgcctccg
     2101 ctcttcaacg tcgcggccgt cgcgatgatc ttcagaacaa cgaaaatgca gagagctacg
     2161 gcgaaaggaa ccggaagcgg aatagagtga aagaattccc gtggtaatcc ctgagtcaga
     2221 ccggcgatcg cgcgagaacc ttctcctagt acgaccggcg caatgcttcc gaccgcccaa
     2281 agaatcaggc cagccacccc ccatttaagc aaccacggaa atggcgaaag aaacctttgc
     2341 gcgaggcgca tgataccgga ataaaggagt gagatcattg agcaaaacaa agcaagtatg
     2401 aaatagaagg gaatattacg atagtcaaag tgttgaatcc ggggaaactc aaagaggatt
     2461 tcattctgct gcagcacaat cgaacacaaa gagccagtca cggcagccgc caccagagga
     2521 atgattgacg agagggaaac atccagcaac agaacttcaa tcgcaaagag cattcccgca
     2581 acgggggcat taaaaacggc cgcgatgccc gcggccgctc cgcaagaaag gaggagtgtt
     2641 ctctctttgt agtttacgtt acgcccaaga ttcgaaccaa ttgcggcacc agtaatcgcg
     2701 attggagact cgagtcccgc cgatccaaga agcccgaccg tgagggcgct ccccacgaga
     2761 tgcgagatca tcttaaaacg ctctacgacg ctcgagcgct gcacgattgt aacgaggata
     2821 tccgcagtgg ttcccggttg cgctcgtaaa acaaagcgcg taaagactat agtcagaagg
     2881 agaccggctg caggaagaaa agagaagacg ctgccgggca gacgattcgt actgatccca
     2941 ctaaccaaat gtacgagtat cttgaggcta actgccgcca gacccgcagt gagcccggtc
     3001 aaaacagcga gaaatagaag acgatgacgc gcgaaagatc gggcaatcac gccatcgttg
     3061 ttttctggat cttgacgcaa gtctccgcaa taacgaatac tccatcgctg tcgatagatt
     3121 cttgcaattt tcctccggag tcagtgaccg tggcattgga aagcttctcc gaatcgcaac
     3181 ccattgcact atgagcaggg gggccaggaa agcatctttt cgagcagcgt gtcgatttgc
     3241 gcctgactgg caatataata ggcagcactc gaatttctgc tctggcctac gcgaatggtc
     3301 atgatttcgg aatcgaaaag tgcgaatacg tcttcatccg tgtcgtcgtc tccaatgtag
     3361 agggcgcaat cgcacttgca ttcttgcatc attctcaaca gggcctcgcc tttgccgcca
     3421 gcttccgccg gaacgatatt gtacacacgc ttacctccga tcaccttcgc atccgttagt
     3481 cgattcaaaa ttctacgaat gtctggagat gagacattgt ggcgcgtgtg gacggagagt
     3541 gaaacactct tatcctcgat agaaacaccg tcgcgcggca gctcgccttt gagcaattcc
     3601 ttccaattcc gcaccctctc aagcagagca tggtcatgat cgtccccttc ccattcagac
     3661 ccgtgatttc ccactatggc gaccagggga atgccctcca gccgtgcgca gacgtctgag
     3721 cgcatgcggc cagaaattac tacgcaggga agacatctgc tgagccttct cagtagtcgg
     3781 cgcgtggagg cccgcatgca cgctgaatca cgatcgtcca cgatgggagc gagcgtaccg
     3841 tcaaaatcaa aagcgatcaa aggtttcctg gcaatcaaga gtgacagctc attgacgtgc
     3901 tttcgggaga ggatgtcctt caatcccgat ctccataccg cgacaacaaa cgtgtatgct
     3961 ggcgcagacc ggcagtatcc atcatcattc tgcccgccca tctgtacaca ttgaatttgg
     4021 caacagtctg ccgcatcgca cgcatccttt cctgctgctc ttcggaagac atggtaaccg
     4081 ctgttgcaaa agcggagctc gcttcttcga gatcgtatgg atttacgatc aatgcggaac
     4141 tgagttcgcg tgaagctccc gcgaagttgc tgagaatgag aacgccgcgc tcgtcatcgc
     4201 gcgctgccac aaattccttt gcaaccaggt tcattccatc gtgcagactg ctcacataac
     4261 aaaaatcaca ggcgcggtaa aactgaaata ctttcggagg ttcgtgatgc gcacggtgat
     4321 acacgatggg ccggtaggtg ccgcggccaa atcgagcatt gattctttca acgatctgct
     4381 caatttgact ggaaagctca cgatacctct caatctccgt ccgactgggt gcagcaagct
     4441 gtacgaaact gaaacggccg cgaagctcag gaaatctttc cagagctctt tccactgcga
     4501 ggaatctttc cgcaattccc ttcgtgtaat ccagcctgtc cacgcccaga ccaatgacag
     4561 catcttgctt cagattgcat tccgttatca gagcatgcct gcactccgaa acatcagggg
     4621 ccgccaccgc ccaggaattc ggccattcca ccgaaattgg ataagggcga acaatcgttt
     4681 cgtgaccctg atagaagact gactgtcttt cgcgatcaat tcgagtctcc aggaatcgat
     4741 caatggcaga aaggaagttg ttgcaatgaa gctgcgtgtg gaaacccaga atgtcactgc
     4801 ccagaagtcc agcaatcagt tccttatgcc agggacaaat gccgatccgt tccgcatttg
     4861 gccacggtat gtgccagaaa gtcaaaatcg ttgctgccgg aagtttcttg cggacaagtt
     4921 gcggcgcgag ggcgaaatga taatcctgga tccatacgat tggatcgcgc acctttcctt
     4981 cttgcgcggc ggccgcggca aatcgggcgt tgatgcgcga ataatggcga aagtcggttg
     5041 cgcgaaacat tggccgtgca tgagccaggt gacagagggg ccagagacct tcatttgcaa
     5101 aaccatagta gtagccgcgc tcctcttctt gcgagagcca aactcttcgg agagaataca
     5161 ggttttctcc aggaggaacc tgga
//
LOCUS       DAORFF010000250         5180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_8956_length_5180_cov_3.855805, whole genome shotgun sequence.
ACCESSION   DAORFF010000250 DAORFF010000000
VERSION     DAORFF010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8956_length_5180_cov_3.855805"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="PKK76_10820"
     CDS             complement(<1..1430)
                     /locus_tag="PKK76_10820"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL69010.1"
                     /translation="MRKVLPALLLLFACRPVTAPPSVQAGLIDLRGVDFANTDPIALD
                     GDWEFFPMEFISPGKAVSHSFIAVPGSWNKAPYNGDSLGAHAFGSFRMRILVQTDRPL
                     AFRVGEVGTAYRLICEGQLLGGRGFPSDNPEISVPLTQPATYIFTPHSNQVEVILHVS
                     NFHYRKGGIWHSISFSDANRITDLIVLKQWTSIVVFAMLAILGMYHVAFWFVGRRSFF
                     FFGLVCIDIALRETVTGSKIALLVIPALPWELSIKVEYWTLTWSSGFFALYMNSLFPA
                     FFHRSWTRGMVGLSVLVSIMVLFTPASFYTQTVYLQNGYTALTVLLSVIAATRARMAG
                     FTSATAVLLPGMVLSISVINDILHTNHFINTGNFTSVGLTVFVFGQAVLLGNQFTRSF
                     SQTRTLGRKLRAQKRTLSHFNTRLEEEVKTRTSELELARHKAESAANAKTMFLANMSH
                     ELRTPLSSIIGFSELLQREDLNPEESA"
     gene            complement(1438..2688)
                     /locus_tag="PKK76_10825"
     CDS             complement(1438..2688)
                     /locus_tag="PKK76_10825"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNL69011.1"
                     /translation="MIFYFLYNALLVLLWPIYQLLSLFVPRVRQFRLSRNEVEPQSFP
                     GSDGQPVFWLHAASVGEMDQALAIARELKRRGGCKIALSVFSLSVKKLQQPDVDFMFR
                     APVDFPWAWKRWVKRISPAAFVTMTWDVFPNLLASLRAARVPSYLACAALSHDSSRLK
                     FPLKQLLGSVYNKLSGIGAVDLENAERFRAIAGSTDVRITGDSRYDAIFHKIQTTKLD
                     SDTQRRLSSQGRTWILASTYRACDQKIFPELPALLRDFEDLRVLVFPHFVEEERLLEV
                     EEGLRAASIESRRFSDPDISGARVVIVDRMGILALAYRLADFCYVGGGFHHRIHNTGE
                     PAALGLPILTGTRIETSPIALLLEKEGALYRCEDGRQIIRHVRDFLDRKDYASSRGRA
                     GQKAIEQACGSSRKFVDAFLTPVP"
     gene            2705..3331
                     /locus_tag="PKK76_10830"
     CDS             2705..3331
                     /locus_tag="PKK76_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNL69012.1"
                     /translation="MQRPGSASAVKYFLFDLMDTIVVDPFHNLIANLLPPGMSFDEWR
                     SCRERAAFEDFEKGLISESEYFHRFYLQDLPESVKARLMRPQLLKTKLYEKIEFVHGM
                     HDLLVDLKGKATLAILSNYGPWYSEVLRLRPELEDLFDFLFFSCEIGHRKPEPGFYGA
                     VHEALHLEGRDAVFIDDREENLTEAARLGWKTHRFAGTKGLSQWISSL"
     gene            complement(3567..4805)
                     /locus_tag="PKK76_10835"
     CDS             complement(3567..4805)
                     /locus_tag="PKK76_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL69013.1"
                     /translation="MSEERPQEIDFASARDQIEKACDAAGELPAFTFEKGETRFYFSP
                     RNLDRAAVYNAMETVKAHIENVRVHTFESGFVVLQGMGDLYAPRAITSGIKFKFMSVG
                     GVPRIEVTRKGNFIQEEIQACIALFRNFHAAQGAGDPAAILGELGAQVYVPPSGSQDP
                     WTWMAGYEQTKSEVRESIVLPLKNGDLFREVARLARGSDQGVMPRAILFEGPPGVGKT
                     TMARIIATDTGIPLIYVPIENILSKYYGESAQNLSRIFDAAAMMKQAILFLDEIDSLA
                     GSRDEGMFEATRRVLSVLLRKIDGLDTVDGIITLGATNRSGDLDHALLSRFDHSIRFP
                     LPGREEIRAIYRTYAKHLKSEELIDLAAVSEGLSGRNIRDICEYAERRHARILLSTPG
                     SAASAPPASVYLEIARSRNV"
     gene            complement(4836..>5180)
                     /locus_tag="PKK76_10840"
     CDS             complement(4836..>5180)
                     /locus_tag="PKK76_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69014.1"
                     /translation="PGIRGVVLSQGGKTRTVDADRLLVKVGFEAENTLLSSGGHHFAH
                     AGSWLDSNDEQMALHSTGDMPLDSRPIWVAGDVCTPRDPSLAVAAGQACIAMRSIERY
                     LRSKPSLLPNRN"
BASE COUNT         1333 a         1350 c         1329 g         1168 t
ORIGIN      
        1 gcggactctt ccggattgag gtcttctctc tgaagcaatt ctgaaaatcc aatgatgctg
       61 ctgagaggtg tgcgaagttc gtgactcatg tttgccagaa acatagtctt cgcattggcg
      121 gcggactccg ctttgtggcg cgccagctca agctccgacg ttcttgtttt tacttcttct
      181 tcgagccttg tattgaaatg cgataaagtt cttttctgcg ctctgagctt tctcccaaga
      241 gttcgcgtct gagaaaagga acgagtgaac tggttcccca gcaaaactgc ctgaccgaaa
      301 acaaaaacag tcaacccgac tgaagtgaaa ttgccggtat tgatgaaatg gttcgtatgc
      361 aaaatatcat ttatgacgga aatagataga accatgcccg gcaatagaac agcagttgca
      421 gaagtaaaac cggccatgcg cgcacgagtg gctgcaatca cggacagtaa gacagtgagg
      481 gcagtgtatc cgttttgcag atagacagtc tgcgtataaa agctggccgg agtgaaaagc
      541 accatgatgc tcacaagaac ggaaagacca accatgcctc tcgtccagct gcgatgaaag
      601 aacgcaggaa aaagggagtt catatagagt gcgaagaatc cggaagacca ggtcaaagtc
      661 cagtattcga ctttgatgga taattcccac ggcagcgccg gaatcacaag caacgcgatt
      721 ttgcttccag tcactgtctc ccgtagggca atatcaatgc agaccaaacc aaagaagaag
      781 aagcttcgcc ttccgacaaa ccagaaggca acgtgataca tccctagaat cgcgagcatt
      841 gcaaatacta caatgctggt ccattgcttc agcacgatca ggtctgtgat tctgtttgcg
      901 tcggaaaacg aaatgctatg ccagattcca cccttgcgat agtgaaaatt ggaaacatgc
      961 aaaatcactt ccacctgatt tgaatgagga gtgaagatgt acgtggccgg ctgagtcaga
     1021 ggaacggata tctctggatt gtcagatgga aaaccgcgac ctcccaggag ctgaccttcg
     1081 catatcaacc tgtaggcggt gccgacttcg cctactctaa acgcgagagg ccgatctgtt
     1141 tgaaccagaa ttcgcatgcg aaaagagcca aaagcatgtg caccgagaga gtcgccattg
     1201 tacggagcct tgttccatga tccgggaacc gcgatgaaag aatggctgac tgccttaccc
     1261 gggctgatga attccatagg aaagaactcc cagtctccat ccagagcgat tggatccgtg
     1321 tttgcgaaat cgactccgcg caggtcgatc aaacctgcct gaacggaagg cggcgcagtc
     1381 accggacgac aggcgaagag aagtaagaga gcgggcagta cctttcgcac gtgttctcta
     1441 aggcacaggt gtgagaaagg cgtcgacaaa tttcctggaa cttccgcagg cctgctcaat
     1501 tgctttttgc ccggcccgac cacgcgatga agcgtaatcc ttacggtcta gaaaatcacg
     1561 aacgtgtcgt atgatctgac ggccgtcttc gcatcgatag agcgcacctt ctttctcaag
     1621 caacagtgcg atcgggctgg tttcgatcct cgtgcccgtc agaatgggca ggccgagagc
     1681 tgcaggctct cctgtattgt gaatgcgatg atgaaaccca ccgccgacgt aacagaaatc
     1741 ggcaaggcga taagcaagag ctagaattcc cattctatct acgatcacaa cgcgcgcgcc
     1801 cgaaatatct ggatcgctga atcttctgga ttcgatggaa gccgcgcgaa gcccttcttc
     1861 tacttccaga agcctttctt cttcaacgaa atgcggaaac acaagaaccc tcagatcttc
     1921 aaaatcgcgc aatagagcag gtaattcagg aaaaatcttc tgatcgcatg cgcgatatgt
     1981 agaagccagg atccacgtac gaccttgaga gcttagcctg cgttgcgtat ctgaatccag
     2041 ttttgtggtt tggattttat ggaagatcgc atcatatcga ctatcccctg ttatgcgaac
     2101 atctgtagac ccggcaatgg cgcggaatcg ttccgcattt tccaggtcca cagcaccaat
     2161 tcctgagagt ttattgtaca ctgatccgag caattgtttg agtggaaatt tcagacgaga
     2221 ggaatcgtgc gaaagagccg cacatgccag ataggatgga acgcgcgccg cacggagaga
     2281 tgcaagcaag ttcgggaata cgtcccatgt catcgtcacg aaggctgcgg gagaaatgcg
     2341 tttcacccag cgtttccagg cccagggaaa gtctacgggg gcgcgaaaca tgaagtctac
     2401 gtctggttgc tgcagtttct taacactcag gctaaatacg ctcagcgcaa tcttgcatcc
     2461 acctctgcgt tttagctcgc gcgcgatagc cagagcctga tccatttcgc ccacactggc
     2521 tgcatgaagc cagaatacag gttggccgtc tgatcccggg aaagactgcg gctccacctc
     2581 attgcgactc aatcgaaact gacgcacgcg cggtacgaat agagataaaa gctgatagat
     2641 cggccagagc agtactaaaa gcgcattgta gagaaaatag aaaatcatga aatcttcgtg
     2701 acgaatgcag cgtccaggtt ctgcttctgc cgtgaagtat tttctgttcg acttgatgga
     2761 cacgattgtg gtggacccgt tccacaatct gatcgccaat ctactgccgc cagggatgtc
     2821 ctttgatgaa tggcgatcgt gccgcgaaag agccgctttc gaagattttg aaaaaggact
     2881 gatcagcgaa agcgagtact tccacagatt ctatttacag gatttgcctg agtcagtgaa
     2941 agccaggctc atgcgccctc aacttctgaa aacaaaactc tatgaaaaga tagaattcgt
     3001 ccacggcatg cacgacttgc tcgtggatct aaaaggaaaa gccactctgg ccattctgtc
     3061 caactacggt ccgtggtatt cggaagtttt gcggcttcgt cctgagttag aggatctctt
     3121 tgatttcctg tttttttcct gcgagatcgg ccacaggaag ccggaaccgg gattctacgg
     3181 tgcagtgcac gaggcgctcc atctcgaagg gagagacgct gtgttcatag acgatcgtga
     3241 agaaaatctc acggaggcgg cccgccttgg atggaagacc catcgctttg ctggaacgaa
     3301 gggcctttcg cagtggattt caagcctttg aagcccatgg cgcccgaaaa aaagggcaaa
     3361 aaaagccccc ggggggaacc gggggcagga agagtgttca tgcgaagcat cggcctcctc
     3421 aaagaggagg cttcgggcgg ttggggggat gcccgaatta accgatgcca gtgccatgct
     3481 tttttcgtcg cctatgggcg caagcccgtt ttgcactgca caataaagaa aaaagaagcc
     3541 cgcggaggca gttcgagcca cgattgtcaa acattgcggg aacgagcaat ttccaggtaa
     3601 acggaagccg gtggtgcact cgcagcgctc cccggagtag aaagcaagat gcgcgcatgg
     3661 cgacgctccg cgtactcaca gatgtcccga atgtttctgc ccgaaaggcc ttcgcttacc
     3721 gctgccaggt caatgagttc ctctgatttc aaatgcttcg cgtacgtgcg atagatggca
     3781 cgaatctctt ctcgcccggg caaagggaat cggatcgaat gatcaaagcg agaaagcagg
     3841 gcatgatcca gatccccgga ccggttggtg gctcccagag tgatgatccc atcgaccgta
     3901 tcaagtccgt ctatttttcg cagcaataca gacagcactc gcctggttgc ttcgaacatt
     3961 ccttcgtctc tgcttcctgc tagagaatcg atctcgtcca gaaataggat cgcctgcttc
     4021 atcatggcgg ccgcgtcaaa gatgcgagaa aggttctgcg cagattctcc gtagtactta
     4081 ctgagtatgt tttcaatggg aacgtagatc aaaggaatgc cggtatctgt cgcaatgatg
     4141 cgagccatgg ttgtcttacc aacaccgggt gggccctcaa acaaaatcgc gcgcggcatt
     4201 acaccctgat cagaaccacg cgcaagacgc gccacttctc ggaatagatc tccattcttt
     4261 aacggaagaa caatgctttc gcgcacttca ctcttggtct gttcataccc tgccatccag
     4321 gtccacggat cctgacttcc cgaaggagga acatacacct gcgcgccaag ttcacccagg
     4381 atggcggccg gatctcccgc accctgcgct gcgtgaaagt tacgaaacaa cgcaatgcat
     4441 gcctgaatct cttcctgaat aaaatttcct ttgcgtgtta cttcgatgcg cggcacacca
     4501 ccaacgctca tgaatttgaa tttgattccg ctggtgatgg cgcgcggcgc gtaaagatct
     4561 cccattccct ggagcaccac aaaacctgat tcaaatgtgt gcactcgaac gttttcaatg
     4621 tgcgccttta cagtctccat cgcgttgtag actgcagcac gatccagatt gcgaggactg
     4681 aagtaaaacc tggtctcccc tttttcaaag gtgaatgcag gcaactcgcc tgctgcgtcg
     4741 cacgcttttt caatctgatc acgcgcggat gcgaagtcta tttcctgtgg ccgctcttca
     4801 gacatacgat ctacctgcct cgcccgcttt ccaggtcaat tccgattggg cagcaaggaa
     4861 ggtttcgatc gcagataacg ttcaatcgaa cgcattgcga tgcaggcctg tcctgctgcg
     4921 actgccagag aaggatcacg cggagtgcaa acatcaccgg ctacccagat gggcctggag
     4981 tccagtggca tgtctcctgt ggaatggagg gccatctgtt cgtcgttcga atccagccag
     5041 ctgcctgcgt gtgcaaaatg atgacctccg gaagaaagaa gagtgttttc tgcctcaaat
     5101 ccaactttca caagaagccg atccgcatcc acagttcgcg ttttgccgcc ctggctgagc
     5161 acaacacctc tgatacctgg
//
LOCUS       DAORFF010000251         5102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9167_length_5102_cov_3.862294, whole genome shotgun sequence.
ACCESSION   DAORFF010000251 DAORFF010000000
VERSION     DAORFF010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9167_length_5102_cov_3.862294"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..970
                     /locus_tag="PKK76_10845"
     CDS             53..970
                     /locus_tag="PKK76_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL69015.1"
                     /translation="MRRTVAILASVGIVLAVLPFACDGERHAITEQDRAGKPGQFAGL
                     SQGVTHYEIAGPAKGRPVLLVHGFSVPYFIYDFVFEALVQKGFRVIRLDLYGRGLSDR
                     PDVVYDLDLYDRQLTDLLDYLKISRVHIVGSSMGGLITANFLRKHPERVSRAVFIAPA
                     GFPMPLPISAKIGRAPYIGDYIVRVAGDRIFVKNNSASFFGNVPPGFYERFGEQMQFK
                     GYKRALLSSLRNMPLESGASIFDEAGRKKIPSIVIWSRQDGVLPYSNAIRAQYSLGAS
                     ELISLEGTGHTPHFENPQMVIPHILKFFQ"
     gene            1075..2907
                     /gene="glmS"
                     /locus_tag="PKK76_10850"
     CDS             1075..2907
                     /gene="glmS"
                     /locus_tag="PKK76_10850"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNL69016.1"
                     /translation="MCGIVAYLGPKSVDSVLLVGLTRLEYRGYDSAGIAVLDNGEITV
                     RKEKGKIKDLEDLLLTHPVEGTIGIGHTRWATHGEPNRQNAHPHVDNHGKVAVVHNGI
                     IENHHTIRQELIEKGHVFHSETDTEVIPHLFEDYYKKHKDSAKALQETIARLDGRYAF
                     VVLVEQEPDRLYFARSGSPLVYGRGRGESFLASDVSAIVPAAKEHLHIDNDQWGWITK
                     DSLELRSKTGEAVPFEMSRTQIDVKALDRAGYDHFMLKEIHEQPGIVRRIIQSRMDDE
                     GIVRFPEMNLPNDFLNRVTRCMITSAGTSWHAGIVGKFYLEQFARLSTEVDISSEFRN
                     RNPIADGETLVMAISQSGETADTLAGIHQAKAKFCRVLSFVNVTTSSIARESNAFIDL
                     MAGPEIGVASTKAYTAQLMNLLFYALFTAGIKWLVSKEEREQILHEIRLLPEKMEQIL
                     ARADEIKKWASELRDSKDFVFLGRSFNHPTALEGALKLKEIAYVHASGYAGGEFKHGP
                     IALISDQMPVICIAPQCDLYEKMVSNIEEVRSRKGRIISIYTEGDTIVPSKSDYSFAI
                     PQCADYLTPILTVLPLQLFAYYSALERGCEPDQPRNLAKSVTVE"
     gene            3095..4009
                     /locus_tag="PKK76_10855"
     CDS             3095..4009
                     /locus_tag="PKK76_10855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HNL69017.1"
                     /translation="MEARGADSIAFQDSPLRSEFPRTPHEIENGALAKGISFVRARPG
                     SLFSRLDSIGESIKNDLPVYLARSGDSVKAPDHAQVIGPSDALHIHATATIHPGVIFN
                     TEDGPIVVDAGAKISPFSYLSGPLYVGPRAQLDHLKIANSIVGEGCRLGGEIESSIFG
                     DFTNKHHEGFVGHSLIGNWVNLGALTTTSDLKNNYGKIRLHLPGAVVETGRIKFGSIL
                     GDCVKTAIGTMLNTGSVVDAGSSVFGGRPPAYLGPLSWGLTGQTYELDRFLKDCETIF
                     ARRSQTVPAAMRRMVELSVSLDAGYTGS"
     gene            4017..4295
                     /gene="xseB"
                     /locus_tag="PKK76_10860"
     CDS             4017..4295
                     /gene="xseB"
                     /locus_tag="PKK76_10860"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNL69018.1"
                     /translation="MAQKKKPTFEEALSELEEITRLLESGELTLDQSVEAYEKGMELK
                     KACYEMLSTAEKKLEYLEKKDGELKRVALASENMRADNRLFEDDDSED"
     gene            4296..>5102
                     /locus_tag="PKK76_10865"
     CDS             4296..>5102
                     /locus_tag="PKK76_10865"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNL69019.1"
                     /translation="MRFLLILAALLMASSCRTPPGCSSTFCDYKDELTVYGRNVHLLY
                     EDNFREISSNCAFRTELTTGDYVPMTQQQALTILRNMAGEMGGNALVFQSFRKSKDAS
                     GRVYVCSNFGSATSGQVQSNQISTQSTTQTQSQVSSVVIQPDTRVTSSINTRQNTTPT
                     NTQQQNVNLNQVQPIQPVSQAMTVNDTGRKLSWQTCSMGQSYSGGKCSGQAVAETFQS
                     ASSYCSSQSTAGYTYRLPDRIELASLLTSTGTPKADPRVFPDTKPDIYWTR"
BASE COUNT         1276 a         1238 c         1309 g         1279 t
ORIGIN      
        1 tcatgaacca gcagcgtgac gtggttctcg aatctgcccg ggagatggaa cagtgaggcg
       61 gaccgttgcg attctagctt ctgtgggaat cgttctggcc gtattgcctt ttgcctgtga
      121 cggagaacgt catgcgatca ctgaacagga tcgagcggga aagcccggtc aatttgccgg
      181 cctttcgcag ggagtaactc actacgaaat agccgggccg gcgaaggggc gtccggtgct
      241 tctggttcac ggcttttcag ttccttattt catctatgat tttgtttttg aagctcttgt
      301 tcaaaaagga tttcgtgtga tccgcctgga cctgtacggg cgcggacttt cggatcgccc
      361 tgacgttgtc tatgatcttg atctctatga caggcagctg acggatctcc tggattatct
      421 caagatttcg cgcgttcaca ttgtaggatc ttccatgggg ggactcatca ccgcgaactt
      481 cttaagaaag catccagaga gagtttcccg cgcggtgttc attgcgcctg cgggctttcc
      541 tatgccactt cctattagcg cgaaaattgg ccgcgcgccc tatattggag attacattgt
      601 gcgcgtggct ggagatcgca tcttcgtaaa gaacaacagt gcaagttttt ttggcaatgt
      661 gcctcctggt ttttacgaaa ggtttggaga acagatgcaa ttcaaaggat acaagagagc
      721 tctgctgtct tccctgcgaa acatgccact ggaaagtgga gcgtccattt tcgatgaagc
      781 agggcgaaag aagattccgt ctatcgttat ctggtccagg caggatggag ttcttcccta
      841 ttccaacgcg attcgcgcac agtattcgct cggagcgtca gaacttattt cgctcgaggg
      901 cacggggcac acgcctcact ttgaaaatcc acagatggtg attccgcaca tcctgaagtt
      961 cttccagtag cacgttgaaa tttctccaca tggttgcaga cccgcaacga ctcgagaaga
     1021 tcgctccctt gaatcggact tgcttcacgg agtccttcta attttgtttc actcatgtgc
     1081 ggtattgtag cgtatctggg accgaagtca gtcgactcgg ttcttctagt cggtctaacg
     1141 cgtcttgaat accggggtta cgattccgcc ggcatcgccg ttctggataa cggtgaaatc
     1201 acggtcagaa aagaaaaagg aaagatcaaa gatcttgaag atttgctgct cacgcatcca
     1261 gtggaaggga ccattggaat tggacacact cgctgggcta cgcacggaga acccaatcgc
     1321 cagaatgcgc atccgcatgt tgacaatcac ggaaaagtgg cagtcgttca caatggaatc
     1381 atagagaatc atcacacgat tcgtcaggag ctgattgaaa agggtcacgt ctttcattct
     1441 gagacagaca cagaagtgat tcctcatcta tttgaagatt attacaagaa gcacaaggat
     1501 tctgcgaagg ctcttcagga aaccatcgcg cgcctggatg gccggtacgc attcgtggta
     1561 ctggtagagc aagagccgga tcgtctctat ttcgcacgaa gcggttctcc tcttgtctat
     1621 gggcgcggaa gaggagagag ttttcttgca agcgacgtgt ccgccattgt gccggcagcc
     1681 aaagaacacc tccacataga caatgatcag tggggctgga ttacaaaaga tagtcttgaa
     1741 cttcgtagca aaacaggtga ggcagttccg tttgaaatgt ccagaactca gatcgatgtc
     1801 aaagcactcg atcgtgctgg atacgaccat ttcatgctca aggaaatcca tgagcagccc
     1861 ggcattgtaa gaagaattat tcaaagtcgc atggacgatg agggaattgt tcgattccct
     1921 gaaatgaatt taccaaacga ttttctcaat cgtgttacgc gctgcatgat tacttccgct
     1981 ggaacgtcct ggcatgcggg gatagtagga aaattctatc tggagcagtt tgcgaggctt
     2041 tccactgaag tggatatttc ttctgaattt agaaatcgca acccgattgc ggatggggaa
     2101 accctcgtga tggccatatc tcagagcggg gagacagcag acacgttagc cggcattcat
     2161 caagcaaaag caaagttctg tcgagttcta tcttttgtca atgtaaccac atctagcatt
     2221 gcgcgcgaat ccaatgcgtt cattgatttg atggctggtc cggagatcgg tgtggcctct
     2281 accaaggcat atacggcgca attgatgaat cttctattct atgctctctt cactgcaggc
     2341 ataaagtggc tcgtgagcaa ggaagaacgt gagcagatcc tgcacgagat ccgtctcctt
     2401 cctgagaaga tggaacagat tctggcgcgt gcagatgaaa tcaagaaatg ggcgagcgaa
     2461 cttcgtgatt ccaaagactt tgttttcctg ggccgatcgt tcaaccaccc gactgctctc
     2521 gaaggggcac tgaaactcaa agaaattgca tatgtgcatg catcaggata tgcgggcgga
     2581 gagttcaagc atggacctat cgctttgatt tcagaccaga tgcctgtgat ctgcattgca
     2641 ccacagtgtg acctttacga aaaaatggtt tcaaatattg aagaagttcg ttcgaggaaa
     2701 ggccgcatca tttccattta cacagaaggg gacacgattg ttccttcgaa gtctgattat
     2761 tcatttgcga ttccacaatg cgcggattac ctcactccaa tcctgacagt attgccattg
     2821 caactgttcg catattattc tgcgctcgag cgcgggtgcg agcctgatca gccgcgcaac
     2881 ctcgccaagt ctgttacggt ggagtaagac catatcttac gttatcctaa tcgattcggg
     2941 tagtccagaa acactccctc tggcgcgctt tcgttctgtc ttctctctac gagatggaat
     3001 ctattcacct ctgactcgag cgcgaattgc tcatccaaca gccaggctgg ttttttttca
     3061 tccggctttt tcttacgaaa aggcagtctg cgaaatggag gctcgtggag cagacagcat
     3121 tgcatttcaa gattctcctc ttcgctctga atttcctcga acaccgcatg agatagaaaa
     3181 cggtgcactt gcaaaaggaa tctcttttgt tcgcgctcga cctggttctc ttttttcgcg
     3241 tttggattcc ataggcgaat ccataaagaa tgacctgcct gtatatctgg cacgcagtgg
     3301 cgattccgta aaagcacctg accatgcaca ggtgatcgga ccgtcggacg cgctccatat
     3361 tcacgcaact gctacaattc atcccggtgt aattttcaac acagaggatg gtcccatcgt
     3421 tgtggatgca ggagcgaaga tctctccatt cagctatctt tcaggcccat tgtatgttgg
     3481 gccgcgtgcg caattggatc atttgaagat tgcaaattcg atcgtgggcg aaggttgcag
     3541 gcttggcgga gaaatagaat cgtctatctt tggtgatttt acaaacaaac accacgaggg
     3601 ttttgtgggt cacagtttga ttggcaactg ggtgaatctg ggtgcgctca ccaccacatc
     3661 cgatttgaaa aacaattacg gcaagatcag attgcatctg cctggagcag tggtggaaac
     3721 agggcgaatc aaatttggct ccattctggg cgattgcgtc aagactgcga ttggcacaat
     3781 gctaaacaca ggatcagttg tggatgcagg atcgagcgtg ttcgggggga ggcctccggc
     3841 gtatctcggg ccactttcct ggggacttac cggtcagaca tatgaactgg atcgcttcct
     3901 caaggattgc gaaacaattt ttgctcgccg ctctcagact gtgccagccg ccatgcgccg
     3961 tatggtagag ctgtccgttt cacttgacgc cggctacacc ggaagctgaa tccaacatgg
     4021 ctcaaaagaa aaagcctacg tttgaagaag cactgtcgga gctagaggag atcactcgtc
     4081 tactggagag tggggagttg acgctcgacc agtctgtgga agcatatgag aagggcatgg
     4141 aactgaaaaa ggcctgctat gagatgcttt ctacggctga gaaaaagctc gaataccttg
     4201 aaaagaagga tggggagttg aagcgtgtag ctctggcgtc cgaaaatatg agagcggata
     4261 atcgtctttt tgaagacgac gattcggagg actgaatgcg attcttacta atcctcgcgg
     4321 cgcttttgat ggccagttcg tgcagaactc cgcccggctg cagcagcact ttttgcgatt
     4381 acaaggacga actgaccgtc tacggacgca acgttcatct tctatatgaa gataacttcc
     4441 gcgagatttc gtctaactgt gcatttcgca cggaactcac gacaggtgac tatgtgccta
     4501 tgacccagca acaggccctg accatcctgc gaaacatggc gggagagatg ggcggaaatg
     4561 ctcttgtttt tcaatcattt agaaaaagta aggatgcgtc cggtcgagtc tacgtctgtt
     4621 ccaatttcgg aagcgccact tcgggccagg tgcagtccaa tcagatctcc actcaaagca
     4681 caacgcaaac tcagtctcag gtcagctctg ttgtaatcca gcctgatacg cgtgttacat
     4741 ccagcatcaa tacaagacag aacacaactc cgaccaacac ccaacagcag aacgtcaatc
     4801 taaaccaggt tcagcccatc cagccagtct cccaggcaat gactgtgaat gatacaggaa
     4861 gaaagctcag ctggcagacc tgcagcatgg gtcagagtta ctctggcgga aaatgctcgg
     4921 ggcaggcagt ggcggaaaca tttcaatccg cttcctcgta ttgcagttcg cagagcacgg
     4981 ccggctatac gtatcgcctg cctgatcgca ttgaactagc ttctcttctg acttctaccg
     5041 ggacgccgaa ggcggatcca agagttttcc cggacaccaa gccggacatt tactggacca
     5101 ga
//
LOCUS       DAORFF010000252         5074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9256_length_5074_cov_4.417015, whole genome shotgun sequence.
ACCESSION   DAORFF010000252 DAORFF010000000
VERSION     DAORFF010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9256_length_5074_cov_4.417015"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PKK76_10870"
     CDS             complement(<1..923)
                     /locus_tag="PKK76_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNL69020.1"
                     /translation="MSQAKIGPIRTYIRDISELPSYLKRLWLHLKPVRRTQVFLLMGL
                     MVAVSAAEVISIGAILSFLAVLTDPGRTFHHPRLQFLWELTGIQSPEQMVLPMTIAFA
                     CAALVTGGARLLFLWANTRLCNAIGADLAYDVYRRTLFQPYFVHVGRNSSEVATATSK
                     AKGLVNQTISPLLTIFGSLCVIVSIVSFLVTIDPVVSLSAVAGFSTIYFVIAIFSKNS
                     LDTNGRILARELTRATQTMSEGLGGIRDILLDGSQDFYSGIFRTSDRRLNRANANATF
                     LAGTPRFAIEAVAMVLLAGFAYALTRSPGGL"
     gene            complement(910..1863)
                     /locus_tag="PKK76_10875"
     CDS             complement(910..1863)
                     /locus_tag="PKK76_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNL69021.1"
                     /translation="MEKNAKIYIAGHRGLVGSAIVRVLAEQGYTNVMGRTHSELDLTR
                     QPDVDAFFSKEKPDYAILAAAKVGGIHANNTYPAEFIYSNLQIQNNIIDACYRHGVKK
                     LLFLGSSCIYPKFAHQPMSESELLNGKLEPTNEPYAIAKIAGIVMCQSYNRQYGTNFI
                     SAMPTNLYGPGDNYHPENSHVIPALIRRFHEAKKNNVKEVVIWGTGSPLREFLFSDDC
                     ARACLFLMERYDVRGDSTGGEIVNIGSGSEISIRDLAKTVGEVVGFRGDIIFDVTKPD
                     GTPRKLLDVAKLHKMGWRHTVELREGLATAYHDFEAAHVSG"
     gene            complement(1869..2912)
                     /gene="fba"
                     /locus_tag="PKK76_10880"
     CDS             complement(1869..2912)
                     /gene="fba"
                     /locus_tag="PKK76_10880"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775027.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HNL69022.1"
                     /translation="MALVPMRLLLDHAAENNYGIPAFNVNNLEQVLAIMRAADRADSP
                     VILQVSRGARAYAGEHFIRHLILAAVESYPHIPISLHQDHGNSPATCFSAIRNGFTSV
                     MMDGSLKEDGKTPADFEYNARVTADVVRVAHSIGVSVEGELGCLGSLETMTGDKEDGH
                     GAEGKLTREQLLTDPDEAVAFVQKTNVDALAVAIGTSHGAYKFKQKPTGEVLVIKRIE
                     EIHKKLPNTHIVMHGSSSVPQDYLAIINKTGGKIAETYGVPVEEIQKGIKVGVRKINI
                     DTDLRLGWIAAVREAAAKKPEEFDPRYFLKPAMKNMEDVCADRFAKFWCAGNGSKIKQ
                     VGLDEYAKKYSAG"
     gene            complement(2914..3447)
                     /locus_tag="PKK76_10885"
     CDS             complement(2914..3447)
                     /locus_tag="PKK76_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNL69023.1"
                     /translation="MRAILLDRDDTINEDPGYLADATQVRLLPRVVEGLSLLRSHGYR
                     FFVMSNQSGVARGLISGPELQAVNDRISELLLAQGIVIERFYVCPHRDEDGCDCRKPK
                     PGLFRQFFQDWNASGSQCFAIGDKPRDLEAARPFGVRGILLQAGKEAPVYFPKNLVYR
                     AVDLLDAADYIIRTEGD"
     gene            complement(3444..3956)
                     /locus_tag="PKK76_10890"
     CDS             complement(3444..3956)
                     /locus_tag="PKK76_10890"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNL69024.1"
                     /translation="MHDIERLSEGEARLIEALAERSSSQRILARAAGLSLGMTNILIK
                     RLVKKGLIKVVTLNGRTLSYMLTPRGFAEKVKRTYEYITVSIRFIDRVRSNILSIVAA
                     LPEGSPLYIAGPGELAQLAAETLRESGRVFEQVNTVADLEGKTGVCLVCLPEMPVPIP
                     PGLILKSIVA"
     gene            complement(3969..5030)
                     /locus_tag="PKK76_10895"
     CDS             complement(3969..5030)
                     /locus_tag="PKK76_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HNL69025.1"
                     /translation="MYAPPLKMKMLVTGSAGFIGFHLVERLLSEGHQVVGIDNLNPYY
                     DPALKVSRLRAAGIEDPVPGSIVHARQRSYGFLQGDITNSTDLKSVFDLGPFDCVCHL
                     AAQAGVRFSLEQPLAYVHANIVGTTHLLEACRSDAPHFLFASSSSVYGLNQKMPYSVH
                     DNTDHPVSIYAASKKASELIVHSYSHLFGIPSTGLRFFTAYGPWGRPDMALFKFTQSI
                     LKGEPIQVYNNGRMQRDFTFIDDIVTGVVKLAHHIPQPDEGWDANHPDPSTSSAPYRI
                     HNIGNSRPTELLDYVRAIERALGKQAVLEMLPAQPGDVTSTWADTSELQKEIDFIPDT
                     AIDVGVSKFVAWFRQYYNV"
BASE COUNT         1266 a         1341 c         1278 g         1189 t
ORIGIN      
        1 ttgagacctc ctggagagcg cgtgagcgcg taggcgaagc cagcaagaag aaccatggcg
       61 acagcttcaa tggcaaacct gggcgtcccc gcaagaaaag tggcatttgc gtttgcacga
      121 ttcaaacgcc tatctgacgt gcgaaaaatg ccactgtaga aatcctggct gccgtcgaga
      181 aggatgtcgc ggataccgcc aagaccctcg ctcatagttt gagtggctcg agtgagttcg
      241 cgagccagaa tacgtccatt cgtatccaga ctattctttg aaaaaatagc aatcacgaaa
      301 tatatcgttg agaaccctgc gacagcggag agtgaaacga cagggtctat ggttacaaga
      361 aaagaaacga tggaaacaat gacgcagagg gatccaaaaa ttgtgagaag cggcgaaatt
      421 gtctgattca cgagaccttt cgccttcgat gtcgcagtcg ctacttcact ggaattgcga
      481 cccacgtgca caaaatacgg ttggaacaaa gtgcggcgat acacatcgta cgcaaggtcc
      541 gcaccgatcg cattgcagag ccgcgtattc gcccacaaaa acaatagacg tgcgccacct
      601 gtcaccaggg cggcacatgc aaaggcaatc gtcataggaa gaaccatttg ctccggcgac
      661 tgaatcccgg tcagttccca taggaattgg agccgaggat ggtgaaacgt tcgccctgga
      721 tctgtgagga ctgccaggaa ggagaggatc gctccgatgc tgatcacttc ggcggcggat
      781 acagcgacca tgagccccat caagaggaac acctgagttc tgcgaacagg cttcagatgc
      841 agccagagtc gcttcagata agagggaagc tcggagatgt cgcgaatata ggtccggatc
      901 gggcctatct tagcctgaga catgtgctgc ctcaaagtca tgatatgcgg tcgcgagtcc
      961 ttcgcgcaat tctactgtat ggcgccagcc catcttgtgc agcttcgcaa catctaacaa
     1021 tttcctggga gtcccgtccg gctttgttac atcgaatatg atgtcaccgc gaaagccaac
     1081 gacctccccc acagtctttg cgagatcccg tatggaaatt tctgacccgg atccaatgtt
     1141 cacaatttcg ccgcccgttg aatctccgcg aacatcatat cgctccatca agaagagaca
     1201 tgcacgcgcg caatcgtctg aaaaaagaaa ctcgcggaga ggcgagcctg tcccccagat
     1261 gactacctct ttgacattgt tcttctttgc ctcatggaac ctgcgaatca gcgccgggat
     1321 gacatgcgaa ttctccggat gatagttatc tccaggtccg taaagattcg taggcatagc
     1381 agagatgaag ttcgtcccgt attgacggtt gtagctctga cacatgacta ttcccgcaat
     1441 tttcgcaatc gcatagggct catttgttgg ctcaagcttt ccattcagca actcagattc
     1501 gctcatgggt tgatgagcga actttggata gatgcacgaa gagccaagga ataagagctt
     1561 ttttacgccg tgacggtaac atgcatctat gatgttgttc tgtatttgaa ggtttgaata
     1621 aataaactct gcgggatacg tattattcgc atgaatccct ccgaccttcg ccgcggccag
     1681 aatagcatag tccggctttt cttttgaaaa aaaagcatcc acgtcaggtt ggcgagtcag
     1741 atccaattcg gaatgggtac gacccatcac gttcgtgtaa ccttgctcgg cgagaacgcg
     1801 aacaatcgcg gagccgacga gtccgcgatg acctgcaatg tatatctttg cgtttttttc
     1861 cattcgtttc agcctgcgga atattttttc gcgtattcat ccagtccgac ttgcttgatc
     1921 ttcgaaccat tgcccgcgca ccagaattta gcgaaacgat ctgcgcaaac atcttccatg
     1981 ttcttcatag cgggtttaag aaagtatctt gggtcaaatt cctcaggttt cttggccgca
     2041 gcttcgcgca cagcagcaat ccaacccagg cgaagatccg tatcgatatt gatcttgcgc
     2101 actccgacct tgatcccctt ctgaatttct tccaccggga caccgtaggt ttccgcgatc
     2161 tttccacctg tcttgtttat gatggcgaga tagtcttgcg gcacactgga agaaccatgc
     2221 ataacgatgt gtgtatttgg aagtttctta tgaatttctt ctatgcgttt gatcacaaga
     2281 acttcgccgg ttggtttctg tttgaacttg taagctccgt gactcgtgcc aattgcaacc
     2341 gcaagtgcat ccacattcgt cttttgaaca aacgcaactg cttcatcagg atccgtcagt
     2401 agttgctcgc gcgtaagttt cccttccgct ccgtgcccat cttctttgtc gcccgtcata
     2461 gtttccagag aacccagaca tccaagctcg ccttctacgc tcacaccaat ggaatgcgca
     2521 acgcgaacca cgtccgcggt cacgcgcgcg ttgtattcga aatcggcggg agttttgccg
     2581 tcttctttca gggaaccatc catcatgacg gaagtgaatc cgttgcggat ggcggagaaa
     2641 caggtggccg gactgttacc gtgatcctga tgtagcgaaa taggaatatg aggataggac
     2701 tcgacagcag cgagaatcaa atgtcgaata aaatgctctc cggcataggc ccgcgcgccg
     2761 cgcgaaactt gaaggatgac aggactgtcc gcccggtccg ccgctcgcat gatggcgagc
     2821 acctgctcca gattatttac attgaacgcg ggaatcccgt aattgttttc tgcagcgtga
     2881 tcgagcagca gcctcatcgg tacaagcgcc atactagtct ccttcagtcc tgattatgta
     2941 gtccgccgca tccagaagat ccacggccct gtagacaaga tttttcggga aataaacagg
     3001 cgcctctttt ccggcctgca aaagaatccc acgaacgcca aatgggcgag cagcttccaa
     3061 atcgcgtggt ttatcgccga ttgcgaaaca ctgagaacct gacgcgttcc aatcctggaa
     3121 gaattggcga aacaggccag gctttggctt tcgacaatcg catccatctt catcgcggtg
     3181 cggacaaaca tagaagcgtt cgattactat tccttgagcg agcaacaatt ccgagattcg
     3241 atcgttgacc gcctgcagct ctggcccact gattaagcct ctggctaccc cggattgatt
     3301 gctcattacg aaaaaccgat aaccatgaga acgcaacaga ctcagcccct ccactacgcg
     3361 cgggagtaac cgaacctgag tcgcatctgc gagatagcca gggtcttcgt tgattgtatc
     3421 gtcccgatca agcagaatgg ctctcatgca acgatgctct tgagaatcag tcccggagga
     3481 atggggacag gcatttccgg aagacacaca agacaaactc cggtcttacc ttccaaatcc
     3541 gcaaccgtgt tcacctgctc aaaaacgcgt ccggattcgc gcaaagtctc agcagccagc
     3601 tgcgccagct cacccggacc cgcgatgtaa agaggcgaac cttccggcag agcagctacg
     3661 atcgaaagaa tgttacttcg cacacgatcg atgaatcgta tggagacggt aatgtactca
     3721 tacgttctct ttaccttttc cgcgaaacca cgcggcgtga gcatgtaact tagagtccga
     3781 ccattcagag tcaccacttt gatcagtccc ttctttacaa gacgcttgat gagaatgttt
     3841 gtcatcccga gggacaaacc tgcggcccgt gccagaatcc gctgactgct gcttctttcg
     3901 gcgagcgctt ctatgagccg cgcttcacct tcgctcaatc gttcaatgtc atgcatgatg
     3961 gagagaaact aaacgttgta gtattgcctg aaccaggcga caaacttgct gacgccaacg
     4021 tcaatcgccg tatcgggtat gaagtcgatt tccttctgta gttcagaagt gtctgcccag
     4081 gtagacgtca catcgcctgg ttgcgcagga agcatctcaa gcactgcctg tttaccgaga
     4141 gctcgctcaa tggcgcgaac atagtcgagc aattctgtcg gcctggaatt cccaatgtta
     4201 tgaatgcgat atggcgccga agatgtagag ggatccggat gattcgcgtc ccagccttca
     4261 tctggttgag gaatgtggtg cgccagcttc accactccgg tcacgatatc atctatgaaa
     4321 gtaaaatcac gctgcatgcg cccgttattg tacacttgaa ttggctcgcc cttcaggatc
     4381 gactgtgtga acttgaagag agccatatct ggcctgcccc agggcccata cgcggtgaag
     4441 aatcgaagac ctgtggaagg gatgccgaac aggtggctgt acgaatggac tatgagctcc
     4501 gatgctttct tactggcggc atagatactc actggatgat ctgtgttgtc atgaactgag
     4561 taaggcattt tttgattcag tccgtaaacg gaggaactgg aagcgaatag aaagtggggc
     4621 gcgtcagatc ggcaggcttc tagaagatgg gtcgtgccca cgatgttggc atgcacatag
     4681 gccaggggtt gctcaagact aaatcgtacg cctgcctgcg ctgccagatg gcagacgcaa
     4741 tcaaacggcc caagatcaaa tacggacttc aaatcggtcg aatttgttat gtccccctgc
     4801 aagaagccat acgaccgctg tctggcatgg acgatcgacc ctggcactgg atcctcaata
     4861 cctgcggccc tcaagcgact gactttgaga gcgggatcgt agtaaggatt caagttgtct
     4921 attccaacaa cttgatggcc ttcggagagc agccgctcca cgaggtggaa tccgataaac
     4981 ccggctgaac cggtgactag cattttcatc ttaagagggg gcgcgtacat ggcttcgcca
     5041 ggtgagtccc agggggaaaa aaggcacctt ccag
//
LOCUS       DAORFF010000253         5073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9262_length_5073_cov_3.854922, whole genome shotgun sequence.
ACCESSION   DAORFF010000253 DAORFF010000000
VERSION     DAORFF010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9262_length_5073_cov_3.854922"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="PKK76_10900"
     CDS             complement(<1..1244)
                     /locus_tag="PKK76_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69026.1"
                     /translation="MNYSAAGKKVLKAIATDILIMRPRGYVLDIISPIPSDLAEQELA
                     NRIKTDLKDEAGIALFNLDTPEIREVDGIVWSPTLGFFLLETKGFQIEDFKAISLVAI
                     NYARENHRRKYGHKPPPWKGAKATARKLASYFDSLYFGRRIFPEAPEPRAQFKDLHIP
                     YISFAVFFPFIREEEFRDRFPQTYPVLEKILIFKDSPLRSKLEAIAHALAPGDKKKGG
                     GDHAEALRVINEYKRKVFRFAPEEAQQSKYDWERLNRLENTEIQADLRHLSLKHPAHR
                     YGYAGTGKTVIGLALLRNLALGGKRVLYTCFNKVLASDFRRWNRCAGVIEYKYLSQID
                     FKDIHQIITELEPFQLRVQRHEEPRDLFSKDDFQAHFERVVATVIADGGYRGVYDYIV
                     IDEGQDLNDYAWRLLEPLSVDG"
     gene            complement(1380..1517)
                     /locus_tag="PKK76_10905"
     CDS             complement(1380..1517)
                     /locus_tag="PKK76_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69027.1"
                     /translation="MAKSMIQELRDGKKKNPGIEVFTRLLKPLGARLVIGDGKREVTV
                     G"
     gene            complement(1674..2384)
                     /locus_tag="PKK76_10910"
     CDS             complement(1674..2384)
                     /locus_tag="PKK76_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNL69028.1"
                     /translation="MFEQEKESFHKIKAIAKPFLDWMVDIQHEGLEYVPREGGCILVG
                     NHRSDMDPFIVASVVPRFISWIAAEYTQRIPVFKNLVRETGVIPMQIDGNVSVASIKK
                     TMSVLKSGEILGIFPEGHDYMVKNQFSSPMVDFHQGFATFAARGKAPIIPFVIIPIEE
                     DLETIPVPGYVRSLFGLPEEVCKIPHRVVYKKVKIIFCEPVMYNAFQDVPGQDSILVH
                     HVRSAMEERLTAHGLTQR"
     gene            2412..3215
                     /locus_tag="PKK76_10915"
     CDS             2412..3215
                     /locus_tag="PKK76_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69029.1"
                     /translation="MSIMYPGLLLAFVSTFARVKDHYGRVFVLGRNAEVTMDVKHGGW
                     KPVLPVVEDAILHLAEADALRREGNLEEAVCLLRAIEVMGTGPSKDATRRLENIDRSR
                     AVKDLLAQCDPIVYFRKQTGRTTVQSDTAGLKVSFLGEFRIMKAKSVDSPTLTQKIIY
                     LGHDAESILIAYDLWPRSVSVEDYRRIWDKRRGITSTSTLSDLNAGICWPGRIEAGGP
                     TTAQCAIYEMVEGKQGIEYYETGRLKGIFLAFSGKDRARDALRELRITK"
     gene            3212..4591
                     /locus_tag="PKK76_10920"
     CDS             3212..4591
                     /locus_tag="PKK76_10920"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL69030.1"
                     /translation="MRTSLRILRFLRRYSTAFLHPDHPFLRDYQERRRLQIIMVTGGL
                     LGVFMLIRGILVIRDTPEIGGIVLTLAFLIFAGMSLIVALRAKGYRWIALALVTVAGG
                     ANYANSYLSRVFPPAGLPFAAAVVVALHFLFNRWMSLLGLVVQIVAFLPFLYQQDFHY
                     ASEGERQFWHTRFFANSAVSLGIIWILCEAYRSVRDQAEIRLNRLKAELARDITLAGK
                     IQKDLLPPNKSEGGPYQWHGFMRTMSGAGAGGDYIDALEIRGHTWFAMGDVTGHGIQA
                     GLLVMLARTSLHQVLRDQQVQDPGKVLSLLDYSFSRTASSLTEKSFMTFVLMRFDANG
                     KVTFAGSHLKILVYRPAEDRMDILETKGPWIGVLTADRRVSFDEFTFQLGIGDIVLLY
                     TDGITEARDALGEAIEVKGLLNMFREALQESQDIEKINHRIIDLLVHATGKNIFDDDL
                     SLLTIRRAH"
     gene            4603..>5073
                     /locus_tag="PKK76_10925"
     CDS             4603..>5073
                     /locus_tag="PKK76_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNL69031.1"
                     /translation="MTRKERDTMGEIEVASDRYWGAQTQRSLHHFKIGHDHFPREFIR
                     AMGILKKAAAIANRELGLLSQEKVNLIMRAADEVIDGKLDDHFPLVIWQTGSGTQTNM
                     NANEVISNRAIELAGGKMGSKDPIHPNDDVNKAQSSNDTFPTGMHIAAAERIAQH"
BASE COUNT         1243 a         1315 c         1278 g         1237 t
ORIGIN      
        1 cagccgtcca ccgaaagggg ttcaagcaac cgccatgcat agtcgttgag gtcctgtcct
       61 tcatcgatca cgatgtaatc gtagacacct cgataaccac catcggcaat caccgtagcg
      121 acaactcgct caaagtgcgc ctgaaaatca tctttgctaa acagatcgcg cggttcctca
      181 tgacgttgga ccctcagctg gaatggttca agttcagtga tgatctggtg gatgtcctta
      241 aagtcaatct gagacaggta tttatactcg atgacccccg cgcatcgatt ccagcgtcta
      301 aagtcgctgg ccagaacttt gttgaagcat gtatatagca cccgtttccc ccctagcgcg
      361 aggttgcgga gcaaggcaag accgatgact gtcttgccgg tgcctgcata gccataacgg
      421 tgggccggat gtttgaggga caaatgtctc aggtctgctt ggatttcagt gttttccagt
      481 ctattgagtc tctcccaatc atacttggac tgctgagctt cctcgggggc gaaccggaag
      541 actttgcgtt tgtactcgtt aataacgcgg agtgcctcag catggtctcc accgcccttc
      601 ttcttatcac ccggcgcgag agcatgggcg attgcttcga gcttggatcg taacggactg
      661 tccttgaaaa tgagtatttt ttccagaacc ggatatgttt gcgggaagcg gtctcggaat
      721 tcttcctccc gaatgaacgg gaagaaaact gcaaaggaga tataaggaat gtgcaggtct
      781 ttgaattgcg cccgcggttc aggggcctcc ggaaaaatcc tccgtccgaa gtacagcgaa
      841 tcgaaatacg atgcaagttt gcgcgccgtc gccttcgcgc ccttccacgg tggcggtttg
      901 tgtccatact tgcgccggtg attctcccta gcatagttta ttgcgacgag cgaaattgct
      961 ttaaagtcct caatttggaa tcccttcgtc tccaagagaa agaaacccag tgttgggctc
     1021 cacactatcc cgtccacttc gcggatttct ggcgtgtcta agttgaacag ggcgatgccc
     1081 gcctcgtcct tgaggtcagt tttgatccgg tttgccagtt cttgttcagc taagtccgac
     1141 gggataggtg atattatgtc caaaacgtaa cctcggggcc tcattatcag gatatctgtc
     1201 gcaatcgctt tcaagacttt tttgcccgca gccgaatagt tcacgggtgt gcgatcattg
     1261 tgcgcggcac cggaatgggc gcgtccgttt gagaatcgga gtgggttccg tccgcctgtg
     1321 gctacacccg gactctcccc aggttccaaa acacggcatt gtggaatgtg caggaaacct
     1381 cagccgaccg ttacttcgcg tttgccgtca ccaatgacaa gacgagcccc cagcggcttc
     1441 aagaggcgag tgaatacttc gatgcccggg ttcttctttt tcccatcgcg caattcctgg
     1501 atcatggact tggccacgcg ggattttgta gccagcgacc gcgtggctcc ggttgatatc
     1561 tgcaggccgc tgcagttacc gttactttgc cgaaattacg acctgagaga agttcaggaa
     1621 attattcgtc gggccatttg tacccttctt gcatggccta gtctaaacgc cgcctatcgt
     1681 tgcgttagac cgtgagcggt cagtctttct tccatggcac tacgaacatg atgcaccaga
     1741 atgctgtcct gacctggaac atcctgaaat gcgttgtaca tcacaggttc gcagaatatg
     1801 attttgactt ttttatacac gacgcgatga ggaatcttac acacctcttc cggcaaaccg
     1861 aacagagaac gcacgtagcc aggcactgga attgtttcca ggtcctcttc aataggaatg
     1921 atcacaaaag gaatgatagg cgcttttccg cgtgccgcga acgtagcaaa gccttgatga
     1981 aaatctacca tgggagatga aaactgattc ttcaccatgt aatcgtggcc ttccggaaat
     2041 atacccagga tctctcccga ttttaaaacg ctcatggtct tcttgatgct ggcaacagat
     2101 acattgccgt ctatctgcat gggaatgact ccggtttctc tcaccagatt cttgaacact
     2161 ggaattctct gagtgtactc agctgcgatc caggaaatga atcgtggcac aacagacgcg
     2221 acgatgaaag gatccatatc ggaacgatgg ttgccgacaa gaatgcagcc gccttcccgg
     2281 ggcacatact cgagcccttc atgctggatg tctaccatcc agtccagaaa tggctttgca
     2341 atcgccttga ttttgtgaaa ggattccttt tcttgctcaa acacaccttg attctgaaac
     2401 gccggacatt catgtcaatc atgtatccag gacttctcct ggctttcgta tctaccttcg
     2461 ctcgggtcaa ggatcactac ggccgcgtgt tcgtcctggg tcgcaacgca gaagtaacca
     2521 tggatgtgaa gcacggaggc tggaaaccag ttctgcctgt ggtagaagat gcgatccttc
     2581 atctcgcaga agcggatgca cttcgccgcg aaggaaatct agaagaggca gtctgtcttt
     2641 tgcgtgcgat cgaagtgatg ggcacaggtc cttctaaaga cgcaacgcga cggctggaaa
     2701 acattgaccg ctcccgcgca gtcaaagatc tactcgcgca gtgcgatcca atcgtatatt
     2761 tccgcaaaca aacagggaga accacggttc aaagcgatac cgccggactc aaagtatctt
     2821 tcctcggcga atttagaatc atgaaggcaa aatctgtcga ttcgcccact ctcactcaaa
     2881 agatcattta cctggggcat gatgcagaat ccattctcat cgcatacgat ctgtggccgc
     2941 ggtccgtctc tgtagaagac tatcgccgaa tctgggacaa aagacgagga atcacttcca
     3001 caagcacgct gagcgatctg aacgcaggaa tctgctggcc gggaagaatc gaggcaggtg
     3061 gaccaactac cgctcagtgt gcgatttacg aaatggttga aggcaaacag ggcattgaat
     3121 actacgaaac tggcagactg aaaggcatct ttcttgcgtt ttccggcaag gatcgcgcac
     3181 gggatgcact gcgagaactt agaattacaa aatgagaact tctcttcgca tccttcgctt
     3241 tctcagacgt tattcgaccg cgttccttca tccggatcat ccttttctgc gtgactatca
     3301 ggaaagaaga cgcctgcaga tcatcatggt caccggagga ttgcttggcg tctttatgct
     3361 catccgcggc atcctcgtca tccgggacac ccccgagata ggcggcattg tccttacgct
     3421 tgcgtttttg attttcgctg gaatgagcct catcgttgca ttgcgcgcga agggatacag
     3481 atggatcgcc cttgcgcttg tgaccgttgc aggcggagcc aactacgcga attcatatct
     3541 ttctagagta tttcctccgg cggggcttcc attcgcggcc gcagtggtcg tggctttgca
     3601 ctttctattc aaccgctgga tgagccttct gggactggtc gttcagattg tagcgttctt
     3661 accatttctc taccagcagg atttccacta cgcaagcgaa ggcgaacgcc agttctggca
     3721 cacacgattc ttcgcaaact cggcggtctc tctgggcatc atttggattt tgtgcgaagc
     3781 atacagatcc gttcgtgatc aggcagaaat caggctcaat cgcctcaaag cggaacttgc
     3841 aagagacatc actctggcag gaaagatcca gaaggatctt cttccgccta acaagagcga
     3901 aggtggtcca taccagtggc atggatttat gcgtaccatg tctggagcag gtgcgggcgg
     3961 agactacata gatgcacttg aaatccgggg gcacacctgg tttgcgatgg gagacgtaac
     4021 cggacacggc attcaggcag ggcttctggt gatgcttgcg cgaacgtctc ttcatcaggt
     4081 gctgcgagac cagcaagtgc aggatcctgg aaaagtcctt tctcttctgg actattcatt
     4141 ctcacgcact gcaagcagcc tgactgaaaa aagtttcatg acgtttgtgc tcatgcgatt
     4201 tgatgcaaac ggcaaagtaa catttgctgg ttctcatctg aagattctgg tgtatcgtcc
     4261 agcggaagat agaatggata ttctggaaac aaagggaccc tggatcggag tccttacagc
     4321 ggatagacgc gtttcatttg atgaattcac attccagcta gggataggcg acattgttct
     4381 gctttataca gacggaatta cagaagccag ggacgcgctg ggagaagcca tcgaagtcaa
     4441 aggtttgctc aacatgttcc gagaagcgct gcaggaatcg caggatattg aaaaaatcaa
     4501 ccacagaatc attgatctcc tggttcatgc gaccgggaaa aacatattcg atgacgatct
     4561 gtctttattg acgatccgac gcgcccattg aatttcaacc ctatgacacg caaagaacgc
     4621 gacacaatgg gcgaaatcga agtagcgtcg gaccgctact ggggagccca gactcaaaga
     4681 tcactgcatc acttcaaaat tggacacgat cattttccgc gcgagttcat ccgcgccatg
     4741 ggtatcctga aaaaggccgc ggccattgca aatcgcgaac tcggactgct ctcgcaggag
     4801 aaagtaaatc tcatcatgcg cgccgcagat gaagtcatcg atggaaagct cgatgatcac
     4861 ttccctcttg taatctggca aactggttct ggcacacaga ccaacatgaa cgcgaatgaa
     4921 gtgatctcca atcgcgcgat cgaacttgca ggcgggaaaa tgggttccaa agatccaatc
     4981 caccccaacg acgatgtaaa caaagctcaa agttccaacg atactttccc aacaggaatg
     5041 cacattgcgg cggcggagcg aatcgcacag cat
//
LOCUS       DAORFF010000254         5063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9287_length_5063_cov_3.383786, whole genome shotgun sequence.
ACCESSION   DAORFF010000254 DAORFF010000000
VERSION     DAORFF010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9287_length_5063_cov_3.383786"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PKK76_10930"
     CDS             <1..938
                     /locus_tag="PKK76_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HNL69032.1"
                     /translation="IRACFAERGLDPDSFLLAHSYERVMPGNQYLSSITNFWRVFAGY
                     NEAAARKCEEFLLTVVNAEDFPMSRMDTITASETAKVLENSYRATNIAFIEEWGRFAE
                     SVGVDLFQVLDAIRMRPTHNNIRQPGFGVGGYCLTKDPLFARFSATTIFKRPELDFPF
                     SRRAVAVNNQMPLVSVGRLTGMFGGDLRGRKVLLLGVSYRPDVGDTRYSASEIFARAL
                     VEAGASVVYHDPLVHDWPEMNAVVLQKLPPASGFDAIVLAVAHREYRDFDFASWIGEN
                     RPKILDTNNVLSRAQIESLVARQHEVKSIGRGSPS"
     gene            935..1918
                     /locus_tag="PKK76_10935"
     CDS             935..1918
                     /locus_tag="PKK76_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004503054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNL69033.1"
                     /translation="MSRILITGGAGFIGGYLAEACAASGHRVTIADNFARGVRDPFLA
                     ELEKRGVEITQMDLLEQEWALPDDFEIVVHFAAIIGVRHVLERPYSVLRDNVALTDRA
                     ISFGRRQKKLTRFLFASTSEVMAGSLIHLNMPLPTPEDFPLALTDLAQPRTSYMLSKI
                     YGEAMCQHSGIPFTIIRPHNIYGPRMGLSHVVPELLKKSYEKRDGEDLAIASADHSRA
                     MCYISDAVQLIQRLLFADAASGGTFNIGNSETEVTIGDLARIIVSATGRKLNLIAEPA
                     VAGSPSRRCPDMSKTIRVTGYTPSVGVEQGVRLTFDWYKQNVFAASGVSAL"
     gene            1915..3054
                     /locus_tag="PKK76_10940"
     CDS             1915..3054
                     /locus_tag="PKK76_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNL69034.1"
                     /translation="MIPLAIPDLTGNEAKYLQECVTSTFVSSVGAFVNRLEEMVAALS
                     GAAYAVATSAGTTGLHAALIAAGVERDDLVILPSFTFIGSANAIAHCGAMPYLLDVNK
                     DTWTLDPESLAKVLSGTDKKNGKVYHRQTGRRIAAVMPVHVLGIAADMDGIVRAAKEH
                     NLPVVADGAAALGTIYRGRKIADTGADLTVFSFNGNKTVTAGGGGAICGNDASLVQLA
                     RHLTTTARVGADYDHDRIGFNYRMTNLQAAVGCAQLERLSEFVEKKRRIQATYANALT
                     GLSGVLPFPDPAYGKSACWFSGFTLRNGKMESGELRKSLRERGVDARPFWKPIHLQVP
                     FGGCPREELGVTDELWSTLVTLPCSTSLSNADQSTVIKHVQELLG"
     gene            3054..4226
                     /gene="neuC"
                     /locus_tag="PKK76_10945"
     CDS             3054..4226
                     /gene="neuC"
                     /locus_tag="PKK76_10945"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020784366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNL69035.1"
                     /translation="MKTRVCVVTGTRAEYGLLRWVMEGIRLSTHLELQIIATGSHLSP
                     EFGFTYREIESDGFSIDRKIEMVLSADTPSGVAKSMGVGLIGFASAFQELKPDCIVLL
                     GDRYELLSAASAALVARIPIVHIHGGETTEGAFDEAIRHSITKMSHLHFVAADAYRRR
                     VIQLGEDPQNVHVVGGLGLDGISKIALLGRSELEQALDFKLGARNLLITFHPVTLESE
                     SSETQMRALLSALDSFPDIHCIFTMPNADNDSRVIFRLIEEFTARRANSKAFTSLGQL
                     RYLSCLRHVDGVVGNSSSGLIEVPSFRKATINVGDRQKGRLRAQSVLDCNADSATIRS
                     CIERIYSEEFQNLLSQTVNPYGSAGASEKIVKILEARRENRSVKKTFYDLKPGDLA"
     gene            4223..>5063
                     /locus_tag="PKK76_10950"
     CDS             4223..>5063
                     /locus_tag="PKK76_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNL69036.1"
                     /translation="MSKCFIIAEAGVNHNGSLEVARKMIEVAASAGADAVKFQTFRTE
                     ALVSASAPRAEYQIRAMKGDESQAEMLKKLELDESAHSDLIAHAKQVGIEFLSTPFDS
                     LSLRLLTERFGIRTIKIPSGEITNGPFLLQIARSAEKIILSTGMSDLSEVEAALAIIA
                     FGFLNPTGNPDRSSLIEIGDSQKAWDELRNRVILLHATTEYPAPMSEVNLRAMDTLAS
                     SFGLKTGYSDHTRGIHVAVAAVARGACVIEKHFTMDRGMDGPDHKASLEPAELREMVH
                     AIRD"
BASE COUNT         1147 a         1210 c         1384 g         1322 t
ORIGIN      
        1 agattcgggc ttgttttgca gagcgcggat tggatccaga cagctttcta cttgctcatt
       61 catacgagag agtcatgccc ggaaatcagt acctatcctc gattacgaat ttctggcgtg
      121 tctttgcagg atacaacgaa gctgctgctc gcaaatgcga agagtttttg ctaacagtgg
      181 tcaatgcaga ggattttccg atgtcgcgca tggacacgat tacagcgtcg gagactgcca
      241 aggttctaga aaatagttat cgagctacga acattgcatt catcgaagag tggggtcgct
      301 ttgctgaaag tgttggcgtt gatttgtttc aggtgcttga tgccatccgc atgcggccaa
      361 cgcataacaa tattcgtcag cctggattcg gtgtcggtgg gtattgtttg accaaggatc
      421 cattgttcgc gcggttttct gcgacaacaa ttttcaagcg ccctgaactg gatttccctt
      481 tctcacgtcg ggctgttgcg gtgaacaatc aaatgcctct cgtcagtgtc gggcgcctaa
      541 caggtatgtt cggcggagat ttgcgaggga gaaaggttct cttgctgggt gtaagttatc
      601 ggccagatgt aggagatact cgttattcag cttccgaaat cttcgcacgc gctcttgttg
      661 aggctggcgc gtcagttgtt tatcacgatc ctctggtcca tgactggccg gagatgaatg
      721 cagtcgttct tcaaaagcta cccccggcat caggttttga cgcaattgtt cttgctgtag
      781 cccatcgcga gtatcgggat tttgattttg cttcctggat tggagagaat cggcctaaga
      841 ttctggatac aaacaatgtg ctatcaagag ctcaaatcga gtccctggtt gcccggcagc
      901 atgaagttaa gtctattgga agaggatcac catcgtgagt cgcattctta tcaccggcgg
      961 tgccggtttc attggcggat atctggcgga ggcttgcgca gcatccgggc acagagtgac
     1021 gatcgctgac aacttcgcac gtggcgttcg tgatcctttt ctcgcagaac ttgagaaacg
     1081 tggcgttgaa atcacgcaaa tggacttgct agagcaagag tgggcactgc cggatgattt
     1141 tgaaatcgta gtgcatttcg cagcaatcat cggggtaagg catgtgcttg agcgaccata
     1201 ttctgtgctg cgcgacaacg tagctctaac cgaccgcgcg atttcgtttg gtcggcgtca
     1261 gaaaaaactg accaggtttt tgtttgcgtc cacctccgag gtgatggcgg gatcgttgat
     1321 acatcttaac atgccgctgc cgactcctga ggattttccc cttgctctga ctgatcttgc
     1381 tcagccgcgc acttcttaca tgctttctaa gatttacggg gaagccatgt gtcagcattc
     1441 gggcattcct tttacgatca ttcgacccca caatatctat ggccctcgga tggggctttc
     1501 tcatgtggtt ccggaattac ttaagaaatc ttatgaaaaa agagacggag aagatcttgc
     1561 catcgcttcc gcggatcatt cgcgtgcgat gtgttacatc agcgatgcag tgcaattaat
     1621 tcaaaggttg ctctttgccg atgctgcttc cggcggaact ttcaatatcg gaaactctga
     1681 aacggaagtt acgataggcg atcttgccag gatcatcgtt tctgcaacgg gcagaaagct
     1741 gaatctgatt gctgaacctg ccgttgccgg atctccttcg cgtcgttgtc cagatatgag
     1801 taagaccata cgggttacgg gctatacacc ttcggttggc gtagagcagg gagttcgttt
     1861 gacattcgat tggtataagc agaatgtatt tgctgcatcc ggagttagcg cactatgatt
     1921 cctctggcaa ttccagatct cactggaaac gaggcaaaat acctccagga atgcgtcaca
     1981 agtacgtttg tgtcgtcagt gggtgctttt gtgaatcgtc tcgaggagat ggtagccgct
     2041 ctgtccgggg ctgcgtacgc ggtcgccacc tctgccggga cgacagggct gcatgctgct
     2101 ctcattgcag cgggcgtgga gcgtgacgat ctggtgattc ttccgtcatt tacttttatc
     2161 gggagcgcaa atgcgattgc acattgcggc gccatgccat atctactgga tgttaacaaa
     2221 gacacctgga cattggatcc cgaatctctg gcaaaggtgc tttcaggtac cgacaagaaa
     2281 aacggcaagg tttatcacag acaaactggg cggcgcatag cagcggttat gccggtgcac
     2341 gttctgggta tcgcggcaga catggatggg atcgttcgcg ccgcaaaaga gcataacctt
     2401 cctgtggttg cggacggagc ggcggccctc ggcactattt atcgcggcag gaagatcgcc
     2461 gataccggcg cggatctgac tgtgttttcg tttaacggaa ataagacggt cactgcaggt
     2521 ggaggcggag ccatatgcgg caacgatgca tccctggttc aactcgctcg tcatctgacg
     2581 acaacagctc gcgtcggtgc agattatgat cacgatagaa ttggattcaa ttatcgcatg
     2641 acgaatcttc aggcggccgt tgggtgtgcg cagcttgaga ggctgtctga gttcgtggaa
     2701 aaaaagcgtc gcattcaggc aacgtacgcg aatgctctga ctggcctgtc tggagtcctt
     2761 ccttttcctg atccggccta tggcaaatct gcttgctggt tttccggatt cacacttcgc
     2821 aacgggaaaa tggagtcagg agagttgagg aagagtctgc gtgagagggg agtggacgcg
     2881 cgtcctttct ggaagccaat tcatcttcag gttccgtttg gcggttgtcc cagagaagag
     2941 ctgggtgtca cagatgaact gtggtctact cttgttactc ttccatgttc gacttctctc
     3001 agtaatgcgg atcagtccac ggtaatcaag cacgtccagg aacttctagg gtgatgaaaa
     3061 ccagggtctg tgttgttacg ggtacgcgcg ctgagtatgg acttcttcgc tgggtcatgg
     3121 aagggattcg tctttccact caccttgaat tgcagatcat cgcaaccggg tcgcatctat
     3181 cccctgagtt tggatttact taccgcgaga ttgaaagcga tggcttttcc atagatagaa
     3241 aaattgaaat ggtgctcagt gcagatacgc cctctggtgt tgctaaatcc atgggcgtgg
     3301 ggctgatcgg atttgcatcc gcatttcagg agttgaagcc tgactgcatt gtactgctcg
     3361 gtgatcgcta tgaacttctg tcggccgctt cagctgccct tgtggcacga attccaatcg
     3421 ttcatattca cggcggagaa acaaccgagg gtgcgtttga tgaagccatt cgccactcta
     3481 ttacgaagat gtcacatctt cactttgttg cagcggatgc ctaccgcagg cgcgtgattc
     3541 agcttggaga agatccacaa aacgtgcatg tagtcggggg actgggtctc gacggaattt
     3601 ctaagattgc tttgctcggg cggtcggaat tggagcaggc gcttgatttt aaactcggtg
     3661 cgcgaaatct tctgatcacg tttcatccgg ttacgctgga gtctgagtcc tctgagactc
     3721 agatgcgagc gttgctcagc gctctggaca gttttccgga catacattgc attttcacga
     3781 tgcccaatgc agacaatgac agccgcgtta tctttcggtt gattgaagaa ttcacggcgc
     3841 gtcgcgcaaa ctcaaaggcg ttcacatccc tcgggcagct gaggtacctt tcgtgtttac
     3901 ggcatgtgga cggtgttgtg gggaattcct ccagcggttt gattgaagtt ccttcctttc
     3961 gcaaagctac aatcaatgtg ggcgatcgtc aaaaaggtcg ccttagagcg caaagtgtcc
     4021 tggattgcaa tgcagactcc gcaacaattc gttcttgcat tgaaaggatc tattcggaag
     4081 aatttcagaa tctgctttcg caaaccgtaa acccgtatgg cagcgcaggt gcgagtgaga
     4141 agatcgtgaa gattctggaa gccagacgcg aaaatcgatc agttaagaag acattctacg
     4201 atctcaagcc cggagatctc gcatgagcaa gtgcttcatt atcgctgaag ctggcgtcaa
     4261 tcacaacgga tctctcgaag tcgcgcgaaa gatgatcgaa gtcgcagcca gtgcgggtgc
     4321 ggatgcagta aagtttcaga cttttcgcac agaagcgctg gtcagcgcat ctgcgccgcg
     4381 tgctgagtat cagattcgcg ccatgaaagg agacgaaagt caggcggaga tgcttaagaa
     4441 actggagctc gatgaatccg ctcacagcga tctgattgcg cacgcaaagc aagttgggat
     4501 cgaatttctg tctacacctt ttgattctct gagtcttcgt ctcctcacgg aacgcttcgg
     4561 aattcgaacc atcaagattc cttctggaga gatcacgaac ggcccctttc ttcttcaaat
     4621 tgctcgttcc gctgagaaaa tcatcttatc gaccggcatg agcgatcttt cagaggtgga
     4681 agcggctctg gcgatcatag catttggttt ccttaatccg acaggaaatc ctgacaggtc
     4741 ttctttgatc gagatcgggg attctcaaaa agcgtgggac gaactgcgaa acagggtcat
     4801 ccttctgcac gcaaccacag agtatcccgc ccccatgagc gaagtgaatc ttcgtgcgat
     4861 ggatacactc gcttcttcct tcggtttgaa aacaggctac tctgaccata cccgtggcat
     4921 tcacgtcgcg gtggctgccg ttgcacgagg cgcgtgcgtt atagagaagc attttacaat
     4981 ggatcgcggt atggatggcc ccgatcacaa ggcgtctctc gaacccgcgg aactcaggga
     5041 aatggttcat gcgattcgtg acg
//
LOCUS       DAORFF010000255         5061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9293_length_5061_cov_4.276868, whole genome shotgun sequence.
ACCESSION   DAORFF010000255 DAORFF010000000
VERSION     DAORFF010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9293_length_5061_cov_4.276868"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1137)
                     /gene="meaB"
                     /locus_tag="PKK76_10955"
     CDS             complement(151..1137)
                     /gene="meaB"
                     /locus_tag="PKK76_10955"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNL69037.1"
                     /translation="MDIDTNALFAGDRRMLARAITLIESTRPEDEMAAEELLHRVLPR
                     IGSSVRIGISGSPGAGKSTFIESFGMKCIEAGSKVAVLAVDPSSSITGGSILGDKTRM
                     SKLGSHESAFIRPSPSGGSLGGVNRRTREVIWLVEAAGFDRILLETVGVGQSEIDAAN
                     MTDFYLLIQLPNAGDELQGIKKGILELADCVFVNKADIDKTLAEKARGELSSALHYQR
                     ARTSGWQIPVLVGSAATGAGLVELMQVIEQFCAHTGRDTGPGSAFHSRRSAQLKEWMH
                     DRIKDKMIRSFYGHPDVQKALPEVEKQVASGALTAQEAMQKLFDVYRLPNAH"
     gene            1232..2206
                     /gene="rfaD"
                     /locus_tag="PKK76_10960"
     CDS             1232..2206
                     /gene="rfaD"
                     /locus_tag="PKK76_10960"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNL69038.1"
                     /translation="MILITGAAGLIGSAVARELNRRGRRDLLLVDHLGTSEKWMNLRS
                     IQYASYIEKDQLLTALQTGSHSLFGINLIFHLGACSSTTERDASYLINNNYQYSIRLA
                     DFAAQKGIRMVYASSAATYGEGENGFEDDLTRVDRLRPLNPYGYSKHMFDLWLRDRRF
                     EPSFAGIKYFNVYGPNEYHKGEMMSVVLKAYRQILADGKIKLFKSYRSDYKDGEQKRD
                     FLYVEDAARMTVYLGLESQALGLFNAGSGGANTWVDLANAIFQAMNRQPQIEFVEMPE
                     HLRAKYQYYTCAPMDTLRSAGYSAPVTPLADAVKDYVQNYLMKDESRA"
     gene            2190..>5061
                     /locus_tag="PKK76_10965"
     CDS             2190..>5061
                     /locus_tag="PKK76_10965"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNL69039.1"
                     /translation="MNRAPESRFFFATDLSTLARKLTDQIAKDKKRDPLQPRRIIVPN
                     RNVARWLEMRLADWSGVCGNVDFQFLEPGIGNRIEEITGKKRALASREDLTLGILECF
                     FEGQVSGALARYIESRNSTDLYHLAAKLASLFQDYEYHRAEWADRWLKGDFAAPAGAH
                     DQAELYFNVMRRMNARGLSNLRGFASFIQEGSAQSEPLILFGVSQISKLHVDILFQLS
                     QLLPLEYYMLDFLQPQKQKWYPCNGLFDEPVGPVRSWLSVQSQQIKILLDQKKENHVW
                     QASLSVPEEASILSTLQRVFTGAPIKKGKEAHSANAGRSPDSSVVIVGCPGVVREAET
                     ILFDIVDRMKEDPTLQLTDIAILVPDMKKYRPALEAVFERDVDAQGRPGIPFSITDFR
                     SGDASLYAAGAHALLDLMDGEFRRSQVFALLRNPCFQHARGISEKTVADWSDYIDRLA
                     VYHGMDPEDPYSFMQGLRRLRLGYVMNLEEGDFAGIVPFHDIFIDEREAGLLSRTIHD
                     LALEIEALRAKAAFEMPEALRKFLEAHLAVPPSRKHERRVQSSVMGMLVRLSHTKVQL
                     PVEIMREIILASASGLSANRGDYLSEGVTISSLQPMRPIPFRITYIAGMEEGSFPGRA
                     DESAINLRNAHPAAGDVSLPEANRMLFLESFLAARDRVFITYNARDTQKDAHFAPCSV
                     VQELIEAAAAIGIQLVPRMVPIHAAVVREGFIPEPESVTALLESVSAGKSAAPDQIEI
                     VKEYLSSRNVSVKHRKNASRRIVSVTRLKAYLEDPVASVLRDAAGMREELEVDSTLDD
                     VEPFYMRSFQYPMMWREAIQAYFQANGQIPVNQIVENLYGNLERRLRVPAGAFGKVQK
                     MQILARLDPSKWQSFTEKKFAPASLSDVSATSGDVSIVGEIPFLRETPGLIEILAPAS
                     NSRARTLLPSYVFFLAWLSARRIDASLTVHVVTVSDGKNPASK"
BASE COUNT         1185 a         1237 c         1352 g         1287 t
ORIGIN      
        1 tctcgaaaga gcctaccttc tgtcgtactg gagatgcctc cacgatcaac cttccggccg
       61 gcgagcgaga gcacgcagaa gccagaatcg caaatgaaaa gagcacaaaa agatggatgg
      121 accgtaattg catgaagccc ccgaaagcgc ctaatgagcg ttcggcaagc ggtagacgtc
      181 aaatagtttt tgcattgctt cttgagctgt aagtgcaccc gaagcgactt gtttctctac
      241 ttccggcagc gccttttgaa catcaggatg accgtagaag ctgcggatca ttttgtcttt
      301 gatgcgatcg tgcatccatt ctttcagttg agccgatctt ctagaatgaa atgcacttcc
      361 tggtccggtg tctctcccag tatgggcgca gaattgctca atcacctgca tcagctccac
      421 cagtcccgcg cctgtggcag cgcttcccac aagcactgga atttgccagc ccgatgtgcg
      481 cgcacgctgg taatggagcg cagaggagag ttctccacgc gctttttctg caagtgtttt
      541 gtctatgtct gccttgttca cgaaaacgca atcggcgagt tccagaattc ctttcttgat
      601 tccctgcagt tcatctcctg cgtttggcag ctggatgagc agatagaagt ccgtcatatt
      661 ggccgcgtca atttccgatt gtcctactcc gactgtttcg agtagaatcc tgtcaaaccc
      721 tgccgcttcc acaagccaga tcacttcgcg tgtccggcga ttcactccgc ccagggatcc
      781 acccgatggg ctgggtcgaa tgaaggcgct ctcatggctg ccgagttttg acattcgtgt
      841 tttgtctcca agtatggatc cacctgtgat gcttgaactt ggatctacgg cgagcacagc
      901 gactttgctt cctgcctcaa tgcacttcat tccgaacgat tcgatgaatg tagattttcc
      961 ggctcctgga gagccgctga ttccaatgcg aacagaggaa ccgattcgcg gtagcacgcg
     1021 gtgcagaagc tcttcggctg ccatttcatc ttctggtcgc gtgctttcta ttagcgtgat
     1081 ggcgcgggcc agcattcgcc gatcacctgc gaagagtgca ttcgtgtcga tgtccattgc
     1141 cagcccgctt caggctcggg gattgccttt gccaggcaac cgaaaaacct tttgaggttg
     1201 actctgtcga cgcccgcaaa ttcaatctgg catgattctg attacgggag ctgcgggcct
     1261 gattgggagt gccgtggccc gcgaactaaa ccgtcgtggt agaagagacc tcctcctcgt
     1321 ggatcatctg ggcacttcgg aaaaatggat gaatctgaga tccattcaat atgcgagcta
     1381 cattgagaaa gaccagcttc tcaccgcgct tcaaacgggg tcacatagtt tgtttggaat
     1441 caatctgatc ttccacctgg gtgcctgcag ttccaccacg gagcgcgatg ccagttattt
     1501 aataaacaac aactatcaat attctataag acttgcggat ttcgcggccc agaaaggcat
     1561 tcggatggtc tatgcgtcga gtgcagccac atatggggaa ggggaaaatg gattcgagga
     1621 tgacctgacg cgcgtagatc gtcttcgccc tttgaacccg tatggctact ccaagcatat
     1681 gttcgacctg tggttgcggg atcgtcgttt tgagccttct tttgcgggca tcaagtactt
     1741 caatgtatac ggacccaatg aataccacaa aggagagatg atgtctgtgg tgctgaaagc
     1801 ttacagacag attctggcgg acggcaagat caagctcttc aagagctata gatcggacta
     1861 caaggatggg gagcaaaagc gtgatttcct ctacgtggag gatgcggcca ggatgactgt
     1921 ttatctgggg cttgaatctc aggcactggg attgttcaac gctggaagtg gtggcgcgaa
     1981 cacctgggtg gacctcgcca atgctatctt ccaagcgatg aacaggcagc cacaaataga
     2041 gtttgttgaa atgccggagc acctcagagc aaagtatcag tactatacct gcgcgcccat
     2101 ggacactctt cgatccgccg gttatagtgc tccagtgact cctcttgcgg atgcagtgaa
     2161 ggactatgtt cagaattatt tgatgaagga tgaatcgcgc gcctgaatcg agattcttct
     2221 tcgccacaga tctaagtacg cttgcgcgca aacttacaga tcaaatcgcg aaggataaga
     2281 agcgcgatcc attgcagccc aggcgcatca tcgttccaaa caggaacgtc gcgcgctggc
     2341 tggagatgcg ccttgcagac tggagtggtg tctgtggaaa tgtcgatttc cagtttctcg
     2401 aaccaggcat tggcaataga atcgaagaaa tcacagggaa gaagagggct ctagccagta
     2461 gagaagatct gacccttgga attctggaat gcttttttga aggtcaggtt tccggtgccc
     2521 tggccaggta catagaatcc agaaatagca cggatcttta tcatctagca gcaaaactgg
     2581 cgtctctctt tcaggactat gaataccaca gagcggaatg ggccgatcgg tggctgaagg
     2641 gggattttgc agctccggcc ggtgcacatg atcaggcaga attgtatttt aacgttatgc
     2701 ggcgaatgaa tgcaaggggt ttgtccaatt tgcgaggatt cgcatccttc attcaggaag
     2761 gaagcgcgca atctgagcct ttgattcttt ttggagtttc tcaaatttca aagctgcatg
     2821 tggacattct ttttcagctg agccagcttt tacctcttga atattacatg ctcgacttcc
     2881 ttcagcctca aaaacaaaaa tggtatccgt gtaacgggtt gtttgatgag ccggttgggc
     2941 cggttcgttc ctggctctct gttcagtctc agcagatcaa gattctgctg gaccaaaaaa
     3001 aggaaaacca tgtctggcaa gcgagtcttt cagtgccaga agaagcaagt attctttcca
     3061 ctctgcaaag agtgttcaca ggggctccca ttaagaaggg gaaggaagct cattcagcga
     3121 atgcaggtcg ttctccagat tcgagtgtcg tgatcgttgg ctgtccggga gttgtgcgcg
     3181 aagcggaaac cattctcttt gatatcgtgg acagaatgaa agaagatcca acgttacagc
     3241 tgacggatat agcgattctg gttcctgaca tgaagaagta tcgtcctgcg ctcgaagccg
     3301 tgttcgagcg tgacgtcgac gcgcagggtc gccctggaat tccttttagc atcaccgact
     3361 ttcgttctgg agatgccagc ctgtacgctg ccggtgcgca tgccctactc gatctaatgg
     3421 atggagagtt tcgacgcagc caggtatttg ctcttcttcg caatccttgc ttccagcatg
     3481 cgcgcggtat cagtgagaag actgtggcag actggtccga ctacattgat cgcctcgcag
     3541 tgtatcatgg catggatccg gaggatcctt acagtttcat gcaaggtctt cgcaggcttc
     3601 gtctcgggta cgtgatgaat ctggaggaag gcgactttgc agggattgtt ccgtttcatg
     3661 atatcttcat agatgagcgc gaagcgggac tgttatcgcg gacgattcat gatctggcac
     3721 ttgagattga agcgcttcgc gcgaaagccg cgtttgagat gccggaagct ctgcgaaaat
     3781 ttttggaagc gcaccttgct gttcctccat ctagaaagca tgagagaaga gtgcagagtt
     3841 ctgttatggg aatgctggtt cgtctctcgc acacgaaagt gcagctcccc gttgaaatca
     3901 tgcgagagat cattcttgcc tctgcgtctg gtctgagcgc gaatcgcggc gactatctgt
     3961 cagaaggtgt gaccatctct tcgctacaac ccatgcgacc gattccgttt cggattactt
     4021 atattgcggg aatggaagag ggcagttttc cgggtagagc ggatgagtct gcgatcaact
     4081 tacgaaatgc gcatcctgca gccggtgatg tttctcttcc ggaagcaaac aggatgctgt
     4141 ttctggagtc gtttctggca gccagagatc gagtttttat tacttacaat gctcgggata
     4201 cgcaaaagga cgcgcacttt gctccttgtt ccgtggtcca ggaattgata gaagccgctg
     4261 ctgcgatcgg gattcaactg gttcctcgga tggtgccgat tcatgcggcc gttgttcgtg
     4321 aagggttcat tcccgagcct gagagtgtca ccgcacttct ggaaagcgtt tctgcgggca
     4381 agtccgccgc gccagatcag attgagattg ttaaagaata tttatcatcc aggaatgtat
     4441 ccgtaaaaca cagaaagaac gcatcccgac gaattgtaag cgtcaccaga ttgaaggcat
     4501 atctggagga tcctgtggca tctgttctca gagacgcagc cggtatgcgg gaagaactcg
     4561 aggttgattc aacgttagac gacgtagagc ctttctatat gagaagcttt cagtacccga
     4621 tgatgtggag ggaagcaatc caggcttact ttcaggcaaa cggtcagatc ccggtgaatc
     4681 aaatcgttga aaatctctac gggaatctgg agagacgtct gcgggttccg gcaggggctt
     4741 ttggaaaagt tcagaaaatg cagatcctgg ccaggctcga tccatccaaa tggcagagtt
     4801 tcacagaaaa gaagtttgcg cctgcttccc tttcagacgt atctgcgacc agtggcgacg
     4861 tttccattgt aggcgagatt ccatttctgc gcgaaactcc cggattaatt gaaattctag
     4921 cgcccgcgag taattcgaga gcccgcacac tcttgccatc gtacgtattc tttctggcat
     4981 ggctttccgc gcgcaggatc gacgcatccc tgactgttca cgtggtgact gtctcggatg
     5041 gtaagaaccc tgcttccaaa a
//
LOCUS       DAORFF010000256         5043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9339_length_5043_cov_3.192462, whole genome shotgun sequence.
ACCESSION   DAORFF010000256 DAORFF010000000
VERSION     DAORFF010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9339_length_5043_cov_3.192462"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..1093)
                     /locus_tag="PKK76_10970"
     CDS             complement(296..1093)
                     /locus_tag="PKK76_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNL69040.1"
                     /translation="MRNSDNMSIAPDIVNTVQNRLTSIEVEHNVEILFAIESGSRAWG
                     FASEDSDYDVRFIYRHPIPWYLNILPKRDVIEYPIVDIMDYSGWDLRKAFFLANKSNP
                     VLFEWLQSPIVYRKNDRFFDSFFPAAMAYFSPISTVYHYLHMATNNYREYLKRDYVKV
                     KKYFYVLRPIFACQWVHRFSSAPPMDFEELKNAVLENQELNGVISDLLIRKRSGGELG
                     EEPKIELINDFVEHALFSLHTTAKDFDPEKKPDSQLLNALFISIVLK"
     gene            complement(1124..1837)
                     /locus_tag="PKK76_10975"
     CDS             complement(1124..1837)
                     /locus_tag="PKK76_10975"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNL69041.1"
                     /translation="MIRFSIILSSFLALACQSFVPRPPQPEPRLVAVTGTASVRKAPD
                     IIYVEIMSFAESTDPRAAFESALKTSDTTVAHLRNSYNLATEDVAVGSLSISPAYKTI
                     KGERTDIIEKYKAQLLLHLSIRNFADAPKIISDVLKLGIGSVQSFRYDIDRRSEIVRA
                     AQVLAFEDARAKALALAKAAGFRSVSLITIEIDNSRAHFSLNDSVQGYTARNFESFQM
                     AKETLDVEAQVTCRFEMLP"
     gene            complement(1902..2393)
                     /locus_tag="PKK76_10980"
     CDS             complement(1902..2393)
                     /locus_tag="PKK76_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69042.1"
                     /translation="MKRIFHTVLASAALALAHGIHASDGQEPKKPVCPLIWIGSDEME
                     GNGNFFSARLKLEITGTEGAFHVEAWRCHGARATTPEDCRDLIVHSKALVRGRVSITG
                     QKVRFAGESVTEEIFWPEGMATGKYCLDTLDGTIEGSLFKAVHSDPCNPSTRVILKRV
                     DCT"
     gene            complement(2420..3046)
                     /locus_tag="PKK76_10985"
     CDS             complement(2420..3046)
                     /locus_tag="PKK76_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNL69043.1"
                     /translation="MKDSIHKKPRQNLRERARAIKPFRCKNLIAVLEEPADYKNVGTV
                     IRNVNALGVEKAYVIDSRNLLPGDWAEMRDRKSLSATSASAIKWSFVRTFQSTDECIA
                     HLKKKGFVSMATSPHRKGRENIVLHEGNYTTPAKLAIWFGNESQGISELAVAASVACI
                     NIPMFGLIESLNLGTSTGIVLYEVTKQRRGYQSQYKRAGKRMPIPKKR"
     gene            complement(3058..3915)
                     /locus_tag="PKK76_10990"
     CDS             complement(3058..3915)
                     /locus_tag="PKK76_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69044.1"
                     /translation="MKRFLLLTSLLATAVYCQPPYLSSQEAFAVYATEFYGKKLPGTR
                     VTKLQNFSLSFQLPNKEPAILFLDNPFLACKSAPSDCETTLLKYLPSPDSADLKGDRL
                     SIRPMLKSKEYASQLQSMFNSGPKKQKAPTTPFIGDITILYAFDFPERMAVVDEEDMR
                     AVGASDVESLHRLALLNMEARTGELAYKEIQPESGIYALTYEDSLAPSRVLLQDKWAA
                     VARKIKGNPILAIPSREFVLFVAEGDAARVRSLRELVRKVFSGEDHAVSMQLMHWTPR
                     GWELYSDEQ"
     gene            complement(3948..4589)
                     /locus_tag="PKK76_10995"
     CDS             complement(3948..4589)
                     /locus_tag="PKK76_10995"
                     /inference="COORDINATES: protein motif:HMM:NF039641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6064 family protein"
                     /protein_id="HNL69045.1"
                     /translation="MKIPFTTEQFLGVFAAYNQSVWPAQVALFAVALVILALSLKTFS
                     FSSIMSGSILGVLWIWMGIAYHIVQFTSINPAAYVFGIACILQGLIFLWNGFRRTLEF
                     RFSWDVRGWAGGLLILYALILYPIVGSLLGHVYPSSPTFGLPCPTTIFTFGMLLWVRK
                     PPLIVLIIPVLWSIVGFSAALQLGIKEDVGLPMAAALVIILQVFATKAPQPVR"
     gene            complement(4668..>5043)
                     /locus_tag="PKK76_11000"
     CDS             complement(4668..>5043)
                     /locus_tag="PKK76_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69046.1"
                     /translation="AHQSTAAVSPLRFPPASQSLSENAESVGPEFHAWYSAGHMTQVR
                     SALAPGYIMVVVGHCFAATQDAEAQSCSEQIARVVLNGLVDEGFPRSKLGSRGTGDAP
                     LEGYPPRENFVHFRIEREQDSK"
BASE COUNT         1253 a         1206 c         1303 g         1281 t
ORIGIN      
        1 aagcgcgaat gttgcggtaa gaatcgcaaa aattgaatgg gtccaaccac gatgtccata
       61 aaaagggtcc tgggaagagt aagagccggt gaagctatcg acatccggta gcacggccat
      121 gaaggaaccg gccgccaggg atagaatgta ggaacctcgg aacgctctat tcagggaggc
      181 tcccgcataa acggtatttg ccgccgtcag gccatataaa aagtgttccg ggggagtcat
      241 atacctgtct atcctgtgcg ccttgaattc tgtagattgt attttctcgt ctatttcatt
      301 tcagtacgat tgaaataaaa agcgcattca atagttgaga atctggcttt ttctccggat
      361 caaagtcttt tgctgttgtg tggagagaaa acaaggcatg ttccacaaag tcgttaatta
      421 attcgatttt tggttcttct cccagttccc caccgcttct ttttctgatc agtagatctg
      481 aaatgactcc attcaattcc tgattttcta aaacagcgtt cttgagctct tcaaaatcca
      541 ttggtggcgc tgaactaaat cgatgaaccc actggcaggc aaatatgggg cgaagaacat
      601 agaagtattt ttttactttc acataatccc gcttcaggta ttcgcgatag ttgttcgtcg
      661 ccatgtgcag gtaatgatag actgtggaaa tcgggctgaa gtaggccata gctgcgggaa
      721 agaagctatc aaagaagcga tcattcttgc gatacacgat cggtgattgc agccattcga
      781 aaagcacagg gttagacttg tttgcaagga agaaggcctt acgaagatcc cagccgctgt
      841 agtccatgat gtcgacgatc ggatattcaa tcacgtctct cttcggaagt atgttcagat
      901 accacggaat ggggtggcga taaatgaatc gtacatcgta gtcgctatct tccgacgcaa
      961 atccccatgc acgagaaccg gactcgatgg cgaaaaggat ttcgacgttg tgctcgactt
     1021 caatggaagt cagtcgattt tgcactgtat tcacaatatc aggtgcgatg ctcatgttgt
     1081 ctgaattgcg cataacgtta gtttccggca tcctgaggcc attttacggg agcatttcaa
     1141 atcgacaggt tacctgggct tccacgtcaa gagtttcctt tgccatttga aatgattcaa
     1201 agttgcgtgc tgtgtaacct tgaacagaat cattcaggga gaaatgcgcg cggctattgt
     1261 ctatttcaat tgtgatgagg gaaacgctgc gaaaaccagc cgcttttgcc agagcaaggg
     1321 ctttcgctcg cgcgtcttca aaggcgagaa cttgcgccgc tcggactatc tcagatctgc
     1381 gatcaatgtc atatcgaaat gattgcacgg agccaatccc gagtttcagc acgtccgaaa
     1441 taatctttgg agcatctgcg aaatttcgga tggaaagatg aagtaggagt tgcgctttgt
     1501 atttttcgat gatatccgtc cgctcaccct tgattgtctt gtaagccgga gaaatggaaa
     1561 gggacccgac ggccacatcc tctgtggcca gattgtaact gtttctcaaa tgagccactg
     1621 tcgtgtcaga tgttttgagc gcactctcga atgcggcgcg cggatctgtg ctttctgcga
     1681 aggacatgat ttcgacatag atgatatcag gcgcctttcg gacggacgcg gttccagtca
     1741 ctgcaacaag acgcggttct ggttggggcg gccgcggcac gaatgactgg caggctagcg
     1801 caaggaaact actcaagatg atcgaaaatc ttatcattca ttttctccga ttctgggata
     1861 ttctttgatc ctgtgtcgtt tgattcgcac gccggagcgc actatgtgca atctactctt
     1921 ttcagaatca ctcgagtgga cgggttgcat ggatcggaat gaactgcttt gaaaagactt
     1981 ccttcgatgg ttccgtcgag cgtgtccagg cagtacttgc ccgtggccat tccttcgggc
     2041 cagaagattt cttccgtgac cgattcacct gcaaagcgca ctttctgacc ggttatagaa
     2101 acgcgtccgc gaaccagagc ttttgaatga acaataagat ctctgcagtc ttcgggtgtg
     2161 gtcgccctgg caccgtgaca tctccaggct tccacatgga aggcaccttc tgttccggtt
     2221 atttccagtt tgagtcgagc agaaaagaaa ttgccgttcc cttccatttc atcggaaccg
     2281 atccatatca aagggcaaac aggtttcttt ggttcctggc catcgcttgc gtgaatgccg
     2341 tgagcgagag cgagagcggc tgaagccaga actgtgtgaa agattctttt catgttgtct
     2401 cctgtggatg caatggcgct taacgtttct ttggaatagg catgcgtttc cccgctcttt
     2461 tatactggct ctggtaaccg cggcgctgtt tcgttacttc atacaaaacg attcccgtcg
     2521 aggttccgag attcaggctt tcaattagac caaacattgg aatattgatg cacgcaacac
     2581 ttgccgcgac ggccaactcg ctgatgccct gggattcatt tccaaaccag atcgcaagtt
     2641 ttgccggcgt tgtgtagttt ccctcgtgga gaacaatgtt ctcacggcct ttacgatgtg
     2701 gagatgtagc catagaaacg aagccctttt tcttgagatg cgcgatgcat tcgtctgtac
     2761 tctggaaggt gcggacaaag ctccatttga ttgcggaggc ggaagtggcc gacagcgatt
     2821 ttctgtctcg catttcagcc caatcgccgg gcagcagatt tctggaatca attacatagg
     2881 ccttctcaac gccgagggca ttgacattgc gaatgaccgt acccacattc ttgtaatccg
     2941 caggttcctc aagcactgcg atgagattct tgcagcggaa gggcttgatt gctctggcgc
     3001 gttccctgag attttgccgc ggttttttat ggatggaatc tttcataaat tatgaactca
     3061 ctgttcatct gaatacaatt cccatcccct gggcgtccag tgcatcaatt gcatggaaac
     3121 agcatgatct tcgcctgaaa acaccttacg gactagttcg cgcaaggatc gcacgcgcgc
     3181 tgcgtcgcct tccgctacga acaaaacgaa ttctcgcgaa ggaatggcaa ggatcggatt
     3241 gcctttgatt tttcgcgcaa cggcggccca cttatcttga aggagcactc tggacggagc
     3301 cagtgaatcc tcatacgtaa gtgcgtaaat cccggattca ggctggattt ctttgtaagc
     3361 cagttcgcct gttctggctt ccatgttgag caacgcgagg cgatgcaggg attctacatc
     3421 actcgcgccc actgctcgca tgtcttcctc gtcgactaca gccatgcgtt ccggaaagtc
     3481 aaatgcatag agtatggtga tgtcgccgat aaacggtgta gtcggggctt tttgcttttt
     3541 tgggcccgag ttgaacatgc tttgcagctg ggatgcatat tctttggatt ttagcatggg
     3601 gcggatggac aagcgatcac ccttcaagtc ggctgaatcc ggagagggga gatacttcaa
     3661 gagcgtagtt tcgcaatccg agggtgcact cttacaggcc aggaatggat tgtccagaaa
     3721 gaggatggcc ggttctttat tgggtaactg gaaggagagc gaaaaatttt gcagcttcgt
     3781 tactctggtt ccaggcagtt tctttccgta gaactccgtt gcgtagacgg caaatgcttc
     3841 ctgtgagcta aggtaaggag gctgacagta aactgcagtt gcaagaaggc tcgtcagtaa
     3901 gagaaatcgt ttcatgtgat atcctataga gtcaatgtcg cttaacgtta acggacgggt
     3961 tgtggtgcct tcgttgcaaa aacctggagg atgatcacga gcgctgcggc cattggcaat
     4021 ccaacgtctt ccttaattcc aagttgcagc gctgcagaaa agccgactat agaccagagc
     4081 acgggaatga tcaggacgat caacggaggc tttcgcaccc agaggagcat tccgaaagtg
     4141 aagatggtcg tggggcaggg caaaccaaac gtgggcgagg atggatagac gtgcccgaga
     4201 agagagccca cgatcggata cagaatgagc gcatagagaa tcaagagacc tccggcccat
     4261 ccccgaacat cccatgagaa gcgaaactct agagttctgc gaaatccatt ccagagaaag
     4321 atcaagccct gaagtatgca ggcaatccca aagacgtagg ccgcaggatt aatggaagta
     4381 aactgaacga tgtggtaggc gatccccatc cagatccaaa ggacaccgag tatggaacca
     4441 ctcataatcg acgagaacga gaacgttttc aaagacagag caaggatcac gagcgcgact
     4501 gcaaagagcg ccacctgtgc cggccacacg ctttgattgt aagcggcgaa cactccaaga
     4561 aattgttcag ttgtgaatgg aattttcata aatcagtgcg ccccttcgtt cgaatcattg
     4621 aatcccttcg cactcgccaa ccctgggccg agaagcttac gaacaatcta ctttgaatcc
     4681 tgttctcgct caatgcggaa gtgaacaaaa ttttcccggg gcggataacc ttccagtggc
     4741 gcgtcgccgg tgcctctcga cccaagcttt gatcgtggaa agccttcgtc cactaggcca
     4801 ttgagtacaa cacgagcaat ctgttcacta caggattgtg cttccgcgtc ttgtgtcgcc
     4861 gcaaagcagt gccccaccac caccatgatg tatcccggag ccaatgcgga tcgcacctgg
     4921 gtcatgtggc cggcagaata ccatgcatga aattctggtc cgacagattc agcattttct
     4981 gatagggact gactggcggg aggaaatcga aggggactga cggcggctgt ggattggtgt
     5041 gcc
//
LOCUS       DAORFF010000257         5035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9359_length_5035_cov_4.086747, whole genome shotgun sequence.
ACCESSION   DAORFF010000257 DAORFF010000000
VERSION     DAORFF010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9359_length_5035_cov_4.086747"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PKK76_11005"
     CDS             <1..372
                     /locus_tag="PKK76_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNL69047.1"
                     /translation="ADVDVVEDLHGTSRKHPAVALGFTVVLCSSAGVPLTAGFIAKFG
                     VIYSMLQGPEKLPGLAAVAGALCSVLAFYYYFKIIRAIWFESEIVTQVREQKRAYLIV
                     CVLLVCALLAGGIYPPALTWI"
     gene            complement(369..875)
                     /locus_tag="PKK76_11010"
     CDS             complement(369..875)
                     /locus_tag="PKK76_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNL69048.1"
                     /translation="MVGQSEFEDGFEDAALPGEFLRMTLKELPRRPLLSLPPTATIAD
                     AIQAMNDKRVGAVLLMEGNTLAGIFTERDVLKRVALSGIDVRTTSVSTVMTKKPDMLP
                     SDAGVAYALRKMNEEGYRHIPIVDKNGKATGIVAVRDIVRWLVELFPEELNLPPDPSL
                     ESRSVDGG"
     gene            complement(967..2211)
                     /locus_tag="PKK76_11015"
     CDS             complement(967..2211)
                     /locus_tag="PKK76_11015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HNL69049.1"
                     /translation="MRISADKRMPQRALTFLFFTVLIDVIGFGVVLPVTPGLIMELNG
                     TDLSSASTVGGWLLFAFAITQFFFAPIMGNLSDRFGRRPVLLSSLAAFGLDYTITGFS
                     PNLSWLFVGRIIAGATGASFSTANAYIADISKPEDRAKNFGLIGAAFGLGFILGPALG
                     GIVGGFGTRIPFFVAGGLAFANALFGAFALPESLPESSRRLFQWARANPAGTLIHLKK
                     YPMIYALAAVNFLWQLAHQVLPSTWTFYTMLKFNWNQNDVGYSLAFVGLCMTIVQGGL
                     SGPIIKKLGERGAAIGGLLIGAIGFFGYALSPSPWIIYIFAAISSLSGLTYPSLNALM
                     SGQVPATSQGELQGGNSSLISITSILGPLLMTQTFHMFATGPGIVFPGAPFALAGILA
                     IGALMVLFISLARIARAPGPQG"
     gene            2341..3750
                     /gene="sufB"
                     /locus_tag="PKK76_11020"
     CDS             2341..3750
                     /gene="sufB"
                     /locus_tag="PKK76_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNL69050.1"
                     /translation="METSVQSALDFYEPDNFPSGISRKVVESISHIKNEPGWVTEFRL
                     RALETYMSRPMPSWGFIPQFKIDLDSYVHYVGSRQKKKKSWDEVDPEVLRSFERLGIP
                     EHERRYLAGLEAMNDSETVYANVKKELADLGIIFCDIDTAIREYPEIVKKYIGSVVTP
                     EDNKFAALNSAVFSGGSFAYCPRGVKTPMPLQAYFKVTAASSGQYERTLLIADEGSEI
                     VYSEGCSSVQDSGTNFHTAVVELVAHKDARIHYTTIQNWKKNMYNWTVKRGIAHESAH
                     ITWTDVNIGAQTIKYPGIILAGDHATGDVLSLAFAGGGQIQDTGARIIHVGKNTRSNV
                     LAKGVSLDGGINSYRGLVKFEKGATGAMSHVKCDGLMMDARSESHAYPYNDVSGENGQ
                     LNYEATVSRINDDQLFYLQSRGLSEDEAKLLIVSGFCEGVVRDLNVEYSVEMTRLIRM
                     ILEDGKAIAAPEQAAAALP"
     gene            3771..4511
                     /gene="sufC"
                     /locus_tag="PKK76_11025"
     CDS             3771..4511
                     /gene="sufC"
                     /locus_tag="PKK76_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020784755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNL69051.1"
                     /translation="MLLEIKGLEASIEGKEILKGVNLSIPPGEIHAIMGPNGSGKSTL
                     SYVLMGHPKYTITGGDILLDGKSVLAMKPDERARAGMFLCFQYPTSIPGVTMPNYLRN
                     VLKNIRGADVPIKEFRKEVQDSMRNLSMREDFLKRYVNDGFSGGEKKRNEILQMALMK
                     PRVAILDEVDSGLDIDALRIIAENLQAMRSPDRSMLLITHYQRILNYLTLDKVHVFAE
                     GRIITSGGRELAERLEKEGYEAVIKANK"
     gene            4508..>5035
                     /locus_tag="PKK76_11030"
     CDS             4508..>5035
                     /locus_tag="PKK76_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69052.1"
                     /translation="MTTLIAEKREEARRLLPEMHLPSRQHESWRRVNLGALDIKRYTT
                     TGTIIEILEKRGAEILEGDAAYSQAILWEGAPEDLFDCANLAEAKTILIKVETSAHVR
                     IKHSMGSNANGLHHRIYVMVPEGADASIIEETQGRPGDSTFWNLHTEIYAGAGSSVRH
                     LSIRGYEGNEWNLHRL"
BASE COUNT         1317 a         1217 c         1331 g         1170 t
ORIGIN      
        1 gcggacgtgg acgtagtaga agatttgcat ggaacatctc gcaaacatcc tgcggttgct
       61 cttggtttta ctgtggttct ttgtagttcc gcaggcgttc cgctgactgc aggattcatt
      121 gcgaaatttg gagtgatcta ctctatgctg caggggccag agaaacttcc aggcctcgct
      181 gcggttgccg gagctctctg ttccgtcctt gcattttatt actatttcaa aatcatacgg
      241 gccatctggt ttgagtctga aatcgtaacc caggtgcgtg agcagaagcg agcctacttg
      301 atcgtatgcg tgctgcttgt ttgcgcattg cttgcgggag gaatctaccc acccgcactt
      361 acctggattt aaccgccgtc tacagatctt gattcaagag aagggtccgg tggtaagttg
      421 agttcttccg ggaagagttc tacaagccat ctcacaatgt ctcgaaccgc cacgattccg
      481 gttgctttac cgtttttgtc tacaatcgga atgtgtctgt aaccttcttc gttcattttt
      541 cgaagggcat acgcaacccc tgcgtcgctc gggagcatat ctggcttctt tgtcataaca
      601 gtggaaactg aagttgtgcg cacgtcgatt ccagagaggg caacacgctt gagtacatcg
      661 cgctccgtaa aaatccccgc cagagtattt ccttccatta acaaaactgc gccgactctc
      721 ttatcattca ttgcctggat tgcatctgcg atcgtggctg tgggaggaag agaaagaaga
      781 ggccgtcgcg gtagctcttt cagagtcatg cgcagaaatt caccgggcag tgctgcgtcc
      841 tcaaacccat cttcgaactc gctttgacca accatgtccc catggtagaa ctcattgatg
      901 caaacggtca agtattcctg agcgtttttt tggacgggtt ctagaaaatc cgtctgtagt
      961 tcagagtcag ccctgtggtc ccggtgcgcg cgcgattcgt gcgagtgaaa taaaaagaac
     1021 catgagcgcg ccgattgcca gaattccagc cagtgcaaat ggagcacccg gaaaaacgat
     1081 tccgggacct gtggcaaaca tgtggaaagt ctgtgtcatt agaagcggtc cgaggatgct
     1141 tgtgatgctg atgaggctgg agtttccacc ctggagttcc ccttgcgaag ttgccgggac
     1201 ctgaccggac atcagtgcgt tcaaggatgg ataggtgaga ccactgagag aagaaatcgc
     1261 tgcgaatata taaatgatcc agggagatgg agaaagtgcg tatccaaaaa aaccgatcgc
     1321 accaatcaaa agacctccga tggcagcgcc acgttcaccg agtttcttga tgattggacc
     1381 gctgagtcca ccctgaacaa ttgtcataca gagtcctacg aaggccaggg agtatcccac
     1441 atcattttga ttccaattga acttaagcat cgtatagaaa gtccaagtgg aaggaagtac
     1501 ctgatgcgca agttgccata ggaaattgac tgcagcgagt gcgtagatca ttggatattt
     1561 cttgagatga atgagtgtac ccgctggatt tgctcgcgcc cactgaaaaa gtcttctgga
     1621 ggattccggc aaagactctg gaagagcgaa ggctccgaac aatgcattgg caaatgcaag
     1681 tccacctgcc acgaagaagg ggattcgcgt tccaaatcct cccacaatgc cgcctaacgc
     1741 tggacccaga atgaaaccca gcccgaatgc cgcgccaatc agtccgaagt ttttcgcgcg
     1801 atcttctggc ttgcttatgt cggcaatgta tgcgttcgcc gtggaaaagg acgcgcctgt
     1861 ggcgccagcg atgattcgtc cgacaaagag ccaggacaga ttgggcgaaa agcctgtgat
     1921 ggtgtagtcg aggccgaatg cagccagaga gctcagcaaa accggtcgtc ttccgaatcg
     1981 atctgataga tttcccatga tcggtgcaaa gaagaattga gtgatggcaa atgcaaagag
     2041 cagccagccg cccacagtgg aggcactgga tagatccgtg ccattcagtt ccatgatcag
     2101 tccgggtgta acaggcagta cgacgccaaa accgatcaca tcgatgagga ccgtgaagaa
     2161 gaggaaagtg agcgctcttt gcggcatacg tttgtcagca ctgatacgca ctgatttgca
     2221 tgcaacgatt tcggcggcga aatcgagtcc ctggtgcctc gaaagcacac atctgacggg
     2281 caaaacgatc cattcaaaaa aggctttgtt tggagccttt cttcaatttt actgtcggta
     2341 atggagacat cagtgcaatc agcgctcgat ttctacgagc cggacaattt tccgtccggg
     2401 atctcgcgga aagtggtaga gtctatctct cacatcaaaa acgaaccggg ctgggtgact
     2461 gaattccgtc ttcgcgcact ggaaacatac atgtccaggc caatgccttc gtggggtttc
     2521 attcctcagt tcaaaatcga cctggattcc tacgttcact acgtgggcag tcgccagaag
     2581 aaaaagaaat cgtgggacga agtggatcca gaggtactgc gtagctttga gagacttgga
     2641 atccctgaac atgaacgcag ataccttgca ggcctcgaag caatgaacga ttcggagact
     2701 gtgtacgcga acgtaaagaa agaactggca gatctgggaa tcatcttctg tgacattgat
     2761 acagcaattc gtgaatatcc tgaaatagtt aagaaatata taggaagcgt agtcactccg
     2821 gaagacaata agtttgccgc actcaacagt gcagtcttta gcggcggatc ctttgcgtat
     2881 tgcccgcgcg gagtgaagac tccgatgccc cttcaagcat attttaaagt gacggcagca
     2941 tcctccggtc agtatgaacg gactcttctc attgcagatg aaggatcaga aattgtgtat
     3001 tcggaagggt gtagctccgt tcaggattcg ggaacaaact ttcacaccgc tgtggtggag
     3061 ctggtcgctc acaaggacgc gcgcattcac tacactacaa tccagaactg gaaaaagaac
     3121 atgtacaact ggactgtgaa gcgtgggatt gcgcacgaat cagcgcatat cacctggaca
     3181 gatgtcaaca ttggcgcaca aaccatcaaa taccccggca tcatactggc aggtgatcac
     3241 gcaaccggag acgttctttc tctcgctttt gccggaggtg gccagattca ggacacggga
     3301 gctcgcatca ttcatgtggg aaaaaataca cgctccaatg ttcttgcgaa gggtgtttct
     3361 ctcgacggcg gaatcaattc gtatcgcggt ctggtaaaat tcgaaaaggg cgccacggga
     3421 gccatgagtc atgtaaaatg cgatggcctg atgatggacg cgcgatctga atcgcacgca
     3481 tatccgtaca acgatgtgag cggagaaaat ggtcagttga attacgaggc taccgtttca
     3541 cgtatcaatg acgaccagtt gttctatctg caatccaggg gactctcaga ggatgaagca
     3601 aaattgctca tcgtaagcgg cttttgcgaa ggcgtggttc gcgatctcaa cgtagagtac
     3661 tcggtggaga tgacgcgctt aatcaggatg atcctggagg atggaaaagc gattgcggct
     3721 cctgagcagg ccgccgctgc cctaccttga atcaataaag gaattttagt atgcttctcg
     3781 aaatcaaagg cctcgaggcc tccatcgaag gaaaggagat tctcaaaggt gtgaatcttt
     3841 ctattccgcc cggagaaatc cacgcgatca tgggaccgaa tggttctgga aagagtacct
     3901 tatcctacgt gctgatgggt catccaaagt acacgatcac cggcggagac attctcctcg
     3961 atggcaaatc cgtccttgcc atgaagccgg acgagcgggc cagagcaggc atgtttcttt
     4021 gctttcagta tccaacgtcg attccgggcg tcacaatgcc caattatctg cgaaacgttt
     4081 tgaagaatat tcgcggcgcg gacgtcccca tcaaagagtt tcgcaaagaa gtccaggact
     4141 ccatgcgaaa cctctccatg cgtgaagatt ttcttaagag atacgtaaac gacggctttt
     4201 caggcggaga aaagaaacga aacgaaatcc tgcagatggc tctcatgaag ccgcgtgttg
     4261 ccattctgga tgaagttgat tccgggttag acattgatgc gcttcgcatt atcgcagaga
     4321 atctgcaggc aatgcgttca ccggatcgct ccatgctact cattacacac taccagagaa
     4381 ttttgaacta tcttacgctg gataaagtgc acgtctttgc agaggggcgg atcatcacat
     4441 ccggcgggcg ggaattggcg gagcgtctgg aaaaagaagg ctacgaagcc gtcatcaaag
     4501 caaacaaatg actacgctca ttgcggaaaa acgtgaagaa gcacgacggc ttcttccgga
     4561 aatgcatctt ccatcgcgcc agcatgaatc ctggagacgt gtgaacctcg gtgctctgga
     4621 cattaaacgt tatacgacaa ccgggacaat catcgagata ctggaaaagc gcggagcgga
     4681 aattctggaa ggcgatgccg catactccca ggcgattctc tgggagggcg cgcctgagga
     4741 cctctttgac tgcgcgaatc tggcagaagc aaaaacaatt ctgatcaagg tggaaacatc
     4801 cgctcacgtt cgcataaagc attcgatggg ttcgaacgca aacggccttc atcatcgcat
     4861 ctatgtaatg gtgccagagg gggcagatgc ttcgatcatc gaagaaacgc agggtcgtcc
     4921 gggggattct actttctgga atttgcatac ggagatctat gcgggggcag gatcgagcgt
     4981 tcgccatctt tctattcgag gttacgaagg gaatgaatgg aaccttcatc gcctc
//
LOCUS       DAORFF010000258         5013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9421_length_5013_cov_4.726906, whole genome shotgun sequence.
ACCESSION   DAORFF010000258 DAORFF010000000
VERSION     DAORFF010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9421_length_5013_cov_4.726906"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PKK76_11035"
     CDS             <1..853
                     /locus_tag="PKK76_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69053.1"
                     /translation="VKTIVTASNSVTIDDLPAGSYRWRIRTKPNQPDLNSRVSQSGSF
                     EVYKTDLVPPETASPFMSEHLTVSQAKEGIFFNWRAPGEMKSFVLQISQNPGFGNSLM
                     NQQTEQNFSVVKGMPEGIFYWRVRGTTASGKETPYSVVQSFTIGDKYMKSEIETPDAT
                     RVHVLFPLRQAVDMTGRKSIDFKWEAVPGARKYNIRLYRNLGGKYEPIYAGSTTATGF
                     SYTDMKSLDIGYFAWEVQAEGGTLQPSGRIDFSITLNRYLQNLKPGDIQFVNPENIYR
                     EEQNDSK"
     gene            840..1910
                     /locus_tag="PKK76_11040"
     CDS             840..1910
                     /locus_tag="PKK76_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69054.1"
                     /translation="MIVNKYLAFALLLLAAAPLFAGPDDYVEWKPIPGSSGYQLQVRD
                     AKTKRIMVDQFVEGTYLDVDLQVGHFEQRLAPLSPFGKPLIWSDWRPLNILVALSPVV
                     DEANAVEVKERRPFSMFLRGKNFTPNTRVFLKKDGNSIPVPNRSINAAGTELTLLVNP
                     ATIPPGNYSLYLENPRQKTWTGESLVSLKYAENAVAVVDPKDQKDVKNPLDQKDPRTQ
                     KNQRDQKDPKNQNKTQQNKSEVTGDFRDHLRTMPSTCKNSGLPDELVLKCYKRYIVLN
                     LSTPAKVELFNYLMVYQGNYDDRMRGYSAFRDSCNSMSRHTESLLRERLAQSGESLDL
                     AEKQQIQGTLSFIHNCNASSNP"
     gene            1891..3201
                     /gene="hisD"
                     /locus_tag="PKK76_11045"
     CDS             1891..3201
                     /gene="hisD"
                     /locus_tag="PKK76_11045"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNL69055.1"
                     /translation="MPLRILDESSIDSASRARLIERAYAQDRSAFAAAQGAIDDVEVR
                     GADAVREFTKKFDGVDLSDILVSEEEWARGDLTASEVQDAFTKAYENILAFHRLQLDS
                     LKGARETIGGTVLGFRYQAVTTAAMYVPGGKASYPSSVLMGLIPAVVAGVKNRIVVTP
                     PDKDGTLLREILFCAKLAGATQIIKAGGSQGIAAAAFGHNAPGLRTPPAELIIGPGNR
                     YVTAAKSILASRGLVRIDSPAGPSEVLIIADHTARPDFVAADLLSQAEHGEDSAAVLC
                     CLSRKFAQEVSAEIERGFRERPARRDMKEKSIRDHSFAIVFESLENAVAFSNDFAPEH
                     LEICTENPEKVFEGIVSAGSVFLGHFAPVALGDYFSGTNHVLPTGRAAASYSGLGVDT
                     FLKRITYQHPTRESLKEALLPIQVMSKVEGLEHEHGHSVEIRFH"
     gene            3245..3622
                     /locus_tag="PKK76_11050"
     CDS             3245..3622
                     /locus_tag="PKK76_11050"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNL69056.1"
                     /translation="MNSVAYFEIQSDNPAKSAEFYGSIFGWTCTHAPEMPIEYWRVET
                     AGIRGGILKRPARIPPPQSGTNAFCCSMEVKNFDSVATQILDRGGQVALPKFAVPGVC
                     WQGYFIDPDGNTFGLFQADSNAK"
     gene            complement(3629..4402)
                     /locus_tag="PKK76_11055"
     CDS             complement(3629..4402)
                     /locus_tag="PKK76_11055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL69057.1"
                     /translation="MNCPHTGQALREILGGRAFQNEQGVIYVKGTRSLDYGADYFLTE
                     YEKQYGKTYLQDEPNLRRLAARRLEDLSRVAPPPASLFEIGAACGFFLDEARICGYDV
                     HGLEISEFASNHARSLGLDVQTASFPTATNKQYDVVAAFFVLEHFPDQKSAFESVSKM
                     IRPGGIFAAALPSSHGPTLKYSPDDWIRTHPEDHFADYSPRSLAKILPLYGMELINAR
                     PASFHPSRARGILRWPPLYRLYARANSFGDTMEILARKK"
     gene            complement(4399..>5013)
                     /locus_tag="PKK76_11060"
     CDS             complement(4399..>5013)
                     /locus_tag="PKK76_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69058.1"
                     /translation="ACLVFLRACWLILQKQDDRLRVILCFFAVAGFALFSSEGWYVVY
                     GDAFLILGAAGVYRMADFRAARFLLLATALTMSGLLCYFTIRHKLLDTTRHAREMSSA
                     MMKAMSGCKAVYLRARPDPYFDLRNTQIRTYEFVPGKLKIKPERINDLKKTWDSIDCF
                     LLERNPTWEPLLTEYLEQRKGEFRITSLEAPYPLYSSDLYRRDR"
BASE COUNT         1254 a         1230 c         1308 g         1221 t
ORIGIN      
        1 tgtgaagacc atcgtgactg cttctaattc agtaacgata gacgatcttc ctgctggaag
       61 ttatcgttgg agaattcgca cgaagcctaa ccagccggat ttgaattcgc gcgttagcca
      121 gagcggatcg tttgaagttt acaaaaccga tctggtcccg ccggagacag cgagtccttt
      181 catgtcagaa catctgacag tctctcaggc aaaagaagga atcttcttta actggcgagc
      241 acccggggaa atgaaatcat ttgttctcca aatttctcaa aaccctggat ttgggaattc
      301 cctcatgaac cagcagacgg agcaaaactt ctccgtggtc aaaggaatgc cagagggaat
      361 cttttactgg cgcgttcggg gcaccactgc atctggcaaa gagactccct attcagtagt
      421 gcaaagcttt acaatcggcg acaagtacat gaagagtgaa atagaaacac cggacgcgac
      481 tcgcgttcat gttcttttcc ctcttcgcca ggcagtggac atgactggcc gcaagagcat
      541 tgacttcaaa tgggaagcgg ttccaggcgc gcggaagtac aacattcgcc tgtatcgaaa
      601 cctgggcggc aagtacgagc ccatttacgc gggaagcaca actgccactg gtttttcgta
      661 cacggacatg aagtcgctgg acattggcta tttcgcatgg gaagttcagg cggaaggtgg
      721 cacgcttcag cccagcggtc gaattgactt ttctatcact ttgaatcgct atttgcagaa
      781 tctaaagcca ggcgacatcc aatttgtgaa tccggaaaac atatacaggg aagagcagaa
      841 tgatagtaaa taaatactta gcatttgcac ttttgctcct ggcggcggca ccgcttttcg
      901 caggtcccga tgactatgtg gagtggaagc ctatccctgg ttcttccgga tatcagctgc
      961 aggtgcgcga tgctaaaaca aagcgcatca tggtggatca atttgtggag ggaacatatc
     1021 tcgatgtaga tctccaggtg ggtcacttcg agcagcgtct ggcaccgctc agcccgtttg
     1081 gaaagcctct catctggtcc gactggcgcc cgctcaacat tctcgtggca ctttcccctg
     1141 tggtggatga agcaaacgca gtggaagtaa aagagagaag gccattcagc atgtttttgc
     1201 gcggaaaaaa ctttacgccc aatacgcgcg tcttcttgaa gaaagacgga aacagcattc
     1261 cagttcccaa tcgcagcatc aacgccgccg gcacagaatt gactcttctt gtgaatccgg
     1321 ccaccatccc tccaggaaat tacagccttt atcttgagaa tccgcgccag aaaacctgga
     1381 ccggagaaag tctcgtcagt ttgaagtacg cggagaatgc agtggcggtc gtcgatccaa
     1441 aagatcagaa ggatgtaaag aacccgctgg atcaaaaaga cccgcgcact cagaagaatc
     1501 agagggatca gaaagatcca aagaatcaga acaaaaccca gcagaataaa tctgaagtca
     1561 cgggggattt ccgtgatcat cttcggacca tgccgtctac atgcaagaac tccggtctgc
     1621 ctgacgaact cgttttgaaa tgttacaagc gatacattgt cttgaattta tcaacacccg
     1681 ctaaggtaga actgttcaat tacctgatgg tctatcaggg caactatgac gatcgcatgc
     1741 gcggctacag cgcgtttcgc gatagttgca actccatgtc cagacacact gaatctctct
     1801 taagggaaag gctcgcacaa tcgggcgaat ctctcgatct ggcagagaag cagcagattc
     1861 agggcacact ttcgttcatc cacaactgca atgcctcttc gaatccttga cgaatccagc
     1921 atagactctg cttcgcgggc caggctgata gagcgcgcat acgcgcagga tcgttccgct
     1981 ttcgctgcag ctcaaggtgc gatagacgat gtggaagtgc gcggtgcaga tgcagtgcgc
     2041 gaatttacaa agaagtttga tggagtagat ctttcggata ttctagtatc tgaagaagag
     2101 tgggccagag gggacctcac cgcgtctgag gtgcaagatg ctttcacgaa ggcttacgag
     2161 aatattctcg cattccatcg tctgcagctg gactctctca agggtgctcg cgaaacgata
     2221 ggcggcactg tacttggctt tcgatatcag gccgtgacta cagcggccat gtatgttcct
     2281 ggagggaaag caagttatcc cagttctgtt ttgatgggtc tgattccggc agtcgtagcc
     2341 ggagtgaaga atcgaattgt ggttacacct cctgacaaag acggaactct attgcgcgaa
     2401 attcttttct gtgcgaagct tgccggcgcc acgcaaatca tcaaggcggg tggttctcaa
     2461 ggaattgcgg ccgcggcctt tggacacaat gctcctggtc tgcggactcc acccgcagag
     2521 ctcatcattg gtccgggaaa tcggtatgtc acggcggcaa aatcaattct agcttctcgc
     2581 gggctcgttc gcattgattc gccggccggt ccctctgaag tcctgatcat cgctgatcac
     2641 actgctcgac ctgattttgt cgctgcagat cttctcagtc aggctgaaca cggagaggat
     2701 agcgccgctg ttctatgctg tctttctcgc aaattcgcgc aggaagtaag tgcggagatt
     2761 gaacgtggat ttagagaacg tccggctcgt cgtgacatga aggaaaaaag cattcgcgat
     2821 cattctttcg caattgtatt tgaaagcctg gaaaatgcgg tcgctttttc caacgatttt
     2881 gcgccggagc atctggagat ttgcacggag aatccagaga aggtttttga aggaattgtt
     2941 tctgcgggca gtgtattcct tggacatttc gctcctgttg ctctcgggga ttattttagt
     3001 ggaaccaacc atgttcttcc tactggtcgc gcggctgctt cctattccgg tctgggcgtg
     3061 gacacattct tgaagagaat tacataccag catccgacgc gtgaaagtct gaaagaggcg
     3121 ctccttccca ttcaggtgat gagcaaagtg gaaggtctcg aacatgagca cggtcactct
     3181 gtagagattc gctttcactg actctttgaa ttgacgctcc ctggaatgtc tccattcttg
     3241 agcggtgaac tcagttgctt attttgaaat acaatccgac aacccggcaa agtctgccga
     3301 attttatgga tctatctttg gatggacatg cacgcacgcg ccagaaatgc ccatcgagta
     3361 ctggcgcgta gagactgctg gaattcgcgg cgggattcta aaaagacccg caaggattcc
     3421 tcctccgcag agcggaacga atgctttctg ctgttctatg gaggtaaaga attttgattc
     3481 tgtggccact caaattctcg atcggggcgg tcaggtcgca cttccaaaat tcgcagtgcc
     3541 aggtgtttgc tggcagggat acttcatcga tccagacgga aatacgtttg gtttgttcca
     3601 ggcggactca aacgcaaagt aactgcacct atttctttcg cgccaggatt tccattgtgt
     3661 ctccaaagga atttgctctg gcgtagagcc tgtagagtgg tggccagcgc aagattccgc
     3721 gcgcgcgaga cggatgaaaa gacgctggtc gcgcgtttat cagttccatg ccgtataacg
     3781 gtagtatctt tgcaagactg cgaggagagt agtcagcgaa atgatcttct ggatgagttc
     3841 gaatccagtc atccggcgag tattttaaag taggtccatg gcttgaggga agggcagcgg
     3901 caaagattcc accggggcga atcattttgc ttacagattc aaacgcactc ttctggtccg
     3961 gaaaatgttc cagaacgaag aaagctgcga ccacatcgta ttgtttgttt gtcgctgtcg
     4021 gaaaggaagc tgtttgcaca tcgagcccca gactccgtgc atggttggag gcaaactcag
     4081 agatttccag accatgtaca tcgtatccgc atatgcgtgc ttcgtctaaa aagaagccac
     4141 aggcagctcc gatttcaaac aaagatgcgg gaggaggcgc tacgcgggaa agatcttcca
     4201 gtcttcgcgc agcaagtctt cgcaggttgg gttcatcctg taaataggtc ttgccatatt
     4261 gcttttcgta ttccgttaga aaatagtctg cgccgtagtc cagactgcgg gtgcccttaa
     4321 cgtagatgac cccttgctca ttctggaagg cacgtccgcc tagaatttct ctgagagcct
     4381 ggccggtgtg cggacagttc accgatctct ccgatacagg tcagacgagt agagcggata
     4441 gggcgcttcc agggacgtta tgcggaattc tccctttctt tgttccagat actccgtaag
     4501 caatggttcc cacgtgggat ttctttcgag taaaaagcaa tcaatcgagt cccaggtctt
     4561 tttcagatcg tttatacgtt ccggtttgat tttgagttta cccggtacga attcgtaggt
     4621 tctgatttgt gtattgcgca gatcaaaata tggatcaggt ctcgcgcgaa gatacactgc
     4681 tttgcagccg ctcattgctt tcatcatggc ggagctcatc tcgcgcgcgt gacgcgtcgt
     4741 atccagcagt ttgtggcgaa tggtaaagta gcagagaagg ccggacatcg taagggccgt
     4801 tgcgaggagc aggaatcttg ccgcccgaaa gtccgccatg cgatagactc ctgctgcgcc
     4861 tagaatcaga aacgcatcac cgtaaactac ataccaccct tcggaagaga agagcgcgaa
     4921 accagcaact gcaaagaagc aaaggatcac acgtagcctg tcgtcctgct tttgcagaat
     4981 caaccagcat gcgcgcagaa aaacaaggca ggc
//
LOCUS       DAORFF010000259         4959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9570_length_4959_cov_4.243067, whole genome shotgun sequence.
ACCESSION   DAORFF010000259 DAORFF010000000
VERSION     DAORFF010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9570_length_4959_cov_4.243067"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKK76_11065"
     CDS             complement(<1..284)
                     /locus_tag="PKK76_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69059.1"
                     /translation="MQSEHDDKIPLDLVVQGAVFEYRRAFSRAEILAFADLSGDHGEH
                     HVHGDRLMAHGLLVASIVTKIGGDLNYISQEILLRFEAPVYEGEEVTGRIT"
     gene            complement(355..702)
                     /locus_tag="PKK76_11070"
     CDS             complement(355..702)
                     /locus_tag="PKK76_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNL69060.1"
                     /translation="MSEISVAHGDVLFVVARAKALEVQEIAAALGKDKSTVSSIVSQL
                     EAHGYLARARDPGDARRAIIRLTPLARRITPRMLAISRVFNARLLTGISRQERKFLEE
                     LLRRMDTNLAELE"
     gene            1151..1510
                     /locus_tag="PKK76_11075"
     CDS             1151..1510
                     /locus_tag="PKK76_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69061.1"
                     /translation="MNFKRTLLSGLCLLLLGCATTATQQVDIPVYFGPRPGAPASKDF
                     NNIAARDSFLLVGVRNSAGADQKSEVEKVLINNTKSTKDWATIQEVDVSGYTIIILPW
                     MFGTAENSVKGKVEHAQ"
     gene            1500..1934
                     /locus_tag="PKK76_11080"
     CDS             1500..1934
                     /locus_tag="PKK76_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69062.1"
                     /translation="MRNRISFFVFVAIFASVCTSCMVGGRGTATFDQVRYPISGTTTL
                     LKKDGTTLNAASAGKPLGHVQAHKFYWAMLYGSVSLTGKYDLSQDMNAAVEEVGGNAV
                     INAKLTNTQCPTNYVLVVLGILPFIPGCVSVTLEGDAVKLDL"
     gene            1948..2244
                     /locus_tag="PKK76_11085"
     CDS             1948..2244
                     /locus_tag="PKK76_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69063.1"
                     /translation="MLVLLLASCATPQAPVLTEEEKKIVVLQATGVKFLEMNCKPLSG
                     VEDYAFRESAIQAAHRRRATHVQLLPLASGMSSTGGFATSYQSFSYRAWSCISP"
     gene            complement(2273..2563)
                     /locus_tag="PKK76_11090"
     CDS             complement(2273..2563)
                     /locus_tag="PKK76_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69064.1"
                     /translation="MNPEEIRAAFDRIKRRNLFAGFVPLVFPFFAFAIYQKDLFKQPE
                     ILFVSILPALAAAVWVHLVNWRCPACRAWLGRFFTVSECPSCKVKLTLKLNT"
     gene            2819..>4959
                     /locus_tag="PKK76_11095"
     CDS             2819..>4959
                     /locus_tag="PKK76_11095"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL69065.1"
                     /translation="MKHFIRLTLLLYFVSALPAAPVHMTDEEVPVSGTSLQIIEDKLA
                     ALDLTNLPEGLWKSSHNGDFGAGFTEAVYWLKFSAVNMRPEENWTLHIENALVDELEI
                     WKTGGESAHIQTGTKIPYKDRELAGRLFHLPVKIKKGETANFLIRAKMRASMNLPIRL
                     RTDRVTASWNNRENLILGLFHGAVLIMIVYHAFIWLSVRDRIYAYFILVLLFGSTFMA
                     AQNGLAFQYLWPGIGERGVQINLTFGVLTLLTSSLFIREMLNLRARWLPGFFAANGLI
                     ILSAICLAAVWIVSYKIAASTMTTLTLMQAMVAMSVGLRFALRRDRIAAWFASAYFVV
                     IASSDLFALRSVGVIPPSQWITHSLMIGILMQNILLSLSLAERINVLHLEAEVSKQAS
                     ELKTKFMATMSHEIRTPMHGVIGLTNLLLDTQLDAEQRMLATQTSRSAQALLEIINDV
                     LDFSKIEAGRLELHPETTRITDPVASVVNLMQPLAQKKGLHIRTSLSREIPPFVSVDA
                     GRLRQILLNLVANAVKFTSEGEISLTGGYEQGNIFFSVRDTGIGIDSEYLPHLFDPFY
                     QVDSRLTRQFQGTGLGLAITKHLVTLMGGTIRVESEPNVGSVFEFRLPAPAVAAPTEI
                     RKPDESMVGNLRVLVADDDEVGRLVGGQYLRKLGCTVDLAEDGPAVLRCMQSQKYAAI
                     FLDMQMPHLDGPDTAREIRKLYGDTPFLIALT"
BASE COUNT         1243 a         1324 c         1245 g         1147 t
ORIGIN      
        1 gtgatcctgc cagtcacctc ttcgccctcg taaacgggtg cttcaaatcg cagtagtatt
       61 tcctgggata tatagttcaa atcaccaccg atctttgtca cgatactcgc caccagaagt
      121 ccatgcgcca tcaaccgatc tccatgaaca tgatgttccc cgtgatcgcc cgaaaggtct
      181 gcaaacgcca gtatttctgc gcgtgagaaa gctcgccggt attcaaaaac agctccctga
      241 accacaagat ccagaggaat tttgtcatca tgttccgatt gcattggcgg ctccaatagt
      301 tttgtacaaa accattttcc gtgagtcacg ctcgtcaaca agctttcttc gtttctattc
      361 cagttccgcg agattggtat ccatacgccg cagaagctct tccagaaact tgcgttcctg
      421 cctggaaata ccggtcagaa gacgggcgtt gaaaaccctt gaaatggcaa gcatccgtgg
      481 cgtaatccgc cgcgcgagcg gagtaaggcg aatgattgca cgccgggcat cccctggatc
      541 gcgcgccctg gccagatacc cgtgcgcttc caattgagaa acgatggaac tgacggtgga
      601 cttatccttt ccaagggccg ccgcgatctc ctgaacttct agagcttttg cgcgcgccac
      661 aacaaacaat acatcgccgt gcgcgacgga aatctcggac aatcccgctt cctccagatg
      721 cttttcaaga tagcggaaag ctgcggatcg aacccggctc aggaggtgca gtaaagaagt
      781 tttcaaatgg tagctcgatc agtgaacaaa aacaaagaac aagacgaggg ctgtcagctt
      841 ctggtcatcc aggaatcgct ctgcattcaa gctcgccact gcgaagctcc gcgcaatcaa
      901 aaaagatccg ctccatgacg atatcaagga tgttgctttg aaagagatag tccacctccc
      961 gaggtcgcga gatgaatgca ctaacattcg aatgaaagct acgtaaagcg taatgaacaa
     1021 cacaaagatc gtgtgcagaa cgaatcagtt aaagaaaatc aagtatacgc gcaggaggat
     1081 cgagtctctt caaaaaatga ttgaccgcat tggcgcctaa cggtccttta tgccgcggga
     1141 gaaccagcca atgaacttca aacgaacgct tctttccggg ctatgcctgc ttctactcgg
     1201 ctgcgccact acagcaacgc agcaggtcga cattcccgtc tatttcggac cacgccccgg
     1261 tgcgcccgca tccaaagact tcaacaacat cgccgccaga gattcttttc tattggtagg
     1321 cgtgcgaaat tcagccggag cagaccagaa aagcgaagtt gaaaaagtac tgatcaacaa
     1381 tacgaaatct acaaaagact gggccaccat tcaagaggtg gatgtctccg gttacacaat
     1441 tatcatctta ccgtggatgt ttggtactgc ggaaaattct gtgaaaggaa aggtagagca
     1501 tgcgcaatag aatttcattt tttgtattcg tcgcgatatt cgcgtcggtt tgcacttcct
     1561 gcatggtcgg cgggcgaggt accgcgactt tcgatcaggt tcgctatccg atttctggca
     1621 ctacaacctt actgaaaaag gacggtacca ccttgaacgc ggcctccgcc ggcaagccac
     1681 tcgggcatgt acaggcacac aagttttact gggccatgtt gtacggatcc gtttcgctga
     1741 ccggcaaata tgacctcagc caggatatga atgcggcggt agaagaagtg ggcggcaatg
     1801 cagtgatcaa cgccaaactc acgaacacac agtgccctac caattatgta ctggtagtgc
     1861 tcggaattct cccgtttatt ccgggttgtg tttccgtcac gctggaaggc gacgcagtaa
     1921 agcttgacct ttgattgcag acggtgcatg ctcgttttgc tgctcgcgag ctgcgcaacc
     1981 ccgcaggcgc ccgttctaac tgaagaagag aagaagattg tagtccttca ggcgacgggc
     2041 gtaaaattcc tggaaatgaa ctgcaaaccg ctcagcggag tggaggacta tgcatttcgg
     2101 gaaagcgcaa tccaggctgc gcatcgacgt cgcgccacgc atgtgcagct cctacctctg
     2161 gcaagcggaa tgagttcgac cggcggcttc gccacatcct atcagtcgtt ttcataccgc
     2221 gcatggtcct gtatttcgcc ctgacttctg ttcctggttg cccgcagggg tttcaagtgt
     2281 tcaatttcaa tgtcagcttc actttgcagg aaggacactc gctgactgta aagaagcgac
     2341 ccagccacgc cctgcaagcg ggacagcgcc agttcaccag atgaacccag accgccgcag
     2401 caagtgcggg aagaatggaa acgaacagaa tctccggttg tttaaacagg tctttctggt
     2461 aaattgcaaa tgcgaaaaat ggaaagacca gcggaacaaa ccccgcgaag agatttctcc
     2521 ttttgattct atcgaacgcg gcgcggattt cttcagggtt catctgactc aaggtccgcg
     2581 attgacggcc gcgggcaact gcattatgaa cttaaatatc gcacgaatta aatgcggacc
     2641 agagtatcac tcgcctacca tcgagggaat tgctccgctt tcgcgctgtt cgcggcgaat
     2701 ttccgcgcct caaaaaagat tgcgaggtgc cgcgagaaac taattatcgg agtcaaacat
     2761 gggcatgtct ggaaaggcgc tataaattga gtcgaaagcc agggccaatc gcatatccat
     2821 gaaacatttc attcgcctaa cattgctgtt gtatttcgta tctgctttgc cagccgcgcc
     2881 tgtgcacatg actgatgaag aagtgccggt atcaggaaca agtcttcaga tcatcgaaga
     2941 caaactggct gctctggacc tgaccaactt gccggagggc ctctggaaat cctcccacaa
     3001 cggagacttt ggagccggct tcacagaagc agtctactgg ctgaagtttt cggctgtgaa
     3061 tatgcgaccg gaggagaatt ggacacttca tattgagaac gcactcgtag acgagcttga
     3121 gatctggaaa acgggcggcg aatcggcgca catccaaacc ggaaccaaaa tcccttacaa
     3181 agaccgagag cttgcaggcc ggctcttcca tttgccggtc aaaatcaaga aaggtgaaac
     3241 tgcaaatttt ctcattcgcg ccaaaatgcg ggcgtcaatg aatcttccga tccgtcttcg
     3301 cacggacagg gtgactgctt cgtggaacaa ccgggagaat ttaatactcg ggcttttcca
     3361 cggcgccgtt ctcatcatga tcgtttacca tgcattcata tggttgtcgg tgagagatag
     3421 gatctatgcc tacttcattc tagtattgct cttcgggtcc actttcatgg ctgcgcaaaa
     3481 cggcctcgca tttcagtatc tctggcctgg catcggcgaa agaggcgttc agataaatct
     3541 cacgttcgga gtgctgacgc tgctcacgag cagtctcttc attagagaaa tgctcaatct
     3601 acgtgcacgc tggcttccgg gttttttcgc ggccaacgga ctcatcattt tgagcgcaat
     3661 ctgtcttgct gcagtctgga tcgtatcata caaaattgcc gcttcgacca tgactacctt
     3721 gaccttgatg caagccatgg tggccatgag tgtggggctg cgcttcgcgc tccggcgcga
     3781 caggattgca gcctggtttg cctctgccta tttcgttgtc atcgccagct ctgatctttt
     3841 tgcgctccga agtgtcggcg tgattccacc atcccaatgg atcactcatt cactcatgat
     3901 tggcattctc atgcagaaca ttctactatc attgtctctg gccgagcgga tcaatgtgct
     3961 gcacctcgag gccgaagtct caaagcaagc cagtgaactc aagactaagt tcatggcaac
     4021 tatgagccac gaaattcgca ctccgatgca cggtgtgatt ggactgacga acctcttgct
     4081 cgatacacag ctcgacgcag aacaacggat gctcgcaacg caaacatcgc gaagcgctca
     4141 agcattgctc gagatcatca atgacgttct agatttttcg aagatcgaag ctggcagact
     4201 tgaactgcat ccagagacta cccggatcac ggatcctgtc gcctccgtcg tcaacctcat
     4261 gcagcctctc gcacagaaga agggcctgca catacgcacg agcctcagtc gcgaaattcc
     4321 accatttgtg agcgtcgacg ctggacggct ccggcagatt ttattgaatc ttgtggcgaa
     4381 cgcagtgaag ttcacctctg aaggcgaaat cagtctgacc ggaggttacg agcagggaaa
     4441 tatcttcttc agcgttcgtg acacggggat tgggattgat tcggaatact tgccgcactt
     4501 gttcgaccca ttctaccagg tagattcgcg gctgacccga caatttcagg gcaccggcct
     4561 gggtctggcc atcaccaaac atcttgtgac cctcatggga ggcacaatcc gagtggaaag
     4621 cgaaccaaac gtgggctcag tttttgaatt ccgtctgcct gcgcctgcgg tggctgcgcc
     4681 aacagaaatc agaaaaccag atgaatctat ggttggtaat cttcgcgttc tcgtcgctga
     4741 cgacgacgaa gtgggcagac tcgtaggcgg acagtatctc cggaagctgg gctgcactgt
     4801 ggatcttgca gaagacgggc cagccgtgct caggtgcatg cagagtcaaa agtacgctgc
     4861 catatttctg gacatgcaaa tgccgcatct tgacggtccc gatactgcgc gcgagatccg
     4921 aaaactatac ggggatacgc catttctaat cgccttgac
//
LOCUS       DAORFF010000260         4953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9591_length_4953_cov_4.356268, whole genome shotgun sequence.
ACCESSION   DAORFF010000260 DAORFF010000000
VERSION     DAORFF010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9591_length_4953_cov_4.356268"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PKK76_11100"
     CDS             complement(<1..401)
                     /locus_tag="PKK76_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNL69066.1"
                     /translation="MNAEIIDTHCHLNMVQQAGISIEEAFANAKAAGVTSAVLIAVDE
                     ESARYHADLVQKKSTPGLQLLWTAGLHPEGADNVSGLQTLFAFVRESRDRLDFLGIGE
                     TGLDYYHSTEFVNNQKESFRAHLELSVELQLP"
     gene            complement(391..885)
                     /locus_tag="PKK76_11105"
     CDS             complement(391..885)
                     /locus_tag="PKK76_11105"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNL69067.1"
                     /translation="MKIEKKGVLFVCHGNICRSPAAEGVFQALIRKEKVTDRIFADSA
                     GTSDEHEGDLPHPVTRRVAITYGIDLDHKSRPFRKSDFKDFELILLMDSWNFRNVASL
                     ASSDKDMARLRMYRSFDPESTKLGRVPEVPDPYYGGIEGFEQVHAIFERTSPHILQWI
                     LSER"
     gene            949..1980
                     /locus_tag="PKK76_11110"
     CDS             949..1980
                     /locus_tag="PKK76_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNL69068.1"
                     /translation="MRRLLLPVFLNAALWPQIIQASSEPILFIFDASGSMSEPFQGVT
                     RMKAARFMLKEQITHLPLGTRVGLVAYGNGIPGCDSHRLYAPIARSSTGTIVREIDKM
                     QAAGETPIAATLRQVGKSILAKEKNARIILISDGKESCGGDPAAEAALLRARGFNVFV
                     IGLGVDDATAVDLRKIAGAGGGEYFHVHTNTEFIRAIELSTDTSSSKTDPDKDITSPI
                     TPPAVVSPAGPPVSSNVEMPDKDGIYLRGVRTSNLPDGRIKVEIDYSFKWIPRGDYML
                     TMQIFPGRRSQVPDGAASAVHYDSDHGSGTMVIELDPGDASGDSPIIVGELWDIGEAP
                     VRVSRAVAP"
     gene            1929..>4953
                     /locus_tag="PKK76_11115"
     CDS             1929..>4953
                     /locus_tag="PKK76_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69069.1"
                     /translation="MGYRRSPSQSQSRSCALILFILSIFFAASRVEAVPHADWSDLEA
                     PEAARVYGSEKEEKYPTNLFFIEREAWEGHHSWHAFWFAGQKDYPTFSSTRFIPFYYH
                     LWSKGDNRERTWIAPPFYFNQIDNDTSLTVTPLTLFDDSESRYLHMYGWFYMRRKESD
                     VTHTAVIPFVLTGNGPGTFYATVIPFFYYRSNESPEQKSSALFTPLYAHWSASGKENS
                     FFSISPLHFYNRSESSTSTGFPVIPMLMYRSVSGDTVHSNYLSIIDMETKENAGLRRL
                     LVLPLFYHYADDITWFIPFYFKGRDYSLIGPVWRSKSGEDETSLHVFPLFLSGGTKDT
                     AYSYSLLHFYSRDAAGTAFGFPILPFLMYRNSENGTVHSNYLSLFDMETNEKTGLRRL
                     VVLPLFYHFADDFTWFIPFYFSGKDYSLIGPVWRSNSGAEDSNLHVFPFFFSGTSKES
                     AYSYSPFHFYSRSGTDTAWGFPILPILAYKNQEGDRVHRNLLSFIDWSTNAKGLERLW
                     VLPLYFQGSDYLVAAPFYFSWEDGSTKRKNVLGFLDWRSGTQGLERLWVLPFVYYWPN
                     DALWVLPVFYRGSDYLAVAPFYFHTEDKVAKSSTSFGPVYYWYDSEKEHQRLIGNVWW
                     SSNGPSSSLIVAPVYFSWFEKPDPKGLAYSKEVYNPLFANVATYSSGESLEFEDTFWA
                     PIIPLYFHKATQDVDYRWALLASWSFDRTYGDLHHLAIAPFYFWKAGKKSGYLVAAPF
                     FIRPNGPESPQGVSFGITHYHSWTETSDFLVWGPYYHDYTPEAQYKHVLPVYWSWHTQ
                     QSTGQILFPLFVDYEDKTKSAHVNLSGYSSTKQAGIVGSSVGTKDGRYYLDTEVSWLY
                     NAFSISARISTPALDSKKDTKKEDSRKPVGDVAIREPTLRDVSTPTLREMADQEQKDT
                     KKAPHLAKTVNAGKEDSYYYWGVTVLYGLLSYQHADSMRHFRMLPFYWFSWDTNSNDK
                     VYVVPGLFLNYASADLEYFAIFPGFLPVYGRQRQGK"
BASE COUNT         1145 a         1219 c         1224 g         1365 t
ORIGIN      
        1 ggcagctgca attccacgga aagttccagg tgagcgcgaa agctttcttt ttgattgttc
       61 acgaattctg tggaatgata gtagtccagg cccgtctctc ctatccccag gaaatccagg
      121 cgatctctac tttcgcgcac aaaggcgaat aacgtctgca acccggatac attgtctgcg
      181 ccctcaggat ggaggccggc agtccataga agctgtaggc cgggtgtaga tttcttctgg
      241 actagatccg cgtgatagcg cgcagactct tcatccactg cgatgagcac ggcgcttgtc
      301 acacctgctg ctttcgcatt tgcaaacgct tcctcaatgg aaatgcctgc ctgttgtacc
      361 atattgagat ggcagtgtgt atcaatgatt tcagcgttca cttagaatcc attgaagaat
      421 gtgagggctg gtgcgttcga aaatggcgtg cacctgttca aacccctcta ttccaccgta
      481 atagggatcg ggcacttccg gtactctgcc cagtttcgtg gactctggat caaacgatct
      541 gtacattctc agtcgtgcca tatctttgtc cgagcttgcc agggatgcga cgtttctgaa
      601 attccagctg tccatgagaa ggatgagttc aaagtctttg aagtcgcttt tgcgaaaggg
      661 cctggattta tggtccagat caattccgta ggtgatggca acccgacgcg tcaccggatg
      721 aggtagatcc ccttcgtgtt catctgatgt tccagccgaa tcggcgaaga tccggtccgt
      781 tactttttcc tttcgaatca gagcctgaaa cactccttcg gctgccggag agcggcagat
      841 atttccgtga cagacaaaca atacgccttt tttttctatt ttcatagtgt ggatgcggct
      901 aaaggttttt tccaaccgga cgaccttgtg tacgctcgga gaaaccagat gcgccgactg
      961 ttattgcctg tcttcctaaa cgcggctctc tggccgcaga ttattcaggc ctcctccgag
     1021 ccaatccttt tcatcttcga cgcgtcgggg tccatgtcag agccgtttca gggtgtgacc
     1081 agaatgaagg ctgcccggtt catgctcaaa gaacaaatca ctcacttgcc cctcggaacc
     1141 agggtggggt tggtcgccta cggaaatgga attccgggtt gcgattcgca tcgactgtat
     1201 gctcccatcg ccagatcgag caccggcacg attgttcgcg aaatcgacaa gatgcaggca
     1261 gccggcgaaa cgccgattgc tgcgactttg cggcaggttg gaaagtcgat cctggccaaa
     1321 gagaagaacg ctcgcatcat tttgatttcc gatgggaagg aatcttgcgg tggggatccg
     1381 gctgcggaag cagcattgct tcgagcgcgc ggattcaatg tatttgtaat tggcctgggc
     1441 gtggacgatg caactgcggt ggatctgaga aagatagcgg gggcgggcgg gggcgaatac
     1501 tttcacgtac atacaaacac tgaattcatt cgtgctattg agttgagcac ggatacttct
     1561 tcctcgaaaa cggatcctga taaagacatc acatcaccca taactccgcc agctgttgtc
     1621 agtcctgcag ggccacctgt gtcgtctaac gtggagatgc cagacaaaga tggcatctat
     1681 ttgcgcggag ttcgaacttc caacctgcca gatggacgaa taaaagttga gattgattat
     1741 agtttcaaat ggattcctcg cggtgattac atgctcacca tgcaaatttt tcctggaagg
     1801 cgatctcagg ttccggatgg agccgcgtcc gcagtccact atgattcaga tcacgggtct
     1861 ggaacaatgg tgattgaact tgaccctggt gacgcatcag gtgactctcc catcattgtt
     1921 ggagagcttt gggatatagg agaagcccca gtcagagtca gtcgcgcagt tgcgccctga
     1981 ttcttttcat actgtctatt tttttcgcag cgtcgcgcgt tgaggcggtt cctcacgcag
     2041 attggtctga cctggaagca cctgaagcag ctcgcgtcta cgggagcgag aaggaagaaa
     2101 agtatccaac caatcttttc ttcatagaga gagaagcctg ggaagggcat cattcatggc
     2161 atgcattctg gtttgcgggg cagaaggatt acccgacttt cagttctaca cgtttcattc
     2221 ctttttatta tcatctctgg tccaaaggtg acaatagaga aagaacctgg attgctccac
     2281 ctttctactt caatcagatc gacaatgata cttctcttac tgtaactcca ctcacgttat
     2341 tcgacgattc ggagtctcgt taccttcaca tgtacggctg gttctacatg aggagaaagg
     2401 aaagtgatgt cactcacact gcagtgatcc cgttcgttct tacgggcaat ggtcctggta
     2461 ctttttacgc gactgtgatt ccctttttct attatagaag taacgaatcg cctgagcaaa
     2521 aatcgtccgc tttgtttact ccgctctatg cacactggag tgcatccggt aaggaaaatt
     2581 cattcttctc tatatctccg ttgcatttct acaatcgcag cgaatcgagc acttccacag
     2641 ggtttcctgt gattccaatg ctgatgtatc gaagtgtgtc aggcgatacg gttcacagca
     2701 actacctttc catcattgac atggaaacaa aagagaatgc cgggttgagg cgcctcctgg
     2761 ttttgcctct gttctatcat tacgcagatg atataacctg gttcattcca ttctatttca
     2821 agggcagaga ctattctctc attggtcctg tctggcgatc taagtcgggt gaagatgaga
     2881 catccttgca cgtgtttcct ttgtttctct caggagggac gaaagacact gcatattcct
     2941 attctttgct acatttctat tcgcgcgatg cggcaggcac ggcctttggt tttccaatcc
     3001 tgcctttcct gatgtatcgc aattccgaaa acggaactgt tcacagcaat tacctgtctc
     3061 tctttgatat ggaaacaaat gaaaagacgg gactgagaag gctcgttgtc ttgcctcttt
     3121 tctatcattt cgcagacgat ttcacatggt ttataccgtt ttacttcagc ggaaaggatt
     3181 actctctcat tggtccagta tggagatcaa actccggcgc ggaggattcc aatctgcatg
     3241 tgtttccgtt cttcttttcc gggacttcaa aggaatctgc gtattcgtat tcgccttttc
     3301 atttttatag cagatctgga acggataccg cgtggggatt tcctatcctg cccattctcg
     3361 cttacaagaa tcaagaagga gacagagttc acaggaactt gctttccttc atagactggt
     3421 ctacaaacgc gaaagggttg gagagactct gggtattgcc tctctatttt cagggatctg
     3481 attatctggt ggctgctccc ttctatttct cctgggaaga cggaagtacg aagagaaaaa
     3541 atgtactcgg gtttttggac tggagatccg gaacgcaggg gttagagcgg ttgtgggttt
     3601 tgcccttcgt gtactattgg cccaatgacg ctctatgggt tcttcccgtc ttctatcgtg
     3661 gttcagatta tcttgcagtt gcgcccttct attttcacac ggaggataaa gtcgcaaaat
     3721 cgagcacgag ctttggacct gtgtactact ggtatgattc agagaaggag caccagcgtc
     3781 tgatcggaaa cgtctggtgg tcgtctaacg ggcccagttc ttctctgatt gtcgcgccag
     3841 tctacttctc ctggtttgaa aagccggatc caaagggtct tgcatattct aaagaagtat
     3901 acaatccact tttcgcgaat gtggcgacgt actcgagcgg agagagtctg gagtttgagg
     3961 atacattctg ggctccgatt atcccgctct attttcataa ggcaacgcag gatgtggatt
     4021 atcgctgggc tctccttgca agttggagtt ttgatcgcac atacggggat ctacatcatc
     4081 ttgcgattgc tccattctat ttctggaagg cgggcaagaa gtcggggtat ctggtggccg
     4141 ctcccttttt cattcgaccc aatgggcccg aatcgcccca gggcgtctct tttggaatca
     4201 ctcactacca cagctggacg gagacttcag actttctggt ctggggccct tactatcatg
     4261 actacactcc ggaagcccaa tacaaacacg tccttcctgt ttactggtca tggcatacgc
     4321 agcagagtac cggtcagata ctatttcccc ttttcgtaga ctacgaagac aaaactaaat
     4381 ctgctcacgt gaacctctcc ggatactctt ccactaagca ggcgggcatc gttggatcat
     4441 ctgtcggcac aaaagacgga cgctactatc tggatacaga agtgtcatgg ctttacaatg
     4501 ccttcagcat ttctgcgcgc atttctacac cggctctcga ttcaaaaaag gatacgaaga
     4561 aagaagattc aagaaaaccg gtaggcgatg ttgcgattcg tgaacctact ctgcgcgacg
     4621 tcagcactcc gacgctgcgc gaaatggcgg atcaggagca gaaggacacg aagaaggctc
     4681 ctcatcttgc aaagactgtg aatgcaggca aagaggacag ttactactac tggggtgtca
     4741 ccgtgctcta cggattgctg tcctatcaac atgcggattc tatgcggcat tttcgcatgc
     4801 ttcctttcta ttggttttcc tgggacacga attcaaacga taaagtgtac gtcgtgcccg
     4861 gccttttctt gaactatgcg tccgcagacc ttgaatactt tgccattttt cccggttttc
     4921 tacctgtcta cggtcggcag aggcagggca aat
//
LOCUS       DAORFF010000261         4945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9615_length_4945_cov_3.620245, whole genome shotgun sequence.
ACCESSION   DAORFF010000261 DAORFF010000000
VERSION     DAORFF010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9615_length_4945_cov_3.620245"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PKK76_11120"
     CDS             complement(<1..215)
                     /locus_tag="PKK76_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69070.1"
                     /translation="MSEPKVFSSQALTIKVREDGPGFILEWTGKSNEREPGKFITPIL
                     TEIMEKAGQNPVVLDFGNLEYMNSSTIT"
     gene            complement(235..738)
                     /locus_tag="PKK76_11125"
     CDS             complement(235..738)
                     /locus_tag="PKK76_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL69071.1"
                     /translation="MPTIKNDTLTTEIKPDWGEIDHVRERTETFLKSCGFSDDIIHAL
                     QMVSSELVENAVKYGDFSAGGNQKITHSVDVRDGRITVEVKNPVKPSDEDHLDRLDSQ
                     IQWIRGFQNPFEAYVEKLKEVSAKMLAAGESGLGLVRIAYEGQSILDFYVDDHNILAV
                     SAVLEYK"
     gene            809..2140
                     /locus_tag="PKK76_11130"
     CDS             809..2140
                     /locus_tag="PKK76_11130"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL69072.1"
                     /translation="METSGKIFSREALANASREDLIEEIYKLNVVLDINQLFSSDLNP
                     DIVKNIVLLEAGRLTDAEYASIFLVTDAGDLEFAATTDPDRQQLEAIRIPMGKGIAGY
                     VAQSGEIVNTANIQKDTRFYRAVDELTGKVTRSYLCIPLRARDRIIGTIQVINKKTGG
                     HFAHADERILLTFAGQAAIAIETAILHEKAAEQERLAKENVRMSAELDVARRIQMSLL
                     PSAPTLRGLEIAATMVPADEVGGDYYDVIETDAGQWLLIGDVSGHGVESGLIMMMTQT
                     SIKSLVHKNQDANPASILSSVNRVLRENINRLGADRYMTISAIRINEDKLAYAGKHQD
                     LLIYRSSTDLIESLPTSGTWLGIADDIGEALVDEEIEFSSGDMLVLFTDGITEATNAQ
                     DEMFGPARLIQLVRDHRKSSPQAVVDQIIHAVRDFAKKPDDDYTLLVMRKL"
     gene            complement(2145..4613)
                     /gene="pheT"
                     /locus_tag="PKK76_11135"
     CDS             complement(2145..4613)
                     /gene="pheT"
                     /locus_tag="PKK76_11135"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNL69073.1"
                     /translation="MKLSYNWLNDYVDLKSIDIKDLINRLTLATCEVEEVTDAFPGIA
                     QLIIARIVSCEKHPQADRLQICQVDAGKFQGKIVCGAPNARPGILVPFAPVGATLPGK
                     DGKGMTIGKANIRGVESHGMLASAAEMGLDRIFAGEGLLELTDIPGALAGHSLTSIFP
                     ADLVITIDNKSITNRPDLWCHFGFAREIAAIYKKKIKFHPLDRKLTFKPSKLSKKILV
                     EKGSALAYFGAVCEGTRVLETPVWMKLRLGAVGQKSINNIVDASNYAMLELAQPNHTF
                     DLNTLKQNTVTVALSDSKSPDFKTLDGESRKLPPGTVLIFDGDKKAGKSPLAVALGGI
                     MGGEHSAIQPDTGTIFIESATFPRELIRRTLKKIDLRTDSAMRFEKGQDPAKAKPTIY
                     RIAELLRETCPDLKLGPVTGQSVLPKKSKIKVTTSFLQARLGFAVTSKEISTTLTWLG
                     YDVKSDKTGKTFQITAPTYRSQYDITIPEDIVEELGRVHGYDHIAPVPPVVPVLARSL
                     DHARLLERRMKRLLADAGFSETLNYSFVKEEDNLLFGKQGLALLNPLAGLSHMRLSLI
                     PGVLHHLAANQDRYAEVRLFEFGRVYDPTAKSSAFLHSAGPAHEEKRICVAMITDKID
                     ASAQEESFAWFRETVREMAGASARQLDTALPAKKDSWGIASSVSSLFHPSCQVVLKCD
                     GEAVGFIGMLHPVLAERFGIRRTVLVAEISFDLIAGEKKRASYKPPSVYPDSVFEVTV
                     ILEEAETTRRPVDVVMNLGAPEIHSMELTTIYRGAPIAEGKKAASYKIRCRKADGTIT
                     AEEWRPIFERVIDGIEGAGMPLRR"
     gene            complement(4626..>4945)
                     /locus_tag="PKK76_11140"
     CDS             complement(4626..>4945)
                     /locus_tag="PKK76_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNL69074.1"
                     /translation="FEREVTVRLRPGYFPFVEPGFELDIQCLVCGGPGCPVCKHSGWV
                     ELLPCGMVHPNVLRQGGLNPEKWNGFAFGLGLSRLVMMRYGIEDIRHFMSGNLRFLEQ
                     FPA"
BASE COUNT         1260 a         1246 c         1204 g         1235 t
ORIGIN      
        1 gtgatggtgg acgaattcat atattccaga tttccgaaat cgagaaccac agggttctgc
       61 cccgccttct ccatgatttc agtgaggatg ggcgtgatga atttccctgg ctctctttcg
      121 ttgcttttcc ctgtccactc cagaatgaat ccgggaccat cttcgcgcac tttgatggtc
      181 agcgcctggc tgctaaaaac tttaggttca ctcatgaatc cagctcccca ctatctattt
      241 gtactcaagc accgcggata cggcgagtat gttgtgatca tccacgtaga agtccagaat
      301 cgactgacct tcgtatgcga ttcgcactag ccctagtccg ctctctcccg cggcgagcat
      361 cttggcggag acttctttga gtttttctac gtatgcttca aaaggatttt gaaaaccgcg
      421 gatccattga atctgggaat ccagacgatc gagatgatct tcatccgagg gcttgactgg
      481 attttttact tccacagtga ttcttccgtc gcgcacgtcg actgagtgag tgatcttctg
      541 atttccgccc gccgaaaaat ctccgtattt gacggcgttc tcaacgagct cactcgatac
      601 catctgcaaa gcatggatta tgtcgtccga aaacccgcag cttttgagga aggtctctgt
      661 acgctctcgc acgtgatcga tttcacccca gtcgggtttt atctcggtag tcaaagtgtc
      721 gttcttgatc gttggcatgt ttaatcgcct gagagcgccg ttatgcagtt gccaaccctt
      781 acgttttttt aaacaatgtc aatggatttt ggaaaccagc ggtaaaatat tttcgcgcga
      841 agctctcgcg aacgcctctc gcgaagattt gatcgaagag atatacaaac tcaacgtcgt
      901 gctggacatc aaccagctct tcagctcgga tttgaatccg gacatcgtga aaaacattgt
      961 tctactcgaa gcgggaagac tcacagatgc ggaatatgca tcgatctttc tggtaaccga
     1021 tgcaggagat ctggaattcg cagccacaac tgatccggat cgtcagcagc ttgaagccat
     1081 ccgcatccca atgggcaaag gcattgccgg ttatgtcgct cagtccggtg aaatagtcaa
     1141 tacagcgaac atacagaaag acactcgctt ctatcgtgca gtggatgaac taaccggaaa
     1201 agtaacgcgc tcctatctct gcattccact gcgcgcgcgc gataggatca ttggcacaat
     1261 tcaggtgatc aacaagaaga caggcggaca ttttgcgcac gcagatgaaa gaattctact
     1321 aacgtttgcg ggacaggctg ctatcgccat tgaaaccgcc atccttcacg aaaaagcagc
     1381 ggaacaagaa cgcctcgcaa aagaaaatgt aaggatgtca gcagagctcg atgtggcgcg
     1441 ccgcatccag atgtcactgc ttccgtccgc acccaccctt cgcgggctcg aaattgcagc
     1501 cacaatggtg ccagcagatg aagtgggtgg agattattac gacgtcattg aaacggacgc
     1561 ggggcaatgg ttgctgattg gagacgtatc ggggcacgga gtagagtctg gactgatcat
     1621 gatgatgaca cagaccagca tcaaatccct ggttcacaaa aaccaggatg ccaatccagc
     1681 ttctatcctt tcgagcgtaa acagagttct acgcgaaaat attaacagac tgggcgcgga
     1741 tcgttatatg actatcagcg ccattcgaat caacgaagac aaactcgcat acgcaggcaa
     1801 acatcaggat cttttgattt atcgttcaag tacagatcta attgaatctc ttcccacgag
     1861 tggcacatgg cttggaatcg cagatgacat tggagaagca ctggtggatg aagaaataga
     1921 attctcgagc ggtgacatgc tggtgctgtt cacggatgga atcacagaag ccacaaacgc
     1981 gcaagatgaa atgtttggac ctgcaagact catccagctc gttcgtgatc acagaaaatc
     2041 aagtccgcag gcagttgtgg atcagatcat ccacgcagta agagatttcg caaaaaaacc
     2101 agacgatgat tacactcttc tggtaatgcg aaagctctga gtctttatct gcgaagaggc
     2161 attcctgcgc cttcaattcc gtcaatcacc ctttcaaaaa tgggacgcca ttcttccgca
     2221 gtgatggtac catctgcttt tctgcagcga attttgtaac tcgcagcctt ctttccttct
     2281 gcgatcggtg cgcctctgta aatcgtggtg agctccatag aatgaatttc aggtgcgccc
     2341 agattcatta caacatccac gggccttctg gttgtttctg cttcctctag tatgacagtg
     2401 acttcgaaaa cagagtcagg atatacggag ggtggtttgt aagaagctct cttcttttcg
     2461 ccagcgatta aatcaaaaga aatctcagct acaagcacgg tccggcgaat tccaaaccgt
     2521 tcggccagca caggatgaag cattccaatg aatcccactg cctccccatc gcatttcaaa
     2581 actacctggc aggaaggatg aaacagagac gaaacggaag aagcgattcc ccaggaatct
     2641 tttttagcgg gcaatgcagt gtccaattgg cgcgccgatg ctccggccat ttcgcgcaca
     2701 gtctcgcgaa accatgcaaa ggattcttcc tgcgcggatg catctatctt atccgtaatc
     2761 atggccacgc aaattcgctt ttcttcatgg gccggcccgg cagaatgcaa gaacgctgag
     2821 cttttggcgg tcgggtcata tactcgcccg aattcaaaga gtctcacttc cgcataacgg
     2881 tcctgattcg ccgccagatg atggagcact cctggaatca aactgagccg catgtgactg
     2941 aggcctgcga gaggattgag gagtgcaagg ccctgcttcc caaaaagcaa gttgtcttcc
     3001 tctttcacaa aagagtaatt caatgtttca ctgaaaccag cgtctgcaag gagcctcttc
     3061 attcgacgtt cgagaagccg cgcatgatcg agagatcgcg caagaacagg cacgacaggc
     3121 ggcacaggtg ctatatgatc atagccgtgc acccttccca attcttccac gatatcttcc
     3181 gggatggtga tgtcgtactg agagcgatac gtgggcgctg tgatctgaaa agtctttcca
     3241 gtcttgtccg atttcacatc atacccgagc catgtgagag ttgttgaaat ctcttttgaa
     3301 gtgactgcaa atccaagtct agcctgaaga aaggaagtgg tcactttgat cttgctcttt
     3361 ttgggaagga cactctgtcc ggtaacaggg cccagtttca aatcgggaca ggtttcgcgc
     3421 agtagttctg cgattctgta tatagtaggc tttgctttcg ccgggtcctg gcccttctca
     3481 aatcgcatgg cagaatctgt gcgaagatca attttcttga gagtgcgacg gatgagctct
     3541 cgcggaaaag tagcagattc tatgaagatg gttcctgtgt cgggttgaat cgctgaatgt
     3601 tcccctccca tgattccacc aagagccact gcgagaggtg actttcctgc tttcttatca
     3661 ccgtcaaaga tgagaactgt gccgggcgga agtttgcgcg attctccatc gagtgttttg
     3721 aagtcgggtg attttgaatc ggaaagtgca acagtaaccg tgttctgttt gagagtgttt
     3781 agatcgaatg tgtggtttgg ctgtgcgagt tcaagcatcg catagttcga agcgtctaca
     3841 atgttgttga ttgatttttg tccgaccgct ccaagtcgaa gcttcatcca taccggagtt
     3901 tcaaggacgc gtgtgccttc gcacacggcc ccaaaataag cgagagcaga tcccttctcc
     3961 accaggattt tcttcgaaag cttagaaggc ttgaaggtga gttttcgatc cagtggatga
     4021 aacttgatct tcttcttata gatggctgcg atttcgcgag caaatccaaa atggcaccac
     4081 agatccgggc gattcgtaat ggatttgttg tcgatggtga tgacaaggtc cgccggaaaa
     4141 atagaagtaa gcgaatgccc ggcaagagcc cctggaatgt cagtcagttc aaggaggcct
     4201 tctcctgcaa aaatccgatc cagtcccatt tcggccgcac ttgcaagcat cccgtgcgac
     4261 tccactccgc ggatgtttgc ttttccaatg gtcattcctt ttccgtcttt tccaggaaga
     4321 gtagcaccca caggagcaaa cggaaccaga attcccggac gggcgttggg cgctccgcat
     4381 acaattttgc cctgaaattt tccggcatca acctggcaga tctgaagacg gtctgcctgc
     4441 gggtgcttct cgcaacttac aatgcgagcg atgatgagct gagcgattcc agggaatgca
     4501 tccgtcacct cttccacttc gcatgtagca agtgttaggc gattgatcag atccttgata
     4561 tcaatggatt taagatcgac gtagtcattg agccagttat atgaaagttt cattgtgtta
     4621 ctcgattagg ctggaaattg ttccaggaag cgtagattgc cggacataaa gtgacgaata
     4681 tcctcgattc cgtatcgcat catcacaagc cgcgaaagcc ccagaccaaa agcaaaaccg
     4741 ttccatttct caggattgag cccaccctga cgaagaacgt ttggatgcac cataccacat
     4801 ggaagaagct ccacccagcc gctgtgtttg cacacaggac aaccaggtcc gccacagacc
     4861 aggcactgaa tgtccagctc gaagcccggt tccacaaacg gaaagtatcc aggacgcaag
     4921 cgcactgtga cttcgcgctc aaaga
//
LOCUS       DAORFF010000262         4936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9632_length_4936_cov_4.342143, whole genome shotgun sequence.
ACCESSION   DAORFF010000262 DAORFF010000000
VERSION     DAORFF010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9632_length_4936_cov_4.342143"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PKK76_11145"
     CDS             <1..262
                     /locus_tag="PKK76_11145"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HNL69075.1"
                     /translation="GVLEGNGGDLRVGLPLQSISDGKDILHEPLRLSVFIEAPRDAIE
                     DIVKRHETVRQLLDHEWLHLLQIDPSDGQVWRRLPGGDYTVV"
     gene            361..663
                     /locus_tag="PKK76_11150"
     CDS             361..663
                     /locus_tag="PKK76_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNL69076.1"
                     /translation="MKAAASKRELDKPEWTFLTNHAHVLLCLHRQPEMRMRDVAMLVG
                     ITERAVQAIVRDLEKVKCLRKEKKGRRNSYEIRTEIALRHPLENRKTVGDLLKAVK"
     gene            712..1545
                     /locus_tag="PKK76_11155"
     CDS             712..1545
                     /locus_tag="PKK76_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004789175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNL69077.1"
                     /translation="MNSSWYGKSILIAGITDRSSLALAIARHLQQLGVQLICTGLGET
                     SHHANLSEKSAQFLRKNYEDFTDTVAQELGPDVQTAVLDVTLDGSVAELAEHLRRKGT
                     KLDGIVHAVAMDKTIRGGFAKNLLDVTREEFAGAMDVSAYSLIALTRSLIECEVLAPG
                     ASVLSLSYLGASRVMTHPYKNIGVAKSALERITLEMAQELGLRGIRVNAIRFSPYAVS
                     KAGAAIPGLAEAVASCDSLSPLGNARPEDLARAAAFLLSPDLRITGQILHVDGGYSIG
                     G"
     gene            1711..1980
                     /locus_tag="PKK76_11160"
     CDS             1711..1980
                     /locus_tag="PKK76_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNL69078.1"
                     /translation="MKLFVGNLPFSITEDALHDEFAAQGSVISTKIITDRDTGRSRGF
                     GFVEMASLKDGQAAISGLNGREMDGRQLAVSQAKPQENRGGRDRY"
     gene            complement(2032..2919)
                     /locus_tag="PKK76_11165"
     CDS             complement(2032..2919)
                     /locus_tag="PKK76_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69079.1"
                     /translation="MRFSFQYTAITCDADLDSTRLRVKQGLRTFEISIHEIAHIYFHL
                     MPPGDFNELIVSRKNAAGKVKNFRFYSNANDPGLLSLTEELVKLKPGADLRGMDRKEA
                     FAHMKAVDTARYALFAVPIIVSLVVACMLSPMLIHGLDKGSSNLSAEDLVAGKMPESR
                     NVNLKGIALSDGMKETTTRKGTKTVRMFFPVVTTDWKEGDPVHILVETGDLTDDQLNA
                     VLASPEFKGVLRNVFWEGPSSKQLKFFQENYNTRFAEKVYLVELGQPSDLFMFLIVMG
                     ISVVMLVGVTIYMKKKMAA"
     gene            complement(2950..3378)
                     /locus_tag="PKK76_11170"
     CDS             complement(2950..3378)
                     /locus_tag="PKK76_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNL69080.1"
                     /translation="MVAVAICILISEPGFLCQLRDDVPGIRCPGQWGLFGGHIEEGED
                     ARTAVERELMEEICLDAKSFEYLGEFPDDGVIRHVFVHTVETLSGLILKEGWDMAVLT
                     REQLESGFAFSEKAGMIRPVAEPVRLIFRQFFQTRAQKTG"
     gene            3356..4024
                     /locus_tag="PKK76_11175"
     CDS             3356..4024
                     /locus_tag="PKK76_11175"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNL69081.1"
                     /translation="MQIATATTEQKGMTRCPGRLESTVLENWNHSFIGRVATTVVLIG
                     ITMPLYMWINHSLSPRFDLSISLDETIPFLPWTIFLYLSFYLMLLVAAGVCAAPEYGR
                     IQKSVLLANFLCYAGFFFFPSHYPRPLPMDAGGLRDFYGWLYANDAPGNTFPSIHAAA
                     SLCTGFSMRKIGWKIWGAGIAISILTVKQHFIVDLIGGAAVAALSIWIVRLYEGRRFH
                     ENRN"
     gene            4008..4904
                     /locus_tag="PKK76_11180"
     CDS             4008..4904
                     /locus_tag="PKK76_11180"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNL69082.1"
                     /translation="MRIETNIEPPGTLNLFLSSVIVAGWMGGQIIASSISGWTLVAFG
                     VAFSFLTLPLYSLMHECEHNIFHKNKWINDIAGTILSAFFPGPFSFLRGCHLGHHRRN
                     RTEAEMFEIIQPGDSAWKKTGFFYFMYLGGFWLSVPVSMIPLLLWPALLKSRMIQNTV
                     SASAMVNGVPRHFYLRIRLECVFVLVVHLALIWSLNLNPSTYLLILVAGGINWSSQQY
                     IQHAGSPLDVIHGAHNLKASRPYQKWILNFNWHLAHHQHPQVPWNMLPKFHDPSRDRP
                     PYLVSFLKFWKGPRRLEIRRDA"
BASE COUNT         1206 a         1259 c         1208 g         1263 t
ORIGIN      
        1 gggtgttctt gaaggcaacg ggggtgatct acgagtcgga cttcctctgc agtccataag
       61 tgacggaaag gacatcttgc atgaaccctt gcgtctgagc gtattcattg aggctccacg
      121 cgatgcgatc gaggatattg taaaacgcca tgaaacggta cggcagctac tcgatcacga
      181 atggctgcat ttgctgcaga tcgatccttc agatggacaa gtctggagac gcctgccagg
      241 cggtgactat actgtggttt aagatattcg tgaaatgaga ttcgcgtatt cgaggcgcga
      301 cccgcctttc gggtggatcg cgcttgacgt cgcccagggc atttgttacg ctcttcggca
      361 atgaaagccg cagcttcaaa acgggaattg gacaaaccag agtggacgtt tctaacgaac
      421 cacgcgcatg tcctcctctg ccttcaccgc cagccagaga tgcggatgag ggatgtagcc
      481 atgctcgtag gaattacaga gagggctgtt caggcgattg tcagggatct cgaaaaggtc
      541 aagtgtctgc gcaaagaaaa gaaagggcgc cgtaactcct atgaaataag aacggaaatt
      601 gcactgcggc atccgctcga aaaccgtaag acagtcggag atttgttaaa agctgtgaaa
      661 taattcacga aaaataatag gcgaaaattt cttcgtatat actatggtga aatgaattca
      721 tcatggtatg gcaaatcaat cctgatcgcg ggaatcacag accgctcttc cctcgccctg
      781 gcaattgccc ggcaccttca acagctcggg gtccagctga tctgcacagg acttggtgaa
      841 acatcacacc atgcaaacct ctctgagaag tcggcccagt ttctgagaaa gaactacgaa
      901 gacttcacgg acacggttgc gcaggagctc ggtccagatg tgcaaacggc cgtgctcgat
      961 gttacgcttg acgggtctgt cgcggaactt gccgagcacc tccgtcgcaa aggcaccaaa
     1021 ctcgacggga tcgttcatgc tgtagctatg gataaaacca tacgaggcgg attcgcgaag
     1081 aacctgctgg atgtgaccag agaagagttc gcaggcgcga tggacgtttc tgcttattcc
     1141 ttgattgcgc tgacgcgaag cttgatcgaa tgcgaagtgc tcgcgccggg tgcgtcagtc
     1201 ctttcgctca gctacctggg tgcaagtcgc gtaatgacgc atccatacaa gaacatcgga
     1261 gtggcaaagt ctgcactgga aagaatcaca ctggagatgg cacaagaact gggtttgcgc
     1321 ggaattcgcg tcaatgcaat tcggttttct ccctacgcag tgagcaaagc aggcgcagca
     1381 attccaggtc tcgcagaagc agttgcttcg tgcgacagtc tttcgcctct cggcaacgcg
     1441 aggccggaag atttggcgcg tgcggccgct tttctgctca gtcccgattt gcgcatcaca
     1501 ggacagatac tgcatgtgga cggtggatat tccatcggtg gttgagctat ttggaatttc
     1561 ttgttgacag tcgcgacctc aggattctat tggcttcaac accagccttt cttctctccc
     1621 cattctcgtc tttaagtcct cacaagtctt ttcagttcga tttgcagtga aatgaaaagt
     1681 aaaagtgtaa tttgatttaa ggaccatagt atgaaacttt ttgtaggaaa ccttcccttc
     1741 tccatcacag aggacgcact tcacgacgaa ttcgcggcgc aaggctccgt gatctcaacc
     1801 aagatcatca ctgatcgcga cacaggccgt tcacgcggtt tcggatttgt tgaaatggca
     1861 agcctcaaag acggtcaggc agcaatctcc ggcctcaacg gccgcgagat ggacggacgt
     1921 cagctcgcag taagccaggc aaaaccacag gaaaacagag gcggccgcga ccgttattga
     1981 ttctttttga attcggttct tccgaattca ctttgaggaa aggccgccta ttcaggcggc
     2041 cattttcttt ttcatgtaga tggttactcc aaccagcatc acaacgctga tccccatcac
     2101 aatcaagaac atgaacagat ccgaaggttg acccagctcc acaaggtaca ccttttctgc
     2161 gaatcgtgta ttgtaatttt cctgaaagaa cttgagttgt ttggaagaag gtccttccca
     2221 gaaaacattt cgcaagactc ctttgaactc tggcgaagca agaaccgcgt tgagttgatc
     2281 atccgtgagg tcccctgttt ccaccaggat atgcacagga tctccttctt tccaatccgt
     2341 agtcacaacc ggaaagaaca tgcgcaccgt ctttgttccc ttgcgagtcg ttgtttcctt
     2401 cattccatcc gaaagtgcaa ttcccttgag gtttacattt ctagattcgg gcatctttcc
     2461 cgccacgagg tcttctgctg atagattgga agatccttta tccagtccat gaatcagcat
     2521 cgggctgagc atgcacgcca cgactaaact cacgatgatc ggaaccgcga agagcgcata
     2581 acgggctgta tccaccgcct tcatgtgagc aaacgcttcc ttcctgtcca ttccgcgtaa
     2641 gtcggcacct ggtttgagct tcacgagctc ctctgtaaga gagagaagtc ctggatcatt
     2701 cgcgttggaa tagaagcgaa agtttttcac tttgccggca gcatttttcc tggaaacgat
     2761 gagttcatta aaatccccag gaggcatcaa atgaaagtaa atgtgcgcga tttcgtggat
     2821 cgaaatttca aaagtacgaa gcccttgttt tacgcgaagg cgagtagaat caaggtccgc
     2881 atcgcaggta atggcggtgt actgaaagga aaaacgcatg cgcagattct ccatctgcgc
     2941 cgcttgtcat caaccggttt tctgagccct ggtttgaaaa aactgacgaa agatcaggcg
     3001 aacaggctct gcaaccgggc gaatcatgcc cgccttttcg ctgaacgcga aacctgactc
     3061 tagttgctcc cgcgtgagca ctgccatgtc ccaaccttct tttagaatca agccggagag
     3121 agtttcaaca gtatgaacga atacatgacg aatcactcca tcgtccggaa attctcccag
     3181 atattcaaac gacttcgcat ccaggcaaat ttcttccatc aattcacgct caactgcagt
     3241 acgagcatct tcgccttctt cgatgtgtcc tccgaaaaga ccccattgac ctggacagcg
     3301 aattccaggc acgtcatcgc gcaactggca aaggaatcct ggctcggaaa taagaatgca
     3361 aatcgcgact gcaaccacag aacaaaaagg aatgaccagg tgtccgggtc gactagaatc
     3421 gactgtgctc gaaaactgga atcattcgtt tatcggccgc gttgccacca cagtggtgct
     3481 gattggcatt acaatgcctc tctatatgtg gatcaaccat tctctgtctc cgagatttga
     3541 cctgagcatt tctctggatg agacgattcc gtttctaccg tggactattt ttttgtatct
     3601 cagcttttat cttatgctac ttgttgcggc aggagtctgc gccgcgcctg agtatggtcg
     3661 aatccagaaa agtgttctgc tggcgaactt cctctgctac gcaggattct ttttttttcc
     3721 gtctcattat ccaagaccct tacccatgga cgcagggggc cttagagatt tctacggctg
     3781 gctttacgca aacgatgctc ctggaaacac atttccgagc attcacgcgg ccgcatccct
     3841 ttgcaccggt ttttccatgc ggaaaatagg ctggaagatc tggggagctg gaatcgctat
     3901 atctattttg acagtgaagc agcatttcat cgtggatcta attggcggtg cggcggtggc
     3961 cgccctgagc atctggattg tgcgattgta cgaaggccgg cgattccatg agaatagaaa
     4021 ctaacatcga accaccaggc acgctgaacc tatttctatc ctcggtgatt gtcgctggct
     4081 ggatgggcgg gcagataatt gcgtcttcca tttccggatg gacgttagtc gcttttggcg
     4141 tcgctttctc attcctcact ctacctctgt attctctcat gcatgaatgc gagcacaata
     4201 tcttccacaa aaacaaatgg atcaatgaca tcgccggaac gattctatcc gctttcttcc
     4261 ctggtccgtt ttcttttctg cgcggctgtc accttggcca tcacaggagg aaccgtacag
     4321 aagcggagat gtttgaaatc attcaacctg gtgactcagc ttggaagaaa actggtttct
     4381 tctattttat gtatctgggt ggtttctggc tttccgttcc tgtatctatg atcccgcttc
     4441 tgctctggcc cgcgcttctg aaaagcagaa tgattcaaaa tacagtaagc gcatctgcga
     4501 tggtaaacgg agttcccagg cacttctatc ttcgcattcg cctcgagtgt gtttttgtgt
     4561 tggttgttca tctggccttg atctggagtt tgaatctaaa tccctcgacg tacctgttga
     4621 ttctggttgc aggaggaatc aactggtcca gccagcagta cattcagcac gcaggctctc
     4681 ctcttgatgt gatccatggc gcgcacaact tgaaagcatc tcgcccgtat caaaaatgga
     4741 tcctgaattt taactggcac ctggcgcatc accagcaccc tcaggttccg tggaacatgc
     4801 taccgaagtt tcatgatcct tctcgcgatc gaccgcccta tttggtctct tttctaaaat
     4861 tctggaaagg ccccagaaga ctggagattc gtcgcgatgc gtgaagaaaa ctttctgggc
     4921 accaggttga atgatt
//
LOCUS       DAORFF010000263         4929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9653_length_4929_cov_3.520517, whole genome shotgun sequence.
ACCESSION   DAORFF010000263 DAORFF010000000
VERSION     DAORFF010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9653_length_4929_cov_3.520517"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..311
                     /locus_tag="PKK76_11185"
     CDS             69..311
                     /locus_tag="PKK76_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNL69083.1"
                     /translation="MGKTVTVRLDDKTYRMIRSAAEADHRTISNFMEVATLSHILEDN
                     FASDEEMGEILANKPLVKRLKASAAAAKKGKFRIVG"
     gene            301..576
                     /locus_tag="PKK76_11190"
     CDS             301..576
                     /locus_tag="PKK76_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016754088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNL69084.1"
                     /translation="MSAEFRIAETEFARTALASPKHKALAKKLNAFVYPQLRKNPFYG
                     PNIKKLKGELSDFWRYRVGKFRIFYSVEPGEGLVIIHDVQDRKDAYS"
     gene            complement(616..2115)
                     /locus_tag="PKK76_11195"
     CDS             complement(616..2115)
                     /locus_tag="PKK76_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNL69085.1"
                     /translation="MSKVAPPFSIAGARARENLPADEASMTDTKAKWVALIILCLGDL
                     MIVLDTTIVNVALPSIQRSLGFSEANLVWVVNAYMLTFGGFLLLGGRLGDLFGHRRLF
                     LVGISIFTIASLACGLAPSQLLLVAARAVQGAGGAIVSAVALSLIMDLFHEPEERAKA
                     MGFFGFVMAGGGSLGVLLGGVLTDSLSWRWVFLVNLPIGTLVSVLTLRLIEKTEGTHR
                     PLDLLGALLITASLMVLVYGIVGGNEMGWLSVQTLGFIGIGIVLFLAFLWLESRLTSP
                     LVPLGLFRLRNLSAASMIGMLWSAGMFAWFFLSALYMQHVLGYSAMKVGLAFLPSNVI
                     MAAFSLGLSARIVMRFGIQAPVSVGMVLVAVGLALFSRAPVNGDLFADVIPPMIVLGL
                     GAGLALNPLLLAAMTDVDENDSGLASGVVNTAFMMGGSVGLAVLASLAAYVSAGQSAA
                     TGKSVTLLAGYQWAFFVGALFALLASFISTARFRIPKSGAEGSKPVSLH"
     gene            2317..3813
                     /locus_tag="PKK76_11200"
     CDS             2317..3813
                     /locus_tag="PKK76_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69086.1"
                     /translation="MKTFAAAGLIAASLLVILCNLRFKQLYSIDSDTVDAFLMLEAAG
                     WHAHTPFHPGLLLGFSPYVLLKGMAEVSRFDAMRLNFAFYGCLAVLGCFAFAFVVTRD
                     SLRAAACTLLFSLTPAVQFVERFLDDNFPSYGFLFLVLTGLLSPGPPTTRLTVRVAIS
                     LLFFPPLLLFQPGFLPCLIVSGLGMIYMTFLFDWTWKDRLRTGALTALAFTLSACFTF
                     WALGAWLGVSVSALLHGMGSAIVDTSSNVASQTLPFVDRRGSFAFGLKHSIFAGSILH
                     SLDLNDLSYVLIGYDRMKGDFTALALGIAITIFVFFRGWKTKSNRDKFNAVTGASGLV
                     VLCAIPYAAANLVSQQERFDLLTVLLPFLLARVSFQGQSPWSFSNVWILSLLAAIFLA
                     GSITLLTPSSPESSWQRMKRILPEAPAASYLFAEGELRNHEDSVSLAMLGALPLSVIE
                     NPQGPEGVLAILRWMRPRYLISIDQARTLDLHNTFVSAEARNRLASRP"
     gene            complement(3822..>4929)
                     /locus_tag="PKK76_11205"
     CDS             complement(3822..>4929)
                     /locus_tag="PKK76_11205"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL69087.1"
                     /translation="AKKRDRHPATRYLVAMRFAVLLVLAFVSACRVSTDCAKGDPSCS
                     VGALLLSAQRSYRMKAFYVCRTTNLDTYLVNADIVTPAQSQAIGASTSWMRTDLDGKY
                     LYIQSSGNNVDWYSIDRDTGLIARVGTFAIALAGNYEPSPYYKNIMVASLGASKMRTF
                     SIGSDGSLTTLFINQDPGSTSPGKFVFHPTLPVVYTLETSTVRIQDLAANGALTFRTT
                     AAATATTTNIAIDPNGRYLAVANNAAPGIQLYTIDSTGGLSAPSSAAFTNISILAFDS
                     TGSFLMAGNTALPGVTTVNIPAMQSVQTFNTGTLPTTVIADRGGKYVLVGNPAPTLFS
                     LYQFTQTGGLPSVKYEFVPTGNVTGVGMYSPYGF"
BASE COUNT         1234 a         1271 c         1264 g         1160 t
ORIGIN      
        1 atggatttga tccgaatagt gttgactttt ctgaatacaa atgaatgcat tagaacagga
       61 tcgaatcaat gggaaaaaca gttaccgtac gcctcgatga taaaacatat cgcatgatca
      121 gatccgcggc agaagcagat cacagaacca tctccaattt catggaagtg gccacattat
      181 cccacatcct ggaagacaac ttcgcctctg acgaggaaat gggggagatt ctggcaaaca
      241 aaccgctggt gaagcggttg aaagcttccg cagctgcggc gaaaaaaggc aagttccgaa
      301 ttgtcggctg aattcaggat cgcagaaacg gagttcgcca gaacagccct cgcatctccg
      361 aaacacaaag cgcttgcaaa aaaactcaat gcattcgttt acccacagct tcgcaaaaat
      421 cctttctacg gtccgaatat taagaaattg aaaggagaac tttcggattt ctggagatac
      481 cgtgtgggga aatttcgcat attctattca gtggaaccag gagagggtct cgtgatcatt
      541 cacgacgttc aggatcgcaa ggatgcctat tcgtagtcgg cggctactca tcaactttcg
      601 ttgaatggca gccgttcagt gcagggacac aggttttgat ccttctgccc cggactttgg
      661 aatccgaaat cgcgccgtgc tgatgaagga agcaaggagt gcaaacagag cacccacaaa
      721 aaacgcccac tgataaccag caagcaaggt cacgcttttc cctgtggcgg cgctctgtcc
      781 ggcactcaca tacgctgcca gacttgcaag cacggccagc ccaacggaac cgcccatcat
      841 gaatgctgta ttgaccactc cggaagccag accagaatca ttttcgtcta cgtctgtcat
      901 ggcggcgagc aacagaggat tgagtgcgag accggctccc agtcccagaa caatcatggg
      961 tgggatcacg tccgcgaaca aatcaccgtt taccggagca cgcgaaaaga gcgccagacc
     1021 aacagctaca agcaccatgc ccacggacac tggagcctga attccaaatc gcattacaat
     1081 gcgggcagag agcccgagag aaaaggccgc catgataacg ttagacggca agaaagcaag
     1141 gcccactttc attgcggaat atccgagcac atgctgcata taaagcgcgg agagaaaaaa
     1201 ccaggcaaac atgcccgcag accatagcat tccaatcata ctcgcggcag aaagatttct
     1261 taatctaaaa agacccagag gaacgagagg tgacgttagg cgcgattcga gccagaggaa
     1321 agccagaaac aaaacgattc cgatcccaat gaacccgaga gtctgaacgg atagccagcc
     1381 catttcattg ccgcctacga taccgtagac tagaaccatg agagaagcag taatcaagag
     1441 agcgcccagg agatcgagag gccggtgagt tccctctgtt ttctcaatca ggcgtagtgt
     1501 aagaacactg accaatgtgc caattggcaa attcacgaga aacacccagc gccagctaag
     1561 cgaatctgtg agcacaccac ccaggagaac acccaggctg ccgccgccgg ccattacaaa
     1621 tccaaaaaag cccatggcct tcgcgcgttc ttccggctca tgaaacagat ccataattaa
     1681 agaaagcgct acagcagaga cgattgcacc tccggctccc tggactgcgc gggctgctac
     1741 gagcaaaagc tgcgagggag caaggccgca cgcgagggac gcaatcgtaa agatagaaat
     1801 tccgacgaga aacaaacgtc tgtgtccgaa aagatcgcca agtcgtccgc ccagaagtag
     1861 aaatcctccg aacgtcaaca tgtaagcatt cactacccag acaagatttg cttctgaaaa
     1921 acccaggctt ctttgtatag aaggaagtgc tacatttaca atcgttgtgt ccaggacgat
     1981 catcagatct cccagacata gaatgatcag ggcgacccat tttgcttttg tgtctgtcat
     2041 gctcgcttca tccgcaggca ggttttcgcg agcgcgagca cccgcaatcg aaaatggcgg
     2101 agcaacttta gacaaatcgt cgcacgaatg aacgaagcac ctaacctcct atgccgaagc
     2161 gtcttagaat cgcataacgg taagcgatcc gtccacgcgc agatttttcc gagtcccgga
     2221 accgtgtgca aagagtagcc cgggaaggaa tggacttgac tgattccttt ttcttttgac
     2281 tttaaatgta tggagacacc cggcggggca aatcgagtga agaccttcgc tgccgcagga
     2341 ttgatagcag cgagtcttct ggtcattctg tgcaatttaa gatttaagca actgtactcg
     2401 atcgactcag atactgtaga cgcatttctg atgcttgaag ctgcgggctg gcatgcgcat
     2461 acaccctttc atcctggatt gctgctcgga ttttcgcctt acgtgctgct aaaggggatg
     2521 gccgaggttt cccggttcga tgcgatgcgt ttgaattttg ctttctatgg atgcctcgct
     2581 gttctcgggt gtttcgcatt tgcattcgta gtgactcgcg attctttgcg cgccgccgcc
     2641 tgcaccctgt tgttttctct cacgccggcg gtccaatttg tagaacgttt tctcgacgac
     2701 aattttccgt catacggatt cttgtttttg gttttaactg gtttactttc tcctggaccg
     2761 cccaccacgc ggttaacagt ccgagttgcg atctccctgt tgttttttcc tccgctgctg
     2821 ttgtttcaac ctgggtttct accttgcttg attgtgagcg gccttggtat gatctacatg
     2881 acgtttttgt ttgattggac ctggaaggat cgcttgcgaa cgggtgcgct cactgctctt
     2941 gcttttactc tctctgcatg cttcacattc tgggctctgg gagcctggct tggcgtctcg
     3001 gttagcgcgt tgcttcatgg gatgggttct gcgattgtag atacgtcaag caatgtagct
     3061 tcgcagacgc ttccttttgt ggatcggcgt gggtcttttg cctttggctt gaagcacagc
     3121 atctttgccg gttcaatctt acattctctc gacttaaacg atctctccta tgtgttgatt
     3181 ggttacgatc gcatgaaggg cgatttcact gcactggcgc tggggattgc aatcaccatc
     3241 ttcgtcttct ttagaggatg gaagaccaaa agcaatagag ataagttcaa tgcagtgacg
     3301 ggtgcgagcg gccttgtggt actctgtgcg attccgtacg ccgccgccaa tcttgtgagc
     3361 cagcaggaaa ggtttgacct gctcacagtt ctcttgccgt ttctgctcgc acgcgtgtct
     3421 ttccaggggc agtctccgtg gagtttctcg aacgtttgga tcttaagtct gcttgcagct
     3481 atattcctcg ccgggtccat tacattgctt acaccttcaa gccctgagtc gtcgtggcaa
     3541 cggatgaaac gaattttgcc ggaggcaccg gcagcttctt acttgtttgc ggagggagag
     3601 ttgcgcaacc acgaggattc agtatccctg gcgatgctgg gagcactgcc actctccgtg
     3661 atcgagaatc cacaagggcc tgagggcgtt ctggcaatcc tcagatggat gaggccgcgc
     3721 tacttgattt ccatagacca ggcaagaacg cttgatttgc acaatacctt cgtaagcgcc
     3781 gaggcacgaa atcgcctggc ttcgcgtccg tagcgcgcca gctagaatcc gtagggactg
     3841 tacattccga ccccggtgac gttgcctgtt ggaacgaact catatttcac agacggcaat
     3901 ccgccagttt gcgtaaactg gtatagagag aacaaagtcg gcgcgggatt tccaacgagt
     3961 acatactttc cgccgcggtc tgcgatgacg gtggtgggca gagtgcctgt attgaaagtc
     4021 tgtacagatt gcatggcagg aatgttcacc gtggttacac ccggcagtgc ggtgttccct
     4081 gccataagaa agctccccgt cgaatcaaac gcaagtatgc tgatgtttgt aaatgcggcg
     4141 ctcgaaggag cactcaggcc tcctgtagaa tcaattgtat agagctgaat tccgggtgcg
     4201 gcgttgttgg caacggcgag atacctccca ttaggatcga tggcgatgtt cgtggtagtc
     4261 gcagttgcag ccgcagttgt gcgaaaagta agcgcaccgt ttgccgccag gtcttgaatt
     4321 cgaactgtgc ttgtctcaag agtgtacaca acgggaagcg ttggatgaaa cacgaatttt
     4381 ccaggcgagg tgctgcccgg gtcctggttg atgaaaagag tcgtcagact tccatctgag
     4441 cctatgctga acgtacgcat cttggatgca cccagagacg ccaccatgat attcttgtag
     4501 taagggctgg gttcataatt accggccaga gcaattgcaa acgttcccac gcgcgcgatc
     4561 aagcctgtat ctcgatcaat ggagtaccag tctacgttgt ttccagaaga ttgaatgtac
     4621 aaatacttcc cgtctagatc tgttcgcatc cagcttgttg aagctccaat cgcttgcgac
     4681 tgagcgggcg taacaatatc agcgttcaca agatatgtgt ccagattcgt agtgcgacag
     4741 acgtaaaagg ccttcatccg ataagaccgc tgggcagaaa gtaacaacgc ccccacgctg
     4801 caggaaggat cccctttcgc gcaatcagta gacacacggc acgcactgac gaaagccagg
     4861 accagaagca cggcaaaacg catggcaaca agatatcgcg tggccgggtg cctgtctctc
     4921 tttttagct
//
LOCUS       DAORFF010000264         4929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9654_length_4929_cov_2.712351, whole genome shotgun sequence.
ACCESSION   DAORFF010000264 DAORFF010000000
VERSION     DAORFF010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9654_length_4929_cov_2.712351"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PKK76_11210"
     CDS             <1..851
                     /locus_tag="PKK76_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69088.1"
                     /translation="DRLDAYELAFHLYFREERKENAIDLLSWACRSLEDGRYISCYDL
                     GQLLLREGRKDDAARAFAESYRISPNGPALSALYDLWATGTGPTPPPAPALVVISKTV
                     ERFCRAGDRQAAMIALKSSPADIPRELFAQPFFQECLFNQPEYAPFIAGRTPRPVPID
                     EIYRQRAAVHPLHAVLDVAPYLKAEYLAFESNHPVNVSWKLFLNAVRARNQVAAESAL
                     ANFYAALEKQRVPGGAADSQILAMKRAAGTLIAGDIFFASIRGSARIQKMVRDTLPGS
                     GIFASK"
     gene            886..1047
                     /locus_tag="PKK76_11215"
     CDS             886..1047
                     /locus_tag="PKK76_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69089.1"
                     /translation="MKRPHNRIEKITNNPKVVSLALYRIKKGLKKEGFELITDEQGKL
                     KLVMRKNKS"
     gene            1116..1601
                     /locus_tag="PKK76_11220"
     CDS             1116..1601
                     /locus_tag="PKK76_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNL69090.1"
                     /translation="MKIEKHKVVSIDYTLKDDQGNIIDSSRGGEPLAYIQGVGNLIPG
                     LESALEGGSKGDRLSVSVPPERGYGVLEDHLIHVLRRDQFEGVEELEVGMQFHAHADN
                     GAVQTVVVTKIDGDNVTIDGNHPLAGKNLHFDVAVVEVRDATAEELEHGHVHGPGGHH
                     H"
     gene            complement(1741..2100)
                     /locus_tag="PKK76_11225"
     CDS             complement(1741..2100)
                     /locus_tag="PKK76_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69091.1"
                     /translation="MNDQETAQRMIDAVKSVNLDAIEGARDAVNRSILEILLPEFEKL
                     TDDYQRAAFAMLICDLMDPITLPVMRKVLSLPDTEDIFNIARATALDHLAGKFGTAPD
                     WTRKEDLNRAVLEALWS"
     gene            complement(2124..2789)
                     /locus_tag="PKK76_11230"
     CDS             complement(2124..2789)
                     /locus_tag="PKK76_11230"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNL69092.1"
                     /translation="MAKTEISRSKRASASPRKATVRSYHHGDLRSAVLDEADRLLEKH
                     GIENLSLRDVAQNLGVSHAAPYRHFPRKLDLLLALAEKGFSELGDAMEKAFTAKSPEE
                     RFQSSGLLYVKLAVAHPVRTQLMFSKTIQCDDAPDSLQQAGQRAFQGLVSIIEDGQKA
                     GEFKTNSDAMTLAVSAWSMVHGIAGLISGGQLAEAAGKEDGFVRTILSQLYSGVRKEK
                     RGR"
     gene            2931..4490
                     /locus_tag="PKK76_11235"
     CDS             2931..4490
                     /locus_tag="PKK76_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69093.1"
                     /translation="MRRIFFLLFTCLCLAQVSAEIHPPQSEKTPDKSGVTSKVTFSPD
                     LYSLEFIQQEDGSFKPSTENVTITFERKDPGTFCTKIKEVSASVWGPATEYVTVQDEQ
                     RGMWVANGNRMEFRGKTYKTSTGKSPGFCNFDGARIVIPAFEILSHMGTSSMNSSDRL
                     KSADVCGRYDENPVTHVPDFLFPRPVYIDTGFNSENAMDSYTRESRRILVSTRCRRAK
                     PEGAREVILRAAGKYLGVDPSRGFEMRSDRSSAGDTERFWLRSGASENWIRLQSTVGG
                     KYACQNDQNGQVYAAYNFDGKGKGCMDPRLEIVSPGRVRITGNVWVTTGSQTAAQKRY
                     WVPLDGLVKGSANATPETVFEILDANTRKPIAFSSVAGAKNSFSMGLADGSLSGAKLT
                     WKLVRVSDGMELNMPAPNQGSLIIEDFVGTEYRMDFFVSYQGKEYRPGDKPTVRQNEM
                     QPAVTGIVNANVQGSDEKPAAPPENRTFSLLEGDRKSHWYKVSFKEYLNVDTNKIRPN
                     KPDLLKPGPNR"
     gene            4610..>4929
                     /locus_tag="PKK76_11240"
     CDS             4610..>4929
                     /locus_tag="PKK76_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69094.1"
                     /translation="MKRFSDLLRMNPPLVLTVVANLGLFCVLLPLAFLDTRLVTGQPV
                     WIKPLKFAISVSLYSGTLMWLLGYVKKQVLARRVAWIVAVTLLIEMILIVLQAARGVA
                     SHFNV"
BASE COUNT         1199 a         1223 c         1293 g         1214 t
ORIGIN      
        1 atgatcgttt agacgcatac gagctcgcat ttcacctata ttttcgagaa gaaaggaaag
       61 aaaacgcaat agatttgctg tcctgggctt gcaggagcct ggaggatggg cgctatatca
      121 gctgttacga cctgggccag ctcttgctcc gagaaggcag aaaagacgat gcggcgcgcg
      181 catttgcgga atcgtacaga atttcgccga acggacccgc tctgtctgcc ctctacgatc
      241 tctgggcgac cggtacaggt ccaactccgc ctccagctcc cgcactcgtt gtgatttcca
      301 agacagtgga gcgattttgc cgggccgggg atagacaggc agccatgatc gcccttaaat
      361 cctccccggc ggacataccc agggagcttt tcgcacagcc attttttcag gagtgcttgt
      421 tcaaccagcc agagtatgct cctttcatcg ctggtcgaac tccacggcca gtgccgatcg
      481 atgagatcta tcgccagaga gcggctgttc atcctctaca tgcagtgctc gatgttgctc
      541 cttatctgaa agcggaatat cttgcgtttg aatcgaacca tccagtgaac gtttcgtgga
      601 aattgttttt gaatgcggtg cgcgcaagaa accaggttgc ggcggaaagc gcgctggcaa
      661 acttttatgc tgcgctcgaa aaacagcggg tgccaggcgg cgccgccgat tcacaaattc
      721 tcgccatgaa gcgtgcggcc ggaactttaa tagcaggaga catttttttt gcgtctattc
      781 gcggttcagc gcggattcag aaaatggtga gggatactct gccaggctcc ggaatcttcg
      841 cgagcaagtg aattttcacc atgcgatgat ccgatcatgg agtagatgaa gcgcccccat
      901 aatcgcatcg agaaaatcac gaacaatccc aaagtagtga gtcttgctct gtacaggatc
      961 aagaaggggc tgaagaaaga aggctttgag ctgatcacag acgagcaggg aaaattgaag
     1021 ctcgtcatgc gtaagaataa gagctaaaaa acagttgccc ggcgtaaatc gagtcctaat
     1081 ttgattggat aacggctcga acgtccggag gaactgtgaa aatcgaaaaa cataaagtcg
     1141 tcagcattga ctacacgctg aaagacgatc aaggcaacat cattgattca tcgcgcggag
     1201 gagaacctct cgcatacatc cagggagtgg gcaatttgat tcctgggctt gaatccgctc
     1261 tcgagggcgg atcaaaggga gatcgtctca gtgttagcgt tcctccggaa agaggatatg
     1321 gagtcctcga agatcaccta attcatgttc tgcgacgcga tcaatttgaa ggcgtagaag
     1381 agctagaagt tggaatgcag tttcacgcgc atgcggacaa cggcgcggtt cagacagttg
     1441 tagtcacaaa gatagacggg gacaatgtaa cgatagacgg caatcatcca cttgctggaa
     1501 agaatcttca ttttgatgtg gctgtcgtgg aagtgcggga tgctactgca gaagaactcg
     1561 aacacgggca cgttcacggt ccaggcgggc accatcacta gaagctagcg tgcttgaatg
     1621 aatccgcgag agcggattca ggtctgcttg caagaagtag cgtggtactg cgcaatcaca
     1681 tcggttctgt catccagaat ccgcaggagg gtacgttata cgatttttct caaccttccg
     1741 ttaggaccag agcgcttcca gaactgccct gttcagatct tccttgcgtg tccaatcggg
     1801 cgcggtacca aatttcccgg ctaaatggtc cagcgctgtc gcccgcgcaa tgttgaaaat
     1861 gtcctcggta tccggcagac tcagtacttt gcgcatcacg ggtagagtga tcggatccat
     1921 caggtcacaa attagcatag caaaggccgc gcgctggtag tcgtccgtga gcttctcgaa
     1981 ttcaggcagc aatatttcga ggatgcttct gttcaccgca tcccgcgcgc cctctatcgc
     2041 atccagattc acgcttttca cagcatcgat cattctttga gcagtttcct ggtcgttcat
     2101 atcgcgtatt tcgtataacc gtctcagcgc cctctctttt cttttcgcac gcctgaataa
     2161 agctgcgata gaatggttcg cacaaatccg tcctctttgc ccgccgcctc cgccagctgt
     2221 ccgccggaaa tcaaaccggc gattccgtga accatgctcc acgcggaaac agccagagtc
     2281 atcgcatcag aatttgtttt gaactctccg gctttttgtc cgtcctctat gatcgaaacc
     2341 agtccctgga acgctctctg gccggcttgc tgcaaagaat cgggagcatc atcgcactga
     2401 attgtttttg aaaacatcaa ctgggttcgc acaggatgag cgactgcaag cttcacgtac
     2461 agcagccccg acgattgaaa gcgttcttct ggactttttg ccgtgaacgc tttttccatt
     2521 gcgtcgccga gttcactgaa tcccttttct gcgagagcaa gaagtagatc gagtttgcgt
     2581 gggaaatgtc tatagggtgc cgcatgactc actccgagat tctgagcaac gtcgcgaagg
     2641 ctcagattct cgattccgtg tttctcaagc aatctgtctg cctcgtccag tactgccgag
     2701 cggaggtcgc catgatgata cgatcttact gttgctttgc gggggctggc agaagcgcgc
     2761 ttagagcgcg aaatttccgt ttttgccatg tatacagtgt ctacattttc cgaatgtacg
     2821 tcaagcagaa cgcggctgct tcgcccgcct gcgtcgcact tcgggcaata tccaggttct
     2881 aaaatttttt taatattgat caaatcgcta ccgtactgca tctaaaccgc atgcgaagaa
     2941 tatttttcct tctgtttaca tgcttgtgcc tggcgcaagt ttctgcggag atccatcctc
     3001 cccagagcga gaagactccc gataagagcg gagtaacttc aaaagttacc ttttcgcctg
     3061 atctttattc tctggaattc atccagcaag aggacggttc gttcaaacct tctacagaga
     3121 atgtaaccat cacttttgag cgcaaagatc ccggaacgtt ttgtacaaag ataaaggaag
     3181 tcagcgcatc cgtttggggt ccggccacgg aatacgtcac tgttcaggac gaacagcggg
     3241 gcatgtgggt tgccaatgga aatcgaatgg agttcagagg caagacatac aaaacatcca
     3301 ctggcaagtc accagggttc tgcaactttg atggcgccag aatagtcatt cccgcgtttg
     3361 aaattctcag ccacatggga acaagttcca tgaattcgtc cgatcgtttg aagagtgcgg
     3421 atgtgtgcgg tagatacgac gaaaatccag tcacgcatgt gccggatttc ttgttcccgc
     3481 gacctgtcta cattgacaca ggattcaact cagaaaacgc gatggattct tatacccgcg
     3541 agtcacggcg aattctggtg tccactcggt gcagacgtgc gaagcctgaa ggtgcgcgtg
     3601 aagtgattct gcgcgccgca ggaaagtacc tgggcgtgga cccttcgcgc ggttttgaaa
     3661 tgcgttctga tcgttcgagt gcaggcgaca cagagcgttt ctggctgcga agcggtgctt
     3721 ccgaaaattg gattcgcctg cagtccaccg taggcggaaa gtatgcatgc caaaatgacc
     3781 agaatggtca ggtgtatgca gcctacaatt ttgacgggaa aggcaagggt tgcatggacc
     3841 cgcgacttga aattgtaagc cctggtagag tgcgaatcac cggaaatgta tgggtgacca
     3901 ccggaagtca aaccgccgca cagaaacgat actgggtgcc gttagacggc cttgtgaaag
     3961 ggtctgcgaa cgcaactcca gagacagtct ttgaaatcct ggatgcgaat acgcgcaaac
     4021 caatcgcttt ttcgagcgtt gcgggtgcga agaattcttt ttcaatgggc cttgcggatg
     4081 gttctctgtc tggagcgaag ctcacctgga aattggttcg cgtttcagat ggaatggaat
     4141 tgaacatgcc cgctcccaac cagggaagtc ttattataga ggatttcgtt ggaacggagt
     4201 atcggatgga cttctttgtc tcttaccagg gcaaagagta cagacctgga gacaagccca
     4261 ccgtccgcca gaatgaaatg cagcccgcgg ttacagggat cgtgaatgca aatgttcagg
     4321 gcagtgatga gaagccagcc gcaccgcccg aaaacaggac cttctccctc ctcgagggag
     4381 accgtaaatc acactggtac aaggtttctt tcaaggaata tttgaatgtg gacacgaaca
     4441 agattcggcc gaacaaaccc gatctattga aaccgggccc aaatcgttag gtttcgcaag
     4501 tctgtggaat gtagacactg actacttttt ttgttgaccc gccctccggg tcatgtatac
     4561 agtgtctaca ttcaataacg gtcgctgaat tttgctgccg ggagactata tgaaaagatt
     4621 ttctgatcta ttgcgaatga atccgcctct cgttcttacg gtcgtggcca atcttggttt
     4681 gttctgtgtg cttctacctc tcgcattcct ggacaccagg ttggtcacgg ggcagccggt
     4741 gtggatcaag ccactgaagt tcgctatttc tgtttcactc tattcaggaa cgcttatgtg
     4801 gcttcttgga tatgtgaaga agcaagtact ggccagacgc gttgcctgga ttgtagctgt
     4861 gactcttttg atcgagatga tcctgattgt tttgcaggct gcgcgaggag tggccagtca
     4921 cttcaatgt
//
LOCUS       DAORFF010000265         4927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9659_length_4927_cov_3.854885, whole genome shotgun sequence.
ACCESSION   DAORFF010000265 DAORFF010000000
VERSION     DAORFF010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9659_length_4927_cov_3.854885"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="PKK76_11245"
     CDS             <1..1342
                     /locus_tag="PKK76_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL69095.1"
                     /translation="VAWLGDADYEKIGEEQGALPGLLYKARQQAFHDAFLGKAAAEND
                     FNSDVWGTFLSAAVPIKDKDGTVIAVFGVDYDAGSEANKVAQLLKVCIAVVVASLVLS
                     IIVALLLARILSKPVQALREGAERVRERDFSTHVSVKTRDELGLLADTFNSMVTEIRD
                     YAKEMVTLNDAYYRFVPKEFLQQLERTSIVEVKLGDQVQREMTVLFSDIRSFTTLSEQ
                     LTPKKNFNLLNQYLNVVSPTIRQNQGFIDKYIGDAVMAIFPDSPQHAVEAGIAMLKAL
                     REYNELFRIGMDPIRIGVGIHSGLLMLGTVGEERRMEGTVISDAVNLASRLEGLTKKF
                     GSGILISEQVYQAVQGANKFHTRYLGRVRVKGKKESVKVYEVMDGDSEQLMQKKLQTH
                     DEMEKGIDLFTAGKFRESIGHFETVLASNPDDKAARAYMERADRVVKKPTVQIA"
     gene            complement(1323..2843)
                     /locus_tag="PKK76_11250"
     CDS             complement(1323..2843)
                     /locus_tag="PKK76_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNL69096.1"
                     /translation="MSKIKQHILTGTALAAAFLLQLFSSGNWMSASAPWVGLPIWLLL
                     FRYGNRAIVSVAAVVFLGASLVAWRGMIDDLFLRFAVIGGIGSLTLLLFLIDRILVRR
                     LVESRGVCVASFVFPTALTAMELILTRLSPYGSWSILGYTQSSNLPLIQMAAVTGVYG
                     VSFLIGWVSSVLCFLVRYAFAPEESSVSLGFHRVRLLVVSCFVLSGSIFLMGTVRMTL
                     SQETPSVRVAGITVDMSHSRGDVLHSVFDGSISKFDLKTYDAELSKIRDELFEATARE
                     AHAGARLVLWPETNGAILAGQNDSLLDRAARTAKELRIYLLITPTVIHPGKNRMDLRA
                     ALFGPDGSLLFDYDKTTVVPGDPNIPGPGKLPFAMTSFGKVSAAICFDGDFPGLIRQA
                     GQGRADLLLLPSSDWREIDPVHSRMTVFRAIENGSNLFRQTNLGLSLAADYNGRVLGE
                     TDHFRASDRSLVVQMPTSGVWTPYSAAGDMFSWLAAIGFLILLTLAMKANVRRSEP"
     gene            complement(2908..4020)
                     /locus_tag="PKK76_11255"
     CDS             complement(2908..4020)
                     /locus_tag="PKK76_11255"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNL69097.1"
                     /translation="MFIPAFWLSFSGPTFMMTLLAFGAGLSFLLAISRAIRRTPESYA
                     AAALFSCASIVLLHLYLELTARLSAFPHFVYWNVPALFCTGPLTHFYLLMLMRSPGER
                     KPGWIHFLPAIVSFFILIPFFIQSGETKTAFVTVPGFDASNPYQFIGMALISLGVLVN
                     LFYLLFLFARIFRLRSVPGNWDRSALSPFLIVLGATTVAILAMTLAQFFFKEIMHVAA
                     ASLGALIVILFLFGETSVRDDFAREIKRAKYAQSRIAGLDVNAVLARLDQIMREERAY
                     LDEDVSLARLADDLQIRSQQLSEILNARIGLGFRDYINGFRLEEAARLLIEEPRRSIL
                     SILFASGFNSKSSFHKLFQARFHCSPAEYRQKQTEV"
     gene            complement(4068..4184)
                     /gene="rrf"
                     /locus_tag="PKK76_11260"
     rRNA            complement(4068..4184)
                     /gene="rrf"
                     /locus_tag="PKK76_11260"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(4217..>4927)
                     /locus_tag="PKK76_11265"
     CDS             complement(4217..>4927)
                     /locus_tag="PKK76_11265"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL69098.1"
                     /translation="CLFLMHHLYSTSMEVEGKLAEERKRSDELLLNILPAKIAEELKE
                     SGAAEPVHFDSVTIVFTDFQGFTRSAESMPARELVRELDEYFRAFDEICERFGLERLK
                     TIGDSFMCAAGVPEISSDHAIVALRAALAMRDYSLSRTKGPRWGVRIGVHSGPVVAGV
                     IGRKKFAYDLWGDTVNTASRMESSGEPGAVNCSEATKSLAGEVFTFESRGKIEAKGKA
                     PMEMFFCLGEGKNFHSLS"
BASE COUNT         1423 a         1267 c         1209 g         1028 t
ORIGIN      
        1 ggttgcctgg cttggtgatg cggactatga aaaaattgga gaagagcaag gtgctcttcc
       61 aggcctttta tacaaagcgc gccagcaggc cttccacgat gctttccttg gaaaggcagc
      121 ggcggaaaac gattttaact ccgacgtatg gggcacgttt ctatccgctg cagttcctat
      181 taaagataag gatggcacgg tcatcgcagt gttcggagta gactatgacg cagggagcga
      241 agcaaacaag gtagcccagc tactcaaagt ctgcatcgca gtggttgtag cttccctggt
      301 tctgtccatc atcgtggccc tcctccttgc acgcattctc tccaaaccgg tgcaggcgct
      361 ccgcgaaggt gcagagcgcg tgcgcgaaag agatttcagc acgcatgtat ccgttaagac
      421 cagagatgaa ctcggtctgc ttgcggacac gttcaatagc atggtaacag agattcgcga
      481 ttatgcgaag gagatggtga cactgaacga tgcttactat cgttttgtgc caaaggaatt
      541 ccttcagcag ctagaaagaa caagtatcgt ggaagtaaag ttaggcgatc aggttcagcg
      601 agaaatgaca gttctattct ccgatatccg gtccttcacc actctttctg agcaacttac
      661 accaaaaaaa aacttcaatc tgctcaatca gtacctcaat gtcgtgagcc cgactattcg
      721 tcaaaatcaa ggcttcattg ataagtacat cggagacgct gtcatggcga tcttcccgga
      781 tagcccacag catgcagtag aagcgggcat cgccatgctc aaggcacttc gcgaatacaa
      841 cgaactcttc cgaattggaa tggaccccat tcgcattggc gttggaattc atagcggact
      901 tttgatgctg ggaaccgtgg gcgaagaacg aagaatggaa ggcactgtga tttcagacgc
      961 agtgaatctg gcgtcgcgtc tggagggact gaccaagaaa tttggatccg gcatcctcat
     1021 tagcgaacag gtctaccagg ctgtgcaagg tgccaacaag ttccatacac gatacctggg
     1081 acgcgtacga gtgaagggaa agaaggaatc tgtaaaggtt tacgaagtca tggatgggga
     1141 ttcagagcag ttgatgcaga agaagctgca aacgcatgat gaaatggaaa agggaatcga
     1201 tctgtttact gcaggcaaat tccgcgaatc cattggccat ttcgaaaccg ttctggcatc
     1261 aaacccggac gacaaagcag cccgcgcgta catggagcgc gcggaccgcg tagtcaagaa
     1321 gcctacggtt cagatcgcct aacgtttgct ttcattgcga gcgtcagaag aataagaaat
     1381 ccgattgctg ccagccagga gaacatatct cctgcagcag aatacggtgt ccatacaccg
     1441 gacgttggca tttgaaccac gagagagcga tcgcttgcgc gaaagtgatc cgtttccccc
     1501 aggactctgc cgttgtaatc tgcggccagg gacaagccca gattcgtctg gcgaaaaaga
     1561 ttcgagccgt tttcaatcgc gcgaaacacc gtcatgcgag aatgcaccgg atcgatctcg
     1621 cgccagtccg aggaaggaag aagaagtaag tcggcacgtc cctgacctgc ctgacgaatc
     1681 aatccaggaa aatcgccatc gaaacaaatg gcggcagaaa cttttccaaa agaagtcatc
     1741 gcaaaaggaa gtttccctgg acctggaatg tttggatcac ccggcactac ggttgttttg
     1801 tcgtaatcaa agagaagaga accatccggc ccaaagagag cagcgcgaag atccatcctg
     1861 ttttttccag gatgaatcac agtgggcgtg atgagcaaat agatcctgag ttcttttgca
     1921 gtcctggccg cacgatccag tagagaatcg ttttgtccgg cgagaatcgc tccatttgtt
     1981 tcaggccaga gaaccaggcg cgcacctgca tgtgcttctc gtgccgttgc ttcaaacaac
     2041 tcatcgcgaa ttttggagag ttccgcatca tatgttttca agtcgaactt cgaaatggat
     2101 ccatcaaaca cagaatgcaa aacatctcct cgcgaatggc tcatgtcgac tgtgatgccg
     2161 gccacgcgaa ccgacggagt ctcctgcgac agagtcatgc gaacagtgcc catgaggaaa
     2221 atggaaccag acaaaacgaa acaagaaaca acaagaaggc gaacgcgatg gaacccgagc
     2281 gagacggacg attcttctgg cgcgaacgcg tatctaacaa ggaaacaaag aacagaagaa
     2341 acccagccga tcaaaaaaga aacgccgtaa actcccgtga ctgctgccat ttgaatcaaa
     2401 ggaagattcg aactctgtgt gtaccccaga atgctccagc ttccgtacgg actcaaacga
     2461 gtgaggatca attccatcgc agtcaaagcg gttggaaata caaagctcgc gacacagact
     2521 ccgcgagact caaccagcct gcgaactaaa atccgatcga tcaaaaatag aagcaaagtc
     2581 aaagaaccaa tgccgcctat cacggcgaac ctcagaaata gatcgtctat cattccgcgc
     2641 cacgccacaa gagaagcccc tagaaaaacg actgcggcca cggacacgat cgcgcggttt
     2701 ccatatcgaa aaagaagaag ccagatggga aggcccaccc acggagcgct tgcggacatc
     2761 cagttgccgc tgctgaatag ttggagtaga aatgctgccg caagagcagt ccccgtcagt
     2821 atgtgttgtt ttattttgct catactccat tggaacacga aacaagagca aagtcgtccg
     2881 tcgggatacg gacggactca aaaatcatta cacctcagtt tgtttctggc gatattccgc
     2941 gggcgaacaa tggaagcgtg cctgaaagag cttgtgaaag gacgattttg aattgaaacc
     3001 ggacgcaaat agaatcgata gaatggaccg ccgcggctcc tcaatcaaga gtctcgcggc
     3061 ctcctccagc ctgaatccat tgatgtagtc gcgaaagcca agacctatcc ttgcattcag
     3121 aatctctgag agttgctgcg aacgaatctg cagatcgtct gcgaggcgcg ccagagacac
     3181 atcttcgtcc agatacgccc tttcctcgcg catgatttga tccagcctgg ccagaaccgc
     3241 attcacatcc agccccgcga ttctactttg cgcatatttt gcacgcttga tttcgcgggc
     3301 aaaatcatcg cgtacactgg tttcaccaaa caaaaaaaga attacaatca atgcgcccag
     3361 actcgccgcc gcgacgtgca tgatctcctt gaagaagaac tgcgccagag tcatggcaag
     3421 aatcgcaacg gtggtagctc ccagcacaat caagaacgga gaaagagcgg atctgtccca
     3481 gttccctgga acggacctga gcctgaagat tcgcgcaaac aaaaacaaaa gataaaacaa
     3541 attcacaagc acacccagac taatcaaagc catcccaatg aactggtacg gattgcttgc
     3601 atcaaatcca ggcacagtca caaacgctgt ttttgtctct ccactctgaa taaagaaagg
     3661 aatcaaaata aaaaacgaaa cgatggcggg taaaaaatga atccagccgg gcttgcgttc
     3721 tccaggagat cgcatcaaca tcaaaagata aaaatgagtc agaggcccgg tgcaaaaaag
     3781 agcaggcaca ttccagtaca cgaaatgggg aaaggcagaa agtcgcgcgg tcaactccag
     3841 atagagatgc agtaacacaa tgcttgcgca cgaaaacaat gcagccgccg cgtaagactc
     3901 cggagtgcgg cggatggcgc gagaaattgc caggagaaaa gaaagcccgg caccaaaggc
     3961 aagcaatgtc atcatgaaag tgggtccact gaaggagagc cagaaagcgg gaataaacaa
     4021 gaaaggctcc cgcaggagcc tttgtggaat gatctagatg attgcaatgt cggcaccgtc
     4081 ctactctccc atgaggcgaa cctcatagta ccattggcga tgaggggctt aacttctgtg
     4141 ttcggaatgg gaacaggtgt accaccctct ctattggcac cagacaatac caacataatt
     4201 gccaaccccc tgcgagtcaa ctcaaactgt gaaaattttt cccttctccc agacaaaaaa
     4261 acatctccat gggtgccttg cctttcgctt caatctttcc gcgcgattca aacgtgaaca
     4321 cttctcccgc cagcgacttt gtagcttccg aacaatttac tgcgcctggt tctccagaag
     4381 actccattct acttgctgtg tttacagtat ctccccagag atcgtaggcg aacttctttc
     4441 taccaatcac tcccgcaacc actggaccag aatgaactcc aattcgaact ccccagcgtg
     4501 ggccttttgt gcgcgaaaga gaatagtcgc gcatggcgag agctgcgcga agagcgacga
     4561 ttgcatgatc gctcgaaatt tcagggacac ctgcggcgca cataaagctg tctccgatcg
     4621 tcttgagtct ttcgagacca aagcgctcac atatttcatc aaacgcgcga aagtactcat
     4681 ctagttcgcg cacgagttcg cgagcaggca tgctctccgc agatcgggtg aaaccctgaa
     4741 aatcagtaaa tacaatggtc acggaatcaa aatgaactgg ctctgccgct ccggattctt
     4801 taagttcttc tgcgatcttc gcaggaagga tgttgagcaa tagttcgtcg gatctctttc
     4861 tttcctctgc gagtttgcct tccacctcca tactcgtgga gtacagatga tgcatcaaaa
     4921 aaagaca
//
LOCUS       DAORFF010000266         4920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9685_length_4920_cov_3.921274, whole genome shotgun sequence.
ACCESSION   DAORFF010000266 DAORFF010000000
VERSION     DAORFF010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9685_length_4920_cov_3.921274"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PKK76_11270"
     CDS             complement(<1..232)
                     /locus_tag="PKK76_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69099.1"
                     /translation="MRLIRVIKRRVFILIFLSTILFCMACLSGLKGPRNPEARPWTNE
                     EQKVSFMLGMVHLIEPGTLVPDECSDRVARFLH"
     gene            303..1871
                     /locus_tag="PKK76_11275"
     CDS             303..1871
                     /locus_tag="PKK76_11275"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNL69100.1"
                     /translation="MQKHILAIDLGTSGPKVAIVSTTGQVMASEIEETQLILSHGGGA
                     EQDPADWWSAIARATRRLMESRHVPVESLAGIGVTAQWSGTVCVDAAGKHLSHAVIWM
                     DSRGADYVKKLTRGLIQIQGYGLSRLLKFIRLTGGAPGHSGKDSIAHILYIQATNPSL
                     YADTYKFLEPKDFLNLKLTGKFAATFDSIALHWLTDNRNINEIAYHDGLVSMAGIDRA
                     KLPDLKRSTDIIGVISPQAAAELGLPENLPVIGGTPDIQSAAIGSGAVRDFEAHLYIG
                     TSSWLTCHVPFKKTDLLHNMATLPSAIPGRYFIANEQETAGYCLTYLRDKIFYAQDSL
                     GIQPAPKDAYVRMTEAAASVPPGSDSLIFTPWLYGERTPIEDHSVRGAFYNQSLSTTR
                     AHMIRAVLEGVAFNSKWLLGAVESFMKRRAESINVIGGGARSALWCQILADVFDRTIR
                     QVHDPVQANSRGVGLLAAAALGLTTFEEISAQIPVSATYRPNSENRAVYDSLFKEFKN
                     IYKQNKSIYGRLNP"
     gene            1868..3400
                     /locus_tag="PKK76_11280"
     CDS             1868..3400
                     /locus_tag="PKK76_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNL69101.1"
                     /translation="MIDPKTFAADHPALARFVEKTLLRFPVLDQWIRAIPSVRRQTEK
                     EYESLLGGMEESMKPYRKDFASYPELPKVGRKRSDILKDIQKMKAREESKWKSGYVSG
                     AVYHGDADHIDFLNKVYALQSQSNPLHFDLWPSINKYESEIISMTARMLGGTEDKSAD
                     QICGSMASGGTESILLAMKSYRDFARRERGIRRPELVVPVTAHAAFDKAAQYFKIKLV
                     RVPVGPDFRADVKLMEKAVTRNTIAMVGSAPTFPHGVIDPIRELSEIARKKGIGFHTD
                     ACLGGFVLPFARTLGYHVPPFDFRLPGVTSMSADTHKYGYAAKGTSVVLYRGEKLRSY
                     QYYTVTDWPGGLYFSPTFAGSRPGALIAAGWAAMLSMGEKGYLDATRKIMEAAHAIKS
                     EIRKIPDLYILGEPLWILAFASDTLDIYRVMEFMTHKGWNLNGLHKPACVHIAITLRH
                     TQPGVTKRFIKDLTDAVAHVRKHPEETGGMAPVYGMAATLPIRSVIGDLLKKYMDLLY
                     RV"
     gene            complement(3409..4134)
                     /locus_tag="PKK76_11285"
     CDS             complement(3409..4134)
                     /locus_tag="PKK76_11285"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HNL69102.1"
                     /translation="MTEHRPQLSRPLVFIGQNLAIALAYVLGARVGFSLAFLNSQVSP
                     IWPPEGIALAALLLLGRSAVPGVLIGAVVANYLESPKVETATLIGLGNTLGAILNAWI
                     VTRWVGANRILSSTSGLVRFLFGVIPGSALSALIGVTALWFFDIVKTPVYGNVLLTWF
                     SGELQGFLIAAPFLLTWAGFFQEKWDFRKLIEGVFLISLLAATTYLAFSQFGKIGEKI
                     DFRSVLIAVPRAFTWELGEKPDK"
     gene            complement(4141..>4920)
                     /locus_tag="PKK76_11290"
     CDS             complement(4141..>4920)
                     /locus_tag="PKK76_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69103.1"
                     /translation="QSRRQACPRFRPDASQAGLIRKQYMKLKYSPPTGFIKEMPILSK
                     YLLNPSPSTFADCHNSLDKRENALTGSLKKGSSLQSQEPLRQIQMRREIFLRNHVWAL
                     PSEDSYDFAARYLRAHDHRVESLHAFRGLFRLRIRKLWVVDEHESVESRMLVFAATMR
                     DMAALYGLLGRLADRSFLDQMADAIESVRPVTPAQAEGLKATLFKIAGQNLRNREALE
                     VLFLVDSPKSDFYRHKIEERDTILSDSELLEVFGPMYNPFP"
BASE COUNT         1219 a         1256 c         1267 g         1178 t
ORIGIN      
        1 aatggaggaa tcgtgccacg cgatcggaac attcgtctgg aaccagtgtg cctggctcta
       61 taagatgaac cattcccagc atgaaagaca ctttttgttc ttcattggtc catggccgtg
      121 cctctggatt tcgtggccct ttgagcccgg aaaggcaggc catgcaaaac aaaatagttg
      181 ataagaatat aagaatgaac actcggcgtt tgatcacgcg gatgagacgc acaggagagt
      241 gcgttgggtt ctcttttttt cgttgcgggg gaaggcggat cagtcgattt cgaaccatcc
      301 ttatgcagaa gcatattctt gcgattgatc tgggaacctc cgggccaaag gtcgccatcg
      361 tttctactac gggtcaggtg atggcctctg agatagaaga gacacaactc attctttccc
      421 atggcggtgg tgcagagcag gatcctgcag actggtggtc tgcgattgct cgtgcgacgc
      481 gccggctcat ggaatcgcgt catgtgcctg ttgaatctct ggccgggatt ggagtcacag
      541 cgcaatggtc tggcacagtt tgcgtggacg cggcgggcaa acatctttcg catgctgtga
      601 tctggatgga ttcgcgcggg gcggattatg taaagaagct aacgcgcggt ttgattcaaa
      661 ttcaaggcta cgggctttcg cgtcttttga aattcattcg tctgacgggc ggcgcaccag
      721 gacactcagg aaaagactcc atcgcgcata ttctgtacat ccaggctaca aatccttctt
      781 tgtatgcaga cacatataag tttcttgaac caaaggattt tctgaatcta aaactcaccg
      841 gcaagtttgc ggccacgttt gattccatcg ccttacactg gttaactgac aatcgcaata
      901 tcaatgaaat cgcctatcat gatgggctcg tctcaatggc cggcattgat agagcaaagc
      961 ttccagatct aaaacgatcc acagatatca ttggcgtaat ttctccacag gcagccgctg
     1021 aactcggcct acctgaaaac cttccggtga ttggtggaac gccagacatt caatccgcgg
     1081 ccatcgggtc tggtgctgtg cgcgatttcg aagcccatct ctacatagga acaagctcct
     1141 ggcttacctg ccatgtccct ttcaagaaga cggatcttct tcacaacatg gcaactcttc
     1201 cttccgcgat tccagggcgc tatttcatcg caaatgaaca ggaaactgcc ggctactgcc
     1261 ttacctactt gcgcgataag attttttacg ctcaggattc cctgggaatt cagccggccc
     1321 ccaaagatgc atatgtgcgt atgacggagg ctgccgcatc tgtgcctcct ggatcggaca
     1381 gcctcatttt tactccatgg ctgtacggtg agcgcacgcc aattgaagat cactctgtga
     1441 gaggagcgtt ttacaatcaa tcgctgagca cgactcgcgc tcatatgatc cgtgcagttc
     1501 tagaaggagt cgcgtttaat tcaaagtggc tcctgggtgc tgtggaatct ttcatgaagc
     1561 gcagagcgga gtctatcaat gtgattggtg gtggggcgcg ctccgctctg tggtgccaga
     1621 ttctcgcgga tgtatttgat cgaaccattc gtcaggtgca tgacccggtc caggcgaatt
     1681 cgcgcggagt ggggttactt gctgctgcgg ctcttggatt gactaccttt gaggaaattt
     1741 cggcacagat tcccgtttct gcaacgtaca gacccaactc ggagaaccgg gcagtctacg
     1801 attcactgtt taaagaattt aagaatattt ataaacaaaa taaatctatt tacgggcggc
     1861 tgaacccatg atcgatccaa agactttcgc tgctgaccat ccggctctgg ctcgatttgt
     1921 ggagaaaacg ctccttcgtt ttcctgtcct tgatcaatgg atcagggcca ttccatccgt
     1981 gcgtcgtcag actgagaaag aatacgaatc tctcctgggc ggcatggagg aatccatgaa
     2041 gccgtatcgc aaggacttcg cttcgtatcc tgagctgcca aaagtcgggc gcaaaagaag
     2101 cgacattctc aaagacattc agaagatgaa agcgcgcgaa gaatcaaaat ggaagagcgg
     2161 atacgtgagc ggagccgtgt atcacgggga tgcggatcac attgatttct taaacaaagt
     2221 gtacgctctt caatctcaat ccaatccact gcattttgat ctctggccca gtatcaataa
     2281 gtacgaatcc gaaatcatat ccatgaccgc gcgcatgctc ggcggcacgg aggataaatc
     2341 ggcggatcaa atttgcgggt ccatggcaag cggcggaacg gaaagcatct tgctcgccat
     2401 gaaatcgtat agagacttcg cgcgtcgcga gcgtggtatc aggcgtccgg aacttgtggt
     2461 tcctgtgaca gcgcatgctg cattcgataa ggcggctcag tatttcaaaa tcaagctcgt
     2521 gcgtgttccg gttggcccgg actttcgtgc agatgtgaag ctcatggaga aagcagttac
     2581 gcgaaacacg attgcaatgg ttggatccgc gccaactttt ccgcatggag tcattgatcc
     2641 cattcgggaa ctttcagaga tcgcgcgaaa gaagggaatt gggtttcaca cggatgcgtg
     2701 tctgggcgga tttgttctgc ctttcgcgcg cactctcgga tatcatgttc cgccgtttga
     2761 ctttcgcctt ccaggtgtga cttccatgtc cgcagatacg cataagtatg gttatgcggc
     2821 caaaggaacg tctgtcgttc tttatagggg agaaaaactc cgcagctatc agtactatac
     2881 ggtgacagac tggcccggcg gattgtattt ttcccccaca ttcgcaggca gtcgtcccgg
     2941 tgcattgatt gccgctggct gggccgcgat gctttctatg ggagaaaagg gatatctgga
     3001 tgcgacgcga aagatcatgg aggccgccca cgcaattaaa tcagagatca gaaaaatccc
     3061 ggacctgtat atactgggag aaccactctg gattcttgcg tttgcctccg acacacttga
     3121 tatttatcgc gtcatggagt tcatgacgca caaaggctgg aacctcaatg gccttcacaa
     3181 accagcttgc gttcacattg ccattacttt gcgccatacg cagcctggtg taaccaaacg
     3241 attcatcaag gatctaacag atgcagttgc gcacgtgcga aagcaccctg aagaaaccgg
     3301 cggaatggct cctgtttacg gaatggccgc aaccctcccg attcgcagtg tgatcggcga
     3361 tctgctaaag aagtatatgg atttgctata ccgagtttga tttgcggatt acttatccgg
     3421 tttttctccg agctcccagg tgaatgctct gggaactgca atcaacacgg atctgaaatc
     3481 tatcttctct ccaatttttc caaattgcga aaaagcaaga taggtggtgg ccgcaagcaa
     3541 gctaatgaga aaaacgcctt ctatcagttt gcgaaaatcc catttctcct gaaagaaacc
     3601 ggcccaggtc aaaagaaaag gagcagcgat caagaaaccc tgtagctctc cgctaaacca
     3661 ggtgagaaga acatttccat agaccggtgt cttcacaatg tcaaagaacc acagtgcagt
     3721 cacaccaatc aatgcgctga gcgcagagcc agggattaca ccaaagagaa atcgcacaag
     3781 ccctgacgtc gaagaaagga ttcgattggc gccgacccac ctggttacga tccaggcgtt
     3841 cagaatggcg cctaacgtgt tgcccagtcc aattaaagtg gcagtctcca cctttgggct
     3901 ttccagatag tttgccacaa cggcacctat cagaactccg ggtacggcgc tccttcccag
     3961 aagcaaaagc gcggccagag cgatcccttc gggaggccag atgggcgaaa cctgagaatt
     4021 caggaatgca agcgaaaacc ccacccgcgc acccagcacg tatgccaggg caatggctag
     4081 attctgacca atgaaaacca gtggccggga aagttgcggg cgatgctcgg tcatctcaga
     4141 tcaaggaaac ggattgtaca tcgggccaaa gacttccagc aattcgctgt cgctcagaat
     4201 ggtgtccctt tcctctattt tatgacgata gaaatcggac tttggcgagt cgactaaaaa
     4261 caaaacttcc agggcctctc tatttcttaa attctgccct gcgatcttga aaagcgtggc
     4321 cttgagccct tctgcctgcg ccggagtcac cggccgaacg gattcgattg cgtctgccat
     4381 ctgatccaga aaacttctgt cggccagtcg cccgagcaaa ccgtagagcg ccgccatgtc
     4441 acgcatcgtc gccgcgaaaa ccagcatccg cgattcaacg gattcatgct cgtctaccac
     4501 ccagagcttg cgaatccgca acctgaacag cccgcgaaac gcatgaagac tctcgactct
     4561 gtgatcgtgg gcccgaagat agcgcgccgc aaaatcatac gaatcctcag aaggaagcgc
     4621 ccacacatga ttgcgaagaa agatttcgcg cctcatttgt atctggcgaa ggggttcctg
     4681 agattggagt gagctcccct tcttgaggga tccagtcagt gcgttttctc tcttgtccag
     4741 ggagttgtgg caatccgcga aggttgaagg cgaagggttc agcagatatt tactcagaat
     4801 cggcatctct tttataaagc ccgtgggcgg agaatacttt agcttcatat actgttttcg
     4861 aatcaatcct gcctgcgatg cgtcaggccg aaagcgtggg caagcttggc ggcgtgattg
//
LOCUS       DAORFF010000267         4918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9691_length_4918_cov_3.396257, whole genome shotgun sequence.
ACCESSION   DAORFF010000267 DAORFF010000000
VERSION     DAORFF010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9691_length_4918_cov_3.396257"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1798
                     /gene="cfpA"
                     /locus_tag="PKK76_11295"
     CDS             <1..1798
                     /gene="cfpA"
                     /locus_tag="PKK76_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytoplasmic filament protein CfpA"
                     /protein_id="HNL69104.1"
                     /translation="NQSYVNMFNRYTVTVEDEMVKKVREFIDKEELRTLQRYTPREIV
                     EILDKVGGADKFNTGELEKSVVNMYGHLQGHIQRGMNDLENETNSILRQKTDVGAFVR
                     GENAYSIVKCSFKDSQFKPKTVTDIKLSINILDSELISPIFHYQTTVEHLLKDSISKH
                     ITELIDREIDKLKEELVDTGRAELSDGEIMFEKIKLVDKYTDDNVSDPNSRRYTILAK
                     KFLDKIEGLRAEIDQDDYDSMNMREQIKKIIDSENIRNRGFNTAVNTLTSILDTSKLG
                     YQVCDNKKNARECWVREYEDQDDARLPDERYAIRLVYLDQNQIRDLKRAYDARIAAFL
                     REGEAVWDVVHEVYDRTKKRFFGLSRVVKDFEDLARSIMPKAWVKERTTVNNDPDEIH
                     WDKLRFRDAPTTFVEKNNRTYSMEVKNVGEKIRYLRDRLQTMYGYQNPVERVVIDERL
                     NLLEQTFNKYIYEINPHHLQPGLILDVDITTTKRKMYMMKSMANVLNEFLSGVSQGFA
                     DAAFASFKRRRSTVRADIEMTFSQEEHKDSASSLAYKGQEPAAGSMYAAPSAPEPQRA
                     ASAGGESGVRGSADVTPKSYEDKLKEMGLKEL"
     gene            1903..3000
                     /locus_tag="PKK76_11300"
     CDS             1903..3000
                     /locus_tag="PKK76_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69105.1"
                     /translation="MLAQSEKEFTSLGQRATFNRTLRHLVSVTTIVDRVEKGENLADI
                     LNELLDSEMLARDQIGPVLSLVLVSKMDLPFRSRNLPESVNKFEGILAQMKKWPGVDL
                     VIAYHHPNLGIIPINPANEAHWNNAIELKRDELVVVYARAASKKTETAQKALDAFFTL
                     LKGGTPAEDPDFATKPVAAKPVAQRAAAAPAAAAQARPMPAPQARPMPGAPGAMPQPR
                     PMPAAPGAAQPQAPAPAPQEVAPKEAPKPAAAAAPAGARNLTPKYSVQVTNELFHNGN
                     VEAWKNIIESYEGSHPGCKVIVYHEGELIQDLNSLFKWGKVKHGGLLMFQVAGNAIKN
                     VSRLQKYLHEGASKRYETFMKHDMNRNLSLF"
     gene            3080..3736
                     /locus_tag="PKK76_11305"
     CDS             3080..3736
                     /locus_tag="PKK76_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69106.1"
                     /translation="MDKRGLVLPSKEKDISVYKDFYDFLKEHLPHQFSLATGKVRGKK
                     HILNRSIDCLVYWKLCNKIMEMTGGYILVDHLYSFITLEPELSTQQLITHATMTKGMK
                     ALYAATRDIPDNQILPVFSVLFAYRSTIPLLSHKVALEDTAREKEIPINHETDLVCIL
                     DQGVIVKDWESGGYRAVETGKDTLMWFYILLMEYLDRDGKVGFDAREYIKSSREYKEY
                     "
     gene            3740..4870
                     /locus_tag="PKK76_11310"
     CDS             3740..4870
                     /locus_tag="PKK76_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNL69107.1"
                     /translation="MLQRISLAIMSLALFAFANCGSNKDYLVVVDTSGSMQEKNTFKR
                     VKDSMPKLLDALKKGDTVTVMRFDTEVQGGSTIKISSDADRAAITKEIESMKAQGSYT
                     DMAKMLLAVREKVKAMKEEGKTAHIVVLSDGLDDPRPEGGRKRGTVDIGKYRDADGGP
                     IQDAFIYYISLGDISNPALEEQLKKLSPQTKTYLAGNRAGDAGKPGSVGKDGKPVQAT
                     PEQAMAEVGSDVSKNDWLSYVKKYWIYAAGLVLLGLLIYLAILLFRKLAEGESLYGTI
                     LYYPEGIQFPNKNTFTLDKVGKAAVTIGARQGNRLRIKDLGTNEVFSFKGKKVGPNLC
                     LKPLGKSARNIQFLSQRQQGLVSPGDRFRLGDYSFIFNDEEA"
BASE COUNT         1381 a         1241 c         1237 g         1059 t
ORIGIN      
        1 aaaccagtct tatgtgaaca tgttcaatcg ttacactgta acagttgaag atgagatggt
       61 caaaaaagtt cgcgagttca tcgacaaaga agaactccgt actctgcagc gttacactcc
      121 tcgcgagatc gttgagatcc tcgacaaagt aggtggcgcg gacaagttca acacagggga
      181 acttgagaag tcagtcgtga acatgtatgg gcaccttcag gggcacatcc agcgtggtat
      241 gaacgacctc gaaaacgaaa ccaactcgat tctgcgtcag aaaacagacg tgggcgcgtt
      301 tgttcgtggt gagaacgctt actccatcgt aaaatgttct ttcaaagaca gccagttcaa
      361 gccaaagact gtaacggaca tcaagctctc gatcaacatt ctggattcag aattgatcag
      421 cccgatcttc cactaccaga ctactgttga acacctgctg aaagactcaa tctccaagca
      481 catcactgaa ctgatcgatc gtgagatcga caagctcaaa gaagagctcg tggatacagg
      541 tcgtgcagaa ctgtcagacg gcgagatcat gttcgaaaag atcaagctcg tagacaagta
      601 tactgatgac aacgtatcgg atccgaattc tcgtcgttac acgattctgg ccaagaagtt
      661 cctcgataaa atcgaaggcc ttcgcgctga aatcgatcag gatgactatg actccatgaa
      721 catgcgcgag cagatcaaga agatcattga ctccgagaac attcgtaacc gcggtttcaa
      781 cactgcagtc aacactctga cttctattct tgatacttcc aaactgggtt accaggtctg
      841 cgataacaag aagaacgctc gtgagtgctg ggtgcgtgaa tacgaagatc aggacgatgc
      901 gcgtcttcca gacgaacgtt acgcgatccg tctcgtatac ctcgaccaga accagatccg
      961 cgacctcaag cgcgcatacg atgcacgcat cgctgcattc cttcgcgaag gcgaagcagt
     1021 gtgggatgtg gttcatgaag tgtatgaccg caccaagaag cgtttctttg gactgtcacg
     1081 tgtagtgaaa gacttcgaag acctggcccg ttctatcatg ccaaaagcat gggtaaaaga
     1141 acgcaccact gtgaacaacg atccggatga aatccactgg gacaagctcc gtttccgcga
     1201 tgcgccaact accttcgtag aaaagaacaa ccgtacttac tctatggaag tgaagaacgt
     1261 tggagaaaag atccgctacc tgcgtgaccg tctgcaaacc atgtatggtt atcagaaccc
     1321 ggttgagcgt gttgtgatcg acgaacgtct caaccttctc gaacaaacat tcaacaagta
     1381 catctatgaa atcaacccgc accacctcca gccgggcctg attctagacg tagacatcac
     1441 tacgacgaag cgcaagatgt acatgatgaa gtctatggca aacgttctga acgaattcct
     1501 gtcaggcgta tctcagggct tcgcggatgc agcgttcgct tccttcaagc gtcgtcgttc
     1561 gactgtccgt gcggacatcg aaatgacctt ctcgcaagaa gagcacaagg actccgcatc
     1621 ttccctggca tacaaaggcc aggagccagc ggcaggtagc atgtatgcag caccatccgc
     1681 tccagaacca caacgggcag catctgccgg tggtgaatcc ggagtccgtg gaagcgcaga
     1741 cgtaacacca aagtcctatg aggacaaact caaggagatg ggtctcaagg aactgtaaga
     1801 ttccaaaaag actctgaaac ctgaaaaccc gcaggatagc ctgcgggttt tttatatgca
     1861 gaaatggaca gtctttgtta gtagggtcat ccggaggcca caatgctcgc tcaatcagaa
     1921 aaagaattca catcgcttgg acaacgcgct acgttcaatc gcactcttcg ccatctagtc
     1981 agtgtaacca caatcgtgga ccgcgtggag aagggagaaa acctcgcaga cattttgaat
     2041 gagcttctcg attcagagat gctggcgcgc gatcaaatcg gtcccgttct ttctctcgtt
     2101 ctggtcagca aaatggacct tccatttcgc tctcgcaatc ttccagaaag cgtaaataaa
     2161 ttcgaaggca ttctcgcgca gatgaagaag tggccaggcg tggatctggt gattgcatat
     2221 catcatccga acctgggcat cattccaatc aatcctgcca atgaagcgca ctggaacaat
     2281 gcgatcgaac tcaagcgtga cgagctcgtt gttgtatatg cgcgcgctgc cagtaagaaa
     2341 acagaaaccg cacaaaaggc tctggatgcg tttttcactc ttctaaaggg cggcaccccc
     2401 gcagaagatc ctgatttcgc aaccaaacct gtagcggcca agcctgttgc acaaagagcg
     2461 gcggcggctc ctgcggcagc agcccaggcg cgcccaatgc ccgcgccaca ggcgcgccca
     2521 atgccaggcg ctccaggtgc tatgcctcag cctcgtccaa tgcccgcagc accgggggcc
     2581 gcgcagccac aagcacctgc tccggcaccg caagaagttg ccccgaaaga agccccaaag
     2641 ccagcggctg cagctgctcc ggccggagca cgaaatctga ctccgaaata ttcggtgcag
     2701 gttacaaacg agctctttca caatggaaac gtggaggcgt ggaagaacat catcgaatca
     2761 tacgaaggat cgcatcctgg ctgtaaggtg attgtatacc atgaaggcga actgatccag
     2821 gatttgaact ctctgttcaa atgggggaaa gtgaagcatg gtggtctcct gatgttccag
     2881 gtggcaggaa atgcgatcaa gaacgtaagt cgtctgcaga agtatctgca cgaaggtgct
     2941 tccaagagat acgagacgtt catgaaacat gacatgaatc gtaatttgag tctcttctag
     3001 ttcgcctgcg aaaagaacga tgttcggaga gcattttcag aagcagacgg atctaatcac
     3061 cagaggtctg aaggagagaa tggacaagcg tgggcttgtc cttccttcca aagaaaaaga
     3121 tatctccgtc tacaaagact tctacgattt cctgaaagag caccttcctc accagttttc
     3181 cctggcaacg ggcaaggtgc gcggaaaaaa gcacatcctg aaccggagca tcgactgcct
     3241 ggtgtactgg aagctgtgta acaagatcat ggagatgacc gggggttaca tcctcgtaga
     3301 tcatctttat tctttcatta ctcttgagcc ggaactgtct acacaacagc tcatcacgca
     3361 tgcaaccatg accaagggca tgaaggctct gtatgccgcc acaagagaca tcccggacaa
     3421 tcagatcttg ccggtgtttt ctgtgctttt tgcctatcgt tctaccattc cgcttctttc
     3481 gcacaaggta gccctggaag atacagcgcg ggagaaggaa attccaatca accatgagac
     3541 ggacctcgtt tgcattctgg atcagggcgt aatcgtcaag gactgggaga gcggcggtta
     3601 ccgcgctgtg gaaacgggca aagataccct gatgtggttc tacatccttc ttatggagta
     3661 tcttgacagg gatggaaagg taggattcga tgctagggaa tacataaaat cttccagaga
     3721 atataaggaa tactgaatca tgctccagcg aatctctctc gcaattatgt ccctcgctct
     3781 ttttgccttc gcaaactgcg gaagcaacaa agactatctg gttgtagtgg atacttccgg
     3841 gtccatgcag gaaaagaaca ccttcaagcg tgtaaaagac agcatgccca aactactcga
     3901 tgcgctgaag aagggtgaca ctgttaccgt tatgcgcttt gacacagaag tgcagggcgg
     3961 ctccacaatc aaaattagca gcgatgcaga tcgcgccgct atcacgaaag aaatcgaaag
     4021 catgaaagcg cagggctcct acacggacat ggcaaagatg cttctcgctg tgcgcgaaaa
     4081 agtcaaggcc atgaaagaag aaggcaagac ggcgcacatt gtggttctgt cagatggtct
     4141 cgatgatcca cgcccggaag gtgggagaaa acgcggcacc gtggatattg gaaagtatcg
     4201 cgatgcagac ggtggtccca ttcaggatgc attcatttat tacatcagtc ttggtgacat
     4261 cagcaatcct gctctagaag agcagttgaa gaaactttct ccacagacaa agacgtatct
     4321 ggcaggcaat cgcgcgggag atgcaggcaa accaggttcc gtaggcaaag acggaaaacc
     4381 agtgcaggcc actccggagc aggcaatggc tgaagtagga tcagacgtaa gtaagaacga
     4441 ttggctcagc tatgtgaaga aatactggat ctacgctgca gggctcgtcc ttcttggatt
     4501 gctcatctac ctggccatcc tcctgtttcg caaactcgca gaaggcgaat cactctatgg
     4561 aaccatcctc tactatccgg aaggaattca attcccgaac aagaatacat tcactctgga
     4621 caaagtggga aaagcagcgg ttacgattgg ggcgcgccag ggaaatcgtc tgcgtatcaa
     4681 agacctgggc acaaacgaag tgttctcatt caaaggcaag aaagttggcc cgaacctctg
     4741 cctgaagccg ctcggcaaga gcgcacgcaa cattcaattc ctgtcgcagc gccagcaggg
     4801 attggtgagt ccgggtgacc gattcagact tggcgactac tcttttatat ttaacgatga
     4861 agaagcctga aaattactta gcagagttac gccagagcga tcgtatcttt cgtctgga
//
LOCUS       DAORFF010000268         4918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9692_length_4918_cov_3.195558, whole genome shotgun sequence.
ACCESSION   DAORFF010000268 DAORFF010000000
VERSION     DAORFF010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9692_length_4918_cov_3.195558"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKK76_11315"
     CDS             complement(<1..322)
                     /locus_tag="PKK76_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69108.1"
                     /translation="MIKLTSDFRKVLSFHLGAGVIWTLIAIVFGLLNQLQLMLGWPQP
                     DVLGYGLIRPLFTTSLIFGGIVNLSLAGSYYVMQKSGDLAWQPLALAGFGLQQLGLLL
                     GVIGI"
     gene            complement(319..1227)
                     /locus_tag="PKK76_11320"
     CDS             complement(319..1227)
                     /locus_tag="PKK76_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNL69109.1"
                     /translation="MPALASIEGITLKSLIITPAAIALFLIGCGQKTPPVYESSQDQI
                     FLGSVMYSQENCKNCHGNAWDGKGPDAESLGVPVPNFTSAVGPEKTPTSYFKAITVGT
                     DKVKNHAYQSLTDKGRWALAHFLYSLKKPLAGEKADVQRKNIEAGMTEARDAYAKTRR
                     WNMGFQPLDQREKAPDLAELVKIADLKPEGGPGAVSDERKARPHNGEGASLYKNNCES
                     CHGIYGEGRNLTFGMGLIASKNDFYGFKKTRATISTRDLASSGSTSAGGLKSAHGSGS
                     LLLNNFDSFTDAQWSALSDFIRGMAQ"
     gene            complement(1206..2489)
                     /locus_tag="PKK76_11325"
     CDS             complement(1206..2489)
                     /locus_tag="PKK76_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69110.1"
                     /translation="MKLPEEKALNFQLSPKLKIVCMALIAFGLATLAYQIAFPWHPPV
                     AEVAEGHAAAGEHAAGNPRLFMSLLLALLVALPLGLGGLYFVAFNHLAGAAWCVTIRR
                     IAESYFPYLLVVLALMAAVFFGMKNVYSHWMLTTEGGHADIADHLLQVKRPWLNPGFF
                     VTRNFAFVLLWLGFGFVLWKKSTDQDTDGQVEHTRSLAKWSAVFVILFALTYSASAWD
                     LTMSLEPHWFSTMWGPYCFAGLALTAFSSLILWIWYLKSSGYFGDALNENHYHDMGKY
                     MWGHSIFWAYVMICQYLLIWYAHIPEETIFYRKRIYTPEIGFQPWAIVSLLLILVRFV
                     IPFFFIIQRKAKRNIKHLGIGACIVLLGQVIDMYWVTYPTLANGDFVMFDLRELGTIS
                     LVAGSFILVTAWSLSRKSLVPKGDPRLEECLHWHQ"
     gene            complement(2491..3138)
                     /locus_tag="PKK76_11330"
     CDS             complement(2491..3138)
                     /locus_tag="PKK76_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNL69111.1"
                     /translation="MTQEEKKALITRWIVAAAFMAVFWTVIIIVTGGRYHSNVSGLHY
                     FLDMHDTSMVRAQREDTTTLENVKQALFKGADQNEVWGGPGSSMRVPPEGTVPRGYEP
                     YPYEQAEIDLAGANLKNPLPLTQTVLERGKDRFETYCAVCHGMGGQGDGTVIPRMIQP
                     PNLITGQSAGWQDGKIFHMITMGRGNMLPYAAPIRPADRWAIVHYVRLLQKKGAK"
     gene            complement(3147..3818)
                     /locus_tag="PKK76_11335"
     CDS             complement(3147..3818)
                     /locus_tag="PKK76_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNL69112.1"
                     /translation="MLATIDRLFQPLEGILDRVFGAILPKLHNTLDRLFEYQYNETRK
                     GVFGLFDTPEKIKKAAKAAHEKGYTGFDCFTPFPVHGLEFDMGLKRSKIPYVTFFMGM
                     CGTLTAFFLQFGVHEQISTIPYMNSYPLNIGGKPTFSWPAMVPICFELTVLFGGQCTA
                     ATLLLLSKLPRPFRKPLHKEITNDKFALWIPAHSAGYAEEDVKSFLSSIGAASVTAVD
                     EKKPF"
     gene            complement(3821..>4918)
                     /gene="nrfD"
                     /locus_tag="PKK76_11340"
     CDS             complement(3821..>4918)
                     /gene="nrfD"
                     /locus_tag="PKK76_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNL69113.1"
                     /translation="AILFVFRQQWRTGINRSAEAMTIFSVMTAASFILLHVGRTWVIF
                     WGLPIPNERGVLWPNFRSPLAWDEFAISTYFITSLVFWYIGLVPDFASIRDRIDASTT
                     GQKVRKVVYSILSFGWVGSARAWSHYEATSMILAGLSMPLVFSVHSIVSFDFATSVIP
                     GWHATIFPPYFVLGAVFSGFGMVLTLLIIMREVFRLTEYITIKHLENMAKIVTLTGSL
                     VGLAYGTEFFIAWYGGSQFEGFTFINRAFGPYWWSYWIMISCNVISPQLFWFRAIRTN
                     PVALWIISIFVNIGMWFERWVIIVTSLHRDYLPSSWDMFIPTIHDYLILIGSFGMFFT
                     FFLLFTRFFPAIAIAEIKATIKSPDAKGFGH"
BASE COUNT         1263 a         1191 c         1300 g         1164 t
ORIGIN      
        1 gaatgccgat tactccaaga agcagaccca gttgctgcag tccaaagcct gcgagcgcca
       61 gtggttgcca ggcaagatcg cctgacttct gcattacata gtaagaaccg gccagagaga
      121 gattcacaat tccgccaaag atcagagaag ttgtgaaaag tgggcggatc agtccgtatc
      181 ccagaacgtc tggctgcggc cagccaagca tgagctggag ctggttgagc agaccaaata
      241 caattgcaat caaagtccag atcacacctg cgccgaggtg aaacgaaagg acttttctaa
      301 aatcagatgt aagcttaatc attgcgccat acctcgaatg aagtcgctga gtgcgctcca
      361 ctgagcatct gtgaagctgt cgaaattgtt cagaagaaga ctgccgctac cgtgagccga
      421 cttgaggccg ccagcgcttg ttgagccgga gcttgccaga tcgcgagtgc tgatcgtcgc
      481 gcgagtcttc ttgaagccgt agaaatcatt cttggaagca atcagtccca ttccaaatgt
      541 aagattgcgt ccttcgccat agatgccgtg gcagctttcg cagttgttct tgtagaggct
      601 tgcaccttca ccgttatgcg gtcttgcttt gcgctcatcg ctcacagccc ctggtccacc
      661 ctcaggcttg agatctgcga ttttgacaag ctctgcgagg tccggagctt tttcgcgctg
      721 atccagaggc tggaagccca tgttccatct gcgagtcttc gcgtacgcgt cacgggcttc
      781 cgtcattcct gcttctatgt tcttgcgctg cacgtctgct ttttcgccag cgagcggctt
      841 cttcagagaa tagaggaaat gtgcgagtgc ccagcgacct ttgtctgtca gagactggta
      901 ggcgtggttt ttgactttgt ctgtgccgac tgtgattgct ttgaaatagg aagttggagt
      961 cttttcaggt ccgaccgcag aagtgaaatt tggaaccggc actccaagtg attctgcatc
     1021 cgggccctta ccatcccagg catttccatg acaattctta cagttttcct gcgaatacat
     1081 cacggaaccc aggaagatct ggtcttgcga agattcgtag accggcggtg ttttctggcc
     1141 gcagccaatc agaaaaagtg cgatggctgc gggagtaatg atcaacgatt tcaatgtgat
     1201 accctctatt gatgccagtg caggcattct tccagtcttg gatcgccttt gggcacaaga
     1261 gacttacggc tgagtgacca ggccgtaact agaatgaagc ttcccgcaac gagtgatatg
     1321 gttccgagtt cgcgaagatc gaacatgacg aagtctccgt tcgcaagggt tgggtaagtg
     1381 acccagtaca tatcaatcac ctggcccaga agcacaatgc atgcgccaat gcccagatgt
     1441 ttgatgttgc gttttgcttt gcgctggatg atgaagaaga aaggaatcac gaagcgcacc
     1501 agaatcaaca gcaggctgac aatggcccat ggttggaatc cgatctctgg agtgtagatt
     1561 ctctttctgt agaagatggt ttcttctgga atgtgtgcat accagatgag taggtactga
     1621 cagatcatca cgtatgccca gaagatcgaa tgtccccaca tgtatttgcc catgtcatgg
     1681 tagtggtttt cattcaatgc atctccgaaa tagccggagc tcttgagata ccagatccac
     1741 agaatgagag atgagaaagc agtgagtgca agtcctgcga agcagtaagg gccccacatt
     1801 gtggaaaacc agtgtggttc gagactcatg gtcagatccc acgcgcttgc agagtaagtg
     1861 agtgcaaaaa gaattacaaa taccgctgac catttcgcaa gagatctggt gtgttcaacc
     1921 tgtccgtctg tgtcctggtc agttgatttc ttccagagca caaatccaaa tccaagccac
     1981 aacaacacga atgcgaagtt acgcgttaca aagaatccag ggttgagcca gggtcttttg
     2041 acctggagta agtggtccgc aatgtctgca tgaccgccct ctgttgttag catccagtgg
     2101 ctgtatacat tcttcattcc aaagaagact gcagccatca gagcgagaac cacgagaagg
     2161 tatggaaagt agctttccgc gatgcggcga atggtaacgc accacgcagc acccgctaga
     2221 tgattgaaag cgacaaaata gagtccgccc agaccgagcg gcaatgcgac cagaagcgca
     2281 agcagcagag acatgaacaa tctgggattt ccagctgcgt gttcccctgc ggcggcgtgt
     2341 ccttccgcca cttctgctac gggcggatgc catgggaaag caatctggta tgcgagagtc
     2401 gcaagaccaa acgcgatcaa cgccatacag acgatcttga gttttggtga gagctgaaaa
     2461 ttcagcgctt tttcttcagg aagtttcata ttactttgcc cccttcttct ggaggagcct
     2521 tacgtagtgt acaattgccc agcggtctgc cggacgaatg ggagcagcat atggaagcat
     2581 gttaccgcgt cccattgtga tcatgtggaa gatttttccg tcctgccagc ctgctgactg
     2641 acctgtgatc aggttgggag gctggatcat gcgagggatt acagttccat cgccctgacc
     2701 gcccattcca tggcagacag cgcaataggt ttcaaaacga tcttttcctc tttcgagtac
     2761 agtctgcgtg agaggaagag gattcttcag atttgcgcct gccagatcga tctcggcctg
     2821 ttcgtatgga tatggttcat atccgcgggg cacggttcct tccgggggca cacgcatcga
     2881 acttccaggt ccgccccaga cttcattctg gtctgcgccc ttgaagagtg cctgctttac
     2941 attttccaga gtcgttgtgt cttctctctg cgctcgcacc atcgaggtgt cgtgcatgtc
     3001 caggaaataa tgaagaccag aaacgttgct atgataacgt ccgccggtga caatgatgat
     3061 cacggtccag aacaccgcca tgaacgctgc ggcaacgatc cagcgggtaa tgagtgcctt
     3121 tttttcttcc tgcgtcatac gattcctcag aatggtttct tctcatccac cgcagttaca
     3181 gatgctgctc cgatggaaga aaggaatgac ttaacgtctt cctccgcata accggcagaa
     3241 tgcgcgggaa tccagagtgc gaacttgtca ttagtgattt ctttgtgcag aggcttacga
     3301 aatggccggg gcagtttgct cagaaggagc agggtggccg cggtgcattg tccgccaaac
     3361 agaactgtca gttcgaagca aatcggaacc atggccggcc agctgaacgt tggtttgccg
     3421 ccgatgttga gaggatagga gttcatgtaa ggaatcgttg aaatctgctc atgaacgccg
     3481 aactgcagga aaaaagcagt caatgttccg cacattccca tgaagaatgt aacatacgga
     3541 atcttggaac gcttcagtcc catatcgaat tcaagtccgt gtactggaaa cggagtgaag
     3601 cagtcaaagc cagtgtaacc tttttcgtgc gcagctttgg ccgctttctt gatcttctcc
     3661 ggtgtatcaa ataatccaaa aacacctttg cgtgtttcgt tgtactgata ctcgaacagt
     3721 cgatccagag tattgtgcag ttttggaaga atggccccga acacacggtc caggattcct
     3781 tccagtggtt gaaagagacg atcgatggtt gccagcataa tcagtgtcca aatcctttcg
     3841 cgtcaggcga tttgatagtc gctttgattt cagcgattgc aattgccggg aagaagcgag
     3901 tgaacagtaa gaagaatgtg aagaacattc caaatgatcc aatcaggatc aagtagtcgt
     3961 ggatggtcgg gatgaacatg tcccagctag aaggaaggta atctctgtgc agagaagtca
     4021 cgatgatcac ccagcgttcg aaccacatac caatgtttac gaagatagaa atgatccaca
     4081 gagccaccgg atttgtgcga atcgcacgga accagaacag ctgtggtgaa atcacgttgc
     4141 aggaaatcat gatccagtag gaccaccagt acggaccaaa tgcgcggttg atgaaagtga
     4201 atccttcaaa ttgagatcca ccataccatg cgatgaagaa ttcagttccg taggcaagac
     4261 ccacgagaga gcccgtgagc gttacgatct tcgccatgtt ttcgaggtgt ttgattgtga
     4321 tgtattcagt gaggcggaac acttcgcgca tgatgatgag cagagtgaga accatgccga
     4381 atccagagaa tactgcaccc agaacgaagt aaggagggaa gatcgtcgca tgccatccag
     4441 ggatgactga ggttgcaaag tcgaaggaaa cgatggagtg tacggagaat acgagaggca
     4501 tggacaatcc tgcaaggatc atcgaagttg cttcgtagtg gctccacgct cttgcagatc
     4561 caacccatcc aaatgaaagg atagagtaga ccaccttgcg cactttctga cctgttgtgc
     4621 ttgcgtcgat tctgtcgcga atggacgcga agtcaggaac cagaccaatg taccagaaca
     4681 cgagagatgt aatgaagtaa gtagaaatcg caaattcgtc ccaggcgaga ggggagcgga
     4741 agtttggcca gagaacgccg cgttcgtttg gaattggaag gccccagaag atgacccagg
     4801 tacgtcctac gtgcagcaga atgaaagaag cggccgtcat cacagagaag atcgtcattg
     4861 cttctgcgga gcggttgatc cctgttctcc actgctggcg gaatacaaac agaatcgc
//
LOCUS       DAORFF010000269         4912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9710_length_4912_cov_4.166564, whole genome shotgun sequence.
ACCESSION   DAORFF010000269 DAORFF010000000
VERSION     DAORFF010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9710_length_4912_cov_4.166564"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..651)
                     /locus_tag="PKK76_11345"
     CDS             complement(1..651)
                     /locus_tag="PKK76_11345"
                     /inference="COORDINATES: protein motif:HMM:NF028114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5071 domain-containing protein"
                     /protein_id="HNL69114.1"
                     /translation="MKRYATSPDAPMTTYILYESKVNCSFTFLPEDQSGPTEPDDVEV
                     WRVEAPSFEIACLKRNEYLGWEPYRPQICSEHDLHALLPRSKHDLDNVQLLMNAGLPE
                     IKPVLPQLFEWIQDINWPVARLLLPFLRSLGSKCREEIQSVLQSDDAMWKYWIITELI
                     AKMDQIEARAFMPDLENLLATATLEDKNNEVEAVAAEVLRALRSGKDSSSIDAAGR"
     gene            complement(667..1203)
                     /locus_tag="PKK76_11350"
     CDS             complement(667..1203)
                     /locus_tag="PKK76_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69115.1"
                     /translation="MLKMIGVLSLVFGVGLGCTQQLPRGEIRSLRTIGKPIPNSGEFT
                     FDAENMVDGNLTTSWQTSTPHDSGVLVEFNNPVNLVKLRIANGFQFMNHPRFGNLLEK
                     NSRVRGMALAMEVKDSDGSISTVTKEIELRDRTEAGYDDVALEQNNVLRIAFKPLSIY
                     PGSKWADLAISEVIFFKK"
     gene            complement(1277..1921)
                     /locus_tag="PKK76_11355"
     CDS             complement(1277..1921)
                     /locus_tag="PKK76_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNL69116.1"
                     /translation="MHNEKLATTRTTPCSTFKIWNTLIGAENHYLLSPDQPFYHWDGT
                     VREIPEWNKDLTLSEAFKVSCVPAFQDLARRTGRAKMETWIRLMPYGNGDLSSGIDDF
                     WLPRPGKASIQISPFEQALLMRRLVRGELPFSESSRNLLYKIMLVKKTPGGKLYGKTG
                     TGSLESGENLGWFVGFVQSSTKRFTFACLVKSHDASGAVARALTIQILEDSGML"
     gene            complement(2080..2373)
                     /locus_tag="PKK76_11360"
     CDS             complement(2080..2373)
                     /locus_tag="PKK76_11360"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNL69117.1"
                     /translation="MILEIAMLQVRPGLTQEFESNFAVASKILSSMPGYLGHSLRRCI
                     EDKNKYALLITWETLEAHTEGFRKSSGYLEWKALLHHFYDPFPIVEHFEDLSP"
     gene            complement(2611..3171)
                     /locus_tag="PKK76_11365"
     CDS             complement(2611..3171)
                     /locus_tag="PKK76_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69118.1"
                     /translation="MRFIKLLSLLLLTQCLTFNPIPGKEAAIDSAQIKLLSIFGPMDT
                     FSYGQFTYSPRDVTQAMWVFNVQYSGTGKVDPVAVSLEEYTMVDKGKESLNMPVRVHE
                     CCYSNSFGFSTNPFQNLANIVNRSRAGGSFMPDAAIPLQGGEPERRMLVFVFPRNKTP
                     TKLSFHPLRDVKDETAKIDLGLTNTP"
     gene            complement(3325..3588)
                     /locus_tag="PKK76_11370"
     CDS             complement(3325..3588)
                     /locus_tag="PKK76_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69119.1"
                     /translation="MLKKTANYLLLYGLLTFVFTAIYSRFTSSAYALGTATGIAFVSL
                     VLPFFIFGIRAIFNRKLSPRVITVSRFLWLVCWAGAVLRQVLR"
     gene            complement(3674..4144)
                     /locus_tag="PKK76_11375"
     CDS             complement(3674..4144)
                     /locus_tag="PKK76_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69120.1"
                     /translation="MNWREHPILKKLSPPDYPDDVPAIIHEGFRAATRTPEKVWIRIS
                     DMRDSILTGTLLNAPHQLPSLSQGKVYSFQLSGAGIPILVSESYLREIVDWRFAIACK
                     KCGNDVLFDNPSTIIRMAFPNMPEGSLMEAFSTFCPLCHDPIVLRHRNAPPDEP"
     gene            complement(4288..4569)
                     /locus_tag="PKK76_11380"
     CDS             complement(4288..4569)
                     /locus_tag="PKK76_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69121.1"
                     /translation="MNSTIRPFIVLIIAFAVTFCNGRQQFDPTGSREAEWKRRCQLNA
                     FIALSAMCAAYASRQTATAAYDPFTACFYESSFAFGSSCTEAAFRKNNE"
BASE COUNT         1228 a         1172 c         1259 g         1253 t
ORIGIN      
        1 ctatcgacca gcggcatcaa tgctagacga atcttttcct gaacgaagag cccgcaaaac
       61 ttccgcagca accgcctcta cttcgttgtt cttatcttcc agggttgcag ttgccaggag
      121 attctcgagg tcaggcatga aggctcttgc ttcgatctga tccatttttg cgatcagctc
      181 ggtgatgatc cagtacttcc acatggcatc atccgactgt aaaacgctct ggatctcttc
      241 cctgcatttt gagccgagag accgcaggaa cggcaatagc aaacgcgcga ctggccagtt
      301 gatgtcctgg atccactcga acagctgtgg aagcactggc tttatttccg gcaatcctgc
      361 attcataagt aactgcacat tgtccagatc gtgtttggac cgaggcagca aagcgtgaag
      421 gtcgtgttcg gaacagatct gtggtctgta tggttcccag cctagatatt catttctctt
      481 gagacaggca atctcaaagg agggagcttc tactctccat acctcaacat catccggctc
      541 cgtaggtcca gactggtcct ctggtaggaa cgtgaatgag caatttactt tcgattcgta
      601 tagaatatat gtggtcattg gggcatcggg agatgtcgca taacgtttca tctcccgaca
      661 agtcatctat ttcttaaaga agatcacttc gctgatcgct agatccgccc atttggaccc
      721 tggatatatg gagagaggct taaaagcaat cctcagtaca ttgttttgct ccagagcgac
      781 gtcatcgtat ccagcttccg tgcgatctct cagttcgatt tctttggtca ctgtggagat
      841 ggacccatcg gagtctttta cctccatggc gagcgccatg ccgcgaacac gcgaattctt
      901 ttccaggagg ttgccgaatc tggggtgatt catgaactgg aaaccatttg cgattctcag
      961 tttcaccagg ttcaccgggt tattgaattc cacgagcact ccggagtcgt gaggcgtaga
     1021 agtttgccag ctggtggtca gatttccatc caccatgttt tccgcgtcaa aggtgaattc
     1081 tccagaattg gggattggtt ttccgatggt tctaagactt ctgatttcac cgcgaggcag
     1141 ttgctgggta cacccgagcc ccactccaaa tacaagactc aatacaccga tcattttcaa
     1201 catttgagat tctcctttat tcgcccggaa ctctttgaac aacatgggaa cgaaaaatgg
     1261 cgagcaaatt ttgtgttcag agcatccccg agtcttccag gatctggatt gtgagtgctc
     1321 tggcgaccgc accgcttgca tcatgagact tgaccaaaca ggcaaaagtg aatcgtttgg
     1381 tggaagattg cacgaatccc acaaaccagc ccaggttttc gccactttcc aaagagccgg
     1441 ttcctgtttt gccgtagagc ttccctccgg gggttttttt taccagcatg atcttatata
     1501 gtaaatttct tgaactttcc gagaaaggca actcgcctct caccagtctt cgcatcagga
     1561 gagcctgttc gaatggagag atctggatgg atgccttgcc agggcggggc aaccagaagt
     1621 cgtctatgcc agaagacaaa tccccgttgc cgtatggcat gagacgaatc cacgtttcca
     1681 ttttcgcgcg tcccgttcgc cgggctaaat cctgaaatgc gggaacacag gacaccttga
     1741 atgcctcgga cagtgtgagg tccttgttcc attctggaat ctcacgcact gtcccgtccc
     1801 agtgatagaa tggttgatca ggagacaaaa gatagtgatt ctcggcacct atcagagtat
     1861 tccagatctt gaatgtagag cagggggtgg ttcgcgtcgt ggccagtttc tcattatgca
     1921 tgaatctttg ctctgagtcc agatctagca ggacaaaagt tgcgttccgg ttttgaagat
     1981 gattctccat agaacgtgct atggattttt tttcagagca atcgcagatg acgcacaacg
     2041 tcaaaaaata tagcatccac gtttttttca agggttcgct caaggactaa gatcttcgaa
     2101 gtgctctact atgggaaagg gatcgtagaa atgatggagc aacgccttcc actcaaggta
     2161 tccggaggat ttgcgaaagc cttctgtgtg tgcttccaga gtttcccacg taatcaacaa
     2221 agcgtattta tttttgtctt cgatacatct tcgaagcgaa tgacccaggt atccgggcat
     2281 cgaggacagg atctttgagg cgactgcgaa attggattcg aattcttgtg tgaggccagg
     2341 tcgtacctga agcattgcaa tttcaagaat catatggttc gaccactgct agcggtaagg
     2401 caggcgcaaa tgtagagttc atgacgcctt gcaatctcct gatacccggc gaaccgtgcg
     2461 cgaaggacca gggagaatca tgcatatcgc attctttcag gctcaatagg agtctgtggc
     2521 gattccagta ttcatgcgcg agtcaaagtc caattccgca aactgccacg attagtcctg
     2581 gcccggctaa gccgggcagg tttcgcgctg ttacggagtg ttcgtcaaac cgaggtcgat
     2641 tttggccgtt tcatctttca cgtcgcgcag tgggtgaaag cttaatttcg ttggtgtctt
     2701 gttgcgcgga aaaacgaaga caagcatgcg gcgctctggc tcgccgccct gtagagggat
     2761 agcagcatct ggcatgaagc tcccgcccgc cctggaacgg ttgacgatgt ttgcaaggtt
     2821 ctgaaaggga ttggttgaaa agccaaaaga atttgaatag cagcattcgt gtacgcgaac
     2881 cggcatattc agggattctt tgcccttatc caccatcgtg tattcttcca atgataccgc
     2941 cactggatcc acttttcccg ttcctgaata ttgaacattg aaaacccaca ttgcctgagt
     3001 gacatcgcgc ggactgtaag tgaattggcc gtagctaaat gtgtccatag gtccgaaaat
     3061 cgatagcaac ttgatctggg cagagtcaat ggctgcttct tttcctggta tcgggttgaa
     3121 ggtgaggcat tgagtcaaaa gcagaaggct gagaagtttg atgaaacgca tcagcaatac
     3181 agaatttgaa gcgaaaccgc gtcaaccaca atggatccaa accagaaggt gtgccgagtg
     3241 cttttgttta gtggaacaga acgtttgaat gggacatgat tcagtgcgct ccaagaacgc
     3301 tcagcagtgc tgagaatggg ccggtcatcg gagtacctgg cgaagtacgg cacctgccca
     3361 acagacaagc cagagaaatc ggctgaccgt gataacgcgc ggcgatagct tgcgattaaa
     3421 tatggcccga attccaaata tgaaaaacgg aagaaccaga gagacaaaag caatgccggt
     3481 ggcagtgccc agggcgtagg ccgaactggt gaaccgagaa taaatggcag taaagacaaa
     3541 cgtgagcagt ccgtacaaca acaaatagtt tgcagttttt ttcaacatgg tttttccgtg
     3601 cgacgctagt gtgagaccac tcggtccgcg gttttcagat tcgactgcgt gtcgagggga
     3661 atgttccggt cttctaaggt tcgtcaggtg gcgcattcct gtgacgaaga acgattggat
     3721 catgacacag ggggcagaaa gttgaaaagg cctccatgag actcccttca ggcatgtttg
     3781 gaaatgccat tcgaataatt gttgatgggt tatcaaacag aacgtcgttg ccgcacttct
     3841 tgcatgcgat ggcgaaacgc caatcaacaa tctcacgcaa gtagctttcg gaaacgagaa
     3901 ttggaatgcc cgcacccgag agctgaaacg aataaacctt gccctggcta agtgacggca
     3961 attggtgcgg cgcattcagc agcgttccgg tgaggatgga gtctctcata tctgaaatgc
     4021 ggatccagac cttttctgga gtacgagtgg cggcgcgaaa accctcatgg atgatggccg
     4081 gtacgtcatc aggatagtcg ggcggactta atttcttgag gatgggatgc tctctccaat
     4141 tcataaaaga ctccggtgga atttctcagt gtgcttgcat agatttcgaa attgagtcaa
     4201 acactttgcg acacaatgcc aagtattggc tgcgccggac ctatcagaaa ctaccaacgc
     4261 ctgacatgag ttacgcgaag tgccatgcta ttcattgttc tttctgaatg cagcttccgt
     4321 gcagctggaa ccaaaggcga atgacgactc gtaaaaacag gcggtaaacg gatcataagc
     4381 tgcggttgcg gtttggcgac tcgcataagc tgcacacatg gcggaaagag cgataaaggc
     4441 gttcagctga caccgccgtt tccattcagc ttctctggat cccgttgggt caaattgctg
     4501 gcggccattg cagaaggtca ctgcaaaagc aatgataagc acgatgaacg gtcgtatggt
     4561 ggagttcatt caggttgacc cgcaaggttt tggatgcatg ccggagtttc atcgaggatg
     4621 gtcgtggcag aattggttct atttatctga atggttcgaa acaatcgtag ctcctttgtc
     4681 ttcatccatg atcgagcatt ggttcacgtt gagaaggaaa taacatacct cgcttcctcc
     4741 gtttggatcg ccggatctgc atttcgcata acaggagtta tgcgccatat tcgcggcggg
     4801 ggaatcggaa ctgctttatc gcgccccttc acaaatccaa aatcttggtc attcacgggc
     4861 ttctgggaac gcttgacgac tgcggtaaaa aggccttcct agtcccactt tg
//
LOCUS       DAORFF010000270         4910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9717_length_4910_cov_3.795675, whole genome shotgun sequence.
ACCESSION   DAORFF010000270 DAORFF010000000
VERSION     DAORFF010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9717_length_4910_cov_3.795675"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..638
                     /locus_tag="PKK76_11385"
     CDS             120..638
                     /locus_tag="PKK76_11385"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNL69122.1"
                     /translation="MEVTDGRQFAVYMRTVRKRTLDVVQKVPIDRSQWRLSPDSMSVV
                     DIILHIASVEQALWGLDMATGTPGKLREFEKDSMNLSDAIATMANVRKGSSEFWSSLT
                     TTQLEQDILTPTGHSLPLKRWLVLAAEHEIHHRSFIHAYRKIWGLASSPIYGLTLDEL
                     RQHLEPKPASDS"
     gene            complement(714..1103)
                     /locus_tag="PKK76_11390"
     CDS             complement(714..1103)
                     /locus_tag="PKK76_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69123.1"
                     /translation="MNRNLYSILALVFAFSLYGQPGSGMGKNYDASKEVTVSGTVTEI
                     TRVPHGDMREGIHLAMSAGNETLVVMLGPTFYLEKQSVKVLKGDSITVIGSRTGNAII
                     ARQVTKGSQTLKLRTETGAPVWGRGGR"
     gene            1207..1470
                     /locus_tag="PKK76_11395"
     CDS             1207..1470
                     /locus_tag="PKK76_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69124.1"
                     /translation="MNYIKPLIAILVLSFARCGYEDIPNPRTGKTERQYQREKDQRNA
                     RKRIEFCTITVLVCQSMTPPGGTAPGGCNFTLSYVCTDYTNTI"
     gene            1570..2022
                     /locus_tag="PKK76_11400"
     CDS             1570..2022
                     /locus_tag="PKK76_11400"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNL69125.1"
                     /translation="MSNTGTVRLHRVLRAPPDRVYKAFVDKQALERWLPPYGFTGAIG
                     EMDVRVGSGYKMSFTNFSGGGSHSFSVKFVELVPAERIVHTDRFDDPNLPGEITVTIQ
                     LKKVSCGTDLSIVQEGIPAVIPEEMCYLGWQESLDQLTRLVEPEISAN"
     gene            2050..4233
                     /locus_tag="PKK76_11405"
     CDS             2050..4233
                     /locus_tag="PKK76_11405"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL69126.1"
                     /translation="MNHQKVERPGKIAFCVGWSIFSMLASVSSPVRAQSGLSIQTIPD
                     LSVMTSLDTTTQNSWWLATEPIDADRETKDAVAICNQKMIDRPALHWKQITVPGIIER
                     QTNFRGSTFWYRRLFRLSGAPEASVAVRLGRINDRDQTYLNGVLIGATGEWDSTIPAG
                     YDRTRIYGIPDNLLNRRGCNVLLIHVKRYFAESSGIATGVTVIGPASSITHEYFLEMI
                     RTSWMLGAYPALGLLFLIIYAVRRKQTEYLYFGLFTILLTLYELFRFDVKFELGISFF
                     ATKRIEYVLLYCLFPIFTAFLDTYFDAASLFRTRSIRRVILAANLYLICFILPTLLSN
                     DVDFWWTLQSHFIQPVWPVLFGYCLLILALQSMRGRKDALLLVPATLLLMAAVVLDIL
                     HAWNVVIHLPPLTIYAFSIFILSIAFLLAQRFAQSFSAVETTMSNLNATNAAYSRFVP
                     AEFLQILGKREITEVALGDQTLREMTILFSDIRSFTVMSEAMTPEENFNFLNSYLKRM
                     QPHVQMNGGFIDKYIGDAIMALFPRHPGEALDSAIAMQHEMIRFNQDRVAKGFQEIRA
                     GIGIHCGHLMLGTIGAADRMEGTVISDAVNLAARMEGLTKFYGAGILITEHVMMRLPD
                     PTRYLLRTLERVRVKGKSSAVSVIEVLDGYANCELYSASRPEFDRGMHSYLNREFKDA
                     ADSFHAVLDQNPGDGASKILLNRAIEHQNRGVGADWEGIIDMDAK"
     gene            4298..>4910
                     /locus_tag="PKK76_11410"
     CDS             4298..>4910
                     /locus_tag="PKK76_11410"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNL69127.1"
                     /translation="MRSEFALRALSPPGYHRANFMNESRKTSLTFGLFFAGTFVFSIP
                     ALFFYDPIFKNADYIRGGGFDPIISVGAIFEIFLAICNTATAIVIFPVTKRTSESGAL
                     AYVAVRIIESLIILTGVMSLMSIVTLRATATASQDPTVLVMMGKSLLAFHDWSFLLGP
                     QFCAGLGNGLVLGLLMLRSGLLPRPMALLGVIGGPLAFLGGVLV"
BASE COUNT         1183 a         1292 c         1187 g         1248 t
ORIGIN      
        1 ccggcatcga cgggattgga tcgcttgtgc agagtgtgat cgccgccctt catcggtgat
       61 ccattcgcaa accgcctgga gtttctcgca tggacgcagc cgtagactag agacaagaaa
      121 tggaagtgac agatggcaga caattcgcag tctacatgcg cactgtgcgc aagcgaactc
      181 tggatgtagt acaaaaagtg ccgatcgatc gcagccagtg gagactatcg ccagatagca
      241 tgtctgtggt ggatatcatc cttcatattg cttccgtgga acaagcatta tggggattgg
      301 acatggcaac cggaacaccc ggaaaactcc gtgaatttga aaaagattca atgaatcttt
      361 cagatgcgat agccaccatg gcgaatgtgc gcaaaggatc ctctgaattc tggtcctctc
      421 tcaccacgac tcaacttgaa caggacatcc tgactccaac cggacattct cttccgctca
      481 agcgctggct ggtgttggcc gcagaacatg aaattcacca tcgttcgttt atccacgcat
      541 atcgcaaaat atggggattg gcatcttctc ccatctatgg tctgacgtta gacgaacttc
      601 gtcaacatct tgaacccaaa cccgcatccg attcatgatc tggccaatga cctgctacgt
      661 gtgcaacgca gaccctttgc tttctctacg gtccgcgttg cacgcaatga caattaacgg
      721 cccccacgcc cccagacagg cgcacctgtt tccgtgcgca atttaagtgt ctgactacct
      781 ttcgtaacct gtcgagcgat gattgcattg cctgttctgg acccaatgac cgtaattgaa
      841 tcacccttca gcactttgac cgattgcttc tcaaggtaaa aggttggtcc aagcatcacc
      901 acgagcgttt cgttgcctgc ggacatggca agatgtatgc cctcccgcat atcgccgtgc
      961 ggtactctgg taatttctgt cactgtcccg gataccgtca cttctttgga cgcatcataa
     1021 ttcttgccca tgcccgatcc tggttgtccg tacagggaaa acgcaaacac cagtgcaagg
     1081 attgaataca gattcctgtt catcctaacc tccatgactt ttcgtcttta taataaaagc
     1141 aggaagaact ggcgccagcc ccgagaaaga tcagatatta cccgtgataa ttatcaggat
     1201 ttcctcatga actacatcaa gcctctcatc gccatcctgg ttctatcatt cgcgcgctgt
     1261 ggctacgaag acattccaaa tccgcgaaca ggaaaaacag agagacaata ccagcgagaa
     1321 aaggatcagc gaaatgcgcg caagcgaatc gagttctgca caatcacggt tctggtctgt
     1381 cagagcatga cacctccggg cggcaccgct ccgggagggt gcaatttcac cttgtcttat
     1441 gtgtgcacgg actatacaaa caccatctaa cgcaggcaaa tctgactgga cgaattgaat
     1501 gacctgctct aacataacac acgttagtcg cccttgatca tgcattcatg gcgctaaaag
     1561 gagaaagaaa tgtccaacac gggaacagtc cgtctgcaca gagtactgcg agcaccaccc
     1621 gatcgcgtct acaaagcatt tgtagacaaa caggcgctcg agcgctggct tccgccttac
     1681 ggatttacgg gagcgattgg cgaaatggac gttcgggtgg gtagcggata caaaatgtct
     1741 ttcaccaatt tttcgggtgg aggcagtcat tctttctctg tgaaatttgt agaactcgtg
     1801 ccggcagagc gaattgtgca cacggatcga tttgatgacc caaacctccc gggtgaaatc
     1861 acagtgacca tccagctgaa gaaggtttct tgcggaacgg atctttctat tgtacaggaa
     1921 ggcataccag cagtgattcc ggaagaaatg tgctatctcg gatggcagga gtctctcgat
     1981 caactgactc gactcgtgga accggaaata tccgcgaact gatagcgccc tttgtttcaa
     2041 cagaactgaa tgaatcacca aaaggtagaa agacccggca agatagcgtt ctgcgtggga
     2101 tggtcaattt tctcaatgct tgcgtccgtt tcctcccccg ttcgagcgca gtctggatta
     2161 tccatccaga ccattcccga tctttccgtg atgactagtc tcgacaccac gacgcagaat
     2221 tcctggtggt tagcaaccga gcccatagat gcagatagag aaaccaagga cgccgtggcc
     2281 atctgcaatc agaaaatgat cgaccggccc gcgttgcact ggaagcaaat caccgtgcca
     2341 gggatcattg aaagacaaac gaattttcgg ggatccacat tctggtatcg aagattattt
     2401 cgtttgtccg gggcgccgga agcctctgta gcggtccgcc tcggcaggat aaatgatcga
     2461 gaccaaacct atttaaacgg tgtattgatc ggggcaacag gcgaatggga ttccacaatt
     2521 ccggcagggt atgacagaac gcggatatat ggcattccgg ataacctctt gaaccgcaga
     2581 ggctgcaatg tgcttttgat tcacgttaag cgctatttcg cagaatcgtc gggaatagcc
     2641 acgggcgtca ccgtgattgg tccggcttct tctataacgc atgaatattt cctggagatg
     2701 attcgcacat cctggatgct cggcgcctac cctgccctgg gacttctctt tctcatcata
     2761 tatgcggtgc gacgcaaaca aaccgaatac ctgtattttg ggctatttac gattcttctc
     2821 accctttatg aattgtttcg attcgacgtg aaattcgaat tgggcatttc tttctttgcg
     2881 acaaagcgca ttgaatatgt gttattgtat tgtttgtttc cgatctttac agcgttcctt
     2941 gatacctatt ttgacgcggc cagtctgttt cgcacccgga gcattcgtcg cgtcattcta
     3001 gcagcgaatt tgtatttgat ctgcttcatt ctacccacgt tgctttcgaa cgacgtcgac
     3061 ttctggtgga ccctgcaaag ccactttatt cagccagtct ggccagtttt atttggctat
     3121 tgcctgctca ttctcgccct ccagagtatg agaggtcgaa aagatgcgct tctactcgtt
     3181 cctgccactc tgttgctaat ggcggcggtg gtcctcgata tccttcatgc ctggaacgtg
     3241 gtcatccatc ttccaccgtt aaccatatac gcctttagca tatttatttt gtcgattgcc
     3301 ttcctcctgg cgcagcgttt tgcgcaatca ttttcggctg tggaaactac gatgagtaat
     3361 ctgaacgcga ccaacgcggc ctacagccga tttgtcccgg ccgagttcct tcagatcctg
     3421 ggcaagcgcg aaatcacaga agtggctctt ggcgatcaaa cactacgaga gatgactatc
     3481 ttgttctcag atattcgttc cttcacagtg atgtccgagg ccatgacacc ggaagagaat
     3541 ttcaacttcc tcaactccta cctcaaacga atgcagccgc atgtgcaaat gaacggtggt
     3601 ttcatagaca aatatattgg cgacgccatc atggcgctgt tcccgcgaca tcccggagaa
     3661 gcgcttgatt ccgcaatagc aatgcaacac gaaatgattc gattcaatca agatcgagtc
     3721 gccaaaggat tccaggaaat tcgggccggg attggtattc attgcgggca tttgatgctc
     3781 ggcaccattg gcgctgcaga tcgaatggaa ggcaccgtca tatcggacgc agtaaatcta
     3841 gcagctcgca tggaaggact caccaagttt tacggagctg gaatcttgat cacggaacac
     3901 gtaatgatgc gcctgcctga tcctacacgg tatcttcttc gtaccctgga acgcgtccgc
     3961 gtaaagggaa agagctccgc ggtatccgtc attgaagtgt tggacgggta tgctaactgc
     4021 gagctgtatt ctgcttcgcg tccagaattc gatcgcggga tgcactccta cttaaatcgt
     4081 gaatttaagg acgcagcgga ttcatttcac gctgtactcg atcaaaatcc gggagatggc
     4141 gcaagtaaaa tcctgctcaa tcgagccatc gagcaccaga atcgtggcgt gggagcagac
     4201 tgggaaggga tcattgatat ggacgcaaaa tgaaatcctg agcatatcag atggttgcgt
     4261 ggaagtgaag atcagtccgc tctagaaaaa ttcccgaatg cgaagcgaat ttgcattgcg
     4321 cgcgctcagc cccccggggt atcatcgcgc gaactttatg aatgaatcta gaaagacctc
     4381 tctcaccttc ggactgtttt tcgccggcac tttcgttttc tccatccctg ctctattctt
     4441 ctacgatcca atcttcaaaa acgcagacta catccgcggc ggggggtttg atcccatcat
     4501 ctccgtcggt gctattttcg aaatctttct ggctatctgc aacacggcca cggccatcgt
     4561 gatctttcct gttacaaagc gcacgagtga atcgggcgct ctggcgtacg ttgctgtgcg
     4621 aatcatagaa tcgttgatca ttctcacagg agtcatgagc ctcatgtcga ttgtaactct
     4681 gcgggccact gcaactgcga gtcaggaccc gaccgtgctt gtgatgatgg gaaagtctct
     4741 cctcgctttc cacgactggt ctttcctact tggcccgcaa ttttgcgcag gtctgggcaa
     4801 cggcctcgtg cttggcttac tgatgctgcg atccggtctc ctccctcgcc ccatggcgct
     4861 cctcggtgtc attggaggtc cgcttgcatt ccttgggggc gtgcttgtat
//
LOCUS       DAORFF010000271         4905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9728_length_4905_cov_3.342680, whole genome shotgun sequence.
ACCESSION   DAORFF010000271 DAORFF010000000
VERSION     DAORFF010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9728_length_4905_cov_3.342680"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PKK76_11415"
     CDS             <1..1085
                     /locus_tag="PKK76_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69128.1"
                     /translation="AFDKVPAARHNNYEGVSQDLVERLRSSSQARTKSSDDFQKIFRV
                     TDLDQLPKDRFLRADHYPASNERRIDLFEPKIRDLKGGYVGVGTDQNFTFIAWAKSEY
                     AYLMDFDEVVVAVNKIHLYFIEISPTFAEFKNLWDRRRQNESFEIVRRRFEKTPEWPV
                     IQMAWKTAHRGSHDVPERLADIALMERNFGLKTFSNDPVQYNYIRDMIQRGRLIAVGG
                     DLTGTKSMHTIAKAARQLGIPIRVLYMSNAEEYFRYPETFRSNILDLPVDASSLVVRT
                     ATSGAKYEFGYPPGEKFDDVPFHYNIQPVQNLQEWMKFRRYQSILTIVRARAPLSQGF
                     SIVQKTPLEAGLKESGNIVAKPPGEW"
     gene            1075..2214
                     /locus_tag="PKK76_11420"
     CDS             1075..2214
                     /locus_tag="PKK76_11420"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNL69129.1"
                     /translation="MNGESVKRFFFSRYAVFVFALAGCGALPFFGSPDPEVTFAVVGD
                     IMGHEAQIRSAMDPATGKYDYSPVFSYVKEQISKADLAVGNLELTLPGKPELYSGYPQ
                     FGSPDALPAALKEIGFDLLVTANNHSMDKSELGVVRTIDVLKSMGFLQTGTFTKAEYE
                     SMRILEVERNGIRMIFLNYTYDTNGIAVTPGVVVNVIEKDRILKDFELARARKPDIII
                     VCYHFGKEYEFVPNAYQKEWVDLAFQEGAQIVLGGHPHTLQPFERRVSADRFGKSAER
                     FVIWSLGNFVSNMQRRYVDGGIILNFKIVKAKEGMLIRGIDYTPVWVHTPIEKGRLKF
                     YVLPVREHLADSSMDKISLARMKQFLEDTDKILGDSEKAVKDPEK"
     gene            2246..2920
                     /locus_tag="PKK76_11425"
     CDS             2246..2920
                     /locus_tag="PKK76_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNL69130.1"
                     /translation="MKSLANKEQAKNLARFFKTGKGDYGYGDRFLGITVPRTRMVARA
                     ALDLPVAEIQKLLRSPFHEIRLLALIIMVLKYPRKPEEQKAIYDLYMRDRAYINNWDL
                     VDLSAHHIVGPYLEHRDRSILRTLAKSKVLWDRRIAILSCFYFIKNRDFALAFELADV
                     LISDTHDLMHKAVGWMLREIGNKDPQAERAFLAERYKKMPRTMLRYAIEKFPEKERKG
                     YLAGLQ"
     gene            complement(2922..3545)
                     /locus_tag="PKK76_11430"
     CDS             complement(2922..3545)
                     /locus_tag="PKK76_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNL69131.1"
                     /translation="MSQREYKFVIAASGASGAIYGRELLRAIASSVPGESSLIVSAAA
                     LRVYNQELKGTLRTPEEYLDANLNGIASDKRVHSFRILDHADIGAKAASGSTPSDGMI
                     ICPCSMKTLAGIAHGYTSNLIERAADVCLKERRRLVVVPRETPYSLIHLRNMTQLTEA
                     GGIVLPGSPGFYQNPRTLDDLGRFIAGRILSLFDIHHSLYEGWEGED"
     gene            complement(3542..4144)
                     /locus_tag="PKK76_11435"
     CDS             complement(3542..4144)
                     /locus_tag="PKK76_11435"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNL69132.1"
                     /translation="MSTVLFPGLPLPLHIFEPRYQLMIQRVISRKEPFIVALIKSGQE
                     ALGDVAEPYEIGTTASIKQVENLENGRMNLLAIGVDRVKIHSTSRALPYLEGEMEVME
                     YPNEDLSQCAGSLRTVVQGFLSLVAQSRKIPRIELPEDPRELIFLCATILPVSGPVRQ
                     AFLETPKLSLLSQDLQARYARQIALLKAVIDGKSDEAYLN"
     gene            complement(4141..>4905)
                     /locus_tag="PKK76_11440"
     CDS             complement(4141..>4905)
                     /locus_tag="PKK76_11440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HNL69133.1"
                     /translation="AFYRMLVLVIVCMVSLRSAAMGFNRLADRNIDAVNPRTRMREIP
                     SGALSVSAVRSFVIISLVLFVVAAFAISTTCGLLSPIAAGLVLGYSYTKRFTWLCHFV
                     LGTAIGQAPAATALAMTGSIPLSALAWSGGLALYIGGFDILYACQDAEFDRSQKLHSI
                     PSRFGIPTALWIARISHILALCLFAFAAISSQTGLVFFAFLFCTGILFLVEHALVRPN
                     RLEKIPIAFFNVNASISTVLFAGLLVDLWIRIPFFH"
BASE COUNT         1286 a         1162 c         1295 g         1162 t
ORIGIN      
        1 tggccttcga caaggtgccg gccgctcgac ataacaacta cgagggcgtc agccaggatt
       61 tggtcgagcg tctgcgttcg agctcacagg ctcgtacgaa gagctcggac gatttccaga
      121 agatttttag agtcacggac ctcgatcagc ttccgaaaga tcgttttttg cgagcggatc
      181 actatcctgc atccaacgag cgccgcattg atctatttga gccaaaaatc cgcgatctaa
      241 aaggcggata tgtaggggtt gggacagacc agaattttac attcattgcc tgggctaaga
      301 gcgaatatgc ctacctcatg gacttcgacg aagtggtggt tgcggtgaac aagattcatc
      361 tctatttcat tgagatcagc cccacgtttg cggagttcaa gaatctatgg gatcgcagac
      421 gccagaatga gagctttgaa attgtgcgaa gaaggttcga gaaaactcca gagtggccgg
      481 tcattcaaat ggcctggaag acagcgcatc gcggaagcca tgatgttcca gaaaggctgg
      541 cagacattgc tttgatggag agaaatttcg gactgaagac tttttcaaac gatccagttc
      601 aatacaatta cattcgtgat atgatccagc gcggcagact gatcgcagtc ggcggagatc
      661 ttacaggaac aaagagcatg cacacgattg caaaagcagc gaggcaactt ggaattccaa
      721 ttcgcgtgct ctatatgtcc aatgcggaag aatatttccg ttatccggaa acgttcagat
      781 caaacatcct ggatcttcca gtggatgcgt cgagtcttgt agtgcgcact gcaacatccg
      841 gtgcaaagta tgagtttgga tatccgcccg gagagaagtt tgacgatgtg cctttccact
      901 ataacattca gccggtgcaa aatctccagg agtggatgaa gtttcgcaga taccagtcca
      961 ttctcacgat cgtacgggcg cgtgctcccc tttcgcaagg tttttccatt gtgcagaaaa
     1021 cgccactgga agccggtctg aaagaaagcg gcaacattgt ggcaaagcct ccaggtgaat
     1081 ggtgagtcag tgaaacgatt cttcttttca cgttatgcgg tatttgtttt tgctctggca
     1141 ggttgcggag ctctcccatt ctttggttcg cctgatccag aagtcacctt tgctgtagta
     1201 ggcgatatca tgggtcatga ggcccagatt agaagcgcca tggatccggc caccggaaag
     1261 tatgattact cgccagtgtt ctcatatgtg aaggagcaga tttctaaagc ggacctggcg
     1321 gtgggcaatc tggagctcac tctacccggt aagccagagc tctattcagg atatcctcag
     1381 tttggttcgc ccgacgcact tcccgcagct ttgaaagaaa tcggctttga tctgcttgtg
     1441 actgcaaaca atcacagcat ggataaaagc gagcttggag ttgtgcgcac aatcgacgtc
     1501 ctgaagtcta tgggtttcct gcagacaggc acattcacaa aagcagaata tgaatccatg
     1561 agaattcttg aagtagagcg aaacggaatt cgcatgattt tcctcaatta cacctacgat
     1621 acaaatggga tcgcagtgac tcctggtgtg gtggtcaatg tgatcgagaa agaccgcata
     1681 ctcaaagact ttgaactggc gcgcgcgcga aaaccggaca tcatcattgt gtgttaccac
     1741 tttggcaaag agtatgaatt tgttcctaat gcctatcaga aggaatgggt agatctagcc
     1801 tttcaagagg gcgcgcaaat tgttctggga ggacatcccc atactctcca gccattcgaa
     1861 aggcgcgtga gtgcggatcg atttggaaag agtgcagaga gattcgtaat ctggtctctg
     1921 ggcaatttcg tctccaacat gcagcgcaga tatgtggatg gcggaatcat tttgaatttc
     1981 aagattgtga aggcaaaaga aggaatgctc attcgaggca ttgattacac tccagtctgg
     2041 gttcacacac ccatcgaaaa gggcaggctc aagttctacg ttctacctgt tcgcgaacat
     2101 ctggcagatt ccagcatgga caagatctcc cttgccagaa tgaaacaatt cctggaagat
     2161 acggacaaga ttcttggcga ctcagaaaaa gcagtcaaag acccggaaaa atagaccggt
     2221 tacggctctt tccctcgaga gagagatgaa gtctctggca aataaggagc aggcaaagaa
     2281 ccttgccagg ttttttaaga cgggcaaagg ggactacggt tacggagatc gatttctcgg
     2341 gatcacagtg ccccgcacca gaatggtggc acgcgctgct ctcgatctcc ccgtagctga
     2401 gatccagaag ctcttgcgtt ctccttttca tgaaatcaga ctgctggccc tgattataat
     2461 ggttctaaag tatccgcgca agccagaaga gcagaaggcc atttacgacc tctacatgag
     2521 ggaccgggct tacatcaata actgggatct cgtggatttg tctgcccatc acattgttgg
     2581 tccttatctg gagcatcgtg atcggtctat ccttcgtact ctcgcaaaat ctaaagtact
     2641 ctgggatcgc agaattgcga ttctttcctg cttctatttc attaagaatc gggactttgc
     2701 tcttgcgttt gagctcgcgg atgttctgat ttcagacact catgatttga tgcacaaggc
     2761 agttggctgg atgctgcgcg aaattggaaa caaggatcct caggcggaaa gggcgtttct
     2821 ggcagagcgt tacaagaaga tgcccagaac catgcttcga tacgcgatag aaaaatttcc
     2881 agagaaggaa cgcaaaggct acctggctgg cctgcaatga ttcaatcttc tccttcccat
     2941 ccttcgtaaa ggctgtgatg gatatcaaag agagaaagaa tcctgccggc aatgaatcga
     3001 ccaagatcgt ctaacgtcct cggattttga taaaaacccg ggcttcctgg aagaacaatc
     3061 ccgcctgcct cagtgagctg tgtcatattg cgcagatgaa tcaggctgta aggcgtctcg
     3121 cgcggaacga caaccaggcg tctgcgttct ttgaggcata catctgcggc gcgttcaatc
     3181 agatttgaag tgtatccgtg cgcaattccg gccagagtct tcatggaaca gggacagatg
     3241 atcatgccgt ctgatggagt agaaccgctc gcagcttttg cgccaatgtc cgcgtgatct
     3301 agaatgcgaa aagagtgaac tcgtttgtcc gaggctatcc cattgaggtt tgcatccagg
     3361 tattcctcgg gcgtgcgaag agtgcccttc agttcctgat tgtagacgcg aagtgcggct
     3421 gcgctgacta tgagagagct ttctcctggc acagagctcg cgatggctcg gagaagttct
     3481 ctgccgtaga tggcaccgct tgctccgctc gctgcaatga cgaatttgta ttctctctgg
     3541 ctcaattcag atatgcctca tcgctttttc cgtcgatgac tgctttgagc aatgcaattt
     3601 gtctggcgta gcgcgcctgc aaatcctgcg agagcaagga aagttttggc gtttcgagga
     3661 atgcctggcg aaccggaccg gatacgggta gtatggtggc gcagaggaaa atcaattcac
     3721 gaggatcctc cggcagctct atgcgcggga tctttctgga ctgggccacg agagacagga
     3781 atccctgaac tacggtgcga agagaacccg cgcactgaga aagatcttcg ttaggatact
     3841 ccatgacttc catttctcct tccaggtagg gcaaagcgcg cgaagtagaa tgaatcttca
     3901 ctcgatccac acctatggcc agaagattca ttctgccatt ctccaggttt tccacctgct
     3961 tgatggaagc agtggttcca atttcgtacg gttctgcaac gtcgcctaaa gcttcctgtc
     4021 cgcttttgat gagtgcaaca atgaatggtt ccttgcgaga aatcactctc tgaatcatca
     4081 gctggtagcg cggttcaaaa atgtgaagtg gaagtggcaa ccctgggaag aggaccgtac
     4141 tcaatggaaa aacgggatgc gaatccatag atctaccagc aaacccgcga agagcaccgt
     4201 gctgatggaa gcgttgacat tgaaaaatgc aattgggatc ttctccagcc tgtttgggcg
     4261 cacgagtgcg tgctctacta agaataggat gccagtgcaa aagaggaaag caaaaaagac
     4321 aaggcccgtt tgtgaagaaa tcgccgcaaa tgcaaaaaga cagagagcaa gtatgtgtga
     4381 gatgcgagca atccagagtg cggttggaat tccaaatcta gatggaatgg aatgcagttt
     4441 ttgtgatctg tcaaactctg catcctggca ggcgtagaga atgtcaaacc ctccaatgta
     4501 aagagcaagg ccgccgctcc atgcaagagc agacagcgga atgcttcctg tcatggcaag
     4561 tgcagtggcg gccggcgcct ggcctatggc cgtgcccagt acaaagtgac agagccaggt
     4621 gaagcgcttt gtgtaggagt agcccagtac caggcctgcg gcgatggggg acagaagtcc
     4681 gcaggtagtt gagatggcaa aagcagccac cacgaacaat actaaagaaa ttataacaaa
     4741 ggatcgaacc gcggacacac tgagtgctcc ggatggaatc tcgcgcatcc tggtcctggg
     4801 attcaccgcg tcaatgttgc gatctgcaag gcggttaaat cccatggccg cgctgcgcag
     4861 gctcaccatg cagacaatca ccagcacgag catcctgtaa aaagc
//
LOCUS       DAORFF010000272         4902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9737_length_4902_cov_3.475346, whole genome shotgun sequence.
ACCESSION   DAORFF010000272 DAORFF010000000
VERSION     DAORFF010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9737_length_4902_cov_3.475346"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1156
                     /locus_tag="PKK76_11445"
     CDS             104..1156
                     /locus_tag="PKK76_11445"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNL69134.1"
                     /translation="MTLRTIRLILFITLIPEVLILGFFVYARIQSPPKAGRMIANALG
                     IPHPAVIAHRGASYLAPEETRAAYLIARDLGADYLEGDVQRTKDGILVLLHDDTLERT
                     SNVAEVFPGRQKDTIDRFTYAELLKLDLGSWFNSANPDRANDRFKGLKIQTVEDLLAI
                     AEGGNHRPGVYLETKSPERYPGVEKDLVDLLTKRGWIGRDRVCADTPALIDESVIRPV
                     RVGCGKAAVIFQSFSAESVQVLKNIAATVPRVYLVDEEMGARDGWPALVEKARSLDAG
                     MGPVGYLSYPWHTGPAHRAGRLVHSYTINKKWQLWLMNQFGSDGVFTDRSEMALAHFD
                     RVPPLDTKKLIAESVF"
     gene            complement(1191..2429)
                     /locus_tag="PKK76_11450"
     CDS             complement(1191..2429)
                     /locus_tag="PKK76_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69135.1"
                     /translation="MTLDLQLPRNESQNIPRLLNGSAGQVLSWNLLYSDPQTSYYRIE
                     LFGTSAPEELEAHILEGCPQARVKIKDDTAHGSDALLAIGPARSLEFRKAFHRTSPEV
                     VENSASLLLKNREDARELPMVKERALFISDGGALRVKNQALLALERDGYLACKFSGMQ
                     AIPLLLDARNEEEFIKSVMALAGNVSVIRLSMLQRTMSRQIVERITEQASIPVIHAEY
                     LESSATIAAIVCNALQYHSMTLDEKVVAIVGLGPCALGLSRILSRMGASKLYGVDTEY
                     ALNSRFEKDGGTATSLDFALERSDVVVISPGTQVLIDPKRLRENQLVLSFAPSAIDMS
                     ALKPEVSRSVHLCEEPHPIYALPGLLAGVHAGAKADIEISIRIAEVLVGRCGDNRFLP
                     IPSESLVRAEAEAVSLTSRR"
     gene            complement(2479..3438)
                     /locus_tag="PKK76_11455"
     CDS             complement(2479..3438)
                     /locus_tag="PKK76_11455"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNL69136.1"
                     /translation="MIGRPTVAVRRHLLLLLVLATPLWSQAPRQPVIAILPFQAKDSA
                     RGEELASRLTAEIVARQAFPVAERAQLKKALEELAKGQTGLFQPENAPQLGRMIGADY
                     LILGEILDSTDKGGVRASMRVVRAETGIVIGAAVTGGEADAVSRDLSLSAIKTVSIHA
                     MLENPDSPYSVLLLLDKGKNPEYKLGETLRLKFKVLKHDQTASEKVYLSIYSINADGV
                     MTLIYPNRFSGQEGVYVGREYTFPDPRDDFEWKLVPPAGAETLQAIVSNKPVRLANER
                     ATEDFPVLGVKESPALYRGIQTQLKEEKRGNWAAERITYILKP"
     gene            complement(3410..3868)
                     /locus_tag="PKK76_11460"
     CDS             complement(3410..3868)
                     /locus_tag="PKK76_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69137.1"
                     /translation="MTNRTYMRHFLPLFFLLATAVAAQSEKGIDVVQRANGVFIYRYD
                     ETLEQIDPRKDFKAKKQITEQSVIADLKRLLKDNTDYTPNFKARCMPVWDAGIEFRTP
                     TETRMYLFSFRCNTVKSVEDNTFKDFTPQRTELYGLLMYEINDRTSYSGR"
     gene            complement(3928..>4902)
                     /locus_tag="PKK76_11465"
     CDS             complement(3928..>4902)
                     /locus_tag="PKK76_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNL69138.1"
                     /translation="PPNTKRDLYQYDQNITLNNLALTNPFGSGNQIFNPSQRVLRESQ
                     DKYFYYDRIRPFINISTEDFIGDTNFKWFAGVRGQRYKIQSYQGDYDNGNTVANIKTL
                     IDNERPYGYDATQDRKYVNGVRGAIAYDSRPRDRESNPNGGIFTDIHVEGVGKGTGSH
                     YSFTRTTVTWRQYFDVAPSFFNSFDKELVIGYRLLGQETSGDVPFFEAGRIYTMRESA
                     EGLGGNGGVRGYSANQFVDKDMVIGNLEMRLTTFKISALGGMDFVLLGYHDEGRVGAS
                     RREIDMDNWHKANGGGVRIVWQKNTIINISYGRSKYGANGNFSFNHLF"
BASE COUNT         1110 a         1184 c         1308 g         1300 t
ORIGIN      
        1 ccggaaaaaa cccggagaat acagttcctt cgcggactcg agaagaagta acgcctgcga
       61 gaaaagtgag ttacaggcgg atctgcatct gaagattcga tccatgaccc ttcgcacgat
      121 tcgcctgatc ttgttcatca ctctgattcc ggaagttttg attcttgggt tctttgttta
      181 tgcgagaatc cagagtccgc caaaggccgg acgaatgatt gcaaatgcgc tgggaattcc
      241 gcacccggcc gtgattgcgc acagaggcgc gtcctatctg gcacccgagg aaaccagagc
      301 ggcttacctg attgcccgtg atctgggtgc tgattacctg gaaggggatg tgcagcggac
      361 caaagacgga attctggttc tactccatga tgatacttta gaacgaacaa gtaatgttgc
      421 agaggtgttt ccgggaagac agaaggacac gatagatcgt ttcacatatg cggagctact
      481 caaactggac ctcggttcgt ggttcaattc cgcaaacccg gaccgcgcga atgatcgttt
      541 caagggattg aagatacaaa ctgtagaaga tcttcttgcg atcgcggagg gtggaaatca
      601 caggccaggc gtatatttag aaaccaaatc gccagaacgc tatcctggag tggaaaagga
      661 tctggtggat cttcttacga agagaggctg gattggccgt gaccgcgttt gcgcagatac
      721 tcccgccctc attgatgagt ctgtgattcg ccctgtgaga gtgggttgcg gcaaagctgc
      781 tgtgatcttc cagtcgttct ccgcggaaag cgtgcaggtc ctcaagaaca tcgcagcgac
      841 cgttcctcga gtctatctgg tggatgagga aatgggggct cgcgatggct ggcctgcact
      901 cgtggaaaaa gctcgctctc tggacgcagg gatgggcccg gttggttatc ttagctatcc
      961 gtggcatacg ggtcctgcac atcgcgcggg aaggctggtt cattcgtata cgatcaacaa
     1021 gaaatggcaa ttatggttga tgaatcaatt cggaagcgac ggtgtcttca cagatcgctc
     1081 tgagatggct cttgctcatt ttgatcgtgt tccgcctctg gacacgaaga agctgattgc
     1141 tgaaagcgta ttttagttta gatactttag ttttttcgcg gcgcccggtc ttatcgtctg
     1201 gaggtgaggc tcacagcttc tgcttccgcg cgcacgagtg attccgaagg gatgggaagg
     1261 aatcgattgt ctccgcatcg acccacgagc acttccgcaa ttcgaattga aatttcgatg
     1321 tccgcttttg cacctgcgtg cactcccgca agaagtccag ggagcgcgta aatggggtgc
     1381 ggttcttcgc aaagatgaac tgatctggaa acctctggtt tcagtgcgct catatcaatg
     1441 gcagacggag cgaaagaaag gaccagttga ttttcgcgca gtctctttgg gtcgatcaat
     1501 acctgagttc ccgggctaat gaccaccacg tcagatcgtt cgagcgcgaa atcgagggaa
     1561 gtggccgttc cgccgtcttt ctcaaatctc gaattcagtg catattccgt gtccacgccg
     1621 tagagcttgg atgctcccat cctggatagg atacgcgaga ggcccagagc gcacggtcca
     1681 agcccgacaa tcgcaacgac tttttcgtct agagtcatgc tatgatattg aagtgcattg
     1741 catacaatgg cggctatcgt tgcagaggat tctaaatatt ctgcgtgaat gaccgggatg
     1801 gatgcctgct ctgtgattct ctccacaatc tggcgtgaca tggttctttg cagcatcgaa
     1861 aggcggatca cggacacatt tcctgcaagc gccatgactg atttgatgaa ttcttcttcg
     1921 tttctcgcat ccagaagtag tggaattgcc tgcattccgc tgaacttgca tgcgagatat
     1981 ccatctcgct caagagcaag gagtgcctgg tttttcacac gaagagcgcc gccatctgaa
     2041 atgaacaaag cgcgttcttt taccatggga agttcgcgag cgtcctctct gtttttcaag
     2101 agcagtgacg cggaattctc tacaacttca ggagatgtgc ggtggaacgc tttgcgaaat
     2161 tcaagggacc tggccggccc gatggcgaga agtgcgtctg acccatgggc agtgtcgtct
     2221 ttgattttta cgcgcgcctg ggggcagcct tcgaggatat gcgcttcgag ttcttccggt
     2281 gcagacgttc caaagagttc aattcggtag taggacgttt gaggatctga gtagagtaga
     2341 ttccatgaga ggacctgccc cgccgatcca ttcaggagtc tgggtatgtt ctgcgattca
     2401 ttgcgcggca attggagatc tagagtcaac agagacattc atacacagta tcggggagga
     2461 cctgggcgat tctgaaggct acggcttgag aatgtacgtg attctttctg cagcccagtt
     2521 tccgcgtttt tcttctttca gctgggtctg gataccgcgg tagagggccg gtgattcttt
     2581 tacacctaga acgggaaagt cctccgttgc tctttcattt gcaagtctca caggcttatt
     2641 tgaaacgatt gcctggagcg tttctgcacc ggccggcgga accagtttcc attcaaagtc
     2701 gtcacgtgga tccggaaatg tgtactcccg accaacatac accccttcct gacccgaaaa
     2761 acgattggga tagataagag tcatgacacc gtctgcgttt atagaatata tagaaagata
     2821 aactttttca gatgcggttt gatcgtgttt tagaaccttg aacttcaggc gaagcgtctc
     2881 acccagtttg tattctggat tttttccctt gtccagaagg agaagaacgc tgtaggggct
     2941 gtccggattt tccagcatcg catggataga aactgtcttt atcgcggata gagaaaggtc
     3001 tctggaaaca gcgtctgctt ctccacctgt tacggctgct ccaattacaa ttcctgtttc
     3061 cgcgcgcact acccgcatcg atgcgcgcac gccgcctttg tctgttgaat ccaggatttc
     3121 tcccaggatc aagtagtcag ctccaatcat tcgacccagc tgtggagcat tctccggttg
     3181 aaaaagtcca gtctgtccct tcgcaagttc ttccaatgct tttttcagct gagcccgctc
     3241 tgcgacgggg aaagcctgtc tggccacgat ttcagccgtg aggcgcgatg cgagctcttc
     3301 ccctctggcg ctatcttttg cctgaaaagg aagaattgct atgactggtt gccgcggagc
     3361 ctgcgaccag agcggcgtcg ccaggaccag caatagaagc aaatgtcgcc taacggccac
     3421 tgtaggacgt ccgatcattg atttcgtaca tgagtaatcc gtataattcc gttctttgcg
     3481 gcgtgaagtc tttgaaagtg ttgtcttcta cggatttgac cgtgttgcat ctgaaagaaa
     3541 acaaatacat tcgcgtttca gttggcgtgc gaaactcgat tccagcatcc caaacaggca
     3601 tgcaccgggc tttgaaattg ggcgtgtagt ctgtgttgtc tttcaggagt ctttttagat
     3661 cagcaattac actttgctct gtgatttgtt tttttgcttt gaaatctttt ctgggatcaa
     3721 tctgttctag agtctcatcg tagcggtaaa tgaaaacgcc attggctctc tgtactacgt
     3781 cgattccctt ttcagattgg gcggcaaccg ctgttgcaag tagaaagaac aagggcaaaa
     3841 aatggcgcat ataggttcga tttgtcacgg cgggaaaagg ttctagcttt ttttgcgggc
     3901 gcaggggccc gcaaaaaagc agttcgctta gaagaggtga ttgaaggaga agttgccgtt
     3961 tgctccgtac ttggagcgac cgtagctgat gttgatgatt gtatttttct gccatacaat
     4021 gcgaacaccg ccaccgtttg ccttgtgcca gttgtccatg tcgatctcgc ggcgggaggc
     4081 acccacgcgt ccttcgtcat ggtagccgag cagaacaaag tccataccac cgagtgccga
     4141 gattttgaat gtggtcagac gcatttccag gtttccaatg accatgtctt tgtctacgaa
     4201 ctggttcgca gaatagccgc gcacaccgcc gtttccgccc agaccctctg cactttcgcg
     4261 catggtgtag atacgaccgg cttcgaagaa tggaacatct cctgacgttt cctgtcccag
     4321 aaggcggtat ccaatcacga gttctttgtc aaaggaattg aagaaagaag gtgcgacgtc
     4381 aaagtactgt ctccaggtca cagtggtgcg agtgaaagag tagtggcttc ccgttccttt
     4441 gcccactccc tccacgtgaa tgtccgtgaa gataccgcca ttaggattgc tttctctgtc
     4501 gcgtggacgt gagtcatacg cgatggctcc gcgaacaccg ttgacgtatt tcctgtcttg
     4561 agtcgcatca taaccgtatg ggcgttcgtt gtcgatcagc gtcttgatgt ttgcaacagt
     4621 gttgccgttg tcatagtcgc cttgatagga ctggattttg tagcgctgcc ctctgacacc
     4681 ggcgaaccac ttgaagtttg tgtctccaat gaaatcttcc gttgagatgt tgatgaacgg
     4741 acgaatccgg tcatagtaga aatacttatc ctgactttcg cgcaaaactc tctgcgatgg
     4801 gttgaagatc tggttgcctg aaccaaacgg gtttgtaagt gcgaggttgt tcagagtgat
     4861 gttctggtca tactgataca ggtctctctt tgtgtttggc gg
//
LOCUS       DAORFF010000273         4899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9753_length_4899_cov_3.666391, whole genome shotgun sequence.
ACCESSION   DAORFF010000273 DAORFF010000000
VERSION     DAORFF010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9753_length_4899_cov_3.666391"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKK76_11470"
     CDS             <1..271
                     /locus_tag="PKK76_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA ligase (NAD(+)) LigA"
                     /protein_id="HNL69139.1"
                     /translation="LQFSQKIEKSNLQQIFEGQTWCVTGSFENFKPRDLAMAEVKKRG
                     GKVVSGVSKKTSHLLAGEGAGSKLEQAEKFGTTIVSEEEFLRLIG"
     gene            complement(278..541)
                     /locus_tag="PKK76_11475"
     CDS             complement(278..541)
                     /locus_tag="PKK76_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HNL69140.1"
                     /translation="MAIELSKEDTQSILPSIIQYMREELEVELSEMRAGFLLKYFMEE
                     IAPFAYNKGVRDAEQYMQEKVGDLTGVVYEAPLNYWKKKKKGK"
     gene            complement(560..1021)
                     /locus_tag="PKK76_11480"
     CDS             complement(560..1021)
                     /locus_tag="PKK76_11480"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNL69141.1"
                     /translation="MATRRTELRFTNYKDIIQDIQRLQQSGYEMTGKWNLGQICRHLS
                     FYWKGSLDGFTVQLPWIIRATLGRMILKSSLKETIRKPGGQTAPQSVFAAEPDDREAV
                     AEAIALLQRLDQNTAKLHPSALFGEVTNDQWKTLHCNHAGHHLSFLVPKGA"
     gene            complement(1023..1571)
                     /locus_tag="PKK76_11485"
     CDS             complement(1023..1571)
                     /locus_tag="PKK76_11485"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNL69142.1"
                     /translation="MDPKTKQEIASAMRTQASQLKEFYSSIPDNVFFVRPAKGWSAAG
                     NVRHLKKAAQGVLVGMYTPKGIIRVIFGKSKKPPRTAVQLKELYLRKLGQGAGAGLFS
                     PVNKGGSGNALKKAKLIESFSRATESLAKSLDRFSEEELDQLRLPHPILGMLAIREMI
                     NFGLFHNEHHTGIVRERMKKGN"
     gene            complement(1573..4170)
                     /gene="leuS"
                     /locus_tag="PKK76_11490"
     CDS             complement(1573..4170)
                     /gene="leuS"
                     /locus_tag="PKK76_11490"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNL69143.1"
                     /translation="MSYPFQEIESRWQNHWEKHSIFKTPEDRTRPKFYILDMFPYPSG
                     AGLHVGHPEGYTATDIVARFKRMRGFNVLHPMGWDAFGLPAERYAMKTKIHPSITTEQ
                     NITNFRRQLKALGFSYDWDREINTTDPNYYKWTQWIFLQIFNSWYDTDAGKARPIKEL
                     PIPAELKKKGKEAEKEAFINSKRLAYIAEMPVNWCPGLGTVLANEEVDEWVSKGYTVE
                     RRPMRQWMLRITAYAERLLGDLSTVEWPTGTLELQKNWIGKSTGASIKFAVKDSNHFI
                     EVFTTRPDTLFGISYMVLSPEHPLVEEVTIPAQKSAVESYREEARKKSDLERQLKGEK
                     EEKTGVFTGGYAIHPYTKESIPIWIADYVLMGYGTGAIMAVPAHDERDFAFARKFNLP
                     VKIVVEPVSGKAPAEEAFTGEGKSVNSDFISGLPSKEATLKMIERIQADGLGESRVNY
                     KLRDWLFSRQRYWGEPVPVSFTKDGRYVGEKEKNLPLRLPDCEDFQPAETGESPLAKL
                     KDWVEHEDEKLGKLRRETNTMPQWAGSSWYYLRFIEPRNKKALVSEQEEKYWMGETGV
                     DLYVGGAEHAVLHLLYARFWHKVLFDLGHVHSIEPFHKLVHQGLILGEDGQKMSKSLG
                     NVVNPDDVLVEFGADSFRLYEMFLGPLEMSKPWSTKAIGGVYRFLSRVWRLYTARGAG
                     DSDEDGENARSSAHSPLDEVLLVEPEGDRRVKVERLLHQTIRKVTEDIEKMSFNTAVS
                     ALMVFVNETTAEKKIGRDAAEKFLLLLSPFAPHIAEELWSKLGHNESIAHSAWPGFDL
                     TKTQEDEIEIVFQVNGKVRGKAKMATGSSDDQMKTAALADEAVLRAMEGKEPRKVIVV
                     KNKLVNLVV"
     gene            4182..>4899
                     /locus_tag="PKK76_11495"
     CDS             4182..>4899
                     /locus_tag="PKK76_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69144.1"
                     /translation="MEKGPWIEFFPVGRFGQADNQNSMVRGLVCVFMLVFSGALHAEG
                     GELIYAFRNYTRPDPIKMLLVGEIQSKIKAPRITEQQSPFSGYDTRLDQVTIKVRNPT
                     GLKVGQKLYIIDKNPHHEKFRNGLIVGEITVQSVLKTSFYGWVVTGTGILLRVREGLF
                     VARTQESENLVHAFDLKKKGDFYADSGAFDKAVATYQASLVADPGLPEAHAALGTLFY
                     QESKKGSHEFPSRSLSEFEQA"
BASE COUNT         1104 a         1225 c         1188 g         1382 t
ORIGIN      
        1 gcttcaattc tcgcagaaaa tcgagaaatc aaatctacag caaatttttg aagggcagac
       61 gtggtgcgtg actggcagct ttgagaattt caagccacgc gatcttgcca tggcagaagt
      121 gaagaagaga ggcgggaaag tagtttccgg tgtttcaaag aaaacttcgc atctactggc
      181 cggagaaggc gcggggagca agttagagca agcagagaag ttcggcacta cgattgtcag
      241 cgaagaagag ttcctgcgtt tgatcggctg atggccgcta tttccctttc ttcttctttt
      301 tccagtagtt gagcggggcc tcatacacga cgccggtcag atctcctacc ttttcttgca
      361 tgtactgctc cgcatcgcgt acacccttgt tgtacgcgaa tggagcgatt tcttccatga
      421 aatatttgag taagaatccg gcccgcattt cgctgagttc cacttcaagc tcttcgcgca
      481 tatattgaat gatggaagga agaatggact gcgtgtcctc tttgctcagt tcgatagcca
      541 tagggttctc cttgtaacgt taagcgccct ttggcaccag aaaactcaaa tgatgtccgg
      601 catgattgca gtgcaaggtt ttccactgat cgttggtcac ttctccgaaa agagcagatg
      661 ggtggagctt cgccgtgttt tgatccaacc tttgcagaag tgcaattgct tctgccaccg
      721 cttctctgtc atccggctct gcggcgaata cagattgagg cgcggtttgt cctcctggct
      781 tgcgaatggt ttccttcagc gagcttttga ggatcattct tcccagagtg gcgcggatga
      841 tccagggaag ttgcactgtg aatccatcca gggagccctt ccagtagaaa ctgaggtgtc
      901 tgcaaatctg tcccagattc catttgcctg tcatttcata tcctgactgc tgcaagcgct
      961 ggatgtcctg gatgatgtct ttgtaattgg taaagcgaag ctccgttctt ctggtggcca
     1021 tgttagtttc ctttcttcat tctttcgcgc acgatgccag tgtgatgctc gttgtgaaac
     1081 aaaccaaagt tgatcatttc gcggatagca agcatcccga ggatgggatg cggtaggcga
     1141 agttgatcca gttcttcttc tgaaaaacga tcgagtgatt tcgcaagtga ttctgttgcc
     1201 ctgctaaacg attcgatgag ctttgctttc ttcagcgcat ttccagaccc acccttattt
     1261 acaggagaaa acaatcctgc tccagcaccc tgtccgagtt tgcgaagata gagctccttg
     1321 agctggactg ctgtccgggg tggcttcttg cttttcccaa agatgacgcg aataataccc
     1381 tttggagtgt acattccaac gagaactccc tgcgctgctt tttttaaatg acgtacgttg
     1441 cctgctgcag accatccctt cgccggtcgt acaaaaaaga cgttatccgg aatcgatgag
     1501 tagaattctt tcaattgcga cgcctgggtt ctcatcgcgg aggcgatttc ttgttttgtc
     1561 ttaggatcca ctttacacca ccagattcac gagtttgttc ttcactacga tgactttgcg
     1621 aggttctttt ccttccatgg ctcgcaaaac cgcttcgtct gcaagtgcgg cagttttcat
     1681 ttgatcgtct gatgatccgg tcgccatctt tgctttgccg cgcactttcc cattcacttg
     1741 aaacacgatt tcgatttcgt cttcctgcgt tttcgtcaga tcaaatccag gccaggcgga
     1801 gtgtgcaatg gactcgttgt gaccaagctt actccagagt tcttctgcga tgtgaggcgc
     1861 aaaaggagaa aggagaagta gaaatttttc ggcagcatcg cgtccgattt ttttctcagc
     1921 agtggtttca tttacgaaca ccatgagagc agacactgca gtattgaaag acatcttctc
     1981 tatgtcttct gtcactttgc gaatggtctg gtgaagaagc ctctctactt ttacgcgcct
     2041 gtctccttct ggttccacaa gcagaacttc atccagaggc gaatgcgcac tcgatcttgc
     2101 attttctccg tcttcgtctg aatctcctgc accgcgcgcg gtgtagagcc tccatacgcg
     2161 cgatagaaaa cgatacactc caccgattgc tttggtagac cacggtttgc tcatctccag
     2221 tggacccagg aacatttcgt agagacggaa tgaatctgct ccaaactcaa cgagaacgtc
     2281 gtccggattt acaacattgc ccagagactt ggacattttt tgtccgtctt cgcccaggat
     2341 gagtccttga tgaacgagtt tgtgaaatgg ttctatggaa tgaacatgcc ccagatcaaa
     2401 gagcaccttg tgccagaacc tcgcgtagag caaatgaaga accgcgtgtt cagcgccacc
     2461 cacatacaga tcgactcctg tttctcccat ccagtatttc tcttcctgtt cggaaacaag
     2521 agctttctta ttgcgaggct caatgaacct caggtaatac caggagcttc ccgcccattg
     2581 aggcattgtg tttgtttcgc gacgaagttt gcccaatttt tcatcttcgt gctctaccca
     2641 gtcttttagc ttcgccagag gcgattctcc cgtctctgcg ggctggaaat cttcgcaatc
     2701 aggaaggcga agcggaaggt tcttttcctt ttctcctaca tatcttccat cttttgtaaa
     2761 cgacacaggc acaggttctc cccagtatct ctgtcttgaa aaaagccagt cgcgcaattt
     2821 gtaattcaca cgactttctc cgaggccatc tgcctggatt cgttcgatca ttttcagtgt
     2881 tgcttctttg gagggaagtc cggatatgaa atcggagttc acactctttc cctctcctgt
     2941 gaatgcttct tccgcgggtg cctttccgct cacgggttcc actacaatct tcacaggtag
     3001 attgaacttt cgcgcgaatg cgaagtctct ttcatcgtga gctggaactg ccatgatagc
     3061 gcccgtgccg tatcccatga gcacatagtc tgcgatccag atggggatgg actcttttgt
     3121 gtaagggtga atcgcataac cacccgtaaa cacacctgtc ttctcttctt tttctccttt
     3181 gagttgtcgc tcaaggtctg acttttttct ggcttcttct ctgtacgatt ctacagcgct
     3241 cttctgggcc gggatggtca cctcttctac cagaggatgt tccggggata gaaccatgta
     3301 agagatgccg aataacgtgt caggtctggt tgtgaatact tcaatgaagt ggttgctgtc
     3361 tttgaccgcg aatttgatag aagcccctgt ggatttcccg atccagtttt tttggagttc
     3421 cagagttccg gtaggccatt ccacggtact cagatcgcct aacagacgtt ctgcgtatgc
     3481 ggtgattcgc agcatccact gacgcatggg tctgcgttct acggtatagc ctttgctgac
     3541 ccattcatct acttcttcgt ttgcaagcac ggtgccaagg ccgggacacc agttcacagg
     3601 catctctgcg atgtaggcca gtcgtttcga attgatgaat gcctctttct ccgcctcttt
     3661 tcctttcttt ttcagttctg caggaatagg aagttctttg atgggacgtg ctttgcccgc
     3721 atctgtgtca taccaggaat tgaaaatctg caggaagatc cattgcgtcc atttgtaata
     3781 gtttggatcc gttgtgttga tttcgcgatc ccaatcgtat gaaaaaccca gagccttcag
     3841 ttgcctgcga aagttcgtga tgttttgctc tgtagtaatg gatggatgaa tctttgtttt
     3901 catcgcataa cgttccgctg gcaaaccaaa tgcgtcccag cccattggat gaagaacgtt
     3961 gaatcctctc attcgcttaa agcgcgccac aatgtccgtc gcagtgtatc cttccggatg
     4021 accaacgtga agccctgcac ccgaagggta ggggaacatg tccaggatgt agaatttagg
     4081 acgagttctg tcctctggag tcttgaaaat ggaatgcttt tcccagtgat tttgccagcg
     4141 ggattcgatt tcctggaaag ggtacgacat acggccagaa aatggaaaaa ggcccctgga
     4201 tcgagttttt tccggtgggc cgtttcgggc aggccgacaa tcaaaacagt atggtgcgcg
     4261 gtctcgtttg cgtctttatg ctcgtctttt cgggtgcttt gcacgcggaa ggaggagagc
     4321 tgatctacgc atttcgcaac tacactcgcc cggatccaat caagatgctc ctcgtgggag
     4381 aaatccagag taagatcaaa gcgcctcgca ttacggaaca gcaatctccc ttctccggct
     4441 acgacactcg cctggaccag gtcacgatca aggtgcgaaa cccgaccgga ctcaaggttg
     4501 ggcaaaaatt gtatataatt gataagaatc cgcaccacga gaaattcagg aacggcctga
     4561 ttgtgggcga aatcacagta cagtctgtac tgaaaacaag cttctacggc tgggttgtaa
     4621 cgggcactgg aattctactt cgcgtacgag aaggtctgtt tgtagcgcgc actcaggaat
     4681 ccgagaacct ggtgcatgcc tttgatttga aaaagaaggg cgatttctac gcagatagcg
     4741 gcgcattcga caaagcagtg gctacgtatc aggcgtcgct tgtggcggat ccaggacttc
     4801 cggaagcaca cgcagccctg ggaactcttt tttatcaaga atcaaagaaa ggaagtcatg
     4861 aatttccttc gcgatctctg tccgagttcg agcaggcat
//
LOCUS       DAORFF010000274         4879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9820_length_4879_cov_3.461443, whole genome shotgun sequence.
ACCESSION   DAORFF010000274 DAORFF010000000
VERSION     DAORFF010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9820_length_4879_cov_3.461443"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PKK76_11500"
     CDS             <1..330
                     /locus_tag="PKK76_11500"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNL69145.1"
                     /translation="GIAATPDFELVFDTIKTTRKYRNLAQDPRASLVIGCTESGITLQ
                     YEGVARELRGIEMEKYRQIYFAQWPDGTARLSWDGITYFAVRPSWIRYCNYAVEPPVL
                     TEFTFPF"
     gene            complement(367..1593)
                     /locus_tag="PKK76_11505"
     CDS             complement(367..1593)
                     /locus_tag="PKK76_11505"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNL69146.1"
                     /translation="MKLSPFAAVRPRSDLAAEVVCLPYDVMNTAEAREMAHGKPSSFL
                     HIIRSEIELPDSADPHTDAVYQLAKKNLETMLSDGRLLRESGPCLYVYRLVKDGRNQT
                     GLAALNHIDDYLQDKIKKHELTRRDKEEDRLHHIDTTGANCEPVFLAFKSGEQPEIAR
                     ILNEVADKEKPLYDVTPEDGVRHLVYSIPVGEKQDKLKSLYESLSCVYIADGHHRSAS
                     TVRVGKARREASGPGSHEWFLAVTFPDADLRILPYNRVVKDLNGLSKEAFFEKVKKSF
                     QLSEGKSNPALHTVHMYLDRQWYTLTAKEETYKGADAIGRLDVSILQNQLLDPILGIA
                     DPRTSERVAFVGGIRGEGELIKLVDSGAWAVAFSMYPTSMQELFDIADAGQIMPPKST
                     WFEPKLRSGFLVHKFS"
     gene            complement(1647..2186)
                     /locus_tag="PKK76_11510"
     CDS             complement(1647..2186)
                     /locus_tag="PKK76_11510"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNL69147.1"
                     /translation="MARTPVKKTTFLEAVAACIQHEKEVFGFYLKQAEALPEGAIKKL
                     FFQLAEDVEEHIRMISEIYSQVKGGEALPNLKMASEVQKFNGTSLQILMRRLDRITGS
                     DGKGNEIEAIALATREHEDAAAFYRKMTDKFEDKGIQVLFGQLANFQDECRLLLESYG
                     AYLTQGTPASQPSAYWDET"
     gene            complement(2252..>4879)
                     /locus_tag="PKK76_11515"
     CDS             complement(2252..>4879)
                     /locus_tag="PKK76_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNL69148.1"
                     /translation="DTSFLVWTTTPWTLPSNVALAIAPNESYALVEAVREDGSEKIIC
                     AEVLLESVLSNTKVTSHSIIKKMKGSDLAGVRYERLFPWLPTEGDKAFRVYTADFVTM
                     DTGTGIVHIAPAYGADDLVVGQTHDLPVLYAVGLDGKFVSGTPQAGVFFKDADKDLVR
                     DLKERGLVWKSERYKHEYPFGWRTGAPLLYFAKDAWYIRTTRVREHLVKNNDSINWVP
                     DHVKQGRFGNWLENNRDWALSRERYWGTPLPVWTDGENFKIIGSIAELEKLTGQKLTE
                     LDLHRPFIDRITWKEGAAKTAKAAGTAAKSQKKSGKSSKAATSGEEAKEWKRVPEVID
                     CWFDSGAMPYAQWGTSPAGKKELAKYFPADFICEAIDQTRGWFYTLLAISTMVSDETS
                     YKNVVCLGHVLDGKGEKMSKSKGNTVDPWQVFEMHGADAIRWHFLVGAPPGNSRKMGK
                     PGTDQDPIGDVFGFFNMLQNSAGFFSLYANVDGIEINEWQKNPVAGALPFKERPDIDR
                     WILSRLQVLIRDVTASMNEYDAQKAGRLVELFTDDLSNWYIRRNRRRFWKGERDAEKL
                     TGFDTLYRCLVTIARLIMPFAPFLGEELYRGLVASQSKSAPISANLAGWPEYDASFFD
                     AKILEEGDLVLQAASLGRAARAASGMKIRQPLSRIRVFAANASAKSAIEKNEDVLKEE
                     LNVKSIEFLADAQGLIDYRIKPNLPRLGKRVGQHMKAVQEYFRSIKDPSTVARAVRAG
                     SSLSLEDQGVKIELEPEDVLVEPVSAEGTAGVEGSGMTVVLDTGLTPELIQEGIVRDL
                     VRNIQEMRKKAGLEVTDRITLVMEADPEVSSAIERFAGYIEDETLAKIEKSHAGKATG
                     ESAFDLDGLSVSVKLFSLR"
BASE COUNT         1170 a         1262 c         1185 g         1262 t
ORIGIN      
        1 ggaattgctg cgacccctga ttttgaactt gtttttgata caatcaaaac aactcgcaaa
       61 tatagaaatc tggcgcaaga tcccagggca tccctcgtga taggctgcac tgaatcggga
      121 atcaccttac agtacgaagg tgtagcacgt gaactcaggg gaattgaaat ggagaagtac
      181 cgccagattt actttgcaca gtggccggac ggtacagcca ggttgagctg ggacggaatc
      241 acgtacttcg cggtcagacc atcgtggata cgctattgca attacgcagt ggagccgccc
      301 gttctaacgg aatttacatt tccgttttga acgggctgtg gttccagatc ctgacctgag
      361 gagaaatcag gagaacttgt gcacgagaaa accagacctg agtttgggtt caaaccaggt
      421 ggacttggga ggcatgatct gtcctgcgtc tgcaatatca aacagctctt gcatggaagt
      481 tggatacatg ctgaacgcaa ccgcccaggc accgctgtct acaagcttga tgagttcccc
      541 ttctccgcga atccctccga caaaagcaac gcgttctgaa gtgcgtggat ctgcgattcc
      601 aagaattgga tcgaggagtt gattttgcag aatgctcacg tcgagcctgc caatggcatc
      661 cgcgcctttg tacgtttctt ctttcgcagt gagcgtatac cattggcggt ccaggtacat
      721 atggactgta tgcaaagcag gattgctttt tccttcgctc agctgaaaag acttcttgac
      781 tttttcgaag aacgcttcct ttgaaagacc gttcagatct ttcactacgc gattgtatgg
      841 gagaattctt agatccgcat ccgggaaagt aactgcaaga aaccattcat gggatccggg
      901 tcctgatgct tcgcgacgag ctttcccaac gcgaacggtg gaggcgcttc tgtgatgacc
      961 atctgcgatg tacacacacg aaagactttc atagagtgac ttgagtttgt cttgtttttc
     1021 tcctacgggg atggagtaga ccagatggcg cacgccatct tccggagtta cgtcgtaaag
     1081 tggcttctct ttgtctgcca cctcatttag gatgcgagca atctctggct gctcacccga
     1141 tttgaaagcc agaaatacag gctcgcaatt cgcgcctgtt gtgtctatgt gatgaaggcg
     1201 atcctcttct ttgtcgcgac gagtcagctc atgctttttg attttgtctt gaaggtagtc
     1261 gtctatatgg ttgagggcag ctagacccgt ctgattgcgg ccgtctttca cgagacgata
     1321 cacatacagg cacggaccgc tttcgcgcag aagtcttcca tcagaaagca tggtttccag
     1381 gttcttcttc gcaagctgat acactgcatc cgtatgagga tccgcactat caggtagttc
     1441 aatttcagat cgaatgatat gaagaaaaga agaaggctta ccgtgagcca tctcacgggc
     1501 ctccgcagtg ttcatcacat cgtagggaag acagaccacc tccgccgcaa gatcagatct
     1561 cgggcggaca gcagcaaaag gggaaagttt catgagtcgc aggttcctat ctaagattta
     1621 gctggcgcct gcgaagaaag gacggatcag gtttcatccc agtaagcact cggctggctg
     1681 gccggtgtgc cctgggtaag gtatgctccg taggactcga gaagcagtct gcattcgtcc
     1741 tgaaaattcg caagctgacc aaacaatacc tgaattcctt tgtcctcaaa cttgtctgtc
     1801 attttgcgat agaacgccgc cgcatcttca tgctctcgcg tggccagagc gatggcttct
     1861 atttcattgc cctttccgtc acttccggta attcggtcca gacgacgcat caggatctgc
     1921 agagaagtac cgttgaattt ttgaacttca gatgccattt tcaaattcgg gagcgcttct
     1981 ccacctttca cctgggaata gatttcagag atcatgcgga tgtgctcttc tacatcctct
     2041 gcgagttgaa aaaagagctt cttgattgca ccttctggca acgcttccgc ctgcttgagg
     2101 taaaaaccga atacctcttt ttcgtgctgg atgcacgctg ctacggcctc aaggaacgtg
     2161 gtcttcttaa cgggtgttct agccattggt agatggaacg gttcgaagag tattctggca
     2221 aatgaaattt cataggccag agagcattca attacctcaa actgaaaagt ttgacagaaa
     2281 cagatagacc gtccagatca aaggcagatt cgccggtagc tttccccgca tgcgattttt
     2341 cgattttcgc aagagtctcg tcttcaatgt aaccggcaaa gcgctctatc gcactggaga
     2401 cttccggatc tgcttccata accagagtga ttctgtctgt aacctcaaga cccgccttct
     2461 tgcgcatctc ctgaatgttt cgaaccagat ctctgacgat tccctcctgg atgagttcag
     2521 gagtcaatcc ggtatcgagg acgacagtca tgccgcttcc ctctactcct gctgtccctt
     2581 ctgcagacac tggctcaaca agaacgtcct ctggttccag ttctatcttc acgccctgat
     2641 cttccaggga gagagatgaa ccggctctta cggcgcgagc aaccgtggat ggatccttga
     2701 tacttctaaa gtattcctgg acggccttca tgtgctggcc aacgcgcttg cccagccgcg
     2761 gaagatttgg tttgatacga tagtcgatga gaccctgcgc gtccgccaga aactcaatgc
     2821 tcttaacatt cagttcttct ttgagaacgt cttcgttttt ctcgatcgca gacttagcgc
     2881 ttgcatttgc cgcaaaaacg cgaatgcgcg aaagcggctg acgaatcttc atcccggatg
     2941 ctgcgcgtgc agcacggcca agggacgctg cctgcaaaac caggtctcct tcctctagaa
     3001 tttttgcgtc aaagaaagat gcgtcatact caggccagcc agccagattc gcactgatgg
     3061 gcgcgctttt ggactgcgat gccacgagac cacgatagag ttcctctcca aggaaaggag
     3121 caaaaggcat gatcaaacga gcaatcgtca cgaggcagcg atacaaagta tcaaatcctg
     3181 tgagcttctc cgcatctctt tctcccttcc agaaccttct gcgattgcgg cgaatgtacc
     3241 aattggaaag atcatcggta aagagttcta caagccgacc cgctttttga gcatcgtatt
     3301 cgttcatcga ggcggtgacg tcacgaatga gcacctgaag tctcgacaga atccatctat
     3361 ctatgtccgg acgttctttg aaaggcagag cgcccgcaac cggattcttc tgccattcat
     3421 tgatttcgat tccatccaca ttcgcataga gggaaaagaa accggcgctg ttttgcagca
     3481 tgttgaaaaa tccgaataca tctccaattg gatcctgatc cgttcctggt tttcccatct
     3541 tgcgcgaatt gccgggagga gcgcccacca ggaaatgcca gcgaatcgca tctgcgccgt
     3601 gcatttcgaa gacctgccac ggatccacag tgttgccctt gcttttggac atcttttcgc
     3661 ctttgccatc caggacatga cccagacaga ctacattctt ataggatgtt tcatcggaaa
     3721 ccatcgtaga gatggcaagt agagtgtaga accagccgcg agtctgatcg atcgcttcgc
     3781 agatgaagtc agctggaaaa tatttcgcca gttccttttt gcctgcaggc gaagttcccc
     3841 actgcgcgta aggcatggct ccagaatcaa accagcaatc aatcacctct ggaactcttt
     3901 tccattcctt tgcttcttcg cctgaagtgg cggctttcga actcttgcct gatttctttt
     3961 gcgactttgc cgcagttcca gctgcctttg ccgtttttgc tgcaccttcc ttccacgtta
     4021 tgcgatcaat gaaaggtctg tgcaaatcca gttcagtcaa tttctgaccg gtgagcttct
     4081 ccagctctgc aatgctaccg atgattttaa aattctcccc gtccgtccag accggaagag
     4141 gtgttcccca gtagcgttcg cgcgaaaggg cccagtctct attgttttca agccagtttc
     4201 caaatcgacc ctgcttgaca tgatcgggga cccagttgat agaatcgttg ttttttacca
     4261 gatgttcacg cacgcgcgta gtgcgaatgt accaggcgtc ctttgcaaag tacagaagtg
     4321 gggcgcctgt tctccagcca aaaggatatt cgtgtttgta tctttcagac ttccagacaa
     4381 ggccgcgttc tttgagatcg cgtacaaggt ccttgtcagc atccttgaaa aaaactccag
     4441 cctgaggtgt gccggaaaca aacttaccat ccaggcccac cgcatacaac accggaagat
     4501 catgagtctg gccgacgacc aggtcatctg caccataggc aggagcaatg tgaacaatgc
     4561 cggtacctgt atccatggtt acgaaatccg cggtgtagac gcgaaatgct ttgtcccctt
     4621 cggttggcag ccacgggaaa agacgttcgt agcgaacgcc cgcaagatcg gatcctttca
     4681 tttttttaat gatggaatga gaggtaacct ttgtattaga gagaacagat tcgagcaata
     4741 cttcagcaca gatgattttt tcggaaccgt cttcgcgtac tgcttctacc agcgcgtaag
     4801 attcattcgg agcgatcgca agtgccacgt tgcttggaag agtccaggga gttgtggtcc
     4861 agacgagaaa agaagtatc
//
LOCUS       DAORFF010000275         4873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9840_length_4873_cov_3.301370, whole genome shotgun sequence.
ACCESSION   DAORFF010000275 DAORFF010000000
VERSION     DAORFF010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9840_length_4873_cov_3.301370"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PKK76_11520"
     CDS             complement(<1..661)
                     /locus_tag="PKK76_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002743660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNL69149.1"
                     /translation="MNFDIPDNVLSLQKKIRLFVEELCIPAEDKYDYETGRMPDSVVE
                     PLRREAKARGLWTPHLPESEGGLGLDMVGTALVFSELGRSFIAPYVCNCDAPDEGNMH
                     LLHLAANEEQKNLIYHPLRKGEIRTGFAMTEPAPGAGADPQSLQTNAEKKGDKYVING
                     RKWYCTGANGAKYLIVMAKVGGSFRRTTMFLVPTNAKGYTMVREIGVLGSHGPGGHCE
                     LL"
     gene            complement(697..1101)
                     /locus_tag="PKK76_11525"
     CDS             complement(697..1101)
                     /locus_tag="PKK76_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69150.1"
                     /translation="MISMDSSTRAEMIRKGNQAFNEGKYPEARELFTKADYKDGLVRL
                     GDYYMFDRRLPLLAYGYYKRAGAMRKIEEIQKRMVLALGEWIGRDKIKPESLQRLESG
                     FAPDMTTDREGMVSVPVSPFLKDAALKILAKK"
     gene            complement(1151..2254)
                     /locus_tag="PKK76_11530"
     CDS             complement(1151..2254)
                     /locus_tag="PKK76_11530"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL69151.1"
                     /translation="MIRFSTIPILVLLWTSSECVHSPRCEEDIGCSVTTALLYTRTLA
                     PKFLYTTNGSASGTISMYSVDERTGVILPLSPASVASGNLGTFLTMDPSRNFAFVDDV
                     GTNVYTYAMNQLTGNLTQTAVTANGPNSNPFVVENNSRFAYGTSNGSTTINIYSYNAS
                     TGAVSLINTATNGAATATQSTIMDPQGRYLYTSNQTSNDVSQFAINTSTGALTSLGTA
                     VSATQPGGICNDSQSRWLYATNTGGASVAGFSIQSNGSLVSISGSPFATPATGTSCAV
                     TPDGAFLVIASTGLTVFPINQSTGALGTQTTYTAAANGVAISPSGQFVFSTDATTTRS
                     FRMNKTTGALTLINTQPAGTGPSLSVLVNLYVF"
     gene            complement(2263..2598)
                     /locus_tag="PKK76_11535"
     CDS             complement(2263..2598)
                     /locus_tag="PKK76_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNL69152.1"
                     /translation="MAKPEEFIAVNSLIGEGSEFRGEFKVKEAIRIDGYFKGSIITEG
                     KVVVGKNGVVETDLRAGIVVVGGEVRGSIHAARRVTLLSTCRLRGDIVTKSLLVEDGV
                     KFEGRCTVA"
     gene            complement(2574..3698)
                     /locus_tag="PKK76_11540"
     CDS             complement(2574..3698)
                     /locus_tag="PKK76_11540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNL69153.1"
                     /translation="MARTPEKKKKPAKKAAAKQVDDFMPEPQPPRPTRWQQFRTRFEE
                     IRERIHKKGRERITIMVIPHTEKQIFNLHVNLYAVVTAVAVVTVVLGVSILTLAGKSG
                     EDIQNYDMGLTNSQFNVQTTRMAEEMVPLHNLIQTYSNTIAELYFKLDGDAPGEAQGG
                     AASAVLGQEIESLRSLVEECKKLGEDCDQGRTEEILRRVIFLSRQDNQNLRRAVELSD
                     KILAQLKTKEKRNLLKNTPSIWPVKGYVLSAYGWQVDSLRGRKVFRPGMEIGALPGSE
                     VVATAPGIVERVDYTPELGLHMWVSHRFGMQTLYAHLDRVVVPKDEKVQKGQLIAYSG
                     KTGMTSVPMLYYEVHVGTVAYNPYAFLNHIQDEWLNPKNL"
     gene            3783..>4873
                     /locus_tag="PKK76_11545"
     CDS             3783..>4873
                     /locus_tag="PKK76_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNL69154.1"
                     /translation="MTESAKEPAATHAPWQREFFKNIEGFMQVGIPEDKAKEILMTFL
                     KLSSSTPMPRVMESFRSPEILEEVGVHTKQNPELRDFMATFLDPVMRNFTVEGLENLS
                     GILPLLGKYPMTLISNHLSHLDAPAIYNLLYRQGGDARKLADSLVFIAGRLAFEPDFT
                     RLGLYMFDTLLVCSKLDMTENPGLADLMTRINMRAFRHANQLQKEGKIIAIFPEGTRS
                     RTGKLVNFVDTVYHYVANKIIVPVSLAGTEEILPTQSFLFNASKGKLVLGKPVLVGDL
                     PKSQMASLPEYVQRLAIPEVGDKKQFVIDNLALLIGQNLHKHRHGTYRNLYRGDDLGS
                     NVLITRPKTPTERVVVLGHSPYGTAAATIL"
BASE COUNT         1114 a         1266 c         1230 g         1263 t
ORIGIN      
        1 agagaagttc gcagtggccg ccagggccgt gagatccaag cactccaatt tcgcgcacca
       61 tcgtatagcc tttcgcattt gtgggaacga ggaacatggt agttctgcgg aagcttcctc
      121 cgacctttgc catgacgatc aggtatttcg cgccgttggc tcctgtgcag taccacttgc
      181 ggccattgat gacgtactta tctccctttt tctctgcgtt tgtctgcaga ctctgaggat
      241 ccgcacctgc accaggggcc ggttctgtca tggcaaaccc tgttctgatc tctcctttgc
      301 gaagaggatg atagattaag ttcttttgct cttcgtttgc cgccagatga agaaggtgca
      361 tgtttccttc atccggtgcg tcgcaattgc agacgtatgg agcaatgaaa gatcttccga
      421 gctctgagaa tacgagagct gtgcccacca tgtccagccc cagtccgcct tcactctcgg
      481 ggagatgcgg ggtccacaga ccgcgagctt tcgcctcgcg gcgaagcggc tctactactg
      541 aatccggcat tcgcccagtt tcgtaatcgt atttgtcttc tgcagggatg cagagttctt
      601 ctacaaaaag acggatcttc ttttggagcg aaaggacatt gtccgggatg tcgaaattca
      661 tcgactcagc ctggaagggt ctttctaacg cgcgacttat tttttggcca ggatcttaag
      721 tgcggcgtct ttcagaaagg ggctcaccgg cacgctaacc attccttctc tgtctgtggt
      781 catgtctgga gcaaagcctg actcaagtct ttgcagggat tctggcttga tcttatctct
      841 cccgatccat tctccgagag cgagaaccat tctcttctgg atctcttcaa tttttcgcat
      901 agccccggcg cgtttgtagt aaccataggc tagaagaggc agacgacgat cgaacatgta
      961 gtaatcgccc aggcgaacga ggccgtcttt gtaatctgct ttcgtaaaca gctcgcgggc
     1021 ttctggatac tttccttcat tgaaggcctg attgcccttt cggatcattt cagctcgagt
     1081 gctcgaatcc attgagatca tacaatatct ctcggcaggg tttgtcaatc ctggtagaac
     1141 gaaagaactt ctaaaacacg tacaaattga cgagcacgct cagagagggg cccgttcctg
     1201 ccggctgagt attgatgaga gtgagcgcgc ccgtggtttt gttcatgcga aacgatctcg
     1261 tggtggtcgc atctgtggag aatacaaact gccccgatgg agagatcgcc acaccgttgg
     1321 ccgctgcagt atacgtagtc tgcgttccaa gagccccggt actctgattg atgggaaaca
     1381 cagtcaggcc ggtggatgcg atgaccagga aagctccgtc tggagtcaca gcacagctgg
     1441 tgcctgtggc cggagtcgca aacggcgaac cgctgatgga tacaagagat ccattggatt
     1501 gaatggagaa tcctgccaca gaagcgccgc ccgtgtttgt agcgtatagc cagcggctct
     1561 gcgaatcatt gcaaatgcca ccgggttgtg ttgcggagac agcagttcca agagaagtca
     1621 gagcaccggt agaagtattg atcgcaaact gcgaaacgtc attgcttgtc tgattgcttg
     1681 tatagaggta gcggccctga ggatccatga tcgtggattg ggttgcggtc gcagcaccat
     1741 tggttgcagt attgatcaaa gagacagcac cagtagacgc attgtaagaa tagatattga
     1801 ttgtggtcga tccgttgctt gtgccgtacg cgaatcggct attgttctcc accacaaacg
     1861 gattcgaatt tggtccgttg gccgtaactg cagtctgagt gagatttcct gtgagctgat
     1921 tcatcgcata cgtgtataca ttggttccca catcatctac aaatgcaaag ttgcgcgaag
     1981 gatccatcgt gaggaaggta ccaaggtttc cagacgccac agaggcagga ctgagaggca
     2041 ggatgactcc tgtcctttca tccacgctgt acatcgaaat ggttcctgat gcactcccat
     2101 tggtggtgta tagaaatttg ggagctaaag tgcgtgtgta aagtagcgcg gtggtaacgg
     2161 agcaaccaat gtcttcctca cagcgaggcg aatgaacgca ctctgaactt gtccagagta
     2221 agaccagaat tggaattgtg ctgaatcgga tcaagaaacc gcctaggcaa cggtgcaacg
     2281 accttcgaac ttaactccgt cttccacaag aagacttttt gtaactatgt ctcctctcag
     2341 tctgcaagtg ctcaagagtg taactcgtcg tgcagcgtga atggagccgc gcacttctcc
     2401 gcccacgact acaatgcccg cgcgcagatc tgtttccact acgccatttt ttccaacgac
     2461 cactttgcct tccgtaatga tgctaccttt aaagtagcca tcgattcgga ttgcctcttt
     2521 gactttgaat tctccgcgaa actcggagcc ttcgccaatg aggctgttga ctgctataaa
     2581 ttcttcgggt ttagccattc gtcctggata tggttcaaga atgcatacgg attgtatgca
     2641 accgtaccta catgtacctc ataatagagc atggggacag acgtcattcc agttttgcct
     2701 gagtaagcga tcagctgtcc cttctgcact ttctcgtctt tgggaaccac aacccgatct
     2761 agatgcgcat aaagggtttg catcccgaaa cggtgggaaa cccacatgtg aagtccgagc
     2821 tccggagtgt agtccactct ctccacgatt ccaggagcgg tcgctaccac ttcacttcca
     2881 ggcagcgcac cgatttccat tccaggcctg aacacttttc tgccgcgaag tgaatccact
     2941 tgccacccgt acgcggacaa aacatatcct ttgactggcc agatgctcgg ggtgttcttt
     3001 agaagatttc ttttttcttt tgtcttgagt tgtgcgagga tcttgtcgct gagttccact
     3061 gctcttctta gattttgatt gtcctggcga gataaaaaga tgactcttct gagtatttct
     3121 tctgttcttc cctgatcgca gtcttctccg agcttcttgc attcctctac cagagacctg
     3181 aggctttcta tttcctgccc gagcaccgca ctcgccgcgc caccctgtgc ttctccaggt
     3241 gcgtctccat ccagcttgaa gtacagttct gcaatcgtgt tggaataagt ctgaattaag
     3301 ttgtgaagag gaaccatttc ctctgccatg cgtgtggttt gcacattgaa ctgcgagttg
     3361 gtcagtccca tgtcatagtt ttgaatgtct tcgccgctct ttcctgcgag agtcagaata
     3421 gagactccca gcacaacggt cacaacagcc acagccgtta cgaccgcgta caggttcacg
     3481 tgcaaattga agatctgctt ttcagtatgc ggaatcacca tgatggtgat tcgttcgcga
     3541 cccttcttgt ggatgcgctc tcgtatctct tcgaagcgag tgcgaaactg ctgccatctg
     3601 gtaggacgcg gcggctgcgg ttcgggcatg aagtcatcga cttgctttgc ggctgccttc
     3661 ttcgcgggct tctttttctt ttccggggtc ctggccatga ctcttcttta tagcttcggc
     3721 acgcagtgcc ctgtcaatga aatttgcttg cacgcggggt actgccaggt cagacacctt
     3781 ccatgactga gagcgcaaaa gagccggccg ccacccatgc cccgtggcag agggaatttt
     3841 tcaagaacat agagggattc atgcaggtgg ggattcctga ggacaaagca aaggaaatcc
     3901 tgatgacctt cctgaagctc tcatcaagca cacccatgcc cagagttatg gagagctttc
     3961 gcagtccgga gatcctggag gaagtgggtg ttcacacgaa gcagaatcca gaactgcgcg
     4021 atttcatggc cacgttcctg gatccagtca tgcgcaactt tacagtggaa ggtcttgaga
     4081 atctatccgg catcctgccc cttctcggca agtatccaat gaccctcatt tccaatcacc
     4141 tcagtcacct ggacgcgcct gctatttaca atttgcttta tcgccaggga ggagatgcgc
     4201 gcaagctcgc agacagcctg gttttcatcg ccggcagact tgcttttgaa ccggacttca
     4261 ctcgccttgg tctgtatatg ttcgatactc ttctggtttg ttccaaactg gacatgactg
     4321 agaatccagg cctcgcagac ttgatgactc gcatcaacat gcgcgcgttt cgtcatgcaa
     4381 accagcttca gaaagaggga aagatcattg cgatcttccc ggaagggact cgcagtagaa
     4441 caggaaagct ggtcaatttt gtagatacag tttatcacta cgtcgcaaac aaaatcatcg
     4501 tgcctgtgtc tctcgcaggg acggaggaaa ttcttccaac ccagtctttt cttttcaatg
     4561 caagcaaagg gaagctggtg ctcggcaaac cagtgttagt cggagacctt cccaaatcgc
     4621 aaatggcttc cctgccggaa tacgtacaga gactggctat tcccgaagtc ggagacaaaa
     4681 agcagtttgt gattgataac ctggcgctgc tcatcggaca gaacctgcac aaacacaggc
     4741 atggcacata caggaatctc tatcgcggag atgatctggg tagcaatgtt ctcatcacaa
     4801 gaccaaaaac tccgactgag agagtggtcg tgctgggaca ttcgccgtac ggaacagctg
     4861 cggccacgat tct
//
LOCUS       DAORFF010000276         4857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9903_length_4857_cov_3.289879, whole genome shotgun sequence.
ACCESSION   DAORFF010000276 DAORFF010000000
VERSION     DAORFF010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9903_length_4857_cov_3.289879"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PKK76_11550"
     CDS             complement(<1..591)
                     /locus_tag="PKK76_11550"
                     /inference="COORDINATES: protein motif:HMM:NF017893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HNL69155.1"
                     /translation="MIRLTIKMALIGALSTMLLIPTAYICSLTKERKDRRDEVARSTG
                     NLWGSHHEIGTILEAGKESILPQSVDVTGTVLAETRNRGIYSLPFYSADLTMQVQFFL
                     ERESQASVIRLYGVKASEADFLTSKLDDLEIRFKGDDEPRQVAAHAGTLSRGTHKLTI
                     HFKMNGTQRLHFVPAANTLTVDLKSNWPHPNFSGSIL"
     gene            744..1121
                     /locus_tag="PKK76_11555"
     CDS             744..1121
                     /locus_tag="PKK76_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL69156.1"
                     /translation="MKGKRILVIDDDESTRALVSHALTKAGAVVVSAADADGAMKAAS
                     HDRVDLILLDLKMPDFDGMTLLRTLGMDADPPPVVILSGVESKHITQQAVVDGAVGFV
                     HKPIDLRTFAAKVSRILNWAELE"
     gene            complement(1143..2519)
                     /gene="creC"
                     /locus_tag="PKK76_11560"
                     /pseudo
     CDS             complement(1143..2519)
                     /gene="creC"
                     /locus_tag="PKK76_11560"
                     /inference="COORDINATES: protein motif:HMM:NF008312.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
     gene            complement(2557..3216)
                     /gene="creB"
                     /locus_tag="PKK76_11565"
     CDS             complement(2557..3216)
                     /gene="creB"
                     /locus_tag="PKK76_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002761830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HNL69157.1"
                     /translation="MKILIVEDEPAIADALLFALRADGFTADHARTAQEARPLVADAD
                     LLILDVGLPDTNGFDLLRDLRTKSQVPVLILTARSEEVDRIVGLELGADDYVTKPFSP
                     REVVARARAILRRSSKKPDKQSAFDVDESRAVIRYFGKVLILSRTEYRMLALFLSRPG
                     WVFTRENIMDAAWEEPEESFDRSVDTHIKNIRAKLKEIRSDLDPIVTRRGIGYSIQED
                     L"
     gene            complement(3213..4769)
                     /locus_tag="PKK76_11570"
     CDS             complement(3213..4769)
                     /locus_tag="PKK76_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HNL69158.1"
                     /translation="MNAETRIERVTIYSDRARIERVARLHIPPGRHVIAIPDLPVLAD
                     ENSVRASVRGAKLLSLDVKRSRYLEPPSANAHELEKKIEDLTDQISLIDRSIAVEQER
                     SAHFEKLGGETLAIARGIASGRMEISKHTELESSIAEEKKASTNRIIQLSKQKRQTVR
                     ELAKADLEYKNAGNAVSRERIECSLEVESEGKEVEMQISYIVGSASWLPRYDFRLGSK
                     LLMQYLGAIRQKTGEAWQNVELSLSTAPASDQIAPVEFSTWFLHEYVPPPPRPAAAPG
                     MIGAPPMQASARAADAPFEQEEVDALLYAGMEDSTSVSSGASITYTLTSRFDIPGNGE
                     EKKVPVTVQEFARELEYVTAPANGEQAFRRVRFTNDSAYLLLGGPGFVYYDQDFVGNT
                     SIEETPPGQKRELYLGIESRIRVKRELKLRETDKKLLGDTKRIRFRYQIDLENHLPEE
                     AVIILREQIPASTHASIKVKLETQESKPELPLPGILEWKFTLPAGAKREVSYDYVVEH
                     PQNMTILGLH"
BASE COUNT         1039 a         1228 c         1248 g         1342 t
ORIGIN      
        1 caggatcgat cctgaaaaat taggatgcgg ccagtttgat ttgagatcta ccgtcagagt
       61 atttgctgct ggaacaaagt gcagtctctg ggttccgttc attttgaaat gaatcgtaag
      121 tttgtgagtg ccgcgcgaaa gggttcctgc gtgggccgcc acctgcctcg gttcatcatc
      181 tcctttaaag cgaatctcaa gatcgtctaa cttagatgta aggaaatctg cttcactcgc
      241 ttttaccccg tagagtcgaa tcaccgacgc ctggctttcg cgttccagaa agaattgcac
      301 ctgcatcgtg aggtccgcgc tatagaacgg cagcgagtag atgcctcgat ttctggtttc
      361 tgccagaacg gttcctgtca catccacgct ttgagggagg atgctctctt ttccggcctc
      421 cagaatggtc ccgatttcat gatggctgcc ccagaggttt ccagtgctcc tggccacttc
      481 gtctcttctg tcctttctct cttttgtaag agaacaaatg tatgctgtgg gaatgagcaa
      541 catcgtggac agagccccga tcagcgccat tttgattgta agtcgaatca tatctatctc
      601 ctttgataca gacagaatcg cacgctcgag tggacgcagt atgagcgaag tgtgtggatt
      661 gtgtgagccg gaagattttt tgagactgct gtggcctcac ttgacagggc ggaccagtcc
      721 gatacttacc gggtgaggcc gttttgaaag ggaagagaat cctggtcatt gacgacgacg
      781 aaagcacgcg cgccctggta tcgcatgctc tgacgaaagc gggggcagtc gtagtttctg
      841 ccgcggacgc ggacggtgcc atgaaggccg ccagtcacga tcgcgtggat ctgatcctcc
      901 tggatctgaa gatgcccgat tttgacggga tgacgcttct tcgcactctg gggatggatg
      961 cagacccgcc tcccgtagtc attttatccg gagtggaaag taagcacatc acccagcagg
     1021 cagtggtgga tggagcggtc ggatttgttc acaaacccat tgatctgcga acattcgccg
     1081 cgaaagtaag caggattctg aactgggcag agctggagtg attttcacca ggttttagca
     1141 tatcatagcg gaatagaaat agaagcgcac gcgccactcc ctgtgttctg gatctctgct
     1201 tttcctccgt gcagttccgc cgcctccttt acaaagcaca aaccaagtcc tgtgctcttt
     1261 cgtttgttgt caggacgtgg cagggaataa aatctttcaa acgctcgcga gatagcgaat
     1321 tcaggaatgc ccgggccttg atctgttacg gaaaatttta gaaagccttc ttccattttc
     1381 gtttccagtg caatggtaga atggtccggt ccaaagtcac ttgcgttttc tactagattg
     1441 accagggccg attccagtac agcgcgcgat cctgcaatcg tcacatcttc tgatttaact
     1501 tccagccgct gcgaacgctt ctcgagcctg gatgcagtgc gaaggcgaat ttcctggatg
     1561 agttcgtttg agccaatccg ttcgcgcgat gcgagagact tctgaccttc tatgcttgcg
     1621 agttcgagca gtcttctggt gaggtcttct atgcgagacg attcggtgag tatgttttca
     1681 agaaattttt tcctggattc ctcatccggg tttccttctg ctagaatttc tgcggcggcg
     1741 cgaatggatg tgacagggct cttgatttcg tgggtcattg tttttacgta ctgctccacg
     1801 taagattttc cttccagttc ctgtctcagg ttttcaaatt cacgcgccag ctctccgatt
     1861 tcgcctgctc caacatccgg tggtgagagc ctgccgtgct ttcgcacaga aactatgtac
     1921 tgtgtgagga ttcgaagcgg cctcgtgacc cagaagaaag tggccagagt caagagagtc
     1981 atggcgatca ctgcaaagcc ggccccctgg agtaattttc tctccgcgat tcgcacaaat
     2041 ggctgaatgg aatcgtttgg tttgaccact gtcaccacgc ctacaatttg cccatcgtgc
     2101 cgaacgggcg cggcaacgta gagagatccg gtcatcggat catccttctt gatgcgcgtg
     2161 gcgcgcactc cgtattctcc tctcaatgtt cgcctgacgt cattccagct tgagaaatcc
     2221 tgacctgtgg attttccttc ggagtcgtag attacaattc cgcgaccgtc tgttacatat
     2281 acgctcacgt tgattttgtc tttcttcaaa gcatagatct ttgccttgaa agccatcgat
     2341 cctgcccgcg aaactacttc tttgagaaga gcagggtccg gtgcggcgga agcgtccctt
     2401 tccaggagag ctgccagaag atgcgctgtg tcgttgagtg attcttccag ggcttccagg
     2461 tagcggggtc tgatttcatc ctggatcagg cgatacagta gaaagaatcc gcctgcgaag
     2521 gtaaaaaaaa actgaggaag attttagcgg tgagtttcac agatcctcct ggatcgaata
     2581 accaatgcct cttctcgtaa caatcggatc caggtccgaa cggatttctt tgagtttcgc
     2641 gcggatattt ttgatgtggg tgtccacgga tcgatcaaag ctttcttctg gctcttccca
     2701 ggccgcgtcc atgatattct ctcgcgtgaa tacccagcca ggtctggaaa gaaaaagagc
     2761 gagcattctg tattccgtgc gtgatagaat gagcaccttg ccgaagtagc gaatgactgc
     2821 tctgctttcg tccacgtcaa aagcgctttg tttgtccggc ttctttgaac tgcgtctcaa
     2881 gatggcgcgt gctctggcca ccacttcgcg cggggaaaat ggtttggtca cataatcgtc
     2941 tgcacccagc tccaggccta caatgcgatc cacttcttcc gatcgcgcgg tgaggatcaa
     3001 aacgggtacc tgggatttcg tgcgaagatc gcgcaataga tcaaagccgt ttgtatcagg
     3061 aaggcctaca tccaggatga gcaagtctgc gtctgcgacc agaggtcgcg cttcctgtgc
     3121 tgtccttgca tgatctgcag tgaagccgtc cgctcgaaga gcaaacaaca gagcatccgc
     3181 aatggcgggt tcatcttcca cgatcagaat tttcaatgca gccccagaat ggtcatgttt
     3241 tgaggatgtt ccaccacgta gtcgtaggat acttcgcgct ttgctccagc gggcaaagtg
     3301 aacttccatt ccagaatgcc aggtagagga agttctggtt ttgattcctg ggtttccagt
     3361 ttgaccttga tgctggcgtg cgtggacgct ggaatctgtt cgcgcaggat gatgactgct
     3421 tcctcgggca gatgattttc taaatcgatc tggtagcgga agcgaattct ttttgtgtct
     3481 cccaggagtt tcttgtctgt ttcgcgaagc ttgagctcgc gtttgactcg aatgcgactc
     3541 tcaattccca ggtatagttc tctcttttgt cccggaggcg tttcctctat ggaagtattt
     3601 cccacaaaat cctggtcgta gtacacgaat cctggtccac ctaacagcaa gtaggcggaa
     3661 tcatttgtga aacgcactct tctgaatgct tgctctccat ttgccggtgc tgtcacgtat
     3721 tctagttcgc gcgcaaactc ctgaaccgta acgggcactt tcttttcttc cccgtttccc
     3781 ggaatgtcaa atctggacgt gagagtgtat gtgatgcttg cgccagagga cacagaagta
     3841 gagtcctcca tgcccgcgta gaggagggcg tctacctcct cctgctcgaa tggagcatct
     3901 gctgcgcgcg cgcttgcctg catgggtgga gctccaatca ttccaggtgc ggcggcggga
     3961 cgaggaggcg gagggacgta ttcgtgtaaa aaccaggtgg agaattccac aggagcaatt
     4021 tggtctgagg cgggagccgt ggacaaagag agctccacat tctgccaggc ttcaccggtt
     4081 ttctggcgaa tggcacccag gtattgcatg agtagtttcg atccaagtct gaaatcatac
     4141 ctcggaagcc aggacgcaga tcccactatg taagagattt gcatttctac ttctttgcct
     4201 tctgattcta cttccaggga gcattcaatt ctttctctcg aaaccgcatt ccccgcgttc
     4261 ttgtattcta aatctgcttt tgccagttct cgcactgttt gtcttttttg tttagaaagt
     4321 tgaatgattc tgtttgtgct tgcttttttc tcttctgcaa tagaagattc aagctccgta
     4381 tgttttgaaa tttccattct tccggaagcg attccgcgcg cgatggccag cgtttcccct
     4441 ccgagctttt caaaatgcgc ggatctttcc tgttccacag cgatgcttcg atcgatcaaa
     4501 gatatttgat ctgttagatc ttctattttc ttttcaagtt catgcgcatt tgcagaagga
     4561 ggttccagat agcggctgcg cttaacatcc agggaaagaa gctttgctcc tcgcacagag
     4621 gctcgaacgc tattttcatc cgctagaacg ggcaaatccg ggatggcgat gacgtgtctg
     4681 cccggtggta tgtgaagcct ggcgactctc tcaatgcgcg ctcgatcgct atagatggtg
     4741 acgcgttcta ttctggtttc tgcgttcatt cctttgcttg agaaatgaac ccccgatcca
     4801 ttcggtcaag ctatatggaa tccagttccg cgatttcttc tgcatacccc gcggata
//
LOCUS       DAORFF010000277         4851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9923_length_4851_cov_3.996872, whole genome shotgun sequence.
ACCESSION   DAORFF010000277 DAORFF010000000
VERSION     DAORFF010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9923_length_4851_cov_3.996872"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PKK76_11575"
     CDS             <1..353
                     /locus_tag="PKK76_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNL69159.1"
                     /translation="LAEDLIRFAGLVPHQDIQIHYIGLRPGEKLYEELLLAEEGIEPT
                     RHEKIHIANSRISHLPTLLEKVHELEEAARRGSRPDMDAIMSSIVPEYRPVSPMIRPA
                     DRAQEKENARSRKI"
     gene            complement(379..1470)
                     /locus_tag="PKK76_11580"
     CDS             complement(379..1470)
                     /locus_tag="PKK76_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69160.1"
                     /translation="MSRSSLVFLILASAALHVAALAIPAFKAGHLSAAVRFGCLSPTI
                     CYAMKNKAILPENAVVYKSGGYDGQFYFYSAMRVFRGVQVEFDSAPFRLARIGYPVLA
                     GMGALAGPGYLLYSMYFIPLALHLFVVFFLRSDPLRASIFAFNPISVLSAGLFLADGM
                     ALSLLVLALELAFKEKHSLVVLIIAALSMLCKETAAAGALSALFLFLRFRKPVFVALV
                     VACLPMIVWWFATGFSPFFAATRGTAGGPGVWNYVQHPDALLSGRSLLFLFYILGCIV
                     LFRLDLKQERFLAGAALIACGFLLSLTASSEYWDNFANVARLFLPVCAGLLLAARQWK
                     GRIVSGTFLFFSLLLLFREAKSVYDIEVW"
     gene            complement(1467..1847)
                     /locus_tag="PKK76_11585"
     CDS             complement(1467..1847)
                     /locus_tag="PKK76_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69161.1"
                     /translation="MANVRKPACPLDPASVKELFAVEVAAVDCSQWKPVFFEGVREIL
                     KSVEKQKSQTILYSVPPDLALQLSLCGVSVTEDSAVIQPDCLSRGIGQCPFCGAAIRF
                     EKAGRHQCPSCQNLLMVEDGAISA"
     gene            1902..2714
                     /locus_tag="PKK76_11590"
     CDS             1902..2714
                     /locus_tag="PKK76_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69162.1"
                     /translation="MAGVNKARLIWTWTQEAVWLALLVLQLPFVFVQKWSSHGRRKEP
                     VLLLTDLGLSVFAYFRIRMALARAGFPVIVLSIGSVSSLSSQARQLARTLEQLNIQKG
                     ILVAHGRGGLTALALPDSGRRRIQHLVTLGTPFHGSRFFTPFAFVPAFGDMNVGSDFL
                     LLHRMNALLFPGFSPFSAYKDEFIYPGNLGHFGQGRDLILDRNGHMNLILDGENIETI
                     VQHIESLHPEPAANPEVRAAKPPEVAPPAAKKSAKKSSKKKPSRPGRKKGKR"
     gene            2711..3013
                     /locus_tag="PKK76_11595"
     CDS             2711..3013
                     /locus_tag="PKK76_11595"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNL69163.1"
                     /translation="MNLKEIQRVIDDWIGKNGGYYSELTNLARLTEELGELARLYARR
                     EIPARDLEDVSRHKMQEEMGDIVFALCVLANQAGIDLETSVQDVLNKVKLRDADRH"
     gene            3051..3449
                     /locus_tag="PKK76_11600"
     CDS             3051..3449
                     /locus_tag="PKK76_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69164.1"
                     /translation="MRAILASIACLLLVQCAGANAGIAVSNVPTEGQTYTVLGTAETT
                     KSWWSLDLAVLAFPLGEPPVAEAVEDLLKQKEGNALINLRFWTDRTTILFMTRHRFHL
                     QADVIRFDPKVDPDKDKKDKAKDDKDKLKK"
     gene            3446..4030
                     /locus_tag="PKK76_11605"
     CDS             3446..4030
                     /locus_tag="PKK76_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69165.1"
                     /translation="MKRFLFIAILAAGACTPGGLIDKGGYRDQGGIQPQASRTHGVAY
                     KILCYDSQEARRREGLPEGAEKPPGPPVRIEDPATYKGGCEEEGESSVFFLFNIVPVT
                     PPLNPEYAIGTAVQKLEGDTMIGMKTWQETHYYSILGRVSVFKVKGLVIKFDNKEPTD
                     SKSDSKDKDKKGDKATDKKDDKTDKKDLKADQKK"
     gene            4027..4314
                     /locus_tag="PKK76_11610"
     CDS             4027..4314
                     /locus_tag="PKK76_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69166.1"
                     /translation="MKVIAFAVLLLLSGCIVENDTVDTADAYSEIYASIQFKARQCGN
                     QPAYPLLVPENPPSYGTRLCSLSIIRQDCPFTNYPLFCVEMFGIDLPGIGP"
     gene            4311..>4851
                     /locus_tag="PKK76_11615"
     CDS             4311..>4851
                     /locus_tag="PKK76_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69167.1"
                     /translation="MMKWRALLVFFLLFPATVFAIDVKIKIEDKKAGKPVSDAQVIVM
                     ETKEKFFSDETGEAIIRIPAKGFYTIRVISPSGEIVQPRVEVISPNQTVTIFLGIEEE
                     KPIPTNTPQKVTAEEGISVTGKRERQKVSRYQVRIDEVKRIPGQFGEALRGLETLPGV
                     NAPPFGNGDIAVRGANTNSN"
BASE COUNT         1384 a         1234 c         1217 g         1016 t
ORIGIN      
        1 acctggcaga agacttgatt cgattcgcgg ggcttgtgcc gcaccaggac attcagattc
       61 attacattgg tttgcggcca ggtgaaaaac tatacgaaga gcttcttctc gccgaagaag
      121 gaattgaacc aacgcggcac gagaaaatcc atatcgcaaa ctccaggatt tcgcaccttc
      181 ctactctcct tgaaaaagta catgaactcg aagaagcagc cagacgcgga tcgcgtccgg
      241 atatggacgc gatcatgtca tccattgttc cggaatacag acccgtaagt ccgatgattc
      301 gcccggcgga cagagcgcag gaaaaagaaa acgctcgatc gcgaaagatc tagcaatcca
      361 ctcatctcag actggagttt accagacttc aatatcgtag acggactttg cttcgcgaaa
      421 taggaggagc aaagaaaaga acaagaacgt tcccgaaacg atgcgtccct tccactgtcg
      481 cgccgccagt aaaagtccag cacatacggg caagaacagg cgcgccacat ttgcgaagtt
      541 atcccagtac tctgaagatg cagtcagaga aagtaaaaaa ccacaagcaa tcaaagccgc
      601 gcctgccagg aatcgctcct gctttagatc caggcgaaag aggacaatgc atccaaggat
      661 gtagaataaa aacagcaaac tgcggccgga aaggagtgca tccggatgct gcacgtaatt
      721 ccatacaccg ggcccgcccg cagtccctct ggtagcagca aagaaagggg aaaaaccagt
      781 cgcaaaccac cagacaatca taggcaaaca ggcaacgacc agagccacaa acacgggttt
      841 gcgaaatcga aggaatagaa ataaagcgga tagagcgccg gccgccgcgg tttctttgca
      901 gagcatgctc agcgcggcta tgatcaaaac gacgagcgaa tgtttttcct tgaatgcaag
      961 ctccagagca agaaccaata gcgaaagcgc catgccgtct gccagaaaca accccgctga
     1021 caaaacagag atcggattga acgcgaagat actggcgcga agcggatcgg atctaagaaa
     1081 gaatacaaca aacaaatgaa gtgcaagggg aataaaatac atggagtaaa gaaggtagcc
     1141 tggaccagcc agcgccccca ttcctgcgag cactggataa cctatgcgag ccaggcgaaa
     1201 aggagcagaa tcaaattcaa cctgaactcc gcgaaaaacg cgcatcgcag aatagaaata
     1261 aaactgcccg tcatagccgc cgctcttata gactacggca ttctcaggaa gaatcgcctt
     1321 gttcttcatc gcataacaga tagtaggtga aaggcagcca aatcgaacgg cagcagacaa
     1381 atgccccgcc ttgaaagcgg gaatggcgag agctgccaca tgaagcgcgg cggacgccag
     1441 aatcagaaat accaggctcg atctgctcat gcactgatgg cgccgtcttc caccatcagc
     1501 aggttctgac aggacggaca ctgatgtctg cctgcttttt caaaacgaat tgccgcaccg
     1561 cagaaaggac actggccgat cccacgcgaa aggcaatcag gctgaatgac cgcagagtcc
     1621 tccgttacag aaacaccgca cagactgagt tgaagcgcca gatcaggtgg aacactgtag
     1681 aggatcgtct gggacttttg cttttctaca gacttgagaa tttcccgaac gccttcgaaa
     1741 aacacgggct tccattgact gcaatccact gccgcaactt ccacagcaaa cagttctttg
     1801 actgaggctg gatcaagagg gcatgctggt ttgcggacat tcgccataga tcatttgcag
     1861 aatgatttgc gctcctggca accatcttcc actctggatt catggcagga gtgaacaaag
     1921 ctcgcctgat ctggacatgg acccaggagg cagtgtggct ggcgttgcta gtgcttcagc
     1981 tcccgtttgt ctttgtccag aaatggtctt cgcatggaag acgcaaagag cccgttctct
     2041 tgctcacgga tctgggtttg agtgttttcg cttactttcg cattcgaatg gctctggccc
     2101 gggccggctt cccggtgatt gttctttcga tcggaagtgt ttcttctctc tcctctcagg
     2161 ccaggcagct ggccagaaca cttgaacagc tcaacattca aaaaggaatc ctggtggctc
     2221 acggaagggg cggtctgact gcgctggctc ttccggattc tggaagaaga cgtatccagc
     2281 acctcgtgac tctgggtact ccgtttcacg gttcgaggtt cttcactccc ttcgcttttg
     2341 ttccggcctt cggagacatg aatgttggaa gtgacttctt gttgcttcat cgcatgaacg
     2401 cgctcctgtt tcccggattc tctcctttca gcgcctacaa agatgaattc atttatccag
     2461 gaaacctcgg gcactttggt cagggacgag atctgatact cgatcgcaat ggtcatatga
     2521 atctgattct cgacggagag aacatagaga cgattgtaca gcacatcgaa tctctccatc
     2581 cagagcctgc ggcaaatccg gaagtccgtg ccgccaaacc gcccgaagtc gcacctccgg
     2641 cggcaaaaaa gtccgcgaaa aagtcgtcaa agaaaaagcc atccaggcca ggccgtaaga
     2701 aaggaaagcg ttgaatctaa aggagattca acgagtcatc gacgactgga ttgggaaaaa
     2761 cggcgggtat tattctgagc tcaccaatct tgctcgtttg acggaagaac tgggagaact
     2821 ggctcgcctc tatgccaggc gcgaaattcc cgcgcgagac ctggaggatg tttcgaggca
     2881 caaaatgcag gaagagatgg gcgacatagt atttgccctg tgcgtacttg ccaatcaggc
     2941 cggaattgat cttgagactt ctgtgcaaga tgtgctgaat aaagtcaagt tgcgcgacgc
     3001 agaccggcat tagaagttgc cactcccctg tcgcctttga aatctgccac atgcgcgcaa
     3061 tccttgcttc catcgcatgt ctactgctgg ttcagtgtgc cggcgcaaac gcaggcatcg
     3121 cggtgtccaa tgttcctaca gaaggccaga cttacacggt tctgggcact gccgaaacca
     3181 ccaaaagctg gtggagtctg gatctggctg tgctggcgtt tcccctgggt gaacctccgg
     3241 ttgcggaagc tgtagaagat ttactcaagc agaaagaggg aaatgccctc atcaatcttc
     3301 gattctggac agacagaacc accatcctgt tcatgacccg tcatagattt catcttcagg
     3361 cagacgttat tcgctttgat ccaaaggttg atcccgacaa ggacaagaag gacaaggcaa
     3421 aggacgacaa agacaaacta aagaaatgaa acgatttctt ttcattgcaa ttttagctgc
     3481 aggagcatgc accccgggcg gcctcattga caaaggaggc tacagggatc agggcggaat
     3541 tcaaccccag gccagtcgca cgcacggtgt tgcttacaaa atcctttgct atgattcgca
     3601 ggaagcgcga agaagagaag gtctccccga aggtgcagaa aagccgccgg gacctcctgt
     3661 tagaatcgaa gacccggcca cttacaaagg cggatgcgaa gaagaaggag aaagctctgt
     3721 cttctttctc ttcaatatag tgccggtcac tccccctctc aatcccgagt atgcgattgg
     3781 aactgcggtc caaaagctag agggagacac gatgattgga atgaagacct ggcaggagac
     3841 tcactactat tccatcctgg gtcgcgtgtc cgtcttcaaa gtgaaaggtc ttgtcatcaa
     3901 gtttgataac aaagaaccaa cagattcaaa atctgattcc aaagacaaag acaagaaggg
     3961 agacaaagcc acagacaaga aagacgacaa aacggataag aaagacctca aagcagatca
     4021 gaagaaatga aagtcatcgc atttgctgta ctgctgctcc tctcagggtg cattgttgaa
     4081 aatgacactg tagatactgc agacgcgtac tccgaaattt acgcttccat tcaattcaaa
     4141 gcaagacaat gcggcaatca accggcctat cctcttctgg tgccagaaaa tccacccagt
     4201 tacggaacca gactctgttc tctttccatc attcgccagg attgtccttt tacaaattac
     4261 ccgcttttct gcgttgaaat gttcggcatc gatctgccag ggatcggtcc gtgatgaagt
     4321 ggcgcgcgct tcttgttttc tttctgcttt ttccagcgac tgtatttgct atcgacgtaa
     4381 agatcaagat cgaagacaaa aaggcgggca aacctgtttc agatgcgcag gtaatcgtca
     4441 tggaaacaaa agaaaagttt ttctctgacg aaacgggaga agcaatcatt cgcatccccg
     4501 caaaaggatt ctacaccatc cgggtgattt caccgtcagg cgaaattgtg cagcctcgtg
     4561 tggaagtcat cagccccaat cagacagtga cgatcttcct tggaattgaa gaagaaaagc
     4621 caatacctac aaataccccg cagaaggtaa ctgcggaaga aggaatcagc gttacaggta
     4681 aaagagaaag gcagaaagtt tcgcgttacc aggtgcgcat tgatgaagtg aaacgaattc
     4741 cgggccagtt cggggaagcg ctgagaggtc tcgaaacact gcctggcgtc aatgctccgc
     4801 cttttgggaa cggagacata gcagttagag gagcaaacac aaattcgaat a
//
LOCUS       DAORFF010000278         4847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9930_length_4847_cov_4.056135, whole genome shotgun sequence.
ACCESSION   DAORFF010000278 DAORFF010000000
VERSION     DAORFF010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9930_length_4847_cov_4.056135"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PKK76_11620"
     CDS             <1..540
                     /locus_tag="PKK76_11620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNL69168.1"
                     /translation="PEGIVGLDIEDLQRVRRSAENTLSDGAYHAIRLFWHRISIYHLT
                     PRGRPLLTGIGFISSGFGQRPDPFGNPTGETHTGMDFAAAPKSPIVATSPGVVMQVSK
                     SDRPGYGLFVRIHHGLGYTTLYAHCDSLLVNVGDRVQRGQPIALLGRTGRATGHHVHY
                     EVRFGDDKAIDPLEYVTAR"
     gene            complement(555..1277)
                     /locus_tag="PKK76_11625"
     CDS             complement(555..1277)
                     /locus_tag="PKK76_11625"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL69169.1"
                     /translation="MARVQIIGSGNAFNMDGRAHACYLIESDLLLDCGASSVLRMQSL
                     HVDLSFIAGVLFTHFHGDHLAGLPFLLLNYKYILKRSESLTILGPRGIQETCERLLEL
                     TYPGMTFEFPIHYFEIIPGRDVAFRDKTIRGMPVTHRPESLGYRITWEKDSSRRTFAF
                     SGDAAYDSQLFELVRGVDLAVVELSLVENDGSVAHVALSEVRDGSLRSQRVVYSHVYD
                     ELANLARMEGLTVAEDGMILEF"
     gene            complement(1280..2779)
                     /locus_tag="PKK76_11630"
     CDS             complement(1280..2779)
                     /locus_tag="PKK76_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69170.1"
                     /translation="MRFRSLMVALIIPVAVVVCDRLLFDRVLFSLSNPSGWDSFRWYN
                     FESVFRDVERRRSSDTRPLIVAVGSSIAQYSILPREMENHLGGKYHVELISHAAMLPT
                     DLLNYRKRIAALKPAAVVWTTNPVDLDLERLTPIWEAGPYRSQRTERNFVSRRIPARL
                     YYPDSIMRESEIGAELTSAYAVRTALYSARYPRDEWLPFVQFVSDWNGPLKSYLNYQG
                     IPIAGGIFREGFTGACFGLSEEVTGKEIWMEFPSALYSAGVRLVVRPLRAAEMATCDS
                     FSGTEIVPSRAGWQQITLPPGKLEIRLTHVMVEGSVKEMQSGPAFQGRGVRLPGNLGR
                     REPATQEVYLRRPNLEEARFESMSERELILDYENRMNPPDWRLRPPLVNMGTIRLSKT
                     LVAYAGFVETRQMADMKHFITELAADVPVLIVNQAEHPLSLDEYVWSQWYADYVGFIQ
                     AMKSSRVSVLDLHDALPMRELGDPHHLTFKGARHVQPMMADALSKLLGK"
     gene            complement(2776..3897)
                     /locus_tag="PKK76_11635"
     CDS             complement(2776..3897)
                     /locus_tag="PKK76_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69171.1"
                     /translation="MNLIRKALVRSLPLSMRVKSALQENGCRVRFDFESRDWSSDISS
                     FLFDDGSALRSADVTNAPRDPIKTLSETGYFSKTELLYLEAVENDPDDAGRITLYWPA
                     ERTSLERWIAGSSRVHPSALLPAKEAGKIESIEMPSESAEPGISAPATEVTQTAPSDP
                     ITDQKDPLRIADEKDRKKPAPPAREAQVPSRPVLTPWGTMRYGVVGGLRGTTFRRSYI
                     LHRTDRMCFAMMAGEKSAVQTSQGPFIEYLKKMDDWPLAHQSYSQKHPQQMETISIEI
                     FSDGHCRFFCDGIQAVYWSGRLKKLMRFPNTQAPLKSRLHSGDFVLLIPGGWNSREAA
                     EMREIFLYQESDLAERLSALLDYSARGKGVLLQGPEGVP"
     gene            complement(3899..4345)
                     /locus_tag="PKK76_11640"
     CDS             complement(3899..4345)
                     /locus_tag="PKK76_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNL69172.1"
                     /translation="MQETAHDYDLMKLGFEGERAITADLFFKYGRTYKPKEIILKEGD
                     KGSEVYLIISGKVVVTERIEKGSYRVLNALGPGEIFGEMAMLENAPRSATLIAAVDTK
                     LLCLTPENFEKIFKTHPRWAFKILVALGRRIQAAFTTVEQHFGVRK"
     gene            complement(4347..4754)
                     /locus_tag="PKK76_11645"
     CDS             complement(4347..4754)
                     /locus_tag="PKK76_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNL69173.1"
                     /translation="MDHQTYDRPSLETIKGIGRPMTFEAGQKIYQDAYLVGDPCAYLI
                     VEGEVELVKAYTPLQKDVFRFGKGDVFGMLEVYVNTARMTDARAATQVQVIGFSRVEF
                     ERAMAANLTFALTCIRILSKMLRQINLKLKQMP"
BASE COUNT         1196 a         1298 c         1190 g         1163 t
ORIGIN      
        1 cctgaaggaa ttgtggggct ggatatagaa gacctgcaga gagtacgtcg ctctgctgaa
       61 aatactcttt ctgacggcgc ctatcacgcg atccgattgt tctggcatcg catttccatt
      121 tatcacctga cgccacgcgg cagaccgctt cttacgggaa ttggttttat cagctctgga
      181 tttggacagc ggccggatcc attcggaaat cccacgggtg aaacacatac gggaatggac
      241 tttgcggccg caccaaagtc gcctatcgtt gcaacgtcac ccggcgttgt gatgcaggta
      301 tccaaatcag acagaccggg ctatggcctg tttgttcgca tccaccacgg ccttggatac
      361 acgactctct acgcgcactg cgattctctt ctagtgaatg taggagaccg tgtccagaga
      421 gggcaaccca tcgcacttct cggaagaacc ggccgagcta cagggcatca cgtgcactat
      481 gaggtgagat tcggcgacga caaagcaatc gatccactcg aatacgtcac cgctcgctag
      541 tgaaacttcg aaaatcaaaa ctccagaatc attccgtcct ctgccacggt tagaccttcc
      601 attctggcaa ggttcgcgag ttcatcgtaa acatgagaat agacaacgcg ctggctgcga
      661 agagacccgt cccgcacttc gctcagtgca acatgcgcca cacttccgtc attttccacc
      721 aggctcaatt ctacaactgc gagatctact ccgcgcacaa gttcgaatag ttgtgaatcg
      781 taagccgcgt cgccagaaaa agcaaaggtc cgacgggagg aatccttttc ccacgttatg
      841 cgatatccga gactctccgg tctatgagtc acgggcatcc cgcgaatggt tttgtccctg
      901 aatgctacat ctctaccggg gataatttca aaatagtgga tgggaaactc gaaagtcatt
      961 ccaggataag tcaactcgag taacctttcg cacgtttcct ggattccacg cggacccaga
     1021 atggtaaggc tctctgatct ctttagaatg tatttgtagt tcaatagcaa gaacggaagt
     1081 ccagcaagat gatcgccgtg gaaatgagtg aacagcactc ctgcaataaa agatagatcc
     1141 acgtgcagag actgcatgcg aagaacagaa gaagcgccac agtccaaaag aagatccgat
     1201 tctatcaaat agcaggcatg ggcccgacca tccatgttga acgcattgcc ggatccaatg
     1261 atttgaaccc gggccattcc tattttccca ggagtttcga aagagcgtcc gccatcatgg
     1321 gttggacgtg acgagcacct ttaaacgtaa gatggtgcgg atccccgagt tcgcgcatgg
     1381 gaagagcatc gtgcaaatcg aggacagaca cgcggctaga cttcattgcc tgaatgaagc
     1441 caacgtaatc cgcataccac tggctccaga cgtattcatc gagcgaaagt ggatgctctg
     1501 cctgattgac aatgagaact ggaacatccg cggccagttc cgtaatgaaa tgcttcatgt
     1561 ccgccatctg ccgagtttcc acaaagccgg cgtatgcaac cagagtcttg gataagcgaa
     1621 tcgtgcccat attcacaaga ggtggacgca acctccaatc gggaggattc attcgatttt
     1681 cgtaatcaag gatgagttca cgttcgctca tggattcaaa gcgagcttct tccaggttgg
     1741 gtcttcgcag atatacttcc tgagtggcag gctcacgccg cccaagattc cctggaaggc
     1801 gaacacccct tccctggaaa gccgggcctg actgcatctc ttttacactt ccttcgacca
     1861 tcacgtgagt cagtcgaatc tcaagtttgc ctggaggcag agtgatttgc tgccaaccag
     1921 cgcgagacgg aacaatctct gttccagaaa aagaatcaca cgtcgccatt tctgcggcgc
     1981 gcagtggacg cactacaagc ctcactcctg cagaatagag agcggacgga aactccatcc
     2041 agatttcttt tccagtcact tcttcgctca gaccaaagca ggcgccagtg aatccttctc
     2101 tgaagatccc acctgcaatg gggattccct gatagttgag ataactcttg agcggtccgt
     2161 tccaatcaga tacaaattga acaaaaggca gccattcgtc gcgcggatag cgagcggaat
     2221 aaagcgcagt cctcaccgca tacgcactcg ttagctctgc gccaatctcc gattcgcgca
     2281 tgatgctgtc aggatagtag agcctggcgg gaatccgacg cgaaacaaag tttctctctg
     2341 tacgctgcga cctgtatggc cctgcttccc agatcggagt taaacgctct agatccagat
     2401 ccactggatt cgtggtccag acgacggcgg ccggtttgag tgctgcaatg cgtttgcgat
     2461 aattcagcag gtctgtgggg agcatggctg catgcgaaat gagctccaca tgatatttgc
     2521 ctcccaggtg attctccatt tcgcgcggca aaatggaata ctgagcgatg ctcgaaccta
     2581 ccgcaacgat gagaggtctg gtgtcagagg acctgcgtct ttccacatcg cgaaacacgg
     2641 attcaaaatt ataccagcga aacgaatccc atccggaagg atttgataga gaaaaaagaa
     2701 cacggtcaaa gagcagcctg tcgcacacaa cgactgcaac aggaatgatc aatgcaacca
     2761 tcaacgaacg aaatctcacg gcactccctc tggtccctgg agcaatacac ccttacccct
     2821 ggccgaatag tcaagcagtg cggaaagtct ttctgcgagg tccgattcct gatacagaaa
     2881 aatttcgcgc atttccgccg cttcgcgcga attccatcca cccggaatca aaagaacaaa
     2941 atcgcctgaa tgtagcctgc ttttcagagg tgcctgggta tttggaaaac gcatgagttt
     3001 tttcaaacga ccggaccagt atactgcctg aattccatca cagaaaaatc tgcaatgacc
     3061 atctgaaaat atttcgatgg aaatggtctc catctgttgc ggatgtttct gggaataact
     3121 ctggtgagcg agtggccaat cgtccatttt cttcaaatat tctatgaaag gtccctgact
     3181 cgtttgcacc gcggattttt ctccggccat cattgcaaaa cacatacggt ctgttctgtg
     3241 tagaatgtaa cttctgcgaa acgtggttcc acgaagtccg cccacgacac catagcgcat
     3301 ggtgccccac ggggtgagca cagggcgaga aggcacctga gcttcgcgcg cgggcggggc
     3361 cggttttttc cgatcctttt cgtccgcgat gcgcaaagga tctttctggt ccgtgattgg
     3421 atccgaggga gctgtctgtg tgacttcggt ggctggcgcc gagataccgg gctctgcaga
     3481 ctcggaaggc atttcaatcg attcgatctt gcccgcttct ttcgcaggca agagagcaga
     3541 aggatgaaca cgtgaagaac cggcgatcca tctctcaagg ctcgttcttt ccgccggcca
     3601 gtacaacgtt atgcggccag catcgtctgg atcattttct acggcctcca gatagagcag
     3661 ttctgttttt gaaaagtatc ctgtttcgct gagagtcttg attggatcgc gaggagcatt
     3721 cgttacgtcc gcactgcgaa gagcgcttcc atcatcaaat agaaacgaag atatatcgct
     3781 ggaccaatct ctggattcga aatcgaaccg cacgcgacag ccgttttcct gaagcgcact
     3841 cttcactcgc atggacagag gaagagatcg cacgagcgcc ttcctgatca gattcatttc
     3901 actttcttac gccgaaatgc tgctccacgg ttgtgaatgc agcttgaatg cgccggccaa
     3961 gcgctactaa aatcttgaac gcccatctgg gatgagtttt gaagatcttc tcaaagtttt
     4021 caggagtcag gcagagcaac ttcgtgtcca ctgcggcgat caaagtggct gatcgcggag
     4081 cgttttcgag cattgccatt tccccgaaga tttctcctgg cccgagtgca ttgagcacgc
     4141 gataagagcc tttttcgatt ctttctgtta caaccacttt gcctgaaatg atcaggtaca
     4201 cctcggagcc tttgtcccct tctttgagga tgatctcctt gggtttgtag gttcttccat
     4261 acttgaagaa taaatccgct gtgatggcac gctcgccttc aaagccgagc ttcatcaaat
     4321 catagtcatg agctgtttct tgcatatcag ggcatctgct tcagtttgag gttgatctgc
     4381 ctgagcatct tactcagaat tcgaatgcat gtaagtgcga aagtgagatt ggcagccatg
     4441 gcccgctcga actcgacgcg cgaaaatcca atgacctgca cctgagtggc ggcacgcgcg
     4501 tccgtcattc gcgcagtgtt cacgtatacc tctagcattc caaaaacatc gcctttgcca
     4561 aagcgaaaca cgtctttctg aagaggagtg taggctttta caagctccac ttctccttct
     4621 acaatcaggt acgcgcaagg gtctcctacc aggtatgcat cctgatagat cttctggccc
     4681 gcttcgaaag tcatgggcct gccaatgcct ttgatggttt ccagggaggg tcgatcgtag
     4741 gtctggtggt ccatacgtgc tacagcttcc ttttatcctt gctttgcttt cccgtccagc
     4801 aattcacgtc tggagatgcc cgttaccttt gacgagtgct tccagac
//
LOCUS       DAORFF010000279         4838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9962_length_4838_cov_4.199247, whole genome shotgun sequence.
ACCESSION   DAORFF010000279 DAORFF010000000
VERSION     DAORFF010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9962_length_4838_cov_4.199247"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PKK76_11650"
     CDS             complement(<1..778)
                     /locus_tag="PKK76_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69174.1"
                     /translation="MYDICRVPYYKISDPLFRGSMMLEYNYQASKGSTGNPTPVQYGT
                     LGGANDFYGSGGLLAPNGKIYAPPNDQNAINNQALIIDPKTNITSTIAGIGGAGPKWS
                     PGALAANGKMYFFPISGTTSILSIDPTTNALSTFGSVTAGYGGAVLAPNGKIYAIPMT
                     AGLPVAVFDPSTNQFTSISGATTGGWIGGVLALTGKIYAFPMGNIAAVGFLEIDPTND
                     SVSTFASFAGTQGFFGGGMAPDGRFYSLPVGNHQSMVFDPF"
     gene            complement(979..1953)
                     /locus_tag="PKK76_11655"
     CDS             complement(979..1953)
                     /locus_tag="PKK76_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNL69175.1"
                     /translation="MNVVILLVPDAPASVVTGLSEFFEIAGLDMNERRAKRPCRVRTA
                     SVQADRVPLRGKVIIEPDIVGRMTEPCDLVIVPALGPNVAGIRRRLGMEIEWLKDAAS
                     RGTRIASVCSGAFLLASTGLIDDREATTHWLFAPLFRRMFPRVKLNIDRLVIDTGKFL
                     TSGGSNAFYDLALHVIEQSLGRDTALSCARHFLLDTNRVSQTPFIAFSAQKHHGDAGI
                     GAAQTILEKEFSTALSIDTIADRVGMSPRNFKRKFKQVTGDAPSVYLQRLRIEWARNR
                     LAETGDSIESISYAVGYENVGFFRLLFKRHTGLTPFDYRRKNSRSSRN"
     gene            2111..2509
                     /locus_tag="PKK76_11660"
     CDS             2111..2509
                     /locus_tag="PKK76_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HNL69176.1"
                     /translation="MLLAARIIAGIVGLLHVYIFMMESVWWMRPAIFKRFGVPDAKQA
                     EIMKSVFLNQGFYNLFLAIGALYGAICFELHPMYAPAIMIFACLSVFGAGTVLLVSAG
                     KRMLRAAIMQGLPPAISLALLASVLLRTAG"
     gene            2581..3234
                     /locus_tag="PKK76_11665"
     CDS             2581..3234
                     /locus_tag="PKK76_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNL69177.1"
                     /translation="MQTVLITGANRGIGRATADALVARGCKVYYAARSIENLPSGDLA
                     TPISLDVTDPTSIARAVVFFEERKEALDVLINNAAIIGRADVTSFDVEEITRVMDTNF
                     NGPVRVTKAFFPFLEKSPSARVIHVSSGMGAISDLAGDHAAYRFSKSALNAFTISLAR
                     AQDRVKVVAVCPGWVRSDMGGKSAPRSLEEGAVGIVGLALNQIESGKFYRDGRVIPW"
     gene            complement(3251..4672)
                     /locus_tag="PKK76_11670"
     CDS             complement(3251..4672)
                     /locus_tag="PKK76_11670"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2219 family protein"
                     /protein_id="HNL69178.1"
                     /translation="MRVTQRLLLTVIALAADTSLRGEIPLEPITRPFPYVGRFSWDND
                     APLGSDREYTSGLRLDAGSYDFLYLPPSFLLHALTSITDSPGGEYSGISASQFMFTPK
                     NLIRSDIAYGERPYSGVAQASMLSAMWWENTAISTEIGAGEIGPKSQAGSSQRKFHQV
                     IRSTLPEGWGNQIPTQKFFQWNSNIKYSISSFMAIDLTGRVGGLDSSVSAGPLFRFGS
                     APNTPAQGTAIYDDPSPLAPPAESETYVYIEPSIKYQGRNGTLAGGSRGLSTSDLRTL
                     VSNVSPNSIIASPLYGQISNEHTGSDIRRFVAFQTAVNQEPSIGWNYLIFNSIFNGGP
                     TIDRDVRDAILRDILTSGMNAADYPLAPFFAYDTLFRKPQKPQSVLTKYLAYVYLFQG
                     RPLSAENLVAATALLWNERDGSRRYAVQPRHVQGRFSIGMVFNRPGWFLQAGATISSL
                     EYHSDSGVTPFHRYATLQFGTRF"
BASE COUNT         1150 a         1191 c         1281 g         1216 t
ORIGIN      
        1 cgaaaggatc aaaaaccata gactgatgat ttcccacagg cagggaatag aaacgaccgt
       61 caggcgccat tcctcctcca aagaaaccct gggtgcctgc aaaacttgcg aaagtcgaaa
      121 ccgaatcatt ggttggatca atctctagaa agcccaccgc ggcgatgttt cccatcggaa
      181 aggcgtatat tttccctgtt aaggccagca ctccgccaat ccaacctccg gtcgttgcac
      241 ctgatatgga cgtgaactgg tttgtagatg gatcaaatac tgctacaggt agtccggccg
      301 tcataggtat cgcatagatt tttccattgg gcgcaagcac tgcaccgccg taacccgccg
      361 taacacttcc aaatgtactg agagcatttg tggtaggatc gatcgagagg atgctggtgg
      421 tgccactgat cgggaagaag tacattttgc cattggcagc aagagctccc ggactccatt
      481 ttgggccggc gcctccaatg ccggctatcg tggatgtgat gtttgtcttt gggtcaatga
      541 tgagagcttg attgttgatt gcattttgat cattgggagg ggcgtagatt tttccattag
      601 gcgccagcaa tccacctgag ccataaaagt cattggcacc tcccagtgtg ccgtactgta
      661 caggcgttgg atttccggta gatcctttgg atgcttgata gttatattcc agcatcatgc
      721 ttccgcgaaa gagtggatcc gagatcttgt aataggggac gcggcagatg tcgtacatga
      781 tcaggttggc cattagcttg caggatacgt ccgtgtcccc acattgctgg tgctgcgagc
      841 accccgtgcc cgaaagcgtg aataagacga agccgatcgc gcataacgtt cgccggacgg
      901 ggcatcgcat agcgcatctg aagtgaacct gtaggatctg aaaagaagaa tcctggtttt
      961 gcgacaaagg agtcgcgttt aattgcgcga agacctggag ttttttctcc tgtagtcaaa
     1021 tggagtcagg cccgtgtgtc tcttaaagag gagtcgaaag aatcctacgt tctcatatcc
     1081 cacggcgtag ctgatgcttt caatggagtc tccggtttcc gccagtctgt ttctggccca
     1141 ttcaatgcga agccgttgca aatagactga gggcgcgtct cccgtcacct gcttgaattt
     1201 tctcttgaaa ttcctgggac tcatgcccac tcgatctgca atcgtatcaa tagagagagc
     1261 agttgagaat tccttttcga ggatcgtctg cgcagcgcca attcctgcat ctccatggtg
     1321 cttctgagcg ctaaatgcaa tgaaaggtgt ttgagagact ctgtttgtat ccagaaggaa
     1381 gtgtctcgcg cacgaaagag cagtgtctct tcccagggac tgttcaatca cgtgaagggc
     1441 cagatcgtag aaggcattgc ttccgcccga cgtcaggaat ttccccgtgt ctatgaccag
     1501 tctgtctatg ttcaatttca cacgggggaa cattctgcga aataaagggg caaacagcca
     1561 gtgagttgtt gcttctctgt cgtctatgag gccagtggat gcgagcagaa acgctccaga
     1621 acaaacactg gcaatgcggg tgccacgact ggccgcgtcc ttcagccatt cgatttccat
     1681 gcccagtctt ctcctgattc cggccacatt cgggcccagg gctggcacga tcaccagatc
     1741 gcaaggttct gtcattcgtc ctacaatgtc aggctcgatt atgaccttcc ctctcagtgg
     1801 aactctgtct gcctggacgg atgcggtcct gacccggcaa ggtctcttcg ctcttctttc
     1861 attcatgtcc agcccggcga tttcgaaaaa ctcggacagt ccagtgacca ccgacgcagg
     1921 cgcatccggc acaagcaaaa tcactacatt cacgctccga tcgaggcatc ccggtggcgg
     1981 attacaactg tttaatgcga ttaggccgaa taattgatgg cataatcacg atgaaaattg
     2041 gcatcaatcg agctagcatc ctgtgccact ctgtggtagg atgagctcat catttttcag
     2101 gagaactcag atgttattag cagccagaat catagcgggc atcgttggat tgcttcacgt
     2161 atacattttc atgatggaaa gcgtgtggtg gatgagacct gccatcttca aaagatttgg
     2221 ggtgcctgac gcaaagcagg cagagatcat gaagagcgta tttttgaacc agggatttta
     2281 taatctattt ctcgcaattg gcgctctgta cggagccatc tgttttgaac tccatccgat
     2341 gtatgcgccg gcgatcatga ttttcgcatg tctatctgtg ttcggcgcgg gcactgtgct
     2401 cctggtttct gcgggcaaaa gaatgctgag agcggcgatc atgcaaggcc ttccaccggc
     2461 gatttcgctt gcactgcttg cctctgtgct tctaagaacc gcaggttgat cctgggtcct
     2521 gcgagggcgc aagtgcgcct tcgtatggac aatcgctttc gtttgtgttt tattctttga
     2581 atgcaaacag tactcatcac aggagcaaat cgaggaatag gtcgagccac agcggacgct
     2641 ctggtagcac gcggttgcaa agtgtactat gcggccagaa gcatagaaaa cctaccgtca
     2701 ggcgatcttg ccaccccgat atcactcgat gtaacggatc ccacgtccat tgcgcgcgca
     2761 gttgttttct ttgaggaaag aaaagaagca ctcgatgttc tcatcaacaa cgccgccatc
     2821 ataggaagag cggatgtgac ttcttttgac gttgaagaga taacacgagt catggataca
     2881 aatttcaacg gcccggtgcg tgttacaaaa gcattctttc cctttctgga gaaatctcct
     2941 tccgcgcgcg tcatccatgt ttcgagtggg atgggcgcta tctctgatct tgcaggcgat
     3001 catgccgcct atcggttttc caaatctgcc ctgaatgcgt ttactatttc tctggctcgc
     3061 gctcaggacc gggtaaaggt tgttgcagtc tgtccgggat gggttcggtc agacatgggt
     3121 gggaagagcg cgccgcgcag tctagaagaa ggtgctgttg gaattgtcgg tcttgcactc
     3181 aaccagatcg aatcaggtaa attttaccgc gatggtcggg tgattccctg gtaaacgttt
     3241 gtgtcagctt ctagaaccgc gttccaaatt gcaatgtcgc ataacggtga aacggagtaa
     3301 cccctgaatc agaatgatat tcgagagagc ttatggttgc gccagcttgc agaaaccaac
     3361 ccggccgatt gaataccata ccaatagaaa atcgaccctg aacatgccgc ggctggactg
     3421 cgtatctcct ggagccatcg cgttcattcc agagaagagc ggtggcggca accagattct
     3481 ccgccgacaa tgggcgtccc tggaagagat aaacatacgc cagatacttc gtcaggacgg
     3541 attgtggttt ttgaggctta cgaaatagag tgtcgtacgc gaaaaaaggt gccagcggat
     3601 aatcggcggc attcattccg cttgtgagta tgtcgcggag gattgcgtcg cgcacatctc
     3661 tgtcaatcgt tggcccgccg ttgaaaatag agttaaagat gagatagttc cacccaatgg
     3721 atggttcctg gttcactgcg gtctgaaatg cgacaaagcg acggatgtcg cttcccgtgt
     3781 gttcgttcga gatctggcca tagagtggac tggcgatgat ggagtttgga gacacattcg
     3841 atacgagcgt cctcaaatcg gaagtggaga ggccgcgcga acctcctgcc agggttccgt
     3901 ttcgcccctg gtacttaatc gatggctcaa tatagacgta cgtttcggac tcagccggag
     3961 gtgccagtgg agaaggatca tcatatatgg cggtgccctg ggcgggagtg ttgggtgctg
     4021 acccaaatcg gaagagtggt ccggcagaaa cggaagaatc aagtccaccg actcgtcccg
     4081 ttaagtcaat ggccataaac gatgatatag aatatttaat attagaattc cactgaaaga
     4141 acttctgcgt tggaatttga tttccccagc cttcggggag cgtggagcga atcacctggt
     4201 ggaactttct ttgggaactg cctgcctgcg attttggacc aatttcacct gcgccgattt
     4261 ccgtgcttat ggctgtattt tcccaccaca tggcagagag catgctcgcc tgggccactc
     4321 cgctgtaggg acgttcgccg tatgcgatgt ctgatcggat tagatttttt ggagtgaaca
     4381 tgaattgcga tgcggatatg ccggagtatt ctccccctgg cgaatctgta attgaggtga
     4441 gggcatggag caggaaagaa ggcggcagat aaagaaaatc gtagctcccc gcatctaacc
     4501 gaagtccgct tgtgtattcg cgatcggatc caagcggcgc gtcgttatcc caggagaatc
     4561 ttcccacgta cggaaaaggc ctggtgattg gctctaaagg aatctctccc cgcagggaag
     4621 tgtctgcagc cagagcgatg actgtcagca ggagcctttg tgtcacacgc atgattcagt
     4681 acaaactaaa ttgcagcagt ctgcattcaa ttggtccatt aaaaaaaatg gtcttcttgt
     4741 ccgccttgag tccaacttct tttgaaagtg cgacagaacc cgtaaaaacg aaaccttccc
     4801 atcccttgaa cttctgctta tagagatcgc cgatctta
//
LOCUS       DAORFF010000280         4836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_9972_length_4836_cov_3.322736, whole genome shotgun sequence.
ACCESSION   DAORFF010000280 DAORFF010000000
VERSION     DAORFF010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9972_length_4836_cov_3.322736"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PKK76_11675"
     CDS             <1..1021
                     /locus_tag="PKK76_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNL69179.1"
                     /translation="GNGKKLVIRRARENNLKGIDVEIPLGKLIAVTGVSGSGKSTLVN
                     DILYEALAAKIGEGRHHPGKHDRITGVEALDRVICIDQEPIGRTPRSNPATYTGLFTP
                     IRDLFSRLPEAKVRGYAPGRFSFNVAGGRCENCEGAGIITIEMHFLPDVYVTCDVCKG
                     KRFNRETLEVKFHGKNIHDVLDLTVDDGVEFFRNIPLVARKLETLQEVGLGYIRLGQA
                     ATTLSGGEAQRVKLATELSKKSTGRTLYILDEPTTGLHFEDISRLLSVLHSLVERGNS
                     MVIIEHNLDVIKTADHLIDLGPEGGDEGGQIIAEGTPEEVAANPASHTGKYLGQILAR
                     GRKSA"
     gene            1021..1830
                     /locus_tag="PKK76_11680"
     CDS             1021..1830
                     /locus_tag="PKK76_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNL69180.1"
                     /translation="MWQSPEISAQEFAQYQRLLYELTGISLNDTKKPLVASRLLPRLR
                     HYGMKSFSEYLRLVKTDPNESQHMTDLLTTNETRFFREDAHLAFLKSELQRTSGQGKS
                     YSLWSAASSSGEEAYSMAMICAEVLGVNGSFQILGSDISQRILDRATRAVYAMDRAAT
                     IPLDLLKKYCLRGVRSNEGTFTIIPELKRHVEFRQVNLLEIPRSLGSFDFVFLRNVMI
                     YFDQPTRSRIIESIAPLLSRDGCLIIGSAESLLGITTSLVSAGPNVYRRKQ"
     gene            1827..2873
                     /locus_tag="PKK76_11685"
     CDS             1827..2873
                     /locus_tag="PKK76_11685"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNL69181.1"
                     /translation="MKKIGVMIVDDSAVVRTVLTTVLTSVSDIEVIATAGDPIFAMEK
                     MKKRWPDVIVLDIEMPRMNGIDFLKTLMRDHPTPCVICSSLTAEGTSVAMQAMAAGAV
                     SIISKPKMGTKEFLEESRDRIVDAVRGASQSQLKPIDAQDLPPRAPEPAADKPAVHTS
                     YKRMVVLGASTGGTMAIEQVLSGLPADCPAVLIVQHMPEKFTHAFADRLDKSMRMSVR
                     EAQNGDPIMPGRVLIAPGNQHMEIKRTATGLEAVVRSGDYVNRHRPSVDVLFESAAKL
                     GGTSFIGVILTGMGGDGSQGLLSLKDKGARTIAQDQATSVVFGMPQEAIKLGAAELVL
                     PIDKIAAEILKLAR"
     gene            2962..4716
                     /locus_tag="PKK76_11690"
     CDS             2962..4716
                     /locus_tag="PKK76_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNL69182.1"
                     /translation="MIQNNYFNTNEDLKKQFSDIIDWKEIVDAFENGFADAEEYKKTS
                     NEHLQYAPSNYEDAIEYYRTLLENLGDITGTTVASLAAEMDRIGLKYDSGNVTFPDAM
                     IKAYKAVQETGLQPYGISRRRGGLGIPCTVQALMMELLGRADASLAIALGCANLAETI
                     ERFASKEMSDKYVPLMASGELTGAMALTEPNYGSDLPGVQTKAEKGADGIWRLTGTKR
                     FITHACGFAGTPSVILTLARTGSPTSGARGLSFFLVHGKDVHVASIEKKMGLHCSPTC
                     EVVYDNSPGELIGEESQGLVKYAMGMMNTARLSIAAQAMGIGSAAYFEAKKYASERVQ
                     FGKTIDKIPAVRKMLDLMERETAAMRCLLIEASRTVDMYLWRLERLEHSGLPDREVRK
                     DEQVRKWEKLANYFTPLSKYYTSEMCNKVAYDAVQIHGGSGYTEDYDVSRIFRDARIT
                     NIYEGTTQLQVVASIGGVVAGMAPAGHLRAYLDEEMAKFQPSAELTQIRATLEDAITA
                     YKSISDGDSRDMLSFELVEMSARLIAGMLLERAASRQQGADRADRLRIAKLYNSDSQA
                     ICAANTTKIKNAGSLVTA"
BASE COUNT         1315 a         1229 c         1252 g         1040 t
ORIGIN      
        1 aggaaacgga aagaaactgg tcattcgtcg cgcgcgcgaa aacaacctga aaggcatcga
       61 tgtggaaatt ccactcggca aactgattgc agtgaccgga gtttccggat caggaaagtc
      121 cactctggta aacgacattc tttacgaagc gcttgcagcc aaaatcgggg agggccggca
      181 ccatccaggg aagcatgatc gcataacggg tgtagaagca ctcgaccgag tcatttgcat
      241 tgaccaggaa cctattggca gaacacccag atcaaacccg gccacataca ctggtctttt
      301 tactcccatt cgtgatctct tctcacgcct tccagaggcg aaagttcgcg gatatgctcc
      361 cggcagattt agctttaacg ttgccggcgg aagatgcgaa aactgcgaag gggcaggcat
      421 catcacaatt gaaatgcact tccttccaga tgtctacgta acgtgcgacg tatgcaaagg
      481 aaagcgattc aatcgagaga ccctggaagt gaagttccac ggcaagaaca ttcatgatgt
      541 tctcgacctg accgtggacg atggagtgga attctttcga aacattcctc tggtggcgcg
      601 caaactcgaa actctccagg aagtgggcct tggttacatt cgacttggcc aggcagccac
      661 tactctctct ggcggagaag cgcagagagt aaaactcgcc actgagctct ccaagaagag
      721 tacgggaaga actctctaca ttctggacga accaaccaca ggccttcatt tcgaagatat
      781 atccagactc ctcagcgtac ttcattctct cgtagaaaga ggaaattcca tggtgatcat
      841 cgaacacaac ctcgacgtca tcaagactgc agatcacctc atcgaccttg gaccggaagg
      901 tggagacgaa ggcgggcaga tcatcgcaga aggaacgccc gaagaagtag cggccaatcc
      961 agcaagccac actggcaaat acctcggaca aatcctggct cgtgggagaa agtcagcctg
     1021 atgtggcaaa gtcctgaaat ctctgcgcaa gaatttgcgc agtatcagag actcctgtac
     1081 gagctgacag gcatttcctt gaatgataca aaaaagcctc tcgttgcaag caggctacta
     1141 cccaggttgc gccactatgg gatgaaatcc ttcagcgaat atctgaggct tgtcaaaacg
     1201 gatcccaacg aaagccagca catgacggat cttctgacga cgaatgaaac cagatttttt
     1261 cgcgaagatg cgcatcttgc tttcttaaaa agcgagctgc aaagaaccag tggccagggc
     1321 aaaagctatt ccctgtggag cgccgcctcc agctccggag aagaagccta ttcgatggcc
     1381 atgatttgtg cggaagtgct cggggtgaat ggctcctttc aaatccttgg aagtgacata
     1441 tctcaaagaa ttctggaccg agccacaagg gcagtttatg cgatggatcg tgccgccacg
     1501 attccgctcg atctcttaaa gaaatattgt ttgagaggag tgcgcagcaa cgaaggaaca
     1561 ttcacgatca ttcccgaact caagcggcat gttgaattca ggcaggtgaa tctgcttgaa
     1621 atcccaaggt ctctgggttc ttttgatttc gtgtttctgc gaaatgtgat gatctacttc
     1681 gatcagccaa ccagatcacg cattatagaa agcattgcac cattgctttc cagagacggc
     1741 tgcctgatca ttggatcggc tgaatctctg ctgggaatta caacgtcact cgtcagcgcc
     1801 ggaccaaacg tttatcggag aaagcaatga agaaaatcgg cgtgatgatt gtggatgatt
     1861 ctgccgttgt tcgcacggtc cttaccactg tactcacttc tgtctctgac attgaagtca
     1921 tcgcaactgc aggtgatcca atctttgcga tggagaagat gaagaaaaga tggccggatg
     1981 tcattgttct cgacattgaa atgccgcgca tgaacggaat tgatttttta aaaactctga
     2041 tgcgcgatca tccaactccg tgtgtcattt gctcttctct gactgcggaa ggaacttctg
     2101 tcgccatgca ggcgatggcc gcaggtgcgg tttccattat ttcaaaaccg aagatgggaa
     2161 caaaggaatt cctcgaagaa agcagagatc gcattgtgga tgcagtccgt ggtgcctcgc
     2221 aatctcagct caagccaatc gatgcacagg accttcctcc cagagcaccg gaaccagcgg
     2281 cagataagcc ggcggtacac acaagctaca aaagaatggt tgtgctgggc gcatccaccg
     2341 gtggaaccat ggccatcgaa caggtgttaa gcggactgcc ggctgattgt ccggcggttc
     2401 taatcgtgca gcacatgcca gaaaaattta cacatgcttt cgcggatcgc ctggacaaga
     2461 gcatgcgaat gtctgtgcgc gaagctcaaa atggagatcc aatcatgcct ggcagggtgc
     2521 tgattgctcc gggaaaccag catatggaaa tcaaaagaac tgccacgggt ctggaagcag
     2581 tcgttcgcag cggggactac gtgaatcgcc acaggccaag cgtggatgtt ttgtttgaat
     2641 ccgcggcgaa gctgggaggg accagtttca tcggtgtcat tcttacaggt atgggtggag
     2701 acggttcgca aggactgcta tcgctcaaag acaagggcgc gcgtacgatt gcccaggatc
     2761 aggccacatc cgttgtgttc gggatgcccc aggaagcaat caagctggga gccgcggaac
     2821 tcgtgcttcc cattgacaag atagccgcag aaatccttaa actcgcgcga taagcccggg
     2881 cgtgatgcca ttaatcgcaa ttgaccgggt atctgccggt ttttttctcg actgacacgg
     2941 tgttattccg gggagcattg catgattcag aacaattact tcaatacaaa tgaagaccta
     3001 aagaagcaat tcagcgatat cattgattgg aaagaaatcg tagatgcatt tgagaacggt
     3061 tttgcagacg cagaggaata caaaaagaca agcaacgaac atctgcagta cgcaccctcc
     3121 aactacgaag acgcaatcga atactacaga acccttctag agaatctggg cgacattaca
     3181 ggaacgactg tcgcttcgtt ggcagctgaa atggaccgaa tcggtctgaa atatgacagc
     3241 ggcaacgtca cgttcccgga tgcaatgatc aaagcataca aagccgttca ggaaacaggc
     3301 ctgcagcctt acggaatcag ccgtcgtcgc ggcggcctgg gaattccttg cacggttcaa
     3361 gctttgatga tggaacttct aggaagggca gatgcatctc tggccattgc tcttggttgc
     3421 gcgaatctcg cagaaacaat cgaacgattc gcgtccaaag aaatgtctga caagtatgtg
     3481 ccgcttatgg catccggtga actcacaggt gccatggcgc tcacagaacc aaattacggt
     3541 tcagatctcc ctggcgtgca aacaaaagcc gaaaaaggcg cggacggaat atggcgcctg
     3601 actggaacaa aaagattcat cacgcatgca tgcggatttg caggaactcc ttccgtaatt
     3661 ctgactctgg cacgcaccgg atcaccgacc agtggcgcgc gcggactttc gtttttcctg
     3721 gtgcacggca aagatgttca cgtcgcaagc attgaaaaga aaatgggcct tcactgctca
     3781 cctacctgcg aagtagtcta tgacaacagc cccggtgaac taattggcga agaaagccag
     3841 ggtcttgtga agtatgcgat gggaatgatg aacactgcgc gcctctctat cgcagcccag
     3901 gcaatgggaa ttggaagtgc ggcttacttt gaagctaaga aatatgcatc agagagagtg
     3961 cagtttggca agaccatcga caagatcccg gctgtgcgaa aaatgctgga tctgatggag
     4021 cgcgaaacag ctgctatgcg atgcctcctc attgaggcct cacgaaccgt tgacatgtat
     4081 ctgtggagac tggaaagact cgaacacagt ggccttccag accgcgaagt tcgcaaggac
     4141 gagcaagtgc gcaagtggga aaaactcgcc aactacttca caccgctctc caaatactac
     4201 accagcgaaa tgtgcaacaa agtggcgtat gacgccgttc agattcatgg cgggtccggt
     4261 tacacagaag actacgatgt atctcgaatc ttccgcgacg ctcgcatcac caacatctac
     4321 gaaggaacca cacagctcca ggttgttgca tccattggtg gcgttgtggc tggcatggct
     4381 ccggcaggtc acctgcgcgc ttacctggac gaagaaatgg caaaattcca gccgtctgcg
     4441 gagctcactc agatccgcgc cactctggaa gacgcaatca ctgcttacaa atccatcagc
     4501 gacggcgatt cgcgcgacat gctctctttt gaactcgtag agatgtctgc tcgcctcatt
     4561 gctggaatgc ttctggaacg cgccgcatcc cgccagcagg gcgcggatcg tgcggacaga
     4621 cttagaattg caaagctata caattcagac agtcaggcta tctgtgctgc aaacaccacg
     4681 aaaatcaaaa acgcaggttc tcttgtgaca gcgtgatggg aactcccagc atctgaacgc
     4741 aaaaaggccg ggaaaacccg gccttttttt tgaaacaaat cgggatgact ggatttgaac
     4801 cagcgaccgc acgcccccca gacgtgtgcg ctaccc
//
LOCUS       DAORFF010000281         4814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10053_length_4814_cov_3.584997, whole genome shotgun sequence.
ACCESSION   DAORFF010000281 DAORFF010000000
VERSION     DAORFF010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10053_length_4814_cov_3.584997"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PKK76_11695"
     CDS             <1..694
                     /locus_tag="PKK76_11695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL69183.1"
                     /translation="LRDTPAALGLPPVHEYAGESDPFKEAQTQSGREIFINYILKNRI
                     LWIVCIANIFVYIVRIGVFDWAPKFLVSERGFTTKEASVAGFAFEVSGIFGAIAAGWL
                     SDTAFPGRRGAIATVFMVWLAAAIGIVFASPSGNFWLMIFAMVSVGFFVYGPQVLVAV
                     VAAENVDARAVGTAVGMTGFFGYVGATICGVGTGYVVDHFGWDVALVVYLISALIGAG
                     LFAIAWKPVMRR"
     gene            779..1690
                     /gene="deoC"
                     /locus_tag="PKK76_11700"
     CDS             779..1690
                     /gene="deoC"
                     /locus_tag="PKK76_11700"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNL69184.1"
                     /translation="MKSAAVVPDFRYTPVIDQVGVEERVARFSSRSIKKESKVQGLKL
                     ALSMIDLTTLEGKDTEGRVRQMCYKAAHLHDVLPGLPTVAAVCVYPTHVKTAKDALKG
                     TGIKVASVATAFPSGNATLDVKIADTKFAVESGADEIDMVIARGEFLRGNYSFVFDEI
                     AAVKEACGKARLKVILETGELSTLDNVRRASDIAIYAGADFIKTSTGKIQPAATMPVT
                     LVMLDAIKDYYYQTGTMIGMKPAGGISTAKLALQYLVMLYETLGSKWMTNQWFRFGAS
                     SLANDILMQLAKEAHGVYQSPDYFSKD"
     gene            1699..3174
                     /locus_tag="PKK76_11705"
     CDS             1699..3174
                     /locus_tag="PKK76_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNL69185.1"
                     /translation="MATQKKEGPRLDFFSDWKYSPAPESKEHIRLNKKYDLFINGKWQ
                     KPTGSKYFATHNPATEEKLAEVADAGEKDVDAAVQAARSAYEKTWRKMPGRERGKYIY
                     RIARMIQERARELSVIETMDGGKSIRESRDIDVPLAASHFFYYAGWADKLDYAFPNRK
                     PTPLGVAGQIIPWNFPLLMAAWKIAPALACGNTVVLKPAETTPLTALKLAEIIQDSGL
                     PDGVVNIITGAGKTGAAIVNHPHVNKVAFTGSTDVGRIIQKAVAGTAKRVTLELGGKA
                     ANIIFEDAAIDQAVEGIVNGIYFNQGHVCCAGSRLFVQEGVADLVIRKLKDRMETLIV
                     GDPLDKNTDVGAINSKQQLQVITNYIQIGKEGGAEFYQSSCELPKKGYFVRPTLFLNV
                     SQSHRIVQEEIFGPVLAVQTFRTIEEVIEKANNIPYGLSAGVWTDKGSKIFNLTGKLR
                     AGVIWANTYNKFDPTSPFGGYKESGFGREGGVHGLLPYLSL"
     gene            3181..4062
                     /locus_tag="PKK76_11710"
     CDS             3181..4062
                     /locus_tag="PKK76_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNL69186.1"
                     /translation="MGRVEVLKTYKLYIGGQFPRTESGRYYPLLDAKGRTIANVSLSS
                     RKDFRNAVVAARAAQGSWAGKTAFNRSQILYRIAEMMEGRFEQFVSEIVLQGKSRKAA
                     EEETRTAIDRVVYYAGWCDKFQALFSTVNPVASSHFNFSVPEPTGVVAIIADPKTSLI
                     GIVSAIMPVIAGGNTCVALASESLPLCAVTLGEVLETSDLPAGIVNILTGSASELAAH
                     FASHMDVNAVVDCSGSVERKKLIQEAAALNIKRAFFYEKDWIKPDAESPYLILDLQEI
                     KTTWHPIENIGVGGAKY"
     gene            4133..>4814
                     /locus_tag="PKK76_11715"
     CDS             4133..>4814
                     /locus_tag="PKK76_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69187.1"
                     /translation="MQGAVRNAFAALFFSLLVASCGPRHKENPLLSLLLGTTPWAVPV
                     FSYAGPGNQGTNMENLLVATIGTATSSIQCGFQDVNLGSVMNALQFAKSRGIVVRVGI
                     DEDNRNMPGYRRLSGFLSTQGDDHEFWLGNSGSGQAYTNICVADKSRVFISTAPPTMQ
                     GFYSEPAFAMYFQDEETELPRKFQSEVDLLTHGSFGSNKQVLNRRNYYLLSDMEVGVY
                     FAPKESAFE"
BASE COUNT         1199 a         1119 c         1329 g         1167 t
ORIGIN      
        1 cctgcgcgat actccagcag ctctgggtct tccaccggtt catgaatacg caggagaatc
       61 agatccattc aaagaagcac agacacagag cggccgcgaa atattcataa actatatatt
      121 aaaaaatcgt atattatgga ttgtatgcat cgctaatata ttcgtctata ttgtgcgaat
      181 cggcgtcttt gactgggcac ccaagtttct ggtgagtgaa cgcggtttta ctacgaaaga
      241 ggccagcgtg gcgggctttg cttttgaggt ttctgggatc tttggagcga tcgcggctgg
      301 ctggttgtca gacactgcct ttccgggccg cagaggtgcg atcgccactg tatttatggt
      361 gtggcttgcg gccgccatag gaatcgtttt tgcttctcct tccggtaatt tctggctgat
      421 gatttttgct atggtttctg taggattctt tgtctacggg ccgcaggttc tggtggcggt
      481 ggtcgcggcg gagaatgtgg atgcgcgcgc agtcggcaca gcagttggaa tgacagggtt
      541 ctttggctac gtgggagcaa ccatttgcgg agtcgggact ggttatgtcg tagatcactt
      601 cggttgggat gtcgccctgg tcgtctattt gatatcggct ctcattggtg cgggcctctt
      661 cgcgatcgcc tggaagcccg ttatgcgaag ataggcactc ttcgcgcgct ttttgattga
      721 ctggttttcc tttcgagttc attttctgca cagagggcgc acagccttcg tacatcgaat
      781 gaaatcagca gctgttgttc cagactttcg ctacactccc gtgattgacc aggttggagt
      841 agaagagcgc gtggcgcgtt tcagttcgcg cagtattaag aaggagtcca aggtccaggg
      901 gctcaagctt gcactgagca tgatagacct caccacactc gaaggcaagg acacagaagg
      961 gcgagtgcgt cagatgtgtt acaaggcagc gcacctccat gatgttcttc ctggattgcc
     1021 aacggttgcc gcagtgtgcg tgtatccaac tcatgtgaag actgcgaaag acgcactgaa
     1081 aggaacagga atcaaagtag cctctgtcgc cactgcattt ccaagcggca atgccactct
     1141 tgatgtgaag attgcggata ctaagttcgc cgtggaaagc ggagcggacg aaatcgatat
     1201 ggtaattgcg cgaggggaat ttttgcgagg caactattcg ttcgtgtttg atgaaatcgc
     1261 ggcagtgaaa gaagcgtgcg gcaaagctag attgaaagtg attctggaaa caggagaact
     1321 gtccacactg gataacgtca ggcgagcaag tgacattgcc atttacgcgg gagcggattt
     1381 tatcaaaact tctaccggaa aaattcaacc cgcagccacg atgcccgtga ctcttgtcat
     1441 gctcgatgca atcaaggatt actattacca gacaggaacc atgatcggga tgaagcccgc
     1501 gggaggaatt tcaaccgcga aactcgccct gcaatatctg gtgatgctct atgaaaccct
     1561 gggaagcaag tggatgacca atcaatggtt tcgctttggc gcatccagtc ttgcaaacga
     1621 catcctcatg cagcttgcga aggaagcgca cggggtgtac caatcaccgg actatttttc
     1681 gaaggattag gagaccgcat ggcaacgcag aagaaagagg gcccgcgtct ggattttttc
     1741 tcggactgga aatattcgcc ggctccagaa tcaaaagaac acattcgact gaacaaaaag
     1801 tatgatttgt ttatcaacgg caaatggcag aagcccaccg gatcaaagta tttcgccacg
     1861 cacaatccgg ccacagaaga gaagctcgct gaagtagcgg acgcggggga aaaggacgta
     1921 gatgcagcgg tccaggcagc cagatccgcg tatgagaaaa cgtggagaaa gatgcccggt
     1981 cgcgaacgcg gtaaatacat ttatcgcatc gcacgaatga tccaggaaag agcgcgcgaa
     2041 ctctccgtga ttgaaaccat ggacggcgga aagtccattc gagaatcgcg cgacattgat
     2101 gtgccacttg cagccagtca tttcttctac tacgccggat gggcggataa gctggactac
     2161 gcttttccaa atcgcaaacc gactccgctc ggagtggccg gccagatcat tccgtggaat
     2221 tttccattgc tcatggcggc gtggaaaatc gcaccagctc tagcatgcgg aaatacagtc
     2281 gttttgaaac cggcggaaac aactcctctc actgcgctga aattggctga aatcattcag
     2341 gatagcggcc ttccagacgg agtggtcaac atcattacag gcgccgggaa aaccggggcc
     2401 gccattgtga atcatcctca tgtaaacaag gtggccttca ccggcagtac ggacgtggga
     2461 agaatcatcc agaaagccgt cgccgggact gcaaagcgcg taactctcga gttaggtggt
     2521 aaagctgcca atatcatctt tgaagatgca gccattgacc aggcagtcga aggcattgtg
     2581 aatggaatct atttcaatca gggtcatgtg tgctgtgctg gatcgcgtct ctttgtccag
     2641 gaaggagtcg cggatcttgt cattcgcaaa ctgaaagatc gcatggaaac gctcatcgtg
     2701 ggggatcctc tcgacaaaaa tacggatgtg ggcgccatca actccaagca acagctccag
     2761 gtaatcacca actacattca aattggaaag gaaggcggag ctgagtttta tcagagttca
     2821 tgcgaacttc caaagaaagg atactttgtt cgtcccactc tctttctcaa tgtatcccag
     2881 tctcatcgca ttgttcagga agagatcttc gggccagtac tggccgtgca gacctttcgc
     2941 acgattgaag aagtgattga aaaggcaaat aacattccct acgggctctc tgccggagta
     3001 tggacggaca agggttcaaa gatcttcaat cttactggaa agcttcgcgc aggagtgatc
     3061 tgggccaaca cttacaacaa gtttgatccc acatcgcctt ttggcggtta caaggagagc
     3121 ggctttggtc gggaaggggg cgtgcatggt ctccttcctt atctgagtct gtgaggaagt
     3181 atgggtagag tagaagtact taagacatat aaactatata ttggtggtca gtttccgcgc
     3241 actgaatcgg gaagatatta cccgcttctg gatgcgaaag gtagaacgat tgcgaatgta
     3301 agtctctctt cgcgaaaaga ttttcgcaat gccgttgtgg ccgcgcgagc agcccagggt
     3361 tcatgggcag gaaagactgc attcaatcga tctcaaattc tatatcgcat tgcagagatg
     3421 atggaaggtc gtttcgaaca gttcgtgtct gaaatcgtat tgcagggaaa atccaggaaa
     3481 gcggcagaag aagagactcg cactgcaatc gatcgtgtag tttactacgc aggctggtgc
     3541 gataagttcc aggctctctt tagtacggtc aatccggttg cttcatcgca cttcaatttc
     3601 tcggtgccgg agccaacggg agtcgtcgca atcatcgcag atccaaaaac atctttgatt
     3661 ggaatcgttt ccgcgatcat gcctgtgatc gcagggggaa acacttgcgt tgcccttgcg
     3721 tctgaatctt tgccgttatg cgccgttaca ctgggagaag tgctcgaaac atctgatttg
     3781 ccagccggga ttgtgaatat tttgaccgga tctgcctcag agcttgcggc gcatttcgct
     3841 tcgcacatgg atgtcaatgc agttgtcgat tgcagtgggt cggtggaaag gaagaagttg
     3901 attcaggaag cagccgcttt gaacatcaag cgtgcctttt tctacgaaaa ggattggatc
     3961 aagccggatg cggaaagccc gtatttgatt ctggatctcc aggagatcaa gacgacctgg
     4021 catccgatcg agaatattgg agtgggtggc gcgaagtact gaactgacgt tcaattgtaa
     4081 gcgttttgta gtctttcgaa gacagagtgc ttcgaggcag aaaggagggg ggatgcaagg
     4141 tgcagtgcga aatgctttcg cggcgttatt cttttccctt ctggtcgcgt cttgcggtcc
     4201 caggcataag gagaatcctc ttctcagtct gcttctggga acgactccct gggccgttcc
     4261 agtattttcg tatgctggcc caggaaatca gggaacgaac atggagaact tgcttgtggc
     4321 aacgatcgga acggccacat cgagcattca gtgcgggttc caggatgtga atctgggaag
     4381 cgtaatgaac gcacttcagt ttgcgaaatc acgcggcatt gtggtgcgag tgggaataga
     4441 tgaagacaat cgaaacatgc ctggatatcg ccggctttct ggatttctaa gtacccaggg
     4501 agacgatcac gaattctggc ttggaaattc tggttctggc caggcataca cgaacatctg
     4561 cgttgcagat aagtcgcgcg tgttcatctc tacagcgccg cccaccatgc aaggcttcta
     4621 tagcgagccc gcatttgcga tgtattttca ggatgaagag actgagcttc cgcgcaagtt
     4681 ccagtccgag gtggatctac tcacgcatgg atcctttggc tccaacaagc aagtcctcaa
     4741 tcgtcgcaac tactacctgt taagcgacat ggaagtggga gtctatttcg ctcccaaaga
     4801 gagtgcattc gaga
//
LOCUS       DAORFF010000282         4792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10132_length_4792_cov_3.392020, whole genome shotgun sequence.
ACCESSION   DAORFF010000282 DAORFF010000000
VERSION     DAORFF010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10132_length_4792_cov_3.392020"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..1049
                     /locus_tag="PKK76_11720"
     CDS             258..1049
                     /locus_tag="PKK76_11720"
                     /inference="COORDINATES: protein motif:HMM:NF025107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNL69188.1"
                     /translation="MKPDNLPFTKLVVALFDSGLWARMSAAARTLYPVLLRFSDGKFK
                     PVFPGTALLLKLTGFKQKHSIRDARNELVELGLIAITKGSGQKNTYYHFRFEWAGGPP
                     VTQGGSPGYPTEGLEAASPGGTELFSRGAGDGSPHNQIHITITNHLENRLPGELEAMI
                     KKFGEDKVRGAVTELTLAGLPANAANVEGVIQHANHAWTSLREKLTGSLSRESIRLLD
                     QAWMGETNGKLCFRDDLPGHLKKIVTRHGSGVEFVQSAVQTAATR"
     gene            1046..3586
                     /locus_tag="PKK76_11725"
     CDS             1046..3586
                     /locus_tag="PKK76_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNL69189.1"
                     /translation="MSSLLKISHPETLLFAQWQAPGSTDESGGRILSWQGPVMESPGL
                     LACVGTFDTIRSFNSIKLKFHSHLKEFLPNTFRFEISNDGVVWEPILHEANFHGSWMD
                     EAIWHFSLISARMIKFLFLVDRQDSSGHYLAAFGEFAVMVSGIVQVDVSSELDRLWVK
                     DNLVDQRKDYGWSSALRLKKQPEFIHLDLGSINRVGEIRLLSKNDRETFFPEAFRLSY
                     SEDNITYHHLIEETGFMAEPGTWYKWRFLPTNIRFLRIDITEGAKTREGKYVSQIIEV
                     ELYAVPDLVDKTEKSLPEPIPYASVLRSGIMRFAVDGEVKEGVAVQASDRRLRDSTTE
                     ARGIVELASDGEDSPGVAVQGSDRRLKYATEDLPGIVRLARDGETRTGHAVQGSDRRL
                     RLASEEEAGLVELAADGENRPGVVVQGSDSRLRYATEKAHGIVKLAAIGASNPGESVQ
                     ANDPRLRDATEEAKGIVRLARSGEAAGDAAVQGDDPRLRAASTENLGIVELARDGEVK
                     EGAVVQSHDKRLHPATESSPGIMEFCAPGNSIAGRAVQGHDPRLSDARDPKPHNHDYA
                     PRVHNHSDHEGSLHIRVEGGTAIKKIVSPPPSNAPVTGQSTGAGSGILGIGERDGVAG
                     AGKSAGVLGLGLTQGAGVIGAAQNGSGGWFVSERGYGLVAGGMAASHDIHSSPYALLS
                     RGLSGFLDSMVVANPELEACIAVYFPVESGDVISPGDLVSSGKPGHVKKCKTAQDTAV
                     VGVVVNRAAVVLGAPGNYLPDPAKPDGFSDPQTPKGHVLVAVSGIVSIRTVAQSGIRP
                     GDLLVASSQAGKAEKWQSDKAKPGSLVARSLEELSKGEGLLRCLLVSG"
     gene            3583..4443
                     /gene="metF"
                     /locus_tag="PKK76_11730"
     CDS             3583..4443
                     /gene="metF"
                     /locus_tag="PKK76_11730"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNL69190.1"
                     /translation="MKMITDMLKEKRPLYSFEFFPPKTSEGETKLFSTVQELSALKPD
                     FVSVTYGAGGSTRDKTRSWVETIQNRYGIPAMAHFTCVGSSRAEIRAYLEDLSRSGIR
                     NIMALRGDPPKGETSFKPAPDGLAHGSEMIAFIRQTGLPFALGGAAYPEVHPEAISPE
                     DDLVRLREKVKCGADFLVTQLFFDNSKYFSFIKKADVAVPVVPGIMPITAFQQVERFT
                     KMAGCSIPQSLLRAIADCGEDTKKLLEVSLAFTEQQCRELLDAGAPGIHFYTLNQSHA
                     TMEILERIRR"
     gene            4446..>4792
                     /locus_tag="PKK76_11735"
     CDS             4446..>4792
                     /locus_tag="PKK76_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNL69191.1"
                     /translation="MSEKRTLTLAAINDITTRINSEQDLPGLLTVIMETARQLLNTEA
                     ASLLLYDPEREELIFDIVRGPRGGMLANKRIPKGRGIAGLCAEKKEPIIVDEAQTDPR
                     VMRSFDAESNFTTR"
BASE COUNT         1195 a         1144 c         1333 g         1120 t
ORIGIN      
        1 acaagcgcaa gaatccaatt agtatgtaat acatactaat tgcctggatt tttttaagaa
       61 aatgatttga cgtacccgta atgtgacata atattttgga tgtctctttt cgtcaggaat
      121 gagacacgcg aaagcgtaaa aaaaagccct ccgctggaac ggagggccgg ggtttcaaga
      181 ttatgtcaca taaatacctg ctgtaccacg atttgtcaac aaatttggct tctctacact
      241 gaaattagag aatccgcatg aaaccagaca atctgccttt caccaagctg gtggtggctc
      301 tgtttgactc cggcctgtgg gccaggatgt ctgcggcagc cagaacgctc tatcccgtct
      361 tactccgttt ttcggacggc aagttcaaac ccgtttttcc tgggacagcc ttgctgttaa
      421 aactgaccgg gtttaagcag aagcacagca tccgcgacgc caggaacgaa ctcgttgagc
      481 tagggctcat cgccatcaca aagggatccg gccagaagaa cacgtactat catttcagat
      541 ttgaatgggc ggggggtccc ccggtcaccc aggggggatc tcccggctac cccaccgagg
      601 ggctggaggc cgcctctccg gggggtaccg aactgttctc gagaggggca ggggacggtt
      661 ccccgcataa ccagattcat ataaccataa ccaatcatct agagaatagg cttcccgggg
      721 aactcgaagc aatgattaaa aaattcggtg aagacaaagt gcgtggagcc gtcactgagc
      781 taactctggc cggtttgcct gccaacgcgg cgaacgtaga aggtgtcatc cagcacgcaa
      841 atcacgcatg gacatctctc agagaaaaac ttacgggctc cctgtctcga gaaagcattc
      901 gcttgctaga ccaggcgtgg atgggagaaa cgaatggaaa gctgtgcttt cgtgatgacc
      961 tccccgggca tctgaaaaaa attgtaactc gccatggaag tggcgtagaa tttgttcaat
     1021 ctgcagttca gacggcagcc accagatgag ttcactccta aagatttcac atcccgaaac
     1081 attacttttt gcccaatggc aagcacccgg cagtaccgat gagtcgggtg gtcgcatcct
     1141 gtcctggcaa ggccccgtca tggagagccc gggactgctt gcctgcgtcg ggacttttga
     1201 tacaattcgt tcttttaatt caatcaagct gaagttccat tctcatctaa aggaatttct
     1261 tccaaacaca tttagatttg aaatctccaa tgatggagtc gtatgggaac ccatcctgca
     1321 cgaagcgaat tttcatggca gctggatgga tgaagcaatc tggcactttt ccctcatatc
     1381 cgctcgcatg attaagtttc tgttcctcgt ggacaggcag gattcatccg gacactatct
     1441 ggctgccttt ggagaatttg cggtcatggt ttcgggaatt gtccaggtgg atgtgtccag
     1501 tgaactggat cgactgtggg tgaaagacaa tctcgtagac cagagaaagg actacggctg
     1561 gtcttccgct cttcgcctga agaagcaacc tgaatttatt catctggatc ttggatccat
     1621 caatcgtgtt ggagaaatac gactcttatc aaagaacgat cgcgaaacat tttttcctga
     1681 agcattcagg ctatcttaca gcgaagacaa tattacgtat catcatctta tagaagaaac
     1741 cggattcatg gcggagcccg gcacctggta taaatggaga ttccttccga cgaacattcg
     1801 tttcttgcgc attgacatta cggaaggtgc aaagacgcgc gaagggaagt acgtatctca
     1861 aatcatcgaa gtggaattgt atgctgttcc ggatcttgta gacaagacag aaaagagtct
     1921 tcctgaaccc atcccttatg cttctgtttt acgaagcgga attatgcgct tcgccgtgga
     1981 cggagaagta aaggaagggg tggctgttca ggcaagcgat agacgacttc gcgattccac
     2041 cacagaggcc cgcggcattg tagagcttgc ctcagacggg gaagactctc cgggagtggc
     2101 tgttcagggt agcgatcgaa ggttgaaata tgcaacggaa gatcttcctg gaattgtgag
     2161 actggcgcgc gacggagaaa cacgtacggg tcacgcggtt cagggaagcg atcgcaggct
     2221 tcgcctggcg agtgaagaag aagcaggtct tgtagagctc gcagccgatg gggaaaacag
     2281 acctggagtt gtggtgcaag gcagcgatag tcgcctcaga tatgcaacag agaaggctca
     2341 cggcattgtc aagcttgcag ccattggtgc gagcaatcct ggcgaaagcg ttcaggcgaa
     2401 tgatcccagg ttgcgcgatg caacggaaga agcgaagggg atcgttcgtc tggcacgaag
     2461 cggtgaggcc gccggagacg ccgccgttca aggggatgat ccgcgccttc gtgcagcgtc
     2521 cacagagaat ctcgggattg tggagctggc ccgtgatgga gaagtgaagg aaggggccgt
     2581 cgttcagagt catgacaaac gacttcatcc ggctacagaa tcatcccctg gaattatgga
     2641 attttgtgcc ccgggcaatt caatcgccgg gcgcgcagtg caaggacacg atcctcgtct
     2701 ttcagatgca cgggatccaa agccgcataa ccacgattat gcgccgcgag ttcacaatca
     2761 ctcagatcat gaaggaagtt tgcatattcg cgttgaaggg gggactgcga tcaagaagat
     2821 cgtttctccg cctccttcga atgcacctgt aacagggcag agtacgggag caggttctgg
     2881 gatactcgga atcggagaaa gagacggagt ggcgggcgcc ggaaaatcgg caggtgtgct
     2941 cgggcttggt ttaactcagg gcgccggtgt gattggcgca gcgcagaacg ggtcaggtgg
     3001 ctggtttgta tccgagagag gctatggact tgtggcagga ggaatggcag cttcgcatga
     3061 tattcattcc tctccttatg cactattgtc tcggggtctg tctggctttc tcgactcgat
     3121 ggtggtggcc aatcccgaac tggaagcatg cattgcagtg tatttcccgg ttgaatcggg
     3181 cgatgtaatc agtccaggag atcttgtttc ctctggaaag cctggtcacg tgaagaaatg
     3241 caagactgct caggatacgg ctgtggttgg tgtagtcgtg aatcgcgcag cagtcgtgct
     3301 gggtgctccc ggaaattatc tccctgatcc agcgaagccg gatggtttca gcgatcccca
     3361 gacacccaaa ggccacgtgc ttgttgcagt ttctggcatt gtatcgattc gaaccgttgc
     3421 acagtccgga attcggcctg gcgatctgct cgttgcgtct tcgcaagcgg gcaaagctga
     3481 aaaatggcaa agcgacaaag caaaaccagg tagcctcgtg gcacggagcc tggaagaact
     3541 ttcaaaaggg gaagggcttc tgcgctgcct gctcgtatcc ggatgaagat gattacagac
     3601 atgctcaagg agaagcggcc gctttactct tttgaattct tcccgcctaa aacttcggaa
     3661 ggggaaacaa agcttttctc aactgtccag gaactgtctg ctttaaagcc tgattttgtt
     3721 tctgttacct atggtgcggg tggttccact cgagacaaaa cgcgcagctg ggtagaaacg
     3781 attcaaaatc gctatggaat cccggcgatg gctcatttta catgcgtggg ctcttcgcgt
     3841 gctgagatcc gggcttacct ggaggacctg agccgcagcg ggattagaaa catcatggcg
     3901 ctcagaggcg atcctccgaa aggggagact tccttcaagc cggctccgga tggacttgct
     3961 cacggaagcg agatgattgc ttttattcgt cagaccggtt tgccttttgc gctcggtgga
     4021 gccgcatacc cggaggtgca cccagaagca atatctccgg aagacgacct ggttcgcttg
     4081 cgcgaaaaag tcaagtgcgg tgccgatttt ttagtgacac agctattctt tgacaattct
     4141 aaatacttta gttttattaa gaaagcagat gtggcggttc ccgtggttcc ggggatcatg
     4201 cccataactg cgttccagca ggtggagcga tttacgaaga tggcaggttg tagtattccg
     4261 cagtctcttc tccgtgcgat tgctgattgc ggggaagaca caaagaaatt gcttgaggta
     4321 agcctggctt tcacagagca gcaatgcaga gaacttctgg atgcgggcgc tcctgggatt
     4381 cacttctata ctctcaacca atctcatgca accatggaga tcctggagag aatccggcgt
     4441 taactatgtc cgagaaaaga acccttacac tggccgcgat caacgatatc acgacgcgga
     4501 tcaactcgga gcaagatctg cccgggttgc ttacggtgat tatggaaacc gctcggcagc
     4561 ttttgaatac ggaagccgca tcgcttttgc tctacgatcc tgagcgcgaa gagttgatct
     4621 ttgacattgt aagaggtccc cgtggaggca tgcttgccaa taagagaatt ccaaagggaa
     4681 ggggaatcgc gggcctctgt gctgagaaaa aagaaccaat cattgtagat gaggcgcaaa
     4741 cggatccgag agtaatgcgc agctttgatg cagaatctaa tttcaccacg cg
//
LOCUS       DAORFF010000283         4792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10133_length_4792_cov_3.321089, whole genome shotgun sequence.
ACCESSION   DAORFF010000283 DAORFF010000000
VERSION     DAORFF010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10133_length_4792_cov_3.321089"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..463)
                     /locus_tag="PKK76_11740"
     CDS             complement(62..463)
                     /locus_tag="PKK76_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNL69192.1"
                     /translation="MIVQDYMTKDPVTVRADDPLSRVGEVFDVERFRHLPVVDASRNL
                     VGILSDRDLRNIQCAMEILETSVQGEQHVLVKDVMTATVRSVSAGDSLKSAAELFLSL
                     RVGALPVVEDGKLAGILSYTDVLRAFISLAK"
     gene            complement(510..2378)
                     /locus_tag="PKK76_11745"
     CDS             complement(510..2378)
                     /locus_tag="PKK76_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69193.1"
                     /translation="MARLLICTLLLISSSVLADTILLKNGQKIEGLIVYQDADVVHLQ
                     TADKTIVLQKNLVKRISYSPLQKPDAKKQDNEAEIAKEEKEKEQKEKAKKAEAERQRL
                     QQIQKEKEKQELARKERLKQEQAKRERERAMREKKEEEDRKLAEQKLAEEKKAADEKL
                     EAEKRETEKREAEKRAEEKKQADKLEAARLEEERKKQEEAERLARIEAEKKQKEAAEQ
                     ARLAKIEEEVRQKKEREAAEQKRIEDAKIAAEQKIKNPNYLLFGIAIGKGASKTSAGA
                     NALYNIESYSRFTGGYGLRTRYSASTSSDSSSSSSSSSSSSGLRSISGIHGASGFTSD
                     QTSASEANASNPFQAGSHKGGSLHLLYGIGMWRFVFDVERFKNKRIDRLEYGAGIAGP
                     PGASLGVVYGAFTGTTEQFNEVKIGTEYSLVRKDFLDVFVLGGGQRRAHNVAGETIQN
                     SILPGQSTVPTLLSGEKASTLMRGLYAGGGLELGQSWGLRTSIEVYSMRGNLSAKAIG
                     LGRFFTFTPGTGQIALPSVDQRGTESGLVLTAGLFIRILEKYYFRLDYSGDSGTFKES
                     KTTLFGLDYSSDPAAVLAGVILPGLYTSNPMLARSPAKVRYSSLTAGFSMRIDFAR"
     gene            2490..3254
                     /locus_tag="PKK76_11750"
     CDS             2490..3254
                     /locus_tag="PKK76_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNL69194.1"
                     /translation="MLDLEIKDGIARITMNRAPLNALNPELISQMRKTLADLAKNREC
                     RALLIDSAIDSFFSNGLDPEAMLALDVPGRADVFRSFLSLARDLYAFPAPTLSAIAGH
                     AMAGGAVIGIATDFRFLAEGKYSYCFSEVKVGLTIPEFLLEMISQVTGPHHLRSLAML
                     AERFRPEECLAMGLADKIVPRENLLIESRKYLLSLSDRPLSSMRSVKENMHAGTLARI
                     DRFLESQEDTFFQFLGANFEEGLKAVLERRRPQFTD"
     gene            complement(3261..3677)
                     /locus_tag="PKK76_11755"
     CDS             complement(3261..3677)
                     /locus_tag="PKK76_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69195.1"
                     /translation="MTFVEFTMSEGSMLVNPFALRAATPVTDGQGRTLHYMLEFVDGS
                     NLRIDNQRDMDLIHKYSAMFTHTRTAGKTVMTSSGSFHSVNVNIIQNYESVESKSGQI
                     RLVIITLNGGEKRIFDNDYDINVLQEWIRAQNVIVK"
     gene            3756..>4792
                     /locus_tag="PKK76_11760"
     CDS             3756..>4792
                     /locus_tag="PKK76_11760"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNL69196.1"
                     /translation="MIQAAVGYSDDVDSADAMREAIAQCRAQMQAPPKAAFLFASYEQ
                     DHDALLAEFNRAFPGLPLIGGTSTAQITSSGRYAEDSVALILFASDDVEFGIGLGRNL
                     SVDPLRAAREAVQQAKSNLSAPPTLAIAFPESLTTSGERIIEGLKAELGTAFPIFGGT
                     TSDSHTGKGTFQYFRTERLTDSLPVLLLSGNVRFSFAVRSGYKPIGKKRTATKVKGNR
                     VYELDGESLLDLYQKYLGDINLVSPEYPLAVFENGSDQFYLRTSQFFDAKDGCVIFDG
                     DIPEGAVMQFTDGTRSDLLDAAGSAVRSAIQAFPGQPRVALIFSCATRVLTLGTKVKE
                     EYAVLDSVRPGG"
BASE COUNT          998 a         1228 c         1185 g         1381 t
ORIGIN      
        1 ggcgaagacg aggctggtga actttacgtg gtcgactatc gctctggatc gattctcaaa
       61 atcatttcgc aagagaaatg aacgcgcgaa gtacatccgt gtagcttaga attcccgcca
      121 gttttccgtc ctctaccact ggaagcgccc ccacgcgaag agataggaat aattcggcgg
      181 cagatttcag agaatcaccc gcagaaacgc ttcgcacagt cgcagtcatc acatctttca
      241 cgagaacgtg ctgctctcct tgaacgctcg tttcaaggat ttccatggcg cactgtatgt
      301 tgcgcaaatc acgatctgat agaattccca ccagattgcg cgacgcgtcc actacaggga
      361 gatgcctgaa cctttccacg tcaaaaacct ctccgactct ggagagggga tcatctgctc
      421 tcactgtgac ggggtctttc gtcatgtagt cctggacaat cataatgctt gaatatgcgc
      481 ggctttctct ctgtcaagct atgggcgcgt catcgcgcaa aatcaattcg catagaaaaa
      541 cccgcggtta gactgctgta tcgcactttc gcaggggagc gggccagcat tggattgctc
      601 gtatacagac cgggcaggat gactcctgcg agaacggctg cgggatcact ggagtagtcc
      661 aggccaaaca aagtcgtctt tgactccttg aatgttccgg aatctccgga atagtccagg
      721 cgaaagtaat atttttcaag tattcgtatg aacaacccgg cggtcaaaac aagacctgat
      781 tccgtacccc tctgatccac gctcggaaga gcgatctggc cggtgccagg agtgaatgtg
      841 aaaaatcgac ccagcccgat tgctttcgcg ctgagattcc ctctcatact gtacacttct
      901 atagacgtgc gcaatcccca cgattgaccg agctcgagtc ctcccccggc atacaatcca
      961 cgcatgagtg tggacgcttt ttctcccgag aggagagttg ggaccgtcga ctgaccggga
     1021 agaatgctgt tctggatggt ttctccggca acgttatgcg ctcttctctg accacctccc
     1081 agcacaaaca cgtccaggaa atcctttcgc acaaggctat actcagtgcc aatttttacc
     1141 tcgttgaatt gctccgtggt gcctgtaaat gcaccgtaga ctacacccag gcttgctcca
     1201 ggtggcccgg cgattccagc cccgtactca agccgatcta tgcgcttgtt cttgaatcgt
     1261 tctacatcga atacaaagcg ccacatgcct attccgtaaa gcaggtgcag cgagccgcct
     1321 ttgtgagatc cggcctgaaa tgggttggat gcgtttgcct cgcttgcgct ggtttgatcg
     1381 cttgtgaaac cggatgctcc gtggatcccg gaaatggaac gtagtcctga acttgaagag
     1441 ctggatgaac tggaagaact tgagtcagat gaagtggatg ccgaatatct ggttctcaaa
     1501 ccatatccgc ctgtaaaacg ggaataggat tcgatattgt agagtgcgtt tgcacctgca
     1561 cttgttttcg aagctccttt tcctatggcg attccgaaaa gaagatagtt aggattcttt
     1621 attttctgct ctgcggcgat tttcgcgtct tcgattcgtt tttgttcagc cgcctctctt
     1681 tctttcttct ggcgaacctc ttcttcaatt ttggccagac gtgcctgctc tgctgcttcc
     1741 ttttgctttt tctccgcctc tattctggcg agtcgttccg cttcttcctg tttctttctt
     1801 tcttcttcga gtcttgccgc ttcgagctta tccgcttgtt tcttctcttc cgcgcgtttt
     1861 tctgcttccc tcttttctgt ttcgcgcttt tccgcttcca gtttttcgtc tgccgctttt
     1921 ttctcttccg ctagtttctg ttccgccagt ttcctatcct cttcctcctt tttctctcgc
     1981 atcgcgcgct cccgctctct ctttgcctgt tcctgtttga gtctctcttt gcgcgccagt
     2041 tcctgtttct ctttttcttt ctggatttgc tggagtcttt gtctttccgc ttctgccttt
     2101 tttgctttct ctttttgctc tttttccttt tcctcttttg cgatttcagc ttcgttgtct
     2161 tgcttctttg cgtctggctt ctgcagcgga gaatagctga ttcgtttcac cagattcttt
     2221 tgtagaacga ttgttttgtc tgcagtttgc aggtggacta catcggcatc ttgataaaca
     2281 atcaagccct cgatcttctg gccatttttc agtagaattg tatccgcaag aaccgacgag
     2341 gaaatcagga gcagtgtgca aatcagcaag cgtgccatag gaagcttctt gtggatggtc
     2401 cctgtatgtg caatcagaat gcacggacgg acctatgact tttcttatga ttgggcttgc
     2461 ctttgtgcgg gttggttcct cagtcgggca tgctggattt agaaatcaaa gacggaattg
     2521 cacgcatcac catgaaccgg gcgcctctca atgcgctgaa tcccgaattg atttcgcaga
     2581 tgcgaaaaac cctggcggac ctcgcgaaga atcgggaatg cagagcactc ctgattgatt
     2641 ctgcaattga ttcattcttt tcaaacgggt tggatccaga agcaatgctt gccctggacg
     2701 ttcccggtcg cgcggacgtt tttcgaagtt ttctttctct ggcccgggat ctttatgcct
     2761 tcccggctcc tactctttct gccattgcgg gtcatgcaat ggcgggtggc gctgtgattg
     2821 gaattgcaac ggactttcgc tttctggcgg aaggcaaata ttcttactgt ttctctgaag
     2881 tgaaagttgg tctgacgatt ccggagtttc tcctggagat gatttcgcag gtaacgggcc
     2941 cgcatcattt gcgatctctg gccatgcttg cagaacgctt tcgtcctgag gagtgtctgg
     3001 ccatgggcct tgcggataag attgtgccca gggaaaacct cctcattgag tccagaaaat
     3061 accttttgtc cctttcagat cgccctcttt ccagcatgcg gtccgtgaaa gagaacatgc
     3121 acgcgggcac tctggccagg atcgacaggt ttctagaaag tcaggaggac actttctttc
     3181 agttccttgg agcgaatttt gaggaaggat tgaaagcggt actggagaga aggcgtccgc
     3241 agtttacgga ttagttgaga ctatttaaca attacgtttt gtgcgcggat ccattcctga
     3301 agcacattga tgtcatagtc gttgtcaaaa atgcgctttt ctccgccgtt gagtgttatg
     3361 atcacgaggc gaatttgtcc gctctttgat tccacggatt cgtaattctg gatgatgttc
     3421 acattcacgg agtgaaacga tccggaggag gtcatgaccg tttttccggc tgttctggtg
     3481 tgcgtgaaca tggcgctgta cttgtgaatc aggtccatat ctcgctggtt gtctatgcga
     3541 aggttgcttc catccacaaa ttccagcata taatggagag ttctgccctg gccgtccgtg
     3601 actggagtgg ccgcgcgcaa tgcaaatggg ttcaccagca tggaaccttc gctcatggtg
     3661 aattcaacga atgtcatact tcagcgcccg cttttcctcc ggtttatgga atgagggctt
     3721 gacgggaatc aaaattttta gcaaccctgt tttccatgat tcaagctgct gtgggttaca
     3781 gcgacgacgt cgattctgcg gacgccatgc gcgaggcgat tgctcaatgc agggcccaga
     3841 tgcaggctcc tcctaaagca gcgtttttgt ttgcatctta tgagcaggat catgacgcgc
     3901 ttcttgcgga attcaatcgc gcctttccgg ggcttccact catcggaggc acgtccacag
     3961 cacagatcac ttcctccggc agatatgcgg aagattctgt tgcgttgatc ctttttgcat
     4021 cggacgatgt tgagttcgga atcggactgg ggcgaaactt atctgtagat ccacttcgtg
     4081 cagcgcggga agcagttcag caggcaaaat cgaatttgag cgctccacca actcttgcga
     4141 ttgcatttcc tgagagtcta acaacgagcg gtgagcgaat catagaagga ctcaaggcag
     4201 aactgggaac tgcgtttcca atttttggcg gaaccacttc tgattctcac actggaaaag
     4261 gaacctttca gtattttcgc acagagcgtc tgactgattc acttcccgtc cttcttctat
     4321 ctggcaatgt gcgattcagt ttcgctgtgc gaagcggcta caagccaatt ggaaaaaagc
     4381 gcacagccac gaaagtaaaa ggaaatcgcg tgtatgaact ggacggagaa tctctcctgg
     4441 atctctacca gaaatatctg ggagacatca atctggtgtc gcctgaatat cctctcgcgg
     4501 tgtttgaaaa cggcagcgat caattctatt tgcgcacttc tcaattcttc gatgcgaaag
     4561 acggctgcgt gatttttgat ggcgacattc cagaaggggc ggtgatgcag tttacggatg
     4621 gaactagaag cgatttgctg gatgccgcag gatccgccgt tcgctccgcc atacaagcgt
     4681 ttccgggtca accgcgcgtt gctctcattt tctcttgcgc tacacgggtt cttactctgg
     4741 gcacgaaggt caaagaggag tatgcagttc tggatagtgt tcgtccgggc gg
//
LOCUS       DAORFF010000284         4757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10245_length_4757_cov_3.940026, whole genome shotgun sequence.
ACCESSION   DAORFF010000284 DAORFF010000000
VERSION     DAORFF010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10245_length_4757_cov_3.940026"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PKK76_11765"
     CDS             complement(<1..319)
                     /locus_tag="PKK76_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69197.1"
                     /translation="MNNNYKMPRLIFAGLFFFGFVSAAHSQTLDPGTMDRLRTALGQV
                     RHPDQVDMLQMAQSIAPVNTDAYLIALASQPTSVPGLRLRAYGLLGHFSASPATKAFL
                     AQQV"
     gene            complement(327..596)
                     /locus_tag="PKK76_11770"
     CDS             complement(327..596)
                     /locus_tag="PKK76_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69198.1"
                     /translation="MAHEGGHWLGLFHPVESNYDTTQNNYNRDAISETPLCTAAGVGL
                     AACDFTAVNNAGSRNVMFWAGTAGFDQSDLTGEQGFVLRRHPLAY"
     gene            complement(551..1681)
                     /locus_tag="PKK76_11775"
     CDS             complement(551..1681)
                     /locus_tag="PKK76_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69199.1"
                     /translation="MRRSIVLAALLVLLTCTSCALGLLQLPGSKSDDDNLSLMLLGIL
                     ALQPAGPRYEFQETTVTPTTANANYYGAGITGYSVVVSGSTVGPFNPSEIFQAEQNHA
                     VSNAYIDFQQRVDSSGWFFSRNAPLSASAQNGSTFHLYGATPNLYTSIATTATASSTQ
                     TLNFAIIAPSNSLPMTYRRIRALIPASSEESNFSPDSTTVNYKLRGMSKVWSTEKRLN
                     VNLIFISGSNPAASTTGIAEAITRMTDLYTQNTVRIRPVISSTTIVLPSYLTLTSLST
                     DTTATGSLGGLMVNTASAQRADALNIIFVKNETAVGGVLGISTGIPGLPGRVGTRQSG
                     MVVMFDGHLASAGAVPSSAEQRLIGETMWPMRAVTGSGFFIQ"
     gene            1922..2479
                     /locus_tag="PKK76_11780"
     CDS             1922..2479
                     /locus_tag="PKK76_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69200.1"
                     /translation="MEKPVLLASATQSQCRVVLQQRVGYVLFHLPFNEITSGQIKSAL
                     QSQKEPYSIRYREMLTPADIGLNILGFLFSVLTVTKQVEACAAPEMVESSDQAKKARI
                     YSEFEKERKRFSRFEVPIGLTTTIEKKVRHRVYQKPEDGEAAMISREGSLTIKSNKLQ
                     LETEEGTVAIPEDSVLMIEVMIPEG"
     gene            complement(2480..3676)
                     /locus_tag="PKK76_11785"
     CDS             complement(2480..3676)
                     /locus_tag="PKK76_11785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNL69201.1"
                     /translation="MNSNPRIVLQPGREKSIKQRHPLVFSGAVAKWPTFEDGDLLNVQ
                     SHSGEYLGCGQFRKGASLAGRMLWFAESPEDTDFQQWAVDRFVAALGLRTSLPLDGNA
                     WRAVAGESDGMPGVTLDRYAEYWVIQISSVGMERNKEHLISAFRSASAKAGVKCKGMY
                     QKVVPRTSAEPGTLESSAWIYGSGVDSVTIQENGLSFRVEFEGSQKTGFFLDQRENRA
                     YLRTLSRNRRVLNCFSYTGGFSVYAASGGASRVVSVDSSEKAIALAKENFSLNQISQA
                     NHGFEASDVFEYLRSVEEEFDIIVLDPPAFARTKRDLPQAMRGYRDIHRLAFQVIEKG
                     GLVLTSSCSHPVDSDLMRKIIFQAALEARRDVRILQSHRQSPDHPLSIFYPEGDYLKG
                     FLLHID"
     gene            3724..4134
                     /locus_tag="PKK76_11790"
     CDS             3724..4134
                     /locus_tag="PKK76_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69202.1"
                     /translation="MPRLTRYFVKSAFICLVLSFMTGIILHLDFLIPNAGVLRPVFYH
                     LFLVGFTTQLIFGISYWMFPTLSRDNLRGNESLGWISYVALNGGLLLRATAEPAVALS
                     PVPALRITLAAAAFLQLIAVVAYVALIWKRTKAK"
     gene            4134..4559
                     /locus_tag="PKK76_11795"
     CDS             4134..4559
                     /locus_tag="PKK76_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69203.1"
                     /translation="MPAISRILVRTSFCYLILGSAIGTWLLLDKAASTPILEGVRNIH
                     REIMLAGWIVQLVIGTATWILPRNDRSRGDMKSVWIAYALFNAGILVAAIGTILGNQA
                     VLTAAHCCYAAGAISFAKHAWPRIKSLPQDQERPNARPP"
     gene            complement(4529..>4757)
                     /locus_tag="PKK76_11800"
     CDS             complement(4529..>4757)
                     /locus_tag="PKK76_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNL69204.1"
                     /translation="ALEKRADGSTLFTPLLAVSLMVFFALCCQCMSTVVTVQRELQSW
                     GWAGFMFAYMTGLAYLFALLIYQGGRAFGLS"
BASE COUNT         1234 a         1258 c         1175 g         1090 t
ORIGIN      
        1 taacttgctg cgcaaggaag gctttcgtgg ctggacttgc agagaaatgt ccgagcaacc
       61 cataagcgcg caaacgcaat ccgggaacgc tcgtgggctg actggcaagc gcgatcagat
      121 acgcatccgt atttacgggg gcgatggact gcgccatctg caacatatcc acctgatctg
      181 gatggcgaac ttgcccgagt gcggtcctca gtctgtccat tgtgccagga tcgagtgtct
      241 ggctgtgagc ggcagaaaca aatccaaaga aaaagagacc cgcgaagatc aagcggggca
      301 ttttgtaatt gttgttcatg ttcacctcaa tacgcaagcg gatgtctgcg aagcacaaaa
      361 ccctgctccc ccgtcagatc tgattggtca aatccagccg tgccggccca gaacataaca
      421 ttgcgcgatc ccgcattgtt cacagcggta aaatcgcaag cagcgagacc cacacccgcg
      481 gccgtacaca aaggtgtttc agaaatcgca tcgcgattgt agttattctg cgtagtgtca
      541 tagttgctct ctactggatg aaaaagcccg agccagtgac cgccctcatg ggccacatgg
      601 tttcgccgat caacctttgc tccgcagaac tcggtacggc gcccgcactt gccagatgcc
      661 cgtcgaacat aacaaccatt cccgactgcc tggttcccac tcttcctggt agcccgggaa
      721 ttccagttga tatgcccaga acaccgccaa ctgcagtttc gtttttcacg aatatgatat
      781 tcaaagcgtc tgcacgctga gccgatgcag tgtttaccat caagcctccg agactgccgg
      841 ttgcagttgt atccgtactc aaacttgtga gagtgagata ggacggtaac acaatcgtcg
      901 ttgaagagat gaccggccga atcctaactg tgttttgagt gtatagatcc gtcatccgag
      961 taatcgcttc cgcgatcccg gtcgtggacg cggcaggatt ggaaccagag atgaaaatca
     1021 aatttacatt gagacgcttc tctgtggacc agactttgct cattccgcga agcttgtaat
     1081 tgactgttgt agaatccggt gagaaattac tttcttccga cgaagctgga atcagagcgc
     1141 gaatccggcg atatgtcatg ggaagcgaat tgcttggagc aatgattgcg aaattcaagg
     1201 tctgcgtact ggaagccgtc gcggttgtgg caatagaagt ataaaggttc ggtgtggctc
     1261 cgtagagatg aaatgtactt ccgttttgcg cagaggcaga cagaggcgcg tttcgggaga
     1321 aaaaccatcc gctgctatct acacgctgct gaaagtcaat gtaagcattg gacactgcat
     1381 gattctgttc cgcctgaaaa atctcagatg gattgaatgg gccgactgtg gatccagaca
     1441 caaccacgga atacccggta atccccgcac catagtagtt tgcattcgcc gtagtcggcg
     1501 tcaccgttgt ttcctggaat tcgtatctgg gcccggcagg ctgcagagcc agaatcccca
     1561 gcagcatcag actgagattg tcatcatctg atttggaacc aggtaattgt agcaaaccaa
     1621 gagcacagga tgtgcaggtg agcaatacaa gcagggcggc cagcacaatc gatcgacgca
     1681 tgcctcatgc tgaaggacag cccccgacca gtcaacacac aatggaacca ctcgctaaaa
     1741 aaccataaac tttctaaaat gaatcttgac tccgtcccgt gggcatcccg actgaagaat
     1801 gacctcaaaa tccgtctgcg ggctagcgat cgctgttata ttccttccct ttctcggaga
     1861 ctgcgctact caattctccc cgacaaccct gtccattgta tctgacaaaa acctggagca
     1921 aatggagaag cccgttcttc ttgcttccgc gacacaatct caatgtcgcg ttgtgctgca
     1981 gcaaagagta ggatacgtac tcttccactt gccgttcaat gaaatcacaa gcggacaaat
     2041 caaatccgca cttcaatcgc aaaaggaacc atattccatt cgctacaggg aaatgctcac
     2101 gccggcagat attggtctga acatcctcgg ttttctattt agcgtgctga ccgtgaccaa
     2161 acaagtagaa gcatgcgcag cacctgagat ggtggaaagc tccgaccagg caaaaaaagc
     2221 aagaatctac tcagaatttg agaaggaaag aaaaaggttc tcgcggtttg aagtgcccat
     2281 cggtcttacc acaacaatcg aaaaaaaagt ccgccacaga gtttatcaaa aaccagagga
     2341 cggagaagca gctatgatct ccagggaagg atcgctcacg atcaagtcca acaaacttca
     2401 acttgaaacg gaagagggta cagtggcaat ccccgaagat tccgttctca tgatagaggt
     2461 catgattccg gaaggctaat caatcaatgt gaagtaagaa tcctttgagg tagtcacctt
     2521 cggggtagaa gatgcttaaa ggatggtccg ggctctggcg atgggattga aggatccgaa
     2581 catccctcct tgcctccagt gcggcctgga aaatgatttt tctcatgaga tcactgtcca
     2641 caggatgaga gcaagaactg gtgagaacca gaccgccttt ttcgatgacc tgaaatgcaa
     2701 gcctgtggat gtcgcgatac ccgcgcattg cctgaggaag atctctcttc gtgcgagcaa
     2761 aggcaggtgg atcaaggacg atgatgtcaa attcttcttc cacggaccgc aagtattcaa
     2821 agacatcgga agcctcaaat ccgtgattgg cctgcgaaat ctgattgaga gaaaagttct
     2881 cctttgccag agcaattgct ttttctgaac tatctacgga gacaacgcga gatgctccac
     2941 cggatgcagc atacacgctg aagccgcccg tatagctaaa gcaattcaag acccgcctat
     3001 ttctagaaag agtgcgaagg taggcgcggt tctctctttg atccaggaaa aagcctgtct
     3061 tctgggatcc ctcaaattca acgcgaaagg aaagaccgtt ttcctgaatc gttacagagt
     3121 ccacaccgct cccataaatc caggcgcttg actccagtgt tccaggttcg gcagaagtac
     3181 gcggcacaac cttttgatac atgcctttgc actttactcc agctttcgcg gatgcagatc
     3241 gaaacgcaga tatgagatgc tctttgtttc gctccatccc gacggatgag atctgaatga
     3301 cccaatattc cgcataacgg tccagagtga ctcctggcat accgtctgat tctccagcca
     3361 cagccctcca cgcattgccg tctaacggta aagaagtgcg caggcccagg gccgccacaa
     3421 agcgatccac ggcccactgc tgaaaatcag tgtcctccgg agattctgca aaccagagca
     3481 tccggccggc aagagaagca cccttgcgaa attggccaca tcccagatac tcgccggaat
     3541 gactttgtac gtttagaagg tccccatctt cgaatgtagg ccactttgcc accgcacccg
     3601 aaaaaaccag aggatgcctt tgtttgatcg acttctctct gccgggttga agcacgatgc
     3661 gcggattgct gttcactgga acaaagagct ggacaacgct cgcacgtcaa acgaatgcgt
     3721 gtgatgccca gattgactcg ctattttgta aaatccgcgt tcatctgcct ggttctgtct
     3781 ttcatgaccg gaatcattct ccatctggac tttctgattc caaacgcagg tgtgcttcgc
     3841 cctgtttttt atcatctatt cctggtggga ttcacaactc agctgatttt cggaatctcg
     3901 tactggatgt ttcccactct ttcgcgagac aatctaaggg gcaacgaatc gctcggatgg
     3961 atttcttacg tcgctctgaa cggaggcttg ctgcttcgcg ccacggctga accggcagta
     4021 gcactttctc cggtgccggc gctgcgaatc actctggccg cggccgcgtt cttgcaactc
     4081 atcgccgtgg ttgcttacgt tgctttgatc tggaagagga cgaaggcaaa ataatgccag
     4141 ccatcagtcg tatcctcgtt cgcacatctt tctgttatct gattctgggc tctgcgattg
     4201 gaacctggct cttgctcgat aaagctgctt ccactccaat cctcgagggc gtgcgaaaca
     4261 tccaccgcga aatcatgctg gccgggtgga ttgttcagct tgtgatcgga acagccacct
     4321 ggatcttacc gagaaatgac cggagtcgcg gcgatatgaa aagtgtatgg atcgcctacg
     4381 cgctgttcaa cgctggcatc ctggtggcgg caatcggaac tattcttgga aatcaagccg
     4441 tcctgacggc ggcacactgt tgctatgcgg cgggggctat ttcttttgcg aagcatgcat
     4501 ggccgcgaat caaaagcttg ccgcaggatc aagaaagacc aaacgctcgc ccaccctgat
     4561 agatcaggag agcgaacaaa tatgcaaggc cagtcatata agcgaacatg aatccggccc
     4621 atccccagct ctgcaattct ctctgaacag tcacaaccgt actcatgcac tgacaacaga
     4681 gcgcgaagaa aaccatgagt gagacagcaa ggagaggagt aaacaaagta gaaccatccg
     4741 ctctcttttc gagtgcc
//
LOCUS       DAORFF010000285         4757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10246_length_4757_cov_3.755849, whole genome shotgun sequence.
ACCESSION   DAORFF010000285 DAORFF010000000
VERSION     DAORFF010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10246_length_4757_cov_3.755849"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1089)
                     /locus_tag="PKK76_11805"
     CDS             complement(28..1089)
                     /locus_tag="PKK76_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69205.1"
                     /translation="MRSIQLPVRVWLLLCFALSFCGNDSQPLAEWEGGSITRQELKYT
                     FQLLGKPPKLDSQEKVLEGLAVLKMSASESLRQGLADSPEFQKRSVFFEKTAFVGAFE
                     QFVAQETRKKKFKMMELQGAFLHDAATPKTREEEALDFVKRLNAASEIDVIQMIAERT
                     ELRRYGVVGGYMEPLCSNCPMNPYVFLINPARRAEGKFISIPDSNGFWIVRKVREEEF
                     ADDDLVEEYAHYYRKIVPIGRHFVRELPEGNERLALEVTIPADSDIAMTANQKAQAHL
                     VRESRLAYENKLREKKAEHHFEAGPNWNKSDQIANRAELLFKLDEKSFTLADFETEIP
                     ESARDKGLILHQVLFPYLL"
     gene            complement(1104..2054)
                     /locus_tag="PKK76_11810"
     CDS             complement(1104..2054)
                     /locus_tag="PKK76_11810"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNL69206.1"
                     /translation="MRTLKHIVEWSFVRSLLFFFGLLPYEKGKVWMGAVTGWLGMTFA
                     RKLQRRMMDVLRIAFPEHSEERNRQIIRTTFENLGHQAVEFLTLEKMDQAWVDKYFEF
                     EPTGAEAVRKAVREKKGQLFVLAHTGNWEMLNQPMTIHTGVHINLIIAPMSNPFGDKF
                     LCNIRRSLGTTLIYPFDVGPNVLKALARGEAAAFASDQNAGRRGLFIPFLGQPASTHT
                     GAAVFSYLSGAEIFFITNMRLPNGRFRTEILPMGSIDKKKVKDKDECIKEFTLRYVDI
                     LEKYIRKYPDQYFWLHNRFKTKPLPEDEIIPRRDFDLKPY"
     gene            complement(2051..3148)
                     /locus_tag="PKK76_11815"
     CDS             complement(2051..3148)
                     /locus_tag="PKK76_11815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNL69207.1"
                     /translation="MALRVKLGLLELALNRFASDFLRLISICAVLVIHSTGEFERSFA
                     SSHRFFSAEFVGIVLNQLARFSVPVFIMLSGFALSLRYKGDLDVREFFVRRASKIGIP
                     FLAWTPVFLLAHQIGQHPDLMVSASGWPDALVSALGELPAALFTYGADYHFYFFIIIL
                     QCYLLFPLLLRASGKVWWILIPLHLITHAPAHLLLDQIGVHLPRFPSSFFVYWILYFF
                     AGIRFARNAKQVHPAVALLFFIPVILEFMFYSTRDTETGNYDHFTRWTVTLFAFAVFC
                     AAHWLAKFENRAAEIVTKGAGISFAVYIIHTTVLRILSMTPLSGNLLIIPALIAVTFS
                     FCAFLDRVIGVSWLRTVFGLPEKMIPKGATQ"
     gene            complement(3161..3997)
                     /locus_tag="PKK76_11820"
     CDS             complement(3161..3997)
                     /locus_tag="PKK76_11820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HNL69208.1"
                     /translation="MSEDLSVFEWRRKLHEALHGVGIEAADLEADLILRDILNLDAAG
                     LILNKNNLVPADKTVRLTAVMHERLKRRPLAHVLGYTEFYGRRFRTDARALVPRKETE
                     LLVEQLLLAPLPAGPVLDLACGTGIAGITIALEKPGTIVHCSDLSEDALSLARENALA
                     LKAKVEFYMSDWFESVPDVRYAAILCNPPYIWLRDSNSLEPEVLFDPAMALFHENPPA
                     LYEHLLGEAKMRLVRGGFVFFELGADIAHAVLERAQKIFVNARILADYSGLPRMLIAS
                     TE"
     gene            complement(3994..>4757)
                     /locus_tag="PKK76_11825"
     CDS             complement(3994..>4757)
                     /locus_tag="PKK76_11825"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HNL69209.1"
                     /translation="DRLPRAPARSLIVKCVSSLDMAEAEWDGLVRLREAGAVVPELIG
                     FVKTDSGGRSGALLFLSMVSGGRGSVRLESLIQIYSTRGESYGYSRDNYIGSLHQRNR
                     RYATFEEFWAQDRIFPQLNMAISAGLLTQKDLENARRVLDIASGWELSQPGPRLIHGD
                     LWNGNLLIDSDMNPVLIDPSVSYSHPEQDLAMLELFGSPLSSSDLDLLARETGLPAGR
                     IERRDFFQLYPLLVHVNLFGASYVSQARRAIGRYL"
BASE COUNT         1298 a         1233 c         1144 g         1082 t
ORIGIN      
        1 tttcactgaa caaggactca cttgaattta tagaagatag ggaaaaagaa cctgatgcag
       61 aatcaggcct ttgtctctcg cactctcagg aatctcggtc tcaaaatctg cgagagtgaa
      121 tgatttctcg tccagcttga agagcaactc tgcgcgattc gcaatctgat ctgatttatt
      181 ccagtttgga ccggcctcaa aatggtgctc cgcctttttt tcgcgaagct tgttttcata
      241 agcaagtctg gattcgcgaa ccaggtgcgc ctgcgctttc tggttagctg tcattgcgat
      301 gtcactgtct gctggaatag ttacttccag ggccagcctt tcatttcctt ccggcaattc
      361 gcgaacgaag tgtctgccaa tcggcacaat ctttcgatag tagtgcgcat attcttccac
      421 cagatcatca tccgcaaact cttcctcgcg aacctttcgc acgatccaga atccgttgct
      481 atccggaatg gaaataaact tcccttccgc tcttcgcgcc ggatttatta gaaatacata
      541 cggattcatc gggcagttgg aacaaagcgg ttccatgtag ccgccaacta cgccatatcg
      601 ccgcaattct gtcctttcgg cgatcatttg aatcacatct atttcactgg ccgcattgag
      661 ccttttgaca aaatcgagag cttcttcttc gcgcgtcttt ggagtggcgg catcgtgcaa
      721 gaaagcacct tgcaattcca tcattttgaa tttcttcttt cgcgtttctt gagcaacaaa
      781 ctgctcaaag gcgccaacga acgcagtctt ctcaaagaat acagaccttt tctgaaactc
      841 aggcgaatcc gcaaggccct gccgaagaga ctcggacgcg gacatcttga gcactgccag
      901 cccctcgaga actttttcct gcgaatctag ttttggcggt ttccccagaa gctggaatgt
      961 gtatttcaac tcctgccgtg tgatactacc gccttcccac tctgcaaggg gctgcgaatc
     1021 attgccgcaa aacgaaagag caaaacaaag aaggagccaa acgcgtaccg gcagctggat
     1081 agaacgcata tgcttcttga tcctcagtag ggtttcaaat caaagtcgcg gcgtggaatg
     1141 atttcgtctt cgggcaatgg ttttgttttg aaacgattgt gaagccagaa atattgatcc
     1201 ggatattttc tgatatattt ctctaagata tcaacgtaac gcagagtaaa ctctttgatg
     1261 cactcgtctt tgtccttcac tttctttttg tcgatgcttc ccatggggag gatctctgtg
     1321 cgaaatcgtc cattgggcaa acgcatgttc gtaatgaaaa agatttcagc accgcttaaa
     1381 tatgaaaaca cagcggcccc ggtgtgcgtg gaagcgggct ggccaagaaa cggaatgaaa
     1441 agtccgcgac gccctgcatt ttgatccgac gcgaaagcag cagcctctcc acgagccaat
     1501 gccttgagga cattcgggcc aacatcaaat ggataaatga gagtagtgcc gaggcttcgc
     1561 ctgatgttgc atagaaactt gtccccgaac ggattggaca tgggtgcaat gatcaagttg
     1621 atatgaacgc cggtgtgaat ggtcatgggc tgattgagca tttcccagtt tccagtgtgc
     1681 gcgagtacaa agagctggcc tttcttttcg cgcactgctt ttcgcactgc ttccgcgccc
     1741 gtgggctcga attcaaagta cttgtccacc caggcctgat ccattttttc cagtgttaaa
     1801 aattcaacag cctgatgacc cagattctcg aacgtggtac gaatgatttg cctgtttctc
     1861 tcttcgctgt gctccgggaa tgcgatgcga agcacgtcca tcatgcgacg ctgcaacttg
     1921 cgcgcgaatg tcattccaag ccatccggtg accgcgccca tccacacttt ccctttttca
     1981 taaggaagaa ggccaaaaaa gaacagcaac gatcttacga acgaccattc tacaatatgc
     2041 ttgagtgtcc tcattgcgtt gctcctttcg gaatcatttt ctctggaagc ccgaacaccg
     2101 ttcgcagcca ggatacgcca atcacgcggt ccagaaaagc acaaaacgag aatgtaaccg
     2161 caatcaaagc aggtatgatc aacaagtttc ctgaaagcgg cgtcatggaa agaatccgca
     2221 gcactgtagt atgaataata tagacagcaa aggaaatgcc tgcgcctttc gtcacaattt
     2281 ctgctgcgcg attttcaaat ttagcaagcc agtgagcggc acaaaaaacc gcgaaagcga
     2341 ataacgttac agtccagcgc gtgaaatgat catagttgcc tgtttccgtg tcccttgtgc
     2401 tgtaaaacat gaactccaga atcacgggaa tgaaaaacaa aagtgctact gcaggatgaa
     2461 cttgctttgc atttcgcgcg aagcggattc ctgcaaagaa gtacaaaatc cagtacacaa
     2521 aaaaggaaga ggggaatcgc ggaagatgca ctccaatctg atccagaagt agatgcgccg
     2581 gtgcatgcgt gatcaagtga aggggaatca ggatccacca cactttcccg gacgcacgca
     2641 aaagcagagg gaaaagcaga taacactgaa gaataataat aaagaaatag aaatgatagt
     2701 ccgcgccata cgtgaataac gccgccggta gttctcccag tgcggacacc agagcatccg
     2761 gccaaccgga tgcggacacc atcaggtctg gatgctggcc aatctgatgt gcgaggagaa
     2821 acactggagt ccacgctaaa aatggaattc caatcttcga agcgcggcgt acaaagaact
     2881 cccgcacatc cagatcccct ttgtagcgca acgacagagc aaacccggac agcatgatga
     2941 aaacaggaac tgaaaagcgt gccagttgat tgagaacaat ccctacaaac tctgcagaaa
     3001 aaaagcgatg gctcgaagcg aaggatcgct caaactctcc cgtgctatga attacaagga
     3061 cggcgcaaat ggaaatcagg cgaaggaagt cggacgcaaa acgattcaaa gctaactcca
     3121 gcaggcccag cttgacgcga agagccacac actcaatcca ttactcagtg cttgcaatca
     3181 acatacgggg caacccggaa taatccgcaa gaattctggc atttacgaag atcttctgtg
     3241 cgcgttctag cacagcgtgc gcaatgtcag cgccgagctc aaaaaagacg aacccgcctc
     3301 tgaccagacg cattttcgcc tctccaagca gatgctcgta cagtgcggga ggattctcgt
     3361 gaaagagagc catcgccgga tcaaataata cctccggttc gagagagtta ctgtcacgta
     3421 accagatgta cggtggattg cataggatgg ccgcataacg tacgtctggg acggattcaa
     3481 accagtcgct catatagaat tcaactttcg ctttcagtgc aagtgcattc tcacgggcca
     3541 gggacagggc atcctctgat aggtcactgc agtgcacaat agtaccaggc ttttcaagcg
     3601 caatcgtaat gcctgcaatg cccgtcccgc acgcaagatc gagaactggt ccggcgggca
     3661 gaggagcgag taacaattgt tctacgagga gctctgtttc ttttcgcggg accagggcac
     3721 gtgcgtccgt gcgaaatcgg cgaccgtaaa actcagtata tccaagaaca tgagcgagcg
     3781 gtcgacgctt gagtctttcg tgcattactg ctgtaagtcg aactgtcttg tcggcgggaa
     3841 ccaggttgtt cttgttcaag atcaaaccgg ctgcgtcgag attcaggatg tctctaagaa
     3901 tcaaatctgc ctcgagatca gccgcttcaa tacccacacc atggagggct tcgtgcaatt
     3961 ttcggcgcca ttcgaatacg ctcagatctt cgctcacaga taccgtccga ttgcgcgacg
     4021 agcctgcgaa acgtaagacg cgccaaacag attcacgtga acgagaagtg gatagagctg
     4081 aaagaaatcg cgcctctcta ttctgccggc cggaagaccc gtttcgcgcg cgagcaaatc
     4141 cagatcagaa gaagaaaggg gagagccaaa aagctcaagc atggccagat cctgctcggg
     4201 atgagagtaa gaaacggacg gatcaatgag gactggattc atgtctgaat ctatgagcag
     4261 gtttccgttc cataaatccc catgaatcag tcgcggacct ggctggctca attcccatcc
     4321 agacgcaatg tccaggactc gtcgcgcatt ttccagatcc ttttgggtca aaagcccggc
     4381 agagatcgcc atgttgagct ggggaaaaat acgatcctga gcccagaact cctcgaaagt
     4441 cgcataacgt ctatttctct gatgcaggct gcctatgtag ttgtcgcgcg aataaccata
     4501 agattcacct cgggtagagt agatctggat cagtgactca aggcgaacgg aacctctgcc
     4561 accggaaacc atcgacagga aaagaagagc gccggatcgt ccacctgaat ccgtttttac
     4621 aaaaccaatc aattcaggaa ccaccgcacc cgcctctcga agtcgaacca gaccgtccca
     4681 ctccgcttcc gccatatcca gggaagatac acactttaca atgagagatc gcgcaggcgc
     4741 gcgcgggaga cgatcgc
//
LOCUS       DAORFF010000286         4731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10319_length_4731_cov_3.893071, whole genome shotgun sequence.
ACCESSION   DAORFF010000286 DAORFF010000000
VERSION     DAORFF010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10319_length_4731_cov_3.893071"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PKK76_11830"
     CDS             <1..428
                     /locus_tag="PKK76_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HNL69210.1"
                     /translation="FQIANPAATIAWLVLVLFPGWKYVRVFTVTIMSSLVFAGVYAFA
                     LGTSMAAGGSGNFSSLEGVSMLFQNPYALLAGWVHYLAFDLFVGSWISFHAQKLGISR
                     WIILPSQFLTFMFGPIGLFTFLLIRALMRRESLATDPFA"
     gene            511..1821
                     /locus_tag="PKK76_11835"
     CDS             511..1821
                     /locus_tag="PKK76_11835"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNL69211.1"
                     /translation="MFIRLALILILLIAQACRRPIDFGPDNPGGLATVSATGESAFSL
                     PAANIDPRWIVKFARGNRSFKTPWSDQAGAEQPGLGPLFNHTSCAGCHPRDGRGQPPK
                     NPGDLDHGMLIQLGDSGKNHPLYGDTLQTRAIQGHNPEPSFRIRYEEIKGEYADHTPY
                     SLRKPIVEFNVQSETIPAFSARTAPALIGLGLLESISESEILIRSQRVKDVNPAIGGR
                     MNHVRNGDGDEIPGRFGWKASVARIEDQIARAAFRDMGVSSRLLPASLDSATAGNASE
                     PEMKDDVLANITFYIRSIGVPARRDPWNPDVQRGEEVFLRLGCANCHAETFVIGRTSL
                     DHLTYQVIHPYTDLLLHEMGPGLADHRSDGEATGSEWRTAPLWGIGLVQTVNGHEFFL
                     HDGRARGFEEAILWHGGQAEASRNGFLSLAARDRALLLQFLRSL"
     gene            complement(1824..4616)
                     /locus_tag="PKK76_11840"
     CDS             complement(1824..4616)
                     /locus_tag="PKK76_11840"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNL69212.1"
                     /translation="MRRHLATVFDRLFFVVGILILAVSAGLISVAQVLKRPWMYAWMV
                     SEDSLTVGRAETRTRQKFSEPHATYRWWQLRTTGKRYFQAERFADGLAHVQIDREMGY
                     IDTKGKPAFTARFNDGEQFHNGRAVVRVGYHYGVIDRQGEFVIAPVHFAISHLLTPDR
                     RLSFFSVSNSEYGAPQLLDANGKLLLDAAYRVTIGPRSLIAQGNKKVGADYEDFFAIY
                     DHAGRIISEQIGDFYNSRGIFVQNHLWGVMNDQGEITVAPSFNYLQFLQNGNIYGETE
                     CKPSLIGRDGTRTESMPLFKGTREENPNGHVYCEYMQDASRCPCVYSGGIITCNINDT
                     TKFRKPLNYIGPFSRGLAIAKRTANEPFGFVNESGEFVIAPQFDSAIEPDACFAYARK
                     DNAVFAIDPVTLQIRPALLSNPGRLQFKDGIACGEMESAQGQGLYANSNRTGCVNESG
                     TIQVPPRFWRIGEFSEGVAFAQTLGGNSGLIDINGKWISVLPALDIEPFSSDLAQFTD
                     STGYHGYIDRRGNVSLPPVYESAGNFNDGVAPVRRRSEKTTYIRKDGSRAFQNDFELG
                     YDFSEGLAAVSWSTNEAFIDRTGKVVFGGKYRYGSRFSEGLVSYSDSGSHLMGYVDRS
                     GNVQIPARYTAAYDFKEGLAAVKQACGTENCPFGYVDQSGQVRIAPHSNLTGAPFYNG
                     RATIQNDKMKFGLIDPSGAIIVSPIYDSLEPAGAGLYRARREGKAGFIDRNGKIISGK
                     FYQDARLFREGLAAVQSDSGFYDFIDPQGNKVIETGFSDVGNFSCGLALFGFTGSGYN
                     QKPPDAFERFYYPSKGSFFSKGEGKGQKADGGDYFRLDRYEAQVRYGFLNKTREVQIP
                     PMFERAATFGIENEFEDRGEMDSETRKCISHVTAPDRKNAYYMDVHGDRIGDRPDYYP
                     YILYLLGQYQDWLTKP"
BASE COUNT         1071 a         1182 c         1215 g         1263 t
ORIGIN      
        1 tctttcaaat tgcaaatccg gccgcgacaa ttgcgtggct tgtcctggtg ctgtttccag
       61 ggtggaaata cgttcgtgtt ttcacggtga ccatcatgag ctctctagta tttgcaggcg
      121 tctatgcatt cgcgctggga acgagcatgg ctgcgggcgg aagcggaaat ttcagcagcc
      181 tcgaaggagt gtccatgctg tttcagaatc cgtacgcact tctggccggc tgggtgcact
      241 atcttgcgtt tgatttgttt gtaggaagct ggatttcttt tcatgcgcaa aagctgggta
      301 tttctagatg gatcattctt ccgtctcagt ttctgacgtt catgtttggg ccgatcggtc
      361 tgtttacttt cttattgatc cgcgcactta tgaggcgcga atccctggcc acggatccgt
      421 tcgcttgaga caaatcagat cggcgcgctc ttgagatgag cgcgccccct ttgtgattgc
      481 gtcctgggcg gaactttcct aatcaggatc gtgttcatcc gacttgcgct gattctgatt
      541 ctgctcatcg ctcaagcatg caggcgcccc attgattttg gcccggataa cccgggcgga
      601 ctggccaccg tctcggccac tggagaaagc gcattttctt tgcccgccgc caacatcgat
      661 ccacgatgga ttgtaaagtt tgcgcgggga aaccggagtt ttaagactcc atggtcggac
      721 caggctggcg ccgagcaacc aggcctcgga cctttgttca accatacttc atgcgccgga
      781 tgtcatccgc gagatggtcg tggtcaacct ccgaaaaatc cgggtgacct ggatcacgga
      841 atgttgattc aactgggaga ttccggaaag aaccatccgt tatacggcga cactctccag
      901 actcgtgcga tccagggtca taatcctgaa ccgtctttta gaattcgcta cgaggaaatc
      961 aaaggagaat atgcagatca cactccgtat tcgcttcgta agccgatcgt tgagttcaat
     1021 gtgcagtcag aaacgattcc tgctttttca gcgcgcacag ctcccgcttt gatcggactg
     1081 ggtctactgg aatccatcag cgagtctgaa atcttgattc gttctcagag agtgaaggat
     1141 gtaaatccgg cgatcggcgg cagaatgaat catgtgcgca acggtgacgg agacgaaatt
     1201 ccaggtcgtt ttgggtggaa ggcaagtgtg gcgagaattg aggatcagat tgcccgcgcg
     1261 gcctttcgtg acatgggtgt atcatcccgg ctgttgcctg cttccctgga ttctgcgact
     1321 gccgggaatg cgtccgagcc cgagatgaag gatgatgtac ttgcgaatat tacgttttac
     1381 atacgatcta tcggagtgcc tgcgcgaaga gacccctgga atcctgacgt gcaacgaggc
     1441 gaggaagtgt ttcttcgact gggctgtgcg aattgccatg cggaaacatt tgtcattggt
     1501 agaacttcac ttgatcatct aacctatcag gtgatccacc cgtacacgga tttgttgctg
     1561 catgaaatgg gcccggggct tgcggatcat cgcagcgatg gagaagcaac gggtagcgaa
     1621 tggagaacgg ctcctctctg gggaattggt ctggtgcaga cggtcaatgg ccatgaattt
     1681 tttcttcatg atggtcgtgc gcgtggcttt gaagaagcga tcctgtggca cggcggtcag
     1741 gcggaagcgt ctcgaaacgg ttttctttct ttggccgcgc gcgatcgagc acttctcctg
     1801 caattcttgc gcagtctcta ggtctaaggt tttgtcagcc agtcctgata ctggcccagc
     1861 aagtagagaa tgtaagggta gtagtcaggt cgatccccga tccgatctcc gtgcacatcc
     1921 atgtagtacg cattttttct gtcaggcgca gtcacgtgag agatgcattt tctggtttct
     1981 gaatccattt cccctctgtc ttcgaattcg ttttctatgc cgaaggtggc tgcgcgttcg
     2041 aacataggcg ggatctggac ttctcgagtc ttattcagga agccataccg cacttgtgct
     2101 tcatatcgat cgaggcgaaa gtaatcacct ccatctgcct tctgcccttt gccttctcct
     2161 tttgagaaga atgatccttt gctcgggtag taaaagcgtt cgaacgcatc cggcggcttc
     2221 tgattgtagc cggatccggt gaacccaaac agggcaagtc cgcacgaaaa atttccaaca
     2281 tctgagaagc ctgtttcaat gactttgttt ccctgtggat ctatgaaatc gtaaaagcca
     2341 gagtctgatt gcactgctgc cagaccttct ctgaacagac gcgcatcctg ataaaatttt
     2401 ccagaaatga ttttcccatt cctgtcgatg aatcccgctt ttccttcgcg ccttgccctg
     2461 tacaatccag cacccgccgg ctccagtgag tcataaatcg gagatacaat gatcgcgccc
     2521 gatggatcga tcagtccgaa tttcatttta tcattttgaa tggtcgccct tccgttataa
     2581 aagggagcac ctgttagatt ggaatggggt gcaattcgaa cctgaccact ctgatcgacg
     2641 tagccaaaag ggcaattttc agttccacag gcttgcttca cagcggcgag tccctccttg
     2701 aaatcatatg ccgcagtgta acgtgcggga atctgtacgt ttccggatcg atccacgtat
     2761 cccatcaaat gtgagccaga atcagaataa cttacaagtc cttccgaaaa gcgagaccca
     2821 tagcgatatt ttccaccgaa tacgactttg cctgtgcgat cgatgaaggc ttcatttgta
     2881 gaccatgaaa ctgcggcgag accttcgcta aagtcataac ccagttcgaa atcgttctgg
     2941 aatgcgcgac ttccgtcttt gcgtatgtag gtggtctttt ctgatctgcg gcgaaccggt
     3001 gcgactccgt cgttgaaatt cccggcgctt tcatacacag gagggaggct cacgtttccc
     3061 ctgcgatcga tatatccgtg atatccggtg gaatcagtaa actgcgcaag atccgatgaa
     3121 aatggttcta tatccagcgc aggtaagaca gaaatccatt ttccgtttat gtcgatcaat
     3181 cctgaattgc cgcctaacgt ctgcgcgaag gctacgcctt cgctaaattc gccgattctc
     3241 cagaagcgag gcggcacttg aatggtgcct gattcattta cgcaaccggt tcgattggag
     3301 ttggcataga gaccctgtcc ttgagccgat tccatttcgc cacatgcaat tccgtctttg
     3361 aattggagtc gtccgggatt ggatagaagt gcagggcgaa tttgtagtgt tacaggatct
     3421 atcgcgaaga ccgcgttgtc tttgcgtgcg taggcaaagc aggcgtctgg ttctatggca
     3481 gagtcgaatt ggggagcgat tacaaattca cctgactcgt tcacgaatcc gaaaggttcg
     3541 tttgccgtgc gtttcgcgat ggcgagccct cgcgaaaatg gtccaatgta gttcaaaggt
     3601 ttccgaaact tcgtagtatc gttgatatta caggtaatga ttccgcctga ataaacacac
     3661 gggcatctgg atgcatcctg catgtattcg cagtacacat gcccatttgg attttcttct
     3721 cgcgttcctt tgaatagcgg catggattcg gttcgcgttc catcccttcc aattagtgag
     3781 ggtttacact cagtctctcc atagatattt ccgttttgga gaaattgcag atagttgaat
     3841 gaaggggcta ctgtgatctc gccttgatcg ttcatgactc cccataagtg gttttggacg
     3901 aaaattccgc ggctgttgta aaaatctccg atttgttccg aaatgattct gcccgcgtga
     3961 tcatatatgg caaagaagtc ctcatagtct gcacctacct tcttgtttcc ctgtgcgatc
     4021 aaagagcgag gtccaattgt gacacgatac gcggcatcca ggagtaattt gccatttgcg
     4081 tctaacagct gcggggcacc gtactcggag ttggagacag agaagaagga gagacgacgg
     4141 tcgggcgtga gaagatgaga aatggcaaag tgcaccggcg caatgacgaa ttcgccctgt
     4201 ctatcaatga cgccgtagtg gtaaccgact cggaccacag cccttccatt gtggaactgc
     4261 tctccgtcgt tgaagcgcgc ggtaaatgca ggctttccct tcgtgtcaat gtagcccatc
     4321 tcacggtcta tttgcacatg cgccaggcca tctgcaaaac gttccgcctg aaaatagcgt
     4381 ttccccgttg tgcgcaattg ccaccaccga taggtggcgt gaggttctga aaacttttga
     4441 cgtgttcttg tttctgctct acccactgtg agcgagtctt ccgaaaccat ccaggcatac
     4501 atccagggtc gttttaaaac ctgtgcgacg gaaatcagcc ctgcgctgac tgcaagaatc
     4561 agaataccaa caacaaagaa tagccggtcg aatacggttg caaggtgtcg gcgcaaagta
     4621 gtgcatcttt ggctgagtca aaatatccag tcaatcattc tggctttgtg attgcacggc
     4681 tcgttcgcat tatttccatg catgcaaata tgcagatcta tcacaacccc a
//
LOCUS       DAORFF010000287         4721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10360_length_4721_cov_3.527861, whole genome shotgun sequence.
ACCESSION   DAORFF010000287 DAORFF010000000
VERSION     DAORFF010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10360_length_4721_cov_3.527861"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PKK76_11845"
     CDS             <1..559
                     /locus_tag="PKK76_11845"
                     /inference="COORDINATES: protein motif:HMM:NF018534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNL69213.1"
                     /translation="PFWSAVKTGTSKDMRDNWCVGYSDRYTVGVWVGNASGEPMWNVL
                     GVTGAAPVWQEVMRVLHADSPSRAPGLPDSVAIQATEYGKEYYIKGTEPAHFHEIEPR
                     DPRILYPESGTIFALDPEIPPERQRIFFRAASVQSRLQWKLDGKVIGEQNPAVWKPAR
                     GSHKLTLLLCEHVCAETQSVGFMVR"
     gene            769..1419
                     /locus_tag="PKK76_11850"
     CDS             769..1419
                     /locus_tag="PKK76_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69214.1"
                     /translation="MFGALPINKVDLSLQETGKTYRVKEESKWYDVLIDVFGGAILTL
                     TRKTITIDSCDERLIVVSQDELETERKKELQAALDKYATETPTLPEEAIFILSSGDSI
                     RGKILELDSDTITIAVRSTVEQASQVDSVNAASIILRDGKTVVGEVIGQDNQSISLKV
                     GGRTVVIQKERIKRISYQRASTEKEGKPETQVEEVVLPRAQIKRIVLNRPPNGAVN"
     gene            complement(1448..2038)
                     /locus_tag="PKK76_11855"
     CDS             complement(1448..2038)
                     /locus_tag="PKK76_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNL69215.1"
                     /translation="MHNLVRDHIERSIDVKKMLLEKETDRISAAGQALAELMQKGGRL
                     FTCGNGGSAGDAQHIATELLIRYRSAPERRSLPAISLVGDAAAVTAAGNDFGFEYVFS
                     RQIEGLGSQGDAILAISTSGNSANIIQALHAARKKHMMTFVLTGGSGGQIAKEGNLAD
                     HIIVVPTAETARIQECHIMIGQIFCAIIERQLFGFE"
     gene            2094..2648
                     /locus_tag="PKK76_11860"
     CDS             2094..2648
                     /locus_tag="PKK76_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69216.1"
                     /translation="MAENPRLIAALDFILNHATGAELEALASALSRRGNSSASSLNFQ
                     DMASSVTRGLSGRFAMPENMNEVTRNMVKGMIRQHEPNLSPEQIDVLLDQWVPSPQQV
                     RQGQEGKYPPEVLYSMAEQFLMYGIGNMPDSEARELKEAMPDWHEKYWNIFSPVIRTL
                     LADAIRGNLGLSECRARLKAHLRL"
     gene            complement(2679..3602)
                     /locus_tag="PKK76_11865"
     CDS             complement(2679..3602)
                     /locus_tag="PKK76_11865"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL69217.1"
                     /translation="MRSALFSLILLTACASAQDMFLQGVNAEQKNNSREALDYYAAAL
                     AKDPSMAKAYNNRGLILYKDKQYQDAIAEFDQALKIKPDYAAALSNRGLVLEAMNDPK
                     AAMAQYEKAVSLDPKFPEAYFNAGALSSKNGDILHAIQWLEKANELKADDGETLLLLG
                     RALLVAKNYGRAAQMFDALLKVDPNSRDAFIGSVDATRLAGNQDVAAARLNQYVAMHP
                     DCALCSSMLGAIYTDQKKYDSAIEVFEKSRQYRPGDPTPRYHLGFLYFLTKNKSKSIE
                     NLEEFLKLKGDFKDAQTEKASQMLAALKKGS"
     gene            complement(3599..4288)
                     /locus_tag="PKK76_11870"
     CDS             complement(3599..4288)
                     /locus_tag="PKK76_11870"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNL69218.1"
                     /translation="MKTVLVFAFLFVLQCAKAPEGAIISQVRGTASVEIQGKSEPATV
                     GKKVNAGDVIITEKDSEVDLQIAGGGAFRVLNASRITVGALAQKSEINVQKGGLLLGL
                     KKMKADEEFTVRSPTALAGVRGTSFSFSADRNTIAVLTGSVQVERQGKALQLEPFQEI
                     QTDKEKLESKKLSPDSARQLARIGEMKGIEDLADFDEIQKNLSVVILEIEKENLKTGK
                     TVAPRPRPEVQ"
     gene            complement(4310..4570)
                     /locus_tag="PKK76_11875"
     CDS             complement(4310..4570)
                     /locus_tag="PKK76_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69219.1"
                     /translation="MRKHKLPWTEPLYLDGHILICVPENHLHFAEHFLNDLIQLIWII
                     YPSGIAAHKFQVTNLPWKGKQSMQLKWEPGWYVRWKKLPCLV"
BASE COUNT         1123 a         1142 c         1246 g         1210 t
ORIGIN      
        1 acctttttgg agcgccgtga aaaccggcac aagcaaggac atgagagata actggtgcgt
       61 tggctatagc gaccgttaca cggtcggtgt atgggttggt aacgcaagcg gcgaacccat
      121 gtggaatgta ctgggagtca ccggtgctgc gccagtctgg caggaagtta tgcgggtgct
      181 ccatgcagat tctccgtccc gcgcgcccgg tctcccggac tcggttgcaa tccaggcaac
      241 ggaatacggg aaagaatact acatcaaagg cacagaacca gcccattttc atgagatcga
      301 accgcgtgat cctcgcattt tgtatcctga atcaggaacg atattcgccc ttgatccgga
      361 aattccgcca gaaaggcaga ggatattttt tcgagcggct tccgtgcaaa gcagacttca
      421 atggaaattg gacggcaaag tgattggtga gcagaatcct gctgtctgga aaccggcgag
      481 gggtagtcat aagctaacat tattgttatg cgaacacgtc tgcgcagaga ctcagtccgt
      541 aggattcatg gttcgttgat cccttggaat ttactgcttg cggtgagggg gcgccagctt
      601 ttgaatcaac tcatgaggca ctctttgatc gtcctccttt ccgcaattct ggttggggca
      661 tgttcaacaa catctgtgca aatgcagagc ccggctaacg gcacgcgaac cgtgctggcg
      721 tctccgcaaa acagctgtca ggcgacgacg cgcggacagt ggtacttgtt gtttggcgcg
      781 ctaccgatca acaaagtaga tctttccctg caagaaacgg gtaagacata cagagtgaag
      841 gaagagtcaa agtggtatga tgtgctcatc gatgtcttcg ggggcgccat tctcaccctt
      901 acgcgaaaga caatcacgat cgattcctgt gacgaaaggc tcattgtcgt ttctcaagat
      961 gaactggaga cggagcgcaa gaaggaactg caggcagctc tggacaaata tgcaacggaa
     1021 actcctacac ttccggaaga ggcaattttc attctgagct ctggtgattc gattcgcgga
     1081 aagattctgg agctcgattc ggatacaatc accatcgcag tgcgaagcac agtggagcag
     1141 gcgtcccagg tcgattcggt gaacgcggcc agcattattc tacgagatgg aaaaacggtg
     1201 gtgggtgaag tgatcggcca ggacaatcag agcattagcc tgaaggttgg cggccgcaca
     1261 gtcgtgattc agaaagaaag aatcaagaga atctcttatc agcgagcatc cacggagaag
     1321 gaaggcaaac ctgagactca ggtggaggaa gtggtgcttc cgcgagcaca aataaagcga
     1381 atcgttctga atcgaccacc taacggggca gtgaactaaa gcctggctga aggttgtagt
     1441 gaatgttcta ttcgaatcca aacaactggc gttcaatgat cgcacagaag atctgaccga
     1501 tcatgatgtg gcattcctgg atgcgtgcgg tttctgcggt tggaaccaca atgatatgat
     1561 cagccaggtt tccctctttt gcaatttgac caccgcttcc accagtgagt acaaacgtca
     1621 tcatatgctt cttgcgtgcg gcgtgaagag cttgaatgat atttgctgaa ttgccggaag
     1681 tgcttatggc gagaatcgcg tccccctggc tgcctagacc ttcgatctgc cgcgaaaata
     1741 catactcaaa accaaagtca ttgccggctg ccgtgacagc tgctgcatcc cctacaaggg
     1801 aaattgccgg aagcgatctt ctttcgggag cggaacggta gcggatgagc aattccgtgg
     1861 cgatgtgctg ggcatcgccc gcgcttccac cgtttccaca tgtaaaaaga cgcccgcctt
     1921 tctgcattag ttctgcgagt gcctgccccg cagctgagat tctgtccgtt tctttttcta
     1981 gcagcatctt cttgacatca atactgcgtt ctatatgatc ccggacaagg ttgtgcattg
     2041 gggaagtctt tcgaagcaga aagtctcgtc aatcaattcg gacctgaggg cttatggcag
     2101 aaaatccaag attgattgcg gctctggatt ttattcttaa tcatgctaca ggcgcggaat
     2161 tagaagcgct tgcgtcagca ctttcgcgcc gcgggaattc cagcgcatcg agtctaaatt
     2221 ttcaggacat ggcgagcagc gttacgcgag ggctttccgg aagatttgcg atgccggaga
     2281 acatgaacga agtgacgcgc aacatggtga aaggcatgat tcgccagcat gaaccaaatc
     2341 tttcgccaga acagattgac gtcttgctgg atcaatgggt acccagtccg cagcaagtgc
     2401 gccagggaca agaaggaaaa tatccacccg aggttctcta ctcgatggcg gagcaatttc
     2461 tcatgtatgg tattggcaat atgcctgatt cagaagcaag ggagttgaaa gaagcaatgc
     2521 cggactggca tgagaaatac tggaacatat tcagccccgt gattcgaacg cttctggcgg
     2581 atgcgatccg agggaatctc gggttgagtg aatgccgtgc aaggttgaaa gctcatctgc
     2641 ggctctgaaa gccgcagacg ttaggcgatt ctgtgagatc aggatccctt tttgagcgct
     2701 gcgagcatct gactcgcttt ttctgtttgg gcgtccttga aatctccctt caacttcaag
     2761 aactcttcga gattctcaat ggatttgctc ttgttctttg tcagaaaata caggaagcca
     2821 aggtggtagc gtggagtcgg atcccccggg cgatactgcc gggatttttc aaatacttca
     2881 atggctgaat cgtatttctt ttgatctgtg tagatggcgc ccagcatgct tgagcagaga
     2941 gcgcaatccg gatgcatggc cacatattga ttcagcctgg ctgccgctac atcctgattt
     3001 ccggcgagtc tggttgcatc cacgcttccg atgaaagcat cgcggctgtt tggatctacc
     3061 ttcaaaagcg catcaaacat ttgcgctgcg cgtccgtagt tctttgcgac gagcaaggcg
     3121 cggccaagca aaagtagcgt ttcgccgtca tctgctttta gttcattcgc tttctcaagc
     3181 cactgaattg cgtggagaat gtctccgttt tttgaggaaa gtgcccctgc attgaagtag
     3241 gcttcgggaa atttcggatc cagagagact gctttttcgt attgcgccat ggccgctttt
     3301 ggatcattca ttgcctcaag tacaagacct cgattgctca gagctgcagc ataatcaggt
     3361 ttgattttga gtgcttgatc gaattcagca attgcgtctt gatattgttt atctttatat
     3421 agtatcagtc cgcggttgtt gtaggccttg gccatggacg gatcttttgc gagagccgca
     3481 gcatagtaat caagcgcctc tctggaattg tttttttgct ctgcattgac gccctgcaga
     3541 aacatgtcct gcgcgcttgc acacgcagtt agaagaataa gcgaaaagag tgcggatctc
     3601 actgaacctc cggccttggt cgaggagcga ctgttttgcc tgtctttaga ttttcttttt
     3661 caatttcgag tatgactaca gaaagatttt tctggatttc gtcgaagtcc gccagatctt
     3721 caatgccttt catttctcca attcgcgcaa gctgccgcgc gctgtctgga ctgagctttt
     3781 tactttccag tttctctttg tctgtttgga tttcctggaa gggttcgagt tgcagcgctt
     3841 tcccctgacg ctctacttga acgcttcctg tcaggactgc gattgtgttt cgatctgcgg
     3901 aaaaggagaa agaggttccg cgcactccgg cgagtgcagt gggcgaacga accgtgaact
     3961 cttcatctgc tttcattttc ttcagaccaa gtaggagtcc gcctttctgt acgttgatct
     4021 ctgatttttg cgcgagagca cccactgtaa tccgcgacgc attgagtacg cgaaacgccc
     4081 cgccaccggc aatttgcaga tctacttcgg agtctttttc agtaatgatt acatcgccag
     4141 cgtttacctt ctttcccact gtggccggtt ctgattttcc ctgaatttct acagacgcag
     4201 ttccgcgaac ctgcgaaatg atggcacctt ccggtgcttt ggcgcactgc aagacaaaga
     4261 ggaatgcaaa tacaaggact gtcttcatga tacagtctgt gttcgccatt caaaccagac
     4321 aaggcaattt tttccagcgg acataccatc ctggttccca tttcaattgc atggattgct
     4381 tgcccttcca gggtagattt gttacctgga atttgtgagc agcgatgccc gatgggtaaa
     4441 tgatccagat cagctggatc aaatcattca gaaagtgttc tgcaaagtgc aggtgattct
     4501 caggcacgca gatcaaaata tgcccgtcca ggtagagagg ctcggtccag ggcagcttgt
     4561 gctttcgcac actgcatcgg atgccgcggc tcgagtcatt gtagcaagat tacaggaagg
     4621 acttattgtt ctggaatgtg tccctgaccg caggcttcca gatggcaggg aactgtgtaa
     4681 gccgcgccga ctccaggttc accgggcatt gcgaaaagag c
//
LOCUS       DAORFF010000288         4716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10389_length_4716_cov_3.541515, whole genome shotgun sequence.
ACCESSION   DAORFF010000288 DAORFF010000000
VERSION     DAORFF010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10389_length_4716_cov_3.541515"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="PKK76_11880"
     CDS             <1..1266
                     /locus_tag="PKK76_11880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNL69220.1"
                     /translation="PFQLTTEQDKAILAILKEGTTDHAFAFLLQGDVGSGKTLVALAS
                     MLHYTENRVQCAMLAPTEVLARQHFRTFVDLLGLSGVQVDLLISADSKKSKAQVLDRL
                     ARGETDIVIGTHSLLEPEVVFESLGLVIIDEQHRFGVEQRESLRRKGKNPDVIGMTAT
                     PIPRTLCLTEFADLSLVTLKEKPAGRVPIKTIRLAEDRRPGLYKSIRNHVSNGRQCYI
                     VYPVIEESEKLDVRAATDAYEELRTSIFPDLKVELLHGKMKTEDKDRVFREFRAGRVQ
                     ILVSTTVIEVGVDVANATIMVIEHADRFGISQLHQLRGRVGRGSEESFCILMAGETTE
                     DGEERLNAIVNSDNGFELAEVDLKIRGPGEILGKRQHGVDGLRIASLLEDRKLVEESF
                     AAANEWTDLPPGALEFIKNRFSSAEQSLS"
     gene            1272..2528
                     /locus_tag="PKK76_11885"
     CDS             1272..2528
                     /locus_tag="PKK76_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL69221.1"
                     /translation="MQTIPAPSRDIVSFFRALANIGVKQTMSPPEKKRVVLSNQLFLI
                     AAGVTLSFLPLGLALGTSAIGTVTSLIDTLLLAGCFFLNYRGQYKMARVMGLVVGNSL
                     TFFWVLALGNQMYPELFYFTLAAAPLLFIGPDQKKSLYVLSLLSVVLFFVSHELKAKI
                     APMILIPGDIALLAGYSTGVLSFITLLSILYYFHRGTERVESELDSERKKSDRLLLNI
                     LPEPIADRLKAGERLIADRFDRATVLFADIVNFTPMSGSMPADEVVSILSRIFSAFDM
                     LCEKNGMEKIKTIGDSYMAAAGLPEPDENHMQSAARMALDMQQMILEMARREGMDLSI
                     RIGIHSGPVVAGVIAEKKFIYDLWGDTVNLASRMESHGIPGQIQVSQHIYETLHSTFT
                     FETRGLIEVKGKGPMQTYFLTGSTRD"
     gene            2619..4064
                     /locus_tag="PKK76_11890"
     CDS             2619..4064
                     /locus_tag="PKK76_11890"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL69222.1"
                     /translation="MLFLSLLLGTAFALYFQDRRNTLNRLLALQLILIAIWQYTGFLH
                     ALLSAGPPLVRPLQIVAGVVAAYVFFVLGRHLGRLRAGKWEAAGAAWIFLIFIYYMVR
                     ILIPDSGNAAEFVLVDGRPVYGPTPAYVTYTSTLSAFYGVGIFHILKARRREEDTVMR
                     KRMLLIVLTIGAAAMSGIIFINTFYILNIQMPFPIERVIMVSALLFLGYSVLGERAFS
                     NENLIRLVREKEKAIRDRNRVIESELDLARMIHSRLFPDKPPRIPGYDIQGACISTDK
                     VGGDFYDFYTRLENLGIFIADVSGHGIPAAFIASCTKMAFNYSAGHAENGTQLLKKMD
                     VAIARRAVQSMYVTAVYAEIDYASRQLSYCTAGHGPILLHRRSDNTLQHLKAKGSPLG
                     LMTGKPFEMRTLQLIPGDRLVFFTDGLTESINHERKPYTEERFHEFVRGSAESTAHQV
                     TTNAILEILDYSGDRNLADDTTIVVVDVLEL"
     gene            4078..4653
                     /locus_tag="PKK76_11895"
     CDS             4078..4653
                     /locus_tag="PKK76_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNL69223.1"
                     /translation="MPDTSPTIVLVRHGKPTMRVASRPWQWINSHEVNRRMDAYDQAS
                     LDPHWNGPRAPRIEGHHSISSDLVRAIETAKLIVGEDPREISPLFREVPLPRFRRNVR
                     LPSMALVTMSRLGWFWGFMEGEESRIQTNARVREAADHLEDRVQSHRKVALFSHGFFL
                     WLLSRELGERGWTSEKKGPFSYLETAEFLRL"
BASE COUNT         1210 a         1168 c         1161 g         1177 t
ORIGIN      
        1 cctttccagc ttacgacgga gcaagacaaa gcaatccttg cgattttgaa agaaggaacc
       61 acagatcacg cattcgcctt cctgctgcaa ggtgatgttg gttcaggaaa gactctggtc
      121 gctctggcaa gcatgcttca ctatacagaa aacagagtgc agtgcgctat gctcgctccc
      181 acagaagttc tagcacgcca acattttcgt acgttcgtag atttacttgg actttcagga
      241 gttcaagtgg atttgctcat cagcgcagat tcaaagaaat caaaggctca agtgctcgac
      301 aggttggcgc gcggagaaac agatattgtg attggaacgc acagccttct ggaaccagaa
      361 gttgtgtttg agtccctggg cctggtcatc atagacgaac agcatcgctt cggtgtagaa
      421 cagagagaat ctctacgtcg caagggaaag aatccagacg tcattggaat gaccgcaaca
      481 ccaatccctc gaactctgtg tctgacagaa tttgcggatc tcagcctggt tacgctgaag
      541 gaaaaacctg cgggcagagt gccgatcaag accattcggc tggcagaaga tagacgtcct
      601 ggcctttaca aatccattcg aaatcacgtt tcaaacggca gacagtgtta catcgtttat
      661 ccggtgattg aagaaagcga gaaactggat gtgcgtgctg caacagacgc gtatgaagaa
      721 cttcgcacga gcatttttcc tgacctgaaa gtggagctgc ttcacggcaa aatgaaaaca
      781 gaagataagg atagagtctt tcgcgagttt cgcgccggta gagttcagat tcttgtctcc
      841 actactgtca ttgaagttgg tgtggacgtt gccaacgcga ccatcatggt catagaacac
      901 gcagatcgtt ttggaatttc acaactacat caactcaggg gccgcgttgg aagagggagc
      961 gaagaaagct tctgcatttt aatggccggt gaaacaacag aggacggcga agagaggctc
     1021 aacgcgatcg taaactctga caatggcttt gaacttgcgg aagtggacct caagattcgc
     1081 ggcccgggag aaattttggg aaaacgacag catggcgtag atggcctgag aattgcgagt
     1141 ctgcttgaag atagaaaact agtagaggag tccttcgcag ccgcaaacga gtggacagat
     1201 ctacccccgg gcgcccttga gtttatcaaa aatcggtttt catctgcgga gcaaagtctc
     1261 agctgatctt catgcagacg atccccgctc catccagaga tatcgtcagt ttctttagag
     1321 cccttgcaaa cataggcgtg aagcagacca tgagtccgcc ggaaaagaag cgtgtagtgc
     1381 tcagcaatca gctcttcctg atcgccgcgg gcgtcacttt gtcttttcta cctctgggac
     1441 tggccctggg cacatctgcc attggcactg tgacgagctt gatcgacact ttgcttctgg
     1501 ccgggtgttt ctttctcaac tatcgcggcc agtataaaat ggctcgagtg atgggcctcg
     1561 ttgttggaaa cagtctcacg ttcttctggg ttctcgctct gggcaatcag atgtatccgg
     1621 aactttttta tttcaccctg gcagcggcac ccctgttgtt cattggcccg gatcagaaaa
     1681 aatctctcta cgttctctct ttgctctctg ttgtgctctt ttttgtttcg catgaactca
     1741 aagcgaagat tgcacccatg atcctgattc ctggagatat cgcacttctg gctggctata
     1801 gcaccggtgt tctttctttc attacactgt taagtattct ttactatttt caccgtggca
     1861 cagaacgagt ggaaagcgag ctggattcgg aaagaaagaa gtcagatcgt ttgcttttga
     1921 atattcttcc agaaccaatc gcggatcggc tgaaggcagg agaaagactc attgcagatc
     1981 gatttgatcg agccacagtg ctctttgcag acattgtaaa tttcacacca atgtccggaa
     2041 gcatgcccgc ggatgaagta gtgagtattc tcagcagaat cttctccgca ttcgacatgc
     2101 tttgcgaaaa aaatggaatg gagaaaatca aaacaatcgg tgattcttac atggcggctg
     2161 cgggcttacc cgagccagat gaaaaccata tgcaatccgc agcccgcatg gcactggata
     2221 tgcaacaaat gatcctggag atggcccgca gagaaggaat ggatctaagc attagaatcg
     2281 gaattcattc cggcccggta gtcgccgggg tcattgcaga aaagaaattc atctacgatc
     2341 tgtggggtga caccgttaac ctcgcaagca ggatggaatc ccacggaatt cccgggcaaa
     2401 ttcaggtttc ccagcacata tacgaaactc tgcactctac cttcacattt gaaaccagag
     2461 gcctcatcga ggtgaagggg aaagggccca tgcaaacgta ttttttaacc ggttccaccc
     2521 gcgattgagc aaaattcggg tgactgatct gaaaacctcg tctaatctga gaggatttta
     2581 ttttggtttc ggaatttgcc accagagttg ccctccttgt gctcttccta tccttgctcc
     2641 ttggcacggc attcgcactc tatttccagg acagacgcaa cactctcaat agactactcg
     2701 cactgcaact catcctgatt gcgatctggc aatacaccgg ttttcttcac gcactccttt
     2761 ccgcaggccc gcctctggtg agacctcttc aaattgtagc cggtgttgta gcagcgtatg
     2821 tgttttttgt tctggggagg cacctgggca gactccgagc cggcaaatgg gaagcagcag
     2881 gcgctgcctg gatattctta atatttatat attatatggt tcgtatacta attcctgatt
     2941 ctggaaatgc agcggagttt gtactggtcg acggtaggcc agtgtatgga ccaactccgg
     3001 cctatgtcac ttacacgagc acattgtccg cattttacgg agtaggaatc ttccatattc
     3061 taaaagcaag gagacgcgaa gaagatacgg ttatgcgaaa gagaatgctc ttgattgtac
     3121 taacaatcgg ggccgctgcc atgtccggca tcatctttat taatacgttc tacatactga
     3181 atatccagat gcccttcccc atcgaaagag tgatcatggt aagtgcgctt ctattcctgg
     3241 gttattccgt tctcggagaa agagcattta gcaatgaaaa cctgattcgc ctggtcagag
     3301 aaaaggaaaa ggcgattcga gatagaaatc gtgtgatcga aagtgaactc gatcttgcgc
     3361 gcatgatcca cagtcgtctt tttccggaca agccaccgag gattcctgga tatgacattc
     3421 agggcgcctg catttccacg gataaagtgg gcggggattt ttacgatttc tatactcgcc
     3481 tggaaaatct gggaatcttc attgcggacg tctccggtca tggaatcccc gccgcgttca
     3541 ttgcctcctg cacgaaaatg gctttcaact attctgccgg gcatgcggaa aacgggacgc
     3601 aacttctcaa aaagatggat gtggccatcg cacgccgcgc tgtacaatcg atgtacgtta
     3661 cagctgttta cgccgaaatc gactatgcaa gtcgccagct ttcctattgc acggccggac
     3721 atggaccaat actgcttcat cgcagatccg acaatacact ccaacatttg aaggcaaaag
     3781 gatcgcctct cgggcttatg accggcaaac catttgaaat gcgaaccctc cagcttattc
     3841 caggagatcg ccttgtattt tttacggatg gcctgacaga atctatcaac cacgagcgca
     3901 aaccatatac ggaggaaaga ttccacgaat ttgtgcgtgg atcagcagaa agtaccgcgc
     3961 atcaggtcac tacaaacgca atccttgaaa tcctggacta ttcaggagat cgcaatctcg
     4021 cagatgatac caccatcgtt gtagtcgacg tcctggaact ctgaacaaag ctgacgcatg
     4081 cccgatacgt ctcctacgat cgtgctcgtg cgtcacggaa aacccacgat gcgcgttgct
     4141 tcgcgcccct ggcagtggat caactctcac gaagtcaatc gcagaatgga tgcgtacgac
     4201 caggccagtc tggatcctca ctggaatgga ccccgtgctc cgcgaattga aggtcatcat
     4261 tccatctcct cagaccttgt gcgcgccata gaaacggcca aactgattgt aggagaagat
     4321 ccgcgcgaaa tcagtcctct ctttcgagaa gtacctcttc caagattccg tcgcaatgta
     4381 cgccttccat ccatggcgct tgttaccatg tcacgccttg gatggttctg gggttttatg
     4441 gaaggggaag aatcccgcat ccaaaccaat gcgcgggtgc gcgaggcagc agatcatcta
     4501 gaagatagag ttcaatccca cagaaaagta gcactcttca gccatggctt ctttctatgg
     4561 ttgttatctc gggagctcgg tgagcgcggt tggacttctg aaaaaaaggg ccctttttct
     4621 tatctggaga cggcagaatt cctgcggctg taatttctgc tagagacctt cgaggctacg
     4681 cgggaaaaca agcttcgtgt actttctcag tccacg
//
LOCUS       DAORFF010000289         4709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10410_length_4709_cov_3.926085, whole genome shotgun sequence.
ACCESSION   DAORFF010000289 DAORFF010000000
VERSION     DAORFF010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10410_length_4709_cov_3.926085"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..685)
                     /locus_tag="PKK76_11900"
     CDS             complement(119..685)
                     /locus_tag="PKK76_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL69224.1"
                     /translation="MRIVLAHSYDIVTQGLRAELAAFSSLKLAFLKPLYPRVYAMGTS
                     VGATSILIALPGMAELSAVIAENPMFSFQRFVDETPAAPSFLPGWFKFLVVSIAKKRG
                     LFDGLSSAQNSLRIASTVPIFFIHSKADGLIPWKHSEELHALYNGPKKLWITETGEHA
                     AVWNTHKEEYERALTDFLNQPPIIAPRR"
     gene            769..1539
                     /locus_tag="PKK76_11905"
     CDS             769..1539
                     /locus_tag="PKK76_11905"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNL69225.1"
                     /translation="MNQMAAAESIQAGLNPAFLLLGCIVLFSYTTQAITGFGSTVIAL
                     ALSAHIYSIPQLLPVLVGLNLPLCLYFVIQHRKSIESTLVFKEILPWMSAGVGVGLIL
                     LASIPVPALKPMLGILIVIFAVREGIRLMRRTGSMPAMSNAQFRFWTFASGITHGIYA
                     SGGPLLVYAVSRKNLGASVFRSTMMTIWLFFNFFLIGFYAYEGRWSSQLVEAIFLLLP
                     LIPIGIYLGEFLHHRISVRTFWFVVQSVLLVSGVSFLV"
     gene            1628..3367
                     /locus_tag="PKK76_11910"
     CDS             1628..3367
                     /locus_tag="PKK76_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNL69226.1"
                     /translation="MAELSGGELIARILKQEGVKVVFGIIDGTYFGFYSSLRKNGIQL
                     IGPRHETSALHMAAAYARLTGELGVCMASNGPGVANALPGVAVENVEGNRVLLITSAR
                     RVGIMYPDRGGTYQCFDQTGVIRPMSKWSEHVPNFGRITEMTHTALRMSWTGRPGVVH
                     LDVPEDIINTKVKASPSLLSKNKFRQTEPLHPSEDQVERAAKMLLSAGMPLIHAGSGV
                     IHALAFDELARTAELLDAPVTMSWCARGAAVETTRVFPMIDVAMNNKLRNDADVVLTL
                     GSRLGETDWWGKAPYWRHPSKQKMIQVDIEGRWMGANKPIDLGIQADVKVFLQMLNAK
                     IARARVPSRKTAIDRYNAERNRERAKLAENLKDDSSPMYPGHMAAIARKVFPDDTILV
                     ADGGNTTIWANFFWDVRKPNTILSTFKMGMLGAGVAQALGACVARPKSKVLCIIGDGA
                     MGFHPQEIETAVRNKLKPIYVVACDRQWGMVKMNQQFALKPIKTLIKKSLGPHETINA
                     DLGEIRFDLLAQSMGAHGERVSTPAEFKKALERSLAADKCAVIHVDVNPVKHMWVPGL
                     IYFKDMHKEPKGK"
     gene            3364..4317
                     /locus_tag="PKK76_11915"
     CDS             3364..4317
                     /locus_tag="PKK76_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNL69227.1"
                     /translation="MKRILVTGAAGYIGSLFIAGLAAKKSGAKILGVDVREPLHPVRG
                     TNFEKCDVSSTELRAVFKKFKPDTVVHLASIVSPPKNSDPQLEYRVDVLGTKNVLEGC
                     AETKTKKIIVTSSGAAYGYHSDNPEWLKETDQLRGNDSFPYSRHKRLVEEMLSEYRKK
                     HPSLTQLIFRPGTVLGAGTRNQITNLFERSFVPGVAGSDSPFVFIWDQDVVECLIRGA
                     TTSKTGIYNLAGDGKVDLPEIARILGKKFIPIPAALLQTVFAICKPLGLVRYGPEQVD
                     FLRYRPVLSNKMLKEKFGFIPRKTSREVFQYYIENSPHLRK"
     gene            4319..>4709
                     /locus_tag="PKK76_11920"
     CDS             4319..>4709
                     /locus_tag="PKK76_11920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL69228.1"
                     /translation="MENEGKRSRGQAVFTFVLLFLLYFFDYADRTVVNSLFPFLKKDW
                     GLTDAECGSLTSIVTFTIAAGTLPISVLIDRWSRTRVIGTMAVIWSLFTAACGFAGSF
                     AQLFAARIGIGLGEAGYAPGGTAMIAGL"
BASE COUNT         1231 a         1138 c         1204 g         1136 t
ORIGIN      
        1 cactgtacga gttggacgga caatggaatc gcagtgagtg gaagcgtcgg agatggatcc
       61 tcgacgggtt ccaatgcgat ttccattacc accaacacgt gcgatacgct caagccactc
      121 atctgcgtgg agcaatgatg ggtggctgat tcaagaaatc agtcaatgct cgttcgtact
      181 cttctttgtg agtgttccat acagctgcat gctctcccgt ttccgtgatc cagagttttt
      241 ttggtccatt gtacagagca tgcaattcct ctgaatgttt ccacggaatg agtccatccg
      301 ctttgctgtg aatgaagaag ataggcaccg tgcttgcgat gcgaagactg ttttgtgcgc
      361 tcgacagccc atcaaatagc ccgcgctttt ttgcaatgct tacaaccagg aatttgaacc
      421 atcctggtag aaatgaagga gccgcgggcg tctcatccac gaatctctga aaactgaaca
      481 ttggattttc tgcgatcaca gcactcagct ccgccattcc gggaagcgcg atcagaatag
      541 aagttgctcc cacagatgtt cccattgcat acacgcgagg gtaaagcggc ttcaggaatg
      601 cgagtttgag agaagagaaa gcagcgagct ctgcgcgcaa cccctgagtc actatatcgt
      661 aagaatgggc cagtacaatt cgcacggacc ctgaatctta cggcgtgcac agagtggtca
      721 agcaactgtg gttttcaaca gttgcgcgac tggcatgcaa atggtagaat gaaccagatg
      781 gcagccgctg aaagcattca agccgggcta aatccagcat tcctgcttct gggctgcatc
      841 gttctatttt cctacacaac gcaggcgatc actggatttg gaagcacagt cattgcgctt
      901 gctctgagtg ctcacatcta ctccatccct caacttctgc cagtactcgt tggactcaat
      961 ctcccactct gcctttattt cgtgatccaa catagaaagt ccattgaatc cactctggtc
     1021 tttaaagaga ttttaccctg gatgagcgcg ggagtgggcg tcggcttgat tctacttgcg
     1081 tcgataccag ttccggcgct caaacccatg ctcgggattc tcattgtcat tttcgcagtg
     1141 cgagaaggaa ttcggctcat gcgccgaact ggatccatgc ctgccatgag caacgcccag
     1201 tttcgattct ggacatttgc ctctggaatc acgcatggga tttatgcatc tggaggtccg
     1261 cttcttgtgt atgccgtgag cagaaagaac ctgggcgctt ctgtatttcg aagcaccatg
     1321 atgaccatct ggttgttctt caatttcttt ctaattggtt tttacgcata cgaagggaga
     1381 tggtccagcc aactggtgga agcgatcttc ttgcttttac ctctcattcc gattggtatt
     1441 tatttgggag agtttcttca tcacaggatt tcagttcgca cgttctggtt tgtagtgcag
     1501 tcggtgctcc tggtatccgg ggtgtctttt ttagtgtagg aatagttgaa aatgacaagt
     1561 gaacagacac ccaaaattag ttaagaaaca tatactacag atttgaaaca aaaagcgggg
     1621 gatcaaaatg gcagaactga gcggcgggga actcattgcg agaatcttga aacaggaagg
     1681 cgtgaaggtt gttttcggaa tcattgacgg aacctatttt ggattctatt ccagccttcg
     1741 caaaaacgga atccaactta ttgggcccag gcatgaaacg agcgcacttc atatggcggc
     1801 agcctacgct cgtctgacgg gggaactggg tgtatgtatg gcgtctaacg gtcctggagt
     1861 ggccaatgcg cttcccgggg tagcggttga aaacgtagaa ggcaaccgcg tgctactgat
     1921 tacaagcgcc aggagagttg gcatcatgta tccggaccgc ggtggaactt accagtgctt
     1981 tgatcagaca ggcgtgattc gacctatgtc caagtggagc gagcatgttc caaactttgg
     2041 tagaatcact gaaatgacac acaccgctct tcgcatgtcc tggactggaa gaccgggagt
     2101 cgttcatctg gatgtgccgg aagacatcat caatacgaaa gtaaaagcaa gcccttctct
     2161 gctatccaag aacaagttta gacaaacaga accactgcat ccatcggaag accaggttga
     2221 gcgcgcagcg aaaatgcttc tgtccgcggg tatgcctctc atccacgcag ggagcggtgt
     2281 gattcatgct ctcgcattcg atgaacttgc gcgcacagca gaactcctgg atgcgcctgt
     2341 gaccatgagc tggtgcgcga gaggcgcggc tgtggaaaca acacgcgtat ttcctatgat
     2401 cgacgttgcc atgaacaata aactcagaaa cgacgcagac gtggttttga ctctgggttc
     2461 gcgtctcggg gaaacggact ggtggggaaa agctccttac tggagacacc cttccaaaca
     2521 aaagatgatt caagtggaca tagaaggccg ctggatgggc gcaaacaaac ctatcgatct
     2581 tggtattcag gcagatgtta aagtattttt acaaatgctc aacgcgaaga ttgcgagggc
     2641 acgtgtacct tcgcgcaaga ctgcaattga tcgctacaat gcggaacgca atcgggaaag
     2701 agcaaaactg gcagaaaacc tcaaggacga ttcgtctccc atgtatcccg gacacatggc
     2761 ggccatcgcg cgcaaagtct tcccggatga tacaatcctc gttgcagatg gaggaaacac
     2821 aaccatctgg gccaactttt tctgggacgt gcgaaaacct aatacgattc tctcgacgtt
     2881 taaaatgggg atgctcggag cgggtgttgc ccaggcactg ggcgcatgcg tcgcgcgacc
     2941 aaaatcaaaa gttctctgca tcatcgggga tggcgccatg ggatttcatc cgcaggagat
     3001 agaaacagca gtgcgaaaca aactaaagcc aatctacgtg gtggcctgcg atcgccagtg
     3061 gggaatggtt aagatgaatc aacaattcgc tctgaagcct atcaaaactc tgatcaagaa
     3121 atcgcttgga cctcacgaaa ccatcaacgc tgaccttggc gaaattcgat ttgatcttct
     3181 ggcacagagc atgggtgctc acggagaaag agtttcgacc ccggctgagt tcaagaaagc
     3241 actcgaacgt tcgctggcgg cagacaaatg cgcggtgatt cacgtggatg tgaatccggt
     3301 caaacacatg tgggtgcccg gtctgatcta cttcaaagac atgcacaaag aaccaaaggg
     3361 caaatgaaac gcattctagt aacaggtgcc gcaggctaca ttggttcgct cttcatcgca
     3421 ggcctcgcgg caaaaaagag cggagcaaaa atacttggcg tggacgttcg ggaaccactc
     3481 cacccggtac gcggcacgaa ctttgaaaag tgcgatgtga gttcaacgga gctccgggca
     3541 gtatttaaga agttcaaacc agatactgtg gttcatcttg catccattgt cagtccgcca
     3601 aagaacagcg atccacaact tgagtaccga gtggatgtgc tgggaaccaa aaatgtactg
     3661 gagggatgcg cagagacaaa gacaaaaaag atcattgtta cgtctagcgg ggctgcctac
     3721 ggatatcact cggacaatcc agaatggtta aaggaaacag atcaacttcg cggcaatgat
     3781 tcgtttcctt attccagaca caaacgactc gttgaggaaa tgctctcgga gtaccgcaaa
     3841 aagcatcctt cgctgactca actcatcttc cgtcctggaa ctgtacttgg tgcaggaacg
     3901 cgaaaccaga tcacaaatct atttgaaaga tcttttgtgc ctggagtggc cggttctgac
     3961 tctcctttcg ttttcatctg ggaccaggat gttgtggaat gtttgatccg gggagcaacc
     4021 acgagcaaaa cgggaatcta caatctagcg ggagacggaa aggtagatct gccagaaatc
     4081 gcgcgcattc taggaaaaaa attcatcccg attcctgccg cattgctaca aacagttttc
     4141 gccatctgca aaccacttgg cctcgtgcga tacggccccg aacaggtgga cttcttgcgc
     4201 tacagacccg tgctctcaaa caaaatgcta aaagagaagt tcggttttat tcccagaaaa
     4261 acttcacgcg aagtctttca gtactacata gaaaatagtc cacacctgag gaaatgatat
     4321 ggagaacgag ggcaaacgca gtcgcggtca ggcagttttc acatttgttc ttttgtttct
     4381 attgtatttc tttgactacg cagatagaac cgtagtgaat tctcttttcc ctttcttgaa
     4441 aaaagactgg gggctcacag acgcggaatg cgggtcactc acttctattg taactttcac
     4501 aatcgccgca ggcacacttc ccatttccgt gctcattgat cgctggagta gaaccagagt
     4561 gattggcacg atggctgtga tctggagtct attcactgca gcatgcggat ttgcgggaag
     4621 ctttgctcaa ctctttgctg cgcgaattgg aattggactg ggagaagcag gttacgcccc
     4681 aggtggcact gccatgatcg caggtcttt
//
LOCUS       DAORFF010000290         4705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10420_length_4705_cov_3.486022, whole genome shotgun sequence.
ACCESSION   DAORFF010000290 DAORFF010000000
VERSION     DAORFF010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10420_length_4705_cov_3.486022"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKK76_11925"
     CDS             <1..380
                     /locus_tag="PKK76_11925"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HNL69229.1"
                     /translation="MNPTIEYGVTFERAMQVELDQGRYVYISGTASIDNEGCIVCELD
                     LEGQVNRTVENIAALLESCEMRLEDLMYVCIYLRDFNEEHRTRSCIQKLLPGNVPFVL
                     LHAPVCRPGWLIEIDGLARKTSL"
     gene            483..1742
                     /locus_tag="PKK76_11930"
     CDS             483..1742
                     /locus_tag="PKK76_11930"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL69230.1"
                     /translation="MHNTLEQILRQREVRGERTVALLRAAFISLAVFLDVLAFAHVIP
                     PYTSSPPAVSTLLLDAFFLSAALAFAILALKGVYYGFLKFVTITLDYVLILVMFAFDP
                     SLAGAGIENLWLAVIAPVFMLYLNLIRFSQSAAWYAAGLSVAQCAIVARLILGSWELQ
                     KILPLEFGLLMLISIGLAVSAAGRQMTEEASAKQMLERYLPPQLVKELQRSKASLVPG
                     GRRQFVTMLFADIRGFTRISETLSPEQVVEVLNDYLSTMTDVIFAHEGTIDKFIGDAV
                     MTIFGSPVSRPDDVVRAFRAAQGMILALDEFNARHPELPSPIAIGVGLHAGEVIAGNI
                     GSAKRLDYTVIGDNVNLTSRIEALTKHYGCRILASSAVIEGLKRCGVEPAAREIDTVI
                     VQGKTQSVVVYELFVEAAPATVSSTQF"
     gene            2228..3403
                     /locus_tag="PKK76_11935"
     CDS             2228..3403
                     /locus_tag="PKK76_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69231.1"
                     /translation="MRGATFLQGIAFLFAAGIGCTPALPVSANLDETTALVAGIGLLP
                     RSTTASATVITRVMVVLGNNTTTTNIYDPSTNMFSNGPTLTGSAAPGTIQFTIPSGTH
                     SGKTLIVHGASSSMYDPATGSMVAGPAPSGTLGIGSAAFVVPAGGAQPGKVMIVHGGS
                     LLTTSLYNPATHTMGAGAALGFGPTGGSFTMYIPTGTQGGKFMTMQAGAQRRAMFYDP
                     VGDSYATSANQTTANVGGDAWAYLMTSGANPNKIFVVVANGASTTNLYDLASDTWAAS
                     SPVLTAAASPGSNGFLVPSGGNSGKLLVLHGALTSSSFYDPSGNTMGGGPALTGNMGG
                     GSCVFSITAGPQMGKVMVVLGGNQTVTNIFDPSASTFSAGPSLTANANGGAQCFSIQ"
     gene            complement(3464..4117)
                     /locus_tag="PKK76_11940"
     CDS             complement(3464..4117)
                     /locus_tag="PKK76_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69232.1"
                     /translation="MHYKIVDDALIESPYKFREKRLVLFSFLVLLLIAACSERTVFEY
                     RDKNESCCKFGVPAGRTGADVTVHLITDRAGMFESAYPSLVLEYVTSVQDAGSVRKID
                     VNLATEHELVPTLFEIEAGYYDAFTTQKFPNSIPVSECCQGIHLKIRNEASTYQGDFG
                     PVHFGIRIRKEYASIRSLPPEMNLIVTVETDKGVFKDARAVHLISYKSKGGVPFRFH"
     gene            complement(4083..>4705)
                     /locus_tag="PKK76_11945"
     CDS             complement(4083..>4705)
                     /locus_tag="PKK76_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69233.1"
                     /translation="RRDEPVIWSDATVDPGPPFPYHKLTNEKIIENPFILCDSGYLLD
                     LRPIASTDFAFPNSLIQNAEPVLSARVAIEGSALYAEVKTGRGTKTYSLNVKAAKDSD
                     GLTALRETPQVPASLQWKEVDIIELPAKERTFVFSKRGPMFVSVEPRDFRGKFLFIQS
                     YYQRSRHIYEMWPALLYPLAIAVDLVTSPIQIPLLLALQDCGRCFD"
BASE COUNT         1061 a         1194 c         1256 g         1194 t
ORIGIN      
        1 acatgaaccc cacaattgag tacggtgtta cttttgaacg tgcgatgcag gtggaactgg
       61 atcagggacg ttatgtttac atttccggca ctgcgagcat tgacaatgag ggttgcattg
      121 tttgcgaatt ggatctggaa ggacaggtga atcgaaccgt tgagaatatc gcggcgctgc
      181 ttgagtcttg cgaaatgagg ctggaagact tgatgtatgt atgcatctac ttgcgggact
      241 tcaatgaaga gcacaggaca agatcctgca ttcagaaact cttgcctggt aatgttccgt
      301 ttgttttgtt gcacgcacct gtttgccgtc ctggctggct catcgaaata gacggtctgg
      361 caagaaaaac aagtctttga ctttgccgcc ttctgccttc ttccgcgcgc ggtgagaact
      421 ttctctgctt cgtctggaaa aggatggcct gcctaccggc caccggataa gagagcgtgt
      481 ccatgcacaa tactctggaa caaatcctgc ggcagagaga agttcgcggg gaaagaaccg
      541 tagctctcct gcgggctgcg ttcatctcac ttgccgtctt tctcgatgtc cttgcattcg
      601 ctcacgtcat ccctccctat accagttcgc ctcccgcagt gagcactctg ttgctcgatg
      661 cgttttttct ttcggcagct ctggcttttg cgatcctcgc actgaaggga gtttactatg
      721 gcttcttaaa gtttgttacc atcacccttg attatgttct gattcttgtg atgttcgcgt
      781 ttgacccgtc gcttgctgga gcagggatag agaacctgtg gcttgctgtg attgcacctg
      841 tgttcatgtt gtatctgaac ctgatccgct tctcacaatc ggcggcctgg tatgcggcgg
      901 gtttgtctgt cgcacaatgc gcgatcgttg cgcggttgat cctcggttcc tgggaactgc
      961 aaaagattct tccgcttgag ttcgggctct tgatgctcat ttccattgga cttgctgtaa
     1021 gcgccgcagg ccgccagatg acggaagaag ccagtgcaaa gcaaatgctt gaacgttatc
     1081 tgccaccgca gctggtaaaa gaattgcagc gaagcaaagc aagtctggtt cccggaggca
     1141 ggcggcagtt tgtcacgatg ctgtttgcag atatccgcgg cttcaccagg atttcagaga
     1201 cgctaagtcc ggagcaggtg gttgaggttc tcaacgacta tttgtccacc atgacagatg
     1261 tgatttttgc tcacgagggg accattgata agttcatcgg cgatgcagtc atgacgattt
     1321 tcggatcgcc cgttagccgg ccagacgacg tggtgcgtgc ctttcgcgcc gctcagggaa
     1381 tgattctagc gctcgacgaa tttaacgctc ggcatccaga gcttccgtcg cccattgcca
     1441 ttggtgtggg tctgcatgca ggagaggtca ttgccggcaa cattggctct gcaaagcgac
     1501 tcgactacac cgtgattgga gacaacgtca atcttacttc acgcatcgag gcgctgacga
     1561 agcactatgg ttgcagaatc ctggcaagca gtgctgtgat agagggactc aaacgctgcg
     1621 gtgtggaacc ggccgcgcga gaaattgata cggtcattgt gcagggtaaa acgcagtctg
     1681 tggtggtgta cgagctgttt gtagaggctg ctccggcgac tgtttcttcc acacagtttt
     1741 aaaaccgctg cccacgtgcg tccaggtcat ctggttgccc tgaatgatca ggcggtaacg
     1801 aaaatctgtg ccgattccgc aagaatgcgt tgtatctgat atgatgtgta agatgtcgcc
     1861 ttctacagag taagaaaaag caaagcagag gtcttcgttt cgaagataag ttccgcgccc
     1921 accatcgccg aacaaaactg catcccctgt ccaggtccat ctgcccttga gagtgttttc
     1981 tcctgcaatg cgaccgaccg atgtctggca ggcacagagg cctgccaaaa agattaaagc
     2041 gaccagccgt cgaggggccc gcgcgagttt cacaatggag aaagagctga tatgcacgcg
     2101 actgcaaacc atattttgct ttgactcacg tttctacagc agcgaaagat cgtctaacgt
     2161 acccaaaaat ttcaagatgg gtttcggaaa tagattgaaa gagcccgagc catacgtcta
     2221 ctgagatatg cgtggagcca ccttcctgca ggggattgcc tttctgtttg cagccgggat
     2281 cggctgcact ccagctttgc ccgtttccgc taatctggat gagaccactg ctcttgtggc
     2341 tggaattggc cttcttcctc gttctaccac tgccagtgcc acggtaatca cgcgggtcat
     2401 ggtggttctt ggaaacaaca caacgaccac aaacatctac gacccttcta cgaacatgtt
     2461 tagcaacggt cccacactca ccggaagtgc agcacctggg acaattcagt ttacgattcc
     2521 ttccgggact cattcgggca agacgttgat tgtgcatggc gcatcctcct ccatgtatga
     2581 tccggctact ggttccatgg tggcggggcc cgccccttcc gggaccctgg gaattgggtc
     2641 ggctgctttt gtagtgcccg caggtggtgc gcagccgggg aaagtcatga tcgttcatgg
     2701 tgggagtcta ctcaccacaa gcctctacaa tccggccact cacaccatgg gggcaggcgc
     2761 ggctcttggg tttggcccaa cagggggatc tttcacaatg tacattccaa ccggcacaca
     2821 gggcgggaag ttcatgacca tgcaggccgg tgcgcagcgt cgtgccatgt tctatgaccc
     2881 ggtcggggac tcctatgcca caagtgcaaa ccagaccaca gcaaatgtgg ggggagatgc
     2941 ctgggcatat ctcatgacat ccggtgccaa cccaaacaag atcttcgtcg tcgttgccaa
     3001 cggtgcctct acgacaaatc tatacgatct ggcatcggat acctgggcgg cctcttctcc
     3061 tgttttgacc gccgctgcaa gtcccggatc aaatggtttt ctagttccat cgggaggaaa
     3121 ctctggcaag ctactcgtgt tgcatggtgc gcttacaagc agttctttct atgatccttc
     3181 cggaaacaca atgggtggag gtcctgcact caccgggaac atggggggag gatcctgtgt
     3241 tttttctatc acggctggcc cgcagatggg taaggtgatg gtggtgctcg gcggcaatca
     3301 gaccgtaaca aatatttttg atccttccgc atccactttc agcgcggggc cttctctcac
     3361 cgccaatgca aacggtggcg cccagtgttt ctctattcag tagtacgatc gtggatcgcg
     3421 tgctgtgcat gctgaatagc ggttgcatgc tcacgcaaac gattcagtga aatcggaaag
     3481 gaactccgcc tttcgatttg tagctgatga gatgcacggc ccttgcgtcc ttgaaaaccc
     3541 ctttgtccgt ttcaacggtg acgatcaaat tcatttcggg tggaagagag cgaatgcttg
     3601 catactcttt gcgaattcta attccgaaat gaaccggccc gaaatctccc tgataggtac
     3661 ttgcttcatt tcgaatcttc aaatggattc cctggcaaca ttcggataca gggatagagt
     3721 tcggaaattt ttgagtggtg aatgcgtcgt agtagcccgc ttctatttcg aataacgttg
     3781 ggaccagttc atgttctgtt gcgagattta catcgatctt acggacgctg cccgcgtcct
     3841 gtacagaagt cacatactct agcacgagag aaggataggc cgattcgaac attccggctc
     3901 tgtctgtgat caagtggacc gtgacgtctg cgccggttct tccggcgggc acgccgaatt
     3961 tacaacaaga ttcattcttg tctctgtatt caaatactgt gcgctcagaa caggccgcga
     4021 tcagaagaag caccaggaaa gagaaaagta ccaggcgctt ctcgcgaaac ttgtaaggtg
     4081 attcaatcaa agcatcgtcc acaatcttgt agtgcaagaa gaagcgggat ctgaatagga
     4141 gatgtaacga ggtccactgc aatcgccagg ggatagagaa gtgcaggcca catttcgtaa
     4201 atgtgtctgc ttctttgata gtaagattgt ataaacaaaa actttccgcg aaaatcccgg
     4261 ggctcaaccg aaacgaacat gggccctcgt tttgaaaata caaacgttct ttcctttgca
     4321 ggcaattcga tgatgtcgac ttctttccac tgcagagagg ccggaacttg aggagtttct
     4381 ctgagggcag tgaggccatc cgagtccttt gcagccttca cattcagtga atatgttttc
     4441 gtgcccctcc cggtttttac ttctgcatac agagcacttc cttcgatcgc gacgcgagca
     4501 gacaatacag gttccgcatt ctggatcaga gaattgggga atgcaaagtc cgtggatgca
     4561 atgggtcgca gatccaggag atagcctgag tcgcagagga tgaacggatt ctctatgatc
     4621 ttttcgtttg taagtttgtg atacggaaag ggcggcccgg ggtccactgt ggcatcgctc
     4681 cagatgacag gctcatccct ccgcg
//
LOCUS       DAORFF010000291         4702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10427_length_4702_cov_3.366043, whole genome shotgun sequence.
ACCESSION   DAORFF010000291 DAORFF010000000
VERSION     DAORFF010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10427_length_4702_cov_3.366043"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PKK76_11950"
     CDS             <1..1300
                     /locus_tag="PKK76_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNL69234.1"
                     /translation="LLVIDEEQRFGVAHKEALKKMKVLVDVLTLSATPIPRTLHMSLA
                     GIRDLSIIETPPRNRLPVMTHVMEDSDSQFKEAVTRELERGGQVFYLHNRIDTIERAA
                     ARVRSLIANVQVGILHGRMEDDEVEDILMDFVDNKYQVLVTTTIVENGIDIANANTLI
                     VDDADHFGLSQLYQLRGRVGRSDRQAYAYFFFREKRALSETAMKRLNTILEYQELGSG
                     FKVAMRDLEIRGAGNILGKEQSGDIMDVGFEMYIKLLEDAVARIRGEEHRIDVHTGIN
                     LNQDMYLPESYIPDTRQRIEFYKRFEAADSEEEIDEVLALMQDRFGEAPAEVTTFVAT
                     ERIRVLGSLAGFETIYQEDSGRVQLKAGSRFRVPPTKLLEALQKRIGFQVQAGNPNVL
                     YFEAKGDFFKAFVEALRGIAPPPVVVPEAPGPKNLRGVRS"
     gene            1394..2116
                     /locus_tag="PKK76_11955"
     CDS             1394..2116
                     /locus_tag="PKK76_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69235.1"
                     /translation="MLQRILMLLILTLPLACKDGETIEDIGSYSITTKDFENHYSVLV
                     EKVAQLRNGDKSTFARLMCHPERMPDDPYLREVVRNLEPKEFYERYREMRIIEQAARK
                     ENFQDDPIVKRMMEQAQLEVLTSLFVMKKLEKRIKISDEESAAKCVELRQKLPDQLGP
                     LTLDECKKIAQGFIREERMRTEMPRVREEIKESVTISKNQKFDRDDFLANKLETYKVL
                     KKTGGCDADSAGPALETPAKPK"
     gene            complement(2124..3248)
                     /locus_tag="PKK76_11960"
     CDS             complement(2124..3248)
                     /locus_tag="PKK76_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNL69236.1"
                     /translation="MKGTLYFDYNATHPPIQEILKECLEIYVSNPANSSGISALSQEN
                     QRRIESSREQIAGSLSTKSTQKILPSNLTFVSTGTEALHQMVRTYARPQTRALLSPYE
                     HEAMIAACKEANLSIDMIRADKSGVVDPDEVNRLITPEHSFVSVLAVSNETGAIQPVS
                     SISEITRRKGIPFLCDCIQAAGKLEIDYALFDAFCVNGHKFGAGFGTAVLASRKEPVA
                     LFGGGLQEDERRAGTENLFSILCTASALEMQTALLAEKNERLRRFQKKIEESILEKTS
                     GELTAAGSPRATNTSYFVFPELENIDFLFLGLDQKKVVVSTGSSCKSRTRQPSRSLQA
                     MGYSKQDSMRAVRISTGLFTTEQEVDEFLAAFFYSIQAAS"
     gene            complement(3245..4447)
                     /locus_tag="PKK76_11965"
     CDS             complement(3245..4447)
                     /locus_tag="PKK76_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69237.1"
                     /translation="MLKLLARQIARHWKGLLPVLLLWIAGGYYIFHNKHSILTSLAHF
                     AVKEPAAGEQFPEKARPFVEHAREETHGIRLDLMEKACVQIPLRYRGESELFQPHWLE
                     RYEKWNLQTNREIPPRTDDVVEPDSYWKENRPHVIRALKDTLAASVFAFEIPPAEGSK
                     EGINVADETAKYARAACMDPIIVFMYGDYAEHLEARARLKIVKADKDFELNHPSMQDQ
                     ELYILESLKNSDPYKRALASYMGGPVPITEKACTGREYRLACVAPEEAAIVMRKRIYV
                     ADSAELPALFINLAKLHMLGAGEDHKARALDYFYGGIREISTEKEARAGLARAYMALD
                     KVDRAYEQIAQASLLYRDHGRTDNEFRELARSVLMKAGKFREADCYSDYSDLPSGERD
                     YCVGFKLQ"
BASE COUNT         1171 a         1130 c         1228 g         1173 t
ORIGIN      
        1 acttctggta atcgacgaag aacaacgctt tggtgtggcc cacaaagaag cgctcaagaa
       61 gatgaaagtg ctcgtggatg tgctgactct atctgccact ccgattccgc ggactctcca
      121 catgtctctc gcaggcattc gagatttatc catcattgaa actcctccga ggaatcgtct
      181 tccagtgatg actcatgtga tggaagattc ggattcgcag ttcaaggagg ctgtaacgcg
      241 tgagctcgaa cgtggaggcc aggttttcta tctgcacaat cgaatcgaca cgattgaaag
      301 ggcagcagcg cgcgtgcgct ctttgattgc gaatgttcag gtggggatcc ttcacggaag
      361 gatggaagac gatgaagtag aagacatcct gatggatttc gtggacaaca agtatcaggt
      421 tctggtcaca acgaccatcg tagaaaatgg aattgatatc gcaaacgcga acactctgat
      481 cgtggatgat gcggatcact ttgggctttc gcagctctac cagctccgcg gcagggtagg
      541 cagaagcgac aggcaggcgt atgcttattt ctttttcagg gagaaacggg cgctttctga
      601 aactgcgatg aaacgcctca atacgattct agaatatcag gaactgggaa gcggattcaa
      661 agtcgccatg agagacctgg agattcgtgg agcagggaac attctgggaa aagagcaatc
      721 gggcgatatc atggatgttg gcttcgaaat gtacatcaag cttctcgagg acgcggtggc
      781 gcgcatccgc ggggaagaac acagaatcga tgtgcacact ggaatcaatc tgaaccagga
      841 tatgtatctt ccggaatcgt acatcccgga taccaggcag agaattgaat tctacaagag
      901 attcgaagct gcagacagcg aagaggaaat agacgaagtg ctggctctca tgcaagacag
      961 atttggcgaa gcgccagcag aagtgactac atttgttgct accgagcgca ttcgtgtcct
     1021 cggcagtctg gcaggattcg agaccatcta ccaggaagat tcaggccgcg tccagctgaa
     1081 ggcaggctcc agattccggg tcccacccac gaaattactg gaagcgttgc aaaagagaat
     1141 tggatttcaa gtccaggcag gcaatcccaa cgtgctttat ttcgaggcga agggtgactt
     1201 ctttaaagcg tttgtagagg cgttacgcgg cattgctccg ccaccggttg ttgtccccga
     1261 agctcctggc ccaaagaatc tgcgcggcgt gcgcagctag actgactggc ccggttttca
     1321 tttcagactt gccgcgtgcg ttcgaaactc gattctgccc gcagatcagg gattcgcctg
     1381 acaggagaca ttcatgttgc aacgcattct tatgctgttg atccttactc tgccgctggc
     1441 ctgtaaagat ggagagacca tcgaagatat cggcagctac tccatcacca cgaaggattt
     1501 cgagaaccat tattccgttc tcgtggaaaa agtggctcag cttcgcaatg gagacaaaag
     1561 cactttcgcg cgtttgatgt gccatccaga aagaatgccg gatgatccat atctgcgtga
     1621 agtggttcgc aacctcgaac caaaggaatt ctacgaacgc taccgcgaaa tgagaatcat
     1681 tgagcaggcg gcacgcaaag aaaacttcca ggatgatcca atcgtcaagc gtatgatgga
     1741 acaggcacag ctagaagtgc tgaccagcct ctttgtaatg aagaaactgg agaagagaat
     1801 caagatttca gacgaagagt ccgcggccaa atgcgtggaa ctccgtcaga agcttccaga
     1861 ccagcttggt cctctcacac tcgacgaatg caaaaagatc gcacaaggtt ttatccgcga
     1921 agagagaatg cgcactgaaa tgcctcgcgt tcgcgaagag attaaagaaa gcgtaactat
     1981 ttctaaaaac cagaaatttg atcgcgatga tttcctcgca aacaagctgg aaacatacaa
     2041 agtgctcaag aagactggag gctgcgacgc agatagcgca ggtccggccc tcgaaactcc
     2101 agcaaaacca aagtaaaact ctcttaactg gcggcctgaa tcgaatagaa gaaggctgcc
     2161 aggaattcat ccacttcctg ttccgttgta aaaagccccg tactgatgcg gactgctcgc
     2221 atggaatcct gtttgctata tcccattgcc tgtaaactgc gcgaaggttg tctggttctg
     2281 gatttacagg agcttccagt ggataccacg actttcttct gatccagtcc aagaaagaga
     2341 aagtcaatgt tctctagctc cggaaataca aagtaacttg tgtttgtagc gcgaggcgat
     2401 cctgcggccg tgagctctcc cgatgttttc tccagaatcg attcttctat cttcttctgg
     2461 aatcggcgaa gcctttcatt cttttctgca agtagagctg tttgcatttc aagtgcgctc
     2521 gctgtgcaca ggatagagaa aagattttca gtacctgcgc gcctttcatc ttcctggagt
     2581 ccgccaccaa agagagcgac cggttctttt ctggacgcga ggaccgcggt gccaaacccc
     2641 gctccgaatt tgtggccatt gacgcagaaa gcgtcaaaca acgcgtagtc gatctcaagc
     2701 ttccctgctg cctggatgca gtcgcagaga aaagggattc ctttgcgtcg cgtgatttca
     2761 gagatgctcg atacgggctg gatggcacct gtttcgttag acaccgcaag aactgatacg
     2821 aaggagtgct cgggtgtgat gagtcgattc acttcatccg gatccactac accggactta
     2881 tccgcgcgaa tcatatcaat ggaaagattg gcctctttgc acgctgcaat cattgcttcg
     2941 tgctcgtaag gagatagaag tgcgcgagtc tgcggtcgcg cgtaggtgcg aaccatttga
     3001 tggagcgctt ctgttccggt ggatacaaac gtcagattgg acggcaggat tttttgcgtg
     3061 gattttgtgg agagagaacc tgcgatttgc tcgcgcgatg attctattct tcgctgattt
     3121 tcctgagaaa gtgctgaaat tccgctggaa ttggctggat ttgagacata gatttccagg
     3181 cattctttga gaatttcctg aatgggcggg tgtgttgcat tgtagtcaaa gtaaagagtg
     3241 cctttcattg gagtttgaat ccgacacaat agtctctttc accgctgggt agatccgaat
     3301 agtctgaata gcaatctgct tcgcgaaatt ttccagcctt catcaacacg gacctggcaa
     3361 gttcgcgaaa ctcattgtct gttctaccgt ggtctcgata cagaagcgat gcctgtgcga
     3421 tttgttcgta cgcgcgatct actttgtcga gcgccatata agctcttgct aaacctgcgc
     3481 gcgcttcttt ctctgttgag atttctctaa tccctccata gaaataatcg agtgcccggg
     3541 ccttgtggtc ttctcctgca ccgagcatgt gaagttttgc aagattgatg aagagggcag
     3601 gcaattccgc ggagtccgct acatagattc tctttcgcat cacaatggct gcttcctctg
     3661 gcgctacaca ggcgagcctg tattctcttc cggtgcatgc tttctctgtg attggtacgg
     3721 ggccgcccat gtaggatgcg agcgctcttt tatatggatc gctgtttttt aacgattcta
     3781 gaatgtagag ttcctggtct tgcatggaag gatggttgag ttcgaagtcc ttgtctgctt
     3841 tgacgatctt caggcgagca cgtgcttcta gatgttctgc gtaatcgcca tacataaaaa
     3901 cgatgattgg atccatgcag gcggcgcgcg catactttgc tgtttcatct gctacgttga
     3961 ttccctcttt tgatccttct gcaggaggaa tctcaaatgc gaacacggat gcggccagag
     4021 tgtctttgag cgcgcgaatg acgtgcggcc tattttcttt ccagtaactg tccggttcta
     4081 ccacatcatc cgtgcgaggt ggaatttcgc gattcgtttg caggttccac ttctcatatc
     4141 tttcgagcca gtgcggttga aatagttccg actccccgcg gtatcgcaaa gggatctgga
     4201 cgcatgcttt ctccatcaag tccaggcgaa tgccgtgggt ctcttcccgg gcgtgctcca
     4261 caaagggtct agctttttct ggaaactgtt cccccgcagc cggctctttg actgcgaaat
     4321 gggcgaggga tgtcaaaata gagtgcttat tgtggaatat gtagtagccg cctgcgatcc
     4381 acagcaagag aaccggaagc agtcctttcc agtgtcgcgc gatttgtctg gcgagtagtt
     4441 tgagcactgg agttccttaa aaaaaatgat tggaagcgag gccgattcta gaaatacttt
     4501 ccagcaatgg cacattccaa gaccaaaaat gtaaataaaa aacatcgcaa aaacatccgc
     4561 cgcatgaagg aaaagagagc ggcttctctc ctgaagaaaa agaagaaggg ttgaatttga
     4621 aacgcgctct gctcgtcggc ctggtcttcg catcgggtct ggcgagtcag actatgcccg
     4681 tgatcccgcc gcagccggat tt
//
LOCUS       DAORFF010000292         4674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10527_length_4674_cov_3.315436, whole genome shotgun sequence.
ACCESSION   DAORFF010000292 DAORFF010000000
VERSION     DAORFF010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10527_length_4674_cov_3.315436"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PKK76_11970"
     CDS             complement(<1..681)
                     /locus_tag="PKK76_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HNL69238.1"
                     /translation="MERKDTIKMYKLPVEVKFCRKCTVSNQRPRITFDENGVCSACNF
                     AEYKKTKIDWAQREKELKELCDRFRRNDGGYDVIVPGSGGKDGGFVAHQLKYKYGMNP
                     LTVTWAPLKATEIGRKNLDAFIASGFDNILGTPNGKTTRRLTELAFRYLGDPFQPFIY
                     GQTNFPMHMAVNHGIQLIMYGENGEVEYGGDMKNAHRPTRDIQDHDTHYFSGMPPEFW
                     TEHGVSLAD"
     gene            complement(692..1606)
                     /locus_tag="PKK76_11975"
     CDS             complement(692..1606)
                     /locus_tag="PKK76_11975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HNL69239.1"
                     /translation="MGTAATAAIATLKKNGFAKLSQKVLSSEETKEFESIIDRLHADK
                     SISVDYSANSPSAASLIGRDQRLDFLLNKILAHPEVQGVLSQVVGDDFKIWEISTRIS
                     MDTDPGLGMHVDAVGQCNLAYLINDQTTPAGVTSFWPGSHLLPRSFINKVGLALDWIG
                     SYYWKKMFVPLTGTAGDVAFFVNKTYHGRLPAPASGGQRKIILMGCFPVGSRFKPIQQ
                     HVPFDSIEQPELRKRLSLSQGVHTMGDGFVMVGPEGAKSSSYTMSIEPAGPASFKTFW
                     MASKARLANLCFGVYKRLRPNRSEVSHA"
     gene            complement(1634..3442)
                     /locus_tag="PKK76_11980"
     CDS             complement(1634..3442)
                     /locus_tag="PKK76_11980"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNL69240.1"
                     /translation="MIIIGIYDDHNASAALYKDGEIVFAAQEERFTGRKNEEAFPRYT
                     IEHILKTYNIRPEDIDELAIAGVHHDYVDLMMRKHSVFTIRDYIEEMEKYWKPKLSGQ
                     EFDKDYNLNLFKTQKRFQHQDDVYEYPESLLKLSGVEQLEEFNRIRKRCISKFLGIPE
                     DRVRFYDHHSCHIYYGYYSNPRKSNPTIGFTIDAFGDNRNQTVWKIEGDKFQLLADTK
                     ECELARVYRTVTLYLGMRPYEHEYKVMGLAPYAKEEHAERVADELSEIAQVNGMRIVH
                     KNRPRDLFQFMKERFQYYRFDNVAAGVQMWLEKTATELLTNIHRETGIRNYVFSGGVA
                     MNVKLNKILAEQDFVDDFFVCGSSADESLSIGVAYMAARAREAACKPLHHLYLGPPVD
                     QNGVTEFLKEDAVQSRYEVRRGVTNKDVAKILADGHVVARVAGRMEFGARALGNRSIL
                     ADPGKEDVVKEINEMIKKRDFWMPFALSVLDSFADKYIHNPKGLQARFMANAFDTKPE
                     HYERFRAGTHPYDRSVRPQVVTKDANPQYYSLIEEFAALTGTGALLNTSLNLHGLPLV
                     NDHRQAFHVLDNSGLDFLLIEDVLLVRKKTGETQRT"
     gene            complement(3446..4606)
                     /locus_tag="PKK76_11985"
     CDS             complement(3446..4606)
                     /locus_tag="PKK76_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69241.1"
                     /translation="MRYVALAFYLAGRLRRVLRNARFRSPIQSKLAIFDREGSQYLLG
                     YCLPDVKACVLPVRGEEYLLGARVLWYMLLEVRIFFLRTRFYRDIASRSFHVYLTACI
                     RASGARVVVTHIDNNHHFRWIAEFSGAATFCAVQNGTRLPVEERWNTGEPILPLPEFF
                     CYSQFDIDIFRRYEVPVKQFHPVGSLIAGYYRKRVPWSGSSREFDVCFISDWYPGVHR
                     DFEIPELKRAILVLHYFLRRWTRNSKARVCVATRSPNPGEKEYYKRLLGEATHLADFD
                     PVSRSTYRIMDQSLVAVTSASTAGREAFGWGQRVLFSNFSGLAQYDPPTTGPWHINRA
                     DYSSFARALDDLLNMSDMDYEKQSGPARAYFMPYPDVPAHEFIRNAILTKLE"
BASE COUNT         1178 a         1233 c         1095 g         1168 t
ORIGIN      
        1 atcggcaaga ctcactccat gctccgtcca gaattctggc ggcatgccgg aaaaataatg
       61 agtgtcatga tcctgaatat cgcgtgttgg cctgtgagcg tttttcatat cgccgccgta
      121 ttctacttcg ccgttttctc cgtacatgat cagctgaatg ccatgattca cagccatgtg
      181 catcgggaag ttcgtttgtc catagataaa gggctggaat ggatccccga gatatctaaa
      241 cgccagctca gtcaggcgac gagtcgtctt cccattcgga gtgcccagta tattgtcgaa
      301 accggatgca atgaatgcgt ccagattctt gcgcccgatt tcagtggcct tcagaggcgc
      361 ccacgtcacg gtaagcgggt tcattccata tttgtatttg agctgatgag caacgaaacc
      421 gccgtctttg cccccactgc caggcacgat cacatcgtat ccaccgtcgt tgcgacgaaa
      481 gcggtcgcac agttccttca gttctttttc gcgctgcgcc caatcaatct ttgtcttttt
      541 gtattcggca aagttgcacg cggagcagac gccgttttcg tcgaacgtga tccgcgggcg
      601 ctgattggat acggtacatt tccggcagaa tttgacttcc accggaagtt tatacatctt
      661 gatcgtatct tttctttcca tttttttcct gtcaggcgtg actgacttct gatctattgg
      721 ggcgcagcct tttgtaaacg ccaaagcaca ggtttgccag gcgcgctttg gaggccatcc
      781 agaatgtttt gaagcttgca gggcctgctg gttcaatgct catcgtgtag gaggagcttt
      841 tcgcgccctc tggaccaacc ataacaaatc cgtcacccat tgtatggaca ccctgcgaaa
      901 gagaaaggcg cttgcgcaac tctggttgct caatagaatc aaacggaacg tgctgctgga
      961 taggcttgaa gcgcgagccg acaggaaagc atcccatgag aatgatcttt ctttgaccac
     1021 ctgacgcggg ggcaggaaga cgaccgtggt acgtcttgtt cacaaagaac gccacatcac
     1081 cggcagtacc cgtgagtggg acgaacatct tcttccaata gtaagagccg atccaatcca
     1141 gcgccagacc caccttgttg atgaaagacc gcggaaggag atgcgatcct ggccaaaaag
     1201 aagtcactcc cgccggtgtg gtctgatcat tgatcaaata cgccaggtta cactgcccca
     1261 ctgcatccac gtgcattccc agtccgggat ctgtgtccat ggagatcctt gttgaaattt
     1321 cccagatctt gaaatcatca ccgacaactt gagaaagcac tccctgcact tcagggtgcg
     1381 ccagaatttt gttcagcaag aaatccagac gttggtcgcg accaataagc gatgcggcgc
     1441 tcggactatt tgcagaatag tcaacgctga tcgatttgtc cgcgtgcaag cgatcaatga
     1501 tggattcaaa ctcttttgtt tcctcactcg agagcacctt ctgcgacaat ttcgcaaacc
     1561 catttttctt cagcgttgcg attgcggcgg ttgctgctgt tcccataaat tcctccagac
     1621 tcagcgtgtc gttttaagta cgctgcgttt caccagtttt ctttcgcaca agtagaacgt
     1681 cttcaatcaa aagaaaatca agacccgaat tatcaagcac atgaaatgcc tgcctgtgat
     1741 cattcacaag aggcagaccg tggagattga gactcgtatt cagaagtgct ccggttccgg
     1801 tgagcgcggc aaattcctct ataagactat agtattgagg atttgcatct tttgtcacta
     1861 cctgcggccg aacactgcga tcataaggat gagtccctgc gcgaaagcgt tcgtaatgtt
     1921 ctggtttcgt atcaaacgca ttcgccatga atcttgcctg aagacccttt ggattgtgaa
     1981 tgtatttgtc tgcgaaagaa tcgagcacag aaagagcgaa aggcatccag aagtcgcgct
     2041 tcttaatcat ctcatttatt tctttgacaa cgtcttcctt tccgggatct gccaggatac
     2101 tgcgattccc gagcgctctg gctccgaact ccattctacc agcgacgcga gcgaccacat
     2161 gtccgtctgc gagaatcttc gccacatcct tgttcgtaac accccgacgc acttcgtagc
     2221 gactttgcac cgcgtcctct ttcagaaact cggttacgcc attttgatct acgggcggcc
     2281 ccagataaag atgatgcaga ggtttgcagg ctgcttcgcg agcgcgcgcc gccatgtatg
     2341 caacaccgat cgagagcgat tcatccgcgg aagaaccgca aacaaagaaa tcatccacga
     2401 agtcttgttc cgcgagaatt ttgttgagct tcacattcat ggcaacacca ccggaaaaaa
     2461 cataattcct gatgccggtt tcacgatgaa tatttgtcag cagctcggtt gctgtttttt
     2521 ccagccacat ctgaacgccg gccgcaacat tatcaaaacg atagtattga aagcgttcct
     2581 tcatgaactg aaacaggtcc cgaggacgat tcttgtgaac aatccgcatg ccgtttactt
     2641 gcgcaatctc gctgagttcg tctgccacgc gctcagcgtg ctcttctttg gcgtaaggag
     2701 caagacccat cactttgtac tcgtgttcat agggacgcat tcccagatag agagtgaccg
     2761 ttctgtaaac gcgagccagc tcgcattctt ttgtatccgc gaggagttga aacttgtctc
     2821 cctcgatctt ccagactgtt tgattgcgat tgtccccgaa cgcatctatg gtaaaaccaa
     2881 tcgtaggatt tgattttctt ggattcgaat aatatccata gtaaatatgg caggagtggt
     2941 gatcgtaaaa ccgaacacga tcttcaggta ttccgaggaa tttcgaaata catctttttc
     3001 gaatgcgatt gaactcctca agctgctcca caccactgag tttcaatagg gactcaggat
     3061 actcatagac gtcatcctgg tgctggaacc tcttctgagt cttgaaaaga ttcagattgt
     3121 agtctttgtc aaactcctgt ccgctcagct tcggcttcca gtacttctcc atctcctcga
     3181 tgtagtcgcg aatcgtaaaa acagaatgct ttcgcatcat caaatcgacg taatcatgat
     3241 gaaccccggc aatcgcgagt tcatcaatgt cctcaggacg aatattgtat gttttcagga
     3301 tgtgttcaat ggtgtagcgc ggaaatgctt cctcgttctt tcgtccggta aaccgttctt
     3361 cctgcgcggc aaaaacgatt tcaccgtcct tataaagcgc ggcagacgcg ttgtgatcgt
     3421 cataaatacc aataataatc atttttcact ccagttttgt cagtatagca ttgcgtatga
     3481 attcgtgcgc gggcacatcc ggatatggca taaaataggc gcgcgcgggc cctgactgtt
     3541 tctcatagtc catatcagac atgttcagca aatcgtctaa cgccctggca aaagaagaat
     3601 aatccgcccg gttaatatgc cagggtccag tcgtcggagg atcgtattgg gcaagacccg
     3661 agaaattaga gaacaaaact ctttgccccc agccgaatgc ctcacgaccc gccgtggatg
     3721 cgctcgtgac tgcaaccaga ctctgatcca tgatccgata tgtgctccgc gaaaccggat
     3781 cgaagtctgc cagatgagtt gcttcgccca gaagacgttt gtaatattcc ttctctcctg
     3841 gattcggaga gcgtgtagca acacagacac gcgctttcga atttctagtc cagcgccgca
     3901 gaaagtaatg aagaaccaga atcgcgcgtt tcagttccgg tatttcgaaa tcccggtgaa
     3961 ctccgggata ccaatcagaa ataaagcaca catcaaactc gcgcgagctt cctgaccacg
     4021 gaacgcgttt gcgatagtac cccgcgatga gagaaccaac cggatgaaac tgtttcaccg
     4081 gaacttcata acggcgaaaa atatcgatat caaactgact gtagcaaaag aactccggca
     4141 gaggcagaat aggttcgccg gtattccagc gttcttccac tggaagacgc gtgccattct
     4201 gaactgcaca aaacgtggcc gccccggaaa actctgcaat ccaacgaaaa tggtgattgt
     4261 tgtcaatatg cgtgacgact acacgggctc ccgatgcgcg aatgcaagca gtcaggtaca
     4321 cgtgaaatga acgtgatgca atgtcacgat agaacctggt tcgaagaaag aagatcctta
     4381 cttccagaag catgtaccag agcacgcgcg cgcccaataa atactcttca ccgcgcaccg
     4441 gcagcacaca tgcttttacg tcaggcagac aatatcccag aagatactga cttccttcgc
     4501 gatcaaaaat ggcaagcttg ctctggattg gactgcgaaa cctcgcgttg cgcaaaacgc
     4561 gcctcaggcg accggccaga tagaaagcaa gtgccacata tctcaatagt cgcctcatgc
     4621 cggatttggg cgctgcgaaa gctggaactc ggccatagcc cagtcaagcg gagt
//
LOCUS       DAORFF010000293         4622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10720_length_4622_cov_3.126341, whole genome shotgun sequence.
ACCESSION   DAORFF010000293 DAORFF010000000
VERSION     DAORFF010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10720_length_4622_cov_3.126341"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PKK76_11990"
     CDS             <1..978
                     /locus_tag="PKK76_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69242.1"
                     /translation="LSFILALGTCVDLAIRYPVAAPSSSAHLLDSQTMAGRILLNAGV
                     LLDEDNDGNSVWPGGDPDDRDPCIRGDGLNVCKGSPPLDLPPPLHNRILLVTAASNEA
                     EGRPLFIPGDSMTVVLKAMLRGVDGYSAEMRKPSDPLLALISEYGFRTICVSVHWKDL
                     LPESGNGFDHGCQVVLEANDLKSAIDAAEKYSRKKFFVWIHLDRLDVGQTAVLKERIS
                     QYRYAIIRMNLAKQTAYAETKDCPIPLHLRSILPALAADTEAPGDPVDVRSNSSVRAP
                     ALYRLLGLHHARFFYSIKYSRKQDGTPYRRAFEGYTGAVWISEAGETID"
     gene            complement(1002..2279)
                     /locus_tag="PKK76_11995"
     CDS             complement(1002..2279)
                     /locus_tag="PKK76_11995"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNL69243.1"
                     /translation="MDTAIFSLGPATIDSTVTVPQFINDDERVLIDLQLSSVKAAFAS
                     GGPTSFENAGPRRKIFFEPSRTKAAIVTCGGLCPGLNNVIQSVVRMLYFSYGVRQILG
                     VRYGYEGLIPDFGHSFLELTPQLVHDIHKTGGTILGTSRGPQDIGRMVDTLVANKIDI
                     LFTLGGDGTLRGADAIHEEIKRRGLKISVAGIPKTIDNDIQFAHKTFGFATAVSIANE
                     SVHAAHAEAKSARNGIGLVKVMGRDSGFIASYVALASNEVDYVFVPESHFELRGEKGF
                     LPHLEKRLAESKHAVVLVAEGAGQKYCEITGEKDASGNVKYGDVGVVMSTEIKKYFKE
                     KNIPVVLKYIDPSYIIRSVPASADDAVFCIMLAQNAVHGAMAGKSGFMVGYWNSYYTF
                     VPLKLATSGRKKVDPKGYLWTVVCEATGQPDFF"
     gene            complement(2281..4335)
                     /gene="glgX"
                     /locus_tag="PKK76_12000"
     CDS             complement(2281..4335)
                     /gene="glgX"
                     /locus_tag="PKK76_12000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNL69244.1"
                     /translation="MKIEATLPGRSFPPGSTVLPEGVNFCVFSKNCQFIELLFFDDVN
                     AKEPSRIIPLHPENNRTFYYWHILVKGIKPGQLYGYRAQGPFEPETGLRFDGEKILID
                     PYGKAVAVGDSYDRTAAQKPGSNAHCCMKSVVVDTASYDWEGDMPIHRDLRHSFIYEL
                     HVGGFTRSMDSGIAEEKRGTFAGIIEKIPYLKSLGVTEVELLPVQHFDAQDAPLGKPN
                     YWGYSPVSFFAPHSGYATHSDPQAPVREFRDMVKALHRAGIEVILDVVFNHTAEGDHT
                     GPTLSFRGLENRAYYIPDPENRSRYANYSGCGNTFNANQSIARRMILDSLRYWVSEMH
                     VDGFRFDLASVLSRDEWGAPLRSPPILWEIESDPVLAGTRIIAEAWDASGLYQVGSFI
                     GHKWAEWNGIFRDDVRRFVRGDRGLVPKLMQRITGSPDVFPQKEWEPERSINFLTCHD
                     GFTLNDLVSYNQKHNESNGEQNRDGMDENYSWNCGEEGISSNPLVNALRKKQIKNFLT
                     LLFISQGTPMIQMGDEAARTQNGNNNAYCQDNETSWFRWKDLETNAEILRFQRELAEF
                     RKANTVFHMPRFWTSGSPEKPVLRWSGVRLWEPDTGHVSHSIAFQIQEPEKSEKIFVI
                     LNAYHGPLDFELPPSETGWKRAIDTSLPAPDDICPPSSRPVIPGKQYTANERTFIVLT
                     TG"
BASE COUNT         1048 a         1148 c         1209 g         1217 t
ORIGIN      
        1 ttgtctttca ttcttgcact cggaacatgc gtcgatcttg cgattcgcta tcctgttgcc
       61 gcaccatctt catctgcaca tttgctggat tcgcaaacaa tggcagggcg catcctgctc
      121 aatgcaggag ttctcctgga tgaagacaat gacggcaatt cagtctggcc aggcggcgat
      181 ccggatgacc gtgatccatg cattcgcggc gacggtctga acgtatgcaa gggcagtcct
      241 cctctcgacc ttccgcctcc gttgcacaat cgcatcctgc ttgtgactgc tgcttcaaat
      301 gaagcggagg ggcggccgct gttcattccc ggtgattcca tgacggtagt tctcaaggcg
      361 atgcttcgcg gcgtggatgg ctactcggca gagatgcgaa agccttccga tccgcttctc
      421 gcattgattt cagaatatgg ttttcgcacg atctgcgtga gtgttcactg gaaagatctt
      481 ttgcccgaat ccggaaatgg atttgatcac ggatgccagg tagttctgga ggcaaatgat
      541 ctcaaatctg caatcgacgc cgcagagaag tactctcgca agaagttctt cgtctggatt
      601 catctggacc gtctggatgt agggcagact gccgttctaa aagagcgaat ctcccaatac
      661 cgttatgcga tcattcgcat gaatttagca aaacaaacag catacgcgga gacgaaggat
      721 tgccccattc ctctccatct gcgttccatt ttgccggctc tggccgcgga cacggaggcg
      781 ccgggggatc cagtggatgt tcgctcgaat tcttcggtca gggctcctgc cctctacaga
      841 ttgctggggc tgcaccacgc caggttcttc tattccatca agtattcgcg taagcaagat
      901 ggcacaccgt acagacgagc ttttgaaggg tatacgggag cggtttggat ttcagaggca
      961 ggcgaaacga tcgactgagt ctgatcgcct ttgcccttcc tctagaaaaa atccggttgc
     1021 ccggttgctt cgcataccac cgtccacagg tagcctttgg gatccacttt cttgcggcca
     1081 gacgttgcga gcttcaatgg aacaaaggtg tagtaggaat tccagtatcc caccatgaag
     1141 cctgatttgc ccgccatcgc tccgtgaact gcgttttgag cgagcatgat gcagaagacc
     1201 gcgtcgtctg cactggcagg aacactgcgg atgatgtaac ttggatcaat gtatttgaga
     1261 actacgggga tgtttttttc tttgaaatat ttcttgatct ctgtgctcat cacaacgccc
     1321 acatcgccgt atttcacatt tccagatgca tccttttctc ctgtgatttc gcaatacttc
     1381 tggcccgcac cttctgcaac aaggacgact gcgtgtttgg attctgcgag tctcttttcc
     1441 aggtgaggga ggaatccctt ttcgcctcta agttcgaaat gtgattccgg aacgaaaaca
     1501 taatccactt cattggaagc gagcgcaacg taagatgcga tgaagccaga atcgcgaccc
     1561 atcactttga caagaccgat cccattgcgc gctgattttg cttccgcatg tgccgcgtga
     1621 acggactcgt tagcaatgga aacggcagtt gcgaagccaa atgttttgtg agcgaattga
     1681 atgtcgttgt cgatggtctt tggaattccg gcgactgaga tctttaagcc gcgtcttttg
     1741 atttcttcgt gaatcgcgtc tgcgcctcgt agagtgccgt cgccgccgag cgtaaacaga
     1801 atatcaattt tgtttgcaac gagtgtgtcc accattctgc cgatgtcctg tggacctctc
     1861 gatgttccga ggatagtgcc gccggttttg tgtatgtcat gcacgagttg tggagtcaat
     1921 tccagaaatg aatgaccaaa atctggaatc agaccttcgt agccatagcg aacgcccagg
     1981 atctgccgga ctccgtaaga gaagtagagc attctcacca cgctttgaat gacgttgttc
     2041 agtccagggc ataaacctcc acacgtaacg atagccgctt ttgtgcgcga aggttcgaag
     2101 aatatttttc gacggggacc cgcattttca aagctcgttg gccctccgct agcgaaggct
     2161 gcctttacag agctgagctg cagatcgatc agaactcttt catcatcgtt gatgaattgg
     2221 gggactgtga cggtagagtc aatggttgcg gggccgaggc taaagattgc tgtgtccata
     2281 ttatcctgtg gtcaatacaa tgaacgtgcg ctcgtttgct gtatattgtt ttccgggtat
     2341 aaccgggcgc gaagaaggag gacaaatgtc atctggagca ggaagagatg tatctatcgc
     2401 gcgtttccag ccagtttcgg aaggcggcag ttcaaaatcg agaggaccgt ggtatgcgtt
     2461 tagaatcacg aaaatctttt cagatttttc gggctcctgg atctggaagg cgatcgagtg
     2521 cgaaacatgc cctgtatccg gttcccagag acggactccg gaccatcgca gaactggctt
     2581 ttccggggaa ccggaagtcc agaagcgggg catatggaat acggtgtttg ccttacgaaa
     2641 ttccgccagt tcgcgctgga agcgaaggat ctctgcgttg gtttccaaat ctttccagcg
     2701 aaaccaggac gtttcattgt cctgacagta tgcgttgtta tttccgttct gcgttcgcgc
     2761 agcttcgtcg cccatctgga tcatgggagt tccctgcgaa atgaacaata gagtgagaaa
     2821 attcttgatc tgttttttgc ggagtgcatt taccagtggg tttgaggata tgccttcttc
     2881 gccgcaattc cagctgtagt tctcgtccat cccgtctctg ttttgttccc cgttgctttc
     2941 attgtgtttc tgattgtagg aaacgagatc gttgagagtg aatccgtcat ggcacgtgag
     3001 gaagttgatg cttcgttcgg gttcccattc tttttgcgga aatacatcag gacttcccgt
     3061 gattcgttgc atgagttttg gaacaaggcc tctgtccccg cgaacaaatc gacgaacatc
     3121 atcgcgaaag attccattcc attccgccca cttgtgaccg atgaacgatc caacctggta
     3181 gagcccggat gcgtcccatg cctctgcaat gatgcgagtg cccgctagaa caggatccga
     3241 ttcgatttcc cacaggatcg gtgggctgcg aagtggcgct ccccattcgt cgcgcgaaag
     3301 cacggacgcc aggtcaaaac gaaatccgtc tacatgcatt tcagaaaccc agtagcgcag
     3361 gctgtccagg atcattctgc gtgcaatgga ctggttcgca ttgaatgtgt ttccgcaacc
     3421 actgtagttc gcatatctgg atctgttttc aggatctgga atgtagtagg ctcgattttc
     3481 aaggccgcga aaggaaagag taggccctgt atgatctcct tctgctgtgt gattgaaaac
     3541 cacatcaaga attacttcaa ttccagcgcg atggagtgcc ttaaccatat ctctgaattc
     3601 gcgtacaggt gcctgcggat cagaatgagt cgcataaccg gagtgaggcg caaagaagga
     3661 cacagggctg tagccccagt agttgggttt ccccagagga gcatcctgcg catcaaaatg
     3721 ctgcactgga aggagctcca cttctgtaac tccgagtgat ttcaaatagg gaatcttttc
     3781 aatgattcct gcgaatgttc ctctcttctc ctctgcgata ccgctgtcca tgcttcgagt
     3841 gaaaccacca acatggagtt cataaatgaa cgagtgacga agatccctgt ggatcggcat
     3901 gtctccttcc cagtcatagc tggctgtgtc cacgaccacg ctcttcatgc agcaatgagc
     3961 gttgctccct ggcttttggg cagcagtgcg atcataggaa tcaccgactg ccactgcctt
     4021 tccatacgga tcgatcagta tcttttcacc gtcgaagcgc agtcctgttt ctggttcaaa
     4081 cggcccctgg gcgcgatacc catagagttg accgggcttg attcctttca caagaatatg
     4141 ccagtagtag aaggttcgat tgttttcagg atggagtgga atgattcgtg agggttcctt
     4201 tgcgtttaca tcatcgaaga acaaaagctc gatgaattgg cagttcttcg aaaagacgca
     4261 gaaatttaca ccttcgggca gcaccgtgga accgggtggg aaggatctgc cgggcagtgt
     4321 tgcttcaatc ttcatgctcg cctcacatga gtgcaagagc cgacgggctc aagtgaattt
     4381 cgtgaatttt cgctatccgt atccgcaact cgttttgagc atgggatgaa atggcttccg
     4441 gacgacaaca ggtgcaggtt cgatacgtgg acgcggaggc gcgaaatcgt ttacgggctg
     4501 tttttgaaaa cctggaagca gacctgtaga ttgatggcat tggagtcgtt cttacgaccg
     4561 ctgtgcttga gcttgtggag aatgcagtca aagccaactt gaagcgcgcg ttttttagca
     4621 ag
//
LOCUS       DAORFF010000294         4621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10726_length_4621_cov_3.692510, whole genome shotgun sequence.
ACCESSION   DAORFF010000294 DAORFF010000000
VERSION     DAORFF010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10726_length_4621_cov_3.692510"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="PKK76_12005"
     CDS             complement(<1..1825)
                     /locus_tag="PKK76_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69245.1"
                     /translation="MGSQDHHEGHRFLEAIAESHAASVGVNLSEKHNHAVFRKVQSLS
                     TPQAARSGAYFTVGPIAFDGIGQIPRKELAAVLKNLTRDRLVVQVLRFEFENGAIVFK
                     PCFLARPQDRPESMRQIYEEIISQSATAFEKWMIQSPVTAVQLAREMQVDLSAGKEFS
                     FNSLTSFLSLDTFVNVRLFDVLPDSEMIEAVLGDIRTSLLERNLCVYVAGYGILKLAG
                     ANAADLFDAASDFMIDRVAPRYRNRGNCRRELAQIAVEEGIYHIDEFVPETPDFLVCR
                     AEELRRVGPGDPPESPNGRYAGAMAVEIVVALAPTVKEKYLADWRAENERSIENIKKK
                     LTGVNAAWLDLMSFTDQKDLMKMNPANWNRLVGDESLLYGAWELPRTTMHILVHRKSS
                     VFRRIVRGMVDSVAPREWQVLALKDLIETYEDYFKDLFLDREFVQMYGQLLRRAYLPY
                     MNWFFRLLVALNIKLFQDVAFQGAKKRIARHQAVLEARNHERYLELMKQREEERFKKT
                     NQIKRMALSNRMIDRLDYYYFQEGSLPTVGRLRAELGNISEEDFEALLKEERFQVITM
                     NRGDSRDDRLLLYPLDGGWKAKSARLAEALDAQEKEYDRPDA"
     gene            complement(1864..2082)
                     /locus_tag="PKK76_12010"
     CDS             complement(1864..2082)
                     /locus_tag="PKK76_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNL69246.1"
                     /translation="MRIKVRVKPGSKKPGIEKTEDGWIIRVRERAVENKANEAVIEAA
                     AQELGIPRSRLRIVLGATSRTKVIEADC"
     gene            complement(2100..2987)
                     /locus_tag="PKK76_12015"
     CDS             complement(2100..2987)
                     /locus_tag="PKK76_12015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNL69247.1"
                     /translation="MARLQKKSARSPSRSEKRAGSSVVLLSASIITFNEEHRIFDCIA
                     SVHDFCDEVLVLDSFSTDKTETIARKFPKVRFYKHAFDGHVQQKNRAFEMCRGKWILS
                     LDADERCDPELASSIQEFLRTSADHGPDGMRINRLTIHMGRPIHHGGWYNARYRLIKK
                     GKAAWGGENPHDQIFLHDEPRWKTNLGPVLSGNLVHYSFKDLSDQIGTINKFSSIIAF
                     TRKGKGKRFSLIRLLWKPFQKFLEIYLLKRGFLDGMPGFVIAYSSAFSYFLREAKLYE
                     LHHGLDRPSNLRPDYKVQR"
     gene            complement(2987..3565)
                     /locus_tag="PKK76_12020"
     CDS             complement(2987..3565)
                     /locus_tag="PKK76_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNL69248.1"
                     /translation="MPLKKTLFLAWVLLAVFACRPAARSNFGVDQWSGRLLDGSVIHF
                     SQIDAPKLVLNFYSPTCQPCIQEIPALEHFAAEAAKRKIPVYVALESSPSSHGLNLPA
                     DATPEDRFQAIRNRMMEDIQKYSMRLPVVVMDDNFRINPEYGTVTGTPETLIFSTKPL
                     LLRYNFIGPIGTAQDAATIERQPRYQFALERL"
     gene            3608..4102
                     /locus_tag="PKK76_12025"
     CDS             3608..4102
                     /locus_tag="PKK76_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNL69249.1"
                     /translation="MSRDAKEFEISKLLQLAEKEKYATTVAAFEVIDKLEQIDIPKKY
                     TNRKPAVKAMIALSDGIVQYDYIADEARQALLEELHGPGATSASPTAEIAAARGRVPV
                     VEESEEDLGDEDIGEIEPTAKLDEDSEDGDESEAADEEEEEDEDEEDSDDDDDEEDSE
                     PEDD"
     gene            4095..>4621
                     /locus_tag="PKK76_12030"
     CDS             4095..>4621
                     /locus_tag="PKK76_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69250.1"
                     /translation="MIDPSPGFSPNPDGKPNYTVDGKPLHSRNNPEEEARRFIAGILS
                     QNPTGNIIIAGVGAGYVFEILRHLPAERVFFLEPIGAIRERLLGVAPPGSRILASIED
                     LPSGPAQIFPLPGHRARFPIAHAAINDYAKSLSREPTETRIDRATTARFFRRWSRNFF
                     RSLFYDRLEFISQPV"
BASE COUNT         1187 a         1225 c         1097 g         1112 t
ORIGIN      
        1 ccgcatccgg gcgatcgtat tctttttcct gcgcatcaag agcctcagcc agacgcgcgg
       61 actttgcctt ccagcctcca tccagaggat agagcaaaag ccgatcgtct ctggaatccc
      121 cacggttcat tgtaattacc tgaaatcttt cttctttgag aagcgcctcg aaatcctctt
      181 ctgaaatgtt tcccagttct gcgcgaaggc gtcctacagt gggaagacta ccctcttgaa
      241 aatagtagta atccagacga tcaatcatac gattcgaaag ggccattctc ttgatttgat
      301 tggtcttctt aaatcgttct tcttctctct gcttcatcaa ttccagataa cgttcatggt
      361 ttcgcgcttc aagcacggcc tgatgacgag cgattctctt cttcgcgccc tgaaacgcta
      421 cgtcttgaaa gagcttgata ttcaatgcaa ccagcaatcg aaagaaccag ttcatgtacg
      481 gaagataggc tctccgcaaa agttggccgt acatctgaac gaactcgcga tccagaaata
      541 aatctttgaa atagtcctcg tatgtttcga tgagatcctt caaagccaga acctgccatt
      601 ccctgggcgc aacggaatct accatgccgc ggacaatcct tctaaaaaca gatgattttc
      661 tgtgcaccag aatgtgcatc gtggttctgg gcagttccca ggcgccgtac agaagacttt
      721 catctcccac aagacgattc caattcgccg ggttcatctt catcaaatct ttctgatccg
      781 tgaagctcat caaatccagc cacgccgcat tcacgcctgt gagctttttt ttgatgttct
      841 caatgcttct ttcgttctcc gcgcgccagt ctgccagata cttctctttt acagtggggg
      901 ccagagccac gacaatttcc actgccatcg cgcccgcata acggccgttt ggcgattctg
      961 gtggatctcc agggcccact ctccgcaatt cttctgcgcg gcaaaccaga aaatcaggtg
     1021 tctccggaac aaactcgtca atatggtaaa tcccttcctc caccgcaatc tgcgcgagtt
     1081 cgcggcgaca gtttccccgg ttgcgatagc gcggcgccac ccgatcgatc atgaaatcag
     1141 acgcagcgtc aaaaagatcc gcagcattgg ctccagcgag cttcagtatt ccgtaaccgg
     1201 ccacatacac gcatagattt ctttcgagca gagaagttcg aatatcgccc agaactgcct
     1261 cgatcatttc cgaatcgggt agtacgtcga agaggcgaac atttacgaac gtatcgagcg
     1321 acagaaagct ggttaaggaa ttgaaagaaa actcctttcc cgcgctcaga tcaacctgca
     1381 tctcccgggc cagctgtacc gcagtcacag gcgattggat catccatttt tcaaatgcag
     1441 tggctgactg agaaataatc tcctcataaa tctgccgcat ggactccggt ctgtcctgag
     1501 gtcgcgcgag gaaacatggc ttaaagacaa tcgcaccgtt ttcaaattca aatctaagaa
     1561 cctgaaccac aagacgatcg cgcgtcaggt tttttagaac cgctgcgagc tctttgcgtg
     1621 gaatctgacc aatgccatca aatgcaatgg gacccactgt gaaataggct ccgcttcgag
     1681 ccgcctgagg agtggaaaga gactgcacct tcctgaaaac cgcgtgattg tgtttttcag
     1741 aaagatttac tccaacgctg gcggcgtggc tctccgcaat tgcctcgaga aatcgatggc
     1801 cttcgtgatg gtcctgggag cccacgctca gaagttgaaa ggcgcgcaga atccttcaag
     1861 aaattaacag tctgcttcga tcactttcgt gcgcgaagtt gcaccaagca caattcggag
     1921 acgactgcga ggaatgccaa gctcttgtgc tgcggcttct atgactgcct cgtttgcctt
     1981 gttttccaca gcgcgctccc tgacccgaat gatccagccg tcttccgtct tttcgatccc
     2041 gggtttcttg ctgccaggct ttacgcggac tttgattcgc aaacagacct caaaagcgac
     2101 taacgttgta ctttatagtc tggacgaaga ttggatggtc gatcgagacc gtggtgaagt
     2161 tcatacagct ttgcttccct caggaagtag gaaaaggcgg aagaatacgc gatcacaaat
     2221 ccaggcattc cgtccaggaa gccacgcttg agcaggtaga tctcaaggaa tttctgaaat
     2281 ggcttccaca ggagtcgaat cagtgagaat ctctttcctt ttccctttcg agtgaaggca
     2341 atgatgctcg aaaacttgtt gatggtgcca atttgatccg aaagatcttt gaaagaatag
     2401 tgtaccaggt ttccggaaag aaccggacca agatttgtct tccacctggg ttcatcgtga
     2461 aggaagatct gatcgtgcgg attctctcct ccccaggcag ctttcccttt cttgatcaat
     2521 cggtaacgcg cattgtacca accaccatga tgaatgggtc tgcccatatg aatcgtaagg
     2581 cgattgattc gcattccgtc cgggccatga tccgcggatg tgcgaagaaa ttcctgaata
     2641 gaggacgcaa gttctggatc acacctttca tctgcatcga gagaaagaat ccattttcct
     2701 ctgcacattt caaatgcccg attcttctgc tgcacgtgac catcgaaagc atgtttgtag
     2761 aaacgtactt tggggaactt gcgagcaatc gtttctgttt tatccgttga gaaagagtcc
     2821 agcacaagca cttcgtcaca gaagtcatgc acggacgcaa tacagtcaaa gatgcgatgc
     2881 tcttcattga aggtgatgat ggacgcagac aaaagaacca cggatgaacc agctcgcttt
     2941 tctgatctcg atggagaccg cgccgatttc ttctgcaatc ttgccatcaa agtctctcca
     3001 gtgcaaactg gtagcgtggc tgtctttcaa tcgtcgccgc atcttgagct gtgccgatgg
     3061 gaccaatgaa attatatcgg agcaagagcg gtttcgtgga gaagatcaga gtctcaggag
     3121 ttccagtcac agttccatat tctggattga ttcgaaagtt atcgtccatg actaccactg
     3181 gaaggcgcat cgaatacttt tgaatgtctt ccatcatgcg attgcgaatt gcctggaatc
     3241 tatcctccgg ggttgcgtcc gccggtagat tgagaccgtg cgaagaagga ctggattcaa
     3301 gagctacata caccggaatc tttcttttag ccgcttccgc ggcaaagtgc tcgagcgctg
     3361 ggatttcctg aatgcacggc tggcaggtgg ggctataaaa attgagcacc agcttcgggg
     3421 cgtcgatctg actgaaatga atcacagagc cgtccagcag ccggccggac cactgatcta
     3481 cgccgaaatt ggacctcgca gcaggcctgc aggcgaagac tgctagcaaa acccaggcca
     3541 ggaacagtgt tttttttagt ggcataattg taccaaattg aaaaagtggc atttccttaa
     3601 aattaccatg tccagagacg ccaaagaatt tgaaatatca aaacttctcc agctcgcaga
     3661 gaaagagaag tatgccacaa ctgtagcggc tttcgaagtc atcgacaagc tggaacagat
     3721 tgacatcccc aagaaatata caaatcgcaa gcctgctgta aaggcaatga ttgcactgtc
     3781 agacggaatt gttcagtacg actacatcgc agatgaggcc cgtcaggctc tcctcgaaga
     3841 actccatggc ccgggcgcaa cttctgcgtc tcctactgca gaaattgcag cagcgcgtgg
     3901 aagggttcct gttgtagaag aatcggaaga agatctcggc gatgaggaca ttggcgaaat
     3961 cgaaccaacc gcgaagctcg atgaagacag cgaagacgga gacgaatccg aagcggctga
     4021 cgaagaagaa gaggaagacg aggacgaaga ggactctgac gacgatgacg acgaggagga
     4081 ttcagagccg gaggatgatt gatccctccc caggcttttc tccgaatcca gacggaaaac
     4141 caaactacac tgtagacggt aaaccgcttc actccagaaa caatccagaa gaagaagccc
     4201 ggcgattcat cgccggcatc ctttcgcaga atccaacagg caacatcatc atagcaggcg
     4261 tcggagccgg ctatgtattt gaaatccttc gccacctacc ggcagaacgc gtcttttttc
     4321 tcgaaccgat aggcgccatt cgagagcgcc tgctgggagt ggcaccgccc ggctcacgca
     4381 tccttgcctc catagaggat ctgccatctg gtccagcaca aatctttcct cttccaggac
     4441 atcgtgctcg atttccaatt gctcatgcag ccataaacga ttacgcaaaa agtctttcgc
     4501 gcgaaccaac ggagacccga atcgaccgtg ccacaaccgc tcgcttcttt cgcaggtgga
     4561 gccgcaattt ctttcgcagt cttttttacg atcgccttga gttcatttcg cagcccgtaa
     4621 a
//
LOCUS       DAORFF010000295         4612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10772_length_4612_cov_3.610928, whole genome shotgun sequence.
ACCESSION   DAORFF010000295 DAORFF010000000
VERSION     DAORFF010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10772_length_4612_cov_3.610928"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PKK76_12035"
     CDS             complement(<1..830)
                     /locus_tag="PKK76_12035"
                     /inference="COORDINATES: protein motif:HMM:NF028249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HNL69251.1"
                     /translation="MIRTCAFAIWTSLIFASAAHLSAETSITVSMDRTWNDGETVFEI
                     GSKQPDITGIAGGSRITYPRDFAYTRMALGFYSGKVGIDLDVGATGHAVRSGTGRDED
                     FFLYSTSRVDGAKVSLQDGRFSDRTRVFSGGRNWADSSGKTALVEYRARLITNYFPWS
                     DASPFHRDSGTFFALGFEHIYSKYRIYDSIQYNVVSVTPTNPYPFNLTPIGSGLSFTN
                     VSGELMLGAGVALYPWEAVGFSFSLSPLVGYANSRDYHSLRGITFIMENAGSGFLYQM
                     "
     gene            complement(830..2044)
                     /locus_tag="PKK76_12040"
     CDS             complement(830..2044)
                     /locus_tag="PKK76_12040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNL69252.1"
                     /translation="MDFELNQEDKEFAEHFRRFCEKEIGAVAGEVDESGSVPRSHYEA
                     LGAIGYNGLLHRREFGGQEASIVQATVAQMILAEACGSTFFSVGASCGLFGGPVASYA
                     SAAQKKSILPTLLAGKLIGCLAVTEPDTGSDVSAIRTRAKRSANGFVLNGQKTFITNA
                     PIGDYAVVLARLGEENADSKGSLSCFLVDLHLPGVTRGKAMRKMGLRASPTGELFFDN
                     VELAAESLLGAPGQGFRMVMEAFAMERLALAAYCVGTMKACFDDSRRYSRERKAFGRP
                     IAKHQSVAFMLADIKTKLEAAYLLLMETAWLVDRQRAEAVGGKSPSRLMHNGYVIDIN
                     ARAAETKLLASTYAREVTNLAVQVHGGAGYMEEYRVCRLYRDIKIAEIGGGTSEIQKQ
                     IVAHAEIKRVGV"
     gene            complement(2073..3218)
                     /locus_tag="PKK76_12045"
     CDS             complement(2073..3218)
                     /locus_tag="PKK76_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNL69253.1"
                     /translation="MDFSLSKELVDFRESVRQFAIQHVKPTAEQRDLDERWDPDIWRK
                     MGEFGLLGLCFPEEYGGSGADCLTTTVATEAFAEGATDGGLTLAWGAHTIIGTMPIVL
                     CGTKEQKERYVPKLASGEWVAGLGLTEPGSGSDAAGSMETRAEKKGDRYILNGAKMFI
                     TNGPIGDVFTVMAVTDKKKGPLGISVFLVEKNFPGFRVGKKLKKMGMRSSTTSELIFE
                     DCEVPAENMIEKPNSGFLRVGKATLEWERTVLVASAIGSMHHMLDESIRYAKARRQFG
                     EPIARFQAIQEKISRVRMKLDASRLLLYKSAVMKDQGKPAPIESSLAKVHATEAVIEA
                     AYEMGQIHGGYCFIHEYPVERAYRDCRLGTLGGGTSEVMRSIIAANL"
     gene            3311..4051
                     /locus_tag="PKK76_12050"
     CDS             3311..4051
                     /locus_tag="PKK76_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69254.1"
                     /translation="MKLLLLTGIQEELYPMIPEFRLVFSKETGLYQSANYPELFAATT
                     GPGVKKRKELREIFKTIIPDIIVNAGLIGTLTDDPLPAPGDLAKISSVVEFETHLVFP
                     VQPHGGTLLTVRKPVFEPREKWLLSRDYQARFCDMEASRILQLLREVDEVREDTSVVF
                     CKVVGDTPAQYDLYQHEELVRGWHRENWFGKMRIGLKFPGGPMRLRRLLALKEKSLDG
                     LTEHTRRTVKALLAGANPDKMGAVYAPA"
     gene            4053..>4612
                     /locus_tag="PKK76_12055"
     CDS             4053..>4612
                     /locus_tag="PKK76_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69255.1"
                     /translation="MVIVSVIIGLAIAGVAAYYLLFTDRRDVYERALNMAAEGHFVDA
                     RGLIRAKIEGHPDNSIGHYTMARVYALEGKTEDEKRHLLEVRRINAYGADVHPVSVLN
                     RIGDIEYSDGDYRASVDAYLATLQFSPQNEEALARVAFLTVGRSKFDIAERYFRTLVR
                     IAPNTLEYRIGRGVGLAMLKSKDAIR"
BASE COUNT         1125 a         1255 c         1145 g         1087 t
ORIGIN      
        1 tccatttgat acagaaaacc agatccagca ttctccataa tgaatgtgat gccgcgcaaa
       61 gaatgatagt cgcggctgtt cgcataacca accaaaggcg aaagagaaaa agaaaacccg
      121 actgcctccc atggatacaa cgccacgccc gcacccagca tcagttctcc gcttacgttt
      181 gtaaacgaga gaccactccc aatcggggtt aaattaaagg gatatggatt ggtgggagtg
      241 acggacacta cgttgtattg tatagaatca tagattctat acttggagta gatgtgctca
      301 aaaccgagtg caaaaaaagt tcctgaatcg cggtgaaagg gactggcgtc agaccacgga
      361 aagtagtttg tgatcagtcg tgctcgatac tcgacgagcg cggtcttgcc agatgagtcg
      421 gcccagtttc ttccgcctga aaaaacacga gtcctgtcac tgaatcgtcc atcctgcagt
      481 gagactttgg cgccatcaac acgagacgtt gaatacaaaa agaagtcttc atctctacct
      541 gtgccggatc gcactgcgtg ccctgtggct cctacatcga gatcaatgcc gactttccct
      601 gagtagaaac caagagccat ccgcgtgtat gcaaaatctc tgggatacgt gatgcgagaa
      661 ccgccagcga ttccagtgat gtccggttgc ttagacccaa tttcaaaaac agtttcgccg
      721 tcattccacg tgcggtccat ggaaacggtg atggaagttt ccgcagagag atgcgcggca
      781 gatgcgaaga tgagtgaggt ccatatcgca aatgcgcacg tgcgaatcat caaacaccga
      841 cgcgcttgat ttccgcgtga gcgacgatct gcttctgtat ctctgaagtg cctccaccaa
      901 tttccgcgat cttgatatcg cgatacaatc tgcacacccg atactcttcc atgtagcctg
      961 cacctccgtg aacctgcact gcgagattgg tcacctcacg cgcgtaagta gaagcaagaa
     1021 gctttgtttc cgcagcgcga gcattgatgt ctatcacgta accgttgtgc atgagtctgg
     1081 acggtgactt tccacccact gcttctgccc tctgtcggtc cacgagccat gctgtttcca
     1141 tgagcaaaag ataggctgct tccagctttg tcttgatgtc tgccagcatg aatgcaacgc
     1201 tctggtgctt cgcaatgggc cgtccgaaag cctttctttc ccgcgaatag cgacgtgaat
     1261 cgtcaaagca agctttcatg gtaccaacgc aataagccgc taaagcaagc cgttccatgg
     1321 cgaacgcttc catgaccatg cgaaagccct ggcctggtgc acccaggagg gattcggccg
     1381 caagttccac attgtcaaaa aacaactctc ctgtgggaga ggcacgcaac cccatttttc
     1441 gcatagcctt gcctcgcgta acaccgggaa ggtgaaggtc taccagaaag cagctcaagg
     1501 atcccttaga atccgcattc tcctcaccaa ggcgcgccag aaccactgcg taatccccga
     1561 tgggcgcgtt cgtaatgaat gttttttgcc cattcaaaac aaatccgttg gccgaccgtt
     1621 tggcacgggt gcgaattgca cttacgtccg aaccagtgtc tggctcagta accgcgaggc
     1681 atccaatgag ttttcccgca agcaaggttg gcagaatgga tttcttttga gctgcagatg
     1741 cataggacgc cactggccca ccgaagaggc cgcaggaggc acccacagag aagaaagtag
     1801 agccgcaagc ttccgcgaga atcatctgcg caacggtcgc ctgcacaatg gaagcttctt
     1861 gcccaccgaa ttcacggcga tggaggagac cattgtagcc aatggcacct aacgcctcgt
     1921 aatgcgaacg cggcacactt ccagattcat ccacttcacc ggctacagcg ccaatctctt
     1981 tttcgcagaa gcgtctgaaa tgctccgcaa attctttgtc ttcttgattc agttcaaagt
     2041 ccataattac accacaattc cgtttgaatg tctcacaggt tggccgcgat gatagatctc
     2101 atcacttcag aagttccgcc acccagagtg cccagtctac aatcgcggta tgctctctcc
     2161 accggatatt catgaataaa gcaatagccc ccgtggattt gtcccatctc gtaggcggct
     2221 tcaatcactg cttcggtcgc atgtactttt gcgagtgaac tttcgatggg cgcgggcttt
     2281 ccctggtctt tcatcactgc actcttatat agaagcaatc ttgacgcatc gagtttcatg
     2341 cgaaccctgg agatcttttc ttgaattgcc tgaaagcgcg cgatgggttc accaaattga
     2401 cggcgggctt tcgcatatcg gatcgattca tcgagcatat gatgcataga tccgatggca
     2461 ctggcgacga gaaccgtacg ctcccattcc agagtggcct tgcccactct gagaaatccg
     2521 gagttaggtt tctcgatcat gttttctgcg ggcacttcac aatcttcaaa aataagttcg
     2581 ctggttgtgc tcgatcgcat tcccatcttt ttgagtttct tcccaacgcg aaagcctgga
     2641 aaattcttct ctacgagaaa gacggagatc cccagtggtc ctttcttctt gtccgtaacc
     2701 gccatcacgg taaatacatc tccaatgggt ccgtttgtga tgaacatctt ggcgccgtta
     2761 agaatataac gatccccctt tttttccgca cgagtctcca tgcttcccgc ggcgtctgaa
     2821 ccggatcctg gttctgtcaa acccaggccc gccacccatt cgccgctggc gagcttaggc
     2881 acgtatcttt ccttttgttc tttggtcccg cacagaacga tcggcatagt gccaatgatc
     2941 gtatgcgcac cccaggcaag agtgagtcct ccgtccgtgg cgccttctgc gaatgcctct
     3001 gtggccactg tggttgtcag gcaatcggca cctgagccgc catattcctc tggaaagcac
     3061 agcccaagta ggccgaactc acccattttt ctccagatat cgggatccca gcgctcgtcc
     3121 aggtcgcgct gctccgctgt gggcttcacg tgctggatgg cgaattgccg caccgattcg
     3181 cgaaaatcga cgagctcttt ggacaaagaa aaatccataa caccgattgc tttttcctag
     3241 cgggtgacgc caggcttttt ctgtgcagag cagatggaaa atctccgtat tataagacat
     3301 cccggccgat atgaaattgt tactcctcac aggcatccag gaagagctct atccgatgat
     3361 tcctgagttt cgcctggtgt tttcgaaaga gaccggcctc taccaatcgg ccaactaccc
     3421 cgaactgttt gccgcgacca cgggacccgg tgtcaaaaag cgcaaagagc tacgcgaaat
     3481 cttcaaaacg atcataccgg acatcatcgt gaacgcgggg cttattggaa ctctcacgga
     3541 tgatcctctg ccggctccgg gggatctcgc gaagatctca tcggttgttg aatttgaaac
     3601 gcacctggtg tttccggtgc agccacacgg aggaacactc ctgaccgttc gtaaaccggt
     3661 ctttgaaccc agagagaaat ggttgctttc ccgcgactat caagctcgct tctgtgatat
     3721 ggaggcatcc agaatcctgc aacttcttcg agaagtggat gaagtgcgtg aggacacctc
     3781 tgttgtattt tgcaaggtcg tcggggatac accggcccaa tatgacctct atcaacatga
     3841 agaactcgtg cggggatggc acagagagaa ctggttcggg aagatgcgga ttggtttaaa
     3901 atttccaggc gggcccatgc ggctccgcag attgctggcc ttgaaagaaa aatccctgga
     3961 tggtctgacg gagcatacgc gcagaaccgt gaaagcactt ctcgccggag ccaatccaga
     4021 caaaatgggc gcagtgtacg cgccggctta gtatggtcat cgtatctgtc atcattggtc
     4081 ttgccatcgc aggtgttgca gcatactatt tgttgttcac cgatcgccgc gacgtgtacg
     4141 agcgcgctct caacatggca gccgaaggtc atttcgtaga cgcgcgtggt ttgattcgcg
     4201 cgaagattga aggacatccg gataattcca tcggccacta cacaatggcc cgggtttacg
     4261 cactcgaggg aaagacagaa gatgagaaaa gacatctcct agaagtgcgg cgaatcaacg
     4321 catacggagc ggatgtgcat ccggtcagtg ttctgaatcg aataggggat attgaatatt
     4381 cagacggaga ctatcgcgcc agtgtagacg cttatctggc cacactgcaa ttctcaccac
     4441 aaaacgaaga ggccctggca cgcgtagcat tcctgactgt gggcagatcc aagtttgata
     4501 ttgcggaaag atactttcgc actcttgttc gcatagcacc taacactctg gaatatcgca
     4561 taggacgcgg agtgggactg gcgatgctca aaagcaagga tgcgatccgc ga
//
LOCUS       DAORFF010000296         4597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10813_length_4597_cov_2.617129, whole genome shotgun sequence.
ACCESSION   DAORFF010000296 DAORFF010000000
VERSION     DAORFF010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10813_length_4597_cov_2.617129"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PKK76_12060"
     CDS             complement(<1..473)
                     /locus_tag="PKK76_12060"
                     /inference="COORDINATES: protein motif:HMM:NF014700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69256.1"
                     /translation="MAFFEYSAQISAPARAKRTRKLILLGASGSIGSSTLSILPESGI
                     ELVAVSVHGKADRLAEIISKFPTIRYAAITDPEMAQGLARLSASFPSVQFFSGSSGLV
                     ELVRLGAQAGADTVLTAVMGSAGIAPTLEAIQIGLKIALANKETLVTAGPVIENAL"
     gene            complement(480..1394)
                     /locus_tag="PKK76_12065"
     CDS             complement(480..1394)
                     /locus_tag="PKK76_12065"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNL69257.1"
                     /translation="MGETSKRIISGVTLGAAIILALNLPDFLYAVLILGLILAASLLG
                     VVEFYGLSDRGLDGRPVKGVGIFFAVLLIFTYYLQYLGIRATNGHPAPSFLTPDIAYV
                     ANHSVPFVLMLLVIVAMSAHLIFRPLDGTVYGVTTTIFGVIYPCMTLCHAFLILAEER
                     GLFFFVVVALATIGTDTGAYFAGRYFGKHNAGLKVSPRKTIEGYVGGFVFSILLMLGF
                     LYCWKRFAYEIHMGYVEMALFTAVLSVISVFGDLVESAIKRDSKKKDSAALIPGHGGM
                     LDRGDSLYFTIPIGYFYLYIRGIAGLTL"
     gene            complement(1403..2140)
                     /gene="uppS"
                     /locus_tag="PKK76_12070"
     CDS             complement(1403..2140)
                     /gene="uppS"
                     /locus_tag="PKK76_12070"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNL69258.1"
                     /translation="MSQENTNPRHVAIILDGNGRWAKKRGLSRSEGHRAGGEALDRLL
                     DDALEIGIPFISLYAFSTENWKRPRAEIQAIWKLMREFFTDRLQRCIEKGIRVHASGD
                     LSRLPAANRKLILHVIEKTAKNKALTANFCVNYGSMDEISHAATEIVRERLSLAKKGK
                     KADAAVSAKEIEKHLYTHPIPPVDLLVRPGGESRLSNFLLWQCAYAELYFTDVLWPDF
                     GRRDLQAALKWFRSRPRKFGGLLAKED"
     gene            complement(2142..2708)
                     /gene="frr"
                     /locus_tag="PKK76_12075"
     CDS             complement(2142..2708)
                     /gene="frr"
                     /locus_tag="PKK76_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNL69259.1"
                     /translation="MPDKPVDSAAVLAELKAKMQKSLDSLVKDLAGIRSTRANAAMLD
                     GVHVEYYGSSVPLNQVASLSVPEARMLVITPFDKSALGEIEKAILKSDVGITPSNDGS
                     LIRLILPELSMERRQELVKQVKVRLEECRVSIRNLRRDGNEEIKKIAGKSQDETKSLQ
                     DDVQKLTDSFIHKAEDAAAQKEKGILTV"
     gene            complement(2701..3441)
                     /gene="pyrH"
                     /locus_tag="PKK76_12080"
     CDS             complement(2701..3441)
                     /gene="pyrH"
                     /locus_tag="PKK76_12080"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNL69260.1"
                     /translation="MNPNFPFRRVLLKLSGEAFTTEGKLGIEFSRVRHIAEQIKAVHR
                     MGAEIAVVVGAGNIFRGQAAAESGMDRAQADYMGMLATVLNALSLQDACEKVGVITRL
                     QSAIEMKSICEGYIRRKAMRHLEKNRIVIFAGGTGNPYFTTDTTAALRAVEVGCQVIL
                     KATKVDGVYDSDPKKNPDAKRFSRVSYMESIQRRLKIMDSTALALCMDNNTPILVFDI
                     FEPDSLKNLVDGKKIGTLISSEEGLEYA"
     gene            complement(3456..4049)
                     /gene="tsf"
                     /locus_tag="PKK76_12085"
     CDS             complement(3456..4049)
                     /gene="tsf"
                     /locus_tag="PKK76_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNL69261.1"
                     /translation="MAEVKSDDIKKLREMTGAGMMDCKSALAENAGDIEKASEALRKK
                     GLAKAAKRMDRDTGSGRVFSYIHGEGKIGVLLQLNCETDFVAKNEEFEQLGKDLCMQV
                     AAASPLAIKAEDLDPKIIEKETEVYREQLIQEKKKPEQIEKILPGKIKKFQQDVCLLD
                     QFFIKDNKQTVAELIKSFIGKFGENITISRFTRYEAG"
     gene            complement(4064..>4597)
                     /gene="rpsB"
                     /locus_tag="PKK76_12090"
     CDS             complement(4064..>4597)
                     /gene="rpsB"
                     /locus_tag="PKK76_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNL69262.1"
                     /translation="MEAAGSFDQEARTKKEALLLQRELDKLRKHLSGIKELQSPPEIL
                     FVIDPSKEDIAVKEARAMGIKIFAVVDSNCNPELIDYPIPGNDDAIRAISLYLQTMAD
                     AVLEGTQGVEQGSRFTDEDATMDPDAIALDSLQYRGEYDESGEFIPDDANSPKTNPDE
                     EAAVKEYVAPPAAPKES"
BASE COUNT         1083 a         1181 c         1116 g         1217 t
ORIGIN      
        1 agtgcgtttt caatgaccgg tcctgcagtg acgagtgttt ctttgtttgc aagcgcgatc
       61 ttgagcccga tctgaatggc ttcgagtgtg ggcgcaattc cagcagaacc catcactgca
      121 gtcaaaacag tatctgctcc ggcctgtgcg cccaatcgta cgagttctac gaggccgctg
      181 cttcccgaaa agaactgaac ggaaggaaac gacgcagaaa gcctggccaa tccctgggcc
      241 atttctggat ctgtgatggc cgcataacgg atggttggaa attttgaaat gatctctgca
      301 agtctgtctg ctttgccgtg aacgctcacg gcaacgagtt ctattccgct ttctggtaaa
      361 atggaaagag tgctacttcc gatcgacccg gatgcgccga gcaagatgag ctttcgcgta
      421 cgtttcgcac gggccggtgc cgagatctgc gccgaatatt caaagaacgc catgctggat
      481 caaagagtca ggccagcgat gccacgaatg tacaaataga agtaaccaat gggaattgtg
      541 aaatagagag agtcccctcg gtcgagcatt ccgccgtgac ctggaatcaa ggcagctgaa
      601 tctttctttt ttgagtcacg cttgatggca ctttccacca gatccccaaa tacagaaatc
      661 acagacagca ccgcagtgaa gagcgccatt tctacgtaac ccatgtggat ttcgtaagca
      721 aatctcttcc aacagtaaag aaacccgagc atcaaaagaa tggagaaaac aaaaccgcca
      781 acgtaacctt caatcgtttt gcgcggactc accttcagac ctgcattgtg ttttccaaag
      841 taacggcccg caaaatacgc ccctgtgtcc gttccgatgg ttgccagagc cactactacg
      901 aaaaagaaca aacccctttc ctctgcgagg atgagaaacg catggcatag ggtcatgcac
      961 ggatagatca cgccaaagat cgttgtggtt acgccgtaga ctgttccgtc cagaggacga
     1021 aagatcaaat gcgcagacat ggccacaatt acgagcaaca tcaatacaaa cggaacagaa
     1081 tgattggcca cgtatgcaat gtctggagtt aaaaacgaag gagcgggatg tccgtttgtg
     1141 gcgcgaattc ccaggtactg cagatagtat gtaaatatga gtaatacggc aaagaaaatc
     1201 cccacgcctt tcaccggtct tccgtccaga ccgcgatcac tgagcccgta gaactccact
     1261 actccaagca aagacgccgc gagaatcaaa ccgaggatga gaacagcgta gagaaagtcc
     1321 ggaaggttca acgcaagtat gatagcggct ccgagcgtga ctccggatat gatgcgtttg
     1381 gacgtttcac ccatgaactt gtttagtctt ccttcgccag aagtccaccg aatttgcgag
     1441 gcctggatct gaaccacttc agtgccgctt gcaagtcccg gcggccaaaa tcaggccata
     1501 gaacgtccgt gaaatataac tctgcgtacg cacattgcca tagtaagaaa ttgctgaggc
     1561 gactttctcc tccgggtcga accagcaagt caacaggcgg gatcggatga gtgtatagat
     1621 gtttttcgat ttctttcgcg ctcacggctg cgtccgcctt cttccctttc ttcgcaagag
     1681 acaacctttc gcgcacgatc tcagtggcgg cgtgactgat ctcatccatg gagccatagt
     1741 tcacgcaaaa gttggccgtc agtgctttgt tcttcgccgt cttttcgatc acatgcagaa
     1801 tcaattttct atttgcggcc ggtaacctgg acaggtctcc ggaagcatgc acgcgaattc
     1861 ctttttcaat gcacctttgc agacgatcgg tgaagaattc gcgcatcaac ttccagattg
     1921 cctggatttc cgctcgaggt cgcttccagt tttcagtact gaatgcatag agtgatatga
     1981 atggaatgcc aatttcaaga gcatcatcga gcaaacggtc tagagcttct cctccggcgc
     2041 ggtggccttc ggagcgcgac agaccgcgct tctttgccca gcggccgttt ccatcgagta
     2101 tgatggctac atgccgcgga ttggtgtttt cctgggacac gttagaccgt cagaatgccc
     2161 ttctcttttt gagccgcggc gtcttctgct ttgtgaatga aactatctgt tagcttttgc
     2221 acgtcatctt gaagggattt cgtttcatcc tgggacttgc ctgcaatctt cttgatctct
     2281 tcgtttccgt cacgacgaag attgcgaatg gaaacgcgac attcttccag acgaaccttg
     2341 acctgcttga ccagttcctg gcgtctctcc atagaaagtt caggaagaat gaggcgaatc
     2401 agagagccgt cgttggaagg agtgattccc acatctgatt tcaaaattgc tttttcaatt
     2461 tcacccaggg cacttttgtc aaatggagtg attacgagca tccgcgcttc tggaacagaa
     2521 aggctggcta cctggttgag cggaactgag gagccgtagt attccacgtg caccccgtcg
     2581 agcatggccg cgtttgcgcg cgtagaacga atcccggcta gatcttttac aagagagtcc
     2641 aggctctttt gcattttggc tttcagttct gcaagaactg cggcggaatc aactggtttg
     2701 tcaggcatac tcgaggccct cctcggaaga tataagtgtt ccgatcttct tgccgtctac
     2761 gagattcttc aagctgtccg gttcaaaaat atcgaaaacc agaatggggg tgttgttgtc
     2821 catacagaga gccagagcgg tggaatccat gatcttgagt ctgcgctgga tggattccat
     2881 gtaggaaacg cgcgaaaaac gttttgcgtc aggattcttc tttggatcgg aatcatatac
     2941 gccatctact ttcgttgctt ttagaatcac ctgacagcct acttcgaccg cccgcagtgc
     3001 ggctgtcgtg tcggttgtaa aatagggatt ccctgttccg cccgcgaaaa tcacaatccg
     3061 attcttttcg agatgcctca ttgcctttct gcgaatgtat ccttcgcaga tggatttcat
     3121 ttcaatggct gattgaaggc gagtgatgac gcccaccttt tcgcatgcgt cctgcaaaga
     3181 tagagcgttg agcaccgtgg caagcattcc catgtagtct gcctgcgcac gatccattcc
     3241 actttctgca gccgcctgcc cgcgaaagat atttccggcg ccaacgacca ctgcgatttc
     3301 tgcacccatg cggtgcactg ctttgatttg ttctgcgatg tgacgaacgc gggaaaactc
     3361 aataccgagt tttccctccg ttgtaaaggc ttcacccgaa agcttcagta gaacccgacg
     3421 gaatggaaaa ttcggattca cgtgagcctc ctttatcagc ccgcttcgta acgggtaaac
     3481 cgggagattg taatgttctc tccgaatttc ccaatgaatg acttaatcaa ttctgcaaca
     3541 gtctgcttgt tgtctttgat gaagaattga tcgagaaggc agacatcctg ctgaaacttc
     3601 ttgatcttgc ctggcagaat cttttcgatt tgctctggct tcttcttttc ctgaatcagc
     3661 tgttcgcgat atacttctgt ttctttctcg atgattttag gatcgaggtc ttctgctttg
     3721 atcgcaagag ggcttgccgc tgcgacctgc atgcaaagat ctttcccgag ttgctcgaat
     3781 tcttcattct ttgcaacgaa atcagtttcg caattcagct gcagcaatac gccaatcttg
     3841 ccttctccgt gaatgtagga aaacacacgg ccggatccag tgtcgcgatc cattcttttg
     3901 gctgctttcg cgagaccttt cttgcgaaga gcttcgcttg ctttttcgat atcgccggcg
     3961 ttttcagcca gagcagactt gcagtccatc atgcctgcgc cggtcatttc gcgcagtttc
     4021 ttgatgtcgt cggatttaac ttctgccatg atcgtctaat tcctcaggat tcttttggag
     4081 cagcaggtgg agctacatat tctttcacag ctgcttcttc atctggattg gtcttcggag
     4141 aattcgcatc gtccgggatg aattctccgg actcgtcgta ctcaccgcga tattgcagtg
     4201 aatccagagc gatcgcatct ggatccatag ttgcatcttc gtctgtaaag cgagagccct
     4261 gctctactcc ctgcgttcct tctagaaccg catctgccat agtttgcaga tagagagaga
     4321 tggcacggat cgcgtcatcg ttgcctggaa ttggatagtc gatgagttct ggattgcagt
     4381 tagaatccac aacagcaaag atcttgatgc ccatcgcgcg cgcttctttg actgcgatgt
     4441 cttctttgct tggatcaatg acaaaaagga tttccggtgg agactggagc tctttgatac
     4501 cggaaagatg cttgcgcagc ttgtccagtt ctctctgaag caagagcgct tctttctttg
     4561 tgcgcgcttc ttgatcaaaa gatccagccg cttccat
//
LOCUS       DAORFF010000297         4593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10828_length_4593_cov_3.608418, whole genome shotgun sequence.
ACCESSION   DAORFF010000297 DAORFF010000000
VERSION     DAORFF010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10828_length_4593_cov_3.608418"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PKK76_12095"
     CDS             <1..379
                     /locus_tag="PKK76_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69263.1"
                     /translation="QICKMLSGQTPGAPYQPGVWFNSARFFTLDYQAYGTMPKDSKSV
                     LMIDHANQKSIRIACHGATRAVCGFVLMGIRYRHEVCEKWIQEERSLDYVLANLHEAN
                     FDPEFSKKYEPLVRTQAEKAGFI"
     gene            376..1461
                     /locus_tag="PKK76_12100"
     CDS             376..1461
                     /locus_tag="PKK76_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNL69264.1"
                     /translation="MRIPAWIGRITLAVGFTLFYIVIYFGDKLTFAGGPLQTTMQSAV
                     YSLLDGLAFLVRGRPSDQWFFYGTVYTAAVLVFGVRSLIKYRASRYQMIRTGSVMFFQ
                     LFFAYLIPGWLQLFKQPEFYFSYFWPLKPEYFYPYRWAGLAASSPSFWFLFFALFMTV
                     VATPLLTYFFGKRWYCSWVCGCGALAETMGDPFRDLSNKSTAVWKFERWIVHGILAFI
                     TITTVLLWINSATSGAVFGNLSSRLAEWYGFYIGAIFSGVIGVGFYPLLGSRVWCRFG
                     CPMAAYLGILQKYFSRFRITTNGGQCISCGNCSNYCEMGIDVKWYAQRGQDIVRASCV
                     GCGICSEVCPRGVLKLEAVSGARANRH"
     gene            1610..2722
                     /locus_tag="PKK76_12105"
     CDS             1610..2722
                     /locus_tag="PKK76_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNL69265.1"
                     /translation="MCRADLALIFFYRGCTHVVSTATATAVWGQGSAGTSFTSSATGL
                     SSTTINGPTYAYPDMANGLLVIDDVNVRILYFPTNSFTATQVWGLGPPGNNFTSSGAG
                     GTTSTTFDTATALLRDAAGGLYVADSLNNRVLYFPSSSYVATKVWGQGAAGNDFVSST
                     SALSATALNGPGGLALDSTGGLYVSDTGNGRVVYFPPGSYTATRAWGINASGTTLTSG
                     GSGVSAVGLTSPKGIRVDLYGGLYVTDQARIVYYAPGSFTATKVWGQGSGGNSFTTNS
                     ASVSPTGLTNALDLALDDLGGLYAVDATANRVIYFPPQSFTATTVWGQGASGTSLTTN
                     ASGTTAVTLSNPASVALDNSCRVIIADAANNRVLRY"
     gene            complement(2772..2978)
                     /locus_tag="PKK76_12110"
     CDS             complement(2772..2978)
                     /locus_tag="PKK76_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69266.1"
                     /translation="MKFISAVALAISLGAVSLFAVQPYFQCTGKLNGNKVKHAGYYAD
                     AGAAKRAFEAQRKGVTDVSCAPKK"
     gene            complement(3043..3756)
                     /locus_tag="PKK76_12115"
     CDS             complement(3043..3756)
                     /locus_tag="PKK76_12115"
                     /inference="COORDINATES: protein motif:HMM:NF017672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HNL69267.1"
                     /translation="MSCPYQPLLSTYGPPNVKWCEESLCAIVQEPANTWSNLLFIFVG
                     MWILTQARKRRPFVALGLAITIMGTLSLVYHASNLYPTQLGDFLGMFLYLSLLHMWNI
                     RRAGVALTQNASVIVYFLIVYMNIGMLVLFPMIGLRIQLIIFINTLVVFAEEAFLARG
                     VIAEMGRSPYKNYMLGVVFVCAAAVCSALDYSRRWCDPTNHWIQGHAVWHLLSAVSVI
                     FVFRFYNQIGTGANVRAAA"
     gene            3841..>4593
                     /locus_tag="PKK76_12120"
     CDS             3841..>4593
                     /locus_tag="PKK76_12120"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNL69268.1"
                     /translation="MIFRYVAFFLLAVWIFAPLFAQPVEDRTTLKSERPVSFVSAISG
                     LFLRQSPGTDAPTIRLLKYATPLEVLKRKEPFERVGDKEGQWLLVKTEDVTGWVFGAY
                     VTHAIPASPIRHWNASHTMYFVVTSKAPGGDCASAYNYDQPCWISAFDSRGNVMRPYQ
                     GAHSGSVGSAGWEKDEIELFTYGVDGGGGGSSNTLWSPVTGQEHEIFHSYFIPNRNKD
                     GETTSVLFEICFKEKCHFIEQFEKTHTCDVWQG"
BASE COUNT         1120 a         1197 c         1144 g         1132 t
ORIGIN      
        1 acagatctgc aaaatgttgt ccggtcagac gcccggggca ccctatcagc ccggtgtgtg
       61 gttcaattcg gcgcgcttct ttactctgga ctatcaggcg tacggaacta tgcccaaaga
      121 cagcaaatcg gttctcatga tagaccatgc gaatcagaaa tccattcgca tcgcctgcca
      181 cggagcaacc cgggctgtgt gcggatttgt tctgatggga atccgctaca gacatgaggt
      241 gtgcgaaaag tggattcagg aagagcggag tctggactac gtacttgcaa acttgcatga
      301 agctaatttc gatcctgaat tttcaaaaaa atacgaaccc ctggtacgaa cgcaggcaga
      361 aaaggcaggt ttcatttgag aatcccggca tggatcggtc ggatcactct ggctgttgga
      421 tttactcttt tttacatagt aatttatttt ggagataaac tcacttttgc tggcgggcct
      481 ctgcaaacga ccatgcagtc tgctgtctat tccctgctcg acggccttgc cttcctggtt
      541 cgcggaaggc cttcggatca atggtttttc tatggaacgg tttacacagc tgcggtttta
      601 gtttttggag tgcgcagcct catcaaatac agggcttcgc gttaccagat gattcgcacc
      661 ggttcggtca tgttctttca gttgttcttt gcgtatctga tccctggttg gctgcaactt
      721 ttcaagcaac cagagttcta cttcagttat ttctggcctc tgaaaccaga atacttctat
      781 ccctaccggt gggcaggcct cgccgcatcg agtccttcgt tctggtttct atttttcgct
      841 ctcttcatga ctgttgtagc aacacctctt cttacttatt tctttggcaa gagatggtat
      901 tgctcctggg tttgcggatg tggagcactt gcggaaacca tgggagatcc atttcgcgat
      961 ctttccaaca agagcacagc agtctggaaa ttcgaacgct ggattgtgca cggaattctt
     1021 gccttcatca caatcacaac cgttcttttg tggatcaact ccgcaacctc cggagccgtc
     1081 tttggaaacc tttcttcgag actcgctgaa tggtatggat tctacattgg tgctatcttc
     1141 agtggagtca ttggagtagg cttttatcca cttctcggtt cacgcgtctg gtgtagattt
     1201 ggctgcccta tggctgctta ccttgggatt ctgcagaagt atttctccag gtttcgcatc
     1261 actaccaacg gcggtcagtg catcagttgc ggcaactgca gcaactactg cgaaatggga
     1321 atcgacgtga aatggtatgc gcagcgaggc caggacattg tgcgcgcttc ctgcgttggg
     1381 tgtggcatct gttcagaagt ctgtccgcga ggagtgctga agctggaagc tgtatctggc
     1441 gccagagcaa atcggcacta gatcggaaac aattcttcaa aattcactcg ccctcaaacg
     1501 agtttctgcc gtctaaatca tggtgaaggt tagtccctac gctctgttac tgctgctgcc
     1561 cggcctgctt cattgcagat acccgtctca ttgctcttca tcagacatca tgtgccgcgc
     1621 ggatcttgct ctgatcttct tttaccgtgg atgcacgcat gtggtcagca cagccacggc
     1681 aaccgcagtg tggggtcagg gatctgcggg aacgagtttc acgagcagtg ccaccgggct
     1741 ttcttctacg accataaacg gtcccaccta cgcgtatccg gacatggcca atggattgct
     1801 cgtaatagat gacgtcaacg ttcgcatcct gtatttcccc acaaacagct tcacggcaac
     1861 gcaagtctgg ggactcgggc ctcccggtaa taatttcacg tcatctggag cgggaggaac
     1921 gacttccaca acatttgaca cagcgactgc cctgctcaga gacgcggcgg gagggctata
     1981 cgtagcagac agcctcaaca atcgcgtttt gtactttccc tcaagttctt atgtggcaac
     2041 gaaggtgtgg ggtcaggggg cggctggaaa cgactttgtc tcatccacta gcgcactttc
     2101 ggcaactgca ctcaatgggc ccggaggtct ggcactggac tccacgggtg gattgtatgt
     2161 gtccgacaca gggaacggcc gtgtcgttta tttcccgccg ggtagctaca ctgccacacg
     2221 ggcctgggga atcaatgcat ccggaaccac cttaacctct ggaggctcgg gcgtctcggc
     2281 agttggactt acctcaccaa aagggataag agtggatttg tatggcggat tgtatgtgac
     2341 ggaccaggcc cgaatcgtct actacgctcc gggttcgttc actgccacga aagtctgggg
     2401 ccagggctcc ggtgggaata gcttcactac aaatagcgcc agcgtcagcc ccaccggcct
     2461 caccaacgca ctggatcttg ctttggacga tctgggtggt ctatatgctg tcgatgccac
     2521 ggcgaacagg gtgatctact ttccgcccca aagctttacc gcgacaacag tatggggaca
     2581 gggcgcgagc gggacaagct taactaccaa tgcttcggga acgactgccg tcactctgag
     2641 caatcccgcc tctgtggctc tcgacaactc atgccgcgtg atcatcgcag atgcggcaaa
     2701 caatcgagta cttcgttact aggtagcgcg ctcccatgca caggcacggg cgcggcaaga
     2761 aaaatcaaaa tctatttttt gggggcgcag gaaacgtcag tcacgccttt tctttgcgct
     2821 tcgaacgctc ttttggcggc acccgcatct gcatagtatc ctgcgtgctt cactttgttc
     2881 ccgttgagct ttcccgtgca ctgaaagtat ggctgaacgg caaacagcga aactgcgcca
     2941 agggaaattg caagagcaac tgctgatatg aacttcatgt aaactccttt gtttttttag
     3001 atgcgccagg gttcgcctaa cgcttctaaa gtcaaaatat tctcacgcag ctgccctgac
     3061 atttgcgcca gtcccaatct gattataaaa gcgaaagaca aaaatcacgg acacagcgct
     3121 caacaaatgc catactgcgt gcccctggat ccagtgattg gttggatcgc accatctgcg
     3181 agaatagtcc agagcggagc agacagctgc ggcgcaaaca aacaccactc caagcatgta
     3241 attcttgtaa ggcgaacgtc ccatctccgc aatcactccg cgcgcgagaa acgcttcctc
     3301 tgcaaacacg accagggtat tgataaaaat aatcaactga atgcgcaggc caatcatagg
     3361 aaagagaaca agcattccta tattcatgta aacaatcaaa aaatagacga tcaccgaagc
     3421 attctgggtc aacgcaactc ccgcgcggcg gatgttccac atatgaagca agctcagata
     3481 gagaaacatt cccagaaaat caccgagctg cgtaggataa agattcgagg cgtgatacac
     3541 cagagaaagc gttcccatga tggtgatagc cagaccaaga gccacaaaag gcctcctctt
     3601 acgagcttga gtgagaatcc acatgccaac aaaaatgaac aaaaggttgc tccatgtatt
     3661 ggcaggctcc tgcacaatcg cgcacaggct ctcttcgcac cacttcacat tgggagggcc
     3721 gtatgtactg agtaaaggtt gatagggaca gctcatggct caacacgcaa tgcggaacaa
     3781 aaacgtccag aacgaatttc gtttgaccgg cggcaaaata cgtattgtca tcattctgca
     3841 atgatctttc gctatgtggc tttcttcctg cttgcagtat ggatcttcgc gcctctcttt
     3901 gcacaaccag tagaggatcg aaccaccctg aaatcagaac gtcctgtcag cttcgtaagc
     3961 gcaatttccg ggctatttct aaggcaatcc cctggaacgg atgctcccac cattcgtctt
     4021 ttgaaatatg caactccact ggaagttctc aaacgaaagg aaccatttga gcgagtagga
     4081 gacaaagagg gccagtggct tctggtcaag accgaagatg tcacgggatg ggtctttggt
     4141 gcctacgtca ctcacgcgat tcctgcttct cccattcgac actggaatgc atctcatact
     4201 atgtacttcg tggtaacgtc aaaagcaccc ggaggagact gcgcgtccgc atacaactac
     4261 gatcagccgt gttggatttc agcctttgat tccagaggca acgttatgcg accttaccag
     4321 ggagctcact caggttcggt tggatctgca ggatgggaga aagacgaaat tgaactcttc
     4381 acttatggag tcgatggcgg aggtggaggg tcctcaaaca cactgtggtc tcctgtgaca
     4441 ggccaggaac acgagatctt tcacagctac tttattccaa acagaaacaa agatggcgag
     4501 acaacttccg ttctattcga aatctgcttc aaagaaaaat gtcatttcat agaacaattt
     4561 gaaaagactc acacatgcga cgtttggcag gga
//
LOCUS       DAORFF010000298         4579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10873_length_4579_cov_4.204244, whole genome shotgun sequence.
ACCESSION   DAORFF010000298 DAORFF010000000
VERSION     DAORFF010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10873_length_4579_cov_4.204244"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1128
                     /locus_tag="PKK76_12125"
     CDS             121..1128
                     /locus_tag="PKK76_12125"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL69269.1"
                     /translation="MKGLASIWFLLVLAGCHRDDCLVYDGECNPFVTALLFSRVQAAR
                     FIAVSNSTGGSVSMYQMNYSTGTLLPVSGSPFAAGASPHHLAAHPSGRFLYLANNGSN
                     NISGYAVNSNTGALTQVSGSPFASVTAPYSVTIDPTGTYLYAGSETTAFISAYQIDSS
                     TGVLTQISGSPFGASPVAGSVRVSPSGRFLYVGVNNATGQVLAYAIASNGSLTTISGS
                     PFASGNDTISVTVDPYERFVLGINYFGDATGLVALSAYFIGASGAMIPLGAGPYAAGA
                     SPIGCAAEPLGRYVFSANTGSNTVTMHTINQTSGVPTIVGTYSTGNGPFGVSFIPTLQ
                     Y"
     gene            complement(1125..2501)
                     /locus_tag="PKK76_12130"
     CDS             complement(1125..2501)
                     /locus_tag="PKK76_12130"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNL69270.1"
                     /translation="MKGLLTLDQLKEKHAKGEIETVLVAFPDQYGRFMGKRYTASFFL
                     DAVARDGMHACDYILTVDMEMDVIPGYKLSSWEKGYGDFHCKPDMSTLRQLTWLDRTA
                     LVICDLVNEKTHALVGPSPRAMVQHQIDRLRAAGYAGLGASELEYFMFDETYESAAAK
                     NYENLKTFGNYIEDYHIFQGTKAERLNAAIRKHMDDSAVPVESSKGEWGPGQQELNFR
                     YCDVLGMCDRHTIYKQGIKEIAWAQNLAVTFMAKWKTEYAGSSMHLHFSLWDKDIKKN
                     VFAGDKKFGPVMCSDEFRWFLGGWMQHIRAIMPFYAPYPTSYKRYLSQSWAPVYIAWS
                     HDNRTAGFRVVGSGNSLRIEARFPGADANPYLAFAASIAAGLDGLKNKIEPPQIFEGD
                     VYSARELPRVPSNLMEAVSHLEKSEMLKEAFGADVVEHYVHFFKVEQEKFDQQVTTWE
                     RGRYFERA"
     gene            complement(2504..3160)
                     /locus_tag="PKK76_12135"
     CDS             complement(2504..3160)
                     /locus_tag="PKK76_12135"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNL69271.1"
                     /translation="MMQTLHNLVIVWFGLVHDWGYGGIVFLMALESSIVPIPSEVVMP
                     PAAFWAAQGKMNFWFVVLAGTVGSYLGSVISYFVARSIGSPLLYRFGKFIFLPPEKLK
                     LAEAWFNTYGAAGIFFARLLPVIRHLISIPAGVFKMNFAAFSTWTIVGAGLWCIVLSW
                     YGGQVLGESPQLLESPIEMVEVLKAKMYWIIAGVAGIAILYGIMVFAKRRLLEKKPEA
                     "
     gene            3276..4088
                     /locus_tag="PKK76_12140"
     CDS             3276..4088
                     /locus_tag="PKK76_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69272.1"
                     /translation="MGGKLNTLRVVLVESRATFLRAFRHTREDWRTLVRATGIFSACL
                     AVVYTIGVFAVMRFTYSLAFTAPEYIRELQNILLNVIAMPFLAYFLGGLLQFFRERTR
                     GTQAPYILLIKGHRHYFRILLYCLAYYAGYRAFVRTVVGIEDYPVDLRLRMLFGLPLF
                     IWVITRVCFSFAFVTEQDAGPKLGIKSSFILTGGRFWRTLVILFPVPVSFATAGLCIG
                     LLPHPVLVILALILVCAALPFVLSVVFLSYFWLFDRYRQEPTVVEIAAVTGQ"
     gene            complement(4097..>4579)
                     /locus_tag="PKK76_12145"
     CDS             complement(4097..>4579)
                     /locus_tag="PKK76_12145"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNL69273.1"
                     /translation="MGMDWRLLAAIAYQESRFDPTARSWAGAQGVMQLMQPTAVQFGA
                     PNAFVAEANIKAGAKYLAWLDEQWKSITDPDERIKFVLASYNAGPAHVEDARRLATRY
                     KKDPNKWFDHVEIFILDLSRPEFYNDPVVQYGYLRGEEPYHYVREILERYEHYKKFVK
                     "
BASE COUNT          959 a         1193 c         1118 g         1309 t
ORIGIN      
        1 tggacggaaa tttcgtggac tcccctttta ttttaagggt ttttaggtct tcggcgggtt
       61 atgcgatgga ttctggttgt ttcctgcgcg tagtgcgttt ctttgtatgg gttccccatc
      121 atgaaaggct tagcatccat ctggtttctg ttggttctgg ccggttgtca tcgcgatgat
      181 tgtctggtct atgatggcga atgcaatccc ttcgtaaccg ccttgttatt ttcgcgtgtt
      241 caggcggcca ggttcatcgc cgtctcgaac agtacgggcg gaagcgtttc gatgtatcaa
      301 atgaactact ccactggaac gctcttgcct gtctccggat ctccgtttgc tgcaggtgcg
      361 agccctcatc atctagcggc ccatccttcg ggccgatttt tgtatcttgc aaacaatggt
      421 tccaataaca tatccgggta cgctgtgaat tcgaacacgg gtgctctgac acaggtttct
      481 ggttctcctt ttgcttccgt gactgctccc tattctgtta cgattgatcc aacaggaacc
      541 tacctttatg caggatcgga aaccactgcg tttatttccg cctatcagat tgatagttct
      601 actggagtct tgactcagat ttccggatct ccttttggtg ccagtcccgt tgccggatct
      661 gtgcgtgttt ctccatccgg tcgtttcctt tacgttggag tcaacaatgc aacaggccag
      721 gttctggctt acgcaatagc gtctaacggt tccctgacca cgatttccgg atcccctttt
      781 gcttctggca atgatacgat ttcggtcacg gtagatccgt acgaaagatt tgttttagga
      841 attaactatt ttggagacgc cacaggactc gtggctttgt ccgcgtattt tattggtgcg
      901 agcggggcca tgattccgct gggcgctggc ccctatgcag ctggagcaag cccgattggc
      961 tgtgccgccg aaccactcgg tcgatacgtc ttttctgcaa acaccggctc aaacactgta
     1021 accatgcaca cgatcaacca gacgagtgga gttcccacca ttgtagggac ttactccact
     1081 ggaaacggtc cattcggtgt ctcctttatt ccaaccctgc agtactaggc gcgctcgaag
     1141 tatcttcctc gttcccaggt ggttacttgc tgatcaaatt tctcctgttc tactttgaag
     1201 aaatgaacgt agtgttccac cacgtctgcc ccgaatgctt ccttgagcat ttcagatttc
     1261 tcgaggtgcg aaactgcttc cattaaattg gaaggtacac gtgggagttc tcgcgctgag
     1321 taaacatctc cttcaaagat ttgcggaggt tctattttgt ttttgagtcc atccaggcct
     1381 gctgcgatgg atgcagcaaa cgcaagatat ggatttgcat cggcacctgg aaatcttgct
     1441 tctattctga gagaatttcc tgatcccact acgcgaaagc ctgcagtgcg attgtcgtga
     1501 ctccatgcaa tgtaaacggg tgcccaggac tgagatagat atcttttgta agaagttgga
     1561 tagggcgcgt agaagggcat gatggcgcgg atatgctgca tccaaccgcc cagaaaccag
     1621 cgaaattcgt cagagcacat cacaggtccg aatttcttat ctcccgcgaa aacgttcttc
     1681 tttatgtctt tgtcccacag cgaaaaatga aggtgcatgc tggacccggc atattccgtt
     1741 ttccattttg ccatgaaggt tactgccagg ttctgcgccc aggcgatctc tttgattccc
     1801 tgtttgtaaa ttgtgtggcg atcgcacatg cccagtacgt cgcaatatct gaagttcagt
     1861 tcctgctgcc cggggcccca ttctcccttg ctggattcta cgggcacggc gctgtcatcc
     1921 atgtgcttgc gaatggcagc attcaatctc tccgctttgg ttccctggaa aatgtggtaa
     1981 tcttctatgt aattgccaaa tgttttgaga ttttcgtaat tttttgcggc cgcactttca
     2041 tacgtttcat caaacatgaa gtattccagt tcgcttgcac cgaggcccgc atatccagcc
     2101 gcgcgaagtc tgtctatttg atgctgcacc atggccctgg gcgatggacc tacaagcgca
     2161 tgagtctttt cattcaccag atcgcaaatc acaagtgctg tgcgatccag ccacgtaagt
     2221 tggcgaagag tgctcatatc tggcttgcag tggaagtctc catacccttt ttcccagctc
     2281 gacaatttgt atcctgggat tacgtccatt tccatgtcta cggtcagaat gtaatcgcaa
     2341 gcatgcattc catcacgagc cactgcatcc aggaagaacg atgccgtata acgttttccc
     2401 atgaagcgac cgtactggtc agggaaagcg accagaaccg tttcgatttc gccttttgcg
     2461 tgtttttctt tcagctggtc tagagtgagc aatcccttca tagttatgcc tccggctttt
     2521 tctcgagcaa acgtctcttc gcaaacacca tgatgccgta cagaattgca atccctgcaa
     2581 ctccggcaat gatccagtac atcttcgctt tgaggacttc taccatttct atgggtgatt
     2641 cgaggagctg tggactttcc ccgagaacct ggccaccgta ccagctcagc acgatgcacc
     2701 acaggccggc acccacaatg gtccaggtgg agaacgcggc gaagttcatt ttgaatacgc
     2761 ccgccgggat agaaatcaaa tgccgaataa cgggaagcaa gcgtgcgaag aagatgccag
     2821 ccgctccgta tgtattgaac catgcttccg caagtttcag tttttcaggt ggcagaaaga
     2881 taaattttcc aaaacgatag agaagcggac ttccaatgga ccgggcaaca aagtaactga
     2941 taacacttcc caggtagctt ccgactgttc cggcgagcac aacgaaccag aaattcatct
     3001 taccctgtgc ggcccagaag gcggcgggtg gcataacgac ctcagaagga ataggcacga
     3061 tagagctttc cagggccatc aggaagacga ttcctccgta gccccagtca tgcaccagac
     3121 cgaaccagac gatgacgaga ttgtgcagag tttgcatcat agagttcgtg catttcgcat
     3181 tctgccgtaa ccactggcta gcgaattttt gcagaggagc ccggccgcag tctgaatgat
     3241 gttgactctt tccagggccc gcagacaatt cgggcatggg gggaaagctc aatactttgc
     3301 gcgtcgtcct tgtggagagt cgcgcaactt tccttcgtgc gtttcgtcat acccgcgaag
     3361 actggcggac tctcgtgcgc gccacaggaa ttttcagtgc ctgcctggcc gtcgtctata
     3421 ccatcggtgt gtttgctgtg atgcgattca cctattctct tgcctttact gctccagagt
     3481 acatccgcga attgcagaac attttgctca atgtcatcgc catgcctttt cttgcctatt
     3541 ttctcggagg gctactgcag ttctttcgtg agcgaaccag aggcacccag gcgccttaca
     3601 ttttactcat caagggtcac aggcattact ttagaatcct attgtattgc ctggcgtact
     3661 atgctggata tcgcgcgttc gttcgcacgg tggttggaat cgaggattac cctgtggatc
     3721 tcaggctcag gatgctgttt ggattgcctc tcttcatctg ggtcatcact cgcgtgtgtt
     3781 tctcttttgc gttcgtaaca gaacaggatg caggtcccaa gcttggaatt aagtcttctt
     3841 ttattttaac gggggggcgg ttctggcgga ctctcgtgat cttgtttcct gttcctgtga
     3901 gttttgccac cgccggcttg tgcattggcc tcttgcctca tccggtactt gtgattctgg
     3961 ctttgattct ggtgtgcgcg gccctgccct tcgtgctctc tgtcgtattt ttgtcctatt
     4021 tctggctctt tgatagatac aggcaggagc caacggtcgt ggagattgcc gcagtcacag
     4081 gccagtaata gttaatttat tttacaaatt tcttgtagtg ttcgtatctc tccaatattt
     4141 ctctcacata gtgatacggt tcttctcctc tcagatatcc atactgcacc accggatcgt
     4201 tgtaaaactc ggggcgtgaa agatccagga tgaagatctc cacatggtca aaccacttgt
     4261 ttggatcctt tttgtagcga gtggccagtc ttctggcgtc ttccacgtga gcggggcccg
     4321 cattgtacga ggccaataca aatttgatgc gttcgtccgg atctgtgatg gatttccatt
     4381 gttcatcgag ccaggccagg tatttcgcgc ctgctttgat gtttgcctct gcaacaaaag
     4441 cattgggagc cccaaattgc actgctgtcg gctgcatgag ctgcatcaca ccctgtgcgc
     4501 ctgcccacga tcttgcagtc ggatcgaatc tggattcctg gtaggcaatc gcggcgagga
     4561 gcctccagtc cattcccat
//
LOCUS       DAORFF010000299         4579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10874_length_4579_cov_3.722812, whole genome shotgun sequence.
ACCESSION   DAORFF010000299 DAORFF010000000
VERSION     DAORFF010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10874_length_4579_cov_3.722812"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PKK76_12150"
     CDS             complement(<1..763)
                     /locus_tag="PKK76_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69274.1"
                     /translation="MYVSECFQNLAIEERTRLEKRLGIILRRVEERHQIRKTLRNIFE
                     SMEVRLRAEFEAGAIWTGLLDHFCILPQDRMTGEAAEALKKHPLTFWPESHICTMSAE
                     AMEVVGRMDCVRESRSILSELLRMGTPDKRALSRWLGLDAPASALDLYSLIGQTRGSE
                     SPDFSEFNHRYLTDLFPEDAMALLWFYRGVLPLYECLRLAETENIQAEQKKCLEGFRL
                     GYLIAEPLPVEFGQTQKFKIVCTREKNAGMVSDLPS"
     gene            complement(773..2038)
                     /gene="purD"
                     /locus_tag="PKK76_12155"
     CDS             complement(773..2038)
                     /gene="purD"
                     /locus_tag="PKK76_12155"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNL69275.1"
                     /translation="MNVLLIGSGGREHALFWKLSQSTQLGSIRVFPGNGGFAPESIAL
                     GIDLKNFETIRSYVKQNKIDLVVVGPEQPLVDGITDALSRDCLVFGPTAFCAQLEGSK
                     DFSKAFMKRHGIPTADAVTFTDSNAAIEYLRTRKLPIVVKADGLAAGKGVTVATSYED
                     AETAVRDAIDKKIFGTSGQSVLIEDFLEGQEASVFAICDGQRALPMIAAQDHKRVFDN
                     DQGPNTGGMGAYAPVPFMNADVMRQVQTEVLDRVMAGMQKEGHPYRGLLYAGLMVHNG
                     SVRVVEFNCRFGDPETQGLLRLLDEDLLELCKLSASGNLPERQIHFKQEAVICIVLAA
                     EGYPGEVRKNIPIPDLKIIDSLGDIIMFHAGTALTPGGVVSTGGRVLNLTATGRDLLE
                     ARKLAYSALERVHVPGLFYRKDIGQKGIP"
     gene            complement(2040..2927)
                     /locus_tag="PKK76_12160"
     CDS             complement(2040..2927)
                     /locus_tag="PKK76_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL69276.1"
                     /translation="MNAAAWKEGGKYFRHNGHNIFYRLDGSGEAVIFLHGFPTSSWDW
                     EKIWPALTSQFLCVAPDFIGYGYSDKPHEYNYSILDQADLAENLLRELRIEKAHIFAH
                     DYGVSIAQEMLARSQDRKRERIKGGLEILSVCFLNGGIFPEAHRPRLIQQLLLSPVGR
                     WVSMFINEGAFRASFCEIFGKGTQPAEEDLKQFWELIRLNKGHEIYYRLISYIPEREK
                     NRERWVNAIMNSRVPLRLINGPEDPVSGKHMADQYKKLIQKPDVVLLPGIGHYPQYEN
                     PDAVIANFLEFVRRKEEEE"
     gene            complement(3008..3343)
                     /locus_tag="PKK76_12165"
     CDS             complement(3008..3343)
                     /locus_tag="PKK76_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNL69277.1"
                     /translation="MTTVLLLIASNVFMTFAWYGHLKFRHIPIWAAIFASWGIAFFEY
                     CLQVPANRLGYGQFNAFQLKIIQEIVTLVVFIVFAIFYLGESPRWNHLASLLCIVMAV
                     YFAFLSAPA"
     gene            complement(3345..>4579)
                     /locus_tag="PKK76_12170"
     CDS             complement(3345..>4579)
                     /locus_tag="PKK76_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL69278.1"
                     /translation="IILNGYGLLVGDQNAHALRELQNAAEQANRAKSIFLANMSHEIR
                     TPLSSIIGMSELLLDTELTSDQRERIKVIRLSADTQLAVINEILDYSRLEARKVHIDP
                     HVCDLTQLLSDTLAIIRPMADKKEIGVVAENDPNLPRYVVCDGPKLKQVLLNLLVNAV
                     KFTANGFVALRVDLEERSNTHVRLLFSCKDTGIGLNHADKARIFKPFQQADNTISRQF
                     GGTGLGLAICKQLVGLMGGALDVDSEPGSGSRFFFRLQFPLAGKAEIESHHMRELRPE
                     AHLINKHIRILVADDNDVNRGIMQMMLDRIGYSSETCINGQEAVDKASRNSYAIILMD
                     MQMPEMDGITATKILRARGHKAKIIALTANASAEDRLLCEEAGMDGFLTKPVSMQELS
                     TLADQYLKAVSLSIRPIL"
BASE COUNT         1037 a         1154 c         1149 g         1239 t
ORIGIN      
        1 gggaaggcag gtccgatacc atgccagcat ttttttctct ggtgcacacg atcttgaact
       61 tctgagtctg gccaaattcc actggcagtg gctctgcgat cagatacccc aggcgaaatc
      121 cttcgaggca tttcttttgt tctgcctgga tgttttctgt ttcagcgagt ctcaggcatt
      181 cgtaaagagg caatactccc ctgtagaacc acaaaagggc catggcatct tcggggaaca
      241 ggtcggtgag gtaccgatga ttgaattcgg aaaagtctgg tgactcgctt cctcttgtct
      301 ggccaatgag agagtataga tcgagtgcgg aggcaggagc gtccaggccg agccatcgcg
      361 agagtgcgcg cttgtccggt gtgcccatgc gcaatagttc tgacaggatg gatctgcttt
      421 cgcgaacgca atccattcta cccactactt ccatggcttc tgcagacatg gtgcagatgt
      481 gcgattctgg ccagaaagtg agaggatgtt tcttcagagc ttccgccgct tctccagtca
      541 ttctatcctg gggcagaatg cagaaatggt cgagcaatcc ggtccagatg gcacctgctt
      601 cgaattcggc acgaaggcgc acttccatgg attcaaaaat attgcgcagc gtcttacgga
      661 tctggtgtct ttcttctact cgcctgagaa tgatgccgag tcttttttca aggcgagttc
      721 tttcttctat cgcaaggttc tgaaagcatt cggaaacgta catcgcgacc tctcagggaa
      781 ttcccttctg tcctatgtcc tttcggtaga agaggcctgg aacgtgaaca cgctccaggg
      841 cactgtatgc aagcttccgc gcttcgagca aatcacggcc cgttgcagtc agattcaaca
      901 ctcttccccc ggtggaaacg actccgcccg gtgtaagggc agtgccagcg tggaacatga
      961 ttatgtctcc taacgagtca atgattttga gatccgggat gggtatattt ttccgcactt
     1021 caccggggta accctctgct gcgagcacta tacagatgac ggcttcctgt ttgaaatgaa
     1081 tttgtctttc cggaagattt ccagaagcac tcagcttaca cagttcaagc aaatcttcat
     1141 ccagcaatct gagaagcccc tgcgtttctg gatcaccaaa tctgcagttg aactcaacta
     1201 ctcgcacaga cccgttatgc accatgaggc cagcatacaa aagtccgcga tacggatgac
     1261 cttctttctg cattcccgcc atcacgcgat cgagcacttc cgtctgtact tgtcgcataa
     1321 cgtctgcgtt catgaaaggc acaggagcat acgcgcccat tccgcctgtg ttcgggccct
     1381 gatcattgtc gaatactctc ttgtggtcct gcgctgcgat catgggtaga gctctttgtc
     1441 catcacagat tgcgaagaca ctcgcttcct gaccttctag aaagtcttct atgagtacgg
     1501 actgaccgga tgttccgaat atctttttgt cgattgcgtc tcttacggct gtttctgcat
     1561 cttcgtagct cgtggccacc gttacgcctt tgcctgcagc cagaccatcc gctttcacta
     1621 cgataggtag ctttcgcgtg cgcagatact ctatggctgc atttgagtct gtgaatgtca
     1681 ccgcatccgc agtcgggatg ccgtgtcttt tcatgaacgc ttttgaaaaa tctttagatc
     1741 cctctaattg cgcgcagaaa gccgttggtc cgaataccag gcaatctctt gatagagcgt
     1801 ctgtgattcc gtctaccagt ggttgttcgg gtccaactac caccagatcg attttattct
     1861 gttttacata agatcggatg gtttcaaaat tcttgagatc gatacccaga gcgatgctct
     1921 ctggtgcaaa cccaccgttg cctggaaaaa cccggatgga tccgagctgc gtgctctgag
     1981 aaagcttcca gaagagcgcg tgttctcgcc cgcctgagcc aatcagaagt acgttcatac
     2041 tattcctctt cttctttgcg ccggacgaat tccaggaaat tcgcgatcac tgcgtccggg
     2101 ttttcgtatt gcggatagtg gcctattcct ggcaagagaa ccacgtccgg tttttgaatc
     2161 agtttcttgt attgatccgc catgtgtttg ccagagactg gatcttctgg cccgttgatg
     2221 agtctgagcg gaacacgtga gttcatgatc gcatttaccc aacgctctct gttcttttct
     2281 ctttctggaa tgtaagaaat caaacgatag tagatttcat gccctttgtt caggcgaatg
     2341 agttcccaga attgcttgag atcttcttca gcgggctgag tgcctttccc gaagatttca
     2401 cagaacgacg cgcgaaaggc gccttcattg atgaacatgg aaacccatct tccaacgggg
     2461 ctgagaagaa gttgttggat gagtcttggc ctgtgtgctt ctggaaaaat tccgccgttc
     2521 aagaagcaaa ctgaaagtat ttcaagtccg cccttgattc tttctctttt gcgatcctgg
     2581 gaacgagcca gcatttcctg ggcgatgctc acgccgtaat cgtgtgcgaa gatgtgcgct
     2641 ttttctattc gcagctctcg cagtagattt tcagcgagat ctgcctggtc tagaatggag
     2701 tagttgtatt cgtgaggttt gtctgagtag ccatagccaa tgaaatccgg agccacgcaa
     2761 agaaactgag aagtgagagc gggccagatt ttttcccagt cccacgaaga agtagggaag
     2821 ccgtgcagaa agataactgc ttctccgctt ccgtccaggc ggtaaaaaat attatgtcca
     2881 ttgtgacgaa aatatttccc accttccttc caggctgcgg cattcattca ccccaggctg
     2941 tggaatcgct cgtacaggga cagaattttt tttagatcag actccttccg gatgcctgcg
     3001 atctttttta cgccggagca gatagaaatg cgaagtacac tgccatcaca atgcacagca
     3061 aagacgccag atgattccat cgcggacttt ccccgaggta gaagattgca aatacaatga
     3121 aaacaaccag agttactatc tcctggatga tcttgagctg gaaggcattg aactggccat
     3181 agcccagtct gtttgccgga acctgaaggc aatattcgaa gaatgcaatt ccccagctgg
     3241 cgaagatcgc agcccagatg ggaatgtgcc tgaatttgag gtgaccatac caggcgaagg
     3301 tcatgaacac attgctcgcg atgagcaaca gtactgtagt catactagag tatcggacgg
     3361 atcgacagac tcactgcttt tagatactga tcggccaatg tggatagctc ttgcattgat
     3421 acaggttttg taagaaaacc atccataccg gcttcctcgc acaagaggcg atcttccgcg
     3481 ctcgcattcg cggtcagcgc gatgatcttc gctttgtgac ctcgagcgcg caggatcttt
     3541 gtcgcggtaa tgccgtccat ttcaggcatt tgcatgtcca tcagtatgat tgcgtatgag
     3601 ttcctggatg ccttgtcgac tgcttcctgg ccgttgatgc atgtttcgga cgaataaccg
     3661 atacgatcga gcatcatttg catgattcca cgatttacgt cgttatcgtc ggctacaagg
     3721 atgcggatat gcttattgat caggtgagct tccggtcgca gctctcgcat gtgatgtgac
     3781 tcgatttctg ctttgccggc cagcgggaat tgcaacctga aaaagaagcg ggaacctgag
     3841 ccaggttcgg aatcgacgtc cagggcgcca cccataaggc cgacgagttg cttgcaaatc
     3901 gcgaggccca ggcccgttcc accaaattgc ctcgatattg tgttgtctgc ttgctgaaaa
     3961 ggtttgaaga tgcgggcctt gtccgcgtga ttgagaccga ttccggtgtc tttgcagctg
     4021 aacagcagtc tgacatgcgt gtttgagcgt tcttccaggt ccacgcgcaa tgcaacaaac
     4081 ccgtttgctg taaacttgac cgcgttgacc aggaggttca gcaatacttg tttgagcttt
     4141 gggccgtcgc agaccacgta acgtggtaga ttcggatcgt tttccgcgac gacaccaatt
     4201 tcttttttgt ctgccattgg tctgatgatt gccagtgtat cagacagcaa ctgtgttaga
     4261 tcgcagacgt gcggatctat gtgtaccttg cgcgcttcga gtctggaata atctagaatt
     4321 tcattaataa ctgccagttg cgtatcagcg ctaagtctga tgactttaat gcgctcgcgc
     4381 tggtcggagg tgagttcagt atctaacaag agttcgctca ttccgatgat agaacttaga
     4441 ggtgtgcgaa tctcatggct catgttggcc aggaaaatgg atttcgcacg attggcttgc
     4501 tccgcagcgt tttgtagctc gcgaagggcg tgtgcgtttt ggtctcctac cagcaagccg
     4561 tatccgttca ggatgatca
//
LOCUS       DAORFF010000300         4563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10930_length_4563_cov_3.265528, whole genome shotgun sequence.
ACCESSION   DAORFF010000300 DAORFF010000000
VERSION     DAORFF010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10930_length_4563_cov_3.265528"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..2354
                     /locus_tag="PKK76_12175"
     CDS             72..2354
                     /locus_tag="PKK76_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL69279.1"
                     /translation="MLSATLLKTTLELLRDHLGTTGISYRAGGQVIYESGRIAGGGPV
                     AFMNGELFTGLFDKELSANERGAIRTAARMLEVFQGAAIPARGPNAISASLIAKSTFE
                     IAVSLEARSRQLAHYDKILQLNKSILLAQDLQGVLQILMDTAREALTGVGSSLLLVDP
                     RTGELYFNVVSGEKGGELKEIRIPPGKGIAGDVVQKAKAELIRDAVTDPRMFQEVDQK
                     LDQRTRDMVVAPLVARDRVIGVVEVINSQSPEGFTKEDLEFLVNIASHTSLLIDNAKN
                     RDDLIKSNHALDRKISELNAFSEVSRVLNSTLDSIEMRKGLLRTLLKLMHIGQGAIVI
                     PDPTGRRAVSEVRMRLKDGRLEEYGELTVYEDTADILLWMKQNREPLFFANSQGGETE
                     GLVRRFLSANASLEGEHLDLWFPVFQDDQQTVSFIVSLSDVSFRRKHPSDDLTFFKGI
                     MSLSYAAVRNVESYQNVIKAQEREERIRRGFQRYVPERVVEEVFAQEESPAPRSQKVS
                     VLFADIRNFTRHAENRDPGELLDLLNEFFEEMVEAVTHAGGTVDKFMGDSIMVLFGVP
                     DPTDVDAKSALTCARDMIGRLDVLNRRREESSRPTFQVGIGVHTGSAIIGNMGARKRM
                     DFTAVGDTINLGARLEQLTKLYQVRALFSEDTLKEAGDVTCREVDLIQVRGRMAVTRI
                     YQLATNREEDEMIRASAEVWPEMLKAYRMRNFWDCLQILISLPNDPLAGVFRARCESF
                     VKNPPPADWKGFYHLDEAWI"
     gene            2427..2936
                     /locus_tag="PKK76_12180"
     CDS             2427..2936
                     /locus_tag="PKK76_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HNL69280.1"
                     /translation="MKKINIEQTFDHPLDVLLSARQERYKHLDKFPELKNVQIVSETT
                     EGHILKQVRNISLADAMPAVLQTVLPSGANMLVEESEFDDTTNTHTFKVTPGGGLDNI
                     FMVKGVSRYFASGDNKSTRTYDIEVTSKVLFVGGVVETAIGDVYRHNLEKDKSSIEHF
                     IQMLKEGKT"
     gene            2945..3787
                     /gene="argB"
                     /locus_tag="PKK76_12185"
     CDS             2945..3787
                     /gene="argB"
                     /locus_tag="PKK76_12185"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNL69281.1"
                     /translation="MEKELRARVLLEALPYIREFSGKTVVIKYGGAALDDQASLRAQF
                     AEDIVLLHYVGIKPVIIHGGGPRINSLLKMMGIQSQFSGGVRVTDDATMEAVEMVLSA
                     SINKEIVSLIQSKGGRAVGISGRDGNLAHAKKTKPELGRVGEVTPSDIDPAVIRTLLH
                     GGYIPVIAPVASDENRDSLNVNADTMAGAIASALGAEKFVLLTDTPGVLSKGETVPRL
                     SMDSARAMIQSGEISGGMIPKVQCCMSAFEQGVKQIHIVDGRVPHAIILEIFTRQGVG
                     TMIQ"
     gene            3801..4343
                     /locus_tag="PKK76_12190"
     CDS             3801..4343
                     /locus_tag="PKK76_12190"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNL69282.1"
                     /translation="MTKAEKLVKEVVRTVPDWPEKGVMFRDITPIFQHPEVLHAVFEA
                     FIERYREKQVDVIAGIDARGFLLGVTISYELKIPFVPVRKRGKLPWKTVWEEYTLEYG
                     TAAIEMHEDACKKGDRVVIFDDLIATGGTMLAAASIVKRLGGEVVEAAAIIDLPDLGG
                     SQKIWKTGVPVHTLSKYEGH"
     gene            4364..>4563
                     /locus_tag="PKK76_12195"
     CDS             4364..>4563
                     /locus_tag="PKK76_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69283.1"
                     /translation="MALLLAAPVSIFARLPAYADVQKKLPDLWAARFPVPLQSSASDP
                     GKRGMLYCLIDGRPAYYFNFAAT"
BASE COUNT         1173 a         1100 c         1209 g         1081 t
ORIGIN      
        1 agcggaaagg tccgggtcaa gccctttttt ttggcgaggg aagcactctg agatccgtta
       61 attgaagcag tatgctttcg gcaacactgc tcaagacgac cctcgaacta ttgcgcgatc
      121 atctgggaac caccggcatt tcgtatcgcg ctggcggaca ggtgatttat gaatctggca
      181 gaatcgccgg tggcggaccg gttgccttca tgaacggtga gctcttcacc ggcctgtttg
      241 acaaggagtt gtccgcaaat gagcgcggcg cgattcgcac agcagcacga atgctcgaag
      301 tctttcaagg agccgcgatt cccgcgcgcg gccccaatgc aatctctgct tctctcattg
      361 ctaaaagtac gtttgagatt gccgtttcgc ttgaagcgcg gtccagacag ctcgcgcact
      421 atgataaaat tcttcaacta aacaagtcca tccttcttgc tcaggatctg cagggagtgc
      481 ttcagattct gatggatact gcgcgcgaag cgctgaccgg agtgggatct tcccttttgc
      541 ttgtggaccc gcgcaccgga gaactgtact tcaatgtagt atccggcgag aaaggtggtg
      601 aactcaaaga gattcgtatt ccgccgggca aagggattgc gggtgatgta gtccagaagg
      661 caaaagcaga attgatccgc gatgcagtta cggacccgcg tatgttccag gaagtggatc
      721 agaaattgga tcagagaacc agggacatgg tggtcgcacc gcttgtggcc cgcgaccgcg
      781 tcattggcgt ggtagaagtg atcaacagtc agtctccaga aggtttcaca aaggaagatc
      841 ttgaatttct cgtgaacatc gcatcgcata catcccttct gattgacaat gcgaaaaaca
      901 gagacgatct gattaaatcc aatcacgccc tcgatcgtaa aatttctgaa ctaaatgcat
      961 tcagcgaagt cagtcgcgta ctgaactcta ccctcgattc cattgaaatg agaaaagggc
     1021 tccttcgcac cctgctcaaa ctcatgcata ttggccaggg cgccattgtc attccggatc
     1081 ccacaggaag acgtgctgtt tctgaagttc gcatgaggct caaagacggc agactcgaag
     1141 aatatggtga acttacagtc tatgaagaca cagcggatat actgctgtgg atgaagcaaa
     1201 atcgcgagcc tttgttcttt gccaacagcc agggtggaga aacagaagga ctcgtgcggc
     1261 gtttcctgtc cgcaaacgcc tctctcgagg gagagcattt ggatttatgg tttcctgttt
     1321 ttcaggatga tcagcaaacc gtttctttca ttgtatcttt gtctgatgta agctttcgtc
     1381 gcaaacatcc atcggatgat ctcactttct tcaaaggaat catgagtcta tcgtatgccg
     1441 cagtgcgaaa tgtggaatcc tatcagaacg taatcaaggc tcaggaacgc gaagaaagga
     1501 ttcgccgggg attccagcga tacgtaccgg aaagagttgt ggaagaggta ttcgcgcagg
     1561 aagaatcacc cgcaccgcgc agccagaaag taagtgttct atttgcggac attcgcaact
     1621 ttaccaggca tgcggaaaat cgtgatccag gagagctcct ggacctgctc aatgagttct
     1681 ttgaggaaat ggtcgaagcg gtcactcatg caggtggcac cgtggacaag ttcatgggcg
     1741 attcgatcat ggtgctgttc ggtgtacctg atccaacgga tgtagatgcg aaaagcgcac
     1801 tcacctgtgc ccgggatatg attggcagac tggacgtgtt gaatagaaga cgcgaagagt
     1861 cctcaagacc aactttccag gttgggattg gcgttcatac aggttcagcg atcattggaa
     1921 acatgggtgc gcgaaagaga atggatttca cagcagtggg agatacaatc aatctgggcg
     1981 caagactcga acaattgacc aagctctacc aggttcgcgc gcttttctct gaagacactc
     2041 tgaaagaagc aggcgatgtc acctgtcgcg aagtcgacct gatccaggtt cgaggcagaa
     2101 tggcagtcac gcgcatctat cagcttgcga caaaccgcga agaagacgaa atgatccggg
     2161 cctctgcgga ggtctggcca gaaatgctca aggcctatcg catgcgcaac ttctgggact
     2221 gcctccagat cctgatttca ttgcccaacg atccgctggc aggcgtattc agggccaggt
     2281 gcgaatcttt cgtaaagaat ccgccgccag ccgattggaa aggcttctac cacctggacg
     2341 aagcctggat ctgattaacc tggaattctt ctgaattcaa gttgatctct ggacgattcc
     2401 cccacaatgg ggcgcaggat tcctccatga aaaagatcaa cattgagcaa acattcgatc
     2461 atcccctgga cgtgcttctt tcggcgcgtc aggagagata caagcatctt gataagttcc
     2521 ctgaattgaa gaacgtccaa atcgtaagcg agacaacaga aggacacatt cttaagcagg
     2581 ttcgtaacat ttcccttgca gacgcaatgc ctgccgtcct tcaaactgtt ctcccttcag
     2641 gcgccaacat gcttgtggaa gaatctgaat ttgatgatac cacaaacaca cacaccttca
     2701 aagtaactcc gggtggcgga ctcgataaca tcttcatggt aaagggagtc tccagatact
     2761 tcgccagcgg agataacaag tctacacgta cttacgacat cgaagttaca agcaaagtgc
     2821 tcttcgttgg tggagtagta gaaacagcaa tcggggatgt gtatcgtcac aatctagaaa
     2881 aagacaaatc gtccattgag catttcatcc agatgctcaa agagggtaaa acctgagcgg
     2941 tgagctggag aaggagctca gggcgcgcgt tcttcttgaa gcgctccctt acattcgcga
     3001 attctccgga aaaactgtag tcatcaagta cggcggagcc gccctggatg accaggcaag
     3061 tcttcgcgct caattcgcag aagacattgt tcttctacat tatgtaggca tcaagcctgt
     3121 gatcattcac ggaggcgggc ctcgaatcaa ttctcttctg aagatgatgg gaattcaatc
     3181 tcagttttct ggtggagtgc gtgttacaga cgacgctacc atggaagcag tcgagatggt
     3241 tctgtccgcc agcatcaata aggaaatcgt ttcgttgatc cagagcaaag gaggccgcgc
     3301 cgttggcatt tccggacgcg acggcaacct ggctcacgct aaaaagacaa aacctgaact
     3361 ggggcgagtg ggtgaagtca cgccttctga cattgacccc gctgtcattc gcactcttct
     3421 tcatggcgga tacattccag tcatcgcacc tgttgcttca gatgaaaacc gcgatagcct
     3481 caacgtaaat gcagatacaa tggccggagc gatagcgtct gccctggggg cagaaaagtt
     3541 tgtactactt acggatactc cgggtgttct gagcaaaggg gaaacggtcc ccaggctcag
     3601 tatggattct gctcgcgcca tgattcagtc tggagaaatt tcaggcggga tgattccaaa
     3661 agttcaatgt tgtatgtctg cctttgagca gggagtgaag cagattcata tcgtagacgg
     3721 cagagttccg cacgccatca tacttgaaat tttcacccgc cagggcgtgg gcacgatgat
     3781 tcagtgaaat agaggaccca atgacaaaag ccgaaaaact cgtgaaagaa gtagtaagaa
     3841 ccgttccaga ttggccggaa aaaggcgtga tgtttcgcga tatcactccg attttccagc
     3901 atcctgaagt gctgcatgcg gtgtttgaag cgttcattga acgctaccgc gaaaagcagg
     3961 tggatgtgat tgctggaatc gacgcgcgtg gttttctcct gggtgtgacc atttcttacg
     4021 agctcaagat cccttttgtg cccgtgcgaa agagaggaaa gctgccatgg aagaccgtat
     4081 gggaagaata cactcttgag tacggaaccg ctgccattga aatgcatgaa gatgcatgca
     4141 agaagggcga tcgtgtggtg atctttgatg atctcattgc aactggcgga accatgcttg
     4201 ctgctgctag cattgtgaaa cgtcttggtg gcgaagtggt ggaagcggcg gctatcattg
     4261 atttgccaga cctgggtggc tcccagaaaa tatggaaaac gggcgttcca gtacacactc
     4321 tgtcaaagta cgaaggccac tgatcgtacg cgccgttttc gcgatggcgc tgcttctggc
     4381 tgcgcctgtt tctattttcg cgcgattacc tgcgtatgcg gatgtgcaaa agaaacttcc
     4441 tgacctctgg gccgccaggt ttccagtccc attgcaatcg tctgcaagtg atcccggaaa
     4501 aaggggaatg ctctattgcc tgatcgatgg acgtcctgcg tattatttta attttgcggc
     4561 tac
//
LOCUS       DAORFF010000301         4554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_10969_length_4554_cov_3.723050, whole genome shotgun sequence.
ACCESSION   DAORFF010000301 DAORFF010000000
VERSION     DAORFF010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10969_length_4554_cov_3.723050"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKK76_12200"
     CDS             <1..189
                     /locus_tag="PKK76_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69284.1"
                     /translation="YAVMGDPVNLAARLQELSKTLSVDIVIDGTTKSKIPASYAVRDS
                     GVKNVRGRHGEVEAWILS"
     gene            complement(196..645)
                     /locus_tag="PKK76_12205"
     CDS             complement(196..645)
                     /locus_tag="PKK76_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69285.1"
                     /translation="MRRILPCFALILGACTNYSAETQNVNPPILTSIAREGTGHIITM
                     AAQNTEIGFAGYRLFQGTTDTGVRTANSLSGIDCSRPLAVLPNQAISYVIEVKPGQTA
                     VSAGYSNRVCAVPLALTAGTLVGVRSLILRDLLSLDTGLSSNTLVVP"
     gene            complement(642..1433)
                     /locus_tag="PKK76_12210"
     CDS             complement(642..1433)
                     /locus_tag="PKK76_12210"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HNL69286.1"
                     /translation="MTRKIALFDMDYTIIPYDTLILFAGYVIRRKPWRIFYLALFVPA
                     AAAYVLGLTGRGGLKRAFLSVLWRLRKEEVHSLAESFSRDVLEGLLYKDLIEEAARIK
                     ADGGQLILNTASPSFYVNHIAKAAGFDEVVATEVVLEERMPLFPRIHSKNNRGIEKLL
                     RMPEICGESLIESIRTDYERLSKNPSLFDARPIANSTGYSDSSADLPLLRICEKKTLV
                     HPSKNLEELGKAQSWRILRPRRPYSGKIEKYVRLCLCLVGLLRLQ"
     gene            complement(1430..2377)
                     /locus_tag="PKK76_12215"
     CDS             complement(1430..2377)
                     /locus_tag="PKK76_12215"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNL69287.1"
                     /translation="MKRVLITGSRGFLGQHLCLSLLRDGYEVTGLDRSGKDRKPPSWW
                     TLYPDSKPDQIQQVESDLGSCDQIRGVLEQQSYDFILHLAGTAFVPEGWINTADVAVN
                     NTVVPIRLFQAARDANFKGRIVFVSSGEVYGAKHPMPLLEDFTLEPENPYAESKRSAE
                     QYAGYFVSRGLDIVIARPFNQIGPGQNPAFVIPSFLCRIRDAAARGETEIQVGDLDAE
                     RDFLDVRDAARAYSIVAEKGIKGQAYNICSGRPVTIRRVLDLALKVTGANLKPRIDPS
                     LLRDGKSIRYGSCEKLMNLGWSSSVELEESIRDIWRSMQ"
     gene            complement(2374..3666)
                     /locus_tag="PKK76_12220"
     CDS             complement(2374..3666)
                     /locus_tag="PKK76_12220"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNL69288.1"
                     /translation="MKETAFFWFRRDLRLKDNSGLFHALSSGLAVIPVFIFDTNILEN
                     LPRDDARVTFIHSVLTRLKTFLESKGSTILVMHGDPLTVWKELIAKHKPRAIFANHDY
                     ESYAIERDQKISEICKSSGVDFKTTKDQCIFEKSEVVKDSGEPYTVFTPYFRKWKVRL
                     ESFDMTAFPSEKRLERMARLPASKMPGLDQLGFVPSSIRIEDPSIPENIIASYAETRD
                     YPSLRGTTHLGVHLRFGTLSIRTLAKSAQKSQVFLSELAWRDFFMMILYHFPHSATRS
                     FKPQYDRIVWRNNENEFEAWKEGRTGYPLVDAGMRELVQTGFMHNRVRMVTASFLCKH
                     LLIDWRKGERFFAEKLLDYDLSANVGNWQWACGSGCDAAPYFRIFNPSAQEKKFDPDR
                     AYIKQWIGELSSSYPEPIVKHELARDRCLKVFKEALAK"
     gene            complement(3663..>4554)
                     /locus_tag="PKK76_12225"
     CDS             complement(3663..>4554)
                     /locus_tag="PKK76_12225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNL69289.1"
                     /translation="IVITGGTGLLGKATARTLESGGHEVRILSRNASARFRWDPVHAY
                     IDKSVFEQADAVVHLAGETISGRWTAEKKRQIIESRSRSTDLLAEAISSTGLKPVVIC
                     ASAIGYYGDRGKHVCMETDGPGSGFLCDTTRIWEERLADIPASRKVILRIGVVLSMLG
                     GALPQMVLPVKMFAGSPCGSGRQYISWIHIEDMVKMICCSIDRSMEGALREDLSGTYN
                     AVAPHPVINDDFMREIAATLSKPYFLPAVPSFLLKGMLGEMSVLLTDSIRVSADRIQD
                     AGFSFKFPDLKAALGSLLKK"
BASE COUNT         1079 a         1147 c         1149 g         1179 t
ORIGIN      
        1 tacgccgtga tgggagatcc tgtgaacctg gcggcacgtc tccaggagtt gagcaaaact
       61 ctgagcgtgg atattgtcat cgatggaact acaaaatcta agattccggc cagctatgct
      121 gtgagagatt ccggcgtcaa aaacgtcaga ggacgccacg gcgaagtaga agcatggatt
      181 ctgagctgag agcgctcaag gaaccacaag cgtattagag gaaagtccgg tatccagaga
      241 caataggtca cgcagaatca aagatcgcac tcctaccagt gtccccgcag tcagagccag
      301 tggcacggcg cagactctgt ttgaataacc ggcgctgact gctgtttgtc ctggtttcac
      361 ttcaattaca tagctgatgg cctggttcgg aagaactgcc agaggcctcg agcagtcgat
      421 ccctgaaagc gaattggcag ttctcactcc agtgtccgtg gttccctgga acaacctgta
      481 gcctgcaaat ccaatctcag tgttttgagc tgccatagtg atgatgtggc ccgttccttc
      541 tcgcgcaatg gaagtgagta tgggcggatt tacattctgc gtttccgccg aatagttggt
      601 acaggccccg aggatcaggg caaagcatgg gaggattcgc ctcattgcag tctgagcagt
      661 cctaccagac acaggcagag ccgcacgtac ttttcgattt taccggaata gggtcttctg
      721 ggtcgcagaa tccgccacga ttgcgctttg cccagttctt ccaggttttt cgaaggatgg
      781 acgagcgttt ttttttcgca aattcgaaga agaggaaggt ctgcgcttga gtcagaatag
      841 ccagtcgaat ttgcaatcgg ccgcgcatca aaaagagacg gattcttact cagtctctca
      901 tagtccgtgc gaatggattc gatgagagat tcaccgcaga tttccggcat gcgaaggagc
      961 ttttcaattc cgcggttgtt cttgctgtgg attctcggga aaagaggcat gcgctcttcg
     1021 agcaccactt ccgtggccac tacttcgtca aatcccgccg cctttgcaat gtgatttaca
     1081 taaaaggacg ggcttgctgt attcagaatc agttgtccgc cgtctgcttt gatgcgagct
     1141 gcttcttcta ttaagtcttt gtagagcaag ccttcgagca catctcggct gaatgattcc
     1201 gcaagtgaat gcacttcctc tttgcgcagt ctccatagaa cggatagaaa tgcacgtttc
     1261 agtccgcctc tacctgtcaa accgaggaca taggctgcgg ccgcaggcac gaagagcgcc
     1321 agatagaaga ttcgccaggg tttgcgccga attacatatc cggcaaagag gatcagagtg
     1381 tcatatggaa tgattgtata atccatatcg aatagagcga ttttcctggt cactgcatgg
     1441 atctccagat gtcgcggata ctttcttcaa gctctacgct cgacgaccat ccgaggttca
     1501 ttagcttttc gcatgagcca tagcggatgc tttttccgtc tcgaaggaga gaaggatcga
     1561 ttcgtggttt caggtttgcg cctgtgactt tcaatgcaag atcgaggact cgccgaatgg
     1621 taacaggtct tccggagcat atattgtacg cctggccttt gattcctttt tccgcaacaa
     1681 tcgagtaagc ccgggccgcg tcgcgcacat ccaggaagtc acgctctgcg tccagatcgc
     1741 ctacctgaat ttcagtttca cctctggctg cggcgtcccg gatgcgacag agaaaagagg
     1801 gaattacaaa agctggattc tggcctggtc caatttgatt gaatggtcgt gcgatgacta
     1861 tgtccagccc gcgcgaaaca aagtaccctg cgtattgctc cgcggatcgc ttactctctg
     1921 catatggatt ttctggctcg agagtaaaat cttccagcag aggcatagga tgcttcgcgc
     1981 catacacttc tcccgaactt acaaacacaa ttctgccttt gaagtttgcg tctcgcgccg
     2041 cctgaaacaa tcggatcgga acgacagtgt tattgactgc gacgtctgcg gtattgatcc
     2101 agccttctgg cacaaaagca gtgccggcca gatgaagaat aaaatcgtac gattgctgtt
     2161 cgagtactcc tcggatttga tcgcagctcc ccagatctga ctctacctgt tgtatttgat
     2221 ctggcttcga atccggatac agtgtccacc aggaaggcgg cttgcgatct ttgccagaac
     2281 gatcgaggcc agtcacctca tagccatcgc gaagaaggga aaggcacagg tgctggccca
     2341 ggaatcctct gcttcctgtg atgagtactc gtttcatttt gccagagcct ctttaaacac
     2401 tttcagacag cgatctcttg ccagttcatg tttcacaatc ggttccggat aggagctcga
     2461 aagctctcca atccattgtt ttatataggc ccggtcgggg tcgaactttt tctcctgcgc
     2521 tgacggattg aaaatcctaa agtaaggggc agcatcgcat cctgaaccgc atgcccattg
     2581 ccagtttcca acattcgcag aaagatcata atcgagcagt ttttctgcga agaaccgttc
     2641 cccttttctc cagtcgatga gtaggtgttt gcaaagaaag cttgcagtga ccattcgcac
     2701 acgattgtgc atgaaacccg tttgtacgag ctcgcgcatt cctgcgtcta ccagagggta
     2761 accggttctc ccttctttcc atgcttcgaa ttcattctca ttgtttcgcc acacaatgcg
     2821 atcatactgc ggtttgaagg atcgcgtcgc ggagtgcgga aaatgataga ggatcatcat
     2881 gaagaaatcg cgccaggcga gctctgacaa aaacacctgt gacttctgtg cgctctttgc
     2941 aagtgttcga atgctcagag ttccaaacct gagatgcact cccagatgtg ttgtgccgcg
     3001 aagtgaaggg tagtcgcgcg tttcggcata agatgcgatg atgttttccg gaatcgaagg
     3061 atcttcgatg cgaatggagg aaggaacaaa tccgagctga tcgagacccg gcatttttga
     3121 tgcaggcagc ctggccattc tttcgagcct tttctccgat ggaaaggcag tcatgtcaaa
     3181 cgattcgagc cgaaccttcc atttgcgaaa gtacggcgta aacacagtgt agggttcacc
     3241 cgagtctttc actacttccg atttttcgaa tatgcattga tctttcgttg ttttgaaatc
     3301 tacaccggat gatttgcata tctctgaaat cttttgatct ctctctattg cataagattc
     3361 gtagtcgtga tttgcgaaga tagcgcgcgg tttatgtttt gcgatgagtt ctttccatac
     3421 agtgagtgga tctccgtgca tcaccagaat cgtgctgcct ttgctttcca gaaaagtctt
     3481 aagtctcgtt agaacagaat ggatgaaggt cacgcgcgcg tcatcgcgtg gaagattttc
     3541 gaggatgttt gtgtcaaaga taaatacagg aatgacggcc agaccactcg atagtgcatg
     3601 aaagagcccc gagttgtcct tcagtcgcag gtcgcggcga aaccagaaga aagcagtctc
     3661 cttcatttct tgagaagtga tccaagcgcg gctttgagat ctggaaattt gaaactaaat
     3721 cctgcgtctt gtattctgtc tgccgatacg cgaatgctgt ccgttaagag gacagacatt
     3781 tctccgagca ttcccttcag taagaaagag ggcacagcag gcagaaaata aggcttcgaa
     3841 agagtggccg cgatttcgcg catgaaatcg tcattgatga ccgggtgagg agccaccgca
     3901 ttgtatgtgc cggatagatc ttcgcgcaaa gctccttcca tggagcgatc tatgctgcaa
     3961 cagatcattt tgaccatgtc ttctatatgg atccaggaaa tgtattgtct gccggatcca
     4021 cagggcgatc cagcgaacat cttcacgggc agtaccattt gcggtaaggc tccgcctaac
     4081 attgacaaga cgactccgat tcgaagaatc actttgcgtg aagcggggat gtccgcgagc
     4141 ctttcttccc agattcgagt tgtatcgcac agaaacccac tccccgggcc gtccgtttcc
     4201 atgcaaacat gctttcctct gtctccatag tagccaatgg ccgaagcgca aatcacaaca
     4261 ggttttaagc cggttgagga aatggcttcc gccaggagat cggtgcttct gctgcggctc
     4321 tctatgatct gtcttttctt ttccgccgtc caccttccgg agatcgtttc tcctgcaaga
     4381 tgaaccacag catctgcttg ttcaaaaacc gatttgtcta tgtaagcatg cactggatcc
     4441 catctgaatc tggcgcttgc gtttcgagat agaatgcgaa cctcgtgccc gccgctttcc
     4501 agcgttcggg ccgttgcctt acccaggagc cctgttccgc cggtgatgac gatg
//
LOCUS       DAORFF010000302         4545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11004_length_4545_cov_3.353452, whole genome shotgun sequence.
ACCESSION   DAORFF010000302 DAORFF010000000
VERSION     DAORFF010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11004_length_4545_cov_3.353452"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1996)
                     /locus_tag="PKK76_12230"
     CDS             complement(<1..1996)
                     /locus_tag="PKK76_12230"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNL69290.1"
                     /translation="MPGYGRLLIWAVIGLASVSAHRIFFAARFHGEEQILDVIVSFAA
                     GLRFDLAVLPVVSGPYILIESILFQFAGRAIDIARRALMSLQIIWLSILNISLYASTF
                     NYSVNEKHLGWEFFAYINDLGSLWRDVFEKDVASGLFYGLVPLVFLAGAIAVFRKQGG
                     FHSDEPRRFETRFKKRKLFATLGIFVLLGIFLRGGLQQSPLRTADAMTTDSPYLNNLK
                     LNGIFTVLNDSRDSLEFKSFYSREENIKTMTQFLSAGKISKDYPLLRRMPPRTLASAR
                     PVSHPRVILVVLESFSSKFLAVHGYDPSIAPQFNELIHRGVYFRRFFATGGRSANGLF
                     AMFAGIPDRAGRTILRSSQIQNRFGGLAGILGRKGYRTFFVHGGDLKFDNLNTALPHL
                     GFSESYGRDEIAATGFQAEPNVLGYDDRDAFRFFLGQMDKKDQSFGVIFTQNTHFPYI
                     APDYKDYSKDRVANLFLSSYHYVDSLIGEFVRELEKRPYAKDTIVLFVADHTHHAGLN
                     YIEDRSIPFLVYAPSLFSPAQIDFDASQLDILPGIVSLTGGNALYASMGNDPFLRAGL
                     VRGEPEVLPEPFAFFAGGSNTNVIGWIQKDRIAIRWLDRDTPLFLTSTPPITGENFAL
                     KEKSRLDDYTAHMRHYHQYARTLEKENRIWPEPDELAKLEK"
     gene            2095..3090
                     /locus_tag="PKK76_12235"
     CDS             2095..3090
                     /locus_tag="PKK76_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HNL69291.1"
                     /translation="MKADVYGVGNSLVDTLAFVDQDFVREHDLAPGSMTLVEAEKQAA
                     ILLGLSRHQLELRSGGSAANTMIAVARSGGTSFYTGKVSRDTNGEFYRQDLLAAGIHF
                     DVHPAAETDGPTGTCVVLTTPDAERTMLTHLGVSIQLSANDINEDRIKASKMIYIEGY
                     LWAGESTRAACIRAAEMGRKHQVPVAFTFSDSFLVAPFRSDFFGFVKDYCSVVFCNAP
                     EVRSFSEMDKLEDAALYAGQIADTVFVTNSEHGALVVEHGKITEVPGFRVKAIDTNGA
                     GDAFAGGTLFGLCRGYSSAHSARWGNFLASEIVQIPGARLTRDYSSQVRTIIGSP"
     gene            3077..4219
                     /locus_tag="PKK76_12240"
     CDS             3077..4219
                     /locus_tag="PKK76_12240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNL69292.1"
                     /translation="MARLDPSRISVLHLNAARTWRGGEQQTLYLAEGLAKAGIRQAII
                     GRPGSELSSRASDKGIEFIPVAMGSEADFLSAFRIATIAREGSYRILHAHTAHAHSLA
                     ILARLFFRDLRVVVTRRVDFHIRGLSGLKYRTSLVDRFCAISANVRDIMLHDGVPEDR
                     IRLAYSGIDLKRFKKLPSSADLRKEFRIGRELVVGCVGALVDHKDQRTLIRAFGNLPE
                     AGEKIGRGIMPRAVLILVGDGNLKEEYEQIATDLFGVGLKARGHRIVFAGFRADIPAF
                     LSLFDIFAMSSKEEGLGTSVLDAMAAGLPLAVTDGGGLREMADPGKGALVSPARDPER
                     LAENLTALLTQKALRDRFSAHNRRRVKDFSVEEMVKANLAVYRELV"
     gene            4216..>4545
                     /locus_tag="PKK76_12245"
     CDS             4216..>4545
                     /locus_tag="PKK76_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69293.1"
                     /translation="MKFTDIFRRVNHRVREARDRMTEKSREAGDRMRSVIDTFSDEWM
                     ESAFHRYRQMLAPSPDSLPSDFRLRESSLPRRIYERLFPVDHVFRMSWKYGAPFLEDF
                     MPISPDAF"
BASE COUNT         1135 a         1069 c         1234 g         1107 t
ORIGIN      
        1 ctttttccag tttcgcaagt tcgtctggtt ccggccagat ccggttctct ttttccaggg
       61 tgcgcgcata ctgatgatag tgacgcatgt gcgctgtgta atcgtccagc ctcgattttt
      121 ctttaagggc aaaattttcg ccggtgattg gaggagtgga agtcaaaaac agcggagtat
      181 cgcgatccag ccaacgaatg gcgattctat ctttttgaat ccagcctatt acattggtgt
      241 tggacccccc ggcaaaaaac gcgaatggct ccggcagaac ttctggttct ccgcgaacca
      301 ggccggcgcg aaggaatgga tcgttgccca tggaggcgta gagagcattt cctccggtca
      361 gcgaaacgat ccctggaaga atatccagct ggcttgcgtc aaagtcgatt tgtgcagggg
      421 agaaaagaga aggagcatac accagaaaag gaattgagcg atcctctata taattcaatc
      481 ccgcgtgatg tgtgtgatct gcgacgaaga gaacgattgt gtctttcgca tacggacgct
      541 tctccagttc tctcacaaat tctccaatta gagaatccac gtagtgatag ctggacagga
      601 agagattggc cactctatct tttgaataat ctttgtagtc cggcgcaatg tatgggaagt
      661 gagtattctg ggtgaagatg acaccaaagg actgatcctt cttatccatt tggccgagga
      721 agaatcggaa cgcatcgcga tcgtcgtatc caagtacatt cggttctgcc tgaaatccgg
      781 tggctgcgat ttcgtctctg ccgtaggatt ccgaaaagcc aagatgtgga agggcagtgt
      841 tcagattatc aaacttcagg tctccgccat gaacaaagaa tgtgcgataa ccttttctac
      901 cgaggattcc cgccagtccg ccgaagcggt tttgaatctg cgacgatctt agaatcgtcc
      961 ggcctgcgcg atcggggatc ccggcaaaca tagcgaagag gccgttggcg ctacggccgc
     1021 ctgtcgcaaa gaaacgacgg aaatacacac cgcggtggat caattcattg aattgcggag
     1081 caatggaagg atcatatcca tggaccgcca ggaatttgga gctaaacgat tcgagcacca
     1141 caaggatgac tctcgggtgc gatactggcc tcgcagaagc aagggtgcga ggcggcattc
     1201 ttctgaggag tggatagtcc tttgatatct ttccagcaga aagaaactga gtcatagttt
     1261 tgatattctc ttcgcggcta tagaacgatt taaattcgag agaatcgcgc gaatcgttta
     1321 acactgtgaa gatgccgttc agcttgagat tgttgaggta gggcgaatcg gtagtcattg
     1381 catctgcggt gcgaagaggg ctctgttgaa gaccgccgcg aaggaagatc cctagcaaaa
     1441 caaagatccc cagagttgcg aagagctttc ttttcttgaa tctggtttca aatcggcgcg
     1501 gttcatccga gtgaaagcca ccctgcttgc gaaatactgc gatggccccc gccagaaaaa
     1561 cgagagggac aagaccgtag aagagtccag acgctacatc tttttcgaat acatcacgcc
     1621 acaaagatcc gaggtcgttg atgtaggcga agaattccca gcccagatgt ttttcattca
     1681 cactgtaatt gaaagtggat gcgtacagac tgatattcag aatagacagc cagatgatct
     1741 gcaaggacat gagcgcgcgt cgcgctatgt caatggctct gcctgcaaat tggaacagaa
     1801 tgctttcgat caggatgtag ggacctgaca ctacgggcag cactgccaga tcaaacctga
     1861 gtccggcggc gaaagaaaca atgacgtcca gaatttgttc ttcgccgtga aatctcgcgg
     1921 cgaaaaaaat cctgtgggct gacacgctgg caagaccgat gactgcccag atcaagagtc
     1981 tgccgtaacc cggcatgcag caccatcgcc tgaagcgact tccagcgcaa tttcaatttg
     2041 catgcggtgc cattttactt gccctccgtg tcctggcctc aaaattcaga aaatatgaag
     2101 gccgatgttt atggtgtggg caactcgctg gtggataccc tagcgtttgt agatcaagat
     2161 tttgttcgcg aacacgatct ggcacccgga tccatgactc tggtagaagc ggaaaaacaa
     2221 gccgcgattt tgctcgggct gtccaggcat caactggaac tgaggtcggg tggatctgcc
     2281 gcaaacacta tgatagcagt ggcgcgaagc ggtggaactt ctttctacac tggtaaggta
     2341 agccgtgata cgaacgggga attttacaga caggatttgc tggcagccgg gattcacttt
     2401 gatgtgcatc cggcggctga gacagatggg cccactggaa cctgcgttgt tcttaccaca
     2461 ccggatgcag aaagaaccat gctcactcat ctaggcgtgt ccattcaact gtctgcaaat
     2521 gacatcaacg aggacagaat caaagcaagt aagatgatct acatcgaagg ctatttgtgg
     2581 gcaggggaat ccacgagagc agcctgcatt cgcgcggcgg aaatgggaag aaagcatcaa
     2641 gttcctgttg ccttcacttt ttctgacagc tttcttgtgg ctccttttcg atctgatttc
     2701 tttggatttg taaaagacta ttgttctgtt gtattctgca acgcccccga agttcgaagt
     2761 ttcagcgaaa tggacaagct ggaagacgcg gcgttgtacg caggacagat cgcagataca
     2821 gtatttgtaa ccaatagcga acatggtgct ctggtcgtgg aacacggaaa aatcacagaa
     2881 gtgcctggct ttagagtcaa agcgattgat acaaacggag caggggacgc ttttgctggc
     2941 ggaacgttat tcggcctttg caggggatat tccagcgctc attctgcgcg ctggggaaat
     3001 tttctggcaa gtgaaatcgt tcagattcct ggagcgagac tcacgcgaga ctactcaagc
     3061 caggtgagaa ctatcattgg ctcgccttga tccgtcacgg atttccgtcc tacatttaaa
     3121 cgcggctcgc acctggcgcg gcggtgaaca acaaacatta tacctggcag agggtctggc
     3181 taaagcaggc atccgtcagg cgatcattgg cagacctggc agtgaacttt cttcgcgcgc
     3241 gagtgataaa ggaattgaat tcattccagt ggccatggga agtgaagcgg acttcctgtc
     3301 cgcatttcgc atcgccacca ttgcgcgcga agggtcgtat cgcatcctgc atgcgcatac
     3361 agctcacgcg cacagtctgg ccattctcgc gaggctcttc tttcgcgacc tcagggtcgt
     3421 tgtgaccagg cgggtagatt ttcacattcg cgggctatcc ggtttgaagt accgcacttc
     3481 tcttgtggat cggttttgcg caatttcggc taacgttcgt gatatcatgc ttcatgatgg
     3541 tgtgccggaa gatcgaatcc ggctggctta ctcgggaatc gatttgaagc gcttcaagaa
     3601 actcccgtct tctgctgatc tgaggaagga atttcgaatc ggtcgcgaac ttgtagtcgg
     3661 atgtgtgggc gcactcgtgg atcacaaaga ccagagaact ttgatcagag cgtttggaaa
     3721 tctgcccgag gccggagaga agattggtcg tgggattatg ccgcgtgctg tattgatttt
     3781 agtaggggat ggaaatctga aagaagaata cgagcaaatt gccacggatc tgttcggtgt
     3841 gggcctcaaa gcacgtggtc acagaattgt tttcgccgga tttcgcgccg acattcctgc
     3901 tttcctttct ctctttgata tctttgccat gagctcaaag gaagaagggc tgggtacttc
     3961 tgtactcgat gcaatggctg cgggacttcc tcttgccgta acagatggtg gaggactcag
     4021 agaaatggca gatccaggga agggcgcact cgtttctcct gctcgtgatc cagaaaggct
     4081 ggcggagaat ctgacagctc ttcttaccca gaaagctctt cgcgacagat tcagcgccca
     4141 caacagaaga agagtgaagg atttcagcgt agaagagatg gtgaaggcaa acctggccgt
     4201 ttacagggaa cttgtttgaa attcacagat attttcagaa gagtcaatca tcgtgtgcgc
     4261 gaagcccgcg atcgcatgac ggaaaagagc agagaagccg gcgataggat gcggtctgtc
     4321 attgatacat tcagcgatga atggatggag tctgcgtttc atagatacag acagatgctc
     4381 gcgccgtctc cggacagtct tccatctgat tttcgcctgc gcgaatccag tctccccagg
     4441 cgcatttacg aaagattatt tcctgtggat catgtgttcc gcatgtcctg gaagtatgga
     4501 gctccgtttc tagaagattt catgcccatc agtccggatg cgttt
//
LOCUS       DAORFF010000303         4529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11062_length_4529_cov_4.470496, whole genome shotgun sequence.
ACCESSION   DAORFF010000303 DAORFF010000000
VERSION     DAORFF010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11062_length_4529_cov_4.470496"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1662)
                     /locus_tag="PKK76_12250"
     CDS             complement(61..1662)
                     /locus_tag="PKK76_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/carbon-nitrogen hydrolase family
                     protein"
                     /protein_id="HNL69294.1"
                     /translation="MAEELRKPVAEKPLEKPAEKKRVPRHKLKLRNLTLADFEDVREI
                     MNLVYADFGGTWTREEFGEQVNRFPEGQLCIENKGKVVACALSVIVDYSKYGDNHNYN
                     QITGNGKLTTHDPKGGTLYAVDVFVHPQFQGLRLGRRLYDARKELCRKLNLRRIIAGG
                     WIPGYKQQIDKMTPQQYIELVKQKELYDHVLSFQLANDFHVRKVMLNYFPENKGGYNS
                     VATLLEWINIDYVEPGQPQPLFPKKSVVRVGVIQWQMRRVDSVPDLIQNIEFFVDAVS
                     GYKADFVVFPEFFNAALMAPFNKMMPGDAMRQLSTYTEQIRNEMQQMAVSYNVNIISG
                     SMPEYREHQLYNVSYLLRRDGTFDEQYKLHITPDESNYWGLKGGNKIQVFETDIGKVG
                     ILICYDVEFPELARVLAEQGMEILFVPFSTDTKNAYLRVRHCAHARAIENECYVVISG
                     NVGNLPNVENMDIQYAQSGIFTPSDFLFSQDAVAAEAVPNTESTLIVDLNLDLLKQVR
                     TTGSVRNLENRRLDLYKIHWHEFQG"
     gene            complement(1741..2073)
                     /locus_tag="PKK76_12255"
     CDS             complement(1741..2073)
                     /locus_tag="PKK76_12255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL69295.1"
                     /translation="MEISHRTQKETDIIQLRGELDMFNSPELGSFIRGRIDSGSKTVL
                     VELEDVTYLDSSGLGVLISAATRMMKASGQFFLVRPSHQVRRLLELTRLTAFFRILNS
                     RDEFLRAT"
     gene            complement(2102..3361)
                     /locus_tag="PKK76_12260"
     CDS             complement(2102..3361)
                     /locus_tag="PKK76_12260"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL69296.1"
                     /translation="MTATLESERSERRTNLRPVFSAYELGKICIHARTLAQVTGSDFV
                     SETTPVVELYDLLSANPDLDFLPLTGPDGDVSGYIRRQMFFAKLSQTRFSRELLLRQD
                     VRAHSIMERRVICLDARTYLSEASEALMARDEEIRFDPFVVTYEGKFFGTATVQRVLE
                     GINHFLKLDLEACARMQDQILQPLERGFEITSDNHRIVMPLIAPGGDYAELIEINDRY
                     SLGLLFDVCGKGLKASSMVNTFASVIKTLIYKMDAEGSDLPRILLALHNSNQLLTQLT
                     CQEMYATGVAIILDKEKNVISVLDYGHGYVWLKRGSSVHRLSAHIKHAMAVPFLGIHD
                     DLQMPAGHFLVQNNDVIFACSDGLIEQKNDQREDYGVQRLRNLLRRVSPESPRQINET
                     ILEDFNMHIAGSRRSDDLCMLCIRVVV"
     gene            complement(3419..>4529)
                     /locus_tag="PKK76_12265"
     CDS             complement(3419..>4529)
                     /locus_tag="PKK76_12265"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNL69297.1"
                     /translation="PSWVTEWNAKRIPDSYFNSKWELSVSRESLSRLWKSPFDHANSK
                     AKLGTRFPHYLTAGLSEPDKSFYTAPTISPFGNEIVFDFAKELITREKMGQDSVPDLL
                     AISLSSNDHAGHQYGPDSPEVLDVAVRTDKLLSNFLIFLDQNIGLDNAIIVLSGDHGV
                     LSNSRLLAEFGFPAGNYDERAICRSVLSDLRREFGPGDWIYGGDPSGPVRYETRSGCV
                     EDSIFLNRELIARRNIPLELIQSSAARSVSRWPGIYSAYSASDILKNRLPQNDIAARL
                     MRSYYPPIGPDVIYIPEAFWSSADYTLGATHGTPYSYDTHVPILMSGAGIAPGDHDER
                     VSTLDIAPTLSGLLHILPPSGSEGRILPVSLKTGR"
BASE COUNT         1059 a         1118 c         1122 g         1230 t
ORIGIN      
        1 gccgccgggc aggcggcgat cacgaaaaat cagtttgcag taaaaaattt tgtccgattt
       61 ctatccctgg aactcatgcc agtggatttt gtatagatcc agtctcctgt tttccagatt
      121 tcgcacggag cccgtagtcc tcacctgttt gagtagatcg agatttagat ccacgatgag
      181 agtggattca gtatttggca ccgcctccgc cgcaaccgcg tcctgcgaaa aaaggaaatc
      241 ggacggagta aagattccgg attgcgcata ctggatgtcc atgttttcca cgtttggcaa
      301 gttgcccacg ttgcctgaga tcacgacata gcactcattt tcgatggctc ttgcatgcgc
      361 gcaatgtctg actctcaggt atgcgttctt cgtgtccgtt gaaaacggaa cgaacaggat
      421 ttccattcct tgttctgcga gaactcgtgc cagctccgga aattcaacat cgtagcatat
      481 gaggattcca acttttccga tgtcagtttc aaacacctga atcttgtttc ctcccttcaa
      541 tccccagtag ttggattcat ccggcgtgat gtggagcttg tattgttcgt cgaaagttcc
      601 gtctcgacgc agcaaatagc tgacgttgta tagctggtgt tcgcgatact ccggcatgct
      661 tccgctgatg atattgacgt tatacgacac tgccatctgt tgcatttcat ttcggatctg
      721 ttccgtgtat gtggatagct gccgcatagc atcgccgggc atcatcttat tgaatggcgc
      781 catcagtgcc gcgttgaaaa attcagggaa gacaacaaaa tcagctttgt aaccgctcac
      841 cgcatctacg aagaactcaa tgttctggat gagatccggc acggaatcta ctcttctcat
      901 ttgccactgg atcactccca ctcgcactac agactttttt gggaaaagag gctgtggttg
      961 ccctggttcc acgtagtcta tgttgatcca ttccaggaga gtggccacag agttgtagcc
     1021 gcctttgttt tctggaaaat agttcagcat cacctttcgc acgtgaaaat cgtttgcgag
     1081 ctggaagctg agaacgtgat cgtagagttc cttctgcttg actaattcga tgtactgttg
     1141 cggagtcatc ttgtcgattt gctgtttata tcctggaatc cagccgcctg caatgatcct
     1201 gcgaaggtta agttttctgc agagttcttt tcttgcgtcg tacagtctgc gacccagccg
     1261 caatccctgg aactgcggat gcacaaatac atccaccgca tagagagtcc cgcctttcgg
     1321 atcgtgcgtg gtgagcttcc cgtttcctgt gatttgattg tagttgtgat tgtctccgta
     1381 tttcgaatag tccacgatca cgcttagggc gcaagcgacg accttccctt tattttcaat
     1441 gcagagttgc ccttccggga atctgttgac ttgctctccg aattcttctc tggtccatgt
     1501 gccaccaaaa tcggcataga ccagattcat gatttcgcgt acgtcttcga agtccgcaag
     1561 agtgaggttc cgcagtttta gtttatgacg cggcacgcgt ttcttctctg ccggtttttc
     1621 cagcggcttt tccgcgactg gtttcctcag ttcttcggcc atgatgtctt ttgacgccga
     1681 gcgagcccag agcaagcaaa taaagcccgg aatgcgacgg cctgcacaat gacttttctc
     1741 tcatgtcgct cgcaggaatt catccctcga attcaggatt cggaaaaaag cagtcagacg
     1801 agtcaattcc agcaatcttc gcacctgatg ggaagggcgc acgagaaaga actggcccga
     1861 cgctttcatc attctggtag ctgcgctgat aagaactccg aggccgcttg aatccagata
     1921 ggtcacatct tcaagttcca caaggacggt cttgcttcct gaatcgattc ttcctcgaat
     1981 gaaactcccg agctccggcg aattaaacat atcgagttct ccgcgcagct ggatgatgtc
     2041 tgtttctttt tgggttctgt gactgatttc catatttttc tccaataata aattttataa
     2101 actaaacaac tacacgaatg caaagcatgc atagatcatc ggatcttctg gatccagcga
     2161 tgtgcatatt gaagtcttca agaatcgttt cattgatctg ccgcggcgat tcaggtgaaa
     2221 ctcgcctgag cagattgcgt aggcgctgaa ctccgtaatc ttcgcgctga tcgttctttt
     2281 gttcgatgag tccgtccgag caggcgaaga tcacatcgtt gttctgaacc agaaaatggc
     2341 ccgcaggcat ctggaggtca tcgtggattc caaggaacgg tacggccatg gcatgcttga
     2401 tgtgggcact gaggcgatga acgcttgaac cgcgtttcaa ccatacatat ccatgaccgt
     2461 aatccagaac gctgatgacg tttttctctt tatccagtat gatggcaaca cccgttgcat
     2521 acatctcctg acaggtgagt tgcgtgagca gctggttaga gttatgaagg gcaagtagga
     2581 tgcgaggaag atcgctgcct tctgcgtcca tcttgtagat cagagtcttg atcacagatg
     2641 caaacgtgtt taccatggaa gaagctttga gtccttttcc gcacacatcg aatagaagcc
     2701 ccagtgaata acgatcgttg atttctatga gctccgcata gtcgccgcct ggtgcaatga
     2761 ggggcatgac gattctatga ttgtcagatg tgatttcaaa tccacgttcc aggggttgga
     2821 ggatttgatc ttgcatcctc gcgcatgctt cgagatcaag cttgaggaaa tgattgatac
     2881 cttctagaac tctctgcacg gttgccgtgc cgaaaaattt tccttcgtat gtgacaacga
     2941 acggatcaaa tcggatttct tcatcgcgtg ccatgagcgc ttctgaggct tctgaaaggt
     3001 aggtccgtgc atccagacag attacgcggc gctccatgat gctatgcgcg cgcacatcct
     3061 gcctgagcaa tagttcgcgc gaaaacctgg tctggctgag tttggcaaaa aacatttgtc
     3121 tgcgaatata gccggagaca tctccgtccg gtcctgttaa aggaagaaag tctagatccg
     3181 ggttcgcgct cagtagatca tatagttcaa cgactggcgt tgtttctgag acaaaatcgc
     3241 tgccagtcac ttgcgccagc gtccttgcat gaatgcagat ctttcccagt tcgtatgcgg
     3301 agaaaacggg acgaagattg gtgcgacgtt ccgatctttc tgattccaga gttgctgtca
     3361 taggaacagt atgatcctga cgcgttactg ttccgtgtca taggtgtgaa tgtcaggttc
     3421 atctgccagt cttgagactg actggcagaa tgcggccttc tgaaccggaa ggaggcagga
     3481 tgtgcagcaa tcccgagaga gtcggcgcaa tgtcgagtgt ggagactctt tcatcatgat
     3541 cgcccggggc aattccagcg ccgctcatga gaattggcac atgcgtgtcg taagagtaag
     3601 gagtgccgtg tgtcgcgccc agagtgtagt ctgcggaact ccaaaatgct tccggaatat
     3661 aaatcacatc cggtcctatg ggaggataat agcttcgcat caggcgtgca gcgatatcgt
     3721 tttgcggtag tctatttttc agaatatctg aggccgagta tgcggagtag attccaggcc
     3781 agcgcgatac gcttcgcgcc gcagaagatt gaatgagttc caggggaata tttcttcgcg
     3841 caatcaactc tcggttcaga aaaatcgaat cctctacgca accgctcctg gtttcgtatc
     3901 gcaccggacc tgaaggatcc ccgccataaa tccagtctcc cgggccaaat tcgcggcgaa
     3961 gatcactgag caccgatctg caaatcgctc gctcatcgta gttgcccgcc gggaaaccaa
     4021 actccgccag aagcctggaa ttgctgagca ctccgtgatc tcctgacagt acaatgattg
     4081 cattgtccaa tcctatgttt tgatccagaa aaatcagaaa gttgctgagt agtttgtctg
     4141 tgcgaacagc cacatcgagc acctcgggac tgtccggacc atactgatgc cctgcgtgat
     4201 cgttgcttga gaggctgatg gcaaggagat ccggtacgga gtcctgtccc attttttccc
     4261 gcgtgatgag ttctttcgcg aaatcgaaaa caatttcatt tccaaacggg gaaattgttg
     4321 gagcagtgta aaaacttttg tccggctctg atagaccggc tgtcagatag tgaggaaatc
     4381 gcgtacccag ctttgctttg ctgtttgcat gatcaaatgg acttttccag agtcgagaga
     4441 gactttcccg ggaaacagat agctcccact ttgaattgaa atacgaatct ggaattcttt
     4501 ttgcgttcca ctctgtgacc cacgaaggg
//
LOCUS       DAORFF010000304         4529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11063_length_4529_cov_3.381538, whole genome shotgun sequence.
ACCESSION   DAORFF010000304 DAORFF010000000
VERSION     DAORFF010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11063_length_4529_cov_3.381538"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PKK76_12270"
     CDS             <1..1128
                     /locus_tag="PKK76_12270"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNL69298.1"
                     /translation="FKYYNFFASSAVQAAGWFGLHADFPVLSLILPVGISFYTFQSLS
                     YTIDVYRGVEPAEKSLPRLLFFVSFFPQLVAGPIVRARHFLLRLREDMRHLTPSMRQF
                     QTGIFLIMKGLTKKLAADWIAVQMVDRVYQNPGMFTSAEILAAVYGYGLQIYGDFSGY
                     TDIALGSARLLGYRLTVNFDRPYMSYSITDFWRRWHISLGSWFRDYLYIPLGGGRSHA
                     GLNLFITMTIAGLWHGAGWNFVIWGTFYGAMLAFERATGLSKTSTANGWKMLRILITL
                     HLVLFGWILFRVDSMETFRGVMGGLSRLEFRSPNLNWIQILVLCAGYGLHLVPVRWIL
                     RLRARYALLPVYAAATLCAIFLLIAGHLAVPQAVPFIYFQF"
     gene            1217..1792
                     /locus_tag="PKK76_12275"
     CDS             1217..1792
                     /locus_tag="PKK76_12275"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNL69299.1"
                     /translation="MSELRFRTLSQAEEHLERIEKAPAYKATGLWSEAQIFDHLANSF
                     ENSVSGYPGLLPAVLRKTIGRMIFRSQQKNGFMKPGSPNPNAPKVRAEGDAKQALKRL
                     RKAIVQFKAHDGEMAIHPAFDRLSKDEFSHLHAMHMANHLEHIHLEETKGRDARTARL
                     TGKTQSGKSGSRKSTGKRSASKKTGRSAKRK"
     gene            1839..2891
                     /locus_tag="PKK76_12280"
     CDS             1839..2891
                     /locus_tag="PKK76_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNL69300.1"
                     /translation="MLELLGAAASAIGSIASQPWAIALAGIASAVGIFLVKHFLTRAG
                     DDMYHWLRRKIFGRRKVESETQRELASRETRTARERFSPFEVETPVDRYSVTEYVARG
                     DIASIYGGTNRSDRQVILKVAMSTGDNDLLQNEANTLRALHQGDTVYNKHLPELLDQF
                     RASSGEIGLVLARIRASDLRTVRESYPQGIPQQHILWILRRTLAAVGHAHSRGIIHGN
                     IDPSHILISAPDHNVFVIDWAYAVHKPAETGQGFKAINDHFSPPEVAEKKQPLPASDL
                     YSIGKCMIYLLGGNVQLDTMPDNVDERIQRFIKFFTRTSALQRAQDAWQMYAKADDLR
                     EEVFGPHKFLEFKIQE"
     gene            2895..4355
                     /locus_tag="PKK76_12285"
     CDS             2895..4355
                     /locus_tag="PKK76_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69301.1"
                     /translation="MGGGYYSQDIAREARSSGGGVFSYQGYGVNAADASARREVHPLL
                     NVHGKNRECMNETAIVVALDVTRSRGNDTKVVYEKLPLFLGQIELKGYVKGAAISFAA
                     IGDANSDQAPLQVGQFEADNRLDEVLSKFWIEEGGGGTGQESYELAAYYYAKHSILDC
                     NRKGKKGFFFFVGDEGFYPSVSPDHVEKILGRSISGPVPSLQAFQALQEKYHVFFVQP
                     EKTWEERKADVDAEIQARVEAAGGQYKNVGVRASLIWNNRNDLDLHVICPSGEEIFYG
                     HKQSVCGGWLDVDMNVQGETTKPVENVRWSRGTAPKGRYRIFVQNYRIHEGSGPTDYR
                     VEIEINGEIKQFKGKMSAHGETSVESNQIIYEFDYDPSKRKVQDDPGEKATYAAYDDS
                     VIRKQWASVLPSSHILPIADPARILDVMLGAMAITAGTADISSFARDLKGRDHTDAQV
                     EEVRNALSGISIPSAGGSQFPGAGEGENRQSGSRRI"
BASE COUNT         1120 a         1085 c         1238 g         1086 t
ORIGIN      
        1 tttaagtact ataacttttt tgcgtcaagc gccgtgcagg ctgcaggatg gtttggactg
       61 cacgcggatt tccccgttct gagcctgatt ctccctgtgg gaatttcctt ttatacattt
      121 cagagtcttt cttatacgat cgacgtgtat cgcggtgtgg agccggcgga gaagagcctg
      181 cccagactat tgttctttgt gagtttcttc cctcaacttg tggccgggcc aatcgtgcgc
      241 gcgaggcatt tccttctgcg tctgagagaa gacatgcgcc accttacacc gagtatgcga
      301 cagtttcaga caggcatctt tcttattatg aaagggctta cgaagaaact cgcggctgac
      361 tggatcgctg tgcaaatggt ggaccgcgtt taccagaatc ctggcatgtt tacatctgcg
      421 gaaatactgg cggcagttta tggatatggc cttcaaatat acggcgattt ttctggttat
      481 actgacattg cgctcggaag tgccaggcta cttggatatc gcctgactgt aaattttgac
      541 cgtccttaca tgtcttattc catcactgac ttctggagaa gatggcatat ttctctgggg
      601 agctggttta gggattatct ctacattccc ctgggaggtg gtcgctctca cgccgggttg
      661 aatttgttca tcaccatgac gattgcgggg ctgtggcatg gcgccggctg gaacttcgta
      721 atatggggaa ctttctatgg tgcgatgctt gcgtttgagc gtgcgacggg tctgtcaaaa
      781 acgtccaccg caaatggttg gaaaatgctg cgcatcttga ttactctgca cctggtcttg
      841 tttggctgga ttctatttcg cgtggattcc atggaaacct ttagaggagt gatgggagga
      901 ctttcgcggc tagagtttag atctcccaat ctgaactgga ttcaaatcct ggttctatgc
      961 gctggatatg gattgcacct ggttccggtg agatggatcc tgcgactgcg cgcaaggtat
     1021 gctctccttc cagtttatgc agctgccact ttatgcgcga tctttctatt gatcgccgga
     1081 cacctggccg ttccgcaagc agtcccgttc atctatttcc agttttgaaa ggcgcctgac
     1141 gtctgcctcc atccttctgc gatcccgcgt gcacggtttt gactgttcgc ccgggagctt
     1201 ctgcggaaaa tggatcatga gcgaacttcg ttttagaact ttatcccagg cggaagaaca
     1261 cctggagcga attgaaaaag caccggctta caaagccacg gggctctggt ctgaagcaca
     1321 aatctttgat cacctcgcaa attcatttga aaattcggtg agcgggtatc caggactact
     1381 ccccgcagtc cttcgcaaga caatcggtag aatgatcttt cgttcccagc aaaagaacgg
     1441 gttcatgaaa cccgggtcgc ccaatccaaa tgcacccaaa gtgcgtgcgg aaggggatgc
     1501 gaagcaggca ttgaaacgtc tgcgaaaggc gatcgtgcag ttcaaagcgc atgacggaga
     1561 gatggccatt catcccgcat tcgatcgcct gtctaaagac gaattctccc acctgcacgc
     1621 catgcacatg gcaaaccatc tggagcatat tcatctagaa gagaccaaag gtcgcgatgc
     1681 gcgaaccgca cgtctgactg gcaaaactca atctggcaaa tcaggatcga gaaagagcac
     1741 gggcaaaaga tccgcgtcta aaaaaacagg aagaagcgcg aagcgaaaat aacactgcaa
     1801 agggttgacc gttttcccgg ttgaccctgt cctgatccat gctggaactc ctgggcgcgg
     1861 ctgcgtctgc gattggctct attgcgtccc agccgtgggc cattgcgctt gcgggcattg
     1921 caagcgcggt tggaatcttc ctggtaaaac attttctcac ccgcgccggg gatgacatgt
     1981 atcactggct gcggcggaag atctttggcc gcagaaaagt ggaaagcgag acacagaggg
     2041 agcttgcatc gcgcgaaacc agaaccgccc gcgagaggtt ttcacctttt gaagtggaaa
     2101 cgcctgtgga tcgctattcg gttacagaat acgtggcccg gggcgatatc gcctcgatct
     2161 acggcgggac aaacagatcc gatcgtcagg tcattttgaa agtcgctatg agcacgggtg
     2221 acaacgatct tctgcaaaat gaagcgaaca cccttcgcgc tcttcaccag ggagacaccg
     2281 tatacaacaa acatttgccg gagcttctag accaatttcg cgcttcttcg ggtgaaatcg
     2341 gcctggtgct tgcgagaatt cgagcatctg atcttcgcac agtgcgagag agttatccgc
     2401 agggcattcc acaacaacac atcctctgga tcctgcgaag aactctcgct gccgtgggac
     2461 acgctcacag ccgcgggatc atccatggaa acatagatcc ttctcatata ctcatcagcg
     2521 cgccggacca caatgtgttc gtcatcgatt gggcgtatgc ggttcacaag ccagcggaaa
     2581 cagggcaggg gttcaaggcg atcaatgatc atttctctcc tccagaagtg gcggagaaga
     2641 aacaacctct tccggcttcc gatctctatt ctattggcaa atgcatgatt tatctcctgg
     2701 gtggaaacgt gcagctagac acaatgccgg ataacgtgga tgaacgcatc cagcgattca
     2761 tcaaattctt tactcggaca agcgcgctcc agcgcgccca ggatgcctgg caaatgtatg
     2821 caaaggcaga tgatctgaga gaagaagtat ttggtcctca taaatttttg gagttcaaga
     2881 ttcaggagta atgtatgggc ggcggctatt acagtcagga cattgcaaga gaagcgagat
     2941 cctccggtgg tggcgtcttc tcttaccagg gatatggagt caacgcagcg gatgcgtctg
     3001 ctagaagaga agttcatcca ctcttgaatg tgcatggcaa aaatcgggaa tgcatgaatg
     3061 agactgccat cgtggttgcg ctggacgtta cgcgctcacg cggcaatgat acaaaagtgg
     3121 tctatgaaaa gctgccttta tttcttggac agattgaact gaagggctac gtgaaaggtg
     3181 ccgcgatcag ctttgctgcc attggagatg ccaattcgga tcaggctcct ttacaggtcg
     3241 ggcagttcga ggcggacaat agactcgacg aagtgctcag caagttctgg atagaagaag
     3301 gcggtggcgg aaccgggcaa gaatcctatg agctcgccgc gtactactac gcgaagcatt
     3361 ccattctaga ttgcaatcgc aagggcaaga aggggttttt cttctttgtt ggggatgaag
     3421 gtttttatcc gagtgtgagt ccggatcatg tagaaaaaat attgggcaga agcatttcag
     3481 gacccgttcc ttctcttcaa gcgtttcagg cactgcaaga gaagtatcat gtgttctttg
     3541 ttcagccaga gaaaacatgg gaagaaagaa aggcggatgt ggacgctgaa attcaggcca
     3601 gagtggaagc agccggcggc cagtataaga acgtgggtgt tcgcgcatcc ctcatctgga
     3661 acaatagaaa tgatctggac ttgcatgtga tctgtccttc tggagaagag atcttctacg
     3721 gccacaagca atccgtatgt ggtggctggc tggacgtgga tatgaatgtt cagggcgaaa
     3781 ccacgaaacc agtggaaaat gtaagatgga gtcgtggcac ggcaccaaag ggaagataca
     3841 ggatattcgt tcagaactat cgaattcacg agggctccgg tccaaccgac tatcgcgttg
     3901 aaattgaaat caatggagaa atcaaacaat tcaaaggaaa gatgtccgcg cacggagaaa
     3961 ctagcgtgga gagcaaccag atcatctatg agttcgacta tgatccatcc aaacgcaaag
     4021 tgcaggatga tcccggagaa aaggcaacgt acgcggcgta tgatgattca gtgattcgaa
     4081 agcaatgggc gagcgtactg ccgtcgtcgc atatcttgcc catcgcagat ccagcaagaa
     4141 ttctggacgt gatgcttggc gcgatggcca tcaccgcggg cacggcagat atttcttctt
     4201 tcgcacggga tttgaagggg agagaccaca cagatgcgca ggtagaggaa gtgcgaaatg
     4261 cattgtccgg aatctccata ccatccgccg gcggctctca gttccccggt gcaggagagg
     4321 gagaaaacag acaatctggt tcgcgtagaa tttagtggcg cgcgcagtac ttctggcagg
     4381 gcttcaattt ggcgatgaag gcaaaggttc cgtaacagac ttcctgtgca gtcgtggcta
     4441 caaccttgta gtgcgataca acggcggtcc tcaggcggcg cataacgttg tattctggcg
     4501 aaatggacag cgcgttcatc attgctttt
//
LOCUS       DAORFF010000305         4527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11073_length_4527_cov_3.993292, whole genome shotgun sequence.
ACCESSION   DAORFF010000305 DAORFF010000000
VERSION     DAORFF010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11073_length_4527_cov_3.993292"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PKK76_12290"
     CDS             <1..550
                     /locus_tag="PKK76_12290"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69302.1"
                     /translation="AKSVFLPEARKIKFDAGSPLPAFYQVTVGGFDEKLPAPYANGIE
                     ISREFTDQSGNKIQSAEAGQEVYVRIRIRAKDTDASNIAIVDLFPGGFEPVFDEKTRT
                     DTVGIPGFKPALMEPDHVEMREDRIVLFTRATSRASEYTYRLRAVSAGKFVVPPLFAE
                     AMYQKSTRALTGEGQFEIKTRK"
     gene            547..2856
                     /gene="pbpC"
                     /locus_tag="PKK76_12295"
     CDS             547..2856
                     /gene="pbpC"
                     /locus_tag="PKK76_12295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNL69303.1"
                     /translation="MTDFIRAFWKRRSKIFKRAAIGAACYVLLRIVSAGGPLSGMPFS
                     RAVYDADGKLLRLTISTDQKYRKFTSLADMSPAVSEAFLLREDKYFYFHPGVNPAALL
                     KATRGYFSGSSARGASTITMQLARMKYGLETRSIRGKIFQILCAFYLEFRYSKSSILE
                     AYLNLIPLGHNIEGVPAASLIYFGKASSSLNPVEAITLCIIPPSPAKRTRNSSNTAME
                     IDQFRKQLFEQWIQKHPEDKELILSVGHATLPRDPLPFLAPHFSQMVLADPSNTDRSE
                     IKTTLRASLQQTLERTISNYVQSRKSTGIKNAAALLVDVHTGKTLAHVGSANFADESI
                     DGQVDGTRALRSPGSALKPLLYALAVDRGIIHPRTLLKDTPIQFSTYSPENFEGEFLG
                     PLSAQSALVLSRNVPAIYIAAKLRQPDFTDFLKSSGVSLSKPKDYYGLAPILGGIEIS
                     MAQLASLYGALAKGGVQRPVSYDGRTNAGKTIFSPEAAFITLEMLKSAREDGMAEEWM
                     QSPFTIYWKTGTSSGFRDAWTAGIFGSYVLVVWIGNFDGSSNPAFVGRDAALPLFLRI
                     ARAMAAENNSIKDWIEAPAGVSRIEVCSVSGKIPRNFCPLRAHTWYIPGKSPIEPCDI
                     HREVAVDLDTGLRACAGAKNTKKEVYEFWPSDIERVLKSAGLPHKAPPRFSPECQTAD
                     NHAEPPSIHSPVHGVTYESSREGEEIALEAAADASVTDLFWFVDDGFVGKSKPGESLF
                     WRAHPGEFKIRVVDSGGASDQMTVKINAR"
     gene            2905..4413
                     /locus_tag="PKK76_12300"
     CDS             2905..4413
                     /locus_tag="PKK76_12300"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HNL69304.1"
                     /translation="MKGKTLGFLRLTGILLRSAPFALDVVMEKSRQNPRPGFIQACLR
                     SMDGLFRALFPDASAARPHRLLRLLIQLGPTFIKIGQAMANRPDLVPLSYMHELESLQ
                     DRVPPFATWRAKRILRKELKRKGKTLSVFTVFPKTSIAAGSLGQVYECFLDDGTRVAV
                     KIQRPGIDKKIARDIEALRILARYAASHTGIGMGVNWDDVVEEFADTIRMELDFGREM
                     ENAEKFRDNFKAWKDIYVPIFYPEYCTKKVLVMEFIDGLKLNDLPALSAADKDPMDGV
                     RIVTRSYLKQLIQDGFFHADPHPGNLRLLEDGRVAFFDFGMAGSIDDEQKSMVLDLLI
                     HVAEQDIHAIVQDLANLGFLRTGHDFSAFQPAIEEVFRHYLGETERDRIQIHEIVYAM
                     SEIVYRYPFTIPTRFTYILRALLTLEGIGLSITPAFSFFRFARAYALDYFFLSQMGIL
                     AKKLLRGLIGSADLKDGWDKTKKFSRMLWNYVTNKNVLNLHLEGPEDPSEKQ"
BASE COUNT         1203 a         1198 c         1092 g         1034 t
ORIGIN      
        1 tgcaaaatcc gtcttccttc cggaagcgcg caagatcaag ttcgatgcag gatcgccgtt
       61 gcctgcgttc tatcaggtca ccgtgggcgg attcgatgaa aaacttccag caccgtacgc
      121 aaacggaatc gaaatttccc gcgagtttac agatcaaagt ggaaacaaga tccaatctgc
      181 agaggccggc caggaagtgt atgtaagaat ccgaatccgc gcaaaagaca cggatgcatc
      241 caacattgcc attgtggatc ttttccccgg aggtttcgaa ccagtcttcg acgaaaaaac
      301 aagaacagac actgtgggca ttcccgggtt caagcccgca ttgatggaac cagatcatgt
      361 ggaaatgcgc gaagacagaa ttgtactttt cacaagagcc acatcacgag catccgaata
      421 tacatacaga ttgcgcgcgg tgagtgcggg caagtttgtt gttccacctc ttttcgcaga
      481 agcaatgtat caaaaatcta ctcgggcgct cactggagaa ggacagttcg aaatcaaaac
      541 caggaaatga cggatttcat tcgagccttc tggaagagaa ggtcaaagat tttcaagcgc
      601 gcagcgattg gcgctgcgtg ctacgttttg ctcaggattg tttctgccgg tggacctctc
      661 agcggaatgc ctttctcgcg cgcggtctac gacgcagacg gtaagctact tcgcctgact
      721 atttccacgg atcaaaagta tagaaagttt accagtctcg cagatatgtc gcctgccgta
      781 tccgaggcat tcctgttgcg cgaagacaaa tacttttact ttcacccggg agtgaaccct
      841 gccgctctgc tcaaagcaac aagaggttat ttcagcggca gttctgcgcg cggtgcttcc
      901 acgattacca tgcaactggc tcgaatgaag tatggtctcg aaaccagatc gattcgcggc
      961 aagatctttc aaattctatg cgcattctat ctagaatttc gttattcaaa atcgtctatc
     1021 ctggaagcat acctgaacct gattccactc ggccacaata tagaaggagt cccggctgct
     1081 tccttgatct actttggaaa agcgtcctct tctctcaatc ctgtggaggc aatcactctt
     1141 tgcatcattc ctccaagccc ggcaaaacga acccgcaact caagcaacac tgccatggag
     1201 atagatcagt ttcgcaaaca actctttgaa caatggatcc agaaacaccc tgaagacaaa
     1261 gagctcatcc tctctgtggg tcacgcaacg cttccgcgag atcctcttcc ttttctcgca
     1321 ccgcattttt cgcaaatggt cctggcagac ccctcgaata cagaccgatc agaaatcaaa
     1381 accactctgc gcgcaagtct gcaacaaact ctggaaagaa cgatttcgaa ctacgtccag
     1441 tcgcgaaaat cgactggaat caagaatgcc gccgctttgc tcgtggatgt gcatacagga
     1501 aagacactcg ctcatgttgg aagcgccaac ttcgcagacg aaagcattga tggacaggtg
     1561 gatggaactc gcgcactgag atctcctgga tccgcactga agcctcttct ctatgctctg
     1621 gccgtagacc gtggaatcat tcatccgaga acactgttga aagacacgcc tatccagttc
     1681 tcgacctaca gtccggaaaa ttttgaagga gagtttttgg gcccgttatc tgctcaatcg
     1741 gcgctggttc tgagcaggaa tgtgcctgcc atctacatcg cagcaaaact cagacaacca
     1801 gacttcacag acttcttgaa atcatctgga gtatctctat ccaaaccaaa ggattactat
     1861 ggcctcgctc ccattctggg cggaatcgaa atctcaatgg cacagcttgc gtcactctac
     1921 ggagctcttg ccaaaggagg agtgcagaga ccagtctctt acgatgggag aacgaatgca
     1981 ggaaaaacca tcttcagccc agaagctgca ttcattacac tggagatgct caagtccgca
     2041 cgcgaagatg gaatggcaga agaatggatg caatcgccat tcaccatcta ctggaaaaca
     2101 ggaacgtcat ccggctttcg tgacgcctgg actgcgggaa ttttcgggtc ctatgtcctg
     2161 gttgtgtgga tcgggaactt tgacggttcc tccaatcccg ccttcgtagg gcgagacgcg
     2221 gcccttcctt tgtttctgcg aatcgcgcgc gcaatggccg cagaaaataa cagcatcaaa
     2281 gattggatcg aagctcccgc cggagtgagc agaattgaag tgtgttcggt ttccggaaaa
     2341 attccgagga acttctgccc gcttcgcgcg catacatggt acattcctgg aaagtctccg
     2401 atcgaacctt gcgacataca ccgcgaagtc gcagtagatc tggacaccgg actgagagca
     2461 tgcgccggag caaaaaatac aaagaaagaa gtttatgagt tctggccgtc tgacatagaa
     2521 agagttctca agagtgctgg cctgccgcac aaagcgcctc cgcgattttc ccccgaatgc
     2581 cagactgcgg acaaccacgc agagccaccc tccatccatt cgcccgtgca tggagtgacg
     2641 tatgaatctt cacgcgaagg cgaagagatc gctcttgaag cagccgcgga tgcatccgtc
     2701 acagatctat tctggtttgt agatgatggc tttgttggaa aatcaaagcc gggagagtct
     2761 ctcttctggc gcgctcatcc aggggaattc aagatacgag ttgtggactc aggaggcgcc
     2821 agcgatcaaa tgacagtcaa aatcaacgcg cgatgatccg caaaaatcga ttgcactcct
     2881 tattccccgc tccgagctac cagaatgaag ggcaagaccc tcgggttcct gcggctcact
     2941 ggaatacttc tgagaagcgc tcctttcgcg ctcgacgtcg tgatggaaaa atcacggcaa
     3001 aacccccggc ccggattcat tcaggcatgc ctgcgaagca tggacgggct ttttcgcgcg
     3061 cttttccctg atgcgagcgc cgccagacct catcgccttt tgcgccttct cattcagctg
     3121 ggaccaactt tcatcaagat aggccaggca atggccaacc gcccggacct ggttcctctt
     3181 tcatatatgc acgaactgga aagtctccag gaccgcgtgc cgcctttcgc aacctggcgc
     3241 gcaaagcgca ttctaagaaa agaactgaaa cgaaaaggaa agacactgag cgttttcaca
     3301 gtttttccaa aaacatcgat cgcagccgga agcctgggcc aggtctacga atgctttcta
     3361 gacgatggaa cccgggttgc agtgaaaatc cagagaccgg gaatagataa aaaaatcgcg
     3421 cgggacattg aagcactgag aatacttgca cgttatgcgg catctcacac cgggatcggt
     3481 atgggagtaa actgggacga cgttgtagaa gaattcgcgg acacaatcag aatggaactg
     3541 gacttcggtc gcgaaatgga aaacgcagaa aaattccgcg ataacttcaa agcctggaaa
     3601 gacatttacg ttccaatttt ctacccggag tactgtacaa agaaagtgct cgtgatggag
     3661 ttcatagacg gacttaaact caacgatctt cctgcattga gtgcagcaga caaagatccc
     3721 atggacggtg tgcgcatcgt cacgaggtcg tacctcaaac agttgatcca ggatggcttc
     3781 tttcatgcgg acccgcatcc tgggaattta cgcctcctcg aagatgggag agtggcattc
     3841 ttcgattttg gaatggcagg aagcatagac gacgaacaga agtcgatggt gctggatctg
     3901 ctcattcatg tggcagaaca ggacatccat gcaatcgtgc aagacctggc caatctgggc
     3961 tttcttcgca ctggccacga cttcagcgct tttcaacccg cgattgaaga agtattccgt
     4021 cactacctgg gtgaaacaga gcgcgacaga attcagattc acgaaatcgt ttacgccatg
     4081 tcagaaatcg tctatcgtta tcctttcacc attccaacgc gattcaccta catcctgcga
     4141 gcgctcctta ccctggaagg aatcggactg tccattaccc ctgctttcag tttctttcgc
     4201 ttcgcacgag cttatgcact ggattatttc ttcttatctc aaatgggaat tctcgccaaa
     4261 aagctgttgc gtggtctgat cggatcagcg gacctcaagg atggctggga caaaacaaag
     4321 aaattttcgc gaatgctctg gaactatgtg accaataaga atgtactgaa ccttcacctc
     4381 gaaggcccag aagatccctc ggagaaacaa tgaacggaat cgccacatat ctaagaatga
     4441 tcaagttctc gcatacgatc ttcgcgcttc ctttcgcggg cattgcattc attcaggcac
     4501 tgcccgcctc acagttgatc tcaaacg
//
LOCUS       DAORFF010000306         4498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11177_length_4498_cov_3.883862, whole genome shotgun sequence.
ACCESSION   DAORFF010000306 DAORFF010000000
VERSION     DAORFF010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11177_length_4498_cov_3.883862"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PKK76_12305"
     CDS             <1..919
                     /locus_tag="PKK76_12305"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNL69305.1"
                     /translation="DGGIARVPGTNKGISVSTDCNSRYVFLDPYKGAAQAVFESARNV
                     AVTGAEPLGITNCLNFGNPNIPENYYVFSECIRGMGDACRSMGIPVTGGNVSFYNESE
                     DGPVFPTPTIGMVGLVEDIGRAVFPWFSSPGDLIFVAGHFAPSFGGSEYLKLNTGKSA
                     GQTADLNFDHEKQLIQFLLQGAKRRLFASARDLSIGGLSAALARSVLSRNFDPRATSS
                     PRGFALSIAEPYKSDLDLTLFGETNGCAIVTVKPGSEAQTEALAKASGIPLWKIGTVS
                     SDAMFNYGGFQISYEEAAAAYEGGLAGVF"
     gene            complement(922..1305)
                     /locus_tag="PKK76_12310"
     CDS             complement(922..1305)
                     /locus_tag="PKK76_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69306.1"
                     /translation="MPQSRLTLTILITAISLVDCRTVETGTEARVVDLRIAGNTDGVT
                     IHRMDPGGEKAVLRTDIRYYKLDLPAMQGGYSEALFLIRYNIHDARTYPIVRILRDGT
                     SIQTLSIQDLQNLPEDQGMRVLTLP"
     gene            complement(1308..2312)
                     /locus_tag="PKK76_12315"
     CDS             complement(1308..2312)
                     /locus_tag="PKK76_12315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNL69307.1"
                     /translation="MSQEDLEKLKKTIEAYDKLSELSHKELLEAHQELQARESQQELS
                     REELIRLHEQVRGLENEKASLETRIRSVLHEDKANEARILSELDALRQESDAGFFVNL
                     FRVLCHHEFEPDAAMTHWADILKHSAEMQAKLGRPIGFRVAMMDYFINLNRVLKSPVI
                     LEIALYDEMMQNALMDELTGVYNRRYFERSLNREINRARRHSHSLSLLALDLDNFKEF
                     NDRHGHAAGDQVLAAFSDQLRKSFRAEDIVCRYGGEEFVVILPLTTPVNAMIVAQRFL
                     DEVRKLEIYGRSVTCSGGIAGYPEHESEPRGLFKAADRALFIAKKRGKDRILFADEVI
                     "
     gene            2366..2728
                     /locus_tag="PKK76_12320"
     CDS             2366..2728
                     /locus_tag="PKK76_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX-like family protein"
                     /protein_id="HNL69308.1"
                     /translation="MNELALYVMSVFYIFAGIMHFAKPKLYLRMMPPWLPAQKAMNYI
                     SGAAEVFLGIGLVPLVTRAWAAWGIIALLVAVFPANVYMLTSGKFKLPKPLLILRLPL
                     QLVLIWWAYQYTGQSLTR"
     gene            complement(2710..4212)
                     /locus_tag="PKK76_12325"
     CDS             complement(2710..4212)
                     /locus_tag="PKK76_12325"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL69309.1"
                     /translation="MKNRYYLSMLGLFPFIIVIDGIYFVYHQSITIFLGLGFVHLLLY
                     GLINYIGARLLYRPVDRLFEGTGDLPAARKRIEQLTWQSTAWIFLIGNIYVAIALLPL
                     YFFPRIFQNPEDFVIDKIPPQFFFFSIVPGIYFVYALFPSFIAYFLINDFILDLKETV
                     SARFKMLFDAGSKGIGRTLFFVFLFLVFLPSLLVILELKVAFQVEDKYAQFTKLSPLQ
                     TVLIDRFIVFLGTIVAVVLVTRTFTKPIRSLIKAINRVREGDYSTQAAVITEDEIGLV
                     TQEFNGMVRGLQERELIRDTFGKYVTRDVATVILEEKIDLAGEVRTATILVTDIADYT
                     TISETLTPREIVMMLNEYFSELVEIIHAHRGVVNKFIGDAIFAMFNVPLDDPNHAANA
                     IRAALAIEKVTSSRTFGKDRKLRTRIGINTGVVVAGNIGSADRLEYTVIGDDVNIAAR
                     LEQLNKEHGTSVLLGENTYELAKDQFKFKELGGFQLKGKEKIIQVYRVSD"
     gene            complement(4217..>4498)
                     /locus_tag="PKK76_12330"
     CDS             complement(4217..>4498)
                     /locus_tag="PKK76_12330"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNL69310.1"
                     /translation="DSGAKVEIVEYLKNPPTATELSAILDKLGMKAEDLIRKGETVYA
                     EKYKGKTLTEKQWIQAMVENPILIERPIVVSGKKAVLGRPPENVKKLLR"
BASE COUNT         1103 a         1110 c         1086 g         1199 t
ORIGIN      
        1 ggacggtgga atcgcgcgcg ttcctgggac aaacaaagga atttcagtat ccacggactg
       61 caacagcagg tatgttttcc tggatccgta caaaggagca gcgcaggctg ttttcgaatc
      121 ggccagaaac gttgccgtta ccggagcgga gcctcttgga atcacaaact gcctgaactt
      181 tggaaacccg aatatcccgg agaattacta cgttttcagc gaatgcattc gcggcatggg
      241 ggatgcgtgc aggtccatgg ggattcctgt aacaggagga aacgtttcct tctataatga
      301 aagcgaagat gggccagtat ttccaacccc aaccattggg atggttggtc tggtagaaga
      361 cattggacgc gcggtgttcc cgtggttttc ctctcccgga gatttgattt tcgtcgctgg
      421 tcattttgct ccgtcctttg gcggaagcga atacttgaaa ttgaatacgg ggaaatccgc
      481 cgggcaaaca gcggacctca atttcgatca cgaaaaacaa ctcattcaat ttctgctgca
      541 gggcgcaaag aggagactct ttgcatctgc gagagacctt tctatcggtg gactttctgc
      601 cgctctcgca agatccgttc tttcgcgcaa ttttgatcca cgcgccactt cttctccccg
      661 tggctttgca ctgagcatcg ccgagcctta caaatcggac ctggatttaa cgttattcgg
      721 cgaaacaaac gggtgcgcga tcgtaactgt gaagcctgga agtgaagcac agacagaggc
      781 cctcgcaaaa gcatccggaa ttccgctgtg gaaaatcggc actgtgtctt cagatgcaat
      841 gtttaactac ggtggctttc agatcagtta cgaagaagcg gccgcggcct acgaaggcgg
      901 actggctgga gttttttgag atcagggcaa ggtgagtacg cgcattccct gatcctccgg
      961 tagattctgc agatcctgaa tggaaagtgt ctggatggat gttccatctc gcaggattcg
     1021 aacgatcggg taggttcgcg cgtcgtgaat attataacga atcaggaaca gtgcttctga
     1081 ataacctcct tgcatggcag gcaggtccag tttgtagtaa cgtatgtccg ttctgaggac
     1141 tgctttttca ccacccggat ccattctgtg aatggtcacc ccgtccgtgt ttcccgcaat
     1201 cctcagatcc acgactctgg cttccgtgcc agtctccacg gttctgcaat cgacgagcga
     1261 gatcgcggta attagaatgg tcaacgttag gcggctttga ggcaacctca gattacctcg
     1321 tccgcgaaca gaattctgtc cttgcctctc ttttttgcaa tgaacagagc ccgatccgct
     1381 gccttgaaca ggccgcgtgg ttcactttca tgttcgggat aacctgcaat tccaccgctg
     1441 caggttacac ttctaccata gatttcaagc tttcgcactt cgtcgaggaa ccgctgcgct
     1501 acgatcatcg catttactgg agtagttaat ggaaggatca caacaaattc ttctcctccg
     1561 tatctgcaaa cgatgtcttc tgcgcggaaa ctctttctga gctgatccga gaatgcagcc
     1621 agaacctgat ccccggccgc gtggccatgc ctgtcattga attctttgaa attgtcaaga
     1681 tccagagcga gcaaagaaag gctgtgggaa tgacgacgtg cgcggttgat ttcgcgattc
     1741 agcgaacgct cgaagtagcg ccgattgtag accccggtca attcatccat gagtgcgttc
     1801 tgcatcattt cgtcatagag tgcgatttca agaatcaccg gacttttcaa aacgcgattg
     1861 agattgatga agtagtccat catggccaca cgaaagccaa tgggccgacc aagcttagcc
     1921 tgcatttccg cagagtgttt caaaatgtct gcccagtgtg tcatggccgc atctggttca
     1981 aattcgtgat ggcacaacac tctaaacaga ttcacgaaga atcccgcgtc ggactcctgt
     2041 ctgagtgcgt ctagttcaga aagaatgcga gcctcatttg ccttgtcttc atgcaagaca
     2101 ctgcgaatgc gagtctccag actggccttt tcattttcaa ggcctcgcac ctgttcgtgc
     2161 aggcgaatga gttcttcgcg ggataactcc tgctgagatt cgcgcgcctg gagttcctga
     2221 tgggcctcca gtagctcttt gtggctcagt tcggacaatt tgtcgtaagc ttctatggtc
     2281 ttttttagct tttctaagtc ttcctgactc atgtgcagac tcttttttgc tacctgaaat
     2341 tgaccatcca ctttttgtta ggcgaatgaa cgaactggct ctctatgtta tgtccgtatt
     2401 ctatatcttt gcgggcatca tgcacttcgc gaaacctaaa ctttatttaa gaatgatgcc
     2461 tccctggctt ccagcgcaaa aggctatgaa ttacatcagc ggcgcggcag aagtttttct
     2521 gggaattggc ctggtaccac ttgtaacgag ggcatgggcc gcctggggaa tcattgcact
     2581 tctggttgcg gtgtttccgg ctaacgtcta catgctcaca agcggaaagt tcaaattgcc
     2641 aaagccgctt ctgatcctgc gactcccact ccagcttgtt ctcatctggt gggcctatca
     2701 gtataccggt caatcgctta cgcgatagac ctgaatgatc ttttcttttc ccttgagctg
     2761 gaagccaccc agctctttga atttgaactg atcttttgcc agttcgtatg tgttctctcc
     2821 gagtagaacg gaagtgccgt gttccttgtt cagttgctcc agtctggctg ctatattgac
     2881 gtcatccccg atgactgtgt attcgaggcg atccgccgat ccaatgttgc cggctacaac
     2941 cactcctgta ttgattccaa ttctagttct gagctttctg tctttgccaa acgtgcgact
     3001 ggacgttacc ttctcaatgg caagagctgc gcgaatcgca ttggctgcgt gattgggatc
     3061 atccagtggc acattgaaca tggcaaagat ggcatccccg atgaacttat tgaccactcc
     3121 cctgtgagcg tgtatgattt ctacaagttc agaaaagtat tcgttgagca tcattacgat
     3181 ttcgcgcgga gtgagcgttt ccgaaatcgt ggtatagtct gcaatatcgg tcaccagaat
     3241 tgtggccgtg cgcacttcac ctgccagatc aattttttcc tcaaggatca cggtcgcaac
     3301 atctcgagtc acatattttc cgaatgtatc tcgaatgagc tcacgctcct gcaggcctcg
     3361 aaccatcccg ttgaattcct gggttacaag gccaatttcg tcctccgtga tgacggcggc
     3421 ctgagtagaa tagtctcctt cgcgtacccg gttgattgct ttgatgaggg atcggattgg
     3481 tttggtgaag gtgcgtgtaa caagcactac agcaactatg gttcccagaa atacaatgaa
     3541 gcgatcgatc agaactgttt gtagagggct gagctttgta aactgggcgt acttatcctc
     3601 gacctgaaac gcaaccttga gttcgagtat gaccagtaag gatggaagaa acacaagaaa
     3661 cagaaaaacg aaaaacaaag tccgtccaat ccctttgctc ccggcatcaa agagcatttt
     3721 aaagcgcgcg cttacagtct cttttaaatc gagaatgaaa tcgttgatca agaaatacgc
     3781 aatgaacgac ggaaagagag cgtacacaaa ataaattcca gggacaatgg agaaaaagaa
     3841 aaactgcggc gggattttat ctatgacaaa gtcttccgga ttttgaaaga tacgcggaaa
     3901 aaagtagaga ggcaaaagag caatcgccac ataaatgttt ccaatcagaa aaatccacgc
     3961 agtagattgc caggtcagtt gttcgattct ctttctggct gcgggcagat ctccagttcc
     4021 ctcaaacaaa cgatccacag gtctgtagag tagacgcgcg ccgatgtaat taataagccc
     4081 gtacaaaagc aaatgcacaa agccaagtcc gagaaaaatc gtaatggatt gatgatagac
     4141 aaaatagatt ccgtctatca cgatgataaa cggaaagaga cccagcatcg aaagatagta
     4201 gcgatttttc attcgatcat cgcagaagct ttttcacatt ctccggcggc cttccaagga
     4261 ctgctttttt tccagacact acaatcggac gctcgattag aatcgggttt tccaccattg
     4321 cctggatcca ctgtttttcc gtaagagttt tgcctttgta tttctctgcg taaacagttt
     4381 cccctttgcg aatcagatcc tctgctttca ttccaagttt gtcgagaata gcagacagct
     4441 cggtggcagt gggcggattc ttcagatatt caactatttc tacttttgcg ccggagtc
//
LOCUS       DAORFF010000307         4489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11220_length_4489_cov_3.258683, whole genome shotgun sequence.
ACCESSION   DAORFF010000307 DAORFF010000000
VERSION     DAORFF010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11220_length_4489_cov_3.258683"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PKK76_12335"
     CDS             complement(<1..642)
                     /locus_tag="PKK76_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004752634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNL69311.1"
                     /translation="MASDSKIIPTLDNPFEEAPAQAAEPSSNLNLLAYEKTLEMGKDL
                     LAKPLKGGGVSRIMVQHSKHRMTVFERIKVLTESEPNILFQNWGRELDGAGLVTGILR
                     VGNRDVAIYGHDFTNRAGSMDATNGAKLARLIYLAGEQGIPLIGLNDSAGAYVPAGVG
                     GLDGYSEAFCALRKISGVVPSIMVMFGFNAGGGSYLPRQGSFMIQPHDTFFGLT"
     gene            697..2232
                     /locus_tag="PKK76_12340"
     CDS             697..2232
                     /locus_tag="PKK76_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69312.1"
                     /translation="MEGQRGKLKALVWLAIAGLCIFGVSRLDRAVCFSGDSLSKLVQA
                     ESLRKSGFSSEELFYPGRAIDSEYRFYPFPGVYHLIIHGRHLGQYPIAFAAATGLLLK
                     AISPGGLFILGAALYLLLMYLFDRFAQPSPLLLLFVAAGTPILTFSVDYSEHIYFALI
                     NFGGLILFLSGLRLENSRRLFLGGMLIGFGLYLRLESLIFFSALFGGVCLELILSRRL
                     TFKSLQPPGLFAGGFLVAAGSFFLFNALHYGHPLGPRFMANEEAFAIPLASRLGQIIT
                     LLFARGFQLGFFGYMPIFLFVLGATLLPAFRSRLDFANRSMALAMIFYLPVVAVASPG
                     DGVVNWGPRFLLLAILPCALLLVSLSRFTWPAIVRAGAVLLCVVSIAFTFMGMRLQKN
                     ACRQLSQYQNDFVNAQADIWIFSSQLLSNYMGPEFFARETYLAQDAESARELAKKLRS
                     SSKKPLRVAYFESVVPEEWKKALAEQNLVDRPEVGAELKKDLRATGEVKGRFTLATIF
                     EIP"
     gene            2229..3683
                     /locus_tag="PKK76_12345"
     CDS             2229..3683
                     /locus_tag="PKK76_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69313.1"
                     /translation="MTRRSNSMKRFFQATFLLVLSGGALYARPSTTVTGETKRDYYEF
                     LFLFDKTSAPGQSEWILHPFASRYSNMERSYTFTTILYPIFYSHGTYRWNRWTFLYLF
                     TGDDIYHDDSGLEEDNFITPILYWGTGETERERYFSVFPFYGRIKNKLSYSEINYVMF
                     PLYSGWSHKDYSAHGILWPIVMWGGSPTRSDFRIFPFFSRKIHTGKYERYSALWPIFQ
                     WGVEGLDQKDPRHMFMVFPFYGRKWSESGQMTAHTVLWPLFSWGHDDATNGFDLNLFW
                     FLFQYQVNDSPLIRKLIIFPFYGRYVFGSKGSLTDQSGIYSQEYSFVTPFYATMRTSS
                     ALLESNSKYVVPVFMYDQTYYRKERETETYWKIWPLVSTSSDSNGRFEFRSLSLWPFR
                     SDEFERIWGPLYSVVTYRRDENEEKYFSVLFRLYSRKWSSDEEHHFFVGFNYDNTPDV
                     TGVEFLGGFLGFRREYNREDRETKNRVRLLWFSI"
     gene            3738..>4489
                     /locus_tag="PKK76_12350"
     CDS             3738..>4489
                     /locus_tag="PKK76_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNL69314.1"
                     /translation="MKQIKGLVERILRPILGAGETLLLLFRSVLQLPFLWFKRNDIIR
                     QMYVSGFKSLSVVSVVATFTGMIFSLQTGLALKDFGQQDLIGQVIVVTLTREMSPFMT
                     ALILAASVGSAIAAEIGTMTVSEEIDALEVMSIDPVRYLVMPRIVGFSLMVPVLSIYA
                     TLLGILGGGLVAQTQLGVDFSVYFQTVYDSLLTKTGLKDVWVGQLKAIVFGITISTIS
                     CRQGLAASGGAIGVGIAVRQAVVISFLFVIILG"
BASE COUNT          929 a         1087 c         1178 g         1295 t
ORIGIN      
        1 ggtcagacca aagaaagtat cgtgcggctg gatcatgaac gatccctgcc gtggtagata
       61 cgatccaccg ccagcgttga atccaaacat caccatgatg ctcgggacca ctccagaaat
      121 cttgcgaaga gcgcagaacg cttccgagta tccgtccaga cctcccacac ctgcaggaac
      181 ataggcgccc gcagaatcgt tgaggccgat gagtggaatt ccctgttcgc ctgccaggta
      241 gataagtcga gcgagctttg ctccgtttgt agcatccatg gagcccgcgc gattggtgaa
      301 gtcgtgtccg tagatggcca cgtcgcggtt ccccactcga aggatgcctg tgacgagtcc
      361 tgcgccgtcc agctcgcggc cccagttttg aaagaggatg tttggttctg actcagtgag
      421 tactttgatg cgctcgaaga cggtcatccg atgcttggaa tgctgcacca tgatgcgcga
      481 aacgccgccg cccttcagtg gctttgccag aaggtctttg cccatttcca gggtcttttc
      541 gtatgccagt agattcaggt tggaagatgg ttctgcggcc tgggccgggg cctcttcaaa
      601 gggattgtcc agagttggaa tgattttgct gtcggatgcc atctcgattg aatttttact
      661 ttctccgcgg gtgtcgagta aaaatggtct attatcgtgg aagggcaaag agggaagctg
      721 aaggcactgg tatggcttgc gatcgcaggc ctttgcatct tcggagtttc gaggctggat
      781 cgagctgttt gcttttcagg cgacagtctg agcaagcttg ttcaggcaga gtctcttcgc
      841 aagagcgggt tttcctcaga agagctcttt tatcccgggc gtgccataga ctcggagtat
      901 cgcttttatc ctttccccgg tgtgtatcat ctaatcattc atggcaggca cctgggccag
      961 tatccaattg cgtttgcggc cgccaccggt cttctcctca aagcgatttc tccgggcggc
     1021 ctctttattc tcggggcggc gctctatctg cttttgatgt atttgtttga tcgctttgcg
     1081 caaccgtctc ctctgcttct actgtttgta gctgcgggca ctccgattct cactttcagc
     1141 gtggattact cggaacatat ttactttgcc ctcatcaatt ttggcggact gattctcttt
     1201 ctgagcggac taagacttga aaactcgcgt cgattgtttc tgggcggaat gctgatcggc
     1261 tttggattgt atcttcgcct ggaatcgttg attttcttct ccgctttgtt cggtggagtg
     1321 tgtcttgagc tcattctgag tcgtcgccta acgttcaaat cgctccagcc tccgggtctt
     1381 tttgccggag gttttctggt ggctgccgga tctttctttc tgttcaatgc gcttcactac
     1441 ggtcatccgt taggacccag attcatggca aacgaggaag cattcgcgat tcctcttgca
     1501 tccaggttgg gtcaaatcat caccctgctt ttcgcgcgcg gctttcagct cgggtttttt
     1561 ggctatatgc ccatctttct ctttgtgctg ggtgccactc ttcttcccgc attcaggtct
     1621 cgtcttgatt ttgcgaatcg ctccatggcc ctggccatga tcttttatct tcctgtggtg
     1681 gcagtggcat ccccggggga tggagtggtg aactggggcc ctcgcttttt acttctggcc
     1741 attcttccgt gcgctctctt gctagtgtct ctgtccaggt ttacgtggcc tgcgatcgtt
     1801 cgcgcgggcg cggtgcttct gtgcgtggta tccatcgcgt ttactttcat gggaatgcgc
     1861 ctccagaaga atgcatgcag acagctttcg cagtatcaaa acgattttgt aaatgcgcag
     1921 gcggacatct ggattttttc aagccagctt ctttcaaatt acatggggcc ggagtttttc
     1981 gctcgcgaaa cctatctggc acaggatgct gaatcagctc gcgagctcgc aaaaaagctc
     2041 aggtcttctt caaagaagcc ccttcgagtt gcgtattttg aatcggtcgt gccggaagaa
     2101 tggaagaagg cactggcaga gcagaacctg gtcgatcgcc cggaagtggg agctgaactg
     2161 aaaaaggatc tgcgcgcaac gggtgaagtg aaaggtcgct ttacactcgc aactatcttt
     2221 gagattccat gactcgcaga tccaattcaa tgaagagatt ctttcaggcc acgtttcttc
     2281 tggttttgtc aggaggtgct ctttatgcgc gcccatctac caccgtgacc ggagaaacaa
     2341 agagagacta ctacgaattc ctgtttctct tcgataagac atccgcaccc ggtcagtctg
     2401 agtggatttt gcatccgttt gcaagcagat actccaacat ggagagatcc tacactttta
     2461 caaccattct gtatccaatc ttctattcgc acggaacgta cagatggaat cgctggacat
     2521 ttctgtattt gttcaccgga gatgatatct accacgatga cagcggactg gaagaggata
     2581 actttatcac gccgattctt tactggggca cgggtgagac agaaagagaa aggtatttca
     2641 gtgtatttcc attttatggt cgcatcaaga acaagctgag ctattctgaa atcaattacg
     2701 tcatgttccc tctgtactcc ggctggtcgc ataaggatta cagtgcgcac ggaatcctct
     2761 ggcccatcgt catgtgggga ggcagtccca cccggagcga ttttagaatt ttccctttct
     2821 tttctcgaaa gatccatacc ggaaaatatg aacgttattc ggcattgtgg cctatttttc
     2881 aatggggtgt tgaggggcta gatcagaaag acccacgaca tatgttcatg gtattcccgt
     2941 tttacggtcg caaatggtcc gagagcggtc aaatgacggc gcatactgtt ctctggccct
     3001 tgttttcatg gggacatgac gatgcgacga atggctttga cctgaattta ttctggtttc
     3061 tgtttcagta tcaggtgaat gattctccgc tgattcgcaa gcttatcatt tttccgtttt
     3121 acggtcgtta tgtgtttggt tcgaaaggat ctctcacgga ccaaagtgga atctactctc
     3181 aagaatacag ttttgtgacg ccattttacg ccaccatgcg cacgagctct gctctcctgg
     3241 aatccaattc gaagtatgtg gtgcccgtat ttatgtacga tcaaacatac tacaggaaag
     3301 aacgcgaaac ggaaacctat tggaagatct ggcctctcgt ctccacgtcg agcgattcga
     3361 acgggcgatt tgagttccgt tcactgagtc tgtggccctt tagatcggac gagtttgaaa
     3421 gaatctgggg accgctgtat tcagtcgtga catatcgtcg tgatgagaat gaggaaaagt
     3481 atttttccgt tctgtttcgc ctgtattcca gaaagtggag ctcagatgag gagcatcatt
     3541 tctttgttgg gttcaactac gacaacacac cggacgtgac gggcgtagag tttctgggtg
     3601 gcttcctggg cttcaggcgt gagtacaacc gtgaggacag agaaacaaag aatcgagtgc
     3661 gcctcctgtg gttctcgatt tgaattcgga cttgacatga ccttacgaat agcaactctt
     3721 ccggcgagca cgggcaaatg aaacagatca aaggtttggt cgaacgaatt ctgcgcccga
     3781 tccttggagc cggggaaaca cttctacttc tgtttcgctc cgtgttgcag ctgcccttcc
     3841 tgtggttcaa gcgcaacgac atcattcgcc agatgtacgt ttccgggttc aagagtcttt
     3901 cggtggtcag tgtagtggcc acgtttaccg ggatgatctt cagtttgcag actgggcttg
     3961 ctttaaaaga ttttggtcag caggatttaa tcggacaggt cattgtggtt actctgacca
     4021 gggaaatgtc tccgttcatg actgctctga ttctcgctgc ttctgtcggg tctgccattg
     4081 cagctgaaat tggaaccatg actgtatctg aagaaattga cgcgctcgaa gtgatgtcga
     4141 tcgatcctgt ccgctatctc gtgatgccgc gtatcgtcgg gttcagcctg atggttcctg
     4201 tcctttctat ttatgcaacg ttacttggaa ttctaggcgg aggcctggtg gctcagactc
     4261 agctgggcgt agatttttct gtgtactttc agactgtgta tgattctctt ttaacaaaaa
     4321 cgggtctcaa agatgtctgg gtgggtcagc tcaaggcaat cgtgtttggg attacgatct
     4381 ctaccatatc atgcagacag ggtctggccg ccagcggtgg ggccattggg gtgggcatcg
     4441 cagtgcgcca ggctgttgtg atttccttcc tttttgtgat tattctggg
//
LOCUS       DAORFF010000308         4481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11253_length_4481_cov_3.248531, whole genome shotgun sequence.
ACCESSION   DAORFF010000308 DAORFF010000000
VERSION     DAORFF010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11253_length_4481_cov_3.248531"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1731
                     /locus_tag="PKK76_12355"
     CDS             220..1731
                     /locus_tag="PKK76_12355"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL69315.1"
                     /translation="MSVYRVFINQNDDQLGAISSEVYVVKKGDTLFYVSNHVARGGGI
                     LGYRFGKETDLEAERARSLYQEAMVLGMLNAAGFFFLIYFLARGGGAALPVFTLLMIV
                     CTVRSAASNSVLEEWFPDRQLTDLRLRLEYLTALALMPPLYFWAIALLFQGDPETSLA
                     TLRRTITRILSAVYSIGAFCLSLFFIFARDASVYGKYQPEYVSCYLLPGLAVCLFLLF
                     ILVIEKRRGAGWMLLGFLSVLFSTLADAETTVAGQAGTLYVPLGLLGFAACFSVTVGI
                     RIRSDFEELALTRKNLALSFEQDRRREQKRASEREQIVLQAVSALSRTASDFEEMLSQ
                     GKVQKDAAIPALSRLHSLRVRLEKRLSRTPVAPVALDVLRYIRFHHPSVRIPSQASLV
                     VFSSRRELDRFIGEIISIHDSACTSEIVLLRFDDHSFALEVGILAPDADPDIQDLKDS
                     GNRAGAVVCWSIESNKLIVYAEFRTPQTSRSLEERHAAFLSKVKANGESTFPF"
     gene            complement(1690..>4481)
                     /locus_tag="PKK76_12360"
     CDS             complement(1690..>4481)
                     /locus_tag="PKK76_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNL69316.1"
                     /translation="LIYYPEGMEETAREASRLGENAAARYEKIFSHRLTKQIPVFLYA
                     SAQDFATTNILPFPLDESTGGFTDFYRRRVVLPFQGDPAVMRHVLDHELVHAFQFDIL
                     QGERLGSMPLWLMEGMAEYLSSGWDQTAETYVRDAVLNNRMPELVDLHNESVNDAYAY
                     YKGGQAVMMFIADTYGIDRIPFLLRAAHNTNRIDEAIRLTFKTSANEFNLAFARYLRA
                     RYALASRAYAETKDLRMREVTDRYESQTGFQIRPAVSPDGQSIAYLTVDGIFPAIVIR
                     PAPGPGITKKRQETKKIVLRALRSSDYEEVQLLTTKLSYSPDGKSLMVAGRRQGQQSI
                     LFFDVDSGDLIESESHALPLDSIAFPSMSPDGSKIVFSGTISGKTDLYILEKRSGMLR
                     RLTSDARYESSPVFSNAGDQIYFSASSESKIVSSQIYRFDLASGKVFQITNIPGQNGH
                     AISGQDGSILFLSTQDGVSNVFLLEHASTLTQPSSEVSAITASLTGIFDIAGAGEKGI
                     VFTERVEGASEVRLMPDVRTTQKLDENLITFHAKPAPMMELERQSAIRQIGGEYSPFL
                     AIEGMPFVFVTAAGGPDGKTSVAALGFMSLADESGDHRLSTLVSYNARPAELNLNLEY
                     AYLKYRTDFFVGAYRQSGIFAVFNFLDFSLNSLLYNPYFRVLDQNTTGAYGGFEYPLH
                     SFGSISLVMSAGREERIYDTVRPEEKQQPDLFQNHQSLGFAYQYNNIVYSMYGPLDGH
                     AFLFQYSVPVQFNGRDRELYVTTTEFRFHHLFDNYSSLALRAFGATASGRDASLYPFQ
                     IGGYYTIRGYRFLEFEGKHAFVMNLDYHFNFIEALRFGVPFSWSPGPVRGALFFDAGS
                     AFDRPQDFQGYSRKSGTTRDLHLSFGAGIHWANFLWFLIPGALMKIEWATPYDTKRSL
                     PLSKWKGAFSVGFNF"
BASE COUNT         1073 a         1051 c         1143 g         1214 t
ORIGIN      
        1 aacgagtgaa aagcctgtat atattcgtgt gttttttgtg tatatattcg tgtgtttttt
       61 ctgtgggaag tcgtggggat tttcctatgg tgaaatctac acgagaatct atgctttttc
      121 tatgattaaa cctatgaaat aagctatgtc aaaagctacg gaaaagtaag ggctttcaaa
      181 tattttgttt tttttgcaga ccgtgattat agaggtttta tgagtgttta cagagttttc
      241 attaatcaga atgacgatca actaggagca atttcatcag aggtttatgt agtgaaaaaa
      301 ggagatactt tgttttacgt atccaatcac gtggcgcgag gcggaggaat tcttggatac
      361 agatttggca aagaaactga tttagaggca gagcgtgcca ggtctctcta ccaggaggct
      421 atggttcttg gtatgctcaa cgccgccggg ttcttctttc tgatctactt tctcgcgcgc
      481 ggtggtggcg ccgcccttcc agtctttact ctgttgatga ttgtgtgcac cgtgagaagt
      541 gccgcatcca acagtgttct ggaagaatgg tttccggatc gccagctaac ggatcttcgc
      601 ctgcgcctgg aatacctcac ggcgcttgcc cttatgcctc cactttactt ctgggcgatt
      661 gctcttttgt ttcagggaga tcctgagacg agccttgcga cactgcgtcg cacgattacg
      721 cgcattctat ccgcagtcta ttcgatcggt gcgttttgcc tgagtctatt tttcatcttc
      781 gcacgcgacg cctccgttta cggaaagtat caacccgaat acgttagctg ctatcttctg
      841 ccggggcttg ctgtttgtct gttcttgctt ttcatacttg tgattgaaaa acgcagaggg
      901 gccggatgga tgcttcttgg tttcctgagt gttttgttct ctactctggc ggatgcagaa
      961 accactgtag ccggccaggc gggaacactc tacgtgcctc tgggacttct tggattcgca
     1021 gcctgctttt ctgtcacggt cgggattaga atcagaagcg acttcgagga actcgccctc
     1081 accagaaaga atctggcgct cagctttgag caagacagga gaagagagca gaaaagggcc
     1141 tccgagcgtg aacaaattgt actccaggca gtgtccgctc tttcgcgcac tgcctctgat
     1201 tttgaagaga tgctcagtca gggcaaagtg caaaaggacg cggccatccc tgctctttcc
     1261 agattgcatt cattgcgcgt ccggcttgag aagaggttga gtcgaactcc agtggctccg
     1321 gtcgcgctcg atgttcttcg ctacattcgc tttcatcatc cctctgttcg catcccatct
     1381 caggccagcc ttgtggtttt ttcaagcagg cgagagctcg accgtttcat cggggaaatc
     1441 ataagcattc atgacagtgc gtgtacgtct gaaattgtac tccttcgttt tgatgatcat
     1501 tctttcgcac tggaagttgg cattcttgct ccggatgcag atcccgatat acaggatctc
     1561 aaagattcgg gaaatcgcgc gggtgcggtt gtatgctggt cgatcgagtc aaacaaactg
     1621 attgtctacg cggagtttcg cacgccgcag acgagtcgga gcctggaaga acgtcatgcg
     1681 gcattcctct caaaagttaa agccaacgga gaaagcacct ttccattttg aaagaggcag
     1741 agatcttttt gtatcatagg gggtggccca ttcgattttc atgagcgcgc ccggtatgag
     1801 aaaccagagg aaattcgccc agtgaattcc agccccaaac gataaatgca agtcacgagt
     1861 ggttcctgat tttctggaat agccttgaaa gtcctgaggt ctgtcaaacg cagatcctgc
     1921 gtcgaagaaa agcgcgccac gcaccggccc cgggctccag ctgaacggaa cgccaaaacg
     1981 caatgcttct atgaaattga aatggtaatc gaggttcatt acgaatgcat gcttgccttc
     2041 aaattccaga aatctgtatc ctcgtatggt ataatatccg ccaatttgaa acggatagag
     2101 agacgcgtct cttccggatg cggtcgcacc aaacgcacgt aaggcgagag aagagtaatt
     2161 gtcaaacaga tgatgaaatc tgaattcggt tgtggtaacg taaagctctc tgtcccttcc
     2221 attgaattga acgggaaccg aatattgaaa tagaaacgcg tgtccgtcca ggggaccata
     2281 catagaataa acgatgttgt tgtactggta ggcaaatcca agagattgat gattctggaa
     2341 aagatctggc tgttgttttt cttccggtcg cactgtatcg tagattcttt cttcgcggcc
     2401 tgcactcatg acaagggaaa tggaaccaaa gctatgcagt ggatattcaa atccgccgta
     2461 ggcaccggtc gtattctgat cgagaacgcg aaagtacgga ttgtaaagca agctattgag
     2521 gctgaagtcc agaaagttga agactgcgaa tattccactc tgtctgtagg cgcccacgaa
     2581 gaagtccgtg cgatatttca aatacgcata ttccaggttc agattcaatt ctgcggggcg
     2641 agcattgtag cttacgagag tggacagtct atgatcccct gattcgtctg ccagagacat
     2701 gaatcccagg gctgccacag aagtctttcc atccggccct cctgctgcag tgacaaaaac
     2761 aaatggcatt ccttctatcg caaggaaagg tgaatattct cctccaattt gacgaatcgc
     2821 gctctgcctt tcgagctcca tcatgggcgc gggtttcgca tggaaagtaa tcaggttttc
     2881 gtcgagcttc tgtgtggtgc gtacatccgg catcagtcta acttcagatg ccccctctac
     2941 tctttctgtg aatacgattc ctttttcgcc agcacctgca atgtcgaaga tgccggtgag
     3001 gctggctgtg atggctgaca cttcagagga tggctgcgta agagtggaag catgttcgag
     3061 caagaagacg ttggatactc cgtcttgcgt gctcagaaac agaatgcttc cgtcctgtcc
     3121 gctgattgcg tggccatttt gtcctggtat gtttgtgatc tgaaaaactt tcccggacgc
     3181 aaggtcgaat ctgtaaatct gtgaggacac gattttgctt tcagaagaag cactgaagta
     3241 gatctgatct cctgcattcg aaaacaccgg gctgctttcg tagcgcgcat cagaggtcag
     3301 gcgcctgagc atgcctgacc ttttctctaa aatgtataga tctgtttttc cagaaatcgt
     3361 tccagaaaat acgatcttac tcccgtcagg cgacatagaa gggaaagcta ttgagtcgag
     3421 aggaagcgca tgtgattcgc tttcgatgag atcgcctgag tccacgtcga aaaacaaaat
     3481 cgattgctgg ccctgacgtc ttcccgctac catcaaagat tttccatcag gagaataaga
     3541 aagcttcgtt gtgagaagtt gcacttcttc gtaatcgctg gagcgtaagg cgcgaaggac
     3601 gatctttttt gtctcctgtc tcttttttgt aattcctggc cccggtgccg gcctgattac
     3661 aatggctgga aagattccgt ctacggttag ataggcgatg gactgtccgt ccggggaaac
     3721 agcagggcgg atctgaaagc ctgtctgtga ttcatatcga tctgtcacct ctcgcattct
     3781 aagatctttt gtttctgcgt aggcgcgcga agcaagcgcg taacgagcgc gaaggtaacg
     3841 cgcgaaagcc agattgaatt cgtttgcact ggttttgaac gttaggcgaa ttgcttcgtc
     3901 tatacgattt gtgttgtgcg cggcgcgaag aagaaatggg attcgatcga ttccatatgt
     3961 atctgcgatg aacatcatca cagcctgccc tcctttgtag tacgcgtatg cgtcgtttac
     4021 tgattcattg tgcaggtcta cgagctccgg catgcgattg tttagcacag catcgcgaac
     4081 gtaggtctcc gcagtctggt cccagccgct cgacaaatat tctgccattc cctccatgag
     4141 ccagagaggc atggagccca gacgttcacc ctggagaatg tcaaattgaa atgcgtgtac
     4201 cagttcatgg tccagaacat gtcgcatgac tgctggatct ccctgaaaag gaagaaccac
     4261 gcgacgtctg tagaaatctg tgaagcctcc ggtggattca tccagtggaa acggcagaat
     4321 gttggtggtg gcgaagtcct gtgcggaggc ataaagaaaa accgggatct gttttgtaag
     4381 cctatgcgaa aaaattttct catatctggc agctgcattt tcgcccagcc tggaagcctc
     4441 ccgcgcggtc tcctccatgc cctctggata gtagatcagg a
//
LOCUS       DAORFF010000309         4479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11261_length_4479_cov_3.686935, whole genome shotgun sequence.
ACCESSION   DAORFF010000309 DAORFF010000000
VERSION     DAORFF010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11261_length_4479_cov_3.686935"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..2263
                     /locus_tag="PKK76_12365"
     CDS             98..2263
                     /locus_tag="PKK76_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69317.1"
                     /translation="MRNLFNLAIAVLFTAAVGCTGTVGPASHGNGNMLTLFGVAALYH
                     GGSPIPTNEWASTGSHASNAITASEGGYVELGRNVKLAVPAGALDQDEYITVTAIDRP
                     AAADGVTPVLMGYKFEPEGLQFSKSALLTICYPSQELSSQGLSASTAQVYLQDEDGLI
                     GMGGSESGGCVTASVDHFSTYLAGAQALVAGSSAPSVSATANFLPGTPVAGIPLRIRT
                     IITSFQGTSTGSIAAAYVFWRTVGAPTYTQSVLTPDPTDAATPNRYTFLIPASAVTTT
                     GIQYYFRAIDNLGKITTTPVRTRTIARTATGITLNPAATLNIASGFSRDYTLSVTSDV
                     GGAQNISADTFNVGGGVGSAVKVSPSSIRFTAQGVGNGNLNVTAGSFSFSSPINVDPG
                     QLVNVTILDQSSVAITGTLNLSVNQVYAFDAIGRDAWGNVIPVLPSFGVTGPIGTIGT
                     SSGIFTASPTAGVSGTVDATLAGMTSSAQVTILSNAKALTAFSFPSLGATGVITGTNI
                     AITVPAGTNVSALTASFTTTGVSVKVGSTVQTSGSTPNNFAAPVTYTVVAEDGSFQDY
                     VVTLSNGPSVAANGMGPPNELQYCNLQFPNTMSVQTNQPTQNVYGRVYGQNVTEVAGP
                     NPIVLADFGYGPLGSDPINQPGLWTFVTAVYNVQIANDDEYMKSIIAPAPGSYAYVFR
                     FSMDGGAHYTYCDMDGAGYNPFPTNSFSPAQMGVMTVTP"
     gene            complement(2337..3491)
                     /locus_tag="PKK76_12370"
     CDS             complement(2337..3491)
                     /locus_tag="PKK76_12370"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNL69318.1"
                     /translation="MTTEKRPGLWQSLSGAHPGELPALDIVRAAAILMVFAYHAWTST
                     RTAVPAVAQVVDNLLLNGKSGVTLFFVLSGYLITSVLIREYATSANISLSQFYLKRSL
                     RILPAYYAYLIFFLILAVIGSHTFARGTENQFQRGWYFDFLFVSNIWQGFHVHTWSLA
                     TEEQFYLLFPVLAIALFRASKRIRIAIILALYLVPLAIRVVAFSKMEPALYGDWAYHR
                     PWTRFDDLLAGILVASAEISTMRAIYRRTILVVCSVAALSVLATSPSEFTWISPFLNN
                     GLNLSFAGIVLAAQASPLKKAPVFFTVTARLSYSFYLWHMLAGGIGAAVVFHSVKPGD
                     TVTWMKFARGILSAAFWSYVIALVSYRLIEMPFLRLRAPLLLRMQKKSST"
     gene            complement(3494..3907)
                     /locus_tag="PKK76_12375"
     CDS             complement(3494..3907)
                     /locus_tag="PKK76_12375"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNL69319.1"
                     /translation="MTFLKKYTSLFLKFGLVGALGTVINLAVFWLLVEVLRIEPNIGS
                     VAAFVTALTANYFLNQNWTFREEAGGELFTAGSYARYAGVNLVGLAVNLIVLNAAIWL
                     FHPQGLMLVQAAGIAAGTIFNFVLSKLFVFKAEQD"
     gene            4032..>4479
                     /locus_tag="PKK76_12380"
     CDS             4032..>4479
                     /locus_tag="PKK76_12380"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNL69320.1"
                     /translation="MRFTPYLLLLLILVLDQPVNLPWVHNAGRPEPTEHELLQTYVHN
                     ASAVLDADRQAGKKHVLLIGSSRSMMLGEIRHHDLNADPHLDDRSRATLGEFTFDPRL
                     AFPGANLLGELIVADELTRSGVKPDLIVLELGPEFLNEVLVESSYNQ"
BASE COUNT         1079 a         1152 c         1145 g         1103 t
ORIGIN      
        1 gtcgcataac gtttgttatt gtatgtgatt ttaaggaaac tatttgacct cacaggcccg
       61 ggcatacccc tctggtcgca gacgggggat ttgtgttatg agaaacttat tcaatttggc
      121 gattgcggtt ctatttacgg cggccgtggg ttgcacggga actgttgggc ccgcttcaca
      181 cggaaatgga aacatgctca ctctgtttgg cgtggctgct ctctatcacg gaggttctcc
      241 gatcccgaca aatgaatggg ccagcacagg gtcgcatgca tccaatgcaa tcactgcatc
      301 ggaaggcgga tatgtggaac tgggtcgcaa cgtgaaactt gcagtacctg caggtgctct
      361 tgaccaggat gaatacatta cggtcaccgc gatcgatagg cccgcagcag cggatggcgt
      421 tactccggtt ctcatgggtt ataaattcga accagaagga cttcagtttt caaagtctgc
      481 acttctcacc atatgctatc catctcaaga gctcagttcg caaggcctct ccgcttctac
      541 agcgcaagtc tacctccagg atgaggatgg tctcattggt atgggaggtt ccgaatccgg
      601 cggttgcgtc accgcatctg tagatcattt ctcgacttat cttgctggag cgcaggcgct
      661 cgtggccggc agctcagcac cttccgtcag tgccaccgca aactttcttc caggcacacc
      721 tgtggcagga attccgcttc gcattcgcac catcatcacc tcgtttcaag gaaccagtac
      781 cggatccatc gcggcagcgt acgtattctg gagaacagtg ggtgcgccta cgtacactca
      841 gtctgtcctc acgcctgatc caacagacgc ggctacaccg aatcgctata cattcctgat
      901 cccggcgagt gcagtaacca caaccggaat ccagtactac ttccgtgcaa tagacaatct
      961 tggaaagatc accacgacgc ccgtgcgtac tcgaaccatt gctcgcacgg caactggcat
     1021 cactctcaat ccggcggcca ctctcaatat cgcttctggc ttttcacgcg actacactct
     1081 gagtgtaacg agcgatgtag gaggcgcgca gaatatctcc gcagatacgt tcaatgtagg
     1141 cggtggcgtt ggcagcgcgg ttaaggtttc cccttcgagt attcgcttca cggcgcaagg
     1201 tgtgggcaac ggaaatctga atgtcactgc tggctccttt agtttttcct ctcctatcaa
     1261 tgtggatccg ggtcagcttg tgaacgttac gatattggat cagtccagtg ttgcgatcac
     1321 cgggactctg aatctgtcag tgaaccaggt atatgcattt gatgcgatcg gtcgggatgc
     1381 ctggggcaat gtgattcccg tattgccttc ttttggagtc actggcccca ttggaaccat
     1441 cgggacatct tctggcattt tcactgcatc gcctactgca ggagtgagcg gcactgtaga
     1501 tgcaacgctg gcaggtatga cgtcctctgc gcaggtgacc atcctctcga atgcgaaagc
     1561 cttgactgct tttagttttc cctctctggg agctacagga gtgattacag gcacgaacat
     1621 tgcgattact gtgcctgcgg gaacgaatgt ttctgctctc actgcaagtt tcacaacgac
     1681 aggagtctcc gtaaaagttg gctccaccgt tcagacgagc ggatcgacgc cgaacaattt
     1741 cgcggctccc gttacgtata ctgtggttgc agaagatgga tccttccagg attacgtagt
     1801 cactctgagc aatggaccga gcgttgctgc aaacggaatg gggccgccta acgagttgca
     1861 atactgcaat ctacaattcc cgaatactat gtccgtccaa accaatcagc caacgcaaaa
     1921 tgtctatggc cgagtttacg gacagaatgt cacagaggtt gcaggtccca atccaatcgt
     1981 gctggctgat tttggctacg gccctctggg ctccgatccc atcaatcaac ccggtttgtg
     2041 gactttcgtc actgcggttt acaatgttca aatagccaac gatgacgaat acatgaagtc
     2101 gatcatcgcg ccggctcctg gttcttacgc gtacgtgttc cgtttctcca tggatggagg
     2161 agcacactac acctattgcg atatggacgg agccgggtac aatccgtttc caacgaacag
     2221 tttctctccg gcgcaaatgg gcgtgatgac tgtcactccc tgatccgcga gactgattca
     2281 tgaacgattt cggtggcgat cgtggccatc gaaatcggct tgatgtctgg ttgaattcaa
     2341 gtgctcgatt tcttctgcat tcgtagcagg agcggtgctc tcaagcgaag aaaaggcatt
     2401 tctatcaagc gataagaaac cagagcaatg acataactcc agaatgcggc agaaagaatt
     2461 cctcttgcga atttcatcca cgtaacagtg tctcctggct tgacggagtg aaacaccact
     2521 gccgccccta tgccgcctgc gagcatatgc cataaataga aggaataact caaccgagct
     2581 gtgacggtga agaacaccgg cgcttttttt aagggagaag cctgagccgc cagcacaatc
     2641 ccggcgaagc tcaaatttag accgttgttc aggaaaggac tgatccaggt aaattcgctg
     2701 ggcgatgtgg cgagtacaga taaggccgcc acggaacata caaccagaat cgtgcgcctg
     2761 tatatcgcgc gcatagtaga gatctctgca gacgcgacca gaattcctgc cagtagatca
     2821 tcaaatctgg tccacgggcg atgataagcc cagtctccat acaatgcagg ttccattttt
     2881 gaaaatgcaa cgacgcgaat tgcaagtgga acaagataca atgccagaat gatcgcgatt
     2941 cgtattctct tgctggctcg aaacaacgcg atcgcaagga cggggaacaa caaatagaac
     3001 tgttcttcgg ttgccagaga ccacgtgtga acgtgaaacc cttgccagat attcgaaacg
     3061 aaaagaaaat cgaaatacca acctctctga aattgattct ctgttcctct tgcaaaggtg
     3121 tgcgagccga tgactgcaag aatcaagaag aaaataagat aagcatagta cgcgggcagg
     3181 atgcgaagac tgcgtttgag gtagaactgc gaaagggaaa tgttcgctga tgttgcgtat
     3241 tcgcgaatta aaacggacgt gatcagatag ccactcagaa caaagaatag tgtcacaccg
     3301 cttttgccat tcaacagcaa gttgtctact acctgggcca ctgcgggtac cgcggttctt
     3361 gtactggtcc atgcgtgata ggcgaatacc atgagtatgg cggccgcgcg aacaatgtcg
     3421 agcgctggca attcgccggg atgggcaccg gacaaagatt gccacagacc aggtcttttc
     3481 tccgtcgtca ttttcaatct tgttctgcct tgaatacaaa cagtttcgaa agtacaaaat
     3541 tgaagatcgt tccggcagca atccctgcag cctgaaccag catcaatccc tgaggatgaa
     3601 acaaccagat ggcggcgttc agtacgatta gattcacagc gagtccaact agatttaccc
     3661 ccgcatatcg cgcatacgac ccggcggtga atagctcgcc tccggcttcc tctctgaagg
     3721 tccagttctg attcaaaaag taatttgctg tgagtgcggt cacgaacgct gccacactcc
     3781 ctatgtttgg ttctattcgg agtacttcca cgagcaacca gaacactgcc aggttgatga
     3841 ccgtacccag cgcgcccacg aggccgaatt tcaaaaatag agaagtgtat ttttttaaga
     3901 acgtcatact gtggatcgtt ttatcgtcga tccaggttcg aagatcgtat gtgctcggat
     3961 tatttctcct ctttttttgt gtttcgattt ttgattgcgc ggtcggtcag tattggcttg
     4021 atcagcagaa gatgaggttt accccttact tgcttctgct cttgattctg gttctggatc
     4081 aacccgtgaa tttgccctgg gtccacaatg cgggcaggcc agaacccacg gagcatgagc
     4141 tgctgcagac ttacgtccac aatgccagcg cggttcttga tgcggaccgg caggcgggaa
     4201 aaaagcatgt actcttgatc gggtcttcgc gtagcatgat gctcggggag attcgccacc
     4261 atgacttgaa tgcggatcca catctcgatg atcgtagccg tgcgacgctc ggcgagttta
     4321 cttttgatcc aaggctggct ttcccgggtg cgaacctgct tggagagttg attgttgcgg
     4381 atgagctaac gcgttcaggt gtgaagccgg atctcattgt tctggagctg ggccctgagt
     4441 tcctcaacga ggttctggtt gaaagttcct acaatcaga
//
LOCUS       DAORFF010000310         4472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11290_length_4472_cov_2.896310, whole genome shotgun sequence.
ACCESSION   DAORFF010000310 DAORFF010000000
VERSION     DAORFF010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11290_length_4472_cov_2.896310"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PKK76_12385"
     CDS             complement(<1..741)
                     /locus_tag="PKK76_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69321.1"
                     /translation="MAFLRDWPVKLGALVVAGILALYVEFAQNVTRVQHVRVTVPPIP
                     PGLVFASRVPSFMDVELYGPSELMDVAASDFKMQLINRRPVRGENIFRVKMSPELPSG
                     LEASYPKELKVMLDEVLYRELPVEAAYDHSMPLGGIRIRPRTVVVVGPYQKMAEMDRV
                     MTLPVTLSGSGSRSIRAVLADLPDFMSLAEGQPNEVQLDVWDKQEKDPTYETVEGIPV
                     RCSNNVVGLRLRQPPSVRIRVPIQGARAD"
     gene            complement(771..1577)
                     /gene="cdaA"
                     /locus_tag="PKK76_12390"
     CDS             complement(771..1577)
                     /gene="cdaA"
                     /locus_tag="PKK76_12390"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNL69322.1"
                     /translation="MLENLWNSVRPFVRFIDILIVAFLLYRAYILLSRTRAMQLLLGF
                     GLILLLDVIARRFSMDTLSWLITNVSQYLVFGLIVLLQPELRRLFAEIGKMPLFQWVN
                     PPLSVPLDEIIAAASSMARSRTGSIIVLMKEIRPHGILENSVRVEGNISRELLETIFH
                     KDTPLHDGAVIIEGAKILAASCYLPLSSSRVLKKTLGARHRAALGMSEESDAVILVTS
                     EESGKISVMQNGEVLTPVRAGELKSLLSELLGKTPRSIAEIAEEKKAQEA"
     gene            complement(1570..2313)
                     /gene="dapB"
                     /locus_tag="PKK76_12395"
     CDS             complement(1570..2313)
                     /gene="dapB"
                     /locus_tag="PKK76_12395"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNL69323.1"
                     /translation="MIRFVMSGARGRMGQMILNTATLPDFKDRITASGQIDVGDDPVS
                     QIQGASVMIDFSSPANTLAMAKLCMAHNVGFIVGTTGLTSAQKAEIESASSKIPVLIS
                     PNMSLGVNLLFHLTGIAVSAVPQADVEIVELHHNQKVDAPSGTAARMKDIVKEKRNLA
                     EKDVVHGREGLVGKRKQEEIGMHAVRGGDTPGDHTVYLFLDGERLEITHRALSRIVFA
                     RGALQAALFLSGKPAGIYTMNDVLGIANA"
     gene            complement(2310..3194)
                     /gene="dapA"
                     /locus_tag="PKK76_12400"
     CDS             complement(2310..3194)
                     /gene="dapA"
                     /locus_tag="PKK76_12400"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNL69324.1"
                     /translation="MEFRGLYTAVVTPFTSGGSQIDYDAYAKLLERQIAGGVAGVVPC
                     GTTGESPTLTHEEHSELIRRTVSLVKKRVQVIAGTGSNSTAEAIELTKAACSDGVDAV
                     MIVNPYYNKPTQEGLFRHFEAVAGVSSVPVMVYNIRGRTAVNIEPETFTRLKSVARIA
                     CVKEASGDLGQMARIHQLAGDRIALLSGDDNLTPAVMAIGGLGVVSVASNIFPKRLSA
                     MVKHYLAGDYKSGNAIFYDLLEFMQALFWETNPIPVKAGLAALGLCKPDIRLPMTPMS
                     EGKRREFEILLNALGEDR"
     gene            3252..4325
                     /locus_tag="PKK76_12405"
     CDS             3252..4325
                     /locus_tag="PKK76_12405"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69325.1"
                     /translation="MSGTATPVIAFLCFVSSFVAAGLLTRIQLLPDRPNPRSMHTRVT
                     PRSGGLSLQVPFLMAIAYFAWRDGWSAHVLAVLVGSTAFSLLGLVDDAIQLSAGSRLV
                     FQIAAASLLCYYAAPDSVQILDLLVVSGPPALAFQILWTVVCINFFNFMDGMDGLAGF
                     QAFWFTFVGGWFIMLTAGDSPVIGRGLLCLSASLLAFLLWNLRPSTLFMGDSGSYFLG
                     FLIGFLPLVWGHPEPRGPLLKWQPDEIAGGLDFTLGLLLFAPFLLDATLTIFRRLSDR
                     HNIFSAHRTHLYQLLRQNGWSVSRVLVLYQIANLVCAGIAVWRERANVTADYFAAGLF
                     LFAVVVTFIFSAATRYLLSKKAA"
     gene            complement(4276..>4472)
                     /locus_tag="PKK76_12410"
     CDS             complement(4276..>4472)
                     /locus_tag="PKK76_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69326.1"
                     /translation="TLLFWFLQFALPRMGWHLDPILSRVPEKVHVPEIQTPDVKMPDL
                     KIPEVKLPSLKGDSGSQQKK"
BASE COUNT         1034 a         1217 c         1110 g         1111 t
ORIGIN      
        1 atctgctcgc gctccttgaa tcggcactcg aatgcgcaca gaagggggct gtctcaaacg
       61 cagacccaca acgttattcg aacagcgcac aggaattcct tccaccgttt cgtatgtggg
      121 atctttctcc tgcttgtccc acacatcgag ttgcacttca ttgggctgcc cttccgccag
      181 actcataaaa tcaggcagat ccgcgagaac tgcgcgaata gaacgagaac cggaaccgct
      241 gagagtcaca ggcaaagtca taacgcggtc catctcggcc atcttctgat acgggccaac
      301 aacaactaca gtgcgtggtc gaattcgaat cccgccaaga ggcatggagt ggtcgtaggc
      361 tgcttctacg ggtagctcac gatacaacac ttcatcgagc attactttca attctttagg
      421 atacgaagcc tcgagtcctg atggtagctc gggggacatc ttcacgcgaa agatgttttc
      481 ccctctcaca ggcctgcgat tgatgagttg cattttgaaa tcagacgccg ccacatccat
      541 caactcggac ggaccataca gttcaacgtc catgaaagaa ggaaccctgg aagcaaatac
      601 cagcccgggc ggaatgggtg gaacggtaac gcgcacatgc tgcacccgag tgacgttctg
      661 ggcaaactca acataaagag ccagaattcc tgcaaccaca agtgcgccaa gcttcactgg
      721 ccagtcgcga agaaaggcca tggccctctt ccatagttga cgaaatccga tcaagcttcc
      781 tgcgccttct tctcttccgc aatttcagcg atggatcgag gagttttccc aagaagttca
      841 gacagaagag atttgagctc acccgcacgc acaggtgtga ggacttctcc attttgcatg
      901 acgcttattt ttcctgattc ctcagaagta accaggatga ccgcatccga ttcttccgac
      961 atgcccaggg ctgcgcgatg gcgcgcgcca agcgttttct tcaaaacgcg cgagcttgat
     1021 agaggaaggt aacaggacgc agccagaatc ttcgcacctt ctatgataac ggccccatca
     1081 tgaagcggag tatccttgtg aaagatggtt tcaaggagtt cgcgtgaaat gtttccttct
     1141 actctgacag agttttcaag aatgccatga ggacgaatct ctttcataag gacgatgatc
     1201 gagcccgtcc tgcttcgagc catactcgat gcagccgcaa tgatttcatc gagtggaacg
     1261 ctgagtggtg gatttaccca ctggaacagc ggcattttgc cgatttcagc aaacaacctg
     1321 cgaagctccg gctgaaggag cacaatcaaa ccaaatacga gatactgcga aacgtttgtg
     1381 atcaaccagg acagagtgtc catggagaat ctacgcgcga tgacatccag aagcaggatg
     1441 agtccaaagc caagcagaag ctgcatggcg cgcgtgcgtg acagaaggat gtaagcgcga
     1501 tataaaagga atgcaactat cagtatgtct ataaaacgaa caaacggacg aaccgaattc
     1561 cagagattct caagcattcg caatccccag aacatcgttc attgtgtaga ttcctgccgg
     1621 cttcccggac aggaagagcg ccgcctggag tgcgccacgc gcaaatacaa tgcgtgataa
     1681 tgcgcgatgg gtgatttcca gcctttctcc gtctagaaat agatacacag tatgatcgcc
     1741 gggtgtatca ccgccgcgca ctgcgtgcat accaatttct tcctgcttgc gcttgcctac
     1801 gagcccttct cttccgtgta ccacgtcctt ttctgcgaga tttctcttct ctttgacaat
     1861 gtccttcatg cgcgccgctg tgccggaagg cgcgtccact ttttgattgt gatggagctc
     1921 tacaatttcc acgtctgctt gcggaacagc tgagactgcg attcctgtaa gatgaaaaag
     1981 caaattcact cccagtgaca tgtttggact gatgagcact ggaattttag aagatgcaga
     2041 ctcaatttct gccttctgtg cggaggtgag cccggtggtg cccacgataa aaccaacgtt
     2101 atgcgccatg cataactttg ccatggcaag ggtatttgcg ggacttgaaa aatcaatcat
     2161 gacgcttgca ccctggatct gagaaacagg gtcatctcca acgtcaattt gtccggatgc
     2221 cgttatgcga tctttgaaat caggcaacgt ggccgtattc aagatcatct ggcccattct
     2281 accacgggcg cccgacatca caaaacgaat catctatctt ctcccagagc attcagcagt
     2341 atttcgaatt cgcgacgttt gccttcgctc atcggagtca tggggaggcg gatgtcaggc
     2401 ttgcacagac ccagggccgc cagacctgct tttactggaa ttggattggt ttcccagaaa
     2461 agagcctgca tgaactcgag aaggtcgtag aagatcgcat taccagactt ataatcgcct
     2521 gcaaggtaat gcttgaccat cgcagagagt ctctttggaa aaatattgga tgcgacggat
     2581 acaactccaa ggccgcctat cgccatcacc gcaggcgtta gattgtcatc ccccgaaaga
     2641 agtgcgattc tatctcctgc cagctgatgg atgcgagcca tctgtccaag atctccgctt
     2701 gcttctttta cgcaggcgat gcgcgcaacg ctcttgagtc gtgtgaatgt ctctggctca
     2761 atattgactg ccgttctgcc gcgaatgttg tagaccatca ccgggacact cgatacgcct
     2821 gccactgctt caaaatggcg aaagagacct tcctgagttg gcttgttgta gtacggattg
     2881 acaatcatca ccgcatctac tccatccgag caggcagctt tcgttagctc gatggcctct
     2941 gcagtggaat ttgacccggt gcctgcgatg acctgcaccc tttttttcac aagagagact
     3001 gtgcggcgga tgagttcact gtgttcctca tgggtgagcg taggagattc gcccgtggtc
     3061 ccgcagggga ccactcctgc cacgcctcca gctatctgac gctccagaag cttcgcgtac
     3121 gcatcatagt cgatctgact gcccccggat gtgaaaggcg tgacgactgc cgtatagaga
     3181 cccctgaatt ccatcttcga cctggcctaa atattatttc aagaaaagcg gcttctcttt
     3241 tttgtgcaaa catgtctgga acggccactc cggtcatcgc ttttctgtgt tttgtatcca
     3301 gctttgttgc ggccggactg ctcacacgca ttcaacttct gccggatcgc cccaatccgc
     3361 gctccatgca cacgcgggtc acaccgagaa gtggcgggct cagcttgcag gtgccttttc
     3421 tcatggcgat cgcatacttt gcgtggcgag acggatggag cgcgcacgtt ctggcggtgc
     3481 tcgtgggatc caccgcattc tctcttcttg gcctggtcga tgacgcgatc cagctttccg
     3541 cgggaagcag actcgtattt caaattgcag cagcaagcct tctgtgctac tacgccgccc
     3601 cggattctgt tcagattctg gatttgctcg tcgtctccgg acctccagcg ctcgcctttc
     3661 aaatcctgtg gactgtagtc tgtatcaact tcttcaattt tatggacggc atggatggcc
     3721 tggctggatt tcaagccttc tggtttacat tcgttggggg atggtttatc atgctcaccg
     3781 ccggtgacag cccggtcatc ggccgcggac ttctttgctt gtctgcaagc cttcttgcgt
     3841 ttctactctg gaaccttcga ccatcgactc tgttcatggg ggactcaggc agttactttc
     3901 taggatttct cattggcttt ctcccgctcg tctggggtca tccggaacct cgcggtccgc
     3961 ttctgaaatg gcagccggat gaaattgccg gcggtcttga ttttactctc gggctcttgc
     4021 tctttgcgcc attccttctg gatgcaactc tcacgatctt caggcgattg tctgacagac
     4081 acaatatctt ctccgcgcac agaactcatc tctaccagct cctgcggcaa aatggatggt
     4141 ccgtaagcag agtactggta ctctaccaga tcgcaaacct cgtgtgcgcg ggcattgcag
     4201 tctggaggga aagagcaaac gtcaccgcag attactttgc ggccgggctg ttcttgttcg
     4261 cagtcgttgt gacttttatt ttttctgctg cgacccgcta tctcctttca aagaaggcag
     4321 cttaacttcc ggaattttca gatccggcat ctttacatcc ggagtttgaa tctcagggac
     4381 atgcactttt tctggaactc gcgaaaggat tggatcgaga tgccaaccca tgcgaggcaa
     4441 agcgaattga agaaaccaga acagtagagt ga
//
LOCUS       DAORFF010000311         4454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11376_length_4454_cov_4.277108, whole genome shotgun sequence.
ACCESSION   DAORFF010000311 DAORFF010000000
VERSION     DAORFF010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11376_length_4454_cov_4.277108"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKK76_12415"
     CDS             <1..248
                     /locus_tag="PKK76_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69327.1"
                     /translation="IYLIWFRRPFDRGNLSLTFIWQFCNLFAFWAAVFLAPVYGGAVI
                     DGKYHTQIFGINENILAFGFFSLLWLTGLFIFRARSR"
     gene            251..2005
                     /locus_tag="PKK76_12420"
     CDS             251..2005
                     /locus_tag="PKK76_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNL69328.1"
                     /translation="MLKTDVLIVGGGPAGLVSSLCLATLGIRSIVVEKRPHHATHPKA
                     HEISARTLEILESIGIPIRELAKEASPHEDASRIVFCRTMAEEIGRIDLNQPDIQKKY
                     LNHVSVSRPYLNLSQTELEKILRKHVAENQMVQLLTGLEWVSLTQSPHNVISALRTTD
                     GKMNQIQSKYLLGCEGAGGAVRRSLGIKMEGPDQIQDFANAFFTNDLSKIAPRAKLFF
                     VFRPEAAGTLIAHHSRKRWVYHVPLLPHQKAADCDADFFRKRIRLALDIPDFEPNIES
                     ISSWRMTAQVAARFRKDRVFLVGDSAHRFPPTGGLGLNSGVADAHNISWKIAHVLKGE
                     NDRILETYEEERKPVVAFNCEESRRNYFNLLSIPAALGLHKFALDWGARILYSFPLRW
                     LGKSREPILACAMRFADRRLKIALTDKNSRRVTGARLEIHNQISHFDRIGLDLGYRYS
                     PDALLPDQIRFPDSTSTEYRPSFAPGARLPHFEVPGRGHASIHTRLRYDRWSLLCSGE
                     NASWSRAIRNAGLHRKIQVIPILNEVRMGDQTLSFAALAKMEEGGGLLIRPDGHVAAR
                     MRSRDDLYQTLNRFFSVP"
     gene            2046..3104
                     /locus_tag="PKK76_12425"
     CDS             2046..3104
                     /locus_tag="PKK76_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL69329.1"
                     /translation="MRYSFFLLFLSLAVAVHAAPSITPESMALYHAGHSAAVSGDRAA
                     ARGKFDAALARDPKNFLAMFDIGGLAEDAGRKEEAIEAYEKTTVIAPDFAEAQVELGT
                     LYLNVQRNPDKAIARFRIALLTKVPYADSRFALSHTRGQALRNLSVSYAVKRQFGLAQ
                     GIARSFLSDSGVNHEGDAAMNTLLQRSSGSMTAVTTRKFAEDLEPISKQLHGGKVAEA
                     LSAYLAFEKSHPLASLPPMDAWDLYEGIGLAYAMTNRNAEALVAFEKAVEAGMPLPYG
                     KLQEAQFNTACALALLGRAEEAIARLEEVLWEDQITTVDPERTRKNGYAPLIKKDETL
                     ASIRQHPGYLKLFETYGP"
     gene            3147..3569
                     /locus_tag="PKK76_12430"
     CDS             3147..3569
                     /locus_tag="PKK76_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69330.1"
                     /translation="MKRLFLALIVLSPFAIHASAVRECKVNATITRIMRDGKSPSFEI
                     HATRAAFERGHSMGEDCSELLMFEGNHVVELKDLSSPFKLQVGAPIILLFVEVRGVTK
                     DGPQMSRHWEVYPENPKSSCDENAYMVTRPKVRILERF"
     gene            complement(3532..>4454)
                     /locus_tag="PKK76_12435"
     CDS             complement(3532..>4454)
                     /locus_tag="PKK76_12435"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNL69331.1"
                     /translation="VVILNPRSKNGASLKAFNKLKPDLHKLLGPFEVYQTKAPRDATE
                     QVRSILKQGSFDQILIAGGDGSINEAVNGYFHEGKLLRADVPLGVINLGTGGDFYKTL
                     LRDSSNYQEALSQNRLRMVDCGLTTLEKGLDPQYFINITSIGLGGDVNRQMKASSFQS
                     GMPAYFYHSLTSLLKYSPPNCKFIMKDKEGKTTEIETGLVNFFVCNAEFNGGGMRWAP
                     ESDIEDGVFDLVLVSNVPKHKLITESHKIYSGKIREMTGVQEFHASEVIALPERTVSQ
                     EIDGETRDMDHLRTHEFHFKIVPRSLPLVL"
BASE COUNT         1057 a         1110 c         1165 g         1122 t
ORIGIN      
        1 aaatctactt gatctggttc aggcgacctt ttgatcgcgg caatctttcc ctcacattta
       61 tctggcaatt ctgcaatttg tttgctttct gggccgccgt cttcctggca ccggtttatg
      121 ggggggccgt catagacgga aagtatcaca ctcagatttt cggaatcaac gaaaacatcc
      181 tcgcatttgg tttcttctcc ttgctctggc ttacagggct gttcattttt cgcgcgcgca
      241 gtcgctaagg atgttgaaga cagacgtact catcgtaggt ggagggccgg ccgggctggt
      301 cagctctctg tgccttgcaa cgcttggaat caggagcatc gtggtggaga aaagacccca
      361 ccacgcaact cacccgaagg cgcatgaaat cagtgcgcgt actcttgaga ttctagaatc
      421 cattggaatt ccgatcagag agcttgcaaa ggaagcttcg ccgcacgaag acgcatctcg
      481 cattgtattt tgtagaacaa tggcggaaga aattggccgg atcgatctga accaaccgga
      541 catccagaag aagtatctga accatgtgag cgtttcgcgt ccctatttga atttgtctca
      601 gactgaactg gaaaaaatcc ttcgaaagca tgtggcggag aatcaaatgg tgcaacttct
      661 taccggtctc gaatgggtca gtctgactca atcgccgcat aacgttatat ccgctcttcg
      721 cacgacagat ggcaaaatga atcaaattca aagcaaatac ttactgggct gtgaaggcgc
      781 gggtggcgcg gtgagaagaa gtctgggaat taaaatggaa gggcccgatc aaatccagga
      841 ttttgcaaat gcgtttttta ccaatgattt atcaaagatc gcaccgcgag caaaactgtt
      901 cttcgtattc agacccgaag ccgccggaac cctcattgct catcattcgc gaaagagatg
      961 ggtgtatcac gttcctcttc tgccacacca gaaagccgcg gattgcgacg cagacttctt
     1021 tcgcaaacga attcgactcg ctctggacat tcctgatttc gaaccgaaca tagaatccat
     1081 cagttcctgg aggatgaccg cacaggtcgc cgcgcgtttt agaaaggaca gagtgtttct
     1141 tgtgggggac agcgcgcatc gatttccgcc tacgggtggt ctgggattga attccggagt
     1201 ggcggatgct cacaatattt catggaagat cgctcatgtg ctcaagggag aaaacgatcg
     1261 aattctagaa acgtacgaag aagagcgtaa gccagtggta gcattcaact gcgaagaaag
     1321 tcgtcgcaac tatttcaatt tgctctctat accggctgcg ctgggcctcc acaagtttgc
     1381 cctcgattgg ggcgcacgca ttttgtacag ctttccactc cggtggctcg gaaaaagccg
     1441 ggagcctatt cttgcctgcg cgatgcggtt cgctgatcgg cgtctgaaaa tcgcgcttac
     1501 tgacaagaac agcaggcgcg tgactggagc tcgcctcgaa atccacaacc agatttccca
     1561 ctttgatcgc attggactgg atctgggtta taggtacagc cccgatgctc tgttgccgga
     1621 tcaaattcga tttccggatt ctacttcaac tgagtacaga ccttccttcg cacccggagc
     1681 ccgtctgcct cactttgaag ttccgggtcg gggccatgcg tcgattcata caaggttaag
     1741 gtatgatcgc tggagcctgt tgtgttctgg cgaaaatgct tcgtggtcgc gtgcgattcg
     1801 gaacgcggga ctccatcgaa aaattcaggt cattcctatt ctgaatgaag tgcgcatggg
     1861 cgatcagact ctttctttcg cggctctcgc aaagatggag gagggcggag gcttgctcat
     1921 caggccagac ggtcacgtag cggcccgtat gcgatcgaga gacgatttgt accagacact
     1981 gaacagattc ttcagtgtgc cataattttg gctatgaatc cgggaaggtc gtttcatagt
     2041 ttagcatgcg ctacagtttc tttcttttat tcttgtcgct tgcagttgcg gtgcacgcgg
     2101 cacccagcat cacccctgaa tccatggcat tgtaccacgc cggtcacagt gctgccgttt
     2161 ctggagatcg tgccgcggct cgcggcaaat tcgatgctgc tctggcgcgg gatcctaaga
     2221 attttctggc tatgtttgat attggagggc ttgcagaaga cgcgggccgc aaagaagagg
     2281 ccatagaagc ctacgaaaaa acaaccgtca tcgcgccgga tttcgcagaa gctcaggtgg
     2341 agctgggaac tctttatctc aatgttcaga gaaacccaga caaagcgatc gcaaggtttc
     2401 gcatcgccct tttgaccaaa gttccttacg cggattccag attcgctctt tcgcatacgc
     2461 gtggtcaggc gctacgcaac ttatccgtca gctatgccgt taagcggcaa tttggactgg
     2521 cgcaggggat tgcccggagc ttcctttctg attccggagt caatcacgag ggggatgccg
     2581 ctatgaatac tcttctccag aggtcctcag gatccatgac tgcggtcacc acgaggaaat
     2641 tcgcggaaga ccttgagcca atatcaaagc agctacacgg aggaaaggtc gcagaggcgc
     2701 tcagtgcata tcttgctttt gaaaaatcgc acccgctagc cagcttgccg cccatggatg
     2761 cctgggatct ttacgaaggc attggtcttg cttatgcgat gaccaatcgc aatgcggaag
     2821 ccctggtagc ttttgaaaaa gcggtagaag cgggcatgcc gctcccatat ggaaagctac
     2881 aggaagcgca gttcaataca gcttgcgctc ttgcgcttct gggccgggca gaggaagcta
     2941 tcgcacgtct ggaagaagtt ctctgggaag atcaaattac cactgtggat ccggagcgca
     3001 cgcgtaagaa tggctatgcg cccctcatca aaaaagacga aactctggct tccattcggc
     3061 agcatccggg ttatttgaag ttgttcgaaa catacggacc ctgaaaaaac ggaaaacaaa
     3121 tccattcatc gttgcgtcgg atgagcatga aaaggttgtt tctagccctc atcgtgctca
     3181 gtccatttgc gattcatgcc tctgccgtgc gcgaatgcaa ggtaaatgcg acgattacca
     3241 ggatcatgcg ggatggaaaa tcgccttcat ttgagatcca tgcgactcgt gccgcgttcg
     3301 aacgcggaca tagcatgggg gaagattgca gtgaactttt gatgttcgaa ggcaatcacg
     3361 tagtcgaact caaagacctt tcatctcctt tcaagctcca ggttggcgct ccgatcattc
     3421 tactgtttgt tgaagttcga ggtgttacga aagacggccc acagatgagc aggcattggg
     3481 aagtctatcc cgaaaatcct aaatcatctt gcgatgagaa cgcgtatatg gttacaagac
     3541 caaaggtaag gatcttggaa cgattttgaa atggaattcg tgcgttcgca aatgatccat
     3601 gtcacgcgtt tccccgtcta tctcttgcga tacagttctc tccggtagag cgatcacttc
     3661 cgatgcgtgg aactcctgca cgcctgtcat ttcgcgaatc ttgccgctat agattttgtg
     3721 ggattctgtg atgagtttat gcttgggcac gttggataca agaaccaggt caaacactcc
     3781 gtcttctatg tcagactctg gagcccatct catgccaccg ccgttgaact ctgcgttgca
     3841 cacaaagaaa ttcacaagcc ctgtttcaat ctcagtagtc ttcccttctt tatccttcat
     3901 gataaacttg cagttaggcg gtgagtattt caacagactt gtgagcgaat gatagaagta
     3961 cgcaggcatc ccactctgaa atgatgacgc tttcatctgg cgattcacgt ctccgcccag
     4021 accaatggat gtgatgttga tgaagtattg tggatcgagc cctttctcga gagttgtaag
     4081 gccacagtct accatgcgaa ggcgattttg agaaagcgcc tcctggtagt tggaggaatc
     4141 gcgcagcaac gttttgtaaa agtccccacc cgtgcccagg ttgatgacgc ccagtggaac
     4201 gtctgcgcga aggagctttc cttcgtgaaa gtagccgttg actgcttcgt tgatggatcc
     4261 gtctccgccc gcgatgagga tctgatcgaa actaccctgt ttgagaatgg agcgcacttg
     4321 ctccgtggca tctctgggtg ccttcgtctg gtaaacttcg aatggcccga gcagtttgtg
     4381 gagatccggc ttgagcttgt tgaatgcctt gagggaggct ccattcttgc ttctcgggtt
     4441 gaggatgacg actc
//
LOCUS       DAORFF010000312         4453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11380_length_4453_cov_3.122556, whole genome shotgun sequence.
ACCESSION   DAORFF010000312 DAORFF010000000
VERSION     DAORFF010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11380_length_4453_cov_3.122556"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /gene="radA"
                     /locus_tag="PKK76_12440"
     CDS             complement(<1..1353)
                     /gene="radA"
                     /locus_tag="PKK76_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNL69332.1"
                     /translation="MAEKKPRKSYECTSCGAKSSRWEGKCGTCGEWNTMVESELQPGL
                     VPGKRAAQGHASFESLSEANADIARITTGIQDLDNVLGGGLVPGALVLLGGEPGVGKS
                     TLLLEIARRFEGKFLYFSGEESPGQIRIRATRLGVMSSNVQVSRETDLETIAQEIEKH
                     KPDIAVIDSIQTVQSAGSSPGSPAILREAAFRLMEAAKSCSVPILITGHITKDGAIAG
                     PRLLEHMVDTVLLFESDRLKHHRILRAVKNRFGPIGEVALFEMGNAGLHSVLSLAPHR
                     EDNPAPGRVYSVLQEGSRSICVEVQALVSRCYAGPPRRMAEGLDNRRLILLGAVLEKY
                     LKLRLSEFDLFANLAGGLSSDDPGLDLGICAAILSSYREIVIPPGFAFIGEVGLTGEV
                     RSPGAVSARIRELSSLGYKTIVLPEAAAKEVGKGGAKEKSVTLRAMQQITELGAVVGA
                     R"
     gene            complement(1346..2692)
                     /gene="dnaB"
                     /locus_tag="PKK76_12445"
     CDS             complement(1346..2692)
                     /gene="dnaB"
                     /locus_tag="PKK76_12445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNL69333.1"
                     /translation="MPWDENAERQVLGTLLVRPDLFLQIGTTVAADDFYPEGHKVIYR
                     AIVEMTQKGEAIDPLRVSQYLTDRSLIAQAGGAETLLQIARDALPSGLIFQHARRVHS
                     FGIRRALIKAAREIQEESVSPQEDESVYLRTIEDKILSITQQTSSTDVAPIGEFRDSF
                     GQHVQHLIQARGGLSGLRTNFREFDRMTSGLKGGELIILAARPSAGKTTLAMNIAANV
                     AIREKKAVLVFSLEMSANQLLGRLMCAESGLNHSDYQRGQMHGKEQTFLAAMNRVFDA
                     PMHLCESGYLELLEAIALTRKLHLQLKGEGKSLGLIVVDYLQLLNDSAARRQSRQLEV
                     ASISRALKQLSKQVDAPVLALSQMNRSVESRRGDGGRPQLSDLRESGAIEQDADIVMF
                     IHRELNPDPNDPEAMENRGKAEIILAKHRNGPVGSFRLAFRPEMNRFDDLDPEYNG"
     gene            complement(2711..3271)
                     /gene="rplI"
                     /locus_tag="PKK76_12450"
     CDS             complement(2711..3271)
                     /gene="rplI"
                     /locus_tag="PKK76_12450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNL69334.1"
                     /translation="MKVILQKDVPGLGDAGEVKNVADGYARNYLLPRKLGTAARGDST
                     KVLEHQKRMMQRKIDKRNKEMADVAEKLKALGTVSISVRVGAKKKLFGSVTPQAVANA
                     LKDLGYPVDKRKVELTDAVRTLGNFKFRVRLAEKVIVPMALEVLADKDSKVEEEEYVP
                     PAVPQTEAAAPEAAAEAKPEKSKKSK"
     gene            complement(3296..3745)
                     /gene="ssb"
                     /locus_tag="PKK76_12455"
     CDS             complement(3296..3745)
                     /gene="ssb"
                     /locus_tag="PKK76_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNL69335.1"
                     /translation="MASDLNRVVLVGRLTRDPELKSVGSGSTLCKFSLASNRTYTAGG
                     EKREEVSFFNCTAWGKQAEILSQYARKGKQLAIEGRLQQRSWDGPDGKKQSAVDIVVE
                     NFQFLGAAGGRDDSSRGGGEYSPQPAGVRQEDAGFASGDYPDDDIPF"
     gene            complement(3735..4016)
                     /gene="rpsF"
                     /locus_tag="PKK76_12460"
     CDS             complement(3735..4016)
                     /gene="rpsF"
                     /locus_tag="PKK76_12460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNL69336.1"
                     /translation="MRNYHIIVVIKDAPDTEELKKGVIQLLGRHEAKVSEDQKWGSRR
                     LPHGMQHEHVGIYYCLTAQMDAGQVKELSHDLNIQSGVLRFMVSRADGK"
BASE COUNT          993 a         1221 c         1117 g         1122 t
ORIGIN      
        1 cctcgcacca acgacggcac cgagctccgt tatttgctgc attgcgcgca gcgtcacact
       61 tttctctttt gctccgccct tgccgacttc ttttgcagcc gcctccggca gaacaatggt
      121 cttgtatccc agggaagaaa gctcccgaat ccgcgcggac acggcaccgg ggctgcgaac
      181 ttctccggtc aaaccaactt ctccaatgaa tgcaaagcct ggcggaatga cgatctcgcg
      241 gtaagaagac aggatcgcgg cgcatattcc aagatcgagc ccgggatcgt ctgaactcag
      301 gccgcccgca agattggcaa aaaggtcaaa ctcagacagt ctcagcttca gatacttttc
      361 gagtaccgcg ccgagcagaa tcaaccgccg attgtctagc ccttccgcca tgcgtctggg
      421 tggaccggcg tagcagcgcg aaacaagcgc ctgcacctcg acacagatgg agcgcgaccc
      481 ctcttgcaaa acagaataca ctctacctgg cgccgggttg tcttctctgt gaggcgcaag
      541 actcaaaacc gaatgcaagc ccgcgttgcc catttcaaag agcgccacct ctccaatggg
      601 tccaaagcga ttcttcacag cgcgcagaat tctatgatgt ttcaggcgat cactctcgaa
      661 caacaaaaca gtatccacca tgtgttcgag caatctcggg cctgcgatgg ctccatcttt
      721 agtgatatga cctgtaatca gaataggaac gctgcaagac ttggccgctt ccataaggcg
      781 aaatgccgct tcgcgcaaaa tagcaggtga cccggggctt gatccagcag actgaactgt
      841 ctggatggaa tcaatgactg caatgtccgg cttatgcttt tctatttcct gagcaattgt
      901 ctcaaggtcc gtttcgcgcg aaacttgaac gttagacgac atgactccaa gacgagtggc
      961 ccggatgcga atctgccctg gagattcctc ccctgaaaaa tagagaaact taccctcaaa
     1021 gcgtcttgcg atttcaagaa ggagtgtgct cttccctaca ccgggttccc ctcccagaag
     1081 aacgagagct ccaggcacaa gcccgccgcc gagtacgttg tccaggtcct ggatgcccgt
     1141 tgtgattctt gcgatgtctg catttgcttc tgaaagagat tcgaaactgg catgcccttg
     1201 agccgcgcgc ttccccggca ccagaccagg ctgaagctct gactctacca tagtattcca
     1261 ttcaccgcat gtgccgcatt ttccttccca gcgagaagac ttcgcgccac aggaggtgca
     1321 ttcgtaagat ttgcgcggct ttttttcagc cattgtattc cggatcgagg tcatcaaatc
     1381 ggttcatttc aggcctgaac gcgaggcgaa aacttcctac aggaccgttt ctgtgcttgg
     1441 ccagaatgat ttccgctttg ccgcgatttt ccatggcttc tggatcattt ggatccggat
     1501 tgagttccct gtggatgaac attacaatat ctgcatcctg ttcaatggcg cctgattcgc
     1561 gaaggtcaga aagctgaggc cttcccccgt ctccgcgtct ggattcaacg gatctgttca
     1621 tctgggagag agcaagcaca ggcgcatcca cctgtttgga cagctgtttg agagcgcgag
     1681 aaatggacgc aacttcgagc tgtctgctct gtctgcgtgc agcagaatcg ttgagcaatt
     1741 gtaagtaatc tacaactatc aaaccaagcg attttccttc gcccttgagc tggagatgaa
     1801 gctttctcgt gagtgcaatc gcttcgagta gttccaggta accggattcg cacaaatgca
     1861 tgggtgcatc gaatactcgg ttcatggcag caagaaacgt ctgttctttt ccgtgcatct
     1921 gaccgcgctg gtagtcgctg tgattgagac cggactcagc acacatgagt ctccccagga
     1981 gctgattggc actcatctcc agagaaaaaa caagcactgc ttttttttcg cggatggcaa
     2041 cgttagccgc tatgttcatg gccagagtcg ttttacctgc agaaggacgc gcggcgagaa
     2101 tgatgagttc tccgcctttc aggccggaag tcatgcgatc aaattcgcgg aagttcgtgc
     2161 gaaggccgga gagaccaccg cgggcctgaa tgaggtgctg cacatgctgt ccaaaactgt
     2221 cccggaattc tccgatgggt gcaacatctg tggacgacgt ctgctgggtg atggaaagaa
     2281 tcttatcttc tatggtgcga agataaacgg attcgtcttc ctgcggagaa acggactctt
     2341 cctggatttc gcgcgcggct ttgatcagtg cgcgcctgat tccaaacgaa tgcactctgc
     2401 gcgcatgctg gaagatcaaa ccggaaggga gcgcatcgcg cgcaatctga agaagagttt
     2461 ccgcgccgcc tgcctgtgcg atgagacttc ggtctgtgag gtattgagat acacggagag
     2521 ggtcgattgc ctctcccttc tgagtcatct ctacgatggc tctatagata actttgtgac
     2581 cttctggata gaagtcgtct gcggcaactg tggtgcctat ttgcaggaag agatccggcc
     2641 gcacgagaag cgtgccgagc acctggcgct cggcattctc atcccacggc acgctcacag
     2701 gattgaccta ttacttggac tttttagact tttctggctt tgcttctgca gctgcttccg
     2761 gagctgctgc ttctgtctgt ggaactgcag gcggaacata ttcttcttct tccactttgc
     2821 tgtccttgtc tgcaagaact tcaagagcca ttggaacgat tactttttca gccaggcgaa
     2881 cgcggaattt gaaattcccc agtgtgcgaa ctgcgtctgt gagttctact ttgcgtttgt
     2941 ctaccgggta acccagatct ttgagagcgt ttgcaactgc ttgcggagtt acagatccga
     3001 agagtttctt ttttgcacca acgcgcactg aaatggaaac tgttccaaga gccttgagtt
     3061 tttcggctac gtctgccatc tctttgttgc gcttgtcgat cttacgctgc atcattcttt
     3121 tctgatgctc gagtactttg gttgaatcgc cgcgtgctgc agttcccagt ttgcggggaa
     3181 gaaggtaatt gcgagcgtaa ccgtccgcaa cgttctttac ttcaccggca tcgcccaggc
     3241 ccggcacatc tttttgaaga attactttca tctcatgctc cctttactct ttcaatcaaa
     3301 acggaatatc atcgtctgga taatcacccg aagcgaagcc tgcgtcttcc tgtctgactc
     3361 ccgcaggttg aggcgaatac tctccgcctc ctctgcttga atcgtcacga ccgcctgctg
     3421 cgccaaggaa ctggaaattt tctactacga tatctaccgc agattgtttc tttccgtccg
     3481 gaccgtccca gcttctctgc tgcaacctgc cttcaatggc gagctgcttg ccctttctgg
     3541 catactgact gagaatctca gcctgcttgc cccaggcagt acagttaaaa aaacttactt
     3601 cttccctctt ctctccgcct gcagtgtatg tacgattgct tgctagagaa aatttgcaaa
     3661 gcgtgctacc agaaccaaca gacttgagtt ctggatcacg cgtgagtcgc ccgacaagga
     3721 cgactcgatt gagatcactt gccatccgct cttgagacca tgaatcgcag aacgcctgac
     3781 tggatgttca gatcgtgaga caattccttc acctgacctg catccatctg agccgtaagg
     3841 caatagtaga ttccaacgtg ctcgtgctgc attccgtgag gcaaacgtct agaaccccac
     3901 ttttgatctt cgcttacttt tgcttcgtga cggcccagaa gttgaatgac gcccttcttg
     3961 agctcttctg tatcaggcgc atccttgatc acgactataa tatgataatt tctcataggt
     4021 accgggcaat ttccgggacg gcctggacct gtcaagaaat tggtttttag agggcctgga
     4081 agaggcaaaa agggccgagt tcgacccggc cgaaggcttc ggccgtggat ccggtcgtgg
     4141 ctgtccgacc agggcgaccc ttccccggcg cccgcggttc tacatttcga ggcgacccga
     4201 ggccaaacgg gtgccgcgcg cccgtcggtt ttatcacgca atagaagtgc gtattacata
     4261 cgaatgcagg cattaacacg aacgttttgt tcgttttaca tcggggggaa gatatgaggg
     4321 cgaacgggtg ccggcgcggc ccggatttat cacgcaataa aagtgcgtat tacatacgaa
     4381 cgcacgcatt aacacgcacg ttttgttcgt tttacatccg ggggaagata tcgcaaaacg
     4441 ttaaatttct ctc
//
LOCUS       DAORFF010000313         4449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11397_length_4449_cov_3.463132, whole genome shotgun sequence.
ACCESSION   DAORFF010000313 DAORFF010000000
VERSION     DAORFF010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11397_length_4449_cov_3.463132"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PKK76_12465"
     CDS             complement(<1..304)
                     /locus_tag="PKK76_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNL69337.1"
                     /translation="MKKTVSIALISMLGLTQITCAGMSSKVPIAMAIGCASGFGLGAI
                     YDEVARNKDAKEKKSVQDRALGIFKNKKKTYNQGKFVGLGVGCLAGLGTGLYLDMMA"
     gene            331..1395
                     /gene="dusA"
                     /locus_tag="PKK76_12470"
     CDS             331..1395
                     /gene="dusA"
                     /locus_tag="PKK76_12470"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: protein motif:HMM:NF008774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HNL69338.1"
                     /translation="MDRQSLSSAASTKISDRRISLAPMMEVTDRHFRRMLRFLSRDAH
                     LYTEMVHSRAVLADARRYLAHMSAEYPLVLQLGGDDPMELEEASRIAVDMGYSEINLN
                     CGCPSERVQRGGFGACLMAEPVLVSQIVSAMKKSGASITVKHRIGIQNIRTDEEPDYD
                     ALRLFVGALADAGCERFIVHARTAILTGLSPAENRSVPPLRFDLARRLKNDFPHLPFE
                     INGGIADLETAGALLEEFDGVMIGRAVRDNPMLFSGVDRVLSAVRQSPENRAELTPHD
                     LLHAMIPYVERSPDSAHIVARNLLNIFHGYPGARRYRRFLSEELPDTDHKSVADLLVE
                     ATRYIEKKAERSEEPVPSSV"
     gene            1493..1876
                     /locus_tag="PKK76_12475"
     CDS             1493..1876
                     /locus_tag="PKK76_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69339.1"
                     /translation="MKPTWKKILAVLMERFPEIESVVFARVKPGALERSAFSPFLLMA
                     FYLDEYSPVRRLRELELALIRLFYLEEEKIRFAAGGGSSQALFPITELMERVREKGAL
                     VYKRARAPRISARELLAIQLMNSAA"
     gene            complement(1922..3241)
                     /locus_tag="PKK76_12480"
     CDS             complement(1922..3241)
                     /locus_tag="PKK76_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNL69340.1"
                     /translation="MLSIETTSRLTELQRLEREYRQIPFEAILKQDVLRLGLAFEESS
                     LKGASFKAKDYFIFTFDHVPLSEQDAGIRFKVPEEIRVEGGPFQLLPTVISVRVGPDS
                     PYVVKGTPEGPALFLGEERIGSVGFPPVPAWYDRKTASGKLPGEVAPVIEWGYLIYLT
                     AFRNCQYFGKDEECAYCDINHNYRQQKGEGRPYTGVKDVSDILEVLSWIAETDTNRIA
                     KAYTITGGSVTTQLRGKGEVDFYLEYASAIESKFPGRWIGKIVTQAWEKEEVRKFKDA
                     GIRIYHPNYEVWDPELFKKICPGKERTIGRDTWIRRILESTEVMGPENVIPNFVGGVE
                     LAKPYGFSTVDEAIQSTAEGLDFFMSHGVAPRFTTWCPEPFTTLSTLSDQTPPPLEYF
                     CKLLTVWKETHEKYKLPVPPGYGDPGPGRAVFSVSAFMDIIGYEGRN"
     gene            3329..4138
                     /locus_tag="PKK76_12485"
     CDS             3329..4138
                     /locus_tag="PKK76_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69341.1"
                     /translation="MAAEIRRSPRIRILLWTRKARQLIMERLTALVPEENRAQVPLLI
                     SVLDELAKNAIKANHKFLLIRRKMREHFRKEGLELAEADRRMESICADAASYNEFLDQ
                     HPGLLDGVQPHLRDILNQEALWINVRNNPERTEAVIAKEKEAMKKTKDYRNLFRDVRD
                     RRVYVEFRASVKPPHLWVEIINTAPILNRDLDRIREKREEFKKHREANTEHEFFMNHM
                     DTSDGGSGLGYATIDTTLASLGHEPLDALYILSLHSTNVMMNLDLGRMAQA"
     gene            4142..>4449
                     /locus_tag="PKK76_12490"
     CDS             4142..>4449
                     /locus_tag="PKK76_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69342.1"
                     /translation="MEQIKKLLTFPKPFLTVYPRVWIVLFFVFVAVDLASKVWITDTL
                     TYRVTEHQLENAVAGHVQMLVHGVGQINILGEEGKYIKLALVFNDRFVFGTGPAAPVF
                     G"
BASE COUNT         1071 a         1120 c         1146 g         1112 t
ORIGIN      
        1 ccgccatcat gtccagatac agaccggtgc ccagacctgc cagacatcct accccgaggc
       61 caacgaactt gccttgattg tatgttttct ttttgttttt gaagatacca agagcgcgat
      121 cctgtacgct tttcttttct ttcgcgtctt tgttacgcgc aacttcatcg tagatggcgc
      181 caagaccgaa accggacgca catccaatcg ccatggcgat tggtactttg ctgctcatgc
      241 cagcgcatgt gatctgcgtc aggccaagca tagagatgag tgcaatgctc accgtttttt
      301 tcatttgatt tcctccatta aaagggccgg gtggacagac aatccttgag ttcagctgca
      361 tcaaccaaaa taagcgaccg tagaatcagt ctggccccca tgatggaggt gaccgacagg
      421 catttccgca gaatgcttcg gtttctgtcc agggatgctc atctctacac tgaaatggtt
      481 catagcaggg cggtcctcgc agacgcccgg cgctacctcg cgcatatgag tgcagaatac
      541 cctctcgtgc ttcagctcgg tggagacgat ccgatggagc ttgaagaggc gtctcgcata
      601 gcagtggata tgggctattc tgaaatcaat ttgaattgcg gatgtccgag tgagcgcgtg
      661 cagcgcggcg gatttggcgc ctgcctgatg gcagagccag tccttgtttc gcagattgtg
      721 tctgccatga agaagtcagg cgcttccatt acagtgaagc acaggattgg aattcaaaac
      781 attcgcacag acgaagagcc ggactatgac gccttgcggt tgtttgtcgg agcgcttgca
      841 gatgcaggat gtgaacgatt catcgtgcat gcacgcactg cgatcctgac agggctttct
      901 ccggcggaga atcggtctgt gcctcctctt cgtttcgatc tcgcgcgaag actgaaaaat
      961 gattttcccc atcttccatt tgaaatcaac ggaggcatcg cagatctgga aactgcaggc
     1021 gcccttttag aagagttcga cggcgtgatg attggtcgcg cggtgagaga taatccaatg
     1081 cttttttcag gagtcgatcg ggttctctct gcggttcgcc agagtcctga gaatcgcgca
     1141 gaactcacgc cgcatgatct gctgcatgcg atgatcccct acgtagaaag atctccagac
     1201 tccgctcaca ttgtggctcg caatttgtta aatatcttcc atggttaccc gggggcacgt
     1261 cggtatcgtc gtttcctgag tgaagagctt ccggatacgg atcataaatc agtggccgat
     1321 ttgcttgtgg aagcaacgcg ttatatagag aaaaaagcgg aacgatccga agagccagta
     1381 ccttcctcag tatagcgcaa cctgcgcgga ggaagaaatg aatgcacttg atataccaga
     1441 attaagaggg agaaatccgg gcagtgggca ccgttgtgtg tttcgagcag agatgaagcc
     1501 aacatggaaa aaaatcctgg cagttctcat ggagcgcttt ccggagattg aaagtgttgt
     1561 gtttgcacgt gttaagccag gggcccttga gaggtctgct ttttctcctt tcttgctgat
     1621 ggcattctat ctcgatgaat attcgcccgt tcgcaggctc agagaactgg aactcgctct
     1681 cattcgcctt ttctatctgg aagaagagaa aatcagattc gcggcggggg gggggtcttc
     1741 ccaggcactt tttcccatca cagaactcat ggagagagtt cgtgaaaaag gggctctggt
     1801 ttacaagagg gcgcgcgctc cgcgcatttc agcgagggaa ctccttgcaa ttcaactgat
     1861 gaactctgct gcctgaatcc cgccgattga atttccgttt cttgatttca acatttcgaa
     1921 atcagtttcg gccctcatat ccaatgatgt ccataaacgc gctgactgag aagacagcgc
     1981 gcccagggcc tggatcgcca tagccaggag ggacgggcag cttgtatttt tcgtgcgtct
     2041 ctttccatac cgtcaatagc ttgcaaaagt attcgagagg cggaggggtc tggtccgaga
     2101 gagtggacag agttgtgaat ggttccggac accaggtggt gaatcttggt gcaacgccat
     2161 ggctcatgaa gaaatcaagt ccctctgcag tagattgtat ggcttcatca acagttgaga
     2221 aaccataagg ttttgcaagt tccactccgc ccacaaagtt tggaatcaca ttttcagggc
     2281 ccatcacttc cgtggactct agaatgcgtc ggatccatgt gtcgcggcca atcgttcttt
     2341 ccttgcccgg acaaatcttc ttaaacagtt caggatccca tacctcgtag ttcgggtggt
     2401 agattctgat tcctgcgtcc ttgaatttgc gaacttcttc cttctcccag gcctgcgtta
     2461 caatctttcc gatccatctg ccggggaatt tggattcaat cgcggatgcg tattccaggt
     2521 aaaaatcaac ttctcctttt ccgcgcaatt gagtggtgac acttccgcct gtgatcgtgt
     2581 aagccttcgc tatgcgattc gtatctgttt cagcaatcca cgaaagtact tccagaatat
     2641 ctgatacgtc ttttactccc gtgtaaggtc tgccttctcc tttttgctga cgatagttgt
     2701 ggttgatgtc gcaatacgcg cactcttcgt ctttgccaaa gtactggcaa ttgcgaaagg
     2761 cagtgaggta aatcaggtag ccccattcaa tgactggagc cacttctccg ggaagtttgc
     2821 cggacgcagt ctttcgatcg taccaggccg ggacaggagg aaatccaaca gagccgattc
     2881 tttcttcccc gaggaagagt gcgggtcctt ccggagtgcc tttcacaaca tacggagagt
     2941 ccgggccgac tcgaacggag atcacagtgg gtagaagctg aaacggtcct ccctcgactc
     3001 gtatttcctc gggaactttg aaacgaattc cggcgtcctg ctctgacaga ggtacatggt
     3061 cgaatgtgaa tatgaaataa tctttagctt tgaaggaagc tcccttgaga gacgactctt
     3121 caaaggcgag accaagccgc agtacgtcct gtttgaggat ggcttcaaaa gggatctgac
     3181 ggtattctct ctccaggcgt tgcagctctg tcaggcgaga agtggtttcg atcgatagca
     3241 tgtcttttca acaaaagacg cgctgccgga ctgacaagta gaaatccctt ttcccttgac
     3301 aacacgtcat tctaaacgag tctgcgccat ggcggcggag attcggcgtt cccccaggat
     3361 tcggatccta ctctggacca ggaaggctcg ccagctcatc atggagcgcc tgacagccct
     3421 cgtcccagag gaaaatcgcg cccaggttcc actcctcatt tcagttctgg atgaactggc
     3481 aaagaacgca attaaggcaa accacaagtt ccttctgatt cgccgcaaga tgagagaaca
     3541 ctttcgtaaa gaaggtctgg agctggcgga agcggacaga aggatggaat ccatctgcgc
     3601 ggacgctgcc agctacaatg aattcctgga tcaacacccc gggttgctcg acggtgtgca
     3661 gccccacttg agggatatcc tcaatcagga agcgctctgg atcaacgttc gtaacaatcc
     3721 agaaagaaca gaagcagtca ttgcgaaaga aaaagaagca atgaagaaga ccaaagacta
     3781 tcgcaatcta tttcgcgatg ttcgggatcg tcgtgtgtac gtcgagtttc gcgcttccgt
     3841 gaagccacct catctatggg ttgaaatcat caacactgct cccattctaa accgagatct
     3901 ggacaggatt cgggaaaaga gggaagagtt caagaagcac cgcgaagcaa acacggagca
     3961 tgagtttttc atgaaccata tggatacctc ggacggtgga tccggtctcg ggtatgccac
     4021 aatcgatact actctggcaa gcctggggca cgaacctcta gacgcacttt acattctatc
     4081 tctgcatagc acaaacgtaa tgatgaacct tgatctcggt cgcatggccc aggcctaatc
     4141 catggaacaa attaaaaagc tgctcacttt cccaaaaccg ttcctaaccg tttacccgcg
     4201 agtgtggatc gttctgtttt tcgtattcgt tgcggtggat ctagcatcca aagtctggat
     4261 tacggataca ctgacctatc gcgtgacaga gcaccagctt gaaaacgcag tggccggaca
     4321 tgttcagatg ctcgtccatg gagtcgggca gatcaatata cttggagaag aaggaaaata
     4381 catcaagctg gcgctggtgt tcaatgatcg ctttgtgttt ggaactggcc ctgccgctcc
     4441 tgtgttcgg
//
LOCUS       DAORFF010000314         4449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11398_length_4449_cov_3.447656, whole genome shotgun sequence.
ACCESSION   DAORFF010000314 DAORFF010000000
VERSION     DAORFF010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11398_length_4449_cov_3.447656"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..908)
                     /locus_tag="PKK76_12495"
     CDS             complement(54..908)
                     /locus_tag="PKK76_12495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL69343.1"
                     /translation="MGKSQTAAPEHTAVRVALWRALHVRIDAEPHVFVDEIGEKIIGE
                     ENWRARADMAPEFSKSMRASVTGRARFIEDIVEEKVNEGVTQYVILGAGIDTFAQRRG
                     DLASRLQVFEVDQPGPQMWKRQRLAELEFDLPASLHFVAVDFEAGESWKDELLKAGFD
                     KSKPTIFASTGVSMYLSRDANAETFGQIARLAPGSIFVTTFMLALELLNAQERSIMEF
                     VMNKARESGTPFLSLFAPEEIVSLAKSSGFRTAEYVSASEIFDRYFAGRSDGLRAGDA
                     EAFLVAST"
     gene            1255..3237
                     /locus_tag="PKK76_12500"
     CDS             1255..3237
                     /locus_tag="PKK76_12500"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL69344.1"
                     /translation="MILSRRIVILCFALAIACRPAGQTPGVRHGQIDLRGFNAEDVVI
                     PLDGDWEFYWDQTERARTEPPEFLPVPGSWVDRGVHPPAGKAVYRLRVLSNQTGRVFA
                     LKIYEFPEAYRLYINGEKVLENGKYAEDPAQSARTLVRPVVTFPLKETNDIIFEVVNL
                     NEDEPGPRRSILLGTERAIRGVQENQIITDMITCGVLLIMGLYHLALYYQRRKENGAF
                     VFGLYCLIMAFRLLVTEEHYLHKYFPGFPGWLEQRLDVMSFFILPPVIAGIFSNFFRR
                     DFHPNLLRFIVWPCATLMAIYCIFPNRLIFETYILLSLVIGIYLGYVLVLGVRRSRRG
                     ALLFLVGWLIYLGTAILDFLSFNNIVRTIYVSHIGFLFFVFSQAYFLSLRFNRALATA
                     EELSERLELRVRERTRELNHSLEIIQKDLAMAREVQESLLKRKGPGYPGLVIVEKYIP
                     FSEVGGDIYDIREIAPGKVRIFVADANGHGVQAALITVMIKSEYDSLNQAQLRPAEII
                     ESFNRSYTDSRRSYVSMFSCFILDLDFEHRTIEYSSGGHPAQMLLRAGEILRLKPGGP
                     MIGIKGNARFGHGVLPLLPGDRFLIFTDGIFEEFNDRKEIFGEERLWASVGALSHQGM
                     SELTVSLLAEVQSFLGNAKWDDDTTILGIEVRIPET"
     gene            complement(3234..>4449)
                     /locus_tag="PKK76_12505"
     CDS             complement(3234..>4449)
                     /locus_tag="PKK76_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69345.1"
                     /translation="YKFTSEEFGSLLGDYEAGLEILLSEAETHPDTDDARKLVSQAAG
                     PLREYANHALRKYRYDWVFDYWDRMRKIKKDIPIEAEFASAMALQAHGAFAEGKYDEA
                     IRIFSDMAQISREGSYGKLVMASYHPEFYEKIVRRAKVLETMQVKPEYTAKVFSLRVG
                     LLRFKWKENGLNGEPPTEVSSSPLKLRKGDVELADMAEESLRRTVLAMSDGKLDLVFD
                     RLALIPPLDVVLPDGQKLPNGITQSPSTSGDDIIDRAAGEVGDLLRERTKAADVVILF
                     WWGTSPLTQHGGSRSFKTGSGKSWRGNINIGVQFYHYDPGLDYMNCGACPGPNGIGFY
                     PHVYMHEFFHVVESRFGIQPVHGFTMPQKFPAWKGSDEVDYYWWQIGQNIPSLKKPGR
                     RGNWSDFSFRER"
BASE COUNT         1085 a         1250 c         1034 g         1080 t
ORIGIN      
        1 gccgcgcgag aattgagttt cgcgtttcct gacgtccgcg gtaccacgac agatcaagtg
       61 ctagccacga ggaaagcctc cgcgtctcca gccctgagcc catccgaacg gcctgcaaaa
      121 tagcgatcga aaatttcact cgcagacacg tactccgccg ttctaaaacc actgctcttc
      181 gccagagata caatttcttc gggagcaaac aaactcaaga atggtgtgcc gctctccctg
      241 gctttattca taacaaattc cataatggat ctctcctggg cattcaaaag ctcaagggca
      301 agcatgaagg ttgtcacaaa aatcgaccca ggagccaggc gcgcgatctg accaaatgtt
      361 tccgcatttg catctctgga caaatacata gaaacaccgg tagacgcaaa aatcgtcggt
      421 ttacttttat cgaaccctgc cttgagtagc tcatccttcc acgattctcc cgcttcaaaa
      481 tcgacagcaa cgaaatgcag cgacgcaggc aggtcgaact cgagctctgc gagtctttgt
      541 ctcttccaca tttgaggacc gggctgatcg acttcaaaca cttgcaggcg cgatgccagg
      601 tctcctctgc gctgcgcaaa ggtatcaatg cccgcaccaa ggatcacata ctgcgtcacg
      661 ccctcattca ccttctcttc tactatatct tcaatgaaac gagctcgacc ggtcacagaa
      721 gcacgcatgc tcttcgaaaa ctccggggcc atatccgcgc gcgccctcca gttttcttcg
      781 ccgatgattt tctcaccgat ctcatctaca aaaacgtgcg gctccgcatc gattcgcaca
      841 tgcaatgcac gccataatgc caccctcacc gctgtatgtt ccggagctgc tgtctgtgat
      901 ttgcccatcc aataccgcct ttgatttatc ccgcaatcaa aggacgcaat aactacacgt
      961 ccgcgtacta acacgcactt tttatgcgtg ataaaacaac gacctggttg cgcgggctat
     1021 gcgcgggcga cgcgcaaacg tgagaatggg tggactgtgg ccaacaaaag cgactaacgt
     1081 gccttgcgat ctgcccaaaa tgaagcaagc gaaaggccac atttacgcac gccccccttt
     1141 gactgcgatc agggttgcca tctcgtctgg cgcaggccag gggctgcccg caggcgcatt
     1201 cgagggctta ctttttgagt tgctccacaa aagctgttgc ccagtgtctg aaccgtgatt
     1261 ctgtcccggc gcatcgtaat tctctgcttt gcgcttgcaa ttgcctgtcg ccctgcaggc
     1321 cagactcctg gagttcgaca cggacagatc gacttgcgtg ggttcaacgc agaagatgta
     1381 gtcatccctc tcgatggaga ctgggaattt tactgggacc agacagaacg cgctcgaaca
     1441 gaaccgccgg agttcctacc ggtgcccgga tcctgggtgg atcgcggagt gcacccgccg
     1501 gccggaaaag cagtctatcg cctgcgcgtt ctttccaacc agactggcag agtgttcgct
     1561 cttaaaatct atgagttccc ggaggcatat cgcctctaca tcaatggaga gaaagttctc
     1621 gaaaatggaa agtacgcgga agatccggcg cagagtgccc gcacactcgt ccggcctgtg
     1681 gtcaccttcc ctctaaaaga aacgaacgac atcatcttcg aagtagtaaa tctaaacgaa
     1741 gacgaacctg gaccgcgcag atccattctg cttggaacgg agcgcgcgat tcgcggcgtc
     1801 caggaaaatc aaatcatcac agacatgatc acgtgtggtg tactcctcat catgggcctg
     1861 taccatcttg cgctctacta tcaaagaaga aaggagaatg gcgcgttcgt gttcgggctc
     1921 tactgtctca tcatggcctt tcgacttctg gtgacagagg aacactacct tcacaaatat
     1981 ttccccggat ttccgggatg gctggagcaa aggctcgatg taatgtcttt cttcatcctc
     2041 ccacccgtca tcgcaggaat cttttctaac ttctttagaa gagactttca cccgaacttg
     2101 cttcgcttta tcgtctggcc ttgcgccact ctcatggcca tctattgtat attcccaaat
     2161 cgactcatct ttgagactta catcctgctt tcgctcgtca ttggaatcta cctgggatat
     2221 gtcctggtgc tcggagtgcg gcgatcgaga agaggtgcgc ttttgttcct ggtaggctgg
     2281 ctgatttatc ttggaactgc gattcttgac tttctttctt tcaacaacat cgttcgcacg
     2341 atttacgtaa gtcacattgg attcttgttc tttgtctttt cacaggctta cttcctttct
     2401 ctgagattca acagagcact tgcaactgcg gaagaattga gcgaacgcct cgagctacga
     2461 gtcagagaac gcacgagaga actcaatcat tccctggaaa tcattcagaa agatctggcc
     2521 atggcgcgag aagtccagga aagcctcctg aaacgcaaag gtccaggata tccgggtctc
     2581 gtaatagtag agaaatatat accattttct gaggtgggcg gggatatcta cgacatacga
     2641 gagattgctc cgggcaaggt tcgcatattc gtcgcggatg caaacggaca cggagtgcag
     2701 gcggccctta tcacggtcat gatcaaaagt gaatacgaca gcctcaacca ggcacaattg
     2761 cgccccgcgg aaatcatcga atcgtttaac agaagttata cggacagtag acgatcctac
     2821 gtatccatgt tctcctgctt catcctcgat ctggactttg aacacagaac cattgagtac
     2881 tcttccggcg gacatccagc tcagatgctt ttacgggccg gagaaattct tcgcctgaaa
     2941 cctggcggcc caatgattgg aatcaaaggc aacgcaagat ttggccacgg cgtgctgccg
     3001 cttctacccg gggatcgctt cttgattttc acagacggca ttttcgagga attcaacgac
     3061 cgcaaagaaa tctttggcga agaaaggctc tgggcctctg tgggagctct gtcccatcag
     3121 ggaatgtccg agctgactgt ttcccttctg gcagaagtgc aaagttttct tggaaacgcg
     3181 aagtgggacg atgatacaac gatccttgga atcgaggtgc gcatcccgga aacctagcgc
     3241 tccctgaaac tgaaatcgct ccagttacca cgacgcccgg gcttcttcaa tgaaggaatg
     3301 ttttgaccga tttgccacca gtagtagtcc acttcatcgc ttcccttcca cgctgggaat
     3361 ttttgaggca tagtaaaacc gtgaacaggt tgaatcccaa aacgcgattc aactacgtga
     3421 aaaaactcat gcatgtacac atgcggataa aagccaatcc cattgggccc aggacaggcg
     3481 ccgcagttca tgtaatccag cccaggatca tagtgataga actgcactcc aatgttgata
     3541 tttcctctcc agcttttgcc tgaaccagtc ttgaaagacc tgcttccacc gtgctgagtg
     3601 agcggactgg ttccccacca gaacaaaata acaacgtcag ccgcttttgt tctttcgcgc
     3661 aggagatcgc ccacttcccc cgcggcgcga tcaatgatgt cgtctcctga cgtggaaggg
     3721 ctttgcgtaa ttccattcgg aagcttctgg ccatctggca gcaccacgtc taaaggtgga
     3781 atcaatgcaa gacgatcaaa aaccagatcg agtttcccat cgctcattgc aagaaccgtt
     3841 ctgcgaagag actcttccgc catatctgca agctccacat ctcctttccg caacttcaga
     3901 ggagaactgc tgacttctgt gggtggctcg ccgttcaggc cgttttcctt ccatttaaac
     3961 cgcaacagcc caactctgag ggaaaaaact ttcgcggtat actccggctt gacttgcatg
     4021 gtttccagga ccttcgcacg acgcacaatc ttttcgtaga attccggatg atacgatgcc
     4081 atcacaagct ttccatacga tccttcacgc gatatctgcg ccatgtctga gaaaatgcga
     4141 atcgcctcat catattttcc ttctgcgaat gctccatgag cctgcagcgc catagctgat
     4201 gcaaactccg cctcaatcgg aatgtctttc ttgatctttc gcattcgatc ccagtagtca
     4261 aatacccaat catatctata cttgcgaaga gcgtgattcg catactctcg caaaggtccc
     4321 gccgcctgtg acacaagctt tcgcgcatcg tctgtgtcag gatgcgtctc tgcttcagat
     4381 aagagaatct caaggccggc ttcatagtcg ccgagcaggc ttccaaattc ctccgacgtg
     4441 aatttgtac
//
LOCUS       DAORFF010000315         4447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11410_length_4447_cov_3.888661, whole genome shotgun sequence.
ACCESSION   DAORFF010000315 DAORFF010000000
VERSION     DAORFF010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11410_length_4447_cov_3.888661"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PKK76_12510"
     CDS             complement(<1..390)
                     /locus_tag="PKK76_12510"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNL69346.1"
                     /translation="MNLAGATLRLWLASGHAEYSRFSAHAADPMKDQTKNLGRIFRLM
                     RSTKFASEHHLGDLDETSTPERLLGALRQVPIRSATEYEPWLADEAETAHILCAKTPD
                     FFEETSGSVRPKLIPYTRELRREMDIAI"
     gene            complement(387..1052)
                     /locus_tag="PKK76_12515"
     CDS             complement(387..1052)
                     /locus_tag="PKK76_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69347.1"
                     /translation="MFQLLEENYSSVSREIFYEDLGSKDFVGLLMDHANIIQGFTTIA
                     LRTRHPDYNVLFSGDTIITPKHWGSQELVRGFCNAAGSFLATSTKPLYWFLISKGHRT
                     YLYLPLFTRKFFPDPNSQNEDLAAIAKTESMLMFPQYYDQEQGLLVFPESRGELRPEL
                     IAATLFRETNAYIRFFRERNPGFVRGNELVCITELSPSNLKGIAKRWLVRGMSSNVIQ
                     AAV"
     gene            complement(1109..1687)
                     /locus_tag="PKK76_12520"
     CDS             complement(1109..1687)
                     /locus_tag="PKK76_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69348.1"
                     /translation="MMAAMLLIFQAAAPLFYLKQKSGKAVGGPISMVKSFWLIFAITL
                     WILLPSILSFTAPGELRICFQLLAVSMILRGVVELYLCYGTIRWKTEYGIAHDSLQIG
                     ICITLLTTRSLPPVHALIILLTILSLITEMYFVHRFRISTSGPAQGVYFVPETDKRTN
                     RLTLLIFAPQYMAFWTLVFVLEYPLIQARIGL"
     gene            complement(1701..2696)
                     /locus_tag="PKK76_12525"
     CDS             complement(1701..2696)
                     /locus_tag="PKK76_12525"
                     /inference="COORDINATES: protein motif:HMM:NF039236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNL69349.1"
                     /translation="MIEAFRPIENYWYIACFSRELKSKPVSRTILGKPLVLFRTGEKP
                     ACLEDRCPHRNIALSLGKVKKDGLQCSYHGWTFGADGKCLRIPAESESGARPAARSLP
                     AVESQGFIWVFLGDKVPGPVPEFPMFSAPRWQNWFMEREFDGSAFQCIENFLDVPHTV
                     FVHSGLFRTREGSDTTLSIVRNNQSVTAEFLNERQVDGLVGKLLVPAGSQAKHTDAFL
                     MPSTTRVDYYFSEKRHFIVMSQCTPVDEYRTRVYTYMAFRFDPIAPIVRLIYAPLSSM
                     ILNQDVRVIKKQSDNIKRFGREAFYFHQTDAIAREIREMMSGRKARSSHKKRIRA"
     gene            complement(2693..>4447)
                     /locus_tag="PKK76_12530"
     CDS             complement(2693..>4447)
                     /locus_tag="PKK76_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNL69350.1"
                     /translation="PPGLHFTRIASSQSGIGYEKSNILEQVDPRVQHIAKWLLSVGDS
                     VASADVDLDGKPDLFFTQSLKSTEYRGKLYLNQGNFQFKKIVIPDLERYLNDPAKFGL
                     PAFAFFFDYDNDGDQDLFVGFGYGRSHMFENRIKQEGIVRFREIETQATKDHTIALAA
                     NAFDFNRDGRLDVLVANTLPVYLPDYLPEKIPLNIFKLPEPRYEGDRRMLHFMHHSWH
                     NANNGGKNELYLGTKGPDPFSRTDVGLDETRWSLSVGTADLNRDGFTDIYIANDFGRD
                     DCYINDGGKRFRRQQGEFFEDLGLDTYKGMNASMGDIDGNGADDVYVSNVHHPMQAEG
                     SLLWMNYTASGAQNLSMKDRASQRGALNPHRFGWGAAMADLDLDGKLDIVQANGMVSN
                     KWDKRYATCLDYWYLNEKLARSIPEIHTYADNWADLRGACIYPDEADRIYINQGSRFS
                     DFADDVGVTHRANTRGVAAVDLDNDGDLDLVFTDQFGEPIIYRNDINKPSWAGLILHG
                     NGRTCNRDAVGSTVELKSPSISQHREVRLVNGFSAQSDTRLLFAVTGSARAVIQWCGQ
                     EIQEIDLPAGRYTTLEQR"
BASE COUNT         1067 a         1116 c         1091 g         1173 t
ORIGIN      
        1 aatggctatg tccatttccc gtcggagttc gcgcgtatat gggatgagct tgggccgcac
       61 ggagcctgat gtttcttcaa agaagtcggg cgtctttgcg cagaggatgt gtgctgtttc
      121 tgcctcgtcg gcaagccagg gttcgtattc tgtcgcactt cgaatcggca cctgtcgcag
      181 cgcgcctaag agtctttcgg gcgtggaggt ttcatccaga tcacccaggt gatgttcgct
      241 cgcaaatttc gtcgaacgca tgagtcgaaa gattcgcccc agattttttg tctgatcttt
      301 cattggatcc gctgcgtgcg cggagaatcg ggaatattca gcatgccccg aggccagcca
      361 gagtctgagt gtcgcaccgg ctaaattcaa actgccgcct gaattacgtt agacgacatt
      421 cctcgaacca gccacctttt ggcgatccct ttcaggttcg atggagaaag ctctgtaata
      481 catacgagtt catttccccg cacaaagcca ggatttcgct cgcgaaagaa tcgaatgtac
      541 gcatttgttt ccctgaagag agtagcggca atgagttccg gcctgagctc tcctctgctt
      601 tcgggaaaca caaggaggcc ttgttcctgg tcatagtatt gtggaaacat aagcatggat
      661 tctgtttttg cgatcgcggc cagatcttcg ttctgcgagt tcggatccgg aaagaatttc
      721 ctggtgaaca aaggtagata cagatacgtt ctgtggccct ttgaaattag aaaccagtag
      781 agaggcttcg tgcttgttgc cagaaaactt ccagcagcat tgcagaaacc tctcacaagt
      841 tcctgtgaac cccagtgttt gggcgtgatg attgtgtctc ccgagaaaag gacgttgtaa
      901 tctggatgcc ttgtgcgcag tgcaatggtt gtgaaaccct ggatgatgtt tgcgtggtcc
      961 atgagcaatc caacaaagtc ctttgaaccc aggtcttcgt agaatatctc gcggcttaca
     1021 ctggaatagt tttcctccag cagctggaac atcctttccg attcctgatg cgaaatggat
     1081 gctcttttac gcaggcagaa gctgagactc acaagccaat cctggcctga atcaacggat
     1141 actccagcac aaaaaccagc gtccagaaag ccatatactg cggggcgaaa ataagcaacg
     1201 tcaggcgatt agtccgcttg tctgtttccg gtacgaagta aacaccttga gccgggcccg
     1261 aggttgagat acgaaatcgg tgaacgaaat acatttcagt gatcaggcta agaatggtaa
     1321 gtaagatgat cagtgcatgg accgggggca gagaccgcgt ggttagcaga gtaatgcaga
     1381 tcccaatctg caaagaatcg tgcgcaatcc catactccgt tttccatcgg atggtcccgt
     1441 agcaaagata caattcaacg acgcctcgca agatcatgct cactgccagg agctgaaaac
     1501 aaatgcgcaa ctcgccgggg gcggtgaagg atagaatgga tggtagtaga atccagagtg
     1561 tgattgcgaa aatcagccag aaggatttga ccatggagat tggtccaccc actgctttac
     1621 ccgatttctg cttcaggtag aacagaggag ctgcagcctg gaatattaga agcatcgcgg
     1681 ccatcatagc gcattcctct tcatgccctg attctctttt tatggctgga cctggctttt
     1741 cgcccgctca tcatttccct gatttcacga gcgatcgcat ccgtttgatg aaagtaaaaa
     1801 gcttctcggc cgaatctttt gatgttgtca gattgtttct tgatcacgcg gacatcttga
     1861 ttgagaatca tcgaagaaag gggcgcatag atcaacctga cgataggcgc tattggatca
     1921 aatcgaaagg ccatgtaggt gtagactctc gtccggtact cgtctactgg agtgcactgg
     1981 ctcatcacga taaagtgcct tttttcagag aaatagtaat ccactctagt agtagagggc
     2041 atgagaaagg catccgtgtg ctttgcctgg cttccagccg ggaccagtag tttcccaacc
     2101 aggccgtcta cctgtcgttc gttcaggaat tccgcagtca ccgattggtt gtttcgcact
     2161 atggatagag ttgtatcaga accttcgcgg gtgcgaaaga gtccagaatg gacgaacact
     2221 gtgtgcggaa cgtccaggaa gttttcaatg cattggaacg cacttccatc gaattctctt
     2281 tccataaacc agttttgcca tcgcggggct gaaaacattg gaaactctgg aaccggtccc
     2341 ggaactttat ctcccaggaa gacccagatg aaaccttgac tttctactgc tgggagactg
     2401 cgcgccgccg gtcgcgcgcc cgattcagat tcggccggga tgcgaaggca tttgccatct
     2461 gcaccaaaag tccagccatg atagctgcat tgcaggccgt ctttcttgac tttgcccagc
     2521 gaaagggcaa tgttgcggtg aggacaacga tcttccagac acgccggctt ttctcccgtg
     2581 cgaaatagca ccagaggttt gcccagaatc gttcgcgaaa caggttttga cttaagttcg
     2641 cgcgaaaaac atgcaatgta ccaatagttt tctattgggc gaaatgcttc tatcatctct
     2701 gctccagggt cgtataacgt ccagctggca gatctatttc ttgaatttcc tggccacacc
     2761 attgaatgac tgcgcgggca ctgcctgtaa cggcaaatag caacctggtg tccgactgtg
     2821 cggaaaatcc attgaccagt cgtacttctc tgtgttgcga aatagatggc gatttcagtt
     2881 ccaccgtgct tccgaccgcg tctcgattgc aggtccttcc gtttccgtgc aaaatcaagc
     2941 cagcccagga cggtttattg atatcgtttc tataaatgat tggctctcca aattgatctg
     3001 tgaacacgag atctagatct ccatcgttgt ccaggtccac tgccgccact ccgcgcgtat
     3061 ttgcgcgatg agtaacgccc acgtcgtctg cgaaatctga aaaccgggat ccctgattga
     3121 tatagattcg atccgcttca tcaggataga tgcatgcgcc tcgaaggtcc gcccagttat
     3181 cagcgtaggt atgaatttcc ggaatggagc gtgccagttt ttcgtttaga taccagtagt
     3241 ccaggcaggt cgcataacgt ttgtcccatt tattagagac cattccgttt gcctgcacaa
     3301 tgtccagttt accgtccaga tccagatctg ccatggctgc gccccatccg aacctatgcg
     3361 gattgagtgc gccacgttgc gacgcgcgat ctttcatgga aagattttgc gccccggatg
     3421 ctgtgtagtt catccagagg agacttccct ctgcctgcat gggatgatgc acattggaaa
     3481 cgtatacatc gtctgcaccg ttgccgtcta tgtcccccat gcttgcattc attcccttat
     3541 atgtatccag gcccaggtct tcaaagaatt ctccctgttg tctgcgaaaa cgtttgccgc
     3601 catcattgat gtagcagtcg tctctaccaa aatcatttgc aatgtagatg tccgtgaaac
     3661 catctctgtt cagatccgca gtccccacgc tgagactcca acgcgtttca tccaatccaa
     3721 cgtctgttct ggaaaaagga tccggacctt ttgtgcctag atagagttcg ttctttccac
     3781 cgttgtttgc gttgtgccat gaatgatgca tgaagtggag catgcgtctg tctccttcat
     3841 aacgcggttc tggaagtttg aagatattga gtggaatttt ctctggcaaa tagtccggca
     3901 gatatacagg caaagtattt gccaccagca cgtccagtct accgtctcga ttgaagtcaa
     3961 acgcattggc tgcaagagca atcgtatgat ctttcgttgc ttgagtttct atttcgcgaa
     4021 agcgaacaat gccttcttgc ttgattctgt tttcaaacat atgacttctt ccgtagccga
     4081 atccaacgaa caaatcctga tctccgtcat tgtcgtagtc gaagaaaaat gcgaaagcag
     4141 gtaagccgaa ctttgccgga tcgtttagat atctctccag atccgggatg actattttct
     4201 tgaattgaaa attcccctgg ttcaagtaca actttccgcg atattccgtc gatttcagac
     4261 tctgcgtaaa gaagagatcc ggtttcccgt ccaggtccac atctgcggat gcaaccgaat
     4321 caccgactga taaaagccat ttcgcaatgt gctgaacgcg tggatctacc tgttccagaa
     4381 tgttgctttt ttcatacccg attccggact gagacgacgc gattcttgtg aaatgaaggc
     4441 ccggagg
//
LOCUS       DAORFF010000316         4446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11419_length_4446_cov_3.301070, whole genome shotgun sequence.
ACCESSION   DAORFF010000316 DAORFF010000000
VERSION     DAORFF010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11419_length_4446_cov_3.301070"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PKK76_12535"
     CDS             <1..323
                     /locus_tag="PKK76_12535"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNL69351.1"
                     /translation="RSGELLTGHALIRNDRGQRRTLVKVQRTVVRFAQLEDSEIEAYV
                     DAGEAMKCAGAFALEGRGGLFVEGIEGCHSNVIGLSLPLLRKMMLELGAAPHEFWVSS
                     AEEL"
     gene            320..1489
                     /locus_tag="PKK76_12540"
     CDS             320..1489
                     /locus_tag="PKK76_12540"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HNL69352.1"
                     /translation="MKSENFETIAVRTQAERSGFREHSAPIYMTSSFMFEDAEQARAL
                     FADEIEGNIYSRFSNPNTTEFIQKICKLEGAEDGIATASGMSAIFTSMMPFLSSGDHI
                     IVSRSVFGSTHQIVTQILPRWGIEYTYVDILDTVGWEKAFRKNTKMVFVETPSNPALD
                     MIDLAWLSGLCKSHNVLLNVDNCFATPYLQKPISFGADIVMHSATKFIDGQGRTIGGV
                     IVGKKEIIAKVRFFARHTGPSMAPMTAWILSKSLETLAVRMDRHCQNALALARFLETH
                     PKVEKVIYPFLESHPQLELAKKQMRLGGGIVTFLVRGGIEAGRKFLDATEGLSFSANL
                     GDTRTIVTHPASTTHSKLSDEERAAVGIHPGTIRVSAGLEHIDDIISEIDRMIKA"
     gene            complement(1479..2390)
                     /locus_tag="PKK76_12545"
     CDS             complement(1479..2390)
                     /locus_tag="PKK76_12545"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNL69353.1"
                     /translation="MLKDKILKRQSGITLYGITPPKASTAQEKLSEISDAQVQRLSSL
                     SIDGLIVYDLQDEGSRTTENRPFPFMETLDPFSYARKYLEKLRVPKVIYRAVGKYSAD
                     ELTHFMEHAPPDEYLSVFVGAASKTQKVNLTMREAYALKKKIKSELLVGGVVIPERHS
                     AHSPEHLRVFDKISSGCKFFVSQGVYNLDASKNFLSDYYYYGKEHKIELVPIIFTLTP
                     CGSLKTLQFMQWLGISIPKWLENDLIHSNDILSRSIELSRNIWLELKSFADAKNIPIG
                     RNIESVSIRKEEIDASVELLKITSATP"
     gene            complement(2421..2831)
                     /locus_tag="PKK76_12550"
     CDS             complement(2421..2831)
                     /locus_tag="PKK76_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNL69354.1"
                     /translation="MEIELTRKNDGYLFEAKNESGNTVMIDASPAIGGSGGGARPMEV
                     VIMALGGCSSIDIVNILKKQRQEITSFQVKIHAERREGETPSLFKSIHVDYYIGGVDS
                     DKAQRAADLSIEKYCSVSKLLEPTVKITYAVHSV"
     gene            2862..3395
                     /locus_tag="PKK76_12555"
     CDS             2862..3395
                     /locus_tag="PKK76_12555"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNL69355.1"
                     /translation="MSTECARQREEPMLESYWPFYYGGLAIAAVALLVLSIGGDYLAI
                     TRGYVSVCSIFTRRPYFNTPDIGGPFGFRTMFTIGVVVGGFIAAIVSGGFKPTFAFGT
                     FDSVWGTSMAVKGAVLVSGGICWGYGSRMAGGCTSGNSISGISRGSLASIVATVCFLV
                     GGAIVVHILEYVVRGKL"
     gene            3395..3796
                     /locus_tag="PKK76_12560"
     CDS             3395..3796
                     /locus_tag="PKK76_12560"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNL69356.1"
                     /translation="MLRLFYLLAGVAFGFVLIYSGVSNYNVLYDMFLFRSFHMYGLLG
                     VAVGLSFVGVQILKKLKWKSVITGQDLKFETLMPTRAHVIGGLISGAGWGLTGACPGP
                     ALAQVGFGTLAGIFTVCGIFIGVYLFGRGKG"
     gene            3799..4146
                     /locus_tag="PKK76_12565"
     CDS             3799..4146
                     /locus_tag="PKK76_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69357.1"
                     /translation="MPGAWSGFAQAIVKGVRAAGAGLHYVYRKGDEHKHYVLPAVNGL
                     VGDKLAEANHPRAIQMSFRVDGRDCSVDDPAIASKIDKSKTVVIFVHGLMADEVLWQE
                     PTAGKSGFGPRLD"
     gene            4171..>4446
                     /locus_tag="PKK76_12570"
     CDS             4171..>4446
                     /locus_tag="PKK76_12570"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69358.1"
                     /translation="MRYNSGRHISTNGRELAGLLEEFVKCHGKKVKRLVIIAHSMGGL
                     VSRSAGYYGTRAKHKWISLLTNVVLVGVPNDGSYLEKFGHLATFVLKR"
BASE COUNT         1035 a          993 c         1181 g         1237 t
ORIGIN      
        1 gtagaagtgg tgagcttttg accggtcatg ctttgattcg aaacgatcgc gggcagcgca
       61 gaactcttgt aaaagtgcag cgtacagtgg ttcgattcgc acagcttgag gattcagaaa
      121 ttgaggcata cgtggatgcg ggtgaggcaa tgaagtgcgc cggtgctttc gctctggaag
      181 gaagaggcgg actttttgtt gaaggaattg agggttgtca ctctaacgta attggcctga
      241 gtctaccgct cttgcggaag atgatgctgg aactgggcgc ggcgccccat gagttctggg
      301 tgagttctgc ggaggaatta tgaagagtga aaattttgaa acaatcgcgg tgcgcactca
      361 ggcggagaga agtggatttc gcgagcattc tgcgcccatt tacatgactt ccagttttat
      421 gttcgaagat gcagagcagg ctcgcgcttt gtttgcggat gaaatagaag gaaatattta
      481 cagcagattc tccaacccca acacaaccga attcattcaa aagatatgca aactggaagg
      541 ggcagaagac ggaatcgcca ctgcttccgg aatgtcagcg atcttcacaa gcatgatgcc
      601 ttttctaagc tcgggggatc acatcattgt atcgcgatct gtgtttggtt ctactcatca
      661 gatcgtaact caaattctac ctcgctgggg aatcgaatac acctatgtgg atatcctgga
      721 cactgtgggc tgggaaaagg catttcgcaa gaataccaaa atggtttttg tggaaactcc
      781 atccaatcct gctctcgaca tgattgacct ggcatggctc tccggcctgt gtaagtcgca
      841 taacgttctg ctcaatgtgg acaattgttt tgcaacgcct tatctacaga aaccgatttc
      901 gtttggtgcg gacattgtga tgcattcagc caccaagttc atagacgggc agggaagaac
      961 gatcggcggc gtcatcgtag gcaaaaaaga aatcatagca aaggttcggt tcttcgccag
     1021 acacacaggt ccttccatgg cgcccatgac tgcgtggatt ctttcgaaaa gcctcgaaac
     1081 tctcgcggtg aggatggacc gtcattgcca gaatgcactg gcacttgctc gttttctcga
     1141 aacacatcca aaagtggaaa aagtcatcta tccattcctc gagtcgcatc ctcagctgga
     1201 gttagcaaaa aagcagatga ggctgggtgg tggaatcgtt acgtttctag tgcgcggcgg
     1261 aattgaggca ggccgcaaat tcctggacgc aaccgaaggt ctttctttct ccgcaaatct
     1321 gggagacacg cggacaatcg taactcatcc tgcttctaca actcattcga aactatccga
     1381 cgaggagcgg gcggctgttg gtattcatcc tggaacgatt cgagtttcag cgggccttga
     1441 acacatagac gatatcatat ccgagatcga tcgtatgatc aaggcgtagc gcttgtaatc
     1501 ttcaaaagct caacagaggc atctatctct tccttccgga tggaaacact ctcaatgttt
     1561 cttccaatcg gaatgttttt ggcatcagcg aagcttttca actcaagcca gatgttcctg
     1621 gacaattcaa tggacctgga aagaatatcg tttgagtgga tgaggtcatt ttcaagccat
     1681 tttggaatgc tgatcccaag ccattgcatg aattgcagtg tcttcaaaga gccgcacgga
     1741 gtcagcgtga agataatcgg cacaagttcg atcttatgct ctttgccata gtagtaatag
     1801 tctgacagaa agtttttgga tgcgtctaag ttgtatacgc cctgggacac gaagaatttg
     1861 cacccggatg agattttgtc aaagactctc agatgctcgg gcgagtgggc ggaatgcctt
     1921 tcagggatga ctacaccgcc gaccaacaac tcgctcttga ttttcttttt caaagcgtaa
     1981 gcttctctca tcgtcaggtt tactttttgt gttttggaag ccgcgccaac aaagaccgac
     2041 aggtattcat ccggcggagc atgttccatg aaatgagtaa gttcatccgc gctgtattta
     2101 cctaccgccc tataaatgac ttttgggact ctcagttttt ctaagtactt ccgagcgtag
     2161 gaaaaaggat cgagggtctc catgaatgga aagggccgat tctctgtggt tctagatcct
     2221 tcatcctgca aatcgtaaac aatgagaccg tcaatagaga gggaactcag tctttgaact
     2281 tgcgcgtccg aaatctcgct gagtttttcc tgagctgtag atgcttttgg tggggtgatc
     2341 ccgtagagtg taattccgct ctggcgttta agtattttgt ctttcagcat gattgcggtt
     2401 tgtttatcgc ataactcctg ttaaacggaa tgaaccgcgt atgtgatttt cacggttggt
     2461 tcgagcagtt ttgatacgga gcagtacttc tcaatagaaa gatctgccgc tctttgtgcc
     2521 ttgtctgagt ccacgccgcc aatatagtaa tctacatgga tggatttgaa gagggatgga
     2581 gtttcccctt cgcgtctctc cgcgtgtatt ttgacctgga agcttgtgat ttcctgccgt
     2641 tgttttttga gtatatttac aatgtctatt gagctacagc cgcccagggc cataatgacc
     2701 acttccatgg gtcgtgcgcc gccacctgaa ccgccgatgg ctggagatgc gtcgatcatg
     2761 actgtgttgc cagattcgtt tttcgcctca aaaaggtagc catcgttttt tcttgtcagt
     2821 tctatttcca ttgtaatcct tccatattcg cgaccgggca ggtgtcaacc gaatgcgccc
     2881 ggcaaagaga ggagcctatg cttgaaagtt actggccgtt ttactacggc ggcctggcca
     2941 tcgcggccgt cgcccttctt gtcctatcca ttggaggcga ctaccttgcg atcactcgtg
     3001 gatatgtgag tgtttgttcg atctttacac gtcggcccta tttcaacacg cccgatattg
     3061 ggggcccgtt tggctttcgc actatgttta cgattggagt cgtggtggga ggattcatcg
     3121 ctgcgattgt atcgggtggt ttcaaaccaa ccttcgcatt tggcactttt gattcggtct
     3181 ggggaacgtc gatggcagtg aaaggtgcag ttcttgtttc gggaggaatc tgctggggtt
     3241 acggatctcg catggcggga ggatgcacgt ctggaaattc aatttcagga atttcgcgtg
     3301 gttctcttgc gagcattgtt gcgaccgttt gtttcctcgt gggaggagca attgtagttc
     3361 atattctcga atacgtggtg cgggggaaac tgtaatgttg agactcttct atctacttgc
     3421 cggcgttgcg tttggttttg ttctcatcta ctccggtgta tccaattaca atgttctcta
     3481 tgatatgttt ctgtttcgca gctttcacat gtatggcctg ctgggtgtag ctgtaggcct
     3541 ttccttcgtg ggcgttcaga ttctaaaaaa actcaaatgg aaatcagtga tcacaggcca
     3601 ggatcttaaa tttgaaactc tcatgccaac acgtgctcat gtgattggtg gactgatctc
     3661 aggtgcaggc tggggtttga ccggagcatg ccccggaccg gccctggcac aggtaggttt
     3721 tggaacgtta gccggcatct ttacagtgtg cggaattttt atcggagtgt atctgttcgg
     3781 cagaggaaag ggctaatcgt gccgggcgcg tggtctggat ttgcgcaagc gattgtaaag
     3841 ggagtccggg cggctggcgc gggacttcac tatgtttatc gaaaaggaga tgaacataag
     3901 cactatgttc tgcccgcagt caacgggctc gtaggtgata agctggcaga ggcaaatcat
     3961 cctcgcgcca ttcagatgag ctttcgcgtg gatggccggg attgctctgt ggatgacccg
     4021 gccatcgcga gtaagataga taagagcaaa accgtagtga ttttcgtgca tggtctaatg
     4081 gcagatgaag ttctctggca ggagcctact gcaggcaaat ctggattcgg gccacgtctg
     4141 gactaagacg gtcgattttg ctgtttctat gtgcgctata acagtggaag acacatttct
     4201 acgaatggtc gcgagcttgc aggactgctc gaagagttcg tgaagtgcca tggcaaaaaa
     4261 gtgaaacgac tcgtcatcat tgctcattcg atgggtggtc tggtttcgcg ctctgccggg
     4321 tattacggaa cacgcgcgaa acacaaatgg atctctcttc ttaccaatgt agtactggtc
     4381 ggtgtgccca atgacggatc ctatctggag aaatttggac atctggcgac ttttgttttg
     4441 aaacga
//
LOCUS       DAORFF010000317         4443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11431_length_4443_cov_3.966727, whole genome shotgun sequence.
ACCESSION   DAORFF010000317 DAORFF010000000
VERSION     DAORFF010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11431_length_4443_cov_3.966727"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PKK76_12575"
     CDS             complement(<1..945)
                     /locus_tag="PKK76_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNL69359.1"
                     /translation="MGRLSTLFFLILIHESCIHRSRPEFPGVLLDQASHDFLFTRPDV
                     YDPSIRDRIVHEIDGAKESISLYCYEIDEPEILRALDRARGRKILLKIVGSPDQSYAE
                     LRESGFAFRIRARSGLQHSKVWIIDHRLLISGTGNFTQSDLFHNNNAFFFLNVSTNTA
                     MEVDRSLENADANASPVFLPYGGVLLVSPSRGKLIQTRLILAALNAKKTLRYMIFSHT
                     DQVLSSAMAESAMRGVLIEGIYDAESDGLPDEGVQLNIATRAFIYTDGNRSEFQTEDG
                     VVHGGHLHHKTMVVDDEMVLTGSYNFSIGARDSNQEAYF"
     gene            complement(986..2380)
                     /locus_tag="PKK76_12580"
     CDS             complement(986..2380)
                     /locus_tag="PKK76_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69360.1"
                     /translation="MKSFIRFFSSSFLMPAFLHVAASVFVSDTLYAIENDDPVYAGRS
                     PLSVSFLAQGIRSSVYPDRLKDALEFSSDELSAGVHVKGSSLDAEENSFLYSADAAAL
                     FSAQGKVQLLRGSLFAAGYSGCSASGCGIMLGQFYPDPRGSVGVDPIGGNAHSSHASF
                     LGDAEDGRSGTAVFFRGEHAGVEFYPAIVDLNNAEARGARAQAFLFFKNAGMSVHYEG
                     IQQGSQAGKGKDRLDYTGAGFYIDKPDGFIQFYTRIAVIHSQGRYTTMHTGETHSTRA
                     SLEGQALKAGAMFSVGWFHLYADGFLPEPPSNVDGNRTSDEKSGYTGWNQFSGTNIFL
                     REIDAVPFARLCRAKENCTGIESSTSFRAFRFAAGVARARVSLEMPRLELSLAGSLFR
                     PLAPRTDHSGNPYSKIRRDHKIPELREITVQVSIQHEVGRLLFEYGRLSAKSDEGSKL
                     AAERFTLSLERAAF"
     gene            complement(2377..>4443)
                     /locus_tag="PKK76_12585"
     CDS             complement(2377..>4443)
                     /locus_tag="PKK76_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69361.1"
                     /translation="DSRFMILETSDPRIVAPFLSGLSRVPGITNNLRGSADVYVSEVK
                     QGKDAFLEISSRTAGRAKLRILRGASVENLELYFFGGSAYVFDHADAVELPSGVFSIT
                     ADQYETAGGNATRSVTVRDEHLVPTACLQLCDSRGISSFPSGTSDDACKPEDLELTEL
                     SPAGVYTDHVDASGKFLEVRVKRSCMLGSVLLRAGSTIDPGQGKVESGARLLFASSSE
                     FFSEKVLDADLRSLNYSTPVEAFDSEGHAAILYSPDADARWADSSGFVHSIVLDQGVA
                     IYHDRSRVGLRPDLTSNAMSPGLPEISEAPHSSFSLSEVSLMGSWNGSSVPDDEFIEL
                     TGKEGGPGRWTVDVKRLSDGKTIRHHFPPQDGIAAYFRKSPVCFPGYFGRADLFLPNE
                     PVELSWFDSHGENQGHASVSPALATSMSSAYASAVWHESTHSLRVHSPIVGICAKANA
                     SPGAPGSPSPFFAEEPSPAGRIFRFYSDVAASISVEIDGLSSSRMAANEDLISVTALR
                     PRSVFRITASGAQVSGEVLLSPGLAVTGVHPSPVAPGVEWIRVCATAPFFPQTNLYVH
                     DEDSEDRIVPFSQRTGRALLSSTMNLPAFGCALIVDPDYTGQSFPPPPFPFYDYAGWS
                     ISSTSAIGSGLGADESVWITNATGDILATYGLPDIAPFHVHASSGQTIVRTWPSYDAP
                     ESWSAQ"
BASE COUNT         1121 a         1119 c         1170 g         1033 t
ORIGIN      
        1 aaaataggct tcctgattgg aatcgcgcgc tccaatcgaa aaattgtacg accccgttag
       61 aaccatctca tcatctacaa ccatagtctt atgatgaaga tgtccaccgt gcactacacc
      121 gtcttcagtt tggaattcag aacggtttcc atccgtgtat ataaaagctc ttgtggcaat
      181 gtttagctga acgccttcgt ccggcagtcc gtcgctttct gcgtcataga ttccctctat
      241 aagaactcct cgcatagcgc tttctgccat ggcagaggat aaaacctggt ctgtgtggct
      301 gaagatcata tatcgtaatg tttttttagc attgagcgcg gccagaatga gtctggtctg
      361 gatgagcttt ccgcgagagg gcgaaaccag aagaactcct ccgtatggaa ggaatactgg
      421 agatgcattt gcgtctgcat tttcgaggga cctgtccact tccatggcag tatttgtgct
      481 cacattcaga aaaaagaatg cattgttgtt gtgaaataga tcgctctgcg taaaatttcc
      541 tgtgccagaa atcagcagtc tgtgatctat aatccacact ttggaatgct gcaaacctga
      601 cctggctctg atccgaaagg caaaaccgga ttcgcgcaat tccgcataac tttgatccgg
      661 ggatcctaca atctttagaa gaatcttgcg ccctctggcg cggtcgagcg cgcgaagaat
      721 ctctggttca tctatttcat agcaatacag gctgatcgac tcttttgcac catctatctc
      781 atggacgatt cgatctcgaa tagaaggatc gtatacgtcc ggtctcgtga acaagaaatc
      841 atgcgatgcc tgatccaaaa gaacccctgg aaactccggc cttgatcgat ggatgcagga
      901 ttcatgaatc aaaatcaaaa agaacagtgt tgaaagtctt cccattcctg tactggtatc
      961 cgatgtgcaa atcgttctgc ccgaatcaaa aagcggctcg ctcaagcgaa agggtgaatc
     1021 gttctgcggc gagctttgag ccttcgtccg atttcgcgga cagtcttcca tattcgaata
     1081 gcaatctccc tacttcatgc tggatggaga cctggacagt gatctccctg agctccggga
     1141 ttttatggtc gcgacgaatt ttagagtaag gattgccaga gtggtctgtt cgaggcgcca
     1201 gcggacgaaa cagtgatcct gccagagaaa gttcgagccg aggcatttcc agggagaccc
     1261 tcgcgcgagc cactccagcc gcgaagcgaa aagcgcgaaa ggaagttgat gattcgattc
     1321 cggtgcagtt ttccttcgca cgacaaagcc tcgcgaaagg aactgcgtcg atttcgcgaa
     1381 ggaagatgtt tgttcctgag aactgattcc acccggtgta tcctgacttt tcatcggacg
     1441 tgcgattccc gtccacgttg gacggaggct ctggcagaaa accatctgcg tagaggtgaa
     1501 accatccgac agaaaacatg gctccggcct tcaatgcctg accttctaga gaggcccgcg
     1561 tggaatgcgt ttcccctgtg tgcatggtcg tataacgtcc ctgagaatga atcaccgcaa
     1621 tgcgtgtgta gaactggatg aatccgtccg gcttgtctat gtagaatccg gcgcccgtgt
     1681 aatccagacg gtcttttcct ttacccgcct ggctgccttg ttggattcct tcgtaatgaa
     1741 cggacattcc agcgttttta aaaaatagaa atgcctgggc ccttgcgccc cgggcctccg
     1801 cattgttcag atctacgatc gcaggataga attctacgcc cgcatgttct ccgcgaaaga
     1861 agaccgcagt gccgctcctc ccgtcttctg cgtctcccag gaaagaagcg tgcgaagaat
     1921 gtgcattgcc tccaatgggg tccacgccca ccgatcctcg tgggtccgga tagaattgtc
     1981 cgagcatgat tccgcatcca gaagcgctgc acccggaata tccggcggca aatagagagc
     2041 ctcgaagtag ctggactttg ccttgagcgc tgaagagtgc ggctgcgtct gcggaatata
     2101 gaaacgagtt ttcttctgca tccagagaag agcctttgac gtgaacacca gcactcagct
     2161 catctgagga aaattctaga gcgtctttga gtctgtccgg atagacgctg gatctgattc
     2221 cctgagcaag gaacgacacg gaaagcggtg atctccctgc atagacaggg tcgtcgttct
     2281 caatggcgta taacgtgtcc gaaacgaaaa cggatgccgc cacatgcaag aatgcgggca
     2341 tcaagaacga actggagaag aatcgaatga aagatttcat tgtgcgctcc aggattctgg
     2401 cgcatcatag gaaggccatg tgcgaacgat ggtttgtccg gaggatgcat gcacatgaaa
     2461 aggagcaatg tctggcaggc catacgtggc cagaatgtcg cctgttgcat tcgtaatcca
     2521 gactgattcg tctgcgccca gtccagaacc aatcgcagaa gtggaggaga tggaccagcc
     2581 agcatagtcg taaaacggaa agggaggtgg tggaaacgat tggccggtgt agtcaggatc
     2641 tacgatgaga gcgcatccga acgcaggaag attcatagta gaagaaagaa gagcgcgtcc
     2701 tgtgcgctgc gaaaaaggaa cgattctatc ttctgaatct tcgtcatgaa catagagatt
     2761 tgtttgcgga aaaaacggtg cagtcgcaca gactcgaatc cattctacgc ccggagccac
     2821 gggagaagga tgcactcctg tgaccgcaag tcccggtgac agcaggactt cacctgaaac
     2881 ctgagcgcct gacgctgtaa ttctaaaaac ggacctgggg cgaagcgccg tcactgagat
     2941 gagatcttca tttgcggcca ttctagaaga acttaagccg tctatttcta cagagataga
     3001 agccgcaaca tctgaataga agcgaaagat tcgtccggca ggtgatggct cttctgcgaa
     3061 gaaaggagac ggagaacccg gagcgcctgg acttgcgttc gcttttgcgc agattccgac
     3121 aatgggcgaa tgtacgcgaa gagagtgcgt agactcatgc cagacggcac ttgcgtatgc
     3181 gctactcatg gaagtggcga gtgcagggga tacgcttgca tggccttgat tttctccatg
     3241 agaatcaaac caggagagtt ctaccggttc gtttggaaga aagagatcgg cgcggccaaa
     3301 gtaccctgga aaacagacag ggcttttgcg aaaatatgcg gcgattccat cctgcggcgg
     3361 aaaatgatgc cggattgttt ttccatcaga aagtcgctta acgtctaccg tccagcgtcc
     3421 tggaccacct tcctttcctg ttagttcaat gaattcatcg tctggaacag agcttccatt
     3481 ccagctcccc atcagagaca cttcagatag agaaaagctg gaatgtggcg cttctgatat
     3541 ctcaggaagc ccgggagaca tagcgttgga cgttagatct ggacgaagtc cgactctgct
     3601 tctgtcatga tagatggcaa cgccctgatc caggacaatg gagtgaacga agccggaaga
     3661 gtctgcccat ctggcatctg catctggtga atacagaatg gctgcatggc cctctgaatc
     3721 gaaggcttct actggcgtgc tatagttcaa tgagcgaaga tctgcgtcca aaaccttttc
     3781 tgagaaaaac tcgctggaag aagcgaagag aagccttgcg ccggattcga cttttccctg
     3841 acccggatct atggtggaac ccgcgcggag tagaaccgaa ccgagcatgc acgatctttt
     3901 cacgcgcact tccaggaact ttccggacgc gtccacatgg tctgtgtaca ctcctgctgg
     3961 cgaaagttcg gtgagttcca gatcctccgg cttgcatgcg tcatccgacg tcccggacgg
     4021 gaaggaggag attcctctcg aatcgcataa ctgcaggcag gcggtgggaa ccagatgttc
     4081 gtctctcaca gtaacagacc tggtggcgtt gcccccggcc gtctcatatt gatccgcggt
     4141 gatggagaat actccagacg gaagttctac cgcatctgcg tggtcaaaca cgtaggcgct
     4201 tcctccaaag aaatacaatt ccagattctc tacgcttgct cctcgaagga tgcgaagctt
     4261 cgcgcgtcca gcagttcgcg aagaaatttc taggaaggca tctttccctt gcttcacttc
     4321 gctgacatat acatccgcag atccgcgaag attattggtg atgcctggaa cacgcgaaag
     4381 accgctcagg aacggcgcga cgatgcgagg atcggatgtt tccaggatca tgaacctgga
     4441 gtc
//
LOCUS       DAORFF010000318         4419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11532_length_4419_cov_3.435610, whole genome shotgun sequence.
ACCESSION   DAORFF010000318 DAORFF010000000
VERSION     DAORFF010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11532_length_4419_cov_3.435610"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4397)
                     /locus_tag="PKK76_12590"
     CDS             complement(<1..4397)
                     /locus_tag="PKK76_12590"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNL69362.1"
                     /translation="MPEGIQQVLSRLTAKNPEDRYKSAAGLARDLTECLKQLQEKGTI
                     SNFTAGSRDFSDSFQIPQTLFGREKERDLLLASYAQVCDGQPVLALISGRSGAGKTVL
                     IHEMLRPMTKSHGYFTSGKFEAHKRDVPYSAFIDAFEELIRQLLTESKERLAAWKDQL
                     VRSFGMNGQVLIDVIPDLQLIVGDQAPVRELPPTEARNRFHLVFQSFVRVFATREHPL
                     TLFLDDLQWTDVATLGLLKTLLADPDLRHLFIVGSYRDNEVPDAHPLTLALEDLRKAG
                     IKPVDLKLALLGPRDVRELVIGALALPEGRTSLADDLADAAFRKTDGNPFYCGEFLKN
                     LHQDGLIFFDEENGHFTYDILKIRAQSMSDDLLRIIAGNIQHLSETGQDLIRMSACLG
                     SKFDLRTLAMCMDWTQTKTAGEIEELLIEGLLVSLDDSYKYAAFSGGQSEMQVDYRFV
                     HDQVHNAAYTLLNDDHRRQNHLRIGRTLLANLPFAERDDRILEITHHFNQGRELILDA
                     EERLRLIELNIQAGKKARDASAYEEALRLFGVAFDVLPPDAAEKHYDIYMQVHRDLGE
                     INYILNDPKNADKYFSKILEKAKSPLEKVRVYELKIAMSAGRGERAEAIRLGLEVLGL
                     LGVKLTSSPSASSVKAELGAADKSLSGKNPDRLVDLHLMEEESNKAAMRVLMQIATPA
                     FAGNAPLFYSIIARMIQLSTKHGNAPASAYAYAAYGMILCGDGESERGYRIGRMALDL
                     VEKLQARDLKCRVYFLYSAGVHHWKQHARESTDYLTHAYQAGLESGDLEYVAAVIFLL
                     TASALWVGHKPLKEVLSDFDRFANPLYRTGQKQVIQLVQMGRQYISNLQGGSRNRTRL
                     SGERFHEEETAEFWKETRYGDGLYYLNLFKTSLADMFGQYDQALDFARKAEENGELQK
                     ATLSHPALELHRGLSAGALAGQPKADRKKLLEAFDASMSRIESFAKNSPDNFKHMHLM
                     LRAERARLDARHGDAIPLYDEAIETARSQGFHLDEAHANELAASYFTGLGQRKFALLY
                     VREARAIYGALDITAKVEEIENHFPELVRGSQMSGSGGLAQAGGFDFQTIVKAAASIS
                     GEIQLEGLLGKLLRIVMENAGAQKAHLLLDQEGAVVVSAAGNALTDQVTPVPNVPLED
                     YNLLPHSIVLLAERTRTSIVVDEAASDSRFRADPYILEYKPRSVLCMPVVLQGKLSCI
                     LYLENNGAPGVFSPARLEALQVLSAQIATSLENAKLYSNLERALEQEKLARQAQVEIN
                     EAISRFVPSEFLKLLGKENIVGVSLGDNIAREMTVLFSDIRSFTSLSESMTPEQNFRF
                     INSYLNQVGPVVREHHGFIDKYIGDAIMALFDSPDNALMSSIGMLRALEVWNHGRLSA
                     GYAPVRTGIGLHTGDLMLGTIGEKSRMEGTVISDHVNLASRMEGLTKIYGTSLIISNR
                     TQSLLKDPSRFSFRTLDRVQVKGKKEPVIV"
BASE COUNT          995 a         1108 c         1145 g         1171 t
ORIGIN      
        1 acaatgaccg gttccttttt ccccttcacc tgcacccggt ccagagtgcg aaatgagaaa
       61 cgagatggat cttttaaaag actttgagtg cgattggaaa tgatgagaga cgttccatag
      121 atttttgtca gcccttccat gcgcgaagcc aggttgacgt ggtcactgat cacggttcct
      181 tccatgcggc ttttttcacc aattgtgcca agcatcagat ccccggtgtg aaggccgatt
      241 ccagtgcgaa caggtgcgta accggcactc aatcttccgt gattccagac ttctagagcg
      301 cgaagcatgc cgatcgaaga cataagagcg ttgtccggag aatcgaacag cgccatgatc
      361 gcatctccga tgtacttgtc gatgaagccg tggtgttcgc gcaccaccgg cccaacctga
      421 ttgagataac tattaataaa tctaaagttt tgttccggtg tcatggattc agacagagat
      481 gtgaaggatc tgatgtctga gaagagcacc gtcatttctc gcgcgatgtt gtctcccaga
      541 gagactccga ctatgttttc tttgcccagt agtttcagga attcagaggg aacaaatcga
      601 ctgattgctt cattgatttc tacttgagcc tgtctggcga gtttctcctg ctcgagtgct
      661 ctttctagat tgctatagag ctttgcattc tccagggaag tagcgatctg agccgataga
      721 acctgaagtg cttcgagtct ggccggactg aatactccag gcgcgccgtt attttccaga
      781 tacagaatgc acgataattt cccctgcaat acgacaggca tgcacaggac acttcgcggt
      841 ttgtattcta gaatgtacgg atccgcgcga aaacgcgaat cggaggcggc ctcatctact
      901 acaatgctgg tgcgagtgcg ttccgctaga agaacaatag aatgcggcag caggttgtaa
      961 tcttccagag ggacattcgg aacaggcgta acctgatcgg tgagcgcgtt tccagctgcg
     1021 gaaactacga ccgcgccctc ctgatcgaga agaaggtgtg ctttttgtgc acctgcgttt
     1081 tccattacga tccggagaag ttttccaagc agaccttcga gctggatctc tccggaaatg
     1141 gatgcggcgg cttttacaat cgtttgaaaa tcaaaaccgc ctgcctgtgc aagaccgcct
     1201 gaacctgaca tttgcgatcc gcgcacaagt tcggggaaat gattctcgat ttcctctact
     1261 ttggccgtga tgtcaagtgc gccgtagatg gctcttgctt cgcgcacata cagcaatgcg
     1321 aatttcctct gacccaatcc cgtgaaataa ctggcggcga gttcgtttgc atgagcttca
     1381 tctagatgaa atccctgcga cctggcggtt tcgatagcct cgtcgtagag gggaattgcg
     1441 tcgccgtggc gggcgtccag tcttgctctt tccgcgcgaa gcatcaggtg catgtgtttg
     1501 aaattgtccg ggctgttttt tgcgaatgat tcaatgcgtg acatggacgc gtcgaatgct
     1561 tcgagcagtt tttttcgatc cgctttgggc tgcccggcga gagctccggc agaaaggcct
     1621 ctgtgtaact cgagggccgg gtgagagagt gtggctttct gcaattctcc gttttcttcc
     1681 gccttgcgcg cgaaatccaa tgcctggtca tactggccaa acatgtctgc gagagaggtc
     1741 ttaaataaat tcaagtaata gagtccgtct ccgtagcggg tttctttcca gaattcagca
     1801 gtctcttcct cgtggaatct ctctccggac aacctggttc tgtttctaga accgccctgc
     1861 aaattggata tgtattgcct gcccatttgc acgagctgga tcacctgttt ttgtccggtg
     1921 cgatacagcg gatttgcgaa acgatcaaaa tcggagagaa cttctttgag aggtttgtga
     1981 cctacccaca atgcactggc agtcagaaga aagatgaccg cggccacata ttctagatct
     2041 ccggactcca gtcccgcctg ataggcatga gtgaggtagt cagtagattc gcgtgcatgt
     2101 tgcttccagt gatgcactcc agcagagtag agaaagtaca ctctgcattt caaatcgcgc
     2161 gcctgcaatt tttctaccag gtcaagggcc attctaccaa ttctgtagcc tcgctccgat
     2221 tctccatctc cacacagaat cattccatag gcagcatacg cgtacgcaga cgcaggagca
     2281 tttccgtgtt tggtagaaag ctggatcatt cgcgcaatga tagaatagaa aagtggagcg
     2341 tttcccgcga aggctggagt tgcaatttgc atgagcactc gcatggctgc tttgttggac
     2401 tcttcttcca tgagatgaag atctaccagg cgatctggat tctttccgga gagagattta
     2461 tctgcggcgc cgagttccgc tttcacactc gacgcagaag gcgaagaagt gagtttgact
     2521 ccgaggagcc ccagaacttc aaggcccagg cgaattgctt ctgctctttc tccacggcct
     2581 gcactcatgg cgattttgag ttcgtagacg cggacttttt cgagggggct ttttgccttt
     2641 tcgaggattt tagagaagta tttgtccgcg ttttttggat cgtttagaat gtagttgatt
     2701 tcgcccaggt cgcggtgcac ctgcatgtaa atgtcgtagt gtttttccgc cgcgtcggga
     2761 ggaagaacat cgaatgcaac gccaaacaac cgaagcgctt cttcgtaggc gcttgcgtcg
     2821 cgcgctttct ttccagcctg aatgttcagt tcaatgaggc gcaagcgctc ttccgcatcc
     2881 agaatcagtt ctcggccctg gttaaaatga tgcgtgattt ctaaaatccg atcgtctctt
     2941 tctgcaaacg gaaggtttgc aagaagggtt ctgccaattc gcagatggtt ctgtctcctg
     3001 tgatcgtcgt ttaacagagt gtaggcggca ttgtgcacct ggtcatgcac aaaccgataa
     3061 tccacctgca tttctgactg cccgccggaa aatgcggcat acttgtagga atcgtcgaga
     3121 gatacgagca gaccttcgat cagcagttct tcaatttcac ccgctgtttt agtctgcgtc
     3181 cagtccatgc acatggccag ggtccgcaga tcgaatttac ttcccagaca cgctgacatg
     3241 cgaatcaaat cctggcctgt ttctgacaaa tgctggatgt tgcctgcgat gatgcgaagc
     3301 aaatcgtctg acatgctctg ggcgcggatc ttgagaatgt cgtaggtgaa atgtccgttc
     3361 tcttcatcaa agaagatgag tccgtcctgg tgcaggtttt tgagaaattc tccgcaatag
     3421 aatgggttac cgtctgtctt gcgaaacgct gcgtctgcaa gatcgtctgc gaggctggtt
     3481 cttccttctg gaagtgcgag cgccccgatt acaagttccc gcacatcgcg cggtccaagg
     3541 agcgccagtt tcaaatccac aggtttgatt ccagctttgc gtagatcttc cagggccaga
     3601 gtgagcggat gcgcgtctgg aacttcgttg tctctatagg aaccaacaat gaacagatgg
     3661 cgaagatccg gatctgcgag cagagtcttc agtagcccga gcgtggccac gtctgtccat
     3721 tgcagatcgt cgaggaatag cgtcagagga tgttcgcgcg ttgcaaacac tcgaacgaag
     3781 ctctgaaaca ccagatgaaa acggttacgc gcttctgtgg gtggaagctc ccgaacagga
     3841 gcctgatctc ctactatgag ttgcagatca ggaattacgt cgatgagtac ctggccgttc
     3901 ataccaaacg aacgcacgag ctggtctttc catgctgcca ggcgctcttt gctttccgta
     3961 agtagttgtc ggatcagttc ttcgaatgcg tctatgaatg cgctgtaagg tacgtctctt
     4021 ttgtgagcct cgaattttcc ggaggtgaag taaccatgac tctttgtcat gggccgaagc
     4081 atttcatgga tcagaactgt ttttccggct cccgatctac cggaaatcaa agcaagaacc
     4141 ggttgtccgt cgcacacctg ggcatacgac gcaagcaaca gatctctttc tttctcacgg
     4201 ccgaataacg tctgagggat ttggaatgag tccgaaaaat cgcgcgaacc ggcggtgaag
     4261 ttgctgatcg tacctttttc ctggagttgc ttcagacatt ctgtaagatc gcgcgcaaga
     4321 ccggctgcac ttttgtatct atcttcggga ttctttgcag tcaggcgact gagaacctgt
     4381 tgtatgcctt ccggcactgc gtcgccgtat cttgccttg
//
LOCUS       DAORFF010000319         4415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11551_length_4415_cov_3.324312, whole genome shotgun sequence.
ACCESSION   DAORFF010000319 DAORFF010000000
VERSION     DAORFF010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11551_length_4415_cov_3.324312"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PKK76_12595"
     CDS             <1..556
                     /locus_tag="PKK76_12595"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNL69363.1"
                     /translation="ILFTSRLMLSRVHALLEAKESGISYISQVSLGPPAALRAFHENP
                     DSVLLGLSSFWQGIDVPGDALRLVVIVRLPFQVPDDPVLSARTEKLQNAGRSPFSEIQ
                     LPHAVLTVKQGFGRLMRRESDRGIVSILDARLRTKSYGKDVLAALPRARLVSSFEDLQ
                     KSYQELFSDIQNVAEIPGRPILNR"
     gene            553..1389
                     /locus_tag="PKK76_12600"
     CDS             553..1389
                     /locus_tag="PKK76_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69364.1"
                     /translation="MKRVLFAILLFSSAALLAVEASYGIRKDHLQKTLGEGVYVDWTA
                     GYISVSADHKIEDVRAGGGLTRVRDDATQKAREKALSRLMQIVSTLPIHGHDTIESKL
                     ESDESLQNRMGMLASLPFEKARRVGDGSVSVELILPFYGRRGLLSRVYSGEESEMAEL
                     VHENPEDPITGIVVDATEASSDPVLEPRILTDQGRLIYGPGIARRGCYISRGMAAYYT
                     SREAARKDKRVGENPYLVYARGRKTPGDLFISSQDSVRILGSKSGRDALRKCAVTIVV
                     SK"
     gene            1389..2366
                     /locus_tag="PKK76_12605"
     CDS             1389..2366
                     /locus_tag="PKK76_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HNL69365.1"
                     /translation="MNPARVQQILGSFDKIRILVLGDFMIDEYRYGRAERLSPEAPVP
                     VILETGVRQLPGGAGNVVKNLESLKIKNTALGVCGQDQEAEVMRGLLSGCEELSLIAD
                     RSRPTTKKMRIVAGTQQICRLDQEDASSVPREVEDEIVSRADRMIPSCDAVIISDYDK
                     GVVTERVIESCIRSSHEKVFISVDPQVSRFGFYHGVGVLTPNHHEAGRFIGMRLETDA
                     EVEKGGRLIMQKLSPRMLLITRGEKGMSLFEGDSVRHFPTRAREVFDVTGAGDTVITV
                     FTAVIAAGGTPEEAVFLSNQAAGLVVARLGAATVTREEILFGHEHPERS"
     gene            2344..2838
                     /gene="rfaE2"
                     /locus_tag="PKK76_12610"
     CDS             2344..2838
                     /gene="rfaE2"
                     /locus_tag="PKK76_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNL69366.1"
                     /translation="MNTLSALDKIRAKVFADRHALKRELDQHHSGRTTVLTNGCFDLI
                     HRGHILTLTGARSQGDLLIVALNTDESVRKLKGPTRPVTSQEDRAFVMAALACVDYVT
                     FFGEETPVETIRILAPRVHVKGGDYKPEDLPEREIVLSLGGRLVVLPFEQGYSTTAIL
                     EKSK"
     gene            2844..>4415
                     /locus_tag="PKK76_12615"
     CDS             2844..>4415
                     /locus_tag="PKK76_12615"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017857552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNL69367.1"
                     /translation="MPRTIFITGGVSSSLGKGVTVAALGCLLESRGYRVALQKMDPYL
                     NIDAGTMSPFQHGEVYVTDDGAETDLDLGYYERFTANVKLTRANSVTTGQIYNAVIQR
                     ERRGDYLGRTVQVIPHITNEIKNRVVELTREEDADFVIVEIGGTVGDIESVPFLESIR
                     QMRRERGREGVCFIHLTLVPTITVAGEAKTKPTQHSVKELMTFGIQPDLLVCRAQKPL
                     SDDLKEKLAMFCNVPEGAIVSACDIATSIYEIPEMYKQGGLDLGVLRHFGMKPGKLNF
                     KKWAQILHVINHPRKTVRIAVCGKYIALQDAYRSVYESLMHAGIANQVTVEFVKIDPE
                     DLERTRSTAALKGVHGILVPGGFGSRGIEGKLMSIHYARTKKIPFFGICLGMQCATIE
                     FARNVLGLHGASSTEFDPKTHHPVISLLEEQQGIEQKGGTMRLGAYPCVIKEGTLAAR
                     EYKTTRIRERHRHRFEFTLRYKEAMEKHGLTFSGTSPDGQLMEIIELKSHPWFVGVQF
                     HPEFQSKPIACHPLFA"
BASE COUNT         1141 a         1032 c         1194 g         1048 t
ORIGIN      
        1 cattctgttt acatctcgtc ttatgctttc gcgcgttcat gctctcctcg aagcgaaaga
       61 aagtggaatc tcctacatca gccaggtgag ccttggtccg cccgccgctt tgagagcgtt
      121 tcatgaaaat ccggactctg tcttacttgg cctttccagt ttctggcagg gaattgatgt
      181 tccaggagat gccctcaggc tcgttgtgat tgtaaggctt cccttccagg tgccagacga
      241 tcccgtgctg tctgctagaa ctgaaaagtt gcagaatgcg ggcagaagtc ctttttcaga
      301 aatccagcta ccgcatgcag ttttgaccgt gaagcagggc tttgggcgac tcatgagaag
      361 agaaagtgat cgaggcattg tatcgattct ggatgctaga ttgagaacga agtcctatgg
      421 aaaagatgtg ctggcggctc tgcccagggc gcgactggtc tccagtttcg aggatctcca
      481 gaaatcctat caggagcttt tctctgacat tcagaacgtg gctgaaattc ctggcaggcc
      541 gatattaaat agatgaaacg cgttttgttc gcaatccttc tctttagctc ggcggctttg
      601 cttgccgtgg aagctagcta cgggattcgc aaagaccacc tgcaaaagac tctcggcgaa
      661 ggagtctatg tggactggac tgcgggatac atttctgttt ccgcagatca caaaatcgaa
      721 gacgtgcgtg cgggtggtgg tctcacgcgg gtgcgcgatg acgcaaccca gaaagcaaga
      781 gagaaagccc tttctcgcct gatgcagatt gtttccactc ttccaatcca tggtcatgat
      841 acaatcgaaa gcaagctgga atcagatgaa tccttgcaga atagaatggg aatgctcgcc
      901 tcactgcctt ttgaaaaggc caggcgagtc ggcgacggaa gcgtgagtgt agaattgatc
      961 ctaccatttt acggtcgtcg tggacttctt tctcgcgtgt actcaggcga agaatctgaa
     1021 atggcagagc tcgttcatga aaacccggag gatccgatca ccggaatcgt ggtggatgcg
     1081 acagaagcat cttcggatcc agtactcgaa ccacgtattc ttacagacca gggacgattg
     1141 atttacggtc caggcattgc ccgtcgtggt tgctacatat ccagaggcat ggcagcgtat
     1201 tacacatcga gagaagcggc acgaaaagat aagagagttg gcgagaatcc gtatctggta
     1261 tatgctcgcg gtcgaaagac tccgggcgat ttattcattt cctctcagga ctccgtgcgc
     1321 atccttggct caaaatctgg ccgggacgct ttgagaaagt gcgcggtcac aatcgtagta
     1381 agtaaataat gaacccagct cgcgtacagc agatccttgg ctcgtttgat aagattcgca
     1441 tactcgttct cggtgatttc atgatagacg agtatcgcta tggaagagcg gaacgactct
     1501 ccccggaagc gccggtccct gtgattctgg aaacaggagt gcgtcagctt cctggtggcg
     1561 ctggaaatgt tgtaaagaac ttagaatctt taaaaatcaa gaacacagct ctgggcgtct
     1621 gtggacagga ccaggaagcg gaagtgatga gaggtctgct atcaggatgt gaagagctca
     1681 gtttgatcgc agacagaagc aggcccacta caaagaaaat gaggattgtt gctggaactc
     1741 aacaaatttg cagactcgat caagaagatg catccagtgt gccgcgcgaa gtggaagatg
     1801 aaatcgtttc gcgtgcggat aggatgattc cttcctgcga tgcagtcatc atttcagatt
     1861 atgataaggg agtggtcacg gagagagtga tcgaatcctg catccgttcg tctcacgaaa
     1921 aagtttttat cagtgtagat cctcaagtga gtcgatttgg tttttatcat ggagtaggag
     1981 tactcactcc gaatcatcat gaggcaggca ggttcattgg aatgcgtctg gaaacggacg
     2041 cggaagtgga aaaaggtggg cggctcatca tgcaaaaact ttccccacga atgctactca
     2101 tcactcgcgg cgaaaaagga atgagcctct ttgaggggga ttccgtgcgt catttcccaa
     2161 ccagagctcg cgaagtgttc gatgtaacag gggccgggga cactgtaatt actgttttta
     2221 cggcagtgat agcagctgga ggcactccgg aagaggcagt ttttttaagc aatcaggccg
     2281 caggattggt tgtagccaga ctgggcgctg caactgtaac ccgagaggag atccttttcg
     2341 ggcatgaaca ccctgagcgc tcttgacaaa atccgggcga aggtattcgc agaccgtcac
     2401 gctctcaagc gtgaactgga ccagcatcac tccgggcgca ccacagtttt aacaaacggc
     2461 tgtttcgatc tcatccatcg cggccatatt ctaactctga cgggcgccag atcgcaggga
     2521 gatcttctca tcgtcgcgct caacacagat gaatccgtgc gaaaattgaa aggaccaaca
     2581 cgtcctgtga cttcacagga ggaccgcgca tttgtgatgg cagcccttgc atgcgtggat
     2641 tacgttacgt tctttggaga agaaacacct gtagagacga ttcgaattct cgcaccgcgt
     2701 gtacacgtca aaggtggaga ctacaaacca gaagacttac cggagagaga aatcgttctt
     2761 tccctgggag gcaggcttgt ggtacttcca tttgagcagg gatactctac tacagcaata
     2821 ctggaaaaat caaaatgatc cgaattccta gaaccatatt catcaccggc ggagtgagct
     2881 catctctggg caagggcgtg acggtggccg ctctgggctg tctcctggaa agcagaggtt
     2941 atcgcgttgc cttgcagaag atggatcctt acctgaacat tgatgcgggg accatgagtc
     3001 cttttcaaca cggggaagtg tatgtgacag acgatggtgc agagacagat ctggaccttg
     3061 gatactatga gcgattcact gcaaacgtaa agctcactcg cgcgaactcc gttaccacag
     3121 gtcaaatcta caacgcagtc atccagagag aaaggcgcgg agattatctg ggaagaaccg
     3181 ttcaggtgat tcctcacatc acaaatgaaa tcaaaaatcg cgtcgtggaa ctcacgcgcg
     3241 aagaggatgc ggactttgtg atcgtagaga ttggcggaac ggtaggcgac attgaatccg
     3301 taccgtttct ggaatccatt cgtcagatga gacgcgaacg tgggcgagaa ggggtttgtt
     3361 tcattcatct caccctcgtg ccaacgatca ctgttgcggg agaggcgaag accaaaccaa
     3421 ctcagcacag tgtaaaggaa ctcatgacgt ttggtatcca gccagacctt ctggtttgtc
     3481 gggcgcagaa acctctctcc gacgatctaa aagaaaagct tgctatgttc tgtaacgtgc
     3541 cggagggcgc gatcgtttct gcctgcgaca ttgccacgag tatatacgaa atccctgaaa
     3601 tgtacaagca gggtggcctg gatctgggag tacttcgcca ttttggaatg aagccaggca
     3661 agctcaactt caagaaatgg gcgcagattc tacacgtaat caatcatcct cgcaaaactg
     3721 tgcgcattgc agtttgcggc aaatacatcg cgctgcagga tgcttataga tctgtatacg
     3781 agtctttgat gcatgcagga attgcaaatc aggtcaccgt cgagttcgtt aagattgatc
     3841 cagaggatct ggaaagaacc agatctacgg cggcactcaa gggagtgcac ggaattctgg
     3901 tgccgggcgg atttggaagc cgcggtattg aagggaagct catgtccatt cactacgcgc
     3961 gcacaaagaa gattccattc tttggaatct gcctgggcat gcaatgcgct accattgagt
     4021 ttgcgcgaaa tgttctaggg ctacacggtg caagttccac tgaattcgat ccaaagacgc
     4081 atcatcctgt gatttcgctt ctggaagaac agcagggaat tgaacaaaag ggcggaacca
     4141 tgcgactggg tgcctatccc tgcgtgatta aggaggggac tctcgccgcc agagagtata
     4201 agacgactcg aattcgggag cgtcatcgtc atcgctttga gtttacttta agatacaaag
     4261 aggcaatgga aaagcatggc ctaacttttt ccggaaccag tccggacggc cagctgatgg
     4321 aaatcatcga attgaaatcg catccgtggt ttgtgggtgt gcagtttcat cctgaatttc
     4381 aatctaaacc aatcgcgtgc caccctctgt ttgca
//
LOCUS       DAORFF010000320         4410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11573_length_4410_cov_3.934788, whole genome shotgun sequence.
ACCESSION   DAORFF010000320 DAORFF010000000
VERSION     DAORFF010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11573_length_4410_cov_3.934788"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="PKK76_12620"
     CDS             <1..1043
                     /locus_tag="PKK76_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69368.1"
                     /translation="NADVTKQKEAEQERKKKEKEADAAREAGMRRRFAIVGASAVRGF
                     YQAAFQEQYTQHVLMTVQRGGIFPGAVFTSDLHDRDLKGGEAFGSLTWNRLYFRGSYL
                     SFHAQPKLIQGLMPSSQSGPVLTRDDLTMDLKRSEAGAAAGYSFVRTPTFELRALALF
                     RDEIMNASIRSAGASVSTDSTRPFYGDFNYDDGVRHRAYGHGGGLSAIYNVGKLKLSI
                     DLESYRLHGPWGLKYKKTERSNGSPGFAPLYVDTEGTIRKTTSVLSPAAGYEVFAGFS
                     IRLAARFEQASYQIRRATMPLFLGGASPPDFASALFLQNVVYNGFGFSEKLNRHDSVQ
                     YVSLGIEKKIDF"
     gene            complement(1040..1534)
                     /locus_tag="PKK76_12625"
     CDS             complement(1040..1534)
                     /locus_tag="PKK76_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69369.1"
                     /translation="MRFLENRVVLPTSIPGELKLPAKSALMAHMDRKVESCEKIDDRI
                     TFRGKMYGAGRNPWANCDGEITLSTPGKFILITASISHLRAAFWQLLFYPALGSLTLT
                     LILRYFFGRPSWMVPAIIAALFCIGGIRTIRRAPGRFREAMIDSLRSLPRDSSSWNDE
                     LAGI"
     gene            1650..2123
                     /locus_tag="PKK76_12630"
     CDS             1650..2123
                     /locus_tag="PKK76_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69370.1"
                     /translation="MIFLSCSGAPVQEGAIRGSAGGYTIVFPQKWKIVRPDDVTLEAV
                     SEDELAGVRIVEQGRIGKSAEDILGKIEKQLEVQNSLPESSRTLDANLLKESNAQHCA
                     IGQYALRKNGVLLRRKIWVFVKGQRAFVITTNVPESYAGEYPGVFEGIIKSLRAQ"
     gene            complement(2128..3759)
                     /locus_tag="PKK76_12635"
     CDS             complement(2128..3759)
                     /locus_tag="PKK76_12635"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF005737.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HNL69371.1"
                     /translation="MNIRIIDTKPFADQKPGTSGLRKKVPVFKQEHYLQNFVQSIFDS
                     IGAPPGSALTVGGDGRYFNREAIQIILKMAAANGFSRILVGKDGILSTPAASCVIRKH
                     GTFGGIILSASHNPAGPSGDFGIKYNTSNGGPAPEKVTDAIFAKTKSISQYKWIEVPD
                     VNLGKVGEQTLDDMIVQVIDPVSDYAALMESIFDFDAIRRLFFSGFKMKFDAMHAVTG
                     PYAVEILQKRLGASAGTVMNAVPLEDFGGGHPDPNLTYAHDLVEIAYGKDGPDFAAAS
                     DGDGDRNMILGKGFFVNPSDSLAVLAANATLVPAYKKGLAGIARSMPTSAAADRVAQS
                     LGLACFETPTGWKFFGNLMDAGKVTLCGEESFGTGSNHVREKDGLWAVLFWLNILAVK
                     HQSVETVMREHWNKFGRNFYSRHDYEGIPSEAASGLMKHVTESFAELTGKKFGKHEIR
                     FCDDFSYTDPVDASVSSGQGIRIIFVDGSRIIFRLSGTGTEGATLRLYLEAYEPDPAR
                     HNLDAQEALSDFITIASQISGLRERTGRERPTVIT"
     gene            complement(3825..>4410)
                     /locus_tag="PKK76_12640"
     CDS             complement(3825..>4410)
                     /locus_tag="PKK76_12640"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002751889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL69372.1"
                     /translation="GGARVPATIVFLDIRNSTAIAEKIDPQQFSDFISSIFTDIMDLV
                     YGNHGSVNKLIGDGMLATFGCPIQTEDDKGNALRFALQVREYMNTFNDVRPDYLTEPV
                     RIGIGIASGNVFAGNTGSVRRMEYTVLGDAVNLASRLESLTKLSGVDILIDGVTRDAA
                     GNRIKVKRVRLNRVRGKIQEVRLYYLESMSDPEE"
BASE COUNT         1032 a         1113 c         1110 g         1155 t
ORIGIN      
        1 agaacgcgga cgtcacaaaa caaaaagaag cagagcaaga gcgaaagaag aaagaaaaag
       61 aggcggatgc agcgcgcgaa gctggcatgc gcagacgatt tgccatcgtg ggagcctctg
      121 ccgttcgcgg attctaccag gcagcttttc aagagcagta cacgcagcac gtcctcatga
      181 cagtgcaaag aggtgggatt tttccaggag cagtctttac ttctgattta cacgaccggg
      241 acttaaaagg gggagaagca tttggatctc tcacctggaa tcgtctttat tttcgcggaa
      301 gctatctttc ttttcacgca cagccaaagt tgattcaggg gctgatgcct tcgagtcagt
      361 ctggtccggt tctgactcgt gacgatttga ctatggactt gaagagaagc gaagcgggtg
      421 cggccgcagg atattctttc gttcgcacac ccacttttga acttcgcgcc ctggctctct
      481 ttagagatga aatcatgaat gcaagcattc gctctgcggg tgcttctgtt tctaccgact
      541 ctacgagacc cttctacggc gatttcaact acgacgacgg agtgagacat cgcgcgtacg
      601 gtcacggcgg aggattgtcg gcaatttata atgttggtaa actgaaacta tcgatagatc
      661 tggaatccta tcggctccat ggcccgtggg gattgaaata taagaaaacg gaaagaagca
      721 atgggagtcc cggatttgct cctctgtacg tggatacgga aggaaccatc cgcaaaacca
      781 ccagcgtgct gtctcccgcg gcaggctatg aagtattcgc gggtttttca attcgtcttg
      841 ctgccaggtt tgagcaggcc tcttatcaga ttcgaagagc cacgatgcct cttttcctgg
      901 gcggagccag tccgccagat ttcgcaagcg cgttgtttct acagaacgta gtctacaatg
      961 gatttggctt ttcagagaaa ctaaatcggc atgatagcgt acaatatgtt tctcttggaa
     1021 tagaaaagaa aatcgatttc tagattcctg ccagttcgtc gttccagcta cttgagtcgc
     1081 gaggaaggct tcgcaaagaa tcaatcatgg cctcgcgaaa tcttcctggt gccctgcgaa
     1141 tcgtacgaat gccaccaatg caaaacaggg ccgcaatgat tgcgggcacc atccaggacg
     1201 gtcttccgaa aaagtagcgc agaatcagag tgagagtcaa cgaccccagc gccggataaa
     1261 acagcaattg ccagaatgca gcccgcaaat ggctgatgga agccgtgatc aatatgaatt
     1321 tgcccggagt gctgagtgtg atttctccat cacagttggc ccatgggttt ctgcctgcgc
     1381 cgtacatttt accccggaac gttatgcgat catctatctt ctcgcatgat tctactttgc
     1441 ggtccatatg cgccatcagt gctgactttg caggaagctt cagctctccc ggaatcgacg
     1501 tggggagaac aaccctgttt tctagaaatc tcacggttca ccgcgtgggt gtttgcccgg
     1561 gcagacaatc gtttttcgta tttttgcttt tctaatttcg ggcggtcagt tccaatgcga
     1621 gcatgcgcca tgttgctttt tgttcacttt tgattttcct ctcatgttcg ggcgcgcccg
     1681 ttcaggaggg cgccattcgc ggtagcgctg gcggttatac gatcgtattt cctcaaaagt
     1741 ggaagattgt tagaccagac gatgtgaccc ttgaggcagt gtcagaagat gaattggccg
     1801 gagttcgcat cgtagagcag ggcaggattg gaaagtcagc ggaagacatt ctgggaaaaa
     1861 tcgaaaaaca actcgaagtg cagaattcgc ttcctgaaag cagtcgcact ctggatgcaa
     1921 atcttctcaa agagagcaat gctcagcatt gtgccattgg tcagtatgca ctgagaaaga
     1981 acggagtgct ccttcgccgc aagatctggg tttttgtgaa gggtcaaagg gcttttgtga
     2041 tcaccacgaa tgttccagaa agttacgccg gagaatatcc tggtgtcttt gaaggcatca
     2101 ttaagtctct tcgcgctcag tagttcatca ggtgatgact gtaggcctct ctcttccggt
     2161 tctctctctg aggccagaga tctgagatgc aatcgtgatg aaatcgctca atgcctcctg
     2221 ggcatccaga ttgtgtctgg ccggatcggg ttcataagct tctagataga gacgcagagt
     2281 ggccccttct gttccggttc cactgagacg aaagatgatc cgcgatccgt ctacaaagat
     2341 gattctgatt ccttgacctg aactcacgct tgcatccact ggatcggtgt agctgaaatc
     2401 gtcacagaat ctgatttcat gttttccaaa tttctttcct gtaagttctg cgaagctttc
     2461 tgttacatgt ttcatcaggc cagaggccgc ctcggaagga atgccctcgt agtcatgacg
     2521 agaatagaaa ttgcgcccga atttgttcca gtgttcccgc atcacagttt ctacagactg
     2581 gtgctttact gccagaatgt tgagccagaa tagaaccgcc cacagtccgt ctttctctct
     2641 cacgtggttt gatcctgttc caaagctctc ttcgccgcat aacgttactt ttcccgcgtc
     2701 catgaggtta ccaaagaatt tccatccggt aggagtttca aaacaggcca gaccaagaga
     2761 ctgcgccact cgatccgcgg ccgcactcgt tggcatggac cgggcaattc ctgccagtcc
     2821 cttcttatat gcaggaacca gtgttgcatt cgccgcgagc accgccaggc tatcggaagg
     2881 atttacaaag aatccttttc cgagaatcat gtttctatct ccatcaccgt ccgaagccgc
     2941 ggcaaaatct ggcccgtctt ttccgtaggc aatttcaacc aggtcatggg cgtaggtgag
     3001 gttaggatcc ggatgacccc ctccaaagtc ttcgagtgga acggcattca tcaccgttcc
     3061 agccgaggct cccagtcgct tttgtagaat ttcgaccgcg tagggtcctg tgactgcatg
     3121 catggcatca aacttcattt tgaagccgct gaaaaacaat ctgcggattg catcaaaatc
     3181 aaagatggac tccatcaagg ccgcatagtc tgacaccgga tcgatcacct gcacaatcat
     3241 gtcgtctaac gtctgttccc cgacctttcc caggtttacg tctggaacct ctatccattt
     3301 gtactgcgaa atgctttttg tttttgcaaa aattgcatct gtgactttct caggcgcggg
     3361 accgccgttg gatgtgttgt atttgattcc gaaatccccg gatgggccgg caggattgtg
     3421 gctggcagaa agtatgattc cgccaaacgt gccgtgttta cgaatgacgc aggaagcggc
     3481 cggggtggat agaatgccat ccttgcccac gagtatgcgc gaaaatccgt tggccgccgc
     3541 cattttcaaa atgatctgga tggcttcgcg attgaagtat cttccgtctc ctcctacagt
     3601 gagtgcggaa ccagggggcg cgcctatgct atcgaatatg gattgaacaa agttctgtag
     3661 atagtgttcc tgtttgaaca cgggcacttt ctttctgagt ccagaggtcc cgggcttttg
     3721 atctgcaaaa ggctttgtat ctatgatccg aatgttcatt tccgtctccg cggttgagtc
     3781 ttcagggcat ttgcggaact gtcaaggcta gaacccgcgg cgaatcattc ttccgggtca
     3841 gacatggatt ccagatagta gagacgcacc tcttgaatct tgccgcgcac tcgattgagt
     3901 ctcactctct ttaccttgat tctgtttcct gcggcgtctc tggtgactcc atcgatcagt
     3961 atgtccacgc cgctcagctt ggtaagagac tccagacgac ttgccagatt gactgcatcg
     4021 cccagaaccg tgtattccat tctacgaact gaacctgtat tgccagcaaa cacgttccct
     4081 gatgcgattc ctattccgat tcgcactggc tctgtcagat agtccggtcg cacgtcgttg
     4141 aatgtgttca tgtattcgcg cacttgaagc gcaaaccgca gtgcattgcc cttgtcatct
     4201 tctgtttgaa tggggcagcc aaacgttgcg agcattccgt ccccaatgag cttgttgacc
     4261 gatccatgat ttccgtacac cagatccatg atatccgtga agatcgagct gataaaatct
     4321 gaaaattgtt gcggatcgat tttctcagcg attgccgttg aattgcggat atccagaaag
     4381 acgattgtgg ccggaacgcg cgcgcctccc
//
LOCUS       DAORFF010000321         4403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11603_length_4403_cov_3.285419, whole genome shotgun sequence.
ACCESSION   DAORFF010000321 DAORFF010000000
VERSION     DAORFF010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11603_length_4403_cov_3.285419"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PKK76_12645"
     CDS             complement(<1..888)
                     /locus_tag="PKK76_12645"
                     /inference="COORDINATES: protein motif:HMM:NF019316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNL69373.1"
                     /translation="MRGIVTLILCLFVTPIRAQTPIDIGDGMPLLSLAAISETHAGSP
                     LDSIDSVSQQDDWKGPGEEFRFGLRGEPVWIHFRVRNPSADPATAFVSIPAPWISHAQ
                     LFRSTEGAWVKTETGYAVPHAQWDVRSFYPSLRILLGSRENADLYLRIESNRVLNFPA
                     SIMDRSEFRQRDRARTTISGLMIGVFLALLSLACILLFVERNSAFTLYCLYLLFLAAG
                     LAAMMGNGIQFFASVEPQILSRIGIMAFPAARAILLLMIPFFCGRPIAGNQIIIRIAG
                     IEMICGLVLAAGGWTISAQI"
     gene            complement(1257..4061)
                     /locus_tag="PKK76_12650"
     CDS             complement(1257..4061)
                     /locus_tag="PKK76_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNL69374.1"
                     /translation="MKDFPEGRTLERTLLQNLGEGVYGVDQDGLCTFVNEAALAMLGF
                     SEAEVLKKDQHTLFHWRKEDGSPYPPEECPVSKTIRDLQTRHVEDSFVCKDGRMLPVE
                     LTIAPVSDEAGRPGAVVAFQDLTELRKARAEAKEAVEMRQVLLDQSLAGIALLKDRRI
                     LWANRRMETMFGYNTGGLHGLETSALYAANADFQTLGEQAYPTMQAGGTYSDELLMKR
                     KDGATFWCQLSGQLVNPREPARGSIWTISDVDENHRRAQELVESEERYRQAFEVNSAI
                     KLIIDEETGAIVDANGAACHFYGYSREQLLRMNIRDINCMSPDEITRELTAAGKQQRL
                     VFEFKHMLSSGEIRDVEVYSGPIRVDGRPRLHSIIHDITERKKAQSDLELAARVITGA
                     AEGVVVTDQHAKILAVNPAFTEITGFAQSEAIGQTPRILASGRQSQDFYQAMWEDLIA
                     KGNWAGEIWNRKKDGTVYPEWLSISRTGSGADLRYIAIFSDITRRKADEERIRRLAHY
                     DPLTGLPNRALLFDRAMQALSLCRRGDMPLALMFLDVDHFKRINDTLGHKIGDEILVA
                     LSQRLTGILRVEDTVCRLGGDEFVILLPEIGAEGAAHVATKLVESIQTPLNVSGGIAV
                     TLSVGITMFPEDGDDFEILCKNADTAMYRAKAEGRNGFRFYTAEMQKKAARHWELEHA
                     LRSALANGELAVHYQPQLSLDSSTVTGVEALLRWTHPQLGAVSPVEFIPIAEEIGLIT
                     TIGHWVLRCAVKQAVSWIQSGRESLVMSVNVSAAQLREAGLVDQILDILKEEGLPAKN
                     LELEITESLAMQDPALAIRMIRILRDAGISVAVDDFGTGHSSLSSLKRFNFSRLKIDR
                     SFIQEIAVEPESRAIVRAILGISEALGLATVAEGVENAEQLDTLRELGCEDSQGFLLA
                     IPMPAEDATRWMESHAPMNR"
     gene            complement(4058..>4403)
                     /locus_tag="PKK76_12655"
     CDS             complement(4058..>4403)
                     /locus_tag="PKK76_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69375.1"
                     /translation="KVIATPVTEIFSESPVYGYDNEKTVVDIVAVNQIKRLNQKYEKV
                     RNGLLHDLLRRLARFRVTKKQTNYRRGISSESILILNSGHALSHTDARKSFALALFPS
                     IRNHGRKMVRTR"
BASE COUNT          989 a         1167 c         1157 g         1090 t
ORIGIN      
        1 aatctgagcg ctgatggtcc agccacctgc agcgagaacc aggccgcaaa tcatttcaat
       61 cccggcaatt cggatgatga tctgatttcc tgcaatcggc ctgccacaga agaatggaat
      121 catgagcagc aggatggctc gcgcggctgg aaaagccatg atgccgatgc gcgaaagtat
      181 ctgcggttct acggaagcaa aaaactgaat tccgtttccc atcatagccg cgagtcctgc
      241 cgccaggaag agaagataga gacaatagag tgtgaaggca ctgttgcgct cgacaaaaag
      301 caggatgcac gcgagggaaa gcagcgcaag gaatacaccg atcatcagtc cgctgattgt
      361 cgtccgggca cgatcacgct gacgaaattc tgaacgatcc atgatggagg ctggaaaatt
      421 cagaactcga ttggattcga tgcgcagata gagatctgca ttctctcttg atcccagcaa
      481 aatgcgaagg gaagggtaga agctgcgcac gtcccattgc gcgtgcggga cggcataacc
      541 tgtttctgtt tttacccaag ccccttcagt cgaacgaaag agctgggcgt gactgatcca
      601 gggtgcgggt atgctaacaa aggcagtagc tggatctgcg gaagggttgc gaacacgaaa
      661 gtgaatccag accggctcgc cccgcaatcc aaaacggaat tcctcaccgg gtcccttcca
      721 gtcgtcttgc tgagaaaccg aatcgatcga gtccagtggc gatccagcgt gtgtttccga
      781 aatcgcggcc agagaaagaa gtggcatgcc gtccccaatg tctattggag tctgtgcgcg
      841 gatgggcgtg acgaacaggc acagaatgag cgtcactatc ccgcgcataa agcaccatct
      901 taaggcgccg atcctggttt tgcaactccc taacaaatgt gagatcgagt attaagttcc
      961 tctattttcc agatccgagc agaagcgcgg ctgatctttc ttgcgcgcat ccgttggtcg
     1021 ctggtttttt cctccggcca cgcgggctgc tcccttcttt ctcattcgtt ttgtgtgcaa
     1081 tcaatccgcg atccaaggat tgggtttgtc gctgggcgtg aacgtggctg cgtccgtatc
     1141 ttctggcgcg gcattccagg catgcccatg caaaaagaat cggaggattg ccggcccttg
     1201 tagacggtgt gtgccggttc gacacttcga ggaaaaccca gacagaataa tgtcgcctaa
     1261 cggttcatag gtgcgtggga ttccatccag cgcgttgcat cttcggcagg cattgggatg
     1321 gccaggagaa agccttgaga atcttcgcaa ccaagttcac gaagcgtatc aagttgctcc
     1381 gcattttcga cgccctctgc gactgtggcc aggccaaggg cttctgagat tccgaggatg
     1441 gcccgaacaa tggcgcgact ttctggctct actgcaattt cctgaatgaa ggagcggtca
     1501 attttcagac gtgagaaatt gaaacgtttg agagaggaga gcgacgagtg acccgtccca
     1561 aaatcgtcca cggcaacact gatgcctgcg tcccgcaaaa tcctgatcat tcgaatggcg
     1621 agcgctggat cttgcatagc gaggctctcc gtgatctcca gttccaaatt ttttgccggc
     1681 aagccttctt ctttgaggat gtctagaatc tgatccacaa gcccggcttc tcgcagctgg
     1741 gcagcagaga cgttaaccga cattactaga ctctctctac cgctctgtat ccaggagact
     1801 gcctgtttca ccgcgcagcg cagtacccaa tggccaatcg tcgtaatcag gccgatttcc
     1861 tccgcgattg gaatgaactc gaccggagag actgcaccca gctgtggatg cgtccagcgc
     1921 aagagcgctt ccactcccgt gaccgtgctg gaatccaggg ataactgcgg ctggtagtga
     1981 actgcgagtt ccccattcgc cagtgcgctg cgaagagcat gctcgagctc ccagtgtcgc
     2041 gccgctttct tctgcatttc agcagtgtag aagcgaaaac catttcgtcc ttctgctttc
     2101 gcgcgataca tggccgtgtc tgcgttcttg cagagaattt caaaatcatc gccgtcttcg
     2161 ggaaacatag tgatgccaac agaaagagtc actgcaattc cgccggaaac gttaagcggc
     2221 gtttggatgc tctcaacgag cttggtcgct acgtgcgctg ctccttctgc tccaatttct
     2281 gggaggagaa tgacaaattc atctcctccc agtctgcaga cggtgtcctc cactcgcagt
     2341 atgccagtga gtctctggct tagagcaacg agaatctcgt caccgatctt atgccccagc
     2401 gtatcgttga tccttttgaa gtgatccaca tcaagaaaca taagtgccag gggcatatct
     2461 ccgcgccggc acaacgagag tgcctgcatt gcacgatcaa atagcagcgc acggttgggt
     2521 aaaccagtaa gaggatcgta atgtgccagt cgtcgaatgc gttcttcatc cgcttttcgt
     2581 cgcgtgatgt cagaaaagat ggcgatgtac ctcaggtctg cgcctgagcc agtgcgactg
     2641 atggacaacc actcggggta aacggtaccg tctttcttgc gattccagat ttcaccggcc
     2701 caatttcctt tcgcgatcaa atcttcccac attgcctgat agaaatcctg gctctgtctg
     2761 cccgaagcga gtattctggg tgtctggccg attgcttcgg attgtgcgaa accggtgatc
     2821 tcggtgaacg cgggattgac cgcgagaatt ttggcatgct ggtctgtaac tacaacgcct
     2881 tccgccgctc ctgtgatgac gcgcgctgcc agttccaggt cagactgagc tttctttcgc
     2941 tctgtgatgt cgtgaatgat agagtggagg cgaggccgtc catccacgcg gattggaccc
     3001 gaatacactt ccacatcccg gatttcacca gatgacagca tgtgtttgaa ctcgaagacg
     3061 agtctctgtt gttttcctgc tgcagtcaat tctcgcgtga tttcgtcggg ggacatgcaa
     3121 ttgatatcgc gaatgttcat gcgcagcaat tgttcccgtg agtagccata gaagtggcat
     3181 gctgctccgt tcgcgtcgac gatcgccccg gtttcctcgt ctatgatgag tttgatggca
     3241 ctgttgactt cgaatgcttg ccgatagcgt tcttcgcttt cgacaagttc ctgggcgcga
     3301 cggtgatttt catcgacgtc tgagatagtc cagatagatc cacgcgctgg ttcccgggga
     3361 ttgacgagtt gtccgctcaa ctggcaccag aaggtcgcac cgtctttgcg tttcatgagg
     3421 agttcatctg aataggttcc tcccgcttgc atcgtaggat atgcctgctc tccgagtgtc
     3481 tgaaagtcag cgtttgcagc atagagagcg gaggtttcaa gtccgtgaag cccaccagtg
     3541 ttgtacccaa acattgtttc cattcggcga tttgcccaga ggatgcgacg atcttttaga
     3601 agcgcgatgc ccgcgagact ctgatcgaga agtacctgtc gcatttccac agcttctttt
     3661 gcttccgctc tcgctttgcg caactctgtc aaatcctgga acgcaaccac ggcgcccggg
     3721 cggcctgctt cgtctgaaac cggagcaatc gtaagttcga ccggaagcat ccgaccatcc
     3781 ttgcacacaa aggaatcttc tacgtgccga gtttgcaaat cgcgaatggt tttggacacc
     3841 gggcattcct ccggtgggta gggacttccg tcttcttttc gccaatgaaa caaggtgtgt
     3901 tgatcctttt tcagcacttc agcttcagag aagcccagca tcgcgagtgc tgcttcattc
     3961 acaaatgtgc acagtccgtc ttgatctaca ccatacacgc cttcgccgag gttctggaga
     4021 agggttcgtt ccagcgtgcg accttcaggg aaatccttca tcgggttctg accatttttc
     4081 ggccatggtt gcgaatgctc ggaaacaatg ctagcgcgaa tgactttcgc gcatccgtgt
     4141 gcgaaagagc gtgtcctgaa ttgagaatca agatcgattc cgaagagatt cctctcctgt
     4201 aattcgtttg ttttttcgtg acgcgaaagc gtgctaaacg tctgagcaaa tcatgaagca
     4261 agccgttgcg tactttttcg tatttttgat tcaatctttt gatctggttg actgctacga
     4321 tatcaacaac cgtcttttca ttgtcgtatc catagacagg actttcagaa aaaatttctg
     4381 tgacaggtgt cgcgataacc ttt
//
LOCUS       DAORFF010000322         4379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11711_length_4379_cov_3.331406, whole genome shotgun sequence.
ACCESSION   DAORFF010000322 DAORFF010000000
VERSION     DAORFF010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11711_length_4379_cov_3.331406"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /locus_tag="PKK76_12660"
     CDS             <1..1733
                     /locus_tag="PKK76_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69376.1"
                     /translation="ETLHGKWLRYDTNLEPLDWSTHPEIPEIRAFALQASKRFLDFWN
                     EVFDVAVARALATRDRGILRHKLFEPSSYFDIGVPEEQRHSSIPPALHSKIISEYNAS
                     RFAETENHVYYLTEYLSLLSDDWIEKLKPFEKLWNLTDEKARLTQEISELRATTEEEQ
                     NLLQAANSLFFHASRAHRIRLRLILLRGQKRSASQLLDALSRSQTEIDSRYAILNEKP
                     IESKDTFAGSGLSADSYLSLCRSILDTNSAIESIYLKDVDPALERSMVREACRDILHR
                     LGPIRRAAAGYSLIAGERHGAMSLNPVMRLWKEKPNPPRSEAPALSLEMNTKQTIDCL
                     IRLAHLADPDLGHRELMRGKIAKLMRGIENLANLKNFLIPGSAYPLREIHPLDYPEFR
                     WRVIGETRSPLELGANETAVLTGAWYSKRQHSLYCTIGADNGRLLRILWNSERSPGPP
                     AFYFAMGQFVHDCMEDSLIYRRSGSITFRECVEDYYDFEDRIRKNKGEKTGRRKSDNS
                     RPGVRFMFAVLYSRMITELLTGSLQSQFRHPATEKWMETNLGLSRDRARLKHIRIKLR
                     EIISNLRVHA"
     gene            1767..2106
                     /gene="rnpB"
                     /locus_tag="PKK76_12665"
     ncRNA           1767..2106
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKK76_12665"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2197..4017
                     /gene="uvrC"
                     /locus_tag="PKK76_12670"
     CDS             2197..4017
                     /gene="uvrC"
                     /locus_tag="PKK76_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNL69377.1"
                     /translation="METGAEIVQAVQRAPTAPGCYIWKDSSGEILYVGKAISLRARLR
                     NYLNPQDTKTQFLMDRAATLEWVTTETNREALILEATLVKKHQPRFNVRLKDDKRYPW
                     ICISMSEMYPQIFITRTVRDDGNLYFGPYTDVGATRNTLSLILKIFPIRKVRQKLPLP
                     KPKRPCMNFHIGRCLGPCQGNVDPEEYARIVEQVRLFLEGRTEILETLVGKRMEEASE
                     RMDYEKAAMFRDVLANIRRATERQIISDPGAADEDIVAFAARDDDGQMVVFEMREGKL
                     TGRKSFPLKGVLNSDPEEIMTSFVRDCYLNASIRPATIILPGKIKAKRELEEMLSENG
                     RSIRITTARKEEAELLSRMAQKNAELLLRERLLAARMKDRSRGLEEIKEMFSLPEVPS
                     VMECYDISHLQGSHTVASGVFFVDGEPSRKDYRHYKIKTVPGIDDPASMEEVISRRLQ
                     RLLAEDRALPDLILIDGGTTQLAAACRAANALGAGDVPIIGIAKQREEIYFPGEPTPR
                     HFDKDSAGMLILRHMRDEAHRFGITFHRSRRNQAVMRHMVEEIPDIGVSRLRSLLKHF
                     AEHSIEEATVEELMKVPGIGKALAGKIHGYFQNKTGAKTE"
     gene            4054..>4379
                     /locus_tag="PKK76_12675"
     CDS             4054..>4379
                     /locus_tag="PKK76_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL69378.1"
                     /translation="MKILTVDDSSTVRFVMKSAIESLGHTCLMATNGQEALAIVSAEP
                     IDVVLLDWNMPLMDGREVLREIRKKPICKNTKVLMCTSEATRGDVEQILRLGVEGYIL
                     KPISPD"
BASE COUNT         1199 a         1094 c         1119 g          967 t
ORIGIN      
        1 cagaaactct tcacggcaaa tggctgagat acgacacaaa cctggaacct ctggactggt
       61 ccactcaccc tgaaattcct gagattcgag cattcgctct gcaagcttct aagcgatttc
      121 tggatttctg gaatgaagtc tttgatgtag cggtcgcacg ggctctggca acgcgggatc
      181 gcggaattct cagacacaaa ctttttgaac cgtcctctta ttttgacatc ggtgttccag
      241 aagagcagcg tcacagctcg attcctcctg cgcttcattc caaaatcatt tcagaataca
      301 atgcatcgcg atttgcagaa actgaaaatc atgtgtacta cctgacagag tacctgagcc
      361 ttctttcaga tgattggatc gagaagctca aacctttcga aaagctatgg aatctaacgg
      421 atgagaaggc ccgactgacc caggagattt cggaacttcg ggccacaaca gaggaagagc
      481 agaacctttt gcaggccgcg aattccttgt tttttcacgc atcccgcgcc cacagaatca
      541 ggctcagact gatcttgctt cgcggtcaaa aacgatccgc ttcacagcta ctcgatgcac
      601 tctctcgttc gcagaccgaa atcgattcac gttatgcgat cttgaacgaa aaaccgatcg
      661 agtcaaaaga caccttcgcg ggttccggac tttccgccga ttcctatctt tctttgtgta
      721 gatcgattct cgacacgaac tctgcgatcg aatccatcta cttgaaagat gtggatcctg
      781 ccctggagcg aagcatggtg cgcgaagcat gccgggatat ccttcatcgc ctggggccga
      841 ttcgtcgcgc ggccgctgga tactcgctga ttgcaggcga aagacacgga gccatgagcc
      901 tcaatccagt gatgcgtctg tggaaagaga aaccaaatcc tcctcgaagt gaagcccctg
      961 ccctttcgct tgagatgaat acaaaacaaa cgatcgattg tctgatcagg ctggcccatc
     1021 tcgccgatcc ggacctggga cacagagaat tgatgcgtgg aaaaatcgca aagcttatgc
     1081 gcggaattga aaatctggcc aatctgaaaa acttcctgat tcctggatcg gcctaccctt
     1141 tgcgtgaaat ccatcctctg gactaccctg agttcagatg gagagtcatt ggtgaaacgc
     1201 gaagccctct ggagctgggc gcaaatgaaa ccgcagtgct gacaggtgca tggtacagca
     1261 agagacaaca cagcctctat tgcacaattg gagcggacaa cggcagactc cttcgcatcc
     1321 tctggaattc ggaaagatct cctggaccac ctgcttttta tttcgccatg ggacaattcg
     1381 ttcacgattg tatggaagat tctctgattt atagaagaag cggaagcatt acgtttagag
     1441 agtgcgtgga agactactat gactttgaag atcgaatccg caaaaacaag ggagagaaaa
     1501 ctggccgcag aaaatctgat aactcaaggc ctggcgttcg attcatgttt gccgtgcttt
     1561 attcccgcat gatcactgaa ctactcacgg gttcgctaca gagtcagttt cgtcatcctg
     1621 ccacggagaa atggatggag acaaatctgg gactgagtcg tgaccgggcc aggctcaagc
     1681 acatccgaat caagttgagg gaaatcattt cgaatcttcg cgttcacgct tgactttgaa
     1741 atcagattta gcatcctacc tgtgaggggg tgcaccggat cgccgcgcga acctgctttg
     1801 cagggagtga ggaaagtccg gacaccacag gacaggagac cgggtaacgc ccgggcattg
     1861 tcgtctaacg gcagtgacgg aaagtgcaac agaaagatac cgcccttcgg ggtaagggtg
     1921 aaatggcggg gtaagagccc accgcgcaaa tcgtaagatt tgtggtacgg caaaccctct
     1981 ccggtgcaat ctcgcatagg cagtcagagg tcggtccgac cgggactgcg ggtagagagc
     2041 aaagaagctt gtctcaagat aaatggcgat ccacgacaga atccggctta caggtgcact
     2101 ccctcacctt ccgggcgatt cggccgcttt tcttgaagtg tgacacgagc actgaatgat
     2161 gctgacagtg ggcctgcgaa tcgctttttt gactcagtgg aaacgggagc tgaaatcgtc
     2221 caggcagtgc agcgggcgcc aacggctcct ggctgttata tctggaaaga ctcttccggc
     2281 gaaattctgt acgttggcaa agcaatcagt cttcgcgcaa gacttcgcaa ctacctgaat
     2341 ccacaggata ccaaaacgca attcttgatg gatcgcgcgg ccactctcga gtgggtcacg
     2401 acggagacca accgcgaagc actgatcctc gaagccactc tggtaaagaa acatcaacct
     2461 cgattcaacg ttcgcctgaa ggatgacaag cgatatccat ggatctgcat ctccatgtct
     2521 gaaatgtatc cgcagatatt catcacgcgc acggtgcgcg acgatggaaa tctgtacttt
     2581 ggaccttata cagatgtggg tgcgactcga aacactctct ctttgattct aaagatcttc
     2641 ccaattcgca aagtaagaca gaagcttcct cttccaaaac caaaaagacc atgcatgaac
     2701 tttcacattg gtcggtgtct tgggccgtgc cagggaaatg tggatccaga agaatatgcg
     2761 cgcatcgtag aacaggttcg tctcttttta gaaggtcgta ctgaaattct ggaaacgcta
     2821 gttggcaaga gaatggaaga agcaagcgaa agaatggact acgagaaggc agccatgttt
     2881 cgcgatgttc ttgctaatat aaggcgagcc acagaacgcc agatcatttc tgacccggga
     2941 gcggcagacg aagacattgt ggcgtttgcg gcgcgcgatg acgacggaca aatggttgta
     3001 tttgaaatgc gcgaaggaaa gctcacaggt agaaaatcat ttccactcaa gggagtgctc
     3061 aactcggatc cagaggagat catgaccagc tttgtgcgcg actgctatct gaacgcatcg
     3121 attcgaccag ccacgatcat actgcccggc aaaatcaaag caaagagaga actggaggaa
     3181 atgctttccg agaacggaag gtctattcga atcacaaccg ctcgcaaaga agaagcggag
     3241 ttgctcagtc gcatggcgca aaagaacgca gaactactgt tacgcgaaag acttctggca
     3301 gcgcgcatga aagaccgatc tcgcggcctg gaagaaatca aagaaatgtt ttcccttccg
     3361 gaagttccgt ccgtcatgga atgctatgac atatcccatc tgcagggtag ccatactgtc
     3421 gcctctggag tgttttttgt ggatggcgaa ccttctcgaa aagactacag acattacaaa
     3481 atcaaaactg ttccaggcat agacgatcct gcttccatgg aagaggtcat atccaggcga
     3541 ttgcagagac tgcttgcaga agacagggca cttcctgatc ttatcttgat agacggtgga
     3601 acgactcaac tcgcagccgc gtgcagagcc gcaaacgcgc ttggggccgg ggatgttccg
     3661 atcatcggaa ttgcaaagca aagagaggaa atctatttcc ctggagaacc cactccgcgt
     3721 cacttcgaca aagacagtgc aggcatgctg atcttgcgtc atatgcgcga tgaagcacac
     3781 agattcggca tcacatttca tcgctccaga aggaatcagg ctgtgatgcg tcacatggtg
     3841 gaagaaattc cagacatagg agtgtccagg cttcgctcgc tcctgaagca tttcgccgaa
     3901 cattcgatcg aagaagcaac tgtggaggag ttgatgaagg tgcccggaat cggtaaagca
     3961 ctcgcaggta aaatccacgg atactttcag aacaaaacag gcgcgaaaac agaataagct
     4021 tgcgaaaaca aagcacggag gtctaatctt agcatgaaga tactcaccgt agatgattca
     4081 tccacagtgc gctttgtaat gaagtctgcc atcgaatccc tgggccacac ttgcctcatg
     4141 gccacgaatg gtcaggaagc actcgccatc gtttccgctg agccaatcga tgtagtgctc
     4201 ctggattgga atatgcctct catggacggc cgcgaagtac tgcgcgaaat tagaaagaag
     4261 ccaatctgca agaacacaaa ggttctcatg tgcacgtcag aagcgactcg aggagatgta
     4321 gagcagatcc ttcgcctggg agtagaaggc tacatactta agcccatttc tccggacaa
//
LOCUS       DAORFF010000323         4362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11784_length_4362_cov_4.031344, whole genome shotgun sequence.
ACCESSION   DAORFF010000323 DAORFF010000000
VERSION     DAORFF010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11784_length_4362_cov_4.031344"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            655..2742
                     /locus_tag="PKK76_12680"
     CDS             655..2742
                     /locus_tag="PKK76_12680"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL69379.1"
                     /translation="MLAIFPGASYAYSKVEDLPENGNKTGLVREGSTTDLAPSHNSGK
                     LTSANGGGRAPSYLSPGRIYRDMGLRYKMIVLILGVVSVSVIPLSLIVLYRNQAVVLD
                     KTYEVCLSVTNHISGLATEELLVNETYDATQTAFARLRQSKVTGLEDAYIINIDGEYV
                     AEYNNAGRIHQKARQETVQLMRSLASPKTETTTINGKPTILFSFPILIRSKSQTLRVG
                     AAFFEFNRDEVYAPVNQIRNTILVVAGILFVFGIAMAYYVAYFFSRPIQKLSEGARLI
                     GEGDLNHRIRLYGKDELGRLATTFNQMTQKIQDFTHNLEAMVDQRTRELNETLAQVQA
                     LKIQQDGDYFLTAILVEPLQPNNNKCDNVRTEFRIEQKKKFHFRKWNSMIGGDVCITD
                     TITLMDREYAVFINGDAMGKSLQGAGGVLVLGAVFNSSLVRTHLQKNLKIYPEGWLRE
                     RFMELQAVFTSFNGSMYISVCMGLVDNLTGMLYYINAEHPFTVLYRKGKATFLEQELE
                     LRKLGMPEQEEQFRVRTMQLLAEDVLIAGSDGRDDIVIRNPQTGEEKVNDDEFLFLKH
                     VEQADGLLDGIIDRLKASGTVLDDISLLRLSYRENNPAPIAHIPVEIRETIADAQRLV
                     EAGRHEEVLARLEDLFAANRSLPELLKTLGAIYFHKQEYARAAECFESYLEEVPSDNE
                     YIYALSNVYRLGG"
     gene            2845..3345
                     /locus_tag="PKK76_12685"
     CDS             2845..3345
                     /locus_tag="PKK76_12685"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL69380.1"
                     /translation="MARALFMVTRALEADADNSQAMALEARIREAQSADPTLSGEGET
                     SARMAEAEELYAKKKYKQAIDLFLDVARMDARQNRALFRAANCYVFLNRLESAAHYYR
                     KSLAVHAEDVHVLNNLGSVYFRLKKYDEARESWNEAVRLQPGFQPARVNLERLDRFTS
                     QMETPV"
     gene            3342..>4362
                     /locus_tag="PKK76_12690"
     CDS             3342..>4362
                     /locus_tag="PKK76_12690"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNL69381.1"
                     /translation="MKACVFFVSLLLSLSGSLRAQAGTIPVILSSESTIYESVLSGIG
                     SAMNRRLTVQYLDAIIARYPDPAAYFQALEAQKPELVITIGSAASTLARDNLKQTPIV
                     FSMVNTPRLLGVGSRNICGVGMGISIGEFFRSIRDIAPTARFVHAFYTTDAGETEAME
                     GDYLDYQNKLVFVRHRLSGPAELGSALEKIKGHSDAILIVNDPLYSRTAFEKVSSFSS
                     QNHMILAAPFPALVKLGTTFGISPDYSKIGVQTGEMALRVLSGKSTCYAELVQPPEHN
                     AFYLNEDYAAKSGIAIPPKIVARAKLTQQFALGVDLLNQGKLKSARLVFENILKAATV
                     KCTAQY"
BASE COUNT         1094 a         1058 c         1131 g         1079 t
ORIGIN      
        1 cctgcaatga gtgcctggat ctctttctcg aattttgatt ccgctgataa gcgcactgca
       61 tccatctata aactctccgc tgggcaatcg gcatatagat gaggcgaagt ggcaattcaa
      121 aaatcggcgt ttctggttcc ggctggcttc cgggctctgg aagtcaggat cgtttttgcg
      181 gtgcgaatct ggaatcaacg acacgaaatt ggtaatgaac aacactttca aacgttaagc
      241 gatctttctt gaaattgttt tatgttttta tatagtttag atgtggttat tcaatgaaaa
      301 tgcattgacc cgttcttgcg gcgttccggt tctggagatg tactgattgc tgattcgcgg
      361 ggcgtcagaa ggagtttaca atgttcaaac gcttcctgat ctctctttcg gttttgctcg
      421 ttgtgttttg cgcggaaaaa aaggaggagg ataattccag cctgcttcta ctcgggttgc
      481 taggggccgg aagggcaagt tctacgagct cttcggcgac ttgcggtggt gttgccctca
      541 cttccgccac tgcgttgaca gacgatgctg ccgcagtgac aagcagtaca cttcccggaa
      601 tctacgccgc ctaacgttgg acgttttgaa cgaaaagtca caaatttttg aagagtgctt
      661 gctatttttc ccggtgcgag ttatgcctac tcaaaagtgg aagatctacc agaaaacggg
      721 aacaagacgg gcttggtgcg cgaaggctcc acgacggatc tggctccttc gcataactct
      781 ggtaagctga cctcggcaaa tgggggtggg cgcgcccctt cgtatctttc ccccggtcgc
      841 atctatcgcg acatgggttt gcggtacaaa atgatcgtct tgatcctggg ggtggtatcc
      901 gtgagcgtca ttccgctttc actgattgtg ctctatcgca accaggcggt ggtcctggac
      961 aagacgtatg aggtctgtct cagcgtaacc aatcacatat caggtctcgc aaccgaagag
     1021 cttcttgtaa atgaaactta tgatgcaacg caaacagcat ttgcgaggct caggcaatcg
     1081 aaagtaacag gtctcgaaga cgcttatatc atcaacattg acggagagta cgtagcggaa
     1141 tacaacaacg ccggaagaat tcatcaaaaa gccaggcagg agacggtgca actcatgcga
     1201 agtctcgctt cgccaaagac agaaacaacc accatcaacg ggaagcccac gattcttttc
     1261 agcttcccca ttctgattcg aagcaagagt cagacactgc gcgttggggc tgcgtttttc
     1321 gaattcaatc gcgatgaagt gtacgcaccg gtaaaccaga ttcgtaacac gattctggtc
     1381 gtggccggca ttctctttgt gtttggcatt gctatggctt actacgtcgc ctacttcttc
     1441 agtcggccca ttcagaagct ctcagagggc gcgcgattga ttggggaggg ggatctgaat
     1501 catcggattc gcctgtatgg gaaggatgag ctgggtcgcc tggcaaccac gttcaaccag
     1561 atgacccaaa agattcagga ttttactcac aacctggagg caatggtaga ccagcgcacg
     1621 cgtgaactca acgaaactct ggctcaagtg caggctttga agattcagca ggacggggat
     1681 tatttcctga ctgcaattct cgtggagccg ttgcaaccca ataacaataa gtgtgacaac
     1741 gtccgtacgg agtttcgcat cgagcaaaag aagaaattcc attttcgcaa atggaactcc
     1801 atgattggag gtgatgtctg catcacggat accatcacgc ttatggaccg cgaatacgcc
     1861 gttttcatca acggagacgc gatgggaaag tctctgcagg gagccggcgg cgttctggtt
     1921 ttgggggcgg tattcaactc aagcctggtt cgcactcatc tccagaaaaa cctcaagatc
     1981 tatcctgaag gctggcttcg tgagcggttc atggaactac aggccgtatt cacgtcgttt
     2041 aacggtagca tgtacatatc cgtgtgcatg ggtcttgtag acaatttgac cggaatgctc
     2101 tactacatca acgcagagca tcctttcacg gtactgtatc gcaaagggaa ggcaacgttt
     2161 ctcgagcagg agctcgaact gcgaaagctg ggtatgccag aacaggaaga acagtttcgc
     2221 gttcgaacca tgcaacttct ggcagaagac gtattgatcg ccggatcgga cggacgtgat
     2281 gacatcgtca ttcgaaaccc acagacaggc gaagagaaag taaacgatga tgaatttctc
     2341 tttctcaagc acgtagaaca ggcagatggt ttgctcgatg ggattattga caggctcaaa
     2401 gcaagcggca ccgttctgga cgatatttct cttctcaggc tttcttaccg cgaaaacaat
     2461 cccgccccga ttgctcacat tcctgtcgaa atcagagaaa caatcgcaga tgcgcagaga
     2521 ttggtggaag ccggaaggca tgaggaagta ctggcgcgtc tcgaagatct ctttgcagcc
     2581 aatcgatctt tgcccgagct cttgaaaact ctgggcgcaa tctattttca caaacaagag
     2641 tacgcgagag ccgctgaatg ctttgaaagt tatctcgaag aagttccttc ggacaacgaa
     2701 tacatctatg cactctcaaa cgtgtacaga ctgggcgggt aactgaatcg ctccgcggac
     2761 ctcggtgaac gtctctttct tcgcgacaga aaacacctac tcaatctcct gaatctgagc
     2821 gccgtctatt tcgaaatggg agtgatggct cgcgcgctct ttatggtcac gcgagcactc
     2881 gaagcggacg cagacaactc gcaggcaatg gcactcgagg ccaggattcg cgaggcacaa
     2941 tctgcggacc ccactctgtc aggcgaagga gaaacatcgg cccgcatggc agaggcagaa
     3001 gaactctacg ctaagaagaa atacaagcag gccattgatt tatttctgga tgtcgcgcgt
     3061 atggatgcga gacaaaatcg cgcactcttt cgtgcagcaa attgctatgt gtttcttaat
     3121 cgtttggaga gcgcggcaca ctactatcgg aagagcctgg cagtccatgc agaggacgtc
     3181 cacgttttga ataatctcgg ttctgtgtat tttagactca aaaaatatga tgaggcacgt
     3241 gaatcatgga atgaggcagt gcgtttgcag ccaggatttc aaccagcacg cgtaaacctg
     3301 gagcggctgg accgtttcac ttcacagatg gagacacccg tatgaaagca tgtgtctttt
     3361 ttgtaagtct tctgttgagt ctttctggct ctctacgagc tcaagcgggt accattcctg
     3421 taattctgtc atcagaaagc acgatttatg aaagcgtgct ttctgggatt ggttccgcga
     3481 tgaatcgtcg cctaacggtg caatatttgg acgcaatcat cgccagatat ccagatcccg
     3541 ccgcttactt ccaggcgctc gaagcgcaaa aaccagagct tgtaattacc attggttctg
     3601 ccgccagtac tcttgcgcgc gacaatctaa agcagacgcc cattgtattt tctatggtca
     3661 atacgccgag gctgcttggc gtcgggtcgc gcaacatttg cggagtgggg atgggcattt
     3721 ccatcggcga gttctttcgc tccatcaggg atatcgctcc cacggcacga tttgttcacg
     3781 ccttctacac cacggatgcc ggtgaaacag aggcgatgga aggggactac ctggactatc
     3841 agaataaact ggtgtttgtt cgccatagat tgtcgggccc ggcagagctt ggaagcgcgc
     3901 ttgagaaaat caaagggcat tcggacgcca ttttgattgt gaatgatcct ttgtattcgc
     3961 gcacagcgtt tgaaaaagta tccagcttct ccagccagaa tcatatgatt ctcgccgcac
     4021 cgtttcctgc ccttgtgaaa ctcggaacta cctttggaat cagcccggac tacagcaaga
     4081 tcggagtgca gaccggagag atggctctgc gtgttttgtc tgggaagtcc acctgttatg
     4141 cggagctggt gcaacctcca gagcacaatg cgttttactt gaatgaggat tatgccgcga
     4201 aatctggaat tgcgattccg cccaagattg tggcaagagc taaactaacg caacaattcg
     4261 ctctgggcgt ggatctactg aaccagggaa agctgaagtc tgctcgactg gtttttgaaa
     4321 acatactcaa ggctgccaca gtcaaatgta ctgcacaata tt
//
LOCUS       DAORFF010000324         4358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11808_length_4358_cov_3.011155, whole genome shotgun sequence.
ACCESSION   DAORFF010000324 DAORFF010000000
VERSION     DAORFF010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11808_length_4358_cov_3.011155"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKK76_12695"
     CDS             <1..431
                     /locus_tag="PKK76_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69382.1"
                     /translation="ISAVLLAYAVLWPDRKALFFFVIPMRMRWVILVFAVLIAVSSMV
                     ALLQVLGGALGGALFIYYYLRKGNRTYGQSVEDRTMTLSERWEEFKKKRRLNKKKAEI
                     ERRIEMKEEVDRLLEKISKSGMKSLSSKEKALLDRASREL"
     gene            433..915
                     /locus_tag="PKK76_12700"
     CDS             433..915
                     /locus_tag="PKK76_12700"
                     /inference="COORDINATES: protein motif:HMM:NF028004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HNL69383.1"
                     /translation="MRIVWIGSWIVSVALLATVDCKPRTLTEVPMPDYKSFRHVKSGL
                     LLKGDADDLLTGIHHVYANDAAVEALRNRSDFPEDSVLVFDLFEPLDTPEGYKEGAHK
                     FTAVMHKHSSFKSTGGWGYEAFLPDGKRVVSDANKMCFECHKSVESQGYVFTNAGMSE
                     "
     gene            complement(993..1346)
                     /locus_tag="PKK76_12705"
     CDS             complement(993..1346)
                     /locus_tag="PKK76_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69384.1"
                     /translation="MNKFIVVLIALFVSGALLSQERNTKCKDELARICPGYGGDGNKL
                     GACLKDPEKSKQLSPECKPRRGGFPAIKASCSTEIQSYCSTATEDGKLYMCIRQQMNK
                     NPSSVGQNCKDQMNK"
     gene            1546..2058
                     /locus_tag="PKK76_12710"
     CDS             1546..2058
                     /locus_tag="PKK76_12710"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNL69385.1"
                     /translation="MKRKIPLFRILVASAVLFSAAGVFAEPPGPPPEEGGMFGPGNPR
                     EFGKLMDELKLTDAQRAKVKAQHEATKTDAKKYHEQLKPMHEELRALLEANTVDLKAV
                     RAKLQSISNVQLELRMIHIKGRLDFESVLTPDQKAQLKKITKERMDKMREHHGENRPH
                     RGPGAPDDER"
     gene            2055..2591
                     /locus_tag="PKK76_12715"
     CDS             2055..2591
                     /locus_tag="PKK76_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNL69386.1"
                     /translation="MTEAELTGAIEKSKVTALKSIRRHLPHDLDGLLDDIVQETYLRY
                     YMTFRDSRPLSDDYLHRWIYVAARNECKRAARKSRREGFAYAKLKPDAEAAANEENTS
                     VNEDMVPQDPENVRLQIEAMPDPFRETFLLRLGGLKMVTIAERLNVTVGTVKSRLARG
                     KRFLSRKGIASEDAGSQS"
     gene            2588..2908
                     /locus_tag="PKK76_12720"
     CDS             2588..2908
                     /locus_tag="PKK76_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69387.1"
                     /translation="MSQIERSPGYRRTEKKILSDSFSAELAARIVRDANARKRAPTQI
                     WTMGLAAAAVVVLAVGLFFYTQRTPDYPGLTSYSAQSVAAADQTNADWNETDSIISSA
                     LENR"
     gene            2994..3905
                     /locus_tag="PKK76_12725"
     CDS             2994..3905
                     /locus_tag="PKK76_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020773820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69388.1"
                     /translation="MTFALLGAFASVEAQSPRCSVFPADHIWNTRVDSLPVHKLSASY
                     VRSIGEKKALKADFGSGLWEGGPIGIPFTTQTGSPVQVQFEYADESDRGPYPLPSKPP
                     IEGGDASNGDRHVIVVDESKCKLYELYNARKTSRGWSAGSGAIFDLRTYVLRPAGWTS
                     ADAAGLPIFPGLVRYDEVASGEIKHAIRFTTNITQKAYVWPARHFASSRKDANLPPMG
                     LRLRLKSSYNMNGFSRETRVILQALKTYGMILADNGSPWFLSGAPDERWNNKTLRELG
                     RVTGENFEAVDATGLMMSADSGQIKKK"
     gene            complement(3944..>4358)
                     /locus_tag="PKK76_12730"
     CDS             complement(3944..>4358)
                     /locus_tag="PKK76_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69389.1"
                     /translation="FKDEFQGRIWAEKIEPGPELSVLARFTKGLYSGSPAVILQKAGR
                     GKWIHLCTVPERSREFFSWLAIHTLSRIECPPGVQAFPMNTNLGPAIGWINHGTDAEV
                     PWSGKLIQARFPDIMASPAMGRILAAGAIGVLLPD"
BASE COUNT         1119 a         1014 c         1161 g         1064 t
ORIGIN      
        1 gaatatctgc tgtattgctt gcctacgccg ttctatggcc ggatcgcaag gcacttttct
       61 ttttcgtcat ccccatgcga atgagatggg tcattctagt tttcgccgta ctgattgcag
      121 tttcttctat ggtggctctg ctgcaggttc tgggtggagc tctgggcggc gcgctcttca
      181 tctactatta cttgcgaaag gggaatcgta cttacggcca atctgtagaa gatagaacca
      241 tgactctctc agaaagatgg gaagagttta agaaaaaacg acgtctgaac aaaaagaaag
      301 cagagattga acgcagaatc gaaatgaaag aagaagtgga tcgtcttctt gaaaaaattt
      361 cgaagagcgg aatgaagtca ctttccagta aagagaaagc tctgctggat cgcgcatcca
      421 gggagttatg aaatgagaat cgtatggatt ggttcatgga tcgtgtccgt cgcactactg
      481 gcaacagttg attgtaaacc caggactttg acagaagtcc ctatgccgga ttacaagtcg
      541 ttcaggcatg tgaagtcagg cttgcttctc aaaggagatg cagacgattt acttacaggg
      601 attcaccacg tttatgcaaa cgatgcagcc gtagaagcat tgcgaaatcg atctgatttt
      661 ccagaagatt cggttctcgt gtttgatttg ttcgaacctc tggacactcc agaaggatac
      721 aaagagggcg cgcataaatt cacggccgtg atgcacaaac attcttcttt taagtccacg
      781 ggcggctggg gatacgaagc gttccttccg gatggaaaga gagtagtttc agacgcaaat
      841 aagatgtgtt tcgaatgcca taaatccgta gaatcgcagg gttacgtatt cacgaatgca
      901 ggcatgagcg aataacgttc gccaaatccc tgacgaaata ttgcaaaggc gtgtttcagt
      961 gccgaccgca gatcagcact gaaacgggtt tcctatttat tcatctggtc tttgcagttc
     1021 tgtcccaccg aagaaggatt cttattcatt tgttggcgaa tgcacatgta tagttttccg
     1081 tcttccgttg cggtcgagca gtaagattga atctctgtgc tgcagctggc tttgattgcg
     1141 gggaagcccc ctctgcgggg cttgcattcc ggggaaagct gctttgattt ttcggggtct
     1201 tttagacagg cacccagctt gttcccgtca ccgccgtagc ccgggcaaat tcttgccagt
     1261 tcatccttgc attttgtatt tctttcctgt gatagaagcg cgcctgaaac aaacagcgcg
     1321 atcaacacaa cgatgaattt attcatccaa ctgctccttt tttgatatgg gcgatttttt
     1381 tcagccccgt ggaatacaag caggtcatcc ggattttttg cgttcttttt gagaaattat
     1441 tcgcctggat tgcgtacgca tggatagtct ggatcagact gacgtgcgaa atggtgtctc
     1501 tagaaaatct ggggaacctt ttttcgcctg cgggtttata gagacatgaa acgaaaaatc
     1561 ccccttttca gaattctagt cgcaagtgcc gtgctgtttt cagcagcagg cgtctttgct
     1621 gaacctccgg gccccccgcc agaagaaggc ggaatgtttg gaccaggcaa tccacgtgaa
     1681 ttcggtaaac tcatggatga gctgaagctt acggacgcgc agcgtgcgaa agtaaaagcg
     1741 cagcatgaag ctacaaaaac cgatgcgaag aagtatcacg aacagctcaa gcctatgcat
     1801 gaggaactcc gtgcgcttct cgaagcaaac acggtagatt tgaaggcggt tcgagctaaa
     1861 ttgcagtcca tcagcaatgt tcagctggaa cttagaatga ttcacatcaa aggacgtttg
     1921 gatttcgaat ctgttctgac tccggatcaa aaagctcagt tgaagaagat cacgaaggaa
     1981 agaatggaca agatgcgcga acaccacggg gagaatcgtc cccatcgcgg tccgggcgca
     2041 ccggacgatg aaagatgacg gaagcggaac ttacgggcgc tatcgaaaag agcaaggtaa
     2101 ctgctcttaa gtcgattcgg cgccatctgc ctcatgatct tgatggtctc ctggacgaca
     2161 ttgtccagga gacttacctg cgctattata tgacgtttcg cgactcgcgt ccgttaagcg
     2221 acgactatct gcatcgctgg atctatgtcg ctgcgcgaaa cgaatgcaaa cgagcagcgc
     2281 gaaaatccag acgagaagga tttgcgtatg cgaagctgaa gccggatgca gaggcggccg
     2341 cgaacgaaga aaacacgagt gtgaatgaag atatggtgcc gcaggatccg gagaatgttc
     2401 gcttgcagat agaagcaatg ccggatccgt ttcgggaaac atttctgctt cgcctgggtg
     2461 gattaaagat ggttacgatc gcggagagac tgaatgttac tgttgggact gtgaagtcca
     2521 ggcttgcgcg tggaaagcgc tttctttcca gaaagggaat cgcgtctgaa gacgcaggga
     2581 gtcagtcatg agccagatag aaagaagccc gggatacagg cggacggaga agaaaattct
     2641 ttccgattct ttttcagcag aactcgccgc gcgcattgtg cgagatgcaa atgcaaggaa
     2701 gcgagcgccc actcaaatct ggaccatggg actcgctgcc gcagcagtgg ttgtccttgc
     2761 tgttggtttg tttttttata ctcagagaac tccggactat ccaggcctca cttcttattc
     2821 ggcgcaatct gtagctgctg cagatcaaac caacgcagac tggaatgaaa cggattcgat
     2881 cattagttct gctcttgaga ataggtgata caacatgagt gaaaacgtat gagtgaagca
     2941 attccagatt tccggatgag tgtatgaaga tgaatcttag atcatcgtgt tccataacct
     3001 ttgccctgtt aggtgctttt gcgagtgtgg aagcgcagag tccgcgctgc tcggtatttc
     3061 cggcagatca catctggaat acgcgagtag attcgctccc cgtccacaaa ctgtctgcct
     3121 cctacgttag aagcattggt gaaaagaaag cactgaaggc cgattttgga agtggtctat
     3181 gggaaggcgg gcccatcggg attccgttta ctacccagac cggttcacct gttcaggtgc
     3241 agtttgaata cgcggacgaa agtgaccgcg gtccatatcc gcttccatct aaaccgccga
     3301 tagagggcgg agatgcatct aacggagaca gacatgtgat cgttgtagac gaatcaaaat
     3361 gcaagctata cgaactgtac aatgcgcgaa agacgtcgcg cggatggagt gccggatccg
     3421 gggccatatt cgatttaaga acctacgtac ttcggccggc cggatggacg tctgcagatg
     3481 ctgcgggcct tcccattttt ccaggactgg tccgttacga cgaagtggcg tctggcgaaa
     3541 tcaaacacgc aatccggttt accaccaaca ttacgcagaa ggcatacgtc tggccggcgc
     3601 gacattttgc ttcgagcagg aaagacgcga atcttcctcc catgggactc cggctgcggc
     3661 tcaaatcttc ctacaatatg aatggatttt ctcgcgagac gcgcgtgatt ttgcaggcat
     3721 tgaaaacata cggaatgatc ctcgctgaca atggttcccc ctggtttctt tccggagctc
     3781 ccgatgaaag gtggaacaat aaaactctgc gcgagctcgg tcgcgtaacc ggggaaaact
     3841 ttgaagcggt ggatgcaact ggacttatga tgagtgctga ttccggacaa attaagaaga
     3901 agtaaccgcc acaggttcgg atgcgccgag atgtcgcata acgtcagtct ggaagcaata
     3961 ctccaatcgc acccgcggcg aggatccgcc ccattgcagg cgatgccatg atatcgggga
     4021 agcgagcctg aatgagtttt cctgaccatg gcacctctgc atctgttccg tgattgatcc
     4081 atccaatggc cgggcccaga ttcgtattca ttggaaaagc ctgcacgcct ggcgggcatt
     4141 cgatgcgcga gagtgtatga atggccaacc aggaaaagaa ttctctggat cgctcgggca
     4201 cagtgcacaa atgaatccac tttccgcggc cggccttctg cagaatgact gcgggcgaac
     4261 ctgagtagag cccttttgta aatcgagcga gaacggaaag ttcgggacct ggttcgattt
     4321 tttccgccca gattcgtccc tgaaattcgt ctttgaat
//
LOCUS       DAORFF010000325         4349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11851_length_4349_cov_3.500932, whole genome shotgun sequence.
ACCESSION   DAORFF010000325 DAORFF010000000
VERSION     DAORFF010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11851_length_4349_cov_3.500932"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..649
                     /locus_tag="PKK76_12735"
     CDS             137..649
                     /locus_tag="PKK76_12735"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNL69390.1"
                     /translation="MDYSGGIFNVEEIELVFPKFNNEAKMTESEICMKCHGCCTYVAV
                     PLEYPRSKDTLDTYVWYLLHRNVEIEIDNYNKWFLIFKTPCDKLLPNGACSIYETRPE
                     ICRDYSADNCSRTAKDHAHQFKQPSELLQYLEQKKKAKSGTVKPASASRRIAGKKSVR
                     MTGKKSALRK"
     gene            646..1311
                     /locus_tag="PKK76_12740"
     CDS             646..1311
                     /locus_tag="PKK76_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNL69391.1"
                     /translation="MKPFWISIGVKLLIEFWFLFIRAKIIVPPETEKLLAEKRGFILA
                     GFHNQIMSLTYHCSKYLGKKRKIKLTPLVSQSKDGEMIYQTFLRFQMYSERGSTSKGG
                     AAAFRALLKRIKDGWVPIFTPDGPRGPLYKVQPGVISLAAATGLPIVHFFSVFDRAYQ
                     TRSWDKMRLPKFGAKQWIEYSQPFYVPRKLSEEELEETIGRLEREMMQQVDRLETLVT
                     SKT"
     gene            complement(1295..1882)
                     /locus_tag="PKK76_12745"
     CDS             complement(1295..1882)
                     /locus_tag="PKK76_12745"
                     /inference="COORDINATES: protein motif:HMM:NF025595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HNL69392.1"
                     /translation="MEESQKSPEEKARDAKDQLNNWVAVAVALIATFTGITKIKDDNI
                     VQAMLQMKSDAVDRWNESQASKIKLHIAENGLRQTQIASLTAGGAQRQALNTAAKEYE
                     AAIEKYRKRAEEASAEAKKNEEAYDSLGYRDDQFDMSDMFLSIAMSLLAVSALTGKRW
                     LFYFSMVLSTFGVIMGVAAFVQLHIHPNFLVKFLT"
     gene            complement(1915..2319)
                     /locus_tag="PKK76_12750"
     CDS             complement(1915..2319)
                     /locus_tag="PKK76_12750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNL69393.1"
                     /translation="MNLDDLSRLESLCFPDPWTREMLEGSVARPDSIVIFRKINEQIS
                     AYLLATSSENGIELLRIGVAPSGRRLGLAKSMIMELVAMSQRILLEVSDSNRAAISLY
                     LGCGFKEIARRKRYYADGSDCIVMQLEKRSGF"
     gene            2376..3278
                     /locus_tag="PKK76_12755"
     CDS             2376..3278
                     /locus_tag="PKK76_12755"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNL69394.1"
                     /translation="MERFILASCLPYRSAIYRMGGFQAFPNDFLKFYTKEEIETARID
                     ASRALEILEKDKDLRFISILDERYPASLRNIYDPPPGLYICGADLDGEFDAIVGTRAP
                     SPVVRCAVRILVRSLSSMIVSGFARGVDAEAHLECARGGRRTIAVLGSGHRFAGPGST
                     LRLLRPYNDFTTFVSEFPPHWPARSHSFPRRNRIIAGLSARVFLMQAPAKSGALITAR
                     YALEEGRDLICFDHPVLETWMNEGARRLIQEGATLLEIEALDSLFVRPPGIVWPPGNE
                     QLEFFEKRQRGLLRVSDTLYIEEV"
     gene            3327..>4349
                     /locus_tag="PKK76_12760"
     CDS             3327..>4349
                     /locus_tag="PKK76_12760"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNL69395.1"
                     /translation="MAVKTKEETAPKKKEAKKSIKASSKGKTLVIVESPAKAKTINKY
                     LGNSYIIEASMGHLIDLPRSRMAVNVEQNFEPEYITVRGRAKILNKLKKIAQHSDRVL
                     LAADPDREGEAISFHLARALGPANTDIKRIEFNEITKSAVQEAVRHPRDINMDLVNAQ
                     QARRILDRLVGYNISPLLWKKIKRGLSAGRVQSVALKLICDRESEIDKFVPEEYWTLD
                     VKFNVGKKGFAASLSSVDGKKADLKSKEEVDSILARMEGLSYNVASVTTKERRRQPPA
                     PYTTSRLQQDGTNRLGFTSQKTMMVAQQLYEGLELGGTTTGLITYMRTDSTRVSPGAV
                     EQLRGYI"
BASE COUNT         1111 a         1062 c         1082 g         1094 t
ORIGIN      
        1 gaacagcggc cgatctactt tcgcttgcag atgaaatcaa aggagaagtg ctatcacgct
       61 ttggaattca actcgagatg gaacctgaat acatcgtttg attgcctccc tgagcgttta
      121 gaatttcctg gcccggatgg attattccgg tggcattttt aatgtagaag agatcgagct
      181 cgtctttccg aagttcaaca acgaagcaaa aatgacggag agcgaaatat gcatgaagtg
      241 ccacggttgt tgcacgtacg ttgctgttcc tctcgaatat ccaagatcaa aagatactct
      301 ggatacatac gtctggtact tgctacacag aaacgtagaa atagaaatcg acaactacaa
      361 taaatggttt ctgatcttta agactccttg cgataagctc ctaccgaacg gcgcatgcag
      421 catttacgaa acgcgcccgg agatatgccg ggattattca gcagacaatt gctctcgcac
      481 cgcgaaagat catgcacatc aattcaagca accatcggaa cttctacagt atctggagca
      541 aaagaagaaa gcaaagtcgg ggacggtaaa gcctgcatcc gcttcgcgca gaattgccgg
      601 taaaaagagt gttcggatga ctggaaagaa atcagcattg cgcaaatgaa gcctttctgg
      661 attagcattg gcgtaaaact gctgatagag ttctggttct tgttcattcg tgcgaagatc
      721 atagtcccac ccgaaacaga aaagctcctc gcggagaaac gcggattcat tctggccgga
      781 tttcacaatc aaatcatgtc tttgacgtat cattgttcca aatatcttgg aaagaagcgc
      841 aagatcaaac ttactcctct ggtttcgcaa tcaaaggacg gagaaatgat ttatcagacg
      901 tttctgcgct ttcaaatgta ttcagagcga ggctctacga gcaaaggcgg agcggcagcg
      961 tttcgcgctc tgctcaaacg aatcaaggat ggctgggtgc cgattttcac tccggatggc
     1021 cccagaggcc cgctctacaa agtacaacct ggagtcattt ctctggcggc ggctaccggt
     1081 ctccccatcg ttcatttctt ttccgtattt gatcgcgcgt atcaaacaag aagctgggat
     1141 aagatgaggc tgccgaagtt cggcgcgaag cagtggattg aatattcaca gcccttctat
     1201 gtgccgcgaa agctgagcga agaagagctt gaggaaacga tcggccgtct ggaacgcgaa
     1261 atgatgcagc aggtcgatcg cctcgaaact ctcgttacgt caaaaacttg accaggaaat
     1321 tcgggtgaat gtgcagttgc acaaaagccg ccactcccat gatcactcca aacgtactta
     1381 gaaccatgga aaaataaaag agccagcgtt ttccagtcag tgcggagacg gccagtagag
     1441 acatggcaat cgaaagaaac atatctgaca tgtcgaattg atcatctctg tatccgagcg
     1501 aatcgtatgc ttcttcgttt ttctttgctt ctgcgcttgc ttcctctgcg cgttttctgt
     1561 atttctctat ggcggcttcg tattcttttg cagctgtatt cagagcctgt ctttgtgcgc
     1621 ctccggctgt cagagatgcg atttgagtct gcctgaggcc gttttccgca atgtgaagtt
     1681 tgattttaga tgcctgcgat tcattccatc tgtccacagc gtctgatttc atttgcagca
     1741 ttgcctgcac tatgttgtcg tccttgatct tggtaattcc ggtaaacgtg gcaatgagag
     1801 ccacggcgac ggccacccag ttgttgagct gatctttcgc gtcccgtgct ttctcctctg
     1861 gcgacttttg tgactcttcc atagaaactc cttgcgggag ttacgattga gcattcaaaa
     1921 accggacctt ttttctagct gcatgacaat gcaatcggac ccgtctgcgt agtaccgttt
     1981 tcttcgcgca atttccttaa atccacaacc aaggtagagg gagatggcag cgcggtttga
     2041 atcagacact tcgagtagaa tcctctgcga catggccact agctccatga tcattgattt
     2101 tgcaaggccc agccttcttc ctgaaggggc aacgccgatt cgcagtaact cgattccgtt
     2161 ctccgaggat gtggccagca aatacgcact gatttgctca tttattttcc gaaatattac
     2221 gatgctgtct ggcctggcca cgcttccttc gagcatctct ctggtccagg gatccgggaa
     2281 gcaaagcgat tcgagccggc tgagatcatc caggttcacg tgtcctcgta cctgtttttt
     2341 tcgaaacgca ccattctttt tttaccatag aaaagatgga acgattcata ctggccagct
     2401 gccttcctta tcgatctgcc atttatcgca tgggcggatt tcaggcgttt ccgaatgatt
     2461 ttctgaagtt ttatacaaag gaagaaatcg aaacagcaag gatagatgcg tcgcgtgccc
     2521 tggagattct agaaaaagac aaagatcttc gctttatttc gattctggat gaacgttatc
     2581 cggcatctct acgcaacatc tatgatccgc ctcctggatt gtatatttgt ggagcagatc
     2641 ttgacggcga gtttgatgcg atagtcggca cgagagctcc gtccccggtc gtgcgatgcg
     2701 ccgtgcgaat tttagttaga tctctctctt ccatgattgt ttctggtttt gccagaggcg
     2761 tggatgcaga ggcgcatctg gaatgcgcgc gcggaggcag acgaaccatc gcagttctcg
     2821 ggtccggtca cagattcgcc ggacctggct ccacccttcg tctccttcgt ccgtacaatg
     2881 atttcactac atttgtttct gaatttccgc cgcactggcc cgcaagatcg cacagttttc
     2941 ccaggagaaa tcgaatcatc gccggacttt ctgccagagt atttctgatg caggcgcctg
     3001 cgaagagtgg cgctctgatc accgcacgtt atgctttaga agagggtcgg gacctgattt
     3061 gctttgatca tcctgtgctt gagacctgga tgaatgaggg agccaggcga ctcatccagg
     3121 aaggggccac attgctcgag attgaggctc tggattctct atttgtgcgt ccaccgggga
     3181 tcgtctggcc gcccgggaat gagcagctgg agtttttcga aaaaaggcag agggggttgc
     3241 ttcgtgtctc cgataccctt tatatagaag aagtttaaaa aaagagtggc gcgattgcgc
     3301 ggccgggtcc ccggagacat aaagtcatgg cagtaaaaac aaaagaagag acagctccga
     3361 aaaaaaaaga agccaaaaag agcatcaaag catcctccaa aggcaaaact ctcgtcatcg
     3421 tagaatcgcc tgcgaaggca aagacaatca acaaatacct ggggaatagc tacatcattg
     3481 aagcctccat gggccacctg attgacctgc cgcgttcgcg tatggccgta aatgtggagc
     3541 agaatttcga accggaatac atcaccgttc gcggccgcgc caagatcctg aacaagctga
     3601 agaaaatcgc acagcattcc gatcgcgttc tacttgcggc ggacccggat cgcgaaggag
     3661 aagccatttc atttcacctg gcgcgtgccc tcggcccggc caacacggac atcaaacgaa
     3721 ttgaattcaa tgaaattact aagagcgccg ttcaagaagc tgtgcgtcat cctcgcgaca
     3781 tcaatatgga tctggtcaac gcgcagcagg cgcgtcgtat cctggacaga cttgttgggt
     3841 ataatatttc tccgttgctc tggaagaaga tcaagcgcgg cctctctgcc ggtcgggttc
     3901 agtctgttgc gttgaagctg atttgtgatc gagaaagtga aattgataag tttgttccgg
     3961 aagaatactg gaccctcgac gtaaaattca atgtaggtaa gaaaggattc gcggccagcc
     4021 tttcctcagt ggacggaaaa aaggcggatc tcaagtcaaa agaggaagtg gattccattc
     4081 tggccagaat ggaaggattg tcgtataacg ttgccagcgt gactacaaaa gagcgcagac
     4141 gtcaaccacc tgcgccttac acaacatcgc gtcttcagca agacggaacg aacaggctgg
     4201 gctttacttc tcaaaaaaca atgatggtgg cacagcagct ctacgaaggc cttgaactgg
     4261 gtggaactac caccggtctg attacttaca tgcgtactga ctccacacgc gtttctcctg
     4321 gagctgtaga acagttgcgt ggctatatc
//
LOCUS       DAORFF010000326         4340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11892_length_4340_cov_3.708285, whole genome shotgun sequence.
ACCESSION   DAORFF010000326 DAORFF010000000
VERSION     DAORFF010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11892_length_4340_cov_3.708285"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..829
                     /locus_tag="PKK76_12765"
     CDS             62..829
                     /locus_tag="PKK76_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNL69396.1"
                     /translation="MLIQESITDFIAEIKLQINETNSFTPDGFAALEGALTKMAADNS
                     VRVLILSSARPDFFSNGLDPHSVHGATPEQIQKLISSFFSVLKKIFLFPVPVIAAING
                     HAVGYGAMLGLMSDFRLLVDKGARVSFPELNIGVSLPIFVTTALEDLVGLRATRDLLF
                     TGLAPKPPEALALGLVDELVEKEKLADRARALGKKIASLPKNATRAQKGIIRHRFAQN
                     LDRILEEDIKQTGGLLNSPEAREGFAAMVEKRRPKFD"
     gene            840..3176
                     /locus_tag="PKK76_12770"
     CDS             840..3176
                     /locus_tag="PKK76_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNL69397.1"
                     /translation="MTAPSILITQCLQNDFVRLIDRYEALPNLLHVGYDESMRLLGER
                     MEEGPVRSMLHWAYERSPEELAIIHIRDWHNPSDPAQKDHLKQFGPHCVQDTEGARFV
                     FEESLDPARHTIVNASGLNDFVDTTLEEVLRPYRGKPVRVGIAGVWTEAKVTFLAYDL
                     RTRYPEFEIAVASPLTASSSRQMHFIALDQLKSILGVRVFSSLGGFTEFLTGSIPDIG
                     SRIPFREGLLHLDGSYSVSETDKSILTYLFRDCKKVELKCLDGGFSGNVVLKARSVDV
                     LGHPQVPTVIKIGARDPIAQERTSFERIEEVLGNNAPQIVDFAEIGERGAIKYRYAAM
                     LDGPVHVFQDLYESIEEEIASGKKHLSEETLKPVFDVLDTVFLAQLGRLYQAGEPEKV
                     DLLQYYDFSTRYAPGVRRRITALIGGNPEDIGEVFPGRNIENVCPFYEKDLEHLHSTR
                     TSARMAFVHGDLNGRNIVLDPSHNVWMIDFFHAHRGHILRDLLKLENDIFYIFTKIAS
                     ISELEEAVRVTDFFLSQPDLGVPPLPGALSISNPGLKMAALTVAHMRSYYPALVDKDR
                     EPVQMQIGLLRYAMHTISFDECNEFQKKWALASGSALITKIKESFARSSRLRIDFMET
                     GEPGKIGMTILPGRKDRERVLSEDIAAIKESKIASVLCLITENEFSEYGVPDLKQEYE
                     KAGLATRYLPILDQAIPTREELSSALSWVHDELKQSKNVLVHCVGGLGRSGLAAAAYL
                     ILEKNMSPDRAIETVREARGRRAIETRKQEEFLESLTR"
     gene            complement(3210..3458)
                     /locus_tag="PKK76_12775"
     CDS             complement(3210..3458)
                     /locus_tag="PKK76_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69398.1"
                     /translation="MAKLLLSLPLILGLLSYCSAQSCDAKRQQSENCKVASLPLLATC
                     PSASTTSNQNTCYAAWAAAIVACDAIVPPYCAKPSSDD"
     gene            complement(3502..>4340)
                     /locus_tag="PKK76_12780"
     CDS             complement(3502..>4340)
                     /locus_tag="PKK76_12780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNL69399.1"
                     /translation="AAQRAAFFLPMFHNQTAVITGGSSGIGLALAKELRLRGARVVLI
                     SRSGVKLKKAQTVLAEIPGDPVNVFRCDVRNYRQLRRVFQEIEKSGPVSILINSAGMV
                     HPGKFETIPVSLFRRTMAANVLGTVHACRAAAPAMMSRHAGTIVNIGSLAGMIGVFGY
                     TAYSASKFAVHGFTQALRAEMKPHGIHVMLAAPPDTDTPMLHEERKLLPRESAAINAS
                     GGLLSPKAVALAIIRGIEKRRSIVVPGFEGKFAYYASRFIPGIVDAVMDHKIRKAQSG
                     KP"
BASE COUNT         1119 a         1141 c         1088 g          992 t
ORIGIN      
        1 cacggccggc gtacccgtgc gaaacgaata gacttctttt gcagcgaagc gaggctcggc
       61 catgctgatt caagaaagta tcacagactt catcgcagaa atcaaactcc agatcaacga
      121 gacgaactct tttactcccg acggatttgc cgcacttgag ggtgcgctca caaagatggc
      181 ggcggacaat tcggttcgcg tcctcattct atcaagcgcg cgcccggatt tcttttccaa
      241 tggactggac ccgcactctg tgcatggtgc aacgcccgaa caaattcaaa aactcatatc
      301 gagtttcttt tctgtgctta agaagatctt tctatttcca gtgcccgtga tcgccgccat
      361 aaacggacat gcggtgggct acggcgccat gctaggactc atgagcgact tcaggctgtt
      421 agtcgacaaa ggagctcgcg tttcattccc ggaattgaat atcggagtga gccttcccat
      481 ctttgtgacc acggctcttg aagatctggt cggcctgaga gccactcgcg acctattgtt
      541 caccgggctt gcgcccaagc ctccggaagc tctggctctg ggacttgtag atgaactcgt
      601 tgaaaaagaa aaactcgcgg accgagcaag agctctcgga aagaaaatcg catctctccc
      661 caagaatgct acccgagctc aaaaaggaat cattcgccat cgcttcgcgc aaaacctgga
      721 tcgcatcctc gaagaagaca tcaaacaaac aggtggcctg ctcaattctc ccgaggcacg
      781 cgagggattc gctgccatgg tagaaaaaag acgtcccaaa ttcgactaac gttagcatca
      841 tgactgctcc atcgatcctc attacgcaat gcttgcaaaa tgattttgtt cgcctcatcg
      901 atcgctatga ggctttaccc aatctactgc acgtaggcta tgacgaatcc atgcgccttc
      961 ttggtgaacg gatggaagaa ggaccggttc gctccatgct acactgggct tacgaacgtt
     1021 cgccggaaga gctggccatc attcacatcc gggactggca caatccatcc gatcccgccc
     1081 aaaaagacca tctcaaacaa ttcggaccac actgtgtcca ggatacagaa ggcgcacgct
     1141 ttgttttcga ggaaagtcta gacccggctc gtcatacgat tgtaaatgca tccggactga
     1201 acgattttgt agacacaaca ctcgaagaag tattgcgacc ttatcgcggg aaaccagttc
     1261 gtgttggtat cgcaggagta tggacagaag caaaagtaac atttcttgcg tatgatctca
     1321 ggacccgtta tccggaattc gagatcgctg tcgccagtcc actcactgct tcttcgtctc
     1381 gccagatgca cttcattgcg ctcgatcagc tcaaaagcat cctgggcgtt cgagtgttct
     1441 catcactcgg gggattcaca gaatttctca caggttcgat tccggacatt ggaagtagaa
     1501 tcccattccg cgaaggattg ctccacctgg acggaagcta cagcgtcagt gaaacagaca
     1561 aaagcatcct tacgtatctg tttagagact gtaaaaaagt agaactcaaa tgtctcgatg
     1621 gcggtttcag cggcaatgta gtgctcaagg caagaagcgt ggacgttctg ggtcatcccc
     1681 aggttcccac tgtgatcaag atcggagcac gcgatccgat cgcgcaggaa agaacgtcgt
     1741 ttgaacgaat cgaagaagtc cttggcaaca acgcacctca gattgtcgat ttcgccgaaa
     1801 tcggagaacg aggtgctatc aaataccgtt atgcggccat gctcgatggg cccgttcatg
     1861 tatttcagga tctctacgaa tccattgaag aggaaattgc ttcaggaaag aaacacctga
     1921 gcgaagaaac tctgaagcct gtatttgatg ttctggacac agtgtttctg gcccagctgg
     1981 gacgactgta tcaggcggga gaacccgaga aagtggacct gctccagtac tatgattttt
     2041 caacgcgcta cgctccaggc gtgcgccggc ggatcacggc actcattggc ggaaaccccg
     2101 aagacattgg cgaagtcttc ccgggaagaa atatcgaaaa cgtgtgcccg ttttatgaaa
     2161 aagatctcga acaccttcat tctacccgca catctgcgcg gatggctttc gttcacgggg
     2221 acttaaacgg gcgcaacatt gttctcgatc cttcgcacaa tgtatggatg atcgatttct
     2281 ttcacgctca tagaggccat atccttcgcg atctcttgaa gctggaaaac gacatcttct
     2341 atatcttcac caaaattgcg tccatttccg aacttgaaga agcagtgcga gtcacggact
     2401 tcttcctatc acaaccagat ctgggagtcc ctccgttacc aggcgcactt tcgatttcga
     2461 atcctggact caagatggcg gcactcaccg tcgcccatat gcgatcctac tatccggctc
     2521 tcgtggacaa agaccgggaa ccggtgcaga tgcagattgg ccttcttcgc tacgcaatgc
     2581 acaccatctc atttgatgaa tgcaacgaat ttcaaaagaa atgggcgctg gcgtcggggt
     2641 ctgcgctgat cacaaaaatc aaagagagct tcgctcgcag cagtcgtctt cgcatcgatt
     2701 tcatggaaac gggagaacca ggaaagattg ggatgaccat attgcctgga cgtaaagatc
     2761 gcgagagagt tctttctgag gacattgcgg cgatcaagga aagtaagatc gcgtctgttc
     2821 tttgcctcat cactgaaaac gaattttcgg agtatggtgt tccagatctt aaacaggaat
     2881 acgaaaaggc cggattagca acacgttatc tgcccattct agaccaggca attccaacca
     2941 gagaggaact gtcgagcgcg ctctcctggg tacatgatga actgaagcaa tcaaagaatg
     3001 tgcttgtgca ttgcgtcgga ggccttggaa gatctggtct tgctgccgcc gcctatctaa
     3061 tcctggaaaa aaacatgagt ccggatcgcg ccatagaaac ggtccgggaa gcaagaggaa
     3121 gacgcgctat tgaaacgcgc aaacaggaag aattcttaga aagcctgacc cgttaggcga
     3181 tcaagctccc atcaacaatc aagatcccat caatcgtctg aggatggttt ggcacaatac
     3241 ggaggaacga ttgcatcgca tgcaacgatc gccgcagccc aggcagcata acatgtgttc
     3301 tgattcgaag tggtcgaagc agatggacag gtagccagaa gtggaaggct ggccactttg
     3361 cagttttcag actgctgtct ctttgcatca cagctctgcg cgctgcaata agacagcaga
     3421 cccagaatca atggaagtga cagcaggagt tttgccatag tcatctgtag ctcactgccg
     3481 ggagcctgca agccttttga ttcagggttt gcctgactga gctttgcgaa tcttgtgatc
     3541 catcacagca tccacgattc ctggaatgaa cctggacgca tagtaggcaa attttccctc
     3601 aaagcctggc acaacaatgg accggcgttt ttcaattcct cggatgatgg cgagggctac
     3661 cgccttcgga gatagaagtc caccggatgc attgatcgct gccgattctc tgggcaacag
     3721 cttgcgttct tcgtgtagca tgggggtgtc cgtgtccggc ggtgctgcga gcattacgtg
     3781 aatgccatgc ggcttcatct ccgcgcgaag ggcctgggtg aagccatgca ctgcaaactt
     3841 ggaggcgcta tatgcagtgt agccaaagac acctatcatc cctgccagag agccaatgtt
     3901 gacaatggtt cctgcatgtc tggacatcat ggcaggcgcc gccgcacgac atgcatgcac
     3961 cgttccaaga acgttagccg ccattgtgcg acgaaagagg gaaacaggaa tagtctcgaa
     4021 ctttccagga tgaaccatgc ccgcggaatt gatcagaatg ctgactggtc cggatttctc
     4081 gatctcctgg aatactcgac gcagctgccg atagttacga acatcacagc gaaagacatt
     4141 tactggatct cccgggattt ccgctagaac tgtttgcgcc ttcttcagct tcacaccgct
     4201 acgcgaaatc agaactacgc gcgcgccgcg caggcgaagc tcttttgcaa gtgccaggcc
     4261 aatcccgctc gatccgccag tgatgaccgc tgtctggttg tgaaacatag gcaaaaaaaa
     4321 agcggccctt tgagccgctt
//
LOCUS       DAORFF010000327         4327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11952_length_4327_cov_3.415730, whole genome shotgun sequence.
ACCESSION   DAORFF010000327 DAORFF010000000
VERSION     DAORFF010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11952_length_4327_cov_3.415730"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PKK76_12785"
     CDS             <1..937
                     /locus_tag="PKK76_12785"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HNL69400.1"
                     /translation="FQIGTGKFGVGEEAVLPDGTATRKLNKGLLQELVRNHPNIRMIQ
                     IKISQGAKPGIGGHLPADKVTPEIADVRRVTPYKTVISPPQHAELRASSPKEAVSKLI
                     EFIEEIRKLTELPVGIKLCVGRLDELDLLVLAMKKTGKGPDAIQVDGADGGTGAAPNL
                     FVNYVGYGSSIETLQYLDKLLKKHRIRDRVRLSSSGRIFTPAHAAYAFASGADTCETA
                     RAAMLSIGCIQAQQCHTNKCPTGITTNSAWRMRGIHIPEKSTRVHHFLKGFHEDMLEL
                     TRVMGHSDPRDIKLDDLRVMSRGGAFSAFFSDSHV"
     gene            930..1946
                     /locus_tag="PKK76_12790"
     CDS             930..1946
                     /locus_tag="PKK76_12790"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNL69401.1"
                     /translation="MFNRVIRIAGWLGINTVLGTMNGLLISFYSPEPFARVFLTAQIM
                     THVLCLLIEFAMFTAGIQLYRIGTHKKQPVRYFTAALCITAISAVCGVLAGGRIHQEL
                     LGFNPYENSRFYNVIFGSIIFAIGLASIDRFFRYLRETRLLAERSLQEARLAVLQERM
                     RPHFLFNTLNSIHAMLPDHADRADAAILMLADSYRFLLGPAEKPLVSFMEEWEFCKNY
                     VELMKVRFGERLSVWMEHRGDFSRVLIPPLSIQPVIENAFRHGVQKIPGNAIVQISAE
                     CAGSSVRIEVKDNGPGLPSDFESRSIDNIRSRLRFHFPGADLEVSNLGESGVSSRITL
                     TGAA"
     gene            1946..2644
                     /locus_tag="PKK76_12795"
     CDS             1946..2644
                     /locus_tag="PKK76_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNL69402.1"
                     /translation="MPLRCYVIEDEPPAREILFRFIEARKDLSLAGWAGDARSALNYL
                     KVNPADLVFLDVQLPGMSGFEMLEELRPSPAVIFTTAGQAHAVPAFDHGAVDFLLKPF
                     SMERFDIAVNRVVQKRTTNPSSGGLAVTEGENHYLIPFGEIIYLSSRGKATAVHTTDR
                     DFESQRILKDIESRLPESFLRIHKQYILNLDFLAHLKYRVGGGYTATLRDSDETSVPV
                     GRGFAGALRARWSG"
     gene            complement(2645..>4327)
                     /locus_tag="PKK76_12800"
     CDS             complement(2645..>4327)
                     /locus_tag="PKK76_12800"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNL69403.1"
                     /translation="QQRALDQERMTREAEEELLQHLRQMENLQKDFLALAIEEPGRTV
                     ESLAEKILETLAHLLRFDRGFITLLDRMDRYVSRGIGEVPAYVADFVPRAFHVIRRFK
                     PPEDFFREITAIADLQDQSGIPFKDPDGMIRTYSENANKIRAFLKRLTQDGFRTVIPL
                     TFKSELLGYMVGGASADSQMRYSDKNLLEAFRFSAALAVRNAMLYDELSLLKGKAEES
                     VQQLSEYIVDMHNAREHKMSEKTFVYLSKSMNDLVDRTRNLAGKNQPMLILGETGTGK
                     EMVANLIHDASMPESPFVAVNCAAIPGNLWESEVFGHVKGAFTDAKTDRPGKIEQAAG
                     GTIFFDEIGEMPLEMQPKMLRLIQERVFQRVGGTKEIKTDCRFIFATNRNLEEMQAKG
                     TFRSDLYYRINVFQIRIPPLRERRADIPALAQFFTEKYCAEMGYPARKLGDGAMDALC
                     RFDWPGNVRELENAVIQAIVNSTTDIIRREDLPVQLLDARKMRSESPAEIQKPSPGNQ
                     WDGRPLEEILNEHARRIIESALAKTKGNKVEAAKILGLKRATFYNRLKELGM"
BASE COUNT          999 a         1047 c         1123 g         1158 t
ORIGIN      
        1 gtttcagatt ggtaccggga aattcggcgt gggagaagag gctgttcttc cggatggaac
       61 agccactcgc aagctcaaca agggactcct tcaagaacta gtgcgaaatc atcccaatat
      121 ccggatgatt caaatcaaga tttcgcaggg cgcgaaacct ggaattggag gacatctccc
      181 tgcagacaaa gtaacacctg aaattgcgga cgtcaggcga gtgacgccgt ataagactgt
      241 gatcagcccg ccgcagcatg cggagcttcg agcatcgagt ccgaaggaag cagtctccaa
      301 actcattgaa ttcatcgaag agattcgcaa gctcacagag cttccagtcg gaatcaagct
      361 gtgcgtgggg agactcgacg agctggattt gcttgtactg gccatgaaga aaactgggaa
      421 agggcccgat gcaattcagg tagatggtgc agatggcgga acaggtgctg cacccaattt
      481 atttgtaaat tacgtaggat acggaagttc cattgaaaca ctccagtatc tggataagct
      541 tcttaaaaag cacagaatca gggatcgcgt tcgcctgagt tcgtccgggc gtatcttcac
      601 tccagctcac gcagcctatg cgtttgcaag cggtgcggat acatgcgaaa ctgcgcgcgc
      661 tgccatgctt tcgattggtt gcatccaggc gcagcagtgt cacacaaaca aatgtcctac
      721 aggcattaca accaacagtg cctggaggat gaggggaatt catattccag aaaaatcaac
      781 gcgcgtgcat catttcttga aaggctttca cgaagacatg ctcgagctga cacgcgtcat
      841 gggtcattca gatccgcgtg atattaaact ggatgacctt cgcgtgatgt cgcggggtgg
      901 agcgttctcg gcgttcttta gcgacagtca tgtttaatcg cgtcattcgc atcgcgggct
      961 ggctcgggat caataccgtt cttggaacca tgaacggtct tttgatttct ttttactcac
     1021 cggagccttt cgcgcgcgtt tttctcacgg ctcaaatcat gacgcatgtg ctctgcctgc
     1081 tcatagagtt tgcgatgttt acggccggga ttcagcttta tagaatcggg acgcataaga
     1141 aacaaccggt tcgctacttt actgcagcgc tctgtatcac tgcgatctcc gctgtgtgcg
     1201 gtgtgcttgc tggcgggaga attcaccagg agctacttgg gtttaatcct tatgagaaca
     1261 gtcgcttcta caatgtgatc tttggatcca tcatctttgc cattggcctg gcaagcattg
     1321 accgcttctt tcgctatctg agagagacca ggcttcttgc agagagatcg ttgcaggaag
     1381 cgcgtctggc agtactccag gaaagaatgc gcccgcattt tttgtttaat acactgaaca
     1441 gcattcatgc gatgcttcca gatcatgcgg accgagcgga tgcagccatc ctgatgctcg
     1501 cggacagcta tcgcttcttg ctcgggccgg cggaaaagcc gctcgtgagt tttatggaag
     1561 aatgggagtt ctgcaaaaac tacgtagaac ttatgaaggt aagatttgga gaaaggttga
     1621 gtgtttggat ggagcacagg ggcgattttt cgcgcgtctt gataccacct ttatccatcc
     1681 aaccagtcat cgaaaatgca tttcgccacg gagtgcagaa gatacctgga aacgcgatcg
     1741 tgcaaatttc tgctgaatgc gccgggtcat ccgtgcgaat tgaagtcaaa gacaatggac
     1801 cgggtcttcc ctcagatttt gaatccagga gcattgacaa tattcgctct cggttacgct
     1861 ttcatttccc gggcgcggat ctggaagttt caaatctggg tgagagtggt gtatcgagca
     1921 ggataacgct cacgggggct gcgtaatgcc tttgcgatgt tatgtgatcg aggacgaacc
     1981 acccgcacgt gaaattctgt ttcgctttat tgaggcgaga aaagatctct ctctggcagg
     2041 atgggcggga gatgctcgca gcgctctgaa ctatctgaaa gtgaaccctg cggatctagt
     2101 cttcctggat gtgcaattgc caggtatgtc cgggtttgaa atgcttgaag agcttcgccc
     2161 cagtcctgcc gtcatattta ctactgccgg ccaggcgcac gcagttcccg catttgatca
     2221 cggggccgtt gattttctat tgaagccttt ttctatggag cgatttgaca ttgcagtcaa
     2281 tcgggtcgtg cagaaacgga ccacgaatcc atcgagcggt ggactggctg tcacagaagg
     2341 agaaaatcac tatctgattc cttttggaga aatcatttat ttatcttcgc gtggaaaggc
     2401 aactgcggtt cacacaacgg atcgcgattt tgaatcacag agaatcttga aggacatcga
     2461 gtcgcgtctg cccgaaagct ttttacgaat tcataagcag tacatattga atctggattt
     2521 tctggcgcac ctgaaataca gagttggcgg tggatatacg gccactctgc gtgattcaga
     2581 cgaaacaagc gttccagttg gtcgcggatt cgctggcgcc ctgcgcgcca ggtggtccgg
     2641 ctagctacat tccaagttct ttcagacgat tgtagaaagt ggcgcgcttg agacccagga
     2701 ttttggccgc ttcaaccttg tttcctttcg ttttcgcaag tgcactttca atgattctcc
     2761 ttgcgtgttc attcagaatt tcttcgagag ggcggccatc ccactggttg cccggtgaag
     2821 gtttttggat ttcagccggc gattccgatc tcatttttcg ggcatcgagc aattgcacag
     2881 ggagatcttc tcttcgaatg atgtccgtgg tgctattcac aatcgcctgg atgactgcgt
     2941 tttcaagttc gcgcacgttt cctggccagt caaaccggca cagagcatcc atggctccat
     3001 cgcccagttt cctggcaggg tagcccatct cggcacagta cttctccgta aagaattgag
     3061 ccagagcagg aatgtctgct cttcgttcgc gcaagggcgg gatgcgaatc tgaaagacat
     3121 tgattctata gtagagatca gatcggaacg ttccctttgc ctgcatctct tccaggtttc
     3181 gattggttgc gaaaatgaat ctgcaatccg tcttaatttc ttttgttccg cccacccttt
     3241 gaaagacgcg ctcctgaatg agtcggagca ttttgggctg catttcgagc ggcatttcgc
     3301 cgatttcatc gaagaagatg gttcccccgg cagcctgctc aatctttcca ggtctgtctg
     3361 tttttgcgtc cgtgaacgca cccttcacgt gtccgaatac ttctgactcc cagaggttcc
     3421 cgggaatggc cgcgcagtta accgcgacga aaggagattc tggcatggac gcatcatgaa
     3481 tgagattggc caccatctct tttcctgttc cagtttctcc gagaatcagc atgggctgat
     3541 ttttccccgc gagatttcgc gtacgatcca ccagatcgtt catggacttg ctcaaataga
     3601 cgaatgtctt ttcagacatc ttgtgttcgc gcgcgttgtg catatccaca atgtattccg
     3661 aaagttgttg cacgctttcc tctgcttttc ctttcagcag agagagttca tcatatagca
     3721 ttgcgttgcg aactgcaaga gccgctgaaa aacgaaatgc ctcaagaaga ttcttgtccg
     3781 aatatcgcat ttgcgaatcg gcagacgcgc cccctaccat gtatcccaga agttctgatt
     3841 tgaaagtgag tggaatgact gtgcgaaagc catcctgagt cagtcgtttg agaaaggcac
     3901 ggatcttatt tgcgttttcg gagtaggtgc gtatcatgcc gtctggatct ttgaaaggaa
     3961 ttccgctctg atcctgaagg tcagcgatgg cggtaatttc tctgaagaaa tcttcaggtg
     4021 gtttgaatct gcgaatgacg tgaaacgctc tgggaacaaa atctgcgaca tacgcgggta
     4081 cctctccaat cccgcgtgaa acatatctgt ccattctatc caggagtgtg atgaatcctc
     4141 gatcgaaacg caacaggtgt gccagtgttt cgaggatttt ttctgcaagt gattctactg
     4201 tgcgtcctgg ttcttcgatg gcaagagcca gaaaatcttt ttgcaggttt tccatctggc
     4261 gaaggtgttg cagaagttct tcttctgctt cgcgcgtcat tcgttcctga tcgagagctc
     4321 tttgctg
//
LOCUS       DAORFF010000328         4325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11963_length_4325_cov_3.949649, whole genome shotgun sequence.
ACCESSION   DAORFF010000328 DAORFF010000000
VERSION     DAORFF010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11963_length_4325_cov_3.949649"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKK76_12805"
     CDS             <1..454
                     /locus_tag="PKK76_12805"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNL69404.1"
                     /translation="MHFFAGLRFHPEESKIFRGNDFVYPLHKFIVTSSYGTRLDPFTN
                     KQTFHGGMDLAAPEGTPVLASRPGKVVFCGNLGGYGNLVVLDHGHGYRTLYGHLKAFT
                     VKRGTMVAAGAQIATVGSTGRSTGPHLHFEMRKDGQLARPRLVHDTTY"
     gene            complement(503..2611)
                     /locus_tag="PKK76_12810"
     CDS             complement(503..2611)
                     /locus_tag="PKK76_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNL69405.1"
                     /translation="MLEEIRKNLRNADTVLGIGVILIFALIVVPLPGPLLDALIAVSL
                     LAGLLILLTALSSKGPADFTVFPTILLVTTIYRLAVNVSTTRMILSKGEASNSALVNA
                     FGTFVVGGSGMGSYVIGAVIFIIVMLVQIMVITKGATRVSEVAARFALDAMTGKQMAI
                     DTDLQNGHITEQEARQRRENVQKESNFYGAMDGASKFIQGDVRVGLIITGINIVGGLI
                     IGMTIRGEPFIDALARYTKFTIGDGLVNQIPALMISAATGVIVTRSVGEESLPTDIRK
                     QLFRNPQILYVVGAVLVMAGLLPGFPLLSMGTVGGFILFLGYRMDQSLKQAKIEDVRK
                     EKEKVEERKPESYTQQAKTDKLGVEIGYNLIPLVDPKSGGTLLDQISRLRGQFARDMG
                     LIVPPVRIRDNMSLQPNQYHVRLDGDVVGDFVLKPDHLIGLPTRVRDPLPGLEEFTEP
                     TYNMRAVWVPSDMKTDAENAGYDVVDPASVIATHLSSIIRKSSADIMGRQEIKEILDS
                     IRQDHPVIVSEVLDERKIPMGRIQEILKLLLREGVSIRNMVRILEVIAGQAEKQIYDP
                     YIVSEAVRMALRRQIVADYIQDGVLHCITVSPDIERRMRDAIHSDPTEGFILALKPDL
                     NIAIRDAFVQEFQASQQKGKMPVFMSSRVIRSPLFDYLIRFLPAGNFTVLAHEEVPQG
                     AGIKVEVSGQVALQKAEVAV"
     gene            complement(2611..3822)
                     /locus_tag="PKK76_12815"
     CDS             complement(2611..3822)
                     /locus_tag="PKK76_12815"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNL69406.1"
                     /translation="MKMVHESDLDLSDPIEFEFSASLSFDFAGVDAVFSIPADALPHF
                     HLSRFAAEDEGRTEAPTERRLREEREKGRVARSQDLTNAVVLLGAVMGLYFTGGYMVN
                     QCMQLFHRFFSESAFAQTSFQAEDLKSFVGTLFFEVFKICMPLFGISMFMGIVGNVVQ
                     VGGLFTVRPLFMPERLTPDFTRIIPSRRNFYLLLKTLVLVGIIGFTAYMVIMDDFIPM
                     LKSAGMDTRQALVLFAHSAFKLLIVCAVLLLALAIPDYFYERFEYMENLKMDISEVKR
                     ERKEDFGDPLVRQRQRDRYFQLTRQRNMLKEVPKADVVITNPTHYAVALLYDPAVATG
                     PRVIAKGQDWLAFEIKRIARENDVPIEENPEVARAIYSAVEVGREIPENLYRAVSLIF
                     AKLDKFKAVRS"
     gene            complement(3819..>4325)
                     /locus_tag="PKK76_12820"
     CDS             complement(3819..>4325)
                     /locus_tag="PKK76_12820"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNL69407.1"
                     /translation="GLSFAEVLDPASQTTMPIIGTLKSSIGLLLFLSLNFPVDGITAP
                     AYLHMVRALAFSIKAAPTMMLDMTTLGGLLSTVDQTMGVLFVTALKIGIPAAGILFIA
                     TVALGLIGRAAPQLNLMNMGVHINIILGLTLLLVLSPIYIPIMGGAFQSMYDIIGTML
                     RDWPGKHS"
BASE COUNT         1111 a         1111 c         1040 g         1063 t
ORIGIN      
        1 gatgcatttc ttcgcaggcc tgaggtttca tccggaggag agcaagatct ttcgggggaa
       61 tgattttgtt tatcctcttc acaagttcat tgtaacctct tcgtacggaa cacgactcga
      121 tccattcaca aacaaacaaa cttttcacgg aggaatggac ctggcggcac cggaagggac
      181 accggttctg gcgagtcgcc cgggcaaagt tgtgttttgt ggaaacctcg gaggctacgg
      241 caatctggtt gtgctagacc acggccacgg ttacagaacg ctatacggtc acctaaaagc
      301 attcacggtg aagagaggaa cgatggttgc ggccggggcg cagattgcga cggttggatc
      361 tactggacga tccaccggac cacatttgca ttttgaaatg cgaaaggatg gacagctcgc
      421 gcgcccaaga ctggtgcacg acactaccta ctgatcatac cctctagcag tgcttgccag
      481 cactgcctat cggcactgcc cgttagacgg caacctccgc cttctgcagt gcaacctgtc
      541 cgctcacttc tactttgatg ccggcgccct gtggcacttc ttcatgcgca agcactgtga
      601 aatttccggc cggcaagaaa cggatcaaat agtcaaagag cggagaacga atcacgcggc
      661 tggacatgaa cactggcatt tttccttttt gctgcgacgc ctggaactcc tgcacgaaag
      721 catcgcgaat ggcaatgttg agatccggct tgagagcaag aatgaatcct tctgttggat
      781 ctgaatgaat tgcatccctc attcttcgtt caatgtcagg agaaacagta atgcaatgga
      841 gcacgccgtc ctggatatag tctgcaacaa tctgacggcg aagagccata cgcactgctt
      901 ctgagacaat gtagggatcg tagatttgct tttctgcctg acccgcgatc acttcgagaa
      961 tgcgcaccat gttcctgatc gatacacctt cgcgaagaag tagtttgaga atttcctgga
     1021 tgcgccccat agggattttt ctttcatcaa gaacttcgct gacgatgact ggatgatcct
     1081 gacgaatgct atccaggatt tccttgattt cctggcgacc catgatgtcg gcactcgatt
     1141 tgcgaatgat actcgaaaga tgcgttgcga tgacggatgc aggatccact acatcatagc
     1201 ctgcattttc agcgtccgtc ttcatgtcag aaggaaccca gactgcgcgc atattgtatg
     1261 tgggctccgt gaattcttcg agaccaggca gtggatctcg cacacgagtt ggaaggccaa
     1321 tgagatgatc tggtttaagt acaaaatcgc caaccacatc cccgtccagg cgaacatggt
     1381 attgattcgg ctgtagactc atgttatccc ggatgcgcac cggaggaact atcaggccca
     1441 tatctcgcgc gaattgtcct cgcagtcgcg atatttgatc cagcaacgta cctcctgatt
     1501 ttggatctac aagtggaatc aagttgtagc caatttcgac gcccagttta tccgtcttcg
     1561 cctgctgagt gtaagactct ggctttcgtt cttctacttt ctctttttcc tttcttacat
     1621 cttctatttt agcctgcttg agagactgat ccattctata tccaaggaat agaatgaaac
     1681 cacccaccgt tcccatagat agaagaggga atccaggaag aagtccggcc atgacgagta
     1741 ctgcacccac cacatacaga atctgcgggt tgcgaaacaa ttgcttgcga atgtctgtag
     1801 gtagactctc ttcacccacg cttcgcgtaa cgatcacacc cgtggcggca gaaatcatca
     1861 gagctggaat ctgattcacg agaccgtcac cgattgtaaa ctttgtatac cttgcgagtg
     1921 cgtcgatgaa cggctctccg cgaatggtca taccaatgat caaaccaccc acgatgttga
     1981 tgcctgtgat gatcagacct acccgtacgt ccccctggat gaacttggaa gcaccatcca
     2041 tggctccata gaagttcgat tccttttgaa cattctcccg tctctgtcgt gcttcctgtt
     2101 ctgtgatgtg accattctgc aaatccgtat caatggccat ttgcttgccc gtcatcgcgt
     2161 cgagagcaaa ccttgctgcc acttcggaca cgcgcgttgc accttttgta atgaccatga
     2221 tctgcactaa catcacaatg atgaaaatca cagcgccaat cacgtaactg cccatgccgc
     2281 tgccgcccac cacgaacgtt ccaaacgcat ttacaagtgc agagttagac gcctctcctt
     2341 tggaaagaat catcctggtt gtggacacgt ttacagcaag gcgatagatc gtcgtaacaa
     2401 gtagaatggt tggaaaaaca gtaaagtcgg ccggcccctt ggaagaaagg gcggttagca
     2461 aaatcaacag acctgcaagc aaactaactg cgatcagagc atcgagaagc ggacccggaa
     2521 gtggaactac aatcaatgcg aaaatgagaa tgactccgat gccaagcacc gtgtctgcgt
     2581 ttctcaaatt cttgcgaatt tcttccaaca tcagcttctt accgccttga atttatccag
     2641 tttcgcaaag atcagactga cagcccggta gaggttttcc ggtatttccc gtcccacttc
     2701 cacagcgctg tatatggcgc gcgccacttc agggttttct tctataggta catcgttttc
     2761 gcgtgcgatt cttttgattt cgaatgcaag ccagtcctgc cctttcgcaa tcactcgtgg
     2821 tccagtggca actgcaggat cgtatagaag agcaacagca tagtgagtcg gatttgtgat
     2881 gaccacatcc gcttttggta cttcttttag catgttgcgc tgtcgcgtga gctggaagta
     2941 acgatctctt tgtctctggc gcacaagtgg atcaccaaag tcttcctttc tctctcgctt
     3001 cacttctgaa atgtccattt tcagattttc catatactcg aagcgttcgt aaaaatagtc
     3061 aggaatcgct agtgcaagta gaagaactgc gcagacaatc aagagtttga atgccgaatg
     3121 cgcgaagagt acaagcgcct gtcttgtatc catcccggca gacttcagca ttggaatgaa
     3181 atcgtccatg atcaccatgt acgcggtaaa tccaatgatg cctaccagca ccagagtctt
     3241 gagcaaaaga taaaagttcc ttctgcttgg aatgatgcgc gtgaaatcag gtgtgagtct
     3301 ttccggcata aacagggggc gaaccgtgaa aagaccgcct acctggacta catttccaac
     3361 aattcccatg aacatagaaa tgccgaataa cggcatgcag atcttaaata cttcaaagaa
     3421 cagcgttcca acaaatgact tcaagtcttc tgcttgaaag ctggtttgtg cgaatgctga
     3481 ttcactgaag aatctgtgga acaactgcat gcactgattt accatgtagc cgccggtaaa
     3541 ataaagaccc atcaccgcac cgagcaatac aaccgcattg gtcaaatcct gcgaacgcgc
     3601 gactcgacct ttctctcttt cttctcgcag gcgtctttct gttggagctt cggttcggcc
     3661 ttcatcttcc gccgcgaatc ggcttagatg aaagtgggga agggcgtctg ccggaatgga
     3721 gaagaccgcg tccactcctg cgaaatcaaa cgaaagagaa gccgaaaact caaactcaat
     3781 tggatcagag agatccaggt cagactcatg caccattttc atgaatgttt cccgggccaa
     3841 tcgcgcagca tcgtgccaat gatatcatac atggactgga atgctccgcc catgatcgga
     3901 atgtagatcg gcgaaagaac gagaagcagt gtcaggccca ggatgatatt gatgtgaaca
     3961 cccatgttca tcaggtttaa ttgcggcgcg gcgcgtccga tcaatccaag tgccacagtg
     4021 gcgatgaata gaattcctgc tgcggggatt ccgatcttga gcgccgtgac aaagagaact
     4081 cccatggttt gatccacggt tgacagaagt ccaccgagag tggtcatatc gagcatcatg
     4141 gtgggcgcag ccttgatcga aaacgcaagt gcgcgaacca tatggagata ggcaggcgct
     4201 gtgatcccat ccactggaaa gttcaggctg agaaaaagca aaaggccgat gctgcttttg
     4261 agagtgccaa tgattggcat ggtggtctgc gatgcaggat ccaggacttc tgcgaaagac
     4321 aatcc
//
LOCUS       DAORFF010000329         4320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_11994_length_4320_cov_4.026729, whole genome shotgun sequence.
ACCESSION   DAORFF010000329 DAORFF010000000
VERSION     DAORFF010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11994_length_4320_cov_4.026729"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="PKK76_12825"
     CDS             <1..1304
                     /locus_tag="PKK76_12825"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNL69408.1"
                     /translation="VDVYKLKLDEQEDQYMFDGQLRPLNKTSVRIQVDIGLFDLPIPM
                     STYESAQGPVLKSDHGKYAVRVSGIDRLIFAGEQWYRMNKARNLTEWKKAMQMQAIPM
                     FNAVYADASNIFYVYNASLPTGRVPNQSYRKVLPGDTSQNIWDDKILPFAKLPAVENP
                     PSGFIQNCNSSPFETTTGPGNPDPGRYSLSMGIEKRSTNRSMRSRELLSKPGKISFED
                     FLRMKWDRTYAPRSPIYEYAINPLLRDFKPQNEDESQALELLRKWNGRADDSPGSSLA
                     ILVYRPIWQQTDLDHGPIPDRAILLRDAISFLKKHYGKLDVPLSILQRMRHGHLDEGL
                     VGGPDVLNAAHSLEEKDHIVGVAGDSYVLIVQFQPANVKSWSIHQYGNSTHKGSAHFA
                     DQMPLFLKRTLKSTFRDVRDLNEHTECQYHPGGQSCAEARD"
     gene            complement(1310..2299)
                     /locus_tag="PKK76_12830"
     CDS             complement(1310..2299)
                     /locus_tag="PKK76_12830"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNL69409.1"
                     /translation="MKFRVLSSLRSFSRSTSFTLAGGVAFLLVMAGTSFFVQKVFAQV
                     PAPRFREATVLVPRGSIYYKVMGKGQPIILVHGGPGLDHRYMFQAFQDLAKDYRVIFY
                     DQRGTGASQCKLSDRSVTIEQFVEDLDAVRRHLGLRRINLLGHSWGGLLAVHYTVRHP
                     QNVRSLMLANSSGLTGESQKAFFENLDSMRTPEDKEELENLKGKSDQESLQRHNELYM
                     RPYFYDSSKIALLSYEPSKLTAENNERVRTLIWRQLLDYDLRPQASRLHIPTLVIHSD
                     RDVIPLEVAEETAETIPRSELVVMQDVGHYSFIEDPTEFFKEIRKFLKTVHGP"
     gene            complement(2296..2829)
                     /locus_tag="PKK76_12835"
     CDS             complement(2296..2829)
                     /locus_tag="PKK76_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNL69410.1"
                     /translation="MIQFQASNFDTVKTRLESLGATNIHHDGASVVAEIPRTKAFDII
                     KKIKDDPEIGFRFFTDITGADFLKYSVKKPERYAVIYVLLSPSLGVRLELIAYLPEND
                     PSIQSIASLFTGANWAERETYDMYGFEFKGHPNPGRLLTPEGFEGHALRKDYPLKGKG
                     ERSSFPVYRATPGHASE"
     gene            complement(2831..3325)
                     /gene="nuoB"
                     /locus_tag="PKK76_12840"
     CDS             complement(2831..3325)
                     /gene="nuoB"
                     /locus_tag="PKK76_12840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNL69411.1"
                     /translation="MGLNPSTDFATSRIDAIVNWSRANSLWPMPFGVACCAIEMMASY
                     MPKYDTSRFGAEVLKFSPRQSDLLIVSGRISLKMMPVLTRIWEQMPEPKWCISMGACA
                     SSGGVFDTYALIQGIDQFIPVDAYIPGCPPRPEAFIDAVMMIQEKAKKGQARSQDYVD
                     RAVV"
     gene            complement(3316..3681)
                     /gene="ndhC"
                     /locus_tag="PKK76_12845"
     CDS             complement(3316..3681)
                     /gene="ndhC"
                     /locus_tag="PKK76_12845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNL69412.1"
                     /translation="MNPVLEQYWPFAVFFVVAMLVAVGGLVVQSFLGPKLSSKHKFDP
                     YECGLDQMDSPHKPYSLKFYVVALLFMLFDIETIFLLPWAVGYRSFGWESLIAVIIFS
                     GIVVFGLWYVVKLKVFEWD"
     gene            complement(3732..>4320)
                     /locus_tag="PKK76_12850"
     CDS             complement(3732..>4320)
                     /locus_tag="PKK76_12850"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL69413.1"
                     /translation="RIILGSDGRDDLILPAEDGTTRMNEDSELFCSVVQSAQGDLPRI
                     REMLGSTGEIIDDLSLACVSILQLRERMSKRAKKITLQDQIKVKNWKEGAVTALSLLE
                     DDPADLDFFPVAARICQKAGIVSQAIDLLESYWLRRPQQSPSRLRLATLYHQIGKHDR
                     ASAILSGYREAFPDDPKGAALSTLLGSARVGPGAS"
BASE COUNT         1136 a         1115 c         1055 g         1014 t
ORIGIN      
        1 ttgtggatgt ttacaaactc aaactcgacg aacaagaaga tcaatacatg ttcgatgggc
       61 agctgcgtcc tctgaacaaa acgagcgttc gcatccaggt cgatatcgga ctttttgatc
      121 tacccatccc catgtccacg tatgagtcgg cacagggccc cgtgctcaag tccgatcatg
      181 gaaagtatgc tgttcgagtc tctggcatcg atcgactcat ctttgcaggc gaacaatggt
      241 atcgcatgaa caaagcgcgc aatctcaccg aatggaaaaa agcgatgcag atgcaagcaa
      301 tcccaatgtt caatgctgtg tacgcggatg cttccaatat tttctatgtc tacaacgcgt
      361 cattgcccac cggcagagtc ccgaatcaat cgtatcgcaa agttctcccg ggagacacaa
      421 gtcagaacat ctgggatgat aaaatcctgc ctttcgcaaa actccctgcc gtagaaaatc
      481 ctccttccgg attcattcaa aactgcaact cgtcgccatt tgaaacaacg acaggcccag
      541 ggaatcctga tccgggacgt tattcgctat ccatgggaat tgagaagaga agcaccaatc
      601 gttccatgcg ttcgcgcgaa ctactttcga agccgggcaa gatcagtttc gaagatttct
      661 tgcgaatgaa atgggacagg acctatgctc cccgatcgcc tatctacgag tatgcgatca
      721 acccgttgct tcgagacttc aaaccgcaga acgaagatga atcccaggcc ctcgaattgc
      781 ttagaaagtg gaacggaagg gcggatgatt ctcccgggtc ctctcttgcg attctggttt
      841 atagacccat ctggcagcaa acggatttag atcacggtcc catcccggat cgagccattc
      901 tactgagaga tgcgatttca tttctaaaga agcactatgg aaaactggac gtacctctct
      961 ctattttgca gagaatgcga cacggccatc tggacgaagg gctggtgggt ggcccggatg
     1021 tgctcaatgc cgcccattcg cttgaagaaa aagatcacat cgtgggagtt gccggggatt
     1081 cttatgtgct catcgttcaa tttcagccag cgaatgtgaa atcctggtct atccatcagt
     1141 acggaaattc aacgcacaaa ggttcggcac atttcgcaga ccagatgcct ctgttcctca
     1201 agcgaactct caaatctact tttcgcgatg tacgcgatct aaacgagcat acagaatgtc
     1261 agtaccatcc aggaggccag agctgcgcgg aagctcgtga ttaggccgat cacggaccgt
     1321 gaacggtctt caggaatttg cggatctctt tgaaaaactc agttgggtct tcaatgaaac
     1381 tgtagtgtcc cacatcctgc atcactacca actcggaacg cggaattgtc tccgccgttt
     1441 cttctgcgac ttcaagagga atcacatctc gatccgagtg aatcactaga gtggggatgt
     1501 ggagacgcga agcctgcgga cgaaggtcgt agtccagaag ctgacgccag atcaaagttc
     1561 tcactcgctc attgttttca gcggttagtt tgcttggttc ataggacaag agggcaattt
     1621 tcgaagagtc atagaaatag ggccgcatgt acaattcgtt gtgtctctgg agagattcct
     1681 ggtctgactt tcctttcagg ttctccagct cctctttgtc ttccggtgtt cgcatgctat
     1741 ccagattctc aaaaaatgcc ttctgcgatt ccccggtcag gccggatgaa ttcgcaagca
     1801 tgagagatct tacattctgc ggatgcctca cagtgtagtg aaccgccagc agtccacccc
     1861 aggaatgacc cagcaaatta atacgacgaa gcccgaggtg cctgcgaacc gcatcgagat
     1921 cttccacaaa ctgctctatg gttacagatc gatccgagag tttgcattgt gatgcgccag
     1981 ttcctcgctg atcatagaag atcacgcggt aatctttcgc gagatcctga aatgcctgaa
     2041 acatgtagcg atgatccagc cccgggccgc cgtgaacgag tatgattggc tggcctttgc
     2101 ccattacctt gtagtagatg gatcccctcg gaaccaggac cgtggcttcg cgaaaacgcg
     2161 gggccggcac ctgtgcgaaa actttttgca caaagaagct cgttccagcc atcacaagga
     2221 ggaacgcgac tccccccgcg agagtgaagg aagtggatct ggagaacgaa cgcaggcttg
     2281 ataaaactct gaacttcatt cggacgcgtg accgggagta gcccgataaa cggggaagct
     2341 ggacctttcg cctttgcctt tcagtggata atctttgcgc agtgcatggc cttcgaaccc
     2401 ttccggagta agaagcctgc cggggttcgg gtgtcccttg aattcaaagc cgtacatatc
     2461 gtatgtttcg cgttctgccc agttggcgcc agtgaacaga gatgcgatgc tctggatgct
     2521 tggatcgttt tcgggtagat atgcgattag ctcaaggcgc acgccaagag atggagacaa
     2581 aagaacatag atgaccgcat aacgttccgg tttcttcaca gaatacttca ggaaatcggc
     2641 acctgttata tccgtgaaaa agcgaaatcc tatttccgga tcatctttga tcttcttaat
     2701 aatatcgaat gcttttgtcc gcggaatttc tgcaaccacg gaagcaccgt catgatgaat
     2761 gttcgttgcg ccgagagact caagtctggt ctttacagtg tcaaaattcg aggcttgaaa
     2821 ctgaatcatc tcaaacgacc gcccgatcca cgtagtcctg agaacgggcc tggccttttt
     2881 ttgctttttc ctgaatcatc atcacagcat caatgaaagc ttccggcctg ggtggacatc
     2941 caggaatgta tgcatccaca ggaatgaatt gatcaattcc ctggatcaga gcatacgtat
     3001 caaacactcc gccactggac gcgcatgcgc ccatagaaat gcaccacttt ggttcaggca
     3061 tctgttccca gatgcgagtg agcactggca tcatcttgag agaaatgcgg ccggagacaa
     3121 tgagcaaatc agactgcctg ggagagaatt tcaaaacctc cgcaccaaat cgcgaagtgt
     3181 catactttgg catataagag gccatcatct caatcgcgca gcacgcaaca ccgaatggca
     3241 taggccacag gctgtttgct ctcgaccagt ttacaatcgc atcaatacga gaagtggcga
     3301 aatctgtgct tggattcaat cccattcgaa caccttcagc ttcacaacgt accagagacc
     3361 aaagactacg atgccagaga aaatgattac ggcgatcaag ctctcccacc caaaggaacg
     3421 atagccaaca gcccagggca acaggaaaat cgtttcaata tcgaagagca tgaataagag
     3481 cgcgaccacg taaaacttaa gagaatacgg tttgtgcggt gaatccatct ggtccaggcc
     3541 gcattcatat ggatcgaact tgtgcttgct ggaaagtttt gggccaagaa acgactgaac
     3601 cacgaggcca cccactgcca ccagcatggc gactacgaaa aaaacagcaa aaggccagta
     3661 ctgctcgaga accggattca taggcgcaag ttccttcgaa tcgagggttc tgtaaataga
     3721 aaaagaacag tttagcttgc gccggggcca accctggcgg acccgagcaa tgtactgagt
     3781 gcagcgccct ttggatcgtc gggaaaggcc tccctgtatc cagaaaggat cgcagatgca
     3841 cgatcatgtt tgccaatctg atgatagaga gtcgcaagcc tgagcctgct gggcgattgc
     3901 tgaggccttc tgagccagta actctccaac aagtcgattg cttgcgaaac aatgcccgcc
     3961 ttctgacaaa ttcgcgctgc caccgggaaa aaatcaagat ctgctggatc gtcctcaaga
     4021 aggctcagag cggtcactgc tccctctttc cagttcttca ccttgatttg atcttgcagg
     4081 gtgatctttt ttgcacgttt ggacattcgc tctcgcaatt ggagaatgga aacacatgcg
     4141 agcgaaagat catctatgat ttcgccggtg cttccgagca tctcgcgaat ccgaggtaga
     4201 tcaccctgag cggactggac tacgctacag aataattctg aatcttcgtt cattctggtg
     4261 gtgccatctt ctgcgggcaa aatcaaatcg tcgcgaccgt ccgatccaag gatgatgcga
//
LOCUS       DAORFF010000330         4313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12029_length_4313_cov_3.562940, whole genome shotgun sequence.
ACCESSION   DAORFF010000330 DAORFF010000000
VERSION     DAORFF010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12029_length_4313_cov_3.562940"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PKK76_12855"
     CDS             complement(<1..423)
                     /locus_tag="PKK76_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69414.1"
                     /translation="MINAVFKAFTVLGSEWVLVILLLLSVLSIAMTLERWQFYRKAQS
                     GIVAFRSSLRTLIVEAKWKESLALVEARIAAKKGKYPDLETDMAYTLLTHTKAAGGQA
                     VSPASMNQLAQDSLTRSRIAWDKYLTVLASIGSNTPFFG"
     gene            521..1297
                     /gene="lpxA"
                     /locus_tag="PKK76_12860"
     CDS             521..1297
                     /gene="lpxA"
                     /locus_tag="PKK76_12860"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNL69415.1"
                     /translation="MKIHPTAIIHPTAKLHESVEVGPFCIIDQDVVMGEGCFVESTVK
                     IHARTVIGKNNRFHHGAIIGCEPQDLGYDRSSPCYVKIGDNNVFKEGANIHRGTKPDH
                     PTLIGNNNYFMGNFHVGHDCILGDHSIFTHGTVLAGHVTVGNYAFISGVAAVHQFCKV
                     GDYSMIAGCSKIVKDVPPYATADGNPATLIGINSVGLKRAGYSPAKRDAIKQAYKLLF
                     HSGKNIKQALAELKSQENTDEVKLIVKFFEDSDRGVTDHR"
     gene            1294..1860
                     /locus_tag="PKK76_12865"
     CDS             1294..1860
                     /locus_tag="PKK76_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69416.1"
                     /translation="MKRFAVLLLLFPAILIAEDVKAKLGIELKRGTASSKAHARETVR
                     SGDRITAYVLPAQNSNVYVVSIKDGKASLINGKGIALSSSEAAAIPSMESPIILTEKG
                     LVEIQIYVSREPISDLNELANGVTVSRFREIERRIKDRAGITGAPEKPAPVAANVRAM
                     GADVIAREMPGYSSPKIILAKFYFDVQN"
     gene            1847..3880
                     /locus_tag="PKK76_12870"
     CDS             1847..3880
                     /locus_tag="PKK76_12870"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL69417.1"
                     /translation="MFKTEPDRSASLILSSLWTILGLLLIAVIGTWHRTDERLNDWIA
                     RVAVHRGFAPASSPRICYLFITDTTYARFNKQALNREQLAHANDILADLGAESIVYDL
                     ILARPGEQSADAAFARSLERTKIVFLPSGFAARKRKPVDPDALEVRESGRGGAPIALH
                     EIASLPDFAQRARGGGHVFAPSDSDGVYRRMNLFVQEGRRIIPGLALSVLLFQRHQTL
                     ADIEIHWEDEVRIPATASAPELIIPIDSAGATRIAYTAKWGRDFESMTIDDLIAGYED
                     PDKRGNLINMFEGRTVFIGDTSTGIADAGATPLENDVPLVAMHAALLSSMMDQHSFGV
                     WKPVHVWALFTACCILLVFSSTLRRAGSLHITAVLLLIALWILPFLGYYRYTLFPGAT
                     VLAVFVLVYLGTTVSIQVRVWKQNVIIRAENSFLHRDLSIAANIQSKMLPAALPDRSD
                     LEIAAVNIQAQSIGGDFYDVITLPSGKVLLLAGDVSGKGIPAALNMSGILSSFRTIVA
                     TGAASTPREILLHLNDVLIKQIRPGELTSFATAVLLLIDPVEMSVQMARCGHELPIFW
                     NPGNREMREIKTSGFMLGVSPSSMMSGVLNETSVQLARGDAIYIYSDGVTEAANVDGE
                     FYGLSRLKSAIRAFGSGPAQELLDVLLSDIRAFSGIAPQHDDITLVIVRLLGP"
     gene            3976..4313
                     /locus_tag="PKK76_12875"
                     /pseudo
     CDS             3976..4313
                     /locus_tag="PKK76_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020781780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
BASE COUNT         1104 a         1107 c         1074 g         1028 t
ORIGIN      
        1 tccaaagaat ggcgtgttac ttccaataga agccagaacg gtgagatact tatcccatgc
       61 gatacgcgat ctggtcagag aatcttgcgc tagttgattc atggacgcag gcgaaaccgc
      121 ctggccaccg gctgctttgg tgtgagtcag caaagtgtaa gccatgtccg tttcgagatc
      181 tggatactta cctttcttgg ctgcaatccg ggcctctacg agtgcaagag attccttcca
      241 cttggcctca acaatcaatg tgcgaaggct ggatcggaaa gccacaatgc ccgactgggc
      301 tttgcgataa aactgccacc tttccagagt catcgcaatg gaaagaacag atagtagcaa
      361 cagaatgacc agaacccact cagatcccag tactgtgaag gctttgaaaa ctgcgttaat
      421 catatcagat gtgaccagga aaacagctgg agctggcctg gtctactttg aaatttggtt
      481 gccctgcatt ccagggcacc tccagatacc aggataagat atgaagattc acccaactgc
      541 gatcattcac ccaactgcaa aacttcacga aagcgtggag gtgggaccat tctgcatcat
      601 agaccaggac gtggtcatgg gcgaaggctg cttcgtagaa agcaccgtaa aaatccacgc
      661 tcgcaccgtc atagggaaga acaaccgttt ccatcatggt gccatcattg gctgcgagcc
      721 gcaggacctt ggatacgatc gctcctctcc ctgctatgta aaaatcgggg ataacaacgt
      781 ttttaaagaa ggagccaaca tccaccgggg cacgaaaccg gatcacccga ctctaattgg
      841 aaacaacaac tacttcatgg gcaattttca tgtcggccat gattgcatcc tgggagacca
      901 cagcatcttc actcacggca ccgtactggc cggtcatgta acggtcggaa actacgcttt
      961 catttccggt gtggccgcag ttcaccagtt ctgcaaagtg ggggactatt ccatgatcgc
     1021 tggatgttcc aagattgtga aagatgttcc gccttacgcc acagcagatg gaaaccctgc
     1081 cacgctgatt ggaatcaaca gtgtcggtct caaacgcgcc ggctattctc cagccaaacg
     1141 cgacgcgatc aaacaggctt acaaattgct ttttcattcc ggcaagaata tcaagcaagc
     1201 gctcgctgaa ttgaagagcc aggaaaacac agacgaagta aagctgatcg tgaagttctt
     1261 tgaggacagc gatcgcgggg tcacggacca cagatgaaac gattcgcggt gttactcctc
     1321 ctgtttcctg cgatcctgat cgcagaagac gtcaaagcaa aactcggaat cgaactcaaa
     1381 cgtggcactg cttcttccaa agctcacgcc agagaaaccg tacgctcagg tgatcgcata
     1441 acggcatatg ttctccctgc tcagaactca aatgtatacg tagtttcaat caaagatgga
     1501 aaagcatctt tgatcaatgg aaaggggatt gcgctttctt cctcggaagc ggccgccatt
     1561 ccatctatgg aatcgccaat cattctgaca gaaaagggtc ttgtagaaat tcaaatctat
     1621 gtttctcgcg aaccgatctc tgacctaaac gagctagcaa acggagtcac cgtgagtcgc
     1681 tttcgcgaaa tcgaacgaag aatcaaggat agagcgggaa tcacaggagc accagaaaaa
     1741 ccagctccgg tggcggctaa cgttcgagcc atgggagcag acgtgattgc acgggaaatg
     1801 ccgggctact ccagcccgaa aatcattctg gcaaaattct atttcgatgt tcaaaactga
     1861 gccggatcga tccgcgtctc taattctctc ctcgctctgg accatacttg gcctgcttct
     1921 cattgcagtc attggaacct ggcatcgcac ggatgaaaga ctgaacgatt ggattgcgag
     1981 agttgcggtt catcgtggtt ttgctcctgc ttcttcgcct cgcatatgtt acttgttcat
     2041 cacggataca acgtacgcgc gcttcaacaa gcaggccctc aatcgcgagc aacttgctca
     2101 cgcaaacgac atactcgcag acctgggcgc tgaatcaatc gtatacgatc tcatcctggc
     2161 aagaccggga gagcagagtg cggacgcagc tttcgcacgc tcattggaaa gaacaaagat
     2221 cgtatttctt ccgtctggat tcgccgcacg caagagaaaa cctgtagatc ctgatgccct
     2281 ggaagtgcgg gaaagcggaa gaggtggtgc cccgattgcg cttcatgaaa ttgcatcgct
     2341 tcctgacttc gcgcaaaggg cccgcggcgg cggacatgtt tttgcgccgt cggattcaga
     2401 cggtgtttat agaagaatga atctgttcgt ccaggaaggc cgccggatca ttccggggct
     2461 ggctctttct gtccttcttt ttcagagaca tcaaactctc gcggacattg aaatacactg
     2521 ggaagatgaa gtccgaattc ctgcgactgc gagcgcacca gagctcataa ttccaattga
     2581 ttccgccggc gctacacgaa tcgcctacac cgcgaaatgg ggacgcgatt ttgaaagcat
     2641 gacgatagac gatctgatcg ccggatacga agatccagat aagcgcggca atttgatcaa
     2701 catgttcgaa ggcagaactg tcttcattgg agacacaagc actggcattg cagatgcagg
     2761 cgccactcca ctcgaaaacg atgtgccact ggtggccatg catgcggctt tactgagctc
     2821 catgatggac caacattcat tcggtgtgtg gaagccagtg catgtgtggg ctctttttac
     2881 tgcttgctgt attcttcttg ttttttcctc cactttgaga cgtgcgggtt ctttgcacat
     2941 aacagctgtt ttactcctta tcgcactctg gatacttcca ttcctgggat actaccgtta
     3001 tacgctcttt cctggcgcca ccgtgcttgc cgtgttcgtt cttgtatatc tgggtacaac
     3061 ggtcagcatt caggtgcgcg tctggaaaca aaatgtaatc attcgggcag aaaatagttt
     3121 tttgcataga gacctgtcga tcgcagcaaa catacagagt aagatgctcc cggccgctct
     3181 cccggatcga tcggatctgg aaattgctgc agtaaacatt caggcgcaga gtattggcgg
     3241 cgatttctac gatgtgatca ctctgccatc cgggaaggtg ctgctcctcg caggagatgt
     3301 ctccggaaag ggaattccag cagcgctcaa catgagcgga atactcagta gctttcgcac
     3361 cattgttgct acgggagcgg cctccactcc acgcgaaatc ctgctccact tgaatgacgt
     3421 cctgatcaaa caaattcgtc cgggggaact cacttcattc gcgacagccg tcttgcttct
     3481 gatcgatcct gtggaaatga gcgttcaaat ggccaggtgc ggacatgaac tcccgatctt
     3541 ctggaatccg ggcaatcggg aaatgagaga aataaaaaca tcgggcttca tgctcggagt
     3601 gtctccttct tcgatgatgt ccggagtgct caatgaaact tcagtgcaat tggctcgcgg
     3661 agatgcaatc tacatctatt cggacggagt gacggaagca gccaacgtgg acggagaatt
     3721 ttacggtctt tccagattga aatcggctat ccgcgcattc ggcagcggac ctgcacagga
     3781 gttgctcgac gttcttctat ctgacattcg tgcattttct ggaatagctc cacagcatga
     3841 cgacattacg ctggtgatcg tgcgcttgct cggcccgtag aaaaaaacct ggacggattc
     3901 gcgcaaacag gacggcatgc tcctgtgaga atcctgcttc tgttagtttc atttactttt
     3961 gcattttgtg ggagaaaaga gaacgccctt ctcaatgtat cttacgatcc cacgcgagag
     4021 ttcttccatg aatacaacga gctgtttcaa actcactgga agaaagtgag tggtgaagac
     4081 attcaggtga ctcagtcgca cggcggttca ggaaaacagg ctcgcgccgt gattgatgga
     4141 ctccctgcag atgtagttac gttaggatgt tcctacgaca tagacagaat cgccgcgcga
     4201 ggactccttc cagcggactg gcaacaaaaa ctcccgaaca actcttctcc tttttattcc
     4261 acgattgtgt ttgtagtgag gaaaggaaat ccgcggaaca ttcatgactg gcc
//
LOCUS       DAORFF010000331         4304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12071_length_4304_cov_3.765357, whole genome shotgun sequence.
ACCESSION   DAORFF010000331 DAORFF010000000
VERSION     DAORFF010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12071_length_4304_cov_3.765357"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..1749
                     /locus_tag="PKK76_12880"
     CDS             37..1749
                     /locus_tag="PKK76_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL69418.1"
                     /translation="MSNRAFASVADVRTVICIAYAAQPAALGFFVAFLNAYTGAGFRK
                     TTRLMIAFDSILAIAALSLPYPYLQSWLIVFYMVMAPQLILVLYIIGAVVKSRAPGSA
                     FAAASISLGLGFVVWDILDTVFFRTSIRLSRYAHFPVILAMMLMLVNRSLASMEEVEK
                     LNLELSFATKDLDQRVQERTRQLEAAMSSAKRADRAKTSFLANMSHEIRTPLNVLIGM
                     GDWLHEREEDSEKRRVLASMLTAGNGLLTLIQELLDVARIESGTIRLDSVPFDAEAIA
                     QDVVHFFEGRAKEKSLSLEYQSPAKRIGPLRGDPARIRQVLVNLLGNSLKFTETGSVR
                     LTLTLLSETSDTSKIRFLVKDTGPGIAPEDTRTIFERFAQGVSSMNRKHAGTGLGLSI
                     SQDLLKLMGSKIDLTTTVGHGSEFAFTLDLPKSADVIAEIVHSPELPDERPLRILVAE
                     DDEVNRTLLSMYFAQSAHTLEFAHNGLEAVRAFESHPFDVIFMDLMMPEMDGIEATIR
                     IRKIETELLSSGVRTQKTPILALSASTYAEDVALSKQAGCDEHLAKPVKRATLFAALA
                     RVTS"
     gene            complement(1805..2887)
                     /locus_tag="PKK76_12885"
     CDS             complement(1805..2887)
                     /locus_tag="PKK76_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     response regulator"
                     /protein_id="HNL69419.1"
                     /translation="MKQQSDLIETQVENTDQSRFEILVVEDDEIQSRLLREFLEILRY
                     SVREAPTCADALRLIHERRPDLTLLDVHLPDGSGLDLVQQIHLIDKDASVVLMSADRT
                     AEMLVESLAKGAEEFLEKPLDLGELAIRIQNLLQTVAFRKKSEELQAELAREKEILTR
                     YFSAETVTSILTGEMEADASGQTGVATILIFDLRNSTGLAEIMGAIPFAELLNSLMIA
                     LMDLIFEKNGSIAKLTGDGLVATFLPGQACAAISCAQSVAQYFQSIAQESSSEPARKL
                     GFGIGIATGKVFQGNVGTFRRLEWTIVGDAVARAARLESLTKKLGCSILIDRPTMRGC
                     GSQETSAREKRVLIKGRKATLYTLAD"
     gene            complement(2895..3965)
                     /locus_tag="PKK76_12890"
     CDS             complement(2895..3965)
                     /locus_tag="PKK76_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     response regulator"
                     /protein_id="HNL69420.1"
                     /translation="MAQLVAVRMESFARVLVVDDDPDYCILIQSFLEKRGYAVSSLLE
                     YKELANRLQTFRPDAVLLDLNLGDESGINLLPSIRNLCPDAGIVMMSASKSPDLVFHA
                     LFEGASDFLEKPLLPPEVDLRIRGILKKRDFRNGIREATAALEKDKRMLLRYFSRETA
                     EEILSGRLNADMKGSHIDISIMFFSIKNAGVLLRTMDPAAFAAFLNQAIADVIDLVSA
                     NHGSVNKLNGAGLLATFGLPVPRATDSLNAISCARNIRDHFQVANDAGIYESGSLEIG
                     LGIASGLVFAGNIGSVRRMEYTVIGDTANLASRLDALCGESKGDILCDRRTVERSGLK
                     DVTRIADRTIRGRKGEVVIYRV"
     gene            4082..>4304
                     /locus_tag="PKK76_12895"
     CDS             4082..>4304
                     /locus_tag="PKK76_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNL69421.1"
                     /translation="MAHTASGTAQQLHQIIRMNEDVKTILATAEAINLLALNASVTAR
                     RIGSLGRGFVQVASELTEFSRETRRTMNNL"
BASE COUNT         1011 a         1116 c         1052 g         1125 t
ORIGIN      
        1 ttttccagat tgatgcctcc gtaaaaattg gtttgtttgt ccaatcgtgc ttttgcttct
       61 gtggcggatg ttcgcacagt gatttgcatt gcttatgccg cacagcccgc ggctctcgga
      121 ttctttgtag catttctcaa tgcgtacacc ggtgccggat ttagaaaaac gacacgcctc
      181 atgatcgctt ttgattccat tcttgcgatc gccgcactct ccttaccgta tccatacctg
      241 cagagctggc tcatcgtctt ctatatggtg atggcgcccc agttgattct tgtactgtac
      301 atcattggag cggtcgtgaa gagtcgcgcg cccggtagcg cttttgctgc tgcaagtatt
      361 tctcttggtc tcggatttgt tgtatgggat atcctggata cggtcttctt ccgcacaagt
      421 atccgcttgt cgcgatatgc tcattttcct gtgattcttg cgatgatgct gatgctggtg
      481 aatcgatcgc ttgcgtctat ggaagaagtg gagaagttaa atttggagct ttcttttgct
      541 acaaaggatc tggatcaacg cgtgcaagaa cgtacgcgcc agctcgaagc ggccatgagc
      601 tctgcaaagc gcgctgacag agcaaaaaca agtttccttg cgaacatgag ccatgaaatc
      661 agaacgccgc ttaacgttct tattggaatg ggggattggc tgcacgaaag agaagaagat
      721 tctgaaaaac gtcgcgttct tgcaagcatg cttacagccg gcaatggtct tctcactctg
      781 attcaggaat tgctggatgt tgcgcgcata gaatcaggta cgatacgcct tgattcagtg
      841 ccctttgacg cagaggcgat cgcgcaggat gtagttcatt tttttgaagg ccgtgccaaa
      901 gaaaaatcat tgagcctcga gtatcaatcc cccgcgaaaa gaattggacc gcttcgcggc
      961 gatcccgcca ggattcgcca ggttcttgtg aatctacttg gaaactcact aaaatttacc
     1021 gaaacaggaa gtgttcgttt gactctgacc cttctcagtg agacttccga cacctctaag
     1081 attcgctttc tggtgaaaga caccggaccg ggaatcgctc cagaagacac ccggactatt
     1141 tttgaacgtt tcgcgcaggg agtttcctcc atgaacagga aacacgcagg caccggtttg
     1201 ggtttgtcta tcagtcagga cttactgaaa cttatgggca gtaagattga tcttacaaca
     1261 accgttggcc atggttctga attcgcattt actttagatt tgccaaaatc cgcagacgtg
     1321 atcgcagaaa tcgtacattc gccggagttg ccggacgaac gtccgctgcg gatccttgtt
     1381 gcggaagatg atgaagtaaa tcgcacactg ctttccatgt attttgctca gtctgcgcat
     1441 accctggagt tcgcgcataa cgggctggaa gccgttcgtg cctttgaatc acatcctttt
     1501 gatgttatct tcatggactt gatgatgccg gaaatggacg gaatcgaagc taccattcgg
     1561 attcgaaaaa ttgagaccga gcttctttct tctggagtgc gcactcaaaa gactccgatc
     1621 cttgctttgt ccgcatccac atatgcggag gatgttgctc taagtaagca agccggttgc
     1681 gacgagcatc ttgccaagcc ggtgaaacgc gcgacgttat tcgccgctct ggcacgcgtt
     1741 acctcttagt cgcatggatt gaaattcgca atcgagtctg gatgcaagca catgcgaaga
     1801 cccatcaatc cgcaagggta taaagggttg cttttcttcc tttgataagg actcgttttt
     1861 cgcgggcaga agtttcctga gaaccacagc ctctcattgt aggtcgatcg atgagaatgc
     1921 tgcaaccgag cttctttgtc aggctttcca gcctcgcagc ccgcgcaacc gcatccccca
     1981 cgatggtcca ttcaagtctg cgaaaggtgc caacgtttcc ttgaaatact tttccggtag
     2041 caattccaat cccgaatccc agcttgcgcg caggttcgct cgaactttct tgcgcgatgg
     2101 actggaagta ctgcgcaacg ctttgtgcgc acgagatcgc agcgcacgcc tgtcccggaa
     2161 gaaaagttgc aacaaggccg tcgccggtta gttttgcaat gctgccgttc ttctcaaata
     2221 taagatccat gagcgctatc ataaggctgt tgagtagttc cgcaaaggga atagccccca
     2281 ttatttcggc gaggcccgtc gaattacgca aatcgaatat caggatcgtc gcgactcctg
     2341 tttgtccact tgcatctgct tccatttctc ccgtcagaat agatgtcaca gtttccgctg
     2401 agaaataacg cgtgagtatt tccttttctc ttgcgagttc tgcctgaagc tcttcgcttt
     2461 tttttcggaa ggcaaccgtt tgcagcaagt tctgaatacg gattgcgagt tctcccaggt
     2521 ccagcggctt ttcgagaaac tcctccgcac cctttgccag actctccacg agcatctccg
     2581 cagttctatc cgcagacatc aacactaccg aggcgtcttt atcaatgaga tgaatttgtt
     2641 gcaccaggtc caggccggat ccgtccggca ggtgcacatc gagcaaagtc agatcgggcc
     2701 ggcgttcatg gatcaatcgt aacgcatccg cgcacgtggg cgcttcgcgt acggagtagc
     2761 gtagaatttc cagaaactcg cgcagaagtc tggattggat ttcatcatct tcgaccacta
     2821 gaatttcaaa acgagactga tctgtgttct ccacttgcgt ctcgatcaag tctgattgct
     2881 gcttcatccc cgcatcaaac acgatagatc accacttccc cttttctccc gcgaatggtt
     2941 ctgtcagcga ttctggtgac gtccttgagt ccgcttcgtt ccaccgtccg acggtcgcac
     3001 aaaatgtctc ccttgctttc cccacagagc gcatccaggc gactcgcgag gtttgcagtg
     3061 tccccgatta cggtgtactc catgcgacga acactcccaa tgtttccggc gaagacgagc
     3121 ccggatgcaa tgcccagtcc gatttcgagg ctgcctgatt cataaattcc tgcatcgttc
     3181 gcaacttgaa aatgatctct gatatttcgc gcacaggaaa tagcgttgag cgagtccgtc
     3241 gcgcggggca cgggcaatcc gaacgtagcg agtaatcctg caccattcaa tttgtttaca
     3301 gacccgtgat tagcggatac gagatcgatt acatctgcaa ttgcctgatt tagaaaagca
     3361 gcgaaagcgg cgggatccat tgttctcagc aatacaccag cgttcttgat gctaaagaac
     3421 ataatagaaa tatctatgtg agatcctttc atgtctgcgt tcaagcgacc ggaaaggatt
     3481 tcttccgcag tttcgcgcga aaaatatcga agcaacatcc gcttgtcttt ttcgagggca
     3541 gctgtggctt ctcgaattcc gtttctaaaa tctcgttttt tgaggatgcc ccggattcgc
     3601 aaatccactt cgggaggtaa cagcggcttc tccaggaaat cggatgcgcc ttcgaatagc
     3661 gcatggaaga cgagatccgg ggatttgctc gcggacatca taacaattcc cgcgtcggga
     3721 cagagatttc gaatagaagg gaggaggttg attccgcttt cgtcgccaag gttcaaatca
     3781 agcagcaccg cgtccggtcg gaatgtttgc agacggttcg ccagttcctt gtattccaaa
     3841 agcgatgaca cagcgtaacc gcgcttctct agaaagctct gaatgagaat gcaatagtcc
     3901 ggatcatcgt ctactaccag gacgcgcgcg aacgattcca tgcgtacagc gacgagttgt
     3961 gccatgctat tgtaactctt tttttttcgc aagcacgtct gcgaaatacc tgacatagat
     4021 catataagtt gcgactccgc attttgggtg acagagatgg acaacggtct cgaatctggc
     4081 catggcgcac acggccagcg ggactgcgca gcaactccat caaatcattc gcatgaatga
     4141 agatgtgaag accattctcg caaccgcgga agcaatcaac cttctcgcgc tcaacgcatc
     4201 tgtaactgcg cgaagaattg gatcacttgg ccgagggttt gtgcaggttg catctgagct
     4261 aaccgaattc agccgcgaaa ccagacgcac tatgaacaat ctgc
//
LOCUS       DAORFF010000332         4297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12111_length_4297_cov_3.168317, whole genome shotgun sequence.
ACCESSION   DAORFF010000332 DAORFF010000000
VERSION     DAORFF010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12111_length_4297_cov_3.168317"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PKK76_12900"
     CDS             <1..937
                     /locus_tag="PKK76_12900"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HNL69422.1"
                     /translation="GHTGEGVSGTEVKSLFAFIFCLGAFPLHAIVPVPPLQGRVQDLT
                     ETLTAEEKTILEQKLADFEKTKGSQVVVLIVPSTEEEAIEQFSIRVAEAWKIGRKGVD
                     DGVILLVAKSDKKLRIEVGYGLEGALPDAICKRIIDEQIVPRFRAGKFGEGITAGVDS
                     ILQRINGEPLPAPFVSNSQDLTGIPWTMQLIVTAFLSIFIIVGSLVPSAVNKFLCILP
                     VAAGLAAAVAGYVVGGHWIVAVMFAAFATFFVGVFTTVMSGFFLSIFGLITGKKVGGW
                     RISTSSGSSGWSSSSGSSFSGGGGSFGGGGASGSW"
     gene            987..1442
                     /locus_tag="PKK76_12905"
     CDS             987..1442
                     /locus_tag="PKK76_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69423.1"
                     /translation="MATSTEKKKQTDRDLWRELTTRKEEFSKIVKLLVEFDEKTGRYL
                     KYNHPETHYMRRAVAACDPENLRIFLKSLGGFVYSVVAEVKSAAGLTATAWVHEDGIR
                     EEREGCDTGHPVHQIVCVTDLFEADSQTIDVVHSCVSQPAADLLAERGE"
     gene            1487..2230
                     /locus_tag="PKK76_12910"
     CDS             1487..2230
                     /locus_tag="PKK76_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNL69424.1"
                     /translation="MIEITGLKKSFKGMPVLTGLDLTIQDDETMAVIGPSGCGKSVLL
                     KHLVGLLRPDEGKIIIDGEDITRYSDSKLYDLREKFGMLFQSAALFDSLSVGENVGLW
                     LKEHTKTPETEIAVKADRILNLVGLRDVMEKRPSELSGGMKKRVGLARAIINEPKYVL
                     YDEPTTGLDPIMSDIINDLIIKVRMELKNTAIVVTHDMASVYKVADRIAMMHQGKIVF
                     LGKPDDIKTTDNPLAQQFIQGKAQGPIRL"
     gene            2277..3068
                     /locus_tag="PKK76_12915"
     CDS             2277..3068
                     /locus_tag="PKK76_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNL69425.1"
                     /translation="MHQTMIELKAVKKRFGAREILRGVDIDIRRGETMVLLGGSGTGK
                     SVTIRHIIGLLEPDEGEVRVDGHLMSGATKNTKTKLRARMGVLFQSGALINWLTVYEN
                     VALPLRETSKKTPKEIDEIVREKIAWLGLTPAMNLLPDKISGGMKKRVGVARALTTQP
                     EIILYDEPTSGLDPVMSQVLNELNNRLRDELKVTQVVVTHDMNSAFTIADRITFLYQG
                     KVRCVGTPRDIQESTDPVVQQFIKGETVGPMQIDAGDPTAKKADS"
     gene            complement(3065..>4297)
                     /locus_tag="PKK76_12920"
     CDS             complement(3065..>4297)
                     /locus_tag="PKK76_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNL69426.1"
                     /translation="EELAAAAENVGERAERLALVSKRIEENVRNLGKSIHEISRNMDQ
                     ARSISNEALTRAAAGRRSMDEGLVTMEHVQERAERIHEVVGVINGISERTSLLALNAS
                     IEAARAGDLGRGFAVVASEISKLADHAATSAEEIARLIEETSTEMKSSSESVRGVLGI
                     LTGLEDGTRQMAAAFEQIGESLKSQDRSAVEITKEASEIVDSSGDIDQAMREQRKTTQ
                     EISAGVETSSNSSRILAEDADRLNRIVGQIRNLMLMAERVLSRFKMPEVGRVLQWSES
                     YSVGVPQMDAEHAKIFEIMNQLTWMVKEGGSDAEQMRHLMDDLSEYCKFHLSSEEQFM
                     HDHRYPALDEHMQLHADFVKTVEGYGARIHDGEDLRFLAFEMCGTIWLWLTNHILVED
                     RKYGTFINSRRALQSGPR"
BASE COUNT         1065 a         1031 c         1121 g         1080 t
ORIGIN      
        1 tggacatact ggagaaggtg tttcaggaac tgaagtgaag tcgctgtttg cgtttatctt
       61 ttgtctgggt gcctttccgc tccatgcaat tgtgcctgta cctcctctgc agggcagagt
      121 tcaggatctt acagaaacgc tcactgcaga agagaagacc attctcgaac agaaactcgc
      181 tgacttcgaa aaaacaaaag gctcgcaggt tgtagttctg atcgtacctt caacggaaga
      241 ggaggcaatc gaacaatttt cgattcgcgt cgcggaagcc tggaagatcg gaagaaaggg
      301 tgtagacgat ggcgtgattc tcctcgtcgc aaagtcggac aaaaagttgc gaattgaagt
      361 tggctacgga ctggaagggg cactgcccga cgcaatctgc aagcgaatca ttgatgaaca
      421 gatcgtgccg cgatttcgcg caggcaaatt tggagagggc atcactgccg gagtggattc
      481 catcttacaa cgcatcaacg gcgaacctct gccggctcct ttcgtttcaa attcgcagga
      541 cctgactgga atcccgtgga ccatgcaact cattgtaact gcattcttaa gtatttttat
      601 cattgttgga tctcttgttc cctcagccgt gaataagttt ctgtgcattc tccctgttgc
      661 agcagggctc gccgccgcag tggctggcta tgtagtggga ggtcattgga tcgtggctgt
      721 aatgttcgct gcttttgcca cgttctttgt tggtgttttt actacggtga tgagtggatt
      781 ctttctctcc atctttggat tgatcacagg caagaaagta ggcggatgga ggatcagcac
      841 ctcaagtgga tcgagcggat ggagctcgtc gagtggatcg agttttagcg gaggcggcgg
      901 cagctttggt ggcggaggag cttctggaag ctggtaggcg gaacgattga tgggtcctgt
      961 accttctgat gttgtcagga ggaaccatgg caacatcgac agaaaagaaa aaacaaacgg
     1021 atcgggatct gtggcgagag ctcacaacaa gaaaagaaga atttagcaag atcgtgaagc
     1081 tgctcgttga gttcgacgaa aagaccggtc gctatttgaa gtacaatcat ccggaaactc
     1141 actatatgcg gcgcgctgtg gcagcatgcg atccggagaa cctgcgcatt tttctaaaat
     1201 ctctgggtgg cttcgtatat tccgttgtgg ctgaggtgaa aagtgccgct ggtttgactg
     1261 cgactgcctg ggttcatgaa gatggtattc gcgaggaaag ggaaggttgt gacacaggcc
     1321 atccagtgca ccagatcgtt tgcgtcacag acctatttga agcggattct cagacgatcg
     1381 acgtggttca tagctgcgtt tcgcaacctg ccgcggacct gctggcagag agaggcgaat
     1441 aaaaagtacg tgcttccaaa caatcgcgcc cacaccctgg gcgcagatga ttgagatcac
     1501 tggactcaag aaatcattca aaggaatgcc ggttctcact ggcctggatc tgaccatcca
     1561 ggacgatgaa accatggctg tcattggacc gagcggatgc ggcaagagtg ttttattgaa
     1621 gcatctcgtt ggcctgttgc gcccggacga aggtaaaatc atcatagatg gagaagacat
     1681 cactcgctat tcagatagca agctttacga tttgcgcgag aagttcggca tgctttttca
     1741 aagcgcggcg ttatttgatt ctctttccgt aggcgaaaac gtcgggctct ggctcaaaga
     1801 acacacgaag acccccgaaa cggaaattgc tgtgaaagcg gatcgcattc tcaacctggt
     1861 tggcctgcgc gatgtgatgg aaaaacgtcc ttccgaactg agcggtggga tgaagaagag
     1921 ggtaggtctg gcacgcgcga tcatcaacga acctaaatac gttttatacg acgaacccac
     1981 aaccggactc gatccaatca tgtccgatat catcaacgac ctgatcatca aagtacgcat
     2041 ggaattgaag aatactgcca ttgtagtcac tcacgatatg gccagcgtgt acaaagtcgc
     2101 ggatcgcatt gcgatgatgc atcagggtaa aattgttttt cttggaaagc cagacgatat
     2161 caagacaaca gacaatccgc tggcgcaaca attcatacag ggcaaagcac agggaccgat
     2221 tcggctatga gttgcctggc caaacaaatt cgcctatatt gaacaaagga aagtccatgc
     2281 accagacgat gatcgaactc aaggcagtga agaagcgttt cggagctcgc gaaattttgc
     2341 gcggagtgga catagacatt cgtcgtggtg agacgatggt tttgctcggc ggttctggaa
     2401 cagggaagtc tgtaacgatt cgccatatca ttggtctact ggagccggat gaaggtgaag
     2461 tgcgcgtaga tggccatttg atgagcgggg caacgaaaaa tacaaagaca aagcttcgcg
     2521 ccagaatggg agtgctcttt cagagtggag ccttgatcaa ctggcttact gtgtacgaaa
     2581 acgtggctct tcctctgcgc gaaacgtcta aaaagactcc aaaagaaatt gatgaaatag
     2641 tgcgcgaaaa gatcgcatgg ctgggtttga cgcctgctat gaatttgctt ccggacaaga
     2701 tttcaggagg aatgaagaag cgcgttggag tagcgcgcgc tttgactact caacctgaaa
     2761 tcattctgta cgatgaacca acttctggcc tggatccggt catgagtcaa gtgctcaatg
     2821 aattgaacaa tcgtttgcgt gatgaactga aagttacgca ggtggttgtg acccatgata
     2881 tgaattccgc tttcacaatt gctgatcgca taacgttcct ctaccaggga aaagtgcgat
     2941 gcgtcgggac ccctcgggat atacaagaat caacggatcc tgtcgtgcaa cagttcatca
     3001 agggagaaac tgtgggtccc atgcagatcg acgcgggtga tcccacagca aaaaaggcgg
     3061 atagttagcg cggtccggac tgcagagctc ttcggctatt gataaacgtt ccgtactttc
     3121 tgtcctccac cagaatatga ttggtaagcc agagccatat ggttccgcac atttcaaacg
     3181 cgagaaaacg taaatcctcg ccatcatgga tgcgagcccc atatccttcc actgtcttta
     3241 cgaagtctgc atgaagctgc atgtgttcgt ccagtgccgg ataacggtga tcatgcatga
     3301 actgttcttc gctggaaagg tggaatttac agtattcgga aagatcatcc atcaagtgtc
     3361 tcatctgctc tgcatctgag ccgccttcct tcaccatcca agtcaactga ttcatgattt
     3421 caaaaatttt cgcatgttcc gcatccattt gggggacccc gactgaatag gattcagacc
     3481 actggagcac tcttcccact tcaggcattt tgaaacgaga gagcactctt tctgccatca
     3541 acattagatt tcggatttgt cctacaatgc ggttcaaacg atccgcatct tctgccagaa
     3601 tccgggaaga gttggatgat gtttccacgc ctgccgaaat ctcttgcgtg gtttttcttt
     3661 gctctcgcat tgcctggtca atgtccccgg aagaatccac aatttcagaa gcttctttcg
     3721 tgatctccac cgcgctcctg tcttgagatt tgagtgactc tccaatttgt tcaaatgcgg
     3781 ctgccatctg ccgggttcca tcttcgagtc ctgtaagaat tcccagcacg ccgcgcacac
     3841 tttcgctgct ggatttcatt tcagtagaag tctcctcaat gagtctcgcg atttcttccg
     3901 cactcgtggc cgcgtggtcg gcaagtttgg aaatttcgga ggcaactacg gcgaaccctc
     3961 gacccagatc ccccgcccgc gccgcctcga tggatgcatt caatgcaagc aggctcgtgc
     4021 gttctgaaat tccgttgatg actccaacaa cttcgtgaat tcgctctgct ctttcctgta
     4081 catgttccat ggtaacgagg ccttcgtcca tggatcttct gccggcggct gctcgcgtaa
     4141 gcgcttcatt ggaaatagaa cgagcttgat ccatattccg ggagatttcg tggatgctct
     4201 ttcccaggtt tcttacgttt tcttcaattc ttttgctaac gagtgcgagg cgctccgcgc
     4261 gctcgcctac attttctgcc gctgccgcca gctcttc
//
LOCUS       DAORFF010000333         4289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12142_length_4289_cov_3.688710, whole genome shotgun sequence.
ACCESSION   DAORFF010000333 DAORFF010000000
VERSION     DAORFF010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12142_length_4289_cov_3.688710"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PKK76_12925"
     CDS             <1..954
                     /locus_tag="PKK76_12925"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNL69427.1"
                     /translation="EGGDFRLDARAAVRQATGEDWNNVRITLSTARPSVALAPGQLRP
                     WRISGKDLWTMDRDEGKRDDSAGETESSAGDSQDGASFSIQLPARETIESDNSDHRIT
                     LQSTPLKGTVNHVAIPSMTDSVFLRAKFRNSSSIPLLTGAMNIFLDGSFAGTTTTPRA
                     AAGEDFDLYLGADQRMTARRVLLKGEVAGAGILSSKVQIQNQWQIEIANHSRKPRSVI
                     VFDRYPVARDPNISTKFQGSNRSDVKPDANGMLAVNMNVDPGASQKFDFSYSLEIPQE
                     TWQKFQENLENEKGRRPAQPAVEPNAPAKQRAYDLERMLMH"
     gene            1467..1802
                     /locus_tag="PKK76_12930"
     CDS             1467..1802
                     /locus_tag="PKK76_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69428.1"
                     /translation="MRKPILLSIMFLLILSCKGQPVPENKKSYVGTWVGDGITLIISS
                     DGGLSYQKVRSAGRTSVHGPIQEFAEREIRAGVWIFSAKFRIDQEPALVDGKWMMEVD
                     GEKLTKLGP"
     gene            complement(1838..2869)
                     /gene="plsX"
                     /locus_tag="PKK76_12935"
     CDS             complement(1838..2869)
                     /gene="plsX"
                     /locus_tag="PKK76_12935"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNL69429.1"
                     /translation="MAFVLHGRMTAWVAVDTMSGDHGPEPAVHGAQQAIREYGSHVTL
                     VGDPEKLEKLLKRFTCDPSHVQIAPAKEVITMDDSPARAVRSKPDASVVVAARLVKEK
                     KAVGFFSPGNTGATMAAALLEMGRIKGVERPSIASPLPREDGGTTVLIDSGANVDCKA
                     SWLVQFAIMGEIYSREILGVVNPRVAVLSNGEEEGKGNSVSTDAWERLRHLPCNFMGN
                     VEGRDLYGGGRTADVVVCDGFTGNIVLKATEGLATSIFTILLQGIAGSSLAKTGAYLL
                     KPVLSEVKRRMDYTEYGGAPLLGVEGICIIGHGGSNATAFKNAIRVVDQSSKRDIRQK
                     IEDRVRLFG"
     gene            2927..3667
                     /locus_tag="PKK76_12940"
     CDS             2927..3667
                     /locus_tag="PKK76_12940"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNL69430.1"
                     /translation="MRAIVFFLCLTTCTTAGVMLPVNDVACKTGGRRVPDGAIPTPED
                     LAKDAFCAQRVIDRAAPHGVVTIFGSARAKEDWDSYKITRKFAYLWSKENSRYPILTG
                     GGPGLMEAGNRGAKEAGAPSLYFSTYFGSGSEKPNSYTTDGFMFASFAQREADMVDRA
                     AAVVIGQGGVGTEWEIFETLSKVQTRKKARCPIVLLGKRSDWASLFARMDAMKQSRTV
                     SPGDSDLMVVAETPEEAVAIIRAGIRDL"
     gene            3664..>4289
                     /locus_tag="PKK76_12945"
     CDS             3664..>4289
                     /locus_tag="PKK76_12945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL69431.1"
                     /translation="MIEALKAFIRPAPEMPRIQDPTRIKSDYRYWRTRIMYSMMSGYA
                     VYYFVRKNFSIANKSISEEFHFSNTEMGLILSAATVAYGISKFASGILVDILNPRFLM
                     SAGLILSALTNIFFGMGAGLTVFIVLWALNNLFQGLGMPPCGKLLNSWFSRKEGGRAW
                     GLWNASHQIGGGLIFLIAGFLVSEYGWRSSFYVPGVFAILVGLFLINRL"
BASE COUNT          993 a         1097 c         1134 g         1065 t
ORIGIN      
        1 gaaggaggcg atttcaggtt ggacgcccgg gccgctgttc gccaggccac aggagaagat
       61 tggaataatg tgcgcataac gttatccacc gcaagaccgt cagtcgctct tgctccggga
      121 cagcttcgtc catggagaat ttctggaaaa gatctgtgga ccatggatcg cgatgaagga
      181 aagcgcgacg attcagccgg agaaacagag tcgagtgccg gtgattccca ggatggagct
      241 tctttttcta tccaactccc tgctcgcgaa accattgaat cggacaactc agatcatcga
      301 atcaccctcc agagcactcc tctgaaaggc actgtcaatc atgtggcgat tccctctatg
      361 actgattctg tatttcttcg tgcgaagttt cgtaattctt cttccattcc tcttttgact
      421 ggtgccatga acatctttct ggacggaagt tttgccggaa cgactactac acctcgtgcc
      481 gccgcaggcg aggacttcga tttgtatctg ggtgcagacc agcgcatgac cgcgcgtcgc
      541 gttttgctca agggagaagt ggctggcgcg ggcatactat cgagcaaagt gcagatccaa
      601 aaccagtggc aaatcgaaat cgcaaatcat agtcgaaagc ccaggtctgt catcgtgttt
      661 gatcgctatc ctgtggcgcg agaccccaat atctccacga agtttcaggg gtcgaaccgg
      721 agcgacgtga agccggatgc aaacggcatg ctggctgtga atatgaacgt ggatccgggc
      781 gcctctcaaa aatttgactt ctcttattcg ctggagattc cccaggaaac gtggcagaag
      841 tttcaggaaa atctggagaa cgaaaaagga aggcgccccg cccagccagc ggtagagccg
      901 aatgctcccg ccaaacaacg cgcatacgat ctcgagagaa tgctgatgca ttgaagctct
      961 ctggcgtgcg tgtgctttat cacgcaacaa aagtgcgtga taacaccggg cgttggtaag
     1021 tataacgaac attttatgcg tgataaaccg ggcgcggcgc ggccgttccc ggtcctctcg
     1081 cgtgcgtgtg ctttatcacg caataaaagt gcgtgataac accgggcgtt ggtaagtata
     1141 acgaacattt tatgcgtgat aaaccgggcg cggcgcggcc gttcccggtc ctctggcgtg
     1201 cgtgtgcttt atcacgcaat aggagtgcgt gataacaccg ggcgttggta tgtaaaacga
     1261 acattttttg cgtgataaac cggcgcggcg cggccgttcc cggtcctctc gcgtgcgtgt
     1321 gttgtatcac gcaacaaaag tgcgtgataa caccgggcgt tggtaagtat aacgaacatt
     1381 ttatgcgtga taacgcgatg cggatcggaa gttcgccttt gcagacaatg cttgcaaaca
     1441 tacgcgccta tgtctcaata tcagtcatga ggaaaccaat tctactttcg attatgtttt
     1501 tgctgattct ttcgtgcaaa ggacagccag ttccggaaaa caagaagagt tacgtaggca
     1561 cgtgggtggg tgacgggatt accctgatca tcagcagtga cggggggctc agctatcaga
     1621 aggtgcggag cgccgggcgc acatcggtcc atggtcccat ccaagagttt gcagagcggg
     1681 agattcgtgc cggtgtctgg atcttttctg cgaagttccg aatcgatcag gagccagcac
     1741 ttgtagacgg gaaatggatg atggaagtgg acggagagaa actgacgaag cttgggccct
     1801 gaatcttgcc tggcaggccc ccgtttcctt acgggcttca cccgaaaaga cgcactctgt
     1861 cttctatctt ctggcggatg tctcttttgg aggattgatc caccacccga attgcgtttt
     1921 tgaatgcagt tgcgttggaa ccaccgtgac ctatgatgca gattccttct accccgagca
     1981 aaggcgctcc cccgtactcg gtgtaatcca ttcttcgttt cacttctgaa aggacaggtt
     2041 tgagcaaata cgctcccgtc ttcgcaagac ttgatccagc gatgccctgc aatagaatcg
     2101 taaatatcga tgtggccaga ccttcggttg ctttgagcac aatgttgcct gtgaatccgt
     2161 cgcacacgac gacatccgca gttcgtccgc ctccgtatag atctcttcct tccacattgc
     2221 ccataaagtt gcacggcaaa tgtctcagtc tctcccatgc atctgttgaa acagaatttc
     2281 cttttccttc ttcttctcca ttggagagaa cggcgacgcg cgggttgacg actcccagaa
     2341 tttcgcgtga atagatttct cccatgatcg caaattgaac cagccagctt gccttgcagt
     2401 ctacgtttgc gcctgaatca atgagcactg tggttccccc gtcttcgcgc ggcagagggc
     2461 tcgcgatcga tggtctctcc actcctttga ttcgccccat ttcaaggaga gccgccgcca
     2521 ttgtcgcacc agtatttccc gggctgaaaa aacccactgc cttcttttcc ttcacgaggc
     2581 gagccgccac tacgacagat gcgtctggtt tggatctcac cgctcgagcc gggctatcgt
     2641 ccattgtgat tacttctttg gcgggcgcga tttgaacgtg cgatggatcg cacgtgaagc
     2701 gtttgagcag tttctctaat ttttcaggat cacctacgag agttacgtga gagccgtatt
     2761 cgcggatggc ctgttgcgcg ccatgaaccg caggctccgg accgtgatct ccgctcattg
     2821 tgtctacagc tacccaggca gtcatgcgcc cgtgtaacac aaaagccatg aatcgtaaag
     2881 aatttaactg ttgcaaaaca aatgatgagg accaaacaag tgccccgtga gagccatcgt
     2941 attcttcctc tgtttaacga cctgcaccac agccggcgtc atgcttccgg tcaacgacgt
     3001 tgcatgcaag acgggcggta gacgcgttcc ggacggagcc atacccactc cagaagacct
     3061 ggccaaagac gccttttgcg cgcagagggt gatcgatcgc gcggcccctc acggagtcgt
     3121 taccatattc ggatctgccc gcgcgaaaga ggactgggac tcttacaaga tcacgagaaa
     3181 gtttgcttat ctgtggtcca aagaaaatag ccgttatccg atcttgacag gaggcggacc
     3241 cggtctcatg gaagcaggaa atcgcggcgc gaaagaggcg ggagcgccca gtttgtactt
     3301 ctcaacttat tttggatcgg gctctgagaa gccaaactcg tacactacag atggttttat
     3361 gtttgcaagc ttcgctcagc gtgaggcgga catggtagac agagcggcag cggtggtcat
     3421 cgggcaggga ggcgtgggca cagagtggga gatttttgag acactctcca aggtgcaaac
     3481 tcgcaagaaa gctcgttgtc ccatcgttct gctcggaaaa agatccgatt gggccagtct
     3541 tttcgctcgt atggatgcca tgaagcagtc tcgcacagtc tctccaggtg attcagatct
     3601 catggtggtg gcagaaacac cagaagaagc agtggccatc attcgtgcgg ggattcggga
     3661 tctatgatag aagctctaaa ggcattcatc cgtcctgctc ctgaaatgcc tcgcattcag
     3721 gaccccacac gaatcaagag cgactatcgt tattggcgaa cacgaatcat gtatagcatg
     3781 atgtccggct atgctgttta ctattttgta agaaagaatt tctccattgc aaataaatcc
     3841 atttccgaag agtttcactt ctcaaataca gagatgggac tcatcctcag tgcagccaca
     3901 gtcgcctatg gaatttcaaa attcgcaagt ggcattctgg tagatatcct caatccacga
     3961 tttctcatgt ctgcaggctt gatcctttcc gctttgacga atattttctt tggaatggga
     4021 gccggactga ctgtattcat tgtactgtgg gcactcaaca atctatttca agggctgggc
     4081 atgcctccct gtggaaagct tttgaattcc tggtttagca ggaaggaagg cgggcgagcg
     4141 tggggtctat ggaatgcgtc gcatcagatc ggcggtggtc tgatctttct cattgctgga
     4201 tttctcgtca gcgaatatgg ttggagatcc tctttctatg ttccaggagt ctttgctatt
     4261 ctggttggac tctttttgat caatcgcct
//
LOCUS       DAORFF010000334         4284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12155_length_4284_cov_3.779144, whole genome shotgun sequence.
ACCESSION   DAORFF010000334 DAORFF010000000
VERSION     DAORFF010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12155_length_4284_cov_3.779144"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3236
                     /locus_tag="PKK76_12950"
     CDS             <1..3236
                     /locus_tag="PKK76_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69432.1"
                     /translation="AIDELGESETPAVELPLAMEVGMEDSGGWKEDSASEIADVQTDD
                     FAEAFSETSALKEDESQHNADIPGEVDVRDDSVLDFASTSAEPFHSDSILATGAEALE
                     SPSTDYAADTDSTFSNLEVSEETLASEDSLRDHLARPDHRTDLDLKSDAAYKQFLIEQ
                     SELTQLRKERFLELSGLPGRIDQAIEQRNESESVQLLAEIHPLAFLTEQERLLRGLDA
                     IESPLGKIMGHLSEMYRRLNTPDMREYVDYYSAKLEAGGLFDTFPTIRERLINYRNSL
                     VDPPPELLKQVHTGLRDIEDRTGEDHRECVAQQVRFLHECLENEVFGKVWPTIAVILE
                     NILKEELASLGETDAPIVSWRNRLQREEQTTPRAIANILAEVEPESGSAIAGILSTTD
                     LPSATARPPASGLSSATPGHRGTGFDFLANRSLDPHGDAQKMQESLTRQFATDAHAAH
                     ALSIQILNSSEYSLLEKETTLSVLERANLAAATELYHFDRLVSSAGTLDDQLAFAASL
                     RNGSAGHLVADRLQSLEFRRAQNQSMGHFSAFLATKDPSAKTRLFQEIARDPHIPEGM
                     KSVFRKGADANSFEKFLESITTSSQSNANKLKLLRILQKIAVHRNWLTPSTKERLENR
                     IRFYERILSADQEIAASVVGRLDYYRTRREEIRQALMTGKPAREKLTPPNVESPAVES
                     AEEVHRHGFSDELLESLIQSPMRASRWYSKLPESSKEELHKQATSPGKAQILVNQIQK
                     DIAFKKAIRHLKKPVEAAVFSELSAQFPEPEYQEKLRQFTPSQKETKVVVAQSQSASE
                     DSSTNIPSGAIGPDASFDQLVNQTLLSGPDLPPLPEEDLALDHRPGPPHPAFAAAISK
                     NEERTKHDVTSSKPTPPHPQHHAEPSHHEGTRHEPHHERHPEKEHPSHAARAKTESAV
                     DGLAASLARGFSGLFSKKTSAPSHGKEQKAQSSSHGAHDKKSGKKKTDQAAATLMKAV
                     SSYAKGEIYLSEKDLKSLEKEMEKHPNAKRLIEDHAYLFKVEHPDPAQFQPPIFVIIP
                     EMKALNHDPTKKKAVMTRIEQSISSAPSKKKAHMQALLTFMERYKTK"
     gene            complement(3286..>4284)
                     /locus_tag="PKK76_12955"
     CDS             complement(3286..>4284)
                     /locus_tag="PKK76_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69433.1"
                     /translation="FVRFARTWSWLENKLLPDAYRLVMSARAFGGDNFARIFYDILNF
                     LPPLRSPAYPEKKLTLDDIYRDSKMIRFRLRLKKKKRVPPPPVRKKFQREKYPGEWRD
                     SFKGGGICSYPPEDIVIEDFGRYLQKRAVSVLQGSDSKTLPFTASLLDGIDYRETIRN
                     LHMGKVFVRDLRNRGIEAGSVVLIFSEDDIEHDWKVVWWGEHNQESDMALYATSPGNE
                     IIGPGICRCRYGGLMMTYPPRRLHDIWEDPNYNEFEKPSDRLLAAAIDYNEKNAVVHL
                     AHRPPSPKLVAIANRLGQKVIHIPISTMNSVLLGRIRRFHVLDSHDRRGDAGGFIW"
BASE COUNT         1204 a         1128 c         1040 g          912 t
ORIGIN      
        1 aagcaatcga tgaattgggc gaaagtgaga caccagcggt cgagcttccc ctggcgatgg
       61 aagtgggcat ggaggattcc ggcggatgga aagaagattc ggcgtcagaa atcgcagatg
      121 tccagacaga cgatttcgca gaggcgtttt ccgaaacaag cgcactcaaa gaagacgaga
      181 gccaacacaa tgcagatatt ccaggcgaag tcgatgtgcg ggatgactct gtgctggatt
      241 ttgcatccac ttcggctgag ccttttcatt ccgattcaat tctcgcaaca ggcgcagagg
      301 cactggaaag cccgagcaca gattacgcgg cagacacaga ttctactttc tccaatctgg
      361 aagtgtcaga agaaacactc gcctccgaag attcactccg cgatcatctc gctcgccccg
      421 atcatcgcac cgatctcgat ctgaaatcgg acgctgcata caaacagttt ctcatcgagc
      481 aatccgagct cacgcagctt cgcaaagaaa ggtttctgga actgtccgga cttccggggc
      541 gaatcgatca agccatcgag cagcgcaatg aatcggaatc cgttcaattg ctcgcggaga
      601 ttcatccgct tgcatttctc acggaacaag aacggttgct tcgcggtctc gatgcgattg
      661 aaagcccact tggaaaaatc atgggacatc tttcagagat gtatcgtaga ctcaatactc
      721 cggacatgcg ggagtatgtg gattactatt cagcaaagct tgaggccggg ggactcttcg
      781 acacgtttcc aaccatccgc gaaagattga tcaactatag aaacagcctg gtagatcctc
      841 cgccagaact actcaagcaa gtccacactg gtctgcgcga catcgaggac agaaccggcg
      901 aagatcatcg cgaatgcgtg gctcagcaag tgcgatttct acatgaatgt ctggagaacg
      961 aagtcttcgg aaaagtctgg cccaccattg cagtgatcct ggaaaatatt ctaaaagaag
     1021 agctcgcctc gctcggagag acagacgctc ctatcgtttc gtggcgaaac agacttcaaa
     1081 gagaagaaca aaccactccc agggccatcg caaatattct cgcggaagtg gaaccggaga
     1141 gtggatctgc cattgcaggt atcctttcta cgacggacct tccttctgca acggcccgtc
     1201 ccccggcgag cggcctttca tctgcaacgc cgggtcatcg aggcacaggt tttgattttc
     1261 ttgcaaatcg ctctcttgac ccgcatggag atgcacagaa aatgcaggag tcactgacca
     1321 gacaattcgc gacagacgcg cacgctgctc atgctctttc gattcagatt ctcaattcga
     1381 gcgaatacag ccttcttgaa aaagaaacca ccctttccgt actcgaaagg gcgaatctag
     1441 ctgcagccac ggagctatat cattttgaca gactcgtatc ttctgccggg acgttagacg
     1501 accaactggc attcgctgca tctttgagaa acggcagcgc cggtcacctt gttgcggata
     1561 gacttcagtc ccttgaattt cgtcgcgctc aaaatcaatc gatgggccat ttttctgctt
     1621 ttcttgcaac caaagacccg tcagccaaga cccgtctctt tcaggaaatt gcgcgtgacc
     1681 cgcacattcc ggaagggatg aagtctgtct ttcgcaaggg agcagacgca aactctttcg
     1741 agaagttcct ggagagtatc accacctcaa gccaatccaa cgcaaacaaa ctgaaactac
     1801 tgagaattct gcaaaaaatc gcagtccata ggaactggct gactccatca acaaaagaaa
     1861 gactggaaaa caggattcga ttttacgaac gaatcttaag cgcggatcaa gagatcgccg
     1921 ccagtgtagt tggtcggctg gactactaca gaacgcgcag agaggaaatc agacaagccc
     1981 tcatgaccgg gaagcctgca agagagaaac taacgccgcc taacgtagaa tctcctgcag
     2041 tagaatctgc agaagaagtg caccggcatg gattcagtga cgaattgctg gagagtctaa
     2101 tccagagtcc aatgcgcgca tccaggtggt attcgaaact gccggaaagc tcaaaagagg
     2161 agttacacaa acaggccact tccccgggta aagctcagat ccttgtcaac cagatccaga
     2221 aggacatagc attcaaaaag gcaatacgtc atttgaaaaa accggtagaa gccgccgtgt
     2281 tttctgagct ttccgcccag tttccggagc cagaatacca ggagaagctg aggcaattta
     2341 cgccgtctca aaaggaaacg aaagtagtgg tcgcgcagtc gcaatcggca agcgaagatt
     2401 catctacaaa tataccgtca ggcgccattg gtccagatgc tagttttgat caactggtaa
     2461 accagaccct gctatccggt ccagatcttc cacctcttcc ggaagaggac ctggccctgg
     2521 accatcgccc gggaccaccg catccggcct tcgcggcggc catttcgaag aacgaggaac
     2581 gcacaaagca cgacgttaca tcttcaaagc ccactccacc tcatccacag catcatgcag
     2641 agccttcgca tcatgaaggc acgcgtcacg aaccacatca cgaacgtcat ccagaaaagg
     2701 agcatccttc acatgcggct cgcgcgaaga cagaaagtgc agtggacggc ctggctgcgt
     2761 ctctcgcgcg cggtttcagc ggcctgttct caaagaaaac aagcgcgccc tcgcatggaa
     2821 aagagcagaa ggcgcagtct tcttcccatg gcgcgcatga caagaaatca gggaagaaga
     2881 aaacagatca agcggccgct actctcatga aggcagtttc atcgtacgcg aagggagaga
     2941 tatacttgag cgaaaaggat ttgaaatctc ttgagaagga aatggaaaaa catccgaatg
     3001 caaaaaggct gatcgaagat cacgcttact tatttaaggt ggagcaccca gacccggctc
     3061 aatttcagcc tcctatattc gtcatcattc cggaaatgaa ggcgttgaac catgatccta
     3121 ctaagaagaa agctgtgatg accaggattg aacagtcgat cagcagcgcg ccttccaaaa
     3181 agaaggctca catgcaggcg ttactcacgt ttatggaaag atataagacg aagtgaagtt
     3241 gggagaacca gtggcgagcg ctgccagaaa ggccaggcgt tcaccttacc agatgaagcc
     3301 gcccgcatcc cctcttctat cgtgcgaatc aagtacatga aatctgcgaa tgcgtccaag
     3361 cagcacgcta ttcattgtag aaatcggaat atgaatcact ttctgaccca gacgatttgc
     3421 aatggcgact aacttcggag aaggtggccg atgcgctaga tgaaccactg cattcttctc
     3481 attgtagtca atggccgccg caagaagacg atccgatggt ttttcgaatt cattgtaatt
     3541 gggatcttcc cagatgtcat ggagtctcct tggtggatag gtcatcatga gcccgccgta
     3601 gcgacagcgg caaatgccag gcccgatgat ctcatttcca ggagaagtag catagagcgc
     3661 catgtcgctt tcctgattgt gttctcccca ccagaccacc ttccaatcgt gctcgatgtc
     3721 atcttcgctg aaaatcaaaa ccacactgcc ggcctcaatt cctcgattgc gcagatcgcg
     3781 cacaaatact ttacccatat gcaagttgcg gattgtttcg cgataatcaa ttccatcgag
     3841 caaactggcg gtaaacggca gagtctttga atcagaaccc tgcaagacag agacagcgcg
     3901 tttctgtaaa tagcggccaa agtcctcaat cacaatgtct tccggcgggt aagagcaaat
     3961 tccgccaccc ttgaaagaat ctcgccattc acccggatac ttctcgcgct gaaatttctt
     4021 gcgaactgga ggtggaggga ctcgcttctt cttcttgagt cgaagtcgaa atcgaatcat
     4081 tttactatcg cgatagatgt cgtctaacgt taattttttc tcaggataag ccggactgcg
     4141 aagaggagga agaaagttca gaatgtcata aaagatacga gcaaagttgt ctccgccaaa
     4201 agctctcgca gacatcacca gcctgtaagc atctggcaga agcttatttt ccagccagct
     4261 ccaggttcgc gcaaaacgca caaa
//
LOCUS       DAORFF010000335         4262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12259_length_4262_cov_4.113620, whole genome shotgun sequence.
ACCESSION   DAORFF010000335 DAORFF010000000
VERSION     DAORFF010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12259_length_4262_cov_4.113620"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PKK76_12960"
     CDS             <1..710
                     /locus_tag="PKK76_12960"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNL69434.1"
                     /translation="AEGEDMVYRAVSGNAKPFLGLRIKRNGSLSGLCVQTGEVLLCED
                     SETDPRVDRQTTRKIGLRSMIVMPLRYRDATVGVLKVMSAEAHKFQKEDEMILGLLSE
                     VVAAAMFHATRLESDDLLHKATHDALTDLANRSLFMDRLRNILSSSSNQAGLLMIDMD
                     GLKTINDSLGHRADELSCLLSENSLLKDRPFPFMQASAGQAFQRILWIWRNSLKPLTV
                     ECMRPSEVIDRTPHPIP"
     gene            complement(756..2369)
                     /locus_tag="PKK76_12965"
     CDS             complement(756..2369)
                     /locus_tag="PKK76_12965"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNL69435.1"
                     /translation="MSFVGTKYKQEEVDSHYDAVIIGSGMGSLSTAALLSKAGKRVLV
                     LEKHYTAGGFTHSYTRKGYEWDVGVHYIGEVHKRLSPLRLLFDLVTDGKLEWSYMGDT
                     YDSVFFGEERYDFMAGEEQFMANLLRHFPGEKDTLKNYIKLIKSANRLAIPFYVSQKI
                     FPDAVGKMASSLSDSILAKYVRRTTGEVLADLTGNKKLRGVLTAQWGNYGLPPAQSSF
                     MIHAAVAHHYLDGGNFPAGGASQIARTIEKVIQKPGGKILVNAPVSQILVNGSRAMGV
                     RLENGHEILADRVISGAGYFNTFGKLLSTETAEKVGGGSKLKTVRPSIGHVCLYTGMK
                     HDAEELKLPMGNLWVYQDYDHDASLARFLEKPNLDFPLVYISFPSAKDPLWKTNHPGR
                     STIDVIAPMPYAWFEKWKGTEWNKRGDEYENYKESISKKLLEKIYKYVPQAEGKISYY
                     ELSTPLSTEHFGNYSRGELYGIDHNVGRFEQNWMKPQTQIENLYVTGQDILFCGVASA
                     LSSGVMTAASILGSEIGSIVPGFANHFRS"
     gene            2488..3075
                     /locus_tag="PKK76_12970"
     CDS             2488..3075
                     /locus_tag="PKK76_12970"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNL69436.1"
                     /translation="MKKPYHHGNLKEELIAAGISVLKKEGTGGLRLREVAGLVGVSHA
                     SLYRHFTNKEELLAAIAEQGFRGLIENVELYKKRAGSNFKSQFIESGRAYVDFAASHK
                     EQFHIMFSGIIPDRKKHPALALAAEESFGQLVQIVEECREAGVIPSHRDRKVHTLAAW
                     SMLHGIATLLIEGQIPGGRSRTRAITRSLLILLTV"
     gene            3173..3649
                     /locus_tag="PKK76_12975"
     CDS             3173..3649
                     /locus_tag="PKK76_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69437.1"
                     /translation="MKIRSFLNIAALSLAFAFSTASLRAEPVVLTEDVLKRYMAIFPS
                     YMRITLELDGTSKMREGAAKEAKVTELIEKRNALLTANGWEDFSEYMEVDARVVRAMT
                     PLGILEKLKNASEPDRKKAEDTVAAQLQDYTPEEIALVKKNLVGLTKMRRDALAGR"
     gene            3738..>4262
                     /locus_tag="PKK76_12980"
     CDS             3738..>4262
                     /locus_tag="PKK76_12980"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL69438.1"
                     /translation="MRPWIRACFISLLSSLAAQCNQSSKCKEADASCNVLPLLLYSKV
                     AVATNLYVVNATSGSLSEYSIDRGTGLLSSVGTATGMAGTNGVQVDPLGRFVFVAAST
                     TPRIHRFAIDTTGALSVLGTTTPNSTFPIGLCADPTGAYLFSVVAGNGTVEGFSVSSN
                     SVLTSTGTALAAAGG"
BASE COUNT         1037 a         1114 c         1060 g         1051 t
ORIGIN      
        1 tggcagaagg cgaagatatg gtctatcgcg ccgtatcggg aaacgcgaaa ccatttctcg
       61 gtttgcgaat caagcgcaac ggaagcctat cgggcctgtg cgtacaaacg ggtgaagtct
      121 tattgtgcga agactccgaa acagacccga gggtggatcg ccagaccact cgaaagattg
      181 ggttacgatc catgatcgtt atgccgctgc gataccgcga tgcaacagtc ggtgttttga
      241 aagtcatgtc cgcagaagct cacaagttcc agaaagaaga cgaaatgatt ctgggtttgc
      301 tgtctgaagt cgtggccgcg gccatgtttc atgcaactcg cctcgaaagc gacgatctct
      361 tacacaaagc cacacacgac gcattgacgg atctggccaa ccgttctctg ttcatggatc
      421 ggctgcgaaa tatcttgagc tcgagtagca atcaagcagg ccttctcatg atcgacatgg
      481 atgggctcaa gacgatcaac gattctcttg ggcaccgtgc cgacgaattg agttgtcttt
      541 tgagcgagaa ttcacttttg aaggacagac cattcccatt catgcaagca tcggcggggc
      601 aagctttcca gaggattcta tggatctgga gaaactcatt gaaaccgctg acagtagaat
      661 gtatgcggcc aagcgaagtc atagacagaa cgccgcatcc aatcccctga accaaccttc
      721 gaaacgaaac aatccagact aactccctgg acttctcagg accggaagtg atttgcaaat
      781 ccgggaacaa tcgatccgat ctcagaacca agaatgctcg cggcagtcat gactcctgaa
      841 ctcagagcgc tggctactcc gcaaaacaga atgtcctgtc ccgttacata tagattctca
      901 atctgagttt gaggtttcat ccagttttgt tcaaaccttc ccacattgtg atcaattccg
      961 taaagctcgc ctctggagta atttccaaaa tgctctgtgg acagaggtgt ggataattcg
     1021 tagtagctga tcttaccttc cgcctgcggg acatatttat aaattttttc aagcaatttc
     1081 tttgaaatgg attctttgta gttttcgtat tcatctcccc gcttgttcca ttccgttcct
     1141 ttccattttt caaaccaggc atacggcatg ggtgcaatca catcgatggt ggaacggccc
     1201 ggatgatttg ttttccagag cggatctttc gcactcggaa aagaaatata gacgaggggg
     1261 aaatccagat ttggcttttc caggaatcgg gcgaggctcg cgtcgtgatc gtagtcctgg
     1321 tagacccaga gattgcccat gggcaatttc aattcttcgg cgtcatgctt cattccggtg
     1381 tagaggcata cgtgaccgat cgacggacga accgtcttca atttggagcc accacctacc
     1441 ttctctgcgg tttccgtgga aaggagctta ccgaatgtat tgaaatatcc agcgccgctg
     1501 atcactcggt ctgccagaat ctcatgacca ttttccaggc gaacccccat cgcgcggctg
     1561 ccgttcacaa gaatctgaga tacaggtgca ttcaccagaa tcttcccgcc cggtttctgg
     1621 attacctttt cgatggtgcg agcgatctga gatgcgcctc ccgccggaaa atttcctcca
     1681 tccaggtagt ggtgagccac tgccgcatga atcatgaagc tagactgtgc tggaggcaaa
     1741 ccgtagttcc cccattgcgc agtgagaacc cctcggagtt ttttattgcc agtcaaatct
     1801 gcgagcactt cgccggtggt tcgtcgcacg tatttcgcaa gaatggaatc agatagactc
     1861 gatgccatct tgcccactgc gtccggaaaa atcttctggc tgacgtaaaa tggaatcgca
     1921 agtcgattgg cgctcttgat cagcttgatg taattcttga gtgtgtcttt ttccccggga
     1981 aaatgacgga gcaaattggc cataaactgt tcttcgcctg ccatgaagtc ataccgttct
     2041 tcaccgaaga aaacgctgtc gtacgtatct cccatataag accattccag cttaccgtcc
     2101 gttaccagat caaacagcag tctaagtgga gagagtcttt tgtgaacttc tccaatgtaa
     2161 tgaactccca cgtcccactc gtaacctttg cgcgtgtaac tgtgagtgaa tcctccggca
     2221 gtgtagtgct tttccagcac aagcactcgc ttccccgcct tggacaaaag cgccgccgtg
     2281 gacaaactgc ccatgcctga accaatgatc acagcatcgt agtgcgaatc cacttcttcc
     2341 tgcttgtatt tcgttccaac aaatgacata gggcctccct gtgctcgcca gcctgacatt
     2401 gcacaatgta accattggta acattgatgt ttcccgtggc aacattttta attgtaattt
     2461 tccctggcga ccgactgctg gtttgagatg aaaaagcctt accaccacgg aaacctcaaa
     2521 gaagaactca ttgctgccgg tatttctgtg ctaaaaaagg agggaaccgg gggcctgcgt
     2581 ctgcgtgaag tggctggcct tgtgggtgta agccacgctt ctctttacag gcactttaca
     2641 aataaggaag aactcctggc cgccatcgca gaacaggggt ttcgcgggct cattgaaaac
     2701 gtggagctct acaagaagcg cgccggctcc aatttcaaat cgcagttcat cgagtctggc
     2761 cgcgcttatg tggattttgc cgcgtcacac aaggaacagt ttcatatcat gttcagcgga
     2821 atcattccgg atcgaaaaaa gcacccggcc ctggcgctcg cggcggaaga atcgtttggg
     2881 cagctggtgc agattgtgga agaatgcaga gaggcaggag tgattccctc ccacagagat
     2941 agaaaagtgc acactctggc tgcctggtcc atgcttcatg gcatcgccac tctcttgatc
     3001 gaaggccaga ttccaggggg cagatcgcgc actcgagcca tcacacgatc tcttctgatt
     3061 ttgttgactg tgtgattcgc acagacgcag agccttactt tttccctccc catcccccgt
     3121 ttttattgac acttctttct attggatgcg tctctcatct gtgggtgcaa caatgaagat
     3181 tcgatcgttt ctcaacatcg cggctctgtc tctggcgttt gccttttcaa cggcgtctct
     3241 tcgcgcggag ccagtcgttc tgacggaaga cgttctgaaa agatacatgg cgatttttcc
     3301 cagctatatg agaatcactc tggagctaga cggtacctct aaaatgcggg aaggtgcagc
     3361 aaaagaagca aaggtaacag aattgataga gaagaggaat gcccttctca ctgcgaatgg
     3421 atgggaggat ttctccgagt acatggaagt ggacgcccgc gttgtgcgcg ccatgactcc
     3481 gctgggcatc ctggaaaaac tcaagaatgc atcagagcct gacagaaaga aagcggaaga
     3541 tacagttgca gcacagcttc aggattacac tccggaagaa attgctctgg taaaaaagaa
     3601 cctggtaggc cttacaaaga tgcggcgtga tgctctggct ggaaggtaaa gcgcgcataa
     3661 tcttctctcc ataataaaaa ggtcgcggat tatagaccgc ggcgacaggc gccagatgat
     3721 ccgctatcat cctgactatg cgaccatgga tcagggcctg tttcatctct cttttgtcca
     3781 gcctggcggc ccaatgcaac cagagctcca aatgcaagga ggcagatgca agttgcaacg
     3841 tccttcccct gctgctttat tcaaaggttg ccgtcgcaac caatctctac gttgtaaacg
     3901 ccacgtccgg ttcactttca gagtactcca ttgacagggg cacgggactg ctctcctccg
     3961 ttggaaccgc gacaggtatg gcaggcacga acggtgtgca ggtggatccg ctcggtcgtt
     4021 ttgtcttcgt ggccgcctcg accacgccca ggatccatcg tttcgccatc gatacgacgg
     4081 gtgcgctctc ggtccttggc acaacgacac ccaattcaac atttcctatt ggcctctgcg
     4141 ctgatcccac gggcgcctat ttattcagcg tggtcgccgg gaatggaaca gtggaaggtt
     4201 tcagtgtgag cagtaattca gtgctcacaa gtacgggaac cgcattggcc gcagcgggcg
     4261 ga
//
LOCUS       DAORFF010000336         4231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12399_length_4231_cov_3.903975, whole genome shotgun sequence.
ACCESSION   DAORFF010000336 DAORFF010000000
VERSION     DAORFF010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12399_length_4231_cov_3.903975"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2765
                     /locus_tag="PKK76_12985"
     CDS             <1..2765
                     /locus_tag="PKK76_12985"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNL69439.1"
                     /translation="PRGTQIRLGPISSIMGEILLVGITSKDGKNSPGDLRTFADWTVR
                     KQLLSVRGVADITVIGGEAKQYQILIDKNKLSAYDNSLFADNRYISFLAMKESLRGLG
                     ENTGGGFIEKEGKELSVRNLGNITDQSDLENSVIGLRGGSPVRIKDIAVVSTGARVKR
                     GDAGVNGNPAVILIIQKSPGVSTVDLTRSIATEIEKIRKANDPDILIHDKLFRQADFI
                     ERAVGNVGEAIRDGSIIILIVVFLFLWSFRTTFITLVTLPLSLIAAIVTMAALGVDLN
                     TMTMGGLAVAVGQLVDDSIVDMENIFRRLRENRNLNAPRPALEVVFHASSEVRNSIVL
                     ATLVVILVFIPLFFLGDLEGRLFRPLAFAYIVALFASLLVALTVTPVLCYYLLRNASA
                     TQNETAIVRSLKAFQRRNLNALLDRPMLSTLIVGVLFLISIGMAPFLKKDFLPSFNEG
                     VFTVFGSLPTATSLPESVARMKEVEEVLLSTKGVGQFARRTGRAEGDEHASGPHLTEI
                     EVNVEPEKAGITRAKLLADLRTKFKSLKGIDLDIGQPISHRIDHMMTGVRSEVAVFVY
                     GDDLEAIQYQAYQIRSVLSKVPGITDLLIENQTKAPELKIGINHAQASSYGITSGEIT
                     EVLEFALGGQTVGRTIENGKIFDIVLRFDDKSRAVDGLSGIIIKYLPNGTPVRLGMVA
                     EVYETEGPYEIKRENGSRRIAVQFNTQGRDLGSVVSDVNAALKTLQMPQGIRYEIGGR
                     FASRERAIRLIGIFGFCALVLLTFILYRNFKSWLLVGQILVNVPFSFIGGIGALFVLG
                     VPLSLASILGFIALTGIAARNGIMMISHFLHLMEVEGMEFSRAMVIRGALERLVPVLM
                     TAFSAVFALLPILVSGSDAAGKEILFPVALVISGGLFSSTLLDIIFTPVIFYQVMKRA
                     PNGASS"
     gene            2785..3195
                     /locus_tag="PKK76_12990"
     CDS             2785..3195
                     /locus_tag="PKK76_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69440.1"
                     /translation="MRNKLTLVLLSMLLSGFASAYAHEGHDKPDVLPPVGPHGGKYTK
                     MKNHFAEVVVQGDMAMIYILEDDIKSSPEKLSDVKASLEIPGKGNTTLKLSSMDGAFH
                     ASISIPKNVRRVYFQISAKVDGKLESGKVLYEPK"
     gene            3365..>4231
                     /locus_tag="PKK76_12995"
     CDS             3365..>4231
                     /locus_tag="PKK76_12995"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNL69441.1"
                     /translation="MALAQEKSPDMRKARAALLLARGEFVQKDRLIAANPEISVSGTR
                     GTRATSPDVSVSPSAALKGEQPVDVSPSILSGPQQQRINGYEVELSQELDLAGKSSIQ
                     KKIARVEIEKADAQLLLTRLQVRAEVRTQLTTFLMSEALAADLERQLEHLRLIKQRMG
                     PGFTDQRLGTYVGVVFQNDLARADIELRNLLLTRTNAKQTLDQMLGVTTVKVCTLPPT
                     SSRITNPTLPEESELIAGAHANSPRLRLVSLNSQQSELGSNLADIAMIPDPTFFLGAG
                     EQSIGSSKLAPSI"
BASE COUNT         1071 a         1026 c         1082 g         1052 t
ORIGIN      
        1 tgccgcgtgg aacgcagatc cgccttgggc caatcagctc catcatgggg gaaattctac
       61 ttgttggaat aacatccaaa gacggaaaaa attcgccggg tgatctgaga actttcgctg
      121 actggacggt gcgcaagcag ctcctgtccg tgcgcggcgt agctgacatt accgtcatcg
      181 gtggtgaagc caaacaatat caaattctca tcgataagaa caagctttca gcgtatgaca
      241 acagtctctt cgcggacaat cgctacatct cgtttctcgc gatgaaggaa agcctgcgcg
      301 ggctcggaga aaatactggc ggcggattca ttgagaaaga ggggaaagaa ctttctgtcc
      361 gaaatctcgg caacataacg gaccagtctg atctggaaaa ttctgtgatt ggactgcgag
      421 gtggaagtcc ggttcgcatc aaggacattg cggtggtgtc taccggcgcg cgcgtgaagc
      481 gcggtgatgc gggcgtaaac ggaaatccag ccgtgatcct gatcatacaa aaatcaccgg
      541 gcgtttctac cgttgatcta acacgaagca ttgccaccga aattgaaaaa atcagaaaag
      601 cgaatgatcc ggacatactg attcacgaca aactatttcg ccaagcggac ttcattgaac
      661 gagccgtcgg aaatgtcggc gaagcgattc gagacggttc gattatcata ctcattgttg
      721 tattcttatt cctgtggagt tttcgcacta ccttcatcac actcgttact cttcctcttt
      781 cacttattgc ggcaatcgtc accatggcgg cgctcggtgt tgacttaaat acgatgacga
      841 tgggcggtct ggccgtggcc gtggggcaac tggtagacga ttccatcgtg gacatggaaa
      901 acattttcag gcgtctgcga gagaatcgaa atcttaacgc gccgcgcccg gccctcgagg
      961 ttgtttttca cgcgtcgtcc gaagtaagaa acagcatcgt tcttgcaacg cttgttgtga
     1021 ttctcgtttt cattccactc ttttttctgg gcgatctcga aggacgattg ttccgtccac
     1081 tcgcgtttgc ttacatagtt gcactctttg catcgttgct tgttgccctg actgtaacgc
     1141 ctgttctgtg ttattacctt ctgcgcaatg catccgctac gcagaatgag actgcgattg
     1201 ttcgctctct caaggcattt caacgcagga acctgaacgc acttctggat cgtccgatgc
     1261 tgtcgaccct catcgtcggt gttctgtttc tgatttcaat tgggatggca ccatttctca
     1321 agaaagattt cttgcccagt ttcaacgaag gagtgttcac ggttttcggc tcgctgccca
     1381 cagccacttc gctgcccgaa tccgtggcgc gcatgaaaga agttgaggaa gtcctgcttt
     1441 cgacgaaagg tgtgggacaa tttgcgcgcc gcacaggacg cgcagaaggc gatgagcacg
     1501 cgagcggccc gcatctaacg gagatcgaag tgaatgtgga accggaaaag gcaggaataa
     1561 cccgcgcaaa acttcttgcg gatcttagaa caaaattcaa atcactgaaa ggaatcgatc
     1621 tcgatatcgg acaaccgatc tcacatcgaa tcgatcacat gatgaccggc gttcgttcgg
     1681 aagtcgcagt ctttgtttac ggtgacgatc ttgaagcaat ccaatatcaa gcgtaccaga
     1741 ttcgtagcgt cctgagcaaa gttccgggca tcacagattt actcatcgag aatcaaacta
     1801 aagcgcccga actcaagatt ggaatcaatc acgcgcaggc gagttcctat ggtattacaa
     1861 gcggagaaat caccgaggtt ctggagtttg ccctcggtgg ccagacagtt ggaagaacca
     1921 ttgagaatgg aaaaatcttc gacatcgtgc tcagatttga tgacaaatcg cgcgcagtag
     1981 atggtctctc tggaatcatc atcaagtacc tgccaaacgg aacgccggtt cggcttggaa
     2041 tggttgcaga agtttatgaa acggaaggcc cgtatgaaat aaaaagggaa aacggcagca
     2101 ggcgcattgc ggttcaattc aatactcaag ggcgcgatct gggctctgtc gtttccgatg
     2161 taaatgccgc cctcaagaca ttgcagatgc cgcagggcat tcgctatgag attggcggac
     2221 gcttcgcaag tcgggaacga gcgattcgac tcatcggaat ttttggattc tgcgcacttg
     2281 tgcttctgac cttcatcctg taccgaaatt ttaagtcctg gctccttgtt ggacaaatcc
     2341 tcgtgaatgt gccattctcg ttcatcggtg gtatcggcgc actgttcgta ttgggtgttc
     2401 ctctctcact tgcaagcata ttgggattca tcgcgttaac aggcattgct gcaagaaatg
     2461 gaatcatgat gatctctcat ttcctgcatt tgatggaagt agagggcatg gaattctcaa
     2521 gggcaatggt gattcgtggt gcacttgaac ggcttgtacc tgttctaatg accgctttct
     2581 cagcggtctt tgcgctcctc cctattcttg tttcagggag cgacgcggcc gggaaggaaa
     2641 ttctcttccc ggtggccctg gttatctccg gtggattatt cagctcaact ctactcgata
     2701 ttattttcac gccggtaata ttctaccagg tcatgaaaag agcgcctaac ggtgcaagtt
     2761 cataaaaaac aaaagaggtt aaacatgaga aacaaactaa cattggtgct gctctcaatg
     2821 ctcctgagcg gatttgcttc cgcttacgcg catgaaggcc atgacaaacc cgatgttctt
     2881 ccgccggttg gtccgcacgg tgggaagtac acaaagatga agaatcactt cgcagaagtg
     2941 gtagttcaag gtgatatggc catgatctac attcttgaag atgatataaa gagttctccc
     3001 gagaaactga gtgatgtgaa agcatcgctt gaaattccgg gaaaaggaaa caccaccctt
     3061 aagctcagtt ccatggacgg agctttccat gcgagcattt cgattccaaa gaatgtcagg
     3121 cgcgtgtatt ttcaaatcag cgcgaaagtc gacgggaagc tggaatcggg gaaagttcta
     3181 tacgagccca agtgataaga acggcgatcc ccaacctgtg tgaggatcgc cgttttttga
     3241 ggagcattat atgaaaacta ttcgctgtcg attcattctt gtaagtatca ttctacttcc
     3301 gctgcttttg ggtgttcttc ctgtgacagg aaatgcgccg gagtgtctca cactgaacga
     3361 ggccatggcg ctcgcgcagg aaaaaagtcc tgatatgaga aaagcgcgcg cggcgttgtt
     3421 gctagccagg ggagaattcg ttcagaaaga ccgtttgatt gcagcgaatc ctgagatttc
     3481 tgtgagtggc acacgcggta cgcgcgcaac ttcgccggac gtttctgttt ctccgtcagc
     3541 ggctcttaag ggtgagcaac ccgttgatgt ttcccccagc attttgtcgg gaccacagca
     3601 acaacggatc aacggatatg aagttgaatt gagtcaagaa ctggacctcg ccggaaagag
     3661 ttccattcaa aagaaaattg cgcgagttga gatcgagaag gctgatgcac aactcctcct
     3721 tacacgattg caagtgcgtg cggaagttag aacgcaactt acaactttcc ttatgtctga
     3781 ggccctggca gcggatttag aacggcagct cgaacatttg cgtttgatca agcaaagaat
     3841 ggggccaggg tttactgatc agagacttgg aacctatgtc ggtgtcgtgt ttcaaaatga
     3901 tcttgccaga gcggatattg aattacgcaa cctcctgcta acccgaacga acgcgaaaca
     3961 aactctggat caaatgcttg gcgtgacgac agtgaaggtc tgcacacttc ccccaacatc
     4021 ctctagaatc acgaacccga cgctgccgga agaaagtgaa ctaatcgcgg gggctcacgc
     4081 gaacagtccc cggcttcgcc ttgtcagttt aaattcgcaa caaagtgaac ttggcagcaa
     4141 cctcgccgac atagccatga tacctgaccc gacatttttt ctcggtgctg gcgaacaatc
     4201 gattggatcg agtaagctgg ctccctccat c
//
LOCUS       DAORFF010000337         4213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12493_length_4213_cov_2.911255, whole genome shotgun sequence.
ACCESSION   DAORFF010000337 DAORFF010000000
VERSION     DAORFF010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12493_length_4213_cov_2.911255"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /locus_tag="PKK76_13000"
     CDS             <1..1855
                     /locus_tag="PKK76_13000"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNL69442.1"
                     /translation="RGMHTSDVLGGLKPVRDRELEWVDGPITRAVRNGHYLLIRGLEA
                     AGPELVEKLNMLLDDARALLLPPEAGEREPLLLKENSVIFAVKVFRNTKSITTISRAF
                     RNRFSSVIVGATQDPVSLEDIVLSALDSDESARDLAARMVQFHLKIRDHAEKREIGSA
                     NIQPYMYGLTNLHRWASLLVGSNPESGEDLKAIVIRSAGISYLNEISDPMEREKMYQA
                     LERILSGLPLDELSQNADALKKKHLIKRTTFQKKIDWDPSQHFREANTGRAQRKLTGQ
                     ELKKGVRINTPETGGNTKEGPDAWYGQDTQGNRGQGEPGHGGGGWGYRTEELYQEFLK
                     KRRALWEYNIGVSLQDFHEVFGSEIERVVIDFDRLLDPRSDIHRRYQNHGSRVDGRRY
                     LAYISGKGDGRVFDKTTVVLERDRLKGVEIIFAVNKGRRIFNFEYSIATLVSILSSTI
                     ILSNHKVPVAACGYSDLKNQKKDIDLMWFKNLDDPYTNQEEEHLFSGLARDWHGDTVP
                     EADVLVSLADSFSAAATTRILVMLSDFRGSRAKMKLEDTVASKESVDLKQMTEQMEKR
                     DIHFLGVGLGPKSLAEYFFPEHLSVGGENYANLPILLSNKLSEMIHRYHRS"
     gene            1852..2517
                     /locus_tag="PKK76_13005"
     CDS             1852..2517
                     /locus_tag="PKK76_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNL69443.1"
                     /translation="MKKPLASKKKSKPVTTKKAINARSKPSQAGRLSRNFARDVYVHP
                     QATVMGDVTLGQGVSIWPGAVLRGDMNSIRIGRMVNIQDNCTLHVDSSLGLEIGEYTL
                     VGHNCMIHSCTIGRGCLIGIGSVILEGAVIGDGAMITAGCLVRGGRKIPPFSLVVSKN
                     GELVVYEKKARTLLALAGCIEYSELARRAQKGKFGPFSVDEEKQFYAEAQIVYAQLFG
                     EVK"
     gene            2514..3713
                     /locus_tag="PKK76_13010"
     CDS             2514..3713
                     /locus_tag="PKK76_13010"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNL69444.1"
                     /translation="MNGNIEQAIEDIRHGRMIILVDSEARENEGDLIIAAEYADAQAV
                     NFMAVHGRGLICVPLDEETAVRLELGPMVRRNTDQFRTNFTVSVDAAHGTTTGISAAD
                     RAATIKVLADSTSRPEDLIRPGHIFPLIAAKGGSLRRAGHTEASTDLARLAGLRPVSV
                     ICEIMNEDGTMARLPDLQIFAERHGLKIYTIEDLIRYRRAHDTLIRKEVEARIPTDFG
                     EFKVNAYSTTVDDKIHLALIHGDVKGKENVPVRVHSECLTGDVFHSLRCDCGEQLARA
                     LSFISRQECGVLLYMRQEGRGIGLLNKLKAYELQDTGLDTVEANEKLGFAADLRDYGI
                     GAQMLSSLGITSMQLLTNNPRKVVGLEGHGLVITGRIPIAVEPGDENRKYLTTKKNRL
                     GHLLDTV"
     gene            complement(3710..4006)
                     /locus_tag="PKK76_13015"
     CDS             complement(3710..4006)
                     /locus_tag="PKK76_13015"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNL69445.1"
                     /translation="MTAIELRSAPGRLEVFSPQTSEGAYRVVIIDNDVNTYEQVIQIC
                     MKALGIHYRDALEIAIAVDQNGRAEVFEGSQADAEAVASVIRTIGIEVLVVPAA"
BASE COUNT         1088 a          997 c         1146 g          982 t
ORIGIN      
        1 tagaggaatg cacacctctg atgttctcgg tggtcttaag ccggttcgcg accgtgaact
       61 ggaatgggtg gacggaccaa tcaccagagc agtgcgaaac ggccattatc tgctgattcg
      121 cggactggaa gccgcaggac cggagctcgt agaaaagttg aacatgctcc tggatgatgc
      181 aagagctctt ctccttccac cggaagcagg cgaaagggaa cctctcctcc tgaaagagaa
      241 ctctgtaatt tttgccgtta aggtatttag aaatactaaa agtattacaa ctatttccag
      301 agcatttcga aatcgcttta gctcagtgat cgttggcgcg actcaggatc cggtcagcct
      361 tgaagatatt gtactgtctg ctctcgattc ggatgagagt gcacgtgatc tggccgcacg
      421 catggttcag tttcatttga agattcgtga tcatgcagaa aagcgcgaga tcggttctgc
      481 caatattcaa ccttatatgt atggccttac caatctccac agatgggcca gtctgcttgt
      541 cggaagcaat cctgaaagtg gggaagatct aaaggcgatc gtcattcgat ctgcgggtat
      601 ttcctatctc aacgaaatca gcgatccaat ggaacgagag aagatgtatc aggctctgga
      661 acggattctt tccggtcttc cactcgacga gctcagccag aatgctgatg cgttaaaaaa
      721 aaaacacctg attaagcgaa ccacctttca gaagaaaatc gactgggatc catctcagca
      781 ttttcgcgaa gcaaatacag gccgcgccca gcgcaagcta accgggcaag agctgaagaa
      841 gggtgttcgc atcaatacgc cggagaccgg tggaaacaca aaagaaggtc cggacgcatg
      901 gtacggacag gacacccagg gaaatcgtgg gcagggcgaa cccggacacg gtggtggcgg
      961 atggggctat cgcacagaag aactatatca agaattcctg aagaagaggc gcgcgctctg
     1021 ggaatacaac attggcgttt ctctgcagga ctttcatgaa gtctttgggt ctgagataga
     1081 aagggtggtg attgattttg atcgattgct cgaccccagg tccgacattc atcgtcgcta
     1141 ccagaatcac ggatctcgcg tggacggtag acgttatctg gcatacatat caggaaaagg
     1201 ggacggccgg gtcttcgata agaccaccgt tgtgctcgaa agagatcgac tcaagggggt
     1261 tgaaatcatt ttcgccgtca acaaaggaag gcgcattttc aatttcgaat actcgattgc
     1321 cacactcgtt tctattttaa gcagcacgat cattctctcc aatcacaagg tgccagtggc
     1381 tgcctgcggt tattctgatc ttaagaatca gaagaaagac attgatctga tgtggtttaa
     1441 gaatctggat gatccttata cgaatcaaga agaggagcat ctgttttctg gactggcgcg
     1501 agactggcac ggagataccg ttcccgaagc agacgtgctt gtatcgctcg cagacagttt
     1561 ttcagccgct gctaccacgc gcattctggt gatgctttct gacttccgcg ggtctcgtgc
     1621 gaagatgaaa ctggaagata cggttgcgtc taaagaatct gtggatttga agcagatgac
     1681 ggagcagatg gagaaacgcg acattcattt cctgggtgtt ggtcttggtc caaagtcact
     1741 ggccgagtat ttctttccgg aacacctcag cgtgggtgga gagaactacg cgaacctgcc
     1801 cattttactt tccaataagc tttcagaaat gatccatcgg tatcatagat catgaaaaag
     1861 cctctggcct cgaaaaagaa atcgaagccg gtcaccacca aaaaagcaat caatgcgcgg
     1921 agcaaacctt cacaggcagg ccggctttct cggaactttg ctcgggacgt gtatgttcat
     1981 ccccaggcga ccgtgatggg agatgtcacc cttggacaag gggtgtcgat ctggcccgga
     2041 gcagtccttc gcggagacat gaacagcata cgaattggca ggatggtgaa cattcaggac
     2101 aactgcactc ttcatgtaga ctcttcgctt ggtctggaaa taggcgaata cactctagtc
     2161 ggtcacaatt gtatgattca ttcatgcacg atcggaagag gctgcctgat tggaatcgga
     2221 tctgtcatcc tggaaggagc ggtcattgga gacggcgcca tgatcactgc cggttgtctg
     2281 gtgcgtggag gacgcaagat ccctccgttt tcccttgtcg tttcaaagaa tggagagcta
     2341 gttgtatatg agaagaaggc gcgcactctc cttgcactcg ccgggtgcat tgaatatagc
     2401 gaactggccc ggcgcgcaca gaagggaaag ttcggccctt tttccgttga tgaagaaaag
     2461 caattttatg cagaggcaca gatcgtctac gcgcagttat tcggagaagt gaaatgaacg
     2521 gaaatataga acaggcaata gaagacattc gacacggccg catgatcatc cttgtggatt
     2581 cggaggcacg cgaaaacgag ggagacctca tcatcgcggc agagtatgcg gatgcgcagg
     2641 ctgtgaactt catggccgtt cacggccgag gcctgatttg cgttcccctg gacgaagaaa
     2701 cagcagtgcg tctagagctc ggacccatgg ttcgcagaaa tacagatcaa ttcagaacca
     2761 attttactgt aagcgtggat gcggcgcacg gaactacaac tggaatttca gctgcggatc
     2821 gcgcggccac gatcaaggtg cttgcggaca gtacgtctcg tcccgaggat ctgattcggc
     2881 ccgggcatat tttcccactg attgcggcaa aaggtgggtc tttgagaaga gccgggcata
     2941 cagaagcctc tacggatctt gcaaggcttg ccggactcag gcctgtatct gtgatctgcg
     3001 aaatcatgaa tgaagatgga accatggctc gtcttccgga tctgcagatt ttcgcagaac
     3061 gccatggcct gaagatttat acaattgaag atttgattcg ctatcgccgt gcgcacgaca
     3121 ctctgattcg caaagaagtg gaagcaagaa ttccaacaga ttttggcgaa ttcaaagtga
     3181 acgcgtactc cacaaccgta gatgataaaa ttcatttagc attgatccac ggagatgtaa
     3241 aagggaagga aaatgttcca gtgcgcgtgc acagcgagtg tcttaccgga gatgtgtttc
     3301 attctctccg ctgcgattgt ggtgaacaac ttgcgcgagc gttgtctttt atttcacgtc
     3361 aggaatgcgg cgttctactc tacatgagac aggaaggtcg ggggatcggg cttctcaata
     3421 aactgaaagc ctacgaattg caggacacgg gcctcgatac tgtggaagca aacgagaagc
     3481 tggggtttgc cgctgacctt cgcgactacg gaattggcgc acaaatgctt tcttctctgg
     3541 gaatcacaag catgcagctt ctgactaaca atccgcgcaa agtggtcggg cttgaagggc
     3601 atggtctggt tatcacaggt agaatcccga tcgcagtgga gcctggagat gaaaacagaa
     3661 agtatctaac caccaagaag aatagactgg gccatctgct cgatacagtc taggcggcgg
     3721 gcacgaccag tacttcgatt ccgatggtgc gaatgacaga agccacagct tccgcatccg
     3781 cttgcgaccc ttcgaaaact tctgctctgc cattctgatc caccgcaatg gcgatttcca
     3841 gcgcgtctct gtaatggatt ccgagcgctt tcatgcagat ctgaatgacc tgctcatacg
     3901 tatttacgtc attgtcgatg atcacgactc tataggctcc ttcgcttgtt tgaggcgaaa
     3961 agacctccag gcgtcccggg gcggagcgaa gctcgattgc ggtcacttgt tttttgaatt
     4021 agatcctcga ttctgcgcaa gaaattaatt gattggcccg gcaccggctg gttcatgaag
     4081 atccggctga gagacgaagg cgaactggtc gggatgatgg acgacgaagc gtatcaggca
     4141 atgctgcgga gcctcggttg atgcgctatc tgccgctgac cgacgacgac cgccagcaga
     4201 tgctcgcggc gat
//
LOCUS       DAORFF010000338         4200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12548_length_4200_cov_3.929071, whole genome shotgun sequence.
ACCESSION   DAORFF010000338 DAORFF010000000
VERSION     DAORFF010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12548_length_4200_cov_3.929071"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PKK76_13020"
     CDS             complement(<1..1087)
                     /locus_tag="PKK76_13020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNL69446.1"
                     /translation="MSDELLKQIETKTSDLLKQVTETLAKRSEEKGQVSVSKMDENQL
                     VHYNLSYSTAEKRISDHFTKYAFAKENGTGELEQDMARLFAGEAAHRLRSELASRKDE
                     YGASDALLNDLFSPDMNKKIQELTSRDRYNKVGDAALTGSFGALATEDLKDIRDTYKK
                     IAEQVVMPHAEHVHRNDDFVPDDILQSLLDNGAFGLSIPEEYGGYQDTFGNTGMMIVT
                     EELSRAGLSIAGSLITRPEILSKAILKGGTPEQKTKWLGALATGEKMCAVAATEPDYG
                     SDVAGFKVTADPVEGGWVLNGVKTWCTFAGYADSLMVLARTEKDPGLKHKGLSILIAE
                     KPRDKGHKFDFAQPSGGKCTGSAIPTIG"
     gene            1141..2184
                     /gene="hemH"
                     /locus_tag="PKK76_13025"
     CDS             1141..2184
                     /gene="hemH"
                     /locus_tag="PKK76_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNL69447.1"
                     /translation="MARTSDRTGILIINLGTPDSPSTHHVRRYLREFLSDPRVIDIPG
                     LLRFFLLNFIILPLRPARSAEAYRKIWTNEGSPLLIHTRDLSLALARHLPRVSVKFAM
                     RYGSPSIPDVMKEFAKEGVRKIKVLPLYPQYAAASTASTSARVYECAASMNDPLEVEM
                     LGPFFDHDGFLDATAHLARPFLASAKGAHVLFSYHGVPERQIDRSVRSGLSHADQEHC
                     SFGDCCHSPGDRMALCYRAQCFSTTKGLVSRLQLKQEDYSIGFQSRLGRTPWVRPYTD
                     ELIQTLPARGVKHLAVICPSFVSDCLETLEEIQIRAKEDFLRAGGESFVYIPCLNSDE
                     TWVKAVARMVEGE"
     gene            complement(2185..2859)
                     /locus_tag="PKK76_13030"
     CDS             complement(2185..2859)
                     /locus_tag="PKK76_13030"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HNL69448.1"
                     /translation="MVLSTQGLISSIDRLADHKEDFLRPKPKSRACQAAMRCEKCHLL
                     PSNCICAQIPSIRTRMRLTLILHALEARRSTNTGHLAALALSNSRVEIHGRAGQAIND
                     SFEVTAGMKPLFLFPDGSEELRPEMANDGDVELIVPDASWAQAVRMSRRIHGLSGVPH
                     VRLPRVTPRKILRKSLEGRISTFEAISHAVQILEGEEAGASMLTLFDAYASALLRERG
                     RRSRFD"
     gene            2858..3880
                     /locus_tag="PKK76_13035"
     CDS             2858..3880
                     /locus_tag="PKK76_13035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNL69449.1"
                     /translation="MIRLTLLIALTLPVLNCATMTIHTGTGNLYGREGQKEEGNYFEP
                     ATKRDASSQMPACKTEPPVAGNDPHNTAVAPEKTPEKTPEKVSDRSAPGYDDKAKDEE
                     ASYFNNQAENPPVVADNSTGEESGTASWYGRDFDGKATASGEPFDSRKLTAAHRTLPL
                     GTVILVKNTENNREALVTVNDRGPFVKGRIVDLSEYGAEMLGYKEKGLTTVSIRVVRR
                     GAMTEKGPGVTSAAFNDADHAQGNGPESVKPQVVTRPKVQAPANIREEKALGKYSVQV
                     GLFSEYDNADKLGKYLTSYNQPVNIFQRGTSYVVKVGEFADKNQAEQLKTRLSSDGYT
                     AFISAP"
     gene            complement(3915..>4200)
                     /locus_tag="PKK76_13040"
     CDS             complement(3915..>4200)
                     /locus_tag="PKK76_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69450.1"
                     /translation="LEILDIIFKDDHNARLLGPDGTWKRLRPEGGGEGFSSQRYFRDQ
                     ARKNFEQKEKERESQIVFQPAGSPNTPVLSRLIPVDHEAGERASQSDSGN"
BASE COUNT          976 a         1070 c         1069 g         1085 t
ORIGIN      
        1 aaccaatcgt tgggatagca gatcctgtac actttccgcc tgagggctgt gcgaaatcga
       61 acttatgacc tttgtcgcga ggcttttctg caataagaat ggaaagacct ttgtgtttga
      121 ggcccggatc cttttcagtg cgagcaagaa ccatgagaga gtctgcgtat cctgcaaacg
      181 tgcaccatgt tttcacaccg ttgagcaccc agccgccttc caccggatct gcagttactt
      241 tgaatccagc tacgtcagat ccgtagtccg gttcggtagc tgcaaccgcg cacatctttt
      301 ctccagtggc gagagctccc agccactttg ttttctgctc gggtgttccg cctttcagaa
      361 tcgccttgct taagatttca gggcgagtga tcaagctgcc tgcgatggaa agtccggcgc
      421 gcgaaagctc ttctgtaaca atcatcatgc ctgtattgcc aaatgtgtcc tgatatccgc
      481 cgtattcctc tggaatagac agaccaaacg ctccattgtc caggaggctt tgcagaatat
      541 cgtccggcac gaaatcgtca ttgcgatgaa catgttctgc atgaggcatc acgacctgtt
      601 ctgcgatctt cttgtatgtg tcgcgaatgt ctttgagatc ttccgtggcc agtgctccga
      661 aggatcccgt gagagctgcg tctcccactt tgttgtatcg atcgcgtgaa gtgagttcct
      721 gaatcttctt gttcatgtcc gggctaaaca gatcgttgag aagtgcatcg cttgcgccgt
      781 attcgtcttt gcggctggca agttctgatc tgaggcgatg agccgcttct cctgcgaaga
      841 ggcgtgccat atcctgctcc agttcgcccg ttccattctc tttcgcgaag gcgtatttcg
      901 tgaaatgatc ggagattctt ttttcagcag tcgagtagga gagattgtag tgaaccagct
      961 ggttctcatc catcttagag acggatacct gacctttttc ttcggatctc tttgcgagag
     1021 tttccgttac ctgttttaga aggtcgcttg ttttggtttc aatttgtttc agcaattcgt
     1081 cagacataga cccacttttt gattttgaat tttggcggcc aatcaaatta aggtaagact
     1141 atggcccgaa cctcagacag aactggcatt ctgattatca atctcggcac tccggatagc
     1201 ccctccacgc accatgttcg tcggtatctg cgcgagtttt taagcgatcc cagagtcata
     1261 gacatccctg gactgttgcg attctttcta ttaaacttca tcatactgcc cctcaggccc
     1321 gctcgttctg cggaggctta caggaagatc tggacgaacg aaggttctcc tcttttgatt
     1381 cacactcgcg acctgtctct tgctcttgcg cgtcatttgc cccgcgtaag cgtgaagttc
     1441 gccatgcgat acggtagccc ttccattccg gatgtcatga aagaattcgc gaaggaaggc
     1501 gtacgtaaga tcaaagtgct tcctctctat ccgcagtacg cggccgcttc tactgcttcc
     1561 acatccgcgc gcgtatacga atgtgccgcc tccatgaacg atcctttaga agtagaaatg
     1621 ctcgggccct tttttgatca tgatggattt ctggatgcaa cggcacacct ggctcgtccc
     1681 tttctggcca gtgcgaaggg agcccatgtt ctattcagct accacggagt gccagaaaga
     1741 caaattgatc gttctgtccg ttcaggttta agccacgcag atcaagagca ttgttccttc
     1801 ggagattgtt gtcactctcc tggagatcgc atggcactgt gctatcgcgc tcaatgtttt
     1861 agcacgacga agggactcgt gtccaggctg cagctgaagc aggaagacta cagcattggt
     1921 tttcaatctc gcctcggaag aactccgtgg gttcgaccct acacagatga attgatccag
     1981 acgcttccag cgcgcggagt caaacatctg gcggtgattt gtccttcgtt tgtttctgat
     2041 tgtcttgaga ctctggaaga aattcaaatt cgcgcaaaag aagatttctt gcgcgcgggt
     2101 ggagaatcgt ttgtgtatat cccgtgcctg aactcggatg aaacctgggt gaaggcagtg
     2161 gccagaatgg ttgagggaga atagtcagtc gaatctgctt cttcttccgc gctccctgag
     2221 cagagcggac gcgtatgcgt caaatagagt gagcatggat gcgcctgctt cttcgccctc
     2281 tagaatttga actgcatgtg aaatcgcttc aaacgtggaa atgcgtccct ccagagactt
     2341 tctcaaaatc tttctggggg tgactcgcgg aagtcgtacg tgtggcactc cagagagtcc
     2401 gtgaatgcgt ctgctcattc gaactgcctg agcccagctt gcatctggaa caatgagctc
     2461 cacatctccg tcattcgcca tttcaggccg caattcttct gatccatccg gaaaaagaaa
     2521 cagaggcttc attccggcag tgacctcaaa agaatcattg attgcctggc ctgcgcggcc
     2581 atgaatttct acgcgcgaat tggaaagtgc cagtgctgcc agatgccccg tgttcgtgga
     2641 acgtctggct tccagggcat gcaggatcaa ggtgagccgc attcgagtgc gaatagaggg
     2701 aatctgcgcg cagatgcaat tagacgggag gaggtggcat ttctcgcatc tcatggcagc
     2761 ctggcaggct cgcgattttg gttttggacg caagaaatct tctttgtggt ctgcgagtcg
     2821 gtcgatactg gaaatgagtc cctgtgtgga gagaaccatg atccgtctta ccctactaat
     2881 tgcactgacg cttccagtat tgaattgcgc cacaatgacc attcataccg gaacaggaaa
     2941 tctgtacggt cgcgaaggcc agaaagaaga gggcaattat ttcgagcctg caaccaaacg
     3001 ggatgcatcc tcgcaaatgc ctgcatgcaa gacagaacca cctgtagctg gaaacgatcc
     3061 gcacaatact gctgtggcac cagagaagac tccggaaaaa acacctgaga aggtaagtga
     3121 tcgttctgcg ccagggtatg acgacaaggc taaggatgaa gaagctagtt acttcaacaa
     3181 tcaagcagag aatccaccgg ttgtggcaga caattctact ggagaagagt caggaactgc
     3241 atcctggtat ggtcgtgact ttgacggcaa agcaacggca agtggtgaac catttgattc
     3301 gcgcaagctc accgccgctc atcgcacttt gcctctgggc acagtcattc ttgtaaagaa
     3361 cactgaaaac aatcgcgaag cactcgtgac tgtaaacgat cgcggacctt tcgtaaaagg
     3421 gcgcattgtg gatcttagcg aatatggtgc agaaatgctc ggttacaaag agaaaggact
     3481 gaccactgtt tcgattcgag tggtgcgtcg tggagccatg actgagaaag gtccaggcgt
     3541 tacgtctgcc gcattcaatg atgcagatca tgcgcaagga aacggaccgg aaagcgtcaa
     3601 accacaggtt gtaacgcgcc ctaaggttca ggcacctgcg aacatccgcg aagaaaaagc
     3661 gcttggtaag tattcagtgc aggttggact cttttcagag tatgacaatg cagacaagct
     3721 tggtaaatat cttaccagct acaaccagcc ggtgaatatt tttcagagag gcacaagcta
     3781 cgttgtgaag gtaggggagt tcgcggataa aaaccaggca gaacaactca aaaccaggct
     3841 gtcatctgac ggctacactg cattcatcag cgcgccgtaa ggcgcgcctt cgccctgctc
     3901 cgcgcccctc gtgttcagtt ccctgaatcg ctctgggatg ctctttcccc tgcttcgtga
     3961 tccactggaa tgagcctgga aagaacgggc gtgttcgggc ttcctgctgg ctgaaaaaca
     4021 atctggctct cgcgttcctt ttccttttgc tcaaaattct ttcttgcctg gtcgcgaaag
     4081 tacctttgcg aagagaaacc ttctccgcca ccttctggcc tgagtctctt ccatgtgcca
     4141 tccggtccaa gtagtctggc gttgtgatca tctttgaaga tgatatccag gatttccagg
//
LOCUS       DAORFF010000339         4195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12581_length_4195_cov_3.048792, whole genome shotgun sequence.
ACCESSION   DAORFF010000339 DAORFF010000000
VERSION     DAORFF010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12581_length_4195_cov_3.048792"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PKK76_13045"
     CDS             <1..869
                     /locus_tag="PKK76_13045"
                     /inference="COORDINATES: protein motif:HMM:NF025709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4401 domain-containing protein"
                     /protein_id="HNL69451.1"
                     /translation="VIYGFLLMGAACVLSRIGRNVAMEQGALAASFSGQVLFLMGVYE
                     LLGHPRESGVVFYVGSAMEIILVFANADRLHRFLVTGLALTFFFVGWGFANHGLSGIG
                     LCIVWAVGGVLFWLISGDGIPAFWFYFLRPAGVASFIVLPLLAFSEWQAPSGDFAYTH
                     QVLTGIATVLLIVFLYVRLANQRAFLVVSCLFVLALAWITSSSPAIMLAAFMIGTAFH
                     ARSLPLLGYSMILLGLGVFQFYYEMGVSLLIKSYILMGSGLVLAAAWMVLRYLVGPES
                     SEEEKDKSGVEV"
     gene            866..1378
                     /locus_tag="PKK76_13050"
     CDS             866..1378
                     /locus_tag="PKK76_13050"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HNL69452.1"
                     /translation="MNRIDLVLRLLMVALGLSILFLVNQSILKKEDTIRNGAVILLPL
                     APVDPRSLMQGDYMRLNYKLSIVRSVEESAGLVQDGILAVSMEKQIAVSDRRIQSLSE
                     LRKGELPLRFRQRGRVLKFGAESFFFQEGTGEDYAKARFGKLRVDSDGNPILVALCDA
                     SGKEMGFALK"
     gene            1380..2057
                     /locus_tag="PKK76_13055"
     CDS             1380..2057
                     /locus_tag="PKK76_13055"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNL69453.1"
                     /translation="MAAKKIQTTRKKIETEKGSISVCIRRPSSFNVCLIIANGAGAPM
                     DSPFIRHFCDSLAEDGVMTVSFNFPYQEKKRKIPDRQAVLEQTYLSVIDLVARESKLA
                     LSHIVIGGKSMGGRIATHIAPRANVGGLVLLGYPLHAVGKPDVVRDEHLYSIHVPCLF
                     VSGDRDSLCTLALMKKVVSKMKRAAIFVVEDGDHSFKIRKNPARTQEHVFADVREQIL
                     LFLKKFK"
     gene            2054..2710
                     /locus_tag="PKK76_13060"
     CDS             2054..2710
                     /locus_tag="PKK76_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69454.1"
                     /translation="MNARLCRSGLKVGIALVIIFICFAATPQPGREEAAESQPLATQL
                     KVKKVEWNLDTFCAHVWIENQSKSPVDRLIIEIGADSLRPVRTTWNRSGSEAVLAPHA
                     ERSFEICDSSLASRKEQLTARVIMASAVIDLAKYSDLCDYIRRCEVNPEAKRLLANPE
                     ACEKMFAGLERSSQTMAFIPIIEDCARKTACSELETFKCFALSATRSIPTKSDSSVSP
                     "
     gene            2718..3632
                     /locus_tag="PKK76_13065"
     CDS             2718..3632
                     /locus_tag="PKK76_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69455.1"
                     /translation="MSLIVITSYYNPEHYRSRFENFGRFVAGLESAGIRWMVGELAFG
                     EDDFELPDHPSIVRMRGGDVMWQKERILNLLLDQLPSDAKYVAWMDCDLLFENPDWAR
                     ETERNLKSDAVVQLFDYVVRLPRGHASYRGEGEGWEGIGSIQRRSPNVLLGGDFAAHG
                     HTGFAWAAHRSILEKVRLYDACIAGSGDHMMAHAFYGDWHSACVDRILCRNSFHVSHF
                     ERWCRAVYPLVRAKVGFTRGAIYHLWHGETTDRRYVLRNRELAAFGFDPVRDIRVGPE
                     GLWEWTGKKSGLPDWARSYFKQRKEDAC"
     gene            3626..4111
                     /locus_tag="PKK76_13070"
     CDS             3626..4111
                     /locus_tag="PKK76_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69456.1"
                     /translation="MLNLLKWPFLLLWRLLTLGAGRGRIGEVADRIARHGDRFANARQ
                     NEKASIARQYSGRARNAFTVGGAESLEEDFLVAVGLKAARQVREAVESDANDLTRDSW
                     LRNSSSNSRSSESASSGFVSSSSSSDSSSSESSVEAGGGSFGGGGARQIRRRAGFLSH
                     N"
BASE COUNT          941 a          961 c         1181 g         1112 t
ORIGIN      
        1 tggtgatcta cggtttcctt ctcatgggag ccgcatgcgt tttgtctcgc atcggacgaa
       61 atgtcgcaat ggaacagggt gcacttgccg caagcttctc cgggcaggtt ttgttcttga
      121 tgggtgtcta tgagctgctg ggccatcctc gagaatccgg agttgtgttc tatgttggaa
      181 gtgcaatgga aatcattctt gtattcgcaa acgcagacag gctgcatcga tttcttgtca
      241 ctggtcttgc cctcactttc tttttcgtcg gctgggggtt tgcgaaccat ggcctatccg
      301 ggatcggcct ttgcattgtc tgggccgtgg gtggagtttt gttctggttg atttccgggg
      361 atggaattcc ggctttctgg ttttatttcc tgaggccagc aggagtcgct tcgttcattg
      421 ttttgccgtt gcttgcattt tcagagtggc aggccccgtc aggcgacttt gcttacactc
      481 atcaggtttt gactggaatt gccaccgtgc ttctcattgt gtttttatat gtgaggcttg
      541 cgaatcaaag agcgtttctc gtagtctcct gcctctttgt tcttgcactt gcctggatca
      601 catcttcttc accggcgatc atgcttgcgg cattcatgat cggcactgca tttcatgcga
      661 ggtcgctccc acttctcgga tattcaatga tcctgctcgg gctgggagtc tttcagtttt
      721 actacgagat gggagtgtcg ctgttgatta agtcttatat cctgatggga agcgggctag
      781 ttctcgccgc ggcctggatg gtattgcgat acctcgtcgg gccggaatca tccgaggaag
      841 aaaaagataa gtcaggagtg gaagtatgaa tcgcatcgac cttgtccttc gcctgcttat
      901 ggtggctctg ggtctttcga ttctatttct cgtgaatcaa tcgattctga agaaggaaga
      961 cacgattcga aacggggcgg tgatcttgct tccccttgca ccggtggatc cgcgttctct
     1021 catgcaaggc gattatatga ggctcaacta caagctcagt atagttcggt ctgtcgagga
     1081 aagcgcagga ctggtgcagg acggaattct tgcagtatcc atggaaaagc aaatcgcggt
     1141 ttcggacaga agaatacaaa gcctgtctga attgcgcaag ggagagcttc ctcttcgctt
     1201 caggcaaaga ggtcgcgtgc tgaaattcgg tgcggagtct tttttctttc aggaaggaac
     1261 aggcgaggat tacgcgaagg caagattcgg aaagttgcgt gtggattcag acggcaatcc
     1321 aattctagtg gctctttgtg atgcgtctgg aaaagaaatg gggtttgcac tgaaataaga
     1381 tggctgcgaa aaagatccaa acaacccgaa agaaaataga aacagagaag ggatctatct
     1441 ctgtttgcat tcgtcgccca tcttccttca acgtctgttt gatcatcgcc aacggtgctg
     1501 gcgctcccat ggatagtcct ttcatccggc acttttgcga ttcgctcgca gaagatggag
     1561 tgatgactgt atctttcaat tttccttatc aggaaaagaa acgcaaaata ccggatcggc
     1621 aggcagtact ggaacagacg tatctatccg tcattgattt ggtcgccagg gagtcaaagc
     1681 tcgcgctctc gcacattgtg atcggcggca agtccatggg aggtagaatc gcaacgcata
     1741 tcgcgcctcg cgcgaatgtg ggaggcctgg tgctccttgg atatccgctc cacgcagtcg
     1801 gtaagcccga cgtggtgagg gacgagcatc tctattccat ccacgttccc tgtctgtttg
     1861 tttccggcga tcgagattca ctgtgtactc tggcgctcat gaagaaggta gtttcaaaga
     1921 tgaagcgcgc cgctattttt gtggtagagg acggagatca ttcttttaag atccggaaga
     1981 atccggctcg aacgcaggaa cacgtttttg ctgacgtgcg agagcaaatc cttttatttc
     2041 tgaagaaatt caaatgaatg cacgtctttg tcgctcaggt ctgaaggttg ggattgcact
     2101 cgtgatcatt ttcatttgtt ttgcagccac tcctcagccg gggagagaag aagcggcaga
     2161 atcgcagccg ctggctacgc aacttaaagt caaaaaggtg gagtggaatc tggatacatt
     2221 ctgcgctcat gtgtggattg aaaaccaatc gaaatccccg gttgacaggc tcatcattga
     2281 aataggggcg gattcgctcc gccctgtgcg aactacctgg aatcgcagcg gctctgaggc
     2341 agttcttgct ccgcatgcag agagatcttt tgaaatttgc gattcctctc tagcatccag
     2401 aaaggagcaa ctgacggcca gagtgatcat ggcgtcagct gtcatagatc ttgcgaagta
     2461 ttcggatctc tgcgattata tacgaagatg cgaggtgaac cctgaggcaa aaagactcct
     2521 ggccaatccc gaggcgtgcg agaagatgtt cgcgggtctg gagagaagtt ctcagacaat
     2581 ggcattcatt ccaatcatcg aggactgcgc gagaaagact gcatgctcgg agctagaaac
     2641 gttcaaatgc ttcgcattga gtgcgacccg atccatcccg acaaaatcag actcttccgt
     2701 ttcaccttga tcgccgcatg tctctgattg ttatcacatc gtactacaac ccagaacact
     2761 atcgttcgcg attcgaaaac tttgggcgct ttgtagcggg gcttgagtct gccggcattc
     2821 gttggatggt gggggaactt gcttttggag aagacgactt tgagctccct gatcacccct
     2881 cgatcgtgag aatgcgcgga ggggatgtga tgtggcagaa ggagagaatc ctgaatttgc
     2941 tattggatca attgccctcc gacgcaaagt acgtggcctg gatggattgc gatttgcttt
     3001 ttgagaatcc ggactgggca cgcgagaccg aacggaatct gaagtcggat gccgtagttc
     3061 agttgtttga ctacgtggtt cgtctcccca gagggcacgc gtcttatcgg ggagaaggag
     3121 agggatggga gggaatcggg tccattcaga gaagatcgcc taacgttctt ttgggagggg
     3181 attttgcagc gcacggacac acaggttttg cctgggccgc tcaccgctcg attcttgaga
     3241 aggtacgttt atacgatgcc tgcattgcag gcagcggaga tcacatgatg gctcatgctt
     3301 tctacggaga ctggcattct gcatgtgtgg atcgaatcct ttgcagaaat tcatttcatg
     3361 tatctcactt tgaaagatgg tgtcgcgctg tgtatcctct ggttcgcgcg aaggtaggat
     3421 tcaccagagg tgcgatctat catctatggc acggagaaac cacggatcga aggtatgtgc
     3481 ttcgcaatag agagctggcg gcctttggtt ttgatcctgt gcgtgacatt cgtgtcggcc
     3541 cggagggtct gtgggagtgg actggaaaaa aaagcggttt gcctgattgg gcccggtctt
     3601 actttaagca acggaaagag gacgcatgtt gaatcttctg aaatggccat ttcttttgtt
     3661 gtggcgcctc ctgactctgg gagcggggcg cgggcggatt ggagaggtgg cagatcgaat
     3721 tgcccgtcat ggcgatcgtt ttgcaaacgc ccgtcagaat gaaaaggcat ccatcgcaag
     3781 gcagtattct gggcgcgcgc gaaatgcatt cactgtgggc ggcgcagaat ccctggaaga
     3841 agattttctc gtggccgtag gcttgaaagc ggctcgccag gtcagagaag ccgtagaatc
     3901 agatgcaaat gatctgacgc gcgactcctg gcttcgcaat tcctcaagca actcgaggag
     3961 tagcgaatcc gcgtccagcg gatttgtatc ctcctcttcc agttcagatt cttcttcttc
     4021 ggaatcatcc gttgaagcag ggggagggag ttttggcggc ggcggggcga gacaaattcg
     4081 gcgacgcgct ggatttctct cgcacaatta acagtatagc ttatacgcca tttgatgcaa
     4141 aacgctgcgg taacgtgcat taaatatcct cgaaaagtga actacataat gaaac
//
LOCUS       DAORFF010000340         4180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12658_length_4180_cov_3.924606, whole genome shotgun sequence.
ACCESSION   DAORFF010000340 DAORFF010000000
VERSION     DAORFF010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12658_length_4180_cov_3.924606"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PKK76_13075"
     CDS             complement(<1..437)
                     /locus_tag="PKK76_13075"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNL69457.1"
                     /translation="MNCKHTIFLPVLFCFIYCQSPMVRGINEDNPALVQSGLDNGTNP
                     NVVIDGEPALHKALVRMAWNAARTLIDHGADLNAPDKMGNTPLMISMGNRNLELTEYM
                     MSRGADAKKKDTLSKTLLHYAAAVDRPDFISMLVKAGVDINAV"
     gene            1079..2182
                     /locus_tag="PKK76_13080"
     CDS             1079..2182
                     /locus_tag="PKK76_13080"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNL69458.1"
                     /translation="MAYIKKHERGLIFRKGDYVRSLGPGSHLPLWPGHELVILDTHKL
                     FKISKNLNELKADVELMREMKLLDVPDHSVALHFSDGRFQDVFGPGVFAYWNAAVEHT
                     FRIANLSAPVIGPEFDSALLSNPGLREFVQRFQIEAHQKGLLFFDGAYQGVLGPGAHF
                     FWKGPVQLAVTPIDMRQLQIDMTGQEILTMDRVSVRINFVCQYRIVDPEKIAQIKSYE
                     EQLYIILQLILREYVGTLTLDDLLAKKEEINQFVLARLREKAAVLGVEFASAGVKDIV
                     LPGDFKEIMNLVLIAEKKAQANLITRREEIASTRSLLNTARLMDENPTLYRLKELEFV
                     ERISDKVASISLSSGAGLLEQLTGMIATRKKEP"
     gene            2191..3750
                     /locus_tag="PKK76_13085"
     CDS             2191..3750
                     /locus_tag="PKK76_13085"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNL69459.1"
                     /translation="MAGMRTLFLLALVSAAFSLHAKPYIVSSVPNPRSQDGGFVSDPD
                     GILLAADRAELNGILRQIEQDTTAEYAVVILESIGTQIPKSFAVDLFNTWGIGKSGKD
                     NGLLLLVVLDQRRWEFETGYGLEGVLPDVVLSRLARDTFPDYFRKKQYGLAIIDVSRQ
                     IESILKHAAEEIGLSPEQRAEQLAREEEKLFAEYIQKTQTSRMWFGLGAFAFLCVYCA
                     VALFVRNNRKRPPSGQRGTAADKLILAPLSLWFWLPAVLIWPFMLVVVAYWNFYTIAF
                     IYQVSSFADSYLGYILGAVSYCMIGLMLIVQRQRRTTLLLKENPDPGESYRLIQEYLK
                     DAVPGLVFFPLFYGPYWIASKLRLGLLRRTPRKCLACNNVMRRLGESEEDPKLEPGQL
                     LEETLGSVDYDVWMCPQDSEIKIEKYILSSPYSECEACKFVTSVVIRNWTIQSPTYES
                     AGQGGEEQACRHCGRKKETRYVIAKLVRSGSASGGSYSSGGSSSRSSGSSSGGSFGGG
                     RSGGGGAGGSW"
BASE COUNT          927 a         1072 c         1166 g         1015 t
ORIGIN      
        1 tccacggcat tgatatccac acctgctttt accagcatgg aaatgaaatc gggacgatcc
       61 actgccgccg cataatgcag gagtgttttg ctcagtgtgt ctttcttctt agcgtccgct
      121 ccccgggaca tcatgtactc ggtgagctcc aggttccgat tgcccattga aatcatgagc
      181 ggtgtattgc ccatcttgtc gggtgcgttg agatcggcac catgatcaat gagggtgcgc
      241 gcagcattcc atgccattct gacaagtgct ttgtgcagtg caggctctcc gtctataaca
      301 acgttcggat tcgttccatt gtccaggccc gattgaacca gagcgggatt gtcctcattg
      361 attccccgca ccatgggaga ctgacaataa atgaaacaaa ataggacagg gagaaagatt
      421 gtgtgcttgc agttcatagg cccgattcaa acaggcatgt aaaatccatc aatcgcaaac
      481 ccaggctatt ccggggaaat acttagtata cctatactat cccaactcgt ggttcttgcg
      541 gaatgaggcc aaagacgaaa agaagaggcg ccaatggccg ttttctcgat tcgtaaacgc
      601 gaatagttcc gctttaaaat ccggacgtcc gcttgacaca gcggcgcctg acctttcact
      661 ccgaccgggc gacgttattc gatcgaagtc tgtgcaggga tgttcaagtc ccgcggtgcg
      721 ccagtctctg gctgcgcgta tgccgccttc tttcgaggct tcgttcacta tgggacctgt
      781 gtgtcgaagg ttcgagtcct tcaacgggaa accgtttagc tcagcggcag agcaacaggc
      841 tgttcaaagg gtgtggcgag ccccaaatcg cacaacggag aagagtccgg ctgcggcgaa
      901 agcgacggag catgcgccct gcgggccgtc gatgccgaaa gcgccagtgc gtttcctccg
      961 cgctcgagca gccagcgggc tgcaacaccg cgcaggttcc tcgcatcggc gagcggctca
     1021 agcgagcccc cgtgcagaat tcgtgcggcc gggttcttct ctgccttttt gaggttgtat
     1081 ggcttacata aagaagcatg aacgcggact tatttttcgc aaaggtgact acgttcgcag
     1141 ccttggtcct ggatcgcacc tacctctctg gccgggtcat gagcttgtca ttctagatac
     1201 acataaacta tttaagattt ctaagaatct caacgagctg aaggcagacg tggaactgat
     1261 gcgcgaaatg aaactgctcg atgtcccgga tcattcagtc gcgctgcatt tttccgacgg
     1321 gcgttttcag gatgttttcg gtcccggggt tttcgcatac tggaacgcgg ccgtcgaaca
     1381 cacttttcgg atcgcaaatc tctccgcgcc ggtcattggg ccggagtttg attcggcgct
     1441 tctgtcaaat cctggtttgc gggagtttgt gcagcggttc cagattgaag cccatcagaa
     1501 aggattgttg ttttttgatg gagcttatca gggcgtcctt ggtccgggtg cgcatttctt
     1561 ctggaagggg cccgtgcagt tggccgtcac accgatcgat atgcgccagc ttcagatcga
     1621 catgaccggt caggaaatct tgactatgga tcgcgtgagc gtgcgtatca actttgtatg
     1681 ccagtaccga atcgttgatc cagaaaagat tgcacagatc aagtcgtacg aggagcagct
     1741 ctacatcatt ctacagttga ttctccgaga gtatgtaggc acgctgacgc tcgatgacct
     1801 gcttgcgaag aaagaggaaa tcaatcagtt tgttctggca aggctccggg aaaaagcggc
     1861 tgttctcggt gttgaatttg cgagtgcggg tgtaaaggac attgttttgc cgggtgattt
     1921 caaagaaatc atgaatctgg ttctgattgc ggagaagaag gctcaggcga atctaattac
     1981 gcggcgcgag gaaattgcat ccacgcgcag tttactgaac actgccaggc tcatggacga
     2041 gaacccgaca ctgtatcggc tcaaggagct cgaattcgtt gagcgaatct cagacaaggt
     2101 cgccagtatc tccctatcgt cgggtgcggg tttgcttgag cagctaacgg gaatgattgc
     2161 gacgcgaaag aaagagccat gagagcaact atggccggca tgcgcacgct ctttctgctc
     2221 gccctcgttt ccgcagcttt ctcacttcac gccaaaccgt acatagtttc cagtgtgcca
     2281 aacccgagat ctcaggacgg cggatttgtc tctgatccag acggaattct tttggccgcc
     2341 gaccgcgcgg aactgaacgg aattctcaga cagattgagc aggacacaac ggcggaatac
     2401 gcggtcgtaa tccttgaatc catcggaacg caaattccga agtctttcgc cgtcgatctg
     2461 ttcaacacct ggggcattgg aaaaagcggc aaagacaacg gactattgct tcttgttgta
     2521 ctcgatcagc gccgctggga gtttgaaacg ggctacggtc tcgagggcgt gcttcccgac
     2581 gttgtgctat cgcgccttgc gcgagacacg tttcccgact actttcgtaa aaagcaatat
     2641 ggtcttgcga tcatcgacgt atcgaggcag atcgaaagca tcttgaagca tgccgcagaa
     2701 gagatcggtc tctcccctga acaacgcgca gagcagttgg ccagggaaga ggaaaagctt
     2761 ttcgcggaat acattcagaa aacacaaacc tcgcgcatgt ggtttggtct gggcgcattt
     2821 gcattcctgt gcgtatactg cgcagttgcg ttgtttgtgc gaaacaatcg gaagcgcccc
     2881 ccttccggtc agcgagggac agccgctgat aaactcattc tcgcaccgct ctctctgtgg
     2941 ttctggctcc ccgcagtcct tatctggcct ttcatgcttg tggttgtggc atactggaat
     3001 ttctacacca tcgcattcat ttatcaagtc tcaagcttcg ctgactcgta cctgggttac
     3061 atacttggcg ccgtatccta ctgcatgatc ggactcatgt tgattgtgca aaggcagcgg
     3121 agaactacgc ttctgctcaa agaaaaccca gatcccggtg aaagttaccg gctcattcag
     3181 gaatacttga aagacgcagt cccgggtctc gtcttctttc ctctcttcta tggaccctac
     3241 tggatcgctt caaagctgcg ccttggcttg ctgcgtcgca caccacgcaa gtgcctcgcg
     3301 tgcaataacg ttatgcggcg tctcggagag tcagaagaag atccgaagct tgaaccaggg
     3361 cagctgctgg aagagactct tggttcggtg gattacgatg tttggatgtg tccgcaagat
     3421 tcggaaatca agattgaaaa atacatcctg tcttcgcctt actctgagtg cgaagcctgc
     3481 aaattcgtca cctcggttgt cattcgcaac tggaccatcc aatcgccaac ttacgaaagt
     3541 gccggtcagg gcggtgaaga gcaagcctgt agacactgcg gccgcaagaa agaaacccgg
     3601 tatgtgatcg cgaaacttgt tcgctccggt tctgccagcg gaggatccta ctcgtcgggc
     3661 ggctcaagct ctcgaagctc gggcagttct tcggggggct cgtttggtgg tggacgatcc
     3721 ggaggcggag gagcaggcgg gagctggtga ctcttcccga gtacccgcgt ttgcggtgga
     3781 tgcggcaatg ctcttctggc gtgacagttc aaagccgtat ttcctaaatt tcattaaata
     3841 ttacacgaat tcccactgga gcgcagttgc ggtatggtcg tcacagcgag cacaggaggt
     3901 gcgagccttc acgactcggt ccatggaggg atcgcgtcgt gaagtggcga gcgtgccgca
     3961 atctgcggcg agggcggggt tgtctcgctt cgctcgacgt tcagagagga ggcacgcttc
     4021 gcgtgcatat gaggtgcgac ttgcgtcgca cacagaaaca aacccgcgtt tgcggtgaat
     4081 ggttgtggtg tggtgggagt tgagttgctc gtgagagttt cgcgggttct cacccgccca
     4141 tgatattctt ttgtattatg aaaatttcgg agagggcggg
//
LOCUS       DAORFF010000341         4175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12684_length_4175_cov_3.457039, whole genome shotgun sequence.
ACCESSION   DAORFF010000341 DAORFF010000000
VERSION     DAORFF010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12684_length_4175_cov_3.457039"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PKK76_13090"
     CDS             complement(<1..392)
                     /locus_tag="PKK76_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNL69460.1"
                     /translation="MQKLVVRCFSISVDGFGAGPEQSLENPLGIGGRDVHSWFFPTRT
                     FQQMFGESKGETGVDYDFAARGFENIGANILGRNMFGPIRGPWPNDEWKGWWGPNPPY
                     HSPVFVLTHHARQSIPMEGGNTFHFVTDG"
     gene            complement(395..967)
                     /locus_tag="PKK76_13095"
     CDS             complement(395..967)
                     /locus_tag="PKK76_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNL69461.1"
                     /translation="MKKNTKGQKASPAKSGDILATLQERFEKNMARHRGISWTSVQAR
                     LAKSPAKMRSLSAMEESGGEPDVIALDKKTGEVIFFDCSAETPSGRRSLCYDKEALAS
                     RKEHKPKDSAVNMANAMGIELLTEEEYRQLQELGEFDTKTSSWVKTPADVRKLGGAVF
                     CDRRYGRVFLYHNGAESYYAVRGFRGSLRI"
     gene            1076..2920
                     /gene="ilvD"
                     /locus_tag="PKK76_13100"
     CDS             1076..2920
                     /gene="ilvD"
                     /locus_tag="PKK76_13100"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNL69462.1"
                     /translation="MPVYRSRTTTAGRNMAGARALWRATGMKDSDFGKPIIAIANSFT
                     QFVPGHVHLKDLGQLVAREIEKAGAVAKEFNTIAVDDGIAMGHDGMLYSLPSRDIIAD
                     SVEYMVNAHCADAIVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKTRLAEHKL
                     DLVDAIVIGTDKNASDEKVEAYERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGS
                     MLATHADREELFLEAGRRIVEITKRYYEQDDSSVLPRSIANFQAFENAMSLDIAMGGS
                     TNTILHLLAAAQEGGVPFTMLDIDRLSRKVPQLCKVAPNTPKYHMEDVHRAGGVMGIL
                     AELNRGGLLHADLPTVHSKTFREALEKWDIAQSPSEAVSSFYRAGPAGIPTQVAFSQA
                     TRWPDLDRDRAEGCIRSVEHAYSREGGLAVLKGNIAPDGCVVKTSGVDESCWLFEGPA
                     YVVESQDQAVDDILNHKVKTGDVVVIRYEGPKGGPGMQEMLYPTSYLKSQGLGKACAL
                     LTDGRFSGGTSGLSIGHVSPEAASGGAIALIKNGDRIRIDIPNRSIDVLLSDSELEAR
                     RAAELARGSSAWKPAEVRPRKVSASLKAYAMLATSADRGAVRDLSKLD"
     gene            complement(2966..3319)
                     /locus_tag="PKK76_13105"
     CDS             complement(2966..3319)
                     /locus_tag="PKK76_13105"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNL69463.1"
                     /translation="MNPNVVGIGGIFFRAQDPKKLAAWYEEHLGVARSDSSQPWESSQ
                     GILVFAPFKKDSNYFPAEQALMVNFRVQKMDELLAELAAKGVRIDEKRQDESYGRFAW
                     IYDIEGNKIELWEPL"
     gene            complement(3373..>4175)
                     /locus_tag="PKK76_13110"
     CDS             complement(3373..>4175)
                     /locus_tag="PKK76_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNL69464.1"
                     /translation="FLATFAGAFYTAAVERYWIAVNEYNISFPRLPRQFDGFRIGFLS
                     DIHLGLFMPGAWVDAIVDRANALGVDALVLGGDYVHARKTDHELLLVWSKLKKLSAPS
                     GVFAVNGNHDHWANHDLPLRLLDSSGFSVRHKVATVKRGRATIAIAGVGDLWEDEFLL
                     DETLKDIPDSVFRIVLAHNPDSADLPHTSRVDLFLSGHTHGGQVRIPFLNWSPVIPVK
                     NKAYDLGLKQNTAEEKIFISKGLGWAILPIRFNVRPEIAIITLRSAEP"
BASE COUNT          978 a         1073 c         1093 g         1031 t
ORIGIN      
        1 ccatctgtta caaaatgaaa agtgttcccg ccttccatcg gaatggactg gcgcgcatgg
       61 tgagtgagaa caaagaccgg actgtgatac ggtggatttg gaccccacca gcctttccat
      121 tcgtcgttgg gccaggggcc gcgaatggga ccgaacatgt ttcggccgag tatgtttgcg
      181 ccaatgtttt caaatccgcg cgcggcgaaa tcgtaatcta ctcctgtttc ccctttgctt
      241 tcgccgaaca tttgttgaaa tgtcctggta ggaaaaaacc agctgtgtac atctctgcct
      301 ccaatcccaa gtggattttc caggctctgc tctggcccgg cgccaaagcc gtcgactgaa
      361 atggagaaac aacgaactac aagtttttgc atgattatat cctgagagat ccgcgaaagc
      421 ctcgcaccgc gtaataagac tctgctccgt tatgataaag gaaaactcta ccgtagcgac
      481 gatcgcaaaa gacggcccct cccagtttcc tcacatccgc cggcgttttc acccagctgg
      541 acgttttcgt gtcaaattcg ccgagctcct ggagttgtct gtattcctct tctgtgagta
      601 gctcgatgcc catcgcattt gccatgttca cggcgctgtc tttgggtttg tgttccttcc
      661 tggatgcgag cgcttctttg tcgtagcaaa gacttctgcg cccagaaggg gtttctgcag
      721 agcagtcaaa gaagattact tcgcctgtct tcttatcgag agcgattacg tctggttctc
      781 cacccgattc ttccatcgcg ctcagagatc gcattttagc cggagatttt gcgagtcttg
      841 cctggacgct tgtccacgaa attcccctgt ggcgagccat gttcttttca aagcgttctt
      901 gcagagtagc aagaatgtcc cctgatttag cgggcgaagc cttctggcct tttgtattct
      961 tcttcatatt cgatgacctg agttgtagtt tcaatctgcc tgtacggatc aagcgaaatg
     1021 ggatatttta ttgtccggca tggtgccgaa tgcaggtgtg gttctggaaa tcgtcatgcc
     1081 tgtttatcgc tctagaacca ccactgctgg ccgcaatatg gccggcgccc gcgcactgtg
     1141 gcgcgccacc ggcatgaagg actctgactt tggcaaaccc atcattgcca ttgcaaattc
     1201 ctttactcag tttgttcccg gtcacgtcca tttgaaagat ctgggtcaac ttgtggctcg
     1261 tgaaattgaa aaggcaggcg cggttgcaaa agaattcaat acgatcgcag tagatgacgg
     1321 aattgcgatg ggccatgatg gcatgctcta cagccttccc agcagggaca tcatagcgga
     1381 ttctgtggag tacatggtca acgcgcactg cgcggatgcg attgtctgca tttcgaattg
     1441 cgataagatc actcccggca tgctcatggc tgctatgcga ctcaacattc cagttgtgtt
     1501 tgtatccggc gggcccatgg aggccgggaa aaccagactc gcagagcata agctggatct
     1561 ggtggacgcc attgtaattg gcacagacaa aaacgcaagc gatgagaaag tagaagcata
     1621 cgaacgatct gcatgcccta cgtgtggttc gtgctctgga atgttcacag ccaattcgat
     1681 gaattgcctg acggaagcgc tgggcctgtc tctaccagga aacggatcga tgcttgccac
     1741 tcacgcagac cgcgaagagc tgtttctgga agccggtcgt cgcattgttg aaatcacaaa
     1801 aagatactat gaacaagatg actcaagcgt gcttcctcgc tcgattgcaa attttcaagc
     1861 atttgaaaat gcaatgtccc tggacattgc catgggtggc tccacaaata ccatcttaca
     1921 cctattagct gccgcacagg aaggcggagt gcctttcacc atgctcgaca tagatcgttt
     1981 gagtagaaaa gtgcctcagt tgtgcaaggt ggctcccaat actccaaagt atcatatgga
     2041 agacgttcat cgcgcaggcg gcgtgatggg aattctggca gaactcaacc gcggtggatt
     2101 gcttcacgca gatttgccta cagttcacag caaaacgttt cgagaagctc ttgaaaaatg
     2161 ggacatcgca caatcaccgt cggaggccgt ctctagcttc tatcgcgccg gtcccgctgg
     2221 gattccaacg caggttgcct ttagccaggc aactcgctgg ccagatctgg accgcgatcg
     2281 cgcggaaggt tgcattcgtt ctgtagagca tgcatacagc cgcgaaggtg gtctggcggt
     2341 gctcaaagga aacatagcgc cggatggctg tgtggttaag acttccggtg tggatgaaag
     2401 ctgctggttg tttgaagggc ctgcgtacgt ggtggaaagt caagaccagg ctgtagacga
     2461 tatactgaac cacaaagtca aaacaggcga cgtagtggtg attcgatacg aaggtccaaa
     2521 gggcgggcca ggaatgcagg aaatgcttta tccaaccagt tatctaaaat cgcagggact
     2581 gggaaaggcg tgcgccctgt tgacggatgg tcgattctct ggaggaacct ctggtctgag
     2641 cattggtcac gtatctccgg aagcagcttc cggtggagcg attgcactca tcaagaatgg
     2701 agaccgcatt cgcattgata ttcccaatcg ttcgattgat gttcttcttt ctgattccga
     2761 actggaagca agacgtgccg cggaattggc aagaggatct tctgcgtgga aaccggccga
     2821 ggttcgcccc aggaaagttt ccgcatctct caaggcatac gcgatgcttg ctacgagcgc
     2881 tgatcgcgga gcagtgagag acctgtccaa actcgactaa caacgcgaag gcgccagatt
     2941 ctatctgacg cgatgaaccg cgggatcaga gcggttccca gagttcgatt ttgtttcctt
     3001 cgatgtcgta gatccaggca aagcggccgt aggattcatc ctgacgtttc tcgtctatgc
     3061 gaaccccttt tgccgccagt tctgccagca gttcatccat tttctgcacg cgaaagttca
     3121 ccatgagtgc ttgttctgct ggaaagtagt tcgaatcctt tttgaacgga gcaaacacca
     3181 ggattccctg agaactctcc catggctggc tgctatcaga tcgcgccact ccaagatgct
     3241 cttcgtacca cgcagccagt ttctttggat cctgcgcgcg aaagaaaatt ccgccaatgc
     3301 ccactacgtt tggattcatg ggttcagaat ttggcggggc tttctgattc gcaacgaatt
     3361 ctttcgccta actcaaggct cagcacttct cagagtgatg attgctattt caggtctgac
     3421 attgaaccgg atcggcagaa tggcccaacc cagtcctttg gagatgaata tcttttcttc
     3481 cgcagtgttt tgcttcaagc ccagatcata ggccttgttt ttgactggaa tcacgggaga
     3541 ccagttgagg aagggaattc gaacctggcc tccatgcgtg tgtcctgata gaaacaaatc
     3601 gacgcgagat gtgtgcggta ggtccgcgga atccggattg tgcgcgagaa caattctgaa
     3661 cacagaatcg gggatgtctt tgagagtctc gtcgagcaga aactcgtctt cccacaaatc
     3721 tcctacacct gcaatcgcga tggtcgcgcg ccctcttttc actgtggcca ctttatgacg
     3781 cacggaaaag ccactcgaat cgagcaatcg caaggggaga tcgtgattgg cccagtgatc
     3841 gtgattgccg ttcaccgcaa acactccgga gggagcgctc agttttttca gtttggacca
     3901 cacgagcaat agctcatgat ccgtcttgcg cgcgtgaaca tagtcgccac ccaggaccag
     3961 tgcgtctacc cccagtgcgt tggcgcggtc tacgattgca tccacccagg cccctggcat
     4021 gaagagtccc aggtgaatgt ccgatagaaa gccaattcta aatccatcga actgtcgggg
     4081 caggcgggga aatgaaatgt tgtattcatt gaccgcgatc cagtagcgtt caacggcagc
     4141 agtgtaaaat gcaccagcaa aagttgccag aaatg
//
LOCUS       DAORFF010000342         4170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12707_length_4170_cov_3.782746, whole genome shotgun sequence.
ACCESSION   DAORFF010000342 DAORFF010000000
VERSION     DAORFF010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12707_length_4170_cov_3.782746"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="PKK76_13115"
     CDS             complement(<1..1295)
                     /locus_tag="PKK76_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69465.1"
                     /translation="MPVVLFLVASAFLGSIAFAIFLRRLDTPGLKINQVRRQGELQTE
                     KLDEIARNQIQAIRDATIEFELIVRQSRKLKDELRNDLEQYQNRIQSLRSDRDVLDTV
                     SVDLAQVAGSARTVASQVDRLDSGLQRLAYAQEEIAGIRKQLDDLSSISERKGQEAEI
                     RLGEIISRLVQDTETSTRLLTDQTRSNVSNVKDEFQDLTYKLEDQFKQVQVVSERISS
                     LNMRLDEKWSQDASRLDEKFAQQEKNYLDRISSIEAGLGAIRQTAVEALQSEVARIRR
                     DLDGFNLEGISRRDEILSESRRMAEMMNTQIADFNEKHLAAESRLQRISEQQKNVLKE
                     KLEALTKEWDALEETRLASIETRIRGIEEEFDRLRAEQAEHLATEAHQAIEQIRAESG
                     EATALLRDARIMEEENLVRVKQEVLQIREEFQDKTRKIDT"
     gene            1404..2153
                     /locus_tag="PKK76_13120"
     CDS             1404..2153
                     /locus_tag="PKK76_13120"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNL69466.1"
                     /translation="MQIVGGSVRTVPIYSAFAMIYDEVMSHVPFQNWARYLAAQAGRR
                     QRLLDLACGTGKVLQTLLQYTAADTIGIDSSEPMLSIARRRLKGQAEIRPGNLTRIPV
                     EDASADWAICTHDSINYLMTEGQLTRHFKEVFRVLEPGGIYSFDAVTEQNMITEFDGQ
                     VREEMIDGVLLRWSNRYERDRRVLVSHLVFQDGSSIQVEMHEQQYYDSETILSLASRA
                     GFETGPVQGDYEDRPLRARDAFMNFNCTKPL"
     gene            2150..2563
                     /locus_tag="PKK76_13125"
     CDS             2150..2563
                     /locus_tag="PKK76_13125"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNL69467.1"
                     /translation="MNIPEPVMKFLNYRDRSTEEVRRYLRRKNIVSEAEIPAVIKKLE
                     DLGFANDIRFTENRIRYRIGNLYGPFYIQRELAGLGISAETFRPLLAELEPEFVDAAR
                     QYAAKIKGGLDGPRLEKRLLGRGFSTNQVKLALKA"
     gene            complement(2510..2953)
                     /locus_tag="PKK76_13130"
     CDS             complement(2510..2953)
                     /locus_tag="PKK76_13130"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002732758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNL69468.1"
                     /translation="MTPGFFQISLKLFLMRGDELLILRDRASKYGDLPGGRLGETEIY
                     LPFPESLGREIREELGESVRYSLNPEPLFVFPHKMIAGHEALGIAFIGRYEGGEIELS
                     EEHDQMKWENVRSFDPRSVFFAHLLDAVLRFQSEFDLIRRKAPAE"
     gene            complement(2968..>4170)
                     /locus_tag="PKK76_13135"
     CDS             complement(2968..>4170)
                     /locus_tag="PKK76_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69469.1"
                     /translation="GKRWEGFPLNYGFLSNYNRYREEGSREAGEQLAGHLHRLLSAWT
                     ETGSPDQDFLEQFQEFMEELQTLPPDQNDLLDLGLMHAVEAFNLLKENRPEEALRIAS
                     DARFPFLAYLDENSYRSLTNVTFHEIDFQIYERIRGNFPEESARNYLLNAKRTDLWAA
                     IRYLEGLENREEAIEILSGMIERRIVLPERLILLAFWLLLDPDILTSERRPFFEQEQL
                     LESLARVASPGMATEGLDTGWVERMNPLYENEILFFYQAYFEISGSPLSPGWIHLLEK
                     SISNHWRVTIPEFPDEVHEPNPEFAGSILHLIEDEYIENLLKTSLILPLFFENLEKYV
                     DFSFYEISSGIARKEAIFMEELGHFLGRLTSEISELLRNRLEECAEILDCDLTHSDRG
                     FILQRRHI"
BASE COUNT          987 a         1052 c         1031 g         1100 t
ORIGIN      
        1 gtgtcgatct tgcgcgtttt gtcctggaac tcttcacgaa tttgaaggac ttcctgtttc
       61 actcgaacca ggttttcctc ttccataatg cgtgcgtcgc gaagaagggc cgtcgcttct
      121 ccagactcag cacgaatctg ctcaattgct tgatgagctt ccgtggccag gtgttctgcc
      181 tgttctgcgc gcaggcgatc gaactcttct tcgattccac gaattctggt ttctatggat
      241 gccagtcttg tttcttccag tgcgtcccat tctttcgtaa gcgcttccag tttctctttg
      301 agaacgttct tttgctgttc tgaaatgcgt tgcagtctgc tttctgcggc cagatgcttc
      361 tcattgaagt ccgcaatttg cgtgttcatc atctctgcca tgcgacggct ttcacttaga
      421 atttcatctc tgcgcgagat accttctaga ttgaatccat cgaggtcacg gcggatgcgc
      481 gccacttcgc tttgaagagc ttctaccgct gtttgccgga tcgcgccaag tcctgcttca
      541 atagaagaaa ttcgatcgag gtagttcttt tcttgctgag caaatttctc atcgaggcgc
      601 gaagcatcct gagaccattt ttcgtccagg cgcatgttga gacttgagat tcgttcagat
      661 actacctgca cctgcttgaa ttgatcttcc agtttgtaag tgagatcctg gaattcatct
      721 ttcacattgg atacattgga tctggtctgg tctgtaagaa gtcgagtgga tgtttctgta
      781 tcctgcacca gacgcgaaat gatttcgccc aggcgaatct ccgcttcctg tcccttgcgt
      841 tctgatatag aggaaagatc gtcgagctgc ttgcgaattc ccgcgatttc ttcctgagca
      901 taggcgagtc tctgcaggcc ggagtccagt ctgtccacct gcgaagcaac tgtgcgagcg
      961 ctgcccgcca cctgcgccag gtccacggac acagtgtcca gaacgtcgcg atctgagcgc
     1021 agactctgaa tgcgattctg gtattgctcc aggtcgtttc tgagttcatc tttcagttta
     1081 cgggattgac gcacgatgag ttcaaattct atcgtcgcat cacggatcgc ctggatctga
     1141 tttctggcaa tttcgtccag cttttcggtc tgcagttcac cctgacggcg aacttgattg
     1201 atcttcaggc caggtgtatc gagccgtctg agaaagatgg caaatgcaat tgagcccaga
     1261 aaagcgcttg caaccagaaa tagtacaaca ggcatttcca ccataattca gccccgaaag
     1321 cgtaatgcga cccgcatgat attatgtcgc atatgcgagc agggcaggaa tccacgcaca
     1381 tggcaagaaa aaaaggagaa tccgtgcaaa tagttggagg ttctgtaaga acggttccca
     1441 tctattctgc gtttgccatg atctacgatg aggtcatgtc tcacgttcct ttccaaaact
     1501 gggccagata cctggccgct caggccggga ggcggcagcg gttgctcgat ctggcatgcg
     1561 gcacaggcaa agtgctgcaa actttgcttc agtacactgc cgccgataca atcgggattg
     1621 attcctccga gcccatgctt tccattgcgc ggcgtcgtct caaagggcag gcggagattc
     1681 ggcctggaaa cctcaccaga atcccggtag aagatgccag cgcagactgg gccatttgca
     1741 ctcacgacag catcaactat ctaatgacag aaggccagct caccaggcac tttaaagagg
     1801 tctttcgcgt cctcgaacca ggcggaatct attcttttga cgcagttaca gagcaaaaca
     1861 tgattacgga gttcgacggc caggtccgag aggaaatgat agacggtgta cttttgcgct
     1921 ggtccaatcg gtatgagcgt gaccggcgag tgcttgtttc gcatcttgtc tttcaggatg
     1981 gctcgagcat ccaggtggag atgcacgagc agcaatacta tgattccgaa actattttgt
     2041 ctcttgcgtc cagggcagga tttgaaacag gcccagtcca gggagactac gaagacagac
     2101 cactgcgcgc acgcgacgcc ttcatgaact tcaactgcac aaagccctta tgaatattcc
     2161 agaaccagtc atgaagtttc tgaactacag ggacagatct acagaagaag tgcgaagata
     2221 ccttcgccgc aagaatatcg taagcgaagc ggaaattccg gccgtgatca agaagctcga
     2281 agatttggga ttcgctaacg acatacgctt cacagagaac aggattcgat accgcatcgg
     2341 aaatctgtac ggccctttct atattcagag ggaactggca ggtcttggaa tttcggcaga
     2401 gacgtttcgt ccgcttcttg cggaacttga gccggagttt gtggatgctg cgcgtcagta
     2461 cgcagcgaaa atcaaggggg gcctggacgg gccaagactc gaaaagcggc tactcggccg
     2521 gggcttttct acgaatcagg tcaaactcgc tttgaaagcg taagaccgca tccagaagat
     2581 gtgcaaagaa aacggaacga ggatcaaagg atctcacatt ctcccatttc atctggtcat
     2641 gctcttctga tagttcgatt tctcctcctt catacctgcc aatgaacgcg atcccgagtg
     2701 cttcgtggcc tgcgatcatc ttgtgcggaa aaacgaatag tggttctgga ttgagcgaat
     2761 atcgaacgct ctctcccagc tcttctctga tttcgcggcc cagggattca ggaaaaggta
     2821 agtagatttc agtttctccg agcctgcctc ctggcaggtc gccatactta cttgctcgat
     2881 ctcgcaggat caagagctcg tcgccgcgca tcagaaatag ttttaagctg atttggaaaa
     2941 atccaggagt catacgttat tccgttctca aatgtggcgc cgctgcaaaa tgaaacctct
     3001 gtcagaatga gtcagatcgc agtccaggat ctcagcgcac tcctccagtc tatttcgcag
     3061 tagttctgat atttcagacg tcaggcgacc aaggaaatgc ccgagttctt ccatgaagat
     3121 ggcttctttc ctggcgatcc cggatgagat ttcataaaat gaaaaatcta catacttctc
     3181 caggttctcg aagaaaagag gcaaaatcag gctggttttc agaaggtttt ctatgtattc
     3241 atcttcaatc aggtgcagga tgctgcccgc aaattctgga tttggctcgt gcacttcatc
     3301 tggaaactct ggaatggtga ccctccaatg attcgaaatg cttttctcca gaaggtgaat
     3361 ccatccaggg ctcagcgggc tgccgctgat ctcaaaatac gcctgataaa agaatagaat
     3421 ctcattttca tagagtggat tcattctttc cacccagcct gtatcgaggc cttctgttgc
     3481 cattccagga gaagcaacac gcgccaggga ttcaagcagt tgctcctgtt caaaaaatgg
     3541 cctgcgttct gaagtcagga tgtctggatc gagtaacagc cagaaggcga gaaggatcag
     3601 ccgttccggt agaacgatgc gtctttctat catcccggag agtatttcaa ttgcttcttc
     3661 tctgttctcc aatccttcca ggtatcgaat ggcagcccac agatctgttc gtttggcgtt
     3721 cagcaaatag ttgcgcgcgg attcttccgg aaagtttccg cgaattctct cgtagatttg
     3781 aaaatcaatt tcgtgaaagg ttacgttggt gagactgcga taggagtttt catccagata
     3841 ggcaagaaat ggaaatcgag catccgatgc aatgcgcaac gcttcttctg gacgattctc
     3901 tttgagcaga ttgaatgctt ccacagcatg catcaaacct agatcgagca gatcgttttg
     3961 atcgggagga agagtctgca actcttccat gaattcctga aactgttcca ggaaatcctg
     4021 gtccgggctg ccagtctctg tccaggcact cagaagtctg tgaaggtggc ccgcgagttg
     4081 ttcgcctgct tcacggctgc cttcctctcg atagcgattg tagtttgata agaatccgta
     4141 gttcaagggg aagccttccc atctttttcc
//
LOCUS       DAORFF010000343         4168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12718_length_4168_cov_4.035740, whole genome shotgun sequence.
ACCESSION   DAORFF010000343 DAORFF010000000
VERSION     DAORFF010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12718_length_4168_cov_4.035740"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PKK76_13140"
     CDS             complement(<1..383)
                     /locus_tag="PKK76_13140"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNL69470.1"
                     /translation="MRRYSLLLALSAIGPLAGLQAVPSFEEVRSSYKSSEAVYLDRRG
                     RILQSIRLDESGRRLAWTSLDRISPALLSAVVRSEDKRFYSHSGADYLAILKAAFGRI
                     AKDTRRGASTITMQLASRLDDSLTPS"
     gene            complement(380..1072)
                     /locus_tag="PKK76_13145"
     CDS             complement(380..1072)
                     /locus_tag="PKK76_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNL69471.1"
                     /translation="MADNIISVESLVKSFGDPPVQILKSLTFDIPRRQFVAITGRSGS
                     GKSTLLYTVSGLDDVTSGRVQMLGSDMHSAKPGDIEKMRNTQIGFVFQFHYLLPELTG
                     MENVLMPARKVRKDKEYKPWAIELMKRFDLGGCEDKLPSQMSGGQQQRVALARALIMK
                     PAVLFADEPTGNLDSVNAESVLEIFKEANENFGTTIIMVTHDEDYAARAHRRIHLVDG
                     KIDKDTLRRGKA"
     gene            complement(1086..2327)
                     /locus_tag="PKK76_13150"
     CDS             complement(1086..2327)
                     /locus_tag="PKK76_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNL69472.1"
                     /translation="MLFLALKQLRARPRQTILTFIAIILGSTGYVVFSGLMLGFQDRI
                     LDSLINNDAHIKISARDQVIDRETFREVFFTDGGLKWAVPPSGKTDSTKLNGAQIWYQ
                     RLSQDPRVEAFEPQLSRQVIYVRGRFTVPGNLNGVVPTKHLKLTNIEKSIVNGSLMDL
                     NRGGAMVILGQELLNRLGARVGETIHVVASDGTKHPVKIVGIVRLGSRMIEATLGFAS
                     LETVQNVSGSAGEVSVVGVRLKDVSLAESVAQEWANYTNDKVESWQKANESILSVFFT
                     QDLVRNITTLTIILVVSFGIYNILNMVVNQKKKEIAILRSIGYSRSDVVVLFLYQGLL
                     LGFTGAVLGLVIGYLICQYLSTITLPGNQPIGFNKLLISFKYQIYIRAFLISCGASVL
                     ASYFPARAAGRLAPIEVIRQS"
     gene            complement(2329..3108)
                     /locus_tag="PKK76_13155"
     CDS             complement(2329..3108)
                     /locus_tag="PKK76_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNL69473.1"
                     /translation="MRELLIKVNRKWLALLLLPVAVVVVYFLFFRNGATVKPKRGPVV
                     VAVYSLGTVTADNVFSFKAGLTASIQDIYVREGDAVGRGQKLLLLDSGAVVSAPFGGT
                     ITKVAFQKGEIVMPGVPVLTLIDLKNRHILVSLDQESALRVKPGQKVQISFETMRGQK
                     FSGTVERLYPQNGQFIARIKSELPADALPEMTADVAIETERKENSILIPAETIVESHV
                     MRVRNHVRQKVKVETGAADGEWIEITNNVIEDNDEILIQKK"
     gene            complement(3228..3665)
                     /locus_tag="PKK76_13160"
     CDS             complement(3228..3665)
                     /locus_tag="PKK76_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNL69474.1"
                     /translation="MRSWTSEASFIAGQRTDVERIREIMNEPYRIILVTQVRVGIAGC
                     VSVDVSERLGHFGMLSVGVPFQKGGMAKALIRAAESWAADQGAREMRLQVLMNRPELI
                     SYYERQGYRMTEERIPFPNGEPRFGEPLIDGLMFGTMSRPISD"
     gene            complement(3761..>4168)
                     /locus_tag="PKK76_13165"
     CDS             complement(3761..>4168)
                     /locus_tag="PKK76_13165"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNL69475.1"
                     /translation="GVGGGGDDNPIADINITPFVDIVLVILIIFMITAPMLYQSTIDV
                     NLPKADSGQQSEKSTLTFTINKEGELKWDKEKVKWEDVPGKLKALGGSLSTKTAIITA
                     DTATSHGTVVKLMDILRKSGVTKVGITVEGQGR"
BASE COUNT         1052 a         1106 c          966 g         1044 t
ORIGIN      
        1 gaaggggtca gagaatcgtc gaggcgtgaa gcgagctgca tggtgattgt gctcgctccg
       61 cgtcttgtgt cttttgcgat tcttccaaac gcggctttca aaatggcaag atagtccgcg
      121 ccggaatgcg aatagaaccg cttgtcttcg ctgcgcacaa ctgcgctgag taaggctgga
      181 gatatccgat cgagagaagt ccaggcaagc ctgcgacccg actcatccag gcgaatgctc
      241 tgcagaattc tacctctcct gtccaggtac acggcttcgg aagatttgta ggaggatcga
      301 acttcttcaa acgaaggaac ggcctgcaat ccggcaagag ggccaatcgc agaaagtgca
      361 agcaagagcg aataacgtct catgcttttc cacggcgaag agtatcctta tcaattttgc
      421 cgtccaccag atgaattctt ctgtgcgccc gcgcggcata gtcttcatcg tgagtgacca
      481 tgatgatggt tgttccgaag ttctcgtttg cttctttaaa gatttcaagc acactttccg
      541 cgttcactga atcgagattt ccagtgggct catctgcgaa aagaacggca ggcttcatga
      601 tcaaagcccg cgcaagagcg acgcgctgtt gctggccacc ggacatctgg gacggaagtt
      661 tatcttcgca gcccccgaga tcaaaacgtt tcatgagttc aatagcccac ggtttatact
      721 ctttgtcttt gcgaaccttg cgcgcaggca taagcacatt ctccataccc gtcaattccg
      781 gcagcagata gtgaaactgg aatacaaagc caatctgcgt gttgcgcatc ttttcaatgt
      841 cacctggttt tgcactgtgc atatcggagc cgagcatttg cacgcgacca gacgtaacat
      901 cgtcaaggcc actcacagta taaagtaaag tagacttccc ggaaccggac cggccggtga
      961 tggcaacgaa ctgacgtctt ggaatatcaa acgtaagcga ttttagaatc tgcacgggcg
     1021 ggtcaccgaa tgatttaacc agagactcga cggatatgat gttgtctgcc atcaccattc
     1081 ccccgtcacg attgacgaat aacctcgatc ggtgccagac gccctgcagc ccttgccgga
     1141 aaatagctgg cgagcacgct ggcgccacag gagatcaaga acgctctgat gtagatctga
     1201 tacttaaagg atattagtaa tttattaaaa ccgattggct gatttcccgg aagtgtaatc
     1261 gtagatagat actggcaaat caaatagccg atgacgagtc ccaggaccgc tcctgtgaat
     1321 cccagcaaaa gtccctgata caagaacaac accacaacat ccgagcgaga gtaaccgatg
     1381 cttcgcagaa tcgcgatctc ctttttcttc tgattcacga ccatgttgag aatgttgtaa
     1441 attccgaaag atacgaccag gatgatcgtc agagtggtga tgtttcgcac gagatcttgt
     1501 gtaaaaaaaa cagaaagaat cgactcattt gccttttgcc agctttcgac tttgtcgttt
     1561 gtatagttgg cccattcctg cgcgacggat tctgcaagac ttacatcttt caatcgcact
     1621 ccaaccacgg aaacttctcc agcagatcct gagacattct gtacagtctc aagagatgca
     1681 aaacccagag tagcttcaat cattctggac cccagacgaa caattccaac aatcttcacc
     1741 gggtgtttgg ttccatcaga tgcaaccacg tggatcgttt cacccactcg cgcgcccaga
     1801 cgattcaaaa gttcctggcc aagaatcacc atggctccgc cgcgatttag atccatcaga
     1861 gatccgttca cgatagactt ctcaatattc gtgagcttca agtgcttggt gggaaccacg
     1921 ccatttagat ttcctggaac agtgaatcga cctctcacat aaatgacctg acgcgaaagc
     1981 tggggttcaa acgcttccac tctagggtcc tggctcaatc tctggtacca gatctgagct
     2041 ccgttcaact tcgtgctgtc tgtcttgccg gacggcggga ccgcccattt tagaccaccg
     2101 tctgtaaaga aaacttcgcg aaaggtctcg cgatcgatca cctgatcacg cgctgaaatc
     2161 tttatgtgag catcgttgtt gatgagactg tctagaatgc gatcctgaaa tccaagcata
     2221 agcccggaaa acactacata gcctgttgat cctagaatga tggcgatgaa agtaagaatg
     2281 gtctggcgcg gccgagccct gagttgtttg agtgcgagaa agagcatttc atttcttctg
     2341 gatcagaatt tcatcgttgt cttcgatcac gttattcgta atctcgatcc actctccgtc
     2401 tgccgctccg gtttctactt tcactttttg tctgacatga ttgcgaacgc gcataacgtg
     2461 agattccacg atcgtttcag ccggaattag aattgaattc tcttttctct ctgtttcaat
     2521 cgccacgtct gcagtcattt caggaagcgc atccgctggg agttccgact tgatgcgagc
     2581 aatgaactgc ccattctgcg gatagagcct ctcgacagtg cctgagaact tttgacctct
     2641 catggtctca aagctgattt gaactttctg ccccggcttc acgcgaagcg cagattcctg
     2701 atccagagaa accaggatat ggcgattctt caaatctatg agagtcaaaa ccggaacgcc
     2761 aggcatcacg atttcgcctt tctgaaaagc aactttcgtg atggttccgc caaacggagc
     2821 ggagacgact gcgccagaat caagcaagag caacttctga ccgcgaccga ctgcatcgcc
     2881 ttcgcgaacg taaatgtcct gaatgctcgc agtcaatcct gccttaaaag aaaacacatt
     2941 gtctgccgtc accgttccca gagaataaac cgccacaacg acagggcctc tctttggttt
     3001 cacggtagcg ccattgcgga agaacaagaa gtagacgacc acgacggcga cgggcagaag
     3061 aagaagcgcc agccatttcc gattcacttt gatcagcaat tcacgcattt cgttcctctt
     3121 ttcaataccc gcgagcctgc ctgcagctgt caagatgact caactcgatt ctggcgatga
     3181 atgatatgat ttttgttaga cccggaaatc cagattctgt tccaaaatca atcagagata
     3241 ggacgactca tcgtgccgaa cataaggccg tcaatgagag gttccccgaa acgcggttct
     3301 ccatttggaa atggaattct ttcctccgtc atgcggtaac cctgcctttc gtagtacgaa
     3361 atcaattcgg ggcgattcat caatacctgc agtctcatct cacgggcacc ctgatcggca
     3421 gcccaggatt ctgccgcgcg aatcagcgct ttggccattc cacctttctg aaaaggaact
     3481 ccaacactga gcataccaaa atgaccaagt ctttcgctga catccacgga aacgcaacca
     3541 gcaatgccaa cgcgcacctg agtaaccaga atgatcctgt aaggttcatt catgatttcg
     3601 cgaattcgct ctacgtctgt tcgctgccct gcaatgaagg acgcttcgct cgtccatgag
     3661 cgcaaaccgg tggttccgcg atagactgaa tttacaagat cagagatgcg ttgcgcttct
     3721 actgcgcggg cagggcgaat gctccagtct gccagatctt ttacctgccc tgaccttcta
     3781 cggtgatgcc tactttcgta acgcccgatt tgcgcagtat atccatgagt ttaacaacag
     3841 ttccgtgaga agtcgcagtg tccgctgtga tgatcgcggt cttcgtgctc aaagagcctc
     3901 cgagcgcttt gagtttaccc ggtacgtctt cccacttaac tttttcttta tcccacttga
     3961 gttcaccttc cttgttgata gtgaacgtga gagtagactt ttcactctgc tgtccgctat
     4021 ctgctttggg aaggtttacg tcgatcgtgc tttgatacag catgggcgct gtgatcatga
     4081 agataatcag aataactaga acgatatcca cgaaaggcgt aatgttaata tccgctatcg
     4141 gattgtcatc gcctccgccg ccaactcc
//
LOCUS       DAORFF010000344         4166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12731_length_4166_cov_4.315495, whole genome shotgun sequence.
ACCESSION   DAORFF010000344 DAORFF010000000
VERSION     DAORFF010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12731_length_4166_cov_4.315495"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="PKK76_13170"
     CDS             <1..1139
                     /locus_tag="PKK76_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HNL69476.1"
                     /translation="GCNFCGGCMVGCRFDAKNTLDKNYLYFAEKLGVKVFPETKAVDI
                     IPLSADGSAGYEIRTKRTTGFFGLPNRTFRAKGIVFSAGVLGTMKLLLTMQETGRLTR
                     LSGRVGHMVRTNSEAIVGATSLSSKVDYSKGIAITSSVHPEEHTHIEPVRYSRGSDAM
                     GSLASVLTDGGGKIPRQIRFLGNVIRHPIQFLRTIHPIGFAKKSIILLVMQTLDNHIH
                     IVNKRRLIWPFSKSLSSEQEDAHDRKIPTYIPMANEFARRLAKRIGGIPRSSYNEVLL
                     NIPTTAHILGGACMGETPEDGVVDTQNRVFGYQNMLVCDGSMVPVNLGVNPSLTITAF
                     TERAMSFIPPKEKSVQAFKFEKKWGITSRLTENVKKGGFRLKFW"
     gene            1313..3982
                     /locus_tag="PKK76_13175"
     CDS             1313..3982
                     /locus_tag="PKK76_13175"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNL69477.1"
                     /translation="MSDWEFRAGVPTQPLDPQGAVWTKTHLPSYGKPVQGFDQYRGWL
                     LFRSRLPSELTAQNGDQLAVDAGLLSDVARVYVNDSLAGELGNVDPYSTGSMVTFVRT
                     VPNSIRLKSDGNYLYLALYTDGRYPIFASENPRVGAAEDILRDYYQSELLSIALLGVY
                     LLVGLYYLFLWSRRTKEVYHLFFGLLCVLLCFYWFFRLGSRDVVFGNHALLRSKVEYS
                     LLFLISPIFSFFLSQFFHGKYDRVGLLLTPFYALLLIVTAFGSYSVAVMCLTIWQFCI
                     MPIIAYYIFYIVREVYRRNPSALYLVPGLVALSLGAVHDILAIRGWISTPMIARFAFP
                     VFILGGALVLTHRFVFVHTQVEELLGNAYRLENFRTELLSIEPGASITELLERILVVL
                     ESLFECEKSFAIVPDAFGALHFTQELPEEIRNLILKGSRLHRRTKLISAEFFETYGSV
                     LQMELLKLEPSSPEGKQHLVSLRATELVFQKVAELGHKIAIALPHESEVLGMVFLGAK
                     KNKEKYSEAELSLIKTFQYSITQAIRNKMLFSEISQLKGEAEERVEKLSEYVIDRRNS
                     VQMEVKEKTIVYSSPSMVDVYEQAKKYADANKPVLISGETGTGKELIARAVHHFRNPD
                     APFIAVNCAAIPENLWESEVFGHARGAFTSAHGTHAGLIERAKGGTLFLDEIGEMPIS
                     VQPKMLRLLQERKFSRVGGEDELPASCQFLFATNRSLLDMQREGRFREDLYYRINVFQ
                     IHIPPLRDRRAEVPDLVRFFLAQYSRELRGRDTTVEEPALSALCRYSWPGNVRELENC
                     MIQALVHCKGEVLEVEDLPPVIRTFTQTRLAAEPPVQFKTAVSFDMLLADYSRKLILA
                     AMSRVKGNKLQTAKLLGLKRTTLYNKMKELGLR"
BASE COUNT         1041 a          974 c         1040 g         1111 t
ORIGIN      
        1 ctggctgcaa tttctgcggt ggatgcatgg tcggctgtag attcgacgcg aagaacactc
       61 tggataagaa ctatttatat ttcgcagaga agctgggcgt aaaagtattc ccggaaacaa
      121 aagcagtaga tatcattccg ctttctgcgg atggatctgc cggttacgaa attcgcacaa
      181 agcgcacgac tggattcttt ggactcccca atcgcacatt tcgcgcaaag ggaatcgtgt
      241 tctctgcagg cgttcttgga accatgaaac ttcttttgac catgcaggaa accggccgcc
      301 tcactcgcct ttcgggtaga gtgggccata tggttcgcac aaacagcgag gcgatcgtag
      361 gagcaacttc gctttcttcg aaagtggatt actcaaaggg aattgcgatc acgtcgagcg
      421 ttcatccgga agagcacact cacatcgaac ctgtgcgcta ttctcgcggt tcggatgcga
      481 tgggatctct tgcaagtgta ctgacagacg gaggtggaaa gattccacgc cagattcgtt
      541 ttcttggaaa cgtgattcgc catccaattc agtttctgcg aaccatccat ccaatcggat
      601 ttgcaaagaa gtccatcatt cttctggtca tgcagactct cgacaatcac attcatattg
      661 tgaacaaacg tcgtttgatc tggcctttca gcaagagcct ttcttctgaa caggaagacg
      721 cgcatgatcg taagattcct acatacattc caatggccaa tgagtttgcg cgtcgtctcg
      781 caaagagaat tggaggaatc ccccgcagtt cttacaatga agtacttttg aacattccaa
      841 ccactgctca tatactcggt ggtgcatgca tgggagaaac tccggaagac ggcgtggtag
      901 atacacagaa tcgtgtgttc ggttatcaga acatgcttgt atgcgatggc tctatggttc
      961 cagtgaatct tggtgtaaat ccaagtttga ccatcactgc gttcacagag cgcgcgatga
     1021 gtttcattcc gcctaaggaa aaatcggtgc aggcgttcaa gtttgagaag aagtggggaa
     1081 tcacatcgcg tctgacagaa aacgtgaaga agggcggatt tcgactcaag ttctggtaac
     1141 tctactgttg cacgcagtac aatttcgcct ttccagggtt tgcgctggac cagatgcgcg
     1201 actactgata tcgtagattg cgaatcacat gacccggcat agatgcgttt ggattttcgt
     1261 attgacctgt ctttttcaga cgggctgcgc acaggacgct aaaatcttta cattgagcga
     1321 ttgggaattt cgcgcaggcg ttcccactca acccctcgat ccacagggcg cagtctggac
     1381 gaagactcat cttccctctt acggtaagcc agttcagggc ttcgatcaat atcgcggatg
     1441 gcttttgttt cgttcgcgcc ttccatcaga actcacggcg caaaatgggg atcagcttgc
     1501 tgtggacgct ggattattgt ctgatgtggc gcgcgtatac gtaaacgatt cgctggcagg
     1561 cgagttgggc aatgtagatc cttacagcac cggatcgatg gtgacgttcg tcagaactgt
     1621 tccgaattcg attcgtttga aatcagatgg caactatctt taccttgctc tctacacgga
     1681 tggacgttat ccgatcttcg cttctgaaaa tccaagggtt ggcgctgcag aagatattct
     1741 gcgagactac taccagagcg aactgctgtc gattgctctt ctcggtgtct atttgcttgt
     1801 gggcctgtac tatctatttc tatggagcag aagaactaaa gaagtctacc atctgttctt
     1861 tggtctgctt tgtgttttgc tctgtttcta ctggtttttc aggctgggca gccgcgatgt
     1921 agtgtttgga aatcacgcat tgttgcgatc gaaggtagaa tatagtttac tttttttaat
     1981 atctcccata ttttctttct ttttgtccca gtttttccac ggcaaatacg acagggtcgg
     2041 cttgcttctg actccattct atgctcttct gctgatcgtc actgcatttg gatcctatag
     2101 tgttgcagtg atgtgcctca ccatatggca attctgcatc atgcctatca ttgcttacta
     2161 tatcttctat atagtgcgcg aggtttacag aagaaatcca agtgcattgt atctggttcc
     2221 tggtctcgtg gcgctgagtc tgggtgctgt gcatgacatc ctggcgattc gaggctggat
     2281 atccactcct atgatcgcgc gctttgcctt tccagttttc attctgggcg gggctctggt
     2341 cctcactcac agattcgttt ttgttcacac gcaggtcgaa gagcttcttg gaaatgcata
     2401 tcgccttgaa aattttcgca ctgagctttt gtctatagaa ccaggcgcta gcatcacaga
     2461 gctcctggaa agaattcttg ttgtccttga gagtttattc gaatgcgaga agtcattcgc
     2521 cattgttccg gatgcctttg gtgcccttca tttcacccag gaactgccag aagaaatcag
     2581 aaacctgatt ttgaaaggga gtcggttgca tcgccgcaca aaacttattt ccgctgagtt
     2641 tttcgagaca tacggatctg ttcttcaaat ggaactcctg aaactcgagc cttcttctcc
     2701 agaagggaag cagcatctgg tcagtctaag agccacagag cttgtgtttc agaaagtagc
     2761 agaactggga cacaagattg ccatagcact tccgcacgaa tcagaagtac tcgggatggt
     2821 gtttcttggc gcgaagaaaa acaaagaaaa gtattcggaa gcggaattgt ctctcatcaa
     2881 aacctttcaa tactctatca cgcaggccat tcgcaacaaa atgctttttt ctgagatctc
     2941 tcaactgaaa ggagaggcgg aagaacgagt ggagaaactt tccgaatacg tcatcgatcg
     3001 caggaactcg gttcagatgg aagtgaaaga gaaaaccatt gtctattcga gtccatccat
     3061 ggtcgacgta tacgaacagg caaagaagta cgcggacgca aacaagccag ttctgatttc
     3121 cggagaaaca ggaacgggca aagagctcat cgcacgtgct gttcatcact tcagaaaccc
     3181 ggatgctcct ttcattgcgg tgaactgcgc tgccattccc gagaatctgt gggagagtga
     3241 ggtgtttggg catgcgagag gtgcttttac gagcgcgcat ggaacccatg cgggtctcat
     3301 tgagagagca aaaggaggaa ctctctttct ggacgaaatt ggagaaatgc cgatttccgt
     3361 tcagccaaag atgctccgcc ttttacaaga gagaaaattt tccagagtag gtggagaaga
     3421 tgaactacct gcatcgtgtc agttcttgtt tgcaacgaac aggagccttc tggatatgca
     3481 gagagaaggg agatttcgcg aagaccttta ctacagaatc aatgtatttc aaattcacat
     3541 tcctcctctt cgcgatcgtc gtgcggaggt tccagatctg gtgcgattct tcctggctca
     3601 atattcgcgc gaattgagag gacgcgatac tacagtggaa gagccggctc tctccgctct
     3661 gtgcaggtat tcctggccag gcaacgtgcg agagcttgag aattgcatga ttcaggctct
     3721 ggttcattgc aagggagaag tgctggaggt ggaagatctg ccgcctgtca tcaggacatt
     3781 cacgcaaacg cgtcttgcgg cagaaccgcc ggttcagttc aagactgcag tatcctttga
     3841 catgttactc gccgactatt cgcgcaagtt gattctagca gccatgtcca gagtgaaagg
     3901 gaacaaactc cagactgcga agcttcttgg attgaaacga accacgctct acaacaaaat
     3961 gaaagagctt ggactgcggt aatcgcgaat cacgctgaga agattcgaaa caacacccac
     4021 acaggcaggc cggcgtagag tacaacgcgc gcaatcgtct caagggtgcc gcgcatttgg
     4081 attgcgtatg tagctcggac tgaaatcata aagattgtta aagtttccac aatgacgtat
     4141 aacgctgccg caaagagggc tacacc
//
LOCUS       DAORFF010000345         4161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12751_length_4161_cov_2.974915, whole genome shotgun sequence.
ACCESSION   DAORFF010000345 DAORFF010000000
VERSION     DAORFF010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12751_length_4161_cov_2.974915"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1636
                     /gene="rfbH"
                     /locus_tag="PKK76_13180"
     CDS             56..1636
                     /gene="rfbH"
                     /locus_tag="PKK76_13180"
                     /inference="COORDINATES: protein motif:HMM:NF011936.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HNL69478.1"
                     /translation="MQIIAGNVYEIKLHDSPQSAVALEDGTKDSMVDFAVLFHTTPAT
                     DHVTWHEEGPFQGSHLAAAEVYRLPASSAISKNATAPDSLLADFFRKRTALAVKGNYR
                     FGHKKVPFEEGRPISYAGRVYDEKEMLNLADSALDFWLTAGRFSAKFESSLARYVGAR
                     KLLIANSGSSANLLAFSALTSSRLGDKRLVPGDEVISVAAGFPSTITPIIQNGCVPVF
                     LDVSLPTYNVDVKYLEEAVSPRTRAVMLAHTLGNPFDLAAIKAFCAKHNLWLIEDNCD
                     ALGTEYFIDGEWKKTGSIGDMGTFSFYPPHHMTMGEGGAVCTSRVLLEKIILSFRDWG
                     RDCWCDPGKDNTCGIRFEHKLGQLPQGYDHKYIYSHFGYNLKATDMQAAVGCAQLEKL
                     PSFVEARIKNFNLLYEGLKDLEDVLILPQATANSKPSWFGFLLTVRKGARFTRNQIVA
                     HLESKKIQTRMLFAGNFLRHPSFDEMRATGKGYRVIGDLSVTDQIMNDTFWVGVYPGR
                     TQSEIDFMIREIRNFCRK"
     gene            1638..2192
                     /gene="rfbC"
                     /locus_tag="PKK76_13185"
     CDS             1638..2192
                     /gene="rfbC"
                     /locus_tag="PKK76_13185"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNL69479.1"
                     /translation="MFTLRETGLPGCFEVLPRQISDVRGRFIKTFHAPSYSQLGLETE
                     FVEEYYTTSHRGVLRGLHFQIPPADHAKLVYCVAGSVFDAVLDLRKDSPTYGRFAHLE
                     LSEEKRNLLYVPRGIAHGFTTISDECTMIYKVTSVHSPAEDAGIRWDSAGIPWPGSNF
                     IISERDQSFPGLSQYNSPFRMKQV"
     gene            2192..3109
                     /locus_tag="PKK76_13190"
     CDS             2192..3109
                     /locus_tag="PKK76_13190"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNL69480.1"
                     /translation="MMSSLQGRTILLTGAGGFIGRRLKERLQKAGADVHCLIRAGTQA
                     FSARDHLLSGEGAAAVSNLQKICEDTKPDIVIHLASLFLASHTPADIDSLIDSNIRLG
                     TRLLEAMEKAGIRRIINTGTFWQHFENRPYDPVNLYAATKQAFEDILTYYVNSNAFQA
                     ITLQLYDTYGPGDTRPKVLALLNKIARENSHLGMSPGEQFLDLVHVDDVCAAFETAAV
                     RMLKDQVQGHEVYAVSSSNPVQLREIVNIFNHAAGSKLDIGWGERPYRAREVMHPYNK
                     GIPVPGWEPRISLHEGIRDFIHSNQESKT"
     gene            3106..4065
                     /locus_tag="PKK76_13195"
     CDS             3106..4065
                     /locus_tag="PKK76_13195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNL69481.1"
                     /translation="MKKKLISIVVPVYFEEELIAEFYSRMHPVMNQLKSRFDTELIFI
                     NDGSKDRSLEILLELTKKDSRVRILDFSRNFGHQRAITAGIDYADGDAVVIIDSDLQD
                     PPEVIPEMIAKWEEGYDVVGGTRKARKGETAFKLFTARIFYRLLNSLSEIPLPLDTGD
                     FRLMDRKVVKVIRTMKEEGRYVRGLIVWAGFRQSSVLYERDARFAGETKYSLKRMLGL
                     ALDAITSFSEKPLMLAVQLGLFITAGSFLAMLYLLISRILRPESVVAGWTSLFVAILF
                     VGGVQILVVGILGVYIGRIYRESRDRPLYIVQETYGFKKRNSK"
BASE COUNT         1084 a         1066 c         1036 g          975 t
ORIGIN      
        1 ctctggaaca aatcagagaa tacgcaagca ttcgtgcagc cggttgagga ttgaaatgca
       61 aataatagct gggaatgtat acgaaatcaa actgcacgac tcgcctcaat cggcagtggc
      121 actcgaagat ggaacaaagg attccatggt ggatttcgct gtgcttttcc acactactcc
      181 agccacagat catgtgacct ggcacgaaga aggtcctttc cagggcagcc acctggcggc
      241 agccgaagtc tatcgcctgc cagcctcctc tgccatttca aaaaacgcaa ccgcacccga
      301 ttcgctcctc gcagattttt tccgcaaacg cactgctctt gcagtcaaag gcaattatcg
      361 gtttggtcac aagaaagtcc ccttcgaaga aggaagaccc atctcgtacg ccggacgagt
      421 ctatgatgaa aaggaaatgc tcaacctggc agattctgcc ctggacttct ggctgactgc
      481 aggacgattc agtgcgaaat tcgaatcaag cctggcgcga tacgtgggtg cgcgaaagct
      541 tctcatcgcg aattcaggat catccgcgaa cttactcgca ttttctgcgc taacttcgag
      601 caggctcggg gacaaaagac tggtacccgg agatgaagtc atcagtgttg ctgcaggctt
      661 tccatccaca attacgccga tcatccagaa cggatgtgtc cccgtttttc tcgatgtgtc
      721 tctccccaca tacaatgtag atgtaaaata tctggaagaa gcagtttctc cgcgcactcg
      781 agcagtcatg ctcgcacata ccctcggaaa tccatttgat ctggcggcaa tcaaagcgtt
      841 ctgcgcgaag cacaacctct ggctcatcga agacaactgt gacgctctgg gtacagagta
      901 cttcatagac ggagaatgga agaaaactgg atccatcgga gatatgggaa catttagttt
      961 ttatcctcca catcacatga ccatgggcga aggcggcgca gtgtgcactt cgcgcgtact
     1021 cctggaaaaa atcatactct cctttagaga ctggggtaga gattgctggt gcgatccagg
     1081 aaaagacaac acgtgcggaa ttcgattcga gcacaagctc ggtcagttac cacaaggata
     1141 tgatcacaaa tacatctact cccactttgg ctacaacctc aaggcaacgg acatgcaggc
     1201 tgcagtgggc tgcgcgcagc tcgaaaagct tccttctttt gtagaagcgc gcataaagaa
     1261 tttcaatcta ctctatgagg gtctcaaaga cctggaagat gtgttgattc taccgcaagc
     1321 aacggccaac tcaaagccaa gctggtttgg attcctgctc acggttcgca aaggtgcacg
     1381 ctttacgcgc aaccagattg tcgcgcatct ggaatcaaag aagatccaga ccagaatgct
     1441 gtttgctggc aatttccttc gccacccgag cttcgatgaa atgcgcgcca cgggcaaagg
     1501 ataccgcgtc atcggggacc tgagcgtcac agatcagatc atgaacgata cattctgggt
     1561 cggtgtttat ccagggcgta cgcagagtga gattgacttc atgatccgcg agattcgaaa
     1621 tttttgtcgg aaatgatatg tttactctgc gagagacagg tctacccggc tgcttcgaag
     1681 tactgccgcg gcagatttcg gatgtaagag gacggttcat caagacattc cacgcaccat
     1741 cctactcgca actgggattg gaaacagagt tcgtcgaaga atactatacg acctcgcatc
     1801 gcggtgttct tcgcggcctg cattttcaaa ttccgcccgc agatcatgcg aagctcgttt
     1861 attgcgtcgc tggatccgta tttgatgccg tgctcgacct tcgcaaagac tcacccacgt
     1921 atggcaggtt cgctcacctt gaactttcgg aagagaaacg caacctattg tatgttccgc
     1981 gcggaatcgc acacggcttt actacgattt ctgacgaatg cacgatgatc tacaaagtaa
     2041 cttcggttca ttcgcctgca gaggacgcag gcattcgctg ggatagcgct ggaatcccct
     2101 ggcctggcag caacttcatc atctcggaac gagaccaatc tttcccgggt ctatctcaat
     2161 acaatagccc attccggatg aaacaagtct gatgatgtca agcttgcaag gtaggaccat
     2221 tcttctcacc ggggcgggtg gattcattgg aaggcggttg aaagagcgcc tccaaaaagc
     2281 gggggcggat gtccattgtc tgattcgggc tggtactcag gctttctctg cccgagacca
     2341 tttgctgagc ggcgaaggtg cagccgcagt ctcgaacctg caaaagattt gcgaagacac
     2401 aaaaccggat attgtcatcc atctggcgtc tctctttctc gcctcacaca ctcctgcaga
     2461 catagactcc ctcatagaca gcaacattcg cctgggtacc cgacttctgg aagccatgga
     2521 gaaagcggga atccgtcgta tcatcaatac cggaaccttc tggcagcatt ttgaaaatcg
     2581 gccttacgat ccggtcaatc tgtacgcagc caccaaacag gcatttgaag acattcttac
     2641 gtactacgta aattcaaatg cgttccaggc aatcacgctt caactctatg atacttatgg
     2701 tccaggagac acgcgaccca aagtgcttgc tttgctgaat aagatcgcgc gcgaaaattc
     2761 acatctggga atgtcaccag gagagcagtt tctggacctc gtgcatgtag acgatgtgtg
     2821 cgccgcattt gaaacagccg cagttcgcat gctcaaagat caggtccagg ggcatgaggt
     2881 ctatgccgtc tcgtcgagca atccggtcca gctacgcgaa atagtaaata tctttaacca
     2941 tgccgctggc agcaaactgg acattggctg gggcgaaagg ccataccggg cgcgcgaagt
     3001 aatgcatcca tacaacaagg gcatacctgt gcccggttgg gagccccgca tctcacttca
     3061 cgaaggcatt cgggatttca tccattcaaa tcaggagagc aaaacatgaa aaagaaactc
     3121 atctccattg tagtccctgt gtactttgaa gaggaactga tcgctgaatt ctattccaga
     3181 atgcatccag tgatgaatca actgaagtcc agatttgaca ctgaactgat attcatcaat
     3241 gacgggagca aagacagatc tctggaaatc ttgcttgagc tgacgaaaaa agattcgcga
     3301 gtccggattc tggatttctc gcgcaatttt ggccaccagc gcgccatcac ggccggcatt
     3361 gattacgcgg acggggacgc agtggtcatc atcgatagcg acctgcagga tccaccggaa
     3421 gtcatcccgg agatgattgc gaaatgggaa gaaggctatg acgtggttgg cggcacgcgc
     3481 aaagctcgaa aaggggaaac agccttcaaa ctcttcactg cccgcatttt ctacagactc
     3541 ctcaacagtc tcagtgaaat acctctcccg ctggatacgg gtgactttcg tttaatggat
     3601 cgtaaggtgg tgaaagtcat tcggaccatg aaagaggaag ggagatatgt gcgtggcctg
     3661 atcgtatggg ccggatttcg tcagagcagt gtgctctacg aacgcgatgc acgatttgcc
     3721 ggagaaacca aatacagcct caagcggatg ctcggactcg cgctggatgc aattacaagt
     3781 ttttcagaga aacctttgat gctcgcagtg cagctgggtc ttttcatcac agctggctca
     3841 tttctggcta tgctctatct gcttatcagc agaattctca gacctgagtc tgtggtcgcg
     3901 ggctggactt cattgtttgt ggcgattctg ttcgtaggtg gagtgcaaat cctggtggtc
     3961 ggaatccttg gtgtttatat tggaagaatc tatcgcgaat cgcgcgacag gccactctac
     4021 attgttcaag aaacctacgg cttcaaaaaa cggaattcaa agtgaaatca ccggaaatct
     4081 ctgtctgcat tccggtgtat aacacggaac ctttcattgc agaggcaatc gaatcggccc
     4141 tgaatcaaac cttcaaagac t
//
LOCUS       DAORFF010000346         4160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12756_length_4160_cov_3.160292, whole genome shotgun sequence.
ACCESSION   DAORFF010000346 DAORFF010000000
VERSION     DAORFF010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12756_length_4160_cov_3.160292"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKK76_13200"
     CDS             complement(<1..398)
                     /locus_tag="PKK76_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69482.1"
                     /translation="MELKPRYKHTEVGVIPEEWELVKLRSLCPKSAYGPRFSSTLYSA
                     HGNVGTLRTTDMDDSGKISYETIPFAHLDENLFKAHFLEDGAFVVTRSGTCGIAAIFH
                     KIERPVVAGAFLIRFQFNDTVHRSFLKDML"
     gene            complement(389..2785)
                     /locus_tag="PKK76_13205"
     CDS             complement(389..2785)
                     /locus_tag="PKK76_13205"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HNL69483.1"
                     /translation="MPIKKSELYSSIWQSCDELRGGMDASQYKDYVLVLLFIKYISDK
                     YSGGAFEPIKIPKGASFKDMVALKGKSDIGDKINTQIIGPLGKANNLSDMPDFNSEKL
                     GSGKEKVERLGNLISIFQNPALDFSGNRAEGDDLLGDAYEYLMRHFATESGKSKGQFY
                     TPAEVSRVIAQVIGIGEAKTTASTTVYDPTCGSGSLLLKVADEARTKVTLYGQEKDVA
                     TSSLARMNMILHDNPEALIENGNTLADPKFKEKEALKAFDYVVANPPFSDKRWSTGVD
                     TEKDPFGRFETFGVPPGKQGDYAYLLHIVRSLKKNGKGACILPHGVLFRGNAEAEIRK
                     NLVKHGYVKGIIGLPANLFYGTGIPACIIVLDKEEAPARKGIFMIDASAGYLKDGNKN
                     RLRARDIHKIVDVFKNLQEVPKYARMVSLAEIEKNEFNLNLPRYIDSQEAEDLQDIEG
                     HLRGGIPARDVDALSSYWKICPGLRKTLFKENRPGYVDLSVEKSAIKSAIFDHPEFSA
                     FIQKMNSHFQTWKEKNTAHLKKLKSGFHPKELIASLSEELLSHYEGQPLIDPYSVYQH
                     LMDYWSETMQDDGYMISADGWTAQTQRVLVKDKSGKEKDKGWVCDLIPKELVVHRFFA
                     KERETISTMESEAEGLAAQIQELEEEQTGEDGAFSELEKVNRASVAARLKEIAGEADS
                     KEEIQALETWTKLNTQEAELRRKIKELETELDDLAYKKYPKLTESEVKTLVVEDKWMA
                     ALDAQVHGEMDRISQTLTQRVKELAERYTTTLPEITKTVADLEKKVSAHLAKMGFAWN
                     "
     gene            2882..3280
                     /locus_tag="PKK76_13210"
     CDS             2882..3280
                     /locus_tag="PKK76_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69484.1"
                     /translation="MAKPSSCFHFVPGHMAYEEDKEVDWSGLVAVGSLLGNLLQYTEK
                     NNLEKTLHWTGERLRAIAEDRERVIAMLHQVQKAYAVLKADADRMAATNKDLLTKLTE
                     SESEKGRLRNEVTELKEAIGILEKRLNDAK"
     gene            3267..3488
                     /locus_tag="PKK76_13215"
     CDS             3267..3488
                     /locus_tag="PKK76_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69485.1"
                     /translation="MMQSELARVQEQLLEDGRNWSQHALQFGQFLAASTTSLDMNVSD
                     RARYTLPPKVNLLKPVDIVKIIEDDRYNA"
BASE COUNT          986 a         1079 c          990 g         1105 t
ORIGIN      
        1 ttgagcatgt ctttaagaaa tgatctgtgc accgtgtcat tgaactggaa tcggatcaaa
       61 aaagcgccgg caacaacggg tcgctcaatc ttgtgaaaaa tggccgcgat gccgcaggtg
      121 ccactccgag tcacaacgaa tgcgccgtct tctaagaagt gcgctttgaa cagattttca
      181 tccaggtgcg caaaagggat tgtctcgtag gaaattttcc cagaatcatc catgtcagtt
      241 gtgcgcaacg tcccgacgtt gccgtgcgcg ctatacagcg tggaactaaa cctgggcccg
      301 tacgcggatt ttgggcaaag ggagcgcagc ttgactagct cccactcttc cggaatcaca
      361 ccgacttccg tgtgcttgta tcttggcttc agttccatgc aaaacccatc ttcgctagat
      421 gcgcgcttac ctttttctcc aggtccgcca cggtctttgt gatttcaggt agagtggtcg
      481 tatagcgttc cgccagttct ttcactctct gtgtgagagt ctgactgatt ctatccatct
      541 ccccgtggac ttgtgcatcc agagccgcca tccatttgtc ctccactacc agtgtcttga
      601 cttcagattc tgtgagtttg ggatatttct tgtacgcaag atcgtccagt tctgtttcta
      661 gttccttaat cttccggcgg agctccgctt cctgagtgtt gagctttgtc caggtttcaa
      721 gcgcctggat ttcttcttta gagtccgcct ctcccgcgat ctctttcaat cgagccgcga
      781 cggatgcgcg gttcactttc tccaattcag aaaaagcgcc gtcttctccg gtctgctcct
      841 cttccagttc ctgaatttgc gcggccaggc cttccgcttc actttccatt gtggagatgg
      901 tttcgcgctc ttttgcgaag aatcgatgaa ccacgagttc ttttggaatc aagtcacaga
      961 cccagccttt gtctttttct tttccgcttt tgtctttgac gagtactcgc tgcgtctgtg
     1021 ccgtccagcc gtctgcagag atcatgtaac catcgtcctg catggtctct gaccagtagt
     1081 ccatgagatg ctgatacaca gaatatggat cgatcagagg ctgaccctcg taatgcgaaa
     1141 gcagctcctc agaaagagag gcaatcagtt ctttaggatg aaagccggat ttaagtttct
     1201 tcagatgcgc tgtatttttc tctttccagg tctgaaaatg cgaattcatt ttttgaatga
     1261 acgcgctgaa ctcaggatga tcaaagatcg cagacttgat ggcagacttt tctacagata
     1321 ggtccacata gcccggtcga ttctctttaa agagagtttt ccggagtccg ggacaaatct
     1381 tccagtaaga ggaaagggca tccacatcgc gcgcaggaat tccgccgcgc agatgccctt
     1441 ctatgtcctg caaatcctcc gcttcctgcg aatcaatata tctgggcaaa ttcagattga
     1501 actcattctt ttcaatctcc gcgagagaaa ccatgcgtgc gtattttgga acttcctgca
     1561 ggttcttgaa gacatctacg attttgtgaa tgtctcgcgc gcgaagacga ttcttgtttc
     1621 catctttcag atagcctgca ctcgcatcga tcatgaagat ccctttgcga gcgggagctt
     1681 cttctttatc cagaacaata atgcatgcag gaattccagt gccgtaaaac aagttcgcag
     1741 gcagcccaat gatgcctttg acatagccgt gcttcaccag attcttgcgg atctccgctt
     1801 ccgcgtttcc gcgaaacaat actccatgag gcagaatgca cgcacccttc ccgttcttct
     1861 tgagagaacg cacaatgtga aggaggtagg cgtaatcgcc ctgctttcca gggggcactc
     1921 cgaatgtttc aaatctacca aacggatcct tctccgtatc cacgcccgtg ctccagcgct
     1981 tatcagagaa cggtggattt gcaactacat agtcaaaggc tttgagtgct tctttttctt
     2041 tgaattttgg atctgccagg gtgtttccgt tttcaatcaa cgcttccgga ttatcatgca
     2101 ggatcatatt catccgcgcg agactcgacg tggccacgtc tttttcctga ccgtataacg
     2161 ttacttttgt gcgggcttcg tctgccactt taagcaacaa cgacccggat ccgcaagttg
     2221 gatcatagac agtagtggat gcagttgttt tcgcttctcc tattccaatg acctgggcaa
     2281 tcacacgact cacttctgcg ggcgtgtaaa actgtccttt gctctttccg ctctccgttg
     2341 cgaagtgccg catgaggtac tcatacgcat cccccagcaa gtcatctccc tctgcgcggt
     2401 tcccggagaa atcaagagca ggattctgaa atatggaaat gagatttcct agcctttcta
     2461 ctttctcctt tccgcttcca agcttctctg agttgaagtc tggcatgtct gacagattgt
     2521 ttgcctttcc cagaggcccg atgatctgcg tattgatctt gtctccaatg tcgcttttgc
     2581 ccttgagagc gaccatgtcc ttgaagctgg cgcccttcgg gatcttgatt ggttcgaatg
     2641 ccccgccgct gtatttatcg ctgatgtatt tgatgaagag caataccaga acatagtctt
     2701 tgtactggct tgcatccatg ccgccgcgga gttcatcgca gctctgccag atggatgaat
     2761 acagttctga ttttttgatg ggcatttcca tctgaacgtg agtgagggca tccagcgtgc
     2821 aaggttttta gggaagaagg cgcacggaac ccggcgcgtg cttccgcagt gcaaaataac
     2881 gatggccaaa ccatcttcat gctttcattt tgtacctggg catatggcgt acgaagaaga
     2941 caaagaggtg gactggtcag gtctggtagc ggtcggaagc ctgctcggga acttgctcca
     3001 atacacagag aagaataacc tggaaaagac cctgcattgg accggggaac gcctccgcgc
     3061 catcgccgaa gatcgcgagc gtgtaattgc aatgctgcat caggttcaaa aagcttacgc
     3121 agtactgaaa gccgacgcag atcgaatggc cgcaaccaac aaggatttac tcacaaagtt
     3181 aacagagagc gaatcggaga aaggacgcct cagaaatgag gtcactgaac taaaggaagc
     3241 aatcggaatt cttgaaaaga gactcaatga tgcaaagtga actggcaagg gtgcaggaac
     3301 aacttttgga agatgggcga aactggagcc agcatgcatt gcagtttggc cagttccttg
     3361 ctgcaagcac tacgtcgctg gatatgaacg tatctgacag agcgagatat acgctgccgc
     3421 caaaagtgaa tttgctcaag cccgtcgata tcgtcaaaat catcgaggac gatcgctaca
     3481 acgcgtgatc cgtttctttg acacatctgc actggcactc gtggccttgc gccagaatgg
     3541 gtgggaactc gcagcgcaac tttatgaatc cgcgagcgaa gtgatagtcg cgtttcatac
     3601 gcgagtggaa ttctattcag cactaaatcg cttacaaacc gatcatgcga tcggtgtcac
     3661 agatgccgaa cagctctatg actggttcga cctagagagc cagcgaatgc gcgtgatagc
     3721 attttcagac cccgtcgaac aaagagctct ttccatctta aaacaacacc agatgaaaac
     3781 tctcgattgt cttcaactcg catgcgcgct ggaatcagga agcactttgg aaaggttcgt
     3841 gagctcagac aagagactcg ctcggattgc agaaaagttc gggttaagca gtggatgaat
     3901 cacccccgca acgtgctttc gaaaaatctc tgaagaatct tctgaacaga ccgctctcta
     3961 aaattcttcc gcccttcttg aaaaacaaga acatttggcg ccttgccttc agagagaccg
     4021 cggaaaatcg acgcagccca atcagtaata agataggtct ttgctttgca ccgcggggcc
     4081 gcaactttca gctcgcttcc attttccgaa agatcacgca cacgaaggtt caccgtctcc
     4141 tttgccgtta gttggagagc
//
LOCUS       DAORFF010000347         4158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12763_length_4158_cov_3.920058, whole genome shotgun sequence.
ACCESSION   DAORFF010000347 DAORFF010000000
VERSION     DAORFF010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12763_length_4158_cov_3.920058"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PKK76_13220"
     CDS             complement(<1..395)
                     /locus_tag="PKK76_13220"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNL69486.1"
                     /translation="MLKHTQIARFAVILLFFTACDALRTQHLRTEVIELGEYKIRAEI
                     ASTPRERERGLMYRTSIGEEEGMLFVFPKASPQAFWMKNTLIPLDVGYFDEQGFLIEY
                     FTMTPDDGVRTYPSSEPALYALEMKAGWYA"
     gene            595..1692
                     /locus_tag="PKK76_13225"
     CDS             595..1692
                     /locus_tag="PKK76_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69487.1"
                     /translation="MRINASLRENKDLNNRFRITGKSNRVGWWSRKKIHVYLLLATLS
                     CTPLLGDEQHTFEISAIQGAGTGSTTLSSDDLTRTLQANAAKAYLLQFTGYVLPINLG
                     DWNEINHSSGLDFRYTYANWLWLRVVYTETQDSRNTNALSLPTSTGLSPGIMERGYVS
                     EARQPEFAIGRRWLPTNNTTLDAGVSFGHGWTRTTVEDVSVFSNSFFGIPIQGLANET
                     RRESIESWGPLVCLSYSPATWAQISVEARYLFGHGSYSANRWDYRTGIAARLYAYENV
                     YGTRRERTQLVSLSTTIKYDMFFIRPRVTIGTLERTTTSASDLEVDPGAMGTPDAALR
                     ASLVNSGSFVSQKASVPLFNFDIALGAKIAL"
     gene            complement(2145..3482)
                     /locus_tag="PKK76_13230"
     CDS             complement(2145..3482)
                     /locus_tag="PKK76_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69488.1"
                     /translation="MARPIEESGGFMSKSMRISGLITLLAALSISLFVYCSSGSSKKD
                     SDDDKTVFGDCLDGKITVRGEAPIYTSMDAAEGKAKEDACRTAVEKCIGTQIASSSGV
                     GDGQSIGNEIFTKANGMCRNGKILERNEYPFDTFKMLRLTIRYEVSESELKSQIDQMQ
                     KLAGNPKVMILIREEYNIAGQGKRVESFTSRNAIAAAALRDYLVRKDYTVIDASSISL
                     GGMNEEVAAENPSQFPEDVKERAAKAGADVLIIGSLESNPQASIDLSDETMKLKMKSY
                     KATGNVTLMTLWGKGKVLGEFNTPMGGAQTTDLSAAQQSMKRYAVGSAPDPIKSPGGI
                     AKEINRRLSDQWSELTKNNVIQISISGLNQKSAGMFRDDLKGKTSVRDVNPLSESDGS
                     QEWEVTYPGRSFALADTISWNRDNPKIFQVIKESGKKPTVLSVTRGEIKIRFD"
     gene            3545..3916
                     /locus_tag="PKK76_13235"
     CDS             3545..3916
                     /locus_tag="PKK76_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69489.1"
                     /translation="MGEVKASLPSDVFLVQKALGIEMEKRAAILSADGRELQRKTRES
                     EEVLRQMGQMAASPKPLSREAIRLAEKLREESGENQRLLEKANHTIHRLLTEMGEKES
                     TYDPHARKQQSTGGVLLDASV"
     gene            3979..>4158
                     /locus_tag="PKK76_13240"
     CDS             3979..>4158
                     /locus_tag="PKK76_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69490.1"
                     /translation="MRIKLWGVRGSLPTPMGVQEYQTILGHALAHAQNDFRANPSITA
                     EQLYEGIPAQYRRVIG"
BASE COUNT         1072 a         1070 c          981 g         1035 t
ORIGIN      
        1 gcataccagc ctgccttcat ctcaagagcg tataacgctg gttcagaaga aggataagtg
       61 cgcactccat catccggtgt cattgtgaag tattctatca agaagccctg ctcgtcaaaa
      121 tagcctacat ccagcggaat gagagtattc ttcatccaga aagcctgagg agatgctttt
      181 ggaaaaacaa acagcatgcc ctcctcttct ccaatcgaag tgcgatacat caaaccacgt
      241 tccctctccc tgggagtgga agcaatctca gcccggattt tgtattcacc cagttcaatc
      301 acttccgtgc gcaaatgctg agtgcgcagc gcatcgcagg cagtgaaaaa gagtagaatg
      361 accgcgaagc gtgcaatctg tgtatgcttt agcattggtt tgaattgccc ggccccagtt
      421 gatcgtcgaa cgaaaaaagc tccaaagtat gattggacgt cagaaaccag atttgcagcg
      481 cgatacataa gcacggtcat ccttggtctt cagccagctg aatgcgtctg cactccaaca
      541 aacatccgta cagtttcccc gggttgggac ttgtccttga ccccgaagcg gtcaatgcga
      601 atcaatgcaa gcctacggga gaacaaagac ctgaacaatc gattccgcat aacgggtaag
      661 tccaacaggg taggatggtg gtcaagaaaa aagattcatg tctatctgct gctcgctaca
      721 ctgtcctgca ccccgttatt gggagatgaa cagcatactt ttgaaatctc agcaattcag
      781 ggagcgggaa ctggaagcac tacattatca tccgatgatt taactcgcac actccaggcc
      841 aatgctgcca aagcctactt gttacaattc actggctatg tgcttccaat taatttgggt
      901 gattggaatg agataaacca ttcgagcggt ttggatttcc ggtatactta cgcgaactgg
      961 ttatggttgc gagttgtgta caccgaaacc caggactcta gaaatacaaa cgccttgtcg
     1021 ctaccaacat caacggggct gtcccccgga atcatggaac gtgggtatgt gtcagaggcg
     1081 cggcaaccag aattcgcgat tggccgccgc tggctgccca caaataacac aacccttgat
     1141 gccggggttt ctttcgggca cggatggacg cgaacaacgg tggaagacgt ttccgtgttt
     1201 tcaaattcat tctttggcat tccaattcaa ggactggcca acgagacccg gcgcgagtct
     1261 atcgagtcct gggggccgct ggtttgcctg tcctattccc ccgccacctg ggcccaaatc
     1321 agcgttgaag cccgttacct gttcggacac ggaagttact cagccaatcg ttgggactat
     1381 cgaaccggta ttgccgcgcg tttgtatgca tacgagaatg tttatggaac acggcgggag
     1441 cgaacgcagc tagtcagcct atccacgacc ataaagtacg acatgttttt catccgccca
     1501 cgagtgacga tcggaaccct ggaacgtaca acgacgtccg cgtccgattt agaagtggat
     1561 ccgggagcaa tgggaactcc ggacgcagcc ttgcgcgcat ctttagtcaa ttcaggatca
     1621 tttgtcagtc aaaaagcgtc tgttccattg tttaacttcg acattgctct gggcgccaaa
     1681 atcgctttgt agactatgcg agtcattctg ttctactccc tttccttcgc atttgcgaca
     1741 tgccgcggtc cacagcttca acccaatact ttcaattcac cgcgcactga agacaacgtg
     1801 agcattcaaa ctgaacactc cactcctctg ccagatccgg tagaacgtca ttttgcgcaa
     1861 aaagaatgcc tggaaaatcg ccagacctgc aaatcaacct gtcaggcttt tctctggact
     1921 ggtttcttcg caaatgccgc ggtcaatcac acgataggag gtgagaacac gactgagact
     1981 tgcgaaacca gatgcaatcg cgaaaacctg tgcccgccaa tgatcaccac accgcagcgc
     2041 cgataaagag gattacacga gttcggaaag gcgcgctact taagctgaaa ccgtgtcaga
     2101 cgatcgacag ccggccaatg tttccaatgg ccggcatcaa agtttcaatc gaatcgaatt
     2161 ttgatctctc cgcgagtgac gctgagaacc gttggcttct tgcctgattc tttgattact
     2221 tgaaaaattt taggattgtc tcgattccag gaaatcgtgt ccgcgagagc aaaagaacga
     2281 ccgggataag tcacttccca ttcctggctt ccatcgcttt cactgagtgg attgacatca
     2341 cgaactgaag tcttgccctt cagatcatct ctaaacatac ccgcagactt ctgattcaat
     2401 cctgaaatgc tgatttgaat tacattgttt tttgtaagct cggaccactg atctgaaagc
     2461 ctgcgattga tttcttttgc aattccaccg ggtgatttga ttggatctgg agcagaacca
     2521 actgcatatc gcttcatgga ttgttgcgct gcggacaaat ctgttgtttg cgcaccaccc
     2581 atgggagtat tgaattctcc gagaactttt cctttgcccc atagagtcat aagagtcacg
     2641 ttgcctgttg ctttgtaaga cttcattttg agcttcatgg tttcatcgct gaggtcaata
     2701 gatgcctgag ggttggattc caggcttcca atgatgagaa catccgcccc cgctttcgct
     2761 gcgcgttctt ttacgtcttc tggaaactgc gaaggatttt ctgcggccac ttcttcgttc
     2821 attcctccaa gagaaataga agaagcatca atcacggtat agtctttgcg aaccaggtag
     2881 tcgcgaagtg ctgccgcggc gattgcattt ctagaagtga atgattctac tctctttccc
     2941 tgacctgcga tgttgtattc ttcgcgaatc aggatcatta cctttggatt gcctgcaagc
     3001 ttttgcatct gatcaatctg gcttttcagt tcagattcag agacctcgta gcggatggta
     3061 agacgaagca tcttgaatgt atcaaaagga tattcgtttc tttccaggat cttgccattg
     3121 cgacacatcc cgtttgcttt ggtgaagatt tcatttccga tggactgacc atctcccacg
     3181 ccggaggaac ttgcgatctg agtgccaatg cacttttcaa ctgcagtgcg gcatgcgtct
     3241 tcctttgcct taccttctgc cgcgtccatg ctggtataga taggagcttc accgcgcaca
     3301 gtaatcttgc catcgagaca atcgccgaat acagtcttat catcgtcaga atcttttttc
     3361 gaagaccctg aggaacagta tacgaacaat gaaatgctca aggccgccag gagcgttata
     3421 agaccggaaa ttcgcatgga tttagacata aagcctccgc tttcctcaat gggacgtgcc
     3481 aaaccgggct gcggtcaaca aaaaatcagc tcaagggaag tcatgcccag tcgatgaata
     3541 gagtatgggc gaagtaaaag caagcctccc ctcagatgtc tttctggttc agaaagcact
     3601 gggaatcgaa atggaaaaac gggcagccat tctgtctgca gatggacgag aattgcagag
     3661 aaagacccga gaatccgaag aagtgctccg ccagatgggg caaatggcag cttcgcctaa
     3721 gcctctgtcc cgggaagcca ttcgactcgc tgaaaaactg agagaagaat ccggagagaa
     3781 ccagagactc ctcgaaaaag caaatcatac gatccatcgc ctcctgacag aaatgggcga
     3841 aaaagagtcc acatacgatc cgcacgctcg caagcaacag tctacgggcg gcgtgctact
     3901 ggatgccagc gtctgatcaa tttttttgat tggatttttc aaatcagaac cgttgtctgt
     3961 ctttaagtgg aagagcgaat gagaatcaag ctctggggtg ttcgtggctc ccttcccaca
     4021 cccatgggtg tgcaggagta ccagaccatc ctcggtcatg ctctcgccca tgcgcagaat
     4081 gattttcgcg caaacccatc tattaccgca gagcaactct acgaaggaat tccagctcaa
     4141 taccgtcgcg tcataggc
//
LOCUS       DAORFF010000348         4154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12785_length_4154_cov_3.840449, whole genome shotgun sequence.
ACCESSION   DAORFF010000348 DAORFF010000000
VERSION     DAORFF010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12785_length_4154_cov_3.840449"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..524
                     /gene="rpsL"
                     /locus_tag="PKK76_13245"
     CDS             150..524
                     /gene="rpsL"
                     /locus_tag="PKK76_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNL69491.1"
                     /translation="MPTISQLIRHGRKQQQNRTKSPALASSPQRRGVCTRVMTFTPKK
                     PNSALRKVARVRLTTGIEITAYIPGEGHNLQEHNVVLVRGGRIKDLPGVRYHIVRGAL
                     DTLGVDKRRQGRSKYGAKRPKA"
     gene            528..1001
                     /gene="rpsG"
                     /locus_tag="PKK76_13250"
     CDS             528..1001
                     /gene="rpsG"
                     /locus_tag="PKK76_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNL69492.1"
                     /translation="MSRRTGSSHKRSIIPDPVYNDEDIARFINYMMKDGKKSTAEKVF
                     YAALDILKEKTGREAVDVFREAVQNVKPELEVRSRRVGGVTYQVPVEVRPERMRTLAV
                     RWIVQNARDRKDHSMAEKLAYELLDAQKGSGGAVKKRDETRRMAEANKAFSHYRW"
     gene            1017..3119
                     /gene="fusA"
                     /locus_tag="PKK76_13255"
     CDS             1017..3119
                     /gene="fusA"
                     /locus_tag="PKK76_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNL69493.1"
                     /translation="MAARKVSLEKTRNIGIMAHIDAGKTTTTERILYYTGRTYKIGEV
                     HEGSTEMDWMEQEKERGITITSAATTTFWNGADKDREPHRINIIDTPGHVDFTVEVER
                     SLRVLDGAVAVYDGVAGVEPQTETVWRQADRYHVPRICFVNKLDRMGADFFFCVNSIR
                     ERLGANGVPLQLPIGKEGGFKGLVDLVENRAIIWNGEELGAKFEYTEIPDEFKEQAKE
                     YRHQLMESAAECDDELLEKYLESGDLTVPELKRAIRAGTLKMKIFPILCGSAFKNKGV
                     QPMLDAVLDFLPSPLDIPPVVGLEVDGDKEIIRRADDKEPFAALAFKIMSDPYVGKLT
                     YFRVYSGVLKKGSYVMNSSKDKRERIGRLLQMHANERDEIEEVYAGDIAAAVGLKDTT
                     TGDTLCDEATPVVLERMSFPEPVIDLAIEPLTKADQEKLGMALMRLSEEDPTFRVKTD
                     RETGQTIISGMGELHLEIIVDRMKREFKVEANVGKPQVAYRETIRQQAEEEGKYIRQT
                     GGRGQYGHCWLRVMPNEPGKGYEFVNKVVGGTVPREYIPAIDKGCNEALQSGVLAGYP
                     MVDVKVEVFDGSYHDVDSSEMAFKIAASMGFKEACRKAKPVLLEPVMSVEVVTPEDYM
                     GEVVGDLNRRRGRINAMEQRGNARVVQADVPLAEMFGYATDLRSATQGRAAYTMQFKH
                     YEEVPNSVAQEIMAKAAV"
     gene            3215..>4154
                     /gene="tuf"
                     /locus_tag="PKK76_13260"
     CDS             3215..>4154
                     /gene="tuf"
                     /locus_tag="PKK76_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNL69494.1"
                     /translation="MAKEKFDRSKTHVNVGTIGHVDHGKTTLTAAITTVLAKKFGGKN
                     KAVAYDQIDNAPEEKARGITIATSHQEYESEKRHYAHVDCPGHADYVKNMITGAAQMD
                     AAILVVSATDGAMPQTREHILLARQVGVPYIVVFLNKVDMIAEGEREEMVELVQEEVK
                     ELLVKYGYPAETPFVRGSGLKALEGEDSELGTQAIIKLVETLDTYVPDPKRVLDKDFL
                     MPVEDVFTITGRGTVVTGRVEQGKISVNEEVEIVGIKDTVKTVVTGIEMFRKLLDHGQ
                     AGDNVGLLLRGIKKEDVERGQVVAKPGSITPHKKFKA"
BASE COUNT         1150 a          984 c         1122 g          898 t
ORIGIN      
        1 atggccctgc gttgttctgg gcttaggccc cgcgggaaac cgcatctgcc agggaagact
       61 gttcctcaca ggatgaggca ataagttccg ggcttattaa aaaatgaaaa acgctgaatt
      121 cggtagagat accggtatgg gagaagattt tgccaacgat cagtcaattg atccgccacg
      181 ggcggaaaca gcagcagaat agaaccaagt ctccggcact tgcaagttcg ccacagagac
      241 gcggggtctg cacgcgagtc atgacattca ctccaaaaaa gccaaactct gcgcttcgca
      301 aagtggctcg ggtgcgtctg acaactggaa tcgaaattac tgcttatatt ccgggtgaag
      361 gccacaacct tcaagagcac aacgtcgtgc tcgtccgcgg gggcagaatc aaggaccttc
      421 ccggtgtgcg ttatcacatt gttcgtggtg cgctcgatac cctcggggta gacaagcgtc
      481 gtcaaggacg ctcgaagtat ggggccaaga ggccaaaggc ttaaggtatg tcacgtagaa
      541 caggtagctc tcataaaaga tccatcatcc cggatccagt atacaacgac gaagatattg
      601 cacgtttcat caattacatg atgaaggatg gcaagaagtc aacggctgaa aaagtattct
      661 atgctgctct tgatattctc aaagagaaga ctggacgcga agctgtggat gtattccgcg
      721 aagcagttca gaatgtgaag ccggaactcg aagtccgttc ccgccgcgtt ggtggtgtaa
      781 cgtatcaggt tccagtggaa gttcgtccgg agcgcatgcg cactcttgcg gttcgttgga
      841 ttgtgcagaa cgcgcgcgat cgcaaagatc attccatggc agagaaactt gcttacgaac
      901 tgctcgatgc tcagaaaggc agcggtggcg cagttaagaa gcgcgacgaa acaagaagaa
      961 tggcagaggc aaacaaggcc ttcagccact acagatggta ataaggattt aattcaatgg
     1021 ctgctcgcaa ggtttcgctc gaaaagacac gtaacatcgg catcatggct cacattgatg
     1081 ccggtaagac cactactaca gaacgtattc tgtattacac cggtcgtact tacaagattg
     1141 gtgaggttca tgaaggttcc actgaaatgg actggatgga gcaagagaaa gaacgtggga
     1201 tcacgattac ttctgctgct acgaccacat tctggaatgg agcggacaag gaccgcgagc
     1261 cgcatcgcat caacatcatt gatacgcccg gccacgtaga ctttactgta gaagtagagc
     1321 gttctcttcg cgtcctcgac ggcgcggttg ccgtgtacga cggtgtggcc ggtgtagagc
     1381 cgcagacaga aacggtatgg cgtcaggcgg accgctatca tgttccacgc atttgttttg
     1441 taaacaaact cgatcgtatg ggagcagact tctttttctg cgtaaattcc attcgcgaac
     1501 gtcttggtgc aaacggtgtt cctctccagc tgccaatcgg caaagagggt gggttcaaag
     1561 gactcgtgga cctcgttgaa aaccgcgcga tcatctggaa cggtgaggaa ctcggcgcga
     1621 aattcgaata cactgaaatt ccagacgaat tcaaagagca ggcaaaagaa tatcgccatc
     1681 aactgatgga atctgctgca gagtgcgatg acgagctgct cgaaaaatat cttgagtcag
     1741 gcgatctaac agttcctgaa ctcaagcgag cgattcgtgc gggaacactg aagatgaaga
     1801 tcttcccaat tctctgcgga tctgctttca aaaacaaagg cgttcagcca atgctcgatg
     1861 cggttctgga tttccttcca tctcctctgg atattccacc tgttgtgggt ctcgaagtag
     1921 acggagacaa agagatcatt cgccgtgcgg atgacaaaga gccgtttgct gctctcgcgt
     1981 tcaagatcat gtctgaccca tatgtgggca agctcactta cttccgcgta tactccggtg
     2041 ttctgaaaaa gggttcttat gtaatgaact ccagcaagga caagcgcgag cggatcggac
     2101 gactgcttca aatgcatgcg aacgaacgcg atgaaatcga agaagtatat gcgggtgata
     2161 tcgcagctgc ggttggtctg aaagatacta ccacaggtga tactctctgc gatgaagcca
     2221 ctccagtggt tctggaaaga atgagcttcc ctgagcctgt cattgatctc gcaatcgagc
     2281 ctcttacaaa agcggatcag gaaaaactcg gcatggcgct catgcgtctg tctgaagaag
     2341 atccaacctt ccgcgtgaag acagaccgcg aaaccggtca gaccatcatc tccggtatgg
     2401 gtgaacttca cctcgagatc attgtcgatc gtatgaagcg tgaattcaag gttgaggcaa
     2461 acgtaggtaa gcctcaggtt gcataccgcg aaacgattcg ccagcaggca gaagaagaag
     2521 gcaaatacat ccgtcagaca ggtggtcgtg gtcaatacgg tcattgctgg ctgcgtgtga
     2581 tgccaaacga acccggcaaa ggttacgaat tcgtaaacaa ggtggtcggt ggaacagttc
     2641 ctcgcgaata cattccagcc atcgacaaag gctgtaacga agcgctgcag agcggcgtcc
     2701 tggccggcta tccaatggta gacgtgaaag tcgaagtatt cgacggaagc taccatgatg
     2761 tggactcttc tgaaatggcg ttcaagatcg cagcttccat gggcttcaaa gaagcctgcc
     2821 gtaaggcaaa gccggtcttg ctcgaaccag tcatgagcgt cgaagttgta actccagaag
     2881 actacatggg cgaagtagta ggtgacctga accgcagacg cggtcggatc aatgctatgg
     2941 agcagcgcgg aaatgcccgc gtagtgcagg cagatgtgcc gctggccgaa atgttcggtt
     3001 atgcgacaga tctacggagt gcaacccagg gtcgtgcggc ctatactatg cagttcaagc
     3061 actacgaaga agtaccaaac agcgtagcgc aggaaatcat ggcgaaggcg gcggtttaag
     3121 cgcaaaaatc aatagacggc tgggcccgat tttgaaaatc tggccttccg acttttctaa
     3181 aggaatacta caaaggataa gataggagaa ggtcatggca aaagagaaat ttgataggtc
     3241 aaaaacacac gtaaacgttg gcacaatcgg ccacgttgac cacggcaaga caacactgac
     3301 ggcagcaatt actactgtac ttgcaaagaa gttcggcggt aagaacaaag ctgttgcgta
     3361 cgatcaaatc gataacgcac cagaagaaaa agcacgtggg atcaccattg ccacttctca
     3421 ccaggaatac gaatcagaaa agcgccacta cgcgcacgta gactgtccag gtcatgctga
     3481 ctatgtgaag aacatgatca cgggtgcagc tcagatggac gcggcgattc tcgttgtttc
     3541 cgcaactgac ggtgcaatgc ctcagactcg cgagcacatc ctgctcgctc gtcaggttgg
     3601 tgttccttac atcgttgtat tcctcaacaa agtagacatg atcgctgaag gcgaacgtga
     3661 agaaatggtt gaactcgttc aggaagaagt gaaggaactg ctcgttaagt acggctatcc
     3721 tgctgaaact cctttcgttc gcggttcagg cctcaaggct ctcgaaggcg aagacagcga
     3781 gcttggaact caggcaatca tcaaactggt tgaaactctc gacacttatg ttccagatcc
     3841 aaagcgcgtt ctggataaag acttcctgat gccagtagaa gacgtgttca ccattaccgg
     3901 tcgtggtact gtagtaacag gtcgcgtgga gcagggcaaa atctctgtga acgaagaagt
     3961 tgaaatcgtc ggtatcaaag acactgttaa gacagttgta accggtattg aaatgttccg
     4021 taagcttctc gatcacggtc aggcgggaga caacgttggt cttcttctcc gtggcatcaa
     4081 gaaagaagac gtggagcgcg gccaggtggt tgcaaagcca gggtccatca ctcctcacaa
     4141 aaagttcaag gcag
//
LOCUS       DAORFF010000349         4149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12808_length_4149_cov_3.676111, whole genome shotgun sequence.
ACCESSION   DAORFF010000349 DAORFF010000000
VERSION     DAORFF010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12808_length_4149_cov_3.676111"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1485)
                     /gene="nusA"
                     /locus_tag="PKK76_13265"
     CDS             complement(133..1485)
                     /gene="nusA"
                     /locus_tag="PKK76_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNL69495.1"
                     /translation="MARATKKKTNQEPDIKAFFDLLTEIATEKGLSRDEVVEVFKGSL
                     ITAYQKKYGLDANLEVILDREKPEIAVICRRKVVKPVLNTAQEISPEEAAKIEPGIKE
                     GDILSVTENPFEFSRIGATNVRQILLQRLKELEREILYNEFKAKENELVNGYFLRWRD
                     KDLMYVDLGKIEGVMPRREQIPNEKFRPGDRVKAVIKLVELRREKTREPGPFVTLSRG
                     SGEFVKKLFEMEIPEIYDGVVEITGIARYSGYRTKILVKSNRMDVDPVGACVGVRGVR
                     IQSVVRELGNERIDIVNDNGSPADIIASAMSPAVVTEVRADANTREALVLVPDDQQSK
                     AIGNGGQNVRLASQLTGYKLTVKTLSQFNEEMASPEARARLEQLFSQAQPEPEEEYTP
                     LSELPDLSSRIQSLLEAAGIKSVEQLVEADIEDLGKILGPATARHVQKVVSEAVEVTE
                     "
     gene            complement(1488..1994)
                     /locus_tag="PKK76_13270"
     CDS             complement(1488..1994)
                     /locus_tag="PKK76_13270"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69496.1"
                     /translation="MIGELATQALGPGLAVFSANMHPVKSGFHIQVELDGLSHPQGAV
                     SVGDCEEFSKRLSNLIDQAVERQEPGVPAGFTAENYTLEVSSAGAERELRLPQDLLRF
                     KEVPIKLRYEEDGKEDTAIVTSAGKGEDGLYLFLAFKGRRKQRKQKFKELRLEESKIR
                     KANLFLDF"
     gene            2168..3259
                     /locus_tag="PKK76_13275"
     CDS             2168..3259
                     /locus_tag="PKK76_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69497.1"
                     /translation="MHIRVAACILLTLAGCARFRVQELKPTRVFAVPLLSAFPHLAKG
                     SDEFTRPIQIAGITSSPAILGNRIFIPDPTAGMVRIFKRNADTADSVVADKLEKGSEA
                     TLIRVRAGIPGRAVSDDDENVFIVLHEGPAPDEKNEPEPPPERRTTDRFNANAFMDTP
                     ARIVHIKEDQLAGIFGREGGKEGPAFERILRLHAIEGALYVFHSVGGARQLSVYQNEK
                     RIKQFENFTIPENDALKYFTDIEDMFPAGQSVIVSVVLRNKNTFAPEARRVYSWDGHT
                     ANLIYTIDEDADQFIAARPDGGFTVANSEDGNRMLLKIYSPSGEYLNNRLIVMPDFRN
                     TWREVTVDAAGRVFSNRIYKGSFEVYEWK"
     gene            3246..>4149
                     /locus_tag="PKK76_13280"
     CDS             3246..>4149
                     /locus_tag="PKK76_13280"
                     /inference="COORDINATES: protein motif:HMM:NF015791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNL69498.1"
                     /translation="MNGNRPLLTYAQSRTLDLDTIAAGFTEQQLMGQAAVASVNCLLS
                     DGTLDSHETILILCGKGNNGGDGYAIAWALIGAGFSSRVQIFAAEPPSSQSALFYRRM
                     CEKHTPIQDLTHFAQKASGPGDLILECLLGSGQKGDLRPPYLESAMDIARACKRGATL
                     IAVDCPVGLIEERTASFGEELPLPDEVHCYGPIKLAAALNVSLATGSLVRSLPIGFEP
                     VESSAWLWDGPPVQFHKSSIDHKYSAGSAAILGGEPGMEGAAILASDSFFAAGGGICE
                     VLTTSAESRSRILSARPSLMVRTLE"
BASE COUNT          956 a         1121 c         1009 g         1063 t
ORIGIN      
        1 gcgcgattcc gccttctttg gctccgcttt gttcgagaaa gtatcagaaa tatcacgagc
       61 cttctggcct tctaccagtg gcttcttgat cgtgattttc ttcttggccg gctggctctt
      121 gttatctgac atttactcgg ttacctctac tgcttctgac acaaccttct gcacgtgacg
      181 ggcagtggcc ggaccaagaa tcttaccaag atcctcgata tctgcttcca ccagttgctc
      241 tacactctta atacctgccg cttccagaag cgattggatg cgtgaggaaa gatcaggcag
      301 ctctgaaagg ggggtgtatt cctcttctgg ctcgggctgt gcctggctga acaactgttc
      361 cagcctggct ctggcctctg gagatgccat ctcttcgttg aactgagaaa gcgttttcac
      421 ggtcagctta taacctgtaa gctgggatgc aagccttaca ttctgcccac cattgccgat
      481 cgccttgctt tgctgatcgt ctggaacaag aaccagagct tcgcgggtgt tcgcatccgc
      541 tctcacttct gtgaccacag caggagacat agcgcttgcg atgatgtcag cggggcttcc
      601 gttgtcgttt acgatgtcga ttctctcgtt tccaagctct cgcaccacgg attgaatgcg
      661 aactccgcga acgccaacgc atgcgcccac tggatccaca tccatacgat tggatttcac
      721 aagaatcttc gtgcgatagc cggaatacct ggcgatgcca gtgatctcaa ctacgccgtc
      781 atagatttcc gggatctcca tttcgaatag tttcttaaca aactcacctg aaccgcgaga
      841 aagagttaca aacggacccg gctcacgcgt tttttcgcga cgcagttcta ccagtttgat
      901 gactgctttt acacgatctc ctggacgaaa cttttcgttc gggatttgct cacgacgagg
      961 catgactccc tcgattttgc cgaggtccac atacatcagg tccttatcgc gccagcgaag
     1021 gaagtaacca tttacgagtt cgttttcttt tgccttgaat tcattgtata agatttcgcg
     1081 ttcgagctct ttgaggcgct gtagtaagat ctgacgaaca ttggttgcac cgatgcgcga
     1141 gaattcaaaa ggattctcag tgacagatag aatgtctccc tctttgattc ctggttcgat
     1201 ctttgccgct tcttctggag aaatttcttg cgctgtgttc agtacaggtt tgactacctt
     1261 gcgacggcag atcaccgcga tttctggttt ttcgcgatcc agaatcactt cgaggttcgc
     1321 gtccaggccg tacttcttct gatacgctgt gatgagactg cctttgaata cttccaccac
     1381 ttcgtcgcgc gaaagtccct tctctgttgc aatctctgtg agaagatcaa agaatgcttt
     1441 gatatcaggt tcttgattcg ttttcttttt cgtagctctt gccatattca aaaatccaga
     1501 aatagattcg cttttcgaat cttcgactct tcgagtcgaa gttctttgaa cttctgtttt
     1561 ctctgcttcc ttctgccttt gaaggccaga aacaaataga gtccgtcttc ccccttcccg
     1621 gcacttgtca cgatcgccgt gtcctctttc ccatcttctt cgtaacgaag cttgatggga
     1681 acttccttga accgcagaag atcctgggga agcctgagct cgcgctctgc tccagccgag
     1741 gaaacctcga gcgtgtagtt ctccgcagtg aacccggctg gaacacctgg ttcctgccgc
     1801 tccactgcct gatcgattag attagacagc cgctttgaga actcttcaca gtcaccgaca
     1861 gaaacggcac cctgagggtg cgatagaccg tctagctcca cctggatgtg aaagccgctt
     1921 ttgaccgggt gcatgttcgc gctgaagacc gcaagccccg gcccaagcgc ctgggttgca
     1981 agttccccga tcatttttcc gcctgtgacc aaagaaaagt ggaaattccg cggaggcaga
     2041 tcccactctt caatcctgtc cagaaaacct gaacactggc ctggcgtcaa gtcaaaggct
     2101 gcatgacttc cagctcgcaa aaaggattca tccttgcctt ggttgcacga actccaaaag
     2161 tcgcctcgtg cacatccgtg ttgcagcctg tatccttctc acacttgccg ggtgcgcgcg
     2221 attccgcgtt caagaactca aaccaacgcg cgtattcgca gtgccactcc tatctgcatt
     2281 tcctcatctg gcaaaaggtt ctgacgaatt cactcgcccc atacaaattg cagggatcac
     2341 atcgagcccg gcgatcctcg gaaaccgcat cttcatcccg gatccaactg cgggcatggt
     2401 gagaatattc aaacgaaatg cagacactgc agattcggtc gtagcagaca agcttgagaa
     2461 aggatcggaa gccacactca ttcgagtgcg tgctggaatt ccagggcgcg ccgtttcaga
     2521 cgatgatgaa aacgtcttca ttgtgctgca cgaagggcct gctccggatg aaaagaacga
     2581 gcccgaacct ccgccagagc gcagaaccac agacagattc aacgcaaatg cttttatgga
     2641 cacgccggca aggatcgttc atatcaaaga agatcagctg gccgggattt ttggacgaga
     2701 gggcggaaaa gaaggtcctg cgtttgagcg aattcttcgc ctccacgcca ttgaaggtgc
     2761 cctctatgtc tttcattctg tggggggagc gcgacagctt tctgtttacc agaacgaaaa
     2821 gagaatcaaa cagtttgaaa actttactat cccggaaaac gatgcgctga aatactttac
     2881 agatatagag gatatgtttc ctgcgggcca atccgtaatc gtaagcgttg tgctcagaaa
     2941 caaaaacaca tttgcgccag aagccagaag agtgtattcc tgggatggcc acactgcaaa
     3001 tctgatctac acgattgatg aagacgcaga tcaattcatc gccgcgcggc cggacggagg
     3061 tttcactgtt gcaaactccg aagacggaaa tcgcatgctt ctcaaaatct attctccgtc
     3121 cggtgaatat ctaaacaaca gactcatcgt aatgcctgac ttcagaaaca cgtggcgcga
     3181 agtgacagtg gacgcagccg gcagagtttt ctccaatcgc atctacaaag gaagcttcga
     3241 ggtctatgaa tggaaatagg ccactcctta cctatgcgca atcgcgcact ctagatttag
     3301 atacaatcgc cgccggattc acagaacagc aactgatggg acaggccgca gtcgcgagcg
     3361 tcaactgcct gctttcggat ggaacgctgg attcgcacga aaccattttg attctctgtg
     3421 gaaagggaaa caacggtggc gacggttatg caattgcctg ggcattgata ggagctggat
     3481 tctcatcgcg ggttcagata tttgcagcag agccgccctc ttctcaatct gctcttttct
     3541 atagaagaat gtgcgaaaag catacgccga ttcaggatct aactcatttc gcacagaaag
     3601 catccggccc gggcgatttg attctggaat gcctgctcgg gtcaggacag aagggtgatc
     3661 ttcgcccgcc ttacctggaa tccgccatgg acattgcgcg cgcgtgcaag agaggagcca
     3721 ctctcattgc agtggactgc cccgttggac tcattgaaga aagaaccgct tctttcggag
     3781 aggaacttcc tctgccagac gaagttcatt gctacgggcc tatcaaactg gcagctgctc
     3841 tcaacgtgtc gcttgctact ggatctctgg ttcgcagtct gccgatcggg tttgagcctg
     3901 tcgaatccag tgcgtggctc tgggacggac cacccgttca atttcataaa tcatctatag
     3961 atcacaagta ttctgccggg agcgctgcga tccttggagg cgaaccggga atggagggcg
     4021 ctgccattct tgcttccgac tctttttttg cggcaggagg tggaatctgt gaagtgctca
     4081 ctacttctgc ggaatcccgg tcgcggattc tatccgccag gccttctctg atggttcgca
     4141 cactcgaag
//
LOCUS       DAORFF010000350         4147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12824_length_4147_cov_3.085777, whole genome shotgun sequence.
ACCESSION   DAORFF010000350 DAORFF010000000
VERSION     DAORFF010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12824_length_4147_cov_3.085777"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PKK76_13285"
     CDS             complement(<1..273)
                     /locus_tag="PKK76_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69499.1"
                     /translation="MPSSEMDFTFMKQRIEDAVTDNLKTITNLKVDAGAAVKEILSYV
                     FRNVNPDSVLADHARYDWYYSLLGKTIAIAQGWKINHAPEVKSAAET"
     gene            complement(319..2442)
                     /locus_tag="PKK76_13290"
     CDS             complement(319..2442)
                     /locus_tag="PKK76_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HNL69500.1"
                     /translation="MGTVYRSCTLCEAICGLEIKVEDGVIKGIRGDSADVFSHGHICP
                     KGPELKAIHEDPDRLKFPLKRVGDKWEQVAWETAFSEIAARLHVIQTASGQDSVAYYA
                     GNPNVHNYGSLLFAPRLMKAIKTRNIYSATSVDQLPHHFAALKMFGHPLLVPVPDVDR
                     TDYFLILGANPMASNGSMMTAPDMRGRMKAIQKRGGKIVVVDPRKTETADLADEHVFV
                     RPGKDVYFLLSILHVVLAGEKKKTSRLESIFQGADFVRSLVQDYSPEKTQAHTGISAE
                     TTRRIATQFAQAKSAVAYGRIGVSTQTFGGLCHWLINVLNAITGNLDEPGGAMFSLPA
                     VDTIAAEGKRESFGSFDSYRSKARNLPEFGGELPVSALADEILFQGEGGVRALITSAG
                     NPVLSTPNGGKLAQALESLQLMVSIDIYLNETTKYAHYILPPTSPLEHDHYDIVFNML
                     AVRNTARYSPPVFDPPAGTMHDWEIFTALTAALEEARSGGVKKASSARAKIKPTDFLD
                     RMLKSGPYGSQGLNFQKLVENPHGVDFGPLVPVLPARLKTIDGQIDLAPDLMRRDIER
                     AREAMKETPAGMILIGRRNLRDNNSWMHNVPRLMNGSPRCTLFIHPEDAAKFGISDGH
                     EAIVRSRVGEIRLPVEIVEEIMPGVVSIPHGYGHKKSGTKMSTAQKHAGVSINDLTDD
                     SSLDELTGNAAFNGVPVEIRAASVS"
     gene            complement(2445..>4147)
                     /locus_tag="PKK76_13295"
     CDS             complement(2445..>4147)
                     /locus_tag="PKK76_13295"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNL69501.1"
                     /translation="QHDEEKLVRPVGHQGGDKDYLTSIRVTWDDSEHGMGPTGMAIKT
                     GLCHVNQDVYTNPAMAPWRKEAISRGYISTIALPLQDASGTFGALNIYAGEANAFDDD
                     EIELLRKLAADLSFGVEALRTREKRRRAEEAVHRLAYFDTLTGLPNRTQLLASLTNGI
                     AESALKAQSLALLFFNIDHFTEFQDGLGVRQAEALLQKIALRLQSAAGSNFLARLDGD
                     RFAMIHPNATADSISALAVRVHRAMEESIVQSDVRIDVRINLGAAMYPDHGADADSLL
                     LRSDIACRRAKALGVSHMMYSGATDQESPHRLSLIADLRRAIQSDQLILHFQPKIDSR
                     TGVISGAEALVRWSHPVRGLISPAEFIPLAEHTGLIAPLTDWVLRAAVRESAAWLAKG
                     IAVPIAVNVSPQNLRDDDLIDRISTLLQEARVPAENLQLEITETTLMEDPARSFELLS
                     RLKKQGIRIFVDDFGTGYSSLSYIATLPLYSLKIDRSFVVKMTRSKEHRAIVAAAISL
                     AHSLGLRVVAEGVDAVDQLADLKELGCDEIQGFLFSKPLSSKDFLSWSVDFSNTAVRR
                     "
BASE COUNT         1028 a         1105 c         1017 g          997 t
ORIGIN      
        1 tgtttccgct gcagatttca cttctggcgc atggttgatt ttccaaccct gagcaatggc
       61 gatggtcttt ccaaggagtg aataatacca atcgtaacgc gcatgatccg caagcacaga
      121 atcgggattt acgttcctga aaacatacga gagaatttct ttgactgccg ctcctgcatc
      181 caccttgaga ttcgtaatgg tctttagatt gtccgtgacc gcatcttcaa tgcgctgctt
      241 catgaatgta aaatccattt cagatgacgg catagagcga tggggaatga ttcgcagttc
      301 ccgtccagca aaacgcaatc aagaaacaga agcagcgcgg atctccacag gtacaccatt
      361 aaaggcggca ttgcctgtga gctcatccag ggatgaatcg tccgttaaat cattgatgct
      421 cactccagcg tgcttctgag ccgtgctcat ctttgtgcca gatttcttgt gtccataccc
      481 atgcgggatg ctcacgacac caggcatgat ttcctctaca atctcaacgg gcagcctgat
      541 ttcgccgaca cgagatcgaa caatcgcttc gtggccgtct gaaattccaa acttcgcagc
      601 atcctccgga tgaatgaaaa gagtgcatcg tggtgaaccg ttcatcaggc gcggtacatt
      661 gtgcatccac gaattgttgt cgcgaaggtt ccttctgccg atcaaaatca ttccggcagg
      721 cgtttccttc atggcttcgc gcgcacgctc tatgtcgcgc ctcatcaaat ccggagccag
      781 atcaatctgg ccgtctattg ttttgaggcg agctggcagg acgggcacta gaggtccgaa
      841 atcaactccg tgcggattct caaccaactt ttgaaaattg agcccttggc ttccgtaagg
      901 tccggatttc aacatacgat ccaggaaatc agtgggtttg atcttcgctc ttgctgacga
      961 agctttcttt accccgccgc tgcgagcctc ctccagagct gctgtgagtg cagtaaagat
     1021 ctcccagtca tgcatggtgc ctgcgggcgg atcaaataca ggaggggagt aacgagcggt
     1081 atttctcact gccagcatgt tgaatacaat gtcgtaatga tcgtgttcga gaggagaagt
     1141 aggcggcaaa atgtagtgag cgtacttcgt tgtttcattc agatagatgt caatgctcac
     1201 catcagttgc agtgattcaa gtgcctgcgc cagcttaccg ccgttaggtg tagaaagcac
     1261 gggattgccg gcggaagtaa tcaaagcgcg aacgccgccc tctccctgaa acaagatctc
     1321 atctgccagg gcagacacag gaagctctcc tccgaactcc ggtaaattgc gagccttact
     1381 cctgtatgaa tcaaaacttc caaaggactc ccgctttcct tctgcggcaa tggtatccac
     1441 ggccggcaaa ctgaacatcg ctccacctgg ctcatccaga ttgccagtga tagcgttcaa
     1501 aacattgatg agccagtgac agagaccgcc aaacgtttga gtcgaaactc cgattcggcc
     1561 ataagccacg gctgattttg cctgcgcaaa ttgagtggca atccgcctcg tggtttccgc
     1621 agaaattcct gtgtgagcct gggttttttc tggagagtaa tcctgaacca ggcttcgcac
     1681 aaagtcggca ccctggaaga tgctttcgag tctggaggtc ttcttctttt ctcctgcaag
     1741 cacaacgtga agaatcgaaa gtaagaaata aacgtctttg cccggacgaa caaagacatg
     1801 ctcgtctgcc aggtccgccg tttccgtttt gcgcggatct actacgacaa ttttcccacc
     1861 gcgcttctga attgctttca ttctgccgcg catgtcagga gcagtcatca tactaccgtt
     1921 agacgccatt gggttagcac ccagaatcag aaaatagtct gttcgatcca catctggcac
     1981 agggacgaga agaggatgcc cgaacatttt gagagcagcg aagtgatgcg gaagctgatc
     2041 tacggaagtg gcagaataga tgttcctggt cttgatcgcc ttcatgagcc gcggtgcaaa
     2101 cagaagagaa ccgtaattat gtacgtttgg atttccggca tagtaggcaa cggaatcctg
     2161 tccgcttgcg gtctggatga catgcaatct ggccgcaatt tcggaaaatg cagtttccca
     2221 cgcgacctgc tcccatttgt ctcctactct cttcagtgga aatttcagac gatctggatc
     2281 ctcatgaatt gctttgagtt cagggccctt gggacaaatg tgtccgtgcg aaaagacatc
     2341 cgcgctgtct ccgcgaattc ctttgatcac tccgtcttct actttgatct caagcccgca
     2401 aattgcttcg catagcgtgc acgaacgata cactgttccc atgatcatct cctaacggct
     2461 gtattcgaga aatcgacact ccaggacaaa aaatcttttg aagacagtgg ctttgaaaat
     2521 agaaatccct ggatctcatc gcatccaagt tccttcaaat cagccagctg atccacagcg
     2581 tccacaccct ctgccaccac tcgcaaaccc agagaatgcg ccagagaaat ggccgcggcc
     2641 acaatcgcac ggtgttcttt gctcctggtc atcttgacca caaaggaacg atcaatcttc
     2701 agggaataca aaggaagggt tgcaatgtag ctgagagaag agtagccagt gccaaaatcg
     2761 tccacgaaga ttcgaatgcc ctgcttctta aggcgcgaaa gcaattcaaa ggatcgagcg
     2821 ggatcttcca tcagagtagt ttctgtgatt tccagctgca aattttcagc cggaacgcgc
     2881 gcttcttgaa gcaatgtaga aatgcgatca atcaaatcgt cgtcgcgcaa gttttgtgga
     2941 gacacgttga ctgcgatggg aactgcaatt ccttttgcaa gccacgctgc gctctctctt
     3001 accgcagcac gcaaaaccca atcggtcaga ggagcaatca ggccagtgtg ttctgcgagc
     3061 gggatgaatt cagcaggtga tataagtccg cgaactggat gagaccagcg aaccagggct
     3121 tctgctccac tgatgacacc cgtgcgagaa tcgatctttg gctgaaaatg caaaatgagc
     3181 tgatcggatt gaatggcacg cctgaggtct gcgatgagag aaagcctgtg aggactttcc
     3241 tggtccgtgg cgccactgta catcatatgc gaaacgccca gcgctttcgc gcgacggcat
     3301 gctatatcgc tgcgcaaaag caaggaatcc gcgtctgcac cgtggtcggg gtacatagcc
     3361 gcacccagat tgatgcgcac atcaatgcga acgtcactct ggacaatgga ttcttccatg
     3421 gctctgtgaa cgcgcacggc cagagcagag atggagtctg cggtggcatt cggatgaatc
     3481 atcgcaaacc gatctccatc cagtctggcc aggaaattgg atcctgcggc agattgaagt
     3541 ctaagagcga tcttctgcaa aagcgcttcc gcctgacgaa ctcccagccc atcctgaaac
     3601 tcggtaaaat gatctatatt aaagaataac aaagcaagag actgcgcctt gagtgcggat
     3661 tccgcaattc cattggtcaa actcgcaagc agctgcgttc gattgggcag accggtgaga
     3721 gtgtcaaaat acgcaagcct gtgaacggcc tcctctgccc ggcgtctttt ttcgcgcgta
     3781 cgtagcgcct ccactccaaa actcagatcc gccgcgagct tgcgcagcag ttctatttca
     3841 tcatcatcaa aggcattggc ttcccccgcg taaatattca gtgccccgaa cgtgccggaa
     3901 gcatcttgca gcggtaaggc gatcgtagaa atataacccc tggaaatcgc ttcttttctc
     3961 cagggtgcca tggctggatt ggtgtacaca tcctggttca catgacacag ccccgtcttg
     4021 attgccattc cggttggccc catgccgtgt tcagaatcgt cccaggttac gcgaatagaa
     4081 gttagataat ccttatctcc accctgatga ccaacagggc gcacaagctt ctcttcgtca
     4141 tgctgag
//
LOCUS       DAORFF010000351         4142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12852_length_4142_cov_2.925862, whole genome shotgun sequence.
ACCESSION   DAORFF010000351 DAORFF010000000
VERSION     DAORFF010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12852_length_4142_cov_2.925862"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1852
                     /locus_tag="PKK76_13300"
     CDS             <1..1852
                     /locus_tag="PKK76_13300"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL69502.1"
                     /translation="EFQKLPEEDQRAISEKIEKFGTTLQEVLRQFPVWRRETQNRLRD
                     VMRNTLGLAVGHIIDEIKDKFRDLPEVIAFLDQVLSDVVDSGPQARDAKEEEGSTISG
                     VPAPFQKYEVNLLVDRDGQKCAPVVYEDNPSYQNLIGRIDQVGHFGLLVTNFTMIRAG
                     SLHRANGGYLLVDGEKLLAQPFAYDSLKRALKSSTIRIESPGQLLAGISTSPLEPDTI
                     PLRVKVVLFCGRQLHYMLRELDPEFDELFKVAAEFESDVERTPETVSLYAQMLASLVR
                     TEKLRSFTREAFARIIEHSARLAGDANRLSARTRRLADLMRESDYFAGQAGRASVDVQ
                     DVMRSLKEAEDRRGRIREAIQREILRDTIQIATNGAAVGSINGLAVAIQGDAAFAHPV
                     RITAAVWPGKGDIIDIERETELGGQIHSKGVFILSAFLASRFSRTKPLSLSASLVFEQ
                     SYGPVEGDSASLAELCVLISAISGVPILQGIAMTGSVSQHGVAQAIGGASEKIEGFFD
                     ICRARGLTGSQGVIIPSSNIKHLMIRRDIQEACEAGTFHIYAVDHVDDAVAILTQLEP
                     GTADESGEFPADTVYGKLVSALDSMSRAGRRTRRQVSDPHSVDSDASDKK"
     gene            1926..2885
                     /gene="coxB"
                     /locus_tag="PKK76_13305"
     CDS             1926..2885
                     /gene="coxB"
                     /locus_tag="PKK76_13305"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNL69503.1"
                     /translation="MNFLPENIATYGTQIDGLAALISWLGLTSFVLAEAALVYAAFRY
                     RRSEKRKRAAYIKGESWGQAKWIFIPLILVVLLDIFIDIKTTAVWKQVKIDLPTADMN
                     VRIVGQQFNWQFTHPGKDGKLGTEDDVKTTNELHVPVDTNVVFELGAKDVLHSFFVPA
                     LRLTQDAVPGRFIHGWFNATKPGKYEIACGQICGTGHTGMRGMLIVHNKEEFQKWVEN
                     PVTGGSTDPMEILKGNGCTSCHSVDGTRVVGPSYKGIWGRSETVITDGAERQVVVDEA
                     YIKKSIREPKADTVKGYPAGVMPAGTLSDADIDVLIKYFKTLK"
     gene            2902..>4142
                     /locus_tag="PKK76_13310"
     CDS             2902..>4142
                     /locus_tag="PKK76_13310"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNL69504.1"
                     /translation="MSHAMHAAPTSFLRKYVFATDHKVIGMQYLITGILMALVGGFLS
                     YLFRMQLAFPGESIPGFGKLAASQYNIAITMHGTIMVFWVAMPILLAAFGNFLIPLMV
                     GADDMAFPTLNMMSYQVFFISTVILLVSFFVPGGAFAAGWTAYPPLSAGTYAGSKDFL
                     SNMGGHLWIIAIALEFIAFLMGGINFLVTSFNMRAPGLSWFRLPVVVWMLDLAVLLFM
                     FSIGPLLAGAIMLLFDRTVGTGFYDPAQGGDPILFQHLFWFFGHPEVYVMLLPTLGFL
                     CEILATFARKPIFGYKYILYFTFGAALLSFIVWAHHQFVSGINPRMANFFSLTTLLIS
                     VPFTVIVFASIATLWRGNIQFSVPMLWAVGFVAEFMIGGVTGVYLGSSAFDIYAHDTY
                     FVIAHFHYALIPTVVFGGFAA"
BASE COUNT          955 a         1049 c         1081 g         1057 t
ORIGIN      
        1 agaatttcag aagctgcccg aggaagacca gcgggcgatc agcgaaaaaa tagagaagtt
       61 cgggactacc ttgcaagagg ttctgcgcca gtttccagta tggcgtcgcg aaacacagaa
      121 tcgccttcgc gatgttatgc gaaatacgct cggtctggct gtaggtcata tcatcgatga
      181 aatcaaggac aagtttcgcg atttgccaga agtcattgct tttctcgatc aggttctttc
      241 ggacgtagtt gacagcggtc cacaggcacg cgacgcaaaa gaagaggaag ggtctactat
      301 ttctggcgtg cctgctcctt ttcagaaata cgaagtcaat cttctagtgg atcgcgacgg
      361 acagaaatgt gcccccgtgg tttatgaaga caatccatcc tatcagaact tgattgggcg
      421 aattgatcag gtgggtcatt ttggtcttct ggtgaccaac ttcaccatga ttcgtgccgg
      481 ctcccttcac agagcaaacg gcggttatct gcttgtggat ggagaaaaac tcctggctca
      541 accattcgcc tacgacagtc ttaagagagc actcaagtct tctacgattc gaattgaatc
      601 tcccgggcag cttctcgctg gaatttctac ttcacctcta gagccggaca ccattcctct
      661 tcgcgtgaaa gttgtgctgt tctgtgggcg gcagcttcac tacatgctgc gagaactgga
      721 tccggaattt gacgaattgt tcaaggtggc ggcggaattt gaaagtgatg tggagcgaac
      781 gcccgaaacc gtctccctct acgcgcaaat gcttgcatcc cttgttcgca cagaaaaact
      841 cagatcgttc acacgcgaag cgttcgctcg tatcattgaa cacagcgcca ggctggccgg
      901 ggacgcaaac agactcagcg cgcgcacgcg tcgtctggcg gatctgatgc gcgaatcaga
      961 ctacttcgcg gggcaggcag gcagagcttc cgtagacgta caggacgtta tgcgatctct
     1021 gaaagaagcg gaggaccgtc gcggtcgaat ccgcgaggca atccagagag aaattctccg
     1081 tgatacaatc cagatcgcca caaatggagc cgcagttggt tctatcaacg gtctggcggt
     1141 cgcgattcaa ggagatgctg ccttcgcgca ccccgtgcga attaccgccg cagtgtggcc
     1201 cggaaaagga gacatcatcg acattgagcg cgaaacggaa ctgggcggac agattcactc
     1261 gaaaggagta ttcattctct ccgctttcct tgcttccagg ttttcgcgca cgaagcctct
     1321 atctctctcc gccagtcttg tattcgaaca gtcctatggc cctgtagaag gggactctgc
     1381 gtccctggct gaattgtgcg ttctgatttc tgccatctcc ggagttccga tccttcaggg
     1441 aatcgccatg accggttcgg tgagccagca cggtgtcgcc caggcaattg gaggggccag
     1501 cgaaaaaata gaaggcttct ttgacatttg cagggcgaga ggtctcacag gcagtcaggg
     1561 agtgatcatt ccatcttcga atatcaaaca tttgatgatt cgccgcgaca tccaggaggc
     1621 ctgcgaagcc gggaccttcc acatctacgc cgtagaccat gtggacgatg cagtggcgat
     1681 tttgacgcag cttgagcctg gcactgcgga tgaaagcggg gaatttccgg cggacaccgt
     1741 ttacgggaag ctagtttccg cgcttgattc tatgtccagg gcaggacgtc gcacacggcg
     1801 tcaggtctca gaccctcatt ccgtggattc ggatgcgtca gacaaaaaat agttgctcca
     1861 taccctttgc gccatttgct gaactttagc cgcgtgttcc acgctttcat ggagagaacg
     1921 gatcaatgaa tttcttaccg gagaatattg caacctacgg aacgcagatt gacggactgg
     1981 cagccctcat cagctggctg ggtcttacca gctttgtgct cgctgaagcg gctctggtct
     2041 atgctgcatt tcgttatcgc agaagcgaaa agaggaagcg tgcagcatac atcaaagggg
     2101 aatcctgggg tcaggcaaag tggattttca ttcctctcat tctagtagtt ctcctggaca
     2161 tcttcattga tatcaagacc actgcggtgt ggaagcaagt gaagatcgat ctgccaacgg
     2221 cggatatgaa tgtgcgaatc gttggtcagc agttcaactg gcaattcact catcctggta
     2281 aggatggaaa actgggcaca gaagacgatg tgaagactac aaatgagctt catgttccag
     2341 tagatacaaa cgtagttttt gaactgggcg ccaaagacgt tctgcacagc tttttcgtgc
     2401 cagcacttcg cctcactcag gatgcggttc caggtcgttt cattcacgga tggttcaacg
     2461 caaccaaacc tggcaaatac gaaatcgcat gcgggcaaat ctgtggaacc ggccacacgg
     2521 gaatgcgcgg aatgctgatc gttcataata aagaagagtt tcaaaaatgg gtagagaatc
     2581 ctgtaacagg tggctctaca gatccaatgg aaatcctcaa aggaaacgga tgtacttcct
     2641 gtcactccgt ggacggaacc agagtcgtcg gcccaagtta caaaggtatc tggggtagat
     2701 cagagactgt gatcacagat ggcgcagagc gccaggtggt tgtagatgaa gcgtacatca
     2761 aaaaatcgat tcgtgaacca aaagcggaca cggtcaaagg atatccagcc ggtgtaatgc
     2821 ctgccggaac tctttcagac gcagacatcg atgttctaat caaatacttt aagacactga
     2881 agtaaggaaa gggagaaaga aatgagtcac gcaatgcatg cagcgcccac aagcttcctg
     2941 cgcaaatacg tcttcgcaac cgatcacaaa gtcattggaa tgcaatacct gatcacaggg
     3001 attctgatgg cgctggtggg cggctttctg tcctatctct tccgtatgca gcttgcattt
     3061 ccaggagaaa gcattcctgg atttggtaag ctcgccgctt cgcagtacaa cattgccatc
     3121 accatgcatg gaaccatcat ggtattctgg gtggccatgc cgattctcct ggctgcattc
     3181 ggcaacttcc tgattccgct gatggtggga gcagacgaca tggcgtttcc gacgttgaac
     3241 atgatgtcgt atcaggtgtt cttcatcagt acagtcattc ttctcgtgtc tttctttgtt
     3301 ccaggcggag cgtttgctgc tggatggact gcgtatcctc ctctcagtgc gggcacatac
     3361 gctggttcaa aagacttctt gagcaacatg ggcggtcacc tctggatcat tgcgatcgcc
     3421 cttgagttca tcgcattcct catgggtggt atcaacttcc tggttacttc cttcaacatg
     3481 agagccccgg gcctgagctg gtttcgcctt cctgtagtag tgtggatgct ggatcttgcc
     3541 gttctcctct tcatgttctc gattggacca ctcctcgctg gcgccatcat gcttctcttt
     3601 gatcgcacgg taggtactgg gttctatgac ccggctcagg gtggagaccc gattctgttc
     3661 cagcatttgt tctggttctt cggtcatcct gaagtgtacg taatgcttct tcccactctt
     3721 ggattcctgt gcgagattct ggcaactttc gcgagaaagc caatcttcgg ttacaagtac
     3781 atcctctatt tcacctttgg tgcggctctt ctgtccttca tcgtatgggc acaccaccag
     3841 tttgtatccg gaatcaatcc tcgcatggct aacttcttta gcttaacgac tctgctgatt
     3901 tctgttccat ttactgtcat cgtgttcgca agtattgcga ctctgtggcg cggcaacatt
     3961 cagttctcag tgcctatgct ctgggctgtt ggtttcgtgg cagagttcat gattggcggt
     4021 gtgaccggtg tataccttgg ttcttccgcg tttgatatct acgctcatga tacgtacttc
     4081 gtgatcgcgc atttccacta tgctctcatc ccaacggttg tgtttggagg gtttgccgcc
     4141 at
//
LOCUS       DAORFF010000352         4141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12859_length_4141_cov_3.273128, whole genome shotgun sequence.
ACCESSION   DAORFF010000352 DAORFF010000000
VERSION     DAORFF010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12859_length_4141_cov_3.273128"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PKK76_13315"
     CDS             complement(<1..264)
                     /locus_tag="PKK76_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL69505.1"
                     /translation="MKTKITTKGEVTIVKIEGAIKSGDEYELAEKMEKYIKPDSAPKF
                     IVDMKKVPFINSAALGLFLNIFKHVDYLKGRMVFAGLNSDVENL"
     gene            complement(215..781)
                     /locus_tag="PKK76_13320"
     CDS             complement(215..781)
                     /locus_tag="PKK76_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69506.1"
                     /translation="MNLQKNASSLSNPMDILEALLPAGRMPASMLRSDQNTQEMQEIL
                     ADMAACDLARVPARPHFFKANKNGLVSSFGLRPSLAFRSKSRIVHSDIIALDKDYLIV
                     ELSADETGLHIVMEKGAKNESTISISLYRGAAAVEFLHIKSRVRKDLGELAPGEYSLI
                     CENRPVLNFSLFDENENHNKRRSNNRKD"
     gene            complement(778..1536)
                     /locus_tag="PKK76_13325"
     CDS             complement(778..1536)
                     /locus_tag="PKK76_13325"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HNL69507.1"
                     /translation="MLENDPLQNLPGDYYRTEDLREFMNASASWMMRYIQIRLTRDPD
                     RAGDFYLHFYERAARCLEAYRSRQHLPFTGFLATYLRHEFMNFLRTAKTREGSEVKAD
                     DMSYFPAADERPPSPESDVPADLPLNCRIPLKLHSGLHLNSEEIRSLAVELGPGATAS
                     LLKEAMQRRMSRDRRTQKLEDRAARLNYLIHTSKAPASRLKKWKNRIVAMLDRPRPIF
                     SYSELAERLGTSKTTISRRIDQARTGVRVIQEAL"
     gene            1650..2507
                     /gene="dapF"
                     /locus_tag="PKK76_13330"
     CDS             1650..2507
                     /gene="dapF"
                     /locus_tag="PKK76_13330"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNL69508.1"
                     /translation="MVHLPFFKMEGAGNDYVYLDALEDPALVLKLTPEMIARMSDRHF
                     GIGGDGVVMILPSGDSEALMRMWNADGSESSMCGNALRCVAFYVHKKNGKRNFTIETK
                     SGRHAALIVESSAKHGTVQVQIGQPRTGPDEIPVNPDVLGLTGKGPFLDVPVSVSGFE
                     GKGTLVSMGNPHCVFFVEDPDSLDLEKIGPLLEHHPAFPARTNVEFVCVQSDGTIRQR
                     TWERGSGETLACGSGVCAVLVASVLSRNMPRKNTIHVRGGDLEASWTEDGVMLSGPAR
                     IVFEGTFSV"
     gene            2504..2695
                     /locus_tag="PKK76_13335"
     CDS             2504..2695
                     /locus_tag="PKK76_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNL69509.1"
                     /translation="MSDTNARLSAQDVESPCNDICIMDEYTGYCRGCLRTIDEIARWA
                     SYSPEQKREVLLKIKERAE"
     gene            2692..3918
                     /locus_tag="PKK76_13340"
     CDS             2692..3918
                     /locus_tag="PKK76_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNL69510.1"
                     /translation="MRQYWKRFLVFFSFVTTVLGIAYSFVAVRLTVGLVHPGLIALVW
                     ILAAVSLLLAPLAFALRMFQVPERIEKIIAWTSYLSFGFFAMLFALIASRDLGLVAVD
                     QVGGIVDRFLPWRDPANLLHPMHREFLVATSNAALLGLAGGMFVYGVIRARAIGIERV
                     QVPIDGLPESLHGFTIAQITDIHIGPTLKRSFALEIVKKVNNLKADMIAVTGDVVDGS
                     VQQLRDHTEPFADLEARHGVFFVTGNHEYYSGALSWISEFRNLGFQVLLNEHSVLEHA
                     PGGNVMVAGVTDYRAAEFFDDHRSDPALALRRAARGSRSSKEPHVKILLAHQPRSIAG
                     AADAGFDLQISGHTHGGQFFPGPYLVRLQQPYTKGLHRHGDKTWIYISRGTGYWGPPI
                     RVGASPEITLIELVRR"
     gene            complement(3982..4137)
                     /locus_tag="PKK76_13345"
     CDS             complement(3982..4137)
                     /locus_tag="PKK76_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69511.1"
                     /translation="MGGGVSQIPELYTIGVERIKKYTFYKNPKTPVLQNKLGDSAGVF
                     GAAWIGV"
BASE COUNT          936 a         1002 c         1107 g         1096 t
ORIGIN      
        1 caggttttcg acatctgaat tcaagcccgc aaacaccatt ctacctttca gataatctac
       61 gtgcttgaat atattcagga acaagccgag cgccgcagaa ttaatgaagg gcactttttt
      121 catgtccaca atgaattttg gagcgctgtc tggcttgatg tacttttcca ttttttccgc
      181 cagctcgtac tcatcgcccg atttaatcgc gccctcaatc tttacgattg ttacttcgcc
      241 ttttgttgtg attttcgttt tcatcaaata aactaaaatt cagtactggc ctattctcgc
      301 agatcaggga gtattctccc ggtgcaagct cacccaggtc tttccgaacc ctgcttttta
      361 tgtgcaagaa ttctaccgct gcggcgcctc tgtaaagtga aattgatata gtgctctcgt
      421 tttttgcgcc tttctccatc acgatgtgca aaccagtctc atctgcgctc agttcaacga
      481 tcaaatagtc cttgtccagg gctatgatgt cgctgtgaac gatgcgtgac ttcgaacgga
      541 aggccaggga cgggcgaagc ccgaaggagg ataccagacc attcttgttc gctttgaaaa
      601 agtggggccg ggctggcact ctggcaagat cacaggctgc catatctgcg aggatttcct
      661 gcatttcttg cgtgttctga tccgagcgaa gcatgctcgc gggcattctg cccgccggaa
      721 gcagggcctc gaggatgtcc atggggtttg acagagagct tgcattcttt tgaagattca
      781 tagtgcctcc tggatcaccc ggactcctgt tcgggcctgg tcgattcttc tggatatagt
      841 agttttactg gtaccgagcc tttctgcgag ctctgagtaa gaaaaaatag gacgcggtcg
      901 atcgagcatt gccacgattc gattcttcca cttcttcaag cgggacgccg gtgctttcga
      961 cgtatgtatc aagtaattta gccgggctgc tcggtcctcc agcttctgag tgcggcgatc
     1021 gcgagacatt cttctttgca tggcctcttt gagaagggac gctgttgcac ccggacccag
     1081 ttctaccgcc aggcttctga tttcttctga gttcagatga agtccggaat gaagtttcag
     1141 cgggattcta cagttcagcg gaaggtccgc gggaacatcc gattcaggcg acggcggtct
     1201 ttcatccgcc gcaggaaagt aagacatgtc atccgctttg acttcgctcc cttctcttgt
     1261 tttggcggtt ctcagaaaat tcatgaattc gtggcgcagg taggtagcaa gaaacccggt
     1321 aaacggcaga tgctgcctgg acctgtaagc ttcgaggcag cgggccgccc tttcgtaaaa
     1381 atgaagatag aaatctcctg cacggtccgg atcgcgagtg aggcgaatct ggatgtagcg
     1441 catcatccag gaagcgctgg cgttcatgaa ttccctgaga tcttctgtgc gatagtagtc
     1501 cccgggcagg ttctgtagcg gatcattttc tagcatggtc gttcaaaaaa agaagggtgg
     1561 agcaatggaa aatcgttccg cgctttttcg atcaaaaaac aaatccggga aaaaaaagtg
     1621 cccgcccagg ccctggcgct ttttgtgtaa tggtgcacct tcctttcttt aaaatggagg
     1681 gcgccgggaa tgattatgtc tacctggacg ccctcgaaga cccggctctg gttttgaaac
     1741 tcactccgga gatgattgcg cgcatgagcg atcgacattt tggaatcggc ggagacggtg
     1801 tagtgatgat tcttccttcc ggtgactcgg aagcactcat gcgcatgtgg aatgcagatg
     1861 gatcagaatc ttccatgtgt ggaaatgctc tcagatgcgt tgctttctat gttcacaaaa
     1921 aaaatgggaa gcggaacttt acaatagaaa cgaaaagtgg acgtcatgcg gcattgatcg
     1981 tcgagagctc cgcaaagcat ggaacagtcc aggtgcagat cggtcagcct cgcaccgggc
     2041 cagacgaaat cccggtaaat ccggatgtgc tcggacttac cgggaaagga cctttcctgg
     2101 acgtgcctgt ttctgtgtct ggatttgaag ggaagggaac tttagtttct atgggcaatc
     2161 ctcattgtgt tttctttgtg gaggatccgg acagtctcga cctcgaaaag attggaccac
     2221 ttctagaaca ccatcctgct tttccggcgc gaaccaacgt tgaatttgtc tgcgtgcaat
     2281 cagacggcac gattcgccag agaacgtggg aaagaggaag cggggaaacc ctggcgtgtg
     2341 gcagtggcgt ctgcgctgtg ctcgtcgcca gcgttctatc tcgcaacatg cctcggaaaa
     2401 atacaattca tgttcgaggc ggggatctag aagcaagctg gacggaagat ggtgtgatgc
     2461 tctccggtcc ggcccgcatt gtgtttgagg gaactttctc agtatgagtg atacgaatgc
     2521 tcgcctgtcg gcgcaggatg tggaatctcc gtgcaatgat atatgcatca tggatgaata
     2581 tactggatac tgcagaggct gcttgcgcac catagacgag atcgcacgct gggcttccta
     2641 ttcgcctgaa cagaaaagag aagttcttct gaaaatcaaa gagagggcag agtgagacag
     2701 tactggaaaa gatttctcgt attctttagt tttgtaacta ctgttcttgg aattgcctat
     2761 tcttttgtgg cagtgcgcct gacagtcggg ctggttcatc cggggctcat agcgctggtc
     2821 tggattctag cggcagtctc tctgcttctg gctcctcttg cgtttgctct tcgcatgttc
     2881 caggtccccg agagaatcga gaagatcatc gcgtggacta gttacctttc ctttggtttt
     2941 tttgccatgc tctttgctct gattgcttcg agagatctgg gtcttgtggc tgtagaccag
     3001 gtgggtggaa ttgtggatcg atttttaccg tggagagatc ccgcaaatct gcttcatcca
     3061 atgcatcgtg aatttctcgt ggccacaagc aatgcggcac tgttgggtct ggccggtggc
     3121 atgttcgtgt atggtgtgat tcgggcgcgg gccattggaa ttgaaagagt ccaggtaccc
     3181 atcgacggcc tgccagagtc tttgcacggt tttaccattg cgcagatcac ggacattcac
     3241 ataggcccaa cactgaagag aagcttcgca ctcgaaatag taaagaaagt aaacaatctg
     3301 aaagcggata tgattgcagt aaccggagac gtggtggacg gaagcgttca acagttgcgc
     3361 gatcacaccg aaccttttgc ggatctcgaa gcaaggcacg gagtgttttt tgtaactggc
     3421 aatcacgagt attattctgg agcgctttcc tggatttcag aattcagaaa cctgggtttc
     3481 caggttttgc tcaatgaaca ctctgtgttg gagcatgcgc cgggcggaaa tgtaatggtg
     3541 gccggagtca cggattacag ggccgccgag tttttcgatg atcacagatc agatcctgct
     3601 cttgctcttc gccgcgctgc ccgtggaagc cgatcttcca aagaacctca tgtgaaaatt
     3661 ctactcgcac atcagcctcg tagcattgcg ggggcggcag acgcaggatt tgatcttcag
     3721 atatccggcc acactcacgg cggtcagttt tttccagggc cctatctggt gcgattgcag
     3781 caaccataca cgaaaggatt gcatcgtcat ggagataaga cctggattta catcagccgc
     3841 ggaactggtt actggggccc gccgattcga gtgggtgctt ctccagagat cactctgatt
     3901 gagcttgttc gtcgatagtg ctcagccggt gcgtcgttca tgcgtcgcta gcgcgcggat
     3961 ggcctgcagg ccgcagagca atcaaactcc aatccaggcg gctccaaaca ctccggctga
     4021 atcaccaagc ttgttttgca atacaggcgt ctttggattc ttataaaaag tatacttttt
     4081 gattctttcc acacctatgg tgtagagttc tggaatctga gatacaccgc cgcccagaac
     4141 g
//
LOCUS       DAORFF010000353         4135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12895_length_4135_cov_2.886029, whole genome shotgun sequence.
ACCESSION   DAORFF010000353 DAORFF010000000
VERSION     DAORFF010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12895_length_4135_cov_2.886029"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..482
                     /gene="acpP"
                     /locus_tag="PKK76_13350"
     CDS             246..482
                     /gene="acpP"
                     /locus_tag="PKK76_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNL69512.1"
                     /translation="MADMEKIKAIIVEQLGVDESEVTPEAHFIDDLGADSLDTVELVM
                     ALEEEFGIEISDEDAEKIQTVGDVSRYIDEHASK"
     gene            527..1276
                     /gene="rnc"
                     /locus_tag="PKK76_13355"
     CDS             527..1276
                     /gene="rnc"
                     /locus_tag="PKK76_13355"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNL69513.1"
                     /translation="MGIRLFRKTQDLPPDRKARLAVFAKKTHLPFKDLTLLHGALVHS
                     SFAHEQKPPIPDNQRLEYLGDSVLGLIINDSLFRSYQNMDEGRMARIKASLASEQTLA
                     RAASRISLGEVLLLGRGEKQTGGKTRSSILADALEAVIGALYVDRGWQAVYTFVTELL
                     ADEIEKLSGREDVRDPKTKLQEYLQKQGKALPEYKLLGKIGPDHKSTFQISVSLSGKE
                     IAQGTGESRKKAEHQAASNALKKFHRMRIGN"
     gene            1290..2372
                     /gene="aroB"
                     /locus_tag="PKK76_13360"
     CDS             1290..2372
                     /gene="aroB"
                     /locus_tag="PKK76_13360"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNL69514.1"
                     /translation="MEAAANIRLPGSAPYEYPIFIQESYAGLAQILRERPGKLFVISQ
                     KGLESVLDLFFRESGVPRDRLYLIEQGEENKHLNQLGEVYNWLIQSGIDRSSSIIAPG
                     GGVVGDFAGFVASTILRGVPFIQIPTTLLASVDSSVGGKVAVNADFGKNMVGSFYHPA
                     FVYCNVSVFSTLPEDEWSCGFAEIVKHSFLEETSRDLNYLEQNVQNIRKPEILSLVIR
                     DNVRFKASIVSQDEKEAGLRSILNLGHTTAHALESITHYKKFSHGQAVSRGLVTALLL
                     SEKTGLSATDRERMLSLMQKLALPLDTAGFGAAELLEHMKFDKKNTGGQIRFVLLEKP
                     GKPVFGIPITAEEFENAWSAQKRLFG"
     gene            2342..3499
                     /locus_tag="PKK76_13365"
     CDS             2342..3499
                     /locus_tag="PKK76_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69515.1"
                     /translation="MVRTKTVVWLMSAREEIASLMLEGRFGEAYRKCDQALRGDSGGG
                     QKSKVASDADFFRIAGAVSLLGLGHIRQAEEWTANVESTDPAFYYIEALIHLHAGRTH
                     EALLCYTRLIDEDPSDTFADRLIEKLRAHPDEIVSEVQETAIGRYLPIKHWFGEDGLI
                     KRKTEEKPVSPPLDRLRARFKSIRFTAKPVRWVMAGSIAFLFLVAAIFLYLKFGNRDH
                     DLAERLPDPPTQGTIIPLSELKKDEVRFTFDSRKEVIDQYELARRKVIDARVNEARVL
                     LNEIDLSNAGFELKERALLLKQMIPYIAPSRFSDNVEIQKVVEDPDLYQGVQVLWPGE
                     IVNEPTGPVLRTAGGKVRLNGSVPSSGTVKVSGFFQRIDRDPVVDVREIVR"
     gene            3556..3723
                     /locus_tag="PKK76_13370"
     CDS             3556..3723
                     /locus_tag="PKK76_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69516.1"
                     /translation="MTSAVAQPINPFHKAPPAEPAAQLFSRQEMHRYLDRMNPIEVPG
                     EDRGKNLDLQA"
     gene            3727..>4135
                     /locus_tag="PKK76_13375"
     CDS             3727..>4135
                     /locus_tag="PKK76_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNL69517.1"
                     /translation="MNRTEFGPDFVFGTATAAYQIEGAWQEDGKGESIWDRFSHTRGK
                     IENNQTGDVACDHYHRFESDLDILKSLGIQHYRFSISWSRIFPEGRGRVEQRGVDFYK
                     RLIAACAARGITPWVTLFHWDLPQALEDKGGWPA"
BASE COUNT         1031 a         1039 c         1072 g          993 t
ORIGIN      
        1 ggagtcgcat ttctatcctc tgatatggcg tctttcatca caggccacgt actggagatc
       61 aacgggggcg gttttcagcc agcttctgca gactaatcac agtttttgca cgtgcgaaga
      121 atccgtggtt ttttcaagac cacggatttt tttttggttt tgcacttgcc cgcgcaaagg
      181 gcatggtcat tttgagcacc tcgtacctac tacaggggta ccaatcaaaa acgtggaggc
      241 ctataatggc tgatatggaa aaaatcaaag ctatcatcgt cgagcagctt ggagtagacg
      301 aatcagaagt cactccagaa gctcacttca ttgacgatct cggcgctgac tcgctcgaca
      361 ctgttgagct tgttatggct cttgaagaag agttcggcat cgaaatctct gacgaagatg
      421 ctgaaaaaat tcagacagtt ggcgacgtat ctcgttacat cgacgagcac gcttccaagt
      481 aatctgctgc gccggcaatc gcaagattgc cggcactttt ctctgaatgg gcattcggct
      541 gtttcgtaag acgcaagatc tgccccctga ccgcaaagcc agactggctg tattcgcgaa
      601 aaagactcac ctacctttca aagacctgac cctccttcac ggcgcgcttg tgcactccag
      661 cttcgcgcat gaacaaaaac ctcccatacc ggataaccag cgtctggaat atctgggaga
      721 ctccgtgctt ggcctcatca taaacgattc actatttcgc agttatcaga acatggacga
      781 aggaagaatg gcacgcatca aggcaagcct cgcgtccgaa caaactctcg cgcgtgctgc
      841 ttcgcgcatt tctctcggag aagtattgct gcttggccgt ggagaaaagc agacaggcgg
      901 aaagacccgc tcatcgattc tggcagatgc tctcgaagct gtgatcggtg cattgtatgt
      961 ggatcgcggc tggcaagctg tctatacttt tgtgacagaa ctgcttgcag acgaaataga
     1021 gaaactctcc ggacgcgagg atgtccggga tcccaaaacc aagcttcagg aatacctgca
     1081 gaaacagggc aaagcactgc ccgaatacaa actgcttgga aaaataggac cagatcacaa
     1141 gagcactttt caaatttcag tttctctttc tgggaaagaa attgcccagg gcacgggtga
     1201 atcgcgcaaa aaggcagaac accaggcagc cagcaatgcc ctaaagaagt ttcaccgcat
     1261 gcggattgga aattgatggt aaacagatga tggaagcggc ggcaaacatc agacttccgg
     1321 gaagtgctcc ctacgagtat cccattttca ttcaggaatc gtacgccggt ctcgcccaga
     1381 tcttgcgcga aagaccaggc aaactttttg tcatcagcca gaaaggtctt gaatcggtcc
     1441 tggatttgtt tttccgcgaa tcgggtgtgc ctcgcgatcg cctctatttg atagagcagg
     1501 gagaagagaa caaacacttg aaccagctcg gtgaagtcta caactggttg atccagtcag
     1561 gaatcgatcg ctccagttct atcatcgccc ctggcggcgg cgtcgttggc gatttcgcag
     1621 gatttgtcgc atccacaatt cttcgcggag ttcctttcat tcagattcca accactctgc
     1681 ttgcttctgt ggattcgagc gtgggcggga aagtggcagt gaatgccgat ttcggaaaga
     1741 atatggttgg ttctttttat catccggcct ttgtatactg caatgtttct gttttctcca
     1801 ctttgccaga agacgaatgg tcctgcggct tcgcagaaat tgtgaagcat tcttttctgg
     1861 aagaaacgtc gcgcgacctg aactatctgg aacagaacgt gcaaaatatt cgcaaacccg
     1921 agattctctc tcttgtgatt cgcgacaatg ttagattcaa agcgtccata gtttcgcagg
     1981 atgaaaaaga agcgggcctg agatccatcc tgaacctggg tcacaccaca gcacatgcgc
     2041 tggagtctat cactcactat aaaaagtttt cgcacggcca ggctgtgtcg cgtggcctgg
     2101 tcacagcact tctactttca gagaaaacgg ggttgtctgc tacggacaga gagcgcatgc
     2161 tttccctgat gcagaagctt gctcttcctc tggacactgc tggattcggg gcggccgagc
     2221 tgcttgagca tatgaaattc gacaaaaaga acacaggcgg gcagattcgt tttgttcttc
     2281 ttgaaaaacc aggaaagcca gtattcggga tcccaatcac agcggaagaa tttgaaaacg
     2341 cgtggtccgc acaaaaacgg ttgtttggct gatgagtgcg cgcgaagaaa tcgcatcctt
     2401 gatgctcgaa ggcagattcg gcgaagcgta tcgcaagtgt gatcaggctc tcaggggcga
     2461 ttcgggggga ggccagaagt ccaaagttgc gtcggatgct gacttctttc gcattgcagg
     2521 cgctgtctcc ttgctgggcc ttggacatat ccgccaggca gaggagtgga ccgccaatgt
     2581 cgagtctacg gatcctgcgt tctactatat agaggctctc attcatttgc atgcaggtag
     2641 aacccacgaa gctttgctct gctacactcg cctcatagac gaagacccgt cagatacatt
     2701 cgcagacaga ttgatcgaaa agcttcgcgc ccatccagac gaaattgtga gcgaagttca
     2761 ggaaacggcc attggacgtt atctgcccat caaacactgg tttggcgaag atgggctcat
     2821 caagagaaaa acagaagaga aacctgtttc gccaccgctc gatcggcttc gtgctcggtt
     2881 taaatcgatt cgattcacag caaaaccggt gagatgggtg atggcagggt ccattgcttt
     2941 tctgttcctg gtcgctgcta tctttttgta cctcaagttt ggcaacagag atcatgatct
     3001 cgcagagaga cttcccgatc cacccaccca gggcactatc attccacttt cagaactgaa
     3061 gaaggatgaa gttcgcttta cgtttgattc gcgcaaagag gtcatcgatc agtatgagct
     3121 tgcgcgcagg aaggtcatcg acgcacgcgt gaacgaagcc cgagtgttgc tcaatgaaat
     3181 tgacttatcc aatgcaggct ttgaactcaa agagcgggcc cttctgctca aacagatgat
     3241 cccttacatc gccccttcgc gtttttcgga caatgttgaa attcagaaag tagtagagga
     3301 tccggacctg taccagggag tccaggttct gtggccagga gagatcgtaa atgagcccac
     3361 aggacccgtt cttcgcacag ccggtggcaa ggttcgcctg aatggctccg ttccgtcttc
     3421 gggaacagtc aaggtcagcg gatttttcca gagaatcgac cgagatccag tcgtggatgt
     3481 tcgagaaatc gttcgctgaa tttttcttgc agtcttcccg cgcgggagct attttagcag
     3541 tagatggaag ctcaggtgac atcggctgta gcacagccaa tcaatccgtt ccacaaggcg
     3601 cctccagcgg agccggcggc ccaattgttc agcaggcagg agatgcaccg ctacctggat
     3661 cgcatgaacc ccatcgaagt ccccggtgaa gaccgtggca agaaccttga cctccaggct
     3721 tgatgagtga atcgaactga atttggcccc gatttcgttt ttggaactgc aacggcagcc
     3781 taccagattg agggagcgtg gcaagaagac ggaaaaggtg aatcgatctg ggatcggttc
     3841 agtcacactc gtggcaaaat cgaaaacaac cagaccgggg atgttgcctg cgatcactac
     3901 catcgctttg agtctgatct tgatatatta aaatccctgg gaattcagca ctatcgcttt
     3961 tctatttcct ggtctcgcat ttttccggaa ggtcgcggta gggtagagca gcgcggcgtt
     4021 gatttctaca aacgattgat tgctgcctgc gccgcgcgcg ggatcacacc ctgggtgacc
     4081 ttgtttcact gggatttacc acaggcgctc gaagacaagg gtggatggcc cgctc
//
LOCUS       DAORFF010000354         4119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_12966_length_4119_cov_3.150098, whole genome shotgun sequence.
ACCESSION   DAORFF010000354 DAORFF010000000
VERSION     DAORFF010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12966_length_4119_cov_3.150098"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKK76_13380"
     CDS             <1..371
                     /locus_tag="PKK76_13380"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNL69518.1"
                     /translation="LTPHGGEWKKMGGQLPDCVQAFEESQAFARTLGVSVLVKGPVSV
                     LFSAKTWIHSHPNPSLAVAGSGDCLGGIILSSLSRGHEMAAGVLSSMDLLHEAVSGLT
                     HPEAHEFAENIRRILSVTRA"
     gene            complement(415..1140)
                     /locus_tag="PKK76_13385"
     CDS             complement(415..1140)
                     /locus_tag="PKK76_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69519.1"
                     /translation="MTEAKRQLLPDENWFWKIVYAVSRTGFFIFVSACLPGAYLYIRG
                     QSAPVWLALPVAIGGCIWTLIYLGLHSYAFPTHGRGALLAFVVPVWNCVASGQATESF
                     LFGAYSVVLAAVVAQCLFLLPVSLAACISPLRRRIFGEDSALWIAPFLSVVFLLIAAF
                     GWTFRAPFLEQMQSTRSFVAMTGFAFQVVMEIRVLYFGSIFSSSRGEDSESSALHEAW
                     APLMIFCMIGSLAGAGILALWRS"
     gene            complement(1165..2790)
                     /locus_tag="PKK76_13390"
     CDS             complement(1165..2790)
                     /locus_tag="PKK76_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNL69520.1"
                     /translation="MKGLMMDYNLTVDRILLRAEKMFPEQEIVSRLANGSIHRYKYGE
                     FFSRTIKLMNALRALGVKPGDRVATFAWNSYRHMEIYFAVPALGAVLHTLNIRLFPEQ
                     LVYIVNHAKDKLIFVDKSLSGMLAAQEPHFKGVQNYIVMDDLHAEPAASLPNTLDYEA
                     ILSAGKDTIEFPTIEEGTAAGMCYTSGTTGEPKGVLYSHRSIFLHAMGISMTDSLAIS
                     NRDVILPVVPMFHVMSWGIPFACCLTGAKPVYPGAQLIGKPIAELLEKERVTIAAGVP
                     TIWNVLLQHLQKETHDLSSLKTMVVGGSAAPRSMIEAYEKKFGVSILHAWGMTETSPL
                     GTVSRLKKHMDTWSEDEKYKVRAKQGMAVPLVEVKIVDDENKDLPWDGSSSGELLVRG
                     PWIASSYYNAETPDGNAAFTKDGWFRTGDVATIDSNGFMEITDRKKDLIKTRGEWISS
                     VEMENIIMSYAGVLEAAVVGRPDEVRGEAPVAFVVATAEKKGTLDAKGIHDLLSEKFA
                     HWQIPKLSDIRFIDAVPKTSVGKFDKKVLRKQV"
     gene            complement(2836..3147)
                     /locus_tag="PKK76_13395"
     CDS             complement(2836..3147)
                     /locus_tag="PKK76_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69521.1"
                     /translation="MGFTPRQFKLVQAAAGSLESLGIAREEAIILISNSIQEELTALG
                     LTMEQVDAKSRTERFAFIRTLAARMEKTIHTRGVMLGQIRAAMEEFMEALRQSWQEET
                     R"
     gene            complement(3198..3707)
                     /locus_tag="PKK76_13400"
     CDS             complement(3198..3707)
                     /locus_tag="PKK76_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNL69522.1"
                     /translation="MPVELRALEISMKSDLAKLANDPEIFVNVRDFFPHPYSELNAEE
                     FISRSSLNPAPLEYAILTDGAFAGVCGFIPGTDIHRLTAEVGYWIGKPFRGKGIATSA
                     LRQLCDVAFSRGFVRLYAGVFETNQPSMRVLEKSGFTLESIRKKGVIKQGKILDEHLF
                     VLLRPDTSL"
     gene            3828..>4119
                     /locus_tag="PKK76_13405"
     CDS             3828..>4119
                     /locus_tag="PKK76_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HNL69523.1"
                     /translation="MAWHVVIGLEVHVQLGTRTKIFARDGYGFGSPANSLAGPVTLGL
                     PGVLPVLNEEVLRLAVIAGLALECKIAEISKFDRKHYFYPDLPKGYQISQYDQ"
BASE COUNT         1016 a         1108 c          997 g          998 t
ORIGIN      
        1 tgcttactcc acacggtggt gaatggaaaa agatgggcgg tcagcttcca gattgtgtcc
       61 aggcctttga agaatcgcaa gctttcgcgc gaacgctcgg ggtttctgtg ctcgtcaaag
      121 gcccggtcag tgtcttgttt tccgccaaaa catggattca cagtcacccc aatccttctc
      181 ttgctgttgc gggtagcggg gattgcctcg gagggatcat tctttcttct ctatcgaggg
      241 gccatgaaat ggctgcgggc gttctttcct ctatggatct gttgcacgag gcggtttctg
      301 gtctcactca tccagaggcg cacgaattcg cagaaaacat tcgtcgcatt ctatctgtta
      361 cgcgtgcgta gcattcgcgt tcacagttgc gcacccatga gttgcggccg cccttcacga
      421 tctccagaga gccagtatgc ccgcacctgc cagagatcca atcatgcaaa aaatcataag
      481 cggcgcccac gcttcatgca gtgcggacga ttctgagtct tctcctctcg aagaagaaaa
      541 aatactgccg aagtacagca ctcgaatctc catcactacc tggaaagcaa agccagtcat
      601 ggctacgaaa ctgcgcgttg attgcatctg ctccaaaaac ggagcacgaa atgtccaacc
      661 aaacgcagcg atcagtagaa agactacaga caaaaacggc gcgatccaga gggcgctatc
      721 ttctccaaaa atacgacggc gaagaggcga aatgcaggca gccagggaaa caggaagcag
      781 gaacaaacac tgcgctacaa ctgcagccaa cactacagag taggcgccaa atagaaacga
      841 ttctgtggcc tgaccagacg caacacagtt ccatactgga actacaaacg caagaagtgc
      901 ccctcggcca tgagtgggaa atgcgtagct gtgcagccca agataaatga gagtccagat
      961 acaaccacca atggcgactg gaagagcaag ccagacgggt gcagattggc cgcgaatgta
     1021 tagataagcc ccgggaagac aggcggatac gaatataaaa aaaccagtcc gactgactgc
     1081 gtatacaatc ttccagaacc agttttcatc cggaagcaac tgtctttttg cttctgtcac
     1141 tgtatcatcc agttcgcgcg cgcgtcacac ttgctttcgc aacactttct tatcgaactt
     1201 cccaacgctg gtctttggaa ccgcatcaat gaaacgaatg tcgcttaact ttggaatctg
     1261 ccagtgcgcg aacttttcgc tcaatagatc atgaatgcct ttcgcatcga gagtgccttt
     1321 cttttccgcc gtagccacaa cgaaagcaac cggagcctcc cctctgacct cgtctggtct
     1381 gcctacaacg gcagcctcaa gcactcctgc atacgacatg atgatgtttt ccatttcaac
     1441 gctcgaaatc cactctcccc tggtcttgat gaggtctttc ttacgatccg tgatttccat
     1501 aaatccgttg gaatcaatgg tggccacgtc tcccgtgcga aaccatccat ccttcgtgaa
     1561 cgcggcgttt ccatccggag tctctgcatt gtaataggaa gaagcaatcc agggtccgcg
     1621 caccaggagc tcgccagaac tcgagccgtc ccaggggaga tctttgtttt catcgtctac
     1681 aattttcact tccacgagag gaacggccat cccctgcttg gcgcgaacct tgtatttctc
     1741 gtcttcgctc cacgtgtcca tgtgcttctt cagtctgctc actgttccaa gcggagacgt
     1801 ctctgtcata ccccacgcat gtagaataga aactccaaac ttcttctcat atgcttcaat
     1861 catggaacgc ggcgcggctg atccgcccac caccatggtc ttaagcgaag acagatcatg
     1921 tgtctctttc tgcaaatgtt gaagcaatac attccagatt gtgggaactc cggcggcaat
     1981 ggtaacacgt tctttttcca gtagctctgc aattggcttt ccgatcagct gcgctcctgg
     2041 atacactggc tttgcgcctg tgaggcaaca ggcgaaaggg atgccccagc tcatcacatg
     2101 aaacatgggc actacaggca gaatcacatc tcgattggaa atcgcaagag agtccgtcat
     2161 gctgattccc attgcatgca gaaaaataga acgatgactg tagaggactc ctttgggctc
     2221 tcctgtggtg ccggaagtgt aacacattcc cgcggccgtg ccttcttcta tagttggaaa
     2281 ttctatagta tccttacccg cacttaaaat ggcctcgtag tccagagtgt tcggaagcga
     2341 agcggccggt tccgcatgga gatcgtccat gacaatgtag ttctgaactc ctttgaagtg
     2401 aggttcctgc gctgccagca ttccagagag agatttatcc acgaagatga gcttgtcttt
     2461 ggcatggttg acaatgtata caagctgctc cggaaaaaga cgaatgttca gagtgtgaag
     2521 gacagctccc agggccggca ctgcaaaata gatttccatg tggcgataac tgttccatgc
     2581 gaaagtggcc acgcgatctc ctggctttac tcccagagcg cgaagagcat tcatcagttt
     2641 tatggtgcgt gagaagaatt cgccatattt gtatctgtga atggatccat ttgccaggcg
     2701 acttacgatt tcttgttccg ggaacatctt ctctgctcgc aggagaatcc tgtccacagt
     2761 gagattgtag tccatcatga gccctttcat gggctgagtt tccgacctcg ggcgacgggg
     2821 gtcaatccac aattgtcagc gcgtttcttc ctgccaggat tgcctgagcg cttccatgaa
     2881 ttcttccatg gctgcacgga tttgtccgag catcactccg cgcgtgtgga ttgtcttttc
     2941 cattctcgcc gcaagtgtac ggatgaatgc aaatctttcc gttctgcttt tcgcatccac
     3001 ctgctccatg gtcagaccaa gtgcggtgag ctcttcttga atggaattgg aaatcagaat
     3061 gatggcctct tcgcgcgcaa ttcccagact ttccagactg cctgccgccg cctgaacgag
     3121 tttgaattgt cttggagtga accccatgaa gtgatcatcg actgctcaga gcagggatca
     3181 ataaaaaaat gcaacgatca aagagacgta tcagggcgca gtagaacgaa caggtgctcg
     3241 tccaggatct ttccctgctt gatgacgccc ttctttcgaa tcgattcaag agtaaaacct
     3301 gacttttcga gcactcgcat ggatggctga ttggtttcaa atactcctgc atacaagcgg
     3361 acaaagcctc gggaaaatgc tacatcgcaa agctgtcgaa gcgcagaagt tgcgatcccc
     3421 ttcccgcgaa atggcttccc gatccagtac cccacttccg cggtcagacg gtggatgtct
     3481 gtgcctggaa tgaaaccgca tactccggca aacgcaccgt ctgtgaggat cgcatactcc
     3541 agaggtgcgg gattcaggga agacctcgaa ataaattcct ccgcgtttag ttcgctgtac
     3601 ggatgcggaa agaaatcccg tacatttaca aatatctcgg gatcattggc cagctttgcc
     3661 agatccgact tcatggaaat ttctagagcg cgcaactcta caggcatgtg ccagatcgct
     3721 cgggggagaa aaaaagcgca catacttttg cagacgccgt caggctaatc ggcacaaaat
     3781 acccagacaa ttacttgcgc cccaaagtcg tcgggctcag ttgactcatg gcctggcatg
     3841 tagtcatcgg attggaagtt cacgttcaac tgggaactcg cactaagatt ttcgcgcgtg
     3901 acggatacgg atttggttcg cctgcaaact ctctggcggg accggtcaca cttggattgc
     3961 ccggagttct acccgttctc aatgaggaag tgcttcgcct cgcggtcatt gctggcctcg
     4021 cactcgagtg caagattgca gaaatttcca agtttgatcg caagcattac ttttatccag
     4081 atcttccaaa aggttaccag atatctcagt acgatcagc
//
LOCUS       DAORFF010000355         4111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13006_length_4111_cov_3.573225, whole genome shotgun sequence.
ACCESSION   DAORFF010000355 DAORFF010000000
VERSION     DAORFF010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13006_length_4111_cov_3.573225"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1188)
                     /locus_tag="PKK76_13410"
     CDS             complement(91..1188)
                     /locus_tag="PKK76_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNL69524.1"
                     /translation="MYDFRLLFVTWVHYDGIMRNILAAFGFLIRLSLKQRKSYSSEYI
                     TINDESIGVDIYDPIGPSKGCILTINGFAPLGNRDERFLHLNSALAGIGFTVVSPHLP
                     GFCNLQIDSAHVSLIQRLTLAISHRRDLTRSGKVAVLAPSFAGGMTLIAAATASSEHI
                     SAICTIGTYANVDALLDSLMLNQNADEYGRLIILRNFLHLSAGRQPRIATAITHILHD
                     SYFHARDPVFPDYEKTLRRTERTFLLRLREDSNFRMRHWKRIIKKSGKRRSILAQICA
                     MKQIKKMDVPVCLIHGCDDNVVPASESRQLHEKFQLLGATSRLILTPLISHGDSRRHL
                     SDLPAAWQLAAGFAFFFKNASTDSRLPDNAR"
     gene            1231..2463
                     /locus_tag="PKK76_13415"
     CDS             1231..2463
                     /locus_tag="PKK76_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69525.1"
                     /translation="MRIDPAFIGFESLEELNRFISDQQLIGAFQFSEPVKDKAGNILI
                     KEKIFVKESAIKRLEAMAGNYDPDFRIAVDPEIIKKLRASLARIIVATLDETESHFIR
                     HLFEKTRSNYNAYIQHAFATDLLVLTFYKQWSDHAEFFRHAAKTGLLALGIIVQKSFG
                     IPRLHRYAFLAGFIAEMPMAASDEWRAQLSDRRRREIAEAASRIAVKLQLPPEVRDAL
                     KNSSLDVEKPANEDVPFDIIDPAHQSGSTEATDDGDDPAPGAPADPASASALTSVVRL
                     ALFIHESSKQIADPAARAEQIVPMVAYNGARGFFHKDLVELIIERFREYEAIARRMMK
                     VAAVEKSCLYPDSAWAYPKPRAAQVLCRHFKTGCPKLESGWDIHVVSPTEAYGWLGTP
                     LAAGNYPKCALERDLPPD"
     gene            complement(2426..3145)
                     /locus_tag="PKK76_13420"
     CDS             complement(2426..3145)
                     /locus_tag="PKK76_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69526.1"
                     /translation="MQTPTYINVYMILASSAVAVAAFGYLRARIESTKERRWWQKSLF
                     VFVALYSIHSVLALSGFYSVFSLPPRFPVAAMATLVIFILFGLSETGRSVLHRIGPDR
                     LALFQVWRFLPEILILLLVRESLLPEAMTAAGRNFDLWIPVTASAMYYLRSRRLLPDA
                     VLIVWNLLGILVLGFTVYTGLRSAPFPFNQPGPIDTSVLAQFPIAFLPLFMVPLAMFS
                     HIAGITLLISNPGADRVPART"
     gene            complement(3235..>4111)
                     /locus_tag="PKK76_13425"
     CDS             complement(3235..>4111)
                     /locus_tag="PKK76_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69527.1"
                     /translation="FHVFRHFAFLAAPTLASLVRILSGRTLDGWMTRILAVFSFLLIV
                     AIEIDFFSERSLLIDHWASHEWGTFPVLAIRARILVGSLFLLCAGSSIYWLVNAKESL
                     VRKPVTFPITLWWLAFVGNFPALLGVGLYPVGMIFETIVSVLIAATLRKNSANHWSQK
                     ASEVMACSSLSLTIGVLLSALMPENHFKAIAVSLAAGIAAFLGTDRLRSKQIEKKIPG
                     LASYQLTRQEERICELVAEGYTRAQIGFFLGIADGTLRNHLVHLYAKTVDREKESGVR
                     DKFQRLTLFLLNLQK"
BASE COUNT         1067 a          978 c         1102 g          964 t
ORIGIN      
        1 gaatgcgaaa aacacaagat gcatgatcca tgtaagcgtt gcgctgaaag ctgtcgcgca
       61 tgcatcgctg ccatgaaagc agcctaggat ttagcgggca ttgtctggca atcggctatc
      121 ggtggacgca ttcttgaaga agaaagcgaa tccggcagct aattgccagg ccgccggaag
      181 atcggaaaga tgacgcctcg agtccccgtg tgaaatgaga ggagtgagaa tcaatcttga
      241 agtcgccccc aggagctgaa atttttcatg caattgccgc gactcactgg caggaactac
      301 attgtcgtcg cagccgtgga tcaggcagac aggtacatcc attttcttga tttgcttcat
      361 ggcgcatatt tgcgctagaa tcgagcgacg tttgccgctc ttctttatga ttcttttcca
      421 gtgacgcatt ctaaagttcg aatcttcccg cagtcgcaat agaaatgttc tttctgtccg
      481 tcgcagagtc ttttcgtaat cagggaaaac agggtctctt gcgtgaaaat agctgtcgtg
      541 aaggatatgt gtgatcgcag ttgcaatacg cggctgtctt ccggcagaca ggtgtagaaa
      601 gtttcttaga atgatcagtc gcccgtattc gtctgcattt tgattgagca tcaaagagtc
      661 cagaagagca tccacatttg cgtaagtgcc aatggtgcat atagcgctga tgtgctcgga
      721 agaagcagtt gcggccgcga tgagcgtcat gcctccagca aaggatggtg cgagcacagc
      781 tacttttccc gatcgcgtca gatcgcgtcg atgcgagatt gctaacgtta ggcgctgtat
      841 aaggctcacg tgagcagaat cgatttgaag attgcagaaa ccgggtaggt gcggtgatac
      901 gacggtgaat ccgattccag cgagggcgct gtttaaatgg aggaatctct catcgcgatt
      961 cccgagaggg gcgaatccgt tgatggtcaa gatgcatcct ttgctcggtc cgatcgggtc
     1021 gtatatatct actccgatgc tttcgtcatt gattgtgata tattctgatg agtaggattt
     1081 cctctgcttc aggctgagtc ggatcagaaa accgaaagct gccaggatgt ttcgcatgat
     1141 tccatcgtag tgcacccatg taacaaacag gagtcggaaa tcgtacaaaa atatggtggc
     1201 taaaaaggcg cgggaccgaa cctttcaccc atgcgcattg atccagcatt cattggattt
     1261 gagagtctgg aagaattgaa tcggttcatt tcggatcagc aattgatcgg tgcatttcag
     1321 ttttctgaac cggtgaagga caaagcaggc aacattctta ttaaggaaaa gatctttgta
     1381 aaggagagtg ctatcaagcg cctggaagcg atggcgggca actatgaccc ggattttcgc
     1441 attgcagtgg accctgaaat cattaagaag ctacgtgcaa gtcttgcccg catcatagta
     1501 gccactctgg atgaaacgga gtctcacttc attcgtcact tgtttgaaaa gaccaggtca
     1561 aactacaacg cgtacatcca gcacgcgttt gcgactgact tgctggtcct tacattctac
     1621 aaacaatgga gtgatcatgc ggagttcttt agacacgccg caaagacggg acttctcgca
     1681 ctcgggatca ttgtgcaaaa gtcgtttgga attccgcgtc ttcatcgtta tgcttttctt
     1741 gccgggttca ttgcggaaat gccaatggcg gcttcagatg agtggagggc gcagctgtca
     1801 gatcgccgtc gccgcgagat tgcggaagct gcgtccagga ttgctgtgaa gttacagcta
     1861 ccacccgagg tcagagacgc tctcaagaat tcatccctgg atgtggaaaa gccagcgaac
     1921 gaagacgtac cattcgatat tattgatcct gcgcatcagt ccggtagcac ggaagcgacg
     1981 gacgatggtg atgatcctgc gccaggtgcg cccgcagatc cagcgtccgc ttctgcactc
     2041 acaagtgtcg ttcgcctcgc tcttttcatt catgaatcga gcaagcaaat tgccgatcca
     2101 gcggcgaggg cggagcaaat tgttcccatg gttgcctaca acggtgcgcg aggatttttc
     2161 cacaaagacc ttgtggagct tatcatcgaa cggttccgcg aatatgaagc cattgcacgt
     2221 cgcatgatga aggtagctgc agtggaaaag agttgtcttt atcctgattc tgcctgggcg
     2281 tatccgaagc cgcgcgctgc gcaagtgctc tgcagacatt tcaaaactgg atgtcccaaa
     2341 ctggaatcgg gatgggacat ccatgtagtg tcacccacag aagcatacgg atggctgggc
     2401 actccgctcg ccgcgggcaa ttatcctaag tgcgcgctgg aacgcgatct gcccccggat
     2461 tagaaatcag aagagtgatt ccggctatgt gcgaaaacat agccagcggc accatgaaga
     2521 gcggaagaaa agcaatcgga aattgagcca gaacggatgt gtcaatcgga cctggctggt
     2581 tgaaaggaaa cggtgcagat ctgaggcctg tatatacagt gaatcccagg acaagaatcc
     2641 ctaacagatt ccagacgatc aggactgcat ctggcaagag tctgcgcgat cttaagtaat
     2701 acatagcaga tgcagtgacg gggatccata gatcgaaatt tcttcccgcc gcagtcatcg
     2761 cttcgggcaa aagtgactct cgcacgagca acaggatgag tatttcaggc agaaagcgcc
     2821 agacctgaaa aagggcgagg cggtctgggc cgattctatg aaggacagac ctgccagtct
     2881 cgctcaggcc aaacagaatg aagattacta gagtggccat ggccgccacg ggaaagcgtg
     2941 gcggcaggct gaacactgaa tagaatccgc ttaacgctag cactgaatga atggagtaga
     3001 gcgcgacaaa gacaaagaga gacttctgcc accatcggcg ttctttggta ctttctattc
     3061 tggcgcgcaa atagccgaat gcggccacgg ccactgcact gcttgcgagg atcatataaa
     3121 cgttgatgta ggttggtgtt tgcatgcatt cattctagga caggagcgtg attttgaaaa
     3181 taggccgcct gtcacgaatg tgaccaggaa ccgtgacatc tgtcacggtt ttcgctattt
     3241 ttgcagattg agaaggaaca gagtcagtct ctggaatttg tcgcgcactc cggattcttt
     3301 ttcgcgatcc acagtctttg catacaaatg gaccaggtga tttctgagag taccgtccgc
     3361 gattccaagg aaaaagccaa tctgagcgcg agtgtaaccc tctgctacaa gctcgcaaat
     3421 tctctcttcc tgtcttgtca attgatagga tgcaagcccg gggatcttct tttctatttg
     3481 tttgcttctc agccggtctg ttcccagaaa cgctgcgatc cccgccgcca gcgacactgc
     3541 aatggctttg aaatgatttt ctggcatgag tgccgaaagg agcaccccaa tggtcagcga
     3601 aagagagctg caggccatga cctcagatgc cttctggctc cagtgattcg ctgagttctt
     3661 tctgagtgtg gctgctatca gcacgcttac gattgtttcg aagatcatgc ccacggggta
     3721 aaggcccact cccaggagtg ccggaaagtt tcccacaaac gcaagccacc acaatgtgat
     3781 tggaaaggtg acaggttttc gcactaaact ttctttagca tttacaagcc agtagatgga
     3841 gctgcccgca catagaagaa aaagtgaacc aacaaggatt cgtgcgcgaa tggcgagtac
     3901 cggaaacgtg ccccattcat gcgaggccca gtggtcgatc agaagagacc gttctgaaaa
     3961 aaaatcaatc tctatcgcga caatcagcaa gaaagaaaaa actgcgagga ttcgcgtcat
     4021 ccatccatcc agagtgcggc cagagagaat gcgaacgagg gatgctagag tgggtgctgc
     4081 cagaaatgcg aagtgccgaa atacgtgaaa c
//
LOCUS       DAORFF010000356         4106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13031_length_4106_cov_3.190323, whole genome shotgun sequence.
ACCESSION   DAORFF010000356 DAORFF010000000
VERSION     DAORFF010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13031_length_4106_cov_3.190323"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1490
                     /locus_tag="PKK76_13430"
     CDS             <1..1490
                     /locus_tag="PKK76_13430"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNL69528.1"
                     /translation="YPDSPRNGSELCAELGEAMLETGADQILILSWNPRGVFADRGAG
                     DLRRLIRSWPDLRPEGEDGYVTVYHAALEGEDKNKLCSILAGLIALLKKYYAAVIIDP
                     GIDHSDSLVREALEQADRVVLVQSCRDPSVLRSKVFAETISSCVKADSDFFSRAILVT
                     HESPGEGALPHIVEGRFERHLRLRGVSERIEETDRTTRSGVRRIARALLGVSRALCLG
                     GGGARAFAHIGVLEVFEENGIEFDAVAGTSMGAVMAGAYALGLSAEEIEYNVRRILPH
                     SSAILDKTIPLVSFFTGRNLNRTILRFFGDLKFEDLSLPFFCNAVDLNSGKEIVFEKG
                     YLAAAIRASVSIPGVFPPFRHGPYALVDGAVLNNLPGDILKSRGYDIILGVNVSPLED
                     HRPVSFGIQTRKGWVRGIRDYFSIPPILRIINRSIAIESRQLLKIHMEQFEYLLHPEV
                     SDFDTFDFERKKEIIDAGRTAARTHIGEIKDTFTRQSLGRREHER"
     gene            complement(1494..2045)
                     /locus_tag="PKK76_13435"
     CDS             complement(1494..2045)
                     /locus_tag="PKK76_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNL69529.1"
                     /translation="MPRVLVPLADGFEEMEGIIIIDVLRRAGIEVVSASLKDGPITAA
                     RGTKHLADTTLDQALSQEFDMLVLPGGGPGAKALEADERVAQLLKEFHEKKKRIGAIC
                     AAPNALRRHNVIQGQDRFTMYPDSVQGGNGGFYTKERVVRNAHVTTSLAAGSAFEFAL
                     DIVEQLLGPEKKAAVAGPMFLPQ"
     gene            complement(2052..3050)
                     /locus_tag="PKK76_13440"
     CDS             complement(2052..3050)
                     /locus_tag="PKK76_13440"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNL69530.1"
                     /translation="MKHIAIVGATGAVGQEMIHVLEKRNFPVSKLTLLASKRSAGKTM
                     TFRGQAVVVQELTEQSFAGVDIALFSAGATMSKQFAPAAVKAGAVVVDNSSAFRMDPG
                     TPLVVPEINPEDIKLHKGVIANPNCCAIILLMAVYPIHKINPIKRIIVSTYQSASGAG
                     AKAMEELKKQAADVLAGRAPVPEVFQFPIAFNVFSHNAGMDLESGYNGEELKVIEECR
                     KILHSDIPISPTCIRVSTMRAHAESVHLELTSSADLDAIRNAISSSPGVRLQDDRKNN
                     HFPMPLEASGQDDVLVGRIRKDIGSTSGKEIDLFCCGDQLLKGAALNAVQIAEKLS"
     gene            3110..3892
                     /locus_tag="PKK76_13445"
     CDS             3110..3892
                     /locus_tag="PKK76_13445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNL69531.1"
                     /translation="MKQVKRERAGLLWRLLPASARRKIAAYLTLSELQDLNESYRQYT
                     KQDPARRRTVQMELSSQIRTRRSIWPAAVSLTLTLVSVFLWIVQGKFGMSESLRLALF
                     GPLVMTAASPLSLYFLSPVRLRMLFRLPQTFESVAVCIVTMVFLIWLLYLIGESGGGV
                     LRVDRLTFIVLILGAMGAPLLEEVLFRELIPSMAPDILAGHALAVPIFAIAHFPDSPS
                     MFALYILAGGFLSMLRIQSGGLLLPLVAHAMANGIVTAARFG"
BASE COUNT          900 a         1029 c         1078 g         1099 t
ORIGIN      
        1 tttatccaga cagtccgcga aacggaagcg agctttgcgc cgagcttgga gaggccatgc
       61 tcgaaacagg agcggaccag attctcattc tatcctggaa tcctcgcgga gtattcgcag
      121 atcgcggtgc cggcgatctt cgccggttga ttcgatcctg gcccgatttg aggccggaag
      181 gcgaggatgg atacgttact gtctatcacg cggcactcga aggcgaagat aaaaacaaat
      241 tgtgcagcat cctggcaggc ttgatcgctt tgctcaagaa atactatgcc gccgtaatca
      301 tagatccagg tatagaccac tctgattcgc tcgtaagaga agcactcgag caggcagatc
      361 gtgttgttct agtccaatcg tgtcgtgacc cctctgttct tcgcagcaag gtatttgctg
      421 aaactatttc gagctgcgta aaagcagact ccgatttctt ctcccgggcc attctggtga
      481 cacatgaatc tccgggggaa ggagcattgc cgcatatcgt ggaaggaaga ttcgagagac
      541 atcttcgcct tcgcggagta agcgaaagaa ttgaggaaac agatagaacc acgcgcagcg
      601 gcgtacgacg aattgctcgc gcactgctcg gagtatcaag agctctctgt ctggggggtg
      661 gaggtgcgcg ggcatttgca catattggcg tccttgaagt attcgaagaa aacggaattg
      721 agtttgatgc tgtggcgggc acttccatgg gcgcagtcat ggccggagcg tatgctcttg
      781 gtttgagtgc ggaagagatt gaatataacg tcaggcgaat attgcctcac tcttctgcga
      841 ttctggacaa aacgattcct ctcgtaagtt tcttcacggg cagaaacttg aaccggacca
      901 tcctcaggtt tttcggagac ttgaaatttg aagatctcag tttgccgttt ttctgcaatg
      961 cggtggatct gaattcaggc aaagagatag tttttgaaaa agggtatctc gcggcggcca
     1021 ttcgcgcaag cgtcagtatc ccgggagtgt ttcctccttt cagacatggg ccctacgctc
     1081 tcgtagatgg tgctgtttta aacaatctgc ctggagacat tctcaaatcc agaggatacg
     1141 atattatcct gggagtgaat gtttcccctc tcgaagacca tcgtcctgtt agttttggaa
     1201 tccagactag aaagggatgg gtgcgtggga tcagagatta cttttctatt ccacccattt
     1261 tgcgcatcat caaccgatcg atcgccattg aaagccgtca gctattgaag attcatatgg
     1321 agcagtttga gtatttgctt catccagagg tttcagactt tgataccttt gattttgaac
     1381 gaaaaaaaga aatcattgat gcgggtcgaa ccgccgcgcg cacacacatc ggagagatca
     1441 aagatacatt tacccggcag agcctggggc ggcgcgaaca cgaacgttag gcgctattgc
     1501 ggtagaaaca ttggcccggc cacggcagcc ttcttctctg gaccaagcaa ttgttccaca
     1561 atgtccaatg caaattcgaa tgctgaaccg gccgcgaggc tggttgtaac atgcgcgttt
     1621 cgcacgactc tttcctttgt gtaaaatccg ccatttccgc cctgaactga atctggatac
     1681 atggtaaagc gatcctggcc ctgaatcacg ttgtgtctgc gaagtgcatt gggcgcggca
     1741 cagatggcgc cgattctctt ctttttttcg tggaattctt tgagaagttg tgccactctt
     1801 tcgtccgctt ccagggcttt ggcgcctggt ccgcctccgg gtagaaccag catgtcgaat
     1861 tcctggctca aagcctggtc gagcgtagtg tctgccagat gctttgttcc gcgcgctgcc
     1921 gtgattggtc cgtctttaag agaagcggaa actacctcaa tgcctgcacg tctgagcaca
     1981 tcaatgatga tgatgccctc catttcttcg aagccatctg caagtggaac gagaactctt
     2041 ggcatagaac ctcagctgag cttttctgcg atttgcaccg cattgagagc ggcgcctttc
     2101 agcagttgat ctccgcagca aaaaagatca atctctttgc cacttgtgga tcctatgtct
     2161 ttacgaattc tgcccaccag gacatcgtcc tggccggatg cttcaagagg catggggaag
     2221 tgattgttct ttctgtcgtc ctggagtctc actccagggg aagaagagat ggcattgcga
     2281 atcgcatcca ggtccgcgct acttgtcagc tccaggtgaa cagattccgc atgagcgcgc
     2341 atggtggaca cgcgaatgca agtgggagaa atgggaatgt ctgaatgcag aatttttctg
     2401 cactcttcga ttaccttgag ttcttctccg ttgtacccgc tttccaaatc catgcctgcg
     2461 ttgtgactga agacgttaaa cgctattgga aattgaaaaa cctctggcac tggcgcgcgg
     2521 cctgcgagca cgtccgctgc ctgcttcttc agttcttcca ttgcttttgc gcccgccccg
     2581 gatgcggact ggtatgtgga tacaatgatc ctcttgatag gattgatctt gtggattggg
     2641 tacactgcca tgagcaagat aatcgcacag cagtttgggt ttgcgatgac acctttgtgc
     2701 agcttgatgt cttccggatt gatttcaggt acgacgagtg gagttcccgg atccatcctg
     2761 aatgcggaag agttgtctac gaccacggcc cctgctttga ccgcggcagg cgcgaactgc
     2821 ttgctcatgg tggcccccgc gctgaacaaa gcaatgtcta cgccggcgaa agattgttct
     2881 gtgagttcct ggacgaccac ggcctggcca cggaatgtca ttgtctttcc agcggaacgt
     2941 ttgctggcca gaagagtgag ctttgaaacc ggaaaatttc tcttttcgag aacgtggatc
     3001 atttcctgac ccactgcacc tgttgcaccg acaatagcta tatgcttcat tctttctctc
     3061 ctggcacctg ctcgcgcaaa acgggcatcc aatcggattt tttgttccta tgaagcaggt
     3121 caagcgagaa cgagcgggct tgctctggcg cctgttgcca gcttctgcca ggcgaaagat
     3181 cgcggcctat ctgacacttt ccgagcttca ggatctaaat gaatcctatc gtcagtatac
     3241 caaacaggac cctgcccgtc gacgaactgt gcaaatggaa ttgagcagtc agattcgcac
     3301 ccgaagatcc atttggccgg cggctgtcag tctcacactc accttagtct cagtttttct
     3361 ctggattgtt caggggaaat ttggaatgag cgaatccctt cgtctggctt tgtttggacc
     3421 gcttgtgatg acagcggctt ctcctttgag cctgtacttt ctatccccgg ttcggcttcg
     3481 catgctgttt cgacttccgc aaacctttga aagtgtggct gtgtgcatag taacgatggt
     3541 atttttgatc tggcttttgt atttgatcgg agaaagcggc ggtggagtac tgcgtgtaga
     3601 tcgcctaacg ttcattgttt tgattctggg tgccatgggt gcgccgcttc tggaagaagt
     3661 tctattcagg gaactcattc cttctatggc gcctgacatt ctggccggcc atgctctggc
     3721 cgtgccgata ttcgccattg cacattttcc agattctcct tccatgttcg ctctctatat
     3781 tcttgcaggc ggttttctat cgatgcttcg aattcaaagc ggtggattgc ttttgcctct
     3841 ggtggcccat gccatggcga acggcattgt aacggccgcg agatttggtt gatgttcctg
     3901 atgccacagt tcaatctgca aaagaacaat ggcagagaat cttgacatcg tagtgttcgg
     3961 aggatctttt gatccaccgc atgcaggtca tgagcaaatg gcgcggctgg cccttacgcg
     4021 cgcgagtcgt ttgatcttca tgcctgcgca tcgatccccg ctcaaacatg cgcaacccgc
     4081 gcgttttgaa caacgtgtaa ggatgc
//
LOCUS       DAORFF010000357         4100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13062_length_4100_cov_4.123362, whole genome shotgun sequence.
ACCESSION   DAORFF010000357 DAORFF010000000
VERSION     DAORFF010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13062_length_4100_cov_4.123362"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1324)
                     /gene="trpB"
                     /locus_tag="PKK76_13450"
     CDS             complement(149..1324)
                     /gene="trpB"
                     /locus_tag="PKK76_13450"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNL69532.1"
                     /translation="MGSEKSGFFGEFGGRYSPEVLMPAILELEEVYFKVRSKKRFQKK
                     WEQLQRDYIGRPSRLTYAARLSDEWGAQIYLKREDLNHTGAHKINNAIGQALLALEMG
                     KERIIAETGAGQHGVATATAAAHFGLKCTVYMGSEDVRRQKLNCYRMKLLGAEVIPVT
                     SGTSTLKDATNEAMRDWARNVRNTHYIIGSAIGPHPFPIIVADFQSVIGKESRKQMKK
                     GPGLPHAVIACVGGGSNAIGIFHGFLKDDVDLYGVEAGGRSDDPGENSASITFGEVGY
                     FHGTKSLFIQKEGGQIQGVHSVSAGLDYPGVGPEHAYLAKTGRVKYVRVRDDRALECF
                     QEVSRLEGIIPALESAHALAFARELARDAGKKARILVNLSGRGDKDVAEAASILGME"
     gene            complement(1354..3078)
                     /locus_tag="PKK76_13455"
     CDS             complement(1354..3078)
                     /locus_tag="PKK76_13455"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNL69533.1"
                     /translation="MRASSLLIHTLREPPADAVLASHKMMLRAGLVSKLGSGLYNMLP
                     MGLRVFRKIENIIREEMNAAGGQEFVMPILIPAELWETSGRWNAMGKEMFRIKDRHET
                     WNVLGPTHEESFTDAMKGILKSYRDLPVNVYQIHTKFRDEIRPRFGVMRSREFVMKDA
                     YSFHKDEECLGRTYQVMRKAYRNIFGRLGLETIPVEADTGTMGGSRSEEFMVPSEVGE
                     ETLLISAGKKYFSNQEKTPFVAGKVEKPPALVKSAKKHHTPNAKSIEDVARFLGVSSV
                     HLAKALIYRVGESYVLVFIRGDRAVNDVKLKNALGGEEFRAATEDEVRSLGFVPGFIG
                     PGHVPEGMRIIQDSSIESGRPYVVGANEVDHHRSGHVFEANPDARDVALAVEGDRSPM
                     GDGVLHAIKGIEVGHIFQLGTKYTKAFGVSVLDESGRPLTPIMGCYGIGVQRTMAAII
                     EQNHDEKGIAWPVSACPFEVVLVSITKGEEEEQKVAALYQKLQKAGLDVLWDDRDLRP
                     GVKFADAELIGYPVRVTVGKTFFESGKLEIQLRKTGEKVETTPAEMVKTIRNLTRSLK
                     RKHPPKKR"
     gene            complement(3080..>4100)
                     /locus_tag="PKK76_13460"
     CDS             complement(3080..>4100)
                     /locus_tag="PKK76_13460"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNL69534.1"
                     /translation="GRALIGLRAPGERNLKVNYPSRLVWAYKLQSFLGHTELPAGLRA
                     YPYLNNGDVILSVQGRNVKSATELQDLLGEFHGQPVQIRVKRQTLSWLAPWFTEETTV
                     TVPSQAEYRIELHNLVDLKYGTPVADQVLLSNVEAHQRALSILSIDGKPPGSYEKMYE
                     ALATERAITLTLKDRNYSAKAHAAKIGLLGFRPDSRIETTYLPGHGSVSEVISHAFGD
                     TAKNVMVYPAFFGSLISGRLSFIDNAMGPVGMFAAAGVVLESGIQDYLGMFAAISIAL
                     FIMNLLPFPVVDGGHVVFFLYEAVRGKPVTPGIMEGIYRGGLAVLVFMGLWIMYRDIL
                     FISGL"
BASE COUNT          960 a         1126 c          956 g         1058 t
ORIGIN      
        1 ccaagctcga tgagatctgc acctgcatca atcatcccgc aggcgaagcg aaccgtggag
       61 gcaaaatcag gatcccctgc agtcagatac ggcatgtaga gaggcccctg ggctctcttc
      121 ttcaatatgt actctttgag tttatccatc attccatacc cagaatagaa gccgcttctg
      181 ctacgtcctt gtcaccacgg ccggaaaggt tcacgaggat gcgcgctttc tttcccgcgt
      241 cgcgcgccag ttcccgcgcg aaagcgagtg catgggcgct ctcgagtgcc ggtatgattc
      301 cttcgagcct ggatacttcc tgaaagcatt ccagagcgcg atcgtctcgg acgcgcacgt
      361 acttcactct acccgtcttt gccagatacg catgttctgg tccgactcct ggataatcca
      421 gccccgcaga tacgctgtgc acaccctgga tttgtccgcc ctctttctgt atgaaaagag
      481 actttgtgcc gtggaaatag cccacttcgc caaaggtaat gctcgccgag ttttcaccag
      541 gatcatcgct tctgccgcct gcttccactc cgtagagatc tacatcgtct ttcaaaaatc
      601 cgtgaaagat tccaattgca ttggatccgc cacccacgca tgcgatgact gcatgaggaa
      661 gaccagggcc tttcttcatt tgcttgcgag actcttttcc aatgactgat tggaagtctg
      721 ctacaatgat tggaaacgga tgagggccaa tggcagagcc aatgatgtaa tgagtgttgc
      781 gaacgtttct cgcccagtct ctcattgctt cattggttgc atctttgaga gtagacgttc
      841 cgcttgttac aggaatcact tccgcaccca gaagtttcat gcgataacaa ttcaatttct
      901 gacggcgcac atcttcgctg cccatgtaaa cggtgcattt caatccgaaa tgagcggctg
      961 ctgtagccgt ggccacccca tgctgtccgg ctccggtttc agcaatgatc cgttcctttc
     1021 ccatttcgag agcgagcaat gcctggccga tggcgttgtt gatcttatgc gctccggtgt
     1081 gattgaggtc ttcgcgcttg agatagattt gtgcgcccca ttcgtcgctg agcctggctg
     1141 catacgttag acgagatggt ctgccaatgt agtctctttg cagctgttcc cactttttct
     1201 gaaaacgctt cttactgcga accttgaagt agacttcttc cagttccagg atcgcaggca
     1261 tgagaacctc ggggctgtac cttccaccga attctccaaa aaaaccgctt ttttcagaac
     1321 ccatgagcca gcgttggcgt catgccctgc acttcatctc ttttttggcg ggtgctttcg
     1381 cttgagcgat ctggtaagat tgcgaatggt tttgaccatt tcagcaggcg tggtttctac
     1441 tttttcgccc gtcttacgca attgaatctc tagttttcct gactcaaaaa aagttttacc
     1501 gactgtaacg cgaacaggat atccaatgag ttctgcatct gcgaatttca cgcctggtct
     1561 gaggtccctg tcgtcccaca aaacatcgag gcccgctttc tgtaacttct ggtagagcgc
     1621 cgctaccttt tgctcttctt cttctccctt tgtgatgctt acaagcacca cttcaaaagg
     1681 acaggcactc accggccagg caattccctt ctcgtcatgg ttctgctcaa tgatggcagc
     1741 catggttctt tgaacgccaa ttccgtaaca acccatgata ggagtgagag gcctgcctga
     1801 ttcgtctaga acggacacac caaatgcttt tgtgtacttc gttccaagct ggaagatatg
     1861 acctacctca atgcctttga tcgcatgcag tactccatcg cccataggcg aacgatcccc
     1921 ttctactgcg agtgccacat cgcgcgcgtc tggatttgct tcgaaaacgt gaccgcttct
     1981 atgatgatct acttcatttg ctcccactac ataaggacga cccgactcaa tggacgaatc
     2041 ctgaatgatg cgcattcctt ctggcacatg acccgggcca atgaaacctg gaacaaaacc
     2101 aagggaccgc acttcgtctt ctgtggcagc gcgaaactcc tctccaccca gggcattctt
     2161 caacttcacg tcattgacag cgcgatctcc gcgaatgaag acaagaacat aggattcgcc
     2221 cacgcgatag atcagagctt ttgccagatg aacggacgac acaccgagaa accgggcgac
     2281 atcttctatg gacttagcat tcggagtatg atgcttcttc gctgatttta caagtgccgg
     2341 tggcttctcc acttttcccg caacaaacgg agtcttctcc tgattgctga agtatttctt
     2401 cccggccgaa atcaaaagcg tctcttcccc tacttcagaa ggaaccatga actcttcgga
     2461 tctcgatccg cccatggttc cagtatctgc ttctacggga atggtctcaa ggccaagcct
     2521 gccgaatata ttgcgatatg ctttgcgcat cacctgatac gttcgaccca gacactcttc
     2581 gtccttgtgg aacgaatacg catccttcat tacaaattcg cgcgatcgca taacaccaaa
     2641 ccgcggacga atttcatcgc gaaacttggt gtgaatctgg tacacattta cgggaagatc
     2701 tcgataggat ttcaaaatcc ctttcatcgc atccgtgaag gactcttcgt gtgtgggtcc
     2761 aagaacgttc catgtttcgt gtctgtcttt gattcgaaac atttccttgc ccatggcatt
     2821 ccatctgccg gatgtttccc acagttcggc cggaatcagg atgggcatga caaactcttg
     2881 accgcccgcg gcattcatct cttcgcgaat gatgttttca atcttgcgaa agactcgcag
     2941 gcccatggga agcatgttgt aaagaccact accgagcttg ctgaccaggc cggcgcgtag
     3001 catcatcttg tgtgacgcga gtaccgcatc cgccgggggc tcccggagag tgtgaatcaa
     3061 aagagaactt gcgcgcattc taaagtccag atatgaagag tatgtcgcgg tacatgatcc
     3121 acagtcccat gaagacaaga actgcaagac caccgcggta gattccttcc ataattccag
     3181 gcgttacggg tttaccgcgg acagcttcgt acagaaagaa tactacgtgg ccgccgtcaa
     3241 caacagggaa cggcaaaagg ttcatgatga aaagcgcaat agaaatcgca gcaaacatac
     3301 ccaggtagtc ctgaattcct gattcaagca ctactccggc cgcagcaaac atcccgacag
     3361 gccccattgc gttatcgatg aaagaaagtc tgcccgaaat caggcttcca aaaaatgcag
     3421 gatacaccat gacgttcttc gcagtatcac caaaggcgtg cgaaatcact tcgctaacgg
     3481 aaccatgccc cggaagatac gtggtttcaa tgcgcgaatc cggtctaaat ccaagaaggc
     3541 caatttttgc ggcgtgcgcc ttcgcagaat agttgcgatc ctttagtgtc agagtgatgg
     3601 cacgttctgt tgccagagct tcgtacattt tttcgtaaga accaggaggt tttccgtcta
     3661 tagaaagaat gctgagtgca cgctggtgtg cttccacatt gctgagcaat acctgatctg
     3721 ccacgggagt tccgtacttc aagtcgacaa gattgtgcag ttcaattctg tattctgcct
     3781 gcgatgggac tgttacggtg gtttcttctg taaaccacgg agctagccac gacaacgttt
     3841 gtctcttcac acgaatctgc acaggctgtc cgtgaaattc tccgagcaaa tcctggagtt
     3901 ctgttgcgga ttttacattt cttccctgca cggaaagaat gacatctccg ttgttcaaat
     3961 aagggtacgc cctgagtcca gccgggagtt cagtatggcc caggaaggac tggagcttgt
     4021 acgcccacac caggcgagac ggataattta ctttaagatt tctttctccg ggtgcgcgaa
     4081 gaccaatgag agcgcggcct
//
LOCUS       DAORFF010000358         4081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13153_length_4081_cov_3.571287, whole genome shotgun sequence.
ACCESSION   DAORFF010000358 DAORFF010000000
VERSION     DAORFF010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13153_length_4081_cov_3.571287"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PKK76_13465"
     CDS             complement(<1..231)
                     /locus_tag="PKK76_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNL69535.1"
                     /translation="MSDHVPQHQKSRAFMPVEKKNGLPNSLRFPGSMFDLASYLRTGP
                     SIALVGATNDTTKYGNIILRDLVSKGYTVIPVN"
     gene            213..905
                     /locus_tag="PKK76_13470"
     CDS             213..905
                     /locus_tag="PKK76_13470"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HNL69536.1"
                     /translation="MERDLTFGSLKLKHPEVIAVVERILPSDRFDELAEQDLRILELR
                     FDSFSEPWSDVVAFAQSMRSRFAILGTCRENEENRNLLKQRYADFVPVVDCVDIEIGT
                     ERALRSELIRITKDSSRQLMLSHHDFEKTPPDAELDRTIDDALLLRADFVKLALAAPS
                     RQDAARLMFLLERRRDAHLSAFSMGSAGAVTRIAGGLFGSIFTYGYMDHPNAPGQLSA
                     RELLALRKKFYS"
     gene            996..1583
                     /locus_tag="PKK76_13475"
     CDS             996..1583
                     /locus_tag="PKK76_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNL69537.1"
                     /translation="MTKLKTAVVLTTALSAAALFAGDVQLDKSHTKVEFNVTHLTISD
                     VTGRFDEFEGKGSFDEKTGVLSALDVKIKVKSINTNDEKRDDHLRSADFFDASKYSEI
                     TFVLTKPVTLVKGGTANLQGNLTMRGVTKPITLQMNYKGTVKDPWGNVKAGFILTGKV
                     NRKDFGVSWSKNMDNGGAVVGDIVTINIAGEAAVK"
     gene            1670..2680
                     /locus_tag="PKK76_13480"
     CDS             1670..2680
                     /locus_tag="PKK76_13480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HNL69538.1"
                     /translation="MTTMKAAAMQSFGGPEKINISDLPVPAIGPGEMLVRMKSASINP
                     IDWKTMLGKVKILIQYPFPLVPGSDGAGVVETIGPGVSRFKVGDRVYFRPGKDRIGTF
                     SQYFVVRETEAAVFPSKLSFDEAASLPLAGLTSWQALDLANVKAGDQVLIHAGAGGVG
                     ALAVQLARARGAIVTATCSAKNADFVRSLGATNIIDYRTEDYTSGGKRFDFVFDTQGG
                     KETNRCMDVLKPGGMVVSIANPPDPIEAVKFGRPWYVKLLLQLANIAPNRAARKRGVQ
                     YRALLMDASSSQLEEMAKLIEKGSLKPVIDKIFPFAETRDAIAYQMLGHSRGKVIIHI
                     ED"
     gene            complement(2692..>4081)
                     /locus_tag="PKK76_13485"
     CDS             complement(2692..>4081)
                     /locus_tag="PKK76_13485"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL69539.1"
                     /translation="IFAGASASLIFSALTHYLIHYGRLTHKVKIFASVFDVAIVSIAL
                     HQFGEYRTFKSEAYLLYFVWIALVNLRFTPVLTILASTSAIFFYALLALIAVQTGTIQ
                     TGSLYDSYTSGSVSLTSVALKLLFLSIFAVAAVQAARGNRRLINKSVETELEAARQHE
                     DRMKAQFASLAQTKQMLDSFSRFVPARFLEFLHVKDMVDIELGACVEKEITLLFVDIR
                     NFTGMSEKMSVFDNFRFLNAYLKRMGPIIEQQGGIIDKFVGDEIMCLFPDSPQKALVS
                     SIEMRRELAIYNTDRKKMNYDALDIGAGLHTGNVMLGTVGSENRISTTVIGDNVNLAS
                     RLQTLTKIFGVPIILSDAVYRRLDDPSAFCLREIDSVLVRGKEKPIVLYECFDMDQDE
                     LRDQKKELHGDLQLALFHYKAGNFAESKQLFVDYEKKCVRDSIPRIYLKRLEKLMQRP
                     TSAKWSGVSRMK"
BASE COUNT         1033 a          957 c         1063 g         1028 t
ORIGIN      
        1 gtttaccggg atgactgtgt agcctttaga aaccaggtcg cgcagaatga tatttccgta
       61 tttcgttgta tcattggtgg caccgacgag agcgatcgac ggaccggtgc gaaggtaaga
      121 ggcaaggtcg aacatagatc caggaaagcg caaactattt ggcaggccat tctttttctc
      181 gacaggcatg aatgcgcggc tcttctgatg ctgtggaacg tgatctgaca ttcggaagtc
      241 tcaaactcaa acatcctgaa gtgattgcag tggtggagcg catcctccca tcggacaggt
      301 ttgatgaact ggcagagcag gatctccgga ttctggagtt gagattcgat tctttttctg
      361 agccctggag tgatgtggtt gcgtttgcac agtctatgcg ttctcgtttt gcgattctgg
      421 gtacatgccg ggagaacgag gagaatcgca atctcctcaa gcaacgttat gcggactttg
      481 ttcctgttgt agattgcgtg gatatagaga ttggaacgga aagggcgctg cgatccgagc
      541 tcatacgaat cacaaaagat tcatccaggc aactcatgct gtcccatcat gattttgaga
      601 agactccgcc ggatgcagag ctcgatcgaa ccatagatga cgctctcctt cttcgcgcag
      661 attttgtgaa actcgccctg gccgcgccat cgagacagga cgcagcaaga ctcatgtttt
      721 tactggagcg ccgccgggac gcgcatctca gcgcattttc catgggcagc gcgggtgcgg
      781 tcacgcgtat cgccggtggt ctttttggat ctatttttac ctacgggtac atggatcatc
      841 caaatgcgcc ggggcagctc tctgcccggg agctactggc cctcagaaaa aaattctatt
      901 cctgacctgg aaatcggtgg tcgcctggcg ccaaatgctt taatatgaaa ctattgaagt
      961 ccgtatggat tcacaagcat tcaatggagg gtcccatgac caaacttaag acagcagtcg
     1021 ttcttaccac agcactttcc gcagccgcac ttttcgcggg agatgtgcaa ctggataaat
     1081 ctcacacaaa ggttgagttc aatgtaacgc atctgacgat ttcagatgtg accgggcgct
     1141 ttgacgaatt cgaaggcaag ggaagtttcg acgagaagac gggagttctt tccgcactcg
     1201 acgtgaaaat caaagtgaaa tccatcaaca caaacgatga aaagagagac gatcacctga
     1261 gaagcgctga cttctttgac gcgtccaaat actcagagat cacatttgtt cttacaaagc
     1321 ctgtgactct ggtcaaaggc ggaactgcga atcttcaagg caatcttacc atgcgcggcg
     1381 taaccaaacc gatcactttg cagatgaact acaaaggaac cgtcaaagat ccctggggca
     1441 atgtgaaagc tggtttcatc ctcactggaa aagtgaaccg caaagacttc ggcgttagct
     1501 ggagcaagaa catggacaat ggcggcgccg ttgtcggcga catcgtaacg atcaacatcg
     1561 ccggagaagc tgcggttaaa taatcgcaga cggccgtgat cgtaagggcg gctcgtgccg
     1621 cccttttttt tgtctcgcag ggttcgaagt aactcgaatc ctgtggccta tgacaactat
     1681 gaaagcagcc gccatgcagt cgtttggcgg acctgaaaaa ataaacatat ctgatttacc
     1741 tgttccggcc atcggtcccg gtgaaatgct cgtgcgcatg aagtcggcca gcatcaaccc
     1801 gatcgactgg aaaacaatgc tcgggaaagt aaagattctt atacaatatc catttcctct
     1861 ggttccagga agtgacggcg ccggagtcgt tgaaacgatt ggacccgggg tttccagatt
     1921 caaggtggga gacagagttt attttagacc tggcaaagat agaatcggaa cattctctca
     1981 atacttcgtg gtgcgcgaaa cggaagctgc cgtttttcct tcgaagctga gctttgatga
     2041 agcagcttct cttccactcg caggcctgac gtcctggcag gcgctcgatc ttgcgaatgt
     2101 gaaagctgga gatcaagttt tgatccacgc aggtgcaggt ggggttggtg cactggcagt
     2161 tcagcttgcg cgggcccgtg gagccattgt gactgctaca tgtagtgcga agaacgcgga
     2221 ctttgttcga tctcttggtg cgaccaacat cattgactat cgcacggaag actacacgtc
     2281 aggcggcaaa cgattcgatt ttgttttcga tacgcagggt ggcaaagaaa cgaatcgttg
     2341 catggatgtt ttgaaacctg gcggcatggt ggtgagcatt gcaaatccac ctgatcccat
     2401 tgaagctgtg aagtttggca gaccctggta tgtgaagttg cttcttcaac tggcaaacat
     2461 cgcgcccaat cgcgctgcgc ggaaacgcgg cgttcagtat cgcgctttac tcatggacgc
     2521 gagcagttca cagctcgaag aaatggcaaa gttaatcgag aaaggatccc tgaagccagt
     2581 gatagacaaa atatttcctt ttgccgagac cagagacgcc atcgcatacc agatgctggg
     2641 gcactcgcgg ggaaaagtga tcattcatat agaagattga ttcttccagg attatttcat
     2701 tctactgacg cccgaccatt ttgctgaagt cgggcgttgc atgagctttt caagtcgctt
     2761 caagtagatg cgtggaatgg agtctctcac gcattttttt tcgtaatcga cgaatagctg
     2821 ttttgactct gcgaaattgc ctgctttgta atgaaaaagt gcgagctgca ggtccccgtg
     2881 aagttctttc ttctggtccc tgagttcatc ctggtccata tcaaaacact cgtataagac
     2941 gattggtttt tcttttccgc gcaccagtac tgaatcgatc tcgcgcaagc agaaagcgga
     3001 cggatcgtct aaccgcctgt agacggcatc cgacagaatg atgggcactc caaatatttt
     3061 agtcagggtt tgtagccggc ttgcgaggtt gacgttgtct ccaattacgg tcgtgctgat
     3121 tctgttttca gatccaactg tgcccagcat cacattccct gtgtgaagtc ctgcgccaat
     3181 gtccagtgcg tcgtagttca ttttctttcg atctgtattg tagatggcca attcgcgtct
     3241 catttcaatg gaggagacca aagccttttg cggcgaatcg ggaaacagac acatgatttc
     3301 gtcgccaaca aacttgtcta tgatgccgcc ctgttgttca atgatcgggc ccattcgttt
     3361 caggtaagcg ttcagaaatc ggaaattgtc aaatacactc atcttctctg acatgcccgt
     3421 gaagttgcga atatctacga acagcagagt gatttccttt tccacgcatg ctccaagctc
     3481 aatgtccacc atgtctttga cgtgaagaaa ttcgaggaaa cgcgcaggaa caaaacgaga
     3541 gaaagagtcc agcatctgtt tggtctgagc gagagatgca aactgcgctt tcattctatc
     3601 ctcatgctgt cttgcagctt cgagttctgt ttctacgctt ttgttgatga ggcgcctgtt
     3661 cccgcgcgct gcctgtactg cggccaccgc gaaaatggag agaaatagaa gcttgagggc
     3721 aacgcttgtc aggcttacgg atccacttgt gtaagaatcg tagagactgc ctgtttgaat
     3781 ggttcctgtc tgtaccgcaa tgagagcgag taaggcgtag aagaaaatcg cagaagtgct
     3841 cgcaaggatt gtgagcacag gagtaaaacg caaatttacg agagcgatcc agacaaagta
     3901 taaaagatag gcctctgatt tgaaagtgcg atattcgccg aactggtgca atgcgatgct
     3961 tacgatcgca acatcaaaga cacttgcgaa gatttttact ttatgagtga gtcgcccgta
     4021 gtgaatcaga taatgagtga gtgcggagaa gattagagac gcacttgctc ccgcaaatat
     4081 g
//
LOCUS       DAORFF010000359         4063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13258_length_4063_cov_4.498752, whole genome shotgun sequence.
ACCESSION   DAORFF010000359 DAORFF010000000
VERSION     DAORFF010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13258_length_4063_cov_4.498752"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..820
                     /locus_tag="PKK76_13490"
     CDS             83..820
                     /locus_tag="PKK76_13490"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNL69540.1"
                     /translation="MVPSRQFPEAMAAGDVKRDNYLAAVEKDLRASLGEVSIILRKTI
                     RLDFRMKALQAGCRSILFSPEIISPDERFVPVKDYQQIITYDKLPKGVVSEISVGIYY
                     RKNYMYGFIRAFSPNSFSLAEFDHVSSVARKIEKFFEESNLLPSNPDKCPILDLSMSG
                     AGLLHPQSPIVMQKFMPGESVLLDISFPEGSLNFSASIRNIRSLENAHRIGIEFEGLD
                     SIRTAALEKALGPAVAASQAPGPSPGE"
     gene            complement(821..1126)
                     /locus_tag="PKK76_13495"
     CDS             complement(821..1126)
                     /locus_tag="PKK76_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69541.1"
                     /translation="MDQQVFYSLRDIPEGLDVIDELDLELKWETAIDPERSGAQSFTG
                     IEELNELLKERIRTLLIDKGGECVRVKYEKISHSKNYAHMSLIVVGEYHFEVLGKKK"
     gene            complement(1162..1806)
                     /locus_tag="PKK76_13500"
     CDS             complement(1162..1806)
                     /locus_tag="PKK76_13500"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNL69542.1"
                     /translation="MVELYLQQFARQEADLMSPELAVFNTSRLQALWEAIIAVKGDKA
                     PVPFWCVVWPGARALARYILDHKDQFRGRRVLDAACGSGLAGIAAAKCGARVRGIDIS
                     PEAIELAKHMSHLNGTDCEWVVGDAFAESSELFDIVLVGDVFYDQALARKATKFMRSV
                     TDKGSVVLASDPGRNYCPREEYRAFGNLKVPVDPDLEGADFKITALLTMNRQAN"
     gene            1866..2486
                     /gene="rpsD"
                     /locus_tag="PKK76_13505"
     CDS             1866..2486
                     /gene="rpsD"
                     /locus_tag="PKK76_13505"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNL69543.1"
                     /translation="MATIRKPRHKICRTVGYCLYGSPKCPSVKRPTPPGHHADTRKKK
                     KKSPYGEQLLEKQKLRLTYGLMEKQFYHTFERASRMKGNKSDNFMMLLEGRLMTLVYR
                     LGLARTIFDARQLIRHGHVSVDGQRVDVPSFSVSPGQIIGLTPGSTELDRVKSALETR
                     ASVSSPTPYLEVQSDGVSGKFLGIQSVTDIPVTRINVQKIVEFYSK"
     gene            complement(2539..2889)
                     /locus_tag="PKK76_13510"
     CDS             complement(2539..2889)
                     /locus_tag="PKK76_13510"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNL69544.1"
                     /translation="MDAISIVGHSPTPTRPHGDQTAVRQNEAAAVAAQQTEATEPNVH
                     KIASAVQALNLFHEDKEHTRFQYRVNPEYGTVQVTLFNYMTGEIISEIPSRKVLDFAQ
                     RMREISGMLLEKQA"
     gene            complement(3006..3803)
                     /gene="whiG"
                     /locus_tag="PKK76_13515"
     CDS             complement(3006..3803)
                     /gene="whiG"
                     /locus_tag="PKK76_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HNL69545.1"
                     /translation="MSRLWDKYKDKDETELWRRYRKEANREIRDFLAEKYAPLVKYVA
                     GKVAIGMPQNVEFDDLVSYGSFGLLDAIEKFDPDRDIKFKTYAMTRIRGAIFDELRSI
                     DWIPRSIRQKARQVEETIAMLENKLGHTVEDEDIAKELGISVDELQSLLTKISGTSIV
                     SLNDIWYLGDDNDEVSFMETLESPANLNPDSLIEREEIKSVIVEAIRHLPDKEKKVIV
                     LYYYEDLTLKEIGEVLEVTESRISQLHTKAIMRLRGKLMRMKNALKK"
     gene            complement(3855..>4063)
                     /locus_tag="PKK76_13520"
     CDS             complement(3855..>4063)
                     /locus_tag="PKK76_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004490939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69546.1"
                     /translation="PLSAGDSGGSVDESDSPPQVSTSTGSMPTPSAAPAAYGDHILVN
                     KVKIKNEPKLMAAAIRTLLAKDEK"
BASE COUNT          951 a         1016 c          986 g         1110 t
ORIGIN      
        1 aagtattgaa gcaggagctg caaaaagctg agcaaaagag cctcgcactg aaatagggcc
       61 tgcgaaaata tttgcatcca gcgtggtccc ttctcgccag tttccggaag caatggctgc
      121 cggagacgta aagagagaca actatctggc ggccgtggaa aaggatctgc gagcttctct
      181 aggtgaagtc tccatcattc ttcgtaagac cattcgcctt gattttcgca tgaaagctct
      241 tcaggccgga tgtcgatcta ttttgttttc tcctgagatt atttcccctg atgaacgttt
      301 tgtcccggtc aaggattatc agcaaattat aacttatgat aaattgccca agggagttgt
      361 atctgaaata tcagttggga tttattatag aaaaaattac atgtacggtt tcattcgagc
      421 attttctcct aactcttttt ctctggcaga gtttgatcat gtaagcagcg tagcgcgaaa
      481 gatcgagaaa ttcttcgagg aatcgaattt gcttccatcc aatccagata aatgtcctat
      541 tctggatttg agcatgtctg gcgccggtct tcttcatccg cagtctccga ttgtgatgca
      601 gaaattcatg ccgggcgaaa gcgttctgct cgatatcagc tttccagaag ggagtctcaa
      661 tttttcagca tcgattcgaa acattcgatc tctcgaaaac gcgcacagaa ttgggattga
      721 atttgaggga ttggattcca ttcgcacggc ggcgctggaa aaggctcttg gccctgcggt
      781 tgctgcatct caagccccgg gcccgtctcc gggagaatag ttacttcttt tttcccaata
      841 cttcgaaatg gtattcacca actacgatca aagacatgtg cgcatagttt ttcgagtgcg
      901 agattttctc gtacttcaca cgaacgcatt ctccaccttt gtctatcagt agagtgcgaa
      961 ttcgctcttt caacaactcg ttgagctctt caatgcctgt gaagctctgt gctccggatc
     1021 gctccgggtc gatcgccgtt tcccatttca gttcgaggtc cagctcatcg attacatcaa
     1081 ggccttcggg gatgtctcgc agtgagtaga atacttgctg gtccatgaat cagaatctgg
     1141 cgcggcatgc gcagaatcaa ttcaattcgc ctggcggttc atggtcaaga gtgctgtgat
     1201 tttgaaatcg gctccttcca gatccggatc gacgggcact tttagatttc caaatgcgcg
     1261 atactcttcg cgcgggcaat agtttctgcc gggatctgat gccagtacca ccgatccttt
     1321 atccgtgacg cttcgcatga acttcgtagc tttgcgcgca agggcctgat cgtaaaagac
     1381 atcgcccacc aggacaatat caaatagctc ggacgattcg gcgaacgcgt cccccaccac
     1441 ccattcgcaa tcggttccat tcagatggct catgtgcttt gccagttcga ttgcttctgg
     1501 actgatgtcg attccgcgga ccctggctcc gcattttgct gccgcgattc ccgccagacc
     1561 gcttccgcac gcagcgtcca gaacccgtcg ccctcgaaac tggtccttgt ggtccagaat
     1621 gtaacgggcc agggcgcgtg ctcccggcca gactacgcac cagaacggaa cgggagcctt
     1681 gtctcccttt acagcgatga ttgcttccca gagcgcttgc agcctggagg tattgaaaac
     1741 agccagttca ggggacataa gatctgcttc ctggcgtgca aattgctgta agtagagttc
     1801 taccatagga tttcagttta cagggtaccc gctcaaaatt tccatgctaa ctcggagttt
     1861 ttattttggc aacgatacgt aaacccagac acaagatttg ccggactgtc ggctactgcc
     1921 tttacggcag tcctaaatgc ccttccgtca aaaggccaac gcctcctggg caccatgcag
     1981 atacacgcaa aaagaagaag aaatcgcctt atggggaaca gctgctcgaa aagcaaaagc
     2041 tgcgtctgac ctatggtttg atggaaaagc aattctacca cacgttcgaa cgtgcatcca
     2101 gaatgaaagg caataagtca gacaacttca tgatgctact cgaaggccgc ctgatgactc
     2161 tggtttacag actcggactg gctcgcacca ttttcgacgc acgccagctc attcgccacg
     2221 ggcatgtatc tgtagacgga caacgtgtag acgtaccaag cttcagtgtc tcgcctggtc
     2281 agatcattgg actgactccc ggctctacgg aactggatcg agtgaaaagc gctctggaaa
     2341 cacgtgcatc tgtgagttcg cccactccat acctggaggt gcagtctgac ggtgtatctg
     2401 gcaagtttct aggaattcaa agtgtaaccg atattccggt tacgcgcatc aacgtacaga
     2461 agatcgttga gttctacagc aagtaagctg ctgtggaatc gccggctcta gccggcttaa
     2521 ggcaaacaag caggcctttc aggcctgctt ttcaagtagc attcccgaaa tctcacgcat
     2581 tctctgtgcg aaatccagaa cctttctgga tggaatttcg gatatgattt cgccggtcat
     2641 gtaattgaac agcgtcacct gaactgtgcc atactcagga ttcaccctgt attgaaacct
     2701 ggtgtgctct ttgtcctcat ggaacaggtt gagtgcctgc accgcagaag caattttgtg
     2761 aacgttaggc tctgttgcct cggtttgctg cgccgcaacc gcagcagctt cattctgtct
     2821 cacggctgtc tgatccccgt gaggtcgtgt gggtgtgggg ctatgcccga ctatgctgat
     2881 agcatccatt ctcgatctcc tgggacatcc ctgtccctag attgcaaatc ggtaactgga
     2941 tgaagtacct taacagtacg ttaagaaaaa agattaaagt ttccggtcag aagatccgag
     3001 ggaagtcact ttttcagggc atttttcatg cgcatgagct taccgcgcag acgcatgatt
     3061 gctttggtgt ggagctgaga aattcggctt tctgtgactt caaggacttc ccctatttct
     3121 ttcagggtga ggtcttcata atagtaaaga actataactt tcttttcttt gtccggcaaa
     3181 tgacggatgg cttccacgat tacgcttttg atttcctctc tttctatgag tgaatcaggg
     3241 ttcaggtttg cgggagattc aagggtctcc atgaaggaga cttcgtcgtt atcgtctcca
     3301 agataccaga tatcgttgag ggatacaatg cttgtgcctg agatctttgt gagaagagac
     3361 tgaagctcat ctactgaaat gcccagctct tttgcaatat cttcgtcttc tactgtgtgc
     3421 ccgagtttgt tttcgagcat tgcgattgtt tcttctacct ggcgagcttt ctgacgaata
     3481 gaacgaggaa tccagtcaat gctgcgaagc tcatcgaaga tggctccgcg aatacgcgtc
     3541 atcgcatacg ttttgaattt gatgtcacga tccgggtcga acttttcaat tgcatccagg
     3601 agtccgaaag aaccatagct taccagatca tcgaactcaa cgttttgcgg catgccaatg
     3661 gcgactttgc ccgcaacgta tttcacaagg ggagcatact tttccgcgag gaaatcgcgg
     3721 atctcacggt ttgcttcttt gcgataacga cgccagagct cggtttcgtc tttgtccttg
     3781 tatttatccc agagtctgga catattacgt caccatgcag tgtgttttgt gggttagcaa
     3841 gcaaaacact gtttttactt ttcatccttc gcaagaagtg tgcggattgc tgccgccatt
     3901 agcttcggtt cattcttgat cttcactttg ttgaccagaa tgtgatcgcc gtatgccgca
     3961 ggagctgccg acggagtagg catgctacct gtggacgtgg atacttgcgg aggtgaatcg
     4021 ctttcgtcaa cggatccgcc agaatcgcct gcactgagcg gcg
//
LOCUS       DAORFF010000360         4055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13286_length_4055_cov_3.788000, whole genome shotgun sequence.
ACCESSION   DAORFF010000360 DAORFF010000000
VERSION     DAORFF010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13286_length_4055_cov_3.788000"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKK76_13525"
     CDS             complement(<1..514)
                     /locus_tag="PKK76_13525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNL69547.1"
                     /translation="MRASNRTIAIAAVSITAAVTAFKFFFAIQLPLHPDEAYYWDWSR
                     HLDWSYYDQGPGVAFYIRIFTSVLGNTIPALKLAALSAGLIAHILLFFAARRAGVSAA
                     SSLLFVCCTILVPGFFSGSFLIMHDSVLILFWCAAFLFMLRWIETDSSADLFALFACM
                     ALGFLGKHTMV"
     gene            complement(501..1802)
                     /locus_tag="PKK76_13530"
     CDS             complement(501..1802)
                     /locus_tag="PKK76_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL69548.1"
                     /translation="MSKKASWKEIFGWAMFDFANSSYTTVIITVIWGDLFIRLVVGDG
                     PDFRRGNLAFGIALFISYIFCVITGPIFGAIMDFSAAKKKFLFMSYMLTVVSTCSLFL
                     VSPGMEVLGVVLLIISNFGFAAGESFVASFLPSLGEKEELGKISGIAWGIGYLGGLVS
                     VILVGLLGSVEIENFERLRYVGPMTGIFFMVAAIPTFLWLREPGVAHGLPQGQSYLGV
                     GFARLRQTLSEVKDFRDLGIFFVSLFFASAGLSIVISFAFTYGAQVIHWTDATKTMMF
                     VITQFTALFGALGFGYLQDKIGAKITYMMTLVLWIIAIICIFLTDSIAASLGVKPEQA
                     FLIVGCLAGAGLGATQSAGRALVGVFSPESKAGEFFGLWGLIGKLAAAIGVFGVAALQ
                     TQLGLRLAILFCALLFVAGLLFVIPVNEKRGQAAAEKHEGE"
     gene            complement(1858..2223)
                     /locus_tag="PKK76_13535"
     CDS             complement(1858..2223)
                     /locus_tag="PKK76_13535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL69549.1"
                     /translation="MHPWIPAGGVEFTRKDRGPCSVFHITGDVDLYNSDRLKDALLQH
                     LDSAGKSIILDLTHTDYLDSTGIGAIVAAWNKAKVINQQVMLSSVSESIRRVLSVMQI
                     DAHIQIFEHTDAALASLRR"
     gene            2389..3636
                     /locus_tag="PKK76_13540"
     CDS             2389..3636
                     /locus_tag="PKK76_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNL69550.1"
                     /translation="MQIMLSGGGALDFYHMMASASSNYRLAPFFQPIVSIGDGSIYGY
                     EVLGRFVDESGHFTSMGPYFADPTVPGHEKLIRSRKVRELAFLHARDVNYQGRLFINV
                     KPSWLMQVFRAGSEIPTLRLLEELGLNPRNVVIEITEDEFEGDLVILDSVISRYREAG
                     CKIAIDDFTFHNFDRLIHLKPDIVKIDIRLVKKSVEKKEYQKLISSISDFAQEIGISV
                     LFEGVEFEEELVNSIDAGGSYIQGYFFSQPEPDLQIADTYRDRIRAVLDSVIQNQRQE
                     NYSLLSLEFHLNELMEEMLKKISFEGDLDEALNRLLGSLPSACYRVYVCDQFGNQRSS
                     NFLRGSNAVFTRDDHFRSRNWGWRPYFFHNLARMDRFRRGFMSNRYIDQETKKETYTY
                     SHPLYGNLFIFLDFTRGSFSSPE"
     gene            complement(3605..>4055)
                     /locus_tag="PKK76_13545"
     CDS             complement(3605..>4055)
                     /locus_tag="PKK76_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNL69551.1"
                     /translation="AILEEEPRSATAIAVDKVRTLNFNRANFDTLMKSNPQLAFRLLV
                     IFSNRINDARRRLQILLLDDTTSKVVDVFLMLAEKDAHYGQVKSMVFNTTVDDIASWC
                     GQPIDEVQKIINTFVRQGKVELFADRIVVSNINDFNRLIQAKRKSLL"
BASE COUNT         1070 a          980 c         1025 g          980 t
ORIGIN      
        1 aaaccatggt gtgcttcccg aggaagccca gggccatgca cgcaaataag gcgaatagat
       61 ctgcggacga atctgtttcg atccatctga gcatgaacaa gaatgccgcg caccagaaca
      121 ggatgagaac tgaatcgtgc atgatcagga atgatccgga aaaaaagccg ggcaccagaa
      181 tggtgcagca tacaaacaag agagacgagg ccgctgaaac tccggcgcgt cgcgctgcaa
      241 agaacaagag aatgtgcgct atgagccccg cagagagcgc cgccagtttc agtgccggta
      301 tggtgtttcc tagaactgaa gtgaagattc ggatgtaaaa ggcaactccc ggaccttgat
      361 cgtagtagga ccagtccaga tgcctggacc aatcccagta gtatgcttca tctggatgga
      421 gaggaagctg gattgcgaag aagaatttga aggcggtcac tgcagccgtg atggacacgg
      481 ctgcaattgc tatggttcga ttactcgccc tcatgttttt cagcagcagc ctggccgcgc
      541 ttttcgttca caggaattac gaacagcagt cccgccacaa acaacagagc acagaataga
      601 atcgcgaggc gaaggccaag ctgtgtctgg agtgcggcca cgccaaatac tccaatggca
      661 gctgcgagtt ttccaatcag tccccacagt ccaaagaatt cgccggcctt tgattccggt
      721 gaaaatacgc caacgagtgc gcggccggca gactgcgtcg cacccaggcc agcacctgcc
      781 agacatccta caatcaaaaa cgcctgttcc ggttttactc cgagggatgc cgcgatcgaa
      841 tcagtgagga agatgcagat gattgcaatg atccagagga ccagagtcat catgtatgtg
      901 atctttgcac caatcttgtc ttgcagataa ccaaatccga gtgcaccaaa cagtgctgtg
      961 aattgagtga tcacgaacat catggtcttg gttgcgtctg tccagtgaat cacctgcgct
     1021 ccgtatgtga atgcaaagct gatgacaatc gagagcccgg cggacgcgaa gaagagagaa
     1081 acaaagaaga tgcccagatc gcggaagtct ttaacttcgc tcagagtctg gcgaagtcgc
     1141 gcgaatccaa ctccaagata actttgtccc tgcggaagcc cgtgtgcgac gcctggttcg
     1201 cggagccaca ggaaggttgg aatagcggcg accatgaaga aaatccctgt cataggaccg
     1261 acatagcgca gcctttcaaa gttctcgatt tctacacttc ccagtagacc aactagaatc
     1321 acagagacga gtccgcccag atacccaatg ccccaggcaa tcccggagat tttcccgagt
     1381 tcttcctttt caccaaggct cggaaggaag ctggccacga atgattcgcc ggcggcgaat
     1441 ccgaagttgg agatgatcaa cagtaccacg cccagcactt ccatgccagg cgaaaccagg
     1501 aacaaggagc aggtggaaac cactgtgagc atataactca taaacagaaa tttctttttg
     1561 gccgcactga aatccatgat ggccccgaag atgggcccgg tgatgacgca gaagatatag
     1621 ctgatgaaga gtgcgatgcc aaatgcaagg tttccccttc taaaatcagg cccatctccc
     1681 acaacgaggc gaatgaagag atccccccat atgactgtaa tgatgacggt agtgtaggac
     1741 gagttcgcaa agtcgaacat tgcccagccg aatatctctt tccaggacgc ctttttgctc
     1801 atgtattgct ccggcgaact ttatgaaagt gagcgcagtt ttgtgaagca caaaacttca
     1861 tctgcgaaga gaagcaaggg ccgcgtccgt gtgttcgaag atctgaatat gagcatcaat
     1921 ctgcattaca gaaagaacac ggcggatgga ctcgcttacg ctggaaagca taacctgctg
     1981 gttgatcact ttagctttgt tccaggcagc gacaattgct ccgatcccgg ttgagtccaa
     2041 ataatccgtg tgggtcaggt ccaggatgat gctctttcct gcagagtcca gatgttgcag
     2101 cagcgcatct tttaagcgat cggaattgta gagatccaca tcccctgtga tgtggaatac
     2161 actgcaaggg cccctatcct ttcgtgtgaa ttccacgcct ccagcgggta tccaaggatg
     2221 cataccctca agcaattttt tttaaaccga tgcgcctcga tacatgtcgc ccaaactttg
     2281 tgcagttaga gtagtttctt tccctggttt tttggaatcg acgtgcccgc aacactgtga
     2341 aacatagtgt gatcgttgtc gacatgatcg tggcacgata cgtgcataat gcaaattatg
     2401 ctaagcgggg gtggtgcgct ggacttctat cacatgatgg cgagtgccag ttcgaattat
     2461 cgacttgcac ctttctttca accgattgtc tcgattggag acggatccat ttacgggtac
     2521 gaagtgctcg ggcgtttcgt ggatgagtca ggtcacttca ccagtatggg tccttatttc
     2581 gcggacccaa cggtaccggg tcatgaaaag ctcattcgat ctcgcaaagt cagagagctt
     2641 gcatttctgc atgcgcgaga tgtgaactat cagggcaggc tgtttatcaa cgtgaagcct
     2701 tcctggttga tgcaggtctt tcgcgcagga tctgaaatcc caactcttcg ccttctcgag
     2761 gaactcgggc tcaatccgcg caacgtagtc attgaaatca ccgaagatga atttgaagga
     2821 gacctggtga ttctggattc tgtgatttca cgctatcgcg aagcaggatg caagatcgca
     2881 atcgacgatt tcacttttca caactttgac agactcattc atctcaagcc agacatagtc
     2941 aaaatagata ttcgcctggt gaagaagagc gtagagaaaa aagaatatca aaagctgatt
     3001 tcttctattt ctgatttcgc gcaagagatc ggaatatctg tccttttcga aggcgttgag
     3061 tttgaagaag aactagtcaa ctcgatcgat gcgggtggta gctacattca gggatatttc
     3121 ttttcgcaac ctgaaccaga tttgcaaatt gcagatacat atcgcgacag aattcgtgcc
     3181 gtgctcgact cagtgatcca aaaccagcgt caggagaact acagcctctt gtctcttgag
     3241 tttcatttga acgaactcat ggaagaaatg ctcaagaaga tttcgtttga aggggatctg
     3301 gacgaagcgc tcaatcgcct tctgggaagt cttccttctg cgtgctatcg tgtctatgta
     3361 tgcgatcagt ttggaaatca aaggtcttcg aatttcctgc gaggttcgaa tgcagtcttc
     3421 accagagacg atcactttcg ctcgagaaac tggggttggc ggccttactt tttccataat
     3481 ctggcgcgaa tggatcgatt caggcgtgga tttatgtcca atcgttacat agatcaggaa
     3541 acaaaaaaag aaacgtacac gtattcccat ccattgtacg ggaatctctt catctttctg
     3601 gattttacaa gaggctcttt ctcttcgcct gaatgagccg gttgaagtcg ttgatattgc
     3661 tgacgacgat tctatccgcg aaaagttcta ctttgccctg acgaacaaat gtattgatga
     3721 tcttctgtac ttcgtcgatg ggttgaccac accagctggc aatatcgtcc acagtcgtgt
     3781 tgaacaccat ggacttgacc tgaccatagt gtgcgtcttt ttcagcgagc atcaggaata
     3841 cgtccaccac cttcgatgtg gtgtcatcga gcagaaggat ttgaagtcgt ctgcgcgcat
     3901 cgttgatgcg atttgaaaag atgacaagca gtctgaatgc aagttgcggg ttggacttca
     3961 tgagagtgtc aaaattcgca cggttgaaat tgagtgtgcg aactttgtct actgcaatgg
     4021 cagtggcgct gcgcggttct tcttccagaa ttgcc
//
LOCUS       DAORFF010000361         4002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13568_length_4002_cov_4.051685, whole genome shotgun sequence.
ACCESSION   DAORFF010000361 DAORFF010000000
VERSION     DAORFF010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13568_length_4002_cov_4.051685"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1749)
                     /locus_tag="PKK76_13550"
     CDS             complement(<1..1749)
                     /locus_tag="PKK76_13550"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNL69552.1"
                     /translation="MNRFLGFFPQRPVLCLMLTLCGCFLGAISLLHLPVGLMPNLANP
                     GITIVTRYPGVSAEKIHQMITIPIERSVSDLGNIEEILSVSSEGESKINLLFSNEADI
                     KIKMVEASERIDLASARFPRDVNAPYIVQYDPSDRPVFVVSFSSSHMDLKALREHIDR
                     KVKLKFERVEGVSEVFVGGGFEREIQIAVDPGKLSTSGTTISGVQSSIAEANTYVPGG
                     RIGIPRETSLYTDARLTSTTSLAEVPVRGQKGSWLPLDRLAHVTDSFRDPDSVARTNG
                     EERVTIFIQKSGKANTLSLTEACETIVSGLGGEEFETHIVHNQGEHIRRAIRRVSLEC
                     IIGGIAALIVLQAFLQKRSISLLIGAAIPACILTTFFLMFLLGIDLNVMTLAGLALGA
                     GMLVDNSVVVCESVERLAAAGADPIDAILRGAARVMPEIISSTLCTIVVFLPLLFTEP
                     DTRRMYSGLALTVSASLVLSLVFALTVMPGFLYATSASWTRPDEGSPNRLARVMRNSL
                     GQAKQMYTLLLLRVFRRPKPLIVAAILGDACNYQITNSAASNTTSVYWITSGTGTFSN
                     STDLHPFYYPSTTDYAL"
     gene            complement(1746..>4002)
                     /locus_tag="PKK76_13555"
     CDS             complement(1746..>4002)
                     /locus_tag="PKK76_13555"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNL69553.1"
                     /translation="KLEIVQDKSDFVLSAIANVRTEALSGGIIAVIVLFLFLKDVWPS
                     LIISITIPISIITTFACMYIKGLSLNIMSLGGLALGVGMLLDSAIVVIESITLEREQN
                     PDDSLIDTAVRGTQQVVGSVISAAGTTIVVFVPIIFVPGIAGQVFKDFAFTITFSLLS
                     SLLCSLTLVPVLFSLPVYKVTWMRRAFEKINHISKPAFGYAEKFTSLASRLYDRSIDY
                     SLARPARILLISLGVGVVGIALLCFLPRSLFPDVDQGVVEAEVEMPPGTTLAENEALQ
                     QKIHGFLSKNNLAAHAITTLGFESDDLASQVKGIKHPNYSRSIIYIDRDRMTSREFNE
                     TLSGGLSNFANIKTTFRIKGEALQELLGETSPVIVAEVEHRSRTRAREAALALRESIQ
                     KTKGIVNISSTAGATEPEVKVFVDREKIAAQGLTIESIARTIHSAVNGTIATGYREGD
                     HEIGVRLRLREEDRNSLDHVRRLLVPVGDGKQMEVGTFIRPQEGTGHSAVLRENQRRV
                     EQIRVEYEKSERKTVFAELEQAISNTRASYAKEPDENRPQLRLREDNEETSESLAGLV
                     YAFAISAVLIYMLLAGQFESLIHPITLALIIPLMLFGVSTALLITGHGLNISSAIGII
                     LLAGIVVDSATVLYEYIEQQRGDAGHDGSDIALLPEILKKCGHARIRAILLTSLTNML
                     GSLPSAGLASSGNLQAPMAVAIVGGLIVATVLTLVAFPTMFLVVETFRHRVRQNGTLL
                     AIKSLTARPAA"
BASE COUNT          990 a          970 c         1062 g          980 t
ORIGIN      
        1 aagagcataa tcagttgtgc tggggtaata gaacggatga agatcggttg aattgctgaa
       61 agtacccgtt cccgaagtaa tccagtaaac tgatgttgta ttgcttgcag ccgaattggt
      121 aatctggtaa ttgcaggcat cgcccagaat ggcggcgaca atcagcggtt ttgggcggcg
      181 aaataccctt aaaagcaaaa gagtgtacat ttgctttgcc tgacccaaac tatttcgcat
      241 aactctggca agacgatttg gacttccttc atcagggcgc gtccaggaag cagaggttgc
      301 gtaaagaaat cctggcatga ccgtgagcgc gaaaactaga gaaagcacga gagaagcgga
      361 cacagtcaga gccagaccgg agtacattcg tcttgtgtct ggttccgtaa atagaagggg
      421 aagaaagacc acaatggtgc ataacgtgga ggagatgatt tcaggcatca ctctggccgc
      481 cccgcgcagg atcgcgtcga tcggatctgc tcctgcagcc gcgagtcttt ccacggattc
      541 gcatacaact acagagttat ccacaagcat gcctgcaccc agtgcgaggc ctgccagtgt
      601 cattacattg aggtcaatac ccaggaggaa catgaggaag aaggtcgtca gaatgcaggc
      661 aggtatggcg gctccaatga gtagcgaaat gcttctcttt tgcagaaagg cctgcagcac
      721 aatcaaggct gcgattccac cgatgatgca ttccagagaa acccggcgaa tggcgcgccg
      781 gatgtgttcg ccctggttgt gaacaatgtg agtttcaaat tcttctccgc ccaggcctga
      841 cacgattgtt tcgcacgctt ccgtaagcga aagtgtgttt gccttccccg atttctgaat
      901 gaagattgtg acgcgctcct ccccgtttgt tctggcaaca gaatcaggat cgcgaaaaga
      961 gtctgtcacg tgggcgagtc gatcgagggg aagccacgaa cctttctggc cgcgcactgg
     1021 aacttccgca agagatgtcg tagacgtcag gcgagcatcc gtgtacagcg aagtctcgcg
     1081 agggatgcca attcttcctc ctggaacata ggtgttcgct tctgcgatgg acgattgtac
     1141 accagagatg gttgtgccgg atgtggatag ttttcctgga tcaactgcta tctggatttc
     1201 gcgttcgaag ccgcctccga cgaacacttc cgatactcct tccactcttt cgaatttgag
     1261 tttcactttt ctgtctatgt gttcgcgaag cgccttcaaa tccatgtgcg aactggagaa
     1321 ggaaacaaca aacacgggtc gatcggaggg atcgtactgc acaatgtagg gcgcgtttac
     1381 atcacgagga aaccttgcgg atgcaagatc aatgcgctcg cttgcttcta ccattttgat
     1441 tttgatgtcc gcttcgttgc tgaatagcag gttgatcttt gattctcctt cgcttgatac
     1501 ggacaggatt tcctcgatgt tcccaagatc gcttacgctt ctctcaatgg gaatggtgat
     1561 catttgatgg atcttctctg cggaaacacc ggggtatcgc gtaacaatgg ttatgccagg
     1621 attcgcaaga tttggcatga ggccgacagg caaatggagg agcgaaatgg cgcccagaaa
     1681 gcagccgcac agcgtgagca tcagacacaa cacaggcctc tgtggaaaga atccgagaaa
     1741 gcggttcatg cagcgggcct tgcagtcagg gatttgatgg ccagaagagt tccgttctgc
     1801 ctgaccctgt ggcggaacgt ctctactaca agaaacatgg tcgggaatgc gaccagagtg
     1861 agcactgtgg ctacgatcaa tccgcctacg atggccactg ccataggcgc ctggagattc
     1921 ccacttgagg cgagtcccgc agatggaaga gagccaagca tgttggtaag gcttgtgagt
     1981 agaatcgcac ggatgcgagc gtgtccgcat ttctttagaa tttcaggcaa aagcgcgatg
     2041 tctgatccgt cgtgtccggc gtcacctcgc tgctgttcta tgtactcata gagaacagtg
     2101 gctgaatcca caacgatccc tgccagaaga atgatgccaa tggcagaaga gatattgagc
     2161 ccgtgtcctg tgatgagaag tgctgtagac acaccgaata gcatcagtgg aatgatgagt
     2221 gccagagtga ttggatgaat gagtgactca aattgacctg cgagcaacat gtagatgagc
     2281 acagctgaaa ttgcaaacgc atacactaga cctgccaggg attcgcttgt ttcttcgttg
     2341 tcttcgcgta gccgcagttg cggacggttt tcatctggct ctttcgcata ggatgcgcgg
     2401 gtatttgaga tggcctgttc cagctctgcg aagactgtct ttcgttcgct cttttcatat
     2461 tcgacccgga tttgctctac acgtctctga ttctcgcgaa gaactgcaga atgacccgtg
     2521 ccttcttgag ggcggatgaa agtgcccact tccatttgct tgccatcgcc cacaggcaca
     2581 agcaagcgtc tcacatggtc gaggctgttt ctgtcctctt cgcgaagtct gagcctgaca
     2641 ccgatttcgt ggtccccttc gcggtagccg gttgcgatgg tgccgttgac cgcgctatgg
     2701 atggtgcgtg cgatcgattc gatggtcagc ccctgggctg ctattttctc gcgatccaca
     2761 aacaccttta cctcaggttc cgttgcaccg gcggtagatg agatgtttac aattcctttt
     2821 gttttttgaa tgctttcgcg cagagccagg gccgcctcgc gagcgcgggt tcgcgaccta
     2881 tgttctactt ctgcgacgat cacgggagat gtttctccga gcagttcttg cagtgcttct
     2941 cctttgatcc tgaatgttgt cttgatattt gcgaaattgg aaagaccgcc ggacaacgtc
     3001 tcattgaatt ctcgagacgt catgcgatct cgatcaatgt agatgatgct gcgagagtag
     3061 ttcggatgct tgattccttt tacctggctg gcaaggtcat cggattcaaa tccgagagta
     3121 gtgatcgcat gagcggccag attattttta ctcagaaagc cgtgaatctt ctgctgcaat
     3181 gcttcatttt ctgccagagt agtgcctgga ggcatttcca cttctgcttc cacaacaccc
     3241 tgatctacgt ccgggaaaag gcttcgcgga agaaaacata ggagtgcgat tccaacaacg
     3301 cccactccga gtgagatgag taaaatccgc gcaggtcttg caagggaata gtcaatagag
     3361 cgatcataga gacgcgaagc aagagatgtg aatttctctg cgtaaccaaa ggcaggcttt
     3421 gagatgtgat tgatcttttc aaatgctcgc ctcatccacg ttactttgta cactggcaga
     3481 gaaaaaagaa cgggaacgag agtcagagaa cagagaaggg agctcagaag agagaatgtg
     3541 atcgtgaaag cgaaatcctt gaatacctgt cctgcaatcc ctggaacgaa aataatggga
     3601 acaaatacca cgatcgttgt gcctgcagca gatatgacag agcccacaac ttgctgagtt
     3661 ccccgcactg cagtatctat cagtgaatca tctggatttt gttctcgttc gagtgtgatc
     3721 gattcgatca ctacaatcgc actgtcgagc agcatgccca ccccgagtgc caggcctccc
     3781 aggctcatga tgttcagcga aagccctttg atgtacatgc atgcgaacgt ggtgataatt
     3841 gaaattggaa tcgtgatcga aatgatgaga gaaggccaga catcctttag aaacaaaaac
     3901 agaacgatca ctgcgatgat gcctccggag agcgcctctg ttctcacgtt tgcaatggca
     3961 gacagaacaa aatcggattt atcctgaaca atttccagct tc
//
LOCUS       DAORFF010000362         4001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13579_length_4001_cov_4.221237, whole genome shotgun sequence.
ACCESSION   DAORFF010000362 DAORFF010000000
VERSION     DAORFF010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13579_length_4001_cov_4.221237"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PKK76_13560"
     CDS             complement(<1..489)
                     /locus_tag="PKK76_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69554.1"
                     /translation="MRFPFAVKLALSISLLAVSVTSLSVVFLYTDTRSMVIRLMAGRL
                     KDVGRTAAYMLESQDREGMKKMATVVRSKIKITAELRKTIADAKVGDTIPSPLTQEEI
                     DKIIQSPDYLEMVQFLRQVKAGSRESVDYPVHLSQVFETDPPQIRYSYVMISLPEYPA
                     HDV"
     gene            complement(493..1245)
                     /gene="gntF"
                     /locus_tag="PKK76_13565"
     CDS             complement(493..1245)
                     /gene="gntF"
                     /locus_tag="PKK76_13565"
                     /inference="COORDINATES: protein motif:HMM:NF013405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis pre-guanitoxin forming
                     N-methyltransferase GntF"
                     /protein_id="HNL69555.1"
                     /translation="MSSEITLDYNEFSPQDYLNEYYTEVSDENESLLQFFHDTYSKLG
                     PVDSMLEVGGGPTIYQIISASRVAKHIDFSEYLPANLAMVRAWLDQKPGSFNWDIYFK
                     RVLEIEGRPASRENIDALAGTVRGAVRRLTQYDVFREPPLVEGEPLYDIIASNFCIEG
                     IQTDRQVFERQFGRMTSYLRPGGLLALCMVKNATYYRVGKRRFPVVPVNETIMENVLK
                     NAGLTLTHIQSIKAVDPDQGYEGMLMIRATRG"
     gene            1359..2813
                     /locus_tag="PKK76_13570"
     CDS             1359..2813
                     /locus_tag="PKK76_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNL69556.1"
                     /translation="MFLDQNRAPLLDALIAHHAEGVHPFHVPGHKHGVGARDLLSLFG
                     QTTLSFDLNAMHDLDDACNPVGVIDEAQRLAAHLFGADHAHFLVNGNTQGIHSMMLSA
                     VGPGEQIVLPRNCHRSAITGLILSGGLPIYVPVEIDAHLGVATGPTVQSVRATLEKNP
                     FVCALFVVNPSYYGFATDIEGMVRAAHDHRAAALVDEAHGTHLSFHPDFPANAMSVGA
                     DMSAVSMHKTGGSLTQSAILLSKGERVPHDILKESIDLIRSTSASYLLMVSLDTARRS
                     LAQDAPGQFERVLQLARFARTEINLIEGLYSPGKEICRPDSSVFNYDESKLLIHCASA
                     GFTGFEMERLLRDRFSIQVELADMNNVLALITIGTREEDILHLVGSLKTIVLGNNRKH
                     SRPTVPPPLMPDVISSPRQAFYSPKKFMPLSGSAGQISGEMLMSYPPGIPIICPGERI
                     TQDIIDYVRVLKEQGSLLQGTADPLAENIRVLGA"
     gene            2810..>4001
                     /locus_tag="PKK76_13575"
     CDS             2810..>4001
                     /locus_tag="PKK76_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69557.1"
                     /translation="MKFLSRASGITEKEWPRARSMMLLCFAIMAALAFGRAARDAFFI
                     KNAGADNLPIVYLLNGILVAGIAAVYSRVVHRLSLSRFMGLLCAAFVLAIVVLRLLIS
                     SGNVVMPYAVWSLAEVYNILMLMHFWTYANKVFDPREGKRMFPIVGAAGLVGMIVGSS
                     VTPMFVNAFGSNNLLLGWAGMVILAIPPLRLSAAAARKSGVILAGTDDSGETVAENWR
                     SLFQIPLVRTLAMIAFPMWITAWLVDYQFFHALDEVYKEQDSLAGFLGVFNGLSSLAG
                     LILQFFVTGRLLQTLGVGGTSILYPGVVAASAVGLCARSMMPVVPGKVYEPRSILGIA
                     GKFSDETLFGSVHDSAIAVLFSSLPDENRARARALLNGAMEPMATAVAGILLLVLRRF
                     HAPPI"
BASE COUNT          861 a          980 c         1043 g         1117 t
ORIGIN      
        1 cacatcgtgc gccgggtatt ccggcagaga aatcattacg tagctgtagc ggatttgagg
       61 aggatccgtt tcaaaaacct ggctcaggtg aaccggatag tctacgcttt cgcgcgaacc
      121 cgcttttacc tggcgaagga actgaaccat ttccaggtag tccggagatt ggatgatctt
      181 gtctatttct tcctgagtga gagggcttgg aattgtatcg cccactttcg cgtccgcgat
      241 tgtttttctg agttccgctg tgattttgat tttgctgcga acaaccgtgg ccattttttt
      301 catgccttcg cggtcctgcg attcaagcat gtaagccgca gttcttccca cgtctttgag
      361 gcgtccggcc atgagtcgaa tcaccatact gcgtgtgtcc gtgtatagaa acacaacgct
      421 caagctcgta acagaaacag cgagcagtga aatcgaaagt gcaagtttta ctgcgaatgg
      481 aaatctcacg tcttaacccc tcgtcgcacg gatcatcagc attccttcgt aaccctgatc
      541 tggatccacc gctttgatcg actggatatg agtcagagta aggcccgcat tcttcaacac
      601 gttttccata atcgtttcat ttactggtac gactggaaat cgtcttttgc ctacgcgata
      661 atatgtcgca ttcttcacca tgcacagagc caggagaccg cctggcctga gataggatgt
      721 catacgacca aactgtcttt caaaaacttg ccggtctgtc tggatgcctt caatgcaaaa
      781 attggacgcg attatgtcgt ataacggttc gccttccacc agtggaggct cgcgaaacac
      841 gtcgtattga gtcaaccgtc tcactgcgcc gcgaaccgta cctgcgagtg cgtctatgtt
      901 ttcgcgcgaa gcaggtcttc cttcgatttc aagaacgcgt ttgaagtaga tgtcccagtt
      961 gaatgatcct ggtttttggt ctagccaggc gcggaccatg gcaagattcg cgggcagata
     1021 ctctgaaaag tctatgtgtt tggcaactct gctcgcgctg atgatctgat aaatggtcgg
     1081 accgccgccg acttctaaca tgctgtctac tggcccgagc tttgaatagg tatcgtggaa
     1141 gaactgaagc aggctttcgt tttcgtctga aacttctgtg taatactcgt tcaagtagtc
     1201 ttgaggcgag aactcattgt agtctaaagt gatttctgag gacacgtctc agtatcggcc
     1261 agcgcgaggc tcaaaattca attccgaact gcagttctac gcgtgttcag acgattttga
     1321 ctctccgcgt gccgatcttg taccgactgg ttcgacctat gtttctagac caaaacaggg
     1381 ctcctcttct cgacgcgttg atcgcacacc atgcggaagg agtgcatcca tttcacgtgc
     1441 ctggccacaa gcacggagtg ggcgcacgcg atctcctgag tctgttcggg caaaccactc
     1501 tttcgttcga cctgaatgca atgcatgatc tcgacgacgc gtgcaacccg gtgggggtca
     1561 ttgacgaagc acagagactt gcggctcatt tattcggcgc ggatcacgct cactttcttg
     1621 taaacggaaa cactcaggga attcattcga tgatgctttc tgcggttggg ccaggtgagc
     1681 aaattgtact tccgcgaaat tgtcatcgtt ccgcgatcac aggtttgatc ttatctggcg
     1741 gccttcctat ttacgttccc gtggagattg acgctcatct tggtgtggcc acaggtccaa
     1801 cggttcagag cgttcgcgcg actctggaaa aaaatccatt cgtttgcgcg ttgtttgtgg
     1861 taaatccgtc ttactacggt tttgcgacgg acattgaagg catggttcgc gcagcgcacg
     1921 atcatcgggc tgcagctctc gtagacgagg cgcacggcac gcatctttct tttcatccgg
     1981 attttcctgc aaatgccatg agcgtgggtg cagacatgag tgcagtcagc atgcataaaa
     2041 cggggggatc tctcacgcaa agcgcgattc tcctttccaa aggtgaacga gtgccgcatg
     2101 acattctcaa agagtcgatc gatttgattc gatccacaag tgcgtcttat ttgctgatgg
     2161 tttctctgga tactgcgcga agatctcttg cacaggatgc gcccggtcag tttgaacgcg
     2221 tgcttcagct ggctcgtttc gcacgaacag agatcaattt gattgaaggt ctctattcgc
     2281 ccggaaagga aatttgcagg ccggatagtt ctgtcttcaa ctacgacgaa agtaagttgc
     2341 tcattcattg tgcttcagcc gggttcaccg gttttgagat ggagagattg ctgcgagatc
     2401 gcttttcgat tcaggttgag cttgcggaca tgaacaatgt tcttgctctc attacaatcg
     2461 gaacgcgcga ggaagatata ctgcatctgg tcggaagtct gaagacaatc gtgctcggga
     2521 acaatcgaaa gcattcaagg ccaactgtcc ctccgcctct gatgccagat gtaatttctt
     2581 ctccgcgcca ggctttctat tcgccaaaga agttcatgcc tctatctgga tcggctggac
     2641 aaatcagcgg agagatgttg atgtcctacc cacctggcat tcccattatt tgtccaggag
     2701 aaagaatcac tcaggatatt atagattacg ttcgcgtgct taaagaacag ggaagcttgc
     2761 ttcagggtac ggcagatccg cttgcggaga acattcgggt gctgggtgca tgaaatttct
     2821 ttcgcgagct tctggcatta ctgagaaaga atggcctcgt gctcgcagca tgatgctttt
     2881 gtgtttcgca atcatggcag ctcttgcatt cggtcgagct gcgcgagatg catttttcat
     2941 caagaatgcg ggagcggaca atctaccgat cgtctatctt ctcaatggca tcctggttgc
     3001 cgggattgcg gccgtgtatt cgcgcgtcgt gcatcgattg tctctcagta gattcatggg
     3061 tttactgtgt gctgctttcg ttctcgccat cgttgttctc aggctactga tctcatccgg
     3121 caatgttgta atgccatacg cggtctggtc acttgcggaa gtgtacaaca ttttgatgct
     3181 catgcacttc tggacctacg caaacaaggt ttttgatccg cgcgaaggga agcgcatgtt
     3241 cccgatcgta ggtgcagccg gtctggtggg catgatcgtt ggaagttcag taactcccat
     3301 gttcgtaaat gcgtttggta gtaacaatct gctactcggc tgggcgggca tggtgattct
     3361 tgcgattcca ccacttcgcc tttctgcagc agctgcgcga aagagcggag tcattctcgc
     3421 aggcacggac gacagcggcg aaactgtggc cgagaactgg agatcgctgt ttcagattcc
     3481 gctggttcgc acactggcga tgatcgcctt tccaatgtgg atcacagcgt ggctggttga
     3541 ttatcaattc tttcatgctt tagatgaagt gtacaaagag caggattctc tcgctggctt
     3601 tcttggtgtt tttaacgggc tctcttctct tgcaggattg atacttcagt tcttcgtgac
     3661 cggaagacta cttcaaacgc tgggagtggg tggcacttcg attctttacc cgggagtagt
     3721 cgcagcaagt gcagtggggc tttgtgctag aagtatgatg cccgtcgtgc ccggaaaagt
     3781 ttatgagccg cgctccattc ttggcattgc aggaaaattt tcggatgaga ctctctttgg
     3841 gtctgttcat gattctgcca ttgcggtttt gttcagttca ttgccggatg agaatcgtgc
     3901 gagagctcgt gctcttttga acggtgcgat ggagcccatg gccactgctg tggctggtat
     3961 tttgcttctt gtacttagac gatttcatgc tccacccatc g
//
LOCUS       DAORFF010000363         3992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13627_length_3992_cov_3.270003, whole genome shotgun sequence.
ACCESSION   DAORFF010000363 DAORFF010000000
VERSION     DAORFF010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13627_length_3992_cov_3.270003"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PKK76_13580"
     CDS             complement(<1..316)
                     /locus_tag="PKK76_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016750890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69558.1"
                     /translation="MALSQNLVQKQTQKLIITQDLRQSIELLPLSNLELSEKIEKEVL
                     ENPMLEIAEDFEKPENPAEGPAPENQRTEGAEEDIGESFPDFAVREEYAPVEGSVSGA
                     ERS"
     gene            complement(321..1028)
                     /gene="lptB"
                     /locus_tag="PKK76_13585"
     CDS             complement(321..1028)
                     /gene="lptB"
                     /locus_tag="PKK76_13585"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004430733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNL69559.1"
                     /translation="MEDLSKAYNKRRVVDGVSFEICTREIVGLLGPNGAGKTTSFYMA
                     VGLVAPDSGKIYIDNKDVTTMPMYLRAREGIGYLAQEASVFRKLTVAENIESVLETMN
                     LTRSEIVERRDRLIDELHLNKVTNQNGYTLSGGERRRCEIARALATEPDFILLDEPFA
                     GVDPIAVKDIQQLIGHLRERGLGILITDHNVRETLKITDRAYIMYAGEILKSGTAKEL
                     VNDPKIRQIYLGEDFTL"
     gene            complement(1078..2535)
                     /locus_tag="PKK76_13590"
     CDS             complement(1078..2535)
                     /locus_tag="PKK76_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69560.1"
                     /translation="MKRVAIIVAFLISLSASACRAGFIRQNPPVTEEQTNPQKIDPQT
                     EEKPEPQVEPKIENVNNPENIIEEKTQDSSGRLVRVQIYGDELIREEVEVNGIPVKRT
                     TLRGNAVVLHNGVRIQAPLIVIEGGVRGACTGGVVIADPANGMSIYAERGDYDRQAQT
                     VILSGFPRMVSEGKGRKPAVLTSGRMVRKMADSVSVLEGDVRIATEGWTIVGENAVFA
                     DKDKKIDLPGDPMIFGNGQFITGNTLSYFTEERKLVLHGDIVNIRREDRSIVADKKPP
                     TLEQYVRSGERDNGGLDISGPGTLTADEITHDFKDKENVKTIVRGNVLFTREGLRIAA
                     PSLESIGSSATHLIAREGVDSTDHKQNLHITAGKMVYERASRMLKLEQDPKIEFLKKD
                     SVEVTGSLSGALIERNFETKQTIARGNVRLVQSDVTAVGQKAVFQEDTGAVVIEGEPG
                     IEQEKGIIRCEKIVYYPEKKRLLLMNRIRGSLAKD"
     gene            complement(2532..3077)
                     /gene="lptC"
                     /locus_tag="PKK76_13595"
     CDS             complement(2532..3077)
                     /gene="lptC"
                     /locus_tag="PKK76_13595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNL69561.1"
                     /translation="MNRFLLLILLLAACQKDPLQDDSLEKGASIRLKNFSRKSFSIDG
                     KEEWDLQAKEAYIFRSGDKESRIVAYGLDFKQFDKGRRTGLIQAERGEIDYKKKVLNL
                     AGTVSFTDDKRTVQSEELKYTMEDKILESTASVLLVEGASTTHCRKGISVNRSTNVQV
                     CKSPAVTRVSRPGDKQGFEDL"
     gene            complement(3079..3918)
                     /gene="kdsA"
                     /locus_tag="PKK76_13600"
     CDS             complement(3079..3918)
                     /gene="kdsA"
                     /locus_tag="PKK76_13600"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNL69562.1"
                     /translation="MLIKEREFLAGKKIGGREPFFLIAGPCVIESRDMLKRVCETMKK
                     LSEELGILYIFKSSFDKANRSSHDSKRGPGIEEGLRDLENIRKEFGVPVLTDVHEREQ
                     VKAVSQVVDVLQIPAFLSRQTDLLQVCAESGKWVNVKKGQFMAPQDAAKIADKIKSCG
                     SDKYLVTERGVSFGYNNLVFDPRSPEIIHEMGIPFVFDGTHSTQLPGAGKESGGDRRF
                     APVLMRAAVASGLEGIFMEVHPDPPSAWSDSSNQYYLDQAPELLRGLYKIDRLVKTQI
                     FAG"
BASE COUNT          872 a         1088 c          895 g         1137 t
ORIGIN      
        1 ctgatctttc cgcgcctgac acgctgccct ccaccggggc atactcttcg cgcactgcaa
       61 aatcaggaaa cgattcgccg atgtcttctt ctgcgccttc cgttctttga ttttctggcg
      121 ccggtccctc tgccgggttc tctggctttt caaaatcctc tgcgatctca agcattggat
      181 tctcgaggac ttccttttca atcttctcag acaattcgag attggacagg ggaagcagtt
      241 caatcgactg acgcagatcc tgagtgatga tcagtttttg cgtctgcttt tgaactaaat
      301 tctgcgaaag agccataggt ttaaagtgtg aagtcctcgc ccagatagat ctggcgaatt
      361 ttcggatcgt tcactagttc cttcgcagtt ccagatttca aaatttcacc ggcatacata
      421 atgtatgctc tgtctgtaat cttcaatgtt tcgcgcacgt tgtgatccgt aatcaagatt
      481 ccaagacccc gctcgcgcaa atgaccgatg agctgctgga tgtctttgac tgcaatcggg
      541 tccacgccag caaaaggctc gtcgagcaaa atgaagtcag gctccgtggc cagagctcgt
      601 gcgatttcgc acctgcgcct ttctccacct gaaagagtgt acccattctg attggtgacc
      661 ttgttcaagt gaagctcatc aatgagtcga tcccttcttt ccacgatctc ggatcgggtg
      721 aggttcatag tttctaacac agattcaata ttttctgcca ctgtcaattt tcgaaacaca
      781 gaagcttcct gcgccaggta tccaatccct tcgcgcgctc gcaggtacat gggcatagtg
      841 gttacgtctt tattgtcgat gtaaattttt ccgctgtccg gtgccacgag acccacagcc
      901 atataaaatg aagttgtctt tcccgccccg ttcgggccga ggagtcctac gatttcgcgt
      961 gtgcagattt cgaaactcac gccatctaca actcttcgct tgttgtatgc cttggaaaga
     1021 tcctccatgc gaaagcgacg cactggttct tccgattgtt ttggtctgcg ctgcacgcta
     1081 gtcttttgca agagatcccc tgatccgatt catgagtaaa aggcgcttct tctctggata
     1141 gtaaacgatt ttttcgcaac gaatgattcc cttctcctgc tcgattccag gctctccttc
     1201 aatcaccaca gcacccgtat cttcctgaaa aacggccttt tggcccaccg cagtcacatc
     1261 actctgcacc agtcgaacgt ttccgcgtgc aatcgtttgt tttgtttcaa aatttcgctc
     1321 aatcagggcg ccggaaaggg atcccgtcac ttccacactg tctttcttca gaaattctat
     1381 cttgggatcc tgctcgagtt tgagcatacg gctggcgcgc tcgtagacca ttttgcctgc
     1441 tgtgatatga agattctgct tgtgatccgt ggaatccaca ccctcccgtg ctatcaaatg
     1501 agtggcagaa ctaccgatgg actcaagaga aggtgcggca atgcgaaggc cttcgcgggt
     1561 gaataacacg ttgcctcgca caatcgtttt tacgttttct ttgtccttga aatcgtgggt
     1621 gatttcgtct gcggtcagag tgcccggccc agaaatatcg agacctccgt tgtccctctc
     1681 tccgcttcgc acgtattgct cgagagtggg cggtttcttg tctgcgacaa tggacctatc
     1741 ttcgcgccgg atatttacaa tatctccgtg cagtacgagc ttgcgctcct ctgtaaaata
     1801 actgagagta tttccagtga tgaactgacc attcccaaag atcataggat ctccgggtag
     1861 atcgatcttc ttatccttgt ctgcgaaaac tgcattctct cctacaatgg tccagccttc
     1921 tgtggcaatg cgcacgtccc cttctagaac agaaacactg tccgccatct tgcgcaccat
     1981 ccggccggaa gtcagcactg ccggttttct tcctttgcct tcactcacca ttctgggaaa
     2041 cccggatagg atcactgtct gtgcctgcct gtcgtagtct cctctttctg cgtaaatgga
     2101 catcccgttt gccggatctg caatgaccac accgcccgtg caagcgcctc taacaccacc
     2161 ttcaattacg atgagagggg cctgtatcct cactccgttg tggagcacta cagcattgcc
     2221 gcgtaacgtt gttcttttga cgggaatgcc gttgacttcc acttcttcgc gaatcagttc
     2281 gtctccgtag atttgaactc gtaccaacct tccggaagaa tcctgagtct tctcttcgat
     2341 gatattttcc gggttgttga cgttttctat ttttggctct acctggggct caggcttttc
     2401 ctctgtctgt gggtcaattt tctgaggatt ggtctgttcc tcggtcactg gcgggttctg
     2461 tcgaatgaat ccagcgcggc atgcagatgc agaaaggctg atgaggaatg caacgatgat
     2521 ggcgacgcgt ttcatagatc ttcgaaacct tgcttatcgc ccggcctgga aacgcgagtc
     2581 actgcaggag atttgcaaac ctgaacattg gtagaacgat tcactgagat tccttttctg
     2641 cagtgcgtgg tcgaggctcc ttctactaga agcacgcttg cggtactttc gagaatctta
     2701 tcttccatag tgtacttcag ctcttccgat tgcaccgttc ttttatcatc cgtaaagctt
     2761 actgtgccgg ccaggttgag aactttcttt ttatagtcga tttctcccct ttctgcttga
     2821 atcaaaccgg ttcgtctgcc tttgtcaaac tgtttgaaat cgagcccata ggccacgatg
     2881 cgcgattctt tgtccccgga gcgaaagata tatgcctctt ttgcctgaag gtcccattcc
     2941 tctttgccat ctatactgaa agacttgcgg gaaaaattct tgagccgaat gcttgcacct
     3001 ttctccagtg agtcatcctg aagcggatcc ttttgacagg ctgcgagaag cagaatcaga
     3061 aggagaaatc ggttcatatc agccggcgaa gatctgcgtc ttgacgagtc tgtcgatttt
     3121 gtaaagacca cgcagcaatt caggcgcctg atccaggtaa tactgattgg aagagtcgga
     3181 ccacgcgctc ggaggatctg gatgcacttc catgaaaatt ccctcgagtc ctgaagcaac
     3241 tgcagcgcgc ataagcacag gtgcaaatct acgatcccct ccactctcct ttccggctcc
     3301 gggaagttgc gtactgtgag tcccatcgaa tacaaatgga atacccatct cgtgaatgat
     3361 ttcaggactg cgcggatcga acactagatt gttatacccg aaagaaaccc cgcgttccgt
     3421 cacaaggtac ttgtctgatc cgcaggattt gattttgtcc gcaatcttcg ctgcatcctg
     3481 cggtgccatg aactgtccct tcttaacgtt cacccacttt ccactttccg cgcacacctg
     3541 caaaaggtct gtttgcctcg aaagaaaagc aggaatctga agcacatcta caacctgaga
     3601 gactgccttg acctgttcgc gctcatgcac atctgtgagc actggaacac cgaattcctt
     3661 gcgaatgttt tccagatcgc ggagcccttc ttcaattcca ggcccacgtt ttgaatcatg
     3721 cgatgatctg tttgctttgt caaagctgct cttgaaaata taaagaatcc caagttcctc
     3781 cgaaagcttc ttcatggttt cgcacactct tttgagcatg tctctgcttt cgatgacgca
     3841 cgggcctgca atcaagaaga aaggttctct tcctccaatc ttctttcccg ccaggaattc
     3901 tctttctttg atcaacatag ctacctcgcc agcttagagg cagcagccac gaaacccgca
     3961 aacagagggt ggcatgcaat tggtttagat tg
//
LOCUS       DAORFF010000364         3984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13679_length_3984_cov_3.425554, whole genome shotgun sequence.
ACCESSION   DAORFF010000364 DAORFF010000000
VERSION     DAORFF010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13679_length_3984_cov_3.425554"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /gene="carB"
                     /locus_tag="PKK76_13605"
     CDS             complement(<1..480)
                     /gene="carB"
                     /locus_tag="PKK76_13605"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771103.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HNL69563.1"
                     /translation="MPRRTDLRSILIPGSGPIIIGQACEFDYSGTQACKVLRKEGYRT
                     ILVNSNPATIMTDPGLADATYLEPLTPEFLMQIIEKERPDAILPTVGGQTALNLAMSL
                     WKSGFLQKMGVEMIGANAEAIHRAEDREEFKRAMIRIGLKVPLSVTCETMQTARDFKQ
                     "
     gene            complement(538..1608)
                     /locus_tag="PKK76_13610"
     CDS             complement(538..1608)
                     /locus_tag="PKK76_13610"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNL69564.1"
                     /translation="MTESNLNHDRDVAEKKGAIGRLVQKGLDSSIEKARQKGQERLTI
                     MLIPHGQEKIFSLQLNYQMIFFLLGILFLVVGLSFYGFYRKAQKELDADRLRSQYGLN
                     FRGAMTLKEISGDLGELNETLYSNLKEIASLTGVPDEEIQELPEISSSYSRANNQLEQ
                     EIIQKSKLGAGRNFLPPVYSLKRLTYAFSDQVRALSTVERLVGQGIGLYSAMPMGRPF
                     TEKEMLALRDTSGFGMRVDPFTNAALEMHAGQDIAGPDGTPVRATGTGEVQSTGYDMG
                     YGNRIVVKHGSAYYSTYSHLQRILVRPGEIVRRGQVIGQMGRTGRVTGSHLHYEVWVG
                     DQRRTDPVPYMCAYDFRSDICR"
     gene            1660..3252
                     /gene="lysS"
                     /locus_tag="PKK76_13615"
     CDS             1660..3252
                     /gene="lysS"
                     /locus_tag="PKK76_13615"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNL69565.1"
                     /translation="MASKEKSNAQKKPQQSPKPAPEAAPEHDASDLFETRLRKLEEAR
                     ASQNPYDISYQRKNTVSEPASVAVDSIAEGTPGSLVVYNVAGRVRAKRLMGKAGFLDL
                     EDETGRLQIYGTEKDASLDFNTFVSLDLGDIAGFEGTLFVTRTGQISLRATKMTLLAK
                     CLRPLPVVKEADGKVFDAFTDKESRYRMRYVDLIVNPHVRKTFLIRSQIVSQIRSFLN
                     AEGYLEVETPMMHPIPGGASAKPFTTHHNALDMELFLRIAPELYLKRLLVGGFSKVYE
                     INRNFRNEGISFKHNPEFTMLELYEAYGDMNSMLDLCERLFVDLAERVIGSTKLPFEE
                     DFIELKRPWERLTYFDAIKKYAKIDLKENMTGEQAVAAAKAAGLRDEELAHADSVWTV
                     AECIFDARVEEKLMQPVFITHHPIAVSPLAKADPANRNFALRFETYIAGRELCNAFSE
                     LNDPIDQKARFQAQVELREKGSEGGGYMDHDYIRALEYGMPPAGGMGIGIDRLVMLFT
                     NSHTIRDVILFPVLRPETAGSD"
     gene            complement(3253..>3984)
                     /locus_tag="PKK76_13620"
     CDS             complement(3253..>3984)
                     /locus_tag="PKK76_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNL69566.1"
                     /translation="MADAVKKISWGKFMNNGQTCIAPDYALVHKDRYAEFIEGMKKTV
                     ASYYGKSEEERKQSPHYARIISSRHHARLSDMVAKSVEQGAKLEFGGITDTTEQYIAP
                     TLLSDVPLSAPVMQEEIFGPILPVIPIDSLEEGLGIVSKLEKPLALYFFSKSNRHIKY
                     VLSETTSGGTCINETLIHFLHPNLPFGGVNFSGSGNSHGFYGFRAFSHERAVLKHHRF
                     APMKLMFPPYTNTVRKLIEFVVKYL"
BASE COUNT          949 a          967 c         1020 g         1048 t
ORIGIN      
        1 ttgtttgaag tccctggcag tctgcatggt ttcgcatgta acgctgagag gaactttcag
       61 accgatccga atcattgctc tcttaaattc ttctctgtct tctgctcggt gaatcgcttc
      121 cgcgttagcg ccgatcattt caacgcccat cttctgtaag aagcctgatt tccagaggct
      181 catggccaga ttcagtgcgg tctgtccacc gacggtggga agaattgcgt ctggcctctc
      241 tttctcgatg atttgcatca ggaattcagg agtcagaggt tcgaggtaag ttgcatctgc
      301 gaggccgggg tccgtcatga tggtcgccgg attagagttc accaggatgg tgcgatagcc
      361 ttctttcctc aggaccttgc aggcctgggt gccactgtaa tcaaactcgc aggcctgccc
      421 aatgatgatt gggccactgc ctggaatcaa tatgcttcgc aagtcagttc ggcgtggcat
      481 ggcaaacgct gacagaattt tgccagggcc tatggtgcca actggaattt caaagtttca
      541 acggcagatg tcgcttctaa agtcgtaggc acacatgtat ggcaccggat ccgttcgcct
      601 ttgatcgccc acccagactt cgtagtgcaa atggcttcct gtgactctcc cggttcgtcc
      661 catctggcct atgacctgac cacgtcttac gatttctccg gggcgaacca ggattcgctg
      721 tagatgcgaa tacgttgaat aataggcact tccgtgtttt actacgatgc gatttccata
      781 tcccatgtcg tatccggtgc tctgcacttc tccagttcct gtggcgcgca ctggagtacc
      841 gtcggggcct gcgatgtcct ggcctgcatg catttccagg gccgcgtttg tgaatggatc
      901 cactctcatt ccaaatccgg acgtgtcgcg aagtgcgagc atttcttttt cagtgaaagg
      961 tcttcccatg ggcatcgcag agtagagtcc gatcccctgg cctacgagtc tttccactgt
     1021 gctgagcgcg cgcacctgat cagagaatgc gtatgtcagt cttttgaggg agtatacggg
     1081 gggcagaaag tttcttcccg cacccagttt tgatttttga atgatttcct gttccagttg
     1141 attgtttgca cgcgaatagg aggaactgat ttcaggaagc tcctgaattt cttcgtccgg
     1201 cacacctgtg aggctagcga tctctttgag attggaataa agagtttcgt ttagttctcc
     1261 gagatctccg gaaatttctt tgagagtcat ggctccgcga aaattcaaac catactgact
     1321 gcggagtcta tctgcatcga gctccttctg tgcttttcta tagaaaccat agaaggaaag
     1381 tcccacgacg aggaagagaa tgcccagcaa aaagaaaatc atctggtagt tcagctgcag
     1441 actgaatatc ttttcctggc cgtgcggaat gagcatgatg gtcagacgtt cctggccctt
     1501 ctgacgcgcc ttttctatag aggaatcgag tcccttctgg acgagccttc caattgcgcc
     1561 ctttttctcc gccacatctc tatcatggtt gaggttcgat tctgtcaaat acgatcctct
     1621 tcgatttgac cggcagtgcg atagagacag atctggtcca tggccagcaa agagaaatca
     1681 aacgcgcaaa agaaaccaca gcagtcgccc aaacctgcac ctgaggctgc gccagagcat
     1741 gatgcgtcgg atctatttga aacaagactc cgcaaactgg aagaagcacg cgcttctcag
     1801 aatccatatg acatttcgta tcagagaaag aacacggtat ctgagcctgc gtccgttgct
     1861 gtggattcga tcgcggaagg aacaccgggc tctctagtcg tttataatgt agcaggtaga
     1921 gttcgcgcga agcgtttgat ggggaaggct ggtttcctgg acctggaaga tgaaacaggg
     1981 aggcttcaga tctacggaac ggaaaaagat gcgtccctgg actttaacac gtttgtttct
     2041 ctggacctgg gagacatcgc aggctttgaa ggcactcttt ttgtaactcg cactggtcag
     2101 atttcgctca gagctacgaa gatgactctt cttgcgaaat gtcttcgtcc gcttcctgta
     2161 gtcaaagagg cagatggaaa agtgttcgat gcatttacgg acaaagaaag ccgttatcgt
     2221 atgcgttatg tggatttgat tgtgaatccg cacgtgcgaa aaacattttt gattcgctca
     2281 caaatcgttt cgcagatccg ttcttttctc aatgcggaag gttatctgga agtggaaaca
     2341 cccatgatgc atccaattcc aggtggtgcg tctgcaaaac cattcacgac ccatcacaac
     2401 gctctggata tggaattgtt tcttcgcatc gcgcctgagc tctatctcaa gagacttctg
     2461 gtgggaggat tttcaaaagt atacgaaatc aatcgcaact ttcgaaacga aggaatcagt
     2521 tttaaacaca atccagaatt cactatgctc gaactctatg aggcctacgg ggatatgaat
     2581 tcgatgctgg atttgtgcga acgtctgttc gtcgatctcg cggagcgcgt gatcggttcg
     2641 actaaattgc ctttcgaaga agacttcatt gaactgaaaa gaccctggga aagactcacg
     2701 tactttgacg ccatcaagaa atacgcgaaa atcgatttga aagagaacat gactggcgag
     2761 caggcggtgg ctgcggcaaa ggcggcgggt ctgcgcgatg aagagctggc tcatgccgat
     2821 tcagtctgga cggttgcaga atgcatcttc gacgcaagag tggaagagaa gctcatgcag
     2881 cctgtgttta tcacgcatca tccaattgca gtttctcctc ttgcaaaggc agatcccgcg
     2941 aatcgaaatt tcgcactcag atttgaaacc tacattgccg gaagagagct gtgcaatgca
     3001 tttagcgagc taaatgatcc tatcgatcaa aaggcgcgct tccaggcaca ggtggagctg
     3061 cgcgaaaaag gatcggaagg cggcggttac atggatcatg attatattcg cgcgctggaa
     3121 tatggaatgc ctcctgcagg cggtatggga atcggaatcg ataggcttgt gatgctattc
     3181 accaacagcc acacaattcg cgatgtgatt ctgtttcctg ttctgcgtcc cgagaccgca
     3241 ggctctgact gattacagat attttacaac aaattcaatg agctttcgca ctgtatttgt
     3301 gtagggcgga aacatgagct tcatgggcgc aaagcgatga tgcttgagca cggcacgttc
     3361 gtgcgagaat gcgcgaaacc cgtagaagcc gtgcgaattg ccgctgcctg agaaattcac
     3421 cccgccaaag ggaaggttgg gatgaaggaa gtgaatgaga gtttcgttga tgcaggtccc
     3481 gccggaagtt gtctctgaca gcacatactt gatgtggcga ttgctcttgc tgaaaaaata
     3541 aagagccaga ggtttttcaa gcttcgagac gatcccaaga ccttcctcca gtgaatcaat
     3601 cgggatgact ggaagaatgg gtccaaagat ttcctcctgc atcacaggcg cgctcagcgg
     3661 cacatccgag agtagagtag gggctatgta ttgttccgtt gtgtctgtga ttccaccaaa
     3721 ctccagtttg gcgccttgct ctacgctttt ggcaaccata tcgctcaaac gcgcatggtg
     3781 ccgactgctg atgatgcgcg catagtgtgg actttgtttt ctttcttctt cgctttttcc
     3841 atagtaggaa gcaactgtct tcttcatccc ttctatgaat tccgcataac ggtctttgtg
     3901 aaccagggcg taatctggag caatgcacgt ttgcccgttg ttcataaatt tcccccagga
     3961 gatcttcttc accgcgtccg ccat
//
LOCUS       DAORFF010000365         3973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13734_length_3973_cov_3.791730, whole genome shotgun sequence.
ACCESSION   DAORFF010000365 DAORFF010000000
VERSION     DAORFF010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13734_length_3973_cov_3.791730"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PKK76_13625"
     CDS             complement(<1..599)
                     /locus_tag="PKK76_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69567.1"
                     /translation="MRIVPLVIALVLIKPLQADDFFLSACDYTLTLSGYVAQQGVDSL
                     KAITVREQPWTLFVVCTPNSRRTSDISEIQDRAKENGDFIRGYHEFDFSNGQRAAIFR
                     NTTRTNPVVRLEGYFATRDFEYRFVFVPEKGKTLRQEHWTAMEAELQSIISASRPGKQ
                     SPALPEASYRFRLSMFLAGLALAIGILSFFALRSILRRK"
     gene            652..867
                     /locus_tag="PKK76_13630"
     CDS             652..867
                     /locus_tag="PKK76_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69568.1"
                     /translation="MRIIQGILKRSSKRAYLAMYDANGILRIVRLTGMPPCQSGNTVT
                     LHGDFSSGVFQVKSARPHRFPRLLEAG"
     gene            complement(892..1815)
                     /locus_tag="PKK76_13635"
     CDS             complement(892..1815)
                     /locus_tag="PKK76_13635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNL69569.1"
                     /translation="MKYESIHAIVNPASSGGEAGRRWPETQKRMSSVLKNFSFELTSA
                     PGDGERLASAAIHEGVQSIVVIGGDGTLSEVVNGVLHFSESQRPRIGIINHGTGGDFA
                     RTIGAPTDIQLVLEKILSARDHAVDAGRITFTNHQGVQAKRYFVNVAGCGLAGEVVKA
                     INSSSRRFGAFSYFSTSFTNAITYKNAKVRISLDGGPETTETITTLAICNGQFFGGGM
                     QIAPDASITDGLLDVTILGNWSLAQKVRYSRNLYNGTISHCYGVKTARVKTIRIEPDN
                     LNPVLIDCDGEAVGKAPLTAEVMPGALRLIL"
     gene            1896..2924
                     /locus_tag="PKK76_13640"
     CDS             1896..2924
                     /locus_tag="PKK76_13640"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL69570.1"
                     /translation="MSRRNRTRLLSVIIASFVSALIGFVYSFTFNQSIFGGIVTGFTM
                     GFVVLSFEWFVLQGRYAHRTRNVSPPVFLLISLVCWTILLFTILRISHSLFAQPGDVI
                     PMWSWRTARGMILGVVMISGINLFLRTKYLVGMRVLGNFFLGRYNRPVQEDRIFMFLD
                     LEGSTAISERLGALRTHKLIRRFFQDVAEHVYEFGGETHRYVGDEVVVTWPTNQGLKN
                     AAWVNCIFDIRQAIDAERFSYYEEFGVMPEFRIGLHCGEVVAGEVGEDKREIVYIGDT
                     VNTAARLEQLCREKGTWLLVSEDILSRTELPSHIESKSLGPVTLRGRSKSMEVFTLRP
                     MKKLDSET"
     gene            complement(2945..3787)
                     /locus_tag="PKK76_13645"
     CDS             complement(2945..3787)
                     /locus_tag="PKK76_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNL69571.1"
                     /translation="MSLAELIAGIPENPRSRIAPTPSGLLHRGNAFNFLLTWTLVRQK
                     QGTLHLRIDDLDSARRRKEYIEDIFESLHWLGIDWDSGPRNSTEQQNIFSQDLRLRRY
                     HDLIQRLIDHGHCFACTCSRTETGPVYPGTCEAKNLPLDEPGTSIRLRVAQGEIVQIK
                     DRVTTSFAIGPETGSFVIRRKDGLPSYQIASVSDDEDMQMNLIVRGMDLLPSTAMQTL
                     IAEKAGLLNFKNASFIHHSLITKDGEKLSKSAGAESLRVLRQGGEEAAKSMRREFERW
                     ANAL"
BASE COUNT          912 a          969 c         1026 g         1066 t
ORIGIN      
        1 ttcttcctcc gaagaatgct tcgaagcgca aaaaagctta gaattccaat ggccagtgcg
       61 agccccgcca gaaacatact caggcgaaag cgataggaag cctccgggag tgcaggagac
      121 tgcttcccgg gtctggaagc agatatgatg ctttgaagct ccgcctccat ggctgtccag
      181 tgttcctgcc gtaaggtttt gcctttttcc ggtacaaaca cgaatcgata ctcgaagtcg
      241 cgtgtagcaa aataaccttc taaccttacg actggattgg tccgggtggt attgcgaaaa
      301 atggcggctc gttggccgtt tgaaaaatca aactcatgat agccgcgaat aaaatcgcca
      361 ttttcttttg cccgatcttg aatttcagaa atgtcgctgg tgcgccgtga attgggagtg
      421 catacaacaa acagagtcca gggttgttcg cggactgtga ttgccttgag tgaatccact
      481 ccctgttgcg caacatagcc agaaagggta agagtgtagt cgcacgcaga aagaaaaaaa
      541 tcatccgctt gaagcggctt gatgagcacc agtgcaatta caagcggaac gattcgcatg
      601 ttgatcacct tttgaagtca ggcccggccg ggtcaactgc aaggagcgaa gatgagaatc
      661 atccagggaa ttttgaagcg tagttcgaag agagcctatc tggccatgta tgatgcaaac
      721 ggcattctca ggattgttcg attgactgga atgccgccct gccagtcagg aaatacggtg
      781 actctgcatg gcgatttttc ttccggagtc ttccaggtaa agtcggcgcg gccgcacaga
      841 tttccgcggt tgctcgaagc aggctgaggg tttcgttgag tttggtttct ctcagagaat
      901 gaggcgaagc gcacctggca tcacttctgc agtaagtggc gctttgccca ccgcttctcc
      961 gtcgcaatct atcaggacag ggttcagatt gtctggttcg atgcggatgg tcttcactct
     1021 cgccgtcttg actccgtaac agtgagagat tgtgccgttg tacagattgc gagagtagcg
     1081 cactttctgt gcgaggctcc agtttccgag aatcgtcaca tcgagtagtc cgtctgtgat
     1141 gcttgcatcg ggcgcgattt gcattccgcc tccgaagaac tgaccattgc aaatcgcaag
     1201 agttgtaata gtctccgttg tctccggtcc accatccagg ctgattctta ctttcgcatt
     1261 cttgtatgtg atcgcgttgg tgaatgatgt tgaaaaatag ctgaacgctc cgaatcgacg
     1321 tgagcttgaa ttgattgctt tcacaacttc tccagccaga ccgcatcccg ccacattgac
     1381 aaagtatctc tttgcctgga caccctgatg attcgtgaac gtgattctac ctgcgtccac
     1441 tgcatgatcg cgcgcagaca aaatcttttc gagcacgagc tggatgtccg ttggcgcacc
     1501 gatggtgcga gcaaagtcac cgccagttcc gtgattgatg attccgattc tgggtcgctg
     1561 tgattcagag aaatgaagaa cgccgtttac cacttcggaa agagttccgt caccgccaat
     1621 gacgacgatg ctttgaacac cctcatgaat cgctgcagat gccaatcgct caccgtctcc
     1681 tggtgcgctg gtgagttcaa aactgaaatt cttcagcaca gaactcattc ttttctgggt
     1741 ttcgggccag cgcctcccgg cttcaccccc gctggatgct ggattcacga tggcatggat
     1801 ggattcgtat ttcatgaaag gggtagacaa tcgagatccc ggggaacaca aatgtaaact
     1861 acttttcggc gcgacagcca ctcgtggtca gaagaatgtc cagacgcaac cgcaccagac
     1921 tgctctctgt aataatcgcg agctttgtct ctgcgctcat cgggttcgtt tacagtttca
     1981 cattcaatca atccatattc ggcgggatcg tcaccgggtt caccatggga tttgtggttt
     2041 tgtctttcga atggtttgtt cttcagggta gatacgctca ccggacgcgc aatgtctccc
     2101 ctccagtgtt tctattgatt tcccttgttt gctggactat tctgctcttt accattcttc
     2161 gcatttcgca cagtctcttt gcgcagccag gtgatgtaat tccgatgtgg tcgtggagaa
     2221 ctgcacgagg aatgattctc ggcgtagtga tgattagcgg aatcaatttg tttcttcgca
     2281 caaagtatct cgtgggtatg cgcgttctcg gaaacttttt tctgggaaga tacaatcgtc
     2341 ccgtccagga agatcgcatc ttcatgtttc tggatctaga aggatctact gcgatttcag
     2401 aaagactcgg ggcccttcgc actcacaaac tcattcgccg tttctttcag gacgtggcag
     2461 agcatgtcta cgaattcgga ggagaaacac atagatacgt gggcgatgaa gtagttgtga
     2521 catggccaac gaatcagggt ttgaaaaacg ctgcctgggt gaattgcatc tttgatattc
     2581 gccaggcgat tgatgcagag cgtttctctt actatgaaga atttggagtc atgccagaat
     2641 ttcgcattgg acttcattgc ggagaagtgg ttgcgggaga agtaggagaa gacaagagag
     2701 agattgttta cataggagac acagtcaaca ctgcggccag gctagaacaa ctttgcagag
     2761 aaaagggaac ctggcttctt gtttcggaag acattctatc acgcactgag cttccttctc
     2821 acatcgagtc caagagcctg gggcccgtta cattgcgcgg aagatccaag tccatggaag
     2881 tattcactct gcgtcccatg aagaaattgg attctgaaac ctgagcgcga atctctgctt
     2941 tgcgtcaaag tgcattcgcc catctttcga attcacgacg catgctttta gccgcttcct
     3001 ctccgccctg cctgagcact cgaaggcttt ctgctccagc ggactttgaa agcttttcgc
     3061 cgtcttttgt aatgaggcta tggtgaatga aagaggcgtt cttgaagttc aagagccctg
     3121 ccttttctgc gatcagggtt tgcatggctg tagaaggcaa aagatccatt ccacgaacaa
     3181 ttagattcat ttgcatatct tcatcgtctg atacggaagc gatctggtaa gaaggaagtc
     3241 cgtcctttct tctgatcaca aagcttcctg tctccgggcc aatcgcaaaa ctggttgtta
     3301 cgcgatcctt gatctgaacg atttcacctt gtgccacgcg aaggcgaatg gatgtgcctg
     3361 gttcatccag gggcaggttc tttgcttcgc acgttcctgg atacaccggt ccagtttcgg
     3421 ttctactaca cgtgcaggcg aagcaatgcc catggtcgat caacctttga atcagatcat
     3481 gatagcggcg gagtctcaaa tcttgagaga agatgttttg ctgttccgta gaatttcggg
     3541 ggccggagtc ccagtcgatt cctagccagt gcaacgattc gaaaatgtct tcgatgtatt
     3601 ctttgcgcct tcgagcagag tcgagatcgt ctatgcgcag gtgcagtgtg ccttgttttt
     3661 gtcggaccag agtccatgtc agcaggaagt tgaaagcgtt tcctctatgg aggagtccgc
     3721 ttggggtggg agcgattctg cttcttggat tttcaggaat gccagcgatc agttccgcga
     3781 gactcacgac acagttccga tctgcggatc tctggtgcaa gaatcgtttt ttttgctttt
     3841 ctcagttctg gtggctgcca cgatcacgca tgttctttca agaaagcatc tttcgctcaa
     3901 agcgagtact cgtgaccggg ggaggatccg gcataggcct ttgcatcgca cgtgacttcc
     3961 tcgcgcacgg agc
//
LOCUS       DAORFF010000366         3969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13757_length_3969_cov_3.467041, whole genome shotgun sequence.
ACCESSION   DAORFF010000366 DAORFF010000000
VERSION     DAORFF010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13757_length_3969_cov_3.467041"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PKK76_13650"
     CDS             complement(<1..601)
                     /locus_tag="PKK76_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69572.1"
                     /translation="MIRIIATIFCALLAMPAFADKVRVNLGFWPGELQPEQENRLLKD
                     DLIAGASVGRLDLEWTNKETYTIYPLGVQYMMDGIGPGTLIIGGNYIRYSPAYKFNAL
                     TLGPSIGIINLQNFKNSDWEAEAGYQLALLDKKLLVTPKFGARWHFQEYDYDELTIGV
                     GSYAKSFDSPFRANAKGTYVGSGVQYYVTPELSILGEYIM"
     gene            666..1421
                     /locus_tag="PKK76_13655"
     CDS             666..1421
                     /locus_tag="PKK76_13655"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNL69573.1"
                     /translation="MRVYGVRAALCTMFLFLIPAGLISAPAQEWMGFLDKSLAVPEGL
                     MTGRLTVSGKTGRSQVWDFNLYKKHTDLLYLFSTKRRGLELKLLYKSGGEVCWMWDLL
                     RASLFRKRDLEKFEPVLGSGFSFMDLSGALYESQYEPVDLREASTMVLKIKPVLPSGY
                     TEILAQMDREKKRPLRIDFHGKDKILIKTMRFTYDLPLLDPRARKRISMAAPAVQDVI
                     DLETGRISRIEFFSLDRAVSPDDSLFIPEFLNR"
     gene            1421..2521
                     /locus_tag="PKK76_13660"
     CDS             1421..2521
                     /locus_tag="PKK76_13660"
                     /inference="COORDINATES: protein motif:HMM:NF016643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69574.1"
                     /translation="MMIEQTLRLLLDGVGRSDEYEFYLNRFRTYDAPCFSFLVPDLEA
                     AVQARSSILASLDFLRKLELYPGILFAGLSSSDPRTISFGSSIQEDLLLSIEMEGPGL
                     IESRIRREAELARREKKIPVFYLRMPLEKAVHACARVTNRFQFLRVQGPIQTVSGPAP
                     YIDPHQAHEFASIQESSFSRFASGVLLQDPSLHISLCSPYRLLQEIFTVKGAGTIFRP
                     RSMVHSYTKLESVDQERLIALLESSFGRRLRDLSSLHRASHFYIEENYRGAVLLEPAE
                     PGWYLSKFAVSTLARGEGIAQDLWTAVLDTHSSVFWRAKRGNPIERWYSRIADGFQRK
                     GMWNVYWKNLSLDRIADVIRYCENRPEDFDPS"
     gene            2582..3853
                     /locus_tag="PKK76_13665"
     CDS             2582..3853
                     /locus_tag="PKK76_13665"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNL69575.1"
                     /translation="MEKKQVVIVGGGFGGINAARELSDVKGVHVTLIDKRNHHLFQPL
                     LYQVAMAALSPAEIAAPIRSVVRHSGNVDVLLGTVTGVDLNARKVKADFGEVSYDYLI
                     MACGSTHSYFGHDEWEENAPGLKSLEEALEIRRRVLTAFELAEREHDPVVQEELLTFV
                     IVGAGPTGVELAGALSEISHYTMGHDFRNIDPRRTRVYLIEGGPRVLPPFDPRLSEYA
                     QKALEKLDVIVKTNSIVTKVHEHGVVIGNEEIRARTVLWAAGVQPAEINKTLGVATDR
                     GGRVIVERDCSLSAHPEVFVLGDQAAFEAGAGKYLPGVATVAIQQGRYVASLIRQELK
                     GKERKPFQYWDKGSMATIGRALAVAQTRRIRMKGFFAWIGWLLVHIYYLIGFENRMVV
                     LFQWAYNYLTMGRGARIITNRDWHSHTKKNS"
BASE COUNT          931 a          934 c         1065 g         1039 t
ORIGIN      
        1 tcatgatgta ctcacccaga atggaaagtt caggtgttac gtagtactga acaccggaac
       61 ccacataggt tcctttcgca tttgcgcgaa atgggctatc aaaacttttc gcgtacgaac
      121 ccaccccaat ggtaagctca tcgtaatcgt actcttgaaa gtgccagcga gctccgaact
      181 tcggagtcac aagaagcttt ttgtccagaa gcgcgagctg gtaacccgct tccgcttccc
      241 agtcagagtt tttgaaattc tgaaggttga tgattccaat gcttggtcca agcgtgagag
      301 cgttgaattt atacgcaggc gaatatcgga tgtagttgcc accgatgatc aatgttccag
      361 gtccaattcc gtccatcatg tactgaacgc ccagtggata tatggtgtag gtttctttgt
      421 tggtccattc tagatcgagg cggcctacgc ttgcgcctgc gatgaggtca tctttgagga
      481 gacgattctc ctgttctggc tgtagctctc ctggccagaa gccaaggttg acgcgcactt
      541 tgtctgcgaa ggcaggcatg gcgaggagcg cgcagaagat ggttgcgatg attcttatca
      601 tgccgccatg aaagacataa tcagacatag ggtcaacctt ttcgatcgac accggaacgg
      661 gtaccttgag agtttacgga gtgcgggccg ctctttgtac aatgtttttg tttctgattc
      721 ctgcggggct tatttccgct cctgctcagg aatggatggg cttcctcgac aaatccctgg
      781 cggttccgga aggtctgatg accgggcgac tgaccgtgtc gggtaagacc gggcgctctc
      841 aggtctggga cttcaatcta tataagaaac acacggatct actctatctg ttttccacaa
      901 agaggcgcgg tcttgagcta aagcttcttt ataaaagcgg cggagaagta tgctggatgt
      961 gggacttgtt gcgagcttct ctctttagaa aaagagatct ggagaaattt gaaccagtgc
     1021 tgggaagtgg attttctttt atggatttgt ctggagcact gtacgaatct cagtatgaac
     1081 cggtggacct gcgcgaagcg tcgactatgg ttctcaagat caaacctgtg cttccgtccg
     1141 ggtacactga aattctagct cagatggata gagagaaaaa gcgcccgcta cgaattgatt
     1201 ttcacggcaa agacaagatc ctcatcaaga ccatgagatt cacgtacgac ttgccgcttc
     1261 tggatccacg tgctcgaaaa agaatttcca tggccgctcc tgcagtgcag gacgtaatcg
     1321 atctggaaac aggcagaatt tctagaattg aatttttcag tctggaccgc gctgtttccc
     1381 cggacgattc acttttcata ccggagtttt tgaatcgctg atgatgatcg aacaaacgtt
     1441 acgattgctt ctcgatggtg tgggcagaag cgatgaatat gaattctatc tcaatcgctt
     1501 tcgcacgtat gacgcgccgt gcttttcttt tctggtgcct gatcttgagg cggcagttca
     1561 ggcgcgctct tctattcttg cgagtcttga ttttttaaga aagctcgaac tttatcctgg
     1621 catcctgttc gcgggtctgt cgagttctga ccctcgcact atttcttttg gatcctcgat
     1681 tcaggaggac cttcttcttt cgattgaaat ggaaggtccc ggactcatcg aatcaaggat
     1741 tcgcagagag gcagagcttg cacgccgcga aaagaagatc cctgttttct atttgcggat
     1801 gcccctggaa aaagcagtgc atgcctgtgc acgagtcacc aatcgctttc aattcctgag
     1861 agtgcagggc cccattcaga ccgtgtccgg tcctgctcca tacattgatc ctcatcaggc
     1921 gcacgagttc gcatcgattc aggaatcttc tttttcgcgt ttcgcttctg gcgttcttct
     1981 tcaggatcca tcgcttcaca tttcgctttg ttcaccttac aggctgttac aggaaatatt
     2041 tactgtgaag ggagccggaa ccattttcag gccacgttcg atggttcaca gctacacaaa
     2101 actggagagc gtggaccagg agcgtttgat tgcgcttctc gagtcaagct tcggtagacg
     2161 attgcgcgac ctgtcttctc ttcatcgcgc ttcgcatttt tacatagaag agaactatcg
     2221 cggtgcggtt ctgctggaac cagccgagcc gggctggtat ctttcgaaat ttgcagtcag
     2281 tactctcgcg cgtggagaag gaatcgcgca ggacctgtgg actgcagtgc tcgacacgca
     2341 ttcaagcgta ttctggcgag cgaagagagg caatccaatc gagagatggt acagccgtat
     2401 tgcggatggt tttcagcgca aagggatgtg gaatgtttac tggaaaaacc tgagcctgga
     2461 tcgcattgct gacgttattc gatattgcga aaatcgtcct gaggattttg atccgtcctg
     2521 atttttctac tttgactcgc agggcccctt tgtttttttg aatctgccag ggagcgaacg
     2581 catggaaaag aaacaggttg taatcgtagg cggaggattc ggtgggatca acgctgcgcg
     2641 tgaactgtcg gacgttaagg gagttcacgt aactctgatc gacaagcgaa accatcatct
     2701 cttccaaccg cttctttatc aagtcgccat ggccgctctg agcccggctg agattgcagc
     2761 tcccattcgt tctgttgtgc gccattcggg gaatgtggat gtgcttcttg gcacggtcac
     2821 aggtgtggat ttgaatgcgc ggaaggtaaa agcggatttt ggggaagttt cgtatgatta
     2881 tctgatcatg gcatgcggat cgactcatag ctatttcggt cacgacgagt gggaagaaaa
     2941 tgccccgggc ttaaagtccc tggaagaagc gcttgagatt cgaagaagag tgctcactgc
     3001 ttttgaactc gcagaacgcg aacacgatcc ggtggttcaa gaagagctgc tcactttcgt
     3061 gatcgtaggt gctggtccaa ctggcgtgga actggccggc gctctatctg aaatcagtca
     3121 ctacaccatg ggtcatgact ttcgcaacat tgacccgcga agaaccaggg tgtatctcat
     3181 cgaaggtggt cctcgagtac tgcctccctt cgatcccagg ctttctgaat atgcacaaaa
     3241 ggcgcttgag aagctcgatg tgatcgtaaa gacaaacagc atcgtaacaa aggttcatga
     3301 gcacggagta gtcattggaa acgaggagat tcgtgctcga acggttctct gggcggccgg
     3361 tgtacagccc gctgaaatca ataagaccct gggtgtggca acggacagag gcggtcgcgt
     3421 cattgtagag agagattgca gtctatccgc acatcccgaa gtattcgtcc tcggagatca
     3481 ggcagcgttt gaagccgggg cgggtaagta tctgccaggt gtggccacgg ttgcgattca
     3541 gcaagggcgc tatgttgcca gcttgatcag acaagagcta aaaggtaaag aacgtaaacc
     3601 atttcaatac tgggataaag gcagcatggc tacgatcggt cgtgctcttg cggtagcaca
     3661 aaccagacgc attcgcatga agggattctt tgcatggatt ggctggcttc tcgtgcacat
     3721 ttactatctc attggatttg aaaacaggat ggtggttctg tttcagtggg cgtacaacta
     3781 tttgaccatg ggtcgagggg cgcgcatcat cacgaatcga gactggcaca gtcatacaaa
     3841 gaagaactcg tgatcagacc cataccggaa tctgaatgaa agcctgggta aagctcagtc
     3901 tcacagcttt cttctgggca ggggctttct tcgcaggaaa ggcggcggtc acaatcgcgc
     3961 ctcctgtga
//
LOCUS       DAORFF010000367         3968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13763_length_3968_cov_3.417071, whole genome shotgun sequence.
ACCESSION   DAORFF010000367 DAORFF010000000
VERSION     DAORFF010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13763_length_3968_cov_3.417071"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="PKK76_13670"
     CDS             <1..1257
                     /locus_tag="PKK76_13670"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL69576.1"
                     /translation="KLYDDLRNRADELNCLYEISQKIGFQNKREDLLDTIITAIGEVL
                     VVERVSVLLREGPDSTLKVARLRGFSIDDHDVRIDPEEGIAGIVFKTGDPLLVRDMEK
                     DLRIMADKSSRYKTRSFIAVPIFRDGQVAGIINAADKKNGEAFDLFELQVLSTVATQL
                     ADGLSRIDAKQREVQLQQYRKDLETAAQIQINSLPEIPSRIAGLQIAYRYQACRDVGG
                     DFYDLIFHSENRISVLIADVAGKGVPAALFMEYSKTLLASHIPRNLDPVTTLTRVNQE
                     IFRKSRTGIFVTSMLVQIEIDLKRLRVASAGHNNQLLLRTRRGKVEKISARGTPLGIF
                     ENTEYIENVVSFEKDDILLLYTDGVTEANNPALEEYGDERLMSIVESARGLEPHAIVE
                     KLFQDLAVFCGSAEQADDVTVVAVRL"
     gene            1360..2307
                     /locus_tag="PKK76_13675"
     CDS             1360..2307
                     /locus_tag="PKK76_13675"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNL69577.1"
                     /translation="MGDLLKLLKSAADETRLRILSVVESEPLSVGEILEILDMGQSRI
                     SRHLKILADAGFLESNRAGNRVYYGLSAASRQEPFVHELLANLSTTAEDATYRGNGLA
                     GGLIQDRRKLTGILELRKKETREHFQKYGRDHDRLQRAYVDADFYRKEIARELGKGAG
                     VVVDLGCGTGELSALISAQARRIIGVDQSTKVLDLARKECPSGDFRLGELEHLPLKNE
                     EADAVVASMVLHHLPDPLTGLREANRVLRKNGALVVADLKQHEDEEAQKELADFWPGF
                     EKSRLETMIRDSGFEITEHRSGKGSGRLTCLIYRAKKTK"
     gene            2318..3616
                     /gene="ahcY"
                     /locus_tag="PKK76_13680"
     CDS             2318..3616
                     /gene="ahcY"
                     /locus_tag="PKK76_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017855632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNL69578.1"
                     /translation="MQTLKKNDYKVKDISLARWGREEIILAEKEMPGLMALRKEYGKD
                     KPLRGAKIAGSLHMTIQTAVLIETLAELGADIRWSSCNIYSTQDHAAAAIAEAGIPVF
                     AWKGETEEEYWWCVEETLKFDGGPNMILDDGGDLTWWVHEKHPEMLKNIRGVSEETTT
                     GVHKLVKEFELGNLMIPAINVNDSVTKSKFDNLYGCRESLADGIKRATDIMLAGKVAV
                     VAGYGDVGKGSAHSLKAFGSRVVVTEIDPICALQAAMEGYHVDLMDEWADKADIFVTT
                     TGVKDIITIKHMEKMRDGAIVCNIGHFDVEIQVAELKAYPGIKKTEIKPQVDKYTFPS
                     GKSIILLADGRLVNLGCATGHPSFVMSNSFTNQVLAQIELWTNKYEIGVYRLPKHLDE
                     KVAALHLEKIGVKLTKLSEDQAKYLGVPVAGPYKPEHYRY"
     gene            3698..>3968
                     /locus_tag="PKK76_13685"
     CDS             3698..>3968
                     /locus_tag="PKK76_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016757870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNL69579.1"
                     /translation="MNRISLQQLREILAKRILVLDGAMGTMVQRYNLTDSDFRGERFK
                     NHPKELKGNNDILVLTRPDVISAIHKEYLAAGCDIIETSTFSCTTI"
BASE COUNT         1082 a          898 c         1070 g          918 t
ORIGIN      
        1 aagttgtacg atgatctgcg aaaccgcgcg gacgagttga actgtcttta tgagatttcg
       61 cagaagatag gatttcagaa taaaagagaa gatttgctcg atacgatcat cactgcaatc
      121 ggggaagtgc tcgtagtaga aagggtgagt gtgcttttgc gggaggggcc ggatagcact
      181 ctgaaagtgg cacgccttcg cggtttttct attgatgatc atgacgttcg cattgatcca
      241 gaggaaggaa ttgcgggaat tgtttttaag accggagatc cgcttctggt tcgcgacatg
      301 gaaaaagatc tgcgtattat ggcagacaag tcgtccaggt acaagaccag gtcttttatt
      361 gcggtcccca tctttcgcga cgggcaggtg gctggcatca tcaatgctgc ggacaagaaa
      421 aatggagagg ccttcgatct atttgaactc caggttcttt ccacagtcgc aacacagctt
      481 gcagacggtt tgtctcgcat tgacgcaaag caaagagaag tgcagcttca gcaatacaga
      541 aaagacctgg aaacggcagc gcagatccag atcaattctt tgccggagat tccttcgcgc
      601 attgcaggtc tgcagatcgc ctatcgttac caggcctgcc gcgacgtggg cggcgacttt
      661 tacgatttga tctttcattc tgaaaataga atttcagtac tgattgcgga cgtagcaggg
      721 aagggagttc cagcggctct ttttatggag tactccaaaa ctctccttgc gtcccatatc
      781 ccgcgaaatc tggatcctgt gaccactctg acccgggtga atcaggagat ctttcgcaag
      841 tcccgcactg gcatttttgt tacatccatg ctggttcaaa tcgaaatcga tctgaaacgc
      901 ctgagagttg cctccgccgg ccataacaac cagcttcttc ttcgcacccg ccgggggaag
      961 gtggagaaga tatctgcgag aggaacacct ctcgggatct tcgagaatac agagtacatc
     1021 gaaaacgtgg tcagttttga aaaagacgat attcttctat tatatacgga cggagtcaca
     1081 gaagctaaca atccggccct cgaagaatac ggcgatgaaa ggctcatgag cattgtggag
     1141 tctgccagag gccttgaacc gcatgcaatt gtggagaagc tgtttcagga tctggctgtt
     1201 ttttgcggtt ctgcagaaca ggcagatgac gtcactgtcg ttgcagttcg cctttgaggc
     1261 cgcccagagc tagaattcac ccattcagtc gccgattgcc ttgaattcgg cacagtaaaa
     1321 gggtggactc tatatcagga tatcttgata ttggtataga tgggagatct gctaaagttg
     1381 ctcaagtccg ccgcagatga aaccaggctg cgtatcctca gcgtcgttga atccgagcct
     1441 ctttctgtcg gggagattct cgaaatcctg gatatgggtc agagccgcat ctcgcgccat
     1501 cttaagattc tcgcagacgc aggatttctg gagagcaatc gggcagggaa cagagtgtac
     1561 tacggcctga gtgctgcgtc cagacaggaa ccgttcgtgc atgagcttct tgcaaacctt
     1621 tctacaacgg cagaagatgc aacgtatcga ggcaatggac tggcaggcgg tctcatccag
     1681 gacaggcgaa agctcaccgg aattctggag cttcgcaaga aagaaacaag agaacatttt
     1741 caaaagtacg gacgcgatca tgatcgtctg cagcgcgcat atgtagacgc agacttttac
     1801 cgcaaagaaa tcgcacgaga gcttgggaag ggtgcgggag tagttgtgga ccttggatgc
     1861 ggcaccggag aactttccgc gctcatttca gcccaggcgc gtcgaattat cggcgtagat
     1921 caaagcacaa aagtcctgga tctggcgcgc aaggaatgtc cgtcaggcga ttttcgtctg
     1981 ggcgagctgg aacatcttcc tttgaagaac gaagaggcgg acgcggtagt tgcatccatg
     2041 gtgttgcatc atcttcctga tcctttgacg gggcttcgcg aagcgaatcg agtactgaga
     2101 aagaacggag cacttgtggt tgcggatctc aaacagcacg aagacgaaga ggcgcagaaa
     2161 gaacttgctg atttctggcc gggctttgaa aagtccaggc tcgaaaccat gatacgcgat
     2221 tcgggatttg aaatcacaga acatagatcc ggcaaagggt ccggtcgact tacatgtctt
     2281 atttatcgag cgaaaaaaac caaataggag ggtttgaatg caaacgctga aaaagaacga
     2341 ttacaaagtg aaggatattt cactggcccg ctggggacga gaagagatca tcctcgctga
     2401 aaaggaaatg ccaggcctga tggctctgcg caaagaatac ggaaaagata agccgctgcg
     2461 cggagcgaaa attgcaggtt ctctgcacat gacgatccag actgcggttt tgattgagac
     2521 tctcgccgag cttggagcgg acattcgctg gtccagctgc aacatttaca gcacgcaaga
     2581 ccatgcagcc gcggccattg cagaagcagg aattcctgtg tttgcctgga aaggggaaac
     2641 agaagaagaa tactggtggt gtgtagaaga aactctgaag tttgacggcg gccctaacat
     2701 gattctggac gacggcgggg atctcacctg gtgggttcac gaaaaacatc cagaaatgtt
     2761 gaagaacatc cgcggtgtat ctgaagaaac aactacaggc gtacacaagc ttgtaaaaga
     2821 attcgaactg gggaatctca tgattcccgc gatcaacgta aacgattctg taaccaagag
     2881 caagttcgac aacctgtatg gttgccgcga atctctcgct gatggaatca agcgcgcaac
     2941 tgacatcatg cttgcgggca aggtggctgt ggtcgcagga tacggagatg tgggcaaagg
     3001 ttccgcgcac agtctcaagg catttggttc acgcgtcgtg gttactgaaa tcgatccaat
     3061 ctgcgcgctg caggctgcga tggaaggata ccatgtggat ctgatggatg agtgggcaga
     3121 caaagcggat atcttcgtaa ccactacagg tgtgaaagat atcatcacca tcaagcacat
     3181 ggaaaagatg agagacggtg cgattgtatg taacattggc cactttgatg tggaaatcca
     3241 ggttgctgaa ctcaaagcgt atccaggaat taagaagact gaaatcaagc cacaagtgga
     3301 caagtacact ttcccgagtg gcaagagcat cattcttctt gcagacggac gtcttgtgaa
     3361 tcttggatgc gcgacaggtc acccaagttt cgtaatgtct aacagtttca caaaccaggt
     3421 tctggcgcaa attgaactgt ggacaaacaa atacgaaatc ggcgtgtatc gtcttccaaa
     3481 gcatctggat gaaaaagtgg cagcgcttca tctcgaaaaa atcggagtga agctcacaaa
     3541 gctctcagaa gaccaggcaa aatatcttgg tgttcctgta gccggccctt acaagccaga
     3601 gcactaccgt tattgagaat tgctgtaaag caaacgagcg ggtgcgccga attttcggcg
     3661 cgcctgcgca tctttttgga atgagttatt atacattatg aacagaatca gccttcaaca
     3721 actccgcgaa atcctggcaa agcgcatcct cgttttagac ggtgcgatgg gcacaatggt
     3781 gcaaaggtac aatcttacgg attctgattt ccgcggtgag cgcttcaaga atcatcctaa
     3841 agagttgaaa gggaacaatg acattctggt tctcacccgg ccagatgtaa tctctgcgat
     3901 ccataaggag tatctggctg caggctgcga tattatagaa acatctactt ttagctgtac
     3961 gaccattg
//
LOCUS       DAORFF010000368         3964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13781_length_3964_cov_4.046048, whole genome shotgun sequence.
ACCESSION   DAORFF010000368 DAORFF010000000
VERSION     DAORFF010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13781_length_3964_cov_4.046048"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PKK76_13690"
     CDS             complement(<1..946)
                     /locus_tag="PKK76_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNL69580.1"
                     /translation="MVSFGLAVDDSGESRIDLGGYQLDGFYDEMFRPDGTLRPAYEGF
                     SSAISGWSREELLRRKRMAERSLLNRGITFQVYGDASRKEQIFPFDAIPRIIEAKEWK
                     QMEKGLKQRTFALNEFINDIYHDKKIVKDGVIPTWLLASAKSYLPVCEGLNPPRGIWC
                     HINGVDLVRSGDGRYYVLEDNLRCPSGVSYVLVNRENMKRTFPEIFLKQGVLPVYDYP
                     YKLKKMLSYISGKEDPVLGVLTPGQYNSAYFEHGFLAQQMGAELVEGSDLIVYQHEVY
                     MKTTRGLKRVDAVYRRIDDTFMDPLAFRPDSMLGVPGIF"
     gene            complement(1064..1948)
                     /locus_tag="PKK76_13695"
     CDS             complement(1064..1948)
                     /locus_tag="PKK76_13695"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNL69581.1"
                     /translation="MVRYRIVHKTTYQYSEHVSLCHNEAQLSPRMTDFQIPRDTRLFV
                     SPQPAVLSERTDYFGNRLFYFAVQSPHNVLEVTSESVVDLLPRTGPAGSNPILPPSPA
                     WETVRDDVRSSWDLFQYLNESTHVRATDAVRQFALVSFFPGRPILEAAWDLNVRIHSE
                     FRYEPGSTRITTTTDEVLRIRKGVCQDFSHLAIACFRSLGLPARYVSGYIESAPQRGQ
                     KMQGADASHAWFSVFVSGAGWFDFDPTNRKMPDDQHITVSLGRDYADIAPLKGIVYGG
                     GKSVCKVAVDVMRQESPA"
     gene            complement(1930..>3964)
                     /locus_tag="PKK76_13700"
     CDS             complement(1930..>3964)
                     /locus_tag="PKK76_13700"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNL69582.1"
                     /translation="RRYLHFYAADLARSSNGCFTVISDRLQSPSGAGYALENRIALSR
                     TLPSLYRDSHVHRLALFFRTLRNHLSQLAGGGSRLVILTPGPENETYFEQAFLANYLG
                     FNLVQGSDLTVRDGKAYLKTLNGLRPIDGIFRRIDDAYLDPLELKNDSLLGVAGLMYC
                     IRSGSVAVMNPPGAAVLENPGIYPFLPRICKFLTGQELRLAGAPTYWCGDSLSSVLSR
                     LSDLVIKPIARGWERGTIYCSRLSSAELEDVRRRIQANPAGFIAQEKLPLSTTPLWTG
                     EKFEPRPMVIRSFACARDESFMIMPGGLVRVSPDPDSTVVSNQKGGVSKDLWILASEP
                     EKQISLLSTSMSTPIVRSGGEIPSRIADNLFWIGRYAERTEALARILREVYLRLVEMQ
                     GDEEVFDILLLALEPHCGLLDPELVRKHSGSPEVERKLRAIVFDASILGGLKFDVRAL
                     NTCGRALRERLSEDAWRTLNRLEGELDRPDKSILETLESTLLYLASFAGFVSESMTRG
                     QGFRFLELGRRIERAGLAADLLMHATRSKAPDALWEHVLRIQNSYMTYRRRYRTRMDP
                     IAVVDLLVFDESNPRSVAHQLIRLEDLTRGLPEWKEAGKSAESRIALHALSLMRLADG
                     AVRSADDDYRELQSLLSQEKNYLRTLAETISLHYFSYIETQQTLEANQWSVTG"
BASE COUNT         1042 a         1045 c          980 g          897 t
ORIGIN      
        1 caaaaattcc aggaacaccc agcattgaat ccgggcgaaa cgcaagagga tccatgaacg
       61 tgtcatcaat cctacgatag acggcatcga ctcgcttgag cccgcgagta gtcttcatgt
      121 atacttcgtg ttgatataca atcaggtcag aaccttctac cagttccgct cccatctgct
      181 gagccagaaa gccatgttcg aaatacgcag agttgtactg accaggagtg agaacaccca
      241 gaaccgggtc ctcctttcct gagatgtagg aaagcatctt cttgagtttg tacggatagt
      301 cataaacggg aagcacacct tgcttgagaa aaatctccgg gaaggtgcgc ttcatattct
      361 cccggtttac aagcacatag gaaacgccgg aaggacaacg caggttgtct tcgagaacat
      421 agtagcggcc gtccccggag cgaaccaggt ccactccatt gatgtgacac cagattcctc
      481 ttggaggatt caatccttcg cataccggaa gatagctttt cgcggaggca agtagccagg
      541 tcggaatgac accatccttc acaatcttct tgtcgtgata gatatcgttg ataaactcat
      601 tgagcgcgaa ggtgcgctgc ttgagtcctt tctccatctg cttccattct tttgcttcga
      661 tgatgcgcgg gattgcatca aaaggaaaga tctgctcttt gcgcgaagcg tcgccgtaga
      721 cttgaaacgt gattccgcga ttcagcaggg atcgttctgc cattcgcttt cgcctgagca
      781 actcttcccg actccagccc gagatcgctg agctgaaccc ttcataagca gggcgaagtg
      841 tcccgtccgg tcggaacatt tcgtcataga aaccgtcgag ctggtatccc ccgaggtcaa
      901 tgcgactttc gccgctatca tctactgcaa gcccaaatga aaccatacag ccataattgc
      961 aatgtccgta ccatggctgt acgcctcaat agaatgcaac gaaatggacc tgacgatcca
     1021 gcgtcgggga gagcttattc gaagggagca caatgctgaa ttttcaggca gggctttctt
     1081 gtcgcatcac gtccaccgcc accttgcaaa cacttttacc gccaccgtac acaattcctt
     1141 tgagcggcgc aatgtctgcg taatcacgcc ccagagacac tgtgatgtgc tggtcgtctg
     1201 gcatcttgcg attggtagga tcaaaatcaa accagccggc accagaaaca aaaacggaaa
     1261 accaggcgtg cgacgcatcc gcaccttgca tcttctggcc cctttgcggt gctgattcaa
     1321 tatagccgct gacgtatctg gcgggcaaac caagagatcg aaaacaggca atggccagat
     1381 gcgaaaaatc ctgacagaca cccttcctga tcctcaacac ttcatccgta gtcgtcgtga
     1441 tgcgagtgga accgggttca tatctgaatt cggaatgaat tcgcacgttc aaatcccacg
     1501 cagcctccag aatgggtctg cctggaaaaa atgagaccag agcaaactgg cgaactgcgt
     1561 ccgttgcccg aacgtgagta gactcgttca agtattgaaa gagatcccag gaagatcgaa
     1621 catcgtcgcg aacagtttcc caggcggggg aaggtggcaa aatcggattg gaacctgccg
     1681 gtccggtgcg aggcaaaaga tccaccacac tttctgaagt tacttccaga acgttatgcg
     1741 gcgattgaac tgcaaaatag aatagcctgt tgccgaaata gtccgtcctt tcgctcaaaa
     1801 ctgcaggctg cggagaaaca aaaagacgtg tatctctggg gatttgaaaa tccgtcatgc
     1861 gcggactgag ctgcgcttca ttgtggcata aagaaacatg ctcagaatac tgatatgtgg
     1921 ttttatgaac tatcctgtaa cggaccactg gttggcctcc agagtctgct gtgtttcgat
     1981 gtaactgaaa tagtgcagac tgattgtttc tgcgagcgtc cgaagataat tcttctcctg
     2041 cgaaagcaaa gactgtagtt cacgataatc gtcatcggcg gatcgaaccg ctccatccgc
     2101 gaggcgcatc aaggaaagcg cgtgcaaggc gattctactt tctgcactct tgcccgcctc
     2161 tttccattcc ggaagcccgc gagttagatc ttccaggcga atcaattgat gagcgacgga
     2221 gcgtggattg gattcatcga aaacaagtag atcaacaaca gcgataggat ccattcgtgt
     2281 gcgatatctg cgcctgtagg tcatgtacga attctgaatg cgaaggacat gttcccagag
     2341 tgcgtcgggc gctttggatc tggtcgcatg catcagcaga tctgcggcaa ggccggctct
     2401 ctcaattctt ctacccagtt ccaggaaccg aaacccctgg ccgcgcgtca tactttcact
     2461 cacgaaacct gcaaagcttg ccaggtacag aagagtgctt tcgagagttt ccagaatgct
     2521 tttatcagga cgatccagtt caccttcgag gcgattcaaa gttctccatg catcctctga
     2581 cagtctttcg cgcagagccc ttccgcatgt gttgagcgcg cgaacatcaa acttgagccc
     2641 accaaggatg gacgcatcga atacgatcgc cctgagcttg cgttctactt ccggcgaacc
     2701 ggaatgcttc cgcacgagct caggatccag aagaccgcaa tgtggttcca gggccagaag
     2761 cagaatatcg aatacttcct catctccctg catttccaca agacgaaggt acacttccct
     2821 cagtatgcgc gccaacgcct cagtcctttc cgcataacgt ccaatccaga acagattgtc
     2881 tgcgatgcgc gaaggaattt ctccaccgga acgtacaatc ggcgtggaca tcgaagtaga
     2941 caggaggctg atttgcttct ctggctcaga tgcaagaatc cagagatcct tgctcactcc
     3001 acctttttga ttggaaacaa cagtagaatc cggatccgga gatactcgaa ccagcccgcc
     3061 cggcatgatc ataaatgatt catcacgcgc acaggcgaag ctgcgaatca ccataggacg
     3121 aggttcaaat ttttcgcctg tccagagtgg tgtagtagag agagggagtt tttcctgagc
     3181 aatgaagcct gctggatttg cctgaatccg acgcctgaca tcttccagtt ctgcggaaga
     3241 caatctggaa caatagatcg tccctctttc ccagccccgc gcaattggct tgattactaa
     3301 atcggacaaa cgagagagaa cagaagaaag agaatcgccg caccagtagg tgggcgctcc
     3361 ggccagacgc agttcctgcc ccgtcagaaa tttgcagatt cgcggaagga atggatagat
     3421 tccaggattc tcaagaactg cagcacccgg aggattcatt acggccactg atccagatct
     3481 tatgcaatac ataagaccgg ccacgccaag aagagaatcg tttttcaact caagaggatc
     3541 caggtaagca tcgtcaattc tgcgaaagat tccgtcaatg ggcctgagtc cattcaatgt
     3601 tttgagatac gcctttccat cacggaccgt cagatccgag ccttgcacta gattgaatcc
     3661 caggtaattg gcaaggaatg cctgctcaaa ataggtttcg ttttccggac cgggagtaag
     3721 aatcaccaga cgacttccgc cgcctgcaag ctggctcaaa tgatttctga gagtgcgaaa
     3781 gaacaacgca agcctgtgaa catgcgaatc tctgtagaga ctgggtagag tgcgagaaag
     3841 tgcgattcgg ttttcaagtg cgtaacctgc accggaaggg gactgcaatc tgtccgaaat
     3901 cacagtgaag caaccattcg aggatcgcgc caggtccgcg gcataaaagt gcaagtaacg
     3961 gcgg
//
LOCUS       DAORFF010000369         3961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13790_length_3961_cov_3.621096, whole genome shotgun sequence.
ACCESSION   DAORFF010000369 DAORFF010000000
VERSION     DAORFF010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13790_length_3961_cov_3.621096"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PKK76_13705"
     CDS             complement(<1..307)
                     /locus_tag="PKK76_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69583.1"
                     /translation="MRSLASRTIVVVFTMGACVLLMLPNFLTQKLAVVFQENHRLEDG
                     KKVPVDLAEVRDFMRDRENGLGLYFPSAQCVPEAGDGIPAERRCVLEARFITAAAVNE
                     "
     gene            complement(315..689)
                     /locus_tag="PKK76_13710"
     CDS             complement(315..689)
                     /locus_tag="PKK76_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69584.1"
                     /translation="MRWLPLLLITAGLYAQEPQETPKPQEEPRKQEAPRKPPVAAPLP
                     KVQGLSDDFPIGDEKYLPEYKGETIAPPKETAQPEKKPDDSPKGPGFLAGLLSGDKTF
                     LNVLILCLLVGIFVLYRLRGRK"
     gene            complement(691..1062)
                     /gene="yajC"
                     /locus_tag="PKK76_13715"
     CDS             complement(691..1062)
                     /gene="yajC"
                     /locus_tag="PKK76_13715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNL69585.1"
                     /translation="MEFLRFLAQQPAAPPTTEAPAQAPGAAAAGPGSSLTMFLLPVAI
                     FAFMWLFVIRPQRKEEKKKREMVNTLKKGDEVVTTGGMIGTIATIKDDTVILNVGDKT
                     RLEFMKSHISQMRETKKAEAK"
     gene            complement(1113..2150)
                     /gene="trpD"
                     /locus_tag="PKK76_13720"
     CDS             complement(1113..2150)
                     /gene="trpD"
                     /locus_tag="PKK76_13720"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNL69586.1"
                     /translation="MQEYLKKVVSRQDLTAEEASHAMTAIMTGEAGEISTAALLTALA
                     MKGETPVEIEAFARSMRKAAVAWTGRSEDAILDTCGTGGDRSNTINLSTLSALVLASA
                     GLKVAKHGNRAVSSSTGSADLLEALGISLGVQSPEVLDQCLNQTKIAFLFAQAWHPAM
                     KHAGPVRRALGFRTVFNLLGPLTNPAPVTHQLVGVFDARFLEPVAATLLHLGRKAAYV
                     VHAENGLDEISPAGGTRYFRVQDGKVESGTWKPEDFGFSSFPLSAIVAKDKAASVDRA
                     RAIVSGQGTQEENAAISMNAAPLYAAVRGVDLKTAAGACEQLLKQGAPGKVLEAWQKF
                     TSEKDLSVGAG"
     gene            complement(2150..2839)
                     /gene="pgsA"
                     /locus_tag="PKK76_13725"
     CDS             complement(2150..2839)
                     /gene="pgsA"
                     /locus_tag="PKK76_13725"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNL69587.1"
                     /translation="MNLPNFLTVLRVISVPFFIYFLVQPSFTWRLTAFILFALASLTD
                     LVDGYLARKWNQQTEFGKFLDPLADKFLVLGAFITFVVLTEQVQVWMVLCIVGRDMLI
                     TALRYLGIRKGKSLRTSRFGKWKTALQMFSIVIILMSFLLVSYGDKKEINRIYAEGRS
                     QGIGPFDVAWFNLQEAMAGNHADWFYSLASFLPYYLMLITTIITILSGLRYLVTNYKL
                     LLPPYRVKEKV"
     gene            complement(2836..>3961)
                     /locus_tag="PKK76_13730"
     CDS             complement(2836..>3961)
                     /locus_tag="PKK76_13730"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNL69588.1"
                     /translation="RTVLDAGKVKKKHKKQKLVLVGCFTERYADTVRAELPEIDFSFG
                     TGIYHKAGELIRNAFGLSVGLEPVSPFFEMQAQGKPYAPVKISDGCDRGCSFCAIPLF
                     RGKFRDIPADEIIAECERLAAQGVREVFLVSQDSNSYGGKPEAYLDLVERIHEVQGLH
                     WIRMLYMYPDGKTERILEGMRTRSMPKVVPYLESPVQHASESVLRAMRRIGSSERFAD
                     LFALARDVMPGCEVRTSILLGFPGETDADVEKVEQFIATAKPEKLALFSYSPEEGTPG
                     AEMSAQIAPEIIADRIRRVQSVHRGVQSEHLRSLVGTTFTCMVDESSAEEVVLRRPQD
                     APEADGVVYAEPKSRLLHSGDLVNVEITGFTEYDLTGVIT"
BASE COUNT          967 a         1067 c          969 g          958 t
ORIGIN      
        1 tttcgttcac tgcggccgcc gtaatgaaac gcgcttcgag aacgcatctt ctttctgcag
       61 ggattccgtc cccggcctct ggaacgcact gagcggaagg aaaatacaga cccaggccat
      121 tttccctgtc gcgcatgaag tcgcgcactt ccgccagatc cacaggcact ttctttccat
      181 cttccagcct gtggttttcc tgaaacacta cagccagttt ctgagtgaga aagttgggca
      241 acatgaggag cacgcaggct cccatagtaa atacaactac aatggttctt gaggcaagtg
      301 atcgcataac ggtcctattt acgtccccga agtctataga gaacgaagat acctacgagg
      361 aggcagagaa tgagaacatt gaggaaggtc ttgtctccgg agagaagacc ggccagaaat
      421 ccaggacctt taggagagtc gtccggtttc ttttcaggct gcgccgtttc tttagggggt
      481 gctatggttt cccctttgta ttccggcaga tacttttcat caccaatggg gaaatcatca
      541 gacagcccct ggacctttgg aagaggagcg gcgaccggcg gtttgcgcgg cgcttcttgt
      601 ttgcgcggct cttcctgcgg ctttggtgtt tcctgcggct cctgagcgta gagcccggcg
      661 gtgatcagaa gaagcggaag ccagcgcata tcactttgct tcagctttct ttgtttcgcg
      721 catctggctg atgtgcgact tcatgaattc aagacgagtc ttgtctccaa cgttgagaat
      781 cactgtatcg tctttgattg ttgcaatggt gccgatcatg ccgcctgtgg tgactacttc
      841 atcgcctttc ttcagagtgt tcaccatttc gcgctttttc ttttcttctt tgcgctgagg
      901 acgaatcacg aagagccaca tgaatgcgaa aatcgcaact ggcaaaagga acatggtcag
      961 agatgaacct ggacctgctg ctgccgcgcc gggtgcctgt gcaggcgctt ctgtggtagg
     1021 aggggcggct ggttgttgcg cgagaaaacg taagaattcc atgcaaccag acgcccgatt
     1081 ctcgcctggc ttgcaactat aaacttctga agttagcccg cgcctacaga aaggtctttt
     1141 tcagacgtaa atttttgcca ggcttccagc accttgcctg gagctccttg cttgagcaac
     1201 tgctcacagg ccccggccgc cgttttaaga tctacacccc gaaccgcggc atagagagga
     1261 gccgcattca tagagatggc tgcgttttct tcctgagtgc cctgacctga aacgatcgct
     1321 ctggcgcgat ccacggatgc cgctttgtct tttgcaacaa tggcagaaag tggaaaagag
     1381 ctgaatccaa aatcctctgg cttccacgtt ccactttcca ctttcccatc ctgaactcga
     1441 aagtagcgag tgccgcccgc gggcgaaatt tcatctaaac cgttttctgc gtgaactacg
     1501 taagctgcct ttcttccaag atgaagaaga gtcgccgcca caggttcgag gaatcgcgca
     1561 tcaaacactc ccaccagttg atgggtgact ggcgcgggat tcgtcagagg gccaagcaag
     1621 ttaaagactg tgcgaaagcc aagagcacgc cggacagggc cggcatgctt catggctgga
     1681 tgccaggcct gagcgaacaa gaacgcaatt ttagtctgat tcaggcattg atcgagcact
     1741 tcaggactct gcacacccag agaaatgccc agcgcttcga gcaaatcagc cgagccagtg
     1801 gaactggaca cagccctgtt tccatgcttg gccactttca acccggcaga cgccagaaca
     1861 agagcagata acgtagaaag attgattgta ttgcttctgt ctccgcctgt cccgcaagta
     1921 tcgaggatgg cgtcttctga tcgaccggtc cacgcaactg ccgcttttct catggaacga
     1981 gcaaacgcct caatttcaac gggagtctcc cctttcatgg ccagggcggt cagcaaagcg
     2041 gccgtcgaga tttctccggc ttctcccgtc atgatggcag tcatcgcatg cgacgcttct
     2101 tctgcagtga gatcctgcct gcttactact ttctttaaat attcctgcat tacactttct
     2161 ccttcacgcg atacggcggc agaagcagct tatagtttgt aacaagataa cgaagcccag
     2221 aaagaatggt gatgatcgtt gtgatgagca tcaggtaata tggtagaaag ctggccagac
     2281 tgtaaaacca gtccgcatga tttcctgcca ttgcttcctg taaattgaac catgcgacat
     2341 caaacggccc aattccctgc gatcttcctt ctgcgtaaat tcgattgatt tcttttttat
     2401 ctccgtacga aaccagaagg aaactcatga gaatgatgac gatgctgaac atttgcagag
     2461 cagtcttcca tttgccaaaa cgagacgtgc gaagagactt acctttgcga attccaagat
     2521 aacgaagagc tgtgatgagc atatcacgcc ctacaatgca caaaaccatc catacctgaa
     2581 cctgttcggt aagaacgaca aacgtgataa acgcgccgag taccaggaac ttgtccgcaa
     2641 gcggatcgag gaactttcca aactctgttt gctggttcca tttacgcgcg aggtatccgt
     2701 ccacaagatc tgtaagagac gccagagcaa aaagaatgaa agcagtcaaa cgccaggtga
     2761 aagaaggctg caccagaaag taaatgaaga atggaacaga gatgacgcgc agtacggtta
     2821 gaaaattggg cagattcaag ttattacgcc tgtcagatcg tattccgtaa agccggtgat
     2881 ctcaacattt acaaggtcgc ccgaatggag caaccttgat ttaggctctg cgtatacgac
     2941 tccgtcagct tccggagcat cctgtggacg ccgcaaaacg acctcttctg cgctcgattc
     3001 gtccaccatg catgtaaaag tagtgccgac gagggagcgt aggtgctccg actgaacacc
     3061 cctgtgcacg ctctgcactc tgcgaattct atctgcaatg atttccgggg caatttgcgc
     3121 ggacatctct gccccgggag ttccttcctc gggagaatac gaaaacagcg ccagcttctc
     3181 cggcttcgcg gtggcaatga actgctctac cttttctaca tccgcatccg tttctcccgg
     3241 aaaaccaagt agaatcgaag tgcgaacttc gcatcctggc atgacatcgc gagcgagagc
     3301 aaacagatct gcgaatcttt ctgaacttcc aattctcctc atcgcgcgca gaacagactc
     3361 agatgcgtgc tgcaccggac tttctagata cgggaccact ttaggcatag agcgagttcg
     3421 cataccttcg aggattcgct ctgtctttcc gtcaggatac atatacagca tgcgaatcca
     3481 atgcagtcct tgtacctcat gaattctctc taccagatcc aggtatgctt ccggttttcc
     3541 gccgtacgaa ttggaatcct gactgaccag gaagacttcg cgcactccct gcgccgcaag
     3601 cctttcacat tcagcgatga tctcatctgc cggaatatct ctgaactttc cgcgaaagag
     3661 tggaatcgca cagaaagaac aaccgcgatc gcatccatct gaaattttca cgggcgcata
     3721 cggctttccc tgcgcctgca tttcgaaaaa aggagaaaca ggctcgagac ccacagacaa
     3781 cccgaatgcg tttcggatca gttcgcctgc cttgtgatag atcccggttc caaagctaaa
     3841 atcaatctct ggcaattcag cgcgcacggt gtccgcataa cgttccgtaa agcagcctac
     3901 aagaacgagc ttttgttttt tgtgtttctt tttgactttg cccgcgtcga gtactgtgcg
     3961 g
//
LOCUS       DAORFF010000370         3956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13817_length_3956_cov_3.366573, whole genome shotgun sequence.
ACCESSION   DAORFF010000370 DAORFF010000000
VERSION     DAORFF010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13817_length_3956_cov_3.366573"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PKK76_13735"
     CDS             <1..370
                     /locus_tag="PKK76_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69589.1"
                     /translation="ARANREDLRRNIDLRIDKFARETRERNNELSLLAEKVSISELRN
                     TVEYRKYISGQTALHDYLEEEIKLQQARADLLEKRVESILAVGQFEADIGLELDYLGL
                     IDLPENNPAPAPGQSAGITP"
     gene            367..1890
                     /locus_tag="PKK76_13740"
     CDS             367..1890
                     /locus_tag="PKK76_13740"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNL69590.1"
                     /translation="MKTRKFKTSMRFIRRKLNRLRSGRSASFQVHRRIAMRRLRSMPV
                     RARYAIIACSVLGITMAIYQLAKPKQPVDESMLMPEIRTETLKPGTLIHEIKNPGTVT
                     YLDKASVASRVLGRVQQIFVEQGSRVKKGEKLAQMETFEIILKQRQAQGSAAQARAQM
                     LLAQARYSAARREVEKQLTGIERTQSNIVESRAQLLTARQNLENKKELYDLGGVSGSE
                     LKQTYVTYLGAVSRLFQTRKEFQTQMIGFRDADLLGANISVPEAPAEKKRTFIDFNTQ
                     VDLQSFRASEAAYQSAQIELENTNLLLREATIVSPLDGVVAARSIEIGEEAKQNEPLF
                     TVVRMDKLVVETQLPEEEIKSIAPGSKTAFTADARAGKVFEGELYIISPVIDPKTRSI
                     PVRIRFDNNENLLAPGMFVRTRIRTREKKDALSIPETAVTNLRDQNSTRRGNVFVVKD
                     GLTMKREIVLGDRFGERYEVTAGLSAGEVLAIDGIDVLRDGARVKVKPELKAKVQEK"
     gene            1887..3416
                     /locus_tag="PKK76_13745"
     CDS             1887..3416
                     /locus_tag="PKK76_13745"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNL69591.1"
                     /translation="MNCPYFQSGLPQKKGVVFALFLAVSGIPSCSKNAANLPGILFAP
                     GSATRPSVVLSSPSALTPNVPSDIPVYVEFDREMRPNETENAFTLSGSAPTPGHFVWS
                     GKRLEYRLDGPLATGNSFVLVQSGSSISKDGYPMEVDYIVHFISGSSVTGPQVLSVAP
                     ANNSQNVATTSTIRFIFSRPMDIPSTQTAFSISPAAPGSFSWDPDQRGFTYTPTAALT
                     FATTYTVSLNVGARDTTGNPLAATSTTSFQAGTDFVPPTITNLFEVGNVTPLSPNQTG
                     IRKDSAFQIVFSEPVQFQTVQNAFSLVRQSDSSASSGTWQWNPTFTMATFTPGSPLEP
                     LHFYRASLGNTVLDIAGNSLQSAYHLNFYVDNSNGATNSDYLQAIGANQTAPLPLSSL
                     TISPAALSTVTLPGCLTGANMTIRVDFDHSLNLSTVAENLSISRTVGINPHVAQIIGL
                     SFATTTRPNDQLVIYLGDVGCNEYEVKIFGKAAGIRTAPVGAETGSWMQSDALIYMRV
                     Q"
     gene            3413..>3956
                     /locus_tag="PKK76_13750"
     CDS             3413..>3956
                     /locus_tag="PKK76_13750"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNL69592.1"
                     /translation="MIRRPLFALVILSAATVLSCAHKDPLQGLLLPGGGDLPKVVASY
                     PTNGLIGIDGSQGIWLLFDREMDQQKTQSAFTLGSAGGGTTGGFLWEGTRMIYNPRSG
                     LTGSDEFTMTLSKSAEASTGVNIQQDYIVRFFAQADTTHPTLTSSNPANGATNVATNQ
                     TITLNFSKPIDFSTISSGISI"
BASE COUNT         1057 a         1069 c          935 g          895 t
ORIGIN      
        1 cgcacgcgca aacagggaag atctgcggag aaacattgac cttcgcatag ataaattcgc
       61 ccgagaaacc cgcgaaagaa acaatgaact ttctcttctc gcagagaagg tttccatttc
      121 agaactgcga aacacagtag agtacagaaa atacatcagc gggcagacgg ccttgcacga
      181 ctatctcgaa gaagagatca agttacagca agcacgcgcg gatctgctcg agaagagagt
      241 ggaatccatt ctcgcagtcg gtcaattcga agcagatata gggctcgaac tggactactt
      301 aggactcatt gatctgcccg aaaacaatcc agctcccgct cccggtcaat ccgcaggaat
      361 cacaccatga aaacacgcaa attcaaaaca tcgatgcgat tcataagacg aaagctaaac
      421 cgcttacgat caggccggtc tgcaagcttt caggttcatc gtcgcatagc aatgcgtaga
      481 ttgcggtcca tgcccgtgcg cgcacgttat gcgatcatcg cctgcagcgt tctgggaatc
      541 accatggcta tctatcagct ggcaaaacca aaacagcccg tagacgaatc catgctcatg
      601 ccagaaatca gaacagaaac actgaaacca ggtactctga ttcacgaaat caagaatcca
      661 gggacagtga cgtatctgga caaggcttct gttgcatcga gagtacttgg ccgcgtgcag
      721 cagatcttcg tggaacaagg ttctcgcgtg aaaaaaggag aaaaactggc tcagatggaa
      781 acatttgaga tcatactgaa gcagcgtcag gcccagggaa gtgcagctca ggcaagagcg
      841 cagatgcttt tggcccaggc tcgctacagt gcagcgcgcc gagaggtaga aaagcagctg
      901 actggaattg aacgtactca atcgaacata gtagaatcgc gcgcccaact tctcaccgcc
      961 agacagaatc tcgaaaacaa gaaggaacta tacgacctgg gcggtgtctc aggctcagaa
     1021 ctcaagcaaa cgtatgtcac ctatctgggc gcagtatcca gactctttca gaccagaaag
     1081 gaatttcaga ctcaaatgat tggatttcgg gacgcagatt tgctgggtgc aaacatttca
     1141 gttccggaag cgcctgcaga aaagaaaagg acattcatag acttcaacac tcaggtcgac
     1201 ttgcagagct ttcgagcgtc agaagcggcc tatcaaagtg cgcagattga gctcgaaaac
     1261 acaaatctac tactgcgcga agctaccatt gtttccccgt tagacggcgt tgtggcagcc
     1321 agatccatag aaatcggaga agaagcaaaa caaaacgaac ctctctttac cgtggtgaga
     1381 atggacaaac tggtcgtgga gacccagctc ccggaagaag aaatcaaaag tattgcacct
     1441 ggatcaaaga ctgcattcac agcagacgcg cgtgcaggta aggtcttcga aggtgagctc
     1501 tacatcattt cgcccgtcat tgatcctaaa acaagaagca tcccggtgcg cattcgcttt
     1561 gataacaatg aaaatctcct cgctcccggt atgtttgttc gcaccagaat ccgcactcgc
     1621 gaaaagaaag atgctctgtc tattccggaa actgcagtca ccaacctgcg agatcaaaac
     1681 agcacgcgta gaggcaatgt attcgttgtg aaagacggtc tcacgatgaa gcgcgaaatt
     1741 gtgctcgggg atcgattcgg ggaaagatac gaagtcaccg ccggcctgag cgcgggagaa
     1801 gtacttgcca ttgatggaat cgatgttctc agagacggtg cgcgcgtcaa agtaaagcct
     1861 gaactgaaag caaaggtgca ggagaaatga actgccccta cttccagagt ggccttcctc
     1921 aaaagaaagg tgttgttttt gccctgtttt tagctgtttc tggcattcct tcctgcagca
     1981 agaatgcagc caatctccca gggattctct tcgcaccagg ttctgcgact agaccatccg
     2041 tcgttctttc cagtccttct gctttgacgc ctaacgttcc ttccgacatt cccgtttacg
     2101 tggaattcga ccgagaaatg cggcccaatg aaacagagaa tgcttttact ctgagcggat
     2161 ctgctcccac tccaggtcat ttcgtgtggt ccggaaagag actggagtat cgtctggacg
     2221 gacctcttgc cactggcaat tccttcgtcc tcgttcagag tggatcgtcc atttcaaagg
     2281 acggatatcc catggaagta gattacattg ttcatttcat ttccggttca tctgtaactg
     2341 gcccgcaggt gctgtccgtt gcgccggcga acaattcgca aaacgtggcc actacttcca
     2401 ccattcgatt catcttttcg cggcccatgg acattccttc cactcaaact gcgtttagca
     2461 tttctcccgc ggcgcctggc agtttctcct gggatccgga ccagcgcggt ttcacttata
     2521 cgccaactgc tgctttgact ttcgcaacca catacacagt gagcctgaat gtcggtgcgc
     2581 gcgacaccac cggaaatcct ctggcagcca cgagcactac aagcttccag gcgggaacag
     2641 actttgttcc gccaacgatc acaaacctgt ttgaggtggg caatgtaaca ccgctttctc
     2701 ccaaccagac tggaattcga aaagacagcg catttcaaat cgtatttagc gagcctgttc
     2761 agtttcaaac tgtgcaaaac gcattttctc ttgtgcgaca atctgactct tctgcgtcgt
     2821 ctggaacatg gcaatggaac ccaacattca caatggctac attcactccc ggctctccgc
     2881 ttgaaccgct ccatttctac agagcatctc ttgggaatac tgtactcgat atcgcgggaa
     2941 actccctgca aagcgcgtat cacctgaact tctatgtgga caacagcaac ggcgcgacca
     3001 actcagacta tctccaggca atcggagcca atcaaaccgc acctctgccg ctttcttctc
     3061 tgaccatttc tccggccgcc ctgagtacag taactctgcc tggatgtctt acaggcgcaa
     3121 acatgacgat tcgcgtagac ttcgatcatt ctctgaacct atcgaccgta gctgaaaatc
     3181 tttctatctc gcgcaccgtt ggaatcaatc cgcatgtagc acagatcatt gggctgagtt
     3241 ttgctacgac tacccgaccc aatgatcaac tcgtcatcta tctgggcgat gttggatgca
     3301 atgaatacga agtgaagatc ttcggcaaag cagcaggaat tcgcacggcg ccagtcggcg
     3361 ctgaaaccgg atcctggatg caatcagacg ctttaatcta tatgagggtc caatgatacg
     3421 ccgcccgtta ttcgcccttg tgattctatc tgccgcaacc gtcttatcct gcgcgcacaa
     3481 agacccgctg cagggcttac ttcttcccgg gggaggtgac cttccgaagg tagtggcgtc
     3541 ttacccgacg aatggcctga tcggcattga cggttcgcag ggaatctggc tcctttttga
     3601 tcgcgagatg gatcagcaaa aaacgcagtc cgccttcaca cttggaagtg cgggaggcgg
     3661 cacaaccggc ggctttctct gggaaggaac gcggatgatc tacaacccgc gatcggggct
     3721 taccggttca gacgaattca caatgactct ttcaaagtcg gcagaggcct ccacaggcgt
     3781 aaacatccag caggactaca ttgttcgctt cttcgcgcag gcagacacaa ctcatccaac
     3841 tcttacgtct tcgaatcctg caaacggtgc tacgaatgta gccacaaacc agacaatcac
     3901 tctcaatttt tctaaaccta tagatttctc aactatctca agcggaattt cgatct
//
LOCUS       DAORFF010000371         3946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13867_length_3946_cov_3.446929, whole genome shotgun sequence.
ACCESSION   DAORFF010000371 DAORFF010000000
VERSION     DAORFF010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13867_length_3946_cov_3.446929"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PKK76_13755"
     CDS             <1..696
                     /locus_tag="PKK76_13755"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNL69593.1"
                     /translation="GRLFKVIGRDEEFVLLEPDVDWSRESWEDFFERFGRVPIPPYFG
                     REDNEEDRIRYRTIYGQSGESLAAPTAGLHFTQEILDRLALKQIDVAALDLHVGFGTF
                     APLKPWNFEQHRLHLESFEIDDSCATAIENASRVIAVGTTTARALESVTRRHGKIQKA
                     NEDTDLFIFPPDSLKSVQGLITNFHLPSSSLFMLVCAFGGRQLMHRAYQFAIEQRFRF
                     FSYGDAMMILPGI"
     gene            733..1638
                     /locus_tag="PKK76_13760"
     CDS             733..1638
                     /locus_tag="PKK76_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69594.1"
                     /translation="MENFDKIGQTEMMKVPHFHLDEFQTRVLNNLHLEFFLKKCRFII
                     TPDYRYLLTGEADEKETGILDYKIPEKREALEKLKLLLIYDLSLYSALLETNSYYIAS
                     NGHLLICRFNTVKDHPEAFELKLYTISRDDLPENYKDKIYLGRDYISIKNLRKEHFGL
                     RYMRNSLIEQLMHLRARMMELLPEETREDLEEEFLSEVEELIGEFAEASNRIMEKFPV
                     EINTRTLERESLVQANAQFRNLKHILMEIEESLREMEQNLFEKDSPRAVRYVTKFKKD
                     VVNDINTIMFKINGRISDAVNGIHG"
     gene            complement(1607..3091)
                     /locus_tag="PKK76_13765"
     CDS             complement(1607..3091)
                     /locus_tag="PKK76_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69595.1"
                     /translation="MRPVYWIILVLTVFFAADSLIFRAGIYTLPNESAWETEPFYNLE
                     SAMHHLRTKSPDEFRILIVGSSLAMYGFLYERMEERMSGLAPGKKVKVFIVAHQGMHG
                     LELAALTDRYAALKPDLVILPVNMVDLRLERPILMSLMEDLGGTRRKEALDILYRDLA
                     GMYAVLPLAGQGMVEHYGRHLNVLQIGKVGLFGLCEGCRLKLFLPGPASTFFDNRFSR
                     GKSYDRFAGGRVEYNGEVAVTHRGHVPSNFAFRITPNQIARGMDLEIMQDGSVVAIRN
                     SLKSLKKEVQVSRGWVHFDLKGLGTVGDVLQVQISTSVYMDVFDDRYAARLTRNFATD
                     QPLLAGPRPRRREDELLDRMDDATYRKSFQIRNLAFEKPGYQYLKALYDAKKVWGTRA
                     FDPDLPAIAGVGDFVRRMPADTIVLNFPENPLTLSMYGQTRYYRDYVKYLQAIAAPGK
                     FVDASSVLPARKFYDYHHLTWYGAGEFSDYMSDWISREYHSRHH"
     gene            complement(3095..>3946)
                     /locus_tag="PKK76_13770"
     CDS             complement(3095..>3946)
                     /locus_tag="PKK76_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNL69596.1"
                     /translation="KLPHAMQQEVRNYYTYLWDTRRGYDESQVLVDLPKPIVEKVSMY
                     LLQQMIEKVPIFKGANEDLIRQIVIALKPEVYTPGDYIFRKGDMGGTMYFIGKGEVQV
                     VGDDGKTVFATLSEGNFFGEIALLMSMPRTASIRAVDFCDLYSLEKETFNGIVEHYPD
                     FSAHILELARARAREIGNRDTVTSTEAIHKPPRPARVVGLTASRVDDALRLSWEGVDT
                     AAVYQVIRKGEGRWTMVNGFVQNTVLIDHPLTGLSAYRVRAANESGPGDWSDVIEVAM
                     SATSKNP"
BASE COUNT         1051 a         1063 c          901 g          931 t
ORIGIN      
        1 ggtcgcctgt tcaaagtgat cggcagagac gaagaatttg ttttgctcga gccggatgtt
       61 gattggtcca gagaatcctg ggaagatttt tttgagcgat tcgggcgtgt gcctatccca
      121 ccctactttg ggcgcgaaga caacgaagaa gatagaattc gatacaggac catttacgga
      181 caatcaggcg aaagcctggc cgcccccaca gcaggtctgc atttcacaca ggaaattctg
      241 gatagactcg ctctaaaaca gatcgatgta gcggccctcg acttgcatgt gggatttggc
      301 acttttgctc ctctcaaacc ctggaacttt gaacaacaca ggcttcacct ggaatcattc
      361 gaaatagatg actcatgcgc cactgcaatc gagaatgcct cgcgagtcat tgcggtcggc
      421 acaacaaccg ctcgggctct tgaatcagtc accaggcgac acggcaaaat ccagaaggca
      481 aacgaagaca cggatctttt tatttttccg cccgattctc taaaatctgt ccagggactt
      541 atcacaaatt ttcatctccc ttcttcctct ctctttatgc tggtttgcgc ctttggcggc
      601 agacagctca tgcatcgcgc gtatcaattt gcgatagaac aacgatttcg ctttttcagt
      661 tacggagatg cgatgatgat actacccgga atttgattga ccggcgtcgt tgcgcaaggg
      721 acattgaaag gcatggaaaa cttcgacaag atcggtcaaa ccgagatgat gaaagttcct
      781 cattttcatc tagatgaatt ccagacgcgc gtgctcaata acctgcacct ggaattcttc
      841 ctgaagaaat gcagattcat catcacgcct gattaccgct atttgctgac tggagaagcg
      901 gatgaaaagg aaacaggcat cctggattac aaaatcccgg aaaagcgcga agccctcgag
      961 aaactaaagc tactcctgat ctacgatctc tccctctatt ccgccctgct cgaaactaat
     1021 tcgtactaca tagcttccaa cggacatctg cttatttgca ggttcaatac cgtgaaggat
     1081 catccggaag cgtttgagct caaactctat acaatcagcc gcgacgatct tccggaaaac
     1141 tacaaagata agatttacct gggccgcgac tacatttcca ttaagaacct gcgcaaagaa
     1201 cactttggcc tccgctacat gcggaactct ttgattgaac agctcatgca tttgcgcgcc
     1261 cgcatgatgg agctacttcc cgaagaaact cgggaagacc tggaagaaga gtttctatca
     1321 gaagtagaag aactcatagg tgaattcgca gaggcatcca acaggatcat ggaaaagttt
     1381 ccggtggaaa tcaacactcg tactctggag agagaatccc tggttcaggc aaacgctcaa
     1441 ttccgcaatc tcaagcacat tcttatggaa atagaagagt cccttcgcga aatggaacaa
     1501 aacctctttg aaaaagacag cccccgtgcg gttcgctatg tgaccaaatt caagaaagac
     1561 gtggtcaatg acatcaatac gatcatgttc aagatcaacg gtcgaatcag tgatgccgtg
     1621 aatggtattc acggctaatc caatcagaca tataatctga aaactcaccg gcgccgtacc
     1681 acgtaagatg gtgatagtcg tagaattttc gcgccggtaa aacggagctt gcgtccacga
     1741 attttcccgg agccgcaatc gcctgaagat acttcacata gtcgcgatag tagcgcgtct
     1801 gcccgtacat cgaaagagtc aaaggattct ccggaaagtt caagacgatt gtgtccgccg
     1861 gcattcgccg cacgaaatct ccaacacccg cgatagcagg aagatcagga tcaaacgccc
     1921 gagtgcccca taccttcttt gcatcgtata gagcctttaa atactgatag cctggtttct
     1981 caaatgccag attgcgaatt tgaaacgatt ttctgtatgt cgcgtcatcc attctatcca
     2041 gtagttcatc ttcgcggcga cgcggcctgg gccccgcaag caaaggctga tccgttgcga
     2101 aattgcgcgt gagtctggcc gcataacggt catcaaatac atccatgtac acagacgtag
     2161 aaatttgaac ctgcaaaaca tcgccgacgg ttcccaggcc tttcaaatca aaatgaaccc
     2221 agccccgcga gacctgaact tcctttttga gtgacttgag agaattccga atagcaacaa
     2281 ccgaaccatc ctgcatgatc tccagatcca ttcctctggc tatctggtta ggtgtgattc
     2341 taaaggcgaa gttcgaagga acatgcccgc gatgggtaac cgcaacctct ccattgtatt
     2401 ctactctacc gcccgcgaat cggtcgtagc tcttcccgcg cgaaaagcga ttgtcaaaga
     2461 aagtggaagc aggaccaggc agaaatagct tcaatctaca accttcgcac aggccgaaaa
     2521 gccccacctt gccgatctgc agcacattca ggtgcctgcc atagtgctcg accatcccct
     2581 ggcctgccag agggagcacc gcatacatgc ctgccagatc gcgatacagg atatccagcg
     2641 cttcctttct cctggtgcct cccagatcct ccatcaaact catgagaatg ggacgctcca
     2701 gcctcaggtc gaccatattg acgggcaaaa tcacgagatc cggtttcaat gccgcataac
     2761 ggtccgtaag ggccgccagt tccaatccat gcatcccctg atgagcaacg atgaacactt
     2821 tgactttctt tccgggcgca agcccggaca ttcgctcttc cattctctcg tacagaaatc
     2881 cgtacattgc cagagagctc cccacaatca ggattcgaaa ctcatccgga gatttagtcc
     2941 gcagatggtg cattgctgat tcaaggttgt agaatggttc cgtttcccac gcactttcat
     3001 tgggaagcgt gtagatgccc gcgcggaata tgagggaatc cgccgcaaaa aacacggtca
     3061 gtaccagaat gatccagtaa acgggacgca acaattacgg atttttagac gtggcggaca
     3121 ttgcaacttc aatcacgtcg ctccagtccc cgggacctga ttcgtttgct gcgcgaacac
     3181 gataggcgct gagccccgtc agtggatgat caatcaaaac cgtgttctgg acaaagccat
     3241 ttaccatcgt ccagcgaccc tcgcctttgc gaatcacctg ataaaccgcc gcagtgtcta
     3301 cgccttccca gctcaaacga agagcgtcat ccacgcggga tgcggtaagt cccacaacac
     3361 gcgcgggacg tggcggcttg tgaatcgcct cagtcgaagt aaccgtatcg cgatttccga
     3421 tttcgcgggc gcgcgcacgt gcaagctcca gaatgtgtgc ggagaaatcc ggatagtgct
     3481 ctacgattcc gttgaacgtt tctttttcca gagaatacaa atcgcaaaaa tcgacagcgc
     3541 gaatacttgc agtccggggc atactcatga gcagagcaat ttcaccaaag aagtttccct
     3601 cactcagtgt tgcaaatacg gttttgccgt cgtctcctac gacctgcacc tcacccttac
     3661 cgatgaagta catcgtccca cccatatctc cctttctaaa tatgtagtct cccggtgtgt
     3721 agacctctgg cttgagtgcg attacgatct ggcgaatcag gtcttcgttt gcacctttga
     3781 aaatcggaac cttctcaatc atctgctgaa gcagatacat ggagactttt tcaacaattg
     3841 gttttggtag atcgaccaga acctggcttt cgtcatagcc ccgtcgagta tcccagagat
     3901 aggtataata attgcgaact tcctgctgca tagcatgcgg aagttt
//
LOCUS       DAORFF010000372         3944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13878_length_3944_cov_3.204166, whole genome shotgun sequence.
ACCESSION   DAORFF010000372 DAORFF010000000
VERSION     DAORFF010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13878_length_3944_cov_3.204166"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PKK76_13775"
     CDS             complement(<1..525)
                     /locus_tag="PKK76_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNL69597.1"
                     /translation="MWFGAWGIGGWYWGPPRDQESTSAIEAALQSGIRAFDTAPVYGF
                     GHSEELIGQALQGRRHEAFIATKCSLVWDRPGDPFFTTVHGERSVDVRRNLSRSSILQ
                     ECEESLLRLRTDYIDLYQIHWPLSPLDEAAQALAELKGQGKIRFAGLCNTNVEQIIEW
                     KKLTGFHPDFVQERY"
     gene            complement(570..3167)
                     /locus_tag="PKK76_13780"
     CDS             complement(570..3167)
                     /locus_tag="PKK76_13780"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNL69598.1"
                     /translation="MPDIYSFSQKGCTADKEAWARSGKRGQRAFELAELNMPIVPGFV
                     LDSRLTPKLETMDLKDRLAQGIAEIEAGVGRKYGDPNNPLLLKIVCSSNLALSIYPTV
                     FNIGLSPTTIQGFINFLGEKAAWFEYCYLIRTAGVKIFDIPDSQFAEIEKKHPPSPEG
                     MKACAMEMVELVGRDKIPDDPMQQLEVLMKHAAKRYYDPDLDEDDNVAIMVQGIVFGN
                     MGEDSSVGMVYTRDIVTGEDKLSGNFMMNAYTMERTGTPIQDLPAAYLDDLRKICKAV
                     ERKFREIREIKFIVEKKKLWLINQTVVDGKSTQSHLRTLLDLMKENVVEESWVVQQIP
                     PGQLASLLQHIVEPASIASLPSASGGMTGSPGAAVGKVYFSADKVMEAHREAMLKGED
                     TRVILCVESSFAEDVKAIEVGQAVIAVEGGYSSHAPVVARSLGKVSILNQSIKIGENY
                     FELNGMRINEGDYVTLDCPVYKKPTIYAGKAKLINPDINTNGLVEFISVMKKFISDDF
                     VVRANADLGRDAKVARVMGAYGIGLCRTEHMFFQDDRIQRFREMILAKDYEERVKALN
                     ELRPMQKNDFYDLFKEMAPHHVTIRLLDAPLHEFLPRNDDAFADYTEHLRKKGVTPDE
                     KALRERIERLHEFNPMLGHRGCRVAITYPEIYQMQVRGIFEAACQLQKEGVTILPEIM
                     IPIVMNPNELTFIKNGKKIEGKVITGITDTARDVFKEYGVELEYKVGTMVELPAAAII
                     SDELARYAEFFSFGTNDLTQTTFGLSRDDVNSFFPAYTEFDILPNNPFQVLAPPVKEL
                     VKMSATRGKLTRPDLKLGLCGEHGADPANIAFCKEAGLHYVSCSPYSVPIALLAVAQM
                     NLKAGPGGK"
     gene            complement(3221..>3944)
                     /locus_tag="PKK76_13785"
     CDS             complement(3221..>3944)
                     /locus_tag="PKK76_13785"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNL69599.1"
                     /translation="SKFVTRSMMCVPLVVSRGVIGTVQVLNKKNGEFFNETDLRLFEV
                     MANQAAIAIENARLHEMATVDGTTGLYKKDYFIMRMEEEFRRSRNTGKPMSVIMSDID
                     HFKNVNTQFGHLGGDAALVELARVIRETVQEEGPEMMPGRYGGEEFCVLIPGEGPEQA
                     LGLAEKIRKRIEAEPIHIGDQVTHITISIGVSSWPTHTEHIQALDDFFRLADEALYIC
                     KDRGRNCSSLYDPEHKRPPAKN"
BASE COUNT          927 a         1024 c          969 g         1024 t
ORIGIN      
        1 gtatctttct tgaacaaaat caggatgaaa cccggtaagc tttttccact cgatgatctg
       61 ctcgacattg gtattgcaaa gacccgcaaa tcgaatcttg ccctgtccct tgagctccgc
      121 caatgcctga gctgcttcat ccagaggcga caggggccag tggatttgat agaggtcaat
      181 gtagtccgtg cgcagccgca aaagactctc ttcgcactcc tgcaaaatag aagagcgact
      241 tagattgcgc ctaacgtcga cagaacgctc gccgtgaacc gtagtgaaga atggatctcc
      301 tggcctatcc cagaccagag aacacttggt tgcgatgaat gcttcgtgtc gcctgccctg
      361 cagtgcctga ccgattagct cttcagaatg accgaatccg tatacagggg ctgtgtcaaa
      421 agcgcggatg ccagactgaa gggcggcttc gatggcagat gtggattcct ggtcgcgcgg
      481 tggaccccag taccaaccac cgatgcccca cgctccgaac cacacccggg gaagatcccc
      541 ggggagaaga caagacggaa ccgaaggaat tacttccctc cgggaccagc cttgaggttc
      601 atttgcgcca cagcaaggag cgcaatgggg acgctatacg gagaacaaga tacatagtgc
      661 agaccggctt ctttgcaaaa tgcaatgttg gcaggatctg ctccgtgttc cccgcagagg
      721 cccagcttca agtctggtct tgtgagtttg ccacgagtgg cactcatctt caccagttct
      781 ttgactggag gcgcaagaac ctggaacggg ttgttcggaa gaatgtcgaa ttctgtgtat
      841 gccgggaaga acgaattcac gtcatctcgt gacaaaccaa aggtagtctg tgtgaggtca
      901 ttggttccaa agctgaagaa ttcagcgtaa cgcgccagtt catcggagat gattgccgcc
      961 gccggaagct ccaccatggt ccctactttg tactccagtt ccacgccgta ctctttgaat
     1021 acgtcgcgag ccgtgtctgt gatgcccgtg atcacttttc cttcgatctt cttaccgttc
     1081 ttgatgaacg taagttcgtt tggattcatc acaattggaa tcatgatctc aggaagaatt
     1141 gtaacgcctt ctttctgcag ctggcatgct gcttcaaaga ttccgcgcac ttgcatctgg
     1201 tagatttccg gatacgtaat ggccactctg caaccgcgat gcccgagcat cgggttgaat
     1261 tcatgaagcc tttcaatacg ctcgcgaaga gctttttcat ccggagtcac acccttctta
     1321 cgaaggtgct ctgtgtagtc tgcgaacgca tcgtcgtttc gcggaaggaa ctcatgcaga
     1381 ggcgcatcga gaagacgaat ggttacatgg tgaggcgcca tttccttgaa cagatcgtag
     1441 aaatcgttct tctgcattgg gcgcagctca ttgagagctt tcacgcgttc ttcgtaatcc
     1501 tttgcaagga tcatctcgcg gaatctctgg atacgatcat cctggaagaa catatgctcc
     1561 gtacggcaga ggccaattcc gtaagcaccc atcacgcgcg ctactttagc atcgcggccc
     1621 aggtccgcat ttgcgcgaac cacgaagtca tcactaatga atttcttcat tacagaaatg
     1681 aactcaacga gaccgtttgt gttgatgtct ggattgatga gcttagcttt tcccgcatag
     1741 atggtaggct ttttgtaaac cgggcagtcg agtgttacgt agtcgccttc attgatgcgc
     1801 attccattga gctcgaagta gttctcacca atcttgatgc tctggttgag aatgcttacc
     1861 ttgccaaggg atcttgccac cacaggagcg tgagaagaat acccaccttc cacagcgatg
     1921 actgcctgac caacctcaat tgctttcacg tcctctgcaa aagaagactc cacgcataag
     1981 atcactcgag tgtcttcgcc cttgagcatt gcttcgcggt gcgcttccat cactttatca
     2041 gcggagaaat agacctttcc tacggccgcg ccaggagaac cagtcattcc acccgaagca
     2101 ctgggaagac ttgcaattga agctggttct acaatgtgct ggagcagact tgccagctgg
     2161 cctggaggaa tctgctgaac cacccacgat tcttccacaa cattctcttt catgagatcc
     2221 agaagagttc tcagatggga ttgagtggat ttgccatcta caaccgtctg gttgatcagc
     2281 catagtttct tcttttctac aatgaatttg atttcacgaa tctcgcggaa cttgcgctct
     2341 accgctttgc aaattttgcg aaggtcatcg agataggctg caggcagatc ctgaatcggt
     2401 gttccagtgc gctccatcgt gtatgcgttc atcatgaagt taccggacag cttgtcttct
     2461 ccagtaacga tgtcgcgtgt atacaccata ccaacgcttg agtcttcgcc catgttgccg
     2521 aacacgattc cctgcaccat gatggcgacg ttgtcgtctt cgtcgaggtc cggatcataa
     2581 tagcgcttgg cggcatgctt catgagcact tcgagctgct gcataggatc atctgggatt
     2641 ttgtcacgtc caacgagttc caccatttcc atggcgcacg ctttcattcc ttctggactt
     2701 ggcggatgtt tcttttcaat ttcagcgaac tgtgaatctg gaatgtcaaa aatcttcaca
     2761 ccagcagtgc gaatgagata gcaatattca aaccacgcgg ccttttctcc gaggaaattg
     2821 atgaagccct ggatggtagt aggcgaaagg ccaatgttaa acactgtagg ataaatagaa
     2881 agagcaaggt tagaactgca gacgattttg agaagaagtg gattgttggg atctccatac
     2941 tttctaccaa cacctgcttc gatttcagca atgccctgcg cgagacgatc cttgagatcc
     3001 atggtttcta actttggcgt gagtcttgaa tcgagcacga agccaggaac gattggcata
     3061 ttcagctctg cgagttcgaa ggccctctgt ccacgtttac cggaccgcgc ccaggcttct
     3121 ttgtccgctg tacagccttt ctggctaaat gaataaatat caggcatagg ttactccgtt
     3181 ggggaatcag attttcaatg caggccgatg tgtgccaatc ttaatttttg gcaggaggcc
     3241 gtttgtgctc aggatcgtac agggaggaac agtttctacc acgatcctta catatataga
     3301 gcgcttcgtc tgccagacgg aagaaatcgt ccagagcctg gatgtgctcg gtgtgtgtag
     3361 gccaggaaga caccccgatg gaaatggtaa tgtgagtgac ctgatcacca atgtggatgg
     3421 gttccgcttc aattctcttg cgtatcttct cagcaagtcc cagagcttgc tctggccctt
     3481 cgcctggaat gagaacacaa aattcctctc caccgtatct gcccggcatc atttccgggc
     3541 cttcttcctg gactgtctcc cgaatgacac gcgcaagttc caccagtgct gcgtctccgc
     3601 ccagatgacc aaactgagtg tttacatttt tgaagtggtc gatatcggac ataatgacac
     3661 tcatcggctt gccggtgttt cgcgatcgcc tgaattcttc ttccatgcgc atgatgaaat
     3721 agtccttctt ataaaggcca gtggttccgt ccacggttgc catttcatgc aggcgagcgt
     3781 tttcaatggc gatcgccgcc tggttggcca tgacttcgaa gagtctgaga tctgtttcgt
     3841 tgaagaactc tccgtttttc ttattgagca cctgtactgt tccaatgact ccgcgcgaaa
     3901 caacgagcgg aacgcacatc atcgagcgag ttacaaactt actg
//
LOCUS       DAORFF010000373         3933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_13935_length_3933_cov_4.083548, whole genome shotgun sequence.
ACCESSION   DAORFF010000373 DAORFF010000000
VERSION     DAORFF010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13935_length_3933_cov_4.083548"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..791
                     /locus_tag="PKK76_13790"
     CDS             15..791
                     /locus_tag="PKK76_13790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNL69600.1"
                     /translation="MEKPFNTKIFLILQCAAVAGLLAVIPYALALNPAATPDKLPLPL
                     PLIAAIQIGIQALMFAAAIAVGLFFSSRIGLGLPLLEAKLKGEPIAPNVRAILPFSAV
                     AGIVSSVLIVALDVYVFQPALGGAIGVKLPGRANPAAWKGFLASFYGGINEEILLRLC
                     LLSFLAWVGGFLLRGQNGKPAISAFWTANVVAAILFGLGHLPATSMILPVTPLVVLRA
                     VVLNGLAGIAFGYLYFRFGLESAMLAHFIADIVLHVLLAL"
     gene            complement(800..2839)
                     /locus_tag="PKK76_13795"
     CDS             complement(800..2839)
                     /locus_tag="PKK76_13795"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNL69601.1"
                     /translation="MSGQSTYVIWFATMDSLVVLLALIAILRLRSGLLRSIYLAFLAF
                     GALWVNLIYTVKWIAMPESIAVMLSRAIFVSILFTLCAFVCFMIAFCDLKEEFRKARF
                     FAFVGANALLILGTLLGYVENGVHMTATGLAPVYGPLHPVLVGSNLVWGAYVFWLVIR
                     EFRKSTDPIFRSQLRILFACCLFTFAWAGTMNGILPVLLGSSAFSHLGPLGFLVLYAG
                     IIWIILEERTLFIARDFRALLKLPAFQTQSNVMIMKQLFLDVEDAVTRPTSPFRKSYL
                     FHTGQGEADLTVSHGTGAAIQAPGALLPGNTEGFTDNLRALETQNRRLTFALLEAQSR
                     LRDPELGATIHSVPPRIPSSASGPYRLSDFVDEIEVNKRDMRESYGFPFPCFSRARVE
                     LLTRIRMYAPSLLTPLFVGPPASGRTTFSRVMHSLRSASSLREVSCASTDAGDLRPLV
                     REFLSPSCESTGILIRELHLLDPSGWPAIEQLLTEKRAGKWVYFTAEEEGLAELLSGA
                     GAHRATELLQLQLSVPALCSSREEMFFLALDLIRTRSRLLGRGIDAVSRTWMDRLISR
                     PWPGNYAEFAGVIDRALVESDSSIVDGSLPKLPAQATARKLEKAQAIPDLAHLTPLEN
                     AERRTIYEHLVANNFNQRKTSEEIGIRPNTLIAKMRKYEIAPPGGARPGRPRLNR"
     gene            complement(2836..>3933)
                     /locus_tag="PKK76_13800"
     CDS             complement(2836..>3933)
                     /locus_tag="PKK76_13800"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNL69602.1"
                     /translation="AEVIGFDASPYLSRRELKSYPRQTQFALVGLMLALGDAKLAALD
                     PYRTDVVLGAAQISSFYAIEEEIRKGSHALREHEPELDPFGVLKTTLSIPASAIAFRS
                     GVKGYVSTLTSACTSGIDAIGAAADRIRQGQADAVITGGVDTPITRIILNAFCAARML
                     STRNDEPQSALAPFDRSHTKSVLGEGAAILILEDKRRAIARGASIYCELGRFAQEPEN
                     TNELFSLDRSGTGWKRMLRNVLAEDGADSVEAINAHGPSDDLVDRVEADVLRDVFGAR
                     LHKIPAVSIKGGIGSSMAAAGAMQVASAALMLKTGIIPPTRNYSNPDPDCALNISAQV
                     RRMKRPLGSVLVNAHGIGGMNSAILLKRVKV"
BASE COUNT          983 a         1068 c          980 g          902 t
ORIGIN      
        1 cgcgcgcccc tgagatggaa aagcctttca atacaaaaat ctttctgatc ctacagtgcg
       61 ccgccgttgc aggattgctt gcagtgattc cttatgctct ggctttaaac cccgcggcca
      121 cgccggataa acttccgctt cccttgcctc tgatcgccgc aattcaaatc ggcattcagg
      181 cgcttatgtt cgccgctgcc attgcagttg ggctcttttt ttcaagcagg atcgggctcg
      241 ggctcccgtt gctcgaagcc aaactcaaag gcgaaccaat agcgcctaac gtcagggcga
      301 tccttccctt cagcgcagtt gcgggaattg tctcctccgt attgatcgtg gccctggatg
      361 tgtacgtgtt ccagcccgcc ctggggggcg ccatcggcgt gaaacttcca ggacgtgcaa
      421 atccggcggc atggaaagga ttcctcgcct ccttttacgg aggcatcaat gaggaaatcc
      481 tgctacgact ctgtctgctt tcttttctgg cctgggtggg tggattttta ctcaggggac
      541 agaacggcaa acctgcgatt tctgcattct ggaccgcgaa tgtagtcgcc gccatcctct
      601 ttggactcgg gcatttgcct gcaacatcca tgattcttcc cgtaacgcca ttggtcgttt
      661 tacgagccgt tgtcctcaat ggattagctg gaattgcctt tggctatttg tattttcgct
      721 tcgggcttga atcggccatg ctcgcacatt tcatcgcgga cattgtactg cacgtcttgc
      781 tcgctctcta acatcgaggt catcgattga ggcgcggtcg acccggccgc gcgcctccgg
      841 gcggtgcaat ctcatatttt cgcattttcg cgatgagggt gttgggtctg attccaattt
      901 cttcgcttgt cttgcgttga ttgaaattgt ttgccaccag gtgctcataa atggtgcgac
      961 gttctgcgtt ctcaagcggt gtcagatgcg ccagatccgg aattgcttgt gctttctcaa
     1021 gcttcctggc tgtcgcttgc gcaggcaatt taggaaggga tccatctaca atagaggaat
     1081 cgctttcaac taaggcacga tcgataacac ccgcaaactc ggcatagttg cctggccatg
     1141 gcctggagat caaccgatcc atccacgttc gactcacggc gtcaatgccg cgaccaagaa
     1201 gacgacttcg agtgcgaatc aaatcgagcg ccaaaaaaaa catctcttct cgcgaagaac
     1261 acaatgcagg aactgaaagc tgcaattgca ggagctctgt tgcgcgatgt gctcccgcac
     1321 cggagagcag ctcggccaat ccttcttcct ccgcggtgaa gtacacccac ttaccggcac
     1381 gtttttcagt taacagttgt tcaatggcag gccaacccga aggatcgagc agatgcaatt
     1441 ctcgaatcag aatccctgta ctttcacacg aaggagaaag aaactcgcgc accaatggac
     1501 gaagatcgcc ggcatccgta gacgcgcagg aaacttcgcg caatgatgaa gcggatcgca
     1561 gggaatgcat cactctggaa aaggtagttc ttccggacgc cggtggtcca acaaatagag
     1621 gagtcaagag agacggagca tacatgcgaa tgcgcgtcag cagctccacc cttgcgcgtg
     1681 aaaaacaagg aaaaggaaat ccataggact cgcgcatgtc gcgcttgttt acttcaattt
     1741 catcaacaaa atcagaaagt ctgtacggac ctgaagcgga agacggtatg cgtggcggca
     1801 cagaatgaat ggtggctccc agttccggat ctctcaatcg agactgagct tcgagcaatg
     1861 cgaacgtcag gcgtctgttc tgagtctcga gtgcacgtag attgtccgtg aatccctccg
     1921 tgttgcccgg caacagcgcg ccaggtgctt gaattgctgc tccagttccg tgcgaaacag
     1981 tcaaatcagc ttcaccctga cccgtgtgga ataaatagct ttttcgaaac ggagatgtgg
     2041 gcctggtgac tgcgtcctcc acatccagga aaagctgctt catgatcatc acgttggatt
     2101 gagtttggaa agcaggcagc ttcagaagtg cgcgaaagtc gcgggcaatg aacaatgtgc
     2161 gttcctcgag aataatccag ataatgcctg cgtaaagtac gaggaatcca agcgggccaa
     2221 gatgcgaaaa tgcagacgaa ccaagaagaa cgggcagaat cccgttcatt gtcccggccc
     2281 aggcaaatgt aaagagacag caggcaaaca gaatcctcag ctgagatcgg aagatcggat
     2341 ctgtgctttt cctgaattca cgaatcacaa gccagaatac gtaggccccc cataccagat
     2401 tggatccgac gagcaccgga tgcaaaggac cgtaaacagg agcaagtccc gtcgcagtca
     2461 tatgcacccc gttttccaca taaccgagca atgtcccgag gatcagaagg gcattggcac
     2521 caacgaaagc gaaaaagcgt gccttcctga attcttcttt caaatcacaa aacgcgatca
     2581 tgaagcacac aaaggcgcat aacgtgaaca atatggaaac aaagattgcg cgtgaaagca
     2641 tgaccgcgat agattccggc atcgcaatcc atttcacggt gtagatgaga ttcacccaga
     2701 gcgcaccaaa tgcaagaaac gcaaggtaaa tagatcgcag taagcctgat cgcaacctta
     2761 gaatcgcaat cagtgcaagc aaaacgacga gtgagtccat cgtcgcaaac cagatcacgt
     2821 aagtactctg cccgctcata ccttcaccct cttgagtaaa attgccgagt tcatccctcc
     2881 gattccgtgc gcatttacga gcacagatcc cagaggtcgt ttcatccgcc tgacttgcgc
     2941 cgaaatattc agtgcacaat caggatcagg attagaataa tttcttgtgg gagggatgat
     3001 cccagtcttg agcatcaaag cagcggaagc cacctgcatc gcacccgcgg cagccatgct
     3061 gcttccaatt ccacctttga tggaaacagc cggaatcttg tgcaagcgag caccaaaaac
     3121 atccctgagc acatctgcct ctacacgatc gactagatca tcgctcggtc catgagcatt
     3181 gatcgcttct actgaatctg cgccatcttc tgcgagaacg ttgcgcaaca ttctcttcca
     3241 gcctgttcca cttctatcga gtgaaaacaa ttcgtttgta ttctctggct cctgagcaaa
     3301 acgaccgagt tcacaataga tggaagcacc gcgagcgatc gcacgacgct tgtcctcaag
     3361 aatcagaatg gccgctcctt ctcccagaac ggatttggtg tgactacgat caaaaggagc
     3421 cagagcgctt tgcggttcat cattgcgagt agaaagcatt cttgccgcac aaaatgcatt
     3481 gagtatgatt ctggtaatgg gtgtgtccac tcctcctgtg atgactgcat ctgcctgtcc
     3541 ctgacgaatg cgatctgcag ccgcaccgat ggcgtcgatc ccactcgtgc acgcagaagt
     3601 cagggtggac acgtatccct tcactccact gcgaaatgca atcgcagaag caggaatgct
     3661 cagagtggtc tttagcacac caaatggatc gagttcaggc tcgtgttctc gcaatgcgtg
     3721 actgcctttc ctgatttctt cttctattgc atagaaggag gaaatctgtg ctgcgcccag
     3781 cactacatcc gttctatacg gatcgagcgc ggcgagcttc gcgtccccga gagcaagcat
     3841 cagcccaacc agtgcaaact gagtttgcct tggataggac ttcaattcgc ggcgagatag
     3901 ataaggggat gcatcaaagc caatcacttc cgc
//
LOCUS       DAORFF010000374         3919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14024_length_3919_cov_3.239130, whole genome shotgun sequence.
ACCESSION   DAORFF010000374 DAORFF010000000
VERSION     DAORFF010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14024_length_3919_cov_3.239130"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PKK76_13805"
     CDS             <1..985
                     /locus_tag="PKK76_13805"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL69603.1"
                     /translation="RAISGGLIRLLGYPAGHEITLQSLFDETTVAELFGRKLSAWQNG
                     LRFYRAKVKNAAGEQIFFDVSGYMEIAPGGRRFRVMLRELGPLDLALESGAFLENVHA
                     YSIAQRYISRDLITRARRAANMGNKQLLNETRVLTFLFADLVSFTAYSETKTPDEIVQ
                     MLNLSIGAASSNIIHYNGVVDKIMGDSVFAIFEQPLNAVLAAVEMQKQFQILNLFNVK
                     TNQPEIQVRVGIHTGECIQASLGTEDHMEWTAIGDAVNVASRLEKAAAPGTILASDHT
                     VELIREKVTTVKELDVAMKGKTLPMKAYYITRVTFDGPRGPITLGVDDDVF"
     gene            988..1884
                     /locus_tag="PKK76_13810"
     CDS             988..1884
                     /locus_tag="PKK76_13810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNL69604.1"
                     /translation="MAAKKSTRPAGKASSGPRVLPVPEQRKHVRKPGEIICYSWNVNG
                     IRAISRKGFREWFEDRNPDVLCVQETKAELEQVAKEKESQFLVDLEGYDSSWFSAEKK
                     GYSGVATFTRKSLEASSSKGFKKDGPDHFNNEGRVLVTTFGDFEIWNAYYPNGGRGPD
                     RVEYKLRFYDHCLDLWQKARKTKKIILTGDFNTAHKEIDLARPEENSTNTGFLPEERA
                     FLDKMIDAGYVDVFRHFEKGPNWFTYWDQVTMARNRNVGWRIDYFFVTEETMPLVTDA
                     WIEMDVPGSDHCPIGISLKSQS"
     gene            1953..2447
                     /locus_tag="PKK76_13815"
     CDS             1953..2447
                     /locus_tag="PKK76_13815"
                     /inference="COORDINATES: protein motif:HMM:NF013914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009523 - photosystem II [Evidence IEA];
                     GO_component: GO:0009654 - photosystem II oxygen evolving
                     complex [Evidence IEA];
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HNL69605.1"
                     /translation="MKKILTLLVLVLAVPVFAKPFNDKANKFKVSYPDGWKADTKAEH
                     FKVLFKSPDETANIGVAVVSVDKPVGAVDFLAEVEKQMGVENIMGQDERQLEDDANTQ
                     VGADDGAMGVFMRGKGESQLRQIMFIFTKGVKIYTVIITFNESDKETYRQLTKDVMNS
                     FQIQ"
     gene            complement(2410..>3919)
                     /locus_tag="PKK76_13820"
     CDS             complement(2410..>3919)
                     /locus_tag="PKK76_13820"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNL69606.1"
                     /translation="GTVTEMQEFLEFRKKRQKGGVTLMGGPVRFASRDIRESFEDLGG
                     TLIRGDVEVYAERIARDPRSMREAADSFTHTGERWNYEMVNRWAPAGAFLINLHPNFP
                     WLILELETYRGCTRDVFCSFCTEALYGKPVFRALPPIVEEVSELYKMGARHFRIGRQA
                     DLMTYLPDMNDFKNSFPRPVPASLEALYSGIRNAAPDLKMLHLDNINPGLIATFEKES
                     REIAHIICKYNTAGDTAAMGMESADIAVIEKNDLKSTEQETYRAIEIINEAGARREGG
                     VPKLLPGINLLHGLPGESEDTFKKNYEFLKRVIDNGLLLRRINIRQTVTYEHTKLDQI
                     LKERTARKKDGIQNRFMYYRDRIRREIDHPMLLLNFPPGTILRDVILESQNQGWYFGR
                     PLGSYPVTVKIPLDDRAMNAKPGTPCDVIITGAEERSLTALSYPVHLSLLGRKALETI
                     PGAGRQRALRLQSERPLNFQDFSAIFEGRPFGKESDYVFGVTGSEMSSSRPS"
BASE COUNT         1001 a          939 c          999 g          980 t
ORIGIN      
        1 ccgcgccatt tcaggcggac tcattcgact cctgggctat ccggccggac acgagatcac
       61 tcttcagagt ctttttgatg aaaccacagt tgcggagttg ttcggacgca aactttctgc
      121 ctggcaaaat ggtctcaggt tttatcgcgc gaaggtgaag aacgcagcgg gtgagcagat
      181 cttcttcgat gtatccggtt atatggagat tgcacctgga ggcagacgct ttcgcgtcat
      241 gcttcgcgaa cttggacctc tggatcttgc gctcgaaagc ggtgcttttc ttgagaacgt
      301 gcatgcatac agcattgcgc agagatacat ctcgcgcgat ttgatcactc gggccagacg
      361 cgcggccaac atgggcaaca aacagctgct caacgaaacg cgagttctta cttttctctt
      421 cgcggacctc gtgagcttta ccgcctattc tgaaacaaag acaccggatg agattgtcca
      481 gatgctcaat ctatcgatcg gtgcggcttc ctcaaatatc attcactaca atggtgtcgt
      541 ggataagatc atgggggatt ctgtgtttgc gatttttgag cagccactca atgcagttct
      601 tgccgctgtg gaaatgcaaa agcagtttca aattctgaat ttgttcaacg taaagacaaa
      661 ccagcctgag attcaggtgc gcgttggaat tcatactggc gaatgcattc aggcaagtct
      721 gggcacagaa gatcacatgg aatggactgc aatcggggat gcagtcaacg tagcttctcg
      781 ccttgagaag gcggccgcgc ctggaaccat tctcgcgtcc gatcatacag tggaactcat
      841 tcgcgaaaaa gtcacaaccg tgaaagaact ggatgttgcc atgaaaggga aaacactccc
      901 catgaaggcg tactacatca cacgcgtcac atttgacggc ccgcgcggtc cgatcactct
      961 gggcgtagac gacgacgttt tctaattatg gctgcgaaga aatccactcg ccctgcaggc
     1021 aaagcaagct ctggtccgag agtccttccc gttccggagc aaagaaaaca cgtgcgaaag
     1081 ccaggtgaga tcatctgcta ttcgtggaat gtaaacggaa ttcgcgccat ttccagaaaa
     1141 ggttttcgcg aatggttcga agatcgcaac cctgacgtac tgtgcgtgca agaaacaaaa
     1201 gcagaactag aacaggttgc caaagagaag gaatcacagt tcctcgtgga tctagaagga
     1261 tatgactcgt cctggttttc tgcggagaaa aaaggataca gcggagtggc cacgttcact
     1321 cgcaagtcac tggaagcgtc atcgtccaaa ggattcaaga aagatggacc ggatcatttc
     1381 aataatgagg gccgggttct cgttaccaca ttcggtgatt ttgaaatatg gaatgcgtac
     1441 tatccaaatg gtgggagagg gccggacaga gtagaataca agctccgttt ctatgaccac
     1501 tgtctggacc tatggcagaa agctcgcaaa acaaagaaga tcatactgac cggagatttc
     1561 aatacagctc acaaggaaat cgatcttgcg cgtccggaag aaaatagcac gaacactggc
     1621 tttttgcctg aggaaagggc gttcctcgac aagatgatcg atgcgggtta cgtggacgta
     1681 tttcgccatt ttgagaaggg accgaactgg tttacatact gggatcaggt gaccatggcc
     1741 aggaatcgaa atgtgggctg gcgaattgac tatttcttcg tgaccgagga aaccatgcca
     1801 ctggttacag atgcctggat tgaaatggac gttccaggaa gcgatcattg ccccattggg
     1861 atctcattga aatcgcagag ctgatgcgaa aaaggttgaa cgttccgggc ctgcgcgcgt
     1921 ctatcatcca aattcctttc cgggttaact caatgaaaaa aatcctcact cttctagttc
     1981 tcgtgctcgc agttcctgtc ttcgcgaaac ctttcaacga caaagcaaac aagttcaaag
     2041 tgagttatcc cgacggatgg aaagcagaca cgaaagcaga gcatttcaaa gtattgttta
     2101 aaagcccgga tgagactgcg aacattggtg ttgctgtggt ttctgtagat aagccagtag
     2161 gcgctgttga ttttctagca gaagtggaaa agcaaatggg tgtggaaaac atcatggggc
     2221 aggatgaacg gcagctggaa gatgatgcga acacccaggt gggcgcagac gatggtgcga
     2281 tgggagtctt catgcgcggc aaaggtgagt ctcagcttcg ccagatcatg ttcatcttca
     2341 caaaaggcgt gaagatttat actgtaatca ttacgttcaa tgaatctgac aaagaaacgt
     2401 accgtcaact cacgaaggac gtgatgaact catttcagat ccagtaactc cgaacacgta
     2461 gtcactctcc tttccgaagg gtcttccttc gaagatcgcg gagaaatcct gaaaattgag
     2521 agggcgctct gactgtagtc tgagcgctct ttgtcttcca gcacctggaa ttgtttctaa
     2581 agccttgcga ccgagcaggg aaagatgcac cggataagaa agcgcagtca ggcttctttc
     2641 ctctgctcca gtgatgatta catcgcacgg agtccccggt ttcgcattca tcgcgcggtc
     2701 atcgagaggg atttttactg ttacaggata agagccaagc ggacgaccaa aataccagcc
     2761 ctgattctgt gactcaagga tcacatctct caggatagtt cccgggggaa aattcaagag
     2821 aagcatagga tgatctattt cgcgtctaat ccgatcgcga tagtacatga atcgattctg
     2881 gattccatct ttctttcttg ctgttctttc cttcaggatt tgatcgagct ttgtatgttc
     2941 atacgtaact gtctgtctga tattgattct tcgaagcaga agcccattgt ctatgacacg
     3001 tttcaggaat tcatagttct tcttgaaagt gtcttcgctt tcgccgggca ggccgtgcaa
     3061 tagattgatt ccaggcaaca gtttgggaac gccaccttcg cgtctggctc cagcctcatt
     3121 gatgatttca atcgcgcggt aggtttcctg ttccgtgctt ttgagatcgt ttttttcaat
     3181 gactgcgata tccgcggatt ccattcccat cgcggccgtg tcaccggcag tgttgtactt
     3241 acaaatgatg tgagcgattt ctctggactc cttttcaaaa gtcgcgatga gccccgggtt
     3301 aatgttgtcc agatgaagca ttttcagatc gggcgccgca tttctaattc cgctgtagag
     3361 cgcttcgagg cttgcaggca cgggcctggg gaagctgttt ttgaaatcgt tcatgtcagg
     3421 caaataggtc atcaagtccg cctgacgtcc gattctgaag tggcgcgccc ccattttgta
     3481 gagctcgctt acctcttcta cgatgggagg aagagcgcga aacaccggtt ttccgtaaag
     3541 tgcttccgtg cagaaggaac agaatacatc gcgtgtgcaa cccctgtatg tttccagttc
     3601 aagaatgagc cagggaaaat taggatgcaa attgatcagg aacgctccgg ccggagccca
     3661 gcgattgacc atctcatagt tccatctttc tcctgtgtgc gtgaaagaat cggcagcttc
     3721 gcgcatggat ctgggatcgc gcgcgattct ttctgcatac acttcaacgt cgccgcgaat
     3781 gagagtacca cccaggtctt cgaagctctc tcgaatgtcc ctggaagcaa aacgaactgg
     3841 tccacccatc agagtgactc cgcctttttg cctcttcttg cgaaactcca gaaactcctg
     3901 catttctgtg actgtgcca
//
LOCUS       DAORFF010000375         3908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14082_length_3908_cov_3.848689, whole genome shotgun sequence.
ACCESSION   DAORFF010000375 DAORFF010000000
VERSION     DAORFF010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14082_length_3908_cov_3.848689"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1051
                     /locus_tag="PKK76_13825"
     CDS             152..1051
                     /locus_tag="PKK76_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69607.1"
                     /translation="MGRNASPWSVNLNELSFDAGILFCTAIIRFALFGDRPIAEALAS
                     WQLVVVYLLVCFAIPSYMGYVLGRYAKFTGRFTQRLILTIFSVVILTIFVFLTRIMLQ
                     LQDQNLMGFLLCSGTGLLLMGSIAGIVTMANAEDTEVVTNAFENPILSILGVTGSVIL
                     LFYLGIYKPFGITFEGGLGFLEFLAVFVGCPFAGVLFILVVGLAENAVMKAGGGEVLE
                     RMRHVLIPLFIAVSLMIFSNFAGHFLGQTSQSVGGSSLARILLFTFSGVLPFRIVLVF
                     MPPVRITNVIIGSVVLTASFISS"
     gene            complement(1080..2219)
                     /locus_tag="PKK76_13830"
     CDS             complement(1080..2219)
                     /locus_tag="PKK76_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69608.1"
                     /translation="METKSFLNLLLFNAGYASVCSSPPDRIVSASNWKNVQTMLQYEM
                     FKGSNAPASVTTFGSPGAATSKWTGGVLAANGKIYGMPRSAGTVLEIDPDTRTLLQFG
                     SIAGLNQYTGGVLAQNGKIYGIPYNATSVLEIDPIARTAVQFGTVSGATGKWEGGVLA
                     SNGKIYAMPSSVTTVLEIDPINRVVNQNAAVTGGSFYEIGSVLGPNGKVYGIPYSNAG
                     PAQLMELDPATGSTVLFGPSFPAFSFTSGVLAPNGMIYTIPYTNTAIYEIDPTKRTAT
                     AVTGVLVGGYSNGSLAPNGKIYMFPYDAPNTNVLEFDPNTKQMTSIGNNFAGGLANKY
                     EDGVMAPNGKIYTIPRDATNVAEISTGSVGTFCTGLLTSSYLNHY"
     gene            complement(2288..2854)
                     /locus_tag="PKK76_13835"
     CDS             complement(2288..2854)
                     /locus_tag="PKK76_13835"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 family protein"
                     /protein_id="HNL69609.1"
                     /translation="MNFFGKIKGRFLALTLAACILGGSIGCHGGFVLTSKWNGLITSI
                     GNKWIRWIVFLVLSFVYGFTILADAIVFNSVEFWTGSNPVALNYNEKGEYTRTLTSGS
                     ETAVMTYRNFGETLDVALFKNGVQTDSLRLIKSEPGVLYRQSNGQFEKIIVSEETRGN
                     LRIIRAGEKSAAVPAAVYQVELARSGSM"
     gene            complement(2851..3534)
                     /locus_tag="PKK76_13840"
     CDS             complement(2851..3534)
                     /locus_tag="PKK76_13840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNL69610.1"
                     /translation="MKLTLRLLSPALLFALAILIVIVYQVIRVGDKPGDAAFAIQESV
                     PLMILLAQLLWLLLLLYLKWRDSLTWTDLGWKKPQKSDSLIAIVSGLGLAAAYVRLLS
                     PAHTWLQRSIGDYVPAGGLISSLGSMKAVFFVANVLFAPFVEETIFRGYALPRLKERG
                     EFGVLWSCLFFGLIHWAGGFWYMILTGAVAGGLFAFIYLRNHSTTAAWLCHLSLNAAE
                     FAYVAWRDL"
     gene            complement(3568..>3908)
                     /locus_tag="PKK76_13845"
     CDS             complement(3568..>3908)
                     /locus_tag="PKK76_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNL69611.1"
                     /translation="KLVGIVTERDLALKVLAEGRGGDVPITEIYTPNPETAREGASVA
                     ECIVLLRDAHFRHLPIVDLEKKPVGIVSTRDFYKKLAEGMDRIFEETGFSLQIAEGED
                     PYTVILGPSV"
BASE COUNT          942 a          963 c          977 g         1026 t
ORIGIN      
        1 ctgtttctca aatatggagc aaacaaaaat gcgcgcaacc cgagcggtca aaccctgctc
       61 gatgtgctgc agaatgagct tagagacgaa gaatcgagtc tggcagacgg cgcacgtgag
      121 aacatagagg aaagcattcg agtgctcaag aatgggccgt aacgcttcgc catggtccgt
      181 gaacctgaac gagctttcgt ttgatgctgg aatcttgttc tgcactgcta tcatacgatt
      241 tgcactcttc ggagatcgac cgattgctga ggccctggcg tcgtggcagc ttgttgttgt
      301 ttacctgttg gtttgttttg ctataccgtc ttacatgggt tatgtgctcg gtcgatatgc
      361 aaaatttaca ggcaggttta cgcagagact gatcctcaca atattttctg tcgttattct
      421 gactattttc gtttttctta cgcgaatcat gttgcagctt caagatcaaa atctgatggg
      481 attcctgctc tgttccggga ctgggctgtt gctgatgggt tcgatcgccg ggatcgtcac
      541 gatggcaaat gcagaagata cggaagtagt caccaatgcg tttgaaaacc ccatactttc
      601 catactcggc gtgactggtt ctgtgattct tcttttctat ctgggtattt acaaaccctt
      661 cgggattact ttcgaaggag ggttggggtt tctagaattt ctggctgtgt tcgttggctg
      721 ccctttcgcg ggcgttctct tcattctagt agtcgggctt gctgaaaacg cggtcatgaa
      781 agcgggcggc ggggaggtgc ttgaacggat gcgccatgtg ctcattcctc tttttattgc
      841 agtttcactt atgatcttca gtaacttcgc aggtcacttc ctgggacaga ccagtcaatc
      901 agtcggagga tcatcgcttg cacgcatttt acttttcacc ttttcggggg tccttccctt
      961 tcggatcgta cttgttttca tgcctcctgt tcgaatcaca aacgtgatca ttggcagtgt
     1021 ggttttgacg gcatccttta tcagttcgta aggattcaag aaaccaggac gttaggcgac
     1081 taatagtggt tcaagtagga agaagtaagc aatccggtgc aaaacgtgcc gactgaccct
     1141 gtcgaaattt ctgcaacatt ggttgcgtct cgcggaatgg tgtagatctt cccattcggt
     1201 gccatcacac catcttcata tttgtttgcc aggccgcctg cgaagttatt tccaattgaa
     1261 gtcatttgct ttgtgttggg atcaaactca agtacgtttg tattgggtgc atcgtaaggg
     1321 aacatgtaga ttttcccgtt aggcgccagc gaaccattcg agtaccctcc caccagaacc
     1381 ccggtcactg cggttgcagt tctttttgtc ggatcaattt cgtatatcgc tgtgtttgtg
     1441 tatggaatgg tatagatcat tccgttaggt gcaagcactc cacttgtgaa agaaaatgct
     1501 ggaaacgaag gcccgaacag aacagtggat ccagtagcag gatcgagttc catcaattgc
     1561 gcgggccctg cgtttgaata tggaattccg tacactttcc cgtttggccc gagcacggaa
     1621 cctatttcat aaaagcttcc gcctgtgacg gctgcattct gattcaccac gcggttgatt
     1681 ggatcgattt cgagtactgt ggttacagag gaaggcatcg catagatctt tccattggat
     1741 gcaagaactc ctccttccca ttttcctgtg gctccggata cagtgccgaa ttgaacggca
     1801 gtgcgcgcga ttggatcaat ttccagaacg ctcgtcgcgt tgtacggaat tccatagatc
     1861 tttccgtttt gagcgagtac ccctccggta tactggttga ggccggcaat gctcccaaac
     1921 tgcagcaagg tgcgggtgtc cggatcgatt tcaagaacgg tgcccgcgct gcgcggcatt
     1981 ccgtaaattt ttccgtttgc cgcgagtacg ccgccagtcc atttcgaagt cgcagctccc
     2041 gggctgccaa acgtagtgac agacgccgga gcattcgatc ccttaaacat ttcgtactgc
     2101 aacatggtct ggacgttctt ccagttcgaa gcggagacaa ttcgatccgg tggcgaagag
     2161 cagacgctcg catagccagc gttaaatagg aggaggttga gaaagctttt ggtttccacg
     2221 ccattcgccg gctgctggca acttgccagc accagaaggc aaacagaaat cgatttattc
     2281 ctctgcatca catggagcca gagcgcgcca attccacctg ataaactgcc gcaggaaccg
     2341 cagcagactt ttcgcctgca cgaatgattc gcagatttcc tctggtttct tcgctaacga
     2401 ttattttttc aaactgaccg ttggactggc gataaagaac acctggttca gatttgatga
     2461 gcctcagaga atccgtttgc acaccattct tgaaaagagc aacgtccaga gtttcgccga
     2521 agttcctata ggtcatcact gcagtctctg atccagatgt aagggtgcga gtgtattcgc
     2581 ctttctcgtt gtagttgagt gccactggat tggatccagt ccagaattct acggagttaa
     2641 atacgatggc gtctgcgagg atcgtgaacc catagacgaa tgatagcacc agaaatacga
     2701 tccatcggat ccatttgttt ccgatgcttg taatcagccc gttccattta gaagtgagaa
     2761 caaaaccgcc gtggcaacca atgcttccgc ctaaaatgca ggcggccaga gtgagtgcga
     2821 gaaagcgtcc ctttatcttg ccaaagaaat tcatagatcc ctccaggcga cgtatgcaaa
     2881 ttccgccgcg ttgagcgata ggtgacagag ccaggccgca gtcgtcgagt gatttcgtaa
     2941 atagatgaac gcaaacaatc cacctgccac agcacccgtg agaatcatgt accagaatcc
     3001 acctgcccaa tgaatcagcc caaaaaagag gcaactccag aggacgccga actcaccgcg
     3061 ttctttcagt ctcgggagag cgtatccccg gaagattgtt tcttctacaa aaggggcaaa
     3121 caggacattg gccacgaaga acactgcttt catggatcca agggaggaga tgagaccacc
     3181 tgccggaaca tagtcaccga tcgacctttg cagccaggta tgcgccggag ataaaagccg
     3241 tacgtaagct gccgcaaggc ccaggccgga cacaatcgcg atcagagagt ccgatttttg
     3301 tggcttcttc catccgagat ctgtccaggt aagggagtct cgccatttga ggtagagcaa
     3361 cagcagaagc cacaaaagtt gtgccagaag aatcatcaga ggaacggatt cctggattgc
     3421 gaatgcagcg tctcctggtt tgtcccccac tcttattact tgataaacta taactattaa
     3481 gattgccagc gcaaacaaaa gtgcaggcga aagaagacgt agagtgagtt tcatgctatg
     3541 gccgtgtata cgtgattctt gatttgctca gacagaagga ccaagaatca cggtgtaagg
     3601 atcttcccct tccgcaattt gcagactgaa tccggtctct tcgaagatgc gatccattcc
     3661 ttccgcaagc ttcttgtaaa agtctcgagt ggaaacaatt cccaccggct ttttttcaag
     3721 atccacgata ggcaaatggc gaaagtgagc gtctctgaga agcacaatgc attccgcaac
     3781 agaggcgcct tcgcgggctg tttcaggatt gggagtatag atttccgtaa tgggcacatc
     3841 tccacctcga ccttctgcga gcaccttgag cgccaggtct ctttccgtta caatgccgac
     3901 taactttc
//
LOCUS       DAORFF010000376         3897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14140_length_3897_cov_3.686882, whole genome shotgun sequence.
ACCESSION   DAORFF010000376 DAORFF010000000
VERSION     DAORFF010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14140_length_3897_cov_3.686882"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="PKK76_13850"
     CDS             complement(<1..168)
                     /locus_tag="PKK76_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016750399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL69612.1"
                     /translation="MKIYVVDDDNSIRASLKEVLQDEDFDVEDFATGKNMLKALSRER
                     PALILLDVWMGK"
     gene            complement(165..1637)
                     /locus_tag="PKK76_13855"
     CDS             complement(165..1637)
                     /locus_tag="PKK76_13855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL69613.1"
                     /translation="MTTTPWQTEERRIYLRDIYFLIFIVILSIGLAELFLVGEDAEPV
                     DRILSYLMVFVPLGAINLLAHYYYRNRRIRATGTLRTSLRYRLSLAFMVVAIIPSIPI
                     FLISSNVAEHLVGAFFEVDVRDAIDRSQKIIRETHIAESHPEYKDSIKKIDRVGVSLD
                     NREQWKERIPARIRMGLGLIFLFMICASLVGAVWLASSISTPIVTIAAATREVTDGRL
                     DTRIEMEAEGELGILIDSFNQMTVELQSLRTRLLHSQRVAAWQEVAKRLAHEIKNPLT
                     PIQLSADRMVRRLERPEKGDLQQIVSSGARTIREQVQILKTMVEEFADFARMPEPRPV
                     LQSLDTIAGEIGALYQSVPGVKMEIRLAGNLPDIELDKNMVVGMINNLVKNAIEAIAA
                     KPDEPGRIVLSTALHTQAGRKFVMLRVEDSGPGIDEGMRDRVFEPYYSTKGEHGSGLG
                     LALVERTVLDHNARITVSRSALGGAEFRILFPIPMEGRKS"
     gene            complement(1634..1894)
                     /locus_tag="PKK76_13860"
     CDS             complement(1634..1894)
                     /locus_tag="PKK76_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNL69614.1"
                     /translation="MEKRNITVTSREGMHTRPAAVFVREAAKFKSEVMLTSGDTTVNG
                     KSIMGILMLALTSGTTVTLQVDGPDESSAADALARVLSGDSG"
     gene            complement(1894..2838)
                     /gene="hprK"
                     /locus_tag="PKK76_13865"
     CDS             complement(1894..2838)
                     /gene="hprK"
                     /locus_tag="PKK76_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNL69615.1"
                     /translation="MKQIPVASLIGAESGLELEAVSGKAGLSRTISSFDINRPGLALA
                     GFYKNFADDRIQVFGKGEFAFIEECAVADQAEIAKNFFRFELPGLVLTHGNQPPICFV
                     EMSEKHSIPILTTRQSTHSFIMQFTHWMIEELAPSTSVHGVLVEVFGVGILLMGQSGI
                     GKSETALELVERGHRLVADDAVHVRCVEGSDLYGQASELLQYHMEIRGLGIINVKDLF
                     GVGAIRGRKRIELVIELEEWNAEKEYERLGLDDELVEILGVPVPRITVPVRPGRNIPV
                     LIETAAMNHRSKKMGYHAARVLSERLKEQMEKKRYSNH"
     gene            complement(2846..3157)
                     /gene="raiA"
                     /locus_tag="PKK76_13870"
     CDS             complement(2846..3157)
                     /gene="raiA"
                     /locus_tag="PKK76_13870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNL69616.1"
                     /translation="MEITYHFHHLESSDGLKDYAKKKIEKLAAHFNAFHSAMVRFRVE
                     TKIEHSVELTLNGDGVQFIATEKGADMYAIIDALEEKLGKQIRKHKEKHLGASHRGER
                     G"
     gene            complement(3169..>3897)
                     /locus_tag="PKK76_13875"
     CDS             complement(3169..>3897)
                     /locus_tag="PKK76_13875"
                     /inference="COORDINATES: protein motif:HMM:NF016439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HNL69617.1"
                     /translation="KVIRGLEPYPGTLFSPPRHDYIVPDMSVQEVDGTFEVFVQDEWI
                     PELKINDRYKNLMRKETRVSGEDREYLQSRLNSAAWLIKSIKQRRETMIQVMRSIIRH
                     QKAFFEQGPGHLRPLTLREIAADVELHESTISRITTNKYVQTRWGIFELKHFFSSSLR
                     NSEGGDDHSARNIKDRIARLVSEETDPLSDQEIVHILKKEGVEIARRTVAKYRKILRI
                     LPADRRKKLQGLKQKNQPAEKARS"
BASE COUNT          894 a         1028 c          914 g         1061 t
ORIGIN      
        1 ctttcccatc cacacatcaa gcaagataag agcggggcgt tcgcgcgaga gtgctttgag
       61 catgtttttg cccgttgcaa aatcctcaac gtcgaagtcc tcgtcctgga gaacttcctt
      121 gagggaagcg cgaatgctgt tgtcgtcgtc aacgacgtag atcttcatga cttgcgacct
      181 tccatcggga tggggaatag aatgcgaaac tctgctccac ccagagcgct tcttgaaacc
      241 gtaatacgcg cattgtgatc gagaaccgtt ctttccacaa gtgcaagccc cagcccactt
      301 ccatgctccc ccttcgtaga gtaataaggt tcaaaaacgc ggtctctcat cccttcgtca
      361 atcccgggtc cggaatcttc cactcgaagc atcacaaatt ttctgccggc ctgagtgtgc
      421 agtgccgtgg aaagtacaat gcggccgggt tcatctggtt tagccgcgat ggcctcaatt
      481 gcatttttta ccaggttgtt gatcatacca acgaccatgt tcttatcgag ctcaatatcc
      541 ggcagatttc cggcaagacg aatctccatc ttcaccccgg gaacactctg atagagagca
      601 ccaatttctc ccgcgatcgt gtctagagac tgcagaaccg gcctgggttc cggcatgcgg
      661 gcgaaatccg caaattcttc caccattgtt tttagtatct gcacctgctc gcgaatagtt
      721 cgcgctccgc tcgaaacgat ttgctgcaga tctcctttct caggtctttc gagtctacga
      781 accattctat ctgcggagag ttgaatcgga gtgagaggat ttttgatttc gtgtgcaagc
      841 ctcttggcga cttcctgcca ggcggccacc ctctggctgt ggagcaatcg cgtacgaagg
      901 ctctgcaatt ctacagtcat ttgattgaag ctatcaatga gaatccccag ttctccttct
      961 gcttccattt ctatgcgagt atcgagtctg ccgtctgtga cttcgcgcgt tgcggcagca
     1021 atggtaacga tgggtgtgga gatggaactt gccagccaca cagcacccac cagcgaagcg
     1081 cagatcatga acaaaaagat caaacccaga cccatacgga tgcgagcggg aattcgttcc
     1141 ttccattgtt cgcggttgtc aagggaaaca cccacgcgat caatcttctt gattgaatct
     1201 ttgtattccg ggtgtgactc tgcgatgtga gtttctcgaa tgattttctg agagcgatcg
     1261 atcgcatccc tcacgtccac ttcaaagaat gcaccaacca ggtgttccgc tacgttagac
     1321 gaaatcagaa agattggaat agaaggaatg atggccacta ccatgaaggc caggctcaag
     1381 cgatagcgaa ggcttgtgcg gagagtaccg gtggcgcgaa ttcgtctgtt gcgataataa
     1441 taatgcgcaa gtaaattgat agctccaagt ggaacgaaaa ccatcaaata agataggatg
     1501 cggtctactg gttctgcatc ctccccgacc aaaaacagtt cggccaggcc aatactcaaa
     1561 atgacaatga atatgagaaa gtaaatgtcg cgcagataga ttctgcgctc ttctgtctgc
     1621 cagggcgttg tggtcatccg ctgtcgcccg aaagcacacg ggcaagagcg tctgcggccg
     1681 acgattcatc tgggccgtcc acctggagag tgaccgtagt ccctgaagtc aaagcaagca
     1741 tcaaaattcc catgatgctt tttccattca cggtagtatc tccgctcgtg agcatgactt
     1801 cacttttgaa ttttgcggct tcacgaacaa acacagctgc gggacgcgtg tgcatccctt
     1861 ctcgactcgt tacagtaata tttctctttt ccatcagtga ttcgaatatc ttttcttttc
     1921 catttgttct ttgagtcttt cagaaagaac ccgcgcggca tggtaaccca tcttcttgga
     1981 tctatggttc atggctgccg tttcaatcag cactggaatg tttcgcccgg gtctgacagg
     2041 cacagtgatg cgtggtacgg gaactcccag gatttccaca agctcgtcgt cgagacccag
     2101 acgttcgtat tctttttcgg cgttccattc ctcgagttca atcacaagct cgattctctt
     2161 tcttcctcga atcgctccaa cgccaaacaa atccttcacg ttgatgattc ccagcccccg
     2221 aatctccatg tggtattgca gaagctcgct tgcctgtccg taaagatccg aaccttccac
     2281 gcatctcaca tgaaccgcgt cgtctgcaac cagtctatgg cctctttcca cgagttcgag
     2341 cgccgtttcc gattttccaa ttccgctctg tcccatgagc aagatgccca ctccaaagac
     2401 ttccacaagc actccatgca cggaagtaga aggcgcaagt tcctcgatca tccaatgagt
     2461 gaattgcatg ataaaagagt gagtgctctg tctcgtagtc agtatgggga tagaatgttt
     2521 ctcggacatt tctacgaagc aaatcggcgg ttgattgccg tgcgtcagga ccagccccgg
     2581 gagttcgaag cgaaagaagt tctttgctat ctccgcctgg tctgcaaccg cgcattcttc
     2641 tatgaaagca aactcgcctt tgccaaaaac ctgaatacga tcatctgcaa aattcttgta
     2701 gaatccggcc agagcaaggc ccggccgatt gatgtcaaaa ctggaaatgg ttcggctgag
     2761 gccggcctta ccggaaactg cttcgagctc gaggccgctc tccgcgccaa tcagggatgc
     2821 tactggaatt tgcttcaaac gagaatcagc ctctttcgcc tctatgcgat gcgcccaggt
     2881 gcttctcttt gtgcttgcga atttgttttc cgagtttttc ttccagtgcg tcaatgatcg
     2941 catacatgtc cgcgcccttt tctgttgcaa tgaactgcac tccgtcaccg ttcaaagtta
     3001 attcgacgga atgctcaatc ttggtttcga cacgaaaacg aaccattgct gaatgaaatg
     3061 cattgaagtg cgccgcaagt ttctcgattt ttttctttgc gtagtccttg agcccgtccg
     3121 agctctcgag gtggtgaaag tgataggtaa tttccatttt ctgctccttc agctccgcgc
     3181 tttttccgca ggctggtttt tttgtttgag gccctggagt ttctttcttc tatccgcggg
     3241 cagtattcgc aggatctttc tgtattttgc tacagttctt cgtgcgattt caacaccctc
     3301 cttttttaat atgtgcacaa tctcttgatc ggacagcggg tctgtttctt ccgaaacaag
     3361 cctggcaatt cgatccttga tgtttcgcgc cgaatgatca tccccaccct ctgaatttcg
     3421 cagcgaactt gaaaagaagt gcttcaattc aaagattccc cagcgggttt gaacatactt
     3481 gtttgtagtg attcttgaaa tagtggattc gtgaagttcc acgtcagctg cgatttcgcg
     3541 cagggtgagt ggtcggaggt ggcctggccc ttgctcaaag aaagctttct ggtggcgaat
     3601 gatcgaacgc atgacctgaa tcatggtttc acgtctctgt ttgatgctct tgatcaacca
     3661 tgcggcagaa ttcagtctgg actgaagata ctcccgatct tctcctgaaa cccttgtctc
     3721 tttgcgcatc aggtttttgt aacgatcgtt gatcttcagt tctggaatcc attcgtcctg
     3781 aacgaacact tcgaaagttc cgtccacttc ttgaacggac atatccggca cgatgtagtc
     3841 atggcgagga ggcgaaaata gagtgcctgg ataaggttca aggccgcgga tcacttt
//
LOCUS       DAORFF010000377         3893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14171_length_3893_cov_3.052110, whole genome shotgun sequence.
ACCESSION   DAORFF010000377 DAORFF010000000
VERSION     DAORFF010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14171_length_3893_cov_3.052110"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKK76_13880"
     CDS             complement(<1..315)
                     /locus_tag="PKK76_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69618.1"
                     /translation="MKKINLAKVVLMCALVATASACKKEATEVVATGTVLALKGVISE
                     TLAASDMKKAGIAFAAFDATWENVEDGVKAASPNAYKDIENGMDEVNIAIKKNDAAKL
                     KEA"
     gene            complement(312..1775)
                     /locus_tag="PKK76_13885"
     CDS             complement(312..1775)
                     /locus_tag="PKK76_13885"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HNL69619.1"
                     /translation="MRTVCLAFWVVSALAWPAFASPVEDMRELDSILARSEEALARGD
                     LKAARAQYELFGDRWFAVEDGVKHQSRPAYKAIESAMGGVRFAFTEPGRPDEIRRSLG
                     ALRNANAAFLAGKFGASQEDSGGKQIAQATVPELLVILSQVSESIERKDAARAKLEFD
                     SFQSGWPDVEGFIGTKSRDDYVLIENHITTAQAALRSTPADYQIASRSVEILKDKLRL
                     YSASQRYTMLDAATILIREGLEALLVIVALAAFLEKSGSANRKSWIWGGSLVGVVVSI
                     LSGVLIHRLFAGFVTNSNRELVEGLTGLFAAAMLLYVSYWLHSKSSLASWQNYIKDQV
                     TSALARGSLLSLGFLAFLAVFREGAETALFYIGIAPAISPVDLFSGIGIGVAVLTLAA
                     ILMLFVGLRIPLRPFFLFTGLLIYYLGFKFVGSGVHALQIANVLSVNSSEHLWSIPSF
                     GVYPTWETSVPQLMLLILLATAVLYSRMRKNTSGGTT"
     gene            1911..3113
                     /locus_tag="PKK76_13890"
     CDS             1911..3113
                     /locus_tag="PKK76_13890"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNL69620.1"
                     /translation="MSLKRFPLICASVIAIVATCSVESSPYDVALSSYSPVTPLREVR
                     ALWIVRDSIKTTESIRAIVESMRRNKFNTAIVQVRGRGDAYYRSEFVPVAAGIAPGLD
                     PLAEFIRVAKPYGISVHAWINVFLGADRESLRTAPKNHLAFAHRDWFLRDRSGRSMLT
                     YSAREMRAADVEGAFLDPSNQDVRRYNVQVVHELLSKYPVDGIHLDYIRYPSSQTGTP
                     FDFGINSLLPTDPRKAPDILAMRKARAENVTQMVSEIREEIRRIGPSRILSAAVWPNK
                     MKVEERVFQMWPEWLRSRLIDYAFLMAYYDTETLHDQRVSQFFDPEINNRMIIGVGIY
                     KNPAPKVALHQLTSARAMGAAGICYFHAEWFLSKNAIEKEKKYQLPEVFDTWKDPGFT
                     PARTSSIP"
     gene            complement(3116..>3893)
                     /locus_tag="PKK76_13895"
     CDS             complement(3116..>3893)
                     /locus_tag="PKK76_13895"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNL69621.1"
                     /translation="EIMPETINTDLIEENPFLYGKRLFFVRYPFGKPELAHIYYSDLI
                     ESGWSEARPLFSFSAITPGVFRGRFFFATRAQSGKYQIQSIPLDQILSPDAKNLIRAE
                     PGLDPQSDEASYAESRDGTVVFARRSPTGDYDLFSMNQDPWESSENFSLTSILFESSR
                     SDVLPESSPILDRLAEYLKRTQAKVFVTGHTDRTGDPQANLQLSRDRALAVKRELVNR
                     GVSPDRVKTDGKGQTEPVDPGETEEAYAKNRRTEFKILRD"
BASE COUNT         1000 a         1044 c          942 g          907 t
ORIGIN      
        1 tgcttccttg agttttgccg catcgttctt cttgattgcg atgtttactt catccatgcc
       61 gttttcgata tccttatagg cattaggcga tgctgccttg accccatcct ccacattctc
      121 ccaggttgcg tcaaaggcgg caaaagcaat gcccgctttc ttcatatcac tggcggccag
      181 agtctctgaa atcactcctt tgagcgcaag cacagtgcca gtcgccacaa cctctgtcgc
      241 ctctttcttg caggcagatg cggttgccac gagcgcgcac atcaaaacta cttttgccaa
      301 atttattttc ttcatgtggt tcctcctgaa gtattctttc tcattcgcga atatagtaca
      361 gccgtagcga gcaggatcaa aagcattaac tgaggaacgc ttgtctccca ggtagggtaa
      421 acgccaaaag acggaatgga ccacaggtgc tcggaagaat tcacggacag cacatttgcg
      481 atctgcagtg catgcactcc actgcctaca aacttgaatc caaggtagta gatcaggagc
      541 cccgtaaaaa ggaaaaacgg acgcagcgga attcgcagac cgacaaagag catcagaata
      601 gcggctaacg tcaacacggc gactccaatt ccaatgccag aaaaaagatc cacagggcta
      661 atggcgggag caatcccaat atagaaaaga gccgtctcag ctccttcgcg aaacaccgca
      721 agaaacgcca gaaatccaag agagaggaga cttcctcgtg caagagcgga ggttacctga
      781 tctttgatat aattctgcca cgacgccagg gatgacttgc tatgaagcca gtaactgaca
      841 tacaaaagca tggcggccgc aaacaaccct gtcaggcctt ctacaagttc acgatttgaa
      901 ttcgtaacaa agccagcgaa aagacgatgg atcaagacac cggacaaaat tgaaacgacg
      961 actcccacca atgaaccgcc ccagatccag gacttccgat tcgctgaacc actcttctcc
     1021 aggaaggctg ccagtgccac aatgacgagg agggcttcga gaccttcgcg gatcaagatc
     1081 gtggcggcgt cgagcatcgt ataacgttgc gacgcagagt atagtcgcag tttgtctttg
     1141 agtatttcca ccgaacggct tgctatctga taatcggcgg gcgtggaacg aagcgccgcc
     1201 tgcgcggtcg ttatgtgatt ctcaatcaga acgtagtcat cacgagattt ggttccaatg
     1261 aagccctcca catccggcca accagattga aacgaatcaa attctaattt tgccctggcc
     1321 gcgtcctttc gctctatgct ttcagatact tgcgaaagaa tcaccagcag ttcgggcacc
     1381 gtagcctgag cgatttgttt gcccccggaa tcttcttgcg atgcgccaaa ctttcctgcg
     1441 aggaaagcag cgtttgcatt gcgaagagct cccagcgagc gacggatttc atctggtcgc
     1501 ccgggttcgg taaacgcaaa gcgcacaccc cccatcgcac tttctatcgc tttgtatgca
     1561 ggacgcgatt gatgcttcac tccgtcctct actgcgaacc agcgatcgcc aaacaactcg
     1621 tactgcgccc gggctgcctt caggtctcct ctggccagtg cttcctcaga tcgcgccagg
     1681 atggaatcca gctcacgcat atcctccaca ggggaagcaa acgcgggcca cgccagagca
     1741 gaaacaaccc agaaggccag acaaacagtg cgcatgagac aagattctga aatggggcac
     1801 ggcaggcaag ccattctgat attaagaatc agtctcaatt agatcgggat tgcggtccgt
     1861 tcccaattct ggttgaatcc tgcggccacc agggttgcct ggagtgcccc atgagcttga
     1921 aacgattccc actgatttgc gcaagcgtca tagccattgt cgcaacctgc agcgtggaaa
     1981 gcagtccgta tgatgtggcg ctgagttctt attcacccgt aacgccactt cgggaagttc
     2041 gtgctctctg gatcgtacgc gattctatca aaaccacaga gtccattcgt gccatcgtgg
     2101 aatcgatgcg aagaaacaag ttcaataccg cgatcgtcca ggtaagaggc cgcggcgacg
     2161 catactatcg gtccgagttc gttccggtcg cagccggaat tgcaccaggg cttgaccctc
     2221 tggcagaatt cattcgcgtt gcaaaacctt acggcatatc tgttcatgcg tggatcaatg
     2281 tttttctggg agcagacaga gaaagcctgc gcactgcgcc caaaaatcac ctggcctttg
     2341 cacacaggga ctggtttctt cgggatcgca gcggcagatc catgctcacc tattcagcaa
     2401 gggaaatgcg cgcagcggat gtggaaggtg catttcttga tccatctaac caggacgtta
     2461 ggcgatacaa tgtgcaagtc gtccacgagc ttctgtcaaa gtatccggta gatggaattc
     2521 atctggatta cattcgatac ccttcttccc agacgggcac tccctttgac ttcggaatca
     2581 attcactctt gcccactgat cccaggaaag cacctgacat tctggccatg cgtaaggctc
     2641 gagctgagaa cgtaacgcag atggtatctg aaattcgaga agagatccga cgcatcggac
     2701 catcgcgcat tctatctgcc gccgtatggc ctaacaagat gaaggtggaa gagagagtat
     2761 ttcaaatgtg gccggaatgg cttcgatcga gactcattga ctatgcgttc ctcatggctt
     2821 actacgacac agagactcta cacgaccaac gagtatcgca attcttcgat ccagaaatca
     2881 acaacagaat gatcatcggt gttggaattt acaaaaatcc tgcgcctaaa gtagctcttc
     2941 atcaactgac gagcgcccgt gcgatgggag cggccgggat ctgctacttt catgcagaat
     3001 ggtttttgag taagaacgca attgaaaaag aaaagaaata ccagcttccc gaagtattcg
     3061 acacgtggaa agatccaggc tttactcctg cgcgcacatc gagcattccc tgattctaat
     3121 cacgcaagat cttaaattcc gttcgcctgt tcttcgcata cgcttcttct gtttctcctg
     3181 gatccactgg ctccgtctgc cctttgccgt ccgtcttcac tctgtcaggt gaaacaccac
     3241 gatttactag ttcgcgcttc accgcgaggg cccgatcacg agaaagctgt agatttgctt
     3301 gtggatctcc cgtgcgatcg gtatggcccg tgacaaagac cttcgcttgc gtcctcttga
     3361 gatattccgc caggcgatcc aggatcggcg atgattctgg aaggacatcg ctgcgcgagc
     3421 tctcaaagag tatgctcgtt aaagaaaaat tctccgaaga ttcccatgga tcctgattca
     3481 tgctaaaaag atcatagtcg cctgtgggcg agcgtcttgc gaaaacaacg gttccatctc
     3541 tggattctgc ataggacgct tcgtccgatt gcggatcgag gcccggctct gcgcgtatca
     3601 gatttttcgc atcgggggat agaatctgat cgagaggaat ggactgtatt tggtactttc
     3661 cgctttgtgc tctcgtggca aaaaagaacc ggccgcgaaa cacacccgga gtgatggcgc
     3721 taaaagaaaa aagaggacga gcctcagacc agccactctc aattaaatcc gaatagtaaa
     3781 tatgtgcaag ttctggcttg ccgaatggat atcgcacaaa gaataaacgc ttaccgtaca
     3841 gaaacgggtt ttcttctatc agatccgtat tgattgtttc aggcatgatt tct
//
LOCUS       DAORFF010000378         3885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14227_length_3885_cov_3.676240, whole genome shotgun sequence.
ACCESSION   DAORFF010000378 DAORFF010000000
VERSION     DAORFF010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14227_length_3885_cov_3.676240"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PKK76_13900"
     CDS             <1..581
                     /locus_tag="PKK76_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide lyase"
                     /protein_id="HNL69622.1"
                     /translation="NSHLATISNAATSCTGNCNHRGYPTIQLHKTAAGGMSGVVFTEF
                     YVYVTMTINDGQWLSLATLTPDSSDAWSRTVLVNVGNLGRTGGGNYMHLMHVPNQGES
                     GWTYQAGSTNGGTQFPQNQWVKVSICLDLNPATGWAKAWMNDVPVSAAPVSGGCGVLE
                     QAHFGLYAPPAISSGVIYNDDLQIREVSVCPK"
     gene            complement(583..987)
                     /locus_tag="PKK76_13905"
     CDS             complement(583..987)
                     /locus_tag="PKK76_13905"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HNL69623.1"
                     /translation="MTESGMQRARVIKWPHKETPTKEAVYKELEMHGFQVYDLQTIPP
                     WCERSLHTHDYPEIRGAVAGVTTFHFPEGPVTIEAGDILFIPAGVPHSLRTHNGNEFT
                     AYKGSINGIRKVTELGDGKNSVEYLAKLKDAQ"
     gene            1093..3219
                     /gene="scpA"
                     /locus_tag="PKK76_13910"
     CDS             1093..3219
                     /gene="scpA"
                     /locus_tag="PKK76_13910"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNL69624.1"
                     /translation="MANFADWQKQLAKDLKGTTDQDLTTMTPEGIPLKPLYTAADLAA
                     TDQEAVGSLPGLYPFLRGPYTSMYSHRPWTIRQYAGFSTAEESNAFYKRNLAMGQKGL
                     SVAFDLATHRGYDSDHPRVKGDVGKAGVAIDSVEDMKILFSDIPLADISVSMTMNGAV
                     IPVLACFIVAGEEQGVGPDKLAGTIQNDILKEFMVRNTYIYPPDPSMRIVSDIIEYTA
                     HRMPKFNSISISGYHMQEAGATCELELAFTIADGLEYVKAALARGLDIDEFAPRLSFF
                     FAIGMNFYMEIAKLRAARVLWARHMKVFNPKSEKSLLLRTHCQTSGVSLTEQDPYNNV
                     IRTAIEAMAAVFGGTQSLHTNALDEAIALPTDFSARIARNTQLIIAEETGIPRVIDPW
                     GGSYFMEKLTQDLVDRAEALIKEVNELGGMTKAILSGMPKRKIEECATRHQAQVDKGD
                     KTIVGVNKYRAKTQDPMEILEIDNADVLRKQIARLEKIRSSRDAGAVKAALGAIEEAA
                     KSGKGNLLELAVNAARLRATVGEISDAMETSFGRYAAPIQAISGVYAAHFAENEEWKL
                     IRTEVEKFAKDKGRRPRILIAKMGQDGHDRGAHVVATSLSDLGFDVDIGALFMTPQEV
                     AKSAVENDVHVVGVSSLAAGHKTLVPELIQELKKQNAGDIKVVCGGVIPQQDYDFLKK
                     AGVAAVFGPGSSILSTAKEILKAISQ"
     gene            3216..>3885
                     /gene="rpiA"
                     /locus_tag="PKK76_13915"
     CDS             3216..>3885
                     /gene="rpiA"
                     /locus_tag="PKK76_13915"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNL69625.1"
                     /translation="MKQADANQDLKKAAGIRAASFVQSGMRVGLGTGSTAAFLIEELG
                     RRKREENLQIVCTATSFSSQVLGEKAGLTVLPIDQFDDLDISIDGADEIDPRLYLIKG
                     GGAAHTREKLVHAMSRRFVVIADSSKRVQKLGVKFAVPVEVIPCSARYAMRRLMALGA
                     THCEIRMSKSGKDGPVVTDNANFILDSTMDVADPISMETAINCIPGVLENGIFASVRP
                     VQAVI"
BASE COUNT          968 a          948 c         1029 g          940 t
ORIGIN      
        1 gcaattctca tctagcaact atttccaacg ctgcaacaag ctgcactgga aactgcaatc
       61 atagaggata cccaaccatt cagcttcata agaccgccgc aggcggaatg tcaggtgttg
      121 tttttactga gttctatgtg tatgttacta tgaccatcaa cgacggacag tggctgagcc
      181 tggcaactct tactcctgat tcttcagatg cctggagtcg caccgtgctc gtgaatgttg
      241 gaaacctggg acgcacaggg ggcggcaatt acatgcactt gatgcacgta ccaaaccagg
      301 gggagagcgg ctggacgtat caggcaggct ctacgaacgg tggaacgcag tttccgcaga
      361 accagtgggt caaggtttcc atctgtctgg acttaaatcc ggcgacgggc tgggccaaag
      421 catggatgaa tgatgtgcct gtgtctgcag caccagtctc gggaggttgc ggagtactcg
      481 aacaggctca ttttggattg tatgctccgc ccgcgatttc aagtggagtc atatacaacg
      541 atgatcttca gatcagagaa gtctctgtct gtccaaagtg aatcactgcg cgtcttttag
      601 ttttgcgaga tactctacgc tgttttttcc gtcacccagt tccgtgactt tgcgaatacc
      661 gttgatcgag cctttgtatg ctgtgaattc atttccgttg tgcgttctaa gggaatgcgg
      721 aactccagct ggaataaaaa gtatgtctcc tgcttcaatg gtaaccggac cttccggaaa
      781 atgaaacgtg gttacaccag ccactgctcc gcgaatttca gggtagtcat gagtgtgcag
      841 gctacgctcg caccagggcg gaatcgtctg cagatcgtat acctggaatc cgtgcatttc
      901 tagttctttg tataccgctt cttttgttgg agtttctttg tgcggccatt tgatgacccg
      961 ggctctttgc attcctgatt ctgtcacatt tgcgcctcca ggtctttgac gcccgtcagg
     1021 tctcgatgat gactttcgtc acacgtgctg ttttcggctt ttcttgcgca cgcgcgggag
     1081 aatccctgca tcatggcgaa tttcgcagac tggcaaaagc aacttgcaaa agatctaaaa
     1141 ggcacaaccg accaggacct gaccaccatg actccggaag gcattccgct gaagcccctt
     1201 tacactgcgg cggatctggc ggccacagat caggaagctg taggatctct acccgggctt
     1261 tatccctttc ttcgcggccc atacacttcg atgtacagcc atcgcccatg gacgattcgc
     1321 cagtatgcag gcttctccac tgcagaagaa tcgaacgcat tctataaaag aaacctggcg
     1381 atggggcaga agggtctgtc cgtggcgttt gacctggcca ctcatcgtgg ttacgactca
     1441 gatcatccca gagtgaaagg agatgttgga aaggcaggcg ttgcgatcga ttcagtcgag
     1501 gatatgaaga tcctcttcag cgacattcct ctggcagaca tttcagtttc tatgaccatg
     1561 aatggtgcgg tgattccagt tctcgcctgt ttcattgtcg caggagaaga gcagggagtg
     1621 ggtccggaca aactcgccgg cacaattcaa aacgatatcc tcaaagaatt catggtgcgc
     1681 aacacctaca tctatccacc cgatccttcc atgcgaatcg tgtctgatat cattgaatac
     1741 acagcgcatc gcatgccaaa gtttaattcg attagcattt caggttacca catgcaggaa
     1801 gcaggagcga cctgtgaact ggaactcgcc ttcacgatcg cagatggtct tgagtatgta
     1861 aaagcagcgc tcgcgcgcgg attggatata gacgagtttg cacccaggct gagtttcttt
     1921 tttgcaatcg gaatgaactt ttacatggag attgcgaaac tgcgtgcggc acgcgttctc
     1981 tgggcgcgcc acatgaaagt attcaacccg aaatcagaaa aatctcttct ccttcgcacg
     2041 cattgtcaga caagcggggt aagcctcaca gaacaggatc cgtacaacaa tgtgatccgc
     2101 accgcaatcg aagccatggc cgcagtgttt ggcggcacac agagtcttca caccaacgct
     2161 ctagacgaag caatcgctct tcccactgat tttagcgctc gaattgcgcg aaacacgcag
     2221 ctcatcattg cagaggaaac tggaattcct cgcgtgattg atccatgggg cggcagttac
     2281 ttcatggaaa aactcacgca ggacctggtc gatcgcgccg aggctttgat caaagaagta
     2341 aacgaactcg ggggaatgac aaaggcgatt ctttccggaa tgccaaagag aaagattgaa
     2401 gaatgtgcca ctcggcatca ggcccaggtg gataaaggcg ataaaactat cgtaggtgtg
     2461 aacaagtatc gcgcgaagac gcaggatcca atggagattc ttgaaataga caatgcagat
     2521 gttctgcgca agcagattgc tcgtctggaa aagattcgtt cttcgcgcga tgcaggagct
     2581 gtcaaagcag cactcggtgc catcgaagaa gcagctaaat ccggcaaagg caatcttctc
     2641 gaactcgctg tcaacgccgc caggcttcgt gctaccgtgg gagaaatttc agacgccatg
     2701 gaaacatctt ttggacgtta tgcggcaccc attcaggcta tttctggagt gtacgcggcg
     2761 cactttgctg aaaatgaaga gtggaaactc attcgcaccg aagtggagaa atttgcaaaa
     2821 gacaaagggc gcagaccccg tattctcatt gcgaagatgg gccaggacgg tcacgatcgt
     2881 ggagctcatg tagtggccac ttctctgtct gaccttggat ttgatgtgga cattggagcc
     2941 ttgttcatga ctccgcagga agtggccaag tcagctgtgg aaaatgacgt tcatgtggta
     3001 ggagtgtcgt ctctggccgc cggtcacaaa acgctcgttc cagaattgat tcaggaacta
     3061 aaaaagcaaa acgccggcga catcaaggta gtctgtggag gcgtcattcc acagcaggat
     3121 tatgatttct tgaaaaaggc gggagtggcc gctgtgtttg gacctggatc gagcattctt
     3181 tccaccgcca aagaaatact caaagcgatt tctcaatgaa gcaggcagac gcaaatcaag
     3241 atcttaagaa ggcggcagga attcgcgctg cctctttcgt gcagtctggc atgagagtcg
     3301 gtcttggcac aggctccact gcagcctttc tgatagaaga gctcggtcgc cgcaaaagag
     3361 aggagaactt gcagatcgtg tgcacggcca cttcgttttc cagtcaggtg ctgggagaga
     3421 aggcgggact cacagttctg cccatagatc agtttgatga cctcgatatt tctatcgacg
     3481 gggcagatga aattgatcct cgcctgtatt tgatcaaagg cggaggtgcc gctcatacgc
     3541 gcgagaaact ggttcatgca atgagtcgcc gcttcgttgt gattgcagat tcgtctaaac
     3601 gagtgcaaaa gcttggagtg aaatttgccg tgccggtgga agtgattccg tgttcggctc
     3661 gctatgcgat gcgaagattg atggcgcttg gcgcaaccca ctgtgaaatt cggatgagca
     3721 agtctggaaa agatggcccg gttgtaacag acaatgcgaa ttttattctg gattcgacca
     3781 tggatgtggc agatccgatt tccatggaaa cagcaatcaa ctgcattcct ggcgtacttg
     3841 aaaatggcat cttcgcatcc gtgcgaccag tgcaggcagt gattg
//
LOCUS       DAORFF010000379         3878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14267_length_3878_cov_4.127125, whole genome shotgun sequence.
ACCESSION   DAORFF010000379 DAORFF010000000
VERSION     DAORFF010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14267_length_3878_cov_4.127125"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="PKK76_13920"
     CDS             <1..940
                     /locus_tag="PKK76_13920"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNL69626.1"
                     /translation="HLVAGPIIRHSDFFYQLGDIKADQDKIVRGMFLILTGLVKKVAI
                     ADNILPLFSRVFLKPDEYSGAANAMAAVSFSIWVFCDFSGYTDLARGMSLLLGIELPE
                     NFRAPFLSRSVRELWRNWHSTLGTWMRDYVYIPLGGSRRGEVRSQLNLLTTFTLGGLW
                     HGAHYPYIIWGFFHGLMLVLERFLGLGLDKVLPTKIQLPRALDIVRTGLQVVFVFILF
                     CIGAVFFNSPTVTSAFTMLGRAFTWVPGSPLQATETLSYFVIAGFLLNGIQLIEKWPS
                     LKLSHAATIAGVYGFIVMFLLAKYAPGGRDFLYFQF"
     gene            949..2082
                     /locus_tag="PKK76_13925"
     CDS             949..2082
                     /locus_tag="PKK76_13925"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNL69627.1"
                     /translation="MRQFKILLVPLALFAVVFALDKVFLLPIVRENFVSWKKIEPPFY
                     EARRDLYDLYKRSYQADKARGKKIGAIFGTSRSAEFNTDGIDQILHDSRTYNFGAPFA
                     CPSFFYYWYSQMKADGILPDYVILETDPLLFNERAIDYSLSYSYDSSFVIKHLELGRA
                     RGGDPWSARPGFSFDEGETFFLKRLFALYRYPPDLGVYKDNNQDLPLPLPDGTIRIWK
                     GKEFRAELLKWMGSINELKLGGIPNMLSIQLPPEKLKGDAANMAAIHLTGCSPSRTQI
                     AFYRLLTKSLAEKRIQTVVYWPVVSDAFQEKIDDARLPDFVQRARVEQEIKKVDPAGI
                     IQLIYPAELKCRTFVDSVHLSGSCFPELEKILIEKGLPAQKRK"
     gene            complement(2067..3452)
                     /locus_tag="PKK76_13930"
     CDS             complement(2067..3452)
                     /locus_tag="PKK76_13930"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNL69628.1"
                     /translation="MLFNSFDFLVFFFVFYAVYWQLQGEWRRRWLILSSMAFYAWWDF
                     LYLFHFTAVVLISYYCYLRVREKHSKSWFYVGLALNIANLVFFKYVATTLQWLGGVTG
                     LAAFSHAGEALHFGFPLAISFYTFQLIAILVDAWRGDIDEEIGFQNFLQYSFFFPHLI
                     AGPILRHRDYFPQIGTERFDRTQSTAGIYLILFGLLKKVVIADGISSIIDPVYSNPAK
                     FTTVGAVAALFGFALQIYADFSGYTDIARGLAKMLGYEIPENFRAPYFSYTFADLWRK
                     WHITLSMWLRDYIFFPLGGSRTSPLRSHLNVLITMILAGLWHGHSYMFFFWGVYIGAL
                     MTIERLLGIGKREFSFPAKAALWVVMFTLWSSSALFFRGHDFTSAMAMIHTLSGDAAA
                     GAVMPPNWTRFPGLVALSILVHVVQYQRERLAAPLLKYGRYLIPVIAVALFYMLVRLD
                     RSTVQFIYFRF"
     gene            complement(3455..>3878)
                     /locus_tag="PKK76_13935"
     CDS             complement(3455..>3878)
                     /locus_tag="PKK76_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HNL69629.1"
                     /translation="IARRLEKEPGIGFALARPAGSFREQLARSACKLADSLKSPAIVV
                     MTRRGILAEQVASFRPSTSIIYAFTNMSSSRRKLWLARGVVPFLMDFSNDPEKNIRAA
                     FEKLRARNRVLTGDEIVVVSDVAAGSERFTSIQIRRLD"
BASE COUNT         1043 a          998 c          933 g          904 t
ORIGIN      
        1 gcaccttgtg gctgggccga tcattcgcca ttcagacttc ttctatcaac tgggagacat
       61 caaagcagat caggataaaa ttgttcgcgg gatgtttctg attctcacgg gtctggtaaa
      121 aaaagtggcg attgcggaca acatacttcc cttgttctct cgcgtcttcc tcaaaccaga
      181 cgaatactct ggagcggcaa acgcaatggc cgcggttagc ttttctatct gggtcttctg
      241 cgatttctcc ggctatacgg atctagcgcg cggcatgtcg ctactcctcg ggattgaact
      301 tccggaaaat tttcgagcac cattcttatc acgcagcgtg cgagaactct ggagaaactg
      361 gcattccact ctcgggacgt ggatgcgcga ttacgtttac ataccgttag gcggctctag
      421 aagaggagaa gttcgttctc agctcaacct gcttacgact ttcaccctgg gcggactgtg
      481 gcacggcgct cactatcctt atattatctg ggggtttttt cacggcctga tgctggtact
      541 cgaacgtttc ctgggactgg gtctggacaa agtcctgccc acaaagatcc aactacctcg
      601 agcactcgat atcgttcgca caggtctcca ggtcgtcttt gttttcatcc tgttctgcat
      661 tggagctgtg tttttcaact cccctactgt aacgagcgca ttcacgatgc tcggccgagc
      721 attcacgtgg gttccgggaa gtccccttca ggcaacagaa actctttcct attttgtcat
      781 cgccggcttc ctgcttaacg gcatccagct gattgagaag tggccttccc tcaaactcag
      841 tcatgcggca accatcgcag gcgtctacgg attcatcgtg atgtttctgc ttgcgaagta
      901 tgctccaggc ggtcgcgact tcctctactt tcaattctag gaaacaatat gcggcaattc
      961 aaaatccttc tggtccctct ggctctgttc gccgtagtat ttgccctgga caaagttttt
     1021 ctccttccga ttgtacgcga aaactttgta tcgtggaaaa aaatcgagcc tccattttat
     1081 gaagcaagaa gagatcttta cgatctttac aaaagatcct accaggcaga caaagcacgc
     1141 ggcaaaaaga tcggggcaat tttcggaaca tcgcgttctg ctgaattcaa cacggatggg
     1201 attgatcaaa tcctgcatga ttcgcgtacc tataacttcg gagctccatt cgcctgtccg
     1261 tcgttcttct actactggta ttctcaaatg aaagcagatg ggattttgcc agactatgtg
     1321 attcttgaaa cagatcctct gctcttcaac gaaagagcta ttgactactc tctgtcctat
     1381 tcatacgatt cgtctttcgt catcaaacat ctcgaactgg gccgtgcacg cggtggagat
     1441 ccatggtctg caaggccagg attctccttt gatgagggag aaacgttttt cttgaagaga
     1501 ctcttcgcgc tctacaggta cccgccggac ctgggagtct acaaagacaa caaccaggac
     1561 cttccgcttc ctctgccgga tggaaccatt cgaatctgga agggcaaaga atttcgcgct
     1621 gagctactca agtggatggg aagcatcaac gaattgaagt taggcggcat tcccaacatg
     1681 ctttcgatcc agttgcctcc ggaaaaactc aaaggagatg cagcaaacat ggcagccatc
     1741 cacctgactg gatgctctcc ttcgcgcact cagattgctt tttatcgcct tcttacaaaa
     1801 tccctggcag aaaagagaat tcaaacagtt gtttactggc cagtggtatc ggatgcgttc
     1861 caggaaaaaa tagacgacgc acgacttccg gattttgtgc aaagggcgcg agtggaacag
     1921 gaaatcaaaa aagtggatcc ggcaggaatc atccagttga tctatcctgc agaattgaaa
     1981 tgccgcacat tcgtcgattc agttcatcta agcggctcat gctttcctga actcgaaaaa
     2041 atactgatcg agaagggact tccggctcaa aagcggaagt aaatgaactg cactgtgctg
     2101 cggtccagtc gcaccagcat atagaacagg gcgactgcga tgacgggaat caaatagcgt
     2161 ccgtatttga gaagtggtgc ggccaatctt tcacgctggt attgcacaac gtgaacaaga
     2221 atggagagag caacgagtcc tgggaacctg gtccagttag gcggcatcac agccccggcc
     2281 gcagcatccc cgctgagagt gtgtatcatg gccatggcac ttgtgaaatc atgcccgcgg
     2341 aaaaagagag cagaagaact ccaaagagta aacatcacaa cccagagagc tgccttcgcc
     2401 gggaaagaaa attccctctt tcctattcca agcaatctct cgatagtcat gagcgcgcca
     2461 atgtagacgc cccagaaaaa gaacatgtag ctatgaccgt gccagagtcc ggccagaatc
     2521 atggtgatca aaacattcaa atgacttcgc agtggactcg tgcgagatcc accgagcggg
     2581 aaaaagatat aatcccgcaa ccacatagac agtgtgatgt gccacttgcg ccagagatcg
     2641 gcgaacgtgt aagaaaagta aggggcgcga aagttctcag gaatttcgta acccagcatc
     2701 ttggccagac cacgggctat gtctgtatat cctgaaaaat ctgcataaat ctggagtgca
     2761 aaaccaaaga gcgccgcaac cgcacccaca gtcgtgaact tcgcgggatt ggaataaaca
     2821 ggatcaatga tggaactgat tccgtctgcg atgactacct tcttcaaaag gccaaacagg
     2881 atcaggtaga ttccagccgt ggactgggtg cgatcaaatc tctccgtgcc gatctgcgga
     2941 aagtaatctc tgtgccttaa gatgggacct gcaatcagat gcgggaagaa gaagctgtat
     3001 tgtaagaaat tttgaaagcc gatctcctcg tctatgtccc cgcgccacgc atccactaga
     3061 atcgcaatca actgaaaggt gtaaaagctg atggccagag gaaaaccgaa atggagcgcc
     3121 tcgcctgcat gcgaaaatgc agccagccct gtaactccac ccagccactg caaggtggtg
     3181 gctacatact taaagaaaac aagattcgca atgttcagcg caagtccaac gtagaaccag
     3241 gacttgctgt gcttctcgcg cacgcgcaag tagcaatagt agctgatgag aactacagct
     3301 gtgaaatgaa agagataaag aaaatcccac cacgcataaa aggccatgga agaaagaatg
     3361 agccacctgc gacgccattc gccttgcagc tgccagtaga ctgcatagaa tacgaagaag
     3421 aaaactaaga aatcgaaaga attaaaaagc atccctagtc cagccggcga atctggatgc
     3481 tcgtaaaccg ttcgctgcca gcggcaacat ccgagaccac gacaatttca tctccggtca
     3541 aaacacgatt gcgagccctg agcttctcaa acgcggcacg gatattcttt tcaggatcgt
     3601 ttgagaagtc cataagaaaa ggcaccacac ctcgagcgag ccagagcttt cgcctggagc
     3661 tggacatatt cgtaaaagcg taaatgatag aggtacttgg tctgaaactc gccacctgtt
     3721 ctgcgaggat tccacgacga gtcatcacaa caatggcggg gcttttcagc gaatcggcga
     3781 gcttgcacgc gcttcttgca agctgctcgc gaaagctacc tgcaggccgg gccagtgcaa
     3841 acccaattcc tggttctttc tcaagtcttc gcgcaatt
//
LOCUS       DAORFF010000380         3869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14319_length_3869_cov_3.617724, whole genome shotgun sequence.
ACCESSION   DAORFF010000380 DAORFF010000000
VERSION     DAORFF010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14319_length_3869_cov_3.617724"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKK76_13940"
     CDS             complement(<1..300)
                     /locus_tag="PKK76_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69630.1"
                     /translation="MKQAIAIALVAFALSSVSAGEYVVFVHGRSSDHCGTGTTDVNNY
                     WGNAKNISTTLTKYFVGYDGSTDPRSSGSCRAQTSMYTVMQAVCTGANSCVIICHS"
     gene            415..1449
                     /locus_tag="PKK76_13945"
     CDS             415..1449
                     /locus_tag="PKK76_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69631.1"
                     /translation="MKFFSYDPTCAIGTDPRGCFMRLNSLVLLLFLTAACKEPAPPAS
                     AAIYAIAPATHVSTWDQNLLGRPLPARIEPATEPLLDYIRRWNESDGFQAVPKPARMD
                     AKDKEALARELSNLPPPVLKLLQKHVITIATVEDLGGSAMAGAAFDDRGEFPFGFLIL
                     DINMFKRNANQWISYKENTVFQPEPGTSYTVTLEPEVSNTRIAGMRFVILHELGHIIA
                     QVTRSLPAFTDRRPDRNKFPFARTFDEFPLRGRIKFYADKPPFSVMEAERLYSEFSPT
                     RFPTLYSCVDEEEDFSETFALYIHTVLMKKPYEVKLIEKGKTTVLARDRILLPQMKEK
                     RDFIARILEK"
     gene            complement(1498..1908)
                     /locus_tag="PKK76_13950"
     CDS             complement(1498..1908)
                     /locus_tag="PKK76_13950"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNL69632.1"
                     /translation="MNTLMRLPSLFGWADELEPVFRTELQGHTPPVEIKKGDKEYRVT
                     AHLPGVKRENLNVTVENGFLTISGKYETREEKEYQTVRSEIVSYSTFKRSLKIDQNSF
                     DVENVDAKLTDGVLEIRLPIKASVQPRQIDVKVN"
     gene            complement(2038..2922)
                     /locus_tag="PKK76_13955"
     CDS             complement(2038..2922)
                     /locus_tag="PKK76_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69633.1"
                     /translation="MIAYSVLVALLQFAILLLPLVFQTIELSELVALQFLGSIVSSWA
                     AMFLDGLKTRHLWFSLLIYVIFAAPVALLFTVSFPMMETLVSRIGLVSPTREIKSILQ
                     WGILSGVALEFAAFLLVASGLWMKSKRAPYLFRRAGTNRQEKDLLFDLRTRVVSDEMT
                     NFKYVSLQTACPGVVMEYRGDILAFLRFFLHEGTVVIFDLIILPEARVLPHSRMLPDL
                     IRKVSDQPGLENVTLAAMLPAEDEDSIQRALLSGAGFEQGDLDPELSQYIRDFRLPGF
                     EDWQPFRGPFETWLLAKD"
     gene            complement(2933..>3869)
                     /locus_tag="PKK76_13960"
     CDS             complement(2933..>3869)
                     /locus_tag="PKK76_13960"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNL69634.1"
                     /translation="GQTGEIVIKGPNVMKEYYRNPEATAKTLHNGWLRTGDLGYRDQD
                     GYYFIVGRKKEMIIRGGENIYPREIEEVLYENPEVQDCAVVGLPDKKYGEEVAAFIIL
                     KGGSSADEKTIKSYLRAKIADFKRPRIIEIVTELPRTATGKIQKNKIIEEFVGNQELI
                     SRVNGKVLIPYRWVYGKALSRFYTGLREEGKFYGTARKGCEKCNGAVQLPPKSFCGLC
                     HEECNDWVEVPNRGVVESFTTVYMEFPGQPTKPPYTYGYIKMEGTATHLYHMIREIEE
                     KDIHIGLKVEAVWAPVENRRGNLHDIQYFRPLGAG"
BASE COUNT          969 a         1014 c          924 g          962 t
ORIGIN      
        1 gctgtggcag atgatgacgc agctgtttgc accggtacat accgcttgca tcacagtgta
       61 catgctggtc tgcgcgcggc agcttccaga cgaacgtgga tccgtggatc cgtcgtagcc
      121 cacaaaatac ttggtcaggg tagtgctgat gttctttgca tttccccagt agttgttcac
      181 gtctgttgtt cctgtgccgc aatggtcgct tgagcggcca tgtacgaaca cgacgtattc
      241 acctgcagat acgctcgaaa gagcgaacgc tacaagtgct atagcaatag cttgtttcat
      301 tgatttcccc cgtgatatgg acccgattcg cttccccggc tcttcgaatc cgcccaacca
      361 gtaccggcgc agtgcaaaaa ggtcaatcca aaatattgcg cattgcacaa aaaaatgaaa
      421 tttttttctt atgacccgac ttgcgcaatc ggcacagacc cgagaggttg ctttatgcgg
      481 ctcaatagtc tggttttatt actatttctg accgctgcgt gcaaggaacc agcccctccg
      541 gcatccgcag ccatttatgc cattgcgccg gccacgcacg tgtcgacctg ggaccagaac
      601 ctgctgggaa gaccacttcc ggcgcgcatt gaaccggcca ccgaaccgct attggactac
      661 atccgcagat ggaacgaatc ggacggcttc caggctgtgc ccaaaccggc ccgaatggat
      721 gcaaaagaca aagaggccct ggcgcgagaa ctgtccaatc ttccgccccc cgtgctcaag
      781 ctattgcaaa agcacgtcat tacaatcgcc acggtagaag atttgggcgg atctgcgatg
      841 gcaggagctg cgtttgatga cagaggggaa tttccgttcg ggtttctgat cctcgacatc
      901 aatatgttca aaagaaatgc gaatcagtgg atcagttata aagaaaacac agtattccag
      961 cccgaacctg gcacgtcata cactgtgact cttgaaccgg aagtctcaaa cacacgtatc
     1021 gcaggaatgc gattcgtcat ccttcacgaa ctcgggcata tcatcgcaca ggtgacccgc
     1081 tctctgcccg cgtttaccga tcgtagaccg gacagaaata aattcccctt cgcgcggacg
     1141 ttcgacgaat tcccgcttcg cggaagaatc aagttttacg cggacaaacc acccttctcc
     1201 gtgatggaag cagaaaggct ctatagcgag ttttctccta cacgctttcc aactctgtat
     1261 tcctgtgtag atgaagaaga agatttttca gaaacatttg ctctgtacat tcacacagtc
     1321 ttaatgaaga agccgtacga agtgaagctt atcgagaaag gcaagaccac tgtgcttgcg
     1381 cgagatcgga ttttgttacc gcagatgaaa gagaagcgcg atttcatcgc acggattcta
     1441 gaaaaataaa gggcggcctt ttggccgccc cgcgaactat cgtgcagtga tctgcaatca
     1501 gttcactttc acgtcgattt gacgtggctg gacgcttgct ttgattggca gacgaatttc
     1561 gaggactccg tctgttagct tcgcatccac attttcaaca tcaaagctgt tctggtcaat
     1621 cttcagcgaa cgcttgaatg ttgagtaact tacaatctcg gaacggactg tctgatactc
     1681 tttctcttct cgagtttcgt attttccgct gatggtcaga aaaccgttct cgacagttac
     1741 attcagattc tcgcgcttta cgcctggcag atgggcagta acgcgatatt ctttgtcacc
     1801 tttcttgatc tcaaccgggg gagtatggcc ctgcaattcc gtgcggaata caggctccag
     1861 ttcatcagcc cagccaaaga gcgaagggag tctcatcagt gtattcattt gtgtattcct
     1921 cctgagccag cggctcaggc acagaattgc attgggcgtg ccagtcgcag accgcaacag
     1981 aagggcagtt ccgcgctaaa tccggagaag cgagctgaaa atttgtcagt tttgatgtca
     2041 gtctttcgcg aggagccagg tctcgaaagg accgcgaaat ggctgccagt cttcaaagcc
     2101 aggcaaacga aagtctctaa tatattgtga tagctccggg tccagatcgc cttgttcaaa
     2161 accggcgccg gataaaaggg cgcgctggat ggaatcttca tcctctgcgg gcagcatggc
     2221 tgccagagtc acgttttcga gtccgggctg atctgaaacc tttcgaatca aatcgggtag
     2281 cattctactg tgaggcaaga ccctggcctc tggcaaaatg atcagatcaa aaatcacaac
     2341 cgttccctcg tgaagaaaga atcgaagaaa ggccagtatg tcccctctgt actccatcac
     2401 cactccagga catgcagttt gcaaagagac gtatttgaag ttggtcatct catcgctgac
     2461 cactctggtg cgaagatcaa aaagcaaatc cttctcctga cgattggtac ccgcgcgacg
     2521 aaacaaataa ggtgctctct ttgatttcat ccagagcccg gatgcaacca gcaagaatgc
     2581 agcaaactca agtgcaacac cagagaggat cccccactga agtatggatt tgatttcgcg
     2641 agtgggcgaa accaggccga tgcgcgaaac aagagtctcc atcattggga aagaaacagt
     2701 gaacaacaat gcaacgggcg cggcgaaaat cacatagatg agcagagaga accatagatg
     2761 tctggtcttc aggccatcga ggaacatagc agcccagctc gatacaatcg acccgagaaa
     2821 ttgcagcgcg accagttcgc ttagctcaat cgtttggaat acgaggggca ggagtagaat
     2881 tgcaaactgc agcaatgcta caagaactga atacgcaatc aaagtctgtg actcaacctg
     2941 cacccagagg tctaaagtac tgaatgtcgt gcaaatttcc gcgacgattt tcaaccggag
     3001 cccacactgc ttctaccttc aaaccaatgt ggatgtcctt ctcttcaatt tcgcgaatca
     3061 tgtgatagag atgggtagcc gtgccttcca ttttgatgta gccgtaagtg tagggcggtt
     3121 ttgttggctg gcccggaaat tccatgtaaa ctgtagtgaa actctccacc actccgcgat
     3181 tgggcacttc tacccagtcg ttgcattctt catgacagag tccgcaaaat gatttgggcg
     3241 gcagctgtac tgcaccattg catttttcgc aacctttgcg agcggtgccg taaaactttc
     3301 cttcttcgcg aagccccgtg tagaaacgcg aaagcgcttt tccatacacc cagcgatacg
     3361 gaatcaagac cttgccattt acgcgcgata tgagttcctg gttgcctaca aactcttcaa
     3421 tgattttgtt cttttggatc tttcctgtcg cagtgcgcgg caactcagtt acgatttcga
     3481 tgatgcgtgg tcgtttgaag tctgcaattt tggcgcgtaa gtagctcttg atggttttct
     3541 cgtctgcgga agatccgccc ttcaaaataa taaaagcggc tacctcttct ccgtatttct
     3601 tgtcgggcag tccaaccact gcgcaatcct gcacctccgg attttcatag agaacttctt
     3661 cgatttcgcg cgggtaaata ttctctccgc cgcgaatgat catttctttc ttgcgcccta
     3721 caatgaaata gtaaccgtcc tgatcgcgat agccaagatc ccctgtcctg agccagccat
     3781 tgtgaagcgt cttagcagtt gcttcaggat tgcgatagta ctccttcatc acgttcgggc
     3841 ctttgattac tatttcaccc gtttgccct
//
LOCUS       DAORFF010000381         3851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14429_length_3851_cov_4.382244, whole genome shotgun sequence.
ACCESSION   DAORFF010000381 DAORFF010000000
VERSION     DAORFF010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14429_length_3851_cov_4.382244"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..727)
                     /locus_tag="PKK76_13965"
     CDS             complement(104..727)
                     /locus_tag="PKK76_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69635.1"
                     /translation="MKSHASFLRRVSVSASFALFAGCQFFTGPKITDEIVDKYVKAYT
                     GLRQIGPELAEKMKNAQGREVDIDPGMQGYAQIEKIIKDAGFKDYKEFVQVNAKIGLA
                     FSQMEGRTFMDQMDKMHSIGYEEIDKQLADPQVPEEVKAELRKTRAQLEENMAKNKPW
                     AEGVLKGASILSDKETLEVIQRNREKIRSAYTGGIPEPAGEFMGRKL"
     gene            829..1356
                     /locus_tag="PKK76_13970"
     CDS             829..1356
                     /locus_tag="PKK76_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69636.1"
                     /translation="MRFFSAAILAMAMLNCQTTAKGVPVQPSAKVKEFLGDSILGVLS
                     APDSVTAVLVEPRGGSTADHMDGFKLLSKPVALTAEQISTLQNAIMDEKTYDFLHSKR
                     HPFDPSVGFQFKKGKDTVNVLVSYYGNLWQFTGSGKTLIEDNDGARKPLLNLAKQLFP
                     KDKDIQSFKETMEGQ"
     gene            1358..2509
                     /locus_tag="PKK76_13975"
     CDS             1358..2509
                     /locus_tag="PKK76_13975"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNL69637.1"
                     /translation="MKKIVALIAFAFVCSLQAQTLHMLIVADTADASIGSSTLADLQN
                     MQNKAREIASNSGMKLNMKVLKSPEFNGTTTLAAVQAINPGPNDTVFFYYSGHGYRTR
                     ESKTKWPNMYVKKPQPGLEFSKVLEILDQKNPRLVIALGDLCNSFSDQSERSANLRAF
                     AELQPSNYKKLFVEFSGRVYASGSKPGQYSFAMEDGGAFTNQWLQALGTELRAEEPSW
                     KRLMAVATKPIRIGSPDQATQDPIFELKEGKQVVAHNTVDENAPTIQPTNTVVQQEVT
                     DAEEETEEYQEDELCPDLAELLGSLKQLDGALKPGIRVTRGDNLYNSLDTVNKALSSV
                     SEAYGDPKLQKSVTGLSTALEANNLQAYKKNLGRVIQYFETLNQDKCSE"
     gene            2497..3303
                     /locus_tag="PKK76_13980"
     CDS             2497..3303
                     /locus_tag="PKK76_13980"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL69638.1"
                     /translation="MLRIRRFLLFSFVCAASAALLFDCAGKSGPALFAAKFGESYYPS
                     HLVNTTSTREYVKLDWLFYVIEVSGKIVLVDAGMKDESVADRFRIRGFRTAPQILKLQ
                     GIFPSQVTDIVLTHAHPDHVGGITFFPNAVVHVQDAEFQAIRYAPKLAPVKKIFEEIL
                     TSGHLRVHSGDTDLYGVKILLAGGHTPGSQAAVVQTSNRAFIITGDECYFADACVAQI
                     PLPEESAFSPEKNQKFIQRVGSQNWRILTLHDPAVFSAYDARLRIVRLTQ"
BASE COUNT          936 a          973 c          954 g          988 t
ORIGIN      
        1 actactgggg gcgcgatcgc tttccgtgtg ctctttccct cgtcgagttt cgcgagacac
       61 ttcgcgttca tcacccggat cgcttccgtt ttttttacga gcgttagagt tttctaccca
      121 tgaattctcc ggcaggttcc gggattccgc cggtgtaggc ggaacggatc ttttcacgat
      181 ttcgctggat cacctccagc gtctctttgt cgctgaggat gcttgcgccc ttcaagacgc
      241 cttctgccca tggtttgttc tttgccatgt tttcttcgag ctgcgctctg gtctttcgta
      301 gctcggcttt gacttcttcg ggcacttgag gatctgccag ctgtttgtct atctcctcgt
      361 atcctatgct gtgcattttg tccatctgat ccatgaacgt tctgccttcc atttgactga
      421 aagccagccc gatcttcgca ttcacttgca cgaattcctt gtagtccttg aatcctgcat
      481 ccttgatgat cttctcgatc tgcgcatatc cctgcatccc tggatcgatg tccacttcgc
      541 gcccctgcgc attcttcatt ttttctgcca gttcagggcc gatttgcctg aggccagtat
      601 atgcttttac gtatttgtct acaatttcgt ctgtgatctt tggaccagtg aagaattggc
      661 atccagcgaa aagcgcgaaa gacgcagaga cagacacccg gcggaggaag gaggcgtgcg
      721 atttcatgtc tcagtctggc tttcaaagga aaattaagtc gaaccttttt tcttgttgag
      781 cagacgaaca tcgcgattca atctgaatca aaaatacagg agttatcaat gcgatttttt
      841 tcagcggcca ttctggccat ggcaatgcta aattgccaga ccacggcgaa gggggtgcct
      901 gtgcagccat cggcaaaagt aaaggaattt ctaggggatt cgatcctcgg cgtactgtct
      961 gctcctgatt cggttacagc tgtcctcgtt gagcccagag gaggatccac tgcggaccat
     1021 atggatggct ttaagctgct ttccaaacct gtcgcactga ccgcggagca aatctccacc
     1081 ttgcagaatg caatcatgga tgaaaaaacc tacgatttcc ttcatagtaa acgccatcct
     1141 tttgatcctt ctgttggctt ccaattcaag aagggaaaag acacagtcaa tgtgctggtg
     1201 agctactacg gaaacctctg gcaattcacc ggaagcggca agactctcat cgaagacaat
     1261 gacggagcac gcaagccact cctgaatctt gcaaagcaac tcttccccaa agataaagat
     1321 attcagagtt tcaaagaaac gatggagggt cagtagaatg aaaaagatcg ttgcactcat
     1381 agcgttcgcg tttgtctgtt cgcttcaagc ccagactctg catatgctca tcgttgcgga
     1441 cacagcggac gcgagcatcg gtagttccac tctcgcggat ctgcagaaca tgcagaacaa
     1501 agctcgcgag atcgcttcca acagcggaat gaaattgaac atgaaagttc tcaaatctcc
     1561 agagttcaat ggaaccacca cactcgcagc agttcaggcc atcaatccgg gtccgaatga
     1621 tactgtgttt ttctattact ccggacatgg atatagaacg cgcgaatcaa aaacaaaatg
     1681 gccaaacatg tatgtgaaaa agccacagcc cgggcttgag ttttcaaaag tgcttgagat
     1741 tctggatcag aagaacccac gccttgtgat cgctctcggg gatttgtgca atagcttttc
     1801 tgatcaaagc gaacgcagtg cgaatcttcg cgcattcgcg gaacttcagc cttctaacta
     1861 caagaagttg tttgtggaat tcagcggtag agtgtatgca tctggttcaa agccagggca
     1921 gtattccttt gcgatggaag acggcggtgc gttcaccaat cagtggctgc aggcgctcgg
     1981 cactgagctt cgcgcggagg aaccaagctg gaaaaggttg atggccgtgg ctacaaagcc
     2041 aattcgcatc ggttctcccg accaggcaac gcaggatccg atcttcgaat tgaaagaagg
     2101 caagcaagta gttgcgcaca acactgtcga tgaaaacgcc cctaccatac agccaaccaa
     2161 tacagtagtg caacaggaag taacggatgc ggaagaagaa acggaagagt atcaggaaga
     2221 cgaactctgt ccagatctgg cagagctgct cggttctctc aagcaactgg acggtgctct
     2281 caagccaggg attcgcgtta cccgaggaga caatctctac aacagtctgg atacggtgaa
     2341 caaagcactt agcagtgttt ccgaagcata cggagatcca aagcttcaaa agagcgtgac
     2401 cggactctcc accgcactgg aagcaaacaa tcttcaggcc tacaagaaaa accttggaag
     2461 agtcattcag tattttgaaa cgctgaatca agataagtgc tcagaataag gaggtttctc
     2521 ctattctcgt tcgtatgcgc cgcatctgcg gcgcttttgt ttgattgcgc cggcaaaagt
     2581 gggccggcgc tttttgccgc caaattcggc gaaagctatt acccgtctca tcttgtaaat
     2641 acaacgagca cacgtgaata cgtgaagctg gactggctct tctacgtcat tgaagtatcc
     2701 ggcaagatcg ttctcgtaga tgcaggcatg aaagatgaat cagtggccga tcgctttcgc
     2761 attcgcggtt ttcgaactgc gcctcaaatc ttgaaactcc agggcatttt tccttcgcag
     2821 gtcaccgaca ttgttctgac tcatgcacat ccggatcatg tgggaggaat taccttcttt
     2881 cccaatgcag tggttcatgt tcaggatgcg gaattccagg ccatccgtta tgcgcccaag
     2941 ctcgcgcctg tgaaaaagat ttttgaagaa attctaacaa gcggacattt gcgtgtacac
     3001 tccggggaca cagacttgta tggcgtgaag attctactcg ccggtggcca cactcctggg
     3061 tcgcaagcgg cggtcgtgca aacaagcaac cgggcgttca tcatcaccgg ggatgagtgc
     3121 tatttcgcag acgcttgcgt ggcacagatt ccacttccgg aagaatccgc tttttctccg
     3181 gaaaagaatc agaaatttat tcagagagta ggttcacaga attggcggat cctgacgcta
     3241 catgatccag cggtgttcag cgcatacgac gcccgtttga gaattgttcg tttgactcag
     3301 taacgaccgc gcagtttttc agaatcgttc tctgcgataa aaagaaaggc cgcccgcggg
     3361 cggcccgtac tcatttacaa gctttcctgg cagtctcgaa agcggtcttc gccgccttct
     3421 gacaatcctt ccgagcctga ccatctttcg ccttgcattc tttatgcgcg gctctatgat
     3481 cggtctttgc cttctgcaag cagtcttcct ttgggccagc aacgagagtc gttgccagag
     3541 ccatcgcaac ggctggtgca atcagtctga gtcgtttcat gattccctca tggttttaga
     3601 ttccgtagat atacggacaa tggccgcctg cggggtcaaa aaaaaaatga agagcaatgt
     3661 tgcgccaaag aaatgacgcg cacggagctt cgggtagact ggttttgtgt acgagctgcg
     3721 cgaatattgt gccgcgattt ttgctgaaca ttcaggtaag ctgttgcgtt atttgcgtcg
     3781 gcttggaggc ggcgcgaatt cggaagatat tttgcaagag gtgttcattc gcttcattcg
     3841 cgaagtgcag c
//
LOCUS       DAORFF010000382         3850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14433_length_3850_cov_3.589987, whole genome shotgun sequence.
ACCESSION   DAORFF010000382 DAORFF010000000
VERSION     DAORFF010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14433_length_3850_cov_3.589987"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PKK76_13985"
     CDS             complement(<1..217)
                     /locus_tag="PKK76_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69639.1"
                     /translation="MGGTIDLFDGLSSGLVPQGGGWIFVARFHKESNYAIFEIISYAN
                     VKRIEQSESGVEMFSRGLKAFVVYEPTN"
     gene            complement(219..1283)
                     /locus_tag="PKK76_13990"
     CDS             complement(219..1283)
                     /locus_tag="PKK76_13990"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNL69640.1"
                     /translation="MQSSKIRLGVLCGGRSSEHEISLRSAANVLRAVDRTRYDVIVVG
                     IDRAGGWQIVSENALASKELLPVGKGAAYRAESLAGALPILMTCDVIFPVLHGKMGED
                     GTMQGFLRVLDLPFIGADVAGSAVGMDKWLMKRLLVEAGLPIGRYTAFRDVQSATANF
                     ATLRERFGMPLYVKPAAQGSSVGVHRVENETDFRSAVEDALRYDDVVLVEEAIAGREV
                     ECAVLGNEEPIASLCGEIIPKGGFYSYENKYLNEDGAAFEMPANLPGHVMDRLRKLAI
                     ETYRALDCAGLARVDFFLRPDESIIINEINTLPGFTSISMYPKLFELSGLPTPQLIDR
                     LVHLARDRHVKQTAKKIDRV"
     gene            1327..1920
                     /locus_tag="PKK76_13995"
     CDS             1327..1920
                     /locus_tag="PKK76_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69641.1"
                     /translation="MKEIAFAILLLQPMQTRAPGYFLMPALREGELTAFLKSPVMDRS
                     VLAQIASARENEARAILIQRRGEIRAEICATPESERPAAIAAIRKTVEDEHALYKKEI
                     SNYEAYIYRRMEKEIDPAYREQAVAARKDAFVLQRDRSSLIEQSWMKMLSAKDVCEQT
                     ELFSEKINLPYYLALFHYQSIFPAEMRSRTLLSDTPR"
     gene            1944..3554
                     /locus_tag="PKK76_14000"
     CDS             1944..3554
                     /locus_tag="PKK76_14000"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNL69642.1"
                     /translation="MRISILLCFLFATNLAAETIDEGGASHPGFTATLYGAESFRATD
                     STQDLRLAPAPASDAILQLRFEEPSSILKDSAGNFKVRANYVAQKNGPMGGFARFIKP
                     ENRIELSSPRELWPSDGDFSVEFWIEPVIFYSRNSIFKKVSLLGGQKRGMEIYLESGR
                     LKASFFDLFEDTDGQRHSITLSSHSALPGSRWSHVTLTYDASSGKLALFVDNQEQMVS
                     YAADATGVYRASFHPLDRSAIVIGENYFGSLDEFLITGSAISPEEHPRTRLPSVHISD
                     VSFRPTQQSGMAISEVYDAGNSSSGRFRYAADEPAGSSIQFFIRFSEHAFNSDAPDAG
                     SLKWKRIRNQTDNIGRFRFFQWKALFNADPTGSVSPVLRETTLTRFALEPPARPGGLR
                     AIEELSGGDSVCLEWRNVPEPEIESEGGYIVYYGLKPGEYLGHVRIQNGKPIRPLAPE
                     KMQLTKEEKRRTQYQSSVVMRELSGRTRFMLDNRTIEENIRGKKKGNMPFLRPDRTYY
                     FAVSAYTSQGESEQSKETVAVLRPAPDL"
     gene            complement(3580..>3850)
                     /locus_tag="PKK76_14005"
     CDS             complement(3580..>3850)
                     /locus_tag="PKK76_14005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNL69643.1"
                     /translation="TESMGTSLMEAAYFGIPAIAGKTGVFEEIVRHGVTGYLFEPGNV
                     DELCRAILDIAGSDRMQLKLGRNARDIAGKEFDLARTCAAIQARI"
BASE COUNT          983 a         1077 c          924 g          866 t
ORIGIN      
        1 agttggttgg ctcgtagact acgaatgcct ttagaccacg cgaaaacatt tccacgccgg
       61 attcagattg ctctattctc ttcacatttg cgtacgaaat gatttcgaag atcgcgtaat
      121 tggactcctt gtgaaaacgg gccacaaaga tccagcctcc cccctgcgga acgaggccac
      181 tactcagtcc atcaaatagg tcaattgtgc cacccatttc aaactcgatc tatctttttg
      241 gcggtctgct tcacatgtct gtctcgcgcc agatgaacca gacgatcgat cagctgcggc
      301 gttggcaaac cagatagttc aaaaagttta ggatacatgc tgatgctggt aaacccgggc
      361 agagtattga tctcattgat gatgatcgat tcatccggcc tgagaaagaa atccacgcga
      421 gcaagacccg cacaatcgag cgctcgatat gtttcaatgg ccaattttct cagacgatcc
      481 atcacgtgac cgggaagatt ggcaggcatt tcaaaggcag cgccgtcctc attgaggtac
      541 ttgttttcgt aagaatagaa cccgcccttt ggaatgattt ctccacaaag ggaagcaatg
      601 ggttcttcat tgccaaggac cgcacattct acttcgcgcc cggcgatcgc ctcttccact
      661 aaaaccacgt catcatatcg gagcgcgtct tctaccgcag atcggaaatc tgtttcgttt
      721 tccactctgt gcacgcccac gctcgatccc tgcgccgcag gctttacata caatggcatt
      781 ccgaatcttt cacgaagagt tgcaaaattg gctgtggcag attgcacgtc tctgaaagcc
      841 gtataacgtc caatgggcag ccctgcctca acaagcagtc gtttcataag ccatttgtcc
      901 ataccaactg cggaaccggc cacatctgca ccaatgaatg gcaaatccag tacgcgaagg
      961 aagccctgca tggtgccatc ctctcccatc ttgccgtgca gaaccggaaa aatcacatcg
     1021 caggtcatga gtatgggtag agctccggcc aggctctccg cgcgatacgc cgcgcctttg
     1081 cccactggaa gaagttcttt gctggcaaga gcgttttcag atacaatctg ccacccgccc
     1141 gctcgatcaa ttccaactac aatgacgtca tacctggtgc ggtccaccgc acgcagcaca
     1201 ttggccgctg agcgaaggga gatttcatgc tccgatgacc ggccgccgca caggaccccg
     1261 aggcggattt ttgagctttg cataggtcac agtccgaaac acctggtttt accaaacaat
     1321 ttttttatga aggaaatcgc gttcgccatc ctgctcctgc aacccatgca gacccgcgcg
     1381 ccgggctatt ttctgatgcc tgcgttgcga gagggagagc tcaccgcctt tcttaagagc
     1441 ccggtcatgg accgaagcgt gctcgcccag atcgcttctg cgcgcgaaaa cgaagcccgg
     1501 gccattctga tccagagaag aggggaaatt cgcgctgaaa tctgtgcgac gccggaatca
     1561 gaaagaccgg ccgccattgc ggcgattcgt aagacagtcg aagacgaaca cgccctttac
     1621 aagaaagaaa tttcaaacta cgaagcttac atctacagac gcatggaaaa ggaaatagat
     1681 ccggcctacc gcgagcaggc ggtagcagcc aggaaagatg cattcgtttt gcaaagagac
     1741 cgatccagtc tcatagaaca atcgtggatg aagatgcttt ctgcaaagga cgtatgcgag
     1801 caaacggagc ttttttcaga gaagatcaac ctgccctact acctggcgct atttcattac
     1861 cagtccattt ttccggccga aatgcggtcc agaacgctcc tttctgacac tcccagataa
     1921 gcccgtttga ccgataatat agggtgagaa tttccatctt actctgcttt cttttcgcca
     1981 cgaacctggc cgcagaaaca atcgatgaag gtggcgcttc gcatcccgga tttacggcga
     2041 ctctctacgg ggccgaatcc tttcgagcca cagactccac tcaagatctg cgcctggcac
     2101 ctgcacccgc aagcgacgcg attctacagc tccggtttga agaaccttcc tccatcctca
     2161 aagacagcgc gggcaacttc aaagtgcgcg ccaactacgt ggcgcagaag aacggaccca
     2221 tgggaggatt cgctcgcttc attaaacctg agaacagaat cgaactttct tctcctcgcg
     2281 aactatggcc atcagatggc gatttctccg ttgagttctg gatcgagcca gtcatctttt
     2341 attccagaaa cagcatattc aagaaagtct ctctcctggg tggacagaag cgcggaatgg
     2401 aaatctatct ggagtcgggc agactgaaag cttccttctt cgatctgttt gaagacacag
     2461 acggccagag acattccatc acactatcga gtcattccgc acttcctgga tcgagatggt
     2521 cgcatgtgac tctgacgtac gacgcgagtt ccggaaagct cgcactgttt gtggacaatc
     2581 aagaacagat ggtgtcgtat gctgcagatg caactggagt ctaccgcgca tcctttcatc
     2641 ctctcgacag atccgccatc gtaattggag agaattactt tggttcgctc gatgagtttc
     2701 tcattacggg cagcgccatc tcgccagaag aacatccacg cactcgcctt ccctcagtcc
     2761 acatctctga cgtttccttt cgtccgacac aacaaagcgg tatggcgatt tcagaagtat
     2821 acgatgcagg caattcttct tccggaagat ttcgctatgc agcggatgaa cccgcgggca
     2881 gctccattca attctttatt cgattcagtg agcatgcatt caattcagat gcaccagatg
     2941 cgggttcact caaatggaaa agaatccgca accagacaga caatattggc agattcagat
     3001 tctttcagtg gaaagcattg ttcaacgcgg atccaactgg aagtgtttcg cctgttcttc
     3061 gcgaaacaac ccttacacga tttgcactcg agccccccgc aagaccgggt gggcttcgcg
     3121 ccattgaaga attgtcaggc ggagattccg tatgcctgga atggagaaac gtccctgagc
     3181 cggagatcga aagcgaaggc ggctacattg tgtactatgg cctgaaacca ggagaatacc
     3241 tggggcacgt gcgcattcag aacggcaagc cgattcgccc gctggctcct gagaaaatgc
     3301 agctaacgaa agaagaaaaa cgcagaacac agtaccagtc atccgtggtc atgcgagagc
     3361 tctccggacg aacgcgcttc atgctcgaca atcgcaccat cgaggaaaac atacgaggaa
     3421 agaagaaggg aaacatgcct ttcctgcgcc cggatagaac ctactatttt gctgtgtccg
     3481 cctacacatc gcaaggtgaa tcagagcagt caaaagaaac agtagcagtg ctgagacccg
     3541 cgccagatct atagtagaag caagcggtcc gggccgcgat taaatgcgcg cctggatcgc
     3601 cgcacaggtg cgtgccaggt caaattcttt gcccgcgata tcgcgcgcgt ttctacccag
     3661 cttcaattgc atgcggtcgg atcctgcaat gtccagaatc gcacgacaca attcgtctac
     3721 attgcctggc tcaaacaaat atccagtcac tccatgccgc acaatttctt cgaacacacc
     3781 ggtctttccc gcaatcgcgg ggattccaaa gtaggccgct tccataagcg aagtgcccat
     3841 ggactcggtg
//
LOCUS       DAORFF010000383         3832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14547_length_3832_cov_3.794281, whole genome shotgun sequence.
ACCESSION   DAORFF010000383 DAORFF010000000
VERSION     DAORFF010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14547_length_3832_cov_3.794281"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKK76_14010"
     CDS             <1..211
                     /locus_tag="PKK76_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69644.1"
                     /translation="AALGKRIDRMGISLERETEPDRQKDEIVQKAVTKVVNQLMKQGR
                     IVSTGMKYAGKKLTPAGQTPISRPL"
     gene            complement(208..2643)
                     /locus_tag="PKK76_14015"
     CDS             complement(208..2643)
                     /locus_tag="PKK76_14015"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL69645.1"
                     /translation="MLIATVHCGHPNQDSIDLSGEWKRKEANLTTVDPMVDSSSWESV
                     HVPGSVVKGLPEKFGILWVKHDFTVPAEILRNENALYMGRVAIAEEVYINGVYIGTGG
                     RWPPKGIVNTSDFFSGWNTPRLYHIPGSLLREGKNEIAVRLYYNNEGWINDAVMIGPV
                     QDLAVLHARTSFLLNDLNRILAFILIAVAVYHTLLFVRRRQDLENIFFAFLCVVWAIQ
                     SMNFALPPSLPVDYLSKERVVMGIGPFIPLSFALFFRFFFHLNSKFHKIATWALVLFF
                     VPAFFFIVFSFNLQSIRWKYNLVMPVGGVVILYITVCIVQAWRAGDRNARLISASFLI
                     IVAMTVNDLVIILSSTRGAIFLMGYAFPTLLFSIALVLANDFVRTRNQVEELNESLEA
                     KVLERTRELQTSHDKIQELKNQQDGDYFLTSLLTKPLNGNFVSGGPLSLEIFSRAKKQ
                     FHFRKWDSEIGGDLCAAHRIELNGRQYVCFLNGDAMGKSMQGAGGALVLGTVFKMIIT
                     RTQENVSSLNRYPELWLKECLAELQNVFISFDGSMLVSCMLGLLDEENGFVYYILAEH
                     PAPVIYRHSRAEFPVAVEQIRKIGTQGVEGNLRINTLQLEPGDILVVGSDGRDDLEKI
                     LPGGERAINEDETLFLRIVEKARADLPTIVEEIYKNGTVIDDLSLMRMGYLEDAAAPT
                     ENAMDVIRELKTALAKAREEKDYVRAQEIARNICERFPSDTDALFLASGLIKQAYSSK
                     PEMLAVARSFGERCKLRDPLHLRNLINLADIYRLMGDSAKAQETWKRAETLDPDHATV
                     QKLKTLLGAGRSG"
     gene            complement(2882..3400)
                     /locus_tag="PKK76_14020"
     CDS             complement(2882..3400)
                     /locus_tag="PKK76_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69646.1"
                     /translation="MLIRLSCMRAWRASVFVISFLTCSLSLDALPLRVFKVSEIVRDF
                     GTLRGQEITVEGRLVRAGYMLRSDGSILFETRTCNLMMCGKDECCNACTADVSLVEDG
                     SFGIALSGRFGGREIGCSGNECAMNCNPEIQKHFRITGLLRVEEDHGGPVRLVLEVRS
                     IGPIEKHAKGST"
     gene            complement(3449..>3832)
                     /locus_tag="PKK76_14025"
     CDS             complement(3449..>3832)
                     /locus_tag="PKK76_14025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL69647.1"
                     /translation="LFSGFFVRALGLRRLIYCGLLIFALCVPVAYFAGSLHGYWWALV
                     LLGIGWNFLFLAGSTLLSLSFSGPDRFAAQGLNDTMVFGTQAMASLAAGWLLYTVGWQ
                     SMVLIPIPFLVLVLALVIGFGRKVI"
BASE COUNT         1074 a         1003 c          879 g          876 t
ORIGIN      
        1 cgcagctctt ggaaaaagaa tcgatcgcat gggaatttcg ctcgaacgcg aaacggaacc
       61 ggatagacaa aaagacgaaa ttgtgcagaa agcagtcacc aaagtggtga accagttgat
      121 gaaacagggt agaatcgttt ctacgggaat gaaatacgct ggaaagaaac tgacgccggc
      181 gggtcagact ccgatcagtc ggccgctcta accggatcgt ccggcaccca gaagagtctt
      241 caatttctgt acggtcgcat gatcgggatc gagtgtttct gcccgcttcc acgtttcctg
      301 tgcttttgcg gaatctccca tgagcctgta aatatccgcg aggttgatca agttgcgcaa
      361 atgcaatgga tcacgcagtt tgcatctttc tccaaaagat cgcgccacag caagcatctc
      421 gggtttagag gagtaagcct gtttgatcaa gccagatgcg agaaacaaag catctgtgtc
      481 tgacggaaag cgttcgcata tattccgtgc aatctcttgc gcgcgaacgt aatccttctc
      541 ctctcttgcc ttcgcgagcg ctgtcttcaa ttcacgaatc acatccattg cattttcagt
      601 aggggcggcc gcatcttcca gatagcccat gcgcatgagg gaaagatcgt caatgactgt
      661 tccgttctta tagatttctt ccacaatggt gggcaggtcc gctctcgctt tctccacaat
      721 tcgcagaaac agagtttcat cttcgttgat ggcacgctct cctccaggaa ggattttctc
      781 gagatcatcg cggccatcag atccaaccac caggatatcc cccggctcca attgcaaagt
      841 attgatcctg agatttcctt ccactccctg cgtgccgatc ttgcgaatct gctccaccgc
      901 gacagggaat tctgcacgcg aatgacgata gatcaccgga gcgggatgct ccgccaggat
      961 atagtacaca aaaccgtttt cctcatccag aagtcccagc atgcaagata caagcatgct
     1021 tccatcaaag gaaataaaaa cattctgaag ttcagcaaga cattccttga gccacaattc
     1081 cggataacgg ttcaaagaac tcacgttctc ctgcgttctg gtgatgatca ttttgaaaac
     1141 tgtacccagg accagcgccc cacctgctcc ctgcatggat tttcccatgg catctccatt
     1201 gaggaagcaa acatattgcc taccgttcaa ttcgattcta tgcgcagcac aaaggtctcc
     1261 gccgatctca gaatcccact tgcgaaagtg aaactgcttc ttcgcacgag agaagatttc
     1321 caggctcaga ggaccgccgg acacgaagtt gccgttcaaa ggttttgtca atagcgaagt
     1381 aaggaagtag tcgccatcct gctgattttt cagttcctga attttgtcgt gactggtttg
     1441 cagttctcgc gtacgttcta gcacttttgc ctcaagcgat tcatttaatt cttctacttg
     1501 atttctcgta cgcacaaagt cattggccag aaccagagca atgctgaaga ggagtgttgg
     1561 gaaagcgtat cccatgagaa aaatcgcgcc gcgtgttgat gaaagaatga tgacaagatc
     1621 attcactgtc atagcaacaa tgatgagaaa agaagcggag atcagtcttg catttctatc
     1681 tcccgcacgc cacgcttgca cgatgcaaac agtgatgtat agaatcacaa caccacccac
     1741 aggcatcaca agattgtact tccaacgaat cgactgcaga ttgaaggaga atactataaa
     1801 gaagaaggcg gggacaaaga acaacacgag ggcccacgtc gcaatcttgt gaaatttcga
     1861 attcagatga aaaaagaacc gaaagaaaag cgcgaagctc agaggaatga aaggcccgat
     1921 ccccataacc actctctctt tgctcagata gtccacgggc aaagaaggag gaagagcaaa
     1981 gttcatactc tggatggccc agaccacgca taagaatgcg aagaagatgt tctccagatc
     2041 ctgacgcctt cgcacaaaaa gcaaggtgtg gtaaaccgca actgcaatca ggatgaatgc
     2101 gaggatgcga ttgagatcat tgagcagaaa acttgtgcga gcatgcaaga ccgccagatc
     2161 ctgcaccgga ccgatcatga ctgcgtcatt gatccatccc tcgttgttgt agtagagtct
     2221 aacggcgatt tcattttttc cttcgcgcaa aagagatcca ggaatgtgat agagcctggg
     2281 agtgttccat ccagaaaaga aatcggaagt attgactatt cccttgggcg gccaccttcc
     2341 tcccgttcca atgtagactc cgttgatgta tacttcttcc gcaatcgcca ctcggcccat
     2401 gtagagcgca ttctcattgc gaagaatctc tgccggcaca gtgaaatcat gtttcaccca
     2461 tagaattcca aatttctccg gcagcccttt gacgaccgat ccaggcacat gcacagactc
     2521 ccaggaggaa gaatccacca tggggtcgac tgttgttaga ttcgcttcct ttcttttcca
     2581 ttctccggaa agatcaatgg aatcctggtt tggatgtccg cagtgaacgg tggcaatcag
     2641 cacaaatagc gtacaagtcg cggcaaaaag aagcctgttg ctcgaagggt atttcacagg
     2701 cctgcaggat ttaagatcga gactttcagg caatcacaaa tagcgattcg cggggcttct
     2761 caaataagat tgtagcctta tttttttgcg cccccttccc cgatgaaggg aattttggcc
     2821 gcgcgaaatt ccttccaatg acgctcgccg catcgtattt cgaaaaagca taccacaggg
     2881 gtcaggtgga acctttcgca tgtttctcta tgggtccaat ggaccgcact tcaagaacca
     2941 gccgcaccgg accaccgtga tcctcttcga cacgcagtag acccgttatg cggaagtgtt
     3001 tctgtatctc tggattacaa ttcattgcgc attcatttcc ggaacatccg atctctcttc
     3061 cgccaaatct tcctgagaga gctatcccaa aggatccatc ttccaccaga gacacatccg
     3121 cagtgcatgc attgcaacat tcatcttttc cgcacatcat caaattgcat gtgcgtgttt
     3181 caaaaagaat cgatccgtct gatcgaagca tatagccggc tcgaacaagg cgcccttcta
     3241 ctgtgatctc ctgaccccga agagtaccaa agtcccgaac gatttcgctc accttgaaaa
     3301 ccctgagcgg caacgcgtct agagaaagac tgcaggtgag aaacgagatt acaaagacgg
     3361 aagctctcca ggcacgcatg caggatagac gaatgagcac gtgctagcgt tgttttttat
     3421 tgtgctctta gataaacgat cacgccctct aaatcacttt tcttccgaac ccgatgacga
     3481 gtgccagaac caggacgaga aatggaatag gaatcaggac catggattgc caaccaaccg
     3541 tatacaatag ccacccggcc gcaagactcg ccatagcttg cgtgccaaac accattgtat
     3601 catttaagcc ctgtgcggca aaacgatccg ggcctgaaaa actaagagag agtaaagtag
     3661 atcccgccag aaacagaaag ttccagccaa tccccagaag cacgagcgcc caccagtagc
     3721 catgcaaaga accggcgaag tacgcgacgg ggacgcacag agcgaagatc aagagaccgc
     3781 aatagatgag acgacgaagt cccagagctc tcacaaaaaa tccgctaaac aa
//
LOCUS       DAORFF010000384         3828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14569_length_3828_cov_3.028094, whole genome shotgun sequence.
ACCESSION   DAORFF010000384 DAORFF010000000
VERSION     DAORFF010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14569_length_3828_cov_3.028094"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="PKK76_14030"
     CDS             <1..1504
                     /locus_tag="PKK76_14030"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNL69648.1"
                     /translation="FWAATSLVIGSMIGSGIFGNTGYIQAQVGSPIMVMALWTLGGLI
                     ALAGALCYAELSTTYPHAGGEYVYLKHIFGGLPSFLTGWISLLVGFAAPAAAAALLSG
                     VYMSDFLKVVYPDTILSRFLLDETNRKILASALIILFGIFHSFGVKQSGRVQNFLTGS
                     KIIVIILFAVGGLTLAHRNPGIPIHQAFSGAVKPEGLGVGLMFVMFAYSGWNAASYLA
                     EEIKQPEKNLPRSLIAGTLFTIIIYLVMNYIYYLAVPATELAGKPDPAAQAANHLFGE
                     KTNRIFHVAFSFMLLSTLSACVMIGPRVYYAMSRDGLFFKFAGQLSHTGVPVRSIFVQ
                     CFISLLYILLGTYEDIQTYMGFSLSLFPVLTVFGLILHRKRFPKLERAYKSPLFPFLP
                     IMFILLSGLIVLTSYLDRPREGNFALSVVLAGVPMYYLWMRLRAGNSTHLLALITLQV
                     LCVLAAGVAYHVLKYNPDALHMSLAALGVEVISVAVFSYWLRRQYQSLAR"
     gene            complement(1446..2006)
                     /locus_tag="PKK76_14035"
     CDS             complement(1446..2006)
                     /locus_tag="PKK76_14035"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNL69649.1"
                     /translation="MSDRQYPARQYFSLDIPRKGPPVAAQPGILRVRRFRVSPRFDTR
                     SLVYRTGDVQYDTDFYNEYLTDAGAMVSEAARNWLFQAGSFATVVDSSSSLSEDFVLE
                     GVIQNLSGDFRSSPRAEVSVQLFLISSSATNGGVVFQKLYNRSIALPEKSPRALVVGL
                     GSGLSEIFTELETDIVSAASKRTQPH"
     gene            complement(2051..2989)
                     /locus_tag="PKK76_14040"
     CDS             complement(2051..2989)
                     /locus_tag="PKK76_14040"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNL69650.1"
                     /translation="MKSKTRYYKIGLFVMSGFLLLSVGLVLLGAGSFFKKTIRMETYF
                     DESVQGLDVGSAVKHRGVQIGTVESIGFVRNTYGELSEDDYYKYGKYVLVRIALTDPL
                     GMRGQDDTILQKMIQDGLRVRLTSQGLTGTAYLELDYLPPDQNKPLPIVWAPRSVYVP
                     SAPSTITRLSANIDRFFRKLEDSNVENLAVDLDRFLISGTKAVDDAKIPELRAEAVAL
                     LGEVRKTNNSFRELLSSPETKSIPENVGGSLAKLNQTMSRIDQLLTRNQSEMEETLEN
                     LKTASRDMKELTGTAKRYPSLLLFGTAPEKSTSFSH"
     gene            complement(2986..3744)
                     /locus_tag="PKK76_14045"
     CDS             complement(2986..3744)
                     /locus_tag="PKK76_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNL69651.1"
                     /translation="MEANLEPPFIRVSDLRVMYDGTTILENVNFEVGRGEILFIIGGS
                     GCGKSTLLKSMVGLVEPDPGSVVFGDRDLVGATGDDRSAILRRFGVMYQGGALLGSLT
                     LLENVRLPLEEYSGLPEEAQDLLALMKLKLVGLEGYDHHFPSRISGGMLKRASIARAM
                     ALDPEILFLDEPSAGLDPVTSFELDRLIYFLSRNLGITFVIISHELSSIQKIADRVIM
                     LDKSARGIIAVGKPGDLAASTVPRVKQFFSRGEA"
BASE COUNT          944 a         1019 c          893 g          972 t
ORIGIN      
        1 gttttgggcc gccacttctc ttgtgattgg aagcatgatt ggctcaggca ttttcggaaa
       61 caccggatac atccaggcac aggtaggttc tcccatcatg gtgatggcat tgtggactct
      121 cggaggactc attgccctgg cgggagcgct ctgttacgca gagttatcca ccacgtatcc
      181 gcatgctggc ggagaatacg tttacctaaa gcatatcttt ggcgggcttc cctcatttct
      241 tactggatgg atttctcttc tcgttggatt cgcagcgcct gcggcggcag cagcacttct
      301 ctccggcgtc tacatgagtg atttcttgaa agtggtttat ccggacacca tcctttctcg
      361 ctttctgctg gatgaaacca atcgcaagat ccttgcttct gcgttgatca ttctatttgg
      421 tatttttcat tccttcgggg tcaaacaaag tggccgcgtt caaaattttc tgactggatc
      481 caaaatcatt gtcatcattc tgtttgcagt cggcggcctc actcttgctc acagaaaccc
      541 gggcattccc attcatcagg cattttcagg cgcagtcaaa ccagagggac tgggcgttgg
      601 gctcatgttt gttatgttcg cctacagcgg atggaatgct gcgtcttatc tcgcagaaga
      661 aatcaaacag ccggaaaaga atctgcccag gtctctcatt gctggaactc tatttaccat
      721 catcatctac cttgtgatga actacattta ctatctggcc gttcccgcaa cagaactggc
      781 agggaaaccg gatccggccg ctcaggcagc caaccatctg tttggagaga aaacaaatcg
      841 catcttccac gttgccttta gcttcatgct cctttccact ctgtccgcct gtgttatgat
      901 cggacccaga gtgtattacg ccatgtccag agacgggctc tttttcaaat tcgcagggca
      961 gctctcgcac acgggcgttc ctgttcgctc gatcttcgtg cagtgcttta tttcgcttct
     1021 gtacattttg cttggaacct acgaagacat ccagacctac atgggcttct ccctttcatt
     1081 gtttccagtt ttgactgtgt tcggactcat tttgcacaga aaacgattcc cgaaattaga
     1141 gagagcttac aaatctcctc tcttcccatt tcttcccatc atgttcattc tactgtccgg
     1201 gctcattgtt ctaacgagtt acctggatag accgcgcgaa gggaacttcg cattgtctgt
     1261 tgtgctggcc ggagttccta tgtactattt gtggatgcgt cttcgcgccg ggaattccac
     1321 gcaccttctg gctctgatca ccttacaggt gctatgcgtt cttgctgctg gcgttgcgta
     1381 ccatgtcctc aagtacaatc cggacgcact tcatatgagt ctggcagcac ttggcgtgga
     1441 agtgatcagt gtggctgtgt tctcttactg gctgcggaga caatatcagt ctctagctcg
     1501 gtgaaaattt cagagagccc cgatccaaga cccaccacta gagcacgagg cgatttttca
     1561 ggaagcgcaa tggatctatt gtacagcttc tgaaacacca cgccaccatt tgtagcagaa
     1621 gaagaaatca agaaaagttg aacagatacc tccgcgcgag gcgaagatct aaaatcaccc
     1681 gaaagattct gtatcactcc ctctagaaca aagtcttcgc tcagagagga ggacgaatcc
     1741 acgaccgtgg caaaagatcc tgcctggaac agccagttgc gcgccgcttc cgaaaccatg
     1801 gcccctgcat ccgtcaaata ttcgttgtaa aagtctgtgt catactgcac atctccggtt
     1861 ctgtacacca ggcttcgagt atcaaagcga ggcgaaacgc gaaatcgcct gactctcagg
     1921 attccaggct gtgcggcgac cggaggacct ttcctgggta tgtcgaggct gaagtattgt
     1981 cgtgccggat actggcgatc cgacacacaa tgagaactca gcaaaagcag aaatgacaag
     2041 accaggtatt tcaatgactg aaactcgtgc ttttttccgg tgccgttcca aacaaaagaa
     2101 gggaagggta tctcttcgcc gttccggtca attctttcat atcgcgcgaa gcagtcttga
     2161 ggttttccag cgtttcttcc atttcacttt gatttctggt cagaagctga tcaatgcgag
     2221 acatagtctg attcaatttc gcaagagacc cgcctacgtt ttctggaatg ctttttgttt
     2281 caggcgaaga caatagttcg cgaaaggaat tatttgtctt acgcacttct ccaagaagtg
     2341 ccaccgcttc cgcacgcagc tccggaattt tcgcatcgtc tacggctttt gtgccgctaa
     2401 ttaaaaagcg atcgaggtcc acggcaagat tctccacatt ggaatcctca agcttacgaa
     2461 agaaccggtc tatgttcgcg gaaaggcgag tgatggtgct cggagcgctc gggacataga
     2521 cagatctggg agcccagaca atgggcagtg gtttgttttg atccggtgga agataatcca
     2581 gctcaagata tgcagttcct gtcagaccct gtgacgtgag gcgaacgcga agaccgtcct
     2641 gaatcatttt ctgaagaatc gtgtcgtcct gaccgcgcat accgagaggg tccgtaagtg
     2701 cgattcgcac aagcacgtac ttaccatact tgtagtagtc atcttcggat aactccccgt
     2761 aggtgtttcg cacaaagcca atcgattcca ccgtgccgat ctgcactcct ctgtgcttca
     2821 ccgcagaacc aacatccagg ccctggacgg attcatcgaa atacgtttcc atgcgaatgg
     2881 tctttttgaa gaaagagccg gctccaagca ataccagacc aacggacagt agcaggaatc
     2941 cggacataac aaacaaaccg atcttgtagt aacgggtctt gcttttcacg cctcacccct
     3001 tgagaaaaac tgtttcactc ggggaacggt gctcgcggca agatccccgg gctttccgac
     3061 agcgataatc cctcgcgcgg acttgtcgag catgatcacg cggtccgcga tcttctggat
     3121 gctggagagt tcatgcgata tgataacgaa cgtaatgccc agattacgag acaaaaaata
     3181 aatcaggcga tccagttcaa acgaagtaac agggtcaagt ccggctgacg gttcatccag
     3241 aaatagaatc tcaggatcca gcgccatcgc gcgcgcaatg gacgcgcgct tcaacatacc
     3301 tccactgatg cgcgaaggga agtgatgatc ataaccttcg agacccacaa gcttgagttt
     3361 catgagtgcg agcaaatcct gagcttcctc gggtaagccc gaatactctt ccaggggaag
     3421 tcttacattt tcaagcaatg taagagagcc cagcaatgct cctccctgat acatcactcc
     3481 gaatctgcga aggatggcag atcgatcatc acctgtagca cctacaagat cgcgatcacc
     3541 aaacacaaca gaacctggat ctggttctac aagaccaacc atgcttttga gcagagtact
     3601 tttgccgcaa ccgctcccac caatgataaa cagaatttcc ccgcgcccga cttcaaaatt
     3661 cacattttcc agaatcgtcg tgccatcgta cataacgcga aggtccgaaa cgcgaatgaa
     3721 aggtggttcc aggttcgctt ccatcaccat cccataaaat agaagatcac ggcaaagatc
     3781 ccgtcaaaaa ctgcgatcaa aatgattccg ctgactacag cactggtc
//
LOCUS       DAORFF010000385         3818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14620_length_3818_cov_3.462131, whole genome shotgun sequence.
ACCESSION   DAORFF010000385 DAORFF010000000
VERSION     DAORFF010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14620_length_3818_cov_3.462131"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..619)
                     /locus_tag="PKK76_14050"
     CDS             complement(119..619)
                     /locus_tag="PKK76_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69652.1"
                     /translation="MLEKLNPRERLLIVAFGGTLLLLLLGFIGLKVKEQRDTIRQSVG
                     EARDDVTRMSRLVADIKGMPAGGPLPDENEFLAKTTQLLESMKFTPQNIRSRKENPTR
                     TEEQIIVEMTFAGVSLKDAINFLHAVEYGKQIPARVGVFEFRKPLAQREIYDMRITLV
                     ITRPKK"
     gene            complement(619..2268)
                     /gene="pilM"
                     /locus_tag="PKK76_14055"
     CDS             complement(619..2268)
                     /gene="pilM"
                     /locus_tag="PKK76_14055"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNL69653.1"
                     /translation="MSILARKELVVDYGSTHIKGALVESSPLGGRRILRLETLPIVSL
                     RTDGDNQAEEYEYNLVRFVQSFFPEEESFLLNLPMDRVYVRDIRVPVVTAKQIDEVMP
                     FEVEPLLPVSLENAEVIGQPWAIGEESSNVITFTAQHKDLQRTVLPILRGTASVQMLS
                     VDCVGLAGASTLIPAEVSQDKMVGQIDIGGSYTIVNFVKNGTLVFTRRIAVGGVHITD
                     VVAETLGLDISTAEDKKIELDIDLAEDTEKTERSESYYRRHRIDKKTLTKLHKKIREV
                     LDDLVQEVERSVLSLPAQEDPVVWYLSGGGSLMRGTAEFLSTRLDSPVRNYPVQLSPS
                     QKEISRFMTAIGTLEHHKEKQASRFDFLATPFGTLLRRRDFTLKAMSTPILFASIGLI
                     VLLASFLSGIVLDRRQIRQYRDQLELIARDLPPDVKSAGDYLEAAEKLCKERLQYRKN
                     QTGSARVLDVLRELTNRTPGPTEAPFSLKSFRFQEGKVDFEAEVGNFGDVSKVKDALA
                     ASNSFTRIDVQPGNLMPNQKVRIAVRLQVKEQAGGSSGGCR"
     gene            complement(2269..3408)
                     /locus_tag="PKK76_14060"
     CDS             complement(2269..3408)
                     /locus_tag="PKK76_14060"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNL69654.1"
                     /translation="MINWLLKIPGIKSRRGAMAILFTFALGAAANTIAAKFFSDAQIQ
                     FASVKVAADGFRARQLALAGFQAGLAALRAAPEEYLYTSGLALKPPDISVSADCKPGC
                     FVSYRLLPEDGRLNLNNLVHSFDDKPNEQYRAVFKRFFPQYDISQENVDSLIDWIDED
                     DSPTGNGGENSYYASLKPPRKCKNFRMFSLGEITLIKGFTSDMIFASRAPANWEKTQK
                     ELSFQTEDEKNLITMDDWVAANDLTAYVPLGDRMEDKINVNAARYHTLMALSESMSRE
                     AVLALFKYRRQKGGYIKELGDLRNLPEFQRKTPADITLFDELAGNAGKLAGLLKTEGE
                     IYRIVGVGTITSGSSSSDNAKGAVIRRVTALYDKNNNKVIYYSED"
     gene            complement(3410..>3818)
                     /locus_tag="PKK76_14065"
     CDS             complement(3410..>3818)
                     /locus_tag="PKK76_14065"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HNL69655.1"
                     /translation="VFAARPDPDTRSDRITFSAVHPVSGTGGSIVREVSFYLKEMPVQ
                     PDQGEKRYILMKREEEPVDKKPGEGGLHYPLLENVTGLHFKYSLNGREWMDKWNTKQS
                     RRIPRLIQIQLTVRIGGKARTLETLASPGLYLN"
BASE COUNT          866 a         1001 c          907 g         1044 t
ORIGIN      
        1 ctatgctcct cttcgtcgaa gtcaggttct ccagcggctt cttcaggctg aatctccgcc
       61 gcaacctgct cttcttcttc ctctgtatcg agcgaattct cttcttctgt gtccgcgatc
      121 atttctttgg cctcgttatc acgagagtga tgcgcatatc atagatttct ctttgcgcca
      181 gtggctttct aaactcaaat acgcctaccc ttgccggaat ctgctttccg tattcaactg
      241 catgaagaaa attgatggcg tctttcaagg aaacaccggc gaacgtcatc tctacaatga
      301 tctgttcttc tgttcttgta ggattttcct tgcggcttcg gatattctgc ggagtaaatt
      361 tcatgctctc gagcaattga gttgtctttg cgaggaattc gttttcatcc gggagaggtc
      421 cgcccgcagg catgcccttg atatccgcta caaggcgact cattctggtc acgtcatcgc
      481 gagcttcgcc cactgactgc cggatcgtat ctctctgttc ctttactttc aatccaatga
      541 agcccaacaa caggagaaga agggttccac cgaatgcaac aatgaggagt ctttcgcgag
      601 gattgagttt ttcaagcatc agcgacaccc tccactcgaa ccaccggcct gctccttgac
      661 ttgaagcctg actgcgatcc tgactttctg gttgggcatg agattgcctg gttgtacatc
      721 gattcgggtg aatgaattgc ttgcggccag cgcatccttt acttttgaaa cgtccccgaa
      781 gttgcctact tccgcttcaa aatccacttt gccttcctgg aaacgaaaac tcttcaaaga
      841 aaagggagct tccgtggggc caggtgtgcg attcgtaagt tctcgcaata catcgagcac
      901 gcgagcgctt ccggtctggt tttttctata ctgaagacgt tctttgcaga gtttctctgc
      961 tgcctccaga taatcgccgg cgctcttaac gtcaggcggc aaatcgcgcg caatcagttc
     1021 aagctgatct ctgtactgac gaatctgcct gcgatccaga acaatacctg aaagaaaaga
     1081 agcaagcagt acgatgagcc caatcgatgc gaaaaggatg ggtgtggaca ttgctttgag
     1141 cgtgaagtct cttcttctaa gcaatgtccc aaaaggagtc gcgaggaaat caaatcgact
     1201 ggcctgcttt tccttgtgat gctccagcgt tccaatcgca gtcatgaacc tggaaatttc
     1261 cttctgcgaa ggcgaaagct gaaccggata atttcgaaca ggcgaatcca gcctggtgga
     1321 taggaactcg gcagtccctc gcatcaaaga tccaccgccc gataaatacc agactaccgg
     1381 atcttcctgg gccggcaggg aaaggacgga tcgctccacc tcttgcacca gatcgtccag
     1441 gacttcgcgg atttttttgt gaagcttcgt cagcgtcttt ttatctatcc tgtgtcttct
     1501 gtaataggac tcgcttcttt ctgttttttc tgtgtcttct gccagatcaa tatccagttc
     1561 aatctttttg tcttccgcag tggaaatatc cagacccagt gtttcagcga ccacatccgt
     1621 tatgtgaact ccacccaccg caattctgcg tgtgaagaca agagtcccat tctttacaaa
     1681 gtttacgatt gtatagctgc cgcctatgtc gatctgcccc accattttgt cctgcgacac
     1741 ttccgccggg ataagggtgg aagcaccggc caggcccacg cagtctacag agagcatttg
     1801 caccgaggct gtcccgcgaa ggataggcag aaccgtgcgt tgcaagtcct tgtgttgcgc
     1861 agtgaaagta ataacgtttg aactttcttc gcctatcgcc cacggttgcc cgatcacttc
     1921 ggcgttttcg agcgaaacgg ggagaagagg ttctacctca aaaggcatga cttcatctat
     1981 ttgtttggct gttaccactg gaacgcgaat gtcgcgcaca tagacgcggt ccatgggcaa
     2041 gttcaacagg aaagattctt cttctggaaa aaaagactga acgaaacgaa cgaggttgta
     2101 ttcgtactct tctgcctgat tgtctccgtc cgttctcaag gacacaatgg gcagagtctc
     2161 aaggcgcaga attctccgtc ccccgagagg gctcgattcg accagggcac ccttgatatg
     2221 ggtgctccca tagtccacga cgagttcttt tcgcgccaga atcgacattc agtcttccga
     2281 gtagtagatc accttgttgt tgttcttatc gtagagagcg gtcacacggc gtatgactgc
     2341 ccctttcgca ttatcggaac tcgatgagcc ggaagtgatt gtcccgaccc caacaattcg
     2401 gtagatttca ccttcggttt tcagcaatcc tgccagtttt ccagcgtttc cggccaattc
     2461 atcaaataga gtgatgtctg cgggagtctt tctctggaat tcgggcaggt ttcgcaaatc
     2521 gcccagttcc ttgatgtatc cgcctttctg tcgtctgtac ttgaagagag caaggaccgc
     2581 ttcccggctc atcgattcag aaagagccat cagagtgtga tacctcgcgg cattcacatt
     2641 gattttatct tccatacgat cgcccagggg gacataagca gtgaggtcat tggccgcgac
     2701 ccagtcatcc attgtgatga ggttcttttc gtcttctgtc tggaaggaga gttccttttg
     2761 cgtcttctcc cagttagccg gtgcgcggga tgcgaagatc atgtcagaag tgaacccttt
     2821 gatgagagtg atttctccca gagagaacat tctaaagttt ttacactttc gcggcggctt
     2881 tagagaagcg tagtacgaat tttcgccgcc atttcccgta ggagagtcgt cttcgtctat
     2941 ccagtcgatg agtgaatcca cgttctcctg cgagatatcg tactgaggaa aaaatctttt
     3001 gaataccgcg cggtactgtt cgtttggctt gtcgtcaaag ctgtgcacaa ggttgtttag
     3061 attgagcctt ccgtcttcag gcaggagcct gtaggaaaca aaacaacccg gcttgcagtc
     3121 tgccgagaca gaaatatccg gtggcttaag agccagtcct gatgtatata gatattcctc
     3181 tggagccgcg cgcaaagcgg cgaggccggc ctggaatcca gccagagcaa gctgccttgc
     3241 gcgaaagcca tctgctgcca ccttcacaga tgcgaattga atttgagcgt ccgagaaaaa
     3301 tttcgcagcg attgtgtttg cagccgcgcc cagggcaaac gtaaatagaa tcgccattgc
     3361 acctctgcga ctcttgattc cagggatttt aagcagccaa ttgatcatat tagtttagat
     3421 ataaacccgg gctggccaga gtttcgagag ttcttgcttt tccaccaatg cgaactgtga
     3481 gctggatttg aatcaacctc ggaatgcgcc gggattgctt tgtattccac ttatccatcc
     3541 actctcttcc gttcaatgaa tacttaaagt gcagtcccgt tacattctca agcagtggat
     3601 agtgcagacc gccttctcct ggtttcttgt ctacaggctc ttcttctctt ttcatgagga
     3661 tgtatctctt ttccccttga tccggctgta ctggcatttc cttgagatag aaggaaacct
     3721 ctcttacaat cgaaccaccc gtgccggaaa ccggatgaac cgcagagaac gtgatgcgat
     3781 ccgatctggt atccggatcc ggtctggcag caaaaaca
//
LOCUS       DAORFF010000386         3818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14622_length_3818_cov_3.330853, whole genome shotgun sequence.
ACCESSION   DAORFF010000386 DAORFF010000000
VERSION     DAORFF010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14622_length_3818_cov_3.330853"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..487
                     /locus_tag="PKK76_14070"
                     /pseudo
     CDS             1..487
                     /locus_tag="PKK76_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
     gene            640..1341
                     /locus_tag="PKK76_14075"
     CDS             640..1341
                     /locus_tag="PKK76_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69656.1"
                     /translation="MIPPFQDHPLVEEFNELKKYFRQHETEASRRRIKQFRSFARVIA
                     EAGLPVGFDVMGSVNFGLATENSDVDLVLYVVCEDGYEGECPSPHCRTQQYVENLLIH
                     TWIRGISDSPYPVQVVDCINLNLLKRDLEKGDPESSCLLRFAFYRSVCRGVNARLLRP
                     YHKALLENQELVEKMKPYLWTLFDELVKSSKHNYSLMKYQDRLSQLGIGIPQSILGRI
                     RGHLDQSRRHHGDGI"
     gene            complement(1348..2793)
                     /gene="hslU"
                     /locus_tag="PKK76_14080"
     CDS             complement(1348..2793)
                     /gene="hslU"
                     /locus_tag="PKK76_14080"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNL69657.1"
                     /translation="MNDLDHLTPAQIVSELDKYIVGQNAAKKAVAIAIRNRIRRRKLP
                     PEIQEEIYPKNILMIGPTGCGKTEIARRMSRLLNAPFLKVEATKYTEVGYVGRDVESM
                     VRDLLNTAISLVKKEYREKVEVDAKNAAEERLLDLLLPSSSHHKEPAAGSNFSDLVAN
                     ISNIGARIQTGMNVEEKQKDPEEESRHARARELMKQKLADGSMEDREVEFDVQAQASP
                     MVQVLSGHNMEEIDMQVQNMLGDFLPKKNRKRKLPISEARKILLNEEIERLVDQDRVI
                     VEAIRRTEQTGIIFIDEMDKICGKSAGSGPDVSREGVQRDLLPIVEGATITTRHGPVK
                     TDHVLFIAAGAFHQSRPSDLIPEIQGRFPIRVELEKLTEKDFRQILVGPRNSLTEQAR
                     QLLATEQVEVVFMEDGLDELAQFAHRVNEETENIGARRLHTILDRLLEDLSFRAPDLA
                     ADQRRVVIDRAFVLERLKPVMEDRDLSRYIL"
     gene            complement(2790..3323)
                     /gene="hslV"
                     /locus_tag="PKK76_14085"
     CDS             complement(2790..3323)
                     /gene="hslV"
                     /locus_tag="PKK76_14085"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNL69658.1"
                     /translation="MSDFHSTTILCVRRDNRTVIGGDGQVSLGPTIMKSGARKIRKLA
                     GREILSGFAGASADAFTLFELFEKKVEQYQNNVGRAAVELAREWRTDRMLRRLEAMLV
                     VADNKEMYLISGTGDVIAPDDGVLAVGSGGNYALASARALVANTQMSAREIVEKSLQI
                     AADICIYTNHNIAIEEL"
     gene            complement(3369..>3818)
                     /locus_tag="PKK76_14090"
     CDS             complement(3369..>3818)
                     /locus_tag="PKK76_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNL69659.1"
                     /translation="SEALSLNVMNESEDLTQLKILGKGRRERIIFLGDSARAALVHYL
                     KVRDQFRPATQKLFVNRDGGPLSDRGVRKIMVELQRASGLQRRVHPHRFRHSMATDLL
                     NEGADIRAVQELLGHRSLASTQVYTGVSKDRLKEVYRRSHPHGRSLD"
BASE COUNT          917 a         1006 c          906 g          989 t
ORIGIN      
        1 cattgacctg cgctacactg cactgagtgc cagaagcatc catcatgcca cgcgtgaatt
       61 tgatcgcctt acgattgcag ggtatggcgg agctcccgtg gccacatctg gaataccgga
      121 aaaactctct gttgtatttc acgaacgaat catagacgga gtactctatt ctgaaccgga
      181 gaatttcttt gatgacattg tcccggatat ctgtgtgatt cacaacccgg cctacggttt
      241 ttttgaccgc atcccatcca tggggcacgt aggatctcag ggactgcgca attacctgga
      301 cgatcattcc ccgcttcttg ccgtttccgg gcatgtgcac gaagattacg gcatagcgaa
      361 aaagaacgga acgatactcc tgaatccgtc taactttggc ggagtggatt ccattgaagg
      421 ataccagcca ggtggaacgt acgcggagat atacattgaa aacaagaacg tgcatcgggt
      481 gcgtttgatg attctaaagg gagaagaaag cattccgctg atggacatca atgtggatga
      541 aaatggactc cacgcagtga tgatgccgca ttccaaagag ctttctcacc tggacctcac
      601 tgcattcgtt cgtgacgcaa gcgggaggcc aatcgcacaa tgattcctcc gtttcaggat
      661 caccctctgg tagaagagtt caacgaactc aagaagtatt tcaggcagca cgaaacagaa
      721 gcttctcgtc gcagaatcaa acagtttcgc tccttcgcac gcgtgattgc agaggcaggt
      781 cttcccgtag gttttgacgt catgggaagc gtgaactttg gtctggcaac agagaactca
      841 gacgtggatc tggttctcta cgtagtctgc gaagatggat acgaaggcga atgcccctct
      901 cctcactgcc gcactcagca atatgtggaa aatttactga tccacacgtg gattcgcgga
      961 atcagcgatt cgccttaccc ggtgcaggta gtggactgta ttaatctgaa tttactgaag
     1021 agagatctgg agaaaggaga tcctgaatcc agctgccttt tgagattcgc attctatcgc
     1081 agtgtatgcc gcggagtgaa cgccagattg ctcagaccat accacaaagc ccttctcgaa
     1141 aaccaggagc tggtagagaa gatgaagcca tatttgtgga cgttattcga cgagctggtc
     1201 aaaagctcga aacacaacta ttcgcttatg aaatatcagg atagactctc tcagctcggg
     1261 atcggaattc cacaatccat actcggaaga atccgcgggc acctggacca gagcagacgc
     1321 catcatggag atggcattta gccctgttta aaggatgtag cgagaaagat cacgatcttc
     1381 catcacaggc tttagcctct caagaacaaa agcacgatca atcacaactc tcctctgatc
     1441 cgccgccaga tccggggcgc gaaaggataa atcctcaaga agacgatcca gaatcgtgtg
     1501 caggcgacgc gcgccaatgt tttctgtttc ttcgttgact cgatgcgcaa actgagccag
     1561 ctcatcgagg ccatcttcca taaatactac ttccacctgt tctgttgcca gaagctggcg
     1621 ggcctgctct gtgagcgaat tgcgcggacc aaccaggatt tgcctgaaat ccttttcagt
     1681 gagtttttcc agctccactc gaattggaaa tctaccctga atttcaggaa ttaagtcaga
     1741 gggcctggat tgatggaatg cacccgcagc aatgaacaaa acgtgatccg tctttacggg
     1801 gccgtgtctg gtagtgatgg tggctccctc tacaatcgga agcaaatctc tctgcacacc
     1861 ttcgcgcgaa acgtcaggac cacttcccgc tgattttccg cagatcttgt ccatttcatc
     1921 gatgaaaatg attccagtct gttctgtcct gcgaatggct tccacgatca cgcggtcctg
     1981 atccacaagt ctttcaattt cttcgttgag tagaatcttg cgcgcttctg aaatgggtag
     2041 ctttcgcttg cgatttttct ttggaagaaa atctcccagc atgttttgca cctgcatgtc
     2101 tatctcttcc atgttgtggc cggacagaac ctggaccatg ggagatgcct gagcctgcac
     2161 atcaaattcc acttctctgt cttccataga accatctgcc agtttctgct tcatcagttc
     2221 gcgagcacgg gcgtgcctgg attcttcttc tggatctttc tgtttttctt ccacgttcat
     2281 tccagtttga atgcgcgcac caatgttaga gatattggcc accaggtccg aaaaattaga
     2341 tcctgcggcg ggttccttat ggtgagagga agatggaagt agaagatcga gcagtctttc
     2401 ctctgctgcg ttctttgcgt ccacctccac cttctcgcgg tattccttct ttaccagaga
     2461 gatagcagtg ttgagcaaat cgcgcaccat ggactcaacg tcccgaccca cgtagcccac
     2521 ttccgtgtac ttggttgctt ccacttttaa aaagggagca ttgagcaggc gtgacattct
     2581 tcgcgcaatt tcagtttttc cgcatccggt aggaccgatc atcagaatgt ttttgggata
     2641 gatctcttcc tgaatctccg gaggcagttt gcgtcttctg attctattgc gaatcgcaat
     2701 ggccacggcc ttcttcgctg cgttttgccc gacgatgtac ttatcgagtt cgcttacgat
     2761 ctgcgcggga gtcaaatgat cgagatcgtt catagttcct caattgcaat gttgtgattt
     2821 gtataaatgc agatatccgc cgcaatctgt aacgatttct ccacgatttc tctggcagac
     2881 atttgagtat tcgcaactag agcacgcgcg gaagcaagag catagttccc accggagccc
     2941 actgcgagca ctccgtcatc tggagcaatg acgtcccctg ttccgctgat cagatacatt
     3001 tctttgttgt ccgcgacgac gagcattgcc tcgagtcttc gcagcattct gtccgtgcgc
     3061 cattcccgcg ccagttccac agcagcgcga cccacattgt tctgatactg ttctactttt
     3121 ttctcaaaca attcaaagag agtaaaagca tccgcagacg caccggcgaa tccagaaagt
     3181 atctctctgc cggcgagttt tctaattttg cgcgctccac ttttcatgat cgtggggcca
     3241 agactgacct gaccatctcc gccaatcaca gtgcgattgt cgcggcgcac acatagaatt
     3301 gtagtcgaat gaaaatcgga catagtcctt tttcacagag tctctccggc gggcaaaccg
     3361 ttttaggttc agtccagaga ccggccgtgt ggatgagacc gtcgatacac ttctttgaga
     3421 cgatcctttg aaactcctgt atatacctga gtgcttgcca ggctgcggtg tccaagcaac
     3481 tcctgcactg cgcgaatgtc cgcgccttcg ttgagcaaat cagtagccat gctatgacga
     3541 aaccgatgag gatgaactcg cctctgcagt cctgaagctc tctgcaattc taccatgatc
     3601 tttcgcacgc cgcgatcgct cagtgggccg ccgtcgcgat tcacaaacaa cttctgcgtt
     3661 gccggacgaa actgatcgcg cactttgagg taatgcacga gagcagccct ggcagagtcg
     3721 cccagaaaaa taattctctc tcttctaccc tttcccagaa tcttaagctg agtgagatct
     3781 tccgattcat tcatcacgtt cagagagagt gcttcgga
//
LOCUS       DAORFF010000387         3812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14669_length_3812_cov_3.226511, whole genome shotgun sequence.
ACCESSION   DAORFF010000387 DAORFF010000000
VERSION     DAORFF010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14669_length_3812_cov_3.226511"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1140)
                     /locus_tag="PKK76_14095"
     CDS             complement(85..1140)
                     /locus_tag="PKK76_14095"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNL69660.1"
                     /translation="MYHRRFKNRPGRKILNLQGRFSLIYTGGGNFVYTLPGKDTPVVR
                     RLDLSSWKYRVSLSAGAFALFLFSGFGIWTSQGTASSSGVVVEEARISPAELEKRSEA
                     LKEKIIKNNAPHVDQSFEYTVRPGDTLSEIAAVYKVLPASILSASGVKRADEVKPGQK
                     LTIPNRPGLMYRMKPGDTLAAVAQFYTVSLEDIQNDNPELRDMDLLRPGTKVFLPNAK
                     IPPPPPIWFRPAWGNLSSGFGWRPDPFSPGRMNMHTGFDIVMYYRPVHAARTGVVVYA
                     GGMGSYGMAVVIQHGDGLKTLYAHLSSVRVRSGQDVQAGQEIGISGNTGLSTGPHLHF
                     EVIQNGKPVNPARFVRF"
     gene            complement(1221..1619)
                     /locus_tag="PKK76_14100"
     CDS             complement(1221..1619)
                     /locus_tag="PKK76_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNL69661.1"
                     /translation="MAGRNDSMNSVIGPGSIFEGKFYISGSLKIDGKFEGEIKTEDAL
                     VVGETGKIKTNITAKNVVVAGTMIGNIQAESEVRLEKSGKLLGDITAPMLNIQPGVIA
                     KGTITITGGQKKDVRKVIEESYGGLKISEK"
     gene            complement(1665..3521)
                     /locus_tag="PKK76_14105"
     CDS             complement(1665..3521)
                     /locus_tag="PKK76_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69662.1"
                     /translation="MGRLILLLLLPGALFSQGLYKQPSEIIDPAQVDDLEKSALGLNP
                     DCEAILTRANNLLSSYYSQFIADKRIQDERRNPSFAAETLSENTVRLIALARSKEPGR
                     EYFLGAQPYLYRLHRLKGVCYHKTEEHTKALNEYAMAFRYTALDPAYQDNPDKNELSA
                     IYLRMAQNFGDKERARQEQDPALRSDSTGLRDLVDRFIQTSGALEAAEKQISVEEARV
                     VRGKGGNPAAARAKRDSLKQQVDSLRSQVEGVRKGSYRTFALRCQKENGELAFTMANI
                     VQELEQKNQEVERILNRSSFYRGIGNELGEDRTSYSGFTGYGILLEFANKIDPSNLLY
                     ISKLADEHRKNRRRDLAIRFYEDYFAKASALAQKPADFADNLLKLAGVFTDSQNYIRA
                     ADMLEQYDALVSSPAAKLQLADIHYYRTGRTARAKELYDAYMAARPATAGTLKDRVES
                     GLVAFRVQKNLASIARRNLRTGEEMGALAAAENVFRSLEKEEADARKVEEDLRAKLFE
                     IKRRLLGREDEDLQREYYRLQRIELAQAMEQTGYVHTRVDSMNIGQVLERQAYLAERQ
                     RNFDLAREKYRELVVRGTGPEQSRARENLERLRLSLADGLLRPPSLSPDFER"
     gene            complement(3540..>3812)
                     /locus_tag="PKK76_14110"
     CDS             complement(3540..>3812)
                     /locus_tag="PKK76_14110"
                     /inference="COORDINATES: protein motif:HMM:NF016156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNL69663.1"
                     /translation="IFETAARYQMYHAIAMLALGLAMDRVGKSGRAVLGLWLFGTIVF
                     SGSLYAIVLTGVRAFGAVAPIGGLSLMAGWAFLFVSAVRHEGFPPR"
BASE COUNT          839 a         1001 c          968 g         1004 t
ORIGIN      
        1 tagggttgtg gtgatgtggc caggccggaa ggcccggaaa cggggggatg gttctcgttt
       61 tcctctcagg tgtttggtgt caaatcaaaa tctaacaaat cgcgccggat ttaccggctt
      121 gccgttttga atgacttcaa agtgtagatg tggtcctgtc gaaagtccag tgttacctga
      181 aattccaatc tcctgccctg cctgtacgtc ctggccgctt cgcacacgca cactgctgag
      241 gtgagcgtat aacgtcttga gtccatctcc gtgctggatg acaacagcca ttccgtaaga
      301 ccccattcct cccgcataca caacgactcc agtgcgcgct gcatgtaccg gtctgtaata
      361 catcacaata tcaaatccag tgtgcatatt catgcgccct ggactgaacg gatccggtct
      421 ccatccgaaa cctgaagaca ggttgcccca tgctggacga aaccagatgg ggggcggtgg
      481 tggaatcttt gcattgggaa ggaatacttt tgtgcccggg cgaagcaggt ccatatcgcg
      541 cagctcggga ttatcgtttt gaatgtcttc tagactcaca gtgtaaaatt gcgcgactgc
      601 agccagagtg tctcctggct tcatgcgata catgagacca ggacgattgg gaatggtgag
      661 cttctggcct ggctttactt catccgcgcg cttcacaccg gaagcagata ggatacttgc
      721 tggaagaact ttgtatacag ctgcaatttc ggataacgta tctccgggac gaaccgtata
      781 ctcgaaagac tgatctacat gaggagcgtt gttcttgatg atcttttctt tgagcgcttc
      841 tgatctcttc tcgagttcag caggagaaat gcgcgcttct tctaccacca ccccactgga
      901 actcgcggtt ccctggctgg tccagattcc aaagcctgaa aaaagaaata atgcaaatgc
      961 acccgcgctc aaactgacgc ggtacttcca cgaactcaga tcgagcctgc gaacgactgg
     1021 agtgtctttg cctgggagag tgtagacgaa gtttcccccg ccagtataga tgagcgaaaa
     1081 tctaccctga agattcagga tttttcgacc cgggcggttt ttaaatcgtc tatgatacat
     1141 ggagtcgaag gggccttttt aaccaatcgg cgagagggag gaagtttgcg agcaaaaaat
     1201 tatgtcacat tagccacctc ttacttttcg gagattttta accctccata agattcttca
     1261 atgaccttac gaacgtcttt cttctgccca cctgtgatgg tgatggttcc ctttgcgatg
     1321 acgcctggct ggatattgag catgggggcg gtgatgtccc cgagcagctt gcctgatttc
     1381 tcaagtctta cttcgctttc tgcctggatg ttgccgatca tcgtgccggc caccaccacg
     1441 tttttcgctg tgatgttggt cttgatcttg ccggtttccc cgactacgag tgcgtcttct
     1501 gttttgattt cgccctcgaa cttcccatca atcttcagag atccggaaat gtagaacttt
     1561 ccctcaaaga tcgacccggg accgatcacg ctgttcatgg aatcgttgcg gcctgccatc
     1621 gagtcagttg tcccttttgc cggttttctt acaactaaaa aagatcatcg ttcaaagtcc
     1681 ggagacaagg aaggcggtct gaggaggccg tctgcgagac tcaagcgcag tctttccagg
     1741 ttttcgcgag cgcgagattg ttcgggaccg gtcccgcgca caaccagctc tctgtatttc
     1801 tcccgggcca gatcaaagtt gcgctgtctt tctgcgaggt atgcctgcct ctctagtacc
     1861 tgaccaatgt tcatggaatc gactcgagtg tgcacgtatc cagtctgttc cattgcctga
     1921 gccagttcga tcctctgcag acgatagtat tccctctgga ggtcttcgtc ttcccttcca
     1981 agaagccttc gcttgatttc aaaaagcttc gcgcgcaaat cttcttctac tttacgcgca
     2041 tccgcctctt ctttctccag agatcgaaac acattctcag cagcggcaag tgcccccatc
     2101 tcttcgcctg ttctgagatt tcttcgagca atcgacgcga ggttcttttg tacgcgaaat
     2161 gcgacgagtc ctgactccac tctatctttc agggtgcctg cagttgctgg gcgtgcagcc
     2221 atgtaggcgt cgtaaagttc tttggcacgc gcagttcgac ctgtgcgata gtagtgaatg
     2281 tctgccagct ggagtttggc tgcggggctc gatacaagtg catcatattg ctccagcatg
     2341 tcagcggcgc gaatgtaatt ctgtgaatca gtgaatacac cggcgagctt caagagattg
     2401 tctgcaaagt ctgcaggctt ttgagcgagc gcggacgctt ttgcaaagta gtcttcatag
     2461 aaacgaatgg caagatcacg tcttcgattc ttcctgtgtt cgtctgcgag tttagaaatg
     2521 taaaggaggt tggaaggatc gatcttgttt gcaaactcga gtagaattcc atacccggta
     2581 aaaccggaat aggacgttct atcctctccc agttcgtttc caattccgcg atagaaactc
     2641 gatctattta gaattcgttc cacttcctgg tttttctgct cgagttcctg aacgatattc
     2701 gccattgtga aggcaagctc accattttct ttctgacagc gaagagcgaa tgttctatag
     2761 cttccttttc tgactccttc cacttgagag cgaagtgagt ctacctgttg tttgagagag
     2821 tctctttttg cgcgagcggc tgcgggattt ccgcccttcc ctctgaccac acgagcctct
     2881 tctactgaga tctgtttttc cgcagcctca agagcgcctg acgtctgaat gaatcgatct
     2941 accagatcgc gaagaccagt ggagtcggat cgaagcgccg gatcctgttc ctgtctggct
     3001 ctctctttgt cgccgaagtt ctgagccatg cgtagataga tcgcggatag ttcattcttg
     3061 tccggattgt cctggtaagc cggatccagc gccgtgtatc gaaatgccat ggcatattcg
     3121 ttgagcgctt tggtgtgttc ttccgtcttg tggtagcaga ctcctttgag tctgtgcaac
     3181 cgatacagat atggctgtgc accgaggaaa tactctcgcc ctggctcctt gcttcgagcc
     3241 agcgcaatca acctgactgt gttttcactc aaagtctctg cggcaaagct tggattgcgg
     3301 cgttcgtcct ggatgcgttt gtctgcaatg aactgagaat agtaggacga aagcaaattg
     3361 ttcgcgcgcg tgagaatggc ttcgcagtca ggattgagtc ccagagccga cttttccagg
     3421 tcgtccacct gcgcggggtc tatgatttca gaaggctgtt tgtagagacc ctgagagaac
     3481 agggccccgg gcaaaagcaa tagaagtatg aggcgaccca tactacagtt tcggccaaat
     3541 cagcgaggag gaaagccttc gtgtctgacg gcggatacga ataagaaggc ccagcctgcc
     3601 atcagggaaa gcccgccaat cggagccact gccccaaagg cccgaacccc ggtcaaaacg
     3661 atggcataga ggcttcccga aaagacaatc gtcccaaaga gccagagccc cagaaccgcc
     3721 cgacccgatt tgcccacgcg gtccatggcc agtcccagtg ccagcatggc gatcgcatgg
     3781 tacatctggt agcgagcggc tgtttcgaaa at
//
LOCUS       DAORFF010000388         3804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14732_length_3804_cov_3.150974, whole genome shotgun sequence.
ACCESSION   DAORFF010000388 DAORFF010000000
VERSION     DAORFF010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14732_length_3804_cov_3.150974"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1779
                     /locus_tag="PKK76_14115"
     CDS             214..1779
                     /locus_tag="PKK76_14115"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNL69664.1"
                     /translation="MWISRRLQLLILTVFYSTATFAGTVTVTDTNISSVSRQTPIIAI
                     IGLGCDVGSYYTGMFLRRNEDLKGVQLGVIDGNGNPKIMTQIYDLYQKENFKEETGLA
                     IGMAVLIKNGKVVHTSEMGYPGRTHISDLSGAAPERRWIMHALAKNGIPFEGTYKPLP
                     DDWVAPESDAGAPVDLSRGRIAHYKFDDLKDSTKSQRDFIIRGGARLEKGTVFVDGKF
                     GKTAAGFMFKNRTDVFKNSFSWSLSFNPESQDSNGWSYLCKTGDSVMEVMLRDGRLLL
                     QLHPTYARGESGALGEWRFQYEEMVSFKKSHALVVSVDMEKNRVRVLLDGRRLRDVAL
                     DSKLSSQLREQEPGVDGMDLENRAGGFVFHGNADNMILYSRALNGAEMIALAREIGSP
                     MPTIVEPAKPDQSVLDLRLARAARNGDPAGIKNALASGANVNSLQSGWSPLHFAAYYG
                     HVDAAKALMDANADPLLQISGFTAQQLAHARGFAKVVILLEDYSNTTRFYHMRRFLPP
                     SQRARANPTPPGI"
     gene            1864..>3804
                     /locus_tag="PKK76_14120"
     CDS             1864..>3804
                     /locus_tag="PKK76_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNL69665.1"
                     /translation="MAVVEVTHGVALCNAAGKNILFGCPPEVIKHLMVKGLGSPEVIV
                     LPDTPYRFDTLQNCTEFPLYYFLFVERNFMQGKKLTIVGTATHLRANRKLLRLTLLGP
                     TRKEYQDLGTSHWFDELYRESRALSVKDSSGRELAIDDFVDFIPFEKGVAHLPGGIRI
                     EHTGVDRFTVGEDKIDIAFNTPQPPPYDLRNDFITTMPAHFGVTVLGGASGFISDKPC
                     SGLILNYNSDHMLIDCVPFLEYALNARGISTTEIRSIFLTHIHDDHCNIFPLLRLSNK
                     VKLLATREIFWMAMMKLSLQTLMPIEDISEMFEFVEVKPYEVTEFYGLSIETHYTVHS
                     IPTIGATFRMKDGPMSRSIVFIGDNKAFDDIETMIDQGIVRPEKFAALKQKYTERHDI
                     LFADGGMGILHGNPRDALKSQSDRIVFMHLEKLPPEFDATFSHAVAGKRYSIIEGNYN
                     SYMIHTLHILGDAFRNISHEWSTALMNNFRIVTFNAGDVIFKQNEASKGLIYVILSGS
                     CSVMVHDGFTLSERTRKEAGDFVGEMAVLDENKVRSASIVAATPVTLCALDEKLFHEF
                     LIDEDRIDEMRNLWKVRSEIERFWPFEVFADNVNDRIARNSVRTRVAAGDKIVEQGTN
                     EGEFFIILSGEFSVRRNGTEVKI"
BASE COUNT          989 a          900 c          992 g          923 t
ORIGIN      
        1 tgcctcgatc gacgcaactg aatggcaagc cacagaatga caagaagtgc aaacaggagc
       61 gcagcccagg cagtcgaagc ataggaactg aggagtgtca cccaggcgga gatattcaca
      121 aaaccgagca gacgacgacg ggtggtgtcg tccattcttt gtcgaaggct cgaaagcttg
      181 acagaaccaa atgagcggac gtcttcaggt gatatgtgga tttcgcgcag actgcagcta
      241 ttgattctta ctgtttttta ctccactgcc actttcgcgg gcacagtgac tgtaacggat
      301 acaaacattt cgagtgtttc gcggcaaacg ccgatcattg cgatcatcgg acttggatgc
      361 gacgtaggat catactacac cgggatgttt cttcgcagga acgaagatct gaaaggcgtg
      421 caacttggtg tgatcgatgg gaacggaaat ccaaagatca tgactcagat ttacgatctc
      481 taccagaaag aaaatttcaa agaggaaact ggactggcca ttggcatggc ggtactcatc
      541 aaaaacggca aagtagttca tacttctgaa atgggctatc caggcaggac gcacatctcc
      601 gacctgagcg gcgcggctcc tgaaagaaga tggatcatgc atgctctcgc gaagaatgga
      661 atcccttttg agggcacgta caaaccactg cctgatgatt gggttgcgcc agaatcggat
      721 gcaggtgctc ccgtggattt atcgcgcggt cggatcgcgc attacaagtt cgatgatctt
      781 aaagattcaa caaagagtca gagggatttt atcatcagag gtggagcgcg tctcgaaaaa
      841 ggaactgtat tcgtggatgg gaaattcggc aagactgccg ccggctttat gtttaaaaat
      901 agaacagacg tgttcaagaa cagtttttcc tggagcttga gtttcaaccc tgagtcacag
      961 gactcgaacg gctggtccta tctatgtaag acgggtgact cagtcatgga agttatgctg
     1021 agggatggta gactcttgct gcaattgcat ccgacgtatg ctcgcggaga atcgggtgcg
     1081 cttggagaat ggagattcca gtacgaagag atggtctctt tcaagaagtc acacgctctg
     1141 gtagtgagcg ttgatatgga aaagaatcgc gttcgcgtgc ttctcgatgg cagaaggctc
     1201 cgtgatgttg cgctggattc aaagctcagt tcgcagcttc gcgagcaaga acccggcgtg
     1261 gacggaatgg atctggaaaa tcgggccggt ggatttgtgt ttcacggcaa cgcggacaat
     1321 atgattctat acagtcgagc gctgaacgga gccgagatga tcgcgcttgc gcgggaaatt
     1381 ggatctccaa tgccgactat cgttgaaccg gcgaagccgg atcaatccgt tctcgacctc
     1441 aggctcgctc gcgccgccag aaacggagat cccgcgggca tcaaaaacgc gcttgcttct
     1501 ggagcaaatg tcaattccct ccagagtggg tggagccctt tgcacttcgc agcatattac
     1561 gggcacgtag acgcagcaaa agctctgatg gatgcaaacg cggacccctt gcttcaaatt
     1621 tctggtttta cggctcagca gctggcgcac gctagaggct ttgcgaaagt agtcattctg
     1681 ctcgaggatt acagcaacac cacgagattc taccatatgc gtcggttctt accgccttcg
     1741 cagcgtgctc gcgcgaaccc gactcctccg ggtatctaag attgtagata tcgaggccag
     1801 gctgtactct gttcaggtgc agaaaaggtc tggacgtcca cgtgcattgt gcgatccacc
     1861 tgcatggctg tagtagaagt cacgcatggt gtagccctct gcaatgcggc gggaaaaaat
     1921 attctgtttg gatgcccgcc agaagtaatc aagcatttga tggtaaaggg tctggggagt
     1981 ccggaagtca tcgtccttcc agacacaccg tatcgctttg acactctgca gaactgtact
     2041 gaatttccgc tctactattt tctcttcgta gaaagaaact tcatgcaggg caagaagctg
     2101 accatcgtcg ggaccgctac gcatcttcgc gcgaatcgca aactgctccg gctgacgctt
     2161 cttggcccga cgcgaaaaga atatcaggat ctggggacaa gtcactggtt tgacgaattg
     2221 tacagagaat cgcgcgctct ctctgtgaag gattcttccg gtcgagagct tgccattgat
     2281 gattttgtgg atttcatccc attcgagaaa ggcgtcgcgc atcttcccgg cgggattaga
     2341 atcgagcaca ctggagttga tcgctttaca gtaggcgaag acaaaataga cattgccttc
     2401 aacactccac agcctccgcc atacgatttg cgaaatgact tcatcacaac catgcccgct
     2461 cattttggcg tgactgttct tggcggagct tctggattca tttccgataa gccatgttcc
     2521 gggttgatct tgaattacaa ttcagaccat atgttgatcg actgcgtgcc gttcctcgaa
     2581 tacgcgctga acgctcgtgg tatttccacc accgaaattc gatccatttt tctaacgcac
     2641 attcacgatg atcactgcaa tatctttcct ctgttgcgtc tctcaaacaa agtgaaactt
     2701 ctggccacgc gagaaatttt ctggatggcc atgatgaaac tgtctctgca gacgctcatg
     2761 cccatagaag atatttcaga gatgtttgag tttgtggaag tcaaaccata cgaggtcaca
     2821 gaattctacg gtctctctat tgagacgcac tacacagtgc actccattcc aacgattggc
     2881 gcaacattca ggatgaaaga cgggccaatg tctagaagca ttgtgtttat cggagacaac
     2941 aaagcgttcg atgacattga aaccatgatc gatcaaggaa ttgtaagacc tgagaagttc
     3001 gcagccctca agcaaaaata cacggaacgt cacgatattc tttttgcaga cggcggcatg
     3061 ggaattcttc acggaaatcc acgcgatgcg ctgaagtctc aatcagatcg cattgtattc
     3121 atgcatctgg aaaagcttcc gccggaattc gacgccacgt tctctcacgc cgtggcagga
     3181 aagagataca gcatcattga aggaaactac aattcctata tgatccatac tctacatatt
     3241 ctcggagacg cattccggaa tatttcgcat gagtggagca cagctctcat gaacaatttt
     3301 cgcattgtga cgttcaatgc gggagacgtg atcttcaaac agaatgaagc gtccaaaggg
     3361 ctgatttacg tcattctctc cggatcctgc agcgtgatgg ttcatgatgg gttcaccctt
     3421 tcagagcgga ctcgaaaaga ggccggcgat tttgtgggag agatggccgt tctagatgag
     3481 aacaaagtca gatctgcttc tattgtagcc gcaactccgg taacgttatg cgctctcgac
     3541 gaaaagctgt ttcatgaatt cctcatagac gaagatcgaa ttgatgaaat gcgcaatctc
     3601 tggaaggtgc gttcggaaat tgaacgattc tggcctttcg aagtgttcgc agacaatgtg
     3661 aatgaccgca tcgcgcgaaa ctcggtgcgt acccgcgtgg ctgccggaga caaaattgtc
     3721 gagcagggca caaatgaagg ggaattcttt atcattttgt cgggtgagtt cagtgttagg
     3781 cgaaacggta cagaagttaa gatt
//
LOCUS       DAORFF010000389         3772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14932_length_3772_cov_4.099812, whole genome shotgun sequence.
ACCESSION   DAORFF010000389 DAORFF010000000
VERSION     DAORFF010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14932_length_3772_cov_4.099812"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..631)
                     /gene="thpR"
                     /locus_tag="PKK76_14125"
     CDS             complement(110..631)
                     /gene="thpR"
                     /locus_tag="PKK76_14125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNL69666.1"
                     /translation="MRLFVAIPIPEEVQDDLGGLTGSLAGVKWTDPQNIHLTLHFLGE
                     DFEPEEIHSALASVHSGPFPVEVKGVGRFLHNQGGAIWAGVLPSPQLMELHARIGKAL
                     RAVSVPTEKRAYQPHITLGRVKNEPEHRIQDYILHHQDFHTSFKAVSFALFSSRLRPD
                     GPIHSIDMEYEFD"
     gene            766..1551
                     /locus_tag="PKK76_14130"
     CDS             766..1551
                     /locus_tag="PKK76_14130"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNL69667.1"
                     /translation="MEYYLICALIFGVAYLVNATYISIFYHRGLTHSAIELKPGLRKF
                     VVASGPWVMGIDAKTWCTMHRLHHRHSDKESDPHSPLNSSIPRVIVEQLKSYERIMRG
                     LIARKKQYTSEVPDLDFNVHWLYRNKLWWLPYALHLAISVGLSFALGGWLVGLAFYSG
                     MVTHPIQGWLVNSFGHSMGYQTFDNGDNSRNNNIVSWLVMGEGYQNNHHKHPRSPKFS
                     YRWFEVDYGYRICQAMQALGMITIEKDYLIRVVERRELEERAA"
     gene            complement(1569..3005)
                     /locus_tag="PKK76_14135"
     CDS             complement(1569..3005)
                     /locus_tag="PKK76_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69668.1"
                     /translation="MRRISPSRLTGLALVIASSLILAFSSRPLHAQESSTPIADRFSY
                     RGDMFIRGIYLARDLPLKSQSYNTTSRNREEQDFYSARLRLDLAFRASQYVDVLYGLE
                     VGELTFGRDNASRAGPGSGGKGAGAANLETRELILKFRNYPESLSFSAGILNFDTPRG
                     LVTGTSGAGAKFSAEMRALHSSVDAYYIRSEDNSTLDGDSNGFSDSNFRNVNMGLISW
                     KFSGIPGMRSEFYGMYRQDDDPSLTAGDSGETSRVYWGGIFTQYKAGQFGLMLHGVGN
                     WGNFWRPGATSTSIRKRYTINAGAGQYEVSWQFLDQLQVALVGAGASGRLGHEPNASS
                     SEFRTDQFRTNSSAFQFTSIALDSSGGYTIFPGGKLTGIVAHGIEFRSTIFNVLQADF
                     SAYLIQLYRSPVVDYNAYYTRYPALHRSDRRLGKEYDAKFAWRFLSDLTLEAKLAYFD
                     ADDGYRIINDVVYGDDMAEIQISATQKF"
     gene            complement(3009..>3772)
                     /locus_tag="PKK76_14140"
     CDS             complement(3009..>3772)
                     /locus_tag="PKK76_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNL69669.1"
                     /translation="RLLDIKKSGKIKIGIQNEYRPFRIENPKPGYPGIDVELANALGE
                     ALEVEVEISYLPLDQLLIETNEGKLDVSLGGISSTLERARKVNFSDPYMKTTPAGLLA
                     RDSLPPESESVNYARRQFKSLSDLKYLGPITIGVRAGTANETLLRTDPDFQKHKIKVF
                     QDRTIALAALESREIDLLVADDVYLRALLLKRPELMNRFLPLLDKYLEDHISMAIPPG
                     DPELWNYLNFFIKEMIRTGRMQTIINKYFQSGAWF"
BASE COUNT          909 a          902 c          959 g         1002 t
ORIGIN      
        1 atagaagagt tccatttcca gccagtagcg ggccggtagc tgcgcaaacg gcgacgagaa
       61 cgatgacgga gcgcagcatg gctcgatttg aaatcggggc cggataactt caatcgaatt
      121 cgtattccat gtctatggaa tggatcgggc catccggtct cagtctgctc gagaacaatg
      181 cgaaagacac ggctttgaag gaagtgtgaa agtcttgatg atgcaatatg taatcctgga
      241 ttctgtgttc tggttcgttc ttcacccggc ccagcgtgat gtgaggttga taggcgcgtt
      301 tttccgttgg aacagaaaca gcgcgaagag ccttgccgat tctcgcgtgc aattccataa
      361 gttgcggcga tggtagaacg ccggcccaga tggccccgcc ctggttgtgc aggaatcgac
      421 ctaccccttt cacttccacc ggaaaaggac cggagtgaac ggaggcaaga gcggagtgga
      481 tttcctcggg ttcaaagtcc tctccgagga aatggagggt gagatggatg ttctgtgggt
      541 cggtccattt tacgccggcg aggctgcctg taagccctcc tagatcgtct tgaacctctt
      601 ccggtatggg aatggcgaca aatagacgca cggcgcccat ttttccgcat tgggcagaat
      661 tctgcactcg atttaggtcc aggatgagga aagggcttgt cagcgccgga gtaagaaaaa
      721 acatggtgta tatctaacca tgttagatat gcacggaggg ttatcgtgga atactatttg
      781 atttgtgcct tgatttttgg cgtcgcgtat ctggtgaatg cgacctatat ttctatcttc
      841 taccatcgcg gattgactca cagcgccatt gagctcaagc cgggactgcg caagttcgtc
      901 gtggcaagcg gtccgtgggt gatgggaatc gatgcgaaga cctggtgcac gatgcacaga
      961 cttcaccatc gtcactcaga taaagaaagc gatccgcaca gtcctcttaa ctcaagcata
     1021 ccacgcgtga tcgtggagca actcaagtcc tacgaacgga tcatgcgcgg actcatcgca
     1081 aggaaaaagc aatacacctc cgaggtgcct gaccttgatt tcaacgtgca ctggttgtat
     1141 cgcaacaagc tctggtggtt gccttacgct ttgcaccttg cgatttcagt tggtctttct
     1201 ttcgctctgg gcggatggct ggtaggcctt gcgttctatt ctggaatggt tacccatcca
     1261 attcaaggct ggctcgtaaa ttcttttggt cattccatgg gataccagac gtttgataac
     1321 ggagataact caagaaacaa caatattgtt tcatggcttg ttatgggtga aggatatcag
     1381 aacaatcacc acaaacatcc tcgctctcca aagttttcct atcgctggtt tgaagtggat
     1441 tatggttatc gcatttgcca ggcaatgcag gccctgggta tgattaccat tgagaaggat
     1501 tatttgattc gcgtagtaga aagacgcgaa ctggaagagc gcgctgcctg attgagggct
     1561 gtgttcgatc agaatttttg cgtggccgag atttgaatct cggccatgtc gtctccatag
     1621 acaacatcat tgataattct atagccatca tctgcatcaa agtaggccag ttttgcctcc
     1681 agagtcagat ccgacaaaaa tctccacgcg aacttcgcat catattcttt gcccagtctt
     1741 ctatctgatc tgtgcaatgc cggataacgg gtgtagtacg cattgtagtc cacaactggc
     1801 gagcggtaca attgaatcaa ataagcggaa aaatctgcct gcagtacatt gaagatagtg
     1861 gatcgaaact cgattccgtg cgctacgatt ccggtcagct ttccgcctgg aaagattgtg
     1921 tatcctccgg aagaatccag ggctatagaa gtgaattgga atgcagatga attcgtgcga
     1981 aactgatctg tgcgaaattc gcttgagctg gcattcggtt catgcccgag tctgccggat
     2041 gctcctgctc ccactagcgc cacctgaagt tgatcgagga attgccacga aacctcgtac
     2101 tgtcctgctc cagcattgat cgtgtacctt tttcttatgg acgtcgaggt tgccccggga
     2161 cgccagaagt ttccccagtt cccgacaccg tgcaacataa gcccgaactg tcctgccttg
     2221 tactgagtga atattccgcc ccagtagact ctcgatgttt cgccgctgtc tcctgcagtc
     2281 agagatgggt catcgtcctg tctgtacatt ccgtagaatt cgcttcgcat gccgggaatt
     2341 ccggaaaatt tccaggaaat gagtcccata ttgacgttgc gaaaatttga atctgaaaaa
     2401 ccatttgaat ctccgtccag ggtggaattg tcctcagatc gaatgtagta agcgtccaca
     2461 gatgaatgaa gggcacgcat ttcagctgaa aattttgcac cggctccgct tgtgcctgtc
     2521 accagtcccc tgggtgtatc aaagtttaga attcccgcgc tgaatgaaag agattctgga
     2581 tagttacgaa attttaggat caattccctg gtctccaggt tcgcagctcc agcacccttt
     2641 ccacctgatc caggtccggc tctcgacgca ttgtctcttc caaatgttag ttcgcctact
     2701 tcaaggccgt agagtacgtc cacatattgc gatgcgcgaa aagccagatc caatctgagt
     2761 ctggcggaat agaaatcctg ttcttctcga tttctggatg ttgtattgta ggactgcgac
     2821 ttgagcggga ggtctctggc cagataaatc ccgcgtatga acatatctcc gcgatagctg
     2881 aaacgatctg cgatcggtgt gcttgattcc tgcgcatgaa ggggccgcga agaaaaggca
     2941 agaatcaaag aggaagcgat aaccagcgcc agacccgtca ggcgggatgg tgaaatccta
     3001 cgcatttgct agaaccacgc accgctctga aaatatttgt tgatgattgt ctgcattcgc
     3061 cccgtacgaa tcatttcttt aataaagaag tttaagtaat tccagagctc tggatctcct
     3121 ggtggaattg ccatgctaat gtggtcttcc agatacttgt ccagcagtgg aagaaaacga
     3181 ttcatcaact ctggtcgttt gagaagaaga gcgcgtaaat acacgtcatc ggcgaccaga
     3241 agatcgattt ccctggattc cagtgccgcc agggctatag ttctatcctg gaagactttg
     3301 atcttgtgtt tttggaaatc aggatctgtt ctcaaaaggg tttcgtttgc agtacctgcg
     3361 cgcactccga ttgtgatcgg acccaggtat ttcaggtcag acaggctttt gaactgtctg
     3421 cgcgcataat ttacggattc agattccggg ggcaaagaat cgcgcgccag aaggccggcg
     3481 ggtgttgtct tcatatacgg atcgctgaaa tttacttttc gcgcacgttc cagtgtagaa
     3541 gagattccac caagacttac gtccagctta ccctcgttcg tttctatcag gagctggtcc
     3601 agaggtagat aagaaatttc aacttctact tccagagctt ctcccagtgc gtttgccagt
     3661 tccacatcaa tgccaggata tcctggtttt ggattttcaa tgcgaaaggg tctgtattcg
     3721 ttttgaatcc cgatcttgat cttcccggat tttttgatat cgagaagtcg tg
//
LOCUS       DAORFF010000390         3768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14957_length_3768_cov_3.266900, whole genome shotgun sequence.
ACCESSION   DAORFF010000390 DAORFF010000000
VERSION     DAORFF010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14957_length_3768_cov_3.266900"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PKK76_14145"
     CDS             complement(<1..756)
                     /locus_tag="PKK76_14145"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803965.1"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HNL69670.1"
                     /translation="MEIPSYVKNQKLIAWVNEVAALCKPERVYFCDGSQEEYDRLCDQ
                     MVQAGTMVRLNPEKRPKSFLALSHPSDVARVEDRTYINAKRKADAGPTNNWVDPAEMK
                     QTLNKLYDGCMTGRTMYVIPFSMGPLGSDIAHIGVEISDSPYVVVNMRIMTRMGKAVL
                     DVLGDRDFVKGLHSVGAPLKAGEKDVPWPCNHDTKYICHFPEENLIMSYGSGYGGNAL
                     LGKKCFALRIASTMARDQGWLAEHMLILGVQDPQ"
     gene            1161..1532
                     /locus_tag="PKK76_14150"
     CDS             1161..1532
                     /locus_tag="PKK76_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HNL69671.1"
                     /translation="MRFISVLFVPILLVASCTGLGINTHVGLNSNPAPEYGNLAVLVA
                     RGGMFYHQNGVPGPKGNAAATKSGRGCNYSVLWLAAWGDSSIDSIREDSGISKVGSVD
                     YDETAILGFFFHRFCTTVMGE"
     gene            1539..1856
                     /locus_tag="PKK76_14155"
     CDS             1539..1856
                     /locus_tag="PKK76_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNL69672.1"
                     /translation="MLIKQFTSFLILTLAVSSCAVGPVNGVLFTRTKFAGQINSAADV
                     PVQKKASGCMHQVLGTVAWGDATAGTVAIENDIHRIAIIDHSTMSILSGVYSNYCTIV
                     AGD"
     gene            1867..2196
                     /locus_tag="PKK76_14160"
     CDS             1867..2196
                     /locus_tag="PKK76_14160"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNL69673.1"
                     /translation="MSKWFLTFLPLLLLSACISSPLQGWLITYTGSHSSMVDKGAISS
                     AKIVKKGESCSWSAWIFKDIYYGGGGDVKEAADSANIKKIAVVDRRSFSLLFDLVFKE
                     CVLVYGE"
     gene            2199..2996
                     /locus_tag="PKK76_14165"
     CDS             2199..2996
                     /locus_tag="PKK76_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69674.1"
                     /translation="MSLQWRTFIIAAALLAASRVHAQSDELYRKDRLYVDIAYGTGPT
                     TVDFVAAGFGNASYTAALVDLSLGNQNNRNLALLYFLTKAGKNPNVSMSGSQRIGIEY
                     AATSWLTFGVGASHFTGTISGGGLNQLDGLGNLGYLIMLYNYTSVGSSSGNSLSSTLY
                     TLPYSVSINTSIAEIQYAVHVQAGFADPYFRMSGGSAKGISKSEIAFGSRFRFAGNAY
                     VYTEAFYTQYALNSDLTQSTIAATVVNKGARFGVGYAFDPPDTIASK"
     gene            complement(3024..>3768)
                     /locus_tag="PKK76_14170"
     CDS             complement(3024..>3768)
                     /locus_tag="PKK76_14170"
                     /inference="COORDINATES: protein motif:HMM:NF013859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69675.1"
                     /translation="DSSPFHPHGISLFAGKSETLLYVVAHPDEEHNAIEVFRVLPDSL
                     AFKERLTSEVLVHPNDVASLANGELYVTNDHGWTGGLAVLEDLFALPRSTVAHYREGK
                     WSFVADGFRVANGIAVNRALDRLYVGATRDRGIHVYVRNPADGSIGKELDFIDMGSGT
                     DNVMWESDEILDVAGHYSMIRFIRTVKDPTALSPNDITRVNVTSGKKEMIFATDGSEI
                     SGASTGLVLKGKLYISQVFEPFILSCDMK"
BASE COUNT          923 a          951 c          954 g          940 t
ORIGIN      
        1 ttgtggatcc tgcacaccaa ggatgagcat gtgctccgca agccagccct gatcgcgcgc
       61 catagtggac gcaatgcgaa gagcaaaaca ctttttgccc agaagagcgt ttccgccgta
      121 tccggatccg taggacatga tgagattttc ttctgggaaa tggcagatgt attttgtgtc
      181 gtgattgcaa ggccatggaa catctttttc gcccgccttg agtggagcac ccactgaatg
      241 gaggcctttt acaaaatcac gatcccccag aacgtctaga actgcttttc ccatgcgcgt
      301 catgatgcgc atgtttacga ccacgtaagg tgaatctgag atctcaacgc cgatgtgggc
      361 aatgtcagat ccaagaggtc ccatgctaaa cggaatgaca tacatggtgc gtccggtcat
      421 acaaccgtcg tagagcttgt tcagcgtttg cttcatctca gccgggtcca cccagttgtt
      481 ggtgggacct gcatctgctt tgcgctttgc attgatataa gtacgatctt ccacacgcgc
      541 cacatcgctt ggatgcgaga gtgccaaaaa ggacttaggg cgtttctccg gattcagacg
      601 gaccatggtc ccggcttgca ccatctggtc gcaaagcctg tcatactctt cctgggaacc
      661 gtcgcaaaaa tagacgcgtt ccggcttgca gagtgctgct acctcattca cccaggcgat
      721 gagcttctga ttcttaacat aggaaggaat ttccattttc ccaaatctcc tttttaccga
      781 tccggcacaa cccgtgcccg agccggcctg aaaatatcag actggaagca ggcggttttc
      841 agtccagtag ctttcgagtc agaaaaaagc gactccgggc ggggcgaatc ggcgacctgt
      901 catggacaga acgacgcacc tgctggtgcg gcctccagtt cgggcgcgcg tcgaggcgcc
      961 ctggcgatcg aaatcaaact cagcgcctga gaatcagaat cacgaatgaa gcatcgggat
     1021 ttcaatttct gtggaatgaa ctacatgaat caggttatga aacacacttt ttgctgacac
     1081 aatgaaattc aacgaaatct gtttgaccga ttcatgtgca agatcgtgca gacgactatg
     1141 aaacttgagg tttacttact atgcgtttta taagcgtgtt gtttgtgccg attctactcg
     1201 ttgcatcgtg caccggtctt ggaatcaaca cacatgtcgg actgaattcc aaccctgcgc
     1261 cagaatatgg caacctcgcg gtgctagttg cgcgcggtgg catgttttat catcagaatg
     1321 gtgtacctgg tccgaagggc aacgctgctg ctaccaaatc ggggcgcggt tgcaactact
     1381 ccgtgctttg gcttgctgca tggggagact caagtataga cagcattcgc gaagacagcg
     1441 gaatcagcaa agtgggttcc gttgattatg atgaaactgc aattcttgga ttcttctttc
     1501 atcgattctg cactacagtg atgggggaat aatagtatat gttgatcaag caattcacct
     1561 cattcctgat tctaactctc gccgtttcaa gctgcgcagt tgggccagtc aacggtgtcc
     1621 tcttcacacg cacaaagttt gccggacaga tcaatagcgc agcggacgtt ccagtgcaga
     1681 agaaagcttc tggctgcatg catcaggtgc tcggcactgt cgcctggggc gatgctactg
     1741 caggcacggt tgcgattgaa aatgacatcc acagaatcgc aatcatcgac cattctacca
     1801 tgagcatcct atccggggtc tactccaact attgcacaat cgtggcgggt gattgaaatg
     1861 aaaacaatga gcaaatggtt tcttacattt ctacctctgc tgctgctgag cgcttgcatc
     1921 agttctcctc tgcaaggctg gctgatcaca tacactggaa gtcactccag catggtggac
     1981 aaaggtgcga tctcttctgc aaagattgtg aagaagggag agtcctgttc ctggtctgcg
     2041 tggatcttta aggacatcta ctacggtggt ggtggagacg tgaaagaggc agcggattct
     2101 gcaaacatca agaaaattgc ggtggtggat cgcagatcat tctcactgct gttcgatctt
     2161 gtcttcaaag aatgtgtcct ggtctacgga gaatgatcat gtctctgcaa tggcggacat
     2221 tcatcatcgc ggcggcgctc cttgcggcgt cccgggtgca cgcgcaatcg gatgagctct
     2281 accggaaaga caggctctac gtcgacattg cgtatggaac cgggcctact acagtagatt
     2341 tcgttgcagc ggggttcggc aacgcaagct acacggctgc actcgtagat ctttcgctgg
     2401 gaaaccaaaa caatcgtaac ctggccctgc tctatttttt gactaaggcg ggaaagaatc
     2461 cgaatgtgtc catgagcgga agccagagaa ttggcatcga atacgcggct acgagctggc
     2521 taacattcgg cgtcggggca agccacttca ccggaactat ttcggggggc ggtctcaacc
     2581 aactggacgg cctcggaaac ctgggttatc tgatcatgct gtataactat acgagcgtgg
     2641 gctccagttc cggaaatagc ctgtcctcca cgctctacac tctgccgtat tctgtttcga
     2701 tcaatacgag catcgcggaa attcagtatg cagtgcatgt tcaggccggc tttgcagatc
     2761 cttactttcg catgtccggt ggatctgcga agggaatctc aaagtcagag atagcatttg
     2821 gctccagatt ccgttttgca gggaatgcgt acgtgtatac ggaagctttc tatacgcagt
     2881 atgcgttgaa ttcggacctg actcagagca caattgcggc cacagttgta aacaaaggcg
     2941 ctcggttcgg ggtgggttat gcgttcgatc cgccagatac gattgcctct aaataattgc
     3001 cgcaaacagg aaagaagagc cgctcatttc atgtcgcagg atagaatgaa cggctcaaat
     3061 acttgagaaa tatagagttt tcctttcaat acgagtcccg tggacgcacc actgatctct
     3121 gaaccgtcgg ttgcaaaaat catttctttc tttccggatg taacgttcac tcgagtgata
     3181 tcgttgggag aaagtgcagt tgggtctttc acagttcgga tgaatcgaat catgctgtaa
     3241 tgccctgcca cgtctaaaat ttcatccgac tcccacatca cgttgtctgt ccctgaaccc
     3301 atgtcgatga aatccagttc ctttccaatg gacccatctg ccggattgcg aacatacaca
     3361 tgaatgcctc tgtctctggt ggcgcctacg tacaaccggt cgagtgcacg attcaccgca
     3421 attccatttg caacgcgaaa gccatctgcg acaaaactcc acttcccttc gcgatagtga
     3481 gcgaccgttg atcgagggag ggcaaataag tcttcaagaa cagccagtcc tcccgtccag
     3541 ccatgatcat tggtaacgta tagctctccg tttgcaagac tggccacatc gtttggatga
     3601 accaggactt cagacgttag gcgctcttta aaggcaagag aatctggaag aacacgaaac
     3661 acttcgatcg cattgtgttc ttcatccgga tgagccacaa catacagcag cgtttcgctt
     3721 tttcccgcga acaatgaaat tccatgagga tgaaatgggg acgaatct
//
LOCUS       DAORFF010000391         3766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14972_length_3766_cov_3.332794, whole genome shotgun sequence.
ACCESSION   DAORFF010000391 DAORFF010000000
VERSION     DAORFF010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14972_length_3766_cov_3.332794"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /gene="ligA"
                     /locus_tag="PKK76_14175"
     CDS             complement(<1..1779)
                     /gene="ligA"
                     /locus_tag="PKK76_14175"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNL69676.1"
                     /translation="MASQAEARMRKLEAEIRRHQNLYYVQHKPLISDRQFDRLLEELS
                     SLEKENPGLASPDSPTRLVGSDLDNSFPKFKHTIPVLSLANTYSTEEAVAWAEKTCEK
                     KETILIEWKVDGATLVLYYEKGKLVRAVTRGTGQVGDVVTENARTIRSIPDRLSEEID
                     LTVRGEAYMNFDEFDAFNEQQGNIYANPRNLVSGSLKHKKSRETALRPIRWVAFEAYF
                     AKDEKLKTDSSQLDRLSQLGLPVFESHTVSSSGLAAKIELLSKKVKKLNFPVDGLVLK
                     VDDLNLRRDLGYTSASPRWATALKFEPELAQTTVEEIEVFTGRTGRVTPRARLVPVKL
                     AGTTVTYATLHNADYIEKLGVRVGSTVKISKRGEIIPAVEEVVDQGKGPPFSFPKKCP
                     SCGSKLVRGEEAADWLCPNEECDEKLMNAIVFFCQRKQMDIAGLGERNVEILFRKGFV
                     KTIPDLYRLGERKEELEAMEGFGSKSVQIILEGIEASKKQEYRRVLYSLGLREIGPSV
                     TDILLDHGLHSIDDLINAARAKGAVEALQEIHGIGPSTAEAIVEQLNDKRVLAMIDSL
                     RQAGLQFSQKIEKSNLQQIFEGQTWCV"
     gene            complement(1783..2208)
                     /locus_tag="PKK76_14180"
     CDS             complement(1783..2208)
                     /locus_tag="PKK76_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNL69677.1"
                     /translation="MGLKITNQRLLVAESIFRHPSHFTVDSLAESLKDRRDEISRATI
                     YRIVSILVESGQLTEHNFAQGVRYYEHIPRHEHHDHIVCQDCGRIEEFLVPEIEKVQQ
                     QIAEQHGFALEAHALNLYGRCAELKTKGSCARRLEKTPQ"
     gene            complement(2279..3166)
                     /gene="thiL"
                     /locus_tag="PKK76_14185"
     CDS             complement(2279..3166)
                     /gene="thiL"
                     /locus_tag="PKK76_14185"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNL69678.1"
                     /translation="MLSESQIIELFQKDSYLLDDCAAIPTNGEINLVSTDSLSEGTHF
                     RRDWSSPEDLAIKLLHVNISDLHSSGAKPSWCFLNLGLPSIEDDFIRRFARAFREERM
                     ACGIDLLGGDTFRSSELQLQLTMGGYAKRHVMRSGGRPGDVLYVTGTFGLSLAGYLHL
                     SGQVDAGGMASLAVGRHLRPRSHPYVDLTHVHAAMDVSDGIAQDSARLAKASRVRLEI
                     DLEKIPVAAGLKITAEEAVVSGEELELLFLADANFSDFLAVPIGVARSGEGCVFMRQG
                     DPVEIGTGFSHFHAVDSSD"
     gene            complement(3168..>3766)
                     /locus_tag="PKK76_14190"
     CDS             complement(3168..>3766)
                     /locus_tag="PKK76_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69679.1"
                     /translation="VISDDPMFRLRMRQILRFAGREPRMDFSATEDIAQVLTHIQEWP
                     EIIVVDLDSSRVDVVEFIYRTEKILRERPAMRTRNQMMIVKDFAKPGLDPVKLRPVLS
                     PHAKRIFHPLEAMLAILESMVFYSGEVEPLVDEHPRSLREMLYGDDFAPPGRLSESFL
                     AGLKSRGTALSRSSQFILFAQHLKDRLIETGSGSLAMK"
BASE COUNT          918 a         1002 c          874 g          972 t
ORIGIN      
        1 cacgcaccac gtctggcctt caaaaatttg ctgtagattt gatttctcga ttttctgcga
       61 gaattgaagc cctgcctgtc ttagagaatc gatcatggca agaactcgtt tgtcgtttaa
      121 ctgctctaca atcgcctccg cagtgcttgg gccgatccca tgaatttcct gcaaagcttc
      181 tacggcacct tttgcgcgcg ctgcgtttat aagatcgtca atgctatgaa gcccatggtc
      241 caggagaatg tctgtgacac ttggacctat ttcgcgcaac cccagtgaat aaagcaccct
      301 gcggtattcc tgcttcttag atgcttcgat tccctctaaa atgatttgca ctgatttgga
      361 tccaaaccct tccatggcct cgagttcttc tttgcgttcg cctaaccggt ataagtcggg
      421 gattgtcttc acaaatcctt tgcgaaatag aatctcaacg ttacgctctc caagtcccgc
      481 aatatccatt tgctttctct gacagaaaaa gacgattgcg ttcattagct tttcgtcgca
      541 ttcttcgttt ggacaaagcc agtctgccgc ttcctcaccg cgtacgagct ttgagccgca
      601 ggagggacat tttttaggaa aagagaaagg cgggcctttg ccctgatcta caacttcttc
      661 gactgcagga atgatttcgc cgcgcttgga aatcttgacc gtggaaccaa cacgcactcc
      721 gagtttttca atgtagtccg cattgtgaag cgtagcatac gtaacggttg tgcctgccag
      781 ctttacagga accagcctgg ccctgggagt caccctcccg gttctacccg tgaatacttc
      841 aatctcttct acggttgtct gtgcaagctc gggttcaaac ttaagagctg tggcccatct
      901 gggggaagca gaagtatatc ccagatcacg acgcaggttc aggtcgtcta ctttcagaac
      961 aagaccatcc acgggaaagt ttagcttttt aactttttta cttaacaatt ctatttttgc
     1021 cgcgagacca gaagaagaca ctgtatggga ttcgaacact ggaagcccca gttgggaaag
     1081 acgatccagt tgcgatgaat ccgtttttag tttttcatct ttcgcgaagt atgcttcaaa
     1141 ggcaacccag cgaatgggtc gaagagcagt ttcgcgggac ttcttatgct tcaaagaacc
     1201 agatacgaga ttgcgtggat tggcgtagat atttccctgc tgctcgttga atgcatcaaa
     1261 ttcatcgaag ttcatgtacg cttctccgcg tacagtcaga tcaatctctt cgctcaggcg
     1321 gtccgggatg ctgcgaatcg tgcgtgcatt ctcagtgacc acatctccca cctgccccgt
     1381 tccgcgtgta acagcgcgaa cgagctttcc cttctcatag tagagcacaa gtgtggcacc
     1441 atcgactttc cattcgatga gaatggtttc ctttttctcg caggtcttct ctgcccacgc
     1501 aactgcttcc tctgttgaat aggtattcgc cagagagagt actggaattg tatgcttgaa
     1561 cttcgggaaa gagttatcca gatcacttcc aaccaggcga gtgggtgaat caggcgaagc
     1621 aagtccggga ttttcttttt cgagagaact gagctcttcg agcaaccgat caaactgtct
     1681 atctgagatg agaggtttat gctggacgta gtacaaattc tggtggcgac gaatttccgc
     1741 ctccagtttg cgcatgcgcg cttctgcttg tgaggccatc ttctactgcg gagttttttc
     1801 aagacgacga gcacaggagc ccttcgtctt gagttctgca catctgccgt acagattcag
     1861 agcatgagct tccagagcga atccatgttg ttcagcaatc tgctgttgca ctttttcaat
     1921 ttccggaacc aggaattctt caattctacc gcaatcctga cacacaatgt gatcatggtg
     1981 ctcatgtctg ggaatgtgtt cgtagtatcg cacaccctgt gcgaaattat gctctgtgag
     2041 ttgaccggat tctacaagaa tagaaacgat tctgtaaatg gtggcgcgcg aaatctcgtc
     2101 tcttctgtcc ttcagagact ccgcgagcga gtctaccgtg aagtgagatg gatgtctgaa
     2161 aatagactcc gctaccaata gcctttgatt ggtaatcttt agccccatct gcttgaggta
     2221 ttccgagaag atggaaagtt ctttgtgaac gtctttgacc acatcccttc tttacggatc
     2281 agtcagagga gtcaaccgca tgaaaatgag aaaacccggt tccgatttcg actggatcgc
     2341 cttgccgcat gaatacgcag ccttcgcctg aacgtgccac cccaatggga acggccaaaa
     2401 aatcgctgaa attcgcgtcc gccaggaaca aaagctccag ctcttccccg ctcacaacag
     2461 cctcttccgc ggtgattttt agcccggctg caacgggaat cttctccaga tcgatctcca
     2521 ggcgaaccct ggacgctttt gcaagcctgg cgctatcctg tgcgattcca tccgaaacat
     2581 ccatggcggc gtgcacatga gtcaagtcca catacgggtg agacctgggc ctgaggtggc
     2641 gcccgacagc cagagacgcc atcccgcccg catccacctg accgctcaga tgaagatatc
     2701 cggcaagcga cagaccaaac gtcccggtaa catacaaaac gtcccccggc ctaccgccag
     2761 accgcataac gtgccgtttg gcgtagccac ccatggtcag ttgcagctga agctcagacg
     2821 atcggaatgt atctcccccg agcaaatcaa tcccgcaggc catcctctcc tcgcgaaagg
     2881 cacgcgcgaa gcggcggatg aaatcgtctt cgatcgacgg caaccccaga ttcaggaaac
     2941 accaggaagg ctttgcaccg ctcgaatgca gatccgaaat attgacgtga aggagtttga
     3001 tggcgagatc ttccggagaa gaccagtccc ttctgaaatg agtaccttcg ctcaggctat
     3061 ctgttgaaac aaggttgatt tcaccgttag tcggaattgc ggcacaatcg tccaacagat
     3121 acgagtcttt ttgaaaaagc tctatgatct gggattcgct gagcatatca tttcattgcc
     3181 aggctgccgg aacctgtttc tatgagccta tctttgagat gctgggcaaa cagaataaac
     3241 tgagacgatc tggacaatgc ggtccctctt gatttgagcc cggccagaaa tgattccgac
     3301 agtcttccgg gcggtgcaaa atcgtcaccg tacagcattt cgcgaagcga tcttggatgc
     3361 tcatcgacaa gtggttccac ttctccacta tagaacacca tcgattcaag aatggcaagc
     3421 attgcctcca gcggatgaaa aatcctcttt gcatgcggag aaagaacagg acgtagtttc
     3481 accggatcaa gccctggctt cgcaaaatcc tttacaatca tcatctgatt cctggttcgc
     3541 atagccggtc gttctctcaa aatcttttcc gtacggtaaa taaattcaac tacatccact
     3601 cgacttgaat ccaaatccac gactatgatc tccggccatt cctgtatatg agtcagaacc
     3661 tgtgcaatgt cttccgttgc cgaaaaatcc atcctgggtt cgcggcctgc gaaacgaaga
     3721 atctgccgca tgcgaagacg aaacatggga tcgtctgaaa tcacaa
//
LOCUS       DAORFF010000392         3763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_14989_length_3763_cov_3.478964, whole genome shotgun sequence.
ACCESSION   DAORFF010000392 DAORFF010000000
VERSION     DAORFF010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14989_length_3763_cov_3.478964"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PKK76_14195"
     CDS             complement(<1..994)
                     /locus_tag="PKK76_14195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNL69680.1"
                     /translation="MALRYIAPFVLAAVIFSLDLMVPRGFAVWLWYIPALFVSRPHGK
                     LLAIEVWIFSVLMLGGAMMSPESSVPVAVVFANRSLGIALMAVVALLLYTGGRMNEAL
                     RLAHQRAEEASEAKTRFLGYVSHDMRTPLNAIIARLDVIKADAQTAHVLRDLNYIESH
                     ASFLSAFIHELLDYANGNENEIEIAPVRLREVFDSIAALFQPLIPEGVSLDISVSSEL
                     EFPTDKRRLQHILLNLIANSVKFTQFGSISVYAAESPAGLHLTIDDTGSGVSQAMLEK
                     MFEHGITGDPDGLGLGLAMTKYFVRFLGGYVSAFSDGPGAGLSVEILIPHPDRRT"
     gene            complement(985..1722)
                     /locus_tag="PKK76_14200"
     CDS             complement(985..1722)
                     /locus_tag="PKK76_14200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL69681.1"
                     /translation="MIPFYRILLVEDDPGFAESVMRVFAAFNILLDSATDGRVALIWV
                     RSRPYDAVITDLRMPIVGGLAFIESATAFLTGKPVLVMTASNQREDVLSARELKVDDY
                     ITKPVKPSELVLRLARKLPATPGLVPLQRFPLQIHYKSLGSAGVSISLEGTPGSDFEK
                     AFGDMLADLRNHLGTDLASVEFQVASEFGYDPRSIYALEKALDLLSRRLRVPGPRIHL
                     SGGYWDDARETHGDEARRGILGSVRWP"
     gene            1953..3020
                     /locus_tag="PKK76_14205"
     CDS             1953..3020
                     /locus_tag="PKK76_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNL69682.1"
                     /translation="MRDSEISSLSACNIHAIGDDCGMIEWLALIQESRRNRFLFFFLC
                     LVIETGVALIHTSLPYYANLILLYAFPIIGASWILGMRYGLTLALFSTVIWWVTQTSN
                     RPEEVAETVHIWNACSRAIIFFGIVLLISFLKEALTARERTLARLLARRSFLASVSHE
                     MREPLKRILAGSEILSTTASDPAAVIAVQAATQELMELVDAILELARLESGAVSFRAD
                     FNPVIVLREVVEHLTPVARQRNQMFSVEIDGNVPPVVRADQNCLRYSITRLLRDASEF
                     SESGAIIINLGYSREDQLLRFTVRDSGQANADQRNTSVRDGLGMHVAREMARLNGGHL
                     WQDENKTSEAIHHFTMIVDAV"
     gene            3126..>3763
                     /locus_tag="PKK76_14210"
     CDS             3126..>3763
                     /locus_tag="PKK76_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69683.1"
                     /translation="MDDPGRLTLAERYRQGIESLIHFDFERREEDLKWMLAESKKPVK
                     GISWLKEKYYELLRKCEEHPVPFQLIYDLETIASRLFPRAARKFSFIKDPIPEYYRAR
                     LKIYEPELREIADSEGHIRGADLLGVLGKMGARDGRSMVEMFLARLKDGKSHSSLASK
                     LEPGALEQLEVSLQRIPPNVPPEDLPALMQVAAQSSVSPIENEKLESHEAID"
BASE COUNT          989 a          970 c          951 g          853 t
ORIGIN      
        1 gagtgcgcct gtccggatgc ggtattagga tttcaacgga cagaccagcc ccgggaccat
       61 ccgaaaaggc gctcacgtat ccgccgagaa aacgaacaaa gtactttgtc atcgcaagtc
      121 caaggcccag tccgtcagga tcgccggtta tgccatgttc aaacattttc tccagcatgg
      181 cttgcgaaac accgctgccg gtatcatcga tcgtcaagtg aagaccggca gggctctctg
      241 ctgcgtaaac ggatattgat ccaaattgcg tgaacttgac agagtttgca atgaggttga
      301 gcaagatgtg ctgcaatctg cgcttatccg tcgggaactc cagctccgaa gaaacactga
      361 tgtccagcga aaccccttcg ggaatgaggg gctgaaaaag ggcagcaata gagtcaaata
      421 cctcacggag acgtaccggc gcgatttcga tttcgttctc atttccgttc gcgtagtcga
      481 ggagctcatg aatgaaggca ctcaagaatg aagcatggct ttcaatataa ttcaagtcac
      541 gcaagacgtg cgctgtttgt gcatccgctt tgatgacatc gaggcgagcg atgatcgcgt
      601 ttaagggcgt tcgcatatca tgactcacat aaccgagaaa gcgcgttttc gcctcgcttg
      661 cctcctcggc tcgctggtgt gcgaggcgaa gagcttcatt catcctgccg cctgtgtaga
      721 gcaaaagagc cacaactgcc atcaaagcaa tgcccaggga acgattcgca aagaccactg
      781 caacgggaac gcttgattcc ggagacatca tcgctccgcc aagcatcagc actgagaata
      841 tccagacttc gatggccagt aacttgccat gtggtcgcga aacgaagagg gctgggatat
      901 accagagcca tactgcgaat cctcgcggaa ccataaggtc cagggaaaaa atgacagctg
      961 ccagaacaaa aggcgcgatg tatctcaggg ccaccgaacg ctccccagta ttcctctgcg
     1021 ggcttcgtcg ccgtgtgttt cccgtgcatc gtcccaatat ccgccggaaa gatgaatccg
     1081 ggggcccgga acacgtagtc tccgcgataa aagatccagc gctttctcta gagcatagat
     1141 agaacgtgga tcatagccga actcgcttgc cacctgaaac tcaacagaag caagatcggt
     1201 cccaagatga tttcgcaaat ccgccagcat gtcaccgaag gctttttcaa aatcagatcc
     1261 cggtgtgcct tctagcgaaa tactcactcc tgccgatccg agactcttgt agtgaatctg
     1321 caaaggaaag cgttggagag gtacaagtcc cggcgttgcc ggtaattttc gagcaaggcg
     1381 caggaccagc tcagatggct tcaccggttt ggtaatatag tcgtctactt tcagttcacg
     1441 agcggacaga acatcctcac gttgatttga agcggtcatt acaagcacag gcttcccagt
     1501 tagaaacgct gtggcggatt ctatgaaggc caatccgcca acaattggca tccgtagatc
     1561 ggtgataacg gcatcatagg ggcgggaccg tacccagatc aaagccactc gtccgtctgt
     1621 cgccgaatcg agcagaatgt taaacgctgc aaacacgcgc attaccgatt cagcgaagcc
     1681 cgggtcatcc tctactagaa ggattcgata gaagggaatc atcttccttt tgagccgaaa
     1741 cctttggatc gacccatcct ccgacgtcaa aaatctagcg aacggaggaa catgcaatgt
     1801 agaatgtctt tgcctgggaa aaaataacgt tatgcgctca atgaaatgag attcgaattg
     1861 actttataga cgatagctgc cgaatttcga tcaaatactt tccggattca caattccgaa
     1921 caaaaaagag caggacctac tgcgaaggtc agatgcgtga ttcggaaata tcttcccttt
     1981 ccgcttgcaa catccacgca atcggtgatg attgtggtat gatagaatgg ttggcgctca
     2041 tccaggaatc tcgcaggaat cgtttcttat tcttttttct ttgtctcgtt atagaaaccg
     2101 gagttgcatt gattcacacg tccctacctt actacgccaa tctcatcctt ctctacgctt
     2161 ttccaatcat cggcgcttcg tggattcttg gcatgcggta cggtctaacg ctcgctcttt
     2221 tctcaacggt gatctggtgg gtgacacaga ccagcaatcg cccggaagag gtagcagaga
     2281 ctgtccacat ctggaacgca tgctctcgcg cgatcatctt cttcggcatt gttctgctga
     2341 tttcatttct aaaggaagca ctgacggccc gggagcgaac actggcgcgc ctacttgcgc
     2401 gaagatcgtt tcttgcatcg gtctctcatg agatgcgcga gccattgaaa cgaatcctcg
     2461 ctggaagtga aatactttcg accactgcaa gcgacccggc agcagttatc gctgtgcaag
     2521 cagccacgca agaactgatg gagctggtgg atgcgattct agaactcgct agacttgaga
     2581 gcggtgcagt gtcatttcgc gcagacttta atccagtaat tgttcttcgc gaagtagtag
     2641 agcatctgac acccgttgcc cggcagagaa atcaaatgtt ttctgtagag atagacggaa
     2701 acgtgccgcc agtggtgcga gctgatcaga actgcctcag gtattccata accaggctct
     2761 tacgcgacgc gtctgagttt tccgaatcgg gtgccatcat catcaacctc ggctactcca
     2821 gggaagatca gttgctgcgt ttcacggttc gagattcggg tcaagcaaac gcagatcaga
     2881 ggaatacctc tgttcgagat ggtctgggaa tgcatgtggc acgcgaaatg gcccgtctca
     2941 acggcggaca tctctggcag gacgaaaata agacatccga agcgattcat catttcacaa
     3001 tgattgtgga cgccgtatga ttgcatgaca tgaatcatat ccggctgggg gcatattcca
     3061 ctggaaatcg ccgctttttt gttgcgcgcg cgaggaagcc cacgacttgc tgaagaccgt
     3121 acgcaatgga cgatccaggc agacttacac tcgcagaacg ttaccgccag ggcatcgaat
     3181 cgctcatcca cttcgatttc gaaagacgcg aagaggatct aaaatggatg cttgcagaat
     3241 ccaagaagcc agtcaaaggc atttcgtggc tcaaggaaaa gtattacgag ctactgcgaa
     3301 agtgtgagga acatccagtt ccctttcaac tcatctatga tctcgaaacg attgcatcac
     3361 gcctgtttcc cagagccgca agaaaatttt catttatcaa agatccaatc ccggaatact
     3421 acagggcacg cttgaagatc tacgaacctg aactcagaga gatcgcagac tcagaaggac
     3481 acatccgcgg cgcagacctg cttggagtgc tcggaaaaat gggggccaga gacggccgga
     3541 gtatggtaga aatgtttttg gccagattga aagatgggaa gagccattca tctctcgcaa
     3601 gcaaactgga accaggagct ctcgaacagc ttgaggtctc gctgcagaga attccgccta
     3661 acgttcctcc ggaagatctt ccggcactca tgcaggtggc ggcccagtct tcagtgtctc
     3721 ctatcgaaaa tgagaagctc gaatcgcacg aagcaatcga tga
//
LOCUS       DAORFF010000393         3754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15043_length_3754_cov_3.725602, whole genome shotgun sequence.
ACCESSION   DAORFF010000393 DAORFF010000000
VERSION     DAORFF010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15043_length_3754_cov_3.725602"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="PKK76_14215"
     CDS             complement(<1..1231)
                     /locus_tag="PKK76_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69684.1"
                     /translation="MARVLLAASLTFFTTGLFSKEPIFPPGLKESEFKRMQEFYRALD
                     AGLVPTLDAVAEETKAEPPTRNLTDADEKSIQEIFPRIFFVDAASRGSLDIQAPVFVI
                     PNGNLAQITSSWNRVLLHGKNILRKPTADEIEEDTTFRRVDQVDAGNFRTIIRTSPHD
                     SGPVTAIGQVVLELPLTFHTATFDCEKPSAAKVGVYEFQLRRCTNDFVEFIQKTPEAH
                     PLEPTIRIYGASRDPLVVVQSSVDALFPGGKGILDVDFKSLPDRIEAKRYRFLVKGKA
                     KRIVLSYNDRVLKKTFSVTALSEPDYQNPKSSPNNRFVKVSPVASQMISESELRSSLK
                     VAAERNSAYFGFNTPRLILEAPAFSGLKYAKVEWKDVQLIDPKGQPVAFSLSDSACDF
                     EKHSCTVSLEDKTGDGFP"
     gene            complement(1237..1947)
                     /locus_tag="PKK76_14220"
     CDS             complement(1237..1947)
                     /locus_tag="PKK76_14220"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNL69685.1"
                     /translation="MKKLIAILLTASSFLALHAEEKALALVVQTAGKSDLVRDGKTLG
                     VKANDLVRKGDLLRTSKDASMSVQLSTGAIFRMGPDTEVALQDLVRDGTGFHVKLDVK
                     KGQLASKLDQLSKKDTYQVQTPTAVAGVRGTSFIVEISSAVQSAEVLVSDGAVAVTDN
                     KTGTEQVCPSGSKIVADGKAMIQSLVDEHEKEKFRIFADMKKFQRENFEQLIEQQKRN
                     QEMMRQQREQMNMDSVRP"
     gene            2032..>3754
                     /gene="acs"
                     /locus_tag="PKK76_14225"
     CDS             2032..>3754
                     /gene="acs"
                     /locus_tag="PKK76_14225"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNL69686.1"
                     /translation="MTMERIVQPFADFAAQANVKKSEYEALYKESVENPEAFWSRMAD
                     RLTWFKKWDKVLEHDFANAKSKWFTGGKLNVSYNCLDRHLSSTLKNKAAIIWEGDNPE
                     ESKTFTYQELHREVSRVGNVLRKRGVKKGDRVLIYMPMIPELAISALACTRIGAVHSI
                     VFGGFSPESLVGRIEDCKPVMIITSDAGYRGGKTIDMKRNIDAALEKSAQKVKDVIVV
                     RRVGQEQFYKWYEDRDHWWHDLLRDPSITAVCEPEQMDAEDPMFILYTSGSTGKPKGV
                     LHTTAGYLLGASLSHFYVFDHKPEDTYWCTADIGWVTGHSYILYGPLSNGATSIMFEG
                     VPQYPDWGRFWDVVDKYQVNVFYTAPTAIRALAKEGPAHVEKRSLKSLRLLGSVGEPI
                     NPEAWEWYFKHVGKEKCPIVDTYWQTETGSIIISPLPVFALKPGSATLPFFGIRPVLL
                     DSDGKKIEGEGSGNLAIEFPWPSIMRGVYGDPDRFHQTYFTQFKGYYFTGDGARRDKD
                     GYYWVTGRVDDVLNVSGHRIGSAEVEHAIVEHRAVAESAVVGYPHDIKGQGIYAYVTL
                     KNGIEASE"
BASE COUNT          967 a          926 c          940 g          921 t
ORIGIN      
        1 cggggaaccc atctcctgtt ttgtcttcca gagaaactgt gcacgaatgt ttttcaaagt
       61 cgcaggcaga gtcagagagg gagaaagcaa caggctgtcc ctttggatcg atcaattgaa
      121 cgtctttcca ttctaccttc gcatatttga gtccactgaa tgcaggagct tccaaaatca
      181 gtcggggagt attgaagcca aaatacgcgg aattccgctc ggccgcaacc ttcaaagacg
      241 atcgcagctc gctttcagaa atcatctgag acgcaacagg agacaccttc acaaaacgat
      301 tgttaggcga tgattttgga ttctggtagt ccggctcgga aagagcagta acagaaaaag
      361 tcttttttag aactcgatca ttgtaggaga gaacgattcg ctttgctttc cctttgacca
      421 gaaatcgata gcgctttgcc tcaatgcgat ccggcaacga tttgaaatcc acgtccagaa
      481 tcccttttcc tccagggaaa agtgcgtcta cagaagactg caccacaaca agggggtcgc
      541 gcgaagcccc gtaaatgcgg atggtgggtt cgagaggatg ggcctcagga gttttctgaa
      601 tgaattcaac aaagtcgttg gtgcatcttc tcaattggaa ttcatacact cctacttttg
      661 ctgcggatgg tttttcgcag tcgaacgtgg ctgtatgaaa ggtcagcggc aattccagta
      721 ctacctgacc gattgcggta accggcccgg aatcatgagg cgaagtgcga atgatagttc
      781 taaaatttcc agcatccacc tgatccacgc gacgaaacgt tgtatcttcc tctatctcat
      841 ccgctgttgg tttgcgaaga atattcttgc catgaaggag cacgcgattc cacgaactcg
      901 taatttgagc caggtttccg tttgggatta caaaaacggg agcttgaata tccagagatc
      961 cgcgcgatgc ggcatccacg aagaagattc tgggaaatat ttcctgaatc gatttctcat
     1021 ctgcatccgt caggtttctt gtgggaggtt ctgcttttgt ttcttccgcg acagcatcga
     1081 gggtgggtac aagccctgca tccagcgcgc ggtagaactc ctgcattctc ttgaactcgg
     1141 actctttgag tccaggaggg aaaatgggct ctttggaaaa gagcccggtt gtaaaaaatg
     1201 ttagactggc tgcgagtaaa actcgggcca atctcctcat gggcgaaccg agtccatgtt
     1261 catctgctca cgctgctgac gcatcatttc ctgattgcgc ttctgctgtt cgatgagctg
     1321 ttcaaaattt tcgcgctgaa actttttcat atctgcgaag attctgaatt tctctttctc
     1381 atgctcgtcc acgagactct ggatcattgc ttttccatcc gctacaatct tgcttccgct
     1441 gggacaaact tgctctgtgc ccgtcttgtt gtctgtgacg gcaacggcgc catcacttac
     1501 cagaacctct gcactctgca ctgcagatga aatttcaact atgaaagaag tgcctcgaac
     1561 accggccaca gcagtgggag tctgcacctg gtaagtgtct ttcttgctca actgatcgag
     1621 cttggatgca agctggcctt tcttcacatc cagctttaca tgaaatcctg tgccatcgcg
     1681 cacgagatcc tggagggcca cttctgtgtc cggccccatg cgaaagatcg cgcctgtaga
     1741 aagctgcacg gacatgcttg catctttgga tgtgcgaaga aggtcccctt tacgaaccag
     1801 atcgttggct tttacgccca gagttttgcc gtctcgcacc aggtctgatt tgcctgccgt
     1861 ttgaacgaca agagcgagtg ctttttcttc cgcgtgcaga gcaaggaagc tcgaagcggt
     1921 caataggatt gcaattagtt tcttcacgag atacctctta ggaaggacgg ggcagactgt
     1981 cgaatggttc gatttttctt gacccaatcc gcctcgcaat cgaaatccag cattactatg
     2041 gaaagaatcg ttcagccttt cgcggatttt gcggcccagg caaatgttaa gaaatcagaa
     2101 tatgaagctc tatacaagga atctgtagaa aacccggaag cattctggtc gcgcatggcg
     2161 gatcgtctga cctggttcaa gaaatgggat aaagtcctgg aacatgattt cgcaaacgcg
     2221 aaaagcaaat ggttcacggg cgggaagctg aacgtttctt acaactgtct ggacagacac
     2281 ttgagttcga ctctcaagaa caaagccgcc atcatctggg aaggggacaa tccggaagaa
     2341 tccaaaacgt ttacgtacca ggaacttcat cgcgaagtca gccgcgtagg aaacgtattg
     2401 agaaaacgcg gagtaaagaa aggagatcga gtcttaatct acatgcccat gattccagag
     2461 ctggccatca gcgccctggc atgcacgcgg atcggtgcag ttcactccat tgtattcggc
     2521 ggcttttccc ctgagtcgct cgttggcaga atcgaagatt gcaaaccagt catgatcatc
     2581 acttcagacg ctggctaccg cggtggaaag accattgaca tgaagcgcaa catagacgcg
     2641 gcactcgaaa agtcggccca gaaagtgaaa gatgtaatcg ttgttaggcg agtgggacaa
     2701 gaacaattct acaaatggta tgaagatcgc gaccactggt ggcatgacct tcttcgtgac
     2761 ccttcgatca ctgcagtttg cgaaccggag caaatggacg cagaagatcc aatgttcatc
     2821 ctgtacactt ccggctccac aggaaaacca aaaggagtct tgcataccac tgcgggctac
     2881 ttgcttggcg caagcctcag ccatttctac gtgttcgatc acaaaccaga agatacatac
     2941 tggtgcacag cagacattgg atgggtcacc ggacatagct acattctata cggaccgctt
     3001 tcaaatggtg ccacttccat catgtttgaa ggtgtaccac aatatccaga ctggggtcga
     3061 ttctgggacg tagtagataa ataccaggtg aatgttttct acactgcgcc cactgccatt
     3121 cgcgctcttg cgaaagaagg gccggcccat gtagaaaaaa gatctttgaa atcactgcga
     3181 ctcctcggga gcgtcggcga acccatcaat ccggaagcgt gggagtggta tttcaagcac
     3241 gtcggaaaag aaaaatgtcc gatcgtggat acctactggc aaactgaaac tggatcgatc
     3301 atcatttctc ctctccccgt cttcgcactt aaacccggaa gtgctacgct cccgtttttc
     3361 ggaattcgtc cagtgctcct ggacagcgat ggaaagaaga tagaaggaga aggctcagga
     3421 aacctggcga tcgaatttcc gtggccttcc atcatgcgag gagtctacgg agatccagat
     3481 cgttttcacc agacatactt cactcaattc aaaggatatt atttcacggg agatggagcg
     3541 cgtcgcgaca aagacggcta ctactgggtc actggccgcg tggatgatgt attgaatgtg
     3601 tccggccatc gcattggcag tgcggaagtg gaacatgcca tcgtagagca ccgcgccgtt
     3661 gcagaatcag ctgtggtggg ttatccacac gacatcaaag gtcagggcat ctacgcgtat
     3721 gtgactctca agaatggaat tgaagcatca gagg
//
LOCUS       DAORFF010000394         3746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15092_length_3746_cov_3.372799, whole genome shotgun sequence.
ACCESSION   DAORFF010000394 DAORFF010000000
VERSION     DAORFF010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15092_length_3746_cov_3.372799"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="PKK76_14230"
     CDS             <1..1140
                     /locus_tag="PKK76_14230"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNL69687.1"
                     /translation="LKSSSNQTRLVAVSALGEMNARSASGEILAMAMQNQDREVARTY
                     AMVLGKLGAVQSESYLLSILRSSKDDPASAAAINALGSIKSVQAIPDLVKVMGSSYAK
                     GRENAVPALIAIGSPLAIPGCFLLLENDSSEIRYAASEVIYSIPSPSTAPQALALLKK
                     KSRSAGPASMILGHIHYKAARETIEQVLQDKSMSDRENIAKSLGWMGESASSTVLISV
                     LKEKDGEGRYGAAWSLGVLRTPDGLGPLLEAADSGDRKLRQVAIEAIGSYGSSDSLPV
                     LSKLLKDSVLSIYAVDAISNIKGEEARKLLTDHAKNQTGGTRKAAIMALGRRKEEASV
                     PFLIQMLREDGTGEIQQSCMNALTEITGERFRTRGNWFDWFEKRQ"
     gene            1187..1546
                     /locus_tag="PKK76_14235"
     CDS             1187..1546
                     /locus_tag="PKK76_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL69688.1"
                     /translation="MPSEFSITTAIEEQIPILHIAGEITSESEEELLGAYDRIPGERK
                     NRVILHFSETRYINSSGIAILIGVINRANEVKGRVEFAGLSPHFRKVMDIVGLTDFVK
                     VYDSLQDALQASSANQS"
     gene            1625..2485
                     /locus_tag="PKK76_14240"
     CDS             1625..2485
                     /locus_tag="PKK76_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69689.1"
                     /translation="MRSLCPGAAILALLILAYPSFASPVSRYVDSFYPSKPTGELRLN
                     YYYDRTGKWVKFSDWIPYRKQRIQPRFFEDFYQIYGLPPAYNTGEVKESIYYLAAALG
                     TQFRHPSRALCTIRTEEEYHKYRNLMYMQTNLLIMRMFLRLGSLYDKRHLYFHDLDVA
                     DDLSVSFRIAKAYYEQAKPFWELARKHATVASQYRFDLDLPGIESERYRIMTGETNFD
                     RIIERHIADVDAKIQATDTFLAREGGSPRPVKAAMQKDIESMYDSEFTPDPLGPPVLQ
                     PHWNDKDPEP"
     gene            2500..3342
                     /locus_tag="PKK76_14245"
     CDS             2500..3342
                     /locus_tag="PKK76_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69690.1"
                     /translation="MRRAAVIAIFFFRTLGAQTIADFEEEYRLMTEREGFRGNPVAIE
                     RDWAKAESVPDPFPETPYVSQMEREEAQLRRAYSFYESAREIASELRQQLDAKPGRSI
                     RDPLWWKELDESAAESRRQRALRQRFAIDLAERYHRVFESLQELDRTAFRQDKRVISL
                     KRMAYRQYIILQSAVGNYIPAIEVLDEYARLEGAQTEWPLHYYYYVCLAGVFRDLKRS
                     SAVREEVLRSVRLRKNIHWRRAVELKFTKDSAEFDEVSRRIRSDLRDPPRSPIRPYSS
                     GRSL"
     gene            complement(3323..>3746)
                     /locus_tag="PKK76_14250"
     CDS             complement(3323..>3746)
                     /locus_tag="PKK76_14250"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNL69691.1"
                     /translation="TMVYGPGAAMSSAGVRVWNTPVMEAFLSTGLFAFYMLWARYQPF
                     RPGLQTAIFLCYNGVARFLVEFLRINNAVWAVLPPPVYEGKPLPHHNFISSNPPAYYF
                     ENWHWYGPTQAQFTALALVALGVTWIVVRKLYVKENVR"
BASE COUNT          955 a          916 c          995 g          880 t
ORIGIN      
        1 ttgaaatcca gttcgaacca gacaagactc gtggccgttt ctgctctggg cgagatgaat
       61 gcgagaagtg cttccggtga aatcctcgcg atggcaatgc agaaccagga cagagaggtg
      121 gcgagaactt atgcgatggt tctgggtaaa ctcggtgcgg tgcaatcaga atcctatttg
      181 ctttccattc ttcgcagttc aaaagacgat cctgcatccg cggccgcgat caacgcgctc
      241 ggtagcatca agagcgtgca ggcgattcct gatctggtga aagtaatggg aagttcgtac
      301 gcgaagggca gagaaaacgc agtgcctgct ttgatcgcga taggttcccc gctggccatt
      361 cctggatgtt ttcttctttt agaaaatgac tcgagtgaaa tccgttatgc ggcatctgaa
      421 gtgatctatt cgattccatc cccgtctact gctccgcagg ctctggcgct cctcaaaaag
      481 aaatccagaa gcgccgggcc ggcaagtatg atcctcggac acattcacta caaagcggcg
      541 cgcgaaacca tcgaacaagt tcttcaggat aaatccatga gtgatcgcga gaacattgcg
      601 aagtcacttg gatggatggg agaatctgcc agttcgactg ttctcatttc tgttttgaaa
      661 gagaaagacg gggaagggcg atacggtgct gcgtggtccc tcggagtgct tcgcactcca
      721 gatggactgg gacctctcct cgaagcggct gattcagggg atcgcaaact cagacaagtt
      781 gcgatcgaag cgatcggttc gtatggatcg tctgattctc tccctgttct ttcgaagttg
      841 ctcaaagatt ctgtgctcag catttacgct gtagacgcga tttcgaatat aaaaggagaa
      901 gaagcgcgca agttattgac cgatcatgca aaaaatcaaa cgggcggcac gcgaaaagcg
      961 gccatcatgg ctctgggtag aagaaaggaa gaggcatctg tacctttctt gatacagatg
     1021 cttcgggaag acggaaccgg cgaaattcag caatcctgca tgaatgcact caccgaaatc
     1081 acgggggaaa ggttccgcac tcgaggcaac tggttcgact ggttcgaaaa aaggcagtag
     1141 aaaagaattg gcagaagcaa gggttatttg attccttaat taaagcatgc cttctgagtt
     1201 ttccatcaca actgcgattg aggaacagat tcccatcctt cacattgcag gggaaatcac
     1261 gtccgaatca gaagaagagc ttctgggcgc ctatgatcga atcccgggcg aacgaaagaa
     1321 ccgggtcatt ttgcacttct ctgagaccag atacatcaat tcttccggga tcgccattct
     1381 cataggggtt atcaatcgag ccaacgaggt caaaggcagg gtagagtttg caggcctgag
     1441 tccccatttt cgcaaggtga tggacattgt cggattgact gactttgtca aagtgtatga
     1501 ttctctgcag gatgcgctgc aggccagttc cgccaaccag agctaacggc gcccattcct
     1561 tccagacgcg ctcgttcgtg agcattgcct aacgcttttg tccagatcgg ccgattctcc
     1621 gtttatgcgg tccttatgcc ccggagcggc aattcttgct ttgttgatcc tggcctatcc
     1681 ttcttttgcc tctcccgtgt cgcgctacgt ggactctttt tatccctcca aaccaacagg
     1741 agagctgcgc ctgaactact actatgaccg gacgggcaaa tgggtaaaat tttcagactg
     1801 gattccttat agaaagcaaa ggatccagcc gcgattcttt gaagactttt accagatcta
     1861 cggcctgcct ccggcttaca atacggggga agtgaaagag agtatttatt atctggcagc
     1921 cgccctgggc actcagtttc gtcatccatc gagagccctc tgtaccatcc gaacggaaga
     1981 agaataccac aagtacagaa accttatgta tatgcagacg aatctgctga tcatgcgaat
     2041 gtttcttcgt ctgggttctc tctatgacaa gaggcatttg tattttcatg acctcgacgt
     2101 tgcggatgat ctaagtgttt cttttcgaat tgcaaaagca tactacgagc aggcgaaacc
     2161 attctgggag ctcgcgcgaa agcacgcgac tgtggccagc cagtacaggt ttgatctaga
     2221 ccttcctgga attgagtcag agcgctatcg catcatgaca ggcgagacga attttgaccg
     2281 aatcattgag cgtcacatcg cagacgtgga tgccaaaatt caggcaacag atacattcct
     2341 ggcccgcgaa ggaggatctc cgcgcccggt caaggcagcc atgcagaaag acatcgaatc
     2401 catgtatgat tctgagttca ctccggatcc gctcggacca cctgtgcttc agccgcattg
     2461 gaatgacaaa gatcccgagc catgaaaaga atctaaatca tgagacgcgc ggcagtcatt
     2521 gcgattttct tttttcggac gttaggcgct caaacgattg ctgatttcga agaagagtac
     2581 aggttgatga cagagcgcga agggtttcgc ggaaatccag tcgccattga gcgcgactgg
     2641 gccaaggcgg agagtgtacc cgatccattt ccggaaactc cctacgtttc gcagatggag
     2701 agggaggaag cacagcttcg tcgggcatat agtttttatg aaagcgcgcg tgagattgcc
     2761 tcagaattga ggcagcaact agatgcaaag cctggccgaa gcatccgaga tccgctgtgg
     2821 tggaaagaac tggacgaaag tgctgctgaa agtcgcaggc agagagcact ccggcagaga
     2881 ttcgccattg atctagcgga aagatatcac agagttttcg aatccttgca ggagttggat
     2941 cgcaccgcat tcagacagga caaacgagtc atctctctca agagaatggc ttatagacaa
     3001 tacatcatac tccagtctgc agttggtaac tacattcccg ccattgaagt gctcgatgag
     3061 tatgcgcgtc tggaaggagc acaaacagag tggcctttgc actattacta ctatgtctgt
     3121 ctcgcaggtg tctttcgcga tctcaaaaga agttcagctg taagagagga agtgctcagg
     3181 tccgttcgtc ttcgcaagaa cattcactgg cgtcgcgccg tggaacttaa gtttacaaag
     3241 gattctgctg agtttgatga agtgagcagg cggatacgca gtgatttgcg tgatccgccc
     3301 agatctccga ttcgtcctta ttctagcgga cgttctcttt gacgtagagc ttgcgaacta
     3361 ctatccaggt cacacccagt gcgacgagcg ccagggccgt gaactgggcc tgagtcggcc
     3421 cataccagtg ccagttttca aagtaatacg cgggcgggtt ggagctgatg aagttgtgat
     3481 ggggcagtgg ctttccttcg taaaccggag gaggcagcac tgcccacaca gcattgttga
     3541 tgcgcagaaa ctcgaccagg aatcgcgcta ctccattgta gcacagaaag atagcagttt
     3601 gcagtccagg tctaaaaggc tggtatcgcg cccagagcat atagaaggca aacaggcctg
     3661 tggacaggaa cgcttccatc acaggagtgt tccagactct taccccggct gaactcatgg
     3721 cagccccggg gccgtagacc atggtc
//
LOCUS       DAORFF010000395         3740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15134_length_3740_cov_4.424966, whole genome shotgun sequence.
ACCESSION   DAORFF010000395 DAORFF010000000
VERSION     DAORFF010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15134_length_3740_cov_4.424966"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="PKK76_14255"
     CDS             <1..1136
                     /locus_tag="PKK76_14255"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HNL69692.1"
                     /translation="FRLPESDNPESFVLKLNDPHHVSASVFLHRLRISDVPYGSFIGK
                     DEREAAGGVDALSRVRETWQAQWTPATELALAERVVLGDSFERVCARRLRDSLSSAPR
                     TAEAAQILLEAVVADIDGIVSSALEVCNKFAANDGDVISLANACRTLSGLVSYGTSRK
                     SMEGAKAAIEALCSQTFSRVSLRIPDACKTDDDGAPSIQNAILAIHEIALAQKLVDKQ
                     LWFETARQLIRDYTIHPLCAGSIAGLLYVAREISDKEAQEIIEQRLTFRSQPAQAAGF
                     VAGFLEVNALIIVRDAGIVEKLNEFILELSADEFVNALPMLRRAFVQLGATERRYLIE
                     NILKLTGASPAESLAMLKEKDKEKLKTVSKEMGKIMKDLDDLL"
     gene            1133..2311
                     /locus_tag="PKK76_14260"
     CDS             1133..2311
                     /locus_tag="PKK76_14260"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNL69693.1"
                     /translation="MKQDAKEAILRWRLALGPGAEKTSSEFNLREGLGLPEERLSDLD
                     DALSFVYREKGAGGTGASAPYIPKWLGHLRDFFRQEVVAFVQKEAIEKRGLTQLLFEP
                     ETLPFLEKNVDLVSTLISARGLIPEKAKEIARQIVREVVEELRKKLESQTRTAILGAV
                     RRNNPSPLKVLRNLDWKKTIRKNLHGWNSETKRLVPEEIYFWSNQRRQHEWDVVLLVD
                     QSGSMATSVVYSSVMAAIFASLDVLKTKLVFFDTEIVDMTSYLTDPVEVLFSTQLGGG
                     TDINRAVAYAQANLIERPEKTLFILVTDLFEGGNQSELVMRMRQIVDGRSKTMVLLAL
                     SDRGQPSYDHGLATTLTEMGVHCFGCTPALLIGVVERIMKNQDVTEYVAAYQKAEKES
                     "
     gene            2308..>3740
                     /locus_tag="PKK76_14265"
     CDS             2308..>3740
                     /locus_tag="PKK76_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69694.1"
                     /translation="MSVRNIKPKELSDIIADPAELSKGIRIRDDGGLQHPARFENKLF
                     ADAKGSGASPYKVQIIYDEAENKFKGRCSCMAARSRPFCKHAVALLAAWSSAPEAFAV
                     SEGPPQGTPGETKKAKVKTSKVNTKNLMAEGVAQASTLVGELAISGLSGLSRQRLEQV
                     ESLGANLRESKLRRLSARLQTLAAQMSKASGIEKSAVTLAQLYSDAVLTVRKLEKHLA
                     GEELEAKYVEELIGKNWGKKDRKSQNGLDLLEYAYIHHTTADDFVIRESRFVDLQSGA
                     HYSEKQILPAFLAKKTQPKDSHGGVVLTNATVGCFPGFPPIRIDLEDPGNRVPADASA
                     FEKLLKVIVPDVETALGAFQERNSDVFAPDAMPIAMRVSAIFVRGRMQIVDAKGSAIF
                     LPAHEGIENALAFAIRGTTLHALFGDIILDGALPTLIPMAALVKSADMWELKPITSAF
                     KTASNTMDWHETARLAGVSASAVSVGE"
BASE COUNT         1032 a          883 c          987 g          838 t
ORIGIN      
        1 agttcagact cccggaaagc gacaatccag agagctttgt tctaaaactc aatgatcccc
       61 accatgtatc cgcatctgta tttctccaca ggcttcgaat cagcgatgtt ccgtacggat
      121 cgttcattgg aaaagatgaa agggaagcag cgggcggcgt agatgcattg tcgcgcgtca
      181 gagaaacgtg gcaggcacag tggactccgg caacagaact ggcccttgcg gagcgagttg
      241 tgctgggaga ttcgttcgaa cgcgtatgcg cgcggcgttt gcgcgattca ctttcatcgg
      301 cgcccaggac tgcggaggcg gcccagattt tgttagaagc agttgttgcg gatattgacg
      361 gaatcgtatc gagtgcgctg gaagtgtgca acaaattcgc ggctaacgac ggagatgtta
      421 tttcgctcgc caatgcttgt agaactctct ctggcctggt aagctatgga acgtcacgga
      481 aatccatgga aggagctaaa gcagctatcg aagctttgtg ctcgcagaca ttttcgcgcg
      541 taagcctcag aattccagat gcatgtaaga cagatgacga cggagcaccg tccatccaga
      601 acgcgatcct cgcaattcac gagattgctc tggcgcaaaa attggtggat aagcaactct
      661 ggtttgaaac agcgcgtcag ctgatccgcg actacacaat tcatccactc tgcgcgggtt
      721 cgatcgcagg cttactctac gttgccagag aaatttcaga caaagaagca caggagatca
      781 tagaacagcg cctaacgttt cggtcgcaac ctgcccaggc agccgggttt gtggcaggat
      841 tccttgaagt aaacgctctc atcattgttc gcgacgccgg aattgttgaa aaactaaatg
      901 aattcattct cgaattgtct gcagatgaat tcgtaaacgc actgcccatg ttgcgcagag
      961 catttgtcca actgggtgct acggaacgtc gctatctcat tgagaacatt ttgaaactaa
     1021 caggtgcttc tccggcggaa tcgcttgcca tgctgaagga aaaggacaaa gagaaactta
     1081 aaacggtcag caaagaaatg ggcaaaatca tgaaagacct ggatgatttg ctatgaagca
     1141 agacgcaaag gaagcgatac tcagatggcg actcgccctg gggccaggcg cagaaaaaac
     1201 atccagcgaa ttcaatctgc gagagggact cgggttgcca gaagagcgac tatcggatct
     1261 ggacgatgcg ctttcattcg tttatcgaga gaaaggggcc ggtggcacgg gcgcgtcggc
     1321 gccatatatt ccaaaatggt tgggccacct gcgcgatttt ttcaggcagg aagtagtggc
     1381 ttttgttcag aaagaagcga ttgaaaaacg cgggcttacg cagctactct ttgagccaga
     1441 aacactccca tttctggaaa agaacgttga ccttgtttcc actttgatca gcgcccgcgg
     1501 actgattccg gaaaaggcaa aagagatcgc gcgacaaatc gttcgagagg ttgtagagga
     1561 gctgcgcaaa aaattggaat cgcagactcg cactgccata ctcggggcgg taagaagaaa
     1621 caatccaagt ccactaaagg tgctgcgaaa tctagactgg aagaagacga ttcgcaaaaa
     1681 tctacacgga tggaattcgg aaacaaagcg attggttccg gaagaaatct acttctggtc
     1741 caatcaacgc aggcagcatg aatgggatgt cgtgttgctc gtagaccaat caggatccat
     1801 ggcaacgagt gtggtctata gttctgtgat ggcggctata tttgcatccc ttgatgtctt
     1861 aaaaacgaag ctggtcttct ttgatacgga aatcgtggac atgacttcct atctgacgga
     1921 tcccgttgaa gttttattct caacacagct cggcggaggc acggacatca atcgagccgt
     1981 tgcctacgcg caggcgaatc ttatagaaag gccagaaaag acacttttca ttctcgtgac
     2041 tgatctattc gaaggcggca atcaatcaga actcgtaatg cgaatgagac agattgtaga
     2101 cggcagatcg aagactatgg tactcctcgc attgtcagac aggggacaac cttcctacga
     2161 ccacggactg gcaacaaccc taacggaaat gggtgttcat tgttttggat gcactccggc
     2221 tctactcatt ggagttgtgg agcgaatcat gaaaaatcag gacgtcacag agtatgtagc
     2281 tgcttatcag aaggcggaga aagaatcatg agcgttcgca acatcaaacc caaagagctt
     2341 tcggacatca ttgcagatcc agcagagctg agcaaaggaa ttcggattag agacgatggc
     2401 ggcctccagc atccggcccg ttttgagaac aaactatttg cagacgcaaa aggttccggc
     2461 gcgtcgcctt acaaagttca aatcatttat gatgaagcag aaaacaaatt caaaggacgc
     2521 tgttcatgca tggcagcacg atcgcgaccg ttctgcaaac atgcagttgc acttcttgcg
     2581 gcgtggtcct ctgctccgga ggcctttgct gtaagtgagg gaccgccaca gggtacgccc
     2641 ggcgaaacta aaaaggcaaa agtaaaaacc agcaaggtaa acacgaaaaa tctgatggcg
     2701 gaaggggttg cgcaggcatc cacactcgtt ggcgaacttg cgatcagtgg tctgtctgga
     2761 ttgtctcgcc agagattgga acaggtggag agccttggcg caaacctgcg cgagtcaaaa
     2821 cttcgtagac tttctgcacg cctgcaaacc ctcgcggcgc aaatgtctaa agcgtcaggc
     2881 atagagaaat ccgctgtgac cctggcgcaa ctctattcag atgcagttct cacggttcgc
     2941 aaattagaaa agcatctcgc gggagaagag ctagaagcaa aatacgtgga agagctaatc
     3001 ggaaagaact ggggaaagaa agatcgaaaa tcgcagaacg gactggacct cctggagtac
     3061 gcctacatcc atcatacgac tgcagatgat ttcgtaattc gagagagtcg ttttgttgat
     3121 cttcagagtg gcgcgcacta ctccgagaaa caaattctgc ctgcattcct cgcaaaaaaa
     3181 actcagccaa aagacagcca cggtggagtg gttttgacga atgcgacagt tggttgtttt
     3241 cctggctttc cacccattcg tattgatctt gaagatccag gaaacagagt gcctgcagat
     3301 gcttccgcct tcgaaaaact tctgaaagtc attgtgccgg atgtagaaac cgcgcttgga
     3361 gcctttcagg aaagaaactc ggacgtcttt gctcccgatg cgatgccgat tgcaatgcga
     3421 gtcagcgcga tttttgttcg aggtagaatg caaatcgtgg atgcaaaagg ctccgcaatc
     3481 ttcctacccg cgcacgaagg aatcgagaat gcactcgcct ttgcaattag aggaaccacc
     3541 ctccacgcac ttttcggtga tatcattcta gatggtgcgc ttcccacttt gattccaatg
     3601 gcagctcttg ttaagtcagc ggatatgtgg gaactcaagc cgatcacatc ggcgtttaaa
     3661 acagcgtcga acacaatgga ctggcatgag actgctcgcc ttgcaggtgt atcagcgtcc
     3721 gcagtttctg ttggagagat
//
LOCUS       DAORFF010000396         3737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15155_length_3737_cov_2.886746, whole genome shotgun sequence.
ACCESSION   DAORFF010000396 DAORFF010000000
VERSION     DAORFF010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15155_length_3737_cov_2.886746"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PKK76_14270"
     CDS             <1..1091
                     /locus_tag="PKK76_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016560329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HNL69695.1"
                     /translation="FVVRDGGKQYYTRAGIFSMDRDGFYVHPGTGLRVQGWNARQDRD
                     GNSYINSAAGVEDIRIPLYQKKPAKATSEVVFESNLNQAVQSVPADATEEQIQQFLSG
                     PQETRRGHVATANVYDEEGNKRELRLEFYKTGDNRWQVRANMENSSNLSVNVVGPGGQ
                     DTAVGGTNAFELGFASDGRVQSVSDGVDTQNSGKLTVNVSFRIPGNPAVQTIKLNMGE
                     AGAVKGVTQFASSFTTHAKEQDGYPMGYLESFSIDNTGSIVGTYTNGVKEPLAKVALA
                     TFTNPAGLTKEGETKFSESMNSGNANVGEAGIGGRGKMFAGVLEMSNVDLSDQFTDMI
                     VTQRGFQANSRTITTSDQMIQEILGLKR"
     gene            1268..>3737
                     /locus_tag="PKK76_14275"
     CDS             1268..>3737
                     /locus_tag="PKK76_14275"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNL69696.1"
                     /translation="MNPDLKIRFLRAMLHARISDEAEQALKRKGLVPFELSSEGHEAL
                     AAIAFAMGPDDWLHPHYRDRAIVLARGVSDREIFLDFFSRSGAPSAGRQMPVHYNSRA
                     LRIVTLSTPVATNALQAAGMALSLKERGIPEVVIASIGDASSREGEFYEALAMAAADQ
                     LPVVFVIEDNGYGISTPTAGRTFWKMKHGVTENSDGSWFHGCAVLRVDGLDPEAVYEA
                     TLSAIDQARSGAGPTILIANVERLKSHSSSDDQRIYRSADELEHLKDPVKLYMQRCLD
                     SGIVDQRAIAVMRQELHDQVNEAARQAIGEGEPDPGQEIDSVFAPLPLSLCNAVEKLP
                     RPLSKDEGGRTMAQCIELVLEQEMKGNDRIYLYGEDIEDPKGDVFGITRGLSGKFPGH
                     VRNSPLAEATIVGSSVGRALAGDLPVAAIQFIDFVGPALNQLFNEVITIYWRSAGQWN
                     CPILILSPYGAYAPGIGPWHSQTNEALFAHMPGLHVVIPSSPADAAGLLRFSLRCQRP
                     VLFLYPKRLLHTAENTVEIPPEDCMVPFGRASVLRRGKDVTLVTWGNCVSICLSAASV
                     AHAEGIECEIIDLRTIIPWDRDAVLKSVRNTGRLIVVHEDARTCGFGSEIVAEVVAEC
                     FDSLISPPTRVTKGDVHNPYNMRLQLAALPSVEQTAAAVRSICQSRSDRAIRKERMPE
                     SHVTMSAAENVVNILVPQQSPTDEDAHLICFRVQLGDDVTEGAVVAEMEANKGAFEIE
                     STHSGRVIALHGSPGSRMLIGEKLLTLEVSSHNTKSQTPGARGREHQEHAGKEQADGP
                     NSAIVKLSPAQIRVSRIARMSLREI"
BASE COUNT          922 a          922 c         1048 g          845 t
ORIGIN      
        1 tcttcgtagt tcgcgacggc ggaaagcagt actatactcg cgcaggtata ttcagcatgg
       61 atcgcgacgg cttctatgtt catcctggaa ctggacttcg tgttcagggc tggaacgcgc
      121 gtcaggatcg cgatggaaac agctatatca attccgcagc tggcgtagag gacattcgca
      181 ttccgctcta tcagaagaag ccagccaaag caaccagtga agtagttttc gaatctaacc
      241 tcaatcaggc agtacagtca gtgcctgcgg acgcaacaga agaacagatc cagcaattcc
      301 tgagtggacc tcaagagact cgtcgtggac acgtggccac tgcaaacgta tacgacgaag
      361 aaggaaacaa gcgcgaactt cgtcttgaat tctacaagac aggcgacaac cgctggcaag
      421 tgcgtgcgaa catggaaaac tcttccaacc tcagtgtaaa cgtagttggt ccgggtggcc
      481 aggacactgc agttggtgga acgaatgcat ttgaactcgg ctttgcctcc gatgggcgcg
      541 tgcagtccgt ctcagacgga gtggacacac agaattcagg caagctcaca gtgaacgtaa
      601 gctttcgcat tccaggaaac ccggctgttc agactatcaa actgaatatg ggcgaagcgg
      661 gcgcggtgaa aggcgtcact caatttgcga gttcttttac aacgcatgca aaagagcagg
      721 atgggtatcc aatgggatat cttgaatcct tcagcatcga caacaccgga agcattgttg
      781 gaacatacac aaacggcgta aaagagcctc tcgccaaagt agctctcgca acatttacta
      841 acccggccgg tctcacgaaa gaaggcgaaa ccaagttcag cgaatcgatg aacagtggta
      901 atgcgaacgt gggtgaagca ggaatcggtg gacgcggcaa gatgtttgcg ggtgttcttg
      961 aaatgtcaaa cgtggacctg tctgatcaat tcacagatat gattgtcacg cagagaggtt
     1021 tccaggccaa ctctcgaacc atcacaacga gtgatcagat gatccaggaa atcctgggac
     1081 tcaagcgcta ggcctgagtg actaaaaacc cgccattcag gcgggttttt tatgcgacca
     1141 gggcaggatt atgtcacatg gccgggcggc cggattatgt cacataatct ccaggcaccc
     1201 tgacaaaaga tcttgcctgc tccaggaaat catgcgaact attgcatgta atctctggag
     1261 gcggcgtatg aaccccgacc tgaagattcg gtttctgcgt gcaatgctcc acgctcgcat
     1321 cagcgatgag gcagagcagg ctttgaagag aaaaggcctg gttccttttg agctatcaag
     1381 cgaaggccac gaggctctag cggccattgc ttttgccatg ggacccgacg actggcttca
     1441 tcctcattat agggatcgcg cgattgtgct ggctcgaggt gtgtctgatc gcgagatctt
     1501 tctggatttt ttttctagat cgggcgctcc gagtgccggc cgccagatgc ctgtgcatta
     1561 caattctcgc gctcttcgca tcgtcacatt gtccacgccg gtggcgacca atgcactgca
     1621 ggctgctggc atggctctgt ctctcaaaga acgaggaata ccggaagtag tgatcgcatc
     1681 cattggagat gcgtccagtc gcgaaggaga gttttatgag gcactcgcca tggccgccgc
     1741 agatcagttg cccgtggtgt ttgtaatcga agacaatggg tatggtattt ccactccgac
     1801 agccggtaga actttctgga aaatgaaaca tggagtcaca gagaattcgg atggatcctg
     1861 gtttcatggt tgtgccgttt tgcgtgtgga tggactcgat ccagaagccg tctacgaagc
     1921 aacgttaagc gccatagatc aggcgagatc cggggccggt cctaccatct tgattgcgaa
     1981 tgtagagagg ctcaaaagtc attccagttc agacgatcag agaatctata gaagcgcgga
     2041 tgaactggaa catctcaagg acccggtgaa actctacatg cagcgttgtc tggacagcgg
     2101 cattgtggac cagcgtgcga tcgccgtgat gcgccaggag ttgcatgatc aggtaaacga
     2161 agcggccaga caggcgatcg gggaaggaga gcccgatcct ggccaggaaa tcgattctgt
     2221 atttgcgcca cttccccttt ctctctgcaa tgctgtagaa aagctcccgc gccctctttc
     2281 caaagacgaa gggggcagga ccatggcgca gtgcattgag cttgtgctcg aacaggagat
     2341 gaaaggaaat gatcgcatct atctgtatgg tgaagacatt gaggacccaa agggagatgt
     2401 gtttggaatt acgcgcggac tttccggaaa atttccaggc cacgtgcgaa attctcctct
     2461 cgcggaagcg acgatcgtag gaagttccgt tggcagggca ctcgcaggcg accttcctgt
     2521 ggccgccatt cagttcattg actttgtcgg gcctgcgctc aatcaattgt tcaacgaagt
     2581 catcactatt tactggagat cggcggggca atggaattgt ccgatcttga ttctttctcc
     2641 gtacggggcg tatgctccgg gcattggacc atggcacagc cagacaaacg aagcgctctt
     2701 cgcgcatatg cctggacttc atgtggttat tccttcgagc ccggcggatg cggccggttt
     2761 gttgcgattc tctcttcgtt gccagcgtcc tgttcttttt ctctatccta agaggctcct
     2821 tcatactgcg gaaaacactg tggaaattcc tccggaagat tgcatggttc catttggtcg
     2881 tgccagtgtg cttcgccgcg gaaaagatgt aacactggtt acgtggggaa actgcgtttc
     2941 tatttgtcta tctgctgcct ctgtggccca tgcggaagga atcgaatgcg aaatcattga
     3001 tctgcggacc atcatcccgt gggacagaga cgcggttttg aaaagcgtta gaaacaccgg
     3061 tcgcctgatc gttgtgcacg aagacgcgcg cacgtgtggt tttggatctg agattgtagc
     3121 agaggtagtt gctgagtgtt ttgattcttt gatcagccca ccgacgcgtg tgaccaaggg
     3181 agatgtgcac aatccataca acatgcgcct gcagctcgca gcacttccat cggttgagca
     3241 gacagcggcc gccgttagat ctatctgcca gtcgcggagt gaccgcgcta ttcgaaaaga
     3301 gcggatgccc gaatctcacg taacgatgtc tgccgcagaa aacgtcgtga atatcctggt
     3361 tccgcagcaa tctcccacgg acgaagatgc tcatctcatc tgctttcgcg tgcaactggg
     3421 agatgatgtg acggaaggcg ctgtggttgc ggaaatggaa gcaaacaaag gagcttttga
     3481 aatcgaatcc acgcacagtg ggagagtgat tgcacttcat ggtagtccag gaagcagaat
     3541 gttgattgga gaaaagcttc tgactctaga agtctcatca cacaatacga aatcgcagac
     3601 gccgggagcc agggggcggg aacaccagga acatgcgggc aaggaacagg cagacgggcc
     3661 aaacagtgcc atcgtgaagt tgtctcccgc gcagattcga gtctcgcgga tcgcgcgcat
     3721 gagcctgaga gagattc
//
LOCUS       DAORFF010000397         3714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15315_length_3714_cov_3.735993, whole genome shotgun sequence.
ACCESSION   DAORFF010000397 DAORFF010000000
VERSION     DAORFF010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15315_length_3714_cov_3.735993"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PKK76_14280"
     CDS             complement(<1..755)
                     /locus_tag="PKK76_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69697.1"
                     /translation="MKSAILAWILVCVCACSCLIVADIYYCADQSALIRDSLSILHGH
                     FVSSGPESRGGVHHPGSLYKLLIALLLAVSGESLRVFAFMHVIVLSSSAFFIIRTTRI
                     LSGSIWFSLATLILFLALPFSFWYAMIVWGPAANVTAMCASLYFAVEIAKGKTALTPL
                     LMICLSFLFQTHYGTIFPVVALGLTGLWNSRTDKKAIHRALIVGAVFTLPALIDFVRE
                     PQNLRMILASAQGTQSLSSVWEAMVELHTMNAS"
     gene            complement(757..1353)
                     /locus_tag="PKK76_14285"
     CDS             complement(757..1353)
                     /locus_tag="PKK76_14285"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNL69698.1"
                     /translation="MRTVLYLLLALLVLECKKPPVVEEYRFVSASSGLRLRKTPDLKA
                     DKIATIGNGAKVRVVEKGEPVEVDGIRSNWLQIDFLGQKGWAFGGYLTRPTKKPDPQW
                     EKFMGTWRGKNECKGEHTHLLIHPDFTYEGWLLDTAGAACAGHAVTGTWSMELSSICL
                     VDPTRACYEYGKGGLLLANQQAAPALREGAELLMQLKK"
     gene            complement(1365..2267)
                     /locus_tag="PKK76_14290"
     CDS             complement(1365..2267)
                     /locus_tag="PKK76_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNL69699.1"
                     /translation="MKFSRAYFNQLLSPRAPNVDDRRLVLAGLPVYIVAAMSVFLVYA
                     QARRLMFMDVPAFEKQLSGEKKDEKIYPVLVEQNYRPKPETDQYRALSDVSAEGKGFL
                     TLNPGFHALTPYDRLELSAASRGSQSASSDNSANAAEGQGGKQGGNTGSNAPGNIGDD
                     ANFRIPQNYRFRQDFALRYDGSSRLSLARKELAGFRYFQKMIRQIEENFSPPGLNYAY
                     RDNAGLVTSEPVIPQSVQVLFMLDPEGNVRDVRKVSSIGQKAVDDACVNVLKGQNFGV
                     PPPEIFAQGNIFGINFIFPPVVNY"
     gene            complement(2242..3267)
                     /gene="ruvB"
                     /locus_tag="PKK76_14295"
     CDS             complement(2242..3267)
                     /gene="ruvB"
                     /locus_tag="PKK76_14295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNL69700.1"
                     /translation="MPEANKDFDEFSHDEAPEEKALRPGRLDEFVGQPLLKDRLSVSI
                     RAALHRKEPLDHVLLSGPPGLGKTTLACIVAAEMGGRFHSTSAPALGRPGDLARLLTL
                     LEKDDVLFIDEIHRLTRQCEEILYPAMEDGFIDFIVGEGMAAQSVKVPLKPFTLVGAT
                     TRSGSLTAPLKSRFGIDLKLEFYDLPELTKIVKRSAGLLGVQISENAAAVIAERARMT
                     PRAANRLLRRIRDYATVENVNIIEPDFALRALGKLGIDSLGLTDLDRKLLTLVIERYH
                     GGPVGIRTLASLLDEEEHTIEEDHEPFMLRIGLLEKTPQGRVSTQKAAIHLGLKIVPN
                     EIQQSLF"
     gene            complement(3281..>3714)
                     /locus_tag="PKK76_14300"
     CDS             complement(3281..>3714)
                     /locus_tag="PKK76_14300"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNL69701.1"
                     /translation="VCGSKMLRESMINSCRSFIFSTAQPPCLAHLLSLVFREISGMNV
                     ERKHLSFLSQQLREGLHSMNLDFGKSTSHIVPVILGSEEDAMRWMNSLALRGIDARAI
                     RPPSVPEGSSRIRVNLHAGHSVTDVECLLKELYSLLPVGVR"
BASE COUNT          957 a          958 c          917 g          882 t
ORIGIN      
        1 cacgaagcat tcatcgtgtg caactcgacc attgcctccc atacagaaga tagactttgt
       61 gtcccctggg ccgacgctag aatcatgcga agattctggg gttcgcgaac aaaatcgatc
      121 agggcaggaa gagtgaacac agcacccacg atgagcgcac ggtggatcgc cttcttatcg
      181 gtacgcgaat tccaaagacc ggtcaagcca agcgcaacca ccggaaaaat cgtgccatag
      241 tgagtttgaa acagaaaact caggcaaatc ataagcagcg gagtaagggc agttttcccc
      301 ttagcaatct ccacggcgaa atacaaagat gcacacatcg ctgttacgtt cgccgccggc
      361 ccccatacaa tcatcgcgta ccagaaagaa aaaggaagcg caagaaacag aatcagtgtg
      421 gccagggaaa accagataga tccgctcaag atacgcgttg tgcgaattat aaaaaacgcg
      481 gaggaagaaa ggactatgac gtgcatgaag gcgaagacgc gaagggattc ccccgacaca
      541 gcgagcaaaa gggcgatcag tagcttatag agtgagcctg ggtgatgcac gccgccgcga
      601 ctttctggac ctgaagaaac aaaatgaccg tggagaatag acagactatc gcgaatcaga
      661 gcagactgat ccgcgcaata gtaaatatct gcaactatta agcaggagca tgcgcaaacg
      721 cagaccagta tccaggccag gattgcgctt ttcattttac tttttcaact gcatcagtag
      781 ttcggcgcct tctctcagag caggagccgc ctgttgattg gccagaagca ggccaccttt
      841 cccgtactca taacaagcgc gggttgggtc taccagacaa atggaactca actccatgct
      901 ccacgttcca gtcaccgcat gcccggcgca ggcagcgccc gccgtgtcga gcagccagcc
      961 ttcgtatgta aaatcaggat gaatgagcag atgggtatgt tcgcctttgc attcattttt
     1021 gccgcgccag gtgcccatga acttttccca ctgcggatcc ggtttcttag ttggtctggt
     1081 aaggtagcct ccgaaggccc atcctttctg tccaaggaaa tcaatctgca accagttgct
     1141 tcgaatcccg tccacctcca ccggttctcc tttttccacc acgcgaactt tcgctccgtt
     1201 gccgatggtt gcaattttgt ctgctttgag atctggagtt tttctcaagc gaagcccgga
     1261 ggatgcggaa acaaaacgat actcttccac aacgggaggc ttcttgcatt cgagaacaag
     1321 aagtgcgagt agcaaataga gaacagtgcg catgggcgca aagttcaata gttcactact
     1381 ggaggaaaga taaaattgat gccgaagatg tttccctgag cgaagatttc aggcggcggg
     1441 actccaaaat tttgtccctt cagcacgttg acgcacgcat cgtcgactgc cttttgtcca
     1501 atactcgaaa cctttcgcac atctcgcacg ttgccttctg gatccagcat aaacaaaacc
     1561 tgaactgatt ggggaatcac gggttcactt gtcacaagtc cggcgttgtc gcggtatgcg
     1621 tagttgagtc caggaggaga aaagttttct tctatctgac gaatcatctt ttgaaaatag
     1681 cgaaagcctg cgagctcctt tcgcgccaga gaaagccgcg aagatccatc gtatctgagt
     1741 gcgaagtcct ggcgaaatcg atagttctgc gggattctaa aattcgcatc atcgcctata
     1801 tttccgggcg cgttggatcc ggtgtttccg ccctgctttc ctccctgacc ttctgcagca
     1861 ttcgcactgt tatccgagga tgcggactgg cttccgcgcg aagcagcgct cagttccagg
     1921 cgatcgtacg gagtgagtgc gtggaatcca gggttgagtg taagaaatcc tttgccttcc
     1981 gctgacacat ctgatagcgc acggtattga tctgtttccg gctttggacg atagttctgt
     2041 tctactagaa ccggataaat tttctcatcc tttttttctc cgctcagctg cttctcgaaa
     2101 gcgggaacgt ccatgaacat caatcttctg gcctgcgcgt atactagaaa cacagacatg
     2161 gccgctacga tgtacacagg caagccggcc agcacaagtc tacgatcgtc tacgttggga
     2221 gcgcgtgggc tcagcaactg gttaaaataa gctctgctga atttcattcg gtacgatctt
     2281 gagtcccagg tgaatcgcgg ccttttgcgt actcactctc ccctgaggag ttttttccag
     2341 taaaccaatg cgaagcatga aaggctcgtg atcttcttcg atggtgtgct cttcttcgtc
     2401 cagtagactg gccagtgtgc gaatgcctac tggtccgccg tgatatcgct caatcacaag
     2461 agtcaaaagc ttgcgatcga gatctgtcag accaagagaa tcgattccaa gtttgcccag
     2521 tgcgcgaaga gcaaaatcag gttcaatgat attcacattt tccacggtgg cgtaatctcg
     2581 aatgcgacgc aaaagtcggt tggctgcccg gggagtcatt cgcgctcttt ctgcaatcac
     2641 tgccgctgca ttctcggaga tttgtactcc aagcaagcct gctgatcgtt ttacgatttt
     2701 cgtcagctcc ggaaggtcat aaaattcaag ctttagatcg attccgaacc tggatttcaa
     2761 aggagcggtc agtgatccgg aacgagtggt agcgcctacg agcgtaaagg gctttagggg
     2821 aaccttgacg ctttgagccg ccattccttc tccgacgatg aaatcaatga aaccatcttc
     2881 catcgcagga taaagaatct cttcgcactg tctggtgagc ctgtgtatct catctataaa
     2941 aagaacatca tctttctcaa gaagagtcag cagcctggcc agatctcctg gacgtccgag
     3001 cgcaggagcg cttgtagaat gaaatctccc gcccatctct gcggcaacaa tgcatgcgag
     3061 cgttgtcttg cctagaccgg gtggtccaga aagcaaaacg tgatcgagag gttccttcct
     3121 gtgaagagcg gcgcgaatag aaacagacag tctgtctttg agaagaggct gccctacaaa
     3181 ctcatcgagc cgaccaggcc ggagagcttt ttcttccggc gcctcatcat gcgaaaattc
     3241 gtcgaaatct ttgttagctt ccggcaccga catgatcgat ttatctaacc ccgactggca
     3301 gaagactgta aagctctttt aaaagacatt ccacatctgt aacgctgtgg cctgcgtgta
     3361 gattgacgcg aatgcgagat gaaccctctg gcacactcgg agggcgaatg gctctcgcat
     3421 ctattccacg gagtgcaagg gaattcatcc atctcattgc atcctcctcc gagcccagaa
     3481 tcacgggaac aatgtgcgat gtgctttttc caaaatctag attcatcgaa tgcagtcctt
     3541 ctcgcagttg ttgcgatagg aaagacaaat gtttcctttc tacattcatt ccagaaattt
     3601 cccggaaaac aagagaaaga agatgagcga ggcatggcgg ctgcgcggtt gagaatatga
     3661 aggatctaca agagttgatc atgctctccc tgagcatttt cgaaccacaa acaa
//
LOCUS       DAORFF010000398         3701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15404_length_3701_cov_2.843664, whole genome shotgun sequence.
ACCESSION   DAORFF010000398 DAORFF010000000
VERSION     DAORFF010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15404_length_3701_cov_2.843664"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PKK76_14305"
     CDS             complement(<1..927)
                     /locus_tag="PKK76_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL69702.1"
                     /translation="MQSSRNRRRLEVKKEWDSSLAGRELRVRTVVTIDCQYLYPLTAC
                     AYMICEKDRVAFVENNTAHAVPLLLSALAEHGYSPEQVEYAIITHVHLDHAGGSGLLM
                     EKCPNATLLAHPRAARHVIDPARLVASAKMVYGEEVFAKVYGEIQPVEESRVRVMQDG
                     ETISFGERSLTFLHTRGHANHHFCVYDSGSDGIFTGDTFGIAYPFMQTQGPHLFPSTT
                     PTEFDPVEARLSIDRILATQASKAFLTHFGEFTHLEEGARQLKSGLDEFEAILKEAKE
                     RTETGEALTSLVEEKVNAYFKRRAHFLPETQLN"
     gene            929..1180
                     /gene="purS"
                     /locus_tag="PKK76_14310"
     CDS             929..1180
                     /gene="purS"
                     /locus_tag="PKK76_14310"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNL69703.1"
                     /translation="MYLARIAIRLKPSIQDPQGLAIVHALHELGFSSTESVRMGKYAE
                     VKINAPDKAAAEKAAKDMCEKLLVNSVMEVYDLTVEESR"
     gene            1177..1842
                     /gene="purQ"
                     /locus_tag="PKK76_14315"
     CDS             1177..1842
                     /gene="purQ"
                     /locus_tag="PKK76_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNL69704.1"
                     /translation="MKAGVVTFPGSNCDQDMILVLRQFFGATVESLWHADQFQGSFDL
                     IVIPGGFSYGDYLRTGAIARFAPAMKSVAEHAKRGGVTIGICNGFQILCEAGLLPGAL
                     IRNVTLKHICKDVQLRAKVNPFTRGLTPNQIVTLPVSHGDGNYRADDDVIRSLFDHDQ
                     VAFEYVQNVNGAEKNIAGISDKSGRVLGMMPHPERAVDPATGGTDGKAVLESILNVCR
                     AHV"
     gene            1820..>3701
                     /gene="nadE"
                     /locus_tag="PKK76_14320"
     CDS             1820..>3701
                     /gene="nadE"
                     /locus_tag="PKK76_14320"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNL69705.1"
                     /translation="MSAELTSDVIRLSLVPLNQIPLDWKGNRARILSALEELKSPAPE
                     IVLFPELCITGYGCEDAFYSLDVAERALSSALEVAQKAHAILDTSVVVGLPVRYHERI
                     YNCAAILREGKIQCIIPKQNLAGDGIHYEPRWFTAWRGRPVEYELPDHSQVPMGPMVL
                     DQSGIRIAFEICEDCWVPNRPADKYTEASPDLILCPSASHFAFGKENIRRGIAVDSSR
                     SYACVYAMVNLLGNESGRSIYDGCAIVASLGNLLYEGTRFSFKDYTLHTVDVDIAAVR
                     SARSRLYSFKEQSELAEYAMLPLKIKRAKILSATPPGPPDSMSKELEFLHAVSLGLFD
                     YLRKSHSKGYVVSLSGGADSAACALLIERMLRLAERELGSLANQKLGLTPNAGVHDIL
                     HTIYQAAEQSSQTTRTAAREVAMALRADHHEESISDTVESYTTKIETILGRKLTWQTD
                     DLSLQNIQARVRSPLAWLFANTTGSILITTSNRSEGSVGYCTMDGDTSGGLAPIAGVD
                     KAYLKQWLLWMEKTGDEFGPVQALRYINEQEPTAELRAGQTDERDLMPYSLLDQIEAL
                     AVRDKKSPKEIYQILSGKSIPDLSRHIARFFRLWTQNQWKRERLAPSFHLDDRNIDPR
                     SWM"
BASE COUNT          967 a          946 c          901 g          887 t
ORIGIN      
        1 attcaactgt gtttccggca aaaagtgagc tcgccttttg aagtatgcat tcactttctc
       61 ttctacaaga gaagtgagtg cctcgcctgt ctctgttctt tcttttgcct cttttaggat
      121 cgcctcaaac tcatccagtc cgcttttcaa ctggcgcgcc ccttcctcca gatgagtgaa
      181 ttcaccaaaa tgagtcagaa acgccttcga tgcctgagtg gctagaattc gatcaatgct
      241 cagtcttgct tccactggat caaattcagt aggtgtggtg ctcgggaata agtgagggcc
      301 ctgagtctgc atgaaaggat atgcaatccc aaatgtatcc cctgtgaaaa tcccgtctga
      361 gccggaatca taaacgcaaa aatgatgatt tgcatgacca cgtgtgtgta aaaacgtcag
      421 actcctttcc ccaaaagaaa tggtttcccc atcctgcatc acgcgaacgc ggctttcttc
      481 tacgggctga atctccccgt acactttcgc aaatacttct tctccgtaga ccatctttgc
      541 actcgccacc agacgagcag gatcgatgac gtgtcgggct gcacgaggat gtgcgagcaa
      601 ggtagcattg ggacattttt ccatgagcag tccactgccg cctgcatggt ccagatgcac
      661 atgagtgatg atggcgtatt ctacttgctc gggactgtaa ccgtgttccg ccagagcaga
      721 caaaagcaag ggaacagcgt gcgcagtatt gttttctaca aacgcaactc tgtctttttc
      781 gcaaatcatg tatgcgcagg cagtgagtgg atatagatac tgacaatcaa tggttactac
      841 agttcgcacg cgcagttctc tccccgccag ggaagaatcc cactcttttt ttacttccag
      901 gcgccggcgg tttcgggaac tttgcattgt gtatctcgcc agaattgcca ttcgcttaaa
      961 gccctctatc caggatcctc agggactggc catcgttcat gctctgcatg aacttggatt
     1021 ttcctccaca gaaagtgtga gaatgggcaa gtatgcagaa gtaaagatca acgcacctga
     1081 taaagctgcg gcagaaaaag cagcgaaaga tatgtgcgaa aagctactcg taaattctgt
     1141 aatggaagta tacgatctca ctgtagagga aagcaggtga aagcaggagt cgttacattc
     1201 cctgggtcaa actgcgacca ggatatgatt ctggtcttgc gccaattctt tggagcaacc
     1261 gtagaaagtc tgtggcatgc agatcaattc cagggatcgt ttgatttgat cgtaattcct
     1321 ggcggtttct cttacggaga ttatcttcgc acaggggcca ttgcacgatt tgctcctgcc
     1381 atgaaatcgg tggcggagca tgcaaagcgc ggcggagtca ccattggaat ctgcaacggc
     1441 tttcaaattc tgtgcgaagc cgggctgctc cctggtgctc tcattcgcaa tgtgactctc
     1501 aagcatatct gtaaagatgt gcagcttcgc gcaaaagtaa atccattcac tcgcggcctg
     1561 actccaaacc agatcgtcac tcttccggta tcacacggag atggaaacta tcgcgcagat
     1621 gatgatgtca ttcggtctct gttcgaccat gatcaggttg cctttgagta cgtgcaaaac
     1681 gtaaacggag cagagaaaaa catagccggc atttcagaca aaagcggtcg cgtactcggg
     1741 atgatgccgc atccggagag agctgtggat cccgccaccg ggggcacgga cggtaaggca
     1801 gttctggagt ccatactcaa tgtctgcaga gctcacgtct gacgttatac gcctttcact
     1861 cgtaccactg aaccagatcc ccctggactg gaagggaaac cgcgcccgca ttctatctgc
     1921 actcgaagaa ctcaaatcac ccgcacctga aatcgttctt ttcccggaac tctgcatcac
     1981 cggttacgga tgcgaagacg cgttttattc tctggatgta gcagagagag ctctgtcctc
     2041 cgcgctcgaa gtggcgcaaa aagcccacgc tattttagat acaagtgtgg tagtgggttt
     2101 gccagtgcga taccacgaaa gaatttataa ttgcgcggcg attctgcgag aaggcaagat
     2161 tcaatgcatc attccaaaac aaaacctcgc gggagacgga attcattacg agccaagatg
     2221 gttcaccgca tggagaggtc ggcctgtaga atacgaactt ccggatcata gccaggttcc
     2281 catgggacca atggtcctcg atcaaagtgg aattcgaatc gcgtttgaaa tttgcgaaga
     2341 ctgctgggtt cccaatcgac cggcagataa atacacggaa gcctcccctg atctcattct
     2401 ttgtccttct gcatctcatt tcgcatttgg aaaagaaaac atccgccgcg gaattgcagt
     2461 cgattcatca agatcctatg cctgcgttta cgcgatggtg aatctgcttg gaaacgaatc
     2521 agggcgatcc atctacgatg gatgtgcgat tgtggcatct cttggaaatc ttctctatga
     2581 gggcacacga ttctcgttca aagactacac tctgcacaca gtggacgtgg acattgcagc
     2641 cgttcgatct gcaagaagca ggctctactc tttcaaagag caaagcgagc ttgcagaata
     2701 tgcaatgctt ccactgaaga taaaacgtgc gaagattctt tctgctactc cgcccggtcc
     2761 gccagattcc atgagcaagg aacttgaatt tctgcacgca gtttctctgg ggctattcga
     2821 ttatcttaga aagtcgcact cgaaaggcta cgtggtctct ctgtccggcg gagccgattc
     2881 tgcggcctgt gctcttctta tagaaagaat gcttcgcctg gcagagcgtg aacttggatc
     2941 tcttgccaat caaaagcttg gactcacgcc aaacgcaggc gtgcacgaca ttctacatac
     3001 aatctatcaa gcagcagaac aaagctcaca gaccaccaga actgcagcgc gtgaagtggc
     3061 catggctctt cgcgcagatc accatgaaga gagcatttca gacacagtag aaagctatac
     3121 gacaaagata gagacaatcc tcggaaggaa gctcacctgg cagacagatg atttgagcct
     3181 ccagaatatc caggcgcgtg ttcgctctcc tcttgcctgg ctcttcgcaa atacaactgg
     3241 aagcattctc attacaacaa gcaaccgcag cgaaggaagc gtgggctact gcaccatgga
     3301 cggagatacg tcaggcggcc ttgctcccat cgccggagtg gacaaagcat acttaaagca
     3361 atggcttctg tggatggaga agacgggaga cgaattcggg ccagtacagg ctcttcgcta
     3421 catcaacgag caggaaccaa ccgcagaact gagagcgggc cagaccgatg aaagagatct
     3481 catgccctat tctctgctcg atcagatcga agctctcgca gtgagagaca aaaaatcgcc
     3541 aaaggaaatc tatcagattc tttccgggaa atccatcccg gatctttcca ggcacattgc
     3601 ccgattcttt agactgtgga ctcaaaatca atggaagaga gagagactcg ctccttcctt
     3661 tcatctggat gacagaaaca tagatccgcg aagctggatg c
//
LOCUS       DAORFF010000399         3695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15451_length_3695_cov_3.722253, whole genome shotgun sequence.
ACCESSION   DAORFF010000399 DAORFF010000000
VERSION     DAORFF010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15451_length_3695_cov_3.722253"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PKK76_14325"
     CDS             <1..574
                     /locus_tag="PKK76_14325"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNL69706.1"
                     /translation="PALFWLFSRRAALELLLMIIATAYTNSILKWVFSRPRPYWISDS
                     VQALAEETTYGIPSGHAQNAVVVWFMLAVFAVRKWPNRKTAIYGVATFIVALISFARI
                     YLGVHFIEDIVAGWLFGSILLALYFKFHKPMEEWWRVSPARVGVVLLPVVLLWFAFKV
                     RTLSPGIEDLFPWRPSSAPRAVSWVSSLSF"
     gene            607..786
                     /locus_tag="PKK76_14330"
     CDS             607..786
                     /locus_tag="PKK76_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69707.1"
                     /translation="MRIVLLVVTLAIVLGLQVGLTKLFAPYTHMEMEFRFIRYFLVCI
                     VGFLLIPWSAKKLLS"
     gene            809..2053
                     /gene="ccrA"
                     /locus_tag="PKK76_14335"
     CDS             809..2053
                     /gene="ccrA"
                     /locus_tag="PKK76_14335"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004444708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HNL69708.1"
                     /translation="MAQEIVPVGTLPPLGEVPKKMHAQLVRPERYGEPRTAFQVEQID
                     VPDIAPDEVLVAVMAAGVNYNNVWAALGYPVDVIAARNKKGEPEKFHIGGSDASGIVY
                     KVGSDVKNVKVGDEVVVHCGIWDRNDPWVKAGKDPMFAPSQIIWGYESNWGSFAQFCK
                     VQDHQCLPKPPHLTWEEAAAYMLVAATAYRMLHHWSPNSVQKDDVVLIWGGAGGLGSM
                     AIQIAKAAGALPIAVISSDDKIEYCKKLGAAGTINRNNFKHWGALTTEINKGEVFGQW
                     TKSAREFGKAIWDIAGKGRNPQIVFEHPGESTLPTSVFVCETGGMVVICAGTTGFNAT
                     GDLRYLWMRQKRLQGSHFANDENCKGLNDLVMQKKVDPCLSKTYKFEETGACHQLMRE
                     NKHPHGNMSILVGAPSEGLGRK"
     gene            2112..3125
                     /locus_tag="PKK76_14340"
     CDS             2112..3125
                     /locus_tag="PKK76_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNL69709.1"
                     /translation="MKSFEIQSAFGIENLKISERPDPVPGHGEVLVRMRAASLNYRDF
                     LTVIGQYNPKYKLPLIPGSDAAGEVVSTGPGVSEYKTGDRVMSCFALDWISGTPGIKQ
                     VRNSSLGGPLDGTLTELRVFPEKSLVRIPDFMTYEEASTLPCAGVTAWSALVTEAVVP
                     PGSTIVVLGTGGVSIFALQIGKMLGARVIVTSSSNEKLELARKLGADEVINYREKPQW
                     SKEVRKLTSMEGADHIIEVGGAGTLEESLRAVKFFGQISLIGILAGSARDLNLLPILM
                     QNVRMQGILVGSRDSLASVARAYEIHKVRPVIDRTFSFDQTPAAIQYLADGKHFGKVC
                     ITI"
     gene            3198..>3695
                     /locus_tag="PKK76_14345"
     CDS             3198..>3695
                     /locus_tag="PKK76_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69710.1"
                     /translation="MRILIFLLLMPLSLVADTVRLRDGSVLTGKVTCQDPSSITLESK
                     DGVQSISKSEIAAFREDDSGTESYKRRLADARAASETRQKQIDQAIADRRQAVPTGFW
                     IGMGAGSGRIYFPQESILIHDRIEASLIPNGAQANAFPGFARRPANGEDANAGYQGDR
                     FYVEAD"
BASE COUNT          891 a          907 c          983 g          914 t
ORIGIN      
        1 gccggctctg ttctggctct tcagccgccg cgccgctctg gaactgctcc tgatgatcat
       61 cgctactgct tacacgaata gcattctcaa gtgggtgttc tctcgcccca ggccctactg
      121 gatctctgac agcgtccagg ctcttgcaga ggaaacaaca tatggaattc cgtccggaca
      181 tgctcagaat gctgtggttg tctggttcat gctggctgtg tttgccgtgc gaaaatggcc
      241 gaatcgtaaa acggccatct acggagtcgc aaccttcatc gtcgcgttga tcagtttcgc
      301 ccgaatttat ctcggggtgc attttataga ggacattgtt gcaggctggc tgtttggctc
      361 cattctcctg gctctttatt tcaaatttca taaaccgatg gaagaatggt ggcgagtctc
      421 tcctgctcga gtgggtgtcg tccttttgcc agtcgtctta ctgtggtttg cttttaaggt
      481 gcgtacactc agtcccggta tagaggacct ctttccctgg cgaccgtcat cagcacccag
      541 ggcagtctcc tgggtctcat cattgtcttt ttgattcctg tggtgagttt ccaacctcat
      601 ttgcgcatgc gaattgtgct tcttgtggtt actctggcga ttgtcctggg tttgcaggtg
      661 gggctgacga agttatttgc gccttacacc cacatggaaa tggaattcag attcattcgg
      721 tatttcctcg tttgcattgt tggttttctt ctgattccct ggtctgcaaa aaaattgctc
      781 tcctgagtga tccggtactt ttgggcgaat ggcacaagaa atcgtacccg ttggaacact
      841 ccctccgctt ggagaagttc ccaaaaaaat gcacgctcag ctcgtaagac cagagcgata
      901 cggagaacct cgcactgcgt ttcaggtaga gcagatcgat gttccggaca ttgctccgga
      961 tgaagtgctc gttgcagtga tggcagcagg tgtgaactac aacaacgttt gggccgctct
     1021 gggttatcca gtagatgtaa ttgccgcgcg aaacaagaaa ggagaacctg agaagttcca
     1081 catcggcggt tcagatgctt ctggaatcgt ttataaagtc ggaagcgatg tgaagaatgt
     1141 aaaagtggga gatgaagtgg tcgtccattg cggaatctgg gatcgcaacg atccgtgggt
     1201 gaaagcagga aaagatccaa tgtttgctcc atcgcaaatc atctggggtt atgaaagcaa
     1261 ctggggatcc ttcgctcaat tctgcaaagt gcaggatcat caatgtcttc caaaacctcc
     1321 tcatcttacc tgggaagaag ccgcagcata catgctggtt gctgccactg cgtatcgcat
     1381 gcttcaccac tggtctccca acagcgttca aaaagacgat gtggttttga tctggggcgg
     1441 tgcaggtgga cttggttcca tggccattca gattgcgaaa gcagcagggg ctcttcccat
     1501 tgcagtgatc agttcagacg acaagattga atactgtaag aaactgggtg cagccggcac
     1561 aatcaacaga aacaatttca agcactgggg cgctctgaca accgaaatca acaaaggcga
     1621 agtgttcggc cagtggacca aatcagcgcg cgaatttgga aaagcaatct gggacatcgc
     1681 aggaaagggc aggaatcctc agatcgtatt tgaacatcca ggagaatcca ctctaccaac
     1741 ttctgtattc gtatgcgaaa caggcggtat ggtagtcatc tgcgctggaa caactggctt
     1801 caatgcaaca ggggatcttc gttatctctg gatgcgtcag aagagattgc agggttctca
     1861 cttcgcaaac gacgaaaact gcaaaggact caatgacctg gtcatgcaga agaaagtgga
     1921 tccatgcctc tccaaaactt acaagtttga agaaacaggt gcatgccacc agttgatgcg
     1981 cgaaaacaag catcctcatg gcaacatgtc gattctagta ggcgctcctt ccgaaggcct
     2041 gggtcgcaag taaatagatt ccggcgtccg cagtcgcggg cgccgatgga tcgcctatca
     2101 ggagaatcgc aatgaagtct tttgaaattc agtccgcgtt tggaatagaa aatctgaaga
     2161 tttcggaaag accggatccg gttccaggtc acggagaagt gctcgttcgt atgcgcgccg
     2221 cttccctgaa ctacagggat tttctcacag tcattggtca gtacaatcca aaatacaaac
     2281 tccctttgat ccctggttca gatgctgcgg gtgaagtggt ctccaccggt cctggagttt
     2341 ctgaatacaa gacaggagac agagtgatgt cctgtttcgc tctggactgg atttccggca
     2401 ctccaggtat caaacaagtt cgcaattctt ctctgggcgg tccactggac ggaacactca
     2461 cagaacttag agtttttcct gaaaagagcc tggtgcgaat tcccgatttc atgacctacg
     2521 aagaagcatc cactcttccg tgtgcgggtg taaccgcatg gagcgctctg gtcacagaag
     2581 ctgtagttcc acctggcagc acgattgtgg ttctgggtac tggcggcgtt tcgatcttcg
     2641 ctttacagat cggcaaaatg ctcggcgcga gagtcattgt tacttcgagc tcgaatgaga
     2701 agctggagct tgcgcgaaaa ctgggggcag atgaagtcat caattaccgc gaaaagccgc
     2761 aatggtccaa agaagtgcgc aagctcacca gcatggaagg cgcggaccac atcattgaag
     2821 tgggcggggc cgggactctg gaagaatcgc ttcgcgcggt gaagtttttt ggacagatca
     2881 gcttgatcgg aattctagct ggatctgctc gcgatctaaa tctacttccc atcttgatgc
     2941 agaatgtgcg catgcaggga atactcgtgg gaagccgcga cagtctggct tctgtggccc
     3001 gcgcatacga aattcacaaa gtcaggccag tcatagatcg gactttttcg tttgaccaga
     3061 ctccggcggc catccagtat ctggcagatg gcaaacactt tggcaaagtc tgcatcacaa
     3121 tctgaagctc aaagcgcgcc acggttcgct gagaaacgcg gacacatcat cgaaaaagtc
     3181 gtctgatcgg ggttcatttg cgcatcctta tctttctatt gctgatgcct ctttcgctcg
     3241 tggcggacac cgtgcgcttg cgcgacggtt ctgtactcac cggtaaagtc acgtgccagg
     3301 atccatcgtc gatcactctt gagtcaaaag acggcgttca atcaatctcc aaatcagaaa
     3361 ttgcggcatt cagagaggat gactccggga ccgaatcata caagcgcagg cttgcggatg
     3421 cgcgcgctgc atcggaaacc agacaaaagc aaatcgatca ggcgatcgca gatcgcaggc
     3481 aagctgttcc cactggattc tggatcggaa tgggcgcggg ttcggggcga atttattttc
     3541 cgcaggaatc cattctcatc catgatcgca tcgaagccag tttgatccca aatggagcgc
     3601 aggccaatgc gtttccaggt tttgcgcgac gtcctgcaaa tggtgaggat gcaaatgcag
     3661 gctatcaggg agatcgcttc tacgtggaag cggat
//
LOCUS       DAORFF010000400         3694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15458_length_3694_cov_3.428140, whole genome shotgun sequence.
ACCESSION   DAORFF010000400 DAORFF010000000
VERSION     DAORFF010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15458_length_3694_cov_3.428140"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..216)
                     /locus_tag="PKK76_14350"
     CDS             complement(13..216)
                     /locus_tag="PKK76_14350"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL69711.1"
                     /translation="MSNNVYDRIFARFYDTFMEKPEKVELARRRRRLLRDLKGNILEI
                     GAGTGASFQWYGPAAHVLAVEPS"
     gene            complement(222..1130)
                     /locus_tag="PKK76_14355"
     CDS             complement(222..1130)
                     /locus_tag="PKK76_14355"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNL69712.1"
                     /translation="MKKTLTIAMVLFVAYATAAFYFSGEITTFKVKSKETVLAEDNLR
                     GYADVDLPRPEDVSVTGGQVELNGWLFVNRKNRRCGVILHHGHAANRNGMLKYAKLFW
                     PRGCHLLMFDARHHGQSAGTFATWGVRERDDLVIVADWFAKRTGLPHEKIGLFGASMG
                     AAIVLQAAPLTPDFAFVAADSPFRDLDSILKYRGSTLYGKAPILALYPAASWIAGLRG
                     NFRTEDASPLSAAPHIEIPVFMTHVKDDDAIPFTHSEEVFAAIGHNRKTLHLVDKGGH
                     CGLIKTNPTLYAAWMDDFLKKFAPEF"
     gene            complement(1127..2911)
                     /gene="lnt"
                     /locus_tag="PKK76_14360"
     CDS             complement(1127..2911)
                     /gene="lnt"
                     /locus_tag="PKK76_14360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNL69713.1"
                     /translation="MASPRSAGNTKKNEGLPLPGFFANPWIEVIAGCIVFGGLCTLSL
                     DPFLIPGIVWICSWPLFHFADRYQNHAWKLLGIGALGAMFLCLFSFYWMLHLFSTYGG
                     LNIALGLLIFIPYTILLNLKIPMFLVAFGFAKRFETKLPPMWLVAATLCVLTDYLAPQ
                     VFPWYWGNLAGKNRPLSQVAEFAGVYGISFLHFAGSYALYALFKTILHSIAQGQEKAS
                     EAVMSALKSGAWSDNRGKWAVPTLFAIVLVWGYVRLYQMANVEKQSPHVRVAMIQPNT
                     RLPRAGEGLIPRDEVRDILENRIPSLVGKAAAAGPLDMIVLPESAVPNNLTTQDNMVT
                     RIEGIYSPEFASMMQKLATKYKATVYANEVAADVKLDRVTGRPRPIEFNSSIVYSPDG
                     GRREYYHKRTLLAFGEYIPGAEFLDSSGLIALVPEAVRYSRFERGESAHNLTYYVKKE
                     GAQPAGTFLPLICYEVLIPEYVRSYFDDSNPGFIVNITQDGWYGKTVETYQHFGLGRI
                     RAIELRRAIVRSTNSGSSGFVNLSGDYVEPLLGPRLSGQEVEEVQVWDVPVNWVDPTF
                     YVRFGNLWILILAVGVAGWTTVRVRSRK"
     gene            2910..3482
                     /gene="pyrE"
                     /locus_tag="PKK76_14365"
     CDS             2910..3482
                     /gene="pyrE"
                     /locus_tag="PKK76_14365"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNL69714.1"
                     /translation="MGPLAKELFEATRNHIYQFREEPFTLASGRKSRHYFNCKKITLV
                     PGRLHMLARVLRDEVIPAAGLSPEAVGGLTLGADPIGCALSLAYHEAGKQVVPLIVRK
                     EAKSHGTGQKIEGEWKGVHEVLVLDDVITTAGSTLQAVSAFREAGLVVRHAVCIVNRE
                     EGGEQALREQGITLFQVFKKSDFSLVKDPD"
     gene            complement(3491..>3694)
                     /locus_tag="PKK76_14370"
     CDS             complement(3491..>3694)
                     /locus_tag="PKK76_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69715.1"
                     /translation="EFGAFNEGDKQSAKKLVDDLEGRIIQIEQQYNQFAVKYLQPKQP
                     DGEKPDSILSALQGVAKAIFEKR"
BASE COUNT          885 a          952 c          956 g          901 t
ORIGIN      
        1 accatgacga attcaagatg gttccactgc cagaacgtgc gccgccggac cgtaccactg
       61 aaagcttgca ccggtccccg cgccgatttc cagtatgttc cctttcagat cgcgcagaag
      121 tctgcgtcgc cgccgcgcca attcgacttt ttcgggcttc tccatgaaag tatcatagaa
      181 ccgcgcaaaa attcgatcgt agacgttatt cgacatagac cctagaactc cggggcgaat
      241 tttttcagga agtcatccat ccacgctgca tagagagttg gatttgtttt gatgagaccg
      301 caatgaccgc ccttgtccac aagatgaagc gttttgcgat tgtgcccgat ggcggcgaaa
      361 acttcttctg aatgggtgaa aggaattgca tcgtcgtctt ttacgtgtgt catgaaaacc
      421 gggatttcaa tgtggggcgc cgcagaaagg gggcttgcgt cctctgtgcg aaaattgccg
      481 cgaagccctg cgatccagga tgctgcggga tagagtgcca gaatgggagc ctttccatac
      541 agagtacttc ctctgtattt caaaatagag tccaggtcgc ggaaaggaga atccgctgct
      601 acaaatgcga aatcaggtgt gagtggtgcg gcctgcagta cgatcgccgc tcccatggaa
      661 gcaccaaaga ggccaatctt ctcgtgcgga agtccagtac gctttgcaaa ccagtctgcc
      721 acaatcacaa gatcatcgcg ttcgcgtact ccccaggtgg caaatgttcc ggcactctgg
      781 ccatggtggc gcgcatcgaa cataagcaaa tggcagccgc gtggccagaa tagcttcgca
      841 tacttgagca tgccgttgcg attggcggca tgaccgtggt ggaggatgac gccgcatctg
      901 cgattcttac ggttcacaaa caaccatccg ttcagctcaa cctgaccgcc tgttacggat
      961 acgtcctctg gtctgggcag gtccacgtct gcgtaaccgc gaagattgtc ctctgcgaga
     1021 acagtttcct ttgattttac tttgaaagtt gtgatctctc ctgaaaaata gaacgcagct
     1081 gtggcgtacg caacaaacaa caccatagca atggtcagcg tttttttcat tttcttgatc
     1141 taacccgaac ggttgtccag cctgcaacac ccacggcaag aatcagaatc cagagattcc
     1201 caaatcttac gtaaaacgta ggatctaccc agttcaccgg aacgtcccat acctggactt
     1261 cttctacttc ctggccggag agtctgggtc cgagtaatgg ttctacatag tctccggaca
     1321 gatttacgaa tcccgagctg ccgctgttgg tagatcttac gatcgcacgt ctcagttcaa
     1381 tcgctcgaat gcgtcccagt ccaaaatgct ggtaggtttc cacagttttg ccataccaac
     1441 cgtcttgcgt tatgttcaca atgaatccag gatttgaatc atcaaagtag cttcttacgt
     1501 attccggaat caaaacttcg tagcagataa gaggaaggaa cgtccctgcg ggttgcgcgc
     1561 cttctttttt tacgtagtac gtaagattgt gtgcgctttc acctctttca aaacggctgt
     1621 atcgcactgc ttcgggcaca agtgcgatga gaccggaact atccaggaat tctgcgcccg
     1681 ggatgtattc gccaaaagca agtagagtcc ttttgtggta gtactctcgt cttcctccgt
     1741 caggcgaata aacgatagaa gaattgaatt caatagggcg aggtctgcct gtcacacgat
     1801 ccagtttgac gtctgctgcc acttcgtttg catacacagt tgctttgtat ttcgtcgcca
     1861 gtttttgcat catggacgcg aactccgggc tgtaaatgcc ctcaatgcgc gtgaccatgt
     1921 tgtcctgggt cgtcaaattg ttgggaacag ctgactcagg cagcacaatc atgtctagag
     1981 gtccggccgc ggccgctttt cccacgagcg acgggattct attttcaagg atgtcgcgca
     2041 cttcatcgcg cggaatcaga ccttctccgg cgcgcggaag tcgtgtgttt ggttgtatca
     2101 tggccacgcg aacatgagga gattgttttt cgacgttcgc catctggtag agacgcacat
     2161 aaccccacac caacacaatg gcgaataacg tgggaacggc ccactttccg cggttgtcgc
     2221 tccaggcgcc gctcttcagt gcactcatca ctgcttccga tgctttctct tggccctggg
     2281 cgattgaatg caggatcgtc ttgaacagtg catagagagc gtagctgcct gcgaaatgca
     2341 gaaaagatat cccgtatact cctgcgaatt ccgctacctg ggacaaaggt ctgttcttac
     2401 cggccagatt tccccagtac caggggaata cctgaggtgc caggtaatcc gtgagcacgc
     2461 agagagtggc cgcgaccagc cacatgggag gtagctttgt ttcgaatctc ttggcgaaac
     2521 caaatgctac cagaaacatt ggaatcttca aattgagaag gatcgtgtat gggatgaata
     2581 tcaagagtcc tagagcaatg ttgagtccgc cgtacgtgct aaagaggtgg agcatccagt
     2641 aaaaggaaaa caggcaaaga aacatcgcgc cgagtgctcc aattcccaga agcttccacg
     2701 catgattctg atatcgatcg gcgaaatgaa acagcggcca tgaacaaatc caaacaatgc
     2761 ctggaataag gaatggatcg agcgaaagcg tacagaggcc tccgaagaca atgcagcctg
     2821 cgatgacctc gatccatgga tttgcaaaaa agcctggcaa aggcaggcct tcgtttttct
     2881 tcgtattacc ggccgatcga ggagatgcca tgggacccct tgctaaagaa ttgtttgaag
     2941 cgacacgcaa tcatatttat caatttagag aggagccgtt cactctggca tcaggccgga
     3001 agtcgaggca ttacttcaac tgcaaaaaaa tcactcttgt cccgggtcgc ctccacatgc
     3061 ttgctcgagt tttacgagac gaagtcattc ctgcggcagg cctgtctccg gaagcagtgg
     3121 gtggtctaac actgggcgcg gacccaatcg gttgcgctct ctctctagcg tatcatgaag
     3181 ctgggaagca ggtggttcct ttgatcgttc gcaaagaggc caagtcgcac ggcacaggtc
     3241 agaagatcga aggtgaatgg aagggcgtac acgaagtgct ggtgctggac gatgtcatca
     3301 ctacagctgg ctccactctt caggcggtga gcgcgtttcg cgaagcgggc ctggtggtgc
     3361 gtcatgcggt gtgcattgta aatcgcgagg aaggcggaga acaagcactg cgcgaacagg
     3421 gcatcactct ttttcaggtc ttcaaaaaga gtgatttctc cctggtaaag gatccggatt
     3481 aaagtctcgc tcagcgcttc tcgaagatcg cttttgcaac tccctgcagt gcgcttagga
     3541 tggagtctgg cttttcacca tccggctgct ttggctgcag gtacttcacc gcaaactgat
     3601 tgtactgttg ctctatctgg atgatgcgac cttcgagatc gtccacgagt ttttttgcgg
     3661 attgtttgtc cccttcattg aaggcaccga attc
//
LOCUS       DAORFF010000401         3693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15463_length_3693_cov_3.498351, whole genome shotgun sequence.
ACCESSION   DAORFF010000401 DAORFF010000000
VERSION     DAORFF010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15463_length_3693_cov_3.498351"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PKK76_14375"
     CDS             <1..677
                     /locus_tag="PKK76_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69716.1"
                     /translation="GRTNPATAPVALGTVPGDTVFAAAPIRFQTPVSQISAGFSRDLT
                     VQVRGNASATYYPVAAETLTFAGAKGTTARPTWLSARYTAQKIGSSFLEATYGTLNLS
                     VPINVYPGVLSRIEVLYNDAVLSDPFNVNAGTVKQLDAAGYDSFDNFMFVQPVFSVAG
                     GIGSFGGPANYGQFTAAPLPADTTGTITATLGGGFSVTYNVLVHGSGASCQFDVGHFD
                     TSCVFN"
     gene            732..1292
                     /locus_tag="PKK76_14380"
     CDS             732..1292
                     /locus_tag="PKK76_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNL69717.1"
                     /translation="MKTTNKIMEYIKSGFFKGVGLGVGLLTTSIFAAAAAINVFSPGQ
                     VISAAQINQNFAIAAPEGAVMAFYLTSCPDGWAPADGTNGTPDLRGQFIRGRDDVGTG
                     PAGMDPDGSRVIGALQADAFQGHVHQSRVWLGNPNNFLPAGGLQELSKDGPTGGPMSD
                     GVNGTPRTANETRPKNVALTYCMRKN"
     gene            complement(1382..1762)
                     /locus_tag="PKK76_14385"
     CDS             complement(1382..1762)
                     /locus_tag="PKK76_14385"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNL69718.1"
                     /translation="MLRVRTSVRPSQIAGLGLFAAEDISKDQIIYEWTAKFEQTFTAE
                     EYEKLGPLQKEFLRHYSFKRSGIRVVSLDDDRFANHSDTPNTYEKGADTHALRDIAAG
                     EEITCNYYDFDESADEKLAGIQRS"
     gene            1989..2795
                     /locus_tag="PKK76_14390"
     CDS             1989..2795
                     /locus_tag="PKK76_14390"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNL69719.1"
                     /translation="MRQALLVFLSFLCCNAPRSAPLNPPAAGLSTVDVGGVPCDVFVP
                     DAPVGTVLVLPGWNFDRGSWRKNSSIEEQARKHHLILILPDMLKTLYETSYYPETTLR
                     WNKTPGSQFIHDTLIPEMQKRYSLLMPGQRNFVLGLSTGGRGVAMTILENPKLFAAGA
                     AFSGDYAQERMPGEAINAAIWGPYALFPDRWKGRDNPARRASEWQTPIYLAHGLDDRV
                     VPPDQTQMFGDLLSARDKTLVEVHLKAGYGHDYRFWDSQLAPAFEFFSRH"
     gene            2802..>3693
                     /locus_tag="PKK76_14395"
     CDS             2802..>3693
                     /locus_tag="PKK76_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNL69720.1"
                     /translation="MSVRFEVSGDSLRITFDGPLTFETAGSMFPEIMHKLKTGVRGIS
                     VDAHRVDAMDTAGAALLIHLEKHQSTLDLPFEIVGLAESYRKLYHLFDRAILEQTWED
                     EEIRWHFDAIGRRTVSLWEKVKEFTAFCGELFVALMKSISNPSRIRWKDVFITCESAG
                     VGALPLIGMIGFLIGLILAFQSAIPMRRFGAEIFVANLIGLSMIRELGPLMTSVILAG
                     RSGSAFAAEIGTMRINEEIDAIVTMGFDPMKFLVVPRIIASLIVAPLLTLFFQLAGLA
                     GGAVVMLSLGFPFVTYVQQVQ"
BASE COUNT          832 a          924 c          971 g          966 t
ORIGIN      
        1 caggccgcac gaatccggcg accgctcctg tggctctcgg gactgtgcca ggcgatactg
       61 tgtttgccgc agctcccatt cgttttcaga ctcccgtttc gcagatcagc gcgggtttct
      121 cgagagatct gacggtgcag gtgagaggca atgcttccgc aacctactat ccagtagcag
      181 cagagacatt gacattcgcc ggtgccaaag gcacgactgc tcgccccacg tggctcagtg
      241 ccagatacac tgctcagaag attggatcat cgtttctaga agcgacgtat ggaactctca
      301 atctctcagt gccgattaac gtctatccag gagtactcag tcgcattgaa gttctctaca
      361 acgatgcagt gctttctgat ccgttcaacg taaatgcggg tacagtcaag cagctggatg
      421 cagccggata tgattcgttc gacaacttca tgttcgtgca gcctgtcttc agcgttgccg
      481 gcggtattgg ttcgttcggt ggtcctgcca actacggaca gttcactgca gcacctctgc
      541 ctgcggacac taccggcaca atcacagcga ccctcggagg cggtttctcc gtgacttaca
      601 acgtgctcgt acacggaagc ggagccagct gtcagtttga cgtggggcat tttgatacgt
      661 cttgcgtgtt caactaacgt gagaaacgat ttgcaattta gtatgaaaat ttaaagaatg
      721 ggaaaagaaa aatgaaaaca actaacaaga tcatggaata cattaagagt ggatttttca
      781 aaggcgttgg ccttggagtt ggtctattga ccacaagtat ctttgccgcg gcagccgcca
      841 tcaacgtatt ttcgcctggt caggtgatca gtgcagcgca gatcaatcag aactttgcaa
      901 ttgccgctcc agaaggcgcc gtgatggctt tctacctgac cagttgcccg gatggttggg
      961 cgccagcaga cggtacaaat ggaactccag atctgcgcgg tcagttcatt cgcggtcgcg
     1021 atgatgtggg aaccggtccg gccggcatgg atccagatgg aagtcgcgtc attggggcgc
     1081 tgcaggccga cgcattccag ggtcacgtgc atcaatccag agtctggcta ggaaatccca
     1141 acaacttctt gccagcggga gggcttcagg agctttccaa ggatggacct acaggtggcc
     1201 caatgtcaga cggagtgaat ggcacacctc gcactgcaaa tgaaacacgt cccaagaacg
     1261 tagcgctcac ctactgcatg cgtaagaact gagtttatga atgaagtttg caaggcgagg
     1321 tgactcgctt tgctaaatca aaatcaagac tcttgcgcgg atgtgcgaga gtcttcttgt
     1381 tttacgaacg ctgaattcca gccagtttct catccgcgct ttcgtcaaaa tcgtagtagt
     1441 tgcatgtgat ttcttcaccg gctgcaatat cgcgcagcgc atgagtgtct gcacctttct
     1501 catatgtgtt gggagtgtca ctgtgattgg caaatcgatc atcatcgagg gacacaacac
     1561 ggattcccga tcgtttgaaa gaatagtggc gaagaaattc tttctggagc gggccaagtt
     1621 tctcgtactc ttctgcagtg aaggtttgtt caaacttcgc agtccattcg tagatgatct
     1681 gatctttgga aatgtcttcc gccgcaaaca gccccaggcc ggctatctga ctggggcgga
     1741 cgctggttcg aactcgtagc acgcttcttc tcgtcagccc cctggttgtg cgcaatcata
     1801 aaactacgat ttcgctattg tgtccctggc acattttctg tttttgtttg cggccgcgcc
     1861 aaccagggaa agaatctcag aacccggtat gagacataat gcaagttatc ggaaccggag
     1921 tgttttgaac aagctggatg catgcaaatt cttgatgcct tgcaggcatg cccctggact
     1981 gtcgtggcat gcgacaggca ctccttgttt tcttaagctt cctttgttgc aacgctccca
     2041 ggagcgcacc tttgaatcct cctgctgccg gtctttccac cgtagatgtg ggcggagtgc
     2101 cttgcgatgt ttttgttcca gacgcacccg tgggaacggt tctggttttg ccaggctgga
     2161 attttgacag aggatcgtgg agaaaaaact caagcattga ggaacaggca cgcaagcatc
     2221 atctcattct gattttgccg gatatgctca aaactctcta cgagacatct tactaccctg
     2281 aaacaactct gcgatggaac aagaccccgg gctctcagtt cattcatgat actctcattc
     2341 cggagatgca gaaacgttat tcgctcttga tgcccgggca aaggaacttc gttctgggtc
     2401 tgtccaccgg aggacgtggt gtggctatga ccatacttga gaatcctaaa ctatttgctg
     2461 cgggtgctgc tttctctgga gactatgccc aggagagaat gccaggagaa gcaatcaatg
     2521 ccgcaatctg gggaccgtat gcactgtttc cggatcgatg gaaaggccgc gataaccctg
     2581 cccgacgcgc ttcagaatgg cagactccta tttatctggc acatggtctg gacgatcgtg
     2641 tggttccacc ggaccaaacc cagatgttcg gagatctttt gtccgcacgc gataagacac
     2701 tcgttgaggt gcatttgaaa gctggctacg gccatgacta tcgcttctgg gattctcaac
     2761 tagctccggc tttcgaattt ttttccaggc attgaactgc gatgtctgtt cgttttgaag
     2821 tttctggaga ttctcttcgc atcacattcg acggccccct caccttcgag actgccggaa
     2881 gcatgtttcc agagattatg cacaaattga agactggtgt gcgcgggatc tctgtggacg
     2941 ctcaccgggt ggacgctatg gacacggcgg gggctgccct cctcattcat ctagagaagc
     3001 atcaatctac cctcgacctg ccttttgaaa tagttggcct ggcagaatcg tataggaaac
     3061 tatatcatct gttcgatcgt gcaatcctgg aacaaacctg ggaagacgaa gagatcaggt
     3121 ggcactttga tgcgattgga aggcgtacgg tcagtctctg ggaaaaagtg aaggaattca
     3181 ctgccttttg tggcgagttg tttgtggctc tgatgaaatc tattagcaat ccttcgcgca
     3241 tccgatggaa agatgtattc atcacctgcg aaagcgctgg agtcggggca cttcctctga
     3301 ttgggatgat tgggtttttg atcgggttga ttcttgcgtt tcaatctgcc attcccatgc
     3361 gtcgatttgg cgcagagata ttcgtcgcaa atctgattgg tctttctatg attcgcgaac
     3421 taggaccgct catgacttcc gtgatccttg ccgggcgatc tggttctgcg tttgccgcgg
     3481 aaatcggaac gatgcgaatc aatgaggaga tcgatgcgat tgtaacgatg ggattcgatc
     3541 ccatgaaatt cctggtggtt ccgcgcatca tcgcatcgtt gatcgtcgca ccactcctta
     3601 ctttattctt tcaactcgcg ggactcgcgg gtggtgcggt tgtgatgcta tcgcttggct
     3661 ttccttttgt aacctacgtg cagcaggtac aat
//
LOCUS       DAORFF010000402         3690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15495_length_3690_cov_3.268501, whole genome shotgun sequence.
ACCESSION   DAORFF010000402 DAORFF010000000
VERSION     DAORFF010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15495_length_3690_cov_3.268501"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..582
                     /locus_tag="PKK76_14400"
     CDS             148..582
                     /locus_tag="PKK76_14400"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNL69721.1"
                     /translation="MHWLVWIVGGLVVAFAELAIPGLIALFLGGGAVVTGVLVYFGWL
                     DSISSQLSAWICISLALFLLFRDQVMKRFPSLEKRDAITEDQVMIGKIVPVIEQITPE
                     KPGRVSVHQSSWKAVSSSTIEKGENARIIARDQLVLTVEKAN"
     gene            635..1579
                     /locus_tag="PKK76_14405"
     CDS             635..1579
                     /locus_tag="PKK76_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNL69722.1"
                     /translation="MMFLAAVGLILFLLFLKLTLVIVPHQHAYIKERLGSFAGVLNSG
                     WHILIPIADRIAYKHLLMEEVIDVPPQICITKDNVQVEVDGILYIRVLDPKLASYGIQ
                     DYKFASIQLAQTNMRSEVGKLDLDRTFVEREKINDAIIRSLDHASEPWGVKVVRYEIK
                     NIIPPKTILVTMEKQMTAERDKRADIAHSEGERAARINHSEGEKLEAINLSEGEKQKR
                     INEAAGNAAQIELVASATADGLREVAGAMQHPSGARAVQMRIVEDYIKQFGAVISQAQ
                     TSVVPVGLANIQGIMAGMSELLSKGQVVTPPPAPSKDA"
     gene            1590..2510
                     /locus_tag="PKK76_14410"
     CDS             1590..2510
                     /locus_tag="PKK76_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HNL69723.1"
                     /translation="MDAVGQLVFTAFAIYVLIKFALSIRIVPAQQVFLVERLGKFHKQ
                     LGAGFHSMIPFLDRVTYKLNLKEEAIDVPAQTCFTGDNVQIQVDGVIYMKVEDPYRAA
                     YNVTNYHYALIQLAQTTMRAALGHLELDKTFEERESLNAQIVKVVDEAAEPWGINIMR
                     YEIQNITPPKSILQSMEKQMTAEREKRATIARSEGEKTARINTSEGLKQELINRSEGE
                     KQRTINQAEGQAAEIRAIALATAAGLKKVADAVAAPGGAEAMRLSLVQEYLRMMEGLA
                     KSDTKVVLPVNLADLKETLGGLGLKSKAQE"
     gene            2507..3280
                     /gene="cmoB"
                     /locus_tag="PKK76_14415"
     CDS             2507..3280
                     /gene="cmoB"
                     /locus_tag="PKK76_14415"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011650.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HNL69724.1"
                     /translation="MNYTVEAGVVTLGPPESELDSLRQRALSLGPWRKGPFRVGGLLI
                     EGHWKSDLKWNRAIRAAGDIRGKTVIDVGCNNGYYLFRMREAGASHAIGFDPVAQFEN
                     QFLFLNEFARDPSIDFIRSGYQEMGAATADIVFCMGVIYHQKNPMDVLEKLLGALRPG
                     GRIILETMGLDIGENCLVPSGRYAGSSGIWFVPGPGAVANWCKRTGLKCTLADVWDAR
                     DEQQKTQFAELPLTTDFYHGEKTSEGYPLPLRYLFLVER"
     gene            3355..>3690
                     /locus_tag="PKK76_14420"
     CDS             3355..>3690
                     /locus_tag="PKK76_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69725.1"
                     /translation="MKQLSIAVTIFLFAFSIHAEEESEARVIAPIKKLIGSVRYSEKA
                     STSAEKKTLEDRALEYIGSAQMADFLLGDFSAKATPEQKEKFASQIREYTRYKAFPLV
                     NKYFKDIDLT"
BASE COUNT          995 a          867 c          973 g          855 t
ORIGIN      
        1 cgctccgctt aagaaagcag tgccagatga gcatctggct gaagttcagg ctgaaccaga
       61 agcagaagag attccgtttt ctatcggtgg aaaaatcgac ttgaacaaca tgagcgtgcc
      121 cgcatatctt cgtaagcaac agagataatg cactggcttg tttggattgt tggcggactg
      181 gtagtagcgt tcgcggaact cgcgattccc ggattgatcg ccctgttcct gggcggtggt
      241 gctgtggtca cgggagtgct cgtctacttt ggatggctgg atagcatttc ttctcaactt
      301 tctgcgtgga tttgcatttc tctggcgctc ttcttactct ttcgcgatca ggtgatgaag
      361 cgttttccgt ctctggagaa gagagacgcc atcacagaag atcaggtgat gatcgggaaa
      421 atcgttcctg tgatagagca aatcacgccg gagaagcccg ggcgtgtttc cgttcatcag
      481 agttcctgga aagctgtttc ttcttctaca attgagaagg gtgagaacgc tcgcatcatc
      541 gcaagagatc aacttgttct gacggtcgaa aaagcaaatt aaccaaaagc aattgacgat
      601 tcatcgggcg tccacagaac acgcttcgga gaatatgatg tttcttgctg ccgttggact
      661 gattctattt ctattgttct taaagctcac actggtgatt gtgccgcacc agcacgcgta
      721 catcaaagag agactcggca gttttgccgg cgtgctcaat tctggttggc acattctaat
      781 tccaatcgca gatcgcatcg cgtacaaaca tcttctcatg gaagaagtga tcgatgttcc
      841 accccagatt tgtatcacca aagacaacgt gcaggtagaa gtggatggaa ttctctacat
      901 tcgagttctt gatccaaagc ttgcaagcta cggaattcag gattacaagt ttgcgtccat
      961 tcaactggct cagaccaaca tgcgttccga agtcgggaaa ctggatctgg atcgcacctt
     1021 cgtggaacgc gaaaaaatca acgatgcaat cattcgcagt ctcgaccacg caagcgaacc
     1081 atggggtgtg aaagtcgttc gatacgaaat caaaaacatc atcccgccca aaacaatcct
     1141 cgtaaccatg gaaaagcaga tgactgcaga gcgcgacaag cgcgcggaca tcgctcattc
     1201 agaaggagag cgggcggctc gcatcaatca ctctgaaggt gaaaaactcg aagcgatcaa
     1261 cctgtcagaa ggagagaagc aaaaaagaat caacgaagcc gcggggaatg ccgctcagat
     1321 cgaactggtc gcgtctgcga ctgcagatgg ccttcgcgaa gtagcgggag cgatgcaaca
     1381 tccttccgga gctcgcgccg tacaaatgcg cattgtggaa gactacatca agcaattcgg
     1441 cgctgtcata tcccaggcgc agacatctgt tgtgcctgtt ggtctggcaa acattcaggg
     1501 tatcatggcc ggaatgtcgg agctactgag caaagggcag gtggtcactc cacctccggc
     1561 accttctaag gacgcataag gagaaaatca tggatgctgt tggacaatta gtatttaccg
     1621 cttttgcaat ttacgtcctg atcaaatttg ctctgtcgat tcggatcgtt ccggcccagc
     1681 aggtgtttct tgtagaaagg ctgggcaaat ttcacaaaca actgggcgcc ggctttcatt
     1741 ctatgattcc ttttttggat cgagtgactt acaaactcaa tctgaaagag gaagccatcg
     1801 acgttcccgc acaaacctgt ttcacgggag ataacgtgca gatccaggtg gatggagtca
     1861 tctacatgaa agtagaagat ccgtatcgtg ctgcctacaa cgtaaccaat tatcactatg
     1921 cattgattca gctggcacag accacaatga gagcggcgct cggccatctc gaactggaca
     1981 agacatttga agaacgcgaa tcattgaatg cacaaattgt aaaggttgtg gatgaagcgg
     2041 cggaaccatg gggcatcaat atcatgcgct atgaaatcca gaacatcact cctcctaaat
     2101 ccattctgca gagcatggaa aaacaaatga ctgcggaaag agaaaagcgc gcgacgattg
     2161 caagatcgga aggcgagaaa acggcgcgca tcaatacatc tgaaggtctc aaacaggaat
     2221 tgatcaatcg ttctgaaggt gaaaagcaga gaacgatcaa tcaggcggaa ggtcaggcgg
     2281 ctgaaattcg tgcgatcgct ctagccactg cggccggatt aaagaaagtg gcggatgctg
     2341 tggcggctcc tggtggcgcg gaggcaatgc gtttgagtct cgtccaggaa tacctgagga
     2401 tgatggaagg tctggcaaag agcgatacaa aagtagttct gccagtgaat cttgccgact
     2461 tgaaagaaac gttaggcggc cttggtctga agagcaaagc tcaggaatga attacacggt
     2521 ggaggccggc gtagtaacgc ttggcccgcc cgagtcagag cttgattctc tccggcaaag
     2581 agccctctct ctgggaccct ggagaaaggg tccgtttcgc gtgggtggtc ttctgataga
     2641 aggccactgg aagtctgatt taaaatggaa tcgcgccatt cgtgccgccg gagatattcg
     2701 cggcaagacc gtcatcgacg ttggctgcaa taacggatac tacctgtttc gcatgcgaga
     2761 ggcgggagcg tctcacgcga taggctttga tccggtcgcg caatttgaaa atcaattcct
     2821 gtttctaaac gagtttgcca gggacccttc gatcgacttc atccgatccg gctatcagga
     2881 gatgggcgct gcaacggcag acattgtgtt ctgcatgggt gtaatctatc accagaaaaa
     2941 cccaatggat gttctggaga aattactcgg agcgctgcgt cctggtgggc gaatcatcct
     3001 ggaaaccatg ggacttgaca tcggtgaaaa ctgtctggtt ccatccgggc gttatgcggg
     3061 gagttctggc atctggtttg ttccgggccc gggagcggtt gcaaactggt gcaagcgaac
     3121 tggcctgaaa tgcactctgg cagacgtctg ggatgcaaga gatgaacagc agaagacaca
     3181 attcgctgaa cttccgctca ctactgattt ttatcatggc gaaaaaacga gcgaaggcta
     3241 tccattgccg ctgcgctatc ttttcctcgt cgaaaggtag aggaaattgg ttgtcaaagg
     3301 tactttggca ctaaaataag ggagccaaaa atccagtctg tccgtccaaa cactatgaaa
     3361 caactctcaa ttgcagttac aattttcctc ttcgcatttt ccattcatgc ggaagaagag
     3421 agcgaggcac gcgtcattgc acctatcaag aagttgatcg gcagtgtgcg atacagcgaa
     3481 aaggcaagca cgagtgcgga aaagaaaact ctagaagatc gcgctctgga atacattggg
     3541 tccgcgcaga tggcagattt tctgctcggg gatttttctg caaaggccac cccggagcaa
     3601 aaagaaaagt tcgcttctca aatccgcgaa tacactcgtt acaaagcatt tccactcgtg
     3661 aacaagtatt tcaaagacat tgatctcacc
//
LOCUS       DAORFF010000403         3677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15585_length_3677_cov_3.304252, whole genome shotgun sequence.
ACCESSION   DAORFF010000403 DAORFF010000000
VERSION     DAORFF010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15585_length_3677_cov_3.304252"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="PKK76_14425"
     CDS             complement(<1..1150)
                     /locus_tag="PKK76_14425"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNL69726.1"
                     /translation="MSVVRIAGIDFPVSERETEWLPSANLVLTPTTIRNLQKILHPLR
                     AGIPLLLVGDAGVGKNALIYYINALRKHPTIRYSFNEDTLPEDLVGAYRIDPGHSFVW
                     SDGPLTHAIRTGSVFVADEMNLAPPEVLKRFLSVFSEGSIQILEGDSSTLRPAPGFHF
                     IATQNPAEGFEGRKNLPREIQKYFATVYVDAYPDSEVIEILCGTHPTVRADLVEAIVR
                     INGKVETALVKREIGARDLERYHFNLRNMNRLCYRIAGSDGSVAAVQREIYDVYVLPF
                     RNPEDQKIVKEIIHSVIPDRAEESSVRISVGGDAISIGHAILKPAVIDRARIEDSFNH
                     FPPVESRLPLLASIARSVEMHENVLLESDADVEPEEYAEFFARMMGAEL"
     gene            1222..2316
                     /locus_tag="PKK76_14430"
     CDS             1222..2316
                     /locus_tag="PKK76_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNL69727.1"
                     /translation="MLKLSERMSRIDTSKIRRAFDLAAKLENPINLSIGQPHYATPEP
                     ITEAIAAALKDGKTAYTPTQGILPLRERIARKFLEQNKFAASPEHILISSGISSLIQL
                     LFLATIDPGTRVLITDPCFLIYRSLLRYFNANVTTVPEEFTQEDLDRLPPGDFSLIIF
                     ATPSNPTGYVMKKSQIEALAKFADARNALLVSDEIYELFDYEGQFTSAASVHKNTITL
                     SGFSKTYSMTGLRLAAAHGPEAIIKAMTTLQQYTVVCAPAPVQWAGIRALDLDMSSYV
                     KAYKKNRDACVSALSGRVKFPEPGGAFYIFPDVMEDEDSFVERAINEKQLLVVPGSIF
                     CAERTRIRISYAASPETLERGLAAFVSLLK"
     gene            2313..3122
                     /locus_tag="PKK76_14435"
     CDS             2313..3122
                     /locus_tag="PKK76_14435"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNL69728.1"
                     /translation="MSEELLLPFAYVFAGLLGAILGSFYYATATRVSYFFYSAARKSY
                     PRKRDRFRALIFKPSFCMECGAPISRVYLIPVFGYAFSRARCAECGTRISPKHLVAEI
                     YLAILLPTLLYFGRSWPEALLMTLFAGQLYVSIVTDTRHMILDHENTAFLFLWAAILT
                     AIADGTTARLIGGGGTLVIFGALYWISRLTGRTMMGFGDVPLSAAVGLFFGFPTLLIV
                     FVAAACGSIFYSLVIQGSRNAPAPFGASLGFATLTAIPLQIVAQWAGFLQF"
     gene            complement(3127..>3677)
                     /locus_tag="PKK76_14440"
     CDS             complement(3127..>3677)
                     /locus_tag="PKK76_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNL69729.1"
                     /translation="YFGEASLILERPRSADVIAETDVIALTIEKSRFLNFIKGSDLLD
                     KFAQLNEIRKTGTWEVLSQSRLLGGITSAQKTQLELIMDRKSFRAGSYLIEQGRNFYE
                     AFIIKHGTVEVLSNGRVVEKLGRGDFCGEIFCLQKESPSTLTFRAVSDIDVYAIDRPR
                     LVHYIADNPGVYMRLNYVYGAS"
BASE COUNT          894 a          971 c          893 g          919 t
ORIGIN      
        1 tgagctccgc gcccatcatc ctcgcgaaga attcagcata ttcctctggc tccacatccg
       61 cgtcgctttc gagaagcacg ttttcatgca tttccacaga acgcgcgatg gaagcaagaa
      121 gcggcaatcg actctctacc ggtggaaaat gattgaaaga atcttcaatc cgagcgcgat
      181 cgatcactgc aggcttgagg atggcatgtc caatggagat tgcatcccca cccacggaga
      241 ttcgcacgga tgattcctct gcacgatctg gaattacaga atggatgatt tcttttacta
      301 ttttttgatc ttcaggattg cggaaaggga gaacatacac atcataaatt tcgcgctgca
      361 cggcggccac ggatccgtct gaacctgcaa tgcgatagca caggcgattc atattgcgca
      421 ggttaaagtg atatctctca agatctcgcg caccaatctc gcgctttaca agtgccgttt
      481 ctactttgcc attgatgcgc acaattgctt ctaccagatc cgcgcgaaca gttggatgag
      541 ttccgcagag gatttcgatt acttccgaat ccggatacgc atccacatag acagtggcga
      601 aatacttctg gatttcgcgc ggtaggtttt ttctgccctc gaatccttcc gcgggattct
      661 gcgttgcgat gaaatgaaaa ccaggagcag gcctgagagt agacgaatct ccttcaagaa
      721 tctggatgct gccttctgaa aaaacggaca ggaatcgctt caacacttct ggcggcgcca
      781 gattcatttc atcagcgaca aagacagaac cagtgcgaat cgcatgagtc agcggaccat
      841 cggaccagac aaaagaatgg ccggggtcaa tgcgatacgc acccacgagg tcttctggca
      901 gagtgtcttc attgaaactg taacgaatgg ttgggtgctt tcgcagagcg ttgatataat
      961 agatgagagc gttcttgcct acacccgcgt cccctacgag caagagagga atgccagcgc
     1021 gaagaggatg caggatcttc tgcaaattgc gaatggtggt gggtgtaagg acaaggttag
     1081 ccgaaggaag ccactcggtt tcccgctcgc tcactggaaa gtcaattccg gctatgcgca
     1141 caacgctcat actctaaagc ggacctgaaa ccatcccgcg cccaggctgg ttcttggaaa
     1201 caatattttt cggaggatgc gatgctgaaa ctctcagaaa ggatgagtag aatcgacacg
     1261 tcgaaaatca ggcgcgcgtt tgacctggcc gcgaagctcg aaaatccaat caatctgtcc
     1321 attggccagc cgcactatgc aactcctgaa ccaatcacag aggcaatcgc ggccgcgctg
     1381 aaagacggaa aaacggccta tacgcccact cagggaattc ttccgttgcg agaacgaatc
     1441 gcgcgaaaat ttttagagca aaacaagttc gctgcatctc cagagcacat cctgattagc
     1501 tccggcatct ccagtctaat ccagctcttg tttctggcaa cgatcgatcc aggcactcgc
     1561 gtactgatca cggatccctg ctttcttatt tatagaagct tgcttcgcta tttcaatgcg
     1621 aacgtgacca ctgtcccgga ggaatttaca caggaagatc tggatcgtct gcctcccggc
     1681 gacttctctt tgatcatctt tgccacacct tctaatccaa ctggttatgt gatgaagaag
     1741 agccagattg aggctctcgc aaagtttgca gatgcgcgaa acgcacttct tgtatcagac
     1801 gaaatctatg aactgtttga ttacgaaggc cagttcacga gtgctgcctc cgtccacaag
     1861 aacacaatca ctctaagcgg cttttcaaaa acatacagca tgacgggtct tcgcctggcc
     1921 gcggcgcacg gaccagaagc gatcataaag gcaatgacta cactgcaaca gtatactgta
     1981 gtttgcgcac cggccccggt ccaatgggca ggcattcgcg ctctggacct ggatatgagt
     2041 tcgtacgtga aggcttacaa aaagaatcgg gatgcatgtg tgagtgctct ctccgggcgt
     2101 gtgaagtttc ccgaaccagg tggcgcgttc tatatctttc ctgacgtgat ggaagatgaa
     2161 gattcattcg ttgagagggc tatcaatgaa aagcaacttc tggtagtgcc cggcagcata
     2221 ttctgcgctg agcgcacacg aattcgcatt tcgtacgcgg catcccctga gactttagag
     2281 agaggcctcg cagctttcgt gagccttttg aaatgagtga ggagctcctc ctccctttcg
     2341 cttacgtctt tgcagggttg ctcggtgcca ttctgggcag cttctactac gcaaccgcaa
     2401 cgcgcgtgtc gtatttcttc tattccgcag cgcgaaaatc ctatccgcgc aagagagatc
     2461 ggtttcgcgc gctcattttc aaaccgtctt tttgcatgga atgcggagca cccatctctc
     2521 gtgtatattt gatccctgtt tttggatatg cattctcacg cgcacgatgc gcagagtgcg
     2581 gcaccagaat ctcacccaaa catttagttg cagaaattta tcttgccatt ctgctgccca
     2641 ctctcctcta ttttggcagg tcctggccag aagctctcct catgactctg tttgccggcc
     2701 aactttatgt gagcatcgtc acagatactc gacacatgat ccttgatcat gaaaacacag
     2761 cctttctttt cttgtgggca gccattctca cagcaatcgc agatggaacg accgctcgcc
     2821 tgatcggagg tggtggaact ctagtcatct tcggagcgct gtactggatt tcacgtctga
     2881 ccggtagaac catgatggga ttcggtgatg ttcctctctc tgccgcggtc ggtctgtttt
     2941 tcggattccc aactctactg attgtatttg ttgccgccgc atgcggtagc atcttctaca
     3001 gtctggtcat ccagggatct agaaatgcgc ccgccccgtt tggcgcaagc ctcggctttg
     3061 ctactctgac tgcgattccg ctacaaatcg tggcgcagtg ggcaggcttt ctgcaattct
     3121 agtcgatcag gaagcgccgt agacatagtt caagcgcata tacacacccg gattgtctgc
     3181 gatgtagtgt acaagccgtg gcctgtcaat ggcgtataca tctatgtccg agacggcgcg
     3241 aaacgtcaaa gtagatggcg attccttttg cagacagaag atctctccgc agaaatctcc
     3301 tctgcccagc ttctccacta cgcgtccgtt tgatagaact tccacggtcc cgtgtttgat
     3361 gataaacgct tcgtagaaat tacgtccctg ctcgatcaaa tacgaacctg cacggaagct
     3421 cttacgatcc atgatcaatt caagctgcgt tttctgcgcg gaagtgatcc cgccaagaag
     3481 acgagactga gagagaactt cccaggttcc ggtctttctg atttcattga gctgtgcgaa
     3541 cttatcgagt agatccgaac ccttaataaa atttaggaat cttgactttt caatggtaag
     3601 agcgatcaca tccgtctctg caattacatc cgcagagcga ggacgctcca ggatcagtga
     3661 ggcttccccg aaatact
//
LOCUS       DAORFF010000404         3672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15618_length_3672_cov_3.132154, whole genome shotgun sequence.
ACCESSION   DAORFF010000404 DAORFF010000000
VERSION     DAORFF010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15618_length_3672_cov_3.132154"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..1102
                     /locus_tag="PKK76_14445"
     CDS             251..1102
                     /locus_tag="PKK76_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69730.1"
                     /translation="MESVVGVLVHQGDRMYLSGEDGRRHFEDDPVWPGYAIHWTGRRL
                     KGRLLPQIDYATGKKMVILWPDEPPARPSVELIYNERLTHYWESMMGHIAINVNGEIF
                     NFSNLVNENETMTPEEYYYRPALGEFAPHPVRGKIGTDEEGRPYYDKFGRLFMRSVHI
                     LRIFDIDPQVVRNHLQEVLKEIHAAPRDPKKPENYAQFSVLKRSCATYTRDALRAAGL
                     KKIAGIMPRDLFVNAAYHTTVKRHGSAEIIQKPQLVVPEAPRSAPTALLNPLNRLRNS
                     SISYKPQ"
     gene            complement(1076..1612)
                     /locus_tag="PKK76_14450"
     CDS             complement(1076..1612)
                     /locus_tag="PKK76_14450"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNL69731.1"
                     /translation="MDAESLIEEVRKEYSALDAKIADFVATTGLACPSGCGRCCENPN
                     VTATPLEAILLAASMPPNGTVPDSSICMFYESKGNGRGQCLAYEARPLVCRLFGFASH
                     RKRDGSIGFRPCSVHSHEAPDQTAAAVESAVTAPLYSDWSSRLRSIRPGWMNEQMPIN
                     HAVKEAFEFRLLRFIGNG"
     gene            1754..2425
                     /locus_tag="PKK76_14455"
     CDS             1754..2425
                     /locus_tag="PKK76_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69732.1"
                     /translation="MSCQTQTRAPEALPSILIVGDSIILGKLGEYLETELGRRGHEVH
                     RHAMVSSGLSGLHDYNWPEATYDYIDRYHPDLLIAMFGANDSLAVKNYADDAKFTFFS
                     DSGFRERYARKVRLFLSLNAPRVKTVYWVGQPNLDHHELTPKFPVLNEIYAQEVARVP
                     NGVFIPVWDFTSNAGKYKPVMADATGVVGPLKWDFVHFTPHGGKVLGDHIIEAIYNHR
                     RSHDR"
     gene            2415..>3672
                     /locus_tag="PKK76_14460"
     CDS             2415..>3672
                     /locus_tag="PKK76_14460"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNL69733.1"
                     /translation="MIVEFRKAAFVFIVAGVTTLAALLAERPEVPAEVRSWLPRINDL
                     APDLPGFPWPGAKNSPSDHRTAPGEATSRDLKTGGETKTGVPQKEKVLLENEKALEGV
                     LRTLGRKDRPVRIVHSGDSLMWADNVSSRLRRHLQSRYGDGGRGIVSIVNSPDSVVQD
                     IRIEPTGFSLQKISGAFPQGVDSKLGFFSRSSQSIADPANVQFSFPDGSSPIHKARVV
                     WSGNGTVNLASNGKTLDTATKVVEGACRLTVAETGAEGVHAITMQASGLPVIHAAIFE
                     SSSGVSYSALVTMGIHQAWVRTVSDENFICGWRAAAPDLVIFQFGLNESASIDTYYNG
                     FSQSVYETDLRAYYTRLRKALPDAAVLIVGPLDRVKSADGGLVPVAAQTQVREVQRKI
                     AREFGFAFYDTAVALGPSHVSEFMAKG"
BASE COUNT          845 a          926 c         1017 g          884 t
ORIGIN      
        1 cagtttccgg catggcgcgg acttgttcat aggtgagtgc tccgcctgcg tcttcgagtg
       61 agcctacaaa tgattcgggc ggactttcgt tgatcgccgt tacgtccaga actcgagcgt
      121 tcaggggcaa agaggcggcg atgagtatcc agaccgcaaa cctcatggac ggattgtgag
      181 cgaggcgaaa cgtctgaaca gcaattttcg ttctgtttga cagaggctgc aaccgcacca
      241 gacattgagt atggaatcag tagtcggcgt tctagtccat cagggcgaca ggatgtatct
      301 cagcggagag gatggtcgac gtcattttga agatgatcct gtctggcccg ggtatgcgat
      361 tcactggacc ggcaggcgct tgaagggcag gctccttcca cagatcgact acgctaccgg
      421 caagaaaatg gtcatactgt ggccggatga acctccggcg cgtccgagcg tggagctgat
      481 ttacaatgaa aggctgactc attactggga gtctatgatg ggtcacatcg ccatcaacgt
      541 caatggagag atattcaact tttccaatct tgtgaatgaa aacgaaacca tgacacctga
      601 ggagtattac tatcgtcccg cgctgggtga gtttgctccg catccagtgc gcggaaagat
      661 tggaacggat gaagaaggca ggccctacta tgacaagttt ggcagactgt tcatgcgatc
      721 ggtgcacatc ctgcgcatct ttgatattga tccgcaggtt gttcgcaacc atctacagga
      781 agttttgaaa gaaatacatg cagcacccag ggatccaaag aaaccagaga attatgcgca
      841 gttcagtgtg ctcaaaagaa gctgtgcgac ttacaccaga gatgcccttc gagccgcggg
      901 ccttaagaag atcgccggaa tcatgccgcg cgatctattt gtgaatgctg cctatcatac
      961 caccgttaag cggcatggat ctgcggaaat cattcagaag ccgcaactcg ttgttcctga
     1021 agctccgcga agcgcgccca ccgctttact caatccactc aatcgtttgc gcaattcatc
     1081 catttcctat aaaccgcaat agacgaaatt cgaaagcttc tttcacggcg tggttgattg
     1141 gcatttgttc gttcatccac ccgggtcgaa tggatcggag gcgcgaagac cagtctgagt
     1201 aaagcggagc ggtgactgca ctctctaccg cggcagctgt ctgatccgga gcctcgtgac
     1261 tgtgcacgct gcaaggcctg aaaccaatgc ttccgtctct ctttctgtgc gacgcaaagc
     1321 caaacagccg gcacacgagg ggccttgcct cgtaggcgag acattgtccc cggccatttc
     1381 ctttcgattc atagaacatg catatagaag agtccggtac agtgccgtta ggcggcatag
     1441 aagcggcaag caagatggcc tcgagaggtg ttgccgtgac gttcgggttt tcgcagcatc
     1501 gtccgcatcc ggaggggcag gcgagcccgg tcgtggccac aaaatctgca attttagcgt
     1561 ccagcgccga gtattctttt cgaacctctt cgatcaggct ctctgcgtcc atgccccagg
     1621 ggtggagcgg gtattcaaaa tgtcaaggga accatagcga ttgccgattc ctgctttcgc
     1681 ggatacgtgc ccgtttgaat ttcgtccaat gcgggccttg ccctcaatct tacttgtttt
     1741 ttttggcatt ctggtgtctt gccagacaca gaccagagcc ccggaagcac tcccgtcgat
     1801 tttgattgtg ggggattcca tcattctggg gaagcttggc gaatatctgg agacggagct
     1861 tggccggcgc ggacatgagg ttcatcgtca tgctatggtg tcttccggac tcagcggatt
     1921 acatgattac aactggccgg aagcaacgta tgattacata gaccgatacc atccggatct
     1981 actgattgcc atgtttggtg caaatgacag cctcgctgtg aagaactacg cggacgatgc
     2041 aaagtttact ttctttagtg attcgggatt tcgcgaacgt tatgcgcgca aggtgcgact
     2101 tttcctcagt ctgaatgctc cgagagtgaa aactgtctac tgggtgggac agccaaacct
     2161 ggatcaccac gagttgactc ctaaattccc tgtgctcaat gaaatctatg cgcaagaagt
     2221 ggcgcgagtt cccaatggtg tgttcattcc agtatgggac tttacttcga acgcgggaaa
     2281 atacaaacct gtgatggcgg atgcaaccgg ggtcgtcggt ccactcaaat gggactttgt
     2341 ccactttaca cctcacggcg gtaaggtgct cggggatcat attatagagg cgatctacaa
     2401 ccatcggcgg tctcatgatc gttgaattca gaaaggctgc ttttgttttt atcgtggccg
     2461 gggttacgac tctggcggcg ctgctggcag agaggccaga agtaccggca gaggtcaggt
     2521 cctggctgcc gcgcatcaat gatcttgccc ctgaccttcc cggctttcca tggccgggcg
     2581 cgaaaaattc accttccgat catcgcacgg ccccgggcga agccacttcg cgtgatctga
     2641 agaccggagg cgagacaaag accggagttc cgcagaaaga gaaggtgctt ctcgagaatg
     2701 aaaaggcact ggagggtgtc cttcgcacgc ttggaagaaa ggataggccc gtgcgaatcg
     2761 ttcactcggg cgattcactg atgtgggcag acaacgtatc ttcgcggctc cgtcgtcatc
     2821 ttcaatccag atacggagat ggcggccgcg gaattgtaag cattgtaaat tcaccggatt
     2881 cagtggtgca ggacattcgc attgaaccca ctggattttc gctccaaaaa atttcaggag
     2941 cgtttcctca gggagtggat tctaaacttg gatttttcag cagatcttcg cagtcgatcg
     3001 cagatcctgc gaacgtgcag ttttcatttc cggatggttc aagtccgatt cacaaggcca
     3061 gagtggtctg gtctggaaac ggtacggtca accttgcaag caacggaaaa actctggata
     3121 cagcgacaaa ggtggtagaa ggtgcatgtc gcctgacggt agcagagaca ggcgcagaag
     3181 gtgttcatgc aatcacaatg caggcgtctg gcttgcctgt gatccacgcg gctatcttcg
     3241 agtcttcgtc cggagtttct tattcagctc tagtgaccat gggcatccat caggcctggg
     3301 ttcgcacggt atcggatgaa aacttcatct gcggatggag agccgccgct cctgatctcg
     3361 tgatttttca atttggcctc aatgagtctg cttccattga cacgtactac aatggttttt
     3421 cgcagtccgt ctacgaaact gacctgcgcg catactacac gaggcttcga aaggcattgc
     3481 ctgatgccgc agtgctcatt gtaggtccgc tcgatcgagt aaaatcagcg gacggaggac
     3541 tggttcctgt cgcggcacag acacaggttc gagaagtgca gcgtaagatc gcgcgagagt
     3601 ttggtttcgc gttctatgat actgcggtgg ctctcgggcc ttctcacgtt tctgaattca
     3661 tggcaaaggg tt
//
LOCUS       DAORFF010000405         3671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15629_length_3671_cov_3.091538, whole genome shotgun sequence.
ACCESSION   DAORFF010000405 DAORFF010000000
VERSION     DAORFF010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15629_length_3671_cov_3.091538"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PKK76_14465"
     CDS             <1..423
                     /locus_tag="PKK76_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNL69734.1"
                     /translation="EYRRVLSINPNVPQARLGLDQSLQGQSDQERERAQALEAQGRPF
                     DAIRTYMQALQTLPGNQNAIQGLAALRARQAPQMQELVRHGVQAYDARDYGEAISIFD
                     NVLLVIPGQKEATEYLRLAQKKRAALNILQTKDAEVRQ"
     gene            420..3482
                     /locus_tag="PKK76_14470"
     CDS             420..3482
                     /locus_tag="PKK76_14470"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNL69735.1"
                     /translation="MKSFLSFLLIFTFVSISSLNAEDAVVVVVEFQPFDCPPNPGLAR
                     DLRTAVSGKLTGSGVVVEEAPSAVLNDALAQARQRKAAFVIHGYYNVSGGSVNIYGQI
                     YNPETGVVIDALNAVDESRQIEGLVLPEDETRTPTPRRIEDFSIRLANRILSNPRRTE
                     RRDSINEAILSQRIGKEIQFRLAQENVQSQADQAFKVISERDQIVVAVSKFAQKASEA
                     PADVQVINRSQIRRFGYRNLNEALNSIPQVYSHYVGQNWSADFRGLFINNQIERRVLY
                     LQDGKKLNDYFHFGEFYSDLYTDMDRIDRIEVIKGPGAALYGNNSITGVVNIVTRKPT
                     RENEIETSTDYDAGTGTFAVRALYLSKFSNDLSVTADVSHFEGTGVYRSGQPSWGSTR
                     YYDYRLGSSSQTSASGREDMPYYTGQRIWLATGSQVDNGSPFPNFNFDITYKDFSVKA
                     LYLSKRVSWVDPRTDGGVFGSQTEFGSAINDRIWGVGYAMLEYRPSYLEKYEPSIRVF
                     RQLGINSDFREKGFEGFAGNTAACCGTSATARLASSQYTQFMALNGGSMIKAYASTAY
                     ANGMEFQFTPFRMESSQNFVRKIHALVGGNGAEVNYINYQRIENQRNEVYLYNQGIGD
                     DGRQYGAFGQLTLGLASGTSAIAGMRYDYQKVNRVYRHEYGNAAYTAAENISNPITKT
                     SVPTDTNADFIPFFDPVTGQVRVAQYVRMLQGYRQPFQRKNVVAQDSTPRFALIQSIE
                     QTATTLKLIYSEAFRAVTPQELIRLPSDQGNAESEKVINREVNLIQDLFSGRVSVSLD
                     HFWLRGSTLYAFNAATATFGQSPAWSNTGGSVAISVSPDDLWKFGGSGTSYRLRRASD
                     VSFLDKIDTPSDQALSSPTRLYKANVSRGFFGNKATVAIEYYYNGPIYLMERPPATLQ
                     DVLDSNLELNALPPLPAELDRNSLSGKGNIRNYRIYKVPPSWFYNLTISSNLDSELFF
                     VFSIKNILNRVVLYPLDFDSESFSSPNFAPHQLRGFGREFFLKAGYRF"
BASE COUNT          911 a          902 c          942 g          916 t
ORIGIN      
        1 gaatacaggc gcgtgttgtc catcaatccg aatgtccctc aggcacgtct cggattggat
       61 caatctctgc aagggcagag tgatcaggag cgagagaggg cacaggctct tgaggcgcag
      121 ggaagaccgt tcgatgcaat tcgaacctac atgcaggcac ttcagactct tcccgggaat
      181 cagaatgcga ttcagggtct agcggcgctg agagccaggc aggccccgca aatgcaggaa
      241 cttgtacgac acggcgtgca agcatatgac gctcgcgact acggggaagc gatttcaata
      301 tttgataacg tactgcttgt aattcccggc cagaaagaag ctacagagta tctgcgactt
      361 gcgcagaaga agagagcagc actcaatatt ttgcagacta aagacgccga ggtgcggcaa
      421 tgaagtcgtt cctttctttc ctgctgatat tcacctttgt ctcgatttcg agtttgaacg
      481 cagaagatgc agtcgtcgtg gttgttgaat tccaaccgtt tgattgccca ccaaatcccg
      541 gactcgcacg cgacctgcgc acagctgttt ccggcaagct cactggatct ggagttgtag
      601 tggaagaagc tccgagtgct gttttgaatg atgctcttgc gcaggcgcgt cagcgaaaag
      661 ctgcctttgt aattcacggt tattacaacg tgagtggtgg ctccgtcaac atctatggcc
      721 agatctacaa tccagagaca ggagttgtga tcgacgcgct caatgcggtg gatgaatcgc
      781 gtcagattga agggctcgtg cttccagagg acgagacgcg tactccgact cccaggagaa
      841 ttgaagattt ctcaattcgc cttgcaaatc gcatcctttc aaacccgcgt cgaacggaaa
      901 gaagagatag catcaatgaa gcgattctca gtcagcggat tggaaaagag attcaatttc
      961 gcctggctca ggaaaatgtt cagtctcagg ccgatcaagc gttcaaagta atttccgaac
     1021 gagatcagat tgtggtcgcc gtttcgaaat tcgcacaaaa ggcttctgaa gcaccggcgg
     1081 acgttcaggt aatcaatcgc tctcaaattc gtcgttttgg ataccggaat ctgaatgaag
     1141 ctctaaattc gattccgcag gtttacagtc actatgtggg ccagaactgg tccgctgatt
     1201 tccgcggtct gttcatcaac aaccagatcg aaaggcgtgt gctgtatttg caggatggaa
     1261 agaagttgaa cgactacttt cacttcggtg agttttattc ggatctctat acggatatgg
     1321 atcgcattga tcgcattgaa gtgatcaagg gccctggtgc cgcgctctac ggaaacaact
     1381 ccatcaccgg cgttgtcaat atcgtgacgc gaaagcctac gcgagaaaat gaaatcgaaa
     1441 catccacgga ctacgatgcc ggaacaggga ctttcgccgt gcgcgctctt tacctgtcga
     1501 agttttcaaa tgatctgagc gtgacggcag acgtttctca tttcgaagga actggcgttt
     1561 atcgcagcgg ccaaccgagc tgggggagca cgcgatacta cgactatcga ctgggttcta
     1621 gctcgcagac ttcagcgtca ggcagagaag atatgcctta ctacacggga cagaggatct
     1681 ggttggccac gggttctcaa gtggacaatg gttctccatt tcccaacttc aattttgata
     1741 tcacttacaa agatttttcc gtcaaagccc tctatctttc taaacgcgta agctgggtcg
     1801 atccaagaac agacggaggc gtctttggta gccagacaga gttcggatcc gccatcaacg
     1861 atagaatctg gggagttggt tacgccatgc tggaatatcg tccttcctat ctggaaaaat
     1921 acgaaccttc cattcgcgtg tttcgccagc ttggcatcaa ttcggatttt cgtgagaagg
     1981 gattcgaggg atttgccgga aataccgccg cctgctgcgg cacttctgct accgctcgcc
     2041 ttgcatcatc gcaatacaca cagttcatgg cgctgaacgg aggcagtatg atcaaggcgt
     2101 atgcttccac cgcttacgca aatggaatgg aatttcaatt cacgccgttt cgaatggaat
     2161 ccagccagaa tttcgtgaga aagatccatg cacttgtcgg tggcaacggc gcggaagtaa
     2221 attatatcaa ctatcagcga attgaaaatc aaagaaatga agtctatctc tacaaccagg
     2281 gaatcggtga tgacggtagg caatacggag cattcggtca gctaacgctt ggtctggcaa
     2341 gcggcacgtc agccatcgcc ggcatgcgtt acgactacca gaaagtgaac cgtgtgtaca
     2401 gacatgagta tggaaatgct gcttacacgg ccgcagaaaa tatcagcaat ccaatcacaa
     2461 agaccagcgt gccgacggac acaaatgcgg atttcattcc tttctttgat ccggtcaccg
     2521 gtcaggtgag agtggcccag tacgttcgca tgctccaggg ctatcgccaa ccatttcagc
     2581 gcaagaatgt cgttgcacag gattcgaccc ctcgctttgc cctgattcaa tcgattgagc
     2641 agactgcgac aactctgaag ttgatctatt cagaagcatt tcgcgcggtg acgccgcagg
     2701 agctcattcg tctcccgagc gaccagggaa atgcggagtc cgaaaaagtg atcaatcgcg
     2761 aagtgaattt gatccaggat ttgttctccg gtcgagtgag tgtatcgctc gatcacttct
     2821 ggcttcgcgg ttcgacgtta tacgctttta atgccgccac agctactttc ggacaatctc
     2881 ctgcatggtc taacaccgga ggctccgtgg ccatttctgt ttcgccggat gatctctgga
     2941 aatttggggg aagtggaacc agctacagac tgcgtcgggc cagtgatgtg agttttctgg
     3001 ataagattga tactccatcc gatcaagcgc tcagctctcc gactcgcctg tataaagcga
     3061 acgtatcgcg cggatttttc gggaacaagg caaccgttgc cattgagtac tattacaacg
     3121 gccccatcta cttaatggag agacctcctg ccactttgca ggatgttctg gatagcaatc
     3181 ttgagttgaa cgctttgcct cctctgcctg cagaattgga tcgaaactcc ctgtccggga
     3241 aaggaaatat tcgcaactat cggatctaca aggttccgcc atcgtggttc tacaacttga
     3301 cgatttcttc aaacctggac agcgagttgt tcttcgtgtt ctcaattaag aacatactca
     3361 atcgagtggt tctataccca ctcgattttg atagcgaaag cttcagcagt cctaactttg
     3421 caccgcacca gttgcgcgga ttcggaagag aattcttcct gaaggcaggt tacagatttt
     3481 gagcgcgttt cgcatgcttg tcacgagcgt ctttgtgttg tttctctctg gctgcggagt
     3541 ttttgcagat gtgggattga acccttccac tgtttccggc gatgaagctc gatctatcct
     3601 tcaggatcgt ctggcagggt tttttgttaa agacatctca gatcagaacg cgacgagtct
     3661 tggcctggat t
//
LOCUS       DAORFF010000406         3668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15648_length_3668_cov_3.439801, whole genome shotgun sequence.
ACCESSION   DAORFF010000406 DAORFF010000000
VERSION     DAORFF010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15648_length_3668_cov_3.439801"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKK76_14475"
     CDS             <1..226
                     /locus_tag="PKK76_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69736.1"
                     /translation="VLFEICFKDKCHFIEQFEKTHTCDVWQGQTEKRVRKLQTFRYKR
                     KCDLTLADHRPQLNVDDAVYFIVDSELQRQ"
     gene            310..672
                     /locus_tag="PKK76_14480"
     CDS             310..672
                     /locus_tag="PKK76_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69737.1"
                     /translation="MKFVKAVLMVALVAMASLSLVQCAPKSPSVAKVEKSLDDLKAGI
                     EANGTDCAKIAAAIKTPASDLTAALKEVKEKNEKLPATTQVKFAGLVSSFQKIGACSK
                     TPEMQAVFESLVASATAQ"
     gene            complement(680..1954)
                     /locus_tag="PKK76_14485"
     CDS             complement(680..1954)
                     /locus_tag="PKK76_14485"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL69738.1"
                     /translation="MAKLERFWDWLTDRARVGHLSRDEIRAFAILNMICLMAMVSIVT
                     VTTLILVAAGPLRQTRFDVVQNAISLVLYGLCWILVRRGNAVISSVILSLFGNLQITT
                     LVLYYGFGGGIQWYYGVLMAAPLVTIPGKRQPLRMVLASLPLILFLWTHYEFRIRGRM
                     PLAEADPGVVIFFFSNSLSSLLILGVMVAYFRYAAVRAESELEVERRRSDALLLNILP
                     ESIAGRLKLGEKTIADRFENVTVLFADLVHFTKMSAEIPPEEIVRILDEIFSSFDEAA
                     ARLGLEKVKTIGDAYMAIAGAPEEKPDHAHRAVLFGLEMLSILGVISDRLGRKLDLRI
                     GIHTGPVVGGVIGRDKIAYDFWGDTVNTASRMESHGEAGSIHISEETRREIEGGFVTR
                     ERGRMEIKGKGVLQTYFVEARQPAGDGTTAGS"
     gene            complement(1941..2477)
                     /locus_tag="PKK76_14490"
     CDS             complement(1941..2477)
                     /locus_tag="PKK76_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69739.1"
                     /translation="MKFPGNLTKCFDKDLYLMFLDGFEFVPDFDSEDFTAWFRTPATD
                     HRNYLCRFAQEGTGGLFCFWKKDGGDDYSNSPVIYLGSEGERGVAARNFDDFLRVLSS
                     GFILFSLVFAGKQYHFSEAEEDPEEKKENQATFDEFVSWLRAECSIEPAKDVLRMQSD
                     LADLNAEFQSWIEARGKT"
     gene            complement(2482..3096)
                     /locus_tag="PKK76_14495"
     CDS             complement(2482..3096)
                     /locus_tag="PKK76_14495"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNL69740.1"
                     /translation="MLAGCARNFPVAIRDEEIAAFSMLAANLSRAAVPAVICQNRGFC
                     YIFWSASTYNGQPSPVGPAGMDARCNADGNKPNTSQYKALLSGGTRRATTTANLGDGQ
                     LDWVLYASMTYKRPDGTLIGSTNAAKLLIFPLTNGISPSAGSGTWTGMNIDWTDNTDI
                     CTGWTSTGGFGTIGTANATTTGALYQTNAQGCGAVIFNIYCVEQ"
     gene            complement(3309..>3668)
                     /locus_tag="PKK76_14500"
     CDS             complement(3309..>3668)
                     /locus_tag="PKK76_14500"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNL69741.1"
                     /translation="GIWICDFQTAPWLSAALRNGLIELARVRSAMESQSDMKDIVWRY
                     LTGNEFKNRIERMLRTFMSMKQQIERERAQALKWLSERDQQLNLIAEDVNGFRGELEA
                     MLQVSLTGVQSLQLAAG"
BASE COUNT          962 a          990 c          865 g          851 t
ORIGIN      
        1 cgttctattc gaaatctgct tcaaagacaa atgtcatttc atagagcaat ttgaaaaaac
       61 tcacacatgc gacgtctggc agggacaaac agaaaagaga gtgcgaaagc ttcagacgtt
      121 tcgttacaaa agaaagtgtg acctgaccct tgcagatcat cggccccaac tcaacgtaga
      181 cgacgcagtg tatttcattg tagactcgga actgcagcgg caatagccca aaaaaccggg
      241 gcgcaaagag gcgcaattct tcattgacac gcgactgctc cggaagcgat ccatctacag
      301 gagtacaaaa tgaaatttgt gaaagcagta ttgatggtcg cgcttgttgc catggcatcg
      361 ctgagcctgg ttcagtgcgc cccaaaaagt ccttccgttg cgaaagtgga aaaaagcctg
      421 gatgacctca aggcaggaat cgaagcgaat ggcacagact gtgcaaagat tgccgcagcc
      481 atcaagacac ctgcgagcga tctgacagcc gctctcaaag aagtaaaaga gaaaaatgaa
      541 aagctcccgg ccacaactca ggtaaaattc gccggactcg tatcttcttt tcagaagatt
      601 ggcgcttgct ccaagactcc agaaatgcag gccgtgtttg aaagcctcgt agcgtccgca
      661 acggcgcagt gatagcgtgt caactgccgg cggttgtgcc gtcgccggcg ggttgcctgg
      721 cctccacaaa ataagtctgt agtacgccct tccctttgat ctccattcta ccgcgctcgc
      781 gcgttacaaa tccaccttca atctcacgac gagtctcctc gctgatatga atgcttcccg
      841 cctctccgtg cgattccatc ctgctcgcag tattgactgt atctccccag aaatcatagg
      901 cgatcttatc gcggccaatc acaccgccca caaccggtcc ggtatgaatt ccaattctca
      961 aatccagttt ccttcccagc cgatcgctga tgactccgag aatggaaagc atctctagcc
     1021 cgaagagtac cgcgcgatgc gcatgatctg gtttctcttc gggcgctcct gcaatcgcca
     1081 tgtatgcgtc cccgatggtt tttactttct cgagtccaag tcgagcggcc gcttcatcaa
     1141 acgaggaaaa gatctcgtcg agaatccgga cgatttcctc tggcggaatt tccgccgaca
     1201 ttttcgtaaa atgcactaga tccgcaaaca gtacggtcac attctcgaac cgatccgcaa
     1261 ttgttttttc gccgagcttc agtctcccgg caatcgattc tggaagaata ttcaaaagca
     1321 acgcatctga acgtctgcgc tccacctcca gttcagattc tgccctcact gccgcataac
     1381 ggaagtatgc caccataacg cccagaatca agagagaaga aagcgagtta gaaaaaaaga
     1441 agatcacaac acctggatct gcctcggcca ggggcatcct gccgcggatt ctaaattcat
     1501 aatgcgtcca gagaaacaaa atcaaaggaa gagaagcaag aaccattctg agaggttgtc
     1561 tttttcctgg gatggtgacc agcggcgcgg ccatgagcac tccatagtac cattgaattc
     1621 ctccgccgaa tccatagtac agtaccaggg tggtgatctg gagatttcca aatagagaaa
     1681 gaatcacgct cgaaatgact gcatttcctc ttcgcactag aatccagcac aagccataca
     1741 gtacaaggga gattgcgttc tggactacgt caaaccgagt ctgacgcaaa ggacccgctg
     1801 ccacgaggat caaagtggtc acggtgacga tggaaaccat ggccatcaga caaatcatat
     1861 ttagaatcgc gaatgcgcga atttcatcgc gcgaaagatg ccccacacgc gcgcgatcgg
     1921 tcagccagtc ccagaatcgt tcaagttttg ccacgtgctt ctatccagga ctgaaactca
     1981 gcattcaaat ctgcaagatc ggattgcatt cgcaaaacat cctttgccgg ttcgatggaa
     2041 cactcagcac gaagccatga aacgaactca tcaaaggttg cctggttttc ttttttctct
     2101 tccgggtcct cttctgcttc gctgaagtga tattgtttac ccgcaaacac cagggaaaat
     2161 agaatgaaac cggaagatag aacgcgtaag aaatcgtcga agttccgtgc agccacgccc
     2221 ctttcccctt ctgagcccag gtagattaca ggagaatttg aataatcatc tcctccgtcc
     2281 tttttccaga aacagaacaa gccgcccgtt ccttcctgtg caaacctgca gagatagttt
     2341 ctgtgatcgg tagcgggcgt gcgaaaccac gcggtgaagt cctcactgtc aaagtccggc
     2401 acgaattcaa agccgtccag gaacatcaga tacagatcct tatcaaagca cttcgtgagg
     2461 ttgcctggaa atttcaaaac actattgctc cacacaataa atattgaaaa tgaccgcacc
     2521 gcagccctgc gcgttggtct ggtaaagagc gcccgtggtt gttgcgttgg ccgtgccgat
     2581 tgtgccaaag ccgccggtgg atgtccagcc tgtgcagata tctgtattgt ccgtccagtc
     2641 tatgttcatt ccggtccagg ttccagagcc ggcggatggc gaaattccat tggtgagagg
     2701 aaagataagc aattttgccg cattggtaga tccgatcaga gtaccatcgg gccgtttgta
     2761 agtcatgctc gcgtaaagaa cccagtctag ctgaccatct cccagattcg cagttgtggt
     2821 ggctcgcctt gtgccaccgg aaagcagagc tttgtactga cttgtgttgg gcttatttcc
     2881 atccgcattg cacctggcat ccatacctgc aggcccgaca gggctgggtt gtccattgta
     2941 ggtgctcgcg gaccagaata tataacaaaa gccgcgattc tgacagatca cagctggaac
     3001 agcagcgcga gacaaattgg ccgccagcat agaaaaagcg gcgatttcct catcacggat
     3061 tgccaccggg aaattgcgag cacagccggc taacatacac ccgcacagac aaacaccgat
     3121 tcgcattgca tggaacagac gtttgaactg tttcaggatg gtaagatttt tgttcctcaa
     3181 gaaaagtcac gctaccggct cacaatttcg cccggttttc gccttgcgtg caaccttttt
     3241 ttctttggtc aggtgttgtc ctccttcctg cattcatcgc agtctctgaa ataagcggac
     3301 gcgagaactt aacccgctgc cagttgcaga gactgaacac cagtcaacga gacttgaagc
     3361 atggcttcga gctctccgcg aaatccgttc acgtcttctg cgatcaaatt gagttgctgg
     3421 tcgcgttctg aaagccattt caatgcctgt gcgcgctccc gttcaatctg ctgcttcata
     3481 gacatgaatg tccgaagcat tcgctcgatt ctatttttga actcgttgcc ggtaaggtat
     3541 cgccagacaa tgtctttcat atcagattga ctttccattg cagaacgcac gcgagcaagc
     3601 tcaatgaggc cgtttcgcag agcggcactg agccagggag cagtctgaaa atcgcagatc
     3661 cagattcc
//
LOCUS       DAORFF010000407         3661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15702_length_3661_cov_3.297282, whole genome shotgun sequence.
ACCESSION   DAORFF010000407 DAORFF010000000
VERSION     DAORFF010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15702_length_3661_cov_3.297282"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PKK76_14505"
     CDS             complement(<1..437)
                     /locus_tag="PKK76_14505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNL69742.1"
                     /translation="MHVHLFPAPVFRAIWRFFESSSNGLWNIRYRLHGDDHVRHLEEQ
                     GIRKFTGLLYAHKPGMADSLNDFMAESAQRYSGLLPFGTIYAADGDVAARSRRIFEDY
                     GFYGIKLHPFVSREQLDDRGFYPAYEIMEDLGRVLVCHPASAPV"
     gene            546..1625
                     /locus_tag="PKK76_14510"
     CDS             546..1625
                     /locus_tag="PKK76_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNL69743.1"
                     /translation="MEDFILALLSSSAILLSAVIVLALSLRRRTAHLTTLRAEVEKAF
                     GRVPSEPDEMAGIVHRLESDLADFTRTRDSIGRIISFIDGLLQESENQTQALELISPN
                     LQVMSFSMQNIADSAQKQLETIENTGKVFSDFNESTGELFSVISVINDKSIETLSHAK
                     RGTETILGSFQSMDEIHQSLSRIGEIIKTIRNIADKTNLLSLNAAIEAARAGEQGRGF
                     AVVADEVSKLADKSTQSVKQIAGMLRENLETSVQGKEVSDQARSSFDEISGSILDMNE
                     QVQEVNAYVSSEKRQMLDLESQIHTLKNASLEISASIQNQSIQAKELVRSIEILFETT
                     KSVSAGVRSVSQLLEEIRHDPTLSP"
     gene            1713..2924
                     /locus_tag="PKK76_14515"
     CDS             1713..2924
                     /locus_tag="PKK76_14515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNL69744.1"
                     /translation="MKQILVLGGNFAGVTAALEIQRKLGKEAEVTVISPSEKFLYVPS
                     LIWVPFGARKVQDISFQIGPVLSRKGVKFVLDRATKIRPDANIVETAASGEFKYDYLV
                     AATGASLGFDAVEHLDPKDGFVNCIVTPPLAEKAYDAFREIVKNPGPVIVGATQGASC
                     MGAAYEYLFNLDKYLRKAGVRDKVEITWVTPEPFLGHFGIGGVMGGQKMLELFMKMYG
                     IKWHTNATVSKIEKEKLTLGDGTAIPYKLAMLIPPFRGADVMRNSPELVDDKGFVVTN
                     EGYQHIKYKNVYAAGLAVEVIAPFTKCAAPFGVPKTGFPSDVMGKIVAENIKHDMHGT
                     GKFKKLAFGKIPGICIMDAGKKEVWILTNHLFKPRQFEIMIPNMVYNFGKVLLEKYMI
                     WKNRSGLAWMP"
     gene            complement(3230..>3661)
                     /locus_tag="PKK76_14520"
     CDS             complement(3230..>3661)
                     /locus_tag="PKK76_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002721166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNL69745.1"
                     /translation="RIGVVSRSGTLTYEAVGQLTIQGFGQSTCIGIGGDPIIGTKHKA
                     AVELLMNDPETDGIIMIGEIGGTDEEDAAEWIKLNKKKPVVGFIAGQTAPPGKRMGHA
                     GAIISGGKGTASTKMEAMRNAGIVVVESPAEMGAAMKKALG"
BASE COUNT          941 a          962 c          934 g          824 t
ORIGIN      
        1 acgggtgcag aagcgggatg acagaccagc actcgcccca ggtcctccat gatttcgtat
       61 gctggataaa aaccacggtc atcgagctgc tcgcgcgaaa caaaagggtg cagtttgatt
      121 ccataaaacc cgtagtcttc gaagatgcgt ctggatcggg cagctacatc gccatctgca
      181 gcgtagatgg tgccgaatgg caaaaggcca gaataacgct gtgccgattc tgccatgaag
      241 tcattgagag aatccgccat gccaggcttg tgagcataaa gcaaacctgt gaacttccgg
      301 attccctgtt cttccagatg acgcacatgg tcatccccat gcaggcggta gcggatattc
      361 cacagaccat tggaactcga ctcaaaaaac ctccagatcg ccctgaaaac gggggccggg
      421 aagaggtgga catgcatgtc aatcaacgct cgaccctccc tcgaggcgcc tggtcggcaa
      481 accaaaaaaa cccacgagag cgcggtcaga gggctcgaaa tgccgaaaat aagacccagg
      541 gccccatgga agatttcatt ctggcacttc tgagcagttc tgccatactg ctgtctgctg
      601 tgatcgttct ggccctctct ctgaggcgac gaacggccca cctgaccact ctacgcgctg
      661 aggtagagaa agcgttcggc agagtgccat ctgaaccgga tgaaatggca ggcatcgtgc
      721 atcgcctgga gtctgatctg gcagacttca ccaggacgcg cgactccatt ggtagaatca
      781 tttcgttcat tgacgggctc ctccaggagt cagagaacca gacgcaggca cttgaactga
      841 tttctccgaa ccttcaggtg atgagcttct ctatgcagaa cattgcagat tccgctcaga
      901 aacaactcga aaccattgaa aacacaggca aagtattctc agactttaac gaaagcacag
      961 gcgaactctt cagcgtcatc tcagtcatca atgacaaatc gatcgagact ctatcgcatg
     1021 cgaaacgcgg cacagaaacc atcctgggaa gtttccagtc catggacgaa attcaccaga
     1081 gcctttcgag aattggcgaa atcataaaaa caattcgtaa catcgcagat aagaccaacc
     1141 ttctctcgct caatgcagcc atcgaagcgg cgcgcgcagg ggaacagggt cgtggatttg
     1201 cagtggtggc ggacgaggtt tccaagctcg cggacaagag cacgcagtct gtaaagcaga
     1261 tcgcgggcat gctcagagag aacctggaaa cttctgtgca aggaaaggaa gtatccgatc
     1321 aggcaaggtc cagcttcgac gaaatttctg gaagcattct ggatatgaac gaacaggtcc
     1381 aggaagtcaa cgcatacgtg agttccgaga agcgccagat gctggatcta gaaagtcaaa
     1441 tccacacttt gaagaacgca agtctcgaga tttctgcttc cattcaaaat caatctatcc
     1501 aggccaaaga actggttcga tccattgaaa tcctttttga aacaacgaag tccgtgtctg
     1561 caggagtccg gtccgtaagc cagctcctgg aagaaatcag acacgacccc accctttctc
     1621 cgtaaacatt ccatgacaaa aatcatacga aatccgcagt cgcatcgcac gtttgtgctt
     1681 gcataggata ccccataccc tatattgaaa gcatgaaaca aatcctcgta ctgggtggaa
     1741 actttgctgg agtaacagcg gccctcgaaa tccaaagaaa gctaggcaaa gaagccgagg
     1801 tcactgtaat cagcccttct gaaaagtttc tgtatgtccc ttccctcatc tgggttccgt
     1861 ttggagcaag aaaggttcag gacatttcgt ttcaaattgg gccggtgctg agtcgcaaag
     1921 gcgtgaaatt cgtactggat cgcgcaacga aaattcgccc ggacgcaaac attgtagaaa
     1981 cagcggcgtc cggggaattc aagtacgact acctggtcgc ggcaacgggc gcgtctctgg
     2041 gttttgatgc agtggagcat ttggatccaa aagacggatt tgtaaactgc attgtcacac
     2101 ctcctctggc tgagaaagca tacgatgcgt ttcgcgagat cgtgaagaat cctggtcccg
     2161 tcattgtagg agctacgcag ggagccagtt gcatgggcgc cgcttacgaa tatttgttca
     2221 atctggataa gtacctacgc aaggccggag tgcgcgacaa agtggagatc acatgggtga
     2281 ctcctgagcc cttcctcgga catttcggaa tcggcggagt aatgggtgga cagaagatgc
     2341 tcgagctctt catgaaaatg tacggcatca agtggcatac aaatgcaacg gtttccaaaa
     2401 ttgaaaaaga aaagcttaca ctcggcgatg ggacggcgat tccttacaag cttgcgatgt
     2461 tgattcctcc gtttcgcggg gcggacgtta tgcgcaattc acccgaactt gtagacgaca
     2521 aaggtttcgt cgttacaaac gaaggctatc agcatatcaa atataagaat gtgtatgccg
     2581 caggtcttgc tgtagaagtg attgctcctt tcacaaaatg cgccgccccg tttggagtgc
     2641 ccaagaccgg cttcccgtcc gacgtgatgg gcaagatcgt cgcagagaat atcaaacacg
     2701 atatgcatgg aacaggtaag ttcaagaaac ttgcttttgg taagatcccg ggtatttgca
     2761 tcatggatgc gggaaagaaa gaagtgtgga ttctgaccaa tcacctcttc aagccgcgtc
     2821 agtttgagat catgatcccc aacatggtct acaactttgg caaggtcctt cttgagaaat
     2881 acatgatctg gaagaaccgc agcgggctcg cctggatgcc ctgaacagtc ctcccccacc
     2941 ccctgtgggt ggattttggt ggtgggtgca ttcaaagagt gccccacctc ggagcgagcc
     3001 ggccgacctg cggcgccatg ttgcagatcg gcgcgagaag tcaaccgacc cctgtctcac
     3061 ttgcaaagta ccatattctg ggaagtccca tgcaccagcc gcgcccgcgc tacaaactgt
     3121 cggctgctgc tggctgttgc attcttcggt cgcttcggag ggagggcaaa aaaaaagccc
     3181 ccgctttagg agcggaggct ttccgtggtt gcaaggaatg gactttcgtt tagcccagag
     3241 ccttcttcat ggccgcgccc atttctgcgg gagattccac tacaacaatg ccagcgtttc
     3301 tcatcgcttc catttttgtg gacgctgtac ctttcccacc gctgatgatg gcgcctgcat
     3361 gtcccattct ttttccggga ggtgcagtct ggcctgcgat gaagccaacg acaggcttct
     3421 tcttattgag cttgatccat tctgctgcgt cttcttcgtc cgtgcctccg atttcgccga
     3481 tcatgatgat tccatctgtt tccggatcat tcatgagcaa ctctactgcc gccttgtgct
     3541 tcgtgccgat gatcgggtct ccgccaattc caatgcacgt gctctgacca aatccctgaa
     3601 tcgtaagctg gcctactgct tcataggtga gagttccaga acgagacacc acaccgattc
     3661 t
//
LOCUS       DAORFF010000408         3652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15772_length_3652_cov_4.855435, whole genome shotgun sequence.
ACCESSION   DAORFF010000408 DAORFF010000000
VERSION     DAORFF010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15772_length_3652_cov_4.855435"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PKK76_14525"
     CDS             <1..888
                     /locus_tag="PKK76_14525"
                     /inference="COORDINATES: protein motif:HMM:NF028358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     C-terminal domain-containing protein"
                     /protein_id="HNL69746.1"
                     /translation="PLITEGMTYGGASWPMFTLVPDDQACHEKKIILLPTGRMTPYPW
                     TPEVLPVHIARIGSLAIVAVPFECTTMCGRRLRDTVGAELKADGVTDVVIAGLADAYA
                     GYVATKEEYSVQHYEGASTHFGPNQLGAMQQEYAKLARAMRNGETVNPGPTPRDLSNK
                     QSSLITGVVFDDVPLFKSFGDVYQDAAASYSRGTVASAIFWGAHPRNNLRTQGTYLEV
                     QRWNGSSWVTVASDWDPETKYEWARDGIANSKVTVKWSIPATAATGTYRLRQYGNWKS
                     GWTGAISAYTGTSRSFTVN"
     gene            938..2020
                     /locus_tag="PKK76_14530"
     CDS             938..2020
                     /locus_tag="PKK76_14530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNL69747.1"
                     /translation="MDSGKLVSVLTIDDSATVIAYLRTLLKERQIMTDGCGSLAEARS
                     ILAQKKFDLIFLDLVLPDGSGIDLLKEIRAKDKTTPILMLTGRGDVKTVVSAMALGAD
                     GFVEKQDLFLDDFMLAVDRAVEHRHGILAREQLEQLKRDMYAMITHDLRNPIGNAGAA
                     ADLLLTMTPDDPDHKQVLSIIRTSIDSALGLMEQYLDYEKINAGFLELHKSKCNLADL
                     VTASVNNVGMVARKRKQELTVDAQAFEAAVDPLRMQQVMENLISNAIKYTQEGGKISV
                     RLYGTNGSIIFEVEDNGFGIPPAELETIFGKFKQLTNGTKLSRGFGLGLMIAHEIVEA
                     HGGTIKAESGGSIAKGSRFTVRLPAV"
     gene            complement(2065..3099)
                     /locus_tag="PKK76_14535"
     CDS             complement(2065..3099)
                     /locus_tag="PKK76_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNL69748.1"
                     /translation="MKVLVTGGCGFLGSHVCELFRKEGWEVVSYDSMTKYELAKTGYG
                     TEKTRNFNWDYLKALGCEMVKADVCDEQAMKDHSSGCNFIVHTAAQPAMTISWEDPRL
                     DFNTNVLGTFNVLDAARKHRIPVVNTSSIHVYGNSINDTLKEGATSYERTPVAIDEKH
                     PVMVGQLSPLHASKMAAEHYVRTFTDMLQTPGASFRFTGIYGPRQFGGEDHGWVANFA
                     IRAVFGLPLRVFGTGKQSRDILYAGDGAQSYLAYFKNPIPGVYNIGGGPEHKISLIEC
                     IAMIGEILGTKPVIEFHPERPGDMRYFICDITEARKFGFNPKVKPREGVEKLIRWVEE
                     NKSVFNITGK"
     gene            complement(3128..>3652)
                     /locus_tag="PKK76_14540"
     CDS             complement(3128..>3652)
                     /locus_tag="PKK76_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNL69749.1"
                     /translation="ELPADQKIYVSLYNSPSNRILSSYRASLVRFTEMHGAALAEKKV
                     KAVYLRTSCPFHCPLMDPIRPLFEADIKRIGFSYPGSALKVPVYSFYDGRNLQGDADI
                     AIGMYLDMAINPLYWDKSMKPVADSSKITTIVDFGPGKTSQRLSEDTLQGLGVEKNVL
                     ACAVPKDLEALLAP"
BASE COUNT          948 a          909 c          929 g          866 t
ORIGIN      
        1 ccattgatta cggaaggcat gacgtacggt ggagcaagct ggcccatgtt cacgctggtt
       61 ccagacgacc aggcatgcca tgaaaaaaag atcattctgc ttccaaccgg tcgcatgacc
      121 ccgtatccct ggactccaga agtactgcct gtgcacattg cacgcatcgg aagtctggcg
      181 attgtcgcag ttccctttga gtgtactacg atgtgcgggc gtagacttcg cgatacggtt
      241 ggcgcagagt tgaaggcaga cggagttaca gacgtagtga ttgccggact tgcagatgca
      301 tacgcaggat atgttgcaac gaaagaggaa tactcagtgc aacactacga aggtgcttct
      361 actcatttcg gaccaaacca gctcggggca atgcagcagg aatacgcgaa actggctcgt
      421 gccatgcgaa acggagaaac agtcaatcct ggccctacac cgcgcgacct gagcaataag
      481 caatcatcgt tgatcacggg cgttgtgttc gatgatgtac cgctgttcaa aagctttggg
      541 gatgtttacc aggacgctgc ggcttcctat tcgcgtggta cggtggccag tgctatattc
      601 tggggcgctc acccacgcaa caatttgcgt actcaaggaa cctacctgga agtacaaaga
      661 tggaacggtt ctagctgggt cacagttgca tccgactggg atccagaaac gaaatacgaa
      721 tgggctcgag atggaattgc gaactcaaaa gtaacagtca aatggtccat cccggcaacg
      781 gcggccacag gtacatatcg cctgcgccag tatggaaact ggaaatcagg ctggacggga
      841 gccattagcg cctacaccgg aacgtctcgc agtttcacag tcaattgagt gcagttagtg
      901 atttacagtt tgccgggttc cgaacagcct ttcgttcgtg gactccggga aactggtttc
      961 agttctgacc attgacgact ctgcgaccgt aattgcctac ctccgtactc tgctcaaaga
     1021 aagacagatc atgacggacg gatgcggcag cctcgcagag gctcgttcca ttcttgctca
     1081 aaagaaattc gatctgatat ttttggattt agttcttccg gatggaagtg ggatcgacct
     1141 tctaaaagag attcgcgcga aagacaaaac cactcccatc ctcatgctca ccgggcgcgg
     1201 agatgtgaag accgtagttt ccgcaatggc tctgggcgca gatggtttcg tggaaaagca
     1261 ggatttgttc ctggatgatt tcatgctggc cgtggatcga gcagtggaac atcgacatgg
     1321 aattcttgca cgcgaacaat tagaacaatt gaagagagac atgtacgcga tgatcaccca
     1381 tgacctgcgc aatccaattg gaaatgcggg tgctgcggca gatcttctgc tcaccatgac
     1441 gccggatgac cccgaccaca aacaagtcct gagcattata cgcacgtcca tcgattccgc
     1501 actcggactg atggaacaat atctagacta tgaaaaaatc aatgccggct ttctcgaact
     1561 acataagagc aaatgcaatc tcgcggattt ggtcacagca agcgtaaaca acgttggaat
     1621 ggttgcgcga aaacgcaaac aggaactgac cgtggacgca caagcgtttg aggcagcagt
     1681 ggatccactg cgaatgcagc aggtgatgga gaatctgatc tccaatgcga tcaaatatac
     1741 tcaagagggt ggtaagattt cagtgcgact gtacgggacc aatggatcca ttatctttga
     1801 agtagaagac aacggctttg gaattccgcc cgcagaattg gaaacgattt ttggaaaatt
     1861 caaacagctt acgaatggaa ctaaactctc cagaggtttt ggcctcgggc taatgatcgc
     1921 acatgaaatt gtagaagctc acggaggaac catcaaagcg gagtctggtg gctcgatcgc
     1981 gaagggctcg cggttcaccg tccgattacc tgctgtttga tgaagagggc tggattcaga
     2041 cggcgacaga atcgccgcca cttctcactt ccctgtgatg ttaaatacgc tcttgttttc
     2101 ttccacccag cgaattagct tttccacacc ttcgcgaggc ttaacctttg gattgaatcc
     2161 aaacttacgt gcttccgtga tgtcgcagat gaagtatcgc atatctccag gacgctctgg
     2221 atgaaattca atcactggct ttgttccaag aatttctccg atcattgcga tgcactcgat
     2281 gagtgaaatc ttgtgttctg gtccgccgcc aatgttgtac acaccaggga taggattttt
     2341 gaaatacgcg agataactct gcgctccgtc gccggcatac aggatgtcgc gcgattgctt
     2401 tccagttcca aacactcgca aaggaagacc aaagaccgcg cgaattgcaa aattcgcaac
     2461 ccagccatga tcttcgccgc caaactgacg aggaccataa atgcctgtaa aacggaagct
     2521 tgccccgggt gtctgaagca tgtcggtaaa agtgcgcacg taatgttctg ctgccatctt
     2581 ggatgcatgg agcggagaaa gttgccctac cataacagga tgcttttcat caatggcaac
     2641 gggtgttctt tcgtaagaag tggcaccctc tttcagagtg tcgttgattg agtttccata
     2701 cacgtgaatg ctcgaagtat tcacaacggg aatcctgtgc tttcttgcag cgtccagaac
     2761 gttgaaagta cccaggacat tggtgttgaa gtccaggcgt ggatcttccc aggagatggt
     2821 catggcaggc tgcgcggctg tatgaacaat gaagttgcat ccggatgaat gatctttcat
     2881 ggcctgctcg tcgcagacgt ccgcctttac catttcgcag ccaagagctt tgaggtagtc
     2941 ccagttaaaa tttctggtct tttcggtgcc gtatccggtc ttcgcaagct cgtattttgt
     3001 catgctatca tagctaacaa cttcccagcc ttctttgcga aaaagctcac acacatgcga
     3061 accaagaaaa ccacatccac cagtaaccag cactttcatc gcataactcc tattagttga
     3121 tcaagaatca gggcgccagc agcgcttcta gatcctttgg aacggcgcat gcaagcacgt
     3181 tcttctccac gccgagcccc tgcagagtat cctctgaaag tcgctggctc gtttttcctg
     3241 gcccgaagtc aacgatcgtg gtgatcttgc tcgagtctgc aaccggcttc atggacttat
     3301 cccagtaaag gggattgatt gccatatcca gatacattcc aatcgcaatg tctgcgtctc
     3361 cctgaagatt tcttccatca tagaaggaat acacaggcac cttgagcgcg ctgcccggat
     3421 aagagaagcc aatgcgcttg atgtccgctt caaacaatgg gcgaattggg tccatgagag
     3481 ggcagtgaaa tgggcaactg gtacgaaggt aaactgcctt cacttttttt tcagcaagag
     3541 ccgcaccgtg catttcagtg aaacgaacga gagaggcacg atacgaagat agaatgcggt
     3601 tggacggcga attgtagaga ctcacataaa tcttctgatc cgctggcagc tc
//
LOCUS       DAORFF010000409         3644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15834_length_3644_cov_3.727779, whole genome shotgun sequence.
ACCESSION   DAORFF010000409 DAORFF010000000
VERSION     DAORFF010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15834_length_3644_cov_3.727779"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..2262)
                     /locus_tag="PKK76_14545"
     CDS             complement(103..2262)
                     /locus_tag="PKK76_14545"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNL69750.1"
                     /translation="MRQAFFCAWIAFGLLGACKSTYPGGIPASADPASALTACSTSGL
                     ETTGVKRVAVFVFDPGGKEEALRMKVLDAYAAGFLRDKRFRPVEREKLGTILREQGLS
                     SAGLLTGEDAAKMGGVLPVDWIVSATVMRSSGKVTVSGRFLDAVNGSILQGFDCPFLV
                     DSIAADQSVQISQNITNSNNTTNSNNTTTSSTITSNTINSNNTTNSNNQSTNINIGVL
                     NVTDPTRIIQKKCDSVHAPVIAALRNLTSPELVERAARLAMKIRFDMECGQIHFLVMD
                     TFERYGFAPPEYGDFLIRTMDGIDDVSDDSRTQSILRYLAATDGRVDQAEWKSGFSAL
                     MRARTGSVTGYLIYLLNHRREGMIAVDRAREVLSAADAGRLGRPVPAKGGQILYSVLS
                     ALKFDSPAGSGPFVSVLSQNSEKYAAYQYERLSFASLVSLVTAYYRYADEDGRTVMRQ
                     SLARFFAGQKNQSDRAHEHLFYDIAYGLEREANGEDGESPDKRNRAARELQSALIDLH
                     DPMCRAFTLYDRPTLSTRVDQIHFLLRNGAACPGLPTVSELISKVEAGDWDEKASAFR
                     TLVEMRGNAKQAEAVAIRYFKIEGMSRSSEIRAYCAQILGYTRTTNPEAHQLLIAGAG
                     DFDSPVQDSSEKALGRLGLLVKPAILRYLMVPRTRQDQVREIRRRMAMVQTLGRMGRD
                     ASDAIPVLNQIAATDVDSYLQRMAKTAIVNIQENRTE"
     gene            complement(2259..2606)
                     /locus_tag="PKK76_14550"
     CDS             complement(2259..2606)
                     /locus_tag="PKK76_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNL69751.1"
                     /translation="MATSDFLPHYGPRLDERELAREKDKARKLRDSPWWRKKKQSGVC
                     YYCRKKFKPADLTMDHLIPLARGGRSVKENLVPCCKDCNNKKRYYIPAEWNDYLKSLR
                     GEEPPPENQEIST"
     gene            complement(2593..3063)
                     /locus_tag="PKK76_14555"
     CDS             complement(2593..3063)
                     /locus_tag="PKK76_14555"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNL69752.1"
                     /translation="MDEQLKEKIFNRTRQMLDANVSQVMTKTVMTCDHADPAAAAARM
                     ILENGFLGVLVMKDGRPSGMITAFDLLRLGYEEVFDPDRDYLKTTVGAVLGDRPLPSV
                     SSGTKLWELLNMMIEQNLRTVAVVDDETIKGVVSMVDLAKWYRNTHQEIRTGHL"
     gene            complement(3065..>3644)
                     /locus_tag="PKK76_14560"
     CDS             complement(3065..>3644)
                     /locus_tag="PKK76_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69753.1"
                     /translation="AFVIQNEILKAGHRIPRTMSHNFWHQSPLFKRWSFKLGYFPQDF
                     KMAVRILRKNKVMLLFPEAEAGNFKPSTQMYQLQPFNPGFISLSIITGKPIVPACIIG
                     AEETHLNLGTMDWTEKYIGAKVPIPLNLIPLPVQWKIIFLKPVSLEKYGKREAKNIRF
                     LEEAAGNIRMRIQNRIHKELVHRGVFKFYQDG"
BASE COUNT          868 a          951 c          876 g          949 t
ORIGIN      
        1 gacctgattg aagcaaaagt aggattccat tttgaacggg cggcgagcgg ccatacagtg
       61 aacgattgga ttgagcaaat caagaaatag aaccgttagg cgctattcag ttctgttttc
      121 ctgaatattg acaatcgcag tctttgccat tctctggaga tacgagtcca catccgtggc
      181 cgcaatttga ttcaaaacgg gaatggcatc acttgcgtct ctgcccattc ttcccagtgt
      241 ctgcaccatg gccatcctcc tcctgatttc gcgcacctga tcctgtctgg ttcgaggaac
      301 catcaagtat cgaagaatgg caggcttgac gagcaacccg agtcgaccca gggctttttc
      361 agaggagtcc tgaacgggag aatcaaaatc gcctgcgccg gcaatcagta gctggtgtgc
      421 ctctggattc gttgttcggg tatagccaag aatttgtgcg cagtaggctc gaatttcact
      481 ggatcgactc atgccttcta tcttgaagta acgaatggcc actgcttctg cctgcttcgc
      541 gttgcctcgc atttccacga gagtccgaaa tgcagaagcc ttttcatccc agtcccccgc
      601 ctccaccttg ctgatcagtt cagagacagt tgggagtccc ggacaagcag cgccatttcg
      661 cagcaagaaa tgaatttgat ccacccgggt ggagagagtt gggcgatcat acagtgtgaa
      721 agcccggcac ataggatcat gcaagtcgat gagagcactt tgcagttccc tggcggcacg
      781 gtttctctta tcaggactct ctccgtcttc tccattcgct tcgcgttcca ggccatacgc
      841 gatgtcatag aagagatgct catgcgctcg atctgattga tttttctgtc ctgcaaaaaa
      901 tcgagccagc gattgccgca taacggttct accatcttca tctgcgtagc gatagtaagc
      961 agtcaccaga gacacaagcg atgcgaaact cagtctctcg tactggtagg cagcatattt
     1021 ttctgagttt tgactgagaa cagacacaaa gggtccggat ccggcagggc tatcaaactt
     1081 caaagcagat aagacagaat aaagaatctg acctcccttt gcgggcacag gccttcccag
     1141 cctccctgca tctgccgcgg agagcacctc tcgcgcacga tccactgcga tcattccctc
     1201 acgccgatgg tttagtaaat agatcaaata cccggtcacg ctaccagttc gcgctcgcat
     1261 gagagcagaa aagcctgact tccattccgc ctgatcgacc ctgccatccg tggcggccag
     1321 gtagcgaagg atcgattgag tgcgagaatc gtcggaaacg tcgtctatcc cgtccatcgt
     1381 gcgaatcaaa aaatctccgt attccggcgg ggcaaatcca taacgctcaa acgtatccat
     1441 caccaggaaa tgaatctgac cgcattccat atcaaaacgg atcttcatgg ccagacgcgc
     1501 tgccctctcc acaagctcag gagatgtaag attgcgaaga gctgcaatca caggcgcgtg
     1561 cactgaatca catttcttct gaatgattcg agtgggatcc gttacattga gaacacctat
     1621 gttgatgttc gtactctgat tgttgctgtt tgtggtgttg ttgctattga ttgtattgct
     1681 ggtaatcgtg ctactcgtag tcgtgttatt gctgttagtt gtattattgc tatttgtgat
     1741 gttctgcgaa atctgaacgc tctgatctgc cgctatggaa tccactagaa atgggcaatc
     1801 aaacccctgc agaatagaac cgttcaccgc gtcgaggaat ctgccactca cagtaacttt
     1861 gccggaagat cgcataacgg tagcagaaac aatccagtct actggcaaca ccccgcccat
     1921 ctttgctgca tcctccccgg tcaataaccc ggctgaagat aaaccctgtt cgcgaagaat
     1981 cgtcccgagc ttctctcttt ccacaggacg aaaccttttg tcccgtaaga atccggcagc
     2041 atatgcgtcc agaaccttca tgcgcaaggc ttcttctttt ccgcctggat cgaacacaaa
     2101 cacagcgact ctctttacac cagtggtctc aaggcctgac gtgctacagg cagtaagcgc
     2161 ggaagccggg tctgcactgg ccggaatgcc accgggataa gtggatttgc aggcacctaa
     2221 cagcccaaat gcgatccatg cgcaaaagaa agcctgcctc aagtagaaat ctcctggttc
     2281 tcggggggcg gctcttctcc tcgaaggctt ttgaggtaat cgttccattc agcgggaatg
     2341 taatatcgtt ttttgttgtt acagtctttg caacatggaa ccaggttttc tttgaccgat
     2401 ctgccgccgc gagcgagcgg aatcaaatgg tccatggtca gatcagcggg cttgaatttc
     2461 tttctgcaat agtagcagac accggattgt tttttcttcc tccaccaggg gctatcgcgc
     2521 aatttgcgcg ctttgtcttt ctctcgggcc agttcccttt cgtcgagacg tggcccataa
     2581 tgaggaagaa aatcagaggt ggccagtacg gatctcctgg tgcgtatttc tataccactt
     2641 cgccagatcc accatggaaa caacaccttt gattgtctcg tcgtccacaa cagcaactgt
     2701 gcgtaggttc tgttctatca tcatgttgag caattcccat agtttggttc cggaagacac
     2761 agacggaagt ggccgatctc ccaggaccgc acccaccgtt gtctttagat aatcgcgatc
     2821 tggatcgaac acttcttcat agccgagcct gagtaaatca aatgcagtga tcatgccaga
     2881 gggtcttccg tctttcatga ccagtacgcc cagaaatccg ttctccagaa tcatgcgcgc
     2941 cgcggccgca gctggatccg catggtcaca ggtcatgact gtttttgtca ttacctgact
     3001 gacattcgcg tcgagcatct gtcttgtgcg attgaagatc ttctctttca gctgttcgtc
     3061 catatcagcc atcctggtag aatttgaaaa cgccgcgatg aacgagttcc ttatgaattc
     3121 tgttctgaat gcgcatgcga atgttgcctg ccgcttcttc caggaagcgg atgttttttg
     3181 cttctcgctt gccgtacttc tcaagggata cgggcttcag gaaaataatc ttccattgca
     3241 cggggagagg aatgaggttc agcgggatcg gaacttttgc accaatgtat ttttcagtcc
     3301 agtccatggt gccaagattc aaatgcgttt cttccgcgcc gatgatgcat gccgggacaa
     3361 ttggttttcc agtaatgatg gaaagcgaaa tgaagccggg attgaatggt tgcagttgat
     3421 acatctgcgt ggacggtttg aaattccccg cttctgcttc tggaaacaaa agcatcactt
     3481 tgttctttcg aagaatgcga actgccattt tgaaatcttg cggaaaatag ccgagcttga
     3541 aactccagcg cttgaacaga ggactctggt gccagaaatt gtgactcatc gtgcgcggaa
     3601 ttctatgtcc cgcctttagg atttcatttt gaattacgaa tgca
//
LOCUS       DAORFF010000410         3643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15843_length_3643_cov_3.554069, whole genome shotgun sequence.
ACCESSION   DAORFF010000410 DAORFF010000000
VERSION     DAORFF010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15843_length_3643_cov_3.554069"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..474
                     /locus_tag="PKK76_14565"
     CDS             43..474
                     /locus_tag="PKK76_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004505584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNL69754.1"
                     /translation="MQKFIKKILVPVDGSDSSKKALEQAIAVAEAANATITVLEVVED
                     FGPLPGYYEAAPKGEDRVKWISEQRFEKVHPPLDNTKVKWDRKIVEGYPAYEICKVAQ
                     EGSYDLIIIGSRGLNAVTRFLIGSVSDRVVHHAPCTVAVVR"
     gene            477..1127
                     /locus_tag="PKK76_14570"
     CDS             477..1127
                     /locus_tag="PKK76_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNL69755.1"
                     /translation="MQDQRSFDEFVEALLGCRKCSTVVGPPVCGSVRSSKIIAIGQAP
                     GPHEARFGRPFAYTAGRTLFKWMASIGISEDVYRANVNMSAVLRCFPGKAKSSSAKSA
                     GDRVPSPAEIENCKEHLEFEFTFLKPRLVIPIGKLAIGVTMPEAARSPLADVIGRAFQ
                     VSAFGARFDCIPLPHPSGLNVWNNVEPGKSLLVKALSLIKEHPAAKELIKKSSAAS"
     gene            1168..1590
                     /locus_tag="PKK76_14575"
     CDS             1168..1590
                     /locus_tag="PKK76_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HNL69756.1"
                     /translation="MKKLIICAVLATAFACSKAPEPEAPKVEAPSKRVFFIEPANGAS
                     VSSPVKVKMGVEGMTVEPAGKIVEGTGHFHIIIDAPASEEGKVVPADDSHKHYGKGQK
                     EADLTLKPGKHKLVLQFADGEHKSYGEKLTDTIEITVK"
     gene            complement(1640..2239)
                     /locus_tag="PKK76_14580"
     CDS             complement(1640..2239)
                     /locus_tag="PKK76_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNL69757.1"
                     /translation="MSFRVLTSSTVSKGTTFHTPDHHRHAPVTVSSPALEVMTDLKKT
                     TAITIHPQESLDAAHQKMIHRGVRMLLVVDYNDELVGIITASDTLGEKPIQFVQKNGG
                     QARDLKVSDIMTGQSDIDALSIKDVSNARVGDIIETLKHHHRQHGIVVDNQGPRNEIT
                     LRGIFSLTQIARQLGVQIQTYDVAHNLAELAHFMQKVHP"
     gene            complement(2236..>3643)
                     /gene="serA"
                     /locus_tag="PKK76_14585"
     CDS             complement(2236..>3643)
                     /gene="serA"
                     /locus_tag="PKK76_14585"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNL69758.1"
                     /translation="AAAKNLKLVVRAGVGVDNIDIPEASRHGVVVMNSPGGNSVSTAE
                     HAISLMMSMARNVPQANASTRGGKWEKSKFLGVELTGKTLGVVGLGRIGKEVVKRAKG
                     LRMRVIGYDPFIPPANLANLEIDIVSREEILKQSDFVTVHTPLTETTKDFVSKDNLSQ
                     IKKGIRLINCARGGIYNEDALAQGLSDGTIAAAALDVFVQEPVAADHPLLKLENCIVT
                     PHLGASTGDAEFAVAMDTVEQVIDFFTKGIARYALNFPTMEPETLDFLAPYFKGGEKI
                     GRLLTQFLEGDVNSIEIEYAGEIEKFMTDPVKAAILRGALTPALGDEVNFVNAPFLAK
                     DRGIRVTETSEKTCEGYSSAITVKFSSSKTTAGLKFTALQSKPMVVGFQNLPIEFEPE
                     GILLIVQNRDVPRVVGTLGMYLGDQNVNIAHLELGRDSRGGTARCVITTDELLSADAL
                     EKMKRLENILSVVQVDLR"
BASE COUNT          870 a          889 c          924 g          960 t
ORIGIN      
        1 cgatcatcct gtcctcaagg gctgatgcct gggagtgcat atatgcagaa gtttattaaa
       61 aagattcttg ttccagtaga tggatcggac agttcaaaga aagctctgga gcaggcgatc
      121 gctgtggcag aggctgcgaa tgcaacgatc accgttctag aagtagtaga agacttcgga
      181 ccgctgcccg gttactacga agccgcgcct aaaggcgaag acagggtgaa atggatttca
      241 gaacagcgct ttgagaaagt gcatcctcct ctggacaata cgaaagtgaa gtgggaccgt
      301 aagatcgtgg aaggttatcc tgcgtatgaa atctgcaagg tggcgcagga agggtcttac
      361 gatttgatca tcataggcag tcgtggtctc aatgcagtca ctcgcttctt gattggatcc
      421 gtttctgatc gcgtcgttca tcatgctccc tgcactgttg cagtagttcg ctgaccatgc
      481 aggaccagcg gagcttcgat gagttcgtcg aggctctgct tggatgtcgc aaatgcagca
      541 ctgttgtggg tccacctgtc tgcggatccg ttcggagttc taaaatcata gccattggtc
      601 aggcacctgg accgcacgaa gcaagattcg ggaggccttt cgcgtacact gcaggccgca
      661 ctctatttaa gtggatggcc tccatcggaa tcagtgaaga tgtttatcgc gcgaatgtga
      721 atatgagtgc ggttcttcgc tgctttccag gaaaagcaaa atctagttcc gccaaaagcg
      781 cgggagatcg cgttccttct cctgctgaaa tagaaaactg taaggaacat ctggaatttg
      841 aatttacttt cctaaaaccg cgactcgtca ttcccattgg caagcttgcc attggtgtga
      901 ccatgcctga ggcggctaga tctccgcttg cagatgtgat tggtcgggcg tttcaagtgt
      961 ctgcattcgg cgcgcgattt gactgcattc cactgccgca tccctccggg ctcaatgtct
     1021 ggaacaatgt ggagcccgga aagtctctcc tggtgaaagc tctttctcta ataaaagaac
     1081 atccggctgc gaaagaattg atcaaaaaaa gctctgcggc ttcatgaatc agttgccccc
     1141 atcctggatg agtgcgaatc atgatccatg aaaaaattga tcatctgtgc agtgcttgca
     1201 acggcattcg catgcagcaa ggcacccgaa ccggaagcgc caaaagtaga agcgccttcc
     1261 aaacgcgtat tctttattga gccagcaaac ggcgcgtctg tttcttctcc agtcaaagta
     1321 aagatgggag tagaaggaat gaccgtagag cccgcaggca agatcgtaga aggcacaggg
     1381 cactttcaca tcatcattga tgcaccagct tcagaagaag ggaaggtagt gccggcggat
     1441 gattcgcaca aacactatgg caaaggccag aaggaagcgg atctgactct caagccgggc
     1501 aaacacaaac tggttcttca gtttgcagac ggtgagcaca aatcctatgg cgaaaaattg
     1561 acggacacca tcgagatcac agtgaagtag ttcacattct tctcagatgg aaaggcggcc
     1621 tttggccgcc attttgtttt taaggatgca ctttttgcat aaagtgagcg agttcggcca
     1681 ggttgtgcgc cacatcgtag gtctggattt gaacgcccag ctgtcgcgca atctgagtca
     1741 gtgagaagat accgcgcaga gtgatttcat tgcgtgggcc ctggttatcc accacgattc
     1801 catgctgtct gtggtgatgc ttgagtgttt cgatgatgtc tccgactctc gcattgctca
     1861 catctttgat gctcagagcg tctatgtctg actggcccgt catgatatcg gatactttta
     1921 gatctcttgc ctgtccgccg ttcttttgaa caaactggat tggcttttcg cccagagtat
     1981 ccgaagcggt gatgattccc acgagctcat cgttgtagtc aacaaccaga agcatgcgaa
     2041 caccgcgatg aatcatcttc tggtgagctg catccagcga ttcctgtgga tgaatggtga
     2101 tggctgttgt cttttttaag tcagtcatca cctccagcgc cggagaactt acagttacag
     2161 gcgcgtgcct gtgatgatcg ggcgtgtgaa atgtagttcc tttagaaaca gtggaagaag
     2221 tcagtacgcg aaagctcatc gtaggtccac ctgaacgacg ctcaaaatat tctcaagtct
     2281 tttcatcttc tcgagggcat ctgcggacag caattcgtct gttgtaatca cgcatctcgc
     2341 ggtgccccct cttgaatcgc ggccaagttc gaggtgagca atatttacgt tttgatctcc
     2401 cagatacatg cccagagtgc ccactacgcg cggtacatct ctgttttgca cgatcaaaag
     2461 gatgccttcc ggttcaaatt cgatgggcag gttttgaaaa cctactacca ttggcttact
     2521 ctgcagtgcc gtgaatttca gaccggccgt tgtcttgctg gatgaaaatt tcacggtgat
     2581 cgcgctggaa taaccttcgc atgtcttttc gctggtttca gtgactcgaa tgccgcggtc
     2641 ttttgccagg aaaggtgcgt ttacaaaatt cacttcatct ccgagcgcag gcgtgagtgc
     2701 tccgcgaaga atggcagcct tcacaggatc ggtcatgaat ttttcaattt cgcctgcgta
     2761 ttcgatctcg atggaattta catccccctc aaggaattga gtgagaaggc gaccaatttt
     2821 ttctccgcct ttgaaatatg gtgccagaaa atccagcgtc tctggttcca ttgtggggaa
     2881 gttcaaagcg tatcgtgcaa ttccttttgt gaagaaatca atcacctgtt ctactgtgtc
     2941 catggcgact gcaaactcgg catctcctgt ggacgctccg aggtgaggtg ttactatgca
     3001 gttctcaagc ttcaagagag gatggtctgc agcaacaggc tcctgtacaa acacatcgag
     3061 tgcggcggcc gcaatcgttc catctgaaag gccctgagcg agcgcatctt cgttgtaaat
     3121 cccgccgcgc gcacaattga tgagacggat tcctttcttg atctgagaaa ggttatcttt
     3181 acttacgaaa tcctttgttg tttccgtgag aggtgtgtgt actgttacaa agtctgattg
     3241 tttgagtatc tcttcgcgag atacaatgtc aatttccagg ttggccagat ttgcaggtgg
     3301 aatgaaagga tcatagccaa tgactctcat gcgaagtccc ttcgcgcgct ttacaacttc
     3361 tttgccaatt ctgccaagcc ctacaactcc gagagttttt ccagtcagct ctactccgag
     3421 gaatttgctt ttttcccatt tgccgccgcg agtggatgca tttgcctgtg ggacgtttct
     3481 cgccatgctc atcatcaaac tgatggcatg ttccgctgtg gaaactgagt tacctccggg
     3541 agaattcatc acaaccacac cgtgacgcga agcttctgga atatcaatgt tgtctacgcc
     3601 tacaccggcg cgaactacaa gcttcaaatt ctttgccgcg gcc
//
LOCUS       DAORFF010000411         3630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15927_length_3630_cov_3.448671, whole genome shotgun sequence.
ACCESSION   DAORFF010000411 DAORFF010000000
VERSION     DAORFF010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15927_length_3630_cov_3.448671"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PKK76_14590"
     CDS             complement(<1..599)
                     /locus_tag="PKK76_14590"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNL69759.1"
                     /translation="MKTLRKRPLIALVGNPNTGKTTVFNALCGTRQHVGNYPGVTVEK
                     KIGTAVLAGASFDVLDLPGLYSLKAAAPDEQLASDALMGRIEGEDRPDLIVYVADATN
                     LKRNLLVYSQLVELNIPVIIALTMTDLLSETGVDLNMSALEKRLKIPIVPVIGKDLET
                     VEVLKSRITSALKEKPHAQADLVYPRKLEKVVSVLNDRL"
     gene            complement(596..835)
                     /locus_tag="PKK76_14595"
     CDS             complement(596..835)
                     /locus_tag="PKK76_14595"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNL69760.1"
                     /translation="MIPLSDLPIGQSGKIARVAGSSETFQRLRDLGLIEGEVVRMLKR
                     APLGDPIEVRIMNYNLCLRLSEAREIQVKRNQSEK"
     gene            complement(832..996)
                     /locus_tag="PKK76_14600"
     CDS             complement(832..996)
                     /locus_tag="PKK76_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69761.1"
                     /translation="MEVVVVTLLISLAGAYLIWKAAAFFRRKPAGAGPAIHQDCSGCS
                     CSAGDCRKDA"
     gene            1128..1865
                     /locus_tag="PKK76_14605"
     CDS             1128..1865
                     /locus_tag="PKK76_14605"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNL69762.1"
                     /translation="MTWILLPLVGIASGIVAGILGVGGGTILVPAMVFLGIEAHSAIG
                     TSTLAMIFTSLSGTIRNDAKGGLQVAAVIQIGIASLLGAQGGAFLTTLLKTRTLEILF
                     AILLIFITISGLRKTNIEKRSPTKWLVAGRALTGLTGGFLSGLFGIGGGIVMVPLQIL
                     LLGEDLITAVKTSLGVIVITALGATIAHAMHGQVLVLEGITLGAGGVIGAQIGTRMLP
                     ALPQSTVKIIFAILATILASALIFWGK"
     gene            complement(1868..2911)
                     /locus_tag="PKK76_14610"
     CDS             complement(1868..2911)
                     /locus_tag="PKK76_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69763.1"
                     /translation="MMRWLCLISFLCLASTLSAQVSPVPVSDYAFRPDNDEGYIQAWN
                     VDVRTPNAFLYVTLLVSNVGPGDLNNGVSVLVVKPGSSRIWTAEYSVRSLQATPGQFG
                     HRSGLSRIHRDEKGRIILHSEIPGGSPSPKESLNLDLVLTPVDGGLRISGGLLEPEKG
                     SFLRADLPVIAARAEGTLTIGGVQEPLSGIAGLDAIWSNKSPHTYAKRFLLIRSFSEA
                     PGIFIGGFMGKEENFLLRFAVAEKGKVKSSGNVKSLEVNASAIDPFSGYSIPTSVTYG
                     LEDGCKITEVRKGFAGGFDVLGSISTVLRWILRVFFARPYVMHFTSQMTYICNGAKDI
                     SAEAQASYYLINR"
     gene            complement(2911..3375)
                     /locus_tag="PKK76_14615"
     CDS             complement(2911..3375)
                     /locus_tag="PKK76_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69764.1"
                     /translation="MAISDPAVSAPGKILMQIAKKALIFILLGINSYCRSYPEPDEMQ
                     EYYFYGKYTQLHGLARVPARTIDKEKATVTTLGERRNTCREEAIRDAQARFLALTLVQ
                     PLDASEWHRRLNMGAKGEWWGCFERARVRNLFFEYPDYCRVVLVIPCEPGDY"
     gene            3368..>3630
                     /locus_tag="PKK76_14620"
     CDS             3368..>3630
                     /locus_tag="PKK76_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69765.1"
                     /translation="MAILPFASTGANESLAFTEQDLPNLLAGATHFIFESTREHRLQD
                     QAQTEKAVKSSGWTADQPLDSAALRSLCRTTSASRLFAGNAQFL"
BASE COUNT          861 a          986 c          916 g          867 t
ORIGIN      
        1 ttgagtcgat catttagaac agaaactact ttctcaagtt tgcggggata aactagatct
       61 gcctgtgcat gcggtttctc tttgagcgcg gacgttatgc gcgatttaag aacctcgact
      121 gtctcaaggt cctttcctat gacagggacg ataggaatct tgagtctctt ttccagtgca
      181 ctcatattca gatccactcc cgtctcggag agcaaatcgg tcatggtcaa tgcgatgatg
      241 acggggatgt tcagttcgac gagctggctg taaacgagta aattgcgttt gagattggtc
      301 gcatctgcaa catagacaat gagatcaggc ctgtcttccc cttcaattct acccatgagc
      361 gcgtctgacg caagctgttc atcaggcgcg gccgctttca gactgtacag acccggaaga
      421 tcgagcacat cgaacgatgc accggcaagc acagccgttc cgattttttt ttccactgtg
      481 acgccgggat aattgcccac gtgctgtctc gtgccgcaca gtgcattgaa tacagtcgtc
      541 ttaccggtgt tagggtttcc tacaagggcg atgagcggtc tcttgcgcaa cgttttcatt
      601 tttcactctg atttcttttt acctggattt cgcgcgcctc gctaagacga agacacaaat
      661 tgtaattcat gatccgcact tcaataggat ctccaagcgg cgcgcgtttg agcatgcgaa
      721 ctacttcgcc ttcgatgaga cccaagtcgc ggagtctttg gaatgtttcg ctcgaacccg
      781 ccacacgggc gattttgccg gactggccaa taggaaggtc agaaagtgga atcatgcgtc
      841 tttcctgcaa tctccggcgc tacaggagca gccagagcaa tcctgatgga tggcaggacc
      901 tgcgcccgcc ggttttctgc gaaagaaggc agcagccttc cagatcaaat acgcgcccgc
      961 cagagagatc aacagtgtga ccacaactac ttccatgttt tcctcttcat gccatcactc
     1021 acggcccaca acaatcgtcc attcgtttct gagacccagt ctcaaaatcc agaccagatc
     1081 gccaggaatt gaagtatggc gcacaggcgc ccggtgcagc ctgggccatg acctggatcc
     1141 tcttgccgct cgttggcatt gcatccggaa tcgtggccgg cattctgggc gtcggtggcg
     1201 gcacgattct agttcctgcg atggtgtttc ttggaattga ggcacacagt gccattggaa
     1261 caagcaccct tgccatgatc ttcacatcgc tttccgggac aattcgcaat gatgcgaagg
     1321 gcgggttgca ggtcgcagcc gtaatccaga tcgggatcgc ttctctactc ggggcgcagg
     1381 gcggagcgtt cctcactaca ttactcaaaa cacgcacgct ggaaatactg ttcgcgatct
     1441 tgctcatctt cattacaatc tctggactgc gaaagacaaa catagaaaag cgctcaccca
     1501 cgaagtggct cgttgcggga agagctctga cgggactgac gggtggtttc ctctccggtc
     1561 ttttcggaat tggaggcgga atcgtgatgg tgccattgca aattttactt ctcggagaag
     1621 atctcatcac agcggtcaag acaagtctgg gagtcatcgt catcactgct ctgggcgcaa
     1681 cgatcgcgca cgcgatgcac ggtcaggttc ttgttctcga agggatcact ctgggcgcgg
     1741 gaggagtcat tggtgcccaa atcggaacga gaatgctccc ggcattgcca caaagcactg
     1801 tgaagatcat tttcgccatt ctcgcgacta tactcgcgtc tgcccttatc ttctggggaa
     1861 aataagatca gcgattgatt aagtaatacg acgcctgcgc ctctgcagaa atgtctttgg
     1921 ctccgttgca aatgtacgtc atctgcgaag tgaagtgcat tacgtaaggc ctcgcgaaga
     1981 agacacgaag gatccagcgc agcacagtcg aaatgcttcc gagaacatcg aatcctccgg
     2041 caaagccttt gcgcacttcc gtaatcttgc agccatcttc gaggccgtag gtgacagatg
     2101 tgggaataga atatccggag aatggatcga tcgcagatgc attgacttcc agggacttta
     2161 catttccaga cgatttgacc tttccctttt cagccacagc aaacctcaat aagaagtttt
     2221 cctctttgcc catgaatcct ccgatgaaga ttcctggtgc ttctgaaaaa gatcggatca
     2281 gaagaaaccg tttcgcatac gtatgtggcg atttgtttga ccagatggca tccaggccag
     2341 caatgccgct taacggttcc tgaacgccgc ctatcgtcaa tgtgccttct gctcttgcgg
     2401 cgattacagg aagatccgcg cgcaggaaag atcctttttc gggttcgaga agaccacctg
     2461 aaatgcgaag accgccgtcc accggggtca gaactaaatc caggttgagc gactctttag
     2521 gcgacgggct tcctcccggg atctcagagt gtaagatgat tcgacctttc tcgtccctgt
     2581 gaattctgct gagcccgctt ctatgcccga actgaccggg ggttgcctgg agacttcgca
     2641 cggaatattc cgcagtccag attctactgg aacccggttt tactaccaga acagaaacgc
     2701 cgttgtttaa gtcccccggg ccaacattgg aaacaagaag ggtaacatat agaaatgcat
     2761 ttggcgtgcg aacatctaca ttccatgcct gaatgtagcc ttcgtcgtta tccgggcgaa
     2821 atgcatagtc tgaaacagga acgggagaaa cctgcgccga gagagtggac gcaaggcaca
     2881 gaaacgaaat cagacacaac cagcgcatca tcagtaatct ccaggttcgc aggggataac
     2941 caggacaacc ctgcaataat caggatactc gaaaaaaaga tttcgcacac gcgcgcgctc
     3001 aaagcagccc caccactctc ctttcgctcc catgttcagt cgtctatgcc attctgacgc
     3061 atcgagtggc tgaaccagag tcaaagcaag aaaacgtgcc tgggcatccc gaatggcttc
     3121 ctcgcggcac gtatttctcc tttcgcccag ggtagtcacc gtggcctttt ccttgtcgat
     3181 cgtacgggcc ggcactctgg ccagaccgtg aagttgggtg tactttccat agaagtaata
     3241 ttcttgcatc tcatccggtt cgggatagga ccgacagtaa gagttgattc ccagtaagat
     3301 gaaaatcaaa gccttcttcg caatctgcat cagtatcttg cccggcgcac tcacggccgg
     3361 atctgaaatg gccattttgc cttttgcttc caccggagca aacgagtcac tcgccttcac
     3421 ggagcaggat ttgccgaacc tgcttgcggg ggccactcac tttattttcg aatccacgcg
     3481 ggaacatcgc cttcaggacc aggcccagac agaaaaggca gtgaaaagct cagggtggac
     3541 tgcagaccag cctctcgatt cagcggcgct tcgcagtctt tgcagaacca cttccgcatc
     3601 caggctcttc gccgggaacg cccagtttct
//
LOCUS       DAORFF010000412         3627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15946_length_3627_cov_4.575308, whole genome shotgun sequence.
ACCESSION   DAORFF010000412 DAORFF010000000
VERSION     DAORFF010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15946_length_3627_cov_4.575308"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..938
                     /locus_tag="PKK76_14625"
     CDS             207..938
                     /locus_tag="PKK76_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNL69766.1"
                     /translation="MLRFIFVFSLLATGCVSTSTHEREMKTRDDEVSRLKDDVVRLET
                     EKSQLVKDRTALQASVTDMQKALTEARERKAESDKRVADYRSMLSRFRKLIDSGKLKI
                     RMVDGRMVVVLASDVLFASGSADLSPQGKDAVVEVAKVFATMPDRRFQIEGHTDNVPV
                     RTLKYPSNWQLAAERAISVLEVMVQSGVTPDRVSAASFAETRPLKPNDTVEGRSSNRR
                     IEIVIVPDLSSLPGFDELNKLAEEK"
     gene            939..1556
                     /locus_tag="PKK76_14630"
     CDS             939..1556
                     /locus_tag="PKK76_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNL69767.1"
                     /translation="MGVQERKEREKAQRRTSILDAARQIIKEKPYDSIIMEDIANRLE
                     LSRATLYLYFKNKEEIYATLLTLGMRELQAGYDKALKNPSQDPMENLVKCAFEFFTFY
                     MSNHHYFDLLVTNRDDLLRECRPEVRKEFEEAGGAVIKPIAQVYKSGMDSGKFQKQDP
                     DKMAFLLRAVAIGIAVGFREGNLKFPEDILIMRDLVLHGLLGKKS"
     gene            1611..3161
                     /locus_tag="PKK76_14635"
     CDS             1611..3161
                     /locus_tag="PKK76_14635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNL69768.1"
                     /translation="MAKQYDLIVVGGGISGACILWDATLRGMNVLLLEKGDFASGTTQ
                     ATSKLIHGGLRYLKNLEFGLVRESLRERRLLARLAPHAVKPMPFFHPIYKGAKLGRFT
                     LGLALSLYDVLSFDRNRGIPRHLRLPHHRWLSRDEALQAEPGLHPEGLQGAYIYYDYQ
                     NIGPERVCSDFIFSAVERGATARNYAEVKAIQQTLDGYSVTIEDRLTSKKESFEAKSV
                     VNAAGPWADLVDRATSSENSRKIVRSMGIHLVTRRIAGDSTVVLHRKDGSHFFIIPWR
                     EFSLIGTTDSLYEGHPDKFTVPESEIQKLIEDVTEGLNAKLTRADVVSVYGGLRPLVE
                     ESVSVKDSYSASRKTEIEMHADKPGFFTVLGGKYTTSRHLAEKAVDQIAAFLGGTWKK
                     CSTHRTALRTGDFGTQVLLKQKLFLERKHDRKKIETLISRYGLIADRMLSEKNLGVAF
                     TLDNGEVYYKEELETILNTEMVKSLKDLLLRRTGIGTLGISSPLPFAEEHGHVLLTQP
                     EATLRKAG"
     gene            complement(3158..>3627)
                     /locus_tag="PKK76_14640"
     CDS             complement(3158..>3627)
                     /locus_tag="PKK76_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69769.1"
                     /translation="ASGLNPQVWICVCLALASSILSMFFIREKIDPYYLAFMPAITLA
                     LLSIFARRIPIVLSILCLFALPAAVSNVNRYLNEAWNVCPLGEVQTVARALNGKMAET
                     KGASIPFVLPSILREAGELHPAKGLLIEAPPGDSSSRDIFRGKAIRLLEQPAR"
BASE COUNT          974 a          946 c          904 g          803 t
ORIGIN      
        1 cagccccgcc gcctccgcct gcacagtgag gtctccggtc tttcgaccgg aggttcttga
       61 aaccccggct tatctttttt ttcgccgtta ccctgtaaca gacagtctga tcgggcatgt
      121 caagaaaaat cgacccttgc tggaaggcct ggactgcaaa tccaaataac gattgcgcgc
      181 acatgtattt cggccactta cagtcaatgc tccgtttcat ctttgttttc agccttctgg
      241 ccacgggatg cgtttccacc tccacccatg agcgggaaat gaaaacgaga gacgatgaag
      301 tctctcgcct caaggacgac gtggttcgcc tggaaacaga aaagagccag cttgtaaaag
      361 atcgcactgc cctgcaggcc agcgtcacag atatgcaaaa ggcgcttacg gaagcgcgcg
      421 aaaggaaagc ggagtcagat aagagagtcg ccgactatcg ttccatgctc tcgcgctttc
      481 gcaaactcat cgattctggc aaactcaaga tccgaatggt ggacggaaga atggttgtag
      541 tccttgcttc ggatgttctt ttcgcgtccg gatccgcgga cctttcacca cagggcaaag
      601 acgcagtcgt ggaagttgca aaagtatttg caaccatgcc ggaccgcaga ttccaaattg
      661 aaggacatac ggacaacgtt ccagttcgca ctctgaaata tccatccaac tggcagctcg
      721 ccgcggaaag agccatcagt gtgctcgaag tcatggttca atccggtgtt acaccggacc
      781 gagtgagcgc tgcaagcttt gcggaaacaa ggcctcttaa accaaacgac actgtagaag
      841 gcagatcatc gaatcgcaga attgaaattg taattgtgcc ggatctgtcg agccttccag
      901 gtttcgacga actcaataag ctagcagaag agaagtaagt gggcgtacag gaaagaaaag
      961 aaagagaaaa ggcgcagcgc cgtacatcga ttctggatgc tgcaaggcaa atcatcaaag
     1021 aaaaaccgta tgactccatc atcatggagg acattgcgaa tcgtcttgag ctttcgcgcg
     1081 caactctcta tctctatttc aaaaacaaag aagagatcta tgcaacgctt ctcactctgg
     1141 ggatgcgcga actccaggct ggctacgaca aagctctaaa aaatccttcc caggacccca
     1201 tggagaacct ggtaaagtgc gcgtttgaat tcttcacttt ctacatgtcc aatcaccatt
     1261 acttcgacct gctcgtcaca aatagagacg atctgttgcg cgagtgcagg ccggaagtaa
     1321 gaaaagaatt cgaagaggcg ggcggcgctg tcatcaagcc aatcgcccag gtttacaaaa
     1381 gcggaatgga cagcggaaaa ttccaaaagc aggacccgga caagatggct ttccttttgc
     1441 gtgccgtggc cattggaatt gcagtcggat tccgcgaagg aaacctcaaa ttcccggaag
     1501 acatactgat catgcgagac ctggtcctgc acggactcct gggcaagaaa tcctgacaga
     1561 ctgtcatttt ttggcttgac gttctgacca aacactgaca cggtgtcagc atggcaaagc
     1621 aatatgacct catagtcgtt ggcggtggta tttcgggcgc atgcattctc tgggatgcaa
     1681 ctctgcgagg catgaatgtg ctcctactcg agaagggtga ctttgcttct ggaacgaccc
     1741 aggcaacgag caaactcatc cacggtgggc tccggtattt gaaaaacctt gagtttggtc
     1801 tggttcgaga gtccctgaga gaaaggcgtc ttctggcccg tctggcccca catgcggtca
     1861 agcccatgcc attcttccat ccaatctaca aaggtgcgaa gctcggacgc ttcacactcg
     1921 gtcttgcgct ctcattgtat gatgttctct cctttgatag aaaccggggg attccccgcc
     1981 atctgcgtct tccgcaccac agatggctct cccgtgatga ggccctgcag gctgagcctg
     2041 gcttgcaccc ggaaggcttg cagggcgctt acatctacta cgactatcag aacattggac
     2101 cggaaagggt ttgctctgat ttcattttct ccgccgtaga acgaggagcg actgcgcgca
     2161 attatgcaga agtcaaagca attcagcaga ctctggacgg ttacagtgta acaatcgaag
     2221 atcgcctaac gtctaaaaaa gaatcattcg aagcaaaatc tgtagtaaat gctgcgggcc
     2281 catgggcaga cctggtggat cgcgcgacaa gctcagaaaa ctctcgcaag attgttcgtt
     2341 ctatgggaat ccacctggtc actcgccgca ttgcaggcga ttccacggtg gttctgcata
     2401 gaaaagacgg ttctcatttc ttcatcattc cctggagaga attcagtctg atcggcacta
     2461 cagattccct gtacgaaggc catccagaca agttcactgt tccggaatct gaaattcaaa
     2521 agctcattga ggatgtaacg gaaggactaa acgcaaaact cacgcgcgca gatgtagttt
     2581 ccgtctacgg cggtctccgt cctctcgtag aggagtcggt ttcagtaaag gattcgtatt
     2641 ctgcatcgcg caaaactgaa atcgaaatgc acgcagacaa accggggttc ttcacagtcc
     2701 tgggtggtaa atacaccacc agtcgccatc tggcggaaaa agcagtagat caaatcgctg
     2761 cattccttgg cggaacctgg aagaaatgct ctacgcaccg caccgctctt cgcacaggcg
     2821 attttggcac ccaggttttg ctcaaacaaa agctatttct ggaacgcaaa cacgatcgca
     2881 agaaaatcga gactctgatt tccagatacg gactgattgc agatcgcatg ctgtccgaaa
     2941 aaaatctagg agtggccttc accctggata acggcgaagt ctattacaag gaagagctcg
     3001 aaacaattct gaatactgaa atggtaaaaa gcctcaaaga tttgcttctt cgtcgcactg
     3061 gaattggaac acttggaatc tcaagtcctc tcccgttcgc agaagaacac gggcacgtac
     3121 tactaacaca gccagaagct actctccgca aagccggcta acgggccggc tgctcgagca
     3181 gcctgatcgc cttgcctcga aaaatgtccc gactggaaga atcccccgga ggggcttcta
     3241 tcaagagccc ctttgccgga tgcagctctc ctgcctctct tagaatggat ggcaagacaa
     3301 acggaatgga tgctcccttt gtctctgcca tttttccatt cagagcacgc gcgactgtct
     3361 gcacttcccc gagaggacat acgttccacg cctcgttcaa atatctattc acgttactca
     3421 cggccgccgg cagtgcgaaa agacaaagta tagagagaac aattggaatt cgacgtgcga
     3481 aaatggaaag cagtgcaagc gtgatggccg gcataaacgc gaggtagtac ggatcaatct
     3541 tctcgcggat aaaaaacatg gacagaatgg aagaggccag agccaggcag acgcaaatcc
     3601 atacctgtgg attcaggccg gatgctg
//
LOCUS       DAORFF010000413         3627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15949_length_3627_cov_2.958567, whole genome shotgun sequence.
ACCESSION   DAORFF010000413 DAORFF010000000
VERSION     DAORFF010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15949_length_3627_cov_2.958567"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PKK76_14645"
     CDS             <1..1023
                     /locus_tag="PKK76_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNL69770.1"
                     /translation="QRINEILKIEPAVRDQRKRPDIKLEFDEIIGESTQVQAVKRAIA
                     QAAGTNARVFIYGENGSGKELVARAIYNNSRRVGFPFVEVNCAAIPEELIESELFGHE
                     KGSFTGAQEKRIGKFEMAHQGTLFLDEICDMSMATQARVLRALQEQRFTRVGGSENIE
                     VDVRIIAATNIDPEKAIAQGRFREDLYYRLNVIPIHLPALRERTSDVPLLLDHFLRSA
                     ARENGIPIKRFSQGAMEMLQMYAWPGNVRELKNVVERLSIMSQDEEITVDTVTEHLRP
                     PNRGQEQTPIGDLKKARDDFERTYIIQALKQHDKNITRAAKFLGMDRTNLHRKLKSLE
                     IDPDTL"
     gene            1023..1814
                     /locus_tag="PKK76_14650"
     CDS             1023..1814
                     /locus_tag="PKK76_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69771.1"
                     /translation="MSVEIFHRVTLDLASALRDDPEGMATAYAGEPLPEQVPLIYRPP
                     GKKESPQVQSDLSSPGSPGKQPGKPPVREIAKKHLPANFTCELCPDRLFPVRRFQRAG
                     KIPVLFVFFNGSVVPTRVRPDKSNDFILSSKQEDDYFASFLSPYGFALDSFYYQQFPA
                     CHFNPERSSLGDWQRRSDACLVHLKAAVETAQIQHLILTSPSSQFLLGKDESQRLMTS
                     GEEFLFERSGIRLTGHVMRRADPKNEESQARFKSILAAVQKRFHV"
     gene            1807..>3627
                     /gene="priA"
                     /locus_tag="PKK76_14655"
     CDS             1807..>3627
                     /gene="priA"
                     /locus_tag="PKK76_14655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNL69772.1"
                     /translation="MFDRFADVWLGIPLDGPLTYRIPPSMDVTPGSLVRVPLRGEIEK
                     GVVRSIHSSAPGYEVRDILQAGPVVVTEAQMDLAAWVAEEYFSGPGEAIFKMFPDPPT
                     RLSKPASIDRKDTFRPAHDLNEEQAKAYNGIISSRSGTLEERIHLIEGITGSGKTEVY
                     IHLIRAILASGGSAILIVPEISLTVQIQRRLKDVFGDEVAVLHSGMKPKDRWNSYKSV
                     LEGQRRIALGTRSAIFAPVNPGIIIIDEEHDGSYKEHSSPRYDARAVALQIAKKTGSL
                     VVFGSATPRLESRSLKNVHRLKARATGAQMSSAQIIPATESHLSPPLFAAMKEALSKK
                     EKVLLLLNRRGYFPYLYCKSCRKTVQCPKCSVTLPLHRDGNLRCHYCGYSRPDDGKCD
                     RCKSPVKKLGMGTQRMEEVLLSLFPDARIERIDTDNARGEAVQDCISRFLAGEIDILL
                     GTQMIAKGLDSPNVTVVGVLKADQGLAMPDFRSAERTFALLTQVAGRAGRADLPGRVF
                     LEALDPEHPVLIMAQKQDYSHFYETELEDRRAAMYPPFCRLIRLLVRGADESESEKDS
                     VKLGELLAGLPGKSTVVLGPAQAPIYRVNEQYRNHILIKTN"
BASE COUNT          966 a          904 c          923 g          834 t
ORIGIN      
        1 cagagaatca atgaaatact caaaatcgaa ccggctgttc gagatcagag aaagcgtccc
       61 gacatcaaac ttgaattcga cgaaatcata ggagaatcca ctcaggtgca ggcagtcaag
      121 cgcgcgatcg cgcaggcagc cggaaccaac gcacgggtgt tcatttatgg cgaaaacggt
      181 tcaggcaagg agctcgttgc acgcgcgatt tataacaact caagaagagt cgggtttcct
      241 tttgtggaag tgaactgcgc cgctatcccg gaagaactga tcgagagcga actctttggt
      301 cacgagaaag gatcgtttac gggcgcacag gaaaagcgga ttggcaaatt cgagatggct
      361 catcagggta ctcttttcct ggatgagatc tgcgatatgt ccatggccac tcaggctcgc
      421 gtacttcgcg cacttcaaga gcaacgattc accagggtgg gcggatcaga gaacatagag
      481 gtggatgttc gaatcatcgc tgctacaaac attgacccgg agaaggcaat cgcgcagggt
      541 agatttcgag aagatttgta ctacagactc aacgtaattc cgattcatct tcccgcgctt
      601 cgagaaagaa cttcagatgt tccattgttg ctcgatcatt tccttcgctc tgctgccagg
      661 gaaaatggaa ttccgatcaa gagattctcg cagggtgcca tggaaatgct ccagatgtac
      721 gcctggccag gaaatgtgcg cgagctcaag aacgtcgtgg aaagactctc gatcatgagt
      781 caggacgaag agattacggt ggatactgtt acagagcatc tgcgaccacc gaatcgaggg
      841 caggaacaga ctcctattgg cgatttgaaa aaagcacgag acgatttcga acgaacttac
      901 atcattcagg cactcaaaca acacgataag aacatcactc gagcggccaa gtttctggga
      961 atggatcgaa ccaatctaca ccgcaaattg aaatcacttg aaattgaccc ggatactctg
     1021 tgatgagcgt agaaattttc cacagagtca cgctcgactt ggcctctgct ctcagagacg
     1081 atccggaagg catggctact gcgtatgcag gtgagccctt gccggagcag gtgcctctca
     1141 tctacaggcc cccgggcaag aaagaatcgc cccaggttca aagcgattta agttcacccg
     1201 gatctccggg gaaacaaccc ggaaaaccac ctgtacgaga aatcgcaaag aaacaccttc
     1261 ctgcaaattt cacatgtgag ctatgtccgg atcgcctatt tcctgttagg cgattccaac
     1321 gcgcaggcaa gatccccgtt ctctttgttt tctttaatgg aagtgtggtc ccgaccagag
     1381 tgcgtccgga taagagcaat gacttcattc tttcaagcaa gcaggaggat gactatttcg
     1441 cttcctttct ctctccgtat ggctttgcac tcgattcttt ctactatcaa caattccctg
     1501 cctgtcattt caatccggag agaagttccc ttggtgactg gcagagaaga tccgacgcat
     1561 gcctggttca cctcaaagcg gcggtcgaaa ccgctcaaat ccaacatctc atccttacat
     1621 ctccgagctc tcagttcctc ctgggaaagg acgaatctca gcgtctgatg acatcaggag
     1681 aagaattctt gtttgaaaga tctggaatca ggctcaccgg gcacgtgatg cggcgagcgg
     1741 atccaaagaa tgaagaaagc caggcccggt tcaagtccat actcgcggcc gttcaaaagc
     1801 gatttcatgt ttgatcgttt tgcagacgtc tggcttggga ttccactgga cggacctctt
     1861 acttacagaa ttccgccgtc gatggacgtg actcctggct ctctcgtgcg cgtacctctc
     1921 agaggtgaga tcgaaaaagg agtagttcgt agcattcact cttccgcgcc aggctatgaa
     1981 gtgcgcgata tccttcaggc aggccctgtt gtggtgacag aggcacaaat ggatctggca
     2041 gcctgggtgg cagaagagta cttttcaggc cccggagaag ccatcttcaa aatgtttccg
     2101 gatcctccca cgcgcttgtc taagccggca tccatcgatc gtaaggacac ctttcgtcct
     2161 gcgcatgatt tgaatgagga gcaagcaaag gcgtataacg gtattatttc ttctagatcc
     2221 ggcactctgg aagaacgaat tcatctgatc gaaggcatca ctggatccgg taagactgaa
     2281 gtttacattc atctcatccg ggccattctc gcatctggtg gaagtgccat tctaatcgtt
     2341 ccggaaattt ctctgacggt ccaaatccag aggagattga aggatgtatt cggagatgag
     2401 gtggctgttc ttcattcagg aatgaagcca aaagacaggt ggaattccta caaatcggtt
     2461 ttagaaggac agcggcgcat cgcactggga acacgctccg ctatctttgc tcctgtaaat
     2521 cctgggatta tcatcataga tgaggaacat gacggctcct ataaggaaca cagctcgccc
     2581 agatatgacg cgcgcgcggt ggccttgcaa atcgcaaaga aaactggatc tctggttgta
     2641 tttggttctg caacacccag gcttgaatct cgatcattga aaaatgtaca cagattgaaa
     2701 gcacgagcca cgggtgctca gatgtccagt gcgcaaatca ttcctgcgac agaatcgcac
     2761 ctttcaccgc ccctgtttgc tgccatgaaa gaggctcttt ccaaaaagga gaaagtgctc
     2821 cttcttctca atcgcagagg ttactttccc tatctctact gtaagtcgtg tcgaaagaca
     2881 gtgcaatgtc cgaagtgctc tgtcactctg cctctgcata gagacggaaa cctccggtgt
     2941 cactactgcg ggtactcaag gccggatgac ggtaagtgtg atcgatgcaa atctcctgtc
     3001 aaaaaactgg gaatgggcac ccagagaatg gaagaagtgt tgctctctct ttttcctgac
     3061 gcgcgcattg aaagaatcga tacagacaac gcaagaggag aggcggtaca ggattgcatt
     3121 tcgcgcttcc tggcaggaga aatagatatc cttctcggaa ctcagatgat tgccaaagga
     3181 ctcgactcac cgaatgtcac tgtcgtagga gtgctgaaag cagatcaagg cctggccatg
     3241 cctgatttta gaagtgcgga gagaacgttt gcacttttga ctcaggtagc agggagagcg
     3301 gggcgcgcag atctacccgg aagagtcttt ctcgaagcgc tcgatccaga acacccggtc
     3361 ctcatcatgg cgcaaaaaca ggactactct catttctacg aaacggaact tgaggacaga
     3421 agggcagcca tgtatccgcc gttctgcaga ttgattcgcc ttctggtgcg cggtgcagat
     3481 gagtccgaaa gcgaaaaaga ttccgtgaaa ctgggcgagt tactggcagg tcttcctgga
     3541 aaatcaactg tagtgctagg cccggcgcag gcacccatct accgagtcaa cgaacagtac
     3601 agaaatcaca tattgatcaa gacgaat
//
LOCUS       DAORFF010000414         3625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_15962_length_3625_cov_3.877031, whole genome shotgun sequence.
ACCESSION   DAORFF010000414 DAORFF010000000
VERSION     DAORFF010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15962_length_3625_cov_3.877031"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PKK76_14660"
     CDS             <1..527
                     /locus_tag="PKK76_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNL69773.1"
                     /translation="MRGSDPDAALLYLAIMIDAGEDPLFIARRIIIFASEDVGNAAPQ
                     GLQVAVSAFQALERVGMPEGRIILAQAATFLASCPKSNAAYRGIDAALEFIQGKRISV
                     PNHLRNAPTTTHRKEGAGKGYKYPHEYSGHYVEQEYMPDDIRAQFYFPTREGQEVRLL
                     ERLRMLSRKDRKYE"
     gene            524..1441
                     /locus_tag="PKK76_14665"
     CDS             524..1441
                     /locus_tag="PKK76_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69774.1"
                     /translation="MRPQPFLLAIGYLLLAVPVMGEPWTQFKVKKAFISPETYQENKD
                     TFSDPEFGWNVFCENDLIMRAGLYDLLFSPPCKGKIVLYFVERDVVWDDKITPFPVAL
                     QPEKQRLVDGEDEFEIQLSSYNPDSNSGPDSITPRILKDGEVVKDHVSFLDGDRTDTF
                     ACPAGITLLMRSSLSVQTEASALWTDDSQKLSRIDSKDNVRILSPPGFAVSDYTIACA
                     GNTSIMLQTLSKLASEDKGLFRSARQLIDSNAEAQKYCNSNLLEPVCIYRQYMSQKGD
                     RIRLENMRLPPAARAACNQAREDATYLEN"
     gene            complement(1459..2391)
                     /locus_tag="PKK76_14670"
     CDS             complement(1459..2391)
                     /locus_tag="PKK76_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ kinase"
                     /protein_id="HNL69775.1"
                     /translation="MTTVEKAVVVTRPTRLQESVKRYNTKAQAKFYVEKRGQSFQDYE
                     LEEDIYEHSRDHVLKAIPRDLKIQIVDRNFLPNYLFSPKDLIICLGQDGLVVNTAKYL
                     EGQPVIAINPDPDRFDGVLLPFLPDQTEAAVQAVMNGKFQARNLTMARVDMNDGQKLY
                     AFNDLFIGPRSHITARYTIHDGERSERQMSSGILVATPAGSTGWISSLFNQTRGIARF
                     QGDGSPLAGMRLEWEDQKLVYMVREPFASKWSKTEMVAGVIEGTNSISVESHMSESGV
                     IFSDGMESDFIQFNTGSVARIGIAEKSTTLVVKF"
     gene            complement(2388..3452)
                     /locus_tag="PKK76_14675"
     CDS             complement(2388..3452)
                     /locus_tag="PKK76_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNL69776.1"
                     /translation="MLGIRYIKTRPTEYVMLFRGGKLRKHGAGLAFFYYAPSSSVVIL
                     PAESRDIPFIFNEVSNDFQEITIQGQATYRVRDPEKLAALLDFSVDVFSNYTGDGAEK
                     LGVRVTNSVQLAIREKFKEMSLRDALAGASALVQHAAGKLKSSELLSALGIEILDLSI
                     LKIAPTPDMSRALEASAREKLYKEADDAIYERRNSAVEQERRIKENELQTQIAIEEKN
                     RKIREEQMNAEIAVQEKQRAVEEEKMKTVESVERKKAEIEDQKMLSRTALEEKKRKLV
                     EVEVQNNIQYAKARAESMRLELEAVMKLPPDVLEVLMANQMQSRKLISRAMRDLAKNA
                     QKIGTLNITPDLLQNLLKDE"
     gene            complement(3502..3584)
                     /locus_tag="PKK76_14680"
     tRNA            complement(3502..3584)
                     /locus_tag="PKK76_14680"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3548..3550),aa:Tyr,seq:gta)
BASE COUNT          881 a          927 c          878 g          939 t
ORIGIN      
        1 ccatgcgcgg ttcggatccg gacgcggcgc tcctctatct ggcgatcatg atcgatgctg
       61 gtgaggatcc tcttttcatc gctcgcagaa tcatcatatt tgcctcggag gatgtcggaa
      121 atgctgcacc acaagggctg caagtagctg tgtctgcctt tcaagccctg gaaagagtag
      181 gaatgccgga aggcagaatc attcttgcac aggccgcgac atttctcgcc tcgtgtccga
      241 agagcaatgc cgcctatcgt ggaatcgatg cggcccttga gttcattcaa ggaaaaagaa
      301 tctcagttcc aaatcatttg cgaaatgctc ccaccactac acacagaaaa gaaggcgcag
      361 gaaaaggtta caaatatccg cacgaatatt caggtcacta cgtcgaacag gaatacatgc
      421 cggatgacat tcgcgctcaa ttctattttc caacaaggga aggccaggaa gtgaggctct
      481 tagagagact gcgaatgctt tcgcgaaagg acaggaaata cgaatgaggc cgcaaccgtt
      541 tctcctggcc attgggtatc ttctcctggc agtgcccgtc atgggggaac cctggactca
      601 gttcaaagtc aagaaagctt tcattagccc ggaaacatac caggaaaata aagatacttt
      661 cagtgatcct gaattcggat ggaatgtatt ttgcgaaaat gatctcatca tgcgggcggg
      721 tctctacgat ctgctcttca gtcctccgtg caaaggcaag atcgtactgt acttcgttga
      781 gagggacgtg gtctgggatg acaaaattac gccctttcca gtggccttgc aaccggagaa
      841 acaacgactc gttgacgggg aagacgaatt cgaaattcaa ctctcctctt acaatccaga
      901 ttcaaattca ggcccggatt caatcactcc gcgaattctc aaggacggag aagtcgtcaa
      961 agaccatgtg tcttttctgg acggtgatcg cacagacaca ttcgcttgtc ctgccggcat
     1021 aacactgctt atgcgaagct ctctgtctgt tcagacagaa gcgtccgctc tctggacaga
     1081 cgattcacag aaactttccc gaatagactc gaaggacaac gtgcgtattt tgagccctcc
     1141 tggatttgcg gtgtccgact acaccattgc atgcgctgga aatacatcga ttatgcttca
     1201 gactcttagc aaacttgcgt cggaagacaa agggttgttt cgctctgcca gacagctcat
     1261 cgattcaaac gcagaggctc aaaaatactg caattccaat ctgctggagc ccgtgtgtat
     1321 ttacagacaa tacatgagtc agaaaggaga tcggattcgc ctggaaaata tgcgacttcc
     1381 tcctgcagcg agagctgcct gcaatcaagc gcgcgaagac gcaacctacc tcgagaattg
     1441 aagaatacgt aagccagact agaacttcac aacaagcgtg gtggattttt ccgcaatgcc
     1501 aatgcgcgcc acggagcccg tgttgaattg aatgaaatca gattccattc catcggagaa
     1561 gataacaccg gattcagaca tgtgactttc cacactgatg ctgtttgtgc cctcaatgac
     1621 tccggccacc atttcagtct tactccattt gcttgcgaaa ggttcacgaa ccatgtagac
     1681 cagcttctga tcctcccatt ccagacgcat tcccgccagg ggggaaccgt ccccttgaaa
     1741 tcgagcaatg cccctggtct ggttgaagag cgaactgatc cagcccgtgc tgcccgcagg
     1801 agtggccacg agaatgccgc ttgacatttg tctttctgag cgttcaccat catgaatcgt
     1861 ataacgagct gttatgtgcg acctgggccc gataaaaaga tcgttgaatg cgtacaactt
     1921 ctgcccgtcg ttcatatcta cgcgggccat ggtgaggttt ctcgcctgaa actttccatt
     1981 catgacggcc tgcactgccg cctccgtctg atccggcaaa aagggtaaaa gaactccatc
     2041 aaagcgatcg gggtctggat tgatggcgat cacgggttgg ccttcaaggt atttcgccgt
     2101 gttgaccacc agcccatcct gtcccagaca aatgatcaaa tcctttggag aaaaaagata
     2161 gttgggaagg aagtttcgat ccacgatttg gatttttaga tcccgcggaa ttgccttgag
     2221 tacatgatct cgcgaatgct catagatatc ttcttcgagt tcgtagtcct ggaaggattg
     2281 gccgcgtttt tctacgtaaa acttggcctg cgcctttgtg ttgtatctct tgactgattc
     2341 ctgcagcctc gtgggccgcg tgactacaac ggctttttca acagtggtca ttcgtctttg
     2401 agcaagttct gcagtaaatc cggcgtgatg ttcaatgtgc caatcttctg agcatttttg
     2461 gcaagatcgc gcatggcgcg actgatcagt ttgcgcgatt gcatttgatt ggccatcaat
     2521 acctcaagaa catctggcgg cagcttcatc actgcttcga gttctagtcg cattgattcg
     2581 gcacgagctt tcgcgtattg aatgttgttt tgcacttcca cttccacgag cttgcgcttc
     2641 ttttcctcga gtgcagtgcg cgatagcatc ttctggtctt caatctccgc tttttttctt
     2701 tccacggatt ccacggtctt catcttttct tcttctacgg cgcgctgttt ttcctgtacc
     2761 gcaatctcag cgttcatctg ttcttctcga atcttgcgat tcttttcttc gatagcaatc
     2821 tgagtttgaa gttcgttttc cttaatccgg cgttcttgct ccaccgctga gttgcgacgt
     2881 tcataaatcg catcgtctgc ctctttgtag agcttttcgc gcgcggaggc ttccagggcc
     2941 ctggacatat caggagtggg cgcgatcttt agtatagaaa gatcaagtat ttcaattcca
     3001 agggctgaca gaagttcact ggacttgagc ttacccgcag cgtgttgaac gagcgcggac
     3061 gcacctgcaa gggcatcgcg cagactcatc tctttaaatt tttcgcgaat ggccagctga
     3121 acggaattcg taacgcgcac gccaagcttc tctgctccgt cgcctgtata attcgaaaac
     3181 acatccacgg aaaagtcaag aagtgctgcg agcttttctg gatcgcgtac gcgataggta
     3241 gcctgaccct ggattgtgat ttcctggaag tcgtttgaga cttcattgaa aatgaacgga
     3301 atatcacggc tctctgccgg aagaatcaca acactggaag aaggcgcgta gtagaagaag
     3361 gcaagaccag ctccgtgttt tcgcagctta ccaccgcgaa agagcataac gtattctgtg
     3421 gggcgagtct tgatgtagcg aattccaagc attcggctct tatagcttca tgtggaaaaa
     3481 aagtaaaccc gttttggaat ctgggcagag gaggattcga acctccgaag gcgaatgccg
     3541 ccagatttac agtctggtcc ctttagccac tcgggaatct gcccatatgc tgcctgtagg
     3601 cttcgaaccc acgacctact gatta
//
LOCUS       DAORFF010000415         3613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_16050_length_3613_cov_4.102867, whole genome shotgun sequence.
ACCESSION   DAORFF010000415 DAORFF010000000
VERSION     DAORFF010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16050_length_3613_cov_4.102867"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PKK76_14685"
     CDS             complement(<1..907)
                     /locus_tag="PKK76_14685"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNL69777.1"
                     /translation="MKSLILCAALWFAANLYASPLEEIIDQHPLVQSQSATAGASRHA
                     ARSTMFGYPDPEIEISRSRGREDMIQALPDQMVDRKKITGSEFEVRQPIPFPGKGTVE
                     SLRLGAKADEETLRIRLIKNRMAREFLMLRTRERLIQARIKLNSQTQERLRTLETLAR
                     IRYETGKTPLVDLSMVHVRESEAALAVNQAQSEYASVHRAVEYYTTSSGETHNMSDDQ
                     VDSELAELETRVRNLAAKFEEHSLEMKMAESQIKQGDLDDTRAWMNYLPDFEVFAGYG
                     KENKSSAGLRDWTSEKFYKAGVTIRV"
     gene            complement(943..1266)
                     /locus_tag="PKK76_14690"
     CDS             complement(943..1266)
                     /locus_tag="PKK76_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69778.1"
                     /translation="MSSLRRAVAIPLLPAVVLKAGLCMFLFAAKPAADVPPCHKSQKQ
                     EQCLNCCKVIPGVEVDAFSMPAIVPLASVRLEAPRWLSVQVATKALVHEDRGKTYLRT
                     RSLLI"
     gene            1359..2252
                     /locus_tag="PKK76_14695"
     CDS             1359..2252
                     /locus_tag="PKK76_14695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNL69779.1"
                     /translation="MFFNVGAGHHHGPPTVTRAFVLGVIINSAFVVMEAAAGLFLGSI
                     ALLTDAGHNLADVGSLLVGLVAARLAEKRSSSVYTYGLGKSTVLAALINATFLLVSTG
                     AIGYAAIVRLTSPVPIAGEKVALVAFVGIIINGASALLFFKDRHHDLNVRAAYLHLLA
                     DAGISLGVVVAGFVVYLTGWMWFDPAVGILVGVAILYGAFSLMRDALRLSLDGVPPAL
                     ELSTVRDYLLSVDRVCDVHDLHVWAMSTTETALTAHLVTDDTASLSFEEINEELFHRF
                     HISHATLQVEPVGGADCRQRC"
     gene            complement(2256..2522)
                     /locus_tag="PKK76_14700"
     CDS             complement(2256..2522)
                     /locus_tag="PKK76_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69780.1"
                     /translation="MKNSILISAALAGILAGGTCKEAPKAQVSDEGKCYGVNSCKGTG
                     SCASKEHGCAGLNTCKGKAWLKMKSKECADKSGKFEPVEDGKYM"
     gene            2608..3171
                     /locus_tag="PKK76_14705"
     CDS             2608..3171
                     /locus_tag="PKK76_14705"
                     /inference="COORDINATES: protein motif:HMM:NF016189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HNL69781.1"
                     /translation="MESSTFWFGIVPVIAFVLLESFAGKRMAIVIALVLACAELVFSL
                     VVYGAIDELTILAFVIVFIAGAISVKTGNDLYFKLQPALVSVATAAAFFIYYYLLDRP
                     LLTFMMHKYLPSMLAQLPADRQTLFEGTMRVLSRDLIFWFLLHAAATAYAAMRLSKWW
                     WFAIRVPGLYLMLFLATFIAVRGVIPA"
     gene            3255..>3613
                     /locus_tag="PKK76_14710"
     CDS             3255..>3613
                     /locus_tag="PKK76_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69782.1"
                     /translation="MKQDIQTTDSALPERLRPPQTLNLVLSVASVFAWIGLMYAASRA
                     ESMWVVALIVLPFSFCILTIYSLIHECEHGLFHTDRRINEVAGILLSVFFPGSFSFLR
                     YCHVGHHSRNRSECELF"
BASE COUNT          758 a          906 c          918 g         1031 t
ORIGIN      
        1 gaacgcgaat cgtaacccct gccttgtaaa acttttcact ggtccaatcg cgtaaaccgg
       61 cggagctctt gttttccttt ccataaccgg cgaacacttc aaaatcagga agatagttca
      121 tccacgcacg cgtgtcgtcc agatctcctt gtttgatctg cgactcggcc attttcattt
      181 ccagagaatg ttcctcaaac tttgcagcca gatttcgcac gcgtgtttcc aattcagcga
      241 gctcggaatc tacctgatca tcggacatgt tgtgtgtttc cccggaggac gttgtatagt
      301 attcgaccgc gcgatgaaca gacgcatatt cactctgtgc ctggttcacc gcgagagcag
      361 cttctgattc tcgaacatga accatggata gatccaccag aggtgtcttt cctgtttcgt
      421 atcgaatgcg cgcaagggtt tcaagagtgc gaagcctttc ctgcgtctgg gaattcagtt
      481 tgattcgcgc ctgaatgagc ctttcgcgcg tgcggagcat caggaattcc cgcgccatcc
      541 gattcttgat caaccggatt cgaagtgttt cttcgtcagc cttcgcaccg aggcgaaggg
      601 attctactgt gcccttacca ggaaaaggaa ttggttgacg cacttcaaat tcagatccag
      661 tgatcttctt tcgatccacc atctgatcag gaagggcctg aatcatatcc tctctgccgc
      721 gactgcgcga gatttcaatt tctgggtccg gatacccaaa catcgtgcta cgggccgcat
      781 gtctcgacgc tcctgcagtc gcagactggc tctgcaccag cggatgttga tctatgattt
      841 cctcaagcgg tgaagcatag aggttcgcag cgaaccataa cgctgcgcaa agtattagcg
      901 atttcatgtt tcctccttcg cgttttgcag gcgaaggaga ttctagatca ggagggaacg
      961 cgttcgaaga tatgtttttc cacgatcttc atgaaccaga gcttttgtgg caacctggac
     1021 ggacagccag cgcggagctt ccagccgaac ggacgccagc ggaacgatag ctggcattga
     1081 aaatgcgtcc acttccacgc caggaatcac tttgcagcaa ttgaggcatt gttcctgctt
     1141 ctggcttttg tggcacgggg gcacatctgc cgcgggtttg gccgcgaaca aaaacatgca
     1201 gagccctgct tttagcacga ccgctggaag aagtggaatg gccacagctc ttcgcaagga
     1261 actcatacct tacctaatga gtcggatcaa acgatcgtca actaaaaaag ggagagcgcg
     1321 attgtgcaaa aaagcgtgac gcagcgcgaa gcaatggaat gtttttcaat gtgggcgccg
     1381 ggcatcatca tggacctcca acagtcacgc gagcgtttgt gctgggcgta atcatcaaca
     1441 gtgcattcgt tgtaatggaa gccgcggccg gattgtttct aggatccata gcccttctga
     1501 cagacgcagg acataatctt gcggacgtag gtagccttct cgtagggctc gtagccgctc
     1561 gcctggcaga gaagagatcc agttctgtat acacctacgg tctgggcaaa agcacagtac
     1621 tggccgcttt gatcaatgct actttcctgc tcgtttccac gggagccatt ggctatgcgg
     1681 cgattgttcg cctaacgtca ccggtaccga tcgctggaga gaaggtggct ctcgttgcgt
     1741 ttgttggaat catcatcaac ggagcgagcg ctctcttgtt tttcaaagat cgacatcacg
     1801 atctgaacgt gcgtgccgcg tacttgcatt tgcttgcgga tgctggaatt tcactgggag
     1861 tcgttgttgc tggcttcgtg gtctatctaa ccgggtggat gtggtttgat cctgcagtcg
     1921 gtattctggt tggggttgcc attctttatg gagctttttc tttgatgcga gatgcgcttc
     1981 gtctgtctct ggacggtgtc ccgcctgcac ttgagctttc aacggtgcgc gattatcttc
     2041 tttctgtaga tcgagtttgt gatgttcacg atcttcacgt ctgggctatg agtactacgg
     2101 agactgctct gacagcacat ctggtaacag acgatacggc gtcgctttcc tttgaagaaa
     2161 tcaatgaaga gctatttcac cgatttcata tttcgcacgc aacgctgcag gtagagccag
     2221 ttggcggggc ggactgccga cagcgttgct aaatattaca tatattttcc gtcttccact
     2281 ggctcgaact ttccactttt gtctgcgcat tctttgcttt tcatcttaag ccatgctttg
     2341 cctttgcaag tgttcaaccc ggcacaaccg tgttctttgc ttgcgcagga accagttcct
     2401 ttgcaggagt tcactccata gcatttgcct tcgtcactga cctgtgcttt cggtgcctct
     2461 ttacaggtgc caccggcaag gatgcccgcc agcgcggccg aaatcaaaat cgaattcttc
     2521 atgcattcaa ttcagtgaat gcgcctcttc cgtaaagtga aatacacccg ggattgacag
     2581 cacgcgcggg gcttttcacc tctggttatg gagtcctcta cattttggtt cggcatcgtt
     2641 cctgtgattg cgtttgtgtt actggaatcc ttcgccggaa agaggatggc gattgtgatc
     2701 gcactggtcc tggcctgcgc ggaactcgtg ttctctctgg ttgtatacgg cgcgattgat
     2761 gaacttacga ttctggcgtt tgttattgtt ttcattgccg gtgcgatttc agtcaagacc
     2821 gggaatgatc tatattttaa attgcaaccg gcattggtga gtgtcgcgac cgccgctgct
     2881 ttctttatct attactatct tcttgatcga ccattgttga ccttcatgat gcacaaatac
     2941 ttgccatcga tgcttgcgca attgccggcg gatcggcaga cattgtttga aggaacgatg
     3001 cgagtcttgt ctcgcgacct tatcttctgg ttcctgttgc atgcagcggc cacagcgtat
     3061 gctgcgatga ggttgtctaa atggtggtgg tttgcgattc gcgtgcccgg cctctacttg
     3121 atgctgttcc tggccacatt cattgcagtt cgtggagtga tcccggcctg aggtgattct
     3181 ctgttcgatt ccctcacgcg taaaaaaaat ctgctggaag aaatccctgg gccagtctcc
     3241 catgaaacag aagcatgaag caagacatcc agacaacaga tagtgcactg cctgaacgtt
     3301 taaggccgcc tcaaacatta aaccttgttt tatccgttgc gtctgttttt gcctggattg
     3361 ggttgatgta tgccgccagt cgggcagagt ctatgtgggt ggttgcgctg attgttttgc
     3421 ctttttcttt ttgcattcta actatctatt cgctcatcca tgaatgcgag catggcctgt
     3481 ttcatacgga tcgtcgtatc aatgaagtcg cgggtatttt gctttctgtc ttctttccag
     3541 gatcattttc atttctacgc tattgccatg tgggccatca cagcaggaat agaagtgagt
     3601 gtgaactttt tga
//
LOCUS       DAORFF010000416         3610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_16083_length_3610_cov_3.351899, whole genome shotgun sequence.
ACCESSION   DAORFF010000416 DAORFF010000000
VERSION     DAORFF010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16083_length_3610_cov_3.351899"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PKK76_14715"
     CDS             complement(<1..467)
                     /locus_tag="PKK76_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69783.1"
                     /translation="MANGKRPFVVFFLVAASACIVALFFYAGITHPPTADSDDILRKV
                     PPGTTHIRVGVPTWGGFAGGQYFNEGFNPSTESRYYKNFRILVSFVLKDDIPASREVW
                     KRGDIDVISATADSLAPEIETFLSAEPRVIFQSDWSRGGDAIIADRSIRKPED"
     gene            complement(478..2184)
                     /locus_tag="PKK76_14720"
     CDS             complement(478..2184)
                     /locus_tag="PKK76_14720"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNL69784.1"
                     /translation="MNNPLIVQSDRSMLLEVDHELFDACRAAISRFAELEKSPEYLHT
                     YRVTPLSLWNAASSGMNAEEIVAALEQYSRYPVPKNVIAEVREQISRYGKVKLIKEDD
                     GTLLIISNERAFLNEIAMHRNVQPFIEGRRGDSIIVKKQYRGHIKQALIKIGYPVEDL
                     AGYDEGAKYGFNFRGTTLSGKTFGMRDYQRKSVEVFHASGSREGGSGVIVLPCGAGKT
                     IVGIGVMQIVGAQTLILVTNTLSIRQWKNEILDKTDIPEEDIGEYSGEKKEIKPITIA
                     TYNIITHRKKKDGDFTHFDIFSANNWGLIVYDEVHLLPAPVFRMTSELQAKRRLGLTA
                     TLVREDGLEEDVFSLIGPKKYDVPWKDLERSAFIATAKCIEIRVDLDEELRTRYGIAD
                     DREKYRLASENPDKIKVIERILEEHGRINVLIIGQYLDQLDKLAAHFNFPLITGKTPL
                     PEREFLYAGFRTGKVPCLIVSRVANFSIDLPDASVAIQVSGTFGSRQEEAQRLGRVLR
                     PKPDDNMAYFYSLVSRDTTEERFGQNRQLFLTEQGYEYQIFTFEQYRELAGTAKATIL
                     SR"
     gene            2315..>3610
                     /locus_tag="PKK76_14725"
     CDS             2315..>3610
                     /locus_tag="PKK76_14725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNL69785.1"
                     /translation="MNTRFFPGLLIVLSLAFLVPGTGGKDFPGQGDEVMHLVTVQQSL
                     ASKSYIVPVVDGVPNYYKPPLLFWAGMVTEAVLGPGLFGPRFSSILFTTGIVLLMYYL
                     MVVWGVRPWVAVVASIAYLCSIGTMKFGRLLMMEQGMAFFYLLFVFLLLEHYRTGRIR
                     PLIVSGLISGIGFLFKGPVFLIYCAFTLIIWASMRIVRFKTDPVRITGLRKFWPTVRT
                     GLIFSAFALVVPVIWAGSVLLFAGVAGKAMLAYFFIVENAGKFAQQDQSEIRILVGWI
                     IYTFPWTVVLFAGLYFALRSRYAYGKVQIAGRLLLMTVVVTTIFHFIPNRKAAYYGIP
                     FAPLLFAGLPMMLRDNSVLERWSRLSLIVLYIVMPALAVLTWLGSDSPMSLLLLIPVV
                     AGIFIDIRAKRDSNFLNGSFARDRRLKTLSVAGVLVILVL"
BASE COUNT          740 a          882 c          926 g         1062 t
ORIGIN      
        1 aaatcctcgg gtttgcgaat cgagcggtcc gcgatgatcg cgtcgccgcc acggctccag
       61 tctgactgaa agattactcg aggctccgcc gatagaaagg tttcaatttc aggcgccagg
      121 gaatctgcag tggcagagat tacgtcgata tctccgcgtt tccacacttc acgcgaagcc
      181 ggaatgtcgt ccttgagcac gaaagaaaca agaatcctaa agtttttata gtaacgcgat
      241 tcagtggaag ggttaaagcc ttcattaaag tactgccctc cggcaaagcc accccatgta
      301 ggaactccta cgcgaatgtg agtcgttccg ggcggaacct ttcgcaggat atcgtccgag
      361 tctgcagtcg gtggatgagt aatgccagcg tagaagaaca gagcaacaat gcatgcgctt
      421 gctgccacca ggaaaaagac tacaaagggc ctttttccat tcgccatact tccgcctcta
      481 tctactcaga atcgtagctt tcgcggtgcc cgcgagttcc ctgtattgtt cgaaggtgaa
      541 gatctggtat tcgtagcctt gctctgtcag gaagagctgt ctattctgac cgaatctttc
      601 ttccgtggtg tcgcggctta ccagtgagta gaagtatgcc atgttgtcgt ctggctttgg
      661 gcgaagaacg cggccgagtc tctgcgcttc ttcctgtctg gaaccaaacg ttccactgac
      721 ctggatggca acggacgcgt ctggaaggtc aatggaaaag ttcgcaacgc gggatacaat
      781 caagcaagga actttccccg ttctgaatcc tgcgtagaga aattctcttt caggcagcgg
      841 agtttttcct gtaatgagag ggaagttgaa gtgcgccgcg agcttgtcga gctggtccag
      901 gtactgacca atgatcaaca cgttgatgcg cccgtgttct tctagaattc tttcaatcac
      961 tttgatcttg tccgggtttt cgcttgcgag acgatacttt tcgcggtcgt ccgcaatccc
     1021 gtagcgagtg cgaagctctt cgtctagatc cacgcgaatt tcaatgcatt ttgccgtggc
     1081 gatgaaagcg cttcgctcga ggtccttcca gggtacatcg tacttttttg gaccaatcag
     1141 agagaatacg tcttcttcca gtccatcttc tctgaccagg gtggcggtga ggccgagcct
     1201 tctttttgcc tgaagttcag acgtcatgcg gaatacaggt gcgggaagga ggtgcacttc
     1261 atcatatacg atgaggcccc agttgttcgc gctaaaaatg tcaaagtgag tgaagtcccc
     1321 gtctttcttc tttctatgag tgatgatgtt gtaagtcgca attgtgattg gcttgatttc
     1381 tttcttttcc cctgagtatt cgccgatgtc ctcttcaggg atatccgttt tgtccaggat
     1441 ttcatttttc cactggcgga tggacaacgt gtttgttaca agaatcagag tttgtgcgcc
     1501 aacaatctgc atcactccga ttcccacaat ggtcttgcct gcgccgcaag gaagaacgat
     1561 cactccagag ccaccttcgc gtgaaccgga tgcatgaaac acttctactg atttgcgctg
     1621 gtagtcgcgc attccgaaag tcttcccgga aagagttgta ccgcggaaat tgaatccgta
     1681 ctttgctcct tcgtcatagc ctgccaggtc ttccacaggg tagccgatct taatgagtgc
     1741 ttgcttgatg tgaccgcggt attgcttttt gacgatgatg ctgtctccgc gtcttccttc
     1801 gatgaagggt tgcacgttgc gatgcatggc aatttcattg aggaacgctc tctcattgga
     1861 gataatgagt agagtcccat cgtcttcttt gatgagtttt actttgccgt aacgtgagat
     1921 ttgttcgcgc acttctgcga tcacattttt gggaaccggg tagcgtgagt attgttccag
     1981 ggcggccaca atttcttccg cattcattcc gctcgacgct gcattccaca gggagagagg
     2041 agtcacacga tacgtatgca gatattcggg gcttttttca agctctgcga acctgctgat
     2101 ggctgcgcgg cacgcgtcaa atagctcgtg gtctacttcg agcagcatgc tgcgatctga
     2161 ttgaacgatt agaggattgt tgatatccgg catcgtggcc atacccccga aaaaaatgcg
     2221 tgcggtcaag ccttttaagc cgcctgtttt ctacgcatag ttaaagaaaa aaagtgacca
     2281 gggttcgccc cgttgttttt ctgatccagc actcatgaac actcgatttt tccctggtct
     2341 actgatcgtg ctctctcttg ccttcctggt tccgggcacc ggcggtaagg attttcccgg
     2401 tcagggcgat gaggtgatgc atctggttac agtccagcag agtctcgctt cgaaatctta
     2461 catcgtgcct gttgtggacg gcgttcccaa ttattataag ccaccgcttc tcttctgggc
     2521 gggaatggtc accgaagctg tcctgggccc ggggcttttt ggtccgcgat tctcctccat
     2581 tctttttaca accggaattg ttcttcttat gtattactta atggttgtct ggggcgttcg
     2641 tccctgggta gccgtggtcg catctattgc ttatctctgc tcgatcggca ccatgaagtt
     2701 tggtcgcctt ctcatgatgg agcagggaat ggccttcttc tatcttctgt ttgtgtttct
     2761 gttgcttgag cattatcgca caggcagaat cagacccctg attgtcagcg gcttgatttc
     2821 agggattggc tttctattca aaggccctgt ctttttgatc tattgcgctt ttacactgat
     2881 catctgggcc agcatgcgaa tcgtcagatt caaaacggat cctgtgcgaa tcactggttt
     2941 gcgaaaattc tggccaaccg ttaggactgg tttgatcttt tcggccttcg cgctcgttgt
     3001 tccggtgatc tgggcggggt ccgtactcct ttttgctgga gttgcaggca aggcaatgct
     3061 tgcgtacttc tttatcgttg agaatgcggg gaaatttgcg cagcaggatc aatctgaaat
     3121 tcgaattctg gtgggttgga ttatctacac tttcccgtgg actgttgtac tttttgcggg
     3181 attgtatttc gctcttcgca gcagatatgc gtacgggaag gttcagattg ccggtcgcct
     3241 tctgctcatg acagttgtag tgacaacaat ctttcatttc attcccaatc gcaaggcggc
     3301 gtattacgga attccgtttg ctcctctttt gtttgccgga ttgccaatga tgcttcgcga
     3361 caattcggtc ctcgagagat ggagtcgctt gagtttgatt gttctctaca ttgtgatgcc
     3421 agcacttgca gttctgacgt ggctgggatc agattcgcca atgtctttgc tgttgctcat
     3481 tccggtggtc gcgggtatct tcattgatat tcgcgcaaaa cgagattcca atttcctgaa
     3541 cggctctttt gccagggata gaagactcaa aactctgagt gtggccggag tcctggtcat
     3601 tctggttctt
//
LOCUS       DAORFF010000417         3600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_16170_length_3600_cov_3.353738, whole genome shotgun sequence.
ACCESSION   DAORFF010000417 DAORFF010000000
VERSION     DAORFF010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16170_length_3600_cov_3.353738"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PKK76_14730"
     CDS             <1..666
                     /locus_tag="PKK76_14730"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HNL69786.1"
                     /translation="STDLRTSPNTCTSSTAATAAMSTEARRNPNNALICAFSTVAANA
                     TMFIRVAVVVAFVDFSLLTRLVLPLGAMALVAILAAVILWMIAARRQSGVDVVDRRQL
                     ELSNPFSIGPALKFAVFFVFVLFAAKFAQTMFGNSGLYAAAAVSGLADVDAITLSIAE
                     QAGNGALERITGAMGIIIAVVSNGIVKTGIAFYSGDLRFGRLIGVCLGAATGTGLLLA
                     SFS"
     gene            complement(690..1544)
                     /locus_tag="PKK76_14735"
     CDS             complement(690..1544)
                     /locus_tag="PKK76_14735"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNL69787.1"
                     /translation="MRSLSARPLLICALFLNIALLATPEQDHSLRIMFWNVHNLFDTA
                     NDPRTRDDDFTPSGKEKWTDEKLRIKIKALASVIREVNPDVGGFAEVENIEVLRMLGT
                     EAGYPYAYLIERGDQRGIDTGVLSKTSLQELQNKGPGRGLIHFKIKGVTIAFAHWKSK
                     RGGKNQTEEKRLQAARYAVTMTDRLLLTGDFNEGPHEKAREVLEEAGFRNLVTGPCSS
                     FFSGKKGTSKSCIDGAYLRGECISGRAEIVHPHSLAQGSKPNPEISDHLPVVTILNFC
                     EKESESAR"
     gene            complement(1548..2849)
                     /gene="murA"
                     /locus_tag="PKK76_14740"
     CDS             complement(1548..2849)
                     /gene="murA"
                     /locus_tag="PKK76_14740"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNL69788.1"
                     /translation="MQSPVFRVRGGVPLSGTIRPQGNKNEALPLIAASILTDEPITLS
                     NIPDIEDVRLMVDIVRSLGSSVQEDFQMGPESSIRIHTKEQPQSDLPVDLSTRLRGSV
                     TLAGPLLARTGRVFLPRPGGDRIGRRRLDTHILALEALGAKLKAFPDGFELTTDRLKG
                     ADILLDEASVTGTENAVCAAVLAEGDTVIRHAASEPHVQNLCHFLVSMGAKIEGIGSN
                     ILRIHGVSSLRGTSHRIGPDYLEVGSFISLAAVTRGELLIEDADLENLRMIRLVFSRL
                     GIETRPEGDALRVPGDQSMEIVSDLGGAVPHVDDAPWPGFPSDMTSVALVTATQCKGT
                     LLIHEKMFESRLFFTDRLISMGARIVLCDPHRAVIIGPSPLSGSRISSPDIRAGMAML
                     IAALCAQGESEIQNIIQIDRGFAHIDERLRSVGALIERAEG"
     gene            2896..>3600
                     /locus_tag="PKK76_14745"
     CDS             2896..>3600
                     /locus_tag="PKK76_14745"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNL69789.1"
                     /translation="MSIFRRLLRSLTWHWKEKIICLLMASGLWFYVDNLKIGTISLNV
                     PVSYRNKPVDLDFAQDPPRFIEVKLRGDKERLNFPTTKLRAEVDLKGATAERKTYAYS
                     IDTRLFPENVEMVDTVTSLHIQLEKSMVRFVVLKATTMGSPKSGFKRGRVLIMPDRAK
                     VQGGTERMQSVREIMLPPIDITGASSNVMRSVPVVEPPGTTLLSPPEVEVTVVIVSDE
                     KESEKTLEIPVTFRNAS"
BASE COUNT          822 a          931 c          949 g          898 t
ORIGIN      
        1 agcaccgatc tacgcaccag ccccaacacc tgtacttcct ccacagccgc aaccgcggcc
       61 atgagcacgg aagctcggcg aaatccgaac aacgcgctca tctgtgcctt ttccaccgtc
      121 gcagcaaatg ctaccatgtt tatccgagtg gcagtcgtgg tggctttcgt agatttttct
      181 ttgctgactc gccttgtctt gcctctgggt gcgatggctc tcgtggcgat tctcgcggcc
      241 gtgattctct ggatgattgc cgcacgtcgt cagagcggcg tcgatgttgt tgacagaagg
      301 caattagagc tcagcaaccc attctccatt ggacctgctt tgaaatttgc ggtttttttt
      361 gtattcgttc ttttcgcggc aaaattcgcg cagaccatgt tcggcaactc cggtctgtac
      421 gcggcggcag ccgtgagcgg tctggcggat gtggatgcga tcacactcag tatcgcggag
      481 caggccggga atggagcact tgagcgcata acgggtgcta tggggatcat cattgctgtt
      541 gtttcaaacg gcatcgtgaa gactggcatc gcgttttatt caggagatct caggtttggt
      601 cgcctgatcg gagtctgtct gggcgcggcg actggaacgg gtttgctact cgcctctttt
      661 tcctgaaatt cgcaaagtgt agaattgcgc tagcgcgcac tctcagattc tttttcgcaa
      721 aagttgagga tcgtaactac cgggagatga tctgaaatct ccggattggg cttcgaccct
      781 tgagccagag agtgcgggtg aacaatttct gcacggcctg agatgcactc tccgcgcaga
      841 taagcgccgt ctatgcagct tttgctcgtc ccttttttac ccgaaaaaaa agaggagcaa
      901 ggaccggtaa cgagattgcg aaagccagct tcctcaagaa cctctcgcgc tttttcgtgc
      961 gggccttcgt taaaatcgcc tgtcagaagg agccggtctg tcattgtgac cgcgtatcgc
     1021 gctgcctgca gacgtttctc ttccgtctga ttcttgccac cgcgctttga tttccagtga
     1081 gcaaatgcaa tagtaacccc tttgattttg aaatgaatca aaccacggcc gggccctttg
     1141 ttctgcaact cctgaagcga tgtttttgaa agaaccccag tatcaatccc tctctggtct
     1201 ccgcgctcta tcagatacgc gtagggatag cccgcctcag ttccaagcat tcgtagcact
     1261 tcgatgtttt ctacttccgc aaatccgcct acatccgggt tcacctcgcg gatgacagat
     1321 gcaagagcct tgatcttgat acgaagcttt tcatcggtcc acttttcttt tccggatggg
     1381 gtaaaatcgt catcgcgcgt tcttggatca ttggctgtat caaacaggtt atggacgttc
     1441 cagaacataa tgcgaagaga gtgatcttgt tctggcgttg ccagcaatgc aatgttaaga
     1501 aagagagcgc aaattagaag ggggcgtgcg ctgaggcttc gcactcgtta accctctgcg
     1561 cgttcgatca aagcgcccac actgcgcaac ctttcgtcaa tgtgagcaaa gccacggtct
     1621 atttgaatga tgttctggat ttccgattca ccctgtgcgc agagtgcagc gatgagcatg
     1681 gccataccag ctcgaatatc ggggcttgag atccgtgaac cggacagagg ggatggtcca
     1741 atgatgacag cgcgatgcgg atcgcacagt acgatgcgtg cacccatgga aatcaaacga
     1801 tccgtaaaga agagtctgga ttcgaacatt ttttcgtgaa tcagaagcgt gcctttgcat
     1861 tgagtcgcag ttacaagagc gactgacgtc atatcagaag gaaaaccagg ccacggagca
     1921 tcgtctacat gcggtacggc tccgccgaga tcgcttacga tttccatgga ctgatctcct
     1981 ggaactcgaa gggcgtctcc ctctggtcta gtttcaattc caagtcgcga aaagaccagg
     2041 cggatcatac gaaggttttc caggtctgca tcttcaatga gaagctcacc ccgtgtaaca
     2101 gcggccaggg atatgaaact tccgacttcc agatagtcag gtccaatgcg atgcgaagtt
     2161 ccgcgaagcg atgaaacgcc gtgtatgcga agaatgttgg atccaatgcc ttcaattttt
     2221 gctcccatgg aaaccaggaa gtggcataga ttctgaacat gaggttcaga ggcagcatgg
     2281 cgaataacgg tatctccttc cgcaagaaca gccgcgcaca ctgcgttttc tgttccggtg
     2341 acggacgctt cgtctagaag aatatccgcg cctttgagac gatctgtggt taattcaaat
     2401 ccgtccggga atgcttttag ttttgcgccc agtgcttcca gagcaaggat gtgcgtgtcc
     2461 agtcttcttc tgccgattct gtctccccct ggccttggaa gaaatactct ccccgttctc
     2521 gcgagaagtg gtccggcaag ggttacagaa ccccgcagtc ttgtggaaag gtccacgggc
     2581 aaatcgctct gaggttgttc tttcgtgtga attcgaatag agctttcggg ccccatctga
     2641 aaatcttcct ggacgctgga ccccagcgat cggacgatgt cgaccatgag gcgcacgtct
     2701 tctatgtcgg gtatgttgga tagcgtgatc ggctcgtccg tcaggatgga agctgcgatc
     2761 aatggaagcg cttcgttttt gttcccctgc gggcgaattg tgcccgaaag cggaactcct
     2821 ccacggacac gaaatacagg agattgcacg ggcaagcctt gtttgcatcg aggcctacgt
     2881 caaggggaat cctgaatgtc gatctttcgc cgtctattgc gcagcctgac ctggcactgg
     2941 aaagaaaaga tcatctgtct gttgatggcc tcgggcctct ggttctacgt tgataatttg
     3001 aaaattggaa cgattagtct caacgtgcct gtctcctatc gcaacaagcc cgtggacctg
     3061 gactttgccc aggatcctcc gcgtttcatt gaagtgaagt tgcgcggaga taaagagagg
     3121 ttgaattttc ccacaacaaa actccgcgct gaagtggacc tcaagggcgc taccgccgag
     3181 cgcaaaacat atgcgtattc aattgatacg cgactgttcc ccgagaacgt tgaaatggtc
     3241 gatacggtca cgtccctgca tattcaactt gagaaatcca tggttcgctt cgtagtctta
     3301 aaagccacga ccatgggatc cccgaagagc ggattcaagc gcggacgagt tttgatcatg
     3361 cctgatcgcg cgaaagtgca gggcggtaca gaaagaatgc aatccgttcg agagattatg
     3421 ttgcccccga tagacatcac aggcgcatcc tctaacgtta tgcgatcagt gccagtcgtg
     3481 gagccacccg gaacaaccct gctttcgcct ccggaggtgg aggtcaccgt ggtgattgtg
     3541 tcggatgaaa aagaaagcga aaagactctt gagattccag taacatttcg caatgcatcc
//
LOCUS       DAORFF010000418         3599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_16186_length_3599_cov_2.734199, whole genome shotgun sequence.
ACCESSION   DAORFF010000418 DAORFF010000000
VERSION     DAORFF010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16186_length_3599_cov_2.734199"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PKK76_14750"
     CDS             <1..918
                     /locus_tag="PKK76_14750"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL69790.1"
                     /translation="FDGSMMVSAVFGLVSEESGLVYYVNAEHPFVVLYRRGKASFLDR
                     SMELRKLGIAIIPGILQVRSVPLRPRDVLIIGSDGRDDVAIGEDSLGNRIINEDESHF
                     LRSVEKGDGNLERIEEAIRETGELTDDLSLMRIAFRDDVPLTEESSVDQARDRIAVAR
                     AHARSGRLRQAEEDYRAAAESSPGPAIGREWLKVLHAMGRESERAEIAERYSELHPEV
                     PGFLRVASEALAACGNIEKAVDFGERYRLRQPYNRAGLLHLVDLHMRQGRWTAALRVV
                     EQRLIVDPMDREALELRLLLRQKMGSDDA"
     gene            complement(930..1307)
                     /locus_tag="PKK76_14755"
     CDS             complement(930..1307)
                     /locus_tag="PKK76_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69791.1"
                     /translation="MSVEQVISELRQSGRCIDKPLFSAGDLQTAEQGLGIELPLSYKI
                     LLTTLEPEMTNFYFQPPARHDSFQNLIVFATWDEDQFAFDTERKMRVITLLGTSMRGR
                     EWEDFDGWLAHTWSMSNRPASTE"
     gene            1408..1851
                     /locus_tag="PKK76_14760"
     CDS             1408..1851
                     /locus_tag="PKK76_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69792.1"
                     /translation="MKYSLFLLWILVAATPAFAEECFATPSLYSYTFPKSHLKQYPKQ
                     TVAEMDLSWQDEQTCPHARASISIKLWSGVSYRFFADCAPLGQGRYKCAKGASAASLR
                     FDGKFAWVDWITPVQFDGESGKGNVVLKPKTDDDNFRLDPLKMGP"
     gene            complement(1848..2162)
                     /locus_tag="PKK76_14765"
     CDS             complement(1848..2162)
                     /locus_tag="PKK76_14765"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HNL69793.1"
                     /translation="MTRVTFQNQKGSLRLVADGHSKKTTALDLCSAISVLTETLEASI
                     RLIAKQHSNLEKADGYFDLETEDSPAAQILFAATVLGLRSLEKQFPDALAVRVQGSMA
                     GS"
     gene            complement(2159..2491)
                     /gene="rplU"
                     /locus_tag="PKK76_14770"
     CDS             complement(2159..2491)
                     /gene="rplU"
                     /locus_tag="PKK76_14770"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNL69794.1"
                     /translation="MSPRGFTMYAIIQIAGHQHKVKENDVILTDLTGHDISKEFKCED
                     VIAVGEGESLKVGAPFVKGASVSLKVLEDTRSPKISGMHYKKRKGIRRHWGHRQDLQK
                     LQVLKIQG"
     gene            3048..>3599
                     /locus_tag="PKK76_14775"
     CDS             3048..>3599
                     /locus_tag="PKK76_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNL69795.1"
                     /translation="MSLRTVPVERVPDQLTTEYHERLIKKTRGTGTSVEGARLGMLLL
                     WLADDMQDSVNADLEEFGITENKLGMLIILYLAASEFNRSVTPSELADLVGVERASVT
                     GILDWLEQRKFIVRKPESSDRRKIHVTISNSGKARFHRILKGYWEACDRISRHLTASE
                     QATFEKLTTKLLKGIKVDQKKKKK"
BASE COUNT          831 a          854 c         1012 g          902 t
ORIGIN      
        1 tttgatggca gtatgatggt atcggctgtg ttcggtctgg tgtcggagga aagcggcctt
       61 gtgtactacg tcaacgcgga gcatccgttt gttgtgttgt atcgtcgcgg gaaggcgagc
      121 tttctcgatc gctctatgga gttgcgaaag ctcggcattg cgatcattcc tgggattttg
      181 caggtaagat ccgttcccct gcgtccgcgc gatgtgttga tcatcggctc tgacggacgc
      241 gatgatgttg ccatcggaga ggactcactt ggcaatcgaa tcatcaatga ggatgaatcg
      301 cactttttga gaagcgtaga gaaaggagat ggcaatctgg aacggataga agaggccatt
      361 cgggaaaccg gagaactgac ggatgatctt tccctaatgc gaattgcatt tcgagatgat
      421 gttcctttga ctgaggaatc gtctgtagat caggcccgtg atcgtattgc ggtggcccgg
      481 gctcatgcga gatcaggaag gctcaggcag gcggaggagg actacagagc tgcagccgaa
      541 agttcgcccg ggcctgccat cggacgcgaa tggctcaagg ttcttcatgc gatgggccgc
      601 gagtcagaaa gagcggagat tgcggaacgt tattcggaat tgcacccgga agttcctgga
      661 tttttgcgcg ttgcctctga agccctggcc gcctgtggca atatcgaaaa agcggtggat
      721 tttggagaaa ggtacaggct tcgtcagcca tacaatcgag cgggtctact tcatctggtc
      781 gatttgcaca tgaggcaggg caggtggacg gcagcgctac gagttgtgga gcagaggctc
      841 attgtagacc ccatggacag agaagccctc gagttgcgtc ttctgctccg tcaaaaaatg
      901 ggaagtgacg acgcgtaaat cgagagctgt tattctgtac tggcagggcg gttactcatg
      961 gaccaggtat gtgcaagcca gccatcaaaa tcttcccatt cgcgtccacg catgctggtc
     1021 ccaagaagag tgatcacgcg catttttctt tccgtatcaa aagcaaactg atcttcatcc
     1081 catgttgcga acacgattag attctggaag ctgtcatggc gcgctggagg ttggaagtag
     1141 aagttcgtca tttctggttc gagagtagtc agcaggattt tgtaggaaag gggtagctca
     1201 attccaagtc cctgttctgc ggtttgaaga tcccccgctg aaaaaagagg tttgtctatg
     1261 catctgcccg attgtcgcag ttcggagata acttgttcta cgctcatggg gacatgctgc
     1321 atgagcgcgt tccggggatc aatgatttgt cgacgcaaat cattgaattc tccttgcctg
     1381 ccggcagctc tgtgtttcct attcgttgtg aaatattctc tttttctctt atggattctc
     1441 gtggctgcaa ccccggcctt tgcggaggag tgttttgcga ctccgtctct ttactcttac
     1501 acttttccaa aatcgcatct aaagcagtac ccaaagcaga cggtcgcgga gatggatctt
     1561 tcctggcagg atgaacaaac gtgcccccac gctcgcgcat ccatttctat caaattgtgg
     1621 tcaggcgttt cctatagatt ttttgccgat tgcgcgccgc tcgggcaggg cagatataaa
     1681 tgtgcgaagg gcgcgagcgc agcatcgctt cgctttgatg gtaagtttgc ctgggtagac
     1741 tggatcacgc cggttcaatt cgatggcgaa agcggaaaag gaaacgtagt attaaaacca
     1801 aaaacagacg acgataactt tcgtctggat cctctgaaga tgggccctta gcttccggcc
     1861 atgcttccct gaacgcgcac agctagagca tccgggaatt gcttttccag cgatctgagc
     1921 cccaggacgg tggccgcgaa caatatttga gctgcgggag agtcttcggt ttccagatcg
     1981 aagtagccgt ccgccttttc aagattggaa tgctgcttcg cgatgaggcg aatggatgct
     2041 tctagagttt ctgtgaggac agaaatggcg gagcagaggt ccagggccgt ggttttctta
     2101 gaatggccgt ctgcgacgag gcgcagactt cctttctggt tttgaaatgt gacgcgggtc
     2161 aaccctggat tttgagcact tgaagcttct ggagatcctg gcgatgcccc cagtggcggc
     2221 gaatgccctt gcgcttcttg taatgcatgc cagagatttt gggagagcga gtgtcttcca
     2281 gaactttcag agaaacagaa gcacccttta cgaaaggtgc tcctactttg aggctttccc
     2341 cttcacctac tgcgatgacg tcttcgcatt tgaactcttt ggaaatatca tgccccgtca
     2401 ggtccgtgag gatgacgtca ttttccttaa ctttgtgctg gtgaccggcg atctggatga
     2461 ttgcgtacat agtaaaaccc cgaggagaca tgatttttgg ccccagtcgg cgggtcaagc
     2521 gcgtacctgg gtgtttcctg acccaggtgg ctgccctccc tctggagggc caggtgctct
     2581 ggcggaaact gcctgcctgg atacggtctt gcgggctacc tgagttttga cggaaatgtg
     2641 cttctatggc tcggcgccga tggctccgcc ccgatggctc cgcccaattt gccgacggtg
     2701 ccggaatccc tgcggtacat ggttcttgaa aggtcgccta acgttatttt ttggagagca
     2761 ggtctgcaat tctggttcga ttgcgctttc gcgctacgcc aggagcgtcg agtccgtctt
     2821 tgtcccgcaa atctgttctt gcaccaagtg atagcagcaa tttgaccgct tctatttgat
     2881 cgcatctgac cgcggatgat tccggatgca actgcagcac aactgcatat actcaaaaag
     2941 agcacagagc tggccaggcg aattgagttc atgaagcata cgagatcgag ataaaccctt
     3001 gtgcaatcaa gaaatgtcat ccgcatttcc tgctttcctg attcaatatg agtttgcgaa
     3061 ctgttccagt ggaacgagtt ccagatcaac tgacgaccga ataccatgaa aggttgatta
     3121 agaaaacacg cgggacgggg acttcggtgg aaggtgcgcg tctgggcatg ttgcttctgt
     3181 ggcttgccga cgacatgcaa gattctgtca atgcggacct ggaggagttt ggaatcacgg
     3241 aaaacaagct tgggatgttg atcatactct acctggcagc cagcgagttt aatcggagcg
     3301 tcactccgtc cgaacttgca gatcttgtgg gcgtggagcg agcatctgtg accgggattc
     3361 tagactggct cgaacaaaga aaattcatcg tccgaaagcc agagagcagt gatcgcagga
     3421 aaatacacgt aaccataagc aattccggca aagcccggtt tcacagaatt ttgaaaggct
     3481 actgggaagc atgcgatcgc atttcgcgac atctaaccgc gtctgagcaa gcaacgttcg
     3541 agaaattgac cactaaactg ctcaaaggca ttaaagtgga tcagaaaaag aagaaaaaa
//
LOCUS       DAORFF010000419         3595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_16211_length_3595_cov_3.651977, whole genome shotgun sequence.
ACCESSION   DAORFF010000419 DAORFF010000000
VERSION     DAORFF010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16211_length_3595_cov_3.651977"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1931)
                     /locus_tag="PKK76_14780"
     CDS             complement(30..1931)
                     /locus_tag="PKK76_14780"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL69796.1"
                     /translation="MVFSRMPRKNYNKTLNMRRTVFFVFLIAFPLLSGCTFDSTESLS
                     GDWNAALNFLPGSRDPFAKLEWKAIGTPAKLSRESGIDTFRYRGWIILQRPLPLDLGQ
                     KALEGKLALHIGRVSDAAIIYLDDTIAAKIGNESPYQSGFRHFTGSLPWKGTSPPKVL
                     RIAMLATEQFPATISGLEIQGGSTEDVFAHANYLELISCFFVAIYLSIGCYHMILGLR
                     RLEDLYNIHFGLFALLLAAFYAFQTYTFETVSGNQVLLRERLEETVLFLLGPPLLFFL
                     SDYFYGTLSRFGLLAQLFFTGMVIAVWFSDYRHLMVLRDVWHLMAFAVMVYVMFYIAR
                     ECYEKKRDAMYMAPGIGLLMLTGIHDIMVARGLLYSIPGTSKFSFLILNAGLAVILAN
                     RVIRLQQRVTDLNAHLEQRVESRTIELAAALSDLQNKDKRIQNEMELAREIQLGILPE
                     MPLLWKEFRITARYQSMRKIGGDFYDVQQTRDGKLAILMADVSGHGIPASLITTMTKI
                     SFTTALQHSSSPAEVLRAINDEILAHITSHQYLTAALIVISENGDALYASGGHCPAVL
                     IGKSDIRLVSSAGSILGAMREAPVAIEEIPVEIKSGDRLLLYTDGITEARHNRENFGM
                     DRLRQICAE"
     gene            1930..3540
                     /locus_tag="PKK76_14785"
     CDS             1930..3540
                     /locus_tag="PKK76_14785"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNL69797.1"
                     /translation="MNNPLSRLPVVGKWFREPVAGRVYHGIDSIRRRASLFHYPLRAF
                     FRAVARTQHSEIQAIGLENIPPDGAVFLVGNHPNSYVDFLNLSVVLRHPVATAAKDTI
                     TKTPLVGGFLRDYALLVPVSRVQDKEETGATEEERAAANEEMVREAVELLVRGRLFNI
                     YAEGRSTDARRLNKIKLGFMMLAIQAEKAYDFKLNLRIVPYGYFYDRINKFQSSVCII
                     FGRPFKLRSLVELPANFLSLSAKEQLVYEKKLMLAGKEKLQSEIEKLIISIHDPKLVY
                     LIDGITAVYVLSPVKYMGPFENVREKYALCKMISETIQIAAKSEEGAVRVETLKLLLK
                     DYQTSLAEARVADALVRREYTWTSLGYHLKGLVMGILYAPLSFYGYLANVPSRQAARF
                     MRYYVIEVKKRPMVDGDEQSILAAAAAGAIAYPIYYIVAYYLFRHFVMPWMNGFLSIS
                     THLKDLAFLPAEHPVFLSILGATGAVYLMARLWRFSLFHGRRFRQGVRFLLDAISEIF
                     LRSKLQKLREKRSELIDAMDFLIGDYYH"
BASE COUNT          889 a          843 c          922 g          941 t
ORIGIN      
        1 gcttatcgct gttgtggatg agttccttat cactccgcgc agatttggcg aagtcgatcc
       61 attccgaaat tttcccgatt gtgcctggcc tctgtaattc cgtccgtata aagaagcaga
      121 cgatcgccgg acttgatttc gacggggatt tcttctatgg ccacgggagc ttcgcgcatc
      181 gcccccagga tggagccggc gctggatact aaacgaatat ccgattttcc aatcaacacc
      241 gccgggcagt ggcctccgct tgcatacagc gcatctccat tttctgaaat cacaatcagt
      301 gctgccgtta gatactgatg gctggtgata tgcgccagga tctcatcgtt gattgcgcga
      361 agaacttctg ccgggctgct tgaatgctga agcgctgttg taaatgagat cttggtcata
      421 gttgtaatga gagaagccgg gattccgtgc ccggaaacgt ccgccatgag gatggccagc
      481 tttccatctc gtgtttgttg cacatcatag aaatctccgc caatcttccg catagactgg
      541 taccttgccg ttatgcggaa ttccttccac agaagaggca tttcagggag aataccgagt
      601 tgaatctctc gtgccagctc catttcattc tggatgcgtt tgtctttgtt ctgcaagtcg
      661 gaaagagcgg ccgcaagttc aatggttcta ctttccactc tttgttcgag atgcgcgttt
      721 aagtcagtga cgcgctgttg tagacgaatg actcgatttg cgaggatcac tgcaagcccg
      781 gcgttcagga tcaggaatga aaacttactg gtccctggaa tggaatagag caagcctcgc
      841 gccaccatga tgtcgtgaat tcccgtaagc atgagcaagc cgattcctgg tgccatgtac
      901 attgcatcgc gcttcttttc gtagcactct ctggctatgt agaacatcac ataaaccatc
      961 acggcaaatg ccatcaagtg ccatacgtcg cgaagaacca tcaggtggcg gtagtccgaa
     1021 aaccatacgg cgatcaccat acccgtgaag aaaagttgtg ccagtaaacc aaatcgcgag
     1081 agagttccgt aaaaatagtc tgatagaaag aagaggagtg gaggccccag gagaaacaga
     1141 accgtttctt ccaggcgttc cctgagcaat acttgatttc cacttactgt ttcaaaagtg
     1201 tatgtttgaa acgcgtagaa tgcagcgagc agaagcgcga aaagtccaaa gtgaatgttg
     1261 tatagatctt cgagccttct cagtcccagg atcatgtgat agcagcctat gctcaaatag
     1321 atggccacaa agaagcagct aatgagttcc agataattcg catgggcgaa tacatcttct
     1381 gtggatcctc cctgaatttc caggcctgag atcgtggccg gaaactgttc ggtggcgagc
     1441 atggcaatgc gcagaacttt tggcggagaa gttcctttcc aggggaggct gccggtgaaa
     1501 tgtctgaacc ctgactggta gggcgattcg tttccgatct ttgctgcgat ggtgtcatct
     1561 agataaatga tggctgcgtc tgaaactctt ccgatgtgca aggcgagttt gccctccagg
     1621 gccttctgcc ccaggtccaa aggcaagggt ctttgcagta tgatccaacc tctgtagcga
     1681 aatgtgtcta tgcccgattc gcgcgaaagc ttggcgggcg ttccgattgc cttccactcc
     1741 agtttcgcaa atggatctct tgacccgggc aaaaaattaa gggctgcgtt ccagtcacct
     1801 gaaagcgatt cagtcgagtc aaacgtgcag cccgaaagca atggaaatgc aataagaaag
     1861 acgaaaaaaa cagttcttcg catgttcaag gttttgttgt agttcttccg gggcatgcga
     1921 gaaaagacca tgaacaatcc tctgagccgg ctaccggtag taggtaagtg gttccgcgaa
     1981 ccggttgcgg gccgagtcta tcatggcatt gattcgatcc gcaggcgtgc aagtctgttt
     2041 cattatccgc ttcgagcttt tttcagagcg gtggctcgca ctcagcacag tgaaatccag
     2101 gcgattggtc ttgagaacat tcctccggat ggagcggtct ttctggtagg caatcatccc
     2161 aattcctatg ttgatttttt gaatttgagt gtggtcctcc gtcatccagt tgccactgcg
     2221 gccaaagata cgattacgaa gacccccctt gtgggtggat ttttgcgaga ctacgcgttg
     2281 ctcgttcctg tttcgcgcgt gcaggataaa gaggagacag gtgctacaga agaagagcgt
     2341 gcggccgcca acgaagagat ggtgcgcgaa gcagtagagc ttctagtgag gggccgtcta
     2401 tttaatattt acgcggaagg aagatccaca gacgccagac gactgaacaa aatcaaactt
     2461 ggattcatga tgctggccat tcaggcagag aaagcatatg acttcaaatt gaatcttcgc
     2521 attgtaccgt acggatattt ttacgatcgc atcaacaagt ttcagagttc tgtttgcatt
     2581 atctttggaa gaccgttcaa gctcagaagt cttgtggagc ttcccgccaa ttttctttcg
     2641 ctctctgcca aagagcagct cgtctacgaa aagaaactga tgcttgcagg caaagaaaag
     2701 ctgcagtctg aaattgaaaa actcatcata tccattcacg atccaaagct cgtttacctg
     2761 atagatggaa tcacagcagt ttatgttttg agtccggtta agtacatggg cccgtttgaa
     2821 aatgtgcgag aaaaatatgc tctgtgcaaa atgatttctg aaaccatcca gatcgcggcg
     2881 aaatcagagg aaggtgcagt gagagtggag actctcaagt tactcttgaa agactaccag
     2941 acttctcttg cggaagccag ggttgcagac gcactcgtaa ggcgagagta cacctggact
     3001 tccctgggct accacctcaa aggtctcgtg atggggattc tctatgcacc tctgtcattc
     3061 tatggctatc tggcaaacgt accttcgcgt caggcggcgc gattcatgcg atactatgtg
     3121 atcgaggtga agaaaagacc aatggtggat ggagatgagc agtccattct ggcggcggcg
     3181 gccgcaggcg ccattgccta tcccatatac tatatagttg cttattattt gtttcgccac
     3241 tttgtgatgc cctggatgaa tgggtttttg tccatctcca ctcatttgaa agatctagca
     3301 tttttgcccg cggagcaccc tgtatttctg tctatcctgg gcgcgaccgg ggctgtgtat
     3361 ttgatggccc gactctggag attttctctg tttcacggaa gaaggtttcg tcagggagtt
     3421 cgctttctcc tcgacgctat ttcggagatc tttcttcgaa gcaagttgca aaaattgaga
     3481 gagaaacgtt cagaactgat tgatgcgatg gactttttga tcggggatta ttatcattga
     3541 atgcgagtta tatgaaaaag atcaacttca gtttcgcaat gccagcactg gttgc
//
LOCUS       DAORFF010000420         3583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_16301_length_3583_cov_3.798469, whole genome shotgun sequence.
ACCESSION   DAORFF010000420 DAORFF010000000
VERSION     DAORFF010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16301_length_3583_cov_3.798469"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..561
                     /locus_tag="PKK76_14790"
     CDS             34..561
                     /locus_tag="PKK76_14790"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNL69798.1"
                     /translation="MPYARTGIIYAVIMIALIAGEIVDVVGDFVREGFTIWLCQDVLL
                     LLLVSVGVTYILYTSAADLRGMHIHLEKARQDVASFRSRNQDAIVAMREAILSQFGAW
                     GFTPAEARIADLLIRGYSSRQIAGMLGRSERTVRNQTLSIYRKSGMTGRSDLSAFFLQ
                     DIMGEEKEFPAVQGR"
     gene            571..1347
                     /locus_tag="PKK76_14795"
     CDS             571..1347
                     /locus_tag="PKK76_14795"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNL69799.1"
                     /translation="MPGFPTTTIERKNGVIGVLTLDALPPNNTFNHELLLGFTKALFD
                     LGEKCDAVLVTSANPKFFSNGLDGKFLLEADLKTREETVTAMIRAYGKMISFGKPWIV
                     EIAGHAMAGGAVISCAADYRFMLQGSGRIGFSELAVGLPLPLNYIHGIHRIVQPSAVR
                     LLIEGAAYKPEEALSIGLIDGVAEDAEKLRALVLKRFDAILRMEKEAFLPTRKIYRGD
                     LLRMIERDEEEDVRMAMQLVRSPAFERALGTIAGKNRGQS"
     gene            1344..2027
                     /locus_tag="PKK76_14800"
     CDS             1344..2027
                     /locus_tag="PKK76_14800"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNL69800.1"
                     /translation="MIFLFLAIYLSAAGFSIYCRYNNRPLYNFAKGIPLVLLFIYYLF
                     HPSGSGLYYWFVIALLSGLIGDLLLLREKTFRYGALFFSLGHLFYVGAFYVMGGWPKI
                     GVLVLIFGYMAIYCAFLYKRLEETGGVGNVWLGLPYLFLVSLLLVFASATAGHVAALG
                     VPAQLEPLPLFVIDASIFVGALLFYVSDLVLVWNRNAGTFKEAQFVILSTYYLAQLFI
                     AMGAIGHLN"
     gene            2164..2766
                     /locus_tag="PKK76_14805"
     CDS             2164..2766
                     /locus_tag="PKK76_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69801.1"
                     /translation="MSILVLAPEVALFDGADASQLGQSDPNIARARSYLTTVIPREIV
                     GAVSSRGFLVHTREDDNGQKSLREALLTESSIKGAPIKVIVPVTEQYVLAYRMAVVQR
                     QQVETERAVIGALSCLSLLALQFWSVSTHEEIMNLDIVMMDVRLSRIAWRRHYSENLS
                     QLKFLDNQYFGFAPFTRDRLSHFPTALKLQTAVVPSSEGK"
     gene            2763..3353
                     /locus_tag="PKK76_14810"
     CDS             2763..3353
                     /locus_tag="PKK76_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HNL69802.1"
                     /translation="MTIRESMGIVRICVVALGVLIGCSVDPIRTEMLFKAKSEVAIIM
                     DYQEENRASPESDIIRKRIASLVQAQLIERGFRVLDQSPDVGENFDWRRKDHRDALFP
                     AYGKQPVLLFVVKVTALKTQVESSLPLTCVRFALLDYRDPDSFVEYTVSADVQSVVFS
                     TGEVLHERHIKRPLHDYTQDSIVDTAGWLTIWSEVR"
BASE COUNT          790 a          845 c          952 g          996 t
ORIGIN      
        1 tttacacgca cggcaacaaa aggaatttga gcgatgcctt atgcccgaac agggatcatc
       61 tatgctgtga tcatgatcgc tttgattgca ggcgaaatcg tagatgtagt gggcgatttc
      121 gtgcgagagg ggtttaccat ctggctctgc caggatgtgc ttttgcttct tctggtttcc
      181 gtaggggtaa cttatatcct gtatacttcc gccgcggatc ttcgcgggat gcacattcat
      241 cttgagaagg ccaggcagga tgtggcgtcg tttcgatcgc gcaaccagga cgcaatcgtt
      301 gccatgagag aagccatcct gagtcagttc ggcgcctggg gttttacgcc tgcagaagct
      361 cgcattgctg atttgttgat tcgtggatac tcaagcaggc agatcgcagg aatgctcggt
      421 cgcagcgaaa gaactgtgcg caatcagacc ctttcgattt atagaaagtc tggcatgaca
      481 ggtaggagtg atctgtctgc cttctttctg caggacatta tgggcgaaga aaaagaattt
      541 cccgcagtcc agggtagatg agtctggccc atgcccggct tcccaaccac gaccatagaa
      601 agaaagaacg gcgtcattgg cgttttgaca ttggatgcgt tgccgccaaa caacacgttc
      661 aatcatgaac tgctccttgg attcacgaag gcgctctttg acctgggcga aaagtgcgat
      721 gcggttctcg taacgtctgc caaccctaag tttttttcaa atggcctgga cggaaagttt
      781 ttgctcgaag cggacttgaa gactcgcgaa gaaacggtga ctgccatgat tcgcgcttac
      841 ggaaagatga tttcattcgg gaagccctgg attgtggaga tcgcagggca tgcgatggca
      901 ggtggcgctg taatttcgtg tgccgctgat tatcgtttta tgctgcaggg atccggacga
      961 attggattct ctgaacttgc ggtgggtcta cctcttccgc ttaactatat tcacggaatc
     1021 catagaatcg ttcaaccatc tgcggtgcga ttgctgatcg aaggtgccgc atacaagcca
     1081 gaggaggctc tgtctattgg tttgatcgat ggtgtggcag aagacgcaga aaagctccgc
     1141 gctcttgtgc tgaagcgatt tgatgccatc ctccgaatgg agaaggaagc gtttcttccc
     1201 acgagaaaaa tatatagagg agacctcctt agaatgatcg agcgcgatga agaggaagat
     1261 gttcgcatgg ccatgcaact ggttcgctct cccgcatttg aacgggctct cggcacaatc
     1321 gcaggaaaga acagaggcca atcatgatct ttctttttct ggccatctat ctttctgcgg
     1381 ctggcttttc catctactgt cgctacaaca accggccgct ctacaacttc gcaaaaggca
     1441 tcccgctcgt tttgcttttc atttactatt tgtttcatcc gtccggttcc ggtctttact
     1501 actggtttgt gattgctttg ttgtccggcc tgatcgggga tctgttgcta ttgcgcgaaa
     1561 agacatttcg ctatggggct ttgtttttct ctctgggcca tttgttctat gtgggcgcct
     1621 tctacgtgat gggtggatgg cccaaaatcg gcgttctggt tttgatcttt ggatacatgg
     1681 ccatttactg tgcttttctt tacaagcgcc tggaggagac gggtggagtg ggaaatgtgt
     1741 ggcttggtct gccatatctg ttcctcgtga gtcttcttct ggtcttcgca agcgccactg
     1801 ccggccatgt agcagcattg ggcgttccag ctcaactgga acctctaccg ttattcgtca
     1861 ttgatgcttc catttttgtc ggagcacttt tgttctacgt atccgatctg gttcttgtct
     1921 ggaatcgaaa cgctggaacc ttcaaggaag cgcagtttgt gattttgtcc acatactatc
     1981 ttgcgcaact cttcattgcc atgggtgcca tcgggcattt gaattgattg tgaagtgaag
     2041 ttacgaactg actttgcatt cggacggatc cgaatgaaac tcatattctc gctggtcgtg
     2101 ctgagcggtc tgttgcgttg tcaatcgcaa ccttcagtcg tgtagccgga gcctggcgat
     2161 accatgtcaa ttttagtgct ggctccggaa gtcgcccttt tcgatggagc agacgcttcc
     2221 caactcggtc aatcggatcc taacattgcc agggcgcgtt cctatctcac tactgtgatt
     2281 ccccgggaaa tcgtaggtgc agtttcgagc cgcggattcc ttgttcatac gcgtgaagat
     2341 gataacggac agaaaagtct tcgcgaagcc cttttgactg aaagttcaat aaaaggcgcg
     2401 ccgatcaaag tgatagttcc tgtgacagag caatacgtcc tggcctatcg catggcggtg
     2461 gtgcaaagac aacaggtgga aacggaacgt gccgtcattg gtgccctctc ttgcctttct
     2521 ttgttggccc tgcaattctg gtctgtttct acgcatgagg aaatcatgaa tctagacatt
     2581 gtaatgatgg acgtccgtct ctccaggatt gcctggcgtc gccattattc agagaacctc
     2641 tcgcaactca aattcctgga caaccagtac tttggttttg cacctttcac aagggatcgg
     2701 ttgagtcatt tcccaacagc actcaagctg cagacggcgg tcgtgccttc atccgaaggc
     2761 aaatgacgat tcgggaaagc atgggaatcg tgagaatctg cgtggttgcg ttgggagttt
     2821 tgatcggatg ttccgtcgat ccgattcgca ctgaaatgct ttttaaagcg aaatccgaag
     2881 tggctataat catggattac caggaggaaa accgggcgag ccccgagtcg gatataattc
     2941 gaaagaggat tgcgtcgctt gtccaggcac aactcatcga gcgcggattt cgcgttctcg
     3001 atcagtcgcc tgacgttggt gagaacttcg actggcggcg caaagaccat cgagacgcgc
     3061 tttttccggc ctatggaaag caaccggtct tgctatttgt tgtcaaagtt acggcattga
     3121 agactcaagt tgagagcagt cttcccctga cttgtgtccg cttcgcgttg ctagactacc
     3181 gtgatccaga ttcctttgtc gagtacaccg tatccgccga tgttcagtca gttgtttttt
     3241 ccacagggga ggttcttcat gagagacaca tcaaaagacc actccacgat tatacgcagg
     3301 attcaatcgt cgatacggcc ggatggctga ctatatggtc ggaggtccga tagccagagg
     3361 cgcgaaggct gaaatttatt gggagtaaga tgtggagttc atatggcatt gagatgtatt
     3421 ttaccattct tttttctttt gttcgggtgc agcctggaaa cggcggcgcc gtacattcta
     3481 aaaagtagcg cacatgagtg ggcgagtcag gcagcggatg gacacggctt tctcattttg
     3541 tcgctttctg aacgctccaa ttgcaagttc gaatatcttt cca
//
LOCUS       DAORFF010000421         3572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_16378_length_3572_cov_3.319875, whole genome shotgun sequence.
ACCESSION   DAORFF010000421 DAORFF010000000
VERSION     DAORFF010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16378_length_3572_cov_3.319875"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..2305
                     /locus_tag="PKK76_14815"
     CDS             41..2305
                     /locus_tag="PKK76_14815"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNL69803.1"
                     /translation="MRQTLFALSLLVFTSLFLYVRASANVLPVVSDHAPEQEFSALRA
                     LVHLRNIARSPRPVGSSENERVRHYLVETLRHEGVDVRILKGSAVRTLIPRVHAAGNV
                     YNVLAVIPGKRKDSFLFLTHYDSVPTGPGAADNGASVVSALETIRALSKEKPEDTLQF
                     LFTDGEEAGLLGAASFVSRAENTSNVVAVFDFEARGISGESFLFQTGKKSSGLLQAFL
                     ENASGVAGSSLGPALYDVLPNDTDFSVFKRRNFVGLDFAFIGDVQAYHTSLDRPENLD
                     LRTLQHQGENMLTLARTREILDSNIETYFSPWPGIVLHYGSFVTRSFLILLLILAVLA
                     SRVIALRRSAAAFLYSIACIAVAALIGFGSWPTLSWLNGSTASLLGQPHNYFAYQLYF
                     ISLGVSAVFLIRFFALRRYSTEEVSVAFLWTAAACSVAAVILAPGAAYIFLLPAIACA
                     VCILLSRRFPGGFFSPAFALIFALSIVLIAWDPISGLFLALTLRAGFLAVILVCFYLL
                     LALHFVDLMLSTRHTLILSTVMFALLAAAVRVLSESPGPVLNSLAYGSEVETGKAFYF
                     SCAEKNDSFTSRFFGQAVRTGVIPAFPVMKQCLSGDLFQLATAPVFSNEPPVLQYSSE
                     RRGKKRWIHAKITSPRHASGLIVSVHTNPEIEKMVLFGRELPMQLEYGEGKKGLQFVK
                     RILRLDEWHTIMFAGLDEKGMELELEVNGNSTVEIRVTDRSRTILSQRPPGMIEAPEF
                     PFSDGVFITRTFKL"
     gene            2432..3277
                     /locus_tag="PKK76_14820"
     CDS             2432..3277
                     /locus_tag="PKK76_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNL69804.1"
                     /translation="MIINHNLSAIDSHRTLKFQHWQVDRNMEKLSSGMRINRAGDDAS
                     GLAVSEKMRAQVSGLRQAERNTEDGMSLIQTSEGYLQEVSDIVQRIRVLAVQSSNGIY
                     SAGDRQMIQVEVSQLVDEVDRIASQAEFNKMSLLKGDFARGSRVSSMWFHIGPNQHQR
                     ERVYIQTMTAQALKLKRTDGTLLTLSTAEFSNDAIGVLDDALMRINKQRADMGAYYNR
                     LEHASKGLMVAYENTQASESRIRDTDMAEESVSFTKNQILVQSGTAMLAQANVRPQSV
                     LQLLR"
     gene            3372..>3572
                     /locus_tag="PKK76_14825"
     CDS             3372..>3572
                     /locus_tag="PKK76_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69805.1"
                     /translation="MNTEIDFLRDMMKRLDLFFLEADDHGKIRAISGGLLRLLGYPVG
                     HEISLQSLFDETTVAELFGRKLS"
BASE COUNT          805 a          906 c          892 g          969 t
ORIGIN      
        1 acattcttga ccctctggaa cgattccgca ttcagtgaga atgcggcaaa ccctgttcgc
       61 tctttctctt cttgtcttta cttctctttt tctctacgtg cgtgcaagcg ccaacgtgct
      121 gcctgttgtt tccgatcatg cacccgagca agagttttcg gctcttcgcg cactcgtgca
      181 tctgcgaaac atcgcccgat ctccaagacc agttggttcc agtgaaaatg agagagtgag
      241 gcactacctg gtcgagactc ttcgccatga gggtgtggat gttcgcattc taaagggttc
      301 tgcagttcgc acactcatcc ctcgcgtgca tgcagctgga aatgtataca acgttctggc
      361 tgtgatcccg ggaaagagaa aggactcgtt cttatttctg actcactatg attcggttcc
      421 aacgggaccc ggagcagcag acaacggggc aagtgttgtg tccgctctcg aaaccattcg
      481 cgctctttcg aaagaaaagc cagaggacac tcttcagttt ctttttacag acggagaaga
      541 agcaggactt ctgggagcgg cctcgtttgt atctcgagcg gagaacacgt cgaatgtggt
      601 agcagtcttt gatttcgaag ccaggggaat ttctggagaa tcgtttctat ttcagacagg
      661 taagaagagc agtggtttgc ttcaggcttt tctggagaat gcatctggag tggcaggcag
      721 ttccctcggg ccggctctct acgacgtact gcccaacgat actgatttca gcgtattcaa
      781 gagacgcaat ttcgtaggac tcgattttgc tttcatcgga gatgttcagg cataccacac
      841 ttccctggat cgtccagaaa atctggatct tcgcactctg cagcaccagg gagaaaacat
      901 gctcactctt gcacgcacgc gagagattct ggattctaac attgaaacat acttcagtcc
      961 gtggcctgga attgtgcttc actatggcag ttttgttacg cgctcgtttt tgattctgct
     1021 tttgatcctg gctgtgcttg cttcgcgtgt cattgcctta aggcgatctg ccgcagcatt
     1081 tctgtattct attgcatgca tcgcggttgc ggctttgatt ggatttggtt catggccaac
     1141 cttgagctgg ctcaacggca gcactgcaag ccttctcggg cagccacata actatttcgc
     1201 gtaccagctc tacttcattt ctctgggagt atctgctgtt ttcttgattc gcttctttgc
     1261 tctcaggcga tacagcacag aggaagtttc tgttgcattt ctatggactg cggcggcctg
     1321 ttctgttgca gcagtcatcc tcgcgcctgg cgcagcatac atcttcctct tgccggcaat
     1381 tgcatgtgct gtgtgcatct tactttccag gcgatttcct ggcgggtttt tttcacctgc
     1441 atttgcgctg attttcgcac tgtcgatcgt attgattgcc tgggatccga tttccggact
     1501 gttcctggct ctcactctgc gcgcgggttt tcttgctgta atactcgttt gtttctactt
     1561 gcttctagcc cttcattttg tggatctgat gttgtccacg cggcatacgt tgattctatc
     1621 aaccgtaatg tttgcccttc ttgccgcggc tgttcgcgtg ctgtctgagt ctcctggtcc
     1681 tgttttgaat tcacttgcat acggaagcga agtagaaacg ggcaaggcgt tttacttctc
     1741 ctgtgctgag aagaacgatt cgtttacttc tcgcttcttt ggccaggcag ttcgcacggg
     1801 tgtgattcct gcgtttcctg taatgaagca atgcctgtcc ggagatctat ttcagcttgc
     1861 cactgctcct gttttttcca atgaaccgcc cgtgctccag tactcgagcg agagaagagg
     1921 gaagaagaga tggattcacg cgaagatcac ttcgcctcgg catgcatccg ggctcattgt
     1981 atccgtacat acgaaccctg aaatagagaa gatggttttg ttcgggcgcg aacttccgat
     2041 gcagcttgaa tacggggagg gaaagaaagg tcttcagttc gtgaaacgca ttcttcgcct
     2101 ggatgaatgg catacaatca tgttcgccgg cctcgacgaa aagggcatgg aacttgagct
     2161 agaagtgaac ggaaattcga ccgtggaaat tcgcgtaaca gatcgctcgc gaaccattct
     2221 ctctcaaaga ccacccggaa tgatagaagc gcccgagttt cctttttcag acggcgtgtt
     2281 tatcacaaga acattcaagc tctaaccaaa aaagtcgtcg ttcgacctaa tttttactca
     2341 agcttttccc gaagctgaat gtggaaacgg aaaaaatgag caaggatgct catccgtgac
     2401 cgagttctag aagtttcacg gaggaaactt tatgattatc aaccacaacc tatctgcgat
     2461 cgactcgcac cgtacgctga agtttcagca ctggcaagta gatcgtaaca tggagaaact
     2521 gtcgagtggc atgcgtatca accgtgccgg cgacgatgcc tcgggtcttg cggtttccga
     2581 aaaaatgcgt gcacaggtct ctggcctgcg tcaggcagaa cggaacactg aagacggtat
     2641 gagcttgatc cagacatcgg aagggtatct gcaagaagtt tcagatatcg ttcagagaat
     2701 ccgggtgctg gctgttcagt cttccaacgg tatctactct gcgggtgacc gtcagatgat
     2761 ccaggtggaa gtttcccaac tagttgacga agtggacaga atcgcttcac aggcagagtt
     2821 caataaaatg tctctcctga aaggcgactt tgcaagaggc tctcgcgtct cttcaatgtg
     2881 gttccacatt ggtccaaatc agcaccagag agaacgcgta tacatccaga cgatgactgc
     2941 gcaggctctc aagctgaagc gtaccgatgg cacgttgctc actctctcaa ccgctgagtt
     3001 ctcgaatgac gcaattggtg tcctcgacga cgcgctgatg agaatcaaca aacagcgcgc
     3061 agacatgggc gcctactaca acaggctgga gcatgcttcc aaaggcctga tggttgctta
     3121 cgaaaataca caggcatctg aatccagaat ccgtgatacg gacatggcag aagaaagcgt
     3181 gtctttcaca aagaaccaga tccttgtaca gtctggaacg gcaatgctcg cgcaagcgaa
     3241 cgttcgtcct cagagtgtac tgcagctgct ccgctaaaaa aaatgaccgg ccctggtttc
     3301 agggccggtt ttccttcctc ctccatttaa tacttgcgct gacaacccga ccccccgagg
     3361 ctactcgcgg cgtgaatact gagatcgatt ttcttcgtga catgatgaaa cgactcgacc
     3421 tcttctttct cgaggccgac gatcatggca aaatccgcgc tatttcaggc gggctattgc
     3481 gtcttctggg ctatccagtc ggacacgaaa tcagtctgca gagtcttttt gatgaaacca
     3541 cagttgcgga gttgttcgga cgcaaacttt ct
//
LOCUS       DAORFF010000422         3571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_16382_length_3571_cov_3.443686, whole genome shotgun sequence.
ACCESSION   DAORFF010000422 DAORFF010000000
VERSION     DAORFF010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16382_length_3571_cov_3.443686"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PKK76_14830"
     CDS             <1..849
                     /locus_tag="PKK76_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69806.1"
                     /translation="GQFHIDFTGPGWIFPLFYFRARKFSLMLRGGEKRDRVYLEADHV
                     QFWVSPFDLLRGRLVLWRMRLIHPVLEYVNRQDSSEKNKLLPAPGRFLIKRGRIRHGR
                     IKVTDETRTPRYELELSKIAVKNGELDVGVPAEVFFTLSQGKASLGDGTLEVRSSKKS
                     GWLVIEGTLGDITSMQGMPFFGSRFYLELAHTRDMETGRTLIEGQISGSPHDRNPAPF
                     EFEIDWNDYRLTMDLGIQKLIEQVIKNARPQGISIKSGVILSSRRFFDLIKKDYGDAE
                     ATGGGA"
     gene            complement(813..1934)
                     /locus_tag="PKK76_14835"
     CDS             complement(813..1934)
                     /locus_tag="PKK76_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69807.1"
                     /translation="MRRSLISLLAVAGLLSCHVDRPFEEPKPGDRIVHCNGRAYYLDA
                     ICEDEKEYLDWATKAYPEYPKFSNLTRDVLQKITVETKDVDKAASLFYERSLRDRNNI
                     EMMEFIGVKEKELAKRVPDYTGEKVLLALVPGMFWMDNPEVGAAGEQLRELADQMGMT
                     SSIVPVEQTGTVEKNGKDICEFLQQQTDVKSIIVASISKGGGDMKKALQYCGNTPAFR
                     KVRAWFNIAGITAGSQLVNGINDTIAYKAEARAYFCFKGYNWDGMMSMRAANDAPLEF
                     EVSVPKHIMVVNVIAVPLFRYVSSRAKPYYQYMIKYGPNDGITLLADSYVPGGSTYIS
                     FRNDHYFRNPISEQKMQAFLVYIMSKPFQAPPPVASASP"
     gene            complement(1934..3127)
                     /locus_tag="PKK76_14840"
     CDS             complement(1934..3127)
                     /locus_tag="PKK76_14840"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNL69808.1"
                     /translation="MFRFILGFSILLPAASLTAQADVPFLAEYKASYLQMSAKARANP
                     DSVGAQLMLTAPGPTVTTAFGHVALRVYTKLNDDTQDFYVDFGNYEESVAFVRKFLSG
                     SALFYTKAIPTTMAIDQWAVSGRGVITYKLVMTREEKARFLAAVEDAIKKTEGGYTYH
                     NFRQNCVTYIRDILEKTYATPIELNAAHLAEGDTWRSRVVKYSRAISWLQLGENYWFN
                     YLTDQKKNGRDVVYLPYDLYQAVVDGGHINGDRLVLKDRWAVRFDRQDWSGWLFIVVF
                     AIGFLLQLAPGSWGTRGRASVFIFSGFTGLVVWVVTLFTEYDFMARNALFFAVCPFDF
                     ALLSNRVMEKIRPWLKPFAFARVGLWILALVICLILPQKSFTALALSGLAWSCFLISV
                     LRGER"
     gene            complement(3223..>3571)
                     /locus_tag="PKK76_14845"
     CDS             complement(3223..>3571)
                     /locus_tag="PKK76_14845"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNL69809.1"
                     /translation="CSGGSGAPIVAAPGSSGMGGGSIMNNTKQNANCTLGAEDCIGET
                     YREQGTKGAVVGAKQGRSCANSILGLIASGDVSIKGAAAAGQITKITSVDQEDNSLLS
                     SVLVERCILVNGE"
BASE COUNT          934 a          875 c          901 g          861 t
ORIGIN      
        1 ggccagtttc acatcgactt caccggaccg ggctggattt tccccctctt ctactttcgc
       61 gcgcgaaagt tctctttgat gcttcgcgga ggagaaaaaa gagatcgcgt atatctggaa
      121 gcggatcacg tacaattctg ggtttcgcct tttgatttgc tcagaggccg gttggtgctg
      181 tggcgaatgc gattgattca tcctgttctg gaatacgtaa acaggcagga ctcttcggag
      241 aaaaacaaac tactccccgc acccggaaga tttctaatca agagaggcag gattcgtcac
      301 ggacggatca aagtgacaga tgaaacgcgc accccgcgct atgaactgga gctcagcaaa
      361 attgcagtga agaacggaga actggatgtg ggtgtcccgg cggaagtatt cttcactcta
      421 tcacagggca aagcatccct gggagatggc actctggaag ttcgctccag caagaaaagt
      481 ggctggctgg tgattgaagg aactctcgga gatatcacaa gcatgcaggg catgcccttc
      541 ttcgggagca gattctatct ggaacttgcg cacacgcgtg acatggaaac agggcgaact
      601 ttgatagaag gtcagatatc aggatcgcct catgatcgca atcctgcgcc ttttgaattt
      661 gaaatcgatt ggaatgacta caggctaacc atggatctag gaatccaaaa actgatagag
      721 caagtcatca agaacgcaag accccaggga atcagcatca agagcggcgt gattttatcc
      781 agccgccgct tttttgatct gatcaagaaa gattatggag atgcggaagc aacaggcggt
      841 ggtgcctgaa acggcttgga catgatatag accaggaacg cttgcatctt ctgttcagaa
      901 atcggattgc gaaagtaatg atcgtttctg aatgaaatgt aggtagatcc tccaggaaca
      961 tagctatccg ccagcaaagt aattccatcg tttggaccgt acttgatcat gtattgatag
     1021 tatggttttg ctctggatga aacataccgg aacagaggaa ctgcgattac attgaccacc
     1081 atgatgtgtt tgggtacaga cacttcgaat tcaagaggcg catcattggc cgccctcata
     1141 gacatcattc cgtcccagtt ataacccttg aagcagaagt aggcccgagc ttccgctttg
     1201 taggcaattg tgtcgttgat tccgtttaca agctggcttc ccgctgtgat tccagcgatg
     1261 ttgaaccagg cgcgaacttt gcgaaacgca ggagtgttgc cgcagtattg cagagctttc
     1321 ttcatatccc cgccgccttt gctgatagaa gcgacgatga tgcttttgac gtctgtttgc
     1381 tgttgcaaga attcgcaaat gtctttgccg tttttttcca cggtcccggt ttgctcgact
     1441 ggaacgatgg aagacgtcat tcccatttga tctgccagtt cgcggagctg ttctccagcc
     1501 gcgcccactt caggattgtc catccagaac attccaggca cgagagcgag aaggactttt
     1561 tctccagtgt agtcagggac tcgcttcgca agttcctttt ccttcacacc aatgaactcc
     1621 atcatttcaa tgttgtttcg atcccgcagc gaacgctcgt aaaatagaga agccgccttg
     1681 tccacgtcct ttgtttccac ggtgatcttc tgcagaacgt ctctggtgag gttgctgaat
     1741 tttggatatt ctggatacgc tttggtggcc cagtccaggt attccttctc atcttcgcaa
     1801 attgcatcaa gataataggc tctgccgttg cagtgaacga tccgatctcc cggctttggt
     1861 tcttcaaacg gtctgtccac atgacaggac agcaatcctg cgacagcgag cagggaaatc
     1921 aaggaccggc gcatcaacgt tctcctctaa gcacagatat aagaaagcaa ctccaggcca
     1981 gaccggaaag tgccagtgca gtgaatgact tctgaggaag gatcaaacaa atcacaagag
     2041 caaggatcca caggccgacg cgcgcaaatg caaaaggttt aagccagggc cggatctttt
     2101 ccataacgcg attggatagt aaagcaaagt caaacggaca gacggcaaag aacaaagcat
     2161 tgcgtgccat gaaatcatat tcagtgaaga gagtcacgac ccagactaca agtcctgtga
     2221 agccgctgaa aataaataca gaagcacgac ctctcgtccc ccaggagcct ggagcaagct
     2281 gcagtaaaaa tccaattgca aaaactacta tgaagagcca gccggaccaa tcctggcgat
     2341 cgaatcgcac agcccagcga tccttcagaa ccagtctatc cccgttgata tgaccaccat
     2401 ccactactgc ctggtataga tcatacggaa gataaactac atctctgcca tttttctttt
     2461 gatcggtcaa ataattgaac cagtaatttt cgccgagctg cagccaggaa atggctctag
     2521 aatatttcac tacgcgagaa cgccatgtat ccccttctgc gagatgagca gcgttgagtt
     2581 caatgggggt cgcgtatgtt ttctccagaa tatcgcggat gtaggtcacg caattctgac
     2641 ggaagttgtg gtatgtgtaa ccgccttccg ttttcttgat cgcatcttcc actgccgcca
     2701 aaaaccgtgc tttctcttct ctggtcatga ccagtttgta tgtgatgact ccgcgaccgc
     2761 tcacagccca ctgatcgatg gccatggtag taggaattgc tttcgtatag aaaagcgcgc
     2821 tgcctgagag gaatttcctc acgaatgcaa cagactcctc atagtttcca aaatcaacgt
     2881 agaagtcttg agtgtcgtcg ttgagtttcg tgtaaaccct gagggcaaca tgaccgaatg
     2941 cagtggtcac agttggacct ggtgctgtga gcatgagctg agcgcccact gaatcaggat
     3001 tcgcgcgtgc cttcgcagac atctgcaaat aagaagcttt gtattctgcg agaaacggaa
     3061 cgtccgcctg cgccgtgagg gatgccgcgg gaagcaaaat agaaaaaccg aggatgaaac
     3121 ggaacaattt agatgatagc attgaaaggt gtaaaagtgt gaggagggaa gtgaaaagaa
     3181 aagccttgcc ggtcgccaga ggcgaccgac aagctagagt gattactctc cgttaacgag
     3241 aatgcaacgc tccacgagta cagagctcag gaggctgtta tcttcttggt caacagaagt
     3301 gatcttcgtg atttggcctg ctgctgcagc acctttgatg gacacgtcgc cagaagcgat
     3361 gaggcccagg atgctgttcg cgcaggaacg gccttgctta gcaccaacta cagcaccttt
     3421 agtgccttgt tcgcggtaag tttcaccgat gcagtcttct gcaccaaggg tgcagttagc
     3481 gttctgcttg gtgttgttca tgattgaacc gccacccatt ccagatgaac ctggagcagc
     3541 tacgatcggg gcgccgcttc cgccggaaca t
//
LOCUS       DAORFF010000423         3556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_16509_length_3556_cov_3.192802, whole genome shotgun sequence.
ACCESSION   DAORFF010000423 DAORFF010000000
VERSION     DAORFF010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16509_length_3556_cov_3.192802"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PKK76_14850"
     CDS             <1..526
                     /locus_tag="PKK76_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69810.1"
                     /translation="LSAKYDHIITVSDSTIEAQNADAQTLITLLHLRDIASKTNRTFS
                     IVSEMLDNRNRELAEVTGANDFIVSDRLISLMMSQISESRELSEVFSELFQPDGSEIY
                     LKPVTDYVTPGKPLNFYTVIEAARLRNETAIGYRVEAEGSDASKQYGVHVNPLKSAQV
                     VFGANDRIIVLAEN"
     gene            complement(504..1739)
                     /locus_tag="PKK76_14855"
     CDS             complement(504..1739)
                     /locus_tag="PKK76_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL69811.1"
                     /translation="MDALGAFRIPTFRNYLLAKLLFVLGAQVQIAAVRWQVYSLTKDP
                     LALAWIGLIEAIAFIAVALFGGHLADMIRPGKIILACLSILAGCSGCLLLLSGPGSFL
                     LVSVTPIYAVIAVSGAARGILSPALFAFMTALIPPRLYASSATWSSGSWQMAAVAGPA
                     IGGLLYGAAGPIVAYTADITLVLGAIVFAGISIRTAPEQTLHPVTEGLSERLTGGVRF
                     VFGNQLLLGAMSLDLFAVLFGGAAAMIPLFADRLETGAQGMGFLMSAPAAGAVAMSVG
                     LAFAPLGRRAGWVLLSATAGFGLCMIGFALSSTFVLSMVFLVCSGAMDQVSVVVRSTI
                     MQRFTPEHMRGRVSAVNSIFVGSSNEIGAFESGLMAKILGLVPSVLFGASMTLVTVSA
                     TTAMAKKLRELDLSSPQEQ"
     gene            1813..2166
                     /locus_tag="PKK76_14860"
     CDS             1813..2166
                     /locus_tag="PKK76_14860"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNL69812.1"
                     /translation="MKLKATAARLLVDMDERRRFHRISSQFSLEVHPSESGEGTSQNV
                     SATGLLFSQKSPSPIGTTLYLTIRQPGLSGDVSVKGRVVRCEPIGSQFQIAVQFVDVD
                     KETEDSILDLVKESG"
     gene            complement(2196..2672)
                     /locus_tag="PKK76_14865"
     CDS             complement(2196..2672)
                     /locus_tag="PKK76_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69813.1"
                     /translation="MRSMFLIAALFALASCAGTADKVSGGEEIEGWRTDQATGVDTFF
                     MRVPGRATNNAIEQDSPTMMKSTCIDSTKLQALDNIIRKMIGETVTGQSATLDGQSTG
                     VLITSVRGGLIRGTQMGQCAPVDKSKKYESCECIHYVTGKGLKKKFELAVEQAAKK"
     gene            complement(2720..>3556)
                     /locus_tag="PKK76_14870"
     CDS             complement(2720..>3556)
                     /locus_tag="PKK76_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNL69814.1"
                     /translation="PPNISYTANPAEVESGTLFVQAARSWELDRIYSRVKLYLQKANG
                     PILNIVKGFTGSKYGLILDDLEHFYELDPKRFVAIAGANYPEQVMERKLTGYEVAANN
                     PDLIDHICKLFSTGYVFTRPAVVSDDVRGVQLGGALKNIYALGIGLLDGYYEKSLGGN
                     TDNSLFHVSNRIFKEMKIIGSKLGGKPGTFEGLSGLTDLMMACFGQDSRDRQYGHDYI
                     YGKANPDQKTAGLFGVRSLPNLLPLNPEEYPVASAVHAVLVQKRDYDSVLNEIMSKLK
                     RF"
BASE COUNT          902 a          950 c          877 g          827 t
ORIGIN      
        1 gctctctgca aaatatgacc acatcatcac agtgagcgat tcaactatcg aagctcagaa
       61 tgcagatgca cagactctca tcactctgct tcatctgcgc gacatcgctt caaagacaaa
      121 tagaacgttt agcattgtaa gtgaaatgct ggacaatcgc aaccgcgaac tggctgaagt
      181 gacaggtgca aatgatttca tcgtaagcga tcgcttgatt agcctgatga tgtctcagat
      241 ttcagagagc agggagcttt cagaagtctt ctcggaactt ttccagccag atggatcgga
      301 gatctatctc aagcctgtca ctgactacgt tacaccgggc aagcctctca atttctacac
      361 agtgatcgaa gctgccagac tcagaaatga aacggcgatc ggttatagag tggaagcgga
      421 agggtccgat gcatccaaac aatacggagt gcatgtaaat cctctgaagt cggcgcaagt
      481 tgtctttggt gcaaacgaca gaatcattgt tcttgcggag aactgagatc gagttcgcgc
      541 aatttctttg ccatggccgt tgtggctgac acagtcacaa gggtcatgga tgcgccaaaa
      601 agcacgctcg gaaccagccc cagaattttc gccatcagtc cagactcaaa ggctccgatt
      661 tcgttggaag agccaacgaa gatactgttc acggcagata ctcttccgcg catgtgctcc
      721 ggagtgaacc tctgcatgat ggtggatcga actacaacgc ttacttgatc catcgcaccg
      781 ctgcaaacca gaaacaccat agacaaaaca aacgtgcttg aaagagcaaa tccaatcata
      841 cagagtccaa acccagccgt cgcactgaga agcacccagc ctgcgcgtct tcccagcgga
      901 gcaaacgcaa ggcctacgga catggccaca gcacccgcag ctggcgcgct catcaaaaag
      961 cccatgccct gcgctcccgt ctcaagtcga tctgcgaaaa gaggaatcat ggctgcggct
     1021 cctccgaaca acactgcaaa caaatccagg ctcatcgctc cgagcaaaag ctggttgcca
     1081 aagacaaatc gcactccccc tgtcagtctt tcgctgagtc cttccgttac aggatggagt
     1141 gtttgttccg gtgctgtgcg aatactgata cccgcaaaaa caatggcccc gagtacaaga
     1201 gtgatgtctg ccgtgtatgc gacgatggga ccggctgctc cgtacagcaa acctccaatg
     1261 gcgggaccgg ccactgcggc catctgccag ctaccactgc tccaggtcgc agagctcgca
     1321 tacaatctgg gtggaatgag ggcagtcatg aatgcgaaga gagcaggcga aaggattccg
     1381 cgagccgccc cgctcactgc gatgactgcg taaattggcg tcacgcttac gagcagaaac
     1441 gaaccaggac ctgaaagaag aaggaggcaa cctgagcaac cggcaaggat cgatagacag
     1501 gccagaatga ttttgcccgg gcgaatcata tctgccagat gcccaccaaa caaagccacg
     1561 gcgatgaacg cgatcgcctc tatgagtccg atccaggcca gggcaagcgg atcctttgta
     1621 agactgtaca cctgccatct caccgccgcg atttgaacct gcgcgcccag cacgaacaac
     1681 agcttcgcca gaagatagtt gcgaaacgtt ggaatgcgaa aggctcccag ggcgtccatt
     1741 ctaccaggga accggaaggc tccctggcgc cgaagaaaaa ttcaagtttc cgacatccgt
     1801 tttaaaaaag acttgaaact caaggcgact gccgcaagac tactggtcga tatggacgaa
     1861 cgcagaaggt ttcatagaat cagctctcag ttctcactgg aggttcatcc ttcagaatca
     1921 ggagagggca ccagccagaa tgtaagcgcg acgggtctgc tgttttcgca gaagtcacca
     1981 tcgcccattg ggaccactct gtatcttacc atccgccagc caggtctgtc cggggatgtg
     2041 agtgtgaagg gccgcgtcgt tcgttgcgaa ccaatcggta gccagtttca aattgcagtg
     2101 cagtttgtag acgtagacaa ggaaacggaa gacagcattc ttgacctcgt gaaagagtca
     2161 ggctaggccc tgggcccagc cgctcttctt ttagattact tcttcgctgc ctgttccaca
     2221 gccagctcaa acttcttttt caagcctttg ccagttacgt agtggatgca ctcacagctt
     2281 tcgtatttct ttgatttgtc cacaggggca cattgtccca tctgcgttcc acgaatcaag
     2341 ccacctctta cagaagtgat caatacgcct gtggattgtc cgtcgagcgt tgcagactga
     2401 ccggtgactg tttctccgat cattttgcga atgatgttgt cgagcgcctg cagcttcgtg
     2461 gaatcaatgc aagtggactt catcatggtg ggactgtcct gttcaattgc gttgttcgtt
     2521 gcacgaccgg gcactctcat gaagaacgtg tctactcctg tggcctggtc tgtacgccag
     2581 ccttcaattt cttctccgcc agaaactttg tctgcggttc cggcgcacga tgccagagca
     2641 aacagcgctg cgattagaaa catggatctc attttgttct cctgaatgta ttggacgaat
     2701 cttaactctg cgagttcggt taaaaacgtt tcagctttga catgatttca tttagaactg
     2761 aatcgtaatc cctcttctgc acaagaacag catgtactgc agaggcaacc ggatactcct
     2821 ctggattgag aggaagcagg ttgggtagag atcgcactcc gaatagaccg gccgttttct
     2881 gatctggatt ggcctttccg taaatgtaat cgtgcccata ctggcgatcg cgcgaatcct
     2941 ggccaaagca cgccatcatc aaatctgtga ggccggaaag gccctcaaat gttccaggct
     3001 ttccgcccag cttgcttcca atgattttca tttctttgaa gattcgattg gatacgtgga
     3061 ataaggagtt gtctgtgttt cccccgaggc tcttttcata atagccgtct agaagtccaa
     3121 ttccaagagc atagatattc ttcaatgctc caccgagttg aactcctctt acgtcatcag
     3181 aaaccacagc cgggcgagtg aaaacatatc cggtgctgaa cagtttgcaa atgtgatcga
     3241 tcaaatctgg attgttggcc gccacttcgt atcctgtgag ctttcgctcc atcacctgct
     3301 caggatagtt tgcgccagcg atcgccacaa acctcttcgg gtcgagttca tagaaatgtt
     3361 ccagatcgtc tagaatcagg ccgtattttg agcctgtaaa tcctttgaca atgttcagga
     3421 tgggcccgtt tgccttttgg agataaagct tcactcgcga atagattcga tcgagctccc
     3481 aggatctggc tgcctgcaca aacagagttc cggattccac ctccgccggg ttggccgtgt
     3541 aagagatgtt aggcgg
//
LOCUS       DAORFF010000424         3536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_16680_length_3536_cov_4.027578, whole genome shotgun sequence.
ACCESSION   DAORFF010000424 DAORFF010000000
VERSION     DAORFF010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16680_length_3536_cov_4.027578"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="PKK76_14875"
     CDS             complement(<1..1398)
                     /locus_tag="PKK76_14875"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL69815.1"
                     /translation="MRTLAFLIPLTLCIACAHTIEPSGPVILLSPGALDGDIPLSISG
                     PVEFYWSRLIAPEEFRLKDRPQPDSILQQMGPWKAIAPASGRATYRFHLRCEPMPHKL
                     GLWFPHQFTASRVFANGKLIAETGIIGKTLGEDVIDRSDTLTSFDVSGPELELIFHIS
                     NQDSYRGGMRGSLVIGKPEVVSRYAQQRMLIEEIVLGIILGVAVYHLFFYFMHRTEYS
                     FLFFSILCFFLAARMPFQGSKIYHILLGPISWESQLRFLAVITIGAPPIGAALLRGLF
                     PDLVRLRSVFAYSAVAAISLLSQAGDLNVVSIATMLYAIVVYSFSGAHILFTTYRATR
                     AGAAAIPMALGVGSLSMLGILAFVQNFRGRDGGYYALAAFLFFVIFQALALSRFFLGA
                     VRARAEIAVSLQESRQALSQQRDDLQTSLHDSLGGALTDLQIHAEREMLSASGPSHDV
                     LAGIYNRIVDAVKMFR"
     gene            complement(1407..2045)
                     /locus_tag="PKK76_14880"
     CDS             complement(1407..2045)
                     /locus_tag="PKK76_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNL69816.1"
                     /translation="MDMNLEKWNLGVIENDELFREEVIRRLGDLPAARSVRAWPSAEQ
                     FLRDPSHKGIEILFLDIMLSGITGIELVRILSEKVPEMRVVMLTNMNSDVMIFDSIKN
                     GALGYILKSEIGNLEETVDVIGRGGATITPTIALRVFSTFRRVAEPGPDLTDREKQVL
                     GLMVKGKSIHSVATFLGLSDHTVHGYVKSIYKKLNVHNRAELAARAQELSLT"
     gene            2258..>3536
                     /locus_tag="PKK76_14885"
     CDS             2258..>3536
                     /locus_tag="PKK76_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate cyclase"
                     /protein_id="HNL69817.1"
                     /translation="MLTFTVGLSLSVIGLLSLRRNENRPLLVTFSLFCFGIGILGLLL
                     AFRAIVLNLDILLLLNRALYFGVVCLSPGAYMFAYFLTAKRYKPLLVMGIFAAASAVF
                     GWFGLITGYDFTGSWFHYSFGNYPIAQLPLKLWGLIAALGYFVAWIPATVRYFRTHPQ
                     EAATKKALFFSMHMISLLVISNLPSLAGIPVFPMSTFAFLPMLVLGYSIFRGDFLNVN
                     DLLFRKRLLFKFLAGLLTFGFIGISVLVAVTLPPATHSAIYERPFFLLPLFSAVCAFA
                     LAIFLAGSNPDQKLNMLAAASLMLAGTFMLVMTSFKLNLPLIVTRRIEQILYTAFLFT
                     PGVHLRFVYLALGRKSPRAVRLVDLVSALLCVVLWTPYFFDGFHEYSFGRISAGGPGL
                     NAYGALGLFAAGAFLFSWWKVRKEAQSKLGSLVV"
BASE COUNT          816 a          904 c          897 g          919 t
ORIGIN      
        1 acgaaacatt ttcaccgcgt ctacaattcg gttgtagatt cccgccagca cgtcatggga
       61 tggacccgag gcagaaagca tttctcgttc tgcatggatt tgcaaatcag tcagtgctcc
      121 tccgagacta tcatgcagag aggtttgcag atcatcgcgt tgctgcgaaa gcgcctgacg
      181 gctttcctgg aggctcactg cgatttcagc acgcgcacgc actgcgccca ggaagaaacg
      241 gctgagggcc agagcctgga aaattacaaa gaacaagaac gcggccaggg cgtagtagcc
      301 gccgtcgcgt cctctgaaat tttgcacgaa cgcaaggatg cctaacatcg agaggctgcc
      361 tacgccaagt gccatgggga tggcagcagc gccggcccga gtggccctgt acgttgtgaa
      421 gagaatgtgc gcacctgaga aggagtagac gacaatcgca tacagcatgg ttgcgatgga
      481 caccacattc aaatcacctg cctgcgatag aagcgaaatg gcagccacag cggaatatgc
      541 gaagactgaa cgcagacgaa ccagatcggg aaataatcct cgaaggaggg ctgcgccgat
      601 gggtggtgcg cctatggtga tgacagcgag aaatcgtaac tgactttccc aggatatggg
      661 tccaagtaga atgtgataga tctttgatcc ttgaaagggc attcgcgcag ctagaaagaa
      721 gcagagaatg gaaaagaaca gaaatgaata ctccgtgcga tgcatgaaat agaagaacaa
      781 atggtagacc gcaacgccca ggatgattcc cagtacaatc tcctcaatga gcatgcgttg
      841 ttgcgcatag cgcgacacga cttctggttt tccaatcacc agggaacctc gcattcctcc
      901 tctgtagctg tcctgattgc taatgtgaaa gatgagttcc agctcgggcc cactcacatc
      961 aaaagaagta agagtatccg atcgatcgat cacgtcctca ccgagtgtct tgccaatgat
     1021 tccagtttcc gcgatgagtt ttccattcgc aaatactcga gacgcggtaa actgatgcgg
     1081 aaaccacaga ccgagtttgt gaggcattgg ttcgcagcgt agatgaaacc tgtaggtggc
     1141 acgtcctgat gcgggagcga ttgccttcca cgggcccatc tgttgaagga tggagtctgg
     1201 ttgaggacga tcctttaagc gaaactcttc tggtgcgatg agtctgctcc agtagaactc
     1261 cactggaccg gaaatcgaca atggaatgtc cccgtcgagt gctcctggag aaagcaggat
     1321 gacgggtccg gaaggttcga ttgtgtgcgc gcacgcaatg catagtgtga gcggaatgag
     1381 gaaagcaaga gtgcgcaatg aatgtctcag gttagagaga gttcctgcgc acgggcagcc
     1441 agttccgcgc gattgtgcac attgagtttt ttgtaaatgg acttaacgta gccgtgaact
     1501 gtatgatcgc tgagcccgag aaacgtagcc acagaatgaa tgctctttcc tttgaccatc
     1561 agtccaagca cctgcttctc tcgatctgtt aaatcgggac cgggttctgc tacacggcga
     1621 aacgtggaga acactcgaag tgcgatcgta ggagtgatcg tagctcctcc gcgaccaatg
     1681 acatccactg tttcctctag attgccaatt tctgatttta gaatgtagcc aagcgccccg
     1741 ttctttatgg aatcgaaaat catcacatct gaattcatat tggttagcat gaccacgcgc
     1801 atttcaggga ccttctctga aaggatcctc acgagttcaa ttccagtgat cccggacagc
     1861 atgatgtcta gaaagaggat ttcaatccct ttgtgggaag ggtcccgcag aaattgctcc
     1921 gcagaaggcc acgcacgaac ggaacgtgct gcgggcagat ctccaagccg gcgaatcact
     1981 tcttctcgaa agagctcgtc gttttcgatt actccgaggt tccacttttc caaattcatg
     2041 tccatacaca gtactggtgt gacctgacgg agatcaaaca aaaagaaaac cgcaaccccc
     2101 ctgaacaggg ggtttttgaa taaaaaggat ctgctcttgc ttgactgctg tttgtgaacg
     2161 ttagacgccc tacacgatgc tacccttgcc gccactgtct gatcatcctg gggccgtcga
     2221 gtatttctgg taccaattgc cgtgggccgt tcctggtatg cttacgttta cggtcggttt
     2281 gtctctctct gtgattggac tcctttccct gcgtcgcaac gagaatcgtc cacttctggt
     2341 gaccttcagc ttattctgtt ttggcatagg gattctagga ttgcttctgg cttttcgcgc
     2401 catcgttttg aatctagaca ttctccttct gctcaatcgt gccctgtact tcggcgtagt
     2461 gtgcctttct ccgggtgcgt acatgttcgc gtactttcta accgcaaaga gatacaaacc
     2521 acttcttgtc atgggcattt tcgccgcggc atctgctgtg tttggctggt tcgggttgat
     2581 cacaggatac gattttaccg ggtcctggtt tcattattcg tttgggaatt atcccatcgc
     2641 tcaattgccg ctgaagctgt ggggcctgat tgcagcactt ggatatttcg tagcctggat
     2701 tccagccaca gtccgctact ttcgcacgca tccacaggag gccgcaacca agaaggcact
     2761 gttcttcagt atgcacatga tttctctgct cgtgatctcg aatctgccca gtcttgcggg
     2821 cattccagtt tttcccatga gcacatttgc atttttgcca atgctcgttc ttggctatag
     2881 catctttcgc ggtgactttc tcaatgtgaa cgatctactc tttcgcaaga gactgctgtt
     2941 caaatttctc gcgggccttc tcacgttcgg gtttattgga atctctgttc tagttgcagt
     3001 cactcttcct cctgcaacgc actcggccat atacgaacgc ccattttttc ttctgccgct
     3061 cttctctgcc gtttgcgcct tcgctctggc gatctttctg gcgggaagca atccagatca
     3121 gaaactcaac atgctggccg cagcttctct catgcttgca ggcacattca tgctcgtgat
     3181 gacttctttc aaactgaatc tgccattgat cgtaacacga cgcattgaac aaatcctata
     3241 cactgcattt ctttttacac ccggagtcca tcttcgcttt gtttatcttg cgctcggaag
     3301 aaagagcccg cgagcggtgc gactcgtgga cttagtctct gcgctgctat gcgtcgtact
     3361 ctggactccg tacttcttcg atggctttca tgaatacagc tttggtcgca tttctgccgg
     3421 aggtcctgga ctaaacgcgt atggtgcact cggattgttt gccgcaggtg catttctttt
     3481 tagctggtgg aaagtgcgga aagaagcgca gagcaaactc ggcagtctgg ttgttt
//
LOCUS       DAORFF010000425         3533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_16702_length_3533_cov_3.722829, whole genome shotgun sequence.
ACCESSION   DAORFF010000425 DAORFF010000000
VERSION     DAORFF010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16702_length_3533_cov_3.722829"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PKK76_14890"
     CDS             <1..307
                     /locus_tag="PKK76_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNL69818.1"
                     /translation="ENFNFTDDFRVNTADTGDPHFVKLRLSLGYEQGNPALTAELGQR
                     SPQLRNIINLILAGKTREDLRTVEDQLELREEIKASVNHVLAEGKVSEVYFNEFIVN"
     gene            307..975
                     /locus_tag="PKK76_14895"
     CDS             307..975
                     /locus_tag="PKK76_14895"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNL69819.1"
                     /translation="MRQITVYAHRGSALQARENTAAAFDSALHLGFKAFELDLLRLKD
                     GTIVVFHDFTLRRQFGKLKRIRSVTLKEFRKINSDLLTFDEFVDRYAKKKITVNFEVK
                     DDSKTLRAMLPGIAKFHDPVISSFRRTVVDAALCEGLEAGYLFHTMRAFRAGQKKMKG
                     NRLHISKNLLRSRNKPTKVFSSYNTHVYTVNDPIEALSLSAFPFVHGIFTDKPEVMHA
                     FQRK"
     gene            complement(1004..1768)
                     /locus_tag="PKK76_14900"
     CDS             complement(1004..1768)
                     /locus_tag="PKK76_14900"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNL69820.1"
                     /translation="MFFFLSKVLPGLLFPYPLFLILSGIVLRKMQPGRLRRLFGLALL
                     LVTVASTYVTAAFLMRGLENRYAPLTVEETPTAEAIVVLSGMVTPLTRAKGRPEFSAA
                     VDRILAGRELLAARKAPVLIISGGSGLISQIGTPEAETLRNWMIKDGFAPDKVIAEKA
                     SRNTAENAVETAKIARARGIKKIILVTSAFHMQRSVLCFEKEGFEITPYPVDYYILDE
                     FPGIEAFFPAPAGMTLSGIALKEYAGIIAYRLKGYL"
     gene            2498..3217
                     /locus_tag="PKK76_14905"
     CDS             2498..3217
                     /locus_tag="PKK76_14905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HNL69821.1"
                     /translation="MTKSGLTLPSAPRKDFRRNFIQVAVCELRFPIVLELESKPPARL
                     HSQIRQSYPNYEVRTSLTIEPQSETREPVYTFSSREKIWTIAIRPYAIALETRNYTSF
                     TDFMKRMKALIVPAAKEFDTEMYTRVGLRFIDHIPFNGDPVNSWVKEDLVRSVTDSHL
                     QPESFWQVIQGKASKGKYTYQHGIHPQQSGTYVLDFDFYKEDVDLDVTERLIKDLHEQ
                     SRDLFFWSLGQKSLDFMASEG"
BASE COUNT          928 a          854 c          846 g          905 t
ORIGIN      
        1 tgaaaatttc aatttcacag atgactttcg cgtcaacact gcagataccg gagatcctca
       61 cttcgtaaaa ctcagactga gtctgggata tgaacaagga aatccagcgc ttacagcaga
      121 acttggtcaa agaagtccac agttaagaaa tattatcaac ttgattcttg cgggcaagac
      181 gcgcgaagat cttcgcacag tagaagatca gcttgaattg cgcgaagaga tcaaagcctc
      241 cgtcaatcac gttctcgcgg aaggtaaagt ctccgaagtg tatttcaacg aattcattgt
      301 caactgatgc gtcagattac agtctacgct caccgcggca gcgcactaca ggcccgtgaa
      361 aacactgccg cggcatttga ctccgctctt catttaggtt tcaaagcgtt cgaactcgat
      421 ctgttgcgtc ttaaagacgg cacgatcgta gtctttcacg acttcactct gcgcagacag
      481 tttggtaaac tcaaacgaat tcgttctgtc actctcaaag aattcagaaa gatcaattct
      541 gatttgctta cgttcgatga gttcgtggat cgttatgcga aaaagaagat cacagtaaat
      601 tttgaagtaa aagacgattc taaaactctt cgcgccatgc ttccaggaat tgcgaaattt
      661 cacgatcctg tcatttcctc tttccggcgc actgtcgtgg atgctgccct ctgtgaaggg
      721 cttgaggccg ggtatctatt tcacactatg cgtgctttca gagccggtca gaagaagatg
      781 aagggcaatc gccttcatat ttcaaagaat ctccttcgct ctcgaaacaa gccaacaaag
      841 gttttttcct cttacaatac tcacgtgtac acggtcaatg atccaatcga ggctttgtct
      901 ctctcagcat ttccgtttgt tcacggaatc tttacagaca aaccagaagt catgcacgcc
      961 tttcagcgga aatagtcctt ctgttttgtg ccaggcgcgc atcctacaga taccctttca
     1021 ggcgataggc gatgatccct gcgtattctt tgagagcaat accgcttaac gtcattcctg
     1081 ccggggccgg aaagaacgct tcaatccctg ggaattcatc caggatgtag taatccactg
     1141 gatagggagt gatctcgaag ccctcttttt caaaacacag cacactgcgc tgcatgtgaa
     1201 aagcggaagt caccaggatg atttttttaa ttccacgagc tcttgcgatt ttggctgttt
     1261 caactgcgtt ttctgccgtg ttcctggacg ccttttctgc aatcactttg tccggtgcga
     1321 atccgtcttt gatcatccag tttcgcaacg tttctgcctc cggtgttccg atctgcgaaa
     1381 tcagccccga gccgccgctg atgataagaa caggcgcttt tcgcgcggca aggagctctc
     1441 ttcctgccag aatcctgtct acggcggcgg aaaactcagg tcttcctttc gcgcgcgtga
     1501 gcggcgtaac cattccggaa agaactacga tggcctccgc ggtgggtgtt tcctccactg
     1561 tcaacggcgc ataacggttt tcgaggccac gcatgagaaa cgcggcagtg acataagtag
     1621 atgctacggt gacaagcagc agtgccagtc cgaacaatcg ccgcaaacga cccggttgca
     1681 tcttcctcag aacgattcca gaaagaatca agaaaagcgg gtaaggaaac aaaaggcctg
     1741 gaagaacttt tgaaagaaag aaaaacattc agacaacctc ctgacgcacc attttgtgcg
     1801 atgcattgat ggcaatacag taataatact ttagaactac atactgcagt cgcttcggaa
     1861 gcaattctgt tgactgcgtc gcagttttct cgggcgcgac cttttttttc ttgcgtcaag
     1921 tatcgacgtc aaacccggga cttacgcgcc gcttcgagta taacggacag tgtagtgaag
     1981 agcggcaagg aggcaggcgg atttaagatc aacgaggtgg gctttgccta gcgggacttc
     2041 gcatttgaac ttgtgcctct gaagcttatg gaggagaaca aggacggcct caatctgggc
     2101 gcaccatcca tttgttttcg gacacctgat ttgtctaagc tgcgttctcg gctcatggat
     2161 tagaaggtaa cggtaactgg ataacagacc attgcggaag ttctctttcg cattccagga
     2221 caatgacgga aactggttcg ccgcaactaa tgtgccgcgc aggtgaacgt cctagtattg
     2281 tcgaaactgc tcatcagtcc acgaggtgac gccacagaat gtatattccg caacttggtt
     2341 ttgtggttga tcaacctaat ctacgacgat gcaagcttgc ggatgcctct tgtggatttc
     2401 ggtagctcat tttgacaatg aaatctgggt tcagaatatt tgagacagga gtttgatttt
     2461 ggttgctaaa ccccggacgg gccttaattc gagtcccgtg acaaaaagcg gtctaactct
     2521 cccaagtgcc ccaagaaagg atttcagacg taacttcatc caggtagcgg tatgcgagtt
     2581 gcgattccca attgttttgg agctggagtc gaagccacct gcacggttgc attctcagat
     2641 cagacaatca tacccaaact acgaagtccg cacgagtttg acgattgaac cgcaatcgga
     2701 aacaagagag cccgtgtaca cattttcatc ccgcgaaaaa atatggacca tagcgatacg
     2761 tccctacgcc attgcactcg aaactcgcaa ctatacttct ttcaccgatt tcatgaaacg
     2821 gatgaaggct ttgatagttc cggcggccaa agagtttgac acggaaatgt acactcgggt
     2881 cgggctgagg ttcattgatc acattccttt caatggtgat ccagttaatt cctgggtcaa
     2941 agaagacctc gttcgttccg taacagatag tcatcttcag cccgaatcct tttggcaagt
     3001 aattcaagga aaagcctcga aggggaagta cacctatcag catggaattc atccgcagca
     3061 atccgggact tacgttcttg acttcgattt ctacaaagag gacgtggatc tggacgttac
     3121 agagcgatta ataaaggact tgcacgaaca gagtcgggat ctcttttttt ggtcgctggg
     3181 tcagaaaagc ttggacttca tggcatcaga agggtagccg agtgtttcca atcgacgtat
     3241 catcatatat tacagatcga gaaccgcaga ggttcgaact tacagagagg caatttccgg
     3301 taaggagaga tcgtccgatt tgggaaactg ttaccggtgg tcaagccttc cagagctttt
     3361 caacaccgat ggtgacggga gcagcctatg ggcgggtcgt tgattcaacg cacaacgttt
     3421 tagaaagttt accggatcaa agggtcgaac tcttggttag acagttttca ggcggcctat
     3481 cgagagagga atttcgtaga ctcgaactgc taaatcagcg cctgacgaaa ctt
//
LOCUS       DAORFF010000426         3528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_16732_length_3528_cov_3.230636, whole genome shotgun sequence.
ACCESSION   DAORFF010000426 DAORFF010000000
VERSION     DAORFF010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16732_length_3528_cov_3.230636"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKK76_14910"
     CDS             complement(<1..202)
                     /locus_tag="PKK76_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69822.1"
                     /translation="MRFSLVAAVFFLASALFADTVILKNGQTIFGNIVGQTRTDVQIN
                     VNGQVITLQKEAIRRIQYGDNRA"
     gene            283..1725
                     /locus_tag="PKK76_14915"
     CDS             283..1725
                     /locus_tag="PKK76_14915"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase family protein"
                     /protein_id="HNL69823.1"
                     /translation="MLEEILKNIEVFSALDSASLSTVAARMKQIQLKAGDVICNEGEP
                     GHSMFVLESGELSVQKRGKDKTLVEIARLRPGEVAGIMSLFGDESRSATLISTGEARL
                     WEISNEDFQALVQSTPQIAHQLLKVLSRYLREETRIVAELRTRDVENRLKIAFFDSKP
                     YTEEAFKDKNEDKYALQFFESRLSMETLVMAEGFKIVCAFVNDTLDAPVVKKLAAMGI
                     EMIAMRCAGYNNVDLAACKEHGITVANVPAYSPHAVAEHAVALMLSLNRHITRAHSRV
                     REGNFSLNGLVGFDMYGKTAGVIGAGKIGQCVISILAGFGCKVLVYDKFPREQTIPNV
                     RNASLDEIFTQSDIITLHAPLIPETRHIISRESISKMKRGVMIINTSRGGLLDAHALV
                     DGLVSGQVGSAGLDVYEEEKDYFFEDYSDMILKDDVLARLTTFHNVIVTGHMAFLTRE
                     ALLNIADVTLNNVLEFENGLRMGSMKNGLA"
     gene            complement(1727..2980)
                     /locus_tag="PKK76_14920"
     CDS             complement(1727..2980)
                     /locus_tag="PKK76_14920"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNL69824.1"
                     /translation="MTGSGIFRFCCGLVFCASVLFLSACSSQVDWIEYRGKNGSGGTA
                     NALHPPLGLRWKLRLHDETGKEAKAFNPPVVIGDMMYFGSIDGNFYALDTETGYMKWN
                     FKTRGAVNSVPFADDQAVYFGSNDGSVYAVDLKDGKQRWSFPTGNTVQSLVLRYKDNI
                     IFTSDTGATFFLDLNGHEQQRLPNPVWSHHTFQVYEGVVYWAPRGRSFGAYDMATKQF
                     LWSVDVTVPYAVWYSFPAIDSKQIYYSSSYFVGGDDPVKLTFYAHDRLTGEATWQNED
                     SIDMGGTPPTRETVFMRHVQLLDYLAPALYKDLVIYTSGDSVVRAFDTKTGSPAWRKN
                     LPYATSSAVTVAGDRIYFGMRGDEAVEGVTGKKPKLICMSARDGNILWQMDTEGAILS
                     SPVISGKRIMFGTDKNYFYVLEEIF"
     gene            complement(2977..>3528)
                     /locus_tag="PKK76_14925"
     CDS             complement(2977..>3528)
                     /locus_tag="PKK76_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69825.1"
                     /translation="PRYGWVDFETTSFAIPPQPKMDPNNQDVLIPLIEEVPQVREQKF
                     KFPYHFAGRVLAILTASILAALYLYRFFRLMYLKAVARSMTTRGLDALFRVIIIRMAR
                     EGYDVRPPFLTHAEYARIYPQLGAFAAVFTALRFREHYDEESKIRDWNVLRAESADVM
                     KRIRKPGAWAGIRRLLTLNGIWI"
BASE COUNT          944 a          879 c          868 g          837 t
ORIGIN      
        1 ccgccctgtt gtcgccgtat tgaattcgtc ggattgcttc cttctgcaaa gtgatgacct
       61 gaccgttcac attgatctgc acatccgtgc gagtctggcc tacgatattt ccaaaaatcg
      121 tctggccatt ctttagaatg acggtatccg caaagagggc ggaagcgaga aagaaaacgg
      181 ctgcgacaag agaaaagcgc atgtctacgg ttgagccagg tgggttcagc cgcaatcgaa
      241 aatacttctt gtttgcgagc gggcccctgg ttgtatcgaa cgatgctcga agaaattctg
      301 aaaaatatcg aagtcttttc cgcacttgat tctgcgtctc tttccacagt tgccgcacgc
      361 atgaaacaga ttcaattgaa agcgggagac gtgatctgca atgaaggaga gcccggtcac
      421 agcatgtttg ttctggaatc gggggaactg agcgtacaga aaaggggcaa agacaaaacg
      481 ctcgtcgaga tcgcgcgctt gagacccggc gaagttgcgg ggatcatgag tctatttggc
      541 gacgaaagta gatcagccac tctgatttct acaggagagg cacggctctg ggaaatcagc
      601 aatgaagact tccaggctct cgtgcaatct acgccgcaaa tagcgcacca gcttctcaaa
      661 gtgctcagca gatatctgcg cgaagaaacg cgcatcgtag cagaactgcg tacgagagat
      721 gtagaaaata gactgaagat cgcctttttt gattcaaagc catatacaga agaagcattc
      781 aaggacaaaa acgaagacaa gtatgcactt cagttctttg aatctcgtct cagcatggag
      841 accctggtca tggcggaagg attcaagatt gtatgtgcat ttgtaaacga tactctagac
      901 gcacccgtgg taaaaaaact cgcagccatg ggaattgaaa tgattgcaat gagatgcgcg
      961 ggctacaaca acgtggatct ggctgcgtgc aaggaacacg gcatcactgt ggccaatgtt
     1021 ccagcctatt cccctcatgc agtggcagag cacgcagtgg ctctgatgct ttctctcaac
     1081 cgccacatca cgcgtgcaca cagtcgggtg cgcgagggca acttttcact caatggcctg
     1141 gtcggttttg atatgtatgg aaagacagca ggtgtaattg gtgcgggtaa gattggccag
     1201 tgcgtgattt cgattctcgc tggatttggg tgtaaggtgc ttgtatacga caaattccct
     1261 cgcgaacaga caataccgaa tgtgcgaaac gcaagcctcg acgaaatttt cacacagtcg
     1321 gatatcatca ctctacacgc gcctctgatt ccggaaacgc gccacatcat cagccgggaa
     1381 tccatttcga aaatgaaacg cggggtcatg atcatcaata caagccgcgg cggacttctg
     1441 gatgctcatg ccctggtaga tggtcttgtc agcggacaag tcggaagcgc gggcctggat
     1501 gtatacgaag aagagaaaga ctatttcttt gaagactact cagacatgat tcttaaagac
     1561 gacgttctgg cgcggctgac cacttttcac aatgtgatcg tgaccgggca catggccttt
     1621 ctcacgcgcg aagcgcttct caacatcgca gacgtaacgc tgaacaacgt gctcgaattt
     1681 gaaaatggtc taagaatggg ctctatgaaa aacggactgg cttgatttaa aaaatctctt
     1741 ctagaacgta aaagtagttc ttgtccgttc caaacatgat tctttttccc gaaattacag
     1801 gggagctgag aatggctccc tctgtatcca tttgccagag aatatttccg tcgcgagcag
     1861 acatacagat aagctttggt ttttttccag tgactccttc caccgcctca tcgccgcgca
     1921 ttccaaaata gattctatcc ccggctacag tcacagcaga acttgtcgca tagggtagat
     1981 tctttctcca ggccggactt ccagtcttcg tatcaaacgc gcgaaccacg gagtcgccgc
     2041 ttgtgtagat caccagatcc ttgtagagag ctggcgcaag atagtcgagc agctgcacat
     2101 gccgcataaa tacagtttcg cgcgtgggag gagttcctcc catatcaatg gagtcttcat
     2161 tctgccaggt tgcctctccc gtcaggcgat catgagcgta aaacgtgagc ttcacaggat
     2221 cgtctccgcc cacaaaataa ctactcgaat agtagatctg ctttgaatcg atcgccggaa
     2281 aggaatacca tacagcgtaa ggaaccgtta catctacgct ccacaaaaat tgcttcgtgg
     2341 ccatgtcata ggcgccaaag ctccgtccgc gcggtgccca gtagacaaca ccctcataga
     2401 cctgaaacgt gtgatgggac cagactggat tgggcaacct ttgctgttcg tgaccgttta
     2461 aatccagaaa gaaggtggca cctgtatcac ttgtaaaaat gatattatct ttgtatcgaa
     2521 gcacgagaga ctggactgta tttccggttg gaaaactcca gcgctgcttt ccatccttga
     2581 ggtccacggc gtagacgcta ccgtcgttgg atccaaagta aactgcctgg tcatctgcaa
     2641 aaggaactga gttcacagca ccgcgcgttt tgaaattcca cttcatgtac cctgtctctg
     2701 tatccagagc gtagaagttc ccatcaatgg aaccaaagta catcatgtct ccgatcacca
     2761 cgggaggatt gaaagctttt gcttcttttc cggtctcatc atgcaatcgc agtttccatc
     2821 gcaggcccaa tggaggatga agtgcgttgg ccgtgcctcc tgaaccattt ttccccctgt
     2881 attcgatcca gtctacctgg ctcgaacagg ctgacagaaa cagaacggat gcgcaaaaaa
     2941 ctagaccgca gcagaagcga aagattccag acccggtcat atccagatcc cgttgagagt
     3001 caaaagacga cgaatgccgg cccaggctcc cggctttctg attcgtttca tgacgtctgc
     3061 agattcagcg cgaagtacat tccaatcacg aatcttactc tcttcatcat agtgctcgcg
     3121 aaaacgaagt gcggtgaaga ccgccgcaaa tgcacccagt tgcgggtaga tgcgcgcata
     3181 ctctgcatga gttaaaaatg gtgggcgaac atcatagcct tctcttgcca tgcgaatgat
     3241 gatcacgcga aagagtgcgt ccagacctct tgtggtcata gaacgagcca ctgccttcaa
     3301 atacatcaac cgaaagaatc tgtacaaata gagagcagca agaatgctcg ccgtgagaat
     3361 cgcgagaact cgccctgcaa aatggtatgg aaacttaaac ttttgttctc taacctgagg
     3421 cacttcttca atcagcggga tcagaacatc ctgattgttt ggatccattt tgggttgcgg
     3481 cgggatggca aaagacgtag tttcgaaatc tacccatccg tatctggg
//
LOCUS       DAORFF010000427         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_16801_length_3520_cov_3.421068, whole genome shotgun sequence.
ACCESSION   DAORFF010000427 DAORFF010000000
VERSION     DAORFF010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16801_length_3520_cov_3.421068"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PKK76_14930"
     CDS             <1..514
                     /locus_tag="PKK76_14930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNL69826.1"
                     /translation="IEDMLIISKVESRNVPYQPARVDLESYMENLRMLVAGILEQKQQ
                     NLEIILQQKTIVADPLLLEHLLLNLIDNASRYSNASTKITVSVSRTGNNAAIEVTDEG
                     PGIPSQIREKIFERFFRADQNRSRREGGSGLGLSIVRQIARIHGGNVTVQDGPQGGSV
                     FRVLLPQTPD"
     gene            562..1401
                     /locus_tag="PKK76_14935"
     CDS             562..1401
                     /locus_tag="PKK76_14935"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNL69827.1"
                     /translation="MQMRILATFLLIFMIQCSQKKNAEFCSVGGEELSPIVDSWSAIY
                     QESGRKVPQHEARGNRTAAPALASRICNLAFVSDALPSEDLDLITRKLGRTPGAIPIA
                     VEIIAFAVPKSFPVDSLSVEQIKSIFGSGEEDASALGLPPGKISAFGINSASDRYFWL
                     KGALGIRAFSDRVKEMSGPLTLMDRVAVTSYGIGYGRPLEANGVKLLDISKDGRVLGI
                     KSTDYPFRRYYFAYVTEKTPDTKAFLEFVLSDRGQKALLPSGLYPLPDSVLIKAREEL
                     ARQ"
     gene            1398..2552
                     /locus_tag="PKK76_14940"
     CDS             1398..2552
                     /locus_tag="PKK76_14940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL69828.1"
                     /translation="MILTRPIILLSLVSLFTDIASEMLYPVMPIYLGSIGFSVFYIGV
                     LEGFAEATAGLSKGYFGNLSDAIGRRLPFVRGGYALSAISKPMMAVSVFPGWIFLSRT
                     VDRLGKGIRTAARDAMLSDASAPEHRGRVFGFHRALDTTGALLGPVLSLTFLYYLPGQ
                     YRTLFILAFIPGAFAILLTLFLSEIRGNSRTRPGILDFLKYAIHGPRSYRKLIAGLLF
                     FALMNSSDAFLILLMKQRGVSDSAAILAYIFYNTVFALAAYPAGMAADRFGAKRMLTI
                     GLLLFAFVYAAMPYAHSPITFALLLAMYGIYAAATEGIGKAWITTTAAPEDVATAVGT
                     FASLQSICTICASAAAGALWLWAGPEIVFGISAAGTFIAAMYFVFAFRTA"
     gene            2568..>3520
                     /locus_tag="PKK76_14945"
     CDS             2568..>3520
                     /locus_tag="PKK76_14945"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL69829.1"
                     /translation="MRAWLSIFGLLLAASAIHGDAVTVPRTGFAEVEAGKVFILSDPG
                     VDSVEEITKREKDFKPATTLDLLDPGKVYWAYFDIVNPDPGMPVLEARSDLLHRIDLY
                     FEQDGRMVQKSAGISLPFMQREIAYRYSAFRLPMKPTIRVYLRLEFQIRQHMSLKLWN
                     EKWFTQKNRREQLILGVYYGIVIAMAVYNLFVFLSLRSVSYMHYVLYIISFALYQTTL
                     NGISFEYFWPQTAGLDIKASFVSAGLSIMFAALFARHFLGVEKRSRFFNFAFLGSAAL
                     AGLWAAASLILPFQTANVMGRVLGSLVVPLIIAGSIWALRDG"
BASE COUNT          812 a          922 c          875 g          911 t
ORIGIN      
        1 catcgaggat atgctcatca tttctaaagt ggaatcaaga aacgttcctt accagcctgc
       61 ccgggtggat ttggaatcgt atatggaaaa tcttcgcatg ctcgtggcgg ggatactgga
      121 gcaaaaacag cagaatctgg aaatcattct gcaacaaaaa accatcgtcg cagaccctct
      181 gctcctggag catttgctct tgaatctcat agacaatgcg tctaggtatt caaacgcatc
      241 tacgaagatc accgtttcag tgtcgcgtac tggaaacaac gcagccattg aagtgacaga
      301 cgaaggccct ggcattccgt cccagattag agagaaaatc tttgaacgat tctttcgcgc
      361 agaccagaac agatcgcgca gagaaggtgg atccggactc ggcctgagca ttgtcagaca
      421 aattgctcgc attcacggcg gaaatgttac agtgcaggat ggccctcagg gcggatctgt
      481 gtttcgtgtt ctgttgccgc aaacgcctga ttgaaatgct ttcggcttga cccggacggc
      541 cggcaccact ggatctactc gatgcaaatg cgaatcctgg ccaccttcct tctgatcttc
      601 atgattcagt gttcccagaa aaagaacgct gaattctgca gtgtgggtgg agaagaactc
      661 agtcctatcg tagactcctg gtctgccatc tatcaagaat caggaagaaa agttccacag
      721 cacgaggcgc gcggaaatag aacggccgct cctgcccttg cttcgcgcat atgcaatctt
      781 gcctttgtgt ccgacgcact cccttcagag gacctggatc taatcacgcg gaagctcggt
      841 cgcacaccgg gcgccattcc aatcgcagtg gagatcatcg cttttgcagt gcccaaatcc
      901 tttccggtgg actctctaag cgtagaacag atcaaatcga ttttcggatc aggcgaagag
      961 gatgcgagcg cacttggcct tccgcccgga aaaatttctg cgttcggaat caactcagct
     1021 tcagacaggt acttctggct caaaggtgcc ctggggattc gtgctttttc tgatagagta
     1081 aaagaaatgt cagggccact gacgctgatg gatcgagttg cggtcacgtc atacggcatt
     1141 ggttatggaa ggcctctgga agcaaatgga gtgaagctac tggacatcag caaagacggg
     1201 cgagtgctgg gaatcaaatc aacggactat ccttttcgca gatactattt cgcgtacgta
     1261 acagagaaga ctccggacac aaaagcgttt ctggaattcg ttctatccga tcgtggacaa
     1321 aaagcgctcc tcccctcggg actctatcct cttcccgatt ccgttctcat caaagcgagg
     1381 gaagaactgg cgcgtcagtg atcctcacgc gccccatcat ccttctttcg cttgtgagtc
     1441 tttttacgga catcgcaagc gaaatgctct atccggtcat gcccatctat ctgggatcga
     1501 ttggtttttc cgtattctac attggagtgc tcgagggatt cgcagaagca acggcaggac
     1561 tatccaaagg atactttggc aatctatcag atgccattgg ccgtagactc ccgtttgtca
     1621 gaggaggata cgcgctcagc gcgatttcca aaccaatgat ggctgtttct gtttttccag
     1681 gctggatctt tttatcacgc accgtagaca gactcggaaa aggaattcgc actgccgcca
     1741 gagatgcgat gctttcggat gcatctgccc cagaacacag aggaagagtg ttcgggtttc
     1801 acagagccct ggatacgaca ggagccttgc tcggtcccgt gctatcactt acatttcttt
     1861 actatttacc aggacagtat cggacgctct tcattcttgc cttcatccct ggagccttcg
     1921 ccattctcct tactctgttc ctttcggaaa tccgcggaaa ctctcgaacg cgtccaggga
     1981 tcctggattt tttgaaatac gccatccacg gtccccgatc ctatcgcaag ctcatcgcag
     2041 ggctactgtt cttcgcattg atgaatagtt cggacgcatt tctcattctc ctcatgaagc
     2101 aaagaggggt gagcgattct gccgctatac ttgcctatat tttctacaat actgtatttg
     2161 ccctggccgc ctatccagca gggatggcag ctgatcgctt tggcgcaaag cgaatgctga
     2221 cgataggact ccttcttttt gcatttgttt acgcggccat gccctatgcg cactcaccaa
     2281 tcacctttgc gttgttactc gcaatgtacg gtatctacgc cgccgcgaca gaaggaattg
     2341 ggaaagcctg gatcacgact acagcggcac ccgaagatgt ggccacggct gtaggcacgt
     2401 ttgcctcttt gcagagtatc tgcacaattt gcgcaagcgc cgcggccggc gctctgtggc
     2461 tctgggccgg gccggagatc gtatttggta tatccgcggc aggcactttt atagccgcca
     2521 tgtacttcgt gtttgcattc cgtactgctt gatccatttg ggtcttaatg cgcgcctggc
     2581 tttccatttt tgggttacta ttggccgcgt ccgccattca cggagatgct gtgacggttc
     2641 cgcgcactgg atttgctgag gtggaggcgg ggaaagtatt cattctctcc gatccaggtg
     2701 tggactccgt ggaagaaatc acgaaaagag agaaagactt caaaccggcc acaactctgg
     2761 acctgctcga tccggggaaa gtatactggg cctactttga tattgtaaac ccggacccgg
     2821 gaatgcccgt gctggaggca agatctgatc tgctgcatcg tattgatctg tacttcgaac
     2881 aggacggacg aatggttcag aaatcggccg ggatcagcct cccattcatg cagagggaaa
     2941 tcgcctatcg ttattctgca tttagactcc cgatgaaacc aacaattcgc gtgtacctca
     3001 gattagaatt ccagattcgg cagcacatgt ctttgaaact gtggaatgaa aagtggttca
     3061 cccagaagaa caggcgcgaa cagctcatcc tgggtgttta ctatggcatt gtcattgcca
     3121 tggcagtgta taacctcttt gtatttctct cactgcggtc tgtgagttac atgcactatg
     3181 tgctctatat catttcattc gcactgtatc agacaacgtt aaacggcata tcattcgagt
     3241 acttctggcc acaaactgca ggactcgaca tcaaagcctc cttcgtttcc gccggtctct
     3301 ccatcatgtt cgcagcactc ttcgcgcgac attttcttgg tgtagaaaaa agatctcgct
     3361 ttttcaattt tgcatttctg ggttccgccg cattggcagg gctctgggcc gccgccagtt
     3421 tgatccttcc tttccagact gccaatgtga tgggaagagt cctcggaagc ctggtagttc
     3481 ctcttatcat cgcaggttcc atctgggcgc tgcgcgatgg
//
LOCUS       DAORFF010000428         3511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_16876_length_3511_cov_3.365741, whole genome shotgun sequence.
ACCESSION   DAORFF010000428 DAORFF010000000
VERSION     DAORFF010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16876_length_3511_cov_3.365741"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PKK76_14950"
     CDS             <1..752
                     /locus_tag="PKK76_14950"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNL69830.1"
                     /translation="FQSDWSRGGDAIIVDRSIHMPEELKGKRVAFLPASPSHTLLLHV
                     LDTAKLNYRDLQLVETTSARDAATLFKNGKVDAAVVWSPDDEDCESGARRCVKSVAGS
                     RILSSTTHAPDIIADVFFAKKSYIEKHRDALRYLIQGWMIGNAEINESVEARSRAARI
                     LSVGMRQPVGFCAEAMKKVRLVTYGDNRIFFGLDKRSTNVTAEDLYTRMAARYKSLHL
                     APENAPAWKQIVDTSILSTVTGLTGNQSPEN"
     gene            810..1301
                     /locus_tag="PKK76_14955"
     CDS             810..1301
                     /locus_tag="PKK76_14955"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNL69831.1"
                     /translation="MGKPVVHFEIASANSTEAQTFYSKLFGWEIDANNPMKYGIVKTA
                     KGVGINGGLFQADAGKEAVVVYVAVEDTDAYLKKVVSMGGTVIHPTEVIPGMVTFALF
                     ADPFGTVMGLIKDVPPPKPAPAIAAGKKANPAAKTKAVKKAAPKKKAKKAAKKPAAKK
                     KRK"
     gene            complement(1332..1883)
                     /gene="def"
                     /locus_tag="PKK76_14960"
     CDS             complement(1332..1883)
                     /gene="def"
                     /locus_tag="PKK76_14960"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNL69832.1"
                     /translation="MAVRKILRIGHPELRKKSEPVPVTEIRSAEIKKLIRDMFDTMAE
                     AGGIGLAAPQVGVHKRIVIVGFDRSDRYPELKQGIERRILVNPEIEILKAPPVGFWEG
                     CLSVPDMRGYVERVRKIRLKFYDENEEFHDEVIEGFDAVVYQHECDHLDGVLYVDRLK
                     DPKKFGYNDELDLLEANRTKVIV"
     gene            complement(1919..2704)
                     /locus_tag="PKK76_14965"
     CDS             complement(1919..2704)
                     /locus_tag="PKK76_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL69833.1"
                     /translation="MTPDSEEIISGERPPLLLIHDVFSDTDAWAEQIDFLSRQYDVLN
                     FPLSGHRGAQGSSTSSLLEDNRSRLDQIVGSVEMISIIAHGHSCRLAVDLASDFPDRV
                     AQLILISPVLNPLNLMRGLPRFMPFFYEIFFLFADTLGEKGPSFHRPVARLASISAPA
                     MRQWVEEWRALPAPDLTRVQARTLILSGDRMPRVPDPPVGDINENLTNSHLIRFAGMG
                     HFLQKENAATINPLLMDFLNRPEGYLQRGLESVVGFIKGLFGR"
     gene            complement(2712..2784)
                     /locus_tag="PKK76_14970"
     tRNA            complement(2712..2784)
                     /locus_tag="PKK76_14970"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2749..2751),aa:Ala,seq:ggc)
     gene            2836..>3511
                     /locus_tag="PKK76_14975"
     CDS             2836..>3511
                     /locus_tag="PKK76_14975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL69834.1"
                     /translation="MNGGPIRYIRGNVELVSYILIVVLVVLAGAGAVYGYQSLRGPRK
                     LEEIGNLIAGERYREALAECQRIIQQDDRSMIAHFLAGQCHQALSEYPMAVVEYKNCI
                     RIGKFDDQVKEIPVRRGLARSLIESGNANEAKNEFLILTTLEPNQFENHFELGRLFHR
                     GGIYPKSIKFLQTATALNVKNAEAFNVLGQSHYALKQYHDARAALIRAVQLKPDLKAA
                     HYYLGLA"
BASE COUNT          951 a          917 c          869 g          774 t
ORIGIN      
        1 tcttccagtc agactggagc cgtggcgggg acgcgatcat cgtggaccgc tccattcaca
       61 tgcctgagga gttgaaaggc aaacgcgtag catttcttcc cgcttcgccc tcgcacactc
      121 tgttgcttca tgtgctcgac accgcgaaac tgaactacag agaccttcag ctcgtggaaa
      181 caacctccgc acgtgatgcg gccacactct tcaagaatgg gaaagtagac gccgcagttg
      241 tatggagtcc agacgatgaa gattgcgaaa gcggcgccag gcgatgcgtc aaatccgttg
      301 caggctcacg catcctttcg agcaccacgc atgcaccaga catcatcgca gatgtattct
      361 tcgcaaaaaa atcttacatt gagaaacaca gagacgctct gcgatatttg attcagggct
      421 ggatgattgg aaacgcagaa atcaatgaat ctgtcgaggc cagatcccgt gcagcgcgta
      481 ttctttctgt tggaatgcgt cagcctgttg gattctgcgc ggaagcaatg aaaaaagtac
      541 gactggtcac ttacggagac aacaggatct ttttcggact ggacaaacgt tcaacaaacg
      601 tgaccgctga agatctgtac acaagaatgg cagcgcgata caaaagtctc cacctggcgc
      661 ctgagaacgc gccagcctgg aagcaaattg tagatacgtc tatactatcc actgtgaccg
      721 gactaaccgg aaatcagtcg cccgaaaatt agcgcagaac gcacgcgaaa aagaattgcg
      781 cggacgaatc ccggactcac cttccggcca tgggaaaacc agttgtacat tttgaaatcg
      841 catccgcaaa ttcaacggaa gctcagacat tctactcaaa gctctttggc tgggagatcg
      901 acgcaaacaa tccaatgaaa tatggaatcg tcaagaccgc aaaaggtgtc gggatcaatg
      961 gtggattatt ccaggcagac gcaggaaaag aagctgttgt agtctatgtc gcagtggaag
     1021 acacagatgc atacctcaag aaggttgttt ctatgggtgg gaccgtaatt catccaacag
     1081 aagtaattcc aggcatggtc accttcgcat tgtttgccga tccgtttgga acggtgatgg
     1141 gtcttatcaa agacgttcca ccgccaaaac ctgcgcccgc aatcgcggcc gggaagaaag
     1201 caaatcctgc tgcaaaaaca aaagctgtta agaaagccgc gcctaaaaag aaggcaaaga
     1261 aggcagcgaa aaagccagcg gcaaaaaaga agagaaaata aactgagagg gcgccggaga
     1321 ttctccggcg ctcaaactat gaccttggtt ctgtttgctt ctagaagatc cagctcatcg
     1381 ttatatccaa atttcttcgg atctttcaag cgatccacat aaagcacacc gtccaggtga
     1441 tcgcattcgt gctgatacac gactgcatca aacccttcta tcacctcatc atgaaactct
     1501 tcattttcat catagaattt cagacggatc ttgcgaactc tttccacgta cccgcgcata
     1561 tcaggaacgg aaagacaacc ttcccagaaa ccaacgggtg gcgcttttaa gatttcaatc
     1621 tcaggattca cgagaatacg ccgctcaatc ccctgcttca gctctggata tcgatcgctt
     1681 ctatcaaatc caacaatgac aatgcgcttg tggacaccca cctgcggtgc tgccagtcca
     1741 attccgccgg cttctgccat ggtatcaaac atatcacgaa tgagtttctt gatctccgcg
     1801 gagcgaatct ccgtgacagg aacaggttct gactttttgc gcagctccgg gtgaccaatt
     1861 cgtaagattt ttctgactgc catatccctt gttccgtggt tctaattaaa gcgaagactc
     1921 accggccgaa cagccctttg atgaaaccaa ctacggactc aaggccgcgt tgcaggtaac
     1981 cctcgggtcg attcagaaag tccatcaaca gagggttgat cgtggccgca ttctctttct
     2041 ggagaaagtg gcccatcccg gcaaagcgaa tcaagtggct gttcgtaaga ttttcgttta
     2101 tatcgcccac aggaggatca ggaacgcgcg gcatgcgatc tccggataag atcaaggttc
     2161 gggcctgcac tcgtgtgaga tcaggcgcag gtagtgctcg ccattcttct acccattgcc
     2221 gcatggcagg ggcggaaata gaagcgagtc tggccactgg ccgatgaaag gacggcccct
     2281 tttcgcctag agtgtctgcg aaaaggaaga atatttcata gaagaaaggc atgaatctgg
     2341 gcagaccccg catcaagttc agcggattga gcacgggcga aatcaagatg agttgcgcga
     2401 cccggtccgg gaagtcactg gccaggtcca ctgccagtct acaggagtgg ccgtgcgcga
     2461 tgatcgatat catttcgaca gagcccacaa tctgatccag gcgactgcga ttgtcctcaa
     2521 gcaaggaaga ggtggatgag ccctgtgcac cccggtggcc cgaaagggga aaattcagta
     2581 cgtcgtactg ccgcgaaagg aagtcgattt gctctgccca cgcgtccgta tccgaaaaca
     2641 cgtcatggat gagcaacagg ggtggtcttt ccccggagat tatttcttct gaatctggag
     2701 tcacttctac ctggagacga gggggatcga acccctgacc tttagaatgc cattctaacg
     2761 ctctcccaac tgagctacgc ccccgttcaa ggtcaggttt tgcgcgaaag ggaccgtagc
     2821 aatccctttt tgttcttgaa cggtggccct attcgttaca tacgggggaa cgtggaactt
     2881 gtcagttaca tcctgatcgt cgtactcgtg gtgctcgccg gagcaggcgc cgtatacgga
     2941 taccagagcc tccgagggcc tcgcaagcta gaagaaatcg ggaatctcat cgcaggggaa
     3001 cgctatcgcg aagctctagc agaatgccag cgaatcattc aacaggatga tcgctccatg
     3061 atcgctcatt ttctggcagg tcagtgccac caggccctca gtgagtatcc catggccgtt
     3121 gtagaataca agaattgtat tcgcattggc aagtttgatg atcaggtcaa agaaattcca
     3181 gtacgacgcg gcctcgcacg atccctgatt gaatccggaa acgcaaatga agcaaaaaat
     3241 gaattcttaa tcctcacaac tcttgaaccc aaccagtttg aaaatcattt tgaactgggc
     3301 agattgtttc atcgcggcgg catctatcct aaatccatta aattcttgca gacagcaacg
     3361 gcgcttaacg ttaaaaatgc agaagcattc aatgtgcttg gtcaatcgca ctatgcactc
     3421 aaacaatacc acgacgcccg ggccgcgctg atccgggcgg tacaactaaa accagatctc
     3481 aaagcggctc actactacct gggactggct c
//
LOCUS       DAORFF010000429         3503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_16928_length_3503_cov_3.778132, whole genome shotgun sequence.
ACCESSION   DAORFF010000429 DAORFF010000000
VERSION     DAORFF010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16928_length_3503_cov_3.778132"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PKK76_14980"
     CDS             complement(<1..651)
                     /locus_tag="PKK76_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HNL69835.1"
                     /translation="MKFWTRAGLILGLLCAIGVLANETANRKRHPLDLVYEKFAGTSI
                     KTADPDTVLRRMSLHLRGTIPGTKEVLAYEKADPETRDLSFAVDFLRSPDFAGYWGSY
                     FDSLFRGQTTAWKVPQAAFFKYIAESLHANKPYDVLVREMITSSGSPDKNPAVYFYVR
                     DGADPLQVAEYVGRVFDARRVGCARCHDHPFDTGFTRRDYYALGAFFSQQYIKWDSW"
     gene            complement(660..1994)
                     /locus_tag="PKK76_14985"
     CDS             complement(660..1994)
                     /locus_tag="PKK76_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNL69836.1"
                     /translation="MCADRFYPRGPAIVSGKREGMQSRKEFLQKSAALLGAFFLPGHL
                     MPEEDYEDITVPSSVKSVIFLNMAGGMSHVDTFDPKAGQSVFPGVATSISGIQFAETM
                     KATAKEASRVSLIRSTWSEDGDHSFGQKLLHTGYRVPQAQAFPDIPSFGAIIAYAKKR
                     KDKGPYFPSHITMGSRGGLTGRSGFLGVKYGAFQIGNLDNPVTHLKPAVGRIAEDRKG
                     RRGKMLDMLNESFSKDTSSAQVDQWKQMYLAALDFANSERLDVFDISKEKPELRAKFG
                     ESSIGKSLLMAKRLAQAEVPFIELTVGGWDTHNDNAAKIKGITKDLDPALAALLGELG
                     SSGLLKQTLVVLSSEFGRTPDVGTRDGRDHWPKVWTTLIAGGSIPQGAVIGESDPKGQ
                     KPAKRPVHVREIVATIYRASGVDPDAHPYNSMGRPFPLVPRGTEAVSELVGN"
     gene            complement(1984..>3503)
                     /locus_tag="PKK76_14990"
     CDS             complement(1984..>3503)
                     /locus_tag="PKK76_14990"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HNL69837.1"
                     /translation="FAHYWGVYFASVFREQTKGRKVKYASFLNYIAQSLHQNKPYDVW
                     TREMITATGSPEKNPAVDMYLRDGADPLQVAEYVGRVFYGERVSCARCHDHPHMKFSR
                     RDYYALAAFFSQQFEVRGSFDPTLFDGRAVPESLLENLPEQKRKEMEQKYRDWHRDAW
                     KNMSKEERAEYKKKFEVQYATLYFAQDLGVRFPKSDDQPGGDLVAPKFPDGTVPKIRE
                     GEDRRVVFATWLTAQPRFRKVLINRIWTRLMGWSFFAPLDDWNENTKIQNEAVLGHLD
                     KVFVEKNMRIKDLIYYIVTSKAYARRTSAPGDSDPSSIAYFQATRLDPDQLMNSILRA
                     SGSLDVKAGDLRERSIAGADRFSGRGELLKTEENAKEIQNAYQVPRPADDRSILAVFG
                     SGDRMDIADDDKSITVEQVLTLLNGRFTGRLSWDYGKAGSAVAATYDRTKDMNQVYDT
                     VFLSVLSRPAKAEEKERLRVLSAEKVGREKGEYRPEFLQDVVWAMFNSQEFLHVR"
BASE COUNT          843 a          935 c          807 g          918 t
ORIGIN      
        1 ccaggaatcc catttgatgt attgctgtga aaagaatgcg cccagagcgt aatagtcacg
       61 cctcgtaaat cctgtatcga acggatggtc atgacagcga gcacaaccta ctcttctggc
      121 atcaaacact cgacccacat attctgccac ttgcagcgga tcggcaccat cgcgcacata
      181 gaagtacact gctggatttt tatcaggcga tccgctcgac gtgatcattt cgcggactag
      241 aacatcatac ggtttattcg cgtgcagaga ttcagcaatg tatttgaaga aagccgcctg
      301 tggaactttc cacgcagtgg tctggccgcg aaatagactg tcaaaataag aaccccagta
      361 acctgcgaag tcaggagacc tgagaaaatc tacggcgaaa ctcagatctc ttgtttcagg
      421 atctgccttt tcgtatgcga ggacttcttt tgtgcctgga atggttccgc gaagatgcaa
      481 agacatcctg cgaaggacag tatccgggtc tgccgtcttg atggacgtgc ctgcgaattt
      541 ctcatacacc agatccagag gatgacgctt tcgattggcc gtttcgtttg caagtacacc
      601 gatggcgcac aataggccca gaatcaaacc agcgcgagtc cagaatttca tcttcattct
      661 caattcccca caagctcact gactgcctct gtcccgcgag gcacaagcgg aaaaggtcta
      721 cccatagaat tatacggatg cgcatccgga tccactcctg acgcgcgata gatggtggcc
      781 acgatttcgc gcacatggac cggtcttttt gccggctttt gtccttttgg atcactttcg
      841 cctatgactg caccctgagg aatgctgcca cctgcgatca gagtagtcca gacttttggc
      901 cagtggtctc ttccgtctct cgtcccaaca tctggtgtgc gaccaaactc gctagaaagg
      961 acgactaacg tttgtttgag caaaccagaa cttcccagtt ctcctagaag tgcagcaaga
     1021 gctggatcga gatccttcgt gattcccttg atcttggccg cattgtcatt gtgcgtatcc
     1081 catccaccca ctgtcagttc aatgaatgga acttccgctt gagcgagtct ctttgccatc
     1141 agaagtgatt ttccaatcga gctttccccg aacttcgcgc gaagctctgg cttctctttg
     1201 cttatgtcaa acacgtcaag gcgttctgaa ttggcaaaat caagagcagc gagatacatt
     1261 tgcttccact ggtctacctg agcagaagag gtatcttttg aaaacgattc gttgagcatg
     1321 tcgagcatct tgcctcgtct gccttttcta tcttccgcaa ttctgcctac agccggtttt
     1381 agatgagtga ctggattgtc caggtttcca atttgaaacg cgccgtactt aacgcctaaa
     1441 aagccggacc tgcccgtcag tccgcctcga ctgcccatgg ttatgtgcga aggaaagtaa
     1501 gggcctttgt cctttctctt tttcgcatac gcgatgatgg caccgaaaga tggaatgtct
     1561 ggaaacgcct gcgcctgcgg gactctgtat ccggtatgaa gaagtttctg tccaaagcta
     1621 tgatctccgt cttcagacca cgtacttcga atgagagaaa cacgcgacgc ctcttttgcc
     1681 gtggctttca tcgtttctgc aaattgaatt ccggaaatgg aagtggccac tccaggaaac
     1741 acagattgtc cggcctttgg atcaaacgta tctacgtggc tcattccacc tgccatgttt
     1801 aggaagatca cagatttgac ggaagaaggg acggtgatgt cttcatagtc ctcttcgggc
     1861 atcaaatgcc ctggcagaaa gaaagcaccc agaagggccg ccgatttctg caaaaattct
     1921 ttgcgagatt gcattccttc tctcttgcct gaaacaatcg cagggcctct gggataaaac
     1981 cgatcagcgc acatgaagga actcctggct attgaacatg gcccacacta catcttgtaa
     2041 aaattcaggt cggtattccc ccttctctct tcctactttt tcagcagaga gcacgcgaag
     2101 gcgctctttc tcttctgcct tcgcgggccg tgaaagcaca ctcaggaaga cagtatcata
     2161 tacctggttc atgtcctttg tgcgatcata ggtggcagcc acggcagaac ctgctttgcc
     2221 gtaatcccag gagagtctgc cggtgaatct gccattgagt aaagtcagca cctgctccac
     2281 tgtgatcgat ttgtcatcgt ctgcgatgtc cattctatct cctgaaccaa acactgcgag
     2341 aatagaacga tcgtctgcgg gacgaggaac ctggtatgcg ttctgtattt ctttcgcgtt
     2401 ctcctcagtc tttagcaact caccgcgccc cgaaaagcga tccgcacctg caatcgagcg
     2461 ttccctcagg tctcctgcct ttacgtcgag agaaccgctc gcgcgaagaa ttgaattcat
     2521 caattgatcc ggatcgagtc gcgtcgcctg aaagtaagca atcgaagaag gatcagaatc
     2581 tccaggagca gaagtgcgac gcgcatacgc tttcgacgta actatatagt aaatgagatc
     2641 tttgattcgc atgttctttt ctacgaagac tttgtccagg tgacccagca ctgcttcatt
     2701 ttgaatcttc gtgttttcat tccaatcgtc caggggtgca aagaagctcc atcccatgag
     2761 cctggtccag atacgattga tcagaacttt gcgaaaacga ggctgtgcgg taagccatgt
     2821 ggcaaataca actcgcctat cttctccttc gcgaatctta ggaaccgttc catctggaaa
     2881 tttgggcgcc accagatccc ctcctggctg gtcatcggat tttggaaatc tgactccaag
     2941 atcctgcgcg aaatagagag tagcatactg aacttcaaat ttctttttgt attccgctcg
     3001 ctcctctttg gacatattct tccaggcatc ccgatgccag tctcgatact tctgttccat
     3061 ttctttgcgc ttttgttccg gcaagttttc caggagagat tccggaactg cgcgaccatc
     3121 gaacagagtg ggatcaaaag aaccgcgcac ttcgaactgc tgcgaaaaga aagcggccag
     3181 tgcgtagtaa tctctgcgag aaaacttcat gtgcggatga tcatggcatc gcgcacagga
     3241 aaccctctct ccataaaaca cgcgtcccac atattccgct acctgcagag gatctgctcc
     3301 atcccgcaaa tacatgtcta cggcaggatt cttctctgga gagcctgtgg ccgtgatcat
     3361 ttcccgggtc catacatcgt atggtttgtt ttgatgaaga gactgagcta tgtaattcaa
     3421 gaagctcgcg tattttactt ttctcccttt cgtctgttcg cgaaacactg aggcaaagta
     3481 aactccccag tagtgcgcga aat
//
LOCUS       DAORFF010000430         3494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_17005_length_3494_cov_2.861006, whole genome shotgun sequence.
ACCESSION   DAORFF010000430 DAORFF010000000
VERSION     DAORFF010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17005_length_3494_cov_2.861006"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1133
                     /locus_tag="PKK76_14995"
     CDS             42..1133
                     /locus_tag="PKK76_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69838.1"
                     /translation="MLPIEASALRFLVKVLCATLFLSLMPGCSARMNSVPLSAQNPVA
                     FHLEHEGPLGIFDPSFAQSPDGIFWMSYSSVDFGWSDPRNGAVSTRLARSNDAVRWSV
                     MAGALNPSVSKGASRQTVHEVSNLVFDASAPENEKWKIFWHEYEWVAGERDFRHGWIS
                     MRTSANPEGPYSSRKKLFVGWGFLSDGQDEPLIRLDLLHADLGGCVAFTEPAAIYHDG
                     SLYLALTCAQILKSRGRIILLRESGSEWTYVGTILTNDADAAPTGYDGFSAPEFAQVG
                     PQLHLIVTPTIGDIYAGCAIYSIEDLRTASVRRLSGRATLSRFIAGPARFQGACGFRG
                     GSSPDLYFGEAHFDELPIFHLFRFPFPDL"
     gene            1275..2042
                     /locus_tag="PKK76_15000"
     CDS             1275..2042
                     /locus_tag="PKK76_15000"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNL69839.1"
                     /translation="MRTGECSIALCSWRRTVVGAIVFAIGSCTQGLPGHEKETRGDLA
                     LILVGLTPNLSGLVARYTFTGSANDSVSGRNLSVGAGAPTLTADRFGTPASAYSFNGA
                     SYFTASDSVPTALPSGSNPRTVCIWYNLTTLSGVTLTNYGTGVAGQAFGLGTNSLFSS
                     VFTYTYDLNIFLSPRTNVWTHYCGVYSGSDIKACINGALVAETTATSSGINTVLSSLA
                     VGKSNFQADFLTGSLDDLRIYNRALSAIDVAAIYSGW"
     gene            complement(2100..2729)
                     /locus_tag="PKK76_15005"
     CDS             complement(2100..2729)
                     /locus_tag="PKK76_15005"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNL69840.1"
                     /translation="MSAKKIILGVVIALVTIVVGGYFYLRSSLPRKEDFANLVEPRIS
                     TMQEITVLRVDFNGDADTVISDAYTRLFSAYYKLEGVPKGSSPAPLARYENLANVDFA
                     HTEDLKKIPWKGFAAIQIPDQTNLNDAARAAGATIEKLPYGEVAEIVHFGPYSTEHPT
                     ILKLHRFIESRGYKISGLHEEQYYKGPGMFIPVKPEDYITVVRYQISRK"
     gene            complement(2732..2890)
                     /locus_tag="PKK76_15010"
     CDS             complement(2732..2890)
                     /locus_tag="PKK76_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69841.1"
                     /translation="MQIGETLLSKGIINRDQLEKALDEQKKTGARLGDVVAQLGFATK
                     DQIEAALK"
     gene            3063..>3494
                     /locus_tag="PKK76_15015"
     CDS             3063..>3494
                     /locus_tag="PKK76_15015"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNL69842.1"
                     /translation="MDIKDLLPWGRDKWSLDASFGSLQKEMNRIFDDFTRGLDTRSRS
                     FLSHFDPKINVAEDAKSIQVTAELPGMDEKDVDVSVNDYALTIKGEKKWEQESKEQDH
                     HLVERSYGSFRRVIPLPQGVDTSSIKATFSKGVLKVEVAKSQ"
BASE COUNT          768 a          853 c          919 g          954 t
ORIGIN      
        1 cagagaaaca tggtcaaaga gactctggat aaggcgtcag aatgcttccg attgaagcaa
       61 gcgcattgag gtttctcgtg aaggttcttt gcgctacatt gtttctctcc ttgatgccag
      121 gatgcagcgc gcgcatgaac agcgttcctc tctcagcgca gaatcctgtg gcttttcatc
      181 tcgagcacga aggacctcta ggaattttcg atccgtcttt cgcacaatcg ccagatggca
      241 tcttctggat gtcttattcg tccgtggatt ttggatggtc agatccgcga aacggtgcag
      301 tctctacacg tcttgcaaga tcgaacgacg cagtacgatg gagcgtaatg gcaggcgcac
      361 tcaatccttc tgtcagcaag ggcgcatcca ggcaaacggt gcacgaagta tcgaacctgg
      421 tttttgacgc aagtgcgccg gaaaatgaga aatggaaaat cttctggcat gaatacgaat
      481 gggtcgcggg cgaacgcgat ttcaggcatg gttggatttc tatgcgaacc agcgcgaatc
      541 cggaaggccc ctattcttct agaaagaaac tctttgtggg ctggggattt ttgtcggatg
      601 gccaggatga gcccctgatt cgccttgatt tgctgcacgc agaccttggt ggatgcgttg
      661 ccttcacaga accagcggct atctatcacg atggatctct ctatctcgca cttacatgcg
      721 cacagattct gaaatccaga gggagaatca ttttgcttcg cgagtcggga agtgaatgga
      781 cgtacgttgg gactatattg acgaacgatg cggatgctgc gcccacaggc tacgacggct
      841 tttctgctcc agaattcgcg caggttgggc cccagctgca cttgattgta actcccacga
      901 taggcgacat ttatgccggc tgtgccattt attcgatcga ggatcttcgc acagcgtccg
      961 tccggagatt atccggccgt gcgaccttgt ccaggttcat tgctggcccc gcgcgatttc
     1021 aaggagcgtg cggctttcgc ggcggtagtt ctccagatct ctatttcggc gaagctcact
     1081 ttgatgagct tcctatcttt catctgttca ggttcccttt tccggatctc tgaatacttg
     1141 ctcgaaatct ataataaccc taaaccatta atttttttta gcaacgaggc cccggagatt
     1201 ctcgatttgc gtcgcctgtg ttcttcagtt gcacctgcca aaggccgatg gtcagaatca
     1261 gggctcctgg caggatgaga acaggcgaat gcagcatcgc gttatgcagt tggcgtcgca
     1321 ctgtcgtcgg tgccatcgta tttgcgattg gatcgtgcac gcagggctta ccggggcacg
     1381 agaaggagac tcggggagac ctcgctctta tcctggtcgg tttgactccg aatttatcag
     1441 ggcttgtggc gcgttatacc tttacaggaa gtgcaaatga ctctgtgtct ggtcgcaatc
     1501 tgagcgtcgg agcgggagcg cccactttga ccgcggatcg atttggaacc cctgcgagtg
     1561 cctattcttt taatggagca agctatttca ccgcgtctga ttccgttcca acagctcttc
     1621 cgtccggaag caatccgaga actgtttgca tctggtacaa tctcacgacc ctatctggcg
     1681 tgactttgac caattacggt acaggggtgg cggggcaggc ttttggcctc ggtactaact
     1741 ctctgttcag ctcagttttc acttacacgt acgatctgaa tatctttctg tcaccgcgaa
     1801 ccaatgtgtg gacgcactat tgtggagtgt actccggctc tgacatcaaa gcatgtatca
     1861 atggcgctct cgtcgcagaa actactgcga cgtcgtctgg aatcaacacg gttctctcca
     1921 gtctggcggt gggaaagtca aactttcagg cggattttct gactggctcc ctggacgatc
     1981 ttcgtattta caatcgagcg ttaagcgcca ttgatgtggc tgctatctac tctggctggt
     2041 gatcggtttc aaacgggccc ggtctgaagg aaggttcgca aagcctggtt tcagatggct
     2101 cacttgcgcg atatctgata acgaaccact gtgatatagt cttctggctt cacaggaata
     2161 aacattccgg gtcctttgta gtattgctct tcatgcagcc cggaaatctt gtatcccctg
     2221 gattcaataa aacgatgcaa ctttagaatg gttggatgct ccgtgctgta agggccaaaa
     2281 tgcacaattt ccgctacttc accgtaaggt aatttctcga tggttgcgcc cgcggcgcgc
     2341 gccgcatcgt tcaaattggt ttgatctgga atctggattg cggcaaaacc tttccatgga
     2401 atcttcttga gatcttccgt gtgtgcgaaa tccacattgg ccaggttctc ataccgtgcg
     2461 agaggggcag gcgaagatcc ttttggtacg ccttcgagtt tatagtacgc agagaagagc
     2521 ctggtgtatg catccgaaat gactgtgtct gcgtctccgt taaagtcaac gcggaggact
     2581 gtgatttctt gcattgtgga aatgcgcggt tccactagat ttgcaaagtc ttccttcctg
     2641 ggaaggctgg agcgcagata gaagtatccg ccgaccacaa tcgtaaccag cgcgatgact
     2701 actccgagga tgatcttttt cgcactcatg actatttcag tgcagcttcg atctggtctt
     2761 tcgtggcaaa accaagttgc gcaactacat ctcccagacg cgcacccgtt ttcttttgtt
     2821 catccagagc cttttctagc tggtctcggt tgatgatgcc tttgctgaga agtgtttcgc
     2881 cgatttgcat ggcgcacgtt gaatttactg gatgatttgt ccatccactt tgagccgatc
     2941 gtcgcgcgga gacttttttg gcatgtcttc aggcctgaca cgcgtcatgt ttcactattg
     3001 atttctgtca tacggactga gttcgttctc ttgtatcttc gcagtaggaa atggaggttc
     3061 ctatggatat taaagatttg ttaccatggg gaagggacaa gtggtccctg gatgcgtcgt
     3121 ttggttctct tcagaaggag atgaatcgga tctttgatga tttcacgcgc ggtctcgata
     3181 caagatctcg cagctttctt tcgcactttg acccaaagat caatgtcgcg gaagatgcga
     3241 aatccattca ggtaaccgca gagcttcccg gcatggacga aaaggatgtg gatgtttccg
     3301 taaatgacta cgcgctgact attaaaggcg aaaagaaatg ggaacaggag agcaaagaac
     3361 aggatcatca cctggtggaa cggtcgtatg ggagttttcg cagggtgatt ccactcccac
     3421 agggcgtgga tacttcctcg atcaaggcca cgttttctaa aggcgttctc aaggttgaag
     3481 ttgcgaagtc tcag
//
LOCUS       DAORFF010000431         3489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_17054_length_3489_cov_2.558824, whole genome shotgun sequence.
ACCESSION   DAORFF010000431 DAORFF010000000
VERSION     DAORFF010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17054_length_3489_cov_2.558824"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PKK76_15020"
     CDS             <1..354
                     /locus_tag="PKK76_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69843.1"
                     /translation="SSEHVTVMFGPFNIPGVTYQLPTKISTRGSLWVRQVEYTLTGFF
                     ALPSRVRLFLSYMAQDVHIQYGSRTNQNFPADLFLSAYFSQARFSEVILRQYDHPRGN
                     GRFRRVSAGAEFRIY"
     gene            332..1447
                     /locus_tag="PKK76_15025"
     CDS             332..1447
                     /locus_tag="PKK76_15025"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNL69844.1"
                     /translation="MLNSGSTEKGKARFRLVGEVKNTLIFPAHELHSSMADSMNPSIA
                     ILGLGAIGASIARALYLNRIPFTLLARDQGRLAELNRGFLFTTGRKRVEINSSSGVMK
                     ASLISSGETYDRIFLGMKSVHLPESVVAARKLLTPSGKLILLQNGIPLDLLPSASAAF
                     CMDGIVGYNVQIKDGSYYQSNAGHLILGEDGRMPDKDLKLALEPHLPVILTDNAKGFR
                     WNKLAINCVINGLGAISGEPLGPIFGNKHGRELAIQLLEETGSVIKAMGVHEEIVPGG
                     ISVFKFGRGGWPRIVQHSVLRALGLKYRQIKTSMLQDIENHRPTEVRSIHGAVLDAAR
                     KVNVAVPRMKAVVETIGYIEKGEMSPYPGLLSELAKA"
     gene            1674..2696
                     /locus_tag="PKK76_15030"
     CDS             1674..2696
                     /locus_tag="PKK76_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69845.1"
                     /translation="MKSELESQSVKGHLQGESVTTFGPAAPGTGAYSGAVLAPNGKIY
                     LVPGSATSYRVIDPVAGTVSTFGPAAPGGSAFRGGVLAPNGKIYLIPFNATSYRTIDT
                     ATDTVGSFGTAPGGAAFQGGVLAPNGRIYMAPFNSTTYSYIDPTNDTVSSFGPAAPGS
                     SAYHGAVLGPNGKAYLIPAANTTFRYIDTFTDTVFALGTAAPGGDAFHGGVLASTGRI
                     YLVPYLSTTYRYIDTSNDSVVTFGPAAPGSNAFDGGVIGPGGRTYLVPHDAGNFYYID
                     PGTGTVATYGPGGLPPGAFMGGIVTSTGRVYFAPYNEATYRYTDTGAAGSLCTSILLS
                     GYLNKY"
     gene            2808..>3489
                     /gene="fabG"
                     /locus_tag="PKK76_15035"
     CDS             2808..>3489
                     /gene="fabG"
                     /locus_tag="PKK76_15035"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNL69846.1"
                     /translation="MSINLTGKAAVITGSARGIGKAIALKLASLGADVVISDINAEQA
                     AATAKEIASATGKRAISHAGDISKLDQAEALIDACVKEFGHIDILVNNAGITKDTLLM
                     RMKPEQWDAVIAVNLTGTFNCTQPAFKQMMKQRSGAIVNISSIAKDGNMGQTNYSASK
                     AGVVGFTKAVALEGASRGIRSNAIAPGFIKTDMTDAIPEAIRSRMVKMIPLQRAGLPE
                     DIANGVAFL"
BASE COUNT          858 a          893 c          899 g          839 t
ORIGIN      
        1 tcatccgaac atgttacagt aatgttcggg cccttcaaca ttccgggcgt cacctaccag
       61 ctgcccacca aaatcagtac ccggggctcc ctgtgggtcc ggcaggttga atacacttta
      121 acgggttttt tcgcgcttcc atcccgcgtt cgcctttttc tcagttacat ggcccaggac
      181 gttcacattc agtacggcag tcggaccaat cagaatttcc ctgccgattt gttcctgagc
      241 gcgtactttt cgcaggctcg gttcagcgaa gtcatcttaa ggcagtacga tcacccgcgc
      301 ggcaatggac gctttcgccg cgtgagcgcg ggtgctgaat tcaggatcta ctgagaaggg
      361 gaaggcacgc tttcgcctcg tgggagaagt gaaaaatact ttgatttttc cagcacatga
      421 gctacacagt tctatggccg attccatgaa tccttccatt gcaattcttg gtctcggagc
      481 catcggtgca agcatcgcac gcgccctcta cctaaaccgg attccgttta cattgctggc
      541 gcgtgatcaa ggcaggcttg cggagttgaa tcgcggcttt ttattcacaa caggtcgaaa
      601 acgtgtggaa ataaattcgt cttccggtgt gatgaaagcg tcactgattt catccggaga
      661 aacttatgat cgcatcttcc ttggaatgaa gagtgttcat ctgccggagt cagtggtcgc
      721 agcgcgaaag ctcctgaccc catccggaaa actgatcctg ctgcaaaatg ggattccact
      781 ggatcttctt ccttctgctt ctgctgcctt ctgcatggat ggaattgtgg gatacaatgt
      841 gcaaatcaaa gatggttcct attaccagtc gaatgcgggg catttgattc tgggcgaaga
      901 cggaaggatg cctgacaaag atttgaaatt ggctcttgag ccccatttgc cggtgatttt
      961 gacagacaat gcgaaaggct ttcgctggaa caaactcgcc atcaactgcg tgatcaatgg
     1021 tctgggagcc atcagcgggg aaccactcgg ccctattttt ggaaacaaac acggaagaga
     1081 gctggccatt caacttctag aagaaacggg cagcgtgatc aaagcgatgg gagttcatga
     1141 agagattgtg cccggtggga tttctgtttt caaatttggc agaggtggtt ggccgcgcat
     1201 cgttcagcat agcgtgcttc gagctctcgg actcaagtac agacagatca agacatccat
     1261 gctgcaggac atagaaaacc acagaccaac tgaagtgcga agcattcacg gtgctgttct
     1321 ggatgcagcc agaaaagtga atgtagcagt tccgcgcatg aaggcagttg tagaaacaat
     1381 cggttacatt gaaaagggcg agatgtctcc ttatcctggt ttgctttctg aactggcaaa
     1441 ggcctgaaag tttcgcatga aagaaaggat taccgttgca cagcatctta cgtaaaacta
     1501 gacttcgctc gttttcattt ttgatcgggt tctttttcct ttcggcctgc gctcgatttc
     1561 ctgtagattt gaagcagtac gatgctctaa ctgcattggt agtctattat aatgtcacac
     1621 gcgcctgttc gttttcgttt caatccgtaa cgcagcctgc agtctggcag tttgtgaaaa
     1681 gcgaattgga atcgcagtct gtgaaaggtc atttgcaggg ggagtccgtg actacatttg
     1741 gtccggctgc tcccggaacc ggtgcataca gcggagcggt ccttgcgccc aacgggaaga
     1801 tttacctggt tccaggttca gccaccagct acagagtgat tgatcctgtg gccggaacag
     1861 tgagcacatt tgggcctgca gcacctggag gctctgcgtt tcgcggcgga gtgctcgcgc
     1921 ccaacggaaa gatctatttg attcctttca atgctacgtc gtatcgcacc atcgatactg
     1981 ccacggatac tgtagggagt tttggaacag cgccaggagg agctgccttc cagggcggag
     2041 ttcttgcgcc gaacggtcgc atctacatgg ctccattcaa cagcactacg tattcctaca
     2101 tagacccgac caatgacaca gtatcctcgt ttggccccgc agcccctggt tcgagcgctt
     2161 accacggcgc agttctggga ccaaacggaa aggcgtatct gattcccgcg gccaacacaa
     2221 cgtttcgcta catagataca ttcactgata ccgtatttgc gttgggtacg gcggcacctg
     2281 gaggcgacgc atttcacgga ggagttcttg catctaccgg tcgcatttat cttgttccgt
     2341 acctaagtac tacgtatcga tacattgaca cctcaaacga ttcggttgtg acgtttggtc
     2401 cggcagcacc cggaagcaat gcttttgatg gaggagtcat tggcccgggc ggacgaacct
     2461 acctcgtacc tcatgatgcc ggtaattttt actatataga tcctggaaca ggaaccgtag
     2521 caacgtacgg cccgggaggt cttcctccag gtgcctttat gggcgggatc gttacttcca
     2581 caggaagagt ctacttcgcg ccttacaacg aggctactta tagatacacg gacaccggcg
     2641 ccgccggatc cctctgtacg tcgattctac tgagcggtta tctgaacaaa tactgatcgc
     2701 ctgtttgcct ggcgttcctt tcgcccaaaa acgctggtca tgagcaagtt cttcagagaa
     2761 ttggaccaag acggcacaat gccgtcgcaa caatcgggga gtcttccatg agcatcaatc
     2821 ttaccggcaa agcagccgtc attaccggtt ctgctagagg aatcggcaaa gcaatcgcac
     2881 tgaaactggc cagcctgggc gcagacgtag tcatcagcga tatcaatgca gagcaggcag
     2941 cagccacagc aaaagaaatc gcatcggcca ctggaaaaag agccatctca cacgcaggag
     3001 atatttcaaa actcgatcag gcagaagcac tcattgacgc atgcgtcaag gaattcggcc
     3061 atattgatat tctggtgaac aatgcaggga tcacaaaaga tacactgctc atgcgcatga
     3121 agccagagca atgggatgct gtgattgcag tgaacctgac tggaactttc aactgcaccc
     3181 agcctgcttt caagcagatg atgaagcagc gttccggtgc aatcgtgaac atttcttcta
     3241 ttgcaaaaga cggcaacatg ggccagacca actactccgc ctccaaggca ggtgtggtag
     3301 gatttacaaa ggcagtggca ctcgaaggcg caagccgcgg catcagaagc aacgcaattg
     3361 cacccggttt catcaaaacg gacatgacag acgcaattcc ggaagcgatt cgcagccgta
     3421 tggtaaagat gattccactt cagagagctg gactcccaga agacatcgca aacggagtcg
     3481 catttctat
//
LOCUS       DAORFF010000432         3471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_17196_length_3471_cov_3.163642, whole genome shotgun sequence.
ACCESSION   DAORFF010000432 DAORFF010000000
VERSION     DAORFF010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17196_length_3471_cov_3.163642"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="PKK76_15040"
     CDS             complement(<1..1340)
                     /locus_tag="PKK76_15040"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNL69847.1"
                     /translation="MKRYLIVSVLFLAACKNTHDPLFGLGDPGYFLLGPNRLSVYFND
                     PGVDEQTMHDKKVDMRLVDLINDAKKTVDIAVYNLGRQTIIDALILAHERGVKVRMVG
                     DVDEGATDGYRQIEFYRQIPFSLGNATGIQHNKFAVVDSKYVFMGTGNYTDSDLMRNN
                     NNFMVMESDTLAREYTREFEQMFYGKFAGAKLAPTHPQRQHYVNLTPIELYFSPYDGD
                     EAIKRMVQLIDGAKVQIQFMIFAYTHDEISAAMIRAARRGVLVRGVHDLTFIRGTSEE
                     APRLYSAGVWMSTGPFNREDGNENTSVIGVAAHGGKLHAKTLIIDGNIVCTGSFNWST
                     NAVENNDENLLVVQSPYVAAELQQQWEAVWAVSHPITNQLSHPSGEPASPGDVVISEV
                     MWAGSYDSSGVYTNDDDWIELRNMTNHSIDLSHWSLAWDTKDTYNYPIPDEFNW"
     gene            complement(1387..3240)
                     /locus_tag="PKK76_15045"
     CDS             complement(1387..3240)
                     /locus_tag="PKK76_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69848.1"
                     /translation="MKYFVLITFALLCAHGVAAQEKQPDQQKQVEQPAPPKNNKDAKD
                     LEQIKQTDVKMPSGTLTMQPSKPEPVKPTEKWLYEMNLFSLDLNEKKALLDRIQWKAS
                     FFTSFNYFNNSDLRVLNSTNQTSIEDTDDKLYFMLSGAEIDTFFPIHPRLDFRVGLFK
                     AGFWGHDQLGGRDANNDIKFTRSGANTVNFDYLYFDVHFLKEPTSTRRADVRVGRQGY
                     SIGGEIERDFYLDDTLDAIVFKADSKFGSLNLLLIDVYANGSNTSDVYSVQYLSFDDE
                     KVDGFDGDVNTYRYGFVYMFPFIRAQNSLKETHIEARAFYYFARFGGLNDGGSDRTNK
                     GTTGNFSDNDFSTMKGVRLNAGFRGYVRGQITYAESAGIDRKRPTVAFTSQDVDTNGQ
                     SWMLDVEVSLFKRIVLLYPSVYWSEGGKYFTDGRPYSHGFVGFKGDHAGGILSDLNWG
                     MYPSAYVDDDGIDDTPFERQRKSGTFIRHLGAAIGKPDVIYFRVDWWRMEDTNRVGLV
                     NGATPGISSILSTKYVGPDLYEQQIRVEILKYIYPTAATQLLAYRRFSTPMAEEMNFT
                     VDWNVLPNWRAWAVVGVLRPMRYYATAGLEQGVPQGTARFTGMQVGTSFYF"
     gene            complement(3250..>3471)
                     /locus_tag="PKK76_15050"
     CDS             complement(3250..>3471)
                     /locus_tag="PKK76_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69849.1"
                     /translation="TLVDYVQFTTAACSTSQTGLSSAVSAGIWTSGDCVAGNDYIRRL
                     PNATETNRSTDWAGNTIPPGIPTPGAANN"
BASE COUNT          837 a          898 c          829 g          907 t
ORIGIN      
        1 aaccagttaa attcatcggg aattggatag ttgtatgtat cctttgtatc ccaggcaaga
       61 gaccagtggg aaagatcaat cgaatgattg gtcatgttgc gaagctctat ccagtcatca
      121 tcgtttgtgt atacgccgct tgagtcgtaa gaacccgccc acattacctc tgaaatcaca
      181 acatctccag gggaagcagg ctcaccgctt ggatgagaaa gttgatttgt aattggatga
      241 gaaacagccc agactgcttc ccattgctgc tgaagttctg ctgcaacgta tggagactgc
      301 actaccagga ggttttcgtc gttgttttcc acggcatttg tggaccagtt gaatgatcct
      361 gtgcagacga tattcccgtc aatgatcagg gttttggcgt gaagcttgcc gccgtgcgct
      421 gccacaccaa tgacggacgt gttctcattt ccatcttcgc ggttgaaggg cccggtggac
      481 atccacactc ccgcagaata gagtcggggc gcttcctctg aagttccgcg aatgaatgtg
      541 aggtcatgaa cgccgcgcac cagaactccg cgcctcgccg ctcggatcat ggcggcggaa
      601 atctcatcgt gagtgtatgc gaagatcatg aattgaatct gcactttcgc gccgtcgatc
      661 agctgcacca tgcgtttgat tgcttcatct ccatcataag gagagaaata gagttcgata
      721 ggagtgaggt tcacgtaatg ctgtctttgc gggtgtgtgg gcgcgagttt tgcccccgcg
      781 aacttgccat agaacatttg ttcgaattcg cgcgtatact cgcgcgccag agtatcgctt
      841 tccatcacca tgaagttgtt gttgtttcgc attaaatcgg agtcagtgta gtttcccgtt
      901 cccatgaaaa catacttgct gtccacgacc gcgaacttgt tgtgctgaat gcccgttgcg
      961 tttccaagag aaaaggggat ctgcctgtaa aactcaattt gtctgtaacc atctgtggcg
     1021 ccttcgtcta cgtctcccac catgcgaact ttcactccgc gctcatgtgc tagaatgagt
     1081 gcatcaatga ttgtctggcg gcccagattg tacacggcta tatcaacggt ttttttggcg
     1141 tcgttgatca aatccacgag acgcatgtcg actttcttgt cgtgcatggt ctgctcatcc
     1201 actccgggat cgttgaaata tacagaaagt ctattggggc caagtaaaaa gtatccggga
     1261 tcccccaggc caaagagcgg atcgtgcgtg tttttgcacg cagcaagaaa taaaacagaa
     1321 acgatcagat accgtttcat tcgcacgccc cctgtctcat tcagacatcc ccgtcgccat
     1381 tgccaatcaa aaatagaagc tcgtacccac ttgcatccct gtaaaacgag ccgttccctg
     1441 aggaacgccc tgctcgagac ccgccgtcgc gtagtacctc atggggcgaa gaacgcccac
     1501 aacagcccag gcacgccagt ttggcagaac attccagtcg acagtaaaat tcatttcctc
     1561 ggccatgggc gtgctaaatc gcctgtaggc cagaagctgc gtggctgccg taggatagat
     1621 gtatttcaaa atctccacgc gaatctgttg ttcatacaaa tccgggccaa cgtatttagt
     1681 tgagagaatc gaactaattc caggagtagc cccgttgaca agccccactc gattggtgtc
     1741 ttccattctc caccagtcta cgcgaaagta aattacgtct ggcttcccaa tcgcagctcc
     1801 caggtgacga atgaaagttc cggatttgcg ctgtctttca aaaggtgtgt catcaatgcc
     1861 atcgtcgtct acgtaagcgg agggatacat accccagttc aggtcgctca gaatgcctcc
     1921 tgcgtgatct cccttgaatc caacgaagcc gtgcgaatag ggcctgccgt ccgtgaaata
     1981 ctttccacct tctgaccagt agacggaagg ataaaggaga acgattcgct tgaagagcga
     2041 aacctccaca tccagcatcc aggactgtcc gttcgtgtcc acgtcctgac tcgtgaaggc
     2101 aactgttgga cgcttgcgat caatccccgc actctctgcg tatgtgatct gtcctcgaac
     2161 gtagccgcga aaccctgcgt tgagtctcac tcctttcatt gtggagaaat cattgtctga
     2221 gaaatttcct gtagttccct tgttggttct atctgaaccg ccatcgttga gtccgccaaa
     2281 ccgggcgaag taataaaaag cgcgcgcttc tatgtgcgtt tccttgagag aattctgagc
     2341 gcgaatgaat ggaaacatat acacgaatcc ataacgatag gtgttcacat ctccatcaaa
     2401 cccgtccacc ttctcatcat cgaaactcaa atactgaaca gaatacacgt ccgatgtgtt
     2461 cgatccattc gcatatacat cgatcaagag aagattgaga gatccgaatt tactgtccgc
     2521 tttaaagacg attgcgtcca gagtatcatc gagatagaag tcgcgctcaa tttctccgcc
     2581 tatcgagtaa ccctgacgcc cgacccgcac gtccgctctt cgcgtggatg taggctcttt
     2641 gagaaaatga acgtcaaaat agagataatc aaaattgaca gtgtttgcgc cactccgggt
     2701 gaacttgatg tcattatttg catcgcgtcc gcctagctgg tcatgccccc agaagcctgc
     2761 cttaaataag cccactctaa aatctagcct ggggtgaatg ggaaagaacg tatcaatttc
     2821 agcacccgaa agcatgaaat agagtttatc gtccgtatct tcaatgctgg tctgatttgt
     2881 ggaattaagc acccgcaagt ctgaattatt aaagtaatta aaggaagtaa agaagctggc
     2941 tttccactga attcgatcca gcagcgcctt tttctcattt aaatcgagcg aaaacaggtt
     3001 catctcatag agccacttct ctgttggttt taccggctcc ggtttggacg gctgcatcgt
     3061 cagtgtcccg gatggcatct tcacatccgt ttgtttgatc tgttcgagat ccttcgcatc
     3121 cttattgttc ttcggtggcg ccggctgctc gacttgcttc tgttgatctg gttgcttttc
     3181 ctgcgcggca accccgtgag cgcataacaa tgcaaaggtg attaaaacaa aatacttcaa
     3241 atcattttcc tagttgttag ccgctccggg cgtcggaatt ccgggtggaa tcgtgtttcc
     3301 cgcccagtct gtagatcgat tggtctctgt tgcattgggg agtctgcgta tgtagtcgtt
     3361 gcctgccaca caatcccccg atgtccagat tccagcagag actgcactgg agagtccggt
     3421 ctgtgacgtg ctgcaggcag ctgtagtgaa ttgaacgtag tccacgagag t
//
LOCUS       DAORFF010000433         3470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_17201_length_3470_cov_4.098975, whole genome shotgun sequence.
ACCESSION   DAORFF010000433 DAORFF010000000
VERSION     DAORFF010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17201_length_3470_cov_4.098975"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKK76_15055"
     CDS             complement(<1..222)
                     /locus_tag="PKK76_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNL69850.1"
                     /translation="MTLPGGKPQRQIVETFFATAARGTEECVEREIRDLAARYAESKI
                     KDIRRTRAGVEFKGTLQAGYLACLWLRTAS"
     gene            319..2316
                     /locus_tag="PKK76_15060"
     CDS             319..2316
                     /locus_tag="PKK76_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69851.1"
                     /translation="MTWITDANNRIHLHVKVMLAVVYGLTLYPMLEIGFTMQDDALLA
                     LAYQSKSLSPEPISYLIHSAVKESQYFGRVWVIYGSLMSYLPFAFHSAVYYKIVAFTS
                     LTLNVVLFFFVLQRLFRTLHFALLAVVLFLAFLQNSLHYNLITSFVAHFMTGISFLFA
                     SVLLFDRYLNTKKTWHHAGAGLCLFMSFFTYELFVLYYCLIPVLVLSSVMERHSLTRF
                     KEALPHWREIVRELVPTAAVLLVYLIIYAGYRLAYPTKYTGIEIAFSPARYIESVYRL
                     TIGALPISLYYRHSYDSVFAYLSENLQGHRSSIFYVLVRAHADWLARAVLLAFLALVV
                     LRDRPKRMSGRNLLWLCGFCLFLFWIPGTLPSLTSRYQIFVQMGLPMYLVTSFSYYAF
                     IVALTAVLIFVNERINHSALRRALHAIVVVIVFSWSILTDYTNFYVAKLQSQNQRQWL
                     IMDDFMHTPEFAAVPAGSIVYAPTLWQHSPLAPMVEVKEYWQDYIRAMTLKKLSIIKT
                     ESEFKRAFQAGELAGVDVYYLKFSQERKDPNQFLVFAKVNSSGVQNGRPFFRADHAAV
                     FMRSAYREYTAYVPLSGIEKTERVIVDGEERMLRGSFVAAPLDKSSVKGDLIKTEFRA
                     PAIDIERISVSNYVEQYEFPYNEKPNVEYDLEQERRKLLHQ"
     gene            complement(2317..2733)
                     /locus_tag="PKK76_15065"
     CDS             complement(2317..2733)
                     /locus_tag="PKK76_15065"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNL69852.1"
                     /translation="MNEEVHQRGLISHWLHLFMRAQFLRFLIVGGINTAFAYGVFSLG
                     LFIGLHYAVASLTSIILGMFFSFFTQGKLVFKSNKPEELLRFLGVATVMYLVHTGLLK
                     VASTFEIDLYLAGAVLTVPLALLSYVLNKHLVFRRN"
     gene            complement(2730..>3470)
                     /locus_tag="PKK76_15070"
     CDS             complement(2730..>3470)
                     /locus_tag="PKK76_15070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNL69853.1"
                     /translation="TFKDFELIILDNRSTDNTLKIVRKFKDKRIRVVAHEKNLGAEAN
                     WNAALDQAKGKYIKILCADDYIYPDCLEKQVEALRAESSAVLVCAARHIVDEKGKRIM
                     KRGPRKSETVPGIEAVRRAIRGGTNIYGETSSALFRRDAQKKAGAFDASIPYVIDLDM
                     WTRLLLHGNLILMDEAVSAYRVGAASWSAEIMTKQKENFMRFIERIEANPAFCLTGTD
                     AIIGRFRATLQAALRQLFYKFVLGWRKS"
BASE COUNT          784 a          855 c          858 g          973 t
ORIGIN      
        1 ggacgcagtt cgcagccaca ggcatgcaag ataccctgcc tgaagtgtgc ctttgaattc
       61 tactccagct ctggtgcggc gaatgtcttt gattttagat tccgcataac gggccgcgag
      121 gtctctgatt tcgcgctcca cacattcttc tgttcccctg gccgcagtcg cgaagaatgt
      181 ttccactatt tggcgctggg gcttgccgcc gggcagggtc atggatagtg aatcgcgcct
      241 tgaagcgcgt cccaggcaac aaaaatttga ttgcgtgcgg cacctatcgt gcctgcctga
      301 gtttggagtc cctgctgcat gacctggatt acggatgcaa acaatcggat acatcttcac
      361 gtcaaagtga tgctggcagt cgtttacggc ctgactctct atccaatgtt agaaatcgga
      421 tttacgatgc aggatgacgc ccttctggct cttgcgtatc agtccaagtc gctttccccc
      481 gagcctatct cctatctcat tcattcggcc gtaaaggagt ctcagtattt cgggcgtgtc
      541 tgggtcatct acggctctct catgagctac ttgccgtttg cctttcatag cgcagtgtac
      601 tacaagatcg tagcgttcac atccctgacc ctcaacgtag ttttgttctt ctttgtgctc
      661 cagagacttt ttcgtacgtt gcattttgcg ctgctcgcag tggtgctgtt tctcgcgttt
      721 ttgcagaata gcctgcatta caatctgatt acgtcctttg tggcgcactt catgaccggg
      781 atcagctttc tatttgcatc cgttttgttg tttgatcgct atctgaacac gaaaaagacc
      841 tggcatcatg ccggagcagg attgtgcctg ttcatgtctt tctttaccta tgaattgttt
      901 gtactttact actgtttgat tccggttctg gtcctgagta gcgtaatgga acggcattca
      961 ctgactcgtt ttaaggaagc gttgccgcac tggcgcgaga tcgttcggga actggtgccg
     1021 actgcagccg tattgctcgt atatttgatc atttatgctg gctaccggtt ggcctatcct
     1081 acgaaataca ctggcattga aattgctttt tcacctgctc gatacatcga aagcgtctac
     1141 aggttaacta ttggagcgtt gccgatttcc ctctactaca ggcactcgta cgattctgta
     1201 ttcgcatacc tttccgaaaa cctgcaggga catagaagca gcattttcta cgtgctggtt
     1261 cgtgcgcatg cagactggct cgcgcgcgct gtgttgcttg cgtttttagc tctcgtagtt
     1321 ctgcgcgatc gtcctaaaag aatgtcggga cggaatcttc tgtggttgtg cgggttctgt
     1381 ctgttcctgt tctggattcc aggaactctg ccttcgttga cttccaggta tcagatattt
     1441 gtgcaaatgg gtcttcccat gtatctggtc acttctttct cctattacgc gttcattgtg
     1501 gctctaactg cggttttgat attcgtgaac gaaaggatca atcactccgc tttgcgacgt
     1561 gcgcttcatg ccattgtggt cgtcatcgta ttctcgtgga gcatactgac ggactatacc
     1621 aacttctacg ttgcgaaatt gcagagtcag aatcagagac aatggctgat catggatgat
     1681 ttcatgcaca ctccggagtt tgcggcagtg ccggcaggta gtattgtcta tgctccgaca
     1741 ctctggcaac attctccgct ggcccctatg gtggaagtaa aagagtactg gcaggattat
     1801 atccgtgcca tgacactaaa gaagctttcc ataattaaaa ctgaaagtga attcaaaaga
     1861 gcgttccagg ccggcgaact tgccggggta gatgtttact acttaaagtt ttcccaggag
     1921 cgcaaagatc ccaatcagtt cctggttttc gcaaaagtga attcttctgg cgttcagaat
     1981 ggacgtccat tctttcgcgc ggatcatgct gcagtcttta tgagatctgc ataccgtgaa
     2041 tacactgcgt acgtaccgtt atctggcatt gagaaaacgg agcgagtgat tgtagatgga
     2101 gaggaacgga tgttacgcgg ttcgtttgtt gccgcgcccc tggataagtc atctgtaaaa
     2161 ggagacttga tcaaaaccga atttcgtgcc ccggccatcg acatagaaag gattagcgtt
     2221 tcgaattacg tggagcaata tgagtttccg tacaatgaaa agccaaatgt agagtacgac
     2281 cttgaacagg aaagacgcaa gttgttgcat cagtgatcag tttcttctga acaccaggtg
     2341 tttgttgagg acgtaagaca atagagcaag cggaacagtc aaaacagcgc ccgccaggta
     2401 gagatcgatt tcaaatgtag atgcaacctt caggagtccg gtatggacca ggtacatcac
     2461 tgtggccact ccgagaaacc tgaggagctc ctcgggtttg ttgcttttga agaccagttt
     2521 tccctgagtg aaaaaggaaa agaacatccc cagaatgatg gatgttagtg aagccacagc
     2581 atagtgcagg ccaatgaata accccagaga gaacacaccg taggcgaacg cggtattgat
     2641 tccgcccaca atgaggaagc gcaaaaattg tgcacgcatg aacaggtgca gccagtgcga
     2701 aatcaggccg cgctgatgca cttcttcgtt caagattttc tccatcccag aacgaatttg
     2761 tagaagagtt gtctgagtgc tgcctgaagg gttgcgcgaa agcggccaat gatcgcatcc
     2821 gttccggtca ggcagaaggc gggattcgct tcgattcgct cgatgaagcg catgaagttt
     2881 tctttttgct tggtcataat ttcagcgctc caggatgccg cccccactct gtaagcggag
     2941 actgcctcat ccatcagaat caaatttccg tgtagaagca gccgtgtcca catatcaagg
     3001 tcaatcacat acggaatgga cgcatcgaat gcccccgctt tcttctgtgc gtcgcggcga
     3061 aacagcgcag aactggtttc cccatagatg ttcgtaccgc ctctgatcgc gcgtcgaacc
     3121 gcttctatgc ccggcactgt ttcgcttttc cggggtccac gtttcatgat tcgctttcct
     3181 ttttcatcca cgatgtgacg ggcagcacag acaagtaccg cagacgactc ggcacgaagg
     3241 gcttctacct gcttttccag gcagtcgggg taaatataat catctgcaca aaggatctta
     3301 atgtatttcc ccttcgcctg atcgagagcg gcgttccagt ttgcttccgc gccaagattc
     3361 ttttcgtgcg ccacaacgcg aattctctta tctttgaatt tgcgcactat tttcagagta
     3421 ttgtccgtag accgattgtc cagaatgatg agttcgaagt ctttgaaggt
//
LOCUS       DAORFF010000434         3452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_17351_length_3452_cov_3.896379, whole genome shotgun sequence.
ACCESSION   DAORFF010000434 DAORFF010000000
VERSION     DAORFF010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17351_length_3452_cov_3.896379"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PKK76_15075"
     CDS             <1..250
                     /locus_tag="PKK76_15075"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL69854.1"
                     /translation="GMNSGRAIRGNVGSTIRKENTLIGDAVNVASRLEHACEPGRVMI
                     SAVLYERVRGKVTVSRPYLLKAKGKSEAIRVYYLEAIQ"
     gene            342..773
                     /locus_tag="PKK76_15080"
     CDS             342..773
                     /locus_tag="PKK76_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69855.1"
                     /translation="MRSIADRLTGCMKKNLLVSLALVSLTLSLPLFAQTPPPERKPSP
                     ECKAMHEKMMEGRKARDAKLEKMLATLKTATGQKKVDVMSDILTELLTERKEMQAHMQ
                     QMMEMRKNGNGCEMMGPGHPMMDHRSGPGQDRPAPDHSEHH"
     gene            complement(826..1953)
                     /locus_tag="PKK76_15085"
     CDS             complement(826..1953)
                     /locus_tag="PKK76_15085"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL69856.1"
                     /translation="MKLAFLLLSAAWLALVVAAAAAFYRFRSSLGKLRKSAEAIGRGD
                     FSISVLTDERGHLGDFARVFDGMRVDLAQAALAIREVAGDRDATQERYKIIYERTGDP
                     IFTILPNLQITAANPAAARLIGTQKGALKGKNFFDLVSTENQGSLAPVILMKEFAEFI
                     ENRGSMARRMVFRRLDGKETPEYDVRLEFLNVEGVDEIIARAVPVRTDALLHGLVKES
                     LSFEMKSDLLFVDTLAQRLSRNVPRYCAEEESFNVTLGVREMLINAIEHGNLGITHSE
                     KSAAMSQDTYLDLLQERARNPAYGSKVVWVEYELDAQKVRYRITDQGRGFDHKSFTKQ
                     QMPDVRRPHGRGILITSECFDSVTYNETGNSVELIRFFPTA"
     gene            complement(1966..>3452)
                     /locus_tag="PKK76_15090"
     CDS             complement(1966..>3452)
                     /locus_tag="PKK76_15090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL69857.1"
                     /translation="PIMQMSQAARRIRAHDYSTRITMRRKDEFANLKDAFNEMAAEVG
                     AYAQTLEQRVAERTSELEQARKETGRILATVSDALFLLTPEGTLRGNYSRAMEDIFPG
                     QRIAGANLMDLLRPVIAPQTLLSVQTYLKLMFDSSRPDVTLKGLNPLARIQFSDGSGI
                     WVSSNFQRIHSDGKVTELLGIFSDITPQIRLQEELGQEKRRSEIQTKLVLDLLRVAPK
                     LLRMFLSELEADLPEVKQSISDQNRLTVMRRLHTLKGNAASLGLDMFAEALHVAEEAA
                     EKSSSDPTPYSAAIATVEQIHKESSALSEKIRDAATIQVDENFIVQAIRGIFERRGHK
                     IDLVTTNFDAKLIASKHERMIRDALIQFARNTIAHGWESPQERESNGKQSEMIITLSP
                     RELNGHIEVTYSDDGKGLDVEKIRKRGISLGLLSETDPVDGARAVELIFHPGFSTRTS
                     SEISIDAGRGVGLDLVRAAVEEAGGHIRTASNNQRTEFTIELPV"
BASE COUNT          806 a          870 c          862 g          914 t
ORIGIN      
        1 tggtatgaat tccgggagag ccatccgcgg aaatgtgggc agcaccattc ggaaggaaaa
       61 cactttgatt ggagatgccg tgaatgtagc gagcagactg gaacatgcat gtgaaccggg
      121 gagagtcatg atttctgccg tactctatga aagagtgagg ggtaaagtca cagtttcccg
      181 accgtactta ctcaaagcga agggtaaatc agaggcaatc cgggtctact acctcgaagc
      241 cattcaatag cagaaaagtt tgcgctactt tcgctttaca tccaatggct tttgctgaca
      301 aaaatcatct tttagaagca agacgtgagg caggaatgta ggtgcgatcc attgctgatc
      361 gtctaactgg gtgtatgaaa aagaatctat tggtatctct ggccctggtt tctcttacac
      421 tttcgttgcc tttatttgcg cagactccac cccctgaaag aaagccgagt cctgaatgca
      481 aggcaatgca tgaaaagatg atggaaggtc gcaaggctcg ggatgcgaag cttgaaaaga
      541 tgctcgcaac tctgaagact gcaaccggtc aaaagaaagt agatgtaatg tcggacatac
      601 tcactgaact tctgactgag cgtaaagaaa tgcaagcgca tatgcaacag atgatggaaa
      661 tgcgcaagaa tggaaacgga tgtgagatga tgggccccgg tcatcctatg atggatcatc
      721 gttcgggtcc gggtcaagat cgcccggcac cagatcattc tgagcatcac tgattcctga
      781 tgttttgctt tgcccgcgga tggtccgtgg gcgaagcgag tctttctatg cagttggaaa
      841 aaagcgaatc aactcaacgg agtttccagt ttcattgtaa gtcacactat caaagcattc
      901 gcttgtaatt agaatcccgc gaccgtgcgg tctgcgcaca tcaggcatct gttgttttgt
      961 aaatgatttg tggtcaaagc cgcggccctg atccgtgatg cgatatctga ctttctgtgc
     1021 gtccagttcg tattccaccc agacgacctt actcccgtac gcaggattcc ttgcgcgttc
     1081 ttgaagtaga tcgaggtacg tgtcctgaga catggccgca ctcttttctg agtgcgtgat
     1141 tccaagattt ccatgttcaa ttgcattgat gagcatttcg cgcacaccca gtgtaacatt
     1201 gaagctttct tcttccgcgc aatagcgggg aacgttacgc gagagcctct gcgcaagagt
     1261 gtccacgaat agaagatcgc tcttcatctc aaaggagaga ctttctttca cgaggccgtg
     1321 cagaagcgcg tctgttctta cggggaccgc gcgcgcgatg atttcatcta ctccttctac
     1381 attcaagaat tccagacgca cgtcgtactc cggagtttct tttccgtcta aacgacgaaa
     1441 gaccatacga cgagccatcg aaccgcgatt ttctatgaac tccgcgaatt ctttcatgag
     1501 tatgaccggt gccagcgatc cctggttctc tgtgctcacc agatcaaaaa aattttttcc
     1561 tttcagtgcg cctttctgcg ttccgatgag ccgagctgct gccgggttcg ccgctgtaat
     1621 ttgaagattc gggagaatcg tgaaaatagg atcccccgtt ctttcgtaaa tgatcttgta
     1681 gcgttcctgt gttgcatcgc gatcgcccgc cacttctcga atggcaagag ccgcttgcgc
     1741 aagatccacg cgcattccgt caaacacacg ggcgaaatct ccgaggtgac ctctttcgtc
     1801 cgtgagtacc gaaatgctga aatcaccccg gccaatcgct tccgcagatt tgcgaagctt
     1861 gcccaggctg gaacgaaaac gatagaacgc agcagcggcg gccacaacaa gtgcaagcca
     1921 tgcagcggag agcagcagga aggccaactt catctgtgcg cagttttata ctggaagctc
     1981 gattgtaaac tccgtacgct gattgtttga tgcagttcgg atgtgccctc ccgcctcctc
     2041 gactgctgct cgaaccaggt ccaggccgac tcctcgccct gcatctattg aaatttcgga
     2101 gcttgtccgc gtggaaaacc cggggtgaaa aatcagctca acagcgcgag ctccatccac
     2161 gggatcggtc tctgacagga gccctagaga gattccgcgt tttctgattt tttcaacatc
     2221 tagccctttt ccgtcatcac tatatgtaac ttcaatatga ccgtttaatt ctctggggga
     2281 tagagtaatg atcatttcac tctgcttgcc attgctttcg cgttcctgcg ggctttccca
     2341 gccatgcgcg attgtattgc gggcgaactg gatgagcgca tctcgaatca ttctttcatg
     2401 ctttgaagca atgagcttcg cgtcaaaatt cgtagtgacc agatcaattt tatgccctct
     2461 gcgttcgaag attccgcgaa ttgcctggac aatgaagttt tcatccactt gaatcgtggc
     2521 tgcgtctcta atcttctccg acagagcgct cgattctttg tggatttgtt ccacggtcgc
     2581 gatggccgcg gagtacggag ttggatcact ggatgatttt tctgcggctt cttccgcgac
     2641 gtgcagtgct tccgcaaaca tgtcaagacc aagggacgct gcgttccctt tcaatgtgtg
     2701 caaccttctc ataaccgtta ggcgattctg atctgagatc gattgcttta cttcaggaag
     2761 atccgcttcc agttctgaca aaaacattct cagcaatttg ggagcgactc gcaacaaatc
     2821 gagaaccagt ttggtctgga tttcgctgcg tcttttctcc tgtcccagtt cttcctggag
     2881 gcgaatctgt ggggtgatgt ccgagaagat cccgagcagt tcagtgactt tcccatcact
     2941 gtgaatgcgc tggaagttcg aggacaccca tattccagat ccatcagaaa actgtattct
     3001 cgcgagtggg ttgagtcctt tgagtgttac atccggacgt gagctgtcaa acatcagttt
     3061 caagtacgtc tgcacgctga gaagagtttg cggcgcaatg acaggacgaa gaaggtccat
     3121 cagattcgca cctgcgatcc tttggcccgg gaaaatatct tccatggcgc gagagtagtt
     3181 tccgcgaagg gttccctccg gcgtaagaag gaagagagcg tctgatacag tcgcgagaat
     3241 tctcccggtt tcctttcgcg cttgctcaag ttctgatgtt ctttctgcca ctctctgttc
     3301 gagcgtctgg gcgtatgcgc caacttctgc cgccatctcg ttgaatgcat ctttgagatt
     3361 ggcgaattcg tcttttcttc gcatagtgat tcgggtagaa taatcatgtg cccgaattct
     3421 cctggcagcc tgactcatct gcatgattgg cc
//
LOCUS       DAORFF010000435         3449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_17383_length_3449_cov_3.159694, whole genome shotgun sequence.
ACCESSION   DAORFF010000435 DAORFF010000000
VERSION     DAORFF010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17383_length_3449_cov_3.159694"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1089)
                     /locus_tag="PKK76_15095"
     CDS             complement(28..1089)
                     /locus_tag="PKK76_15095"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     response regulator"
                     /protein_id="HNL69858.1"
                     /translation="MNLKMFRPDLARATILIVEDDPDQLALLEYYLALMRFRTAGVST
                     GADARVWLEQNQTDLILLDVNLPDMDGLVLLAEVREKNPDQAVMIMSAEKRAELIVEA
                     LFEGAEEFLQKPFSLIELSTRMHNILEMVAFRRNAESLVEKLSKEREILARYFSPEIV
                     ESILKQGPASQGGEYIHTTVLVYDLRNSTGLAEELGSERFAEIINMITVDLMDLIFSN
                     KGTITKLTGDGLIATFGTRESAEDVHNAVKCALAMREYYETILPVAMEQGVELAGFGI
                     GLATGRAFAGNVGSFRRMEYAIVGDVLSRAGRLEALTKSLGHPILVDARTQSFVPEVP
                     VMRRFLGRGRGKEAQLFGL"
     gene            complement(1086..2258)
                     /locus_tag="PKK76_15100"
     CDS             complement(1086..2258)
                     /locus_tag="PKK76_15100"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL69859.1"
                     /translation="MTTIQFYIFAHKEESNRKNPLRLAVFGARPFLRLVPAFDAIAIN
                     PRDTRALVIGADPEDVARTASFLGALSYQVEGVSDYDRAVQKVEEFSPHIVLLDAVFN
                     DLSGIDLLEQIKKARPDTGVILVSHAPRPEQILHAFFEGASGFLTKPLFITEVDLRIR
                     SVLKKIAYRKQMQETANHLEAEKQLLLRYFSGDVAEKLLSGEITADLKGTRADVSMFF
                     LSLRNAAAIAKTMSAGDFSHFLSQIFVDVLDLVYSHHGSVNKLTGAGILATFGLPVAS
                     PNDAKNCVDCARSLQEHFQLMNETGAWPQRIEFGIGIATGSVFAGNIGSYRKMEYTVI
                     GDPANLAARLDQLSRKLGIDILADGATVARSGVQGAERIAESSVRGKKHDVEIFRL"
     gene            2248..3213
                     /gene="corA"
                     /locus_tag="PKK76_15105"
     CDS             2248..3213
                     /gene="corA"
                     /locus_tag="PKK76_15105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNL69860.1"
                     /translation="MVVNCVLYSAGHPVQNIEINEIRSHLGQQDSFIWLGLYEPNEEL
                     MKEVQKAFGLHDLAVEDAHNAHQRPKIEAYGDSLFLVLHTAQKNQDKVEYGETHVFIG
                     YGYIVTVRHGASLPYTTVRERCEKTPEHLCRGPGFPLYAVMDFVVDNYLPIVEEFEDE
                     LVALEETIFKGNSHEDTTERIYDLKREIMGLRRATVPLLEVCNNLTRVTTPLLTAEVQ
                     PYFRDVHDHVVRINEAMETLREMLNTAFQVNLSLVTMKQNDVVKKLAGWAGLLGVPTM
                     MASFYGMNFKYMPELEWKYGYPALMVVMISACLFLYYRLKKSDWL"
     gene            3248..>3449
                     /locus_tag="PKK76_15110"
     CDS             3248..>3449
                     /locus_tag="PKK76_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69861.1"
                     /translation="MAWYWASLPLVAVLANIAAFLSSLQDRDSFSRGFRVLLVLLAGG
                     NLSLAAVLMSEPSANAPLAFHVF"
BASE COUNT          856 a          931 c          831 g          831 t
ORIGIN      
        1 cgggcaagac tttttctatg aatgccttca cagtccgaac aactgagctt ccttgcccct
       61 gcctcgcccc agaaaacgac gcatcaccgg aacctcgggc acgaaagact gagttcgcgc
      121 atctacgagg atcggatggc ccagagactt cgtcagagct tccaggcgac cggcgcgcga
      181 aagaacatct cccacaatcg cgtattccat tctacgaaag gacccaacat ttccggcaaa
      241 tgcgcggcca gtcgccagac caatgccgaa tccggccagc tctaccccct gctccatggc
      301 tacaggcaag atcgtttcat aatattcgcg cattgccaga gcgcacttca ccgcattgtg
      361 gacgtcttct gcggattcgc gcgtcccgaa cgttgcaatc aaaccatcgc cggtgagctt
      421 ggtgatggtg cccttgtttg aaaatatgag atccatcaaa tccacggtga tcatgttgat
      481 gatttccgcg aatctctcgg agccaagctc ctccgcgagg ccagtagaat tcctcagatc
      541 gtaaacgaga actgttgtgt gaatgtattc gccgccttgt gaagcgggcc cctgcttcaa
      601 aatcgattca acgatctcag gagagaagta tcgcgccaga atctctcttt ctttcgaaag
      661 tttctccacc aggctttccg cgttgcgacg aaaagcaacc atctccaaaa tgttatgcat
      721 acgggtggaa agctcaatga gggaaaacgg tttttgcaaa aactcctccg ccccttcgaa
      781 caatgcttcc acaatcaatt ccgctcgctt ctccgcagac atgatcatca cagcctgatc
      841 tggatttttt tcgcgcactt ccgccagcag aacaaggcca tccatgtccg gaagattcac
      901 gtccaaaagg atcaggtctg tttgattttg ctctaaccac acgcgcgcgt cagctccggt
      961 cgaaactcct gcagtcctga acctcatcaa agccagataa tactccagta gagcgagttg
     1021 atccggatcg tcttccacga tgagtatggt tgcccgggct agatccggcc gaaacatttt
     1081 cagattcata gacggaatat ttccacgtcg tgtttcttgc cacgcactga actttctgca
     1141 atcctctctg cgccctgcac tcccgaacga gcaaccgtcg cgccgtcagc cagaatatca
     1201 atcccgagct tacgactcag ctgatcgagt cgcgctgcca ggttcgcagg atctccaatc
     1261 acggtgtatt ccattttacg ataagagcca atgtttccgg caaagacaga tccggttgcg
     1321 attccaatcc cgaattcaat tctctgcggc caggctcctg tctcattcat caattgaaaa
     1381 tgctcctgca aagaccgagc gcaatcgacg cagttcttcg catcgtttgg ggatgcaaca
     1441 ggaagtccaa aagtcgccag tatccccgcg cccgtcagct tgtttacaga accatggtga
     1501 gaatagacca gatctagaac gtctacgaat atctgactga ggaaatgaga gaaatcgcct
     1561 gcactcattg tctttgcaat ggcagccgcg tttcgcaaac tgagaaaaaa catagaaacg
     1621 tctgcccggg ttcctttaag atccgcggta atttctccag aaagaagctt ttctgccaca
     1681 tccccggaga agtagcgcaa caggagctgc ttttctgcct cgagatgatt tgcagtttct
     1741 tgcatttgtt tacgataggc gatcttcttc agcacacttc gaatgcgaag gtccacctca
     1801 gtaataaaaa ggggcttggt caagaatccg gatgcacctt caaagaaggc atgcaatatc
     1861 tgttcgggtc ttggcgcatg cgaaacgagt attacgcctg tatccgggcg cgccttcttg
     1921 atttgttcga gcaaatcgat tccgctgaga tcgttaaaca ccgcatcgag aagtacgata
     1981 tgcggggaaa attcttctac cttttgcact gcccggtcgt agtccgaaac cccttccacc
     2041 tgataggaca atgcacccag aaaagaggca gtgcgggcaa catcctcggg atcagctcca
     2101 attaccaggg ccctcgtatc acgtggattg atcgcgattg cgtcaaatgc aggcacaagt
     2161 cgcaggaatg gccgcgcgcc gaaaaccgca agccgtaatg gatttttgcg attgctttct
     2221 tccttatgtg cgaagatata gaactgtatg gtcgtcaact gcgtgctcta ttctgccggt
     2281 cacccggttc agaacataga aatcaacgag atacgctcgc acctcgggca gcaggattca
     2341 ttcatctggc ttggccttta tgaaccaaac gaagagttga tgaaggaagt ccagaaggcg
     2401 tttggtctgc atgatcttgc agtggaagat gcccacaacg cgcaccagag gcctaaaatt
     2461 gaggcatatg gagacagtct gtttcttgtt ttacacactg ctcaaaaaaa ccaggacaaa
     2521 gtagaatacg gggaaactca cgtattcatt ggctatggct acattgtcac cgtccggcat
     2581 ggcgcatctc tcccttacac aacagtgcgc gaaagatgcg aaaagacccc ggagcatcta
     2641 tgtcggggac cgggcttccc tctgtacgct gtgatggact tcgtcgtgga caactacttg
     2701 cccatcgtgg aagaatttga agacgaactg gttgcgcttg aagagaccat ttttaaaggc
     2761 aattcgcacg aagatactac ggaacgaatc tatgacctga agcgggaaat catgggcctg
     2821 cgaagagcca ctgttccgct gctggaagtt tgcaataacc tgacgcgtgt aaccacacca
     2881 cttctgactg cagaagtcca accctacttc agagacgttc acgatcacgt ggtgcgaatc
     2941 aacgaagcca tggaaactct gcgagaaatg ctcaacactg cattccaggt gaacctgtcc
     3001 cttgtgacca tgaagcaaaa tgatgtggtc aaaaagctgg cgggatgggc cggtttgctg
     3061 ggcgtgccga ccatgatggc tagcttctac ggaatgaatt tcaaatacat gccggaactc
     3121 gaatggaagt atggctaccc ggcgctcatg gtggtcatga tcagtgcatg tcttttcctt
     3181 tattatcgcc tgaagaaatc agactggtta tgattcgcgg tcctcactgc catgaggaga
     3241 acgtctagtg gcctggtact gggcttcgct gcctcttgta gccgtactcg caaacatcgc
     3301 ggcgtttctt tcttctctgc aagaccgcga ttcattcagc cgagggtttc gcgtactcct
     3361 ggttttgctt gcgggcggga acttgagtct ggccgctgtt ttgatgagtg aaccttctgc
     3421 aaatgctcct ctggcgtttc acgtatttc
//
LOCUS       DAORFF010000436         3447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_17400_length_3447_cov_4.043337, whole genome shotgun sequence.
ACCESSION   DAORFF010000436 DAORFF010000000
VERSION     DAORFF010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17400_length_3447_cov_4.043337"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..943
                     /gene="atpB"
                     /locus_tag="PKK76_15115"
     CDS             2..943
                     /gene="atpB"
                     /locus_tag="PKK76_15115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002153283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNL69862.1"
                     /translation="MDAPIFEWNVCVPGNLKIGEYPLCGKKVYEGSEAFEKDPFRRYV
                     FEDKDGKYKWEGGLPMHITRRVMMMFIVVALMLVIFIAGARAIARNPLATKGRYKNSI
                     EAMVQFVRNDIAEPNMHHHSAGFQPYLLTAFFFILFSNMLGLFPPFGELLDMALHGVH
                     PHHGTTASAVTAVWPGITVTGDVAVTMVLAIMTTIQIWITGFRYQGIQFIWTSVPNGV
                     PIWLYPLLWPLEFVISPLSKGFALTIRLLANMTAGHVIIIVLLGFIMQFKNYWLGFVS
                     VPGAGMIYMLEILVAFLQAFVFTLLSALFIGASMHRH"
     gene            1027..1320
                     /locus_tag="PKK76_15120"
     CDS             1027..1320
                     /locus_tag="PKK76_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNL69863.1"
                     /translation="MIGLAFIGVGLGAGLAVLGIGVGIGRIGGSATEAISRQPEAVGS
                     IQLAMIIAAALIEGAGLFGLVIAFLMGNQINEKMLNTFAGPAESGKPAATQTK"
     gene            1329..1853
                     /gene="atpF"
                     /locus_tag="PKK76_15125"
     CDS             1329..1853
                     /gene="atpF"
                     /locus_tag="PKK76_15125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNL69864.1"
                     /translation="MSATLFIASEGGLSLLSVNPGLAIWTTLTFLLVLGVLHRFAWQP
                     IIQALDERAGKIQSDLDRAEAVRKEAEMKLEDYLQKLNALKEQGTEIIEEAHKDANRI
                     KEELLESAKNEAEAMRQRGVRDIELAKDKALEELHKEVVSLSVGIASQIIGRTLKAED
                     HKRFVEDAIKGLRS"
     gene            1875..2444
                     /gene="atpH"
                     /locus_tag="PKK76_15130"
     CDS             1875..2444
                     /gene="atpH"
                     /locus_tag="PKK76_15130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNL69865.1"
                     /translation="MIGFEKVAVVYAEALLAAAVDAGPVTELEEDIAQLNELFHKDPD
                     VWAFFKSPIIEVDGKLSLLSKTLKGKVSDTLYSFLCVLCVRRRFELFPDIVEQVKTLL
                     DHHLKRKRVQVFSAVALTPEHSAELQGALKKRLNMDVVLEVHEKPDLIGGLVVRTGDL
                     KEDSSIKDSLARMAKALTGKRILGEKYYA"
     gene            2437..>3447
                     /gene="atpA"
                     /locus_tag="PKK76_15135"
     CDS             2437..>3447
                     /gene="atpA"
                     /locus_tag="PKK76_15135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNL69866.1"
                     /translation="MLKIKTDEISSVLKKEIQSFQSRLDLSEVGTVLSVGDGIARVYG
                     LENVMANEMIEFEDGTLGLAFNLEEGSVGVVILGEYTQLREGHQVKRLGRVLEVPVGE
                     SMLGRMVDPMGRPVDDRSLPIHTTKTRPVEFLAPGIARRQPVKEPMQTGIKAIDAMIP
                     IGRGQRELIIGDRGTGKTAIAIDTIINQKGKDVVCVYVAIGQKASKVAAIKDKLTALG
                     CMDYTIIVVATASDPAPLQYLAPYSGASMAEYFMYEKGKATLVIYDDLTKQANAYRQM
                     SLLLRRPPGREAFPGDVFYLHSRLLERAAKLSDKYGGGSLTALPIIETQEGEVSAYIP
                     TNV"
BASE COUNT          848 a          866 c          881 g          852 t
ORIGIN      
        1 tatggatgcg cccattttcg aatggaacgt ttgcgtcccc ggaaatctta aaatcggcga
       61 atacccgctc tgcggaaaaa aagtctacga aggaagcgaa gcgtttgaga aagatccgtt
      121 tcgccgttat gtcttcgaag acaaagacgg aaaatacaaa tgggaaggcg gccttcccat
      181 gcacatcact cgtcgtgtga tgatgatgtt cattgtagtt gctctgatgc ttgtcatctt
      241 catcgcagga gccagggcca ttgcgcgcaa tcctcttgct acaaagggaa gatacaagaa
      301 ttcaattgag gcaatggttc agttcgttcg caatgatatc gcagagccaa acatgcatca
      361 tcactctgcg ggttttcagc cgtatctgct cactgcattc ttcttcattc ttttctcaaa
      421 catgctcggt ctgttccctc cgtttggaga gctgctggat atggctctgc acggagtgca
      481 tcctcatcac ggaacaactg cctctgctgt aaccgctgta tggccaggga tcaccgttac
      541 aggggacgtt gcagtcacca tggttcttgc catcatgacc acaatccaga tctggatcac
      601 tggcttccgt taccagggca ttcagttcat ctggacgtct gttcctaatg gcgttccaat
      661 ctggctttat ccgcttctgt ggcctcttga gttcgtgatt tcccctcttt ccaagggttt
      721 tgcactcacc attcgtcttc tggcaaacat gactgcgggt cacgttatca tcatcgtact
      781 tcttggcttc attatgcagt tcaagaacta ctggcttggg tttgtaagtg tgcctggcgc
      841 cggcatgatc tatatgcttg aaatactggt tgcgtttctt caagcatttg tctttacctt
      901 gctgtccgcg ttgtttatcg gtgcaagtat gcacaggcat tgaaatggat tctcttaatt
      961 tatttagaaa gagcaatcaa gagagcgaat caaagttgat tcgccagtga tcaggaggag
     1021 aaaactatga tcggtttagc ttttattgga gtcggacttg gcgctggcct tgctgtactg
     1081 ggcattggcg tgggcattgg ccgcatcggc ggttctgcaa ctgaggccat ctcccgtcag
     1141 ccagaagcgg ttggttccat ccaacttgct atgatcatcg cggcagcgct gattgaaggt
     1201 gcaggtctct tcggtcttgt gatcgctttc cttatgggaa accagatcaa cgaaaagatg
     1261 ctgaatacgt ttgctggccc ggctgaaagc ggcaagcctg cagctactca gaccaagtaa
     1321 taacgttcat gtctgcaact ctcttcattg catccgaggg cggtctttcg cttctctctg
     1381 taaatccggg tcttgcgatc tggactaccc tgactttcct cctggttctg ggtgtgctgc
     1441 accgctttgc gtggcagcct atcatccagg ccctggacga aagagcgggt aagatccagt
     1501 ctgacctgga ccgggccgaa gcggtccgca aagaagctga aatgaagctt gaggattact
     1561 tgcagaagct caatgctctt aaggagcagg ggactgaaat catcgaggaa gctcacaaag
     1621 acgcaaatcg catcaaggaa gaacttcttg agtctgcgaa aaacgaagcg gaggcaatgc
     1681 gccagcgcgg agtgcgtgac attgagcttg cgaaggataa agccctcgaa gagctgcata
     1741 aagaagtcgt ttccctttcc gtgggcattg cgtcccagat catcggccgg actttgaagg
     1801 ctgaggatca caaacggttt gttgaagatg ccatcaaagg gcttcggtcc tgaacctgat
     1861 tccaggatgc ttcgatgata ggatttgaaa aagtagcggt agtgtacgcg gaagctcttc
     1921 ttgcagctgc cgtggatgct ggccccgtaa ctgagctgga agaagacatc gcccagctca
     1981 atgagttgtt ccataaggat ccagatgtct gggccttttt caaatcgccc atcattgaag
     2041 tagacgggaa actcagtctt ctttccaaaa ctctcaaagg gaaagtttca gatacgctgt
     2101 attcttttct ctgcgtgctc tgcgtgcgca ggcgttttga actgttcccg gacattgtag
     2161 aacaggtgaa aactcttctc gatcatcacc tgaaacgcaa acgcgttcag gtattctctg
     2221 cagtcgctct cacaccggag catagcgcag aactccaggg agcgctcaag aagcgcctca
     2281 atatggatgt agtgctggaa gtgcatgaga agccggatct aatcggcggc cttgtagttc
     2341 gcacaggcga tctcaaggaa gattcaagta ttaaagacag tcttgcccgc atggcaaagg
     2401 ccctcacggg caaacgaatt ctcggggaaa aatattatgc ttaaaatcaa aactgatgaa
     2461 atctccagcg tactgaagaa ggaaatccag agctttcaat ctcgcctgga cctttccgaa
     2521 gtcggaaccg ttctctctgt aggggacggg attgcccggg tatacggcct tgaaaacgta
     2581 atggccaacg aaatgatcga gttcgaagac ggaacactcg gtcttgcatt caacctcgaa
     2641 gaaggttccg tgggtgtggt aatcctcggc gaatacacac agctgcgcga aggtcatcag
     2701 gtaaaaagac tcggccgcgt tctggaagtt ccagtcggag aatccatgct cggccgcatg
     2761 gtggacccaa tgggccgccc tgtagacgat cgttctcttc caattcacac aaccaagact
     2821 cgccctgtag aattcctggc acctggcatt gcacgcagac agcctgtaaa agagccaatg
     2881 caaactggaa tcaaggcgat cgacgcgatg attccaatcg gccgcgggca gcgcgaactg
     2941 atcattggag accgcggaac tggaaaaaca gccatcgcaa tcgacacaat catcaaccag
     3001 aaaggcaaag atgtagtttg cgtatacgtt gcgattggtc agaaagcttc taaagtagcg
     3061 gccatcaaag acaaactcac ggccctgggt tgcatggact acacgatcat cgttgtggcc
     3121 acagcttctg acccggctcc tctgcagtac ctggctccct actccggagc gagcatggca
     3181 gaatacttca tgtatgaaaa gggcaaagcc actctggtga tctacgatga ccttacaaaa
     3241 caggcaaacg cgtatcgcca gatgtctctg cttcttcgtc gtccaccagg ccgcgaagcg
     3301 ttccctggag acgtattcta tctccacagc cgtcttctcg agcgcgcagc taaactgagc
     3361 gacaagtacg gcggaggttc tctcacagct cttcctatca ttgaaactca ggaaggggaa
     3421 gtgagtgctt acattcctac caacgtg
//
LOCUS       DAORFF010000437         3440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_17451_length_3440_cov_3.501625, whole genome shotgun sequence.
ACCESSION   DAORFF010000437 DAORFF010000000
VERSION     DAORFF010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17451_length_3440_cov_3.501625"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="PKK76_15140"
     CDS             complement(<1..1119)
                     /locus_tag="PKK76_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69867.1"
                     /translation="MAKKTQKKPAKKIEKPAKKPAKAPEKKPLPVKKALPVPAKKPTA
                     VKPAPPTRPAAREKGDFVSTWIARGQTFESVEQLFHEAAIFLRKNFGFTSLNLFSGSG
                     SSPLLAVHHDGTSYVSENLDTTGHAPDAGTITKESKVFIIAENKPSSVSIQGSIASAS
                     VNAPGHQSGILVAASEDPAPPYLKSDLALLQSFAQFLGPVLSHFQLMAVETKNRDLSA
                     ELKETQKNARESQKTLTADSARKIEELTAEHESRLSAQKESLLAEAEEKTRALAEKLE
                     ARIEESSQTALRLREELQTAHRERDEAAATARKESQDRIAELELELDDLKSRAAEQKA
                     AAIQEIETAAAAEKADLRAKLDAAEREKQSALSRTSALE"
     gene            complement(1123..1944)
                     /locus_tag="PKK76_15145"
     CDS             complement(1123..1944)
                     /locus_tag="PKK76_15145"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNL69868.1"
                     /translation="MKNRVYLVTGATSGIGRALALRAAQAGDTVFAGVRELSRAPSSE
                     NIRPIQLDVTNPEDVRRASETILQQCGNIDVLVNNAGFGVYGAFEELSEEQIRAQMET
                     NYFGCLAMARAVLPSMRERRSGTIINVSSILGRITIPTGSAYTASKYAIEAFTESLRQ
                     EVKPFGIQVFAVEPGLIRTSFKQNMVKSHAVQKNDSPYAFLNRMIQKEYSGFSTSQER
                     AADRIYKLSQKKRPARRYRVGLDASFYYTLSRILPDSLLDNLVALGVRREFKKTS"
     gene            2070..2142
                     /locus_tag="PKK76_15150"
     tRNA            2070..2142
                     /locus_tag="PKK76_15150"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2103..2105,aa:Ala,seq:tgc)
     gene            2437..3387
                     /locus_tag="PKK76_15155"
     CDS             2437..3387
                     /locus_tag="PKK76_15155"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL69869.1"
                     /translation="MFAKFFELFMAAENLKDIRKLVKEFLWKSLNTDQMNYVAKEADP
                     RFDLGKVSGFGHSIVVTRQTAADVIVDYFATEDAFLHLFATLLFMEGKNASGGVIHFV
                     NPDPIIKKLRERQWIYDPSAGRFIKSQSQTRTADWGFLQEGSEYSLVFGSIDIVGSSA
                     LARTNVPVDVENTLVRMRAYLQDQIEARNGRIWYWHGDGGVMAFYGADAASRAVISSV
                     AILAYLPIFNLTQNELRPENDVSLRIGLHAGLAVYRSDTEKILSPDIRFAEEVEKHCA
                     ETNTIAITERTVNSLPPEVRRQFNPAGEHSANRIYVYRPV"
BASE COUNT          747 a          856 c          897 g          940 t
ORIGIN      
        1 ttccagagca gacgtgcgcg acagtgcaga ctgcttttct ctctctgctg catcaagttt
       61 cgcacggaga tctgcttttt ccgcggcggc tgcagtttcg atttcttgga tggccgctgc
      121 cttctgttct gcggctctag atttgagatc gtcgagttcg agctctagtt ccgcgattct
      181 gtcctgggat tccttgcgcg ctgtggcggc cgcttcgtct ctttctctgt gagctgtctg
      241 cagttcttct cgcagacgca gtgcggtctg gctgctttct tcgatgcggg cctcgagctt
      301 ttctgcgagt gctctagtct tttcctctgc ttctgctaaa agggattctt tttgggcgct
      361 caggcgcgat tcatgttctg ccgtgagctc ttcaattttt cgtgcggaat ctgcggtcag
      421 agtcttctgt gattcgcggg cattcttttg cgtttctttg agctctgcgg acagatcgcg
      481 gttcttcgtc tcaacggcca tgagctggaa atggctgagt acgggcccca gaaactgggc
      541 aaaagattgc agcagagcca gatcactttt gagatagggt ggtgccggat cttcggaagc
      601 ggccacaagg attccgctct ggtggcccgg tgcattcaca gaggcagaag caatggatcc
      661 ctgaatggaa acagaagaag gtttgttttc tgcaatgatg aatactttag attctttagt
      721 gatggtcccg gcatccggtg cgtgtcccgt tgtgtccagg ttttctgaga cgtagctggt
      781 tccatcgtga tggacggcca gaagcgggga ggatccagat ccagagaata gattgagaga
      841 agtgaatcca aagttctttc tcaggaagat agccgcttcg tgaaacagct gttctacgct
      901 ttcaaacgtt tgtccgcgcg cgatccacgt gcttacaaag tctccctttt ccctggccgc
      961 cggccgtgtg ggaggagcag gtttcacagc cgttggtttc tttgccggaa caggcaatgc
     1021 tttctttacc ggtaggggct ttttttcagg tgcctttgcc ggtttcttag ctggcttttc
     1081 gattttcttc gctggttttt tttgggtctt ttttgccata aatcaagatg tctttttgaa
     1141 ttcgcgcctg actccgagcg caacgagatt gtctagtagg gaatctggaa gaatgcgaga
     1201 cagtgtgtag tagaaagaag catccaggcc aactctgtat cttctggccg gtcttttttt
     1261 ctggctcagc ttgtagattc tatccgcggc tctttcctgt gacgtggaaa aaccgctgta
     1321 ttctttctgg atcatgcggt tcaaaaatgc gtaaggcgaa tcgttttttt gaactgcgtg
     1381 cgatttcacc atgttttgct tgaagctcgt gcgaatcaat cctggctcca ctgcgaatac
     1441 ttgaattcca aaaggcttca cttcctggcg gagactttct gtgaatgctt cgatggcata
     1501 cttggaagca gtgtatgcgg agccagttgg aatcgtaatt cttccgagga tggacgacac
     1561 gttgatgatg gtgccggatc tgcgctcacg catggaagga agaacagctc tggccatggc
     1621 cagacaaccg aagtaatttg tttccatttg cgcgcggatt tgttcttcgc tgagttcttc
     1681 gaacgcacca tatacaccaa agcccgcatt gtttacgagc acatctatgt tgccgcactg
     1741 ctggaggatc gtttcggatg cgcggcgcac gtcttctgga ttggtaacgt ccagctggat
     1801 gggccttatg ttctcagacg aaggagcgcg agagagttct ctgactccag caaatacagt
     1861 gtctccggcc tgagctgcgc gtagcgcaag tgctcgaccg atccccgacg tggctcccgt
     1921 caccagatag actctgtttt tcatgcctgg tggaagagtt ccacaatcac tctcgcagga
     1981 aagtcaaaaa ctgtctcagc tctaaaatct gctttacacg aaaaggccca tttgcatttc
     2041 atacattttg gccgctcctg ggtcataatg gggacttagc tcagctggga gagcgtctgc
     2101 cttgcacgca gaaggtcacc ggttcgatcc cggtagtctc cacattcact ttcatcagcc
     2161 aaccacttcc gaatgagtgc aagacttcgg agtcgagtcg gcttctttcc ttcaaccaga
     2221 ttactgccag atcacaggac tccaggccct gccgtactga ctctccctgg tctttgagac
     2281 ccgcatctca agatcgccta acggtttccc tgacgatacc ctgagaccag cccggatgaa
     2341 tccaggcctg gacggaagat caggccgaat cgagtgcccg ccacaggcca aacctgcccg
     2401 agagcagggg aaaaagtact tttcagataa cggtatgtgt tcgcaaagtt cttcgaactc
     2461 tttatggcag cagagaatct caaagacata cgtaagctag tcaaagagtt tctctggaag
     2521 tctttgaata cggaccagat gaattacgtc gccaaggaag cggacccgcg ctttgatctt
     2581 ggaaaggttt ccggctttgg tcacagtatc gttgttacgc gacagaccgc cgcagacgtg
     2641 attgtagatt attttgcaac agaagatgca tttctccatc tgtttgcgac tcttcttttc
     2701 atggaaggca agaacgcaag cggcggcgtg attcactttg tgaatccaga tccaatcatt
     2761 aagaaattgc gcgaacgtca gtggatctat gatccatctg caggcagatt catcaagagc
     2821 cagagccaga ctcgtaccgc tgactgggga tttctccagg aaggaagtga gtattcgctc
     2881 gtgtttggtt ccatagacat tgttgggagt tctgctcttg ccagaaccaa tgttcccgtg
     2941 gacgttgaaa atactctcgt tcgtatgcgc gcctacttgc aggatcaaat tgaagccagg
     3001 aacggacgca tttggtactg gcatggcgat ggaggcgtga tggcctttta tggcgcggac
     3061 gccgcatcgc gagcagtcat ttcatctgtt gccattctgg cctatctgcc gatttttaat
     3121 ctgactcaaa acgaactgag acctgagaac gatgtttcgc ttcgcatcgg tcttcacgca
     3181 ggcctcgcag tctacagaag tgacacggaa aaaatcctga gtccggacat tcgcttcgcg
     3241 gaagaagtgg aaaagcactg cgcggaaaca aatacaatcg caataacaga acgcactgta
     3301 aattctcttc ctcctgaagt gcgtcgccag ttcaatccgg ctggtgaaca ttccgcaaat
     3361 cgaatctacg tttacaggcc ggtgtgatgc aggcatatat tctcgaaccg gactcgaata
     3421 ttcaggaata ctatcgcaac
//
LOCUS       DAORFF010000438         3434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_17495_length_3434_cov_3.299793, whole genome shotgun sequence.
ACCESSION   DAORFF010000438 DAORFF010000000
VERSION     DAORFF010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17495_length_3434_cov_3.299793"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKK76_15160"
     CDS             complement(<1..245)
                     /locus_tag="PKK76_15160"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNL69870.1"
                     /translation="MTIRFVLASASPARRKLLDSAGIPHIAMASDFDEDSVKAEPMLF
                     VQILAKAKAETILAKIDSPDGSTHLPGLADNFFVLGC"
     gene            complement(242..889)
                     /locus_tag="PKK76_15165"
     CDS             complement(242..889)
                     /locus_tag="PKK76_15165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNL69871.1"
                     /translation="MFLIFRADLTAHVELQEHETRHARARRLTDGAPISLGDGLGRRV
                     RGQLTSLRTAQFDLTTVETQFEPSRILYSAVPEPSRMEWLLQKGTELGMTAFVPVRFV
                     RSENRSVRDDRARRIIEEAAVQSRRFVLPKMHPAMDLEEACKVSKNPIVLLPEAEKLL
                     ATEGSTEEISFFVGPEGGFSPDEISLLSKFSQRRLSRTILRVETAAICALSRCLL"
     gene            946..1029
                     /locus_tag="PKK76_15170"
     tRNA            946..1029
                     /locus_tag="PKK76_15170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Leu,seq:caa)
     gene            complement(1031..1198)
                     /locus_tag="PKK76_15175"
     CDS             complement(1031..1198)
                     /locus_tag="PKK76_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69872.1"
                     /translation="MPKLLELERLARQIDPDLECLVEYRGEDDRTGIQFRDCIVDYTA
                     CQNQTCIKKLM"
     gene            complement(1270..2325)
                     /locus_tag="PKK76_15180"
     CDS             complement(1270..2325)
                     /locus_tag="PKK76_15180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNL69873.1"
                     /translation="MSFSLPDEIQHYEKKIYDQRQLIEISRALNSTLDYNYLIQAILD
                     ICLAQVQTMQAALFLMPDMDSDFIGPVDGYRGFVFTKPEEEYRIQVQSPLVQLMETTN
                     KSLKLAELDERISSHQPVPSLRELGVELMVPLKAKGKVIGLILLGEKAVGGDYPDNEK
                     SFLFDLASLGGIAVENARLYERATVDMMTSLKNHAYFQSRFREEREKALKRRHPLSLL
                     LTDVDKFKNFNDTHGHQAGDEVLKAVARVLIESARRTDFAARYGGEEFCLVMPGADEK
                     TALAMGEQIRQTVEAMTVIHEGKEMKVTMSIGVSCFDPDLDARSNKTMIERSDKALYA
                     CKHGGRNQVRAYSKDMG"
     gene            2416..2488
                     /locus_tag="PKK76_15185"
     tRNA            2416..2488
                     /locus_tag="PKK76_15185"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2449..2451,aa:Met,seq:cat)
     gene            2518..2591
                     /locus_tag="PKK76_15190"
     tRNA            2518..2591
                     /locus_tag="PKK76_15190"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2552..2554,aa:Met,seq:cat)
     gene            complement(2589..>3434)
                     /locus_tag="PKK76_15195"
     CDS             complement(2589..>3434)
                     /locus_tag="PKK76_15195"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNL69874.1"
                     /translation="IRLSAGFNRSIRTECTRMMATPAQRSMGFYVSCDCCAVNSSLIE
                     RNKNRASVVAKLPHHPGKTYWPSRIVTWLGQNFMRLFFKIKVEGHGNFPESGPLLILA
                     KHQRLEDIPLGLGAVCTAMKRRDQWCVMKHSLADPKYFGFFLKCGGIPIDRRDPQKSK
                     GFLLLARHKLHEGNTVVIFPEQTFFPSKMGKGKVPGFRFVAGRPDSPIQVGCIGFTYK
                     KGIIRTEVTMRLGKTTTPYTKHDDAEHFLHDRMLEIAELSSLHYPHPRPEGRKAGADM
                     EEEEN"
BASE COUNT          834 a          900 c          840 g          860 t
ORIGIN      
        1 tcacagccaa gcacgaagaa gttgtccgcc aggccaggaa ggtgggtaga tccatccggc
       61 gaatcgatct tcgcaagaat cgtctctgct tttgctttcg ccagaatctg gacaaacagc
      121 atgggttccg ctttcaccga atcttcatca aaatcactgg ccatcgcaat gtgcggaatc
      181 ccggccgaat ccagcagctt tctcctcgca ggagaagcgg atgcaagtac aaagcgaatc
      241 gtcataacag acaccgtgaa agtgcacaga tcgcggctgt ctctacgcga agtatggttc
      301 gactcaacct gcgctgagaa aacttggaaa gaagagagat ttcatccggc gagaatccac
      361 cttcgggacc aacgaagaag gaaatctcct cagtagatcc ctctgtcgcg agaagtttct
      421 ccgcctcggg caaaagcacg attggatttt tcgaaacctt gcatgcctcc tcgagatcca
      481 tcgcaggatg catctttgga agaacaaagc gacgggattg aacggctgct tcttctatga
      541 ttcttcgcgc acgatcgtct ctgacagatc ggttttccga acgaacaaaa cgaaccggaa
      601 caaacgcggt catccccagc tctgttccct tttgtagaag ccattccatc ctcgaaggtt
      661 ctggaacggc agagtacaga atgcgcgaag gctcgaattg agtttcaaca gtggtcaggt
      721 caaattgagc ggtgcgaaga gatgtgagtt gtccgcgcac gcggcgtccc agaccgtctc
      781 ccaggctaat gggtgcgccg tctgttagcc gtcgtgcacg cgcatgcctg gtctcatgtt
      841 cctgcagctc tacgtgcgca gtgagatcgg cgcgaaaaat caaaaacata ataactgatg
      901 cataaatggt ttacagtaca ggccgtgcgg ggacgttgat tctacgccga agtggtggaa
      961 ttggtagaca cagtggattc aaaatccacc gggagcaatc ccttgtgggt tcaagtccca
     1021 ccttcggtac tcacatcaac ttcttaatgc aggtctgatt ctggcaggcc gtgtaatcca
     1081 cgatacaatc acggaactgg attccagtcc tgtcatcttc accgcggtat tcaaccaggc
     1141 attccagatc cgggtcgatc tgccgtgcca gcctttctag ttcgaggagc tttggcataa
     1201 acatccctcc gtggacgttc attcgtgcca ggaccttttg gcccttctgt atgtagttag
     1261 attatcggat cagcccatgt ctttgctgta ggcccggacc tgatttcggc cgccatgctt
     1321 acaagcgtaa agagccttat ccgagcgttc aatcatggtt ttattgcttc tggcgtccag
     1381 atccggatca aagcaggaaa caccgatcga catagtgacc ttcatttcct tgccttcgtg
     1441 aatcacagtc attgcttcca cggtctggcg aatttgttca cccatggcga gcgccgtttt
     1501 ttcatccgca ccaggcatca cgagacagaa ttcttccccg ccgtatctcg cagcaaaatc
     1561 ggttctacgc gcagattcaa tgagtaccct ggccacagcc ttgagcactt cgtctcctgc
     1621 ctggtgacca tgagtgtcat tgaaattctt aaacttatct acgtccgtaa gaagaagaga
     1681 gagaggatgt cttctcttga gagctttttc cctttcttcg cgaaagcgac tctggaagta
     1741 tgcatgattc ttaaggctgg tcatcatgtc tactgtggct cgttcataca aacgagcgtt
     1801 ttcaaccgcg atgccgccga gcgatgccag atcaaaaagg aagctttttt cgttgtccgg
     1861 ataatctcca ccaaccgctt tttctccgag gagaatcaaa ccaatcacct tcccttttgc
     1921 tttcagaggg accatgagtt caacgcccag ctctctcaaa gaaggcacag gctgatgcga
     1981 tgagatgcgc tcatcgagtt ctgcgagttt cagagacttg tttgtggtct ccatgagttg
     2041 aacgagagga gactgaacct gaatgcgata ttcctcttcc ggctttgtga aaacaaaacc
     2101 gcgatagcca tccacaggac cgataaaatc ggaatccata tccggcatca gaaacagagc
     2161 agcctgcatg gtctggacct gagccagaca aatgtcgaga attgcttgaa tgagataatt
     2221 gtaatctaga gtagaattga gcgctctgga aatctcaatg agctgccgtt gatcataaat
     2281 cttcttttcg tagtgctgaa tttcatctgg aagtgagaac gacatggcgg ataaggttta
     2341 tacacgcctg cccaagggtc aacacctaaa aatgatggac aagaagcccc gcttttcgaa
     2401 tcctagtcat aacaccgcgc ggtagagcag ttggtagctc gttgggctca taacccaaag
     2461 gtcacaggtt caaatcctgt ccgcgctaat tacgggcccg agagggcctt ttttgtcggc
     2521 gaagtagctc agctggttag agcgtcggaa tcataatccg caggtccggg gttcgagtcc
     2581 ctgcttcgct aattttcctc ttcttccata tcagcccctg ccttgcgtcc ttcagggcgc
     2641 gggtggggat aatgcagact gctcagttcc gctatctcaa gcatgcgatc atgcagaaag
     2701 tgttccgcat catcgtgttt ggtgtaaggt gtggttgtct tgccaaggcg catggtcact
     2761 tccgtgcgaa tgattccttt cttataagtg aaaccaatgc atcccacctg aatcggacta
     2821 tccggtcttc ctgctacaaa tcgaaagcca ggaaccttgc ctttgcccat cttgctcggg
     2881 aagaaagtct gctctggaaa aatcacgact gtgtttcctt cgtgaagctt gtggcgggcc
     2941 aataacagga aacccttgct cttctgcgga tccctcctgt cgatcgggat gccgccgcat
     3001 ttcaaaaaga atccgaagta tttaggatcc gccagagagt gcttcatgac acaccattga
     3061 tctcttctct tcatcgcagt gcagaccgca ccgagaccca gtgggatatc ttcgagcctc
     3121 tggtgcttgg ccagaatcaa gagaggaccg gattccggga aattgccatg cccttctacc
     3181 ttgatcttga agaaaagccg catgaaattc tggccaagcc aggttacgat gcgagaaggc
     3241 cagtatgttt tgccgggatg gtgaggaagt ttagccacga ccgatgctcg atttttgttc
     3301 ctttcgatca agctcgaatt gacagcacag caatcgcacg agacataaaa tcccatgctt
     3361 ctctgggccg gcgttgccat cattctcgtg cactcagtcc tgatgcttct gttgaagccg
     3421 gcactcaatc gaat
//
LOCUS       DAORFF010000439         3428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_17548_length_3428_cov_3.233323, whole genome shotgun sequence.
ACCESSION   DAORFF010000439 DAORFF010000000
VERSION     DAORFF010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17548_length_3428_cov_3.233323"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PKK76_15200"
     CDS             complement(<1..816)
                     /locus_tag="PKK76_15200"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL69875.1"
                     /translation="MTRFTLDEPQSSSPLSGDPLKARRRFRLYVLMGVLGLVLFPALV
                     WIGYYFYKKQTQAPQTALDEKIQRVDLEGLKDKIYLPDEQVSPPLQKAISLYKENFKD
                     QAKREFNNFLNTGASDHEKAIALTYLAVMDMESEKYPVAEHNLLRALNKDEKFVPALV
                     NLAITEQKLGKNGEARQYAEKARELAPGDPRVAALLGNLMAAGQDLSGAIDAYRQGIK
                     QNPDDATLTYNLAVSLLRKQNYEEAILYFSKVMQIAPGTEIAAKSSAQLGQVYF"
     gene            complement(813..1256)
                     /gene="nusB"
                     /locus_tag="PKK76_15205"
     CDS             complement(813..1256)
                     /gene="nusB"
                     /locus_tag="PKK76_15205"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNL69876.1"
                     /translation="MKGRHKNSHAREVALQGLYQAEIGSTPVAEILRFKWLNESLEPE
                     EKTLASELISGVLDHAAALDQVLSAFSNIDFTQISSIVRCIIKMGIYEMRVTGRDPAI
                     VMDDLLNLARRYDGEQAVPFVNGVLDRYRKEMQEAAVKRMTQDES"
     gene            complement(1253..1729)
                     /gene="ribH"
                     /locus_tag="PKK76_15210"
     CDS             complement(1253..1729)
                     /gene="ribH"
                     /locus_tag="PKK76_15210"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNL69877.1"
                     /translation="MSQGGFASGPLDGKGQRHAVLVARFNSDITEALKEGAIAAFARA
                     GVKAEDVEIFYVPGAFEFGSAAKAIALTGRYSAVTCLGCVIQGDTDHHLYVSGEAARL
                     IGQAAYDTGIPVIFGVLTTHTHKQALERAGATGNKGQEAADTAIEMALLLKRIRGS"
     gene            complement(1726..3003)
                     /locus_tag="PKK76_15215"
     CDS             complement(1726..3003)
                     /locus_tag="PKK76_15215"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNL69878.1"
                     /translation="MNATSPNKPFAYAGLVFTLIGIGISGLLVCKHVFPEFCSSSYGC
                     TIGGVDGCSELGSSRYSKIFGIPIAIPGFFFYATSFALFGLTLRETNKDKSAALASLL
                     FTVAVFGAVFDVVLGYINFTKLVVPCRLCIYTYFCTAGFLICAIGIYVMSKKQNQNAK
                     SLSVKEEARIMMPAAGAGLAATAVLFVFLFGASKISGKPGASGDLLPPDQTADFLKDF
                     RALSKVDISTAGLDGYEGDENAYIIIQKFADFMCPHCYHTSKELQKAQARWPGRIRIY
                     YRHFPLDGACNPVVQRKDPNYGPMRCNGAKAALCGGEQKIFPALYHGIFELQNEPEGI
                     SIPNLQAVTEKSGGNWVKLNACMNSPKTMAALLRDVKDAEAIKLNSTPTLIVQDRLLP
                     PGTPDEKYFMSLMDALVFEKEGAKGYESYGRSK"
     gene            3156..>3428
                     /locus_tag="PKK76_15220"
     CDS             3156..>3428
                     /locus_tag="PKK76_15220"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNL69879.1"
                     /translation="MIHFDWSYLVMVGPFMLLSLWASFRVKRTFAKWNQEPNSAGYSG
                     AEVARRVLDASGLSNVKIERVEGELTDHYDPSSKTLRLSDSTFDSTS"
BASE COUNT          805 a          868 c          887 g          868 t
ORIGIN      
        1 gaaataaacc tgccccagtt gcgcggaaga ttttgctgcg atctcagttc caggtgcaat
       61 ttgcattact ttagaaaaat ataggatcgc ttcttcgtag ttctgctttc tcagaagaga
      121 aacagccagg ttgtatgtta gagttgcgtc gtccggattc tgtttgatcc cctgacgata
      181 ggcgtcaatg gcaccgctta gatcctggcc tgccgccatc agattcccga gtagagctgc
      241 cacacgcgga tcccctggag ccagttcacg tgctttctct gcgtactgcc tggcctctcc
      301 gttttttcca agcttctgtt ctgtgatggc aaggttgacc agggccggaa caaacttttc
      361 gtctttgttt agagcgcgaa gcaggttgtg ttctgccacg ggatactttt ctgattccat
      421 atccatcacg gccagatacg tcagtgcgat cgccttttca tgatcactgg cgccggtgtt
      481 tagaaaattg ttgaattcgc gttttgcctg atctttgaag ttttccttgt acagtgaaat
      541 tgctttttga agtggaggag aaacctgctc atctggcaga tagattttgt cttttaaacc
      601 ctccagatcc acacgctgga tcttttcgtc tagagctgtc tgtggtgcct gggtttgctt
      661 cttatagaag taatatccaa tccagaccag tgccgggaaa agcacgagtc caagcacacc
      721 catgagaacg tagagtctga atcggcgccg tgccttgaga ggatcgccgg atagcggaga
      781 agaggattgt ggttcgtcta aagtaaagcg cgtcatgatt cgtcctgggt catgcgcttc
      841 acggcggcct cctgcatttc tttgcgatac cggtccagca ccccgtttac gaagggtacg
      901 gcctgctctc cgtcataacg acgcgcaaga ttcagaagat cgtccattac tatcgccggg
      961 tcacgcccgg tgacgcgcat ctcataaatg cccattttta ttatacatcg gacgattgag
     1021 gaaatctgcg tgaaatcaat gttggagaaa gcggacagaa cctggtctag agcggccgca
     1081 tggtccagaa ctccgctgat cagctcgctt gccagggtct tttcctctgg ttccagggac
     1141 tcattgagcc atttgaagcg cagaatctca gctacaggag tgctcccgat ctctgcctga
     1201 tagaggcctt gcagggcgac ttccctggcg tgggaattct tatggcgacc cttcatgagc
     1261 cgcgaatccg cttcagcagg agcgccatct ctatggctgt gtctgccgct tcctgacctt
     1321 tgtttccggt ggcccctgct ctttccaggg cctgcttgtg agtgtgagtg gtcagaactc
     1381 caaagatgac cgggatgcct gtgtcgtatg ctgcctggcc aatgagccgg gctgcctctc
     1441 ctgaaacata gagatgatgg tccgtgtctc cctggatgac acatccaaga caggtcacgg
     1501 ccgaataacg tccagtcagc gcgattgcct ttgcagcgct cccgaattca aaggcacctg
     1561 gaacgtagaa aatttctacg tcctccgctt tgactcctgc gcgagcgaaa gcagcgatcg
     1621 ccccttcttt gagtgcttct gtgatatcag aattgaaacg cgccacaaga acggcatgcc
     1681 tctgtccttt tccatccaga ggacccgaag caaagccgcc ctgactcatt tcgatctgcc
     1741 gtaagactcg taacctttcg cgccttcttt ttcaaagacg agagcatcca tgagactcat
     1801 gaaatatttt tcatccggag tgcctggtgg gaggagtctg tcctgaacga tgagagttgg
     1861 agtcgaattc agtttgattg cctctgcatc tttcacgtcg cgaaggagtg cagccattgt
     1921 ttttggagaa ttcatgcatg cattgagctt cacccagttg ccaccgctct tctctgtgac
     1981 tgcctgaaga tttggaatag agattccttc cggttcattc tgaagttcaa aaattccgtg
     2041 atagagtgca gggaaaattt tctgctcacc gccgcagagt gccgcttttg ctccgttgca
     2101 tctcatgggt ccgtagttag gatccttgcg ctgcactaca ggattgcacg ctccatccag
     2161 aggaaagtga cgatagtaaa tgcgaattct gcctggccag cgagcctgtg ccttttgcag
     2221 ttccttgctt gtgtggtagc aatgagggca catgaaatct gcgaacttct gaatgatgat
     2281 gtatgcgttt tcatctccct catatccgtc gaggccagca gtcgagatat caactttgct
     2341 gagtgcgcga aagtctttga ggaaatctgc ggtctgatcc ggaggaagaa gatctccgga
     2401 tgcaccgggc ttacctgaaa ttttggacgc accaaacaga aaaacaaaca ggacagcggt
     2461 agccgcaagg ccggcacctg ctgcaggcat catgatgcgg gcttcttcct tcacagaaag
     2521 agattttgcg ttttgatttt gtttcttaga catgacatag atcccgatgg cacagatgag
     2581 aaaaccagcc gtgcaaaaat aagtatagat gcatagacga cagggaacca cgagctttgt
     2641 gaaattgatg tagccgagca ctacatcgaa gactgcacca aacactgcca cggtaaacag
     2701 gagagatgcg agcgccgcgg atttatcctt gtttgtttcg cgcagagtta gaccgaaaag
     2761 cgcgaaagaa gttgcataga aaaagaaacc ggggatggca atgggaatcc cgaagatctt
     2821 ggagtaacgg cttgatccaa gttctgagca accgtccacc cctccaatcg tgcacccgta
     2881 tgaggaagag cagaattccg gaaatacgtg tttgcacacc aggaggccag aaatcccaat
     2941 gccgatcagg gtaaacacaa gcccggcata ggcaaatggc ttgtttggag aagtcgcgtt
     3001 catgaggcca cgatttatcc ggccgcaggc cattaaagaa aaaaccgggg acaactctcg
     3061 cgccggaata aaatttccag gcaaatcggc agattttgaa gtggtcgctt acccgaaggc
     3121 gttctatttt gtggccctga atctaggagg ttcgtatgat ccattttgat tggtcctatc
     3181 tggtaatggt cgggccattt atgctgctca gcctgtgggc cagcttccgc gtaaaacgca
     3241 cgttcgcgaa gtggaaccag gagccaaact cggctggcta cagcggggcg gaagtcgctc
     3301 gcagagtgct ggacgcgtcc ggcctcagca acgtaaaaat cgaaagagtg gaaggcgaac
     3361 ttacggatca ttatgatcca tcttccaaga ctcttcgcct gtctgattcc acgttcgatt
     3421 ccacaagc
//
LOCUS       DAORFF010000440         3423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_17590_length_3423_cov_3.860451, whole genome shotgun sequence.
ACCESSION   DAORFF010000440 DAORFF010000000
VERSION     DAORFF010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17590_length_3423_cov_3.860451"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PKK76_15225"
     CDS             complement(<1..601)
                     /locus_tag="PKK76_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNL69880.1"
                     /translation="MAEETKSETPASGLDFAHLHLHTTYSLLDGAIRIPDLMKYCKEN
                     GMSSVAMTDHGNMFGAIQFYKEAVKQGIKPIIGNEFYVAPGSRHEKKHMERLADGNNY
                     HLILLAQNQTGYANLIRLTSRSYTEGFYKKPRIDYELLEKHSEGLICLTACLGGEVQN
                     KIINGREPEARDLAGRMKEIFGEDRFYLEIQNHGIPEERT"
     gene            656..1483
                     /locus_tag="PKK76_15230"
     CDS             656..1483
                     /locus_tag="PKK76_15230"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL69881.1"
                     /translation="MRVSATALKLAIPRHYIDYWRKTGLLSKNTDLSFTDLRRIQLIW
                     DCTRGSVSLRRIRRILGPAESIPAIAVEHTPSGVFFYQGGETYADPEGQYPLFPVERE
                     VPRVLSFPKKVQKKEDEEIRKLEAEYADALAMSDRRRQVRAISMILQLNPYHSGALIE
                     KGNQAFDQGRWNRAVYFYELAATRNPDCVEALYNTANAYFKQGKYAPAIRYFQDCIDL
                     DPDFPESHFNLAILYMKLGQPGPARAYFEAYLDLDPDSEWGELARQFLEDIQSAAEE"
     gene            complement(1486..2007)
                     /locus_tag="PKK76_15235"
     CDS             complement(1486..2007)
                     /locus_tag="PKK76_15235"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HNL69882.1"
                     /translation="MTLFTLQSSILEIILRTGIVFAALMIFVRIFGKKEIGQIAPNDL
                     VLLLLISNAVQNAMVGADQSVPGGLAAVGTLLALNFLLTFLASRSKSFRSIVEGKAAI
                     LIHRGTVVSENLKKEKVSILELEQALREHGVAAISDVYLAVLELDGSISVLKKDEIPA
                     ETKPHHRMRFLKR"
     gene            2648..3178
                     /locus_tag="PKK76_15240"
     CDS             2648..3178
                     /locus_tag="PKK76_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69883.1"
                     /translation="MMQVSRFRAGCKLKANVFTRLAVTFAAFCAIGLLANPIRIWEPA
                     LIILLGERSNVGRLQQWIALKGGIWPFLAGFLSVEIHWLEGFAFVVLALLLAGAFARS
                     INGCLWISAACSILAAAWPGYLRPVVFGGIHRFDEISTYITIFLRMESGFFLLLILFL
                     HALHSRRRGRVSHFDS"
     gene            3168..>3423
                     /locus_tag="PKK76_15245"
     CDS             3168..>3423
                     /locus_tag="PKK76_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69884.1"
                     /translation="MTVSLWTGSPGAAADSICAEAARDDHGVLSPNIGGLTVSSNGNV
                     NGTIGTDTPYGAVGVSITNGQLGASLFGDLGNFNYANGQIG"
BASE COUNT          773 a          801 c          934 g          915 t
ORIGIN      
        1 ccgttctttc ttctggaatt ccgtggttct ggatttccag atagaatcga tcttctccaa
       61 agatttcctt cattctgcca gccaggtctc ttgcttctgg ctcgcgtccg tttatgattt
      121 tattttgaac ttctccgccc aggcatgcag tcaggcagat caatccttcg ctgtgttttt
      181 cgagtagttc gtagtcgatg cgcggctttt tgtagaatcc ttctgtgtaa ctacggcttg
      241 tgaggcgaat gaggttggcg taacctgttt gattttgagc gagcagaatc agatggtagt
      301 tgtttccatc tgccaggcgc tccatgtgtt ttttctcatg acggcttcca ggagcgacgt
      361 agaattcatt tccgatgatt ggcttgattc cctgtttcac tgcctctttg tagaactgaa
      421 tggcaccaaa catattgccg tggtctgtca tcgccacgga agacattccg ttttctttgc
      481 agtatttcat cagatccgga attcggatgg ctccgtcgag gaggctgtaa gtagtatgaa
      541 gatggaggtg ggcgaaatcg agtccggaag ccggggtctc tgattttgtt tcttcagcca
      601 tgtgacataa gaaaaaagac tggaaaaacg agcaagcaat gcctgaaatg tttgtatgcg
      661 ggtttcggcc acggcactca agcttgccat tccgcgccat tacattgatt actggcgtaa
      721 aaccgggctt ttaagcaaga acaccgattt aagcttcacg gacctgcgca gaatccagct
      781 catctgggac tgcaccaggg gatccgtaag cctgaggcgc atcagacgca ttctaggacc
      841 cgctgaatcg attcctgcga tcgcagtcga gcacactccg tccggggttt tcttttacca
      901 gggcggggaa acgtacgcag atccagaagg gcagtatcca ctttttccag tggagagaga
      961 agtgcctcgc gtgctttctt ttccaaagaa agttcagaag aaagaagacg aagaaattcg
     1021 caaactggaa gcagaatatg cggacgctct tgccatgtcg gatcgccgca ggcaggtgcg
     1081 agcgatttcc atgattctgc agttgaatcc atatcacagc ggtgcgctca tagagaaggg
     1141 aaatcaagcg ttcgatcagg gaagatggaa tcgcgccgta tatttttatg aactcgcagc
     1201 cactcgaaat cctgattgcg tggaagctct ttacaatact gcaaacgctt acttcaagca
     1261 gggaaaatac gcgccagcca ttcgctactt ccaggactgc attgatctgg atccggattt
     1321 tccggaatcg catttcaatc tggccatact ctacatgaag cttggccagc caggtcctgc
     1381 gcgcgcttac tttgaagcgt atctggattt agatcctgat tcagagtggg gagagctcgc
     1441 gcgtcaattt ctggaagaca ttcaatctgc agcagaggaa tagtttcatc tttttaggaa
     1501 tcgcattctg tgatgaggtt ttgtctctgc cggtatttca tctttcttta agacgctgat
     1561 agagccgtcc agttccagca ctgccagata cacatcgctg atcgccgcca caccatgttc
     1621 gcgcagtgcc tgttccaatt ccagaatcga cactttttcc ttttttagat tttctgaaac
     1681 aacggtaccc cggtgaatca ggatggctgc ttttccttct acgatgctcc gaaaactctt
     1741 ggagcgagag gcaagaaaag taagcagaaa attgagtgcg agcagggttc ccacagctgc
     1801 gaggccgcca ggcacagact gatctgcacc caccattgca ttttgaactg cattggaaat
     1861 cagaaggagc aataccagat cgttcggggc tatctgcccg atttcttttt taccaaagat
     1921 gcgcacgaag atcatgagtg cggcaaagac aatgcctgtg cgaaggatga tttctagaat
     1981 agaactttgc agtgtgaaga gagtcatgat aagatcaagc ttgccgccga tccgttatgc
     2041 ggcatagcaa tttgcggcgt ccgggtggag gttgccgagc cggcatagat ccatagggga
     2101 ggcgtatggt tctcgaggga tcgattcgct gcgcgaatcg tttatgaaat gcgccgcttc
     2161 ttaaatttga aagcctcgaa ggcctctcgc ggtctaaaag acggctttgc gcctgcgcgc
     2221 tgtctccgag cgtgcatatg agattcaagc actcgagatc atccggactc aaggggacat
     2281 ggagagccag ctgaggtatt tgtgggcgga gcccatgcgc acgatttttc cattgcggag
     2341 attgggcaag acgtacaagc tgagtccatt acatgcagta ggttccgttt gcggggaggt
     2401 ggccccggaa atactgacgg tgtcgataga tcgatggagg gcgcggggat gcggcgaaac
     2461 cagttcatga ttctagcttg tatctttgct ttcgcgagcg cgagaacagc cagtctttat
     2521 gcgagtcgtg caaaaatgtc cgatccggca cgggattctc tacataagtg gcgaaagtac
     2581 ttgtccgatg taaagaacca gatggatcat ggcggcctaa acgatccttg gcgggatagc
     2641 agcgcgattg atgcaggttt cgcgctttcg ggctggttgt aagttgaaag cgaatgtgtt
     2701 cacacgcctt gcggtaacat ttgcggcatt ctgtgccatt gggcttctcg ctaatccgat
     2761 ccgcatctgg gaacccgcct tgattatttt gctgggcgag cggtcaaacg tgggaagatt
     2821 gcagcaatgg atagcattga aaggcgggat ctggcctttc ttggcggggt ttcttagcgt
     2881 ggagatacat tggctggaag gttttgcgtt cgttgtattg gctttgttgc ttgctggtgc
     2941 atttgcgcgg tcgatcaatg gttgcttatg gattagtgct gcgtgctcta tcttggccgc
     3001 tgcttggccc ggttatctgc gccccgttgt atttggcggc attcatcgat ttgatgaaat
     3061 atcgacgtac attacgatct tcctgaggat ggaatccggt ttcttccttc ttctgatatt
     3121 gttccttcac gcgcttcatt cgcggcggcg cgggagagta tcgcactttg acagttagcc
     3181 tttggactgg atccccgggc gctgcagcag attctatttg cgcggaggcc gcacgagatg
     3241 atcacggagt actatcccca aacattggcg gcctcacagt gtcgtctaac ggtaacgtga
     3301 acggaacaat cgggacggat acaccgtatg gtgcagtggg cgtgagcatc accaatggcc
     3361 agctgggagc atctctattc ggggatcttg ggaacttcaa ttatgcgaat ggacagatcg
     3421 gcg
//
LOCUS       DAORFF010000441         3418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_17636_length_3418_cov_3.523045, whole genome shotgun sequence.
ACCESSION   DAORFF010000441 DAORFF010000000
VERSION     DAORFF010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17636_length_3418_cov_3.523045"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..793)
                     /locus_tag="PKK76_15250"
     CDS             complement(233..793)
                     /locus_tag="PKK76_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HNL69885.1"
                     /translation="MKKPILLKRYANRRLYDAETSRTLTLEDVAARIRSGHEIKVVEN
                     ATGEDITAYILGQTFLKVSLDRDHPEFSSFLLTALIREISGNVSHFFHRLVRGGIGFD
                     NLTRERMESIAARLVEKGELQIAERSEYVDRLMNQLHESRLDQIEKTTEAGKDRLREK
                     LNRDDRIEELSVKLQELSGFLQDLRK"
     gene            879..2006
                     /gene="pgk"
                     /locus_tag="PKK76_15255"
     CDS             879..2006
                     /gene="pgk"
                     /locus_tag="PKK76_15255"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNL69886.1"
                     /translation="MQRIETLSVSNQKVFVRADLSGLGDPPSGKALSRILPVVEILLG
                     KQAGVVLAGHDSNQNTFAPLADAIGKSLGRPVHLVEPGDMKQRWSTLKAGQVILLENL
                     SKYDGETKGENAFAEQIGGGADLYVSDCFAALALPYASITKLTKNKTIAAGPGLHSIL
                     LSLQEINSQKNKPLVSFLGGNDFAKKVDFMPRMLRLSQTALIAGSIAYTFLKSRLVPV
                     GNSIVDNGLEVTAFQLQEKAELEQTQFILPVDHMIGERFSRDAKAKASRDLPDRWMGL
                     DIGPKTIASFEKALKGAGSILWYGTAGAVELPAFQTGTKSLMKFASKLKAPITFLGED
                     TVRMADEFTFASATMIPGSESAIQVLLGKNPPGLALLQKEI"
     gene            2038..2391
                     /gene="secG"
                     /locus_tag="PKK76_15260"
     CDS             2038..2391
                     /gene="secG"
                     /locus_tag="PKK76_15260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNL69887.1"
                     /translation="MAIIQTVILVVFFICCAGLIFLILIQSGKGGSMGIMGGGGSNTA
                     FGSSTVDVVEKAAFWFAIAFFVLAILAAIAFADSGPRVPEKDAQTTEVPATQGTPANQ
                     ANPAPANPAPAPAQP"
     gene            2492..>3418
                     /locus_tag="PKK76_15265"
     CDS             2492..>3418
                     /locus_tag="PKK76_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69888.1"
                     /translation="MKSKLITALAVICAAGVLTAEPKVIDRIAEAKDYPDLQNTEKEQ
                     DNNIQDAYKRLTAYAPLVALAAKDRANSFKERAEPLRFEHTFRNVKYTPRNTYVRYVK
                     ESDKAPGDVMLVGLGDLKTIEDLIAQKTAQANAAGMPNGEQIAPFKFGNRDGVELTQF
                     QFIIREDADKIRAIGSRRKSVILFYKPAAAATPDTIGAQDLDLVVTRIVEDDYDAGVK
                     DVEVIIDPSPSTPGTNDVITLHRYNYKVTTCAILGTMSNTPNYPHRAAFKQKFYARLM
                     DHFDTLYRMVDTYAKRDGNDVNEKTIDRMNHSM"
BASE COUNT          879 a          891 c          831 g          817 t
ORIGIN      
        1 taaagagaag ctaaatattt ttgatcaaag cttccggcgt cgagattgcg aagtgcttcc
       61 tcgctgcgtg cttgattgaa ttgaatgatg tcatcttccg atcccgcgaa gactggaccg
      121 gccacgatca aaagtgcgat agaaaagctg atttggcgca tgggcattcc tttgtagttt
      181 gtctgtcgga gtcggtcaga cgattcttaa agtgcgcttc aaaaaatgta atctattttc
      241 gcaaatcctg gagaaatccg ctcaattcct ggagcttcac ggaaagctct tctatgcgat
      301 catctctgtt caatttctca cgaagcctgt ctttgccagc ctcggttgtc ttttcaattt
      361 gatccagacg cgactcgtgc agctgattca tcaatctatc tacatactca cttctctctg
      421 caatctgaag ttcccctttc tccaccaggc gagcagcaat gctctccata cgttcgcgag
      481 tgagattatc aaaaccaatt ccaccgcgca cgagcctgtg aaagaagtgg gaaacgttgc
      541 cgctgatttc acggatcaga gcagtgagta gaaaagaact gaattcagga tgatcccggt
      601 ccaggcttac ttttagaaaa gtctgcccca gaatgtatgc agtgatgtct tcccccgttg
      661 cattctcgac tactttgatt tcatgcccgc tgcgaatccg cgctgccaca tcttcgagag
      721 tgagcgttcg actggtctca gcgtcgtaga ggcgccggtt ggcatacctt ttcaataaaa
      781 tgggtttctt cactgtcttt ttgcagtgca ccatgaacgt ttggatcgtc aagctacttt
      841 tgagatgaca gtgggacccc gcgtggagtc atcgcgcgat gcagagaatt gaaactcttt
      901 cggtgtctaa ccagaaggta ttcgtgcgcg cggatctttc cggcctgggc gatccaccat
      961 cggggaaagc actgtcccgc atactgccag tcgtggaaat actcctgggc aaacaggcgg
     1021 gcgtcgttct agcaggccac gattcaaatc aaaacacatt cgctcccctg gcagatgcga
     1081 ttggaaagtc actcggtcgg cccgttcacc tggtagaacc aggcgatatg aagcaaagat
     1141 ggtccactct caaggcgggc caggtgatcc tgctcgaaaa cctcagcaaa tacgacggag
     1201 agacgaaagg ggaaaacgca ttcgcggagc aaattggcgg aggggcggat ctatacgtaa
     1261 gcgattgctt cgcagccctg gcacttccct acgcttccat aaccaaactc acaaaaaaca
     1321 agacgatcgc cgcaggcccc gggctccact ctatcctgct cagtctgcag gaaatcaatt
     1381 cgcagaagaa caaacctctc gtgtcttttc taggtggaaa cgacttcgca aagaaagtag
     1441 acttcatgcc gcgaatgctc agactttcgc agacagcact catcgcaggc agcattgcct
     1501 atacattcct gaaatcgagg cttgtgcccg ttggaaattc catcgtggac aatggactgg
     1561 aagtcaccgc attccagctg caggagaaag cagagctcga gcaaacacag ttcattctcc
     1621 cggtggatca catgattggc gaacgcttct ccagagacgc gaaagcgaaa gcttcgcgcg
     1681 atctaccgga tcgctggatg ggcctggaca tcgggcctaa gaccattgca tcctttgaaa
     1741 aggcactgaa gggcgccggg tccattctat ggtatggaac tgccggagca gtcgagcttc
     1801 cagcatttca aacggggaca aagtccctga tgaaattcgc ctccaaactc aaagcaccga
     1861 tcacattcct cggggaggac acagttcgca tggcagacga gtttactttc gcctcggcca
     1921 cgatgatccc cggtagcgag tccgccattc aggtactttt gggaaagaat ccgcccggat
     1981 tggcccttct gcaaaaagag atttgacata gcgctcaggt gcagatttac tatatatatg
     2041 gccatcattc agactgtcat acttgttgtt ttcttcattt gttgcgctgg tctcatcttc
     2101 ctgatcctga ttcaatccgg aaaaggcgga tccatgggga tcatgggcgg agggggatcg
     2161 aacactgcgt ttggctcttc taccgtagac gtggtagaaa aggcagcttt ctggtttgcc
     2221 atcgcgttct ttgtcctcgc aattctggca gccattgcat ttgccgattc tggtcctcgc
     2281 gttcccgaaa aggatgctca gactacggaa gtcccagcca cgcaggggac tccggccaat
     2341 caggcaaacc ctgccccggc aaatcctgct ccggcgcccg ctcaacctta agcaaaaaat
     2401 tcattttttt tttcgaacgg ctaaatttca ggcagtcacc ctcctataat gatctagata
     2461 tagccttggc atgggaagga agggattttt tatgaagagc aagctcatta cggctctcgc
     2521 ggtgatctgc gctgcaggtg ttctcacagc ggaacccaaa gtcatcgatc gcatagcgga
     2581 agctaaagat tatccagatc tgcaaaatac ggagaaagag caggataaca acattcaaga
     2641 cgcatacaag cgactgacag catatgctcc actcgttgct ttagccgcaa aagaccgtgc
     2701 gaactctttc aaggaaaggg ctgagcctct tcgttttgaa catactttcc ggaacgtcaa
     2761 gtatacccct cgcaatacgt atgtgcgtta tgtcaaagaa agcgacaaag ctcctggcga
     2821 tgtaatgctt gttggactcg gagatctcaa gacaattgag gatcttatcg cacagaagac
     2881 ggcccaggca aatgctgcgg gaatgccaaa tggcgaacag attgctccct tcaagtttgg
     2941 caatcgtgac ggcgttgaat tgactcaatt ccaattcatc atccgcgaag acgcagacaa
     3001 gatccgcgcc atcggatcac gtcgcaagag tgtgattctt ttctataagc cagcagctgc
     3061 tgctacaccg gacaccattg gtgcgcagga tctggatctt gtagtaactc gcatcgtgga
     3121 agatgactat gacgcaggtg taaaagatgt ggaagtgatc attgatccat ccccttctac
     3181 tccgggaacc aatgacgtca tcactctgca tcgctacaat tacaaggtaa ctacctgcgc
     3241 gattctggga accatgtcca acactcccaa ctaccctcat cgcgcggcgt tcaagcaaaa
     3301 gttctacgct cgactgatgg atcactttga tacgctctac cgcatggtag acacatatgc
     3361 aaagcgtgac ggaaacgatg tgaacgaaaa aacaatcgat cgcatgaacc acagcatg
//
LOCUS       DAORFF010000442         3411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_17691_length_3411_cov_4.023540, whole genome shotgun sequence.
ACCESSION   DAORFF010000442 DAORFF010000000
VERSION     DAORFF010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17691_length_3411_cov_4.023540"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="PKK76_15270"
     CDS             complement(<1..1188)
                     /locus_tag="PKK76_15270"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNL69889.1"
                     /translation="MAVSQDIICPFQTVPSLLEARAKETPQKPYLIFQEEEVSYSQFR
                     SSVRKMAAHFQTTGAGHGSRVAILIPNSDHFLYCYFGAMEAGLAAATLNTLLKAEELE
                     FIINDCEAEILCTTPHYRKLLDPVFENLKNIKHVIFTEPGDLPHSALIGDILKGNAEY
                     SDPNVTAADDAAMIYTSGTTGHPKGVVLTHSNVMYNSYVAPRFIDLQAADVSLCIMPL
                     FHVNAQIASMLATMQAGATVVLEEMFKPRTFIKTLKKHRCTTFSGVPTIFNFLNEMKE
                     AEGEDLSFLKACICGAAPMPVEVFQTFERKFGAKIIEGYGLSEGTCVSSLNPLNGVRK
                     IGSIGIPIDGQDMSIWDDDANEMPAGQTGEIVIKGPNVMKEYYRNPEATAKTLHNGWL
                     RTGD"
     gene            1260..1589
                     /locus_tag="PKK76_15275"
     CDS             1260..1589
                     /locus_tag="PKK76_15275"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNL69890.1"
                     /translation="MTLPHTQRLWRMFEMMVAMDIGDEEAYSAYRAGMSPILKSFGGR
                     FRYDFKVSEALQREATHPINRVFALNFPDRISRDAFFSNPDYKKVRSAYFEQAVKART
                     LIAEYDL"
     gene            complement(1599..2048)
                     /locus_tag="PKK76_15280"
     CDS             complement(1599..2048)
                     /locus_tag="PKK76_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69891.1"
                     /translation="MEVKVLEGKIENDYKWNPGTAVGRFLTQMREKEILAVRCTKTSK
                     VFLPPQSWSPYGNIKMDRFIPITAEPKLGAGTIVYQAPWNMPEDLKPPYMLAGISFAG
                     ADSDLIHLVVAPEADLKALKPGDRLRAVWKAETTGTIRDILYFEPVK"
     gene            complement(2057..3265)
                     /locus_tag="PKK76_15285"
     CDS             complement(2057..3265)
                     /locus_tag="PKK76_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69892.1"
                     /translation="MPQKVAITGIGQTRTRLKRKDVNFPELMGEAAQLALQDAGLNAE
                     DIDLVLYGSGPEMFEGVGEPELWGADNTFGVFRPHYRIQTGGTVGGSTTIAGWYMVNS
                     GLANNVLVVSGNKLGESSVQKGLSLVYSPTMGRDFAAGAPSAVANQTRIYMNKYPEAK
                     EEQFARVGTMMRKNALNNPNAALKLPQISEEMMMNMAWLSTPFRLLDSCPTSDAACAM
                     VLQGEKNAEANDRPRAWIQAVSAVSDGVNYINRDWSDPIALKKAAQNCYKAVGITKPL
                     EELDVVEIYDAFTSQHLIWYEGLGLCAPGRAGPDLIETRATSMTGQLPVNPSGGVLSN
                     NSIGASAMIRQAEVALQLMGRAGDRQVANAEVGLAHGWGGAIQFHVVMIMSKEKEIQE
                     SFRKRKEKGR"
BASE COUNT          813 a          876 c          828 g          894 t
ORIGIN      
        1 atcccctgtc ctgagccagc cattgtgaag cgtcttagca gttgcttcag gattacgata
       61 gtactccttc atcacgtttg ggcctttgat tacaatttcg ccagtttgtc ctgcgggcat
      121 ctcgttggca tcatcgtccc agatgctcat atcctggcca tcaattggaa ttccaataga
      181 gccgatcttt cgaacgccgt ttaacgggtt caaagacgag acacaggtgc cttccgaaag
      241 gccataacct tcgatgatct tcgcgccaaa cttacgttca aacgtttgaa agacttctac
      301 cggcatagga gcggcgccgc aaatacaggc tttcaagaag ctgagatctt ccccttccgc
      361 ttccttcatt tcattcaaga aattgaaaat ggtaggtacg ccgctgaatg tagtgcaacg
      421 atgcttcttg agtgtcttga tgaatgtcct tggcttgaac atttcttcga gtacaacggt
      481 agcgcctgcc tgcatggtgg cgagcatgga cgcaatttgc gcgttcacgt gaaaaagagg
      541 catgatgcac agcgacacgt ccgccgcctg gagatcaatg aagcgcggtg ccacatagct
      601 attgtacatc acattggagt gcgtcaacac cacgcccttt ggatggcccg tggtcccaga
      661 cgtgtaaatc atagccgcat cgtcagcagc agtcacattt ggatcagagt attccgcatt
      721 tcctttgaga atatctccga tgagagcgga atgaggcaaa tccccaggtt cagtgaagat
      781 cacatgcttg atattcttta agttttcaaa aacaggatcc agaagttttc tgtagtgagg
      841 cgtggtgcac agtatttctg cttcgcaatc gttgatgata aattcgagtt cctctgcctt
      901 caaaagtgta ttgagtgtcg cggcggcgag tcctgcttcc atggcaccaa agtaacaata
      961 caaaaagtga tccgagttcg ggatgagaat ggcaactctc gatccgtggc ctgctcccgt
     1021 agtttgaaag tgtgccgcca tcttacgaac agaagagcga aattgggaat agcttacttc
     1081 ttcttcctga aaaatgagat atggtttttg cggagtctcc ttcgcgcgtg cttctagaag
     1141 agaaggaact gtttgaaaag gacagatgat gtcttgactg actgccattt cgaaaacatg
     1201 ggattcgtcc tcaggcagtc acgtcctttt tcaaaggaac ccgtgaatgt aggcgatgct
     1261 tgactctgcc tcacactcaa cgtctctggc gtatgtttga aatgatggta gccatggaca
     1321 ttggagatga ggaggcgtat tctgcatacc gtgccggaat gagtccgatt ctgaaatcgt
     1381 tcggaggccg ctttcgttac gatttcaagg tgagcgaggc actgcaaaga gaagcgactc
     1441 atccgatcaa cagggtattt gctttgaatt ttcccgaccg tatctcacgg gacgctttct
     1501 tctcgaaccc ggattataag aaagttcgat ccgcatactt cgaacaggct gtaaaggcca
     1561 gaaccctgat cgcggaatac gatctctaat tcagccgatc acttcacagg ttcaaaatac
     1621 agaatgtcgc ggatggttcc tgtggtctct gctttccaga cagcgcgcaa cctgtctccc
     1681 ggtttgaggg ctttgagatc tgcctccggt gccaccacaa gatgaatcag gtcgctgtcc
     1741 gcgcctgcga atgagatgcc cgcgagcata tagggtggtt tgagatcttc tggcatgttc
     1801 cagggagcct gatacacaat cgtgcctgct ccgagttttg gctctgccgt gatgggaatg
     1861 aagcgatcca tcttaatatt gccgtacgga gaccaggatt gcggcggtag aaacactttc
     1921 gaagtctttg tgcatctaac cgcgagaatt tccttctcac gcatctgggt caaaaaacga
     1981 ccaactgccg tgcccggatt ccatttgtaa tcgttttcga tcttgccttc gagtactttt
     2041 acttccatat gatcgcctaa cgtcctttct ctttgcgttt gcggaacgat tcctgaattt
     2101 ctttttcctt gctcatgatc atgacaacgt ggaactgaat ggcaccgccc catccgtgcg
     2161 cgagaccgac ctctgcattc gccacctgcc tgtcccctgc acggcccatc aattgaagag
     2221 caacttctgc ctgacgaatc atcgcggagg cgccgatgga attgttggac aagacaccgc
     2281 cgctcggatt caccggaagc tggcctgtca tactggtggc gcgagtttca atcaaatcag
     2341 ggcccgctct ccctggagcg catagtccaa gtccttcata ccagatcaga tgctggcttg
     2401 tgaatgcatc gtaaatttcc actacgtcca attcttcgag aggctttgta atgccgacgg
     2461 ccttataaca attttgcgcc gctttcttga gagcaatcgg atccgaccag tcgcgattga
     2521 tgtagttcac tccatctgaa acagctgaca ctgcctgaat ccacgctcgc gggcgatcgt
     2581 ttgcttctgc attcttttct ccctgaagga ccatcgcaca ggcggcgtcg ctcgtcgggc
     2641 aagaatccag aagacgaaac ggagtagaaa gccatgccat gttcatcatc atctcttctg
     2701 aaatctgagg aagtttgagc gcagcattcg gattgttcag agcattcttg cgcatcatgg
     2761 ttccaacgcg cgcgaattgc tcttctttcg cttccggata cttgttcata taaatgcgcg
     2821 tctgattggc aacggcagaa ggagcaccgg cagcaaagtc ccgacccatg gtgggagaat
     2881 acacaaggct caaacctttt tgaaccgatg attctcctaa tttatttcca gaaacaacta
     2941 gaacgttatt cgccaatcca gagtttacca tgtaccaacc ggcgatcgtg gtggaaccac
     3001 ctactgttcc gccagtctgg atgcgatagt gtggacgaaa aacgccaaac gtattgtccg
     3061 cgccccagag ttctggttcg cctacgcctt cgaacatttc gggtccgctt ccataaagga
     3121 ccagatcaat atcctctgca tttaaccctg cgtcttgcaa ggcaagctgt gctgcttctc
     3181 ccatgagctc agggaaattc acatcttttc ttttgagtct ggttctagtc tgaccgatgc
     3241 cggtgatggc aaccttttga ggcatgagcg aatctcagcg tagcgcgccg aacgggaagc
     3301 ctttttcagg agagtttgaa atcggagctg atatcaaaaa cgaccatggt catgtcatct
     3361 tcggcacttc tatcctgcgc aaattcgccc aatcgagaga gaagcaaggt g
//
LOCUS       DAORFF010000443         3403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_17756_length_3403_cov_2.989247, whole genome shotgun sequence.
ACCESSION   DAORFF010000443 DAORFF010000000
VERSION     DAORFF010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17756_length_3403_cov_2.989247"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PKK76_15290"
     CDS             <1..425
                     /locus_tag="PKK76_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-transporting ATPase"
                     /protein_id="HNL69893.1"
                     /translation="LQIDAKMSRITDRAAWSSMIQALAFSALLPLRLARVALQDIVVG
                     LYRPLLRPLQRLAHAILKKQHVILNLVSNPLINLGISFELFLCWLFFYTSLSEIYYFA
                     PVPWHVYLFAFHGMLLIVVFEETKKYFRRRGHELTLLG"
     gene            complement(457..1626)
                     /locus_tag="PKK76_15295"
     CDS             complement(457..1626)
                     /locus_tag="PKK76_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNL69894.1"
                     /translation="MQAINSLPVHSGELWTARQRQMHPIHYTISYRGSFKPELPDFFI
                     NRYLKVPGVVLDPFGGRGTTTLQANLLGHRAVHNDLNPVSVFLARSRQNVPAMKILEK
                     RLDALQLDRRMKPTPEEKERLSPFFHPRTLSEILCLKSELLDNMEDPAMRYIGVTALS
                     RLHGHSDGFFSVYSFPQISIMPGAQRKNNARLGQKPEYRSIRERILRKMKRDLSLPLP
                     GVFNEAARGNRYISENSQDLKTVRTSSVDLIVTSPPFLDKVDYISDNWMRSWFLGLES
                     VTSSIELSVMNSLSEWEDFMRATLFEMGRVLRPGARAVVEVGEVKSRSVLLNLEQVLA
                     GLLPIRVPGGELAVEEILINEQKFTKLSNCWDVKNNEKGTNTNRCLIVRKNLRKA"
     gene            1720..1938
                     /locus_tag="PKK76_15300"
     CDS             1720..1938
                     /locus_tag="PKK76_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNL69895.1"
                     /translation="MHRIVYVKKEDCTSCNQCADSLPSYFRMDDHDTAESHVKNSSIN
                     AAPVPESDWKAVQTEMDECPGECIKWKE"
     gene            2068..2916
                     /locus_tag="PKK76_15305"
     CDS             2068..2916
                     /locus_tag="PKK76_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNL69896.1"
                     /translation="MIINHNISAIYSHRTLKFHDMDESKDIEKLSSGMRINRAGDDAA
                     GLAVSEKMRTQILGLRQAERNAEDGISFVQTTEGFLSETQNMIQRIRVLAVQSANGIY
                     SDEDRQQIQVEVSQLIDEIDRIASQAEFNKMKMLTGAFARLNPTASMWFHIGPNMHQR
                     ERVFINTMTTQALGLRNPTVLTFISISTPDKANSVIGLADEALRKISKQRADLGAYQN
                     RLEHAARGLMNAYENTQAAESRIRDTDMAEQMTSYTRYQILVQAATAMVAQSNMKSQT
                     VLQLLK"
     gene            complement(2967..>3403)
                     /locus_tag="PKK76_15310"
     CDS             complement(2967..>3403)
                     /locus_tag="PKK76_15310"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNL69897.1"
                     /translation="VQERYSLNQRKIDAGLKPYTLKHSIPILAYSPLAQGLLTGAIRS
                     DRVFGEGDRRGGSTIMDPEYISRIEPALDQARAIASSYNVNLSQLAIAWVLSVPGIGA
                     AITGIRDSLHAKENLSALSIAIKREDSIRLKAIFDPFVNNGK"
BASE COUNT          862 a          871 c          837 g          833 t
ORIGIN      
        1 ctctccagat agacgcgaag atgtcgcgca tcacagacag ggctgcctgg tcgtctatga
       61 tccaggcgct cgcattttcc gctttgctcc ctctgcgtct tgcacgagtt gcattgcagg
      121 acattgtggt ggggctatac aggcctctac tgcggcctct gcaaagactc gctcatgcaa
      181 ttctcaaaaa acaacatgtg atcctgaatc tggtctcgaa tcccttgatc aatctgggaa
      241 tctcctttga gctgttcctc tgctggttgt ttttctatac cagcctttca gaaatctact
      301 acttcgcgcc tgtgccgtgg catgtgtatc tcttcgcatt tcacggaatg cttctcattg
      361 tagtgtttga agagacgaag aagtacttca gacgccgtgg gcatgaactc actttgctgg
      421 gctgatctta caccgggaag agtcagggcg tgagaatcaa gccttgcgca ggttctttct
      481 cacaatcaaa caacggtttg tatttgtacc cttttcgttg ttcttcacat cccagcaatt
      541 ggaaagcttt gtgaatttct gttcgttgat cagaatttcc tctacggcaa gctctccgcc
      601 tggcacgcga atcggaagca atcctgcgag cacctgttcc agattcagga gcacggatct
      661 ggatttgact tctccgactt cgactacagc acgcgcgccg ggccgaagaa cacggcccat
      721 ttcaaagaga gtggctctca tgaaatcctc ccattcagag agagaattca ttactgacaa
      781 ttctatagaa gaagtaacac tttcgagccc cagaaaccag gacctcatcc agttatcgct
      841 gatatagtcc accttatcca gaaatggcgg ggaagtaaca atcagatcca cgctcgaagt
      901 gcgcacagtc ttcagatcct gcgaattttc agagatatag cgattgcccc tggcagcctc
      961 gttgaaaact cccggcagag gaagggaaag atctcgtttc atcttgcgga gaattctttc
     1021 gcggatgctt ctgtattccg gcttttgccc aagtcgcgcg ttgttctttc tttgtgcgcc
     1081 aggcatgatg gatatttgcg ggaatgaata tacagaaaag aaaccatccg aatgtccgtg
     1141 aaggcgagac agcgcagtca cgcctatgta tcgcatggcg ggatcttcca tgttatccag
     1201 gagttcggac ttgaggcaca gtatttcgct taacgttctt ggatgaaaga acggagaaag
     1261 cctctccttc tcttccggag tgggtttcat tcttcgatcg agttgcagag catcaaggcg
     1321 cttttccagt attttcatgg cgggaacatt ctgtctcgac ctggccagga aaacggatac
     1381 ggggttcaaa tcgttatgga cagcgcgatg tcccagtaga ttcgcctgca gagttgtagt
     1441 gccgcgacca ccaaacggat ctaacaccac acctggaacc ttcaaatagc gattgatgaa
     1501 gaagtcaggt agctctggct taaatgagcc gcggtagctg atcgtatagt ggattgggtg
     1561 catctgtctc tggcgcgcag tccagagttc gccggaatgc accggcagag aattgatcgc
     1621 ttgcaaaaga atactccgaa tgctttttca tcggagttcc aggagcgtac cggtgagaaa
     1681 aacggctgtc gcagcgcccg cgcgcaaaaa atctgacgca tgcaccgaat cgtttacgtc
     1741 aagaaggaag actgcacctc ctgcaatcag tgcgcagaca gcctcccctc ctactttcgc
     1801 atggacgatc acgatacggc agaatcacac gttaagaatt ccagcatcaa tgcggcgccc
     1861 gtccccgagt ccgactggaa ggcagtgcaa acagaaatgg atgaatgccc gggcgaatgc
     1921 ataaaatgga aggaatgatt cgtccgggtc cttcattttg cgcgaaacgt tcgaatcata
     1981 aaattttttt aacttaagtt tcccaggttc accgattata ggttgtaagc ggatatccgc
     2041 caacatcagg ttcacggagg accgggtatg attatcaacc ataacatcag tgcgatctac
     2101 tcgcacagaa ccctcaaatt ccacgacatg gacgagtcca aggatattga gaaactatcg
     2161 agcggtatgc gaatcaatcg cgcaggcgat gatgcagcag gacttgccgt ttccgaaaag
     2221 atgcgcacgc agattcttgg cctgagacag gcagaacgca acgcggaaga cggaatttcg
     2281 ttcgttcaga caactgaagg ttttctgagc gaaactcaga acatgatcca gaggattcgt
     2341 gttctcgccg tccaatcagc caacggtatt tattcagatg aagaccgtca gcagatccag
     2401 gtggaagtat cacagctgat cgacgaaatc gacagaattg catcccaggc agaattcaac
     2461 aaaatgaaga tgctgacagg cgcgttcgct cgcttgaatc ctacagcttc tatgtggttt
     2521 cacattggac caaacatgca ccagcgcgag agagtgttca tcaacacgat gaccacacag
     2581 gctctgggcc ttcgcaaccc gaccgtactg acgttcattt cgatcagtac accggacaaa
     2641 gcgaacagcg tgattggact tgctgatgaa gcacttcgca aaatctccaa gcaaagagcg
     2701 gatcttggtg cttaccagaa ccgtctcgag catgcggcac gtggcctcat gaacgcttac
     2761 gagaacacgc aagcagctga atcccgcatc cgtgatacgg atatggcgga gcaaatgacg
     2821 agctatactc gttatcagat tcttgttcag gcagcaacag ccatggttgc acagtcgaac
     2881 atgaagagcc agaccgttct tcagcttctg aagtaacagt cacttcccga tcctgatgta
     2941 gactgccggc cttcgggccg gctattctat tttccattgt ttacgaatgg atcgaagatg
     3001 gccttcagac gaatgctgtc ttccctcttg atcgcgatag acagcgcgct tagattttcc
     3061 ttcgcatgaa gcgaatctcg aatgcctgta atggccgctc caatgcctgg aacagaaagc
     3121 acccaggcaa tcgcaagctg cgacaagttt acgttatacg acgaagcaat ggcacgagcc
     3181 tgatcgagag ctggttctat tctcgatatg tattcaggat ccatgatagt ggacccgcct
     3241 ctccgatccc cttccccgaa gacccggtct gatcgtatgg ctccagtcag caaaccctgg
     3301 gccagtggac tgtaggctag aatcggtatg gagtgtttta gcgtgtaggg ttttagaccc
     3361 gcgtcgatct ttctctgatt cagactgtat ctttcttgaa caa
//
LOCUS       DAORFF010000444         3399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_17793_length_3399_cov_3.327153, whole genome shotgun sequence.
ACCESSION   DAORFF010000444 DAORFF010000000
VERSION     DAORFF010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17793_length_3399_cov_3.327153"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PKK76_15315"
     CDS             complement(<1..511)
                     /locus_tag="PKK76_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69898.1"
                     /translation="MLDEYCDTLSDPALLEIALRLTELSLPVWDKHFSSHPEEERKLA
                     RAMNEATRVPGARTLDAHFPETALSMIEKCYADSKTGPSALEKLRGNPELIHLLCTSM
                     QPLTNQEWDEAFPRSVRLVFTSVWNILSWLVYTQYSSSDENHIYVAINQAADALLSEG
                     LFSVEQINAI"
     gene            complement(514..2691)
                     /locus_tag="PKK76_15320"
     CDS             complement(514..2691)
                     /locus_tag="PKK76_15320"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNL69899.1"
                     /translation="MPDLFSLMASAVYNGDAVRAKQLLDEGVPPNHIMPENLGHSFLT
                     FACLQGHLPVVQILIEAGAHINRKDSFRTSSGTDAGYYPLMAGCNARDHAPEIAKYLL
                     EHGAKVDLAHPSGRDGAIHFAAMRGDSAVVKLLLDEGVNVNQPGSYGSTPLTLALHMN
                     STSNLVRMLLNAGAEIDSSPNKPNPLFELIAGWLDEADLARLFIDHGVPTTDASGSAI
                     VQAASRGKTAILKTLLENKPLSSPDTTDAVLAAMKGQHFDAVEFLLRHGADPNAAERF
                     EGTLLRQATRAGNTKLVSLIRLKQKEKSETRNTGAMLAAAENGDLAMMRLLFESGLPI
                     DEVHDGEVWGSKKETPLMRAAARGRMEAVEYLMLHGASVDAVDEKGNTPLMYAAAAGQ
                     TEAIGYLLDHGASIDSTNHLNWNALMQACFTGKESAARLLLERGSRTDPIDLEKGATA
                     LSLARMSENTNLVKMLEERGVTDRLRRRRASNQQYAGIADCDICTYMAPRQELTHSYT
                     PEKTEDLLIIHEEGSVDYKQEITRMIKKCKECGTYYYHYHFIDTEDSIGGAGPICSHY
                     LIRLNLLWALDLFSKLEKTGELGELNQRYSQLIESFAILLRAENHIIPPYVLPTVVES
                     LNDFYVMKSSWNSIVTLLCHPTIAVLTLEDILRVFGENARDDTYPPFTPYRNFTKDAH
                     DKMRKLLRKNQKEFREHLNRIARSCDQKTSDFFLRTAQAYKIG"
     gene            2898..>3399
                     /locus_tag="PKK76_15325"
     CDS             2898..>3399
                     /locus_tag="PKK76_15325"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69900.1"
                     /translation="MIRIKVALLLLLVCLSSLASQPQSQAAYDRGIDALYNQKQPDRA
                     LEFFREAYRLDPNVWSRPFMIGYTLNAYLNQPSEALPFLNRAWALNDANDELPYKETI
                     QCLEKLQRWDAAIAKNQDAQKRLKAARKTIGSWYPENLAWLYWQKGDTTRALAIAPAG
                     SWVRSQL"
BASE COUNT          760 a          840 c          892 g          907 t
ORIGIN      
        1 gaatcgcatt gatctgttcc acagagaaga gtccttcgct caacaacgcg tccgctgctt
       61 gattgatggc cacatagata tggttttcat ctgatgagct gtactgagta tacacaagcc
      121 agctcagtat gttccagact gacgtaaaga caagtcgaac ggatcgcggg aatgcttcat
      181 cccactcctg gttcgtcaga ggttgcatgg aagtgcacaa aagatgaatg agttcagggt
      241 tgccgcgaag cttttcaagc gcggagggcc cggttttaga atcggcatag catttctcaa
      301 tcatagagag agctgtctct ggaaaatgtg catccagtgt tcttgcgccg ggcacgcggg
      361 tcgcttcgtt cattgctcga gccagttttc tctcttcttc cgggtgggaa gagaagtgtt
      421 tgtcccagac tggcaaagaa agttctgtca ggcgaagtgc gatctcaagt agcgcgggat
      481 cggaaagtgt gtcgcagtat tcgtccagca tttctagcct attttgtatg cttgtgcagt
      541 tctcagaaaa aagtcggagg tcttttgatc gcagcttcgc gcaattcgat tcaagtgctc
      601 tctgaactcc ttctgattct tcctcagcaa ttttcgcatc ttatcgtgag catctttcgt
      661 aaagtttctg taaggcgtga aaggtgggta agtgtcatcg cgcgcattct ctccgaaaac
      721 gcgcaagata tcttcaagag taagcacggc gatggttgga tggcaaagca aagtcacaat
      781 ggaattccag gaacttttca tgacgtagaa atcattcagc gattccacga ccgttggaag
      841 tacatagggt ggaatgatat ggttttctgc ccgcaacaga atggcgaacg attcaatcag
      901 ctgactgtat cgttgattca attcgcccag ttccccggtc ttttctaatt tcgaaaacaa
      961 atccagggcc cagagcaggt tgagtcgaat caggtagtgc gagcagatcg gtcccgcgcc
     1021 tcctatcgaa tcttctgtat caatgaagtg atagtgatag tagtaggtgc cgcattcttt
     1081 gcatttcttt atcattcgcg tgatttcttg tttgtagtcc acgcttcctt cttcgtgaat
     1141 gatgagcagg tcctctgttt tttccggagt gtaggaatga gtcaactcct ggcggggcgc
     1201 catgtaggtg caaatatcgc aatcagcaat gcccgcatat tgctgattcg aggctcttct
     1261 tcgccggagt ctatccgtca ctcctctttc ttcgagcatt ttcacaaggt tggtgttctc
     1321 tgacatcctt gccagggaaa gtgctgtagc ccctttttcc agatcgattg gatctgtcct
     1381 cgaaccgcgt tcgagcagca gtcttgcagc actctctttt ccggtgaagc acgcttgcat
     1441 gagtgcattc cagtttaaat ggttggttga gtcgatggaa gcgccgtgat cgagaagata
     1501 tcctatggct tctgtctggc ccgccgcggc cgcatacatg agcggtgtgt ttcctttttc
     1561 gtcgactgca tctacgcttg ctccgtgcaa cattagatac tctactgctt ccattcttcc
     1621 gcgcgcagcg gctctcatca gtggagtttc ttttttcgaa ccccagactt caccatcgtg
     1681 aacttcatcg atcggaagtc ccgattcgaa gagcaaacgc atcatcgcaa gatcgccgtt
     1741 ttctgccgcg gcaagcatag cgccggtatt tctggtttcc gatttttctt tttgcttgag
     1801 acgaatcaga gaaacaagtt ttgtatttcc cgctctggtg gcctgtctga ggagtgtgcc
     1861 ctcaaatcgc tcggctgcat tcggatcggc gccgtggcgc agaagaaact ccactgcatc
     1921 aaaatgctgc cccttcatcg ctgcgagcac agcatccgtg gtgtcgggtg atgacaatgg
     1981 tttgttttca agcaaagtct tcaggatggc agtcttgccg cgcgaggccg cttgaacgat
     2041 agcgctgccc gaagcgtctg tggtcggcac tccgtgatcg atgaacagac gggccaggtc
     2101 cgcttcatcc agccagcccg caatcaattc gaaaagtggg tttggtttat tgggagacga
     2161 atcgatctcc gcgcccgcgt ttagaagcat tcttacgagg ttggatgtcg aattcatgtg
     2221 caaggcaagc gtcagaggag tcgatccata gctacccggc tgattaacat ttacgccttc
     2281 gtctaacagc agtttcacga ctgccgagtc gccacgcatg gccgcaaaat gaatcgcgcc
     2341 gtctctacca gaaggatggg cgaggtcgac ttttgcacca tgttccagaa ggtatttggc
     2401 gatttctggt gcatgatcgc gcgcattgca tccggccatt agtggatagt atcccgcatc
     2461 ggtaccgctc gaagtgcgaa acgaatcttt tcggtttata tgggcgcccg cttcgatcag
     2521 tatctggact actggcaggt gtccctgtag acaggcgaat gttagaaagc tatgtccgag
     2581 gttttcgggc atgatgtgat tgggtggaac tccttcgtct aatagttgtt ttgcgcggac
     2641 cgcgtctcca ttgtacacgg cggacgccat gagcgagaac agatcaggca cagtgccgcg
     2701 tccggcaatg gtagagtgaa gtggccagaa gttgtccccg gtggctgatt gccggtatcc
     2761 tacgatcgca cgccttcatg atctcttctt tctacggggt gcttcgctcc ggtcgagcta
     2821 aaatgctcga aaatcgcggg cgtgaaattc atcgtttttg acttgcatgg tgacgcgctg
     2881 cgctctacaa acatcgcatg atcagaatca aggttgctct tctacttctt ttggtttgtc
     2941 tctcttcact cgcgtcgcag cctcagagtc aggcggccta tgacagagga atcgacgcac
     3001 tctacaacca gaaacagcca gacagagcgc tcgaattctt tagagaagcg tatcgcctgg
     3061 atccgaacgt ttggagcaga ccgttcatga ttggctatac tctaaacgcg tatctcaatc
     3121 agccgtcgga agctctgccg tttttgaaca gggcctgggc tctcaatgat gcaaatgatg
     3181 aacttcctta taaagagaca atccagtgtc tggagaaatt gcagaggtgg gatgctgcta
     3241 tcgcaaagaa tcaggacgcg caaaaaagac tgaaggcagc gcgaaagact atcggctcgt
     3301 ggtatcccga gaaccttgca tggctttact ggcagaaggg agatacaacg cgagcgcttg
     3361 caattgcacc ggcagggagc tgggttcgat cccaacttg
//
LOCUS       DAORFF010000445         3381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_17973_length_3381_cov_3.179495, whole genome shotgun sequence.
ACCESSION   DAORFF010000445 DAORFF010000000
VERSION     DAORFF010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17973_length_3381_cov_3.179495"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PKK76_15330"
     CDS             <1..349
                     /locus_tag="PKK76_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69901.1"
                     /translation="ESLGQVLAQDDHTITIGRRACEGECVAETNTSRKVIQKEDIVKT
                     VWLAGADAPEAWWNFKKFSARGATAKKMLAIAQENNVSCANCHVAHGDFRLTSEGKEY
                     LKTGKVIHTKPLH"
     gene            complement(346..2934)
                     /locus_tag="PKK76_15335"
     CDS             complement(346..2934)
                     /locus_tag="PKK76_15335"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNL69902.1"
                     /translation="MNLRREIILSLKQTRRSRGKFLFSAMAIVLGVAAISAVRSFSSS
                     LENAVSLESRKLMGADMRLEAGSSIPQNGMTAELLGAGARAADVIEFNTMLRVEKPKP
                     SVPLSTRLVRVRAIASEYPFFGRITSEPESGFARVREGRLPVVLVDSTLLPALGLVEG
                     DSVFLGKQKFRVAGVLRREPGSPGFSAGIGAPVYIHVSFAEKTGLLVTGSRIRYARYF
                     KLPDFFPVEEWKEKHWQEALNANITIYTYNEAASGVRRFMDNLTRFMTLAGLVILLLG
                     GLGIGLAMNVFVRSRMDDIAVMRALGATPGGTMRVFFILGFLTALVGSVLGYAIGFFG
                     ARAGADFGAAYLPVEVHVRFDALSALLSILPGILFTMAFVLFPVFEIRRISPLRVLRR
                     QAEDQTEDGPGLRGFVSKVIRLKYEIISAVVLASVITGVSAGQSESIAVGAFFTSGVA
                     LAVAVLYLLSLGLIRLSRLVMPWIGSYRIRQGIANLHRPGNQTSVMLTSAGVGALLIV
                     SLYTVQATLQREMRFDGGGRPNLYIMDVQPDQREGVEAVVHRYAASSQLVPMVSMRVK
                     AINGEKIDRSNIEKNANRRNWENSLRSYEYFASYRDHLNTSEKLARGSFWGTRRPENQ
                     EVSVDERWADRLGIRMGDLLEFDIGGAPFEATVTSMRNVDWAAMQINSILLFSPGAIE
                     EAPHIFVSSLSVPAEKDRFRFQEELVNRYPNISVIDVNQVVETIGGIAENVALVVRSI
                     AFSTLIAGLIILTGAIGAGRFARVRESALFKTIGARSRDIRVILSTEYALLGFLGALA
                     GSVLSQAVSWPLLYYLFDLRAAVPLDAIVAMSVSTGLISTLVGMLISRGVAASSPLEV
                     LREETV"
     gene            complement(2931..>3381)
                     /locus_tag="PKK76_15340"
     CDS             complement(2931..>3381)
                     /locus_tag="PKK76_15340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNL69903.1"
                     /translation="EIMGRADAKEKAEELLTRVGLAERMHHYPAQLSGGEMQRVAIAR
                     AFVNYPPILFADEPTGNLDSLNGDRIVELLLDLNQTYESTLVLITHNPELAGLSDREF
                     RLRDGKVVQVVAHRRGLRGKASSPNGRSGSRRPVQKRGTRSGSGVRK"
BASE COUNT          905 a          932 c          821 g          723 t
ORIGIN      
        1 ggaatccttg ggacaggttc tcgctcagga tgatcacacc atcaccatcg gtagaagagc
       61 gtgcgaaggc gaatgcgtgg cagaaacgaa tacctcacgc aaggtcattc aaaaagaaga
      121 catagtcaag acggtgtggc ttgcaggtgc ggatgctccc gaagcgtggt ggaacttcaa
      181 aaaattttct gcgcgcggag ctacggcaaa aaagatgctc gcgatcgcgc aggaaaacaa
      241 tgtatcgtgc gcgaactgcc acgtggcaca tggagacttc agactcacat cagagggaaa
      301 agaatatctg aagactggca aagtgatcca cactaagcct cttcattaaa ctgtttcctc
      361 acgaagcact tcaagtggac tgctcgccgc cacgccgcga ctgatcagca tacctaccag
      421 ggttgaaatc aatccggtag aaacactcat cgcaacgatc gcatccaggg gaacagccgc
      481 acgcaaatcg aacaaatagt agagcaaggg ccaggaaacc gcctgggaaa gaacagatcc
      541 ggccagcgct cccaggaatc caagaagggc atattctgta gataggatca cacggatgtc
      601 ccgacttctc gctccaatcg tcttgaacaa agcggactcc ctgacccttg caaatcgccc
      661 ggctccaatg gcaccggtca gaatgatcag acctgcgatc aatgtggaaa atgcaatcga
      721 gcgaaccaca agagccacgt tctcagcaat gccgcctatc gtttctacga cctgattcac
      781 atctatcact gaaatatttg gatagcgatt cacaagttct tcctgaaaac gaaacctgtc
      841 tttctcagct ggaacagaca gactggaaac gaaaatgtgc ggcgcttctt caatggcccc
      901 gggagaaaac aacaggatgg aattgatctg catggcggcc cagtctacat ttctcatgct
      961 cgtgacggtt gcctcaaagg gggcgccccc aatgtcaaat tcaagcaggt cccccatgcg
     1021 aatccccagt cgatcggccc atctctcatc cacagacact tcttgattct ccgggcgcct
     1081 ggttccccag aagcttccgc gagccagctt ctccgaggta ttcaaatgat ccctgtaaga
     1141 agcaaagtat tcgtaactac gaagactgtt ctcccagttg cgtctatttg catttttttc
     1201 aatgttggac ctgtctattt tctccccgtt gatcgccttt acgcgcatgg aaaccatggg
     1261 gacgagttgt gaggatgccg cataacggtg aaccacagcc tctactcctt cgcgctgatc
     1321 aggctgtaca tccatgatat agagatttgg tctgccgcct ccgtcaaaac gcatctctct
     1381 ctgcagcgtt gcttgcaccg tatataaaga aacaatcaag agtgcgccaa cgccagcgga
     1441 ggtaagcatg acgcttgtct ggtttccagg tcgatgtaga ttcgctatcc cctggcgaat
     1501 tctgtaggat ccaatccagg gcatgacgag gcgcgaaagg cgaatcaaac ctagagacag
     1561 taggtagaga acagccactg caagcgcgac accggaagta aagaaagctc ctacggcgat
     1621 cgactcagac tgacctgcgg atacgccagt aatgactgat gcgagcacca ctgcggatat
     1681 gatttcatat ttcaaacgta tgactttaga cacaaatccg cgaaggcctg gtccgtcctc
     1741 cgtctgatct tctgcctgcc gcctgagcac gcgaagaggg gaaattcgtc tgatctcaaa
     1801 aacgggaaac aacacaaatg ccatggtaaa aagaattcca ggcaaaatag aaagaagagc
     1861 agaaagagca tcaaagcgaa catgaacctc tacgggcaag taagctgcgc cgaaatccgc
     1921 acccgcacgc gcgccaaaaa aacctatcgc ataaccaagg acgcttccta caagcgcggt
     1981 taagaagcca agaataaaga aaacacgcat ggttccgccc ggagttgcac ccagagcgcg
     2041 catgacagcg atatcgtcca ttctgcttcg cacaaataca ttcatggcaa gaccaattcc
     2101 cagtccgccg agaagtagaa tcacaaggcc ggctaacgtc ataaatcgag tgagattgtc
     2161 catgaaacgc cgcacaccgg aggcggcttc gttgtacgtg taaattgtaa tgttcgcgtt
     2221 gagcgcctcc tgccagtgct tctctttcca ttcttccaca ggaaagaaat ctggaagttt
     2281 gaagtatcga gcgtatctga ttctgctccc tgttaccagg agaccggttt tttccgcaaa
     2341 gctcacgtgg atgtagaccg gtgcgccaat cccagcggag aacccaggac tacccggctc
     2401 acgtcgcaga acacctgcta cgcgaaactt ttgttttccc aggaaaacag aatctccttc
     2461 caccagtccc agggctggaa gaagagtgga atccaccaga accacgggca ggcgcccttc
     2521 acgaacacga gcaaaaccgc tttcaggttc gcttgtaatg cggccaaaga aagggtattc
     2581 gcttgcgatc gctcgaacgc ggaccaggcg tgtgctcagc ggaacagaag gctttggttt
     2641 ttcaacgcgc aacattgtgt tgaattctat tacgtccgca gcgcgagcgc cagcacccag
     2701 aagctcagct gtcattccat tttgcggaat gctcgacccg gcctcaagac gcatgtctgc
     2761 acccatgagt ttgcgcgatt caagacttac cgcattctca agcgaagaag agaaagatcg
     2821 aaccgcagag atggctgcga ctcccagaac gatcgccatg gcgctgaaca gaaacttgcc
     2881 acgcgaccgg cgcgtttgct ttagagataa aatgatttcg cggcggaggt tcatttgcgc
     2941 acgccgcttc ctgatctggt cccgcgtttt tgaacagggc gtctactccc acttctaccg
     3001 ttaggcgaac tagccttacc gcgcaaccct cgcctgtgcg ccacgacctg tactacttta
     3061 ccatctctga gtctgaactc tcggtccgaa agcccggcga gttcaggatt gtgcgtaatc
     3121 aaaaccaggg tagactcgta ggtttgattc aaatccagga ggagttctac aattcgatct
     3181 ccgttcaggc tgtccaggtt cccggttggt tcatctgcaa ataaaatggg agggtaattt
     3241 acgaaagctc tcgcaatggc aactctctgc atttcaccgc cggaaagctg cgctggataa
     3301 tgatgcattc tttctgcaag tcccacgcga gtcagaagtt cctccgcttt ttctttcgca
     3361 tccgcccgac ccatgatttc a
//
LOCUS       DAORFF010000446         3376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_18007_length_3376_cov_3.881662, whole genome shotgun sequence.
ACCESSION   DAORFF010000446 DAORFF010000000
VERSION     DAORFF010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18007_length_3376_cov_3.881662"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="PKK76_15345"
     CDS             complement(<1..1147)
                     /locus_tag="PKK76_15345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNL69904.1"
                     /translation="MVSPVRLVGLFLIIFGFFAAAGTALSRRPAPPSLQRAKVFYDAE
                     LPERGDHEILDFLAASGDPADAWCLALAEHAESHSNVSDAMLKQLNEPGQLRYKTLFA
                     FVANLNQVSTVQESWPLLRDAGCVLERLHRDAEAIALLKKAEQIHPDPDQRLDILHLV
                     HSLGWKTDFRALMADPLYYEEADPFLRMNYAQSRGEFVLAGYWLIRHQIESYQPGTMI
                     AAAGAGLCWLLLMLQLGRVQNWSWKYTSLVPFALILGGLSAYATVFEISIEQRFFVFE
                     RYSPHWLLNLIYAVLGIGLREETLKLLFSLPVILLARKAEPIVVLALASCTGLGFAIE
                     ENTQYYAGDWGAVFARFLTANFAHMMWTGMAGLAFTDALKGRTSWGEF"
     gene            1238..1723
                     /locus_tag="PKK76_15350"
     CDS             1238..1723
                     /locus_tag="PKK76_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HNL69905.1"
                     /translation="MTGIDYQVNPLWQFLRETYGFEKAFAFFGPYAGASIRVEIVDSN
                     TIVSSMDLVMTNTNYVGSHFGGSLYSMCDPFYMFILMENLGPDYIVWDKSASIDFIRP
                     GRGLVRARFHISKEEIEKIRDQVAKNRKMDWTADCEILDESGTVVARVHKVLYVRKNK
                     K"
     gene            complement(1730..2191)
                     /locus_tag="PKK76_15355"
     CDS             complement(1730..2191)
                     /locus_tag="PKK76_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNL69906.1"
                     /translation="MAIHILRETELIPISQAEAWDFFSRPENLKTITPPGIGFDIQGN
                     PPEKIYPGLMITYIVKPLFGIPLTWVTEISHVREGEYFVDEQRIGPYKIWHHEHHFAA
                     KGASTEVTDIVTYALPFGILGKIAHAIIVKPRLRAIFDFRREKIRSLFPSK"
     gene            complement(2231..3322)
                     /locus_tag="PKK76_15360"
     CDS             complement(2231..3322)
                     /locus_tag="PKK76_15360"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNL69907.1"
                     /translation="MIFSSDRPGGYFTYRPVTELKGGFLMGNTDLYVAFRKPDGTWST
                     PRNLGPYLNTPGAERTPFLYADGKTLYFSSDGYNGFGRSDVYKSVRLDDTWLHWSTPE
                     NLGPLVNGAGQDWGFRLTAASDRGYFSGELPGTVGGQDIFEITPLPPAIRPASIVMAV
                     RGTVRDDMSVPLESQIEWQDLATGQSLGIMSSKPVSGEFFIALPAGREYAYFARKPGY
                     FPVSQRVDLRNTQSYFEKTVDIKLVSIASAQAKNVEIVLNNIFFESGSATLNPRSFSE
                     LDRLAVILKQNPNLRVEIQGHTDDVGQPAANLELSANRARSVLQYLVQAGISRQRLDS
                     KGFGSTKPVAPNTTEEDRQKNRRVSFTLQ"
BASE COUNT          817 a          833 c          875 g          851 t
ORIGIN      
        1 agaattcgcc ccaggaagta cgtcctttga gagcgtccgt gaaggcaaga cccgccatgc
       61 cggtccacat catatgtgcg aaattcgcag tgagaaatct ggcaaacacg gctccccaat
      121 cccctgcata gtactgagtg ttttcttcga tggcaaagcc gagtcctgta caggaagcca
      181 gggccagaac tacgatgggt tctgccttac gggccagtaa tatcactggc aatgaaaaca
      241 gcagcttcag tgtttcttcg cgcagaccaa tgcccaggac cgcgtagatt agattgagca
      301 accagtgagg cgaataacgt tcgaatacga agaacctctg ttcaatgctg atttcaaaga
      361 ccgttgcata tgcactgagt ccgcccagga ttaaagcaaa cggaaccagg gatgtgtatt
      421 tccatgacca gttctgcacc cggcccagct gcagcatcaa cagcagccag catagaccgg
      481 cacccgcggc tgcgatcatc gtcccgggct ggtaggactc tatttgatga cgaatgagcc
      541 agtatccggc aaggacgaac tcacctctac tctgggcgta gttcatacgc aggaatggat
      601 ccgcctcttc gtagtacaga ggatctgcca tcagggcgcg aaagtctgtc ttccatccca
      661 ggctgtgaac aaggtgaagg atatcgagtc tctggtcggg atcagggtga atttgctctg
      721 cctttttgag cagcgcgatt gcttctgcat cccggtgtag tctctcaagc acgcatcccg
      781 catcccgtag caatggccag ctttcttgaa ctgtgctgac ttgatttaga ttggccacaa
      841 aggcgaataa cgttttgtat ctcagttgtc ccggttcgtt cagttgtttg agcatcgcat
      901 ccgaaacatt gctgtgcgat tctgcatgct ccgccagggc aagacaccag gcatctgctg
      961 gatctccgga tgctgccaaa aaatccagga tttcatggtc tccgcgctcg ggtagctcgg
     1021 catcataaaa cactttcgct ctctgcagac tgggaggagc cgggcgacgc gaaagggcgg
     1081 tgcctgcggc ggcaaaaaag ccaaagatga tcagaaatag accaaccagc cgaacgggag
     1141 aaaccacacg tttgcttctc agagatgcgg ggtgcggtca agtagaacct ggcttgcaat
     1201 tggattgcgc tcaccaggcg gaaagccagg aggggttatg actggaatag actatcaggt
     1261 gaatcctctc tggcaattcc tcagggagac gtacggattc gagaaagcat tcgcgttttt
     1321 tggcccttat gcaggcgcca gtatccgtgt tgagattgta gattccaata cgatcgtaag
     1381 cagcatggat ctggtgatga caaataccaa ttacgtaggt tctcattttg gcggatcgct
     1441 gtactccatg tgcgatcctt tctacatgtt cattctgatg gagaatctgg gaccggacta
     1501 tattgtatgg gacaagagtg ctagcattga ttttattcga ccgggtcgag gtctggtccg
     1561 tgcacgattt cacatttcga aagaggaaat tgaaaagata cgcgatcaag ttgcaaagaa
     1621 tcggaagatg gattggactg cagattgcga aattctagat gaatccggga cagttgtggc
     1681 tcgcgttcac aaagtactct acgtgcgaaa gaacaagaag tagccttttt tatttagaag
     1741 ggaaaaggct tctgatcttc tctctacgaa aatcaaagat ggcgcgaaga cgcggcttca
     1801 caatgatcgc gtgagctatc tttcctagaa ttccaaacgg cagggcatag gttacgatgt
     1861 ctgttacttc agtagacgct ccttttgccg cgaaatgatg ttcatgatgc cagatcttgt
     1921 agggtccgat tctttgctcg tccacgaaat attctccttc gcgaacatga gatatttccg
     1981 taacccaggt cagaggaatg ccgaataacg gttttacaat gtatgtgatc atcaagccgg
     2041 ggtagatttt ctccggagga ttcccctgaa tatcaaaacc gattccagga ggagtgattg
     2101 tcttgaggtt ttccgggcgt gagaaaaaat cccatgcttc tgcttgagat atagggatga
     2161 gttccgtttc gcgcagaatg tgaattgcca caggatacta tactggaatc ggacgaatcc
     2221 ggctactcgt tcattgcaga gtgaatgaaa cgcgtctgtt tttttgtctg tcttcttccg
     2281 tcgtgttagg cgccacgggc ttcgtggatc caaatccctt tgaatccagt ctttgcctcg
     2341 aaatgcctgc ctgaaccagg tattggagga cggatctggc tctgtttgca ctcagttcaa
     2401 gattggctgc gggttgtcca acgtcgtctg tatgtccctg gatttctacg cgaagattag
     2461 gattctgttt taggatgacg gccagtcgat ccagttccga aaagcttctc gggttgagtg
     2521 tggcacttcc ggattcgaag aaaatgttgt tcagcacgat ttcaacattc ttagcctgag
     2581 cagacgcaat cgaaacgagt tttatatcca cagtcttttc gaagtagctt tgcgtatttc
     2641 gcagatccac gcgctggctt accggaaaat atccaggttt gcgtgcgaag taggcgtact
     2701 ctcttcctgc gggcagtgcg ataaagaatt ctccgctcac aggtttgctc gacatgattc
     2761 caaggctctg gccggtggcg aggtcctgcc attcaatctg gctctccagc ggaacgctca
     2821 tgtcgtcgcg caccgtgcct cgaactgcca tcacaatgga cgccgggcga atcgccggag
     2881 gaagaggagt gatttcaaaa atgtcctggc ctcccacggt tcctggcaac tctccagaaa
     2941 aataaccgcg gtctgatgca gccgtgagtc tgaaacccca gtcctgccct gcgccattta
     3001 cgagaggtcc tagattttct ggcgtagacc aatgaagcca cgtatcatcc aatcttacag
     3061 atttgtatac atctgatctt ccgaagccgt tgtatccgtc tgacgaaaaa tagagcgtct
     3121 ttccatctgc gtataggaat ggcgtacgtt ccgctcccgg cgtatttaag taaggtccca
     3181 gatttcgcgg tgtgctccag gttccgtctg gtttgcggaa ggcgacatag agatccgtat
     3241 tccccatcaa gaatccgcct ttcaactcag tcaccggtct gtatgtaaaa tatccacccg
     3301 ggcgatcgct tgaaaaaatc aacccgatga cctacgccaa cgccaagggc tggctggtgc
     3361 tcggcgtgct gatgct
//
LOCUS       DAORFF010000447         3362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_18142_length_3362_cov_3.146659, whole genome shotgun sequence.
ACCESSION   DAORFF010000447 DAORFF010000000
VERSION     DAORFF010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18142_length_3362_cov_3.146659"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PKK76_15365"
     CDS             complement(<1..890)
                     /locus_tag="PKK76_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNL69908.1"
                     /translation="MKTELKAKIEEELLANLNPVQKQAVLHNEGPLLILAGAGSGKTR
                     VITHRIAYLCRVLNVPPYRVCAVTFTNKAAGEMHQRLYGLIGPMADSVLVRTFHSLGL
                     YILRRNAPKLGLKSGFSIYDQNDQEALIKRVIKDLDITSSWLTPRAAAERINRARDQM
                     VSPEKFEQTADRYEEDVIPIYREYMIRLRKNNALDFGDLIYETVRMFEREPEVLADYQ
                     SMWRYFMIDEYQDTNTAQYLIVKYLAEKTRNVCVVGDDDQSIYSWRGADIRNILNFEK
                     DYPEANVIRLEHNYRSTKEIL"
     gene            complement(887..2008)
                     /locus_tag="PKK76_15370"
     CDS             complement(887..2008)
                     /locus_tag="PKK76_15370"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69909.1"
                     /translation="MDAGIVAELSRQSVIALGRTWPNPSVACVIQSKGQIFSGATEIP
                     GRRHAEIVALDLFDQSLPRDADGDLYVTLEPCSKFGRTPPCTKRLANYPNLRLHIGNL
                     DPSLSGEGLARLRESGLQIVDDSNGMGREFLEGFIGRTLGQGPRFHLKVASCDGIMGS
                     KEKRIMVSSEDGIAFGQMLRAKLDAVLVGPRTIFVDRPGLEARTRSPLRMKRTSGQDP
                     FVDAIIANDELAATNSNQPLRVFVLGRPFPGAETWFEKQKALTETTGREPLFLSVEPW
                     PGRWTHALPPLSHPSFMIELRDVLADRGLGEVLVEGGAGLFHAIMPGLGSRDRLYHLE
                     RSGTLEAGPDSVRVPSALLQARPMASYHAGEEKLGVYHF"
     gene            complement(2013..2948)
                     /gene="secF"
                     /locus_tag="PKK76_15375"
     CDS             complement(2013..2948)
                     /gene="secF"
                     /locus_tag="PKK76_15375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNL69910.1"
                     /translation="MKFSRFRAVFILGSLALGAVLFYITFFVYGGFQSSITFNGGIRF
                     SLVLPAGLGKADLEKAALKAGVDNPQIKLSGFRTNQYDLEIGPKTKEKLEKELAALKP
                     SEKPKGDGGVTAELESRILGNLGLTPRSVVSRETMAASYGDELWRISLWSLFWCIIII
                     GIYLSFRFDFAFALGASLSLVHDVILTIGFIGVARIEPSIPVVAAVLTIVGYSINDTI
                     VIFDRIRENSKDRAQMSLRTTMDEAMTQTLSRTLVTSILTMISVLALLLGGADSLRDF
                     ALVLIFGIFMGTYSSIFVAAHSVQYYEEFVARIRR"
     gene            complement(2945..>3362)
                     /locus_tag="PKK76_15380"
     CDS             complement(2945..>3362)
                     /locus_tag="PKK76_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNL69911.1"
                     /translation="MTAVLSFLEFTLTLPGFAGLILTVGMAVDANVIIYEKMKEDIRA
                     GKTASLAIEGGFRASFWTIFDSHVTTLISSIILFKIGDGPIKGFAIVLFFGLITSLFT
                     SLYVSHLIFDWFSLLFRPERLSIGWGYSRYAQGGKK"
BASE COUNT          873 a          878 c          816 g          795 t
ORIGIN      
        1 aggatttcct tcgtcgaacg gtaattgtgt tcaaggcgga tgacgttcgc ctcgggatag
       61 tctttttcga agttgaggat attgcggatg tcggcgccgc gccatgaata gatcgactga
      121 tcatcgtcac cgacgacgca gacgtttcgg gtcttctcgg ccagatactt gacgatcagg
      181 tactgcgccg tgttcgtgtc ctgatactcg tcgatcatga aatatcgcca catgctctgg
      241 tagtctgcga gcacttctgg ctctctttcg aacatgcgca ctgtctcgta gatcaaatcg
      301 ccaaaatcaa gagcattgtt ttttctcaga cggatcatgt actcgcgata aatcggaatc
      361 acgtcctctt catacctgtc cgccgtttgc tcaaactttt caggagaaac catctgatcg
      421 cgtgcgcgat tgatcctttc tgcagcagcg cgcggagtga gccacgacga agtgatgtct
      481 agatccttga tgactctctt gatcaatgct tcctgatcat tctgatcata gatagaaaag
      541 cctgacttca gtcctagttt aggcgcgttc cttcgcagaa tgtaaagccc aagagagtga
      601 aacgtgcgaa ccagtacact gtccgccatg ggaccaatca aaccgtagag cctctggtgc
      661 atttctcccg cagctttgtt tgtaaaggta acggcgcaca ctctgtacgg cggaacattc
      721 agtacgcgac acagataggc gattctgtga gtgatgactc gcgtctttcc ggatccggcg
      781 ccggctagaa tcagaagagg accttcattg tgcaacaccg cctgcttctg aaccggattc
      841 agattggcca aaagctcctc ttctatcttt gcctttaatt ctgttttcaa aaatgataca
      901 ctccgagctt ttcttcgccc gcatgataac tggccatggg ccttgcctga agaagcgcag
      961 acggaacgcg gactgagtcc ggtcctgcct ccagagtccc tgacctttcc agatgataca
     1021 aacgatcccg actgcctaga ccaggcatga tcgcgtgaaa caatccagca ccgccttcca
     1081 ccagaacttc acccagaccg cgatctgcga gcacatcgcg aagttctatc atgaaagatg
     1141 ggtgagacag agggggaaga gcgtgcgtcc acctgcccgg ccatggttct acggacaaaa
     1201 agagaggttc tctcccggtt gtttctgtaa gtgccttctg tttttcaaac caggtctctg
     1261 ctcctggaaa aggacgaccc agaacaaata cgcgaagcgg ctgattggaa ttcgtagccg
     1321 ccagttcgtc attggcaatg attgcgtcta caaacggatc ctgcccggag gttcgtttca
     1381 ttctcaaagg agatcttgtt cgcgcctcaa gccctgggcg atcgacaaaa atagtgcgcg
     1441 gaccaaccag aacggcgtcc agcttcgcac gaagcatctg accaaacgca attccatcct
     1501 cggaggacac catgattctc ttttctttgc ttcccatgat cccatcgcaa ctggcgacct
     1561 tgagatgaaa ccgcggtccc tgcccgagag tgcgaccaat gaatccttcg aggaattcgc
     1621 gacccattcc gttagaatca tctacgattt gcagacctga ttcgcggaga cgagcaaggc
     1681 cctctccgct caaactcgga tctagattcc caatgtgaag tctcaggttt ggatagtttg
     1741 ccagtcgttt cgtgcacgga ggagtgcgcc caaatttcga gcatggttcg agagtcacat
     1801 acagatctcc atctgcatcg cgcgggagac tttgatcaaa tagatcgagg gcaacaattt
     1861 ccgcgtgccg acgccctgga atttctgttg cgccggaaaa aatttgtcct tttgactgaa
     1921 tgacacaggc gacggacgga ttgggccagg tgcggcccag agcgatcaca gattgccggc
     1981 tcagctctgc aactatgccg gcatccactt ttctaccgcc gaatacgagc gacgaattct
     2041 tcgtagtact gaacgctgtg cgcggccacg aaaatcgaag aatacgttcc cataaaaata
     2101 ccaaagatca gaacaagagc aaaatccctg agactgtctg cgccaccgag tagaagtgcc
     2161 agaacagaga tcatagtcag aatggaagta accagagttc gagagagagt ctgagtcatt
     2221 gcctcgtcca tggttgtgcg aagagacatc tgcgctctgt cttttgagtt ctcgcggatt
     2281 ctatcgaaga tcacaatcgt atcattgatc gagtaaccta cgatggtaag aaccgcggca
     2341 actactggaa tgcttggctc aattcttgcc actccaatga atccaatggt tagaatcacg
     2401 tcgtgtacca gagagagaga ggcacccagg gcaaatgcaa agtcaaatcg aaagctcaaa
     2461 tagatcccga tgatgatgat acaccagaac agactccaga gggagatcct ccagagttcg
     2521 tcaccgtagc tggccgccat ggtttcgcgc gacactacac tgcgcggagt gagacccagg
     2581 tttccaagaa ttctgctttc gagttcagcg gtcactccgc cgtctccctt tggtttctcc
     2641 gaaggtttga gggcagcgag ttccttttcc agtttttctt ttgtttttgg tccaatctcc
     2701 agatcatact ggtttgtgcg aaagccggac aacttgatct gaggattgtc cactcctgct
     2761 ttcaacgcag ctttttcaag atccgctttt ccaagaccgg ccggtaaaac cagactgaaa
     2821 cgaatgccac cgttgaaagt gatcgacgat tgaaaaccgc cgtacacgaa aaacgtaatg
     2881 taaaacagaa ccgcaccgag tgcgagagag cctaagatga agacggcacg aaagcgtgaa
     2941 aatttcactt cttgcctcct tgcgcgtagc gagaatatcc ccatccaata gaaaggcgct
     3001 ccggccggaa aagcagactg aaccagtcaa agatgagatg tgatacgtat agagacgtaa
     3061 acagagacgt gatcagacca aagaacagca cgatggcgaa gcctttgatt ggcccatctc
     3121 caatcttaaa tagaatgatg gaagaaatca atgtagttac gtgcgagtca aagatcgtcc
     3181 agaaagaagc tcgaaagcca ccttcaatgg caagagaggc tgttttgcct gctcgtatgt
     3241 cttccttcat tttttcgtag atgatcacgt ttgcatccac agccatacct acagtcagaa
     3301 tcaacccggc aaaaccaggt agagtgagag taaattcaag gaaactcaat acggccgtca
     3361 tg
//
LOCUS       DAORFF010000448         3334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_18400_length_3334_cov_2.842635, whole genome shotgun sequence.
ACCESSION   DAORFF010000448 DAORFF010000000
VERSION     DAORFF010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18400_length_3334_cov_2.842635"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PKK76_15385"
     CDS             <1..852
                     /locus_tag="PKK76_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69912.1"
                     /translation="DIKHDDLIAGTETTPGDSIQELPKIPERDDITIKNMAPVQKSIY
                     DGTATKLLSAGGGGLFVLGYLLYWLFGLGKDPYKKYKLFETLGFPFHYGAALAFGGAS
                     TAFEYGKTETFWSGIPVLLVASGLIAWLRGRKLKALRDEPRVCECGKTMRRLSEEEDD
                     SYLQRGNVVEEKIRSMDYDVWVCECGKHRIEAYSGDSPADKCEKCGFKTYQVASTTTI
                     RAATTSSEGLREIVYLCANCKHTETVRETIPKVSPPSSSSGSSSSSSGGSWGGGSSGG
                     GGAGSSY"
     gene            complement(846..2927)
                     /locus_tag="PKK76_15390"
     CDS             complement(846..2927)
                     /locus_tag="PKK76_15390"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL69913.1"
                     /translation="MILGLLVCKKVEPPHAVQGEMDLSTHDWSSQGPVSLQGMWRFYW
                     KEFRDPAIPLSGSSFIQAPGTWNEFDSGSGKAGGAGYATYALRILSKPDADLALRIQY
                     FDSSYRLYANGTLLAQAGVAGTTAENSIARRLPTTVRLMPHNGSIDLVIHVSNFAHRN
                     GGFPRELILGSPADIELIRTHKIMLDLFMAGAIIMMGLYHLALFFLRRSDYSPLYFGL
                     FCILIALRTDLVGERTIWGLTGSFGSFALSHRIEYATFYLSVPLFFAYTRSLFPDSYF
                     RKDILLTAAAIFGSATLLTFFVESYIYTQWLWIFQAALGFIGIYILVGVCKMVIVRRR
                     GARVFVGTFVLLFAAAINDVLYSRRLIESGFFIALGLFVFIFTQAFLLSRLYAESFRR
                     VEELAIDLTNSERRYRHLVEDSGEIILSLDSSGKILTSNKTSSRILGYSDISGRTLAS
                     LVFEQGQSNVFLARHVMEERLASLIQTGRADEFPAHFKTAQGDLCELNVRLQRVNLEG
                     GMTILANLAQKPLDLLARYCLEDHRHYVLFNSFHMGELLNQSLTAGLEARVSEEEQML
                     VRLALREMMINAIEHGNLGITFEEKTKATASGTLAELVSERLKNPYYSARKLHVRVDS
                     SEEKIVFSIRDEGEGFDHRAMLERARAQSDDSGLYHGRGLRFALSFFDSVEYNAPGNE
                     VRLTRLFSENQ"
     gene            complement(3034..>3334)
                     /locus_tag="PKK76_15395"
     CDS             complement(3034..>3334)
                     /locus_tag="PKK76_15395"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNL69914.1"
                     /translation="PTFHKDAGKICYGYQIHVTGPYHSLLHSLQLIRWIRSECHEFRW
                     NEGPYEFRSDRPAIELLAGHRVLLSYLNGETDLPHAEAAIQAGEHEWKNRIRSLS"
BASE COUNT          858 a          817 c          853 g          806 t
ORIGIN      
        1 gacatcaagc acgatgattt gatcgcggga acagaaacca ctcccggaga cagcattcag
       61 gaactcccga agattcctga aagagatgat atcacaatca agaacatggc gcccgtacag
      121 aaatccatat acgatggtac agccaccaaa ctcctttccg ccggtggcgg aggattgttt
      181 gtgctgggtt atctactcta ctggctcttt ggactcggaa aagatccata taaaaaatac
      241 aagttgtttg aaacactcgg ctttccgttc cactatggag ctgctcttgc ctttggtggc
      301 gctagcactg cgtttgagta tggaaagaca gaaacgttct ggtccggaat tccagtgctc
      361 ctggttgcat ccggtctgat tgcctggttg cgcggcagga agctgaaagc gctgcgcgac
      421 gaaccgcgag tatgcgaatg cggcaaaacc atgcgtcgtc tatctgaaga agaagacgat
      481 agctatttgc agcgcggcaa cgtggtagaa gaaaagattc ggtccatgga ttatgatgtg
      541 tgggtctgcg aatgcgggaa gcacagaata gaggcctata gcggggatag ccctgcggac
      601 aaatgcgaaa aatgcggttt caaaacatac caggtggctt ccactacgac cattcgcgcg
      661 gccacaactt cttcggaagg tttgcgcgaa atcgtttatc tttgtgccaa ttgcaaacat
      721 acagagactg tgcgcgaaac cattccaaag gtttctcctc catcgagctc cagtgggagc
      781 tctagctcca gttccggcgg gtcctggggc ggcggaagct ctggtggcgg tggagcggga
      841 tccagttact gattctcaga aaacagtctg gtcaaacgca cttcgttgcc cggcgcgttg
      901 tactccacgc tgtcaaagaa gctgagcgcg aagcgcaggc cgcgtccgtg atacaatccg
      961 gagtcgtcac tttgcgccct ggctcgttcg agcatggcgc ggtgatcaaa tccttctcct
     1021 tcgtctcgaa tggagaatac gatcttctct tcacttgagt ccacgcgcac gtgaagtttt
     1081 ctggcggagt agtacggatt ttttaaacgt tcggatacaa gttccgcgag tgttccgctt
     1141 gcagttgctt ttgttttttc ttcgaatgta attccaagat ttccgtgttc gattgcattg
     1201 atcatcattt cgcgaagagc cagacgcacg agcatctgct cctcttctga aacgcgcgcc
     1261 tctaacccgg cagtcagtga ctggttgagc aattctccca tgtgaaaact attaaaaagt
     1321 acatagtgtc tgtgatcttc gaggcaataa cgcgcgagca gatccagagg tttttgtgcg
     1381 agatttgcca gaatcgtcat ccctccttcc aggttcactc tttgcaggcg cacattgagt
     1441 tcgcagaggt ctccctgagc tgttttgaag tgagctggaa actcatctgc ccttcctgtt
     1501 tggatgaggc tcgccagcct ttcctccatc acatgccggg caagaaacac attgctctgg
     1561 ccctgttcaa ataccaggct tgcaagagtg cgaccggaaa tgtcagagta tcccagaatc
     1621 ctggaagatg tcttgttaga tgtgagaatc tttcctgacg aatccaggga caagatgatt
     1681 tcccctgagt cctccaccag atgcctgtat cgtctttctg aattggtgag gtcaatggcc
     1741 agttcttcta ctcttcggaa tgattcagca tagagacgcg aaagcaggaa tgcctgagta
     1801 aagataaata cgaaaagacc aagagcaata aagaatccgc tttctatgag tctcctactg
     1861 tagagtacat cgttgatcgc tgctgcgaat aacaacacaa aggtgcccac gaacactctt
     1921 gcgcctcttc tgcgcacgat gaccattttg catactccga ccagaatgta gatgccaatg
     1981 aatccgagtg ctgcctggaa gatccagagc cattgagtgt atatgtagga ttctacgaag
     2041 aatgtgagga gagtcgcact tccgaatatg gccgcagccg taagaagtat gtctttcctg
     2101 aaatacgaat ccgggaaaag acttcttgta tacgcaaaga acagcgggac ggacaaatag
     2161 aaagtagcat attctattct atggctgagc gcaaaagatc caaatgaacc cgtcaagccc
     2221 cagatggttc tttcccccac cagatccgtg cgcagtgcta tgagaatgca aaaaaggcca
     2281 aagtagagag gagaatagtc gctgcgcctt aaaaagaaaa gagcaagatg gtagagaccc
     2341 atcatgatta tggcacctgc catgaataga tccagcatga tcttgtgagt tcggatgagt
     2401 tcaatatctg ccggactccc caggatcagt tcacgcggaa agcctccgtt tctatgtgcg
     2461 aaattggaaa cgtgaatcac cagatcaatt gaaccgttat gcggcatcaa tcgcactgtg
     2521 gtagggagtc tccgggctat gctgttttct gctgtcgttc cagcgacacc tgcttgcgca
     2581 agcaacgtgc cattcgcata cagcctgtaa ctcgaatcga agtattgaat gcgaagcgcg
     2641 aggtccgcat ccggtttgga taaaattcgc agcgcgtagg tggcataacc ggcaccgccc
     2701 gcttttccag aacccgagtc gaattcattc cacgtgccgg gggcctgaat gaaactggaa
     2761 ccgctgagag gaattgcagg atcacgaaac tctttccagt agaatctcca cattccctgg
     2821 agggaaaccg gaccctgcga actccagtca tgagtgctca gatccatttc gccctgaact
     2881 gcatggggtg gttctacttt cttgcaaacg aggaggccca gaatcactgg aatgatccag
     2941 atgaaccggc atcttagcat atccgagagt aggtgattga tccagaaagg tcaattcaat
     3001 tcagattgcc tgcgacaatg ctcgtccagg gtttcacgat aaagaacgaa ttcgattttt
     3061 ccattcatgt tcccctgcct gaatggctgc ttctgcatgc gggagatctg tttcaccatt
     3121 gagataggac aatagaacgc ggtgacccgc tagaagttca atggcgggtc tatccgatcg
     3181 aaattcgtag ggaccttcgt tccagcggaa ctcatggcac tcgctgcgaa tccagcgaat
     3241 gagctgcaaa gagtgaagaa gagagtgata cggccctgtt acgtgaatct ggtaaccgta
     3301 gcagatcttg cccgcatctt tgtgaaacgt gggg
//
LOCUS       DAORFF010000449         3329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_18447_length_3329_cov_3.041539, whole genome shotgun sequence.
ACCESSION   DAORFF010000449 DAORFF010000000
VERSION     DAORFF010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18447_length_3329_cov_3.041539"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PKK76_15400"
     CDS             <1..381
                     /locus_tag="PKK76_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004494936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNL69915.1"
                     /translation="PITLPPGAINNTFQVSENGEATVRIGDDQRPTRIGQIDLYRFVN
                     PAGLQAIGKNLFKETIASGQEIQGTPGMDGMGGLMQGFVEMSNVKLVEEMVNMIVAQR
                     AYESNSKSIITSDSMLGTAIGMKR"
     gene            378..>838
                     /locus_tag="PKK76_15405"
     CDS             378..>838
                     /locus_tag="PKK76_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69916.1"
                     /translation="MIRFLILVLVCSSDLFAFKLFLEPVVRTQGGPVRLSDVSRSEGD
                     SPKDKVILGSIREAYFLSRDEIQRAAGTDCEAVYGPGVWVVPLTKTQDAEEAVESMKG
                     AIRRLSGGEEFLKKHTIKVSAGLKMPEQGASAVYRLPPQANKLAAGRMIVAV"
     assembly_gap    839..858
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <859..1315
                     /gene="flgA"
                     /locus_tag="PKK76_15410"
     CDS             <859..1315
                     /gene="flgA"
                     /locus_tag="PKK76_15410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNL69917.1"
                     /translation="IVPDNGKDRTLLRQQVELVIYRQVKVAVATHTLDQGDVIAHGDF
                     KMETRELDSDVEKFAPENLVGQRLIDRVDAGKPIYASQLRKTSTVRRGQSITLIHQTP
                     GIVVRCKSTSLTDAEPGGRIKVRILLPSGKKTDIKEADVVDENTAVLAR"
     gene            1278..1970
                     /locus_tag="PKK76_15415"
     CDS             1278..1970
                     /locus_tag="PKK76_15415"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNL69918.1"
                     /translation="MLSTKTQRFWRVSALAYSSLLSFYVIGGALIARDLWVDHNPYSP
                     SGRIVVGSILKLSIDEPMTVEYEFQGANSEEAKVKMTPDKSLTEFLPPANVDKNTQNK
                     QDSRARSRARVKFRMGVQVTRVTETGIEFTGNKMVAPEEGRTRFQVQVQGQVNREDIQ
                     TDRTIKSEDVSELQVLMRGGPVPQNKNLPMKQTPGTTTQGPGLSAQPTAEEKEQMLLD
                     YLNRIFGESRDF"
     gene            1971..3068
                     /locus_tag="PKK76_15420"
     CDS             1971..3068
                     /locus_tag="PKK76_15420"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNL69919.1"
                     /translation="MSLRFIGVLSILFTAGTLFAQAKKDDGVRIKDLARIQGVRSNLL
                     LGYGVVVGLPGTGDSKSKLGEESVRNLLGSLGQRIDSGSLQARNVAAVLVTAEVPAFA
                     NRGDKLNATVSSIGDAKSLEGGVLIQTPMQAGDGNMYAVAQGVVTLGGQTGNHQQKGR
                     TVGTVMSGVNVEKDIVSSYVFEQIQDKNEKGEKIDPPRRIQKIRITLNDFDFQTARDA
                     RDKIEKKFPESRPMIDNGSISVEIPAGKDVASFIAEVEDIRVSPRLKARIVVNERTGA
                     IVMGGDVRVEPVAVSRGGMDLIVSGNPYPNGVQLTRQDEKKPTMQPFSGASVQEIMDA
                     LNAMGASTRDIVAILEALRDSGALHAELIVQ"
     gene            3080..>3329
                     /locus_tag="PKK76_15425"
     CDS             3080..>3329
                     /locus_tag="PKK76_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69920.1"
                     /translation="MDLSTQSINQYGLQSAQDSRGADSIRLLKQRDEKRSTFADMVNE
                     HARPIIPKSKEKLMEEVRSNPENKKLYEAAQDFQAIFLG"
BASE COUNT          905 a          775 c          930 g          699 t
ORIGIN      
        1 ccgattacgt tgccaccagg tgccatcaat aatacgtttc aagtatctga aaatggagag
       61 gcgactgtgc gcattggaga cgatcaaagg ccaacgcgca taggtcagat tgatctatat
      121 cgttttgtaa acccggccgg tcttcaggcg atcggaaaga atctatttaa agagacgatc
      181 gcaagcggac aggaaatcca gggaactcct ggcatggatg gaatgggcgg attgatgcag
      241 gggtttgtgg aaatgtcgaa cgtgaagctg gtagaagaga tggttaacat gatcgtcgct
      301 cagcgcgcgt atgaatccaa ctccaagtcc atcatcacgt ctgacagcat gcttggaact
      361 gccattggca tgaagcgatg attcgatttc taatcctggt tctggtttgc agttctgatt
      421 tattcgcatt caaactcttt ctagaaccag tggtccgcac tcagggcgga ccggtgcgac
      481 tgtcagatgt cagccggagc gaaggggatt ctccgaaaga taaagtaatt ttaggatcta
      541 tcagagaagc atactttctt tccagggacg aaatccagag agcggcaggc acagactgcg
      601 aagcggtcta cggacccggt gtctgggtag ttcccctcac aaaaacgcaa gatgcagaag
      661 aagcagtcga gtccatgaag ggcgcgattc gcagactcag cggcggcgaa gaattcttga
      721 aaaaacatac aatcaaggtg agtgccgggc tcaagatgcc ggagcagggc gccagtgcag
      781 tgtatcgctt accacctcag gcaaacaaac tggccgcagg acgcatgatt gtggcggtnn
      841 nnnnnnnnnn nnnnnnnnga ttgttccaga caacgggaag gatcggactc tactcaggca
      901 gcaggtagag ctggtgatct atagacaggt gaaggtggcc gtggccaccc atactctgga
      961 tcagggagat gtcattgccc atggcgactt taagatggag acgcgtgagc tggactcaga
     1021 cgtggagaaa ttcgcaccag agaacttggt cgggcaaagg ctgatcgatc gggtagacgc
     1081 cggaaagcca atttatgcgt cccagcttcg taaaacatcc acagttcgga gggggcaaag
     1141 catcactctc attcaccaga cccccggaat cgtggttcga tgtaaatcca cgtccctcac
     1201 ggatgcagag ccaggtggcc gcatcaaggt tcgaattcta ctgccttcgg gcaaaaagac
     1261 cgatataaaa gaagcggatg ttgtcgacga aaacacagcg gttctggcgc gttagcgctc
     1321 ttgcgtattc ttctcttctt tcattttatg tgattggcgg ggctttgatt gcccgcgacc
     1381 tgtgggtgga ccacaatcca tacagtccat ccggacgaat tgtagtcggc tctattttga
     1441 aattgtccat tgacgaaccc atgacagtgg agtacgagtt tcagggcgcg aattcagaag
     1501 aagcaaaagt aaagatgact ccagataagt ctctgactga atttcttcct ccagcaaacg
     1561 tagacaagaa cacccagaac aaacaggact caagagcacg cagtcgcgca agagtgaaat
     1621 ttagaatggg cgtccaggtc actcgtgtca cagaaaccgg gatagaattt acagggaaca
     1681 agatggtggc accggaagaa gggcgcactc gttttcaggt gcaggtccag ggacaggtga
     1741 accgcgaaga catccagacg gatcggacca tcaaatcaga agatgtgtct gaactccagg
     1801 tgttgatgcg cggcggtccg gttcctcaga acaagaacct gcccatgaag caaactcctg
     1861 gcacaaccac acagggcccg ggactctccg cacaacctac tgcggaagaa aaagagcaaa
     1921 tgctcctcga ttatttgaac cgaatcttcg gcgagtcgag ggatttctaa atgagcctgc
     1981 gattcattgg ggtattgtct attctgttta ctgccgggac actctttgct caggcgaaga
     2041 aagacgatgg tgtgcggatc aaggatcttg cacgaattca gggcgtgcgg tcgaatctac
     2101 ttcttggata cggagttgta gtaggactgc ctggtacagg cgattctaaa agcaagcttg
     2161 gcgaagaatc tgtgcgaaac ctcctgggca gtctggggca gagaatagat tcaggcagcc
     2221 tccaggctcg gaacgtggct gcggttctag tcactgcaga agtgccggcg ttcgcaaacc
     2281 gtggagacaa gctcaacgct acggtttcgt cgattggaga tgcgaagtct ctcgaaggcg
     2341 gcgtgctgat tcaaactccc atgcaggccg gcgacggaaa tatgtatgct gtggctcaag
     2401 gagttgttac tctcggtggc cagacaggaa atcaccagca gaagggccgc acagtgggca
     2461 cggtgatgag tggcgtaaat gttgagaaag acatagtatc gagttacgtg tttgaacaaa
     2521 ttcaagataa gaacgagaaa ggcgagaaga tcgatccacc tcgtagaatt caaaaaattc
     2581 gcattactct caacgacttt gacttccaga ccgcgcgcga tgcgcgcgac aaaatagaaa
     2641 agaaattccc tgagtccaga cccatgatcg acaacgggtc gatctctgtg gagattcctg
     2701 cgggcaagga cgtggcttct ttcatcgcgg aagtggaaga cattcgcgtt tctcccagac
     2761 tcaaggcgcg catcgtggtc aatgaaagga caggtgccat tgtaatgggt ggagatgtgc
     2821 gcgtggagcc cgtggccgtg agccgtggtg gaatggatct cattgtttct ggcaacccgt
     2881 atccgaatgg agtccagctc accagacagg acgaaaaaaa gccaaccatg cagccatttt
     2941 cgggagcttc cgtgcaagag atcatggatg cactcaacgc aatgggtgca agcacgagag
     3001 acattgtagc catattagaa gctctccgcg attcaggcgc actccatgcg gagctgatcg
     3061 tccaataagg aagaatgaca tggacttaag cacacaatcg atcaatcagt atggactcca
     3121 gagcgcgcag gattcgcggg gtgcagacag cattcgcctc ttgaagcaga gagatgaaaa
     3181 gcgatccacc ttcgcagaca tggtaaacga gcacgcaagg ccaatcattc cgaagtcaaa
     3241 agaaaagctt atggaagagg ttcgctccaa tccagagaat aagaagctct acgaagcggc
     3301 tcaggacttt caggcgattt ttcttggtc
//
LOCUS       DAORFF010000450         3323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_18504_length_3323_cov_3.921359, whole genome shotgun sequence.
ACCESSION   DAORFF010000450 DAORFF010000000
VERSION     DAORFF010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18504_length_3323_cov_3.921359"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..569)
                     /locus_tag="PKK76_15430"
     CDS             complement(15..569)
                     /locus_tag="PKK76_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016758546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNL69921.1"
                     /translation="MRKLIFGINITADGHCSHTDGIADEELHEYFTELLRATDTILYG
                     RVTYQLMVPFWPDVAKNQSMSRTSNEFARVFDSLDKIVFSRTLKQAEGNARIVRENLE
                     GEVIALKQKPGKPICVGSLSIASQLSRAGLIDEYHFVVQPVVAGKGPRLFDTIKQDQS
                     IPLELLESKRFAQGQMALHYRKRT"
     gene            complement(588..1109)
                     /locus_tag="PKK76_15435"
     CDS             complement(588..1109)
                     /locus_tag="PKK76_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69922.1"
                     /translation="MKNQTKILLCFSFLSLTGCSHVVAAFIVNFQDSPATVQFWSVTT
                     PGRQCFGELQLLPLVDGTFSPDSVYSSASQIDLKTEGDHRTIALPARTALLLDRELNR
                     WDLHCVAQISKLKIITPQGTIEYTDGEVTRAFERTRAHSDSFILSIRPDARKQSFRSR
                     KDPLASHTWRIDG"
     gene            complement(1119..1370)
                     /locus_tag="PKK76_15440"
     CDS             complement(1119..1370)
                     /locus_tag="PKK76_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69923.1"
                     /translation="MKQIIFALLLPLFTVSSCGSTNAECKKAKQQALQFATAFCLAMA
                     STYTQSSYYTPGSYFASCWLEFGGLSGMGSCSTTSSSKD"
     gene            complement(1447..1821)
                     /locus_tag="PKK76_15445"
     CDS             complement(1447..1821)
                     /locus_tag="PKK76_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNL69924.1"
                     /translation="MSPKQTVEEWVKRFNAGDLNGLIDLYTEDAVNHQVVTEPLVGRD
                     SIARMFKIEFGRAKMVCIPEHIHEAGDWAILEWKDPLGLRGCGFFQIRDGRIAFQRGY
                     FDQLTFFKLQGIPVPSEYLGSE"
     gene            complement(1902..2393)
                     /locus_tag="PKK76_15450"
     CDS             complement(1902..2393)
                     /locus_tag="PKK76_15450"
                     /inference="COORDINATES: protein motif:HMM:NF022606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 domain-containing protein"
                     /protein_id="HNL69925.1"
                     /translation="MNEWSMVALRIVAIGVGATLVMDLWTIILKLFGVKSLNYAHLGR
                     WIGHIPRGKWIHESIAGSPPIKGELWIGWCAHYSIGIAFASLLVAIFGVQWTRTPSLA
                     PALLVGILTVVAPFFILQPGLGAGFASSKTPNPAFNRIKSLLTHTVFGFGLYLAGLLT
                     SSL"
     gene            complement(2390..2614)
                     /locus_tag="PKK76_15455"
     CDS             complement(2390..2614)
                     /locus_tag="PKK76_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69926.1"
                     /translation="MPAEKEDSNTRYFIDIDLLSRKIIQISYAQRQGLPQDLPLPAQR
                     VFITKGQFKKLQKKRDLVETKPARRRDKNV"
     gene            complement(2657..>3323)
                     /locus_tag="PKK76_15460"
     CDS             complement(2657..>3323)
                     /locus_tag="PKK76_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69927.1"
                     /translation="GRTGTHRSQTKWSVYRRALQCNQMIKKLTVTGVIGACVCIYECS
                     TPVVTKHVAGDSVACVDHSSLILWGTAWRSNQKEPERRRSIARQAITNFFTTNFAIAP
                     DSILEQIQGRDPVALSDTEILNFAQTSTHKWRQIVLIRVEELGPLIVIYPSPILWEGG
                     TETRMRIRVLDIAPPALKTDVSLHRRDTGAFVLRGTGSLNHDLEFTLKEVFGEKCESR
                     QIR"
BASE COUNT          769 a          817 c          871 g          866 t
ORIGIN      
        1 ggtgtcccag agcttcatgt tcgctttcta taatgaaggg ccatctgccc ctgagcaaat
       61 cgtttgctct ccagtagttc caggggaatc gattgatcct gttttatagt gtcaaacagc
      121 cgcggtcctt tgcccgcaac cacaggttga accacaaaat gatattcgtc aatgagtccg
      181 gctcgcgata gctgagatgc gatgctgaga ctgcccacac agattggctt acctggtttt
      241 tgtttgagtg cgatcacttc cccttcgagg ttctcacgga cgattcttgc atttccttct
      301 gcttgcttca gggtgcgcga aaatacaatc ttgtccagag aatcgaaaac gcgcgcgaat
      361 tcattggatg tcctggacat agactggttt ttggccacgt caggccagaa cggaaccata
      421 agctggtatg tgacgcgacc gtacaggatg gtgtccgtcg ctcgcagaag ctctgtgaag
      481 tattcgtgca actcttcgtc tgcgattccg tctgtgtggc tgcagtgtcc atctgctgtg
      541 atgttgattc caaaaatcag ctttcgcatg aatttctccg gttgcgttca accatcgatc
      601 cgccaggtgt gactggcaag aggatctttc ctgcttctaa aggattgctt acgcgcgtcc
      661 gggcgtatgg atagaatgaa tgaatcactg tgcgcccgcg ttctctcaaa cgcccgcgtt
      721 acttctccat ctgtgtactc tatggttccc tgaggtgtta tgattttgag tttggaaatt
      781 tgcgcgacgc aatggagatc ccaccggttc aattctcgat cgagaagaag ggcagtacgc
      841 gccggcaacg caatcgttct gtgatctcct tcggttttca gatcgatctg cgatgcagac
      901 gaatagacag agtccgggga aaaggttccg tccacgagcg gaaggagttg aagttcacca
      961 aagcactggc ggcccggggt ggtgacagac cagaactgga cggttgcagg cgagtcctga
     1021 aagtttacaa tgaatgctgc cactacgtgc gaacatcccg tcagggataa aaaggagaaa
     1081 cataggagga tctttgtttg attcttcaag gtccaatctc agtctttgct cgaacttgtt
     1141 gtgctgcagg accccatgcc gctgagtcct ccgaattcca gccagcaaga cgcgaaataa
     1201 gatcctggtg tgtaatagga ggactgggtg taggtggaag ccatggccag gcagaaagca
     1261 gttgcgaatt gcagtgcttg ctgtttggcc tttttacatt ctgcattcgt ggaaccgcaa
     1321 ctcgataccg taaagagagg caagagcaag gcgaaaatga tctgtttcat ggaaatcctc
     1381 ctgtccagga atggtgttct ctatgcgagc gagtcagatt gtcgcctgac ggcaaataag
     1441 cttcggctac tctgagccca ggtattcaga agggactgga attccctgca gtttgaaaaa
     1501 cgtaagttga tcgaaatagc ctctttgaaa agcaatgcga ccatctcgaa tttgaaagaa
     1561 gccgcacccg cgcagaccca gggggtcttt ccattccaga atcgcccagt caccggcctc
     1621 gtggatatgc tctggaatac agaccatttt cgcgcggccg aattcaatct tgaacatgcg
     1681 cgcaatggag tcgcggccca cgagcggttc tgtgactacc tgatgattga ccgcatcttc
     1741 tgtgtacagg tctatcaggc cgtttagatc tcccgcattg aatctcttca cccattcttc
     1801 cactgtttgt ttaggagaca tctggtttga tatttcgata ccattcgcgt cttgcaatct
     1861 gattagttgt taggcgaaag ggaatgatga gactatcgag attacagtga gctcgttaga
     1921 agtccggcca gatacagccc gaatccaaag acagtgtgcg ttagtaggct cttgattcga
     1981 ttgaaggctg gattgggggt tttggaggat gcgaagcccg ctcccagtcc cggctgcaga
     2041 atgaaaaatg gcgccacgac cgtgagaatg ccaaccagaa gggccggcgc caaactcgga
     2101 gtgcgagtcc actgtactcc gaagatggct accagaagag aagcaaacgc aattccaatg
     2161 gagtaatgag cgcaccaccc aatccagagt tctcctttga ttggtggaga accggctatg
     2221 ctctcgtgga tccactttcc gcgaggaatg tgcccaatcc atctacccag atgcgcgtaa
     2281 ttgagtgatt taactccaaa tagtttgagt atgattgtcc agaggtccat gacaagagtg
     2341 gcgcccactc cgatggccac gatgcgcaga gcgaccatgg accattcatt catacatttt
     2401 tgtcccgacg ccgggctggt tttgtttcca caaggtcccg tttcttctgc aatttcttga
     2461 actggccttt tgtgatgaac actctttgag cgggcaacgg gaggtcctgc ggcaagccct
     2521 gtctttgtgc gtaacttatc tggatgattt ttctcgacag aaggtctatg tctatgaaat
     2581 atctggtgtt actgtcttct ttttctgccg gcattttgtt atccttgaaa tcgtttatca
     2641 gatgttaggc gagtgactag cgaatctgtc gcgactcgca tttctccccg aagacttcct
     2701 tgagcgtgaa ctccaggtca tgattgagag accctgttcc acgcagaaca aaagcgcctg
     2761 tgtcccttct atgaagggac acatccgtct tgagcgctgg cggagcaatg tccaggactc
     2821 tgattcgcat tctggtttcc gttccaccct cccatagaat gggggaggga tagatgacaa
     2881 tgagcggacc cagttcctct actcggataa gaacgatttg cctccacttg tgtgtagatg
     2941 tttgcgcgaa gttcagaatc tccgtatccg acaaggcaac cggatcgcgg ccctggatct
     3001 gttccaggat cgaatccggc gcgatggcaa agttcgtggt gaagaaatta gtgatcgcct
     3061 gacgtgcgat ggaacgcctt ctttcgggct ctttctgatt ggatcgccat gctgtgcccc
     3121 agaggatcag ggaagaatgg tcgacgcaag cgacggagtc tcctgcaacg tgttttgtca
     3181 ccacaggagt ggagcattcg tatatacaga cacaagcgcc gatcacacct gtcacggtta
     3241 gtttcttgat catttgatta cactgtagcg cgcgtctata aacggaccat tttgtctggc
     3301 ttctatgagt tccagtgcgg ccg
//
LOCUS       DAORFF010000451         3322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_18526_length_3322_cov_3.229875, whole genome shotgun sequence.
ACCESSION   DAORFF010000451 DAORFF010000000
VERSION     DAORFF010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18526_length_3322_cov_3.229875"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="PKK76_15465"
     CDS             complement(<1..1017)
                     /locus_tag="PKK76_15465"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNL69928.1"
                     /translation="MQSVSCPKCSHKFELSKALESEMEAKLRESLEEEQTRWKQEEAS
                     KLEKRLRAEAKDRLSVEMQALQESVAEKEKALREARSSELEVRRKMRSLEEKSAAFEI
                     EKQNILDDERKKIALQAELAAEEKYKLKIVEMQTERTSLASRIDELQKKAEKTSQELQ
                     GTAQEIGLMDLLQKHFPGDRFERVGKGKKGGDVIHSVVVNGKVCARIVYESKRTAAWS
                     NTWIEKLRTDRNNEKADLAIIVSQALPDSIRSAGCMEGIWICDFQTAPWLSAALRNGL
                     IELARVRSAMESQSDMKDIVWRYLTGNEFKNRIERMLRTFMSMKQQIERERAQALKWL
                     SERDQ"
     gene            complement(1102..1488)
                     /locus_tag="PKK76_15470"
     CDS             complement(1102..1488)
                     /locus_tag="PKK76_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69929.1"
                     /translation="MKKLHLAVGIALLLVFVSTGAFMKWEQLHTDGLDPLYRMMHRSR
                     HIYILFAGLIHLMAYRAYDDGLSGTRSILLRISSGFLFASAVLFPIAFLVEPQLHRVA
                     APVTFFGVLLTLIGVLLPVVSGFMRK"
     gene            complement(1566..2474)
                     /locus_tag="PKK76_15475"
     CDS             complement(1566..2474)
                     /locus_tag="PKK76_15475"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNL69930.1"
                     /translation="METFLRIVLGILFLPLVSCQQTPAGMAKLPSGTYFRGLQISRKA
                     DQRPRHKVRIRSFYLDETLVTVADFRRFVERTGYQTSAERMGYAMISLEGMADWKWKQ
                     ARGASWKNPFGELRNVPVHEEDPVTAVSWEDANRYCAMVGKRLPTEAEWEYAARAGSD
                     LRFPWGDSPMRKGAFGLNFWQTEHHEHGSLEDGYLYLSPVRAYPPNDWGMYDPVGNVW
                     QLTADYYSPYAYEDAASEADQNPDRAVANPKGPDHGYERVARGGSWWCSENTCNGFGL
                     YYRGHTAPVAPFNNQGFRCAKDLPVD"
     gene            complement(2490..3095)
                     /locus_tag="PKK76_15480"
     CDS             complement(2490..3095)
                     /locus_tag="PKK76_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNL69931.1"
                     /translation="MTAPSGLYVRKEADAKSKPVALLKKGAYFHFSGVAGAPVTIDSI
                     SSRWLKYSGPAGTGYVFGGFVSREPPLLVYTREQINEIEKENRAYVESLAREELRRME
                     EAQKAGQFYQNCDACEHLKIDWRRVELRAGCDQNARERIHQLVIERIPGYDKRGINDE
                     ATRQAFLDAAEKVDNPYCEQLLMNSWQSQGPAQCNGPSFFE"
BASE COUNT          769 a          893 c          780 g          880 t
ORIGIN      
        1 ctggtcgcgt tctgaaagcc atttcaatgc ctgggcgcgc tcgcgttcaa tctgctgctt
       61 catggacatg aatgtgcgaa gcattcgctc aattctattt ttaaactcat ttcctgtgag
      121 ataccgccat acaatgtctt tcatgtcaga ttgactttcc atcgcagagc gcactcgagc
      181 aagctcaatg aggccgtttc gcagagcagc actgagccag ggagcagtct gaaaatcgca
      241 gatccagatt ccttccatgc aacccgctga tcgaatggag tcaggcagtg cctgagatac
      301 aatgatggca agatccgcct tttcgttgtt tctgtccgtg cgcagtttct caatccaggt
      361 attcgaccag gctgcagttc gtttgctttc ataaacgatt ctagcgcaga cttttccatt
      421 tacgacgaca gagtgaatga catcccctcc tttttttcct ttgcccactc gttcaaagcg
      481 gtcccccgga aaatgcttct gcaagaggtc catcagtcca atctcctgag cggtgccctg
      541 caattcctga ctggtcttct ccgctttttt ctgtagctcg tctattctag atgcaagaga
      601 agtgcgctcc gtttgcattt ccacgatctt gagtttgtat ttttcctctg ctgccagttc
      661 cgcctgcaag gcgatcttct tgcgttcgtc atcgaggata ttctgctttt caatttcaaa
      721 ggcggcggac ttttcttcga gggaacgcat ctttcgtcgc acttccagtt ctgaagatcg
      781 agcttctctc agcgcttttt ctttttctgc gacggactcc tgcaatgctt gcatttcaac
      841 gctgagccga tcctttgctt ccgcacgcag tctcttttcg agcttgctgg cttcctcctg
      901 tttccatctg gtctgctctt cctccaggct ttcgcgaagc tttgcctcca tttcactttc
      961 gagcgctttg ctcagttcga acttatggga acatttggga cagctgacag attgcatctt
     1021 cacgggggcc tccagatgac tggattgtat ccggggggtg tgacatgttt ggatacggaa
     1081 atgtagatct tacccggggc gctattttcg cataaatcct gaaacgacag ggagaagaac
     1141 accgatcaaa gtaagcaaca ctccgaagaa agtcactgga gccgcgactc ggtggagctg
     1201 gggctctact agaaacgcaa tgggaaaaag gacggcagat gcaaagagaa atcccgagga
     1261 aatccttagc aagatcgatc ttgtgccact taagccgtcg tcgtaagcac gatacgccat
     1321 caaatgaatc agccctgcaa acaggatgta aatgtgcctg cttcgatgca tcattctata
     1381 gagtggatcg agcccatcgg tatgaagctg ctcccatttc atgaacgccc ccgtggaaac
     1441 aaaaacaagt agaagtgcga tgccgacggc tagatgaagt ttcttcacaa tgagtgatcg
     1501 cttttcatga tgatgctgtc aaggggcttc tctctcaagc aaaggccgca acgtttcgag
     1561 gcagactagt cgaccggtag gtccttcgcg caacggaaac cttgattgtt gaaaggggcc
     1621 acaggggctg tgtgtccgcg gtagtacaat ccaaaaccat tgcacgtatt ttcggagcac
     1681 caccacgatc caccgcgcgc cactctctcg tatccgtgat cgggaccttt tggatttgca
     1741 accgcacgat ctggattctg atccgcctcc gatgctgcat cctcgtatgc gtaaggacta
     1801 taatagtcag ccgtcaattg ccacacgttt ccgactggat cgtacatccc ccaatcattt
     1861 ggcggatacg cgcgcactgg agaaagatac agatatccat cttctagact gccgtgttcg
     1921 tgatgctctg tctgccagaa gttcaatcca aacgctccct ttcgcatggg cgaatcgccc
     1981 cagggaaatc tgagatcgct tcctgctctt gcggcgtatt cccattccgc ttccgtcggc
     2041 aatctcttgc cgaccattgc acaatatcga tttgcatctt cccatgaaac agcggtcaca
     2101 ggatcctctt cgtgcacagg aacattgcga agctctccaa atggattttt ccagctggct
     2161 cctctggctt gcttccattt ccagtctgcc atcccttcca atgaaatcat cgcataaccc
     2221 atgcgttcgg cggacgtttg atagcctgtg cgttctacga acctgcgaaa atcagcaacg
     2281 gtaaccagag tctcatccag atagaacgaa cgaattctca ctttgtggcg cggcctctgg
     2341 tctgctttgc gcgatatctg caagcctctg aaataggttc ccgatggaag tttcgccatg
     2401 cccgcgggcg tttgctggca gcttacgaga ggaaggaata gaatcccgag gacgattcta
     2461 aggaacgtct ccattcggat actcgaccat cattcaaaga aagaagggcc attgcattgt
     2521 gcgggccctt gactttgcca ggaattcatc agcaattgct cgcaataagg gttgtccact
     2581 ttttcggcgg catccaggaa agcctgacgc gtagcctcgt cattgattcc gcgtttgtca
     2641 taaccgggaa ttcgttcgat caccaattga tggatgcgct ctcgggcatt ttgatcgcag
     2701 cctgcgcgca attctacgcg tctccaatca atcttcaaat gctcacaggc gtcgcagttc
     2761 tggtaaaatt gccctgcttt ctgcgcttct tccatcctgc gcagctcttc tcgcgcgagg
     2821 ctttctacat acgcccgatt ttctttttca atttcgttga tctgctctct ggtgtagaca
     2881 aggagcggag gctccctgga gacgaaacct ccaaatacat atccagttcc tgccggaccg
     2941 gaatacttca accagcgaga tgaaatacta tcgatggtta cgggggcacc tgcgacgcca
     3001 gaaaaatgaa aatacgctcc ctttttcaga agggccacag gtttgctctt cgcatctgct
     3061 tccttgcgca cgtacagtcc agatggcgcc gtcacgtaga gaaactgtgg aatggacaga
     3121 tcgtcaatct cgctggttgt gatggcggag tgccctgaag agcgaaaaga aatggaatca
     3181 ggaagatttt tgaacgacgg tgcattactc tgccaatgag agcaattccc cgctggaaat
     3241 gtgaactgtc aatacaagtt aaactacatt tcctgtaaac aggaggtaca ccgtaaacaa
     3301 aaagacaatc ccaaatttca tc
//
LOCUS       DAORFF010000452         3321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_18534_length_3321_cov_3.708206, whole genome shotgun sequence.
ACCESSION   DAORFF010000452 DAORFF010000000
VERSION     DAORFF010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18534_length_3321_cov_3.708206"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="PKK76_15485"
     CDS             complement(<1..1161)
                     /locus_tag="PKK76_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69932.1"
                     /translation="MDEMQFDRTRRAIGSDKLQDKDRQQLIQKFTSAGGQVMDERALR
                     REAERAEKARAKGGAAGEEDKRARNLTGPRLPSQVAREQKFAEADQIQKIRRIRAEEE
                     REASSFVSRLVVKLRCKFKNIVPFGKDRVLPEFMSRLNLDARRAVMECNILSMDLFST
                     NPEIGRQIIKDLDSKNPLYVEILDRAGKLYDRAELSDLLSAYTSDPTASAAFDGVRVP
                     AISFLKKLYYLRPYQETYLVAAQAAIELQEKLEKKPSNLYAAKKKKIRQEWRTLMDDI
                     FPLLVLLLQRMELKKVEPGTWLFEEIIQANPDERPGRRRAGEPISDLQAKQAEQKAAE
                     EKQEDPDEEKIEDAVLEDGEEKPASDSKETEIAYGMTLMSQIPIENMKRKFDV"
     gene            1192..1551
                     /locus_tag="PKK76_15490"
     CDS             1192..1551
                     /locus_tag="PKK76_15490"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNL69933.1"
                     /translation="MQSKINQDEFLKQEVLRLETAGCLIAGALIFGSQASGEARPDSD
                     VDLGIIYEGTAPEMDRTDTDVFVWSVARWDSGFPLQIEIARKGRILYDPRGIVLARLT
                     MIREQILPYWEIHLKRL"
     gene            1639..2574
                     /locus_tag="PKK76_15495"
     CDS             1639..2574
                     /locus_tag="PKK76_15495"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNL69934.1"
                     /translation="MRDDQRFIVFSDHHRGDRGYSDYFQRCEKTYNAALGHYLERGYH
                     LVLLGDVEEFWEFHPKTVVSSYPLTYQLEREFFEDHRFTRIRGNHDSLWKNPAIVEKY
                     LHPVFPGIRVLDGLRIRVAGEKPVTVFLAHGHQGTFLSDTLDWFSLFWFRTLGSRVIH
                     PFKRKYQTPAQSFALREEHETMMFECAEAMNQGPDKIILIAGHTHHPIFMSQNRVQRI
                     RETAEILRARGGKKNRKRLAKVRAQFEYIKADFGLDELMNRRSFYFNTGCCSFDDGSI
                     TGIEIAKGKIKLIKWTDESTHAERMILGSERLSAI"
     gene            complement(2578..3027)
                     /locus_tag="PKK76_15500"
     CDS             complement(2578..3027)
                     /locus_tag="PKK76_15500"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNL69935.1"
                     /translation="MAVSKIITGLFQKKILYENSPDKINFKLAHSWLKETYWCKGISL
                     EKVEKGFRESTTVVSAHSGNRMVGIARCVSDTTRFGYIADVYVEESARGKGIARAMVR
                     NLISQSSLRDVEHWYLFTKDAHGVYRDIGFRTYDKPERLMHLAATKK"
     gene            complement(3014..>3321)
                     /locus_tag="PKK76_15505"
     CDS             complement(3014..>3321)
                     /locus_tag="PKK76_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69936.1"
                     /translation="DFFIIDSTIADLAFFSSLIFIWVARSFFHNAHALRGGQKDKPPL
                     LVTFTSMLGATAGICFIMIAQELGPAKALSGTAYAMAGSFFVSIVFFQEFARSHGRV"
BASE COUNT          776 a          813 c          844 g          888 t
ORIGIN      
        1 tacatcgaac ttgcgcttca tgttttcaat tggaatctga ctcatcagag tcataccata
       61 cgcgatctca gtttctttgc tgtcagacgc tggcttttct tctccgtcct cgagtacggc
      121 gtcttcaatc ttttcttcat caggatcttc ctgtttttct tccgcagcct tctgttcggc
      181 ctgctttgcc tgaagatcag aaatgggttc tcctgcgcga cgacgaccag gccgttcgtc
      241 tggatttgcc tggatgattt cttcaaagag ccacgtgcct ggttctactt ttttcaactc
      301 cattctttga aggagaagca cgagcagtgg gaaaatatca tccatgagag tccgccattc
      361 ctggcggatc ttcttctttt ttgctgcgta gagattcgat ggcttttttt ccagtttttc
      421 ctggagttca atggccgcct gggccgcaac cagatacgtt tcctgataag ggcgcagata
      481 ataaagtttc tttaggaatg atatggcggg aacgcgaaca ccatcaaacg cagcagatgc
      541 ggtaggatcc gacgtgtagg cgctcaacag atctgaaagc tctgcccggt catacaattt
      601 gcccgctcga tccaggattt caacgtagag aggattcttt gagtccaggt ctttgatgat
      661 ttgtctgcca atctccggat tcgtgctgaa caagtccatg ctcaggatat tgcactccat
      721 cactgcacgg cgcgcgtcca ggttgaggcg tgacatgaac tccggcagca cccggtcttt
      781 tccaaatggg acaatgttct tgaatttgca gcgcagctta actacgaggc gggaaacaaa
      841 tgaggaggct tcccgctctt cttccgcgcg aatcctgcga atcttctgga tttgatctgc
      901 ttctgcgaac ttctgttcgc gagccacctg cgaagggagc cttgggcctg tgagattccg
      961 cgcgcgcttg tcctcttctc ctgcagctcc gccctttgcg cgtgcctttt ctgctctttc
     1021 tgcttcgcga cgaagggcac gctcgtccat aacctgcccg ccggcagacg taaatttctg
     1081 gatgagttgt tgacggtcct tgtcctggag cttatctgag ccgattgccc gacgagttcg
     1141 gtcaaactgc atttcgtcca tattgattac tgcatgaaaa aaagagaacc agtgcaatca
     1201 aaaattaacc aggacgagtt cttgaaacaa gaagtgctgc gcctggagac ggctggttgt
     1261 ctcattgccg gcgccctgat tttcggttct caggcaagtg gagaggccag acccgattca
     1321 gatgtcgatc tgggcatcat ttacgaaggg acagctcctg aaatggatcg aacggacaca
     1381 gacgtgttcg tatggagcgt ggcccggtgg gacagtggat ttcctcttca gattgagatt
     1441 gcgcgaaaag ggcgcatttt gtatgatccc cgcggcatcg tgctggccag actcactatg
     1501 atccgggagc aaattcttcc ctattgggaa atccatttga aacgactttg acatcttatg
     1561 gttgagttct taaacagccg tgacgcaatt ctccggcaat ttctatccac atacgcttca
     1621 tcgccggaga ttcaggccgt gcgcgatgat caacgcttca ttgtattttc agatcatcac
     1681 cgcggggatc gcggctattc ggattatttt caacgctgcg agaagacata caatgcggcc
     1741 ctggggcatt atctggagcg tggctaccac ctggtgctgc tgggagatgt agaagagttc
     1801 tgggagtttc atccgaagac ggtcgtttca agctatcctc tcacctatca actcgagcgc
     1861 gaattctttg aggatcatcg ttttacaaga attcgcggaa atcacgacag cctgtggaag
     1921 aatcccgcca tcgtagaaaa atatttgcat ccagtctttc cgggcattcg cgttctcgat
     1981 ggtcttcgta ttcgagtggc gggtgaaaag ccagtcaccg tctttctggc tcacggacat
     2041 cagggaacct ttctcagtga cacgctcgac tggtttagtc tgttctggtt tcgcactctg
     2101 ggctctcgcg tcattcatcc gtttaagaga aaataccaga cgccagcgca gagcttcgcg
     2161 ctccgcgagg aacacgaaac gatgatgttt gaatgcgcgg aagcaatgaa tcaggggccg
     2221 gacaagatca tcttgattgc aggtcatacg caccatccaa ttttcatgtc gcaaaatcga
     2281 gtgcagagaa ttcgggagac tgcagaaata cttcgcgcgc gcggtggaaa aaagaacagg
     2341 aaacgcctgg caaaggttcg cgcgcaattc gagtacatca aagcggattt tggtctcgat
     2401 gaattgatga atcgtagatc tttctatttc aatacagggt gttgttcctt tgacgacggt
     2461 agcattaccg gcattgaaat tgcgaaggga aaaattaagc ttatcaaatg gacggacgaa
     2521 tccactcacg cggaaagaat gattctggga tctgagaggc tatctgcgat ttaagtttta
     2581 tttcttcgtc gcggcaaggt gcatcagtct ttctggtttg tcataagtgc gaaagccaat
     2641 gtcgcgatac actccatgcg catctttcgt aaatagatac cagtgttcca catctcgaag
     2701 ggatgattgt gaaatcagat ttctgaccat ggcgcgggca attcccttcc ctctcgcgga
     2761 ttcttcaacg taaacgtcag caatgtagcc aaagcgggtt gtatctgaaa cgcatctggc
     2821 gatcccaacc atcctgtttc cgctgtgtgc gctcaccact gttgtggatt cgcgaaatcc
     2881 cttctccact ttctcgaggg aaataccttt gcaccagtag gtttctttga gccatgagtg
     2941 cgcgagtttg aagttgattt tgtccggtga attttcgtac aaaatcttct tctgaaagag
     3001 gccagtgatg attttagaca cggccatggc ttcgtgcaaa ctcctggaaa aagacaatag
     3061 atacgaagaa gctgccggcc atagcgtacg cggttccgct gagtgctttg gccgggccca
     3121 gttcctgagc gatcatgata aaacaaatgc cggccgtggc acccagcatc gatgtgaatg
     3181 taacgagtag cgggggttta tctttttgtc cgccacgcaa agcgtgtgcg ttgtgaaaga
     3241 aggagcgtgc tacccagatg aagatgaggc tggagaagaa ggcaagatcc gcaatcgtag
     3301 aatcgataat aaagaaatca t
//
LOCUS       DAORFF010000453         3313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_18600_length_3313_cov_3.455187, whole genome shotgun sequence.
ACCESSION   DAORFF010000453 DAORFF010000000
VERSION     DAORFF010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18600_length_3313_cov_3.455187"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PKK76_15510"
     CDS             complement(<1..700)
                     /locus_tag="PKK76_15510"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNL69937.1"
                     /translation="MPALVTGASGHIGSNLVKQLLDQGEKVRALIRKSSNTACLPDHK
                     NLEIVYGDVYDRPSLVAAVTGMPFVYHTAAVYSLSRKNPGQILETAMQGTRNILEAVK
                     VAGGVKRVVYTSSVAAVGMTDDPTRPLDESNWTADSDPDEYTRAKLESEKLAHSLARE
                     FGIDLVAVNPALVLGALDLKPTPSNKVVLDFLQGKVPAYLETGFSAVSVEDVASGHIL
                     AMQKGRSGERYILSG"
     gene            complement(748..1704)
                     /locus_tag="PKK76_15515"
     CDS             complement(748..1704)
                     /locus_tag="PKK76_15515"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNL69938.1"
                     /translation="MKVFVTGGSGFVGERLIRRLKADKHEVVALARGSSQAEKIKAMG
                     AQPVHGTFSNISEWETALDGIDAVVHCAAPVEFWGKWEDFYRDTVQATIDLYQAASRR
                     KVGRFIQISSESVIQDKLPLLDIDETAPYPSVPSSVYGRAKKETEIALCAQPTGPVRI
                     ILRPTFIWGKGVKALDTMAQKVTSGEFMWIDHGQTIIEMVHVDNVVQAIVLSLGAGSD
                     KSIYYVTDDRPMKVFDFFSALFAAAAIPIPKKNMNGKIAALAAGAIEGVWKLFGIQKS
                     PPLSRFELAFVAQPRRYNIAKIKRELGYSPVKTLEAGLAEMR"
     gene            1782..2486
                     /locus_tag="PKK76_15520"
     CDS             1782..2486
                     /locus_tag="PKK76_15520"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNL69939.1"
                     /translation="MATKKATKKRMPAAARRQLILESAMQLFAKKGFHGTTTADLARV
                     AGVSEALIFRFFPDKESLYREVQFLCVEARGAADSMLDQARPGTENLVRAVLILIYEV
                     FSGFGGRTKNETLRRLQTHSMLEDGKFARAFFKSHFKVYFPFIDRCLKSAHRSGDLEP
                     GGMPLECVIWFTHHLPMMVRISILSHRADYFASSDAELLESMALFCLRGIGLKSSAIK
                     KNLPRNWTEQFNLLKG"
     gene            2512..>3313
                     /locus_tag="PKK76_15525"
     CDS             2512..>3313
                     /locus_tag="PKK76_15525"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNL69940.1"
                     /translation="MRATVLGSGSFGTALASVLADKNAEVWIWSRSPDMARAIHEQHE
                     NTKYLPGFRLADNIKSSPDMAECLKDTDLIVFGIPSQSLTQVLKENTHILPEGVPIVS
                     AAKGVDRATLRLVSEIFEEELPGKFHKYLSYLSGPSFAKEILKQMPTVVSIASRNEEI
                     AKQVQQMFAHTWFRTYWTPDVTGVEVGGAIKNVIAIAAGVSDGLGLGHNTRAALITRG
                     LAEITKLGVRKGADPLTFLGLAGLGDLVLTCTGDLSRNRTVGFKLGQGM"
BASE COUNT          764 a          841 c          856 g          852 t
ORIGIN      
        1 cacccgagag gatgtatctt tctccggagc gccctttttg catggcaaga atgtgtccgg
       61 aagcaacatc ttccacgctc actgcactga atccagtttc cagataggcc ggcacttttc
      121 cctgtaagaa atccaggact actttattgg atggcgttgg tttgagatcc agcgcaccga
      181 gcaccagcgc gggattgact gcgaccagat caattccaaa ttcgcgcgcc agagagtgtg
      241 caagcttctc cgattccaac ttcgcgcgcg tgtattcatc cggatccgaa tctgcagtcc
      301 agttcgattc atccagaggg cgagttgggt cgtctgtcat tccaactgca gccacagaac
      361 tggtatagac aactcgcttc acaccgccgg ccactttgac tgcttctaga atgttgcgcg
      421 tgccctgcat ggctgtttcg agaatctgtc caggattctt tctggagaga gaatacacag
      481 ccgcagtgtg ataaacgaaa ggcattccag tgactgcagc aacgagtgag gggcgatcgt
      541 atacgtctcc gtagacgatt tccagattct tatgatccgg aaggcaggcg gtgttgctgg
      601 atttgcgaat gagagcccgc actttttctc cctgatccag gagttgtttc actagattgc
      661 ttccaatatg gcccgatgcg ccggtgacga gtgctggcat gtgtcagtta gacatgtgcg
      721 gatcaccgac gcaaggagga aatgcaatca acgcatttcc gcaagacctg cttccagagt
      781 cttaactgga gagtatccta gttctcgttt gattttcgca atgttgtacc ttctgggttg
      841 cgccacgaat gcgagttcga atcgggagag tggcggagat ttttgaatcc cgaataattt
      901 ccatactccc tcaatggcgc ctgcggcgag ggctgcgatc ttcccgttca tattcttttt
      961 gggaattgga attgccgccg ccgcaaaaag agcagagaag aaatcaaaaa ctttcattgg
     1021 tctgtcatcc gtgacgtagt agatagactt gtcgctgcct gccccgagcg agagcacaat
     1081 ggcctgcact acattgtcta cgtgtaccat ttcaatgatg gtttgcccat ggtcgatcca
     1141 catgaattcg cctgacgtta ctttctgagc catggtatcc agcgctttga ctcccttgcc
     1201 ccagatgaaa gtagggcgaa ggatgattct tacgggcccg gtcggctgag cgcagagtgc
     1261 gatttctgtt tctttcttgg ctctaccgta aacagaactc ggaacagacg ggtacggtgc
     1321 tgtttcatca atatcaagaa gcggcaactt gtcctggatc actgactcag agctgatctg
     1381 aatgaagcgg cccacttttc tgcgcgaggc agcctggtac aggtcgatgg tcgcctgcac
     1441 tgtatcgcga taaaagtctt cccattttcc ccagaactca acgggagccg cgcaatggac
     1501 caccgcatca attccgtcaa gagccgtttc ccattcggaa atatttgaaa acgttccgtg
     1561 aaccggctgt gctcccattg ccttaatttt ttctgcctgg gagcttcctc gggccagtgc
     1621 caccacttca tgtttgtctg ctttgagtct gcgaatgagc ctttctccaa cgaagccgct
     1681 tccgcctgtt acgaatactt tcatagtccc tccatgtctt gcttgactta ctttccctgt
     1741 tccataagta agtaaatact cacttacatc aaggaaaagt cgtggcaact aaaaaagcaa
     1801 ctaaaaaacg tatgcctgcg gcagcgcgtc gtcagctcat tcttgaatcc gccatgcaat
     1861 tgttcgcgaa aaagggcttc cacggcacaa ccacagcgga tcttgcacgc gttgcgggcg
     1921 tatctgaggc gcttatcttt cgcttctttc cggacaagga gtcgctctat cgagaagtgc
     1981 agtttttatg cgtggaggcc cgtggtgccg ccgattccat gctggaccag gccaggcccg
     2041 gtacagaaaa cctggtgcgc gccgtgctga ttctgattta cgaagtcttc tctggttttg
     2101 gaggtcgcac gaagaacgaa acgcttcgca ggctgcagac tcatagcatg ctggaggacg
     2161 gaaagtttgc gcgcgccttc ttcaaaagcc atttcaaggt ctattttcct tttatagacc
     2221 gatgtctgaa atcagcgcat aggtctggcg accttgagcc gggcggtatg ccactggagt
     2281 gcgtcatctg gtttactcat catttgccca tgatggttcg catcagcata ttgtcgcatc
     2341 gtgcggacta ttttgcgtca agcgacgcgg agctcctcga gagcatggct ttgttttgcc
     2401 ttcgcgggat aggcctcaag agctctgcga tcaaaaagaa tcttccgcgc aattggacgg
     2461 agcaatttaa tttattgaaa ggataaggtg ccccttgcat tttcgacgcc tatgagagca
     2521 acggtacttg gatcgggcag ttttggaacg gcgcttgcga gcgtgctggc agacaagaac
     2581 gcagaggtat ggatctggag tcggtctcca gatatggcgc gcgccattca tgagcagcac
     2641 gaaaacacta agtatttacc aggttttcga ctggcagaca atatcaagtc ttcgccggac
     2701 atggcggagt gtctcaagga tacagatctc attgtatttg gaatcccctc tcagtctctt
     2761 acgcaggttc tcaaagaaaa cactcatatc ttgccggaag gagttcccat tgtatcggca
     2821 gccaaaggcg tcgaccgtgc tacgcttcgc ctggtgagtg agattttcga agaagaactt
     2881 ccggggaagt ttcacaaata tcttagctat ctttccggtc ccagttttgc aaaagagatt
     2941 ctcaaacaga tgccaacggt tgtgagcatt gcctcgcgca atgaagaaat cgcaaaacag
     3001 gtccagcaga tgtttgctca cacatggttt cgcacttact ggacacccga tgttaccgga
     3061 gtggaagtgg gtggtgcaat caaaaacgta atcgcaattg ctgcaggtgt ttcagacggt
     3121 ctaggtctcg ggcacaatac tcgcgcggca ctcatcacaa gaggtcttgc tgaaatcaca
     3181 aagcttggtg tgcgaaaggg ggcggacccg ttgacctttc ttggtctggc tggactcggg
     3241 gatctagtgt tgacttgcac tggagacctt tcgcgcaatc gcacagttgg cttcaagctt
     3301 ggacaaggaa tga
//
LOCUS       DAORFF010000454         3312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_18616_length_3312_cov_3.351243, whole genome shotgun sequence.
ACCESSION   DAORFF010000454 DAORFF010000000
VERSION     DAORFF010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18616_length_3312_cov_3.351243"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PKK76_15530"
     CDS             complement(<1..450)
                     /locus_tag="PKK76_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNL69941.1"
                     /translation="MAESRKVYLETYGCQMNEYDSGLVRKILEGSYSAVGEPEQADLI
                     LLNTCAIREKAHEKVYSRLESFHILKRKRPDLVIGVLGCMAQNLGEDLFSQGLPLDFI
                     AGPDNYRNLPELIESSQSGSSERMHTRLSQDETYGDIQPTVTNGVLAS"
     gene            530..1183
                     /locus_tag="PKK76_15535"
     CDS             530..1183
                     /locus_tag="PKK76_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69942.1"
                     /translation="MRAADKDLTTEEIRYYHSLLQEELTDFDCGTLCAPDNNGTPYCC
                     KSEHAVPLLYKAEFELLTSMGDLWHVWKPVTSEDREIASTASRDQIFCECKGIEHCIR
                     DQRSISCRTFPLEPYLDRRGVFVGLTFVRDFTEKDPDTGKVKCPMTRRKKEIRQAFVD
                     MHFMFWEKILLRRLDEYETYIDTSKRLRRERNRTGRQFHVLFPSHFLDSKTVKQFLY"
     assembly_gap    1333..1432
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1433..1818
                     /locus_tag="PKK76_15540"
     CDS             <1433..1818
                     /locus_tag="PKK76_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69943.1"
                     /translation="SGIGIKETLELAKELPRDVLEDWIKKRGISDALVLVPGLGPANA
                     AALLKTLGTAKASAVLRTLSAEEVVLLMRDVKKMKLPRLNKKQLAKLEKPAAAKAKIS
                     AKHSKHPKPTAAKKGRVKSAHKSGS"
     gene            complement(1845..2813)
                     /locus_tag="PKK76_15545"
     CDS             complement(1845..2813)
                     /locus_tag="PKK76_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNL69944.1"
                     /translation="MNGKIKLGLIGTGHMGQYHVNVALGLPTDYEVAGIFDASPTRRT
                     EISERFGVPAFEDVGALMDAADAVVVAVPTALHYEVARQALEKGKHVLVEKPMTTDVE
                     HARELVELAESKGLILQVGHVERFNGAVLELRRVVDKPVLIESRRLAPNSGRIQDVGV
                     VMDLLIHDLDIIMNLVGEEVTSVEARGVARTGKHEDVATAVLQFEGGCVATITASRLT
                     QSKIRTLTITQESAYILLDFSTQDIDIHRQATSAYLMTREELKYRQESFVEKIFVHKD
                     NPLRQEHMHFVKCVRGQETPIVSGASDIRTIEIASRILTQIHGHHP"
     gene            complement(2824..>3312)
                     /locus_tag="PKK76_15550"
     CDS             complement(2824..>3312)
                     /locus_tag="PKK76_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNL69945.1"
                     /translation="VEKKRTLNIKIPSGVESGSRLKISGEGEAGPNGAARGDLYVVTH
                     IKRHPVFEREGNDIILEAPVPVTLAMTGGEIDVPTIDGKTVKMKIPAGTESGQIFRLK
                     ERGMPYMGGYGKGDQHVVIQVKIPKNMSRKARELVQELSKELTEASYGESVFSRIKAG
                     FR"
BASE COUNT          764 a          850 c          779 g          819 t
ORIGIN      
        1 ggatgccaga actccatttg taacggttgg ttggatgtcg ccataggttt catcctgaga
       61 tagcctcgtg tgcattcgct cgctgcttcc gctctggcta gactcaatca attcagggag
      121 attgcggtaa ttgtccgggc cggcgatgaa atccagagga agaccctgcg aaaacaaatc
      181 ctctcctaga ttctgtgcca tgcatcccag cactccgatg accagatccg ggcgtttgcg
      241 tttgagtatg tgaaaggatt cgaggcggct atagaccttc tcgtgcgcct tttcgcgaat
      301 ggcgcaggtg ttgagtaaga tgagatctgc ctgctctggt tcacccacgg cagaatagct
      361 tccttccagg attttacgca ccagacccga atcatattca ttcatctggc agccatacgt
      421 ctccagatac actttgcgcg attccgccat gttccgtcca aagaacaaaa agctcgcacc
      481 cgttgctatc ttaatttaca gggaaccgtt cgagagagat agagacataa tgcgggcagc
      541 ggataaagac cttaccacgg aagagattcg ctactaccat tctctgcttc aggaagagct
      601 tactgacttt gactgcggca cactgtgcgc tccggacaac aatggaactc cctactgctg
      661 caaatcggag catgcagtgc cccttctata caaagcagaa tttgaactgc tcacttctat
      721 gggagatctc tggcatgttt ggaaaccagt gacctcggaa gaccgtgaaa tcgcaagcac
      781 tgcttctaga gaccagatct tctgtgaatg caagggaatc gagcactgca ttcgcgacca
      841 gagatccatt tcatgcagga cttttccact cgaaccgtac ctggaccggc gcggggtttt
      901 cgtgggtctc actttcgtgc gcgatttcac tgaaaaagat ccggacacag gcaaggtaaa
      961 atgccccatg acgcggagaa aaaaggaaat ccgtcaggcg ttcgtggata tgcacttcat
     1021 gttctgggaa aaaatccttc ttcgccgcct ggacgagtac gaaacctata ttgatacttc
     1081 caagagactc cgccgcgagc gcaatcgcac tggcagacaa tttcacgtac tgtttccaag
     1141 ccacttcctg gattcaaaaa cagtgaagca attcctctac tgatccatgg gcattcaaat
     1201 actcagcgaa agcctgggct atctgacgga actgggccct gcgcgcgtga tgaaactggt
     1261 caccggtctt tccatggacc ggttgatttt tatttcacag aaagtagacc gtaagtccgt
     1321 actcggtgta ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttctgga
     1441 atcggaatca aggaaactct ggagcttgca aaagaactgc cgcgcgatgt gctagaggat
     1501 tggatcaaga aaagaggcat ctcagatgca ctcgttcttg ttccgggact gggacctgcg
     1561 aatgcggctg ctcttctaaa gactctgggt acagcaaaag cctctgcggt acttcgcact
     1621 ctttccgcag aggaagtagt actcctcatg cgcgatgtaa agaaaatgaa gcttccacgt
     1681 ctgaacaaga agcagcttgc gaaactggaa aaaccggcag ccgcaaaagc aaaaatcagc
     1741 gccaaacact caaaacatcc aaaacccaca gccgcgaaga aagggcgggt aaaaagcgct
     1801 cacaaatcgg gctcataagg aatgatcccg atggtcaagg atgatcaagg atgatggccg
     1861 tgaatctggg tcaaaatccg acttgcgatt tcaattgtgc gaatgtctga agcgccgctt
     1921 acaatcggag tttcctgacc gcgaacgcat ttcacaaaat gcatgtgttc ctgtctgagc
     1981 gggttgtcct tgtgaacaaa gatcttctct acgaatgatt cctggcggta tttcaattct
     2041 tcgcgcgtca tcaaatacgc actggttgcc tgacgatgaa tgtcaatgtc ctgagtagag
     2101 aaatccagaa ggatataagc gctttcttga gtgatggtaa gagtgcgaat cttgctctgc
     2161 gtgaggcggc ttgctgtgat cgttgccacg catcctcctt cgaactggag cacggctgtg
     2221 gccacatctt cgtgcttgcc tgtgcgcgca acgccgcgcg cttccacaga agtcacttct
     2281 tctcccacaa gattcatgat gatgtccagg tcgtggatga gcaagtccat cacaacgcct
     2341 acgtcctgga ttctgccgct gttaggtgca aggcgacgcg attcaatcag tactggtttg
     2401 tctaccacac gcctcagttc tagaactgcg ccgttgaatc tttccacatg acccacttgc
     2461 agtataaggc ctttgctttc cgcaagctcc acaagttcgc gggcgtgctc cacgtctgtg
     2521 gtcattggtt tttcaacgag tacgtgtttg cccttctcca gagcctgccg cgccacttcg
     2581 tagtggagtg ctgtgggaac agcaactaca actgcgtctg ctgcatccat gagcgcgccc
     2641 acgtcttcaa atgcaggtac gccaaatcgt tctgaaatct cagtgcgacg cgtggggctt
     2701 gcatcaaaaa tcccggccac ttcatagtct gttggaagac cgagagccac atttacgtgg
     2761 tattgtccca tgtggcctgt gccaatgaga cctagcttga tttttccgtt cattctctgc
     2821 ctgttaccgg aaaccggcct tgattcttga gaagactgat tctccgtagc ttgcctccgt
     2881 caactccttg ctcagttcct gaaccagttc gcgcgctttg cggctcatgt tctttggaat
     2941 cttcacttga atgacaacat gctggtcccc tttgccgtaa ccgcccatgt atggcattcc
     3001 cctttctttg aggcgaaaga tctgcccgct ttctgttccc gcaggaattt tcatcttcac
     3061 agtttttccg tcaatggttg gtacatcaat ttcgccgcca gtcatcgcga gagtcacagg
     3121 cacgggtgct tctagaatga tatcgtttcc ttcgcgctca aacactggat ggcgtttgat
     3181 gtgagtgacc acatacagat ctccccgcgc agctccgttt ggtccagctt cgccttcgcc
     3241 tgaaatcttc agtctgcttc cggattctac tccggatgga attttgatat taagagtgcg
     3301 tttcttttct ac
//
LOCUS       DAORFF010000455         3310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_18631_length_3310_cov_3.619969, whole genome shotgun sequence.
ACCESSION   DAORFF010000455 DAORFF010000000
VERSION     DAORFF010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18631_length_3310_cov_3.619969"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PKK76_15555"
     CDS             complement(<1..480)
                     /locus_tag="PKK76_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69946.1"
                     /translation="MSKKYTLGLDRPAPGILERLGAKAFRSFASRRMLHRPTPSDDVL
                     VKELRVITWIGAFLAFAAGSLTTVPAVYLQVIHDGDPFAERMFWMFVPTIVMTGVEFA
                     LLFYTTVWCVVRVAEETGHGPDELDRPLFLGSDSLPNLLSRAALEIPDPVYQFLGIDP
                     "
     gene            complement(477..938)
                     /locus_tag="PKK76_15560"
     CDS             complement(477..938)
                     /locus_tag="PKK76_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNL69947.1"
                     /translation="MQYWLMKSEPDVFSIDHLAKLPGKTGSWEGVRNYQARNHLQAMK
                     KGDLALFYHSSADIIGVAGIVTIAGEAEPDTFALDKKSIYHDPKATREKNPWVFVRVK
                     YQKKFKEVVTLATLRQTAGLEDMLVLKKGMRLSIQPVTEKEFKIIEKMGSK"
     gene            991..1746
                     /locus_tag="PKK76_15565"
     CDS             991..1746
                     /locus_tag="PKK76_15565"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69948.1"
                     /translation="MFYKASDLLPFIVQLAFGFALFFAEKTGKFQLSYSKFRKGGNMN
                     PRLAMFLIYFPAPIIYTVASMSGGLKNSPYHDALFYCFLFHFGKRCLEILFVHKYSRP
                     MGFGTVLLIGSLYGSIGWTAGTLHSLVSKEVGESSGVIPFLVIGGIIFAIGQAINLYH
                     HVLLAALRKEDSTAYIVPEGGLFSKVVCPHYLGEIMAWFGYAAMSGFLPMLGIAFIMS
                     LYLTGRSLRTVSWYKENVSGFPANRRALIPGIL"
     gene            1799..2020
                     /locus_tag="PKK76_15570"
     CDS             1799..2020
                     /locus_tag="PKK76_15570"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNL69949.1"
                     /translation="MKFLGNKVRELREAREWTQIEMARRLWETGKFKGKTRQRVHQIE
                     SGEHASARTISALCEVFEVQPETFFDNEF"
     gene            2312..2941
                     /locus_tag="PKK76_15575"
     CDS             2312..2941
                     /locus_tag="PKK76_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69950.1"
                     /translation="MIDFNLGLCRIGPMAAKKSGKKSGKKAGKKAGKKAGKKKKRGRT
                     GPGSPLSVETEGGYSGVEDTVPTFSSTVSSEHTAGGEGKSSSPLLLVGLGGVVAVVLI
                     FFLFRGKNHDQSPAPSSQSAEISAPASVGTETTREYTTKQGQTLTSIAESELKDKTRA
                     KEILELNIQNIAKLKAMPENKGLEATNLKNLGVDKPFGANARKLLLPAK"
     gene            complement(3010..>3310)
                     /locus_tag="PKK76_15580"
     CDS             complement(3010..>3310)
                     /locus_tag="PKK76_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNL69951.1"
                     /translation="EVAGGVDIFPEFRTAPMAKDRITTAKEVLARNPDVIIGSWCGKP
                     MERTWIDQHLGELACVRAGNVHEIDSSIILQPGPALFLEGVDALARAIFNERLHQ"
BASE COUNT          852 a          828 c          764 g          866 t
ORIGIN      
        1 cggatcaatt ccaagaaact gatacactgg atccggaatt tcaagagccg cgcgggaaag
       61 tagattcggc aatgaatcac ttcccagaaa cagcggacga tcgagttcgt ccggtccatg
      121 gcccgtttct tccgcaactc gcaccacgca ccagacagtt gtatagaaca aaagagcaaa
      181 ctccacccct gtcatgacga tcgtcggaac aaacatccag aacattcttt ctgcgaacgg
      241 atcgccatca tgaatcacct gaagatagac ggccgggact gtggtaaggg agccggcagc
      301 gaacgcaagg aaggcgccga tccatgtgat caccctcagt tccttgacca gaacatcatc
      361 agaaggtgta ggacgatgga gcatgcgcct cgatgcaaac gatcgaaacg cttttgcccc
      421 gaggcgttcc aggatcccgg gggccggtcg atccagcccg agtgtatact tcttactcac
      481 ttgctaccca tcttttctat gattttgaat tccttttcag tcactggctg gatggagaga
      541 cgcattcctt tctttagaac gagcatatcc tcaaggcccg ctgtttgcct gagagttgcc
      601 agagtcacta cttccttgaa cttcttctga tactttacgc gaacaaatac ccatggattc
      661 ttttcccggg ttgcttttgg atcatggtag atcgatttct tgtccagagc gaatgtatca
      721 ggctccgctt ctcctgcgat cgtcacaatc ccggccacgc ctatgatgtc cgcactggaa
      781 tggtagaaga gtgcaagatc gcccttcttc attgcctgca gatgattgcg cgcctggtaa
      841 ttcctgaccc cttcccagct tccagtcttg cctggtagtt tcgcaaggtg atcgatggaa
      901 aagacgtccg gttcggattt cataagccag tattgcatga tccactccaa aatctattga
      961 cccttccgtt cacgagggcc cgctggaatc gtgttttata aggcatcgga tctactacca
     1021 tttatcgttc agctcgcatt cggttttgcg ctattttttg cggaaaaaac gggcaaattc
     1081 cagctatctt acagcaagtt tcgcaagggc ggaaacatga atcccaggct ggccatgttc
     1141 ctgatctact ttccagcccc tatcatttac accgtggcaa gtatgtccgg cggactgaag
     1201 aactcgccct atcatgatgc tttgttctat tgttttctat ttcattttgg caagagatgc
     1261 cttgagattc tgttcgttca caagtattcg cgacccatgg gatttggaac tgtattgctg
     1321 attggttcat tgtatggatc gatcggatgg actgcgggga ctctgcattc tctggtctcc
     1381 aaagaagtag gagaatccag cggagtgatt ccttttctag tgatcggtgg aatcatcttt
     1441 gctattggcc aggcaatcaa tctgtatcat catgttctac tcgcggccct tcgcaaagaa
     1501 gacagtacag catacattgt accagagggt ggattattct caaaagtagt ctgtccgcac
     1561 tatctgggtg agatcatggc atggtttggt tacgcagcca tgtctggctt ccttcccatg
     1621 cttggaattg cattcatcat gtctctgtat cttacgggca gatcacttcg aaccgtaagc
     1681 tggtataaag agaacgtatc cggattcccg gctaacaggc gcgcattgat accaggcatc
     1741 ctttaatcat tcgtgcaaaa cgtttgacat gccatttttg cagtcttatg actgtgaaat
     1801 gaagttcctg ggcaacaaag tccgcgaatt gcgtgaagcc agagagtgga cacagataga
     1861 aatggcccgt cgactatggg agacgggcaa attcaaaggc aaaacgagac agagagttca
     1921 tcaaattgaa tccggagagc atgcatctgc gcgcacaatc agtgctctat gtgaagtttt
     1981 tgaagttcaa ccagaaactt tcttcgataa tgagttctga tttttgattt tgcggatgtg
     2041 attgcatcat ttgatgctcg ctacgcgcac ttttcattcc aaaattcgtt tcagcatcac
     2101 ttcaacatca ttcatttact gaaaatcgca acgtgcatgc ctcgaaaatg cacattgaat
     2161 catcaaaaat catttttcat gcgatgataa tcgcattcga acgcgcaatg cgcacttgat
     2221 gaatgaaatt tcgcgatgaa atgcgtgcga agatgattca aaacacaaat tttgaaattt
     2281 ttttcacttt agaacccaat agtataaaaa aatgattgat ttcaatttag gattatgtcg
     2341 tattgggccc atggcagcta aaaaatctgg taagaaatcc ggcaaaaaag ccggtaaaaa
     2401 agcaggcaag aaagccggca aaaagaaaaa gcgaggccga acaggccccg gctcgcccct
     2461 ttcggttgaa accgaagggg ggtactctgg cgtagaagat acggtcccca ccttttcatc
     2521 tactgtcagt tctgaacaca cagcgggggg agaagggaag tcttcttccc cccttcttct
     2581 cgtgggcctc ggcggcgttg tcgccgtggt tctcatcttc ttcctcttca gaggaaagaa
     2641 tcatgatcaa tctcctgctc catcgtctca gagtgctgaa atcagcgcgc ctgcttctgt
     2701 aggaacagag actacacgag aatacacgac taaacaaggt caaacactga cctcaatagc
     2761 cgaatccgag ctcaaagata agacgcgtgc caaggaaatt cttgaattga acattcagaa
     2821 tattgcgaag ctcaaagcga tgcctgaaaa caagggtctc gaagcgacaa atctgaaaaa
     2881 tctcggagtg gataagccgt ttggggcaaa cgctcgcaaa cttcttcttc ccgcaaaata
     2941 atccgtaggt tcagccagat tcggtctgaa tctacggctt ttttttaccc atccctgacg
     3001 cgcttcgttc tactggtgta atctctcgtt gaagatcgcg cgtgcgagtg catccactcc
     3061 ttctaaaaac agagccggac ccggctgcag tatgatcgat gaatcaattt catggacgtt
     3121 gcccgctcga acacaggcaa gttctcccag gtgctggtcg atccaggttc tttccattgg
     3181 ctttccacac cacgaaccaa tgatcacgtc cggatttcgg gccaggacct ccttcgcggt
     3241 cgtaatgcga tcttttgcca tgggtgccgt gcgaaattcc ggaaaaatgt ccacgccgcc
     3301 cgccacctcc
//
LOCUS       DAORFF010000456         3307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_18654_length_3307_cov_3.167589, whole genome shotgun sequence.
ACCESSION   DAORFF010000456 DAORFF010000000
VERSION     DAORFF010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18654_length_3307_cov_3.167589"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="PKK76_15585"
     CDS             <1..1327
                     /locus_tag="PKK76_15585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HNL69952.1"
                     /translation="DGTIQVAGSTPFEMAKAAYSELARTTPVSPDDSLALFTGLCPFL
                     DKGIADDLAKVHTQFYAHYSYCENSPYGFLPDLVSSEFLAEAEPGATDLRDFVFKNIE
                     KYDAEILYRRPDVRQFRLDFSCGSPRSLDLSRRLRAIDPDVTYERIEKVIQDHPEIIR
                     SFPSYFEIDLTSLSKVHPILYPEISGTDSLPLALAIKLNAEIAAHGWKKDATVSLAGR
                     GEPMLHPDFLEILKLFLANESVSTVFVETYGHSIDEKQITLWNSLPGAEKLSVILRLS
                     TLKKDRYRFLYGEDLFEPAMAAVVHLEKAANRKFAAYVEMLKIKEVEDEITPFFDRFE
                     NSTVRVILNKYNRYIDLLPERRVTDLTPLDRSFCWHIARDFYLKPDGTVPLCKQDIVG
                     RAATHDFAKKTILEILSATARAHSHSVRGEHDSVGMPCLNCDEWYTFNG"
     gene            1324..2052
                     /locus_tag="PKK76_15590"
     CDS             1324..2052
                     /locus_tag="PKK76_15590"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69953.1"
                     /translation="MKCAVLIQARSASVRLPGKIYEPVPESGSPSILDHVVRRMQLAN
                     VGPVVVLIPEDDTLLADYLSVRRIEFLRGPHEDVRARYRHAARAMNLDLVVRATADNP
                     ALDPVCAARSVQEIQSRNLDLFSFSAMPLGTGVEVFTAAALLSDSLEGPEYSEHVSLH
                     IKHAPDRFRVEHSPWREGLPPELPRLTVDTREDLEVVRSVFRKLGVDFTLDQLMELFA
                     SNPELFAGNRHVVQKTFLPPGASS"
     gene            2049..2810
                     /locus_tag="PKK76_15595"
     CDS             2049..2810
                     /locus_tag="PKK76_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69954.1"
                     /translation="MILILAGAGARFGTGHVERMRILKGLLEARGHSVSLRLTDEPEA
                     ERGRHGDSLIVFDARDSACDVGERVIAVDNRSSIRAGNTSIVFHDTLPHPEVAMDVAL
                     NQCLMDPALLTLERRTEDKASILVYSGQEAPPELAVEGKVFQVGRDRMLGAIGANRLD
                     RTAFLERLAASRVLVSYFGMTVLEAMFLGIPTVTYSIGSTVHDTLSALLEKETNVRFY
                     RKSQKAGQEIFDLTYTKRPSGQGFQILIDLIERYL"
     gene            2807..>3307
                     /locus_tag="PKK76_15600"
     CDS             2807..>3307
                     /locus_tag="PKK76_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69955.1"
                     /translation="MSFFQPRNIWLVIMVAAIGVSMYVREESKTPDIEVLKTKQISMM
                     VKMLASQALVVAKVPGPLTDGVKKQIKDQLGDIETKLPEREIFPVQKFIILSEIGESV
                     SLPDMGNKALIADLKLIYVDRTPAADNSPLFAIAGGDLARLKSLSLNKPKEAEALRAG
                     LEARASF"
BASE COUNT          780 a          793 c          872 g          862 t
ORIGIN      
        1 cgacggaacc attcaagtag cgggaagcac tccttttgaa atggcaaagg ccgcctattc
       61 tgaactggcg cgcacaactc cggtgagccc ggacgatagc ctcgctctct ttacgggtct
      121 gtgtcctttt ctcgacaaag gcattgcgga cgatctggcc aaagtgcaca ctcagtttta
      181 cgcgcactat tcgtattgcg aaaacagtcc ttatggattt ttgccggatc tggtttcatc
      241 tgagtttctg gcagaggcag agcccggcgc tacggatctg cgcgattttg tttttaagaa
      301 catagaaaaa tacgatgcag aaattctata ccgccggccg gatgttcgtc agtttcgtct
      361 ggatttttcg tgcggaagcc ccaggtccct cgatctttcg cgtcgcctgc gagccattga
      421 cccggatgta acatacgaaa ggatcgagaa ggtgatccag gatcatcctg aaatcattcg
      481 ttcatttcca tcatattttg agattgatct gaccagcctg agcaaggttc atcccatcct
      541 ttaccccgaa atctcaggta ctgattcttt gcctctggct ctggcgatta agctcaatgc
      601 agagattgca gctcatggct ggaaaaagga cgcaactgtg tctcttgcgg gtcgcggcga
      661 gcccatgctc catcctgatt ttttggaaat cctgaagttg tttttggcca atgaaagtgt
      721 cagcacagtc tttgtggaaa cgtacggtca ttccattgat gaaaagcaga taacgttatg
      781 gaattcgctg cccggagcgg aaaaactatc tgtcatactg cgcctttcca ctctgaaaaa
      841 agatcgatac aggtttctct atggagaaga tttgttcgag cctgcgatgg ctgctgtggt
      901 tcatcttgaa aaagcggcca atcgcaaatt cgcagcctat gtggaaatgc ttaagatcaa
      961 ggaagtggaa gatgagatca ctcctttctt tgatcgcttt gaaaactcga ccgttcgcgt
     1021 aatcttaaat aaatacaacc gttacataga tcttttgccg gagcgcagag tcacggatct
     1081 cactcctctg gacagaagtt tctgctggca catcgcgcgt gatttctact taaagccgga
     1141 cggaactgtg cctctgtgca aacaggacat tgttggccgc gctgcaaccc atgattttgc
     1201 aaagaaaacg attcttgaaa tcttatctgc cacggccagg gcacattcgc attctgtaag
     1261 aggcgagcac gattctgtag gaatgccctg cctgaactgt gacgagtggt acacgttcaa
     1321 cggatgaagt gcgcagtcct catccaggca agaagcgcat ccgtcaggct tcccggaaaa
     1381 atatacgagc ccgtgccaga atccggttca ccatctattc tggatcatgt ggtgcgccgt
     1441 atgcaacttg caaacgtggg cccggtggtg gtgctcattc cagaagatga tacgcttctg
     1501 gctgactatc taagcgtgcg gcggattgag tttcttcggg gcccgcatga ggacgttcgc
     1561 gcccgatatc gccatgcggc gcgagccatg aatctggatt tagttgtgcg cgccaccgca
     1621 gacaatccgg ctctcgatcc ggtctgtgcg gcgcgttccg ttcaggaaat tcagagtcgc
     1681 aatctggatc ttttttcttt ttctgccatg cctctgggca caggagtgga agtcttcacg
     1741 gctgccgccc tcttgtctga ttctctggaa ggacccgagt acagtgaaca tgtatctttg
     1801 cacatcaagc atgccccaga ccggtttcgc gtggagcact cgccatggag agaggggctg
     1861 cctccggaac ttccccgcct aaccgtggat acacgagagg atctggaagt ggttcgatcc
     1921 gtgtttcgca agctgggtgt ggactttact ctcgatcaac tcatggaact atttgcatcc
     1981 aatcccgaac tattcgcggg caatcgtcat gtggttcaga agacatttct gccgcctgga
     2041 gcatcgtctt gatcttgata ctggcaggtg cgggcgcccg atttgggacg ggccacgtag
     2101 agagaatgcg aattcttaaa ggtcttctcg aagcccgcgg tcattctgtg tctcttcgcc
     2161 tcacagatga gccagaagcg gagcgtggca gacatggaga ttcgctcatt gtttttgacg
     2221 cgcgcgattc tgcctgcgat gtgggggaac gcgtcatcgc agtggacaat cgttcctcta
     2281 ttcgcgcagg caacacgagc attgtgtttc atgacactct gcctcatcct gaggtggcta
     2341 tggacgttgc gctcaatcag tgtttgatgg atcctgcact tctgacgctg gaaagacgga
     2401 cggaagacaa agccagcatt ctcgtataca gtggccagga agcgccgccg gaacttgcag
     2461 tggaagggaa ggtttttcaa gtgggacgcg atagaatgct cggtgctata ggagctaaca
     2521 ggctggatcg cactgcgttt ctagagcgat tggctgcttc gcgagtactt gtatcttact
     2581 ttggaatgac tgttctcgag gctatgtttc ttggaattcc cacagttacg tattcaattg
     2641 gaagcactgt gcatgatacg ttatctgctc ttctcgaaaa agaaacaaac gtccgcttct
     2701 atcgaaagag ccagaaagcg ggtcaggaaa tttttgatct aacatatacg aagcgtccat
     2761 cggggcaagg attccagatt ttgattgatt tgatagagag gtatttatga gcttttttca
     2821 accgcgtaac atctggcttg tgattatggt ggcggccatt ggtgtgtcga tgtatgttcg
     2881 ggaggagtct aaaaccccgg acattgaagt tctcaaaaca aaacagatca gtatgatggt
     2941 caagatgctt gcatctcagg ctcttgttgt ggctaaagtg cccgggccat tgacggacgg
     3001 agtcaagaaa caaatcaaag accagttagg cgacattgag accaaactgc cagaaagaga
     3061 aatttttccg gtgcaaaagt tcatcattct ttctgaaata ggagagagcg tgtcgctgcc
     3121 ggatatggga aacaaagcac tcatcgctga tttgaaattg atttacgtgg atcgcactcc
     3181 tgcggcagac aattcgcctt tatttgccat tgctggcggg gatcttgctc gtttgaaatc
     3241 tttgtctttg aataaaccaa aagaagcgga agcgctgcgc gcgggcctgg aggcgcgcgc
     3301 gagtttc
//
LOCUS       DAORFF010000457         3299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_18727_length_3299_cov_3.381011, whole genome shotgun sequence.
ACCESSION   DAORFF010000457 DAORFF010000000
VERSION     DAORFF010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18727_length_3299_cov_3.381011"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PKK76_15605"
     CDS             complement(<1..410)
                     /locus_tag="PKK76_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69956.1"
                     /translation="MKQASIVLLYLFAACSTVQEVQVPSASRTEFPELAVAPSVRTGV
                     SDKREPGAHSSDGKLKFFLSRGAPLANVNRSMQETALLRVLRSIRFRDVDYVTLDDPM
                     GKPAALDGRLSLTFEAGKTTNEFTIAARLKEEEEG"
     gene            complement(438..854)
                     /locus_tag="PKK76_15610"
     CDS             complement(438..854)
                     /locus_tag="PKK76_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69957.1"
                     /translation="MIHAVGVKKIMAKAVNSKTTTKEPLSAEQMEIFFNLLTDYTDKV
                     SLINSVYESDLETAFQAMEGAVEEVLQWDTSSFSPDLRRTLLSYLTFHRETVWEIIQE
                     ARSLLMSDRREYVKRLVNHHKEFIKWLSRMERNFAA"
     gene            974..1927
                     /locus_tag="PKK76_15615"
     CDS             974..1927
                     /locus_tag="PKK76_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL69958.1"
                     /translation="MIVTLLGVRGSIATPLRSQEYQGKVRQILEKAIRENINESRIDS
                     FISELPPDLRYVIGGETTCCTVQIPGVPLPVIIDCGTALRHLGDELMAGPCGKGQGEL
                     HILVTHTHWDHIQGLPFFKPAYIPGNKLHFYSPLDDLQDRLVYQTTDRFFPMPFMATA
                     SEKHFHKLTGPIDLGGVQVDHHPLKHPGGCFAYKFSKDGHAFIFATDAEFTGTDLEHP
                     EEYKPFFYQADLVVMDAQYTLDESFAKFDWGHTSYTMAVNCAAQWKVKNLVLTHHEPA
                     YSDAKVFENYESAIEHRNIMRLEFPRVHLAREGLSFRIGPE"
     gene            1924..2364
                     /locus_tag="PKK76_15620"
     CDS             1924..2364
                     /locus_tag="PKK76_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69959.1"
                     /translation="MNRAFTLTAGFILFAAFGSVLPAIYSVYTRDLRAMDHAKGSQVF
                     VTLAKAQTRRSPTGDRFYYSIEFKWDGTKYRIPVEVTSEFYHLSKEGQNMEAQIFFDM
                     GRPYVRLPGNHIHPAGRPGLFYLGAAGLLAGILLLASSVLARSR"
     gene            2364..3152
                     /locus_tag="PKK76_15625"
     CDS             2364..3152
                     /locus_tag="PKK76_15625"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HNL69960.1"
                     /translation="MDLAISPCPNDTFIFCHLMRAMPELQLVFADVEELNQRALFEKK
                     HDLSKLSFYAILRTKGYTLLDCGGAMGQGCGPILLYGKKSNGKISDLKRILTPGKYTT
                     AALLLDLFLAENHPDPASVERVPVRYDKILPALGEEQAGVIIHEERFTYPSFGAVKAQ
                     DLGEWWEKSTGLPIPLGGIVIRDNLLNRKSEIESKIRDSLRAAWKDPESVKDFVKCYS
                     QSLEDSVIQSHIDLYVNRYTESLGEDGMRALDVLRQKAERAGLL"
BASE COUNT          805 a          841 c          810 g          843 t
ORIGIN      
        1 ccttcctctt cttctttcag gcgagcagca attgtgaatt cgtttgtggt cttgcctgct
       61 tcgaatgtaa gagagagtct tccatcgagt gcggcgggct tgcccattgg atcgtccaga
      121 gtgacgtagt ccacatcacg gaatcgaata gaacgcagaa cacgcagaag tgctgtctcc
      181 tgcatgcttc gattcacgtt tgcaagagga gctcctcttg ataggaagaa tttgagctta
      241 ccatccgaag aatgcgctcc gggttcgcgc ttgtctgaga ctccagtgcg cacagatggc
      301 gccactgcca gttcaggaaa ttcggtgcgc gaggcagaag ggacctgcac ctcctggacg
      361 gtggagcagg cagcgaaaag atatagaagt acgattgagg cttgtttcat attcgggctg
      421 cgcgcccgat ctatgcatca ggcggcgaag tttctctcca ttcgggacag ccactttatg
      481 aattccttgt gatggtttac aagccttttt acgtattccc tccgatcgga cataagaaga
      541 cttctggctt cctggataat ttcccagacg gtctcccggt ggaatgtcag atagctcagt
      601 agagtacgtc tgagatcagg agagaaggaa gatgtgtccc actggagcac ttcctccact
      661 gctccttcca tggcctggaa tgcagtctcc aaatccgact cgtacacact gttgataagg
      721 ctgaccttat ccgtgtaatc cgtcagtaag ttgaaaaata tttccatctg ctcggcagac
      781 aggggttctt tcgtagtggt ctttgagttc acggcttttg ccataatttt tttgacgcct
      841 acggcgtgaa tcatttattg tgctttttca gatcaaagtt ccagggccgc ggaagcggcg
      901 tgcagattcg gaaccgggat cgctggtcct accagcccct gaaagatgtc tgagactgcg
      961 gggtttcgcg ccaatgattg ttactctact tggagtacgg ggttccattg ccactccgtt
     1021 gcgctctcaa gaatatcagg gcaaagttcg ccagatactt gaaaaagcaa tccgcgaaaa
     1081 cataaacgag tcccgcattg attcgttcat ttccgaatta ccgccagacc ttcgatacgt
     1141 gatcggcgga gaaaccacat gctgcaccgt gcaaattcca ggagttccac ttcctgtcat
     1201 catcgattgt ggcactgctc tgcgtcatct tggtgacgaa ctgatggcgg gcccgtgtgg
     1261 gaaaggccag ggcgaacttc acattcttgt aactcacact cactgggatc atatccaggg
     1321 acttccattt ttcaagcctg cgtacattcc tggaaataaa ctgcatttct attctcccct
     1381 ggatgatttg caagatagac tcgtctatca gaccacagat cgatttttcc cgatgccatt
     1441 tatggccacc gcatctgaaa aacattttca caagctcaca ggacccattg acctgggcgg
     1501 agtgcaagtg gaccatcatc ctctcaagca tcctggcgga tgcttcgcgt acaaattttc
     1561 aaaagatggc cacgcattca ttttcgcaac agacgcggaa ttcacaggaa cagatctgga
     1621 gcaccccgaa gaatacaaac ccttcttcta tcaggcggat ctggtcgtca tggacgcgca
     1681 gtacactctg gatgagtctt tcgcaaaatt cgactggggc cacacaagct acacgatggc
     1741 agtcaattgt gctgctcagt ggaaagtgaa aaacctggtg ctcactcacc acgaaccagc
     1801 ctattcagac gcaaaagttt ttgagaatta cgaaagcgcc attgaacatc gcaacatcat
     1861 gcggcttgaa tttccgcgcg ttcaccttgc ccgggaagga ttgtccttcc gcattggccc
     1921 ggaatgaacc gtgctttcac tctgactgct ggattcattc tgtttgcggc gttcggaagc
     1981 gtccttcccg ccatttattc cgtgtataca agagatttgc gtgcgatgga tcatgcgaag
     2041 ggctctcagg tgtttgtgac tctcgcgaaa gcccagacaa ggcgcagccc tacgggagat
     2101 cgcttctact attccatcga attcaaatgg gacggaacaa aatacagaat tcctgtagaa
     2161 gtcacttccg agttttatca tctttccaaa gagggacaaa acatggaagc gcagatcttc
     2221 tttgatatgg ggcgccccta tgtgcgtctc ccgggcaatc acatccatcc cgcaggaaga
     2281 ccgggtctgt tctatctggg ggctgccggt ttattggctg gaattttgct ccttgcgtct
     2341 tctgttctgg cgaggtccag gtgatggatc ttgccatttc tccctgcccc aacgacactt
     2401 tcattttctg tcacttgatg cgtgccatgc cagagctgca gcttgtgttt gcagatgtag
     2461 aggaattgaa ccagcgcgca ctctttgaaa agaaacatga tctttctaaa ctatcgttct
     2521 atgcaattct ccgcacaaaa ggatacacat tgcttgattg cggtggtgcg atgggccagg
     2581 gatgcggacc cattcttttg tatggaaaga aatcaaacgg aaagatatct gacctcaaac
     2641 gaatcctgac tccgggcaaa tacacaacgg ctgctcttct tctcgatctg ttccttgctg
     2701 aaaatcatcc agaccctgcg tccgtagaga gagtccctgt aagatatgac aaaatactcc
     2761 cggccctcgg agaagaacag gcaggcgtga tcatccacga agaacgtttt acttatccat
     2821 cttttggcgc ggtcaaagcc caggatctgg gagaatggtg ggaaaaatcc acgggacttc
     2881 cgattccgct tggtggaatc gtgattcgag acaatcttct gaatcgcaaa tctgaaatag
     2941 aatcaaagat tcgagacagt ctgcgcgcag cgtggaagga tccagagtct gtcaaagact
     3001 tcgtgaaatg ctattcgcag tctctagaag attcagtaat ccaatctcac atagatctct
     3061 atgtgaaccg ttatacggaa tctctgggag aagacggaat gcgtgctctg gatgtgctaa
     3121 gacagaaggc ggagagagcc ggattgctct aacagctggc cgcatcatcc cgatttcgtg
     3181 cagcgccccg caagaccgtc atggaacatc gcctaattcc tgttacatcc cggaggaatt
     3241 tcatccgtcc ccccatccag gtttactaca cgaaacaaag gttgcgtttg ccgaggggt
//
LOCUS       DAORFF010000458         3289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_18808_length_3289_cov_4.060297, whole genome shotgun sequence.
ACCESSION   DAORFF010000458 DAORFF010000000
VERSION     DAORFF010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18808_length_3289_cov_4.060297"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2033)
                     /locus_tag="PKK76_15630"
     CDS             complement(<1..2033)
                     /locus_tag="PKK76_15630"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNL69961.1"
                     /translation="MIELEYHGKVRIDRDQNGIPHITADTESGMYFGQGYAQAMDRGR
                     QILLMRILSQGRASEILESSDEMLEIDRFFRRMNWYGDSAQIAGQLDAATSKLAQAFC
                     DGINAFLSKKLPFEFRFLIGLKYELWEIDDSITLARMIGYISLQQSQTEVERLFVQMV
                     QAGIDRKLLEEFFPGSLDHLDEKVIRQVKLSERVVPAALQWSRIIPKVIASNNWVVAG
                     SRTKSGKPILSNDPHLEINRLPNVWQEIILTFAGRTACGVSMPGLPGILIGRNPDVSW
                     GATYTFMDAVDSWVEECRQGSYKRSQFLRVQWVRFKERREIIKRKKKPDHLEIFYENE
                     HGVLDGDPGEEGFYLATRWASGSGTGARSLEAILSMFHVTGVKQAMDTMGRLEMSFNW
                     VLADKQGNIGYQMSGLMPKRQKNWSGLPPMPGWDSDFDWKGFHSHMDLPRRYNPKEGY
                     IVTANNDLNAWGNAAPINAPMGSYRADRIEKLLKRSELTVEDMQKMHMDVYSLQAERY
                     MEILRPTLPASNPNAEILRGWDLRYDLDSKGAYIFEKFYEFLLEEVFGSVFGKQVWTH
                     IRTETGILTDFFGNFDRILLSRKSKWFGSKSRDDLFSAALTRALSEEVKAYGSGQNLI
                     LKHIILGGKFPLFFRIDRGPVGLPGNRATIHQGQIYRSAGRDTSFAPSYRMVA"
     gene            2134..2364
                     /locus_tag="PKK76_15635"
     CDS             2134..2364
                     /locus_tag="PKK76_15635"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNL69962.1"
                     /translation="MHFSGTKIRALREERKWTQKELAEKIALTGHFSGFTHQRIDRLE
                     KGDNASHRVLCALCEVFRVRPGVFFEDGDSAT"
     gene            2366..>3289
                     /locus_tag="PKK76_15640"
     CDS             2366..>3289
                     /locus_tag="PKK76_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69963.1"
                     /translation="MIPHTFSILEAGEKLVVDLPWCRSNPEEVHEKTRILTQDMQYRL
                     TFDGKIIKEGKVGRDFRIEVDLRGQKSGVYTMQVMGNGTTDGGVREFWKQHIFVQTSS
                     RKTPEQIREIANRYAPIFLFSKKEQYFPVALGDLIHSPELLKSNGKISVDSIAGELHV
                     PITELDEFLRHNGHADYLLNQSVFDLDGVFKEIHGDFRRSVVYYSYIEDKNSDRFFIN
                     YHTFYAFDPKTGIAKMLNIGPHVFDRESLTMVFRGDGTPESLVLSGHLENQPILFLEK
                     LMIWSTGRVRIPAPDPYGPSVDTHPIVPVAEG"
BASE COUNT          870 a          842 c          759 g          818 t
ORIGIN      
        1 tctgccacca tacgatagct gggcgcaaag cttgtatctc tacctgcact gcgatagatc
       61 tgtccctgat gaatggtggc gcgatttccg ggaagaccca ccggcccgcg atcgattcta
      121 aaaaacagag ggaattttcc gcccagaatg atgtgtttta agatgagatt ttgtcctgag
      181 ccgtaggcct tcacttcttc tgaaagagca cgcgtgagtg ccgcggagaa taagtcgtct
      241 cgcgactttg atccaaacca tttggatttg cgcgataaaa gaattctgtc gaagttccca
      301 aagaagtcag tgagtatccc tgtctctgtg cggatatggg tccagacctg tttaccaaaa
      361 acggaaccaa acacttcttc gagcaagaat tcataaaact tctcaaatat ataggcacct
      421 ttagaatcca gatcatacct cagatcccaa ccgcgcaaga tctccgcatt cggattggac
      481 gcgggaagag taggcctcag gatttccata tatctctctg cctggagaga atacacatcc
      541 atatgcattt tctgcatgtc ttctacagtg agttcagacc gtttgagtag tttttcgatt
      601 cgatccgcgc gatagcttcc catgggagca ttgatgggcg ctgcatttcc ccacgcattg
      661 agatcattgt ttgccgttac aatgtatcct tcttttggat tgtaacgacg aggcaaatcc
      721 atgtgagagt gaaagccttt ccagtcaaaa tcagaatccc agccaggcat gggaggaaga
      781 ccggaccagt tcttctgtct tttgggcatg agaccggaca tctggtaacc aatgtttccc
      841 tgcttgtcag ctaacaccca gttaaacgac atttccaacc tgcccatcgt gtccatggcc
      901 tgctttactc cggtcacgtg aaacatagaa agaattgctt cgagcgatct tgctcctgtt
      961 ccagaacctg aggcccagcg agtcgccaga tagaaacctt cttctcctgg atcgccatcg
     1021 agaactccat gctcgttctc atagaagatt tcgagatgat cgggtttctt ctttcttttg
     1081 atgatttcgc ggcgctcttt gaatctcacc cactgcacgc gcaaaaattg actgcgctta
     1141 tagcttccct gtctgcattc ttctacccag gaatccaccg cgtccatgaa agtgtacgtc
     1201 gcgccccacg acacatccgg attgcggcca atcaaaatcc cgggaagacc tggcatcgaa
     1261 acgccgcatg cagttctacc tgcgaacgtc agtatgatct cttgccacac attcggaagg
     1321 cgattgattt caagatgagg atcattgctg agaatcggtt ttccggattt agtgcgagaa
     1381 ccagccacga cccagttgtt ggatgcgatt acctttggaa tgatgcgact ccactgaagc
     1441 gcggccggaa caactcgctc tgacaatttc acctgacgga ttactttctc atccagatga
     1501 tccaggcttc ctggaaaaaa ttcttccaga agcttgcgat cgattccagc ctgaaccatt
     1561 tgaacaaaca atcgttctac ttctgtctgc gactgctgaa gactgatgta accgatcata
     1621 cgagccagag tgatcgaatc atcgatttcc cagagttcat acttcaaacc aatcaagaat
     1681 cgaaattcaa atgggagttt cttagaaaga aatgcattga tcccgtcgca aaaagcctgc
     1741 gcgagtttag atgtggccgc gtccagctgc cctgcaatct gagcgctgtc accataccag
     1801 ttcatcctcc gaaagaaacg atcaatctca agcatctcat ccgaggattc gagtatctcg
     1861 gaagcgcgac cctgagaaag aatgcgcatc agtagaatct gccttccgcg atccattgcc
     1921 tgagcgtagc cctggccaaa atacatgccg ctttccgtgt ctgctgttat gtgaggaatt
     1981 ccgttctgat cccggtcaat cctgactttt ccgtgatatt ctagttcgat catgtctttg
     2041 gctccgaatc ccagcaggcg gaacgatccg caaatttgcg agaaaatttc tctttacaat
     2101 ttcgtcaaaa tcactcaact tcagacgggg gatatgcact tcagcggaac aaaaatccga
     2161 gccctgcgcg aggagcgaaa atggactcag aaagaactcg cagagaagat tgcactcacc
     2221 ggacatttta gtggcttcac acaccagaga atagacagac tcgaaaaagg agacaatgcc
     2281 tcccatcgag tgctgtgtgc gctgtgcgaa gtctttagag tgcggccagg tgtattcttc
     2341 gaagacggag attctgcgac ataatatgat tcctcacaca ttcagcatct tagaagcagg
     2401 cgaaaagctt gtggtggacc tgccgtggtg cagaagcaat ccggaagagg ttcatgagaa
     2461 aactcgcatc ctcacacagg atatgcaata tcgcctaacg tttgatggaa agatcatcaa
     2521 agaaggcaaa gtcggacgcg attttcgcat agaggtggat ctgcgcggtc aaaagagcgg
     2581 cgtctatacc atgcaagtca tgggaaacgg aaccacagac ggaggagtcc gcgaattctg
     2641 gaaacagcat atatttgttc aaacatcatc gcgcaaaaca ccggaacaaa ttcgggaaat
     2701 cgcaaaccgt tatgcgccca tctttttgtt cagcaagaaa gagcagtatt ttccggttgc
     2761 actcggagat ctcatccact cccctgagct tctcaagtcc aatggaaaga tttctgtaga
     2821 ttctatcgca ggggaactgc atgtccccat cacagagcta gacgaatttc tgcgacacaa
     2881 cgggcacgca gactatctgt tgaatcaatc cgttttcgat ttagatgggg tgttcaaaga
     2941 aatccacgga gacttcaggc gatccgttgt ctattactcc tacatagaag acaaaaactc
     3001 agacagattc ttcatcaact accacacgtt ctacgcattt gatccaaaga cgggcattgc
     3061 aaagatgctc aacattgggc cgcatgtctt tgaccgagaa agtcttacca tggtgtttcg
     3121 cggcgacgga acgcctgagt ctctggttct atcagggcat ctcgaaaatc agcccatcct
     3181 gtttctggaa aagctgatga tctggagcac tggccgggtg cgcatcccgg cgccggatcc
     3241 ttacggtccc tcagtggata ctcatccgat tgtgccggtt gcagaagga
//
LOCUS       DAORFF010000459         3289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_18811_length_3289_cov_3.132962, whole genome shotgun sequence.
ACCESSION   DAORFF010000459 DAORFF010000000
VERSION     DAORFF010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18811_length_3289_cov_3.132962"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PKK76_15645"
     CDS             complement(<1..518)
                     /locus_tag="PKK76_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNL69964.1"
                     /translation="MAIGFQTDRVSEINPIQTRDTSPRDLICEICRNFYTLGWVSGTG
                     GGVSIRRNDTIYMAPSGVEKEKIKPEDIFELDLAGNVTRHAKNEKLKLTECAPLFLSA
                     FEMRNAGAVLHSHSPNVVLSTLYAKRVEGRISVLEFTELEMIKGLEGHGYHDVFTLPV
                     VDNTAKECDLTDS"
     gene            618..1244
                     /locus_tag="PKK76_15650"
     CDS             618..1244
                     /locus_tag="PKK76_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69965.1"
                     /translation="MHNTEATAQALNILRRGDQFQWSVIPFLAFVCYVYFTEIKNRNW
                     ASVSAGLALYGVHWFYEILNALIQHATGHALWTVPTGTSFLIFIGVGIEISLMFAVSG
                     IIISRLLPENPSEKIAGLPLKWLHVISFAVLYSVIEIFLVKATPVFVWVYDWWGAIPV
                     GLTVYVPFFAAAVFAYYWQPRTRLIFISAVWILDLFLVLVFAVVLRWI"
     gene            complement(1271..2143)
                     /locus_tag="PKK76_15655"
     CDS             complement(1271..2143)
                     /locus_tag="PKK76_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69966.1"
                     /translation="MKSRMIQTACLILLPFASFAEKSHEQMGDAYYKKKDFGLARIEY
                     DRAILDSSNESVRAKIGLTLIRERRFRQAIESLQGKSFDIMYFRMYGDLRANFRNRFL
                     MDQGELEESTLPESKKDLGRLLAGTLYLEDGDFDRARIHFEKLQRETENERVRIAASE
                     TLTAMTTYNEIPRKSVWLAAGLSALLPGSGQVYSRNVADGLTAFAFTSVLCGSAAYMN
                     HLETRSRSGHGGSIIMGILGLGFYLTNITGAAASAGRFNTFQERKFQQTIRDRFFNID
                     FVEQTSDIRFETSF"
     gene            2196..2504
                     /gene="yidD"
                     /locus_tag="PKK76_15660"
     CDS             2196..2504
                     /gene="yidD"
                     /locus_tag="PKK76_15660"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNL69967.1"
                     /translation="MFRPMLYLIDRHREEFTHYDGPRCPMYPSCAAYADRAIRQYGFL
                     GALMFANRLFFAESGDLASKYVLVPHRMSEMPRYYDPVSDDILPGEHRPSLLMEDFSE
                     "
     gene            2545..>3289
                     /locus_tag="PKK76_15665"
     CDS             2545..>3289
                     /locus_tag="PKK76_15665"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNL69968.1"
                     /translation="MGILKTLAPLLGHPDYSGARDPFYFSRAIRSLRSPLDLPDMEKG
                     IAMLRSVIEAQGHVLVVGDRDVDGVSSAALLANFLRDQHAKRGGGLEIRVSDLGDDYG
                     LSGELFENVRDGKADLVILLDMGSSHGPEIHELIAAGKKVIVLDHHQVSDRRPSHDLC
                     AFINPQLNAEVHENEGKIPTVGLAFKFLFGYALSHIAEWNNHSLVDLSFLFSENANAG
                     ARFAVYRCGCLVESGENISGAHLRPLDADG"
BASE COUNT          734 a          799 c          826 g          930 t
ORIGIN      
        1 gaatctgtga ggtcacattc ctttgcagtg ttgtctacga ccgggagagt aaatacatcg
       61 tggtaaccgt gaccttccag gcctttgatc atctccagtt ccgtgaactc gagaactgaa
      121 attcgtcctt ccactctttt tgcatacagt gtggacagca ctacgtttgg cgaatgcgaa
      181 tgaagcaccg cgcctgcgtt tcgcatttca aaagcagaaa ggaatagagg cgcgcattca
      241 gtgagtttga gtttttcgtt ttttgcgtgg cgcgttacat ttccagccag atccagttca
      301 aagatatctt ccggtttgat tttctctttt tcaacgccac tgggcgccat gtagattgta
      361 tcattgcgtc gaatggaaac cccaccgccc gttccactga cccagccaag agtgtaaaaa
      421 ttgcggcaga tctcgcagat caaatctctc ggggaagtat ctctagtctg gatggggttg
      481 atttcgctga cgcgatctgt ctgaaatcca atggccatgg cgaccagata aagggaagtg
      541 aggctgttcg gccagatgaa aaaatctccc tggatcgata ttgcgcgaaa acgcagcgcg
      601 caccctgatg gcattctatg cacaacacgg aagcaaccgc gcaggcgttg aatattcttc
      661 ggcgcggtga tcagtttcaa tggagcgtca tcccgtttct ggcatttgta tgctatgttt
      721 atttcactga gatcaagaat cgaaactggg cttccgtctc ggctggtctt gccctgtacg
      781 gagtgcactg gttttacgaa atcctaaatg ctctcatcca gcatgcaact ggacatgctc
      841 tctggactgt gccgacaggt acttcgtttt tgattttcat tggagtcggg attgaaatct
      901 ctttgatgtt cgctgtttcc gggatcatca tttccaggct ccttccagaa aacccgtctg
      961 agaaaatagc cggccttcca ctcaaatggt tgcacgtgat ttcttttgcc gtactctatt
     1021 cagtcattga aatcttcctg gtcaaggcca cccccgtatt tgtctgggtg tatgattggt
     1081 ggggcgcgat cccggttggg cttactgtat atgtgccatt ttttgcagcg gcagtattcg
     1141 cgtactactg gcagccccgc acaagactga tcttcatcag cgcagtgtgg attctggatt
     1201 tgttcctggt tcttgttttc gcagtagtcc ttcgctggat ctagtggtgc gcactcaatg
     1261 acgatttaaa tcagaaactg gtttcgaacc gaatgtctga ggtttgttcc acgaaatcga
     1321 tattgaagaa cctgtcacga atcgtttgtt gaaatttgcg ttcctgaaaa gtgttgaatc
     1381 tacctgcgga agcagcagcg cctgtgatgt tggttagata gaaacccaga ccaagaattc
     1441 ccatgatgat ggaaccgcca tgtccggaac gagagcgcgt ttccagatga ttcatgtatg
     1501 ccgccgatcc gcaaagtacg ctcgtgaatg cgaaagcggt gagaccatct gcaacgttgc
     1561 gcgagtaaac ctggcccgat cccggaagca aggcggaaag cccggctgca agccatacgc
     1621 ttttgcgcgg gatttcattg taggtggtca tggctgtgag cgtttcgctt gcggcgattc
     1681 tcactctttc attttccgtt tcgcgctgga gtttttcgaa atgaattcgg gcacgatcaa
     1741 agtcaccgtc ttccaggtat agagttcccg ccaggagtct ccccagatct ttctttgact
     1801 cagggagcgt gctttcttct agttctccct ggtccatgag aaagcgattt cgaaagttcg
     1861 ctcttaaatc gccatacatg cgaaagtaca ttatatcaaa tgattttccc tgaagagatt
     1921 caattgcctg gcgaaacctt ctttcgcgaa ttagggtcag gccaattttt gcgcggactg
     1981 actcattgct tgaatcgagt atcgcgcggt cgtattcgat tctggccagt ccgaagtctt
     2041 tctttttgta gtacgcatct cccatttgtt cgtgactttt ctcagcgaag cttgcaaagg
     2101 gtagaagaat gaggcacgca gtctggatca ttctgctttt catgacagtt tgcaaatcgg
     2161 cacagagtcc cgcggccggc gaggaaaatc cgatcgtgtt tcgtcctatg ctgtatttga
     2221 ttgatcgcca ccgcgaagaa ttcactcact atgacggtcc acgttgtccc atgtatcctt
     2281 cgtgcgcggc ctatgcggat cgggccattc gccagtatgg ctttctgggc gccctgatgt
     2341 ttgcaaacag gcttttcttc gcagaaagcg gagatctagc atccaaatac gtgcttgtgc
     2401 ctcatagaat gtctgaaatg cctcgatact acgatccagt gtccgatgac atccttccag
     2461 gagagcacag gccgtctctt cttatggaag acttctcaga atagttgacc ggattctgtc
     2521 tcatagacat aatacccttc agtcatggga atactcaaaa cactggctcc gcttctgggc
     2581 caccctgact actcgggcgc ccgcgatcct ttttactttt ctcgcgccat tcgttctctg
     2641 cgttcccctc tggatttgcc ggatatggag aagggaatcg ctatgcttcg atccgtcatc
     2701 gaggctcagg ggcatgttct tgttgtggga gacagagacg tggacggcgt gagttctgcc
     2761 gcacttcttg cgaatttcct gcgagaccag catgcgaaaa gaggaggcgg acttgagatt
     2821 cgtgtctctg atctgggaga cgactacgga ttatcgggag agctttttga gaatgtgcgc
     2881 gatggaaaag cggacctcgt gattttgctc gatatgggtt cttcacacgg gccagagatt
     2941 catgagctca ttgctgccgg aaagaaagta attgttctcg atcaccatca ggtttccgat
     3001 cgcaggcctt cgcatgatct ttgcgcgttc atcaatccac aattgaatgc agaagttcac
     3061 gaaaatgaag gcaagattcc aaccgtcggc cttgctttta agtttctatt cggctacgca
     3121 ctttctcaca tcgcagagtg gaacaatcat tctcttgtag atttgtcgtt tttgttttca
     3181 gaaaatgcga atgcaggcgc caggtttgca gtctatagat gcggatgcct tgtggaaagc
     3241 ggggagaata tttctggagc acaccttcgt ccactcgacg cggatggaa
//
LOCUS       DAORFF010000460         3256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_19134_length_3256_cov_3.810059, whole genome shotgun sequence.
ACCESSION   DAORFF010000460 DAORFF010000000
VERSION     DAORFF010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19134_length_3256_cov_3.810059"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /gene="purE"
                     /locus_tag="PKK76_15670"
     CDS             complement(<1..430)
                     /gene="purE"
                     /locus_tag="PKK76_15670"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNL69969.1"
                     /translation="MNQPVVSIIMGSASDRSVMEQAAVALKEFSIPYEMTIVSAHRTP
                     DRMVEFAASARKRGIRVIIAGAGGAAHLPGMVASLTELPVIGVPILSSNSLDGWDSLL
                     SIVQMPAGVPVAAVAVNGAKNAGVLAARILGAGDPDLLDRM"
     gene            complement(427..1530)
                     /locus_tag="PKK76_15675"
     CDS             complement(427..1530)
                     /locus_tag="PKK76_15675"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNL69970.1"
                     /translation="MIGILGGGQLGRFLVNAAREWDIECAVLDPDPEAPAGKIAARFE
                     TGNLTDSSRVLDFARGLDLLTVEIENVSADALEEIATRIRVHPSASVLRIVQDKGIQK
                     DFYRRNNIPTAPYRLISSREELPDSESSYPLIVKQRRGGYDGRGVKKINSPADRSEAF
                     AEPSVQEECISIDQEISVIVSRNEFGQMAAFPAVEMVPTPAHVLDYLLSPARITEDQH
                     RRANEIAFEIAELIGLVGILAVEMFISNDQVLVNEIAPRPHNSGHATIEANMTSQYEQ
                     HLRMILGHPPGPTDSLSSSALLNLLGQGDGTPLIHGMREAMGIPGVSVHMYGKRVCKP
                     GRKMGHVTVLAPDRSILLERIELVRSVLRVGPQ"
     gene            complement(1527..2435)
                     /locus_tag="PKK76_15680"
     CDS             complement(1527..2435)
                     /locus_tag="PKK76_15680"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL69971.1"
                     /translation="MNEIKVLYDGALALERKGKPDDAIKYYTEIIKLDPGFRAAYNNL
                     GAIYARKGRQDLSIANFEKALSLGEDEAVHFNLGTEYFRREELEKARHHLKAALRIHP
                     RMLRAHIVLAYLYEKAGAPEKAEVYFKNALVVEPSNRMAALGLAVSLTGRGEEAAALQ
                     IVRSYLKEKPGDLTFENLQASLLLATGQVEQSRQEFLKLAKTSAEFTSFTDHVQKARS
                     EKDEEFSAMFEGMDLKIQSRTRRVLETRKKQAAADPTEKKQEVKDLMDLSFLYLFKGD
                     SDKALQLLFQAKKLGEETQGGEKPAS"
     gene            complement(2432..>3256)
                     /gene="nadC"
                     /locus_tag="PKK76_15685"
     CDS             complement(2432..>3256)
                     /gene="nadC"
                     /locus_tag="PKK76_15685"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNL69972.1"
                     /translation="EDAPAGDPTSEDIFTPQMRTTARLVARESGVLCGMPLVLRLLDL
                     FEKEAGYKIELVSSDKDGDHFERGSVLLTLKGPVVGILRLERLLLNFLQYLSGIATTT
                     SDAVRSAPPGVFILDTRKTLPGYRRLAKYAVYTGGGTNHRIHLSEMAMIKDNHIAAAG
                     GIRSAVDSIRAKHPQLPIEIEVDRVEQIPEALSLSPRVILLDNMTADTVRAAVAKIAS
                     ICKTSGAMPPIVEVSGGWTPSRISELNSMELPVGVSMGFLTHTTRFLDLSLEVDPA"
BASE COUNT          773 a          878 c          790 g          815 t
ORIGIN      
        1 gcatccggtc gagtaaatca ggatcacccg cgccaagaat acgagccgca agaacgcctg
       61 cattctttgc tccgttcact gcaacagctg ccactggaac gcccgctggc atttgcacaa
      121 tagatagtaa agaatcccaa ccatccagtg aattggagga aaggatgggc acgccaatca
      181 cgggtagctc tgtcagagac gcgaccattc ctggcaaatg tgcggctccg cccgctccgg
      241 ctatgatcac gcgaatccct ctctttcgcg cagaggcagc gaattcgacc attcgatctg
      301 gagtgcgatg agcagagacg atggtcattt cgtaaggaat agaaaactct ttgagcgcga
      361 cggcggcctg ttccatgaca gaacgatccg atgcactgcc catgatgata gaaactactg
      421 gctgattcat tgcggcccca cacggagcac actgcgaacc aattcgattc tctcaagcaa
      481 aatagatcga tctggagcaa gaaccgtaac gtgccccatc tttcttcccg gtttgcacac
      541 tcgtttgcca tacatgtgca cggacacacc tggaatgccc atcgcttctc gcattccatg
      601 tatgagaggc gttccgtctc cctgaccgag cagattcaaa agagcactcg aagacaggga
      661 atcggtagga cctggcggat gacccaggat catgcgcaga tgttgctcgt actgcgaagt
      721 catgtttgct tctatggtcg catggccgct gttgtgagga cgcggcgcga tttcattgac
      781 cagcacttga tcattggaaa taaacatctc cactgcaaga attccgacaa gtccaatgag
      841 ttccgcaatt tcaaaggcga tctcattggc gcgtctgtgt tgatcttctg tgattcgagc
      901 tggactgagt aaatagtcga gcacgtgtgc tggagtggga accatttcca cggctggaaa
      961 agctgccatc tgcccaaact cattgcgact gacgatgaca gaaatttcct gatcaatgct
     1021 gatgcattcc tcctgaacgg aaggttctgc aaatgcttcc gatctatctg caggcgaatt
     1081 gattttcttc acgccgcgcc catcgtatcc acctcttctc tgtttgacaa tcaacggata
     1141 agaagattca gaatcgggca attcctctct ggaggaaatc aggcgatagg gtgcagttgg
     1201 aatgttattt cttctataga aatctttctg aatgcctttg tcctgaacga tccgaagaac
     1261 agacgccgac ggatgaactc gaatgcgtgt tgctatttct tccagagcat ctgcacttac
     1321 gttttctatc tcaacggtaa gtagatccag gccgcgcgcg aaatccagga cccgcgaaga
     1381 gtcggtgagg tttccagttt caaatcgagc tgcaattttt ccggcaggtg cttccgggtc
     1441 cgggtcaagc acggcacatt cgatgtccca ctctcgcgct gcgttgacca ggaatctgcc
     1501 aagctgtcct cctccgagga ttccaatcat gacgcaggtt tttctccgcc ctgtgtttct
     1561 tcgcctaatt tttttgcctg aaacaatagc tgcagagctt tgtccgaatc acctttaaac
     1621 agatagagaa aactcaaatc catgagatcc ttcacttcct gcttcttctc agttggatcc
     1681 gcggccgcct gcttctttct ggtttccagt acgcggcgag tccgggattg aattttcaga
     1741 tccatccctt cgaacatggc gctgaactct tcatcttttt ccgaacgcgc tttctgcaca
     1801 tgatccgtga aagaagtaaa ctccgcagat gtcttcgcaa gcttgagaaa ctcctgcctg
     1861 gattgctcta cttgccctgt tgcaagaagg agagaggcct gaagattctc gaaagttaga
     1921 tcccctggtt tttcctttag ataagagcgc acaatctgga gcgctgctgc ttcctcgcct
     1981 cgaccggtca gactcaccgc cagaccaagt gctgccatgc gattggaagg ttccacgacc
     2041 agggcatttt tgaaatagac ttccgccttc tccggcgcgc ccgctttctc ataaagataa
     2101 gccagtacaa tatgcgcgcg cagcatgcga ggatgaatcc tgagtgccgc cttcaggtga
     2161 tgccgagctt tctccaactc ttcgcgacga aagtactccg tgcccagatt gaaatgaact
     2221 gcctcatctt ctcccaggga caacgccttt tcgaaattgg cgatggacag atcctgtctg
     2281 ccctttcgcg cgtagatcgc accgaggttg ttgtaggccg cgcgaaagcc aggatctaac
     2341 ttgatgattt ccgtgtagta cttgatggca tcgtctggct ttcctttcct ttccagagca
     2401 agcgcgccat cgtagagaac tttgatttcg ttcatgcagg atccacttcg agactgagat
     2461 ccagaaatcg ggttgtatga gtgagaaatc ccatgctaac tccaaccgga agttccatag
     2521 aattaagttc actgattcga gaaggcgtcc atccacccga aacttcaacg ataggcggca
     2581 ttgctccgga tgttttacag atagacgcaa ttttcgcaac agcggcccga accgtatccg
     2641 cagtcatgtt atcgagcaga atgactctcg gagaaagtga aagagcttcc gggatttgct
     2701 ccaccctatc cacttcgatt tcaataggaa gctgtggatg cttcgcacga atcgaatcga
     2761 ccgcacttcg gattccaccg gcggctgcaa tgtgattgtc tttgatcatt gccatttcgc
     2821 tgagatgaat tctgtgattt gtgccgccgc cggtgtagac tgcgtacttt gcgaggcgtc
     2881 tatatcctgg cagagtcttc ctggtatcga gtataaaaac tccgggagga gcacttcgca
     2941 cagcgtcgct cgtagtagtt gcaatgccgc tcaggtattg aagaaaattc aatagaagtc
     3001 gctccagtct aagaatgccc accacgggac ctttcaaagt caaaagaacc gaacctcttt
     3061 caaaatgatc cccatccttg tcggagctta cgagttctat cttgtatcct gcttccttct
     3121 caaagagatc cagaagacgc aggaccagag gcatgccgca cagcactccg gactctctgg
     3181 caacgaggcg agcggtcgtg cgcatttgcg gcgtaaagat gtcttctgac gtgggatcac
     3241 cggccggcgc gtcttc
//
LOCUS       DAORFF010000461         3253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_19163_length_3253_cov_3.623827, whole genome shotgun sequence.
ACCESSION   DAORFF010000461 DAORFF010000000
VERSION     DAORFF010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19163_length_3253_cov_3.623827"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /gene="ispG"
                     /locus_tag="PKK76_15690"
     CDS             complement(<1..904)
                     /gene="ispG"
                     /locus_tag="PKK76_15690"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HNL69973.1"
                     /translation="MQNIVQPAEAGLIPETFADSPFAYSRFRTREVKVGNVGVGGTNP
                     IRIQSMTISDTMDTEATVKEAIGLIEAGCEIVRITAPRVKEAENLKNIKNRLVDLGYN
                     QPLVADIHFLPQAAMTAVEYVEKVRINPGNFADRKFFELREYTDSQYEEELERIHDAF
                     RPLVLRARELGRALRIGTNHGSLSDRIMNRYGDTALGMVESALEFIRIAEHYDFRDII
                     ISMKASNPGVMIQAYRLLVARFRRENMDYPLHLGVTEAGGGKDGRIKSSIGIGSLLDD
                     GIGDTIRVSLTEDSIHEIPVARKIA"
     gene            962..2440
                     /locus_tag="PKK76_15695"
     CDS             962..2440
                     /locus_tag="PKK76_15695"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNL69974.1"
                     /translation="MARRIKPIDQLLSETEKEALHRNLGPFSLIALGVGAIIGAGIFI
                     LTGNAAAGENLSTTSLLHAPVLDIIMGGLDIQSHGRLPAGPGISVSFLITGLICALAA
                     LNYAELASMIPVAGSTYTYAYATLGELIAWIIGWDLILEYAVSNMAVAVGFSAYFNDV
                     LESIFGIRLPPELSRPLIENGAMTGAWFNLPSFIILMILTILLVRGVRQSAHTNNLMV
                     LIKLGAIVLFIIGGAKYIRFEHYHPFFPGGFSGVMAGAAIVFFTYFGFDSVSTAAEES
                     RNPARDMPIGIIASLIVCALLYVSVAIVLTGIADYRTLNNPAPVAGALKALGLDSIRS
                     LVGTGAVVGIISCLLVFQYGQARIWYAMSRDGLLPAAFSRVHPKFRTPHISTWIAGLV
                     VGIPAGIWDIGTFADLANIGTLAAFVIVSAGVLILRRTEPDRKRGFRVPFVPWLPILS
                     IVTCAFLMISLPLETWIRFFVWMAIGLVIYVLYGRHRSSMAQ"
     gene            2499..3113
                     /locus_tag="PKK76_15700"
     CDS             2499..3113
                     /locus_tag="PKK76_15700"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNL69975.1"
                     /translation="MARKENASVIQTASAKRPPLTKKLILDAALKIIETEGMEKLSMR
                     RIAALLDCSVAGPYVYFTNQEEILKALIDDGEARLTAELKAAVQTKVDIFDKIFILMQ
                     TYKSFASANRELHKLMFAMGGRGKSFSLISPSYRVYFETVRQGAKNGAIRCDRAGYHA
                     IARAMWSWGYGLVVLELARHPETKKSEIDPIAEGMELFKKLLQP"
BASE COUNT          740 a          859 c          817 g          837 t
ORIGIN      
        1 aagcaatctt cctcgcgact ggaatttcgt ggatcgagtc ttccgtcaaa gaaacgcgga
       61 tcgtatcccc gattccgtca tcgagcaagc ttccaatccc aatgctggat ttgattcttc
      121 cgtcttttcc tccacctgct tccgtcactc ccagatgaag tggatagtcc atgttctccc
      181 tgcgaaaacg cgccacaagc aggcgatatg cctggatcat cacccctgga ttggacgctt
      241 tcatagagat gatgatatcg cgaaaatcat agtgctccgc aattcgaatg aattcgagtg
      301 cggactcaac cattccaagg gcggtgtctc cgtatcgatt catgattctg tcagaaagag
      361 atccgtgatt cgtgccaatc ctcagagcgc ggcccagttc gcgcgcacgc agcacaagcg
      421 gccgaaacgc gtcgtgtatg cgctcaagct cttcttcgta ctgcgaatct gtgtattcgc
      481 gcagctcaaa gaattttcta tctgcgaagt tcccaggatt gatgcgaact ttctcaacgt
      541 actcaaccgc agtcatggct gcctgaggga gaaagtgaat gtccgcaacc agaggctggt
      601 tgtaacccag atcaaccagg cgattcttaa tattcttcaa gttttccgct tctttgacac
      661 gtggagcagt gatcctgacg atttcgcagc cagcttcgat caggccgatc gcttctttga
      721 ccgtggcttc cgtgtccatg gtatcagaaa tcgtcatgga ctggattcga attgggtttg
      781 tgccaccgac gcccacgttt ccaactttca cttcgcgcgt gcgaaaccgc gaataagcaa
      841 aaggagaatc tgcaaatgtt tcggggataa gtccggcttc tgcgggctgc actatattct
      901 gcatactaca cgaatgccaa atttgctttg acaggcaatc atttcaggac cagtgaactg
      961 catggcccgt cgcatcaaac caatcgatca gctgctttca gagacggaga aagaggctct
     1021 gcaccgaaac ctgggcccat ttagcctgat cgccctggga gtgggagcca tcattggagc
     1081 ggggattttt atcctgactg gaaatgcagc agccggggaa aatctgagca caacctccct
     1141 tttgcacgct cctgttctgg acatcatcat gggcggactg gacatacagt cccacggaag
     1201 acttccggcg ggacccggga tttcagtcag cttcctcatc accggactca tttgcgctct
     1261 ggctgcactc aactacgcgg aactggcctc catgattccc gtggctggca gcacatacac
     1321 ctacgcctac gcgacgcttg gtgaactgat cgcctggatc attggatggg atctgatcct
     1381 ggaatacgct gtatccaata tggctgttgc ggttggtttc tccgcttact tcaacgatgt
     1441 tctggagtcg atttttggaa ttcgtctccc gcctgaactt tcgcgaccac tcattgaaaa
     1501 cggcgcgatg acgggagcgt ggttcaatct gccttcgttt atcatactga tgattctgac
     1561 gattctgctg gttcgcggcg ttcgccagag cgcacataca aacaatctca tggttctcat
     1621 caagctcgga gccatcgtgt tgttcatcat tggtggcgca aaatacattc gcttcgagca
     1681 ctatcatcca ttctttccag gcggtttttc cggggtcatg gcgggcgcgg ccattgtctt
     1741 ttttacatac tttgggtttg attctgtttc tacggcggca gaagaatcca gaaatcctgc
     1801 acgcgacatg ccgatcggca tcattgcttc tctgatcgtc tgcgcgctgt tatacgtttc
     1861 cgtggccatt gttttgacgg ggatcgcgga ctatcgcact ctgaataatc cggcgcccgt
     1921 ggcaggagca ctcaaagcgc tgggtcttga ttccattcga agcctggttg gaacgggagc
     1981 cgttgtgggc attatttcct gcctgctcgt atttcaatat ggccaggccc gcatctggta
     2041 tgcaatgtcg cgtgacggtc ttcttcctgc ggcgttctcg cgcgttcatc caaaatttcg
     2101 cactccgcat atcagcacct ggattgcagg gctggtggtt ggaatcccgg cggggatctg
     2161 ggacattggc acgtttgcag atctggcaaa catagggaca ctggctgcgt ttgtgattgt
     2221 ttctgcgggc gtactgatac tacgacggac agaaccagat agaaagcgcg gctttcgagt
     2281 tccgtttgtt ccatggcttc ccattctatc catagtcacg tgtgcattcc tgatgatctc
     2341 tcttccgctg gagacctgga ttcgtttctt cgtatggatg gcgattggac ttgtgattta
     2401 tgtgctctac ggcaggcatc gcagttcgat ggcgcaataa aagtggcatc tggccggatt
     2461 ctacttgagc tttgcatgag aaaggtgcat tagacgcaat ggcccgcaaa gaaaacgcgt
     2521 ccgtcatcca gacagcctcc gcaaagcgac cgcctcttac aaaaaaactc atcctggatg
     2581 ccgctctcaa aatcattgag acagagggca tggagaagct cagcatgagg cgcatcgctg
     2641 cactcctgga ttgcagcgtg gccggaccct acgtatactt tacaaatcaa gaagaaatac
     2701 tcaaggcact catagacgac ggagaagcta ggctcacggc agaactcaag gctgccgtgc
     2761 agacaaaagt tgatattttt gataaaattt tcatactaat gcaaacatac aaatcgttcg
     2821 catccgcaaa ccgcgagcta cacaaactca tgtttgcaat gggtgggaga ggaaaaagct
     2881 tttctttgat ttcacccagt tatcgcgtct atttcgaaac ggtcagacag ggcgcaaaaa
     2941 atggagctat ccgttgcgat cgcgcaggct atcacgcaat cgcgcgcgcc atgtggtcct
     3001 ggggttacgg tctggtggtg ctcgagcttg cgcgtcatcc agaaactaag aaatccgaaa
     3061 ttgatccgat tgcagagggc atggaactgt tcaagaaact gctccaaccc tgagactaaa
     3121 cttccagttc ttcgggcgaa gaacgcctcc tctctttata tagtcgaatt tccttttcga
     3181 gcctttcata cgggtccatc cctgactccg gcagtcgaat ggacggagat ttcttcacaa
     3241 ctctggaaag gcc
//
LOCUS       DAORFF010000462         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_19200_length_3250_cov_3.521753, whole genome shotgun sequence.
ACCESSION   DAORFF010000462 DAORFF010000000
VERSION     DAORFF010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19200_length_3250_cov_3.521753"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PKK76_15705"
     CDS             <1..599
                     /locus_tag="PKK76_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69976.1"
                     /translation="LRSTRPLTASRIATDYFDRFRTGESNGVYSVAGATFGQDHLRHL
                     FDAVGSTTLGPAGLRQLDGNSAAYTAYVRDQFETDLLVHAALQEGVLDSPQASFFIEN
                     SMRHSMADYYLLTRIMRPDTNLRVVVSDQAVQSYYEKNREQYAKSGLPQKETMEIIRR
                     TLEQIQSDQMQAQIRLLRQQTINSLKDRGEHHFTGVSQ"
     gene            596..1270
                     /locus_tag="PKK76_15710"
     CDS             596..1270
                     /locus_tag="PKK76_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69977.1"
                     /translation="MIQSMSTFWFALRKPQATWASVSGAFLEILKGFLLIAGAASLLY
                     FVTTIPNLEKLRLAAMSLQARTGFASGGIMGSIGASLPWNRLLLLPVWTAGVFTASFV
                     RFMTLRFLGEKSATSGDIQLSKIASISMLGIAPLLVANALLACLGNFLPVSDIRQANP
                     VAFASVLAFVAGVFLEAHITIRIFRAEFSQTRGRAILTWMSPYLAGFCTCSVPTITML
                     FLMAVR"
     gene            complement(1314..3035)
                     /locus_tag="PKK76_15715"
     CDS             complement(1314..3035)
                     /locus_tag="PKK76_15715"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNL69978.1"
                     /translation="MRTRTIVAIAWTFLYSGPAWSQTVQGRVFDESGRPVAGAIVTVF
                     KEGSTIQESGYSDASGHFLVSSSFDGLRVRVRRPGYVDSIVPSASASSITLKAADAQA
                     LSDSLTASAHAAHVSWDSPEHKANFVSQCHYCHQIGNALTRAPKDQDTWKLTIDRMQG
                     YGALISYSDESSFRETLARSFDGKPIPFHQKHVVSPETFGAKITEWQMGEPDSFMHDA
                     EIHPNGKIYAVDMSKDKIYELDRKTNRIVAYDLPPSELPLGGMFSGAFRPLGTFAAHH
                     GPHSIQVGPDGKLWTTNSLAAEIMSFDPVTHAFKVYPVPDAIYPHTLRFDQKGRIWFT
                     VALSNKVGRFDPASGEFKILKLDSHGLFRLMSDLLLPTILKVSGWFPQKNLHVSLSHH
                     KATGEGHRILNLPYGIDVNPVDGSIWFSKLYGGFIGRVDPETLEVREWSSPLGGPRRL
                     RFAKDGTLWVPSFETSSLLRISTSTLKYKIYKLPLLNETEYETPYALGVHPVTQDVWI
                     TSNLSDRIFRFVPATEKFITYESPTRVTYMRDVLFDSDGTICTTNSNMPAYAIEGGRQ
                     KVFCLKP"
     gene            complement(3032..>3250)
                     /locus_tag="PKK76_15720"
     CDS             complement(3032..>3250)
                     /locus_tag="PKK76_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69979.1"
                     /translation="WLPIPRQTAVLALSADASTPLFFTEHDGSGLGEISAVIPGKQHL
                     YLANFRKNSRGVYSIPYPKGLQPEGEQK"
BASE COUNT          761 a          841 c          813 g          835 t
ORIGIN      
        1 gcctgagatc tacgcgacct ctgaccgctt cgcgcatagc aactgactac tttgatcgat
       61 ttcgcacagg agaatctaac ggcgtgtact cagtggccgg ggcaaccttt ggccaggatc
      121 atttgcgtca tctttttgat gctgtgggat ccaccactct gggtccggcc gggctcagac
      181 aattggacgg aaattctgcg gcctataccg cgtatgtgag agatcaattc gagacggacc
      241 tcttggttca tgcagcttta caggaggggg tactcgattc gcctcaggct tctttcttca
      301 ttgaaaattc aatgagacac tctatggcgg attactatct tctgacgcgc atcatgcgtc
      361 ccgatacaaa ccttcgtgtt gtggtaagtg atcaggcagt gcaatcctac tatgagaaga
      421 atcgcgagca gtacgcgaaa tcaggactcc ctcaaaagga gaccatggaa atcattcgcc
      481 gaactctaga gcagattcaa tcggaccaga tgcaagcaca gattcgcctg ctgcgacagc
      541 aaaccatcaa ctcactcaag gaccgcggcg aacatcactt cactggagtt tcacaatgat
      601 tcaatctatg agcacattct ggtttgccct tcgcaagcct caagcaacct gggcgagtgt
      661 ctcaggtgca ttccttgaaa ttctgaaagg attcttgctc atcgcaggag cggcttccct
      721 tctctatttt gtaaccacca ttcccaacct ggaaaaactg cggttggccg ccatgtctct
      781 ccaggcacgc actggatttg caagcggtgg aattatggga agcattggtg caagcctgcc
      841 ctggaataga ctgcttttac ttcctgtgtg gactgcaggc gtgtttacag cctccttcgt
      901 aagatttatg acccttcgct tcctcggaga aaagagtgca acgtcaggcg acattcagct
      961 atcaaaaatc gcatctatct ccatgctggg aattgcacct ctgctggttg caaacgcact
     1021 tctcgcatgc ctcggcaatt tcctgccggt ttcagacatt cgccaggcaa atcctgttgc
     1081 atttgcgtcc gttctggcat ttgtcgcagg agtttttctt gaagctcaca tcacaatccg
     1141 catctttcgc gctgagtttt cacagactcg cgggcgcgcg attctcacct ggatgagtcc
     1201 ttacctggca gggttctgta cctgttcggt tccaaccatt acgatgctat ttctgatggc
     1261 ggttcgctga accgccgcct cctacgcccg ccctttgcgc gctcgcattt tcctcaaggc
     1321 ttcaggcaaa acactttctg ccttcctcct tcaatcgcat aggcaggcat atttgaattc
     1381 gtcgtgcata tggttccgtc cgaatcaaac aacacatcgc gcatgtatgt gactctcgtt
     1441 gggctttcgt aagtgataaa cttttctgtc gcaggcacaa atcggaatat gcgatccgac
     1501 aggttcgatg taatccatac gtcctgtgtg actggatgca cgcccagagc gtatggagtt
     1561 tcgtattcag tttcgtttaa cagcggtaat ttgtagattt tgtatttgag agtgctggtg
     1621 ctgatgcgaa gaagagaact ggtttcaaag gaaggaaccc agagcgtacc gtcttttgca
     1681 aatctcaggc gacgcggacc acctagagga ctggaccatt cccgaacttc cagagtctct
     1741 ggatccactc ttcctatgaa accaccgtac agtttgctga accagatcga accatccact
     1801 ggattgacgt cgatcccata cggaagattg agaattctat gcccctctcc cgttgccttg
     1861 tggtgagaaa gagaaacgtg aagattcttc tgtggaaacc agcccgaaac tttcaggatt
     1921 gtgggaagaa gtagatcgct catcaggcga aacaatccgt gcgaatcgag tttgaggatt
     1981 ttgaactcgc cggaagctgg atcgaatcgc ccgactttgt tcgaaagtgc gactgtaaac
     2041 cagatcctgc ccttctgatc aaaacgcaga gtgtgcggat agatcgcatc gggcacagga
     2101 tacactttga atgcgtgagt gactggatca aatgacatga tttccgcagc cagagaattc
     2161 gtggtccaga gttttccatc cgggccaacc tgaatgctgt gaggtccgtg atgcgcggcg
     2221 aacgttccaa gcggcctgaa tgcgccggaa aacattcccc ccagaggaag ctcactcggc
     2281 ggcaggtcat acgcgacaat ccgattcgtt ttgcgatcga gctcatagat cttatctttg
     2341 ctcatgtcca ctgcatagat tttcccattg ggatgaatct ctgcatcgtg catgaaggaa
     2401 tccggttcac ccatctgcca ctctgtgatt ttcgcgccga atgtttcagg agacacaaca
     2461 tgcttctggt gaaacggaat tggttttcca tcaaaggatc gggccagggt ttctctgaaa
     2521 gaggattcat ctgaatagga aatgagcgca ccgtatccct gcatgcgatc gatcgtaagt
     2581 ttccaggtat cctggtcttt gggtgcacgg gtgagtgcgt ttccaatctg atgacagtag
     2641 tggcactggc ttacaaagtt ggctttgtgt tctggagaat cccagctcac gtgagctgcg
     2701 tgagcactcg cggttaaact gtcagacaat gcttgagcat ccgccgcttt cagagtgatc
     2761 gaagaggcag aagcagatgg aacgatcgaa tccacgtagc ccggtctgcg cactcgcacc
     2821 ctgaggccgt cgaagctgga agacaccagg aaatgaccgc tcgcatccga ataacctgat
     2881 tcttgaatgg tcgatccttc tttaaaaacg gtgacgatgg cgccggccac tggtctaccg
     2941 gactcatcga acactcttcc ttggactgtt tgagaccatg ccgggcccga atacaagaag
     3001 gtccaggcaa tggcgactat cgttcgtgtt ctcatttctg ttctccttct ggttgcaaac
     3061 cctttggata aggaatgctg tagacccctc gagaattctt tctgaaattt gccaggtaca
     3121 aatgctgttt gcccgggatg actgctgaaa tctcaccgag tccgcttcca tcgtgttcgg
     3181 tgaagaacaa aggtgtggac gcgtctgcag agagtgccag caccgcggtt tgtctgggaa
     3241 taggaagcca
//
LOCUS       DAORFF010000463         3244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_19251_length_3244_cov_4.258075, whole genome shotgun sequence.
ACCESSION   DAORFF010000463 DAORFF010000000
VERSION     DAORFF010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19251_length_3244_cov_4.258075"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1419)
                     /locus_tag="PKK76_15725"
     CDS             complement(178..1419)
                     /locus_tag="PKK76_15725"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010697.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNL69980.1"
                     /translation="MELNFQHADPGLSLMGEAAAARGLLESGKCKGSDFLGWLGLPSA
                     MVSQVSRIEEAAARIRKSDGLIVVGIGGSYLGARAVIEALGTDFPVVFAGHNMDSLYH
                     SDLLNSIQKKKYSINVISKSGTTTEPGLAFRILMNHMESRFSRDELAGLIFATTDANK
                     GSLRPLIKERGWTDFVIGDDVGGRFSVLSPVGLLPIAAAGKNIRRLLEGAQAMADIVR
                     KDSSEKNPALQYACYRNASYRSGKKIELFLSYQPGLHFIAEWWKQLYGESEGKEGKGI
                     FPASVDFSSDLHSMGQWIQEGERLIFETILDVIHPEKDIKIPSSPVDDGLEYLAGRSL
                     HEINRIALAAVQDAHSKGNVQSMKIMLDRMDEFNLGQLLYFYEYACAVSGIMLGVNPF
                     DQPGVEAYKKTMFRMLGKPGF"
     gene            1489..2256
                     /locus_tag="PKK76_15730"
     CDS             1489..2256
                     /locus_tag="PKK76_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69981.1"
                     /translation="MKRILIVLFFTISHSLYAFGTYSEGWAVIVPTKIESSGIIFDSY
                     EGLFDVVTFDRNEKCVEKEDLCYSPKSKSIAFSVRPENAVVANFVQKNVGREMLVQYR
                     IHRFTAIALSSELEITDAKAIQTQLPADVKKQVLVKKSGSKRSFSVYGKILQFEYRGR
                     FIGTYEGIYYDKQRDKVHQFSVTNEEIAKQLQLCMQTNVPFYFGVTVSYVTAWRESDY
                     DIFEINYDGPAGQAKVEPTDDSKKDAKTEEKAPEPAK"
     gene            2382..3092
                     /locus_tag="PKK76_15735"
     CDS             2382..3092
                     /locus_tag="PKK76_15735"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HNL69982.1"
                     /translation="MDFSIENKDGLTIVRFHVGNVSLFESRSLQSSIEKSTEDERVLA
                     LDLSSVEHMDSSALILFLKLSENLNLRGGFLILFGIRDSIKSLMTLTYLDHIVEIVAT
                     EADAIEIGTQTKNPREFVTSLNQALIFRGESRYHVIPYSEITHLESDGRATTVHTLKE
                     NYRTNKLLKDMETRIVSSIFLRIHKSYVVNMNYVNGMQYSVGGMYSIFLRDQANTSLP
                     VGRMYVQRIKALLGLGTD"
     gene            3174..3244
                     /locus_tag="PKK76_15740"
     tRNA            3174..3244
                     /locus_tag="PKK76_15740"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3206..3208,aa:Gly,seq:tcc)
BASE COUNT          866 a          838 c          755 g          785 t
ORIGIN      
        1 ggtcccgcag tgtttacaga atttggcggt ttccgcgttc tctttcgtac agtttgggca
       61 attcacagac tatcctaaaa gcctggcggc tcgacagagc ttgcggggcg agttttgcct
      121 gtcaaatggt tccgggaaga aagaggaaac ttttcgctcg cgaccggccc cgcagaacta
      181 aaaccccggc tttccgagca tgcgaaacat ggttttcttg taagcttcta cacctggctg
      241 gtcaaacggg tttaccccca gcatgattcc ggaaacagcg caggcatact cgtaaaaata
      301 caaaagctgg cccagattga actcatccat gcgatcgagc atgatcttca tggactgaac
      361 attccctttg ctgtgagcat cctgcaccgc ggccagagcg atgcggttga tttcgtgcag
      421 agaacgaccc gccagatatt caagaccgtc gtccacaggc gaagatggaa tcttgatgtc
      481 tttctccgga tgaatcacat ccagaatcgt ttcgaaaatc agacgctccc cttcctgaat
      541 ccattggccc atggaatgca gatcgcttga aaaatcaaca gacgcaggga agattccttt
      601 tccctctttt ccttctgatt ctccgtacag ctgcttccac cactctgcga tgaaatggag
      661 accgggctgg tagctcagaa acagctctat cttctttcct gaacgatagc tggcattgcg
      721 gtaacatgca tactggagtg ccggattttt ttctgaactg tccttgcgaa cgatatccgc
      781 cattgcctgg gctccttcga gaagcctgcg gatgttcttt ccggccgcag caatgggcag
      841 aaggcctaca ggagaaagca cactgaatct tccaccgaca tcgtcgccga ttacaaaatc
      901 agtccatccg cgttccttga taagaggacg cagacttcct ttgttcgcat ccgttgtggc
      961 aaaaatcaaa cccgcaagct cgtctctgga gaatctgctt tccatatgat tcatgagaat
     1021 gcgaaaagcg agacccggct cagtggtcgt gccgctcttt gaaattacgt tgatcgaata
     1081 cttttttttc tgaatcgaat tcagcaggtc ggagtgatag agtgagtcca tgttatggcc
     1141 ggcgaaaacg accggaaaat cagttcccag ggcttcgatg actgctctgg ctcccaggta
     1201 agatccgccg attccaacca caatgagtcc gtcgctcttg cgaatccggg ccgcagcctc
     1261 ttcgattctc gaaacctgtg aaaccatcgc ggacggaagt ccaagccagc ccaggaaatc
     1321 ggatcccttg cattttccgg attcaagcag accgcgtgct gcggcagcct cacccatcaa
     1381 agacagtcct ggatcagcat gctgaaaatt tagttccatt ttttactcct gagaaagcct
     1441 tgcagaaaca atcccactgt cttccaatgg gttcattcgt caacgcctat gaaacgcatt
     1501 ctaatcgttc tttttttcac catctcgcac agcctgtatg ccttcggcac ttactccgaa
     1561 ggatgggcgg tcatcgtgcc cacgaagata gaatcgtctg gaatcatttt tgattcctac
     1621 gaaggcctct tcgacgtggt tacctttgat agaaacgaaa aatgcgttga aaaagaagac
     1681 ctttgctatt cacctaaaag caaatccatc gcatttagcg taaggcctga gaacgccgtc
     1741 gtggccaact ttgttcaaaa gaacgtgggt cgcgaaatgc tggtgcaata tagaattcat
     1801 cgcttcactg ccattgcact ttcttcggaa ctggaaatca cagatgcgaa agccatccag
     1861 acgcagctcc cggcggacgt aaaaaagcag gtgctcgtaa agaaatcagg atccaaacga
     1921 agcttctccg tgtacggaaa gattcttcaa tttgaataca gaggccgttt catcgggacg
     1981 tatgaaggca tctactacga caagcaacgc gacaaagttc atcagttctc cgtgaccaat
     2041 gaagaaattg ctaaacaatt gcaactatgt atgcaaacaa acgttccgtt ttacttcgga
     2101 gtgacggttt cttatgtaac tgcctggaga gaatcggact atgatatctt cgagatcaac
     2161 tatgacggac cggcgggtca ggcaaaagta gaaccaaccg atgattcaaa aaaagacgca
     2221 aaaactgagg aaaaagcgcc cgaacctgca aaatgatgta atgaacgaca tgaaacaggt
     2281 aatgaacagc acataacggt gcaaccgctc taacttgttt taagtcttgc ctcccaaccc
     2341 gttcgtcata tctaagagcg atacacctgc gggttggcga tttggacttc tccatagaaa
     2401 ataaggacgg cttgacgatt gtgcgtttcc atgttggaaa cgtttccctg ttcgaatcca
     2461 gaagccttca gagttccatt gagaaatcaa cagaggacga aagagtactg gcactggacc
     2521 tgtcttctgt cgagcatatg gactcgagtg cactcatact gtttctgaaa ctatctgaaa
     2581 acctcaacct gcgtggcggg ttcttgattc tatttggaat cagagattcc atcaagtctc
     2641 tgatgaccct cacctatctg gatcacattg tggagatcgt ggccacagag gcagatgcca
     2701 tcgaaattgg gacccagaca aagaacccgc gagaatttgt aaccagtctc aatcaggctc
     2761 tgatcttccg gggtgaatca agatatcacg tcattccata ctccgaaatc actcacctgg
     2821 aatcggacgg gcgggccaca accgtgcaca cactgaaaga aaactataga accaataagc
     2881 ttctcaaaga catggaaacc agaatcgtct catcgatctt tcttagaatt cacaaaagct
     2941 atgtggtgaa tatgaactac gtcaatggga tgcaatactc tgtaggcgga atgtacagca
     3001 tcttcctgcg cgatcaggca aacacttccc ttccagtcgg aaggatgtat gtacagcgga
     3061 tcaaagcact ccttggcctg ggcacagact gattcctggc acgcctgtgg ccgccctgct
     3121 cgataaaaaa ccgattgacc ggggggcccc cgagccgcgt tttgtattca ccggcgggcg
     3181 tggtgtaatg gtagcacggc agccttccaa gcttctcgag agggttcaaa tcccttcgcc
     3241 cgct
//
LOCUS       DAORFF010000464         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_19274_length_3241_cov_3.932517, whole genome shotgun sequence.
ACCESSION   DAORFF010000464 DAORFF010000000
VERSION     DAORFF010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19274_length_3241_cov_3.932517"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKK76_15745"
     CDS             <1..299
                     /locus_tag="PKK76_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL69983.1"
                     /translation="VRFADRVRVKGKLEPVSLYEVVADKPRNSVRISQIPAFENAVSL
                     YQKGDLSAARKQFIELYKENTEDRAVRLYVRRIDEFLKNGLPENWDGVETLTSK"
     gene            complement(301..1047)
                     /locus_tag="PKK76_15750"
     CDS             complement(301..1047)
                     /locus_tag="PKK76_15750"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNL69984.1"
                     /translation="MDTGLKDSVVLVTGGTKGIGLAIAQAFQAEGAKVCICARSQEGL
                     DQCRERYALSGYQCDVTSAPSRKNLVESVLGDFGRIDVLVNNAGGSNGSTAMNTDLSL
                     YEEALQLNFLSLVDLSKQVAARMEKGVILNITSISGRESGGRATYNAAKAAAISFTKS
                     FADEMIRKGIRVVGIAPGSILHPTGNWQKRINENPEKMREFVEREIPAGRFGTVEEVA
                     SVAVFLASSKASWVVGATLNVDGGQSRMNF"
     gene            complement(1047..1952)
                     /locus_tag="PKK76_15755"
     CDS             complement(1047..1952)
                     /locus_tag="PKK76_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL69985.1"
                     /translation="MTGDTKDLEKEVKALRAEIAAFGESPYLKSSIKNLVYEAVIDRT
                     EVERNELGKRINEKHGTMYEIKEQARRMAILLGLDADNIRLLVTEAVQNILEHGSGRW
                     VQVRLDARNEGENPALVCAFKHEIPDSEKYTLQDAEANALKGDVTSEFFDFESPRGRG
                     EFIMKQLTDERRIINGVEINRDGKKVHYFKRILINYRNPEGVRDQLSFQEIKDQIDRL
                     GYQDVVCCFLIQHSFDKPDLFTVACSRDREKQVVQVMTHHSCSLVEQEAYFSNVFATF
                     APERPLTSAELQIVFDEVKSIVYGD"
     gene            complement(2020..>3241)
                     /locus_tag="PKK76_15760"
     CDS             complement(2020..>3241)
                     /locus_tag="PKK76_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69986.1"
                     /translation="NENVPRSAEFLSRRGALIKKTILSDPNRKGGVRYPGSLAVETLL
                     LVEPFSRQKYADQWKAEVASQHHSFKERLKKESNHWSDLILFLEEKDALSFPKEVWKR
                     ITEDKEILHHTWEKAGSTLHVFVRKDPEVFKVFVEGMTDLPVGEHWKILAMKSLLDRY
                     ETNFSQLFHDELFVRSYGKLLRIAYIDYIPWYYRIFIWLGLNWFQDKSFQIAKGAISI
                     EQKYLAGSNQQKSAELSQARETEKRQMMTRIQEMSAANQIVDALDRAYHTDLWIPSVA
                     EVQASIKDLDPSSFREALTRDRFQIVRDGKDSPDDILVYPMNHEWRVRAARLRRALDR
                     IMAKYPEGMQSDESRTMHERIRKLTRFLSRKEKEIPALDREDPYRKFEKALKTHEQKA
                     PKTASSPVAEDLEV"
BASE COUNT          783 a          860 c          732 g          866 t
ORIGIN      
        1 aagttcgctt cgcagatcgc gtgcgagtga aaggaaaatt agaacctgtt tctctctatg
       61 aagttgtggc ggacaaaccg cgcaattccg tcagaatttc gcagattcca gcctttgaaa
      121 atgctgtttc cctgtatcag aagggggatc tctctgcggc gcgaaagcaa ttcatcgaac
      181 tctacaaaga aaatacggaa gatcgcgcag tgcgactcta cgtcaggaga atcgacgaat
      241 tcttgaagaa tggactgccc gaaaactggg atggcgtaga aacactgacc agcaagtaat
      301 ttagaagttc attctggact gcccgccgtc cacattgagc gtcgcgccca caacccagga
      361 agccttgctc gacgcaagga atacggcaac actggccacc tcttcgactg ttccaaatct
      421 tcccgcagga atttcgcgtt ctacaaactc tcgcattttc tccgggtttt cgttgatcct
      481 tttttgccag ttcccggtag gatgaaggat ggagccaggc gcaatcccaa ccacccggat
      541 tccctttcgg atcatttcat ctgcaaacga ttttgtaaaa ctaatggcag ccgctttcgc
      601 ggcattgtat gtcgcgcgac cgcccgattc tctacctgaa atggatgtga tattcaagat
      661 cactcctttc tccatgcggg cagctacctg tttggataaa tccacgagcg aaagaaagtt
      721 cagctgcagc gcttcctcat aaagggaaag atccgtattc atagcagtcg atccattcga
      781 tccgcctgca ttgtttacca gtacgtctat gcgaccgaaa tcgcccagaa cggattccac
      841 aagattcttg cgcgaaggag cactggtcac atcgcactgg taaccggaaa gcgcataacg
      901 ttcccggcac tgatccaggc cttcctgcga cctggcgcag atacaaactt ttgcgccttc
      961 cgcctgaaac gcctgagcga tggccagtcc aatccccttt gtgcccccgg tcacaaggac
     1021 aacggaatcc ttaagcccgg tatccattag tcaccgtaga cgatcgattt tacttcatca
     1081 aagactatct gcagctccgc acttgtaagt ggtctttcgg gcgcaaatgt tgcgaataca
     1141 ttgctaaaat acgcttcctg ctccacaagc gaacaactgt ggtgggtcat tacctggacg
     1201 acttgcttct cacgatctct agagcaggcc actgtaaaca aatcaggctt atcaaacgaa
     1261 tgttgaatca aaaagcagca gaccacatcc tgatatccga gtctatcgat ctggtctttg
     1321 atctcttgaa aagaaagctg atcacgcact ccctcaggat tcctatagtt gatcagtatg
     1381 cgtttgaagt agtgcacctt ttttccatca cgattgatct caacgccgtt gatgatccgc
     1441 ctttcatctg ttagttgctt catgatgaat tcgccccgac cacgcgggga ttcgaagtca
     1501 aaaaactcgg aagtcacgtc tcctttgaga gcattcgctt ccgcatcctg gagtgtgtat
     1561 ttttcagaat caggaatctc atgcttaaaa gcacaaacga gggccgggtt ttcaccttca
     1621 tttcttgcgt ccaggcggac ctgaacccat cgaccggatc cgtgttcgag aatattttgc
     1681 actgcttccg ttacgaggag acgaatattg tccgcatcga gaccaagcag gatagccatt
     1741 ctcctggcct gctctttgat ctcatacatt gtgccgtgct tttcattgat gcgcttccca
     1801 agttcattgc gctctacttc tgtgcgatca atcacagctt cgtagactag attcttgatg
     1861 gaactcttca gatagggcga ttccccgaac gcagcaatct ctgcgcgaag cgctttgact
     1921 tctttctcga gatcttttgt gtctccggtc aaagcgttcc cccttccaga acatccggcg
     1981 aagcccgcca tttgtcaaag tatttttcca gcgcgagccc tagacctcca ggtcctcagc
     2041 tacaggcgaa gatgctgtct tcggagcctt ttgttcatgc gttttaagag ctttctcaaa
     2101 ttttctatac gggtcttctc ggtcgagagc ggggatttcc ttttctttgc gactcagaaa
     2161 tcgggtgagt ttacggatgc gctcgtgcat ggtgcgagac tcatccgact gcatgccttc
     2221 ggggtatttg gccatgatgc gatctaacgc tcgccgcagg cgggcagcgc gaacgcgcca
     2281 ttcatgattc attggataaa caagaatatc gtccggtgag tctttgccat cgcgcacaat
     2341 ctggaaacga tcacgcgtga gtgcctcacg aaaagaggat ggatcgagat ctttgatcga
     2401 agcttgcact tccgctaccg aaggtatcca caaatcagta tgatacgctc ggtcgagcgc
     2461 atccacgatt tgatttgcag cagacatttc ttgaatccgc gtcatcatct gtcgcttttc
     2521 tgtttccctg gcctgcgata gctctgcgga tttctgctgg ttcgaaccag ctagatattt
     2581 ctgctcaatc gatattgcgc ctttggcaat ttgaaaggat ttgtcctgaa accagttcaa
     2641 gcccaaccag ataaaaatgc gataatacca gggtatataa tcaatgtacg caatgcgcag
     2701 aagctttcca tagcttcgca caaacaactc atcgtgaaat agctggctga aatttgtctc
     2761 gtaccggtcc agcagagact tcattgcgag aatcttccag tgttcgccca caggcaggtc
     2821 tgtcattcct tctacaaaca ctttgaaaac ttctggatct ttgcgcacaa aaacgtgcag
     2881 agtactgcct gccttttccc aggtatgatg caatatttcc ttatcttccg tgattctctt
     2941 ccacacttcc tttggaaaag acagagcatc cttttcttcc aaaaacaaaa tcaaatcgct
     3001 ccagtgattg gattctttct tgaggcgctc tttgaatgaa tggtgctgcg aagccacttc
     3061 cgctttccac tggtccgcat acttctggcg agagaatggc tctactagaa gaagcgtttc
     3121 gactgcgaga gatccggggt atcgcacacc accctttcga tttggatcac tcagaattgt
     3181 cttcttgatc aaggcgccac gtctggagag aaactcggcg gagcgcggga cgttttcatt
     3241 t
//
LOCUS       DAORFF010000465         3235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_19334_length_3235_cov_4.398113, whole genome shotgun sequence.
ACCESSION   DAORFF010000465 DAORFF010000000
VERSION     DAORFF010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19334_length_3235_cov_4.398113"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PKK76_15765"
     CDS             <1..459
                     /locus_tag="PKK76_15765"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNL69987.1"
                     /translation="FAAIHFWYPKFMGRMPNETLGKWHFWITAIAFNGIFLPLFFTGL
                     MGEHRRIFDYSAWPSLMTDEMKLYRKFATTSLLVLLAAQPLFFINMIYSYFKGAWAVN
                     NPYKANSLEWSVPSPPPHGNFTEVPTVYRGAYEFSVPGREADYWPQDAKS"
     gene            489..1064
                     /locus_tag="PKK76_15770"
     CDS             489..1064
                     /locus_tag="PKK76_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNL69988.1"
                     /translation="MSSVATNQKVEFGRVPLGRIAMWWVIASEVVIFGGLITCYLLYR
                     IRYPEWGHEAAHTNTWLGALNTFVLLTSSFTIVLAHAASTKGELKKVWLYSLCTILGG
                     FIFLGVKAVEYTTEIHHGFTITKSVFWSFYYVMTGLHASHVIAGMTAIFVVALKARKG
                     EHLHRVEMAGLYWHFVDVVWIFLFPLLYIAK"
     gene            1080..1397
                     /locus_tag="PKK76_15775"
     CDS             1080..1397
                     /locus_tag="PKK76_15775"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNL69989.1"
                     /translation="MSDHQQQNGHEDYHGHPNYFVIWAVLVVILGISLGLGYLGNHTL
                     AVTLIFALAFVKAFLVLANFMHLRWEPKAVTAIIIFSMIVLGYVFLGVYPDIVPIERI
                     IAQ"
     gene            1398..1919
                     /locus_tag="PKK76_15780"
     CDS             1398..1919
                     /locus_tag="PKK76_15780"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HNL69990.1"
                     /translation="MDSTGRQNNVFFGMMLLLTTEAMFFSGLISAYLVARGNAAVWPP
                     ADQPRLPMFITAINTMILVLSGLTAVVSFRKKSVRLMILAAVLGLLFLLIQGSEWVRM
                     LSFGLTTVSSIYGAFFYLIVGAHGIHVAAGLLLLLLLLWKTKSGSVWIAGTIYWAFVV
                     AIWPVLYFLVYIL"
     gene            1916..2122
                     /locus_tag="PKK76_15785"
     CDS             1916..2122
                     /locus_tag="PKK76_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69991.1"
                     /translation="MKTIFALISFALAVVPAELFACGACIGRGYSQSLIDSYVVITIL
                     LSGLPLIFGGLLIYYMVKRSKSGQ"
     gene            2122..2556
                     /locus_tag="PKK76_15790"
     CDS             2122..2556
                     /locus_tag="PKK76_15790"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNL69992.1"
                     /translation="MDLSSLPPINATLNGLSFLLQVSGYIAIRTGRRSLHRALMLSAV
                     TVSAIFLTSYLYLHAHVGSIPFGGQGFARVAYFSILISHSILAACTLPLVITTVTLAL
                     RGSVKHPKWGRVTILVWGYVSVTGVIVFLMMLPYYPRAFSPG"
     gene            2577..3200
                     /locus_tag="PKK76_15795"
     CDS             2577..3200
                     /locus_tag="PKK76_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69993.1"
                     /translation="MRQRIWTCAACFLFLLSLCLPGSADAQEFFHYRIFTPESGDLRE
                     LPDSSSESLATLSRGVALMETGMRDRQIVVQGKDGDWIQVAYFGDVGWVPLNHTALPA
                     SIRTVSTSAEFLGTWRSNLKCRIQSYIHIEADGMFYGSHCISCTMEGCPESISGKWTI
                     EDGMLVLTERKGQKAQYFIKDGVLVSDPTNGFIWEYHGASVLSGLRK"
BASE COUNT          690 a          822 c          796 g          927 t
ORIGIN      
        1 tttgccgcca ttcacttctg gtatccaaag ttcatgggaa ggatgcccaa cgaaactctg
       61 ggtaaatggc atttctggat cacagcaatt gcgtttaacg gtatcttcct cccgctcttc
      121 ttcacaggcc tgatgggaga acaccgtcgt atttttgact attctgcatg gccaagtctc
      181 atgacagatg agatgaagct ttatagaaag tttgccacca cctctcttct cgtgttgctc
      241 gcggcgcaac cactgttctt catcaacatg atctacagct acttcaaagg tgcatgggca
      301 gtaaacaatc catacaaggc aaattcgctt gagtggtctg ttccttcccc tccgccgcac
      361 gggaacttca ccgaagtgcc taccgtttat cgcggtgcat atgaattcag tgtgccaggt
      421 cgcgaagctg actactggcc acaagatgcg aagagctaaa aggcaaacca aactggagat
      481 atgataaaat gagttctgtt gccactaacc aaaaagttga atttggccgc gtccctctgg
      541 gacgcattgc aatgtggtgg gtgattgcat ccgaagttgt aatcttcggt gggctcatca
      601 cctgctacct tctctatcga atccgctatc cggagtgggg tcacgaagcg gctcacacaa
      661 acacctggct tggggcgctg aacactttcg ttctgcttac gtccagtttc acgatcgttc
      721 tggctcacgc ggcttccaca aaaggagagc tgaagaaagt gtggctctat tctctctgca
      781 caattctcgg gggtttcatc ttcctgggcg ttaaggcggt agaatacacg actgaaatcc
      841 atcacggctt cacaatcacc aagagtgttt tctggagctt ttactacgtg atgactggcc
      901 ttcacgcgtc gcacgtaatc gcaggaatga cagccatctt tgtggtcgca ctcaaagcac
      961 gcaaagggga acatctgcat cgcgtcgaaa tggcaggcct gtactggcac tttgttgacg
     1021 tggtctggat tttcctgttt ccacttcttt acattgcaaa ataatttctg gagaagcaaa
     1081 tgtcggatca tcagcagcaa aacggacacg aagattatca cggtcatccc aactattttg
     1141 tgatctgggc cgttctggta gtcatcctgg ggatcagtct tgggcttgga taccttggca
     1201 atcacactct ggcagtgacg ctgatcttcg ctctggcttt cgtgaaggca ttcctggtgc
     1261 tcgcgaattt catgcatctt cggtgggaac caaaggcagt cactgcgatc atcatcttct
     1321 caatgatcgt tctaggatat gtctttctcg gtgtctatcc agacatcgtt cccattgaaa
     1381 ggatcatagc acagtaaatg gattcgaccg gaagacagaa caacgtcttt tttggaatga
     1441 tgttgcttct aaccacagaa gcaatgttct tttccggtct catcagcgcc tatctcgttg
     1501 cacgcggcaa cgcggccgtt tggcctccgg cagaccagcc caggttgccc atgttcatca
     1561 ctgcgatcaa tacaatgatt ctggtgctca gtggtctcac ggctgtagtt agctttcgca
     1621 agaaatcggt tcgccttatg atcctcgccg ccgtactcgg gcttctgttc ctcttgattc
     1681 agggatcgga atgggtgcga atgctttcct ttggtctgac cacagtatca agtatctacg
     1741 gagcgttctt ctatttgatc gtcggggcac atgggattca cgtggcagcc ggcctgctcc
     1801 ttttgcttct gcttctctgg aagactaaga gtggatctgt gtggattgcg ggaacgattt
     1861 actgggcctt tgtcgtcgcc atttggccag ttttgtattt tctggtgtac atactatgaa
     1921 gacgattttc gcgctcatct catttgcact ggcagtggtt ccggcagagt tgtttgcctg
     1981 cggcgcttgc atcggtcgcg ggtacagtca atcgctgatt gattcttatg tagtcattac
     2041 gatcttgctc agcggtcttc cacttatctt tggcggcctt ctgatctact acatggtcaa
     2101 acgatccaag tcaggacagt gatggatctc agttctcttc cccccatcaa tgcaactctc
     2161 aatggccttt cctttctact tcaggtgagc gggtacattg cgattcgcac aggtcgtcgt
     2221 agtctgcatc gcgcgctgat gctttctgcc gttaccgtat ccgcgatctt cttgaccagt
     2281 tatctgtatt tgcatgcgca tgtgggcagc atcccgtttg gcggccaggg atttgcacga
     2341 gtcgcctact ttagcatttt gatcagtcat tcaattctcg cagcatgcac tcttccgctt
     2401 gtgatcacaa cggttaccct cgcgcttcgc ggatccgtca aacatccaaa gtggggccgc
     2461 gttacaattc tagtctgggg gtatgtgtcg gttacggggg tcatcgtgtt cttgatgatg
     2521 cttccctact atcctcgcgc cttttctcct ggatgattct ggatttgcat gtctatttgc
     2581 gtcagcgcat ctggacgtgc gctgcatgct tcttgttttt gctctcgctt tgtctgcctg
     2641 gatctgcgga cgcacaagaa ttctttcatt acagaatctt cacccctgaa tccggcgatc
     2701 tgcgcgaact tcccgattct tcttccgagt ccctggcaac tctcagtcgt ggtgtggccc
     2761 tcatggaaac cggaatgcgc gacaggcaaa tcgttgttca gggaaaggac ggagactgga
     2821 ttcaggtagc atacttcgga gacgtgggct gggttcctct caatcatacc gcactgccag
     2881 cgagcattcg cacagtctct acttccgcgg agtttcttgg cacctggcgt agcaacttga
     2941 agtgcaggat tcagagttat attcatatag aagccgatgg aatgttttat ggttcgcact
     3001 gcatttcctg caccatggaa ggttgtccgg aaagcatttc gggaaaatgg acgatagaag
     3061 acggaatgct tgttctcacc gagagaaaag gccagaaagc acagtacttc atcaaagacg
     3121 gtgttcttgt atctgatcca accaatggat tcatctggga atatcacggc gccagtgtcc
     3181 tttcagggtt gcgcaaatga gagaagtacg tgtcgtcgcg tttctttttc tcatc
//
LOCUS       DAORFF010000466         3234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_19341_length_3234_cov_3.843032, whole genome shotgun sequence.
ACCESSION   DAORFF010000466 DAORFF010000000
VERSION     DAORFF010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19341_length_3234_cov_3.843032"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PKK76_15800"
     CDS             <1..1035
                     /locus_tag="PKK76_15800"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNL69994.1"
                     /translation="DVCLLGGIAMLYQMTGSFDFDEIFARKNELASTHGSNFHFAGVL
                     FAAGAMTKSAQFPFHFWLPETMETPTTVSALMHAGIINAGGFLIIRMSPLLHLATWAL
                     TLLTAVGAITAVFGALVMATQNDIKKKLAYSTISQMGMMMFGCGLGAFSIALFHIFAH
                     SFYKAHAFLSTGNLTAESKKRGLKLQAPSMFILLLTSMIGLAIIAIGVNYHSGDYLAY
                     FVYGAVLQLGLFQNIGHADLSSSSRIRVTAITAIGLVLAVTAYAILEHAIGFTVLGIV
                     GPATDGTSSAFIPIAAYLLFVASLFLCAQLMRPGQSDWEKRLYVYFWNGGYFSHWSTR
                     WLASPDLQNR"
     gene            1077..>3234
                     /locus_tag="PKK76_15805"
     CDS             1077..>3234
                     /locus_tag="PKK76_15805"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HNL69995.1"
                     /translation="MITQERKPEIEKSIETSLMIAAPSWPLQNTVAVNPFWFLKDKLF
                     RDVVVNLAPVVNSPLLMPMEFYLQRYEEGTVTAKALEDAMRDARPHSPDLPHSLDSFL
                     MQSKDAHTRLRALPTFAEFLDTDGYWQRTVTNDLGKFAAGYFDDDQAIFPFPWKSLSF
                     WQAWCEARAYDQAMEICGASGFNASVAPLKSMSCSEAVIYMIREMDFKTDEARTLYMQ
                     RLIASILGWATRFKYVEWQTMLGYDQIRSSTAMELLCVRLAYDFGLFHTTRKSSSPEW
                     REEYEKIIETDFASQLTLVWQAALERSYQDRVAGVLQRPSPQDHSLWQMAFCIDVRSE
                     TIRRHIEEQDSRVKTIGFAGFFGVPLAYRKLEERDTSLRMPVLLSPAFTVKEVPRSPD
                     RYRTEDGPTDVFVLSYFRNLRKSALSSFLFVELFGILSIENIVRKTLVSIIRRLKGKA
                     IPARFDDRKYRPAQETLSPDGNPEGMEVRVQRAITILKRMGLREPFGRLVFIVGHGSV
                     TNNNAFGSALDCGACGGHAGDINARYLADLLNDSEVRVRLAQLGIQIAQTTWFVAAVH
                     ETVTDEVFFLDEETIPVAFKMDLEMVRGILNSASSRARAERQKAFKNLSVSSAQTRGQ
                     NWSEVRPEWGLAGNACFIVAPRERTRGVNLGSRAFLHDYNYAIDDEFKTLELIMTAPM
                     VVTNWINMQYYASTVANGVYGAGSKVLHNLVAEMGVL"
BASE COUNT          763 a          839 c          819 g          813 t
ORIGIN      
        1 gacgtttgtc tcctgggcgg aattgcgatg ctgtatcaaa tgactggctc tttcgatttt
       61 gacgaaattt tcgcgcgcaa aaatgaactc gcttcaacgc acgggtctaa cttccatttt
      121 gcgggagtgc tttttgcggc aggggccatg accaaatcgg cgcaatttcc atttcacttc
      181 tggctcccgg agacaatgga aacgccaaca actgtctcgg cactcatgca cgccggtatc
      241 atcaatgcag gagggtttct catcattcgg atgagcccac ttcttcacct ggcaacctgg
      301 gctcttacat tgctcacggc tgtcggagcc atcacggcgg tattcggcgc cctcgtgatg
      361 gccacacaaa atgatatcaa gaagaagctt gcttactcaa cgatcagcca gatggggatg
      421 atgatgttcg gctgcgggct tggtgcattc agcatcgctt tgttccatat cttcgctcat
      481 agcttctaca aagcgcacgc atttctttcc acgggcaatt tgaccgcaga atcaaagaaa
      541 cgtggtttga aattacaggc tccgtctatg ttcatactcc tcctcacgag tatgattggc
      601 cttgcaatca tagcaatcgg ggtgaactat cattccggtg attacctggc gtattttgtc
      661 tacggcgctg tcttgcaact cgggcttttt cagaatattg gacacgcaga tttaagttct
      721 tcttcgcgta ttcgggtgac ggccatcaca gcaatcggtc ttgtactggc agtcacagcc
      781 tacgccatcc tcgaacacgc catcggcttc actgtgttag ggatcgttgg cccggctact
      841 gatgggacat cgtcggcgtt cattccgatc gccgcctatc ttctctttgt cgcgagtttg
      901 ttcttatgcg ctcaactcat gcgaccggga caaagtgact gggagaaaag actctacgta
      961 tacttctgga atggcggata tttttcacac tggtccacgc gatggctcgc ttcgcctgac
     1021 ttgcagaaca gataagttca ttcctgatcc aagagaattc gaaccaggag aagagtatga
     1081 tcacgcaaga aagaaaacca gaaatcgaaa agtccattga aacgagcctg atgatcgcgg
     1141 ccccctcatg gcccttgcaa aatacagtgg cggtcaatcc gttctggttt ctcaaggaca
     1201 agctcttccg cgacgttgta gtgaatcttg cacctgttgt aaattcgccg ctcctgatgc
     1261 ccatggaatt ttacctgcag cgttatgaag agggtactgt cactgcaaaa gcgctcgaag
     1321 atgcaatgcg agatgcgcga ccacattcac ccgacttacc acattcgctg gattcttttc
     1381 tcatgcagtc gaaagacgcg cacacgcgac tcagagcgtt gccgactttc gcggaatttt
     1441 tagacacgga tggatactgg caacgcacag ttacaaacga tcttggaaaa ttcgccgcag
     1501 gctactttga cgacgatcag gcaatctttc cctttccctg gaaatcttta tctttctggc
     1561 aggcctggtg cgaggcgcga gcttatgatc aagctatgga gatttgcggt gcttcaggct
     1621 tcaacgcatc cgtggctcca ttgaaatcca tgagttgctc cgaggctgta atctacatga
     1681 tccgcgaaat ggatttcaag actgatgaag cgcgcactct ttacatgcaa aggctcattg
     1741 cgagcatcct tggatgggcg acccgtttca aatacgtcga gtggcagacg atgctcgggt
     1801 atgatcagat tcgatcttcc actgcaatgg aactactttg cgttcgcctc gcatatgact
     1861 ttggtctatt ccatacgact cgcaagagtt cctcccctga gtggagagag gagtatgaga
     1921 aaatcataga gaccgacttt gcctctcagc tcacgctcgt ttggcaggcg gcccttgagc
     1981 gaagttacca ggaccgcgtg gccggagtac tccagcgtcc ttcgccgcaa gaccattcgc
     2041 tctggcaaat ggcattctgc attgacgtgc gctccgaaac cattcgccga cacattgaag
     2101 aacaagactc gcgcgtaaaa acgattggtt ttgccgggtt cttcggggtt ccccttgctt
     2161 acagaaagtt ggaagaaaga gacacgagcc tgcggatgcc tgtactactg agtcctgctt
     2221 tcacggtcaa agaggttccc aggtcgccgg atcgctaccg cacggaagac ggtccgactg
     2281 acgtatttgt attgagctac tttcggaatt tgcgcaagag cgctctctct tcatttcttt
     2341 ttgtcgagct cttcggcatt ctttccatag aaaacatcgt gcgaaaaacg ctggtctcaa
     2401 tcatcagaag attgaaagga aaagccatcc ccgcaagatt cgatgatcgg aaatatcgcc
     2461 cggcccagga aacgctttcg cctgacggaa atccagaagg catggaagta cgcgttcaac
     2521 gcgccatcac gattctcaag cgtatgggtc tgcgcgaacc tttcggccgc cttgtgttca
     2581 ttgttgggca tggaagcgtt accaataaca atgcgtttgg atctgcactc gactgtggag
     2641 catgcggcgg acatgcaggc gatatcaatg cacggtacct cgctgacttg ctaaacgatt
     2701 cggaagtccg tgtgagactc gcgcagctcg gcatccaaat tgcgcaaaca acctggtttg
     2761 tcgccgcagt ccacgaaact gttaccgacg aagttttctt tctagacgaa gaaacaattc
     2821 ccgttgcgtt taagatggat ctggaaatgg tgcggggtat tctcaacagt gcgtcgtcgc
     2881 gtgctcgtgc tgagcgccag aaagcgttta agaatttgag cgtctcaagc gctcagacgc
     2941 gtggacagaa ctggtctgaa gttcgcccgg aatggggcct ggccggtaac gcatgtttca
     3001 ttgtcgcgcc acgagagcgg acgcgcggag taaatctggg cagtagagct tttcttcacg
     3061 actacaatta tgcaatcgat gacgagttca agacactgga actaatcatg actgctccca
     3121 tggtcgtcac gaactggatc aacatgcagt actacgcctc gaccgtggca aacggtgtct
     3181 acggtgcagg cagcaaagta ctccacaatc tcgtggccga aatgggtgtt cttg
//
LOCUS       DAORFF010000467         3228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_19407_length_3228_cov_3.628427, whole genome shotgun sequence.
ACCESSION   DAORFF010000467 DAORFF010000000
VERSION     DAORFF010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19407_length_3228_cov_3.628427"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1557)
                     /locus_tag="PKK76_15810"
     CDS             complement(<1..1557)
                     /locus_tag="PKK76_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69996.1"
                     /translation="MLTWDANLRMLQTLDVRDDFVHGKSFRAIGNILESPTWPPLHGL
                     IALVAYLFTSPSTVAEAGIGMLFFALTIALLAAMAAYIGKSEDMMISASLSFVASVII
                     LSHTRELAAYATSAMLETQGMFFLALTMLALYRSFRNGKGITLLTFSALGLFLTKYPY
                     GHILVMAVVPVAIAAHFDSIGPMISFLRKRYRGVALILPGALMLLIAALAMARFLPEG
                     ILKVKGFKYVFYGLVVLLWVDLNWKFYQARAELNWIPPVWKTIYISVVTPILFLTFLN
                     PDRFSSTVGTQLHEQDSARSFLLSFFDLTDSPVPLFAFAGLLVVSIVFAAIKRKSKLT
                     GDPLFAMLLVLIMHVLILEFLTANKQLRHLYHLLPAILLSGMVFALREIRYPIRIIAG
                     FILLGSASILIASKGSVLKAEHYMFRYHCWTGVEANMYEPVRTLVSQLPAQKRYMLIN
                     RFHEPDAPLPGRLYATDFDLLIRMKPGTSARNDSRYQWKSWSEFDSVLLLQRECNDPV
                     GENLLFTRAQT"
     gene            complement(1668..2228)
                     /locus_tag="PKK76_15815"
     CDS             complement(1668..2228)
                     /locus_tag="PKK76_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69997.1"
                     /translation="MIYKKLILIVSLLALFSTECRTVRPRDMIPGLERGRVIYSEGAR
                     KPTEAELTAVDLLLKQLLSMVQRKDWSDLPGLVSKKKGIYVDLKGFRSFAQLEADVRD
                     RGSYLYVFYHDTDTLRAFTHDKDQIAVREVLMSGNSIRVDYYMEEGSQECELALHATG
                     NEKEDYRLNHPVFIREDGVWKVYRLF"
     gene            complement(2225..>3228)
                     /locus_tag="PKK76_15820"
     CDS             complement(2225..>3228)
                     /locus_tag="PKK76_15820"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNL69998.1"
                     /translation="EFETAAALAPNDPTPTFFLAFQHYRQREFDRAREAIERTLPLVM
                     DPIVLHLANRLATVIYFHVKDYPRAISCANQCLATAIESDLKREEYDARLSLAILGIA
                     SSNLELANDQLLELELVNPSDLTVQKLADFRMDLEEGSAALDRISPRGFDFNAQIQDW
                     LRNRFPEDMIYRLSGLEMEDEFNIDDYFTSEGEVQKKKVESTIDPALMIERFNTLTDV
                     PFQNACAKIISLQGFQLEKTLPYREKDGGDFVAANKNDKKVKALFRFRKWKNQPISDI
                     YLREMQNLMNEMKMNQGFFVAGTRLTPGAESALANLKKITVLHDIELAEILFKVLGQ"
BASE COUNT          834 a          793 c          810 g          791 t
ORIGIN      
        1 tgtctgagcc cgcgtaaata gcaagttttc accgactgga tcattgcatt ctctttgcag
       61 caagagcacg ctgtcaaatt cgctccagga tttccactgg tagcgtgaat cgtttcgagc
      121 gcttgtgccg ggtttcatcc gaattaagag atcgaagtcg gtcgcgtaga gccttcccgg
      181 aagaggtgcg tccggttcat gaaagcgatt gatgagcatg tatctctttt gtgctggaag
      241 ttggcttaca agtgtgcgaa caggttcata catgttcgct tccacgccgg tccagcaatg
      301 atagcgaaac atataatgct ctgcctttaa gacacttcct tttgaagcta tcaaaatgga
      361 tgcgctccca agcaaaatga atccagcgat gatgcggatc ggataccgga tttccctgag
      421 tgcaaagacc atcccactaa gaaggatcgc ggggagtaga tggtataagt gacgcagctg
      481 cttgttcgca gttagaaatt cgaggatcaa cacgtgcatg atgagcacga ggagcattgc
      541 gaacaatgga tctccagtaa gtttggactt tcttttgatc gcggcaaata cgatcgacac
      601 aacaagcaag ccggcaaaag cgaataaagg caccggagaa tctgtaagat cgaagaaact
      661 cagtaaaaag cttcgtgcgg agtcctgttc gtgcaactgt gtccctacgg tgctcgaaaa
      721 ccggtcgggg ttcaggaatg tcagaaagag aatgggtgta acaacagaga tatagatcgt
      781 cttccagacg gggggaatcc agttcaactc cgcgcgcgcc tgatagaatt tccaattgag
      841 gtcgacccag agcaagacca cgagtccgta aaagacatac ttgaagcctt tcacttttag
      901 aattccttcc ggaagaaaac gcgccatggc gagagctgcg atcagaagca taagagcgcc
      961 gggcagaatc agggccacac cccgatagcg ttttctgaga aaggagatca tcgggccaat
     1021 cgaatcaaaa tgcgcagcga ttgctacagg aacgactgcc attacaagga tatggccata
     1081 aggatacttc gtgagaaaga ggccaagggc actgaacgtc agcagtgtga ttcctttgcc
     1141 gttgcgaaaa ctcctgtaaa gtgcgagcat cgtcagtgct agaaagaaca tgccctgagt
     1201 ctcgagcatg gcggaagtag cataggcggc gagttcccgc gtatgactga gaatgatcac
     1261 actcgccaca aagctcaatg aagcggagat catcatgtcc tctgactttc ctatgtacgc
     1321 agccatagct gcgagcaagg cgatggtaag agcaaagaat aacatcccga tgcctgcttc
     1381 cgccactgta gacggagacg tgaacaagta agcgaccagg gcgatcaagc cgtgaagggg
     1441 aggccaggta ggggattcca gaatgttgcc aatggctcga aatgattttc cgtgcacaaa
     1501 gtcatcgcga acatccagag tctggagcat tctcagattg gcgtcccacg tgagcacatc
     1561 cctgttcggg caagctgcaa attctgtcca atccctttga atcttccatc ctccaactaa
     1621 cagcccaaag gcgatcaagc ccagagagaa cggaatcgat gcctttttca gaatagacga
     1681 tagactttcc aaactccgtc ctcccgaatg aacacaggat gattcaagcg gtagtcttct
     1741 ttctcatttc cggtagcgtg cagagcaagc tcgcattcct gactgccttc ttccatatag
     1801 taatccacgc gaatagagtt tcctgacatg agcacctctc gcacggcaat ctggtctttg
     1861 tcatgagtga aggctcgcag tgtgtctgta tcatgataga acacgtacag gtagctaccg
     1921 cgatctcgga cgtcggcttc caattgcgca aacgatctaa agccttttag atccacgtat
     1981 atacccttct tcttgctgac cagtccaggc agatcgctcc agtcctttct ctgcaccatg
     2041 gacagaagtt gtttgagtag caaatctacc gctgtaagtt ccgcttccgt tggctttcgc
     2101 gcaccctctg aatagataac gcgtcccctc tcgagcccgg ggatcatgtc gcgcgggcga
     2161 accgttcggc actcagtcga gaatagagca agaagcgaga ctatgagtat gagctttttg
     2221 taaatcactg gcccaggacc ttgaagagga tctctgccag ttcaatgtcg tggagaacag
     2281 taattttttt cagattcgcg agcgcggatt ctgcacctgg agtcaggcgc gttcctgcca
     2341 caaagaagcc ttgattcatt ttcatctcgt tcatcaggtt ctgcatttct ctcaaataga
     2401 tatcagagat gggctggttc ttccactttc taaagcgaaa gagcgctttc acctttttgt
     2461 cattcttgtt tgccgcgaca aagtctccgc catccttttc gcggtaaggc aaagtcttct
     2521 cgagctgaaa tccctggaga gaaatgatct tagcgcatgc attttgaaag ggaacgtccg
     2581 ttagagtgtt gaatcgctcg atcatcaacg ccggatctat ggtcgattcc accttcttct
     2641 tttggacttc cccttcggaa gtgaaatagt cgtctatgtt gaattcgtct tccatctcga
     2701 ggccggacag tcgatagatc atatcttccg ggaatcgatt gcgaagccag tcctgaattt
     2761 gtgcattaaa atcaaagccg cggggactga ttcgatcgag cgcggcactg ccctcttcta
     2821 agtccatgcg aaagtcagcc agcttttgca ctgtcaggtc ggacgggttt acaagctcaa
     2881 gttcaagcaa ctgatcattg gcgagttcta gattgctcga agcgatcccg agaatggcaa
     2941 gactcaagcg tgcgtcatac tcttcccttt ttagatctga ctctatggct gtggccagac
     3001 actggttcgc gcacgatatg gctcgcggat aatctttcac atgaaagtag attacggtgg
     3061 ccaaacgatt cgccaggtgc aaaacaattg ggtccatgac cagaggtaga gttctctcaa
     3121 tcgcctcccg agctctatcg aattcccttt gtctatagtg ttgaaatgct agaaagaatg
     3181 ttggggtggg atcatttggt gccagagctg cggcagtttc gaattcgc
//
LOCUS       DAORFF010000468         3222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_19461_length_3222_cov_3.542785, whole genome shotgun sequence.
ACCESSION   DAORFF010000468 DAORFF010000000
VERSION     DAORFF010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19461_length_3222_cov_3.542785"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..627
                     /locus_tag="PKK76_15825"
     CDS             115..627
                     /locus_tag="PKK76_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL69999.1"
                     /translation="MKFWEKEKEDNTKELILLLAAAYLANPCNFTASPVLTSAQPSGT
                     MTGVAGSIKGKLLTRAGTPAISALVIAEDNAAIGVNYWATHSSINRDGSFQISGIPGG
                     TTVKLSFESILSDYNGRIDGHIDCFKTPSTFTAGWVTGGTLTTTRASGQNFSIVNATT
                     LDAGTIYLVN"
     gene            627..1445
                     /locus_tag="PKK76_15830"
     CDS             627..1445
                     /locus_tag="PKK76_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HNL70000.1"
                     /translation="MPELPDLVYVARGLHDFISGRIIEQVEVREPIVVRNLTGEPFGA
                     CVTGRGILNVRRHGPFLVFELTGHRMIVAHPMLAGAFSIEPKSAGLITFIFKEGKLSY
                     LDAKKMGKIYVVDPGQEAQIPRFTTQGVDITSPEFTEDVFLGLIRTSRRQVRAFLMEQ
                     EQISAIGNAYADEILFEARIHPKTFCHQLGPDEQKVLYDSIRTVIDRGIKEVQGANAG
                     LHVKHRDHMLVRNKKNEPCPRCGAKIRRESVLGYDTFFCPVCQPAKRKLFIDWK"
     gene            complement(1429..2565)
                     /gene="hisC"
                     /locus_tag="PKK76_15835"
     CDS             complement(1429..2565)
                     /gene="hisC"
                     /locus_tag="PKK76_15835"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNL70001.1"
                     /translation="MKPSEFVHSHIRHLDGYVPGLQTEDPEVIKLNTNENPFPIPASV
                     RNALVQEIDGKLLQKYPNPVSQRLREALAAKYGRPPECFLVGNGSDEILSILFRAVLG
                     QDKAMIAPYPTYSLYPVIAALSHSSCIEIQVREDWTLDFEAMLQKIQKGGIALAAFAN
                     PNAPTGIAESEKDILDFTSRNPSLTLVDEAYADFGNVTVFKNAGTSTYPRLLVSGTAS
                     KALSLAGARIGWLCAEESLIAELEKIKDSYNVSRLAQAAALAAVLDKSEFEKRRDEIV
                     NNRNFLTEGLDRLGFETLPSSTNFIFTRPPADLRALSQRGETAAGAYYRLLLENKILV
                     RYWSSEKLKEWIRITIGTRADMEKLLSTTDSIVSLAKREPISSQ"
     gene            complement(2562..>3222)
                     /locus_tag="PKK76_15840"
     CDS             complement(2562..>3222)
                     /locus_tag="PKK76_15840"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL70002.1"
                     /translation="CGFCRKMREQVFPTREFQEAASSFVLARVNADEDLSARKYNVEG
                     LPTLLMLDHNGYLIGRSDGFTEPERLVRLMKEALGRAGTQAKVEKDARERPGVNSSYR
                     AGLYYASINDSPRARSHFLNAWQAGKAKPDPQSFDALYNAAVSSMEMKDYSGAVQLWS
                     QYVDLYPSRDRDFAYARYFRGLALRSLGKKEEAKADMAYASNNLPPGEDKNAAIRMAG
                     K"
BASE COUNT          750 a          788 c          819 g          865 t
ORIGIN      
        1 tgtatacgaa ctctcctacg actttgagtc cggatgatac ggccggtgtg agggcagtgt
       61 atggtccgta gcctgtgcgt ggtcgcctta tcattgttat gcgcatgcag caatgtgaaa
      121 ttctgggaaa aggaaaaaga ggacaataca aaagaactca tccttcttct ggctgcggct
      181 tatctggcaa atccttgtaa cttcaccgcg tctcctgtgc ttacgagtgc acaaccttct
      241 ggaaccatga ccggagtggc aggaagcatc aagggaaaac tgcttaccag ggcgggcacc
      301 cctgccatca gcgcgctcgt catcgcagag gacaatgcgg ccattggagt gaactactgg
      361 gccactcatt caagcatcaa tcgagatgga tcatttcaga tcagcgggat ccctggcgga
      421 accactgtga agctttcctt cgaatcgatt ctatctgatt acaacgggcg catagacggc
      481 cacattgatt gtttcaaaac accttctacg ttcacggctg gctgggtcac gggcggcact
      541 ctcactacta ctcgagcatc cgggcagaac ttctccatag tcaacgcaac gactctggat
      601 gccggaacca tctacctggt gaactgatgc cggagttgcc ggatctagtc tacgtcgctc
      661 gcggcctgca tgattttatt tctggccgca tcattgaaca agtggaagtg cgagaaccca
      721 ttgtggttcg caatttgacg ggagagcctt ttggagcctg cgtgaccggc agaggaattt
      781 tgaacgtcag gcgacatgga cctttccttg tatttgaact tacgggacac aggatgatcg
      841 tggctcatcc aatgctcgcc ggtgccttta gcattgaacc aaagtccgcc ggtctcatca
      901 ctttcatttt caaagaaggg aaactatcct atctcgatgc aaagaagatg ggaaagattt
      961 atgtcgtgga cccgggtcag gaagcgcaga ttccaaggtt taccacacag ggagtggaca
     1021 tcacttcccc tgagtttacg gaagatgttt ttcttggact cattcgcaca agccgccgtc
     1081 aggtgcgtgc ctttctcatg gaacaggaac agatcagtgc catcggcaat gcatatgcgg
     1141 acgaaattct ttttgaagct agaattcatc caaaaacgtt ttgccatcag cttggcccgg
     1201 atgagcaaaa agttctatat gattctattc gaacggtcat cgatcgtgga atcaaagaag
     1261 tgcagggagc caatgccggc ctgcatgtaa agcaccgaga tcacatgctc gtgcgaaata
     1321 aaaagaatga accttgtccg cgctgtggtg caaagatcag gagagagagt gttctgggct
     1381 acgatacttt tttctgcccc gtctgtcagc cagcgaagag aaagctgttc attgactgga
     1441 aataggttcc ctttttgcaa gagagacaat cgagtccgtt gtagatagga gcttttccat
     1501 gtcggctctg gttccgattg tgattcgtat ccactctttg agcttttcgg atgaccagta
     1561 tcgtaccaaa attttatttt ccagaaggag tctgtagtac gcaccggcag ctgtttcccc
     1621 tctttgcgag agtgctctta gatctgcagg tggtcttgtg aaaatgaagt tggtgctcga
     1681 gggcagtgtt tcaaaaccca gcctgtccag tccctctgtg agaaagtttc gattatttac
     1741 tatttcatct cttctttttt caaattcgga tttgtccagt actgcggcca gcgcggctgc
     1801 ctgggccagc ctggaaacgt tgtaggaatc tttgattttc tcaagctccg caatcaagga
     1861 ttcttccgcg cacaaccagc cgattcgtgc tccggcaaga gagagagctt tggacgcagt
     1921 gccgctgacc agaagtcgcg ggtaggtaga tgttcccgca ttcttaaaaa cagttacatt
     1981 tccaaagtca gcgtatgctt catctaccag ggtgagagat gggttgcgcg aggtgaagtc
     2041 caggatatcc ttttctgatt ccgcgattcc agtgggtgca tttggatttg caaatgcagc
     2101 cagggcgatt cctcctttct ggattttttg aagcatggct tcaaaatcaa gagtccagtc
     2161 ttcgcgcacc tggatttcaa tgcacgagct gtgcgaaagt gcggcgatca ccggatagag
     2221 gctgtacgta ggatagggcg caatcatggc tttatcctgt ccaagaactg cacgaaatag
     2281 aatgcttaag atttcatccg atccatttcc aaccaggaag cattccggcg gccttccgta
     2341 ttttgcagcg agtgcttctc ttaatctttg cgaaacaggg tttggatact tctgtagaag
     2401 cttgccgtca atttcctgaa ccagagcgtt tcgcacggat gcgggaatgg ggaacggatt
     2461 ttcattcgta ttgagtttga tgacttccgg gtcctctgtt tgaaggccag gaacatatcc
     2521 gtctaaatgg cggatgtgtg agtgaacaaa ttcagatggt ttcacttccc ggccattcgg
     2581 attgccgcgt tcttatcttc tccaggcggc agattgtttg atgcgtatgc catgtccgct
     2641 tttgcctctt cttttttccc aaggcttcgt agagcgagtc ctctaaaata gcgcgcatag
     2701 gcaaagtccc tatctctgga tgggtagaga tctacatact gtgaccagag ctgcaccgca
     2761 cccgaatagt ctttcatttc catagaactg actgctgcgt tgtaaagtgc gtcaaaggat
     2821 tgcggatctg gttttgcttt gcctgcctgc cacgcattca ggaaatgaga gcgcgctctt
     2881 ggagaatcat tgatgctcgc gtagtagagt cccgcgcgat aagaggaatt cactcctggc
     2941 ctttcgcgtg catctttttc tacttttgcc tgtgtgcccg cccgaccgag tgcttctttc
     3001 atcaatcgca ccagtctttc cggttcagtg aatccatctg atcttccaat cagatatcca
     3061 ttgtggtcga gcatgagcag agtgggcagg ccctccacgt tgtatttgcg cgcagacaaa
     3121 tcttcatccg cattcacgcg ggccagtaca aaagacgatg ctgcctcctg gaattctctg
     3181 gttgggaaaa cctgttcgcg cattttgcgg caaaagccgc ac
//
LOCUS       DAORFF010000469         3209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_19603_length_3209_cov_3.594483, whole genome shotgun sequence.
ACCESSION   DAORFF010000469 DAORFF010000000
VERSION     DAORFF010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19603_length_3209_cov_3.594483"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKK76_15845"
     CDS             complement(<1..514)
                     /locus_tag="PKK76_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HNL70003.1"
                     /translation="MSMEDLPEIQKIISIGKANGEITYDEINEYLPEKIVNSEWIEDV
                     FTLLNQMGIEVVEEYSSRPQEEVVKKEPPAKARKKKDAPANSSDDPIRLYLKEIGRVS
                     LISGDKEVFLARRIENGENIIEETILESSLLRANFMKLLPKIRSRKIKIIDVVRANKA
                     YYMSTTELEKL"
     gene            complement(521..2380)
                     /gene="dnaG"
                     /locus_tag="PKK76_15850"
     CDS             complement(521..2380)
                     /gene="dnaG"
                     /locus_tag="PKK76_15850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNL70004.1"
                     /translation="MNDDLKQRIIAAIPIESYIGRYVKLKKTGKGFTGLCPFHQERSP
                     SFHVRPDTGRFHCFGCGKDGDLFTFVMEKESLSFVQALDELARYAGIERTPGKEDPLK
                     RYYDLNEQALRLFRSHLKTDDVVQKYMESRSIHEDTAARFEMGSSPDSWNWLAESLDR
                     EGIRGKEELLKEMGLIRAKEEDRRTYDFFRGRVMFPIRDAVGRLCGFGGRALPGFDDR
                     AKYINSQDSRVFHKQELLYGLYQSLKEIRPSREAILVEGYLDVIGLSQAGLSRAVAPL
                     GTAFTESQLRLIEKYADTLVCMMDGDKAGRAAALKAVRLALEKGKLRVRVLLLPEGMD
                     AFDLSVKTDAATILSLLALSFPGERYLIVETMFPGTAVIPAAEPGSEGLLRFGEHVRR
                     EYERKRMELDASSKRDGLARLDELVKTMPPELGQILKQEGSAIIGVSPDRARSPMQKR
                     PAQGRTVMAPPLSNIAKCEREIVAIFMFHPTALVDLREEMDSFSFQDEPSEIMVRYLD
                     TRAISGNLWTPETVFSDQLPDSIVSIFHGLLLSRAPSEDPVGVVRDLLVEHRIRSLEK
                     EFEDLDLQLAGADPVMEFPLLSKKNEIVLELQTLRSKKMRLSGVETPQGRSEV"
     gene            complement(2389..2844)
                     /locus_tag="PKK76_15855"
     CDS             complement(2389..2844)
                     /locus_tag="PKK76_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNL70005.1"
                     /translation="MPTLQEKIGEDIKDAMRKKEEVRLGTLRMLKSELQYEMTKTGAS
                     TLEDDLVMTLIRRAIKKRQEAKDQFEKGGRPEMAEKEAAEMSVLETYLPASISEDVVR
                     AKAQEIIAEVKPAGPQDFGKVMGKVMAAFKGQNIDGNTVNRIVRSLLGN"
     gene            complement(2845..3054)
                     /gene="rpsU"
                     /locus_tag="PKK76_15860"
     CDS             complement(2845..3054)
                     /gene="rpsU"
                     /locus_tag="PKK76_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002104827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNL70006.1"
                     /translation="MIGVILKEGESIESALKRFKRECVNAGIQSEVKRREYFEKPSER
                     RKRKHDAAVRKREKKRIIMLRKDKS"
BASE COUNT          714 a          848 c          766 g          881 t
ORIGIN      
        1 cgagcttttc cagttctgta gtggacatgt agtacgcttt gtttgcgcgc acaacatcta
       61 tgattttgat tttgcgagaa cgaatcttgg gcagaagctt catgaagttc gcacgaagca
      121 aagacgattc gagtatggtt tcttctatga tgttttcgcc attttcaatg cggcgtgcca
      181 ggaatacttc tttgtctccc gaaatcagag aaacgcggcc aatctctttg agatagagac
      241 gaatcggatc gtcgcttgaa ttggcgggcg cgtctttctt ctttcgcgct ttggcaggtg
      301 gttccttctt gaccacttct tcctgcggtc tgctggagta ttcctccaca acctcgatgc
      361 ccatctggtt gaggagtgta aacacgtcct caatccactc cgagtttacg atcttctcgg
      421 ggaggtactc attgatttcg tcgtatgtga tctctccgtt ggctttgcca attgagatga
      481 tcttttgaat ttccggtagg tcttccattg acatggccgg ttatacctcg ctcctgccct
      541 ggggtgtttc cactccagat agcctcattt ttttggaccg caaagtctgc aattccagga
      601 caatctcatt cttctttgag agcagcggga attccattac aggatcggct cccgccagct
      661 gcaaatccag atcctcgaac tccttttcga gacttcgaat ccggtgttct acaagcaaat
      721 cccgcacgac tccaacaggg tcctctgatg gggcgcgtga gagtaaaagc ccatgaaaga
      781 ttgagacgat tgaatcggga agctggtctg aaaaaacagt ctccggcgtc caaagattcc
      841 cagaaatagc tctggtatct agataacgaa ccatgatctc tgacggctcg tcctgaaagg
      901 aaaaagaatc catttcctcg cgtaaatcca cgagggcggt tggatgaaac atgaagatgg
      961 caacgatctc ccgctcgcac tttgcaatgt ttgagagcgg aggggccatc acagtgcgac
     1021 cctgagcagg ccttttctgc atgggagacc tggcgcgatc aggcgatact ccaatgatcg
     1081 cgcttccttc ctgtttcaaa atctgcccga gttctggcgg catggtcttc accagctcat
     1141 ccaatctggc caggccgtct cttttagaag acgcatcgag ttccattctc ttgcgctcgt
     1201 attcgcggcg aacatgctcc ccaaatctaa gaagaccttc tgaaccaggt tccgctgcag
     1261 gaatgactgc ggtgcccgga aacatggtct ctacgatcag atatctctct cctggaaaag
     1321 aaagtgcaag gagagacaga atggtagcag catctgtttt tacagataga tcaaatgcat
     1381 ccatgccctc tggaagtaga agcactcgca cacgcagttt ccctttttca agtgcaaggc
     1441 gaactgcttt gagtgcagcc gctctacccg ccttgtctcc gtccatcatg cagacgagtg
     1501 tatctgcgta tttttcaatg aggcgaagct ggctttccgt aaatgcagtt ccaagcggag
     1561 ccacagcgcg agaaagacct gcctgcgaaa gaccaatcac atccaggtag ccttccacaa
     1621 gaattgcttc ccgcgaaggc ctgatttctt tgagagattg atacaaaccg tacagcagtt
     1681 cctgcttgtg aaatacgcgc gaatcctggg agttaatgta tttggcgcga tcatcaaaac
     1741 cagggagagc tcttccgccg aatccgcaca gcctgcccac tgcatcgcga atgggaaaca
     1801 tgacgcggcc gcgaaagaaa tcatacgtgc ggcgatcttc ttctttcgcg cgaatgagtc
     1861 ccatttcctt gagcagttct tctttgccgc gaattccttc gcggtcgagg ctttcagcaa
     1921 gccagttcca cgaatcagga gaggagccca tttcaaaacg agctgccgtg tcttcatgga
     1981 tcgaccgcga ctccatgtat ttctgcacca catcgtctgt tttcaagtga gagcgaaaca
     2041 ggcgaagggc ttgctcgttt aaatcataat agcgtttgag tggatcttct tttccggggg
     2101 tgcgttcaat tcccgcataa cgggccagtt cgtcgagagc ctgcacaaaa gacagagact
     2161 ctttttccat gacgaaagtg aacagatccc cgtctttgcc gcatccaaag cagtgaaatc
     2221 gcccagtgtc aggacgcaca tgaaaggacg gacttctttc ctgatgaaaa ggacaaaggc
     2281 ccgtaaaccc tttcccggtt ttcttgagct tcacataacg gccaatatac gattctatag
     2341 gaatggccgc gatgattcgc tgctttaagt cgtcgttcaa gcgacctctc agttcccgag
     2401 cagggagcgc acgatgcgat ttacagtgtt gccgtcaatg ttctggcctt tgaaggcggc
     2461 catgaccttc cccattactt tgccaaaatc ctgggggcct gccggtttta cttctgcgat
     2521 gatttcctgc gccttcgcgc gaactacgtc ttcagaaata cttgccggca aataagtttc
     2581 aagaacggac atttctgccg cttctttttc cgccatctcg ggacgcccgc ccttctcaaa
     2641 ctggtctttt gcttcctgac gtttcttaat ggcgcggcga atgagcgtca tgaccaggtc
     2701 atcctccaac gtggaggcgc ccgtcttggt catttcatac tggagttcgc ttttgagcat
     2761 tcggagggtg ccgagtcgca cttcctcctt ctttctcatt gcgtccttta tatcttcgcc
     2821 gattttttcc tggagagtgg gcattcagga tttgtctttt ctgagcatga tgatgcgctt
     2881 tttctcccgc ttgcgtactg cggcgtcatg cttgcgcttg cgtctttctg acggcttttc
     2941 gaaatattcg cgtctcttta cttcggactg gattccagcg ttgacgcatt ctcttttgaa
     3001 ccgcttcagt gcggattcaa tagattcacc ttctttgagt ataacaccaa tcatttgtta
     3061 gaacgttttc tccatattaa gttgcctgca ggggaccaaa tcaccacatt tttgtaaggc
     3121 aggagttccg taaacccctt ctttgagccg ctttcttcgc aaaaggccaa tcgggcgact
     3181 gcccgttttg accatggact ctgagggtt
//
LOCUS       DAORFF010000470         3207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_19626_length_3207_cov_3.448921, whole genome shotgun sequence.
ACCESSION   DAORFF010000470 DAORFF010000000
VERSION     DAORFF010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19626_length_3207_cov_3.448921"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="PKK76_15865"
     CDS             <1..1580
                     /locus_tag="PKK76_15865"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNL70007.1"
                     /translation="WRNDSPVEMYFTSDAKARGEGYDGLNIYYTRFRDDRWSKPIQID
                     TLCSNFDDRMPWISKDGLRIVFSSNRPGGYGGYDLYFADRDPKTGKWSLPVNAGPKIN
                     SEADEISPSFSPDRGMLFFSSNREGGLGHFDIYVSRFREDSFEAAENLGRPFNSERDD
                     EYFTITEDRLWAYFASERQHVDAAGRLDLYRTALPDWLRDPVDVAFSMLIMDASTRLP
                     LGLEATVKIQWEKDSIVRHSKPFNKDPKDLEKSNFEQSLVTGRQYRVVISAPGFYPQE
                     ILLDYRGSVPSSKKDHRIVYLEPVKQTNPEESRIVRGRIVDAATDLPLSSTRLRKVTA
                     GKDAEKIETIPTDNKGHFELRLTKGEKFTLRADAPGYTQTTESFEEKETLKEIVLRLK
                     PSSGDPCKGDLPECIENLRIFFALGASDLSPSEKRKLDAVIRILKKNPDLRIEIRGHT
                     DRTFRGPEQNSMAYNQKLSEIRSASVRRALVEGGIDTGRLITVGKSYLEPEESETSAA
                     GRDRNRRVEFRRLSLDK"
     gene            1630..2760
                     /locus_tag="PKK76_15870"
     CDS             1630..2760
                     /locus_tag="PKK76_15870"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HNL70008.1"
                     /translation="MEILRDKKRDWDLLREADKIQYILRDKLRSKPFYLKGSEPPVEF
                     IMGNILPEGIEFFTIPEQSAQTQVTLYLTLNRHIEVDFEVKNIQDDRWLLMPTLARIG
                     KVVRAHPRFAMAEGSVYATNFKVSKNELGADIARLQIAAQVVLRDQEKQLAEEFPGLR
                     MFLTADRDRPLGLRPIEHMTETLVIKDTNDPHAYEIPVGDTTYFKLLGKTFEQVRLKL
                     GDTASLVIYPIALEEKGKKYLIAHMYLPLKHTGTDFDTLLKTLSEKAELIIERIREVN
                     MVTIKEKQKVRDISLGGVGIEIDNSDLKKYLPVSDRITFDLIFKMQAPLRFQGKICHV
                     AAVGDETLLAGIDIEGTGHTEYRKSNQERLGTLIQLIRSESK"
     gene            2760..>3207
                     /locus_tag="PKK76_15875"
     CDS             2760..>3207
                     /locus_tag="PKK76_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNL70009.1"
                     /translation="MAGPYVGTRDFYPEDMQFRNAMFAVIRSVCESYGYLEYSAPLLE
                     PISLYQSKSSEEIVNDQVYRFTDRGDREVAIRPEMTPTMARMVSARGMELIRPVRWYS
                     IANFMRYERPGKGRLREFYQLNADLLGPATPAADAEILSLLIDILTA"
BASE COUNT          880 a          748 c          816 g          763 t
ORIGIN      
        1 tctggcgcaa cgacagtcct gttgaaatgt atttcacgtc ggatgcgaaa gcgcgcgggg
       61 aaggttacga cgggctcaat atttattaca ctcgctttcg cgacgatcgc tggtccaaac
      121 caatccagat agatactctc tgttctaatt ttgacgatcg gatgccctgg atttctaaag
      181 acggccttcg cattgtgttt tcttcgaacc ggccgggcgg gtatggcggc tacgatttgt
      241 atttcgcaga tcgcgatcca aaaacaggca agtggtcttt gcccgtgaac gccggaccga
      301 agatcaactc tgaggcggat gagatttcac catccttttc tccagatcgc gggatgcttt
      361 tcttcagttc gaatcgagaa ggtggtctgg ggcattttga tatttacgta agtcgtttca
      421 gagaagattc ctttgaagcg gcggaaaatc tgggtcgtcc ttttaattct gaaagagacg
      481 atgaatactt caccataaca gaagacagac tctgggccta tttcgcaagc gagcgccagc
      541 acgtagatgc agccggcaga ctggatttgt atcgcactgc tctgccggac tggcttcgcg
      601 atccagtgga tgttgcattc tccatgctca tcatggatgc atctaccaga ttgcctcttg
      661 gtctggaagc aaccgtgaag attcaatggg agaaagactc catagtccgt catagcaagc
      721 cattcaacaa agatccaaag gatttagaaa agagtaattt tgaacagtct ctcgttactg
      781 gaaggcagta tagagttgta atctccgcac ctgggtttta cccacaggag attcttctag
      841 actatcgcgg aagtgttcca tccagcaaaa aggatcacag aatcgtttat ctggaacctg
      901 tgaagcaaac caatccagaa gaatccagaa ttgtccgcgg gcgaattgtg gatgctgcta
      961 cagatctacc tctatcttct acgcggcttc gcaaagtaac ggcaggaaaa gacgcagaaa
     1021 aaatagaaac gattccaaca gacaacaaag gacattttga gctgcgtctt acgaaggggg
     1081 agaagttcac tttgcgagcg gatgctccgg gctacacaca aacaactgaa tcgtttgagg
     1141 aaaaagagac tctcaaagaa attgtactgc gtctcaaacc atcttctgga gatccatgca
     1201 agggagacct gccggaatgc attgagaatc ttcgaatatt ctttgcactt ggtgcatccg
     1261 atctttcccc gagtgaaaag agaaagctcg atgcggtgat tcgcattttg aaaaagaatc
     1321 cagatcttag aatagagatt cgcggacaca cggaccgcac tttccgcgga cccgaacaga
     1381 attcaatggc atacaatcaa aagttatcgg agattcgttc cgcatctgtt cgtcgcgctc
     1441 ttgtagaggg tggaattgac accgggcgtc tgataacagt gggcaagagc tatctcgagc
     1501 cagaagagtc agaaacatca gctgccggac gtgacagaaa caggcgcgtc gagttcagga
     1561 gattaagtct tgataaataa ctctgtgcaa gcactatcgc cgcaggccac ggattcgtct
     1621 aattgagcta tggagatact tcgcgacaaa aaacgtgact gggatttgct gcgggaagca
     1681 gataaaatcc agtatatcct ccgagacaag ctgagaagca aacccttcta tttgaaagga
     1741 tctgagccac ccgtcgaatt catcatgggc aacatcttgc ctgaaggaat tgagtttttt
     1801 accattcccg agcaatcggc tcagacccag gtcactctgt atcttactct caatcgccac
     1861 attgaagtgg acttcgaagt aaaaaacatt caggacgatc gctggcttct catgccaaca
     1921 cttgctcgta ttggcaaagt ggtgcgcgcg catccgcgtt tcgccatggc agaaggaagt
     1981 gtatacgcta cgaatttcaa agtaagcaag aatgagctgg gagctgacat tgcaagactc
     2041 cagatcgcag cgcaggttgt gctacgcgat caggagaagc agcttgcgga ggaattccca
     2101 ggcctacgta tgtttctgac tgcggacaga gatcggccat tggggcttcg ccccattgag
     2161 cacatgacgg aaactctggt catcaaggat accaatgatc ctcacgcata cgaaatcccg
     2221 gtaggggaca caacgtactt caagctactc ggaaagacgt tcgaacaggt tcgtctcaag
     2281 ctaggagaca ccgcgtccct tgtaatctat ccaattgccc tggaagaaaa gggaaagaaa
     2341 tatctcatag cacacatgta tctccctctg aagcatactg gaaccgattt tgatacgcta
     2401 ctcaagaccc tcagcgaaaa agctgaattg atcattgaac gcattcgcga agtgaacatg
     2461 gtcaccatca aggaaaagca aaaagtccgt gatatctcgc tgggaggagt gggcattgag
     2521 atcgacaaca gcgatttgaa gaaatacctc ccggtcagtg atcgcataac gtttgatttg
     2581 atttttaaga tgcaggcgcc gctcagattc cagggaaaga tttgccacgt ggcagcagta
     2641 ggggatgaga cattgcttgc tggaattgac attgagggaa cggggcacac agaatacagg
     2701 aagtcgaatc aggaacgact gggcacattg atccagctga ttcgttcgga atcgaagtaa
     2761 tggctgggcc ctatgtggga acgcgggatt tctatcccga ggacatgcaa tttagaaatg
     2821 caatgtttgc tgtgattcgt tctgtgtgcg aatcgtacgg ctatctggaa tattcggctc
     2881 ctcttcttga gccaatcagc ctctaccagt ctaaatcatc ggaagaaatc gtaaacgacc
     2941 aggtgtatcg ctttacggat cgcggagaca gagaagtcgc catccgtcct gagatgactc
     3001 ctacgatggc tcgcatggtt tccgcacgcg gcatggaatt gattcgtcct gtgcgctggt
     3061 acagcatcgc gaacttcatg cgatacgaaa gacctggcaa gggcaggctg cgcgaatttt
     3121 atcagttgaa tgcggacttg cttggtccgg ccacgcccgc ggcggacgct gaaatcctga
     3181 gtctgttaat tgacatattg actgcgt
//
LOCUS       DAORFF010000471         3201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_19688_length_3201_cov_3.480610, whole genome shotgun sequence.
ACCESSION   DAORFF010000471 DAORFF010000000
VERSION     DAORFF010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19688_length_3201_cov_3.480610"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..718
                     /locus_tag="PKK76_15880"
     CDS             125..718
                     /locus_tag="PKK76_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNL70010.1"
                     /translation="MSEVQKDSRQKLIDSAVELFYRDGYRTGLSDILRTAGLNKSTFY
                     HYFESKEALVLEALTLRSESEHKFLRDLAAKKKDLGTFLSSWVQTVTRAARSGKFVGC
                     PLAAFAYQLSEAEAKVYGDRLALTTHRWLGDVVQLIESLKKAGSLSATLDAEALGMRI
                     LTAYEGAVTMWRLTRSDHHWTHLKQEFARIARDAVET"
     gene            883..2145
                     /locus_tag="PKK76_15885"
     CDS             883..2145
                     /locus_tag="PKK76_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70011.1"
                     /translation="MKRILGILALFALVTPAFAQTSLSSLFQKPGAIGDSLSQGFFGV
                     TVEQKTQDWAYPVLVSKQAGSNLSYNVLKGPYVNLEDVLKGDCGPICIASAIIGGNGS
                     TVALPTHAGITGADYTDALYKSGQCQDIRATKQVKEWYWATWYWYTYRWITVADCQEP
                     DKFFRFGLRDAGTQIQIMEKVRPTFAFAAVGANHVLCTALGTSLACLDEPRFKRDVAE
                     SFRRLKAMGSLKGGVIFSVPDVTTIAYLERWNDPSGRANYSGLKPFYRGTVSSPDQVL
                     DAGEIATVQKFLAMENTELRNQATGANFAFTDAKALFDDIKANGRVLKSSSGYSPGNG
                     QAMWPLPGRPGLFGLDGVHPNRYGHSIMANELILAINKQYGVAIPKVDEYSAWYYDSL
                     NRSPVDLKNFLTNSIFGQVITWIVDTFM"
     gene            2238..2891
                     /locus_tag="PKK76_15890"
     CDS             2238..2891
                     /locus_tag="PKK76_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70012.1"
                     /translation="MNRNAIIALVALVGVILVAVIIYMATGRGNQQAGTPGGPGGETV
                     FKDLYPDAPNPAEDPELAEQIKNLWPDVAKAKPDRDAIRREWQDFAKKYPNNIYIPDE
                     FKGTLSEVEAQDRRKTLDTVTAVETKFANMRAEAKKANPGQDGPPAPPNSNVAPAEQR
                     VYFDYKIREIESRIQLVEYMLSNGSPDAAQKASAQKEMAQWSKELENYKKLKQSIPN"
     gene            2891..>3201
                     /locus_tag="PKK76_15895"
     CDS             2891..>3201
                     /locus_tag="PKK76_15895"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNL70013.1"
                     /translation="MCATTVFRFRVALIVFALAGACGPPRPIDLPENKEPPVPPLSEV
                     MAGLKSAVWSERSEAVLAAGHGHYKETIPLLLSLLSSDPNPAVRSTAALVLADLGEQR
                     AA"
BASE COUNT          811 a          788 c          853 g          749 t
ORIGIN      
        1 tagtgcttgc cattgtttgg gtcatattct ctcctgtacc aaccggtcgg tccacgatgg
       61 ggacgtgttc tgaatctggc aagaagaaag ttgtgcgggt gagagggaag ggagagatgc
      121 gcaggtgagc gaagtgcaga aggacagcag gcagaagctg atcgattcgg ccgtggagct
      181 attctatagg gacggctatc gcactgggct ttcggatata ttgcgaacgg cagggctcaa
      241 caagtctact ttctatcact atttcgaatc caaagaggcg ctcgtgctgg aggctcttac
      301 cctgaggagc gaatcggagc acaaattcct gagagatctg gccgccaaga aaaaggatct
      361 gggaactttc ctcagtagtt gggtccaaac tgtgaccagg gctgcccggt ctggaaagtt
      421 tgttggatgt cccctggccg cgttcgctta tcagctttcc gaggcagagg cgaaagtgta
      481 cggggacagg ctcgctctca cgactcatcg ttggctcgga gatgtagtcc agctgattga
      541 atctttgaag aaggcaggca gcctttcggc cactctggat gcggaggctc tgggaatgcg
      601 aatcctgact gcttatgagg gagctgtgac catgtggaga ctgaccagat cggaccacca
      661 ctggactcat ttgaagcagg agttcgctcg gatcgcacgc gatgccgtgg agacataatc
      721 ggtttaacgt gaaacggcga ataacgttgg aatttcgcat ttcagtctta tgtctcttat
      781 gatttcattt tttgacacag tgtcattgaa aaagaattga tgatccggat aggggatgct
      841 agaagtgggc atccgatccg ttggatcgtg tggggggaaa atttgaaaag aatcctgggc
      901 attcttgcgc tctttgcgct ggtcacacca gccttcgcgc aaacatcatt gtcatcgctc
      961 tttcagaagc caggagccat tggagactct ctgtctcaag gtttcttcgg tgtaactgta
     1021 gagcaaaaaa ctcaagactg ggcctatcca gttctcgttt ctaagcaggc aggctccaat
     1081 ctgtcttaca acgttctgaa aggtccttat gtaaacctgg aagacgtttt gaaaggggat
     1141 tgcggtccca tctgcatcgc atctgcgatc ataggtggaa acggaagcac tgtcgctctc
     1201 ccgactcatg ccgggatcac aggagcagac tacacagacg ctctctataa aagcggtcag
     1261 tgccaggaca tccgtgctac caagcaggta aaagaatggt actgggccac ctggtactgg
     1321 tacacatatc gctggatcac agtggcggat tgccaggaac cagacaagtt cttccgcttt
     1381 ggtctgcgcg atgcaggcac tcaaatccag attatggaaa aagtgcgtcc tacattcgca
     1441 ttcgcggcag tgggcgctaa ccacgtactc tgtactgccc tcgggacaag ccttgcttgt
     1501 ctggacgagc cacgcttcaa gcgcgacgtt gcagaatcat ttagacgcct caaggcgatg
     1561 ggttctctga aaggtggagt gattttctct gttccagacg tgactacaat tgcgtacctc
     1621 gaaagatgga atgatccttc cggccgcgcg aattactccg gtctcaaacc tttctatcgc
     1681 ggaactgttt ccagtccgga tcaggttctt gacgcaggag aaatcgcgac tgttcagaag
     1741 ttccttgcta tggaaaacac agagcttcgc aatcaggcaa ctggtgcgaa tttcgcattc
     1801 acggatgcga aggctctctt cgacgacatc aaagcaaatg gtcgggtgct caaaagctca
     1861 agcggctact ctccaggaaa cggacaggcc atgtggccac ttcctggtag accaggtctc
     1921 tttggtctag atggagttca tccaaatcgt tacggtcatt ccattatggc aaacgagttg
     1981 attctcgcga tcaacaagca atacggagtg gcaattccta aagtggatga atactctgca
     2041 tggtactacg attctctcaa tcgcagccct gtggatctca agaacttcct tacgaattct
     2101 atcttcggtc aggtgattac ctggatcgta gacacattca tgtgatctga aaattgatca
     2161 cagaaaaacc cggggccaac gctccgggtt ttttttcgct cttgtcaaca aagcactgtt
     2221 ccttccaatg aggtaatatg aatcgcaacg caatcattgc actcgtggct ctcgtaggag
     2281 tcatccttgt cgcagtcatc atctatatgg ctacaggccg cggaaatcaa caggccggaa
     2341 ctcctggtgg gccaggcgga gagactgtat ttaaagatct ttatcctgac gcgcccaacc
     2401 ctgcagaaga tccagaactc gcagaacaga ttaagaatct gtggccggat gttgcgaaag
     2461 caaaaccgga tcgtgacgcg attcgccgcg aatggcagga ctttgcaaaa aaatatccca
     2521 acaatatcta cataccggat gagttcaaag gaacgttaag cgaagttgaa gcacaggatc
     2581 gcagaaagac tctcgataca gtgactgcag tggaaactaa attcgcaaat atgcgagcgg
     2641 aagcaaagaa agcgaatcca ggtcaggacg gaccacccgc acctccgaat tccaatgtag
     2701 cgccagcaga gcaaagagtg tatttcgatt ataagatccg cgaaattgaa tcgcgcattc
     2761 agcttgtaga gtacatgctt tccaatggca gcccggatgc ggcgcagaag gcttccgcgc
     2821 aaaaagaaat ggcacagtgg agcaaggagc ttgagaatta taagaagctc aagcaatcca
     2881 ttccaaactg atgtgcgcaa ccacagtgtt tcgcttcaga gttgcactga tcgtcttcgc
     2941 attggctggg gcctgtggac ctcccaggcc catcgatctg cctgaaaaca aggaaccgcc
     3001 ggtgccacca ctgagcgaag tgatggccgg cctcaaatcc gcggtctggt ctgagagatc
     3061 ggaggcggtt ttggccgcag gccacgggca ttacaaagaa acgattccgc tcttgctttc
     3121 tttgctcagc tcggatccga atccggccgt ccgctctaca gccgctctcg ttctcgcaga
     3181 tctgggagaa cagcgtgccg c
//
LOCUS       DAORFF010000472         3195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_19742_length_3195_cov_3.221656, whole genome shotgun sequence.
ACCESSION   DAORFF010000472 DAORFF010000000
VERSION     DAORFF010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19742_length_3195_cov_3.221656"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PKK76_15900"
     CDS             complement(<1..520)
                     /locus_tag="PKK76_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNL70014.1"
                     /translation="MENANLEFDYVIIGSGFGGSVSAMRLAQKGYSVAVLEAGKRWEG
                     KDFPKTNWSLRKFLWMPKAFCYGIQRINVLNDVMILSGAGVGGGSLVYANTLYVPKKE
                     VFEYPLMKKMGGQRHMLPFFQIAQKMLGVITNPALTEVDELMKQTAAELGKGDTFTPT
                     PVGIYFGEAGKTV"
     gene            610..1074
                     /locus_tag="PKK76_15905"
     CDS             610..1074
                     /locus_tag="PKK76_15905"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HNL70015.1"
                     /translation="MKVGIQTGSLKGRAAGSARTSTGESTTAEKNSFLDLLTEVLPAS
                     TPERASLQNLWSQMPQAERDLIDNPSKDNLAAYQDLVRAIARQTLDEAMKVEKRTRPA
                     TFSRAQNELTTVRIIDEKLHRMALVMLSPENSAFSMMKSLAEIRGFLMDMRR"
     gene            complement(1082..1768)
                     /locus_tag="PKK76_15910"
     CDS             complement(1082..1768)
                     /locus_tag="PKK76_15910"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNL70016.1"
                     /translation="MNEPSQLLYFGDPMCSWCYGFAPVLLRLKEDLEGRLPIHCVLGG
                     LRPGPHAEKMDEKLKRFLRHHWEEVHRTTGQEFGYKIFDRDDFVYDTEPSCRAVAIVR
                     KLKPDAVIDFFAQVQTAFYRDSKDTTMLETYIPLAAEIGVSPEEFTALWNSEDMKAET
                     WKDFEFAASAGVRGFPTLIYIGEGRGQIICRGYLPYEDVRERVETVLTARKPVLAPTQ
                     EACDIEKGIC"
     gene            1849..2862
                     /locus_tag="PKK76_15915"
     CDS             1849..2862
                     /locus_tag="PKK76_15915"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNL70017.1"
                     /translation="MEGESFYSLTPESILASVEAAGLPVTGRCLQRNSLENRVYEVEI
                     DIESENKSERFRIAKFYRPGRWTREQILEEHQFISDLRAQEIPCAPPIALPGGSTLGK
                     TESGIFFSLFERVGGRQLDELGGTEREQLGRLLARVHNVGAASKSVHRIKLNEETYGL
                     QNLDYLLESESIPEHMEERYAQLVEDICEHTANLREELSAPNEFLPALEEIRLHGDCH
                     GGNILWSNAGPFLIDFDDMVSGPPVQDLWLLASDSEELEAIVNGYEQMRTFDRRSLKL
                     VEHLRALRYIHFSAWIARRWEDPSFPGAFPAFGTEQYWATQIDDLVEQLERIETTQSP
                     FSD"
     gene            2899..>3195
                     /locus_tag="PKK76_15920"
     CDS             2899..>3195
                     /locus_tag="PKK76_15920"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNL70018.1"
                     /translation="MIKTPNVFNLIYSVVLWAILLTVTLFFGISVMPFALFRARKAVH
                     FGARMWGKSLAFLSRMTIQVKNKEKLYRGGPVIYLANHQSMFDIAVFYSFLDTEF"
BASE COUNT          815 a          863 c          772 g          745 t
ORIGIN      
        1 tcacagtctt gcccgcttcc ccgaagtaaa ttccaacagg agtgggtgta aacgtatctc
       61 cttttccaag ctccgccgca gtctgcttca tgagttcgtc cacttctgtg agcgcaggat
      121 tggtgatcac tccgagcatc ttttgtgcga tttgaaagaa tggcaacatg tgacgctgcc
      181 cgcccatctt cttcatcaaa ggatattcga agacttcctt cttgggcaca tatagagtgt
      241 tggcatacac cagactgcct ccgccgactc cggcgccgct caggatcatg acgtcgttga
      301 gaacattgat gcgctggatt ccgtagcaga acgctttggg catccagagg aatttgcgaa
      361 ggctccagtt tgttttgggg aagtctttgc cttcccagcg cttgcccgct tcgagcaccg
      421 ccacagagta gcccttctgg gccagtcgca tcgcggaaac gcttcctcca aaacccgaac
      481 caatgattac gtagtcgaat tctaaattcg cattttccat cggcagagtc caaactcggc
      541 ggccggagtg tccatccaaa agcagaaaag ttctactgca ttcgagcaat tctgccgatc
      601 ttcgaaccag tgaaagtagg cattcaaacc ggatccctca aagggcgagc ggccggctcc
      661 gcgcgcacaa gcacaggcga atcgacgact gcagagaaaa acagcttcct cgatttgctg
      721 acggaagtgt tgcccgcgag cactccggaa agagcttcgc tgcagaatct gtggtctcag
      781 atgcctcagg cggaacgaga cctgatcgac aatccatcca aagacaacct ggctgcttac
      841 caggatctgg tgcgggcgat tgccagacaa actctggacg aagcaatgaa agtagaaaag
      901 cgaaccagac ctgctacatt ttctcgcgct caaaacgaac tcactaccgt tcgcatcatt
      961 gatgaaaagc tgcatcgcat ggcgctcgtc atgctttcgc cggagaactc tgctttttct
     1021 atgatgaaat ctctcgcgga gattcgcggc ttcctgatgg acatgcgccg atagtgccgg
     1081 gttaacaaat tcctttttcg atatcgcacg cttcctgcgt gggagcgagt acaggctttc
     1141 gcgcagtcaa aactgtctcc accctctctc gcacatcttc atatggaaga tatcctctgc
     1201 aaatgatctg ccctcttccc tccccgatat agatgagagt tggaaaaccg cgaacgcccg
     1261 cagacgctgc gaattcaaaa tccttccagg tctccgcttt catgtcttca ctgttccaga
     1321 gcgcagtaaa ttcctcaggc gaaactccga tctccgcagc cagagggatg taggtctcaa
     1381 gcatggtggt atctttgcta tctctgtaaa aggctgtttg cacctgtgcg aaaaagtcga
     1441 tcacagcgtc tggcttcagc tttcgcacaa tcgccaccgc gcgacaggaa ggttccgtgt
     1501 cgtatacaaa atcgtcgcga tcaaagatct tatacccgaa ctcctgaccc gtagtgcgat
     1561 ggacttcttc ccagtgatgg cggagaaatc gttttaattt ttcgtccatt ttctccgcat
     1621 gagggcccgg gcgaagtccg cccagcacgc aatgaatcgg aagtcttccc tccagatctt
     1681 ctttgagtcg caatagaacg ggagcaaatc cgtaacacca ggagcacatc ggatctccaa
     1741 aatagagcag ctgcgacggt tcgttcataa atacagaatg cctatttttt tccaggaggt
     1801 caatcggatt tggaaagccc gccaggactg cgtcatcaat ctgacaccat ggaaggagaa
     1861 tctttctaca gcctgactcc tgaatccata cttgcatctg tggaagcagc cggccttccc
     1921 gttacaggta gatgtctgca gcgaaactcc ctggagaatc gagtttacga agtagaaatc
     1981 gacattgaaa gcgaaaacaa atcagaacga ttccgaattg cgaagttcta cagaccaggc
     2041 cgctggacaa gagagcaaat cctcgaagag caccaattca tttcagattt acgagcccag
     2101 gaaatcccgt gcgcgccgcc tatagcgctc cccggcggca gcacactggg caaaacagaa
     2161 agtggcatct tcttttctct ttttgaaaga gtgggcggcc gtcagttaga tgaactggga
     2221 ggcacagaaa gagaacaact gggcagactc ctggcaaggg tgcataacgt tggggctgca
     2281 tcaaaatcag ttcacagaat taaactgaac gaagaaacat acggacttca aaacctggac
     2341 tatctacttg aatcggaatc cataccggag cacatggaag aacgttatgc gcaattggtg
     2401 gaagatattt gcgagcacac cgcaaacttg agagaagagc tttccgctcc caatgaattc
     2461 ctgccggcac tagaagaaat caggctgcac ggagactgcc acggcggcaa catactgtgg
     2521 agcaacgccg gaccatttct gattgacttt gatgacatgg tcagtgggcc acctgttcag
     2581 gatctgtggc tgcttgcttc cgattcggaa gaacttgagg ctattgtgaa cggctacgag
     2641 cagatgcgga cattcgatcg tcgcagtctc aagctggtag aacatctccg cgcactccgg
     2701 tacattcatt tcagcgcctg gatcgcccga agatgggaag acccttcttt ccctggagca
     2761 tttccggcat tcggcacgga acaatactgg gccacacaaa tcgacgatct ggtagaacag
     2821 ctagaacgga ttgaaacaac acaatcaccg ttttcggatt gaccaggagt tcccgtcgcg
     2881 cgaagatcac acttcaagat gatcaaaacg cctaacgtct ttaacctcat ctactctgtt
     2941 gtgctgtggg ccatcctgct gacggtaaca ctgttctttg gtatatccgt tatgccattc
     3001 gctcttttcc gcgccagaaa agcagtacac tttggtgcac gcatgtgggg gaagagtctc
     3061 gcttttcttt cacggatgac catccaggtg aaaaacaaag agaaactcta ccgcggcggt
     3121 ccggtcattt acctggccaa tcaccagagc atgttcgaca ttgctgtatt ctactcattc
     3181 ctcgacactg aattc
//
LOCUS       DAORFF010000473         3192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_19776_length_3192_cov_3.328020, whole genome shotgun sequence.
ACCESSION   DAORFF010000473 DAORFF010000000
VERSION     DAORFF010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19776_length_3192_cov_3.328020"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2394)
                     /locus_tag="PKK76_15925"
     CDS             complement(<1..2394)
                     /locus_tag="PKK76_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreAB"
                     /protein_id="HNL70019.1"
                     /translation="MSTETAQDPVISRLYTHLNEEKVTRDLSSVQLQKIKTLDELMQE
                     IQASGKKDELIHTLEENLKDNADSMISRYLLGRIHFHKGDERGTEHLRSLLEDFRTFA
                     RWPIVDHIADGILAHDDSNRVALRAKVESTERLRGRKELKPYLEKLAAIDKKNPDVAR
                     KYAQSILEEDPARALVFLKQAGEAYARTRDYKNLEDVWNQIVEHDPKDLAFFESVERI
                     LVGHREKMRIAAYYSNLVEHYKKIEDWNTVIVLLKKVLEYEPNSRARSDLVRAYRTKY
                     ENHSLLNEFLKMSDLTNHKKPVGPCIASFERNIVFDKGNFVYHRTRGVGKIAEIDKDQ
                     VIIDFKDHPGQKMSIQMAISSLQPLNPEHMWVRAYENPAEMQEIFEQDIPMFLENLLT
                     SFHNKITLPEIKIEVLPFLKGEDWSRWWTRARAEIKKNPRFAFNPRNKNELLLREREI
                     TLADELSLKFQATNDWNDKMAIALETLKDEASSGAAQLCTQYYLDQEESKDRLKRLHS
                     YLFLDLAEKASDDHLGRKISKADMQEFLKAEPVKGLLKISQDTPFPEFKRELIALILK
                     VRPDYAEIVTELFFEVPGNLRTYIMNELARLGQKDAVIRCIDRTLKKTREYPEIFLWI
                     ARAILSGQWTYDWMPWTRQDMLLNVFRFLKPLAAIEKKGTKLKNQALEAVFGTTNITV
                     ESIKKSDLPELLRDAEAGNVRKMAVLFKEISYVPDAQKENFMEFLSSVRPDLNMNAPD
                     KAEEEAEESVSTADLFPAEGIILVSAKGFADRKKHLDNLINVEMPANSRDIGEAQERG
                     "
     gene            2520..>3192
                     /locus_tag="PKK76_15930"
     CDS             2520..>3192
                     /locus_tag="PKK76_15930"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNL70020.1"
                     /translation="MAFPEFSSSRKRASVELEILDFWKQKGVFESQVKATASGPKFVF
                     YEGPPTANGKPGIHHVLSRVFKDIYVRYYGQRGFQIPRQAGWDCHGLPVEREVEKELG
                     ITSKSEIEGKVGLETFNRLCRENVQKYISDWNTFSERLGFWIDLKNAYYTMDNSYIEA
                     VWGLLKNLWDKKLIEKSYKVVPVDPVMGSTLSDAEVAQGYKTVEDPSLTVRFRIADDK
                     YKDTSF"
BASE COUNT          722 a          744 c          807 g          919 t
ORIGIN      
        1 accgcgttcc tgcgcttctc caatgtcgcg agagttagct ggcatctcta cgttgatgag
       61 attgtccaga tgcttctttc tgtcagcgaa gcctttcgcg gagacaagaa tgatcccttc
      121 tgcagggaac aggtctgctg tggagactga ctcttctgct tcttcttccg ctttgtccgg
      181 ggcattcata ttcaggtcag gacgcacgct ggatagaaac tccatgaagt tttctttttg
      241 tgcatccgga acgtatgaga tttctttgaa aagaactgcc atcttgcgaa catttcccgc
      301 ttctgcgtcg cgcagaagtt ctggaagatc gcttttcttg atggattcta cagtgatgtt
      361 ggttgttcca aacactgctt ccagtgcctg gttcttgagt ttggttccct ttttctcgat
      421 ggccgcaaga ggcttcagaa agcgaaacac attgagcaac atgtcctgac gagtccacgg
      481 catccagtca taggtccact ggccggaaag aatcgcgcga gcgatccaga ggaaaatctc
      541 aggatattcg cgggttttct taagggtgcg atcgatgcag cgaatcacag catccttctg
      601 tccgagtctt gccagctcgt tcatgatgta agtgcgaagg ttgcccggaa cttcaaagaa
      661 gagttctgtt acgatctcag cataatcagg acgaactttg aggatcagag caattagttc
      721 gcgtttgaat tctggaaatg gagtgtcctg ggaaatcttg aggaggcctt ttacaggttc
      781 tgccttcagg aattcttgca tgtccgcttt tgaaattttg cgcccgaggt gatcatcgga
      841 tgctttctct gcgaggtcca ggaacagata ggagtgcagt ctcttgagtc tgtctttgct
      901 ttcttcctga tcgaggtagt actgagtgca caattgcgcc gcgccagaac tcgcctcatc
      961 tttgagcgtt tccagagcaa tcgccatctt gtcgttccag tcgttggttg cctggaactt
     1021 cagactcaat tcgtccgcga gagtgatttc gcgttcacgg agtagaagct cgttcttgtt
     1081 acgcgggttg aatgcgaagc gagggttttt cttgatttcc gcgcgtgcgc gtgtccacca
     1141 tctggaccag tcttctccct tgagaaaggg aagaacttct atcttgattt cagggagagt
     1201 gatcttgttg tgaaagctcg taagcaagtt ttcgaggaac atgggaatgt cctgctcgaa
     1261 gatttcctgc atctctgctg gattctcata cgcgcgaacc cacatatgtt ccggattcaa
     1321 aggctgcagg ctggagatgg ccatctggat ggacattttc tggcccggat gatccttgaa
     1381 gtctatgatc acttgatctt tatcaatctc agcgatcttg cctacaccac gagtgcgatg
     1441 gtacacaaag ttgcctttgt caaaaacaat gtttctttca aagctagcaa tgcagggtcc
     1501 caccggcttt ttgtggttcg tgagatctga cattttgaga aattcattca ggagggaatg
     1561 attctcgtat tttgtgcgat acgcgcgaac caggtcggag cgtgctcttg agtttggttc
     1621 gtattccagc actttcttga gcagaacgat tacagtattc cagtcttcga tcttcttgta
     1681 gtgctctacc aggttggagt agtatgcggc gatgcgcatc ttctctctgt ggcctacgag
     1741 gattctctct acgctttcga agaacgcaag atcctttgga tcgtgttcta cgatttgatt
     1801 ccacacatct tccaggtttt tgtagtcgcg ggtgcgagcg tatgcttccc ctgcctgttt
     1861 caaaaacacg agagcgcgcg caggatcttc ttccaggatg gattgggcgt acttgcgtgc
     1921 aacatccgga ttctttttgt caatggccgc tagtttttcc agatatggtt tgagttcttt
     1981 tcttccgcgg agtctttctg tggactccac tttcgcgcgc agtgccacgc ggtttgagtc
     2041 gtcatgggcc agaattccat ccgcaatgtg atctacgata ggccagcgtg caaaggttct
     2101 gaaatcctct agaagagaac gaaggtgttc ggtgccgcgt tcgtctccct tgtgaaaatg
     2161 gattctaccc aggaggtagc gcgaaatcat agagtccgcg ttgtccttca gattttcttc
     2221 cagagtgtgg atcagttcat ccttcttgcc agaggcctgg atttcctgca tcaattcgtc
     2281 tagggtcttg atcttctgaa gctggacgct agaaaggtcg cgggtgacct tctcctcatt
     2341 gaggtgcgtg tataacctgg atatgacggg gtcctgtgct gtttctgtgc tcatccgatg
     2401 ctgctttgtt ctataaaatg gagttagaat ccaacatccc cggatttacc ctccaggtca
     2461 aaccctttcg ctaactacga tatgtttgaa aaaagacccc gccgggccaa atctggcgaa
     2521 tggcgttccc agagttctca tccagcagaa agcgtgcttc cgttgagctt gagattctgg
     2581 atttttggaa gcaaaagggt gtttttgagt ctcaggtcaa agcaactgca tccggaccca
     2641 aattcgtttt ctacgaaggt ccgcccacgg caaacggcaa gccaggaatt caccatgtac
     2701 tgagtcgagt cttcaaagat atttacgttc gctattatgg acagagaggt tttcaaatcc
     2761 cgcgtcaggc aggttgggat tgccacggtc ttcccgtgga gagagaagta gaaaaagagt
     2821 tagggatcac ttctaaatct gaaatcgaag ggaaggttgg actcgaaacc ttcaatcgtc
     2881 tttgccgcga gaacgttcag aagtacatct cagactggaa cacgtttagc gagagactcg
     2941 gcttctggat cgatttgaaa aatgcatact acacgatgga caattcgtac atcgaagctg
     3001 tctggggctt gctcaagaac ctgtgggata agaaactcat cgaaaaaagc tacaaagtag
     3061 ttcctgtcga tcctgtgatg ggttccactc tttcagacgc agaggtggca cagggttaca
     3121 aaactgtaga agatccgtct cttacagttc gctttcgcat agcagatgat aaatacaaag
     3181 atacttcttt tc
//
LOCUS       DAORFF010000474         3190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_19801_length_3190_cov_3.329825, whole genome shotgun sequence.
ACCESSION   DAORFF010000474 DAORFF010000000
VERSION     DAORFF010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19801_length_3190_cov_3.329825"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..807
                     /locus_tag="PKK76_15935"
     CDS             187..807
                     /locus_tag="PKK76_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70021.1"
                     /translation="MIKPEKAAWELKNEKNKAGVRWLLVVLIGGYLGSLLQNGGVLPG
                     ADRLFSWYYIGAVTAFVAILNLAVTLYILRAKDQGTFHPAVKYLTMVTDLLAVSLVLL
                     PTGGSESMFFLVYFVIIVSNALRYGMRLAVLGVLVFNILYVLVLAYQYAPNFDIPGFQ
                     KEVLKVSGVWLIGLYTGYLARRFEMLQGEVEKYQKLLANLMAEKKQ"
     gene            804..1184
                     /locus_tag="PKK76_15940"
     CDS             804..1184
                     /locus_tag="PKK76_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70022.1"
                     /translation="MTEKNPQHENAELEASVQKAFRRGNINFDFLQPDSTPREIAEEE
                     RSERRTNSRQGKRGLFNRGRPSRPISRAQVYVWAALFGVSVTLVLVLFFSGKSILLLL
                     GLPILFGTTLWSVIMLTLFLARPR"
     gene            complement(1150..1896)
                     /locus_tag="PKK76_15945"
     CDS             complement(1150..1896)
                     /locus_tag="PKK76_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70023.1"
                     /translation="MSLPSHPVRRALYRLVTSLFASVSTILLSVYQCTLEFEVEGEDH
                     LRLLKAKGENYVVAVWHTFVDAAVFCLHHRNLVIYSDHPRTPEYENSWTHFFREIGLK
                     TIRALGFEVLDASLGKQSAGIINFIKKIQSGSPALVAPDGPHGPIYESKPGVLYMAHK
                     ASSVVVPVGVGFSRRIVGPNWDDFNLPLPFSRVALVIGEPIYPSDASEEALKTKAREL
                     EETMDRLCFRANEILFGPASSEAAQEKVSA"
     gene            complement(1912..3060)
                     /locus_tag="PKK76_15950"
     CDS             complement(1912..3060)
                     /locus_tag="PKK76_15950"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNL70024.1"
                     /translation="MRTTNSFYTGQLIGSVLSEEDNDLSSNGTKVGPSNSSGFTFTCD
                     STTTVCTVKARTVREQGDSYLAGFYNTFTAGELFQFEAYYLGVYKKWIQKTSPLALPD
                     AELMSQDRSRQRDNLHTIGARLTNRMGTDRKAQWEIPIDWSIEYAWQSGPTGRRIDAS
                     WDYLRVTVPSPAGPVRAYTEREKYNAYAFAADAGYTIASFLRIGVAYNIASGDKNRSD
                     GVVTTFNSLFPTSHGLYGQADLVSWQNMIGKSIQMNLMFGKFGKLKLAAWQIDKMRLE
                     DGWYDGTGTLKTNESTESFSNTRFSPLQDSSGYRASAYLRKGLLKEYDLTYIVPWKRV
                     EIGLGYSRIQAGDSIREAKNDTLNTTISRRPRFDPRADFAYLMLTMSF"
BASE COUNT          805 a          781 c          774 g          830 t
ORIGIN      
        1 gggctatacc ggaaaattcg tagaaggaat cgcactgggc gttttatcca aagaactggg
       61 atggccaggc gcgctcagtt tcatcgcagc gtcctgcatc gttgcgattc tgatgcttgc
      121 gtttacctgg aaattgaaac cggcagaata gttgacacga tctgcggcga agagtgactc
      181 aaacagatga tcaagccaga aaaagcagcc tgggaactaa aaaacgaaaa gaacaaggca
      241 ggtgtacgct ggctgctcgt agttctcatt ggcggctatc ttggatctct tctccaaaac
      301 ggaggagtgc ttccaggtgc tgacagactc ttttcctggt attacattgg ggcggtcact
      361 gctttcgtcg ccattctcaa tctggctgtg actctctaca ttcttcgcgc gaaggatcag
      421 ggcacatttc atcctgcagt gaagtatctt accatggtca cggatctctt ggcagtctct
      481 ctggtccttc ttcccacagg cggaagcgaa agcatgttct ttcttgtata tttcgtaatc
      541 atagtttcaa acgcactgcg atacgggatg aggctcgccg tcctgggagt gctggtgttc
      601 aatattttgt atgttctggt gctcgcttat caatacgctc ccaatttcga cattccagga
      661 tttcaaaaag aagttctgaa ggtttcaggt gtatggctca ttggactcta cacgggctac
      721 ctggcgcgaa gattcgaaat gctgcaggga gaagtggaaa aataccagaa gctcctggcc
      781 aacctgatgg ctgagaaaaa acaatgaccg aaaaaaatcc acagcacgaa aacgcggaac
      841 tggaagccag cgttcagaag gcattccgac gcggaaacat caactttgat ttcttacaac
      901 cggactctac tccacgcgaa atcgcagaag aagaaagatc agaaagacgc accaactctc
      961 gccagggaaa acgcggcctc ttcaaccgcg gccgtccatc cagacccatt tcgcgcgcac
     1021 aggtttatgt atgggctgct ctttttggag tcagcgtcac tctggttctg gttcttttct
     1081 tttccggcaa aagcattctg ctgttgcttg gacttcccat tctattcggg actacactgt
     1141 ggtccgtaat tatgctgaca ctttttcttg cgcggcctcg ctagaggccg gtccaaacaa
     1201 gatttcattt gcgcgaaagc aaagtctatc catggtttct tctagttcgc gcgcttttgt
     1261 tttgagcgct tcctccgaag catccgatgg gtagatgggt tcaccaatca caagtgcgac
     1321 gcgcgaaaac ggaagaggca aattgaagtc atcccagtta ggaccaacaa tccgcctgga
     1381 aaaaccaacg cctaccggaa ccacaacgga actggctttg tgcgccatat agagcacacc
     1441 gggcttagat tcgtagatgg gcccgtgagg accgtccgga gctacgagag cgggagatcc
     1501 gctctgtatt ttcttaataa agtttatgat tcccgctgat tgctttccca gagaggcgtc
     1561 cagcacctcg aatccaagag cgcggattgt ctttaaacca atctcgcgaa aaaaatgagt
     1621 ccagctattc tcatactcgg gagtgcgcgg atgatctgaa tagatcacga ggtttcgatg
     1681 gtggaggcag aagaccgccg catccacgaa tgtgtgccag actgccacca cgtagttttc
     1741 tcccttcgcc ttcaaaaggc gaaggtgatc ttctccctct acttcaaact cgagtgtgca
     1801 ctgatagaca ctgagaagga tcgtgcttac agaagcaaac agactggtga ccaggcgata
     1861 gagagctcga cgcactggat gagatggcag agacatgctt cctccggact gctagaaact
     1921 catggtaagc atcagatacg cgaaatctgc gcgcggatca aatctaggcc tgcgcgaaat
     1981 ggtagtgttc agcgtatcgt tctttgcttc gcgaatcgag tctccggcct gaattcggct
     2041 gtagcccaat ccaatttcaa ctcttttcca gggaacaata tacgtaaggt cgtattcttt
     2101 tagcaaaccc tttcgcagat acgcggatgc acgatatccc gatgagtctt gcagaggcga
     2161 aaagcgcgtg ttcgaaaagg attccgtgga ctcgtttgtt ttcagtgtgc ctgtgccatc
     2221 ataccagccg tcttcgagac gcatcttgtc tatttgccag gccgcgagtt tcaactttcc
     2281 aaacttacca aacatgagat tcatctgaat cgatttgcca atcatattct gccagcttac
     2341 cagatctgcc tggccataga gaccatgact cgttggaaaa aggctgttga aagtagtgac
     2401 cactccatcg gatcggttct tatcaccgct ggcgatgtta taagcaactc cgattcgcag
     2461 aaaggacgcg atcgtgtatc cagcatccgc ggcgaatgca tatgcattgt atttctctcg
     2521 ttcagtgtag gcgcgcacgg ggcctgcagg ggaagggact gtgactctca agtaatccca
     2581 gcttgcatct atgcgccttc cggtgggccc ggattgccag gcatattcta tggaccagtc
     2641 gattggaatt tcccattggg cctttctatc ggtgcccatg cggttggtga gtcttgcacc
     2701 aatcgtgtga aggttgtctc gctgcctgct tctatcctgg gacatcaact cagcgtccgg
     2761 tagtgccaga gggctggttt tttgaatcca cttcttgtag actcccagat aatacgcctc
     2821 gaactgaaaa agttccccgg ccgtaaatgt attgtagaag ccggcaagat acgaatcccc
     2881 ctgctctctg accgtgcgcg ctttgactgt gcacacggtc gtagttgaat cgcaagtaaa
     2941 tgtaaagccg gatgaattcg aaggaccaac ctttgtgccg ttagacgaca aatcgttgtc
     3001 ttcttctgaa agaacagatc cgatcagctg accggtgtag aaactgtttg tggttctcat
     3061 aaatcaaaag aaaaatctga attttcttgt gattgtatag ttttttcttc actattaact
     3121 tctggaatat caaaagaaaa atcattttct ttataatttc taatataaaa atattttata
     3181 ataatggata
//
LOCUS       DAORFF010000475         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_19874_length_3183_cov_3.235934, whole genome shotgun sequence.
ACCESSION   DAORFF010000475 DAORFF010000000
VERSION     DAORFF010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19874_length_3183_cov_3.235934"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3149)
                     /gene="gltB"
                     /locus_tag="PKK76_15955"
     CDS             complement(<1..3149)
                     /gene="gltB"
                     /locus_tag="PKK76_15955"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNL70025.1"
                     /translation="MAIVENTSRAQGLYDPGLDKDSCGVGFVVDIQGRKTRRIVDLGL
                     QVIHNLEHRGAVGADPLTGDGAGILLQMPDKFCRRAAKEVGIDLPPEGRYAVGFMYLP
                     QNRTQRKAVETIIEKVTVDEGQEFLGWRDVPVQPEFTGEGARSTLPQFRQCFIAASDD
                     IKDTLEFDRRLYLIRRVIDRRVRAEHKLDRSRYYVASFSSRTIIYKGMLLADQLSRFY
                     VDLQAPDMESALALIHMRFSTNTFPTWDLAHPFRMIAHNGEINALRGNINWMAARQMM
                     MESPLYGKDLKRMLPIIMEGQSDSATFDTVLELLVMAGRSMPHAMMMMVPEAWSKNPD
                     MEESRRAFYEYHATMMEPWDGPAAIAFTDGTQIGATLDRNGLRPARYIVTKDDLVILA
                     SEAGTLPIEASNIARSGKLQPGKMFLIDLNEKRIIEDEEIKRQVMTKQPYGKWVEQNM
                     IRMNELPDPTFVHQPDHLTILERQRVFGYTSEDINATIRPMSVTGEEAVGSMGVDAAL
                     AVLSDRPQPLFRYFKQLFAQVTNPPIDPIREELVMELTAYIGPEGNLLDETPAHAHRL
                     ELEHPVLTNNDLEKIRTIARGHFQAVTLNILFDPEAKSGMRNRLDQVCDEAANAVRSG
                     VNLIILSDRGVGPNVAPIPSLLAVAAVHHFLIRAGLRTKTGIVLESGEPREVSHFALL
                     LGYGANAINPYLAFETLADLIEEGGMPEIKSFKDARKNYIKAIGKGLFKIFSKMGIST
                     LQSYCGAQIFEAVGLDSELINAYFTGTPSRVEGCSAEMIEEEVLRRHAQAYDATFHPG
                     LLDPGGVHYWRKEGEAHLYSPTTIHKLQLATETGDYKVFKEFSKAIDDQSKHRITLRS
                     LFDFDTNAQPVPIEEVEPVSTIVKRFATGAMSFGSISWEAHTTLAIAMNRLGGKSNTG
                     EGGEDSIRYAKLENGDSMRSAIKQVASARFGVTTNYLVNADELQIKMAQGAKPGEGGQ
                     LPGHKVDKHIGRLRFSTPGVTLISPPPHHDIYSIEDLKQLIFDLKNVNPKARISVKLV
                     SEVGVGTVAAGVAKCHADH"
BASE COUNT          756 a          843 c          769 g          815 t
ORIGIN      
        1 atatgatctg catggcattt tgccacgccg gcagccacag taccaacgcc aacctcgctc
       61 accagcttca cgctgatgcg cgctttcggg ttcacatttt tcaaatcgaa gatcagctgt
      121 ttcaaatctt caatcgaata aatatcgtgg tgcggtggtg ggctgatcaa ggtcacacct
      181 ggcgtactga aacgcagccg accaatgtgt ttgtccactt tgtggcccgg cagctgtccg
      241 ccttctcctg gtttcgcgcc ctgtgccatc ttgatctgca gttcgtctgc attgaccaga
      301 taatttgtcg taacgccaaa ccgagcagat gcgacttgct tgattgcgct tcgcatgcta
      361 tcgccatttt caagcttcgc gtagcggatg gagtcttccc caccctcacc tgtattgctt
      421 tttccgccca ggcgattcat cgcaatggca agagttgtat gcgcttccca ggaaatagac
      481 ccgaagctca tcgcacctgt tgcaaatcgc ttcacgatcg tacttaccgg ctccacctct
      541 tcgatgggca ctggctgagc gttcgtgtca aagtcaaaaa ggctacgcag tgttatgcga
      601 tgcttggact gatcgtcaat tgcctttgaa aactctttga atactttgta gtcgcctgtt
      661 tcagtagcaa gctggagctt gtggatggtc gtgggagaat atagatgagc ttccccttct
      721 tttctccagt agtgaactcc gccaggatct aacaagccag gatggaaggt tgcatcatat
      781 gcctgagcgt gccgacgaag tacttcttcc tcgatcattt cagcgctaca accctctacg
      841 cgacttggcg tgcctgtgaa gtatgcgttg atcagctctg aatccagacc caccgcttca
      901 aaaatttgcg cgccgcaata ggactgcaga gtagaaatcc ccattttgct aaagatttta
      961 aacaatccct tgccaatggc tttgatgtaa ttcttgcgag cgtctttgaa gctcttgatt
     1021 tcaggcattc cgccttcttc gatcaagtct gcaagagtct cgaaagcaag ataagggttg
     1081 atcgcgttgg cgccgtaacc cagaagcagc gcgaaatgag agacttctcg tggctcccct
     1141 gactcaagta caatgccagt cttcgtgcga agaccggcgc gaatcaagaa atggtgaacc
     1201 gcggccactg caagcagact ggggatagga gccacgttcg gtccaactcc cctgtcagaa
     1261 aggatgatca ggtttacacc gctgcgaacc gcattggctg cttcatcgca cacttgatcc
     1321 agccgattgc gcatgccgct ttttgcttct ggatcaaaga gaatgttcag agtcactgcc
     1381 tggaaatgac cgcgtgcaat cgtgcgaatc ttttctagat cattgttcgt gagaacaggg
     1441 tgttcgagtt cgagacgatg cgcatgcgca ggcgtttcat cgagaaggtt tccttccggg
     1501 ccaatgtatg ccgttagctc catcacaagc tcttcgcgaa tcggatcgat cggagggtta
     1561 gtaacctgtg cgaaaagctg tttgaaatag cggaacagag gctgcggcct atcagacaga
     1621 acagcaagag ctgcgtccac acccatggac ccgactgcct cttcgcccgt gacagacata
     1681 ggtcgaatgg tggcattgat atcttctgat gtgtaaccaa acacgcgctg tctttcaaga
     1741 atggtaagat ggtccggctg gtgaacgaac gttggatccg gcagttcgtt catgcgaatc
     1801 atgttctgct caacccattt gccgtaaggt tgctttgtca tgacctgacg tttgatttct
     1861 tcatcttcga tgattctttt ctcattcaga tcaatgagaa acattttgcc cggctggagt
     1921 tttccactgc gcgcgatgtt cgacgcttca atcggtagag ttcccgcctc agacgcaagg
     1981 atcacaagat catcttttgt aacgatgtag cgcgcaggac gcagcccgtt tctgtccaga
     2041 gtcgcaccaa tttgagttcc atctgtaaat gcgatagccg ctggaccatc ccacggttcc
     2101 atcatggtag catgatactc gtagaatgcg cgacgactct cttccatgtc cgggttcttt
     2161 gaccaggctt ctggaaccat catcatcatc gcatgaggca tgcttctgcc tgccatcacg
     2221 agaagttcaa gaaccgtatc aaaagtggca gaatcactct gcccttccat gatgatcgga
     2281 agcattctct tgagatcttt gccgtataac ggtgactcca tcatcatttg acgggcagcc
     2341 atccagttga tgtttccgcg aagcgcattg atctcaccgt tatgcgcaat cattctgaat
     2401 ggatgagcaa gatcccaggt aggaaacgta ttcgtagaaa agcgcatgtg gataagcgcc
     2461 agagcagatt ccatatccgg agcctgcaga tccacgtaaa acctggagag ctgatccgcc
     2521 agaagcatgc ccttgtaaat aatggtgcga cttgaaaaag aagcaacgta gtaacgactg
     2581 cgatccagtt tgtgttctgc gcgaacacga cgatcgatga cgcgtcgaat cagatacaat
     2641 ctgcgatcga actcaagagt gtccttgata tcgtctgatg ctgcaatgaa acactgacga
     2701 aattgaggaa gagtagagcg agccccttct cccgtaaatt caggttgaac aggaacgtct
     2761 ctccagccca aaaattcctg gccttcatcc accgtaactt tctctatgat cgtctcaaca
     2821 gcctttcgct gtgtacgatt ttgaggcagg tacatgaagc ctacggcata gcgcccttct
     2881 ggtggcagat caattccaac ttcctttgca gcgcgtctgc aaaacttgtc tggcatttgt
     2941 aaaagaattc ccgcgccgtc ccctgtgaga ggatctgcac ccacggcacc gcgatgctct
     3001 agattgtgaa tgacctggag cccaagatcc acgattctac gcgtttttcg gccctgaatg
     3061 tcgacaacga agccaacgcc gcaagagtct ttgtccaggc ctggatcata aagaccctgt
     3121 gcccgcgatg tgttttctac tatagccata tcctgagccc cgaattgcca aaaaactgcg
     3181 ggt
//
LOCUS       DAORFF010000476         3151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_20196_length_3151_cov_3.439922, whole genome shotgun sequence.
ACCESSION   DAORFF010000476 DAORFF010000000
VERSION     DAORFF010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20196_length_3151_cov_3.439922"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1239
                     /locus_tag="PKK76_15960"
     CDS             94..1239
                     /locus_tag="PKK76_15960"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNL70026.1"
                     /translation="MSRLHGVLVFAAIVAFSAACLKKASTAVPVETAAISLLVVGDVT
                     ADGQPVKTGQLLKGSPWIIVGPASIMDLQVRDSEQGVVFRLKENSKFRLSSLDRGNGK
                     TFVAQLQSGQLLASVKKRSADEEVRINTPTALAGVRGTKFDVYVFQDGSIRTSVYEGS
                     VVTRARLNGVDDVPAELVENSEALKKIIKQIEKSEKVLEPGDQATVAHFVVDAEVQNA
                     IQTAITDPEVRALAEKKDRTPEDIKATAAALDKKLSPQLAVLEKKIQEDLPAAKVEVK
                     ERPPAPPGGYPVPDEFKEMQPVDTSRSDTEEKTRKEVEKRNQDENIRKNLMVRIEKVT
                     GGKLDQLILKDGKTISGVIRQVGEDYIVTTPEGNQSISGSQVGEMRF"
     gene            complement(1273..>3151)
                     /locus_tag="PKK76_15965"
     CDS             complement(1273..>3151)
                     /locus_tag="PKK76_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70027.1"
                     /translation="RGDLKVKQKPFKDMPVAKTEYSSRKGFRATWIVAGCADQPGWVY
                     SRIEYDAPGPCEIVIGIRPFNPEGAALITDLQLHMEPQGAVLAINKNRDIFLRKSPDE
                     VRFSNLKTGDAYFESEPHGIVHCPHGIATGCIIYRVSGHGTIHFYARNDRTITEDIPL
                     SFLANETEVHQSLASWERQIERGAKFISARGSWNKAARIFSGYVLSLQTGREITPGVF
                     TYNQFWFRDAAYMVSSLTAWGFIAEAKAVLESYAERQSRDGFFKSHEGEWDSNGQAIW
                     TLSHFIEHTGDLELLKKVYPAIRRGAQWIIKKRRQGPRRLILPAGFSAEHLGPADHYY
                     WDNIWSIAGLLHAAHLAGLMGETKDENYFTRECDEYRRDLLEISSQERQKYGLITAAP
                     DRPIDSGMIGNICAVYPLDLVLFPEDEMRRTVRQIHRDFFTHDLFFHPIIHSGYNIYL
                     SLQMAQSHLRLGDVRQARRIFKRVLKSKSELWTYPEAIHPNTGGGVMGDGFHGWAFAE
                     VLLLLREFVLHAQNGVLSIFRGLRNRELMGEFFFGPFPVSAGRVSISGSMSNKRGRIQ
                     IDFDRASTMRSYRIHVPGIRSRIADIKGSGCASFHFDRKKAIIEITGPTSEVEIELIL
                     S"
BASE COUNT          781 a          743 c          846 g          781 t
ORIGIN      
        1 catgtcaaat gttttctggt tgaccaggcc ccgaagcagg ttcacctttg ggcgtctggc
       61 gggcgtcgcg tcgggggaag gtaggtttcg catatgtcta ggctgcatgg tgtactcgtt
      121 tttgccgcaa tcgtcgcttt ttctgcggcc tgtttgaaga aagcttcgac tgcggtgccc
      181 gtagaaacgg ccgccatttc cctgcttgtc gtaggagacg taaccgcgga cggacaaccg
      241 gtgaaaaccg ggcagcttct gaaaggcagc ccgtggatca tcgtgggtcc ggcctccatc
      301 atggatctgc aggttcggga ttcggaacag ggcgttgtct ttcgcctgaa agaaaatagc
      361 aaatttcgtc tgagttcact cgaccgtgga aatggaaaga cgtttgtagc gcagctccag
      421 tcaggtcagc tacttgcctc tgtgaagaag agaagcgcag acgaagaggt tcgcatcaac
      481 acgccaactg ctctggcggg agttcgtgga accaagtttg atgtatacgt gtttcaggat
      541 ggatccattc gcacaagtgt ctacgaaggg agtgtagtga ctcgtgctcg cctcaatggc
      601 gtggatgatg ttcctgcgga actcgttgaa aatagcgaag cactcaagaa gatcatcaag
      661 caaatcgaaa aaagtgaaaa agttctcgaa ccaggagacc aggcgactgt cgcacatttc
      721 gtagtggatg cggaagttca aaacgcaatc cagaccgcca taacagatcc ggaagtgcgt
      781 gctcttgcag agaagaaaga tcgcactcca gaagatatca aagcgactgc ggccgccctc
      841 gataagaagc tttctcctca gctcgcagtt ttggagaaga agattcagga agatcttcct
      901 gcggcaaagg tagaagtgaa ggaaagacca ccggctccgc cgggcggata tccggtgcca
      961 gacgaattca aagaaatgca gcctgtagat acttcgcgtt cagatacaga agagaagacg
     1021 cgcaaagaag tagagaagcg caatcaggat gaaaacattc gcaagaatct gatggtgcga
     1081 atcgagaaag ttacaggcgg gaagctcgat cagctcatac tcaaagacgg aaaaacaatc
     1141 agcggtgtga ttcgtcaggt aggggaagat tacatcgtga ctacgccgga aggaaatcag
     1201 tctatttcgg gaagtcaggt tggagagatg agattctgat ttagattgga ccggcgggca
     1261 agtagcagcc ggttaagaaa ggatcagctc gatttctact tcgctcgttg ggcctgtgat
     1321 ttcgatgatt gctttttttc tgtcaaagtg aaaggaagcg cagccgcttc ctttgatgtc
     1381 tgcgattctg ctgcgaatcc ctggaacgtg aattctgtac gatctcatgg tactggcccg
     1441 gtcaaaatcg atttgaattc tgccgcgctt gttgctcatg cttccggaaa tgctgactct
     1501 tccagcggaa actggaaatg gaccaaagaa gaattcaccc atcagttcgc gatttcgcag
     1561 gccgcggaaa atagaaagca caccgttttg agcgtgcaaa acaaattcgc gcagcaagag
     1621 aagcacttct gcgaaagccc agccgtgaaa tccatctccc ataactccgc cgccggtatt
     1681 cggatgaatt gcctcggggt aagtccatag ttcgctcttg ctcttgagta ctctcttaaa
     1741 gatccttcgc gcctgcctca catctcccag gcgaaggtgc gattgcgcca tttgaaggct
     1801 gaggtagatg ttgtagcccg aatgaatgat tggatgaaag aatagatcgt gtgtgaagaa
     1861 atcgcgatgg atctgtctga ctgtgcgtcg catttcgtct tccggaaaaa gaaccagatc
     1921 cagcggataa acggcacaga tattcccaat cattcctgaa tcaatgggac gatcaggcgc
     1981 agccgtgatc aggccatact tctgtctttc ctgggaagag atttctagca gatcccgcct
     2041 gtattcatcg cactcgcgcg tgaagtagtt ttcgtctttt gtttctccca tcagtccggc
     2101 aagatgcgcg gcgtgaagaa gccctgcaat agaccagatg ttatcccagt agtaatgatc
     2161 cgccgggcca agatgctccg cgctgaatcc ggcaggaagg atcaatcggc gcgggccctg
     2221 tctgcgtttt ttgataatcc attgcgctcc ccgacgaatg gcagggtaaa ctttcttaag
     2281 taattcgagg tctccggtgt gttcaatgaa gtgcgacaga gtccagatgg cctgcccgtt
     2341 ggaatcccat tctccttcgt gcgatttaaa gaaaccatcg cgtgactggc gctctgcata
     2401 gctttcgagt actgcttttg cttctgcaat gaatccccag gcagtcaggg aagacaccat
     2461 gtatgctgcg tcgcgaaacc agaattgatt gtaagtgaat acacctggag tgatttcgcg
     2521 tccggtttgt aaggagagaa cgtagccgga aaatattctt gctgctttgt tccagcttcc
     2581 tcttgcagaa atgaatttgg ccccgcgttc gatttgtctt tcccagcttg ccagggattg
     2641 atgaacttct gtttcgtttg cgagaaagga gagaggaata tcttccgtga tggttctatc
     2701 atttcttgcg tagaaatgaa tggttccgtg accgcttact cgatagatga tgcagccagt
     2761 ggcaattccg tgcgggcagt gcacgattcc gtgcggttcg gattcaaagt aggcgtctcc
     2821 ggttttcaaa ttcgaaaacc gaacttcatc cgggctcttt ctcaaaaaga tatcgcggtt
     2881 cttgttgatg gcaagtaccg ccccttgcgg ttccatatgg agctgaagat ctgtgatgag
     2941 tgctgctcct tccggattga aaggacgaat tccaattacg atttcgcatg ggcccggagc
     3001 atcatattct atacgagaat atacccagcc tggttgatct gcgcagccag ctacaatcca
     3061 tgtggcgcga aaccccttgc gtgaggaata ctctgttttc gcaacaggca tgtccttgaa
     3121 aggcttttgt tttactttga ggtctcctct g
//
LOCUS       DAORFF010000477         3149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_20224_length_3149_cov_3.430834, whole genome shotgun sequence.
ACCESSION   DAORFF010000477 DAORFF010000000
VERSION     DAORFF010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20224_length_3149_cov_3.430834"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="PKK76_15970"
     CDS             <1..1179
                     /locus_tag="PKK76_15970"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNL70028.1"
                     /translation="KKCFALRIASTMARDQGWLAEHMLILGVQDPQGKKTYICAAFPS
                     QCGKTNFAMLIPPKELQNEGWKVTTVGDDIAWIKPDKQGHLHAINPEAGYFGVAPGTN
                     WETNPNAMATIKGNTIFTNVALTKDGDVWWEGLSDEVPDGMTDWNGKPYDKNSGKPAA
                     HPNARFTAPASQCPSVDPSWEDPKGVPVAAFIFGGRRTTTVPLVYQAFNWNFGVYLAA
                     TMGSETTAAATGQVGVVRRDPFAMLPFMGYHAADYFTHWLNIGRTLPAAPRIFGVNWF
                     RRDENKKFLWPGYGENMRVLKWIVERVQGKVSAVESPIGWVPRHEDLDWKGLSFSKDD
                     FNRVMTLDREMWKNEVASHEDLFMKLYDRLPKEFIWMRELLMSSLWRSPETWKASDPT
                     "
     gene            complement(1192..2469)
                     /locus_tag="PKK76_15975"
     CDS             complement(1192..2469)
                     /locus_tag="PKK76_15975"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL70029.1"
                     /translation="MLQRLVVVLTIFLAIFADGCSRYAQDCAIMDARCNAAIFALAFR
                     RLTESRFAYVAVTAGTIYEYSVSRTNGTLTQIGTVPGAGAGATALTMDPQSRFLFAVS
                     ATATNVSSYTINSRTGALSLVGTYTVGAGAVQAAVDPLGRYLYVSDSTTTTVHMFSID
                     QTTGALTGIAGGTFTVLTGPQIPAFDSFGRYAYISTNSVPRVNLLNILSDGSLSNIAA
                     AITPGASPLQGSIDPLNHALYVPNNGAGTISMYTIGSDGSLNSLGTVSPGGAPSTLTI
                     DPTGRFLYTRTGTANIWMYSIDQTTYALAPIGGGTIASGTNPRMLSIHPSGRFAYAPD
                     QSTATLYMYSIDQTTGALTALSGTSRATGGTATAGCAFDAPGSYLYQADGGGSTISQY
                     YVDSSSGQLWSQGVLTAAATPRGPLAMANYNTY"
     gene            2606..>3149
                     /locus_tag="PKK76_15980"
     CDS             2606..>3149
                     /locus_tag="PKK76_15980"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNL70030.1"
                     /translation="MPGVRLKKDQMAWICDPAWIGFDTSDQIPDGPGTFGQNRARDAI
                     EFAANMGRPGYNTAVVGEPGSGRHFLARRLLEKRAATEAPPDDWCYVNNFEDSTRPRV
                     LMLPAGRGAGLKADMHRFAAELGSTMSSAFEAEEYRRHIEQIQNELKAREETALRELG
                     ASSLKEGIALISTAQGFVFTA"
BASE COUNT          789 a          780 c          867 g          713 t
ORIGIN      
        1 aaaaaatgtt ttgctcttcg cattgcatcc acgatggcgc gagatcaggg ctggcttgcg
       61 gagcacatgc tcatccttgg tgtgcaggat ccacaaggaa agaagaccta catctgcgct
      121 gcatttcctt ctcagtgtgg aaaaacgaat ttcgccatgc tgattccacc aaaagaactg
      181 caaaacgaag gttggaaagt cactacagtg ggagatgaca ttgcgtggat caaaccggac
      241 aagcaaggtc atctacatgc aatcaatccg gaagcgggtt acttcggcgt tgctcccggc
      301 acaaactggg aaaccaatcc gaatgcaatg gcaaccatca aaggaaatac aattttcaca
      361 aacgttgcac tcaccaaaga cggagacgta tggtgggaag gcctgagcga cgaggttcca
      421 gacggaatga cagactggaa cggaaagcca tacgacaaga atagcggcaa acccgcggcg
      481 catccaaacg ctcgcttcac ggctcccgct tctcaatgcc catctgtaga tccatcgtgg
      541 gaagatccta agggagttcc agtggcagcg ttcatcttcg gaggacgtcg cactacaact
      601 gtgcctctcg tataccaggc attcaactgg aacttcggag tgtatctggc agccaccatg
      661 ggctcagaaa caacagcggc ggcgacaggc caggtgggag tcgtcagacg tgatccgttt
      721 gctatgcttc cattcatggg ctatcatgcg gcggattact tcacgcactg gttgaacatt
      781 ggaagaacgc ttcctgctgc tccaagaatc tttggagtca actggtttcg tcgagatgag
      841 aataagaaat tcctgtggcc tggctacggc gaaaacatgc gcgttctcaa atggattgtg
      901 gaacgagtgc aaggaaaagt ctccgcagtg gaaagtccca ttggatgggt tccgcgtcat
      961 gaagacctgg actggaaggg actcagtttc tccaaagatg acttcaaccg cgtgatgact
     1021 ctggatcgtg aaatgtggaa gaacgaagtg gcttcgcatg aagatctttt catgaagctc
     1081 tacgacaggc tgccaaaaga atttatctgg atgcgagagc tcctgatgtc ttctctctgg
     1141 cgttcgccgg aaacctggaa agcaagcgat cctacctgat cgcctgccac gtcaataggt
     1201 gttgtagttt gccattgcca gcggccctct cggagtggcc gcggcagtca acactccctg
     1261 ggaccatagt tgtccgctcg atgaatctac gtagtactga gaaatagtag atccccctcc
     1321 atccgcctga tagagatagc tgccgggcgc atcaaatgcg catccagctg tcgctgttcc
     1381 gccagtcgcc cgggatgtgc ctgaaagtgc agtgagtgct cctgtagtct ggtcgatcga
     1441 atacatatac agcgtggccg tggattgatc aggagcgtat gcaaatcttc ccgatgggtg
     1501 aatgctgagc atgcgcggat tggtgccgga tgcaatggtt ccacctccga tcggtgcgag
     1561 cgcgtatgtg gtctggtcaa tcgaatacat ccagatgttg gcagttccgg ttcgcgtata
     1621 aagaaatctc cctgtcggat cgatcgtaag agtggaaggc gcgccaccag gagatacggt
     1681 tccaagagaa ttcaagcttc cgtctgatcc tatcgtatac atagagatgg tgcctgcgcc
     1741 attgtttgga acatagagtg catgattgag tgggtctatg cttccctgaa gtgggcttgc
     1801 tcctggcgta atggccgctg cgatgttgct caagcttccg tctgaaagta tgttcagtag
     1861 attcactctg ggcacactgt ttgtagaaat gtatgcgtat cgcccgaaag aatcaaacgc
     1921 tggtatctga ggaccagtga gtacagtgaa cgttccgcct gctatgccag tcagagcgcc
     1981 tgtagtctga tcaatcgaaa acatatgaac ggtggtggtt gtgctatcgc ttacatacag
     2041 gtagcgaccg agtggatcca cggcagcctg cacagcgccg gcgccaacgg tgtaggttcc
     2101 gaccaatgaa agtgcacccg ttcgactgtt gattgtatac gaagatacgt ttgttgcagt
     2161 cgcagaaaca gcaaaaagaa aacgagactg cggatccatg gtcagggccg tggcgccagc
     2221 gcctgccccg ggcactgtgc cgatctgcgt gagcgtgcca ttggttcttg atacagaata
     2281 ctcgtatatg gttccggctg tcacggccac atacgcaaaa cgggattcag tgagacgtct
     2341 aaaagcaagc gcgaagatcg cagcattgca tctcgcatcc atgatcgcgc aatcctgagc
     2401 gtagcggctg cagccatctg caaaaattgc cagaaagata gttagaacaa caaccaatcg
     2461 ttgcaacata tatagtatat ttacgaatcg aatctgattt taaggcgaat attttgttag
     2521 tcgcgaccat ttcatggcac cgacctgcgg tcgcagaggc gtgagcttgc cttgacgcgc
     2581 agctcgtgct gacaaagatc atactatgcc cggtgtgaga ctgaaaaaag accagatggc
     2641 ctggatttgt gatccagcgt ggattggctt tgatacttcg gatcaaattc cagatggtcc
     2701 cgggactttc gggcagaacc gtgcgcgcga tgcaattgag tttgcggcca atatgggtcg
     2761 accgggttac aacacggcgg tggttggaga gccaggaagc ggcagacatt ttcttgcgcg
     2821 cagactcctt gagaagcgcg cagcaacaga agcacctcct gacgattggt gctacgtaaa
     2881 taatttcgag gattctactc ggcccagggt tctcatgctt cctgcgggca ggggagccgg
     2941 actcaaagca gacatgcatc ggtttgcggc tgaactcggt tccactatgt cttctgcctt
     3001 tgaagcggaa gagtaccgcc gtcacattga acagattcaa aacgaactca aagcgcgcga
     3061 agaaacagca ttgcgcgaac tgggggcctc ctccctcaaa gaaggaatcg ctctgatcag
     3121 cactgcgcag ggctttgttt tcacggcgg
//
LOCUS       DAORFF010000478         3147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_20241_length_3147_cov_3.873545, whole genome shotgun sequence.
ACCESSION   DAORFF010000478 DAORFF010000000
VERSION     DAORFF010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20241_length_3147_cov_3.873545"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PKK76_15985"
     CDS             <1..530
                     /locus_tag="PKK76_15985"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNL70031.1"
                     /translation="IVGGKNSAAETALRLTRAGAKVTISYRNAKLPEDSIKYWILPDL
                     LGLIRTGEIDFIGESHLLAIEPGKVKLKTRTGEIDKHFEMVLLLTGFEIDPTLYHRCG
                     INTDPQTNRPHWNEATMESNVPGIYLAGTGVAGTQSPYRFFIENCHEHVEIILSRLTG
                     KKTRVFKKFFAMPES"
     gene            complement(537..1796)
                     /locus_tag="PKK76_15990"
     CDS             complement(537..1796)
                     /locus_tag="PKK76_15990"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HNL70032.1"
                     /translation="MKKAILYLNSILLSVALVAGGIWNFVSHNTDSAVTKESRKLTDW
                     PSWDLNALSEGKWTRQVEDAFADRFMFRSHAMALAQKTESLRGLPGEEVKLVTQKSGD
                     LFNEQAITKADKSGTTDAAGAPPAGQWVNQMLIIGKAAYGLVGYSDEGGSYYAQAVNT
                     LASKMGPTIKMYSILVPTAIEFLDDERYTKLSYPQEKGIGLMYGKLHERVKGINVVPA
                     LREHRDEYIYFRTDHHWTARGAYYAYRAAGASMGYTALPMSSYQREEVPGFVGSLTYQ
                     THAAVIENNPDVCEIFYPPGEHTFTRYYSGGVLGGKAIDKAYMLGSNRYTAFIAGDVP
                     RSVAKSTNMNGRKILILKDSYGNAFIPFLIPHFQEIHIIDPRYWDGSLPAYVKEQGIT
                     DVLVFNCFQIVSYYGGFAMNLLRVTGK"
     gene            complement(1825..>3147)
                     /locus_tag="PKK76_15995"
     CDS             complement(1825..>3147)
                     /locus_tag="PKK76_15995"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNL70033.1"
                     /translation="NWVLIVASLIFYAWGEPVWVVLLVLSSLVDYLNGLWIARFRGKA
                     AAKLSVVCTLIFNLGILAIFKYQAFFIHNLNLVSGLSIPVPEHHLPIGISFYTFQTIS
                     YVIDVYRDEVPAQKDFSKFLLFVSLFHQLVAGPIVRYKDIAYEIENRIFSWNDFSYGV
                     SRFAVGLAKKVVLANPAGEIAGSFLDGSPERMSVLGSWWGILLFTFQIYFDFSGYSDM
                     AIGLGKMFGFTYKENFNYPYLARSATDFWRRWHISLSSFFRDYVYIPLGGNRTRPLVS
                     LTVVWFLTGLWHGASWNFVVWGLFYGALILFERAVQSVTGREIPRIIGHPYAILATLV
                     GWTLFYFTDLGHAFAHLGRMFGVSGASLTDPAVLISIRANAFFVGLLILACFPVWPFL
                     AKLLRGDPSESGHARLGVLLPVLNLFLVVFSTVLLVGRTYNPFLYFRF"
BASE COUNT          914 a          803 c          728 g          702 t
ORIGIN      
        1 tgattgtagg aggcaagaac tctgccgcag aaaccgctct gagactgact cgcgccggcg
       61 cgaaagtgac catcagctat agaaatgcaa agctgcctga agattctatt aaatactgga
      121 ttcttccgga tcttctaggg ctcattcgta caggagaaat cgatttcatc ggagagtcgc
      181 atttgcttgc gatcgagcct ggaaaagtga aactgaaaac cagaacggga gaaatagaca
      241 agcattttga aatggtactg ctcttaactg gttttgaaat tgatccgact ctctaccaca
      301 gatgtggaat caatacagac ccacaaacca atcgccctca ctggaacgag gccaccatgg
      361 aatccaatgt cccaggaatc tatctggcgg gaacaggcgt agcaggaact caaagcccgt
      421 atcgcttctt tattgaaaat tgccacgaac acgtagagat cattctatca cggcttaccg
      481 ggaagaagac acgcgtattc aagaagttct tcgcaatgcc ggaaagctaa gacgcctcac
      541 ttccctgtaa cacgcagcag attcattgca aagccgccat agtaactgac aatctgaaaa
      601 caattgaata caagaacatc cgtaatgccc tgctctttta cgtatgctgg caaagatccg
      661 tcccagtaac gcggatcaat gatatggatt tcttgaaaat gcggaatcaa aaacggaatg
      721 aatgcattcc cgtaagagtc tttcaggatc agaatctttc tgccgttcat gttcgttgac
      781 tttgccacag agcgcggaac gtcacctgcg atgaatgccg tataacggtt cgatccaagc
      841 atgtatgcct tgtctatcgc cttcccgccg agcacacccc cgctatagta gcgagtgaat
      901 gtatgctccc cgggtgggta gaaaatctcg catacgtcag gattattctc aatcactgca
      961 gcatgagtct gataggtaag agaaccaacg aatcctggca cttcttccct ctggtaggag
     1021 gacatgggca gagcggtgta tcccatggaa gcacctgcgg ctctgtatgc ataatacgca
     1081 ccgcgcgcag tccagtgatg gtccgttcta aaatagatgt actcatcacg atgctctcga
     1141 agcgcgggca ctacgttgat ccccttcacg cgctcatgga gctttccgta cattaaaccg
     1201 attccctttt cttgaggata ggatagcttt gtgtaacgtt catcatccag aaattcaatc
     1261 gctgtaggca caagaatcga atacatcttg atggtcggcc ccatcttcga tgcaagagtg
     1321 ttcactgcct gcgcgtagta cgatccacct tcatccgaat acccaaccag accataggca
     1381 gcctttccaa tgatcagcat ctggttcacc cactggccgg caggtggagc gcccgcagca
     1441 tccgtggttc cagatttatc agctttcgtg atagcctgtt cgttgaagag gtccccgctc
     1501 ttctgtgtga cgagctttac ctcttctccc ggaaggccgc gcaacgattc tgttttctga
     1561 gcaagagcca ttgcatggct gcgaaacatg aagcgatccg cgaatgcatc ttctacctgt
     1621 ctggtccatt tgccctcgct gagagcattt agatcccagc tgggccagtc ggttaatttg
     1681 cgtgattctt ttgtgaccgc cgaatcagtg ttatgcgata caaaattcca gatccctccg
     1741 gcaaccagag ccacagaaag cagaatggaa ttgagataga gaattgcctt tttcatagat
     1801 tcagtctttc aaatttcagg atcatcaaaa gcgaaagtat aggaacggat tgtaggttct
     1861 tcctacaagg agcaccgtag aaaacacgac cagaaataaa tttaagacag gcaaaagcac
     1921 gcccagcctg gcatgccctg actccgatgg atctcctctc aaaagcttcg caagaaatgg
     1981 ccaaacagga aagcatgcaa gaatgagcag acccacgaag aatgcattgg cccgaatcga
     2041 gatcaaaaca gccggatccg tgaggctcgc tccggacaca ccaaacattc gccccagatg
     2101 tgcaaaggcg tgccccagat ctgtgaagta aaaaagagtc cagccaacga gagtggccag
     2161 gattgcatac ggatgcccga tgattcgcgg aatttcccgt ccggtcacgg actgaaccgc
     2221 tcgctcaaaa agaatgagcg ctccatagaa aagtccccat actacaaaat tccagctcgc
     2281 cccatgccag agcccggtca agaaccacac tactgtgaga ctcaccagag gccgtgtgcg
     2341 attgccaccg agtggaatgt atacgtagtc gcgaaagaag gaactgaggc tgatgtgcca
     2401 gcgtctccag aaatcagtgg cagagcgcgc gagataggga tagttgaagt tctctttgta
     2461 agtaaaacca aacatttttc ccagaccgat cgccatgtcc gaataaccgg aaaaatcaaa
     2521 atagatctga aacgtaaaaa gtaaaattcc ccaccaggaa ccaagcacac tcatgcgctc
     2581 cggagatccg tcgagaaacg agcccgcgat ctctcctgcc gggttggcca gtaccacttt
     2641 cttcgcaaga cccactgcaa atcggctcac gccgtaagaa aaatcattcc atgaaaaaat
     2701 tcgattctcg atttcgtatg cgatgtcttt gtaccgtacg attggtccgg cgacgagctg
     2761 atgaaataga ctgacaaaga gcaagaattt tgaaaaatct ttctgcgccg gcacttcatc
     2821 tcgatataca tctatgacgt aggaaatcgt ctggaatgtg taaaaagata tcccgatagg
     2881 cagatgatgc tcaggaacag gaatcgaaag accgctcacc aggtttaggt tgtgaatgaa
     2941 aaaagcctga tacttaaaaa ttgcaagtat tcccagattg aagatcaagg tgcatacaac
     3001 agaaagtttt gcagcggcct ttccgcgaaa acgcgcgatc cacagcccgt tgagataatc
     3061 taccagagaa gaaagtacaa ggagcactac ccagacaggc tcaccccagg catagaagat
     3121 caaagacgcg acaatcagaa cccagtt
//
LOCUS       DAORFF010000479         3129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_20419_length_3129_cov_3.453155, whole genome shotgun sequence.
ACCESSION   DAORFF010000479 DAORFF010000000
VERSION     DAORFF010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20419_length_3129_cov_3.453155"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PKK76_16000"
     CDS             <1..451
                     /locus_tag="PKK76_16000"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HNL70034.1"
                     /translation="MDFPGFPNVVRIGVFSSGVAADSSNYAKETVCQLTFNVDDQTGE
                     ETGRFIDRAMKEGALDIQVIPATGKKGRPSMVIQLLAREEQKDRMIDLVLRSTRSFGV
                     RVEKVERYRLETRFEERDVQGSKVKYRIGTNLAGAFIKEKPEFDDLP"
     gene            460..1341
                     /locus_tag="PKK76_16005"
     CDS             460..1341
                     /locus_tag="PKK76_16005"
                     /inference="COORDINATES: protein motif:HMM:NF026306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4505 family protein"
                     /protein_id="HNL70035.1"
                     /translation="MREYFYRIDSRGRVIHDGTEISDEGFLNFFFERLRDNDTGLHSD
                     FSFVSPCGNEMNFIKPDLFPIVFSKRSGDRLFYAPGLSVALDLNRMRYSEKLILHPAP
                     IGTFGLLGADLALEFGRDIEPWGPFLSYRVGRSPPAMADSMFVIEPLERSAGTEHLEI
                     IRSDPSVQCFGCGGANAAGLALPFIFDPRANTARSWFKPPAWMAGHPSWMHGGFISLL
                     LDEVCAKVLRGLQRKGATVELNVAFRKPVPLGHEIELLAKLETIEGRRIRVQGSILAE
                     GHVRAEAQALFVELLSA"
     gene            complement(1308..1970)
                     /gene="tmk"
                     /locus_tag="PKK76_16010"
     CDS             complement(1308..1970)
                     /gene="tmk"
                     /locus_tag="PKK76_16010"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNL70036.1"
                     /translation="MPCSIPQITPVPGLFVVVEGIDGSGKSTLVTGLGKALVDHAVLS
                     LCEPTKLASGLLIRRHLTDHSPLPQEAWLEMFGADRRIDLETNINPALARGEIVLLDR
                     YLYSTAAYQGSAGKLNAKEILHSQTNQFRQPDVLIYLDISPEDALARVSATREKLEIF
                     ETSHELHRIHSNYEEILQEVAQEQAGPTLIRIAATDSLDMNIAKAEKAIRQALSNSTK
                     RA"
     gene            2042..>3129
                     /locus_tag="PKK76_16015"
     CDS             2042..>3129
                     /locus_tag="PKK76_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70037.1"
                     /translation="MGRIYQVIFCLVGLANFALVLFDATYLWKLPYTRLTARDLYLAH
                     APDLVRRYDPVKGIDAHRFTTAYLAQADHAFELSQAGNYKDAEKVYADLAAMSREVID
                     QRPGFSHFSIAEKDGTLQVIKNAMRGHFGIESAKDSFARYWSRENLALDRIAAEKGFF
                     DREIRPLMAQNYFRWIDEDGEMRDYFYRIDLWFVAFFLVDFLARWVIAIRLGRHRKWY
                     MFPVRHGVEIFNLVPPHHAVWMRLLRAIPLYLRMKKAGMIPGEGIMPEILHDNAALIA
                     EEISGLVLVNILDQLAVMVRDANPSGIVQSEVAQVTKDLLDSQLDRIADTVVPDVEPQ
                     ITGLVIHSVYQAMGPYLRTPLGPAIRLG"
BASE COUNT          675 a          751 c          875 g          828 t
ORIGIN      
        1 tatggacttt cctggttttc caaacgtcgt tcgcattggt gtgttttcta gtggtgttgc
       61 ggccgattct tccaactatg cgaaggagac agtatgccag ctaacgttta atgtggacga
      121 tcaaacggga gaggaaaccg gacgattcat tgatcgcgca atgaaggaag gtgcgctgga
      181 cattcaggtg atacctgcga ccggaaagaa gggcagacct tctatggtga ttcaattgct
      241 tgcacgtgaa gaacaaaagg acaggatgat cgatcttgtc cttcgcagca cacgttcctt
      301 tggtgttagg gtggaaaagg ttgagcgtta tagattggag acccggtttg aagagcggga
      361 cgtgcagggt agtaaagtaa agtatagaat cggaacgaac ctggctggag cattcataaa
      421 agaaaagccg gagtttgacg acctgccgta gttcagcata tgcgggagta cttctataga
      481 attgattcgc gtgggcgagt gattcatgac ggcacggaga tttcggatga aggatttctc
      541 aatttcttct tcgagagact gcgcgacaac gatacgggac tccacagtga tttttcattt
      601 gtctctcctt gcggcaatga aatgaatttc ataaagccag atctttttcc gatcgttttt
      661 tcaaaacgat ccggagatcg tctcttctat gcgcccggtc tttctgttgc actcgatcta
      721 aataggatgc gatattctga gaagctgatc cttcatcctg cgccgattgg cacctttggg
      781 ttgttaggcg ctgatcttgc gctcgaattt ggccgggata tcgaaccatg gggtccgttt
      841 ctgtcctatc gcgttggacg gtcgcctcct gccatggcag attcaatgtt tgtgatcgag
      901 cctctggagc gatcggcagg gaccgaacac ctcgagatca ttcggtcaga tccttcggtt
      961 caatgctttg gatgcggcgg tgcaaacgca gctggattgg cgttgccttt catctttgat
     1021 ccgcgagcca acactgcgcg aagctggttt aagcctccag cctggatggc cgggcatccg
     1081 tcctggatgc acggcggttt cataagtcta ctgctggatg aggtttgcgc gaaggtgctt
     1141 cgcggattgc agagaaaggg agcgacggtc gagctcaatg ttgcgttccg taaacccgtg
     1201 ccgctggggc atgagattga actgctggcc aaactcgaaa ccatagaagg tagaaggatt
     1261 cgggtgcagg gttccatcct cgccgaaggg catgtgcgag cagaagctca ggctcttttc
     1321 gtggagttgc tcagcgcctg acggattgct ttttctgctt tggcgatatt catatccagc
     1381 gagtctgttg cggcgattct aatcaacgtg ggacccgcct gctcttgtgc tacttcctgt
     1441 aggatctcct catagttcga gtggatcctg tgcaattcgt ggctggtttc aaatatttcg
     1501 agtttttctc tggtagcgct cactcgggcc agggcatctt cgggcgatat atcgagatag
     1561 atgaggacgt ccggctgtcg gaattgattc gtctgggaat gtaggatctc cttcgcgttc
     1621 agttttccgg cggatccctg gtaggccgct gttgagtaaa gataccgatc caggagaacg
     1681 atctcgcctc tagccagagc aggattgata ttcgtttcca ggtcgatcct tctgtctgcg
     1741 ccgaacattt cgagccacgc ctcctggggc agcggtgaat ggtccgttag atgcctgcgt
     1801 atgagcaacc cggatgcgag tttcgtgggc tcgcatagcg aaaggaccgc atgatccacc
     1861 agggctttcc cgagtccagt tacaagagtg ctctttcccg atccgtcaat cccctctacg
     1921 actacaaaca accctggcac aggagtgatt tgtggaatag agcagggcac tcaagcgttt
     1981 ttggcggaaa gtgtttgact gcagatcgtt ccgcaccgac gaacagaaag tcatgagact
     2041 tatgggacgc atttatcagg tgattttctg ccttgtaggc ctggccaatt tcgcgctggt
     2101 tttgtttgat gctacctatt tgtggaaact tccctatact cgtttgactg ctcgcgacct
     2161 gtacctggct cacgcccccg atcttgttcg acgttatgat ccggtgaaag gaatcgatgc
     2221 gcatcgtttt acaaccgctt acctggccca ggcggatcac gccttcgagt tgtcccaggc
     2281 cggaaattac aaagatgcag aaaaggtgta cgccgacctg gcggccatga gccgcgaagt
     2341 gattgatcag cgtcccggat tcagtcactt cagcattgcg gaaaaagatg gtactttgca
     2401 agtaatcaag aacgcgatgc gcggtcactt tggcattgag tctgccaagg actcatttgc
     2461 gcgctactgg tccagggaga atctggcact cgatcggatt gcagccgaga aagggttctt
     2521 cgacagagaa attcgaccct tgatggccca gaactatttc agatggatcg acgaagacgg
     2581 tgagatgcgc gattacttct ataggatcga tctctggttt gtggcattct tcctggtcga
     2641 ttttctggcg cgctgggtga ttgcgattcg cctgggtcgt catcgcaaat ggtacatgtt
     2701 cccggttcgc catggcgttg agattttcaa tctggttcct ccccatcatg cagtgtggat
     2761 gaggctcctt cgtgccattc cattgtattt gagaatgaaa aaagccggga tgataccggg
     2821 cgaaggcatc atgccggaga tcctacatga caacgccgcc ttgatcgcag aggagatttc
     2881 cgggctcgtt cttgtaaata tccttgatca gctggctgtt atggttcgag atgcaaaccc
     2941 ctcaggaatc gtccagagcg aggttgctca ggtgacgaaa gaccttctgg attctcaact
     3001 tgaccgcata gcggacacgg tcgtccccga tgttgagccc cagattaccg gccttgtgat
     3061 tcattccgtg tatcaggcta tggggcccta tttgcgtacg cctcttggcc ctgcgattcg
     3121 actgggcat
//
LOCUS       DAORFF010000480         3120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_20520_length_3120_cov_3.893964, whole genome shotgun sequence.
ACCESSION   DAORFF010000480 DAORFF010000000
VERSION     DAORFF010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20520_length_3120_cov_3.893964"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..774
                     /gene="folE"
                     /locus_tag="PKK76_16020"
     CDS             190..774
                     /gene="folE"
                     /locus_tag="PKK76_16020"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNL70038.1"
                     /translation="MQAEDQGRADKLTEQYHGILQSLGENPSREGLLKTPERAAKAML
                     FLNSGYKMKLGDLVNDAVFSDVNQGMVIIRDIEVYSLCEHHLLPFFGQVHVGYIPDSK
                     IIGISKIPRIVDMFARRLQVQERLTEEILGALNQVLSPVGAGVVMQCKHMCMMMRGVE
                     KQNSVMFTSSMSGTFLTDRATRMEFLDLIRTSKN"
     gene            832..1491
                     /locus_tag="PKK76_16025"
     CDS             832..1491
                     /locus_tag="PKK76_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70039.1"
                     /translation="MLRRLLPFLILALIFDCKSSSDEASGLLVGPAALSIGGIYSFYG
                     KSVDKTYTTSCGTAGTAFATTSSSSTSTAATTTTSSSSKKTEQTSYGIQSYYVFSNGE
                     TMSLKYDYYTTRDTFSFTPLNAATTSCNTDDLTTCNSTGRFTCTTSDNLNCGGTYAFL
                     FRNTIPALAFQGLSGSVSWKKGFSLNSSNNAVNNVVLTYNMISADGSVFKGTVECNIA
                     Q"
     gene            1542..2678
                     /locus_tag="PKK76_16030"
     CDS             1542..2678
                     /locus_tag="PKK76_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNL70040.1"
                     /translation="MRVLSVILPTYNESRNVPILIEKLGVALAGIDYEVLVVDDNSPD
                     RTYEIVDNISKTNPRVRCIRRIHERGLSSAVVTGMSASEAQYFAVMDADLQHDESILP
                     DLLRAVREEGYDLAVGSRAAEDGSFGEWSRVRRFMSWSATLMAKILLPVQVKDPMSGF
                     FLTSRGVFEKARDQINPVGFKILLEFLGRLRGLKVKEVGYTFRTRIHGETKLSGSVIR
                     NYLVALLDLRFGHVASPVFILYCLVGASGVIVYYAVRALTDHIMISFLDTGLAPPFAR
                     IPAGAILAFIFSVTNNYAWNNYLTFYDIRHKGFRIATGVILFFLVSLVGLLVQTAVTH
                     LIHTLGMPADAASAAFARAAGISVAVITNYFLNVTFTWRARRIA"
     gene            complement(2675..>3120)
                     /locus_tag="PKK76_16035"
     CDS             complement(2675..>3120)
                     /locus_tag="PKK76_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70041.1"
                     /translation="TLFSSVSDLRERMERQQDFAFFPVGFQESPSTKTFAVLTETSQH
                     IQSFQVQCFRALDAMSSDSLRITHSGRTLLLASAALIRKRPEIAEGASLILLQDVDTS
                     AVDVYEATRHLAARVVFTDHAPALSDREASMQPFTSASELDQMEV"
BASE COUNT          784 a          833 c          795 g          708 t
ORIGIN      
        1 aagaatctgc ccatgcgagc gcgcgatgcg gtcatcttgt acggagagga tccagaagac
       61 caggggattc tctaccagga tctggatggt tcgattcgct ttcgccgcga ggcgttcacg
      121 gagtgggaaa agaaaataca gggcctcgca gacaggctca ccaggtagga aacagtggac
      181 atagattcca tccaggcaga agaccaggga agagcagaca aacttacaga gcaataccac
      241 ggcatcctcc agtccctggg agaaaaccct tcgcgcgaag gattgctcaa gactccggaa
      301 cgcgcggcca aggccatgct ctttttgaat tctggctaca agatgaagct gggcgatctg
      361 gtgaacgacg cagtcttctc ggacgtaaac cagggaatgg taatcattcg cgacatagaa
      421 gtgtacagcc tgtgcgaaca ccaccttctg cccttcttcg gccaggtgca cgtagggtat
      481 atcccggata gtaagatcat tggaatttca aaaatcccgc gaatcgtcga catgttcgca
      541 cgcaggctcc aggtccagga acgcctgacg gaggaaatcc tcggtgctct caaccaggtt
      601 ctctctccag tgggggccgg agtcgtcatg cagtgcaagc atatgtgcat gatgatgcgc
      661 ggagtagaaa agcaaaactc ggtcatgttc acaagtagca tgagcggcac atttctaacg
      721 gatcgggcta ccagaatgga gttcctggat ctcataagaa cctcaaaaaa ctgaagaaaa
      781 ctgctggcag agctccatgg agattggagg atcagactcc gacaatagaa tatgctccgt
      841 agactgctgc cattcctgat tcttgctttg atttttgact gcaagtcctc cagcgatgag
      901 gcttcgggtc tgctggttgg accggccgcc ctctccatcg ggggcatcta cagtttctac
      961 ggaaagagcg tagataagac ttacaccact agctgcggaa cggcaggaac tgcgtttgcg
     1021 accacgagct caagcagcac ttctacggca gccacgacca ccacgagcag cagctcgaaa
     1081 aagacagagc agaccagcta cggaattcag agttactacg tgttctccaa tggagaaacc
     1141 atgagcctga agtatgatta ctacactacg cgcgatacgt ttagcttcac cccactcaac
     1201 gcggccacca cttcctgcaa tacggacgat ttgaccacgt gcaatagcac gggcagattc
     1261 acctgcacga cctcagacaa cctcaactgc ggtggaacct acgcctttct atttcgcaat
     1321 acgatccccg ccctcgcctt ccagggtctg agcggttctg tctcctggaa aaaagggttc
     1381 tctctcaatt cctcgaacaa tgcagtcaac aacgtagtgc tcacctacaa tatgatcagc
     1441 gcagacggca gcgtcttcaa gggcacagtg gaatgcaaca tcgcccagtg attcttgttt
     1501 aattttgtcg ccctgaacga gccttggaat atcctgggcg cgtgagagtc ctctccgtca
     1561 ttctacccac atacaatgaa tccaggaacg tccctatcct cattgagaag ttaggcgttg
     1621 cactggctgg catagactac gaagttctag tcgtagatga caacagcccc gatcgcacgt
     1681 acgaaatcgt agacaacatt tccaaaacca atccgcgtgt tcgctgcatt cggcggatcc
     1741 acgagagagg actttcttcc gcggttgtca ccggaatgag cgcatcagaa gctcaatact
     1801 ttgctgtgat ggatgcggac ctccagcacg atgagtccat ccttcctgac ttgcttcgcg
     1861 cagtcagaga agaaggatac gatctggcag tcggctcgcg cgcagcagag gacggaagct
     1921 ttggcgaatg gtcccgtgtg cgaagattca tgagctggtc tgcgactctc atggcaaaga
     1981 tacttctgcc tgttcaggta aaagatccaa tgagtggctt cttcctcacg tccaggggtg
     2041 tattcgaaaa ggcgcgcgac cagatcaatc cagtcggatt caaaatcctg ctcgagttcc
     2101 tgggcagact ccgcggtctg aaagtaaaag aagtaggtta cacgtttcgc acacgcatcc
     2161 acggagaaac aaaactgagc ggatccgtca tacgcaatta cctcgtggct cttctggatc
     2221 tcagatttgg tcatgtggcc agtcctgttt tcattctcta ttgcctcgtg ggtgcttccg
     2281 gcgtgattgt ttactatgct gtgcgtgcac tgacagatca catcatgatc tcttttctgg
     2341 acaccggtct ggcaccgccc ttcgcgcgca ttccggctgg tgcaattctc gctttcatat
     2401 tcagcgtcac aaacaactat gcgtggaata actatctgac tttctacgat atcagacaca
     2461 aagggtttcg catcgcaaca ggagtgatcc tcttcttcct ggtgagtctg gttggtttac
     2521 tggtccagac cgcagtaact catctcatcc acactctcgg catgcctgca gatgcggcaa
     2581 gtgcagcctt tgcgcgagcg gccgggattt ccgtagcggt gattacaaat tacttcttga
     2641 acgtgacttt cacctggcgt gcgcggcgaa tcgcctagac ttccatctgg tccagttcac
     2701 tcgcagatgt aaatggctgc atagacgcct ctctatcaga gagagcagga gcatgatctg
     2761 taaacacaac tcgcgccgcc agatgcctgg ttgcttcgta gacatccacg gcagaagtat
     2821 ccacatcttg cagtagaatc aaagaagccc cttccgctat ttctgggcgc tttcgaatga
     2881 gtgccgcact ggcgagtaag agagtcctgc cagaatgagt gattctcaga gaatccgagg
     2941 acatggcatc gagcgcgcga aagcattgca cctgaaacga ctgaatgtgc tgcgaagttt
     3001 ccgtgagaac tgcaaacgtc ttagtagaag gactttcttg aaaccccacg gggaagaacg
     3061 caaaatcttg ctggcgttcc attctttcgc gcaggtcgct cacagatgaa aaaagagtga
//
LOCUS       DAORFF010000481         3111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_20616_length_3111_cov_4.196335, whole genome shotgun sequence.
ACCESSION   DAORFF010000481 DAORFF010000000
VERSION     DAORFF010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20616_length_3111_cov_4.196335"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PKK76_16040"
     CDS             complement(<1..796)
                     /locus_tag="PKK76_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNL70042.1"
                     /translation="MKTDHLILDESGKQTRDKPWIFRTYAGHTSARASNELYRTNLAA
                     GQTGLSIAFDLATQCGYSSDHPIAKPEVGKVGVPINSLDDFHILFDQIPLEKMNTSMT
                     INATSMWLLSLYIALARERNESPKKLQGTTQNDIVKEYLARGTYIFPPQASLRMIAEM
                     YEYCLKEVPEWNPSNICSYHLQEAGATPTQELAFALANAIGLLDLIRERGTFTHDEFE
                     KCVGRISYFVNAGIRFVEEMCKMRAFTELWEEITRDRYGVKNAKYRM"
     gene            complement(848..2524)
                     /gene="ilvD"
                     /locus_tag="PKK76_16045"
     CDS             complement(848..2524)
                     /gene="ilvD"
                     /locus_tag="PKK76_16045"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNL70043.1"
                     /translation="MNLNWNSKQITQGDRRAPNRAMMRAVGFEDSDFDKPVVGVASGH
                     STITPCNAGIRPLEEAACKGISSAGGMPQTFGTITISDGISMGTQGMKLSLVSRDVIA
                     DSIETVCRGQLMDGVLAIGGCDKNLPGAMMAIARLDIPGIFVYGGTILPGKYQGRDLN
                     IVSVFEAVGSFQAGTISREELNEIEKHACPSSGSCGGMYTANTMSSAIEALGMSLPNS
                     SQMSAPSPEKKSSAEESGEALVRMIRTNLRPSQIMTRKAFENAIAVVMATGGSTNAVL
                     HLIAIAHDMGITLTLDDFEAISKKVPHLADMRPSGKYLAVDFHRAGGVPLIMKLLLKA
                     GILHGECMTVTGRTVSENLAEVPDAPPAGQDVIRPFSSPVYKRGHLVILRGNLAPGGA
                     VAKTSGVKSPAITGEARVFDSEEECMDAIMSDQIKPGDILVIRYEGPKGGPGMREMLS
                     PTSALIGKGLGDSVGLITDGRFSGGTYGMVVGHVSPEAAVGGPIALVREGDVITIDAY
                     ENLLQLHMTEAEMEARRKEWVKPESPVKNGVLYKYAKLVGSAEFGAVTDA"
     gene            complement(2640..>3111)
                     /locus_tag="PKK76_16050"
     CDS             complement(2640..>3111)
                     /locus_tag="PKK76_16050"
                     /inference="COORDINATES: protein motif:HMM:NF017893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HNL70044.1"
                     /translation="GVDFFVPVDIYAQIERASKYAMLFISLTFLTFFVFEAVAGLQIH
                     PVQYLFVGLALSLFFLLLLSLAEHIGFARAYGLASVADLGLITAYSLKILKSRTRAGI
                     LLGVLAGLYSFLYVVLQLEMYSLLAGSIGLFLILAIVMYATRNIDWYAKEPAKA"
BASE COUNT          728 a          809 c          783 g          791 t
ORIGIN      
        1 acattctata ttttgcattt ttcactccgt agcgatctcg agtgatttct tcccacagct
       61 ccgtgaatgc gcgcatcttg cacatttctt ccacgaagcg aatccctgcg tttacgaagt
      121 agctgattct tcccacgcat ttttcaaatt cgtcatgcgt gaacgttcct ctttctcgga
      181 tgagatcgag cagcccaatt gcattggcca gagcgaatgc aagctcctga gtgggagtgg
      241 cacctgcctc ctgcaaatgg taagagcaaa tgttagaagg gttccattct ggaacttctt
      301 tcaaacagta ttcatacatc tctgcgatca tgcgcagaga ggcctgagga gggaaaatgt
      361 atgtgccgcg cgccaggtac tctttgacaa tgtcgttttg agttgtgccc tgcaattttt
      421 ttggactttc attcctttcg cgcgcaagtg caatgtagag agacagaagc cacatggacg
      481 tggcgttgat ggtcatagaa gtattcatct tctcgagtgg aatctgatcg aagagaatgt
      541 gaaagtcatc gaggctgttg atgggcactc ccacttttcc tacttccggt tttgcgatgg
      601 gatgatccga agagtagccg cactgagtgg ccagatcaaa ggcgatcgac aggcctgtct
      661 ggcctgcggc caggttggtt ctgtatagtt cgttggatgc gcgggcgctt gtgtgcccgg
      721 cgtatgtgcg aaatatccag ggtttgtctc tagtctgttt gccggattcg tccagaatga
      781 ggtgatcggt tttcatatgg cgcctgagtc atttttccgg gacccagggc tcccgtgcac
      841 tcaaaaatca tgcatccgta accgccccga attcagcgct tcccacgagc ttagcatact
      901 tgtagagcac tccatttttt accggcgatt ctggttttac ccattctttt ctgcgtgctt
      961 ccatttccgc ctctgtcatg tgcaattgaa gcagattttc gtatgcatcg atcgtgatca
     1021 cgtcgccttc gcgcacgagt gcgatgggtc cacccaccgc cgcttccgga gaaacatgcc
     1081 cgactaccat tccataggtg ccgcctgaga atcttccgtc tgtgatgagg ccaactgaat
     1141 cgccgagacc cttgccaatg agagcagaag taggcgaaag catttcgcgc attccagggc
     1201 caccctttgg accttcgtat cgaatgacca ggatgtctcc cggtttgatt tgatcgctca
     1261 tgatagcgtc catgcactct tcctctgaat caaacacgcg cgcttctcct gtgatcgcgg
     1321 ggcttttgac tccagatgtt tttgcaactg ctcctccagg agccagattg ccacgaagga
     1381 tgaccaggtg acctctctta tacacaggag aagaaaaagg tcgaatcacg tcctggcccg
     1441 cgggaggagc atctggaact tccgccaggt tttctgaaac agttcgcccg gtcacagtca
     1501 tgcattcacc gtgaaggatt cccgccttga gaagcaactt catgatgaga ggaactccac
     1561 ccgctctatg aaaatcaact gcgaggtatt tgccggatgg cctcatgtct gccaggtgag
     1621 ggactttttt agaaatcgct tcaaagtcgt ctaacgtcag ggttattccc atatcatgcg
     1681 caatggcgat cagatgaagc actgcgtttg tggagccacc tgtggccatg actactgcaa
     1741 ttgcgttttc gaatgctttt cgcgtcatga tctgagacgg gcgcaggttg gttcgaatca
     1801 tgcgaacgag tgcctctccg ctttcttccg cagatgattt cttttcaggg gacggtgctg
     1861 acatctgcga tgagttcggc aggctcatcc cgagcgcttc aatggcactg gacattgtat
     1921 ttgcagtgta cattccacca cagcttccag aagaaggaca ggcgtgcttt tcaatttcat
     1981 ttagctcttc gcgcgaaatc gtgcctgcct gaaaagaacc aaccgcctcg aatacagaaa
     2041 cgatgttaag atcgcgcccc tggtattttc ctggaagaat ggttccgcca tatacaaaga
     2101 ttcctggaat gtccagacgc gcgatcgcca tcatcgcacc aggcaggttc ttgtcgcagc
     2161 cgccaatggc cagcacacca tccatgagtt gcccgcgaca tactgtttca atgctgtctg
     2221 cgatgacgtc gcgactcacg agcgaaagct tcattccctg ggtgcccatg ctaattccat
     2281 ctgaaatcgt tatggtgcca aacgtctggg gcatcccgcc agcggatgaa atccctttgc
     2341 atgcagcttc ttccagaggt cgtatgcctg cgttgcaggg agtgattgtg gaatgaccgc
     2401 ttgccacacc tacgactggt ttgtcaaagt cgctgtcctc aaaacctaca gcacgcatca
     2461 tcgcacggtt gggcgctcgg cgatctcctt gtgtgatttg ctttgaattc cagtttaagt
     2521 tcataggttc cacagagccc ggacggcttc ggggtcaagt aatcgagggg gaggtcacgc
     2581 gggcataaaa aaagccgccc cgcgaagggc ggcaatggtt ggttcaatgg aagaatgtct
     2641 tacgctttcg ctggttcctt tgcgtaccag tctatgttgc gggttgcata catcacaatt
     2701 gccaggatta agaacaggcc aatggatcct gcaagcagag aatacatttc tagctgcaac
     2761 accacataca ggaaggaata aagtcctgcc agaacgccga gcaaaatgcc agctctggtt
     2821 ctggatttca aaatcttcaa gctataggcc gtaatgaggc caagatctgc aacagacgcc
     2881 agcccatagg ctcgcgcaaa tccaatgtgt tctgccagag acagtagaag caggaagaac
     2941 agactcaaag ccaggcctac gaaaaggtac tgcacaggat gaatttgaag ccccgccacc
     3001 gcttcgaaaa cgaaaaacgt gagaaatgtg agcgatataa aaagcatcgc gtatttggac
     3061 gctcgttcaa tttgcgcgta aatatctact ggcacaaaga agtccactcc g
//
LOCUS       DAORFF010000482         3108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_20662_length_3108_cov_3.021291, whole genome shotgun sequence.
ACCESSION   DAORFF010000482 DAORFF010000000
VERSION     DAORFF010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20662_length_3108_cov_3.021291"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PKK76_16055"
     CDS             <1..582
                     /locus_tag="PKK76_16055"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNL70045.1"
                     /translation="SHPTVPINYYGFTKKSVEENLSWFSRLRGMRFAALRYFNAAGYD
                     RSGRIRGIERRTQNLLPLVMEVAAGIRPQISVFGTDYDTRDGTCIRDYVHVEDLASAH
                     VLALEYTARERKDVIVNLGSELGMTVFEMIRIAEEVSGKKIKFETAARRAGDPPSLVA
                     SSTLGKNLLGWSARYSDARTIIESMWNLYRDLK"
     gene            555..1427
                     /locus_tag="PKK76_16060"
     CDS             555..1427
                     /locus_tag="PKK76_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL70046.1"
                     /translation="MESVQGSEMSPVRFSGLLSFIAVLLFAACGPSSRIQEGDVLLAQ
                     NKFDAALLSYQRAYLGSPDHPLAKVALGRMLTLRRVSFYAGMDLMKAAYDQKPTASLR
                     RELVLLHTEAGDIERAEAILHPDHMALDEYMTESAGIERAFVTCIRKQNEEGFYPLSN
                     RASDIEQTKEGFQIRCLLSPGWRRSKLEDAKKIFATLKSPRLQCELIVLFPELSDKKA
                     DQCRKEFPGVISVHRENIRGIESQKGRTANLFEDDLFIPGDPPPQPKEEDAGPFVTAP
                     PASPGTTPAPAVTP"
     gene            complement(1458..1880)
                     /locus_tag="PKK76_16065"
     CDS             complement(1458..1880)
                     /locus_tag="PKK76_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70047.1"
                     /translation="MPDWIVWAPMLAAVGCATIAKATLHREMRKLTQAQIQTWSTEFM
                     TQRMWQFGGIIAVLCIILYVSSGRADRTQALMGLAGAGALIALWLPFSLLSTRKAMMK
                     LELPASFQRAVLIDKVMQGLLLGLFLFYGFKNAGFTSL"
     gene            1977..3047
                     /gene="carA"
                     /locus_tag="PKK76_16070"
     CDS             1977..3047
                     /gene="carA"
                     /locus_tag="PKK76_16070"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNL70048.1"
                     /translation="MVLENGSTFRGFSFGAESEALGEVCFNTSLTGYQEILTDPSYKK
                     QIVTLTYPMIGNYGVEPLAEQSARIQASGLIVKEYVRIPSNFASKSSLSDYMKEQNIP
                     GLEGIDTRRLVLMLRNEGAMRGGIFPDQTDTSSLVAKVKSIPEMTGLDLAREVTTAKS
                     YKFGDHKSGKLSIAVLDFGVKTGILRLLDQNGFNVTVFPASTRYAELKDQNFDCYFLS
                     NGPGDPEPLDYAIDTARKIIEDGKPVFGICLGHQVLGLASGRKTYKLKFGHRGGNQPV
                     KNLESGRVEITAQNHGFAVEEDKGTSPLALSRVNLNDQTVEGFGDPARKILSIQYHPE
                     ACPGPHDAEYLFAKFYEMVKGK"
BASE COUNT          792 a          744 c          802 g          770 t
ORIGIN      
        1 agccatccta ctgtccccat caattactat ggctttacaa agaaatcagt ggaggagaat
       61 ctctcgtggt tctcgaggct tcgcggaatg cgatttgccg ctctccggta cttcaacgca
      121 gcaggctatg atcgctcggg gcggattcga ggcatagaaa gacgcactca gaatctgctt
      181 ccactcgtga tggaggtggc agcaggcatt cgtccacaga tttctgtttt tgggacggat
      241 tatgatacgc gcgatggaac ctgcattcgc gactacgttc acgtagaaga tctagcttct
      301 gcgcatgttc ttgctctcga atacacggcg cgcgaaagaa aggacgtgat tgtcaatctt
      361 ggttcagagc tcggcatgac tgtgttcgaa atgattcgca tcgcagaaga agtcagcgga
      421 aaaaaaatca agttcgaaac ggcagctcgt cgtgcgggag atccaccttc tctggttgca
      481 tcttccactc ttggaaagaa cttactgggc tggagtgcgc gttattcgga tgctcgaacc
      541 atcatagaaa gcatgtggaa tctgtacagg gatctgaaat gagtcctgtg cgattttccg
      601 ggctactctc ttttattgcg gttttgcttt tcgccgcttg cggtccttcg agtcgaattc
      661 aggaagggga cgtacttctc gcccagaaca aatttgatgc cgctcttctt tcctatcagc
      721 gcgcttacct cgggagcccg gatcatcctc tggccaaagt ggcgctcggc agaatgctta
      781 cattgcgccg cgtttccttc tacgctggaa tggatttgat gaaggcagca tacgatcaga
      841 aacccacagc ctctcttcgc cgcgagctgg ttctgcttca tacagaagcg ggagatatag
      901 agagagcgga agccattctg catccggatc acatggctct ggatgaatat atgactgaat
      961 ctgcgggaat tgaaagagca tttgtaacgt gcattcgcaa acagaacgaa gaaggattct
     1021 atccattgtc caatcgtgct tccgacatcg agcaaacaaa ggaaggattt caaatacgct
     1081 gcttgctttc gccgggttgg agaagaagca agctcgaaga cgccaaaaag atttttgcaa
     1141 ctctcaaatc gcccagattg cagtgcgaac tgattgttct ttttcctgag ctatcagata
     1201 agaaagcgga tcaatgcaga aaggaatttc ctggagtgat ctccgttcat cgtgagaaca
     1261 ttcgagggat agaaagccag aaaggtagaa ctgcgaattt gtttgaggac gatttgttca
     1321 ttccaggaga tccaccgcct cagccaaagg aagaagatgc cggtcctttt gtaaccgctc
     1381 cgcctgcgtc tcctggcacc actccggcac ccgctgtcac tccttgattt ggatgccagg
     1441 atcttcggct gtcctgatta gaggctggtg aatcctgcgt ttttgaatcc gtaaaaaagg
     1501 aacaatccca ggagcagacc ctgcatcact ttgtcgatca gcaccgccct ctggaaggaa
     1561 gccggtagct caagcttcat cattgctttg cgcgtggata gtagactgaa cggaagccac
     1621 agtgcgatga gagcgccggc accggccagg cccatgagcg cctgagttct atctgctctt
     1681 ccggaagaaa cgtacaggat gatgcacaat actgcgatga tcccgccgaa ctgccacatc
     1741 ctctgagtca tgaattcagt gctccaggtc tgtatctggg cctgggtgag ctttcgcatt
     1801 tcgcggtgaa gggttgcttt cgcaatggta gcacatccta cagctgcgag catgggggcc
     1861 caaacgatcc aatctggcat aagagcagga attttaaggc gattctgctg gcaatctgga
     1921 tcagttgaca cggtcgttct cacggcaaat ttgacctcgt gagaaagcgc gccattctgg
     1981 tcctggaaaa cggttctaca ttcaggggct tttcattcgg agcagaatca gaggcccttg
     2041 gcgaagtatg cttcaataca tctcttaccg gctaccagga gattttaaca gatccttcct
     2101 ataaaaaaca gatcgtaact ttgacttatc ccatgatcgg gaactacggt gtggaacctc
     2161 tggcagaaca gtctgctcgc atccaggcaa gcggactgat tgtaaaagaa tacgtccgca
     2221 ttcctagcaa cttcgcaagc aaaagcagtc tgtccgatta catgaaagag cagaacattc
     2281 ctggcctgga aggaattgat actcgccgtc tggtgctgat gcttcgcaat gaaggcgcca
     2341 tgcgcggggg aattttcccg gatcagacgg atacgtcttc tcttgtcgcg aaggtgaagt
     2401 ctattcctga aatgacaggg cttgatctgg cacgcgaagt gaccactgcg aaatcataca
     2461 aattcggaga tcataaatcg ggcaaactca gcatcgccgt gcttgatttt ggagtgaaga
     2521 cagggatcct caggcttctg gatcaaaatg gattcaatgt aaccgtattt cccgcatcta
     2581 cccgttatgc ggaattgaaa gatcagaatt ttgactgcta ttttctttcc aatggtcccg
     2641 gtgatcctga accacttgac tacgcgatcg acactgctcg caagatcatt gaagatggaa
     2701 agcctgtctt cggaatttgt ctggggcatc aggtgcttgg ccttgcttcc ggcagaaaaa
     2761 cttataagct gaagtttggt catcgcggtg gaaatcagcc agtgaaaaat ctggaaagtg
     2821 gccgagtaga aattacggcg caaaaccacg gcttcgctgt ggaagaggac aaaggtacaa
     2881 gtccgcttgc actttcgcgc gtaaatctga atgatcagac tgtggaagga ttcggtgatc
     2941 ccgcaaggaa gatactgagc attcagtatc atccagaagc gtgccccggc ccgcatgatg
     3001 cggagtattt atttgcaaaa ttttacgaaa tggtgaaagg aaagtgaaga agatcgtctt
     3061 tcttttgatt tgccttctgg cggcctgtca caccggagat acaaaaaa
//
LOCUS       DAORFF010000483         3093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_20844_length_3093_cov_3.135945, whole genome shotgun sequence.
ACCESSION   DAORFF010000483 DAORFF010000000
VERSION     DAORFF010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20844_length_3093_cov_3.135945"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1739)
                     /locus_tag="PKK76_16075"
     CDS             complement(129..1739)
                     /locus_tag="PKK76_16075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HNL70049.1"
                     /translation="MKHSTAMLRIVTTIFILSTAALAAEDLPVRSVLLYPDRAQITRK
                     DNVKLVAGKNRLIIANVSPNLLPDTLRASCAPDCSIVEVSSRLDRFSETVRPRIQTLE
                     KKFAAVKEKRDTVSATIQRNQFVLNFVNQLSTAASRVIGEETMRPADSGRWTKAAQFI
                     QNERTARRSLIQKQEEQGRKIDLELAAIQKELDMARAEGEKTARTVEVRLTSSRAQSA
                     EVQVGYNIRGASWSISYGALLAKDGSVEIEMFGNAVQSTGEDWKNVSLGFSTANTERG
                     LERPRVRPIRLRVIEKSTAQSYKLEDRATGNVDATESQPAEAEETGGQTDIETGGTAL
                     IFRVPEPQTVESSNRSKKVPLARFVLKPMETYYRVVPSISESAVLAVKLKNDRVFPLL
                     AGPVDLFRGGRFAGKTRLHYVAGTSEFTLGFAEDRNIVVTRTVNRNRGTAGILGGGKL
                     WTREIRFETENRGNTPADIRIFESIPVSELEEVKISVTESGGFAEEKKGSGIYSKRLT
                     LTPGSKGSAMITYTVRTPEDFPDNLIDR"
     gene            complement(1736..>3093)
                     /locus_tag="PKK76_16080"
     CDS             complement(1736..>3093)
                     /locus_tag="PKK76_16080"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNL70050.1"
                     /translation="EADFNLSYRTPGADWYPVYSVRVGRAEKEAVKLSAFALVQNRTG
                     EQWDNVKLQLSASNPEDLISLPELREWRIRAAMIAEQNINDATSDKRRQNISPAAPAR
                     EMQRQSMDEEAQAPGKPSYAPQTQTNQVVSQLPADNKPVNDELNKTVSNSRNTLKESQ
                     GITSGKDASLLSVLNSRDRAFQDGDYDGAAKYSEEFMGKLGKLRNENQKYFATEEKKS
                     REIREKSLTALQNRTNNLIAPRALRGAAIYETQYSETIPSDGILRKVSLFDSDLQAER
                     VYECAPMHQAFGYLTGHVQYKNDKPLLPGPAQIFYGDDFTGEAVLPETGNNQKIALSL
                     GSTEDIKVTRTENRFRESAGLLGGDLRYKIEVQIKIKNSRREMIVLDVFDRVPRSEDD
                     RIKIEETGLTPAPVERTPEGLARFRLNIGPGAEQIVTLKYTLRHSAEITPSGVEAGPA
                     L"
BASE COUNT          609 a          790 c          802 g          892 t
ORIGIN      
        1 caaaagcaat ttgacatcaa cggaatcgca gtatgccatg cgcgctgcgc gtacgcccat
       61 atctttgagc gatagagcgt tctctttttc aatcgggatc cagccgatcc cgaccacact
      121 gaccggcctc atcgatcgat cagattgtcc gggaaatcct ccggcgtacg aaccgtataa
      181 gtaatcatcg cagaaccttt tgatccgggt gtaagcgtga gacgctttga gtaaatcccg
      241 gatcccttct tctcttctgc gaatccgcca ctctctgtca ctgaaatttt tacttcttcg
      301 agttcggaga ctggtatgct ttcaaagatc cgaatgtccg cgggcgtatt gcccctgttt
      361 tcagtttcaa aacgaatttc gcgcgtccag agtttgcctc cacccagaat gcccgcagtg
      421 ccgcggttgc gattcactgt gcgcgtaaca actatgttgc gatcttccgc aaatccgagc
      481 gtaaactcgc tcgttcctgc gacgtagtga agtctggtct ttccggcgaa tcgcccaccg
      541 cgaaagaggt ccacaggtcc ggcgaggagc gggaatacac gatcgttttt gagtttcacc
      601 gctagaaccg cgctttctga aatagacggt actacccggt agtatgtttc cattggtttt
      661 agtacgaatc gcgcgagagg cactttcttt gagcgattgg atgattccac tgtttgtggt
      721 tcgggtacgc ggaagatgag tgctgtgccg cccgtttcta tgtccgtttg tccgccggtc
      781 tcttctgctt ctgcaggctg tgattccgtt gcatctacat ttcctgtggc tcgatcttcg
      841 agtttgtagg actgagcggt ggatttttca atcactctga gtcgaatggg tcgcacacga
      901 ggacgttcta gacctcgctc tgtatttgcc gttgaaaaac caagggagac attcttccag
      961 tcttcgccgg tcgattgtac tgcgtttcca aacatttcta tttccactga tccatctttt
     1021 gcgagaagtg ctccgtacga aatgctccac gatgcgccgc gaatgttgta acctacttgt
     1081 acttctgcag attgggcgcg gctggacgtt aggcgaactt ccacagtgcg tgcggtcttt
     1141 tcaccctctg ctctggccat atcgagttcc ttctggatgg ctgccagttc cagatcgatc
     1201 ttgcgtccct gttcttcctg tttctgaatc aaagatcgac gtgctgttct ttcgttttgg
     1261 atgaactgag cggctttcgt ccatctcccg ctatccgcag gtcgcatggt ttcttcgccg
     1321 atcacgcggg aagccgcggt ggacagttga ttgacgaagt tcaaaacaaa ctgattgcgc
     1381 tggatggttg cggaaacagt atcccgtttt tcttttacgg ctgcgaactt cttttccagt
     1441 gtctggatgc gtggtcgcac tgtctcagaa aatcggtcca gtcttgaaga tacttccacg
     1501 atgctacaat ccggagcgca ggatgcgcgc aaagtgtccg gcaagagatt ggggctgaca
     1561 ttcgcgatga tcaatctgtt tttgcccgcg acaagtttca cattatcctt tcgcgtgatt
     1621 tgagcgcgat ccgggtaaag aagcacggaa cgcactggca gatcttctgc ggcaagagcc
     1681 gccgtactga gaatgaagat ggttgtgacg attcgaagca ttgctgtgga atgtttcata
     1741 gtgcaggccc cgcttccact ccggaaggag tgatttccgc cgaatggcga agagtgtatt
     1801 tgagtgtaac gatctgttcg gcgccggggc caatgttcag acgaaatcgg gccaaccctt
     1861 ccggcgttcg ttctacagga gccggtgtga gtccagtttc ttctatctta atgcggtcat
     1921 cttccgatct gggaacccgg tcgaatacgt ccagtacgat catttcgcgc ctggagttct
     1981 tgatcttgat ttgaacttcg attttgtagc gcagatctcc gcccagaagt cccgcacttt
     2041 cgcgaaatcg attctctgtg cgagtcacct tgatgtcttc tgtgctaccc agggacagag
     2101 caatcttctg gttgtttcca gtttcaggga gtaccgcctc tcctgtgaag tcgtctccgt
     2161 agaaaatctg tgcggggccc ggtaggagag gtttgtcgtt tttgtactgc acatgcccgg
     2221 tcagatagcc aaacgcctgg tgcatgggtg cgcattcata gactctttct gcctgcaaat
     2281 cagaatcgaa cagagacact ttgcgtagga tgccgtctga cggtatcgtt tcagaatatt
     2341 gagtctcgta tatagcggct ccgcgaagag cgcgcggtgc aattagattg ttcgtgcgat
     2401 tttgaagcgc cgtcaggctc ttctcacgaa tctcgcgaga tttcttttcc tctgttgcga
     2461 agtatttctg gttttcgttt cgcagctttc caagctttcc catgaactct tcgctgtatt
     2521 tggccgcgcc gtcgtagtct ccgtcttgaa atgcgcggtc acgcgaattg agcacggaaa
     2581 gcagggatgc atcttttcca gaagtgattc cctgggattc tttgagggta ttgcgcgagt
     2641 tgctgactgt tttgtttagc tcatcgttta caggcttgtt atccgcgggt agctggctga
     2701 ctacctgatt tgtctgagtt tgaggagcat aagatggttt tccgggtgcc tgagcttctt
     2761 cgtccatcga ttgtctttgc atttcgcgtg caggagcggc cggcgagata ttctgcctgc
     2821 gtttgtctga cgtggcatcg tttatgtttt gttctgcaat catcgctgcg cgaatgcgcc
     2881 attccctgag ctcaggaaga ctgatgaggt cttccgggtt agaagcggac agctgcagtt
     2941 ttacattgtc ccattgttcg cccgtgcggt tttgaaccag cgcgaacgct gaaagtttca
     3001 ctgcttcttt ttctgcacgc cccacacgaa ccgaatatac aggataccag tccgcgcccg
     3061 gtgttctata agatagatta aaatccgctt ctc
//
LOCUS       DAORFF010000484         3084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_20947_length_3084_cov_3.478706, whole genome shotgun sequence.
ACCESSION   DAORFF010000484 DAORFF010000000
VERSION     DAORFF010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20947_length_3084_cov_3.478706"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PKK76_16085"
     CDS             <1..205
                     /locus_tag="PKK76_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70051.1"
                     /translation="LLARVLDTFQPYRKRREELLASPEEVSALMRKGAEKARAAAMPI
                     ISKAREAVGIAPKTATLPKQKKK"
     gene            241..2148
                     /locus_tag="PKK76_16090"
     CDS             241..2148
                     /locus_tag="PKK76_16090"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNL70052.1"
                     /translation="MGRSATVSKAILVLFALCVLLLRSSFDSLATDLYAHAYAWQVAH
                     SDVVPGPRPLPEFAWVFAICTLPLVILLPERIRSWAVASVLFASFCIRLAGIPALPSL
                     PSYSRDPSAAHSNFRREALRVTMVVKESVSSSSHVAEFRITGREVKVRRQWFSISDLA
                     EEEKSAFPVLVHFEGARLYGGCKVHARISLRAMPREIKNESYASYVHRLGAVGTLRLE
                     PWQVLAIECGSPGWRDSVTLFIERSLGHRFRGRTLDASLGFVLGQAGTLDSELKTSAR
                     ELGILHVFAASGMHLAIIYGVIFLPASFLLGRRHPISALLPIGPCFLYLWLLGFPVSL
                     ARAFAFAVVYALTCLFHRRLTSTTLLLNSAILLLFWMPEDFATLSSALSFGAVAGIFY
                     FASPLLTLFRLKGPSAWIWGQVSVSACAGIFTTPLLIFVFGGFSFSSLPANLVAVPLS
                     DLALPVLFGGLGLESMGAPEQVLEIAWKPAQWIMSAFIFLTERAGPHSLYNDFDSIWS
                     LPLFAALGVALTAGYILIRPVECGRRLRVIRALSLVSLLALSPVGGWLGRLFDSSSAR
                     GFIAGFFGVAHRPASYISNHGPDGERGEGNSRSSSGVAQQEARPEFPGGAVSNSDDCP
                     GNPCSSQPDHH"
     gene            2102..2878
                     /locus_tag="PKK76_16095"
     CDS             2102..2878
                     /locus_tag="PKK76_16095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HNL70053.1"
                     /translation="MIAQAILAHPNRTIIEIGPGLGALTRPLVESGRSIIALELDRAF
                     CRYLSEYFERSPNFRLIECDALAKLEEWSRAADWSNVAGPTREAPFVCGNLPYSITTD
                     LLRGVVGVPWLPGGIFLTQLEFAQRITSGSSISSFAIYLGCFGKWSKLRNVGRQFFFP
                     APSVDSALISFERTEMRCNPSTLEDILRSSFHSKRKKISNSWKSAASLRGLREEWLLE
                     LAPAAGLDPGLRPEEVPPEKFYELARLVDQQFGAQIRAQA"
     gene            complement(2869..>3084)
                     /locus_tag="PKK76_16100"
     CDS             complement(2869..>3084)
                     /locus_tag="PKK76_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNL70054.1"
                     /translation="GDQVYISCRILKTGRSSITVETIAEIHAPVTGSHEETIRCKVTF
                     VCLTDNKPFAYFESEDYKKFRQQKTQA"
BASE COUNT          588 a          764 c          851 g          881 t
ORIGIN      
        1 actgcttgcg cgcgtgctcg atacgtttca gccatatcgc aagcggcgcg aagagctcct
       61 ggcttccccc gaagaagtgt ccgcattgat gcgaaaaggg gcagagaaag caagagccgc
      121 ggccatgccc atcatttcga aggcaaggga agcagttgga attgcaccga agactgccac
      181 tctgccaaag caaaagaaga agtaagcgga acgaatgaaa ctgcccgcac actcatctgt
      241 gtgggcagat ctgcaaccgt ttcaaaagca attctggttc tattcgcact ctgtgttttg
      301 cttttgcgct cttcctttga ttcgctggca acggatctct atgcgcatgc atatgcatgg
      361 caggtagcgc attcagacgt tgttccaggt ccccggccat tgccagagtt tgcctgggta
      421 tttgcgatct gcacactgcc tttagtcatt ttgcttccgg aaaggattcg gtcctgggcg
      481 gtcgcctctg ttttgtttgc ttctttctgt attcgtctgg caggaattcc ggctcttccg
      541 tctctgcctt cctatagcag agatccttca gcagcgcatt ccaatttcag gagagaggct
      601 ttgcgcgtca ccatggttgt gaaagaaagc gtttcttctt cttcgcatgt ggcagaattt
      661 cgcataacag gccgtgaggt gaaagtgaga cgtcagtggt tttccatatc agatctggca
      721 gaagaggaga aaagtgcgtt tccagttctt gttcattttg aaggagcaag gctctacggg
      781 ggatgcaagg tgcacgctcg catctcgctc cgtgctatgc cccgtgaaat caaaaatgaa
      841 tcgtacgcat cgtacgtgca tcgactgggc gcagtgggca ccttgcgttt ggaaccctgg
      901 caggtgctgg ccatagagtg tggatctccg ggatggcgcg attctgtgac gcttttcatt
      961 gaaaggtctt taggtcatcg ctttcgcggg cgaacgcttg acgcttccct gggatttgtg
     1021 cttggccagg cggggactct ggattctgaa ctcaaaacaa gtgcgcgtga actcgggatt
     1081 ctgcacgtgt ttgcggcaag tggcatgcat ctggcgatca tctacggagt gatctttctg
     1141 ccggcctcgt tccttcttgg tcgcaggcat cccatttctg cgcttcttcc gattggtcca
     1201 tgtttcctct atttgtggct gctcgggttt cctgtgtctc ttgcaagagc cttcgcattc
     1261 gcagtcgtat acgctctcac ctgtctgttt catcgtcgct taacgtctac tactctgctt
     1321 ttgaattcgg ccatattact cctgttctgg atgcctgagg attttgcgac tctgtctagc
     1381 gctctgtctt ttggtgcggt ggccggtatc ttttactttg cttcgccgct tctaacgctc
     1441 tttcgactca aagggccctc tgcgtggatc tggggccagg tgtctgtgag tgcatgcgct
     1501 ggaatcttca cgactcctct tttgatcttt gtgtttggcg ggttttcatt ttcttctttg
     1561 ccggccaatc tagtggccgt gccattgagc gatcttgctt tgcctgttct gtttggaggc
     1621 ctgggtcttg agagcatggg cgcaccagag caggtgctgg agattgcgtg gaagccggct
     1681 cagtggatca tgagtgcttt tattttttta acggagaggg cagggccgca ttctttgtac
     1741 aatgactttg attccatctg gtctctgcca ctcttcgcgg ccctgggagt tgcgctgact
     1801 gccgggtata ttttgattcg cccggtggaa tgtggccgcc gactgagagt gattcgagca
     1861 ctcagtctgg tcagtctgct ggccctttcg cctgtcggcg gctggctggg tagattgttt
     1921 gattcatcga gtgcgcgggg tttcatcgcg ggcttcttcg gggttgcgca cagacccgca
     1981 tcctatattt cgaaccatgg acctgacggc gaaagaggtg agggaaattc tcggtcatct
     2041 tcaggcgtcg cccagcaaga agcgaggcca gaatttcctg gtggagccgt ctcaaactca
     2101 gatgattgcc caggcaatcc ttgctcatcc caaccggacc atcattgaga ttggcccggg
     2161 cctgggtgcg ctgactagac ctctggtaga atcaggacgt tccatcatcg ctctggaact
     2221 ggatcgtgca ttctgtagat atcttagcga atactttgaa agatcgccta acttccgcct
     2281 gatcgaatgc gacgcgcttg cgaaactgga agaatggtcg cgcgcagcag attggtcgaa
     2341 cgtggcgggt ccaacgcgcg aagccccttt tgtttgcgga aatcttccgt actcgatcac
     2401 aacggatttg ttgcgaggag ttgtgggagt tccctggttg cccggcggaa ttttcttgac
     2461 ccagcttgaa tttgctcagc gcataacgtc tggttcgagc atcagttcct ttgcaatata
     2521 tctgggatgt tttggaaaat ggagtaagct cagaaatgtg ggcagacagt ttttctttcc
     2581 cgcaccttcc gtagattctg ctctcatttc ttttgaaaga acggaaatga gatgcaatcc
     2641 ttctacgctg gaggatatac ttcgctcttc ctttcattca aagaggaaga agatttccaa
     2701 ttcgtggaag tctgcagcca gtctccgtgg tcttcgggaa gagtggctgc tggaacttgc
     2761 gccggccgca gggctcgatc ccgggcttcg cccggaagaa gttccgccgg agaaattcta
     2821 tgagctcgcg cgccttgtgg atcaacaatt tggagcgcag attcgggctc aggcctgagt
     2881 cttttgttgt ctgaattttt tgtagtcttc actttcaaaa taagcaaacg gcttgttgtc
     2941 cgtgagacag acgaaagtta ccttgcagcg gatggtttct tcgtggctgc ctgtgaccgg
     3001 tgcgtggatt tcggcaatcg tttccactgt gatggaagat cttcctgttt ttagaatgcg
     3061 acatgagata taaacctggt ctcc
//
LOCUS       DAORFF010000485         3070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_21129_length_3070_cov_3.228192, whole genome shotgun sequence.
ACCESSION   DAORFF010000485 DAORFF010000000
VERSION     DAORFF010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21129_length_3070_cov_3.228192"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PKK76_16105"
     CDS             <1..296
                     /locus_tag="PKK76_16105"
                     /inference="COORDINATES: protein motif:HMM:NF021631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNL70055.1"
                     /translation="IFSCMWVEERQTVPPMEFDVLRAAVVKDAVLTEIIEDLLTKKKS
                     GTELGLAPQIPELNAFLEERIAHFESVSAEFNPAQKPDAELLNNSFQSILGLR"
     gene            315..698
                     /locus_tag="PKK76_16110"
     CDS             315..698
                     /locus_tag="PKK76_16110"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNL70056.1"
                     /translation="MPEFMLYIRNAKDAKKSLTEDQHLAFIKQCETYIGTLKSKNALL
                     AAQPIVREGVVLKKTEGGWNEKDIAADQETQVGYYHIRAASLEEAIQIAKENPEFDYV
                     PSATIEVRPVKTKEMETGFVYPTNS"
     gene            755..1180
                     /locus_tag="PKK76_16115"
     CDS             755..1180
                     /locus_tag="PKK76_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNL70057.1"
                     /translation="MHNVGIVVESLDNAISFFTELGLTLEGRAMIEGEWAGRVTGLPS
                     QQVEIAMMVTPDGHNRLELSRFLKPAVIADHRNAPVNSLGYLRVMFTVDNLDNTLTKL
                     EKIGSTLVGEVVDYENMYRLCYVRGPEGILIGLAEELQK"
     gene            1331..1741
                     /locus_tag="PKK76_16120"
     CDS             1331..1741
                     /locus_tag="PKK76_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70058.1"
                     /translation="MRFFSIGLVLMFAHCATTLALRERWNGETVTNWEYNPPIQEKIP
                     IIYGGTRMDLRLLTAWKPLLCPVPEDSKTRGRLTHEWAPVAACAGTIDILPSFLLDTV
                     LLPVTIIWSLFEALTENAKTPATEYSRPENQSRD"
     gene            1861..2709
                     /locus_tag="PKK76_16125"
     CDS             1861..2709
                     /locus_tag="PKK76_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70059.1"
                     /translation="MKVTRWTICLIGFCSFCSSLSPGGPPAEITAITYNTHYMQRGVP
                     GLIRTLSPLQADVIAMQEVLVQNGHVSSQTIAQHLGMNHVSSLPYVAYGNSQWVLTIL
                     TRYPVLYVDQRLLGANRRALRVLLNVKGHRVNFVTMHLTPYTNEHPLKEANQIRSDLR
                     KREIKDLLEWIGTPPDPVVLLGDFNMLRGAVRLWGLNEYSLVTSAGYTDADGGFLPTN
                     SDTFPLDEEAKHRAGEKLPEFLVPSGITLDYVFLAGKISVLSTDVLKSDASDHYPLLA
                     RLRVGY"
     gene            2710..>3070
                     /locus_tag="PKK76_16130"
     CDS             2710..>3070
                     /locus_tag="PKK76_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70060.1"
                     /translation="MLRYAAMILQKQEELSHMNKSRIAVIALVALIILCGAVRPDRLI
                     ALAAGAVSQNVCSGTFVSGLSPETVYDQEMRAEGGMSLINWALVYQVDQSERTVTTTV
                     FGRFKTRSRFHEGSGCRI"
BASE COUNT          806 a          781 c          750 g          733 t
ORIGIN      
        1 ccatcttctc gtgtatgtgg gtagaagaac ggcaaaccgt tcctcccatg gagttcgacg
       61 ttttgcgcgc cgctgtagtg aaggacgcag tgctcacgga aatcattgaa gatctgctca
      121 cgaaaaagaa gagcggaacc gaactgggcc tcgctcctca aatcccggag ctaaacgcct
      181 ttctggaaga gcgcattgct cattttgagt cagtctccgc ggaattcaat cctgcccaaa
      241 aaccagacgc agaactttta aacaatagct ttcagagcat cctgggctta cggtagcaaa
      301 gaaggaacaa atcaatgccc gaattcatgc tctatattcg aaacgcaaaa gacgcaaaaa
      361 agtctcttac agaagaccag caccttgcgt tcatcaaaca atgcgaaacg tatatcggca
      421 cgttgaagtc aaagaatgcg ctgctagccg cacagcctat tgtgcgcgag ggagttgttc
      481 taaaaaaaac ggagggtggc tggaacgaga aagacatcgc agccgatcaa gaaactcagg
      541 tgggctacta tcacattcgc gccgcaagtc tggaggaagc catccaaatt gcgaaagaaa
      601 atcctgagtt tgactacgtc ccttctgcta caattgaagt aagacctgtg aagacaaaag
      661 aaatggaaac aggatttgtc tatccaacaa acagttgata ggtgctactc ctgacttgag
      721 aatggcaaac atgaaagaaa ggttgctccc gcgcatgcac aatgttggca ttgtagtcga
      781 atctctagat aatgccattt ccttttttac cgaacttggt ctcacactcg aaggcagagc
      841 catgatcgaa ggagaatggg ccgggcgagt gacggggctt ccttcgcagc aagtcgaaat
      901 tgcaatgatg gtcactcccg atggccacaa tcgcctggaa ctctccagat ttctgaaacc
      961 agcggtgatc gcagaccata gaaatgctcc ggtaaactct ctgggttatc tgcgcgtgat
     1021 gttcacagtc gacaacctgg acaacactct gacaaaactc gaaaagattg ggtctactct
     1081 ggtgggagaa gtagttgatt acgaaaacat gtatcggctg tgctatgtcc gtggccctga
     1141 gggaatcctg attggactgg cggaagaact ccagaaataa gaaagcaacg ttaagcgaaa
     1201 atacgatacc gacttgacag aacaaaaagg tgcgcggcgg caagatgtat ggtaacttta
     1261 cagtccggcc tctcttcaaa agtatgaagc cagaggagct cgcgcagttg aaagcacttc
     1321 tagcagagcc gtgagattct tttcgattgg actcgtactg atgttcgcgc attgcgctac
     1381 tactctcgct ttgagagaac gctggaacgg agagactgtc accaactggg aatacaatcc
     1441 tcctatccag gagaagattc ctattatcta cggaggtact cgcatggatc ttcgcctgct
     1501 tacagcatgg aaacctcttt tatgcccggt ccccgaagat tctaaaactc gcggtcgact
     1561 cacgcacgaa tgggctcccg tcgccgcctg tgcgggaaca atcgacatac tgcccagctt
     1621 tcttctggat actgtgcttt tgcctgttac cataatctgg agcctgttcg aagctctaac
     1681 tgagaacgcg aaaactcctg ctaccgaata ctcaagaccg gaaaaccaat ccagggattg
     1741 accatgccat ttgcaccccg cttgcgaaag acatccttgt ttttctggtc acgtaattct
     1801 tctcatcgga ggagctcttt ctgtttgcgt cttctacctg gcgaccgatt ttggacctcg
     1861 atgaaagtaa ccagatggac catctgcttg attggctttt gctctttctg ctcatctttg
     1921 tctccgggtg gcccccccgc ggaaatcacg gccattactt acaatacgca ctacatgcaa
     1981 agaggcgtgc cggggctcat tcggactctt tcgcctttgc aagcagatgt catcgcaatg
     2041 caggaagtgc tcgtgcaaaa tgggcatgtt tcatcgcaga ccattgctca acatcttgga
     2101 atgaatcatg ttagcagtct gccatacgta gcttacggga acagccagtg ggttctgaca
     2161 attctaacgc gctaccccgt tctctatgtc gaccagagac tgctcggtgc aaatcgccgc
     2221 gcattacgag tgcttcttaa tgtgaaaggc cacagagtga acttcgtgac catgcacctc
     2281 acgccgtata ccaatgaaca tccactcaaa gaagcaaatc aaataagatc cgatttgcga
     2341 aagcgcgaaa tcaaagatct gctcgagtgg atcggaaccc ctcccgatcc agttgtattg
     2401 ctcggcgatt tcaacatgct tcgaggtgcc gtgcgactgt gggggctaaa cgagtattcg
     2461 cttgttactt ccgcaggcta cacggatgcg gacggcggct tccttcctac gaactcagac
     2521 acgtttcccc tggacgagga agcaaagcat cgcgccggcg aaaagcttcc ggaattcctc
     2581 gtgccctctg gcatcaccct cgactatgta ttcctggctg gtaaaatctc cgtcctttct
     2641 acagatgtac tgaaatcgga cgcatctgat cactatcctc tccttgccag gctgcgagtc
     2701 ggttactagt tgctacgtta tgcggcaatg attctacaaa agcaggaaga gttgagtcac
     2761 atgaataaat ccagaatcgc agtcatcgca ttggtcgcgt tgatcatcct ttgtggagcg
     2821 gtacgaccag atcgtttgat tgcacttgcg gcaggtgcag tcagccagaa tgtatgctcg
     2881 ggcacttttg tttcagggct ctcacccgaa acagtctatg atcaggaaat gcgcgcggaa
     2941 ggcggcatga gtctgatcaa ctgggcgctt gtttatcaag tggatcaatc cgagcgcaca
     3001 gtgactacca ctgtgtttgg ccgattcaag acaaggtctc gcttccatga aggatctggt
     3061 tgccgcatcg
//
LOCUS       DAORFF010000486         3064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_21203_length_3064_cov_2.653041, whole genome shotgun sequence.
ACCESSION   DAORFF010000486 DAORFF010000000
VERSION     DAORFF010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21203_length_3064_cov_2.653041"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PKK76_16135"
     CDS             <1..706
                     /locus_tag="PKK76_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70061.1"
                     /translation="GAGPQMSATFGIVCGIALNFAVIMGAANLLYTVSRGPSRVWSNL
                     DGSYLVWGVRFILILAILRAISLPRFMQADVGYTWWNSRDISQDAIIAASVVFAGLAY
                     GYSQRFNQKAAANTLASAALLLAVVGSCLMIVAGTVQGILGGMSARAMTEAGPAVTSF
                     SALLFAGNVAGDTASPTLKFILGLRGIYFLGYASVTVSLATWALSMLSIWRGQGNPFQ
                     EADLHLPDEENPEEAR"
     gene            706..1362
                     /locus_tag="PKK76_16140"
     CDS             706..1362
                     /locus_tag="PKK76_16140"
                     /inference="COORDINATES: protein motif:HMM:NF014486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HNL70062.1"
                     /translation="MSRIYEKLFSSPLSTVLSSIAIVIVGYGIAVCLPFSENISHGSL
                     SQLSTEDTLVLKGHEVYYQEGCQYCHSQNLRPFGWEVSRYVNVEKYGYYPVPTAMEYS
                     HETPAMRGSFRIGPDLSRVAGKQDAAGLTSLLRGGKEDNLRNRYHAYGYLFNDDDLQP
                     LFLSWKIRMMLEAGVPLSDPYHKSAFDRLEGQTRGDALVAYLLSLGQKSMQYNGAFYR
                     "
     gene            1378..1899
                     /locus_tag="PKK76_16145"
     CDS             1378..1899
                     /locus_tag="PKK76_16145"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNL70063.1"
                     /translation="MSEHSSTSQQKGPSKHGLSWIYFAIAFTFVAGTAYAIFYHGFLG
                     ATNATTYRESVGMSVVAVTEDFIPERTPQAIANGAASYKTCAACHGANLEGLACPNLA
                     DNEWLHFKEPKETNLAKLIKGGVPAGATKGPKGGAMPPMGTLDNMTKVWEVIYYLQTK
                     NPNIIKDSVPTKK"
     gene            complement(1933..>3064)
                     /locus_tag="PKK76_16150"
     CDS             complement(1933..>3064)
                     /locus_tag="PKK76_16150"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNL70064.1"
                     /translation="EKPVVTIGSLLLSRIVETRPRLLDFLMSLSDLVSVGMITDMVPL
                     AGENRSLVRIGLGMAGKRVRRGVPGYRALIGALDLPEFDLTGRDLAWSIGPAINAAGR
                     MGNTALALDLLTSDSPVEAERLARELKALNKKRQERTRSNERIVTEHFEAYPEKTDRP
                     IIFCYHESLEPGVSGIMATRLLEKYGKPVVYINPDGKWAKGSARGEGHNVLQMLDCAA
                     HLFIQFGGHPEAAGFSLPYERIEELERVLLENAPFMDLEPVSTELRPHLDLLPEQINR
                     RLYDELRQMEPFGPGNPEPLIRITGAQVTDVTYMKDKLHARFRIRGSRDIEFVAWRRG
                     TEFGKDMERVDLIGSLERSIFAGRTRIQFRVEHVKAAAVLVA"
BASE COUNT          729 a          838 c          769 g          728 t
ORIGIN      
        1 tggcgcgggc ccgcaaatgt cagcgacgtt tggaattgta tgcggaattg cactgaactt
       61 tgcagtgatc atgggagccg ccaacctgct ctatacagtt tcacgcggac caagcagagt
      121 ctggtccaat ctggacggat cctatcttgt atggggtgtg cgattcattt tgattctcgc
      181 catccttcga gccatttctc ttccgcgctt catgcaagcg gatgtaggat acacctggtg
      241 gaactcccgc gatatttcac aggatgcgat cattgctgca tctgtggtat tcgctggcct
      301 ggcgtatggc tattcacaga gattcaatca gaaggccgcg gcaaacacac tggccagtgc
      361 cgcactactt cttgcagtag taggaagctg cctgatgatc gtggcaggca cagtgcaggg
      421 aatcctgggc ggcatgtctg cccgcgcgat gacagaagcc ggcccggcag tgacttcctt
      481 ttctgcactc ctgtttgcag gcaatgttgc aggagacact gcatccccca ctttgaaatt
      541 cattcttgga ctccgtggaa tctacttcct gggttacgca tccgtcacag tgagcctcgc
      601 cacctgggcg ctcagcatgc tgtcgatctg gagaggccag ggaaacccat tccaggaagc
      661 agatcttcac cttccggatg aagaaaatcc agaggaggca cgctgatgtc ccgcatttac
      721 gaaaagcttt tctccagtcc actgagcact gtactatctt cgatcgccat tgtgatcgtt
      781 gggtatggga tcgcagtgtg cctgcctttc agcgaaaaca tttctcatgg ctctctctcg
      841 caattgagca cagaagacac tctggttctc aaaggccatg aagtgtacta ccaggaaggc
      901 tgccagtatt gccattctca gaatctcaga ccatttggct gggaagtgtc acgctatgtg
      961 aacgttgaaa agtacggcta ctatccggtt ccaactgcaa tggaatattc gcatgaaaca
     1021 cctgctatgc gaggaagttt cagaattggt ccagatcttt cgcgtgttgc tggcaaacag
     1081 gatgcggctg gcctgacttc cctgctacgc ggtgggaaag aggacaacct gcgcaatcgc
     1141 tatcatgctt acggctatct gttcaacgac gatgatctcc agccgctgtt tctttcgtgg
     1201 aagattcgca tgatgctcga agcgggagta ccgctttcag atccatatca caagagtgct
     1261 ttcgatcgcc tggaaggaca aacacgcgga gacgcgcttg tggcctatct tctgagtctt
     1321 ggacagaaga gcatgcaata caacggcgca ttttatcgct gaggctaagg tataattatg
     1381 agcgaacatt catcaacctc gcagcaaaag ggaccgagca aacacggtct atcctggatt
     1441 tactttgcga ttgcatttac ttttgttgca gggacagcat acgcgatctt ctatcacggc
     1501 ttccttggcg cgacgaacgc aaccacatac cgcgagtctg tgggaatgtc cgttgttgcc
     1561 gtgacagaag atttcattcc tgagcgcact ccgcaggcga tcgcgaatgg agcggcgtct
     1621 tataaaacgt gcgccgcctg ccacggtgca aacctcgaag gtcttgcctg tccaaacctg
     1681 gccgacaacg aatggcttca cttcaaagag ccaaaggaaa ccaacctggc caaactgatc
     1741 aaaggcggcg ttcctgcagg agcaaccaaa ggcccgaagg gtggcgcgat gcctcctatg
     1801 gggactctgg acaacatgac caaggtgtgg gaagtcatct actacctgca gaccaagaac
     1861 ccgaacatca tcaaagactc agttcctacc aagaagtaaa tttcaacgtc aggcggcatt
     1921 ggcgccgcct gatcacgcca ccagaactgc ggccgctttc acgtgttcca ctctgaactg
     1981 aatccgcgtt ctgcctgcaa aaatgctccg ctccagtgaa ccaataagat ccaccctttc
     2041 catatccttg ccgaactccg tcccgcgacg ccacgctaca aattcgatgt cccgacttcc
     2101 ccggatccga aagcgcgcat gcagtttgtc tttcatgtaa gttacgtctg tcacctgcgc
     2161 acccgttatg cgaatgagtg gttccgggtt gccggggccg aatggctcca tctgtctgag
     2221 ctcatcataa agacgacggt tgatttgttc gggcaataga tccagatgcg gcctgagttc
     2281 ggtgctgacg ggttcgagat ccatgaaagg tgcgttctcc agtagaactc tttccagttc
     2341 ttcaattctt tcatagggaa gagaaaaacc ggccgcttcc ggatgaccgc caaactgtat
     2401 gaacaaatgc gccgcgcaat caagcatctg gagcacatta tgcccttccc ctctggcaga
     2461 ccctttggcc cattttccgt ccgggttgat gtagactaca ggcttgccgt acttttcaag
     2521 aagccttgtg gccataattc cagaaacacc cggctccagg ctttcatgat agcagaatat
     2581 aatgggacga tctgtcttct ctggatacgc ttcgaaatgt tccgtcacga tgcgctcgtt
     2641 ggacctggtg cgctcctgtc tttttttgtt gagtgctttg agctcacgcg cgaggcgctc
     2701 cgcttcgacc ggagagtcgc tggtgagtaa atccagtgca agtgccgtgt ttcccatcct
     2761 gcccgcagca ttgatggcag gaccaatgga ccaggccagg tcccgacctg tcagatcaaa
     2821 ctctggcaaa tcaagagcac caatgagcgc tctgtaacca ggaactccgc gcctcactct
     2881 ttttcctgcc attccaagac cgattcgcac aagagatctg ttttccccgg ccagaggaac
     2941 catatccgtg atcattccaa cagaaacaag atcagataga gacatcaaaa aatcaagaag
     3001 gcgcggacgg gtttccacaa tgcgcgacaa aagcaaagaa ccaatagtaa cgactggttt
     3061 ttca
//
LOCUS       DAORFF010000487         3045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_21442_length_3045_cov_3.067224, whole genome shotgun sequence.
ACCESSION   DAORFF010000487 DAORFF010000000
VERSION     DAORFF010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21442_length_3045_cov_3.067224"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PKK76_16155"
     CDS             complement(<1..881)
                     /locus_tag="PKK76_16155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNL70065.1"
                     /translation="MKLERELAIRINPEKSRRFTAEQFAANLARLTDAALSMQGEDRT
                     IVSVVGTNGKGSTSYYLAQLFESQTGLYTSPHLHSVYERIRVNLESISETECDAALLR
                     LEKQHDLSNYSYFELLTILAGFLFHEKNIPVQIFEAGLGGRLDATRIFRAQYVVLTSI
                     GLDHTELLGNSHEEILAEKLGICTKNARFVLCANQPHLSRKTVEEMSPVPVLFFDEPY
                     LPNSYLAFNQRFAIFCAKKIAEDSSRTLSSISSPLRDPPGRLERRNLNQRAVFFDNAH
                     NPDAIHTALSSLRGFPD"
     gene            complement(878..1771)
                     /locus_tag="PKK76_16160"
     CDS             complement(878..1771)
                     /locus_tag="PKK76_16160"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNL70066.1"
                     /translation="MPEHPVAASGKTRVYGILGNPVAHSLSPLIHNAAFQFKKIDAAY
                     VPFHTTQTGAFLKKAVVSMGIRGLSITIPHKIWAAKAADQKDPLTKLCGAANTWIIEN
                     GIVSAHNTDGPGAVRAMQEKHKGVGKRYLILGYGGTALAIAHALLLESSPRSITITGR
                     NLKKAARFAEDLRQAHARHRSLIDSAPLSQIAPEQIDAVIQTTPSGMNAEKGSKVLPE
                     LGFDPDWITKNHLVFDVIYVPMRTPLIELAERRGSTIVYGYKMLLYQAVRQFELFTGQ
                     SAPESLMERLLLSHLRNGTRR"
     gene            1884..2207
                     /locus_tag="PKK76_16165"
     CDS             1884..2207
                     /locus_tag="PKK76_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70067.1"
                     /translation="MSFLDLILQLVVYTASGIAASYYFYNWKRKDLLGGFWGGTVIGL
                     VGAVLISWVTSRDAWFIRLVSWLMIPKFGDGFQIPVNLIASVLGGLLFVAILNRINHN
                     KDRRF"
     gene            2297..>3045
                     /locus_tag="PKK76_16170"
     CDS             2297..>3045
                     /locus_tag="PKK76_16170"
                     /inference="COORDINATES: protein motif:HMM:NF014518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HNL70068.1"
                     /translation="MPSPPMPGLGTGADTQKVIQQLIEVERRPLKRIILDNESSGQSI
                     KIWEEARRRSRNLQDKSQVLYSFSGAFATKAIVSSDPGAVTGQASPDMDDQSMQIEVS
                     KLATHHRIHSAPVSAEKDLPAGTFTIKVGEKSLEYDFPGGKLTALVRLIKSKAAGTID
                     VNMIRKDSETSIVVLRSLTSGTKGKMEFVDAGVLATLGLPPGAGGASEVEAPQDAVLK
                     LHGVEVTRTSNENITDVLEGASLTLRKVTTGP"
BASE COUNT          713 a          734 c          801 g          797 t
ORIGIN      
        1 aaatcgggaa agccgcgcag agaagataga gcggtatgga ttgcatccgg gttgtgtgca
       61 ttgtcaaaga aaactgctct ctggtttaga ttccttctct cgagtctgcc cgggggatca
      121 cgcagaggcg atgagatgct cgacagggta cggctggaat cttccgctat tttttttgca
      181 cagaatatcg caaacctttg attgaatgcg aggtaagaat ttggaagata cggttcatcg
      241 aagaaaagaa cgggaacagg agacatttct tccacggtct ttctcgaaag atgaggctga
      301 ttggcgcaca gaacgaacct cgcgtttttc gtgcagattc ccagtttttc tgcgagtatt
      361 tcttcgtgtg aattgccaag caattctgta tgatccaggc ctatggaagt aagtactacg
      421 tattgtgcgc gaaagatcct ggtcgcatcg agtctaccgc ccagacccgc ttcaaaaatc
      481 tgaaccggaa tgttcttttc gtggaagagg aagcctgcga gaatcgtaag caattcgaag
      541 tagctgtaat tggaaagatc gtgctgcttt tcgagacgca atagtgccgc atcgcattct
      601 gtttctgata tgctctccag gttgactcga attctttcgt aaacagaatg aaggtgaggg
      661 ctggtataaa gacccgtctg gctctcaaac agttgcgcca gataatagga agtggatcct
      721 ttcccgttcg ttcctaccac agacacaatc gttctatctt cgccctgcat ggatagagcg
      781 gcgtctgtca accgtgcaag gtttgctgca aactgttctg ctgtgaatcg cctgcttttt
      841 tctggattga ttcggatggc gagctcgcgt tcgagcttca tctgcgagtc ccgtttcgaa
      901 ggtgagagag gagcagtcgt tccatcaatg actcgggcgc agattgtccc gtgaacagtt
      961 caaactgtct aacagcctgg tagagtagca ttttgtatcc ataaacaatt gtggatcctc
     1021 ttctttctgc cagttcgatc aatggtgtgc gcatgggaac atagatgaca tcaaatacta
     1081 aatgattttt agtaatccag tccggatcaa aaccgagttc cggaagaacc ttacttcctt
     1141 tttccgcatt cattcccgac ggagtcgtct ggatcacagc gtcgatttgt tcaggagcga
     1201 tctgtgagag tggtgcagaa tcgatcaagg agcgatgacg cgcgtgcgct tgtcttaagt
     1261 cttctgcgaa gcgtgccgcc ttttttagat ttcttccggt gatcgtgatg gagcgcggag
     1321 aagattcgag gagcagagca tgggcgatcg caagtgcggt gccgccgtaa ccgaggatga
     1381 gatacctttt gcccacgcct ttgtgttttt cttgcatggc ccgaaccgca ccaggtccat
     1441 ctgtattgtg cgcgcttaca attccatttt cgatgatcca ggtattggcc gccccgcaca
     1501 acttcgtaag aggatctttc tggtccgccg ctttcgcagc ccagattttg tgcgggatgg
     1561 taatggacag cccccgaatt cccatcgaaa ccactgcctt cttgagaaat gctcctgttt
     1621 gcgttgtgtg gaagggcacg tatgctgcat cgatcttctt gaattgaaag gctgcgttgt
     1681 gaatgagcgg gctgagagaa tgcgcgacag gattgcccag gattccgtag accctggttt
     1741 tgccgctggc cgctactggg tgttcaggca ttttttttga gtggagccgc ccgctctgtt
     1801 gtaaagagct atttcccccg caggcatgga taaatcatgg taagctcagc cggcaaaata
     1861 gccgatctgt ttattgttac aacatgtcct ttctagatct gatactccaa cttgtagtct
     1921 acaccgcatc tgggatcgct gcgtcttact atttctataa ttggaaacgc aaagaccttc
     1981 tgggcggctt ctggggtgga actgtcattg gactcgtggg tgccgttctc atcagctggg
     2041 tgaccagccg ggatgcgtgg ttcattcgtc tggtgagctg gcttatgatt cccaaattcg
     2101 gggatggatt tcagattccg gttaacctca tcgcctccgt tctgggcggt cttctctttg
     2161 tggcgattct caacagaatc aatcacaaca aagatcgtcg tttctagctc tcgcatccct
     2221 gcgtgcgggc tatctttgcg ctttctggct attatcattt tttcaaaacg cccgatgata
     2281 gtatcaggta ccaattatgc catcgcctcc catgccagga ctcggaacag gagcggatac
     2341 tcaaaaagta atccagcagc tcattgaagt agaacgcaga ccgctcaagc gcatcattct
     2401 cgacaacgaa tcctcaggcc agtccatcaa gatctgggaa gaagccagga gacgttccag
     2461 aaaccttcag gataaatcgc aggtgctcta tagcttctct ggcgcattcg caaccaaggc
     2521 gattgtatcg agcgatccag gtgcagtcac agggcaggct tcgccagaca tggatgatca
     2581 atccatgcag atagaagtaa gcaagcttgc cactcatcat cgcattcatt ccgcgcctgt
     2641 cagcgcagag aaagatctgc ctgcgggtac cttcactatc aaagtcgggg agaagagtct
     2701 tgagtacgat ttccccggtg gcaagctcac cgctcttgtg cggctcatca agagcaaagc
     2761 cgctggaacg atagacgtga acatgattcg caaggacagc gaaacttcca tcgtggtttt
     2821 gcgcagtctg acgagcggaa ccaaagggaa aatggaattc gtagatgcag gcgtgcttgc
     2881 gactctgggt cttccccctg gagcgggtgg tgcctctgaa gtggaagctc ctcaggatgc
     2941 agtgttgaag ttgcacggag tagaagtgac caggacttct aacgagaaca ttacagatgt
     3001 cctggaaggt gcaagtctca cactgcgcaa agtgactacc gggcc
//
LOCUS       DAORFF010000488         3043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_21471_length_3043_cov_4.028447, whole genome shotgun sequence.
ACCESSION   DAORFF010000488 DAORFF010000000
VERSION     DAORFF010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21471_length_3043_cov_4.028447"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..592)
                     /locus_tag="PKK76_16175"
     CDS             complement(8..592)
                     /locus_tag="PKK76_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70069.1"
                     /translation="MDPFTANKYYAKKKLLKLFGGEVRIYDESRTNLLFFVKQKAFKL
                     KEDITVFADESQSRPLLKIQARSILDFGTTYDVTDVVTNQKVGAIRRKLLKSILRDEW
                     AILDASDKEFGTVSEDSMLMALLRRFLSNLIPQHFAMTVGGTQVGDLKQTWNPFVPQF
                     NVDFSMDSGRLDRRLGIAAVVLLQVIEGKQSSMD"
     gene            complement(596..1396)
                     /locus_tag="PKK76_16180"
     CDS             complement(596..1396)
                     /locus_tag="PKK76_16180"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNL70070.1"
                     /translation="MEISTVLGLVGGFTVVLLTLLIEGGHVASFINFGALILILGGSV
                     AVAVVSFSLKEVTAIPQYFMKAIFPPKENLKEMVELFVNLSERARREGLLSLEDDLSS
                     FKDKMMRTGMELVVDGTDPEIVRDILEDYADAEKEHDKIPPELMETLGGFSPTLGIIG
                     TVMGLVHVLEGLGGGGGIEQLGRGIAVAFIATFYGIGFANLLWLPMANKLKFLSHRSV
                     THRNIIIKGVMSIQSGDHPRVIREKLLCQISDPHLRQEIEEAAPKEGA"
     gene            complement(1410..2144)
                     /locus_tag="PKK76_16185"
     CDS             complement(1410..2144)
                     /locus_tag="PKK76_16185"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNL70071.1"
                     /translation="MARKNKAPEQHKASGESGSGRWMLTYLDMVTLLFGVFVILYAMS
                     QVDTNKMKAVAESLRMGFQGGATIDEGSLSGGRTIIEDLFPEGVRARRVRARITGLFR
                     QEMLQKLIKVTEDERGVVVSLIGMDNFRPGSSELTEETQQLLEKMSLLLAEVEYPVRI
                     EGHTDDEVQDNNGAGISASDWELGAKRSVNVILFLETRDVDPRKMSAVSFGSTRPLDR
                     EGTPESHALNRRIDIIIVTGEKLKVK"
     gene            2315..2839
                     /locus_tag="PKK76_16190"
     CDS             2315..2839
                     /locus_tag="PKK76_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70072.1"
                     /translation="MRRIVWTLVLFTATVSANSSEKRALLLPVQAIFIHESMPARPEA
                     ALGLQIERGASMMIQSRGLRLTGASSATITKPEPGLVQSLAAKHGVDLVYWVEATQKE
                     LNYPRENSAQTAFCDDSEKDEPIRGSLSIKVRIFDAEGKEVWTASSTNDYRCSFPVTE
                     AWDQTMRLLEKSPF"
BASE COUNT          791 a          806 c          691 g          755 t
ORIGIN      
        1 tctttcatca atccatcgag ctttgcttgc cttcaattac ttgcagaaga acgacagccg
       61 caatcccgag cctgcgatcg agcctgccag aatccataga aaaatcgacg ttgaactgag
      121 gtacgaatgg attccaggtt tgcttgagat ctcccacctg agttccgccc actgtcattg
      181 caaaatgttg cggaattaga ttggagagaa aacggcgcag aagagccata agcatgctat
      241 cttctgaaac ggttccgaat tccttgtccg atgcatccag tatggcccac tcatctcgca
      301 gtatagattt aagaagtttt ctgcgaatgg cacctacctt ctggttcgtg accacgtcgg
      361 tcacgtcata ggtcgtgcca aagtccagaa tgctccttgc ctggattttg agaagaggac
      421 gcgattgaga ttcgtctgcg aaaacggtaa tgtcttcctt cagcttgaaa gctttttgtt
      481 tcacaaagaa aagcagattg gttctgcttt catcatagat acgcacttct ccgccaaaca
      541 gcttgagcag tttctttttc gcataatact tgttagctgt aaaaggatcc atgaattacg
      601 ctccctcttt aggcgccgct tcttcaattt cctgacgaag gtgcggatct gaaatttgac
      661 acaatagctt ttcgcgaatt acacgcggat gatccccact ctgaatagac attacacctt
      721 tgatgatgat attgcgatga gtcacggatc tgtgacttaa gaatttcaac ttgttggcca
      781 tggggagcca gagaaggttc gcaaaaccaa tcccgtaaaa cgtagcaatg aacgcaacag
      841 caatgcctcg accgagctgt tcaataccac caccaccgcc aaggccttca agtacgtgca
      901 cgagacccat aaccgttcca atgatcccaa gagttgggga gaaaccaccg agagtttcca
      961 tcaattcagg aggaatctta tcgtgttctt tttccgcatc tgcgtagtct tcaagaatat
     1021 ctcgcacaat ctccgggtca gtaccatcca cgacgagttc cattccagtt cgcatcatct
     1081 tgtctttgaa actggaaaga tcatcttcga gactgagcag accttcgcga cgagctcttt
     1141 cagaaagatt cacaaacaac tcgaccattt ctttcaagtt ttcctttgga ggaaaaattg
     1201 ctttcatgaa gtactgaggt atggctgtta cttctttcaa actgaaggaa accaccgcaa
     1261 ccgcgacaga accaccgaga atcagaatca aggctccgaa gttgatgaat gacgctacgt
     1321 gaccaccctc gatgaggaga gtcaagagaa cgaccgtgaa acctcctacg aggcctaaga
     1381 ctgttgatat ttccatgctc gggactccgc tactttactt tgagcttttc tccagttaca
     1441 atgatgatgt caatgcggcg attgagggcg tgcgattcgg gagttccctc gcggtccaga
     1501 ggacgagtac ttccaaagga aacggcactc atctttcgcg gatccacatc acgcgtttca
     1561 agaaataaaa tcacattcac agatcgctta gcacctaact cccagtcaga cgctgagatt
     1621 cccgctccgt tgttgtcctg gacttcgtcg tctgtatggc cttcaatgcg aaccggatat
     1681 tccacctcag ccagaagaag tgacatcttt tcaagaagct gctgcgtttc ctctgtgagc
     1741 tcggaagatc ctggacgaaa gttatccatt ccgatcagac ttacaaccac tccgcgttcg
     1801 tcttcagtaa ccttgatcaa tttctggagc atctcctggc gaaagagccc cgttattcgc
     1861 gcacggacac gccgcgctct tacaccttcc ggaaaaagat cctcgatgat ggttctgccg
     1921 ccactcagac ttccttcgtc aatggtggcc ccaccctgga accccatgcg aagagattcg
     1981 gccactgcct tcattttgtt tgtatccacc tgggacattg cgtagagaat tacgaataca
     2041 ccgaacagca gggtcaccat gtccaggtat gtgagcatcc atcgcccact gcccgactcg
     2101 ccgcttgctt tatgctgttc cggtgccttg ttcttgcggg ccatgaatta tgtcgcatca
     2161 taggggtctg tacaatcctg tcaaattcaa tcgggcccct ggccccgtca ggaccgcaaa
     2221 aaacagcctg tttgacgcac ttttgcgctt tttcctggtg gaacagtaaa gattggcgca
     2281 aattgagcga tacagagtgt tggtaggata taacatgcgc cgaatcgtct ggaccctcgt
     2341 tctatttaca gccactgtat cagcgaactc ttctgaaaag agagcccttc tgcttccagt
     2401 gcaggccatc ttcattcatg aatccatgcc tgcacgcccg gaggcagcgc tcggtcttca
     2461 gattgagcgc ggtgcatcca tgatgattca gtcacgcgga ctgcgcctca cgggagcatc
     2521 cagcgccacc attacaaaac ctgaaccagg cctggtgcaa agccttgcag ccaaacacgg
     2581 agtggatctg gtttactggg tagaagccac tcaaaaagaa ctgaactacc ccagagaaaa
     2641 ctccgcgcaa actgctttct gcgacgatag cgaaaaagac gaaccaattc gcggatctct
     2701 gagcattaag gtgcgaatct tcgatgcaga aggtaaagag gtctggactg caagcagcac
     2761 gaacgactat cgctgttcat ttccggttac ggaagcgtgg gaccagacca tgcgtttgct
     2821 tgaaaagtct cctttctaac cgaatttcaa aatcgactaa cgttaacagg gcttgagaag
     2881 ctcctccggg aacgttagtc gtcatagatc agacgaatta attgcgaatt ggtagagaca
     2941 acagccccat tggatccagg ggcacccagt caatgaacaa gcttacgtgc aagtgagaac
     3001 cggtagcggc tccagttgca cctgccagac caattagatc tcc
//
LOCUS       DAORFF010000489         3016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_21791_length_3016_cov_3.379601, whole genome shotgun sequence.
ACCESSION   DAORFF010000489 DAORFF010000000
VERSION     DAORFF010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21791_length_3016_cov_3.379601"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2168)
                     /locus_tag="PKK76_16195"
     CDS             complement(<1..2168)
                     /locus_tag="PKK76_16195"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNL70073.1"
                     /translation="MASEKLPPLWARIEPVLRPWTERLKRSWKTILIRTFAICFFALT
                     VLTLGVFVRFLYDRAEISASLEKEKMWLRGQGDRPAKPMIQILANDDSLIGEYLPQRE
                     SRIPLDACTNQMVWLSRAAVSSEDKDFFTHGGVSYRGILRAFINNIRRFSVREGGGSI
                     TQQVARNLYIDRSTPGLIRKIYETFAAFLIESKLSKQEILCLYMNRIYMGEGRLGAEE
                     ASWFYFRKPPTSLDAAEAAMIVGLFPSPVRYSPMNNIKLSLHKQEMVLDAMERDGFIK
                     PGEQPGILKYFKARYEITEKSPGKIGLYGASRDFRLNSAPAANDRVRQFLYENIPEEI
                     VREGGLTVRTTIDPIKQAAALDAVRRGIERVRADMINKARDKTFAETQSRRLNGTLIS
                     MDPHTGELRALVGGYGVVETGSSDRIWKMQRQPGSAIKGFLYASALNAGSIDFEAEVV
                     DEPVSFNGYVPKNSYGSYKGKMTLRQAVALSCNTIAVKTLHELGPGEFRDMLGRALEL
                     SYFDTRERFQSNLTLALGSGEVTPLELARIYGILLNEGTTVRPVLIRSVTDKSGTVLW
                     EGTASSGSRVLSAESSSQAIDLLRGVVDPEDEGTAGWIGQQKLKNPNYLPFPIAGKSG
                     TVQTVPEVLRKFPGLTGVHDAWFVGLVPGDVSVVWIGQDEGAPFPGGGSGSAGTIWAD
                     YANSGFRSVTGDFPPVKRKKPVPEGSDPNSIVPLEPAPPPP"
     gene            complement(2252..>3016)
                     /locus_tag="PKK76_16200"
     CDS             complement(2252..>3016)
                     /locus_tag="PKK76_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70074.1"
                     /translation="KPFVLFLAALTLFTAECKQGGKISQSTETRVTRFRMGVLKTMGD
                     PKSALPLSLEKGEVVTLDRIESYKPAQGAAVEYAKIKIAGGQEGYVVASYLAKSGVVI
                     TGDAKLYQRPNITSGLARGNSNLTVGTVAFVENEEFSDGEWVEVSGGANSASYFRGWL
                     KGGAGISKDTELVTAAVRFEQAAEIYKNPKASSKTKEEATATLKDLASSAPAPINQLA
                     RELASGDLPPDNPPVEPKRDEGIQPLEQPSTTPQSN"
BASE COUNT          635 a          798 c          770 g          813 t
ORIGIN      
        1 ggaggcggcg gggctggctc aagaggcaca atcgaatttg gatcgcttcc ctcaggcact
       61 ggcttctttc gctttaccgg tgggaaatca cccgtcacag atctaaatcc ggagtttgca
      121 tagtctgccc agatcgttcc tgcagaaccc gatccgcctc caggaaacgg cgcaccttcg
      181 tcctggccaa tccacaccac agatacgtct ccaggaacca gccctacaaa ccacgcatcg
      241 tgcacgcctg tgaggcctgg aaatttgcgc agaacttctg gcactgtctg gacagttccc
      301 gacttgcctg caattggaaa tggcaaatag ttgggattct ttagcttctg ttgaccgatc
      361 catcctgcag ttccctcgtc ttcagggtct accactccgc gcaagagatc gattgcctga
      421 gacgatgatt ctgcggacag tactctggat ccggacgatg cggttccttc ccaaagaacc
      481 gttccgcttt tgtcagtcac actgcggatg aggacagggc gaacagtggt tccttcattg
      541 agaagaatcc cgtagattcg cgcgagttcc aggggagtga cttctccgga tcccagggca
      601 agagtgaggt tggattgaaa gcgttcgcgt gtgtcaaagt agctgagctc aagagcccgg
      661 cccagcatgt ctctgaattc tcctggaccc agttcatgaa gcgttttcac cgcaatcgta
      721 ttgcacgaaa gagcaaccgc ctgtctcaga gtcatcttgc ctttgtaaga tccgtatgaa
      781 ttttttggaa catacccatt gaatgatact ggctcatcga caacttctgc ttcaaaatcg
      841 atcgaacccg cgtttagtgc gctcgcgtac aaaaagcctt tgattgcgct tcctggctgg
      901 cgttgcattt tccagattcg atccgagctg cctgtttcca ctacaccgta tccaccaacg
      961 agtgcgcgca gctctcctgt gtgtggatcc atggaaatca aagtgccgtt cagtctgcgt
     1021 gattgtgttt cagcaaatgt cttgtcgcgt gctttgttga tcatatccgc acgcactctt
     1081 tcaatcccgc gtctcactgc atcgagggct gcggcctgtt tgattggatc gatggttgtg
     1141 cgaaccgtca aaccaccttc gcgcacgatc tcttccggaa tattttcgta cagaaattgg
     1201 cgtacgcgat cgtttgcggc tggcgcggaa ttcaatctga agtcgcggct tgctccatag
     1261 agaccaattt tgcctggaga tttttctgtg atttcatatc ttgctttgaa gtacttcaga
     1321 attcctggtt gctcgcccgg cttgatgaat ccgtcacgtt ccattgcatc tagaaccatc
     1381 tcctgtttgt gcagagatag tttgatgttg ttcatgggac tgtatcgtac ggggctcggg
     1441 aacaaaccta cgatcatggc cgcttccgca gcatcgagcg acgttggtgg tttgcggaag
     1501 tagaaccacg atgcttcttc cgctcccagt ctgccttctc ccatgtagat gcgattcata
     1561 tacaaacata gaatttcttg tttgcttaac tttgattcta tgaggaaggc cgcgaatgtt
     1621 tcgtaaatct tgcgaatgag gcctggagtg cttctgtcta tgtacagatt tcgcgccacc
     1681 tgttgagtga tgctccctcc gccttcgcga acgctgaaac ggcggatatt gtttataaat
     1741 gctcgaagga tgccgcggta ggaaactcct ccatgcgtga agaaatcttt gtcttcggaa
     1801 gacacagcag ctcttgaaag ccagaccatc tgatttgtac atgcatcgag cgggatccgg
     1861 gattctctct gcggcaggta ttctccaatg agtgaatcat cgtttgcgag aatctgaatc
     1921 atgggtttcg caggacgatc gccctggccc cgcagccaca ttttctcttt ttccagagac
     1981 gcagagattt cagcgcgatc gtagaggaac cgcacgaaaa cgcccagagt cagaactgtg
     2041 agggcaaaaa agcaaattgc gaatgtgcga atcagaatgg ttttccacga tcttttgagt
     2101 ctctctgtcc aggggcgaag gactggctcg atcctggccc acagaggcgg aagtttttct
     2161 gatgccatgc ggatggccca ggctaccgca tcttgcatgc ggtgagcgag cgcgcgagcc
     2221 acctctaaga ttctgttcag atggttctcc ttcagttaga ctgcggcgtc gtggaaggtt
     2281 gttcgagcgg ctggattcct tcgtcacgct ttggttctac gggaggattg tccgggggaa
     2341 gatctccgct ggcaagttcc cgcgccagct ggttgatggg ggccggcgca ctcgatgcca
     2401 ggtccttcag tgtcgctgtg gcttcctctt ttgttttgct cgatgcttta ggatttttat
     2461 aaatttctgc agcctgttca aatcgcaccg ctgcggttac aagttcagta tctttggaaa
     2521 ttcccgctcc acctttcagc cagccgcgga aatagctcgc agagttagct ccgcccgaaa
     2581 cctccaccca ttctccatca ctgaattctt cgttctctac gaacgccaca gtgccaacag
     2641 taagatttga atttcctctc gcaaggccgg atgtgatgtt tggacgctga tagagtttcg
     2701 catctccagt aatgacgact ccgctttttg caagatagct tgcgaccacg tagccttcct
     2761 gaccgccggc gattttgatc ttcgcgtatt ctactgcggc gccctgagct ggcttgtacg
     2821 attcaattct gtcgagagtc accacttcgc ctttctccag agaaagaggc agcgcgcttt
     2881 ttggatctcc cattgttttg agaacgccca tgcgaaacct ggtcactctt gtttctgtgg
     2941 attgcgaaat cttgccaccc tgcttgcatt ctgcggtgaa gagtgtgagc gcggcgagga
     3001 aaaggacgaa gggttt
//
LOCUS       DAORFF010000490         3016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_21792_length_3016_cov_3.219858, whole genome shotgun sequence.
ACCESSION   DAORFF010000490 DAORFF010000000
VERSION     DAORFF010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21792_length_3016_cov_3.219858"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..929
                     /locus_tag="PKK76_16205"
     CDS             36..929
                     /locus_tag="PKK76_16205"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisS family protein"
                     /protein_id="HNL70075.1"
                     /translation="MAAPAADAEILSLLIDILTAFGAKQGDFLLRVSDRRLFDSYVQH
                     TDKEVRRSIGRLLDKREKIGEEAFQEELRRTADGPLVDRVNSFLSIRLEDLEKLGQKG
                     EIDAAVGGHLSAVSDILSAQGKSAFLSFDPGIVRGFDYYTGLIFEAQDLNPENRRAIC
                     GGGRFDRLLGQMGKNELPAVGFGLGDVTLEQFLRSRGFLADASPKKGCFFILFPESRD
                     ASLKIASDLRKAGVLVETSLEPSVKIGKQMEAADKKGRRFVLMMGGDELARGVVRVKD
                     LATGDQTDVKPSDLSSHLNRA"
     gene            945..1619
                     /locus_tag="PKK76_16210"
     CDS             945..1619
                     /locus_tag="PKK76_16210"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNL70076.1"
                     /translation="MRFRIVIFTFCFVLSCARVAAVPPSTRVVFFGDSITEIGVRQGG
                     YIQRMQTILSERGLSDRFELMGAGVSGDKIYDLYLRLEDDVFARNPQVVVVWVGVNDV
                     WHKQIHNTGTDAVKFKRFYVALIRKLQARNMRVILVTPMGVGEKRNLANPLDQELNQY
                     SALIRDLAVQYHCDLVDMRAAFLKYVGSHNPLDRESGILTYDRVHPNDTGNQFVANEM
                     LRVLLR"
     gene            complement(1796..2491)
                     /locus_tag="PKK76_16215"
     CDS             complement(1796..2491)
                     /locus_tag="PKK76_16215"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNL70077.1"
                     /translation="MGFEIDPGFQRMMIRMSLAALCGLAIGMERGYRGKAAGVRTNLL
                     ICLGAALFMIVSEEVAYEAKSNGFPGPDPARIAAQVVTGIGFLGAGAILTNRGTVTGL
                     TTAASIWCVSAIGLCAGAGLYRLAMAATLGILFTLEFFTFFERRIRTTRFRYMRLEVT
                     IKKEARVQEVRRVLRNLKIQFSNEETRNILGETHYIATLYYKGELEKMIEDELMLVSG
                     VRDVNFLGEGMDT"
     gene            2556..>3016
                     /locus_tag="PKK76_16220"
     CDS             2556..>3016
                     /locus_tag="PKK76_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNL70078.1"
                     /translation="MLTVRVIPCLDIKDGRVVKGVQFVDLKDAGDPVEQADYYAKSGA
                     DELCFLDITASHENRDIIVDLVRRVAERIFIPFTVGGGLRTLEDMDRILHSGADKVSL
                     NTAAFLNPEVLSQGARKFGSQCIVCAIDARKSGQDWEVYLSGGRKATGRSAL"
BASE COUNT          712 a          716 c          851 g          737 t
ORIGIN      
        1 gggtgagccg gttcgggttg acgtgcagtt gataggtggc cgcgcccgcg gcggacgctg
       61 aaatcctgag tctgttaatt gacatattga ctgcgtttgg tgcgaagcag ggagactttc
      121 ttcttcgcgt aagcgatcgt agattgtttg attcttatgt gcaacacacg gacaaagaag
      181 tcaggcgatc gattggcagg ttgctggata agagagaaaa aattggagaa gaagcgttcc
      241 aggaagaatt gcgtcgcacg gcggacggtc cgctcgtgga tcgcgtaaat tcttttctgt
      301 ccatccggct tgaagatctg gagaagctgg gccagaaggg agaaattgat gcagccgtgg
      361 gcggccatct ctctgcagtc agtgatatac tatcggcgca gggtaagtct gctttccttt
      421 cctttgatcc tggaattgtt cgcgggtttg attattacac tggtttgatt tttgaagcac
      481 aggatttgaa tccagaaaat cgaagggcca tctgcggcgg cggcaggttc gacaggttgc
      541 tcggacagat gggaaaaaac gaactgccgg ctgttggttt tggtctgggg gacgtaacac
      601 tcgaacagtt ccttcgcagc cgtggctttc tggcagatgc gtcacccaag aaaggttgtt
      661 tttttatact attccctgaa agcagagacg cttcccttaa aatcgcatct gacttgagaa
      721 aggcgggcgt gctggtagaa acatctcttg aaccatctgt aaagattgga aagcaaatgg
      781 aggcggcgga taagaaaggc cgcagatttg tgctcatgat gggtggagat gaactggcca
      841 gaggagttgt gagagtaaaa gatcttgcaa caggggacca gacggatgtc aaaccatcgg
      901 atctgtcctc tcacctgaat cgcgcttagt attgagcgat cgctatgcga tttcgaatcg
      961 taatcttcac tttctgcttc gtcctttcct gcgcgcgtgt ggctgcggtc ccgccatcta
     1021 ccagagttgt ttttttcggc gattcgatca ctgaaatcgg cgtgaggcaa ggtggataca
     1081 tccagcgcat gcagaccatt ctatctgaga gaggactttc ggatcgcttt gagcttatgg
     1141 gagcaggagt gagcggagat aaaatctatg atctctactt gcgtctggaa gatgatgtat
     1201 ttgcgcgcaa tccccaggta gttgtggttt gggtgggagt caatgacgtc tggcacaagc
     1261 aaatacacaa tacaggcaca gacgcagtca agtttaagcg attctatgtc gcgctcattc
     1321 gtaaattgca ggcgcgaaat atgagagtga ttctggtgac accgatgggt gtgggggaaa
     1381 aaagaaatct ggcaaatccg ctcgaccagg aactcaatca gtactctgcc ctcattcgcg
     1441 accttgcagt tcaatatcac tgcgatcttg tggatatgag agctgctttt ttgaagtatg
     1501 tgggctctca caatcctctg gaccgcgaat ccggaattct aacgtacgac agagttcatc
     1561 caaatgacac gggcaaccag ttcgtcgcaa acgaaatgct gcgagtactt ttacgttgat
     1621 atgcggcacg aaaggctcag aaccttgccg acacttagcc tctcagcgta cgaaccggca
     1681 tgtcttgccc aattgcggag gcaggatgcc agagccccgg cgcgagtcgg acatggacga
     1741 ccgcgtctcg cgccggttgg gcaagggcat gtcgtttcgc aaaggccaga gaggactaag
     1801 tgtccattcc ttctccgaga aaattcacat cgcgcacacc ggacacaagc atcagttcat
     1861 cctcgatcat tttttccagt tcgcctttgt aatacaatgt tgctatatag tgagtttcgc
     1921 cgagtatgtt gcgcgtttct tcgtttgaga attggatttt tagatttctt aaaacgcgac
     1981 gcacttcctg cacacgggct tctttcttaa tggttacttc gaggcgcatg tagcgaaagc
     2041 gtgtggtgcg aattctgcgc tcaaagaacg tgaaaaactc cagcgtaaac aggatgccaa
     2101 gagtggcagc catggcgagt ctgtagagcc cggcgccggc gcacagtccg atcgcagaaa
     2161 cacaccagat actggcggcc gttgtgagac ctgtgacagt tccgcggttg gtcaggatcg
     2221 ctcctgcacc cagaaagcca atgccagtga ccacctgggc tgcgatgcgt gcagggtctg
     2281 gtcctggaaa tccattggac ttcgcttcgt aggccacctc ttctgaaacg atcatgaaga
     2341 gtgccgcgcc caggcagatg agcagattcg ttcgcactcc cgccgctttt ccgcggtaac
     2401 cgcgctccat tccaatcgcc agcccgcaca gcgcggcaag cgacatgcga atcatcattc
     2461 tctgaaaccc aggatctatt tcgaagccca tgattagaat ttcggcgagt tcctggtttt
     2521 gcttgcccca aatcgaggcg ggtaaagata gtcgtgtgct caccgttcgc gtcataccgt
     2581 gcctggacat caaagacggc agagtcgtca aaggtgttca gtttgtagat cttaaggacg
     2641 caggagatcc ggtggagcag gcagactact atgcgaagtc gggagcagac gagctctgtt
     2701 ttctggacat cacagccagt cacgaaaacc gcgatatcat agtcgatctc gtgcgtcgcg
     2761 tagcggagag gatcttcatt ccgttcaccg ttggcggggg attgcgcact cttgaggata
     2821 tggaccgcat ccttcattca ggagcggaca aagtttctct gaacactgcc gcttttttga
     2881 atccagaagt cctttcgcag ggcgcgcgaa agtttggtag tcagtgcatt gtgtgcgcca
     2941 ttgacgcgcg aaagtcgggc caggactggg aagtgtattt gagcggtggt agaaaagcaa
     3001 caggcaggtc tgccct
//
LOCUS       DAORFF010000491         3015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_21806_length_3015_cov_3.487162, whole genome shotgun sequence.
ACCESSION   DAORFF010000491 DAORFF010000000
VERSION     DAORFF010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21806_length_3015_cov_3.487162"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1503)
                     /locus_tag="PKK76_16225"
     CDS             complement(184..1503)
                     /locus_tag="PKK76_16225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNL70079.1"
                     /translation="MKLPQKLALSNVRRTPFAQIAKALGNYPSHHLGLAVAKDIIQNS
                     GVKKETFDGVFVGEGFPFAPNAARVIASLLEMPVEKPALTLSNNCVSSFEAVGEAARR
                     ITLGEGNVYLALGEESQTSIPFILKGARMNKKAGSVDKLLKAFPTGLPEGVELMDSLD
                     AGLGDGETSYGMPVTAEIVAQNYGLSRELQDKFAYQSYKRAFEATQAGKYDKYLVPMK
                     DDEGGMLTHDEAVLLREGIVKNPSRMDKAMLLFENPQFKFEQFKQNYGKFLTQSHGPT
                     VTIFNACARSDGAAGAIVSSVDRAKELGLKPSVYLTGFCMRGVHPNFMGIGQAEASLK
                     LIEELGLKIGDIDQIEIHEAFAATAVGALEEMKKRSGLGWEKMFEEGRINAFGGSIAI
                     GHPFGATGIRLVANAAMALEHNPAINRVLVTACAHGGIAGAMILERA"
     gene            complement(1750..2271)
                     /locus_tag="PKK76_16230"
     CDS             complement(1750..2271)
                     /locus_tag="PKK76_16230"
                     /inference="COORDINATES: protein motif:HMM:NF022517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HNL70080.1"
                     /translation="MTSGKIMSVPLPPSSVLYAPSIPYDYADSYSAVLSDSSMTATDA
                     AWEFFGQAPRWVGTLMRMRNSLVSLFGLKTGQSQKPVERRALAPGQKVGLFRIYSASP
                     TEVILGEDDRHLNFRVSVLVDPGDQSKKKLVVSTVVHYQNGWGQIYFFFVRPFHRIIV
                     PAMFRRMITRLAA"
     gene            complement(2268..2675)
                     /locus_tag="PKK76_16235"
     CDS             complement(2268..2675)
                     /locus_tag="PKK76_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70081.1"
                     /translation="MRFSVFFLTALFLVFLPAAILSFFMHHPDTWTEESVVGFALAVV
                     AFGLIPNGIPALLLVFLMIWKKVASVRDFVQRRAVLCSFLAALASVLVIHWWTFADPA
                     EAGLLIHSLAVVFFGGIAVLFGFICGSWIEKKS"
BASE COUNT          801 a          817 c          739 g          658 t
ORIGIN      
        1 caatatcagc ggtcgtgctg tagagctcga tggcagatac tgctgaaaca gaaaggaagg
       61 tcagtaaaaa cgcacgcagc atatcctctt attagacgtc aaacgaggaa gaaaagtaaa
      121 aacgcacaaa aaaaccggga agaaaaactc cccggtttcc tttttcgtac gcaaaaaaat
      181 ggatcaggcg cgttccagaa tcatggcacc cgcaatgcca ccgtgggcac atgcagttac
      241 cagaacacga ttgatcgcag gattgtgctc gagcgccata gccgcgtttg caaccagacg
      301 aatccctgta gctccgaatg gatgaccaat tgcaatggat ccaccaaaag cgttgattcg
      361 gccttcttcg aacatctttt cccatccgag accggaacgt ttcttcatct cttcaagagc
      421 acccactgct gtagcggcaa atgcttcatg gatttcgatt tgatcaatgt cgcctatctt
      481 tagacccagc tcttctatga gcttgaggct tgcttctgcc tgaccaattc ccatgaaatt
      541 tggatgaacg cctctcatgc agaaacctgt gaggtataca gaaggcttca gtcccagttc
      601 cttcgcgcgg tctacactcg atacgatggc accggccgca ccgtctgaac gagcacatgc
      661 gttgaagatc gttacagttg ggccatggct ctgagttaga aacttgccgt agttttgttt
      721 gaactgttcg aatttgaact gagggttttc aaacagcagc attgctttgt ccatacgaga
      781 aggattcttc acaatccctt cgcgcagaag cactgcttca tcgtgggtga gcattccacc
      841 ttcgtcgtct ttcattggaa ccaggtattt gtcgtacttt cctgcctgag tcgcttcgaa
      901 cgcgcgcttg taactctggt aagcaaactt atcctgcagc tcacgcgaca ggccgtagtt
      961 ttgcgcaacg atctccgcgg tcacaggcat accgtaactt gtttccccgt ctccgagacc
     1021 tgcgtccaga gagtccatga gctccacacc ttctggcaga ccggtaggaa atgctttcaa
     1081 aagtttatca acgcttcccg ccttcttgtt catgcgggcc cccttcagaa tgaaaggaat
     1141 ggaagtttgc gattcctctc cgagagcaag atacacgttg ccttctccca gagtgatgcg
     1201 acgcgccgct tcaccgactg cttcaaagga agacacgcag ttgttggaaa gagtgagagc
     1261 aggtttctcc acaggcattt ccagaagact cgcaatcacg cgagccgcgt taggcgcaaa
     1321 tggaaatcct tctccgacaa aaacgccatc gaatgtttct tttttcacac cggaattctg
     1381 aatgatatct tttgcaacag ccagacccag atgatgggac ggataattgc cgagcgcttt
     1441 agcgatctgc gcgaaaggag tacgccgcac attggacagg gccagttttt gaggtagttt
     1501 cataggaaca catggccgcc aaagttttta cggtcaaggc ttaaaaccat ccacgcgctc
     1561 gagaggacct cccgcattat gtctcgtctc atttcgtgca aagaagagtt cggggcgcct
     1621 cctgccctgc ggtcaggacc gcgctcgcac gggccggcca gaatttttgg tgcgatcagc
     1681 ctcacaacaa ttcggaagtg aaacgaacgc aggccgcccg cttgatcgct ggcgctttcc
     1741 actgcaacgt tatgcggcca atcgagtgat catgcgacga aacatggcgg gaacaatgat
     1801 gcgatggaac ggacgcacga agaaaaaata gatttgtccc cagccgttct ggtaatgcac
     1861 tacagtagac acaacgagct tctttttgct ctgatctcct gggtctacca ggacagagac
     1921 acgaaaattc aaatgacgat catcttcccc gaggatcact tccgtgggac tggccgaata
     1981 gatgcgaaag agccccactt tttgtccggg cgcgagagct cgtctctcta ccggcttctg
     2041 actctgacca gttttgagcc cgaaaaggga aacaagggaa ttgcgcatgc gcatgagagt
     2101 tcccacccat cgcggagcct gaccaaaaaa ctcccacgcc gcatccgtgg cagtcataga
     2161 cgaatcagaa agaacagccg aatagctgtc tgcatagtcg tatgggatac tcggagcata
     2221 gagaacagaa gaagggggca gaggtacgga cataattttc ccgctcgtca tgatttcttc
     2281 tctatccagg aaccacagat gaatccaaat agaacagcga tccctccgaa gaacaccact
     2341 gccaatgagt gaatcaacag ccccgcctcc gccggatctg caaaggtcca ccagtggatc
     2401 accaggacag aagctagagc agccaggaaa gaacaaagca ctgcccttct ctgcacaaag
     2461 tcgcgaacgc tcgcaacttt cttccagatc atcaagaaga caagaagcag agcaggaatt
     2521 ccgttgggaa tgagcccgaa cgcaaccact gccagcgcaa agccaacgac gctctcctca
     2581 gtccatgtat ccgggtgatg catgaagaaa gaaagaatgg ccgcaggcaa aaaaaccaga
     2641 aaaagggcgg tcagaaaaaa tacagagaag cgcaagcctc agccttcccc ctgagaagat
     2701 gcaggcaacc aaaaaattcg cccatggaaa ggggtaacgt tatgcggcat accggagacg
     2761 gaaacccccg accctcggat tcgcaagcat ccgggcccgg agtttcgcgt gacagacaat
     2821 ctgtctgcgc ctacgtgggc tcatgcgact gtgtgtattt ctactctgcc tgtttcttac
     2881 aacctgtgct gcggcggagc ccgcggactc tcacgatcca ctcgcagctg tcatcgaagt
     2941 atccaggaag tatcaggttc ttccaaacaa tacgtatcga gtggttcagg gccgcgaatt
     3001 gaagctggat gtatt
//
LOCUS       DAORFF010000492         3006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_21917_length_3006_cov_3.399526, whole genome shotgun sequence.
ACCESSION   DAORFF010000492 DAORFF010000000
VERSION     DAORFF010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21917_length_3006_cov_3.399526"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..131)
                     /locus_tag="PKK76_16240"
     tRNA            complement(47..131)
                     /locus_tag="PKK76_16240"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(95..97),aa:Ser,seq:gga)
     gene            complement(256..1527)
                     /locus_tag="PKK76_16245"
     CDS             complement(256..1527)
                     /locus_tag="PKK76_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002103954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNL70082.1"
                     /translation="MSRRLGEILRKILKGPPLKFNPFVMSRVFYLWLFLGVAAGASAG
                     IYWIVLEQIIYFASAVEDWKVIPLMGGAGLLAGLIIHWIGDPGEIQLIVNNIRFRGGR
                     LDTKHNVSMILSSLLCVGTGGSLGPEAPLVQVTGSVGTWISERLRLKGEEFRSMTIAG
                     MAAGFTALFGAPLGGSLFALEILHHRHVVEYYEAIIPAIVSSLAAYTVFALLIHLGFG
                     PVWNVPAYTTQSVLDFGLAVVFGTVAAAVGWVFISVTNHLRVFVREISAPIFVKTCAG
                     GFLLGAISFYMPLSRFFGHREIELLFQGNFTIGFLIALLMAKLGAIAITVTTGWRGGF
                     IIPLFFMGAVLGLLLKQIFPSISLPLAAVCCMASLNSCVTRTPLSTTLLLSTMTGFSH
                     FVPILFASMTGYFLAPKTPFIITQQRPAGET"
     gene            complement(1645..2814)
                     /locus_tag="PKK76_16250"
     CDS             complement(1645..2814)
                     /locus_tag="PKK76_16250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL70083.1"
                     /translation="MRPAWPELRALALNHFAGTFGFALSFPFLSVYCHKILGVSITET
                     GIAFGILGLVRALSQYLGGELADRGSVVRLMSISLFTRSFLFALVALGIYFRIGFYPI
                     ILLSFFQTVAGSVHQMASQNAVSILLPDEQKMHGFAVTRAASNIGFAAGPAAGGWLVG
                     YSYVIPFTLTAVLLLVSIVPLMSLNVRGRTAIARTSGEKETLPMSFIVYLFAVFLVAS
                     VMAQIITPMSLYLVGMKGIGESNLGWLYALNGLIVGLLQVKMMHVFRAISYLRVMIIG
                     SITYFVGYAALGMQDGLPWYVFTTMLITLGEMLVAPPSVAHAERIAPTHRTGRYLGLV
                     GFSAALGWAFGPTYGSWLLEQTDNSTLAWCIIALPALMSAAIMMILDKKTNIART"
     gene            complement(2795..>3006)
                     /locus_tag="PKK76_16255"
     CDS             complement(2795..>3006)
                     /locus_tag="PKK76_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNL70084.1"
                     /translation="EAREKGAALVVYVIPPKLTLESLAQARELGMKKVWVQPGAGDEA
                     VREYLDANSFEYLMDACVMVETRVA"
BASE COUNT          835 a          793 c          775 g          603 t
ORIGIN      
        1 tgcccgtgta cacgcacaga ttgatggatg cggctgtaac taacttcgga gagggcgggg
       61 ttcgaacccg cggtacattt ctgtacacat gctttccaag catgcaccat cgaccactcg
      121 gacacctctc ctggtccgga ccagaccgcc agagaggggc tcatggtcaa ggacttcacg
      181 gggcaatgct tgactgacga gcgcacctgg aagatcgcac gtgacaaggc gcatgtcaga
      241 ttctaatctc gagtcctacg tctcaccggc cggcctttgc tgagtgatga taaatggtgt
      301 ttttggtgcc aggaagtagc ctgtcatact ggcgaacaga ataggaacaa aatgagaaaa
      361 gccagtcatc gtggagagca gaagggtcgt gctcagcgga gtgcgcgtaa cgcaagagtt
      421 gagcgaagcc atgcagcaaa cggccgccag gggcaggctg atgctcggaa aaatctgttt
      481 cagaagtaag ccaaggactg cgcccataaa gaaaagaggg atgatgaatc cgccgcgcca
      541 tcctgtcgtc acagtgatgg caatggcgcc taactttgcc attaggaggg cgattagaaa
      601 cccgattgtg aaatttccct gaaaaagaag ttcgatctca cggtgaccaa agaagcgcga
      661 aagaggcatg tagaaagaga tagcacccag aaggaaaccg ccggcacatg tcttcacgaa
      721 aattggcgct gagatctctc taacgaacac gcgaagatga ttcgtcactg agatgaagac
      781 ccagccaacc gccgcggcaa cggttccaaa cacaacggca agtccaaagt caagaacaga
      841 ctgtgtggta tacgcgggaa cattccagac aggaccaaac cccagatgga tcaagagcgc
      901 aaacacggtg taggctgcaa gactggaaac aatggcaggt atgatcgctt cgtaatattc
      961 caccacatgc cgatgatgca gaatttccag agcgaataga cttccgccaa gtggtgcgcc
     1021 aaatagtgcg gtgaatcctg ctgccattcc ggcgatggtc atcgagcgaa actcttctcc
     1081 tttcaatcta agtctttcgg aaatccaggt gcccactgaa ccagtcacct gcacgagagg
     1141 tgcttctggt ccaaggctcc ctcctgtgcc cacacacagg agagaagata gaatcatcga
     1201 aacattgtgc ttcgtgtcca gtctcccacc gcgaaagcga atgttgttta cgatgagttg
     1261 aatttccccc gggtcgccga tccagtggat gatcagaccg gccagaagcc ccgcacctcc
     1321 catcaaggga atcactttcc agtcttcgac tgccgatgcg aaatagatga tctgttctag
     1381 tacgatccaa tagattccag cactcgctcc cgctgccact ccaagaaaca accagagata
     1441 gaagactcta ctcataacaa acggattgaa tttaagagga ggccctttca gaatcttacg
     1501 aaggatttct cccaatcggc gtgacattca gtcagcgcga aacaagcgtt ctgcggagcg
     1561 agccatattc ccggatgtgg acttgatgag cgtcaagagc ctggcaggag tgcggcagag
     1621 agagattcag taaatcgcct gacgttatgt tcttgcaata tttgtttttt tgtccaggat
     1681 catcatgatc gcagcagaca tcaaagccgg gagcgcaatg atgcaccagg caagagtcga
     1741 gttgtcagtt tgttccagca accagcttcc gtacgttggc ccaaaagccc atccgagcgc
     1801 ggccgaaaag ccaacgagtc caagatacct tcctgttctg tgggtcggtg cgattcgttc
     1861 cgcgtgcgcg acggaaggag gggcaacgag catttcgccc agagtaatca acatagtagt
     1921 aaagacgtac cacggaagcc cgtcctgcat gcccagagcc gcgtatccaa caaaataggt
     1981 aatgctgccg atgatcatca ctcttagata tgatatagcg cggaatacat gcatcatttt
     2041 gacctggagt agccccacta tcaagccatt gagcgcgtag agccagccta gatttgattc
     2101 acctatgccc ttcattccta caagatacag agacatcggg gtgatgatct gcgccatcac
     2161 actggcaaca agaaagacag cgaatagata gacaatgaag gacattggta gcgtttcctt
     2221 ttcgccagaa gtgcgagcga tggccgtgcg cccgcgcacg ttaagcgaca tcaaaggcac
     2281 gatcgaaacg agcaatagaa cagccgtcaa tgtaaatggt atgacataag aatagccgac
     2341 gagccaccct cctgccgccg gtcccgccgc aaaaccaatg ttagacgccg ctctcgtgac
     2401 tgcaaaccca tgcatctttt gttcatctgg cagcaagata gacaccgcgt tttgagatgc
     2461 catctggtga acggaacctg ccactgtttg gaagaacgag agtagaatga tgggataaaa
     2521 gccaattctg aaataaatcc cgagcgcaac cagtgcaaag aggaaagacc ttgtgaagag
     2581 cgaaatggac atgagacgca caacagaccc gcggtccgcc agttcccctc cgaggtattg
     2641 cgaaagagcg cgcaccagtc ccagaattcc gaatgcgatt cctgtctccg tgatggacac
     2701 tccaagaatc ttgtggcaat acacggaaag aaatggaaac gatagtgcga agccgaaagt
     2761 accggcgaag tgattgagtg cgagtgcgcg caactcaggc cacgcgggtc tcaaccatca
     2821 cgcacgcatc catcaagtat tcgaaagaat tcgcatccag atactcacgc acagcttcat
     2881 ctccggcacc aggctggacc cagactttct tcatgccgag ttcacgcgcc tgggccaggc
     2941 tctccagtgt gagtttggga ggaattacat acacaacaag agccgctcct ttttccctgg
     3001 cttctc
//
LOCUS       DAORFF010000493         3003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_21961_length_3003_cov_4.587856, whole genome shotgun sequence.
ACCESSION   DAORFF010000493 DAORFF010000000
VERSION     DAORFF010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21961_length_3003_cov_4.587856"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PKK76_16260"
     CDS             complement(<1..197)
                     /locus_tag="PKK76_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNL70085.1"
                     /translation="MLMDYKTAGVDTHAGQQFVKLIGPAVASTKKPHVLGGLGGFAAM
                     SDLSFLKEYKRPVLLSTTDGVG"
     gene            complement(263..1435)
                     /locus_tag="PKK76_16265"
     CDS             complement(263..1435)
                     /locus_tag="PKK76_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HNL70086.1"
                     /translation="MRKFIAILLAVAVSGLSAEALQYKLSWAKDANAKSYEVEVSPAQ
                     DFTKVVQNLKTAVNEAKFSLEPTEPKYFWRHRSVDAAGKAGAWSAAAELEKPAAVAST
                     DTIPPLQISTDALKQTGAPMPDVYTLPGGKALVVRFNPKDSISGMGEVYAKVNNGSVS
                     LVPNAEMRLTADGAYVIEWYAIDRAGNKSPIQIRRIYIDSTAPTLTRTIEGSKIATDG
                     SVAKTAKAVLTARDEGTGVDKIEWRSGTEGEFKTYETAIALASIATNDTGVIQYRAKD
                     KIGNETAIQTYSFRIDSVAPQLPTIFQGLQEPYRIPREGLSVADFPKNAIVEYKIDDS
                     DFRKLEPGDRIVIDKEGEHTVTIRVTDEVGNVTEKTYKVLVDNTPPKSELRAEIAP"
     gene            complement(1581..2882)
                     /locus_tag="PKK76_16270"
     CDS             complement(1581..2882)
                     /locus_tag="PKK76_16270"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HNL70087.1"
                     /translation="MKKFKFASDFLWGTATAPTQIDGGDNASDWYEFCQQKGRILDGS
                     SSFTGCDHWNRYKEDFDLMKKMGHNAYRLGADWSRFEPQPGSFDHKALDHFRQMLQYF
                     KKKKIKPLLTLMHFAVPSWWLKKGGWTKEENIEDYMRFARWLIENTGDLVHEYITINE
                     PVVYSVIAYMDGRWPPGEGGLLGYFRSLTVQRNLALTHFQLYDLIHSAHARRNWKKPA
                     VSIAKHMRVTDPENPASGLDVNAARTVDEFFNLAFCDTIQSGQMSGGLGKGERAHDGK
                     AWDFFGMNYYSRDIVCFDLLRPHQAFTKIQVQPGSPRNDLNWEIYPEGMKRTLMKVHD
                     RYGLPIRITENGIADAKDAQRPQFLIDHISAMESAAQEGVKIDGYYHWSFMDNFEWAE
                     GYTARFGLVEVDFKTMQRKPRASARIFSKIIKNRGTAGIRA"
BASE COUNT          622 a          743 c          761 g          877 t
ORIGIN      
        1 cctacaccgt ccgttgtgga aaggagtacg ggtcttttgt attcttttag aaaactcaga
       61 tcgctcatgg ccgcgaatcc gccgagtcct cccagaacgt gaggtttctt cgtactcgca
      121 actgcaggac caatgagttt tacaaattgt tgtcctgcat gagtgtcaac gccggctgtt
      181 ttataatcca taagcatcca ttttctttag caaaaaaaaa ggccggtttc ccggcctttt
      241 ctcttttaga gatcagagtt gatcaaggag caatctctgc tctgagttcc gatttaggcg
      301 gagtattgtc tacgagcact ttgtaagtct tctctgtcac gtttccaact tcgtcggtta
      361 cgcgaatggt tacagtgtgt tcgccttctt tgtcgattac gatgcgatct cctggttcca
      421 gttttctgaa atcagaatca tcaatcttgt attcaacaat agcattcttt gggaaatccg
      481 caacgctcag accttcgcgt gggatgcggt aaggctcctg cagtccctgg aagatggttg
      541 ggagttgtgg tgcaacgctg tcgatgcgga atgagtaagt ctggatagcg gtttcattcc
      601 cgatcttgtc cttcgcgcga tactgaatca ctccagtgtc gtttgttgcg atggatgcga
      661 gggcgatggc agtttcgtag gttttgaatt caccttctgt tccgcttctc cactcgattt
      721 tgtctacgcc tgttccctca tcgcgagcag ttagaactgc tttcgcagtc tttgcaacgc
      781 ttccgtcggt agcgatcttg cttccttcga tggtgcgagt cagtgtaggt gcagtgctgt
      841 cgatgtagat tctacggatt tgaattgggc ttttgtttcc tgcgcgatca atcgcatacc
      901 attcaatgac gtatgcgcca tcagcagtca gtctcatctc tgcgtttggc acgagagaaa
      961 cggaaccgtt attcactttc gcgtatactt ctcccattcc ggagatggag tctttgggat
     1021 tgaaacgaac taccagtgct tttccgcctg ggagtgtata cacatccggc ataggcgcgc
     1081 ccgtttgctt gagggcgtct gtggagattt gcagaggtgg gattgtatct gtgcttgcga
     1141 cagccgctgg cttttcaagt tctgcggccg ctgaccaggc accggctttt ccagcagcgt
     1201 ctacgctgcg gtgacgccag aaatactttg gctctgttgg ttccagactg aacttagctt
     1261 cattgacagc tgtcttcaga ttctgaacta ccttggtgaa gtcctgtgcc ggggagactt
     1321 ctacttcata actttttgca ttggcgtctt tggcccagct gagcttgtac tggagtgctt
     1381 cagcggacag cccgctcacg gcaacggcga gcaaaatggc gataaatttg cgcataggtt
     1441 cctcggaata cctttttggg gaagttttgt cagccagaga atgacgtcaa ctcaggattg
     1501 catcggcgaa tggtttttgc ccaaacggtt tctgaatccc gaattatgtc gcattagtag
     1561 agccacgatc ctggatcgga tcaggccctg atccccgccg tgcccctgtt tttgatgatc
     1621 ttgctgaaga ttctggcaga tgctctgggt ttgcgttgca tggttttgaa atccacttcc
     1681 acgaggccaa atctggcagt gtaaccttcc gcccattcaa agttatccat gaaactccag
     1741 tggtagtatc cgtcgatttt gactccttcc tgagccgcgg actccatggc ggagatgtga
     1801 tcgatcagga actgtggcct ttgtgcgtcc tttgcatctg caattccgtt ctcagtgatt
     1861 cggatgggta gaccgtagcg atcatgcacc ttcatcaagg ttctcttcat tccttctgga
     1921 tagatttccc agttcaaatc gttgcgagga gagcctggct gcacctggat cttagtgaaa
     1981 gcctggtgcg gtcgcagtag atcaaagcac acgatgtccc tggaataata gttcatcccg
     2041 aagaaatccc atgcctttcc gtcatgggct cgttcccctt tcccgagtcc tccactcatc
     2101 tgcccggatt gaattgtatc gcagaatgca aggttgaaga attcgtccac agtgcgcgcc
     2161 gcatttacgt ccagaccgct tgctggattt tctggatccg tcactctcat atgctttgca
     2221 atggaaacag caggcttctt ccagtttctg cgtgcgtgcg cagagtgaat gagatcgtag
     2281 agctggaagt gcgtaagggc gaggttccgc tggactgtca aagaccggaa gtaaccaagc
     2341 agtccgccct caccgggtgg ccatcttcca tccatgtagg cgatcacgga atagacgact
     2401 ggttcattga ttgtgatgta ttcgtgcacg aggtcgccag tgttttcgat caaccagcgc
     2461 gcgaagcgca tgtaatcttc tatattctct tcctttgtcc agccgccttt cttcagccac
     2521 cacgagggca ctgcgaaatg catgagagtc agtagaggtt tgatcttctt ctttttaaag
     2581 tattgcagca tctgacggaa gtgatcgaga gctttgtgat caaaggagcc tggttgcggt
     2641 tcaaaacgag accagtctgc gcccagtctg tacgcgttgt gacccatctt cttcatcaga
     2701 tcaaagtctt ctttgtatcg attccagtga tcgcatcccg taaacgaaga ggatccgtct
     2761 agaattctac ccttctgctg gcagaactcg taccagtctg aagcgttgtc tccgccgtcg
     2821 atttgagtgg gtgctgtggc cgtgccccag aggaagtcag atgcgaattt gaattttttc
     2881 attgtgaacc gcgaagcttt tcaaaaggta gggttgccca atgagtcaac catttatacg
     2941 tatcgcgctt gtttctgtgc tttttttcgt gcatcccggt ctttcggaga atcgcaaggc
     3001 gga
//
LOCUS       DAORFF010000494         3000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_22001_length_3000_cov_3.324958, whole genome shotgun sequence.
ACCESSION   DAORFF010000494 DAORFF010000000
VERSION     DAORFF010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22001_length_3000_cov_3.324958"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKK76_16275"
     CDS             <1..638
                     /locus_tag="PKK76_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNL70088.1"
                     /translation="IGVPPARPETGYGYIEAGERHADGAHEISSFKEKPSAHQAGEFL
                     SQGGFFWNAGIFAWKGSVIVDEFQKHAPEILEPIARAFGNDGEMKKAFHHLHGTPIDI
                     AILEKSKRVVMVPATFQWDDVGSWLSLPRILKADDAGNINVSERGVITANDAERNIVL
                     ADKKLIALLGVKDLIVVETDDVLFVADEKSIGRIKELLQHMNQNSSLQKHL"
     gene            856..1575
                     /locus_tag="PKK76_16280"
     CDS             856..1575
                     /locus_tag="PKK76_16280"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNL70089.1"
                     /translation="MVGVSLLLIFMDVQAGKQIKDRVEALPDQISNRVAPEVRSQVAS
                     IPSRAAALEQPVKQSASAIFEGVQNRVQSHAPAVQKIDASSLDSVPESVMPSASPDSR
                     LKVTGGGWDLYFVAYSDRESVIKKVHRTWPQSQVGVRALLQYLQKGPSPRERGLLNPF
                     DSSVRLMSHNLSEGVLTLEFDPSLLRMGAHVIRDRMDQVLFTMTQFPEVQGVRFVVPG
                     EDRPYLNKVLKKPSRKVMSYP"
     gene            complement(1559..2395)
                     /locus_tag="PKK76_16285"
     CDS             complement(1559..2395)
                     /locus_tag="PKK76_16285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL70090.1"
                     /translation="MIYRYTIKRFRPEFDIVRCSRCGLERQSVLPQIDQMYDEDYYSG
                     KSEYSYKDERKNEKYEAYVWRARLKRIAEFVPAPADFLDVGCAYGGLALEARASGYSA
                     KGLDISKHAVAEARKRGLDAKQGRITDHLFPRGSFDVVTMIEVIEHLEDPVSAIESLS
                     RIIRPNGLLVVQTANFAGQQAVKEKDSYHYYLPGHLFYFSRNNLTELLRRHGFSRVVF
                     FPGVEFGLLPKILKSRGSFQKPSDYLKWIRMSSYHAMSKIALGDFAMTSAMVLYAIKD
                     KT"
     gene            complement(2430..>3000)
                     /locus_tag="PKK76_16290"
     CDS             complement(2430..>3000)
                     /locus_tag="PKK76_16290"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL70091.1"
                     /translation="SVQDYPDENLVRLLKKHPGQSGLDLGCGSGRHISLLRDCGYSAI
                     HATDVSETALELCRKRFRYALFHPLDARMLKKNHFEIPLPEESLDTVIAWGVLHYNPD
                     EMIASMLKEVMRILKQGGRFLGTLRSHRDTHFKNNPDMDGANLKTYTRPEAMDLLKDY
                     FADVSLGYVERIPVGSKYRVCHWIFDASN"
BASE COUNT          742 a          751 c          783 g          724 t
ORIGIN      
        1 cgattggagt tccacctgcg cgcccggaaa ctggttacgg ctacattgag gcaggagagc
       61 gtcacgcaga cggtgcgcac gagatttcat ctttcaagga aaagccttct gcccatcagg
      121 cgggagagtt tctgtctcag ggtggattct tctggaatgc ggggatcttc gcgtggaagg
      181 gcagcgttat cgtggacgag tttcagaagc atgcgcctga aatccttgag cctatcgcgc
      241 gagcgtttgg aaacgacgga gaaatgaaga aagcatttca tcatttgcac ggcactccga
      301 tcgacattgc catcctcgaa aaatcaaaga gagttgttat ggtcccggcc accttccagt
      361 gggacgatgt gggatcctgg ctttctttgc cgcggatcct gaaggcagac gatgccggaa
      421 acatcaatgt ttcggaacgt ggagtgatca ctgccaatga tgcggagcga aacatcgttc
      481 tggcggataa gaagctcatc gcacttctcg gagtcaaaga cctcatcgtg gtagaaacag
      541 acgatgttct ctttgtagcg gacgaaaagt cgattggacg cataaaagaa ctgctccagc
      601 atatgaatca gaattctagt ttacagaaac acctgtagcg gcaaaagggg atagaatgcc
      661 ttccggaaga aaaaggaagc ggatcaaaat ccgcacccac aagcgtaaga aaaaacgcaa
      721 agcaagccgt cataagaaga aaatccgctg agtgaacgga ttctgagaat cgtccgacaa
      781 taataacgga tgcttctcag aaatcccgga accccgccca ttgacacggc caactccaac
      841 cgaatcctgc tgagcatggt cggcgtaagc ctgctcctga tcttcatgga tgttcaggct
      901 ggaaaacaaa tcaaagacag agtagaagcg ctcccggacc agatatccaa tcgagtcgct
      961 cctgaagtca ggtctcaggt tgcgtcgatc ccgtcgcgcg ccgcggccct ggagcagcct
     1021 gtaaagcaat ctgcctccgc gatttttgaa ggggttcaga accgcgttca atctcacgcc
     1081 cctgcggtgc aaaaaatcga tgcgagcagt ctggattctg tgcccgaaag tgttatgcct
     1141 tctgcttcgc ctgattcgcg tctcaaagtg acgggaggcg gctgggatct ctacttcgtt
     1201 gcctacagcg accgcgaatc cgtgatcaag aaagtccatc gcacatggcc acaaagccag
     1261 gtgggcgtgc gagctctgct acagtatcta caaaagggtc cttctccaag agagcgaggt
     1321 ttgctcaatc catttgactc atccgtgcga ttgatgtcgc acaatctaag cgaaggtgta
     1381 ctgactctag agtttgatcc aagcctgctt cgcatgggcg ctcatgtgat tcgagaccgc
     1441 atggatcagg tgctgttcac tatgacgcag ttcccggaag tgcagggcgt gcgatttgtg
     1501 gttcctggcg aagacagacc ttatctgaat aaggtattga agaagccttc gcgcaaagtc
     1561 atgtcttatc cttgatcgcg tagagcacca tcgcggaagt cattgcaaaa tctccgagcg
     1621 cgattttgct catcgcgtga tagctgctca ttctgatcca tttcaaatag tcagaaggct
     1681 tttgaaacga tccgcgggat ttgaggatct tgggcaacaa accgaattcg actccgggaa
     1741 aaaaaacaac gcgagagaat ccatggcggc gaagtagttc cgttaggttg tttcgcgaaa
     1801 agtaaaaaag gtgacctggc aaatagtagt gatatgagtc cttctctttg actgcctgtt
     1861 gtccggcaaa gttagcggtc tggaccacga gcaatccgtt tggacggatg atgcgcgaaa
     1921 gactctcgat cgcagagact ggatcctcca ggtgttcgat cacttcaatc atggtgacca
     1981 catcaaatga gcctcgtgga aatagatggt ccgtgattct gccctgtttt gcgtcgagtc
     2041 cgcgctttct cgcttctgcc acggcgtgtt ttgagatatc gagacccttc gcgctgtatc
     2101 ctgaagccct ggcctcgagt gcaaggccgc catatgcgca ccctacatcg agaaaatctg
     2161 caggcgccgg gacgaattct gcgatacgct tcaggcgagc tcgccagacg taggcttcgt
     2221 atttttcatt tttgcgttcg tctttgtagc tgtattcgct cttgccactg taatagtctt
     2281 cgtcgtacat ctgatcgatc tgtggcaaaa cgctttgcct ttcgagaccg caacggctgc
     2341 aacgaacaat gtcaaattca ggacggaagc gcttgatcgt ataacggtag atcataggcc
     2401 ctccacatag agggcacagt agttgctggc tagttgcttg catcgaagat ccagtggcag
     2461 acgcgatatt tgcttccaac cgggattctt tctacataac ccagcgaaac gtctgcgaaa
     2521 tagtccttca acagatccat tgcttccgga cgagtgtacg ttttgagatt cgccccgtcc
     2581 atgtcaggat tgtttttgaa atgggtatcg cgatgagatc ggagtgtgcc caggaatctt
     2641 ccgccctgct ttagaattcg catcacttct ttgagcatgc ttgcaatcat ttcgtccgga
     2701 ttgtagtgaa gcacacccca ggcgatcaca gtatccaggc tctcttctgg cagaggaatc
     2761 tcgaaatgat tcttcttgag catacgcgca tccagtggat gaaagagggc atagcgaaac
     2821 ctttttctgc acagttctag agcggtttcg cttacatctg tggcgtgaat ggcgctgtat
     2881 ccacaatctc gcaagaggga gatatgtctg ccgcttccgc agccaagatc taatccgctc
     2941 tgtcccggat gcttcttcag aagacgcacc aggttttcat ctggataatc ctgaacggat
//
LOCUS       DAORFF010000495         2995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_22060_length_2995_cov_3.821769, whole genome shotgun sequence.
ACCESSION   DAORFF010000495 DAORFF010000000
VERSION     DAORFF010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22060_length_2995_cov_3.821769"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2135
                     /locus_tag="PKK76_16295"
     CDS             <1..2135
                     /locus_tag="PKK76_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70092.1"
                     /translation="GFRTDAVMGNIRARYAAALDQAGQFFSVREFRRAAEQYRLASSL
                     AASMGEKGKYLEARLKSASALMTVDAPAADRAFAQLLAGELDAAQKSRVHAEGVKARF
                     RTGRFKEAEVAARVWAEAFPAEKSQIDQQIRLVVFLRNLESENASDKFHDGSFATEFR
                     ALLSDLAQPEVAPSAIDLLLRRGFHSEAASIARLQRPAERKAALARINFSQRLAEGKI
                     PQEAPRDVFDENEPGLAPLAAYRARVQSGEIISGVEIREDVNPTTLSPVMRSLLVQIY
                     LEASDSDPGYLIARLLREAIELEKGRSPERASLMALCAAERYLAFGDVEIADLFFNNS
                     RDLAKVAIADPERSRRLNNLQRILWWFSKQSAPEGAEEGLPSLEEEPMEAFLRSLDAS
                     QVPYGLRALKARALREHKYSLAVEINSAEQKGFERRGKIRPFVQKLQQNLPASQSVTV
                     ILESEMKIHRIRIGKDVDHDVWDKSARELRTRIARSVHMRERGDDADLARVFRAMVPA
                     PINRSTYVYLSSGFLLAPIYSEPGDRIFVVTDLERLARPAVPMDARFAGDFKVFIPGR
                     KALESADSSDRTWDERAARMEELAVGISGSTPGISHLMNPLALSSRIEGAWFSSVPRH
                     GDSALQTVVKYENDLIQALHSASAPGVFYAETGASEWAVRFIRFYYDRESGMTMVDRR
                     YMDAQIRLRREGMGPLNVRLATPVLISD"
     gene            complement(2132..>2995)
                     /locus_tag="PKK76_16300"
     CDS             complement(2132..>2995)
                     /locus_tag="PKK76_16300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HNL70093.1"
                     /translation="IWSAVWIALALAFNALFYFYSSWKFAQIGLADAEGEARRVALEF
                     LSGYLIEKSLSIDNIFVFILIFGYFAIPQKYQHRVLFFGIIGALVFRAAFVAIGAALM
                     QFHWVIYLFGGFLILTGLKMAFAQEKEMDLSDNLAMKLFKKILPATHDLHGSKFLVKI
                     NGIRHATPLLLALIFVEISDIIFAVDSVPAIFAITKEPLIVFTSNVFAILGLRSLFFL
                     LAGSSDKFHLLKYGLAVVLVFVGLKMVWLNDAFGGKFPISWSLGIILGTIALFMLLSV
                     LFPEKKKANSG"
BASE COUNT          745 a          726 c          831 g          693 t
ORIGIN      
        1 agggctttcg cacggatgct gtgatgggaa atatccgtgc gcgctatgca gccgctctcg
       61 atcaagcagg tcagttcttt tccgtgcgcg agttcaggcg cgccgccgag cagtacaggc
      121 ttgcttcttc cctggcagct tctatgggcg aaaaaggcaa gtaccttgag gccagattga
      181 agtctgcatc cgcactcatg acggtcgatg cgcccgcggc tgatcgcgct ttcgcgcaac
      241 ttctggcggg ggaactggac gccgcacaaa agtcgagggt tcacgcagaa ggagtgaagg
      301 cgcgttttcg gactggacga tttaaggaag cggaagtagc cgcacgagtc tgggcggaag
      361 catttccggc ggagaaaagt cagattgatc aacagatccg tctggttgtg ttcttgcgaa
      421 atctggagag cgaaaacgct tcggacaaat ttcatgatgg atcatttgcc acagaatttc
      481 gtgcgttgct ttctgatctc gctcaaccag aggttgcacc ctctgcgatt gatttgttgc
      541 tcaggcgcgg ttttcactca gaagcagctt ccattgccag attgcagaga cctgcagaaa
      601 ggaaggccgc acttgcgcga atcaacttca gtcaaaggct cgcggaagga aaaatacctc
      661 aggaagcacc ccgtgacgtt ttcgacgaaa acgaaccagg tctcgctcct cttgctgctt
      721 accgggccag agtgcagagc ggggagatta tttctggcgt agagattcgt gaggatgtaa
      781 atccaaccac cttatcaccc gtaatgcgat ctttgctcgt tcagatatac ctggaagctt
      841 ctgattcaga tcccggctat ttgatagctc ggttgttgcg cgaagcgatt gagctggaga
      901 aaggcaggtc cccggagaga gcatccctga tggctctgtg cgccgccgaa cgttatctgg
      961 cattcggaga cgttgaaatc gcggatctct tctttaacaa ttcacgtgat ctggcgaaag
     1021 tagcgatagc agacccggaa cgatcgagac gtctcaacaa tttgcaacga atcctgtggt
     1081 ggttctcaaa gcagtcagcc ccggaaggag ctgaagaagg tcttccatct ttagaggaag
     1141 agccgatgga ggcgttcctt cgttctctgg atgcttcgca agttccatac ggattgcggg
     1201 cacttaaagc gcgggcgttg cgagaacaca agtattctct ggccgtggaa atcaattctg
     1261 cggagcagaa gggttttgaa aggcgaggta agatccgtcc atttgtccag aagcttcagc
     1321 agaatcttcc agcttctcag agcgtgactg ttatccttga atcggaaatg aagattcata
     1381 gaattcgcat cggcaaagac gtggatcatg acgtatggga caaaagcgct cgcgaactgc
     1441 gcactcgcat tgcgcgttcc gtgcatatgc gtgaacgtgg agacgatgcg gatcttgctc
     1501 gagttttcag agcaatggta ccggcgccca tcaatcggag cacctacgtc tatttgagtt
     1561 ctggttttct tctggcccct atctattctg agcccggaga tcgcatcttt gtagtcacgg
     1621 atttggagcg actcgctcgc cctgctgtgc ccatggatgc gcgttttgcc ggcgatttca
     1681 aagtattcat tccaggcagg aaagcactgg agagcgccga ttcaagtgac agaacgtggg
     1741 acgagagagc tgcgcgaatg gaggaactgg ctgtaggcat ttcaggaagc actcctggaa
     1801 tttcgcactt gatgaatcca ctcgcgctat cgagtcggat cgagggcgcg tggttttcga
     1861 gcgtgccgcg tcatggcgat tctgcgttac aaacagtcgt gaaatacgaa aacgatttga
     1921 ttcaggcact ccattcagct tctgctccag gtgtgtttta cgcggaaacg ggagcatctg
     1981 aatgggcagt gcgcttcatt cggttctact atgatcgaga gtccggtatg accatggtcg
     2041 atcgcagata tatggatgcg cagattcgac ttcgtcgtga aggaatgggg ccgctgaatg
     2101 tgcggctggc cactcctgtc ttgatttccg actaaccgct gttagctttt ttcttttctg
     2161 gaaacagaac ggaaagaagc atgaacaggg caatggttcc cagtatgatt cccagagacc
     2221 acgaaatcgg gaattttccg ccgaacgcgt cattgagcca caccattttc aatccgacga
     2281 agactagaac cacggccagg ccgtacttta gcagatggaa tttgtcggaa gatccagcga
     2341 gcaagaagaa gagagatcgc aggcccagaa tggcgaatac attcgaagtg aatacgatga
     2401 gcggttcctt cgtaatggcg aagatggccg gcacgctatc cactgcgaag atgatatccg
     2461 agatctctac aaagatgagt gccagaagaa gcggagttgc atgacgaatc ccgttgatct
     2521 tgaccaggaa ttttgaccca tgcagatcat gggtggcagg caggatcttt ttgaaaagct
     2581 tcatggccag gttatcggag aggtccattt ccttttcctg cgcgaaagcc atcttcaggc
     2641 cagtaagaat caagaatcca ccgaacaggt agatgaccca gtgaaactgc atgagagcag
     2701 cgccgatggc cacaaatgcc gcgcgaaaca caagtgctcc tatgattcca aaaaagagaa
     2761 cgcgatgctg gtacttctga ggaattgcga aatagccaaa gatcaagatg aagacgaaga
     2821 tgttgtcaat ggaaagagac ttttcgatca ggtagccact gaggaattct agcgccactc
     2881 tgcgcgcttc tccctctgcg tccgccagtc caatctgcgc gaatttccac gaagaataga
     2941 aatagaagag cgcattgaat gctagcgcca gtgcgatcca caccgcactc cagat
//
LOCUS       DAORFF010000496         2981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_22264_length_2981_cov_3.496582, whole genome shotgun sequence.
ACCESSION   DAORFF010000496 DAORFF010000000
VERSION     DAORFF010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22264_length_2981_cov_3.496582"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKK76_16305"
     CDS             complement(<1..255)
                     /locus_tag="PKK76_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70094.1"
                     /translation="MKLDRYVVIKENSGGHNAVAFCDVDSYVYLNPSASSSCTIEEIS
                     HEIASEEGEPGESVRARIAACNPGLDVAKAAFQAGSLIRVK"
     gene            408..2276
                     /locus_tag="PKK76_16310"
     CDS             408..2276
                     /locus_tag="PKK76_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNL70095.1"
                     /translation="METTAVKRQLEIKETDSVVIRFSGDSGDGMQLTGMQFTDTTAIL
                     GNDLSTFPEFPAEIRAPAGTLAGVSGFQVHFGSKEIFTPGDKYDVLVAMNAAALKADL
                     KNLREHGVIIVNTAGFDERNLKLAQYPEGAHPLDDGSLDHYRLYKLDISRLTKDSMDG
                     LEVSAKDVDRAKNMFVLGLLFWMFERSVSNTEKFIQEKFSKKPLIQDANIRALRAGFN
                     YGETAEIFTTRYHVAKAKMPPGVYRNITGNQALAIGLIAAGEKSGLPVFLGSYPITPA
                     SDILHELSRYKANGVRTFQAEDEISAITSAIGASYGGALAVTTTAGPGLALKTEAMGF
                     ATMLEIPLVIIDVQRGGPSTGLPTRTEQADLMMAMFGRHGEAPLPIVAVATPADCFET
                     IYEACRITLEHMTPVIVLSDGYIANGSEPWKFPAESDLKSITVHFTTENNNPSGGTFL
                     SYKRNDQLVRPWVKPGTRGLEHRIGGLEKQHETGNVSYDPANHERMVKIRAEKVEKIA
                     DSIPPAKVDSGREGAKVCVLGWGSTYGSIKGAVTELIAEGHDVAHIHLRHLNPFPKNL
                     GDILRGFGKVLIPEINNGQLVQLIRAKYLIPAVPLNKIMGLPFATTEIKSTIKEML"
     gene            2273..>2981
                     /locus_tag="PKK76_16315"
     CDS             2273..>2981
                     /locus_tag="PKK76_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNL70096.1"
                     /translation="MSAPLSTEKLTPKDFASSQEVKWCPGCGDYSILKQVQTVFPELE
                     VEREKFVIVSGIGCSSRFPYYMETYGMHSIHGRAFAVASGLKATNPDLHVWVVTGDGD
                     ALSIGGNHFIHLMRRNFDIKVMMFNNQIYGLTKGQYSPTSEKGKQTKSTPYGSIDEPF
                     NPAELALGAKASFVARTLDRDPKHMQAMIRRSHEHKGTSFLEIYQNCPVFNDAAFFPF
                     TDRETRKEESLFVEDGMP"
BASE COUNT          783 a          757 c          724 g          717 t
ORIGIN      
        1 cttgacgcga atcaaggagc ctgcctgaaa cgcagccttc gctacatcga gacctggatt
       61 gcacgctgca attctcgcgc gcacactttc tcctggttcg ccctcctcac ttgcaatttc
      121 gtgagagatc tcttcgatcg tgcaactgga ggaggccgac ggattcaaat agacataaga
      181 atcaacatca caaaacgcaa ctgcgttgtg tcctccagag ttttctttga taaccacgta
      241 acgatcgagc ttcatagctt cccttaccag gcggaatgat cctgcccatc ttttttgcgt
      301 ggaaaacggc tggatctgac gttttgacgc aggtttatag ccctatttgt agtcgacact
      361 ggtctgcccg agctggacct tggactcaac ggtaattcaa accaacaatg gaaacaactg
      421 cagtcaagcg tcagcttgaa atcaaagaaa cagatagcgt agtcattcga ttttcgggag
      481 attccggcga cggaatgcag ctcaccggaa tgcaattcac agacacaaca gccattctag
      541 gaaacgactt aagtacgttc ccggaatttc cggctgaaat tcgcgcaccg gcgggcactc
      601 tggccggcgt atccggattt caggttcatt tcggaagcaa agagattttc actccaggcg
      661 acaaatacga tgtgctcgtt gccatgaacg ccgcagcact caaagcggat ctcaagaatc
      721 tgcgcgaaca cggagtgatc attgtaaaca ctgcaggctt tgatgagcgc aacctaaagc
      781 tcgcacaata ccccgaaggt gctcatccac tcgatgacgg cagcctggat cactatcgtc
      841 tgtacaagct ggacatatca cgccttacaa aggatagcat ggatggcctg gaagtatctg
      901 cgaaggacgt ggaccgagcg aagaacatgt tcgttcttgg tttgcttttc tggatgttcg
      961 aaagatccgt ctcaaatact gagaaattca ttcaagagaa attttcaaag aagcctttga
     1021 ttcaggacgc gaacattcgc gcacttcgcg ccggtttcaa ctatggtgaa acggcggaaa
     1081 ttttcacaac gcgttaccat gtcgcaaaag cgaagatgcc tcccggtgtt tatcgcaaca
     1141 ttacggggaa tcaggctctg gccattggtt tgattgctgc cggggaaaaa tccggtctgc
     1201 cagttttcct tggttcctat cccatcactc ctgcatcgga cattctacac gagctttctc
     1261 gctacaaagc gaatggagtg cgcacgttcc aggcggaaga tgaaatttct gccatcacaa
     1321 gtgcaatcgg agcttcgtat ggcggtgcgc ttgcagttac aaccactgcg ggtcctggtc
     1381 tcgctctaaa aacagaagca atgggttttg caaccatgct tgaaattccg ctggtcatca
     1441 ttgatgtgca gcgcgggggc ccgtctacag gtcttcccac tcgcacagaa caggcggatt
     1501 tgatgatggc aatgttcgga agacacggag aagctcctct tcccatcgtt gctgtggcca
     1561 ctcctgcaga ctgtttcgaa acaatttatg aagcatgccg cataacgttg gagcacatga
     1621 ctccagtgat tgttctaagt gacggataca ttgcaaacgg ttctgagccg tggaagtttc
     1681 ctgctgaatc tgatctcaag tcaattacgg ttcatttcac aacggaaaac aacaatccat
     1741 ccggtgggac tttcctttcc tacaagcgaa acgatcagct ggttcgtccg tgggtgaagc
     1801 cagggaccag gggcctcgag catcgcatcg gtggtctgga aaagcagcat gaaactggaa
     1861 acgtttctta cgatccagca aaccacgaaa gaatggtgaa aattcgcgct gaaaaagtag
     1921 aaaagatcgc ggacagcatt ccacctgcga aagtggattc aggccgcgaa ggcgcgaaag
     1981 tgtgtgttct cggatgggga tctacctacg gctccatcaa gggcgctgtg acagagctga
     2041 tcgcagaagg tcatgacgtg gctcacattc accttcgcca cctcaatcca tttccaaaga
     2101 atctggggga tatactccgc ggttttggaa aagtactcat tcctgaaatc aacaacgggc
     2161 agctggtcca gttgatccgt gcaaagtatc tgatcccggc cgtgcctcta aacaaaatca
     2221 tggggctccc cttcgctacg acagaaatca aatctaccat caaggagatg ctatgagcgc
     2281 tcccctctct acggagaaac tcactccaaa ggacttcgct tccagtcagg aagtaaaatg
     2341 gtgccccggc tgcggggact attccattct aaaacaagtg caaacagttt tcccggaact
     2401 ggaagtggaa cgggaaaagt ttgtcattgt ttctgggatt ggctgttcct ctcgctttcc
     2461 gtattacatg gaaacttatg gaatgcatag cattcacgga cgtgccttcg ctgttgcttc
     2521 cggactcaag gcaaccaacc ctgatttgca tgtttgggtg gtgactggag acggagatgc
     2581 tctttctatt ggcggaaatc acttcattca cctgatgcgt cgtaactttg acatcaaagt
     2641 catgatgttc aacaatcaga tctatgggct taccaagggt cagtattcgc caacttctga
     2701 aaaaggaaaa cagacaaagt ctactcctta cggatccata gacgaacctt tcaatccggc
     2761 agaattggcg ctgggtgcca aagcttcgtt tgtagcgcgc acactggacc gcgatcccaa
     2821 gcatatgcaa gcgatgattc gcagatcgca cgaacacaaa ggcacaagct ttcttgaaat
     2881 ctatcagaat tgcccggtat tcaatgatgc cgctttcttt ccctttacgg accgcgaaac
     2941 gcgcaaagaa gaatctctct tcgtggaaga tggaatgcca t
//
LOCUS       DAORFF010000497         2980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_22278_length_2980_cov_2.821880, whole genome shotgun sequence.
ACCESSION   DAORFF010000497 DAORFF010000000
VERSION     DAORFF010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22278_length_2980_cov_2.821880"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PKK76_16320"
     CDS             <1..670
                     /locus_tag="PKK76_16320"
                     /inference="COORDINATES: protein motif:HMM:NF013151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNL70097.1"
                     /translation="VRELGRLLRLPEKLVDRHPFPGPGLAIRLLCSDADNADFRSAAA
                     LLAEHNQPLLKDIECTILPIKSVGVQGDQRTYAHPALVYEREGDTARLHEIARALPNF
                     VREVNRVLRFVAVRKAELSADRVERLREADHVVNSFLHEKNIYGEIWQFPVVLVPLAR
                     KGVEEDAVILRPVVSLDAMTVSPYEIKRDQLKDLSNRLLAISGIGSILYDLTTKPPGT
                     IEWE"
     gene            complement(932..1783)
                     /gene="speE"
                     /locus_tag="PKK76_16325"
     CDS             complement(932..1783)
                     /gene="speE"
                     /locus_tag="PKK76_16325"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNL70098.1"
                     /translation="MAEWFTEQLAIVPGMRAQIEVTKKLASERSQFQKLEVYETTSLG
                     RMLVLDDVIQCTEFDEQSYHEMICQVPLFAHPNPQKVLIIGGGDGGSVRECLKHKSVQ
                     EVHLCEIDKRVVDLCLEHIPSMAGKVTDPRVHCHYEDGAAWAKNHPDTYDIIIVDSSD
                     PIGPAEVLFQFPFYESCRNALKSDGILATQAENFLLHAKIIKELLSFGKKLFPISRYY
                     YTQVPTYPGGMIGFTFFSRKHAHDELVDKRFAESGIKAEEFRFWSVDFHKKAFTLPGA
                     TMRDFYS"
     gene            1852..2325
                     /locus_tag="PKK76_16330"
     CDS             1852..2325
                     /locus_tag="PKK76_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNL70099.1"
                     /translation="MSEKPFRILGIQQVAIGGPDKGALKKLWVDMFGLSIAGTFKSEK
                     ENVDEDILRLGRGPLAVEVDVMQPIDPEKSPKVHVPPLNHIGLWVENLEAAVKWLESS
                     GVRFAPGGIRKGAAGYNVCFIHPKGNEQFPIGGEGVLIELVEAPAEVSAAFDKLS"
BASE COUNT          716 a          724 c          807 g          733 t
ORIGIN      
        1 ggtaagggag ctgggtcgat tgctccggtt gccagagaaa ctcgtggatc ggcatccatt
       61 tccagggccc gggcttgcga ttcgcctttt gtgttctgac gcggacaatg cagactttcg
      121 ttctgcagcg gctttactcg ctgagcacaa tcagccattg ctcaaggaca ttgagtgcac
      181 cattcttccg atcaaatcag tgggagtcca gggggaccag agaacctatg ctcacccggc
      241 tctcgtatac gagcgcgaag gtgacactgc gcgcctgcat gagatcgctc gtgcgcttcc
      301 gaattttgtt cgtgaggtca atcgtgttct tcgctttgtg gcggttcgca aggctgaatt
      361 atctgcggat cgggtggaaa ggctgcgcga agcagatcac gttgtgaatt cgtttctaca
      421 cgaaaaaaac atctacggtg aaatctggca gtttcctgtt gtgcttgttc ctctggcgcg
      481 aaagggagtg gaagaggatg ctgtcattct tcgcccggtc gtgtctctgg atgcgatgac
      541 tgtttctccc tacgagatca aacgggacca gttgaaagat ctttccaatc gccttcttgc
      601 aatctccggg attggttcca ttctttacga cctgaccaca aagccacctg gcacgatcga
      661 gtgggaatga cgcgattttg aaattctcag tgcgccgcgg gccggagtgt atcccaaccg
      721 cagcgagacg agcctccatg cccgactcgc agcggcgctc cgccatcgtg gcttcgcaat
      781 tgggacacac cccgcccggg cgggttcgac tttgcaaaat cactctcagc aacattgcaa
      841 ccatcggaga tataaaatct cttccagatc gacctgcctg ccgactcggc tttgcaaaat
      901 cactcccaga tcagcaacca gctgaagtga atcaggagta gaaatcgcgc atggtagcac
      961 ccggcagagt gaacgctttt ttgtgaaagt caacggacca gaagcggaat tcttctgcct
     1021 tgatccctga ctctgcgaag cgtttgtcta caagttcatc gtgtgcgtgc ttgcgagaaa
     1081 agaaagtaaa tccaatcatc ccgcccgggt aggtgggaac ctgggtgtag tagtatctgg
     1141 aaattggaaa tagtttctta ccaaacgaaa gaagttcttt gatgatcttt gcatgaagca
     1201 gaaaattttc tgcctgcgtt gccaggattc catctgactt cagtgcgttt cggcagcttt
     1261 cgtagaatgg aaattgaaac agcacttctg ccgggccgat tggatcggat gaatccacaa
     1321 tgatgatgtc gtatgtgtcc ggatgattct ttgcccaggc agcgccgtct tcatagtggc
     1381 agtgcacgcg agggtctgtc accttgcccg ccatggaagg aatgtgttcc aggcatagat
     1441 ccactacgcg tttgtctatt tcgcacagat ggacctcctg aacgcttttg tgtttgaggc
     1501 attctcgtac agatccgccg tctccgcctc caatgatcag aactttttga ggattcggat
     1561 gtgcgaagag tggaacctgg cagatcatct catgatagga ctgctcatcg aattctgtgc
     1621 actggattac atcgtccagg acaagcattc tgcccaggga agtggtttcg tatacttcca
     1681 gcttctgaaa ctgagagcgt tcgcttgcca gtttctttgt aacttcgatt tgagcgcgca
     1741 ttcctggaac gattgcgagc tgctctgtaa accattctgc catggaactc tcccgggtcc
     1801 aactccgtaa atggagtgaa aaaaattcaa agccacaaaa caatgccacg catgagtgaa
     1861 aagccatttc gaattcttgg aatccagcag gtcgccattg gcggcccgga caaaggcgcg
     1921 ttgaagaaac tgtgggtgga tatgtttggc ctgagcatcg caggcacgtt caaaagcgaa
     1981 aaagaaaatg tggatgaaga catccttcgc ctgggccggg gccctcttgc agtggaagta
     2041 gatgtgatgc agcccatcga cccggaaaaa tcgccaaagg tgcacgttcc gcctctcaat
     2101 cacattggtc tgtgggtgga gaatctggaa gcggcagtga agtggctcga atcgagtgga
     2161 gtgcgttttg ctccaggcgg gattcgcaag ggcgctgccg gatacaatgt ttgctttatt
     2221 catccaaaag gaaacgagca gtttccaatc ggcggagagg gagttctcat tgaacttgtg
     2281 gaagcacctg cagaagtttc ggcggcattt gataagttga gctaatcagg acgcgaaatg
     2341 tggcgatttt gaaccgcgca tcaaacccga gggaaaggat cgattcgctg cgcgaatcgt
     2401 ttagaaaaat gcgcgcctca aattgtgaaa cccagaaggt atgcggatac agtagttgcc
     2461 catgagaatg aattcaaacg acgaccttta ttgcacaact tgtgggcaac ttcttggaat
     2521 aaaaacggac gagcatggga atgaattctt gatttgttgc ccggcgctcc ttgctgagaa
     2581 aacagggcac acgattctca tcacggaaac gcgtgcctct tcctcgctgt gcgcaaacgc
     2641 ttgcgggaat tggggtcgaa accgattcta atatcttctg atgaatatgt tggaacaggg
     2701 tatgggcgcc ccgacgacag cgaaggactg taggcgccgc cacctggatt tgttgcactc
     2761 aaaaccactc gctaagaagt tgaacgttat tcgccattga tcagggccag attaaacgaa
     2821 cgtgagagcg gcgtttttga ttcgtctatc aaacctgagg ggaaagttcg attcgctgcg
     2881 cgaatcgttt agaatttacg ccgcttttta agtgaaagcc tcgaaggccc gcgagattgt
     2941 gatatcccgc gtgtgcggtc cacagcggat ttcggagcgt
//
LOCUS       DAORFF010000498         2977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_22313_length_2977_cov_4.390144, whole genome shotgun sequence.
ACCESSION   DAORFF010000498 DAORFF010000000
VERSION     DAORFF010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22313_length_2977_cov_4.390144"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PKK76_16335"
     CDS             complement(<1..220)
                     /locus_tag="PKK76_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70100.1"
                     /translation="MNRKKYLFPVLLAVSTGQCITSHYHDETVDYANPRHEFKSGKKT
                     IPGHAVSRTDGSTDIYFADILEGKERLLK"
     gene            complement(228..1193)
                     /locus_tag="PKK76_16340"
     CDS             complement(228..1193)
                     /locus_tag="PKK76_16340"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNL70101.1"
                     /translation="MFESVKKWLRGRPGPRRLMILNPTSAKGQALQNFDRIQVRLREM
                     LGTYDLKFTRGPGDATEMVRRALKEREYRQILVAGGDGTIHEAMNGYFEKGRSLSKDI
                     PLGVINLGTGGDFYKTLRAMSPDYAAALMRNQFRLVDCGEASIEPMGYTRYFMNIASA
                     GIAGQVLKNLKASRFQAGTAAFFYHTLSTLVKYTPARVKITFTDTDGRHHDFEERIIN
                     FFACNGKFNGGGMKWAPFGNLDDGAFEIVIIRRTSKLRMILDSRKVYTGHVEGMEGVS
                     QFETKEILLQPIDPITFELDGEVPPIASPKSIRFRNIHAQLPVVL"
     gene            complement(1186..>2977)
                     /gene="mnmG"
                     /locus_tag="PKK76_16345"
     CDS             complement(1186..>2977)
                     /gene="mnmG"
                     /locus_tag="PKK76_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNL70102.1"
                     /translation="LLTMNLDTIGQMSCNPAIGGIAKGHMVREVDALGGIMGRVIDQT
                     GIHFKMLNRSRGPAVWAPRAQAEKKAYQNHIKWTLEATPNLSFRQDTCDSLIIENHKV
                     CGIICSRGQEIQCDYVILTTGTFLQGLIHIGEYQARSGRIAEQSAMKLSDCLTAYGFE
                     LGRLKTGTPPRVLKKSLDLSVMEEQEPDPVPSPFSYSTSAITQPQISCYVTYTGAPTH
                     EIIRQNLDRSPMYSGQIKSTGPRYCPSIEDKIVRFADKERHQVFIEPEGVETGEVYLN
                     GVSTSLPEEVQWQVVRSIRGMENAEIIRPGYAVEYDFVDPRELHHSFETRKIQNLFFA
                     GQINGTTGYEEAAAQGMIAGINVLRKSRNESPFILGRGEAYIGVLVDDLVLKGVEDPY
                     RMFTSRAEHRLLLRQDNADERLMKYGFELGLLGEDSYRAMLDRGQRIKKMRDLISTRG
                     IRATQEVADLFAKKNMEMPKSGFGKTLDSLLRRPEVSIEDLTVIIPELTELTRDERSV
                     LELEIKYAGYVKRELEKVDRRERISNHPIPANFDFDTVPGIKKEAREKLKRFRPHDLN
                     TASRISGVDPPDIDLIYLKILEAEKAQSHV"
BASE COUNT          659 a          729 c          709 g          880 t
ORIGIN      
        1 tcttcaaaag tctttctttg ccttccagaa tgtctgcgaa atagatatcc gtggagccgt
       61 cggtcctgga gacagcgtgg ccagggatgg tctttttccc gcttttgaac tcgtgacggg
      121 gattcgcgta gtccaccgtt tcgtcatggt agtgacttgt gatgcattgt ccggttgaga
      181 ctgccagtag aacaggaaaa aggtatttct ttcgattcat ctttcctcta taagacgaca
      241 ggaagttgcg catgtatgtt tcggaaccta atggatttag gagatgcgat gggaggaact
      301 tcgccatcga gttcaaaagt gatcgggtcg atgggctgga gtagaatttc ctttgtctca
      361 aactgagaaa cgccttccat cccttccacg tgtcccgtgt agacttttct actgtccaga
      421 atcatgcgaa gcttgcttgt gcgacggatg attacgattt caaaagctcc atcatccaga
      481 tttccaaacg gggcccattt cattcctccg ccattgaatt ttccattgca cgcgaagaag
      541 ttgatgatgc gttcttcaaa atcgtgatgt cttccgtctg tgtctgtgaa agtgattttt
      601 acccgggccg gagtatactt gacgagagtg ctcagtgtgt ggtagaaaaa tgcagccgtt
      661 cctgcctgaa atctggatgc tttcaaattc ttcaatacct gacctgcgat tccggcagat
      721 gcgatattca tgaaatagcg agtgtagccc atgggttcga tcgacgcttc tccgcaatcc
      781 accagacgaa actgatttcg catgagagct gcggcgtaat cagggctcat cgcacggaga
      841 gttttgtaga agtctcctcc ggtgcccagg ttgatcacgc cgagtggaat gtctttgctc
      901 aagcttcggc ctttttcaaa gtatccattc atggcctcgt ggatggttcc gtcaccgccc
      961 gcgaccagga tttgtctgta ttcgcgttct tttaacgcgc gacgaaccat ctccgttgca
     1021 tccccggggc ctcgagtgaa tttcagatca taggtgccca gcatttcgcg caatctgacc
     1081 tggattctgt cgaagttctg gagagcctga cctttcgcgc tggttggatt gagaatcatc
     1141 aaccgacgtg gtccgggtct accacgcagc cattttttga cagattcaaa catgcgattg
     1201 ggccttttct gcttctaaaa ttttcaaata gatcaaatcg atatccggtg ggtccacacc
     1261 ggaaatacgc gaggcggtat ttagatcgtg aggtctaaat cgttttagtt tttcgcgcgc
     1321 ttctttcttg attccaggga ctgtatcaaa atcgaaattc gcgggaattg gatggttgga
     1381 tattctttcg cgtctgtcta ctttttcgag ttcgcgtttc acatatccag catacttgat
     1441 ttccaattct agaacacttc gctcgtcgcg cgtaagttca gtgagctctg ggataattac
     1501 tgttagatct tcaatgctga cttctggcct gcgcaaaagt gaatcgagtg ttttaccaaa
     1561 tcctgacttt ggcatttcca tgttcttttt ggcaaacaga tccgcgactt cctgtgtggc
     1621 gcgaattccc cgagtcgaaa tcagatcgcg catctttttg attctttgtc cacgatcgag
     1681 catagctctg tacgaatctt ctcccagcag tcccagctca aacccatatt tcatgagtcg
     1741 ttcgtctgca ttgtcctgtc tgagaagaag tctgtgttcc gcgcggctgg tgaacattct
     1801 gtagggatct tctaccccct tgagaaccag atcgtctacg agaacaccaa tgtatgcttc
     1861 tccgcgtccc aggatgaagg gagattcatt tcttgatttg cgaagcacat tgattccagc
     1921 gatcattccc tgcgcggctg cctcttcata tccggtggtt ccgttgatct gcccggcaaa
     1981 aaacaaattc tgaattttgc gcgtctcgaa agaatgatgc aactctcgcg gatcgacgaa
     2041 atcgtattca acagcatatc cagggcgaat gatttccgcg ttttccatgc cgcgaatgct
     2101 tcgcacgact tgccattgta cttcttccgg tagacttgta cttacgccat taagataaac
     2161 ttctcctgtc tccacacctt ctggttcaat gaatacctgg tggcgctctt tgtctgcgaa
     2221 tcgtacaatt ttgtcttcaa tcgaagggca ataacgtggc cctgtacttt tgatctgtcc
     2281 cgaatacatg ggacttctgt ctagattttg ccggatgatt tcgtgtgtcg gtgcccccgt
     2341 atatgtaaca taacaggaaa tctgtggttg cgtgatggcg gatgtgctgt aggaaaaagg
     2401 agaaggtact ggatctggtt cttgctcttc catcacactg aggtccagag attttttcaa
     2461 cacgcgcgga ggagttccag ttttcagtct gcctagttca aatccgtacg cagtcagaca
     2521 gtcagagagc ttcatggccg attgttccgc aattctcccg gatcttgcct gatactctcc
     2581 aatgtgaatg agtccctgaa gaaaagttcc tgtagtcaga atcacatagt cgcattgtat
     2641 ttcctggccg cgactgcaga tgatcccaca aactttatgg ttctcaatga taagtgaatc
     2701 acaagtatcc tggcggaagc ttagattggg agttgcttcc agggtccact tgatatggtt
     2761 ttgataggct tttttttctg cctgtgcccg cggtgcccac actgcgggtc ctcgtgaacg
     2821 attcagcatt ttgaaatgga ttccagtctg gtcgatcaca cggcccatga ttccaccaag
     2881 cgcgtccact tcgcgcacca tgtgtccttt cgcgattcct ccgatggcag gattgcaaga
     2941 catctgaccg attgtgtcca gattcatggt cagaaga
//
LOCUS       DAORFF010000499         2974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_22349_length_2974_cov_3.460432, whole genome shotgun sequence.
ACCESSION   DAORFF010000499 DAORFF010000000
VERSION     DAORFF010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22349_length_2974_cov_3.460432"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PKK76_16350"
     CDS             <1..591
                     /locus_tag="PKK76_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70103.1"
                     /translation="VLDYNPNTRTSTSFAAIPGYGEAVLATNGKFYGVPNNTNPPVGE
                     IDPIARSLRTGGGTYGVGAYDGGSIAPNGKVYMTPLAATTVLEVDPDLFPASNAFSQF
                     GSTFGNQYSNGILAPNGKIYAPPRTGTSVLVTDPLNRTTSTIGTTSGVYIGATMGLNG
                     KIYFIPNGATTIAELDTGSAGTFCPNVILSSYINSN"
     gene            complement(588..1676)
                     /locus_tag="PKK76_16355"
     CDS             complement(588..1676)
                     /locus_tag="PKK76_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70104.1"
                     /translation="MDALWKDLSELLTAFGEIQDRLDPTLFSQSASRIEPLAAKLELS
                     RNEAERSTQNAPSVDVLLRSASQALQGAARFLEAPAGPHGIMKAFQALRPIHRSEEIL
                     YSLAGQFEEVSKFFLPASHRENRELLKRLSQSPSGDIASLSDASDCGIIHFNNERGKR
                     GGWSIYVPEYYTKEKRWPLVIALHGGSGHGADFLWSWLRDARARGMLLASPTSLDRTW
                     FLHHPGTDVVGLNSMLSQISEKWNVDTKRLLLTGISDGGTYSMVLSTVRQSPFTHYAP
                     VAAAVHALLNREGIVEAPVADLKIFQVHGARDWMFPVEKARLAASALKNAGANIVYRE
                     VEDLSHNYPRDLNAEILDWFTRSGSDLY"
     gene            1987..2145
                     /locus_tag="PKK76_16360"
     CDS             1987..2145
                     /locus_tag="PKK76_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70105.1"
                     /translation="MKQGARFVDQGRIITSAGISAGIDMSLHIVERYFGKEASRATAR
                     RMEYDFIS"
     gene            complement(2165..>2974)
                     /locus_tag="PKK76_16365"
     CDS             complement(2165..>2974)
                     /locus_tag="PKK76_16365"
                     /inference="COORDINATES: protein motif:HMM:NF015776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HNL70106.1"
                     /translation="RDGLLAHAHRQPDFVKMDGDVVWSNDGFETSMINGERSIVHTVK
                     NPAERGHIIGAWAMVERRDHGITYFYAPLGEYKRNTPIWNSHPSAMILKVAESYALRK
                     AYSISGVVGEAEVEASNPATNITAIPGTARDLHPDWGDDPVLAQDLKATTAFDCISGA
                     MSARVLQALPSRSQIIVYGSMSGEDATAKSKDIVFFRKNVTGFHLASHIKERGTLWVL
                     KTARQVQKLVERKIFHTEFSARLTLAQAPEGIAAYAENMTSGKPVILPRGV"
BASE COUNT          665 a          766 c          806 g          737 t
ORIGIN      
        1 gttctggatt acaatccgaa tactagaact tccacaagct ttgcggccat ccccgggtat
       61 ggagaagctg tgcttgcaac caacggtaaa ttttacggag ttccaaacaa cacgaatccc
      121 cctgtgggag aaatcgatcc cattgccagg tctcttcgaa ccggtggagg cacgtatggt
      181 gtaggcgctt atgacggagg atctattgcg cctaacggta aggtctacat gacacctctg
      241 gctgcaacca ctgttctgga agtggatccg gatctatttc cagcatcgaa tgcattcagt
      301 cagtttggca gcacatttgg aaaccagtat tccaatggaa ttctggcacc gaatggaaag
      361 atctatgctc cgccgcggac aggcacttct gttctggtta cagatccgtt gaatagaact
      421 acctctacga tcggcactac ttcgggggtt tacattggtg ccaccatggg cttgaacgga
      481 aagatctact tcattccaaa cggagccacc accatcgctg aactggacac aggttcggct
      541 ggcacctttt gtccgaatgt gattttatct tcttatataa attccaacta gtatagatca
      601 ctcccgctgc gcgtaaacca gtccagaatt tccgcattca ggtcgcgtgg atagttgtgc
      661 gaaaggtctt ccacttcgcg gtagacgatg tttgctcctg cattcttgag cgctgatgcg
      721 gcgagtcttg ctttttccac agggaacatc cagtcgcgcg ctccatgcac ctgaaagatt
      781 ttcaggtctg cgaccggcgc ttcgacgatt ccttctctgt tcaaaagagc gtgtactgct
      841 gcggctacag gagcatagtg ggtgaaagga gattgtctga cagtggagag gaccatggag
      901 tatgttccgc cgtctgaaat ccccgtgagc aagaggcgct ttgtatccac gttccatttt
      961 tctgagatct gactcagcat ggaattcagg cccactacat ccgtgccagg atggtggaga
     1021 aaccaggtgc gatccagaga tgtaggggac gcaaggagca tcccgcgtgc gcgcgcatct
     1081 cgcagccagc tccacaggaa atcagctccg tgaccggatc caccgtgcag tgcaatgacc
     1141 agaggccaac gtttttcttt tgtatagtat tctggcacat agatagacca tccgcctctc
     1201 tttccgcgtt cgttgttgaa atggatgatt ccacagtcac tcgcatccga aagtgacgca
     1261 atgtcgcctg acggtgactg agacaacctt ttcagcagct ctcgattttc tctgtgcgat
     1321 gctggaagaa agaatttgct cacttcttca aattgacccg ccagcgaata caggatttcc
     1381 tccgagcgat ggatgggtct aagcgcctgg aatgccttca tgattccgtg cgggcctgcg
     1441 ggagcttcca ggaatcgagc ggctccctgc aatgcctggg aagcggaacg caacaatacg
     1501 tctacggatg gagcgttctg ggtgctacgc tctgcttcat ttctagaaag ttccagtttt
     1561 gcggccagtg gttcaattct gcttgcagat tgtgaaaaga gggttggatc cagcctgtcc
     1621 tggatttcgc caaaagctgt gagcagttcc gaaaggtctt tccacaaagc atccattggt
     1681 tttggatttc gacgagctct gctgagtgcg tcaacctcaa acaaaacagc tcggcaaata
     1741 aacagatatt gccttgctgc gtgcttgcac cgccctctgg tcgcaatcag gatggttcta
     1801 tggaagcagt ggagactcga attcttcttt ttgatgatgt ggaagttttg gattttgcgg
     1861 gaccgtttga agtcttctcc atcgcggaga ctccggatca cagaaaagcg tttaacgtta
     1921 aaattgtagc ggaaagcaaa cgcactgtcg ttgccaggaa tggtctaaaa atcgtgccag
     1981 attcgagtga aacagggcgc tcgttttgtg gatcagggaa gaatcatcac gtccgccgga
     2041 atctcagccg gaattgatat gtctcttcat atagtagaaa gatactttgg aaaagaagct
     2101 tcacgcgcga cagcgcggag aatggaatac gattttataa gttgaaagcg ggcgttgagc
     2161 gccctcacac tccgcgcggc aagatcacag gctttccaga cgtcatattt tctgcgtatg
     2221 ccgcaattcc ttccggtgcc tgcgccagag tgagccgcgc ggaaaactcg gtatgaaaga
     2281 tttttctctc tacgagtttt tgaacctgcc gtgccgtttt tagaacccag agtgttccgc
     2341 gttccttgat gtgcgaagca agatgaaagc ccgtcacatt ctttctgaaa aacacaatgt
     2401 cttttgattt cgcagtcgca tcttctccgg acatagaacc atacactatg atttgagaac
     2461 gagaaggaag agcctgtaga acgcgtgcag acatggcacc gctgatgcaa tcgaatgcgg
     2521 tggttgcctt caggtcctgg gcgagtaccg gatcgtctcc ccagtcggga tggaggtcgc
     2581 gggcggtgcc ggggatcgcg gtgatattcg tcgccgggtt gctcgcctcg acctcggcct
     2641 cgccgacgac tccgctgatc gagtacgcct tcctcagcgc gtaggactcg gcgaccttca
     2701 ggatcatggc gctcgggtgc gagttccaga tgggcgtgtt gcgcttgtac tcgccgagcg
     2761 gcgcgtagaa gtaggtgatg ccgtgatcgc gccgctcgac catcgcccac gccccgatga
     2821 tgtggccgcg ctcggcgggg ttcttgaccg tgtggacgat gctccgctcg ccgttgatca
     2881 tgctggtttc gaacccgtcg ttcgaccaga ccacatcgcc gtccatcttc acgaagtcgg
     2941 gctggcggtg agcgtgtgcg aggaggccgt cgcg
//
LOCUS       DAORFF010000500         2959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_22531_length_2959_cov_3.564050, whole genome shotgun sequence.
ACCESSION   DAORFF010000500 DAORFF010000000
VERSION     DAORFF010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22531_length_2959_cov_3.564050"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKK76_16370"
     CDS             <1..456
                     /locus_tag="PKK76_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70107.1"
                     /translation="HNSKTKWPNLYVKKPEPGLEYAKILEILDKKNPRLVIALGDMCN
                     SFSDLKSSSGTDRAFSEPQPENYRKLFVKTRGRIYASGSKPGQFSFAMNSGGAFTNQW
                     IRALTTELKADNPAWKRLMTAATKPIKIGSAGQATQDPIYELVEESAPQ"
     gene            complement(506..2116)
                     /locus_tag="PKK76_16375"
     CDS             complement(506..2116)
                     /locus_tag="PKK76_16375"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNL70108.1"
                     /translation="MSRNHWWKTTTIYQVYPLSFQDSNGDGIGDLRGIIHRLDYIASL
                     GFETIWLSPFYISPFRDHGYDVSSYTEVDERMGTDADIQELIDRAHTWKLRVILDMVM
                     NHTSDQHPWFVESKSSLDSPKRNWYIWKKGKGNQPPNNWISMTGKPGWFRDRKTDEWC
                     YATFLEFQPDLNYRTPAVKTEMFEMAKGWLRRGADGFRLDIFNSIYKDDQFRDNPFSL
                     VYFPRPHALDECFFQRKKYNLNLPESVDFARDLRNATDSTGDKLLLGEVSGDDRAIRS
                     FLGNGEDALGLVFNFAAIEYQYNAHFFADILKRNEEHFSDPYLPTLVFGNHDVPRSIG
                     RVHGSIEKWKVLAMLQFSARGVPVSYYGEEIGMTNGDVAVADAQDPIAQANKLIPKFL
                     AHLLGVFLTRDDCRTPMQWDSSPNHGFSTAKPWNPIGKSPERTVAGQERDEHSLLTFY
                     RKLLAVRKSHAALHSGVAEEIKTAGSILSFDRVYGKEKASVYLNFSSISARVKGGAGT
                     VLIATGEVLQHKDEIELGPHSALILAHD"
     gene            complement(2186..>2959)
                     /locus_tag="PKK76_16380"
     CDS             complement(2186..>2959)
                     /locus_tag="PKK76_16380"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL70109.1"
                     /translation="IGGDTTCVEVDYRGTCIVMDMGTGARRAGLSIAKRALRDIYVLI
                     THTHWDHIQGFPFFAPAYDPGVTIHFYSCIPDLHERFIRQQHPHYHHVKFEDLPCKTT
                     FTYVEQGEQFEIGNLQMEAQPLIHPGGSASYKIRKENQTFVFATDTEFYGPDLSMLVK
                     KYDQFFHGADLLIIDAQYSLTEAEQKRGWGHTAMTIAVDCAMHWDIERLGLTHHEPAH
                     SDETIRELFARAHEYLEANNPHRHMMELTLAREGEEYFL"
BASE COUNT          697 a          716 c          769 g          777 t
ORIGIN      
        1 cacaattcga aaaccaaatg gcccaacttg tatgtaaaga agccagaacc gggtctcgag
       61 tatgcgaaga tcctggaaat cctggataag aaaaatccgc gtctggtcat cgcacttgga
      121 gatatgtgca atagtttctc agatctcaaa agcagtagtg gaacagatcg cgctttctct
      181 gagccccagc cagagaatta caggaagctc tttgtaaaga cgcgcggccg catctatgct
      241 tcaggatcga agcccggtca gttttctttc gccatgaata gcggtggagc ttttacgaat
      301 cagtggatca gagcactgac cacggagctc aaggcagaca atccagcctg gaagaggctg
      361 atgacagcgg ctacaaagcc catcaagatt ggaagtgctg gacaggcaac acaggatccg
      421 atctacgaac tggtagaaga gtccgctcca caataggcgg cttcacaggt tgttagatga
      481 gccccgtcgt ctggactcgc gtaattcaat cgtgtgcaag gatcaaagca gagtgtggcc
      541 cgagttcaat ctcatctttg tgttgcagca cttcccccgt tgcgatcaga acagttccgg
      601 caccaccttt cacgcgagcg gatattgagc tgaagttgag atagacgctg gccttttctt
      661 ttccatagac acgatcaaaa ctcaggatgg acccggctgt tttgatttct tccgccacac
      721 ctgaatgcaa tgcagcgtgc gatttgcgca ctgccaggag ttttctatag aacgtaagga
      781 gagaatgctc gtctctttcc tgaccggcca ccgttctttc cggtgacttt ccgattggat
      841 tccacggctt tgcggtcgag aaaccatggt taggtgaact atcccattgc atgggcgtgc
      901 gacaatcgtc tcgcgtgagg aatactccca gcaggtgtgc caggaatttt ggaataagtt
      961 tgtttgcttg agcgattgga tcttgcgcat ccgcaacggc cacgtccccg tttgtcatgc
     1021 caatctcctc tccgtagtag gagacgggca ctccacgtgc gctgaattgc agcatggcca
     1081 gaaccttcca cttttcaata gatccgtgaa cgcgtccaat ggagcgaggc acatcgtggt
     1141 ttccaaaaac aagagtgggg agatatggat cgctaaaatg ctcttcgttt cttttgagga
     1201 tgtccgcaaa gaagtgagcg ttgtattgat actcgatggc cgcgaaattg aaaaccagac
     1261 cgagtgcgtc ttctccattg cccagaaagc tgcgaatggc gcgatcatcg ccgcttactt
     1321 cacccagaag aagtttgtcc ccggttgaat ctgttgcgtt gcgcagatcg cgcgcgaagt
     1381 cgacgctttc gggaagattg aggttgtact tcttcctctg gaagaagcat tcatcgagcg
     1441 cgtgcggtct tggaaagtag acgaggctaa acggattgtc tctgaactga tcgtccttgt
     1501 aaatgctgtt gaatatgtcc aggcgaaatc cgtctgctcc tcgtctcagc catcctttgg
     1561 ccatttcgaa catttcagtt ttgacggcgg gggttctata gttcagatca ggctgaaatt
     1621 ctagaaaggt tgcgtagcac cattcatccg tctttcgatc gcggaaccat cctggcttgc
     1681 cagtcatcga aatccagttg ttgggaggtt gattcccttt tcccttcttc cagatatacc
     1741 agtttctttt tggagaatcc agtgagcttt tggattctac aaaccatgga tgctggtccg
     1801 aagtgtggtt catgaccatg tcgaggataa cgcgcagctt ccacgtatgg gctctatcaa
     1861 tgagctcttg aatgtccgca tctgttccca ttctttcgtc tacttcggtg taggagctca
     1921 cgtcatagcc atgatcgcga aacgggctta tgtaaaacgg agacagccag atcgtttcaa
     1981 agcccaggga agcaatgtag tcgagccggt ggatgatgcc gcgaagatcg ccgattccat
     2041 ctccattgga atcttgaaac gaaagaggat agacctgata gatggtcgtg gttttccacc
     2101 agtggttgcg gctcatgtcc gagtctggtc actggcagcg cgcaattcaa tcaattattg
     2161 caaatcaggc gctgtcccgg gcagtttata ggaaatactc ttcgccttct cgcgccagag
     2221 tgagttccat catgtgcctg tgcggattgt ttgcttccag atattcatgg gccctggcga
     2281 acagctctcg aatggtttca tctgagtgcg ccggttcatg atgggtgagg cccagtcgtt
     2341 ctatatccca gtgcatggcg cagtccactg cgatggtcat ggctgtgtgg ccccatcctc
     2401 ttttttgttc tgcttccgtc agggaatact gcgcgtcaat gatcagcaag tcggcaccgt
     2461 gaaagaactg atcgtatttt ttgacgagca tggaaagatc tggaccgtag aattcggtgt
     2521 ccgttgcaaa aacgaacgtt tgattttcct tacgtatctt ataagatgca gagccgcctg
     2581 gatgaatcaa tggctgtgct tccatttgga gattgccaat ctcaaactgc tctccctgtt
     2641 ctacgtaggt gaatgtagtc ttgcatggca gatcttcgaa tttaacgtga tgatagtgcg
     2701 gatgctgctg ccgaatgaat ctttcatgaa gatctggaat gcaagaatag aagtggatgg
     2761 tgactccagg atcgtaagca ggtgcgaaaa atggaaagcc ctggatatga tcccagtgtg
     2821 tgtgagtaat gagaacgtag atgtcgcgta acgcccgttt ggcgatggat aagcctgccc
     2881 gtcttgcgcc ggttcccata tccataacaa tgcacgtgcc gcgatagtct acttccacgc
     2941 aggttgtatc tccgcctat
//
LOCUS       DAORFF010000501         2959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_22534_length_2959_cov_3.438361, whole genome shotgun sequence.
ACCESSION   DAORFF010000501 DAORFF010000000
VERSION     DAORFF010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22534_length_2959_cov_3.438361"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1795
                     /locus_tag="PKK76_16385"
     CDS             <1..1795
                     /locus_tag="PKK76_16385"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL70110.1"
                     /translation="SRTPRDLYLIIRAPLLGRVTLFEGERVRELGAEKPFSSREIFHR
                     YFIFPIHIEKQTTLYLRVQTKGLVGIPVRIMTPAAFAQSDWIETLLLGIYYGTMLCML
                     VYNLFVLVIIRERSYVSYLLYVSAGTVYALSTNGLAFEFLWPNTPALTYRFNGPWVCL
                     YIALSLQFTRSFLQTVTTAPWLNRWIEFSGIIALASSLSFLLVEPQTTGKIAAAMATP
                     CLLTMLIAGIRVMMSGFRPARLFTLAVVVYVIGGIVFTFRNWGYLPANNMTLYGIQFG
                     GMIQAVVFSLALADRVQVLRSNLKKHVEELETMKAAIELSEQKYRKLVEDTQDIIFSL
                     DRHFAFLTVNRAIGSHLGYSPKKIIGQRLQDITYTARSWKGELLTFQLKELETRDHTE
                     FRQAFKTSLGESRDLQVRLELLRDDGTILGKAQSPADDPLQAFFYSERTRYYVDNLIS
                     TSELASHRISRNLRRYCSEEDSQQIKMAVREMVLNAVEHGNLEIDFDTKTRAMAQGAY
                     FDLLLARQRDPAYAGRKVKVDVSLNPNRVIFQVEDEGRGFDHESAKARADQANEERLE
                     HGRGIKMTLAIFDLVQYNTRGNRVTLVKFFH"
     gene            complement(1789..2415)
                     /locus_tag="PKK76_16390"
     CDS             complement(1789..2415)
                     /locus_tag="PKK76_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70111.1"
                     /translation="MDPVFERTCDLIDGELENWLQAVLGDILTSYDRLHLRDPLCTCL
                     LELATNANKANMKHIFFLEKGWDIYDPQLYTERLKEFRDHVLHFESTVAYAEKAKQAG
                     LYVRVSIHHSPAGLKIAVYNNLPVIPEDEKRIREKFLHALEYQNLVDFYRDYGDNSEG
                     SGLGFALMVLMLRSEGIPPSLLRVGTDGGQTAARIEVPFHPGSAPQSQ"
     gene            2517..>2959
                     /locus_tag="PKK76_16395"
     CDS             2517..>2959
                     /locus_tag="PKK76_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70112.1"
                     /translation="MLRCAAAVCAMLFFLDCEPGKSLRIGTNIWPGYAPLYLARDRKG
                     WEPGNIRLVEYPSATEVLRAFQGGALECASLTLDEALMVRESGIPFSVILIHDISQGA
                     DAIVARPLIADVKGLRGKRIGVEASALGAFVLTRALEKNKMTSAD"
BASE COUNT          747 a          788 c          713 g          711 t
ORIGIN      
        1 gtcacgcacc cctcgtgacc tgtacctgat cattcgcgcc cctcttcttg ggcgggtaac
       61 gctgttcgaa ggcgaacgcg tacgcgaact cggcgcggag aagccattct cttcgcgcga
      121 aatctttcac agatacttta tctttcccat tcatatagaa aaacaaacca ctctttacct
      181 gcgcgtgcaa acaaaagggc tcgtgggaat tccagttcgc atcatgactc ccgccgcatt
      241 cgcgcaatct gactggatcg aaactttgct actcgggatc tactacggca ccatgctttg
      301 catgctggtc tacaacctct ttgttctggt gatcatacgc gaaagaagct atgtcagcta
      361 tttgctttat gtttccgcag gcaccgtata cgccctctcc acaaacggac tcgcgtttga
      421 atttctctgg ccaaacacgc ctgccctcac atatcgcttc aacggaccct gggtttgcct
      481 ctatatcgct ctatcccttc aattcacccg aagctttctt caaacagtca cgactgcacc
      541 ctggctcaat cgctggattg aattctctgg aatcatagca cttgcttcga gtctatcttt
      601 ccttctggta gaacctcaga caacgggaaa aatcgcggcg gccatggcca ccccatgcct
      661 cctcaccatg ctcatcgccg gaattcgagt gatgatgtct ggctttcgcc cggctcgact
      721 cttcactctg gctgttgtgg tctatgtgat aggaggaatt gtattcacgt ttcgcaactg
      781 gggatatctt cctgccaata atatgacgtt atacggcatt caatttggag gaatgatcca
      841 ggccgtcgta ttttcccttg ctctcgctga ccgagtgcag gtactgaggt cgaatcttaa
      901 gaagcacgtt gaagaactcg aaacaatgaa agcagccatc gagctttccg agcagaaata
      961 cagaaaactg gtggaagata cccaggacat cattttctct ctcgatcgcc actttgcatt
     1021 cctgacagtc aatcgcgcca tcggatctca tctgggctac tctccaaaga aaatcattgg
     1081 gcaaagattg caggacatca cttacaccgc gcgttcctgg aaaggcgagc tacttacttt
     1141 tcagctaaag gagctggaaa ccagagacca tactgaattc aggcaggcat tcaaaactag
     1201 cctcggagaa tcgcgagact tgcaggtgcg tctcgaactt ttgagggatg acggaaccat
     1261 tcttggcaag gcgcagagtc ccgcagacga tccgctgcaa gctttcttct attcagaaag
     1321 aaccaggtac tatgtggata acctcatcag cacctcagag ctcgcttcgc acagaatatc
     1381 gcgcaacctg cgacgatact gcagcgaaga agattcacag caaatcaaga tggctgtcag
     1441 agaaatggta ctcaatgcag tcgagcacgg aaacctggaa atcgacttcg atacgaagac
     1501 gcgcgctatg gcgcagggtg cttacttcga tttgcttctg gcccgccaaa gggatcctgc
     1561 ttatgcgggt agaaaagtga aagtggatgt ttcactcaat ccaaatcgag tcatattcca
     1621 ggtggaggac gaaggccgcg gatttgatca cgaatccgca aaagcaagag cagatcaggc
     1681 aaatgaagaa aggctagaac atggacgcgg catcaagatg actctggcga tatttgatct
     1741 ggtgcaatac aatacgcgcg gcaatcgcgt aacactggtt aagttctttc actgagactg
     1801 aggagcgctc cccggatgga agggtacttc gattctggca gcagtctgac cgccatccgt
     1861 tccgaccctc aaaagagacg gcgggatgcc ttcgcttcga agcatcaaaa ccatcagcgc
     1921 aaatccaaga cctgagccct cgctattgtc tccgtaatca cgatagaagt cgaccagatt
     1981 ctgatactcc agagcgtgca aaaacttttc acgaatcctt ttttcgtctt ccggtatgac
     2041 cggtaagttg ttgtacactg caatcttcaa accggcaggc gaatgatgaa tggaaacacg
     2101 aacgtacagg ccagcttgct tcgctttttc cgcgtatgcg accgtacttt caaagtgcag
     2161 gacatgatcg cgaaactctt tcagtctctc tgtatacaat tgcggatcat aaatatccca
     2221 gcccttctcc aggaaaaaaa tgtgcttcat gttcgctttg tttgcgttgg tcgcaagctc
     2281 aaggagacag gtacaaagag gatcacgcaa atgaaggcga tcatacgagg ttagaatatc
     2341 ccccagcact gcctgaagcc agttctccag ctcaccatct ataaggtcgc acgttctctc
     2401 gaagaccggg tccattcgtt tcacaatatt accccccgca gcagcggcag gcaatacttt
     2461 aaggccgatc ggtcaaaaag ttgatgtggt ttcccagggg aaaaacatac tgtcttatgc
     2521 tccggtgcgc cgccgcagtc tgtgccatgt tgttctttct cgactgcgag ccgggtaagt
     2581 ccctgcggat cggaacaaat atttggccag gatacgctcc tctctatctg gcgcgggacc
     2641 ggaagggatg ggaaccagga aacattcgcc tggtcgaata tccttcggct acggaagtgc
     2701 tgcgcgcatt ccagggcggc gctctcgagt gcgcttccct gaccctggat gaggctttga
     2761 tggtgcgcga aagtggcatt ccattttccg tcatactgat tcatgacatt tcccagggag
     2821 cagacgcaat tgtagcccga ccactcattg cggatgtaaa agggctgcgt ggaaaaagaa
     2881 tcggagtgga agcatccgcg cttggcgcat tcgttctcac acgggctctt gaaaagaata
     2941 aaatgacgag cgcggacat
//
LOCUS       DAORFF010000502         2955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_22599_length_2955_cov_2.779310, whole genome shotgun sequence.
ACCESSION   DAORFF010000502 DAORFF010000000
VERSION     DAORFF010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22599_length_2955_cov_2.779310"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PKK76_16400"
     CDS             <1..406
                     /locus_tag="PKK76_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HNL70113.1"
                     /translation="WDPQENYYYCREHTGFTPNPERSQGTYSKYASLDDKIDGFHYYL
                     AYIKFGIGRATSDAAHEIRDGKITREEGVALVKRYDGEFPHKYFDEFLDYCNITEAEV
                     HEVIDSWRSPHLWEQKDGVWQLKYASYLDTPV"
     gene            431..1198
                     /locus_tag="PKK76_16405"
     CDS             431..1198
                     /locus_tag="PKK76_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HNL70114.1"
                     /translation="MSTIRLIGRLDIKGPNLIKGIHLEGLRVIGDPQEFARKYYEQGI
                     DELVYMDIVASLYGRNSLHDIIRSAAHDVFVPLTVGGGIRSVDDAREILRAGADKVAI
                     NTAAVKRPDLIREIAHVFGSQCMVLSIEAKCQAKDRWEVYTDNGRERTGLDVVEWAKR
                     AVGLGAGEILLTSVDQEGTRKGFDCDLVRAVSDSVSVPVIASGGMGTVEHLKQVVTDG
                     HADAVAMADILHYNRSTLAEIRSSAAALGVSVRSDEL"
     gene            1188..1835
                     /gene="hisH"
                     /locus_tag="PKK76_16410"
     CDS             1188..1835
                     /gene="hisH"
                     /locus_tag="PKK76_16410"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNL70115.1"
                     /translation="MSFDVVVIDYGIGNLYSVRRAFEACGANVVLTDDPALIVSAPRL
                     VLPGVGAFADGMRGLIERGLDSVVREYAKSGKPLVGICLGMQMLATVSMEFGEHRGLN
                     LIPGKVVRIPSTTMEGAPHKIPHIAWEALVRPDFIETWDNTILANTRPGATMYHVHSF
                     TVVPDDDAHRLADCYYNGRQISSVIRMGNVYGCQFHPEKSGRAGLALLKRFLEMS"
     gene            1870..2874
                     /gene="pseB"
                     /locus_tag="PKK76_16415"
     CDS             1870..2874
                     /gene="pseB"
                     /locus_tag="PKK76_16415"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HNL70116.1"
                     /translation="MLNQKSILVTGGTGSFGKRFVGTILKQFPDIKRLVVYSRDELKQ
                     FEMAQEFPESQYKQIRYFIGDVRDKERLSYAVEGIDTVVHAAALKQVPAAEYNPLEAI
                     KTNILGAQNLIEACIDKGVKQVVALSTDKAAAPINLYGATKLCSDKLFIAANLYRGNH
                     DIKFSVVRYGNVMGSRGSVIPFFLKQKEKGFLPITHPGMTRFNITLDEGVNMVLFALA
                     NMWGGEIFVPRIPSYRILDVAEAIAPGMEKRIVGIRPGEKIHEEMITETDAISTIEFE
                     KHFVILPSFQPAWNIDEFRSHFKGSFCKEGFRYNSGENSDYLSVEQLRNLIQSAAIKY
                     "
BASE COUNT          753 a          692 c          773 g          737 t
ORIGIN      
        1 ctgggatccg caggagaact attactactg ccgtgagcat acaggcttca ctccgaatcc
       61 agaacgttct cagggcacat actcaaagta cgcgagtctc gatgacaaga tagacggttt
      121 tcactattac ctggcgtata tcaaattcgg aattggccgc gcgacatcgg atgcggcgca
      181 tgagatccgt gacggcaaaa ttacacgcga agagggtgta gcgctggtta agcgatacga
      241 cggtgaattt cctcacaaat attttgacga gtttttggac tactgcaaca ttaccgaagc
      301 agaggttcat gaagtgatcg acagctggcg atcgccccac ctgtgggaac agaaagatgg
      361 tgtgtggcag ctcaaatatg cttcttatct tgatacgccg gtctaagtca cgcccgtaca
      421 aaattcatcc atgagcacca ttcgactcat cggccgtctc gacataaagg gaccgaacct
      481 gatcaaaggc attcatctcg aaggccttcg agtcatcggg gaccctcagg agtttgcccg
      541 caagtattac gaacagggca tcgatgaact cgtttacatg gatattgttg caagtctcta
      601 cggtagaaac agtctgcacg atatcattcg cagcgctgcg cacgacgttt ttgttcctct
      661 cactgtggga ggtggaattc gttctgtaga tgatgcgcga gaaattttgc gcgcaggtgc
      721 agacaaagtc gccatcaata ctgccgcggt gaagcgtcct gatttgattc gggaaattgc
      781 ccacgtattt ggatcccagt gcatggttct ttctattgaa gcgaagtgcc aggcaaaaga
      841 tcgctgggaa gtttacacag acaatggccg cgagcgaaca ggcctggatg tggtggaatg
      901 ggcaaagcgt gctgtcggtc tgggtgcagg cgaaatcctt ttgacttctg tcgatcaaga
      961 aggaacgcga aagggctttg attgcgatct cgttcgcgca gtgagtgatt cggtttctgt
     1021 cccggtcatt gcaagcggtg ggatgggtac agtcgaacac ctcaagcagg tggttacaga
     1081 tgggcacgcg gacgcggttg cgatggctga tattttacac tacaatcgtt ctactctcgc
     1141 ggagattcgc tcatctgcgg ccgcactcgg agtttcggtg agatcggatg agctttgacg
     1201 tagtagtaat cgattacggg atcgggaatc tctacagcgt tcggcgcgct tttgaggcat
     1261 gcggagcaaa tgtagttctt acagatgatc ccgcactcat cgtatccgct cccagactgg
     1321 tactgcctgg agtgggggct ttcgcagacg gcatgcgagg cctcattgag cgaggacttg
     1381 attctgttgt tcgcgaatat gcgaaatcag gaaagcctct cgtgggaatt tgtctgggaa
     1441 tgcagatgct tgcaaccgtc agcatggaat ttggagaaca tcgcggactc aatctgattc
     1501 caggcaaagt ggttcgaatt ccttctacca ccatggaagg agcgcctcac aagattcctc
     1561 acatagcctg ggaagctctg gtgcgccctg atttcattga aacgtgggac aacacaattc
     1621 ttgcgaatac cagaccaggt gcaaccatgt atcacgtgca ttcattcact gtggttccgg
     1681 atgatgacgc gcacagactc gcagattgct actacaacgg gcgccagatt tcttctgtga
     1741 tccgcatggg caatgtgtat ggctgccagt ttcatcctga aaagagcggc cgtgcaggcc
     1801 ttgcactttt gaagcgtttt cttgaaatgt cctagtggtt aagaaaattt attaaaaaga
     1861 agtaagattg tgctgaatca gaaatccatt cttgtaaccg ggggcactgg ctcctttgga
     1921 aagcgattcg tgggaactat cctcaagcag ttccccgata tcaagcgtct cgtggtctat
     1981 tcgcgtgatg aactgaaaca gtttgagatg gcgcaggaat ttcctgaatc gcagtacaag
     2041 cagattcgct atttcattgg tgatgttcgt gacaaagagc gtctttcgta tgcggtagaa
     2101 ggaattgaca cggtggtgca cgcggcggcc ctcaagcagg ttcccgcggc agaatacaat
     2161 cctctcgaag caatcaagac aaacatcctg ggcgcgcaga atctgattga agcatgcatt
     2221 gacaaaggcg ttaaacaggt tgttgcattg tccacagaca aagctgccgc gcccatcaac
     2281 ctttacggag caaccaaact ctgttcagac aagcttttca tcgcagcaaa tctttatcgc
     2341 ggaaatcacg atatcaagtt ctctgttgta cgctatggaa atgtgatggg aagccggggc
     2401 tctgtgattc cgttctttct caaacaaaaa gaaaaggggt tcttgcccat cactcatcca
     2461 ggcatgactc gcttcaacat cactctggat gaaggcgtga atatggtgct gttcgctctt
     2521 gcaaacatgt ggggcggaga gattttcgtg ccgcgcattc ccagctatcg cattctcgat
     2581 gttgcagagg caatcgctcc aggcatggaa aagcgaatcg tgggcattcg tcccggtgaa
     2641 aagattcacg aggaaatgat tacagaaacg gatgcgatca gcaccatcga attcgaaaaa
     2701 cactttgtca ttctaccttc ttttcaacct gcctggaaca tcgatgaatt tcgcagccat
     2761 ttcaaaggta gtttttgcaa agaaggattt cgctacaaca gcggcgagaa ttcggattat
     2821 ctatcagtcg agcaattgcg aaatctaatt cagagcgctg ccattaaata ctgatgactt
     2881 ctattcgtcg cattccctac ggcagacaaa ccataacgca ggaagatatc aatgcagtgg
     2941 ttgaaacgct gcgtt
//
LOCUS       DAORFF010000503         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_22665_length_2950_cov_3.529188, whole genome shotgun sequence.
ACCESSION   DAORFF010000503 DAORFF010000000
VERSION     DAORFF010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22665_length_2950_cov_3.529188"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..91
                     /locus_tag="PKK76_16420"
     tRNA            19..91
                     /locus_tag="PKK76_16420"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52..54,aa:Val,seq:tac)
     gene            153..1997
                     /locus_tag="PKK76_16425"
     CDS             153..1997
                     /locus_tag="PKK76_16425"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL70117.1"
                     /translation="MSFAPMLRSLLNRITNLVPEDDRGIRWTIRLKLSLIISLLVGLA
                     LFLIISLASLFFRRDNEVRIQESSRQLAANVGTAVSAELKSRIQNLRLLSESLGAARG
                     EKVLFENDRSLALVGIFQVQGDSIAPRTVLVNAESFKDSRGDIQQALVRLYPAFARAR
                     NGSIVVRNVSPDLRVPLCAIAFPSRIQGGDRIIVMVLRLDELLAMFQTGGIIKSALVN
                     EEGTVIAHEDRDTVVSGTSFASVPIVKEMMASKLNNGLTEYDFKGVSYWGAFRRLDFG
                     NVGVITYAEGSKWFEAVDRILKWNFLLMIVVICVAIIFVFFFARSFSEPILKLVGAAS
                     DIENGRFILNLKPETSDEIGLLTKSFASMGRGLQERENLKVSFGRFVNKELAEMAMSG
                     ELDLGGVSKEVTIFFSDIRGFTTISEKLEPEEVVEFLNQYMTEMVKCVQQTYGLVDKF
                     IGDAVMALWGALRPHGNDAVNAVNCAILMRKQLMKFNRGRGSARKPIIRIGMGINTGH
                     VIAGQMGSAEKMDFTVIGDAVNTASRVEHLNKTYGTDILITDHTYEKTGKLFKVEEVD
                     RVAVRGKSKPVTLYAVLGLADDKDTPSNIKELRKMVGIEFNAAAAKKG"
     gene            2003..>2950
                     /locus_tag="PKK76_16430"
     CDS             2003..>2950
                     /locus_tag="PKK76_16430"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNL70118.1"
                     /translation="MDRLREIRIPLIVGSVAVMALLLFICDYTRRGDIGSREEIGSII
                     FRRHGVQRKFVDEVVWERIEQGTRLANSDSIRTDDKSEVKIRLKDGTVLTVDQNSMIQ
                     LDVASNRIRFETGSMQVQSSGSSSLTLQSGDSKVQMGEGSLTLSKSEKQDLSLTVYEG
                     TAKVTSGGKTENVGKNQKADIGKEGISVKEVPVVLIKPNPFQNVPAEGDGTSVSFKFQ
                     PSDSLKKPTLEISKDQEFKDSRKVQTPGVATMDLPPGTYYWRVTGEDAKTNQPAMSET
                     RKMTIVRGEPLRLHSPGSGERVAFNSNPPPVPFAWSQDPL"
BASE COUNT          771 a          703 c          767 g          709 t
ORIGIN      
        1 cccacgggtc accaatatgg acgattagct cagctgggag agcgcctgcc ttacaagcag
       61 gatgtcggca gttcgatcct gtcatcgccc acatctctac tcccgtttaa aacatcttcc
      121 tccaataaaa gattggcgtt tcgcgcttcc gtgtgagctt tgctccaatg ctgagaagcc
      181 ttctcaatcg aatcacaaac cttgtcccgg aggatgaccg cggcattcgc tggaccatcc
      241 gactcaagct gagcttgatc atttcccttc ttgtgggtct tgctcttttt ctgattatct
      301 cgctggcatc gcttttcttt cgccgcgaca acgaagttcg tatccaggaa tcaagccgtc
      361 agctggcggc caacgtgggc actgcggttt cagcagaatt aaaatccaga attcagaatc
      421 tgcgtctcct ttctgaaagc cttggtgctg cgcgcggaga gaaagttctc ttcgaaaacg
      481 atcggagtct tgctcttgtt ggaatcttcc aggtgcaggg cgattctatt gcgcccagaa
      541 ccgtgcttgt aaatgcagaa tcgtttaagg actccagagg agatatccag caggccctcg
      601 taagattgta tccggctttt gctcgagctc gcaatggatc catcgtagtg cgaaatgtga
      661 gtcctgatct ccgagtcccg ttatgcgcca ttgcttttcc atctcgcatt cagggtggag
      721 acagaatcat tgtgatggta ttgcgtctcg atgagttgct tgcgatgttt caaacgggtg
      781 gcattatcaa gtccgctctt gtcaacgaag aaggaactgt catcgcgcac gaagacagag
      841 acacagtggt gtccggaacc tcttttgcgt ctgtgcccat cgtaaaagaa atgatggcaa
      901 gtaaactcaa caatggcctc acagagtacg atttcaaagg cgttagttac tggggagcat
      961 tccgcagact cgactttgga aacgttggag tcattactta cgcagaaggc agcaagtggt
     1021 ttgaagctgt ggatcgaatc ctcaaatgga atttccttct catgatcgta gtcatttgcg
     1081 tcgcgatcat ttttgttttc tttttcgcgc gcagcttctc tgaaccaatc ttgaagcttg
     1141 tgggtgctgc tagcgatatt gaaaacggtc gcttcattct gaatctgaaa ccagaaactt
     1201 cagacgaaat tggactgttg acaaagtcgt ttgcatccat gggtcgcggc cttcaggaac
     1261 gcgagaatct caaagtatct tttggaaggt ttgtgaacaa ggaactcgca gaaatggcca
     1321 tgtccgggga actggacctt ggtggagtga gcaaagaagt aaccatattt ttttcggata
     1381 tccgcggttt cacgacgatt tctgagaaac tagaaccgga agaagtcgtc gaatttctga
     1441 atcagtacat gacagaaatg gtgaagtgcg tacagcaaac atacgggctc gtggataagt
     1501 tcattggaga tgctgtgatg gctttgtggg gcgctcttcg tcctcacgga aacgacgcag
     1561 tcaatgcagt caattgcgcc atcctcatgc gcaagcaact catgaaattc aatcgcggtc
     1621 gagggtctgc cagaaagccg atcattcgca ttggaatggg aatcaataca ggccatgtga
     1681 ttgctggaca gatgggttcg gcggagaaaa tggacttcac ggtcattgga gacgcagtga
     1741 acaccgcaag ccgtgtggaa catctgaata agacctacgg cacagacata ctcatcacag
     1801 atcatacgta tgaaaagacc gggaaactct tcaaagtgga ggaagtggat cgggtcgcag
     1861 tacgcggaaa aagcaaacct gtaactcttt atgcggttct ggggctggcg gatgacaaag
     1921 ataccccgtc taatatcaaa gaactgcgca agatggttgg cattgaattc aatgcggccg
     1981 ctgcgaagaa aggctgaggt cgatggacag attacgcgaa attagaatcc cgctcattgt
     2041 gggctctgtg gcagtcatgg cgctgcttct ctttatctgc gactacactc gtcgcggtga
     2101 cattggatcg cgcgaagaaa tcggatctat catatttcgc agacacggcg tgcagcgcaa
     2161 attcgtggat gaagtagtct gggaacgaat cgagcaggga accaggcttg ccaattcgga
     2221 cagcattcgc acggacgaca aatctgaagt caaaattcgc ctgaaagacg gaactgtgct
     2281 tactgtagat cagaattcaa tgatccagct ggatgttgca tccaatcgca tacgattcga
     2341 aacaggatcc atgcaggttc aatcgagcgg ctcatccagt ctcactctgc agtcaggaga
     2401 tagcaaggtg cagatgggcg aaggtagcct gactctgtcg aaatcagaaa aacaggatct
     2461 cagtctcaca gtctacgaag gcacagcaaa agtaacgtca ggcggcaaga cggagaacgt
     2521 aggcaagaat cagaaagcag acattggcaa ggaaggaatc tccgtcaaag aagtgccagt
     2581 cgttttgatc aaacccaatc catttcagaa tgtaccggca gaaggggacg gaacatctgt
     2641 atctttcaag tttcagcctt ccgattctct aaagaagcct actcttgaaa ttagcaagga
     2701 ccaggaattc aaagactcac gaaaggtgca gaccccagga gtcgccacta tggatcttcc
     2761 gccggggaca tactactggc gcgtgacagg cgaagacgcg aaaactaacc agcccgccat
     2821 gagtgaaacg cgcaagatga cgattgtgcg cggagagccg ttgcgccttc attctcccgg
     2881 ttccggggaa cgcgtcgcat tcaattcgaa tcctccgcct gtgccgttcg cctggtctca
     2941 ggatcctctt
//
LOCUS       DAORFF010000504         2924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_23017_length_2924_cov_3.291042, whole genome shotgun sequence.
ACCESSION   DAORFF010000504 DAORFF010000000
VERSION     DAORFF010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23017_length_2924_cov_3.291042"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PKK76_16435"
     CDS             complement(<1..1024)
                     /locus_tag="PKK76_16435"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNL70119.1"
                     /translation="MIRILFQLLTLPLRFLWLLYCRIRNVRKPGSILVHRIPDRFTVF
                     RSGGVLSMFASRDDVHFAEYLALLGLMEEEREIQTVLISIPDVQHNMAETEQIAAALD
                     RIVKSGKKVIGHSEGGGIRTLYLLSCCSERFASPASSYSSFFPASEPHYFKGLLKKFG
                     VRMEVFSAGKYKSAGETFSRSGPSKEARENLEELISGLRANIARGMEARAPGFAKKFK
                     NKLLWSASELVTEGFFSREITEKDLARHLSGEPRETVKHAFSTVQPELEQKGPQLRIT
                     SDGALVRRHLRSRFKLLNLSRRRSFAFVAMEGAIYSGRKGDHARSGMISGHAYRDLLH
                     DLADSRD"
     gene            1088..1579
                     /gene="ilvN"
                     /locus_tag="PKK76_16440"
     CDS             1088..1579
                     /gene="ilvN"
                     /locus_tag="PKK76_16440"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016560825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNL70120.1"
                     /translation="MKHILSILVKNRPGVMSHVSGLFTRRSYNIDSIAVGVTENPAVS
                     VITIVLKGDEKALGQFQGQLLKLPDVVEVKSLPFHDSLIRELVLVRIKSDGSKRNEIF
                     GIVEVFGGKVAEVTEDSILIEIHGSGRQIHAAIDILAQFGIIEMARTGAIALAYRTDT
                     GAG"
     gene            complement(1581..2768)
                     /locus_tag="PKK76_16445"
     CDS             complement(1581..2768)
                     /locus_tag="PKK76_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70121.1"
                     /translation="MRFYFRISVAALAVSLLLCKGGNSPASARENSESHREWNDIARF
                     LAGMEPDAGSTISDLEGLPAAESQRAYFAKIWPEVEKTQLEPQRKWRDEVLGKTEGFI
                     FYPFSGADFLNIYTFFPQGSEYVFFGLEPPGPPPSVKGMKPADVNQSLAGLQVSLRSI
                     LNYSFFQTLHMVNDLKATKLSGVAPVLLVFAARTGNKVHSVHNIRLHPDGKIESLPDP
                     FSGKPEPAATSEDYVSGIRVVFQKPGATEYTHMDYFSIDISEEGLAKKPYFLKYIQSL
                     GTPTTYLKAASYLMYNKDFTTIRNFILDESKLILQDDSGIPLQYFKPQSFDLTFYGSY
                     TRPIPLFDGKYQKDLYEMYLPKNHPKPLPFGIGYKFRKGDSNLMLAKRKTDRPDIPAP
                     SKQ"
BASE COUNT          768 a          717 c          726 g          713 t
ORIGIN      
        1 cgtctcttga atctgccaga tcatggagca aatcgcgata cgcatgcccg gaaatcattc
       61 ctgatctggc atgatcccct tttcgacccg aatagatagc tccttccata gcaacaaaag
      121 caaagctccg gcgtcgactc aggttcaaaa gcttgaacct ggatcgcaaa tgacgacgaa
      181 ccagagctcc atcggacgtt atgcgcaatt gaggtccttt ttgttcgagt tctggctgca
      241 cggttgagaa agcatgcttc actgtttccc tgggctcccc cgaaaggtga cgcgccagat
      301 ccttttccgt gatctcgcga gaaaagaatc cttccgtcac aagctcagaa gcagaccaga
      361 gaagcttgtt tttgaatttc ttcgcaaagc ccggggctct cgcttccatt cctctggcaa
      421 tgttcgcacg cagcccgctg atgagttctt ctagattctc gcgcgcttct ttcgagggtc
      481 cggaacgact gaatgtttct ccggcgctct tgtatttgcc cgctgaaaaa acttccatgc
      541 gaacaccaaa tttcttgagc aaccctttga agtagtgagg ttcgcttgcg gggaagaaag
      601 atgaataaga agaagcaggc gaggcgaatc gctcactgca gcaggagagc aaataaagcg
      661 tgcgaattcc acctccttcg ctgtgaccga ttactttctt tcctgatttt acaatgcgat
      721 caagtgccgc cgcaatctgt tccgtttccg ccatattgtg ctgaacgtct ggaatggaaa
      781 tcagaacagt ctggatttcg cgctcttctt ccataagacc gagcaatgcc agatactccg
      841 cgaaatgcac atcatcgcgt gatgcgaaca tagacagaac gccaccgctg cgaaatactg
      901 tgaatctgtc cgggatcctg tgaaccagaa tcgaacctgg tttgcgaaca ttacgaattc
      961 tacaatacag caaccagagg aatcgcagag gtagagttag aagctggaag agaatccgaa
     1021 tcatgctgca ccgccatttg cgcttgacgt agaagcttcc cgtccgatgg ggagatggga
     1081 gtccaacttg aagcacatat tgtccatcct tgtaaaaaac cggcccggtg tgatgagcca
     1141 tgtatcaggc ctcttcacgc gccggagcta caacattgac agcatcgccg ttggagtcac
     1201 ggaaaatcca gccgtctctg taatcacgat cgtgctaaaa ggagacgaaa aggctctggg
     1261 tcaattccag ggacaacttc tcaagttgcc cgatgtagta gaggtgaaaa gccttccatt
     1321 tcacgactct ctgatccggg aactggttct ggtgcgaatc aagtcagacg gctcaaaaag
     1381 aaatgagatt tttggaattg tagaagtgtt tggcggaaag gtcgcggaag taacggaaga
     1441 ttctatcctc attgaaatac acggaagcgg cagacaaatt cacgccgcca tagacatcct
     1501 ggcccagttt ggtattatag agatggcgcg caccggcgcc attgcccttg cgtatcgcac
     1561 ggacactggc gcgggttaac tcactgcttt gacggagcgg gaatgtcagg acgatctgtc
     1621 ttccgctttg ccagcatcag gttagaatca cctttgcgaa acttatagcc aatcccgaac
     1681 ggaagtggtt taggatgatt cttagggaga tacatttcgt atagatcttt ctgatatttc
     1741 ccatcaaaca atggaatggg tctcgtgtaa gacccgtaaa acgtaagatc aaaactctgt
     1801 ggcttgaagt actgaagagg aattcctgaa tcatcttgaa ggatgagctt gctttcgtcc
     1861 aggatgaaat tgcgaatagt tgtaaaatct ttattataca tcaaataaga agcagccttg
     1921 agatacgtgg ttggcgtacc cagactctga atgtatttca ggaagtaagg cttctttgcc
     1981 agaccctctt ctgaaatatc aatggagaaa taatccatgt gagtgtattc agtggctcct
     2041 ggtttttgaa acacaacgcg aattcctgaa acgtagtcct ctgaggttgc agccggttca
     2101 ggttttccgg aaaacggatc cggcagagat tcaatcttcc catcaggatg caggcgaatg
     2161 ttgtgcactg aatgcacttt gtttcctgtt cgcgctgcaa atacgagaag cacgggtgcc
     2221 acaccggaaa gcttggttgc tttcaagtcg ttgaccatat gcagagtctg aaagaacgaa
     2281 tagtttagaa tgctgcgaag cgaaacctgg agaccggcca gactctgatt tacatccgcc
     2341 ggtttcattc ctttcacaga aggcggaggt cctggaggtt cgagaccaaa gaagacgtac
     2401 tctgaccctt gcggaaagaa agtatatatg ttcaggaaat cagcgccgct gaaagggtag
     2461 aagataaacc cttccgtttt tccaagcacc tcatcgcgcc acttacgctg cggctcaagt
     2521 tgagtttttt ccacttctgg ccagatcttt gcgaagtacg cgcgctggct ttcggctgct
     2581 ggaaggcctt ctagatcaga gatagtgctg cctgcatccg gctccatgcc tgcgaggaag
     2641 cgcgcaatgt cgttccattc gcgatgcgac tctgaattct cacgggctga tgccggcgaa
     2701 ttgccgcctt tgcagagcaa caatgaaacg gcgagtgccg cgacagaaat acgaaaataa
     2761 aaacgcatga gtttatagaa aaaaactcga cctcaagtca attataattt gccgcgaagg
     2821 tcagggcgcc gatgcttgta aagcgcgcgc actttttgcg ccaccgtctt tcccaaatga
     2881 acaacacgga agctctagca cgccggcttg gattctggag cgca
//
LOCUS       DAORFF010000505         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_23066_length_2920_cov_3.218848, whole genome shotgun sequence.
ACCESSION   DAORFF010000505 DAORFF010000000
VERSION     DAORFF010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23066_length_2920_cov_3.218848"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="PKK76_16450"
     CDS             <1..1313
                     /locus_tag="PKK76_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70122.1"
                     /translation="AIIKDMEKKAERFLDEQDQKLSHINQTIDEKISRQIVQLVDRGQ
                     LQLDELEKRTEYAIKDAQERMKDQIERARDDFRKMRDDVSREMEVAHAMRDDIMVELE
                     KEKSRMKGLSEKLDVLSRAETLAVKLDETVEVLTDRLKLAEEENSKLDQYVQNFESVR
                     IGRRELEAEIRSLETQRDRIGETEKVIQKMHAQASELKERFGELEQSEAIADRLEERM
                     NDLIEMQADFEKSYAEIGEKRKLLEQSLRMMETSKKQAQDARAAAEQLLASVDRAEFR
                     QNEVDKKILGMESRTVQLSKLEREIDKVESRFMQMEGLMADLEEKQKQIGSMMRKVEE
                     IREHGQEVRTELESTVSEADEKMERLSQLFQTVDRLVDQNFEKAMESSTSVKDRKVKK
                     RSGSDTIKRESILSLHLNHKWDADLIADRLKMDPATVRAIISAS"
     gene            complement(1321..1785)
                     /locus_tag="PKK76_16455"
     CDS             complement(1321..1785)
                     /locus_tag="PKK76_16455"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNL70123.1"
                     /translation="MNFDQNAHPELIGVLGSFTTAKGEFEVRIEGRFDASHYLLKYFP
                     DGSDEPMHGHSWLVEVTLADASGGLREDGLAFDYLAARMRLDKLVDRLDHVVINHLPE
                     FKGINPTTENIARWFYAGLRSEVEGKGGRVIGIRVHEGPANSALFRPSHNQS"
     gene            1878..2099
                     /locus_tag="PKK76_16460"
     CDS             1878..2099
                     /locus_tag="PKK76_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide oxidoreductase"
                     /protein_id="HNL70124.1"
                     /translation="MSDSATKQRFTASMTVGEAMTLHPEAGLVFSSYHLGGCSHCSIN
                     ELETIEQVCAGYGVPVEQLVDSLNNLLDS"
     gene            2343..>2920
                     /locus_tag="PKK76_16465"
     CDS             2343..>2920
                     /locus_tag="PKK76_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70125.1"
                     /translation="MRLQTIAILFFFAAAATGCNKDSGESSEGRGVYSLSSVEGPDKV
                     KALITNDFLKWINDPVIVEAIKKANAKNEKRSMDEIKAADQEWIKAEGISDLMREMLT
                     NDAAQFLKKKQKESDGKYSEIFVMDFQGCNVAMSAKTSDFWQGDEAKFIKSYNDGKGA
                     VFVDKVKFDESTKVPQVQVSLPVLDGNKVIGA"
BASE COUNT          863 a          667 c          779 g          611 t
ORIGIN      
        1 gtgcaatcat caaagacatg gagaaaaagg cagagaggtt cctcgacgaa caggaccaga
       61 aactcagcca catcaatcag accattgacg aaaagatttc gcgtcagatt gtgcagcttg
      121 tagaccgcgg ccagctccag ctggacgaac ttgaaaagag aacagaatac gcaatcaaag
      181 acgctcaaga gcgcatgaaa gatcagatcg agcgagcgcg ggacgatttc cgcaaaatgc
      241 gagatgacgt gagccgcgag atggaagtag cgcatgcaat gcgtgatgac atcatggtgg
      301 aacttgagaa agaaaagagc cgcatgaaag gcctttcaga gaagctggat gttctttcga
      361 gagcggagac gcttgccgtc aaactggatg agactgtaga agttctcaca gatcgtttga
      421 aactagcaga agaagagaac tccaaactcg atcagtacgt gcagaacttc gaatctgtaa
      481 gaattggtcg tcgggaactc gaggcggaga tcagaagcct tgagacccag cgagacagaa
      541 ttggagaaac agaaaaagta atccagaaaa tgcatgctca ggcatccgag ctcaaagagc
      601 gctttggtga actggagcaa tcagaggcaa ttgcggatcg tctggaagaa agaatgaacg
      661 atctcatcga gatgcaggcg gactttgaaa aatcctacgc agaaatcgga gaaaagcgca
      721 agctcctcga acaatctctg cgcatgatgg aaacatccaa gaaacaggcc caggacgcgc
      781 gagcagctgc agaacaattg ctggccagcg tggatcgtgc agaattccgt cagaacgaag
      841 tcgacaagaa gattctggga atggagtctc gcacggttca actttccaaa ctcgagcgcg
      901 aaatcgacaa ggtagagtct cgctttatgc aaatggaagg tctgatggca gacctggaag
      961 aaaagcagaa gcagatcggt tcgatgatgc gcaaggtgga agagattcga gaacatggtc
     1021 aggaagtgag gaccgaactg gaatccacag tgagcgaagc ggatgaaaaa atggaacggt
     1081 tgagccagct cttccagact gtggaccggc ttgtagatca aaacttcgaa aaggcaatgg
     1141 aatcaagcac cagcgtaaag gacagaaagg tcaagaaacg cagcggttca gatacaatca
     1201 aacgcgaaag catcctgagc ttgcatctaa accacaagtg ggatgcggat ctcatcgcgg
     1261 atcgtttgaa gatggatccg gcgactgttc gcgccatcat cagcgcgtcc tgacgctcgc
     1321 ttagctctgg ttatgcgaag ggcgaaacag ggccgaattg gcggggcctt cgtgcacacg
     1381 aatgccaatg acgcggcctc ctttgccctc cacctctgag cgtaatcccg cgtaaaacca
     1441 gcgggctatg ttttcggtgg ttggattgat ccctttgaac tcgggtagat gattgatgac
     1501 tacgtgatcg agcctgtcta caagcttgtc caatctcatc ctggcggcca gataatcaaa
     1561 ggccaggccg tcttcacgca gaccaccgct cgcgtccgcc agtgtgacct ctacaagcca
     1621 ggagtgtccg tgcatgggtt cgtcggaacc atccgggaaa tatttaagca ggtagtggct
     1681 ggcgtcgaac ctgccttcga tgcgaacttc aaattcgccc ttcgcggtcg taaacgaacc
     1741 cagcacaccg atcagttcgg ggtgcgcgtt ttgatcgaag ttcatgcctt acccaactca
     1801 aaataagagt tgaccgtcca ggtttttttc gactttattc tagagcggaa caaaattcaa
     1861 caacaggagg tgatccaatg tctgattctg caacaaaaca aaggtttacg gcaagtatga
     1921 cggtggggga agctatgaca ttgcatcccg aagcaggtct ggttttctca agctatcatc
     1981 tcggtggttg ctcgcattgt tccatcaatg agcttgaaac tatcgagcag gtttgcgctg
     2041 gttatggcgt tcctgtagaa cagctcgtcg acagcctcaa caaccttctg gacagttgat
     2101 gcagcaagag ccgggtatcg cccggctctt gaactcaatt tttgtgtcca gggtttcacg
     2161 atgaaacttc atcgccgcaa tttccatcat caagcaaaag ttctcagtag tcattctcac
     2221 cttttcgctg gcccttcacg tagcttagca gaactttgtt gcaaagaatc acgcccggca
     2281 ttgattcaac acatcggttt tcgcgtggtt gaacaaattc atttcgcagg gaaaagcaaa
     2341 ctatgagact ccaaacaatc gcaattctct tttttttcgc agcggcagcc accggctgca
     2401 acaaagactc tggtgaaagc tcggaaggca gaggcgtata cagcctgtca agcgtcgaag
     2461 ggccagacaa agtcaaggcc ttgatcacaa acgatttcct caagtggatc aacgatcctg
     2521 taatcgtaga agcaatcaaa aaggcgaatg cgaagaatga aaagagatct atggatgaaa
     2581 tcaaagcagc ggatcaggaa tggattaaag cagaagggat ttcagatttg atgagggaga
     2641 tgctgaccaa tgatgcagct cagtttctga aaaagaagca gaaggaatcg gatggcaaat
     2701 acagcgagat ctttgtcatg gattttcagg gatgcaacgt ggccatgagt gcgaagacat
     2761 ctgatttctg gcagggcgac gaagccaaat tcattaagtc ctacaacgat ggcaaaggcg
     2821 cagtcttcgt agacaaagtc aagttcgacg aaagtacgaa agtcccacag gtgcaggttt
     2881 cgctgccagt cctggatggc aacaaagtga ttggtgcaat
//
LOCUS       DAORFF010000506         2903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_23281_length_2903_cov_4.149930, whole genome shotgun sequence.
ACCESSION   DAORFF010000506 DAORFF010000000
VERSION     DAORFF010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23281_length_2903_cov_4.149930"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PKK76_16470"
     CDS             <1..922
                     /locus_tag="PKK76_16470"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNL70126.1"
                     /translation="PAAKSLRDVLDKKSKEFEKIIKIGRTHLMDATPLTLGQEFSGYV
                     SMLSNDIKRLEKVLPGLYELALGGTAVGTGLNTHPKFADLAAKKIAELTKLPFVSAPN
                     KFEALAGHGPLVETHGVLKTLACDLMKIANDVRWLASGPRSGLGELSIPANEPGSSIM
                     PGKVNPTQSEAMTMVAAQVIGNDVAVNFGAASGNFELNVYKPLIIFNTLNSIRLLGDA
                     MNSFREHCAEGIEPVKENIQKHLTNSLMLVTALNPHVGYDAAAKIAKNAFNKNITLKE
                     SAQELGLVSPENFDKWVRPEDMIAPDENVG"
     gene            936..1784
                     /locus_tag="PKK76_16475"
     CDS             936..1784
                     /locus_tag="PKK76_16475"
                     /inference="COORDINATES: protein motif:HMM:NF039714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HNL70127.1"
                     /translation="MRRSILVFALLLVCAGCTKTVILQGYKRADTLLYVRTNNYFDLD
                     GAQSSFVKGKLKEHLSWHRSEELPRVADLLVEIARRGKEKFGHDDALWMESQILGMRD
                     RTLTRIEPDCSEFLTTLNEEQMVNFRAQFAKETSEIEEEFHANQDKKAALRAKRLTDF
                     MEFWTGSLDSAQKAKAQEIARDLPDLTAMRLAYRAHGMAQVERLVREKAGKEKTRDFL
                     RSFWLLPGARPDPVAKQYSNESWKSFRTGTERMDGILTKEQRKKAADKMIELAQDLRK
                     ISLESN"
     gene            1781..2179
                     /locus_tag="PKK76_16480"
     CDS             1781..2179
                     /locus_tag="PKK76_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70128.1"
                     /translation="MKRYSLLILPLFMFCRTYVDIQPPAKGSVENPVLCDQPEGQRRY
                     LRLMRGPDGSPVKFEYLDAVQGPGGHILDRFKVENPARKTDKRSMFSQLWDYIASDPE
                     VPVYFRIYMDLYNPGKLDTEAVPGYKVGAE"
     gene            2176..>2903
                     /locus_tag="PKK76_16485"
     CDS             2176..>2903
                     /locus_tag="PKK76_16485"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNL70129.1"
                     /translation="MMRTLSLFIFTVRLLIISVFVYHGTKPLSAPRRSAARNKVTLWA
                     AKKIATFFIEMGGIYIKAAQFLSTVSNLFDSEFTEVFASVQDKITPRPYAQVRYRFLN
                     EFGSEPESLFQEFDHTAIAAASLGQVHLARTKDGRKVAVKFLHPGIESQIRQDIRALK
                     LAVNLILWVYPYLDFRPHLYEFSTMTLTEIDYENEAENMRRIRDNFANDPRVVVPSVV
                     DELSGGTVLTTEYIEGINISEIEEL"
BASE COUNT          814 a          704 c          714 g          671 t
ORIGIN      
        1 cccggcagca aaaagtcttc gcgacgtgct ggataaaaag tcaaaggaat ttgaaaagat
       61 catcaagatc ggccgcactc atttgatgga tgcaacgccg ctcaccctgg gccaggaatt
      121 ttcaggctac gtttctatgc tttccaacga tatcaagcgt ctggaaaaag ttctgcctgg
      181 actgtatgaa ctggctctgg gtggaaccgc agtgggcacg ggactcaata ctcatccaaa
      241 attcgcagac ctggcagcaa agaaaattgc agaactcaca aagttgcctt ttgtttccgc
      301 tccaaacaaa ttcgaagcac tggcaggcca tggtccactc gtagaaacgc acggagtact
      361 caaaactctc gcctgcgatt tgatgaagat tgcaaacgat gtgcgctggc ttgcgagcgg
      421 tcccaggtcc ggtcttggag aactttctat tcctgcaaat gaaccaggct cttctatcat
      481 gccaggaaaa gtaaatccga ctcaatcaga agcaatgacc atggtggcag cccaggtgat
      541 cggcaatgac gttgctgtaa actttggcgc cgcttccgga aattttgagc tcaacgttta
      601 caaacctctg attattttca atacactcaa ctccatccgc cttctcggtg atgcaatgaa
      661 cagcttccgc gagcattgtg cagaaggaat cgagcctgtg aaagagaaca ttcaaaagca
      721 tctcacaaat tcgctgatgc ttgtaaccgc tctcaatcct cacgttggct atgacgctgc
      781 cgcgaaaatc gcaaagaacg cttttaacaa gaacatcact ttgaaagagt ctgcgcagga
      841 gctgggtctg gtttctccgg agaattttga caagtgggtt cgtccggaag acatgatcgc
      901 cccggacgaa aacgtaggct gaggcattcg tcgtgatgcg tcgcagtata ctggttttcg
      961 ccttactgct cgtatgcgcc ggatgcacga aaacagtaat ccttcagggc tacaaaaggg
     1021 cggacactct tctctatgtc cgcacgaaca actatttcga tctggacggc gcgcagtcgt
     1081 ccttcgtgaa aggaaagctc aaagagcatc tatcatggca cagatctgaa gagcttccga
     1141 gggtggcaga tcttctggtt gaaatcgccc ggcgcggaaa agagaaattt ggtcacgacg
     1201 atgcgctttg gatggaatct caaatactgg gaatgcggga cagaacgctc actcgaatcg
     1261 agccggattg ctcggagttt ttgaccactc tcaacgaaga gcagatggtg aactttcgcg
     1321 cgcaattcgc aaaagaaact tccgaaattg aagaagagtt ccatgcaaat caggataaga
     1381 aagctgccct tcgcgcaaaa aggctgactg atttcatgga attctggact ggaagcctgg
     1441 actctgcgca aaaagcaaag gcacaggaaa tcgcgcgaga cctcccagac ctcactgcga
     1501 tgaggcttgc atacagggcg catggaatgg cacaggtgga aagactcgtg cgcgaaaaag
     1561 caggcaaaga aaaaaccagg gattttctcc gcagcttctg gttgcttccg ggcgccagac
     1621 ccgatccggt cgcaaaacag tattcgaacg agtcatggaa aagctttaga accggcactg
     1681 aaaggatgga cgggattctg acaaaagaac aaagaaagaa agcagcggac aaaatgattg
     1741 agcttgccca ggatcttcgc aagatctcac tggaatcaaa ttgaaacgtt attcgctatt
     1801 gatcctgcca cttttcatgt tctgcagaac gtacgtggat attcaacctc cagccaaagg
     1861 gagcgtggaa aacccggttc tctgcgacca gccggaagga cagaggcgtt atttgcgcct
     1921 gatgagagga ccggacggaa gccccgtaaa gtttgaatac ctggatgcag tccagggtcc
     1981 tggcggccat attctggatc gattcaaagt agaaaacccg gcgcgaaaga cagataagcg
     2041 cagcatgttc tcacaactgt gggattatat tgcaagcgat ccggaagtgc cggtttattt
     2101 cagaatctat atggatctat acaatcccgg caagctagac acggaagctg tccccggata
     2161 caaagtcgga gctgaatgat gcgaactctc tctttgttca tttttacagt tcgacttttg
     2221 atcatttctg tttttgtcta tcacggaaca aaaccgcttt ctgctccacg cagatcagct
     2281 gcgcgaaaca aagtcactct ctgggctgca aagaagatcg caactttctt catagaaatg
     2341 ggtggcatct acatcaaggc cgcacagttt ctttctactg taagtaatct atttgattct
     2401 gaatttacgg aagtctttgc gagcgttcaa gacaagatca ctccgcgtcc gtacgcgcag
     2461 gtacgatatc gttttctaaa tgaattcgga tctgagcctg aatccctttt tcaggaattc
     2521 gatcacacgg cgattgccgc ggcatctctg ggtcaggtgc atctggcccg gacaaaagac
     2581 ggaagaaagg tagccgtaaa gtttctacat ccagggatcg aatctcaaat ccgtcaggac
     2641 attcgcgctc tcaaactcgc cgtaaacttg atcctctggg tttaccctta tctggacttt
     2701 cgccctcatc tctacgaatt cagcaccatg acgctgacgg aaatagacta cgaaaacgaa
     2761 gcagaaaaca tgcgaaggat tcgagataat ttcgcaaacg atccgcgagt agtggttccg
     2821 tccgttgtag acgagttgtc cggcggcacg gtcttaacca cagaatacat tgaaggcata
     2881 aacatttctg aaatagaaga act
//
LOCUS       DAORFF010000507         2892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_23441_length_2892_cov_4.362002, whole genome shotgun sequence.
ACCESSION   DAORFF010000507 DAORFF010000000
VERSION     DAORFF010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23441_length_2892_cov_4.362002"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PKK76_16490"
     CDS             complement(<1..509)
                     /locus_tag="PKK76_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HNL70130.1"
                     /translation="MAEENVLQLELDKLDVSALKRLAALWNINKIPKEKKGIAKKIIE
                     CMQDEFYLKGVLERLSSTQVTIYTSILKSKNSILTLGEIARKIGAPPVNAEMELGVLK
                     RYFLVYQRKNRERLTNNLDKYYFFPESGTRIRMETNEKGQKFRISLPKYLASRGPIPR
                     EWESAAGKKP"
     gene            590..1306
                     /gene="lepB"
                     /locus_tag="PKK76_16495"
     CDS             590..1306
                     /gene="lepB"
                     /locus_tag="PKK76_16495"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNL70131.1"
                     /translation="MLAIRIAAALYSFVFIWGVLREHFNPWVAIAWCGAVILFYLRMR
                     GLALIRRAGTALLFLLHIEYCVCSWMMVRGPSMEPSYHDGDIVFVRKWPYGWILPPLP
                     GFRSAPYVRLSESLPAEGDAVVLRYPGLDPGGDSFLLKRIAALPGEHYRFADGNFIVN
                     GKVRRSISAQPLVVQPPVFSTPDSFSDPFVVYAAANGVPTDGIVPPGFVLVLGDNSAN
                     SRDSRSIGFIPLNFVMGVCK"
     gene            1306..>2892
                     /locus_tag="PKK76_16500"
     CDS             1306..>2892
                     /locus_tag="PKK76_16500"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNL70132.1"
                     /translation="MVKVHNRARIFAGAITIALGLLFARIVFLVVSTDATAVDQKEGI
                     VRGPILDRRGISLARTEEASAIAIAPPEVLDTEFAAQNLSKHLGIPPAEIVEKIYQQS
                     NRRYFYLKHQVDNFTADRIMDLRIPGVHREYEFKRLYPAGRLAGNLLGFVGRDPTTSL
                     SGLERSYHDLLNRAEMGERRGPALYLTIDSLIQSKLEKEMRAAYESSGAKRATGVFMD
                     LDTGEILAMASFPELDPNDYQVSRPDQRENWGIRMAYEPGSTVKVLMAAILLKNNAVR
                     PDEKFFCNGEYKVGNVTVRCRVHGKMFAHGEVTLSDIITKSCNVGIIQAMSRIPPEKL
                     NADLRALGFGERTDLLPPGSGEAPGYLPEIGDWVPSSRIYFPIGQGFSVSPVQLLRAA
                     GSIANGGTLLQPTLARQAVAQNGKILFEHTPIRAKNPFEPAVNREVLRYMRRVVLEGT
                     GRAALISDVAIAGKTGTGQKSSAKGYYDKYVASFLGFFPAQNPRYGGLLIFDEAGEES
                     GGSLAAPVFARFVQSILPVLN"
BASE COUNT          611 a          691 c          802 g          788 t
ORIGIN      
        1 ggtttcttac ccgccgctga ttcccattcg cgcggaatag gcccgcgact tgcgaggtat
       61 ttgggaaggc tgatgcgaaa cttctggccc ttttcatttg tttccatccg gatgcgcgtg
      121 ccgctttccg ggaagaaata gtacttatcc agattgttcg tgagtctttc gcgattcttc
      181 ctctgataga ccaggaagta acgtttgaga acgcccagtt ccatctcggc attgacgggc
      241 ggcgcaccaa ttttacgcgc gatttcaccg agagtaagaa tgctgttctt gcttttaaga
      301 atggacgtgt aaatggttac ttgagtggag ctgagtcttt ccaggacgcc cttgaggtag
      361 aactcatcct gcatgcattc aatgattttc tttgcgatcc ctttcttctc ttttggaatc
      421 ttgttgatgt tccagagagc ggccagcctc ttgagcgcgg acacatcaag tttgtccagc
      481 tcaagctgta agacgttttc ttctgccatg tattgttata ttaaggaaca ggataggggc
      541 acgcctaagg tgcggctgtt gggcctcatg taaagccctt tttgaattga tgctcgccat
      601 tcgaattgct gctgctctct actctttcgt cttcatctgg ggcgtactgc gtgagcattt
      661 caatccctgg gtcgccattg cctggtgcgg ggccgtgatt ctgttctacc tgcgaatgag
      721 agggttggct ctgattcgcc gtgctggaac tgccctgctc tttctacttc acatcgagta
      781 ttgtgtttgc agttggatga tggtgcgggg gccaagcatg gagccttcgt atcacgacgg
      841 cgacattgtg ttcgttcgca aatggccgta tggctggatt ctgcctcccc tacccggatt
      901 tcgttctgcc ccgtatgtgc gcttgagcga atccttacca gcagaggggg acgcagtggt
      961 tttgcgatat cctggtctcg accctggtgg agattctttt ctcttgaagc ggattgcggc
     1021 ccttccgggg gagcattatc gtttcgcgga tggcaatttc atcgtaaacg ggaaggtgcg
     1081 tagatcgatt tcggcgcagc cgcttgtggt gcagccaccc gtattctcca cgccagattc
     1141 attttcagat ccgttcgttg tgtatgcggc ggccaatgga gtgcccacgg acggaatcgt
     1201 accgccaggc tttgtgcttg tactcggaga taactctgcc aattctcgtg attcaaggag
     1261 catcggtttt attccgctca atttcgtgat gggtgtttgc aagtgatggt caaggttcac
     1321 aatcgcgcgc gcatattcgc cggagcgatt accattgcgc ttggactttt gttcgcgcgc
     1381 atcgtgtttc tcgtcgtgtc aacagacgcg actgctgtgg atcagaaaga gggaattgtt
     1441 cgcggtccca ttctggatcg tcgtggtatc agccttgcac gaacagaaga ggcaagtgcg
     1501 attgcaattg cgcctccaga ggtgctcgac actgaatttg cggcgcagaa tctctcaaaa
     1561 cacctgggca tccctcccgc tgaaatagtt gagaaaattt atcaacaaag caacagacgg
     1621 tacttctacc tgaagcacca ggtggacaat tttacggcgg atcgtatcat ggatcttcgc
     1681 atcccgggcg tgcatcgcga atacgaattc aaacgattgt atcccgcagg cagactggcc
     1741 ggaaacctgc ttggttttgt ggggcgagat ccgactactt ctctttccgg gttggaaagg
     1801 tcttaccatg atttgctaaa tcgagcggag atgggagaac gtcgtgggcc cgcgctctac
     1861 ctcacgattg atagtctgat tcagagcaaa ttggaaaagg aaatgcgcgc cgcttacgaa
     1921 tcgtcgggag cgaagagagc aacgggtgta ttcatggatc tggataccgg cgaaattctt
     1981 gcgatggcaa gctttccgga gcttgatccg aatgactacc aggtgagtcg accggaccag
     2041 agggagaact ggggaattcg catggcgtat gagcctggct ctaccgtgaa ggtgttgatg
     2101 gcggccattc tcttgaagaa caatgctgtt cggccggatg aaaaattctt ctgcaatgga
     2161 gaatacaaag ttgggaatgt cacagtgcgt tgtcgcgttc atggcaagat gtttgcgcac
     2221 ggcgaagtaa ctctctctga cataattaca aagtcgtgta acgtaggaat cattcaggcg
     2281 atgagcagaa ttccgccgga aaaactcaat gcggatttgc gtgcgcttgg ttttggagaa
     2341 agaacggatt tgcttccacc cggatcagga gaagctcccg ggtaccttcc tgaaatcggc
     2401 gattgggtgc ccagttcccg aatctatttt ccaatcgggc agggcttcag cgtttctcct
     2461 gtgcagcttc tgcgtgctgc cggttctatt gcaaacggtg gaactcttct tcaaccaact
     2521 ctggcgcgtc aggccgtggc tcaaaatgga aaaattcttt ttgagcatac gcccatccgg
     2581 gcgaagaatc cttttgaacc tgctgtgaat cgagaagtgc tgcgatacat gcgtcgtgtt
     2641 gtgctcgaag gaactggtag ggctgcgctg atttcagatg tggcgatcgc tggaaagact
     2701 ggaacaggac agaagtcgag tgcgaaggga tactatgata agtatgttgc ttctttcctc
     2761 ggtttctttc ccgcacagaa ccccagatac ggcggtcttt tgatttttga tgaagcggga
     2821 gaagaaagcg gcggttctct ggcggcaccc gtgttcgcgc gatttgttca aagcattctt
     2881 cctgttctca at
//
LOCUS       DAORFF010000508         2887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_23516_length_2887_cov_3.594633, whole genome shotgun sequence.
ACCESSION   DAORFF010000508 DAORFF010000000
VERSION     DAORFF010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23516_length_2887_cov_3.594633"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PKK76_16505"
     CDS             complement(<1..471)
                     /locus_tag="PKK76_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70133.1"
                     /translation="MILLIIPFALNFAVTTAGVLALVGLVVLFYVLIVRPAMLIRADR
                     EAPPPEEGSVREIIVDPSLRALKLSIGQIDSDLKTRMNGIREDHIVFRFTKDRELEDY
                     EIQINATGAAQIRVPHSKKMENFVGMERLHSSELIGDSAGFRLVASAREGRVNQY"
     gene            complement(468..1040)
                     /locus_tag="PKK76_16510"
     CDS             complement(468..1040)
                     /locus_tag="PKK76_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70134.1"
                     /translation="MQKKAVLILVTLVACASPQKPVSVNQTTQKVNPADDPFFKEPNP
                     AGETFRVLILNDSYTVRQMSGDTDLVKKQDDSGDKEQLNTFQELAARYDFKDWDFQGL
                     LSLRLHPHRGEIEHIEYVLGQNPKTWQASKLFQEDVSRFQFQFPKGQVTLRDFRVRYR
                     WKIQRRPGLTDEEARKRALEFLKAETLQNH"
     gene            complement(1019..1258)
                     /locus_tag="PKK76_16515"
     CDS             complement(1019..1258)
                     /locus_tag="PKK76_16515"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNL70135.1"
                     /translation="MIETQSGALYTGITTDVQRRFEQHSSGKGARFFRTSKPVRIVHV
                     EPGLDRSTALSREAAIKQLTVHEKRRLIRCKKKRF"
     gene            complement(1298..>2887)
                     /locus_tag="PKK76_16520"
     CDS             complement(1298..>2887)
                     /locus_tag="PKK76_16520"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNL70136.1"
                     /translation="EAEKQFGRDEDAPVAEETEAPSFLFDADNEFTTAPIEAHIAGQK
                     KIDHYLSLFDIAREISAIDDFEEFWDSILFSVMAQLGPETLTIFATRSTMEPGQKLFP
                     VALSGYEGDDEWVLAPGQALYDACLREEGIIYAEQLRTVSEAIALSEMERDIIDRSKA
                     QIIAPLKNGTNMYGIVLIGAAYSAEEYSVDDLEFVSLLGGIAGSGVDRILARLEFDRD
                     TEQLKRRDFFHNRILEAARECSGLRNLDELYDVLIRNLTEHFHVDSYSLVLLNPATQE
                     YSIFAGNRISPESIEKFRLRTTSELAGMVSNFTRVFEVNDFRANGEITACYTNDDLGI
                     MKHYWVVPLIYQNWLVGFFTIHSTTQEWTEFDREMAVSFAELIGPCFGGAIMLGERES
                     LFRDPFSPLEDRLRLELKKSFEFHSHLSLAEIRIKNIKRILTLNPSAQVTQFLSVVGK
                     SIAAHLFHTDFLARIGQGRFAIILPGRGKEESEILIKKIRADFKRLHLLPGSPVDAQF
                     VHSIVSTEDTSDPEKMLAILE"
BASE COUNT          718 a          735 c          711 g          723 t
ORIGIN      
        1 gtactgattg acccgacctt cacgagcact ggcaaccagt cgaaagccgg cagaatcgcc
       61 aatcaattcg ctcgaatgaa gtctttccat gcccacgaag ttttccattt tctttgagtg
      121 tggaactcga atctgcgcgg ctccggttgc attgatctgg atttcgtagt cttccagctc
      181 tctatctttt gtgaagcgaa acacaatgtg gtcttcgcga attccgttca tccgcgtctt
      241 gagatctgag tcaatctgcc cgattgaaag tttcagcgct cggagagatg ggtccacaat
      301 gatctcacga acagatcctt cctctggagg gggagcttct ctgtctgcgc gaatgagcat
      361 cgccggtcta acaatcagta cgtaaaaaag tacaaccagg ccgaccaggg cgagcacgcc
      421 cgcggtggtc acggcaaaat tcagggcaaa aggaatgatg agcagaatca atggttctgc
      481 agagtctcag ctttcagaaa ttcaagtgcc cgcttgcgtg cctcttcatc cgtgaggcct
      541 ggacgacgtt gtatcttcca gcgatagcga acgcgaaaat cacgaagtgt gacctgccct
      601 tttggaaatt ggaactgaaa tctggacacg tcttcctgga agagtttgga cgcctgccag
      661 gtctttggat tctgaccaag tacgtattcg atgtgttcga tttcgcctct gtgaggatga
      721 agacgcagag atagcagacc ctggaaatcc cagtctttga aatcgtaacg cgcggccaat
      781 tcctggaaag tattcagttg ctctttgtcg ccggagtcat cctgcttttt taccagatca
      841 gtatcccccg acatctgacg cacagtgtaa gaatcattca agatgagaac gcgaaatgtt
      901 tcaccggctg gatttggctc tttgaaaaac ggatcatccg ccggattcac tttctgagtc
      961 gtctggttta ctgaaaccgg cttctgtgga gaagcgcacg cgaccagtgt gacaaggatc
     1021 agaaccgctt ttttttgcat cgaatcagcc tccgtttctc atgcacagtg agctgcttga
     1081 tcgccgcttc gcgcgaaagc gcagtgctac gatcgaggcc aggttcgaca tgcacaatgc
     1141 gcacaggttt cgatgtacga aagaagcgcg caccttttcc ggaactgtgt tgttcaaacc
     1201 tgcgttgaac atcggttgta atgccggtgt acagcgcccc gctctgagtc tctatcatat
     1261 agacactcca gctgctcaca gaatgcatgg cgtgatttta ttcgagaatg gcgagcattt
     1321 tttccggatc cgacgtatcc tctgtgctca cgatcgaatg gacgaactgc gcgtctacag
     1381 gcgaacctgg aagcaaatga agtctcttga aatcggcccg gatcttcttg atcagaatct
     1441 ccgattcctc ttttcctctc ccgggaagaa tgatcgcgaa gcgaccctgc ccgatgcgcg
     1501 ccagaaagtc agtatgaaag agatgagcag cgatggattt acccacaaca gacaagaact
     1561 gagtcacctg tgcggaagga ttcaaagtaa ggatgcgttt gatgttcttg atgcgaattt
     1621 cagcaagact caggtgagag tgaaactcga atgatttctt tagttccagg cgaaggcgat
     1681 cttctagagg actgaatgga tcacggaaca gagactcacg ttcgccaagc atgatggcgc
     1741 cgccaaaaca ggggccaatc aattcagcaa aagaaacagc catctcacga tcgaactctg
     1801 tccactcctg tgtggtggag tgaatcgtga agaatccaac gagccagttc tggtagatca
     1861 agggaaccac ccagtagtgc ttcatgatcc ccagatcgtc gtttgtgtag caggctgtga
     1921 tctctccgtt ggcgcggaag tcattcactt caaatacgcg agtgaagttc gataccatgc
     1981 ctgccagttc acttgtggtc ctgagtctga acttctcaat ggactcaggg ctgatgcgat
     2041 tgcccgcgaa aatggaatac tcctgcgttg caggattgag gaggacgagc gaatagctgt
     2101 ccacgtggaa gtgttcggtt agattgcgaa tgagtacgtc atagagctca tctagattcc
     2161 tcaggcctga acattccctg gcagcttcta gaattctatt gtgaaagaaa tctcttctct
     2221 tgagctgctc tgtatctcgg tcaaattcca ggcgcgcaag aattctatcc acacctgaac
     2281 cggcaatccc gccaagaaga gaaacaaact ccagatcatc cacggagtat tcttccgcag
     2341 aataggcagc tccgatcaaa acgatcccgt acatattggt accatttttc agaggtgcga
     2401 tgatttgcgc tttagagcga tcgatgatgt ctctttccat ctcgctgagc gcaattgctt
     2461 ccgatacagt gcgaagctgc tctgcataga tgattccttc ttcgcgcaga catgcatcat
     2521 agagcgcctg accgggcgct agaacccatt catcatctcc ttcgtatccc gaaagtgcga
     2581 ctggaaataa tttctgaccg ggctccattg tgcttcgcgt tgcgaaaatc gtgagagtct
     2641 ccggccccag ctgtgccatc acagagaaca aaatggaatc ccagaattct tcgaaatcat
     2701 caatcgccga gatttcgcga gcgatatcaa acaaagacag atagtgatca atcttcttct
     2761 ggcccgctat gtgagcttca ataggcgcgg ttgtaaattc gttgtccgca tcaaagagga
     2821 aagacggtgc ttctgtttct tccgcaacgg gcgcatcctc atcccggcca aactgtttct
     2881 ccgcttc
//
LOCUS       DAORFF010000509         2886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_23531_length_2886_cov_3.226775, whole genome shotgun sequence.
ACCESSION   DAORFF010000509 DAORFF010000000
VERSION     DAORFF010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23531_length_2886_cov_3.226775"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PKK76_16525"
     CDS             complement(<1..1122)
                     /locus_tag="PKK76_16525"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNL70137.1"
                     /translation="MKRLLIVLVFLQSSAAFALPFIGRVLRTQDGQPIADAKVVILET
                     KKSAFTDEQGAFSLDVPETGYYTFRIIDQDKVYQIRKEIRFENEAIAFYAGDPPKEQP
                     AQETVSDGINVVGKRDRTKLSRYTLSNEEIRRFPGVYGDSLKAVQSLPGISPAVPIGV
                     LPSVNLITSSFLQSATGFSIGPPYSNSGSGFLVLRGAGARASQFYLDGFKILYPFHLG
                     DQSSVLNNDIIRNVDVYIGTYPARYGNATGGVIAITGPDEVKKPTGHINVALFQSDAY
                     YEVPFLDTGYFVGTARQSYPNYALLYLYPAAIPSNAKYSQYNDGQFKMGYKFNNNHTI
                     ETIYFGAHDNLKYTQSVAKASNGGASGLSGVGDSISSVSG"
     gene            1182..1475
                     /locus_tag="PKK76_16530"
     CDS             1182..1475
                     /locus_tag="PKK76_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70138.1"
                     /translation="MHRAALLLMLLFASSCVTDNVVDSYEAQRRINAAVSAKYKQCNL
                     PVSMLILVPFDEQESDVRACEGEILAAACPFSRMPASCFLLLIKKAPAGDVDG"
     gene            1589..2287
                     /locus_tag="PKK76_16535"
     CDS             1589..2287
                     /locus_tag="PKK76_16535"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HNL70139.1"
                     /translation="MPFDPALSYDSILDTIPAVEENGRIDLPYRSEIELVLGKSSSAQ
                     RCRLVHISSTSVYAEDFHGEEKTLPCFDEFTPARPETVRGERRLALEERIKESFPDAV
                     ILRAGGIYGPGRALPLQFLAGQFGRADSGNRMVSRIHVHDLAQLALAAATSDIDLINA
                     VDGNSALNSEVFAYLENLLSVKVPGSWATDLPSGRRIISRYADQLIRFRYPDYRSGFE
                     EILRDADKLPLRES"
     gene            complement(2250..2633)
                     /gene="queF"
                     /locus_tag="PKK76_16540"
     CDS             complement(2250..2633)
                     /gene="queF"
                     /locus_tag="PKK76_16540"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNL70140.1"
                     /translation="MDYESKQDHIRELKTPDIEVFRNIYNDREYRIDMEIPEFTAVCP
                     KTGLPDFGILYLSYKPAEHCLELKSLKEYVLSYRNVGIFHENVVNRILDDIVKACSPR
                     WARLHGDYNVRGGIKTRVTAVYRRP"
     gene            complement(2674..>2886)
                     /locus_tag="PKK76_16545"
     CDS             complement(2674..>2886)
                     /locus_tag="PKK76_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70141.1"
                     /translation="LDDIVHQTSGLSSKAGAAYSDALAQIEQLYKNVGDQIQKLVPPE
                     RINEVKDKIEELTRVIQQRAGGAPKV"
BASE COUNT          650 a          694 c          779 g          763 t
ORIGIN      
        1 gcccgaaacc gagctgatgc tgtctccgac tccgctgaga ccgcttgctc caccgttaga
       61 cgcttttgcc acgctctgtg tatactttag attgtcgtgc gcgccaaaat agatcgtttc
      121 gatagtatga ttgttattga atttgtaacc catcttgaac tgcccgtcgt tgtattgcga
      181 atacttggcg ttagacggaa ttgcggccgg atacaaatag agcagggcat agtttggata
      241 ggattgtcga gcggttccaa caaagtatcc ggtgtccaga aatggaactt cgtagtaagc
      301 gtctgattgg aagagagcaa cattgatgtg gcctgttggt ttttttactt catccggccc
      361 tgtaatggcg atcacgccgc ctgtggcatt tccgtagcgc gctggataag tgccaatgta
      421 gacgtccaca tttcgaatga tatcgttgtt cagtacgctc gattgatccc cgaggtggaa
      481 tggataaagt attttaaacc cgtcgagata aaactgggaa gcccgagcgc ctgctccgcg
      541 aagtacaagg aagccgcttc ctgagtttga ataggggggg ccaatcgaga aacccgtggc
      601 gctttgcaga aaagaagacg tgatcaggtt tacgctcggg agcacaccaa tcggcactgc
      661 aggtgaaata cctggaaggg actggactgc tttaagcgag tctccgtaaa cgcccgggaa
      721 tctgcggatt tcttcgttgg agagagtata gcgggatagc ttggttcggt ctctcttgcc
      781 tactacgttg attccgtcag agacagtttc ctgtgcaggt tgttccttcg gaggatctcc
      841 agcgtagaag gcgatcgctt cgttctcgaa tctgatttct ttgcgaatct ggtatacctt
      901 gtcttgatcg ataatcctaa aggtatagta tccagtctcg ggcacatcga gagaaaatgc
      961 gccctgttcg tccgtgaatg cactcttctt tgtttcgagg atcaccacct ttgcgtccgc
     1021 aatgggttgt ccgtcctgag ttcgcaacac tctgcctatg aatggcagcg cgaatgcagc
     1081 actggactga agaaaaacga ggacaatcag gagtcgcttc atgctgcttg acttacaaaa
     1141 atggatgccg gccctctgcc gcctaggatt tttggaacta gatgcaccgc gccgccctgc
     1201 ttttgatgct gttgtttgct tcttcatgtg taacggataa cgtcgttgat tcgtacgaag
     1261 cccagcgcag aatcaatgca gcggtttcag cgaaatacaa gcagtgcaat ttacccgtga
     1321 gtatgctcat ccttgttcca tttgacgagc aggaatcaga tgtgagagcc tgcgaagggg
     1381 agatcctggc ggccgcctgt ccgtttagta gaatgcccgc ctcctgcttc cttctcctga
     1441 tcaagaaagc gcctgccgga gacgtagacg gttgagagtg ctcgtctgtg gtgctagtta
     1501 cacgggcaaa attctccatc acgagttccc ttccgttgag gtccgatttc tgactcggcg
     1561 gcccgaggcc cttgtttccc aagctttgtt gccctttgat cctgcgttga gttacgactc
     1621 gattctagat acaatccctg cagtcgaaga aaacggtcga attgatttgc cgtacaggag
     1681 tgagatcgaa ctggtccttg gaaaatcttc ctctgcgcaa agatgcagac tggttcacat
     1741 cagttccaca agcgtatatg cggaggattt tcacggggaa gagaaaaccc ttccttgctt
     1801 tgacgaattc actccggccc gaccggaaac cgtaagaggc gagcgcaggc tggcgctcga
     1861 agaaagaatc aaagagagtt ttccggacgc agtgatcttg agggcaggtg gcatctacgg
     1921 tcctggcaga gccctgccgc tgcagtttct ggccggacaa tttggccgtg cagattcagg
     1981 aaacaggatg gtatccagaa ttcacgtgca cgatctggca cagctggcgt tggccgcggc
     2041 cacgtctgat atagatttga tcaacgcagt agacggcaat tccgcgctca actcagaagt
     2101 cttcgcatat cttgagaatc tgctgtccgt gaaagttccg ggaagctggg caacggacct
     2161 gccgtccgga cggcgcataa tcagtcgtta tgcggatcag ttgattcgat ttagatatcc
     2221 ggattaccgc tcaggctttg aggagatact cagggacgcc gataaactgc cgttacgcga
     2281 gtcttgattc cgccacgcac gttgtagtcg ccatgcaggc gtgcccatct cggggaacat
     2341 gcttttacaa tatcatccag gatgcgattg actacgtttt cgtggaagat accaacattt
     2401 ctgtaagaga ggacatattc cttgagactc ttcagctcaa ggcaatgttc cgccggtttg
     2461 taagaaaggt acaggattcc gaaatcagga aggccggtct tcggacagac tgccgtaaac
     2521 tccgggatct ccatgtcgat tctgtattcg cggtcgttgt atatgttacg gaagacttct
     2581 atgtctggcg ttttgagttc gcggatgtgg tcttgttttg attcgtagtc catgaaggaa
     2641 aagctccgct ccgtttccgg agcggattgt ggattaaact tttggagcgc cgccagctct
     2701 ttgctgaatg acacgagtga gttcttcgat cttatctttg acttcgttga ttctttctgg
     2761 tggcacaagc ttctggattt gatcgcctac gttcttgtag agctgctcaa tctgagccag
     2821 tgcgtccgag taggcagcgc ctgctttgga cgaaagtccg gatgtctgat gaacgatatc
     2881 gtcgag
//
LOCUS       DAORFF010000510         2882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_23581_length_2882_cov_3.912628, whole genome shotgun sequence.
ACCESSION   DAORFF010000510 DAORFF010000000
VERSION     DAORFF010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23581_length_2882_cov_3.912628"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PKK76_16550"
     CDS             <1..865
                     /locus_tag="PKK76_16550"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL70142.1"
                     /translation="PRNINPGGLRSFSVSEAVPVPGTVSTNTAGVAFSHSAVNHPTLP
                     MTYVVDTGGNVLVYSRTAAGVFGAPSTFAIGTPTGSYLTISPGARFLFYRANSIIRTA
                     PISSAGTLSTAVGSYTSTGNGYLVSHPDGRFVYVTGTNSIDLLQLGDDGSLTLISQTA
                     TPNLNIPFVIHPNGKYLYMTDSTGTAYVYWINKVTGALLLLQQISVNNSAPQASCIDV
                     RGRFYYTFGSGAGNIATAFRINDTNGTLSQTAAYTLSTSHRGCAFLNAQVSRSHVELR
                     ADEYLPSWLIL"
     gene            931..1593
                     /locus_tag="PKK76_16555"
     CDS             931..1593
                     /locus_tag="PKK76_16555"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNL70143.1"
                     /translation="MRKLQIVLVLIVSSSCTGSFPRTSLDSESQLLLARALFQSSSAT
                     SAAAVPSCASNAFCYTFRTNGLASANLGGISGADATCNSDVNKPAGATGSFKAFLVDP
                     QTNLRRASVTANLGDGQIDWVLRPSKQYRRSDGITILGTTGSNSLFTFALTNSFVSSA
                     GGHWTGLASTWVYDASHCTGWTSTAGSGRDGADNSTSTTAIFNNATLCNTSGPTIICI
                     EQ"
     gene            1710..2402
                     /locus_tag="PKK76_16560"
     CDS             1710..2402
                     /locus_tag="PKK76_16560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNL70144.1"
                     /translation="MSFTQRIFSSSLGRKYLMAISGALLMVFLIAHMTGNLLVFAGPD
                     AFNAYGHYLKTHPQLLWPARIGLIVLFVLHVYTGISLARENRAARPTAYAFKNTIQAT
                     LASRTMALTGLLVLAYLIYHLLHFTIGIVQPQYFSGVDGQGRHDIYTMFILGFRNPII
                     YSSYVLAQIVLVLHLSHASRSFFQSMGWNQARFSGAIKTFSVAYTLVIFLGFLITPTC
                     VILNFIKLPGEL"
     gene            2402..>2882
                     /locus_tag="PKK76_16565"
     CDS             2402..>2882
                     /locus_tag="PKK76_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNL70145.1"
                     /translation="MSLNSNIPSGPLAEKWDKHKFDLKLVNPANKRKFDVIVVGTGLA
                     GASAAATMAELGYNVKVFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSTMRLFYDTIK
                     GGDFRAREANVYRLAQVSANIIDQCVAQGVPFAREYGGTLANRSFGGAQLSRTFYARG
                     "
BASE COUNT          652 a          755 c          710 g          765 t
ORIGIN      
        1 tccgcgcaac atcaatcccg gaggactgcg ttccttttca gtgtctgagg cagtcccggt
       61 gcctggcact gtttctacga atactgccgg agtcgcattt tcccattcag cagtgaatca
      121 tccaactctg ccaatgacct acgtggtcga cactggagga aatgttctgg tttattcgcg
      181 aactgctgcg ggcgtgtttg gtgctccatc tacatttgcc attggtacac cgaccgggag
      241 ttatcttacc atcagtcctg gagccaggtt cttgttctat cgtgctaatt caatcatcag
      301 aactgccccc atcagctctg cgggtactct ttctaccgcg gttggtagct acacttctac
      361 cgggaacgga tatctggtgt ctcatccaga cggaaggttc gtatatgtta cggggacaaa
      421 cagtatcgac ttactgcagt taggtgatga tggttctctg actttgatca gccagaccgc
      481 tacgccgaat ctgaatattc cattcgtgat tcatcccaac ggaaagtacc tgtacatgac
      541 agattcaacg ggtacggctt acgtctattg gattaataag gtgaccgggg cgttgttgct
      601 cctgcagcaa atcagcgtga ataattctgc gccgcaagca tcatgcatag atgtgcgagg
      661 tagattctac tatacttttg gttccggagc aggcaacatt gccactgctt ttcgcatcaa
      721 tgacacgaac ggcaccttgt cgcagactgc agcatacact ctgtccacct cgcatcgcgg
      781 ctgtgcattc ctgaatgccc aggtgagtcg gtctcatgtg gagcttcgcg cggatgagta
      841 tctgccttca tggctgatcc tataaggcaa aaaatgggca taggaattat tgcttgcaca
      901 agtttcgagc gatttgatat gtttagcgtt atgcgtaaac tccagattgt tctggttttg
      961 attgtgtctt ccagttgcac tggaagcttt ccgcgtacat ccctggattc ggagtcgcag
     1021 ctccttctgg cgcgagctct ttttcaaagt tcatctgcaa ccagtgcggc ggcggttcct
     1081 tcatgcgctt ccaatgcgtt ctgctataca tttcggacaa acgggctcgc aagcgcaaat
     1141 ctcggcggca tctccggtgc agatgctact tgcaattcgg acgtgaataa gcccgcgggt
     1201 gcaacaggtt cattcaaggc gtttctcgtg gacccgcaaa cgaatttgcg cagggccagc
     1261 gtgaccgcca atctcggaga tgggcagata gactgggttc tccggccttc gaagcaatac
     1321 agacgatcag acggcattac gattttaggc acgactggtt ccaactcact cttcactttt
     1381 gccctgacaa acagttttgt cagttcggct ggaggacatt ggaccggtct tgcgtcgacg
     1441 tgggtttacg atgcaagtca ttgcacaggt tggacatcca ccgcagggag cggccgcgac
     1501 ggcgcggaca attctacttc caccacagct atcttcaata atgcaacgct ctgcaatacg
     1561 tccggcccaa cgatcatttg tattgagcag taacggaaaa cgccaatttg acggatcttc
     1621 ctgccctcgc cgctctcgtg ccgaaacccc ttgacaaata ggccaagggc gcgagttcta
     1681 aaagaaatag aatctaatcg gaacaaggca tgtctttcac acaaagaatc ttctccagtt
     1741 ccctcgggcg aaaataccta atggcgatct ccggagcgct gctcatggta tttctcattg
     1801 ctcatatgac agggaacctc ctcgtatttg ccgggccaga tgcgttcaac gcttacggcc
     1861 actatctcaa aacgcaccct cagttgctgt ggcctgcgcg aattggactg atcgtccttt
     1921 ttgttctgca tgtgtacact ggcatttcac tcgctcgaga aaatcgagca gcaagaccta
     1981 ctgcgtatgc gttcaaaaat accatccagg caactcttgc atcacgcacc atggctctta
     2041 cagggttgct tgtgcttgct tacctgattt atcatctgct tcacttcacg atcggtattg
     2101 tgcagccaca gtatttctct ggggtagacg gacagggtag acatgatatc tacaccatgt
     2161 tcattcttgg tttcagaaat cctatcatct actcttctta cgtgcttgct cagatcgttc
     2221 tggttctgca tctgagccac gcatctagaa gcttcttcca atccatgggc tggaatcagg
     2281 ccaggtttag cggagcaatc aagacgttct cagtcgcata cacgctcgtc atctttctcg
     2341 gattcctgat cactccgact tgcgtgattc tcaacttcat taaacttcca ggagaactgt
     2401 aatgtctttg aattcaaata ttccgtccgg tcctctggcg gagaaatggg acaaacacaa
     2461 gttcgatctg aaacttgtga atcctgcaaa caaacgcaag ttcgatgtga tcgttgtggg
     2521 gacaggtctg gcgggagctt ctgctgcagc gacgatggcg gaactcggat acaatgttaa
     2581 agtcttctgc ttccaggata gtccacgtcg cgcacactcc atcgctgctc aaggcgggat
     2641 caacgctgcg aagaactacc agaatgacgg cgacagcacc atgcgtctct tctacgatac
     2701 gatcaaaggc ggcgattttc gcgcacgcga agcaaacgtg tatcgcctgg cgcaggtaag
     2761 tgcgaatatc attgatcagt gtgtggcgca gggtgtacct ttcgcacgcg agtacggtgg
     2821 gactttggct aacagatcgt ttggtggcgc gcagctttcg cgtacgtttt atgctcgagg
     2881 tc
//
LOCUS       DAORFF010000511         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_23615_length_2880_cov_3.195752, whole genome shotgun sequence.
ACCESSION   DAORFF010000511 DAORFF010000000
VERSION     DAORFF010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23615_length_2880_cov_3.195752"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="PKK76_16570"
     CDS             complement(<1..1219)
                     /locus_tag="PKK76_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70146.1"
                     /translation="MYYVGIPSRRIRPLWKATAVLCLVTCIRLSLGDAELAAQEFKKF
                     SKEKVDPYAERASRLADVTSFKNYLELGVASLKADWEEEASDTLFKEFTAIDKSAADK
                     AQKDSDKAVASARYDAAELEWENAAQDAIDEELGGFRAQKETVESLSISESEYEAMIA
                     EAEKQAYANPAYDIASFDQSIAQLETDIRSRFDAKLTSDLTTARANNVSLSGKERDAF
                     DQELTRRESDLRTEFNYNENFYIKRARNRYIAVKRQDDFSARLAADSGSAAALGDSII
                     QDALTDLNKNTTDALAQAQQGASTINAQSSDSEISAAGMDWQKRMEAVITVGLTTFEK
                     TEEKLYSERLVWLEETKRSRAEGETIWKQSHDELSRRREAWLTDVQKQIADGRTQWEQ
                     KIAEFSRSREAAEI"
     gene            complement(1207..1812)
                     /locus_tag="PKK76_16575"
     CDS             complement(1207..1812)
                     /locus_tag="PKK76_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70147.1"
                     /translation="MRLYGIALLFVLAGSSLQAQSDPLDAVLSRASGDSSRLTLVNAT
                     DRTLETMVRFEGKFEETLLDARASMREGVESALLNPRAEDSGLETQDLGDLTHSALAL
                     GRMNLPGDSREKKTRSKFRASLRTNMLSRHKEVSLCSRLRPGTSRTSKGRPAARSATL
                     SSNSEDGDGGDGPDPGTVSGLAEQIAPFSENRIPRSILCTT"
     gene            complement(1809..2129)
                     /locus_tag="PKK76_16580"
     CDS             complement(1809..2129)
                     /locus_tag="PKK76_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70148.1"
                     /translation="MMQRVRGFFQSEEKIAWAIACVSLLFCLSVVVAVFTTGSSGRAA
                     ASTNENAKTDLLRQRMLLQARVNLCNLEKKSSTPVCDAGEQSRVVEYKKQIIEIDRKL
                     GESM"
     gene            complement(2126..2665)
                     /locus_tag="PKK76_16585"
     CDS             complement(2126..2665)
                     /locus_tag="PKK76_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70149.1"
                     /translation="MRPVIFVFSILLSACSAYRVESVRSGELQSGAREVAIGFFETYG
                     GRSDMARRNFYDGLSFSLREKGFRPAEAQEIKQLFDRAELPSERNLNPNELVRLASQY
                     PGRLFLQGRIDEVKTETLLEDRIQVMVSVSVYELKRGSKIAEIQVFGKDLEYRTGRES
                     LEMARLVSSELEKMAGLKP"
BASE COUNT          639 a          818 c          658 g          765 t
ORIGIN      
        1 gaatctcagc tgcctccctg gaacgactga attctgcgat cttctgctcc cattgagttc
       61 gcccatctgc gatctgcttt tgcacgtctg tgagccaggc ttcgcgcctt ctggacaatt
      121 cgtcgtgact ctgtttccag atcgtctccc cttccgcgcg cgaacgtttt gtttcttcaa
      181 gccacacgag tcgctcgcta tagagcttct cctctgtttt ctcaaaagta gtcagaccta
      241 cagtgatcac tgcttccatt ctcttttgcc agtccattcc tgcggcagat atctcggagt
      301 cagaagactg tgcattgatc gtggaagcgc cctgctgcgc ctgggccagt gcatctgttg
      361 tgtttttgtt tagatctgtg agtgcgtcct gaatgataga gtcacccaga gccgccgcgg
      421 aaccagaatc cgctgcaagt ctcgcggaga aatcatcctg tcgtttgacc gcaatgtagc
      481 gattgcgagc gcgtttgatg tagaagttct cattgtagtt gaactctgtt cgaaggtctg
      541 attccctccg cgtcagctct tgatcaaatg cgtctcgttc ctttccgctc agagaaacgt
      601 tattcgccct tgcggttgtt agatccgaag tgagctttgc atcaaagcga ctgcggatgt
      661 ctgtttccag ctgagcaatg ctctggtcaa aactggcaat gtcataggcc ggattcgcat
      721 aggcttgctt ttcagcctcc gcaatcattg cttcgtattc actttctgaa atggatagag
      781 actctacagt ttccttttgc gctcgaaagc cacccagctc ttcatcgatt gcatcctggg
      841 ccgcattctc ccattccagc tctgccgcat catagcgggc actggccact gctttgtctg
      901 aatctttctg agctttgtcc gccgctgatt tgtcaatggc agtgaattct ttaaagagag
      961 tgtcagatgc ttcttcttcc cagtccgctt tcaaagaagc aacgcccagc tccagataat
     1021 tcttgaaaga agttacatcc gccaggcgag aggctcgctc cgcatacgga tccactttct
     1081 ctttgctaaa ctttttgaat tcctgtgccg cgagctcggc gtcgcccagg gagagtcgaa
     1141 tgcaggtgac aagacataga actgcagttg ctttccagag cggtctgatc cgccgggatg
     1201 ggattcctac gtagtacata gtatactccg tggaatccgg ttttcagaaa acggagcaat
     1261 ctgctccgca agaccgctta ctgttccggg gtcgggtccg tctcctccgt ctccgtcctc
     1321 gctattggaa gacagagtcg cactccttgc tgcaggacgg cccttgctcg tcctgcttgt
     1381 accgggccga agtctggaac aaagagaaac ctctttgtgc ctggaaagca tgtttgtgcg
     1441 caacgatgcg cggaatttgc ttctggtttt tttttcgcgc gaatctcccg ggagattcat
     1501 tcgacccagg gcaagagcac tgtgcgtcag atcacccagg tcttgcgttt caagacctga
     1561 atcttccgcg cgcggattca atagggccga ctctactccc tctcgcatac tcgcgcgtgc
     1621 atcgagcaaa gtctcttcga atttaccctc aaagcgaacc atcgtttcaa gagtgcgatc
     1681 tgttgcattg accagagtca gtcgagatga atctccactg gcgcgagaaa gcaccgcgtc
     1741 gagtggatcc gactgcgcct gcaaggaaga cccggctaaa acaaaaagaa gtgcaattcc
     1801 ataaagtctc acatgctttc tcccagctta cggtcgattt caatgatctg ctttttgtat
     1861 tctaccacgc ggctctgttc gcccgcatcg cagactggcg tggatgattt cttttccagg
     1921 ttgcatagat tcactcgcgc ttgcaaaagc attctctggc gcagcaagtc tgtcttcgca
     1981 ttttcgtttg tggaagccgc cgctcgcccg gaagaacctg tcgtgaacac ggccaccaca
     2041 acgctcagac aaaacagcaa agaaacacat gcaatcgccc aggcgatctt ctcttcgctt
     2101 tgaaagaatc cgcgcactcg ctgcatcatg gtttcagtcc cgccatcttt tcgagttctg
     2161 aagaaacgag cctggccatt tccagggatt cacggcctgt gcgatactcc aggtccttgc
     2221 caaacacctg gatctcagcg atcttgctgc ctctcttgag ctcgtacaca ctcacactga
     2281 ccatcacctg gatgcgatcc tcaagaagtg tttctgtttt tacttcatca attcttccct
     2341 gaagaaagag tcttcccgga tactgagacg caagacgcac gagctcgttt ggattcagat
     2401 ttctttcaga tggaagctcc gcgcgatcaa agagttgttt gatttcctga gcttccgctg
     2461 gacgaaagcc tttctcgcgc aaagaaaaag acaatccatc gtagaaattg cgacgggcca
     2521 tgtccgatct gccgccataa gtttcgaaaa acccaatggc aacttcgcgc gcgccagact
     2581 gcaattcgcc agagcgaacc gattccactc tgtaagcgct gcatgcggaa agtaaaatcg
     2641 agaaaacaaa aatgaccggt ctcatctgca acacctcact cctgtctgag aggatcttcc
     2701 atctcgcgca aaataatgcg ccgtaaaaca atccgttttt tcccccgtgg caaacgaacc
     2761 accaagagcc atacctctgc cttccacaga atcggacacc cactccattg catttcctac
     2821 aagatcatgg attccaagat ctgatttcag tcccttttac taaggctatc accttttcat
//
LOCUS       DAORFF010000512         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_23664_length_2877_cov_3.459957, whole genome shotgun sequence.
ACCESSION   DAORFF010000512 DAORFF010000000
VERSION     DAORFF010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23664_length_2877_cov_3.459957"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PKK76_16590"
     CDS             <1..612
                     /locus_tag="PKK76_16590"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL70150.1"
                     /translation="ILDGKINRDGDRIEAVVLFSDIRSFTALSEKYEPEKIVKLLNIH
                     FSRVVDVVSKNNGFVDKFIGDAVMAVFDAELTDNMHRLCALKTAAEMLQGVEQTNADL
                     AALGIDPIRIGVGLACGSVIRGNIGSADRREMTVIGDIVNLASRLEAATQELGCPIVA
                     TKDTFDESCKEIQLLRILETRSFIVRGRSQPVDVLTMTMHDPA"
     gene            complement(614..1171)
                     /locus_tag="PKK76_16595"
     CDS             complement(614..1171)
                     /locus_tag="PKK76_16595"
                     /inference="COORDINATES: protein motif:HMM:NF018813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNL70151.1"
                     /translation="MAKGATIFKAEVDIADMDREYYAHHSLTLACHPSETEERMMVRL
                     LAFCMFASDHISFGKGLSTQDEPDLYENDLTGAMNLWLDVGQPDERSIRRSCGKARSV
                     VVVGFGGRGVGIWWDQNKEQLDRLENLTILNLPLKSTQALSSIAARNMNLQCNIQDGT
                     MSIISGDDLIDVEPAVLHGTWPSRN"
     gene            complement(1196..2437)
                     /locus_tag="PKK76_16600"
     CDS             complement(1196..2437)
                     /locus_tag="PKK76_16600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNL70152.1"
                     /translation="MDKRRFEHRAFAALKVATLATVILCNPLSRSLSAEPSCAIGMGT
                     VCVDAVQRGETVTFTAQNRETYEVTITINGNLKNMTSSMRLPYSRTLPGKFNGPILTW
                     KANPRGAWSWTYTFNWTPGSLEGRHDSSVVYDLPFHGAHKIVQGFHGSFSHTGDFEYA
                     VDWEMPVGTSVFAAREGVVIGARSNMNEGGPEERYRNSANFVLIRHSDGSIGSYDHFK
                     QGGVAVRVGDRVERGTLIGYSGNTGFSGGPHLHFFVFRAKDGFTRESFPIKFNTASGV
                     TVPQEGAVNSSPGSSSTEAVETAEQPRTTPGNVTGDLRVCGIVDNSGPRNCKGDYKTT
                     DKIFVYVPIKKPGAYKFRISFTKPFEKSQPVNTEITTEADWGYSYFWITPSEERSPSG
                     TWEATIYVNEHPEGSVRFNAK"
     gene            complement(2466..>2877)
                     /locus_tag="PKK76_16605"
     CDS             complement(2466..>2877)
                     /locus_tag="PKK76_16605"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNL70153.1"
                     /translation="MIIEIKVVSYGMLAVAGVISFALGGVMLVRSGGEVEKATIALIA
                     GSGIAIGSVMAALIYLARKSQNADVQSGAEQMLRSIGMAETVISPSGGRVRVHSELWN
                     ARTEGSEIPEGAKVRILERDGTFLRVERVADKAP"
BASE COUNT          653 a          720 c          728 g          776 t
ORIGIN      
        1 atcctcgacg gcaagatcaa tcgcgatggg gaccggattg aagccgttgt cttattttct
       61 gacattcgtt cttttacggc tttatctgaa aaatacgaac cggagaaaat agtaaaactt
      121 ttaaatattc atttttctag agtagtagat gttgtaagca aaaacaacgg atttgtggat
      181 aagttcatcg gggacgccgt catggcagta tttgacgcag agctcacgga caacatgcac
      241 agactgtgcg cccttaagac ggctgcagaa atgctccagg gagttgaaca aacaaatgca
      301 gatctggcgg ctctgggcat tgatccgatt cgaattggtg ttggtcttgc ctgtggcagc
      361 gtgattcgag gcaacattgg ttccgcagac agacgcgaaa tgaccgtgat cggggacatt
      421 gtcaacctgg ccagtcgcct ggaagcggcc actcaggagt tgggatgtcc gattgttgcc
      481 acaaaggaca cgttcgacga gtcatgcaaa gaaattcagc tgcttcgcat tctggaaaca
      541 agatcgttta ttgttcgcgg aagatcgcaa cctgtagatg tactcaccat gaccatgcat
      601 gatcctgcat gactcagttt ctggatggcc aggttccgtg aagtactgca ggttctacgt
      661 cgatcagatc atctcctgat atgatggaca tggttccgtc ctggatgttg cactgcaggt
      721 tcatgtttct ggcggcgatc gaagagagtg cctgcgtgga ttttagagga agatttagaa
      781 tagttagatt ttccagtctg tccagttgtt ctttgttctg atcccaccag atgcccacgc
      841 cgcgtcctcc aaaacccacg accacgacag agcgcgcttt cccgcacgat ctccggatgc
      901 tcctttcatc tggctgtcca acatcgagcc acaggttcat cgcgcccgtc agatcatttt
      961 cgtagagatc cggttcatcc tgagtggaaa gccctttgcc aaaagaaatg tgatcactcg
     1021 cgaacatgca aaatgcaagc agtcgtacca tcatcctctc ttccgtctct gacggatggc
     1081 atgcgagagt cagagaatgg tgtgcgtagt attcgcggtc catatccgcg atgtccactt
     1141 ctgctttgaa gatcgtggca ccttttgcca ttctatgctc caggtgccag gtttttcatt
     1201 ttgcgttgaa tcgaacggat ccttccggat gctcatttac gtatatggtt gcttcccacg
     1261 ttccggatgg gctgcgttct tctgacggag tgatccagaa ataggaatat ccccagtctg
     1321 cttctgttgt gatttcagtg ttcacaggtt gcgacttttc aaatggcttt gtgaacgata
     1381 tgcggaattt gtatgcgccc ggctttttga tgggaacata tacaaaaatc ttgtctgtag
     1441 ttttgtagtc gcctttgcag tttctcggcc ccgagttgtc tactatgccg cagacgcgaa
     1501 gatctcctgt tacatttccc ggtgtggttc gaggttgctc tgcagtttca acagcttcag
     1561 tagagctgct tccaggagag gaattgactg caccttcttg aggtactgtg acgccggagg
     1621 ctgtgttgaa tttaatagga aacgattctc tcgtgaaccc gtcttttgct ctgaatacga
     1681 agaagtgaag gtgggggcca cccgagaaac cagtgttacc cgaatatcca atcagagttc
     1741 cgcgttctac tctgtcgccg actcgcacag caacgccgcc ctgcttgaag tgatcatagc
     1801 taccgatcga tccgtccgaa tgtcgaatca aaacgaagtt cgcgctgttg cgatatcttt
     1861 cctctggtcc gccttcgttc atattggatc ttgctccgat caccactcct tctcgcgccg
     1921 cgaatacgga agtgcctaca ggcatctccc agtccactgc gtattcgaaa tcccctgtat
     1981 gactgaaaga gccgtgaaac ccctggacga ttttgtgcgc accatgaaaa ggaagatcgt
     2041 atacgacgga agaatcatgc ctgccttcga gagatccagg agtccagttg aacgtgtatg
     2101 tccaggacca ggctccgcga gggtttgctt tccaggtcag gatgggtcca ttgaattttc
     2161 caggcagagt gcgtgagtag gggagacgca tggacgatgt catgttcttg agatttccat
     2221 tgatcgtaat ggtgacctcg taggtttcgc ggttctgtgc cgtgaaagtg actgtctctc
     2281 ctctttgcac tgcatccaca catacagtgc ccattccaat tgcacaggat ggttctgcag
     2341 aaagagaacg cgagagagga ttgcacagaa tgacagttgc aagggtggcc actttgaggg
     2401 ccgcgaaagc gcgatgctcg aatcttcttt tgtccatgcg atgatctcct taaatttttt
     2461 tgaagtcagg gagctttgtc tgcgacccgt tccacgcgca gaaacgtgcc gtctctttcc
     2521 aggatccgta ctttcgcacc ttcaggaatt tcagatccct ccgtgcgcgc gttccagagt
     2581 tcgctgtgca ctcgcactct tcccccggaa ggggagatga cagtctcagc catgccgatg
     2641 gatcgaagca tttgttctgc gcccgattga acatctgcat tctgtgattt cctggcaaga
     2701 tagatgagtg cagccatcac agagccaatg gcgattccgc ttcctgcgat gagtgcgatg
     2761 gttgcttttt ctacttcacc gccggatctc acgagcatga caccaccgag tgcaaaggat
     2821 atgactccgg ccaccgcaag cattccgtag ctgaccactt tgatttctat aatcatc
//
LOCUS       DAORFF010000513         2876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_23675_length_2876_cov_3.886211, whole genome shotgun sequence.
ACCESSION   DAORFF010000513 DAORFF010000000
VERSION     DAORFF010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23675_length_2876_cov_3.886211"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..72
                     /locus_tag="PKK76_16610"
     tRNA            1..72
                     /locus_tag="PKK76_16610"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Arg,seq:ccg)
     gene            75..461
                     /locus_tag="PKK76_16615"
     CDS             75..461
                     /locus_tag="PKK76_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70154.1"
                     /translation="MELVLKYIGIGLGAALAGWGMFHQLIVGGAVSMIKHPDDSEARL
                     FVMSWVAQGGFMSFLGVIPATLLLIYGPSTAAVHTVLSLSALALILLSSHVFLTGFKT
                     HLRPIQIGASLELIYGIYLVILIVLS"
     gene            complement(486..2054)
                     /locus_tag="PKK76_16620"
     CDS             complement(486..2054)
                     /locus_tag="PKK76_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70155.1"
                     /translation="MRKIQVVSLLPLAIASTLAAAFPKFSKDDFSNPSGESAFGIVRS
                     IELDDFAVASVRYLPDGQRVIVGSSDGRVALVDLASARAQWAVSPIANFRDWISVLDV
                     DPTGQKFVFIVSGDQGDAYMTLYIASTQDGRILKKISGEKSNLYQKHFDYEIDLRYPG
                     PDGEKKRLEEDNLAAYWLMVPYAARFTSKGGLITSWKNSADTHAMYDRSFRLFDSNFR
                     KVWEYQVKADEKTWDPLEPAGFKNGLPVPPIVEMTDGSFLFGTPHARVQQITDALAKS
                     NMKKPNLVDQAFAPVFANPKSIHSEFEDTAMPVSDLAVGAAGKVYVSAGDGGQRQIYA
                     FDGKTRREIFHSHEFDASHVQISPNGKLLATGYLTGAGSRVHIIDLERGKLLYMGPRF
                     GSMNTGSVVWNPKFEQAAVASGSILLLSRREKQTVRVGTSWTRTGIFARPGVSICGFG
                     NGAVEITTDPKEGGYSFSESTDFFAGPDGRIAIPAEKAGELYLKAESPGNWTIYGGLK
                     SEEFSRGVKPVLWD"
     gene            complement(2141..>2876)
                     /locus_tag="PKK76_16625"
     CDS             complement(2141..>2876)
                     /locus_tag="PKK76_16625"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNL70156.1"
                     /translation="ILSSKRQVRQSFDGNQLKTGSIKLKQAEEQAGLALSKSIEQYAA
                     KRKAEATVAVNGAEKEIDGIPAGKEEVKTLRDYASAAREALNSATTNYGNQKYEDSIK
                     DSDEAIRLAGIIREQALAAKGKYNVADRNTNITDNKNLGKLPAGWQLYEVQKKKPEDC
                     LSCISQRPQIYGQTRLWTRIYQANKQVIKNPNLIYPGQKLFIPPKTGEIPALNRDEIL
                     TPAKQQPAQPQPMENKTETAPQENMK"
BASE COUNT          671 a          751 c          683 g          771 t
ORIGIN      
        1 gcgcctatag ctcaatggat agagcgcagg tctccggaac ctgaaatcgg ggttcgaatc
       61 cctgtaggcg caaattggaa cttgtcctga aatacattgg aatcggcctt ggagccgcac
      121 tcgctggctg gggaatgttt caccagctca tcgtgggtgg ggccgtaagc atgatcaaac
      181 acccggatga cagcgaagcc aggctctttg tcatgagctg ggtggcgcag ggtggtttca
      241 tgtcttttct aggagtcatt cccgcgactc ttctattgat ctacggccca agcacagctg
      301 cggttcatac agttctttct ctttctgcgc ttgcactcat cctcttatcc agccatgtat
      361 ttctcaccgg attcaaaact cacctcaggc caattcaaat tggcgcaagc ctggaactga
      421 tttacgggat ttatctggtg atcctgatcg ttctctcttg aaaattccag ttagtctacg
      481 ttcgctcaat cccacagaac tggcttcaca ccacgcgaaa actcttcgga tttaagcccg
      541 ccgtaaatcg tccagttccc aggagattct gccttcaaat aaagttcgcc tgctttttct
      601 gcgggaatcg caatcctgcc atccggacct gcgaagaaat cggtggattc gctgaaacta
      661 tacccacctt cctttgggtc cgttgtgatt tctacggcgc catttccgaa tccgcagatg
      721 gatacccctg ggcgcgcgaa gattccagtc ctggtccagg atgtacccac tcgcacagtc
      781 tgcttctctc tgcgcgatag aagcaaaatg gaaccactgg caacagcggc ctgctcaaat
      841 ttcggattcc agaccacaga acctgtattc atgcttccaa agcgaggacc catgtagagc
      901 agctttccgc gctccaggtc tatgatatga acgcgcgatc cagcgcctgt gaggtaaccg
      961 gttgcgagaa gctttccatt cggactgatc tgcacatgcg atgcatcaaa ctcatgcgaa
     1021 tgaaaaattt ctcgtctggt ttttccatcg aaagcataga tctgtctttg accgccatct
     1081 cctgctgaaa cgtaaacttt tccggctgcg cccactgcca ggtccgacac gggcattgca
     1141 gtatcttcaa attcagaatg aatgctcttt ggatttgcaa atactggggc gaaagcctga
     1201 tccaccaggt ttggtttttt catatttgat tttgcaagag catccgtaat ctgctgcacc
     1261 cgcgcgtgcg gagtcccaaa caaaaacgat ccatccgtca tttccacaat cggaggaacc
     1321 ggaagaccat tcttgaatcc tgcaggctca agcggatccc acgtcttctc atctgctttc
     1381 acctggtatt cccagacttt tcgaaaattg gagtcaaaca aacgaaatga acgatcgtac
     1441 atcgcgtgag tgtccgcgct gttcttccag gaagtgatca atccaccctt cgaagtgaag
     1501 cgtgcagcat acggaaccat aagccagtac gcagccagat tgtcctcttc cagacgcttc
     1561 ttctctccgt ctggacccgg ataacgcaga tcaatttcat agtcgaaatg cttctgatac
     1621 agatttgatt tttcgcctga gattttctta agaatgcgac cgtcctgggt agacgcgatg
     1681 tagagagtca tgtacgcgtc gccttgatcg cctgaaacaa tgaagacgaa cttctgaccg
     1741 gttggatcta catccaggac agaaatccaa tctcgaaaat tggcaatcgg agataccgcc
     1801 cactgagcgc gagcgctggc aagatccacc agggccactc taccgtccga tgaaccgaca
     1861 atcactctct gtccgtccgg caagtaccga accgatgcaa cagcaaagtc atccagttca
     1921 atagatcgaa ctatgccaaa tgcgctttca ccggatgggt tggaaaagtc atccttgctg
     1981 aactttggaa atgcagcagc cagagtggac gcgatggcca gaggcagaag agagactacc
     2041 tgaatcttac gcatgccgaa ccataccgtt tgcctggaac caggcaaaca aaaaagcccc
     2101 ggatttccgg ggcttttgcc tacaaaacaa gaaggttgaa ttacttcatg ttttcctgag
     2161 gcgcagtttc tgttttgttt tccattggct gcggctgagc tggctgctgc tttgccggag
     2221 tgagaatttc atcacgattg agagcaggaa tttctccagt ctttggcgga atgaagagtt
     2281 tctggccggg gtaaatcaga ttcgggttct tgatgacctg cttgtttgcc tggtaaatgc
     2341 gagtccagag tctggtctga ccatagatct gcggacgctg cgaaatgcat gagaggcaat
     2401 cctccggttt ctttttctgc acttcgtaga gctgccatcc agcaggaagt ttgcccagat
     2461 tcttgttgtc tgtgatattc gtattgcgat ctgctacgtt gtatttgcct ttcgccgcga
     2521 gagcttgttc acgaatgatc ccagcaagac ggattgcttc atctgaatct ttgatgctgt
     2581 cttcgtattt ttgatttccg tagtttgtgg tcgcggagtt cagagcttcg cgagctgcag
     2641 atgcataatc gcgcagggtt ttaacttcct ctttgcctgc tggaatacca tcgatttctt
     2701 tttcagcgcc attcacagca acggttgctt ctgctttgcg cttggctgcg tactgctcta
     2761 tagatttaga aagtgcaaga cctgcctgct cctctgcttg tttgagcttg atagaaccag
     2821 ttttcagctg attgccatca aagctctgac gaacttggcg cttactggaa agaatc
//
LOCUS       DAORFF010000514         2867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_23818_length_2867_cov_4.764936, whole genome shotgun sequence.
ACCESSION   DAORFF010000514 DAORFF010000000
VERSION     DAORFF010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23818_length_2867_cov_4.764936"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1615
                     /locus_tag="PKK76_16630"
     CDS             137..1615
                     /locus_tag="PKK76_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70157.1"
                     /translation="MFFSVTYALYEAWRIRWIADDAYISFRYILSFISGKGLTFNEGE
                     RVEGYTNFLWTVLLIPFRALGLDLEVVSLVLGCFFFGMLLCAFAFCVRKALSIEFAAV
                     ALLALCAHRHLQIFATSGLETMLFTFLATTGVLLLALKHKAAATEEPERRLAIIAFFS
                     LALATLTRPDGAIIYAVCAGYLFVFEWRQNGPVSAARESVRNHLLSVGILFPVLVAKS
                     LYYGSIVPNTFYAKSASHPYLFQGIRYAALYFESYWPLGIALILATVFWRILPLIRLP
                     VLVVVAYLSFVVFVGGDFMFARFLIPITPLLFYCSVVLLSKIASSRARAVAFAAFVIA
                     IVFRLDPFGGSLSRDGITEENRLYSRSARALLRETALSLRPALIRANPRIAFVGAQAA
                     FAYYWYPLFAIESETGLTDKTIASQSIEQRGKVGHEKNADPEYLRSRHVHIWMRPPPP
                     GFSGPVLQMAGIPGDMGVIEEDPRIFSILTSDDRFVVDGGTH"
     gene            1612..2457
                     /locus_tag="PKK76_16635"
     CDS             1612..2457
                     /locus_tag="PKK76_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNL70158.1"
                     /translation="MKNTGLCLNSAYFGFYAHAGFVHGMVRGGFAPSIVTGCSSGAMI
                     GALYCAGIDMDEVVRLIQKIRKNDFWEGNALTQAAKIFARGVSRYSGLLTGRKIRALL
                     EPYLGGLKFSDLKIPLGVAVSNVTTGRRELCTEGSVLDSVMCSMAFPFLMEIQKMKGN
                     DYIDGGVVDHEPTKEMILDPSVRTIVVHMIETERETPPRSPIVRAFHASLSIIDHETR
                     DLKDRLALARKTKIVRAMTRTPHLGPDRTHYGETALHAGQKSAASILRSLRIPRSRAN
                     GRGLA"
     gene            complement(2435..>2867)
                     /locus_tag="PKK76_16640"
     CDS             complement(2435..>2867)
                     /locus_tag="PKK76_16640"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNL70159.1"
                     /translation="ARTSKKAGKTVTLNYFLVPPGSSGGPGLYLVDLPGYGYATLSHA
                     ERERIRVMVDEYLEATPFRLLVIVLDARRKPAQEEISIRQWCEAHGFRFVFALTKWDK
                     LNNKERSQTLHTWKEFGAPHQPVSNESGYGISEMIKLVRGR"
BASE COUNT          607 a          699 c          751 g          810 t
ORIGIN      
        1 gatgctgcga accgcagcat aaaagagggt gagaaggagc gttgcgattt tgaagcagaa
       61 ctcaagcatt gtcttgaccg caaccatgca cccggtgtcc tttcgcgtgg ccagaaaaat
      121 cggcatatcg cttttgatgt ttttctcagt cacgtatgct ctatatgaag cctggcgcat
      181 ccgctggatt gcggatgatg cctacatttc atttcgttac attctttcct ttatttccgg
      241 gaaaggtcta acgttcaacg aaggcgaacg cgtggagggg tataccaact tcctgtggac
      301 ggttttgctg attccttttc gagcattggg tctcgatctg gaggtcgtgt ctctggttct
      361 cggatgtttc ttctttggaa tgctcctctg tgcgttcgcc ttctgcgtgc gaaaggctct
      421 ttctatcgaa tttgcggccg ttgcccttct ggctctctgc gcacacaggc atttgcaaat
      481 cttcgccact tcaggcctgg agaccatgct ctttacattt cttgcaacta cgggcgtgtt
      541 gcttctcgca ctgaaacaca aagctgccgc tacagaggag ccggaaagac gacttgccat
      601 cattgcattt ttttccctgg ctctggccac tctaactcgt cctgacggag cgatcattta
      661 cgctgtgtgt gccggctatt tgtttgtctt tgaatggcgc cagaacggac cggtgtctgc
      721 agcacgagaa tctgtgcgca atcatcttct ttccgttgga atcctttttc cggttctggt
      781 tgcaaaaagt ttgtactacg gatccattgt acccaatacc ttttatgcca agtcagcatc
      841 tcatccgtat ctttttcaag gaatccgtta tgcggcattg tactttgagt cctactggcc
      901 tctgggcatt gcattgattc tggccaccgt attttggcgc attttgcctt tgattcgact
      961 gcctgttctg gttgtggtcg cctatctttc ctttgtggtg tttgtaggtg gcgattttat
     1021 gtttgcgagg tttctgattc cgatcactcc acttttgttt tactgctccg tggttctact
     1081 atctaaaatc gcctcatcca gagccagggc ggttgctttt gctgcttttg tcattgcgat
     1141 tgtattcagg ttggatccgt tcggcggcag tctgtcgcgc gacgggatta cagaagagaa
     1201 tcgattgtat tctagaagcg ctcgggcgct gctacgggaa actgctctct ctctgcgtcc
     1261 tgctttgatt cgtgcgaatc ctagaatagc gtttgtggga gcgcaggctg cgtttgctta
     1321 ctactggtat ccgttattcg ccattgaatc ggaaaccggc ctcacggaca aaacgattgc
     1381 ttcgcagtca attgaacaaa gaggcaaggt gggtcatgag aaaaatgcgg acccggagta
     1441 cctccgttcg cgtcatgttc atatctggat gaggccacca cctcccggat tttccggccc
     1501 tgtattgcag atggcgggga tccccggaga tatgggtgtt atagaagaag acccgcgtat
     1561 tttctcaatt ctcacatccg atgatcggtt cgttgtcgat ggaggcacac attgaagaac
     1621 acagggcttt gcctgaattc agcctatttt ggcttctatg cacacgctgg attcgtgcat
     1681 ggtatggtgc gcggtggctt tgcgccttcg atcgtcaccg gttgttcctc cggagcgatg
     1741 attggcgcgc tctattgtgc gggaatcgac atggatgaag tggtgcgctt gattcagaag
     1801 attcgaaaaa acgatttctg ggaaggaaac gctctcaccc aggcggcaaa gatcttcgcg
     1861 cgcggagttt cccgttattc gggattgctc acaggaagga agattcgagc acttcttgag
     1921 ccatatcttg gtggactaaa attttcggat ttgaaaattc cactgggcgt tgcggtatcg
     1981 aacgtaacca caggaaggcg agagctttgc acggaaggca gcgtgctcga cagtgtgatg
     2041 tgttcgatgg catttccgtt tctgatggaa attcaaaaga tgaaaggaaa cgactacatc
     2101 gacggcggag tcgtggatca tgagccaacc aaagagatga tcctggatcc atccgtgcgc
     2161 acgattgtgg tgcatatgat tgaaacagag cgcgagactc ctccgcgtag cccgatcgtg
     2221 cgggcattcc acgccagctt atcgatcatt gatcatgaga cgcgtgatct gaaagatcgc
     2281 cttgctctgg cccgcaagac aaagattgtt cgagccatga cgcgcacacc ccatctgggt
     2341 ccggatcgca cgcactatgg tgaaacagct ttgcatgccg gacaaaagag tgcggctagc
     2401 attcttcgct ctttgagaat ccctcgctcg cgggctaacg gccgcggact agcttgatca
     2461 tttcagatat tccgtagcca gattcgttgg atacgggctg atgaggagct ccgaattctt
     2521 tccatgtatg aagagtttgc gatcgctctt tgttgttgag tttgtcccat tttgtaagcg
     2581 caaatacaaa gcgaaatccg tgagcttcgc accactggcg gatggaaatc tcttcctggg
     2641 cgggttttct tctcgcatcg agcacaatga caagcaaacg aaatggagtt gcttccaggt
     2701 attcgtctac catgactcga attcgctcgc gctccgcatg tgaaagagtt gcgtaaccat
     2761 aaccaggaag atccacgagg tacagaccag gtccaccgga gcttccaggt ggaaccagaa
     2821 aataattcag agtgactgtt ttcccggctt tcttggatgt tcgagcc
//
LOCUS       DAORFF010000515         2866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_23840_length_2866_cov_3.504091, whole genome shotgun sequence.
ACCESSION   DAORFF010000515 DAORFF010000000
VERSION     DAORFF010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23840_length_2866_cov_3.504091"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1969
                     /gene="thiC"
                     /locus_tag="PKK76_16645"
     CDS             128..1969
                     /gene="thiC"
                     /locus_tag="PKK76_16645"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNL70160.1"
                     /translation="MQSEIKQNISGPRSKDNPAFSRIYVAGDRYPELRVPFLRVDQSP
                     TQTARGLEPNPAIILYETSGAWGDSAPSIREGHPSPRSKWIIDRGDTVETEARIRPES
                     TVPVFPGRRTRRVAKPGANVSQMHYARKGIVTQEMEFIAIRENTTRAMMRENGNHPGL
                     AKITGQHPGQSFGALTPEITPEFVRDEVARGRAIIPANINHVELEPMIIGRNFLVKIN
                     SNIGTSAVASSIEEEVEKMVWSIECGADTVMDLSTGKHIHETREWIIRNSPVPIGTVP
                     IYEALEKVGGVAEDLTWELYRDTLIAQAEQGVDYFTIHAGVLLRYIPLTANRMTGIVS
                     RGGSILAKWCLSHHRENFLYTHFEEICEIMKAYDVSFSLGDGLRPGSIADANDRAQFG
                     ELETLGELTKIAWKNDVQVMIEGPGHIPMHMIKENMDLQLKHCDEAPFYTLGPLTTDV
                     APGYDHITSAIGAAMIGWYGCAMLCYVTPKEHLGLPDKKDVRDGVIAYRIAAHAADLA
                     KGHPGAQIRDNALSKARFEFRWKDQFNLSLDPATACAFHDETLPAENAKSAHFCSMCG
                     PKFCSMKITEDVRKYAEEKGLAAESALETGLAEKSAEFRDKGAEVYL"
     gene            2046..>2866
                     /locus_tag="PKK76_16650"
     CDS             2046..>2866
                     /locus_tag="PKK76_16650"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNL70161.1"
                     /translation="MLSLINWISDLLKAGAANADLEKTRARIDAVFAAQSERRPHVAR
                     TNAKQRIVKLKAMLQWIYAHRDDIHKALRADFQKPEAETDLSEILVVTSEIKHAISHL
                     RRWMKPRRVGASLTFLTTSSSVQFEPRGVVLILSPWNFPFNLTMGPLVSAVAAGNCVM
                     IKPSEFTPATNAVMRQLISEIFPEEEVAMFEGDKEVATLLLDKPFHHIFFTGSPAVGR
                     VIQTAAAKHLSTVTLELGGKSPVIVDKSVDMADAVKKISWGKFMNNGQTCIAPDYA"
BASE COUNT          783 a          738 c          727 g          618 t
ORIGIN      
        1 tgccgccgat ttgtcctgcg cctatcatcg ctaatttttt tcgtgacata aattccttcc
       61 tggtttttct agaggagtat ctgcggcaaa aacgcttaga aactgccgcg cggagaagct
      121 ttccatcatg cagtcagaga tcaaacaaaa tatttcggga cccaggtcga aagacaatcc
      181 agccttctcg cgcatctacg ttgcgggtga tcggtatccg gaattgagag tacctttcct
      241 tcgcgtggat caatcgccca ctcagacagc gcgcggactg gaaccaaatc cggccatcat
      301 actgtacgag acttcaggag catggggaga ctctgcaccc agcattcggg aaggtcatcc
      361 atcgcccaga tcgaaatgga tcatagatag aggagacacc gtagaaacag aggctcgcat
      421 tcgaccagaa tccacagtac ccgtttttcc aggtcgcaga accagaagag ttgcgaaacc
      481 aggtgcgaac gtttctcaaa tgcactatgc gcgaaaggga atcgtaacac aggaaatgga
      541 attcatcgcc atccgcgaaa acactactcg tgcgatgatg cgcgaaaatg gcaatcaccc
      601 tggtcttgct aaaatcacag gacaacatcc aggccagagc tttggagcgc ttacgcctga
      661 gatcacgcca gaatttgtaa gagacgaagt agctcgcggt cgcgccatca ttccagccaa
      721 catcaatcac gtagaactgg agccaatgat cattggtaga aatttcctcg tgaaaatcaa
      781 ttcgaacatc ggcacgagtg cagtggcctc aagcattgaa gaagaagtag aaaagatggt
      841 ctggtccatt gaatgcggcg ctgacactgt gatggatctg tcgactggaa agcatatcca
      901 cgaaacgcgt gaatggatca tccgcaactc acccgttcca atcggcaccg tgcccattta
      961 tgaagcactc gaaaaagtgg gcggtgtggc agaagatctt acctgggaac tctatcgcga
     1021 tactttgatt gcgcaggcag aacagggagt ggactatttc acaatccacg caggagttct
     1081 tctcagatac attccactca cagcaaatcg catgactggg atcgtaagcc gcggcggatc
     1141 gattcttgcg aagtggtgcc tttcgcatca cagagagaat tttctctaca ctcacttcga
     1201 agagatctgc gaaattatga aagcgtatga tgtgagcttt tcactcgggg acggactgag
     1261 acctggttcc attgcggacg caaacgatcg cgcccaattc ggagagctgg aaacactcgg
     1321 agagctcaca aagatcgcct ggaaaaacga tgtgcaggtt atgattgaag gacccggcca
     1381 tatccccatg catatgatca aagagaatat ggacctgcag ctgaagcatt gcgatgaagc
     1441 tcctttctat actctgggac ctctcaccac agacgtggca cctggatatg accacatcac
     1501 ttctgccatc ggagccgcca tgattggctg gtatggctgt gccatgcttt gctatgtgac
     1561 cccaaaagaa cacctggggc ttccggacaa aaaggatgtc cgggacggtg tgatcgccta
     1621 tcgcatagcg gcacatgcgg ctgatcttgc caaaggccat ccaggagctc agattcggga
     1681 caacgcactc tctaaagcca gattcgaatt cagatggaag gatcagttca atctatctct
     1741 ggatccagcc actgcctgcg ccttccacga tgaaactcta cctgctgaga atgcgaaaag
     1801 cgctcacttt tgcagtatgt gcggcccaaa gttctgctct atgaaaatca ctgaggacgt
     1861 gcgcaaatac gcagaagaaa aaggcctggc cgcagaatcg gcactggaaa ccggtcttgc
     1921 cgaaaaatcg gcagaattca gggacaaagg cgcagaggta tacctctagc ctggggacgc
     1981 gccagagagc agaaacgaaa tactatttca gttgatcctt gcgtttgatc ccacggggat
     2041 gggtgatgct ttcactcatc aactggatat cagacctgct caaggccggc gctgcaaacg
     2101 cagatctgga aaagacccgc gctcgaatcg acgcagtgtt tgcggcacag agtgaaaggc
     2161 gccctcacgt agctcgcacg aacgcgaagc aaagaatcgt caaactcaaa gcgatgcttc
     2221 agtggattta cgcgcacaga gacgacattc acaaagcact tcgcgcagac tttcagaagc
     2281 cagaagcgga aacggatctt tctgagattc tagtggttac atctgagatc aagcatgcca
     2341 tatctcactt gagaagatgg atgaagccca ggcgcgtggg agcatccctt acctttctaa
     2401 ccacaagcag ttccgttcag tttgaacctc gcggtgtggt tctgattctt tccccgtgga
     2461 attttccgtt caatctcacc atgggcccgc tcgtatcggc agtggcggca ggcaactgtg
     2521 tgatgatcaa accgtccgaa ttcactcctg ctacaaatgc agtgatgaga caattgattt
     2581 ccgagatctt tccagaagag gaagtggcga tgttcgaagg agacaaagaa gtggccaccc
     2641 ttctactgga caaaccattc caccacatct tcttcactgg aagtccggcc gtggggcgag
     2701 tgatccagac agcggcagct aaacatctct ccaccgtaac tctagaactc ggcggtaaat
     2761 cgcctgtcat cgtagacaaa agcgtggata tggcggacgc ggtgaagaag atctcctggg
     2821 gcaaattcat gaacaacggg caaacgtgca ttgctccgga ttacgc
//
LOCUS       DAORFF010000516         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_23942_length_2859_cov_3.108417, whole genome shotgun sequence.
ACCESSION   DAORFF010000516 DAORFF010000000
VERSION     DAORFF010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23942_length_2859_cov_3.108417"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="PKK76_16655"
     CDS             <1..1370
                     /locus_tag="PKK76_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNL70162.1"
                     /translation="SEQMDFPIIYCSAKQGFASRELSRAGKEDKDLIPLLDLVLEHVP
                     KAPGDPEAPLQFQIMNMDYDDYLGRLSIGRVFEGRVTKNQDVVLVRGDHKEMHRITKI
                     FGYIGMKRTEVDEAIAGDIVALTGIPDPMIGDTVCHEDHPNPRPPILVDEPTVSMFFS
                     VNDSPFAGKEGQYVTTRQLRERLIREKLTNVALRVIEDPERQDRFQVQGRGDLHLSIL
                     VETMRREGYELQVSRPEVIMRTENGQKLEPFDIVIIDLPEEYSGVVINELNRRKGSMQ
                     GMETSAHGTTRLEYIVPTRGLIGFRSFLITESRGSAAQNSRFLHYAPYAGPIPGRKNG
                     ALVSMEAGTTVAFALWNIQERGSLFVDPGIPVYGGMIIGECAREQDLDVNPAKEKKLT
                     NMRTTSADEAVRLVPPRKLSLEQSLEFIDDDELLEVTPKSLRLRKKLLDPNERKRHAR
                     LAGTT"
     gene            1330..>2859
                     /locus_tag="PKK76_16660"
     CDS             1330..>2859
                     /locus_tag="PKK76_16660"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/class IV aminotransferase"
                     /protein_id="HNL70163.1"
                     /translation="MNVSVMRGWPELHNAVIFEDAGNLPRLFEYPEYVIETALEKDIA
                     SALRKADEASREGLYVAGFLSYDVGQGARGSIFPKPIPRLWLGCYSSFSRASFLEDST
                     SSHYSLKAIPPSMDERDSFMRSVEQIRESIRSGIVYQVNHTRRKLYDMKGSALALYAR
                     LRSLQRARYAAFVSIPDFTVLSLSPELFFSLEDDWIESHPMKGTFSNFISRNEIDADE
                     KSLSENHMILDLVRNDIGRIAEPGSFSLDPARIVQLSTLQQMISVVRARLKKHTGLDQ
                     IFAALFPPGSVTGAPKRTAMQSIDQLEPARGVYTGAIGVVEPFRRSAVFNVAIRTLQI
                     QHGHAVYGTGCGIVWDSVPSSEWAESELKTAWLKPALDDFHIIETMRMDQGRVFLLRA
                     HLRRMQKSAEFFKMDFSVRDALQKISKEKQADRPLRIRLVLYATGIEIKIAPAPPFRI
                     CGISRVIISQDAIHSGDPFRIHKTSERSLYDESLRKANDLGFDDTIFLNETGEVAETA
                     IC"
BASE COUNT          709 a          722 c          726 g          702 t
ORIGIN      
        1 caagcgaaca gatggatttc ccaatcattt attgttctgc gaagcagggt ttcgcttcac
       61 gcgaactttc gcgggcgggc aaagaagaca aagacctcat tccacttctc gatcttgttc
      121 tggagcacgt accaaaagca ccgggcgatc cagaagcacc gctgcaattc cagatcatga
      181 acatggacta cgatgattac ctcggtcgtc tgtcaattgg acgcgtcttc gaaggtcgcg
      241 tcacaaagaa tcaggatgtt gtactcgtcc gcggcgatca taaggaaatg cacagaatta
      301 caaagatctt cggatacatt ggaatgaaac gcactgaagt agacgaagcg atcgcgggcg
      361 atatcgtcgc gctcaccggg attccagatc ctatgatcgg tgacacagtc tgtcacgaag
      421 atcatccaaa tccaagacct ccgattcttg tggatgaacc aactgtttcc atgttcttct
      481 ctgtaaacga ttcgcctttc gcgggtaaag aaggtcagta cgtaaccacg cgtcagctgc
      541 gcgaaagact catccgcgaa aagctcacga acgttgctct tcgcgtcatc gaagaccctg
      601 agcgtcagga tcgttttcag gttcagggtc gcggagatct tcacctgtcc attctggtgg
      661 agacgatgcg acgcgaaggt tatgaactac aggtttcgcg tccggaagtg atcatgcgaa
      721 ctgaaaatgg gcagaagctc gagccgtttg acattgtgat cattgacctg ccagaagaat
      781 acagcggtgt tgtgatcaat gaactcaatc gccgcaaagg atccatgcag ggaatggaaa
      841 cgtctgctca cggcacaact cgtcttgaat acattgtgcc tactcgcggc ctgattggat
      901 ttcgcagctt cttgatcaca gaaagtcgcg gttctgcggc gcagaactct cgctttttgc
      961 actatgctcc gtatgccggt cctatcccgg gaagaaaaaa cggcgctctc gtttctatgg
     1021 aagcaggcac aactgttgct tttgctctct ggaacattca ggaacgcggc agtctttttg
     1081 tagatccggg catcccggtc tatggcggca tgatcattgg cgagtgtgca cgtgagcaag
     1141 atctggatgt aaaccctgcc aaagaaaaga aactgaccaa catgcgcacg acctctgcgg
     1201 atgaagcggt tcgcctggtt ccacctcgca agctcagcct ggaacaatct cttgaattca
     1261 tcgacgatga tgaactactt gaggtgactc caaagagcct tcgccttcgc aaaaagctgc
     1321 tcgatccaaa tgaacgtaag cgtcatgcga ggctggccgg aactacataa cgcagtcatt
     1381 ttcgaagacg cgggcaattt gccgcgtctt ttcgaatatc cagagtatgt cattgagact
     1441 gctctggaga aagacatagc gtccgcactt cgaaaagcag acgaagcatc ccgcgaaggc
     1501 ctgtacgtgg ccgggttcct ttcttacgac gtcgggcagg gcgcccgtgg atccatattc
     1561 ccaaaaccta tccctcgtct gtggctgggt tgttattctt ctttttcgcg tgcgagtttt
     1621 ctagaagatt ctacctcatc gcattacagc ctgaaggcga ttccaccgtc gatggacgaa
     1681 cgagactcgt ttatgcgatc tgtggagcag atccgagaat ctattcgttc tggaattgta
     1741 tatcaggtca atcatactcg tcgcaaactc tatgatatga aggggagtgc tctggctctc
     1801 tatgccagac tgcgatctct gcagcgggcc cgttatgcgg catttgtttc tatccctgac
     1861 ttcactgtct tatcactgtc accggagcta ttcttttccc tcgaagacga ttggatcgag
     1921 agtcatccca tgaaaggtac gttttcgaat ttcatctcca ggaatgagat agatgcggat
     1981 gaaaagagtc tctctgaaaa tcatatgata ctggacctgg tcagaaatga cattggccgc
     2041 atcgcagaac cgggcagctt ttctctggat cctgcgcgca ttgtgcaact cagtactctt
     2101 cagcagatga tttcagtcgt gcgcgctcgt ttaaaaaagc acacgggcct cgatcagatt
     2161 tttgcggcgc tctttcctcc cggatctgtg acaggtgcac ccaaacgaac agctatgcag
     2221 tcgatcgatc aactcgagcc ggcgagggga gtttatacag gcgccatcgg agtcgtagag
     2281 ccgtttcgtc gatctgcagt attcaacgta gccattcgca cgctccaaat ccagcacggc
     2341 catgcggtct acggcaccgg gtgcggaatc gtgtgggatt ctgttccgtc ttctgaatgg
     2401 gcggaatctg agttgaaaac ggcctggctg aaaccagcgt tagacgactt tcacatcatt
     2461 gaaactatga gaatggacca ggggcgcgtt tttcttttgc gcgcacatct tcgaagaatg
     2521 cagaaatcgg cggagttttt caagatggac ttttctgtac gggacgcttt gcagaaaatc
     2581 tccaaagaga agcaagcaga ccgacctctg cgcattcgcc tggtcctgta tgcaacaggt
     2641 atcgaaatca aaattgcgcc tgcgccgccg tttcgaattt gcggaatatc gagagtgatc
     2701 atctcgcagg atgcaattca ttcgggtgat ccgtttcgta tccacaaaac aagcgaaaga
     2761 agcttgtacg atgaatcgct tcgcaaagca aatgatctcg ggttcgatga cacaatcttc
     2821 ctgaacgaaa caggagaggt cgcagagact gccatttgc
//
LOCUS       DAORFF010000517         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_23968_length_2858_cov_2.692116, whole genome shotgun sequence.
ACCESSION   DAORFF010000517 DAORFF010000000
VERSION     DAORFF010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23968_length_2858_cov_2.692116"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PKK76_16665"
     CDS             complement(<1..409)
                     /locus_tag="PKK76_16665"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNL70164.1"
                     /translation="MSPLVQLGVTVLFLQTSPAPQMSLREAEIIAEENHPELRALRQE
                     RAIANENIKVKFRDFFPSVSLSYRQSRTVAQREFDNGSQSIQLNISQPIYDGGRTSLA
                     HEVAKLDASISTEKYKKARNDLKLRVRTAFFALQ"
     gene            complement(411..1031)
                     /locus_tag="PKK76_16670"
     CDS             complement(411..1031)
                     /locus_tag="PKK76_16670"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL70165.1"
                     /translation="MKYIRHNLALVPVFFVFTCAHQELSEQEALQLKQAVTEYREQKF
                     DQAETTLTELGKKFPANIEINVLRARIKYYTRDFAASESILRDLLKKREGNAHASIWL
                     GRVVAVSPKRTEEERLNEASSIFKQVLKDDPENFQAHYLLGRCLEAQGKLELALYEYQ
                     AAEIPLEDQAQRINAHLGDLFTRLNMTDRAAKHRKMAGALAGSQEK"
     gene            1311..1898
                     /locus_tag="PKK76_16675"
     CDS             1311..1898
                     /locus_tag="PKK76_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70166.1"
                     /translation="MIRLLPLIFLVTFCAKPVAGFYSKLHVDDNKGPVIVKSAHYDLH
                     RDSFSYAVASDHVVTMKDALEMPSREKMLLFKRAFLRKAAIQSGLKEGIFDSEEAQQY
                     ILPRLESILEDYYYYEKARPDRIKRSLSSLNVDDESLAALLKEDPALARVASPEKLRH
                     EKDRILERLYELRMAQAKKQAMENLLKTLDPQVVL"
     gene            1895..2482
                     /locus_tag="PKK76_16680"
     CDS             1895..2482
                     /locus_tag="PKK76_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70167.1"
                     /translation="MKFFAVCILFYSASLFAQEFVPGSDVIRESDILRRMEDEIGTPS
                     VSRTAHAAEQKKEPEKPEIPQITVHTGKLSRSGNSTLSQKEIVVSPDESLPVHIPMSD
                     VTEITFMKWTLRAAQDRRYYFPSVCKVRRKDGSEITGKMEYQDWLRLDVGGVSMLSYF
                     VSSTGGAEVEASAVPESVVRSLSFDTSENQESARR"
     gene            2592..>2858
                     /locus_tag="PKK76_16685"
     CDS             2592..>2858
                     /locus_tag="PKK76_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70168.1"
                     /translation="MIAVSGSDHSFYMDRFETGELSPGDYFSVRNQVPAVGMTRDEAE
                     GICKRLEKRLCTMEEWRTACLGVASRSHSYANAFVPGKCNVNGSD"
BASE COUNT          639 a          672 c          747 g          800 t
ORIGIN      
        1 gctgcagagc gaagaaggca gttctgactc gaagcttcag atcgttgcgt gctttcttgt
       61 atttttcggt gctgatggat gcgtccagct ttgccacttc atgggccaga gacgtcctgc
      121 ctccgtcgta gattggttgt gaaatgttta actggataga ctgagagccg ttgtcgaatt
      181 ctctctgcgc taccgttctg ctctggcgat agctcaacga aacagaagga aagaagtctc
      241 tgaattttac cttgatgttc tcatttgcga tagcgcgttc ctgccgcagt gctctgagct
      301 ccggatgatt ctcttctgcg atgatttccg cttcgcgcag actcatctgc ggcgcgggag
      361 acgtctgaag aaaaaggacg gtcaccccaa gttgcacgag cggggacaac ttacttctcc
      421 tgacttccag caagtgcgcc cgccatcttg cgatgctttg ctgcgcgatc cgtcatgttg
      481 agcctggtaa agagatcccc cagatgcgcg ttgattctct gagcctggtc ttcgagcgga
      541 atctccgctg cctggtattc gtaaagcgcc agctccagct ttccctgcgc ctcgaggcat
      601 cggccgagca gatagtgcgc ctggaagttc tcgggatcgt ctttcaagac ttgtttgaag
      661 atggaggatg cttcattcag tctttcttct tccgttctct ttgggctgac cgcaacaacc
      721 cgaccgagcc agatggaagc atgtgcattt ccctctcttt tcttgagtag atctcgcagt
      781 atgctctccg atgcggcgaa atcgcgtgtg tagtacttga ttcgagcacg caatacattg
      841 atttctatat tagcgggaaa tttttttccc agttccgtta gagtagtctc agcctgatca
      901 aacttctgtt cgcgatattc tgtcacagct tgcttgagtt gcaacgcttc ttgctctgaa
      961 agttcctgat gtgcgcaagt gaacacgaaa aagacaggca cgagtgcgag attatgtcgt
     1021 atgtatttca taactatttt gatccgaggc atcggagaaa aaatttcagt gatgtgtgtc
     1081 aaatagattg tgataaaaat cgaaatcgtg cctacgatcc gggagcgttt ggaatgaaag
     1141 gtatgtattt tggaatgaac aacacttgct cttcgtccct gggcgactaa cgttctgttg
     1201 gtttctgttg ccgcgtcttg ttccgaacgt tctttcggat ttttttaatg attcgcgctc
     1261 tttcatcagt ttgtatttga ctgtgttctc tgcttcatcc tcttgccatt gtgattcgat
     1321 tgctccctct catttttctt gtaacattct gcgcaaaacc tgtcgcaggg ttttattcga
     1381 agcttcatgt ggacgataac aaaggtccag tgatcgtcaa atctgctcat tacgatcttc
     1441 atcgtgattc tttcagttac gcagtcgctt cggatcacgt ggtcacaatg aaagatgcat
     1501 tggaaatgcc ttcgcgcgag aagatgcttc tattcaagcg cgcctttttg agaaaggcgg
     1561 ccatacaatc cggattgaaa gaggggattt ttgattcaga ggaagcgcag cagtacattt
     1621 tgccacgtct cgagtccatt ctggaggatt attactacta tgagaaggcg cggccagata
     1681 ggatcaaacg gtctctctct tctttgaatg tagatgatga gtctcttgcc gcacttctaa
     1741 aagaagatcc ggccctggcg cgcgtggcca gtccagagaa attgcgtcat gaaaaggaca
     1801 gaatcctcga gcgattgtac gaacttcgta tggcccaggc aaagaagcag gctatggaga
     1861 acttgctgaa gacattggat ccgcaggtgg tgttatgaaa ttcttcgcag tctgcattct
     1921 gttctactct gcatcattgt tcgcccagga atttgttcca gggtcagatg tgattcgcga
     1981 atcggatatt ttgcgtcgta tggaagatga aatcggcaca ccttccgttt cgagaacggc
     2041 gcacgcagct gagcagaaaa aagagccaga aaagcccgaa attccccaga ttactgtgca
     2101 cacaggcaaa ctgtctcgca gcgggaattc tactctcagc cagaaggaaa tcgtggtttc
     2161 tccggatgaa tcgctgccgg tgcacattcc catgtcggat gtgacagaaa ttactttcat
     2221 gaaatggact ttgcgagccg cccaggatcg caggtactac tttccgtccg tatgcaaagt
     2281 tcgacgcaaa gacggatctg agattacggg caagatggag tatcaggact ggcttcgcct
     2341 ggacgtgggg ggagtttcca tgctatccta ttttgtatct tccaccggcg gcgcggaagt
     2401 agaagcgtcc gccgtacctg aatcagtggt tcgcagtttg tcttttgaca cgtcggagaa
     2461 tcaggaaagt gcgaggcgct gagattttgt tgatactcgc tatgatttct tgcgctctgt
     2521 cgcgcgcgag tgaatcacgc gaacgggctg tggttcgatc tgcggcgtct cccagcgcct
     2581 ggcagactga tatgattgct gtgtctggct ctgatcattc tttttacatg gatcgatttg
     2641 aaaccggaga actatcacct ggagactatt tctctgtcag aaaccaggtt cctgcggtag
     2701 gcatgacccg cgacgaagcg gaaggtattt gtaagaggct cgagaagcgt ctgtgtacga
     2761 tggaggaatg gaggactgcc tgcctcgggg tggcctctcg ttcgcatagc tacgcgaatg
     2821 cgtttgtgcc cggtaagtgc aacgtaaacg gatcggat
//
LOCUS       DAORFF010000518         2843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_24175_length_2843_cov_3.724534, whole genome shotgun sequence.
ACCESSION   DAORFF010000518 DAORFF010000000
VERSION     DAORFF010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24175_length_2843_cov_3.724534"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..392)
                     /gene="rpsP"
                     /locus_tag="PKK76_16690"
     CDS             complement(153..392)
                     /gene="rpsP"
                     /locus_tag="PKK76_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNL70169.1"
                     /translation="MVKIRLQRYGTKKRPFYRLVAAHSTSRRDGRFIEQIGFYDPVSR
                     VEPLRLETERVKHWISKGAIPSQTVESLLKKAGAI"
     gene            complement(422..1075)
                     /gene="rpe"
                     /locus_tag="PKK76_16695"
     CDS             complement(422..1075)
                     /gene="rpe"
                     /locus_tag="PKK76_16695"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004470179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNL70170.1"
                     /translation="MKISPSILAADMLDLKTTLRAMDPSAIDFIHMDVMDGHFVPQLS
                     FGERITELAAQHTKIPLDVHLMVDQPELEGPKYFKLKPHNITFHLEATNFPVRLAREI
                     KSAGILPGIALNPGTSVSLLENVLDEIGMVLIMSVEPGFYGQKFLELAIPKVEQLKKM
                     IGSRDILIEADGGIGEGNIGRLARAGVDIVVAGSSCFAPPDSPSDRARALKSAALNG"
     gene            complement(1072..2085)
                     /locus_tag="PKK76_16700"
     CDS             complement(1072..2085)
                     /locus_tag="PKK76_16700"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNL70171.1"
                     /translation="MKEKPEYQFFVSISRLLLYGALALAIFLSVAFFIFMLRGSASEE
                     FPMPDLVGKYYIDVHNDLARMQIRSVIQKKSYPDKPAGLILAQSIDPGAKVSVREKLY
                     IVVNQPEPVLEMPELVGASYENAKAKLASMPADEEVYALQIGSVSFVPAPNVPAGTVI
                     AQYPAAKERVSPFERAYLLVAQSETAKDMKDAGPIPKQGLLDTIPGQNIAIASQFFVT
                     LKKEYRIRSIKSPASFVESGVVQSAEEKDGVVYLDVLYKKPSERFQNGLEKISVELDE
                     PGVCKGELIRGNETSQFFLTKDRKAAEKTETPLVFYRQGTVQVKVTCGDSVVYKKKFH
                     PEV"
     gene            complement(2098..>2843)
                     /locus_tag="PKK76_16705"
     CDS             complement(2098..>2843)
                     /locus_tag="PKK76_16705"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNL70172.1"
                     /translation="RPVTLKDGGMEGVLQAHDLDLFFVFAYGRILPPSIFELPRHGSV
                     NLHASILPEFRGASPIQASLLAGAKVTGWTLQKIAPDLDAGDILATREVNVDLQDRAL
                     ELTQKLMPAGISLSLECLRNLEALLKSATPQDHARATFCKKITRESARIDWAASAFEI
                     HNLVRAYFPSPVAWTTLGGKNAKILRTMPQELALKSTSPGQIWQEKDRLFVCTGSGWI
                     EILEVQLEGKKALPAADFLKGFRGPPAFV"
BASE COUNT          646 a          726 c          738 g          733 t
ORIGIN      
        1 cgttttttcc aattaccttg cccacatcgc tgtctgcgac gcgcacttcg agcacggatt
       61 cttccggtcc cggaatctcc tcgattttga tctcgtcctt ctttgtgacg agagcttcga
      121 tgatgtattc gagcagaatg gaggcctgca acttagatag ctccggcctt cttgagcagg
      181 ctttctactg tctggctggg gatggcaccc ttgctaatcc agtgcttcac acgctctgtt
      241 tccagacgga gaggctctac acggctcact ggatcataaa aaccaatttg ttcgatgaat
      301 ctgccgtcgc ggcggcttgt tgaatgagcg gctaccagac ggtaaaatgg ccgtttcttg
      361 gtgccgtatc tctgaagtcg tatctttacc acaggtctat tttctgcgcc tgcgtcttat
      421 gtcaaccatt tagagccgca gacttgagcg cacgagctct gtcagagggg gagtccggag
      481 gagcaaagca agaagatccg gccactacta tgtccactcc tgcccgggcc agcctgccaa
      541 tgttgccctc cccgattccg ccgtctgcct caatgagtat gtctctcgag cctatcatct
      601 tcttcagctg ttcgaccttt ggaatggcca gttccaggaa cttttgacca tagaagcctg
      661 gctccacgct catgatgaga accataccaa tctcgtccag gacgttttcc agaaggctca
      721 cagacgttcc aggattcagt gcaatgcctg gcaggattcc agcagactta atttcgcgcg
      781 cgaggcgaac ggggaagttg gtagcttcca gatgaaacgt gatgttgtga ggcttgagct
      841 tgaaatactt gggaccttct agctccggct gatccaccat caggtgaaca tccagaggaa
      901 tctttgtatg ctgtgccgca agttccgtta tgcgctctcc aaaagagagt tgtgggacga
      961 agtgtccgtc catgacatcc atgtgaatga aatcaatggc agatggatcc atggcgcgga
     1021 gagtggtttt taaatccagc atgtccgcgg ccaggatgga aggagagatt ttcatacttc
     1081 tggatgaaat ttctttttat aaacgaccga gtcgccgcag gtgactttca cctgcacggt
     1141 tccctggcga taaaacacga gtggtgtctc tgtcttttct gctgctttgc gatcttttgt
     1201 caaaaagaac tggctggttt cgttcccgcg aatgagttcg cccttgcaaa ctcctggctc
     1261 atcgagctcg actgaaatct tttccaatcc attctggaat cgttcacttg gttttttgta
     1321 gagtacatct aaatagacaa cgccatcttt ttcttctgca ctttgcacta cgccggattc
     1381 cacaaaggaa gctggcgact tgatgctccg gattctgtat tcttttttaa gcgttacaaa
     1441 gaactgcgaa gcaatggcaa tgttctgtcc aggaatcgtg tcgagcaatc cttgctttgg
     1501 aatgggacct gcgtccttca tgtcctttgc ggtttcagac tgcgccacga gtagatatgc
     1561 tctttcaaaa ggagaaactc tctccttggc ggccgggtat tgtgcgatca cagtgccggc
     1621 cggaacattc ggtgccggaa caaaactcac agatccaatt tgcaatgcgt agacttcttc
     1681 gtctgcaggc atacttgcga gctttgcttt cgcgttctcg tacgaagctc ccacaagttc
     1741 aggcatttct agaactggtt cgggctggtt gaccacgatg tagagttttt cgcgcacgct
     1801 gactttggcg ccaggatcaa tgctctgcgc gaggatgagg ccggccggtt tgtcaggata
     1861 actcttcttc tggatcacag aacgaatctg catacgagca agatcgttat gaacatctat
     1921 gtagtactta ccaaccaggt ctggcatggg gaattcttcc gatgcagagc ctctgagcat
     1981 gaagatgaag aaagcaacgg aaaggaagat cgccagtgcc agggctccgt agagaagcaa
     2041 gcgagagatg gagacaaaaa actgatattc tggtttttcc ttcatggttc catccggcta
     2101 gacgaatgca ggcgggccgc ggaatccttt cagaaaatcg gccgcaggca gcgccttctt
     2161 gccttcgagc tggacttcga gtatctcgat ccacccagac ccggtgcaaa caaagagccg
     2221 gtccttttct tgccagattt gcccgggcga cgtgcttttc aatgccaatt cctggggcat
     2281 ggttcgcagt attttcgcgt tcttcccgcc cagagtggtc caggcaaccg gagatgggaa
     2341 ataggcgcgc accaggttat ggatctcaaa ggcagaggct gcccagtcaa tgcgcgcgct
     2401 ttcccgggtg atctttttgc aaaaagtagc acgagcatgg tcctgaggag tggcactttt
     2461 caggagagcc tcgaggtttc ttagacactc gagggaaaga gaaatcccgg caggcatgag
     2521 tttctgtgtg agctcgagag ctctgtcctg taagtctaca ttcacttccc tcgtggccag
     2581 gatgtctcct gcgtccagat caggcgcgat cttttggagt gtccagccgg tcacttttgc
     2641 tcctgccaga agagacgcct ggatgggaga agcgccgcgg aactctggta aaatagaagc
     2701 gtgcaaattg acagatccgt gcctcgggag ttcaaaaatg gaaggaggaa gaattctacc
     2761 gtacgcgaaa acaaagaata aatccaggtc atgagcctga agaacgcctt ccatgccccc
     2821 gtccttgaga gtcacaggtc tat
//
LOCUS       DAORFF010000519         2826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_24401_length_2826_cov_3.474558, whole genome shotgun sequence.
ACCESSION   DAORFF010000519 DAORFF010000000
VERSION     DAORFF010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24401_length_2826_cov_3.474558"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKK76_16710"
     CDS             complement(<1..573)
                     /locus_tag="PKK76_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70173.1"
                     /translation="MMRTLFALLLALTAVQLHAVEPEKDPPVETPKKKGGDPGLEGFK
                     VTGLPLISFSTDDGFGYGVRAYGTYYEEGYAPFKFQAFAQYHKTTRGYEYHEVSLDAL
                     KFLGTPLRLRLDGGFERTLNAQWYGYGNYHDLARIKQIKNGEIPINENTPPNTKRDLY
                     QYDQNITLNNLALTNPFGSGNQIFNPSQRVL"
     gene            complement(581..1195)
                     /locus_tag="PKK76_16715"
     CDS             complement(581..1195)
                     /locus_tag="PKK76_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70174.1"
                     /translation="MRSIFLLLLMFAVFTGCKSGDEAPQETSPLVSLLGLNLVTGYGW
                     TGRLTSQTCSADTIQSTYVSCPVFNGLEAQTVAAVPNNATLVMQLYNNRLSGQFTLIL
                     PHYNFIPTNLYQITTSMTTEQGYDTGSGAVTTVRQAFSNTVPYTVTYTTQSTNTTQVD
                     LLDFAATQDPNYLSGSMKLKFTQPSNAAAGQIIATYGFKFDKLF"
     gene            1390..2319
                     /locus_tag="PKK76_16720"
     CDS             1390..2319
                     /locus_tag="PKK76_16720"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70175.1"
                     /translation="MHHLSDLKGQPRARSILEAYLQKTPPPLLISSGPAGTGKHSAAE
                     IFVQSHFCDTGSACGQCGPCRRMLKGEHPDYIRFPEDRIKIGDRRKPDDFTIRWLIST
                     RLPYRPHEAPFRFVLFPDASLIQDEAETSLLKTLEEPPEHTRFIFLVRDVSELKPTIL
                     SRGLLVPFQLLDRVSLTEVSGVDSPDLLDLLAGSLENLEFFQSELYRSLKSRVADTSS
                     RPGLLELEKWMIDLDRGGAEKMLGAPIESMRLWSLLVLVWLRHLNADVSGNLRRLTAL
                     LDFQGDLRSEMPGFQSYAISRMFHRMACYTEGA"
     gene            complement(2301..>2826)
                     /locus_tag="PKK76_16725"
     CDS             complement(2301..>2826)
                     /locus_tag="PKK76_16725"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNL70176.1"
                     /translation="SYKGIIIGGSVIIEEDGKLYNATPIVSDSKIVDFYRKRNLTQNE
                     TKHLTAGTEAGVFILGGFRFGVLICADVLEPKLIDELAEMGVKLVFAVMNSPYREETV
                     EEKKARDEKLFCAPAAKHGLVIAKCCGVGTTFGRKIQGRSLVVTPTGISWRVSLGEED
                     SELLKTILVQAPSV"
BASE COUNT          649 a          664 c          745 g          768 t
ORIGIN      
        1 caaaactctc tgcgatgggt tgaagatctg gttgcctgaa ccaaacgggt ttgtgagtgc
       61 gaggttgttc agagtgatgt tctgatcata ctgatacaga tctcgtttcg tgtttggcgg
      121 agtgttttcg ttgattggga tttcgccatt cttgatctgc ttgatgcggg cgagatcatg
      181 atagtttcca taaccatacc attgtgcatt gagagttctt tcaaaaccgc cgtccagtct
      241 gaggcggaga ggagtaccca ggaacttcaa agcatcgaga gacacttcgt ggtattcata
      301 gccgcgagtg gttttgtgat actgtgcgaa agcctggaac ttgaagggag cataaccctc
      361 ttcgtagtac gtgccgtagg cgcgaacacc gtatccaaat ccatcatctg tggaaaatga
      421 aatgagaggc agacctgtga ctttgaatcc ttcgaggccc gggtctcctc ctttcttttt
      481 aggagtttct acgggcggat ctttttctgg ttctaccgca tggagctgta cagcagtgag
      541 cgcgaggagt agtgcgaata acgttctcat catgacttcc ttagaatagc ttgtcgaact
      601 tgaagccgta ggtggctatg atctggcccg cagccgcatt ggagggctgt gtgaacttca
      661 gtttcatgga cccggaaaga tagttcggat cctgcgtggc cgcaaagtcg agcaagtcca
      721 cctgcgtggt gtttgtcgat tgcgtcgtgt aggtcacagt gtaaggcacc gtatttgaga
      781 aagcctgacg cacggttgtg accgcaccgg agcctgtatc gtatccttgt tccgtagtca
      841 tggaagtagt gatttgatat aagttggtag gaatgaaatt gtagtgagga aggatcagtg
      901 tgaattgccc ggacaggcgg ttgttgtaca actgcatcac cagagtagca ttgttaggca
      961 ctgccgcgac tgtttgcgcc tccagtccat taaacacagg gcaggatacg tatgtgctct
     1021 ggattgtatc tgctgagcac gtctggctcg tgagtcttcc cgtccatcca tagccggtca
     1081 caagattgag tccgagtaag gatacgaggg ggctggtttc ctgaggtgct tcgtctccgg
     1141 acttgcagcc tgtgaagact gcgaacatca gaaggagcag aaagatgctt cgcatgggca
     1201 cttgaaaacg cacgactgaa aaatcagtca atagaaaagg cgaaattttg atcgggatgt
     1261 tgaaaaaata gtcgtcatgt cccaaaaagg attttactca tcggaccggt gcctgttgtg
     1321 ttagccaaag aaattggggc aggaagagtg taacccggcg ttcgcgccgg gtttcgtttt
     1381 taaactcata tgcatcacct gtcagacttg aaaggccagc ctcgagccag atcgattctg
     1441 gaggcctatc ttcaaaaaac cccgccgcct ttgctcattt cgagtggccc ggccgggacc
     1501 ggaaagcact cggccgcaga gatcttcgtg cagagtcatt tctgcgatac cggatccgcc
     1561 tgtggtcaat gcgggccctg tcgtcgcatg ctcaagggcg aacatcccga ctacattcga
     1621 tttccagaag atcgcatcaa gataggagac aggcgaaagc cggacgactt caccattcgc
     1681 tggttgattt ccacgcgttt gccgtatcgt cctcacgaag cgccctttcg ctttgtgtta
     1741 tttccggatg ccagtctaat tcaagacgaa gcggaaacgt cgttgctcaa gactctggaa
     1801 gaacctccgg agcatactcg tttcatattt ctagtgcgcg acgtaagtga gctcaaacct
     1861 acgattctca gccgcggact tctggtgcct tttcaattgt tggacagagt ttctttgaca
     1921 gaagtatccg gtgtcgattc gcccgatttg ctggatttgc tcgctggcag tcttgagaac
     1981 cttgagttct ttcagtctga attatatcga tcactgaaga gccgagtcgc agatacgtct
     2041 tcgcggccag gtcttcttga gttggaaaaa tggatgatcg atctggatcg tggaggtgcg
     2101 gagaaaatgc tcggggcacc cattgagagt atgcgtctgt ggagtttgct ggtcctggtc
     2161 tggcttcgac acctcaatgc agacgtatcc ggaaatttgc gtagattgac ggcgcttctt
     2221 gattttcagg gagatcttag atcggaaatg cccgggtttc aatcatacgc gatttcgcgc
     2281 atgtttcacc gaatggcctg ttatacggaa ggagcttgaa ccagaattgt ttttagcagt
     2341 tcgctgtctt cttctcccag cgacactctc cagctaatgc ctgtcggagt cacgacaagc
     2401 gaacgtcctt gaattttacg accaaacgtt gttccaaccc cgcaacattt cgcaatcacc
     2461 agtccatgtt tagccgcagg tgcacaaaac agcttctcat cgcgtgcttt cttttcttcc
     2521 acggtttctt cgcgataggg agaattcatg actgcgaaga cgagtttcac acccatttcc
     2581 gccagttcgt ctatgagttt tggttcaagt acgtccgcac atatcagcac gccaaagcga
     2641 aacccgccca gaataaagac gcctgcttct gtcccggccg tgagatgttt agtttcattt
     2701 tgtgtaagat tgcgctttct atagaaatct acaatttttg aatctgagac gatgggcgtt
     2761 gcgttgtaga gttttccgtc ttcttctata atgacgctgc caccaatgat gatccctttg
     2821 taggaa
//
LOCUS       DAORFF010000520         2820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_24498_length_2820_cov_2.907414, whole genome shotgun sequence.
ACCESSION   DAORFF010000520 DAORFF010000000
VERSION     DAORFF010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24498_length_2820_cov_2.907414"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PKK76_16730"
     CDS             <1..532
                     /locus_tag="PKK76_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002743660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNL70177.1"
                     /translation="HCELLFEDVEVPENMILGRVGEGFRLSQERLGPARLTHCMRWIG
                     LARRAMETARTYALEREVFGSKLSDHEGIQWMFAESALEIEQGFMLTLKAAHQLREGA
                     DARHIISMAKWAVSETLCKAIDRAIQITGSLGFSRDLKLELLYRDARAARIADGPSEV
                     HKMVIARNLMNGKVQF"
     gene            598..1557
                     /locus_tag="PKK76_16735"
     CDS             598..1557
                     /locus_tag="PKK76_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011672004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HNL70178.1"
                     /translation="MKSLSGGACQDNYLVDLTVEGGTEKGNHKLVMRTDKGASLFASL
                     SRVDEFHVCDLAYQAGVKTPRPMWLEENPAVLGAPFYFMERITGNANGRFVVKDASIA
                     NARKNLPAELATSLARIHSVKPASCKDEALKQKLGRISGEGKKAIALNSVIELRKETS
                     KLKEPHPALEWILNWLEDHAPETDEPVLIHGDFRTGNFMVSPAGLEGVMDWEFAHWGD
                     RHEDVSWLCMKDWRFGKLNKEAGGFTDRETFYSEYAKASGITLSPEKVRYWEVMGNAR
                     WACGSAGQAERHLSGMDKGIELASIGRRSSEMEFEAMRLIDAV"
     gene            1547..1933
                     /locus_tag="PKK76_16740"
     CDS             1547..1933
                     /locus_tag="PKK76_16740"
                     /inference="COORDINATES: protein motif:HMM:NF040046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HNL70179.1"
                     /translation="MQYRPDAPSLLEAIADFLMKDVLPAVKEHDALAYKTLVSWNMLG
                     VVSRELKSAEPLLNDEIARLLSHFKETRALPESIGEKLELSRELNGRLAEEIRSRSGN
                     NAAQKELVKKALVENLSISNPRFGTE"
     gene            1933..2643
                     /locus_tag="PKK76_16745"
     CDS             1933..2643
                     /locus_tag="PKK76_16745"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNL70180.1"
                     /translation="MPRLYLVRHGRADSTGEVYDRLTDIGHRQAELLADYLVSSGVRP
                     DAVYHGTLQRQRETAEHLTRRRPALSSRFQNTETASLNEFTPEFWGAMSRKLSALDQN
                     FAHDLERYLAIRRKGGRRSMVLFLRLTEAIFESWATGMTPEGCESFDAFENRVLTFTE
                     KLLAHKESDTVVVFSSGTPLSIMIARLLGWDRSRSLDWMRYLQNTSLSVFLPGRAGLS
                     PAAINSLGHLPPDLHTLL"
BASE COUNT          755 a          710 c          752 g          603 t
ORIGIN      
        1 ccactgcgaa cttctctttg aagacgtaga agtgccagaa aacatgatcc tcgggcgagt
       61 gggagaaggt ttcagactca gccaggaaag acttggaccc gcccgcctaa cacactgtat
      121 gcgatggatt ggtcttgcaa ggcgcgccat ggagaccgcc cggacctatg ctctggaaag
      181 agaagtcttt ggctctaaac tttctgatca cgaaggaatc cagtggatgt tcgcagaaag
      241 cgcgctagaa atcgaacagg gattcatgct cactctaaag gcagctcatc aattgcgcga
      301 aggcgcggat gctcgtcaca tcatctccat ggcaaaatgg gcagtcagtg aaacgctttg
      361 caaagcaatc gatcgcgcca tccagatcac aggctctctt ggattcagcc gcgatctgaa
      421 actggaacta ttgtatcgcg acgcaagagc agcgcgcatc gcagacggtc catcagaggt
      481 gcacaagatg gtcatcgcac gaaatctgat gaacgggaag gtgcaatttt gaatctggaa
      541 gaaaaagtag cggactacct cacaagaaga ctgggtgcgc cggctcgcat tgacaagatg
      601 aaatcccttt ccggcggcgc ctgccaggac aactatctgg tggatctgac tgtggaaggc
      661 ggaacggaga aaggaaatca caaactggtg atgcgcacgg acaaaggcgc gtctttgttt
      721 gcaagccttt cgcgcgtcga tgagtttcac gtctgcgatc ttgcctacca ggcaggagtc
      781 aaaacaccca ggcccatgtg gctcgaggaa aaccccgccg tcctgggagc acctttctat
      841 ttcatggaaa gaattacagg gaatgcaaat gggcgattcg ttgtaaaaga cgcatccatt
      901 gcaaacgcta gaaaaaatct tccggctgaa ctggcaacga gccttgccag aattcattct
      961 gtaaaacccg cttcctgcaa agacgaagca ctcaaacaga aactggggcg aatctccggg
     1021 gaaggcaaaa aagcgatcgc tctgaattca gtgattgaac ttcgcaagga aacatcaaaa
     1081 ctcaaagagc cacacccggc cctggaatgg attctaaact ggctcgaaga ccatgcaccg
     1141 gaaacggacg aacctgttct cattcacggc gattttcgca caggcaactt catggtttct
     1201 ccggccggac tggaaggagt catggactgg gaattcgccc actggggaga cagacatgaa
     1261 gacgttagct ggctttgtat gaaggactgg cgatttggca aactgaacaa agaagcaggt
     1321 ggcttcacag accgcgaaac attctactct gaatatgcaa aggcatccgg tatcacactt
     1381 tcccctgaga aagtgcgtta ctgggaggtg atggggaatg cgcgctgggc gtgcggatcg
     1441 gcggggcagg cagagagaca tctgtcggga atggataaag gaatcgaact tgcctccatt
     1501 ggcagaagaa gcagcgaaat ggaatttgaa gcaatgaggt tgatcgatgc agtatagacc
     1561 ggacgctccc tctcttctgg aagcgatcgc agacttcctg atgaaagacg tattgcctgc
     1621 agtcaaagag catgatgctc ttgcttacaa aactctggtc agctggaaca tgctgggagt
     1681 ggtttccagg gaacttaagt ccgccgaacc actgttaaac gatgaaattg cacgccttct
     1741 ttcgcatttc aaggaaacgc gagcactgcc tgaatcgatc ggagaaaagc tggaactctc
     1801 cagagagctc aatggcagac tggcagaaga aatcagatcg aggtcgggaa acaacgcggc
     1861 gcaaaaggaa ctcgtgaaaa aagcgctcgt tgagaacctt tccatatcca atccgcgatt
     1921 tggaacagaa tagtgcccag actctacctt gtgcgacatg gtcgtgcaga ttctaccggg
     1981 gaagtgtatg atcgcctgac ggacattggc cacaggcagg cagagctgct tgcggactat
     2041 cttgtgtcca gtggtgtgag gccagatgca gtgtatcatg gcactctcca gagacagagg
     2101 gaaacagcag agcatctaac aagaaggcgc ccggcactca gcagtcgatt tcaaaacaca
     2161 gagactgcat ctctcaacga gtttaccccg gagttctggg gagccatgtc caggaaacta
     2221 tccgcgctgg accagaattt cgcgcatgac ctggaacgtt atctggccat acgcagaaaa
     2281 ggtggaaggc gatcgatggt tctcttccta cggttgacag aggcaatctt cgaaagttgg
     2341 gctactggaa tgactccaga aggatgcgaa agctttgatg catttgaaaa ccgggttctc
     2401 accttcacag aaaagctgct ggctcataag gagtccgata cagtggttgt tttcagttct
     2461 ggaacacctc tatccatcat gattgcgcga ttgctcggct gggatagatc gcgctccctg
     2521 gattggatga gatatctgca gaatacgtcc ctatctgttt ttcttcctgg tcgtgcgggc
     2581 ctttctcctg ccgccatcaa cagtcttggc caccttccgc cggaccttca cactcttctt
     2641 tgattccttg cttcgctggc cctgacgcaa aatgtcttcc tgcatgcggg cagcgaaaaa
     2701 gcaatagctg cctccgacct cccgcttagc atgaaataac gcggtatcaa cgttcttcaa
     2761 cagctcctca cccgaaaatc cgtcttgcgg cgctattgcc acaccgatac tggcgctgac
//
LOCUS       DAORFF010000521         2817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_24542_length_2817_cov_3.544895, whole genome shotgun sequence.
ACCESSION   DAORFF010000521 DAORFF010000000
VERSION     DAORFF010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24542_length_2817_cov_3.544895"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /gene="murF"
                     /locus_tag="PKK76_16750"
     CDS             <1..1476
                     /gene="murF"
                     /locus_tag="PKK76_16750"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNL70181.1"
                     /translation="GLPWARDFFALAKKYCALFRALHRENIMRDFTQAELESLFGPAS
                     HAYRAAIGVSTDSRTALPGNLFVCLQGDAFDGHNFVAQALEKGVSAIIASEQKRIALQ
                     PVCDQSSVPFYFVSDTLRALQHLAEMARSSLKCKVLAVAGSNGKTSTKDMLFTGASAV
                     RQNVHATAGNLNNHIGLPLTLTNIPSSAGLVILELGMNHKGELAELSKIARPDHAIVT
                     SIAEEHAEFFENIEEIAEAELEVAAGMNGGILLYHAQSAGQEVARRIAESHGLSLKIF
                     SAESPSVDASGIHFTFRGNRLTNSNYLNLVMAENLTGALELLAAAGFTGEELTAAAVA
                     AHPGAARRFEVFRRVSGGREILLIDDSYNANQASFEAGISSLRAVLPHGRLALFAGEM
                     AELGHKSDEAHAQVGIAAARAGYTMLFACGRREAGLMASAYMKHKPDGKVVRGEKPAE
                     LLLAFSADDYDGILVKGSRRARMDIVSDEIKRTGFMGRSYV"
     gene            1469..2575
                     /gene="mraY"
                     /locus_tag="PKK76_16755"
     CDS             1469..2575
                     /gene="mraY"
                     /locus_tag="PKK76_16755"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNL70182.1"
                     /translation="MFEFLYSGGHMPGFFRLFGYVSFRALLAGLFAMAFCFLFGAPII
                     AWLRRLKFGEQIRGDGPQSHLSKAGTPTMGGIMVLASMTAACLLFGNLKNTSFVVTLL
                     CTLSLGAIGFTDDYSKVVLKKKGGMNARMKMLLTLAVALFFCLAYNFMVPAPSVSAKI
                     EYSKTGLFLPFIKGQVIDLTPWFALPFWMVVMVGSAHAVNLTDGLDGLAIGNVCIVAA
                     TLGVLAYITGTPRVANYLNVPAADGAHEIAVFLAALVGAGVGFLWFNAAPAQVFMGDT
                     GSLSLGGAIGMSAILLKKEVLLAIMGGVFVAEALSVMLQVASFKSRGKRIFKMAPLHH
                     HFELSGWPETRVVIRFWLIGVILALISLSTLRIQ"
     gene            2575..>2817
                     /locus_tag="PKK76_16760"
     CDS             2575..>2817
                     /locus_tag="PKK76_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70183.1"
                     /translation="MDLKGKRILVMGASGKSGRSAALCYAKMEATVLLADADAKELPD
                     LAGVVGIQDMRPRDDAGLLDSSVDLIVTAPGVPLSLD"
BASE COUNT          616 a          665 c          803 g          733 t
ORIGIN      
        1 ggcttgccct gggcgcgcga cttcttcgcg ttggcgaaga aatactgcgc gctgtttcgc
       61 gcattacaca gggaaaacat catgcgtgac tttacgcagg cggaactgga atctttgttt
      121 ggtcctgcat cgcatgcata ccgcgcagcg attggcgttt ccacggattc aaggactgct
      181 ttgccgggga atctctttgt ttgtcttcag ggagatgcat ttgacgggca caattttgtg
      241 gctcaggctc ttgaaaaagg agtcagtgcc atcatcgcaa gtgaacagaa gaggatcgcc
      301 ttgcagccag tatgcgatca gtccagcgtt ccgttttatt ttgtttctga cactcttcgc
      361 gcactccagc accttgctga aatggcgaga tcctctttga aatgcaaagt gctcgcagtg
      421 gcaggatcca acggaaagac ttctacgaaa gatatgctat ttacaggtgc ttccgctgtg
      481 aggcagaatg tgcacgcaac tgccggcaat ttgaacaatc acattggact gccattgacc
      541 ctgaccaata tcccgtcttc tgcgggactg gtgattctag aactaggaat gaatcacaaa
      601 ggggagcttg cagagctttc gaaaatcgcg cggccggacc acgcgatcgt cacttccatt
      661 gcggaggagc acgcagaatt ctttgagaac atcgaagaga tcgcggaggc agagctagaa
      721 gtagctgcgg gaatgaatgg tggcattcta ctctatcacg cacagagtgc agggcaggaa
      781 gtggccagaa gaattgctga gtcgcatggg ctgtctctca agatcttttc cgctgaaagt
      841 ccgtctgtag atgcatctgg aattcatttt acgttcaggg gaaatcgcct taccaattcg
      901 aactatttga atcttgtgat ggcagagaat ttgacgggtg ctctagagtt gcttgcggca
      961 gccgggttta ccggggaaga actgactgcg gcagctgtgg ccgctcatcc aggagcagcg
     1021 cgccggtttg aagtgtttcg cagagtctca ggcgggcgcg agatcctgct catagacgat
     1081 tcctacaatg caaaccaggc aagcttcgag gcgggaattt cttctctgcg cgcagttttg
     1141 cctcacggaa gactcgcact gtttgccggg gaaatggcag agctcggcca caagagtgac
     1201 gaggctcacg cacaagtcgg gattgccgcg gcccgggcag gatacacaat gctttttgcc
     1261 tgcgggagac gcgaagcagg gttgatggcg tctgcttaca tgaagcacaa gccggacgga
     1321 aaagtagtga ggggagaaaa accagctgaa ttgctccttg ccttttctgc cgacgattat
     1381 gatggaattc tcgtcaaagg gtcgcgtcgc gcaagaatgg acatcgtaag cgacgagatc
     1441 aaacgcacag ggtttatggg caggtcttat gtttgaattt ctatactccg gcggccatat
     1501 gccgggcttc tttcgtcttt ttggttatgt tagcttccgg gctcttctcg ccgggctttt
     1561 tgccatggcc ttttgctttc tgtttggcgc tcctatcatc gcctggcttc gcagactgaa
     1621 gttcggagaa cagattcgag gcgatgggcc gcagagtcat ctgagcaaag caggcactcc
     1681 cactatggga ggcatcatgg ttttggccag tatgactgct gcctgtctct tgtttggaaa
     1741 cttgaagaac acttctttcg tcgtgacttt gctttgcact ctatctctcg gtgcgattgg
     1801 ctttacggac gattacagca aggtagtact caagaaaaaa ggtgggatga atgctcgcat
     1861 gaagatgttg ctcactctgg ccgtggcatt gttcttctgt ctggcataca acttcatggt
     1921 tcccgctccc agtgtaagtg cgaagattga atactcaaag acaggattgt ttttgccttt
     1981 catcaaagga caggtgattg atctaacacc gtggtttgct cttcctttct ggatggtagt
     2041 gatggttggc tcggcccatg ctgtgaatct cacagacgga ctggacggtc ttgccattgg
     2101 aaacgtttgc attgttgcgg caacgttagg cgtccttgct tacatcaccg gaacacccag
     2161 agtggccaac tacctcaacg ttcctgccgc ggacggagct catgagatcg ccgtgtttct
     2221 ggccgcactc gtaggtgcgg gtgtaggatt tttgtggttc aacgcggcgc cggcacaggt
     2281 cttcatggga gatacaggtt cgttgtctct gggcggtgcg ataggaatgt ctgcgatttt
     2341 gctcaagaag gaagttctac ttgctatcat gggtggcgtg tttgtagcag aggcgttgtc
     2401 cgtaatgctt caggtggcga gcttcaaaag cagaggaaaa agaatcttca agatggcacc
     2461 tcttcatcat cattttgaat tgtctggctg gcctgagacc agggtggtca ttcgattctg
     2521 gctcattggc gtgattctgg ctttgatttc actttctact ttgcgcatcc agtaatggat
     2581 ctaaaaggaa aaagaattct ggtgatgggc gcgtccggta aatccggaag atcagctgcg
     2641 ctctgttatg cgaagatgga agcaacggtt ctcctggcgg atgcggacgc aaaggagttg
     2701 ccggacctgg cgggcgtggt tggaatccag gacatgcgtc cccgcgacga tgcaggactg
     2761 cttgattcct cagtggatct catcgtcacg gcacctggtg ttcccctcag ccttgat
//
LOCUS       DAORFF010000522         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_24766_length_2803_cov_3.796579, whole genome shotgun sequence.
ACCESSION   DAORFF010000522 DAORFF010000000
VERSION     DAORFF010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24766_length_2803_cov_3.796579"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PKK76_16765"
     CDS             complement(<1..280)
                     /locus_tag="PKK76_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70184.1"
                     /translation="MSRGAAFFVRILLLACGFTVASCLGHSGRGESDLTPFGLMAGLG
                     LRSGMPAATNASTAPSVDLPPGANLPEEAIQVTESQVELPAQIDGTFVP"
     gene            283..1854
                     /gene="mgtE"
                     /locus_tag="PKK76_16770"
     CDS             283..1854
                     /gene="mgtE"
                     /locus_tag="PKK76_16770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNL70185.1"
                     /translation="MTSKNLDGPLLSSRQLHSEPGQFILAERNPSVKKGEWTIPAAVP
                     EKVPSRAFDFRRTTMQNAEDFVDELEELIESAPHQVKTFFAQWNIHDIAELLNQLHLS
                     EAASALRLLPAEVTLSLMNLPDLKRRGPILEDINHLDTAAAAAILEGIASDQRAAIAR
                     QITPHQQRRMLSALSPETRAQVESSLRYPEHSAGSLMSTEYVRLLPGVTVGQVLSYIR
                     SVAREKDTIYACFIIEPETERLLGAVSLRDLVMAELDWPIEKVMRRKPVHVHVREDRE
                     SVARKISKYNLLAVSVLDEDGLMVGFITVDDVIDVMVEEQTEDILKMGAVGSEGLDTP
                     YMKTGFFEMVRRRATWLVILFLSEMLTATAMGFFEDEIAKAVVLALFVPLIISSGGNS
                     GSQATTIIIRALALGEMHLRDWLRVMFREIRSGLALGTILGVIGFLRIAVWSMFPSIY
                     GPHWILVALTVGTSLVGIVLWGCVAGAMLPFLLKRVGLDPATSSAPFVATLVDVTGLI
                     IYFSVAFLIMRGTLL"
     gene            1955..>2803
                     /locus_tag="PKK76_16775"
     CDS             1955..>2803
                     /locus_tag="PKK76_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70186.1"
                     /translation="MQNEGLGYLWHQFTWDENLPGLILFGVLAVVFALLILYWRYRQK
                     EARLWERLGGLAVRRQLSAEELGLLETFYAALPRPNQELVLKDTKRFWQLLFRFSRAQ
                     MRTHPLEMIRLINKLFPVARFQWNITAIEDVQPGEMVAVQLAKQNLIARVALVNRDQK
                     RMELAFADKNFEVADSISASLYFFRPGLGETRMRGSLYAASRGHALFQLEGDKIEVSG
                     QQKLMARAELSFSMSPIAEARDEGKSETLLTGRTEYVSPRALMIQLDAPALYGTAEKK
                     TDAWKMD"
BASE COUNT          694 a          728 c          745 g          636 t
ORIGIN      
        1 caggaacgaa tgttccatca atttgagccg ggagctccac ctgggattcc gttacctgga
       61 tcgcctcttc tggcaaattc gcaccagggg gcaagtcaac ggaaggcgcg gtgctggcat
      121 ttgtagcagc cggcatcccg ctacgaagac cgaggcctgc catcaaaccg aatggagtca
      181 aatcagactc tccgcggcct gaatgcccaa ggcaactagc aaccgtaaac ccgcacgcaa
      241 gcagaagaat tcgcacaaaa aatgccgcgc cacgtgacat agatgacctc gaagaatctg
      301 gatggaccat tgctctccag caggcaactc cactctgagc ccggtcaatt cattttggct
      361 gaaagaaacc cttcggtcaa aaaaggagaa tggacgattc cggccgctgt ccctgaaaaa
      421 gtgcccagtc gggcgttcga ctttcgtaga accaccatgc agaatgcgga agatttcgta
      481 gatgaattag aagaattgat cgagtctgcg cctcatcaag ttaagacttt ctttgcgcag
      541 tggaacatcc atgacattgc ggaactcctc aatcagctcc atctttctga ggccgcgtct
      601 gcactgagac ttctgcccgc cgaagtcact ctcagcctga tgaaccttcc ggatctaaaa
      661 agacgcgggc ctattctgga agacatcaat catcttgata ctgccgcggc cgcagcaata
      721 ttagaaggca tcgcatcgga ccagcgtgct gccattgcac gccagatcac tccacaccag
      781 caacgtagaa tgctgtctgc tctgagtcca gaaacgcgcg cgcaagtgga atcttctctt
      841 cgctatcccg aacattctgc cggcagtctc atgagcacgg aatacgtccg cctgttaccg
      901 ggtgtaaccg tgggccaggt gctcagctat attcggtccg ttgcccggga aaaagacacg
      961 atctacgcgt gcttcatcat tgaaccggag actgagagat tgctcggcgc agtttcattg
     1021 cgcgatctgg tgatggcgga actggattgg cccatagaaa aggtgatgag gcgcaaaccg
     1081 gttcatgttc atgttcgcga agatcgcgag tccgtcgcgc gcaagatcag caagtacaat
     1141 ctgctcgccg tatcagtgtt agacgaagat ggtctgatgg tgggattcat aaccgtagac
     1201 gacgtcatcg atgtgatggt ggaggagcaa acagaagaca tcctcaaaat gggagctgta
     1261 ggatcagaag gactcgacac cccgtatatg aagactggct tttttgaaat ggtgaggcgt
     1321 cgcgcaacgt ggctcgtgat tctgttccta agcgaaatgc tcaccgcaac tgccatggga
     1381 ttctttgaag atgaaatcgc aaaagcagtc gtgctcgctt tgtttgtgcc tctgatcatt
     1441 tcaagcgggg gcaactcggg atcacaggca accactatca tcattcgcgc gcttgctctt
     1501 ggtgaaatgc atctacgcga ctggctgcga gtgatgtttc gcgaaatccg atcggggctc
     1561 gcactgggca cgattcttgg cgtaataggg tttctaagaa ttgcagtctg gagtatgttt
     1621 ccgagcattt atgggccgca ctggattcta gtggcgctta cagtgggcac gtcgctcgtc
     1681 ggcattgtgc tatggggatg cgtggccggg gccatgctcc cattcctctt gaaaagagtt
     1741 ggtcttgatc cggccacatc ctccgcgccg tttgttgcta ctcttgtaga tgtaacgggg
     1801 ctcataatct atttcagtgt agcattcctg atcatgcgtg gaacgctgtt gtgaaacgtt
     1861 atgcgccatt tcttccggaa cgccagagga ctggattgca ttaaaaagag tttaccgtcc
     1921 taaaccgggt cgcataatca gggcaaggtg caatatgcaa aacgaaggat tgggttacct
     1981 gtggcaccag tttacctggg atgaaaacct acccggcctg attctgtttg gagtcctggc
     2041 agttgtgttt gctctgctga tcctgtactg gcggtatcga cagaaagagg ctcgtctgtg
     2101 ggaacgcctc ggaggactcg ctgttcggcg ccagctctcc gcagaagagc tagggctgct
     2161 tgaaaccttc tacgccgcgc ttccaagacc aaaccaggaa ctcgtactga aagacactaa
     2221 acgattctgg cagcttctgt ttcgattcag tcgtgcgcaa atgcggactc accctctgga
     2281 aatgattcga ctgatcaaca aactgtttcc agtggcaagg tttcaatgga acattaccgc
     2341 catagaagac gttcaaccgg gcgaaatggt agcggtgcaa cttgcaaaac aaaacctcat
     2401 cgcgcgagta gccctggtca atcgcgacca gaagagaatg gaactcgctt tcgcagataa
     2461 aaacttcgag gtggccgatt ccatttctgc ttctctctat ttctttcgtc cagggctcgg
     2521 agaaacgcgg atgagaggct cactgtatgc cgcatccagg ggccatgcgc tctttcaact
     2581 ggaaggagat aaaatagaag tcagcggaca gcagaaactg atggctcgtg cagaactctc
     2641 atttagcatg tcgcccatcg cggaggccag agatgaaggg aaatcagaaa cactcctcac
     2701 agggcgcaca gaatacgtct ctcctcgcgc actcatgatt caactcgacg cgccagcgct
     2761 ctacggcacc gcggaaaaga aaacagacgc ctggaagatg gat
//
LOCUS       DAORFF010000523         2789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_24961_length_2789_cov_3.457937, whole genome shotgun sequence.
ACCESSION   DAORFF010000523 DAORFF010000000
VERSION     DAORFF010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24961_length_2789_cov_3.457937"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PKK76_16780"
     CDS             <1..759
                     /locus_tag="PKK76_16780"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNL70187.1"
                     /translation="MKVICISNSKGGVGKTTTAVHLADSLAKDGRTLLIDIDVQANAS
                     SIFMEREVPAELTVYGALREKRPMGQLIHGTRNPGLDIVPATIQLAELESLLSGALDG
                     FFRLSESLRMQDLPYEFVIIDCPPNLGMVTVNAFVAATHVLVPLQTAKFSLDGLAALT
                     STIKTVQSRFNQGLKILGGVLTMYNPRTAIAHAMIEPIQEYIRVFDSKISRLVAVEEA
                     FLMKQTVFEYEPKSRVAKEYLALKKEVLRGIEKG"
     gene            740..1480
                     /locus_tag="PKK76_16785"
     CDS             740..1480
                     /locus_tag="PKK76_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70188.1"
                     /translation="MGSKKDKLGQAARRRLDETMGPPQGGNRSNMEDFVRDMRGEPAA
                     ASSEPAQSPESKRVSPRSDSSETGRKERPRMELKKSRRDSIYNISSDIRLEDYTDGGS
                     TSGGGIGGKARIIAGALLFVLLGLGIYYLAGWIFGPRYHLAVASQDITAENIEELEGR
                     EPSLSAGTDVHIRFQWPEEKLKATYIKVKVDRVDGGNKEEQAALGQKPPRTANYVYFK
                     GPLDPGVYHVEVMDESGSVMKDKEFKVR"
     gene            1480..2193
                     /locus_tag="PKK76_16790"
     CDS             1480..2193
                     /locus_tag="PKK76_16790"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70189.1"
                     /translation="MRVVLILFLAAAGCKDGLARLKEPPRPEAVPAGAERDRRTGIWG
                     YKTDKDWTLYNADGKKYAEGGLVLGLRQGEWKYYNVDGHTVLSRGKFLNDWREGVWDY
                     NDTRGVLYLRMVYTQEPKRELIALLTHDYGNENGPYERHYPDGSIEETGSFQAGYYEG
                     PITRFHPNGRLLLKGQYQKDLMVGRWLYYYPDGTILREENYVQGALDGLFRSYHPDGR
                     LYVETHYEKGAVVGPVVIR"
     gene            2198..>2789
                     /locus_tag="PKK76_16795"
     CDS             2198..>2789
                     /locus_tag="PKK76_16795"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNL70190.1"
                     /translation="MIRIRLPHGGRVWKGLLISIVIVLCILVGWNLFGSESGEGGGID
                     SIAEPGRRALLLNVKENISLVRRLEQNQREIHQILKDEPELFGKFKSDSEPPYDWSRP
                     SAMSMLRANADYAGIYSGFFREIPHRWPLRLDFLKLNSGYGSRGAVFGFLGQSEFHPG
                     FDLNAVAGTPVLAAADGVVKLALSSSYGYGNHVVILH"
BASE COUNT          718 a          654 c          763 g          654 t
ORIGIN      
        1 atgaaagtta tctgcatatc gaattcaaaa ggcggtgtcg ggaaaactac aactgcggtt
       61 catctagcag actctcttgc gaaagacgga cgcactcttc ttatagacat tgacgtgcag
      121 gcgaatgcaa gttctatctt tatggagaga gaagtgccag cagagctgac ggtgtacgga
      181 gcgctcagag aaaaacgccc catgggtcag ctgattcacg gcacgcgaaa tccgggactt
      241 gacatcgtcc ctgccacgat ccagcttgcg gaacttgaga gtctactttc aggcgctctg
      301 gacggtttct ttcgcctttc tgaatcgctt cgtatgcagg atcttccgta cgaattcgtc
      361 atcatagatt gtccgccaaa tctcggcatg gtcactgtaa atgcgtttgt ggctgccact
      421 catgtactcg ttcccctcca gactgcgaag ttttccctgg acggactggc cgcactcacc
      481 agcacaatca aaactgtgca gagtcgtttc aatcagggat tgaaaatact gggcggagtt
      541 ttgacaatgt acaatccaag gacggccatt gctcacgcga tgatcgaacc aattcaggaa
      601 tacattcgag tgtttgattc taagatttcg cggctcgtgg ctgtagaaga agcgttcttg
      661 atgaaacaga ctgtatttga atacgaacct aaaagcagag tggccaaaga gtacctggct
      721 ttgaagaagg aggtccttcg tgggatcgaa aaaggataag ctaggtcagg cagcgcggcg
      781 caggctcgat gaaacgatgg gccctcccca gggtggcaat cgttccaata tggaagattt
      841 tgtaagagat atgagagggg aacccgcagc cgcttccagt gaaccggcac aatcgccgga
      901 gtcgaaaagg gtttccccga gaagcgactc gtccgaaact ggacgtaagg agagaccgcg
      961 catggagctc aagaagtctc gcagggattc tatctataat atatcgagcg atatcagact
     1021 cgaagactac accgacggcg gcagcacatc cggcggcggc attggtggaa aggcaaggat
     1081 catcgcaggc gcgcttctct ttgtattgct tggacttgga atttattatc tcgcgggctg
     1141 gatctttggg cctcgctatc atcttgcagt tgcatctcaa gacatcaccg cggaaaacat
     1201 agaagaactc gaaggtcgtg aacccagtct ttctgcgggc acagacgttc acattcgctt
     1261 tcagtggcct gaggaaaaac tcaaagcaac ctatatcaaa gtcaaagtag atcgcgtaga
     1321 cggaggcaac aaagaagaac aggctgcgct tggtcaaaaa cctccgcgta cagcgaacta
     1381 tgtctacttc aaaggtccgc tcgatcctgg agtctatcat gtggaagtga tggatgaaag
     1441 cggcagcgtc atgaaagata aagaattcaa ggtgcgctga tgcgcgtcgt tctgattctg
     1501 tttctggctg cggccggatg caaggacggg ttggctcgcc tgaaagaacc tcccaggccg
     1561 gaagcggtcc ccgctggtgc agagcgtgat cgaagaacgg gcatctgggg atacaaaaca
     1621 gacaaagact ggactctcta caacgcggac ggaaagaaat acgcagaagg cggccttgtc
     1681 ctgggtcttc gccagggaga atggaagtat tacaatgtag acgggcacac agtgctgtct
     1741 cgcggcaagt tcctgaacga ctggagagaa ggtgtctggg attacaacga cactcgcggc
     1801 gttctgtatc ttcgcatggt atatactcag gagccaaaga gagaactcat cgctctgctc
     1861 actcatgatt atggaaacga aaacggtccg tatgaaaggc attatccgga cggaagcata
     1921 gaagagacag gctcttttca ggcgggatac tacgaaggcc ccatcactcg ttttcatcca
     1981 aacggtcgtc tacttttgaa gggccagtat caaaaagacc tcatggttgg ccgatggctg
     2041 tattactacc cggatggcac tatccttcga gaggaaaact atgttcaggg cgcgctcgac
     2101 gggctcttcc gatcctatca tccggacggc aggctctacg tggaaaccca ttacgagaaa
     2161 ggtgcagtcg tcggcccagt cgtcattcgg tagccgcgtg attcgcattc gccttccgca
     2221 tggtgggcga gtgtggaaag ggctattgat ttccatcgtc atagtattgt gtattctcgt
     2281 gggctggaat ctctttggtt ctgaaagcgg agaaggcggc ggcattgatt cgattgccga
     2341 gccaggcagg cgtgctcttc ttttaaatgt aaaagagaac atttcactcg tgcgaaggct
     2401 cgagcaaaat caaagagaga ttcatcagat tctcaaagac gagcctgaac tatttggaaa
     2461 attcaaaagc gattctgaac ctccttacga ttggagcaga cccagtgcga tgagcatgct
     2521 tcgtgcaaac gcagactatg cagggatcta ttccggattc tttagagaga ttcctcaccg
     2581 ctggcctctt cgcctggatt ttttaaaact gaattcagga tatggaagca gaggagctgt
     2641 ctttggtttc ctgggccaga gtgagtttca tcccggattc gatttgaatg ctgtggcggg
     2701 aaccccggtg cttgcggcag cggatggtgt agtgaagctt gctctttcat cgagctatgg
     2761 ttatggcaac catgtagtca ttttgcatt
//
LOCUS       DAORFF010000524         2780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_25115_length_2780_cov_3.398899, whole genome shotgun sequence.
ACCESSION   DAORFF010000524 DAORFF010000000
VERSION     DAORFF010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25115_length_2780_cov_3.398899"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKK76_16800"
     CDS             <1..226
                     /locus_tag="PKK76_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70191.1"
                     /translation="SLGLDFFIADLAQIKPGSNYWRRDVEHCASVIYLVALAIDQPQI
                     TARTASSPDSRWPGPLLCSLKEVGTWDLKQ"
     gene            complement(238..1455)
                     /locus_tag="PKK76_16805"
     CDS             complement(238..1455)
                     /locus_tag="PKK76_16805"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL70192.1"
                     /translation="MHEIVFRITTIIVAYIVLWTTLIELNIGIIYKENLRYLRLIIDS
                     CGLTIGILITGGASSPGIVTYAGTTGLASLYISKRFGVFSIATNVTLFNAMLFLVHEG
                     ILPRINVLAPDGLGSESTMAEIILANIMMILYSIILHLVIQPNYRNLHTERAELKARN
                     DAMHREITLARRIQEKLIPARSPDESIAFLYKPMDQVGGDFFDFIEHDGKIGIFVCDV
                     SGHGVPAALIASMMKTIVLQAGHRASDPATFMSYINETLQSRTAGNFVTAFYASFQKS
                     SRSIVYCNAGHCSPYVIDQTGVRELEGVKSVPLGLWSNDELRLRNRAFSNVEINLIAD
                     SRILFYTDGLTETRCENGDLMFEEGPLQENLVATRQQTSAAAVEDLHRALARFHGSNS
                     FEDDVCVICLDIK"
     gene            1760..>2780
                     /gene="lpxD"
                     /locus_tag="PKK76_16810"
     CDS             1760..>2780
                     /gene="lpxD"
                     /locus_tag="PKK76_16810"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNL70193.1"
                     /translation="MTLQELSQKLGMKCAGKADKEISGIKDLESPGDASPDHIYFVES
                     KKRLKKYSEKIAGVKVALTVPELAHNFEYAIVVDPPEMARLKFIELLGIFEKKSQPEK
                     GISPRASIHPTAKIGKDVSIMENVVVMKDCVIGDGCVLYPNVVVEERARVGAQSVLMA
                     GVVIGYNCSLGTNALIHANTVIGADGFGFYDKGGVRHKIPQIGNVIIGNHVEMGACCT
                     VDRATIESTLVGDYTKFDDHVHIGHNCRVGNYVFIAGATVLAGSVVLEDRVIIGGQSA
                     IAEHLRMRKGSVLLGMSALTADSQEGTIYFGIPALPAIVMHRIHALFPKLPELFRRLK
                     ESDAAE"
BASE COUNT          681 a          655 c          749 g          695 t
ORIGIN      
        1 gagtcttggc ctggatttct ttatcgcaga tcttgcacag atcaagcctg gttcgaatta
       61 ctggcgtcgc gacgtggagc actgtgcgtc tgtgatttat cttgtggccc tcgcaataga
      121 ccagccgcaa attactgcgc gtactgccag ttcgccggat agccgttggc cggggccgtt
      181 gttgtgctcc ttgaaagaag taggaacgtg ggacttgaag cagtgatgtc gctcgcttca
      241 cttaatatcc aggcagatca cgcaaacgtc gtcttcgaat gagttgctac catgaaaccg
      301 ggccaacgcg cggtggaggt cttctactgc ggccgcagaa gtctgttgcc tggttgccac
      361 caggttttcc tggagtggac cctcctcaaa catcagatct ccgttctcgc aacgtgtttc
      421 tgtgagtcca tcggtgtaaa agagtattct cgaatcagcg atcaggttga tctcaacatt
      481 tgagaaagct ctgtttcgca gacgaagctc gtcgttggac cagagtccca gcgggacgct
      541 ttttactccc tccagttcgc gtacgcctgt ctgatctatg acgtaaggcg aacaatgtcc
      601 cgcgttgcag tacacaatgc ttctgctcga tttctggaag ctcgcataaa aggcagtcac
      661 gaaattcccc gcggttcgac tctggagagt ctcgttgatg taggacatga atgttgcggg
      721 atcggacgct cggtgtccag cctgaaggac gatggtcttc atcatcgatg cgatcagtgc
      781 agctggaacc ccgtggccgg agacatcgca cacaaaaatt ccgatcttcc catcgtgttc
      841 tatgaaatca aagaaatctc cgcccacctg gtccattggc ttgtagagaa aggcgataga
      901 ctcgtctgga cttcgcgccg gaatcagttt ctcttgaatg cggcgggcca gagtaatctc
      961 tctgtgcatg gcgtcgtttc gcgctttcag ttccgcgcgt tccgtgtgta gattgcgata
     1021 attgggctgg atgaccaggt gaagtatgat ggaatacaga atcatcatga tattcgcaag
     1081 tatgatttcc gccatcgtag attccgatcc cagcccatcc ggggccagaa cgttgatgcg
     1141 tggcagtatg ccctcgtgaa ccagaaacag catggcgtta aagagcgtga cattggttgc
     1201 tatggaaaag acaccgaatc gctttgaaat gtagagcgac gcaagacccg ttgtccctgc
     1261 gtatgtcacg atgcccggag aactggcccc tcccgtgatg aggattccaa tggtcaggcc
     1321 gcaggaatca atgatgaggc gaaggtatct taaattttct ttataaataa ttccaatgtt
     1381 tagctcgatc agcgttgtcc acaaaacaat gtaggccacg atgattgtag taatccgaaa
     1441 aacaatctcg tgcattccga tctgatggaa ccatgtcagc gccccgagtc cgatcagcag
     1501 ccttgggata ttgttgcttc tgaatctttt tctgcgctgg tgccggtctt cgttcacgat
     1561 ggatgcatcc tgtgccgatt tgccggcgat tgcaaccaaa acaagcgtgc ggagaattag
     1621 gaaacatccg tccgttctgc cttgacgttt tgatcgtttc cgggcacggg tgcgaaacaa
     1681 gactgcgcat ggattggaca aatccatttg attttaacgc tgttcaatac ggtgtgcgcg
     1741 tcacgatgtc ggaggaccta tgactctaca ggaattgtct caaaagctgg gcatgaaatg
     1801 cgccgggaag gcggacaagg aaatttcagg aatcaaagac ctggaaagcc cgggggatgc
     1861 atctccagat cacatttact ttgttgagtc aaaaaagagg ctgaagaaat attcggagaa
     1921 aattgcgggt gtaaaagttg ctctcactgt gccggagcta gcgcataact tcgagtatgc
     1981 gattgttgta gatcctccag aaatggcgcg tctgaagttc atcgagcttc tgggcatctt
     2041 cgaaaaaaag agccagccag aaaagggaat ttctccgcgc gcgtccattc atccaaccgc
     2101 aaagattggc aaagacgtat cgattatgga aaacgttgta gtcatgaagg attgcgtgat
     2161 cggagacggt tgtgttctct atccaaacgt tgttgtagaa gaacgcgcca gagttggtgc
     2221 tcagtcggtt ttgatggccg gagtggtgat tggatacaac tgcagcctgg gaacaaacgc
     2281 actcattcac gcgaacaccg tcattggcgc agacggattt ggattctacg ataaaggcgg
     2341 agtgcgacac aagattcctc agattggaaa cgtaatcatt ggaaatcatg tagaaatggg
     2401 agcttgctgc accgtggatc gcgctacgat cgaaagcact ctcgtaggag actacacgaa
     2461 gttcgacgat catgtgcaca tcggccacaa ttgcagagtt ggaaattacg tctttattgc
     2521 aggggcgacg gttcttgccg gttcagtggt tctggaagac agggtgatca taggagggca
     2581 gtctgcgatt gcagaacatc taagaatgcg aaagggatct gttctactgg gtatgtctgc
     2641 actcacagca gattcgcagg agggaaccat ttattttggt attcctgctc ttcctgccat
     2701 cgtcatgcac agaatccacg cactgtttcc aaaactgccg gaacttttca ggcggttgaa
     2761 ggaatcagat gcagcggagc
//
LOCUS       DAORFF010000525         2780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_25116_length_2780_cov_3.289174, whole genome shotgun sequence.
ACCESSION   DAORFF010000525 DAORFF010000000
VERSION     DAORFF010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25116_length_2780_cov_3.289174"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PKK76_16815"
     CDS             complement(<1..785)
                     /locus_tag="PKK76_16815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNL70194.1"
                     /translation="MAPDYITMAIPVFLFLIAVEIIVSRLKKGGYYDYHDSVNDLSAG
                     ILSQVVGVFGKIITVGLYVYVYQNWRLLEISEKSVIAWVACFFGVDFFYYWFHRIAHE
                     SAVFWGSHLPHHQSEEYNLTVALRQGAFQSFFSIWFYVPLALVGIPPLIFLVHSQFNT
                     IYQFWIHTRAVGKLGFLEIFMNTPSHHRVHHGKNPIYIDKNYAGTLIIWDKMFGTFQE
                     ETEEVHYGTIKPLASWNPIWAQVHYFLELFQKSPVVVEVLFIIV"
     gene            923..1780
                     /locus_tag="PKK76_16820"
     CDS             923..1780
                     /locus_tag="PKK76_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNL70195.1"
                     /translation="MKTVVIDGVTVQLASPDLIDQNWVGNMDVLNQLMACWVLVHPSD
                     LPLNPRLIGRPGVGKTTLACAAARVLDKPTYIFQATMDTRPEDLLITPVISESGKIKY
                     VASAIVTAMLEGGIAILDEGNRMGEKSWASLAPLLDHRRYIESIVAGIKIKASPEFRF
                     CATMNEDASTYEIPEYIQSRLQPQIYIDFADADEELEILRANLPFAPREILNYVVSFL
                     QKAHSADEPFSVRDGINITRLVLKLGVSREFIQREGESFTVDEKRLRELLHDAVMGIL
                     GDDALRYLA"
     gene            1777..>2780
                     /locus_tag="PKK76_16825"
     CDS             1777..>2780
                     /locus_tag="PKK76_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70196.1"
                     /translation="MTESHHDPFEETVPLLPGVRAFPIVHGSVEYTVILHQILRAQKP
                     GAIAVELPADLSGALDQVLPSADQIPVISFDDAGAQSVHFILEPLEPIVEAVRSAKDR
                     GIHTHLIDLPCLSASPADAEFFSPASYGFSPEQFPDTFSLRVMSARTVYDLYRKNQLP
                     VRYESLDPSSLRGDFIRELFMAEKIRKLAPLYSTPRTGRIDGCLLVVCGIKHLPAIEH
                     LVALPEHEFERFRGILGEIQRYRGQEEPEDDEEPLEALMKGSEARNSAPDVEISVLSR
                     ESPEVLSQPGYYNTTWNLVRRSERTVKAFNRIVLQRAVYRESVSRFEKESGEALSPRI
                     "
BASE COUNT          724 a          661 c          695 g          700 t
ORIGIN      
        1 actatgatga acagtacttc aactaccacc ggcgattttt gaaaaagttc gaggaagtaa
       61 tgaacctgtg cccagattgg attccagctg gccagaggtt tgatggtccc gtagtgcact
      121 tcttctgttt cttcttgaaa cgttccgaac atcttgtccc agatgatcaa cgttccggcg
      181 tagttcttgt ctatgtagat tggatttttg ccgtgatgca ctctatgatg agaaggagtg
      241 ttcatgaaga tttccaggaa accaagtttg ccaactgcgc gagtgtgaat ccagaactga
      301 tagattgtgt tgaactggga atgaaccagg aagatcagag gtggaattcc gaccagtgca
      361 agcggaacat aaaaccaaat ggagaagaaa ctttgaaaag cgccctggcg tagagccaca
      421 gtcaggttgt attcttcgct ttgatggtgg ggaagatgcg atccccagaa gactgcagat
      481 tcatgagcaa tgcgatggaa ccagtagtag aaaaaatcca cgccaaagaa gcatgcaacc
      541 caggcaatca ctgacttttc tgagatttca agaagccgcc agttctgata cacgtaaacg
      601 tagagaccga ccgtaatgat tttgccgaat accccgacga cttgagaaag aattccagcg
      661 ctcaagtcgt ttacagagtc gtggtagtca tagtatccac ctttcttcag gcgtgagacg
      721 atgatctcca ccgcgatgag aaacaagaat accggaattg ccattgtgat gtagtcaggc
      781 gccatgcgaa aaatcccctt gttatgtctg gtttaatgat aaaaagtaag gccgcaagcg
      841 cgaaaattta gatttttgaa tctcaaatga ttgcgcgaat gattcgcctc agttcagctg
      901 acaaagccgc aaacgcataa atatgaaaac tgtagtaatc gatggtgtaa ctgttcagct
      961 tgcaagtcca gatttgatcg accagaactg ggtcggaaac atggacgttc tcaatcaatt
     1021 gatggcgtgc tgggttctcg ttcatccaag tgacctgcct ctcaatccaa ggttgattgg
     1081 ccggccggga gtaggaaaaa ctactctggc ctgtgcagcc gcgcgtgtcc tggataaacc
     1141 aacctatatc tttcaggcta ccatggatac caggccggaa gatctgctca tcactccggt
     1201 catttcagaa tcaggaaaaa tcaaatacgt ggcttccgcc attgtcaccg ccatgctaga
     1261 aggtggaatt gctatactcg atgaaggcaa tagaatgggg gaaaagtcct gggcgtccct
     1321 ggcacctttg ctcgaccaca gacgttacat cgagtccatc gttgctggca tcaagatcaa
     1381 ggcaagtcct gagtttagat tctgcgctac aatgaatgaa gatgcttcca cttacgaaat
     1441 cccggaatac atccagagca gactccagcc gcagatctac atagactttg cagacgcgga
     1501 tgaagagcta gagattctgc gcgcgaattt gccttttgca ccgcgcgaaa tcttgaacta
     1561 cgttgttagt tttctacaaa aagcccacag cgcagatgag cctttttctg tgcgcgatgg
     1621 aatcaacatc actcgcctgg ttctcaagct cggcgtttcg cgtgagttca ttcagagaga
     1681 aggcgaatct ttcacggtag atgaaaaacg tctgcgtgaa cttttgcatg atgcagtcat
     1741 gggcatcctg ggcgatgatg ctcttcgcta tctggcatga cggaatcgca tcacgatcct
     1801 tttgaagaaa cagtcccgct tttgccggga gttcgcgcat ttcccattgt gcatggatcg
     1861 gtcgagtata cggtaatcct gcatcagatt ttgcgcgcgc aaaaaccagg cgctattgcc
     1921 gttgagttgc ccgcggatct gtccggagcc ctggatcaag tattgccgag cgcagatcaa
     1981 attccagtga tctcttttga cgacgctggc gcgcaatcag tgcacttcat tctggaacca
     2041 cttgagccaa tcgtggaagc tgtgcgaagt gcgaaggata ggggaattca tactcatctg
     2101 atcgatcttc cttgtctgtc cgcaagcccc gcggacgcag aattcttttc tcctgcgagc
     2161 tacggttttt ctcccgaaca gtttccggat acgttttcgc ttcgggtgat gagtgctcgc
     2221 acagtatatg atctttatag aaaaaatcag ctcccggtgc gatacgagtc tctcgatcct
     2281 tcttctttgc gaggagactt tattcgcgag cttttcatgg cggagaaaat tcgcaaactc
     2341 gctccacttt attctactcc acgcaccgga cgcatagatg gttgcctcct ggttgtgtgt
     2401 ggaatcaagc atctgcctgc cattgagcac ctcgtcgccc tgccggagca tgagttcgaa
     2461 cgcttccgcg gaattcttgg agagattcaa agataccgcg gccaggaaga acccgaagac
     2521 gatgaagaac ctctcgaagc attgatgaaa ggatcggaag cgcgtaactc tgctcccgat
     2581 gttgagatca gcgtgctttc gcgcgaatcg cccgaagttc tttctcagcc tggttactac
     2641 aatactacgt ggaatctggt tcgcagatca gagaggacgg tcaaggcgtt caatcgaatt
     2701 gtactccagc gtgccgtgta tcgtgagtct gtaagcagat ttgaaaagga aagtggagag
     2761 gcactctccc caagaattga
//
LOCUS       DAORFF010000526         2775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_25199_length_2775_cov_2.685662, whole genome shotgun sequence.
ACCESSION   DAORFF010000526 DAORFF010000000
VERSION     DAORFF010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25199_length_2775_cov_2.685662"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="PKK76_16830"
     CDS             <1..1416
                     /locus_tag="PKK76_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70197.1"
                     /translation="MRTARPTQSYPVERDLPGQRGQNVCSDLLDRTGDFNTPLRIAIR
                     SAADVPFQSFFKFVGDNSDPAAGAFLRNSSSVLKYAKMTGLMDKVMGVKYDFALNPIP
                     AEQHYNVGSQTNVRASFKASVTLNQRLTNRDNAALDCARLLGITVPDTTEAMRRAAGG
                     FRVYWFGVAGFPTYAEIMDGESRFNYHAPLGSDLTLSGNTAFATMIVRPATESQQAHN
                     AGLQKRESVSVVAQLDKSDANLIQNLINAVRSGVGPQLAIDPAVNLMKKLFGQKREAT
                     LTVTHHQPFRLAGTIRGKFAFPRQGAGPAHELKGSEELEVFFRENGTVAASYKLDWGY
                     ADMRCKNRTSNYIWTVKDSGKQNLAISDFQVQVDEGGNYTIRFPLNEFFQDIGPWHPL
                     DPMCKPGPLDYANLRGFGSPDQLITLRGTIPEGSRTMSGNLTQTDAEFLNIGFSNGSS
                     SVRAPTGARTLQWNLRITDAP"
     gene            1466..>2775
                     /locus_tag="PKK76_16835"
     CDS             1466..>2775
                     /locus_tag="PKK76_16835"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNL70198.1"
                     /translation="MLLFSYYATSYLSATIFVLALVIYLAFLPAKTRHTRLLLIYFIV
                     TATLSVGFLLRSSLFVASVARPASYLIALYTCFSNAALLAFVYSFPRNSYDREARIAV
                     GVFVAMGLVGYLYYVSTTWSLPVRYNDVTLFHEFQTPGSARPMGFWHTASFLWILFVA
                     LRKLRRGSHGPSGRLLWERQAARAFTAAIATQFLFTFSYLLYTAQIISFEKFQFVLSG
                     ANAIQLFLYSVIYLNYSPQPSTFMAKLVGISLVTVLLILALVYRVTFDHHREAFESEL
                     MRRIELVRSDSSLAAGFGFKNIRTHEGTEDSCGTWPQVNRTFFASDVNDISTYTWQFC
                     VRRGGSSLVLDFPYVLFRQSMHEHMKTLVKIMIGAALLFLVIFPFFFYYGVAFPLRLI
                     LAAVRDVNAGRYQTRVDVLTADELGIVARSFNRMVRSIREGRARL"
BASE COUNT          604 a          693 c          704 g          774 t
ORIGIN      
        1 atgcgcactg cgagacccac gcaatcgtat cccgtggaac gcgatttgcc gggtcagcga
       61 ggtcaaaacg tttgctctga tctcctcgat cgcacgggag acttcaatac tccgttgcga
      121 atcgcgattc gatctgcggc tgacgttcca tttcaaagtt ttttcaagtt tgtaggggat
      181 aacagcgacc cggctgcagg tgcatttctt cgcaatagtt cttcggtgtt gaagtacgca
      241 aagatgacgg gtctaatgga caaagtaatg ggggtgaagt acgattttgc tttgaatccc
      301 attccggcag agcaacacta caatgtagga tctcaaacca atgtccgtgc gtctttcaaa
      361 gcttctgtta cacttaatca gagattaacc aaccgtgaca atgcggccct ggattgtgcc
      421 cgtcttctcg ggattactgt tcctgatact acggaagcga tgcgtcgcgc cgccggcggt
      481 tttcgggttt actggttcgg agtagcgggc tttcctacgt atgcggaaat tatggatgga
      541 gaaagtagat ttaactatca cgctccgctc ggaagcgatc taacgttaag cggcaataca
      601 gcgtttgcca caatgatcgt tcggcctgca actgaaagcc agcaggccca caacgcaggg
      661 ctgcaaaagc gagagagcgt ttctgtcgtg gcgcaattgg ataagagcga tgcaaatctc
      721 attcagaatc tcatcaatgc ggttcgttcc ggggttggtc cgcaactggc catcgacccc
      781 gccgtcaatc tcatgaaaaa actgtttggt cagaagagag aggcaactct taccgtgaca
      841 caccatcaac cgtttcgttt agcgggaacg attcgcggga agttcgcttt tccgcgccag
      901 ggtgcaggcc cggcgcatga gttgaagggc agcgaagagc tcgaggtctt cttccgcgaa
      961 aacggtactg tggcagcgag ttacaagctt gactggggct acgcagacat gaggtgcaag
     1021 aaccgcacgt ccaactatat ctggacggtg aaggattcag gaaaacagaa tctggctatt
     1081 tcggactttc aggtccaggt agatgaaggc ggaaattaca caatccgctt tcctctcaat
     1141 gagttctttc aagacattgg accctggcat ccgctcgatc ctatgtgcaa acccggtcca
     1201 ctggattatg cgaatttgcg cggatttggc tccccggatc agctcatcac attgagagga
     1261 acaattccag aaggatcgcg tacaatgagc ggcaatctta cacagacaga tgcggagttt
     1321 ctcaatattg gattttccaa cgggagtagt tctgtgcgag ctcccactgg agcgcgaact
     1381 ctgcagtgga atctgaggat cacggacgcg ccctgaaagg caatcggatt gtattctggc
     1441 tgctctctga tgttctggct ttgagatgct cctcttttcc tattatgcga cgagttatct
     1501 gtctgccacg atctttgtcc tcgcgctcgt gatctatctc gcattccttc ctgcgaaaac
     1561 acgtcacaca aggttattgc ttatatattt tatcgtcacg gcaactttgt ccgtgggatt
     1621 cctgcttcgc agttctcttt ttgtcgccag cgtcgctcgt cctgcgtcct atctcatcgc
     1681 gctttatacg tgtttttcga atgcagcgct tcttgcattt gtgtacagtt ttccgcgcaa
     1741 cagttatgac cgggaagcgc gcatcgcagt gggtgtgttt gtcgccatgg gcctggtcgg
     1801 gtatctctac tacgtttcga ctacgtggag cttgcccgtc cgctacaatg acgtcactct
     1861 ctttcatgaa tttcaaactc cggggtctgc cagacccatg ggtttctggc acaccgcatc
     1921 ctttctgtgg attctatttg ttgctcttcg caaattgcga cgtgggagtc acggtccttc
     1981 cggccgtctg ctctgggaaa gacaggctgc acgtgctttt actgcggcaa ttgcaactca
     2041 gttcctgttt acattttctt atcttctgta taccgcgcaa atcatcagct ttgaaaagtt
     2101 tcaattcgtg ctcagcggtg caaatgcgat ccagcttttt ctttacagcg taatttatct
     2161 caactattca cctcagcctt ccactttcat ggcaaagctg gtaggcattt ctcttgtgac
     2221 agtcctattg attctggccc tcgtatatcg cgtaacgttc gatcatcata gagaagcgtt
     2281 tgaatcggaa cttatgcgca gaattgaact tgtgcgcagc gattcgagtc ttgcagcggg
     2341 gttcggtttc aagaatattc gcacgcacga gggaactgaa gactcatgcg gcacgtggcc
     2401 gcaggtaaac agaacttttt ttgcaagtga cgtgaatgat atttccacat acacctggca
     2461 gttctgcgtt cgcaggggcg gaagttctct ggttctcgat tttccatacg ttcttttccg
     2521 ccagtccatg cacgagcaca tgaagacgct agtgaagatc atgatcggcg cggcattgct
     2581 attccttgtt atttttccct ttttctttta ctacggagtt gcatttccac tgcgtttgat
     2641 tttggccgcc gtgcgggatg tcaatgcggg ccgctatcaa actcgcgtgg atgttttgac
     2701 ggcagatgaa ttgggaattg ttgctcgttc ttttaaccgg atggttcgat ctattcgcga
     2761 aggccgggcc cgact
//
LOCUS       DAORFF010000527         2772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_25248_length_2772_cov_3.300331, whole genome shotgun sequence.
ACCESSION   DAORFF010000527 DAORFF010000000
VERSION     DAORFF010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25248_length_2772_cov_3.300331"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..924)
                     /gene="larE"
                     /locus_tag="PKK76_16840"
     CDS             complement(73..924)
                     /gene="larE"
                     /locus_tag="PKK76_16840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNL70199.1"
                     /translation="MLRVDRNILWIMPVSSELMARRERLIHLLSPHTVPGLIVAFSGG
                     VDSACLLWAAREAAKKSAGRVLAITAVSASVPDLDLRDASEFAEKLGVEHRILRSRET
                     ENQEYLKNDSNRCFHCKTELFRIAREEADAEGIQHIAYGYTISDRGDVRPGHRAARMA
                     GVLAPLEEAELTKEDIRAILREEGFSLSEKPAAPCLSSRIPTGLPVTIKRLEDVQQVE
                     TFLRDHGVQIVRARIHDEKTIRIETEDASAAKVLELRHDLERLSRSLGYRWITLDLGG
                     YRTGGST"
     gene            978..1544
                     /locus_tag="PKK76_16845"
     CDS             978..1544
                     /locus_tag="PKK76_16845"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNL70200.1"
                     /translation="MKIYTKQGDAGWTRLANGEKTRKSDVRVDLYGTADELNSHIGLA
                     LSQPGVPEETRAQLLDLQHLLFELGSELAGYYKNKEGSIIREEDTGKLEQWMDQCTAV
                     LPPLRAFILPGGEAAASALHICRTVCRRLERAMTAALDENSGERPVVYPEAYKFINRL
                     SDYLFIAARYVNFRAGKSDIEWQTRETK"
     gene            complement(1718..>2772)
                     /locus_tag="PKK76_16850"
     CDS             complement(1718..>2772)
                     /locus_tag="PKK76_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70201.1"
                     /translation="YWEPGIGLVLENRILSDQMTSNYAVFLALTLGLHLLAPIVWICV
                     EQVNPELEAGDMASRLSIGILWGIGANLIVMLLQGFVSPLNTGITDRAIEAGRRAGLF
                     SDSGSAGAAFLAFVLPASFAFASLFHARIKKNKRWLASLLFFWAIAIFTASFFGRVIV
                     LASVGALVAWLFLIPLNRRVRLIAGFAILFSFVCFGLFLIWSGPVAFLSRIDPVRAQL
                     LETGIVLIRQSPWLGHGFNSFVPALMELRKSDPTILIENPAGLIPGILNDAGIVGLCI
                     LVAMLVYYVLGLRLLFLRKGADENAAEATVVVILSTLSMFLVFTVGYHILAAEVAAFV
                     FFPLLVLPDSFRRTKS"
BASE COUNT          794 a          742 c          665 g          571 t
ORIGIN      
        1 gacgaatgac tccgtccaca ttctcttcgc cggaaatcaa acgctcgagc agcttgcgaa
       61 cgtcattcaa attcaagtcg aaccgcccgt cctgtagccg cccaggtcca gcgttatcca
      121 ccgatagccg agagatcggg atagtctctc aagatcatgt cgcaattcga gtacttttgc
      181 cgcggacgca tcctctgtct cgatgcgaat cgtcttttca tcgtgaattc gcgctcggac
      241 aatctgcact ccgtgatctc gcaaaaatgt ttccacttgc tgcacgtctt ccagtctctt
      301 gattgtcacg ggaagaccgg tcggaatgcg agaggaaagg cagggcgcgg ccggtttttc
      361 agaaagtgaa aacccttctt cgcgcagaat ggcccggata tcttccttgg tcagttccgc
      421 ttcctcaaga ggagcaagaa cacccgccat tctggccgct cgatggcctg gacgaacgtc
      481 gccacgatca gagatcgtat aaccatacgc tatgtgctgg attccttccg catctgcttc
      541 ttctcgtgca atccgaaaca gttccgtctt gcaatgaaag caacgattcg aatcattctt
      601 taaatattcc tggttctccg tctcgcggct ccgcagaatt ctgtgctcca cgccgagctt
      661 ctctgcaaac tcagacgcat ctcgcaaatc cagatcggga acgcttgcgc tgaccgcagt
      721 aatggcaaga acgcggccgg cggacttttt ggcagcttca cgagccgccc agagcaggca
      781 cgcactgtcc actccacccg aaaaagccac aatcagaccg ggcacagtgt gcggagacaa
      841 aagatggatc agtctctctc tcctggccat caattccgaa gaaactggca tgatccatag
      901 aatgttgcga tcgacacgaa gcaaccaatg tgcaaaaaat attgaacgca ggtgcactcg
      961 ctcgattgtg gggtgctatg aaaatctaca cgaaacaggg cgatgctggt tggaccagac
     1021 ttgcaaatgg agaaaaaacc agaaaaagcg atgttcgtgt cgatctatac ggcacggcag
     1081 atgaactgaa ctctcacata ggcctggccc tttctcagcc aggcgtcccg gaagagacgc
     1141 gcgctcagct gctggatctg cagcatcttc ttttcgaact tgggtcagag ctcgccggtt
     1201 attacaagaa caaagaaggt tccatcatac gcgaagagga tacaggcaag ctggaacaat
     1261 ggatggatca gtgcactgca gtgctacctc cgttgcgtgc attcatactg ccggggggag
     1321 aagcggccgc gtccgcattg catatctgca gaactgtgtg caggagactg gagcgagcca
     1381 tgacagctgc acttgatgaa aattcaggtg aaagacctgt cgtatatccg gaggcgtaca
     1441 aattcatcaa tcgactgtcc gactatcttt tcatcgcagc aaggtacgta aattttcgcg
     1501 ctggcaaaag tgatatcgaa tggcaaacgc gagaaacaaa atgattttta attgccttca
     1561 ccgcggctca aagtaactca agcatgttag cagttcgatt gaattcaagg agcattcaaa
     1621 tgggcgaaaa aaacaaaggc aaccgcgaaa agaaaaaacc aaaacaagac aaaaaaccta
     1681 agtaatgatg cagcggccgt cgtctttgat gcgccgctca gcttttggtt cgcctgaatg
     1741 aatcgggcag aaccagaagc gggaaaaaca caaacgctgc cacctccgcc gcgagaatgt
     1801 ggtaccccac agtaaacaca agaaacatag agagagtgga aaggatcacc acaacggtgg
     1861 cttcagcagc attttcatct gctccttttc tcaagaacag caagcgcaat cctagcacat
     1921 agtatacaag cattgcaacc agaatgcaaa gacctacaat tccggcatca ttgagaattc
     1981 ctgggatcaa tcctgccggg ttttctatga gaatggtggg atcgcttttt cgcagctcca
     2041 ttaaagcggg aacgaagcta ttgaacccat gtccaagcca gggactttgc cgaatcaaaa
     2101 cgattcctgt ttccaaaagc tgagcgcgaa cgggatctat gcgcgacaaa aatgcaaccg
     2161 gcccactcca gatcaggaaa agtccaaaac agacaaaaga aaacaaaata gcgaagcctg
     2221 caatgaggcg aacgcgcctg ttcaaaggaa tcaagaagag ccacgcaacc agggcgccca
     2281 cgctcgctag cacaatcacg cgcccgaaaa atgacgctgt gaaaatcgca atcgcccaga
     2341 agaagagcaa agaagccaac catctcttat ttttcttgat cctggcgtga aacaacgaag
     2401 cgaaagcgaa actcgcaggc aaaacgaaag caagaaacgc ggcaccggca gatcctgaat
     2461 cagaaaacaa tcctgccctc cttcccgctt cgattgcgcg atccgtgatt ccagtgttca
     2521 gtggcgaaac aaatccctgg agtaacatga cgatgagatt cgccccaatt ccccagagga
     2581 ttccaatcga gagccgggaa gccatgtcgc ccgcttctaa ctctggattc acttgctcga
     2641 cacaaatcca gacgataggt gcgagcaaat gcaatcccag agtcaaagcc agaaagacgg
     2701 catagttgga tgtcatttgg tccgacagga ttctattttc caggacaagt ccaatccccg
     2761 gctcccagta gt
//
LOCUS       DAORFF010000528         2763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_25409_length_2763_cov_3.997046, whole genome shotgun sequence.
ACCESSION   DAORFF010000528 DAORFF010000000
VERSION     DAORFF010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25409_length_2763_cov_3.997046"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1122)
                     /locus_tag="PKK76_16855"
     CDS             complement(43..1122)
                     /locus_tag="PKK76_16855"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNL70202.1"
                     /translation="MPIARNYNGGSIVYFAGDKGDEVYVLQAGRVILISTALDTGEEI
                     REDVQIGEFFGVKSSLGKFPREETAQVIGKTTCIVFKLHEFEQLVMKNTHLILKMLRV
                     FSKQLRNIHRQIRDILKAGAAKDPSFELMSVAESFYRSGNVDHAVYALEKYLEVYADG
                     DYTDRAHDLLSQARKGLTYPHGYPPLQIADQKHKVDASFVTRSMASADSLGDDPFALE
                     EGAPAARPAEKEPGAADILMDGLTLMSQNKADAALAKFNECLAIKKLHTADDQEAAER
                     AQYEKGRAELKLGKVDDASNSFSIYLKKFPTGEFMKHAIFQLGVAAEAKGNKERARTL
                     YHKVATMPPPDDATRDARKRLEKLG"
     gene            complement(1124..2329)
                     /locus_tag="PKK76_16860"
     CDS             complement(1124..2329)
                     /locus_tag="PKK76_16860"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNL70203.1"
                     /translation="MLWQLTNGTLLLPDGPRKLSGLLVSGGRIEEVLAPGDDQPDILT
                     LNLHGALVIPGLINSHDTLMATYLPTKGENWPHLNWLSFDIETKRSALFRERMLLDVE
                     TLYRMGSLKNLESGVTAVVDHVPDFVRNPFLSSMSVEILPDFGIAHSVCSYSLGWGQG
                     LEAEHKRAVENDLPFILHIAEGFDPESELSLRVLDRAGCLSEHTVLVHGLSLSNDDLD
                     RIAEAGAHFVWCPSANLFAYNRTVKISEFLTRGIPVCLGTDAAVTGSTHMLSEIQTAL
                     KVYKDQTGEDLPDSDLFAMLTTNPARAFRWAGRGSLKPDSLADFVVLSCPSLREAKPE
                     DIYLVVRGGQPVYGSEDLEPLFISAAVHVERVDIGGTRKIVQKGVKDLRETVRQATGR
                     ESFPFLSGW"
     gene            2469..2741
                     /gene="rpsT"
                     /locus_tag="PKK76_16865"
     CDS             2469..2741
                     /gene="rpsT"
                     /locus_tag="PKK76_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNL70204.1"
                     /translation="MANLRASKKDIRKTRKRNEQNSQSRARLRTLDKKIRTLVAEGKT
                     EDAATAFKSYTQFLARAGQKNLIHHRQADRRKSRMAALLNRQKKSA"
BASE COUNT          606 a          735 c          736 g          686 t
ORIGIN      
        1 acaccttgcc aaacttatcg aataaggatt ctggaagaag catcagccca gtttctccag
       61 gcgctttctt gcgtcgcgcg tcgcatcatc cggaggaggc atggtggcca ctttgtgata
      121 aagagtgcgc gctctttctt tgttgccctt tgcttctgcc gcaactccaa gctggaagat
      181 cgcgtgtttc atgaattcgc cggttggaaa cttctttaaa taaatggaga agctgttgga
      241 tgcatcgtct actttgccga gctttagttc cgcgcgtccc ttttcatact gtgctctttc
      301 tgctgcttcc tgatcgtccg cagtgtgaag cttcttgata gcgaggcatt cattgaattt
      361 ggcaagagcc gcgtctgcct tattctgact catcaatgtg agtccatcca tgaggatgtc
      421 tgcagcgccc ggttcttttt ccgcaggtcg tgcggccggc gctccttctt cgagcgcgaa
      481 aggatcatct ccgaggctat ctgcggacgc catgctgcgc gtgacaaagc ttgcatccac
      541 tttgtgtttt tgatccgcaa tctggagtgg aggataaccg tgtggatacg tcaggccctt
      601 gcgggcctgc gacaatagat cgtgcgcgcg gtccgtgtaa tctccgtccg cgtagacctc
      661 taagtatttt tcaagtgcat agactgcatg atccacattc ccggatcgat agaaactttc
      721 tgctacactc atgagttcga acgacggatc tttcgcggca cccgctttga gtatgtcccg
      781 gatttgtctg tggatgttgc gaagctgctt ggaaaatacg cgcagcattt tcaaaatgag
      841 gtgcgtattc ttcatcacaa gctgctcgaa ctcgtgcagc ttgaaaacaa tgcatgtggt
      901 cttgccaatt acttgcgcag tctcttcacg cgggaactta cccagggagg attttacgcc
      961 gaagaactca ccgatctgta cgtcttcgcg gatttcttcc cctgtatcca gggctgtgct
     1021 gatgagtatg acacggccgg cctgaagtac gtagacttcg tcgcctttgt ctccggcgaa
     1081 gtagacgatg gagccgccgt tgtaatttcg agctattggc atatcaccag ccggagagga
     1141 aagggaaact ttctcgtcct gtagcctgcc tgactgtttc ccgcaaatcc ttcacccctt
     1201 tttggacaat ttttcgagtg ccgccaatgt ccacacgttc cacatgcacc gcagcagaaa
     1261 taaacagcgg ttccaggtcc tcgcttccgt ataccggctg gcctccgcgg accaccaggt
     1321 agatatcttc tggctttgct tctcgcaaag aaggacagga cagcaccaca aagtcagcca
     1381 gagagtccgg cttgaggctt cctctgcccg cccagcgaaa ggccctggcg gggttggtcg
     1441 tgagcatggc gaacagatct gaatcgggca gatcttcccc ggtctggtct ttgtagactt
     1501 tcagcgcagt ttggatttca gaaagcatgt gggtcgaacc agttacagcc gcatccgtgc
     1561 ccaggcatac cgggatgcct ctggtcagga attctgagat tttgacggtt cgattgtatg
     1621 caaaaaggtt cgcggacgga caccacacaa aatgtgcgcc tgcctctgcg atgcgatcca
     1681 ggtcatcgtt ggacaaagaa aggccgtgca ccaggaccgt gtgttcgctc aggcagccag
     1741 ctcgatcgag gacgcgcagg gacaattcgc tttccgggtc gaatccctct gcgatgtgca
     1801 ggatgaaggg caggtcgttt tctactgctc gcttgtgttc tgcctcgagc ccttgacccc
     1861 agccaagaga gtacgagcaa acgctgtgag cgattccaaa atcaggtagg atctccacgc
     1921 tcatgctgct caggaatgga ttgcgaacaa agtctggcac atggtcgact acggccgtca
     1981 ccccggactc gaggttcttc aggctcccca ttctatacag ggtctccacg tccaggagca
     2041 tcctttcgcg gaataaagcg gatcgctttg tctctatatc aaaggagagc cagtttaagt
     2101 gtggccagtt ttctcccttc gtcggtagat aggtggccat cagggtgtcg tggctgttga
     2161 tgagccctgg aattaccaga gctccgtgaa ggttgagggt gagtatgtcc ggctggtcat
     2221 cgcctggagc gagcacctct tcgattcttc ctccggacac aagcaggccg gagagctttc
     2281 ggggcccatc aggaagaagc agagttccgt tcgtcagttg ccagagcatg gtccgttcca
     2341 tctattgtcg gtgcaaggcg atgccattct tcaggccggc cggcgaatcg tggcaaagaa
     2401 gaaggaggat aaaaaactgc tggaccgtct ccccctgaac aattttctgg ccgttcaggt
     2461 gtaagtaatt ggcaaactta agagcatcca agaaagacat tcgtaaaact cgtaaacgta
     2521 acgagcaaaa ctctcaatcc cgcgctcgtc tgcggacact ggataagaag attcgcactc
     2581 tggtggctga aggcaagacg gaagatgctg caactgcgtt caaatcttac acccagttcc
     2641 tggctcgcgc aggtcaaaag aatttgatcc accatcgcca ggcagatcgc agaaagagcc
     2701 gcatggcggc tctactcaac aggcagaaga agtcagcctg atagttttcc ttttgggcga
     2761 tta
//
LOCUS       DAORFF010000529         2755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_25551_length_2755_cov_3.727037, whole genome shotgun sequence.
ACCESSION   DAORFF010000529 DAORFF010000000
VERSION     DAORFF010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25551_length_2755_cov_3.727037"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PKK76_16870"
     CDS             complement(<1..590)
                     /locus_tag="PKK76_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70205.1"
                     /translation="MIMQKGINEILSQFKQDDYTVKLCSSLFSVVPFAPPFQFYNSLE
                     GAAGRMQATSDTVTKANALAASEDVKNAVWVADALDTSDKLIAGYAGVKNLLSLFSSS
                     SAPKARTFESDPQQAVDAGLKALGLAYMIQKLFAGSITEKVNAFRELPAGQEIGLYYA
                     TAEIALPFADNLVEGGASVVERLVGKHKGDIQAKFA"
     gene            complement(700..1995)
                     /locus_tag="PKK76_16875"
     CDS             complement(700..1995)
                     /locus_tag="PKK76_16875"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNL70206.1"
                     /translation="MSETAKLIFRDKQLELPVLTGTEDEIAVDIGKLRGSTGLITMDD
                     GYMNTGSCTSAVTFLDGEKGILRYRGIPIDQLAEKSTFLEVAYLLIYGELPSQKQYEA
                     FSTQVTRHTLIHEDLKRLYDGFPKDSHPMAILSSMICTLSGYYTDSSDPLNPRHREVS
                     IMRLLAKTPTIAAYSHKKSIGQPFVYPRNALSYVGNFLNMMFSVPAEDYEVDPIVEKA
                     LDLLLILHADHEQNCSTSTVRLVGSSRANIYATIASGVCALWGPLHGGANQAVIEMLE
                     LIRDDGGNVAKFVELAKDKNSTFRLMGFGHRVYKNFDPRARIIKKACDDVLNKLGVKD
                     PLLDIAKKLEEAALTDPYFVERKLYPNVDFYSGIIYRAIGIPTEMFTVMFALGRMPGW
                     IAQWKEMIEAPGTKIGRPRQVYTGYAERDYVPREKRPVD"
     gene            2091..>2755
                     /locus_tag="PKK76_16880"
     CDS             2091..>2755
                     /locus_tag="PKK76_16880"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL70207.1"
                     /translation="MRFVFLLAALCVFAGCSSLPSGAEPVREIHNQVYAEHEGKNLMA
                     DVFLPQSPGPHPAILLLHSGSWRHGSKSRMADVGRAFAREGFVAVAANYRLTNEARFP
                     AQIHDTRDALRWMKENHERLEIQPNAVGILGYSSGGHLALLAGLTGNEHRSILADVQA
                     IAVAGAPSNLETLVSNPAVYALLGGWKSDKPRAYSFASPVQYATPDDPPVLLMHGKYD
                     MIVP"
BASE COUNT          644 a          707 c          703 g          701 t
ORIGIN      
        1 tgagcgaatt ttgcctggat gtctcctttg tgttttccaa ccagacgttc caccacagac
       61 gctccgcctt ctaccaggtt atccgcgaac gggagggcga tctctgctgt agcgtagtag
      121 agaccaatct cctggcctgc aggcaattca cgaaatgcat ttactttttc tgtaatggaa
      181 ccggcgaaca acttctgaat catgtaagca agacccagag ccttcagtcc cgcatccaca
      241 gcctgctgtg gatccgattc aaacgtgcgc gctttgggag cgctcgaaga cgaaaacagg
      301 gagaggagat ttttgacccc tgcgtatcct gcgatcagct tgtcgcttgt atcgagcgcg
      361 tctgcaaccc agactgcatt cttcacgtct tcggaggcgg cgagtgcgtt tgcctttgtg
      421 actgtatcgc tggttgcctg cattctacct gccgcacctt ccagtgaatt gtagaactga
      481 aacggcggtg cgaacggcac cacggaaaac aggctggaac acagttttac tgtgtagtca
      541 tcctgtttga actgagatag tatttcattg attccttttt gcatgatcac tccttttgta
      601 aacagttaga gaatacaaac cgctcccgtg caggaaagca gattttcgga ggcgatttcg
      661 ccgcgcattc ccaggcgcgg ccggtagctc ttttctagct cagtctactg gacgcttttc
      721 gcgtgggacg tagtcacgct ctgcatagcc agtgtagacc tgacgaggac gaccgatctt
      781 tgttccgggc gcctctatca tctctttcca ctgtgcaatc cagccaggca tacgaccgag
      841 tgcgaacatg actgtaaaca tctctgttgg aattccaatg gcacgataga tgatgcccga
      901 atagaagtct acgtttggat agagtttgcg ctctacaaaa tagggatccg tcaaggcagc
      961 ctcttccagt ttctttgcaa tgtccagaag tggatctttc actccgagct tgtttagaac
     1021 gtcatcgcat gctttcttga tgatgcgcgc acgcggatca aaattcttat acacgcgatg
     1081 tccaaagccc ataagacgga atgtagaatt cttgtctttg gccagttcaa cgaacttggc
     1141 aacatttcca ccgtcgtcgc gaatcaactc gagcatctcg atgactgcct ggttggctcc
     1201 gccgtgaaga ggaccccaaa gagcacacac accactggcg atcgttgcat aaatgttggc
     1261 ccggcttgaa cccacgaggc gaacggtaga agtagaacaa ttctgttcgt ggtctgcatg
     1321 cagaatgaga agcagatcta gagccttctc tacgattgga tccacttcgt aatcttctgc
     1381 cggaacagag aacatcatgt tgaggaagtt gcccacataa gacagagcat tgcgcggata
     1441 aacaaacggc tgcccgattg atttcttatg cgaataagca gcgatcgttg gcgtcttggc
     1501 aagcaagcgc atgatggaga cttctctgtg acgcggattg agtggatcgc tggagtctgt
     1561 gtagtagcca gacaatgtgc agatcatgga actcaagatg gccattggat gagaatcttt
     1621 aggaaaacca tcatacaatc ttttgagatc ttcgtggatc agtgtatggc gtgtaacctg
     1681 ggtggaaaat gcctcgtatt gtttctgact gggtagctct ccgtaaatca gaagataggc
     1741 gacttcaagg aatgtggatt tttcagcgag ctgatcaata ggaatgccgc ggtatctaag
     1801 aatgcccttt tctccgtcca ggaaagtcac agcactcgtg catgaaccag tgttcatgta
     1861 gccgtcatcc atggtgatga ggccggtgga cccgcgcaat ttgccgatat ctactgcgat
     1921 ctcgtcctct gtgcctgtga ggaccggaag ttcaagttgc ttgtctctga agatcagttt
     1981 cgccgtttcg ctcatattgc ctcgcaaagc gtaatacgat gtccagtcag gtcgtgacaa
     2041 gtatattttt cgtgaatgcc tgaacgagcc ttctctatta ttctatgagc atgcgattcg
     2101 tctttctgtt agccgctctt tgcgtgtttg ctggctgctc ttccctgccg tctggcgcag
     2161 agccggttcg cgaaatccac aatcaggttt atgcagagca cgaagggaag aatctgatgg
     2221 cggacgtatt tctgccccaa tcgccaggcc ctcatccggc catacttctc cttcacagtg
     2281 gctcctggag acacggcagc aagtcacgga tggccgatgt tggaagagcg tttgcccggg
     2341 agggatttgt ggccgtggct gccaactatc gcctgacgaa cgaggcgcgc ttccccgctc
     2401 aaatccatga cacccgcgac gcacttcgct ggatgaaaga aaaccacgag agattagaaa
     2461 ttcaaccgaa tgcagtgggc atactgggat attcttcggg aggccacctc gcactcctgg
     2521 caggtctcac aggaaacgag cacaggtcca ttctcgcaga tgtccaggca attgcagttg
     2581 caggtgcgcc ttccaatctg gaaacactgg tatccaatcc ggccgtctac gcactgttag
     2641 gcggatggaa atcagataaa ccgcgcgcgt acagctttgc ttctcccgtg caatacgcaa
     2701 ccccggatga tcctcctgtt ttgctgatgc acgggaaata tgatatgatc gttcc
//
LOCUS       DAORFF010000530         2754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_25568_length_2754_cov_4.832901, whole genome shotgun sequence.
ACCESSION   DAORFF010000530 DAORFF010000000
VERSION     DAORFF010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25568_length_2754_cov_4.832901"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..681)
                     /locus_tag="PKK76_16885"
     CDS             complement(133..681)
                     /locus_tag="PKK76_16885"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNL70208.1"
                     /translation="MITAVGKTNQRKEQILSRGLAVTLHILDQGRELLAKLTQEDYSA
                     RSPLGASVGSHMRHILDFYRCFLRDEPAGSIDYDLRERNSESERLLDLARRELELVRE
                     RLRGLRWTENEPVQVKVEASHPVEFVTSTREREIQFLISHTVHHFALISYILAERGFA
                     VPADFGIAPSTLEFLRAESLAG"
     gene            707..1990
                     /locus_tag="PKK76_16890"
     CDS             707..1990
                     /locus_tag="PKK76_16890"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002735987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL70209.1"
                     /translation="MVQLLIRIFYRLVGNVKEHSLEHRLFSTISLLNGVANIGGSFAY
                     LLQGGSGLLQFAINAGTGVLFLGLYYLSRFRGIYYILYWPFNLLILGFLATLWFPNGG
                     SHGGSSYYLIPALMIATILARSGRTIVLVFLLYAAVAVALLFIEHQRPDLIAMPAVED
                     RFLDIVLNYPFVQIFSSGLVVVLSRNVHAEREKSDKLLLNILPQEIAEELKKTDQVAP
                     QSYDQVTVLFTDLVGFTRIAENLPPQDLLRELDSCFSSFDRIIKKHKIEKIKTIGDSY
                     MCAGGVPHRNKTNAIDCVLAALNMITYMKRLKADREAHGLPCWEIRLGIHTGHLVAGV
                     IGKEKFAYDIWGDTVNLASRMESSGVPMQVNISRATYELVSNLFDCEFRGAVQAKNKG
                     EIDMYLVRGVKAEFKGEDGKLNRKFVEAYKEVGAA"
     gene            2023..2385
                     /locus_tag="PKK76_16895"
     CDS             2023..2385
                     /locus_tag="PKK76_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70210.1"
                     /translation="MSDSEKAGSLYAKTMSHDTAGNDLRLRVAEIYGTYEQMRERTEI
                     MAASFLSDCYYGDRPARGAWLADITDFRSILCIGHDTEHSPICIDFAHGAKVILWDGY
                     AWREVAPGFAGFLRLFVG"
     gene            complement(2450..>2754)
                     /locus_tag="PKK76_16900"
     CDS             complement(2450..>2754)
                     /locus_tag="PKK76_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70211.1"
                     /translation="GIPVGVGLDKQAASSPPLMWGFSAGLVIDDTKFFAVTYGEFYDP
                     GTTRIAFPFYEGAPNPITGLTTLNATSLTQNGLISVPLEKVRGIYRGLGFSFNYKF"
BASE COUNT          651 a          653 c          733 g          717 t
ORIGIN      
        1 attccgctgg cacatgcgat gattcctgga accagggcga ctttgtgtgt cgcagccgcc
       61 attcccgtgg caatgcatgt tagatcttcg cttacgaagg tggagagtgc aatcagtacg
      121 taggcgagtc cgtcagccag caagactctc tgctctcaag aattcgaggg tggaaggtgc
      181 aatcccaaag tcagccggaa cagcaaatcc tctctccgcc aggatgtagg aaatcagtgc
      241 gaaatgatga actgtgtgcg aaatcagaaa ttggatttcg cgctctctcg tggacgtgac
      301 aaattcgact ggatgcgaag cttctacttt tacctgcaca ggctcgtttt cggtccaccg
      361 gagccctcgt agtctttcgc gtaccagttc cagctctcgc cgcgcgagat cgaggagtcg
      421 ttctgattcc gagtttcttt ctctcagatc gtagtcgatg cttccggctg gctcgtccct
      481 gaggaagcag cggtaaaaat ccagaatgtg tcgcatgtga ctgcccacgg atgcacccag
      541 cggagaacga gccgaatagt cctcctgcgt gagtttcgcc agaagttcgc gtccttgatc
      601 aagtatgtgc aacgttacag ccagtccacg ggacaaaatc tgctcctttc tttgattggt
      661 cttgccaact gcagtaatca ttacagtgtt tccgcgacga actgttatgg tgcaactgct
      721 aatccggatt ttttacaggc tggtgggaaa tgtgaaggaa cattccctgg agcatagact
      781 tttttccact atctctttgc tcaatggagt ggccaacatt gggggctcgt ttgcctacct
      841 tctacaaggg ggaagcggac tgctgcagtt tgcgatcaat gcaggcacag gcgttttgtt
      901 tctggggctg tactatctgt ccagatttcg cggcatctac tatattttgt actggccctt
      961 taacttgctc atcctgggat ttctggctac tttgtggttt ccaaacggtg gatcgcacgg
     1021 aggttcgagc tactacttga ttcccgctct catgattgcc acgattctcg ctcgaagcgg
     1081 aagaaccatc gtccttgttt ttcttttgta cgcggctgtg gccgttgcgc ttttgttcat
     1141 tgaacaccag cgtccggatt tgattgccat gccagctgtg gaagatcgtt tcctggacat
     1201 tgtcctgaac tatccttttg tgcagatttt cagttccggt ctggtcgtgg tcctatctag
     1261 gaacgttcac gcggaaagag aaaagtcaga caagctgctt ttgaatatcc ttccccagga
     1321 gattgcagag gaactcaaga aaacagatca ggtagcacct cagagctatg atcaggtgac
     1381 tgtgttgttt acggatcttg ttgggtttac tcgcatcgcg gagaatttgc cgccacagga
     1441 tttactcaga gagctcgatt cgtgcttttc ttcttttgat agaattataa agaaacacaa
     1501 gattgagaaa atcaaaacga tcggagacag ctatatgtgc gcgggcggcg tgccgcatcg
     1561 caacaagaca aacgcgatcg actgtgtact ggcagcgctc aatatgatta cgtatatgaa
     1621 acgcttgaaa gcggatcgcg aagctcatgg cctgccttgc tgggaaataa ggctggggat
     1681 tcacacgggt catcttgtag ccggagtcat tgggaaagag aaattcgcat acgatatctg
     1741 gggagatact gtgaatctcg cttctcgcat ggagagttcc ggtgttccca tgcaggtgaa
     1801 tatttccaga gcaacgtatg aactggttag caatttgttt gattgtgagt ttcgcggagc
     1861 ggttcaggca aagaacaaag gcgaaatcga catgtacctg gttcgcggtg taaaagcaga
     1921 gttcaaagga gaagacggaa agctgaaccg aaagtttgtg gaagcataca aagaagtagg
     1981 cgctgcttga ttcataccct gccgtctgaa ttgcaggact tgatgtctga ttcagaaaag
     2041 gcagggtcgc tttatgcaaa gaccatgagc catgacacag ctggaaatga tctgaggttg
     2101 cgcgttgcgg aaatctatgg tacctacgaa cagatgcgcg aacgcactga aatcatggct
     2161 gcttcttttc tttctgattg ctactatgga gatcgccctg cgcgaggagc ctggctcgca
     2221 gacatcacag actttcgttc tatcttgtgc attggtcacg acacagagca ttctcccatc
     2281 tgcatcgatt ttgcgcacgg agccaaagtg attctctggg acggatacgc atggagagag
     2341 gtggccccgg ggtttgctgg atttttgcga ttgtttgtgg ggtaggggcc cacaaacgaa
     2401 cgctttcttg cgcggttgcc gccgcgccgt gtcaccgggt gcctttttct tagaacttat
     2461 agttgaaact gaagccgagt ccgcgataaa taccgcgaac tttttccagt ggcaccgata
     2521 ttaggccatt ctgagtcaag gaagtcgcat tcaatgtggt caggcccgtg atcggattcg
     2581 gagcaccttc ataaaatgga aaagcaatac gtgttgtacc agggtcataa aactcgccgt
     2641 aagtcacagc aaagaacttc gtgtcatcga tcacgaggcc ggccgagaag ccccacatta
     2701 gaggcgggct agaagcagcc tgcttgtcca ggccaactcc cacgggaata cccc
//
LOCUS       DAORFF010000531         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_25712_length_2746_cov_3.858417, whole genome shotgun sequence.
ACCESSION   DAORFF010000531 DAORFF010000000
VERSION     DAORFF010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25712_length_2746_cov_3.858417"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2541
                     /locus_tag="PKK76_16905"
     CDS             <1..2541
                     /locus_tag="PKK76_16905"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL70212.1"
                     /translation="REDAPAIQTAVLPGGYESLLSAGKAAFARKDSAEARTRLEGALK
                     LHESEEVYEMLVRCCLAQKDFRAAADLVDQATLLYPANHRLLYLALRAFKGTGQYDRA
                     VEYGERAALRDPDNTDNLMHLADCYRLLKKSSRAQDAIRRAEKKQASPDLMRKLKGLL
                     GVAVLAIALTPSLRAESPIQLETSRDQQWQITSDEIDPEAETRAFFSEARTSDQTVVW
                     LPVQVPGERRGAKPGTIWLRTTLNLKEKPTSSLAFRAGIIRDRDRVFINGNLIGSTGE
                     WDASAPQAFDRSRLYEVKPDVFRTGDNLILIQIQGTPELPPAIRFGPLSLGTIQDALT
                     RQASTAYMHGALTVVYLTIASYFLFLFLKRSEERDNLYFGLFTILVAIFTFLQSELKY
                     QIQLSFMTFKKIELIAQFASVPAYFAFIQAFFPERRERDRGVLKVIGLSGYGIIALAC
                     ILVAATSNLSIWIFVRNYGVTTGCILLVVGGAGLVYNRLRAFERDAFYFTGGLIVLAG
                     GIIFAYLSETGILHLPNVLDGFLLIFVIINAMTLANRILRLTAQVEEMNADLERRVQR
                     RTEELGESLEKVRALKESQDADYFLTTLLLSPLSGAKLTESGMTPSILIKQKKSFDFR
                     KWRAEIGGDICVVDELPLKGRRYAIFMNGDAMGKSMQGAGGALVLGTVFRSLITRTQK
                     AERFQNQTPQEWLRDCFLELQTVFVTFDGSMLVSAVFGLIDLESREMYHVNAEHPTSV
                     LLRNGKASFVPTLHPVMKIGVRGMEDKMQVEQLLLQSGDILIAGSDGRDDVLLGYEPD
                     GTRIINADESLFLRIVENCGGSLGKIYESIQASGGITDDLSLLAIEVS"
BASE COUNT          686 a          699 c          710 g          651 t
ORIGIN      
        1 agagaagatg cgcccgccat acagaccgcc gttcttcccg gcggatacga atctcttctt
       61 tctgcaggca aagctgcttt cgcgcgaaag gattcagcag aagcacggac cagactggaa
      121 ggcgcactca aacttcacga aagcgaagaa gtctatgaaa tgcttgtgcg atgctgcctc
      181 gcacagaagg attttcgcgc ggcggcagac ctggtagatc aggcaactct actctatcct
      241 gccaatcaca ggcttttgta tctggctctc cgtgcattca agggcacggg tcagtacgac
      301 cgggccgttg aatacggaga acgcgctgcg ctgagagatc cggacaatac ggacaacttg
      361 atgcatctgg cggattgcta cagactactc aagaaatcat ctcgcgccca ggatgcgatt
      421 cgccgcgcag aaaaaaagca ggcttccccg gatcttatgc gaaaactcaa aggtttgctg
      481 ggggtcgcag tgctggcaat tgctcttacg ccatccttgc gcgcagaatc tcccatccag
      541 ctagaaacat cacgcgacca acaatggcaa ataacgtcag acgagattga tccggaagca
      601 gaaaccagag cctttttctc agaagcgcgc acgagtgacc agactgtagt ctggcttccg
      661 gtgcaggtgc ccggagaaag aagaggcgca aaacctggca ccatctggct tcgcacgact
      721 ctcaatttga aggaaaaacc cacatcgtct ctagcctttc gcgctggaat cattcgcgac
      781 cgggatcgcg tgtttatcaa tggcaacctc attggcagca caggagaatg ggacgcgagc
      841 gctcctcagg catttgacag aagtcgtctg tacgaagtga agccggatgt atttcgcacc
      901 ggtgacaatc tgatcttaat tcaaatccag ggaactccag aactccctcc tgccattcgg
      961 ttcggtccac tctcccttgg aacgattcag gacgctttga ctcgtcaggc aagtactgcc
     1021 tacatgcacg gagctctcac tgtcgtctat ctgaccatcg cttcgtattt tcttttcttg
     1081 tttctgaagc gcagcgaaga gagggacaac ctttactttg gtttatttac gattctcgta
     1141 gccatcttta catttcttca gagcgaactt aagtatcaga tccaactgtc tttcatgaca
     1201 ttcaagaaga tagaattgat cgcacagttc gcttctgtgc cggcctactt tgctttcatc
     1261 caggcgttct tcccggaacg gagggagagg gacagaggtg ttttgaaagt catcggtctt
     1321 tcgggctacg ggatcatcgc actggcctgc atccttgtgg ctgcgacttc gaacctgagc
     1381 atatggattt tcgtgcgaaa ttacggagtt acaaccggat gcattctact ggtggtgggt
     1441 ggcgcgggcc tcgtatacaa tagactgcgc gcgttcgaac gcgacgcgtt ctacttcaca
     1501 ggcggactga tcgtgctggc gggcgggatc atattcgcct atttgtcaga aaccggaatc
     1561 cttcaccttc ccaatgtgct cgatggcttc ctgcttatct tcgtaatcat aaacgccatg
     1621 actcttgcaa atcgcattct tcgccttaca gcacaggtgg aagaaatgaa cgcggatctg
     1681 gagagacgcg ttcagaggcg cacggaagag ctcggagaat ctctggaaaa agtgcgagca
     1741 ttgaaagaaa gccaggacgc agactatttc ctgactacac tcctactctc tcctctttca
     1801 ggcgcaaaac ttaccgaaag cggaatgaca cccagcatcc tgatcaaaca aaagaagagc
     1861 ttcgacttta gaaaatggcg cgctgagatt ggcggggata tttgcgtagt ggatgaactt
     1921 cctctcaaag gaagacgtta tgcgattttc atgaacggtg atgcaatggg caaatccatg
     1981 cagggcgccg gtggagctct ggtgcttgga acggtgtttc gtagcctcat cacaagaacg
     2041 cagaaggcag aaagattcca gaaccagact ccacaggagt ggctgagaga ttgctttctg
     2101 gagcttcaaa ctgtcttcgt cacgtttgat ggaagcatgc ttgtttctgc agtctttggt
     2161 ctcattgatc tggaaagcag ggaaatgtat cacgtaaatg ctgagcatcc cacctctgta
     2221 ctgttgcgaa acgggaaagc atcctttgtt ccaacactcc accctgtcat gaagatcggt
     2281 gtgcgaggga tggaagacaa aatgcaagtg gagcaactct tgcttcagag cggagacatt
     2341 ctaattgcgg gcagtgacgg cagagatgat gttcttctgg gctacgaacc ggacggcacg
     2401 cgcatcatca atgcagacga gtccctcttt ttgcgcatcg tggaaaactg cggcggaagc
     2461 ctgggaaaaa tttacgaatc cattcaggca agcggaggca tcacagacga tttgagtcta
     2521 ctggccattg aagtgagctg actctcttac tcagttccac gaatcttttt taccggaacg
     2581 ttagtcgccc atgcctgcga cttttagaaa accgtgagcg aacgtatagc catctccggg
     2641 ccagcgcgct gtgatgaggt ttccatctac aaccgcaaag ccaggtttac gattctcctc
     2701 gctgtctctg aacatgggtg tggggccttc tgaaaaatca gaagga
//
LOCUS       DAORFF010000532         2742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_25769_length_2742_cov_3.477112, whole genome shotgun sequence.
ACCESSION   DAORFF010000532 DAORFF010000000
VERSION     DAORFF010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25769_length_2742_cov_3.477112"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2102
                     /locus_tag="PKK76_16910"
     CDS             <1..2102
                     /locus_tag="PKK76_16910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNL70213.1"
                     /translation="SCDVIIVTGDAYVDHPSFGMAIVGRLLEAQGFRVGILSQPDWTS
                     VEDFKKLGKPNLFFGITAGNMDSMVNRYTSDRKLRNDDAYTPGGEAGKRPDRSVLVYT
                     QRCKEAYKKVPTVIGGIESSLRRVAHYDYWSDQVRRSILLDAKADLLVYGNGERQIVE
                     IAHRLANKEDIQSITDIRGTAFLRASIPENFTIIDSTHIDEPGGKDDGKTNPYLYVPG
                     VPSPGAAEPAAKAEILTEGHEYKHPPLVARENTVIRLPSYDALKEDPVLYAHSSRIVH
                     LETNPGNARALLQNYGDRELWLNPPPIPLTTKEMDGIYEQPFSRMPHPSYQGAKIPAF
                     EMIQHSVTIMRGCFGGCTFCSITEHEGRIIQNRSESSVLREIEKIRDTSPSFTGYISD
                     LGGPTANMYRLACKSRTIESACRRPSCVYPDVCPNLNTDHSSLVNLYRRAREIPGVKK
                     ILIASGVRYDLAVKSPEWIKELATHHVGGYLKIAPEHTEKGPLSKMMKPGIGAYDRFK
                     ELFDRYSAQAGKEQYLIPYFIAAHPGTTDEDMLQLALWLKKNGFRADQVQAFLPSPMA
                     SATAMYYSGKNPLRKVTHTSEEVPIPRGLKIRRLHKAFLRYHDANNWPVLREALRRMG
                     RGDLIGNSKHHLIPTYQPLGTGLAHEGRRGPRSAPSTKDRGNKILTQHTGLPPRAHGP
                     SVSSNRGVSRSRPKKKR"
     gene            2133..>2742
                     /locus_tag="PKK76_16915"
     CDS             2133..>2742
                     /locus_tag="PKK76_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNL70214.1"
                     /translation="MSLLHPTRETWAQFDPATRKIFQSTVQFFETKGKRALKKEDHEV
                     IWYTDFLDFLKREHVFHYFLTPSAYGDSTECRWDSARNTAYSEILGFYSLSHWYTWQV
                     SILGLGPIWMSKNEEIKKKTAAKLKDGGIFAFGLSEKEHGADLIGSDMMLVPKDGAWI
                     AQGDKYYIGNGNCAAYVSVFGKTADRREEFVFFAVESNHPKYE"
BASE COUNT          733 a          667 c          687 g          655 t
ORIGIN      
        1 attcttgcga tgtcatcatt gtaaccggcg atgcgtatgt cgatcatccg agctttggaa
       61 tggccattgt gggccgcctt ctagaagcgc aggggttcag agtagggatt ctctctcagc
      121 cggactggac ttcagtagag gatttcaaga aactgggtaa gccaaatttg tttttcggaa
      181 tcaccgcggg caatatggac tcgatggtga atcgttatac ttccgatcga aagcttcgca
      241 atgacgacgc ctacactcct ggtggagagg cagggaagcg accggatcga agtgtgctcg
      301 tgtacactca acgttgcaag gaagcatata aaaaagttcc aacagtgatc ggaggaattg
      361 agtcgagtct tcgcagagtt gctcattacg attactggtc tgatcaggtc agacgttcca
      421 ttctactgga tgcgaaagca gatcttctcg tctacggaaa cggagaaaga cagatcgttg
      481 agattgcaca caggcttgcg aacaaagagg acattcagag cattacagac attcgaggca
      541 ctgcctttct tcgtgcatct attccagaaa attttacaat cattgattcc actcacattg
      601 atgaaccggg tggaaaggat gacggaaaaa ccaatccgta tctctatgta cctggtgttc
      661 cttctccggg cgcagccgag cccgcagcaa aggcggagat tctcacggaa ggacacgaat
      721 acaagcatcc tcccctggtt gcgcgagaaa ataccgttat acgacttcca tcttacgacg
      781 ccctgaaaga agacccggta ctgtacgctc attcttcgag aatcgttcat ctggaaacaa
      841 atccaggaaa cgctcgcgca cttttacaga attatggaga tagagaactc tggctcaatc
      901 caccgcctat tcccctgact acgaaagaaa tggacggtat ttacgagcag ccgttcagca
      961 gaatgcccca tccttcgtat cagggtgcga aaatccctgc ctttgaaatg attcaacatt
     1021 ccgtcacgat catgcgcgga tgtttcggcg gttgcacctt ttgttccatc acagagcacg
     1081 aagggcgaat cattcagaat agatcagaat cttccgtact gcgcgaaata gagaaaattc
     1141 gagatacatc gccttcgttc actggctaca tttcagatct cggcggtcct acggcgaaca
     1201 tgtatcgcct ggcgtgcaaa tctcgcacaa tcgaaagcgc atgcagaaga ccttcctgcg
     1261 tttatccgga cgtttgcccc aatctaaaca cagatcacag ttcgcttgtg aatctgtaca
     1321 gacgtgcgcg cgaaatccct ggagtcaaaa agatactgat tgcttccgga gttagatacg
     1381 atcttgccgt gaaatcgcct gagtggatca aggaactggc aacgcatcat gtgggtggtt
     1441 atctgaaaat tgccccggaa catacagaga aagggccact gtcaaaaatg atgaagcctg
     1501 gaattggagc gtatgatcgc ttcaaggaat tgtttgatcg atattctgcg caggcgggta
     1561 aggagcaata tctcattccg tatttcattg cggctcatcc aggtaccaca gacgaagaca
     1621 tgcttcaact tgccctctgg ttgaagaaga acggatttcg cgcagatcaa gtgcaggctt
     1681 tcttgccttc gcctatggcg agtgctactg cgatgtacta ttcaggaaaa aatccgcttc
     1741 gcaaagtcac gcacacaagc gaagaggttc cgattccacg cggtctaaaa attcgcaggc
     1801 ttcacaaagc gtttctcaga taccatgacg cgaataactg gcccgttttg cgggaggctc
     1861 tccgtcggat gggtcgcggg gatttgatcg gtaactccaa acatcatttg attcctactt
     1921 accagccgct gggcaccggt ttggcgcacg aaggaaggag aggaccacga tccgctccga
     1981 gcaccaaaga cagagggaac aagattctga cccagcatac cggacttccg ccgcgtgcgc
     2041 acgggccttc cgtttcgtcc aaccgtggag tctcccgatc acgaccaaag aaaaaacgat
     2101 gacttgcgcg cggttgattt tgaaactcgc ctatgtcact tttgcatcca accagggaaa
     2161 cctgggccca gtttgaccct gcgacccgta aaatctttca atccacagtt cagttctttg
     2221 aaaccaaagg gaagcgcgcg ctgaaaaaag aagatcacga agtgatctgg tacacagact
     2281 tccttgattt cctgaagcgt gagcatgtgt tccattattt tctgacgcct tctgcatacg
     2341 gggattctac cgaatgtaga tgggactctg cgcgaaacac tgcttatagt gagatactcg
     2401 gtttttattc tctctcccac tggtatacgt ggcaggtttc catcctgggt ctcgggccca
     2461 tctggatgag taaaaatgag gaaatcaaaa agaagacggc cgcgaaactg aaagatggcg
     2521 gcatttttgc gttcggtctg tctgaaaaag aacatggcgc ggacctgatt ggttctgaca
     2581 tgatgctggt tccaaaagat ggcgcctgga ttgctcaggg agataagtac tacataggaa
     2641 atggaaattg cgccgcgtat gtatctgtgt tcgggaaaac agcggacaga cgtgaggaat
     2701 tcgtcttctt cgccgtagaa agcaaccatc cgaaatatga gt
//
LOCUS       DAORFF010000533         2738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_25842_length_2738_cov_2.769288, whole genome shotgun sequence.
ACCESSION   DAORFF010000533 DAORFF010000000
VERSION     DAORFF010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25842_length_2738_cov_2.769288"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PKK76_16920"
     CDS             complement(<1..556)
                     /locus_tag="PKK76_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70215.1"
                     /translation="MQKLKTVTSLESILEAWDLYANRKFEAIVSHEEYSHSPSKDLRD
                     LARLGQLEIARNTTDPEGELFAPLVQAMRQYWKGHYSDACALLGQWLETREYHTAMIL
                     DRFLECAFMCDQYRPAFQILNKFIKIPYYRDRLADDIVRSAHHAGLDKEAVGLYRDLA
                     GSIRDQSVHQKAALSMVKLGLFRDA"
     gene            complement(613..879)
                     /locus_tag="PKK76_16925"
     CDS             complement(613..879)
                     /locus_tag="PKK76_16925"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNL70216.1"
                     /translation="MRFVIQGRVQGVGFRQFTRQEARSLDIEGFVTNRPDGSVECLAR
                     GSRESMDQFQSKLKKGPPLSRVESIVATPETTFPAGIKAGFQIL"
     gene            971..1663
                     /locus_tag="PKK76_16930"
     CDS             971..1663
                     /locus_tag="PKK76_16930"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNL70217.1"
                     /translation="MKRLSVNLDHVATLREQRHTPYPDLTRAADLARAGGADGITLHL
                     RGDRRHIQESDLAALSGGLLPVTLEIACTPENMEILRKYKPFAVTFVPERPEELTTEG
                     GMNLSVASAMLARLIPEIRSLGIRVCLFLEPDEKALRLAKDLGATMVEIHTGGYAEGR
                     VSVEEILQAADAGDSMGLEINAGHGLTVENARRLGQGKIHEFSIGHSIICRSVMVGLE
                     EAVREMKAAIQG"
     gene            1696..>2738
                     /locus_tag="PKK76_16935"
     CDS             1696..>2738
                     /locus_tag="PKK76_16935"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNL70218.1"
                     /translation="MEFLNIIIDRVDSTNVFNVLRGRMPSRESHLHSMMDNDLIQEYL
                     LEIERLSKIGTSLTGQERDGIFDLTGNLRRMGESFFLQFFPSAVQEMLRSAENGFLFL
                     HTDEQLRQIPWELLHDGVCFLADKFYIGKNISGHWRDRQREERENLRVLIACDPTEDL
                     EWARREGEGLFEALNSEVDPERLDIRFMSGRDITKLSLLDAMKDRDVVHYAGHVDPAR
                     TPGESGWPLSGGKILRTREIEKAGFSPDLVFANGCSVQRSLGESDVASAFLRAGIANF
                     IGTNWEIRDSQNTLDFAVHFYRSIFEEKSLGESLFEARQFARRTYPPADLTWANYVLH
                     GNPMARIYRSNRR"
BASE COUNT          644 a          637 c          760 g          697 t
ORIGIN      
        1 ccgcatcgcg aaagaggccc agcttgacca tggatagagc tgccttctgg tgaaccgact
       61 ggtctcgaat cgaccctgct agatcacgat agagacccac agcctctttg tcgagccccg
      121 cgtggtgagc gctacgcacg atatcatctg ccagtctgtc tctgtagtaa ggaattttta
      181 taaatttgtt caatatttga aaagcaggtc tgtactggtc gcacataaac gcgcattcaa
      241 gaaagcggtc aaggatcatg gcggtatgat attctctggt ttcgagccac tgacccagga
      301 gggcgcatgc gtcgctgtag tggcccttcc agtattgcct catggcctgg acgagaggag
      361 cgaagagctc gccctcagga tctgtggtgt tgcgagcgat ctcgagctgt ccaagtcttg
      421 ccagatcgcg cagatctttg gagggagagt gcgagtattc ttcatgcgaa acaatcgcct
      481 cgaactttct attcgcatag aggtcccagg cttctagtat ggattcgagg gaagtaaccg
      541 ttttgagttt ttgcatggcc gcctgctatt tgcttcggtg ggaagcggcc tttctataac
      601 cgttttttgg ccttacagga tctggaatcc ggccttgatt cctgctggaa atgtggtttc
      661 tggagtagcc acgattgatt ctactctgga tagagggggg ccttttttaa gcttcgattg
      721 gaactggtcc atgctttccc ggctgcctcg tgccaggcat tctacgcttc catcgggtct
      781 gttggttacg aagccttcta tgtccaggct tctggcctcc tggcgagtga actggcggaa
      841 cccaacgccc tggactctgc cctggatgac gaatcgcact gtctgtttgc tttccactgg
      901 gagccatcga aagcagggtg caaaaaaaat caagatttgc gcggtggttg cacctggatc
      961 tactgaccct gtgaagcgtc tgtctgtaaa cctggaccat gtggccactc tgcgagagca
     1021 gcgtcatact ccgtatcctg acctgacccg cgcggcggat ctggcgcgag cgggcggtgc
     1081 ggacggaatc actcttcacc ttcgcggaga cagaaggcac attcaggaat cggatctggc
     1141 ggccctgtcc ggtggactcc ttccggtcac tcttgaaatc gcatgcacgc cagagaacat
     1201 ggagatacta cgtaagtaca aaccttttgc tgtgacgttt gttccggaaa gaccggaaga
     1261 gcttacaacg gaaggcggaa tgaacttgag cgttgcctcg gccatgctcg ctcgtctgat
     1321 tcctgagatt cgttctctcg gaattcgcgt atgcctgttt ttagagccgg atgaaaaggc
     1381 tcttagactg gcaaaggatc tgggcgccac tatggtggag atccacacag gtggttacgc
     1441 agaagggcga gtgtctgtag aggaaattct tcaggcagcg gacgcgggcg attcgatggg
     1501 actcgagatc aatgcagggc atggtctgac tgtagagaat gcgcgtcgtc tgggtcaggg
     1561 aaagatccat gagttttcga tcgggcattc catcatttgt agatctgtga tggttggcct
     1621 tgaggaagct gtgagagaaa tgaaggctgc cattcagggt tgaatcgacc tgcaaagtaa
     1681 ataccggcaa caaaaatgga attcctgaat atcatcatag atcgagtgga ttcgacaaac
     1741 gtcttcaacg tgttacgcgg acgcatgcct tctcgagaga gccaccttca ttcgatgatg
     1801 gacaatgatt tgattcagga atatcttctt gaaattgaac ggctgagcaa aatcggcaca
     1861 agcctgaccg gtcaggaacg agacggaatt tttgatctaa cgggaaacct tcgcagaatg
     1921 ggagagtctt tcttccttca gttctttccc tctgcagtgc aggaaatgct tcgatctgcg
     1981 gagaacggtt ttcttttttt gcacacagat gagcaactca ggcaaattcc ctgggagcta
     2041 ctgcacgatg gagtctgttt tcttgcggat aagttttaca ttggaaaaaa tatttcagga
     2101 cactggagag atcggcagag agaggagaga gaaaatcttc gcgtgctgat tgcatgcgat
     2161 ccaacggaag atctagaatg ggccagaaga gaaggagaag gtttgtttga agctctcaac
     2221 agcgaggtgg atccggaacg tctggatatt cgcttcatgt ctggtcggga tatcacaaag
     2281 ctctctcttc tagatgcgat gaaggatcgc gacgtggttc attacgcagg ccatgtggat
     2341 ccggccagaa ctccaggaga aagtggatgg cctctttccg gaggaaagat ccttcgcacg
     2401 cgcgaaatag aaaaggccgg gttttcaccg gacctggtgt ttgcaaatgg atgttccgtt
     2461 caaaggtctc ttggtgaatc ggacgtggct tctgcatttc tgcgtgctgg aattgcaaac
     2521 ttcattggca ccaactggga aattcgagat tctcaaaaca ctctggattt cgcggtgcat
     2581 ttctatcgaa gcatctttga agaaaagtct ctgggagaat ctctttttga agccaggcaa
     2641 tttgcgcgta gaacgtatcc acctgcagat cttacatggg caaactacgt tctccatggc
     2701 aacccgatgg cgcgtatata tagaagcaat cgtcggtt
//
LOCUS       DAORFF010000534         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_25857_length_2737_cov_2.853468, whole genome shotgun sequence.
ACCESSION   DAORFF010000534 DAORFF010000000
VERSION     DAORFF010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25857_length_2737_cov_2.853468"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..855)
                     /locus_tag="PKK76_16940"
     CDS             complement(151..855)
                     /locus_tag="PKK76_16940"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004490139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNL70219.1"
                     /translation="MIQNILVIAAHPDDEILGCGGTMARHAGEGDSVHVLILAEGLTS
                     RSEERNRGSHETALSELAQTAKGANEILGVRSCELLDFADNRMDGADLLDVVKAVEKR
                     IDALRPDVIYTHFPGDLNVDHRIVSQAVATAARPVPGQTIKEIYFFEVQSSTEWQINP
                     SANGFLANHFVSLFPTPDSRYMSKKLEALALYSSEMRDFPHARSVRAVEALAALRGAT
                     IGTEAAEAFMTGRVIR"
     gene            959..1801
                     /locus_tag="PKK76_16945"
     CDS             959..1801
                     /locus_tag="PKK76_16945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL70220.1"
                     /translation="MLAVMGTDHGIQYQKPPRNLSRDVRRNHGMKEDVYKFWEEQSKK
                     GIKSGTKDLILDELERKAISSFLTNGLHVLEVGCGDGRNSIEMRQHFDVHVDAFDYSP
                     GMVSAATENARQALISDINFFVHDLNEVDSLEKKYDLVVSKRALINLSDYNTQVEVIK
                     RISKVLKPGGLFLMCESSKRGLDTINTARVSLELDQITPPWHNVYIDDDKLASEALPL
                     ELAQRLDFSSTYYFLSRVVNAKMAKMNGSLPDYDSPINKWALQLPVFGQMGQTILWIW
                     RNPH"
     gene            complement(1831..>2737)
                     /locus_tag="PKK76_16950"
     CDS             complement(1831..>2737)
                     /locus_tag="PKK76_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNL70221.1"
                     /translation="GRSKVAFCGFHGWHDWYLAANLGDEANLAGHLLPGLSPNGVPSN
                     LRDTVYPFNYNDYAQLESIVSSQQIGVIMMEVSRNLGPEPGYLEKVRKLATDRGIVLI
                     FDECTSGFRQTLGGLHKMYGVEPDMAMFGKALGNGYAITACIGRREIMEAAQTSFISS
                     TFWTERIGPAAGLKTLEIMERTRSYEQITATGRSISERWEKLAEKHSLEIDVFGLPAL
                     TGFVFKSENHLPYRTLLTQEMLARGFLAGTSAYACTAHTPEIIDAYFNELDPIFATIR
                     ECEEGRDVSTLLKGPVVHSGFKRLN"
BASE COUNT          689 a          708 c          653 g          687 t
ORIGIN      
        1 atcctgcttt aagatctgcg cgagcgacgc ccgcatcgat ggtctcatat ggaaggtgaa
       61 tgtcctggtt gtgattggaa ccgatatctc caatcaggaa cgtgcgcttg ccgccaattt
      121 cgcggctgcc aactttgatt gttctttctt tcatctaatt acccggcccg tcatgaatgc
      181 ttctgctgct tctgttccga tagttgcacc gcgcagagct gccagcgctt cgaccgcacg
      241 cacggaacga gcgtgaggga aatcccgcat ctcagacgaa tacagagcga gagcctcaag
      301 cttttttgac atgtatctgc tgtctggagt tggaaaaaga gaaacgaaat gattcgcaag
      361 gaagccgttt gcagaaggat tgatctgcca ttcagtactt gattgtactt caaaaaagta
      421 aatttccttt attgtctgac cgggcacggg tctcgcggct gttgcaacag cctggctgac
      481 aattctgtga tccacattca agtcgcccgg aaagtgcgta tagatcacgt cggggcgcag
      541 cgcatcgatt ctcttttcga ctgctttgac aacgtccagc aaatccgctc cgtccattct
      601 gttgtctgca aaatcgagca gttcgcaact cctgacgccc agaatctcgt tcgccccttt
      661 tgcagtttgc gcaagctcgc tcaacgcagt ttcatgagag cccctgtttc gctcttcgct
      721 tctgctggtc agtccttctg ccaggatcaa aacatgaaca ctatcgcctt cgcctgcatg
      781 acgggccatt gtcccgccac atcctagaat ttcatcgtca ggatgagccg ctatgacaag
      841 tatgttctgt atcattccac tttcctttca aaaaacatca cacgatcaga ggaaaatact
      901 tgggttaccc tggccgcgac gcctcctcgc gcaacaaaaa atactggaaa tccatgacat
      961 gctggctgtt atgggaacag atcatggaat tcaataccaa aagcctccga gaaatttatc
     1021 gcgcgatgtc agaaggaacc acggcatgaa agaggacgtc tacaagttct gggaagagca
     1081 atccaaaaag ggaatcaagt ctggcacaaa agatttgatc ttggatgaac tagagaggaa
     1141 ggcgatttca tcttttttaa caaatgggct tcatgtcctt gaggtcggtt gcggggatgg
     1201 gagaaactcg attgaaatgc ggcagcactt cgatgtgcac gtggatgcat ttgattattc
     1261 gccgggcatg gtgagtgccg ccacggagaa cgcgcgccag gccttgatta gtgacattaa
     1321 cttctttgtc catgacttga atgaagttga ttctttagaa aagaaatacg atctcgttgt
     1381 ctctaagaga gcgctcatca atttgagcga ctacaatacc caggttgagg tcataaaaag
     1441 gatttctaaa gttctcaaac caggtggatt atttttgatg tgcgagagca gcaaacgggg
     1501 acttgataca atcaacacgg cccgcgtctc attggaattg gaccaaatca cacccccttg
     1561 gcacaatgtc tatatcgacg atgacaaact cgccagtgaa gcattgccat tagagttggc
     1621 gcagcgactt gatttttctt ccacctacta tttcttgagt cgcgtagtga atgccaagat
     1681 ggcaaaaatg aatggaagcc tcccggacta cgattcgcct ataaacaaat gggccttgca
     1741 actccccgtg tttggccaga tgggccagac gatattatgg atatggagaa atccgcacta
     1801 attgcccatg tcgcgtgggt agtacatcaa ctaattcaac cgcttgaaac cgctgtgaac
     1861 cacgggtccc ttcaagagag tagacacatc gcgcccctct tcacactctc ggattgttgc
     1921 gaagattgga tccagttcat taaaatatgc gtcgatgatt tcgggcgtgt gtgcggtgca
     1981 ggcatatgcg cttgttcccg ccagaaatcc ccgcgcaagc atttcctgcg tcagcagtgt
     2041 gcgataaggg aggtgattct ctgatttaaa aacaaagccg gtcagcgcag gaagtccgaa
     2101 tacatcaatc tccagcgaat gtttttcggc caatttttcc caacgctctg agatacttct
     2161 gcccgtcgcc gtaatctgtt catatgacct ggtgcgctcc atgatttcca gtgtcttgag
     2221 cccggcagct ggtccgattc tttccgtcca gaaggtactg ctgataaacg aagtctgcgc
     2281 ggcttccatg atctcacgcc ggccaatgca cgctgtgatt gcatatccgt tgccgagcgc
     2341 ttttccaaac atggccatgt caggctccac gccgtacatt ttatgaagac cgcctaacgt
     2401 ttgacgaaat ccggaagtac actcatcaaa gatcaaaaca attccacgat ccgttgcaag
     2461 cttgcgcacc ttttccagat atccaggttc aggcccgagg ttgcgcgaca cctccatcat
     2521 gatcacaccg atctgctgcg aagaaacaat ggactcgagc tgtgcgtaat cgttgtagtt
     2581 aaacggatac acggtgtcac gcagattgga aggcactcca ttcggagaaa ggcccggcag
     2641 cagatgcccc gccagattgg cttcgtcccc cagattcgct gcgaggtacc agtcatgcca
     2701 tccatggaaa ccgcaaaacg ccactttcga tcttccg
//
LOCUS       DAORFF010000535         2736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_25866_length_2736_cov_3.397613, whole genome shotgun sequence.
ACCESSION   DAORFF010000535 DAORFF010000000
VERSION     DAORFF010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25866_length_2736_cov_3.397613"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PKK76_16955"
     CDS             complement(<1..183)
                     /locus_tag="PKK76_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70222.1"
                     /translation="MFKSVRLTRLGLINLILFTLFIGAFLSPLIYSGFRSMVLDHQSS
                     ADPIPESDQKAAIALQQ"
     gene            complement(213..1385)
                     /locus_tag="PKK76_16960"
     CDS             complement(213..1385)
                     /locus_tag="PKK76_16960"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNL70223.1"
                     /translation="MGYQFIDKQQDLDLAVSVMGRSQYVSVDTESSSFYTYESELCLI
                     QISASGKNYLIDPLANLTVDGLAEIFANPSIVKVMHTAASDISELRRLRKFKFENLFD
                     IMVGCRMLGFVSCSLVSLVKHYTGRDLEKTEQKSNWKKRPLTASQMEYAHRDTIYLEE
                     IMKAMKVEIEKFGFADEFAEDMIRIIKESYPVERIYDHDSWKHVPGAFQLSPIGRGFL
                     KALLEIRESRARKDNIAPFRLGSNDALLNVAERMPDSISELSTFGLHPDFVRKEGERI
                     LEMRNKSPQITNADSPREKRKPPDPALARLKKWRKDVAEYRGFEPSIILSNRQLEAIM
                     EARPSTMEDLARLDLLTDWKLEHYGPMVLSVLAGKEPPLGDLKRLPPERRRPQISG"
     gene            1460..2005
                     /gene="lepB"
                     /locus_tag="PKK76_16965"
     CDS             1460..2005
                     /gene="lepB"
                     /locus_tag="PKK76_16965"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNL70224.1"
                     /translation="MALRNRDLVPEKRAPVKKSVIVAGLVAAAILALMLRLVVHAVWI
                     FPLRVGTASMEPAVKKGQTVYVVKRVELQFGDIILYKHPDNPEVRLLGRITGLPGDAI
                     EMKEKQLLRNGAPVQDAWQIRTQTGYSLPGSVFPRDSFAERKITTGKYFVLCDLRTEC
                     LDSRLLGEISREMIVGRIRPD"
     gene            complement(2010..>2736)
                     /locus_tag="PKK76_16970"
     CDS             complement(2010..>2736)
                     /locus_tag="PKK76_16970"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNL70225.1"
                     /translation="VGHGRRSYLEVRAYTQKRTAYGKKIIELPAVLRTLARMQVLQTV
                     VEFAVMRNYDLNAKDHVLSKMLTPLLKYASTVLATHLTHEGILVLGGNGILGDFSILP
                     RLHNDAIINETWEGTHPLLCEHALKAYGRPNVYNAFKEMLSEAARNVGSLPLAARAQA
                     LISSRINDCEKQSDFWVDSHRTEFCDLLYAAFSLSEMIQDSAGSQAFRPHTEAYAELL
                     IEKYSGETLNGFFSDPSQMAAIL"
BASE COUNT          722 a          674 c          686 g          654 t
ORIGIN      
        1 ctgctgcaag gcgatggccg ccttctgatc gctctctgga attggatctg cgctcgactg
       61 gtgatccagg accatgctgc gaaagccgct gtaaatcaaa ggactcaaga atgccccgat
      121 gaaaagagta aacaggatga ggttgatcag cccgagacga gtcagacgaa cagatttgaa
      181 catgaaccca gattgtgcga agggcccggg tatcaacctg aaatttgcgg gcgtcgtctt
      241 tctggaggaa gccttttcaa atcgcccaga ggtggctctt taccggccag aacagacagt
      301 accataggac cataatgctc cagcttccag tccgtaagta aatccaggcg ggccagatct
      361 tccattgtag aagggcgcgc ttccatgatt gcttcaagct gcctgttgga taaaatgatg
      421 gaaggttcaa atccgcgata ctctgccaca tccttgcgcc attttttcaa acgagccaga
      481 gcaggatcgg gtggtttgcg tttctcacga ggcgaatctg cattcgtaat ctgcggcgat
      541 ttgttgcgca tttccagaat tcgttcccct tctttgcgaa caaaatctgg atgaagtcca
      601 aaagtagaca gctcgctgat gctatcgggc attctttctg ctacgttcaa aagagcgtca
      661 ttggaaccaa gacgaaacgg agcaatgtta tctttacgcg cgcgtgattc gcgaatctca
      721 aggagcgctt tgaggaagcc gcgaccgatc ggggaaagtt gaaaggcacc gggcacgtgc
      781 ttccatgaat catgatcata aatcctttca actggatacg attccttgat gatgcgaatc
      841 atgtcctctg cgaattcatc cgcaaacccg aacttctcga tttcgacctt catggccttc
      901 atgatttctt cgagatagat ggtgtcgcga tgcgcgtatt ccatttgcga agcagtgagc
      961 ggtcttttct tccaattgga tttctgttct gttttttcta gatcgcggcc tgtgtaatgc
     1021 ttcacgagag acacaaggga gcagctgaca aaaccgagca tcctgcaacc caccatgata
     1081 tcgaatagat tctcgaactt gaatttgcga agacgccgca actctgaaat atcagaggcg
     1141 gccgtgtgca ttacttttac aatggaaggg tttgcgaaga tctctgcgag accgtccact
     1201 gtgagattgg ccagaggatc gatgagataa ttcttaccgg aagcgctgat ctgaatcaaa
     1261 cagagttcag actcatatgt atagaagcta gaactctccg tgtccacaga aacgtactgt
     1321 gacctgccca ttacagagac tgccagatcc agatcctgct gtttatcaat gaactggtag
     1381 cccacaatgc cagagtactg atgagcggtc ctctgtcaaa ttttgattgg taaacaaaca
     1441 agccacacga tccttgcaca tggcccttcg caacagagac cttgtgccag aaaagcgcgc
     1501 tcctgtaaaa aaatcagtga tcgtggcggg tctagtggca gcggccattc tggccttaat
     1561 gctgcggctc gtcgtgcatg ctgtgtggat ttttcctctt cgcgtgggca cggccagcat
     1621 ggagcctgcc gtaaaaaaag gacaaacagt ctacgttgtg aagcgcgtag aactgcaatt
     1681 tggcgatatc atattgtaca aacacccgga taacccggag gttcggctcc tgggaagaat
     1741 cacaggtcta cccggagatg caatagaaat gaaagaaaag caactcttga gaaacggtgc
     1801 accagtccag gatgcctggc aaatacgcac tcagacgggc tattctttac ctggttccgt
     1861 atttccacgc gactcctttg cagaaagaaa gatcactacc ggcaaatact ttgtgttatg
     1921 cgacctgcgc acggagtgcc tggactcaag acttcttgga gaaatcagcc gcgagatgat
     1981 cgtcggcaga attcgaccag attgactcct tacagaatcg cagccatctg cgaaggatcg
     2041 gagaaaaatc cattcagagt ttcccccgaa tatttttcga tcaggagttc cgcataagcc
     2101 tccgtatgcg gacgaaacgc ttgtgacccg gcagagtcct ggatcatttc tgatagagaa
     2161 aaagcagcgt acaagagatc gcagaattca gttcgatgag aatccaccca gaaatcagac
     2221 tgcttttcgc aatcattgat ccgcgaagaa atgagtgcct gcgcgcgcgc tgcaagagga
     2281 agagatccta cgttgcgcgc ggcctctgaa agcatttctt taaacgcatt gtaaacattt
     2341 ggtctgccat acgccttgag agcgtgttcg caaagaagag gatgcgttcc ttcccaggtt
     2401 tcattgatga tagcgtcatt gtgcagacgc ggcaaaatag aaaaatcgcc caggatcccg
     2461 ttgcctccga gaaccagaat gccttcgtga gtcagatgag tggccagaac agtggaggca
     2521 tacttcagga gaggagtcag catctttgag agcacatgat ccttcgcgtt gagatcgtaa
     2581 ttgcgcataa cggcaaactc aacgacagtc tgcagcacct gcatgcgcgc gagagtgcga
     2641 aggactgctg gaagttcaat gatcttcttc ccatacgctg tccgtttttg agtgtaagcg
     2701 cgcacttcca gataagatct tctcccatga cccaca
//
LOCUS       DAORFF010000536         2725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_26039_length_2725_cov_3.349064, whole genome shotgun sequence.
ACCESSION   DAORFF010000536 DAORFF010000000
VERSION     DAORFF010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26039_length_2725_cov_3.349064"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /gene="prfB"
                     /locus_tag="PKK76_16975"
     CDS             <1..1115
                     /gene="prfB"
                     /locus_tag="PKK76_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNL70226.1"
                     /translation="AETAKQFQERWDQKGITGILDELEDLESLTARADFWDDPERAKK
                     TSMKKSELEKRTQPWRDLRRDLDEFPDLVDLTLEEVKDQKAAIEGLEGDLHKIRERLE
                     DTLLAEALTGEDDGRDAIVTISSGAGGTESQDWTEMLLRMYLRWFEKRGFRVETADIQ
                     QGEEAGIKNATLIVRGENAFGLLNAENGVHRLVRISPFDSNKRRHTSFAAVHVTPDID
                     DSIIVNIDEKDLRIDTYRSSGAGGQHVNKTDSAVRITHNPTGIVVQCQNERSQMKNKS
                     TAMKMLKARLHELEKRKLEDDIESRSGEKRDVAWGSQIRSYVMHPYKLVKDLRTDYET
                     GNVDAVMDGDLDPFVNAYLKSLAGMGPALKAGEKAE"
     gene            1205..>2725
                     /locus_tag="PKK76_16980"
     CDS             1205..>2725
                     /locus_tag="PKK76_16980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNL70227.1"
                     /translation="MSDFSKSVFKYIAGIAVTIFLCVFLVFWLIYSRTLQAQEEELSR
                     IVAQEARALSVNGTLIRTATGRDTTPEEIQRLLAVTKEGTGFEIVVGRDVGDRFSIFA
                     SSERGFESLDLPWTSDRAVPMKRALKGETGHDSTVDYEGKEVIAAYAPVPDLHLGLVA
                     KMDRSVIRELYFYSVILSILLAAALSILVSVIVWRVSRISLEKRILRPTLSRYELMLN
                     RSPHCIHEMGPNGAFISMNDAGLRMIRKRKEEIEGLHCLDLVHHSDQARVKMLFERAL
                     KGEPVEFEFKMVSGKSARSDFTPWKNEAGKVTRIIGTTVDLSDELQAAENVRTLENLL
                     METERISQTGSWSLDLKTGELVWSREIYHIFEIEPEFEPSYEEFVNSIHPEDREFVNT
                     AYRESVSSRQQFDVTHRLLMTDGRTKYVREHGITYYDGEGAAVRSVGTVQDITARIAA
                     ETKLAQSEAKYRLLFESSPDAIQVVDPVKLTRLEFNDVSAARLGYTREEYASIKVGN"
BASE COUNT          761 a          644 c          702 g          618 t
ORIGIN      
        1 atgcagaaac cgcaaagcaa tttcaggagc gctgggacca gaaagggatc acaggcatcc
       61 tcgatgaatt agaagatctg gagtctctaa cggcacgtgc cgacttctgg gatgacccgg
      121 agcgtgcgaa gaagacttcg atgaagaagt cagagcttga aaaaagaacg caaccctggc
      181 gcgatcttcg ccgcgatctg gatgaattcc cggacctcgt agacctcact ctggaagaag
      241 tcaaagatca aaaagcggcc atcgaaggac tcgaaggtga tcttcacaag atcagggaac
      301 gcctcgaaga cacgctcctc gcagaagcgt tgaccggcga agatgacggc agagacgcca
      361 ttgttacgat ttcatccgga gccggcggca cggaaagcca ggactggact gaaatgcttt
      421 tgcgcatgta ccttcgctgg tttgaaaagc gtggctttcg cgtagagaca gcagacatac
      481 aacagggtga agaagcggga atcaaaaacg ccacgctcat tgttcgcggt gaaaatgcat
      541 ttggcctttt gaatgcggaa aatggagttc acagactggt tcggatttcc ccttttgatt
      601 caaacaaaag aagacatact tctttcgcag ccgtgcacgt gactccggac attgatgatt
      661 ccatcattgt caacattgat gaaaaagatc ttcgcattga tacatatcgt tcgagcggcg
      721 caggtggaca gcacgtaaac aaaacagact ccgctgtgag aatcacacac aatcctacag
      781 gaattgttgt gcagtgtcag aatgaacgtt cgcagatgaa aaacaaatct actgcgatga
      841 aaatgctcaa ggcaaggctc cacgaattag aaaaacgaaa actcgaagac gacattgaaa
      901 gtcgctccgg agaaaaacga gacgtagcat ggggcagtca aattcgaagc tatgtaatgc
      961 atccctacaa actggtgaag gaccttcgca cagattatga aacaggcaat gtagacgcag
     1021 tcatggacgg agatctagat ccgttcgtaa atgcgtatct caaaagcctc gctggaatgg
     1081 gaccggcgct caaagccgga gagaaagcag agtaaaggca tccgtagtgg tacggatatt
     1141 tttttccaat cacggattcg ttttctactc gtaagacccg agcgtttcgc cttcgtttta
     1201 cacgttgagc gatttttcta aatcagtttt caaatacatc gccggaattg cggtaaccat
     1261 ttttctctgt gttttcctgg tattctggct gatctattcc cgtacgctgc aagcgcagga
     1321 agaagagctg tctcgaattg ttgcacagga agcacgagcc ctgtccgtca acggtacctt
     1381 gatccgtact gccactggac gcgacactac acctgaggaa atccaaagat tactcgccgt
     1441 gacgaaagag ggaactggat ttgaaattgt ggtcggtcgc gatgtgggcg atcgcttcag
     1501 catcttcgca tcgagcgaac gaggttttga atctctcgat ctcccctgga caagcgacag
     1561 ggccgttcct atgaagcgag cactgaaagg tgaaactggt catgattcca ccgtggacta
     1621 tgaaggcaaa gaggtgatcg cagcctatgc acctgtgcca gaccttcatc tgggtctggt
     1681 tgccaagatg gatcgcagtg tgattcgcga actgtatttc tattctgtca tcctgagcat
     1741 cttgctggct gcagcgcttt ctattcttgt gagtgtcatt gtttggcgcg tttcgcgcat
     1801 ttcgttagaa aaacggattc tacgacccac tctctcgcgc tatgaactca tgctgaatcg
     1861 gtctcctcat tgcattcacg agatgggccc caacggcgcg tttatttcca tgaacgatgc
     1921 cgggctacga atgattcgaa agcgaaaaga agaaattgag ggacttcatt gtcttgatct
     1981 ggttcaccat tccgatcaag cgagggtcaa gatgctcttc gaacgcgctc tcaaaggtga
     2041 acctgtcgaa ttcgaattca aaatggtttc aggaaaaagc gcacgctcag acttcactcc
     2101 gtggaaaaat gaagcgggca aagtcacgcg catcattgga actactgttg atttgtcgga
     2161 tgaacttcag gccgccgaaa acgtgcggac gcttgagaat ctgttgatgg aaacagaacg
     2221 catcagccag acgggaagct ggtccctgga cctgaagaca ggcgaactcg tctggtccag
     2281 ggagatttac cacatctttg aaatcgagcc cgaatttgaa ccatcttacg aagaattcgt
     2341 aaacagcatt catccagaag atagagagtt cgtaaacacg gcgtacaggg aatcagtttc
     2401 gtcacgtcag caatttgatg tcactcacag gctcctcatg acggatggcc gcaccaagta
     2461 tgtaagagaa cacggaatta cgtactatga tggagaggga gccgcagtgc gatctgttgg
     2521 gactgtgcaa gacatcacag caaggattgc cgcagagaca aaactcgcgc agagcgaagc
     2581 aaagtacaga ctgctttttg agtcatctcc agatgctatc caggtggttg atccagtcaa
     2641 gctcacgcga ttagagttca acgatgtgtc cgcagcacgc ctcggctaca cccgcgaaga
     2701 atatgcgtcc atcaaagtgg gcaat
//
LOCUS       DAORFF010000537         2724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_26064_length_2724_cov_3.295616, whole genome shotgun sequence.
ACCESSION   DAORFF010000537 DAORFF010000000
VERSION     DAORFF010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26064_length_2724_cov_3.295616"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..870
                     /locus_tag="PKK76_16985"
     CDS             145..870
                     /locus_tag="PKK76_16985"
                     /inference="COORDINATES: protein motif:HMM:NF036494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70228.1"
                     /translation="MGLVTERPAFLVTRTAVQRTEERAVMQGDVILSTPGGRPSSDGK
                     IKDEYYGDLPLPLAIVLKNGRFTDENAVSIQVLRDRVERMIRLPVRNASEDLSVVPEK
                     LFHSSYLITGGLVLLELTGERVRAMDDSTGRFRYLRSRYWGGDNMIGERIIFVDRVLP
                     VKQNEGQRPQRAVLTSVNGIGVRNLLHAHSIIREARRKGEDVVFTFDGNEIAVFSAAA
                     LAQADEQVRNSYGIPFLYGESLE"
     gene            854..1321
                     /gene="smpB"
                     /locus_tag="PKK76_16990"
     CDS             854..1321
                     /gene="smpB"
                     /locus_tag="PKK76_16990"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNL70229.1"
                     /translation="MSPSNKKPPPSFENKQARRKYEVTETYEAGIALTGTEVKSLRHG
                     KCEILDAHIIIKEGELFVLNLRIEPYEHGGSYNHAEKRTRKLLMHRTEISKIGAKIRE
                     KQMTCIPLKVYFNEKGKVKVLIGLGKGKKMADRREDEKKAVAKREMERAIRGR"
     gene            1323..2648
                     /gene="der"
                     /locus_tag="PKK76_16995"
     CDS             1323..2648
                     /gene="der"
                     /locus_tag="PKK76_16995"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNL70230.1"
                     /translation="MSANKKLPLIAIAGKRNAGKSTLLNIMFGSKRAITDATPGLTRD
                     ILQVEIKRGKHRFLLCDTPGLDLDGSEPLDANILSRTREFLEGVDLILFVMEPPGPTP
                     FDLSFLDFFRKQRDLSRILFVLNKIDNPKQDSAEAEFYQMGFPELICISALGRKNLGT
                     LYDQIEKRLPAPGSTVASAGPPPDLTLCIVGKPNAGKSSLLNRLSGQELALVSDIPGT
                     TRDSIDSLFTYYGKTIRLIDTAGLKKTGKLKDSVEFYSMTRTKRAIAEAEIVVHVLDA
                     TLGVTDYDKKIVSLIEENGKPVVFAVNKWDAVEEKNQKAFMDRMYFMFPHAQSYPAIF
                     ISAKTGTRIGKVLEEAMKLKERLSFRVGTAQLNQWLKEWNRRLTNTQKTAKIYYGTQA
                     GTAPPQFIFFVNKKDHFRKDALLYFENQIRKDCDLHGIPITIQLREKES"
BASE COUNT          742 a          666 c          705 g          611 t
ORIGIN      
        1 tgatcctgcg gcgcttgtac cggccctgcg cgcgcgcatt caagctgtgg acagccgcac
       61 cgccgtcacg cgcgttcgca ctttcgacga actggtttcg gcctcggtgg cgcaaccgcg
      121 tctgagcctg cgtatcttcg ttacatgggc ctggtaacag aaagacctgc ttttctggta
      181 acacgcacag cggtacagcg aactgaagaa cgcgcggtga tgcaaggaga cgtgattctt
      241 tctacgccag gtggaaggcc ttcctcagac gggaagatca aagacgaata ctacggtgat
      301 ttgccacttc ctctggcgat cgtgctgaaa aacggccgat ttacggatga aaacgctgtc
      361 agcattcagg ttcttcgcga tcgggtagag cgcatgattc gcctacctgt gcgaaacgca
      421 tccgaagatc tgtctgttgt tcccgagaag ctatttcatt cttcgtattt gattaccggt
      481 ggactggtgc tcctggaact cacgggagag cgggttcgtg ccatggatga ctctacgggc
      541 cgatttcgct acctgagatc gcgctactgg ggcggggaca acatgatagg agaacgaatc
      601 atattcgtgg accgggttct gcccgtgaaa cagaatgagg gccagaggcc gcagagggca
      661 gtgctgacgt ctgtcaacgg aatcggggtc agaaacctgc tccatgctca ttcgatcatc
      721 cgggaagcgc gccggaaagg cgaagatgtt gttttcacct ttgacgggaa tgaaatagct
      781 gtatttagcg cagctgctct ggctcaggca gacgagcagg ttcgcaattc atacggtata
      841 ccatttttgt acggtgagtc cctcgaataa aaaaccgcct ccctctttcg agaacaagca
      901 ggcacgccgc aaatacgaag tcacggagac atacgaggca gggatagctt taaccgggac
      961 agaagtgaag agtcttcgtc acggaaagtg cgaaatcctg gacgcgcaca tcatcatcaa
     1021 agaaggggaa ctttttgttc tgaaccttcg aattgagcct tatgagcacg gcggatccta
     1081 caatcacgca gagaaacgta cgcgcaaact gctgatgcat cgaactgaga tctcaaagat
     1141 tggcgcaaag atcagagaga agcagatgac atgcattcct ttgaaagtct acttcaatga
     1201 aaaaggcaaa gtgaaagttc tcatcggtct gggcaaagga aagaaaatgg ccgatagacg
     1261 cgaagatgaa aagaaggcag ttgccaaaag agaaatggag cgcgccataa gaggccgttg
     1321 atatgagcgc gaacaagaaa ctccctctca tcgccatagc tggaaaacgg aacgcgggaa
     1381 aatctactct actcaatatc atgttcggat ccaagagagc gatcacggac gcaactcctg
     1441 gcctcacgcg tgacatctta caggtagaaa tcaaaagagg gaagcatcgc ttccttttgt
     1501 gcgacactcc agggcttgac ctggacggca gcgaaccgct cgacgcaaac atactctcga
     1561 ggacccgaga atttctagaa ggagtggacc ttatcctctt cgtgatggaa ccaccgggtc
     1621 ccactccatt tgatctttca tttctggatt tttttagaaa gcagcgtgat ctatccagaa
     1681 ttctatttgt tctcaacaag atagacaatc caaaacagga ctcagcagaa gctgagtttt
     1741 accagatggg tttcccagag ctcatttgca tttccgccct cggaagaaag aatcttggga
     1801 ctctgtatga tcagattgaa aagagactgc ccgcaccggg aagtacagtc gcatctgccg
     1861 gcccgcctcc agacctgaca ctttgcattg ttggaaaacc aaacgcgggc aaatcgtctt
     1921 tgctcaatcg cctttctggc caggaactgg cacttgtttc agacattccg ggaaccacgc
     1981 gcgacagcat agactctctg tttacgtact acggaaaaac aattcgcctc attgatactg
     2041 caggtctaaa gaagacaggc aagctaaagg attccgtgga attctattcc atgactcgca
     2101 caaagcgagc gattgcagag gcggaaatcg tagtgcatgt gctcgatgca acgctcggag
     2161 ttacggacta cgacaagaag atcgtaagcc tgattgaaga aaatggaaaa ccggttgttt
     2221 ttgctgtgaa taaatgggac gctgtggaag agaaaaacca gaaggcgttc atggacagaa
     2281 tgtatttcat gtttccccat gcccaatcct accccgcgat attcatatct gcgaaaacag
     2341 gcacgaggat tggaaaggtt ctagaagaag ccatgaaact caaggaacgc ctgtcctttc
     2401 gcgtgggcac agcacagctc aatcagtggc taaaggaatg gaaccgtcgc ctgaccaaca
     2461 cccagaagac tgcaaaaatc tattatggaa cgcaggcggg aacggcccct cctcaattca
     2521 tcttcttcgt aaataagaag gatcattttc gtaaagacgc acttttgtat ttcgagaacc
     2581 agattcgaaa agactgtgat ctccatggca ttccgatcac gattcagctc agggagaaag
     2641 aaagttgatc tgggtgcagg ttctactcat tccgttttcg tttctgtgcg gctccattcc
     2701 ttttgcagtg atcgtgggcc gcat
//
LOCUS       DAORFF010000538         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_26077_length_2723_cov_3.597826, whole genome shotgun sequence.
ACCESSION   DAORFF010000538 DAORFF010000000
VERSION     DAORFF010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26077_length_2723_cov_3.597826"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1541)
                     /locus_tag="PKK76_17000"
     CDS             complement(48..1541)
                     /locus_tag="PKK76_17000"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNL70231.1"
                     /translation="MIFSSALFFFFFAAVFTMHWAILPGLVPPRHLRTARHAALLIAS
                     YVFFASYEWIYAFLLLFFTLTAYGSAFYLQPGGSRDKLRGWLLLAVIVTFVGLLGYYK
                     YYNFFLGNLLTLVGIVGYHPDVVFHTIALPLGISFFTFQALSYVIDVWRGKTPVEQSF
                     LRFALFKSFFPQLVAGPIVTAREFLPQLYADKELDLERLRSGARWFALGFIKKAVIAD
                     NVSPAVDMLFANPAGQFLHNSSGAWLAALGFSAQLYADFSGYSDMAWGSAIWLGFDLP
                     ENFRMPYMARSITDHWRRWHMSLARWIREYVYISMGGNRVSFVRHKVNLLFSFVISGL
                     WHGANWTYVVWGTAQGIILVIENTLGEWKKKREEGQPVTEPGAFTRIISVPVQLTLWL
                     YMFIFTTSSHVVFRSPSLSAAWTHLKTMYSFSSQGLAPQPSIYRPILISTVVVLLGQL
                     FGYWYFEKKKFQVKVPIWLEFAFYPFMILALIQLAAPEGGAFIYFVF"
     gene            complement(1538..2197)
                     /locus_tag="PKK76_17005"
     CDS             complement(1538..2197)
                     /locus_tag="PKK76_17005"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNL70232.1"
                     /translation="MHVVSRMKSDLSSAFLFCGIYLGVLFITSSLIPLNAWTAALPGA
                     LSTTVCLILAALASRIMPAQMGSFRPLSAAFIGLFLFAAQSRMTEPLPGFLREPVDLP
                     GAFFSMVLLGPLCEEAFFRGLLLDRFGGNARAVVLQAVFFAVSHFSLAGLPFAFTGGL
                     LLGFIAISTRGILSAFVAHATLNAIVISQRILWPETIPLWIIALCFACGLFGIWRFRK
                     A"
     gene            2196..2417
                     /locus_tag="PKK76_17010"
     CDS             2196..2417
                     /locus_tag="PKK76_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HNL70233.1"
                     /translation="MIQLHRLNKKAFFLNHRQIEIMEANPHTVITLANENKYIVEESI
                     SDIIALIRDYERQIVADPGSGIHSRTLSP"
     gene            2514..>2723
                     /locus_tag="PKK76_17015"
     CDS             2514..>2723
                     /locus_tag="PKK76_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoflagellar basal body-associated protein"
                     /protein_id="HNL70234.1"
                     /translation="MAGEDVMDEEEGGSGEAPAEAAGSPASGLGKVVKILIYVALGAV
                     GLVIMAVIAYYVASFAAARQYKEVAS"
BASE COUNT          775 a          663 c          722 g          563 t
ORIGIN      
        1 ttggccgagc tgtatgaatt tttctttgac gaaagtgcga aaaaatttta aaacacaaag
       61 tatataaacg ccccgccttc cggagctgca agctgtatga gagcaaggat cataaacgga
      121 tagaacgcga attcaagcca gatgggaact ttgacctgga atttcttttt ctcgaagtac
      181 cagtagccaa agagctgtcc aagcagaacc acgacggttg aaatcagaat cggacgatag
      241 atcgatggct gtggcgccag cccctgactg gaaaaagaat acattgtctt gagatgagtc
      301 catgcagcag aaaggctcgg gcttctaaac actacgtggc tgctggttgt aaaaataaac
      361 atgtagagcc acaacgttag ttgcacaggc acagatatga ttcttgtaaa ggcgccgggt
      421 tctgtgactg gctgcccttc ttcgcgtttc ttcttccatt cgcccagtgt gttttcaatg
      481 accagaatga ttccctgggc tgttccccac actacatacg tccagttcgc tccatgccag
      541 aggccgctga tcacgaaaga gaaaagaaga ttcaccttgt gacgtacaaa cgaaacgcgg
      601 tttccaccca tcgaaatgta gacgtattcg cggatccatc tggcaagaga catgtgccat
      661 cgtctccagt ggtccgtgat ggatctggcc atgtatggca tgcgaaagtt ttcgggcaga
      721 tcaaatccaa gccatatggc cgatccccag gccatgtcgc tgtatcctga gaagtctgcg
      781 tagagctggg cagaaaatcc aagtgcagca agccaggcac cgctcgaatt atggaggaac
      841 tgtcccgcag gatttgcgaa gagcatgtcc acggcaggcg aaacgttatc tgcaatcact
      901 gccttcttaa taaacccaag tgcaaaccat cgcgcgccgg agcgaagtct ttctagatcc
      961 aattccttat ccgcatagag ttgcggtaga aattcgcgag cagtgacgat tggtccggcc
     1021 accagctgag ggaagaatga cttaaacagc gcgaatctga gaaatgactg ctccaccgga
     1081 gttttaccgc gccaaacatc gatcacataa ctcagtgcct ggaatgtgaa aaatgaaatg
     1141 cccaggggaa gagcaattgt atggaaaacc acatccgggt gatagcccac aatcccaaca
     1201 agagtcagta gattgcccag gaaaaagtta tagtatttat agtatccgag cagtccaacg
     1261 aacgtgacaa tcactgccag aagaagccat cctctcagtt tgtcgcgcga accacccggc
     1321 tggagataaa aagcagaacc gtaggcagtc agagtgaaga acaagagtaa gaatgcgtaa
     1381 atccattcat aggatgcaaa aaacacatac gacgcgatca gcaatgccgc atgacgggct
     1441 gtgcgaagat ggcgtggtgg aacgagtcca ggcaggatcg cccagtgcat ggtgaaaact
     1501 gcggcaaaaa agaaaaagaa aagtgcgctt gagaagatca tgcttttcta aatcgccata
     1561 tgccaaacag gccgcacgca aagcagagcg ctatgatcca gagaggtatg gtttctggcc
     1621 acagtatgcg ctgcgagatt acaattgcgt tgagagtagc gtgagccacg aaagcggata
     1681 ggatcccgcg cgtggaaatc gcaatgaaac ccagaagcag accgccggtg aacgcgaaag
     1741 ggagtcctgc caggctgaaa tgactcacgg cgaagaacac tgcctgtagc accaccgctc
     1801 tggcatttcc cccgaaccga tccagtagca aacctctgaa aaaagcctct tcgcataggg
     1861 ggcctaacag gaccatactg aagaaagctc ccgggaggtc tactggttcg cgcaagaacc
     1921 ctggcagcgg ttctgtcatt ctgctctggg ccgcaaacag aaaaaggccg atgaatgccg
     1981 cactgagagg acgaaaactg cccatttgtg ccggcatgat gcgtgaagca agtgcggcga
     2041 ggatcaggca gactgtggtg gaaagagctc ctgggagcgc ggccgtccag gcattgagtg
     2101 ggatgaggct cgaggtaatg aacagaacac ccaggtaaat gccgcagaaa aggaatgcgc
     2161 tcgacaaatc tgatttcatg cgggaaacaa catgcatgat ccagcttcac aggctgaaca
     2221 agaaagcttt ttttctgaac catcgccaga ttgaaatcat ggaggcgaat ccgcatacgg
     2281 tcatcactct ggccaatgag aacaagtata ttgtagaaga atccatttct gatatcattg
     2341 ccctgattcg cgattacgaa cgccagatcg tcgcagaccc tggttccgga attcactcgc
     2401 gtactctttc gccctgaacg ggctgctttc gctgtaaaaa agaagcgatg cctctgccaa
     2461 attgaccgat tataaatcag gactgcccgt gggcggagca gtcggaggat tgcatggccg
     2521 gtgaagatgt catggacgaa gaggaagggg ggagtggcga agctcccgct gaagccgctg
     2581 gctcgcctgc aagcggtcta ggtaaagttg taaaaatcct gatctatgtt gccctgggcg
     2641 cagtaggtct ggtcattatg gcagtcatcg cgtactatgt tgcctctttt gccgcagccc
     2701 gtcagtacaa agaggttgca tcc
//
LOCUS       DAORFF010000539         2716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_26194_length_2716_cov_3.606539, whole genome shotgun sequence.
ACCESSION   DAORFF010000539 DAORFF010000000
VERSION     DAORFF010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26194_length_2716_cov_3.606539"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKK76_17020"
     CDS             <1..393
                     /locus_tag="PKK76_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNL70235.1"
                     /translation="KLGQYLDPISDKTVTLVAMLDCALHYGFPWFVFGFCVFREILGV
                     WVGSYLYFKRDMQGSPNMWGKFGVGAVALSVIWHVLTPYFKLKGLTGIWLDAWISAAL
                     IFFIFLAGMIAYGRTYWRIILHGKTKST"
     gene            500..1645
                     /gene="recA"
                     /locus_tag="PKK76_17025"
     CDS             500..1645
                     /gene="recA"
                     /locus_tag="PKK76_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004766281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNL70236.1"
                     /translation="MATKARKLEVVETRRPDRNAPNPQLEERKKAVVGAIQQIEKQFG
                     KGSIMKLGDESAFQEVGTIPTGALTLDMALGVGGLPRGRIVEIFGPESSGKTTVCLST
                     VAHAQKAGGIAAYVDAEHALDPQFARKLGVNIDDLLVAQPDNGEEALEIAESLVRSNA
                     VDIVVVDSVAALVPKAELEGNMGDAQMGLHARLMSQAMRKLTGTISRSNTTVIFVNQI
                     RNKIGVMFGSPETTTGGNALKFYASIRLDIRRTETLKKGDEAYGNRVRVKVVKNKMAP
                     PFRQAEFDILFDSGISREGCLLDLGTSLGILERTGTWYSYKGDRVGQGRENTCQYLRE
                     NPKTADQVEQEIRQKHKEKDSLLKKEAAGKPPEMEIDEETGEIIPPE"
     gene            1642..2658
                     /gene="argC"
                     /locus_tag="PKK76_17030"
     CDS             1642..2658
                     /gene="argC"
                     /locus_tag="PKK76_17030"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNL70237.1"
                     /translation="MTEPIARKAAVLGAAGFTGKELLRILAAHPLFEVSHVTSDKHVG
                     KTLVETFPDLPRPKKNLTFQLHSDPIPQDAFVFLATPNEVSLERVPELRKKGHRVVDL
                     SGAFRLHDPAQFKEFYKLDHNAMDTMKECVYGIPELYRQKIRTANLIANPGCFPTGVI
                     FPLSILQEFAEDMVGVSIDAKSGVSGAGGRVEDGGFSYSSVYENFRAYKILGHQHWPE
                     IKEYSAAGKLADKIVFTPHLLPLFRGILSTITIFWKKMPELAEKFAHACEGEPFVRLL
                     SAPEDVQLARIQHTNYIDVGIRSRGNVTVIVTAIDNLVKGAAGQAIQNLNLMAGLDET
                     LGLV"
BASE COUNT          728 a          627 c          711 g          650 t
ORIGIN      
        1 aaactaggcc agtatctgga tcccatctca gacaagacgg tgactctggt ggccatgctc
       61 gactgcgccc tccactatgg attcccgtgg tttgtttttg ggttctgcgt atttcgggaa
      121 atactcggtg tctgggtcgg atcctacctc tatttcaaga gagatatgca gggaagcccc
      181 aatatgtggg gtaaatttgg agtgggagcc gtggcccttt ctgtgatctg gcacgttctg
      241 actccgtact tcaaattgaa gggtcttact ggaatctggc tcgacgcatg gatttctgct
      301 gctcttattt tcttcatatt cctggcagga atgatcgcct atgggcgaac ctactggcga
      361 attatccttc acggtaaaac taaatctact tgacaaatgt tacctagttc tggtatggct
      421 ttttatgtca caccggtcaa ataggatggc aaaaaagatt ggctgcaact tcctggaaga
      481 gcagcccgag gagatgtaaa tggctacgaa ggcaaggaaa ctggaagtgg tagagaccag
      541 aagaccggac cgcaatgctc ccaatcctca gttagaggaa cgcaaaaagg cggtggtggg
      601 agccatccag caaattgaaa agcaatttgg gaaaggctcc atcatgaaac tgggagatga
      661 gagtgctttt caagaagtgg gaaccatccc cacaggagcg ctcactctgg atatggctct
      721 tggagtcggc ggacttccgc gcggtcgcat tgtagaaatc tttggacctg aatcatccgg
      781 caagacaact gtttgtcttt ccacagtcgc gcatgcacag aaggcggggg gcattgcagc
      841 ctacgtggat gcagagcatg cactggatcc gcagttcgcg cgaaagctag gagtcaacat
      901 tgatgacctg ctggtcgcgc aaccggacaa tggagaagag gctcttgaaa ttgctgagtc
      961 tctagtgcgg tccaatgccg tggatattgt ggtcgtggat tcagtggcag cactcgttcc
     1021 aaaagcagaa ctggaaggaa acatgggaga tgcgcaaatg ggtcttcatg caagactcat
     1081 gagccaggcc atgcgaaagc tcacaggcac tatttcaaga tccaacacta ctgtgatctt
     1141 cgtgaatcag attcgcaaca agatcggtgt gatgttcggt tctcctgaaa caaccacagg
     1201 tggaaacgca ttgaagttct atgcttctat ccgtctggac attcgccgca ctgaaactct
     1261 caagaaggga gatgaggcat acggaaatcg tgtgagagtc aaagtagtga aaaacaaaat
     1321 ggctcctccc ttccgccagg cagaatttga tattctgttt gattcaggaa tcagccgaga
     1381 aggctgcctt ctggatctgg gcacttcact tggtatactg gaaagaaccg gaacctggta
     1441 ttcctacaaa ggggaccgag taggccaggg tcgcgagaat acttgccagt atctgcgcga
     1501 aaatccaaaa accgccgatc aggtggaaca ggaaattcgc cagaaacata aagaaaaaga
     1561 cagcctctta aaaaaagagg ctgctggtaa acctccagaa atggagatcg acgaagaaac
     1621 gggagagatt attccgcctg aatgactgag ccgatagcca gaaaagctgc cgtattgggc
     1681 gcggcggggt ttacggggaa agaacttctt cgcattctcg ccgcgcaccc tctttttgaa
     1741 gtttcccatg tcacttctga caagcatgtc gggaaaacac tggtagagac attccctgat
     1801 ttacccagac caaagaaaaa tctaacgttc cagttgcaca gcgatccgat tccccaggat
     1861 gcttttgtat ttctggccac tcccaacgag gtttctctag aaagagtccc tgagttacga
     1921 aaaaagggtc atcgcgtagt cgacttgagc ggtgcgtttc gtttgcacga tccagcacag
     1981 ttcaaggaat tttacaaact ggatcacaac gcaatggaca cgatgaagga atgcgtgtat
     2041 gggattccgg aactctatcg ccagaagatt cgcactgcca acctgattgc aaaccccggt
     2101 tgctttccaa ctggagtgat cttccctctt tctatcctcc aggaatttgc agaggatatg
     2161 gttggagttt ctattgatgc gaaatctgga gtttctggcg ccggagggcg cgtagaagat
     2221 ggaggatttt cttattcatc cgtttacgaa aactttcgcg catacaagat tctagggcac
     2281 cagcactggc cagaaataaa agaatactca gccgcaggaa agcttgcgga caaaatcgtt
     2341 tttactcccc atctcttgcc tctctttcgg ggaattttat ccacgatcac aatcttctgg
     2401 aagaaaatgc ctgaactcgc agaaaagttc gcacatgcgt gcgaaggtga gccatttgtt
     2461 cgccttttgt ctgcgccaga agatgtacag ctcgcgcgaa tccagcacac aaactacatc
     2521 gacgttggaa ttagatcacg cggaaatgtc accgtgattg taaccgcgat cgacaatctg
     2581 gtaaagggtg ctgcgggtca ggccatacag aatttgaatt tgatggcggg tctggatgaa
     2641 acgctcggtt tagtatagaa cttgacgcac gaccctggca ccgttttgct gaatgaacgg
     2701 gatgtagcgc aggggt
//
LOCUS       DAORFF010000540         2716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_26200_length_2716_cov_3.093950, whole genome shotgun sequence.
ACCESSION   DAORFF010000540 DAORFF010000000
VERSION     DAORFF010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26200_length_2716_cov_3.093950"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..174)
                     /locus_tag="PKK76_17035"
                     /pseudo
     CDS             complement(1..174)
                     /locus_tag="PKK76_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
     gene            155..1195
                     /locus_tag="PKK76_17040"
     CDS             155..1195
                     /locus_tag="PKK76_17040"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNL70238.1"
                     /translation="MTGNASDPARHPDDIQKYLSKEQHRLYRMIWENFVASQMADEVA
                     DQTTAEIAGDGLIFRANGRKVKFPGFTEIQSPDEKKKKEKETELPALEVGQNALLEKT
                     LPEQHFTQPPPRFTDASMVKTLEESGVGRPSTYAPTIMTLLKRYYVVRKQRALQPTEL
                     GKLVNSVMAVHFRELVDSGFTARMEDDLDRIAGSELAWQDMLRTFYSPFVDILKNATE
                     QIGEMRNVLDEVTEYVCEKCGKQMVKRIGRNGYFLACPGFPECKNAKPIPLGKCPKCA
                     DGLVVQKATRRGRPFFSCNRYPECDFSTWDKPTGELCPNCGKLLLEKSSREKGKYIAC
                     CACDFEEAEKSA"
     gene            1237..2589
                     /locus_tag="PKK76_17045"
     CDS             1237..2589
                     /locus_tag="PKK76_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HNL70239.1"
                     /translation="MLRFGLAAAFALSFLSSPLFAWSEHAMLGYYGLITMLEIKNAQP
                     VPAESLESFLQKEEAGLEKVLADIEEWNKKNLVVYPVPTPEIAFKRTGIDHKRRFLEA
                     LRMDPESRTPLYLQLLPGQEAGGRPAFPVKEISVFKNPMWIAQTRFTTIREGEAVLPL
                     EVVASACDEPDYGFDIGIWQDNNSPHSKKFNMGIQPFGNPNLEYGSQAPVHMGFYHEA
                     GIIYLLAGFLKRTYPEMRVHQAYALSRFAFRTGHPYWGFRFMGWGLHYVQDLTQPYHA
                     RVLPGVGTLRMLWINFMDIIGRHEGKKNIVQLVSNRHLALENFQRRIMTRDLRNKDLN
                     QPYWFALQNTEFDSKYNHFNKKYLRDVLTEESVSKSDELSREIMALMPVRLVDDPGVE
                     FEEEKGSMDLLRTMETNQPGSVSQFANVLARLTGAAGVHSRNYVRAILATQDTYDSVN
                     "
BASE COUNT          725 a          646 c          692 g          653 t
ORIGIN      
        1 gccgatgcag gcggccgcgt caaaggcttc gtccgacttc gccttctcga tcggaatcgc
       61 gttggcgtcg ggagcattgc cggtattggc ggagatataa ccgccggcgg cgatgatctt
      121 atcgaaggcg gtgcggtcga ccaccaggtc cttgatgacc gggaatgcct cggatcccgc
      181 gcgtcatccg gatgatattc agaaatatct ttccaaagaa caacatcgtc tttatagaat
      241 gatctgggaa aactttgtgg cctctcagat ggcggatgaa gtggcagatc aaaccactgc
      301 agagattgca ggagacgggc tcatatttcg cgcgaacggt cgcaaagtga agtttccggg
      361 ctttacagag attcaatctc cagatgaaaa aaagaaaaag gaaaaggaaa cggaactgcc
      421 cgcactggaa gtgggacaga atgctctact tgaaaaaact cttcctgagc aacactttac
      481 tcaaccgcct ccgcgtttca cggatgcatc tatggtgaaa actctagaag aaagcggagt
      541 gggtagacca tctacgtacg cgcccaccat catgactttg ctcaagagat actatgttgt
      601 gcgaaagcag cgtgcacttc agccaacgga acttggcaag ctggtcaaca gtgtgatggc
      661 agttcatttt cgcgagctgg tggattcagg atttaccgcg cgcatggaag atgatctcga
      721 tagaattgca ggttcagagc tcgcctggca ggacatgctt cgaacgtttt attctccttt
      781 cgtggacata ttgaaaaatg caaccgagca gattggcgaa atgcgcaacg tgctcgacga
      841 agttacagag tatgtctgcg aaaaatgcgg caaacaaatg gtcaaaagga ttggcagaaa
      901 cggctacttc cttgcctgtc ctggctttcc tgagtgtaag aacgcaaagc caatcccact
      961 cggaaaatgt ccaaaatgtg cagacggact cgttgtgcag aaggcaacca ggagaggtcg
     1021 gccgttcttc agttgcaatc gctatccgga gtgcgatttc tcaacatggg ataagccaac
     1081 cggagaactc tgtccgaatt gcggtaagct tcttctggag aaatcatcgc gcgagaaagg
     1141 caaatacatt gcctgttgcg cctgtgactt tgaagaggca gagaagtcgg cctgaaaagt
     1201 aattgtaata tatataacaa actatcaacc tggagcatgc ttcgttttgg gttggccgcc
     1261 gcttttgcac tctcattcct atcttctccg ctcttcgcct ggagcgaaca tgcaatgctt
     1321 ggctattacg gcctcattac catgctcgag atcaagaacg cacaacctgt tccggcagag
     1381 agcctggagt cctttctaca gaaggaagag gcgggccttg aaaaggtgct cgcagacata
     1441 gaagagtgga acaagaagaa cctcgtcgtt tatccggtgc ccacacctga gatcgcattc
     1501 aagagaacgg gcatagatca taagagaaga tttctggaag cgcttcgcat ggatccggaa
     1561 tctcgcactc ctctgtatct acagctattg cccggccagg aagcaggagg tcgtcccgca
     1621 tttccagtca aagaaattag cgtatttaag aatccaatgt ggattgctca gaccagattc
     1681 acaaccatac gcgaaggaga agcagttctt cctcttgagg tggttgcgtc tgcatgcgat
     1741 gaacccgatt atggatttga tattggaatc tggcaggaca acaattcacc tcacagcaag
     1801 aaattcaaca tgggcattca gccttttgga aatcccaacc tcgaatacgg aagtcaggct
     1861 ccggttcata tggggtttta tcacgaggca ggcatcatct atcttctcgc cggattttta
     1921 aagcgcacgt atcctgaaat gcgcgttcac caggcgtatg ctctttctcg ttttgcattc
     1981 agaacgggtc atccatactg gggctttcgc tttatgggct ggggtttgca ctacgtgcag
     2041 gatctcactc agccatatca cgcacgagtc cttcctggcg tgggaactct caggatgctc
     2101 tggatcaatt tcatggacat cattggaagg cacgaaggta agaaaaacat agtgcagctt
     2161 gtatccaatc gccatcttgc cctggaaaat ttccagagaa gaatcatgac tcgcgatttg
     2221 aggaacaaag atctaaatca accttactgg tttgcgttgc aaaacactga gttcgattca
     2281 aagtacaatc attttaacaa gaaatatttg agggatgtcc tcacagaaga atccgtgagt
     2341 aagtcagatg aactcagccg cgaaatcatg gcactcatgc cagttcgtct cgtagatgac
     2401 ccaggtgtag aatttgaaga agaaaaggga tccatggact tgcttcgcac catggaaaca
     2461 aatcagcctg gatctgtaag tcagttcgca aatgtactgg ccaggctgac aggtgcggcc
     2521 ggggttcatt cgcgtaacta cgtgcgcgcg attcttgcca cccaggatac gtacgattct
     2581 gttaattgac tgcggcttgc gctggctgta gagtttcttt gaagaatctc acggcagccg
     2641 ccacttcgga tccattgtaa gtaagagtag tgcgcatgtg tccgtagggc agttctctaa
     2701 attccacctg tacccc
//
LOCUS       DAORFF010000541         2706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_26349_length_2706_cov_4.043380, whole genome shotgun sequence.
ACCESSION   DAORFF010000541 DAORFF010000000
VERSION     DAORFF010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26349_length_2706_cov_4.043380"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PKK76_17050"
     CDS             complement(<1..519)
                     /locus_tag="PKK76_17050"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL70240.1"
                     /translation="MRKILWTLLLAWLQLLACSAPAQCKIGDSSCALNSLPLLFSRTI
                     LARFLFSANQTGNNLNVYRISQATGQLTSVGTIAAGTYPTSVGSALDSRFVYVSNQNS
                     NDITQLIVDPISGAPTANGSLTIAGGSPQAILIPTGGQFGFSANYALNNLSNLVINSM
                     TGQLTQSSVTGAG"
     gene            601..939
                     /locus_tag="PKK76_17055"
     CDS             601..939
                     /locus_tag="PKK76_17055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL70241.1"
                     /translation="MPLQTNQIGPNLIVYLDGNIDLSQSVLIETELLKLIEQHSSKNL
                     ILDFTQVSAMSSSGVRVLVLLRRTLKETKQKLALCNLGAPVLRIIKLLELDGRFEIFN
                     SQEDALRAHE"
     gene            1012..1482
                     /locus_tag="PKK76_17060"
     CDS             1012..1482
                     /locus_tag="PKK76_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70242.1"
                     /translation="MIKTLVVEKNFRGSMRDVFYDLAENPNYFPKYFKGFAPFVPGIR
                     KITPQWTGQIVPGGVRKVDLSDGSFVMERVTVHEPPRLQKYEMAELNSMQKLVCQKMH
                     AEYSLEDKGEFVHVKWTYWIETEKLLQKPLVAIVGWAFHKAMHRFMDAASSHAR"
     gene            1517..1948
                     /locus_tag="PKK76_17065"
     CDS             1517..1948
                     /locus_tag="PKK76_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70243.1"
                     /translation="MEKTYLPFLIVAIVPLFWLILVTGIMYLISFTGWRNLAKKYANQ
                     GENPQTGFHWASVQTSWIGRYNNCINLAITPRGLFVQPWLLFRFAHPGLLMPWNMLKH
                     ARPRSVLFWDFAELEFDDGKDSLLVYAPSRILQAPGFPRQT"
     gene            1960..2295
                     /locus_tag="PKK76_17070"
     CDS             1960..2295
                     /locus_tag="PKK76_17070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL70244.1"
                     /translation="MQIQSKEQDRYMLHRIEGEVDLYNSGVLKSALMNAFAANGKNIL
                     LDLSAMVYIDSTGMSVLFQTATQLREAKKDLKVFGATPAVMSVLSMVNVPGKLQICQT
                     EEEAAMFFR"
     gene            complement(2297..>2706)
                     /locus_tag="PKK76_17075"
     CDS             complement(2297..>2706)
                     /locus_tag="PKK76_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL70245.1"
                     /translation="KIKTIGDAYMIVGGLPEPDPHHAENVARFALEMLDVVRQYRQEN
                     GLPLELRIGINSGDAVAGVIGKKKFIYDLWGDSVNTASRMESHGLPGQIQVTESTYML
                     LKDKFQFSERGSMEVKGLGIVKSYLLMEAMEPI"
BASE COUNT          682 a          660 c          666 g          698 t
ORIGIN      
        1 acccgctcct gtaacagagg actgcgtgag ctgtccagtc atagaattta tgaccagatt
       61 ggacagattg ttcagcgcat agttggctga gaatccaaac tgaccacccg ttggaatgag
      121 gatggcctga ggagaaccgc cggcgatcgt tagactacca ttggccgtgg gtgctccgct
      181 tatggggtca acaatcaact gggttatgtc gtttgagttc tggttgctca cgtaaacgaa
      241 cctgctgtcc agggcagaac caacggacgt tggataagtg cctgcagcaa tcgtcccgac
      301 actggtcaat tggcctgtgg cctggctgat tctgtagacg ttcaggttgt ttccggtctg
      361 gtttgcagaa aacagaaatc gtgccaggat ggtgcgtgaa aacagcagcg gaagggaatt
      421 cagcgcgcag gaggagtccc cgattttgca ctgagccggt gccgagcagg cgaggagttg
      481 aagccaggcc agaaggagtg tccagaggat ttttctcacc tattattaac agttttgtgc
      541 gccttccctt gcaaaaaaaa tttcgcatga cggagatgcc gcggccggcc gaaatattcc
      601 atgcccttgc agacgaatca gatcggaccc aacttgattg tttacctgga cggaaatatc
      661 gacctctccc agtccgttct cattgaaacc gaactactga agctcattga gcaacactcc
      721 agcaagaacc tcatcctgga tttcacccag gtgagtgcca tgtcctcctc tggcgtacgc
      781 gttcttgtgc ttttgcggcg aactctcaaa gaaacaaaac agaagcttgc actttgcaac
      841 ctgggcgcgc ccgtgctgcg tattatcaaa ctcctcgaat tagatggacg gttcgaaatc
      901 tttaactccc aggaagacgc tctgcgtgct catgaatgaa atgcatgccg acactttcgc
      961 agaacgacag gtttgttcta tgcagttttc acgtcccggg catcttgagc tatgattaag
     1021 acactcgtcg tagaaaagaa ttttcgtggc agtatgcgcg atgttttcta tgatctcgca
     1081 gagaacccga actactttcc gaaatatttc aaagggtttg ccccttttgt tccaggaatt
     1141 cgaaagatca cgcctcaatg gactggacaa attgtaccgg gcggggtccg aaaagtcgac
     1201 ttgagcgacg gttctttcgt gatggagcgt gtcacagtcc atgaacctcc caggttgcaa
     1261 aaatacgaaa tggcggaact caattccatg caaaaactgg tttgccagaa gatgcatgca
     1321 gagtattccc tggaagacaa aggcgaattt gttcatgtga aatggaccta ctggattgaa
     1381 acagaaaagc ttcttcaaaa accgttagtc gccattgtcg gttgggcctt ccacaaagca
     1441 atgcacaggt tcatggacgc agcatcatcg catgccagat aggctgccta aaaggaaaac
     1501 aagaccggag taacaaatgg aaaagacata cttaccgttt ttgatcgtag ccattgtacc
     1561 tttgttctgg ctcattcttg taaccgggat catgtatctg atatcgttta ccggatggcg
     1621 taatttggcc aaaaagtatg ccaatcaggg tgaaaatcca cagaccggtt ttcactgggc
     1681 cagcgtgcaa acgtcctgga ttggcagata caacaactgc atcaatctgg ccatcactcc
     1741 gcggggtttg tttgtgcaac cctggctgct ctttcggttt gctcatccag gattgctgat
     1801 gccctggaac atgctcaagc atgctcgccc tcgctctgtg cttttctggg atttcgcaga
     1861 acttgaattt gatgatggca aagattcctt gctggtgtat gcaccttctc ggatcttaca
     1921 ggcgccagga tttcccaggc aaacgtgaag gaatagcaca tgcagattca aagcaaagaa
     1981 caggatcgat acatgctgca ccgcatcgaa ggtgaggtgg atctctataa ttctggggtc
     2041 cttaagagtg cactgatgaa tgcatttgcg gctaacggca aaaacatttt gctcgattta
     2101 tctgcgatgg tgtacattga ttccaccgga atgtccgttt tgttccagac tgccactcaa
     2161 ttgcgcgaag caaagaagga cctcaaagtt tttggcgcga caccggccgt gatgagcgtg
     2221 ctgagcatgg tgaatgttcc aggcaagctt caaatctgcc agacagaaga agaagcggcc
     2281 atgttctttc gctaacctat atcggttcca ttgcttccat tagcaaatag ctcttcacaa
     2341 tgccaagtcc cttgacttcc atggatcctc tttcggaaaa ctgaaatttg tctttcagta
     2401 gcatgtacgt agattccgtg acctgaatct ggcccggaag tccgtgcgat tccatgcgag
     2461 aagcagtgtt tacgctatct ccccatagat cataaatgaa tttctttttt ccaattacgc
     2521 cggccaccgc atctccggaa ttgattccaa tgcggagttc tagaggcaga ccattctctt
     2581 gtctgtactg gcgcactaca tcgagcattt ccagggcaaa gcgtgctacg ttttcagcat
     2641 gatgcggatc gggttctgga agccctccca ctatcatgta agcatccccg atcgttttga
     2701 ttttct
//
LOCUS       DAORFF010000542         2694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_26546_length_2694_cov_3.250853, whole genome shotgun sequence.
ACCESSION   DAORFF010000542 DAORFF010000000
VERSION     DAORFF010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26546_length_2694_cov_3.250853"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PKK76_17080"
     CDS             complement(<1..555)
                     /locus_tag="PKK76_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70246.1"
                     /translation="MQIDFFEEFPSEETLSKAKLMNGEFLVYIASSTISDFHRHRDML
                     SRISSNAKAAYWPLLRRSYWVSPFSFPEELEGLYRDLENSSPMDVLIDLELPLLRPAL
                     FLKNVPHFLENKKLIRKILGLGHRFLAAVYPATGILSESILRALGIWHPQTESCVMYY
                     TSMIQKPAIIRWMQSAIRRRALLGH"
     gene            complement(562..1443)
                     /locus_tag="PKK76_17085"
     CDS             complement(562..1443)
                     /locus_tag="PKK76_17085"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNL70247.1"
                     /translation="MSDFENTLPEAALRRHDHIERLKSLPEARKGVFAGFTGTPGAGK
                     STLIGALCLRLIEMDPHIRVAVVAVDPSSEVSGGALLGDRTRVRFPVDEDRLFFRSQA
                     SDRQLGGMSPGTFQVCRLLYHLFDLVIIETVGIGQNEIEVQRLADRVYLVLQPLAGDQ
                     IQFLKAGIMEIPDVFLLNKSDEVEAARKTYHALKASIQFTRPGDGDIQILQTSAVMGT
                     GIDEVCADLMELVRKPRDGESLMREKEERYFSQWVREEYGRSGIKKLLDLGGAAFWIK
                     ECGSYDAATRRLIDKFN"
     gene            complement(1467..>2694)
                     /locus_tag="PKK76_17090"
     CDS             complement(1467..>2694)
                     /locus_tag="PKK76_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNL70248.1"
                     /translation="KYRMFRYGVQVNSLGLTEAQPENNAWRILIEALGVTMSKRARCR
                     ALQLPAWNEALSHPRPWDQQWALRLQQILAYETDLLEYPDLFDGSKVVESKVEALKEE
                     CRAELDKILGMGGVRAAVESGYLKTSLVKSMSERLSKINSGELTVVGINKWKDGIASP
                     LTSGNDGGIFKVDPAKVKETMDNLEATKRKRDPAKVEAALAKLKAAAKSGENMMEPSI
                     ECALARVTTGEWSDALRSVFGEYRPATGIDGQKLLLEDERVASVRKRVETFTAKRGHR
                     PRIVVGKPGLDGHSNGAEMIAVTARHSGFDVIYSGIRLTPEEIVQSAVEENADIIGIS
                     ILSGSHIELAEQIVAKLKEMKAERIPVVFGGIIPKEDFAVLEKTGIRKLFTPSDYKLM
                     DIMDSILNVVEEESAK"
BASE COUNT          602 a          703 c          636 g          753 t
ORIGIN      
        1 atgtcccaat agggcgcgcc ttcggatcgc agattgcatc cagcggatga tggccggctt
       61 ttggatcatg ctggtgtaat acatcacaca gctttctgtc tgaggatgcc agatgccgag
      121 tgcgcgcagg atggattctg atagaattcc agtggcgggg taaactgcag ccaggaagcg
      181 atgccccagt ccgaggatct tccggatgag ttttttgttt tccaggaaat gaggcacgtt
      241 tttgagaaag agggcgggcc gaagaagagg taattccaga tcgatcagta cgtccatggg
      301 cgagctgttt tccagatcgc ggtacaggcc ttccagttct tctggaaaag agaaaggaga
      361 aacccagtaa gatcgacgga gcaagggcca gtaggcagcc ttcgcattcg aagaaattct
      421 agagagcatg tcgcggtggc gatgaaaatc agaaatggta gagcttgcga tgtaaactag
      481 aaactcacca ttcatgagct ttgccttaga cagcgtttct tcagacggaa attcttcgaa
      541 gaaatcgatt tgcatcttgc ttcagttgaa cttgtcaatc agtctgcgag tggctgcgtc
      601 atagcttcca cattctttga tccagaaggc tgcacctccg aggtccagga gttttttgat
      661 tccacttctt ccgtattctt cgcggaccca ctgggaaaag tatctttctt ctttttcgcg
      721 catcaaagat tcaccgtccc ggggctttcg caccagttcc atcagatccg cgcacacttc
      781 gtcaatgccc gtccccataa cggcactcgt ctgtagaatc tgaatgtctc catccccggg
      841 cctcgtgaac tgaatgcttg ctttgagggc gtgataggtc tttctggccg cttccacttc
      901 atcgcttttg tttaagagaa aaacatccgg aatctccatg attccagcct tcaaaaactg
      961 aatttgatct ccagctaacg gttgtaagac gagatagact cgatctgcga gtctctgcac
     1021 ctctatttcg ttttgaccaa ttcctacggt ctcaatgatt acaagatcaa acagatgata
     1081 aagaagcctg catacctgaa acgtgcccgg gctcattcca ccgagttgtc tgtcagaggc
     1141 ctggctgcga aagaaaagtc tgtcctcatc tacaggaaat cgaacacgag ttctatctcc
     1201 cagaagcgct ccgcctgaaa cttcactcga aggatctaca gccacgacag ccactctgat
     1261 gtgcggatcc atctctatga gtctgaggca aagcgcaccg atgagagtgg actttccggc
     1321 gcctggagtg ccagtgaaac ctgcgaatac acctttgcgc gcttctggaa gcgatttcag
     1381 cctttcgata tgatcgtgcc ggcgcagtgc cgcttcgggc agcgtatttt caaaatcaga
     1441 gatcagccgt gcaagagcat acttctctat ttcgcagact cctcttctac cacattcaaa
     1501 atggaatcca tgatatccat gagcttgtag tcagaaggag taaagagttt gcgaattcct
     1561 gtcttttcca gaacagcgaa atcttctttt ggaatgattc ctccgaatac caccggaatt
     1621 cgctctgctt tcatttcctt cagttttgca actatctgtt ctgccagctc aatatgcgac
     1681 ccgctcagaa tggaaatgcc aatgatgtcc gcgttctctt ctacggcgct ctgcacgatt
     1741 tcttcgggag tcagcctgat tccggaatag attacatcaa aaccagaatg ccgcgctgtc
     1801 actgcaatca tctctgctcc gttggaatga ccatcgagtc ccggctttcc gactacgatg
     1861 cgcggtctgt gacctctttt tgcagtgaat gtttctactc tctttctcac gcttgcaaca
     1921 cgttcatctt ctagaagtag cttctgcccg tcgattcctg tcgcgggtct gtattctcca
     1981 aaaacactgc gaagtgcgtc tgaccattcg ccggtggtta cgcgagcaag agcgcattca
     2041 atggatggct ccatcatgtt ttctccgctc tttgcggcag ctttgagttt ggcgagtgct
     2101 gcttctactt ttgccgggtc gcgtttgcgt ttggtggctt ccaggttgtc catggtctct
     2161 ttgacttttg ctggatctac tttgaagatg ccgccatcat ttccgcttgt gagaggactt
     2221 gcaatgccgt ccttccattt gttgattcca actacagtca gttcgcctga attgattttg
     2281 ctcagtctct ctgacatgga tttgaccaga gacgttttga ggtaaccgct ttctacggcc
     2341 gcgcgcactc cgcccattcc cagaatctta tccagttccg ccctgcattc ttctttcagc
     2401 gcttctactt ttgattctac taccttgctt ccgtcaaaca gatccggata ttcgagcagg
     2461 tctgtttcgt atgcgagaat ttgctggagt cttaaggccc attgctggtc ccacggacga
     2521 ggatgcgaca gcgcttcatt ccacgcaggg agttgaagag cacggcatcg tgcacgttta
     2581 ctcatggtta caccaagagc ttctatgaga attctccacg cgttgttttc cggctgcgct
     2641 tctgtgagcc cgagggaatt tacctgcacg ccgtaacgaa acattctata tttt
//
LOCUS       DAORFF010000543         2690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_26614_length_2690_cov_3.375332, whole genome shotgun sequence.
ACCESSION   DAORFF010000543 DAORFF010000000
VERSION     DAORFF010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26614_length_2690_cov_3.375332"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PKK76_17095"
     CDS             <1..971
                     /locus_tag="PKK76_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70249.1"
                     /translation="LLSLGALAKILTGPLAGGPPLIGMSILILSGSILVITQIRPPKF
                     LLLDDAKGALIICEKTMDQPTALIPYSAFEEIRTARGLPSRNRRRTFGVDLVKKDGVR
                     YAIFETRTLQAAEEATALLKKNIDLRSQDNASRIVSSTFRRENKNDAIKIEWRMPVRV
                     LRTILSAAMATGFSILLIESYDSVPVFLFWPLQIFLCAIAVLSLASLFYPRKQVVTID
                     RIQLKAEVTGIFAKQLIVPLSEIASVQFQFAAAWEDTIYLLNEHETAVMRAYQEGTLD
                     PQRALEIGKMMLQARRIPSGGLTASEMISLEQILEETLTDQGRAVQ"
     gene            968..1711
                     /locus_tag="PKK76_17100"
     CDS             968..1711
                     /locus_tag="PKK76_17100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL70250.1"
                     /translation="MSFKDHFSNHSRIYSEFRPDYPDAFIEWVCSLPEKKNLAWDCGT
                     GSGQAAVQLKKHFNRVIATDPSEEQIKHAKQLEGIEYRVLPAEKTNLDTASMDLICVA
                     QALHWFDFDLFFAEARRVLKPRGILCAWSYGLLETEEPVQTIIESFYRETVGPYWPAE
                     RRHVDERYATIPFPFYKIEAPYFQIERTWDMDSYLGYVSTWSAVQRMRKATGIDPLIE
                     LRNQLRPFYPGPLLIRWPIFALTGRAGNA"
     gene            complement(1890..2336)
                     /locus_tag="PKK76_17105"
     CDS             complement(1890..2336)
                     /locus_tag="PKK76_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70251.1"
                     /translation="MKKILIIAVAAFVVSCSKPAPAPETWNCDFKGQYVEKGETVPFT
                     WKVTWANTQGDNWKITGAAAEADAKSSTTGTCDAKTCKIDETYTAGENKGKSFYWVGT
                     YTDAETKNENVLITSFTGTFGPTDADRTSMGTWSAKADCKNANPAK"
     gene            complement(2440..>2690)
                     /locus_tag="PKK76_17110"
     CDS             complement(2440..>2690)
                     /locus_tag="PKK76_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70252.1"
                     /translation="GIPTVVWYPPVNPQFRQTIYERHGKELDPAGFDRIVRESGLAVA
                     DSTSFEKKCNRWEDAVHPSRRCNGLIAMAILNAAAAQR"
BASE COUNT          653 a          689 c          667 g          681 t
ORIGIN      
        1 ttctgttatc tctgggcgcc ctcgcgaaaa tactgaccgg acccttagca ggcggacctc
       61 cattgattgg aatgagcatc ctcatcctct cggggagcat cctggtcatt acccagatcc
      121 gcccgccaaa attcctccta ttggacgatg caaaaggggc tttgattata tgcgaaaaga
      181 caatggatca gccaactgca ctcattccgt actccgcttt cgaggaaatc aggaccgcca
      241 gaggcctgcc ttcgcgcaac cgcaggcgaa cgtttggagt ggatctggtc aaaaaagacg
      301 gggttcgcta tgccattttt gaaacccgga cgctacaggc ggccgaagaa gccactgccc
      361 ttttgaagaa gaacattgac ttgcgctctc aagacaacgc ttcgcgaatc gtatcgagca
      421 cattcagaag ggagaacaag aatgatgcca tcaaaattga atggcgtatg cctgtgcgcg
      481 tgttgcgcac gatcctttct gcggcaatgg caaccgggtt ttcgatactg ctgatagaat
      541 catacgactc ggtcccggta tttctcttct ggcctttgca aatctttctg tgcgcgatcg
      601 ccgtgctttc cctggcctct ctcttttatc cacgcaaaca ggttgtcaca atagatcgca
      661 ttcaactcaa agcggaggtc actggtatct ttgcaaagca actcattgtt cccctgagcg
      721 aaattgcgtc cgttcaattc caatttgcgg ccgcctggga ggatacgatt tacctgctaa
      781 atgagcatga aactgcggtt atgcgagcgt atcaagaagg aactcttgat ccgcagcgcg
      841 ccctcgaaat cggaaagatg atgctgcagg ccaggagaat cccctctgga ggattgacgg
      901 catcagaaat gatatcactc gaacaaattc tggaagaaac cctcacggat cagggtcgcg
      961 cggtccaatg agtttcaagg atcacttttc aaaccattct agaatctata gtgaatttcg
     1021 ccccgactac ccggatgcgt tcatcgaatg ggtctgcagt ctgcctgaaa aaaagaatct
     1081 ggcctgggac tgcgggacgg gttccggaca ggccgcggtt caactcaaga aacacttcaa
     1141 tcgtgtaatc gccacggatc caagcgaaga gcaaatcaaa catgctaaac aactagaggg
     1201 gatcgagtat cgcgtcctcc ccgcagaaaa gacaaacctg gatactgctt ctatggactt
     1261 aatctgcgta gctcaagccc ttcactggtt tgattttgat ctgttcttcg cagaagcgag
     1321 acgagtgctc aagccgagag gcatcctttg cgcgtggagc tacggtctac tagaaacaga
     1381 agaaccagtt caaaccatca ttgaatcatt ctacagagag accgtgggac cctactggcc
     1441 ggcggagcga cgccacgtag acgaacgtta tgcgacgatt ccatttccat tttataagat
     1501 cgaagcccca tactttcaaa tagagaggac gtgggacatg gattcatacc tgggctacgt
     1561 ttctacctgg tctgcggttc aaaggatgcg gaaagcaact ggaatagatc cgctgataga
     1621 actcagaaat caattgcgtc cattctaccc cggtcccctg ctcattcgct ggccaatttt
     1681 tgctctcacg ggtcgcgcag gaaatgcttg atagacagta agggctttgc gtgcgatttt
     1741 ttttcaacca ttctagccag ggctgatgcg gagaatgatc tggagaagaa agtagattgc
     1801 tccagccttt gcggccagaa agtttaaaaa acgaggcgcc acttctggcg cccacattgc
     1861 gtgccgaatg gcacctattc gatttttgat tatttagcag gatttgcgtt tttgcaatct
     1921 gcttttgcac tccaggtacc catgctggtt ctgtcagcat ctgttggtcc gaatgtgcct
     1981 gtgaagctgg tgatcagcac gttttcgttc ttagtttcag cgtctgtgta agtgcctacc
     2041 cagtagaacg acttgccttt gttttcgccg gcagtgtacg tttcgtcaat cttgcaggtt
     2101 ttcgcatcgc aagttccagt tgtgctggat ttcgcgtctg cttctgctgc cgcaccagta
     2161 atcttccagt tgtcgccctg tgtgtttgcc caggttactt tccatgtgaa tggaacagtt
     2221 tcgcctttct caacatactg acctttgaag tcacagttcc aggtctctgg agctggagcg
     2281 ggcttggagc agcttactac gaacgcagca actgcaatga tcaaaatctt cttcatcgta
     2341 ctctcctgaa tttttccgtc cggtttaaga acggacgtca aggttccggc aacgggtgac
     2401 tctgagcaac tgaaaaatca agtcaatctg atgacgaaac taccgctgtg cggcggcagc
     2461 gttcagaatg gccatggcaa tgagcccatt gcagcgacgc gaaggatgca ctgcatcttc
     2521 ccagcgattg catttctttt caaaggaagt cgaatctgcg acagccagac cggattcccg
     2581 gacaatgcga tcaaatccgg ccgggtcgag ttccttgccg tgtctttcgt agatggtctg
     2641 tcgaaactgt ggatttacag gtggatacca cactacggtc ggaattccgg
//
LOCUS       DAORFF010000544         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_26964_length_2670_cov_3.286807, whole genome shotgun sequence.
ACCESSION   DAORFF010000544 DAORFF010000000
VERSION     DAORFF010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26964_length_2670_cov_3.286807"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..312)
                     /locus_tag="PKK76_17115"
     CDS             complement(100..312)
                     /locus_tag="PKK76_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70253.1"
                     /translation="MLREIVAARQNNQASHQLSNSALSIERNPPKSKPLTELNQFQQP
                     DTDRRNAGIDTSGPSSSDVYKVNMQA"
     gene            complement(398..>2670)
                     /locus_tag="PKK76_17120"
     CDS             complement(398..>2670)
                     /locus_tag="PKK76_17120"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNL70254.1"
                     /translation="HNYRLEAHDEALRVARNANDMFRLIYQGLRTIARLPGVRKIQDQ
                     NLVFRGGGAGFEADAKLAVQELYNNLATNVAISEVYIAPRDLDPDAPAYSNAHKEPLV
                     TFDELIVGQTALGNKGDNAESVPEIELYEYRLMKKQIEYFRAKTPRIGMIQELDFPAR
                     SGQEVVTCDNSHYNPKKPDDKDRSGFVYSVPFFDASGEMGGIVVAVMLTSAAADSLPS
                     GNFALRNTTYNYTIEHAKPGETRSSHAFLEKGEANPDLLYSEVLKLDIRDDEAPWMLW
                     AGLPNEMFTSRTEVRDARVFVTGALGFIWILASAAVLLILSTRRNRAILETQNETLET
                     LVGERTADLNESRGQMRDILQNVSQGILTISRDRTVNSEYSGSIPQILGLDPSGKPFV
                     ELFGEPLLKERVSKFIHALFDNPFMGADMFDSANPLRSIVYFTPERKVAHTLSFGFAR
                     IWKDKAVDKILVIIDDRTREAELERAVKDREKEQSSRVEKLYQILNLEPEVFRSFVTE
                     SLESLGFIRRRVEETLQGGKAEETLRGALRDLHTVKGNARALNLDSIAQRAHAVEEDI
                     GKMVEGSAFEPSQLEAILLEIDSVVEEISDGENLFGRILGMKDLIQVRTVDALAELEE
                     RSHRLIEKEALDSGKSANLEFHSNGTRLRSEILMRVRNALTHLLRNAIAHGIEGHEER
                     RAAGKTLDGRISVEIKEVEDQILVVCEDDGRGIDPEELARAAVSKEAGMAGVNAYIEK
                     PVRGNVLWDKIAPFIQ"
BASE COUNT          589 a          728 c          656 g          697 t
ORIGIN      
        1 ctgggagaag aaaggtcaga aggcgtgctg gcaaaagccc gcaaagatgc gattcgcctt
       61 ctactgaggg cctggcggcc ctatttcgag tgagtcagat caggcttgca tattcacttt
      121 atacacgtct gaactggaag gcccggacgt atcaatcccc gcattccttc tgtccgtgtc
      181 tggttgctga aactgattga gctcagtgag aggtttgctt ttgggcggat ttctctcaat
      241 agacagagcg ctgttggaca gctggtgact tgcctggttg ttctgacgag cggccacaat
      301 ttcgcgaagc atgactcagg atagcaccca atctgaacca ctgcaagcgc ttttctgtga
      361 aaaaccaaaa gccccggggt ggttcccgga gaggatgcta ctgaatgaag ggagcgattt
      421 tgtcccagag aacgtttccg cgcaccggct tttcaatgta tgcattgact ccagccatgc
      481 ccgcttcttt cgagacagcc gcgcgcgcca gctcttctgg atcaattcca cgcccatcgt
      541 cttcgcatac gacgagtatc tggtcctcta cttctttgat ctccacagag atccggccgt
      601 ccagggtttt gcctgctgct cgtcgttctt cgtgaccttc gattccatgc gcgatcgcat
      661 ttcgtagcag gtgcgtcagc gcatttcgca cgcgcatgag gatctcactg cgaaggcgag
      721 tgccgttaga atgaaattcc aggtttgccg acttaccaga atcgagagcc tctttttcta
      781 tcagcctgtg gcttctctct tcgagctccg caagcgcgtc cactgtgcgg acctggatga
      841 gatctttcat tcccagaatt ctcccaaaca gattttctcc atcggagatt tcttccacca
      901 cagagtcgat ttcaagaagg atcgcctcca gctgcgacgg ttcaaacgcg gacccttcta
      961 ccatcttgcc gatatcctct tcgaccgcgt gggctctctg cgcgatcgag tccagattca
     1021 gagcccgcgc atttcccttc acagtatgaa ggtctcgcag tgccccgcgc aaagtctcct
     1081 ctgcttttcc gccctggaga gtctcctcca cgcgcctgcg aatgaatccc agactttcga
     1141 ggctttccgt cacgaagctc cgaaatacct ccggctctag attcagtatt tgatacagct
     1201 tctccacccg tgagctctgt tctttttccc ggtccttgac tgcgcgctcc agctccgctt
     1261 cgcgcgttct atcatctatg atgaccagaa tcttatccac agccttatct ttccatatcc
     1321 gtgcgaaacc aaacgaaagt gtatgcgcga cctttctctc gggagtgaaa tacactatgc
     1381 ttcgcagagg attggcagag tcgaacatgt ctgctcccat aaatggatta tcaaagagag
     1441 catgaatgaa tttagaaacg cgttctttta gaagtggttc gccaaagagt tctacgaacg
     1501 gttttccgga aggatcaaga cccaggattt gcgggatgga tccagaatat tctgagttca
     1561 cggttctgtc gcgggatata gtgagaatcc cctggctcac attctgcaaa atgtctcgca
     1621 tctggccgcg gctttcattg agatctgccg tccgttcgcc tactagagtt tcgagcgttt
     1681 cattttgagt ctcaaggatg gcgcggttac gcctcgtgga taaaatcaag agaactgcgg
     1741 cagaggcaag aatccagatg aaaccaagtg cgcctgtgac aaaaactcgt gcgtcacgca
     1801 cttccgttcg cgatgtaaac atttcgttgg gaagcccggc ccacaacatc cagggtgcct
     1861 catcgtcccg gatatcgagt ttcaaaacct ccgagtaaag cagatccggg tttgcttctc
     1921 ccttttcaag aaacgcatgg gaagatctgg tctctccagg tttcgcatgt tcgattgtat
     1981 agttataggt agtattgcgc agagcgaaat ttccggatgg aagtgaatct gcagcagcag
     2041 atgtcagcat cacggccacc acaattcccc ccatctcccc gcttgcatca aagaaaggaa
     2101 ctgaatacac aaaaccggat cggtccttgt catctggctt cttcggatta taatgggagt
     2161 tgtcgcatgt gaccacttcc tgtccagatc gagcgggaaa gtccagttcc tggatcatgc
     2221 cgatgcgcgg agtctttgcc cggaagtatt caatctgttt tttcatcaaa cggtattcat
     2281 acagctcaat ctcaggaacg ctttctgcat tgtcgccttt gttgccgagt gcagtctgtc
     2341 ctacgatgag ctcatcaaaa gtcaccagag gctctttgtg tgcattactg taagcaggtg
     2401 cgtccgggtc tagatccctg ggagcgatgt atacttctga aatggcaacg tttgtggcga
     2461 ggttgttgta gagctcctga acggccagct tcgcatccgc ttcaaaacct gccccaccac
     2521 ctcgaaacac caggttctgg tcctggatct ttcgcacacc tggaagacgt gcaattgtgc
     2581 gaagcccctg gtagatgagt cggaacatgt cattcgcatt tcgcgccacg cgaagagctt
     2641 cgtcgtgtgc ttcgagtcga taattgtgca
//
LOCUS       DAORFF010000545         2669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_26982_length_2669_cov_3.376052, whole genome shotgun sequence.
ACCESSION   DAORFF010000545 DAORFF010000000
VERSION     DAORFF010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26982_length_2669_cov_3.376052"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PKK76_17125"
     CDS             <1..974
                     /locus_tag="PKK76_17125"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNL70255.1"
                     /translation="AMLADGLGLFGIEALQVDATSASGAAALRAAWLSVASGASDFAL
                     AVGVEKMGSTEDATPILARGLNQEEEKGETMVTIVSRLMDLYFKNAMRSGVSEEAMRE
                     GFAHFAVNSHQNGMRNPDAFLKKELSLESAMNARVIQGPLRLSDCSPVCDGSAAVLLC
                     ASGMAGSFSDHPVQLSASSCATDRFTVGRRRDPLVLSASFHSVEKAYRQAGITPRDID
                     FFEVHDAFSIMAVLSLEACGFAERGKGWRAGTEGTIYPGGKLPLSTFGGLKSRGHPVG
                     ATALYQACEIVRQLRGQAGPNQVEGKTALMQSIGGAGTTILTHIFQR"
     gene            981..>2669
                     /locus_tag="PKK76_17130"
     CDS             981..>2669
                     /locus_tag="PKK76_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70256.1"
                     /translation="MSDIENVPEESFTPILSPAEGRMIPVEPEEDLISDIDLRNLMVQ
                     LSPMHLKDVNAMEMAVENFYVFHDSPEALSHPESLQYLGARDLEWLASKHAIRIFQYI
                     TCEGEQKRLSVHRVVFRPSNLKAEVLIRIKRACIRTSAWAIIRHLQFREHVLSRMETS
                     GEISVWIRKRLLGEFAGSPMRKLSESQVKRLPQIFERLAFEHFLDQDIKPLKELKQGI
                     FYLLTRKPEANGFLEESYPPPADIAEYLRNRMQRPILTQLSDEFHVLTPAIPAPGIAG
                     RSELDYIHDLYCEACRAILIVGMDEDAQRAEYADPMELIHIISAHASFYDEEHWPRGR
                     EFLSELKGIIELLVLSTRMRREMILGGVAQKLISSLNNRFDPLLLDAHDLVSSMPDDA
                     RTLFPDTGAAAAALMSILKSNAELAFFTERRGSGPHEQSFVLFRANLPGAFVQNPERR
                     TFFHAVAKENGYPRGIYDFLISVKSSDPKKLVDDQLELSRAIREWEDHTQKVQKPKSV
                     GWIERLTNWILKLLGFGEFGRRKSDKSKNTTDASGAKAKHAAIPARAQKAIDYVE"
BASE COUNT          683 a          665 c          701 g          620 t
ORIGIN      
        1 gcgccatgct ggcagatggt ctgggtctct ttggaattga agcacttcaa gtggacgcaa
       61 cgtccgcgtc cggtgccgca gctctgcgcg cggcatggtt gtctgtcgca tccggtgctt
      121 ctgatttcgc actcgccgtt ggcgttgaga agatgggttc cacagaagac gcaacgccca
      181 ttctcgcgcg tggcctcaac caggaagaag agaaaggaga gacgatggtc accatcgtct
      241 cacggctcat ggatctatat tttaagaatg cgatgcgctc aggcgtgagc gaagaagcca
      301 tgcgcgaagg cttcgctcat ttcgcagtca actcgcatca aaatggaatg cgcaatccag
      361 atgcctttct aaagaaagaa ttgtctcttg aatccgcgat gaatgcgaga gtgatccagg
      421 gtcctcttcg tctgagcgat tgttcccctg tgtgcgatgg atctgctgcg gttcttctgt
      481 gcgcatctgg aatggcgggt tccttttccg atcacccggt ccagctatcg gcttcgtcct
      541 gtgccacgga tcgattcact gtcggccgca gacgcgatcc tcttgttctt tccgcttctt
      601 ttcactccgt cgaaaaagca tatcgccagg caggcatcac tccgcgagac attgattttt
      661 ttgaagtaca tgacgcgttt agcatcatgg cagtattgtc tctggaagct tgtggatttg
      721 cagagagagg caaaggctgg cgggcgggca cggaaggaac gatttatccc ggcggaaaac
      781 tgcctctctc cactttcggc ggattgaaat cgaggggcca cccggtgggg gccacggctc
      841 tctatcaggc ttgcgagatc gtgcgacagt tgcgcggcca ggcgggccca aaccaggttg
      901 aaggaaaaac agcactgatg cagagtattg gaggtgcagg cacaaccata ctcacgcata
      961 tattccaaag gtagcactgg atgtccgata tagaaaacgt gccagaagaa tcgttcacgc
     1021 ccatactgtc tcccgcagaa ggcaggatga ttcctgttga gccagaagaa gatctgatct
     1081 cggacattga tttgagaaat ctaatggtgc agttgagccc aatgcatctc aaagacgtca
     1141 acgcgatgga gatggcagtc gaaaacttct acgtcttcca tgattccccc gaagctcttt
     1201 cgcatccaga gtctctgcaa tatctgggcg cccgcgatct ggaatggctg gcgagcaaac
     1261 atgcgattcg aatctttcaa tatatcacat gcgagggcga acaaaaaaga ctcagtgttc
     1321 atcgtgtggt ctttcgccct tccaatctga aagcggaagt gctcattcgt atcaaacgag
     1381 cgtgcattcg cacgtctgca tgggccatta tccgccatct gcaatttcgc gaacacgttc
     1441 tgagcagaat ggaaacatct ggcgagatca gcgtatggat tcgcaaacgt ttgcttggag
     1501 aattcgcggg aagtcccatg cgaaaattga gcgaaagcca ggtgaagagg ctgcctcaaa
     1561 tttttgagcg attggctttc gaacactttc tggatcaaga catcaaacct ctcaaggaac
     1621 tcaagcaggg catcttctat ctcttgacgc gaaagccgga agcaaacggg tttcttgagg
     1681 aaagttaccc acctcccgcg gacattgctg aataccttcg caatcgcatg cagcgtccta
     1741 ttctgactca actgtccgat gagtttcacg tgctcacacc tgcgattcca gcgccaggaa
     1801 tcgcaggacg ctctgaactg gattatatcc atgatctcta ttgcgaagcc tgcagggcaa
     1861 tactcatcgt cgggatggac gaagacgcgc agagagcgga atacgccgat ccaatggaac
     1921 tcatccatat catttcggcg cacgcctctt tctatgacga agagcattgg cccaggggaa
     1981 gggaatttct aagcgagctc aaagggatca tagagttgct tgtgttatcc acgcgaatga
     2041 gaagagaaat gatactcgga ggagttgcgc aaaagctgat atcttcgctg aacaatcgct
     2101 ttgatcccct cctgctggat gcacatgatc tagtgtcatc catgcccgat gacgcgcgca
     2161 ccctgtttcc ggatacaggc gctgcggcag ctgcattgat gagcattctg aaatccaacg
     2221 cggaacttgc attcttcacc gaacgacgcg gaagtggacc gcatgaacaa tcgtttgttt
     2281 tgttcagagc aaatctacca ggtgcatttg tgcaaaatcc ggaaagaaga acattctttc
     2341 acgcagtggc caaagaaaac ggctacccgc gcgggattta tgattttcta atcagcgtaa
     2401 aatcaagcga cccgaagaag cttgtggatg atcaactgga actatctcgc gccattcgcg
     2461 aatgggaaga tcacactcaa aaagtacaaa agcctaagtc cgttggatgg attgaaagac
     2521 ttacaaactg gattttgaaa cttctggggt ttggtgaatt tggaagaaga aaatcggaca
     2581 aatcaaagaa tacaacggat gcttctggtg cgaaggctaa gcacgcagca attccggccc
     2641 gagcgcaaaa ggcaatcgac tacgtggaa
//
LOCUS       DAORFF010000546         2665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_27049_length_2665_cov_5.019157, whole genome shotgun sequence.
ACCESSION   DAORFF010000546 DAORFF010000000
VERSION     DAORFF010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27049_length_2665_cov_5.019157"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PKK76_17135"
     CDS             complement(<1..642)
                     /locus_tag="PKK76_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70257.1"
                     /translation="MHSKINPALILLAGVGLLYCNKPVPVQELAQARDEIAMAEEEHA
                     PEYSASNFQGARDALLASHQKLGSEEYDNAKQKAIDSFNQARAARVEASPKYTEATRA
                     TAEKTIAQADQAYAEELAKDDFSAAKKLYGEGTTAQGQAKAATGDAQLDTYRQAYRKF
                     EAANSAGDKARTTALAQKGQMMQSLAGVQAILDKAERYGAKDLAPQEFQSATSD"
     gene            complement(685..1020)
                     /locus_tag="PKK76_17140"
     CDS             complement(685..1020)
                     /locus_tag="PKK76_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL70258.1"
                     /translation="MEITRRASGDIVILDVNGEIDLYNAPEIKELINKLIEEQKYNVI
                     INLDRVSYIDSSGIGALISSLSNLKKYQGGLKIINVSGSVRKVFELTKLTSFFEIYDS
                     EADAVAAFK"
     gene            complement(1140..2297)
                     /gene="tgt"
                     /locus_tag="PKK76_17145"
     CDS             complement(1140..2297)
                     /gene="tgt"
                     /locus_tag="PKK76_17145"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNL70259.1"
                     /translation="MMSFYKEESRDGEARTGKLSFPSDAGGTIEVETPCFMPVGTRAS
                     VKGLWQEDLEEIGYRLILGNTYHLYLRPGSSQIRELGGLGRFMSWSGALLTDSGGYQM
                     FSLSDRMQLDDDGVNFKSHLDGSEHRFTPESAIDIQTDLRSHIMMVLDDCPPADADES
                     RIQASLVRTHSWARRSIDHHNKLCEDGRIDPNRSRMFGIVQGGTSQSARAQSLQAIGE
                     LPFSGVAVGGLSVGESRESFYDVLNFLGGRMDPARPRYLMGVGTVPDFIEAVRNGFDM
                     FDCVLPTRNARNGQGLTSQGKLRIRNQSYKNDETPLDPACKCRVCRRYSRAYIRHLFM
                     SDEMLGPQLMSFHNLAFFYTFMEEMRGSIRAGRFATFYLQWKEWDKNGPDE"
     gene            complement(2317..>2665)
                     /locus_tag="PKK76_17150"
     CDS             complement(2317..>2665)
                     /locus_tag="PKK76_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70260.1"
                     /translation="DRKKYDESLKTAGTLLEKLDPMMRDPKTYLKKLEKYRVGERTVA
                     LLTAGEGEYHRVAVLYYGALLRDIRNSPVIEDADRREKCKQRIEYHATKFLLHNAQSD
                     PEVQRFLSEAKGL"
BASE COUNT          638 a          707 c          639 g          681 t
ORIGIN      
        1 atcagaagtg gcagactgga attcctgcgg agccagatct tttgcgccgt atctttccgc
       61 tttatccaga attgcctgca cgcccgcgag agactgcatc atctgacctt tttgagccag
      121 agcagtcgtg cgcgccttat cccctgcaga gttggctgct tcgaatttgc ggtacgcctg
      181 acgatatgtg tcgagctgag catcgcctgt ggctgccttt gcctgaccct gcgcagtagt
      241 tccttctccg tacagcttct ttgcggcgga gaaatcatct tttgcaagct cttccgcata
      301 agcctggtct gcctgagcga tagtcttttc tgccgtagcg cgagttgctt ctgtatattt
      361 gggagaagcc tctacgcgag cagcgcgagc ctgattgaat gaatcaattg ccttttgctt
      421 tgcgttgtca tattcttccg acccaagctt ctggtgagaa gcaagcaacg catcacgcgc
      481 gccctggaag ttagaagcag aatattccgg agcgtgctcc tcttctgcca ttgcaatttc
      541 atcacgtgcc tgtgctaact cctgcaccgg aacaggcttg ttgcagtaca gcaagcccac
      601 accagccaga agaatcaaag cgggattaat tttggagtgc ataggttctc tccttgagtg
      661 aatgcgcccg aagagcgcgc ggcatcactt gaacgccgcg actgcgtcag cttcggagtc
      721 gtagatctcg aagaatgacg taagcttcgt cagttcgaac accttacgaa cggagcccga
      781 aacattgatg atcttgagcc cgccctgata tttcttcagg ttggaaagac tggagatcag
      841 agcaccaatg ccagacgagt caatgtaaga gacacggtcc aggttgatga tgacgttgta
      901 cttctgctct tcgatgagtt tgttgatgag ttctttgatt tccggggcat tatagagatc
      961 gatctccccg ttgacgtcca gaatgacaat gtcaccgcta gctcttcttg ttatttccat
     1021 atggttgaga caggctcctc tatttgtccg gaatcctcga aagataccgg actttccacg
     1081 atccctgtca acataaaaaa ttcaagcgca aaggggaaaa atgagcaaag aagtttcagt
     1141 cactcatctg gaccattttt gtcccattcc ttccattgaa gataaaaagt ggcaaaccga
     1201 ccggctcgaa tcgaccctct catctcttcc atgaatgtgt agaaaaaggc cagattgtga
     1261 aacgacataa gctgggggcc cagcatttcg tctgacatga acaaatgccg tatgtacgca
     1321 cgcgaatatc gcctgcaaac acggcattta catgcaggat cgaggggggt ttcgtcattt
     1381 ttgtatgact gattgcgaat gcgaagcttc ccctgtgacg tgaggccctg accatttctg
     1441 gcatttcgcg ttggcaaaac acaatcgaac atgtcgaagc catttcgcac tgcttctatg
     1501 aaatcgggaa ctgttccaac acccattaag tatctcgggc gagccgggtc catacgaccg
     1561 ccaagaaaat tcaaaacatc gtaaaaagac tctcgcgact ctcctacgga aagcccgccc
     1621 acagcgactc cgctgaaagg aagttccccg atcgcctgaa gagactgcgc tcgggcggac
     1681 tgcgatgtcc ctccctggac aatcccgaac attctgctac gattcgggtc aatgcggccg
     1741 tcttcgcaga gtttgttgtg atggtctata gatcgtctag cccagctatg agtacgaacc
     1801 agagaagcct ggatgcgcga ttcgtccgcg tccgccggcg gacaatcgtc cagaaccatc
     1861 atgatgtgcg aacgcaagtc tgtctgaatg tctatggcag attctggagt gaagcgatgt
     1921 tcgcttccgt ccagatgact tttgaaattc acaccgtcat catccagctg catgcgatca
     1981 gaaaggctga acatctggta tccaccgcta tccgtcaaaa gtgcgccgga ccaggacatg
     2041 aaacgaccca gcccaccgag ttcccgaatc tgcgatgaac cagggcgaag atacagatga
     2101 tacgtatttc cgagaatcaa gcgatagcca atctcttcca ggtcttcctg ccagagtccc
     2161 ttgacgctcg cgcgtgttcc cacaggcatg aagcaaggcg tttctacctc gattgtgcca
     2221 ccagcatcag aaggaaatga aagcttcccc gtacgagctt ctccgtccct cgattcctct
     2281 ttatagaaac tcatcacgaa gttgtgcgcg aaagcatcag agaccttttg cttcgctcaa
     2341 gaatcgttgg acttccggat cgctttgagc gttgtgcaag agaaactttg ttgcatggta
     2401 ttcgattcgt tgcttgcatt tttcgcgacg atccgcatcc tcaatgacag gggagttgcg
     2461 aatgtctcta agcaaagcac cgtaataaag aacggccact ctatggtatt cgccttctcc
     2521 tgcagtcaga agtgctacag ttctttctcc gacgcgatat ttctcaagct tctttaaata
     2581 ggtctttgga tctcgcatca ttggatcgag tttctcaagc agagtccccg cagttttcaa
     2641 tgattcgtca tacttcttgc gatct
//
LOCUS       DAORFF010000547         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_27212_length_2657_cov_3.410453, whole genome shotgun sequence.
ACCESSION   DAORFF010000547 DAORFF010000000
VERSION     DAORFF010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27212_length_2657_cov_3.410453"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..2071
                     /locus_tag="PKK76_17155"
     CDS             68..2071
                     /locus_tag="PKK76_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70261.1"
                     /translation="MILAAVFAVIVLAFALAGPVERAFTRSLPLAEIKSQIIAQAKET
                     AGIELSIRGARFEILQGVVLTGVSARFGNDPVFFHGENLIVEISPFKAMKKNLTPQIR
                     IAGGRIVPENLPRARLFELLDVLRKNEGGDWKLDFDGILIDSKPARYTLSGRLTPGDN
                     RVDGRLDVSLKGKRVIKSDFRILKAHLGSSFKVNSLPLSEAASYFPSILAKSAGSVSG
                     KGTLSMDSSSLAYDFHGEMADFESGIVRGKHEARINLNGGHVWKENIGLIASFDMSDP
                     TFSIRMTEKLASQGIAARELEGRIQSLPYFQTGVLNFKIKSRFRALQEVREQIEEITA
                     SLENAAYIPAHDVNFQIDAASISLSSDLKFDAKGKALDHAFTLHASGPFRFGDSPDLF
                     SGGPLVIEGEVATLDLPRIARAMFGAHSHLKGIGTAEDAEKAEDTGPLWTQRRKTQAQ
                     ANLLSYFSMNGSIVAKGIAGVTNESVPINISKNGGGISARAGYQKDGFRLEGDYALGF
                     DSDLPHQTIHLKYDNEQNNVSMEYLTGDEQSPRSISLDYNSSMDGLLPADLFFKSTST
                     MYFRARGVDTRKLDPAPLVFSIAGIKDSKIDELEFQRTTEGGRTMWTQVHASRGEANW
                     TGAGDFSSQEGGKATFFVNAPGGYSRRIDLGVTEAGKWYPREN"
     gene            2050..2448
                     /locus_tag="PKK76_17160"
     CDS             2050..2448
                     /locus_tag="PKK76_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70262.1"
                     /translation="MVSTRKLVLAFSLLAMACGGKPQVDRGLSVSPEKLSGRFAMVPS
                     ATQEEIRFKEDGLVEMKSEESSAKGLYALDASSLRFRFENGDYGIFLRSAFHPREWRG
                     FYRGEVRILKRLEGAKNLDRSESEGTTSKP"
     gene            2445..>2657
                     /locus_tag="PKK76_17165"
     CDS             2445..>2657
                     /locus_tag="PKK76_17165"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNL70263.1"
                     /translation="MKFSEVFEEKIWNASNILTMTRMILLPVYVWLGGAYREDPGGPM
                     LWVLLSLISGAVISDFLDGFLARKLHQ"
BASE COUNT          681 a          643 c          702 g          631 t
ORIGIN      
        1 atgaacagtt gcaggaatct tccagtgagg aaagattggc gctgggctca tgaataagaa
       61 gaccatcatg atcttggcgg ccgtttttgc ggtgatcgtc cttgcctttg cgcttgccgg
      121 tccggtggaa cgtgctttta cgcgcagtct gcctcttgca gaaatcaaat cacagatcat
      181 tgctcaggcc aaagagactg cggggattga actgtccatt cgaggcgcac gattcgagat
      241 tcttcagggc gtggtgctca cgggtgtgag cgcgcgattc ggaaatgatc ccgttttctt
      301 ccatggagag aacttgatcg ttgagatatc gccattcaag gcaatgaaaa aaaatctaac
      361 tccgcagata cgaattgcgg gtggcagaat cgtgcctgag aatttaccgc gagcaagact
      421 ctttgagctt ctcgatgtac tgagaaagaa tgaaggcgga gattggaagc ttgacttcga
      481 cggcatcctg atcgattcaa agcctgccag atacactcta tccggtcgtc tcactccggg
      541 agacaaccgc gtggacggga ggctggatgt ttcgctcaaa ggaaaaaggg tcatcaagag
      601 cgactttcgc attctcaaag ctcacctcgg cagtagtttc aaagtgaatt cacttccgct
      661 ttcggaagca gcttcttatt tcccgagcat actggcaaaa tcggcgggga gcgttagcgg
      721 caaaggcact ctttcgatgg attcatcttc ccttgcctat gatttccatg gagaaatggc
      781 agacttcgaa tctggaatcg ttcgcgggaa gcatgaagcg cgcatcaatc taaacggagg
      841 ccatgtctgg aaagaaaaca ttggcctcat cgcatctttt gacatgagtg atccaacatt
      901 ttcgattcga atgacggaga agcttgcatc ccagggaatt gctgcgcgcg agctcgaagg
      961 tcgaatccag tctcttccgt attttcagac gggagtattg aatttcaaaa tcaaatccag
     1021 atttcgtgct ctccaggaag tgcgagaaca aatcgaagag atcacggcga gcctcgagaa
     1081 tgccgcatac attcctgcac atgatgtcaa ttttcaaatc gatgctgcga gcatttctct
     1141 ttcatcggat ttgaaatttg atgcgaaagg aaaagcactc gatcatgcgt tcacacttca
     1201 cgcttcgggt ccctttcgtt ttggagacag tcctgactta ttttccggtg gtccgcttgt
     1261 gattgaaggg gaagtagcaa ctctggattt accgcgaatt gcccgcgcga tgtttggagc
     1321 acactcccac ctcaaaggaa tcggcacggc agaagatgcg gaaaaagcgg aagacacagg
     1381 tcccctctgg actcagagaa gaaagactca ggctcaggca aacttgctca gctatttcag
     1441 catgaatgga tccatcgttg cgaaaggaat cgcgggcgtt acaaacgaat ccgtccccat
     1501 caacatcagc aaaaatggcg gaggaatcag tgctcgcgcc ggataccaga aggatggatt
     1561 tcgcctggaa ggcgattacg ctctcgggtt tgattcagac ctcccgcacc agaccatcca
     1621 tctgaagtac gacaacgagc aaaacaatgt atccatggaa tatctaaccg gagacgagca
     1681 gagccccagg tctatatcgc tggattacaa ctcatccatg gatggacttc tgcccgcgga
     1741 tctttttttc aaatctacaa gcacaatgta tttccgcgcg cgcggtgtgg acactcgcaa
     1801 gctcgatcca gcgcctcttg ttttttccat cgcaggtatc aaggatagca agatagacga
     1861 gctagaattc cagagaacta cagaaggtgg cagaaccatg tggacccagg ttcacgcatc
     1921 caggggagag gcaaactgga cgggcgcagg cgacttctcc agccaggaag gcggcaaagc
     1981 tacttttttt gtgaatgcgc ctggtggcta ttccaggcgg attgatcttg gagtgactga
     2041 ggcaggaaaa tggtatccac gcgaaaacta gttctcgcct tctcacttct cgcgatggca
     2101 tgcgggggaa aaccccaggt agatcgcggc ctgtctgtct ctccagaaaa actgagcggt
     2161 cgatttgcca tggttcccag cgccacgcaa gaagagattc gtttcaaaga agacgggctc
     2221 gtagaaatga aatcggaaga atcatccgcg aaaggtctgt atgctctcga cgcatcttct
     2281 ctcaggtttc gatttgaaaa tggagactat ggaatatttc tccgcagcgc atttcatccc
     2341 agagaatggc gaggctttta ccgcggtgaa gtgcgcattc tgaaaaggct ggaaggggct
     2401 aaaaaccttg accgatctga atcggagggc accactagca agccatgaaa ttctcagagg
     2461 tgtttgaaga gaagatctgg aatgcttcga acattctgac tatgaccaga atgatcctgc
     2521 tgcccgtcta cgtgtggctg ggtggagcct accgggaaga cccgggcggc cccatgctgt
     2581 gggtcctcct ctctttgatt tccggcgctg tgatctctga tttcttagat ggatttttgg
     2641 cgcgcaaact gcatcag
//
LOCUS       DAORFF010000548         2651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_27318_length_2651_cov_4.050847, whole genome shotgun sequence.
ACCESSION   DAORFF010000548 DAORFF010000000
VERSION     DAORFF010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27318_length_2651_cov_4.050847"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="PKK76_17170"
     CDS             complement(<1..1048)
                     /locus_tag="PKK76_17170"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL70264.1"
                     /translation="MMARLSSIALGALLLIACRSPVDCRPADLSCNGLASLLLYSRVS
                     LVRAAYLTDNVSNLLYSYSIDPTTGQPSLIGSIGTQVLPRGVAVHPAGYVYAANETGG
                     SISIYRILPGYSVSLVTHFNVGGGTPSALAINSAGTLLYVTRQGVSLVNSYWIDASTG
                     LLSSTGFTGTTDATTNQISLHPTLPYAFTGGTGATSYGFLLGANGSFTTIPGAVTATD
                     TFYPLITPNGRYLLISDNGQSRVELFSVSQQNGALTALSPAFFSVGAAAGVSFVDASG
                     TSLYVCIPISNSMRTFPMNSTTGQPSAQSQAFSVSGGPYGLAMDPTNTYLFAAGGTTL
                     YVFKRDSAGTLTQIA"
     gene            1180..1425
                     /locus_tag="PKK76_17175"
     CDS             1180..1425
                     /locus_tag="PKK76_17175"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNL70265.1"
                     /translation="MLNLIWAIVVGFFVGLLARFLLPGNDKMGFILTTICGVVGGVVG
                     TYIAPYIPFLPGGTIGSLIASTIGAIIILVVLRIVRR"
     gene            complement(1517..2113)
                     /locus_tag="PKK76_17180"
     CDS             complement(1517..2113)
                     /locus_tag="PKK76_17180"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNL70266.1"
                     /translation="MKTQSKKRKVPYGMAREDIAAAARDLFYEQGLATGLDEILKRAD
                     VSKPTFYHHFGSKEELESQYLTAQAEFLRTSVDRIAGKSRDACQFIRRWMEFIRRRAS
                     SPTFTGCPLGNFAIEAKSAHNARVLREFGLSIEKLEDTFKAKGIQATRARELAPLIFM
                     IYEGALLMCSATEERSHLSRATELMVRLVREKKSPTLK"
     gene            2183..>2651
                     /locus_tag="PKK76_17185"
     CDS             2183..>2651
                     /locus_tag="PKK76_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70267.1"
                     /translation="MMTEKMESIQKPGRIESSYRMETVEGLELWRLGRDRFSDFNQFV
                     YGVYSEAFGENGKIPFEKRDIEAASDEHFDHARVCAVIHPDGTLLGTWGLILKERSDS
                     ALLPIEKAFSLTTEEIISKMNVTEVRYIFNGWRTAVDKNALLDHGFAANRSMFV"
BASE COUNT          663 a          632 c          702 g          654 t
ORIGIN      
        1 gggcgatttg tgttaaagtg cctgccgaat ctcgcttaaa gacatacagc gtggttccac
       61 ctgcggcgaa gaggtaggtg ttggtcgggt ccatggccaa tccatatggt cccccgctta
      121 cgctgaaggc ttgcgattgc gcagacggct gacctgtcgt cgagttcatc gggaaggttc
      181 gcatcgaatt cgaaatggga atgcaaacat acaggcttgt tccgctggca tcgacgaatg
      241 aaactccagc agcagccccc acactaaaga atgcaggcga aagtgcggtg agggctccgt
      301 tctgctgcga tacagaaaac agttcaacgc ggctttgtcc attgtctgag atgagcagat
      361 aacgtccgtt tggcgtaatc agaggataga acgtatctgt ggccgttact gcgccaggga
      421 tcgtcgtaaa agagccattt gcgccgagta gaaacccata gcttgtagct cccgttcctc
      481 ctgtgaatgc ataaggcaat gtaggatgaa gcgaaatttg attggtggtc gcgtctgtcg
      541 tgcccgtaaa accggtggaa gacagaagtc cggtagatgc atcgatccag tagctgttca
      601 ccaacgagac cccctgtctg gtcacgtaga ggagagtgcc tgccgagttg atggccaggg
      661 cggatggagt tccaccacct acattgaagt gcgtaaccag gctgaccgaa tagccaggta
      721 agattctgta aatcgatatg ctgcctcctg tttcgttggc agcatataca tatcctgctg
      781 gatgaaccgc aacacctctg ggcaggacct gggttccaat cgagccgatc agggaaggct
      841 ggcctgtagt cggatcaatg gaataggaat acagaaggtt ggacacgttg tctgtgaggt
      901 acgcggccct gaccagtgag actcgagagt aaagcaacaa ggatgcaagg ccattgcagc
      961 tcaagtctgc cggccgacaa tccacggggc tcctacaggc gatcagaagc agggcgccca
     1021 gggcgatgga ggacagacgg gccatcattt tccaattgaa ttagacgatc caaagcccgc
     1081 ctctcttaca cacaaatcgg cagttaactt ccatgcaagc acttgacaga attcacaaag
     1141 gagctcaaat acatgcaaaa aggttcagga gctaaataaa tgttaaatct gatctgggca
     1201 atcgtcgttg gtttctttgt cggtctactg gctcgtttcc tccttccagg aaatgacaag
     1261 atgggcttca tactcacaac gatttgcggt gtcgttggtg gtgttgtggg cacgtacatc
     1321 gcgccttaca ttccgtttct tccaggtggg actatcggaa gcctgatcgc gtctacgatt
     1381 ggggcgatca tcattctcgt tgtcctgcgc atcgttcgca ggtaaacgat cttgcggccg
     1441 gcctatggct ggtcgcaaca gtccttcaca ataccacctt tcaatggtta ggcgatcatt
     1501 cgccggctgt cttttatcat ttcaaagttg gtgatttttt ttctctcaca agtctcacca
     1561 tcaattcagt agcccggctc aagtgagatc gttcttccgt agccgaacac atgagcaggg
     1621 caccttcata gatcataaaa atcaacggcg ccagctctcg agcacgcgtc gcctggatcc
     1681 cttttgcttt gaacgtgtct tctagctttt caatagaaag accaaactcg cgcagaaccc
     1741 tcgcattgtg tgcggatttt gcttcgatgg caaaattgcc aagagggcat cctgtgaaag
     1801 tgggggaact ggctcttctg cggatgaatt ccatccatct gcgaatgaac tgacaagcgt
     1861 ccctggattt acccgcaatg cgatctaccg aagtgcgcag aaactctgcc tgggcagtca
     1921 ggtactgaga ttccagctcc tctttgcttc caaagtgatg atagaaggtg ggtttgctta
     1981 cgtcagcgcg tttgaggatt tcatcgaggc ctgtggcgag tccctgctca tagaatagat
     2041 ctctggccgc ggccgctatg tcttctctgg ccattccgta cggaaccttg cgttttttgg
     2101 attgcgtctt catgcaaata atacttactg gtcagtaata gtctttggat aaaaaaatca
     2161 accagatttc aggagaaaag caatgatgac tgagaaaatg gaaagcatac aaaagcccgg
     2221 tagaattgag tcttcgtaca ggatggagac ggttgagggg ctcgaactat ggcgactggg
     2281 aagagatcgc tttagtgact tcaaccagtt tgtatacggg gtttacagcg aagcatttgg
     2341 tgaaaacggc aagattccgt ttgagaaaag agacatagag gcagcaagcg atgaacactt
     2401 tgatcatgcc agagtgtgcg ctgtcattca tcctgatgga actctccttg gaacctgggg
     2461 cctgattcta aaagaaagaa gcgattctgc cctcttgccg attgaaaagg cattctcact
     2521 gactacggaa gagatcattt ctaaaatgaa tgtcaccgaa gttcgctaca tcttcaatgg
     2581 ctggcgcaca gcggtggaca aaaatgcgct cctggatcat gggtttgccg caaatcggtc
     2641 tatgtttgta t
//
LOCUS       DAORFF010000549         2651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_27327_length_2651_cov_2.712635, whole genome shotgun sequence.
ACCESSION   DAORFF010000549 DAORFF010000000
VERSION     DAORFF010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27327_length_2651_cov_2.712635"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2651
                     /locus_tag="PKK76_17190"
     CDS             <1..>2651
                     /locus_tag="PKK76_17190"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL70268.1"
                     /translation="IFAVMKTQGNKLVLQESSRLIEQIGNNVVAKLAERSRQIEALCR
                     SEASVGEQLPRDARLFKQVVPTMINFRGDMNVAGGGIWPEPFVFTPGVEKRSFFWGRD
                     EHGSLQYFDDYNTNERGYRHDEWYPVVRYSRPGECFWSKSYMDPYSYQPMVTCTVAMR
                     DTQDQFVGVATIDLKLEGLHAFMENMRQRTGGYIFLLDRNNKFLTFPKEEDVEVIGKD
                     EKGNKTRDFLTIADMAKKQPLFAPIADAVHAMNKDITERAKSTKGFRADIATKIDHDS
                     DQIDAEEAEFIAAAIADPLAEQTKDTSLYRKFEIEKDYITGESAIVFLFHVPQSYWKV
                     IIVKPLREAGAVASQITSVLITMITITVLVGVALAALLLHRFFTIPLRNTTEAVTNIG
                     HVVSEKQFQELDAHKIKSVREDELGQLSGVVNSLASELQSSYGSLLELNQNLEQKVED
                     RTRRLQQTLEEVSELKKRQDGDYFLTSLLIKPLAVNRVEGNHTDVRFLMKQKKSFIFK
                     NREDELGGDICLADTIHLHGRTFTVFLNGDAMGKSIQGAGGALVLGAVARMMIERTKL
                     TMSMQNLYPERWLKNAFIEFQKMFESFDGSMLVSVVLGLIDDDSGVLYVINAEHPRTV
                     LYRNGRAAFLEEDTIHRKLGIGGLDGKLQIGTYQLETGDVIIAGSDGRDDILIGMGQD
                     GTRTMNSDETLFVKHVEAGNGNLDQIYSGLVESGEITDDLSLLRVEYRKEASPERKAS
                     QEAISRARALAKGGDQTGGVRELEGALGRDPLNPELVRPLIKYYLEKKNFARTAEIAE
                     PLAEAGHLDNEFAFVTAHCRKKLNRLKEAADMAERVYLREPELAKNLFLLAEVYARMG
                     NIARGADFLDKAEKLDPGNPAVAKVRA"
BASE COUNT          776 a          626 c          697 g          552 t
ORIGIN      
        1 atcttcgccg tgatgaaaac ccagggaaac aagctggttt tgcaggaatc gtctcgcctc
       61 attgagcaaa ttggaaataa cgtggtcgcc aaactcgcag agcgctctcg ccagatagaa
      121 gcgctttgta gaagcgaggc aagcgtcgga gaacagctgc cacgcgatgc gcgtctcttc
      181 aagcaggttg tacctactat gatcaacttc cgcggagata tgaacgtggc cggcggagga
      241 atctggccgg agccatttgt attcactccg ggtgtagaaa agagaagctt cttctggggc
      301 cgcgacgaac acggatccct ccagtacttc gatgattaca acacgaatga acgcggctac
      361 agacacgacg aatggtaccc tgttgtccgc tactccaggc ccggggaatg cttctggagt
      421 aaatcgtaca tggatcctta ctcctaccag cccatggtta catgcactgt ggccatgcgg
      481 gatactcagg atcaatttgt aggagtggcc acgattgatc tgaaactcga aggactccat
      541 gcattcatgg agaacatgcg gcaaaggaca ggaggataca tctttctgct ggatcgaaac
      601 aacaagttcc tgacgtttcc aaaagaggaa gatgtagaag tcattggcaa agatgaaaaa
      661 ggaaacaaga cgagggactt tctgactatc gcagatatgg caaagaaaca accgttattc
      721 gctcctatcg cagatgcagt tcatgcgatg aacaaagaca tcacagaacg cgcaaagagt
      781 acaaaaggat tcagggcaga catcgcgaca aaaatcgatc atgatagcga tcagatagat
      841 gcagaagaag cagaattcat agccgcagcc atcgcagatc ctcttgcaga acaaacaaaa
      901 gatacatctc tctacagaaa gttcgaaatc gagaaagact atatcacagg cgaatccgcc
      961 attgtgtttc tgtttcacgt tccgcaatcc tactggaaag tgatcatcgt aaaacctttg
     1021 cgcgaagcgg gcgctgttgc ttcgcagatt acttctgttc tcatcacaat gattacaatc
     1081 acggttcttg tgggtgtagc tctcgcagcg ctcttgctcc atagattctt caccatccct
     1141 ctgcgaaata caaccgaagc ggtaacgaac atcgggcatg tagtatcgga aaagcaattt
     1201 caagaactgg atgcgcataa gatcaaaagt gtacgcgaag atgaactggg ccagctttca
     1261 ggagttgtga attctcttgc ttctgaattg cagtccagtt acggatcgct cctcgagctc
     1321 aatcagaacc tggagcagaa ggtagaagat cgcacgcgaa gattgcagca gactctggaa
     1381 gaagtgagcg aactcaaaaa acgccaggat ggagactact tccttacatc tcttctcatc
     1441 aaaccgctcg ccgtgaatcg ggtggaaggc aaccacacag acgtgcgttt cctcatgaag
     1501 caaaagaagt cgttcatctt taaaaaccga gaagatgaac ttggcgggga catttgtctg
     1561 gcagatacga ttcacctgca cggcagaaca ttcactgttt tcttgaatgg agatgcgatg
     1621 ggcaagtcca tccagggcgc aggcggagca ctggtgcttg gcgccgtggc gcgcatgatg
     1681 attgaaagaa ccaagctcac catgagtatg cagaatctgt atccggaacg ctggttgaaa
     1741 aatgcgttca tcgaattcca gaaaatgttc gaaagcttcg acggcagtat gctcgtatcc
     1801 gttgtcctgg gtctcataga cgacgacagc ggagtgcttt acgtcatcaa cgcggaacat
     1861 ccacgcactg ttctatatag aaacgggaga gcagctttcc tcgaagaaga taccatccac
     1921 agaaagctgg gcattggtgg actggacgga aaacttcaga ttggcacgta tcaactggaa
     1981 acaggggatg tgatcatcgc aggatcggac ggcagagacg acatactcat tggaatggga
     2041 caggacggaa cccgcacaat gaacagcgat gaaacgctgt ttgtaaaaca cgtggaagca
     2101 ggaaatggaa atctggatca aatctactcc ggtctagtag aaagcgggga aatcacagac
     2161 gacctttcgc ttctgcgcgt ggaatacagg aaggaagcaa gccccgaacg aaaagcatct
     2221 caggaagcaa tttccagagc gcgcgcgctt gcaaagggag gagatcagac aggtggagtg
     2281 cgtgaactcg aaggtgctct gggaagagat ccactgaacc ctgaacttgt gcggcccctc
     2341 atcaaatact atctagaaaa gaagaacttc gcacgcactg cagaaatcgc agaaccgctc
     2401 gcagaggccg ggcatctgga caatgaattc gcattcgtca cggctcattg ccgcaagaaa
     2461 ctcaatcgcc tcaaagaggc agcggacatg gcggaaagag tgtatctgcg agaacctgag
     2521 cttgcaaaga atttgtttct tctcgcggaa gtttatgcga ggatgggaaa cattgcgcgc
     2581 ggtgcagatt tcctggacaa agcagagaag ctcgatccgg ggaacccggc cgttgccaaa
     2641 gtgcgcgcga t
//
LOCUS       DAORFF010000550         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_27337_length_2650_cov_4.107514, whole genome shotgun sequence.
ACCESSION   DAORFF010000550 DAORFF010000000
VERSION     DAORFF010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27337_length_2650_cov_4.107514"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PKK76_17195"
     CDS             <1..239
                     /locus_tag="PKK76_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70269.1"
                     /translation="AELRHLYWQKEQTGISSKMKIKVIIQKAGAQALGALKSVMTTLE
                     RSQMAEIVKTEADCDGATQEQICREFIARIRRAK"
     gene            236..796
                     /locus_tag="PKK76_17200"
     CDS             236..796
                     /locus_tag="PKK76_17200"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNL70270.1"
                     /translation="MIEFAGYVVLALALTMDTLAVSVVCGNFLKEFRVREALRLGLSM
                     ALVQGAFSLIGVLAGSFAARYLEKVDHWIAFVLLVGIGIKMIRDAYKSDDDEEAPSLA
                     LRVILLMSVATSIDSIMACAGLALTDPHLINAVPVIAVMTLLISLVGTYAGAKLGAAI
                     GSHAGALGGIVLIGLGSRILYLHFAA"
     gene            complement(806..1801)
                     /gene="galE"
                     /locus_tag="PKK76_17205"
     CDS             complement(806..1801)
                     /gene="galE"
                     /locus_tag="PKK76_17205"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNL70271.1"
                     /translation="MDVLVVGGCGYIGSHACKILSEKGYNVINLDNLSRGHREFAQWG
                     TLYEGDLGNSDLLDQIFSRHKIEVVMHFAALAYVGESVTKPDIYYQNNVQKPLVLLDA
                     MRKHKVEYFIFSSTCATYGELTPESIPVREDCAQNPVNPYGRSKYFFEQILLDYEKAY
                     GLKSLFFRYFNAAGADPSGAIGEWHEPETHLIPLIFQATLGVVPELTVFGSDYVIPGG
                     NGDGTAVRDYIHINDLVQAHILGIDYLRRINKTEAFNLGNGKGFSVLEMIQTGERITG
                     MKVPYKLGPRRAGDPPVLVGAAEKARNLLGWKPEFPHIERIMETAWNWEKKFRKK"
     gene            complement(1801..>2650)
                     /locus_tag="PKK76_17210"
     CDS             complement(1801..>2650)
                     /locus_tag="PKK76_17210"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNL70272.1"
                     /translation="NKHKTLVDQFAYPSGGTGMIYERMADFIRSKGSNVMLKTPVQRV
                     ITENGKATAIETEGGRTEKYDHVVSSMPITLLVSRLPEVPEPIKEFSKKLKFRNTVLV
                     YVEVKAKDLFPDNWLYVHSDNLLMGRLTNFRNWVPELYGKEESTIMVLEYWCYDEDAF
                     WTRTDEDLIELAKKELKSTGLIGNAEMPRGHVHRIHRCYPVYDRGYKERLKPVEDYLS
                     SVGNLSVIGRYGAFKYNNQDHSILMGILASENILHGKNNNLWDINTDYEYQESSKITK
                     TGLVKG"
BASE COUNT          590 a          619 c          662 g          779 t
ORIGIN      
        1 gggcggagct cagacatctg tactggcaga aagagcagac gggcatcagt tctaagatga
       61 agatcaaggt gatcattcag aaggcgggtg ctcaggcgct cggcgcattg aaatctgtca
      121 tgactactct ggagcgctct cagatggcag agatagtaaa aacagaagca gattgcgatg
      181 gagccacgca ggaacagatt tgcagggaat tcatcgcgcg gatcaggcgg gcaaaatgat
      241 agaattcgca ggttacgtgg ttctcgctct cgctcttaca atggatactc tggcggtctc
      301 cgtggtttgt gggaattttc taaaagagtt tcgcgtacgc gaagcgcttc gtcttggttt
      361 gagcatggct ctggtccagg gggccttttc tctcattggc gttctggccg gatctttcgc
      421 tgcccgctat ctagaaaaag tggatcactg gatcgccttt gtgctgttag tcggaattgg
      481 cattaagatg attcgagatg cctataaatc tgatgatgac gaagaggcac catctctggc
      541 tttgcgagtc attttgctca tgtctgtggc aacgagcatt gattcgatca tggcttgcgc
      601 aggacttgcc ctcacggatc ctcatctaat caacgcagtt cccgtcatcg cagttatgac
      661 actgttgatt tcactggttg gcacgtatgc gggagcaaaa ctgggagcgg cgattggttc
      721 tcacgccggg gcactcggtg gcattgtgct cattggactc ggttccagaa ttctctattt
      781 gcatttcgca gcctagctgc ggcgcttatt tctttctgaa tttcttttcc cagttccagg
      841 cagtttccat aattcgctca atgtgaggga attctggttt ccatcccagt agatttcgcg
      901 ccttttctgc cgcacccacc agaacaggag gatctccggc tcttcttggg ccgagtttgt
      961 agggaacttt cattcctgtg attctctcgc ctgtctgaat catttccagt acagagaacc
     1021 ctttaccatt tcccaggttg aaggcttctg ttttgttgat tcttcttaaa taatcgattc
     1081 cgagtatgtg agcctgtacg agatcgttta tgtgaatgta atcgcgaact gcggttccgt
     1141 ctccattgcc gcccggaatg acgtaatcag agccaaatac ggtgagttcg ggcactacac
     1201 ccagagttgc ctggaaaatc agagggatca ggtgcgtctc cggttcgtgc cattcaccaa
     1261 tggcgcctga cgggtccgcg cctgcagcat tgaaataccg aaaaaacaag gatttcagtc
     1321 cgtatgcttt ttcgtagtcc aggagaatct gttcaaagaa gtatttagat ctgccgtaag
     1381 gattcaccgg attctgcgcg caatcctctc gcactggaat gctttccgga gtgagttctc
     1441 catacgttgc gcacgtggaa gagaaaatga aatattctac tttgtgcttt cgcatcgcat
     1501 caagcaagac gagcggcttt tgcacattat tctggtagta aatatccggt tttgtgactg
     1561 attctcccac gtaagccagt gctgcaaaat gcatcacgac ttcaattttg tgtcgcgaaa
     1621 aaatctgatc gagtagatcc gaattcccga gatctccctc gtacagagtg ccccattgcg
     1681 cgaattctcg atgaccccgg cttagattgt cgaggttgat tacgttgtag cctttttccg
     1741 aaagaatctt gcatgcatgc gagcctatgt agccgcatcc gcctacaacc agaacgtcca
     1801 ctatcctttt accagccctg ttttggtgat tttggaggat tcctggtatt cgtagtcagt
     1861 gttgatatcc cacagattgt tgtttttgcc gtgaagaatg ttttcagagg ccaggattcc
     1921 cataagaata gaatgatcct ggttgttgta cttgaacgcg ccatatcttc cgatgacgct
     1981 gaggttccca acactcgaga gatagtcttc cacgggctta agtctttctt tgtaaccacg
     2041 gtcatagaca ggatagcagc gatgaatgcg atgaacatgc ccgcgaggca tttccgcgtt
     2101 gccgatgagc ccggtggatt tgagttcttt tttagcgagc tcgattaagt cttcatctgt
     2161 gcgagtccag aatgcgtctt cgtcatagca ccagtattcg agaaccatga tcgtggattc
     2221 ttctttgccg tagagttctg gtacccagtt gcgaaagttc gtcagtcgtc ccatgagtag
     2281 attgtctgaa tgcacataga gccagttgtc cggaaataga tcttttgctt tcacttccac
     2341 gtaaacgagc actgtgtttc tgaattttag ttttttcgaa aattctttga tgggctctgg
     2401 aacttctgga agtctgctca cgagcagagt gatgggcata gaggaaacta catgatcata
     2461 tttctctgtg cgtccgcctt ctgtttcaat ggcagttgct tttccgtttt ctgtgatgac
     2521 tctctgcaca ggagtcttga gcattacgtt actgcctttg cttcgaatga aatctgccat
     2581 gcgttcgtag atcattccag tgccgcctga cgggtatgca aactggtcca cgagagtttt
     2641 atgtttgttt
//
LOCUS       DAORFF010000551         2643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_27473_length_2643_cov_3.704791, whole genome shotgun sequence.
ACCESSION   DAORFF010000551 DAORFF010000000
VERSION     DAORFF010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27473_length_2643_cov_3.704791"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..612)
                     /locus_tag="PKK76_17215"
     CDS             complement(169..612)
                     /locus_tag="PKK76_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70273.1"
                     /translation="MGQDKCKEDPVVGTWIALTTHGERKQHLTLRIDENKAGKLRGLL
                     LNLSYSPNLNPSQPPSHCENPDHIIRFKLPGDGTLSGNDFFFRTIELTGTERICMDPT
                     QPDWNYEMDRFKGRLDKKAGTITVIWDDDVYVIHKPIIFRRVSCT"
     gene            complement(692..2503)
                     /locus_tag="PKK76_17220"
     CDS             complement(692..2503)
                     /locus_tag="PKK76_17220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNL70274.1"
                     /translation="MKPFQSFALSAILTAFVYSIGCNPAQSRVPGEFSWTQFDEILRF
                     ARQNYIDPDNIKDNRAYVGAAESALRSLPNPLILYPRKYYQDLRAKQPEDKKIPGNIV
                     EIEKGAAYVVLAPDYSAWDKKQDALAAKERERRKKISSKQRQEEVEKQRKEIMAEQET
                     LEKAWNDVGFGRKDFETVIAWIEKNKGQYSSLPPSYKGENPYRDEPFGIQHVFFASAN
                     GFLQTMDPHSAVMDMESWQKILKESEDSSFEGIGAMLRGGGNQEVVVETPLPGSPAHN
                     AGIRSGDVIVKVDGNSIENMVLGEVVKRIRGPKETFVTLEIERKTEVANLIIRIKRGV
                     IVQKSVSSSYIEDGKIGVIKLSSFLFSEKRPTELIRHEYESLVKKAGGRLGGLVLDLR
                     GNPGGDLDEAIRIAGLFVPENRVVVQIKRRGDKEERRADESRMIPADLPMIVLINAGS
                     ASASEIVASALMDHNTALVLGERSFGKATVQSLQPRGKILIKLTTARYYAPNGYTIQV
                     YGVQPDIAVSEEEDNGFPPRFREEDMWKHLPELTARDRNPAREAWIQKIKAAVGNNEK
                     AEQEIKLHKGDALKPDYMMLRALSYFNALKQQPSPTE"
BASE COUNT          552 a          664 c          675 g          752 t
ORIGIN      
        1 aaccaccgtc acgctgagtc ctgctctggc tgcgatattt gcggcgatca ggcccgcgag
       61 gcctccgcct attactattg aatctttcat tgtttcctcc cgcggtcgaa ccgcttcaaa
      121 ggggagacgg ggggcaggtg tggtttgtga cagagggtct gaattttttt acgtacagga
      181 aacgcgccta aagatgatgg gtttgtggat cacatagacg tcatcgtccc agataactgt
      241 gatcgtaccc gccttcttgt ccagtctgcc cttgaatcga tccatttcat agttccagtc
      301 tggctgtgtt ggatccatgc atatacgttc ggtccctgtt agttcgattg tgcgaaagaa
      361 gaaatcattg ccgcttaacg ttccatcccc cggcagcttg aagcgaatga tatgatccgg
      421 attctcgcag tgtgaaggcg gttgggatgg gttcagattg ggcgaatagc ttagattcaa
      481 gagcaggccg cgcaatttac ccgctttgtt ttcgtcgatg cgaagggtga ggtgctgctt
      541 gcgttctccg tgcgttgtca gtgcgatcca ggtcccgaca accggatctt ctttacactt
      601 gtcctgaccc acaactgtgg gcaggacacg caccgggaac aaaaatagaa ctacgggcag
      661 aacaaatagg accgagaatg ccgtccgggc attactcggt cgggctcggt tgttgtttga
      721 gcgcattgaa ataactcagc gcgcgcagca tcatataatc tggtttgagc gcatcgcctt
      781 tgtgcagttt gatttcttgt tctgccttct cgttgtttcc aaccgcggct ttgatcttct
      841 gaatccaggc ttctcgcgca ggattgcggt cgcgcgccgt gagttcagga agatgcttcc
      901 acatatcttc ttcgcggaaa cgtggtggaa acccgttgtc ctcttcttct gagactgcga
      961 tgtctggctg cactccgtac acctggatcg tgtaaccatt cggagcatag tagcgagcag
     1021 tcgtgagttt gatcaggatc ttgccgcggg gttgcagact ctgtacggtt gcctttccga
     1081 aacttctttc tccgagcaca agagctgtgt tgtggtccat gagagcggat gccacaatct
     1141 cagaagcaga ggctgagcct gcattgatca gcacgatcat gggaaggtca gccgggatca
     1201 tgcgagactc atctgcgcgt ctttcttctt tgtctccgcg ccttttgatc tgcaccacca
     1261 ctctgttttc cggaacaaag agtccggcga ttcgaatcgc ttcatccaga tctccgcctg
     1321 gatttccacg cagatcaaga accagtccgc ccagtctgcc gcctgctttt ttcacaagtg
     1381 attcgtattc atggcgaatc agttctgtag gacgtttctc agagaaaaga aatgagctga
     1441 gcttgatgac cccaatcttt ccatcttcta tataagaaga gcttactgat ttttgcacga
     1501 tcactccgcg cttgatgcga atgatcagat tggccacttc cgtcttgcgt tcaatttcaa
     1561 gagtgacgaa agtctctttt ggtccgcgga ttcgtttgac tacttctcca aggaccatgt
     1621 tctcgatgga gttgccgtct acttttacga ttacgtctcc ggagcgaatg cctgcgttgt
     1681 gagcgggtga tcccggcaga ggagtctcta ctactacttc ctgatttccg ccgccgcgaa
     1741 gcatggcgcc aattccttca aaggaagaat cttcagactc ttttagaatc ttctgccatg
     1801 attccatatc catcacagca ctgtgcggat ccatggtttg cagaaatcca ttggcagatg
     1861 cgaaaaacac atgctggatt ccaaaaggtt catcgcggta aggattttct cccttgtagc
     1921 tgggaggaag actgctgtac tggcctttgt tcttttcgat ccaggcaatc actgtttcga
     1981 aatcttttct accaaatcca acatcgttcc aggctttctc cagagtttcc tgttctgcca
     2041 tgatttcttt gcgttgtttt tctacttctt cctggcgttg tttggaagag atcttcttgc
     2101 ggcgctccct ttcctttgca gcgagcgcat cctgtttctt atcccaggca gaatagtcgg
     2161 gggccagaac aacgtaagcg gctccttttt ctatttcaac aatgttgcca ggaatctttt
     2221 tgtcctccgg ctgctttgct cggaggtcct ggtagtattt gcgtggataa aggatgagag
     2281 gattgggcag agaacgaagt gccgattctg cagcacccac atacgcgcgg ttgtctttga
     2341 tattgtctgg atcaatgtag ttctggcgcg cgaagcgcag tatctcatcg aattgagtcc
     2401 aggagaactc tcctggaacg cggctctgcg ccggattgca gccgatacta tagacaaagg
     2461 cggtcagtat ggcagacagc gcaaaggact ggaagggttt cacgaaaaat aacctcaggg
     2521 tatacacggt tttgggatcg ttcatgcagg gtggttctct tgcgtcgatt cttttcattc
     2581 ttttctgtgg atgcagtacc gcgcctgttc aggatcggcc agtccatgat cgtttagacg
     2641 cat
//
LOCUS       DAORFF010000552         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_27532_length_2640_cov_4.063443, whole genome shotgun sequence.
ACCESSION   DAORFF010000552 DAORFF010000000
VERSION     DAORFF010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27532_length_2640_cov_4.063443"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PKK76_17225"
     CDS             complement(<1..224)
                     /locus_tag="PKK76_17225"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNL70275.1"
                     /translation="MRFLVLIVLAACSVPTRSGPSYLGVLYQESREGVEVVQIYPGSP
                     ARTAGLAIGDTIVAVDGQRVGRSSSLRAAI"
     gene            937..2448
                     /locus_tag="PKK76_17230"
     CDS             937..2448
                     /locus_tag="PKK76_17230"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNL70276.1"
                     /translation="MSLPESVLIANRGEIAVRIVRTCRKLGIRTIVSYSSADRNSLAV
                     RIADEAIEIGPPDSRSSYLNIEAIIAACKKTRAAAVHPGYGFLSENAEFASRLAAEGI
                     QFIGPSPDTIRSMGDKISARKLMQDKGVPVVPGYNGDDQSPGRLKEAAMSIGLPALIK
                     ASAGGGGRGMRVVTREEEFIPALEAAKREALGAFGDDRVFVEKYVSSPRHIEVQVLGD
                     SHGKVVALFERECSLQRRHQKIVEESPSPALTELLREQIMKAGVLAAEAVSYVNAGTV
                     EFIYSDTERTFYFLEMNTRLQVEHPVTEMVTGIDLVEQQLRVAGGLPFSFDPASLSQT
                     GHAIEVRLYAEDPEKNFLPATGKVHLFETPADVRVDSGIESGDEVTIHYDPMISKIIS
                     HGVNRAEAIDKMTKALDETVLFGPNNNLDFLRSIVRHPVFRSGKITTGFIPEHFPSFR
                     RTSSPDTVELARASASVLTYLGGKQISGSELNLAQTGGDLLPWIQIEGFRLWN"
     gene            2439..>2640
                     /locus_tag="PKK76_17235"
     CDS             2439..>2640
                     /locus_tag="PKK76_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNL70277.1"
                     /translation="MELGSKVEVTREEVNGGTIVHVKFDQPETRNSLTLEMGLSFHRV
                     MHALAGEHPLPRACILSGKNGVF"
BASE COUNT          715 a          660 c          665 g          600 t
ORIGIN      
        1 atgatggcgg cgcgcaaaga agaagatcta cccactctct ggccgtccac agcgacaata
       61 gtgtcaccaa tggcaagacc agccgtgcgg gccgggcttc ccggatagat ttgcaccact
      121 tctacacctt cccggctttc ctgatagaga acgcccagat aagacggacc gctcctggtg
      181 ggcacggagc aagcagccag gacaattaga accaggaatc tcacaatgaa ttatcggaga
      241 tgggcctgcc tgattgcaag acctaaacca gtgcaattga ttttttaacg agaaaaacgg
      301 ggaaacaacc cacgctgaaa aagccgccgg ggctcatcca acgtggtcaa gagtctagcg
      361 gtcccgcttg gagcgtttgg accaattctt acgatatgct tgatctgaaa cctgcttggg
      421 cttcagacca tacttgccag caatctgacc aggctcctgg cctgaacgat atgctttttc
      481 gatgctcgcc ttttgcgagg acgaaaggtc tgtccaattt ttcttcatgc attgcctcac
      541 tatctatggg gatgttcttt agaagcgcta atttatccaa ccggtcaata acaaaaatgc
      601 gaaggagtgc gcgaagatga aaaaactttt cactttcgca cgcgtcacga aaatgaacga
      661 catgtttttg gatatgaaca acactttcgc aaaaacgcgc gaaaatcgaa agcgaatgtg
      721 cggagaaaac ttgtgcgaaa atgtgcgaag acttcattcg caacatgtgt gcgagaagag
      781 acaagatgcg aacgcattcg agtcatcatg cgaagctaca gcaagcggaa ccttcatcgt
      841 tggctcgagc atgaagtttc aaaaccaaaa aactggcatg cgaaatgcac aagcaattgc
      901 aattgacatt gccccggtcg cagcctttct acgcccatga gtctacctga atcagttctc
      961 attgcgaatc gcggcgaaat cgcggtgcgc atcgtgcgaa cgtgccgcaa gctgggcatt
     1021 cgcacaatag tttcatattc aagtgctgat cgtaactctc tggcagttcg aattgcagat
     1081 gaggctattg agattgggcc tcccgattcg cgtagctcat acttgaatat cgaagcaatt
     1141 atcgcggctt gcaagaagac tagagctgcc gcagttcatc ctggatatgg attcctgtcc
     1201 gagaatgctg aatttgcaag cagactggct gcagaaggca ttcaattcat tggtccgtca
     1261 ccggatacaa ttcgatccat gggagacaaa atctccgctc gcaaactcat gcaagacaaa
     1321 ggggttcccg ttgtaccagg atacaatgga gatgaccaga gtcctggacg actcaaagaa
     1381 gcagccatgt cgatcgggct tcccgctctc atcaaggctt ccgccggggg aggaggaagg
     1441 gggatgcgcg tggtcacccg agaagaagag tttatcccgg cgctcgaagc ggcaaaacgg
     1501 gaagcactcg gagcattcgg cgacgatcga gtctttgttg aaaaatacgt ctccagtccc
     1561 aggcacatcg aagtacaggt gttaggcgat tcgcatggca aagtggtcgc tctctttgaa
     1621 agagaatgct ctctgcaaag aaggcatcag aagatcgtag aagaaagccc gagccctgcc
     1681 ctcaccgaac ttctaagaga acagatcatg aaagcgggcg ttctggcagc agaagcagtg
     1741 tcatatgtaa atgccggaac cgttgaattc atctattcag acacagaacg cacattctat
     1801 ttcctcgaaa tgaataccag gctccaggtg gagcatcccg tcactgaaat ggtcactgga
     1861 atcgatctgg tggaacaaca actccgtgtg gcaggcgggc tacctttctc ctttgatccc
     1921 gcttctttga gccagacggg gcatgccata gaggtcagac tgtatgcgga ggatccggag
     1981 aagaatttcc ttccggctac aggcaaagtg cacctttttg agactccggc tgacgttcga
     2041 gtcgattcgg gcattgagtc aggagacgaa gtcaccatcc actatgatcc catgatttca
     2101 aagatcatat cccatggcgt caatcgcgca gaagcgatcg acaaaatgac aaaggctcta
     2161 gacgaaacgg ttttgtttgg gccaaacaac aacctggact tcttgcgttc gattgtaagg
     2221 caccctgtat ttcggtcggg caaaatcacg actggattca tccccgagca cttcccttct
     2281 tttagaagaa cttcttctcc agacacagtt gagcttgccc gggcatccgc ctctgtttta
     2341 acttaccttg gcggcaaaca aatctccgga tcagaactca atctggctca gaccggcgga
     2401 gatctgctac cgtggattca aatcgagggg ttccggcttt ggaattaggc tcaaaagtag
     2461 aagtaacaag agaagaagtg aatggcggaa ctatcgttca tgtaaagttc gatcagccag
     2521 agaccaggaa ttcgctgact ctggaaatgg gtctttcctt tcaccgcgtc atgcatgcgc
     2581 ttgcaggcga acatcctctt ccacgggctt gcattctatc tggtaaaaac ggagtcttct
//
LOCUS       DAORFF010000553         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_27534_length_2640_cov_3.665764, whole genome shotgun sequence.
ACCESSION   DAORFF010000553 DAORFF010000000
VERSION     DAORFF010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27534_length_2640_cov_3.665764"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PKK76_17240"
     CDS             <1..414
                     /locus_tag="PKK76_17240"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL70278.1"
                     /translation="LGLGLLTAIGYPLYPPGQLGGPILAAVLGGFFVASIILFESLVA
                     DMAEAERVRSGEAREGIFFGFWKMANKAARALGLALSGVALSGIGFSNTVTPGPEIAW
                     KIALLFGPGVGACFIAAAVIFLGVQEPEKPVQNPV"
     gene            472..1731
                     /locus_tag="PKK76_17245"
     CDS             472..1731
                     /locus_tag="PKK76_17245"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNL70279.1"
                     /translation="MKRTLAIVGSGISGMGALYALRDEFAITLYEKADRIGGHTHTVT
                     VEDPSGPLPVDTGFIVYNTTTYPLLTRLFEELKIKTEWSDMSFGVQNLDTGLEWAGHN
                     LGSVFAQKKNLLRPAFWRMVLEILRFNRTALRRAQENSEEISLGDFLRNDRYSDYFID
                     NYIVPMGSAVWSTPARNMLDFPARTFIEFFSNHGFLEVERRVRWRTVSGGSHSYIKAI
                     LDEGKPEIQTGQPVTSVRRTASGKVEVTTPLEKREFDFVLMASHANETLSMLADPTHA
                     ETELLSHFHYQKNEAVLHFDESVLPSAPQCHAAWNFRTKSGVQAIAYSMKRLQNIKSD
                     RHYIVSLGEFERIDEKKIIRSMTYEHPLFDLKTAQAQKSLHLLNETGPIFFSGSYFRY
                     GFHEDGLWSASLAARRILDVSRQLVHV"
     gene            1703..2518
                     /locus_tag="PKK76_17250"
     CDS             1703..2518
                     /locus_tag="PKK76_17250"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNL70280.1"
                     /translation="MFRGNSCMYEGRVSHVRQKPVRNSFTYGLYMFAIDVDELPRLDR
                     SMISVNRRGVFSFYDQDHLKFIPGDQAAPIAGHSDLRVRLEKYLATVRPGFVPSRILL
                     VTNLRVFGYVFNPVSFYFLFDDSSHEETVRAVLVEVNNTYLEQKAFLIEDSSLEKVEQ
                     KLFYVSPFVSYDDTFHFRLTPPEDSLTVMIDSKRENHVEVRSVLKANRVPMSRWELVR
                     LSIRYPLQTIRVIFLIHWQAMRLYLRGVPFFQKKATDDLIRMHAQRNHPGGTS"
BASE COUNT          674 a          669 c          633 g          664 t
ORIGIN      
        1 ctgggccttg gccttttgac tgccataggc tatccacttt atcctccggg tcagctgggt
       61 ggtccaattc tagccgctgt gctcggcgga ttctttgtcg catcgatcat ccttttcgag
      121 tctcttgtag cggacatggc ggaagcagag agagtgcgtt ctggtgaggc cagagaaggg
      181 atctttttcg gcttctggaa aatggctaac aaagccgcaa gagcgcttgg tcttgcactg
      241 tccggggtgg ctctcagcgg aattggtttt tcaaacacag taactcccgg gccagagatt
      301 gcctggaaaa ttgcacttct attcggcccg ggcgtcggag cctgttttat agcagcagct
      361 gtcattttcc ttggtgtcca ggagccagaa aaacccgttc aaaatcctgt ctaatgtttt
      421 caaaaaacat tggacgaaat tcaagaacag gatttcctta tccagtagca catgaaacgc
      481 acgctggcta ttgttggttc ggggatctcg ggaatgggag cgctgtatgc gctgcgagat
      541 gaattcgcca tcaccctgta tgaaaaggca gatcgaattg gcggacacac tcatactgtg
      601 acagtagagg atccgtccgg tccgctacct gtggacactg gctttatagt atataacaca
      661 accacgtacc ccctcctgac tcgactcttc gaagaactga aaatcaaaac agaatggagc
      721 gatatgtcct ttggtgtgca gaacctggac acaggacttg aatgggcggg tcacaatctg
      781 ggaagcgtat tcgcacagaa gaaaaatctg cttcggccgg ctttctggag aatggttcta
      841 gaaattctca gattcaaccg cacagcactc agacgcgccc aggaaaactc cgaggaaatt
      901 tctcttggcg atttcctcag aaatgaccgt tacagcgact acttcataga caactacatt
      961 gtccccatgg gctctgctgt gtggtccact cccgcgagaa acatgctgga tttccctgcg
     1021 agaacattca tcgagttttt ttcgaatcac ggatttcttg aagtagaaag acgcgttcgc
     1081 tggcgcactg tatcaggtgg atcgcattcc tatatcaagg caatcctgga tgagggaaag
     1141 ccagaaatcc aaacagggca acctgtaaca tctgtcaggc gcaccgcgag tgggaaagta
     1201 gaagtaacaa cgccacttga aaagcgagag tttgatttcg ttctcatggc gtctcacgcg
     1261 aacgaaactc tttctatgct agcagatcca acgcatgcag aaactgagct tctgtctcac
     1321 tttcactacc aaaaaaatga agcagtgctg cattttgacg aatccgtctt accttctgct
     1381 ccgcagtgtc atgcggcgtg gaatttccgc actaaatctg gagtgcaggc catcgcatat
     1441 tcaatgaagc gcctgcaaaa tatcaagtct gatcgccact acattgtttc cctgggagag
     1501 ttcgaacgca tagacgaaaa gaaaatcatt cgcagcatga catacgaaca tcctctattc
     1561 gatctgaaaa cggcccaggc acaaaaatcc ctgcacctct tgaatgagac cggacccata
     1621 tttttctccg gcagctattt tcgctacggc ttccatgaag atgggctctg gtctgcgtcc
     1681 ctcgccgcaa ggaggattct cgatgtttcg aggcaactcg tgcatgtatg aaggacgtgt
     1741 atcacacgtc agacagaaac ctgttcgtaa ttcatttacg tacggtctct atatgttcgc
     1801 catagacgtg gatgaactcc ccagactgga tcggtccatg atttcagtga atcgtagagg
     1861 cgttttttcc ttctacgatc aggatcatct aaaattcatt ccaggcgatc aggcagcacc
     1921 tatcgcggga cactcagatc ttcgcgtgag attggagaag tatctggcaa ccgtccggcc
     1981 aggcttcgtg ccttctcgca ttctgctggt caccaatctt cgcgtgtttg gatacgtgtt
     2041 caatccagtg agcttctatt tcctgttcga tgactcttcg cacgaagaaa cagtgagggc
     2101 cgttcttgta gaagtgaaca acacgtatct ggaacaaaaa gcgtttctga tcgaagatag
     2161 ttctctggaa aaagtagaac agaaactatt ctacgtctcc ccttttgtct cctacgacga
     2221 cacgttccat ttccgtctca ctcctccaga agatagtctc acagtgatga tcgactcgaa
     2281 gagagagaac catgtggaag tcagatctgt tctgaaagca aatcgcgtcc ctatgtccag
     2341 gtgggagctg gttcgcctgt cgattcgcta cccgctgcag accattcgcg tgatctttct
     2401 catccactgg caggcaatgc gtctttatct tcgaggtgtt ccctttttcc agaaaaaggc
     2461 cacagacgat ctgatccgaa tgcacgcaca aagaaaccat cctggaggca caagttgaaa
     2521 gcgcttgcaa cgaacatcac gcccgaaatt aaaaaggagc gcacgccgcg gtcagccgcg
     2581 gagcgaatct tctataaata tcttgctcgc gcaaatcgcg gatcactgga gattcaattc
//
LOCUS       DAORFF010000554         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_27543_length_2640_cov_2.860735, whole genome shotgun sequence.
ACCESSION   DAORFF010000554 DAORFF010000000
VERSION     DAORFF010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27543_length_2640_cov_2.860735"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PKK76_17255"
     CDS             complement(<1..436)
                     /locus_tag="PKK76_17255"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNL70281.1"
                     /translation="MKFDHQHKILYEAGYRYIDYRRFIQPVGVRLTLVFITVVLTIIF
                     GFRLFFLGTLVRPLQALLSGVRKVNKGNLAVHVPVGVADEIGFLTESFNGMVSSIRIA
                     REKLERHADELEVKVRERTAELTETLQTVQDLKSQQDGDYFLT"
     gene            complement(530..1840)
                     /locus_tag="PKK76_17260"
     CDS             complement(530..1840)
                     /locus_tag="PKK76_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70282.1"
                     /translation="MQDVFFFNFFVVSILIGAVFYIIVGGFLLTMIPEKSTATRTLGF
                     AYLFSSGLAAAYVFGYSVYHPFGAYHRWLSIPSFLVTHPLFGQLFYHFPRTRYPKVAR
                     TVLTIQVSIGVILTAAFIYTSLSRGAVYSFSGHFWDFDMDDWSKAVALVALLNLVHLI
                     VAAVVHIIVVPEKRLALGLMFFGFMFAILIPTVTNIMSRDGLFPRDLHQTLLVLLSIT
                     GLFTVLIVYLNHTQDKTTFMVKIVGISLVTFMVVMVFITYFSFQDKEDAYDAVRIERT
                     SRLAIEKNYPVDDLAYLVSYDAVSGKIEVLSGKPDAIDYSDTEPEFASAVIYERIRNL
                     NQTEWKSGLEEILKNSPEEFQGYAGFIRSLAVTAPSAQAVLALVDKDSQQFTYRSIKI
                     RELPDKDFRKLLTAYNDKLPPKLASFAEAIRRYLASHPDMDGAK"
     gene            complement(1993..2397)
                     /locus_tag="PKK76_17265"
     CDS             complement(1993..2397)
                     /locus_tag="PKK76_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004777999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HNL70283.1"
                     /translation="MAGYSKKALQSKLGLKPGQIVIFINPPSDYSTHLGALPEGIELK
                     SELSGGFHFIQMFTKHKSELEDRLPFLKSSLVEKGMIWVSWPKKASGVETDLTEDVIR
                     ELAIKNGLVDVKVCAINEVWSGLKLVLRKTPA"
     gene            complement(2407..>2640)
                     /locus_tag="PKK76_17270"
     CDS             complement(2407..>2640)
                     /locus_tag="PKK76_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70284.1"
                     /translation="GKDISTGCLAMGDPAMDELYTMVMDAGMTNVTVVIAPNDLRRRG
                     PVTNMRAAPRWTQDLYKDIKTSLVKFSKTEKIR"
BASE COUNT          732 a          645 c          614 g          649 t
ORIGIN      
        1 aagtaaggaa atagtctccg tcctgctggc tttttagatc ctgaacagtc tgcagtgttt
       61 ctgtgagttc cgcggttctt tcacgcactt ttacttccag ctcatctgca tgacgttcca
      121 acttttcgcg cgcaattcta atagaagaaa ccatcccgtt gaaagactct gttagaaatc
      181 caatttcgtc cgccacacca accggaacgt gtactgccag gttgcctttg tttactttgc
      241 gtactccaga aagaagagcc tgaagcggac gcaccagagt cccgaggaag aaaagtctga
      301 atccgaaaat gatcgtaagt acgactgtaa tgaacacgag agtcaggcga actccaacag
      361 gttgtatgaa acgacgatag tcaatgtacc gatatcctgc ttcgtacaga atcttgtgct
      421 gatgatcgaa cttcatgaaa gcagtgtagt gatgattggc cgcgtctgat cggtagcgtc
      481 tggtagtttc cggatgaaaa ggactgaaga aatgaagaac ctcctgtttt cactttgctc
      541 catccatgtc agggtgagat gccagataac gtcgaatcgc ttctgcaaat gaggccagtt
      601 tcgggggcaa cttatcattg taagcagtca acagtttccg aaagtctttg tccggtagct
      661 cgcgaatctt gatgcttctg taagtgaatt gctgagaatc cttgtccaca agagcaagca
      721 ccgcctgtgc cgacggcgca gtcactgcca gactccgtat gaatccagcg tatccctgga
      781 actcttccgg actgtttttc agtatttctt caagacctga tttccattcg gtttgattga
      841 ggttgcgaat gcgttcgtaa atcactgcac ttgcaaattc cggctccgtg tccgaatagt
      901 cgattgcgtc cggcttcccg gaaaggactt caatttttcc gctaaccgca tcatacgaaa
      961 caagatacgc tagatcatcg actggataat tcttctcaat cgcaaggcgc gacgttcttt
     1021 caatgcgaac ggcgtcgtac gcatcttcct tgtcctggaa tgaaaagtaa gtaatgaaaa
     1081 ccattacaac catgaatgtg accaggctga ttccaacgat cttcaccatg aatgtagtct
     1141 tatcctgggt gtgattcaaa tagacaatca gcactgtaaa caaaccagta atcgacaaaa
     1201 gaacgagaag agtctgatgc agatcgcgcg gaaacaaacc atcgcgactc atgatatttg
     1261 tgacagtagg aatcaaaatc gcaaacatga aaccgaagaa catcaatcca agcgcgagac
     1321 gtttttctgg aaccacgatg atgtgcacga cagccgcaac aatcagatga accaggttca
     1381 agagcgcaac gagcgcaacc gctttgctcc agtcgtccat gtcaaaatcc cagaagtgcc
     1441 cggagaatga atacaccgct ccgcgcgaaa gggatgtata gataaacgct gcagtcagga
     1501 tcactccaat cgacacctgg atcgttagca ctgtgcgcgc cacctttgga taacgagtgc
     1561 gtgggaagtg atagaagagc tgaccaaaca acggatgagt tacaaggaag gaaggaatgc
     1621 tcagccatct gtgatacgca ccaaatggat gatagacgga gtatccaaag acgtatgcag
     1681 cggccagacc gcttgagaat aaataggcaa atcccagagt gcgagtggcg gtggacttct
     1741 caggaatcat agtcagcaga aatccaccga cgatgatata gaaaactgcc ccgatcaaaa
     1801 tagaaacaac gaagaagttg aaaaagaaga cgtcttgcat ctagtcgctc ctcggaatgt
     1861 gcgaagccta taacctatct tctacacgag tagcgtcgag ccgaaattaa aattcgtgcg
     1921 aaggcgaaac ctcgcacagg caagcgagct aacaatgttc acttcgagca gtgccgaatc
     1981 gtctaactga atttaggccg gggttttacg aaggaccagc ttcaagcccg accacacttc
     2041 attgatggcg cagaccttga catccacgag gccattcttg attgcaagtt cacgaatcac
     2101 atcttccgtc agatccgttt ccactccgga agctttcttg ggccaggaaa cccagatcat
     2161 tcctttttca accagactgg atttcaaaaa gggaagacgg tcttccagtt ccgacttgtg
     2221 ctttgtgaac atttggatga aatgaaaacc accgctgagt tcacttttca gttcaatccc
     2281 ctcggggagc gcgcccagat gagtggaata gtccgagggc ggattgataa atatgacgat
     2341 ttgcccgggt tttaggccca gttttgactg gagtgctttt ttagaatatc ctgccactga
     2401 attcccttat cggatctttt ccgttttact aaatttgacg agagaagttt taatatcttt
     2461 gtacagatcc tgcgtccacc gaggagccgc gcgcatattc gtaacgggcc ctctccttcg
     2521 caagtcgttt ggggcgatca ccacggttac gtttgtcatt cctgcgtcca tcaccattgt
     2581 atagagttca tccatcgcag ggtcccccat cgcaaggcat ccagtggaaa tatcttttcc
//
LOCUS       DAORFF010000555         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_27623_length_2635_cov_4.107752, whole genome shotgun sequence.
ACCESSION   DAORFF010000555 DAORFF010000000
VERSION     DAORFF010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27623_length_2635_cov_4.107752"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /locus_tag="PKK76_17275"
     CDS             complement(<1..1436)
                     /locus_tag="PKK76_17275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNL70285.1"
                     /translation="MNILSIDRVSKHIGDRSLFENLTFGIADDDRLGLIGPNGAGKTT
                     LLRILSGDSEPDDGRVAKSTQLQIANLPQIPVFQPDLSIRQALLSPISISNKRVTEDM
                     EREMVGILRELGMTDLDLRMDSLSGGMIKKVALAQTLALDADLLILDEPTNHLDAWTN
                     LWLEDVLRAWKKALLVITHDRYFLENVTNRILELEDGKLYQYEGNYSYYLEKKAEREA
                     SEARTQAKQSRLLKSELEWLRRQPKARGTKQKARVDRIEDLAANQRGGQERAFAFDSS
                     GTDSKNRILEVKGLGMSYGGRSLFSGFEHTFRSRERLGIIGPNGCGKSTFFNILMGKI
                     EPEAGTLKKGLHTKFGYFDQTSRDLDPNAKVLEYVKKNAGEIVGRADGSMTEATRLLD
                     YFGFSSRMQQGPIGKLSGGERRRLHLILMLMASPNFLILDEPTNDLDIQTLVLLEEFL
                     SDFQGCVLVASHDRYFLDRVAASLLVYE"
     gene            1571..>2635
                     /locus_tag="PKK76_17280"
     CDS             1571..>2635
                     /locus_tag="PKK76_17280"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNL70286.1"
                     /translation="MAEKIYIFDTTLRDGEQSAGVAFTGEEKLEIAFQLERLKVDIIE
                     AGFPCTSPGDLKAVQLISGEIKNATICALARAVPRDIDLAWEAVQHAKDPRIHVFINT
                     SDIQIAHQLKKGRDEVLIQAEAMVRHAAKYTSNVEFSPMDATRSDREFLYLMIERCID
                     AGATTINVPDSVGYAIPEELGGLFRSIMENVKNIHKCRISFHGQNDLGLCTANSLSAI
                     QNGVRQIECTINGIGERAGNTSLEEVVMAIKTRKDFLQFYTDIQTEEIYRASKLVEGL
                     SGMPVQWNKAIVGKNAFRHGSGIHQDGILKLRETWEIMNPAEIGIPKGTELVMGKLSG
                     RHAFKVHLEEMGYHLSEAETE"
BASE COUNT          656 a          640 c          674 g          665 t
ORIGIN      
        1 ttttcgtata caagaagaga agcggcgacg cgatctagga agtagcggtc gtgagatgca
       61 accagaacgc agccctggaa atcagacaag aactcttcca atagaacgag agtctggatg
      121 tccaggtcat tggttggttc atcgagtatg agaaagttgg gagaagccat cagcatcaag
      181 atcaaatgca atcttctacg ttctccgcct gacagtttgc caatggggcc ttgctgcatg
      241 cgcgaagaga atccaaagta gtccaggaga cgcgtggctt ccgtcatgct tccgtctgcg
      301 cgtcccacaa tttcgcctgc gttctttttt acgtactcca gtactttcgc gttcggatcg
      361 agatctcgtg atgtctgatc aaagtaacca aactttgtgt gaagtccttt tttaagagtg
      421 ccggcttctg gttcgatctt gcccatgaga atattaaaga aagtagactt tccgcaacca
      481 ttcggtccaa tgattccaag tctctcgcgc gagcgaaacg tatgctcaaa tcccgagaac
      541 aggctgcggc cgccgtaaga cattcccagg cccttcactt ctagaattct gttcttgctg
      601 tctgttccgc ttgagtcgaa tgcgaacgca cgctcctgtc caccgcgctg atttgcagcc
      661 agatcttcga ttcgatccac gcgcgctttt tgtttggttc cgcgcgcttt tggctgtctt
      721 ctcagccatt ccagttccga tttgagaaga cgtgattgct ttgcctgcgt gcgcgcttcg
      781 ctcgcttccc tttccgcttt cttttccagg tagtaagagt agtttccttc atactgataa
      841 agctttccat cttccagttc cagaatgcga ttggttacgt tttcaaggaa gtatctatcg
      901 tgagtgatga cgaggagtgc ttttttccag gccctgagta catcttctaa ccacagattg
      961 gtccacgcgt ccaggtggtt ggttggttca tccagaatca gcaaatctgc gtcgagtgct
     1021 agagtctgtg cgagagccac tttcttgatc attccgccgg aaagtgaatc catgcgaaga
     1081 tccaggtccg tcatacccag ttcgcgcagg atcccaacca tttcgcgttc catatcttct
     1141 gtgactcgct tgtttgaaat ggagatggga gagagaagtg cctggcgaat cgacaggtct
     1201 ggctggaaga ccggaatctg cgggaggttt gcaatttgca gctgtgtgga tttcgcaacg
     1261 cggccatcgt ctggttctga gtcgccggag agaatgcgaa gcagagtcgt cttgcctgct
     1321 ccgtttggcc caatgaggcc cagtctgtcg tcgtctgcga tcccgaacgt caggttctca
     1381 aacaaagagc gatcgcctat gtgttttgaa acgcgatcta tagacagaat gttcatctag
     1441 ttcagtggct ccgatctgca aagcgcccag ctggaaaaag cgcttcctct gccagtgaaa
     1501 ggcgagcccg tggcatggca aaaacaaatg tcgcttgccc ttttcttgcc ggacatttcc
     1561 ctggaatggc atggcagaaa aaatctatat ctttgataca actctccgcg atggagagca
     1621 gtccgcagga gtggcattca ccggcgaaga aaagcttgag attgcgtttc aactggaacg
     1681 cctcaaagta gatatcatcg aggctggctt tccgtgcact tctcctggag atttgaaagc
     1741 ggttcagctc atttcagggg aaatcaagaa tgcaaccatc tgtgccctgg cccgggctgt
     1801 tccgcgtgac attgaccttg cctgggaagc agtgcagcat gcaaaggatc cacgcattca
     1861 cgtgttcatc aatacaagtg acattcaaat cgctcaccag ctcaaaaaag gtcgcgatga
     1921 agtactgatc caggcagagg ccatggttcg ccatgctgcg aagtacacat ccaacgttga
     1981 attcagtcca atggatgcaa ccaggtcaga tcgagagttt ctgtatctga tgatcgaaag
     2041 atgcatcgac gcgggtgcga caaccatcaa cgttccggat tctgttggtt acgcgatccc
     2101 agaagaactc ggcggccttt ttagatccat catggaaaac gtgaagaaca ttcacaaatg
     2161 cagaatcagt tttcacggtc aaaacgatct tgggctttgc actgcgaatt ctctctccgc
     2221 cattcagaat ggagtgcgcc agattgaatg caccatcaac ggaattggag aaagagcagg
     2281 gaacacttct ctggaagaag tggtcatggc catcaagacg cgaaaggatt ttcttcagtt
     2341 ctacacagac attcaaacag aagagattta tcgggcgagc aaactggtag aagggctttc
     2401 cggaatgccg gttcaatgga ataaagcaat cgttggtaag aacgcgtttc gtcatggaag
     2461 cggaattcac caggatggaa ttttaaaact gcgcgaaacc tgggaaatca tgaacccggc
     2521 ggaaatcggt attcccaaag gaaccgagct tgtaatggga aagctttcag gtcgtcatgc
     2581 gttcaaggtg catctggaag aaatgggata tcatctgagc gaagcggaaa cggaa
//
LOCUS       DAORFF010000556         2632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_27681_length_2632_cov_3.232829, whole genome shotgun sequence.
ACCESSION   DAORFF010000556 DAORFF010000000
VERSION     DAORFF010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27681_length_2632_cov_3.232829"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PKK76_17285"
     CDS             complement(<1..495)
                     /locus_tag="PKK76_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNL70287.1"
                     /translation="MLFLKKNAPRILVGGMFLLLAAQLIWWLTFFRLKQKRFEQLEDA
                     LDEQRIALMRASRGSFPDIVCNDGKCAVRPEILAERARRHAREMWMLLSETMFVLSVI
                     GYGSWLVFRSVQKEKQLARERITFLNSIAHELKTPIAGTQLALQTLQKRSLSPKQRSD
                     LLNAG"
     gene            complement(497..1783)
                     /locus_tag="PKK76_17290"
     CDS             complement(497..1783)
                     /locus_tag="PKK76_17290"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNL70288.1"
                     /translation="MNQTNSQSLYARATRVIPGGVNSPVRAFRSVGGTPIYMESATGS
                     RLRDADGNVYVDFCNSWGPLITGHRHPAILSAIRSQMLRSLTFGTPCEQEVLLAEHVI
                     QLLKRSVPDCRIEKIRFMSSGTEAVMTAIRLARGFTNRSKILKFDGCYHGHSDSLLAK
                     AGSGMATLGVPDSAGVPASFTTETIVIPLDDEAALENAFVQHGRDIAAILIEPVPANN
                     GLLLQRKEFLQKLKYMAVAGGALLIFDEVISGFRVAPGGAAQYYGICPDLVTYGKIIG
                     GGMPVGALAGRNDVMDRLAPDGPVYQAGTLSGNPVAMSAGLAQLKLLEQPAYEHLETL
                     GRNLETAFNAASLSWKMVRIGSIFWFYQGQEPPRRADKIGRDSMKVYAEFHRYCLERG
                     MYLAPSGYEVGFLSTPMTEQEVSRFLGLAVDFFKSR"
     gene            complement(1780..>2632)
                     /locus_tag="PKK76_17295"
     CDS             complement(1780..>2632)
                     /locus_tag="PKK76_17295"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNL70289.1"
                     /translation="QKGKTYFQMHMFLGEEAIQNRRKKSYQVTRCADTPERLSKAMLE
                     DVLYSHAIVVTGRPARCAELKQKFPHIHSLPLIDTQILPLPRDLEPSIRKEGGLVAAF
                     FSESGVAAASSIRALREDNVQFAAVSHRTAEAVKNTFGRSAIVGTATGKDLAHAVIEK
                     TYGPVFAFRSQDGREDFMEVLGAAGRPYRDVPLYKTTHLPVDRSQLDSLPARAAVIFA
                     SPSAFLSWIKAAGGGSSASAYHLERGLRLCAIGNTTADAMLEMNCVPYAISEHPDLDA
                     FIQELMQ"
BASE COUNT          598 a          690 c          689 g          655 t
ORIGIN      
        1 tccagcgttg agcaagtccg atctctgttt tggagaaaga gatctctttt gtaaagtctg
       61 aagagcgagc tgtgtgccgg cgatgggagt cttaagctca tgtgcgattg aattcaggaa
      121 cgttatgcgt tcacgtgcca gttgtttttc tttttgaacg gatctgaaca ctagccagga
      181 tccatacccg ataacgctca gtacgaacat tgtctcagac aaaagcatcc acatctcgcg
      241 cgcatggcgg cgcgctcttt ctgccagaat ctctggtctc accgcgcatt ttccgtcgtt
      301 gcatactata tcagggaaag agcccctgga tgcacgcatg agcgcaatgc gttgctcatc
      361 gagtgcatct tccagctgtt caaagcgctt ttgtttgagt cgaaagaagg tgagccacca
      421 gatcagctgt gctgcgagca atagaaacat gccgccgact agaatccgcg gcgcgttctt
      481 ctttagaaaa agcatctcaa cgacttttga aaaagtccac agcaagtccc aggaatctag
      541 acacttcctg ttccgtcatg ggagtgctca aaaagccaac ttcgtatccg gagggcgcca
      601 gatacattcc gcgttccagg cagtagcggt ggaattcagc atacactttc atggagtcgc
      661 gtccaatttt gtctgcgcgc ctgggtggct cctgtccctg gtagaaccag aaaatagaac
      721 caatgcgcac catcttccag gagagagagg cagcattgaa cgcagtttcc agatttcgac
      781 cgagtgtttc tagatgttcg taagccggct gttctagaag tttcagctgt gcgaggcccg
      841 cagacatagc cactggattg ccggataacg ttcctgcctg gtaaacggga ccatccggtg
      901 ccaggcgatc catcacatca tttcttcctg ccagggcgcc cacaggcatc ccgccaccaa
      961 tgatcttgcc gtaggtaacc aggtccgggc aaattccata gtactgtgct gctccacccg
     1021 gggccacgcg aaagccgctg atgacttcgt caaagatcaa aagtgctcca cctgcgaccg
     1081 ccatgtattt cagtttctga agaaactctt ttctctgaag caacaaacca ttgttagctg
     1141 gaacaggttc aatgagaatg gcagcaatgt ctcgaccatg ctgcacgaaa gcgttttcaa
     1201 gagccgcttc gtcatcgagt ggaatgacaa tggtctctgt tgtaaaagaa gcaggaacac
     1261 cggcggaatc aggtactccc agagtggcca tcccggatcc agctttcgca agcagactgt
     1321 ccgagtggcc gtggtagcat ccgtcgaact tcagtatctt gctgcgattt gtaaaacccc
     1381 tggccaggcg aatcgcagtc atgaccgctt ctgtcccgga actcatgaaa cgtatctttt
     1441 caattctgca gtcaggaacg ctccttttca atagctggat gacatgttct gccaggagca
     1501 cttcctgttc gcatggagtt ccaaatgtta aagagcgaag catctggctg cggatggcgg
     1561 agagaatggc cggatgtctg tgacctgtga tcagaggtcc ccaggaattg cagaagtcta
     1621 cgtaaacgtt tccgtccgca tctcgcagtc gcgagccagt ggcactttcc atatagatgg
     1681 gcgtgccgcc cacggatcga aacgcacgta ctggactgtt gactccaccc gggatcacgc
     1741 gcgtggcgcg tgcgtataga ctttgtgagt ttgtctgatt cattgcatta gctcctgtat
     1801 aaatgcgtca agatccggat gctccgaaat ggcgtaaggc acgcaattca tttctaacat
     1861 tgcatccgca gtagtatttc caatggcgca cagcctcagg ccgcgttcga ggtggtaggc
     1921 ggaggcagaa gagccacctc ccgccgcttt tatccaggaa aggaatgcag aaggactggc
     1981 gaaaatgacg gcagcgcgag caggcagcga gtccagttga gatcggtcta ctggcaggtg
     2041 ggtggttttg tagagaggga cgtcccggta gggtctgcct gcagctccca gtacttccat
     2101 gaagtcttct cttccgtcct gggaacgaaa cgcgaagact ggtccgtacg ttttttctat
     2161 gactgcatgc gccaggtcct ttcctgtggc agttcctaca atcgcacttc tgccgaatgt
     2221 gttcttcact gcctctgccg ttctgtggct tacggcggcg aattgaacgt tgtcttcgcg
     2281 cagtgctcgg atgcttgagg ctgctgccac tccactttcg ctgaaaaatg cagccacaag
     2341 gccgccttct tttctaatgg atggttccag gtctcgcgga agaggaagaa tctgagtgtc
     2401 gatcaggggc aggctgtgaa tgtgagggaa tttctgtttt agctccgcgc atctagccgg
     2461 tctgccggta accacaatcg catgcgagta gagaacatct tccagcattg ctttgctcag
     2521 tcgttctgga gtatctgcgc atcttgtaac ctggtaggat ttttttctcc gattctggat
     2581 ggcctcttcc cccagaaaca tatgcatttg aaaatacgtc ttgcccttct gc
//
LOCUS       DAORFF010000557         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_27741_length_2629_cov_3.993007, whole genome shotgun sequence.
ACCESSION   DAORFF010000557 DAORFF010000000
VERSION     DAORFF010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27741_length_2629_cov_3.993007"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1480)
                     /locus_tag="PKK76_17300"
                     /pseudo
     CDS             complement(1..1480)
                     /locus_tag="PKK76_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
     gene            complement(1486..2031)
                     /locus_tag="PKK76_17305"
     CDS             complement(1486..2031)
                     /locus_tag="PKK76_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70290.1"
                     /translation="MPSTQDILIHAFHILLFLAASAIFVGAVVYRLKTVAKGQKAATL
                     ADMGARIRSFIFNVVLQQKLFKHPVRGAMHAFIFYGFLVYGLHTMSQMVAGNLWSFFK
                     SSGIEPYEFLIPDYIPAILTFKDPNQVLLCIAGLLITMTLTFLLYNNLKIGRSSKQIA
                     TLDQNGSLPFYSSHNSVLRLD"
     gene            2114..>2629
                     /locus_tag="PKK76_17310"
     CDS             2114..>2629
                     /locus_tag="PKK76_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNL70291.1"
                     /translation="MIHSPESILARAEQGERITPEEALTLYREGDFLAIQKTARELRN
                     RRHDPGVATYTMFRVVNYTNFCNVECTFCSFMDEIGSGKGYVLTTDQIRDKMKESLEI
                     GCDQMFLQGGVYPELPFEYYTDVLSATKSLGDIHIRGFSPVELISMEKTFGMSLREVV
                     RKLKAAGLDSVP"
BASE COUNT          641 a          627 c          696 g          665 t
ORIGIN      
        1 ggcggcgtcc tcgaggacgt agccgttgag gtccttgatg gtcggacgct tctggaatgc
       61 cacggccgag agtttgttct acaatgttgc gaggttgatc gtatacatcg ttgtaacgtc
      121 ccaggtagca agagtcatgg taagtaccgc gacgagactt catatcctct acagtcgcag
      181 gatccatttc tactttgcca tcgcgaatga gatcgtctat gaaatcagtg tgatgctgca
      241 cttcatagtt tccacccatt tgcgggtatt cgttcttcag tgtgttgtag caatgtggac
      301 acgcggttac aacttttttc acgttgtaac gattcatggt ttcgatgttt gtctgtgcga
      361 gagtctggaa gagatactcg tttccaccgc ggcgtgcgct atctcctgtg caattttctt
      421 ccgtgcccag gatgctgaac ttcacgcccg caccttgcat gatcttcacg aaagaacgtg
      481 cgatggtttt gttgcgatcg tcaaaagatc cagcgcatcc aacccagtag agtacatcta
      541 cgtttgaatc ttctgccatg gtcttcacgt tcagatcagc acaccagtcc gctcttgtat
      601 gagctcccac accccatggg ttggagttgt tttccatatt ctggaacgcg agctgaaggt
      661 gagaaggaaa acggctttct gctagaacca ggttgcggcg catttctaga atggcttcta
      721 gctggttgtt tcctacaggg caggcttcta cgcatgcgta gcaggaagta catccccaca
      781 gttcttcttc tgtgatgaaa ggatctccaa tgagtgctgc tgctggatct tctgttttga
      841 gaagagtgtt cgtgttttcg atcatggaat gcttgatatc caccatgatc ttctttggat
      901 cgagaggttt gcctgtgcga ttggcaggac actgcactgt gcatctgccg cattcaatgc
      961 aggaaagacc gtcgagcatg ctggtccagg gaagttcgcg aacattgcct gctccataga
     1021 ctgctgcgtt ttccatgtcc atgtgagtca tctggccgcg cggggttccg cgaatcatga
     1081 agaagttcat gggggcgaag atgagatgag aatgcttgga aagtggaacc actaccatga
     1141 aaccgtatac ggttacaatg tgagcccacc atgcgaaatt gcgcactgcg agagcttccg
     1201 ctccaccgct catgccaagt gcagtgagga gcttgagaac ggagcttgaa acccaggtat
     1261 gtgttccttt gccgctgatt acggattgcg ccgcgcctcc ggtgagagtg gacaccatca
     1321 gtgttccaat catcagaaga acaatggccg attgcgtaga tggaacatcg agacctttgg
     1381 aacgaagagc ccatcttcta taagcaaaga atacgagccc ggttagaacc agagcagaca
     1441 tattctgaat gagcatttcg aatacttccg ttccagcgga tgtgatcagt ccaaacgcaa
     1501 gaccgaattg tgcgaggagt agaatggcaa agaaccattc tgatccagcg ttgcgatttg
     1561 ctttgaactt cttccaatct tgagattgtt atagagaaga aaggtcaaag tcatcgtgat
     1621 cagaagaccg gcgatgcaca gaagcacctg gtttggatct ttgaaagtca gtatggctgg
     1681 aatgtaatcc ggaatcagaa attcgtaggg ttcaatgccg cttgatttga agaaactcca
     1741 gaggttcccg gccaccatct ggctcatggt gtggaggccg tataccagga agccgtagaa
     1801 gatgaatgcg tgcatggctc cgcgcactgg atgcttgaat agtttttgct gcagaaccac
     1861 attgaatatg aacgatcgga tgcgagcgcc catatccgcg agagtcgcag ccttctggcc
     1921 tttagccacc gtcttgagac ggtaaaccac tgcgcccaca aagatggcgc ttgcggccag
     1981 gaaaaggaga atgtgaaaag cgtgaatgag gatatcttga gtcgaaggca tagctaaaac
     2041 cagggatatc cattcaggaa tttcgtcgag aaaaaagctg ttcctctagc gcgctctgcg
     2101 gcttctgaac gttatgatcc attcgccgga aagtatacta gcccgggcgg agcagggcga
     2161 acgcatcacg cccgaagagg ctttgaccct gtatcgcgaa ggggacttcc ttgcgatcca
     2221 gaagacggcc agagagctgc gaaaccgtcg tcatgatcca ggagtggcta cctacaccat
     2281 gtttcgcgtg gtcaactaca ccaacttctg caacgtagaa tgcactttct gcagttttat
     2341 ggatgaaatc ggatccggta agggctacgt acttaccaca gatcaaatcc gcgataaaat
     2401 gaaagagtcc ctggagattg gctgcgacca gatgttcctc cagggtggtg tctatccaga
     2461 gcttcctttt gagtattaca cggacgttct atccgctaca aaatctctcg gggacattca
     2521 tatccgcggc ttctctccag ttgaattgat cagcatggaa aagacgttcg gcatgagtct
     2581 gcgcgaagtg gtgcgaaagc tcaaggctgc cggtcttgat tctgtgcca
//
LOCUS       DAORFF010000558         2628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_27752_length_2628_cov_3.723280, whole genome shotgun sequence.
ACCESSION   DAORFF010000558 DAORFF010000000
VERSION     DAORFF010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27752_length_2628_cov_3.723280"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..289
                     /locus_tag="PKK76_17315"
     CDS             26..289
                     /locus_tag="PKK76_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70292.1"
                     /translation="MRDKSGALRATEDLIFFPADHLPALARHFQVQTTPGQRSEGCEA
                     SVRAKIPVMRHFLITDGRSECGARRSAVRAVPAFEAARDAESP"
     gene            320..1363
                     /gene="dctP"
                     /locus_tag="PKK76_17320"
     CDS             320..1363
                     /gene="dctP"
                     /locus_tag="PKK76_17320"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNL70293.1"
                     /translation="MRIHTMSSYRPYAALCVFALAFFHAALNPLTAEVELRVATLAPS
                     GSNWMSEMEKSSMEIKDKTNGRVTVKYFPGGQQGDERDYVRKIKLGQLDGATVTSVGL
                     SMIDESIRVLELPDMFESAEELDYVADKIWPHFQAKFKAKGFRLGARGEVGWTYFLSK
                     NKVESLSQLRAQKAWVWGDDVIARTLFANLGMNGVPLGVPEVDAALTTGKVDMCYGSP
                     LAAVALQWYTKIKYITSMPLNFSVGATVYWEDSINKVTAEDQKTIEEIDRASSKKIRK
                     VARKSNEDAINTMKRRGVAVVETPAQMVTEFGNHAEQIRTTLTGKAFSKQDLDLVLRY
                     RSDHRIKRGGKPK"
     gene            complement(1426..>2628)
                     /locus_tag="PKK76_17325"
     CDS             complement(1426..>2628)
                     /locus_tag="PKK76_17325"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL70294.1"
                     /translation="QNTLQTVQALKAQQDADYYLTNLLANPLFKNYNRSKDVKTEFFV
                     KQKKEFTFRNKAGELGGDMCVTGNLKIRGKRYTMFVNGDAMGKSMQGAGGALIMGSVV
                     NSIMSRSASNKRDLDIDPKDWLRDTYNEIHNVFLTFDGSMYVSCIMGLIDDATGHVVF
                     FNAEHPMAVLYRKGRAVFIEEDIYLRKLGSQLQGSYQVTEANLEPGDVLFIGSDGRDD
                     INLTRDATNRQINEDETLFLRLIEQAEGNLDRIIELILKQGDITDDLSLIKIEFQPQG
                     QFSAAPEINLPELVDHMKNHRFEEALASLEKVDTNAEGALALYYRGLCLERLGRENEA
                     TPFLEKAIELDQQHVHALKLLGNVYFKLGKYRESALSLERAVELAPDQEKLKAALGKV
                     KQRMDRLN"
BASE COUNT          632 a          652 c          678 g          666 t
ORIGIN      
        1 cggttgcatg taatgcgtca tgtgagtgcg tgataaatcc ggggcactcc gggctacgga
       61 agacctgatc ttcttcccgg cagatcattt gcctgccctt gcgcggcatt ttcaagtcca
      121 gacaactccc ggccagcgat ctgaagggtg cgaagcatcc gttcgtgcga aaattcccgt
      181 tatgcgacat ttcctcatca cggacggacg aagcgaatgc ggagcccgga ggagcgcggt
      241 ccgagcagtc cctgcgttcg aggccgcacg agacgctgaa tctccttgac gtaaatatct
      301 ggtaaaggcg agtgaaggca tgcgcattca cacaatgtcc tcatatagac catacgccgc
      361 cctttgcgtg ttcgcactgg ccttctttca tgctgccttg aatcctttga ctgctgaggt
      421 ggaattgcgc gtcgcgaccc tggccccgag cggtagcaac tggatgtcgg aaatggaaaa
      481 atcttctatg gagatcaaag acaagactaa cggtagggtc acggtcaaat acttcccagg
      541 cggccaacag ggagatgagc gcgactacgt tcgcaagatc aagcttggcc agcttgatgg
      601 agcgactgtc acatcggtcg gactctctat gatagatgaa tcgatccgcg tgctcgaact
      661 gccggatatg ttcgagagtg ctgaagaact ggactacgtt gcggacaaaa tctggcctca
      721 ctttcaggca aagttcaaag cgaaaggatt caggctgggc gccaggggag aggtcggctg
      781 gacatacttt ctgtctaaga acaaagtgga atcactttcg cagcttcgcg cacaaaaggc
      841 ttgggtctgg ggtgacgacg tcattgctcg cacgttattc gccaatctgg gaatgaacgg
      901 tgtgcctctg ggagtaccgg aagtggatgc cgctctgacg accggtaagg ttgacatgtg
      961 ctatggatcg ccgcttgctg ccgtggctct gcaatggtat accaaaatca agtatataac
     1021 ttccatgcca ttgaacttct ccgtcggcgc gaccgtatac tgggaagact caatcaacaa
     1081 agtcactgcg gaagatcaaa agacaataga agaaatagat cgtgcctctt ctaaaaagat
     1141 tcgcaaagtc gcgagaaaaa gtaacgaaga cgccatcaac acgatgaaac gacgcggagt
     1201 cgcggtagtg gaaactcctg ctcaaatggt caccgagttt ggaaatcatg cagaacaaat
     1261 acgcacgacg ttgactggaa aggcgttctc aaaacaggat ctggatctgg ttttgcgtta
     1321 tcggagtgat catcgcataa agaggggcgg aaaaccaaag tagcgcggat tgcatgaagg
     1381 aatttcgcag cgcgaaattc cttctgctgg aaggcgcacg agacgtcaat tcaggcgatc
     1441 catcctctgc ttcacttttc cgagagccgc tttcagtttt tcctggtctg gcgccagttc
     1501 gactgctctt tccagagaga gagcggattc tctgtacttc ccaagtttga aatagacatt
     1561 ccccagtagc ttgagtgcat gcacatgctg ctggtcgagt tcaatcgctt tttcaagaaa
     1621 tggggtggct tcgttttcgc gtccgagtct ttccagacaa aggccacgat agtacagtgc
     1681 cagagcgcct tctgcgttcg tgtctacttt ctccagtgat gcgagagcct cttcgaatct
     1741 atgattcttc atgtgatcta caagctccgg aagattgatt tcaggcgccg cactgaactg
     1801 gccctgcggt tggaactcaa tcttgatcag agaaagatcg tccgtgatgt ctccttgctt
     1861 caaaatcaat tcaatgatgc gatccagatt tccttccgcc tgttcgatca agcgaaggaa
     1921 caaggtttca tcttcgttga tctgtctgtt tgtagcatcg cgtgttagat tgatatcatc
     1981 gcgtccgtct gaaccaatga acagaacatc tcctggttcc agattcgcct ctgtgacctg
     2041 gtaagatccc tgcaattgcg aaccaagctt tcgcagatag atgtcctctt ctataaaaac
     2101 cgcgcgtccc tttctataaa ggacggccat tggatgctcc gcgttgaaaa agacgacatg
     2161 tcctgtcgca tcgtcaatga gtcccatgat gcaggaaaca tacatggagc cgtcaaacgt
     2221 caaaaacaca ttgtgaattt cattgtaagt gtcccgcagc cagtcctttg gatcgatgtc
     2281 cagatctcgc ttgttggacg cagagcgaga catgatcgag ttgaccacgg agcccatgat
     2341 gagtgcacct ccggctcctt gcatcgattt tcccatcgca tctccgttga cgaacatcgt
     2401 ataacgtttg ccgcggatct ttagatttcc ggttacgcac atatccccgc caagctctcc
     2461 tgctttgttg cggaatgtga attctttctt ttgtttcacg aagaactcag tttttacgtc
     2521 cttgcttcga ttgtagttct taaagagtgg gtttgcgaga aggttggtca gatagtagtc
     2581 tgcatcctgt tgggctttga gcgcctgcac tgtttgcagt gtgttctg
//
LOCUS       DAORFF010000559         2615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_27979_length_2615_cov_3.248828, whole genome shotgun sequence.
ACCESSION   DAORFF010000559 DAORFF010000000
VERSION     DAORFF010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27979_length_2615_cov_3.248828"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PKK76_17330"
     CDS             <1..603
                     /locus_tag="PKK76_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70295.1"
                     /translation="QTLSVILPDGTAILVDSLDSSLHAFDLMRGSAEGKVQLGKSDVS
                     TTGLALGWASGDITTNLSGYSEGLTGMRGDLKIGLRDVNFVRFPPAAANLVPIRPDKI
                     KVSSLELPLSFRDGGLVLQNGLIASNLFIVKINTKGSYQGPSLDGMNLDGQICLKPDR
                     ELEQKEPDVFGMYVFAGGAAGGELCFQVRGTVGRPQFNKL"
     gene            600..1733
                     /locus_tag="PKK76_17335"
     CDS             600..1733
                     /locus_tag="PKK76_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70296.1"
                     /translation="MKTLYAIILLLALSSLSAREDMPFIPGPADLKKQTRPQTTPPNV
                     QPRDQKLQPQTGPGKPNDQTSIRDGKPAEPVPMKGDRPDLPSPGNTIRFEQAQTPRDF
                     PYQEYSRLMTDSIRLAAERSKMEPEIALHREQEIREELRLLFPRGLYRFGSTCPLKKL
                     FSSSAIEFRITCLPDDVQNKIRFARGRTDAIRNAFEFLSEGRTITANLQLLETGVGSD
                     GLEFIFREKSFQYENRFIEALRDESHMTEQDLLQPEWVSRKLPGIISQSYTESDLAAA
                     REVFPRSAWFPFHKDCPLKIQASTLLDGESTTRIELAPTCMPGRTAVVRVSEQEMNDE
                     SALRSDYGTALFAEMRLSGIMLVDGRLHLDWDGVRNVRRAEGK"
     gene            1730..>2615
                     /locus_tag="PKK76_17340"
     CDS             1730..>2615
                     /locus_tag="PKK76_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70297.1"
                     /translation="MKTWLVILFLFVQALSADTKSEKSDKQPREPAKTETAKTEPAKT
                     EPAKTEPAKAEPAKTDKPENKADRPERPSEKPPHAVCIEVTRRIPSAAMPYNGPSVKT
                     IRATGIQIGKRIVTDLENVRGAISLTVRQPDQDTATQGRLLFSGWDTGMAVIVSEEGD
                     EKAPLIIPDIPAITLDQEFGVSACSDRGLEMVRLRVDRIYESRLDDSDVGRFTLAGVS
                     ALGSIQPSASGAPVFSSGNLSGMFHSGKSNYVMPLSMIQRFLKDIDDGEYNGIPSLPF
                     EYQPLSHPDLRRYLSLPSS"
BASE COUNT          679 a          692 c          667 g          577 t
ORIGIN      
        1 cagacgttaa gcgtcattct tccagatgga acggccatac tcgtggatag cctcgactcc
       61 agccttcatg catttgatct aatgcgcggt tccgcggaag ggaaagtcca gttaggcaaa
      121 tcagatgttt ccacaacagg tcttgcactt ggatgggcca gcggagatat caccaccaat
      181 ctctccggct actcggaggg gctgaccggg atgcgcgggg atctgaagat tggattacgc
      241 gacgtcaact tcgtgcgatt cccaccggca gccgcgaacc ttgttcccat tcgcccggac
      301 aaaatcaaag tgagctctct ggagctgcca ctctcattca gggacggagg actggtgctt
      361 caaaacggcc tgatcgcaag caatttgttt attgtgaaaa tcaacacgaa aggctcctat
      421 caggggccat ctctagacgg tatgaacctg gacggccaga tttgtctgaa accggatcgg
      481 gagctggaac aaaaagaacc ggacgtattc gggatgtatg tgtttgctgg aggcgcggcc
      541 ggtggggaac tgtgcttcca ggtccgcgga acggtcggaa gaccgcaatt caacaaacta
      601 tgaagactct ctacgcaatc atcctgcttc ttgcactgtc ctccctttcc gccagagaag
      661 acatgccttt cattccggga cccgccgatc tgaaaaagca gacacgccct cagacaacgc
      721 cgcctaacgt tcaaccacgg gatcagaagc tgcaacctca aactggtcct ggcaagccca
      781 atgatcagac gtccatacga gatggtaaac cggcagaacc tgtacctatg aaaggcgatc
      841 gcccggatct accgtctccc ggaaacacaa ttcgctttga gcaggcacaa acacctcgtg
      901 acttcccata tcaggaatat tctcgcttga tgacagacag cattcgtctg gcagcagaac
      961 gcagcaaaat ggagccagaa attgcactcc atcgagaaca ggaaatccga gaagagttgc
     1021 gtcttctgtt tcccaggggt ctttatcgtt ttggttctac ttgtcctctc aagaagcttt
     1081 tttcatccag tgcgatcgaa tttagaatta catgtctgcc cgatgatgtg cagaacaaga
     1141 ttcgctttgc gcgcggaaga acagacgcta tcagaaacgc tttcgaattt ctttcggaag
     1201 gccgcaccat cacagccaat cttcagcttc tcgaaaccgg ggtgggctcg gacggtctgg
     1261 agtttatatt ccgcgaaaaa tcttttcaat acgaaaatcg cttcatcgaa gctctgcgcg
     1321 acgaatctca tatgacggag caggatctgc ttcaacccga atgggtctca agaaagttgc
     1381 ccggaatcat atctcaatcc tatactgaat ccgatctcgc agccgcgcgt gaagtgttcc
     1441 cgcgttcagc ctggtttcca tttcacaaag actgtcctct taagatccag gcatccactc
     1501 ttctcgatgg cgaatcgacg acgaggatcg aacttgcacc cacgtgtatg cctggacgca
     1561 ccgcggtcgt gcgagtcagc gaacaggaaa tgaacgatga atccgcgctt cgcagcgact
     1621 atggaacggc acttttcgcc gagatgaggc tttctggaat catgctggta gatggacgat
     1681 tgcatctgga ctgggacgga gttcgcaacg tcaggagagc agaaggcaaa tgaaaacctg
     1741 gttggtaatt ctattccttt ttgtacaggc tctgagcgcg gatacaaaat cagagaagtc
     1801 tgataagcaa ccgcgcgagc ccgcgaaaac ggaaaccgca aaaacagaac ccgctaaaac
     1861 cgagcctgca aagacagaac ctgcaaaagc ggaacctgcg aaaacggata agccagagaa
     1921 taaagcagat aggccggaaa gaccgtcgga gaaacctcct cacgcagttt gcatagaagt
     1981 cacacgcaga ataccatcag ccgcaatgcc gtataacggt ccttccgtga aaacgattcg
     2041 cgccaccgga attcagatcg gaaaaaggat tgtaacagat ctagagaatg tgcgaggagc
     2101 catttctctc acagtccgcc agccggacca ggatacggcg acacagggaa ggctgctctt
     2161 cagtggctgg gatacaggaa tggcggtgat tgtctcagaa gaaggtgacg aaaaagcacc
     2221 attgatcatt cccgacatcc cggccatcac ccttgaccag gagttcgggg tgagcgcatg
     2281 ttcggatcgt gggctggaaa tggtccgtct tcgcgtggat cgcatctatg aatctcgtct
     2341 ggacgattcg gacgtgggac gcttcaccct ggccggagtc agtgccctgg ggagcattca
     2401 accttccgcc agtggcgccc ccgtattttc ttccgggaat ctcagtggaa tgtttcattc
     2461 tggaaaatcg aactacgtca tgcctctgtc tatgattcag aggttcttga aagacattga
     2521 tgacggagaa tacaatggga ttccttcact tccttttgaa taccagccac tgagtcaccc
     2581 cgatctccgg cgctatttga gtttgccttc atctt
//
LOCUS       DAORFF010000560         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_28038_length_2612_cov_3.432147, whole genome shotgun sequence.
ACCESSION   DAORFF010000560 DAORFF010000000
VERSION     DAORFF010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28038_length_2612_cov_3.432147"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..776
                     /locus_tag="PKK76_17345"
     CDS             21..776
                     /locus_tag="PKK76_17345"
                     /inference="COORDINATES: protein motif:HMM:NF015921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70298.1"
                     /translation="MKDNANTLSKVPAVTLVFWILKILATTLGETAGDAVSMSLDLGY
                     FIGTAIFAVTFLVAAAVQIRTKQFHPVIYWITIIATTTVGTTMADLADRSLGIGYAGG
                     SSLLLFLLCTSLFIWHRVMGSISVDSINNWKSELFYWVTIMFSQTLGTALGDWTADSA
                     GFGYLGSAGIFGGALAVIALLYYATKISHTLLFWAAFILTRPLGAVVGDLLDKPLNEG
                     GLDLSRFAASAVLIALMACGIFFFKHRAAEETH"
     gene            complement(784..1542)
                     /locus_tag="PKK76_17350"
     CDS             complement(784..1542)
                     /locus_tag="PKK76_17350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL70299.1"
                     /translation="MGPLFLMALIVIIDSDLTQARALEGALAGLRHRILATQSAARGI
                     ELAQSGNPDLILLNPENSQMDGIQILASLRKDPITKEIAVALVQRQPDAALLPRLRQL
                     GVVDTISPGLTQDLLRKKILQSLVLAEKHKLDHNLKRANHIHVRRTPGRTDISILSGL
                     KEFSFQEAKTVLNPFFVRLTKNDSMVLDIRAIPNINETELPLLQQIIDLVANEKLILI
                     AGRHLGLLIAETEIGETVPTFFTQEEAEQHLSAR"
     gene            complement(1579..2520)
                     /gene="msrB"
                     /locus_tag="PKK76_17355"
     CDS             complement(1579..2520)
                     /gene="msrB"
                     /locus_tag="PKK76_17355"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNL70300.1"
                     /translation="MEPPFEKLQGVSSVVSGYAGGQKANPTYEEVSSGGTGHAETVEV
                     TYDPGVVSYEKLLEVFFHNVDPTDAGGQFVDRGSQYRTVIFYYSEEQKAKAIQAKADL
                     AKSGKFRKAIVTEIVPIKVFYPAEEYHQDYYKKNPYSYKRYRSGSGRDEYLERVWGKQ
                     STSKEKRMYTKPPEEELKKKLTPLQYEVTQHEGTERPFQNEYWDNHKDGIYVDRVSGE
                     PLFSSKDKYDSRTGWPSFTKPLDPANIVTKKDMSVGMVRVEVRSKHGDSHLGHVFDDG
                     PAPTGQRYCMNSASLRFIEKSDLEKEGYEEFAKLFDK"
BASE COUNT          596 a          682 c          647 g          687 t
ORIGIN      
        1 caaactaaag gaactctcaa atgaaagaca atgcaaacac tctaagcaag gtaccggccg
       61 tgactcttgt tttctggatt ctaaaaatcc ttgccaccac tctcggagaa acggcaggag
      121 atgcggtgtc tatgtccctg gatctaggat acttcatagg aactgcgatc ttcgccgtta
      181 cctttcttgt cgcggcagcc gtgcaaatcc gaaccaaaca gtttcatccg gtgatctact
      241 ggatcacaat catcgctact acaaccgttg gcacgaccat ggcggatctc gcagatagat
      301 cccttggaat cgggtatgca ggcggatcgt ctcttttgct cttcttgctg tgcacttctc
      361 tcttcatctg gcacagagtg atgggttcga tttctgtgga ttccatcaac aactggaaat
      421 cagaattgtt ctactgggtc accattatgt tctcgcagac cctcggcacg gccctgggag
      481 actggacggc ggactccgca ggcttcggat atctgggaag tgcgggcatc ttcgggggag
      541 cgctcgcggt catcgcactg ctctactatg ccacgaagat ttcgcacact ctcttgttct
      601 gggcagcatt cattctgaca agacctctgg gtgcagtcgt cggggacctt ctggacaagc
      661 cactaaacga aggtggtctg gatctgagtc gatttgccgc ttccgctgtg ctgatcgctc
      721 ttatggcctg cggaatcttc tttttcaaac atcgcgcggc agaagaaacg cactgaatgc
      781 cgcctaacgg gccgacaaat gttgctccgc ttcttcctgt gtgaagaaag tgggcacagt
      841 ttctccaatc tcggtttcag caatcagcaa accgagatgg cggcctgcga tgagtataag
      901 cttttcgttt gccaccaggt cgatgatctg ctgcaaaagg ggcaattctg tttcgttgat
      961 gtttggaatg gcacgaatgt ccaggaccat ggagtcattc tttgtaagcc tcacaaagaa
     1021 tggattgaga actgtcttcg cttcttgaaa cgaaaactct ttgaggcccg aaaggataga
     1081 gatgtctgtc ctgccgggag tgcgacgaac gtggatgtga ttggctcgct tcaggttgtg
     1141 atcgagttta tgcttttctg cgagaacgag agactgcagg atcttctttc gcaaaagatc
     1201 ctgcgttaga cctggcgaaa ttgtatctac gacacccagc tgtcgcagac gtggaaggag
     1261 agctgcatcc ggttgacgct gcaccagagc gactgcgatt tcttttgtga ttggatcctt
     1321 gcgaagcgag gccagaatct ggatgccatc catctgcgag ttttctggat tgaggaggat
     1381 gagatccggg tttccacttt gcgccagttc aatcccgcgc gctgcggatt gagtggccag
     1441 aatgcgatgg cgaagaccgg ccagcgcacc ctcaagcgct ctggcctgcg tcaaatcaga
     1501 gtcaatgatg acgatcaggg ccatgaggaa caaaggcccc atattgcccc aatggtcaag
     1561 tcgactctga tccaagattc atttatcgaa gagttttgcg aattcctcgt agccctcttt
     1621 ctccaaatca ctcttctcaa tgaagcgaag ggatgcagag ttcatgcagt accgctggcc
     1681 tgtaggagcg gggccgtcat caaacacatg acccagatgc gagtcaccat gcttgcttct
     1741 cacttctact cgcaccattc ccacagacat atccttcttt gtgactatat tggcgggatc
     1801 cagaggctta gtgaagctgg gccagccagt acgcgaatcg tacttatcct ttgaactgaa
     1861 aagaggctct cccgaaacgc gatcgacgta gattccgtct ttatgattgt cccagtattc
     1921 attctgaaat gggcgctctg tgccctcgtg ctgcgtcacc tcatattgaa gaggcgtcag
     1981 tttcttcttg agttcttctt ctggtggctt cgtgtacatt cttttctcct tgctggtgga
     2041 ttgcttgccc catactctct ccagatactc atcgcgccct gaaccggagc gatacctctt
     2101 gtagctgtac gggttcttct tgtaataatc ttgatgatat tcttccgccg ggtaaaacac
     2161 tttgatcggc acaatttctg taacgatggc cttgcgaaac ttacccgact tcgccagatc
     2221 tgcctttgcc tgaatggcct ttgccttctg ctcttctgag taatagaaaa taacggttct
     2281 gtactgcgaa cccctgtcta caaactggcc gccggcatcc gttggatcca cgttgtgaaa
     2341 aaaaacttcc aggagttttt cgtacgatac aacacctgga tcatacgtca cttccacagt
     2401 ttccgcgtga ccggttccac cgcttgaaac ctcctcataa gtgggattcg ctttttgtcc
     2461 accggcataa ccggagacaa cactggaaac tccttgtaac ttctcaaaag gtggttccat
     2521 gcaccagaaa caaccgcctg caaaggccgc ctttgcagtg cgtggcccgg cgatgatgga
     2581 acaggatgag agtaacagac aaagaacgct ta
//
LOCUS       DAORFF010000561         2609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_28103_length_2609_cov_3.223179, whole genome shotgun sequence.
ACCESSION   DAORFF010000561 DAORFF010000000
VERSION     DAORFF010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28103_length_2609_cov_3.223179"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PKK76_17360"
     CDS             <1..674
                     /locus_tag="PKK76_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNL70301.1"
                     /translation="KEEDVAKAWQYAMTGGRAKGGRVIVEEWIPFDYEITLLTVRHSG
                     GTDFMNPIGHLQIDGDYRESWTPQPMSEKALTAAQSIAKSITDGLGGRGIFGVELFVK
                     GDQVYFSEVSPRPHDTGLVTLISQNRSEFALHARAILGLPIGTIHSYGPAASCALLME
                     GDSDAPVLEGLEDALNGADTDFRLFGKPEIHGKRRLGVALALGQTVEKAREAARNSAA
                     SIRLL"
     gene            complement(669..890)
                     /locus_tag="PKK76_17365"
     CDS             complement(669..890)
                     /locus_tag="PKK76_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70302.1"
                     /translation="MLKIGNGLGILPDDVLELLYIWPIRVFQDQAEWSLYAENLAFAS
                     RHDSDRHIPSPGLHRLTSDSGPGVHKTKS"
     gene            936..1127
                     /locus_tag="PKK76_17370"
     CDS             936..1127
                     /locus_tag="PKK76_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70303.1"
                     /translation="MAEVGDDRHKRRAEALRNHAMEELQIAGRYENDPDRLRACMMRL
                     SSTLIDLKQVANEEEVQGH"
     gene            1179..1532
                     /locus_tag="PKK76_17375"
     CDS             1179..1532
                     /locus_tag="PKK76_17375"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNL70304.1"
                     /translation="MAKSASKFSFLDVLQEGILEIAEAGKNGTVKLKRSDLKALIEKA
                     FQQGVKAAAGGERVKFPVVGTLLRKDVEAKKGGKGRNPFTGEEIMIKPRPATKKPRWS
                     FPKSIKEDFANKKNW"
     gene            1543..2403
                     /locus_tag="PKK76_17380"
     CDS             1543..2403
                     /locus_tag="PKK76_17380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNL70305.1"
                     /translation="MESFLELQGVRVGFPIQKGIFRRTHGHKIVLDSVSFKIARGKTV
                     GLVGESGSGKTTIGRAILGLVPVMSGSIPLHLDDPLISREPLDLAHGFPDDFDPSTGQ
                     VRSLSRMLRRRLQIIFQDPYSSLNPRLRIGDVLLEAMHLHFPEMTEALRRKKAKDTLD
                     RVGLSDSALERYPHEFSGGQRQRISIARALVLNPEFIVCDECVSALDVSIQAQILNLL
                     RDLQEERRLTYLFISHDLSVVRYISHEICVLDRGTIVEQGDAARVVDHPQQPYTKRLL
                     EAVPSLIPRR"
BASE COUNT          625 a          645 c          732 g          607 t
ORIGIN      
        1 gaaaagagga ggatgtagcg aaggcctggc agtatgcgat gacaggaggg cgtgcgaaag
       61 gcggacgcgt cattgttgag gaatggatcc cctttgatta cgaaatcact ctactgaccg
      121 tgcgccattc aggcgggacg gatttcatga acccgatcgg tcacctacag atagatggcg
      181 actatcgtga atcgtggacg ccgcaaccaa tgagcgagaa ggctctgact gcggcgcagt
      241 cgattgcgaa gtccatcacg gacggtctgg gagggcgggg catctttggc gtggagcttt
      301 ttgtaaaagg ggatcaggtc tatttcagcg aagtttctcc cagaccgcat gacacgggtc
      361 ttgtaacttt gatttcgcag aatcgttccg agttcgcact gcatgcccgc gccatccttg
      421 gcctgccgat cggcacaatt cattcgtatg gtccggccgc atcttgcgcc ttgcttatgg
      481 aaggagattc ggatgccccg gttctggaag gcctggagga tgctttgaac ggagcagata
      541 cggattttag actttttgga aagcctgaga tccacggaaa gaggcgcctg ggtgtggcgc
      601 ttgccctggg ccagactgta gagaaggcgc gtgaggcggc gagaaattcg gccgcaagca
      661 ttcgactcct atgattttgt cttgtgaacg cccgggccag agtcagaagt gagtcgatga
      721 agtcccgggg acggaatatg acgatcggaa tcgtgcctgc tcgcgaaagc gaggttttcc
      781 gcatacaggg accattccgc ctggtcctgg aagacacgga tcggccaaat gtaaagaagc
      841 tcaagcacgt catccgggag aatccccaga ccgtttccga ttttaagcat ttcatccatt
      901 atctaacggg tcagatcagg cgggccgcgg accttttggc tgaagttggg gatgatcgcc
      961 acaaaagacg agcagaagca ctccgcaatc atgcgatgga ggaactgcag atagccgggc
     1021 gttacgagaa tgatccagat cgccttcgcg catgcatgat gagattgagt tctactttga
     1081 tcgatctaaa gcaggtagcg aacgaagaag aagtgcaggg tcattgattt tgaaatttta
     1141 cttgcaggat atcttccaat aaaaagaaac aacaggatat ggccaaatca gctagcaaat
     1201 tctcttttct tgatgttctc caggaaggca ttcttgaaat cgcagaagcc ggaaaaaacg
     1261 gcacagtgaa attgaagcgc agtgatctca aagcgctgat cgaaaaagca ttccagcagg
     1321 gagtcaaagc agccgccggc ggcgagcgtg tgaagttccc ggtagtcggt actctccttc
     1381 gcaaggacgt ggaagcaaag aaaggcggca agggccgtaa tcctttcacg ggcgaagaga
     1441 tcatgatcaa gccgcgccct gcaaccaaga aaccaagatg gtctttcccg aagtctatca
     1501 aagaagactt cgcaaacaag aagaactggt aatccaaaag ccatggagtc cttcctcgaa
     1561 ttgcaaggcg ttcgagtcgg gttcccaatc cagaaaggta tctttcgaag gactcatggc
     1621 cataagatcg tactcgactc tgtttcgttc aaaatagccc gcggcaagac cgtcggtctg
     1681 gtgggtgaat caggttccgg caagactacc atcggacgtg caatcctcgg gcttgtgcct
     1741 gtgatgtccg gttccattcc gcttcatctg gatgatcctc tcatctccag ggaaccactg
     1801 gatctcgccc acggttttcc agacgacttt gatccatcta ccggacaggt tcgttctctt
     1861 tcgcgcatgc ttcggcgcag attgcagatt atttttcaag atccttattc cagtttgaat
     1921 ccgcgccttc gcatcggaga cgtactgcta gaagcaatgc atttgcattt tccagaaatg
     1981 acagaagcgt tgcggagaaa aaaggcgaaa gacactctgg acagagtggg actttctgat
     2041 tcggctctgg agcgctaccc tcatgaattc agcggaggcc agagacagag aatcagcatc
     2101 gcgcgcgctc tggttctgaa tcctgagttc attgtgtgcg acgaatgtgt cagcgcgctg
     2161 gacgtgtcta ttcaggcgca gatcttaaac ctgcttcgtg atctacagga agaacgccgt
     2221 ctaacgtatt tgttcatctc tcacgattta tctgtagtac gttacatttc acacgaaatc
     2281 tgtgttctgg atcgcgggac aattgtagag cagggtgatg cggcccgcgt ggtggatcat
     2341 ccacagcagc cttatacaaa gcggctgctg gaagcggtgc cgtcactcat tccgagacgt
     2401 tgatttcgat atctactccc gaccgggtcc cgcccaaacc cggcgaaacg ggcgtccttg
     2461 cccgagttcg ccgggcgctc cgacttcctg tctccgcaat ttggggggaa cccggtggga
     2521 gtgactctca accaagtctc ggatgcacgg gtggcctgag ccgtaacttc atttgcctcg
     2581 cttctttttt tgggtttggg ggtttgttt
//
LOCUS       DAORFF010000562         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_28118_length_2608_cov_3.881316, whole genome shotgun sequence.
ACCESSION   DAORFF010000562 DAORFF010000000
VERSION     DAORFF010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28118_length_2608_cov_3.881316"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKK76_17385"
     CDS             complement(<1..299)
                     /locus_tag="PKK76_17385"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNL70306.1"
                     /translation="MRYSADAVLIFCTLLWGLTFSLVKESLSETGPLYINVLRFVFSG
                     VLFLPAAILRRRQVRLSTVTWGFGLGVLLFYGFAFQTMGLGTTTAQRSAFLTQMLV"
     gene            444..1730
                     /locus_tag="PKK76_17390"
     CDS             444..1730
                     /locus_tag="PKK76_17390"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70307.1"
                     /translation="MLQVGAIILSFEEAGDLTALTPWKAVDVWSLRDVLGDLVLGFVP
                     VSTCNRVEFYYTADTDCHSQILDRVISFLPPLANGITPSHQTGRSAARHLIRLASGLE
                     SMVLGETEIRAQLKQAYEDAVKHNTLDRRLRILFQHVFQESRGIRQEVPMSRLPLSVA
                     TIATRHMLRLIAPQTEYLKEASLPHTAVVIGSGPMSRQSAESLSRHFQSIVLVNRTVS
                     KIEPLAARLGASVLSFEEFVAAPERVCEGGKFPATIVTATSRDDAFLTPELVNRILKQ
                     RGGSEKLCIVDMALPADVHPDCATDERVSIITMETIRKELDGNREKRAEAARMAADAI
                     EKALIRTEAAMISGVSSTLLKDIQKEVRDKSRQELDALLGNRLSHLSAKDIRMLYDWA
                     IRAHKEMNRIHRKGLESVLEHYYGSENGAPLAERAE"
     gene            1727..>2608
                     /gene="hemC"
                     /locus_tag="PKK76_17395"
     CDS             1727..>2608
                     /gene="hemC"
                     /locus_tag="PKK76_17395"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNL70308.1"
                     /translation="MNPRLRIATRGSHLALAQSRYIQSRIAATPNAPQTELVIIKTSG
                     DANQVLSLAEAASGDARKGLFTKEIEEALLGDQADIAVHSFKDLPTQMVDGLRICAVP
                     QRIESLDILVFPKVKKAYDRLPYVREGSTIGTSSARRVAQLKYLFPEIKPVLLRGNVP
                     TRMRRLLAPDGPDATLLSAAGIERLSREGFFDGKDPDSVDLSLFEIIPIPASIMVPAP
                     AQGTLAIQCRITDSRAIAATSHLHDEELARIIAGEREILARLEGGCHLPLGTHMTREK
                     QKGKTYFQMHMFLGEEAI"
BASE COUNT          685 a          721 c          656 g          546 t
ORIGIN      
        1 acaagcattt gagtcagaaa cgcggacctt tgtgcagtag tggttccaag gcccatcgtc
       61 tggaaggcga acccgtaaaa caaaagcaca cccaggccaa atccccacgt aacagtggac
      121 agacgaacct gacgccgtcg aagaatggcc gcgggcaaaa ataaaacccc ggaaaaaaca
      181 aatcgcagca cattgatgta aagagggcct gtttcggaga gagattcctt caccaggcta
      241 aacgtaaggc cccacagcag cgtacagaag atgagcactg cgtccgcact gtagcgcatg
      301 ggtcaaattc tgctaagaag gaccctctgc aagcgttctg gcttcttgat actgcgtctc
      361 aagtccgact ggtttgcccg tcccctgaca actccatgac aaaagaagta ctctgaggcc
      421 ataaaatatg aaactgaaca gccatgcttc aggtcggggc aatcattctc agttttgaag
      481 aagccgggga tctcactgcg cttacgccgt ggaaggctgt ggacgtctgg tctctgcgcg
      541 atgtcctggg cgatctggtt ctgggctttg ttccagtgtc cacatgcaac cgtgttgaat
      601 tttactatac ggcagatacg gactgtcact cgcagattct ggatcgcgtc atttctttcc
      661 ttcctcctct cgcaaacgga atcacccctt ctcaccaaac aggccgcagt gcggccagac
      721 atctcatccg attggccagt ggcctggagt ccatggttct cggggaaaca gagattcgag
      781 cgcaactgaa acaggcatac gaagacgcag tcaaacacaa cactctggac cgcaggcttc
      841 gcattctctt tcagcatgtt ttccaggagt cgcgcggcat ccggcaagaa gttcccatga
      901 gtcgccttcc gttgtctgta gccacgattg caacgcgcca tatgctgcgg ctgatcgcac
      961 ctcagaccga gtatttaaag gaagcgagtc tgccacacac tgcggtcgtc attggttctg
     1021 gtcccatgag tcgccaatcc gcagagtcgc tctcgcgaca ctttcaatcc atcgttctag
     1081 taaaccgcac tgtttccaaa atagaaccac tggctgcgag actgggtgct tctgttctat
     1141 catttgaaga gttcgtggcg gcgccggaaa gagtctgcga aggcggaaaa ttccctgcaa
     1201 ccatcgtaac agccacatcg agagatgatg cattcctcac acctgaactt gtaaatagaa
     1261 tcctcaaaca acgcggcgga agcgaaaagc tctgcatcgt agacatggca ttgccagctg
     1321 acgttcatcc ggattgcgcg accgacgagc gagtgtccat catcaccatg gaaaccatcc
     1381 gcaaagaact cgacggcaat cgcgaaaaac gcgcggaagc agcacgcatg gctgcagacg
     1441 cgatcgagaa agcactcatt cgcacggaag ctgccatgat ctctggagtc tcctccactc
     1501 tgctcaagga cattcaaaaa gaagttcgcg acaaatccag acaggaactg gatgcccttc
     1561 ttgggaatcg tctctctcac ctgagcgcga aagacattcg catgctctat gactgggcca
     1621 tacgtgcgca taaagaaatg aatcggatcc acagaaaggg tctcgaaagc gttctcgagc
     1681 actactacgg aagtgaaaat ggagcgcctc ttgcggagcg cgcggagtga atccgcggct
     1741 gcgaattgca accagaggat cccatctcgc tctggcacag agccgctaca tccagagcag
     1801 aatcgccgcc actcccaacg cgccacaaac tgaactcgtc atcatcaaaa cgtccgggga
     1861 tgcaaaccag gttctgtccc tcgcagaagc agcgagtggt gatgcgcgaa aaggcctgtt
     1921 tacaaaggag atcgaagaag ctctgttagg cgatcaggca gacattgcag ttcactcttt
     1981 caaagatttg cccacgcaaa tggtggatgg gcttagaatt tgcgccgtgc cgcagagaat
     2041 cgaatctctg gacatcctgg tgtttccaaa agtaaaaaaa gcctacgatc ggcttcccta
     2101 cgtgagagag ggatctacca ttggcacatc ttctgcccgc agagtggcgc aactcaagta
     2161 tctattccct gaaatcaagc cggtccttct tcgcggaaat gtgcctacac gcatgcgaag
     2221 actcctggcg ccagatggtc ctgacgcaac tctcctttcc gccgccggaa ttgaaagact
     2281 gagccgtgaa ggatttttcg acggcaaaga tcctgactct gtggatcttt ctctctttga
     2341 aatcattccc attcccgcat ccatcatggt tccagcgccc gcacagggaa cgctcgccat
     2401 tcaatgccgc ataacggata gtagagccat tgcggccaca tcgcacctgc acgatgaaga
     2461 actggcccgg atcattgcag gagaaagaga aattctagca cgtctcgagg gaggatgtca
     2521 tctaccgctc ggaactcata tgacacgaga aaagcagaag ggcaagacgt attttcaaat
     2581 gcatatgttt ctgggggaag aggccatc
//
LOCUS       DAORFF010000563         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_28331_length_2597_cov_3.277341, whole genome shotgun sequence.
ACCESSION   DAORFF010000563 DAORFF010000000
VERSION     DAORFF010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28331_length_2597_cov_3.277341"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="PKK76_17400"
     CDS             <1..161
                     /locus_tag="PKK76_17400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNL70309.1"
                     /translation="EGSPHTVVTLSIGGACEIPTPSSSWTSLISRADEALYRAKSEGR
                     NRYAYHKT"
     gene            complement(149..556)
                     /locus_tag="PKK76_17405"
     CDS             complement(149..556)
                     /locus_tag="PKK76_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70310.1"
                     /translation="MAKNFFWFVFALFVGTAIYTGWHPGFFDFNVRLGGLKLLVMLCF
                     AAFLGYSIYCSSRESLPATIQKMARLHWGRQIGIDLYIGLSIFLGIVFAMEGSPLAAA
                     AWILPTLLFGNLATLLYLSIHFDAIVSRLNQVL"
     gene            589..1254
                     /locus_tag="PKK76_17410"
     CDS             589..1254
                     /locus_tag="PKK76_17410"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNL70311.1"
                     /translation="MKPVKHLIYRKCSWIKFYCARKGLFQDSTMDNLRKDFLYPPGGI
                     LIWIIISLEVVTFAAGLIAFLVTGQSEKELFALSRPLLDRNIGWINTIVLLTGGLFMA
                     NAIHSIREGKRKSSSLWMIAAGLAGITFLVLKCVEYSSKLKGGHDLGSDSFFTLYWLL
                     TGFHFLHVALATVILLVLASKVQKGKYSKKSHLDLETAGSFWHMCDLIWLFIFPVVYL
                     LPG"
     gene            1259..1516
                     /locus_tag="PKK76_17415"
     CDS             1259..1516
                     /locus_tag="PKK76_17415"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNL70312.1"
                     /translation="MKRNTIVWLILIALTAASVTLSHAEFRRSYLIAGILGFALVKFL
                     GVAFQFMEIRTAHRGWKILLILFGVSFTVLAYAVSRGGVSL"
     gene            1513..2265
                     /locus_tag="PKK76_17420"
     CDS             1513..2265
                     /locus_tag="PKK76_17420"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNL70313.1"
                     /translation="MKIRLCQIQVDASAPEKNFSKIRQILTSASEDLIVFPEACTTGF
                     PYRRLTSITEANQTFLDEIALQSANRTGVILLPLLIEENNAIYNRQHWITGGKIAATY
                     DKIHLIGILGEDRFLTRGSAIVTVPWKPHIAGLATCYDLRFPELFRKLILKGADILTI
                     PAMWPIERAAHLHPLAKARAIENQAYVLVCNGCGPCGSMDLCGSSIAIDPKGEVIAQA
                     GSEEEQLTFEIDPAFAAEWRKDFPALGDIRLI"
BASE COUNT          675 a          674 c          616 g          632 t
ORIGIN      
        1 atgaagggag cccgcacaca gtggtcacgc tgagcattgg tggtgcgtgc gaaattccaa
       61 cgccttcttc cagctggacg tctctgattt cgcgcgccga cgaagccctg tatcgcgcaa
      121 aatcagaagg tcgcaatcgc tatgcttatc acaaaacctg attcaatcgc gaaacgatcg
      181 cgtcaaaatg aatcgagaga tacaggagag tagccagatt gccaaagagc aaagtgggta
      241 gaatccacgc cgcagccgcc agaggtgaac cttccatggc aaatacgatc cccaaaaaaa
      301 tggaaagccc aatatagaga tcaattccaa tctgtctgcc ccagtgcaaa cgagccatct
      361 tctgaatcgt ggccggaaga ctttctcgac tgctgcaata aatcgaataa ccaaggaagg
      421 cagcgaaaca caacattacc aggagtttca aaccgccgag tctgacattg aaatcaaaga
      481 acccaggatg ccatccggtg tagatggccg tcccaacaaa caacgcgaag acgaaccaga
      541 agaaattttt tgccatactt ggagcctctc ccttttttga ggcggcccgt gaaaccagtc
      601 aagcacttga tctatcgcaa gtgctcatgg atcaaattct actgtgcgcg aaagggcctc
      661 tttcaagatt caaccatgga caatctcaga aaagatttcc tctatccgcc gggtggcatc
      721 ctcatatgga tcatcatttc cctggaagtg gtcacgtttg ccgctggatt gattgcgttt
      781 ttagtaaccg gtcaatcgga gaaggaactg ttcgcacttt cgcgaccact gctggatcgc
      841 aacattggat ggatcaacac gatcgttctc ttgaccggag gactcttcat ggcaaacgcc
      901 atccactcaa tcagagaagg caaacgcaaa tcgagcagtc tctggatgat agcagcaggt
      961 cttgcgggaa tcacatttct ggttctcaag tgcgtagaat actcttccaa gttgaaggga
     1021 ggtcacgacc tggggtcgga ctcttttttc actctctact ggctactgac cggttttcac
     1081 tttctccatg tggctctggc cactgtcata cttctagtgc tggccagcaa agttcaaaaa
     1141 ggtaaatatt ctaaaaaatc acatctggat ctggaaacgg caggttcgtt ctggcatatg
     1201 tgcgatctca tctggttgtt catctttccg gtcgtctact tgctcccggg ataatggcat
     1261 gaaaagaaac acaattgtct ggttgattct aatcgcactg acggctgcca gcgtcacgct
     1321 ttcgcatgcg gaatttcgta ggtcctattt gattgcgggt attctgggct tcgctcttgt
     1381 aaaatttctg ggagtcgctt tccaattcat ggaaattcgc accgctcatc gcggctggaa
     1441 aatcctgttg attctgtttg gcgtatcctt cactgtgctc gcatacgctg tctcccgagg
     1501 gggcgtctcc ctatgaaaat tcgcctctgt cagatacaag tcgacgcatc cgcaccggag
     1561 aagaattttt caaagatcag gcaaatcctt acatccgcaa gcgaagacct gatcgtattt
     1621 ccggaggcct gcacgacggg ctttccttat cgccgcctaa cgtccatcac ggaggcaaac
     1681 cagacctttc tagatgaaat cgcacttcaa agcgcgaacc gcaccggggt tattctgctg
     1741 cctcttctta tagaagaaaa caacgccatt tacaatcgtc agcattggat cacaggaggc
     1801 aagatcgcag ccacgtatga caaaatccat ttgatcggga ttctggggga agatcgattc
     1861 ctcactcgtg gctcagccat cgtaactgtg ccctggaaac cccacattgc aggacttgcc
     1921 acttgctacg accttcgctt tcccgaactc tttcgcaaac tcatcctgaa aggggcggac
     1981 atcctgacca ttccagctat gtggcccatc gaacgagctg ctcatttgca tccactggcg
     2041 aaggcacgtg ccatcgaaaa ccaggcatat gtactcgttt gcaacggatg cgggccgtgc
     2101 ggctccatgg atctatgcgg aagctccatt gcaattgatc caaaagggga agtgattgcg
     2161 caagcgggga gcgaagaaga gcaactaacg ttcgaaatcg acccggcttt cgcggctgaa
     2221 tggagaaaag attttccagc cctcggcgac attcgtttga tttgaagaaa cagcctggcg
     2281 caacgtcagg ttgagtgcca aacgattgtc gcgaccaaac actgtccata gacaacgcac
     2341 gcccaaatca acttttttcc cggaacaggc ctttattctg gacgcgccag atgagcagac
     2401 ccggcaagac aaaatacgta gaaatttaca cggtttacaa ttgacgaaat atagatcgta
     2461 tgattatttg gcagggtgca gcggaggttc acatgaagca atcgttcgga tccgccctca
     2521 tccatatttg cctgtggtta ttgtttcttt tgtccatcgt attcttcgca tggaatcttt
     2581 cacatgggcc ggcgaat
//
LOCUS       DAORFF010000564         2593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_28403_length_2593_cov_3.127266, whole genome shotgun sequence.
ACCESSION   DAORFF010000564 DAORFF010000000
VERSION     DAORFF010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28403_length_2593_cov_3.127266"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PKK76_17425"
     CDS             <1..693
                     /locus_tag="PKK76_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNL70314.1"
                     /translation="KLLKLPFVKEIIVVDDCSKDRTAEIVEEKRRRNRKIRLLRHSEN
                     QGKTAAIKTAVQDAQGSIVIIQDADLEYSPSEMINVVSPIINGVADVVYGSRFLVRRE
                     ARVLYYGHFLANRFLTTLSNIFTNLNMTDIETGYKALRTPLLKSLVLTSSGFGMEAEI
                     TAHIARLPIRIYETPISYYGRTYEEGKKIGMKDGIMAILYIFYYNILGVRLPSARKYR
                     NTVRDFLSEIKR"
     gene            690..1070
                     /locus_tag="PKK76_17430"
     CDS             690..1070
                     /locus_tag="PKK76_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70315.1"
                     /translation="MTSGRGVSVYLSLCLAFLLGLTWTAAGTIRADKHTRILQTAINE
                     MRSHRGIKAERVELCRSAHQIATEFSVAATYAMPGLSFVEVEKCAGPGHSIRFFLTDD
                     SQVSPAMEHQILFKGKGLVIVEAL"
     gene            1067..2266
                     /locus_tag="PKK76_17435"
     CDS             1067..2266
                     /locus_tag="PKK76_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70316.1"
                     /translation="MIQSSLRLSLFNLGLWFCLSYALLALVPPGSVNVFRVLFAAIFL
                     IANFFVARRLSWKEPGALRERSWLVFLLCFLSALILFFLRMSAHPHGMWDAWAAWNPK
                     AAFFAENFLGGNPFTVQLRLGSQPGYPLALPMSLGALGSMAGGWSPILPQLAHASFFL
                     VHWLLFFCIARRGVPAQPVWAGLCAALPALTFQASDQCADFPLSLALAGGTYLVTRPW
                     DSETPILDLWIAGICAGLLPNLKNEGLITGLAFLAFVFWSARSQKWPMRWVFVIVVPV
                     LILWASYFYLRAQSPEAPRFVGDLAAYLRDPARYIAVIKYDLLLLTVVTLLLPVALAM
                     RTRSYAALLPWVGLWAVYSAFFVITTYPQEWHLATAHFRLHLQFLPAFFVVLLLAPIR
                     GKAAENE"
     gene            2377..>2593
                     /locus_tag="PKK76_17440"
     CDS             2377..>2593
                     /locus_tag="PKK76_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70317.1"
                     /translation="MNSRRRDLTAYALMLLIASVVLVFIFITAARTFGADWARVTSFG
                     DFDLSGFRAHLKDSTSILFFQILAILGL"
BASE COUNT          521 a          627 c          684 g          761 t
ORIGIN      
        1 aagcttctga agcttccttt tgtaaaagaa atcattgtag tagatgattg ttcgaaagac
       61 agaacagctg aaattgtaga ggagaagcgg aggcgcaatc gtaagattcg tttacttcga
      121 cactcagaga atcaggggaa gaccgcggct atcaaaaccg cagttcaaga cgcgcaagga
      181 tcgattgtca tcattcaaga cgcggatctc gagtacagcc cttcagaaat gatcaatgtg
      241 gtcagcccaa tcatcaatgg cgttgcggac gtcgtgtacg gaagtcggtt tcttgtgcga
      301 agagaagcgc gtgtgcttta ttatggtcat tttttggcaa atcgctttct aacaacgctc
      361 agcaatatct ttacgaatct caatatgaca gacattgaaa cgggttataa ggccctgcga
      421 actcctttgc tcaagtccct ggttttgacc agtagcggat ttggcatgga agcagaaatc
      481 acggcacaca ttgcgcgttt accaattcgt atttacgaaa ccccgatctc gtactatggc
      541 cgtacgtacg aagaaggaaa aaagattggc atgaaggatg ggatcatggc aattctttat
      601 attttttatt ataatatttt aggagtcaga ctcccatctg cgcgcaaata caggaacact
      661 gtccgcgatt ttctttctga gatcaaaaga tgacctctgg ccgtggggtt tccgtttacc
      721 tgagtctttg cctggccttt ctccttggat tgacctggac tgcagccggg acaattcgag
      781 cggataagca tacacgcatt ctacagacgg cgatcaatga aatgcggagt catcgcggaa
      841 tcaaagctga acgagtggaa ttgtgcagat ctgcgcatca aattgccacc gagttctcag
      901 tggccgcaac ctatgcgatg ccgggcctat cttttgtgga agttgaaaag tgtgcgggtc
      961 cgggtcattc gatccgtttc tttctcacag atgattcaca ggtttcgcca gccatggaac
     1021 atcagattct tttcaaaggg aaggggctgg tgatcgtgga ggccctgtga ttcagtcatc
     1081 gctcaggctc tctctgttca atttggggct gtggttttgt ttgagttatg cacttctggc
     1141 tctggttcct ccagggtcgg tgaatgtctt tcgcgttctt ttcgccgcta ttttcttgat
     1201 cgcgaacttc tttgtggcca ggcggctttc atggaaagaa cccggcgctc ttcgcgaacg
     1261 atcgtggctt gtatttcttc tttgtttcct gtccgcactg attctctttt ttcttagaat
     1321 gtctgcacat ccgcacggaa tgtgggacgc atgggcagcg tggaatccca aagctgcttt
     1381 cttcgcggag aatttccttg gcggaaatcc atttacggtt caactccggc ttggatcgca
     1441 gcctggctat cctctggcgt tgccaatgtc actcggcgct ctcggtagca tggccggtgg
     1501 ttggagtccc attctccctc agcttgcgca tgcttctttt ttcctggtgc actggttatt
     1561 gttcttttgc attgcgcgtc gtggtgttcc tgcgcaacct gtgtgggccg gtttgtgtgc
     1621 tgcacttcca gctttgactt ttcaggcgtc tgatcagtgc gccgatttcc cattgagtct
     1681 ggcactcgcc ggtggcacgt acctggtgac tcgtccctgg gattctgaga ctccgattct
     1741 cgatctgtgg atcgcaggca tctgcgcagg gttgctgccg aatctaaaga atgaaggttt
     1801 gattacgggt ctggcgttcc ttgcttttgt gttctggagt gcgaggagtc aaaaatggcc
     1861 gatgcgttgg gtttttgtca tcgtggttcc cgtactgatt ctgtgggctt cgtatttcta
     1921 tcttcgcgct cagtctccgg aagcaccgcg atttgtgggg gacctggccg cctatcttcg
     1981 ggatccggct cggtacatcg cggtgatcaa gtacgatttg cttcttctca ctgtagttac
     2041 acttttgcta ccggttgcgc tggctatgcg gacgcgcagt tacgcggcat tgcttccctg
     2101 ggtcggcctc tgggctgtct attctgcatt ctttgtcatc acaacgtatc ctcaggaatg
     2161 gcatctggca acggcgcatt ttcgtcttca cttgcagttc ctgccagcgt tcttcgtcgt
     2221 cttgcttcta gcacctatcc gcggcaaggc ggctgagaac gaatagatgc gcgggtgaga
     2281 cataagattg gttatcggaa ccggcatttg tccgggacgc aaatcaagct tgttgggccg
     2341 ctgcgagaca aaaaccatgc gcaaaggtag gcaaacttga actctcgccg gcgtgacctg
     2401 actgcatatg ccctgatgct tctgatcgca agcgttgttc tggttttcat ctttatcact
     2461 gccgcccgga catttggtgc agactgggcg cgcgttactt cttttggcga ttttgatctg
     2521 agcgggtttc gggcgcacct gaaagactca acgagcattc ttttctttca gattctggcg
     2581 attctcggac ttt
//
LOCUS       DAORFF010000565         2588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_28493_length_2588_cov_3.898144, whole genome shotgun sequence.
ACCESSION   DAORFF010000565 DAORFF010000000
VERSION     DAORFF010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28493_length_2588_cov_3.898144"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1012
                     /gene="arsM"
                     /locus_tag="PKK76_17445"
     CDS             149..1012
                     /gene="arsM"
                     /locus_tag="PKK76_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNL70318.1"
                     /translation="MKTSEEIKSMVRETYAGIAQAGPQPSGQSCCGTQKGNVWFNEDY
                     SKLEGYVADADLGLGCGIPVEFSKIKNGDVVIDLGSGAGNDAFIARRQTGETGKVIGI
                     DMTSEMIDRARENNDKLGYNNVEFRLGEIEKLPVSANQADVIISNCVLNLVPDKQAAF
                     LEIFRTLKPGGHFCVSDIVLSGPIPEKLRSVASMYAGCVSGAVQKEEYLNQIKSAGFD
                     NISINKERTIHIPQEVLAEHLTKEEMETLASAKLEVLSITVSGEKPSLKFLQTEKAAA
                     KEACCAPGCCA"
     gene            1000..1464
                     /locus_tag="PKK76_17450"
     CDS             1000..1464
                     /locus_tag="PKK76_17450"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNL70319.1"
                     /translation="MLRITMVKPRILILCTGNSCRSHLAEGILRQAAGDFLEVASAGS
                     KPAGFVHPLAIRAMKEIGIDISEHTSKSMNDFLGSQIETVITVCGNADQACPVFPGQV
                     NRHHWPFDDPAHATGTEEEQMEVFRRVRDEIKKVFEAYADGRRDAIRTGTSK"
     gene            1461..2552
                     /gene="arsB"
                     /locus_tag="PKK76_17455"
     CDS             1461..2552
                     /gene="arsB"
                     /locus_tag="PKK76_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNL70320.1"
                     /translation="MKQEIRHLDFFERYLTIWVLGCMAIGVTLGMLFPEASRTLGALE
                     FTQGSHINAPIAVLIWLMIYPMMLKVDFGGLREVVRKPKGLAITLVVNWLIKPFSMAL
                     LGWIFLHLFFRNLGLMTEEAARDYNAGLIILAAAPCTAMVFVWSYLTNGDSAYTLAQV
                     AVNDLIMLFLFAPIVMLLVGVTGVVVPAQVLIASVVTFIVLPLIAAFASRVFLIRAKG
                     KEWFEQTFLQALKPVTIGALLATLALIFMFQSRNIRENWLAVVLIAVPITIQVYFNAG
                     ITYWLMKIFRVPHNVAAPGSLIGASNFFELAVAVAITLFGTQSGAALATIVGVLVEVP
                     VMLSVCRICVGSRSWYQASTGSPATSVDG"
BASE COUNT          665 a          637 c          657 g          629 t
ORIGIN      
        1 ctcataaaag gcgaaacgga gggcaagacg tcgtgctact gcattgattg gaaggccatg
       61 aaacgattca agtccctcgc ctcgcaattc ttcgaagaaa tggaaaagtt cgaaaaatca
      121 aactgctgct aatgaaagga gaagcaaaat gaaaacgtca gaagaaatca aaagcatggt
      181 tcgcgaaacc tacgcgggaa ttgcgcaggc aggtccacaa ccatccggtc aatcatgttg
      241 cggcactcaa aagggaaatg tctggttcaa tgaagactat tccaaactgg aaggttacgt
      301 tgcagacgca gacctgggtt taggttgcgg aatccctgtg gaattttcga agatcaagaa
      361 cggcgacgta gtcatcgatc taggatccgg cgcaggcaat gacgcgttca tcgccaggcg
      421 ccagacagga gagacgggta aagtcattgg aatcgacatg acgtctgaaa tgattgatag
      481 agcgcgagaa aacaacgaca aactcggata caacaatgta gagtttcgtc ttggagaaat
      541 tgaaaaattg cccgtcagcg cgaatcaggc ggatgtgatc atcagcaatt gtgtgctcaa
      601 tctggttccg gacaaacaag cggccttcct ggaaatcttt cgcacactga aaccaggtgg
      661 ccacttctgt gtgtctgaca ttgttctttc ggggcctatc ccggagaaat tgcgttctgt
      721 agcttcgatg tatgctggat gtgtatccgg tgctgttcag aaagaagaat acctgaatca
      781 gatcaagagc gccggatttg acaatatctc gatcaacaaa gaacgaacca tccacattcc
      841 tcaggaagtt ctcgcagagc atcttacaaa agaagagatg gaaaccctgg cgtccgctaa
      901 actggaagtt ctcagcatta cagtcagcgg tgaaaagcct tctctcaaat tcctgcaaac
      961 agaaaaagcg gcagccaaag aagcatgctg cgcccctgga tgctgcgcat aacaatggtt
     1021 aaaccgcgca ttctgattct ttgcaccgga aattcctgcc gcagtcatct cgcagaaggg
     1081 atcctgcgcc aggcagccgg tgactttctt gaagtggcca gtgccggttc aaaaccggcc
     1141 ggctttgttc atccacttgc cattcgcgcc atgaaagaaa tcggcattga tatttcagaa
     1201 cacacttcga aatccatgaa tgattttctc ggttcacaaa ttgaaacagt cattactgta
     1261 tgcggaaatg cagaccaggc atgtcctgtt tttccaggcc aggtgaaccg tcatcactgg
     1321 cctttcgatg atcctgctca tgccactgga accgaagaag agcagatgga agtgtttcga
     1381 cgcgtgcgag acgaaatcaa gaaagtcttc gaggcatacg cagatggcag acgagatgcc
     1441 atcagaaccg ggacatccaa atgaaacaag aaatcaggca tctagatttt ttcgaacgtt
     1501 atctgaccat ctgggtactt ggatgcatgg caatcggcgt aactcttgga atgctctttc
     1561 cggaagcgtc gcgcactctg ggtgctcttg agttcacgca gggatcacac atcaatgcac
     1621 cgatcgccgt gctcatctgg ctgatgatct atcccatgat gcttaaggta gattttggtg
     1681 gtctacgcga agtggttcgc aagccaaaag ggcttgccat cactctcgta gtcaactggc
     1741 tcatcaaacc attcagcatg gctctacttg gctggatttt cttacatctg tttttcagga
     1801 atcttggtct catgacggaa gaagcggctc gagactacaa tgcaggcttg atcatcctcg
     1861 cggccgcacc gtgcactgcg atggtctttg tctggagcta tcttacaaat ggagactccg
     1921 catacactct tgcacaagtt gcagtgaacg atctcatcat gctgtttctt ttcgcgccca
     1981 ttgtcatgct tctcgtggga gttacagggg ttgtggtgcc agcccaggtt ttgattgcat
     2041 ccgtggtcac tttcattgta ctccccctga tcgcggcgtt tgccagccga gtcttcctga
     2101 tccgtgcgaa aggaaaagaa tggtttgagc aaacatttct acaggctctc aagccagtga
     2161 ccattggcgc acttcttgcc acgcttgcct tgatcttcat gtttcaatca cgcaacatac
     2221 gcgagaactg gctggctgta gtgctgatcg ccgtgccgat cacgattcag gtctatttca
     2281 atgcaggcat aacttactgg ctgatgaaga tcttcagagt gccgcataac gttgccgcac
     2341 cgggttctct cattggcgca agcaacttct ttgagctggc agtggcagtg gccatcacgc
     2401 tttttggaac acaatcaggc gccgctctgg caaccattgt aggtgtgctc gtggaagtac
     2461 cggttatgct ttctgtgtgc agaatctgcg ttggctccag gtcgtggtat caggcgagca
     2521 ctggctcgcc cgcaacgtca gtcgatggat aatccgctgg gcacgcttgc cggcgcactg
     2581 cctgtcaa
//
LOCUS       DAORFF010000566         2580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_28674_length_2580_cov_3.592871, whole genome shotgun sequence.
ACCESSION   DAORFF010000566 DAORFF010000000
VERSION     DAORFF010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28674_length_2580_cov_3.592871"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PKK76_17460"
     CDS             <1..732
                     /locus_tag="PKK76_17460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNL70321.1"
                     /translation="DRRAVARKIGVVFGQKTQLWWDLPVEESFDLLRAVYRIPGAEYK
                     KRLNFFIELLDMKDFLSQQARKLSLGQRMRADLAASLLHRPPILFLDEPTIGLDILTR
                     DIVRKFIKEINEQEKVTILLTTHDIQDIEYLARRLILLDRGRVHYDGGIDDFIQRYAA
                     EKVLSLQVDPPLQSSFFTQRGFEVLSESSDRNFEIRLGADETPAALIEALSAGKIRID
                     EINMRKRDLGDTLKLMYTNQRNVEL"
     gene            732..1517
                     /locus_tag="PKK76_17465"
     CDS             732..1517
                     /locus_tag="PKK76_17465"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNL70322.1"
                     /translation="MAYFKFISMSFQRAISYRVEYFMAVLNAFLYIFIFTSVWRTLVP
                     DGSTKNGLTSAQLTAYAVLSTLVKASYGRNDSLLSTRIRSGEISSDLLKPFHFPLMYL
                     CDTIGASLFQLFSRALPLLVFCVFVFDIRLDMHAETFAKFIPLYLLSFLLFFLMSFLI
                     SSMAFYFTDIFPFWIFYYALITLTSGAIIPVDFFPEEMQKLLYSTPFPYLFYFPTMAI
                     LGRDLVFSYPELVLRYLVLIGSALLLSWMSYRSGIRKLTIAGG"
     gene            1520..2332
                     /locus_tag="PKK76_17470"
     CDS             1520..2332
                     /locus_tag="PKK76_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNL70323.1"
                     /translation="MKKFWNDLPVYGSMIRAAIQSRMEYRGSFIMYILTIMGFYSAQI
                     GVLWVMVNKFNKIGTWNAGEVAFLYSLLVIATGVVSALFSGFLEFSDFVRQGTFDRVL
                     LRPLSPLFSILAMRFEPGGLANIVLGVVAFVTADRLVHIQWSPEAILMLVLVLSGGVM
                     IMGAIRIIIAATAFYTISNEGMQHLFVFSSREFLLYPVDIYNRPVRFALTFLFPLAFI
                     NFYPAHYFLNKSADSLFHPAFVYFTFPVGLVCMIIAIVFWRRAVVRYASTGS"
     gene            2313..>2580
                     /locus_tag="PKK76_17475"
     CDS             2313..>2580
                     /locus_tag="PKK76_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70324.1"
                     /translation="MQAQAVEQTFIETALSLGFDLAAIAPISLPEKDTTAFKDFVAEG
                     RHGTMEYLARNLELRLDPRSLMHDAKSALVLGSFYRTPESDAALK"
BASE COUNT          565 a          599 c          626 g          790 t
ORIGIN      
        1 gacagaaggg cggttgcgcg aaagataggg gttgtgtttg gtcagaagac gcagttgtgg
       61 tgggaccttc cggtggagga aagttttgat ctacttcgcg ctgtgtatcg cattccgggc
      121 gcggaataca agaagcgttt gaacttcttt attgaactgc tggatatgaa agacttcctt
      181 tcgcagcagg cgcgcaagct cagtctgggc cagcgtatgc gagcggatct ggcagcttcg
      241 ctgcttcatc gtccgcctat tttgtttctg gatgagccta cgatcgggct ggacattctc
      301 acacgtgaca tcgtcagaaa attcatcaaa gaaatcaatg aacaggaaaa ggtaacgatt
      361 cttctcacca ctcatgacat tcaggacatt gaatatctgg ccaggcgttt gatccttctg
      421 gatcgcggac gagttcacta cgacggagga atagacgatt tcattcaacg ttatgcggca
      481 gagaaggtgc tgtcccttca ggtggatcct cctttgcaga gtagcttctt tacccagaga
      541 ggatttgaag tgctttcaga atcctccgat cgcaatttcg aaatcaggct gggcgcagat
      601 gaaacaccgg ccgcactcat tgaggcgcta agtgcaggca agattcgaat tgatgaaatc
      661 aatatgagaa agcgggatct gggagacacg ctcaagctca tgtacaccaa tcagaggaac
      721 gtagaactct aatggcttat ttcaagttta tttccatgtc ctttcagcgc gctatcagct
      781 atcgcgtgga atatttcatg gctgttctca acgcctttct gtacatcttc attttcactt
      841 ctgtctggcg cacactcgtt ccggacggta gcacgaagaa tggtctgact tccgcacagc
      901 tcacggccta cgctgttctt tccactctgg tgaaggcaag ctacggtaga aatgactccc
      961 ttctttctac caggattcgc agcggagaaa tttcctcaga tttgctcaag ccgtttcatt
     1021 tccctctgat gtatctgtgc gacacaatcg gcgcgagcct cttccagcta ttttccaggg
     1081 cccttccact tctggtattc tgtgtctttg tatttgatat tcgcctggac atgcacgcag
     1141 agacattcgc caaattcatc ccgctctatt tgttgagttt tctgttgttt tttctgatga
     1201 gttttttgat cagctccatg gcattctatt ttacggatat ctttccgttc tggatttttt
     1261 actacgcatt gatcacactg acgagtggcg caatcattcc agtggatttt tttccggaag
     1321 aaatgcagaa actactttat agcactcctt tcccatatct gttttatttc ccaaccatgg
     1381 ctattctggg aagagatctg gtcttcagtt atccggaact cgttctacgt tatctggtct
     1441 tgattggaag tgctctcttg ttatcgtgga tgtcttatag aagtggcatc cgcaaactta
     1501 ccatcgcagg cggttgaaaa tgaagaaatt ctggaatgat cttcctgtat atggttcgat
     1561 gattcgcgct gcgatccaga gtagaatgga atatcgcggt agttttatca tgtacattct
     1621 taccatcatg ggtttttact ctgcgcaaat tggtgtgctc tgggtgatgg tgaataagtt
     1681 taacaagatc ggaacgtgga atgccggaga ggttgcgttt ctttatagtt tgcttgtgat
     1741 cgcaacgggc gttgtatctg cattgttctc tggatttctt gaattttcag actttgtcag
     1801 gcagggaacg tttgatcgag ttttacttag accactttcg ccgctgtttt ccattctggc
     1861 catgcgattt gagccgggcg gcctggcaaa cattgtgctt ggagtggtgg cctttgtcac
     1921 agcagacaga ctggttcata tccagtggtc gccagaggcc attctcatgc tggttctggt
     1981 gctgtccggt ggagtgatga ttatgggggc cattcgcatc atcattgccg ccactgcgtt
     2041 ttatactata tccaatgaag gaatgcagca tctgtttgta ttttcctcgc gggagtttct
     2101 tctctatcct gtggatattt acaaccgccc ggttcgattc gctcttacat ttttgtttcc
     2161 tctggctttc atcaactttt accccgcgca ctatttcttg aataagagcg cggattcttt
     2221 gtttcacccg gcattcgtat acttcacttt tccagtgggc cttgtgtgca tgatcattgc
     2281 gattgtgttc tggcgacgag ccgtagtgcg atatgcaagc acaggcagtt gagcagacat
     2341 tcatagagac cgctctgtct cttggctttg atctggcggc cattgctccg atttctctgc
     2401 cagaaaaaga caccactgca ttcaaagatt ttgtggcaga aggcagacac gggaccatgg
     2461 agtatcttgc gcgcaatctg gaattgcgcc tggatccacg cagtctcatg cacgatgcaa
     2521 agagtgctct cgttcttggt tccttttatc gcacgcccga atcggatgca gcactgaaac
//
LOCUS       DAORFF010000567         2580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_28677_length_2580_cov_3.246337, whole genome shotgun sequence.
ACCESSION   DAORFF010000567 DAORFF010000000
VERSION     DAORFF010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28677_length_2580_cov_3.246337"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1023
                     /locus_tag="PKK76_17480"
     CDS             67..1023
                     /locus_tag="PKK76_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70325.1"
                     /translation="MGLPGRIVLFVFLAAAAPAGAAPLRISYNLQSTDPTEDQLAALT
                     SYIRDNKGSYFLLQGFACDTGGFRVSLKIASQRQDKIQALIQKEVGRDHTALANPAVI
                     EGEPRIEHRKLEIEAFKTPEALAAAVRLANENTARFHSAFQVETPPETHNEPEDGHPL
                     LWLGLLLAALVIVFIYFILTGNERKRRRHALTPEQAEAVQAITGEAPVQPSQKTRKHR
                     IVTAESVIEPAAFVKELKPMASKKKASKPGPTIKKALDKAYENRSLGEIAKSPVHSLQ
                     GLTPRHSKMLEEAFGVKTVEDLAGLKYFELARAITVLARYED"
     gene            1058..2221
                     /locus_tag="PKK76_17485"
     CDS             1058..2221
                     /locus_tag="PKK76_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70326.1"
                     /translation="MRAFLAILVLGFVNCAKYDAPAGIFGYLLITAAANNHGPCYNPT
                     VKVQTGSTTSSANGTTTVTISAIDTSTSFLIFQATSAGDRPPGATIGGTIASSTTVDF
                     ERFTDGLTTEPAVITIQYYVVTYPSGCASVQRGITNLNADPKNISISSVSSISQAMIL
                     FSRSTGTTENVYDSNNITTAYLTSTSNLRIACAGPSNTNAYWQVVDFPYASIRNQSGS
                     TSILGAATSASVPISSVDLSRSIVLTSLYDSGATGNIGANMVRAYLPDSTTLLIERDD
                     VGAPDNLTRIDYQVIEFQDGTTVQRGNLAFSGGASNATLSLANAVDTSASAPFASFQF
                     GPGQSSGKTPYTANDLVGMATATMTLSSTTLSATRGVTGATADIYAYAVQFGK"
     gene            2271..2537
                     /locus_tag="PKK76_17490"
     CDS             2271..2537
                     /locus_tag="PKK76_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70327.1"
                     /translation="MPQISGNTSDLKPRQLHKLKNLSLRRVRENAVVSQEFARSLCEL
                     SHELSRQIGVLIDRQGHISKILVGDAGRIFIPELPRERSARLRG"
BASE COUNT          715 a          704 c          612 g          549 t
ORIGIN      
        1 atattctgca tctcgttata tggaaaatat cggtcagttt gacaaaacac accgaaaatt
       61 tcaaagatgg gacttcccgg ccgcatcgta ctctttgttt tcctggccgc cgcggctccc
      121 gctggtgcgg ccccgctgcg tatatcctat aacctccagt ctacagatcc aacagaagac
      181 cagctggcag ctctcacgtc ctacattcgc gacaataaag gatcctactt tctactacaa
      241 gggtttgcgt gcgatactgg aggctttcgc gtttcgctca agatcgcaag ccagcgccag
      301 gataaaatcc aggcactcat ccagaaagaa gtagggcgcg atcacactgc actggcaaac
      361 ccggctgtga tagaagggga gccaagaatc gaacatcgca aactggaaat cgaagcattc
      421 aaaacgccag aggccctggc agcagcggta agacttgcaa acgaaaatac agctcgattc
      481 cattcagcct tccaggtaga aactccacct gaaacacata acgagccaga agacggacac
      541 ccgctgctct ggctgggtct actcctggca gcacttgtca ttgtatttat ctatttcatt
      601 ctcacgggaa acgaaaggaa aagaaggcgg catgctctca cgcctgaaca ggcagaggcc
      661 gttcaggcaa ttacgggaga ggctccagtc cagccttcgc aaaaaacaag aaagcaccgg
      721 atcgtcacgg cagaaagcgt gattgaaccc gcagcattcg taaaggagtt aaagcccatg
      781 gcttccaaga agaaagcatc caaaccaggt cctacgatca agaaagcgct ggataaagca
      841 tacgaaaaca ggtccctggg cgaaatcgca aaaagccccg ttcattcgct gcagggtctc
      901 acgccacgac atagcaagat gcttgaggaa gcattcggcg tgaaaaccgt agaggacctg
      961 gcaggcctca aatactttga gctggcgcga gcgatcacag tgcttgcacg atacgaagat
     1021 tgatttacag tctagaaatg caacccatag atatcccgtg cgcgctttcc ttgccatact
     1081 cgttctgggc tttgtcaatt gcgcgaagta tgacgcgccg gcggggattt ttggatatct
     1141 gctgatcacg gcggctgcaa acaatcacgg accttgctac aatccaactg ttaaagtaca
     1201 aacgggctcc accacttcta gcgcgaacgg aaccacaact gtaaccatct ctgccataga
     1261 cacgagcaca tcctttttaa ttttccaggc aaccagtgcc ggagacagac ctccaggggc
     1321 caccataggg ggcacgatcg cttcgagcac tactgttgat ttcgaacgct ttacagatgg
     1381 ccttacaaca gaacccgctg tcatcacaat tcagtactac gtagtaacct atccttccgg
     1441 ttgcgcgtcc gtacaaagag gaattaccaa tctcaacgca gatccaaaga acatctccat
     1501 ctcaagcgta tcctctattt cgcaggctat gattcttttc agcagatcca ctggaaccac
     1561 agaaaacgta tacgacagca acaatatcac gacagcttat ctaacatcta cctcaaatct
     1621 tcgaatcgca tgcgcgggcc cgtcgaacac aaatgcgtac tggcaggttg ttgattttcc
     1681 ctatgcaagc attcgaaatc aaagtggatc cacgagcatt cttggagcgg ccacgtccgc
     1741 ttccgtaccc atcagcagtg tggatctttc caggtccatt gtgcttacga gtctatacga
     1801 ttcgggagcc accggaaaca ttggcgccaa tatggtgaga gcttatttgc ccgattccac
     1861 aaccttgctc attgaaagag acgatgtggg agcgcccgac aacctgacgc gcattgacta
     1921 ccaggtgatt gaatttcaag acgggacaac cgttcagaga ggcaacctcg cattttctgg
     1981 aggcgcaagc aatgcaactc tgagcctggc aaatgcggtg gatacgtctg catccgctcc
     2041 attcgcatcg tttcaatttg gaccgggaca gagctctggt aaaacaccct atacggcaaa
     2101 cgatctagtt ggaatggcaa ctgcaacgat gacactgagt tccacaacgc tcagtgcaac
     2161 cagaggagtc actggagcca cggcagacat atacgcatac gcagttcaat ttggcaaatg
     2221 acgagtgaca atccagcggg gcatgcttat attgttcgaa tcagacaagc atgccgcaaa
     2281 tttcaggcaa cacttccgat ctcaaaccgc gccagcttca taaactgaag aatctatctc
     2341 tccggcgcgt acgcgaaaac gctgtagtca gtcaggaatt cgcgcgaagt ctctgtgagc
     2401 tttcgcacga actctccaga caaattggag tgctcataga tagacaggga cacatctcga
     2461 agatacttgt gggagacgca gggcgcatct tcatcccgga attgccccgc gaaagatccg
     2521 ccaggctgcg cggctgagac tcgttgatct tgcccgccag ttcgatgaac tcggggtaga
//
LOCUS       DAORFF010000568         2569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_28893_length_2569_cov_3.404932, whole genome shotgun sequence.
ACCESSION   DAORFF010000568 DAORFF010000000
VERSION     DAORFF010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28893_length_2569_cov_3.404932"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /gene="trpS"
                     /locus_tag="PKK76_17495"
     CDS             complement(<1..611)
                     /gene="trpS"
                     /locus_tag="PKK76_17495"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNL70328.1"
                     /translation="MRVLSGIQPSGRLHLGNYFSMMKRMIDYQANADLFCFIASYHAM
                     TTVQEGPALREGIHQAAVDFLALGLDPDKCTFWVQSDVHEVTELAWLLSTSITVSQLE
                     LAHSYKDKIAQGIIPSAGLFTYPVLMAADILLFGSNRVPVGKDQKQHLEITRDICHRF
                     NFSKESFERRRILERRGSLERREVLGRRGVFEIYIQENDKKNT"
     gene            696..1082
                     /locus_tag="PKK76_17500"
     CDS             696..1082
                     /locus_tag="PKK76_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70329.1"
                     /translation="MDLGFALLFLLAAVLIGGLIWMLFNLRDYGIDTTPSDPIRGYCP
                     ICAAGLRKGEKIRSSVVEIGNVEIQTRIKGCPYCLGEKSTRKRTCPVCKANLPVDQLI
                     LAISDPRVDKRKLAIRGCKTCYPQGF"
     gene            1107..2249
                     /locus_tag="PKK76_17505"
     CDS             1107..2249
                     /locus_tag="PKK76_17505"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNL70330.1"
                     /translation="MAIRFLCAPNAFKESIPAVDAALAMKRGILRVFPDAVVECIPIA
                     DGGDGTAKALSTAMPDAKIEHVTVKDPLGRMRDSQFVIGTLNKVRTAIVEMALASGLA
                     LVTEEERDVMRSSSAGTGELIRAALDAGAVQILIGLGGSATNDCGAGLLQELGAKLLD
                     SHGQSIGPGPSGLEHLTAMDLSTLDDRLGPNASVKLLVMCDVQSPLTGKTGASFVFGP
                     QKGARIEDLEHLDKLLEKTGRLLEKECNRSLIHTPGSGAAGGTAAALLAVGARLTPGF
                     QMISELLGVERAIKQSDYVFTGEGKFDSQSEEGKGPSEILKLARAHKKECFVLAGSVE
                     GDAENVFQIAGPNISRAESMRRASELIEDTARKVCEQIRTRTGTDS"
     gene            complement(2252..>2569)
                     /locus_tag="PKK76_17510"
     CDS             complement(2252..>2569)
                     /locus_tag="PKK76_17510"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNL70331.1"
                     /translation="MDYNGIQIDGFTRDVCDLGDLEKKFGSFGWQVETRNGHSIPEIR
                     EALGSAAERLRKNPNVGPQMVLFKTVLGKGVSYMENVPGWHGKSPNDEEFKRAMRDLG
                     VAV"
BASE COUNT          684 a          667 c          634 g          584 t
ORIGIN      
        1 attgtatttt tcttatcatt ttcttgaatg tatatctcaa aaactcctct tcttccaaga
       61 acctctctcc tctcaagact ccctctcctc tcaagaattc ttcttctctc aaaagactct
      121 tttgagaaat tgaaacgatg acagatatca cgcgtgattt ccagatgttg cttctggtct
      181 tttccaacag gcacccggtt ggatccaaat aacagtatgt ctgccgccat aagaacggga
      241 tacgtaaaca agccagcgga aggaatgatg ccctgcgcaa ttttatcttt gtaagaatga
      301 gcaagctcca gttgcgaaac tgtgatgctc gtggaaagaa gccacgcaag ctctgttact
      361 tcatgcacat cgctttgaac ccagaaagtg catttgtccg gatcaagacc caatgccagg
      421 aaatccacag ctgcctgatg aatcccttcc ctcaaagcag gaccttcctg cacagttgtc
      481 attgcatggt agcttgcaat gaaacaaaac aaatccgcat tcgcctgata gtcgatcatt
      541 cgcttcatca tggaaaaata gttcccgaga tgcaaacgac cgctcggctg gatgcccgac
      601 aaaactcgca tgatccgaat tcgcgcgacc agacagggcg aaaagcattt tgcaaatgtg
      661 ctttgcacac agggcagaga aattagtcga tgagcatgga tctcgggttc gctctgttat
      721 tcctgctggc cgcagtcctc attggcggac tcatctggat gcttttcaac ctgcgcgact
      781 acggaatcga caccactcca tccgatccaa ttcgcggcta ctgcccgatc tgcgcggctg
      841 gactgagaaa gggcgaaaaa atccgctcgt ctgtggtaga aattggaaac gtcgaaatac
      901 aaacccggat caagggctgc ccgtactgcc ttggagaaaa atcgaccaga aaacgaacgt
      961 gcccggtctg caaagcaaac ctgcccgtag accagctcat ccttgccatt tcggatccaa
     1021 gggtagacaa acgcaagctg gccattcgcg gctgcaaaac ctgctatccc cagggattct
     1081 aaattcatac gtagtactga ataataatgg cgattcgatt tctatgcgca cccaatgcat
     1141 ttaaagaaag catcccggcg gtagatgcag ctctggccat gaagcgcggt attttacgag
     1201 tatttccgga tgcagtcgta gaatgcatcc ccatcgcgga cggcggagac ggaaccgcaa
     1261 aagcactatc gactgcaatg ccagacgcaa agatcgagca tgtgactgtc aaagatccac
     1321 tcggtagaat gcgcgattca caattcgtga ttggaacgct aaacaaagta aggacagcga
     1381 tcgtagagat ggcgctcgca tccggtcttg cactcgttac agaagaagaa agagacgtta
     1441 tgcgatcttc ttctgccggt acaggtgaac tcatccgcgc tgccctggac gcgggagccg
     1501 tgcaaatcct catcggtctg ggtgggagtg cgacgaatga ttgcggagca ggcttgcttc
     1561 aggaactggg cgcgaagctt ctggattcac acggccagtc catcggaccg ggtccgtctg
     1621 gtctcgaaca cctgacggca atggatcttt ctactctgga cgatcggctc ggaccgaatg
     1681 cgagcgtgaa actactggtt atgtgcgatg tacagagtcc tcttaccgga aaaacaggag
     1741 catcttttgt gtttggacct caaaaaggcg cgcgcataga agatctagaa cacctggata
     1801 agctcctcga gaaaactggc agattgcttg agaaagaatg caatcgatct ttgattcaca
     1861 cgcctggatc gggcgcagcc ggaggaacag cagccgcgct tcttgccgtt ggggcgcgac
     1921 tgactcctgg cttccagatg atttcagaat tgcttggcgt ggaacgcgcc ataaagcaaa
     1981 gcgactacgt tttcacggga gaaggcaaat tcgattcaca atcggaagaa ggaaaagggc
     2041 catctgagat actgaaactg gcgcgcgcgc acaaaaaaga atgctttgtt ctggcaggtt
     2101 cagtagaagg cgacgcggag aatgtctttc agattgccgg tccaaacatc agtcgcgcag
     2161 aatccatgcg aagagcatct gaacttatag aagacacagc acgcaaagtg tgcgagcaaa
     2221 tccgcacaag aaccggcacg gattcctaac gttagacggc gacgcctaga tctcgcatgg
     2281 cacgtttgaa ctcttcgtcg ttgggtgact taccatgcca gcctggcacg ttctccatat
     2341 aggaaacgcc tttgcccaga accgttttga aaagaaccat ttgaggacca acgtttggat
     2401 tcttccgcag acgctctgca gcagagccaa gtgcctcacg aatctcaggg atgctgtgcc
     2461 cgtttcttgt ttctacctgc cagccaaaag aaccaaactt cttttccaga tcgcccaggt
     2521 cgcagacgtc gcgagtgaat ccgtcaatct ggatgccgtt gtagtccat
//
LOCUS       DAORFF010000569         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_29091_length_2559_cov_3.258387, whole genome shotgun sequence.
ACCESSION   DAORFF010000569 DAORFF010000000
VERSION     DAORFF010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29091_length_2559_cov_3.258387"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PKK76_17515"
     CDS             <1..376
                     /locus_tag="PKK76_17515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387818.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNL70332.1"
                     /translation="LAQFRDLEAFAQLGTELDAATQAQLDRGYRLLEILKQEENSPWP
                     VEEQVASIFAITRGLMDKIPVNRVREFEKGLIDHLRKAHNEFLKGIREKKAVEDEKAL
                     ERCIADYATSFLKDQAAEIRPH"
     gene            389..1294
                     /gene="atpG"
                     /locus_tag="PKK76_17520"
     CDS             389..1294
                     /gene="atpG"
                     /locus_tag="PKK76_17520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNL70333.1"
                     /translation="MAGAKVLKKRIGAVKNTRKITRTMEMVSTAKAKRMVDRVNAARP
                     YNEKIVEIMKSMESVVGEGAGVLPLLRQVEHPHRVALLVVTANRGLCGGYNASVLRMA
                     RARLVEYQKQGKEVQLFVIGKKGNSYFKFLKVPVKKVITDVDDTFQYDQAKHLSDELI
                     SGFAGAHFDRVEVISTVYYSSALQKPRLDVLLPVGAHTEEDEAELAKKKLTEPVNRAT
                     FLFEPAPAFILKRILPHVIRATVFRKILESVTSEQIARRIAMKNATDNAGSMTKSLTR
                     IYNRKRQAGITQELAEIVSGADAIG"
     gene            1322..>2559
                     /gene="atpD"
                     /locus_tag="PKK76_17525"
     CDS             1322..>2559
                     /gene="atpD"
                     /locus_tag="PKK76_17525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNL70334.1"
                     /translation="MAKQAATKTKAKSGKILQVIGSVLDISFDAGELPDIYTALEIDV
                     PGPDGKKETLTAEIQQHLGGNVCRAVALSSTDGLVRGQEVRNTDAPISVPVGQVTLGR
                     IFNVLGETVDEGPAVQVTERRAIHQKAPDLQNLDSRTEIFETGIKVIDLLAPYTRGGK
                     TGLFGGAGVGKTVVIMELINNIAKAHGGYSVFAGVGERTREGNDLWLEMTESGVLHKT
                     VLCYGQMNEPPGARLRVALTALTMAEHLRDSTGTDCLLFVDNIFRFSQAGSEVSALLG
                     RMPSAVGYQPTLATEMGLLQERITSTRTGSITSVQAIYVPADDLTDPAPATAFTHLDA
                     TTVLSRAISEKGIYPAVDPLDSTSRALQPGVVGEEHYKTAREVQRVLQRYKELQDIIA
                     ILGMDELSEEDKILVGRARK"
BASE COUNT          658 a          621 c          681 g          599 t
ORIGIN      
        1 cctggctcag ttccgcgatc tagaagcgtt tgcacagctt ggaacagaac tggatgccgc
       61 gactcaagcg cagctcgatc gcggttatcg cctccttgaa atcctcaagc aggaagaaaa
      121 cagcccgtgg ccggtagaag agcaggtagc ttctattttt gcgattacca gaggtctcat
      181 ggacaaaatt ccagtgaacc gcgttcgcga atttgaaaaa ggtctcatcg accatctcag
      241 aaaagctcac aacgaattcc tgaaaggcat tcgtgagaag aaggctgtgg aagacgagaa
      301 agcactcgag cgttgcatcg cagactacgc cacgtctttc ttgaaagacc aggcagctga
      361 gattcgtccg cactaatcgg gaaagagaat ggctggcgcg aaagtactta agaagcgaat
      421 cggcgcggta aagaatacgc gcaagatcac gcgaaccatg gaaatggttt caactgcgaa
      481 agcaaagcgc atggtagacc gtgtcaatgc tgctcgccct tacaacgaga agatcgtgga
      541 gattatgaaa tccatggaat ccgtcgtggg ggaaggtgca ggcgttcttc ctttgctgcg
      601 tcaggtggaa catcctcatc gcgtggctct gcttgttgtg actgcaaacc gcggtctctg
      661 cggcggatac aatgcgagcg ttcttcgcat ggcgcgtgct cgtctggtgg aataccagaa
      721 gcagggaaaa gaagttcagc tctttgtaat tggtaagaaa gggaattcct acttcaaatt
      781 cctgaaagtg cccgtgaaga aagtcattac tgatgtggac gataccttcc agtacgacca
      841 ggcaaagcat ctttcggatg aactgatctc tggctttgca ggtgcgcatt ttgatcgtgt
      901 ggaagtcatt tctactgtct actatagctc tgccctgcaa aagccgcgtc tggatgttct
      961 tcttccagtc ggcgctcaca cagaagaaga tgaagcggag cttgccaaaa agaaactgac
     1021 agagccagtc aatcgtgcta cgtttctgtt tgaaccggca cctgccttta ttttgaagcg
     1081 aattctgcct cacgtaattc gtgctacggt gtttcgcaag attctagaat cagtgacttc
     1141 agaacaaatt gcgcgtcgca ttgctatgaa aaacgcaacg gacaatgcag gttccatgac
     1201 caagtcactg actcgcattt acaaccgcaa acgtcaggcg ggcattacac aggaactggc
     1261 agagatcgta tctggtgcag acgcgatcgg ttaagcaatc ataagtcaag aggaagaaaa
     1321 aatggccaag caagctgcaa caaagacaaa agcaaaaagc ggaaagatcc ttcaggtgat
     1381 cggttcggtt ctggatattt cattcgatgc gggtgaactg ccggatatct ataccgcact
     1441 cgaaatcgat gtgccgggtc cggacggaaa aaaagaaact ctgaccgctg aaatccagca
     1501 gcacctgggc ggaaacgttt gtcgtgcggt tgcactttct tccacggacg gactggtgcg
     1561 cggacaggaa gttcgcaaca cagacgctcc catttctgtt ccagtagggc aggtaactct
     1621 ggggcgtatc ttcaacgttc tcggggaaac agtagacgaa ggtccagcgg tgcaggtgac
     1681 ggaacgccgc gcgattcacc agaaagctcc tgatctgcaa aacctcgatt ctcgcactga
     1741 gatctttgaa actggaatca aggtcatcga ccttcttgct ccgtatactc gcggtggtaa
     1801 gaccggtctc ttcggcggtg cgggtgtggg caaaaccgtc gtgatcatgg aactcatcaa
     1861 taacatcgcc aaggcgcacg gtggttactc cgtattcgca ggtgtaggtg aaagaactcg
     1921 tgaaggaaat gacctgtggc tcgaaatgac tgagtcaggc gttcttcaca aaacagttct
     1981 ctgctacggc cagatgaatg agccgccagg tgctcgtctt cgcgttgctc tgacagctct
     2041 gaccatggca gaacacctca gagatagtac tggaacagac tgcctcttgt ttgtggataa
     2101 catcttccgt ttctcgcaag cgggttctga agtatctgca ctcctgggtc gtatgccatc
     2161 tgctgtagga taccagccaa ctcttgctac ggagatgggc cttctacaag agagaattac
     2221 atctactcgc accggttcga ttacgtctgt tcaggcgatt tatgttcctg ctgacgactt
     2281 gactgacccg gcgcctgcaa ctgcgtttac tcacctggat gcaactacgg ttctttctcg
     2341 tgcgatttct gaaaaaggaa tttatcctgc tgtggatccg ctcgattcaa cttctcgtgc
     2401 tctgcagccg ggtgtggtgg gcgaagagca ttataagact gcgcgcgaag tgcagcgtgt
     2461 tcttcagcgt tataaggaac ttcaggacat catcgcgatt ctgggtatgg acgaactctc
     2521 agaagaagac aagattcttg tgggccgcgc tcgcaagat
//
LOCUS       DAORFF010000570         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_29133_length_2557_cov_3.223821, whole genome shotgun sequence.
ACCESSION   DAORFF010000570 DAORFF010000000
VERSION     DAORFF010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29133_length_2557_cov_3.223821"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..701)
                     /locus_tag="PKK76_17530"
     CDS             complement(3..701)
                     /locus_tag="PKK76_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004502972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNL70335.1"
                     /translation="MDRSRKFTFKERIQIAVIPRLVVWFQKLIGLTSKRINLGGEHFD
                     SLHGANKPWIFAIWHTNVLYSPYLLRDLNTAVMISASRDGEFIESVVRQFGNTSIRGS
                     TSRGGGAALKEMIGHLRKGFSAAITPDGPRGPALVVQPGLIMSAQLSQVAILPFHYEC
                     TRQKIAKSWDAHRLPLPFTTFVCSYGEPILIPRQLSPEEFEAQRLKVERAMLENRERC
                     IAEAKRLARPLEQT"
     gene            complement(722..803)
                     /locus_tag="PKK76_17535"
     tRNA            complement(722..803)
                     /locus_tag="PKK76_17535"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(767..769),aa:Leu,seq:taa)
     gene            complement(812..883)
                     /locus_tag="PKK76_17540"
     tRNA            complement(812..883)
                     /locus_tag="PKK76_17540"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(848..850),aa:Cys,seq:gca)
     gene            complement(930..>2557)
                     /locus_tag="PKK76_17545"
     CDS             complement(930..>2557)
                     /locus_tag="PKK76_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70336.1"
                     /translation="QVLQTIADLWKKDRAKMLESADSLTGFLQTRENQTGNIPDSGVF
                     AQAASHFRNSYDELRGGFLSNGPNKFPPSLNMLFLLRHFEHTGDESILAMVENTCDAM
                     KRGGIYDQIGGGLSRYATDHDWLVPHFEKMLYDNALFARVLTETYRITGNERYKTWAL
                     DIFEYVARDLTSPDGAFYSAEDADSEGKEGLFYLWSADEIDSLLKEHSFSAETRKQIA
                     RFYGVSMRGNFEGKNILHEASVREEFLRAHNLKAAIWDETIGKVRHLLLLERSKRIRP
                     LRDDKVLTAWNALMVSALARAGAVFGDAGLTARAEKAAAFIESHMVQGETILRRFREG
                     EARFEGSLQDYAGLALSFIDLYQTTFRAHYITRAMQIAQVIATKFKAEGGGFFDTADG
                     IPDLLVRSMEIYDGVEPSGNALAAMLFGTLARYGYENCGSVAEEILARFGGEVATHAP
                     AHCMMLLGLDLYLNPPHEVAIVDGSDALGDSILAELRPRLSIGSIIARGEDQSIPLLK
                     SRKAEGGKTTVYVCRNFVCDRPVHSAAEALALL"
BASE COUNT          587 a          644 c          682 g          644 t
ORIGIN      
        1 tactacgttt gttcgagtgg ccgcgcgagg cgttttgctt cggcgatgca tctttcgcgg
       61 ttttcaagca tcgcacgttc cactttgagt ctttgggctt caaattcctc aggggaaagc
      121 tgccgaggaa tcaggattgg ttctccgtaa gagcaaacga aagtagtgaa gggaagaggc
      181 agacgatgcg cgtcccagga tttcgcaatc ttctgtcgcg tgcattcgta atgaaaaggc
      241 aaaatcgcca cttgcgaaag ctgtgcggac atgatgagac ctggctgcac gacgagtgcc
      301 ggtccacgcg ggccatccgg agtgatggcg gcagagaatc ctttgcgcaa atgaccgatc
      361 atttctttga gagcggcacc gcctccgcgc gaagtggatc cgcggatgct tgtgtttccg
      421 aattgtctga ctacggattc aatgaattct ccgtcgcgcg aagcagagat catcacagca
      481 gtgtttagat cgcgcaaaag atacggagaa tacagaacgt ttgtgtgcca gatggcaaag
      541 atccagggct tatttgcgcc gtgaagagag tcgaagtgct ctccacccag attgatgcgt
      601 ttggaagtaa ggccgatcag tttctgaaac cacactacga ggcgtggaat caccgcgatc
      661 tggatgcgtt ctttgaatgt gaactttcgg cttctgtcca ttcagtttcc atccgcgaca
      721 ttacccggag cgggacttga acccgcacag ccgaagccgc aggattttaa gtcctgtgtg
      781 tctaccgatt ccaccatccg ggctaaagat gaggcgtcgc cgggatttga acccgggatc
      841 aggcttttgc agagccttgc cttagccact tggccacgac gccaggaagg ctccagtctt
      901 gaaatcagag gaccagggtc aatttgtttt tacagaagtg caagagcctc tgctgcggaa
      961 tgcactggac gatcgcatac gaagtttctg caaacgtata cagttgtttt tccgccttct
     1021 gctttgcgcg acttcaaaag gggaatgctc tggtcctctc cgcgcgcgat gatcgatcca
     1081 atgctcagtc tgggtctcag ttcagcgaga atcgaatctc ccagagcatc acttccgtct
     1141 acaatggcga cttcatgcgg cggattgaga tatagatcga ggccaagaag catcatgcaa
     1201 tgagccggtg cgtgagttgc tacttcgccg ccaaagcgtg ctagaatctc ttctgcaaca
     1261 ctgccgcaat tttcgtaacc gtagcgcgcc agggttccaa agagcatggc tgccagggca
     1321 tttccggaag gttccactcc atcatagatt tccatagagc gaaccagtag atctggaatt
     1381 ccatccgcag tgtcaaagaa gccaccgccc tccgctttga atttagtagc aatgacctgt
     1441 gcgatttgca tcgctcgcgt gatgtaatgg gcgcgaaagg tggtttgata aagatcgatg
     1501 aaggagaggg caagtcctgc gtaatcttga agagatcctt cgaatctggc ttctccttct
     1561 ctgaatcgcc tcaggatggt ttctccttgc accatgtgcg attcaatgaa cgcggctgct
     1621 ttctctgcgc gcgcagtgag tcctgcgtct ccaaacactg caccggcgcg cgcgagtgca
     1681 gataccatca gagcgttcca ggcagtcagt accttatcgt cgcgcagagg tcgaattcgt
     1741 ttggaacgtt ccaatagcaa aaggtgtctg acttttccga tcgtctcgtc ccatatcgcg
     1801 gcttttagat tgtgcgctct aagaaattct tcgcgaacgg acgcttcgtg caaaatgttt
     1861 ttgccttcga aattcccgcg catgctcacg ccgtagaacc gtgcgatttg cttgcgagtc
     1921 tctgctgaga aagagtgctc tttgaggagt gagtcgatct catctgcgct ccacagatag
     1981 aaaagccctt cttttccctc actgtctgcg tcttctgcag agtaaaacgc gccatccgga
     2041 gaagtaagat cgcgcgccac atattcgaat atatccagag cccaggtctt gtagcgctca
     2101 ttcccggtga ttctataggt ctccgtgaga acgcgtgcaa aaagagcatt gtcataaagc
     2161 atcttttcaa agtgtggaac cagccagtcg tgatcggttg catatcgtga gaggccgccg
     2221 ccaatttgat cgtaaatgcc accacgcttc atcgcgtcgc atgtattctc caccatggca
     2281 agaatcgatt cgtctccagt gtgttcgaag tggcgcagta gaaagagcat gttgaggctg
     2341 ggcggaaatt tgtttggtcc gttggataga aagccgccgc gcaattcatc gtagctgtta
     2401 cgaaagtgag aagctgcctg tgcgaaaacg cctgaatccg gaatgttacc ggtctgattt
     2461 tcgcgcgttt gaaggaatcc ggtcaaggaa tctgcggatt cgagcatttt tgctctgtct
     2521 tttttccaga gatctgcgat ggtttgaagt acctgta
//
LOCUS       DAORFF010000571         2555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_29181_length_2555_cov_3.220400, whole genome shotgun sequence.
ACCESSION   DAORFF010000571 DAORFF010000000
VERSION     DAORFF010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29181_length_2555_cov_3.220400"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PKK76_17550"
     CDS             complement(<1..669)
                     /locus_tag="PKK76_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70337.1"
                     /translation="MSERLYFPTFTRHLSILLVVQSLAGSTSAVAFLVSGFAGTSLAP
                     VRILATLPMSAFVIGVAISSSLAAWTMSRAGRKTGHLIGASIGFSGAACACVALTIHS
                     FWGYSASCVMLGVGTAFNNQVRFTAAEGAPPAQKGLVHSWILMCGIFAAILGPAVAAY
                     GRKMDLFGGAAEYAGSYVLLMVMLLASISLLAFLPSLGTTTQTASDSGKISFKGEDIT
                     ALPAF"
     gene            complement(674..1648)
                     /locus_tag="PKK76_17555"
     CDS             complement(674..1648)
                     /locus_tag="PKK76_17555"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HNL70338.1"
                     /translation="MHLEERKVLLNRASIRGICRGWRDKEWWYTGVLDPESQVYVSWY
                     FLRVNVADAFVFSVFDPSRPGPEHLVKQIFYLEKPPESSREGTYLVRDRRGFFVSYTG
                     RGDGPEPGWSFLLKTKSIEAEIRIEPRRPSFTKFDNAVQDNYGLLNFFGASASGKVQA
                     GGHTYELNSAVAYYDHCFGRIPEQTGWHWIAVQGDKFSLDSLINYGAYAQCYTQGWHS
                     IEGVPRPGQWVRLEQAVSFENSVSFDDKDYLSWKKPWRLTSTDLDLNIEILQHKTDLT
                     RVPPILPFLVKLHHTEAFVRVSGKARIDGVWTQTGDMFGVFEQHYGRW"
     gene            1729..>2555
                     /locus_tag="PKK76_17560"
     CDS             1729..>2555
                     /locus_tag="PKK76_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70339.1"
                     /translation="MTTCTRLAAIPGALVALFLTTQCGAPVEKLGASKGSARISIRDG
                     SIVTLDGEWEAAWNQIIPPDSAHHGSSFFKLPGYWNDVEPLAAGQPSGHRYATFILRT
                     KGDLPPDTAVYIKEARTAYALYCAKNLIASTGRVGSTWEPDMADLTPRLGKLPTDCLR
                     DPEFTLQVSNFDYSLGGPGSPIRIGNHDLLRIAQIRRDALSFALAGILFVMTLYHIVW
                     WLVRIQDLRNIWFSALSFLCMLRIIGTENAIPRLLGPGTFEMQMKVEFLAFYLMIPVS
                     "
BASE COUNT          651 a          705 c          636 g          563 t
ORIGIN      
        1 gaaggccggc agcgcggtga tgtcctcccc cttgaagctg atcttcccgg aatctgatgc
       61 cgtttgcgtg gttgttccga gagacggaag aaatgcgaga aggctgatcg atgccaaaag
      121 cataaccata agtaatacgt atgaacctgc atactctgcc gcgcctccga agagatccat
      181 ctttctgcca tacgctgcca cagccggccc aagaatggct gcaaagatcc cgcacatcag
      241 aatccaggaa tgcacaagac ccttctgcgc tggaggcgcg ccttccgccg cggtaaacct
      301 gacttgattg ttgaaggctg ttcccacgcc gagcatcacg cacgaggcag aataccccca
      361 gaacgaatga atcgtcagtg cgacgcaagc gcaggctgct ccagaaaatc caatgctcgc
      421 gccaatgaga tgcccggttt ttctaccagc cctggacatg gtccaggcag ccagagaact
      481 ggagatcgcc accccaatga caaacgcgga catgggcaga gtagccagga ttcttacagg
      541 agctagactg gttcctgcaa atccgctcac gagaaacgca accgcagaag tagagccggc
      601 cagagattga actacaagca ggatgctcag gtgccttgtg aaagtgggaa agtacagtcg
      661 ttcgctcaag gcatcaccat cttccgtagt gctgctcaaa gacgccaaac atgtctccgg
      721 tctgtgtcca cactccgtct attctagctt tgcccgaaac tcgaacgaaa gcctccgtgt
      781 gatggagttt caccaaaaac ggaagtatgg gcggaacgcg cgtaaggtca gtcttgtgct
      841 gcaatatttc aatatttaaa tccaggtccg tggacgttag gcgccatggc ttcttccagg
      901 acagatagtc tttatcatca aaagaaacac tgttttcaaa agagactgcc tgctccagac
      961 gcacccactg gccagggcgt ggaacacctt cgatcgaatg ccatccctgc gtataacact
     1021 gcgcatacgc tccgtagtta atgagcgaat ccagagaaaa cttatctccc tgaacagcga
     1081 tccagtgcca cccggtctgt tcgggaatcc ggccaaagca atgatcatag tacgcaacag
     1141 cagaattcaa ttcatatgtg tgtccgccag cttgcacttt tccggatgca ctggctccaa
     1201 aaaaattcaa cagaccatag ttgtcctgga cggcgttgtc gaatttggtg aatgaaggtc
     1261 ttcgaggttc aatgcgaatc tccgcctcaa tcgattttgt tttcagtaag aacgaccagc
     1321 cgggttctgg accgtctccg cggccagtgt aagatacaaa aaaaccacgc ctgtcgcgaa
     1381 ccaggtatgt tccttctcta gacgattcag gtggtttttc taaatagaag atctgcttga
     1441 ccaggtgctc tggcccaggc cgcgaaggat cgaaaacgga aaatacaaac gcatccgcga
     1501 cgttgacgcg caaaaaatac cagctgacat acacttgaga ctctggatcc aaaacccctg
     1561 tataccacca ctctttgtcg cgccatcccc tgcagattcc tctgatgctc gcacggttca
     1621 gaaggacctt tctctcttcc agatgcacgg ggcagtgcgc cccgggagag aagaactgca
     1681 atcccaaaaa atgctgggtt cgccagcgcg ctgtttcaat atccgtcaat gacgacgtgc
     1741 accaggctcg cggcgattcc aggtgcgctg gtagcattgt ttctcacaac tcaatgcggc
     1801 gcaccggtgg aaaagctagg ggcaagcaaa gggtcagcaa gaatttccat ccgggatgga
     1861 agcattgtaa ctctggacgg agaatgggaa gcagcctgga atcagatcat tcctccagac
     1921 agtgcgcacc acggatcttc gtttttcaag ctacctggat actggaatga cgtggaacct
     1981 cttgcggcgg ggcaaccttc cggccaccgt tatgcgacat tcattctgcg aacaaaagga
     2041 gatctgccgc cggacaccgc agtttacata aaggaagcaa gaactgcgta tgcactttac
     2101 tgcgcaaaga atctaatcgc aagtaccgga agagttgggt ccacgtggga gccggatatg
     2161 gcagatctca ctcctagact gggtaaactc ccaacagact gcctcagaga ccctgaattc
     2221 acactccagg tgagcaactt cgactattcc ctcgggggcc cgggttctcc tattcgcatc
     2281 gggaatcatg acctgcttcg catagcacag attagacgag acgcgctgag tttcgcactg
     2341 gctggcatcc tgtttgtcat gacgctctac cacattgtat ggtggcttgt gcgtattcaa
     2401 gacctgcgca acatctggtt ttcggcactt tcctttcttt gcatgctgcg catcatcggg
     2461 acagaaaacg caattcctcg ccttctcggg cccggcacat ttgagatgca aatgaaggtg
     2521 gaattcctgg ccttctatct aatgattccc gtttc
//
LOCUS       DAORFF010000572         2553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_29217_length_2553_cov_3.730985, whole genome shotgun sequence.
ACCESSION   DAORFF010000572 DAORFF010000000
VERSION     DAORFF010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29217_length_2553_cov_3.730985"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="PKK76_17565"
     CDS             <1..96
                     /locus_tag="PKK76_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HNL70340.1"
                     /translation="SDVENLMEITKLSSVFEIFKTVEEALESFDF"
     gene            complement(116..952)
                     /locus_tag="PKK76_17570"
     CDS             complement(116..952)
                     /locus_tag="PKK76_17570"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNL70341.1"
                     /translation="MKQPVTKIFVAINVLVHLYVWWYVQGTQGPQAADALIDRFGLTP
                     AEFRNGAFWQPLTALFLHDGRVFPIIHIGFNMLAIWSFGLALENTLGSIRFAWLMFVS
                     GCASSLFVILFQADLPNPTIGASGIVLGLLGALAVFYPNSQMLIFFIPMRARNAAILI
                     GVLSIVLPMFGVLTFISHMGHLGGLVGGVLYSWFALGLRFGQNDLRERESYIRRKVQE
                     QEYLREMLQRLQKASPRFQGTSRERWKEPDEKVINPRPEDVQGPEKRLYYDPTTGKFY
                     TR"
     gene            complement(965..1927)
                     /locus_tag="PKK76_17575"
     CDS             complement(965..1927)
                     /locus_tag="PKK76_17575"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70342.1"
                     /translation="MKLRWRLEVGLASAVFRFFGLWSGAGRIMCGRLFGFLGRILVAK
                     VRKRTRANLRIAFPEASEARIDQLMRDAYCSIAKGSYDFYWSGRWTKEEVESRIQWEP
                     ESLKSFESAYRAGKGIVLVVGHLGNWEFVSHAIMLRGMQLNAYAAIQVNTLANEMVEK
                     IRKRFGAKFFAPRMMNSPDMLRCLKKGEIAGFVADQNAGSRGFFVPFMGQLASTHKEP
                     ASYAALSGSEAFFGVGLDQGQGVYRIEMIPLGRCGRSESEILAFTARWVDILEKYIRM
                     YPEQYFWMHNRWKSRPGPDSIIVERAKSDLQPREKNAVGVERKP"
     gene            1978..2379
                     /locus_tag="PKK76_17580"
     CDS             1978..2379
                     /locus_tag="PKK76_17580"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNL70343.1"
                     /translation="MPGRDPSGGFGSGVDNEDGLQLKLTFDEDTKTAYGEYRCPAKFR
                     GEKESVHPGVLAVILDEIMARINEAMNFDTVTGELTIRYLQSATVDENLYLRGWFVKK
                     NRRVVENRAEIENEIGKIVARGKGKYIERED"
BASE COUNT          684 a          675 c          596 g          598 t
ORIGIN      
        1 tcagatgtcg aaaacctgat ggagattacg aaactcagtt cagtgtttga gatcttcaag
       61 accgtagagg aagcgctcga aagtttcgat ttctaagttt gcttctttca aaattctatc
      121 tggtgtagaa ctttccagta gtggggtcat agtagagtct cttctctggc ccctgcacgt
      181 cttcgggacg gggattgatg accttctcgt ctggttcctt ccagcgttct ctggaagttc
      241 cctggaatcg aggggatgct ttttgcagtc tctggagcat ctcgcgcaaa tattcctgtt
      301 cctgcacctt tcgcctgatg taactttctc tttcgcgcag atcattctgt ccaaatcgaa
      361 gtcccagagc aaaccaggaa tagagcactc ccccgaccag tccgccaaga tgacccatgt
      421 gagaaatgaa agtcaacaca ccaaacatgg ggagcacaat cgaaagaacg ccaatcaaga
      481 tggcagcgtt tctcgcacgc atgggaataa agaagatgag catttgtgaa ttaggataga
      541 acaccgccag tgcaccgagc aaacccagaa caatcccgga tgctccaatg gttgggttgg
      601 gaagatctgc ctgaaataga atgacaaaca aactcgaagc gcagccggaa acaaacatca
      661 accacgcaaa acgaattgaa cccagagtgt tttcaagagc cagcccgaaa ctccagatcg
      721 caagcatgtt aaagccaatg tgtatgatgg gaaagactct accatcatgc aaaaacaaag
      781 cagtcagagg ctgccagaat gcgccgttcc tgaattcagc tggagtcagc ccaaatcgat
      841 ctatcaatgc atccgctgcc tgaggtcctt gcgtgccctg gacataccac cacacgtaga
      901 ggtgaaccag gacattgatc gcgacgaata tcttcgtaac tggctgcttc atctgaattc
      961 cagtttatgg ctttcgctct actccgacag cgtttttttc tcttggttga aggtcagatt
     1021 tcgccctctc cacgatgatg gagtccgggc caggacgaga tttccagcga ttgtgcatcc
     1081 agaagtattg ttccggatac atgcgaatgt atttttccag tatgtccacc catcgcgcgg
     1141 tgaaggccag aatctcagac tcggaacgac cgcatcttcc cagtggaatc atttcaatcc
     1201 tgtacactcc ctggccctga tcaaggccca ctccgaagaa agcttccgag ccagagagtg
     1261 cggcgtagct cgcaggttct ttgtgagtgg atgcaagctg gcccatgaac ggaacaaaaa
     1321 atcctcttga gcctgcgttc tggtctgcaa cgaatcctgc gatttctcct ttcttcaagc
     1381 accgaagcat atccggacta ttcatcatcc gaggcgcgaa gaacttcgca ccgaatcgct
     1441 tgcgaatctt ctccaccatt tcatttgcca gagtgttgac ttgaatggcg gcgtatgcgt
     1501 tgagctgcat tccgcgaagc atgattgcat gagatacgaa ctcccagttt ccgaggtgac
     1561 ccacaaccag aacgattcct ttgccagctc gataggcaga ctcaaaggat ttcagacttt
     1621 ctggttccca ttgaattcta gattccactt cctcttttgt ccagcggccg gaccagtaga
     1681 agtcgtaaga tccttttgcg atggaacaat aggcgtccct catcaactgg tcgatgcgag
     1741 cctcggatgc ttctggaaaa gcgatgcgaa ggttggcccg tgtgcgcttc cttactttgg
     1801 cgaccaggat tctgcccaga aagccaaaca gtctgccaca catgatgcgc cctgccccgg
     1861 accacagacc gaaaaatcga aatactgcgg acgcaaggcc tacttccagc ctccagcgga
     1921 gtttcacggg tcacaaaata aggattgact tgcagccaac cacttatttt tagattcatg
     1981 cctggacgcg atccaagcgg cggttttggc tccggcgtcg acaacgaaga cggactacag
     2041 ctcaagctca cctttgacga agacacaaag actgcatacg gcgaatatcg ttgccctgca
     2101 aagttccgcg gggaaaaaga atccgtacat cccggagtgc tcgcagtcat cctggacgaa
     2161 atcatggcca gaatcaacga agccatgaac ttcgatacag tcacgggaga gctcaccatc
     2221 cgctaccttc agtcggccac cgtggatgag aatctatacc tgcgcggctg gtttgtaaaa
     2281 aagaatcgca gagtggttga aaaccgcgct gaaatcgaaa atgaaatcgg caagattgta
     2341 gcgcgcggca aaggaaagta catcgaaaga gaagactagt ctacggaagt caacttctcg
     2401 gaagtgaact ccaaagaatt cttcttcaac tgcgcaatct cagactcgct cactccccat
     2461 ttcctgacag tttcaaactc tttttctaaa tccgtatcaa agattcctgg atcatctgca
     2521 ccgatcacac agcgaacgtt ggatttcaaa aat
//
LOCUS       DAORFF010000573         2540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_29484_length_2540_cov_3.731187, whole genome shotgun sequence.
ACCESSION   DAORFF010000573 DAORFF010000000
VERSION     DAORFF010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29484_length_2540_cov_3.731187"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PKK76_17585"
     CDS             complement(<1..464)
                     /locus_tag="PKK76_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL70344.1"
                     /translation="MRRKLNWLVFILSAGLAGALWYYTGQLLYPSWRAKDLSVCDART
                     ESYWGKDCGNIRKSGQFQFEEIKIPSTNGYDLPGWKIPSAKNGSGPASGAVFLVHGGG
                     SDRREESRYIQFFVSRRLDVYTFDMSCSGESPCVAAGMTFGHKESRDVQSVYA"
     gene            433..1761
                     /gene="nuoD"
                     /locus_tag="PKK76_17590"
     CDS             433..1761
                     /gene="nuoD"
                     /locus_tag="PKK76_17590"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNL70345.1"
                     /translation="MKTSQFSLRRMVQALASRADLVKPYSGCLRRNIPPNEQRMYSYE
                     TEIIEKEESSILMNVGPSHPATHGTLRLKMKVQGETVIACDQEIGFLHTGFEKLGEYR
                     SYNQFVTITDRMNYISPLNNNIGYALAAEELLGITVPDRCQKIRIILGELSRIADHIF
                     SLGAMTMDLGAFSSILWAFVEREKLYDVFESVTGTRLTTSYTRVGGLAFDLPAGFEQR
                     VTQILVEVEQTIQKMRDSFLENPIFIERTKGVGVITEQDVYDYGLTGPIARASGVNYD
                     VRKFKPYLGYEKFEFKVPIYNEGDSYARYIIRLDEMDESCKIVRQALAELKPGPINYE
                     DQKHVLPDKNSVYHDMESLIHHFKVIMPGGKHGIQPPRKTMYAATEAPNGELGFYIIS
                     DGGANPYKVRVRPPSLFNYQIYSKLMTGANLSDLVTILASFNVIAGELDR"
     gene            1739..>2540
                     /locus_tag="PKK76_17595"
     CDS             1739..>2540
                     /locus_tag="PKK76_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70346.1"
                     /translation="MLANSIDSAALHPLHFVFTGGVLSALFAAGYWISGQKRSRVLAA
                     IFLCLAVVLIHGYLVLSREILLVPRLLYVHVPFLYLLGPLSFLWLRLLLEEDPPHKWL
                     HYIPAALMLVLMTPLYLADPEWKRSLALGFYQGNVPLPARFVFGGGLLSYVIYIVLSV
                     RFLWPSLRSPDFYKDRSVRIAGLILIYATLICLVAFLSLLLGFPVALHWMLMMLAAFP
                     PALYLLGARYPDLFFEFKTALSKGRYEQSRLQGMDLGDLRSRIERLMRE"
BASE COUNT          597 a          654 c          617 g          672 t
ORIGIN      
        1 gcatacacag attgaacgtc tctggattct ttgtgtccga acgtcattcc tgctgccacg
       61 caaggcgatt cacctgagca actcatgtca aacgtataga cgtccagtct gcgcgaaacg
      121 aagaactgaa tatagcgact ctcttcccgc cgatctgatc cgcctccgtg cacgaggaac
      181 accgctccgc tcgccggacc agatccgttt ttcgcagatg gaattttcca tcctgggaga
      241 tcgtatccgt tggtagaagg aatcttgatc tcttcgaact ggaactgccc ggacttgcga
      301 atgtttccac agtctttgcc ccagtaggat tcggttcggg cgtcgcagac agacaggtcc
      361 tttgccctcc aggatggata gagcaactgg ccggtataat accacaaagc cccggcgagg
      421 ccggcggaca ggatgaaaac gagccagttt agcttgcgac gcatggtgca agccctggca
      481 tcccgtgcgg acctggtcaa accttattct ggttgccttc gtcgtaacat tccgccaaat
      541 gagcagcgta tgtactcata cgaaaccgag atcatcgaaa aagaagaaag ctccattctg
      601 atgaacgtgg gcccttcgca tcccgccact cacggcacac tgcggctcaa gatgaaagtg
      661 cagggggaga cagtgatcgc ctgcgatcag gagattggat ttctgcacac cggtttcgag
      721 aagctgggag agtaccgttc ttacaatcag tttgtgacga ttacggatcg catgaactac
      781 atttcgcctc tgaacaataa cataggctat gcgctggcgg cagaagaatt gctcggcatc
      841 actgtgccgg atcgatgcca gaaaattcgc ataatcctcg gggaactttc gcgcatcgcg
      901 gatcatatct ttagcctggg tgcgatgacc atggatcttg gtgcattcag ctccatcctc
      961 tgggctttcg tagaaagaga aaagttatac gatgtgtttg aatctgttac aggcacgcgc
     1021 ctgaccacca gctacaccag agtgggtgga ttggcatttg atcttcccgc agggtttgag
     1081 caacgagtta cacagatcct ggtggaggta gagcaaacca ttcagaagat gcgcgatagc
     1141 tttcttgaga atccgatttt catagagaga actaaaggag tcggagtcat cacagagcag
     1201 gatgtgtacg actatggtct aactggcccg attgcacgcg ccagtggcgt aaactacgat
     1261 gttagaaaat tcaagccata tcttggttat gaaaaattcg aattcaaagt acccatttac
     1321 aacgaaggtg acagttacgc gcgttatatc attcgcctcg acgaaatgga cgaaagttgc
     1381 aagatcgttc gtcaggcact cgcagagcta aaaccaggac ccattaatta cgaagatcag
     1441 aagcatgtcc tgccggacaa gaacagtgtc tatcacgaca tggaatcgct catccatcat
     1501 ttcaaagtga tcatgccggg aggcaaacat ggaattcaac ctcccaggaa aacaatgtat
     1561 gcggccacag aagcgcctaa cggtgaactg ggattctaca tcatttcaga tggcggtgca
     1621 aatccataca aagttcgagt ccgtcctcct tctcttttta actaccagat ctacagcaaa
     1681 ctcatgacag gcgcgaatct ctctgacctg gtcacgatcc ttgcttcttt caacgtaatt
     1741 gctggcgaac tcgatcgata gcgcggcact tcatcctctg cactttgtct tcacaggcgg
     1801 agtgctgagc gctctttttg ctgcaggtta ctggatttcc ggacaaaaaa gaagtcgcgt
     1861 gctcgctgcg atttttcttt gcctcgctgt ggttttgatc catggatacc tggttctcag
     1921 tcgcgagatt cttctggttc cgaggctcct ctacgttcat gttccgtttt tatatttgct
     1981 tggaccgtta tcgttcttgt ggttgcgcct tcttctggaa gaagatccgc cgcacaaatg
     2041 gctccactat atcccggccg cgctcatgct tgttttaatg actccattgt atctggcgga
     2101 tccagaatgg aaacgctctc ttgccctggg attctatcaa ggcaatgtcc ctcttcccgc
     2161 caggtttgta ttcggaggcg gacttctttc ctatgtaatc tatattgttc tttctgttcg
     2221 gtttctatgg ccttctctcc ggtcccctga cttttacaaa gatcgaagcg ttcgcattgc
     2281 aggcctcatt ctgatttacg ctacactcat ctgtctggtt gcgttccttt ctcttctact
     2341 tggctttccg gttgctcttc attggatgct gatgatgctc gcggcttttc ctcccgcgct
     2401 ctatcttctg ggggcccgct atcccgactt gttttttgaa ttcaagacag ccttgagcaa
     2461 aggcagatac gagcagtcga gactccaggg aatggatctc ggagatttac gatctcgcat
     2521 tgaaaggcta atgcgcgaag
//
LOCUS       DAORFF010000574         2538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_29524_length_2538_cov_3.816351, whole genome shotgun sequence.
ACCESSION   DAORFF010000574 DAORFF010000000
VERSION     DAORFF010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29524_length_2538_cov_3.816351"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PKK76_17600"
     CDS             <1..1020
                     /locus_tag="PKK76_17600"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNL70347.1"
                     /translation="MSLGGSVFDSAFSTAITNARTAGVAIVVAAGNDGSNLNSANTYP
                     CEYSQDNVLCVAALDQSYSLATFSNYDTTSVALNNVDVGAPGTNILSSFAGVTTTVND
                     TFNTSGTLNWSTTGSGWGYSACNLGQVVDVIGNPTTWCSLGQYANNANDTIYKTFNVP
                     SGADMVSVAYGLFLDVASGDTFSVHYKSGSGEPFSSGTQLASYTNVSTGGSPVFLGHE
                     LKNCAGQSQCSVGYRLVTNASGTTYGPGILLFSLIGMDVDATTQYKVEDGTSMASPAA
                     AGVVALVLSYNPSYTYLDALNAVLNSGNAQTALNGKSKTGKSVDANNAIRYVAPPTGI
                     SLTAQ"
     gene            1017..1328
                     /locus_tag="PKK76_17605"
     CDS             1017..1328
                     /locus_tag="PKK76_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70348.1"
                     /translation="MRQFLCWAFMSLSIQAAQMGQVIPGEYLVRSEKSIEQLERAFSS
                     TGSVSVSRVEPGLYLLRFEKDPGLDVLVRLSAKEGLGTVQPNFRYSTQPGSGGLHRPV
                     R"
     gene            complement(1270..2007)
                     /locus_tag="PKK76_17610"
     CDS             complement(1270..2007)
                     /locus_tag="PKK76_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNL70349.1"
                     /translation="MFFLDRRRRKKIPVSLLVFGWLFLLLPVYNYFGASQRLHVPMNY
                     PEAILLRMSVIEVILLFAAWPVACGFLLGKKWGWWSFLTYGAALIAHNLTAVLYWRAI
                     GGVSALVQTMVLIAIILYVTRPDISAPFFRTYPRGWRLQKRRPVKTSVLVEGKELQTR
                     DISPRGIYITEALANQSPGAPVNVQFVVHGPVVNGAIVRIDDAGTGIAFRGLNSSQKK
                     LISEWVKQVTEQGDASPRNQAAWNSES"
     gene            complement(2088..>2538)
                     /locus_tag="PKK76_17615"
     CDS             complement(2088..>2538)
                     /locus_tag="PKK76_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70350.1"
                     /translation="EMFRSQLLFMEDLSMTANDLLPGVQMTLLRRYADASREIDFDFT
                     DEAATCLSSQTEISFPLKRRLDLFFLIVELCTNDLKHGKGESLWRISTHEGKLCIVQK
                     NGKRTPEEISAESPASATDRVHRLRGTIKALSGVDEYSIRIEIPLNG"
BASE COUNT          620 a          639 c          634 g          645 t
ORIGIN      
        1 atgagtcttg gtggctctgt ttttgattct gctttttcaa ctgccatcac caatgctcga
       61 acggcaggag ttgctatcgt cgtcgctgca ggaaacgacg gaagcaatct gaattcagcc
      121 aacacctacc cgtgcgaata cagtcaggac aacgtccttt gtgtcgcagc cctggaccag
      181 agctactcgc tggctacgtt ttccaattac gacaccacta gcgttgcctt gaacaacgtg
      241 gatgtaggtg ctccgggcac caatattttg agttctttcg caggagtaac aaccactgtg
      301 aatgatacat tcaacacatc tggaacactc aactggtcta cgactggttc tggatggggt
      361 tacagtgcgt gcaatcttgg ccaggtcgta gatgtgatcg gcaatccaac gacctggtgt
      421 tcgctggggc aatacgcaaa caacgcaaac gacacaatct acaaaacatt caatgtgcct
      481 tctggcgcag atatggtatc cgtcgcatac gggttgtttc tcgatgtggc gagcggggat
      541 acattctctg ttcattacaa aagcggatcc ggcgaaccat tctcctccgg cacacagctg
      601 gcctcctata caaacgtttc aaccggcggc tctcccgttt ttctaggtca tgaattgaag
      661 aactgtgcag ggcagagcca gtgcagcgtt ggttacaggc tggtgacaaa tgcatcaggg
      721 acaacctatg gccccgggat tcttcttttt tctctcatcg gcatggacgt ggatgcgacg
      781 actcagtaca aagtggaaga cggcacttcc atggcttccc ccgccgcagc cggtgttgtg
      841 gccctggttc tcagctacaa tccgtcttac acatacctgg acgctctcaa tgcagttctc
      901 aacagcggaa acgcgcagac agctctcaat ggcaaaagta agacaggcaa atctgtagat
      961 gcgaacaatg caattcgcta cgttgctcca ccgactggaa tcagcctgac tgctcaatga
     1021 gacaatttct ctgctgggca ttcatgtctt tgtctataca ggcagcccag atggggcagg
     1081 tgattccagg tgaatatctg gttcgatctg aaaagtcaat tgagcaactg gagcgcgcgt
     1141 ttagttccac tggatccgta tccgtttcgc gcgtggaacc aggtctctat ctattgcgat
     1201 ttgaaaaaga tccggggctg gatgttctgg tgcgtctttc tgcgaaagaa ggactgggaa
     1261 cagttcaacc taactttcgc tattccacgc agcctggttc cgggggcttg catcgccctg
     1321 ttcggtaacc tgcttcaccc attccgatat aagttttttt tgcgatgagt tgagtcctcg
     1381 aaatgcgatt ccggttccag cgtcatcgat tcgaacaatg gcaccattca caacaggacc
     1441 atggaccaca aactgaacgt tgaccggggc acccggcgac tgatttgcaa gcgcttctgt
     1501 gatgtagatg ccacgagggg aaatatcccg tgtttgcaat tccttacctt ccacaagcac
     1561 tgaagttttc acaggtcgcc ttttttgcag acgccagccg cgaggatacg tgcgaaagaa
     1621 aggagcagaa atatccggcc tggttacgta cagtatgatt gcaatgagga ccatggtttg
     1681 cacgagggca gacactcctc cgatagcgcg ccagtagaga acggcggtca ggttgtgcgc
     1741 gatcaaggct gccccatagg tgaggaaact ccaccatccc cacttttttc ccagaaggaa
     1801 accacaggcc acgggccagg cggcgaacag tagaatgact tcgattacag acatgcggag
     1861 caggattgct tctggataat tcatcggaac gtggagtctc tgagaggccc cgaagtagtt
     1921 atagaccggc agcagtaaga acagccatcc gaacacgagc aatgatacgg gtattttttt
     1981 tctgcgtctt cgatctagaa aaaacatgat gtatggtgtc ttccaatgtc ccggtgtaaa
     2041 tcagaatatg gatgaatttg aaaatgcttt tgattttttc aacggactta gccgttcagc
     2101 gggatctcaa ttcgtatgct gtattcgtcc acaccgctta aggctttgat cgtacctcgc
     2161 aggcgatgca ctcgatctgt tgcgctggcg gggctctcgg cacttatttc ttctggtgtt
     2221 ctttttccat tcttctgaac aatgcacagc ttcccttcat gggtgctgat tctccagaga
     2281 gattctccct tcccgtgttt tagatcattc gtacacagtt caacgatcag aaaaaacaga
     2341 tccagtcttc tcttcaatgg aaaagaaatc tccgtctgtg aagatagaca ggtggctgct
     2401 tcatccgtga agtcaaagtc gatttcgcga gaggcgtccg cataacggcg tagtaaggtc
     2461 atttgcacgc ctggaagaag atcgtttgca gtcatggaca ggtcttccat gaacaaaagc
     2521 tgggaacgaa acatttca
//
LOCUS       DAORFF010000575         2521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_29903_length_2521_cov_3.207218, whole genome shotgun sequence.
ACCESSION   DAORFF010000575 DAORFF010000000
VERSION     DAORFF010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29903_length_2521_cov_3.207218"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1026)
                     /gene="tsaD"
                     /locus_tag="PKK76_17620"
     CDS             complement(28..1026)
                     /gene="tsaD"
                     /locus_tag="PKK76_17620"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNL70351.1"
                     /translation="MFGIGIETSCDETSVAVIEDGLIMRSCAIYSQVADHARFGGVVP
                     EIASRSHLEKINSVFAQAMDDSGVRMEDLSYCAVTTRPGLVGSLMIGGILAKSIHLAF
                     GTPIVTCDHLEAHFYAAALDAGKPLVYPFLGLLLSGGNSAIFIVRGPGDLEQIADTAD
                     DAIGEAFDKTAATLGLSYPGGPAVESCASGYVEKSPSIFTPLLRNEQGLLFSFSGIKT
                     AVTKAKREGHDFAKICHDFQETCFELVTRMLTRAVKQTGIDQVVASGGVIANTILRSR
                     LESAAASGKFDLRYPAKKILCTDNGAMIGALGFFLFQKGKKDVVGFAVSDRRTQNP"
     gene            complement(1030..2394)
                     /locus_tag="PKK76_17625"
     CDS             complement(1030..2394)
                     /locus_tag="PKK76_17625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNL70352.1"
                     /translation="MNLKVAALILCMAIAPAIAQNAPPSRGEKTSQAQIEASHMYQNV
                     LQQVLYLIENYHVDSVSRAKLFEGAVRGMLEATGDPYSRFLNREEQKDFNHSEGGDLV
                     GIGVEITIQGGYPLIVAPMRGGPAEKAGLMTGDRIISIGGQPTRYVGVSKLSELISGE
                     PGSIAELGIQRGGSSFPVRVERGVFQLEYVRTAFLADSRVGYVRLQAFFGEDAGSISK
                     FKKAVQDFARQNVKGIIIDLRNNTGGHVDMARTLSGYFLKKGDVVYRVKSRTDSREFV
                     ADGDTELVPPATKVVILVNRGSASASEILSGCLQDHGRAILVGEQTFGKASVQQVFRP
                     LPDETAVLVTTQRYLTPKNRAIHGIGLKPDVEVPDLMPTPQESVYLGSIEEKKMLEAF
                     LKENPQFDPSLVDKFAKRIAAENMSLRPEIVRLLLKREYGIREPDPDTQSDPQLRRAL
                     ELIQ"
BASE COUNT          601 a          675 c          594 g          651 t
ORIGIN      
        1 gtacccagtt gaggccagcg cggatgttca tggattctga gttcttctgt ctgacactgc
       61 aaatcccaca acatcttttt tgcctttctg aaacagaaag aatccaagcg ctccgatcat
      121 cgctccgttg tccgtgcaga ggattttctt tgccggatat ctcagatcga atttgccact
      181 ggctgccgcc gactcaaggc gcgatcgcag aatcgtgttt gcaatcactc cgcccgaggc
      241 gacgacctgg tcgattccgg tttgcttgac cgctcttgta agcatccgcg ttacaagttc
      301 gaaacaggtc tcctgaaaat cgtgacaaat ctttgcgaaa tcgtgtcctt ctcgttttgc
      361 ttttgtgact gcggtcttga ttcctgaaaa agaaaacagg agtccctgct cgtttcgaag
      421 cagaggagta aaaattgacg gactcttttc gacgtatccg gatgcgcaag attccacggc
      481 cggcccgccc ggataggaaa gacccaaagt ggctgccgtc ttatcaaatg cttcgccgat
      541 cgcatcatca gcggtgtctg caatctgttc cagatcgcct ggccctctca caatgaagat
      601 ggcggaattc ccgccgctca atagaagtcc aagaaacgga tacacaagtg gtttgcccgc
      661 atcgagagcc gcagcataaa aatgcgcctc tagatgatcg cacgttacga tgggcgtgcc
      721 aaaagcaaga tgtatagatt ttgcaagtat tccgccgatc atcaaagatc caacaagtcc
      781 cgggcgagtg gtcacagcgc aataggacag atcttccatg cgaacaccgg aatcatccat
      841 tgcctgcgcg aagactgaat tgattttttc aagatgcgat ctgctggcga tctctggaac
      901 cactccgcca aacctggcat gatccgcgac ctgagagtag atggcgcacg atctcatgat
      961 caggccatct tcaatgactg ctactgacgt ttcgtcgcag gacgtttcaa tcccgatgcc
     1021 gaacatgaac tactgaatca actcaagcgc acgacgaagc tgcggatcgc tttgagtgtc
     1081 tggatccggt tcgcgaattc catactctct ctttaagaga agacgaacga tctcagggcg
     1141 aagagacatg ttttcagcag caattcgttt tgcgaattta tccacaagag aaggatcaaa
     1201 ctgtggattc tcttttaaga aggcttccag cattttcttt tcttcgatcg accccagata
     1261 cacggactcc tggggcgtgg gcatcaagtc aggtacttcc acatctggct tgagaccaat
     1321 tccatgaatg gctctgtttt ttggggtaag atatctctgt gtagtgacaa gaactgcagt
     1381 ttcatccgga agaggacgaa acacctgttg cacggacgcc tttccgaatg tttgctctcc
     1441 gaccaggatg gctcttccat gatcctgaag gcagccggac agaatctcag aggcggatgc
     1501 agaccctcga ttgaccagaa tcacaacttt tgtcgcgggc ggcacgagct ctgtatctcc
     1561 atccgcaaca aactccctgc tgtccgttct gctttttacg cgataaacaa catctccctt
     1621 ctttagaaag tagccagaaa gagtgcgcgc catatccacg tgaccgcccg tgttgttgcg
     1681 aagatctatg atgattcctt ttacattctg ccgggcgaaa tcttgaacgg ctttcttaaa
     1741 tttggaaata gaacccgcat cttctccaaa aaatgcctgc agccttacgt agcccacgcg
     1801 cgaatctgcg agaaatgcgg tgcgcacata ttccagctgg aatactccgc gttccactct
     1861 gacaggaaat gagcttccgc ctctctgaat tccaagctcc gctatggacc ctggctctcc
     1921 gctgatcaat tcagatagtt tagaaacacc aacatatctg gttggttgcc ctccaatgct
     1981 gatgatgcgg tctcctgtca tgagtcctgc tttttcggca ggaccaccgc gcattggtgc
     2041 aacaatcaaa ggatatccac cctgaattgt aatttcaact ccgattccga ccaggtctcc
     2101 accttcagaa tgattgaagt ccttctgctc ttcgcggttt agaaaacgag aataaggatc
     2161 ccctgtggct tcgagcattc ccctgactgc gccttcgaaa agtttggcgc gcgatacaga
     2221 atccacatga tagttttcaa tgagatagag cacctgctgc agcacgtttt gatacatgtg
     2281 cgacgcttcg atttgggctt gagaagtttt ttcccctcgc gatggagggg cgttctgcgc
     2341 gatcgccggt gcgatcgcca tgcaaaggat gagggccgca actttcaaat tcatggctta
     2401 tccccggcaa gagttttgta cagctctgga ttgcgcgtcc ttacaatgtc ttttaccagg
     2461 tcgcatttca ctctcttgct cctgcaggca agttcgaaga tctctttgtc cgtcttctgg
     2521 g
//
LOCUS       DAORFF010000576         2521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_29914_length_2521_cov_2.680049, whole genome shotgun sequence.
ACCESSION   DAORFF010000576 DAORFF010000000
VERSION     DAORFF010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29914_length_2521_cov_2.680049"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PKK76_17630"
     CDS             <1..665
                     /locus_tag="PKK76_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70353.1"
                     /translation="RLSDDALKFAGPQFIHRKMQAMIRYSPVLFALLALLQQPLHSEN
                     SGHSVRNSSFLPAEPFVLNFLYFPSPRNWTWNYAPVDGNHTLVFSLPKNEKLGTSIAI
                     RFREDETGEEFDRFQKVMQDLQKSGAKPPEQLPADILEQTATKKDAFMVYMANGDIEF
                     LTLYYFKNYKQFVAMRLIFSPKPALSSEELSQVREMAFQFIQSVKKIEVVNTMKKSVC
                     EK"
     gene            728..>2521
                     /locus_tag="PKK76_17635"
     CDS             728..>2521
                     /locus_tag="PKK76_17635"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNL70354.1"
                     /translation="MLIKRLLVALSFLILGSSAQAQTLHFIITADTKDPSIGSSVAVD
                     VANLKTMAGQIADATGMTLKLQLLTGSKFTESSFESMLSNLKPGSNDTVFYYNSSHGY
                     RTSRKSSQWPYIYVPDSSKDWDLGAIHDTLAKKGARLTIVMGDQCNSYSDRAMPKDMP
                     RIGKNPLEKGNYKALFLRTKGSIIVTASSPGEYSWGAAQGGEYTIAWREALADAVTED
                     HASWKKLLNNATRVIHAGTPEKQTPLYKTYSLVEGITSTTTSEVPPLASYTLDGGTFK
                     RLSKDDWVLNWNAKQYNMSTYRPGTARYSYLYDKSNDDWYCVDVVDDKLWYWRKTSKE
                     WEIWREIKRAEKPNLLSYTLDGGTFVQNSANQWTLDWSGKRYVMAPYREGTARYTYLY
                     VKDADDWYYVDKTESKLYYWDKDQDDWRMWRTLKKKVADAGSSSYPALACATEEEMQL
                     HKLIMEYRAKNGLAAIPLSKSLTHVARTHVRDLASSKPAGTCNMHSWSIGNQAWTGCC
                     YTPDHAQSRCMWKKPGELTSFKGNGYEIAYGTSGYSATPAGALAGWKSSSGHNNVILN
                     KDKWGSHPWQSVGVGMYGGYAVVWFAEESDTD"
BASE COUNT          741 a          633 c          595 g          552 t
ORIGIN      
        1 accgcctgag tgacgacgca ctcaaatttg ctggaccgca gtttatacat cgtaagatgc
       61 aagccatgat ccgttattcg ccagtccttt ttgctctact tgctctttta cagcaaccac
      121 tccattcaga aaattcaggg cactctgttc gcaactcgtc attcttgccc gcagagccct
      181 ttgttttgaa ttttttgtat ttcccatctc ccaggaattg gacctggaat tatgcaccgg
      241 tagacggaaa tcacactctg gtgtttagcc tgcccaaaaa tgaaaagctg ggaacatcca
      301 ttgccatccg ctttcgcgag gacgaaacgg gagaggaatt tgacaggttc caaaaagtga
      361 tgcaggacct gcaaaaatcc ggcgcaaaac ccccggagca gctgcccgca gacattctgg
      421 aacagactgc cacgaaaaag gatgcattta tggtttatat ggccaatgga gacattgaat
      481 ttctaacatt gtattacttt aaaaattata aacaatttgt tgcaatgaga ctcatctttt
      541 ctccgaaacc tgctctttca tctgaagaac tttcgcaagt ccgcgaaatg gctttccagt
      601 tcattcaatc cgttaagaaa attgaggtgg tcaacacgat gaagaaatcc gtctgcgaaa
      661 aatagaaaat cttgtaaggg ctctaaaaat tgtttgaccc ggcacgaagg atcagcgtca
      721 aaccttcatg ctcatcaaac gactcttagt cgctctatct tttttgatcc tcggatccag
      781 cgcgcaagcc cagactctgc atttcatcat caccgcagac acgaaggatc caagcatcgg
      841 gtcaagtgtg gccgtggacg ttgcgaatct aaaaacaatg gccggtcaga tcgcagatgc
      901 gactggtatg acgctcaaac tgcagctact gactggaagc aagttcactg aatcgagctt
      961 tgaatccatg ctctcaaatc taaagccagg atccaatgac actgtatttt actataactc
     1021 aagccacggg tacagaacct ccaggaaatc ttcgcagtgg ccatatatct atgttccaga
     1081 ctcttccaaa gattgggacc tgggggcgat ccatgacaca ctcgccaaaa aaggcgcacg
     1141 attgacgatt gtaatgggag atcaatgcaa cagttattcg gatcgcgcga tgccaaaaga
     1201 catgcctcga attgggaaaa accctctcga aaaaggaaac tacaaggcac tctttcttag
     1261 aacaaaagga tccatcatcg tcactgcttc cagtcccggc gaatactcct ggggcgcggc
     1321 gcaaggcgga gaatacacga tcgcctggag agaagcgctc gcagatgcgg tgacagaaga
     1381 ccatgcaagc tggaaaaagt tactaaacaa cgcaacccgc gtcattcatg caggaacgcc
     1441 ggaaaaacaa acaccgctgt acaaaactta ttccctggta gaaggaatca catcaacgac
     1501 gaccagtgaa gtgccacctc ttgcatctta cactctagac ggcggcactt tcaaaaggct
     1561 ttcgaaagac gactgggttc tgaactggaa cgcaaagcaa tacaacatgt ccacgtatcg
     1621 cccaggcaca gcgcgctact cctatctata cgacaaatca aacgatgatt ggtattgtgt
     1681 ggacgtagtg gatgataaac tctggtactg gagaaagact tctaaagaat gggaaatctg
     1741 gcgtgaaatc aaaagagctg agaaaccaaa tctactgagc tacactctgg acggcggaac
     1801 ctttgtacag aactctgcga accagtggac tctggactgg agtggcaagc gctacgtaat
     1861 ggctccctat cgcgagggca cggcgcgtta cacatatctg tatgtaaagg atgcagatga
     1921 ttggtactac gtagataaaa cagaaagcaa actctactac tgggataaag atcaagacga
     1981 ctggcgcatg tggagaacac tcaagaagaa agtcgcagac gcagggtcct ccagctaccc
     2041 ggcgctagcc tgcgccaccg aagaagaaat gcaactccac aaactcatca tggaataccg
     2101 tgcgaagaat ggtctagctg ccattccgct ttccaaatcg ctcactcatg tagcgcgcac
     2161 gcatgttcga gaccttgcat cgagcaaacc tgccggcacc tgcaatatgc acagctggtc
     2221 cattggaaac caggcatgga ccggttgctg ctatactccg gatcacgccc agtccagatg
     2281 catgtggaag aaaccaggag agctcacgag cttcaaaggg aatggatacg aaatcgctta
     2341 tggcacgtcc ggctattctg cgacgcccgc gggagcgctg gcaggatgga agagcagctc
     2401 aggccacaac aatgtgatct tgaacaaaga caaatggggc agtcatccct ggcaatcggt
     2461 cggggttgga atgtatggcg gctacgcagt cgtctggttt gcagaagaat ccgacacaga
     2521 c
//
LOCUS       DAORFF010000577         2501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_30377_length_2501_cov_3.309485, whole genome shotgun sequence.
ACCESSION   DAORFF010000577 DAORFF010000000
VERSION     DAORFF010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30377_length_2501_cov_3.309485"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1119)
                     /locus_tag="PKK76_17640"
     CDS             complement(154..1119)
                     /locus_tag="PKK76_17640"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNL70355.1"
                     /translation="MIWAVTGATGFLGRHLVEASLSRGATVRALSRTIVPPSVEHFEG
                     DLLEPDPAGIAAFLKGADVLFHLAGMVSRQKADAEKMMRLHIEGTRAILEGAKKAGVR
                     RVVLASTSGTVGIFEDERTADDNSPYAMAVASEFAYYRSKIYQEKLAMDFAKSSDLEL
                     VCLRPSLLLGPGDHRLSSTGDVKAFLDKKFPVIPAGGISFVDARDTAAAFVEAAVRKI
                     APLPRTYLLTAENWSMARFVREMEEVSGVRAPTKQLNKSILDIGSSIVKLLGEPSFLP
                     DAESMKMASLYWYVDATRAKEELGFNTRPARETLRDTIDFIRAMP"
     gene            complement(1116..1544)
                     /locus_tag="PKK76_17645"
     CDS             complement(1116..1544)
                     /locus_tag="PKK76_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70356.1"
                     /translation="MPSLLHPFIVHVPIAFALFYVPFAWLCIRDRIQWSWFTLYSLCL
                     CASLYGAMASGSVDRAIVEKNRPSISSPLEEHEGSAQKAFFFALAALALSFAGKTRFR
                     EAAKIACGLIALAVLASTIQTARLGGNLVYKHGAAEAHAR"
     gene            1599..2066
                     /locus_tag="PKK76_17650"
     CDS             1599..2066
                     /locus_tag="PKK76_17650"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNL70357.1"
                     /translation="MKNVPGLLILVLLASFCKTAVPAGEKTSDQYIADLSSGDMSLQI
                     TAARKLGERKEEQAIPALVSLLDSNPAPEVAWNVAASLGAIGKPGPATAALQRSANSP
                     SPSVKYASIAALTNLHDKDQKAQNVQAASQMRDSTRDDLLKDLADKAATLMAR"
     gene            2068..>2501
                     /locus_tag="PKK76_17655"
     CDS             2068..>2501
                     /locus_tag="PKK76_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNL70358.1"
                     /translation="MLSRDEITRYSRNILLPEVRKSGQEKLKSSSVLVVGAGGIGSPV
                     LTYLAASGIGRIRCVDSDHLDLTNLQRQVLYRTGGVGSPKIDLAREQIHSLNPNLELD
                     FRMERFSDKNAKDLVTGMDLVIETSDNFPTKFLVSDVCVENR"
BASE COUNT          623 a          636 c          658 g          584 t
ORIGIN      
        1 gtccgtgcaa attggaaatg tcatttcgta tgaggatctg gaagaagata caaaaggact
       61 ttctcccacc aagacatacg acgtgatcgc aaagaaaatc gaaagagcag tccgttcagg
      121 aactcattga tattcctgag cgactgaatc gaatcaaggc atcgcgcgaa tgaaatcgat
      181 cgtgtctcgc agcgtttcgc gcgcggggcg cgtattgaat ccgagctctt ctttcgcgcg
      241 cgtcgcatcc acgtaccagt aaagggaggc catcttcata gattctgcgt ctggcagaaa
      301 ggatggctct ccgagaagtt tcactatgct ggaccctatg tctagaatcg atttattgag
      361 ctgctttgtg ggagcgcgaa ctccggacac ttcttccatt tcgcgcacaa acctggccat
      421 gctccagttc tctgcagtca gcaggtaagt gcgagggaga ggggcgattt ttcgcacagc
      481 cgcttctacg aatgcagccg ccgtgtctct tgcgtccacg aaggagatcc cgcccgccgg
      541 gatgacagga aactttttat caaggaacgc ttttacatct ccggtggaac tgagacgatg
      601 atcccctggg ccaagtagaa gggaaggtcg cagacaaacg agctctagat cgctcgattt
      661 tgcaaagtcc atcgcgagtt tctcttgata gatcttactt ctgtagtacg cgaattcaga
      721 tgcaactgcc atcgcatacg ggctgttatc gtctgcggtt ctctcatctt caaagattcc
      781 aaccgtgcca gatgtggatg cgagcaccac tctgcgcact ccagcttttt ttgctccctc
      841 aagaatggcg cgagtccctt caatatgaag cctcatcatt ttctctgcat ccgccttctg
      901 gcgactgacc attcctgcca ggtgaaatag aacatcagca ccttttaaaa aggcggcaat
      961 ccccgccgga tcaggttcga gaagatctcc ttcaaagtgt tctacggatg gtggaacaat
     1021 ggtccgcgag agtgcgcgca cggttgctcc cctcgaaaga gaagcttcca caagatgtct
     1081 gcctagaaac ccggttgctc cggtaacagc ccagatcatc gcgcgtgtgc ctctgctgct
     1141 ccgtgcttgt ataccaggtt gccgcccagt ctcgcagtct ggatggtgct tgcgagaacg
     1201 gcaagggcga tgagtccgca tgcgatcttc gcggcctccc taaatctggt tttgccagca
     1261 aacgacagtg cgagcgctgc cagagcaaaa aagaacgctt tttgcgctga gccttcgtgt
     1321 tcttcgagcg gagagctgat ggaagggcga ttcttctcta cgatggcgcg atcgacagac
     1381 ccgctggcca tggcgccata gagggaagcg caaaggcaca acgagtagag tgtaaaccag
     1441 ctccactgga tgcggtcacg aatgcaaagc caggcgaaag gaacatagaa cagcgcgaag
     1501 gcaatgggca catgaacgat gaagggatgc aatagagaag gcattggatc gttttttccg
     1561 ttgacatgcc tggaatgcca gatattttac aaggcaagat gaagaatgtt ccgggtctat
     1621 tgattctagt attgcttgca tccttttgca agacagcagt gccggcaggt gaaaaaactt
     1681 cagatcaata catcgcggat ctaagctccg gagatatgtc tcttcaaatc accgcagcgc
     1741 gaaagctcgg tgaacgcaaa gaggagcaag caattcctgc tctagtgtca ttgctggact
     1801 caaatcccgc tccggaagtg gcatggaatg tagctgcctc tctgggtgcg attggcaaac
     1861 caggtccggc caccgcagct ctgcagagat ctgcaaactc tccttcccct tccgtcaaat
     1921 atgcgagcat tgctgcgctg accaatttgc atgacaagga tcagaaagcg caaaatgtgc
     1981 aggctgcttc tcagatgcga gactccacgc gtgatgatct tctcaaagat cttgcagaca
     2041 aagccgcgac tctgatggcc aggtaagttg ctttcgcggg acgaaatcac ccgctattct
     2101 agaaatatac tcctgcccga ggtgcgtaaa tcgggtcagg agaaactcaa atcatcgagt
     2161 gtgctcgtag tcggtgccgg tggaattggg tctcccgtac tcacgtatct ggctgcgtct
     2221 ggaattggca ggattcgctg cgtggactct gatcatctcg atcttacaaa tttacaaaga
     2281 caggtactgt atcgcacggg cggtgtagga agcccgaaga ttgatctggc gcgagaacag
     2341 atccattcat tgaacccaaa tctggaattg gattttagaa tggaacgatt ctcagacaag
     2401 aatgcaaagg acctggtcac cgggatggat cttgtgattg agacctctga taattttccc
     2461 accaagttcc tcgtcagcga cgtgtgcgta gaaaatcgga t
//
LOCUS       DAORFF010000578         2499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_30426_length_2499_cov_4.442308, whole genome shotgun sequence.
ACCESSION   DAORFF010000578 DAORFF010000000
VERSION     DAORFF010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30426_length_2499_cov_4.442308"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PKK76_17660"
     CDS             complement(<1..609)
                     /locus_tag="PKK76_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70359.1"
                     /translation="MLKWVSFVLLSCFSIGLFETHALHSQAQESAQDAANRIAATILS
                     GTNEEQSRAAIREAFAWAGMSTRNSGTVVLGARLPDMPIYLDESEIEILLADAKGRKD
                     GLFRMTLAELGESVNAPESVFPTDPPIPPGTVFRLSILMAVRAARQDPNNVHSFVPLF
                     LDAMAQRSYRGRDITNSRIPGNQFGLSLLEVQLLLSALMRTAR"
     gene            complement(717..1322)
                     /locus_tag="PKK76_17665"
     CDS             complement(717..1322)
                     /locus_tag="PKK76_17665"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNL70360.1"
                     /translation="MPTPEKGILRALVRMSEIRDPYTAGHERRVAHLATAIGTRMRIG
                     EKSLHAVHLAGIVHDIGKIAVPVEILNKPGKLNSHEMGLIRTHPEVGFDILKDSDLPD
                     HVAEIVLQHHERVDGTGYPHGTRNGDIRLESRILAVADVVESMISHRPYRPGLGVEHA
                     LGEIESGIGTRYDGEVVRVCIYLFRDGGFTLPEHGSSSSQQ"
     gene            1570..>2499
                     /locus_tag="PKK76_17670"
     CDS             1570..>2499
                     /locus_tag="PKK76_17670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNL70361.1"
                     /translation="MTTLFETSLNQSPAAVMIVDIHGNIQYVNAAFSRLCGYSSEEVI
                     GKNPRFLKSGDMPKGVYQKLWETLLAGSTWTGEFHNRRKDGTTYWESASVTPVRDDSG
                     SITHFIAFKNEITKHSEDDSREAANRLRMTFNTMSEGVALNEIIFDDHGDMIDYRIID
                     VNAAFYNTADFTGKNVIGNTATALYGMSTESIRAFWQSHKSATTVSHTEMVSPLHQRH
                     FLISVSPFAGNRFVTSFLDITERKRIEAEHTRALELLRTIADNVPAMIAYWTRDLRCA
                     FANRAYEGWFGRKPDEMIGSSLDQILGPELYAKN"
BASE COUNT          674 a          647 c          601 g          577 t
ORIGIN      
        1 tctcgcagtg cgcatcagag cggaaagaag caattgaacc tcaagcagac tcaatccgaa
       61 ctggttaccc ggaatccgcg aatttgtaat atcgcgtccc cggtacgatc gctgggccat
      121 ggcatcgaga aaaagcggaa caaaactatg aacgttgttt ggatcctgac gtgcggctcg
      181 cactgccatt agaatgctca aacgaaacac agtgccagga ggaataggag gatccgttgg
      241 aaaaacagat tctggtgcgt tcacgctctc cccgagttct gcgagagtca tgcgaaacag
      301 tccgtccttt ctgcccttcg catccgcgag taggatttca atttcgcttt catccagata
      361 gatgggcatg tccggcaaac gcgcaccgag cactacagtc ccgctattgc gcgtggacat
      421 cccggcccat gcgaacgctt cgcgaatggc ggcgcgcgat tgttcctcgt ttgtgcctga
      481 gagaatagtc gcggcaattc gatttgctgc atcctgggcg ctctcctggg cctgcgaatg
      541 tagagcatgc gtttcgaaca acccaataga aaaacaggac agaagaacaa aggacaccca
      601 cttcaacatg agtgaatgtt cttgttcttg cgaatcgaaa atcaagtgga aacgaatcaa
      661 gagctcagac tgttcagtat gatctttgtc attttacagg ctgaatcatc gcagtgtcat
      721 tgctgcgatg aagaggatcc gtgttctggc agagtgaatc cgccatcgcg aaatagataa
      781 atgcatacgc gcaccacttc gccgtcatat cgagtgccga ttcctgattc aatctcaccg
      841 agggcatgtt ctaccccaag ccccggtctg taaggtctat gcgaaatcat ggactccaca
      901 acgtccgcga cggcaaggat tctgctttcc agacggatgt ctccgtttct ggtcccgtgc
      961 ggatatccgg tgccatccac tcgttcgtga tgctgcaata caatttctgc cacatggtca
     1021 ggaagatctg aatctttgag aatatcaaaa ccaacttctg gatgcgtgcg aatcagcccc
     1081 atttcatgcg aattgagctt gcccggtttg tttaagattt caacaggaac cgcaatcttc
     1141 ccaatgtcgt ggacgatgcc agccagatga actgcgtgca aagacttctc accaatccgc
     1201 atccgtgttc caatagctgt ggcaagatgt gccacgcgac gctcatgacc cgctgtatac
     1261 ggatcacgta tctcactcat tcgcacgagt gcgcgaagaa tccctttttc cggcgtcggc
     1321 acccggcgaa tatgtcgcac aagggttcct tgtcgatgga aatactgtcc agggttgatg
     1381 aatcattttt gcagggcgaa aaaaacaata ctttgagatc agttctcata agatcttcat
     1441 agagtggaat gcggcgcgcg atttgaagta gacaacttcg acgagaacat tcagaaagaa
     1501 gaagaaacgt gcggatcgct cgtccacgag gcaactcgat tgtgacaatc tagctgccaa
     1561 cctatgaccg tgactacact cttcgaaact tcgctcaacc agagccctgc tgcggtcatg
     1621 atcgtggata tccacggaaa tattcagtat gtaaatgcag ccttcagtcg cctgtgcggc
     1681 tattcaagcg aggaagtcat cggaaagaac ccacgattct tgaaatctgg ggatatgcca
     1741 aaaggagtat atcaaaaact ctgggagaca cttctcgcag ggtccacgtg gaccggagaa
     1801 ttccacaatc gaagaaaaga cggaaccact tactgggagt ctgcgtctgt cacaccggtt
     1861 cgcgatgaca gcggaagcat cactcatttc atcgcattca agaacgagat caccaaacac
     1921 agcgaagacg attcgcgcga agccgcaaat cgattgcgca tgaccttcaa cacaatgtcg
     1981 gaaggagttg ccctgaacga aatcatcttc gatgaccacg gagatatgat agactatcgc
     2041 atcatagatg tgaacgctgc tttctacaat actgctgact tcaccggcaa aaatgtaatt
     2101 ggcaatactg ccacagcatt gtacggaatg tccactgaat ccatccgagc cttctggcaa
     2161 agtcacaaat cggcaacgac agtttcacac acagagatgg tgagccccct ccatcaaaga
     2221 cacttcctga tttctgtttc tccgtttgct ggaaatcgtt ttgttacctc tttcctggac
     2281 attactgaaa gaaaacgaat tgaagcagaa catacacgcg cactcgagct actgagaacc
     2341 attgctgaca atgttccagc tatgattgcc tactggaccc gggatctcag gtgtgccttc
     2401 gcaaataggg cttacgaagg ctggtttggc agaaaacccg acgaaatgat cggctcgtcc
     2461 ctggaccaga tcctgggccc ggaattgtat gcgaaaaac
//
LOCUS       DAORFF010000579         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_30479_length_2497_cov_3.006552, whole genome shotgun sequence.
ACCESSION   DAORFF010000579 DAORFF010000000
VERSION     DAORFF010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30479_length_2497_cov_3.006552"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PKK76_17675"
     CDS             complement(<1..804)
                     /locus_tag="PKK76_17675"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNL70362.1"
                     /translation="MSYIAAAKNGIDRLVDNIRQVIVVQPATLETILASKIAGGHVIL
                     ADAHGVGKTSLARALSGSIDWGEGQTKDGVKIEPFSRIQCTVDLLPQDILGFNRFDMQ
                     SNRTTFHSGPIFANFILCDEINLLTPKTQGSFLQVMEEQRITIEGSTYHLPDPFFIIA
                     TMNLKGSHLFPLPAPQLDRFMVQISLGYPSEQDEIQILTKHGKDDSWKDFKPVIDPNE
                     LLAWQKLVDQVSLHADVASYIVRIIRQTRKHPGVITGASPRSGIKLSRLV"
     gene            complement(812..1015)
                     /gene="rpmE"
                     /locus_tag="PKK76_17680"
     CDS             complement(812..1015)
                     /gene="rpmE"
                     /locus_tag="PKK76_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNL70363.1"
                     /translation="MKPNIHPEYKEAAVKCASCGTVYNMKSTRGNMTVDICSACHPFF
                     TGTERMIDAAGRVDRFKKKYKLK"
     gene            complement(1039..2403)
                     /gene="rho"
                     /locus_tag="PKK76_17685"
     CDS             complement(1039..2403)
                     /gene="rho"
                     /locus_tag="PKK76_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNL70364.1"
                     /translation="MDARSEDPGMTDFDPAAEQLEHENNMRARTGNEPPPLDITELKR
                     KPIEDLTVLAEKLGVENTTGLKKQNLIFGIIQKQAEQNGSILASGTLEKMPEGYGFLR
                     SADYSYLPGPDDIYVSPSQIRLFGLRTGDTVNGYIRPPKESERFFALLRVDTVNGTTT
                     DAAHRRQLFDNLTPLYPDSRLNMEWNPSLLDTRIIDLLTPIGKGQRGLIVAPPRTGKT
                     ILMQNIANAITHNHPEVILMVLLIDERPEEVTDMARSVKGEVVSSTFDEPASRHVQVA
                     EMVIEKAKRQVEHGRDVVILLDSITRLARAYNQVVPTSGKILSGGVDSNALHKPKRFF
                     GAARNIENGGSLTILATALVETGSKMDEVIFEEFKGTGNMEIILDRRLADKRIFPAMD
                     INRSGTRKEELLLAKDVLNRVFILRKVLSPMSSTEAMELLLEKIRVSKTNEDFLGSMN
                     VPTA"
BASE COUNT          555 a          607 c          647 g          688 t
ORIGIN      
        1 cacaagacga ctgagcttga tgccagaacg cggacttgcg cctgtaatta cgcccggatg
       61 ttttctggtc tggcggatga ttcgaacaat ataagacgcg acgtctgcat gaagggaaac
      121 ttgatccaca agcttctgcc acgccaggag ttcgtttgga tcaatgacag gcttgaaatc
      181 tttccaggaa tcgtctttgc cgtgtttggt gagaatttgg atctcatcct gttcggaagg
      241 atagcccaga gaaatctgaa ccataaaacg gtccagctgc ggtgcgggaa gcgggaacaa
      301 gtgacttccc ttcaggttca tggtagcaat gataaaaaat ggatccggaa gatgatacgt
      361 gcttccttca attgtaatgc gctgctcttc catcacttga agaaagcttc cctgagtttt
      421 gggagtcagt agattgattt catcgcagag aatgaagttc gcgaaaattg gaccgctgtg
      481 aaacgtagtg cgattggact gcatgtcaaa tcggttgaag ccaagaatgt cttgtggaag
      541 gagatccaca gtgcactgga tgcggctgaa tggttcaatc ttcactccgt cttttgtctg
      601 cccttctccc cagtctatgg aaccagaaag agcgcgcgca agtgaagtct tacctacacc
      661 gtgcgcgtct gcgagaatga catgaccccc ggcgatcttg gatgcaagaa tcgtctcgag
      721 ggttgccggt tgtacgacga tgacctggcg gatattgtcg accagccggt cgatgccatt
      781 cttggctgca gcaatgtacg acatgctgcg cttacttcag cttgtatttc tttttgaaac
      841 gatccacgcg gcccgccgca tcgatcattc tttctgtgcc ggtgaagaat ggatgacatg
      901 cggagcaaat gtccacagtc atatttcctc ttgtggactt catgttgtat acagttccgc
      961 aagacgcgca cttcacagca gcttctttgt attcaggatg aatattcggt ttcataactc
     1021 tcctcagtag acgctctttc aagctgtagg cacgttcatg ctgccaagaa aatcttcgtt
     1081 tgttttgctg accctgattt tttccagcaa aagctccatg gcttccgtgc tcgacatggg
     1141 actcaggact tttctgagga tgaagacgcg gttgagaacg tccttggcca gcagaagctc
     1201 ctcttttcga gttccagagc gatttatatc cattgccggg aaaatccttt tgtcggcaag
     1261 ccttctatcc aggatgattt ccatatttcc agtgcctttg aattcttcaa agatcacctc
     1321 gtccatctta gacccggttt caacgagtgc ggttgcaaga atagtcaaag aaccgccatt
     1381 ttctatgttt ctggctgctc cgaagaagcg ctttggcttg tgaagagcgt tggaatcgac
     1441 accgccggat agaatcttcc cggaagtagg aaccacctgg ttgtaggcgc gggccagcct
     1501 ggtgatcgaa tcaagcagaa tgaccacatc gcggccatgc tctacctggc gctttgcctt
     1561 ctcaatcacc atctccgcta cctgaacgtg gcgggatgca ggttcgtcga aagtagaact
     1621 gaccacttcc ccttttacgc tgcgtgccat gtctgttact tcttccggac gttcgtcaat
     1681 cagaagaacc atgaggatga cttctggatg attgtgagtg atcgcgtttg cgatattttg
     1741 catcaagatc gtcttacctg tacgcggagg agcaacgatg agacctctct gcccttttcc
     1801 aatcggagtg agcaggtcga tgatgcgagt gtcgagcaaa gacggattcc actccatgtt
     1861 caaacgagag tctggataaa gaggagtcag gttgtcgaaa agctgtctgc gatgagccgc
     1921 gtccgtcgtg gtgccgttga ctgtgtccac gcgaaggagt gcgaagaatc tttccgattc
     1981 ttttggaggg cgaatgtagc cgtttacagt gtctcctgtg cgaagaccga acagacggat
     2041 ctgcgaaggc gaaacgtaaa tgtcgtccgg gcctggtaag tagctgtagt cagcgctgcg
     2101 aagaaagccg tagccttctg gcatcttctc aagagttccg gaggcaagga tcgatccgtt
     2161 ttgctccgct tgcttctgaa tgattccgaa aatcagattt tgtttcttga gccccgtcgt
     2221 gttttcaacg cctagcttct ctgcgagaac agtaagatct tcgattggtt tgcgttttag
     2281 ttctgtgatg tcgagtggag gcggctcatt tcctgtgcga gcacgcatgt tgttttcgtg
     2341 ttcgagttgt tctgctgctg gatcaaagtc cgtcattccc gggtcttcag atcgcgcatc
     2401 catgcgatcg taaccgccgc ccgggcctct tctgtggccg cctggtcttc tttctctagt
     2461 tcttcctctg ggcatgtgaa atatcctgaa ttgttta
//
LOCUS       DAORFF010000580         2493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_30574_length_2493_cov_3.139459, whole genome shotgun sequence.
ACCESSION   DAORFF010000580 DAORFF010000000
VERSION     DAORFF010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30574_length_2493_cov_3.139459"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PKK76_17690"
     CDS             <1..782
                     /locus_tag="PKK76_17690"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNL70365.1"
                     /translation="SQEDRASGQVSLFGEAMATEDFIPKGEGVSEFEESILLRQEREM
                     LGCYLSGHPLSRFDRVLSRAGISTIENLQRMKANDRVELAGVVGDISKRITKTGKEMW
                     NIRIEDKTGSIEAVLFSSTIEESRNFLQKDTPLIFKGRVEKDDAKPMPRLRVESVKEL
                     TDEAVEEKLEKSLHLQIQTLTSVDGEQVLKQIQTILKCHKGNLNVYFHLPSQQTPGAQ
                     TVIRAHNSYSVAMNRAMIDQLKQLACVRSVYVSIGNQVRPV"
     gene            complement(783..1367)
                     /locus_tag="PKK76_17695"
     CDS             complement(783..1367)
                     /locus_tag="PKK76_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70366.1"
                     /translation="MFDEAGLTPQEILEIEEAFVRQSLVRRGDEEFQALLSEHLMQGE
                     LLPVPQTLLDISGRVLTNSAPEFQIRLHPENVITSVVSAVRGFFSEPLALDPVRSVGL
                     ERRPALDIGKAGSVIRYRIIRESEAEVLLAVRVDAPGALRANLRRDGRIVASQTIAIG
                     ESSLAFEHLGAGNYTLEITGSRAESVSFHINSEV"
     gene            complement(1419..2297)
                     /locus_tag="PKK76_17700"
     CDS             complement(1419..2297)
                     /locus_tag="PKK76_17700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNL70367.1"
                     /translation="MDTPIPSGTEQEAILSLVRRCAGGEDAALREFFETYSRDIYNFP
                     MRVFHLDEDGASDFYLYALEILKNGNRFKSFSGRSTFRTWFYSVLRNLVIDWLRITRF
                     QRKPSQPLLEAADQPVPEEEPLVQDWLSGLPLETRCLVKLVFVYYLEMDAEELAHVCD
                     RSGLNKVKLAERIASIKAGLAEKEIENLTRTDKITSLYVGILDLRSRRARVIEEDTQH
                     VRRHEVERIDHAIAKKTQQREKLLARKEKGHFVVRLPYREAAELLAMPEGSVSVAVMR
                     AFEKLREMQKKLTEGK"
BASE COUNT          624 a          679 c          599 g          591 t
ORIGIN      
        1 agagccagga agacagagct tccggtcagg tgtctctctt cggagaagca atggccacag
       61 aagatttcat tcctaaaggg gaaggagtca gcgaattcga agaatccatt cttctgcgtc
      121 aggaacgcga aatgcttggc tgctatctca gcggccaccc tctctctaga tttgaccgcg
      181 tactttcgcg cgccggaatt tcaacgatcg aaaatctaca gcgcatgaaa gcaaacgaca
      241 gagtagaact cgccggagta gtgggagata tttccaaaag aatcacaaag accggcaaag
      301 aaatgtggaa cattcgaatt gaggacaaaa caggatccat cgaagcagtc ttgttctcat
      361 ccacaatcga agaatcacgc aatttcttac agaaagacac tccactgata ttcaaggggc
      421 gcgtagaaaa agacgacgca aaaccaatgc ctcgccttcg cgtagaatcc gtgaaggaac
      481 tcacagatga agcagtagaa gaaaaactcg agaaatcact gcacctgcag attcagactc
      541 tcacatccgt agatggggaa caggttctca aacaaatcca gaccatcctg aagtgtcaca
      601 agggaaatct aaacgtctat tttcatttgc catcgcaaca gacgcctgga gcacagacag
      661 taattcgcgc gcacaacagc tacagtgttg ccatgaatcg cgccatgatc gatcaactca
      721 agcagctggc ctgcgttcgc tctgtttatg tttccattgg aaaccaggtt cgacctgttt
      781 gatcagactt cgctgttgat gtgaaatgaa acggattcgg ctcggctgcc tgtgatttca
      841 agcgtgtagt ttcccgctcc cagatgctcg aaggccagag aagattcgcc aatggctatg
      901 gtctgcgaag caacaattct tccatctctt ctcagattcg ctctcaatgc gcccggagca
      961 tccactcgaa cagcaagcag cacctctgct tccgattcgc gaatgattct ataacgaatg
     1021 acactccctg ctttgccaat gtccagagcc ggacgccttt ccaggccaac ggatctgact
     1081 ggatcgagag ccagaggctc ggagaaaaac ccgcggactg cggatactac agaagtgatt
     1141 acattctcag gatgaaggcg tatttgaaac tcgggggcgc tgtttgtgag aacacggcct
     1201 gaaatatcga gtagagtttg cggcacgggc agcaattctc cctgcatgag atgctcagaa
     1261 aggagcgcct gaaactcctc gtctccacgt ctaaccaggg actggcgaac gaacgcttct
     1321 tcaatttcta ggatttcctg gggcgttaag cccgcttcat cgaacattac ggttgcccct
     1381 gccaagctac ataatctttg acggtgaatt ccatattcct attttccttc cgtcagtttt
     1441 ttttgcattt cgcgtagttt ttcgaatgcc cgcataacgg ccacgctcac gctcccttcc
     1501 ggcatggcca acaactctgc ggcctccctg tagggaagac gcactacaaa gtggcccttc
     1561 tctttgcgcg cgagtagctt ctctctctgc tgggtcttct ttgctatggc atgatcgatt
     1621 cgctctactt catgcctgcg cacatgctga gtatcctctt cgatcacccg ggcccggcgg
     1681 ctcctcagat cgagaatgcc tacgtagagt gaggtgatct tatcggttcg ggtcaggttt
     1741 tcgatttcct tctctgcgag gcccgccttg atcgaagcaa tgcgctccgc cagtttcact
     1801 ttgttgagac cggaacggtc gcatacatgg gccagctctt ctgcgtccat ttccagatag
     1861 tacacaaata caagttttac gaggcacctg gtctccagag gcaatccgct cagccagtcc
     1921 tgaaccagcg gttcctcctc cgggactggt tgatccgccg cctcgagcaa aggctgagaa
     1981 ggcttgcgct gaaacctggt gatgcgtagc cagtcaatga ccagattgcg aaggactgaa
     2041 tagaaccagg tcctgaaagt ggatcttcca gaaaatgact tgaacctgtt tccgttcttc
     2101 aagatttcca gggcgtatag atagaaatcg gaagccccgt cttcgtccag atggaacacc
     2161 cgcattggga aattgtagat atctctggaa taggtttcga agaactcacg cagagccgcg
     2221 tcctcaccgc ctgcgcatct gcggaccaga gaaagaattg cctcctgctc tgttccgctc
     2281 ggaatgggcg tgtccatcaa acccacgtca caacaaggcg atgaaatctc gactcctatt
     2341 ttttcttttt gcaatttccg cagccggcgc tttcgcacag gaagttaagt tctcctggcc
     2401 catggaacgc gggtttccag aactccgcgg cctcacctcc accttcggcg aatcacgcgg
     2461 agatcacttt cacaacggag tggatatttc cag
//
LOCUS       DAORFF010000581         2472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_31007_length_2472_cov_3.638808, whole genome shotgun sequence.
ACCESSION   DAORFF010000581 DAORFF010000000
VERSION     DAORFF010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31007_length_2472_cov_3.638808"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1161
                     /gene="leuB"
                     /locus_tag="PKK76_17705"
     CDS             67..1161
                     /gene="leuB"
                     /locus_tag="PKK76_17705"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNL70368.1"
                     /translation="MSHRIAVLAGDGIGPEVMPSAIEILSRALGSEFSNFTFEEAPVG
                     GAAIDQTGEALPASTLKLCESSDAILFGSVGGPKWEHLPPEKQPERASLLPLRKHFKL
                     FANLRPVALYPELEASSPLKAERTVGTDLLVIRELTGDVYFGQPKGREGSGPDEKGFD
                     TMVYHRYEIERIAKVAFESAVGRKRKVTSIDKANVLTSMVLWREVVTQFGKKFNEESK
                     VQVELDHMYVDNAAMQLILKPSRFDVVLCGNMFGDILSDEASVLGGSLGMLASASLSE
                     KQGFGLYEPAGGTAPDIAGKGIANPIAQILSGALLLRYSFGKGDAASKIEAAVKSAIS
                     AGARTIDLAGPGETSISTKEMTQEIARRIS"
     gene            1158..1565
                     /locus_tag="PKK76_17710"
     CDS             1158..1565
                     /locus_tag="PKK76_17710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL70369.1"
                     /translation="MKPDGVAPNGKAYRIALCDDKKFDVSDWKLFLGSRGFYVQSFEN
                     ALELVAAVRKLPGDFDLIVLDLVMPVLDGYAAFFEMKASGRMPKVMFVSVENTPTVVR
                     SLIEAGAADYLARPFTKDVLLERIKKVLARPST"
     gene            complement(1571..2044)
                     /locus_tag="PKK76_17715"
     CDS             complement(1571..2044)
                     /locus_tag="PKK76_17715"
                     /inference="COORDINATES: protein motif:HMM:NF016286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HNL70370.1"
                     /translation="MQDSNEELVAYNRKFKKAVLDVMFSMSDTFYIHCMPNPLLHIGG
                     RGMTDREQREGIVLVFGPYSTRNLNWDEGSILCEMQFNRWEKVRIPFECVSRIFDKSG
                     QVNMQWATLVSSESHRDEPQTAPEGTPVPPEGETPAPRKKDKSRVIEVDFTRKRK"
     gene            complement(2046..>2472)
                     /locus_tag="PKK76_17720"
     CDS             complement(2046..>2472)
                     /locus_tag="PKK76_17720"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNL70371.1"
                     /translation="LMVRTADCLPLFFEGRGEETWVGVIHAGWRGLAAGIIRSTLSRV
                     IELGKIATLKIAPGPCISGKNYEVGPEVAERFRVKESKGAKFHVDLIENAKEQWNFPG
                     VQIELLEDFRGCTVDNNDLYYSHRKGDMGRNLNLIHIED"
BASE COUNT          564 a          630 c          641 g          637 t
ORIGIN      
        1 tccattcgta aagacacgca ctggaaagct cacttgacag tgcgggcgtt ttcgcccgaa
       61 tgatccatgt ctcatcgtat cgctgttctc gcaggagacg gaattggccc ggaggtcatg
      121 ccttctgcga tcgaaatttt gtccagagct ctgggttctg aattttccaa ttttacattt
      181 gaagaagctc ccgtaggcgg agcggccatt gatcaaacgg gagaagccct tccggcttcc
      241 accctcaagt tgtgtgagtc ttctgatgca atcctgttcg ggtctgtagg cggtccaaaa
      301 tgggaacacc ttcctccaga aaaacagcca gaaagagctt ctctcctgcc acttcgtaaa
      361 cattttaaac tatttgcgaa tctaagacca gtcgcactgt atccagagtt ggaagcatct
      421 tctcctctca aagcagaacg cactgttgga acggatttgc ttgtgattcg cgagcttacc
      481 ggggatgttt actttggcca gccaaaggga agagaaggca gcggcccgga tgagaagggc
      541 tttgatacaa tggtgtatca tcgctatgaa attgaaagaa tcgcaaaggt tgcttttgaa
      601 tccgcagtcg gtcgcaagcg caaagttaca agcattgata aggcaaacgt tctgacctct
      661 atggtgctgt ggcgtgaagt ggtcactcag tttggaaaga aattcaacga agaaagcaag
      721 gtgcaggtgg agctcgatca catgtacgtg gacaacgccg ccatgcagct cattctcaaa
      781 ccttcgcgtt ttgacgtagt gctctgcggc aatatgttcg gagatatttt gagcgatgag
      841 gccagtgtgc tgggcggttc tcttggaatg ctggccagcg cttccctttc tgaaaagcag
      901 ggattcggtt tgtatgagcc ggccggcggc accgctcctg acatcgctgg caaaggaatc
      961 gcaaatccaa tcgcacaaat tctatccggc gctctcttac tccggtattc attcggaaaa
     1021 ggggacgccg cctcaaaaat tgaggcggcc gtgaagtctg ccatcagtgc aggagccaga
     1081 actattgatc tggcgggtcc aggagaaaca tccatcagca caaaagaaat gacccaggaa
     1141 attgcgcgca ggatctcatg aaaccggacg gagtggcgcc taacggcaag gcatatcgca
     1201 tcgctctttg cgatgacaag aagtttgatg tctcagactg gaaattgttt ctgggttcgc
     1261 gcggctttta cgttcaatcc tttgagaatg ctttagagct ggtggctgcg gttcgcaagc
     1321 ttcccggtga ttttgatttg atcgttctgg atctggtgat gcctgtgctg gacggatacg
     1381 ccgccttctt tgagatgaag gcatctggcc gcatgccaaa ggtgatgttc gtatccgtcg
     1441 aaaacacgcc gacagtagtg cgcagtctga ttgaagcggg cgcggctgac taccttgcca
     1501 ggccttttac caaagatgtt cttctagaga gaatcaagaa ggtcctggct cgcccttcta
     1561 cctgactggc ttactttctt tttcttgtga agtccacttc gatgacgcgc gatttgtctt
     1621 tctttcgcgg tgcgggagtt tccccttccg gtggaactgg agttccctcc ggtgccgttt
     1681 gcggttcatc gcggtgagac tcactggata caagtgtagc ccattgcatg ttgacctggc
     1741 cactcttgtc aaagatgcgc gaaacgcatt cgaacgggat gcgcactttc tcccagcgat
     1801 tgaattgcat ttcgcataga atggagcctt catcccagtt caggtttcgc gtgctgtaag
     1861 gtccaaacac aagcacgatg ccttcgcgct gctcgcgatc tgtcatgccg cgcccaccaa
     1921 tgtgcaggag agggttgggc atgcaatgga tgtagaacgt gtcactcatg gagaacatga
     1981 catcgagtac agctttcttg aactttctat tgtacgcgac cagctcttcg ttcgaatcct
     2041 gcattttagt cctcgatatg aatcagattg agattccggc ccatatcccc ttttctgtgg
     2101 gagtagtaga gatcgttatt gtccaccgta caaccccgga aatcttctag caattcaatt
     2161 tgcacgcccg gaaaattcca ttgctctttt gcgttttcga tcagatctac atggaatttg
     2221 gcgcctttgc tctctttgac gcgaaatctc tcagccactt ccgggcccac ttcgtaattc
     2281 ttcccgctga tgcacgggcc aggggcaatc ttcagagtcg cgatcttgcc aagctcaatg
     2341 acgcgcgaaa gcgtacttcg aatgattcct gcagccaggc ctctccagcc cgcgtggatg
     2401 acacccaccc aggtttcctc acctctgccc tcgaagaaaa ggggaaggca gtctgctgtg
     2461 cgaaccataa gc
//
LOCUS       DAORFF010000582         2469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_31073_length_2469_cov_2.792875, whole genome shotgun sequence.
ACCESSION   DAORFF010000582 DAORFF010000000
VERSION     DAORFF010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31073_length_2469_cov_2.792875"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PKK76_17725"
     CDS             <1..938
                     /locus_tag="PKK76_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNL70372.1"
                     /translation="VFKENKEFVLHDQHGDPVSALFNPLWGGHTYAFDVTHPRFKEWI
                     SETIRTIVFDWGYPYLKLDFLYSACYRGVYHDPRKGPAVRLRESLDLIRKVAGKKTFL
                     LGCGCPIMPGIGPFDAMRIGRDVNSIWDDNLLATIIKDRNYPTAEGCLQNAITRSFMH
                     RKFFVNDPDCILVRDTNTKLSLDQVHLMTAVMSLTGGMILLSDDLETLSPDRMEIFRM
                     ALKIHKECAKHTALPLGLMEDHFPRGLYNKAGYLGIWNPTSRAERIQILLPEGLGPKL
                     TRSTNLWDNRRIPWRIEDDTLEMSLAPFESVLTKV"
     gene            1028..2086
                     /gene="fliN"
                     /locus_tag="PKK76_17730"
     CDS             1028..2086
                     /gene="fliN"
                     /locus_tag="PKK76_17730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNL70373.1"
                     /translation="MGDGSLTQEEIDLLLQGADDISAAPVTAGAQGGGNQGVADLSPL
                     ERESVADAVHGALQAAVQSLGLILSRSIKLGSPYVDARPFDDVQKEFSSGAVLFSQNL
                     SGGIQGPIGLMLPTGDGARISGLVMGNEGAPAASSLDSAQVATLKDALGPMMFSIATQ
                     LSIKISQAITPMPVEVKTSGMIPFAQDSAILRIQIPFTVEGAVDTRLTLIMPMAMARD
                     LYRKLKSGPTSSMETGMQDTRSSGQGTLGVKEVSFPSLSSSGAGPLQPNMNLLLDVQM
                     TLTVELGRTKMFIKDILGLGEGSIIELDKLAGEPVDLYVNDRLIARGEVVVIDENFGV
                     RVTDIVSRAERLKAQKQG"
     gene            2128..>2469
                     /locus_tag="PKK76_17735"
     CDS             2128..>2469
                     /locus_tag="PKK76_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70374.1"
                     /translation="MRHKIRIGALALLVLTVPIFGEKPPKTQKPETPSVDNRVKEIDK
                     SWMATIHGNPSDPKPDAAKTDDSSPNVTTTQDSARDNADQSTAAKPGSAEKPDENPTR
                     LRNPGSDPKSLF"
BASE COUNT          637 a          678 c          619 g          535 t
ORIGIN      
        1 atgtctttaa agaaaataaa gaattcgttt tgcacgatca gcacggagac cctgtcagcg
       61 ctttgttcaa tccactctgg ggagggcata cgtacgcctt cgacgtcact catcctcgct
      121 tcaaagaatg gatttctgag accattcgca ccatcgtttt tgactggggc tatccctacc
      181 tcaagctgga ctttctgtat tctgcctgtt atcgcggagt gtaccacgat ccacgcaaag
      241 gcccggctgt caggctcaga gagtcgctgg acttgattcg caaagtcgca ggaaagaaaa
      301 cattccttct tggatgcggt tgccccatca tgcctggcat tggtccattt gacgcgatga
      361 ggattggccg cgatgtcaat tcaatctggg atgacaacct ccttgccaca atcataaagg
      421 atcgtaacta tccaaccgcg gaaggatgtc tgcaaaatgc gattacgcga tccttcatgc
      481 acaggaaatt ctttgtaaac gatccagact gcatcctcgt gcgcgatacg aatacgaagt
      541 tgagtctcga ccaggtgcat ctgatgactg ctgtgatgtc tcttacaggc gggatgatct
      601 tactgagcga tgatctcgaa actctgagcc cggatagaat ggaaatcttt cgcatggcgc
      661 tcaaaattca caaagaatgc gcgaaacaca cggcccttcc cctgggtttg atggaggacc
      721 acttccccag aggactctac aataaggcag gctacctggg aatctggaac ccgaccagcc
      781 gcgcggaaag aatccagatc ctcttgcctg aaggtctggg acccaaattg acccggtcta
      841 ccaatctctg ggacaacaga cgcatcccct ggaggattga agatgacacc ctggaaatga
      901 gcctcgctcc ctttgagagc gtgcttacaa aggtttagaa aaagtagtgt acaggcctgc
      961 gcacgatgcc ctgctcggga aaaagagtgc tggatttgtc aggattcttc gacattgagt
     1021 acaggctatg ggtgacggtt cactaacaca ggaagaaata gatcttctac tccagggcgc
     1081 agacgacatc agtgccgctc cagtcaccgc aggcgcgcaa ggcggcggaa accagggcgt
     1141 tgcagatctg tccccgctgg aacgcgaatc ggtcgcagac gcggttcatg gcgcacttca
     1201 ggccgccgtt caaagccttg gcctcatcct ttctagatcc atcaagctgg gcagtcctta
     1261 cgtggatgcg cgcccattcg acgacgttca aaaagaattc tcaagcggtg cggttctctt
     1321 ttcacagaat ttgtcaggcg gcattcaggg tcctatcggc ttgatgctcc ccacgggaga
     1381 cggcgctcga atcagcggcc tcgtgatggg aaacgaaggt gctccagccg catctagcct
     1441 ggacagcgca caggtggcta ctctcaaaga cgctcttggc cccatgatgt tctcgattgc
     1501 aacacagctt tcgatcaaga tcagtcaggc aatcacaccc atgcccgtag aagtaaaaac
     1561 ctccggaatg atccctttcg cgcaggattc tgccatcctt cgcatccaga ttcctttcac
     1621 agtggaagga gctgtggata ctcgcctgac gttgattatg cccatggcaa tggcgcgcga
     1681 tctctacaga aaactcaaaa gcggccctac ttcttccatg gaaaccggaa tgcaggacac
     1741 tcgctcctct ggacagggga ccctgggtgt caaagaagtg tctttcccat ctctctcttc
     1801 ttctggagcg ggccccttgc aaccgaacat gaatctgctg cttgacgtcc agatgactct
     1861 caccgtagag ctcgggcgca caaagatgtt catcaaagac atcctgggcc tgggagaagg
     1921 atccatcatc gaactggaca agcttgcggg tgaaccggta gacctctatg taaacgatcg
     1981 cctgattgca cgcggggaag tcgtggtcat tgacgaaaac ttcggtgtcc gcgtcacaga
     2041 catcgtttct cgcgcagaaa gattgaaagc ccaaaaacag ggataattcc cgcttgaact
     2101 tctgaaagca gagtctatga atgcctaatg cgacataaga tccgcatagg cgcattagcg
     2161 ctcctggtcc tcaccgtgcc gattttcggg gaaaaaccac ccaaaacgca aaagccggaa
     2221 actccatccg tagacaatcg tgtgaaggaa atagataagt cctggatggc gacgatccac
     2281 ggcaacccat ctgatccaaa gccagacgct gcaaaaacag acgattcgtc gcctaacgtt
     2341 acaaccacac aggactctgc gagagacaac gcggaccagt ccacggccgc aaaaccaggc
     2401 agtgcagaaa aacctgatga aaatccgaca cgacttcgca atccgggcag cgatccaaag
     2461 tcattattc
//
LOCUS       DAORFF010000583         2451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_31467_length_2451_cov_3.697830, whole genome shotgun sequence.
ACCESSION   DAORFF010000583 DAORFF010000000
VERSION     DAORFF010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31467_length_2451_cov_3.697830"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PKK76_17740"
     CDS             complement(<1..509)
                     /locus_tag="PKK76_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70375.1"
                     /translation="MTRLLPITSIFFFVYYLSFITGRPGLEQPEKYEDAKNTSSFKVA
                     FKPGLIERALIQKKAIGLEDDLLESDAQPERLFRSLERSYAGDLFSSAAITLWCTGFL
                     LCFLALRKRVWFGRSMAWLLILPSTVSILIVLFTLRRAGLTGVIVSYDSLAISYVETA
                     LVLIGSASL"
     gene            623..1948
                     /locus_tag="PKK76_17745"
     CDS             623..1948
                     /locus_tag="PKK76_17745"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNL70376.1"
                     /translation="MLRTVILEIFIAARYLRGQGRMVLFSLGTRLSFMFMSFMVYTMV
                     LVLSVFNGFQSEVHKSLWNSGYHVNISRTGNPMFGFEAITQAVLKSKIVSPHVKSAFG
                     SIQVNGLLEVQGRFEGKGIRGIPTEKSGLQTGHLKDFPEIVHYNKQYLEQFDQGNYII
                     IGREMARFYGWQVGDTVHLVLPQGGSLRKGMNIRREEFIIAGFFRTGFYEFDMNLIYM
                     ALPTAQRVLDMPLQVTEVIVQLNDLSSLDFVRSTVRDELPENPYLYTIRTLKDERGNF
                     LAALQLEKVLMIIIMGLLILAGAAGIWVTVHLLVQSKSKSIGMLRAMGLPTRSVLVIF
                     TAHSMLIGLLATLVGGSIGIYAANRLEVTMQLVEDLMNSGCQIVFEHCSPIQLIPKNI
                     YYFDHLPVYADLSVIFGVAIATMILSGLAGYFPSRRAATQDPVMVLRNE"
     gene            1945..>2451
                     /locus_tag="PKK76_17750"
     CDS             1945..>2451
                     /locus_tag="PKK76_17750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNL70377.1"
                     /translation="MMAKEETRSVIVETHNLSKEYRGEGETVRVIDDLDLVVYEKDMV
                     CVEGASGVGKSTLLHVLGTMDSATGAAFVLGRDVLKMSEPEKEMFRARDLGFIFQHHY
                     LLPDFTVLENVMMPLPIQGESFAVADQMSREMLGHVGLGHRLSHFPSQVSGGELARTG
                     VARALVGGK"
BASE COUNT          615 a          586 c          625 g          625 t
ORIGIN      
        1 atcaaagaag cagatccgat caacaccagg gctgtttcta catatgagat ggcaagagaa
       61 tcgtaagata caatcactcc cgtgagacca gcgcgtctga gagtgaacag gacaatcaaa
      121 atagaaactg tggaaggaag aatcaaaagc caggccatag agcgaccgaa ccagactctc
      181 tttcgcagtg cgaggaagca gagtaggaat ccggtgcacc acaatgtgat ggctgcactt
      241 gaaaaaagat ctcctgcgta ggatctttcg agggatctaa acaatctttc tggttgcgca
      301 tctgattcta aaagatcgtc ttccagaccg attgcttttt tctgaatcaa agctctttca
      361 attaaacccg gcttgaaggc cactttgaat gaagaagtgt ttttcgcgtc ttcgtatttc
      421 tccggttgct caaggccggg tctgcctgtg atgaatgata agtagtacac aaagaaaaag
      481 atgctggtga taggcaaaag ccgggtcatt ctgcaatcct tcgatcgtca gtctgcaggt
      541 aaattctttt caatgccggc gcccgagcct cctggagttc catgttgacc gcagactgga
      601 gctttcggaa actgcacgcg aagtgcttcg aacagtcatt ctagaaatat tcatagcggc
      661 gcgctatttg cgcggccagg gccgcatggt cctgtttagc ctcggaacca ggctctcctt
      721 catgttcatg tcattcatgg tctacaccat ggtgcttgtg ctttccgtat tcaatggctt
      781 tcagtctgaa gtgcataagt ccctctggaa tagcggttat catgtaaata tttcgcgcac
      841 cggaaatcca atgtttggat ttgaagcaat cactcaggca gtcctaaaaa gcaaaatcgt
      901 ttctcctcac gtgaaatctg cattcggaag catccaggtg aacgggcttc tagaagtgca
      961 gggacgtttt gaaggtaagg gaattcgcgg aattccaaca gagaaatcag ggcttcaaac
     1021 agggcatttg aaggattttc ctgagatcgt tcactacaat aagcagtatc tcgagcaatt
     1081 cgatcaggga aactacatca tcatcggccg cgagatggct cgcttctacg gatggcaggt
     1141 aggggatact gtgcatctgg ttttgccgca gggcggatct cttcgcaaag gaatgaacat
     1201 tcgcagggaa gagttcatca tcgccggctt ctttcgcacg ggcttttacg aattcgacat
     1261 gaacctgatc tacatggcgc ttcctacggc acagagagtg ctggacatgc cactccaggt
     1321 tacagaagtc attgtgcagt taaacgatct gtcttctctt gatttcgtac gcagcacagt
     1381 gcgcgatgag ctgcccgaga atccgtatct gtataccatt cgcactctca aagacgaaag
     1441 aggaaatttc ctcgcggcgt tgcagctgga aaaagttctt atgatcatca tcatgggact
     1501 cttgattctc gcaggcgcgg ctggaatctg ggttacggtc catctactcg ttcaatccaa
     1561 aagcaaaagc atcggtatgc tgcgcgccat gggtcttccc actcgttctg ttcttgtgat
     1621 ctttaccgca cactccatgc tcattggact cctcgctact ctggtgggtg gctccattgg
     1681 aatctatgct gccaatcgcc tcgaagtcac catgcagctt gtagaagatc tgatgaattc
     1741 aggttgccag attgtgtttg agcattgctc cccgattcag ttaataccta agaatattta
     1801 ctattttgat caccttcccg tatatgcgga cttaagcgta attttcggtg tagccattgc
     1861 caccatgatc ctcagcggac tggccggtta ctttccatct cgtcgcgcag ctactcagga
     1921 tccggtgatg gttctcagga acgaatgatg gcaaaagaag aaacaagatc cgtcattgta
     1981 gagacacaca acctttccaa agagtatcgt ggggaagggg aaacggttcg agtcatagac
     2041 gatctggatc tagtggtata tgaaaaggac atggtatgcg tagagggcgc cagtggcgtc
     2101 ggcaagagca cactgttgca tgtcctgggc actatggatt ctgccacagg tgccgccttt
     2161 gtgcttggac gagacgtgct caagatgagt gaaccagaaa aagaaatgtt tcgcgctcgc
     2221 gatcttggct tcatcttcca gcatcattat ctgcttccag acttcacagt tctggaaaac
     2281 gtcatgatgc ccctgcccat tcagggagag agttttgccg tcgcagacca gatgtcccgc
     2341 gaaatgctgg ggcatgtagg gcttggccac aggctgagcc actttccatc tcaggtatcc
     2401 ggcggggaat tggctcgaac cggggtggcc cgggcgctcg tggggggcaa a
//
LOCUS       DAORFF010000584         2442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_31679_length_2442_cov_2.890658, whole genome shotgun sequence.
ACCESSION   DAORFF010000584 DAORFF010000000
VERSION     DAORFF010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31679_length_2442_cov_2.890658"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /locus_tag="PKK76_17755"
     CDS             <1..1601
                     /locus_tag="PKK76_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004756774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HNL70378.1"
                     /translation="SGGLHGVGVSVVNALSEWLEVEVHRDGQIHVMSFERGKVKNPAK
                     VKGKTNRTGTTTRFKADNTIFTTTEYKYALLHQRLEEMAYLNAGLKMELRDERKQPAK
                     KEVFEFAGGIKEFVARHLQKKQKLHKHIIAFEGKNDKLIGEFAMAYTDSQNEHIFCFT
                     NGINNNLGGTHLEGFRTALTRTLNEYVKKDESMKKKLGGTSLTGDDVREGLVAVISVK
                     VPEPQFNSQTKEKLVNAEVKGLVQQIVGDKLSLFLEENPGEIKPILEKCVLSFRAREA
                     ARKARELVTKRKGVLESGGLPGKLADCSERNPELCELFLVEGDSAGGSAKQGRNRHYQ
                     AILPLKGKILNVIKGSDDKILENEEIRTLVTAIGIGFGNDGLDLDKLRYHKIIIMTDA
                     DVDGSHIRTLLLTFFYKKMRPLVDNSFLYIAQPPLYLIKNGKDAHYAFTDQEKDKIVK
                     KYNSDKTVVQRYKGLGEMNPEQLWETTMDPEKRVMLKVRPLSESEVGLDGIERTFDIL
                     MGDDVEPRRRFIEQNARLVAHLDL"
     gene            complement(1622..>2442)
                     /locus_tag="PKK76_17760"
     CDS             complement(1622..>2442)
                     /locus_tag="PKK76_17760"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL70379.1"
                     /translation="FTLDDDLRFTSVNRALHRDLGFPEEEVLGSRFFDLLYQGELESG
                     LAVNLVHRRFEEFKRNGKTLGMKIQLKSKYTQEPREFSARFERVQVDDGIQILGKATL
                     VLEDSLLRYFIKERQKYVIGNYLISAEELSQRLVRNVEKYLDPSRVTALRIGLREILI
                     NAIEHGNLAITYDEKTRELTTGNYLEFIQARQLDPRFRDRKVVIEYELNTLFVQYTIT
                     DEGEGFDWQKLKAEEDIHQQDSHGRGIAVTENAFDELIYSGKGNQVQLIKHFRR"
BASE COUNT          647 a          580 c          616 g          599 t
ORIGIN      
        1 tttctggcgg tcttcacggt gtgggcgttt cagtcgtcaa cgcgctgtct gaatggctgg
       61 aagtagaagt tcatcgcgat ggacaaattc atgtaatgtc atttgagcgc ggcaaggtaa
      121 agaacccggc caaagtgaaa ggcaaaacaa atcgcactgg aaccaccact cgcttcaaag
      181 cagacaatac aatcttcacc actacagaat acaaatacgc acttctgcat caacgcctcg
      241 aagaaatggc gtacctcaat gccggcctga agatggaact gcgcgatgag cgcaaacaac
      301 cggcaaagaa agaagtattc gagttcgcag gtggcatcaa agagttcgtg gccaggcatt
      361 tgcagaaaaa gcaaaagctt cacaagcaca tcatcgcatt tgaaggcaaa aacgataagc
      421 tgatcggtga attcgccatg gcgtacactg attcgcagaa cgaacacatc ttctgtttta
      481 caaacggcat caacaacaac ctgggtggaa cacacctgga aggctttcgc actgctctga
      541 ctcgtactct caacgaatac gtgaagaaag atgaatccat gaagaagaag ctcggcggaa
      601 cttctcttac tggagatgac gtgcgcgaag gtcttgtggc tgtgatctcc gtaaaggtgc
      661 cagagcctca gttcaactcg caaacaaaag agaaactggt aaacgcggaa gtcaaaggac
      721 tcgtgcagca gatcgtcgga gacaagctgt cattgttcct ggaagaaaat ccaggtgaga
      781 tcaaaccaat tcttgagaag tgcgttcttt cttttcgcgc tcgcgaagca gcccgtaagg
      841 cgcgcgaact tgtaaccaag cgcaagggcg tactggagag cggtgggctt ccaggaaaac
      901 tggcggactg ctctgaaaga aatcctgagc tgtgtgaact cttcctcgtg gaaggtgatt
      961 ctgcgggtgg atctgcaaag cagggtagaa atagacatta tcaggccatt cttcctctca
     1021 aaggaaagat cctcaatgtg atcaagggaa gtgatgacaa gattctggaa aatgaagaaa
     1081 tcaggactct tgtaactgcc attggaattg gttttggaaa tgatggtctg gacctggaca
     1141 agcttcgcta tcacaagatc atcatcatga cggatgcgga cgtggacggt tcgcacatcc
     1201 gcactctctt gctcactttc ttctacaaga agatgcgtcc tcttgtggac aatagctttc
     1261 tatacattgc gcagcctcct ctctacctga tcaagaacgg gaaggatgca cactacgctt
     1321 ttacggatca ggaaaaagac aagatcgtca agaagtacaa ttccgacaaa accgtggtgc
     1381 agcgttacaa aggtctgggt gaaatgaatc cggagcagct ctgggaaacc acaatggatc
     1441 cagagaagcg cgtgatgctg aaggtgcgtc cactttctga atctgaggtt ggcttggacg
     1501 gaatcgaacg cacgtttgat attctgatgg gcgatgacgt ggaaccacgc agacgcttca
     1561 ttgagcagaa cgccagactg gtggctcacc tggatctttg attggatact gaaccggcgc
     1621 ttcagcgccg gaagtgtttg atgagctgca cctggtttcc tttgcctgaa tagatcaatt
     1681 catcaaatgc gttttctgta actgcaattc cccggccgtg gctgtcttgc tggtgaatgt
     1741 cttcttctgc cttgagtttc tgccagtcaa agccttcccc ttcgtctgtg atcgtgtact
     1801 gcacaaacag ggtatttagt tcgtattcga tgaccacctt acgatcgcga aagcgagggt
     1861 cgagctggcg cgcctgaatg aattcgagat agtttccggt tgtcaattcg cgcgtttttt
     1921 cgtcgtaggt gatggccagg tttccgtgtt cgattgcgtt gattagaatt tcgcgcaggc
     1981 caatgcgaag tgcagtcacg cgcgaaggat ccagatattt ctccacgttt cgcactagtc
     2041 tctggcttag ctcttccgcc gaaatgaggt agtttccgat cacgtacttt tgtctttctt
     2101 tgataaaata acgaagaagc gaatcttcca ggaccagagt tgctttccca aggatttgaa
     2161 ttccatcgtc tacctgcacc cgttcaaatc gagcgctgaa ttcgcgaggc tcttgcgtgt
     2221 atttggactt cagctggatt ttcatcccca gcgtttttcc atttctcttg aactcttcaa
     2281 agcgacgatg aaccagattc acagccagcc ctgattcgag ttctccctgg tagagcaggt
     2341 caaaaaaccg cgagccgagt acttcttcct ccggaaatcc caggtctctg tgcaaagctc
     2401 tgtttacact cgtgaagcga agatcgtcat cgagagtgaa ga
//
LOCUS       DAORFF010000585         2441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_31696_length_2441_cov_3.976949, whole genome shotgun sequence.
ACCESSION   DAORFF010000585 DAORFF010000000
VERSION     DAORFF010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31696_length_2441_cov_3.976949"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKK76_17765"
     CDS             complement(<1..333)
                     /locus_tag="PKK76_17765"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNL70380.1"
                     /translation="MRVVVTGGAGYIGSHTVLALLEAGHDVVVVDDLSTGHKINLPDG
                     VKFVPGRFQDDSVIQAALSESPDALFHFAALKAAGESMEEPEKYAAANIRGSLGLIER
                     CAASGVRNF"
     gene            451..648
                     /locus_tag="PKK76_17770"
     CDS             451..648
                     /locus_tag="PKK76_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70381.1"
                     /translation="MENKEETSMESLVVASKAKLIIKNHDCMVAGDALDELNRKVHRL
                     IEDAVKRTKDNKRKTVQAYDF"
     gene            658..1041
                     /locus_tag="PKK76_17775"
     CDS             658..1041
                     /locus_tag="PKK76_17775"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNL70382.1"
                     /translation="MPPGVRVVKWPHKETPDEESVKKEMAGLGYRVYDLQTIPPWFER
                     SRHSHDYDEIRGAVEGVTTFHFDDGPVTLEAGDILFIPAGVPHEVRTHNDRPFTAYKA
                     SISGERVVTEHGDGKGSVEDLQARS"
     gene            1038..1673
                     /locus_tag="PKK76_17780"
     CDS             1038..1673
                     /locus_tag="PKK76_17780"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNL70383.1"
                     /translation="MIAFFDLDGTLEDSRQDMALSINRVRETKSLPALSLEVARSLVN
                     RGMDALTEAAFPEIIENQTDLKKVRALYEEDYLSHVVDHTRLYDGIASMLQGVASIGK
                     LVVYTNKPERISRELLKRLQVLETVSGIIGGDSFPESKPSPIPMQTMADRLGETGARW
                     MTGDTAADMQAARNFGAKSIWASWGYISSAPEPSPDFVAKVPSDVLEFIRN"
     gene            1723..2091
                     /locus_tag="PKK76_17785"
     CDS             1723..2091
                     /locus_tag="PKK76_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70384.1"
                     /translation="MKKIAWISLVIVACFASQSCRSSQLAFTEDTTKYNGMVPVDGPS
                     GIMQKTTVIGVHLLFGLIPVYRASLDSALDSFTKDARKRGASRVRVISATRETGIWYL
                     PPFTFFITPVFFDMTGEIYR"
     gene            complement(2143..>2441)
                     /locus_tag="PKK76_17790"
     CDS             complement(2143..>2441)
                     /locus_tag="PKK76_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70385.1"
                     /translation="MEMMAGQLTIYGASLLPTLRSLAESSEEASRAVGIQTLCNMYFE
                     HKLPHPDEEFLYSQIKDFKDDRFATGHLAELVRDDIQSRKGEDSERLLKDIIGL"
BASE COUNT          641 a          646 c          622 g          532 t
ORIGIN      
        1 aaaattgcgt acgccggacg ccgcacatct ctctatcaat ccaaggcttc cccgaatgtt
       61 ggctgctgca tatttttcag gctcttccat ggattccccg gctgctttca aagccgcaaa
      121 atggaacagc gcgtcaggcg attcagaaag ggctgcctgg atcactgagt cgtcctgaaa
      181 tctgcctgga acgaatttca ctccatccgg caggttgatt ttgtgacctg tggacaggtc
      241 atccactacg actacatcat gaccggcctc tagaagggcc agaacggtgt gggagccaat
      301 gtagcctgcc ccgccagtaa cgacaactcg catggcacca gagtcctcca gacccccttc
      361 cctgtgaaac ggaattccag gaggggtggg attttgaatt gatgcgtcga aaagacggtt
      421 cagtcttgcc gaaaggagaa atctctcggt atggaaaaca aagaggaaac ttcaatggaa
      481 agcctggtcg ttgcgagcaa ggcaaagctc atcatcaaga accatgactg catggttgct
      541 ggagatgcgc tcgatgaact gaatcgcaaa gtccaccgtc tcattgagga cgcagtaaag
      601 cgcacgaaag acaacaagcg caagacagta caagcatacg atttttgagg tttcccaatg
      661 ccccccggag tcagagtagt aaaatggcca cataaggaaa caccggatga agaatcggtg
      721 aaaaaggaaa tggccggtct aggatatcgt gtttacgatc ttcagaccat tccgccgtgg
      781 tttgagcgaa gccgccattc acacgactac gatgaaatcc ggggcgcagt ggaaggcgtc
      841 acaacatttc attttgacga tgggcccgta acactggaag cgggcgacat cctcttcatt
      901 ccagctggag tcccgcacga agtgcgcacg cacaacgatc gtcccttcac agcctacaaa
      961 gcatcgatct caggtgaaag ggtagtaacc gaacacggag acggaaaagg cagcgtagaa
     1021 gacctgcaag cacgaagttg atcgcattct ttgacctgga cggcacactc gaagactcga
     1081 gacaggatat ggctctgtct atcaatcgag tgcgcgaaac aaaatcactg cccgcactct
     1141 cgctcgaagt ggctcgctcc ctggtaaacc gcgggatgga tgcccttact gaggctgcct
     1201 tcccggaaat catcgagaat caaacggacc tcaaaaaagt gcgagccctc tacgaagagg
     1261 attatctgtc gcacgttgta gatcacacca gactctatga cggcatcgct tcgatgctgc
     1321 aaggcgtggc atcgattgga aaactcgtgg tctatacaaa caaaccggag agaatctcaa
     1381 gggaactctt gaaaagactg caggtcctgg aaaccgtttc aggcatcatt ggaggagatt
     1441 ccttccccga aagcaaacca tccccgattc ccatgcaaac catggcggac agactgggtg
     1501 aaacaggtgc aaggtggatg accggagaca cggcggcaga catgcaggcg gcgcgcaact
     1561 tcggcgcaaa aagcatctgg gcttcctggg gttatatctc ctctgctccc gaaccttcgc
     1621 cggatttcgt ggcaaaggta ccatccgacg ttctagaatt cattcggaat tgatctggaa
     1681 ttttcaattg cagaaagacc ggcacactcc agtgtccgct ccatgaaaaa aatcgcctgg
     1741 atctccctgg taatcgtggc ctgcttcgct tcgcagagct gcagaagctc ccagcttgcc
     1801 ttcacagaag acactacaaa atacaacggc atggttccag tagacggtcc atccgggatc
     1861 atgcagaaga caaccgtcat aggcgtgcac cttctatttg gtcttatccc ggtctataga
     1921 gcaagcctcg attcagcact cgatagtttt acaaaagatg cgcgcaagcg cggagcaagc
     1981 cgcgtaaggg tcatcagtgc cacgcgtgaa actggaatct ggtatctgcc accatttact
     2041 ttctttatta cgcccgtctt ctttgatatg acgggtgaaa tttaccgtta agcgtcattc
     2101 gttgggggcc cctgcatctc acaggggcgg cccgaacggc gatcataagc caatgatgtc
     2161 tttgagcagc ctttcgctgt cttcgccttt tcgcgactga atgtcatctc tcaccagctc
     2221 tgccaggtgc ccggttgcaa agcgatcatc cttgaagtct ttgatctgag aataaagaaa
     2281 ctcttcgtct ggatgaggaa gtttgtgttc aaagtacata ttgcacagag tctgaattcc
     2341 aaccgcgcgt gaagcctcct ctgacgattc agccagactt cgcagagtgg gaaggagact
     2401 tgcgccatag atggtgagct gacccgccat catttccatt g
//
LOCUS       DAORFF010000586         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_31965_length_2430_cov_3.080000, whole genome shotgun sequence.
ACCESSION   DAORFF010000586 DAORFF010000000
VERSION     DAORFF010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31965_length_2430_cov_3.080000"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2158)
                     /locus_tag="PKK76_17795"
     CDS             complement(<1..2158)
                     /locus_tag="PKK76_17795"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNL70386.1"
                     /translation="MHLLDAYVSQYQPFSAGKESLPAAGHAFLIAAALSQPGKKGIII
                     VAPDDAQARRLCDEVKSLCGKGTLLPSRRILPYEMVRVSRDVEGEREQGLSALAHGKP
                     VFTTADALCERFYPSRDQLTLKVNDEIGPDDLAASLVRLGYRPSGRTEVPGEFTRKAG
                     IIDVFASNLPEPIRIDYFDTIIESIRKFDAESQIGGEKLINVEIGSAAETILSESQSK
                     DLREKLKKSFKSEILPPWIDEEFGVINSRDCESLHEFLPLILEGKTLSELAGKKPLLI
                     MADCDSVRARLDLFEREVRECFAAASSTHACVPCEKAYHFSPLDGFEVLEIEDSEFPG
                     DCGIQRLEGVKGRIADIQKAVGKLVLEGQKVFITAPDRTQLDRIAGVFRSQTDYKTES
                     VVDFQMDAKKRGLVSLVVSPLREGIRIPSLSTQILTDADLFGRGYKKKSYFKKRKSSP
                     IESFLDLKEDDHVVHVNHGIGKFIGLERVSAAGRERDFLVIEYADKDRLFVPLDQISL
                     IQRYSAPTEEPRLDSLGKASFKKIRERVAERTEQLAQELVNIYAARMKTPGYPFPADT
                     IYQDEFEGAFEWEETPDQLAAIESVKADMEAPRPMDRLICGDVGYGKTEVAIRAAFKA
                     VMAGKQVVFICPTTVLALQHFRTFSKRYAEYPIKVDWMSRFRTAKEVADVKRRATSGE
                     VDVVIGTHSLLASDVRFKNMGLLVIDEEQRFGVAHKE"
BASE COUNT          584 a          655 c          556 g          635 t
ORIGIN      
        1 cttctttgtg tgccacacca aagcgttgct cttcgtcgat caccagaagt cccatattct
       61 taaagcgaac gtcacttgca agaagcgagt gagtgcctat gaccacatcc acttccccgg
      121 acgtagccct tctcttcaca tccgccactt ccttcgcagt acgaaaacga ctcatccagt
      181 ccactttgat cgggtattcc gcataacgtt tggaaaacgt gcgaaagtgc tgcagagcaa
      241 ggacagtcgt cgggcaaatg aagacgactt gcttgcctgc catcaccgct ttaaaggcgg
      301 ctcggatggc tacttctgtt tttccgtatc ccacatctcc gcagatcaat cgatccatag
      361 gccgtggagc ttccatgtcc gccttcactg attctatggc tgccagctga tctggcgttt
      421 cttcccactc aaaggcacct tcaaattcgt cctgataaat tgtatctgcc gggaagggat
      481 atcccggagt tttcatgcgc gcagcataga tgttgacgag ttcctgagcc agctgctctg
      541 ttcgctcggc caccctctct ctgatctttt taaaagatgc tttgccgaga gaatccagcc
      601 ttggctcttc ggtgggcgca gaataccttt gaatcaagga tatttgatcc agaggcacga
      661 acagtctgtc tttgtctgcg tactctatga ccagaaagtc gcgctctctg cccgctgcac
      721 tgacgcgctc aaggccaatg aattttccaa tgccgtgatt tacgtgaact acatgatcgt
      781 cctcttttag atccagaaag gattcaatgg gagacgattt tctttttttg aaataggatt
      841 tcttcttgta ccctcggcca aacagatccg cgtccgtaag tatctgagtg gaaagggagg
      901 gaatgcgaat tccttcgcgc aagggagaaa ctacaagaga taccagacct ctcttcttcg
      961 catccatctg aaaatcaaca acgctttccg ttttatagtc tgtttgagat cgaaaaacac
     1021 ccgctatgcg atctaactgt gtgcgatccg gtgcagtgat gaagactttc tggccttcca
     1081 ggaccagttt cccaactgct ttttgaatat ctgcgattct gcctttgact ccctcgagac
     1141 gctgtatgcc acaatcgccc ggaaattctg aatcctcaat ctcaagaacc tcaaagccgt
     1201 ctaacggtga aaagtgatat gctttctcac agggaacgca ggcatgtgtg gagcttgccg
     1261 ccgcaaagca ttcgcgcact tctcgctcaa acaaatccag acgcgcgcga actgaatcac
     1321 aatctgccat aatcaagagt ggctttttgc ccgcgagctc tgagagagtt ttgccttcga
     1381 gtatcagggg aagaaattca tgaagtgatt cgcaatcgcg agagttgatc acaccaaact
     1441 cttcatcgat ccatggtggt agaatttctg acttaaagga cttctttagt ttttcgcgca
     1501 gatccttcga ttgactttca gacagaatcg tttctgccgc agatccaatc tccacattga
     1561 tgagtttttc cccgccaatc tgactctctg catcaaactt gcgaatgctt tcgatgattg
     1621 tatcaaagta atcaatacga atcggctctg gcagattgga cgcaaacaca tcgatgatgc
     1681 ctgcctttcg cgtaaattct cctggaacct ctgttcggcc agaaggcctg taccccagac
     1741 gaaccaggga tgcggccagg tcatccgggc caatctcatc gttcactttc agagtgagct
     1801 ggtctctaga tggataaaat cgttcgcata gcgcatccgc agtcgtgaac acaggttttc
     1861 catgagccag agcggaaagc ccctgctctc tctctccctc cacatctcgt gatactcgca
     1921 ccatctcata ggggagaatc cgcctggaag gaaggagagt tccttttccg cataatgatt
     1981 ttacttcgtc gcacaatcgg cgggcctggg cgtcgtcggg ggctacaatg atgattcctt
     2041 tcttgcctgg ttgactgagt gctgcggcga tgagaaacgc gtggccggcc gccggtaaag
     2101 attccttacc cgcggaaaat ggttggtact gcgagacata ggcgtcgagc agatgcaagg
     2161 caggccagag tccaattcat gggtcctcgt gtcacttgat ttaggtccgc aattccactc
     2221 tggcccgcaa gatcggctct aaactgaccg aaaataacat tgactcaact tgatccgagg
     2281 agatctttcg gccatgacca tcttccgcac acttgcactt tccattgtgc tcattccttc
     2341 tataatgttc gctcaagaga aggacctgaa cgattctaag aaaccccgcc tgtccgaaga
     2401 ggaaatccgc acgggcaaag tagaattcat
//
LOCUS       DAORFF010000587         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_31986_length_2429_cov_3.759478, whole genome shotgun sequence.
ACCESSION   DAORFF010000587 DAORFF010000000
VERSION     DAORFF010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31986_length_2429_cov_3.759478"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..745)
                     /locus_tag="PKK76_17800"
     CDS             complement(155..745)
                     /locus_tag="PKK76_17800"
                     /inference="COORDINATES: protein motif:HMM:NF020906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HNL70387.1"
                     /translation="MMMLRAPRESDVLRFLEVSATDRFSYEETGMSQGAPGELTLSGR
                     YNLDYNRQYLGTGPAIFERAKAAIRQWKMFDLSWIDLVPLETPLEAGRTVAVVARHLG
                     FYSLNASRILYTVEETGEMERFGFAYGTLTSHSEIGEERFLVEYDRKTGQVHYDLFAF
                     SRPGLLAWIGYPVSRTLQKRFARESREAMVRAVAAP"
     gene            880..1632
                     /locus_tag="PKK76_17805"
     CDS             880..1632
                     /locus_tag="PKK76_17805"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNL70388.1"
                     /translation="MSTLILVFAVQAFCFIFASVFQTDKLTDFAYGMTFVLLASIFLL
                     QAGENTLSWILCSCIILWGLRLSGYLFVRILKIGKDERFDGRRENFWRFAFFWIFQAI
                     VIWVILLPVIVAVQSSAQPGVLSYAGVAVFLFGLVYETIADAQKFQFRNRPENKGKWI
                     ESGLWKYSRYPNYFGEVVLWWGVFLIAIPALSAAAWFTIAGPICITFILLKVSGIPLL
                     EEGHKKRYGKNPAWLAYAKRTSTLFPWFPEKQ"
     gene            1629..>2429
                     /locus_tag="PKK76_17810"
     CDS             1629..>2429
                     /locus_tag="PKK76_17810"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL70389.1"
                     /translation="MKLRLGAGYGAAETGIAAAEVMLQLYLLNFYIGQGLSPFLTGLA
                     LACAVALDAFADPLVGGISDRARPEMGRRRAFILGGSIFLSIAMPALFHPPSTGEAGL
                     FAYLLISYLGVNLAMTLLSVPHSAMAGELVPDPSERTALFGFRLLFNCVGLLAGIISP
                     SLSGSSGHGSIVIGVIALVSALITFRATRGLDVLPAKPESNVPRQPMLDVMRSTLSNR
                     SFLLLVLAFLIASVGRTFNSSLALYYYKYRLLLSEEQTTRVVLGLFVLV"
BASE COUNT          560 a          641 c          574 g          654 t
ORIGIN      
        1 caccagtcct agaagtataa gtttcttcat cggtcactcc ccccactgag tgttgagaca
       61 tttgctgaca ccgtgtcaca aaagtcaacc cgaattcggt gcaatgcaca aaaaaaaaga
      121 gcaattccat ccgaaaaaag caaaaacgcc cgccttacgg agctgccaca gcccgaacca
      181 tggcctctct ggactcgcga gcgaatcttt tttgaagagt acgactcact ggatagccaa
      241 tccatgcaag tagtcccggt cgcgagaaag caaagagatc gtagtggacc tgcccggttt
      301 tgcggtcata ttcgaccaga aaccgctcct ctccgatttc gctgtgcgaa gtcaacgtgc
      361 cataggcgaa accaaatcgt tccatttccc ctgtttcttc tacagtatag agaattcggg
      421 atgcgttgag tgaatagaac cccagatgtc tggccacgac ggcaacagtc cgacccgcct
      481 ccagaggcgt ttctagcgga accagatcaa tccagctcag atcaaacatt ttccattggc
      541 gaatcgctgc ctttgcgcgt tcaaaaatag ccggccccgt tccgaggtac tgtcgattgt
      601 agtccaggtt atagcgaccg gaaagagtga gttccccagg agctccttga ctcatcccgg
      661 tttcctcata cgagaaacgg tctgtagcag aaacctcaag aaatctcagc acatccgatt
      721 cacggggagc acgcagcatc atcatccgat ttggccttcg acatattgtc caatgttttt
      781 gcgtttccta cgacaaaata tagagacagc ggaacgcatt tccattagtt taccaggatg
      841 cacttcctgc ctttcttttc tttcgattcc tggaacctgt tgtctaccct cattctggtg
      901 ttcgcagtcc aggcattctg tttcatcttt gcatctgttt tccaaacaga caaactgact
      961 gattttgcgt acggaatgac ctttgttcta ctcgcgtcta tttttctttt gcaggcaggc
     1021 gaaaatacgc tgtcatggat tctttgcagt tgcatcatcc tgtggggctt gagactttcc
     1081 ggatatcttt ttgttcgaat tctgaaaatc ggcaaagacg aacgatttga cgggagaaga
     1141 gagaatttct ggagattcgc attcttctgg atctttcaag cgattgtcat ctgggtcatc
     1201 ttgcttccag tcatagtcgc cgtgcaatca agtgcacaac ctggcgtctt gagctatgcg
     1261 ggcgttgctg tgtttctctt tggcctggta tacgaaacta tcgcggacgc gcaaaaattc
     1321 caatttcgca acaggcccga aaacaaaggc aaatggatag agagcggact ctggaaatac
     1381 tcaagatatc ccaactactt tggtgaagtc gttttgtggt ggggagtttt ccttattgcg
     1441 atccctgcgt tatccgcagc agcatggttt acaatcgcag gtcccatttg catcacgttc
     1501 atccttctga aagtgagcgg cattccgctg ctggaagaag gtcacaaaaa aaggtacgga
     1561 aagaatcctg catggctggc ctacgcaaag cgaacgagca cactcttccc gtggtttcct
     1621 gaaaagcaat gaaactgcga ctcggagcag gttacggagc ggccgaaaca ggaatcgctg
     1681 cagcagaagt tatgctgcag ctctatcttc tgaatttcta tattggccag ggattgagcc
     1741 cttttctgac tggactggca cttgcctgtg ccgtagcgct ggatgcgttt gcggacccgt
     1801 tagttggcgg gatctctgat agagctcgtc cggagatggg cagaagacgt gcattcattc
     1861 tgggtggctc catctttctt tccattgcga tgcccgcact ctttcatcca ccttccacgg
     1921 gagaagcagg gctgtttgca tatctcttaa tttcctacct cggggtcaac cttgccatga
     1981 cccttctctc cgttcctcac agcgcgatgg cgggggaact tgttccggat ccatcggaaa
     2041 ggactgcact atttggattt cgccttttgt tcaattgcgt cggactgctg gctggaatca
     2101 tttctccttc tctttccgga tcttccggcc atggtagcat tgtcattgga gtgatcgcgc
     2161 ttgtctctgc gctcatcaca tttcgcgcga cgagaggtct ggacgtccta ccggcaaaac
     2221 cagagtcgaa tgtgccgcgc cagcccatgc tggacgttat gcgatctacg ctgagcaacc
     2281 ggtcatttct cctcctggtt cttgcgtttc tgatcgcctc agtgggtaga acattcaact
     2341 cctctctggc tctctactac tacaaataca gactcttact ctcagaggaa caaacaacac
     2401 gcgtcgtcct gggactcttt gtacttgtg
//
LOCUS       DAORFF010000588         2424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_32121_length_2424_cov_3.255804, whole genome shotgun sequence.
ACCESSION   DAORFF010000588 DAORFF010000000
VERSION     DAORFF010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32121_length_2424_cov_3.255804"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1714
                     /locus_tag="PKK76_17815"
     CDS             143..1714
                     /locus_tag="PKK76_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70390.1"
                     /translation="MKTRLAVGSFLVACAIFAILRNAEADPAILSETAYPSVAWKDFS
                     PTIDLSSPPALRQTESIDLWDGMIFAKDRVVLPLRTAGGRVLWSWEPGKLPVRSEIQG
                     DIQSIAYDDGKDEILILTTNKLLFLRRSSLAILRETPTSIQASDMGVSGKSLIARKGN
                     DFTVYSLPDLQKLRTFSAPQPQFTQNKDPQRAIYLADGTILLNSTYWGAKLLRFHPDR
                     PEISREESISIPHRALLRFAPAGKDDFVSFDPLTSTYAAYRYANGHYYEMTKGMQEFE
                     NFSAVRWAPREQKIKMEVIIETDASAKLAELLVLLPPGETYSQTLSGEALVQGSEIIR
                     DRHGNRYAKLFVRLEAGVSRVIVYAATLRRYLFQADLTRFEDQLSRRQYPGFLKNYTA
                     DYKEFEIHNPIVESKKQEILSQAGASSRLFLEKTLSAAAAIPYKGDGRFDPAPVIISQ
                     NHGTYIFHKRDFARKRSTRENRLGLAASGGRHRIQSQDSGNVASWIWLDPDGIAQPSA
                     RSRNHIRSISGFRCL"
     gene            1644..1877
                     /locus_tag="PKK76_17820"
     CDS             1644..1877
                     /locus_tag="PKK76_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70391.1"
                     /translation="MESLSPPREAATTYARFLVFGAFDHGAESLTSGGDTLVKLTENG
                     KRLKTKSLRVQITLDPAEPGKSRKIRNTEEKVE"
     gene            1874..>2424
                     /locus_tag="PKK76_17825"
     CDS             1874..>2424
                     /locus_tag="PKK76_17825"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNL70392.1"
                     /translation="MKLDAIVIGAGPIGIEMAAALKKEGLSFAHIERGRIGETIFHYP
                     PATHFFSSPERIAIAGVPLLTQDQTKATREEYLLYLRSVVTQLDLETRLYESVESVTQ
                     AADSFQVQTRHTLTEQKTTYTARNVILATGDMHKPRMLGIPGESLPHVSHYPADPHSY
                     FRTRVLIVGGKNSAAETALRLTRA"
BASE COUNT          676 a          648 c          557 g          543 t
ORIGIN      
        1 tgaaaaacca gctgatcaca atcaaaggtg gaactcacgg cggttttcca gaacccgaat
       61 accttcgcgc gtacagagca gtgtttgcgt tcataggcat tccctgacag tacttcgcaa
      121 aattctacaa ttcgaggctc aaatgaaaac cagactggcc gtcggatctt ttctagttgc
      181 atgtgcgatt ttcgcgatcc tcaggaatgc agaggcagac ccggcgatcc tatcagagac
      241 ggcctatcct tctgttgcgt ggaaggactt ttctcccacc atcgacttgt cctctccccc
      301 ggcactcaga caaactgagt ctatagatct ctgggatggt atgatcttcg caaaagatcg
      361 cgttgtgctt ccactcagaa ctgcaggcgg acgcgtgctg tggtcctggg agccaggcaa
      421 gcttccagtc cgctctgaaa ttcagggaga tatacaaagc atcgcctatg acgacggcaa
      481 agatgagatt ctaatactta ccacgaataa actattgttt cttcgcagat ccagtctggc
      541 catactgcgc gaaactccga ccagcattca agcctcagac atgggggtat ctggcaagtc
      601 tttgatcgca aggaaaggaa atgatttcac agtctattct cttcccgatc ttcagaaact
      661 acgtacattc tccgctccac agccgcaatt cacacaaaac aaggatccac agagagccat
      721 ctatctggcg gatggcacca tcctactgaa ctccacttac tggggcgcga aactgctgcg
      781 atttcatcca gatcgcccgg aaatcagcag agaagaatcc atttcgattc cacacagagc
      841 tcttcttcgc tttgctcctg caggaaaaga tgactttgtt tcctttgacc cactcacctc
      901 tacctatgcc gcctatcgtt atgcaaacgg tcactactac gagatgacaa aaggcatgca
      961 ggaattcgag aacttcagcg cggttagatg ggcaccacga gaacaaaaaa tcaaaatgga
     1021 agtcatcata gaaacggatg cttccgcaaa actcgcagaa ctattggtac tcctgcctcc
     1081 aggagaaaca tattcacaaa cattgtccgg ggaagcactc gttcaaggat cggagatcat
     1141 ccgggataga catggaaacc gttatgcgaa attattcgtc cgcctggaag ccggagtttc
     1201 ccgtgtgatt gtgtatgcgg ctacccttcg ccgctacctt ttccaggcag atctgacgcg
     1261 attcgaagac caactttcca gaagacagta tcctggcttc ttaaagaact acaccgcaga
     1321 ctacaaagaa tttgaaatac ataaccccat cgtggaatca aagaaacaag aaatcctctc
     1381 tcaggccggc gcatcgagca gactctttct tgaaaagact ctttctgcgg ccgcggccat
     1441 cccatacaaa ggagacggca ggtttgatcc tgccccagtc atcatttcgc aaaaccacgg
     1501 aacatacata tttcacaaac gcgattttgc gcgcaaacga tctacccggg agaaccgttt
     1561 gggtctggct gccagtggag gcagacatag aattcaatca caagatagcg gaaacgtggc
     1621 ttcctggatt tggctggatc ccgatggaat cgctcagccc tccgcgcgaa gccgcaacca
     1681 catacgctcg atttctggtt ttcggtgcct ttgatcacgg ggcagaaagt ctaacgtcag
     1741 gcggcgatac tctggtcaag ctcacggaaa acggaaagcg cttgaaaaca aaatctcttc
     1801 gggttcagat cactctcgat ccggcggagc caggaaaatc aagaaagatc aggaacacgg
     1861 aagagaaagt agaatgaaac tggatgcaat cgtcatcggg gcgggaccca ttggcattga
     1921 aatggcggcc gccttaaaaa aagaaggatt gtctttcgca cacatcgaac gcggacggat
     1981 tggagaaact atatttcact atccgccggc cactcacttc ttcagctctc cagaaagaat
     2041 cgccatcgcc ggagttccac ttctcactca ggatcaaacc aaagcaacgc gagaagaata
     2101 ccttttgtat ttacgatctg tggtcaccca attggatcta gaaacaagac tgtatgagtc
     2161 tgtagaaagt gtgacccagg cggccgattc attccaggtg caaactcgcc atactctaac
     2221 ggaacagaaa actacgtata cggcccggaa tgtaattctt gccacaggag acatgcacaa
     2281 acctcgcatg cttggaattc ctggagaaag ccttccgcac gtcagtcact acccggcgga
     2341 tcctcacagc tattttcgca ctcgagtgtt gattgtagga ggcaagaact ctgccgcaga
     2401 aaccgctctg agactgactc gcgc
//
LOCUS       DAORFF010000589         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_32273_length_2418_cov_2.157850, whole genome shotgun sequence.
ACCESSION   DAORFF010000589 DAORFF010000000
VERSION     DAORFF010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32273_length_2418_cov_2.157850"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PKK76_17830"
     CDS             complement(<1..761)
                     /locus_tag="PKK76_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70393.1"
                     /translation="MILTVSIFRLNFCRLKMLPPTLRDYRYQIDEAVNRSHHGGMKSI
                     SRAAKIHLLSAWAIMASIACVSQEMQNAQEICQKRDQSPVQCAAQNFKEGVGMEDSGY
                     YGSAKSAFRIAINLDPEGKNTWRYYAELCAVNYPLGRRAIKHGKQFQWDDLHREVGYD
                     RWLEEALAECDAAVAWAKDEYPDALAARGSLRLLAENKSGACEDFRKAKSIGGGEEPR
                     IYTHDLVTACGSPTYFAFGPPQALRVDRDHLQIILP"
     gene            complement(715..1326)
                     /locus_tag="PKK76_17835"
     CDS             complement(715..1326)
                     /locus_tag="PKK76_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70394.1"
                     /translation="MNEIQVTLTGNMIAFWALEFAGLECQKIRDYCEEHDEAIETFFG
                     GPSEEESVQAALEDDNWDLFSAAAVTDDFGSGGNAFIADEDLSIIVTHASDRIEIPFT
                     HLRQANRRTVSAEDWSASRAGQRILYIFGYRSDSPSAAEFSLGRSDMFSIDRLAIAVC
                     ENQIIGTSFVASISYTHPDGAVTVDATGNDFDGEYFPAEFLQT"
     gene            complement(1326..2111)
                     /locus_tag="PKK76_17840"
     CDS             complement(1326..2111)
                     /locus_tag="PKK76_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNL70395.1"
                     /translation="MENSEAFQEQSDSKKFSKHGARECAKCGWRGDSRFCQNCGTEII
                     EEFCTNCGAQLTTSFCTSCGVARETLQGGSTSFAGTEAFDSEQAIHSGEVERGSENAA
                     QSAEDDAVAAFERACFEFGEQISGTWHWRYTTSLGTPMLCLLTIRVFGGEADADAFFL
                     TIGDETQFPSNCVRRVFYDLTKGSPIQVVTEAISYCSPAGISTDVVGMVMLSDGYTAW
                     ENEKVGAANEYEYDDELDILQCTGAVYERMPRSRVTTIMQGLF"
BASE COUNT          563 a          668 c          581 g          606 t
ORIGIN      
        1 ggtaagatga tctgtaaatg gtcacgatcg acgcgcaggg cctgaggcgg tccaaacgca
       61 aagtaggttg gagatccgca agcagttaca agatcatgcg tgtagattct tggctcttcc
      121 cctccgccaa ttgatttggc tttgcggaag tcttcgcagg cgccgctctt attctcggct
      181 aggaggcgca aagatccgcg agcggcaaga gcatcgggat actcatcttt cgcccaggct
      241 accgcggcgt cacattccgc tagagcttct tctaaccacc gatcatatcc gacttctcgg
      301 tggagatcgt cccattggaa ctgtttgccg tgcttaatcg ctcgcctacc caatgggtag
      361 tttaccgcgc aaagttcagc ataataacgc cacgtgttct ttccctccgg atccagattg
      421 atggctatcc ggaaagctga ttttgctgaa ccgtagtagc ctgaatcctc cattccgaca
      481 ccctccttga aattttgtgc tgcacattgc accgggcttt gatccctttt ctggcaaatc
      541 tcttgggcat tttgcatttc ttgcgagacg catgcgatgc tggccatgat ggcccatgca
      601 ctcagaagat gaatttttgc cgctcgggag atagatttca ttccaccgtg gtgcgaacgg
      661 ttgacggctt cgtcaatttg atatcggtag tctcgaaggg tcggcggcaa catcttaagt
      721 ctgcagaaat tcagccggaa aatactcacc gtcaaaatca ttgcccgttg cgtccacggt
      781 cacggcgccg tccggatggg tataagaaat gcttgcgacg aatgatgttc cgatgatctg
      841 attttcacac accgcgatcg ccagcctgtc aatggaaaac atatcgctgc ggcccaatga
      901 gaactctgcc gcggaaggtg aatcggagcg atatccgaat atatagagaa tccgttgacc
      961 ggcgcgcgac gcgctccaat cttccgcaga aactgttcta cggttcgcct ggcgcaaatg
     1021 cgtgaaggga atttcaatgc gatctgacgc gtgggtcacg atgatcgaaa gatcttcatc
     1081 cgcgatgaaa gcgtttccgc cggacccaaa atcgtctgtc acggcagcgg cactgaaaag
     1141 atcccaattg tcatcttcga gcgctgcttg gactgactcc tcctcactcg ggccaccaaa
     1201 gaaagtttcg atggcttcat catgctcttc gcagtaatct cgtattttct ggcattctag
     1261 tccagcaaac tcaagggccc agaacgcaat catgttgccc gttagcgtga cctgaatttc
     1321 gttcatcaga acaacccctg cataatcgta gtaactctgg atcttggcat gcgctcgtat
     1381 accgcgccgg tgcattgcag gatatcaagt tcatcatcgt attcgtactc gttagctgcc
     1441 ccgacttttt cgttttccca tgcggtgtag ccgtcagata acattaccat accgaccaca
     1501 tcggttgaaa tgcctgccgg ggagcaatac gagatcgctt ctgtcacgac ctggatgggt
     1561 gaacctttcg tcaggtcgta aaacacacgc cgcacacaat tggaagggaa ctgtgtttcg
     1621 tccccgatag taaggaagaa ggcgtcagca tcagcttccc cgccaaaaac gcgaatggtg
     1681 agcaaacaga gcatgggcgt cccaagactc gtcgtatacc gccaatgcca ggtaccggaa
     1741 atctgttctc cgaactcaaa gcatgcgcgc tcaaaggcag caacagcatc gtcttcagcg
     1801 gattgagccg cgttctcact ccctcgctcc acttcacccg aatgaattgc ctgttctgag
     1861 tcaaaagctt cagtgcctgc aaacgaagtg ctgccccctt gaagggtttc gcgcgctaca
     1921 ccgcaactag tgcagaatga agtggtcagt tgcgcaccac aatttgtgca gaattcttca
     1981 attatttcag tcccgcaatt ctgacagaac cgtgaatcac cgcgccaacc acatttcgca
     2041 cattctcttg ctccatgctt cgaaaacttc ttcgaatccg attgctcttg aaaagcttca
     2101 gagttttcca tggatcaccc gcctaacctg agaatcttct ttcgcaattg cccaaggttc
     2161 ttgttgctaa tccttgagag gtgggtgtgt tcaatcacgt ttcttccggt cggattctgg
     2221 atacccagac tttcgcggat acgagaatcc cgcaagagtg cgctaccgag gaagactatt
     2281 ttctcagacc cttggagcca gatttgcaat tgcttcaaat gtggcgcatt ttgtttacta
     2341 gagactgccc gcatgagggt cgcggccccc caagaacttc cgcgtgttgt gtgcttcgct
     2401 aaaagctcca caaacgcc
//
LOCUS       DAORFF010000590         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_32389_length_2413_cov_3.059372, whole genome shotgun sequence.
ACCESSION   DAORFF010000590 DAORFF010000000
VERSION     DAORFF010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32389_length_2413_cov_3.059372"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PKK76_17845"
     CDS             <1..565
                     /locus_tag="PKK76_17845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL70396.1"
                     /translation="LLIRLRRQIANLELFARGFLDLEKGAQPASEVKTLDVGALWLSL
                     LDAHAEEMKKKSLRLKIAGDMTGQMVRCAESDLVRILENLLSNALKFSLPGGCIWTSV
                     RGEESDIVLDLEDCGPGFTASTEMDPFDLARRNLGRTGTGYGLGLAAALDLAESMNGK
                     LSLANPLHGAGARFLLVLPGAELSLDT"
     gene            562..789
                     /locus_tag="PKK76_17850"
     CDS             562..789
                     /locus_tag="PKK76_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70397.1"
                     /translation="MTGIHLRNTTGKRSGSFIRYEYGEDLFGYVYLDVIRGRRHRAQK
                     VWTALFTSKIDFLITLDMDLERKENLNYVHA"
     gene            854..>2413
                     /locus_tag="PKK76_17855"
     CDS             854..>2413
                     /locus_tag="PKK76_17855"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70398.1"
                     /translation="MENSVFVLGSGPLGLILSSNLSEKASSVTLCWHDSAEAARMRRD
                     GGTRLLNQDFFFPDNVEIADRTAYLPDDCILIVAISSRELEETMEAMLASFKDRSGLI
                     VLFTKGLPSYTARKKYNARTFCEYMEKFSHDRGIHQLKFAAVNGPSLLSELYHGSYSF
                     LNVGCADTDAGKRIAELLSNDRIQCGYTSDTIGVEMGGVLKNPIAIASGIATNLAECG
                     GNFAGELIQHGFQEMLSLSLAIGARQETLIGRSGIADLMTTCLSKESRNRAYGEDFIK
                     RLQSGSEEHGVMDRIETFLNPARVIQKDAAQGQDVVEGAYAIASIVEIAAELKVEVPL
                     YNALYEILSRRSPPNALISAITGAPVRASEPQVLHRRQGLHLAAGTDFVEVLSSRVFH
                     SVQVTKGMQSRIKKQATQVLTQLDKRLQKAKKVKNRMDLQKIPHERDLWLSFSSSIGE
                     SESRALRHLIQFYAEEIADSYTPGMRETLIRLLAPVRFAASGFKLGSAMPKIGGNTEE
                     LKNLAAHYNVMY"
BASE COUNT          592 a          586 c          641 g          594 t
ORIGIN      
        1 actcttgatt cgtctcaggc gacagattgc gaatctagaa ttgttcgcgc gcggctttct
       61 agatctggag aaaggggccc agccggcgtc agaagtgaag acactggatg tgggggctct
      121 gtggctctcg ctcctggatg cacatgcgga agagatgaag aagaagagtc ttcgattgaa
      181 aatcgcggga gatatgacag gacaaatggt tcgatgcgca gaaagcgatc tggtccgaat
      241 tcttgaaaac cttctgtcca atgctttgaa gttctctttg ccgggtggtt gcatctggac
      301 ttccgtgagg ggtgaggaat cggacattgt gctggatttg gaggattgtg ggccaggatt
      361 cactgccagt accgaaatgg atccttttga tctggctcgc agaaatctgg gaagaacagg
      421 gacaggatac gggcttggcc tcgctgcagc tctcgacctg gcagaatcaa tgaatgggaa
      481 actttccctc gccaatcccc ttcacggcgc cggcgccaga tttttgcttg tacttccggg
      541 cgcggagttg tctctggaca catgaccggc atccaccttc gcaacacgac gggcaagaga
      601 tcaggttcct ttattcgcta cgaatacggg gaggatttgt ttggctacgt gtatctcgac
      661 gtgatccgcg gtcgtcgtca tcgtgctcaa aaggtttgga ctgcactgtt taccagcaaa
      721 atcgatttcc tgatcactct ggatatggac ctggaaagaa aagagaacct gaactacgtc
      781 cacgcctgac ctgctggatt tttcctaaca aatcgcactt gaaaaaaaat gacccgccat
      841 tcacggtcgg gtcgtggaaa acagtgtttt cgtacttggc tccgggcccc ttggtttgat
      901 cctttcttct aatctatctg agaaggcttc ttccgtcact ctatgctggc atgattctgc
      961 ggaagcggcg cgaatgcgcc gcgacggtgg cacgcgcttg ctcaatcagg atttcttttt
     1021 cccggacaat gtggagatag cggatcgaac tgcctatctt ccagacgatt gcattctgat
     1081 cgttgcgatc tcttctcgcg aactcgaaga aacgatggag gccatgcttg catctttcaa
     1141 agatcgctca ggtctgatcg ttctgtttac taaaggactt ccatcttaca ctgcacgaaa
     1201 aaaatacaac gcgcgcactt tctgtgaata catggaaaaa ttttcgcatg atcgcggcat
     1261 tcatcagctt aaatttgccg ccgtcaacgg acccagttta ctctccgaac tctatcatgg
     1321 atcctacagc tttctgaacg taggctgcgc cgacacagac gcggggaaac gaatcgcgga
     1381 gttgctttct aacgatcgca tccagtgcgg ttacacctct gatacaatcg gcgtggaaat
     1441 gggcggcgtg ctcaaaaatc caatcgcgat tgcatctggc attgcaacga acctggcgga
     1501 atgcggaggc aacttcgcag gcgagctcat acaacacggc ttccaggaaa tgctttccct
     1561 ttctctggca attggcgctc gccaggagac tctcatcgga agatcgggca ttgcagactt
     1621 aatgacaacg tgcttgagca aggaaagtcg caatcgggct tatggtgaag atttcatcaa
     1681 acgcctgcaa tctggttctg aagaacatgg ggtgatggat cgcattgaaa catttttgaa
     1741 tccagcgcgg gtgatccaga aggatgcggc tcaaggtcag gatgtagtag aaggcgccta
     1801 tgcgattgct tctattgtag agattgcagc tgagttaaaa gtagaggttc cgctttacaa
     1861 cgcgttatac gagattctgt cgcgcagaag tcccccgaac gcactcatat ccgcgattac
     1921 aggtgcgccc gttcgagcaa gtgagccgca ggttctgcat agaaggcagg gtttgcacct
     1981 ggccgccggg acagatttcg tagaggtact ctcttcgcgt gtttttcata gcgttcaggt
     2041 gaccaaagga atgcaatctc gcatcaagaa gcaagcgacg caagtactca ctcaactgga
     2101 caagagactt cagaaagcaa agaaagtgaa gaatcgtatg gatcttcaga agattccgca
     2161 cgaaagggat ttgtggctgt ctttctcttc ttcgataggt gaatcagaaa gccgcgctct
     2221 acgacatttg attcaattct acgcggaaga gatcgcagac agctacactc caggcatgcg
     2281 cgagactctc attcgtcttc ttgcccccgt gcgatttgct gcgagtggtt tcaagctggg
     2341 atctgccatg ccaaaaattg gtggaaacac ggaggagttg aagaacctcg cagcacacta
     2401 caacgtgatg tac
//
LOCUS       DAORFF010000591         2398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_32766_length_2398_cov_2.247119, whole genome shotgun sequence.
ACCESSION   DAORFF010000591 DAORFF010000000
VERSION     DAORFF010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32766_length_2398_cov_2.247119"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1789
                     /locus_tag="PKK76_17860"
     CDS             <1..1789
                     /locus_tag="PKK76_17860"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70399.1"
                     /translation="QGRKAAEEKNKLAIEKKYETSTQLELDLGAEIESLRDASRKLEE
                     GIAKREAEIRLNVEKEEANHEELRKSEEEQESLLARMRQVTDELVLELEAKHKDLEQH
                     EAQRRKLHDSFGALAASAAEKLNKLKTAVAAGKREEASELIAAIKLDDLKRVFDEAEA
                     LDARLRSVLFGKTGLLTQKEALDASMEALRARREGLQKENSALFEKRKADLTQNEKEK
                     NRRMEIELDIAAGDERRKSSSQGLEAIRVQIAEVQERISFLSQEVEEVGSACARLKEQ
                     EASVLKELGERKKSSDGQKTEVESILKKIEKKHKEIADLKEKYRRDREQLDRVLPAIG
                     EQERLVEHISDDIQELTENLYNDFQVEIPEVEAEFAGDSSKFREQESEFKRIRQEIQS
                     LGQFNALAIEELDRATESYNVLMDQRKDIEAAQENILTVLRETDQKSREIFLDNFRRI
                     ENNFQEVFQALFGGGKATLSLTNPEDPLQGGVEIMVQPPGKKNSSITLLSGGEQNMTA
                     IALMFATYLVRPSPFCFLDEIDAPLDENNVLRFLRMLGSFTNRSQFLVITHCKQTMAR
                     ASNIFGVTQEEAGVSRIVSVQLSEASRSG"
     gene            1789..>2398
                     /locus_tag="PKK76_17865"
     CDS             1789..>2398
                     /locus_tag="PKK76_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70400.1"
                     /translation="MRGASFSFLVATAICTFASSLFALPPVVDRPPVRANESKIVLLP
                     FSNESRETTLDYLRSGIPESLAGYFSSVGYIEDSRAIVYVMRTQGAPGQGVLRETTRD
                     NLFADVDTVKLTPEIYQAVESGDLAGVARRLSARYVVRGSFRADPEEKVTEARRLHVD
                     AEIYDGRTGQKKRNSIDTKVDSLHEDLAGMASDLRAVIRVPQK"
BASE COUNT          689 a          555 c          648 g          506 t
ORIGIN      
        1 acagggaaga aaagccgcag aagagaaaaa caaactcgcc atagaaaaga aatacgagac
       61 atccacgcag ctagaactcg acctgggtgc tgaaatcgaa agccttcgcg acgcttcgcg
      121 aaagttagaa gaaggaattg caaagagaga agctgaaatc cggctgaacg tagaaaaaga
      181 agaagcaaat cacgaagaac ttcgcaaaag cgaagaggaa caggaaagtc ttctcgctcg
      241 catgcgtcag gttacagacg agctggttct ggaactggag gcaaagcaca aggacctgga
      301 acagcatgag gcgcagagaa gaaagctaca cgattcattt ggcgcgctgg cagcatccgc
      361 tgcggaaaaa ctaaacaaac tcaagaccgc ggttgcggca ggtaagagag aagaagcatc
      421 cgaactcatc gccgcgatca aactggacga cctcaaacga gtgtttgacg aagcagaagc
      481 actcgatgcg agattgagaa gcgttctgtt tggaaagaca ggactcttga cccagaaaga
      541 agcactcgat gcgtccatgg aggcccttcg cgctagaaga gaagggttgc agaaagaaaa
      601 ctccgctctc tttgaaaaga gaaaggcaga cctcactcag aacgaaaaag aaaagaatcg
      661 tcgcatggag attgaactcg acatcgctgc gggagatgag agaagaaaat catcttcgca
      721 gggtcttgag gcaattcgcg ttcagatcgc agaagtacag gaacgcatat cctttctatc
      781 ccaggaagtg gaggaagtgg gttctgcgtg cgcgcgactg aaagaacagg aagcctccgt
      841 tctcaaagaa ctcggagaaa gaaagaaatc aagcgatggc cagaagacag aagtagaatc
      901 tatactaaag aaaattgaga aaaaacataa agaaatcgca gatctcaaag agaaatacag
      961 gcgtgaccga gagcagcttg atcgagttct tcctgccatc ggggaacagg aacgcctggt
     1021 agaacacatt tcagatgata tccaggaact gacggagaat ctgtacaatg actttcaggt
     1081 ggaaataccg gaagtggagg cggaatttgc gggggacagc tcgaagtttc gcgaacagga
     1141 atctgaattc aaaagaattc gtcaggaaat ccaatccctg ggccagttca atgctcttgc
     1201 gattgaagaa ctggaccggg ctactgaatc ctacaacgtt cttatggatc agagaaagga
     1261 catagaggct gctcaggaaa atattctcac tgtgcttcgc gaaacggatc agaagtcccg
     1321 ggagatattc ctggataact tccgtcggat agagaataac ttccaggaag ttttccaggc
     1381 tctcttcgga ggcgggaagg ccactctcag tctcaccaat ccggaggacc cccttcaggg
     1441 tggcgtggag atcatggtgc agcctccagg taaaaaaaat tcaagcatca ctctgttgtc
     1501 cggtggtgag cagaacatga cggctatcgc gctcatgttt gctacgtatc tggttcgtcc
     1561 ttctcctttc tgttttctcg atgagatcga tgctcctctc gacgagaaca acgtattgcg
     1621 tttccttcgc atgctcggca gttttactaa tcgttcccaa ttccttgtga tcactcactg
     1681 caagcagacg atggctcgtg cttccaatat cttcggggtg acacaggagg aggcgggagt
     1741 gagccgcatt gtttccgtgc aactatcaga agcgtcccgg agtggctgat gcgcggcgcc
     1801 tccttctctt ttctcgttgc cactgcaatt tgtacctttg cttccagtct tttcgcgttg
     1861 cccccggtag tcgatcgccc tccagtgcgc gccaacgaat cgaagattgt gcttctaccg
     1921 ttttccaatg aatcacgcga aaccactctc gattaccttc gctctggaat tccggaaagt
     1981 ctcgcgggat atttttcgtc tgtaggctat atagaagact ctagagctat agtctacgtc
     2041 atgcgaacgc agggtgctcc aggccaggga gtgcttcgtg aaaccacgcg agacaatttg
     2101 ttcgctgatg tagacacagt gaagttgacg cctgagattt accaggcggt ggagtccggt
     2161 gatttggccg gtgtggcgcg gaggctttct gcgcgctacg tcgttcgtgg tagctttcgc
     2221 gcagatccag aagagaaagt gacggaagca agaagactgc acgtggacgc ggaaatctac
     2281 gacggtcgca ctggacaaaa gaaacgcaat tctatagata caaaagtgga cagtcttcat
     2341 gaggacctgg ccgggatggc ctctgatctt cgtgctgtga ttcgcgtgcc acagaaat
//
LOCUS       DAORFF010000592         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_32866_length_2393_cov_4.910180, whole genome shotgun sequence.
ACCESSION   DAORFF010000592 DAORFF010000000
VERSION     DAORFF010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32866_length_2393_cov_4.910180"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..896)
                     /locus_tag="PKK76_17870"
     CDS             complement(81..896)
                     /locus_tag="PKK76_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70401.1"
                     /translation="MILSKILCSICLVLCVTVASLNAQSVPGDTNRTTFWAFEATIAR
                     GFFLHSPGAADVLGYDSSDRKPVGDYTMRYGTSFEVLRNSYAYKTAPQTDFDSSDEFR
                     LSASRKILPWLHLGLDAEHVSYRIANMHMNVRQYTNPYNQFNILTFGVISFPTLRDAA
                     VNGELLDPILLHNGWPINTITAGTHVAFHFSEGNIDPFLKVTVAVGKDLYRPLSTFEY
                     GASGGLRVFISERVFVTTEAGIRLVQFNGHAGGRTVSALLREPSFRAGAGFTR"
     gene            complement(893..1741)
                     /locus_tag="PKK76_17875"
     CDS             complement(893..1741)
                     /locus_tag="PKK76_17875"
                     /inference="COORDINATES: protein motif:HMM:NF027222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HNL70402.1"
                     /translation="MRPVFMHPVFLLITWFILSCRPDYTKSQSDAAIQSLLFAGLSSN
                     VVTCAQPYTGQASVDTIIDQIKSTYGVQPCVNLGPLPLFTLGTVTSSDYANLQTALTT
                     LQTEFARYPIELVLRSRLRMISFPTSIGVPYGGTAGGFAPTELDFIMVRLQVSNIPFL
                     KATIHHEFFHHLDEEKNGPRYYTDPTWRSYNYTYYSYGCNRSNTSAGVISPPAAGFLN
                     TYSTCEVVEDKAEVYSNQFILPNKTQIQTLIANGDTAVAQKLDYVQRVAANFSSSINQ
                     TFWNNL"
     gene            complement(1773..>2393)
                     /locus_tag="PKK76_17880"
     CDS             complement(1773..>2393)
                     /locus_tag="PKK76_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70403.1"
                     /translation="VEDLNYWKTNSVFYVNDSGGVFTGAGWTEKSAKQILWKAATLTM
                     AEFTAVVPADHRLPYSGGLQEFKKQSVPIYLSKKSRTLMLEQWKDLNLDMSSFGSWKE
                     IPAAEPDHIFDGSMEFLGGSVIVREIDAGQRSSAIAAYFPAERILFLGSALSDPPYFY
                     TYDRKALRSSITELMKLPFEIAISGHGKAIRNRAFVEDQLRQLNAD"
BASE COUNT          602 a          555 c          636 g          600 t
ORIGIN      
        1 gacgcatgtt cctcccacac tctccagggc catgcgaatg ccgccaattc cggcgaacaa
       61 atcaatgaat cggaaagact tcaacgagtg aatccggcgc ccgcacgaaa agatggttct
      121 ctcaaaaggg ccgaaactgt acgcccgcca gcgtgtccat tgaattggac cagtcgaatg
      181 cctgcctccg ttgtaacgaa tacgcgctcg ctgatgaaga cgcgcaatcc gccggatgca
      241 ccgtattcaa atgtgctgag tggtcgatat agatctttgc ccactgcaac cgttactttt
      301 agaaatggat ctatgtttcc ttctgagaag tgaaatgcaa catgagttcc ggctgtgatt
      361 gtgttgatag gccatccgtt gtgaagtaga atcgggtcga gcaactcacc gtttacagca
      421 gcatcgcgaa gagtggggaa cgaaatgact ccgaaggtta gtatgttaaa ctgattgtat
      481 ggatttgtat actgtctaac attcatatgc atgtttgcaa ttcgatagct tacatgctct
      541 gcatccagac ccaggtgaag ccacgggaga atctttctgg aagcagaaag tctaaattca
      601 tcggaggaat cgaaatctgt ctgaggagct gttttgtagg cgtagctgtt tcgcaatact
      661 tcaaaagaag taccgtatct catggtgtaa tcgcccactg gctttcggtc agatgaatcg
      721 tagccaagca cgtcagctgc ccctggcgaa tgaaggaaga agcccctggc gatggttgct
      781 tcgaatgccc agaatgttgt tcggttcgtg tcgcccggca ccgattgcgc gttcagggac
      841 gctacagtca cacaaagtac cagacaaatt gaacaaagga tcttactgag gatcataggt
      901 tattccagaa agtttggttg atgctggagc tgaaattcgc cgcgaccctc tggacgtagt
      961 ctagcttctg tgcgaccgct gtatctccat tcgcgatgag tgtttggatt tgcgtcttgt
     1021 ttggcaggat gaattgattg gaatagactt ctgccttgtc ttccaccact tcacatgtgg
     1081 agtacgtatt gagaaaaccg gcggctggag gagagatcac tcctgcagat gtattggatc
     1141 gattgcagcc gtaggaatag taagtataat tataggatct ccaggttgga tccgtatagt
     1201 agcgagggcc atttttctct tcatccaggt ggtgaaagaa ttcatgatgg atggttgctt
     1261 tgaggaaggg gatgttcgac acctgaagcc gaaccatgat gaagtccagt tcggtgggcg
     1321 caaatccgcc tgcagttcct ccgtacggaa ctccaataga agttggaaaa gaaatcattc
     1381 tgaggcgaga gcgaagcacg agttcaatgg gatatcgtgc gaattcagtc tgcaatgtgg
     1441 tgagtgctgt ttgtagattt gcgtagtcgc ttgaagtaac ggtacccagc gtgaaaagag
     1501 gaagcgggcc caggttgacg catggctgta ctccgtacgt agatttgatc tggtcgatga
     1561 tcgtatctac ggatgcctgg cctgtatatg gttgtgcgca tgtgacaaca ttggagctga
     1621 gacctgcgaa gagcaaagac tgaatggctg cgtctgattg actcttcgtg taatccgggc
     1681 ggcagctcag gatgaaccag gtgatcaata ggaagactgg atgcatgaag acggggcgca
     1741 tagccaacga tcgcatgcgc tgcgatacac ggtcaatccg cattcaattg ccgcagctga
     1801 tcttccacga aagctcgatt gcggatggcc ttgccgtgtc cgctgatggc gatttcaaat
     1861 ggaagcttca ttagttctgt gatcgaggac cgaagcgcct tacgatcgta cgtgtaaaag
     1921 taaggcggat cggaaagagc gctgcccaaa aagaggatgc gctctgccgg aaagtaggct
     1981 gcaatcgcag aagatctctg tcctgcatct atttctcgca caatcacaga gccgcccaga
     2041 aactccatcg aaccatcaaa gatgtgatcc ggctccgcgg ctgggatttc tttccaggac
     2101 ccaaagctgc tcatatccag gttcaagtcc ttccattgct ccagcatcaa cgtcctggat
     2161 tttttggaaa gatagatggg gacagactgc ttcttgaatt cttgcagccc accgctgtaa
     2221 ggcaatctgt ggtctgctgg aacaacggcg gtgaattcag ccatagtcag tgtggcggcc
     2281 ttccataaaa tctgcttcgc cgatttttca gtccatccgg cgcccgtgaa gactccaccg
     2341 gaatcattca catagaagac tgaatttgtt ttccagtaat tcaagtcttc cac
//
LOCUS       DAORFF010000593         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_32876_length_2393_cov_3.180924, whole genome shotgun sequence.
ACCESSION   DAORFF010000593 DAORFF010000000
VERSION     DAORFF010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32876_length_2393_cov_3.180924"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1818
                     /locus_tag="PKK76_17885"
     CDS             <1..1818
                     /locus_tag="PKK76_17885"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNL70404.1"
                     /translation="VTEPDQVIWQSIPALTRLGAKTAVIRIPVMGGAGRTLYIPASTF
                     AMRVYCDSKIIYSFSDETQFTPRHFRGWAYHVVDLDPCTQGILYFHVYSALNVRFVIP
                     HSGTTEDVIRFLILNAIGPFIVVVICVLLGCGFFLLFLIQTDRLSGSLSLFLLCTGIW
                     LFGLNPLSKLLFHDHPLWASLAVTCLYASPAGFALFAERIMPGSILFRNGLGAAVFVL
                     FAAAALFLDLTGVIPLYRSILPFNVAVLCVALLTAVQLIRGLRHGRHEARILGIGVAC
                     LIVFAVHDMLLAFSTFAGVIDLRVSRLHYGMLGFILSMVGVAANQWKQMQSDIHRHSR
                     ELERRVNERTAHLESALAAIRANDVRVREEIAIASSIQRQLLLQLPIEMPGLRISGCY
                     LPMHDVSGDFYDIYYNENGSVIFFLADAVGHGVPAALIMSMLKAIFLESAPLFDDPSW
                     ILAHMNNRLEKHLARTGNYATGTVLFIPDKGPVLYSNAANRPAVILRENGTPEWLDGN
                     GLILGAFPQQPEDYTLQTVELAPGDRILLFTDGITEQTFENDAFGEERLFDFLHGKSH
                     LESDALTNAVLGELARFAHKKDFDDDVTICVMERLGKKK"
     gene            complement(1833..>2393)
                     /locus_tag="PKK76_17890"
     CDS             complement(1833..>2393)
                     /locus_tag="PKK76_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70405.1"
                     /translation="LVEKIAGTPPNHANEALLDRLTEMMDEREVGLRLLTMIQNSSEV
                     EGPEKIGKAISEVHDIAREEGRRKGGRFDRKGGGRFGRNRRDRASAHSDRQERDREGR
                     GAGIYSASASHSAKSSHGKALNERSSHDKASHEKSAHARSSHGSSGSRKNEGKAKSGK
                     VFRKAGPGFGNHARKDRKKKGPKKKH"
BASE COUNT          505 a          640 c          578 g          670 t
ORIGIN      
        1 gtcacagaac cagaccaggt gatctggcag agcattccag cgttgaccag actcggcgcg
       61 aaaaccgcag tcattcgcat ccctgtgatg ggaggagcgg gacgaaccct gtatattcca
      121 gcaagtacgt tcgcgatgcg cgtctactgt gactccaaaa taatctactc tttttcagac
      181 gaaacgcaat tcactccgcg gcatttcaga ggttgggcat atcatgtagt cgatctcgat
      241 ccatgcactc agggcattct ctacttccat gtgtattctg cgttgaacgt tcgctttgta
      301 attcctcaca gcggaaccac ggaagatgtg attcgctttc tgattttgaa tgcaatcgga
      361 cctttcatcg ttgttgtgat ttgcgttttg cttggatgcg gattcttcct gttgtttctc
      421 atccagacag atcgattgtc cggttctctc agcctgtttc tattgtgcac tggcatctgg
      481 ctgtttggtt taaacccact gtccaaactg ctgtttcacg atcatccact ctgggcaagc
      541 cttgctgtca cctgtctcta tgcatctccc gcgggctttg ctctcttcgc agagcgaatc
      601 atgccaggat ccattctgtt tcgaaatgga ctgggggcgg ccgtcttcgt tctgtttgcg
      661 gcagcggcac tgtttttgga cctcaccgga gtgatccctc tctacagaag cattctcccc
      721 ttcaacgttg cggttctgtg cgttgctcta ctcactgcag tgcagctgat tcgcgggctg
      781 aggcatggcc gccatgaagc caggatcctt ggcattggag tggcttgctt gattgtattt
      841 gctgtgcacg acatgctcct tgcattttct acattcgccg gagtgattga tcttcgagtg
      901 agcagacttc actacgggat gcttggattc attctgtcta tggtaggagt ggccgccaac
      961 cagtggaagc aaatgcaaag cgacatccac agacattcca gagagctaga gcgaagagtc
     1021 aatgaacgaa ctgcacatct ggaaagcgca ctcgctgcca ttcgagccaa tgacgtgcgc
     1081 gtgcgagaag aaattgcaat tgcgtcgagc atccagcgcc agcttctgtt gcagcttccc
     1141 attgaaatgc caggccttcg catatcgggc tgttatctgc ccatgcacga cgtgagcgga
     1201 gatttctacg acatctacta caacgaaaac ggaagcgtca tcttctttct ggcagacgct
     1261 gtaggtcacg gggttcccgc agcactcatc atgtccatgc ttaaggcaat ctttcttgaa
     1321 tcggcaccgc tctttgatga tccttcgtgg attcttgctc acatgaacaa caggctggaa
     1381 aagcacctcg cacggacagg caactatgcc acgggtacag ttttgtttat cccggataaa
     1441 ggacctgttc tgtattctaa cgcggccaat cgacctgcag tgatccttcg cgaaaacggg
     1501 acaccggaat ggctcgatgg caatggattg attcttggcg cctttccgca gcaaccggaa
     1561 gattatacat tgcagactgt ggaactggca cccggagacc gcatcttact ttttacagat
     1621 ggaatcacag agcaaacctt cgaaaacgat gcatttggag aagaaagact ttttgatttc
     1681 ctgcacggga agtctcacct ggaatccgat gctctcacaa atgcagttct gggggaactc
     1741 gcgcggttcg ctcacaagaa agactttgat gatgatgtga ccatctgcgt gatggaacgc
     1801 ctcgggaaaa agaaatagga tctcacccga tctcagtgtt tcttttttgg acccttcttc
     1861 tttctatctt ttcgcgcgtg gtttccaaaa ccaggtcccg ccttacgaaa caccttgccg
     1921 ctctttgcct tgccttcgtt ttttctggat ccagaagaac cgtgcgaaga cctggcatgc
     1981 gcagactttt cgtgcgaagc tttgtcatgc gaggaccttt cattcaatgc tttgccgtgc
     2041 gacgatttcg cggaatggct tgcgcttgct gaataaatgc cggcccctct tccctcgcga
     2101 tccctttcct ggcgatctga atgagctgaa gctctgtctc gcctgtttct tccaaatctt
     2161 cctccgccct tgcgatcaaa tcgcccacct ttgcgtcttc cttcttcgcg cgcgatgtcg
     2221 tggacttctg aaattgcttt tcctattttt tccggccctt cgacttcgga tgaattttga
     2281 atcatggtga gaagtctcag gcctacttca cgctcatcca tcatttcagt gagccgatcg
     2341 agaagcgctt catttgcatg attgggtgga gtgcccgcga tcttctctac cag
//
LOCUS       DAORFF010000594         2392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_32904_length_2392_cov_3.271716, whole genome shotgun sequence.
ACCESSION   DAORFF010000594 DAORFF010000000
VERSION     DAORFF010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32904_length_2392_cov_3.271716"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PKK76_17895"
     CDS             <1..506
                     /locus_tag="PKK76_17895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNL70406.1"
                     /translation="IENIRRLSSQVENVLLSGEMENASFEMLSQSCDCVLVLHTYVAR
                     LPEKDSIHVESPHSLKVSLPQHLLERLYDILIQNALVHARGAVTVSVRSELSEVKITV
                     ADNGPGIPESELENIFKPFYRLSEARGTGLGLYLARQIVTIGHGTLTARNNIPRGALL
                     EVRLPPG"
     gene            514..1185
                     /locus_tag="PKK76_17900"
     CDS             514..1185
                     /locus_tag="PKK76_17900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNL70407.1"
                     /translation="MILLVEDEPAIRSGIRINLEAEGFSVLEFPDADSAAGEIARTKP
                     LISLGILDIMMPGKLNGLDLCTKLREMGQSFPVIFLTARGRLEDKLAGFKSGADDYVT
                     KPFELEELIARIYARLGKKAKTRIGKFQLDFESATAESDSGEIVRFNDKEIQMLSVLA
                     QNRGKPVRRDEILDAVWGADFPTNRTIDNFIVKFRKIFEDDPAQPVHFITRHGTGYEL
                     SQENV"
     gene            1182..2222
                     /gene="hemE"
                     /locus_tag="PKK76_17905"
     CDS             1182..2222
                     /gene="hemE"
                     /locus_tag="PKK76_17905"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNL70408.1"
                     /translation="MKPALIRALEGEMLDRIPFWYMRQAGRYLPEYNELRRGRTFIEV
                     MESADLSVEISLQPYRRFKMDGVIMFSDILTPVRGAGIPLHFEEKKGPVLEKTVQSES
                     DIDMFRAFQAEKACPYVKEILTRLRAHLASEEDRPALLGFAGSPFTLASYLIEGGGTQ
                     KFEKTKECIFRRTELFNSLCDALTDLTIQYLRMQIDAGAEAVQIFDSWGGILSPAHYK
                     EFSAPYTARIISALRADGKAPVILFVGNGSHLVPLMAEQKPDALSLDWRISADQVRRE
                     IPDGIAIQGNLDPLVLYGTAERTGNETRKVLAAFGNRKGYVFNLGHGIHPATPIENVQ
                     AMIDSIRSFKRS"
BASE COUNT          623 a          607 c          597 g          565 t
ORIGIN      
        1 gaatcgaaaa tatccgaagg ctttcttctc aggtagaaaa tgtgcttttg tcgggggaaa
       61 tggaaaacgc gtccttcgaa atgctctcgc aatcctgcga ttgtgtgctg gtattgcaca
      121 cctacgtcgc ccggctgcca gaaaaggact ccatccacgt tgaatctcct cactcattga
      181 aagtcagtct gcctcagcat ttgcttgagc gactgtacga tattctcatc caaaacgcgc
      241 tcgtccacgc aagaggcgct gtaaccgttt cagtgcgaag cgaattatct gaagtgaaga
      301 tcacagtggc agataacggg ccggggattc ctgaatctga gctggaaaac atattcaagc
      361 ccttttatcg tttgtctgaa gccaggggaa ccggtcttgg cctctacctc gcccgtcaaa
      421 ttgttaccat tggacacggc actctcaccg cgcgaaataa cattccacgg ggcgctctcc
      481 ttgaagtgag gctgccccct ggctaaaaag caattgattc tgctcgtaga agacgagccc
      541 gctatccgct ccggcattcg catcaacctt gaggcggagg gattctctgt tctggaattt
      601 ccggatgcag attctgccgc aggcgaaatc gccagaacaa aacctttgat ctccctgggc
      661 attctggaca tcatgatgcc gggaaaactc aatggacttg atttgtgcac gaagttacga
      721 gaaatggggc agagctttcc agtcatattc ctcacggcgc gcggcagact ggaagacaaa
      781 ctggcaggct tcaaatccgg cgcagatgac tacgtaacca agccttttga actggaagaa
      841 ctgatcgcac gcatttacgc acgactcggc aagaaagcaa agacccgcat aggtaaattc
      901 caactcgatt tcgaatctgc aactgctgag tccgactcgg gggagattgt gcgattcaac
      961 gacaaagaaa ttcagatgct ctctgttctg gcacagaatc gtggaaaacc tgttcgccgc
     1021 gacgaaatac tcgacgcggt ctggggcgcg gacttcccaa caaataggac cattgataat
     1081 ttcattgtta aatttcgtaa aatatttgaa gatgatccag ctcaaccggt tcatttcatc
     1141 acacgccatg gaaccggcta cgagctgagt caggagaacg tatgaagcca gcattgattc
     1201 gcgccctgga aggtgagatg ctcgatcgca ttccgttctg gtatatgaga caggcgggaa
     1261 gataccttcc agaatacaat gagcttcgcc gcggcagaac attcatcgag gtaatggaaa
     1321 gcgcagatct atccgtagaa atcagtctgc aaccatacag acgattcaaa atggatggag
     1381 tcatcatgtt cagcgacata ctgactcctg tacgcggggc cgggattccg ctgcatttcg
     1441 aagaaaaaaa gggaccagtc ctcgaaaaga cagtccagag tgaaagcgac atcgatatgt
     1501 ttcgcgcatt ccaggcagaa aaggcatgcc cgtatgtaaa agaaatcctc accaggcttc
     1561 gagctcatct tgcatcggaa gaggatcggc cggcgcttct gggtttcgcg ggtagtccat
     1621 ttacgcttgc aagctatcta atcgaaggcg gcggaacgca gaaatttgag aaaacaaaag
     1681 aatgtatctt tcgcaggact gaactcttca attctctttg cgatgcactt acagatttga
     1741 cgattcaata tcttcgcatg caaattgacg cgggtgcaga agcggtgcag atcttcgata
     1801 gctggggagg cattctttcc cccgcgcact acaaagaatt ctcagccccc tatacagccc
     1861 gtatcatttc cgctcttcgc gcagatggaa aagctccggt cattttgttt gttggaaatg
     1921 gatcccacct tgttccactc atggcggaac aaaaaccaga tgcactttct ctggactggc
     1981 gcattagcgc agatcaagtt cgccgcgaaa taccggatgg cattgcaatt cagggaaacc
     2041 tggatccgct ggttttatac ggaaccgcag aaaggacggg aaacgaaacc aggaaggttc
     2101 tggccgcatt tggaaatcgg aaaggatatg tgtttaatct gggacacgga attcaccccg
     2161 ccactcccat tgaaaatgtt caggccatga ttgacagcat ccgatcattc aaacgcagct
     2221 agtcggttct atttcttgag gccaggattc gcgccgccag cggaaggtgt tcccgacgct
     2281 tgctgcgctg cctgctgcgc gagcaatttc tttctctgtt ccacggcggc ctgcaccttc
     2341 ttctcaagct gagaaacgtt gtcgaggtaa cgcttcattt cgttttcgcg tg
//
LOCUS       DAORFF010000595         2391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_32929_length_2391_cov_3.121147, whole genome shotgun sequence.
ACCESSION   DAORFF010000595 DAORFF010000000
VERSION     DAORFF010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32929_length_2391_cov_3.121147"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKK76_17910"
     CDS             complement(<1..238)
                     /locus_tag="PKK76_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70409.1"
                     /translation="MVRDYIKQLRVYQWTKSGFVVMPFVFSDYLPVVVSNPFSAGALE
                     LLFKLAAAFFAFSTLASSVYVFNDYRDRHQDALDS"
     gene            complement(274..1344)
                     /locus_tag="PKK76_17915"
     CDS             complement(274..1344)
                     /locus_tag="PKK76_17915"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HNL70410.1"
                     /translation="MDIFEATTAYESWLRTVTEVDSEALQVKHKAMCDGAFRFLRAAY
                     YDWIALWQSMPAEIKAAPVCPSIGDIHIENFGTWRDAEGRLIWGINDYDEATELPYTN
                     DLVRLATSVLLAIEDDRLEMKPRESCEAILSGYTRGLERGAKVFVLAEDNDWLRDIAR
                     RQIKDPGKFFRSMESLPDAAPPARVIEILKGILPEPFRVKKRIAGLGSLGRQRYAAIA
                     EVAGSRIVREVKANCPPSQNAFGSVAESLRARILENRPTSPDPFLLDASDYTVRRLAP
                     DCTKIEISLIGKTSDQIRLLASMGREISNVHCMRVESGLSAADSISSHLKSAPDRWLY
                     HAACTMADRIASRQKTWRRKMK"
     gene            1391..2305
                     /locus_tag="PKK76_17920"
     CDS             1391..2305
                     /locus_tag="PKK76_17920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNL70411.1"
                     /translation="MHRYSSNFFDAACIVPVRARPELLAQAVISILKGSTLPREIILV
                     LDGTAEERAADQNGVKQITHSAEAASVRLRVLETPAVGPAAARNTAVRATEMPWLTFL
                     DSDDLWETRKLEMQSTYMRKRPHLLACQTGESWMKNDKPVHRPRKLTPHTGRFLRDAV
                     HSCLLSLSAFMIRRDAFLDAGMFDPDFALCEDFDFSLRFLRSHAIGYLEEDLVEKRSG
                     SWKQLSQTPAIDAYRVRALCKLLGDHGRAGEGPGSIAERERSEAIRSLTHKVAILEKG
                     QEKFGQSAFVQNAIREGRAVLSLSSDDR"
BASE COUNT          559 a          604 c          627 g          601 t
ORIGIN      
        1 gactgtccag ggcatcctgg tgcctgtctc tgtaatcatt gaatacatac acagaacttg
       61 cgagagtaga gaatgcaaag aatgccgctg ccagtttgaa cagcagctcc agggcccctg
      121 cgctaaaagg attgctgact acgactggaa ggtagtcaga aaaaacaaac ggcatcacaa
      181 caaagccgga cttggtccac tggtagacgc gcagctgctt gatgtagtcc cggaccatac
      241 gcgatcgtcc gagcctatcg tcgcggggtc aactcacttc atttttctgc gccaggtttt
      301 ttgtctcgag gcgatgcggt cggccatggt gcatgcagca tggtagagcc accgatcagg
      361 agcacttttt agatggctgg agatcgagtc cgccgcagac aggccagact ctacgcgcat
      421 acaatgcacg tttgagattt cgcggcccat ggacgcaagg aggcggatct gatcggacgt
      481 ttttccaatc aatgaaattt ctatctttgt gcagtcaggc gcaagtcttc ggaccgtgta
      541 atcgcttgcg tccaataaaa aaggatccgg acttgtggga cgattttcga ggatccgggc
      601 gcgcagactt tctgcgacgc tgccgaatgc attttgagac ggcggacagt ttgcctttac
      661 ttcacgaaca attctcgatc cggctacttc cgcgatggcc gcataacgtt gtcttcccag
      721 acttccaaga cctgcgattc tttttttgac tctaaacggt tctggcagaa tgccttttag
      781 aatttctatc acacgcgcag gaggggccgc atccggaagg ctttccatgg aacgaaaaaa
      841 ctttccggga tctttgatct gtctgcgtgc aatgtctctg agccagtcat tgtcctctgc
      901 aagaacgaat accttcgcgc ctctttcgag ccctcgcgtg tacccggaaa gaatcgcttc
      961 gcacgattcg cgtggcttca tttccagcct gtcatcttca atggcaagga gcacactcgt
     1021 tgccaggcgt accaggtcat tggtgtaagg aagttctgtt gcttcgtcgt agtcattgat
     1081 tccccagatc aatcttcctt ctgcgtctct ccatgtgcca aagttctcaa tgtggatgtc
     1141 tccgatggac ggacagacag gcgcggcttt gatttcagcg ggcatcgatt gccagagtgc
     1201 gatccagtcg tagtaggcag ctcttaaaaa gcgaaatgct ccatcgcaca ttgctttgtg
     1261 tttgacctga agggcttctg aatccacttc tgtgacagtg cgcagccagc tttcgtaggc
     1321 ggtagtggcc tcaaaaatat ccatagctgg ttgtggtttg ccagggccgc ctgctcaagc
     1381 ggaaatacaa ttgcatcgtt acagttctaa ttttttcgac gcagcctgca ttgttccggt
     1441 gagagccaga ccagagctcc tggcgcaagc agtgatatct atcttgaaag gaagcactct
     1501 gccacgagaa atcatcctcg tgctcgatgg aactgcggaa gagagagctg cggatcaaaa
     1561 cggagtcaaa cagattacac atagcgcaga ggcagcctct gttcgtctgc gcgttttaga
     1621 aacgcccgca gttggcccgg ccgcggccag gaacaccgcg gtaagggcca cagaaatgcc
     1681 ctggcttacg tttcttgatt cagacgatct atgggagact cgcaagctcg agatgcaatc
     1741 tacatacatg cgaaagcgtc cgcatctact tgcctgccag acaggcgaaa gctggatgaa
     1801 aaacgacaag cctgtgcaca ggccgagaaa gctcactcct catactggac gtttcctgag
     1861 agatgctgtg cacagttgtc ttctttctct gtctgcgttc atgattcgcc gcgatgcgtt
     1921 tttggatgcg ggaatgtttg atcctgattt tgccctgtgc gaagattttg atttttccct
     1981 gcgatttctg cgttctcatg caattggata tctcgaagaa gatcttgtgg aaaaaaggag
     2041 cggctcctgg aaacaactca gtcagactcc tgcgatcgat gcatacagag tcagagcact
     2101 gtgcaaattg ttaggagatc acgggcgagc tggagaaggg ccgggttcaa ttgcagagcg
     2161 cgaaagatcg gaggccattc gatctctcac gcacaaagtg gccattcttg aaaagggcca
     2221 ggaaaaattt ggacagagcg cattcgttca gaatgcgatt cgagagggac gcgccgttct
     2281 cagtctttct tcagacgatc gatgacttcc ccgatatcta cgttgtattt cttccacact
     2341 gtgttgcgcg tcagttcgta tctcacaagt gagatgttga aattgatgcg t
//
LOCUS       DAORFF010000596         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_33251_length_2377_cov_3.347115, whole genome shotgun sequence.
ACCESSION   DAORFF010000596 DAORFF010000000
VERSION     DAORFF010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33251_length_2377_cov_3.347115"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PKK76_17925"
     CDS             <1..655
                     /locus_tag="PKK76_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70412.1"
                     /translation="IPELRHGQHRSGDYVYYRHKCRVGGWSDYVKESEFLEALELIIE
                     SIRFLDSLSDEVKELYREDLKNSDLVQARVRTGANRTISVANQRKAKLLDSYLDGVVS
                     QGDYKAKADELDSLIRGAQRELNRAILEAGDIFKNLESSIIALREFPQIFNAASKENR
                     LRTVRAFASAMVYNRKKEKFLLQWIEPFSFLLAPETLAVSASKDGLGFVLECAQERT"
     gene            complement(637..720)
                     /locus_tag="PKK76_17930"
     tRNA            complement(637..720)
                     /locus_tag="PKK76_17930"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(684..686),aa:Leu,seq:cag)
     gene            794..1393
                     /gene="hisB"
                     /locus_tag="PKK76_17935"
     CDS             794..1393
                     /gene="hisB"
                     /locus_tag="PKK76_17935"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNL70413.1"
                     /translation="MKARIAEAARKTSETEIQMKINLDGTGVQKFDMEIPFFEHMLTH
                     ISKHGCIDFDLKLRGDIGIDCHHSVEDTAIVLGGLIHQALGDKRGIKRYGHSIVTMDE
                     VLTTVAIDLGGRYHFQYTGPETIKEGKFGIYDAELSNEFLSKLAMNARMNLHVIVHYG
                     ENRHHIHESVFKALGRALRMAAELDDRATGIPSTKGMLE"
     gene            1483..2100
                     /gene="hisH"
                     /locus_tag="PKK76_17940"
     CDS             1483..2100
                     /gene="hisH"
                     /locus_tag="PKK76_17940"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNL70414.1"
                     /translation="MPGIAVLDYGMGNIHSILKALRLFHSDVTYTSDPARIRSSEALV
                     LPGDGAFEAAMEGLAPSRDLILQHVSEDKPLFGICIGFQVLFEDSSECESGIIPGLGL
                     LNGNIRRFQFQDPDIRVPHMGWNRLIPSSLADKEYYSDYMYFIHSYRAENTNPSEVVA
                     SCDYAGDTFPAVVRKDSILACQFHPEKSGDHGLFLIRDWVSSLSA"
     gene            2105..>2377
                     /locus_tag="PKK76_17945"
     CDS             2105..>2377
                     /locus_tag="PKK76_17945"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNL70415.1"
                     /translation="MFEIIPAIDLLDGQVVRLHKGDYDQKKVYSNDPPAFAREFVSAG
                     AGWIHVVDLNSARNGERSVNRSVLREIVQASGDARLQTGGGIRSSAD"
BASE COUNT          574 a          582 c          627 g          594 t
ORIGIN      
        1 gattcctgaa ctgcgccatg ggcagcacag gagtggagac tatgtctatt acaggcataa
       61 atgcagggtc ggggggtggt ctgactatgt gaaggaatcc gaattcttag aagcccttga
      121 attgattatt gaaagcatac ggtttctcga ctcactgtcg gatgaggtca aagaactcta
      181 tcgggaggat ttgaagaatt cggatctagt ccaagcaaga gtgcggaccg gagcaaatcg
      241 gacaatatcc gtggctaacc agcggaaggc gaaacttttg gacagctatc tggatggggt
      301 ggtttcacaa ggtgactaca aggccaaagc cgacgaactc gattcgctca tccgaggtgc
      361 ccagagggag cttaacaggg ccattcttga agcaggcgac attttcaaga acttggagtc
      421 gtcaattatc gcgctacgcg agtttccgca gatcttcaac gccgcgtcca aagaaaatcg
      481 tctccgaacc gtcagagcgt ttgcgagcgc tatggtctac aacaggaaga aggagaaatt
      541 cctccttcaa tggatcgagc ccttctcctt tctgctcgcc cctgaaacct tggcggtcag
      601 cgcctcaaaa gacgggttgg gtttcgtcct cgaatgtgcc caggagagga cttgaacctc
      661 cacaggattg ctcccaccag gacctgaacc tggcgtgtct accaattcca ccacctgggc
      721 gggcctgcca gttttttgaa actggcggct ggaatcaatc gattttgatc ctggccgttg
      781 cgatattata tagatgaaag cgagaatcgc agaggccgcc cgaaagacct cggaaacaga
      841 aatccagatg aagatcaacc tggacggaac gggcgttcaa aagtttgata tggagattcc
      901 gttcttcgag cacatgctca cgcacatttc caagcatggc tgcattgatt tcgatcttaa
      961 actgcgcggg gatatcggaa tcgattgtca tcattccgta gaagacactg ccatcgttct
     1021 gggtggattg attcaccagg ccctcgggga caaacgcgga atcaagcgct acggccactc
     1081 cattgttact atggacgaag tactcacgac tgtggcgatc gacctgggag gtcgttacca
     1141 ttttcagtac acgggccctg aaacaatcaa agaagggaag tttggcattt acgatgctga
     1201 actctccaat gagtttctat cgaagcttgc tatgaatgcg cgcatgaact tgcatgtgat
     1261 agttcactac ggcgaaaaca ggcatcacat tcatgaatct gtgttcaaag cgctcggccg
     1321 ggcacttcgc atggctgcgg aattagacga tcgggcgact ggaattcctt ctacaaaagg
     1381 catgctcgaa taacattttt gtgttttttt tgatccgcgc gcggtgtgca cgattttgaa
     1441 acgactttga cgcctggccc cctggcggga aattcggtat gtatgcccgg aatcgcagta
     1501 ctggattacg ggatgggaaa cattcactcc atcctgaagg ccctgcgtct ttttcactcc
     1561 gatgttacct acacttcgga tccggcccgc attcgttcca gcgaagcact cgttctgcct
     1621 ggcgatggtg cttttgaagc agcgatggaa ggacttgctc cttcgcgcga tctcattctg
     1681 cagcacgttt cggaagacaa accgttattc ggcatttgta taggttttca ggttctgttc
     1741 gaagattcaa gcgaatgcga atcggggatc attcccgggc ttggtctttt gaacggaaac
     1801 atccgccgct ttcagtttca agatccagac attcgcgttc cgcacatggg gtggaatcgt
     1861 ctcattcctt cttctcttgc ggacaaagag tactactcgg actatatgta cttcattcat
     1921 tcttatcgtg cggagaacac aaatccgtct gaagtcgtgg ccagctgtga ttatgcgggg
     1981 gatacgtttc ccgctgtagt gcgaaaagat tctatccttg cctgtcagtt tcatcctgag
     2041 aaatcaggcg atcacggact ttttctaatc cgggactggg tttcttctct gagcgcctaa
     2101 cgttatgttt gaaatcattc ctgccattga tctcctggac ggccaggtgg ttcgccttca
     2161 caagggcgac tacgatcaga aaaaagtcta tagcaatgat ccgcctgcat ttgcgaggga
     2221 atttgtttcc gcaggcgccg gatggattca cgtcgtggat ctcaattcgg cgcgcaacgg
     2281 cgagcgcagc gtgaatagaa gtgtactcag ggaaattgtg caagcttcag gagatgcacg
     2341 tcttcagaca ggcggcggca ttcgaagcag cgcagac
//
LOCUS       DAORFF010000597         2368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_33485_length_2368_cov_2.874189, whole genome shotgun sequence.
ACCESSION   DAORFF010000597 DAORFF010000000
VERSION     DAORFF010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33485_length_2368_cov_2.874189"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PKK76_17950"
     CDS             <1..443
                     /locus_tag="PKK76_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HNL70416.1"
                     /translation="RYPSMPYTKLKEDCDRYFYLPHRKEGRGIGGIFYDYVDTAPEVM
                     GQFTREAGSSFLDAYLPLLKKRSSQSYTEEDREFQMWRRGRYVEFNLVYDRGTQFGLK
                     TGGRIESILMSLPPIVRYKYDYRPAPGSRYEATMQALSNPRTWV"
     gene            434..1099
                     /locus_tag="PKK76_17955"
     CDS             434..1099
                     /locus_tag="PKK76_17955"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNL70417.1"
                     /translation="MGVRTIFIGDVHGCVDELKEMIGILGPTREDRIILLGDLINRGP
                     DSPGVVRFVFESGFESLMGNHEYEYLRHYTQIPVFKKIREGLGQELHSWLEGRPLFIE
                     TDAFLAVHAGLFPGKHPAETPEEVLLNIRTVRPEEGFDSDLNRNGDPAWHTYYKGTKP
                     VFYGHWARQGLTISHPTYGLDSGCVYGRALSAYVLETGELIQVPAKNVYYRKPYDSSP
                     ESG"
     gene            1099..1983
                     /locus_tag="PKK76_17960"
     CDS             1099..1983
                     /locus_tag="PKK76_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70418.1"
                     /translation="MMRLRKALHKIIRFSPAILLGLSWFCIPRPAKTDVRWKPVLSLP
                     RDAAIMQLRTEGAQDLLVYSGVQDGLSEIGGATDLLMSSGWNLLTLKTADRDPDISKA
                     FFEFAGNRPGFRRKILMVPGRDLCAASGSMEMSSLSALVVFRAEDSTFPDNECTTRLS
                     AIRPDLPVLFLVSPGLPGMSFVLYQAVPSSSKEYKTDSNMSPNVKTAFFQGRLLTFLH
                     LQRFPISFVESRTTFKKGCMMLPSGPWVIAFVSGQRAEGYCPLFLKSSGELYRAVDVR
                     PNAGCDYGVTSESAIRCQ"
     gene            complement(1986..>2368)
                     /locus_tag="PKK76_17965"
     CDS             complement(1986..>2368)
                     /locus_tag="PKK76_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNL70419.1"
                     /translation="LDEIGRGTSTYDGLSIAWAIVEALSDRRSLVLFATHYHELTALD
                     QKPGIFNLTMDVREIDGKIHFLRKVREGAADRSYGIHVAELAGIPESVLRRAKEKLFE
                     LESRPGKKAKSVRLKPAETEPGLF"
BASE COUNT          584 a          613 c          586 g          585 t
ORIGIN      
        1 atagataccc ctccatgcct tacacaaaac tgaaagaaga ttgcgatcgt tatttttatc
       61 tcccgcaccg caaggaaggt cgtgggattg gtggcatttt ctatgactat gtggatactg
      121 cgcccgaagt catgggccag ttcacacgcg aagcgggcag ctcgtttctg gatgcgtatc
      181 ttccactcct caagaaaaga tcctctcaaa gttacacaga agaagaccgc gagtttcaaa
      241 tgtggagacg cggacgttat gtggaattca acttagtata tgaccgggga acacagttcg
      301 gcctcaaaac gggcggaaga attgaatcca tcctcatgag tctgcctcca atcgtccgat
      361 acaagtacga ctacaggcct gcaccaggaa gcagatacga agctacgatg caggcactgt
      421 ccaatccaag aacatgggtg taagaacaat cttcatcgga gatgtccacg gctgcgtgga
      481 tgaactcaaa gaaatgatcg ggatactcgg tccgactcga gaggatcgta tcattctgct
      541 cggtgacttg atcaaccggg gtccagacag cccgggagtg gttcgcttcg tatttgagag
      601 cggttttgaa agcctcatgg gaaatcatga gtacgaatat ctcagacact acactcaaat
      661 tccagtgttc aaaaaaatac gcgaaggcct gggtcaggaa cttcactcct ggcttgaagg
      721 cagacctctg ttcatagaaa cagatgcatt cctcgccgtg catgctgggc tctttccagg
      781 aaaacacccg gctgaaacac cggaagaagt actgctcaac attcgaaccg tgcgcccgga
      841 agaaggattt gattctgatc ttaatagaaa cggcgaccct gcatggcata catactacaa
      901 aggaaccaaa ccggtattct acgggcactg ggccagacag ggtttgacta tttctcaccc
      961 cacctacggc ctcgattctg gatgcgtcta cggtagagca ctctctgcct acgttctgga
     1021 gacaggcgag ctgattcaag ttcccgccaa aaacgtatac tatagaaaac catacgattc
     1081 ttcaccggaa tcaggctgat gatgcgactc agaaaagcgc ttcacaaaat cattcggttc
     1141 tctcccgcca ttctgttggg cttatcctgg ttttgcattc ctcgcccggc taaaacagat
     1201 gtgcgatgga agccagtcct ttctctgccg cgcgatgcgg cgatcatgca actgcgcacc
     1261 gaaggcgcgc aggatctcct ggtctactcg ggagtccagg acggactttc tgaaattgga
     1321 ggcgccaccg acttactgat gagcagcggc tggaatctgc tgaccttgaa gactgccgac
     1381 cgcgatcctg atatttcaaa agcattcttt gagtttgcag gaaacagacc tggatttcgc
     1441 agaaagattc tcatggttcc aggccgagat ttatgcgctg cttccgggtc catggaaatg
     1501 tccagcctca gtgcgctcgt ggtctttcgc gcggaagatt cgacttttcc ggacaatgaa
     1561 tgcactactc gactttctgc cattcgcccg gatcttcctg ttttgtttct ggtttccccc
     1621 gggcttcccg gaatgtcttt tgtcttgtat caggcagttc caagctccag caaagaatac
     1681 aaaacagact ccaatatgtc gcctaacgta aagactgctt ttttccaggg acgactgctc
     1741 acttttcttc atcttcaaag gtttccaatt tctttcgtgg aatcgcgcac taccttcaag
     1801 aaaggttgta tgatgcttcc gtcgggccca tgggtgattg cttttgtcag tggacagagg
     1861 gcggaaggat actgtccctt gtttcttaaa tcaagtggcg agctctaccg cgcagtggac
     1921 gtgcgcccga atgcagggtg cgattacggc gtgacaagtg agtcggcgat tcgctgccag
     1981 tgaattcaga ataatcctgg ttccgtctcc gccggcttga ggcgaacgct ctttgctttt
     2041 tttcctggac gcgattcgag ttcaaatagc ttctctttgg cacgacgcaa cactgattct
     2101 ggaattccag caagctctgc cacgtgaatt ccataggatc gatccgccgc accttcgcgc
     2161 acttttctta agaaatgaat ttttccgtct atttctcgaa catccatggt gagattgaaa
     2221 atcccgggtt tctgatcgag agcagtcaac tcgtgatagt gagtggcaaa caggaccaga
     2281 gaacgacgat cggagagagc ctccacaatc gcccatgcaa tcgaaagccc atcataggtg
     2341 ctggtgccgc gcccgatctc gtcgagga
//
LOCUS       DAORFF010000598         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_33583_length_2364_cov_2.973582, whole genome shotgun sequence.
ACCESSION   DAORFF010000598 DAORFF010000000
VERSION     DAORFF010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33583_length_2364_cov_2.973582"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="PKK76_17970"
     CDS             <1..1158
                     /locus_tag="PKK76_17970"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNL70420.1"
                     /translation="HKDEDRERRQRNQLAAIALNNPALATTTTSSSLPPNLSTGRRLV
                     LHVPIFTGLKEYAGIEGSRALIDQRKYELLHDAGRLYLELAQDFYNVLVLERSLKTRT
                     ELLSLSRQNVDQLDRFVALGRVKRTEALQARSTVAKLEADVQAVQDNLTRSREALSLS
                     SGVRPDALFADAEPLAAPSLSRLQAEDIAESRADVMAARATLEVSKASLTQAQGEFAP
                     SFYVDGYYNLPRHNVPRNRDIIAQFTFEFPIFSGGSSIAGITKAESEKRTAQLTYDLL
                     KRQALLEIRQAFDAWESSRLQIAAYKKALDAAELNYGAQLDYYNRRLGTIVDLLAALT
                     TLASARDDYQKALLQERLNRVWVGVATGELPVRRKNQETVADKDHSKETAK"
     gene            1155..>2364
                     /locus_tag="PKK76_17975"
     CDS             1155..>2364
                     /locus_tag="PKK76_17975"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNL70421.1"
                     /translation="MNLVEVSIRRPVFAWILMLGLIFFGWIAFTKLGVSSMPDVDFPV
                     VNVQLALTGASPEVMETNVVDLVEDSLLSVEGIKQITSVSSYGQANITVELDLNRNVD
                     AALQEVQTRVAQAQKSLPTELEAPIITKFNPEDQPIMFLAVSSDKATTRDLMMYVRDH
                     LKTQFSVVPGVGQIVLGGFVDRNLRVWVDSRKLSARELTVEDVITAISREHKELPAGL
                     LETPMKQWNVRSMGEAPTADQFGNLPILYRGGAPMFDTLIRIRDVARVEDGLDEIRRI
                     SRFNGTSAVGIGIVKQRGTNAIDVAKAVKAKAAEIQKTLPPGYKIALAFDSTRFIEES
                     IGDLELTLFLSALLTSLVCFFFLGNWSSTINILLAIPTSIMGTFIVLYIMGFTLNTFT
                     LLALSLAIGIV"
BASE COUNT          604 a          578 c          598 g          584 t
ORIGIN      
        1 cacaaagacg aagatagaga aaggcgccag agaaatcaac tggctgcgat cgcactgaac
       61 aatcccgctc ttgcaaccac gaccacttct tcgagccttc cgccaaactt gagcactggc
      121 cgtcgtctgg ttttgcacgt gcccatcttt accggtctca aggaatacgc gggcatagaa
      181 ggatctcgcg ctctcattga tcaaagaaag tatgagctgc ttcatgatgc tggaagattg
      241 tatctggagc ttgctcagga tttctacaac gttctggttc tagaacgatc tttgaaaacg
      301 cgcacggaac ttctcagtct ttcgagacag aatgtggatc agctagatcg ttttgtggcc
      361 ctgggtagag taaaaagaac agaggcactg caggcgcggt ccactgtagc aaaactggaa
      421 gcagacgtgc aggcagtgca ggacaatctg acacgatcgc gcgaagctct atctctgtcg
      481 agcggtgttc gaccggacgc acttttcgca gacgcagaac cactggctgc tccttctctt
      541 tcgcggctgc aggcagaaga cattgcagaa tcccgtgcgg atgtgatggc agcgcgcgcc
      601 accctcgaag taagcaaggc cagtttgact caggcgcagg gagagtttgc tccttctttt
      661 tatgtggatg gatactacaa tcttcccagg cacaacgttc ctcgcaatcg agacatcatt
      721 gctcaattca ctttcgaatt tccgattttc tcaggcggat cctccattgc ggggatcaca
      781 aaagcggaat cagaaaaacg aactgcacag ttgacgtatg acttactcaa acgtcaggcg
      841 cttcttgaaa tcagacaggc atttgatgcg tgggaatcct ctcgtttgca aatagcagca
      901 tataagaaag ctctggatgc ggcggagttg aattacgggg cgcaactgga ctactacaat
      961 cgaaggctcg gcaccattgt agatctgctg gccgctctta ccacactcgc ttctgcgcga
     1021 gacgattatc agaaagctct tctacaagaa agattgaatc gagtctgggt gggtgtggcc
     1081 accggagaac tacctgttag gcgaaagaac caggagaccg tagctgataa agaccattcg
     1141 aaggagactg cgaaatgaat ctggttgaag tgtcgattcg aagacccgtg tttgcctgga
     1201 tccttatgct gggcttgatt ttttttgggt ggattgcgtt tacgaagctc ggtgtttcga
     1261 gcatgccgga cgttgatttt ccagtcgtca atgttcagct ggcactgacc ggtgcttccc
     1321 cggaagtgat ggagaccaat gtcgtagatc tcgtagaaga cagccttctt tccgttgagg
     1381 gaatcaaaca gatcacatct gtgtcgagtt atggtcaggc aaacatcaca gtcgaacttg
     1441 atctgaatcg taacgtggat gcagcccttc aggaagtaca gacaagagtc gcccaggcac
     1501 agaagtctct tcctaccgaa ctcgaagcac ccatcattac aaagttcaat ccggaagatc
     1561 aacccatcat gttcctcgcc gtttcaagcg acaaagcaac cacgcgagat ttgatgatgt
     1621 atgtgcgaga tcacttgaag acccagttca gcgtggttcc aggtgtgggt caaattgtac
     1681 tgggtggctt tgtggatcgc aaccttcgcg tatgggtgga ttcaagaaaa ctttccgcac
     1741 gcgaattgac cgtggaagat gtaatcactg cgatcagccg cgaacacaaa gaactacctg
     1801 cgggcttgct tgagactccc atgaagcaat ggaacgtgcg ttccatgggg gaagctccaa
     1861 cagcggatca gtttggaaat ctgccaattc tgtaccgcgg tggcgcgccc atgtttgata
     1921 cattgattcg cattcgcgac gtggcgcgcg tggaagatgg gctcgatgaa atcagacgta
     1981 tttccagatt caatggaaca tctgcggttg gaattggaat tgtaaagcag cgcggaacca
     2041 atgcaatcga tgttgcaaaa gcagtaaaag cgaaagccgc tgaaatacaa aagactctgc
     2101 ctcccggtta caagatcgca cttgcatttg attccactcg attcatagaa gaatcaattg
     2161 gagatctgga actcactctc tttctatccg ctctgctgac cagtctagtc tgttttttct
     2221 tcctgggcaa ctggagctct acgatcaaca ttcttctggc aattcccact tcgatcatgg
     2281 gaacattcat tgttctctac atcatggggt tcactctcaa tacgttcaca ctcctggctc
     2341 tttctcttgc aatcggtatt gtag
//
LOCUS       DAORFF010000599         2357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_33749_length_2357_cov_4.172024, whole genome shotgun sequence.
ACCESSION   DAORFF010000599 DAORFF010000000
VERSION     DAORFF010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33749_length_2357_cov_4.172024"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="PKK76_17980"
     CDS             complement(<1..708)
                     /locus_tag="PKK76_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNL70422.1"
                     /translation="MAEERRFRLVSEYKAAGDQPHAIAQLVDAYNQGKQKLCLVGVTG
                     SGKTYTMASFLEGIQKPALILTHNKTLAAQLYREFREFFPENAVEYFVSYYDYYQPEA
                     YVASSDTYIEKDASINEEIDRLRLRATASLLERRDVVIVSSVSCIYGLGSPEEYSHSL
                     VMIDQGLEIDRNDVIRQLLHIQYVRNDIAPARGTFRARGDNLEIFPAYRQEGIRVEWF
                     GDEIESIRLFDPISQKSV"
     gene            848..1306
                     /locus_tag="PKK76_17985"
     CDS             848..1306
                     /locus_tag="PKK76_17985"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNL70423.1"
                     /translation="MNNKYVFGAAGVVLGACLTVGAGAAMWRCYGPMGSPETRAAWVV
                     KKLSSDLDLDATQIQKLTAIKDEVLKHRAATASGREEMHKAVLAQVESANVDKAALNK
                     IFDSHMSDMKDSRVFLIDKFAEFHSILRDDQKKTLKKKMEHFLMSHDAPI"
     gene            1315..1824
                     /locus_tag="PKK76_17990"
     CDS             1315..1824
                     /locus_tag="PKK76_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004441932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNL70424.1"
                     /translation="MTEAEFTEIVESTKRVVLSAIEKNLYPRFLHAIDDVVQETYLRA
                     YKSLQKKQFEGRSALTTWLYTIARNESIRMNEKLGREDKKIAKLQAEPDREPAPQSDN
                     NSEDMMGWVKRLPARYMQVIKMYLGGSSEKEIADRLEISPGTVKSRAHRAREMMRKMM
                     EKEGQHAVK"
     gene            1811..2113
                     /locus_tag="PKK76_17995"
     CDS             1811..2113
                     /locus_tag="PKK76_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70425.1"
                     /translation="MQSSDQALKHEIESRCSSHEWTCNVARMVSGTVRRRIRIRNTVI
                     AVTFSVLLPAGLTASGHMERAAYALLDSMVGVRHQLVIQPVTTEEVDTLIEIALNN"
     gene            complement(2103..>2357)
                     /locus_tag="PKK76_18000"
     CDS             complement(2103..>2357)
                     /locus_tag="PKK76_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70426.1"
                     /translation="FGDVLEIAESETGLHVFAKWKKGIALFNRFEQSCLDHGIVPGSA
                     RRNYFTRYSPSLIFSVAHLSESQLTRALGQMAKILSAINY"
BASE COUNT          646 a          558 c          609 g          544 t
ORIGIN      
        1 gacggatttt tgggagatcg gatcaaagag ccggatcgat tcaatctcat ctccaaacca
       61 ttccactcga attccttcct gtctgtacgc tggaaagatt tcgagattgt cccccctggc
      121 gcgaaacgtc ccgcgcgccg gtgcaatgtc attgcgaacg tactgaatgt gaaggagttg
      181 tcgaatcacg tcattacgat ctatctccag accctgatca atcatcacaa ggctgtgact
      241 gtattcttcg ggagaaccca gaccataaat gcaagaaacg gaggacacaa tgacaacgtc
      301 gcgtctctca agcaagcttg ccgtcgcgcg aaggcgcaac cgatcgatct cctcgtttat
      361 ggacgcatct ttctcaatgt atgtatcgct cgatgctacg tacgcttctg gttgataata
      421 atcgtagtaa gaaacaaaat actccactgc gttttccgga aagaattccc ggaattctct
      481 atagagctgt gcagcgagag tcttgttgtg agtgagtatg agcgctggct tttgaattcc
      541 ctccagaaag gacgccatgg tatacgtttt acctgaaccg gtaacgccca ccaggcagag
      601 tttttgtttc ccttgattgt acgcgtccac cagctgtgct atggcgtgcg gttgatcccc
      661 cgctgctttg tattccgaaa ccagtctaaa tcttctttct tcagccacgg gagaaggaag
      721 ccaggaggca gacaacggtc aactgcaagg ggccattttt ttgctatctg gaggcgcggg
      781 actggttatt ttgagattcg cgggaacgaa tggtcctccc gccgggtctt acgattggga
      841 aacggcaatg aataacaagt atgtatttgg cgcagcgggc gtggttctgg gtgcctgcct
      901 gacggtgggt gccggcgcgg ctatgtggag atgctacggc cccatgggat cgcctgaaac
      961 tagagcggcc tgggttgtaa aaaaactttc atctgatctg gatctggacg ccactcagat
     1021 ccagaaactc acagccatca aggacgaagt tctcaaacac agagcagcca ccgccagtgg
     1081 tcgcgaagag atgcacaagg ctgtgctcgc ccaggtggag tcggcgaacg tggataaggc
     1141 agcactcaac aaaattttcg actctcacat gagcgacatg aaagacagca gagtgtttct
     1201 cattgataaa ttcgcagagt ttcattctat tttgcgagat gatcagaaga agacactcaa
     1261 aaagaagatg gaacacttcc tgatgtctca cgatgcgcca atctgatggg gacaatgacg
     1321 gaagcagaat ttactgaaat cgtcgaaagc acgaagagag tcgtactctc tgcgatcgag
     1381 aaaaatctgt acccgcggtt ccttcatgca atcgatgatg tagtgcagga gacgtatctt
     1441 cgtgcataca aaagcctgca gaagaaacaa ttcgaaggca gatctgcact gaccacctgg
     1501 ctttatacga tcgcacgaaa cgagtcgatt cgaatgaatg agaaacttgg tcgcgaagac
     1561 aagaaaatag ccaaattgca ggccgaacca gacagagagc ccgctcctca gtccgataac
     1621 aattcagaag acatgatggg atgggtgaag cggttgcctg cgcgatacat gcaagttatt
     1681 aaaatgtatt tgggcggatc cagtgaaaag gaaatcgcag atcgtcttga aatcagcccg
     1741 ggaaccgtga agtccagggc acaccgggca agagaaatga tgcgaaagat gatggagaag
     1801 gagggacaac atgcagtcaa gtgatcaagc attgaaacac gaaattgaga gcagatgctc
     1861 cagtcatgag tggacgtgca acgttgccag aatggtatca ggcacggtgc gaagaagaat
     1921 tagaattcgc aatacggtca ttgccgtgac gttctctgtt ctgcttcctg caggtttaac
     1981 cgcaagcggc catatggaaa gagcagcgta tgctcttctc gattctatgg ttggagttcg
     2041 tcaccagctg gtaattcaac cggtaaccac agaggaagta gataccctca tcgaaatcgc
     2101 tctcaataat tgatcgcgct cagaattttg gccatctggc ccagggcgcg agtcagttgc
     2161 gattctgaca aatgcgccac tgagaaaatg agagatggcg aataacgtgt aaaatagttt
     2221 cttcgcgcgg atccgggaac tatcccatga tccaggcagc tctgctcaaa ccgattgaat
     2281 agcgcgattc ctttcttcca ttttgcaaaa acgtgcaagc ctgtttccga ttcagcgatc
     2341 tcaagtacgt ccccaaa
//
LOCUS       DAORFF010000600         2352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_33876_length_2352_cov_3.358293, whole genome shotgun sequence.
ACCESSION   DAORFF010000600 DAORFF010000000
VERSION     DAORFF010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33876_length_2352_cov_3.358293"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PKK76_18005"
     CDS             complement(<1..622)
                     /locus_tag="PKK76_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70427.1"
                     /translation="MGRGLILSALTGSIALSYEILWARFYSFVSGAASNSFGILLASY
                     LFGLGLGALVAGIICRNVEGHSPELNRKLGSFVLFANLFGYIIAPAASWIVLFIPYLW
                     TLPLIVISAGLLGAAFPLISHITIQADARAGLRLSFVYLANIAGSSIGSLLTGFVLLD
                     YFSFSTISVILVIAGILLATLISKYRDPDNTEATVPARGSEFRRYGV"
     gene            complement(624..1247)
                     /locus_tag="PKK76_18010"
     CDS             complement(624..1247)
                     /locus_tag="PKK76_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70428.1"
                     /translation="MIRSLLIFVVFFVSGISALTYQLAWQRKLFSIFGTNIESITVVV
                     TAFMMGLGLGSLAGGILSQRKNFRVLPAFAIVELGIGVYGLLSMRLMESISNASSMSV
                     AMTGIVSFALVLVPTLLMGATLPLLVTHLVRGNQNVGQSVGLLYFANTIGAAFGALIG
                     ATIWFRFYGLSGSVHIAVILNFASAIVVGLLAITEKSISGKLEAEGK"
     gene            complement(1264..1587)
                     /locus_tag="PKK76_18015"
     CDS             complement(1264..1587)
                     /locus_tag="PKK76_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNL70429.1"
                     /translation="MLGTIPDKIEYDERELRILWKDGKECRFDLLSLRKNCPCANCRG
                     GHELTARRTTGHIQSIGLTSWKKVGRYALQLSWSDNHDLGIYTYDMLREACDKGTSYM
                     PPDER"
     gene            1938..>2352
                     /locus_tag="PKK76_18020"
     CDS             1938..>2352
                     /locus_tag="PKK76_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70430.1"
                     /translation="MLLGPAFLDFVFCFAGVRNRAFSMVAWITGLFWVSVFLFFPGLV
                     ISQTPTGFRMPLHFYAYTNYYPVFLANLFGIITYALFELLRNGLRMPVRASLMRFVLP
                     ACILWAACGFWDTTLSLLFRIPLPLSWAGGVVVNVA"
BASE COUNT          571 a          607 c          564 g          610 t
ORIGIN      
        1 agactccgta gcgtctgaac tcggatccgc gcgcagggac cgttgcttcc gtgttgtccg
       61 gatctctgta ttttgaaatc agcgtggcaa gcaagatacc cgcaatcacg aggatgactg
      121 aaatagttga gaaactaaag taatcgagca gcacaaatcc cgtcagcaag ctcccaatgg
      181 aggatcctgc gatgtttgcg agatagacaa agctcaagcg aagcccggct ctcgcgtctg
      241 cctgaatcgt aatgtgactg atcagcggaa atgcagcacc cagcaaccct gcgctgatga
      301 cgatcagagg cagtgtccac aagtagggaa tgaataaaac gatccagctt gctgccgggg
      361 cgatgatata tccaaacaga tttgcaaaca gaacaaacga tcctagcttt ctgtttagct
      421 cgggcgaatg tccttccaca tttctgcaaa tgattcccgc aaccagtgcg cccagtccca
      481 gaccaaaaag atagctggcc agaagaattc caaaactgtt ggatgcagca ccggaaacaa
      541 aagagtagaa tctggcccag agaatttcat aggatagtgc aatggaacct gtgagagcgg
      601 atagaatcaa tcctctgccc atattacttt ccttccgctt ccagttttcc ggagattgac
      661 ttttccgtta tggccaataa tcccacgact atggccgacg cgaaattcaa gatcactgct
      721 atgtgcacag atccactcaa gccataaaag cgaaaccaga tggtggcacc aatgagagct
      781 ccaaatgcag ccccgattgt atttgcgaag taaagtaacc ctacggactg acctacattt
      841 tgatttcctc gaactagatg agtcacgaga agtggaagtg tggcgcccat caagagtgtg
      901 ggaaccagca ccagtgcaaa tgatacgatg cccgtcatcg cgacgctcat gctagaggca
      961 ttgctgattg attccatcag tcgcatagag aggagcccgt atactccgat gccaagctca
     1021 acgattgcaa atgcaggcag tacgcgaaaa ttctttctct gcgaaagaat cccgccggcc
     1081 agactcccca gccccaatcc catcatgaat gcagtcacta caacggtaat ggattcgata
     1141 ttcgtaccaa agatggaaaa tagttttcgc tgccatgcaa gctggtaggt gagcgcggat
     1201 atcccggaga caaaaaagac cacaaaaatc aacaacgatc tgatcatgtt tcttctcttc
     1261 acatcatctc tcatccggag gcatgtaaga cgtacccttg tcgcaagcct ctcgcagcat
     1321 atcgtaggtg tagattccaa gatcgtggtt gtccgaccag gagagctgta aggcgtatct
     1381 acccactttc ttccacgaag tcaggccaat cgactggatg tgcccggttg tccttcgcgc
     1441 tgttagttca tgtccgcccc gacagttcgc gcacgggcag ttctttcgca ggctgagtag
     1501 atcaaatcgg cattcttttc catctttcca gaggatgcgc aattcgcgtt catcgtattc
     1561 aatcttgtct ggaattgttc caagcatgag ggccagttag cccctggtgc actctcagtc
     1621 aatgaatcac gcagaaatca ttttgtaaaa ctgcttaaac tgcttgaccg tcatcgtgaa
     1681 tgcgtcataa aaacggcgtc gatggagcta atcacactgg cgtctttcgg atttgcatgg
     1741 gcttccggag ccgcgtttct cattgcggtg tttgccgcat tcatgtccac gggtcggagc
     1801 gaaaatgagc tctggaggag attcgctttt ctttcggcag tcaataccgt tttctgcgcg
     1861 gtaagtgttg tccagaacca tccagagaat ttggaatcca tggcgttttt cacgcggctc
     1921 aatctggccg gtgcggaatg cttctggggc ctgcgtttct ggacttcgtg ttttgtttcg
     1981 cgggggttcg caatcgagct ttttccatgg ttgcgtggat caccggcctg ttctgggttt
     2041 ctgtgttttt gttttttccc gggcttgtca tttcacaaac tccgaccgga tttcgcatgc
     2101 cccttcattt ctatgcatat accaactatt atcccgtctt ccttgcaaac ctctttggaa
     2161 tcataacata cgctctcttt gaacttcttc gcaacggtct tcgtatgcca gtgcgcgcat
     2221 cgctcatgcg atttgttttg cctgcctgta ttctctgggc tgcatgcggt ttctgggata
     2281 caaccctgtc tctgcttttc agaattccct taccgctcag ctgggccggt ggagtagttg
     2341 tgaatgtggc gt
//
LOCUS       DAORFF010000601         2345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_34068_length_2345_cov_4.227948, whole genome shotgun sequence.
ACCESSION   DAORFF010000601 DAORFF010000000
VERSION     DAORFF010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34068_length_2345_cov_4.227948"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PKK76_18025"
     CDS             <1..571
                     /locus_tag="PKK76_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNL70431.1"
                     /translation="EEFRVATNKENAATGVKVTVLAFLIKASAMALKKHPEFNSSIDG
                     DNIVFKHYFHIGFAADTPAGLVVPVIRNVDQKSVTEIAAETSVLAKKAREGKLTPAEM
                     SGACFTISSLGGIGGTSFSPIINAPEVAILGVSKSAMKPVWNGKEFIPRLMLPLSLAA
                     DHRIIDGALATHFNVLIASLLSDVRRLVL"
     gene            588..>2345
                     /gene="lpdA"
                     /locus_tag="PKK76_18030"
     CDS             588..>2345
                     /gene="lpdA"
                     /locus_tag="PKK76_18030"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNL70432.1"
                     /translation="MSELITVKVPDIGNYKDIPVIDVCVKAGDTVKAEDALLTVESEK
                     ATMDIPSPVAGVVKEIKVKVGDRVSEGHAVAVIEASSTSSATSPAPSSSSANPTLASS
                     ASSSTAPAPTQGPAPSSAATASTYSGTVDMQCEMLVLGGGPGGYSAAFRAADLGLNTI
                     IVERYATLGGVCLNVGCIPSKALLHVAAVIEEASHIQVAGVQFAKPQVDLDKLKAHKN
                     GVVGKLTNGLSGMAKARKVEVVRGYGSFISANHLEVEITQGTAQDKTGTKKVIEFKQC
                     IIAAGSSAVHLPFIPIDPRVVDSTGALELRFVPGKMLVIGGGIIGLEMATVYSTLGSR
                     IDIVEMLGAIMAGPDRDLVKAWEKQNMHRFDQVMLNTKTVSVEAKPDGLWVKFEGENA
                     PKEPVRYDMILQSAGRSPNGKKIAAEKAGVQVNERGFIPVDLQMRTNVSHIFAIGDIV
                     GSPMLAHKAVHEGHVAAEVAAGQKSAFDAKVIPGVAYTHPEVAWVGVTEDQARAEGRA
                     VQTAKFPWAASGRAIANAAEYGFTKLIFDAETHRLIGGAIVGPSAGDMIGEVCLAIEM
                     GCDSVDIGKTIHPHPTLGET"
BASE COUNT          624 a          589 c          647 g          485 t
ORIGIN      
        1 ggaagaattt cgcgtcgcaa ccaataagga aaacgctgca acaggcgtga aggtcacggt
       61 acttgcattt ctaatcaaag caagtgcgat ggctctcaag aaacatcctg aattcaacag
      121 ctccatcgac ggagacaaca ttgtattcaa acactacttt cacataggat tcgccgcaga
      181 cacgcctgcg ggtctggtcg tgcctgtgat tcgcaacgta gatcaaaaat cggttacgga
      241 aatcgcggca gagacttcgg ttcttgcgaa gaaagctcga gagggaaaac tcactcctgc
      301 agaaatgtcg ggggcttgct tcaccatttc gagtctgggc ggaatcggtg gtacttcgtt
      361 ttcgcctatc atcaatgcgc cagaagtggc gatcctgggc gtaagcaaat ccgccatgaa
      421 gccggtgtgg aatggaaagg aattcattcc gcgcctcatg cttcctctct ctctggccgc
      481 agatcatcga atcatagatg gagcgcttgc cactcatttc aacgtgctca tcgcatcgct
      541 tctaagcgat gtgcgacgcc tggtactgta aggctacgag ggataccatg agcgaattaa
      601 ttacagtaaa agtcccggac ataggaaact acaaagatat ccccgtaata gatgtatgcg
      661 tgaaggcggg agatacggta aaagcggaag acgctctgct caccgtagaa tctgaaaaag
      721 caaccatgga catcccgagt cctgtggccg gagttgttaa agaaattaaa gtaaaagtgg
      781 gcgatcgggt ttcagaagga catgcggttg cggtcattga ggcatcgagc acctccagcg
      841 ccacatcgcc cgcgccctct tcctcatctg cgaatcccac tctggcctct tccgcctcct
      901 cttctacagc acctgcaccc acgcaaggcc cggcaccttc ttccgcagcc acagcttcca
      961 cctacagcgg cacggtggac atgcagtgcg aaatgctggt tcttgggggc ggaccgggtg
     1021 gctattcagc tgcctttcgc gcagcagatc tggggctgaa cacaatcata gtcgaacgtt
     1081 atgcgacact gggtggagta tgcttgaacg taggatgcat tccgtccaaa gcacttttgc
     1141 acgtcgcagc cgtcatagaa gaagcatctc atattcaggt cgccggagta caattcgcta
     1201 aacctcaggt ggatctggat aaactcaaag cacataagaa cggagtcgtg ggcaaactca
     1261 caaacgggct cagcggtatg gcaaaggctc gcaaagtgga agtagtgaga ggttacggaa
     1321 gctttataag tgccaaccat ctggaagtag aaatcacgca aggcacagcc caggacaaaa
     1381 cgggcaccaa gaaagtcatt gaattcaaac agtgcatcat agcagccggg agctctgcgg
     1441 ttcacctccc atttattccg atcgacccgc gcgtcgtgga ttcaacggga gcactcgagc
     1501 tcagatttgt tccaggcaag atgctggtca taggtggtgg aatcatcggc ctcgaaatgg
     1561 cgactgtgta ctctaccctg ggctctcgca ttgacattgt agaaatgctc ggagcgatca
     1621 tggcaggtcc ggaccgcgat ctggtcaagg cgtgggaaaa gcaaaacatg catcgctttg
     1681 atcaggtgat gttgaacacg aaaactgttt cagtagaagc aaagccagac ggactctggg
     1741 tgaaattcga aggagaaaat gccccgaagg agccggtgcg atacgacatg atcctccaga
     1801 gcgctggaag atcgcctaac ggtaagaaga ttgctgcaga gaaagcaggc gttcaggtaa
     1861 acgaaagagg attcattcca gtagatctac aaatgcggac aaatgtttcg cacatattcg
     1921 caatcggcga cattgttggc tcccccatgc tcgctcacaa agcagtgcac gaaggtcacg
     1981 tggcggcaga ggtggcggcc ggtcagaaga gcgcgtttga tgcgaaagtg attccaggcg
     2041 tggcatacac tcatcctgaa gtagcgtggg tgggtgtcac ggaagaccag gccagggcag
     2101 aaggacgtgc agtgcagact gcgaaattcc cgtgggctgc atccggacgc gcgattgcaa
     2161 acgcggcgga gtatggattt acaaagctca tctttgatgc agaaactcac cgcctgatcg
     2221 gcggagcgat cgtcgggcca tccgcaggcg acatgattgg agaagtgtgc ctcgccatcg
     2281 aaatgggatg cgattcagta gacattggaa aaaccattca tccgcatccg actctgggag
     2341 aaact
//
LOCUS       DAORFF010000602         2326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_34573_length_2326_cov_3.667988, whole genome shotgun sequence.
ACCESSION   DAORFF010000602 DAORFF010000000
VERSION     DAORFF010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34573_length_2326_cov_3.667988"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1171
                     /gene="pyrC"
                     /locus_tag="PKK76_18035"
     CDS             149..1171
                     /gene="pyrC"
                     /locus_tag="PKK76_18035"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNL70433.1"
                     /translation="MTIAMPDDFHVHLRDGVMLEGIVSHTAASFARAIVMPNLKPPIA
                     TTEEARLYRDRILAVAPGFTPLMTLYLTDRTLPDEVNRAKDSGFIHGIKLYPSGATTN
                     SESGVHDLEKLYPVLARMEQMDLPLLVHGEVTDTSVDVFDREKVFLDRHLKQLPRTFP
                     GLRIVFEHITTREAASFVMQSPKNVAATITPHHLLLNRNSMFRGGIRPHNYCLPVLKR
                     EEDRVALLDAATSGNQKFFAGTDSAPHERSTKENSCGCAGIYNAHAAVELYAEAFEEV
                     GKLDRLESFLSHHGADFYKLPRNKSTIRLAKKTQTVPDYYDFGGQKVVPMRAGETISW
                     SIERDS"
     gene            1161..1997
                     /locus_tag="PKK76_18040"
     CDS             1161..1997
                     /locus_tag="PKK76_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HNL70434.1"
                     /translation="MIRETIEMTTRVSDLDTQRHVTSRTYESFCLEGRHRLLEKQGYS
                     VGRLLDEGIHLVPTHSYVRFMYQQYAGAVLVIHTTAASDSKKGIIWDQKVEQLDGKPV
                     CHIQLRTEFFQGEDRLTLMDAGDAEFEILYEDLAEFKGGAQQIESSYTPLYCERDIFG
                     SYSPSLLWKVFEEGRWLFCARTGLTYEKFVEMDTTSFYMGGVFNFFGRMIAGKPLRIR
                     TWIDSLEKIRFYFRQDVFDGDRLLMSLRDEQLIVSLKNARPRKAPTEFISFIESYIEK
                     SK"
     gene            1994..>2326
                     /locus_tag="PKK76_18045"
     CDS             1994..>2326
                     /locus_tag="PKK76_18045"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNL70435.1"
                     /translation="MNSSNWLLYGATGFTGELIVEEAVRQGMKPVIAGRSEARIREMA
                     EKFGLEWKVFSLVDPQSIRAGIRGFACVLHAAGPFIYTAEPMMHACIDEGAHYLDITG
                     EIPVFQIAT"
BASE COUNT          587 a          559 c          600 g          580 t
ORIGIN      
        1 aattcaagtc ttccaccagg taaacaggcc cgcggatccg agtaattcgg atcggacgat
       61 cggacgcgtc gactggctcc ttgattgggc cgcatgacgc aattatcatt gcactcagcc
      121 agacgacgaa aagcctgagc atgtgcagat gaccattgca atgccagacg actttcatgt
      181 acaccttcgc gacggggtga tgcttgaggg aattgtttcc catactgcgg caagttttgc
      241 gcgggcgatt gtaatgccaa atctgaaacc acccattgcg acgaccgaag aggcgcgcct
      301 ctacagagat cgcatcctcg ctgtggcccc cgggttcaca cctctgatga ctctctatct
      361 gacggaccgt actctgccgg atgaagtcaa tcgcgcgaaa gattcgggtt ttatccacgg
      421 aatcaaactc tacccgtccg gagccaccac caattcagaa tccggagtcc atgatctaga
      481 gaaactctat ccagtgcttg cccggatgga acagatggat ctgcctttgc tggttcacgg
      541 cgaagtgaca gatacatccg tggatgtgtt tgatcgagag aaagtctttc tggatcggca
      601 cttgaagcag cttccacgaa catttccagg ccttcgcatt gtattcgaac acatcacgac
      661 tcgtgaagcc gcatcttttg tgatgcagtc tccgaaaaat gtggccgcta ccattacgcc
      721 acaccatctg ttattgaatc gcaattctat gtttcgcgga ggaattcgtc cgcataacta
      781 ctgtttgccg gtactgaaaa gggaagagga tcgagttgcg cttctggatg cggccacgtc
      841 gggcaatcag aaattcttcg ccggaacaga cagtgctccc cacgagcgat ccacaaaaga
      901 aaattcgtgt gggtgtgccg gaatctacaa cgctcacgcg gcagttgaac tttacgcaga
      961 ggcatttgag gaagtcggga agctggatcg tctggaatcg tttctgtccc atcatggtgc
     1021 tgatttctat aaactgcccc gcaacaaaag caccatccga ctggcgaaaa aaacgcagac
     1081 ggtgccggat tactatgact ttggcggcca gaaggtagtg cccatgcgcg ccggagagac
     1141 tatttcttgg agcatagagc gtgattcgtg aaaccataga aatgacaacg cgtgtttcag
     1201 acctggacac gcagcgtcac gtgaccagtc gcacgtatga aagtttttgc ctggaaggaa
     1261 ggcatagact gctcgaaaag cagggctatt cagtcggacg acttctggac gaaggaattc
     1321 atcttgtccc cactcattca tatgttcgat tcatgtacca gcagtatgct ggcgctgttc
     1381 ttgtgattca taccactgca gcctccgata gcaagaaagg aattatctgg gatcagaagg
     1441 tggagcagtt agacggcaaa cctgtctgtc atattcaact gcgtacggaa tttttccagg
     1501 gagaagatcg tctaacgttg atggatgcag gggatgcaga atttgaaatt ctgtatgagg
     1561 atcttgctga attcaaggga ggagcgcagc agattgaaag ttcgtacact cctctgtatt
     1621 gtgaaagaga tatttttggt tcttacagtc cctcgcttct ctggaaagtc tttgaagaag
     1681 gtcgatggtt attttgcgca cgcactggct tgacctatga gaaatttgta gaaatggaca
     1741 ccacatcctt ctacatgggg ggagtgttta acttcttcgg aagaatgatc gctggcaaac
     1801 ctcttcgcat tcgtacctgg attgattccc tggagaaaat ccgattctat ttcaggcagg
     1861 acgtatttga tggagacagg cttctcatgt ctttacgcga cgaacagttg atagtttctc
     1921 tcaagaatgc caggcctcgc aaagctccca cagaatttat atccttcatt gaatcataca
     1981 ttgagaaaag taagtgaatt cgagtaactg gttgttatac ggagctaccg gttttaccgg
     2041 tgagctcatc gtggaagaag cggtccggca aggaatgaag cctgtgatcg ccggccgatc
     2101 ggaagccaga atccgcgaaa tggcggaaaa attcggactg gaatggaagg tcttttccct
     2161 ggttgatccg cagtccattc gcgccggtat tcgtggattt gcatgcgtac ttcatgcggc
     2221 cggtcctttt atttataccg cggagcccat gatgcatgcc tgcatcgacg agggcgctca
     2281 ttacctggac attacaggag aaattcctgt gtttcaaatc gccacg
//
LOCUS       DAORFF010000603         2324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_34626_length_2324_cov_3.577788, whole genome shotgun sequence.
ACCESSION   DAORFF010000603 DAORFF010000000
VERSION     DAORFF010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34626_length_2324_cov_3.577788"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1675
                     /locus_tag="PKK76_18050"
     CDS             <1..1675
                     /locus_tag="PKK76_18050"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70436.1"
                     /translation="LGDSYCLIGPYNPETAPFEFEESAGDNVFAKACQKLKLSGLDAR
                     TGRWLIPGRPRVVLLNLASIAPQLSEIKYFLWQNHQIQAGKDDLVDQVLLFGAITRKF
                     LDALIEEAGSTRIVAHFHEWMAGTALPGIRRDSVPLATTFTTHATLLGRYLAQADHRY
                     FERLPFVDVDSEIRRFNIEVRSRLEMACAHGAHVFTTLSDITKEECTYLLKRKPDILT
                     PNGIHVERPVALHEFQNLHVAFKERIDRFVIGHFFPTYTFDLDKTLYFFSAGRYEYRN
                     KGFDLLLDALARLNRRLRESGSEKTVVAFIITKRPYHTINPAVFANFAMTDELRRVCE
                     EIQQRIGDRLFHETALGNRPALESLVEEYWWIRLRRTTHAWKKNTLPPVITHNLVDDA
                     HDDVLNQIRYLSMFNAQSDRVKVIYHPDFLSETNPLFGMDYEQFSRGCHLGIFPGSYE
                     PWGYTPLECLVRGVPAVTSDLAGFGSYVKQNIPSHQNSGLYVLARRGVEFQNASTELT
                     AILENYCRLERRDRIVLRNRCEALSDRFDWMDLASAYFEAHGMALSRTK"
     gene            complement(1678..>2324)
                     /locus_tag="PKK76_18055"
     CDS             complement(1678..>2324)
                     /locus_tag="PKK76_18055"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL70437.1"
                     /translation="PYYFSEAVQRMFRGSAPAFFVASFFFYLCYQLVDYYIGRFLEMR
                     HGISAVYLGWGLSVFLEILLMPFTALLFEKFHTRSLFVLAIAAGALRFLWLGVAATSE
                     IPAVFAQLLHGLHFAGYYAGVIFLLRRYFPAHLYGTGHALFSGFAMSCGGIAGNMLTA
                     YMLGKSPGVSSFAIVFLGAAGIHILLIGAFYFMRLPGGAVSHSARTEAENPPAV"
BASE COUNT          628 a          634 c          573 g          489 t
ORIGIN      
        1 actgggggac tcctactgcc tcatcggccc ctacaacccg gagaccgctc cctttgaatt
       61 cgaagagtcc gccggcgaca acgtatttgc aaaggcatgc cagaaactca aactgtccgg
      121 cctcgacgcc agaaccggtc gatggctcat cccgggccgc ccgcgagtcg tgctactgaa
      181 cctggcgtcc atcgctccgc aactctccga gatcaaatac ttcctctggc agaatcatca
      241 gatccaggcg ggcaaagacg atctggtcga tcaggtgctg ctgttcggag ccatcacccg
      301 aaaattcctg gatgcgctca tcgaagaagc aggcagcacc cgcatcgtcg cgcacttcca
      361 cgagtggatg gccggtaccg ctcttccggg gatccgtcgt gattccgtgc ctctggccac
      421 cacatttacg actcacgcaa cacttctcgg aaggtacctg gcgcaggcag atcacaggta
      481 tttcgaaaga ttgcctttcg tggacgtgga tagcgaaatt cgtagattca acattgaggt
      541 tcgcagtcgc ctcgaaatgg cctgtgctca cggcgcgcac gtatttacaa cgttaagcga
      601 cattacgaaa gaagaatgca catacttgct gaaaaggaaa ccagacattc tgactccaaa
      661 tggaatccac gtcgaaagac cggtggcgct ccacgaattt caaaatttgc atgtcgcgtt
      721 caaagaacgc atcgatcgtt ttgtaatcgg acatttcttt ccaacataca ccttcgatct
      781 cgacaaaacc ctgtacttct tcagcgcagg aagatacgaa taccgtaaca aaggattcga
      841 tctgctcctg gatgcgcttg ccagattgaa ccgcagattg cgcgagtctg gctcagaaaa
      901 aacagttgtg gcgttcatca tcaccaaacg tccgtatcat accatcaacc ccgcagtgtt
      961 tgcgaacttc gcgatgacgg atgaactcag acgagtctgt gaggaaatcc agcaaaggat
     1021 cggagatcgt ctcttccatg aaacggcgct cggcaacagg ccagcattgg aatcgctcgt
     1081 ggaggagtac tggtggattc gcttgcgtag aacaacgcat gcctggaaga agaacacact
     1141 ccctcctgtt ataacacaca atctcgtaga cgatgctcat gacgacgttc tcaaccagat
     1201 acgatatctg agtatgttta acgctcagtc ggatcgcgta aaggtcatct accatccaga
     1261 ttttctcagc gagactaacc ctttgtttgg aatggactac gaacagtttt cccgcggctg
     1321 ccacctggga atatttccag gatcctatga gccgtggggc tacactcccc tggagtgtct
     1381 cgtgcgcggt gttcccgctg taacaagtga cctcgccggg tttggatcct atgtaaaaca
     1441 gaatatccca tcgcaccaga atagcggcct ttacgttctc gcaagacgcg gcgtcgaatt
     1501 ccaaaatgct tccacagaat tgacagctat tcttgagaac tattgcagat tggaacgtcg
     1561 tgatcgcatc gtcctgcgaa atcgatgcga agccttatcg gatcgattcg actggatgga
     1621 tctggcttcc gcttattttg aagctcacgg gatggctctt tcgcgcacga aataagacta
     1681 aacggccggt gggttttctg cttctgttcg cgcgctgtgc gatacagcgc cgcccggcag
     1741 cctcatgaaa tagaaagcac caatcaagag aatgtgaatt cccgccgcgc caagaaaaac
     1801 aatcgcaaac gaggacacac ctggcgactt tccaagcata tatgcagtga gcatgttgcc
     1861 cgcgattcct ccacagctca tggcaaaccc ggaaaagaga gcgtggcctg ttccgtacag
     1921 atgagcaggg aagtatctgc gcagcaggaa aatcactccg gcatagtagc ccgcaaaatg
     1981 taagccgtga aggagttgtg cgaagaccgc aggaatttcg gaagtagcgg ccacaccgag
     2041 ccacagaaaa cgaagcgcac ccgccgcaat ggcgagtacg aacaatgacc gggtatgaaa
     2101 cttctcaaat agcagggccg tgaacggcat cagtaatatt tcaaggaaaa cagaaaggcc
     2161 ccagcccaga tacaccgcag aaatcccatg acgcatctca aggaatcgtc caatgtagta
     2221 gtcaacaagc tggtaacaca gatagaagaa aaaggatgca acaaaaaaag cgggagcgct
     2281 tccgcgaaac atcctttgca ccgcttctga aaaataatac ggct
//
LOCUS       DAORFF010000604         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_35517_length_2293_cov_3.728776, whole genome shotgun sequence.
ACCESSION   DAORFF010000604 DAORFF010000000
VERSION     DAORFF010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35517_length_2293_cov_3.728776"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKK76_18060"
     CDS             complement(<1..282)
                     /locus_tag="PKK76_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70438.1"
                     /translation="MDRLKQALMESDSQELNLVKDRVEVIGASIAGCLARAASHLGQE
                     VSRLDYEILERGKSTLLTKIPYRLLVSVLPPEERFADLEEFSIKLGVGDR"
     gene            complement(282..1037)
                     /locus_tag="PKK76_18065"
     CDS             complement(282..1037)
                     /locus_tag="PKK76_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70439.1"
                     /translation="MDFERRIQESVARKKILIKSYLLREEGEEKLERIIHAVLRSCGR
                     EDLMGPIYACMRELVQNAAKANLKRVVFEEQGLNPFDEDQYRFGLEAFRKSLASKRIQ
                     HYRPILHARDVPFYVKIKWDGRVLLLNVENYFAMLTVEEKRIREKFIHSHNMDNLYEF
                     FMTYGDSTEGAGMGIAMVQILMKQAGFDAHNFTIFSDPIRNRTSARLVVPLVKDYSEP
                     RVRFAEEMKRRGVEASVLRKEIQEGKLEFPVFY"
     gene            complement(1037..>2293)
                     /gene="thrC"
                     /locus_tag="PKK76_18070"
     CDS             complement(1037..>2293)
                     /gene="thrC"
                     /locus_tag="PKK76_18070"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNL70440.1"
                     /translation="YLCDCGGLLEVRHDMAAIRSRSASEWKTLFTERYRSSEYPFQSG
                     VWGKKEWVLPHISNAEIVSLGEGATPMVGVPRFARELGLGSLSVKQCGVSHTGSFKDL
                     GMTVLVSHVVSLRAKGIPIHAVACASTGDTSAALAAYAASAGIPTVVFLPAGKVSNAQ
                     LVQPVSSGSLVLSLETDFDGCMQIVKEVTANKTLYLANSMNPLRIEGQKTISFEITQQ
                     MGWEVPDWVVIPGGNLGNVSALAAGFEMLLETGIISKRPRILLAQSARANPMYLSYKN
                     NYRYSPVQASQTLASAIQIGNPVSYPRARRALEKYNGIVEQATEQELADAAARADLHG
                     LFNDPHTGVALAGLIKCRERGDIEADARVVVISTAHGLKFQEFKAGYHEGTLADVSGK
                     YRNSVKKLRANVSDVQSALEEFLRVN"
BASE COUNT          557 a          645 c          531 g          560 t
ORIGIN      
        1 tctatctccc acacccagct tgatcgagaa ctcttctaga tctgcgaagc gctcctctgg
       61 aggcaggaca gaaaccagaa ggcgataggg aatctttgtc agtagagtgg acttacctct
      121 ttccagaatc tcatagtcga ggcgtgatac ttcctgacca aggtgagatg cagcgcgagc
      181 caggcaacca gcgatgctcg caccgatcac ttccacccgg tcctttacca ggttcagttc
      241 ctggctgtcg gactccatca atgcctgttt cagccgatcc atcagtaaaa cacgggaaac
      301 tccagtttcc cttcctgtat ttcttttcgg aggactgagg cctccactcc tctccttttc
      361 atctcttccg cgaagcgcac ccttggctca gaataatcct tcaccagcgg aaccacgagc
      421 ctggcagagg ttcggttgcg aatcgggtcc gaaaatatgg taaaattatg agcgtcaaag
      481 cccgcctgtt tcatgaggat ctgcaccatt gcgattccca tcccggcgcc ttccgttgaa
      541 tctccatacg tcataaagaa ttcgtacaga ttgtccatgt tgtgagaatg aatgaatttc
      601 tctctgattc ttttctcttc cacggtaagc atggcgaagt agttctcaac gttgagaaga
      661 agcacacgtc catcccattt gatcttcacg tagaaaggca catcgcgtgc gtgtagaatc
      721 gggcggtaat gctgaattct cttggaggca aggcttttgc ggaacgcctc gagaccaaag
      781 cgatactggt cttcgtcgaa aggattgagg ccctgctctt caaaaacaac gcgcttcagg
      841 tttgccttgg ccgcgttttg aaccagctcg cgcatgcagg catagattgg acccatgagg
      901 tcttcgcgtc cgcaagaacg caacacagcg tggatgatcc tctcaagctt ctcctctccc
      961 tcctctcgaa gtagataaga tttgatcaga atcttctttc gcgcgacaga ctcttgaatt
     1021 ctacgttcaa agtccatcag ttcaccctca agaactcctc gagcgctgac tggacatccg
     1081 atacgtttgc acggagcttc ttcacagaat tgcgatactt cccggacaca tcggccagtg
     1141 taccttcgtg gtaccctgcc ttgaattcct gaaacttcaa gccatgtgca gtgctgatta
     1201 caaccacgcg agcatccgcc tctatgtctc cacgctctct acatttgatg agccctgcaa
     1261 gcgccacacc tgtgtgagga tcattgaaca atccatgcaa gtctgcgcga gccgccgcat
     1321 ccgccagctc ctgctccgtt gcctgctcaa cgattccatt gtatttttca agtgcgcgac
     1381 gcgcacgcgg gtagctcacg ggatttccga tctgaatggc agaggccaat gtctgggatg
     1441 cttgcacggg cgaataacgg tagttgttct tgtaactcag gtacattgga tttgccctgg
     1501 cactctgtgc gagaaggatg cgcggtctct tcgaaatgat tccagtttcc aggagcattt
     1561 caaaaccggc ggcaagcgcg ctgacatttc ccaggtttcc gcccggaatg acaacccagt
     1621 ctggcacttc ccatcccatt tgctgcgtaa tttcaaaact gattgtcttt tgtccttcga
     1681 tgcgaagagg attcatcgag ttggccagat aaagcgtctt gtttgcagtg acttctttta
     1741 caatctgcat gcatccgtca aaatcagttt ccagactcag aaccagactt ccagacgaca
     1801 ctggctgcac aagttgcgca ttggatactt tacctgctgg aagaaagaca accgttggaa
     1861 ttccggcgga agcagcatac gccgcgagag cagcactcgt gtctcctgta gaagcacagg
     1921 caactgcatg aattggaatt ccctttgcac gaagagaaac aacatgactt acaagcacag
     1981 tcatacccag atccttgaat gatcctgtat gactcacccc acattgcttt acggaaagcg
     2041 aaccaagccc cagctcacgc gcaaagcgag gaacacccac catcggagta gcgccttctc
     2101 ctagagaaac gatctccgca tttgaaatat gaggaaggac ccattccttc tttccccaga
     2161 ccccggactg aaaaggatac tcagaagagc gataacgttc cgtaaacagg gtcttccatt
     2221 cggaagccga tctggaacgg atggcggcca tgtcgtgacg aacttcgagc aatcctccgc
     2281 aatcacagag gta
//
LOCUS       DAORFF010000605         2290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_35613_length_2290_cov_3.648770, whole genome shotgun sequence.
ACCESSION   DAORFF010000605 DAORFF010000000
VERSION     DAORFF010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35613_length_2290_cov_3.648770"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PKK76_18075"
     CDS             <1..571
                     /locus_tag="PKK76_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNL70441.1"
                     /translation="PTIGYRGMHSFEVNFDNYFVPAENLIGGEAGRGKGFYLQMEGFA
                     GGRVQTAARANGVMQASLEAAVRYANDRKVFSKPIADYQLTKWKIARMAMIVQASRQY
                     SYEVARLMDNHQGLMEASLVKFYASRIAEWVAREAMQIHGGMGYAEEYAVSRYFVDAR
                     VFSIFEGAEEVMALRVCARDLLAQALGQS"
     gene            641..856
                     /locus_tag="PKK76_18080"
     CDS             641..856
                     /locus_tag="PKK76_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70442.1"
                     /translation="MDVRSKPEIGMKNPEMFRDLKARKLENPAALPTTPDSERQMVTD
                     EKIAKSVLVSQSKDVFKVTGTQIDKIA"
     gene            complement(933..1298)
                     /locus_tag="PKK76_18085"
     CDS             complement(933..1298)
                     /locus_tag="PKK76_18085"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNL70443.1"
                     /translation="MILLEGLHHISIGSSDLKKSLDFYRDLLDFELLEESSRYILIRL
                     DPITVRLNHIEGYKSPVQNPGEASLSFILDVDDFTDALKELEVKKVEILKGPVMIEGG
                     EAVLIADPDGNLIELFYNE"
     gene            1400..>2290
                     /locus_tag="PKK76_18090"
     CDS             1400..>2290
                     /locus_tag="PKK76_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70444.1"
                     /translation="MMLRLLLALALFPGLAAAGPVRFEFKLEKDDVLAVDKYQDIRIQ
                     KNGEQKSREEKNRIVLKVSDSTPSGAVMEGLFHTYTRSPRLVGEYRRDRDFNSRFIFQ
                     KNGENVVSDDYVMPNLRGLPTFPDKELSAGDKWQAPGLETMDFGSAKIKIPLNVAYQF
                     TGVTHLPEDSKLSQDAPEIQYVYAFRKAISDPRVPFKMIVGHSADRLWFDTNAGVPIF
                     DTNRLTYTFYMRDGQILRMAYRIDSWWKKTRRARPEDRQEIVTDVNRQIAENPNVTVH
                     QTDEGVVLNLNAILFDVDSAN"
BASE COUNT          637 a          558 c          575 g          520 t
ORIGIN      
        1 cccaacgatt ggttatcgcg ggatgcacag cttcgaagtg aactttgaca attacttcgt
       61 tcctgcagaa aacctgatcg gtggagaagc aggacgaggc aaaggattct atcttcaaat
      121 ggaaggtttc gcaggtggcc gcgtgcaaac tgcagcgcgc gcgaacggag tcatgcaggc
      181 atccctggaa gcagctgttc gctacgcgaa tgatcgcaaa gtattttcaa agccaatcgc
      241 agactaccag ctcactaagt ggaagatcgc gcgcatggcc atgatcgttc aggcttctcg
      301 ccagtattcc tacgaagtgg ctcgcctcat ggacaaccac cagggactca tggaagcatc
      361 tctcgtgaag ttctatgcat ctcgcatcgc ggaatgggtg gcacgcgaag ccatgcaaat
      421 ccatggcggg atgggctacg cggaagaata cgcagtatcg cgctacttcg tagacgctcg
      481 cgtgttctcc atctttgaag gcgcagagga agtgatggca ctgcgagtct gcgcgagaga
      541 tctactggcc caggctctgg ggcagagctg aggtaaggaa ttggcaaaaa aggctggatc
      601 gatttgaacc agccttctat ttttgcgaaa ggaggaaccc atggacgttc gatctaaacc
      661 agaaatcggc atgaaaaacc cggaaatgtt tcgggacctc aaggcacgta agctggagaa
      721 cccggcggca ctgcctacca cacctgactc agaaagacag atggtgacgg atgaaaagat
      781 cgcaaaatcc gtgctcgtaa gccagtccaa agacgtattc aaagtgaccg gcactcaaat
      841 agacaagatc gcctaactct tctttcaaaa tcatggcaca aaaaaaccgg ccctgaaagc
      901 cggtttcatt tcatcatccg tagatgatct catcattcgt tgtagaaaag ttcgattaga
      961 tttccgtctg gatctgcgat cagaaccgct tccccgccct caatcatcac aggacccttg
     1021 agaatctcga ccttttttac ttcgagttct ttgagcgcat ctgtaaagtc gtctacgtcc
     1081 aggatgaaag aaagggatgc ctcaccagga ttctgaaccg gggatttata tccctcaatg
     1141 tggttgaggc gcacagtgat cgggtcaagc cggatcaaga tataacggct ggattcttcg
     1201 aggagctcga aatcaagcaa gtcccggtaa aaatcgaggg atttctttag atccgaagat
     1261 ccaatagaaa tatggtgaag tccttcgagg agaatcatgc tataccccgc atccagtcag
     1321 aattgaacca ggctcagggc gatcaacgat tttccggaaa gttgctatca agattttcaa
     1381 ccgaccgtcg aatgattcaa tgatgcttcg actgctgctt gcgcttgctc tttttccggg
     1441 tcttgcggct gccggcccgg tccgtttcga attcaaatta gaaaaagacg acgttctggc
     1501 tgtggacaaa taccaggaca ttcgcattca aaagaatggc gaacagaaaa gcagagaaga
     1561 aaagaatcga atcgtgctga aagtctctga tagcactcct tcgggcgctg tgatggaggg
     1621 cctctttcac acctatacca ggtcgcctcg ccttgtgggc gaatacagaa gggaccggga
     1681 tttcaattcc agattcattt ttcaaaagaa tggcgaaaac gttgtcagcg acgattacgt
     1741 aatgcccaat cttcgcgggc tgcccacgtt tcctgacaaa gaactctccg ccggtgacaa
     1801 atggcaagca cctggactcg agacgatgga cttcggtagc gcgaaaatca agattccatt
     1861 gaacgtagcc taccaattca caggggtgac tcacttgcca gaggattcaa aactctcgca
     1921 ggatgcgcct gaaatccagt atgtctatgc gtttcgcaaa gccatcagcg atccgcgcgt
     1981 tccttttaag atgattgttg gtcattctgc ggacagactg tggtttgata ctaacgcagg
     2041 cgtacccata tttgacacaa acagactcac gtatacgttt tacatgcgcg acggccagat
     2101 ccttcgcatg gcttacagaa ttgattcatg gtggaagaaa accagaagag caagaccaga
     2161 agatagacag gagattgtaa cagacgtcaa caggcagatc gcggaaaacc caaacgttac
     2221 ggttcatcaa accgatgaag gcgtggttct aaacctcaat gcaattctat ttgatgtaga
     2281 ctctgcgaat
//
LOCUS       DAORFF010000606         2286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_35724_length_2286_cov_3.909458, whole genome shotgun sequence.
ACCESSION   DAORFF010000606 DAORFF010000000
VERSION     DAORFF010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35724_length_2286_cov_3.909458"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..408
                     /locus_tag="PKK76_18095"
     CDS             127..408
                     /locus_tag="PKK76_18095"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNL70445.1"
                     /translation="MRSVSDAAKWEAIASMLTRRATLPACYFTFPLLTTTPESYNGRY
                     RVAEAERLMKAHPEKTLIEIAFESGFNSKTSFHVGFKKLRGVAPSKFGR"
     gene            412..1194
                     /locus_tag="PKK76_18100"
     CDS             412..1194
                     /locus_tag="PKK76_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70446.1"
                     /translation="MSRYTEELKKKAARLQESDAKRRAFWLSVNQWVERLFQKLANII
                     ERQNSKKNDRFLVWWRIRTIDPVVGMFVSSALITLLILTFTIPISEYQTMLLAGSTGD
                     RIALAAGVAGYIVWFWGIYDAVRFKYWRRRCPFEIRGWENLVDDPELEHERWRPCEIH
                     ITLAIDSPDIRAGLTALYDVFAIEANRLYYNADSEDDGKKRIPWMSSGTDATGSANSR
                     VLWKIYKLLSEHLGKFHRKTKAIASVSITAGASFNVSHPSSD"
     gene            complement(1312..1776)
                     /locus_tag="PKK76_18105"
     CDS             complement(1312..1776)
                     /locus_tag="PKK76_18105"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNL70447.1"
                     /translation="MEPKGTRPGLTAMKLKNKIFLGLLALGSVAFFANCRHHSIEDRS
                     KWVTGKISDKLDLDKSQEAQLDKIRDEVVAKVKAQKPQRDQLAQEFQTLVKADKLDKT
                     KLKDLQTKHDALHAEIENLVIDRMAELHASLKPEQREKAANAIQKLRDHMAR"
     gene            1835..>2286
                     /locus_tag="PKK76_18110"
     CDS             1835..>2286
                     /locus_tag="PKK76_18110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70448.1"
                     /translation="MIYFALFFVYFLGNGAYFSGFSPYVLGNYPAESAAIFLSSQIAS
                     PAGTLLAGVTTDKTRLLRWPLFVCVSSLVVFQFLFFSVLSPLWTIVSSICLRFFLSAA
                     GQILVIATLESGGARFARARTAGTVGFCVIQIVLYLLAETLVAHSSAE"
BASE COUNT          495 a          553 c          572 g          666 t
ORIGIN      
        1 cttcgttgaa tgagtaaaga tgaatatgaa gacctttgcg atcagttgaa gagcaaatct
       61 cgaatccatc ccgggttgaa acacgcccgg gctttcccag aaaatacgcg atgctctgct
      121 gcatcgttgc gctctgtctc ggatgctgca aaatgggagg cgattgcatc gatgctgacc
      181 cgtcgtgcga cgctgccagc ttgctatttt accttcccct tgctcacaac aacgccggaa
      241 tcttacaatg gacgctaccg ggtagccgag gctgaacgac tgatgaaagc acatccggaa
      301 aagactctga tcgaaattgc gtttgagtct ggtttcaatt caaagacatc tttccatgtt
      361 ggttttaaga aattgcgcgg agtagctcct tctaaatttg gtagatgatc tatgagccgc
      421 tatacggaag agctaaaaaa gaaagccgcg cgcctacagg agtcggacgc gaagcgcagg
      481 gcattctggc tctcagtgaa ccagtgggtg gaacgtctat tccagaaact cgcgaatatc
      541 atcgaacggc aaaactcaaa gaagaatgat cggttccttg tctggtggag gattcgcacg
      601 attgatcccg tcgttggaat gttcgtatcg tcagctctca tcactctttt gattcttacc
      661 ttcacgatcc ccatatccga ataccaaaca atgttgctcg cgggaagtac gggggatcgc
      721 atcgctcttg ctgcaggtgt ggcaggatat attgtgtggt tctggggcat ctacgatgcg
      781 gttcgcttta agtactggcg cagacgttgt ccattcgaga ttcgcgggtg ggaaaacctg
      841 gtagatgatc cggagcttga gcatgaaaga tggagaccgt gcgagattca tatcactctg
      901 gcaattgatt cgccggacat acgtgccggc cttaccgccc tttacgatgt tttcgcaatc
      961 gaagccaatc gcctttacta caatgcggat tctgaggacg atggaaaaaa gcgaattcca
     1021 tggatgagtt caggtaccga tgcaacgggc tctgctaatt cgcgcgtctt gtggaagatc
     1081 tacaagctgt taagtgaaca ccttgggaaa tttcaccgca aaacaaaagc aatcgcttcc
     1141 gtgtcgatca cggctggagc atccttcaat gtatcgcatc ccagttcgga ttgagatcct
     1201 actttctttt tagattcgca ctcattttgg ccggcgtggc gaaaaaaatc ccggcagtgt
     1261 tactgccggg tgctttgatg gtcttacgcg tttgcaagcc tcacattgca ctcatcgtgc
     1321 catgtgatcg cggagctttt gaatcgcatt tgctgctttc tcgcgctgct ctggcttcag
     1381 agaagcgtga agttcagcca tacgatcaat cacgaggttt tcaatttcgg catgaagtgc
     1441 atcgtgtttt gtctgaagat ccttcaactt tgtcttgtct agtttgtcag ctttcaccag
     1501 agtctgaaat tcttgcgcaa gctggtcgcg ctgaggtttt tgagctttca cctttgctac
     1561 aacttcgtcg cggattttgt ccagctgagc ttcctggctt ttgtccagat ccagtttgtc
     1621 tgaaatcttt ccagtgaccc atttgcttct gtcttcaatg ctatggtgtc tgcaatttgc
     1681 gaagaacgcc acagagccca gggcaagcag gccgaggaat atcttattct ttagcttcat
     1741 ggccgtaaga cctggcctgg ttcctttcgg ttccatttgt gtctctgtga acatacgatt
     1801 gcatgctctg cttgccaaat ttctctgaca tgcattgatc tacttcgcgc ttttttttgt
     1861 ttactttctg ggtaacggag cttatttctc tggcttcagc ccgtacgtgc tgggcaacta
     1921 tccggcagaa agcgccgcca tttttctttc gtcacagatt gcatcgccgg cgggaacttt
     1981 gcttgcgggc gtgacaacag acaagacgcg tttgctccgg tggccgctat ttgtttgcgt
     2041 ttcttccctg gtggtttttc aattcctgtt tttttctgta ctatctcccc tgtggacaat
     2101 cgtgagttcc atatgcctgc gtttcttttt gtccgctgca ggacagatcc tcgtgattgc
     2161 gacactggag agcggcggag ctcgatttgc acgcgcgaga actgcaggaa cggttggttt
     2221 ttgtgtgatc cagatcgttc tctatcttct tgcagaaacg ttagtcgctc attcgagtgc
     2281 ggaatt
//
LOCUS       DAORFF010000607         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_35788_length_2284_cov_3.256617, whole genome shotgun sequence.
ACCESSION   DAORFF010000607 DAORFF010000000
VERSION     DAORFF010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35788_length_2284_cov_3.256617"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1891)
                     /locus_tag="PKK76_18115"
     CDS             complement(<1..1891)
                     /locus_tag="PKK76_18115"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNL70449.1"
                     /translation="MGVFLRLVKYSFRYRGRFLAGLGVAFLVAVLSAVSLNSFLPFFE
                     ALGDKSAEFALPSSRAERNLMKKAILASLKDKPLHVLILIEALKYPSEDKAWTDFLGL
                     TQPRGKEGKLDKVKSRILLALLPDKPTGVTDIESFQLRQIIYWKLRVNAYGFRPLEVI
                     YGVLMILVPLIALRLVLQIVTVRLIAKAGYRAVRDLRSDLYRKVQELPLTFFYSQKSG
                     VILSRMINDAEVVAAVISSNLRDAITNVFILVTHFAVLAYLNLQLLLVSLIAVPLMLS
                     PVTLFARKVRKSTGKSQEYLADLNALLKETISGVKVIRSFAMEGYEAEQFRRANQKLY
                     WRTFKQQFYLRASPNLVELTSAVVALGMLGLGAFFLDPTDFTGGQFMAFLVILLSLIR
                     PVIQLSGMMAKVGQGSEAGRRIFEILDRPRDIEEARHPAVLRPLRKHLEFKNVSFQYP
                     ETDKLVLENINLHVEAGQTIALVGESGSGKSTLMDLLARFFDPTSGSIRIDGTDIRDF
                     RIADHRSRIGIVQQENFLFHGPVRENIAYGRPEISIREIMKAARLAHAHDFIKELPGK
                     YESMIGERGLNLSGGQRQRIAIARALLRDPEILILDEATSALDTQSERLVQHALDRLF
                     RNRTVF"
     gene            2008..>2284
                     /locus_tag="PKK76_18120"
     CDS             2008..>2284
                     /locus_tag="PKK76_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70450.1"
                     /translation="MPRFEPFPFTADIADNFRAKKQIPAHLYSKEGQILIYRKDGATD
                     GEIDKILKFAERGIYYNVDDAESLGLKGDRRDVPAGLSDTKLFASEKA"
BASE COUNT          588 a          586 c          580 g          530 t
ORIGIN      
        1 caaaaactgt gcgattgcgg aaaagccggt cgagagcgtg ctggaccagg cgctcagact
       61 gggtgtccag agcggacgtg gcctcgtcca gaatgagaat ttccggatcg cgtagaagag
      121 cccgcgcaat ggcgatcctc tgtctctgac cgcctgaaag gttcagccct cgttctccaa
      181 tcatggattc gtattttccg ggcaattctt tgatgaagtc gtgagcgtga gcaagccggg
      241 cggccttcat gatttcgcga atcgaaatct cgggccgtcc atacgctatg ttttcacgta
      301 cgggaccatg aaacagaaaa ttttcctgct gcacgattcc gattctgctt ctgtgatctg
      361 cgatgcgaaa gtcgcgaatg tctgttccat cgattcgaat gcttccgctg gttggatcaa
      421 aaaagcgcgc gagcaaatcc atcagagtgg acttgccact tccgctttct cccaccagtg
      481 cgatggtctg gcccgcctct acatggagat tgatattttc gagcacgagt ttgtccgttt
      541 caggatactg aaaggaaaca tttttgaact caagatgctt tcgcaacggg cgaagcacag
      601 caggatgtcg cgcctcttct atgtctctgg gtctgtccag aatttcaaaa atgcgtctac
      661 ctgcttccga tccctgcccc actttcgcca tcataccgga gagctggatc actggccgga
      721 tcagagaaag aagaatcacc aggaatgcca tgaactggcc gccggtaaaa tcagttggat
      781 ctagaaagaa cgctcccagt ccgagcatcc cgagtgcaac gactgcactg gtcagttcta
      841 caagattcgg tgatgcgcga agatagaatt gttgcttgaa agttcgccag taaagtttct
      901 gattcgcgcg acggaactgt tctgcttcgt aaccttccat ggcgaaagaa cgaatcactt
      961 tcacgcctga gatcgtttct tttagaagag cgttgagatc ggcgagatat tcctggcttt
     1021 tccctgtaga ctttcttacc tttcgcgcaa atagagtcac aggcgaaagc atcaaaggta
     1081 ctgcaatcag agatacaagc aagagctgca gattgagata ggcaagtacg gcaaagtgag
     1141 tcaccagaat aaaaacgttc gtgatcgcat ctctcagatt gctcgaaatc acagcggcca
     1201 ctacttcggc atcattgatc attctactga ggatcacacc tgacttctga ctgtagaaga
     1261 atgtaagagg aagctcctgc accttgcgat atagatccga tcttaaatca cgaacggctc
     1321 tgtagcctgc cttcgcaatc aatcgcaccg ttacaatctg aagcacgaga cgaagagcaa
     1381 tgagcggtac cagaatcatc agtacgccgt agatcacttc gagcgggcga aatccgtacg
     1441 cattgaccct gagtttccag tagatgatct gtctgagctg aaacgattca atgtccgtaa
     1501 cgccagtggg cttgtctgga agcagagcga gcaatatgcg cgatttgact ttgtctagct
     1561 tcccttcctt gccgcgaggc tgggttaacc ccaggaagtc tgtccatgcc ttgtcctcgg
     1621 agggatattt caatgcttct atgaggatca agacgtgcaa cggtttgtct ttgagagagg
     1681 caaggatcgc cttcttcatc agattgcgtt ctgcgcgaga cgaaggcagc gcgaactcgg
     1741 cggatttatc cccgagcgcc tcgaaaaagg gcagaaagga attgaggctg accgcactga
     1801 ggactgcgac caggaatgcg acgccaaggc cagcaaggaa gcggccccgg taacgaaacg
     1861 aatatttcac caggcggaga aagacgccca cagtctatta tcccgcccgc ggctttcccg
     1921 gcaatccaaa caagcctgta caaatccaga ctggcagagc gcattttttt taagtgaagg
     1981 cctgcccacg tttgtttttt ttgacttgtg ccacgatttg aaccgtttcc gtttacggca
     2041 gacatcgcgg acaattttcg cgcgaaaaaa caaatcccgg cccaccttta cagtaaggaa
     2101 gggcagatcc ttatttacag aaaggatggg gccactgacg gcgaaataga caaaattctc
     2161 aaattcgcag agcgcggcat ctactacaac gtggacgacg cagagtccct cggtctcaaa
     2221 ggagacaggc gcgatgttcc cgccgggctc agcgatacaa aactatttgc atccgaaaag
     2281 gcac
//
LOCUS       DAORFF010000608         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_36115_length_2272_cov_2.976545, whole genome shotgun sequence.
ACCESSION   DAORFF010000608 DAORFF010000000
VERSION     DAORFF010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36115_length_2272_cov_2.976545"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2272
                     /locus_tag="PKK76_18125"
     CDS             <1..>2272
                     /locus_tag="PKK76_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNL70451.1"
                     /translation="IGKIARLTQEIKKEESPLQKQLKGTVYAIALLAFTLGAAFLALG
                     WVFGGLSFLQAFVFFIGLFVANVPEGLLPTVTLSLAMGVSRMAKRNALVKNLSSVETL
                     GCTTVICCDKTGTLTQNLMMVTQIYADGKTISVDGIGYEPMGSFHSESGPISSDELSR
                     KPVLTGLFSCAYNCNNARIEKHANAYRVLGDPTEGALVALANRAGVRGTHSRLHLNPF
                     ESVRKRMSVVVRRDGDIRKTVYAKGAPMEMLERCKLIQEGNNTRLLTEGDRAAIKEKN
                     DEFASSGLRVLALAFRNDDGIQDISEYNAENCETDLVFLGLAAMSDPIRPQVPGAIEI
                     CHAAGIRVIMITGDYALTAQSIGKRIGLGSGAAGGNLPVFAGVDIAQMTDESLAEILK
                     AGETVFARVSPDQKLRIVTLLRNLGEIVAVTGDGVNDGPALKRADIGIAMGLRGTDVA
                     KEAAQIILTDDNFASIAAAIEEGRGIFENIKRFAAYVLSSNPQEMYPYILWMLFPGTP
                     LAMTVMGVLAVDVGTDLIPAMGLGIEPPEKGIMEKPPRKKGEKLLSVGFILQNYFVQG
                     SILAFACFATYFYFGWALGVFSGGVSLSHLPSSPAGLDMKLASFVYLQSLTAYFFPTV
                     ATQIGNVMCRRSSRASLFSKDFLSPYYREEILNSLESWKPSRYTASLQIDAKMSRITD
                     RAAWSSMIQALAFSALLPLRLARVALQDIVVGLYRPLLRPLQKLAHAILKKQHVILNL
                     VSNPLINLGIAFELFLC"
BASE COUNT          527 a          600 c          585 g          560 t
ORIGIN      
        1 atcggcaaga ttgcccgcct gacccaggaa atcaaaaaag aggaaagccc tctccagaaa
       61 cagctcaaag gaacagtgta tgcaattgca cttctggctt ttactctggg tgctgccttt
      121 ctcgcactcg gctgggtatt cggaggcctc tcttttctcc aggctttcgt ctttttcatt
      181 ggcctgttcg ttgccaacgt gcccgagggg ctactcccca ccgtaactct atctctggcc
      241 atgggtgttt cgcgcatggc aaagcgaaac gctctggtga aaaatctgtc ttctgtggaa
      301 acacttggat gcaccactgt gatttgctgc gataaaacgg gcactctgac acaaaacctg
      361 atgatggtca cgcagatcta cgctgacgga aagaccatct cagtagacgg aattggctac
      421 gaacccatgg gaagctttca ctctgaatca gggcccatat cctccgacga actctctcgc
      481 aagcctgtgc ttaccgggct cttttcctgt gcctacaatt gcaacaatgc gcgcattgaa
      541 aagcatgcaa acgcctatcg agtgttaggc gatccaaccg aaggagccct ggtcgccctg
      601 gccaatcgcg ctggtgttcg aggaacccac agcagattgc atctgaatcc ttttgaatct
      661 gtgcgaaagc ggatgagcgt ggttgtgcgg cgagacggag atatcagaaa aacagtctat
      721 gccaaaggcg cacccatgga aatgctcgag cgctgcaaac tgatccagga aggaaacaac
      781 acacgtttgc ttacggaagg ggaccgcgcc gccatcaaag agaagaatga cgaatttgca
      841 tccagtggcc ttcgcgtcct ggcacttgca tttcgcaatg acgatggtat ccaggacata
      901 tcagaataca acgcagagaa ttgtgagacc gatcttgttt ttcttggtct ggcagccatg
      961 tcagatccaa tacgcccaca ggttccaggc gccattgaaa tctgtcacgc agctggcatc
     1021 cgtgtcatta tgatcacagg ggactacgct ctgaccgcac agagcattgg aaaacgcatt
     1081 ggcctgggtt caggagccgc cggggggaac ctgcccgtgt ttgccggtgt ggacattgcc
     1141 cagatgacag acgaaagcct cgcggaaatc ttgaaggccg gcgaaactgt gttcgcacgt
     1201 gtttctccgg atcaaaaact tcgcatcgtc actcttcttc gcaatctggg ggaaattgta
     1261 gcagtcactg gagatggagt caacgacgga ccggcactga aacgggcgga cattggaatt
     1321 gcgatgggcc ttcgcggcac cgacgtggcc aaagaagcgg cacagatcat tctcacagac
     1381 gacaactttg cctcgatcgc agccgccatt gaagaaggcc gtgggatttt cgagaacatc
     1441 aaacgatttg ccgcctatgt tctttcgagc aatccacagg agatgtatcc ttacatcctg
     1501 tggatgcttt ttcctggcac gcctctggcc atgactgtca tgggagtgct cgctgtggat
     1561 gtgggcacgg atctgattcc tgccatgggg cttggaattg agccgccaga gaaaggaatc
     1621 atggagaagc ctcccagaaa aaaaggggag aagcttttgt ctgtgggctt tattctgcag
     1681 aactatttcg tccagggaag catccttgcc tttgcctgct ttgctaccta cttctatttt
     1741 ggatgggctc ttggtgtttt ctcaggtgga gtctcactga gtcaccttcc ctcttcgcct
     1801 gcgggcctgg acatgaagct cgcatcgttt gtgtatcttc agtctctaac agcatatttc
     1861 tttcccactg tagccacaca gattggaaac gtgatgtgca ggcggtcttc acgcgcctcc
     1921 ttgtttagca aagatttcct gagcccctac tatagagagg aaattctcaa ctcccttgaa
     1981 tcatggaagc cgtcccgtta tacggcatct ctccagatag acgcgaagat gtcgcgcatc
     2041 acagacaggg ctgcctggtc gtccatgatc caggcgcttg cgttttctgc tttgcttcca
     2101 ttgcgtcttg caagagtcgc actgcaggac attgtggtgg ggctatacag gcctctactg
     2161 cggcctctgc aaaagctcgc tcacgcaatt cttaaaaagc agcatgtaat cctgaatctg
     2221 gtctcgaatc ccttgatcaa tctgggaatc gcctttgagt tattcctttg ct
//
LOCUS       DAORFF010000609         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_36146_length_2271_cov_3.007220, whole genome shotgun sequence.
ACCESSION   DAORFF010000609 DAORFF010000000
VERSION     DAORFF010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36146_length_2271_cov_3.007220"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /gene="flgB"
                     /locus_tag="PKK76_18130"
     CDS             complement(<1..335)
                     /gene="flgB"
                     /locus_tag="PKK76_18130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNL70452.1"
                     /translation="MRSMQMLEMGLSATSARRDVIANNIANVDVPNFKRSEISFEADL
                     KRAVDSERSFAEQPLLKTLHPGHIDGRRPIDYRSVQPTSHTDYLSTMRADGNNVDMED
                     EMSKMVRNQ"
     gene            428..811
                     /locus_tag="PKK76_18135"
     CDS             428..811
                     /locus_tag="PKK76_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70453.1"
                     /translation="MKSLSRILPTPSARSLALILLAALAAGSCRSVYRNHDPAVPSTQ
                     TATLSQSGFVFRLTALKNKVSMSDTLSLKPGDYMIDFQLPSTGDGGVARCVLDAGKVY
                     KFKIVERRYLPEIGMHALVGECAIK"
     gene            798..1790
                     /locus_tag="PKK76_18140"
     CDS             798..1790
                     /locus_tag="PKK76_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70454.1"
                     /translation="MRSSNRSLRKVTRGLAFVLIACGIAEATLRFIPGVDFYLKENSL
                     HCAASFYLRLCPSRSIEFKNSAGETYTVTTNEAGERITSALRDTSRQDSRTTQLELWL
                     IGDSIAMGHGLTDSDTAAWIAQSRLKSWKVRNLATDSVGPLAMTDILKQTPGKPAQII
                     WIFNPSDFIDDPLETARRESFPRRAIFILKYYVRSSAIASAARIIAGSSDASYVQGGS
                     SPLPDAADPIFAHILELSKEAGNHGARFCILLYSDVEAGANKPEKENRLRSMIADLAL
                     AHHICTIDVRGQFQSSKENLYLQNGHPGPAASRIFADAIVNSVSGQEPRAKSEQ"
     gene            complement(1738..>2271)
                     /locus_tag="PKK76_18145"
     CDS             complement(1738..>2271)
                     /locus_tag="PKK76_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNL70455.1"
                     /translation="LTAFGIAPAVVMYSFALRGINPVVEGITLPIGMALSAVYPACAA
                     YRLARFNVAHDPTSFQGLPSPVAGVIVALMPLGLQGSFQIPVALMVVIFVGVAFLMVS
                     TIKYSKPQATVFKRFNPVRLGILIAFIVIALIAVGILRSFAISAALLFMIVLVYGISG
                     VIALILHAVLGQRQNSQ"
BASE COUNT          575 a          607 c          560 g          529 t
ORIGIN      
        1 atttgattgc gaaccatctt ggacatttcg tcttccatat ccacgttgtt gccgtctgct
       61 cgcatcgtgg aaagatagtc ggtgtgcgat gtgggttgca cgcttctata gtcgatcggg
      121 cgccttccgt caatgtggcc cgggtgcaaa gtcttgagca aaggctgctc tgcgaaggat
      181 ctctctgaat ccacggctct tttcaggtct gcctcgaagg aaatctcgct tcttttgaag
      241 ttcggaacgt ccacattggc gatgttgttt gcgatcacat ctcgcctcgc ggaggttgca
      301 gaaaggccca tttcgagcat ctgcatactt ctcatgcctg gtccgtcaaa catcgacata
      361 tttagacttt cggccggtat ataaaaagcc ttgatgaatt atgtcacccg cccgacattt
      421 ttcatagatg aaatcccttt ccagaatact tcccacccct tctgcacgct ccctcgctct
      481 gatccttctg gcagccctgg ccgcaggaag ctgcagaagc gtctacagga accacgatcc
      541 tgccgttccc agcactcaaa ccgcgacgct gtctcagtcc ggcttcgtgt tcagactgac
      601 tgcgctcaag aacaaggttt ctatgtcaga caccctcagt ctgaagcccg gggattacat
      661 gatcgatttc cagcttcctt ccacgggaga cggtggagtg gccagatgcg ttctggacgc
      721 gggcaaagtg tacaagttca agattgtaga aaggagatac ctgcccgaaa tcggaatgca
      781 tgctctcgtc ggcgaatgtg cgatcaagta accggtcttt gcgaaaagtc acgcgcggcc
      841 tggcattcgt tctgattgca tgcgggatcg cggaagcaac ccttcgattc attccgggcg
      901 tggatttcta tctgaaagaa aattcgctgc actgcgccgc ctctttttat ctcagactct
      961 gcccttccag gtcgattgaa tttaagaact ccgccgggga aacatacact gtaactacaa
     1021 acgaagccgg agagagaatc acttccgcat tgcgcgacac atcgcgccag gacagccgaa
     1081 cgacccagct tgaactatgg ttgatcggag attccattgc aatgggacac ggcctcactg
     1141 attcagatac ggccgcctgg atcgctcagt ccagattgaa gtcgtggaag gtcagaaatc
     1201 tggccacgga ttctgttgga ccactggcta tgacagacat tctaaaacaa actccaggga
     1261 agcccgctca aataatatgg atctttaacc cgagcgactt catagacgat cctctcgaaa
     1321 cggcacgacg cgaatctttc cctcggaggg ctatctttat tcttaagtac tacgtaagat
     1381 caagcgcaat tgcatccgcc gcgagaatca tcgctggaag ttcagatgct tcgtacgttc
     1441 agggtggatc ctctcctctc ccggacgcgg cagatccgat cttcgcacac attctggaac
     1501 tctcgaaaga agctggcaat cacggggctc gtttttgcat tctgctttat tcagacgttg
     1561 aggcaggcgc aaataaaccg gaaaaagaaa atcgtttacg atctatgatc gcggatctgg
     1621 ctctggcaca ccatatctgc acgatcgacg tgaggggtca gtttcaaagc tcaaaagaga
     1681 atctgtattt acagaacgga caccccggcc cggctgcttc tcgcattttc gcggatgcta
     1741 ttgtgaattc tgtctctggc caagaaccgc gtgcaaaatc agagcaatga ctccgctgat
     1801 tccataaacc agaacaatca taaacaacaa cgccgcagat atggcaaagc ttctcagtat
     1861 ccccactgca atcagtgcaa tgacaatgaa ggcgatgagg attccaagac gcactggatt
     1921 gaatcttttg aataccgttg cctgcggctt agaatacttg atggtgctta ccattaagaa
     1981 ggcaactccc acaaaaatca caaccatcag agccactgga atttgaaagg agccctgcag
     2041 accaagaggc atgagtgcca caatgacgcc ggcgacggga gatggaagtc cctggaaaga
     2101 agttggatcg tgtgcgacgt tgaaccgagc aagcctgtag gcggcacagg caggatacac
     2161 cgcagacaga gccatgccga tgggtagagt gattccctcc acgaccggat tgattccgcg
     2221 cagtgcgaaa gaatacatca caacggccgg ggcaattcca aatgcagtca a
//
LOCUS       DAORFF010000610         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_36297_length_2266_cov_3.316147, whole genome shotgun sequence.
ACCESSION   DAORFF010000610 DAORFF010000000
VERSION     DAORFF010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36297_length_2266_cov_3.316147"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PKK76_18150"
     CDS             <1..883
                     /locus_tag="PKK76_18150"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNL70456.1"
                     /translation="SVTIMRGCDNFCSFCVVPYTRGRERSRSIDSIVEEVSRLIEEEG
                     VREVMLLGQNVNSYKYEGHDFAALVERLLSSTSIARIRFTSPHPHDFPDHLLSLMASE
                     ERFCSQIHLPVQSGSTSVLSRMKRDYSREEYLALVAKMKDRIPEVGLSTDIIVGFCGE
                     TESEFEDTLSLVKECRFDVAYMFRYSEREGTFAKKKMPDDIPEEVKLERLARLISVQS
                     ESSLAKNQEEIGKVHRVLVEGRSKRSAADNSGRSDSGKKIIFPAAGTKPGEIVPVRIE
                     SVTSATMRGTVVAPVFS"
     gene            918..1904
                     /locus_tag="PKK76_18155"
     CDS             918..1904
                     /locus_tag="PKK76_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70457.1"
                     /translation="MNRLISILLFLWLAPLLADRDVHGDISSAEVSRYLDVRLDLDRL
                     AVQPGQKVMVRYRVTNQGYRIFRIFPHEDARRTFQFLVEDKAGKEVAPRERPEDASEQ
                     PVRDLVGNRVKEILLHPGETFEKTMDLNAMYDLSPGQEYRVCAYFVPDQESDLAIRSK
                     NWARVRVSKMERAPAAREQDAAVSSDSGLSPEETVYLFLSAEIKHNWPAYLKHVDLPR
                     FVGSYDRFALKYAQASDLERPGVLREFSTYLTRRDGDDLKRFRILRTDLDRDKSGEIL
                     PTGNARVTVQADRDTEGFRVRYEYVYTLKRAEQEGFWKIIHVSARVVRQGQS"
     gene            1957..>2266
                     /locus_tag="PKK76_18160"
     CDS             1957..>2266
                     /locus_tag="PKK76_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNL70458.1"
                     /translation="MTEILSQDEIDALLSAISTGEVEMEDYSNVGEQKKVKIYDFRRP
                     DKFSKDQIRTLQMMHETFARLTTTALSAQLRALVGVHVGSVDQLTYEEFIRSIPNPTT
                     L"
BASE COUNT          561 a          548 c          608 g          549 t
ORIGIN      
        1 atccgtaacc atcatgcgcg gttgcgataa cttctgttcc ttttgcgttg ttccttacac
       61 gagagggaga gagagaagcc ggtcgataga ttccattgtg gaagaagtca gcaggctgat
      121 cgaagaagag ggcgtgcgcg aagtcatgct tcttggccag aatgtgaact cctataaata
      181 cgaaggccat gattttgcgg ctcttgtgga aaggctcctt tcttctactt ccatcgcgcg
      241 tatccgcttt acttctcctc atccgcatga ctttccggat catcttctgt ccttgatggc
      301 atcggaagag cgcttctgct ctcagattca tctaccggtt caatccggat ccacctctgt
      361 tctttctcgc atgaaacgag attattcgcg cgaagagtat ctggctctgg ttgcgaaaat
      421 gaaagacaga atcccggagg tcggactgag cacggacatc attgttggct tctgcgggga
      481 aacggaatct gagttcgagg ataccctcag tcttgtgaaa gaatgcagat tcgacgtcgc
      541 ttacatgttc cggtattcgg agcgcgaagg tacgtttgcc aaaaagaaaa tgccggacga
      601 tattccggaa gaggtcaagc tggaacgcct ggcccggctc atttccgttc aatccgaaag
      661 ctcccttgca aaaaaccagg aagaaatcgg caaggtgcat cgggtgctgg tggaaggcag
      721 gagcaagcga tctgcagcgg acaactcagg cagatctgac tcgggtaaga agatcatttt
      781 tccggcagcc ggcacgaagc ccggggaaat tgtgcccgtc cgtattgaat cggtcacgtc
      841 cgcaaccatg cgcggaaccg tagttgctcc ggttttttcc tgaaattttt ccggagaaga
      901 tcgatactaa agatagcgtg aatcggctca tcagtattct cttattccta tggctggctc
      961 cattgctcgc cgaccgtgat gttcacgggg atatttcgtc tgcagaagtg tcgaggtacc
     1021 tggacgtgcg tctggacctg gatcgactgg ctgttcagcc ggggcagaag gtaatggtgc
     1081 gatatcgcgt cacaaatcag ggttatcgaa tctttcgcat ttttcctcat gaagacgcgc
     1141 gccgcacgtt tcagtttctg gtagaagaca aggccggaaa ggaagtggct ccgcgcgaaa
     1201 ggcccgaaga tgcatccgag caacctgtgc gtgacctggt ggggaatcga gtaaaagaaa
     1261 tccttcttca tcctggcgaa acgtttgaaa agacaatgga cctgaatgca atgtatgatc
     1321 tgtctccggg tcaggaatat agagtgtgtg cgtatttcgt gccagatcag gaatcagatc
     1381 ttgccattcg ctcaaaaaac tgggccaggg tgcgagtgag taagatggaa agagctccgg
     1441 ccgccagaga acaggacgca gcagtgagct ctgattcagg cctttctccg gaagaaacag
     1501 tctacctttt cctgtctgct gaaatcaaac acaactggcc tgcctatctc aagcatgtag
     1561 atttgcccag gttcgtggga tcgtatgatc gttttgcttt aaaatacgct caggcatccg
     1621 acctcgagag gccgggagtg cttcgcgaat tttctacgta tctgaccagg cgcgacggcg
     1681 acgacctgaa gcgttttcgc atcctgcgta cggatctgga tcgagacaag tccggggaaa
     1741 tccttcccac tggaaatgcc agggtcaccg ttcaagcgga tcgcgacacg gaagggtttc
     1801 gtgtccgcta cgaatatgtg tacactctca aaagagcaga gcaagagggg ttctggaaaa
     1861 tcatccatgt atctgctcga gtggttcgtc agggccagag ctgaaaaagg attgccacaa
     1921 tggttccaca cgttcaatct gagatgaggc cggcgcatga ctgagattct ttcccaggat
     1981 gaaatagatg ctctactttc cgcgatttcc acgggcgaag tggaaatgga agactacagc
     2041 aatgtaggcg aacaaaagaa agtaaagatc tatgactttc gcagacccga taagttctca
     2101 aaagaccaga ttcgtactct gcagatgatg catgaaacat ttgctcgtct gactacaacc
     2161 gcgctttccg ctcagttgcg tgcactcgtc ggtgtacacg ttggttccgt ggatcagctg
     2221 acgtacgaag aattcattcg atccattccg aatcctacca cacttg
//
LOCUS       DAORFF010000611         2249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_36763_length_2249_cov_4.710119, whole genome shotgun sequence.
ACCESSION   DAORFF010000611 DAORFF010000000
VERSION     DAORFF010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36763_length_2249_cov_4.710119"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="PKK76_18165"
     CDS             complement(<1..1169)
                     /locus_tag="PKK76_18165"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNL70459.1"
                     /translation="MARLPLLHSSVGVPYPANPRKEQRRVRKKSLPFARPDISQRERD
                     AVDRVLRSGWLTSGPEVHAFEEEFARSVGADHAIAVNSATAGLHLGLQVLGIGKGDLV
                     VLPSITFTATAEAVLYTGAKPAILDVGRSDYLLNAEVILKFIQDRCETREGKIFEKAS
                     GLRLKAIMPVHLGGRPCDLDAIQAIADKHDLHVVEDAAHAFPAKYKNRMIGNISDMTA
                     FSFYATKNLATGEGGMITTNNEKAAGRLRRLRLHGIKGQTWGRKRWKYDVVEQGYKYN
                     MTDLAAAMGRVQLARMNEMQERRMSIAARYREAFADIKGIQIVPDPDGSSQHLFTIEI
                     GKEAPVSRDRFVEEMYARGIGTSLHFIPLYRHTLYKEMGFKPADFPASEEIYKRIV"
     gene            1267..>2249
                     /locus_tag="PKK76_18170"
     CDS             1267..>2249
                     /locus_tag="PKK76_18170"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNL70460.1"
                     /translation="MKRLLSIIRPPRFEDVEKETSSQILFALIGIGFVMLTIYGVALA
                     LVIGFEHVAARRIQTVLTTLVILAFVTLVLRQGHLRLASQITVIGVGFTLALSGFQNR
                     GVLGIGFAGYTVLTVTASLLLGMRIGLLTALAASITGGVMLYFQTNHASHDDWLNLFS
                     SWAGESTLFFLCAGIVGFSSMKMRAAFNSTREEVIQRKAAETGYREAETRLNLALQAA
                     RMGLWEWDIASGKVTWSPVTEDLFRIPRGSFTQTYEAFLALIHPEDRDALKQSITLAL
                     GPPGSFSVQHRVLAVGQTLWMECHGMLLRSSSGQPERMVGTVVDITHRKAAA"
BASE COUNT          510 a          603 c          570 g          566 t
ORIGIN      
        1 acaatgcgtt tgtaaatctc ttcgctcgca ggaaaatccg caggcttaaa gcccatctct
       61 ttgtacagag tgtggcgata gagcggaatg aaatgaaggc tcgttccaat cccgcgggcg
      121 tacatctctt caacgaatcg gtctcgactg accggcgctt ccttaccaat ctcaattgta
      181 aagagatgtt gagaagaccc atcgggatcg ggtacgattt ggattccttt gatatccgcg
      241 aaggcctcgc gatagcgagc tgcaatcgac atacgtcttt cctgcatctc gttcatgcgc
      301 gctagctgga cacgacccat ggctgccgcg agatccgtca tattgtactt ataaccctgt
      361 tccaccacgt catatttcca gcgtttgcga ccccaggtct ggcctttgat gccgtggaga
      421 cgaagtctgc gcagtcttcc ggccgccttc tcattgttag tcgttatcat tccgccttcg
      481 cctgtggcca ggttcttggt ggcatagaag ctgaatgcgg tcatatcgct gatgttgcca
      541 atcatgcgat tcttgtattt tgctggaaat gcatgagccg catcttccac tacatgcaag
      601 tcatgcttat ccgcgatcgc ttgaatggca tccaggtcgc aaggtcttcc gcccagatgc
      661 acaggcatga tcgccttgag gcgaagaccg gaggcctttt caaaaatttt cccttcgcgc
      721 gtttcgcatc gatcctgaat gaatttcaga attacttcag cattcaacag ataatctgat
      781 cttccgacgt ccagaatcgc aggtttcgcg ccagtataga gaactgcttc tgcagtcgct
      841 gtaaatgtaa tcgaaggaag tactacgaga tctcctttcc caattccaag aacctggagt
      901 ccaagatgaa gaccggccgt ggccgaattt acggcgatcg catgatctgc accaacggat
      961 cgcgcgaatt cttcttcgaa agcgtgaact tcaggacctg aagtgagcca tccggatctc
     1021 agtacgcgat cgacggcgtc cctttcgcgc tgcgaaatgt ctggacgcgc aaatggaaga
     1081 ctcttctttc gcacacgtcg ctgctctttc ctgggatttg cggggtatgg aacaccgacg
     1141 gaagaatgca aaagtggaag tcgcgccata cattttatat cggcaaattg gaaaattcat
     1201 gaagggaatt gcaattgcaa cgagctggtc cagggtggac aaacggaggg ccgaaaggca
     1261 atttatttga agcgcttact ctctattata aggccaccgc gcttcgagga cgtagaaaaa
     1321 gaaacgtcct ctcagatcct ctttgctctc atcggaattg gcttcgtgat gctgaccatc
     1381 tatggcgtcg ccctggccct ggtgatcggc tttgagcacg ttgctgcacg cagaattcaa
     1441 acggttctca ctactctggt aattctcgca tttgtgactc tggttctcag gcagggacat
     1501 ttgcgcctcg ccagtcaaat cactgtcatt ggagtaggat tcacactggc actgtcgggc
     1561 ttccaaaacc ggggagtact tggcattggc tttgccggct atacggtcct cactgtgacc
     1621 gcatctctcc ttctgggaat gagaatcggt cttttgaccg cactggccgc gagcatcacc
     1681 ggaggagtga tgctctactt tcaaacgaat catgcatccc acgacgactg gctgaatctt
     1741 ttttcttcgt gggccggaga gagcactcta ttcttcctct gcgctggtat tgtcgggttc
     1801 agctccatga agatgcgagc tgccttcaat agtacccgcg aagaggtcat tcagagaaag
     1861 gccgcagaaa ccggataccg cgaggcggaa acacgactaa acctggcgct ccaggcagcc
     1921 agaatgggtc tctgggaatg ggacattgct tcaggcaagg taacgtggtc tcccgttacg
     1981 gaagatctct tccgcattcc gcgcggatcc tttactcaaa catacgaagc ctttctcgca
     2041 ctcattcacc ccgaagatcg cgacgcgctg aagcaatcca tcactctggc cctcgggccg
     2101 cccggttctt tttctgttca acaccgtgtg ctcgccgtcg ggcaaacgtt gtggatggaa
     2161 tgtcatggca tgctgctacg atcttcgagc ggtcagccag aaagaatggt tgggactgtc
     2221 gtagatatca ctcatcgcaa ggcagcagc
//
LOCUS       DAORFF010000612         2241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_36993_length_2241_cov_4.169716, whole genome shotgun sequence.
ACCESSION   DAORFF010000612 DAORFF010000000
VERSION     DAORFF010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36993_length_2241_cov_4.169716"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..759
                     /locus_tag="PKK76_18175"
     CDS             91..759
                     /locus_tag="PKK76_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HNL70461.1"
                     /translation="MSEEHKNLTFYEKLSEKIESKSLKFILFTTLAVLIGGLVEIPPF
                     FLMGSVQKIESVKPYNALELAGRDAYQQEGCFYCHTQVIRPFKWETDRWDRNREYGPE
                     PFSKAGEFVYEHPFLWGSKRTGPDLSHEASLNNNASWHRAHLMNPRETTPGSVMPAYP
                     WLFESKLDAAEVKSHMRALQTVGVPYTQTDMDAADELVKGKTQGDALIAYILKLGRDT
                     MVVK"
     gene            768..959
                     /locus_tag="PKK76_18180"
     CDS             768..959
                     /locus_tag="PKK76_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HNL70462.1"
                     /translation="MEELIGIYKGLRMPLLGVVLILLAAYVYWPSRKKDLEQARFNML
                     DDDVDHDLSKKFTSKPDKQ"
     gene            971..1507
                     /locus_tag="PKK76_18185"
     CDS             971..1507
                     /locus_tag="PKK76_18185"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNL70463.1"
                     /translation="MSFNEYRDDDEIKESRGWLPTWWMVLFWGAIAFSVVYGVYMHGV
                     EGWSMAKQYREEIEAFEKAHPEKLVALNDDGTNPYRTDAAAIERGKKTFESICAACHK
                     VDMTGLVGPNLTDTTWLHGNTDKELYAIVMNGVPQEKLKQNPPKGPMPAHKNSLGSAK
                     VLEVLAYVASKNPSLKAK"
     gene            1526..>2241
                     /locus_tag="PKK76_18190"
     CDS             1526..>2241
                     /locus_tag="PKK76_18190"
                     /inference="COORDINATES: protein motif:HMM:NF025121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNL70464.1"
                     /translation="MVVARPVQGKHRTIKNRINVVLLILFLVLPFLRFGGNPFVLLDI
                     PGRKFHVFGLEIWPHEFYFLHLILIMSGLSLFFFTALLGRIWCGYGCPQTIFMDFYDW
                     GAKLLVGSEYGKRPLNTSQKIRQYLAFFILAIFFSFIFLAYFVPYEEIASDLMHARFF
                     AGPDTLAPAAWVVFMVMSVGFAVFNAGYFRENACKLVCPYGRFQTALLDTHSPIVSYD
                     VKRGEPRREGKQKLFEHGGD"
BASE COUNT          573 a          534 c          574 g          560 t
ORIGIN      
        1 tacaacttga ttcaaacgat tcgtcgcaag ggtcagggct ttgtgccagt agatctcaga
       61 gaagcagcga gcaagtgagg agtatgaact atgtcagaag aacataagaa tttaacattt
      121 tacgagaagc tttctgagaa gatcgaaagc aaaagtctca aattcattct cttcacaacc
      181 ctggccgttc tgattggcgg tctggtggag atccctcctt tcttcctgat gggaagcgtt
      241 cagaagatcg aaagtgtgaa gccttacaat gctctcgaac tggcaggccg cgacgcctat
      301 cagcaagagg gttgcttcta ctgtcacaca caggtcattc gcccattcaa atgggagaca
      361 gaccgctggg acagaaaccg cgagtacgga ccggagccat tctccaaagc aggggagttc
      421 gtttacgaac atccattcct ctggggatcc aaaagaaccg ggcctgacct ttcgcacgaa
      481 gcaagcctga acaacaatgc gtcctggcac agagctcacc tgatgaatcc tcgtgaaaca
      541 acacctggtt ctgtgatgcc tgcgtatccg tggttgtttg aatccaaact ggatgcggca
      601 gaagtgaagt ctcacatgcg cgctttgcag acagtgggag ttccttacac ccagactgat
      661 atggacgccg ctgacgagct tgtcaaaggc aagactcagg gtgatgcgct catcgcgtac
      721 attctgaaac ttggccgcga tacgatggtt gtcaaatagg caaggcaatg gaagagctca
      781 ttggaattta taagggtttg aggatgccgc ttctgggcgt tgtcctcatt cttcttgccg
      841 cctatgtata ctggccttcg cgcaagaaag atcttgagca agccagattc aacatgctgg
      901 acgatgatgt ggatcacgat ctctcgaaga aattcacatc aaaaccggac aagcagtaaa
      961 ggagcaatgt atgtcgttta acgaatacag agatgatgat gaaatcaagg aaagccgcgg
     1021 ctggcttcct acctggtgga tggtgctttt ctggggtgcc attgcattct ccgttgtgta
     1081 tggtgtgtac atgcacggag tagaaggttg gagcatggca aagcaatacc gtgaagagat
     1141 tgaagccttc gaaaaagcgc atcctgaaaa actggtcgcg ctcaatgatg atggaaccaa
     1201 tccctatcgc acggatgcag ctgccatcga acgcggaaag aaaacgttcg aaagtatctg
     1261 cgctgcctgc cacaaagtag acatgaccgg tctggttggt ccaaacctga cggacacaac
     1321 ctggcttcat ggaaacacag acaaggaact gtacgcgatc gtaatgaacg gcgtacctca
     1381 agaaaaactg aaacagaatc cacccaaagg cccaatgcct gctcataaga acagccttgg
     1441 atccgcaaag gtgcttgagg tgctcgcata cgtcgcaagc aagaatcctt ctttgaaggc
     1501 caaatagggt aagggagaaa atatcatggt agtagcacgt ccggttcagg gaaaacacag
     1561 aacgattaag aatcgcatca atgtggttct tttgattttg ttccttgttc ttccgtttct
     1621 tcgctttggg ggaaatccgt tcgtgctgct ggacattcct ggcagaaagt tccacgtctt
     1681 cggactggaa atctggcccc atgaattcta tttcttgcac ttgattctaa ttatgtccgg
     1741 gctgagtttg tttttcttca cagctctgct cggacgtatc tggtgcggtt acggctgccc
     1801 gcagactatc tttatggatt tttacgattg gggcgcgaag cttctggtcg ggtctgaata
     1861 tggaaagcgt ccgctgaata cttcgcagaa gattagacag tatctcgctt ttttcattct
     1921 cgcaattttc ttctccttca ttttcctcgc atactttgtt ccatatgaag agatcgcatc
     1981 cgatctcatg catgccaggt tcttcgcagg cccggatact ctggccccgg ccgcctgggt
     2041 agtgtttatg gttatgtctg ttggcttcgc ggtctttaac gccggttact ttcgcgaaaa
     2101 cgcatgcaag ctggtctgtc cctacggacg ctttcaaact gcacttctgg atactcactc
     2161 accgatcgtc agttatgacg tgaagcgcgg cgaaccgcgc cgcgaaggca agcagaagct
     2221 atttgaacac ggcggggatt g
//
LOCUS       DAORFF010000613         2235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_37178_length_2235_cov_3.100917, whole genome shotgun sequence.
ACCESSION   DAORFF010000613 DAORFF010000000
VERSION     DAORFF010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37178_length_2235_cov_3.100917"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKK76_18195"
     CDS             <1..380
                     /locus_tag="PKK76_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70465.1"
                     /translation="TEMANRGGGILISQVLLPFVTGLDLVGIYIGDCLGEKRDSHVSA
                     AENFARIDFVTLEAGRIYGGLAEAPNESGILAVVPFVKPGQRLPHIGNGAQRHMMMIS
                     TERDRTLVEEMKLKLKERVLYNG"
     gene            373..687
                     /locus_tag="PKK76_18200"
     CDS             373..687
                     /locus_tag="PKK76_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70466.1"
                     /translation="MGKLKSFRVADDFALFYQENGFAVIDDLVSRKDAEGIRSEFLDI
                     CDHLGRKYAADFKSNDPVKRAHAIFSLGGDFRRNIEHFSSSCAAPHPWPRAMDLRISF
                     AR"
     gene            684..1040
                     /locus_tag="PKK76_18205"
     CDS             684..1040
                     /locus_tag="PKK76_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70467.1"
                     /translation="MNLKYPILRNQAIRIDFAEEPQYLQGIHQDVRGMRSANCLNFWI
                     ALREVNESKGTLSVFQEVIGTVRAYQIFNRVIFYHDPQVLKYCFTLTPQLAMKHDYLP
                     IPQREAMISLSRQSNS"
     gene            1058..1888
                     /locus_tag="PKK76_18210"
     CDS             1058..1888
                     /locus_tag="PKK76_18210"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNL70468.1"
                     /translation="MHFHQVMENDLHSATQTEDAARSLKTESTMDVSDYIECIERNAD
                     EWRKTCQSANFMFFNHSLFEGTTLSSVVQRIQAQFPGSACTALMNVEKPARLEVLRNL
                     KSIGFCAIKFHPYSQKIESQHLDCILEICGHAQTLGMPIFICTSFGTAKMHDHDGIGL
                     ACKIADRISGVPIVLLHAGAIRCFEAFLLAEDRLNVFLETSFSLDYLEGSRYIDDFMF
                     IFKRLGSERILYGSDSPFVRMDSAKEALERRLRGYGFSGAELQNIFHDNAARMFARKD
                     "
     gene            1890..>2235
                     /locus_tag="PKK76_18215"
     CDS             1890..>2235
                     /locus_tag="PKK76_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HNL70469.1"
                     /translation="MNGVLFLTEAGPEIGLGHVTRMRAVEAYLAERNVETALVVRDIS
                     SPDSCEWLADFSWLPDCRGFDCVVVDSYLADRSKYEILRREFSRVVAIDDYNRIVYPA
                     DLIINPNVYFSSV"
BASE COUNT          528 a          524 c          594 g          589 t
ORIGIN      
        1 tgacagagat ggcaaatcga gggggcggca tcctgatttc gcaggtcctg cttccattcg
       61 tcacagggct cgatctggtg ggtatctaca ttggagactg ccttggtgag aaaagggatt
      121 cgcacgtgtc cgcagccgag aattttgcgc gcatcgattt tgtgactctc gaagccggca
      181 gaatttacgg aggcctcgcg gaagctccaa acgaatcagg aatcctggca gtcgtgccgt
      241 ttgtaaagcc tggccagcgc ttaccgcata tcgggaatgg cgcgcagcgt catatgatga
      301 tgatttcgac ggaaagagac aggacgcttg tggaagaaat gaagctaaaa ttaaaggaac
      361 gggtattgta caatgggtaa attgaaaagc tttcgcgtcg cagatgattt cgccttattt
      421 tatcaggaga acgggtttgc tgtcatagac gatctggtat ccagaaaaga tgcggaagga
      481 attcgttcgg aattcctgga tatctgcgat catctgggca ggaagtatgc tgcagatttc
      541 aagagcaatg atcctgtgaa acgcgctcac gcaatctttt ctctgggagg agactttcgc
      601 aggaacattg agcacttttc cagcagctgc gcagcaccac atccctggcc gcgagcgatg
      661 gatttacgaa tatctttcgc aagatgaatt tgaaatatcc gatcctgcga aaccaggcaa
      721 tccgcatcga ctttgctgag gaaccccagt atctgcaggg aattcatcag gatgtccgcg
      781 gaatgcgttc tgccaattgc ttgaatttct ggatcgcatt gcgggaagta aacgagtcga
      841 agggcactct ctctgtgttc caggaagtca tcgggacggt gcgcgcatac caaatcttca
      901 accgggtcat cttttatcac gaccctcagg ttctgaagta ttgtttcact ttaacgccgc
      961 agcttgcgat gaagcatgac tatctgccga tcccgcagcg tgaagcgatg atatcacttt
     1021 ccaggcagtc taactcttga atgtcttcga cgtaaatgtg cacttccatc aggtgatgga
     1081 aaacgacctc cacagtgcga cccagacaga ggatgcggcg cgcagcctga aaacagagtc
     1141 gacgatggac gtctctgact atatcgaatg cattgagcgg aatgcggatg aatggagaaa
     1201 aacatgccag agcgccaact tcatgttttt caaccacagc ctgtttgagg ggacaacgct
     1261 ttcttctgtc gttcaaagaa tccaggctca gtttccgggg tctgcctgca ccgctctcat
     1321 gaacgtggag aagcctgccc gccttgaagt tcttcggaat ctaaaatcga ttggcttttg
     1381 cgccatcaag tttcatccct attctcagaa gattgagagc cagcatctcg attgcatcct
     1441 tgaaatttgc ggacatgcgc agacactcgg aatgccgatc ttcatctgca caagttttgg
     1501 gacagcgaag atgcacgatc atgacggcat cggtcttgcg tgcaagattg cggatcgcat
     1561 atccggagtt ccgattgttc tccttcacgc cggcgcgatt cgatgctttg aagccttcct
     1621 gcttgcagag gacagattga atgtctttct cgagacgtcc ttctccctgg attacctgga
     1681 aggttcgcgc tacatcgatg atttcatgtt tatattcaag cgtcttggct ctgagcggat
     1741 tttgtatgga tctgattctc ctttcgtgcg aatggactct gctaaagaag cgctggaacg
     1801 cagactgaga ggctacggtt tttcgggagc agagctgcag aatatttttc atgacaatgc
     1861 tgcccgtatg tttgcccgca aagattagta tgaacggtgt tctttttctg accgaggccg
     1921 gaccggaaat tggactgggg catgtgacca gaatgcgggc tgtcgaagca tatcttgcag
     1981 agcgcaacgt agagactgct ctcgtggtgc gagatatcag ctctcctgat tcttgtgagt
     2041 ggcttgctga cttctcatgg ctgccggatt gccgcggctt tgactgcgtt gttgtagatt
     2101 cgtatcttgc ggaccggagc aaatatgaaa ttcttcgcag agagttttcg cgtgtagtcg
     2161 ctatcgatga ctacaatcgt attgtgtatc ccgcggatct gatcatcaat ccgaatgtct
     2221 atttttcgtc tgtgg
//
LOCUS       DAORFF010000614         2229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_37381_length_2229_cov_3.576357, whole genome shotgun sequence.
ACCESSION   DAORFF010000614 DAORFF010000000
VERSION     DAORFF010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37381_length_2229_cov_3.576357"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKK76_18220"
     CDS             complement(<1..246)
                     /locus_tag="PKK76_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70470.1"
                     /translation="MTWTLYDERLRNPWLALAIDEAVCHFFAGKEFDSLIRVWRNPSA
                     ICFGRTCDASLNAHGESLLNLDPGFSRSHFENKTVLLR"
     gene            complement(243..665)
                     /locus_tag="PKK76_18225"
     CDS             complement(243..665)
                     /locus_tag="PKK76_18225"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNL70471.1"
                     /translation="MAEPETRNRIRLQFPSQPRYVATIRDTIYRFCLQHGFARAAAFD
                     VKVIAGEALTNIIQHAYGGGADHPIFIEMLFFKEYGEFRFKDLGKQVPITNQMKKDLT
                     DYREGGLGLYLIDQLSDFHVYDQSEKVGTTLIVKKKMA"
     gene            743..1081
                     /locus_tag="PKK76_18230"
     CDS             743..1081
                     /locus_tag="PKK76_18230"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL70472.1"
                     /translation="MASYAINNNYKIKVLEISGRFTIENRDEFQAFIKENVDMSAPGT
                     CIDATDLGYIDSSGIGELLRLKMSAAKTNHVIYVFGLSDAILKMFKMSQLNHVFVIMT
                     KEEFESQFKA"
     gene            complement(1104..1658)
                     /locus_tag="PKK76_18235"
     CDS             complement(1104..1658)
                     /locus_tag="PKK76_18235"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNL70473.1"
                     /translation="MKRIPIALRVLIVWFLVHTSFILYDGFSDSVEPGHTAIVFGNKV
                     EETGLPSERLERRLIRALDLYNQGTANHLFVSGGVGKEGFDEALIMKQYLVAHGVPEE
                     AITQDSEGVDTRATARNFAVAGGGPGAIVVTDYFHISRAKLALRNVGLPTRFSAHAPF
                     RFHIKELYYVPREFFGYYFYLIRR"
     gene            complement(1655..1882)
                     /locus_tag="PKK76_18240"
     CDS             complement(1655..1882)
                     /locus_tag="PKK76_18240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70474.1"
                     /translation="MASKILKVVSPMPAASHSAGSSQKEKQAIAKVKVSVDKAKIARK
                     IARSQVPVPAPRAHASKTKYTRKVKHRKQPE"
     gene            complement(1904..>2229)
                     /locus_tag="PKK76_18245"
     CDS             complement(1904..>2229)
                     /locus_tag="PKK76_18245"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNL70475.1"
                     /translation="KTSERSLYDESFRKANDLGFDDAIFLNETGEVAETAICNLMVKK
                     GEVWVTPEPSAGALPGIMMRECMRRFPGRIVFGTVLAEDLKGRVLVCNSVRGIMRARI
                     FDSTK"
BASE COUNT          554 a          575 c          546 g          554 t
ORIGIN      
        1 gcgaagcagg actgtcttgt tctcaaagtg agaacgacta aagcccgggt caaggttaag
       61 cagagattcc ccgtgcgcgt tgagagaagc atcgcacgtt ctcccaaaac atatcgcaga
      121 tggatttctc caaacgcgaa tcaggctatc gaattccttt ccagcaaaga aatggcagac
      181 tgcttcatca atggccagtg ccaaccaggg atttcgcaga cgctcgtcgt ataacgtcca
      241 ggtcatgcca tttttttctt aacgatgagc gtggttccaa ctttctccga ctgatcgtaa
      301 acatgaaagt cggaaagctg atcaattaga tacaatccca gaccaccttc gcggtaatcg
      361 gtgagatctt tcttcatctg gttggtaatc ggaacctgtt tgccaagatc cttgaatctg
      421 aattcgccat attctttgaa gaagagcatc tcgatgaaaa tcggatggtc cgcgccgccg
      481 ccatacgcat gctgtataat attggtgagc gcttccccgg caatgacttt gacatcgaaa
      541 gcggcggcac gcgcgaatcc gtgctgtaag caaaagcgat agatggtgtc ccggatggtc
      601 gcgacgtacc tgggttgaga agggaattgc aggcgaatgc gatttcgagt ctccggctcg
      661 gccatgggat agtctgtcct cccggcccgc cagggcaaga taaaagtgtt tgacagggcc
      721 tgtttgaaat ggacgcttcg gcgtggcatc ttacgcgatc aataacaact acaagatcaa
      781 agtcctcgag atttcaggca gattcacaat agaaaacagg gacgaattcc aggcgttcat
      841 caaagagaac gtggacatga gcgctcctgg cacctgcatc gacgccacgg atcttggcta
      901 cattgactca tcgggcattg gagaactact cagactcaaa atgtctgcag caaagacaaa
      961 tcacgtgatc tacgtgtttg ggctttcaga tgcaatcctc aagatgttca agatgtctca
     1021 gctcaatcac gtgttcgtga tcatgacaaa agaagaattc gaaagccagt tcaaagcttg
     1081 aggccaggtc cgcgcccggc aagtcagcgt cgtattaaat agaagtaata gccaaagaat
     1141 tcgcgcggca cgtagtagag ttccttgatg tgaaatcgaa acggtgcatg cgcggagaac
     1201 ctcgtgggca ggcccacatt tcttaacgcc agctttgccc tcgaaatgtg aaagtagtcc
     1261 gtaaccacaa tggctcctgg tccaccaccg gcgacggcga agtttcgtgc agtggctcga
     1321 gtatccacac cttcgctatc ttgcgtgatg gcttcttccg gaactccgtg agcgaccaga
     1381 tactgcttca tgatcagtgc ttcatcgaaa ccttctttgc ccactccgcc gctcacgaaa
     1441 agatgattgg ccgttccttg attgtagaga tcgagagcgc gaatgagtct gcgctccagc
     1501 ctctctgaag ggagtccggt ttcttccact ttgtttccga agacaatagc tgtatgaccg
     1561 ggttctaccg agtccgagaa accatcataa agaatgaatg aagtatgaac cagaaaccag
     1621 acgattagaa ctctcagcgc gatgggaatt cttttcattc aggctgcttc ctatgcttta
     1681 ctttgcgagt gtattttgtt ttcgaggcgt gcgctctggg agcaggaaca ggcacctgtg
     1741 atcttgcaat tttgcgcgcg atctttgcct tatccacgct cactttgacc ttcgcgatgg
     1801 cctgcttctc cttctgggaa gatccggcag aatgcgatgc ggccggcatg ggcgacacta
     1861 ctttcagaat cttgctggcc atagttctac ttcgtccaca gaatcatttc gtcgagtcaa
     1921 atattcgagc gcgcatgatg cctcgcacag aattgcatac gaggacccga ccttttagat
     1981 cttcggcaag tacagttcca aacacaattc tgcctggaaa gcgtcgcatg cattcgcgca
     2041 tcatgattcc agggagcgct cccgcagaag gctccggcgt gacccagacc tcccctttct
     2101 tcaccatcag gttgcaaatg gcagtctctg ccacctctcc tgtttcgttc aggaagattg
     2161 cgtcatcgaa cccgagatca tttgctttgc gaaacgattc atcatacaaa cttctttcgc
     2221 ttgttttgt
//
LOCUS       DAORFF010000615         2222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_37596_length_2222_cov_3.247808, whole genome shotgun sequence.
ACCESSION   DAORFF010000615 DAORFF010000000
VERSION     DAORFF010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37596_length_2222_cov_3.247808"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="PKK76_18250"
     CDS             complement(<1..1387)
                     /locus_tag="PKK76_18250"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL70476.1"
                     /translation="MRLARAVFVLPLFLMFAACAPAGEGVKHGTVSLSPSTITSLRGT
                     WTLCLSDSDCREFPVPGGWIPHGLRITSATYKLNVKSTPGMKIGLVIPRTLAAYEVYW
                     NHSLAGKSGKMEDAASSYEALTKRLFLFPESAAGEDLLEIRVAGIGEVGGVVQPEIYA
                     GPAGPVSDLYFARRTVSSILAGVFLFAGFSHLLFYFSRPKQFPYLYYSIACLLSTVAM
                     IAAEGITYLITPSGVFNTISLTTVLALMPPATLLFVEGFFGRSQSAITYVVIAAGLAL
                     CAVLWTGLFSIQAFVFVVRYFLPVLMVFMLSSYFYIVYLVIRALISGQNGAVIVLSSL
                     LLFILGFLNDFAVNRGYFRSFSLSGEGYLCFITGMSIALARQFALFQREHDQLHRQLE
                     SSENNYRSLVEGSGQMIFALDLEMRVTSANQACRSLLGMDPRKLIGKPFSQLVHASGH
                     LESWLAGISTLM"
     gene            complement(1389..>2222)
                     /locus_tag="PKK76_18255"
     CDS             complement(1389..>2222)
                     /locus_tag="PKK76_18255"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNL70477.1"
                     /translation="GCVGTNALLLKMRKYKKLNHLSPMLGKKWCGNGDLEGTVLFSDR
                     EHPLNNGPVITAAVQHRFKDYPDGFGHGLVLQEAGIPNFIAWFIAGRLPSIGFFYKLF
                     VVPFSALWNLIRRVFTKRARSQINPGNYIASLMDQDSYLRKTMVLLGMGRDRSDGEVT
                     LNDDDEPIIRWRMDHSRLHYDRVRREMEKMAHALGGKFMENPISYMDKIIAVHPLGGC
                     VMADTAEGGVVDTRGEVFGHPGLFVVDASVLPTSVGPNPSLTITALAEYFASKWPKKG
                     A"
BASE COUNT          630 a          595 c          538 g          459 t
ORIGIN      
        1 ccatgagagt tgaaatcccc gcaagccagc tttccagatg tccggacgcg tgaaccagct
       61 gagagaacgg ctttccaatg agcttgcggg gatccatccc gagaagagat ctgcatgcct
      121 gattggcaga tgtgacgcgc atctccagat ccagggcgaa gatcatttga cccgatccct
      181 ctacgagact gcgataattg ttctcagaac tttccagttg cctgtggagt tggtcgtgct
      241 cccgctggaa gagcgcaaac tgacgggcca gcgcgataga catgccagtg atgaagcaca
      301 gatatccttc tcccgagaga gaaaagctgc gaaagtaacc tctgttcaca gcaaagtcat
      361 tcaaaaaacc aagaataaaa agaagcaaac tcgaaagaac aatcacggcc ccattctgtc
      421 cggaaatgag agctcgaatt actaaataaa ctatataaaa ataggacgag agcataaaga
      481 ccatcagaac tggcagaaag taacgaacca caaacacaaa agcctgaata gaaaacagcc
      541 cggtccacag aaccgcgcac agtgcaaggc ccgcggcgat cacaacataa gtgatggcac
      601 tctgcgaacg tccaaagaat ccttctacga agagcaaggt agcgggcggc atcaatgcaa
      661 gcactgttgt aagcgagatc gtattgaaaa cgccagacgg agtgatgaga tacgtgatcc
      721 cttccgccgc aatcatggcc acggtggaaa gaagacaggc aatcgaatag tatagatatg
      781 ggaactgttt tggacgactg aagtagaaca ataagtgact gaagcctgca aacaaaaaca
      841 caccggcaag aatcgaagag acagtccgtc gcgcgaaata cagatctgac acaggccccg
      901 cgggcccggc ataaatctca ggctgcacca ctccgcccac ttctccaatt ccggccacgc
      961 ggatttccag tagatcctcg ccggccgcag attccggaaa aaggaacaac cgtttggtga
     1021 gcgcttcata ggaagaggcc gcatcctcca ttttcccgga tttgcccgca agagaatggt
     1081 tccagtacac ctcataagca gcgagcgttc gcggtatcac gaggccgatc ttcattcccg
     1141 gcgttgactt cacattgagc ttgtatgtag cactggttat gcgaagacca tggggaatcc
     1201 aaccgccagg aacgggaaac tccctgcaat ctgaatccga aagacacaga gtccaggttc
     1261 ctcgcaaaga tgtaatggta gacggcgaaa gcgacacagt gccgtgtttt actccctctc
     1321 ctgcaggcgc acacgcggca aacatcagga acagaggcaa tacaaaaacc gctcgcgcca
     1381 gtcgcaaatc acgccccttt cttcggccac ttgcttgcga agtactctgc cagagcagtg
     1441 atggttaagg aaggattggg gccaacggaa gtgggcagta cacttgcatc gacaacaaag
     1501 agacctggat gcccgaacac ttctccacgc gtatccacga ctccaccttc cgccgtgtct
     1561 gccatcacgc atcctcccag aggatgaacc gcaatgatct tatccatgta agagattgga
     1621 ttttccataa attttccccc gagggcgtgg gccatttttt ccatttcgcg tctgacgcga
     1681 tcgtagtgaa gcctgctatg atccatccgc cagcgaatga ttggttcatc atcgtcgttt
     1741 aacgtcactt ccccatcaga acgatcacga cccattccca gaagaaccat tgtttttcgc
     1801 aaatagctgt cctggtccat gagagatgcg atgtagttgc ctggattgat ctgactccgc
     1861 gcacgtttcg taaaaacccg gcggatgaga ttccagagcg cagagaaagg aaccacgaac
     1921 aatttataga agaacccgat agaaggcaat ctacctgcga tgaaccaggc gatgaagttt
     1981 ggaattcccg cttcctgcaa aacaagcccg tgaccgaatc catccgggta atctttgaat
     2041 ctgtgctgaa cggctgcagt gatcacagga ccattgttca gagggtgctc tctgtctgaa
     2101 aatagaaccg ttccttctag atcgccattg ccgcaccatt tctttccgag catcgggcta
     2161 agatggttta gtttcttata ctttctcatt ttcagaagta gcgcgttggt cccaacgcag
     2221 cc
//
LOCUS       DAORFF010000616         2211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_37959_length_2211_cov_3.500928, whole genome shotgun sequence.
ACCESSION   DAORFF010000616 DAORFF010000000
VERSION     DAORFF010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37959_length_2211_cov_3.500928"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PKK76_18260"
     CDS             <1..667
                     /locus_tag="PKK76_18260"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNL70478.1"
                     /translation="QDALDSRKKHSPIASGRIGPAGAIAAIVILVGTSATLASFLPIT
                     FRIILSIYLIQNIFYTWLGKRILLIDVFMISLGFVFRVLAGAAALGIAASPWLLSCTL
                     FLSLFLGFFKRYYELRTSPPEIMIGGQYTAESLKNFLSITASLSVMNYAVYTLEGRYA
                     GEKLYWTIPLVVLGIFRYYTLMDRPELEDGNPSDVLLTDKFLIAVIGCWVLSCAALIL
                     HIA"
     gene            739..948
                     /locus_tag="PKK76_18265"
                     /pseudo
     CDS             739..948
                     /locus_tag="PKK76_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            1065..1922
                     /gene="lpxI"
                     /locus_tag="PKK76_18270"
     CDS             1065..1922
                     /gene="lpxI"
                     /locus_tag="PKK76_18270"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HNL70479.1"
                     /translation="MKRLGILAGRGELPWIAARAAVAAGEDVRVFCFTNEEPPAEFKS
                     ISIKVVLTKLYSSVFKNMKAHNVGKVIMIGKATRDILYDNPRFDLRALLLLARMTNQA
                     DTTIFDFASREFEKRGFHIIPQDTHLKHLYLPEGRYGPALKAREIDDISFGMIYAREI
                     NRLDLGQTVVAGQLSILAVECAEGTDQCIRRGGGLFRNKGAVVCKVAKTAHDPRFDLP
                     VTGLSTLQSMKESGCRILAIESGRTLVVHHAEFIRTAQKMGISIVSCNPDKSDPKTLT
                     KLCKKAVRV"
     gene            1919..>2211
                     /locus_tag="PKK76_18275"
     CDS             1919..>2211
                     /locus_tag="PKK76_18275"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNL70480.1"
                     /translation="MKTLRVSTATKKTRSKIAHEILSSGIHGPLLVVAGEHSGDLLGG
                     DVLSHLKKLGLKEFFGTGGETMKREGAEIIQDVESMAVMGFVDALRNYSRLHKL"
BASE COUNT          540 a          591 c          563 g          517 t
ORIGIN      
        1 ccaggatgcc ctggacagtc gcaagaaaca cagccccatt gcaagcggcc gcattgggcc
       61 ggcaggagcc atcgccgcca tcgtcatcct cgtaggcacc tccgccactc tcgcatcgtt
      121 tctccccatc actttcagaa tcattctctc tatttatctc attcagaata ttttctatac
      181 ctggcttggg aaaagaatcc tgctcataga tgtgttcatg atttctctcg ggtttgtttt
      241 tcgcgtgctg gccggtgccg cagcgctcgg aatagccgcc agcccctggc tactttcgtg
      301 cactttgttt ttgtctctct tccttggttt ctttaagaga tattacgaac ttcgcacatc
      361 tcctccggag atcatgatcg gtgggcagta cactgcggaa agcctgaaga actttctaag
      421 catcacggct tctcttagcg tgatgaacta tgcagtctac accctggaag gcaggtatgc
      481 cggtgagaaa ctctactgga cgattccact cgtagtgctt ggaatctttc gctactacac
      541 tttgatggat cgcccggagc tggaagacgg gaatccctct gacgtacttt tgacagataa
      601 attcctcatc gcagtgattg gctgctgggt actatcctgc gcggctctca tattgcacat
      661 cgcctgacgc ctggaactgc ccccgactga cagcccaaaa ctgcttgatc ggacggccat
      721 tgaaacgaaa cctggagcat gcctacatac tcctatcagt gccaggattg cggtcatgaa
      781 tacgacgcat ttcagtccat gaaggatgac ccagatacag tctgccccaa atgcggcgga
      841 agagtggaac gactcattgg tgccggagcc ggaatcgttt tcaaggggtc agggttttac
      901 gtaacggact ataagaaaac gtccagcagc acgagcgcgc caaagactgt agagaaaagc
      961 ggcggagaaa gtaagcccgc cggatcgagc gattcgtccg gaggttcttc cgcaagctcc
     1021 agccagactg gttccaccgg ttcaggttcg agcggttcgg gtacatgaaa agactcggca
     1081 ttcttgccgg acgcggcgag ctgccatgga ttgcggccag ggccgcggtg gcggcaggcg
     1141 aagatgtgcg cgtgttctgc ttcacaaacg aagaaccacc tgcagaattt aagtccattt
     1201 ccatcaaagt agttcttact aaactctatt catccgtttt caagaacatg aaagcgcata
     1261 acgtcggcaa ggtgatcatg atcggcaagg ccacgcgcga cattctatac gacaatccgc
     1321 gatttgacct gcgagcattg ttgcttctgg cacgcatgac caaccaggca gacacaacga
     1381 tctttgattt tgcctcgcgc gaatttgaaa aacgtggatt tcatattatt cctcaggata
     1441 ctcacctcaa gcatctgtat cttccggaag gcagatatgg tcccgccctc aaggcgcgcg
     1501 aaattgacga catttctttt ggcatgatct acgctcgtga gatcaaccga ctggacctgg
     1561 gacagacagt ggtggccggg caactctcca tccttgcggt cgaatgcgcg gaaggaacgg
     1621 atcaatgcat tcgtcgcggc ggcggtctat ttagaaacaa gggtgcagtc gtctgcaaag
     1681 tggcaaagac ggcccatgat ccacggttcg atcttccagt cacgggcctt tccacccttc
     1741 agtccatgaa ggaatcagga tgtagaatcc ttgccattga atccggacgc actcttgtag
     1801 tccaccacgc cgagttcatt cgcaccgcgc agaaaatggg aatcagcatt gtgtcgtgca
     1861 acccggataa atcagatcca aagactctga caaaattgtg caagaaggca gtcagagtat
     1921 gaagactctc cgcgtgtcca cagcaacaaa gaagaccaga agcaaaatcg cccatgaaat
     1981 actgtcctct ggaattcacg gccctctgct cgtagtcgca ggagaacatt ccggtgatct
     2041 tctgggcggg gacgttcttt cgcatttgaa gaaactgggt ctcaaagaat tctttggaac
     2101 tggcggggaa accatgaagc gcgaaggcgc agagatcatc caggatgtag agtccatggc
     2161 ggtcatggga ttcgtggatg cacttcgcaa ttactccaga ctgcacaaac t
//
LOCUS       DAORFF010000617         2190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_38631_length_2190_cov_3.325527, whole genome shotgun sequence.
ACCESSION   DAORFF010000617 DAORFF010000000
VERSION     DAORFF010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38631_length_2190_cov_3.325527"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PKK76_18280"
     CDS             complement(<1..456)
                     /locus_tag="PKK76_18280"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNL70481.1"
                     /translation="MGELKDFLASFLMPQRLARIRDVLSKRTNHITVVLEDIYDPHNA
                     SAVLRSCDAMGIQTVHIIQKRHTFQVKEGVSMGTGRWLDLRMHKETSECFDELRKNGY
                     SIASASPPLSGTVLLPDFQSPGKTAIVLGSEKDGLSPFARENSDCLITIP"
     gene            542..934
                     /locus_tag="PKK76_18285"
     CDS             542..934
                     /locus_tag="PKK76_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HNL70482.1"
                     /translation="MRVKISELKIGNRIRTNPGDLTALRDSMRRLGLLQPILIDSKYN
                     LIAGFRRLESAKMLGWESIEARLIETRDKKERLLIEADENTTRRDFSPEEMGRVDKLL
                     LRYSKGGIFWKFIAWLMDLFEWIFRRRS"
     gene            complement(946..2013)
                     /gene="rlmN"
                     /locus_tag="PKK76_18290"
     CDS             complement(946..2013)
                     /gene="rlmN"
                     /locus_tag="PKK76_18290"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNL70483.1"
                     /translation="MSEETPILKGALIEELTEFFKNIGEPGYRGKQAFIRINRFQART
                     LSEFTEFPLALREKLEAMGAFPDLPIIQTSKDADGTEKAVFAAGTDRRDEETKKIEAV
                     WIVSEGRRTACISSQVGCTLNCAFCATGTMKFRGNLKAWQIVDQVYGLMRHRGSSMAA
                     DDPERLTNVVFMGMGEPFYNYDQALRAAHLLHNPHGLNLGARHITISTAGVVPGIERF
                     TSEGQPFNLAISLNHPDPEGRRDIMDITDRFSLPDLLAAVRAYTKASGKPVMFEFVMI
                     PDKNMGKENVEKLVAIAKSVNCKINLIPLNTNLHNWRRPTDEEMVEFQNALRQEGILA
                     FNRGSPGRRVGGACGMLALTG"
BASE COUNT          497 a          573 c          546 g          574 t
ORIGIN      
        1 gggaatcgta atcagacagt ccgagttctc gcgcgcgaac ggggaaagcc catctttttc
       61 agatccaagt acgatggccg ttttgccggg cgactgaaaa tcggggagca gcactgtgcc
      121 cgaaagagga ggagaagcgc ttgcgatgga gtacccattc ttgcgcaatt cgtcaaagca
      181 ctctgaagtt tctttgtgca ttcgcagatc caaccaccgg cctgtcccca tcgacactcc
      241 ttccttcacc tggaaagtat gcctcttttg aatgatgtgg accgtttgaa ttcccatggc
      301 gtcgcagctt cgtaacaccg cgcttgcatt gtgcggatcg tatatatctt cgagcactac
      361 tgtgatatga ttggtgcgtt ttgatagaac gtcccggatg cgcgccagtc gctgcggcat
      421 taagaaggat gccagaaagt ctttcaactc tcccataatt tgtcacatat agcatccgct
      481 tgcggcatga aaaggcgata aaacggcttg cgctcggaag gaactccgcc aggatcacac
      541 catgcgggtg aaaatctctg aactgaaaat cggtaaccgg attcgtacga atcctggtga
      601 tctgacagcg ctcagagatt ctatgcgtag actcgggctg ctgcagccaa ttctgatcga
      661 ctcaaagtac aatttgattg caggcttcag aagattggaa agcgcgaaga tgcttggctg
      721 ggaatctatt gaagcgagac tcattgaaac ccgggataag aaagaacgcc tgctcattga
      781 agcagatgag aataccactc gccgcgactt ttcgccggaa gagatgggcc gcgtagacaa
      841 gctccttctt cgatacagca aaggcggcat cttctggaaa ttcattgcat ggctgatgga
      901 tttgttcgag tggatctttc gccgtcgcag ctgattgggg cgcgttcaac cagtcaacgc
      961 cagcataccg cacgcaccac ccacacgcct tccgggggag ccgcgattga atgcgagaat
     1021 cccttcctgg cgtagcgcgt tctgaaattc taccatctct tcatccgtag gtcttcgcca
     1081 gttgtggaga tttgtgttga gtggaatcaa attgattttg caattcacag atttcgcgat
     1141 cgccacgagt ttttctacat tctcttttcc catgttcttg tctggaatca tcacgaattc
     1201 aaacatcact ggcttaccgc ttgccttcgt gtatgccctt acagctgcca atagatcggg
     1261 aagagaaaat cgatctgtaa tgtccataat atcccgacgg ccttctggat ccgggtggtt
     1321 gagcgaaatg gcaagattga atggctggcc ttcgctcgtg aatctttcaa tccctggaac
     1381 tactcctgca gtagaaattg taatgtgccg cgcacccagg ttcaggccgt gaggattgtg
     1441 aagtagatgc gccgcgcgca atgcctgatc ataattgtag aagggctctc ccattcccat
     1501 gaacactaca ttggtcaatc tctctggatc gtctgctgcc atactcgagc cgcggtggcg
     1561 cattagaccg tacacctgat ctacaatttg ccaggctttg aggttgccgc gaaatttcat
     1621 ggtgccagta gcacagaatg cgcagttgag tgtgcatcca acctgagacg aaatgcacgc
     1681 tgtgcgtctt ccctcggaga cgatccacac agcctctatt ttctttgtct cttcgtctct
     1741 gcgatcggtt ccggcggcaa agactgcttt ttcagttccg tccgcgtcct tgctcgtctg
     1801 gatgatgggc aaatcgggga acgcacccat ggcctctaac ttttcgcgga gtgccagcgg
     1861 gaactctgta aactcagata gagttcgcgc ctggaatcga ttgatccgaa tgaatgcctg
     1921 tttgccccgg tagcctggct ctcctatgtt tttgaagaat tcagttagct cttcgatcag
     1981 agcgcctttc aggataggag tttcttcgct catgcttcta gattcctctc agaagcactt
     2041 tctccgtgtt cctgatgacg atccctgctt tgtgcgcgga gagtcctgcc tgctggatgc
     2101 ttgcaacaac ggactctacc tgatccggac gcactgccat gatcatccct gcacccatat
     2161 tatatacgga atacatttcc tttctggcta
//
LOCUS       DAORFF010000618         2185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_38778_length_2185_cov_3.811268, whole genome shotgun sequence.
ACCESSION   DAORFF010000618 DAORFF010000000
VERSION     DAORFF010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38778_length_2185_cov_3.811268"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PKK76_18295"
     CDS             <1..588
                     /locus_tag="PKK76_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70484.1"
                     /translation="HFANPAFDLGRDDCEVVLEAGKKVYRIQETRKPTTFYSLLRFLE
                     FFRSNTEKLTQNLNLIEQHYDRVHKTKHENTGLRGLLRKQWTSDMIDNKHEIIAAALQ
                     KDTNVDSVLGKLEHLLAENLPVTTRWQYLLQPFMNEVINHINHNLRDGAFNLRMYYPV
                     RTMEDKFKPYYSNAMRYAVELIDAEKITSAFVKRL"
     gene            589..1107
                     /locus_tag="PKK76_18300"
     CDS             589..1107
                     /locus_tag="PKK76_18300"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNL70485.1"
                     /translation="MLNYIRKNWSSILLFAFVIGFVLYRKIPLWENERGLEGQSLPHG
                     SLRTLAGSEIDLSSRKEKVILVSFWATWCMPCRVEMPMLVSLRRELDGQSFEIFAVTD
                     EDPITVAHFIEEHPVNFPIVLDRGPLQQAFRIQVFPTLVQFKDGKLVSVSNGVDWFLK
                     FKIRHAVTGNYF"
     gene            1113..>2185
                     /gene="guaA"
                     /locus_tag="PKK76_18305"
     CDS             1113..>2185
                     /gene="guaA"
                     /locus_tag="PKK76_18305"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNL70486.1"
                     /translation="MSDNRKIGVVDFGGQYAHLIASRIRRLGALAQIADPETLTPSEA
                     RAQYSGLIYSGGPSSVYEPGAPASDPALLSAGLPVLGICYGHQLMMQQLGGEVRNSGH
                     HEFGRAILKVKSDVSVLRGENNSVSAVWMSHGDEVTRMAPGFVGIAHTDDCENAAVFN
                     AEKNLTGIQFHPEVQHTEHGDQYLLNFIRMCGLENSYRIGDFLSKEKEALARSIGDRS
                     VFFLLSGGVDSTVAFTLLSEILPADRLYGLFIDTGFMRKDEGIEVGTALRAHGIQLNI
                     EDARKRYATSLAGVVDPEKKRRIIGDLFLEIQADCVRRLNLDPEHWLLGQGTIYPDTI
                     ESGGTKKSHLIKTHHNRVPAIQR"
BASE COUNT          546 a          506 c          550 g          583 t
ORIGIN      
        1 cattttgcaa atcctgcatt cgatcttgga cgcgacgact gcgaagtagt gctggaagcg
       61 ggcaagaagg tgtacaggat tcaggaaact cgaaagccta ccacgttcta cagtctgctt
      121 cgctttcttg aattctttcg ctcgaacaca gagaagctta cccagaacct caatttgatt
      181 gagcagcatt acgatcgggt tcacaaaaca aagcacgaga acaccggtct tcgtggcctc
      241 ttgcgaaagc aatggaccag cgacatgatc gacaacaaac atgaaatcat agcggctgca
      301 cttcagaaag atacaaatgt ggattctgtt cttggaaaac tggagcattt gctcgcagag
      361 aatcttccag ttacaactcg ctggcagtac ttgctgcagc ctttcatgaa tgaagtgatc
      421 aatcatatca atcacaatct tcgcgatggc gcattcaatc ttcgcatgta ttatccggtg
      481 cgaaccatgg aagataaatt caagccttac tattcaaatg caatgcgtta cgcggtggag
      541 ctcatcgacg cggaaaagat tacttctgca tttgtgaaga gactttagtt gctgaattac
      601 atcagaaaaa actggagtag tattcttctt ttcgcttttg tcattggctt tgtgttgtat
      661 cgcaagatcc ctctctggga aaacgaaaga ggtcttgaag ggcagagtct tcctcacggc
      721 agtcttcgca cacttgctgg atctgagatt gacctatctt cgcgtaaaga gaaagtgatt
      781 cttgtttctt tctgggcaac ctggtgtatg ccgtgtcgcg tggaaatgcc aatgctcgtt
      841 tcgcttcgcc gcgaactcga tggacaatcc tttgagatct tcgcggttac agacgaagat
      901 ccaatcaccg ttgctcattt catagaggag catccagtca actttccgat cgttctggat
      961 cggggcccgc tgcagcaagc tttccggatt caggtctttc ccactctagt gcaattcaag
     1021 gatgggaagc ttgtttctgt ctcaaacgga gtagactggt ttttgaaatt taagattcgc
     1081 cacgcagtga ccggaaacta tttctgaccg caatgtcgga caacaggaag atcggtgtag
     1141 tcgattttgg cgggcaatat gcgcatttga ttgcttcgcg cattcgaagg ctcggagctc
     1201 tggctcagat tgcagatccg gaaactctaa ctccttctga ggcgcgcgct cagtacagcg
     1261 gtctcattta ttctggcgga ccttcttccg tgtacgagcc aggagctccc gcttctgatc
     1321 ctgctctttt gtccgcgggg cttccagttc tcggcatctg ttacggtcac cagctgatga
     1381 tgcagcagct cggcggtgag gttcgaaact caggccatca cgaatttgga cgcgccattc
     1441 ttaaagtgaa aagcgatgtg tctgttcttc gcggtgaaaa caactctgtt tccgctgtct
     1501 ggatgagtca tggagacgaa gtcacacgca tggcacctgg atttgttggg atcgcacata
     1561 ccgacgactg cgagaatgcg gcagtattca atgcggagaa gaatcttaca ggcattcaat
     1621 ttcatcccga agtgcagcat acagagcatg gagatcagta tcttctgaac tttattcgca
     1681 tgtgtggtct cgagaatagc tatcgcattg gagattttct ttcaaaggaa aaggaagctc
     1741 tggcgcgaag cattggagat cgcagtgttt tctttttact ttcgggtggc gtggattcaa
     1801 cggtagcgtt tactcttcta tctgagattc ttcctgcgga tcgactctac ggtcttttta
     1861 ttgataccgg attcatgcga aaggatgagg ggatagaagt gggtactgct cttcgtgcgc
     1921 atgggattca attgaacata gaggacgctc gcaaacgtta tgcgaccagt ctcgccggtg
     1981 tggtggaccc ggaaaagaaa agaagaatca ttggagatct attcctggaa atccaggcag
     2041 actgcgtgcg aagattgaat ctggacccgg aacactggct gctcgggcag ggcaccatct
     2101 acccggatac cattgaatct ggcggcacga agaaaagcca tttgatcaaa actcatcaca
     2161 accgggttcc ggcaattcag agatt
//
LOCUS       DAORFF010000619         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_39442_length_2165_cov_3.036019, whole genome shotgun sequence.
ACCESSION   DAORFF010000619 DAORFF010000000
VERSION     DAORFF010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39442_length_2165_cov_3.036019"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PKK76_18310"
     CDS             <1..664
                     /locus_tag="PKK76_18310"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNL70487.1"
                     /translation="TKSLESLIEERYRCRGFLPDGIDRTEIEKMIELAERAPSWKNAQ
                     SYKIVALTGEKKEALCRDLIERARSGAEPAAEIPYETGYPSSIRRRMMDLAVAFFAQL
                     GIDRKDKEAREKQTLRNFNGFGAPVMLFAFIPAGLREWTILDLGVFFGHLTLVVRSHG
                     YESILQAALAAWPDIVRKHTGVGAEYTLVVGMSIGKADPSEPANHFKSQRAPASEILQ
                     IL"
     gene            complement(708..1664)
                     /locus_tag="PKK76_18315"
     CDS             complement(708..1664)
                     /locus_tag="PKK76_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002737032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNL70488.1"
                     /translation="MATNYVRFRFRSSVDWGLLGEEGRIAPLRAGDLSTRAFLDFLRR
                     KKRPAPRFTTTVREVAILSPISAPAQIICQGANYRQHMIESGLNPDQKRYNMFFTKSD
                     ASLSPPIGNVIRPSHVRLLDYELELGLVFGRSIDHPIEVNAQNLSRYVAAIFMANDVS
                     ARDIQLPQMQWYKGKSYRTFLPAGPVLAVLSQADFAKLDLLELLLSVNGEIRQKDRAS
                     NLVFKPAETITELSQFCNVSPGDVLLTGTPSGCALRVPGQWQQRIASLLSEPKKWELF
                     LRGQLKRREYLKPGDQVESSIRTTDGSIDLGVQRWKVVQDQL"
     gene            1778..>2165
                     /locus_tag="PKK76_18320"
     CDS             1778..>2165
                     /locus_tag="PKK76_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70489.1"
                     /translation="MRKRLLLPLATCGFLTCVALPVKVAEVIPSAKNITIYSAGGSVV
                     SGTAAASSSDILKIRLPATAQGIAIVQNDQPLKWFTTQMISEPGKDPNVVQKYLQVGI
                     PAARTGEVKFSYGLPEIKWAPRLTASI"
BASE COUNT          558 a          562 c          568 g          477 t
ORIGIN      
        1 cactaaaagc ctggaatccc tcattgagga aagatacaga tgccgcggct ttctgcccga
       61 tggcatagac agaacagaaa tcgaaaagat gatagagctg gcagagcgag cgcctagctg
      121 gaagaatgcg cagagctata agatcgttgc tctgactgga gagaagaagg aggcactgtg
      181 tcgtgatcta atagaacgcg ccagaagcgg agccgagcct gccgctgaaa tcccctatga
      241 gacaggctat ccctcaagca ttcgccgccg gatgatggat cttgctgtgg cgttcttcgc
      301 gcaacttgga atagacagaa aggacaaaga agcacgcgag aagcaaactc ttcgcaattt
      361 caatggcttt ggcgctccgg tcatgctgtt tgccttcatt cctgccggtc ttagagagtg
      421 gacgattctt gatctgggag tgttctttgg tcacctaaca ctggttgtgc gctcacacgg
      481 atacgaaagc atcctgcagg ctgcactcgc tgcatggccg gacatagtgc gaaagcatac
      541 aggagtggga gcggagtata ctctggtagt tggcatgtct attgggaagg cagatccgtc
      601 cgagcctgca aatcacttca agtctcagag agcacctgcg tctgaaatac ttcagattct
      661 ttagtattga ggtttactgc tgaggagaac cgccgtcaca ggcggtctca cagctggtcc
      721 tggacgacct tccatctctg cacacccaga tcgatcgatc catctgtagt gcgaatgctc
      781 gactctacct gatcccctgg ctttagatac tcgcgtctct tcaattgccc acgcaggaac
      841 aattcccact ttttcggttc agagagtaaa ctcgctattc tctgctgcca ttggccgggc
      901 acgcgaagag cgcaaccaga tggagtacct gttaaaagca cgtcccccgg agatacgttg
      961 cagaactgag acagctcagt gatcgtctct gctggcttga aaaccagatt ggatgcccgg
     1021 tctttctggc gaatctctcc gttgacggaa agaaggagct ccagcaaatc cagcttcgca
     1081 aagtcagctt gcgaaagcac cgcaaggacg ggaccggccg gcaaaaaagt tctgtacgat
     1141 tttcccttgt accactgcat ctggggcaat tgaatgtctc tggcggatac gtcattcgcc
     1201 ataaagatcg ccgctacata tctagaaaga ttctgagcat tcacctcgat cgggtgatct
     1261 atcgatctac cgaacacaag cccgagctca agctcgtaat cgagcaaacg aacatgggaa
     1321 gggcgaatca cgttgccgat cggtggagaa agagacgcat ccgacttggt gaaaaacatg
     1381 ttgtatcttt tttgatcggg attgagtccg gactcgatca tatgctgcct gtagttggct
     1441 ccctggcaga tgatttgagc aggagcagaa atgggggaaa gaatagctac ttccctcacg
     1501 gtagtcgtaa accgtggcgc gggtctcttc ttgcgtctga gaaaatcaag aaaggcgcga
     1561 gtgctcaggt cacctgcgcg aaggggcgcg atcctgccct cctcacccag aagcccccag
     1621 tccaccgaag agcgaaagcg aaagcgcaca tagttcgtag ccatccatga gatggccgaa
     1681 cctggactac agcagtcaat tcaatatatc acaatgcgaa cgatcaaaac agtcagaatt
     1741 tgttgactcg gatctcgcgt gatcgcgaaa tcacgttatg cgcaagcgcc ttctattgcc
     1801 tctagccacc tgcggattcc tcacgtgcgt ggccctcccc gtaaaagtgg cggaagtgat
     1861 tccatccgca aaaaacatca ccatctatag cgcgggggga agcgtggttt cgggaacggc
     1921 cgcggcatcg agttcggaca tcttgaagat tcgtttaccg gccacagcgc aaggaatcgc
     1981 gattgttcaa aatgatcagc cgctcaaatg gtttacgact cagatgattt ccgaacctgg
     2041 caaagatccg aacgtagttc aaaaatattt gcaggttgga attccagccg cccgaacagg
     2101 ggaagtgaaa ttcagctatg gactgcccga aatcaaatgg gcccccaggc taaccgcctc
     2161 tatcc
//
LOCUS       DAORFF010000620         2158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_39704_length_2158_cov_3.189729, whole genome shotgun sequence.
ACCESSION   DAORFF010000620 DAORFF010000000
VERSION     DAORFF010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39704_length_2158_cov_3.189729"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PKK76_18325"
     CDS             <1..604
                     /locus_tag="PKK76_18325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNL70490.1"
                     /translation="IHDWPDLVAPGVTVILPNPKTGSSARWAYLAAYGQAKKTGGKAA
                     ADEYIRKLIAAVPIMDTGARAALTTFAERGMGDVFLSWENDAYLALHEHPDLQIIVPP
                     VSIRAEPAVAVVEKNAGPRLKAAQEYLTYLYSEEAQDLAGRHHYRPRSAAAASRYAST
                     FPNLILLDLTEVAGSWAQADKEHFADGASFDQAYQKRSRP"
     gene            696..1469
                     /locus_tag="PKK76_18330"
     CDS             696..1469
                     /locus_tag="PKK76_18330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70491.1"
                     /translation="MPILANKTPDELEPIILNALDKGQILSVQTYYLSDYGEKVLNLV
                     TSAILKKYGREDLIDVVYTSAKELVINATKSNLKRVLFARIGLDLFNPENYEQGMKHF
                     KEELKEENIRKYKSLFREFKFTVTITFYYSPDVLNIKIKNNTQLIPQEEERIRQKFAQ
                     AVSYANLFDFYLEHGDDTEGAGMGLTLVGILLNQSGIDKHQFTLYSSPHYNETTARLE
                     IPLSQEYTSRRERFEKERESSGLSREEFRKHFDSKKEFH"
     gene            complement(1463..>2158)
                     /locus_tag="PKK76_18335"
     CDS             complement(1463..>2158)
                     /locus_tag="PKK76_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase"
                     /protein_id="HNL70492.1"
                     /translation="IPGVGFDVVPTDCLARYVADASPGSENLEIAFAAISNPSQGTAK
                     SAIEMMPRGFLTRRNGDLQRISPGSGGKKVQFSDKERFVLPVSWGDLETAFYSTGIAN
                     ITTYTSLSRGASMFLRVFGPVMTLLFQFSPVRRSAKKFVDLFVRGPGAREQKSGRSYV
                     YVKVSSAAGKSREAWLETIEGYVFTAKSALLCVKEVIEKSPKGFKTPSVAFGYDLVLR
                     IPETRILDSIGNQ"
BASE COUNT          599 a          565 c          499 g          495 t
ORIGIN      
        1 cattcatgac tggccagacc tggtcgcccc tggtgtaacc gttattctcc ccaatccaaa
       61 aacaggaagt agcgcgcgat gggcttatct cgcggcctac gggcaggcaa agaaaacggg
      121 agggaaggct gcggcagatg agtacatccg caaactgatc gcagctgttc ccatcatgga
      181 cacaggcgca agagccgctc tcaccacatt tgcggagaga ggtatgggag acgtgttcct
      241 gtcgtgggaa aatgacgcat atctggcgtt acacgaacat ccagaccttc aaatcatcgt
      301 tccacctgta agcattcgcg cggaaccagc tgtagccgtt gtggagaaga acgcagggcc
      361 caggctgaag gcagcgcagg agtatctgac gtatctatat tccgaagagg ctcaggatct
      421 ggctggcagg catcactatc gcccgcggtc agctgcagct gcatctcgtt atgcctccac
      481 gttcccgaac ctgattctac tggacctaac cgaagttgcc ggcagctggg cgcaggcgga
      541 caaagagcat tttgcggatg gggccagctt tgatcaggcc taccagaagc gatcacgccc
      601 gtgaaatcgt agttgttttt gctacatgcc cggtattgac tggacgacaa taggagaggc
      661 tgtcaggttc tgcccgaggc actcatttca ctaacatgcc catcctggcc aataaaacac
      721 ccgatgagct tgagcccatc attctcaatg ctctggacaa aggccagatc ctttccgttc
      781 agacctatta cctcagcgat tacggcgaaa aagtactcaa tctggtcacc tccgctattc
      841 tgaagaagta cggccgcgaa gacctcatcg acgttgtgta tacgtccgca aaagaactcg
      901 tcatcaacgc gaccaagtcc aatttgaaga gagttctttt tgcccgcata ggcctggacc
      961 tattcaatcc agaaaactat gaacagggga tgaaacattt taaagaagaa ctcaaagaag
     1021 agaacattcg aaaatacaaa tcccttttcc gcgaattcaa gttcacagtg accatcacgt
     1081 tttattactc gcctgacgtt ctaaacatca aaatcaagaa caacactcaa ttgataccac
     1141 aggaagaaga acgcatccgc caaaagtttg cacaggcggt ttcttacgcg aatctctttg
     1201 atttctatct ggaacatgga gatgatacag aaggcgcagg catgggtctg actctggtag
     1261 gcattctact taatcaaagt ggaatcgata aacatcaatt cactctgtac tcaagcccac
     1321 actacaacga aaccactgct agacttgaga ttcctttgtc ccaggagtac acaagcagac
     1381 gcgaaagatt tgagaaagaa agagaatcaa gcggactgag ccgcgaagag tttcgtaagc
     1441 acttcgatag caaaaaagaa tttcactgat tcccaatcga atccagtatt ctggtttctg
     1501 gaatgcgaag cacgagatca taaccgaaag cgacggacgg tgtcttgaat cctttcgggc
     1561 tcttttcaat cacttctttc acgcaaagga gtgcgctctt cgctgtaaac acatagcctt
     1621 ctatagtttc caaccacgcc tccctgcttt tacccgcagc agaagaaact ttgacatata
     1681 catacgatct tccacttttc tgttctcttg cgccgggtcc tcgcacgaat agatccacaa
     1741 attttttggc cgatcgcctc acaggcgaaa actggaacaa caacgtcatc accggaccaa
     1801 acactctgag aaacatcgag gcaccgcgcg aaagcgaagt gtaagttgta atgtttgcaa
     1861 tgcctgtaga gtaaaaagca gtttccagat cgccccagga aacggggagc acgaatcttt
     1921 ctttgtctga aaattgaact ttctttccgc cgcttcccgg agagattctc tgcagatcgc
     1981 catttcttct ggtcaaaaag ccgcgaggca tcatctctat ggcagactta gccgtgccct
     2041 gacttggatt ggaaatagca gcaaaagcga tctcgaggtt ttctgatcca ggacttgcgt
     2101 ccgccacata tctggcgagg caatctgtgg gcactacgtc aaatcccact ccaggaat
//
LOCUS       DAORFF010000621         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_39734_length_2157_cov_3.758325, whole genome shotgun sequence.
ACCESSION   DAORFF010000621 DAORFF010000000
VERSION     DAORFF010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39734_length_2157_cov_3.758325"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PKK76_18340"
     CDS             complement(<1..952)
                     /locus_tag="PKK76_18340"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNL70493.1"
                     /translation="MEFLRRHCPYVLFSIPLIVSEVTALICNTAFGALIGSYLLGQPG
                     IYDASAASIVLGLLEVSSFVLSFVFLYPVFIASSRRKRGEEVPASLLKQARTRIYNFP
                     PFLIMLIWGVSFLEFLVTILSPELRKFGHFIPFVSYVLVAVSINAIVIYYLADSLNRF
                     VFFPDWFPDGNFDVTFRLRSPSLGFRFFDLFLVTGIFPVVTILGTFAVSRMQLAWDPD
                     AGRRALIVGVVMGGLYWLLGFVLLVLNSQSFLKPLNSMARAVKDLENAKYETRVQVHS
                     DDQIGNLERAINDMSRAIREKEMIKRVFGHYVSPAVRDMIL"
     gene            1066..1818
                     /locus_tag="PKK76_18345"
     CDS             1066..1818
                     /locus_tag="PKK76_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNL70494.1"
                     /translation="MSPIILLPVIVLGLMFLTQAIKILREYERGVVFRLGRFSAVKGP
                     GIIILIPIIDKMVRVSIRTIAFDVPPQDVITRDNVSIKVSAVVYFRVMDPDRAIIQVE
                     DYLYATSQLAQTTLRSILGESELDEILSRREKINAELQLVLDKQTDAWGIKVGIVEVK
                     QIDLPQEMQRAMAKQAEAERLRRAKIISAEGEFQASAKLSEAAAVIEKHPTAIQLRFL
                     QSLVEIAADKNSTIVFPVPIDLIHSILEKMKK"
     gene            complement(1819..>2157)
                     /locus_tag="PKK76_18350"
     CDS             complement(1819..>2157)
                     /locus_tag="PKK76_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70495.1"
                     /translation="AHSSPCIRLMHFEKHVFVCENKRDPSAPRPSCGHKNSTQLRNFM
                     KDKVKEMCPGRSIRVNMAGCLDRCEEGPSVVVYPDGKWFKIETEEQVVEFIRETLIEG
                     KTSTHLSMKE"
BASE COUNT          642 a          545 c          499 g          471 t
ORIGIN      
        1 cgaggatcat atctcgaacg gcgggagaca cgtaatgccc gaagacgcgc ttgatcattt
       61 ccttttcgcg tatggcacgg ctcatgtcgt tgatggctcg ttccagattt ccaatctgat
      121 catcgctgtg cacctgaacg cgagtctcat actttgcgtt ttccaggtct tttaccgccc
      181 gggccatgct gttgagaggc tttagaaaac tttgcgagtt tagaaccaga agcacaaatc
      241 caagaagcca gtacaggcca cccatcacaa cgcccacaat caaggcccgt cttcccgcgt
      301 caggatccca ggcaagctgc attctagaaa cggcaaacgt tcccagaatc gtaacgactg
      361 gaaaaattcc agtgaccaga aacagatcaa agaatcgaaa tcccagagaa ggagaacgaa
      421 gccggaaagt tacgtcgaaa tttccatctg gaaaccaatc gggaaagaaa acaaagcgat
      481 tgagactgtc tgccaggtag taaatcacga ttgcattgat gctcacagca accagcacgt
      541 aggaaacaaa tggaatgaaa tggccaaact tccgcaattc aggagaaaga attgtaacca
      601 gaaactcgag aaaggaaacg ccccagatca gcataatcag gaaaggagga aaattgtaga
      661 ttctagtgcg cgcctgtttc aagagactcg ctggaacctc ttctccgcgc ttacgcctcg
      721 aagatgcaat aaacacggga tataaaaaga caaaactcag aacaaaggaa gagacctcaa
      781 gaagtccgag tacaatggaa gcggcgctcg catcataaat tccgggctga ccgagcaaat
      841 aactgccaat gagtgcaccg aacgcagtat tgcagatcaa agcagtaacc tcggaaacaa
      901 tcaaaggaat gctaaacagt acatacggac aatgccggcg aagaaattcc atctgcttgc
      961 aagacaagca cgtggagagc gcatgctgtc aaccacttgc ccgcagatga atctcttgac
     1021 gccatgaaat caagaatcat cctgaggtca caaagagagg ttctcatgtc accgatcatc
     1081 ctactaccgg tcatagtact tgggcttatg tttctgacgc aggccatcaa aatacttcgc
     1141 gaatacgaac gaggtgttgt attccgtctc ggaagatttt ctgcagtcaa aggcccgggc
     1201 atcatcatcc tgattccaat catagacaaa atggtgagag tgagcattcg caccatcgct
     1261 tttgatgttc ctccacaaga tgtaatcacg cgagacaacg tttctatcaa agtgagtgct
     1321 gtagtttact tccgcgtcat ggacccggac cgggccatca ttcaagtgga agactatctc
     1381 tatgctacgt ctcagctcgc gcaaacaaca ctacgaagca ttctgggaga atcagaactg
     1441 gatgaaattc tatccagaag agaaaagatc aacgcagaac ttcagcttgt cctggacaaa
     1501 caaacggacg catggggaat caaagttgga attgtagaag tcaagcagat cgacctgcct
     1561 caggaaatgc agcgcgccat ggccaagcag gcagaggcag agagacttcg ccgcgcgaag
     1621 atcatttccg cagaaggaga attccaggca tccgccaaac tctcagaagc cgcagcagtg
     1681 atcgaaaagc atccaaccgc catccagctt cgctttctgc agagtctcgt tgagattgcg
     1741 gcggacaaga attcaacgat tgtgttcccg gtccccatcg atttgattca ctcgatactt
     1801 gaaaaaatga aaaagtaatt actctttcat ggaaaggtgt gtggaagttt ttccctctat
     1861 cagagtttcg cggatgaact ccaccacctg ctcttccgtt tctatcttga accattttcc
     1921 gtccggataa acaacaacgg acggaccttc ctcgcaacgg tccaggcatc ctgccatatt
     1981 gactcgaatg ctgcgacctg ggcacatttc cttcaccttg tctttcatga agttgcgcag
     2041 ctgtgtgctg ttcttatgac cgcaggatgg tctgggcgcg gacgggtccc ttttattctc
     2101 acaaacgaat acgtgctttt cgaaatgcat caaccgtata cacggcgaag aatgcgc
//
LOCUS       DAORFF010000622         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_40085_length_2148_cov_3.190635, whole genome shotgun sequence.
ACCESSION   DAORFF010000622 DAORFF010000000
VERSION     DAORFF010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40085_length_2148_cov_3.190635"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PKK76_18355"
     CDS             complement(<1..1081)
                     /locus_tag="PKK76_18355"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNL70496.1"
                     /translation="MEFLKKIRIAGFKSFADETIIDTGPGITAIVGPNGCGKSNILDA
                     VRWVLGERSPKGLRAKSMEDVIFLGSETRNAGGYAEVEITFDNTTRLLQLDQDEVSVG
                     RRVYVSQGSEYLLNGRRVPRREIERVLMDTGIGKTAYSIMEQGRMSEILKASPEDRRQ
                     LFDEAAGVSRFKAEREETIERLSATEQNLLRLDDILKGKREEMEHLDKQAKKTRQYLK
                     LKELLDKSDKKLRYIQYRDLIERKTAGETKLTELQRKRDELSTQIANREKMSEELSSL
                     QESESEEMHRLDRDYHQTLARIESLNSDRERLSAEVIERETKLHVLQGRKAAEEKNKL
                     AIEKKYETSTQLELDLGAEIESLRDA"
     gene            1157..>2148
                     /locus_tag="PKK76_18360"
     CDS             1157..>2148
                     /locus_tag="PKK76_18360"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNL70497.1"
                     /translation="MERNPEERIQSILKRKPYLQIFYGAKSGGNFQVLPDGSVTQANG
                     KAITSRMPALEAERLLPLDRQKWTGKEIILIQGLGNPVLPGLLANSLRENQICIALDH
                     RFDLASALVRGPLLSFLERPGCHVFAGEEMLELFRTYLESIPAENFRGVRRALHGSSM
                     REDAAFYSHVEENARSVLRSKMSDLLTRFEFEKTWLKHIVTNSRHLPPDGSGGPASIL
                     SYKDCLKGTPGMLVGAGPSLRRSRELLHAAHGKSYILACDTAAKVLLRFGIEPDGVAT
                     LDAQKHTIFHFLGADLKKVTLFADLVCNPLVFRHAEIGDVVFSTTAKYITGHDGT"
BASE COUNT          501 a          561 c          512 g          574 t
ORIGIN      
        1 aagcatcgcg aaggctttca atttccgcac ccaggtcaag ttctagctgg gtagatgttt
       61 catatttctt ttctatggcg agtttgtttt tctcttctgc ggcttttctt ccctgtaaaa
      121 catgaagctt ggtttctctt tcaatcactt ctgcagaaag tctttcccgg tctgagttca
      181 aagattcaat gcgagcgaga gtctggtggt agtcccggtc gagtctgtgc atttcttcag
      241 actcgctttc ctggagactg gaaagttctt cgctcatctt ctcgcgattt gcgatttgtg
      301 tggaaagttc gtctcttttg cgctgcagtt ctgtgagctt tgtctcaccg gcagtctttc
      361 tttcaatgag gtcccggtat tgaatgtatc tgagcttctt atcgctctta tccagaagtt
      421 ctttgagctt tagatactgg cgcgttttct ttgcctgctt atccaggtgc tccatttctt
      481 cgcgcttgcc tttgagtatg tcatcgagtc tgaggagatt ctgttctgtg gcagagagtc
      541 tctctatggt ctcttctctt tccgccttaa aacgagacac accggctgct tcatcaaaca
      601 attgtctgcg gtcttctgga gatgccttta gaatttcaga catcctgccc tgctccataa
      661 tggagtatgc agtcttgccg atccccgtat ccatcagcac tcgttcaatt tcgcgccttg
      721 gaacgcggcg accgttgagc aaatactctg aaccctggct tacgtatacg cggcggccaa
      781 cggaaacctc atcctgatcg agctgcaaaa ggcgggtggt gttatcaaac gtgatctcca
      841 cttccgcgta tccgcctgca tttctggttt cagagcccag gaagatcaca tcttccatgc
      901 tcttggcccg aagccctttg ggcgaacgtt cgcccaggac ccagcgcact gcgtcgagaa
      961 tattggattt accacaccca tttggcccga caatcgcagt gatgcctgga ccagtatcga
     1021 tgatcgtttc atccgcgaag gacttaaagc ccgcgatgcg aatcttcttc agaaattcca
     1081 ttggtaccgg atgcaaacgt gaaattcgtt cgcaaaaacc gacccaggca gcaaatcctg
     1141 cttcccggac cgtcgaatgg aaagaaatcc cgaggaacgc atccagtcca ttctaaaaag
     1201 aaagccctac ctccaaattt tttacggggc aaaatccggc gggaatttcc aggttctgcc
     1261 ggatggaagc gtcacccagg cgaacggaaa agcgatcact tcaagaatgc cggctctgga
     1321 agcagaaaga ctgctccctc tagatcgcca gaaatggaca ggaaaagaaa tcatactcat
     1381 ccagggtctc ggaaatccag ttctacccgg gctccttgca aattctcttc gagaaaacca
     1441 gatctgcatc gcacttgatc acaggttcga cctcgcgtct gcgctggtgc gcgggcccct
     1501 tctatcgttt ttggaaaggc caggatgtca cgtttttgcg ggcgaagaga tgctggaatt
     1561 gttcagaacg tatctggaat ccattccggc agaaaacttt cgcggagtga gacgagcgct
     1621 gcacggatcc tccatgagag aagacgcagc attctattct cacgtagaag aaaatgcgcg
     1681 aagtgttctc agatcaaaaa tgtcagacct gctcactcga tttgaatttg aaaaaacctg
     1741 gctcaagcat atagtcacaa actcgaggca tcttccacct gatggttcgg gtggtcccgc
     1801 aagcatcctt tcctacaaag attgtttgaa aggaactccg ggaatgctcg tcggggccgg
     1861 gccatctctt cgaagaagta gagaactgtt gcatgcagct cacggaaaaa gttatattct
     1921 cgcatgcgac actgccgcga aagttctact ccgatttgga atcgagccgg acggagtcgc
     1981 cacactggac gcgcagaagc acaccatctt tcatttcctg ggtgcggatc ttaaaaaagt
     2041 aacgttattc gccgatctgg tctgcaatcc tctggtcttc aggcacgcgg aaattggcga
     2101 tgtggttttt tccaccacag cgaagtacat cacaggccac gatggaac
//
LOCUS       DAORFF010000623         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_40214_length_2144_cov_3.611776, whole genome shotgun sequence.
ACCESSION   DAORFF010000623 DAORFF010000000
VERSION     DAORFF010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40214_length_2144_cov_3.611776"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKK76_18365"
     CDS             <1..283
                     /locus_tag="PKK76_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70498.1"
                     /translation="GFPDFPGTDSTRIFYAVLPDRDPDACLAAIQSAGFHKITALTGE
                     AFRPLPEALPAGATDKLLPLLDPWNIFVGSHRLYDSFLDLTRASTKDEH"
     gene            285..1586
                     /locus_tag="PKK76_18370"
     CDS             285..1586
                     /locus_tag="PKK76_18370"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNL70499.1"
                     /translation="MEMYIARRKSFQRNMEPGDVAIFQSAPSLIRSNDVHHRFRQDSD
                     FYYLTGFGEEQSALVLTKENAILYLRKRDPERETWNGPMLGVDRAVEALAVDEARDIT
                     DFKKDIPDLLRKKDRLYYMYGRNPELDLLIMSTCDQLMRRGRTGEFGPGQILHAASLL
                     HEMRMIKSQEEIELLRECAQIASDAHIALMRSAKAGMHEYELEGIILDSFRRHNAVEG
                     YPSIVASGKNACVLHHIENNRRIESNDLILVDAGAEKHCLNSDVTRTFPVADRFNGPQ
                     KDAYEIVLAAQLRAIEKTIPGATLDEIHNAAVRDLTQGLIDLGVLSGTLDENVENKKY
                     IRYYMHRTGHWLGMDVHDVGSYVKDGVPRKLEDGMVCTVEPGLYFPDDEQTPPAFRGI
                     GIRIEDDVVVRGNNPLVLSEMIPKTISAIEALRGGIHAERV"
     gene            1570..>2144
                     /locus_tag="PKK76_18375"
     CDS             1570..>2144
                     /locus_tag="PKK76_18375"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNL70500.1"
                     /translation="MLSEFKLPFTIPGTTIDSISTFSILLMIAFLTASYLAPRELKRR
                     GLREEAADWSILLAVFGAIVGSKIGFVFEVWSRIWIVDESWSNTLYYILFFKNGMGEK
                     YPGQAMGLWEALFSRGGLVFYGGLALTFLLIYIYLRRSFGKENVWRYGDALMPSLAIG
                     YAIGRLGCMVSGDGCYGYGSSYDIPLLTMVYG"
BASE COUNT          560 a          533 c          544 g          507 t
ORIGIN      
        1 cggctttccc gattttccgg gaacagattc gacgcgcatc ttctacgcag ttttgccgga
       61 tcgcgatcca gatgcatgcc tggcggcgat tcaatccgca ggatttcata agatcactgc
      121 acttacagga gaggcgtttc gacctctgcc agaagcattg cctgcgggag cgaccgacaa
      181 attattgcct cttcttgatc cctggaacat attcgttggt tcccatcggc tgtatgattc
      241 ctttcttgat ctaacgaggg ccagcacaaa ggatgaacat tgatatggaa atgtatatcg
      301 ccaggcgcaa atcgttccag agaaatatgg aaccaggaga cgtggccatc ttccagagcg
      361 cgccttctct cattcgctcc aacgacgtgc accatcgctt caggcaggat tcagatttct
      421 actacctcac tggatttggc gaagagcaat ctgcactcgt actgacaaaa gaaaacgcaa
      481 ttctatatct acgcaaacgc gacccggaaa gagagacatg gaacggtccg atgctcggag
      541 tagatcgcgc tgtggaagct cttgcagtag atgaagcgcg cgacatcacc gatttcaaaa
      601 aagacattcc cgatcttctt cgcaaaaaag accgcctcta ctacatgtat ggacgaaatc
      661 ctgaactcga cttgctcatc atgagcacgt gcgatcaact catgcgaaga ggacgcacag
      721 gtgaattcgg gccgggtcag attctgcatg cagcgtctct ccttcatgaa atgaggatga
      781 tcaagagcca ggaagaaata gaactactga gagaatgcgc acagatcgca tcagacgctc
      841 acattgcact catgcgctcc gcaaaagctg gcatgcacga gtatgagctc gaaggaatca
      901 tactcgattc ctttcgcagg cacaatgctg tggagggtta tccgtccatc gttgcgtccg
      961 ggaagaatgc atgcgttctt caccatatag agaacaatcg tcgaattgaa tccaacgatc
     1021 tcatcctcgt ggacgcgggc gctgaaaaac attgcctcaa cagcgacgtt acacgcacat
     1081 ttccggttgc agatcgattc aacgggcctc aaaaggatgc atacgaaatt gttctggcag
     1141 cacagctgcg cgccattgag aaaacaattc caggagccac tctggacgaa atccacaacg
     1201 cggcagtgcg ggacctgaca cagggattga ttgatcttgg agtgctgtct gggacgctgg
     1261 acgaaaacgt ggaaaacaag aaatacatcc gctactacat gcatcgcacg ggacactggc
     1321 tgggcatgga tgtgcacgat gtgggttcct atgtgaaaga tggagttccg cgcaaactcg
     1381 aagacggcat ggtttgcact gtggaaccag gtctttactt ccctgacgac gaacaaacgc
     1441 cacctgcctt cagaggaatc ggaatcagaa tcgaagatga tgtagtcgtt cgtggaaaca
     1501 accctcttgt attgagcgaa atgatcccaa aaacaatttc cgcaatcgaa gcattgcgcg
     1561 gaggaattca tgctgagcga gtttaaactc ccctttacca tccccggaac cacaatcgat
     1621 tccatttcca catttagcat tctactcatg attgcgtttt tgactgcgtc gtatcttgcc
     1681 ccgcgagaac tcaagcggcg aggcctgcgc gaggaggcag cagactggag catcctgctt
     1741 gctgttttcg gggcaatcgt tggatccaag attggatttg ttttcgaagt gtggagtaga
     1801 atctggattg tagacgaatc ctggtccaat acattgtact acatactatt ctttaagaac
     1861 ggcatgggag aaaaatatcc gggacaggcc atgggtctat gggaagcgct cttcagccgc
     1921 ggcggcctgg tattctacgg aggtcttgct cttacatttc tgctgatcta tatctatctg
     1981 cgtcgcagct tcggcaaaga aaacgtttgg agatacggag atgcactgat gccatcgctt
     2041 gccattggtt atgcaattgg aagactcggt tgtatggtaa gcggggacgg atgttacgga
     2101 tacgggtctt cctatgacat tcccctgttg accatggtct acgg
//
LOCUS       DAORFF010000624         2137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_40491_length_2137_cov_2.783381, whole genome shotgun sequence.
ACCESSION   DAORFF010000624 DAORFF010000000
VERSION     DAORFF010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40491_length_2137_cov_2.783381"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKK76_18380"
     CDS             <1..282
                     /locus_tag="PKK76_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HNL70501.1"
                     /translation="SSPEPALPVKLQELFGLRHTPKIARGKVPLTLHLLSPARRPIQI
                     TRDLEGFWTRTYPEVRKELRGRYPRHPWPEDPWTAQPTRFTRKRAEKSD"
     gene            complement(274..912)
                     /locus_tag="PKK76_18385"
     CDS             complement(274..912)
                     /locus_tag="PKK76_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70502.1"
                     /translation="MLPVLDKTASWKSLHTVRGAEPIRLSFFSDRAGYRLRIESERNE
                     VGVLFRLCAVLFVHGFSVVEGRIQSPRDLGIEDEFYLQLTDPSAILDERKASVMMKDF
                     ESLLFRGVSVLEYLIANQKKIPARTRKWKGTVELNFDEKPLLIVSGSDQPGLLLSLSQ
                     AFFLMDIDILEAVIHTDRMGVVRNQFLINPSDSRFGNAEFRKRLVEEIATLV"
     gene            1020..1622
                     /locus_tag="PKK76_18390"
     CDS             1020..1622
                     /locus_tag="PKK76_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HNL70503.1"
                     /translation="MKGSQANRIFEQSIVDYHKTDNVDQSLANPYNGVEGLLYGKCWI
                     DTVQWHLEDIIRSPNLAAEHFVATKRRIDKSNQDRTDSVEKLDDYFLERFQGVKPQAN
                     ARLNSETPAWLLDRMSILQLKIYHMREQTLRADVEEAHREQCKRKLSVLLDQEADMAQ
                     CFDELVDDLEKGIKKMKVYRQMKMYNDPKLNPILYGGAKA"
     gene            1624..>2137
                     /locus_tag="PKK76_18395"
     CDS             1624..>2137
                     /locus_tag="PKK76_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptosyltransferase"
                     /protein_id="HNL70504.1"
                     /translation="MRYLVLRFSAMGDVALLAPVLAALAKEHSRIAITLVTRKAFAPF
                     FYNIPGVEVIGADVDKEYKGLLGLYKLYRELMALGPYDHGIDVHGSIRSRILRLYFRL
                     SGLKFSAIVKGRREKSLQVRRYNKVLEPLPHMVERYMHVFDRAGISADPGRGPWINPD
                     TRCRALAMDYL"
BASE COUNT          565 a          516 c          533 g          523 t
ORIGIN      
        1 tcgagtccag aacctgcatt gcctgtgaag cttcaagagt tgtttggctt acgacacact
       61 ccgaaaatcg ccagaggaaa agtgcctctc acattgcatc tactttcgcc agccagaagg
      121 ccaattcaaa ttaccagaga ccttgaggga ttctggactc gcacgtatcc ggaagtgcgc
      181 aaagaactgc gcggcagata tcccaggcac ccctggccag aggatccctg gaccgcacag
      241 cctactcgat tcaccagaaa gagagcagag aaatcagact aacgttgcaa tctcttctac
      301 gagtctcttt ctgaactcgg cgtttccaaa tcggctgtca gatggattga tcaggaactg
      361 atttctcacg actcccattc tgtccgtgtg aatgactgcc tcaagaatgt ctatgtccat
      421 caggaagaat gcctgagata gagacaacag cagtccaggc tggtcagagc ccgatacgat
      481 caacagtggt ttttcgtcga aattcaattc aacagtgcct ttccactttc tggttctggc
      541 cggaatcttc ttttgatttg cgatgagata ttcgagcact gaaacccccc ggaacagtaa
      601 actctcgaag tccttcatca tcacagacgc ctttctttcg tctagaatgg cagacggatc
      661 tgtaagctgg aggtagaact catcctcgat tcccaggtcc ctgggcgact ggatgcgtcc
      721 ttccaccaca gaaaagccgt gcacaaacag aaccgcgcac aggcgaaaca gtactcctac
      781 ttcgtttcgt tccgattcga tcctgagtcg atatccggct cgatcggaga agaaggagag
      841 tcttattggt tcagcgcccc ggactgtatg tagtgatttc caggatgctg ttttatctag
      901 cactggtagc atgttttttt cttcctgagc gtgaaatttg caagtttcat gcccgcccgg
      961 caaagcgagt tgtgcgaaaa agggtttgag cccaccaggc cccgatccag agtgcaccgg
     1021 tgaagggaag tcaggcaaat cgtatattcg aacaatccat tgtggactat cacaagacag
     1081 acaatgtgga tcaatcactt gcaaatccat acaatggcgt agaagggctg ttatacggca
     1141 aatgctggat tgatacagtt cagtggcatc tagaagacat cattcgtagt ccgaatcttg
     1201 cggcagaaca tttcgtggca accaagcgta gaattgacaa gagcaatcag gacagaacag
     1261 attcagttga gaaacttgat gattatttct tagaacgatt ccagggcgtg aagcctcagg
     1321 caaatgcacg cctcaattcg gagactcctg cctggctttt agacaggatg tccattttac
     1381 agctgaagat ctatcacatg cgtgagcaga ctttgcgtgc ggatgtagag gaagcacaca
     1441 gggagcagtg caaaagaaag ctttctgtgc tgctcgatca agaagcagat atggcgcaat
     1501 gctttgatga gctggtagat gaccttgaaa aaggtattaa aaagatgaaa gtatatcggc
     1561 agatgaagat gtacaacgat ccaaagctca atccgattct gtacggcgga gcgaaggcct
     1621 gatttgagat acctcgttct tcgcttctcc gccatgggcg atgtggcgct acttgctccg
     1681 gttcttgcgg ccctggcaaa agagcactcg cgcatcgcca tcacactcgt tacgcgcaag
     1741 gcatttgctc cttttttcta caatattcca ggcgtagaag tgatcggcgc agatgtggac
     1801 aaagaataca aaggactgct tggtctttac aagctgtata gagaactgat ggccctgggt
     1861 ccgtatgatc atggaattga tgtgcatggc agcattcgaa gcagaattct gcgtttgtac
     1921 ttccgtctgt ccggcttgaa attctccgca atcgtgaaag gaagaagaga gaagtctctg
     1981 caggtgcgtc gctacaataa agtgcttgag ccgcttcctc acatggttga gcgctacatg
     2041 cacgtctttg atagagcagg catttccgca gatccgggaa gagggccgtg gatcaatcct
     2101 gatacacgct gtcgtgcact tgcgatggac tatctca
//
LOCUS       DAORFF010000625         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_40609_length_2134_cov_4.021164, whole genome shotgun sequence.
ACCESSION   DAORFF010000625 DAORFF010000000
VERSION     DAORFF010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40609_length_2134_cov_4.021164"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="PKK76_18400"
     CDS             complement(<1..1308)
                     /locus_tag="PKK76_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70505.1"
                     /translation="MRRNLFYAFSFLFCAGMILSRIWVTEDAYISFRAIENLYQGYGF
                     VYNPGERVEVTSHPLWAAILIVLRSMGLSLPMTSAVLGLFFTGLAIYQLIKRSMLADA
                     FPFAAIALCSISGFRDFSTSGLEFSLAFFLMTMFYIEVQEHGLLERPMYLAALLNLLY
                     LDRPETALLYAYYSVFFVLDLWRSTPRLASKIIPAIQWGLPAILITGSYHAFRYLYFG
                     ALFANPYYAKAGLDTYYSHGLKYLAHSLIFAPAITFALLLAASFLVLHRLRSKQKPLV
                     VGRAVRDLGAFALLTFYMIRVGGDFMAFRFWLPALAILFITVDRMFISVPRFPLPLLS
                     SRPVWTDWGALLFFAALSFWPVPMTRGFIADERPAFFSIGAGSVFGSKDNAWAARGRA
                     FRKLQDCLSYEDFWIANSQAHAHCLRGVGLGYVGVEAGPLVRIF"
     gene            complement(1361..>2134)
                     /locus_tag="PKK76_18405"
     CDS             complement(1361..>2134)
                     /locus_tag="PKK76_18405"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNL70506.1"
                     /translation="FLIATARNAENVPSYFWIIVSGALSGVLGAILWVLLPAERIGSN
                     IIFYGMFPQILLGASTMLVVPISGSAKRMEWRTKMLSIRLFEAAGLAGLFWVSNALYF
                     FSVKAAGMPAAIAATVWILTRVHAATPGEFHGILAKMFWTACILLCIGLIGRAFPWEF
                     SVHAAHVYWIGGLVMFLLSVSVQVLLSHGGHAMIPLPSRRTLVALLILVLLTVVTRVS
                     APVVPAMYISHLSYAAFVFLIVWILWLLRFARFLGSAKS"
BASE COUNT          671 a          555 c          524 g          384 t
ORIGIN      
        1 aaagatacgt acaagaggtc ccgcctctac tcccacataa cccagaccta cgcccctcaa
       61 gcaatgcgcg tgagcctgac tgtttgcgat ccagaaatct tcgtaagaaa gacaatcctg
      121 cagtttgcga aaggcgcgac ctcttgcggc ccatgcgttg tcttttgagc caaacacgct
      181 gcctgctcca atgctaaaga aggccggcct ttcatctgcg ataaaacctc tggtcattgg
      241 caccggccaa aaactcaacg ctgcgaaaaa cagcagggct ccccagtcag tccagacagg
      301 acgcgacgac agtagaggca gtggaaatcg cggaacagaa atgaacatac gatccacagt
      361 gataaataaa atcgcaaggg caggcaacca gaaccggaac gccataaaat ctccgcccac
      421 gcgaatcata tagaaagtca agagtgcaaa agcccccagg tctctgaccg cacgcccaac
      481 caccaatggc ttttgtttgg atcgcagcct gtgcagcaca agaaatgaag cagcgagtaa
      541 caacgcgaag gtgatggccg gagcaaatat tagcgaatgt gccagatact ttagaccgtg
      601 ggaatagtac gtatccaggc cggctttggc gtagtatgga ttggcaaaaa gcgctccgaa
      661 atacaaatag cggaacgcat gataagatcc tgtgatcaga atcgcaggga gtccccactg
      721 aattgccgga atgattttag aggccaggcg aggcgtggat ctccacagat cgagaacaaa
      781 gaatacagaa tagtacgcat atagaagcgc agtctctggt ctatccagat aaagcagatt
      841 cagcagtgcg gccagataca tgggtctttc gagcagtcca tgctcctgaa cctcgatgta
      901 gaacatggtc atcagaaaaa acgcgagcga aaactccagg cccgacgtag agaaatctct
      961 gaatccggaa atggaacaaa gtgcaatcgc agcgaatgga aacgcgtccg ccagcattga
     1021 tcttttgatc aattgataga tggccagacc cgtaaaaaag agtccgagga ctgcagaagt
     1081 cataggcagc gagagtccca tgctgcgcaa gacaatgaga atcgcagccc acagaggatg
     1141 actggttact tctactctct ctcccggatt atatacaaat ccgtagccct gataaagatt
     1201 ctcgatggcc cggaaggaga tgtatgcatc ctctgttacc cagattctgc ttaaaatcat
     1261 tcctgcgcag aacagaaagc tgaatgcata gaagaggtta cgacgcacag cttgacttaa
     1321 gaggggacag gatcgtgcaa agtaaaaaat aaactccggg tcagcttttg gcactgccca
     1381 gaaatcgcgc gaatcgcagc aaccagagaa tccatacaat caagaaaaca aaagcggcat
     1441 aactcaagtg agagatgtac attgcgggaa cgactggcgc cgacacgcgg gtaacaacgg
     1501 tcaacaatac cagaatcaga agagcgacta acgttctgcg cgagggaaga ggaatcatgg
     1561 cgtgaccgcc gtgcgaaagc aagacttgaa cagatacgga aaggagaaac atgaccaggc
     1621 ccccaatcca gtaaacatga gccgcatgca ccgaaaattc ccacggaaag gctctgccaa
     1681 tcaagccaat gcataacaga atacacgccg tccaaaacat ctttgcgagg attccatgaa
     1741 actcacccgg agtggcggca tgcacccgag tcaggatcca caccgtggct gcaatcgctg
     1801 ccggcattcc tgcggctttt accgaaaaaa agtacaacgc gtttgaaacc caaaaaaggc
     1861 cagccagccc agctgcctca aacaatcgaa tgctcaacat ctttgtgcgc cattccattc
     1921 tcttcgcaga gccactgatg ggaacgacga gcattgtgga agccccgagt agaatctgag
     1981 ggaacatgcc gtaaaagatg atgttcgagc caatccgctc tgccggaagc aaaacccaga
     2041 ggatcgctcc cagaactccg gatagggccc ccgatacaat gatccagaaa taagaaggga
     2101 cattttccgc gttcctggct gtcgcaatca aaaa
//
LOCUS       DAORFF010000626         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_41187_length_2119_cov_3.615310, whole genome shotgun sequence.
ACCESSION   DAORFF010000626 DAORFF010000000
VERSION     DAORFF010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41187_length_2119_cov_3.615310"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2119)
                     /gene="dnaE"
                     /locus_tag="PKK76_18410"
     CDS             complement(<1..>2119)
                     /gene="dnaE"
                     /locus_tag="PKK76_18410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNL70507.1"
                     /translation="EERTAALGNIEIAKKNGIRLALTNDSHFLKREDHSAQDILLRIN
                     QKKTIEDPTSFSFTPDFHVKTPAEMFGLFPEHPDAFYNTLRIAEMVDLNFKFGNPLLP
                     KFDVPQNYTLDSYLHHIAYEGLKKRYGTITKEVNDRFEFEFSVIQKMDFAGYFLIVQD
                     FINHAKSRDIPVGPGRGSAAGSIISYALGITDLDPIRYGLLFERFLNPDRKEMPDIDV
                     DFCVERREEVIQYVRDKYGKDRVGQIITYGTMAAKACLKDVARVLNIPFAESNDISKM
                     FPEELGIGIDDALAKSKDLKAYSEKGDIQKRLFTAARRLEDNVRHTGVHAAGVVIAPA
                     PLEELVPLATVASKDETTEGSRVLVTQYDMTALSEVGLVKMDFLGLRNLTVLHNTVRE
                     VERRTGKKIDLLNLPMDDEKTFKLLQRADIKGVFQLETSPGMRDFCSRIAPRRFEDLI
                     DLIALYRPGPLQSGMAESYIKRRKGQEKVSYPHPDVKEVLEDTFGVIIYQEQVMLISR
                     KIGGFTPGESDALRKAMGKKIHEKMQEMKAKFISGAQKLGHAEKFASDLFDQMAEFAS
                     YGFNKSHSAAYALIVYQTAYMKANYPTDTMRSLLESKVGKTEELVPYLNACHEMGVKI
                     LGPDVNESDVSFSYVEEGVIRFGLAAIKNAGELASKSVIEQRKSGKYKGLIDFLERVD
                     LRLCNRRTVEALVLSGGFDCFGYT"
BASE COUNT          488 a          494 c          507 g          630 t
ORIGIN      
        1 agtgtatcca aagcaatcaa atcctccgga aagtacaagc gcttccactg tgcggcgatt
       61 gcacagtctc aagtccactc tctcgaggaa atcaatgaga cctttgtatt tgcccgattt
      121 tctttgctcg atgactgatt tggaagcgag ttctcccgcg ttcttgatgg cagcaagacc
      181 aaatcgaatg acgccttctt ctacatacga gaagctcacg tccgattcgt tcacgtcggg
      241 cccaaggatc ttcacaccca tttcatggca tgcattgaga tagggaacca gctcttctgt
      301 ttttccaacc ttgctttcca ggagagaacg catcgtatct gttggatagt tggctttcat
      361 gtaagccgtc tggtaaacaa tgagagcgta agctgcagaa tgagatttgt tgaaaccgta
      421 agatgcgaat tcagccatct gatcaaacag atccgatgcg aacttttctg catgacccag
      481 cttttgcgca cccgagatga acttcgcttt catctcctgc attttctcat ggatcttctt
      541 acccatggcc ttgcgaagtg cgtctgattc tccaggtgtg aatccgccga tctttctaga
      601 gatcaacatc acctgttcct gatagatgat cacaccaaag gtgtcttcga gtacttcttt
      661 tacatccgga tgcggataag aaactttttc ctggcctttt ctacgtttga tgtaggattc
      721 cgccattccg gattgaagag ggcccgggcg atagagtgcg atgaggtcaa tgagatcttc
      781 aaaccttcgc ggtgctatgc gactgcagaa gtctctcatt cccggagaag tttcgagctg
      841 gaagactcct ttgatgtctg cgcgctgcaa cagcttgaaa gtcttttcgt cgtccatggg
      901 aagattgagc agatcaatct tcttgcctgt gcggcgttct acttctctca ctgtgttgtg
      961 taatacagtg aggtttcgaa ggccaaggaa gtccatcttc acaagaccga cttcagaaag
     1021 tgcagtcatg tcgtattgag tcacgagcac gcggctgcct tccgtggttt cgtctttgga
     1081 agcgactgtt gccagaggga cgagttcttc gagaggtgcg ggtgcgataa ctacacctgc
     1141 tgcgtggact ccggtatgcc gcacattgtc ttcgaggcgg cgagctgcag tgaagagtct
     1201 tttttgtatg tctccttttt cggaatatgc ctttaaatcc tttgattttg caagcgcgtc
     1261 atcaatgccg attcccagtt cttctggaaa cattttggat atgtcattgg attcagcaaa
     1321 aggaatgttg agcacgcggg ctacgtcttt cagacatgcc ttggccgcca tggttccgta
     1381 ggtgatgatc tgtcccactc tgtctttgcc gtatttgtcg cgtacatatt gaatgacttc
     1441 ttctcttctt tctacacaga aatccacgtc aatgtccggc atttccttac gatccgggtt
     1501 caagaatcgt tcaaatagaa gtccataacg aattggatcc aggtccgtaa tgcccagagc
     1561 gtaagaaatg atagaacctg cagcagatcc tcttccagga ccgacaggaa tgtctctgct
     1621 cttcgcgtgg ttgatgaaat cttgaacaat caggaagtag cccgcgaagt ccatcttctg
     1681 aatcacgctg aattcaaatt caaagcgatc gttcacttct tttgtaatgg tgccgtatct
     1741 ttttttcaga ccttcgtagg cgatgtgatg aaggtagctg tccagagtgt agttctgggg
     1801 tacgtcaaat ttgggaagga gagggtttcc gaatttgaaa ttcagatcca ccatctctgc
     1861 gatgcgaaga gtattgtaga atgcgtctgg atgttccgga aacagaccaa acatctccgc
     1921 cggtgttttt acatgaaagt ccggagtgaa ggaaaagctg gttggatctt ctattgtttt
     1981 tttctgattg atgcgaagca gaatgtcctg agcagaatga tcttctcttt tgaggaagtg
     2041 gctgtcgttt gtaagtgcca ggcgtatccc attttttttt gcgatttcta tattgcccag
     2101 agccgccgtt ctttcttct
//
LOCUS       DAORFF010000627         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_41197_length_2119_cov_2.902132, whole genome shotgun sequence.
ACCESSION   DAORFF010000627 DAORFF010000000
VERSION     DAORFF010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41197_length_2119_cov_2.902132"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PKK76_18415"
     CDS             <1..438
                     /locus_tag="PKK76_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNL70508.1"
                     /translation="PDSILRNYFVLLTDYTVAQIEGWIADPFEAKKILAATIVDDYHP
                     AGSGKEARLLWEKEKSERKGLVLPPDAKVLDLKGKSEISLVQAMVDGGVEASLTAARK
                     LIEQGAVKMGDDLETITSRDYVLKLPGNYSFKIGKKKYLVVQG"
     gene            435..>2119
                     /gene="mnmC"
                     /locus_tag="PKK76_18420"
     CDS             435..>2119
                     /gene="mnmC"
                     /locus_tag="PKK76_18420"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HNL70509.1"
                     /translation="MKPLFSAPVVPAIIEWRDDEPWSTVYQDVYFSKKAGIEESTAVF
                     IRGNDLETRFKNPGNFSILETGFGTGLNFLIASDLFERTSPSGLLHFFSVEKHPLKKE
                     DLIRALRTYPGLPCETLIERYAPWTHGFHHIVLSPRVILTLMIGDVSEILPLVRGRFD
                     ALFLDGFAPRQNEDMWSREIALELARISGPGTTLASFTSAGHVRRALIEAGFAVEKTP
                     GFGTKREMIRGSMPGTPRRQTPSHAVVIGAGIAGASTAFSLAKRGVPCTIIDRQDKPA
                     REGSGNRRGVFQPVLAASPTPWSRWTLAGFEYLCSLLRSLNFPTNFCGVFQAAEDEKE
                     LQRIQRAISVWPAELGRILSRPEASRVTGVDIPFPGVLFPGAGFLSPSDLTQRLILQS
                     NAQTLYGQSVARVEKMDSGFRITTAQTTVDADCLFYCAGTGTPQFVDAPVSATRGQIH
                     YIPPWNETLRTVICADGYLISGNDFWTAGSTYSRTDTTSSPHIEGQNEILEKLRNAIP
                     DVPHFDTLLDGRVGYRISAKDRFAIFGKLGDHAFLSTAHGSRGIVSAPLAAEV"
BASE COUNT          538 a          547 c          543 g          491 t
ORIGIN      
        1 ccagattcta ttttgagaaa ctatttcgta ctacttacgg attatacggt tgcacaaatt
       61 gaaggctgga ttgcagatcc gttcgaggca aaaaaaatcc tggcggctac catcgtagat
      121 gactaccatc ctgccggcag cggcaaagaa gcgcgattgc tctgggaaaa agaaaaaagc
      181 gaacgaaagg gactggtttt gccgccggac gcaaaagtgc tcgacctgaa agggaaatca
      241 gaaatcagcc tggttcaggc aatggtggac ggcggagtgg aggccagtct tacggcagcg
      301 cgaaaactca ttgagcaggg cgccgtaaaa atgggagacg atctcgaaac cattacgtcc
      361 agagactatg tgctgaaact gccaggtaat tactctttca aaatcggaaa gaaaaaatac
      421 ctggttgttc aggggtgaaa ccgctcttca gcgccccggt cgttccggcg atcatagaat
      481 ggcgcgacga tgaaccctgg tctacagtct atcaggacgt atatttctct aaaaaagcag
      541 gaattgaaga aagcactgca gtattcatcc gcggaaatga tctagaaact cgtttcaaga
      601 atcctggcaa cttttccatt ctggagaccg ggtttggcac tggcctcaat tttctgatcg
      661 cttctgatct atttgagaga acgtctccct ccggccttct tcactttttt tccgtggaaa
      721 aacatcctct caaaaaagag gatctaatcc gagcacttcg cacctatccc gggcttccat
      781 gcgaaacgct catcgaacgt tatgcgccat ggactcatgg atttcatcac atcgttcttt
      841 cccctcgcgt gattctgact ctgatgattg gagatgtgtc ggaaatcctg ccgctggtcc
      901 gcggaagatt cgatgcgctc ttcctggatg gctttgcgcc cagacagaat gaagatatgt
      961 ggtcacgcga aattgctctg gagcttgcgc ggatttctgg tcctggaacc acactcgcct
     1021 cctttacgtc tgcgggacat gtacgccgcg cgctcataga agccgggttc gcagtagaga
     1081 agactccagg ctttggaaca aagagagaga tgatcagagg gtccatgcct ggaacgccac
     1141 gccggcagac cccttcgcat gcagttgtaa ttggagcggg gattgcgggc gcgagcactg
     1201 ctttttccct ggccaaaaga ggagtgccct gcacgatcat agacagacag gacaagcctg
     1261 cgcgggaagg ttctggaaac agaagaggag tcttccagcc ggttctggcc gcttcgccta
     1321 cgccatggag cagatggact ctggctggat ttgagtatct atgctctctt ctgcgctcat
     1381 tgaattttcc cacaaatttt tgcggagtct tccaggcggc ggaagatgaa aaagaattgc
     1441 agcgaatcca aagagctatc tctgtctggc ctgcggagct gggacgaatc ctttcaaggc
     1501 ccgaagcgtc cagagtgaca ggggttgaca ttccatttcc tggagttctg tttcccggag
     1561 ccggattcct ttccccttcc gacttaactc agagattgat cctacaatcc aatgcacaga
     1621 cgttatacgg ccaatcggta gctcgcgtag aaaaaatgga ttccggtttt cgcatcacaa
     1681 ccgcacaaac cacagtcgac gcagactgcc tcttctactg cgccggaacg ggcaccccgc
     1741 aattcgtaga cgcaccggta agcgccaccc gcggacaaat ccactacatt ccgccctgga
     1801 acgaaactct acgaacagtg atttgcgcgg acggatactt gatttctggc aacgatttct
     1861 ggactgccgg gtctacctac agcaggacag acaccacatc atcgcctcac atagaaggcc
     1921 agaatgaaat cctcgagaaa ttgcgaaacg caattccgga tgtgcctcat tttgacactc
     1981 tactggatgg aagagttgga tatcgcatat ctgcgaaaga cagatttgcc atcttcggaa
     2041 agttaggcga tcatgcgttt ctatccaccg ctcacggttc caggggaatt gtaagtgctc
     2101 cccttgcggc cgaggtgat
//
LOCUS       DAORFF010000628         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_41261_length_2118_cov_2.059137, whole genome shotgun sequence.
ACCESSION   DAORFF010000628 DAORFF010000000
VERSION     DAORFF010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41261_length_2118_cov_2.059137"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PKK76_18425"
     CDS             <1..307
                     /locus_tag="PKK76_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70510.1"
                     /translation="YEQKAWTREALDFDVMRTIVQMNRKEDLVWFVPEFDTVTVPSEV
                     EAMKKAGVTPRPLFQTDYFLLQTDSPRRTSMERIFFLKSPFTHPSSSALLAIADAVK"
     gene            complement(285..1241)
                     /locus_tag="PKK76_18430"
     CDS             complement(285..1241)
                     /locus_tag="PKK76_18430"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL70511.1"
                     /translation="MTNKKSIILLTLALASCFSAKPRKQMQLFYRPPGTTVEIYREEP
                     VSLGINVYPPPPGPIRGAVIFVHGGGWMMPGTDLPLFEDWEQPLKDAGLQAFSIEHRT
                     APEYRGRELVEDCAQAVRYVHQNAARFKIPEEKIALIGFSSGGHLSVMTALTLTKQDP
                     ALGVRQASYLKAVVSFYAPLDLEELAKTGGPEIRTILANYLPIKNPGEEGDESKQRAM
                     RMRSMADFSQDSFLKRTLYDISPIENIHPAMAPTFLIHGDSDSLIPMMQSVQFYRRAY
                     AAGASVAFLGVPGAEHNFQQSRKPYARQAEAAAIQFILQHLQ"
     gene            complement(1246..1557)
                     /locus_tag="PKK76_18435"
     CDS             complement(1246..1557)
                     /locus_tag="PKK76_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002074700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNL70512.1"
                     /translation="MDSNEITQKLWEEIATVEDPELHMSIRDLGLIYEVLAENGIAHV
                     KMTLTSMGCPAGPELTAGVQAACTRVEGIDSAEVEVVWTPKWDPKEMASEEARMMLGI
                     Y"
     gene            1589..>2118
                     /locus_tag="PKK76_18440"
     CDS             1589..>2118
                     /locus_tag="PKK76_18440"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL70513.1"
                     /translation="MRNISSAIILLAASALRADNLDLAVQEYRSSLPERARYHLAVAS
                     RALAPGQIATAQKLEGLLFLSRGDLPNAEISFRNSLNRKPDAFLFYVLGRMMLDLRRY
                     DEAAEYYEKALKNSTDQKADSKTDSSRVDLLPFHCAETSDSSSWSAMFGDLTKFAQIW
                     DASLSRMERMAAASQAG"
BASE COUNT          498 a          532 c          534 g          554 t
ORIGIN      
        1 atatgaacag aaggcatgga cgcgcgaagc attggatttt gacgttatgc ggacaatcgt
       61 gcagatgaat cgaaaagaag atttagtgtg gtttgtgcct gaatttgaca cggtcactgt
      121 tccgtcagaa gtggaagcaa tgaagaaagc aggagtgact cccaggccgc tctttcaaac
      181 ggattatttt ttactgcaaa cggacagtcc gcgtcgtacg tccatggaac gtatcttttt
      241 tctcaaaagc ccattcaccc acccgagttc ttccgcgctt cttgctattg cagatgctgt
      301 aaaataaact gaatcgcggc tgcctctgcc tgccttgcat agggctttct ggattgttga
      361 aaattatgct ccgcgcctgg tactccaagg aaggcaacgg acgcccctgc cgcataggca
      421 cgtctgtaaa attgtacgct ttgcatcatc gggatgaggc tgtccgaatc tccgtggatg
      481 agaaacgtgg gcgccattgc ggggtgaata ttttcaatgg gagaaatgtc gtataacgtt
      541 cgttttaaga aagagtcctg cgaaaagtcg gccatacttc gcatgcgcat ggccctctgt
      601 ttgctttcgt ctccttcttc tccaggattt ttaattggta gataattcgc aaggatggtg
      661 cgaatttccg gacctccggt ctttgcgagt tcttcgagat ccagaggagc gtagaaagac
      721 acaaccgcct tgagataact tgcctggcgt acacctagag cagggtcttg cttcgtaaga
      781 gtcaacgcag tcattacaga caaatggcct cccgacgaaa aaccaatcag cgcaatcttt
      841 tcttccggaa tcttgaatcg tgccgcattc tggtgaacgt atcgcactgc ctgcgcgcag
      901 tcttctacga gttcgcgtcc acgatattcc ggagccgtgc gatgctcgat ggaaaatgcc
      961 tggaggcccg catctttcag aggttgttcc caatcttcaa aaagaggcaa atcggtgccg
     1021 ggcatcatcc atccaccgcc atgcacgaaa atgacggctc cgcgaattgg accgggagga
     1081 ggcggatata cgttgatccc gagggataca ggctcttcgc gataaatttc taccgttgtc
     1141 ccgggagggc gatagaatag ttgcatttgc ttgcgaggtt tcgcggagaa acaactggcg
     1201 agagccagag tcaaaaggat tatggacttc ttattggtca tccgttcagt agatgccaag
     1261 catcatccgg gcttcttcag aggccatttc ctttggatcc cattttggcg tccagaccac
     1321 ttccacttcc gcagaatcaa tgccttctac tcgcgtgcag gctgcctgaa cgccagcggt
     1381 caattcgggg ccagcaggac accccatcga agtgagtgtc attttgacat gcgcaatccc
     1441 gttttcggcc agaacttcgt aaatcaagcc aagatctcgg atgctcatgt gaagttccgg
     1501 gtcttcgacc gttgcaattt cttcccagag cttttgcgtg atttcgtttg aatccattct
     1561 taattccacc gatgctggaa ttaagatcgt gcgcaatatc tcttcggcaa tcattttact
     1621 ggcagcgtca gcgcttcgtg cagacaatct ggacctcgct gtacaggagt atcgatcttc
     1681 tcttcccgaa cgtgccagat accacctggc cgttgccagc cgcgcacttg ctccgggtca
     1741 aatcgccact gctcagaagc tagaaggcct tctgtttttg tccagagggg accttcccaa
     1801 tgctgagatt tcttttcgga attcactgaa tcgaaagccg gatgcttttc tgttctatgt
     1861 tcttggtaga atgatgctcg atctgcgtag atatgacgag gctgcggaat actacgagaa
     1921 agctctgaag aactcgacgg atcaaaaagc agattcaaag acagattctt cccgggtgga
     1981 cttgcttcca tttcattgtg cggaaaccag tgattcttcg agctggtctg cgatgtttgg
     2041 cgatctaaca aaattcgctc aaatctggga cgcttctctt tcgcgcatgg aaagaatggc
     2101 cgccgcatcg caggcagg
//
LOCUS       DAORFF010000629         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_41370_length_2114_cov_4.485187, whole genome shotgun sequence.
ACCESSION   DAORFF010000629 DAORFF010000000
VERSION     DAORFF010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41370_length_2114_cov_4.485187"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="PKK76_18445"
     CDS             complement(<1..975)
                     /locus_tag="PKK76_18445"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNL70514.1"
                     /translation="MKQPMVKMMRHARAIISRRSARVVFGGLGLAGAVLAFGVFAQAP
                     GKGSDRGAEQLLRNMGGDVGQDLDPMIPAPGKDLTPDFGALERERQDAKGTVIRNRKQ
                     DQVATAGDKNRPERDRVRKDKGTTQKKKDDQKPVALAEERALPGFPTNLDPTPNPGFT
                     HDAPSFYRGIYLTNEVIRIPSMYEPWLKAAHDAGLNTLVIDVQLVGKDHKAIERLPPE
                     AFMKLAKSYGFYMVARVVCFQGGLETYPPSMALLDSVLRTAENAARMGFMEVQLDYIR
                     FADNRNLGYVTLNQRYRMIAGILRMATDRLRPYGIRVGADVFGRIVFNN"
     gene            complement(1050..1997)
                     /locus_tag="PKK76_18450"
     CDS             complement(1050..1997)
                     /locus_tag="PKK76_18450"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL70515.1"
                     /translation="MIDVNADRIRRKDLIKLVKEVSRLERKLAREQNNRTRTEATKDK
                     LQVLMKQIHLEIEESHAEIQKKNHELEILYADLEKEKKKSDDLLLNILPEEVADELKR
                     SGVVAPQHFDSVSVLFTDFKGFTQASEHLAPSQIVAELDACFTAFDEIIERHGLEKLK
                     TIGDSYMCAGGIPNEAPTHPIDTMLAALEIRDYMNRLKIQRIEEGLPYWELRIGIHVG
                     PLVAGVIGRRKFAYDIWGDTVNTASRMESSGLPGMVNVSAALEQAVSPYFQTSLRGKI
                     PAKNKGEIEMHFVHRLKPEYSSDPAGIHPNRKVVCYRLS"
BASE COUNT          491 a          551 c          532 g          540 t
ORIGIN      
        1 gttgttgaac acgattcttc cgaatacgtc tgcgcccacg cgaattccgt acgggcgaag
       61 cctgtcggtt gccatgcgaa gaatcccggc gatcatgcga tagcgctgat tgagagtcac
      121 atatccaaga ttgcgattgt ccgcgaaacg aatataatcc agctgcactt ccatgaagcc
      181 cattctggct gcattttccg cagtcctgag aacggaatcg agaagcgcca tagaaggagg
      241 atatgtctca agcccgccct ggaagcaaac gactctggcc accatgtaaa aaccataaga
      301 ttttgcaagt ttcatgaacg cttcgggcgg aagcctttcg atggccttgt gatctttccc
      361 aacgagttga acatcaatga cgagtgtgtt gaggcctgca tcatgggctg ctttcagcca
      421 gggttcgtac atggaaggaa ttcggatgac ttcattggtc agataaattc cgcgataaaa
      481 agaaggggca tcgtgcgtga aacctggatt cggagttgga tcgagattcg ttggaaaccc
      541 tggcaaagca cgctcttcgg cgagtgccac tggcttctgg tcatctttct tcttctgggt
      601 tgtccctttg tccttgcgta cgcggtcgcg ctctggccga tttttgtcac cggcagtggc
      661 tacctggtcc tgcttgcgat tgcggatgac tgtgcctttt gcatcctgac gttcgcgctc
      721 gagagcgcca aaatcagggg tcaggtcttt tcctggagct ggaatcatgg gatccagatc
      781 ctgtccaacg tccccaccca tattgcgcag aagctgctct gcgccccgat ccgatccttt
      841 gcccggggcc tgggcaaaaa ctccgaaagc cagtacagcg ccggcaaggc caaggccccc
      901 gaacaccact ctggcagatc tacgagaaat gatagcgcgt gcgtgacgca tcatcttaac
      961 catcggctgt ttcatataaa aagagtgaac cggagccagt tttttgtaag gagccaggcg
     1021 ggaaagccaa aacaggaccc tggatcgact caggaaaggc ggtaacagac taccttgcga
     1081 tttggatgaa ttcctgcagg atccgacgaa tactctggtt tcaaacgatg cacaaagtgc
     1141 atttcgattt cccctttgtt cttggcaggg atctttccgc gtagcgaagt ctgaaagtaa
     1201 ggcgacactg cctgctcgag ggccgccgat acgttgacca ttcctggcag gccagaggac
     1261 tccatgcgcg acgcagtgtt gactgtatcg ccccagatgt cgtaggcaaa cttgcgtctt
     1321 ccaatcacgc ctgcgacgag cggtcccacg tgtatcccaa tacgcagctc ccagtaagga
     1381 agtccttctt caattctctg gatcttcaaa cgattcatgt agtcacgaat ctcaagcgcg
     1441 gcgagcatcg tgtcgattgg atgtgtggga gcttcgtttg gaattccacc cgcacacatg
     1501 tagctgtctc caatggtttt gagtttttca agaccgtgtc tttcaatgat ttcatcaaat
     1561 gcggtgaagc atgcatccag ctctgctacg atttgagaag gagcaaggtg ttcggacgcc
     1621 tgagtgaatc ccttgaaatc agtaaacagc acactcacag agtcaaagtg ttgaggagca
     1681 acgactccgg aacgtttcaa ttcatccgcc acttcctccg gaaggatatt gagaagtagg
     1741 tcgtctgact tctttttctc tttttcaaga tccgcgtaaa gaatttcgag ctcatgattc
     1801 ttcttttgta tttcagcgtg tgattcttct atttccagat gaatctgctt catcaatacc
     1861 tgaagcttat ctttggttgc ctctgttctg gtgcgattgt tctgttctcg cgcaagcttc
     1921 ctctcaagac gcgaaacttc tttcaccaac ttgatgaggt ctttgcgtct gatcctgtct
     1981 gcgttcacat cgatcatgat ctaccccaga agaagagtaa caaatgtagc attgtggaag
     2041 caagtgggac cacctcgctc aagcggagcg atttccccat agacgtaaaa tccgccggcg
     2101 cagcatccgc ccgg
//
LOCUS       DAORFF010000630         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_41539_length_2110_cov_2.504623, whole genome shotgun sequence.
ACCESSION   DAORFF010000630 DAORFF010000000
VERSION     DAORFF010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41539_length_2110_cov_2.504623"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1518
                     /locus_tag="PKK76_18455"
     CDS             148..1518
                     /locus_tag="PKK76_18455"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL70516.1"
                     /translation="MNDIQKLILAGGFLLLTGGIALLTWERFGSGYPLRFMEVERNES
                     VIEKADRQLKEAEEYLRQNSRDSYAKAKAICTEVLSRNLNDRINQIARYELAEALEKL
                     DDNAAALEHYRALKQDQDKIQSQELRDKVDYSLGRLLLVINHEVEGRSLLESLLARTN
                     DGRLKSRIHTAFGQFYLARGDRQRAQENFNIALKYFPENLQAEIGRTGAVNGQRKSMY
                     YEYYDEYLTGNANLSPDKRAAQSDLRLSTYEAGIRAYKTGRYQDAVDAFGRVLKDGRD
                     NVEAEKSLYWTGESLQALGRTREAYETYERVLGNATSVMDQAALIRMGMILYQQGKLQ
                     EALTRFYRASENYPDGAYTSRALEWKRETEAQIREHDFLKNYDDRKKEDPKTDTRPQS
                     RLLEHADSEIAVVREEDRARNVASADTTSTASSENSDADKSEMIIEVDGKEIKLAERK
                     PQAVRR"
     gene            complement(1478..>2110)
                     /locus_tag="PKK76_18460"
     CDS             complement(1478..>2110)
                     /locus_tag="PKK76_18460"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloenzyme"
                     /protein_id="HNL70517.1"
                     /translation="RVIQDHSIIKVLKDKGKKEALANAFSEAYLERIARKPRLTGAST
                     HVHLASGQPLKNLDDLENRKALYMDIPHEVMHRFFPHLKERFPVRNAEEVGRDAGMLA
                     RDYDMLIYEFFLSDKAGHNQSFEEAEWAIRTLESFLKGLCEVLRPEDTLLITSDHGNL
                     EDLSTKTHTKNPVLALAYGRGSDGLIEKVRDISDFPSFIYELLGVSVPRA"
BASE COUNT          589 a          493 c          547 g          481 t
ORIGIN      
        1 gctttacacg gggatcagtt attacaaact cggacaatac aggactgcga ggcgctactt
       61 tattgatccg gtcgtgcgga aggaacttcc ggacagatct acattctgga acaagaggag
      121 cctcgagaga ctgccatgag taattcgatg aatgatattc aaaaactaat tcttgcaggt
      181 gggttcctgc tactgaccgg agggattgcc ctcttgacct gggagcgatt cggaagtgga
      241 tacccgcttc gctttatgga agtggaacgc aatgaaagcg taatcgagaa ggcggacaga
      301 cagctcaaag aagcggaaga ataccttcgc cagaattccc gcgattcgta cgcgaaagca
      361 aaagcgatct gcacggaagt actttcgcgc aatctcaacg atcgcatcaa tcagatcgcg
      421 cgctatgaac ttgcagaggc acttgagaag ttagacgaca acgcagcggc cctggaacat
      481 tacagagcgc tcaagcagga tcaggataag atccagagcc aggaactgcg cgacaaagtg
      541 gactattctc tgggacgcct tcttctcgtg atcaatcatg aggtagaggg caggtctctg
      601 cttgaatcgt tgcttgcacg caccaacgac ggacgtttga aatcaaggat tcatactgca
      661 tttggccagt tctatctggc aagaggggat cgtcagagag ctcaggaaaa tttcaacatt
      721 gctctgaagt atttcccgga gaacttgcaa gcggaaatcg gaagaaccgg cgcagtgaac
      781 ggtcagagaa agtctatgta ctacgagtac tatgatgaat acctgacagg caatgcgaac
      841 ctttctcccg acaaaagggc cgcacagtcg gatctcagat tgtctacata cgaagccgga
      901 attcgcgctt acaaaacggg taggtaccag gatgcagtgg atgcattcgg gcgcgttctt
      961 aaagacggac gtgataacgt ggaagcagag aaatctcttt actggaccgg ggaaagtctc
     1021 caggcactgg gccgcactcg cgaagcatac gaaacatacg aaagagttct tggaaatgca
     1081 acatccgtga tggatcaagc ggctctcatt cgcatgggaa tgatccttta tcagcagggt
     1141 aaactccagg aagctcttac tcgcttttat cgcgcatcag aaaactatcc agatggagca
     1201 tacacttctc gtgctcttga gtggaaaaga gaaacagagg cccagattcg ggaacacgac
     1261 ttcttgaaaa actatgatga tagaaagaaa gaagacccaa aaacagatac gcgtccgcag
     1321 agtcgtcttc ttgaacatgc tgattcagaa attgcagtag tgcgcgaaga agatcgcgca
     1381 cgaaatgtgg caagcgcaga cacaacttcg actgcatcca gcgaaaactc tgatgcggac
     1441 aaatctgaaa tgatcataga agtagacgga aaagaaatca agctcgcgga acggaaaccc
     1501 caagcagttc gtagataaaa gaagggaaat cagaaatatc cctgaccttc tcgatcaacc
     1561 cgtctgaacc ccgtccatag gcaagagcca gtacggggtt ttttgtatga gtcttcgtgc
     1621 ttagatcttc caggtttccg tgatcagaag tgatgagcag tgtgtcctct ggcctgagca
     1681 cttcgcacaa ccctttcaag aaagattcaa gagtgcgaat ggcccattct gcttcttcga
     1741 acgactggtt gtgtcctgcc ttgtctgata ggaaaaattc atagatgagc atgtcgtagt
     1801 cgcgggccag catccccgcg tctcgaccta cttcctccgc gtttcgcact ggaaatcttt
     1861 ctttgagatg aggaaagaat cgatgcatca cttcatgggg gatgtccata tagagtgctt
     1921 tgcgattttc gaggtcgtct agattcttca gaggttgtcc ggacgcgagg tgcacatggg
     1981 tggaggctcc cgtcagtctt ggctttctgg caattcgctc caggtaggct tcgctaaagg
     2041 cattcgccag agcttccttc ttgcctttgt ctttcagcac tttgatgatg gaatgatcct
     2101 gaatcacccg
//
LOCUS       DAORFF010000631         2105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_41726_length_2105_cov_4.068780, whole genome shotgun sequence.
ACCESSION   DAORFF010000631 DAORFF010000000
VERSION     DAORFF010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41726_length_2105_cov_4.068780"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PKK76_18465"
     CDS             <1..648
                     /locus_tag="PKK76_18465"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNL70518.1"
                     /translation="LAVLVANGTRSAQIGIAFALCVSAAEFLFRTNRRLFFAAAIAAT
                     MLISLVYLYGDFSGRQTDFQRPILWTGAGAIFENHPWIGTGPGGFASAILEWKKTFNL
                     SHPMSWYYVESTPSGHAHSDLLHLSAIGGIPGCLFFLSIVALSVRTVASQKSEAILSR
                     SALIAFFAAGVAQCYFQDDEVVVIYWVLLAFLERMRSDQFTGNWTSLQTAVISTP"
     gene            complement(594..1343)
                     /locus_tag="PKK76_18470"
     CDS             complement(594..1343)
                     /locus_tag="PKK76_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70519.1"
                     /translation="MNKALILAFCSFIPGLGCKTPQTQELPVQELSVRDRLEKIRAAY
                     APAPCFYASFGVESFQPGKSGQKADGTVRVDNANRRVRFYFTDSIFGITLSHMTIRDG
                     FVYIGSPRDPVQQIPVDRFVVGGLANNSIRLPFRLFEDLMYGRVPEQVFADKTHYRTE
                     GARLFAEYEDRESKSVYEFENDRMRSVTYLHKQDRANAKAEMTGTYPRSPFPQKMELN
                     GWIDGPPEKMIVNFQGVDMTAVCKEVQFPVN"
     gene            complement(1340..>2105)
                     /locus_tag="PKK76_18475"
     CDS             complement(1340..>2105)
                     /locus_tag="PKK76_18475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL70520.1"
                     /translation="PYRALLSQRDAMAGVPQDSPSMAEMQLELAKILSGNPINRDQEA
                     VSILDGLIANGNENALYHRALIHQSLGACQDANRDWLEIERMKGPQMEAAFLRGGCAE
                     SMGDFAEAEKAFREVYSKRSDLPLIQNSLAYVLSLQGKELQEARSLIEKAVAADSDDA
                     HFQDTYGWIYFQTGDHLRARYHLQFASRLLDEEGHRNAEVFDHLGHVYEALGQNERSL
                     YYFDSAYRILAEKAKRMPFEENLMKNIQSKMEKQKK"
BASE COUNT          489 a          550 c          496 g          570 t
ORIGIN      
        1 ctggcagttc tggtggccaa cggcacccga tccgcgcaga ttggaattgc cttcgcactc
       61 tgtgtaagtg cagctgaatt tttgttccgc accaatcgcc gtttgttttt tgcggccgcc
      121 attgctgcaa ccatgctcat ctctctggtc tatctgtatg gtgacttttc cggaagacag
      181 actgattttc aaagaccgat cctctggacc ggagccgggg ccattttcga aaaccatccc
      241 tggattggca ctgggcctgg aggattcgct tctgccattc tagagtggaa gaagacgttc
      301 aatctctccc atcctatgag ctggtactat gtagagagta ctccatcagg acatgcgcac
      361 agcgatctac ttcacctgtc ggcgatcgga ggaatccccg gctgcctttt ctttttgtcc
      421 atcgtagctt tgtctgttcg taccgtggct tcccagaaat cagaagcaat cctttcacgc
      481 tctgcactca ttgcattctt tgctgcaggc gtagctcagt gctactttca ggacgatgaa
      541 gtagttgtga tctactgggt tttgcttgcc tttctagaaa gaatgaggag cgatcaattc
      601 acagggaact ggacttcttt gcaaaccgca gtcatatcta cgccctgaaa atttacgatc
      661 atcttctcag gtggcccatc tatccaacca ttcaattcca tcttctgagg aaaaggggac
      721 cgggggtagg tgcctgtcat ctctgctttc gcattcgcac gatcctgctt gtgcagataa
      781 gtcactgaac gcatgcgatc attttcaaac tcgtaaacgg atttgctttc gcgatcttcg
      841 tattctgcaa acaatctggc gccttccgtg cgatagtgag tcttgtccgc aaacacctgc
      901 tcgggcacac gaccatacat gagatcttca aacaaacgga atggaagacg aatagaatta
      961 ttcgcaagcc ctcccaccac aaatcgatcc actggaattt gctgcactgg atctcttgga
     1021 ctaccaatgt atacaaagcc atcgcgaatg gtcatgtgcg aaagggtgat tccgaatatg
     1081 ctgtctgtaa aataaaaacg tacgcgcctg ttggcgttgt ccacgcgaac tgtaccgtcc
     1141 gccttctgcc cggatttgcc aggctgaaag gactccactc cgaagctggc atagaagcaa
     1201 ggtgcgggtg cgtatgctgc gcgaatcttc tccagccgat cgcgaacgga gagctcctgg
     1261 acaggaagtt cctgtgtttg aggagtcttg catcccagac caggaatgaa actacagaat
     1321 gccaggatga gagctttgtt cacttttttt gtttctccat tttagactga atgttcttca
     1381 ttagattctc ttcaaacggc attcgttttg ctttttccgc cagaatgcga taggcgctgt
     1441 caaaatagta cagggacctt tcgttttggc ccagagcttc atacacatga ccaaggtgat
     1501 caaatacttc cgcgttgcga tgtccctctt catccagcaa ccgggaggcg aattgcagat
     1561 ggtagcgtgc gcgcagatga tctccagtct gaaagtaaat ccagccgtat gtatcctgga
     1621 aatgcgcgtc gtccgaatct gctgccaccg ctttttcgat cagggatctg gcttcctgca
     1681 attctttgcc ctgtagactg agaacgtatg cgagcgaatt ctgaatcagc ggaagatctg
     1741 atcttttgga gtaaacctct cgaaaggcct tctctgcctc agcaaaatcg cccatggatt
     1801 ctgcgcagcc gccgcgtaaa aatgcggcct ccatttgtgg tcctttcatg cgttcaattt
     1861 ccagccagtc gcgatttgca tcctgacagg cacccagaga ttgatgaatc aaagcccggt
     1921 ggtaaagagc gttttcattt ccattcgcga tcaacccatc caggatactg actgcttcct
     1981 gatctcgatt gattggattg ccagacaata tcttcgcaag ctccagttgc atctccgcca
     2041 tagaaggaga atcttgcgga acaccagcca ttgcatcgcg ctgtgataaa agtgcacgat
     2101 aaggt
//
LOCUS       DAORFF010000632         2101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_41865_length_2101_cov_3.539101, whole genome shotgun sequence.
ACCESSION   DAORFF010000632 DAORFF010000000
VERSION     DAORFF010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41865_length_2101_cov_3.539101"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..856)
                     /locus_tag="PKK76_18480"
     CDS             complement(65..856)
                     /locus_tag="PKK76_18480"
                     /inference="COORDINATES: protein motif:HMM:NF039204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HNL70521.1"
                     /translation="MLKKILPLLTLLFGALVASGAGFYLVRVRETALAEYIKQAHQIE
                     REFVTRTPIYEDFATPDMEMTLRKYLLMDHLRVAEKAGVGAVATDEEVQKLVEEGKLV
                     PARPDEDADFHFYNVPKEGRFLTPRAREGLELIAQRFQKNIEKRYQKHPPVRIAVSSV
                     LRTTGYQKDLQKKNPNASFASSHSYGVSFDLFYDDYYVKLPPVSPGIPDELRTRLGYL
                     LGDALMRQFRAILMETLIQLQEEGEIYAILEKNQRCYHVTVLEKK"
     gene            complement(857..>2101)
                     /locus_tag="PKK76_18485"
     CDS             complement(857..>2101)
                     /locus_tag="PKK76_18485"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNL70522.1"
                     /translation="RSLFMNSLDRLITLALPDARIFAVFFLDLDRFKVINDGLGHIAG
                     DILLVEIARKLRAMIPPHCMVARLGGDEFTVLAENAADEREIIHIAETILREIKSPMM
                     VDGHEVYTTVSIGIALSTGDYRRPEEMIRDADTAMYQAKKQGKSRYQIFQAQMRARAL
                     EAVRVEADLRRALPGREFELFFQPIVRAQERQVIGFEALIRWNHPERGYISPLDFISV
                     AEETGLIVQMGEWVVREACKAAKRSLGGRYVAVNLSAVQLQQPHFVETIRSALIGAAL
                     PPELLLLELTESVLMESDPAALQALGILRDMGVSLAIDDFGTGYSSLSYLKKFPVNVL
                     KIDRSFVRETPADTSSCEITRVILSMCRILGLRVVAEGVENDAQLQFLSGEGCENIQG
                     FFFSRPVPEKNIDSVIRSISGV"
BASE COUNT          533 a          570 c          486 g          512 t
ORIGIN      
        1 tcttcgagct gttccgttag ccggtccacg ttcgactctc tttccatttc agcagcctgg
       61 caagctattt cttttccagg actgtcacat gatagcagcg ctggttcttt tcgaggatgg
      121 cataaatttc tccttcctcc tgaagttgaa tcagagtctc cattaaaatc gctcgaaact
      181 gacgcatgag agcatccccc aggagataac ccaatcgagt gcgtagctca tctggaattc
      241 cgggactgac aggaggaagc ttcacgtagt aatcgtcgta gaacagatca aagctgacgc
      301 cgtagctatg cgaagatgca aacgaagcgt ttggattttt cttttgcaaa tctttctgat
      361 agccagtggt gcgcaggaca gaagaaacag cgattcgcac tggaggatgc ttctggtatc
      421 tcttttcaat atttttctga aacctttgtg caatcaattc gagaccttcc ctggcgcgcg
      481 gagtcagaaa cctgccttct tttggaacgt tataaaaatg aaaatcagcg tcttcatcgg
      541 gacgcgctgg aaccagcttg ccttcttcca ccagcttttg cacttcttcg tccgtggcaa
      601 ctgcgccaac gcctgccttc tcagcgacgc gtagatgatc catgagcaag tacttgcgaa
      661 gagtcatctc catgtctgga gttgcgaaat cctcatagat aggagtgcgc gtcacgaatt
      721 cacgctctat ttgatgggcc tgcttgatgt actccgcgag cgctgtttcg cgcacgcgca
      781 caaggtaaaa acccgcaccg gatgccacta gagcaccaaa gagaagtgta aggagtggaa
      841 gaatcttctt gagcattcag acaccagaaa tcgatcgaat caccgagtcg atgttctttt
      901 cgggaactgg tctggaaaaa aagaatcctt gaatgttctc gcagccttca ccggacagaa
      961 actgaagctg cgcatcgttc tccaccccct ctgcgactac gcgaagccct agaatgcggc
     1021 acatggacag aatcactcgg gtgatctcac agctgctggt gtccgccgga gtttcacgga
     1081 caaaactgcg atcgatcttt aaaacgttta cagggaattt ttttaagtaa ctgagagaag
     1141 aatagccagt cccaaaatcg tctatggcca gagacactcc catgtcccga agaatgccaa
     1201 gtgcctggag tgcagccgga tcactttcca tcagaaccga ttctgtcaat tccaggagca
     1261 gcaactcagg aggaagggcc gcgcctatca gagcagaacg aatcgtttcc acaaaatgtg
     1321 gctgttgcag ttgaactgcg gaaagattca cggccacgta tcttccgcct aacgatcgtt
     1381 tggcggcctt gcacgcttcg cgcaccaccc attcgcccat ctgaacaatg aggccagtct
     1441 cctccgcaac gctaataaaa tccaatggag aaatatatcc tctctctgga tgattccacc
     1501 tgatcaaagc ttcaaagcca atcacttgcc tttcttgcgc acggacaatg ggttgaaaaa
     1561 ataactcgaa ttctcgaccg ggcaatgcac gtctgagatc cgcttccact ctgaccgctt
     1621 cgagcgcccg cgcacgcatc tgggcctgaa agatctgata cctggacttg ccctgtttct
     1681 ttgcctggta catggcagtg tccgcgtcgc gaatcatttc ttccgggcgt ctgtagtccc
     1741 ccgtactgag cgcaattcca atgctcactg tggtgtaaac ttcgtgtcca tccaccatca
     1801 tgggcgattt gatttcgcgc agaatggttt ctgcgatgtg gatgatttct ctctcgtccg
     1861 ccgcattctc cgcaagcact gtaaactcat ctccacccag acgcgccacc atgcagtgcg
     1921 gtggaatcat ggccctcagc tttcgcgcaa tctcaacgag cagaatatca cctgcaatat
     1981 gaccgagacc atcattgatc actttgaaac gatccagatc caaaaagaaa actgcaaata
     2041 tgcgcgcatc cggcaatgcg agagtaatga gacgatcaag cgaattcata aacagagatc
     2101 g
//
LOCUS       DAORFF010000633         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_42067_length_2095_cov_2.859804, whole genome shotgun sequence.
ACCESSION   DAORFF010000633 DAORFF010000000
VERSION     DAORFF010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42067_length_2095_cov_2.859804"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="PKK76_18490"
     CDS             complement(<1..1273)
                     /locus_tag="PKK76_18490"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNL70523.1"
                     /translation="MYKKLEIYKSDMKYKARTLSLLLFAVSWSPAMAADPVLLEAGRG
                     VYRLGPNLEILTDLEGKLTIDQITSGNYTSSFYGYGWDRVPNFGYSSATFWVRCRITN
                     PLPFQKEVLLELANPLLDEARLFFVDEGASSRTITESKSIKHRVYIFPVHVRPHETVD
                     LYLRVKSKGSIALPLTLFRPDAFAREDQDTQFALGIYYGLIAVMILYNLTLFIALRER
                     AYLYYSLALFCIHGMLQFAVNGLARKYLPLSASWWSLGSIPFFASLGIFWMLLFCRHF
                     LNTRLRTPEIHRVTTALLVVSLLMTIGSLFMPYRAAVTILTIHGLAGLFCVIAAGAYS
                     WRESFAPARYYLLGWSVVALGAFIYNIKSWGWLPSNPYTEYSAQSSLAIEAILLALAL
                     SDRFNTIRQEKFQAQQRALDQERMTREAEEEL"
BASE COUNT          492 a          476 c          620 g          507 t
ORIGIN      
        1 gaagttcttc ttctgcttcg cgcgtcattc gttcctgatc gagagctctt tgctgggcct
       61 gaaatttttc ctgtcggatt gtgttgaagc gatcactcag agcgagtgca agaaggatgg
      121 cctcgattgc caggctcgat tgtgcgctgt actctgtgta agggttgctt ggaagccatc
      181 cccagctttt gatattgtag atgaaagctc cgagcgccac tacactccag ccgagcaaat
      241 agtatcgcgc aggcgcgaat gattcccgcc agctgtaagc gccggctgca atcacgcaga
      301 acagtccagc aaggccgtgg atcgtaagaa ttgtgacagc tgcgcgatag ggcataaaaa
      361 gagacccaat cgtcatcaag agagatacaa ccagaagcgc tgttgtgact ctgtgaattt
      421 caggcgtgcg aagacgagtg ttcaaaaagt gtctgcagaa aagaagcatc cagaatatgc
      481 caaggcttgc aaagaatggg atggaaccca ggctccacca cgatgcagac aatggtagat
      541 acttgcgtgc caggccgttg actgcgaact gaagcatgcc gtgaatgcag aagagtgcga
      601 gagaataata gaggtaagcg cgttcgcgaa gcgcaatgaa cagagtcaga ttgtacagga
      661 tcatcaccgc gattaggcca tagtaaattc caagtgcgaa ctgagtgtcc tggtcttcgc
      721 gcgcaaacgc gtctggtcga aatagagtga ggggcagagc aatcgaccct ttgcttttta
      781 ctcgcaggta gagatctacg gtttcgtgcg ggcgaacatg caccgggaaa atgtaaactc
      841 tatgtttgat gctcttgctt tctgtgatgg tgcggctgga tgctccttcg tctacaaaga
      901 agagtctggc ctcatccagg agcgggttgg ccagttcgag aaggacttcc ttttgaaagg
      961 gaagcgggtt ggtgattctg cagcggaccc agaatgtagc ggagctgtat ccgaagttgg
     1021 ggaccctgtc ccagccatat ccgtagaagc tgctggtata attcccggat gtgatctggt
     1081 ctatggtgag cttcccttcc aggtccgtta gtatttcgag gtttggcccg agtctataca
     1141 ctccccgtcc tgcctcgagc aggactggat cagcggccat ggcaggactc caggataccg
     1201 caaacaggag aagagagaga gttcgggctt tgtatttcat gtcagatttg tagatttcta
     1261 attttttata catctagaat tctagacggc aggctctatt ccagcttttg gacaggcatt
     1321 ggctagcatt ttcttggagg gagagggagg ttctcgctct ttttcgtcgt tcttgggcca
     1381 agagcaaaca ggtttcttct atattatctt ctgaaatgtg acgattcagt gctgattact
     1441 acaaaaaaag aggcgttcat gcagggtgtg ctctgcctgg cacgctggtt gcaatagagg
     1501 gtctgtcaac gaagagtcat cgggcacctg cttgaggggg aaagagatgg atcgtcatct
     1561 ttgttcatcg caatcctctc ttccacagca gagatcgccg ggggcggtgg ttcttcagcc
     1621 agggcaatga agggctgaca gttgcctgga acattcggtc agggggagct cgggggagcg
     1681 ccggatggtc tgggacaacg aaaagcgccg gcgctcaggg ggcagctgat tggattcgaa
     1741 aggatccggg ggagtcagct gagagcgcgc actgttttgg atgacaggga accaccggat
     1801 gaatgccttc tggcattcgg gggccggtag cgccatcata ctgtgagtgg atcgtgcatg
     1861 gaccatcatg cggtctacag tgaaagcggg ggagtgagat cgggggtggc ggacgagagt
     1921 ccgcctttct tttttgtccg cccgctttgc ctgcgatccc agacgagccg ttgattccgt
     1981 ctaacgagct gtttgttcca ttggatgcac ttcctcatcg ttttagattg caggacaccg
     2041 cgctgagctc tggctatgtg cagaggtgta aattgaaaaa tccaaaaacg aaacg
//
LOCUS       DAORFF010000634         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_42247_length_2089_cov_3.470010, whole genome shotgun sequence.
ACCESSION   DAORFF010000634 DAORFF010000000
VERSION     DAORFF010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42247_length_2089_cov_3.470010"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PKK76_18495"
     CDS             complement(<1..693)
                     /locus_tag="PKK76_18495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNL70524.1"
                     /translation="MPKISIVIPALNEEQDLPHLLESIKNQTFTDYEVIVADAGSKDK
                     TIEIAKSYGAHVVPGGMPGPGRNRGAAAAKGEFLFFFDSDVILPHDFLQGALAEMQDR
                     FLDLATCEFKPDSDLRLDQLLFHLANLSVKVNQSFNPRAAGFCIFMTKRLFERVGGFD
                     ETLKLAEDHDIVQRASVFRPLRVLNNVELKVSVRRLKKEGRLSLIGKYMQVELHLLLK
                     GKVTEDIVEYEFG"
     gene            complement(766..1158)
                     /locus_tag="PKK76_18500"
     CDS             complement(766..1158)
                     /locus_tag="PKK76_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70525.1"
                     /translation="MKKLIPILSILLIGASALFAYSFEIKDVGYRKWSGDDCKAFLRK
                     NDGYCTSGDCKALLRANDGYCDTNDCKAVIRGNDGYCQSGDCKAILRKNDGYCETDDC
                     KAFLRKNDGYCQTDNCKAFLRNNDGYCR"
     gene            complement(1215..>2089)
                     /locus_tag="PKK76_18505"
     CDS             complement(1215..>2089)
                     /locus_tag="PKK76_18505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNL70526.1"
                     /translation="LFSDKRRSLFMGIWNASIPLGAATGVAVGGYVAQHYGWQRAFGL
                     VALPGLVVSLLFFFVKDYKTVKLSENQKHMGMLPIAKKFLATPSLILTYIAFAANNVV
                     TSALLVWLPTFLHRTQGIPMDQAGPKGGAVMLLAIVGAPLGGFLADLWHKKKPNARPV
                     FAGLSALVTTAVFWVAFSLFSHGSSAQYGMILVGGLVAVSYVPAGAALTQDVVHPGLR
                     AVSYSLCVVSMNMLGASVGPLLIGSISDHTDIATALSVLPAFSLVSALFFFAASKYYV
                     RDVNRAQSIENVAT"
BASE COUNT          533 a          516 c          521 g          519 t
ORIGIN      
        1 accgaattca tactctacaa tgtcttctgt gactttgccc tttaacagaa gatgcaactc
       61 cacctgcatg tattttccaa tcagagaaag tcgtccttct ttcttgagcc tgcgaacact
      121 tactttcagt tctacgttgt tgagaactct aagagggcga aacacagaag ctctttgcac
      181 tatgtcgtga tcttctgcga gtttgagagt ttcgtcaaaa ccacccactc gttcgaagag
      241 cctcttggtc ataaaaatgc agaatccggc tgcgcgtgga ttgaacgatt gatttacttt
      301 tacggaaaga ttggccagat gaaagagcag ttgatcgagt cttagatcgg aatccggttt
      361 gaattcgcag gttgctagat cgaggaaacg atcctgcatt tctgcaagtg ctccctgtag
      421 aaaatcgtgc ggaagaatta catctgaatc aaagaaaaaa agaaattccc cttttgcggc
      481 ggccgctccg cgatttcgac ctggtccagg catgccgcct ggcacaacat gagcaccata
      541 gctcttcgca atttcaatgg tcttgtcttt agatcccgcg tccgcgacaa ttacttcgta
      601 atcagtgaaa gtctggtttt ttatactttc tagaaggtgg ggcaggtcct gttcttcgtt
      661 cagtgctgga atgacgatgc tgattttggg cataggcatt caggggccgg gccctgccgg
      721 gatacaagga aaaaaagaaa gccttggtac ccggccggcg gtagtttagc ggcagtagcc
      781 gtcgttgttt cgcagaaagg ccttgcaatt gtctgtctgg cagtatccgt cattttttct
      841 gaggaatgct ttgcaatcgt ctgtttcgca atagccatcg ttcttgcgca agatcgcctt
      901 acagtcacct gactggcagt aaccgtcatt tccacgaatc acagctttac agtcgtttgt
      961 gtcgcagtaa ccgtcgttcg cgcgcagtag agccttgcaa tctccggacg tgcagtagcc
     1021 gtcgttcttt ctaaggaatg ctttgcaatc atctccggac cacttgcgat agcctacatc
     1081 tttgatttca aaagaatatg cgaagagcgc acttgcgcct atcagcaaga tggatagaat
     1141 gggaatcaac tttttcataa cgttatttga cgaccggttt gccagttcgt ccatgaaaaa
     1201 atcacgcggt gacgttacgt cgcgacgttt tctatggatt gggcgcggtt cacatctcgt
     1261 acgtagtact tgcttgctgc aaagaagaac agagccgata ccagagaaaa cgcaggcaat
     1321 acagaaagag cggtggcaat gtccgtgtga tccgaaatgc ttccaatgag caaaggcccg
     1381 accgatgctc ccagcatgtt catggaaacc acgcaaaggc tgtaagaaac agcgcgaagg
     1441 ccagggtgga ctacgtcctg agtcagcgcc gctcccgcag gtacatagga cactgcgacc
     1501 agtcctccca ctaagatcat accgtactga gcggagctac cgtgcgaaaa caaactgaac
     1561 gcgacccaga aaaccgcagt ggtgacgaga gcggaaagtc cagcgaatac aggtctcgcg
     1621 tttggtttct ttttgtgcca gagatccgcc agaaatccac cgagaggcgc gcccacgatg
     1681 gcaaggagca tcacagctcc tccttttggc cccgcttgat ccatgggaat tccctgagtg
     1741 cgatgcagga atgtgggaag ccagacgagc agagcactgg tcacaacgtt gtttgccgca
     1801 aatgcaatgt aggtcagaat gagacttgga gttgcgagga attttttcgc aatgggtagc
     1861 atgcccatgt gtttttgatt ttcgcttagc tttacggtct tgtaatcctt cacgaaaaag
     1921 aacaaaagtg aaacaacgag gcccggcaga gccaccagtc caaacgcccg ttgccatccg
     1981 taatgttgcg ccacatatcc acctacggcg actccagtgg cggctcccag tggaatggat
     2041 gcattccaga ttcccataaa gagggatctt cgcttgtctg aaaaaagac
//
LOCUS       DAORFF010000635         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_42327_length_2087_cov_3.233760, whole genome shotgun sequence.
ACCESSION   DAORFF010000635 DAORFF010000000
VERSION     DAORFF010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42327_length_2087_cov_3.233760"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..847)
                     /locus_tag="PKK76_18510"
     CDS             complement(80..847)
                     /locus_tag="PKK76_18510"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNL70527.1"
                     /translation="MQGLLDRIVAAKREEIRSLKSIQPARVESHFLAAVKRAPGDSVR
                     VIAECKKTSPSSGRMRETYEPEKIATEYQRLGASALSVLTDRQFFEGDPAHVAMAKKS
                     GLPALRKDFVIDEKQIADSAVLGADAVLLIVRILEVSQLADYIAYADSLGLPALVETH
                     NEKEIEIAMRVKSAIIGINHRDLDTLKMDLSLTPRLAPVVRRELPGCVIVAESGVESR
                     DGLKTVDEFVDSVLIGTAFMKSQNVASIWQEIFGGAR"
     gene            complement(849..1175)
                     /locus_tag="PKK76_18515"
     CDS             complement(849..1175)
                     /locus_tag="PKK76_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL70528.1"
                     /translation="MLNTKKVGDKMVVYLEGRLDVSVANDVEEELNRIIETDNQKHLV
                     LNMEKVEYMSSSGFRACISTLRKLNAKDGTLKLCSIRPSVKRIFDVIELTSLFDICET
                     EADALK"
     gene            1256..>2087
                     /locus_tag="PKK76_18520"
     CDS             1256..>2087
                     /locus_tag="PKK76_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNL70529.1"
                     /translation="MEKRKTILVIDAHAMAYRAHFALERQNLTNAAGQNTGAIFGFFR
                     MFFKILQDFTPEETLVAWDPPGGGFRGDMYKEYKATRKPMPDDLRSQISEIKEIVRNM
                     GFATCEVPGFEADDVMGSMATRFGKASDVLLLTSDKDCFQLITKNVSMLKGAKGVTEF
                     VRVDPAWVENEIGVRPDQIPEYMALVGDTSDNIPGAKGIGPKTAAELIQQYKSIDGIY
                     KNLPRIAKESVRKKLQESESDVRLSRDLATIRTDIKEVLDFPEAKLRTPDYLREDVVQ
                     F"
BASE COUNT          485 a          527 c          540 g          535 t
ORIGIN      
        1 ttcatgagcc tggctgcgtc tgccacagta gcgttccctt ccaggcacgc cagatcccgg
       61 atcattactt gcgtgattct catctggctc ctccgaagat ttcctgccat atagaggcca
      121 cattctgtga tttcataaat gctgtgccga tcagcaccga gtccacaaac tcatctacgg
      181 ttttgagtcc gtctctggat tccacgcctg attcggccac aatgacacat ccgggaagct
      241 cgcgtctcac tacaggtgcg aggcgcggtg tcaggctaag atccattttt agagtgtcca
      301 ggtctctgtg attgattcca atgatcgcgg attttactct catcgcgatt tcgatttctt
      361 tttcattgtg agtctccaca agcgcgggca agccaagaga gtctgcgtat gcaatgtaat
      421 ctgcaagctg tgagacttct aaaatgcgaa cgatcagtaa gactgcatct gctccaagca
      481 cagctgagtc tgcgatttgt ttttcatcaa tgacgaagtc ttttcttagc gcaggaagtc
      541 cgctcttctt cgccatagcc acgtgcgctg gatccccttc aaaaaattgt ctgtccgtaa
      601 gtacggagag agcggatgca cccagtctct ggtattcggt tgcgatcttt tctggttcgt
      661 aggtttcgcg cattctaccg gaggaggggc tggttttttt gcactctgcg atgacgcgca
      721 cggaatcgcc gggagcacgt ttgactgcgg ccaggaaatg gctctccact ctggctggct
      781 ggatggattt gagtgagcgg atttcctctc gcttggccgc cacgatgcgg tctagcaggc
      841 cctgcacgtt acttcagtgc gtctgcttcc gtttcgcaaa tgtcaaagag gctggtcaat
      901 tcgatgacat caaagatgcg ttttacggaa gggcggatgg agcaaagctt cagagtgccg
      961 tctttggcat tcaatttgcg caatgtagaa atgcatgcgc ggaatccgga ggagctcatg
     1021 tactccactt tttccatatt caggaccaga tgcttctggt tgtccgtttc aatgatacgg
     1081 ttgagttctt cttctacgtc gttggcgacg gaaacgtcca gacggccttc gagatagaca
     1141 accatcttgt ccccgacttt ttttgtattg agcattcagg gcacctctaa acagtactcg
     1201 gggcaccttg cattgccagg cccgccctgt caagcatcct gatagccagg aaaagatgga
     1261 aaaacgcaag accattctag taatcgacgc tcacgcgatg gcataccgtg cgcatttcgc
     1321 gctcgagcgt cagaatttaa ccaacgctgc cggtcagaat acaggcgcga tttttggatt
     1381 ctttcggatg ttctttaaga tccttcagga ttttaccccg gaagaaactc tcgtagcctg
     1441 ggatccgcca ggtgggggat ttcgcggcga catgtacaag gagtacaagg caacgcggaa
     1501 gcctatgccg gatgatctca ggagtcagat ttctgaaatc aaagagatcg ttcgcaacat
     1561 gggttttgca acctgcgagg tacctggatt tgaagcagac gacgtgatgg gaagcatggc
     1621 tactcggttc ggcaaggcgt ccgacgttct gcttcttacc tctgacaaag actgttttca
     1681 attgatcaca aagaacgtca gtatgctcaa gggagcaaaa ggcgttacag aattcgtgcg
     1741 cgtggatcct gcatgggtgg aaaacgaaat cggtgtgcgc cccgatcaga tcccagaata
     1801 catggcgctc gtcggtgata cgagcgacaa cattccaggc gcaaagggca ttggccccaa
     1861 aacagccgcg gaactcatcc agcagtacaa atccattgat ggcatctata aaaatctccc
     1921 gcgcattgcg aaggagtcag tcaggaagaa attgcaggag agtgaatcag acgttcgtct
     1981 ttctcgcgat cttgccacca ttcgcacgga catcaaggaa gtgctcgatt ttcctgaggc
     2041 aaagcttcgc acgccggatt atctgcgcga agatgtagtt caatttt
//
LOCUS       DAORFF010000636         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_42537_length_2082_cov_3.555501, whole genome shotgun sequence.
ACCESSION   DAORFF010000636 DAORFF010000000
VERSION     DAORFF010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42537_length_2082_cov_3.555501"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="PKK76_18525"
     CDS             complement(<1..1050)
                     /locus_tag="PKK76_18525"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HNL70530.1"
                     /translation="MASKKKQPEIVLPDVSFFGIRHHSPRSSSALFALLEKMRPECIL
                     VEGPTDARPLFEAVAASDTLPPVAILGYRTDDQVGSSFYPLASYSPEYVAIRWAFANK
                     VDFDFIDIPFEQSIAYDRHQKEDPSEDMEPENNSALFYKTLADRFGCRSFEEFWEAQF
                     EGPSYDPDTFRAVIVAYSDVSRQFGADLDFHRARDAFMAARIQDKIQGGANPSRTLVV
                     AGAAHVAAFMAKDVAPEKIDLLPAACPVAVTLIPYSFTRLAEQTGYGAGNRAPRYYER
                     AYQRGCDFKAASLEMLIEFCSHLRLRGFAVSLADTIEAYRLANMLAQMRNKSAPGLDE
                     VRDAAAATLCRGELSH"
     gene            complement(1053..>2082)
                     /locus_tag="PKK76_18530"
     CDS             complement(1053..>2082)
                     /locus_tag="PKK76_18530"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNL70531.1"
                     /translation="LIAGDKSEKPPGWAMTPRAAETYLMGSDKPVGGVKITPKYIGDA
                     SIIQVAIATLASDRALMLTGEPGTAKSWISEHLSAAISGTSAYVIQGTAGTSEEHIKY
                     GWNYAMLLAEGPSHKSLVASPILRGMKEGKFARFEEITRTSSEIQDALISILSEKQIA
                     IPELGEMIHAKRGFNIIATANTRDRGVNEMSAALKRRFNFVNIPIVDDLEQEIQIVLK
                     RESELRSDYSVGAQPPEELGKVLVTVFQELRKGITRDGKIKIKSPNSVLSTAEAISVL
                     FNGGILAQSFGSGKVTGAEIARALVGAVAKESQEDLKVLKEYCETVVKTRTGPWKEFY
                     KAATEKLN"
BASE COUNT          453 a          536 c          544 g          549 t
ORIGIN      
        1 atgactgagt tcaccgcggc atagagtcgc ggcggccgca tcgcgtacct catctagccc
       61 gggggcagac ttgtttcgca tttgagcgag catgtttgcc aggcgatacg cttcgatcgt
      121 atctgccagg gacacagcga atccacgcaa gcgaaggtgg ctgcaaaatt cgatcagcat
      181 ctcaaggctg gccgctttaa aatcgcagcc gcgctggtag gctctttcat agtacctggg
      241 cgctctattt cccgcaccgt accctgtttg ttctgcaaga cgcgtgaagc tgtaggggat
      301 caaggtgact gcgacggggc aggctgcggg aagcagatcg attttctccg gcgctacgtc
      361 ttttgccata aatgcagcaa cgtgagccgc tcctgccacg acgagtgttc tggatgggtt
      421 tgcgccgccc tggattttgt cctggattct cgcggccatg aaagcatcgc gtgcgcgatg
      481 aaaatcaaga tccgctccga attgccggct aacgtctgaa tatgcgacta tgactgcgcg
      541 aaacgtgtcc ggatcatagg atggtccttc aaactgggct tcccagaact cttcaaacga
      601 tctacatcca aaacgatctg caagtgtttt gtagaataaa gcagaattgt tttctggctc
      661 catgtcttca gatggatctt ctttttgatg tcgatcgtag gcaatggatt gttcgaatgg
      721 aatgtcgatg aagtcaaagt caactttgtt ggcgaacgcc cagcgaattg ccacatactc
      781 gggagagtaa gaggcgagcg ggtaaaaact cgagccaacc tgatcatctg ttctatatcc
      841 aagaattgca acgggcggca aagtatcact ggcggccact gcttcgaaca aaggtctcgc
      901 atccgtcggt ccctctacca gaatgcattc ggggcgcatc ttttcaagga gtgcaaagag
      961 tgcgctcgag ctgcgcggag aatggtgccg aattccaaaa aatgaaacgt ccggcagcac
     1021 tatttcaggc tgcttctttt ttgaggccat gatcagttca atttttcggt tgcggcttta
     1081 tagaattcct tccagggacc agtccgcgtc ttgacgactg tttcgcagta ctctttcagc
     1141 accttcaggt cttcctgaga ttctttagcg acagctccga ccagggcgcg cgcaatttct
     1201 gcgcccgtga ccttgccgga tccaaaactc tgcgcaagaa tgccgccatt gaataaaaca
     1261 gaaattgctt ctgctgtaga cagaactgaa tttggtgatt tgatttttat ttttccgtct
     1321 ctagtgatgc ctttgcgcag ctcttgaaag actgttacaa gcactttgcc gagttcttca
     1381 gggggctggg ctcccacgga ataatcggat ctcagttcgg actctcgttt tagaacaatt
     1441 tggatttcct gctccaggtc atctacaata ggaatgttta cgaaattgaa acgtcgcttc
     1501 aaggccgcgg acatttcatt gacgccgcga tctcttgtgt tggctgttgc gatgatattg
     1561 aatccgcgtt tagcatggat catttccccc agctcgggga tggcgatttg cttttctgaa
     1621 agaatcgaaa tcagagcgtc ctgaatctcg gaactggtcc gagtgatttc ctcaaagcgt
     1681 gcgaactttc cttctttcat tccgcgcaag attggagacg caaccaggga tttgtgcgaa
     1741 ggcccttctg ccaggagcat tgcgtagttc cagccatact tgatgtgttc ttcgctggtg
     1801 cctgccgtgc cttgaattac gtatgcgctc gtccctgaga tcgcggcaga caaatgctct
     1861 gaaatccagc ttttcgcagt acccggctct ccggtcaaca tcagggcgcg gtcggaggca
     1921 agggttgcga tcgctacctg gatgatggaa gcgtccccaa tgtatttggg agtgattttg
     1981 accccaccga ctggtttgtc gcttcccatc aaatacgtct cggcggcccg cggcgtcatg
     2041 gcccaccctg gaggcttttc gcttttgtcc cctgcaatga gt
//
LOCUS       DAORFF010000637         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_42931_length_2072_cov_3.299455, whole genome shotgun sequence.
ACCESSION   DAORFF010000637 DAORFF010000000
VERSION     DAORFF010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42931_length_2072_cov_3.299455"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1670
                     /locus_tag="PKK76_18535"
                     /pseudo
     CDS             1..1670
                     /locus_tag="PKK76_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004767629.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
     gene            complement(1873..>2072)
                     /locus_tag="PKK76_18540"
     CDS             complement(1873..>2072)
                     /locus_tag="PKK76_18540"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNL70532.1"
                     /translation="AVTLDSGLLYVAEALRVPTISLWGTHAPAVRLGYDPAYMDLAIW
                     PRENCGAAPCFAYANFPYASL"
BASE COUNT          591 a          509 c          549 g          423 t
ORIGIN      
        1 tcaagcgaat ccttcgcgaa gtgcaggcat ccggaaacat catcatgttc attgatgagc
       61 tccatacctt gattggtgcg ggtgccgcag aaggcgccat tgatgcagcc aacatgctca
      121 agccagctct ggcgcgtggt gagattcaat gcgtgggtgc tacaacactc acagagtatc
      181 gtaagtacat cgaaaaagat tcggcactcg aacgtagatt ccaggcaatc catgtaaaag
      241 agccttcgat cgaagataca attttgattc tgaaaggtct cagaaaagca tacgagacac
      301 atcatcgtgt gcgttacccg gatgatgtca tcgagctcgc agttcgactg tcggagagat
      361 acatcacgga cagacatctg ccagataaat ccattgatat catcgatgaa tctggatcac
      421 gggctagact ggccaactca gttcgtcctg cagagatcac agatctcgaa gatgagatca
      481 aagctctcgt tcaaaaaaaa gacgacatgg tcaaggcgca agaatacgaa aaagcagcgc
      541 aaatccggga tgaaatcaac actcgcaaag aagtgcttga agaaaccact cacagatggc
      601 aagagagaat cgcggatttc tctgtagaga ttcgcgcaga agacatctac tcagtcgtga
      661 gcctgtggac tggaattcca ctcgagcaaa ttgaagaagg ggaagcggac aagcttctca
      721 agatggatca agagcttgcc cgtcgtgttg tgggccagaa agaggcagtg gatcttgtga
      781 gccgtgccgt tcgcagatcg cgcactggtt tcaaagattc gcgcaagcca agcggatctt
      841 tcattttcct cgggccaacc ggagtaggaa agacagaact ggctcgcgca ctcgcagagt
      901 tcctgtttgg aaacgaggca aagcttttcc gtctggatat gtccgaatac atggagccgc
      961 acagtgtttc caagctgatt ggttctcctc caggatacgt gggttacgat gatgcagggc
     1021 agctcagtga gtttgttcgc aggaatccgt actctgtggt tctgctcgat gaaatcgaaa
     1081 aagcgcatcc ggatatcttc aatcttctgc tccagattct ggaagaagga actctcacgg
     1141 atactcacgg acgcaaagta gacttccgcg aaaccatcat cattatgacg tcaaacctgg
     1201 gtgcacgcga aatgcacaag ggcggcaaga tgggcttcgc cgaagatgat tcacgtcgtg
     1261 aacagaacaa gaaagagaag ctggtcgaag aacttaagaa gcattacaat cctgagttcc
     1321 tcaatcgcat cgatgaaatc gtgactttcc acacgcttac gcgtgagaat atctacgaaa
     1381 tcatcgacat catggttaag cgcatgaacg atcatctatt cgagaagagg atcaagctgg
     1441 agctggacga ttccgctcgc gaattgatca cgaagctcgg gtatgatgaa aagtatggtg
     1501 caagaccact tcgtcgtgtt ctacagcgcg aaattgaaga tcacatgtct cagcgtttac
     1561 tggaaggtgc gttcaaagaa ccaaccattc tgcacgtatc cgctgaaact atggacgatc
     1621 agacaaagct caaatacgca gaggcttcct gggtcgacta tgaaacggtc aaggaagaga
     1681 agcgcgtgga gacggaacgc aaggaagcag cgcgcaagga agcagagagg aatcgcattc
     1741 ggtcccgctc ggttcttccc ggcgaacccg ttccacccaa accagattta atccccggac
     1801 aagcatgaaa aagacagcaa ctctcctcat tctattcgca gccgtagcac ttttcgcaga
     1861 ggtgccaaac ggctacaatg aagcatacgg aaaattggcg taggcaaagc acggcgctgc
     1921 gccgcagttc tcacgcggcc agatcgccag gtccatataa gccgggtcgt agcccagcct
     1981 aaccgccgga gcgtgggtgc cccacagcga gatggttggg acgcgcaggg cctcggccac
     2041 gtacagcagg ccggagtcaa gggtgacggc ca
//
LOCUS       DAORFF010000638         2069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_43042_length_2069_cov_3.611221, whole genome shotgun sequence.
ACCESSION   DAORFF010000638 DAORFF010000000
VERSION     DAORFF010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43042_length_2069_cov_3.611221"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /gene="fliF"
                     /locus_tag="PKK76_18545"
     CDS             complement(<1..1069)
                     /gene="fliF"
                     /locus_tag="PKK76_18545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNL70533.1"
                     /translation="MPEGLKNVLDRITQLWTGLDNTKKLAIGGVLAAVVIAFIAMAGY
                     SSKPSKELLYDNLSATDYASISKALEAMGVEYSGSGTSAIYVDGHRRQEIVTKLAQEN
                     LIPAGVEGWEIFNMSRWDETSFDKDVKLHRAIKGSLEKMLMTLEYVKSARVELAIPKR
                     NNFLTDTDPVKASVILSLKPGMESITRKQVMGVKNLIYRSIPGLTRENITITDQNGKE
                     FVEPDEIDEAQRKLDLVDRKKAFEEKERRKWYDEIKTSLNEFYQADRVSIVRVALDMN
                     WDEVTEKQHLVTPVEESPENPDTPYPDRKIMPGGTLTLSQNARKENFKGNGFTPGGPT
                     GTEQQLPPGYRDLDYQRSEYGN"
     gene            complement(1228..1611)
                     /locus_tag="PKK76_18550"
     CDS             complement(1228..1611)
                     /locus_tag="PKK76_18550"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNL70534.1"
                     /translation="MNIQSERGVQFSGGMPQFRPSNRVPLGDAKLTTQDPRHMQIGPQ
                     GPESARSTGPAKGFADMLSRALEDVESVDSRSRDLTTKAIYDPDSVEVHDVMIAAEKS
                     RFALTLTKTVADGLVRTFRELTQPR"
     gene            complement(1648..>2069)
                     /gene="flgC"
                     /locus_tag="PKK76_18555"
     CDS             complement(1648..>2069)
                     /gene="flgC"
                     /locus_tag="PKK76_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNL70535.1"
                     /translation="AQRLKLDVISNNIANVTTTRNAQGDGPFRRDRVVMEPINQRSVW
                     RSPVYPRGLETGPGQGVKVAKIEKDMRPLRLVYDPTHPDAIAIGPKKGYVEMPNVNIV
                     EEMTDMIAASRSYEANLQVVQGARQMFNRALEISRAV"
BASE COUNT          442 a          534 c          503 g          590 t
ORIGIN      
        1 gattgccata ctcggaacgc tggtagtcca gatcgcgata cccaggaggg agctgctgct
       61 ctgttccagt cggcccacct ggagtaaatc cattgccttt gaaattttct ttgcgggcat
      121 tctgagaaag agtcagagtt ccgcctggca tgatcttacg atctggataa ggtgtatccg
      181 ggttttctgg tgactcttcc actggagtca ccaggtgctg cttttctgtt acttcatccc
      241 agttcatatc gagagcaacg cgaacgatgc tcacgcgatc tgcttgataa aattcattga
      301 gagacgtttt gatttcgtcg taccatttac ggcgctcttt ttcctcgaac gctttcttgc
      361 gatccactag atcgagtttg cgctgcgctt catcgatttc gtctggttct acgaattcct
      421 ttccgttttg atctgtgatc gtgatgtttt cacgagtcag acccggaata gaacgataga
      481 tcaagttctt cacgcccatg acctgtttgc gcgtgatgct ttccatgcca ggcttcagag
      541 agagaatcac ggaagctttg actggatccg tatccgtaag gaagttgttg cgctttggaa
      601 tcgccagttc tacgcgagcg ctcttcacat actcaagagt catgagcatt ttctcgagag
      661 aacctttgat cgcgcgatgg agtttgacat ctttgtcaaa gctcgtttca tcccagcgag
      721 acatgttgaa aatctcccaa ccttctactc cggctggaat caagttctcc tgagcgagtt
      781 tggtgacgat ttcctgacga cgatgtccat cgacgtagat ggcagaagtg ccgcttccgc
      841 tgtattctac tcccattgct tcgagtgcct ttgaaatact tgcgtagtca gtggcactca
      901 gattgtcata gagcagttct ttggacggtt tggacgaata tcctgccatt gcgatgaatg
      961 caatgacgac ggctgccagc acgcccccaa ttgcaagctt cttggtattg tccagcccgg
     1021 tccagagctg agtgattcgg tcgagaacgt tttttaaacc ctccggcatg gtacaccttc
     1081 ctgtatttgt tgcttccaag agacataacg tgccagaaag tacaagaaaa attttataga
     1141 aaccagggaa tttttataaa ttttctgtac gtacggcgaa gacgccgcgc caccctttcg
     1201 ggcgcgcgga ttctctttaa ggttggatta tcttggctgt gtgagttcgc ggaatgtgcg
     1261 gaccaggcca tctgcgactg tctttgtaag agtcagtgca aatctgctct tttctgcggc
     1321 gatcatcaca tcatgaactt ccacagaatc tggatcgtag attgcttttg tagttagatc
     1381 cctggatcta gaatccacgg attcaacatc ttccagagcg cgagaaagca tgtccgcgaa
     1441 gcctttggca ggtccagtgc tgcgcgcgga ctcaggtccc tgcgggccga tctgcatgtg
     1501 acgcgggtcc tgggtagtaa gctttgcgtc ccccagagga acacgattcg aaggacgaaa
     1561 ctgcggcatg ccgcctgaaa actggactcc gcgttcactc tgaatattca tcgcttaact
     1621 ccctgaagtt gatctatttt tattttctta tactgctctt gagatttcga gtgcacggtt
     1681 gaacatctgc cgcgcaccct ggactacttg caaattcgct tcgtaagaac gagaggcagc
     1741 aatcatgtcc gtcatttctt ccactatgtt tacatttggc atctctacgt agcccttctt
     1801 cggcccaatc gcaatcgcat ccgggtgagt cggatcatac acaaggcgca gagggcgcat
     1861 gtctttctcg atttttgcta ccttcacacc ctgccccggt ccggtctcga gacctcgcgg
     1921 atacactggc gatctccata cgctgcgctg gttgatgggt tccatcacca cacgatcccg
     1981 gcgaaaaggt ccatctccct gggcattgcg cgttgtagtt acgtttgcta tgttgttcga
     2041 aatcacgtcg agcttcagtc tctgcgctg
//
LOCUS       DAORFF010000639         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_43503_length_2056_cov_3.200900, whole genome shotgun sequence.
ACCESSION   DAORFF010000639 DAORFF010000000
VERSION     DAORFF010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43503_length_2056_cov_3.200900"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..792
                     /locus_tag="PKK76_18560"
     CDS             133..792
                     /locus_tag="PKK76_18560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL70536.1"
                     /translation="MSRLDQRVTETLSRLHRDAEADKFVFARSIPAALGAKLRGRSVF
                     QAVAPRLKDAYMPVTPQMGEFLHITALQLHAKTIVEFGSSFGISAIYLSSALPPGGRF
                     IGSEMEPNKVSQARRNLESAGLADVAEIRPGDALETLARPMDQPIDLLFLDGWKDLYV
                     PILKLLTPRLRSGSVVLADNIYTFRRELAEFVATVSNPDNGFASMTLPFKSGLQYSVR
                     I"
     gene            890..>2056
                     /locus_tag="PKK76_18565"
     CDS             890..>2056
                     /locus_tag="PKK76_18565"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNL70537.1"
                     /translation="MRIVLLLCFLLTGLVQAREPLTLDDFYTHLSYGNVKQVREYMQA
                     HRVDVNALAGPGECEGLPLLQCAGSAGVMEILLQNGARIDQVTNGQTALDHAASEKDG
                     GEAARFLLKKGAKPSGRTLHAAIWYENTELAYELIDMGVDVNYRDADQGYTPLLIACE
                     NGRPLSLIKKLIERGAKLDVVTNEGFAALHFFPSKRSTDHQGGAGDTTIGEEERLEKT
                     TYLLSKGAKLDVKTKEGFTPLHIAVQSGDEIAMLLIEKGANIQAVSNTGATMLQLAAG
                     AGSARLVKYFLDHGANVQAKNGEGYTAIFYGAQSGSGEVVKLLLDKGAVCTDYGKDRK
                     TPLFLIAMFPTAEAHYETLEENPQDFYTALTLVLQKSGPALIHSPDIYGETPWFL"
BASE COUNT          525 a          472 c          565 g          494 t
ORIGIN      
        1 aagaagaaaa aaacataaaa gaccacgcac caccagaagg aatacttctt gccaaatcat
       61 taggtaggct aattattagg tatactaacc attaggagtg ggtgtatgcc tgttttaaat
      121 gaaagtcggg tcatgtcccg gttggatcag agagttacgg aaacactgtc gcgtttgcat
      181 cgcgacgcgg aggcagacaa gttcgtgttc gcaaggtcga ttccggcggc acttggcgca
      241 aaacttcggg gaagatcggt atttcaggct gtagcaccac gattgaagga cgcttacatg
      301 cctgttactc ctcagatggg agagttcttg cacataactg cgcttcaact tcatgcaaag
      361 acgattgtag agtttggaag ttcttttggg atttcagcga tttatctttc ttccgcgttg
      421 ccgccaggtg ggcgtttcat tggtagcgaa atggaaccaa acaaagtatc tcaggcgcgg
      481 cgcaatctgg agagtgctgg acttgcggat gtagctgaaa ttcgtcctgg cgatgcgctg
      541 gaaacactgg ccaggcccat ggatcagcct attgatctgt tgttcctcga tggctggaaa
      601 gatttgtatg tgccaatcct gaaacttcta acgccgcgac tcagatcagg ttctgttgtt
      661 cttgcggata atatatacac tttcaggcga gagcttgcag aatttgttgc aacggtgtcc
      721 aatccggaca acggattcgc gtctatgacc ttgcctttca agagcggatt acagtattca
      781 gttcgcatct gagcccatag atgccgcata acgtaatgcg gcattgtttt tttgattgcg
      841 cgaatgttgc cgcggtttac actgaagcag gaatgcttcc aggatgacta tgcgaatcgt
      901 actactactc tgttttctcc tcacaggcct ggtgcaagcc agggagccac tgactctcga
      961 tgatttctat actcatctga gctatggcaa tgtgaaacag gtccgcgagt acatgcaggc
     1021 ccatcgagtg gatgtaaatg ctctggctgg accaggcgaa tgcgaaggat tgccgctgct
     1081 gcaatgtgcg gggtccgccg gagtcatgga gattcttctt caaaacggcg cgagaattga
     1141 ccaggtcacc aacgggcaga cggccctgga ccatgctgct tctgagaagg acggaggaga
     1201 ggcggcgcgc tttctcttaa agaaaggtgc gaagccttcc gggcggacac tgcatgcagc
     1261 catctggtac gaaaacacag agctcgctta tgaactgatt gatatgggag tggatgtgaa
     1321 ctaccgggac gcagatcagg gatacactcc gctcctcatt gcatgcgaaa acggaagacc
     1381 cttgtccttg attaagaaac tcatagagcg cggcgcaaaa ctggatgttg ttacaaacga
     1441 aggttttgct gctctgcatt tttttccgag caagcgatcc acggatcatc aagggggagc
     1501 gggagacacc acgattggag aagaggaacg tcttgaaaag acaacgtatc ttctgtcaaa
     1561 gggcgcgaag ctggatgtga aaacgaaaga aggattcacg ccccttcaca tcgccgtgca
     1621 gtctggtgat gaaatcgcaa tgttgctaat tgaaaaaggt gccaacatcc aggcagtgtc
     1681 gaatacgggc gccaccatgt tgcagcttgc cgcgggcgca ggatccgcca ggctcgtgaa
     1741 atattttctg gatcacggag caaacgtcca ggcaaagaac ggggaagggt acactgccat
     1801 tttttatggg gctcaaagcg ggagcggaga ggtagtgaag cttcttcttg ataagggcgc
     1861 tgtgtgcacg gattacggga aggatagaaa gactccgctc tttctcatcg caatgtttcc
     1921 aacagcagaa gcgcactatg aaactctgga agagaatccg caggattttt atacggcact
     1981 caccctggtg ttgcagaaaa gcggtccggc gcttattcat tctcccgaca tttacggcga
     2041 aaccccatgg tttctt
//
LOCUS       DAORFF010000640         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_43979_length_2044_cov_3.116139, whole genome shotgun sequence.
ACCESSION   DAORFF010000640 DAORFF010000000
VERSION     DAORFF010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43979_length_2044_cov_3.116139"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..518)
                     /locus_tag="PKK76_18570"
     CDS             complement(87..518)
                     /locus_tag="PKK76_18570"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNL70538.1"
                     /translation="MDIVSLDGAFAYYINRTYRSLRMDFLDLTRNAGYELTPEQYFIL
                     NKLFHRPGQSQSELAAELNDRANIARGLAILQRKKLIRRSKNSEDKRKFSVQLTKDGE
                     AVLKKLNPVIEARRKVVYSGLTGKDLQTLRHILTKIESNLD"
     gene            680..1042
                     /locus_tag="PKK76_18575"
     CDS             680..1042
                     /locus_tag="PKK76_18575"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNL70539.1"
                     /translation="MEQGIRDSIEQFVRAGDEQDRDLFLKVTHANFRVVAPNFPAPGS
                     IAFLERDQFASLLQSKQIGGEVRKIDFLGWEPIGPSAATVRTRLTGTKLRFENAFALI
                     KMEDKWQIVQDMAAASAG"
     gene            1000..1467
                     /locus_tag="PKK76_18580"
     CDS             1000..1467
                     /locus_tag="PKK76_18580"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNL70540.1"
                     /translation="MADCSGHGCCVCWMMLREVNIDSLYETKSRGFSQAVIVEMRPPA
                     MIQSGVGFISGMVAWDTDRRIPQDIDIGEETTLAMQNVVRLLRAESREVQSIVQVRTF
                     VVNLTPELARTVSRALEVFFTETAHRPASTMVGVTGLAAPEVRVEIEVVFSTH"
     gene            1619..>2044
                     /locus_tag="PKK76_18585"
     CDS             1619..>2044
                     /locus_tag="PKK76_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70541.1"
                     /translation="MRNLKYVIFSFGLSILFSGSLLAQSVAGPGGNYWPLFCKTGPNA
                     KVEVDDTSNLRIFFESSGKAAGNNFAGVTPGTCSYPDRPPRKDELQGYYGEIFAQWHC
                     PALIGTCLIKYDSDSHTYKGNFPWLRDPAGRKFKMWVRKD"
BASE COUNT          488 a          465 c          539 g          552 t
ORIGIN      
        1 gatgaactgc cagaaagact atgtcaacgc agcaggcgtg aaatgcgtct ggcagtgcgt
       61 gagatcctac tcggacgcta acttgtctaa tccaggttcg attcgatctt tgtcaggatg
      121 tgtctcagag tctggagatc cttgcccgtt agtccagaat agacaacctt gcgtcttgcc
      181 tcgatgacgg gattcagttt cttcaagacc gcttccccat cttttgtgag ctgcacactg
      241 aactttcttt tgtcctcaga attcttgctc ctccggatca gcttctttct ctgaagaatt
      301 gcaaggcccc gcgcgatgtt cgctcgatca ttcaattctg ccgccagttc cgattgcgat
      361 tgtccaggtc tgtgaaatag cttattcaaa ataaagtact gttcgggtgt gagttcataa
      421 cccgcatttc tggtgagatc cagaaaatcc atccggagag atcgataggt gcgattgatg
      481 taataagcaa acgccccgtc caggcttacg atgtccacgg tcccaaatgg tggacgcttc
      541 tggggtttgc cacccaaaaa accaggccgg aacattcgtc tgaatctttt tttgttgcat
      601 aagatacatt tcgcggctat attggtgcat atgcaacatt atgttcgaat gagcaatgtt
      661 gtaaaaaatg gaggcacata tggaacaagg aattcgcgat tcgattgagc agtttgtcag
      721 ggcaggagat gagcaagacc gggatctctt tttgaaagtg acccatgcaa attttcgcgt
      781 ggttgcgccc aattttcctg ctccgggaag cattgcattt ctggagcgcg atcagttcgc
      841 aagtttgctg cagtcaaagc agattggcgg agaagtgcga aagattgatt ttcttggctg
      901 ggagccgatc ggtccgtctg ccgcaacggt aagaacgcgt ctgaccggaa caaagcttcg
      961 gtttgagaat gcattcgcac tgatcaagat ggaagataaa tggcagattg ttcaggacat
     1021 ggctgctgcg tctgctggat gatgttgcgc gaagtgaaca ttgactcttt gtatgaaaca
     1081 aagagtcgtg gtttttccca ggccgtcatt gtagaaatgc ggccgcctgc aatgattcag
     1141 tcgggcgtgg gattcatttc tggtatggtc gcatgggata cagaccgacg cattccacag
     1201 gacatcgaca ttggtgaaga gacaaccctt gccatgcaaa acgttgtgcg ccttcttcgt
     1261 gcggaatcaa gagaggttca aagcatcgtt caagttcgca cttttgttgt gaacctgact
     1321 ccggagttgg cacgaaccgt gtcgcgtgca cttgaagttt tcttcactga aaccgcgcat
     1381 cgtcctgcat ccacgatggt cggagtgacg ggacttgcgg ctcctgaagt tcgagtcgaa
     1441 atcgaagtag tcttttcaac tcattgatgg agttttcgtc gcaactctgg aatgttccaa
     1501 cggatccatg ggccactgtt gtggcgagat cggttctctt ccgggagtag gtgagatgtt
     1561 cgttttttca tgaagaaata ctgttgaaat cggcatctgt gcatggtttg gtggaaggat
     1621 gagaaacttg aaatatgtaa tattttcttt tggcctgagc atcctgttca gtggttctct
     1681 tctcgcacaa tctgtggccg gtccaggtgg caactactgg cctctgtttt gtaaaacggg
     1741 tccgaatgcg aaggtagagg tggatgacac atccaatctt cgcatcttct tcgaaagttc
     1801 tggaaaggct gccggaaata actttgcagg cgtgactccg ggcacatgca gctatccaga
     1861 tcggccgccg cgcaaagacg aactgcaggg ttactacggc gaaattttcg cgcagtggca
     1921 ttgccccgcg ctcataggaa cctgtttaat caagtacgac tccgacagtc atacgtataa
     1981 agggaacttt ccatggctta gagatccagc cgggcgcaaa ttcaaaatgt gggttcgcaa
     2041 ggac
//
LOCUS       DAORFF010000641         2043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_44019_length_2043_cov_3.176559, whole genome shotgun sequence.
ACCESSION   DAORFF010000641 DAORFF010000000
VERSION     DAORFF010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44019_length_2043_cov_3.176559"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1844
                     /locus_tag="PKK76_18590"
     CDS             <1..1844
                     /locus_tag="PKK76_18590"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNL70542.1"
                     /translation="DQSRFVFRKNTRPISNSSWIDRLPTAASVISSRAEAMKQERISL
                     SPGPAVIDLVQVPIMNLEPAALSEEVTHTESVDDFVSTEEAEDAGELSVDHPSLSEPS
                     VAHEQMRSIEKVEEETEAELEEDSTDDATQTLEYLPTGVPSIRRRGGRYSLPTDILNL
                     SNPIPVQDAADEVKETTEKLEKVLRDFGIPGSVVRTQRGPIITLFEVLLDSGVRLNKI
                     LGIEGEIRMHLSVPSVRIVAPLPGKPTVGLEIPNRVRQTVTLGELARKDRDFFSKHRL
                     GIVLGKDISGKNLYLDLTRAPHLLIAGASGQGKSVYLNSAIASLLYKYSPEEVRFIMI
                     DPKMVELKLYEGIPHLLLPVITDPRQAARALAWIIQEMEYRYAHLSRVKCRDILSYNE
                     RMKTQGKTERIPFIVIVIDELSDLMLIAPKEVEDALWRLSQKARAVGIHMIMATQRPS
                     VDVITPHIKANAAARVAFHVAQKVDSRIILDQAGADALLGGGDMLYKSPDSTGITRVQ
                     APLITEEEIASIVQETRRYGEAVFIELGTEEEEETMEDDGSVDAELLDQAWKIILESG
                     KPTTSYIQRRMRIGYNRAANIIETLEMKGYLGPAIGTSRREILRKQP"
BASE COUNT          519 a          507 c          530 g          487 t
ORIGIN      
        1 cagatcaatc tcgttttgta tttagaaaga ataccagacc catttccaat tcctcatgga
       61 tcgatcgcct gccaactgcg gccagtgtga tctcttctag agcggaagcc atgaagcagg
      121 agcgcatttc tctgagcccg ggcccggctg tgatcgatct ggtgcaggtt cccatcatga
      181 atttggaacc agcggctctc agcgaagaag tgacgcatac agaatctgtg gatgattttg
      241 tttctacaga agaggcagag gacgcaggcg agctttctgt ggatcaccca tctctcagtg
      301 agccctccgt cgcacacgaa cagatgcgtt ccatcgaaaa agtcgaagaa gagactgagg
      361 ctgagctcga agaagactcc acggatgatg caacgcaaac tctcgagtat cttcctactg
      421 gagttccttc gattcgcagg cgcggcggca gatactctct tcccacagat attctgaatt
      481 tgtctaaccc cattcccgtg caggatgcag cggatgaagt aaaagagact acagagaagc
      541 tagaaaaggt tcttcgcgat ttcggaattc ctggaagcgt ggttcgcacg cagcgcggtc
      601 ctatcatcac gttgtttgaa gtgcttcttg attctggagt tcgtttgaac aaaattctgg
      661 gaatcgaagg ggagattcgc atgcatctct ccgtgccatc tgttcgcatt gtagcgccac
      721 ttccaggtaa accaaccgtt ggtcttgaaa ttccaaatcg agtgcgccag accgtaaccc
      781 tgggcgagct tgcgcgcaaa gacagagact tcttctccaa gcacaggctc ggaatcgttc
      841 ttggaaaaga tatttcagga aagaatctct atctggatct cactcgggcg cctcatcttc
      901 tgatcgcagg ggcttccggg cagggtaaat ccgtgtatct caactccgcc attgcatctc
      961 ttttgtataa gtacagcccc gaagaagttc gtttcatcat gattgacccg aagatggtgg
     1021 agctcaaact ctatgagggt attccgcacc ttctcttacc agtcattacg gatcccagac
     1081 aggcagcgcg cgcgcttgcg tggatcattc aggaaatgga ataccgttat gcgcatttgt
     1141 ccagagtgaa gtgccgcgac attctttctt acaatgaacg catgaaaact caggggaaaa
     1201 cagaaagaat tccatttatt gtgattgtaa tcgatgagtt gtcagacctg atgctgattg
     1261 ctcccaaaga agtggaagat gctctgtgga ggctttcgca aaaggcacgt gcggttggaa
     1321 ttcatatgat tatggcaaca cagcgtccat ccgtggatgt gatcactcct catatcaagg
     1381 cgaacgctgc cgcgcgtgtt gcatttcacg tggcccaaaa agtagattct cgcatcattc
     1441 tcgatcaggc tggcgcggat gcacttctgg gcggtgggga catgctgtat aagtcgccgg
     1501 actctactgg catcactcgt gtgcaggctc cactcatcac tgaggaagaa atcgcaagca
     1561 ttgttcagga aacccgcaga tacggagaag cagtgttcat tgagctgggt accgaggaag
     1621 aagaagagac catggaagac gatggcagcg tggatgcaga gcttctggat caagcctgga
     1681 agatcatcct cgaaagcgga aagccaacca caagctacat tcagagaagg atgcgcattg
     1741 gatacaaccg cgctgcgaat attatagaaa ctttagaaat gaagggctat ctgggccctg
     1801 cgatcggaac gagtcgccgc gaaattctaa gaaagcagcc gtgatcgcgc atctgagaat
     1861 cggcccgttt tgtagagcgg gcgcgaaaat ctattcggag cacacggatg ctttgccgtc
     1921 tgtgtccgat tcttctgcaa accagacgac tgcgtagccc ccatacattc caaccccgac
     1981 cgattgccag ggatgactgc cccacttgtc tttgttcagg atcacattgt tgtggcctga
     2041 gct
//
LOCUS       DAORFF010000642         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_44240_length_2038_cov_2.379728, whole genome shotgun sequence.
ACCESSION   DAORFF010000642 DAORFF010000000
VERSION     DAORFF010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44240_length_2038_cov_2.379728"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKK76_18595"
     CDS             <1..283
                     /locus_tag="PKK76_18595"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNL70543.1"
                     /translation="HDRDGGYSTKVHEVNQFGIAIVLVGNFEKHSVPDQQMEALLHLI
                     LRLKREHGIPLDHIVGHRELWATACPGKHLNMSEVRAKIAEMEKNDAHR"
     gene            267..1886
                     /gene="nadB"
                     /locus_tag="PKK76_18600"
     CDS             267..1886
                     /gene="nadB"
                     /locus_tag="PKK76_18600"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNL70544.1"
                     /translation="MTRIVDTDYLVIGSGISGLYSALILSEFGKVTIITKEEVTDANT
                     TYAQGGIASVLAASDTFESHIRDTLDAGAGLCDTEAVRVLVTEGPTHIRRLMELGAAF
                     DQDASGSLTLGREGGHSENRIVHAKDLTGKEVEDVLLRAVRARNIEIKEYQSAVDLIT
                     CYHLEKDPYLPGVAPPGPNQCFGAYVYDRKTWEIYIIRARATILATGGAGQVYLHNTN
                     PDVATGDGVALAYRAGAVISNMEFYQFHPTALYSPGHRTFLISEAMRGEGGILRGPDG
                     EPFTKKYDERGDLAPRDIVARAIDAEMKRTGAAFMHLDVTHLPREQIMKKFPNIYAHC
                     LDLGIEISRDRIPVVPAAHYMCGGVRTDVNGRTAIEHLFAVGEVSCTGVHGGNRLASN
                     SLLEGLVFADRIGRYIKENQISGQRPEIRRWQKEGLANAEEWILVQHNLGEIKSVMWD
                     YVGIVRSNLRLDRALRRIEMLSNEINDFYSRTVIQNKILELRNLALVGKLIVRSAKAR
                     RESRGLHFSTDYAENREPSMAYTSLQRSSAG"
BASE COUNT          533 a          459 c          553 g          493 t
ORIGIN      
        1 gcatgataga gatggcggtt actctacaaa agtgcacgaa gtgaatcaat ttggcattgc
       61 cattgtgctc gttggaaatt ttgaaaagca ttccgttcca gatcagcaga tggaagcatt
      121 gcttcatttg atcttacgat tgaagagaga gcatggaatt cctctcgatc acattgtggg
      181 gcacagagag ctatgggcca ctgcctgtcc gggcaagcat ttgaatatgt ctgaagtgag
      241 agcgaagatc gcagagatgg aaaaaaatga cgcgcatcgt tgatacagac tacctggtca
      301 ttggaagtgg aatctctgga ctctactctg cgttgatact cagcgaattt ggaaaagtca
      361 ccatcatcac aaaggaagaa gtaacagacg caaacaccac ttacgcgcag ggcggcatcg
      421 caagcgtgct tgcagcttct gatacgtttg aaagccacat tcgcgatact ctggacgcgg
      481 gtgcaggact gtgcgatacg gaagcggtcc gcgtccttgt tacggaaggt ccgacccaca
      541 ttcgcagact catggaactg ggcgctgcct ttgaccagga tgcgagtgga agtctcaccc
      601 ttggacgcga aggaggtcac agtgaaaatc gaattgtcca tgcgaaggat ctcaccggca
      661 aagaagtaga agatgttctt ttacgtgctg tgcgagcgcg aaacatagag atcaaagaat
      721 accagagcgc tgtggacttg atcacctgct atcatttaga aaaagatccg tatcttccag
      781 gtgttgcacc gcctggcccc aatcaatgct ttggcgcgta cgtgtatgac agaaaaacct
      841 gggaaatata tatcattcgc gcccgggcca cgattctggc aacaggcggt gccggccagg
      901 tttatctgca taacacgaat cctgacgtgg ccacaggaga tggcgtggct ctcgcgtaca
      961 gggcaggcgc tgtgatctcg aatatggagt tctatcagtt tcatccgact gcactttatt
     1021 cgcccggtca caggactttt ctcatcagcg aagcgatgcg cggagaaggc ggaattctgc
     1081 gtggaccgga cggagagcct ttcacaaaga aatatgacga aagaggggat cttgcaccca
     1141 gggatattgt agcccgggcc atcgatgcgg agatgaagag aacaggtgcg gctttcatgc
     1201 acctggatgt gactcatttg cctcgcgagc aaatcatgaa gaagttccca aacatctatg
     1261 ctcattgtct ggacctgggg attgagattt cgcgcgatcg catcccggtg gttccagcgg
     1321 ctcactatat gtgcggagga gttcgaacgg atgtgaatgg aagaacagcc atagaacatt
     1381 tgttcgcagt gggtgaagtc tcatgtacgg gagtgcacgg tggaaatcgc cttgcatcca
     1441 acagtcttct agaggggctg gtgtttgcgg atcgcattgg acgttatatt aaagaaaatc
     1501 aaatatctgg acagaggcct gagattcgcc gctggcaaaa agaaggcctg gcgaatgcgg
     1561 aagaatggat tttagtccag cacaacctgg gtgaaatcaa aagcgtcatg tgggactacg
     1621 ttggaattgt gagatctaat ctcagactgg atcgcgccct tcgcagaatc gagatgctct
     1681 ccaatgaaat caatgacttc tacagccgca ctgtaatcca gaataagatt ctggagctgc
     1741 gaaatcttgc tctggtgggt aagctcattg tacgatctgc gaaggcgaga cgcgaaagca
     1801 ggggcttgca tttttctaca gactatgcag aaaatcgtga accttctatg gcgtacacga
     1861 gtctccagcg aagttccgca ggctgacgct agaattttct tttcttgtag aatgcatagc
     1921 aacgtgcgtg ttgtggttgc gtgcgaacgt ttgttcttag aataagacgc catgcgtctt
     1981 tcgtttctga ttttactttt tcccgctaca gctctatttt ctgaaaccat actcatgc
//
LOCUS       DAORFF010000643         2037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_44258_length_2037_cov_3.566599, whole genome shotgun sequence.
ACCESSION   DAORFF010000643 DAORFF010000000
VERSION     DAORFF010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44258_length_2037_cov_3.566599"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..942)
                     /locus_tag="PKK76_18605"
     CDS             complement(208..942)
                     /locus_tag="PKK76_18605"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HNL70545.1"
                     /translation="MTKSLLLCLIWVSSLGAQTRELDRVLRTHVADGRVNYKALKDDP
                     LFERSVQRLAQVNPGTLKSQSERLAFWINVYNAFTLKLIVDHYPVASINDLHSGPGLI
                     VSTVIGKTVWKVWKFRIHERNYTLDEVEHSILRPMGDYRIHAAINCASRGCPPLRSEA
                     YTAEKLNEQLDDQMRCFLRPPYARYDSASNTLYVSKVFDWFAEDFEKQEPLPYALLPY
                     LPENVNPKTAASARIRTLDYDWNLNE"
     gene            complement(1035..>2037)
                     /gene="dctP"
                     /locus_tag="PKK76_18610"
     CDS             complement(1035..>2037)
                     /gene="dctP"
                     /locus_tag="PKK76_18610"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNL70546.1"
                     /translation="QSFALAAEPIEIRLASNLPDNSRMARDFKEWSAKVEERTGRRIS
                     FRFYFDGSQGDERDVIRKTKLGQLDGGIITSGGLAMIHSDFRALRAPLFFASDAEHDY
                     VVEKCTADFKRISSTAGFELVLWNDMGWVYWYSRQPIANANGLRQVKMWQWTEDTAIS
                     ALHRQLGVSGVPLGQVELLSALQTGKVDAYYGTPLQSVEMDWYTQTRYSTSVPVAYEP
                     GALMISNRVWNRISPADRNVILSLGSTLQAELRRDIRKWNDDATSAMARRGVSVTQAE
                     SAFRSDLSEKAEVVYRTLSENAGSQTMYDLVKRHRSDYRRRHLSPGRDKEPFVPQSR"
BASE COUNT          487 a          543 c          503 g          504 t
ORIGIN      
        1 cttgcacccg ctggatgcaa cagcaaaccg aaagcgcaca caagcggggt tcgcattcat
       61 ccagggaaag agggaagact ggtttgggca atcgtttttg atctgcggtt tttgcaaact
      121 tgctcccata acaacagttc gtcgtcagca ggtttctgtt ttttaaccac cgttagtcga
      181 tcattcagtg cggttagagg gaaattccta ctcattcaaa ttccagtcgt agtccagagt
      241 gcgaatgcgc gcagaggctg ccgttttcgg attcacgttt tctggcagat atggcaatag
      301 tgcatacggc aaaggttcct gtttctcaaa atcctccgca aaccaatcaa agacttttga
      361 aacatacaac gtattggacg cactgtcgta acgggcgtaa ggtggcctga gaaaacagcg
      421 catctggtcg tcgagttgtt catttagttt ctcggctgtg tatgcctccg aacgcagagg
      481 cggacaaccg cgtgatgcgc aattgatggc agcatggatc cgatagtcgc ccatgggtct
      541 gagaatcgag tgctccactt cgtcgagagt gtaatttctt tcgtggatcc tgaacttcca
      601 gaccttccaa actgttttcc caatgaccgt agagacaatg aggccgggcc cactgtgcag
      661 atcattgatg cttgcgaccg ggtaatggtc tacgatcagc ttcagtgtga atgcattgta
      721 cacattgatc cagaacgcga gccgctcgct ctgcgatttg agtgtacccg ggtttacctg
      781 cgccagcctc tgcacagatc gctcgaagag cgggtcgtct ttgagagctt tgtagttgac
      841 gcggccatcc gctacgtgag tacgaagcac ccggtcaagt tcgcgggttt gcgcgccgag
      901 cgatgagacc cagatcagac agagcaacaa gctttttgtc atggcagtca tcctgtcttc
      961 tttccccact caagaagata ccagcactgc aaagggtaaa catttttact ccaatgattc
     1021 gtgtttccag gacgctagcg cgattgtggc acaaagggtt ctttgtctct tcccggagac
     1081 aaatgtctcc tgcgatagtc gcttctgtgc cgtttcacaa gatcatacat ggtctgcgat
     1141 cctgcgtttt cggaaagcgt gcgatacaca acctccgcct tctccgaaag atcagaacga
     1201 aatgcagatt ctgcttgcgt gacgctcaca ccgcggcggg ccatcgcaga tgtcgcgtcg
     1261 tcattccact tacgaatatc acggcgcaac tcggcctgaa gagttgaacc cagagagagg
     1321 atcacattgc gatccgcagg cgaaatacga ttccaaactc tattcgaaat catcagggct
     1381 ccaggctcat acgcaacggg aacactggtc gaatatctgg tctgtgtata ccaatccatt
     1441 tccactgatt gaagaggagt gccgtagtac gcatcgacct taccggtctg gagtgcggac
     1501 agaagctcca cctgacccag agggacgcca cttactccca gttgtctgtg caaagcgcta
     1561 atcgctgtat cctccgtcca ttgccacatc ttcacctggc gcaatccgtt ggcgtttgcg
     1621 attggctggc gagaatacca gtagacccat cccatatcgt tccacagaac caactcgaag
     1681 ccggcagtac tggaaattcg tttaaaatcg gcggtgcact tttccaccac gtagtcatgc
     1741 tcggcgtcgc ttgcgaagaa aagtggcgca cgcaatgcac gaaaatcaga atggatcata
     1801 gcaaggccgc ctgacgttat gattcctcca tccagttgac ccagttttgt cttcctgatc
     1861 acatctcgct catctccctg agaaccatca aaatagaatc tgaaggagat tcgcctgcct
     1921 gttcgttctt caacctttgc actccattct ttaaaatccc tggccattcg agagttgtcg
     1981 ggaaggttgg acgccaggcg aatctcgatt ggctcggccg ccaatgcaaa agattga
//
LOCUS       DAORFF010000644         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_44296_length_2036_cov_2.995962, whole genome shotgun sequence.
ACCESSION   DAORFF010000644 DAORFF010000000
VERSION     DAORFF010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44296_length_2036_cov_2.995962"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PKK76_18615"
     CDS             complement(<1..858)
                     /locus_tag="PKK76_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70547.1"
                     /translation="MGLKYYERFTPRVSYRETQDGKLITESFDSMGVVRDSELKTFRW
                     APSEKNEVLSGTLRLTRNKPVYLERFELVLAADEKKSPFVSLHDDISPDSGLRLFQHG
                     YHSWSWTGARQADETDNICLLKWKHNLDENPETQINSALPFSLPINVFPRKGKFHSEF
                     LVLLETNTKFLKKKNGKCILFSTYGRGDQFLKFRIHLDPKTAKLEEFAIVWDGAGMHM
                     PGSTIITLTQVKHFNSIHVRPRTRVRLKRTQPSPAEFLESCAKDVAGEFKTPKMENSV
                     TGWCSWYYYY"
     gene            complement(910..>2036)
                     /locus_tag="PKK76_18620"
     CDS             complement(910..>2036)
                     /locus_tag="PKK76_18620"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HNL70548.1"
                     /translation="LIVDDDPDIRAILQNILAENHNLLHANDGIEGLGVARKSLPDLI
                     ISDVMMPGMDGFEFCASIRSDPFLRRIPILLLTGEHDLGSKLKGLHQRADDYVTKPFH
                     AEEIEARVSNLLEKRRLERELQQRLEEMQNDLALARNVQQQLLPVRIPPLPNAHFETL
                     YRPIAALGGDFFEFVVLPDRLGLFICDVAGHGIPAALVASMVKIVLANVAMDAAGPGD
                     LMVRLNRQMIQSTGERYVTAFYATLDLSTLQMQFASAGHIAPILVRNGALIPLEAHGP
                     LLGAFPKDHWGEEVVALQPRDRVFFYTDGVIDCRNKSEEHFGEERLNSCLLAHQGATA
                     RFFLDQLYHEISHFVGDRNFADDITAFVIDLATRKGAVVS"
BASE COUNT          496 a          493 c          499 g          548 t
ORIGIN      
        1 gtaatagtaa taccacgaac accatcctgt gacagagttt tccatctttg gtgttttgaa
       61 ttcgcctgcc acatcctttg cacacgattc cagaaactct gcaggagaag gttgtgttct
      121 tttcagtctc actcgagtgc gaggccgcac gtgaatgctg ttgaagtgtt ttacctgagt
      181 gagcgtgatg atggtgcttc cgggcatgtg catgcctgcg ccgtcccaca caattgcaaa
      241 ctcttccagt ttcgcggttt taggatccag atgaattctg aatttgagaa actgatctcc
      301 tcttccgtag gtggagaata gaatgcattt tccatttttc tttttcagaa actttgtgtt
      361 ggtctcaagc aacaccagga actccgaatg gaatttccct ttccgcggaa atacgttgat
      421 aggcaaagag aatggaagtg cactattgat ttgcgtttcc ggattctcat ccagattgtg
      481 tttccacttt agaagacaga tattgtctgt ttcgtctgcc tgtcttgcac ccgtccagga
      541 ccatgaatga tagccatgct gaaacaatcg caggcctgaa tctggagata tgtcatcgtg
      601 cagggaaaca aaagggcttt tcttttcatc tgccgcgagt acgagttcaa acctttctag
      661 atacactggt ttgttgcgag tcaggcgaag agttccgctc aatacttcat ttttttcaga
      721 gggagcccag cgaaaggtct tgagttcaga atcgcgaacg actcccatcg aatcaaaaga
      781 ttctgtgatg agtttcccat cctgtgtttc acgatagctt acgcggggag taaatcgttc
      841 gtagtacttc agacccatct gcacagagga cgccaggcca tgcctgttgt aaagaatttg
      901 gcgggcgcgt cagctgacta cagcgccttt cctggtcgcc agatcaatga caaaagctgt
      961 gatgtcgtct gcgaagttgc ggtccccgac gaagtgagat atttcatgat acaattgatc
     1021 gaggaagaat cgcgcggttg caccctgatg ggcgagcaga catgaattca gtctctcctc
     1081 tccgaaatgt tcttcgcttt tgtttctgca atcgatcact ccatccgtgt aaaaaaatac
     1141 ccggtcgcgc ggttgcagcg ccacaacttc ttctccccag tgatcttttg gaaacgcgcc
     1201 cagaagtggt ccgtgtgcct ccagtgggat gagagctcca tttcgcacta gaatcggtgc
     1261 gatgtgcccg gcagaagcga attgcatctg tagagtgctc agatcgagtg tagcatagaa
     1321 agcagttaca tatctttcgc ctgtgctctg gatcatctgg cgattgagtc gaaccatcag
     1381 gtctccgggg ccagcggcat ccatggcaac attggcaaga acgattttca ccatgctggc
     1441 cacgagtgcg gcaggaatgc cgtggcctgc gacgtcgcag ataaaaagtc caagtcgatc
     1501 cggcaaaact acgaattcaa aaaaatcacc acccagtgcg gcgatgggac gatataacgt
     1561 ttcaaaatga gcgttgggca gaggcgggat tcgcacgggg agcagctgct gctgtacatt
     1621 tctcgcaaga gcgagatcgt tttgcatttc ttcaagccgt tgctgcaact ctcgttccag
     1681 tcttcgtttc tcaagcaagt tggaaacacg cgcttcaatc tcttctgcgt ggaaaggctt
     1741 ggtaacgtag tcatccgctc tctgatgaag acctttcagt ttgcttccaa ggtcatgttc
     1801 gcctgtcaaa aggagaattg gaattctacg aagaaatgga tctgatcgaa tgcttgcgca
     1861 aaattcaaat ccatccatgc ctggcatcat cacatcagag atgatcagat ccggcagact
     1921 ctttcgcgca acacccagtc cctcaatgcc atcgtttgca tggagcaggt tgtgattttc
     1981 tgctagaatg ttttggagga tggcacgaat gtccggatca tcatctacga tcaaca
//
LOCUS       DAORFF010000645         2035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_44336_length_2035_cov_3.570202, whole genome shotgun sequence.
ACCESSION   DAORFF010000645 DAORFF010000000
VERSION     DAORFF010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44336_length_2035_cov_3.570202"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2035)
                     /locus_tag="PKK76_18625"
     CDS             complement(<1..>2035)
                     /locus_tag="PKK76_18625"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNL70549.1"
                     /translation="KLFEHLHNAEYFERFLATRFPGKKRFSLEGGESLIPTLSALVEI
                     SGGFSVEQIIIGMAHRGRLNVLTNVMGKDPAMIFAEFNEKVTNDVGPGDVKYHLGYSS
                     DAVTSTGKKIHLSLGFNPSHLEVINPVVLGSVRARQTNGKDIERTQNLPLVIHGDAAF
                     AGQGINYECLQMSSLEGYTVGGAFHIICNNQVGFTTNPADARSTRYCTDLAKMLQVPI
                     FHVNGDDPEACYRAVQLCMEWRQRYGTDVFLDIVCYRRWGHNETDEPTFTQPVMYDAV
                     KNHPTTVTLYEQRLLQEGIPAEEVEAIKKKSRDHFEEAYQRFQGGGVEVQFETLKGKW
                     TGFTRINPSEPVTAVAEDELRAVADILTRAPGGFSVHPKIQKLLEGRRAMLAPGGKID
                     WGMGEALAYGTLLRQGYPIRISGQDVKRGTFSHRHAVLFDVKTDEEYAPLAQTAQKGA
                     HLQIYNSLLSEVAVLGFEFGYSLADPHSLVIWEAQFGDFANGAQVIIDQFICSCEAKW
                     SRMSGLVMLLPHGYEGQGPEHSSARLERYLQLCSLNNIIVANCTTPAQFFHIIRRQML
                     RNYRKPLIIMSPKSLLRLPEATSSLSELTSQAFDPVIDETDPSIDPAKVKRVVFCTGK
                     LYYELSAERRKQGVNHVALVRVEELYPYPVKEISSVIQRYPNAREFAWAQEEPR"
BASE COUNT          453 a          509 c          502 g          571 t
ORIGIN      
        1 cgcggctctt cctgggccca ggcaaattca cgcgcatttg gatatcgctg aatgacgctt
       61 gagatttctt ttacggggta gggatacagt tcctctactc gcacaagtgc gacgtggttc
      121 actccctgct tcctgcgttc tgctgacagc tcatagtaaa gtttgcctgt gcagaacaca
      181 actctcttga ctttcgccgg atctatggat ggatccgttt cgtctatgac tggatcaaac
      241 gcctgcgatg tgagttccga taaactgctg gttgcttccg gcaatctcaa aagagatttc
      301 gggctcatga tgatcagagg cttacgatag tttcgtagca tctgacggcg gatgatgtgg
      361 aagaactgcg cgggcgttgt gcaattcgca acaatgatgt tgttcagtga acacaattgc
      421 aaataacgtt cgagtcttgc gcttgaatgc tctggaccct ggccttcata gccatgcgga
      481 agaagcatca caagcccgct cattctggac cattttgctt cgcatgaaca gatgaactga
      541 tcgatgatca cctgcgcgcc gtttgcaaaa tcaccaaact gcgcctccca gatgacaagg
      601 ctgtgtggat ctgcgagaga atagccaaac tcaaatccaa gaactgcgac ctcagaaagc
      661 agactgttgt agatctgaag atgggcgcct ttttgtgctg tctgcgcaag cggtgcatat
      721 tcttcatctg ttttgacgtc gaatagaact gcatgtctgt ggctgaaagt tccgcgcttt
      781 acatcctggc ccgaaatacg aattggatat ccctggcgaa ggagcgtgcc gtaggcgagt
      841 gcttcaccca tgccccagtc aatctttcca cctggcgcga gcatcgcgcg acgaccttcg
      901 agtagcttct gaattttagg atgaacagaa aatccgcctg gtgcgcgagt gagaatatct
      961 gccactgcac gaagttcgtc ttctgctact gcagtcacag gttctgatgg attgatgcga
     1021 gtgaatccag tccatttgcc tttcaaagtt tcaaattgaa cttcaacacc gcctccttga
     1081 aaacgctggt atgcttcttc aaagtggtcg cggctcttct tcttgatggc ctctacttct
     1141 tctgcaggga ttccttcctg gaggaggcgc tgttcgtata acgttactgt ggttggatga
     1201 ttctttacag catcatacat caccggctgg gtgaaggttg gttcgtctgt ttcattgtgg
     1261 ccccatctgc ggtagcacac tatgtcgaga aacacgtccg ttccgtatct ctgtctccat
     1321 tccatacaca gctgcaccgc acgatagcat gcttccggat catccccatt gacatggaag
     1381 attggcacct gaagcatttt cgcgaggtcc gtgcagtaac gagtggatct tgcatctgcg
     1441 ggatttgttg taaatccaac ctgattgttg cagatgatgt ggaaagctcc gcccacagtg
     1501 tacccttcca ggcttgacat ttgcaggcat tcgtagttga ttccctgtcc ggcgaatgct
     1561 gcatctccat gaatcaccag aggcaaattc tgagtgcgtt ctatatcttt tccgtttgtt
     1621 tgtcgtgcgc gcacagatcc caggacaact ggattgatga cttcgaggtg gctgggatta
     1681 aacccgagac tgaggtggat tttttttcct gtagatgtga ccgcgtcgct ggagtagccc
     1741 agatggtatt ttacatcccc tggtcctacg tcgttggtta ctttttcgtt gaactccgcg
     1801 aagatcatgg ctggatcttt tcccatcaca ttggtcagaa cgttcaatcg tcctcggtga
     1861 gccatgccta tgatgatttg ctctacgctg aatccgccag aaatttccac aagagcagat
     1921 aacgttggaa tgagactttc tccaccttct agtgagaaac gcttctttcc cggaaaacga
     1981 gtggccagga atctttcaaa gtattccgcg ttgtgcaggt gttcgaataa cttct
//
LOCUS       DAORFF010000646         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_44720_length_2026_cov_2.789954, whole genome shotgun sequence.
ACCESSION   DAORFF010000646 DAORFF010000000
VERSION     DAORFF010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44720_length_2026_cov_2.789954"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="PKK76_18630"
     CDS             complement(<1..1514)
                     /locus_tag="PKK76_18630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNL70550.1"
                     /translation="MKRFRTVRFLILALAVSFALSSLFVAYAAARTVRMGRLTRESRI
                     KIAERLAFLESAGEVYFRTTNIEPLRAAMISFASETDLSVLFASDVDGKIIASTNIQD
                     VGELVANRYPRVHAKFLEDPLASGPRILPDAGTDFVVGYAGMCAPGRNGLRGGRCGFV
                     YYELDLAYRIRETNRELRTQMTVTAIGLLAGAFALWLVLTMRVTRRTETISTGIKKFA
                     SGNRSVRLRLEGADEIAELGRDVDDLFARVNETEIALKDRESRLREVFDSLLDGLIII
                     DTRGTIQSINPAGSAIFGYRDGELAGQNISVLMPEPHHSMHDVYLANYVATGVKNVIG
                     MAREAEACRKDGTKFPIDLKVSELRAEGQLLFIGIIRDISERRQMQQDLIAANQELEH
                     AVTQLAQAATTDSLTGLYNRRSLDVRIEEEIRRAQRHGQPVTLLVCDVDHFKMFNDHY
                     GHQAGDDCLIAISNILQSHFRRGGEFTARYGGEEFVVILPDLTLSEGVERAEHLR"
     gene            complement(1511..>2026)
                     /locus_tag="PKK76_18635"
     CDS             complement(1511..>2026)
                     /locus_tag="PKK76_18635"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNL70551.1"
                     /translation="KMTSADITIVPIRINEHEEAFSEGRVDAVVTFEPQRSRLLKAGA
                     REIFSSREIPGEILDVLIARRDAIEEHSDDLQNVVDGWFEVNRHIQHDASLLSRVANQ
                     LKLTPGETRQAYAGIHIPDRSENIRELSGPLEEHAARLGKIMAQKELLRKAPDTRGIA
                     DDSFVKRAPEK"
BASE COUNT          435 a          535 c          517 g          539 t
ORIGIN      
        1 cggagatgtt ctgctctttc tacgccctct gacagggtta gatctggaag tatgacaacg
       61 aattcttctc cgccatagcg tgctgtgaat tcgccgccac gtctgaaatg cgactgaaga
      121 atgttggaaa tagcaatcag gcaatcgtca ccggcctgat gcccgtagtg gtcattgaac
      181 attttgaaat gatccacatc gcagacaagg agtgttacag gttggccgtg tctttgtgca
      241 cgacggattt cctcctcaat tcgaacgtca agggatcttc tattgtagag gccagtaagg
      301 gaatcggtgg tggctgcctg tgcaagctga gtcaccgcat gctccagctc ctgattggct
      361 gcgatcagat cctgttgcat ctgtcgccgc tccgaaatgt cgcggataat tccaatgaac
      421 agcaactgac cttctgcgcg tagttcgctt actttcaagt caatgggaaa ttttgtgccg
      481 tcttttctgc atgcttccgc ttcgcgagcc atgccgatta cgtttttcac tcctgtagcc
      541 acatagtttg ccaggtatac atcatgcatg gaatgatgcg gttctggcat caagacgctg
      601 atattctggc ctgcaagttc tccgtccctg tatccaaaga tggcagatcc tgcggggttg
      661 atggattgaa tggttcctct ggtatctatg atgatgagac cgtcgagcaa ggaatcaaat
      721 acttcgcgaa gccggctttc tctatctttg agtgcaattt cagtttcgtt tactcgcgca
      781 aacaaatcgt ccacatcccg tccaagttct gcgatttcat ccgcgccttc gagtcgtaga
      841 cgaacgcttc tgtttccact tgcaaatttc ttgattccgg ttgaaatagt ttccgttctc
      901 cgagtgactc gcatcgtgag cacgagccac aatgcaaacg cacctgcaag caggccaatg
      961 gcagtgacag tcatctgcgt ccgtagttcg cgatttgttt cgcggattcg gtacgcaagg
     1021 tccagctcgt agtacacaaa cccgcatcgc cctccgcgca gcccgtttct gcctggagcg
     1081 cacatgcctg cataaccgac tacaaaatca gtccccgcgt ccgggaggat gcgcgggccc
     1141 gaagccagag ggtcttcaag aaacttcgcg tgaacacggg ggtagcgatt ggcgacgagc
     1201 tcgcctacgt cctgaatatt tgtggaagca atgatcttcc catccacatc ggatgcgaag
     1261 aggacagaaa gatctgtttc tgaggcaaag ctgatcatgg cggctcgcaa gggctcaatg
     1321 ttcgtggtcc gaaaatagac ttcccccgct gattcgagga acgccagcct ttctgcaatc
     1381 ttgattctac tttcacgcgt gagtctgccc atgcgcactg tgcgcgcagc agcgtacgct
     1441 acgaagagtg agctcaaggc aaacgaaacg gcaagtgcaa ggatcaagaa acgaaccgtg
     1501 cgaaatcgtt tcatttctct ggcgctctct ttacgaagct gtcgtctgcg attcctcgcg
     1561 tgtcaggagc ttttctgagc aattctttct gggccatgat cttgcccagt cgcgctgcgt
     1621 gttcttcaag tggtccggaa agctcgcgaa tattttctga tctgtcaggg atgtgaatgc
     1681 cggcgtaggc ctgtctggtt tccccgggtg tgagcttaag ttgattggcc acgcgagaca
     1741 gaagggacgc atcgtgctgt atatgtctgt ttacttcgaa ccatccatcc actacgtttt
     1801 gtaaatcgtc gctatgctcc tcgattgcgt ccctgcgcgc aatgagtacg tccagaatct
     1861 cgccgggtat ctcgcggctt gaaaatattt cgcgagctcc cgctttcaag aggcgggacc
     1921 tctgtggttc aaatgtgaca actgcgtcta ctcgtccctc actgaaggct tcctcgtgtt
     1981 cattgatcct gattgggaca attgtgatgt ccgcgctcgt catttt
//
LOCUS       DAORFF010000647         1991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_46158_length_1991_cov_3.389463, whole genome shotgun sequence.
ACCESSION   DAORFF010000647 DAORFF010000000
VERSION     DAORFF010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46158_length_1991_cov_3.389463"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PKK76_18640"
     CDS             complement(<1..707)
                     /locus_tag="PKK76_18640"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNL70552.1"
                     /translation="MGKTALITGITGQDGSYLAEFLLSKGYKVFGMVRRSSSENFDRI
                     EHIRDKVILRQGDLLDQLSLLNLIEESAPDEIYNLAAQSFVPTSWTKPLLTGEFTALG
                     VTRMLEAMRAVKSKARFYQASSSEMFGMVQEVPQKESTPFYPRSPYGVAKVYGHFITV
                     NYRESYNMFAVSGILFNHESPRRGKEFVTRKVTDGVARIKLGLQTELKLGNLDAKRDW
                     GFAGDYVRAMWLMLQQD"
     gene            822..1337
                     /locus_tag="PKK76_18645"
     CDS             822..1337
                     /locus_tag="PKK76_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70553.1"
                     /translation="MLQGKGVEKMGIEARIAERAESRHYDDADVKRVDSLSLSPLDSG
                     KWGFDAAATSELFQKPVPAPDFRSWKFRFIRGPLKPLARKIYVTLAQMFDRMSENKTQ
                     AFYDVVHELADLRRENAQLKERIQALEAKSRQDSRDPAVPSAENPRPPRPVERGAPGI
                     SSRSGNRPRPL"
     gene            1344..>1991
                     /locus_tag="PKK76_18650"
     CDS             1344..>1991
                     /locus_tag="PKK76_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNL70554.1"
                     /translation="MDEQVLHSLQWIESMPSWVMFAFFFFSAFMENVFPPYPGDTILA
                     FSGFLAARGVMSLPIVFFAGFAGSVAGAFVMYMLGHRALELARRMERSVSRPAWLKNS
                     LHEAVSQESIEKTGLWFNRYGLYFVIVSRFSAGVRFFVSIVAGISRVSLPLYMLCFAL
                     GVVLWNGLLTAGGYTLGRNWELVLVYLKLYNTVFIIVAVVAVAGFVFWKWRSSNIH"
BASE COUNT          391 a          493 c          551 g          556 t
ORIGIN      
        1 ttatcctgct ggagcatgag ccacatggcg cgcacgtaat ctccggcaaa tccccagtcg
       61 cgtttcgcgt ccaggtttcc aagcttgagt tccgtttgaa ggccgagttt gatacgagct
      121 actccgtctg tcaccttacg cgtaacaaat tcctttccgc ggcgagggga ttcgtgattg
      181 aacagaatgc ctgaaaccgc gaacatattg taggattcgc ggtagttcac agtgataaaa
      241 tgcccgtaca cttttgcaac gccgtaaggg ctgcgaggat agaaaggtgt gctttctttt
      301 tgagggactt cctgcaccat tccaaacatt tccgagctcg atgcctggta gaatcttgct
      361 tttgatttta ctgctctcat tgcttcgagc atacgagtca caccgagtgc agtgaattct
      421 cccgtcagga gtggcttcgt ccaggaggtc ggcacaaagg attgcgctgc caggttgtag
      481 atttcatccg gggcggattc ttctattaaa ttgagaaggc tgagctggtc gagcaggtct
      541 ccctggcgga ggatcacttt gtcgcgtatg tgctcgattc tgtcgaagtt ttcgctcgat
      601 gagcggcgaa ccatgccaaa aaccttgtag cctttcgaaa gcaagaattc tgcgaggtag
      661 gaaccgtcct ggcccgtgat tcccgtgatc aatgccgttt tacccacgat tgcgctcctt
      721 gggccagatt cgccaaattc gctattttta ccattccttt tgcttgttgt cctggaagca
      781 aaaatgagtg cggtccccgg cctgcgagca atggtggcct catgcttcag ggcaaaggcg
      841 tggaaaaaat ggggattgag gctcgaattg cggagagagc tgaatctcgc cattatgacg
      901 atgctgatgt caagcgtgtg gattcccttt ctctatctcc gctcgattct gggaagtggg
      961 gttttgatgc cgcggcaacg tcagagcttt ttcaaaagcc agtccccgct ccggattttc
     1021 gtagctggaa gttccgcttc attcgcggtc ctctcaagcc acttgcccgc aagatctacg
     1081 ttactctcgc acagatgttc gatagaatgt ctgaaaataa gacccaggca ttttacgatg
     1141 ttgtgcacga gcttgcggac cttcggcggg agaacgctca gctgaaggag cgcattcagg
     1201 cactcgaagc aaagtcgcgg caagactcgc gagatcctgc tgttccttca gcggagaatc
     1261 ctcgcccgcc acggccggtc gagcgcggtg cgcctgggat ttcttcaagg agtgggaatc
     1321 gtccgcggcc tctctagttg tgcatggatg agcaggttct tcattctctt cagtggatcg
     1381 aaagcatgcc ttcctgggtc atgtttgcat tcttcttttt ttctgcgttc atggagaacg
     1441 tttttcctcc gtatcccgga gacacaattc tagcattctc gggctttctt gccgcccgcg
     1501 gagtgatgtc cttgcccatt gtattctttg ctggattcgc gggcagtgtg gccggcgcat
     1561 ttgtgatgta tatgcttgga cacagggctc tcgaacttgc gcggagaatg gaacgcagcg
     1621 tttcgcgtcc ggcctggttg aagaacagtc tgcatgaagc ggtttcgcaa gaaagcatcg
     1681 agaagacggg tctgtggttc aatcgctatg gtctttactt cgtcatcgtg tcgcgatttt
     1741 ctgcgggagt ccgttttttt gtgagcatag tggcgggaat ttcgagggtc tctctgcccc
     1801 tctacatgct ttgcttcgcc ctgggtgttg tgctgtggaa cgggcttttg acggcgggag
     1861 gctacactct cggccgcaac tgggaactcg tacttgtata tttgaagttg tacaatacgg
     1921 tatttatcat cgttgctgtg gttgcggtcg caggcttcgt attctggaag tggcggtctt
     1981 cgaatattca t
//
LOCUS       DAORFF010000648         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_46194_length_1990_cov_4.063566, whole genome shotgun sequence.
ACCESSION   DAORFF010000648 DAORFF010000000
VERSION     DAORFF010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46194_length_1990_cov_4.063566"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..728)
                     /locus_tag="PKK76_18655"
     CDS             complement(87..728)
                     /locus_tag="PKK76_18655"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HNL70555.1"
                     /translation="MKVEIWSDVMCPFCYIGKRKFERALAQFPERESIEIEWKSFQLN
                     PGMKTEPGKSINEYLAEVKGWSVEEAKKMNDRVSQIASAVGITFNFDQAIVANSFDAH
                     RLSHMAKKHGLQDQMEERLFAAYFTEGKNTADHDTLVALGAEVGLDAAEVASMLKSQD
                     YANDVRFDVHEATQFGIRGVPFFVLARKFGVSGAQEPEAFLEALTTAVAQTKV"
     gene            851..1207
                     /locus_tag="PKK76_18660"
     CDS             851..1207
                     /locus_tag="PKK76_18660"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNL70556.1"
                     /translation="MTYGDLDYSLREVGRVHVLDLRGELTLHNANELKELISSMVEQK
                     KLFVLINLENVQGIDSSGLSAFVAGQTYLTRQKGQLKLASARPAIRKLLKATLLDQLL
                     AVFDSVDEAMRSFPAR"
     gene            complement(1147..>1990)
                     /locus_tag="PKK76_18665"
     CDS             complement(1147..>1990)
                     /locus_tag="PKK76_18665"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNL70557.1"
                     /translation="IAPPVMAAFLRFFMAGILFLILAHNQGAFREVRGTLWLNILVLA
                     FVGIFLYNLFFFYGISLTSTTNGSLIAGANPAVTALLSFVFRGERLAGRRWLGIFISF
                     LGVVLVISQGSLEVLLHMKMNPGDLLIVCAATCWAIYSILGRDVMRQISPLAASAFAC
                     YLGSIMLFPAALATTDQVSWRSDWMFWLEVAYMAIFASVLAYKWWYEGVLEIGASRSA
                     VYVNLVPVFVMLLNVFMGHFPSEAQLGGAALVLVGVYLTSGNIQRAGKERMASSTESN
                     TASN"
BASE COUNT          548 a          542 c          461 g          439 t
ORIGIN      
        1 cacaatctca cttgtggcaa taagcatgat ccccgaaagg attcgctact caggcccaca
       61 attgaaaagt ttgtgagtca ttcggatcaa actttcgtct gcgcaacagc ggttgtcagg
      121 gcctcgagga atgcctccgg ttcctgagcg ccagaaacgc caaacttcct tgccagtaca
      181 aagaacggaa ctcctcgaat tccaaattgc gtcgcctcat gaacatcgaa gcgcacatca
      241 ttcgcgtaat cctgactctt gagcatagag gccacttccg ccgcatccag tccaacttcg
      301 gccccgagcg caacgagtgt gtcatggtcc gccgtattct ttccctcagt gaaatacgcg
      361 gcaaacaaac gctcttccat ctgatcctgc aggccatgct tctttgccat gtgagacagt
      421 ctgtgcgcat caaacgaatt ggcaacaatc gcctggtcaa aattgaaagt tatgccgaca
      481 gcggaggcaa tctggctaac gcggtcgttc attttctttg cttcttccac gctccaacct
      541 ttcacttccg ccaggtattc attgatgctc ttcccgggct cggtcttcat gcctggattc
      601 agttgaaaac tcttccactc tatctcaatg gattcgcgtt cggggaattg agccagggcc
      661 ctttcgaatt tccgtttgcc tatatagcaa aacggacaca tcacatcgct ccagatttca
      721 actttcatgt tcacctttga cgaagagtct cttgacgcgt acgactccct cgcacgatcc
      781 tgggatcagt gaaagaaagt gcggacgtag caaccacctt atttcgcgca gaggtttcgc
      841 ggaaattgcc atgacatacg gggatcttga ctattcgctg cgggaagttg gccgggtcca
      901 tgtcctggat ctgcgtggcg aattgaccct gcacaatgcg aatgagctaa aggagctgat
      961 cagtagcatg gtcgaacaaa agaagctatt cgtattgatt aacctggaaa atgtccaggg
     1021 gattgattct tcaggactca gcgctttcgt tgccggacag acgtatctta cgcgccagaa
     1081 aggccagctc aaactggcct cagccaggcc agcgattcgc aagctactga aggccacgct
     1141 actcgatcaa ttgctcgcag tgtttgattc tgtggacgaa gccatgcgtt cttttcccgc
     1201 gcgctgaata ttcccgctgg tcaaatacac cccaaccagg accagagccg cgccgccgag
     1261 ctgagcttcg ctcgggaaat gccccataaa aacattcaga agcatcacaa agacaggcac
     1321 cagatttaca tagactgcag aacgcgaagc ccctatctcg agtacacctt cataccacca
     1381 cttataagca agaacggaag caaagatcgc catgtaggcc acttccagcc agaacatcca
     1441 atcgctgcgc cagctcactt gatctgttgt ggcaagcgcc gccgggaaaa gcatgatgga
     1501 tccaagatag caagcaaagg cggacgcagc aagtggcgag atttgccgca taacgtcccg
     1561 accaagaatt gaatagatgg cccagcatgt agcggcacaa acaatcagca aatcacctgg
     1621 attcattttc atgtgaagaa gaacttcaag cgatccctgc gaaatgacca gaacaactcc
     1681 caaaaaggaa atgaaaatac caagccacct tctgcctgct aaacgctcac ctcgaaatac
     1741 aaaactgaga agagcagtca ccgctggatt tgcaccggcg atcaaggatc cattcgttgt
     1801 agatgtaaga ctgattccat aaaagaaaaa cagattgtag agaaagattc caacaaaggc
     1861 gagaacgaga atgttcagcc acagagttcc tctcacttcg cgaaacgctc cttgattgtg
     1921 cgcaaggatg aggaagagga ttccggccat aaagaatcgt aagaatgcag ccatcacagg
     1981 aggcgcgatt
//
LOCUS       DAORFF010000649         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_46195_length_1990_cov_3.957106, whole genome shotgun sequence.
ACCESSION   DAORFF010000649 DAORFF010000000
VERSION     DAORFF010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46195_length_1990_cov_3.957106"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKK76_18670"
     CDS             <1..454
                     /locus_tag="PKK76_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNL70558.1"
                     /translation="APGKHLQVIDLAGKILVLGVSDAGVQLVTAVDDARTADRIRIWH
                     QSKPTLPKPENLIQKWLLGFGRADLKFWSRPEPPPPDFRTQLGQAVGPSFTAAEPAES
                     VERVRPGASEILEQDLFGPEDELRRMLREQKNRLEKIREPGKKKPSTG"
     gene            548..1324
                     /gene="fliP"
                     /locus_tag="PKK76_18675"
     CDS             548..1324
                     /gene="fliP"
                     /locus_tag="PKK76_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772588.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNL70559.1"
                     /translation="MSRAKLLTILALLFIAAGSLSAQVPGFQIPVIDGVRGARDGREA
                     SFSLLLIVLFTLLSLAPALVMMMTSFTKVVIVFDFVRRALSLQNMPPNQVLFGLALFV
                     TFFIMAPTVQEFNKSALQPYNRGELSTGKFMDEGMKPFRTFMIKQIGKDGAKEIGLFI
                     GLSGKDKNEIKTVDDIDSYILIPAFMLSEIKKAFIIGIVIFIPFIVIDLIVSSTLMSM
                     GMVMLPPAMISLPFKIILFILVDGWHLITYNLVISYNGGG"
     gene            1324..1587
                     /gene="fliQ"
                     /locus_tag="PKK76_18680"
     CDS             1324..1587
                     /gene="fliQ"
                     /locus_tag="PKK76_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNL70560.1"
                     /translation="MLEADAIKLIQDALVVTLKVSAPMLILSLFVGLVVSILQTTTSI
                     QEQTLTFVPKLLAVFAALSLFASYIIQTLVDYTKDVFGLIQRF"
     gene            1541..>1990
                     /locus_tag="PKK76_18685"
     CDS             1541..>1990
                     /locus_tag="PKK76_18685"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNL70561.1"
                     /translation="MWTIRRTFSASSRDSKGIFLERFDHNTQYFLLIFARMVGMVFVA
                     PVFSADSIPYRTRMMIAFLLSAVLYPVSVNYLPPLPSGPVSFIGEATAQAALGIIIGF
                     LIQMIFAAFQIAGELFSVQSGLSFAEVLDPASQTTMPIIGTLKSSIGL"
BASE COUNT          474 a          520 c          455 g          541 t
ORIGIN      
        1 ggcccccgga aagcatttac aggtcattga tcttgcagga aaaatcctgg tactgggcgt
       61 gtctgatgcg ggagttcaac tggtcactgc agtggacgac gcacgcacag ccgatcggat
      121 tcgaatctgg catcaaagca agcctactct accaaagccc gaaaatctga tccagaaatg
      181 gctcctgggg tttggccgcg ccgatttgaa attctggtcc aggcctgaac ctcctcctcc
      241 tgactttcgc acgcagcttg gccaggcagt gggcccatcg tttactgcgg ctgaacctgc
      301 agaatcagta gagagagtga gacccggtgc atcggagatc ctggaacagg acttgttcgg
      361 gccggaagac gagctcagaa gaatgctacg cgaacagaaa aatcgcctgg aaaagatccg
      421 tgaaccgggc aaaaagaagc catctacggg ctgaaaaagg cccgcttttg ccaaaaaaat
      481 acttgtactt ctaaaatatt cttgtactca tctcagagcg gaggaacctg ggccctgggg
      541 ccgtattatg tcacgagcta aactgcttac gattctggct ctgcttttca ttgcagcagg
      601 gtctttgtct gcccaggtcc ccggattcca gatcccagtg atcgatggag tccggggagc
      661 gagggatggg cgagaagcat ctttctccct gctcctgatc gtactgttca ctcttctcag
      721 cctcgccccc gctcttgtaa tgatgatgac ttctttcacg aaggttgtca ttgtatttga
      781 ttttgttcgc cgcgcgctct ctctccagaa tatgccgccc aaccaggtgc tctttggcct
      841 ggctttgttt gtaacgttct tcatcatggc gcccaccgtg caggaattca acaagtctgc
      901 gcttcaacca tacaatcgcg gagaactatc taccggtaag ttcatggacg aaggaatgaa
      961 accctttcgc acattcatga tcaagcaaat tggaaaagac ggcgcaaaag agatcggact
     1021 gttcattggc ctgtcaggca aagacaaaaa cgaaatcaaa accgtagacg acattgattc
     1081 ctacattctg attccggctt tcatgctctc tgagatcaag aaggcattca tcattggcat
     1141 cgtaattttc attccattca tagtaattga tctgattgtt tcatccaccc tcatgtcgat
     1201 gggcatggtg atgctcccgc ctgccatgat ttctctgcct ttcaaaatca ttttgttcat
     1261 cctcgtggac ggatggcatc tgattacata caatcttgta atttcgtata acggcggtgg
     1321 atgatgctcg aagcggacgc aattaaactc attcaagacg cacttgttgt aacgctaaag
     1381 gtatcagcgc ccatgctgat tctttctctg ttcgttggac tcgtagtatc cattcttcag
     1441 acaaccacat ccattcagga acaaactctt acgtttgttc ccaagctgct tgctgtgttt
     1501 gccgcgctct cccttttcgc aagctacatc attcagaccc ttgtggacta tacgaaggac
     1561 gttttcggcc tcatccagag attctaaggg tatttttctg gaacgatttg atcacaacac
     1621 tcaatacttc ctgctcatct tcgcaagaat ggttggaatg gtgtttgttg ctcccgtatt
     1681 cagcgcagac agcattccat atcgaacacg catgatgatt gctttcttgc tttctgcagt
     1741 gctgtatcct gtttcagtca actatttgcc tccgcttcct tccgggcccg tctcattcat
     1801 cggagaagcg acagcacagg cagcactggg catcatcatc ggatttctaa ttcagatgat
     1861 ctttgccgca ttccagattg caggtgaatt attttccgta caatctggat tgtctttcgc
     1921 agaagtcctg gatccggcat cgcagaccac catgccaatc attggcactc tcaaaagcag
     1981 catcggcctt
//
LOCUS       DAORFF010000650         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_46253_length_1989_cov_3.651499, whole genome shotgun sequence.
ACCESSION   DAORFF010000650 DAORFF010000000
VERSION     DAORFF010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46253_length_1989_cov_3.651499"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PKK76_18690"
     CDS             complement(<1..778)
                     /locus_tag="PKK76_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004760800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HNL70562.1"
                     /translation="MPKTSKDVIEFAKKNNILFYDFRFTDIKGIWHHVSYHVDSVSED
                     ILKGVPFDGSSIAAWQPINQSDMLLIPDLDSLFIDPFTADPTLVVFCDVYDIYKKQPY
                     EKDPRSIAKKALKYLAESGIGDAAYFGPENEFFIFDDLKIKDEINAQSYEIDSTEGVW
                     RSNSDSEGSINTGHRPYTKGGYFPVAPVDSQVDIRAEIVKTLHKIGMETFVVHHEVAQ
                     GQGEIGVKFGTLIEAADNVQKLKYVVKMVAHRHGKTATFMP"
     gene            complement(1083..>1989)
                     /locus_tag="PKK76_18695"
     CDS             complement(1083..>1989)
                     /locus_tag="PKK76_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNL70563.1"
                     /translation="RKALRLLAYLPDNNHSFVPFHDTGDPIDRETIEEDILLRRTFAS
                     PSGFNTPFDITLLIQQIVDHGEYFELQPQRAGNLVTCFGRVGGHVVGFLANNSAVSSG
                     QIDINAAYKGARFIRFCNLYNIPVIFLEDTTGFLPGREQEAGGIVLAGRALLDSIIDL
                     RVPRLLFIVRNAFGGAYASWNSYHTGADMVFAFPGARVAVMGPAGREYIYKDELVKAR
                     AKLQEAIKAGREEEGKAEFNRVIAEMNARYENELMNPKEALALGSISRIVMPGDSRRV
                     LGQALNFYMRRYKPGPLSEPQREFH"
BASE COUNT          454 a          490 c          514 g          531 t
ORIGIN      
        1 tgggcataaa tgtagcggtc ttgccatgtc tgtgcgcaac catcttcact acgtatttca
       61 gcttctgaac attgtctgca gcttcaatca gggttccgaa ttttacaccg atctctccct
      121 gtccttgcgc aacttcgtgg tgaactacaa acgtttccat tccgatcttg tgcagggtct
      181 ttacgatttc ggcccggatg tccacctgtg agtccacagg ggccacagga aaatatccac
      241 ctttcgtgta gggacggtga cctgtgttga tgcttccttc gctgtcggag ttggatctcc
      301 agacaccttc cgtactatca atctcgtagg attgagcgtt gatctcgtct ttgattttga
      361 gatcatcaaa gatgaagaat tcgttctccg gtccaaaata cgctgcatcg ccgattcccg
      421 attctgcgag gtacttcaga gctttcttcg cgatgctgcg aggatctttt tcgtaaggct
      481 gcttcttgta gatgtcataa acgtcgcaga aaacgacgag cgttggatcg gctgtgaacg
      541 gatcgatgaa cagggaatcc aggtccggga tcagaagcat gtccgactgg ttgattggtt
      601 gccaggcggc aatggagctg ccgtcaaacg ggactccttt cagaatgtct tcgcttaccg
      661 aatccacgtg ataggacaca tggtgccaga ttcctttgat atctgtgaaa cgaaagtcgt
      721 agaagaggat gttattcttc ttcgcgaatt cgatcacgtc ttttgatgtt ttgggcatcg
      781 gatttctctc ttatgggtag aatacaaatg cctacgcaat aatcgcgccg ggcatgcgcg
      841 aaatgcacag aaagcacacg ttttctggca gaagcctgtt tgcgcgattt tggatcggcg
      901 tgccgggcag cgcggagtgg gagattgctg tacacgttta cgacgatttg cgtacaaaaa
      961 aggcgcacag tgcctttaaa atgggcaggt ctggttcgac ggcggcaaat gccagctctc
     1021 gcaaaatcgc gaggttggat tcggcgctct tgccctccaa ccggaagatt ccgccccgac
     1081 tctcagtgaa actcgcgctg tggttctgaa agtggacctg gcttgtagcg tctcatatag
     1141 aagttgagcg cctgtccaag tactctgcga gaatcaccgg gcattacaat gcgcgaaata
     1201 gatccgagtg ctagagcttc tttcggattc atcagctcat tttcatacct ggcattcatc
     1261 tctgcgatca ctcgattgaa ttctgctttg ccttcttctt ctctgcctgc tttgatcgct
     1321 tcctgcaatt tcgcacgggc tttgaccagc tcatctttgt aaatgtactc tcgtccagcg
     1381 ggccccatta cagccacgcg cgctccaggg aaagcgaaaa ccatgtctgc gcctgtgtgg
     1441 tatgaattcc acgacgcata cgctccacca aatgcatttc gcacaatgaa tagaagtctt
     1501 ggaacacgaa ggtcaatgat cgaatcgagc agggcacgtc cggccaggac aataccgcca
     1561 gcttcctgtt cgcgacctgg caagaatcca gttgtatctt ccaggaagat cactggaatg
     1621 ttgtagagat tgcagaatcg aatgaagcgt gctcccttgt aggctgcatt gatgtcaatc
     1681 tgaccgctcg atactgcaga gttgtttgca agaaagccaa ccacatgtcc gcccactcta
     1741 ccaaagcagg tgaccaggtt tccagcgcgc tgaggttgca gttcgaaata ctctccgtgg
     1801 tccacgatct gttgaatcaa gagagtgatg tcaaaaggag tgttgaatcc agacggggag
     1861 gcgaaggtac gtctgagaag gatgtcttct tctatggttt cgcggtcgat tggatctcct
     1921 gtgtcgtgga atggcacgaa gctgtgattg ttgtccggaa gatatgccag aagacggaga
     1981 gcttttctg
//
LOCUS       DAORFF010000651         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_46256_length_1989_cov_3.142192, whole genome shotgun sequence.
ACCESSION   DAORFF010000651 DAORFF010000000
VERSION     DAORFF010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46256_length_1989_cov_3.142192"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PKK76_18700"
     CDS             <1..244
                     /locus_tag="PKK76_18700"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNL70564.1"
                     /translation="RPGMLLTAAREMNLDLSRSFMVGDRWRDVDAGNAAGCHTIFVDY
                     GYTEELKSRPDYVIDAPQKMTDIILQQKEEKGESNS"
     gene            500..958
                     /locus_tag="PKK76_18705"
     CDS             500..958
                     /locus_tag="PKK76_18705"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HNL70565.1"
                     /translation="MSTFTSKIPVTNTKAEPSYDLIRRLTQKGARLNVTAVMTIAQTE
                     KIVEALKGTKGAVVSIFAGRVADTGRDPVPHMVEALKIVQKNPNTELLWASPRELLNI
                     FQADEIGCHIITVTNDILKKLEIVGKDLDEYSLDTVKMFYKDATSAGFSL"
     gene            974..1957
                     /locus_tag="PKK76_18710"
     CDS             974..1957
                     /locus_tag="PKK76_18710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNL70566.1"
                     /translation="MQKKYKLSVVVPVYNEEKNLPVFLERTLAVLKKLRCEYEIIFAM
                     DPSRDRTREMILEYRAKNPSIKLLEFSRRFGQPAATLAGIHYCTGDACIIIDADLQDP
                     PELMEAMVEKWQEGYHVAFGQRVSRTGETWVKILVSYVGYWVINRISQVAIPRNTGDF
                     RLISREVIEELRGLKEGHGFLRGLVGFVGFSQVAVPYSRDPRLAGQGNYNRFLGSLTI
                     GFNGIVGFSRYPLQFISLGGLFISFISFLTAIVYIALSLAGVHIPWGNPTLVILISSL
                     SGLQLLSIGIMGEYVGRIYDEVRDRPIYIVSGAHGFQPQSKPAGRKRGGRR"
BASE COUNT          512 a          460 c          537 g          480 t
ORIGIN      
        1 gcgtcccggt atgctactga cggcggcccg tgagatgaat ctggatctct cacgaagctt
       61 catggtgggc gatcgatggc gagatgtaga tgctggaaac gcggcaggct gccatacgat
      121 cttcgtggac tatggctaca ctgaagaact taaatccagg ccggactacg tgattgacgc
      181 acctcaaaaa atgacggaca ttattttaca gcagaaggaa gaaaaaggag aaagcaattc
      241 atgaagaaag tatcagatct tcacattaag attttcgcgg atggtgcaga aaaagcagga
      301 atgcttgaaa tgtacaagca gccgtttatc agcggattca caaccaatcc tacgctcatg
      361 aacaaagtgg gcatcaaaga ctatcgcgcg tttgcgcagg acatagtgaa agcgattccg
      421 gatcgtccaa tttcttttga agtttttgca gatgattttg cggagatgga gcgtcaggcg
      481 ggcgagattg ctacctgggg tgagtacgtt tacatcaaag attccagtaa ccaatacgaa
      541 agcagaacct tcctacgact tgatccgccg cctcactcag aagggagcac ggttgaatgt
      601 gactgcggtc atgacaatcg cgcaaacgga gaaaattgta gaagcactca aaggaacaaa
      661 gggtgcggtg gtttccatct tcgcaggacg cgtagcagac acaggccgcg acccggttcc
      721 acatatggtg gaagcattga aaatcgttca gaagaatcca aacacagagc tactctgggc
      781 ttcccctcgt gaactcttga atatctttca ggcggatgaa atcggatgtc atatcatcac
      841 cgtaaccaat gacattctca agaagctgga aattgtaggc aaagacctgg atgaatattc
      901 tctcgataca gtgaagatgt tctacaaaga cgctacaagc gcaggtttct ctctctagtc
      961 agcggaagac cggatgcaaa agaagtacaa actatcggtc gtcgttccag tttacaacga
     1021 agagaagaac cttcctgtat ttctggaaag aaccctggca gtattaaaaa agctgcgctg
     1081 cgaatatgaa atcatttttg cgatggatcc ctcccgggat cgcacccgcg aaatgattct
     1141 agaataccgc gcgaagaatc cttccattaa gctgcttgag ttctcaagaa gatttggtca
     1201 gcccgcggcc acgttagccg gcattcacta ttgtacggga gacgcctgca tcatcattga
     1261 tgcggacttg caggatccgc cggaactgat ggaggcaatg gttgagaaat ggcaggaagg
     1321 ctatcacgtt gctttcgggc agagggtttc gcgcaccggg gagacctggg tgaagattct
     1381 cgtgtcgtac gtgggctact gggtcatcaa tcgcatttcg caagtcgcaa ttcccagaaa
     1441 cactggtgac tttcgcctga tcagccgcga agtcattgag gagctccgcg gcctcaaaga
     1501 gggacatgga tttttgaggg ggctcgttgg ttttgtcggc ttttctcagg tggctgtgcc
     1561 atattcgcgg gatccgcgtc tggcagggca gggaaactac aatcgcttcc tgggatcact
     1621 gaccatcggt ttcaatggga tcgtaggttt ttccaggtat ccgttgcagt tcatttccct
     1681 cggcggattg tttatttctt ttattagttt tctgactgca atcgtctaca ttgcactgag
     1741 tcttgcgggc gtccatattc cgtggggaaa tcctactctg gtcattctga tttcttcgtt
     1801 gagcggcctt caacttctga gcattggcat catgggggaa tacgtgggcc gcatttacga
     1861 tgaagtgaga gacaggccaa tctacatcgt ctcaggggcg cacgggtttc aaccgcagtc
     1921 caagcccgcc ggccgcaaac gcgggggtcg gagatgaagg cattcatcac tggcggcgcg
     1981 gggttcatt
//
LOCUS       DAORFF010000652         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_46576_length_1982_cov_3.700571, whole genome shotgun sequence.
ACCESSION   DAORFF010000652 DAORFF010000000
VERSION     DAORFF010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46576_length_1982_cov_3.700571"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1796)
                     /locus_tag="PKK76_18715"
     CDS             complement(<1..1796)
                     /locus_tag="PKK76_18715"
                     /inference="COORDINATES: protein motif:HMM:NF039196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNL70567.1"
                     /translation="MLKNLKITHRLLILIVVFTIPLGVLAFLEISSLVEVIHFKRLRR
                     TAFVMMHPMIDARTKINFRRGIMALVARGEGSKDLLPDVDRFIDEQMEEENKLITQYG
                     DIFETKKAYVEARQAWEKLKQAGTGQSLESSYRSHTELSLALLAISRTYGSASTLDLE
                     EEGMLYYPMWISTSEVPRIYDNMFQLMSRTFDAVEDDKVDSFERASLRELVVNLEESR
                     DALKAELKTTDENYPGYLSRVSNKFQESEKRQDELANFVRRNVLEGDGKVDRKMMIAY
                     ASAAADKLEAFHDENGLALKEGYDTLERKKQYLLALLIALISTAMGVALFLGYLTTRN
                     LSRSLGYVIQNMDSIGSGKLDNQIDAQSKDEIGTILKYLASMQSNLRGTLEENLRVRT
                     AVDNSSTMVMIADTNRAIVYTNNAMEEFFRKAEKDIQKDIPSFDRNRVLGMKFDQFST
                     KGTSLTDLERSEVPLGGHILRVSTSAIVNVIGERLGTVLEWTDRTNEVHAEQEIESLV
                     SAAVRGDFAMRMDLQGKQGFFRKLGAEINNLMQVSASGLADVARVLGAMAEGNLTERI
                     TADYSGTFAELKDFSNNTATRLAEIIARVRESA"
     gene            complement(1798..>1982)
                     /locus_tag="PKK76_18720"
     CDS             complement(1798..>1982)
                     /locus_tag="PKK76_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70568.1"
                     /translation="VQSVIRPIGKMLGEVEGIGGSTILGDGAIALILDIPALAEGIVK
                     QDRVGRHMVDLRTVGA"
BASE COUNT          457 a          541 c          450 g          534 t
ORIGIN      
        1 gccgattcgc gaactcgagc aatgatctcc gccagccgcg tggctgtgtt gtttgagaaa
       61 tctttaagct ccgcaaacgt gccactgtaa tctgccgtta tgcgctctgt gagattgccc
      121 tctgccatgg caccgagaac ccgggccaca tccgcgagac cagaggcgct cacctgcatg
      181 agattgttga tctctgcacc cagtttgcgg aaaaatccct gcttgccctg caaatccatg
      241 cgcatggcga aatccccgcg cactgccgca gatacgagcg attcgatttc ctgctctgca
      301 tgaacttcgt tggttctgtc cgtccattcc agaaccgttc ccagacgctc gccgattacg
      361 tttacgatgg cacttgtgga tacgcgaaga atatgtccgc ccagaggcac ttctgatctt
      421 tccagatccg tgagactggt tccttttgtg ctaaactgat cgaacttcat tcctagaacc
      481 cggttgcgat caaaagacgg aatatccttc tgaatgtcct tctccgcttt gcggaagaac
      541 tcttccattg cgttgttcgt atatacgatc gctcgattgg tgtccgcgat catcaccatg
      601 gtagacgagt tgtctactgc agtgcgcact ctgaggtttt cttccagcgt gccccttaga
      661 tttgactgca tgctggccag gtattttaga atcgtgccga tctcatcttt gctctgggca
      721 tcaatctgat tgtccagttt gcccgaacca atggaatcca tattctggat cacatatcca
      781 agagagcgcg aaaggttcct tgtggttaag tagcccagga acaatgccac acccatcgca
      841 gtggagatca aagcgatgag aagagccagc aagtattgct ttttgcgctc cagagtatca
      901 tacccctctt tgagagccag gccgttttca tcatgaaagg cttctagctt gtcggcggca
      961 gcgctcgcgt atgcgatcat catcttgcga tctacctttc cgtctccttc gagtacattt
     1021 cttcttacaa agtttgccag ttcgtcctgg cgtttctctg attcctgaaa cttgtttgaa
     1081 acgcgcgata agtaccctgg atagttttcg tcagtagtct ttaattccgc tttgagagca
     1141 tcgcgtgact cttccagatt cactacgagt tcgcgaagag atgccctttc aaaggaatct
     1201 accttgtcat cttccacagc atcaaacgta cgagacatga gctggaacat gttgtcgtag
     1261 atgcgaggaa cctccgacgt agaaatccac atgggatagt agagcatgcc ctcttcttct
     1321 agatccagag tggacgcgga cccgtaagtt ctcgaaatag caaggagtgc gagcgaaagc
     1381 tctgtatggc tgcggtagga tgattccaga gattgacctg tgcctgcctg tttcagtttt
     1441 tcccaggcct gacgcgcttc tacatacgcc ttttttgtct cgaaaatatc tccatactga
     1501 gtgatgagtt tgttctcttc ttccatttgc tcgtctatga agcgatccac atccggaagc
     1561 aaatcctttg acccctctcc cctggcaacg agagccataa ttccgcggcg aaaatttatt
     1621 ttagtccgcg catcaatcat tggatgcatc attacgaaag cggttcggcg aagacgtttg
     1681 aagtgaatca cttccaccag actggaaatc tcgaggaacg cgagaacacc cagcggtatg
     1741 gtaaagacaa caattagaat cagaagcctg tgagtgattt taagattctt taacatttca
     1801 agctcccacc gttcgcaaat ccaccatatg acgtcccacg cgatcctgtt tcacaatccc
     1861 ttctgccaga gccgggatat cgaggatcag cgcaatcgcc ccgtcgccaa gaatggtgct
     1921 cccgccaatt ccttctactt cgcccagcat ctttccaatc ggacgaatca cggactgcac
     1981 tt
//
LOCUS       DAORFF010000653         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_46862_length_1976_cov_3.533576, whole genome shotgun sequence.
ACCESSION   DAORFF010000653 DAORFF010000000
VERSION     DAORFF010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46862_length_1976_cov_3.533576"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /gene="gatB"
                     /locus_tag="PKK76_18725"
     CDS             <1..1126
                     /gene="gatB"
                     /locus_tag="PKK76_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNL70569.1"
                     /translation="TFRKKGDESDTTIRIHRIHMEEDAGKLLHAVKNRSYVDLNRAGV
                     PLIEIVSEPDLRSSDDAYHYLQTLRNILRSAGVSECSMEQGSLRCDANVSVRPTEDAP
                     FGTRVEIKNLNSFKAVKAAIEYEMQRQEELIKDGGKVIQSTVLWDADNRVTRLMRTKE
                     DAEDYRYFPDPDLLPFRIDPAWVDGLKKTMPELPAAKRERYMKEYDLPEYDADVLTRD
                     LDVARYYEKVVSICKDGKKSSNWVKDEVLGITNKNDIPISAFACTPERLGRMIQLLNE
                     GKITGRIAKQVFEHVYEENLDPDKVIEKYGYKPVDVSSLPDIINKVLDANASVVTDIL
                     AGKDRARGFLVGQVMKETRGQAPAQEVNQLIDELVEKRRT"
     gene            complement(1127..>1976)
                     /locus_tag="PKK76_18730"
     CDS             complement(1127..>1976)
                     /locus_tag="PKK76_18730"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNL70570.1"
                     /translation="FLFVFAICGFFFTAWGALQGLSYWIVIPLIAFFTSILHEIEHDL
                     IHDLYYRGNRWIQNLMFAFIWILRPNTISPWKRKGIHLLHHKVSGSPPDIEERLITNG
                     MPYGFKRILCIMDGFLNMILRRREVERIPGMRTFNIRLASLPMVPIFYGSILVECLLI
                     ATGNMSSIRTTLDFLMVVWVIPNVIRQACLVFLSSTMHYYGGVDSVFRQTQVLNAWYF
                     LPMQLFCFNFGSTHGIHHFVVNQPFYLREMVKRSAHAAFRENGIHFNDLSTFRSANHY
                     EPIGGI"
BASE COUNT          590 a          481 c          499 g          406 t
ORIGIN      
        1 aacgtttcgc aagaaaggag acgagtccga taccacgatc cgcatccatc gaattcatat
       61 ggaagaagat gcaggaaaat tactccacgc tgtgaagaat cgttcctatg tagacctcaa
      121 ccgcgcgggt gtacctctca ttgaaattgt atctgagccg gacctgagga gctcggatga
      181 tgcctaccac tatctgcaga ctcttcgcaa cattcttcgc agtgccggtg tgtcggaatg
      241 cagtatggaa cagggaagcc tccgctgcga cgcgaacgtt tccgtccggc caacggaaga
      301 tgccccgttt ggaacgcgcg tagaaatcaa gaaccttaac tcatttaagg cagtgaaagc
      361 agccatcgaa tacgaaatgc agcgccagga ggaactgatc aaagacggcg gcaaggtcat
      421 ccagtccacg gttctctggg atgcagacaa tcgtgtaaca cgccttatgc gaaccaaaga
      481 agacgcagag gattatcgct atttcccaga cccggacctg cttccatttc gcattgatcc
      541 tgcctgggta gacggactaa aaaaaacaat gccagagcta ccggcggcca agcgcgaaag
      601 atacatgaaa gaatacgacc tgcccgaata cgacgcagac gttctcactc gcgacctgga
      661 cgttgcccgt tactacgaaa aagtagtctc tatctgcaaa gacggaaaga agtcttcgaa
      721 ctgggtcaaa gacgaagttc tgggaatcac aaacaagaat gacatcccaa tctcggcttt
      781 cgcatgcact ccggaacgcc tgggtcggat gatccagctt ctaaacgaag gcaagatcac
      841 aggacgaatc gccaagcaag tatttgaaca tgtctacgaa gagaacctgg atccagacaa
      901 agtcatcgaa aaatatggct acaagccagt ggacgtttct tctctgccgg atatcatcaa
      961 caaggtgctc gacgcaaatg caagcgtggt cacagatatt ctcgcgggca aagatcgcgc
     1021 acgcggtttc ctcgtaggtc aggtcatgaa ggaaacccgc ggccaggctc ctgcgcagga
     1081 agtcaatcag ctcatagacg agctggtaga gaaacgcaga acctgatcaa atgccgccta
     1141 tcggttcgta gtgattcgcg ctgcgaaagg tagatagatc attgaaatgg attccgtttt
     1201 cacggaaggc tgcatgcgcg gagcgtttca ccatctcgcg caaatagaaa ggctggttca
     1261 ccacaaagtg atgaatgccg tgagtggatc caaaattaaa acagaacaat tgcatgggta
     1321 gaaagtacca tgcattgagc acctgggtct ggcgaaatac agagtccacc cctccgtagt
     1381 agtgcatcgt ggaagaaaga aaaacaagac aggcctggcg aatcacgttt ggaatgaccc
     1441 agactaccat caagaagtca agagtcgttc gaatagatga catgttcccg gtcgcaatca
     1501 ggaggcattc tacgagtata gatccgtaaa aaatcggcac catggggagg ctggccaggc
     1561 gaatattgaa tgtcctcatt ccagggatgc gctccacttc ccgcctgcga agaatcatat
     1621 tgagaaaccc gtccatgatg cacagaatgc gtttgaaacc ataaggcatt ccgtttgtga
     1681 tgagtctctc ttctatatcc ggtggacttc cagaaacctt gtgatgcaga agatgaatgc
     1741 ccttcctctt ccacggagag attgtgtttg ggcgaaggat ccagatgaaa gcgaacatca
     1801 gattttggat ccagcgattt cctctataat aaagatcgtg gatcagatcg tgctcgattt
     1861 catgaaggat agaagtaaag aaagcaatga gcggaatgac gatccagtaa gacagacctt
     1921 gcagggcccc ccatgcggtg aaaaagaatc cgcagatcgc aaacacaaac agaaat
//
LOCUS       DAORFF010000654         1974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_46942_length_1974_cov_3.374674, whole genome shotgun sequence.
ACCESSION   DAORFF010000654 DAORFF010000000
VERSION     DAORFF010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46942_length_1974_cov_3.374674"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /gene="purL"
                     /locus_tag="PKK76_18735"
     CDS             complement(<1..1348)
                     /gene="purL"
                     /locus_tag="PKK76_18735"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNL70571.1"
                     /translation="MTELFTEKEITVQDALEHGLTADEFERIRGFLGRLPTVTELGIY
                     SGLWSEHCSYKNSILQLKTLPTESPRLAAKTGEENAGALDIGDGLAVVFKIESHNHPT
                     AVEPYQGAATGVGGIMRDIFTMGARPICSLNSLRFGYPSEKRSRYLLSRAVKGIGDYG
                     NCLGVPVSGGEIFFDESFTRNCLVNAMTVGIVKKDKMVKAKAEGEGNPVFYVGARTGR
                     DGIHGASFASRDLSEESESKRSAVQVGDPFMEKLLMEASLELAQTDSFIGMQDMGAAG
                     LSCATSEMSAKGAVGMDIDLDLVPLREKGMNAYEIMLSESQERMLVVGKRGQENTIIR
                     IFNKWGLEAVEIGRVTTDGRLKIRWQGKLCTDIPADSLVLGGGAPRYKRDTSRPAYLD
                     QVADANQLLQKRPPQKEGMLALLSSPNVCSRRPVIEQYDSEVGLARVLGPGGDGGIA"
     gene            complement(1341..>1974)
                     /locus_tag="PKK76_18740"
     CDS             complement(1341..>1974)
                     /locus_tag="PKK76_18740"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HNL70572.1"
                     /translation="GAWIDSWPVIISFIAQTAFLPLLRPRLSLLVLVPIFTLHLLSSQ
                     PFFHTLSAYYMYTFTPFLLYGAFEGAKTFKHFRSAAIAGFCAAAAFTASTRKEVPMVV
                     LPDPSAGPEIRALLSKMEPGRKLWTHAFLSAQAPVQNPVFPLLHPTAPDYILIDPDRS
                     SPQGESPQDVEKALQIARRGCIVGRASGTVLYRVSQELTQETKEKPCTRD"
BASE COUNT          445 a          513 c          520 g          496 t
ORIGIN      
        1 gcgcgattcc accgtccccg cctggaccga ggactctggc cagacccact tcgctgtcat
       61 actgctcgat gactggtctg cgtgagcaga cgttaggcga cgatagaagg gcaagcattc
      121 cttctttttg cggaggtctt ttctgtaaga gctgatttgc atctgccacc tgatccaggt
      181 aggcaggtct gcttgtgtct cttttgtagc gaggtgcacc gccacccaga accagactgt
      241 ccgccggaat gtctgtgcac aattttccct gccagcgaat cttaagcctg ccgtctgtag
      301 tgacgcgccc gatttctacc gcctcaagac cccatttgtt gaagatacgt ataatcgtgt
      361 tttcctggcc gcgtttgccc acaacgagca tgcgttcctg ggattctgac agcatgattt
      421 cgtacgcgtt cattcccttt tcgcggagag ggacaagatc cagatcgatg tccattccaa
      481 cagcaccttt cgcactcatc tctgatgtgg cgcaggaaag tccggccgcg cccatatcct
      541 gcattccaat gaaggaatca gtctgcgcaa gctcgagaga cgcctccata agaagcttct
      601 ccatgaatgg atctcccacc tggactgcgc tgcgtttgct ttccgattct tctgaaaggt
      661 cgcggcttgc gaaactggcc ccgtggattc catctctgcc tgtgcgtgcg cccacataga
      721 aaacagggtt gccttctccc tctgcttttg cctttaccat tttgtctttc ttaacaatac
      781 caacggtcat cgcgtttacg agacagttac gtgtgaaaga ctcatcaaag aagatttctc
      841 cgcctgatac cggaactcca aggcagtttc catagtctcc gattcctttc actgctctac
      901 tgagcagata acggctgcgt ttttctgacg gataaccgaa gcgaagagag ttgagggaac
      961 agatgggtct ggcacccata gtgaagatat cgcgcatgat tccaccaaca cctgtggccg
     1021 cgccctggta aggctccact gcagttgggt gattgtgact ttcgattttg aaaacgactg
     1081 caagtccatc tccgatgtcc agtgcgcctg cgttctcttc tccggttttt gccgcaagcc
     1141 tgggcgattc ggtcggcaga gtcttcagtt gtagtatgga attcttatag ctgcaatgtt
     1201 ctgaccagag tcctgagtag attccaagtt cagttactgt gggcagcctt ccgaggaagc
     1261 cgcgaattct ttcgaattca tctgcagtca gcccgtgttc caatgcatcc tggacggtga
     1321 tttctttttc tgtgaataat tcagtcacgg gtgcatggtt tttcttttgt ctcctgtgtc
     1381 aattcctgag acacacggta gagaacggtt ccggaggctc gccccacgat gcatcctctg
     1441 cgagcaatct ggagagcctt ctccacatct tgcggtgatt cgccttgcgg cgagctgcga
     1501 tctggatcaa tcaaaatgta atccggggcc gtcgggtgaa gaaggggaaa aactggattt
     1561 tgcacgggcg cctgtgcaga gagaaaggca tgcgtccata gctttcgccc tggttccatt
     1621 tttgaaagaa gtgcgcgaat ttcagggcct gcggaggggt ccggcaggac caccatgggc
     1681 acttcctttc gcgtggaagc agtgaaggct gcagccgcac aaaagccagc gatcgcagca
     1741 gagcgaaaat gtttgaaagt tttagcgcct tcgaatgctc catacagtag aaacggcgta
     1801 aacgtataca tatagtacgc gctgagggtg tgaaagaatg gttgcgagct gagaagatgg
     1861 agagtgaaaa taggaaccag aacgaggaga gagagacgag ggcgcagaag aggcaagaaa
     1921 gctgtttgcg caataaaaga aataatcact ggccaggagt cgatccaggc tcct
//
LOCUS       DAORFF010000655         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_47145_length_1970_cov_2.733681, whole genome shotgun sequence.
ACCESSION   DAORFF010000655 DAORFF010000000
VERSION     DAORFF010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47145_length_1970_cov_2.733681"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKK76_18745"
     CDS             complement(<1..219)
                     /locus_tag="PKK76_18745"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNL70573.1"
                     /translation="MWIIGDIHGCLRELDALLDRIPEHEPLLFLGDYVDRGPDSAGVI
                     ERCLREKERSTFLMGNHESMMIHYFTDPG"
     gene            328..1545
                     /gene="sucC"
                     /locus_tag="PKK76_18750"
     CDS             328..1545
                     /gene="sucC"
                     /locus_tag="PKK76_18750"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNL70574.1"
                     /translation="MKIHEYQAKDILRRYGVTVPEGRVTGNPAEIGKLYDELGGGIVA
                     VKSQIHAGGRGKGIVYDKEDPATATKIQDGGVKVAKSREEAEKYGSNIVGNYLVTQQT
                     GGVGKRVLKVYLEKGTEIAREFYLSILVDRAVSKPIVIYSTEGGMDIEEVAAHTPDKI
                     HRLEVEPAIGLQAWQAREILYKLGVPAEAMKQGVDFLLKLWKACDDEDASMLEVNPLV
                     LTKQNTIIALDCKFTYDDNALYRHPEAVHLRDITEEDPLELRASEFNLNYVRLDGNVG
                     CMVNGAGLAMATMDIIKLAGEFPANFLDVGGGANAQTVANGFRIILGDPNVKAIFINV
                     FGGIVQCDRVANGIIQAAKDVNIHVPLIVRLKGTNADIAKDILAKSGLKILVASELKD
                     GADAVATAIRGGK"
     gene            1556..>1970
                     /locus_tag="PKK76_18755"
     CDS             1556..>1970
                     /locus_tag="PKK76_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002721166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNL70575.1"
                     /translation="MAVLVDKNTRVVVQGITGKEGSFHTSQMIEYGTRVIGGVTPGKG
                     GQKSEHGVPIFNTLQDAVDKEGANATVIFVPPPFAADAILEAVGAQIPLCICITEGIP
                     VRDMSRVYSVLKTSKTRLIGPNCPGVISPGKCKIGI"
BASE COUNT          552 a          467 c          522 g          429 t
ORIGIN      
        1 gcccggatct gtgaaatagt gaatcatcat cgattcgtgg ttccccatca ggaaggtgga
       61 tcgctctttt tcgcgtaagc atctttcgat taccccggca gagtcgggtc cgcgatccac
      121 ataatcgcct aaaaacagca gtggttcgtg ctccggaatg cggtcgagca gagcatccag
      181 ttctctcaag caaccgtgta tgtctccgat gatccacata gcgaaataag cacgatggcg
      241 gattgaaaaa gaattttcaa cacaccgtgt ccgcacaaat tagcacctgc ttctgcagag
      301 gaaacaaaaa cagcacagga ctgaaccatg aaaattcatg aataccaggc aaaagacata
      361 ctgcgacgct atggcgtgac agttccagaa ggaagagtca cgggcaatcc cgcggaaatc
      421 ggcaagttgt atgatgagct tggcggcggc attgtagccg taaaatcaca gatacacgca
      481 ggcggacgcg gaaaaggcat agtatacgac aaagaagatc ccgcaacagc gaccaaaatt
      541 caggacggcg gcgtaaaggt cgcaaagtct cgcgaagaag ctgaaaaata cggctccaat
      601 attgtgggca attatctagt aacgcaacag accggcggag tcgggaaacg agtgcttaaa
      661 gtatacctcg agaagggcac tgagatcgcg cgcgaattct acctttccat tcttgtagat
      721 cgcgcagtct ccaagccaat cgtgatctat tctacagaag gtggaatgga cattgaggaa
      781 gtggcggctc acactccaga caagatccat cgtcttgagg tggaaccggc gatcgggctg
      841 caggcctggc aggcgcgcga aatcttatac aagctcggag tccccgctga agcaatgaag
      901 cagggcgtgg acttcctctt gaaattgtgg aaagcgtgcg acgacgaaga tgcatccatg
      961 ctggaagtga atcctctggt tctcacaaag caaaacacaa tcatcgctct ggattgcaaa
     1021 ttcacgtatg acgacaatgc gctttaccgt catcctgaag cagttcatct ccgcgacatc
     1081 acggaagagg atccactgga acttcgcgca tctgaattca atttgaacta cgttcgcctc
     1141 gatggtaacg taggatgtat ggtgaacggt gccggtcttg ccatggcaac catggatatc
     1201 atcaaactgg caggtgaatt cccggcaaac ttcctggacg tcggtggtgg agcaaatgca
     1261 cagactgtgg caaacggatt tcgcatcatt ctgggcgatc caaatgtaaa agcaatcttc
     1321 atcaacgttt ttggtgggat cgttcaatgc gaccgtgttg ccaatggcat cattcaggca
     1381 gcgaaggatg taaacattca tgttcccctc atcgtgagat tgaaaggaac caacgctgat
     1441 attgcgaaag acattcttgc gaaatcgggt ctgaagatcc tggttgcaag tgaactcaaa
     1501 gacggcgcgg acgcagtggc tactgctatt cgcggaggaa aataaggaac ggtttatggc
     1561 agtactagtt gataagaaca ccagagtagt ggtccagggg atcacaggta aagaaggatc
     1621 gtttcatacg tcccagatga ttgaatatgg aactcgcgtg atcggcggcg tcactcccgg
     1681 aaaaggcgga cagaagagcg agcacggagt gcccattttc aatacgcttc aggatgcagt
     1741 ggacaaagag ggcgcgaacg ctacagtgat ttttgttcca cctccattcg cagcggatgc
     1801 gattctagaa gcagtcggtg ctcagattcc tctttgcatt tgcattacag agggaatccc
     1861 ggtgcgcgat atgtcccgcg tatattctgt tctcaagact tcaaagactc gcctgattgg
     1921 accaaactgt ccaggtgtca tcagccctgg caaatgcaag atcgggatta
//
LOCUS       DAORFF010000656         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_47959_length_1952_cov_4.558250, whole genome shotgun sequence.
ACCESSION   DAORFF010000656 DAORFF010000000
VERSION     DAORFF010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47959_length_1952_cov_4.558250"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="PKK76_18760"
     CDS             <1..992
                     /locus_tag="PKK76_18760"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNL70576.1"
                     /translation="GDDIMDGIAINHVYTVEPAGSFLKDESKVERDGAWAVHYAMARL
                     LSGKFRTAMVVAFSKASQCTYSAFSGMSADPFYLRPVGADGDSIAALQANYFMQRTGA
                     TEADLAQVARKNRANGSANQSRTMEGETKDFSIDEILKSARIATPITRLTAAYPGDGC
                     VVMILATEEYVKEKKRNAAWITGVGLSGDAYYPTYRNLSELKHVGLAANKALEMSGRK
                     IGDVNIFELQENYAHQELMLCEALGIAPAGSPQKALNDGAFDRSSGKPVNASGGALSG
                     GIIYASGLARLMEASLQVTGQAGPSQVPGAKTALVSAQAGLGIQSSIIYVVEG"
     gene            994..>1952
                     /locus_tag="PKK76_18765"
     CDS             994..>1952
                     /locus_tag="PKK76_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70577.1"
                     /translation="MIRAWCLLILLFSLQVYSEPTELERYLEWAKYPPNSRPLEQTQV
                     DLLDPYNSDRPPVPVSGLSAACTLIPQSAVSVGTKEFKIYLHCKSSLGQPLKVDSVKA
                     RVYTEDRGKQKASAAPRIDAGNRPDQSIQFLVKPGVQDWGDMFLEADFNVQGKRHNQR
                     AHWFSTPHNVAEFKQGATESVRQGHLYVRIPVWIHKAGYFAFDANLFSKSGPVATSSV
                     EGDYSAGLQTIEFQFFGKVIRDKKAGGPFTIRNIRGMRNNSPVTPSMVRDSINGKPLP
                     PHKEQTEPWQEFMEPFAGAVTTAPHSLAVFSSLEWQSPEKTRR"
BASE COUNT          500 a          493 c          526 g          433 t
ORIGIN      
        1 caggcgacga catcatggac ggaatcgcca tcaaccacgt ctacacagtg gaaccggccg
       61 gttcatttct aaaagacgaa tccaaagtag agagagatgg agcgtgggcc gttcattacg
      121 cgatggctcg tcttctcagc ggaaaatttc gcactgccat ggtagttgca ttttctaaag
      181 caagtcaatg tacgtattca gcgttcagcg gaatgtccgc ggatccattc tatctcaggc
      241 cagtgggcgc ggatggcgat agcatcgctg cgctccaggc aaattacttc atgcagagga
      301 ccggcgcaac ggaagcagat ctggcgcaag tggcccgcaa gaacagagca aacggatccg
      361 cgaatcagtc gcgaaccatg gaaggggaaa caaaagattt ctccattgat gaaattctaa
      421 aatctgccag gatcgccact ccgattacgc gattgactgc ggcctatcca ggagatggtt
      481 gcgttgtcat gattctcgca acggaagaat acgtgaaaga aaagaaaaga aacgctgcat
      541 ggatcacagg tgtaggactg agcggggatg cgtattatcc cacttacaga aatctttctg
      601 agctcaaaca cgtgggcctc gcagccaaca aagcactcga gatgtcgggc agaaaaatag
      661 gcgatgtgaa catttttgaa cttcaagaaa actatgcaca ccaggaactt atgctgtgcg
      721 aagccctggg catcgcgcca gccggaagcc cgcaaaaagc actgaacgac ggtgcgtttg
      781 atcgctcttc gggcaaaccg gtgaatgcat ctggcggcgc tctctccggc ggcatcatct
      841 acgcatcggg ccttgctcgc ctcatggagg catctcttca ggtgaccggg caggcgggcc
      901 catcgcaggt tccgggcgcg aagactgcgc ttgtttctgc gcaggccgga cttggaattc
      961 agagcagcat catttacgtc gtagaaggtt gaaatgattc gggcgtggtg cctgctgatt
     1021 ctgctgttca gtcttcaggt gtactccgaa ccaacggagc ttgaacgata tctggaatgg
     1081 gccaaatacc cgcccaacag tcgtccgctg gaacaaacgc aggtagatct actcgaccct
     1141 tataattctg atcgtccgcc tgtgccggtt tctggattga gtgctgcgtg cactctgatt
     1201 ccgcagagcg ccgtgtccgt tggaacaaaa gaatttaaga tctatcttca ctgcaaatct
     1261 tcgctgggac agccgttgaa agtagattcg gtgaaggctc gcgtatacac ggaagaccgc
     1321 ggaaaacaaa aggcctccgc cgcgcctcgc attgatgccg ggaacagacc agatcagagc
     1381 attcaatttc tggtgaagcc aggcgtgcag gactggggcg atatgtttct tgaggctgac
     1441 tttaatgttc agggtaagcg tcacaatcaa cgtgcgcact ggttttcaac gccgcataac
     1501 gtcgcagaat tcaagcaagg ggccaccgaa tcagtgcgcc agggccattt gtatgttcgc
     1561 atccctgtat ggattcacaa ggcaggatat tttgcttttg atgcaaactt gttttcgaaa
     1621 tcaggaccgg tggcgacttc gagtgtggaa ggagactatt ccgccgggct tcaaacgatt
     1681 gaatttcaat tctttggtaa agtgattcgc gataagaagg caggcggccc gtttaccata
     1741 agaaatattc gcgggatgcg aaacaactct cctgtgactc cttccatggt gcgagattca
     1801 atcaatggaa aacctctccc acctcacaaa gagcagacag aaccatggca ggaattcatg
     1861 gagccttttg ccggggccgt gacgactgcg ccacattccc ttgccgtttt ttcctcgctc
     1921 gaatggcaat ctccggaaaa aacccggaga at
//
LOCUS       DAORFF010000657         1950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_48073_length_1950_cov_2.959367, whole genome shotgun sequence.
ACCESSION   DAORFF010000657 DAORFF010000000
VERSION     DAORFF010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48073_length_1950_cov_2.959367"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PKK76_18770"
     CDS             complement(<1..323)
                     /locus_tag="PKK76_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70578.1"
                     /translation="MNAILFPLFAIWGIFSIVLLFQKRIEWHLRISAMLICAFYIVLF
                     YSEAHQSFESYRDSFRTTLGALLTSVLPFVGLLLLVFWPFVILISFYSIHASLARGLL
                     RIFTVL"
     gene            complement(320..1288)
                     /gene="pdxA"
                     /locus_tag="PKK76_18775"
     CDS             complement(320..1288)
                     /gene="pdxA"
                     /locus_tag="PKK76_18775"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNL70579.1"
                     /translation="MTGGDPAGIGPEIFLGSLPEILKKHPIIYINTAGAQHARECVRI
                     AEGVHVRTLVVSSESLREAGSGLTILDLPEQERVVPGIPGRESGRRALAALEAACIWI
                     KQRGTKALVTMPLSKHHVSLSHPAFRGHTDYLADEFSSKVIMLMHGKALSVIPLTVHV
                     PLSHVPAELHARMNDADLIRILKILHGWKEYSGTRWALCGLNPHAGENGLLGREEIDF
                     MNIIADRWRAEGLPIEGPLPADGIFQEYHHGRYRLVLSAYHDQGLAPFKALEGRAGVN
                     CTIGLPFVRTSPDHGTAFEIAGKGGADSASFAAAFELAQASEPLWI"
     gene            complement(1309..1746)
                     /locus_tag="PKK76_18780"
     CDS             complement(1309..1746)
                     /locus_tag="PKK76_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNL70580.1"
                     /translation="MALVKTASEVTNSTIGENSYFSGRFFINGSLKIDGKFEGKSLQA
                     DQLYIGVTGRVKTNVTASSVIVEGIIVGNITARNRVMLLPTARILGDISTPELIIQNG
                     VILEGRCMISNDLKHSARDYIESEYGRDNLTLERLFGTRARTA"
BASE COUNT          512 a          477 c          496 g          465 t
ORIGIN      
        1 agcacagtaa aaatgcgaag gagtccgcgc gcgaggcttg cgtgaatgct gtagaaagaa
       61 atcaaaatga cgaatggcca gaataccaga agcagcaagc ctacaaatgg caatacagaa
      121 gtgagcagag cgcctaacgt agtacgaaac gaatctctgt agctctcaaa cgattgatga
      181 gcctccgaat agaaaaggac aatataaaaa gcacaaatca gcatggcact gatgcgaaga
      241 tgccattcga ttcttttctg aaaaagaaga actatggaga agattcccca gatcgcaaaa
      301 agtggaaaga gaatcgcgtt catatccata gcggttctga agcctgggca agctcaaaag
      361 cagccgcaaa ggaagctgaa tctgcccccc cctttcctgc aatctcaaat gcggttccgt
      421 gatcgggact tgtgcgaaca aacgggaggc caatcgtgca attcacgcca gccctgcctt
      481 ccagagcttt gaatggagca aggccttgat catggtaggc agaaagaacc agcctgtaac
      541 gtccgtgatg gtattcctga aaaatcccat cggctggaag agggccttcg atgggcaaac
      601 cttctgctct ccagcgatct gcaatgatat tcataaaatc gatctcttcg cggccaagaa
      661 gtccgttttc tcccgcatgt ggattgaggc cgcacaaagc ccatctcgtg ccagagtact
      721 ctttccaacc gtgaaggatt ttcaaaatgc ggatcaaatc cgcatcgttc attcttgcat
      781 gcaactcagc cggcacgtgg ctcagcggta catgaactgt aagtggaatg acactcaaag
      841 cttttccatg catcaacatg atcaccttgg aactgaattc atccgccagg taatctgtgt
      901 gtccgcgaaa tgcgggatga gacaaactca cgtgatgctt ggaaagaggc atggtgacga
      961 gtgctttggt tccacgttgt ttgatccaga tgcacgcagc ttcaagcgcg gcaagcgctc
     1021 gccttccgga ttctcggccg ggaattccag gcactactct ttcctgctcc ggtagatcta
     1081 aaatcgtaag cccactgcct gcttcacgca aactctccga ggaaacgaca agggtgcgca
     1141 cgtggacgcc ttctgcgatg cgaacgcact ctcttgcatg ttgtgcgcct gcagtgttga
     1201 tgtaaatgat ggggtgcttt tttagaatct ctggaaggga gcccagaaag atttccgggc
     1261 caatgccggc aggatcaccg ccggtgatga cgatgtgctc tttcagactt acgcagtcct
     1321 cgcacgtgtg ccgaacaatc tttcaagagt gaggttgtct ctgccatact ctgattcaat
     1381 gtaatctcgc gcagagtgtt tgagatcatt ggagatcatg caacgacctt cgaggatcac
     1441 accgttctgg atgatgagtt ccggagtaga aatatctccc aggatacgag ctgtggggag
     1501 aagcatgacg cgatttcgtg cagtgatgtt gcctacaatg attccttcta cgatgacaga
     1561 agatgcagtt acgtttgttt tgacccggcc cgttacaccg atgtagagct gatctgcctg
     1621 gagagacttg ccttcgaatt ttccatcaat cttgagtgat ccgttgatga agaatcggcc
     1681 actgaagtac gaattctcgc cgatcgtgga gtttgtgacc tcagatgctg tcttgacaag
     1741 tgccattgag ccagagaagc ggactcgccg tgattcctca atcagaattt gggaaaggaa
     1801 tcagggtttc actttgaact ggggcaaatt cagcctttct atgaccttcg aagtaaaata
     1861 ttcgacttcg cggatcgcgt cagcgatggt tcggccctcc agatagttgg actcctcgaa
     1921 acggctgttg gtcttgatga cttcatcccg
//
LOCUS       DAORFF010000658         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_48161_length_1948_cov_2.760697, whole genome shotgun sequence.
ACCESSION   DAORFF010000658 DAORFF010000000
VERSION     DAORFF010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48161_length_1948_cov_2.760697"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1948
                     /gene="mgtA"
                     /locus_tag="PKK76_18785"
     CDS             <1..>1948
                     /gene="mgtA"
                     /locus_tag="PKK76_18785"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HNL70581.1"
                     /translation="EVLLAQNGPNEIATEKRESSIRRFLTSLRNPLVLLLLFLATVSY
                     ATGDLRATIVILVMVLLGVVLRFFQEERADRAAEKLKKLVGSTATVIRDGVPHEIPFA
                     KLVVGDLVHLAAGDMVPADVRVVQAKDLFLNQAALTGESMPVEKKPVNVPSQNTPDAP
                     DLCFLGSSVESGSASAVVVTTGARTYLGQLASSIAGSSPATSFDRGINQFTWLMIRFI
                     LVMVPTVFLLNGFGKHNWLDAFLFAVAVAVGLTPEMLPMIVTVNLSRGAIAMSRKKVI
                     VKRLAAIQNFGAMNILCTDKTGTITRGKIILERYLDVRGHESERVLHYGYMNSYYQTG
                     LKNLLDEAVLSYGHLEEDLKVKEGYRKIDEIPFDFTRRRMSVIVEEKVNQHILICKGA
                     VDEVMHLSTHAEIEGHVVPLQAEHHAHRTRLVRSLNSQGFRVVAVAYKTMNGDNDEPH
                     YGVADESDLTLLGYLAFLDPPKESASEALDKLSHLNVDVKVLTGDSELVAQYVCNHVG
                     MKTERILLGPEIEKLTDDELASVASGTTLFARLAPVQKERIIRALQKAGNVVGFMGDG
                     INDSPALKAADVSISVDSAVDIAKESSDIILLEQSLLVLETGVREGRRVFGNIIKYIK
                     MAASSNFGNMFSVVGASAFLPYLPM"
BASE COUNT          493 a          482 c          517 g          456 t
ORIGIN      
        1 agaagtgctc ctggcgcaaa atgggccaaa tgaaattgcc actgaaaagc gcgagtccag
       61 catcagacga ttcctgacga gcctgcgaaa ccctctcgtc ctgctccttc tgtttttagc
      121 gactgtttcc tacgcaaccg gcgatcttcg cgcaaccatt gttatcctgg tgatggtgct
      181 cctgggcgta gtacttcgtt tttttcagga agagagagca gaccgggctg cggaaaaact
      241 caagaaactt gtgggctcga ccgcaaccgt gattcgcgac ggtgttcctc atgaaatccc
      301 attcgctaaa ctggtggtag gcgatttagt tcatctggcc gcgggtgata tggttccggc
      361 agacgtgcgc gtggtgcagg caaaggatct ctttctaaat caagccgcac tcaccggcga
      421 atccatgccg gttgaaaaaa aaccggtgaa cgttccatcg cagaacacgc ccgacgcgcc
      481 ggacctttgc tttcttggat cgagcgtgga gagtggatct gcctccgcgg tcgtagtcac
      541 aacaggagct cgcacgtatc tgggtcagct ggcaagcagc atcgccggaa gttcaccagc
      601 gacgagtttt gaccgtggaa tcaaccagtt cacctggctt atgattcgct ttatcctggt
      661 gatggtgccg acggtctttt tgctcaatgg ctttggcaaa cacaactggc tagacgcatt
      721 cctgtttgcg gttgctgttg ccgttggtct gactccggag atgcttccaa tgattgtaac
      781 tgtgaatctt tcgcggggtg ctatcgccat gtccagaaag aaggtcatcg taaagaggct
      841 ggccgcgatt caaaattttg gcgcgatgaa cattctctgc acagacaaga caggaactat
      901 tacgcgcgga aaaattattt tggaaaggta cctcgatgtg cgaggacatg aaagcgaacg
      961 ggttctgcac tacggataca tgaacagcta ctaccagact ggactgaaga atctattgga
     1021 tgaagcggtt ctatcctacg gccacctgga agaagatctt aaagtaaaag aaggatatcg
     1081 taaaattgat gagattccct tcgatttcac gcgaaggcga atgtctgtga tcgtagaaga
     1141 aaaagtcaat cagcacatcc tgatctgcaa gggtgccgtg gatgaggtga tgcatctatc
     1201 cactcacgca gaaatcgaag gacatgttgt ccctcttcaa gctgaacacc atgcccacag
     1261 aaccagactg gttcgatccc tgaattcgca aggctttcgc gtagttgcag tagcttacaa
     1321 aaccatgaac ggcgacaacg atgaaccgca ttacggggta gcagacgaaa gcgacctgac
     1381 tcttctgggt tacctcgcat tccttgatcc gcccaaagaa tcagcttcag aagcgctcga
     1441 caagctctca cacctgaacg tggacgtcaa agttctcacc ggagatagtg aacttgtggc
     1501 tcaatatgtg tgcaaccatg ttggaatgaa aacagagcgc attctgctgg gtccggaaat
     1561 cgaaaaactc acggacgatg agcttgcatc tgtggcatcc gggaccactc ttttcgcgcg
     1621 cctcgcaccc gtgcaaaaag agagaatcat acgagcgtta caaaaagcgg ggaatgtagt
     1681 tggcttcatg ggggatggga tcaacgattc tcctgcgctc aaagccgcgg acgtgagcat
     1741 ttcagttgat tctgcggtgg acattgcgaa ggaatcgtcg gatatcatct tgctcgaaca
     1801 gagtctgctg gttctggaaa ccggagtgcg agaaggtcgt cgagtcttcg ggaacatcat
     1861 caagtacatc aagatggcag ccagttccaa cttcggcaac atgttcagcg tggtgggagc
     1921 aagcgcattt cttccctatc tccccatg
//
LOCUS       DAORFF010000659         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_48588_length_1939_cov_2.844480, whole genome shotgun sequence.
ACCESSION   DAORFF010000659 DAORFF010000000
VERSION     DAORFF010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48588_length_1939_cov_2.844480"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PKK76_18790"
     CDS             <1..546
                     /locus_tag="PKK76_18790"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNL70582.1"
                     /translation="IELTKPGIQEAHFSLFYPALGPDHPDRYKQDLMNYLLGGNESGL
                     LFERIREELGLSSYGIYSYVMRTPGHAVLGISGGIAPDELHLMEKEVKSCIARICESK
                     LEEHRLHRARASLRTSIASYAETSSGLNGMIGLPALRGDTSHPVEKALSEIEKITLED
                     VHEMAQKTFSGPQFRAVLTPA"
     gene            543..1538
                     /locus_tag="PKK76_18795"
     CDS             543..1538
                     /locus_tag="PKK76_18795"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNL70583.1"
                     /translation="MKVDLNTASDILLSGNIVAIPTETVYGLAADALNPDAVLQIFEA
                     KKRPRFNPLICHLPDVADVKKYCHPPACLEVLASLWPGPLTILMPHNGSIPSLVTAGS
                     SLCAFRVPSHPIALELLKKVRRPLAAPSANKSGRVSPVNAEMVEREFRGSLPVLDGGQ
                     CSVGLESTIVLANEDSVSILRNGKYTALDFENLGLKVEPPAEDIRAPGQMLRHYAPEV
                     PLILLTVERKRVESEAMHVDAKSKIAWLGFRGSKPPFDAQVFELSQSGDLHEAARNLF
                     FYFDRMARERFDYIVAELVPEEGIGLAINDRLRRAADMEGNVQNERLSAWRIMHV"
     gene            1531..>1939
                     /locus_tag="PKK76_18800"
     CDS             1531..>1939
                     /locus_tag="PKK76_18800"
                     /inference="COORDINATES: protein motif:HMM:NF013390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HNL70584.1"
                     /translation="MFESLVKFFYDLQDQITGALEQESGSSKFHEDTWTGEGWLAGGG
                     RTRVMESGTVLERGGVNVSDVHGLLPDDLARNMPGKAREFRAAGISLVLHPLNPHAPT
                     VHANFRIISRGKQEDPETIWFGGGADLTPMILYE"
BASE COUNT          508 a          487 c          508 g          436 t
ORIGIN      
        1 atagaactca cgaagcctgg aattcaagaa gcacactttt ccctttttta tcccgcgctc
       61 ggtccagatc atccagacag atacaagcaa gatttgatga attaccttct gggtggaaac
      121 gaatccggct tactctttga acgaatcaga gaagaactgg gactatcaag ctatgggatt
      181 tattcctacg ttatgcgcac acccgggcat gctgtacttg gcatttctgg aggaatcgcg
      241 ccggacgaat tgcacctaat ggaaaaagaa gtgaaatcgt gcattgcacg gatctgcgaa
      301 tccaaactgg aagaacacag acttcatcgc gcgagagcct ctcttcgcac gtcgattgca
      361 tcctatgcag aaacgtcaag cggactcaat ggaatgattg gactgcctgc actcagaggg
      421 gacacttctc accctgtaga aaaagcactc tctgaaatag aaaagatcac actggaagac
      481 gtgcatgaga tggctcaaaa aactttctcc ggtccgcaat tccgggccgt tctcactccg
      541 gcttgaaggt tgatctaaac acagcatcag acatactatt gtctggaaat atcgttgcca
      601 ttccaaccga aacggtctat ggactggcag cagacgctct aaatccagat gcagtactgc
      661 aaatcttcga agcaaagaag agaccgaggt tcaacccttt gatctgtcat ctgcctgacg
      721 tagcggacgt taaaaaatac tgccacccac ctgcttgcct ggaagtactg gcgtctctat
      781 ggcccggtcc tctgaccatt ctaatgccgc ataacgggtc tattccctct cttgtaacag
      841 ctggctctag tctatgtgct tttcgagttc cttcccatcc gatcgcgctt gagctactta
      901 aaaaagtgcg aaggcctctg gccgctccat cagcgaacaa aagcggacgc gtgagcccgg
      961 tgaacgcaga aatggtggaa agagaattta gaggatcgct tccagtcctc gacggaggcc
     1021 agtgttccgt aggacttgag tctacgattg tcctggcaaa tgaagacagc gtttcgattt
     1081 tacgaaatgg caagtacaca gctcttgatt ttgaaaacct gggactgaaa gtggaaccgc
     1141 ctgccgagga catccgtgcg ccaggacaaa tgctgcggca ctatgcgcct gaagttccgc
     1201 tcattctgct tacagtggaa cgaaagagag tagagtcaga agcaatgcat gtggacgcga
     1261 aaagcaaaat cgcctggctg ggttttcggg gaagcaaacc tccgtttgac gctcaggtgt
     1321 ttgaactctc gcaaagcggg gatctgcacg aagcagcgcg caatctattt ttttactttg
     1381 accgcatggc ccgggaacgc ttcgattaca tcgtcgcaga gctcgtcccg gaagaaggaa
     1441 tcgggcttgc gatcaacgac agactgagac gcgcagcaga catggaaggc aatgtgcaga
     1501 atgaacgttt gtctgcctgg agaatcatgc atgtttgaga gcctggtgaa atttttctat
     1561 gatttacagg atcaaatcac aggtgcgctc gaacaggaat ccggatcttc gaagttccac
     1621 gaagacacct ggacgggcga aggctggctg gcgggcggcg gtagaaccag agtcatggaa
     1681 tctgggacgg ttctcgaacg cggaggcgtg aatgtttccg acgtgcacgg acttctcccg
     1741 gatgatctgg cgcgcaacat gcctggaaag gcacgcgagt ttcgtgcggc gggaatttct
     1801 ctggtgcttc atccgttgaa tcctcatgcg cccacagtgc acgcgaactt cagaatcatc
     1861 agccgcggaa agcaggaaga cccggaaaca atatggtttg gcggcggggc ggacctcacc
     1921 cccatgattc tctatgaag
//
LOCUS       DAORFF010000660         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_48783_length_1935_cov_2.539362, whole genome shotgun sequence.
ACCESSION   DAORFF010000660 DAORFF010000000
VERSION     DAORFF010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48783_length_1935_cov_2.539362"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /gene="metX"
                     /locus_tag="PKK76_18805"
     CDS             complement(<1..202)
                     /gene="metX"
                     /locus_tag="PKK76_18805"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772219.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNL70585.1"
                     /translation="MQSEDLQDSGRRDYLSPLGRLDPTVVRLPGITTQSGYTFPYIEL
                     SYLTIGKLSPAKDNAILICHALS"
     gene            complement(203..1504)
                     /locus_tag="PKK76_18810"
     CDS             complement(203..1504)
                     /locus_tag="PKK76_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNL70586.1"
                     /translation="MSMNQLETIALHAGQVPDPVTKSRAVPLYQTTSYVFDDADHAAR
                     LFGLQEFGNIYTRIMNPTTDVLEKRIAALEGGVGALATASGQSAETLAILNAAEAGDE
                     IISSSSLYGGTYNLFHYTLPKMGIQVKFVDPSKPEDFKKAITAKTRLIFAETLGNPKL
                     DVLDFEAVSKIAHAEGLPLFIDNTLASPFLLRPFDHGADIVVHSTTKFIGGHGTSIGG
                     IIVDSGKFAWNQGRHKMFTEADESYHGLKFWDVFGKFEPFGGVNIAYILKARVQGLRD
                     IGAAMSPFNAWMFLQGLETLHLRMERHSQNAMKVAQFLKSHPKVSWVNYPGLPDHPSY
                     NLAKKYFYRGLFGAILGFGIKGGKEAGKKFINNLKLFSHLANVGDAKSLAIHPATTTH
                     SQLTAAEQVATGVSDDFVRLSVGIEGSEDIEADLKQALDKI"
     gene            complement(1551..>1935)
                     /locus_tag="PKK76_18815"
     CDS             complement(1551..>1935)
                     /locus_tag="PKK76_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70587.1"
                     /translation="QAQWDGDALRFRLKSMSEAFGLAQELLQFVRRTSLHQQTQGRRW
                     VTSVCVVSEGSLFQLAGAPEMPLALLRGPLREDHEQFIRSLTDRDLEILTGEFLPVVP
                     ASFHPPGLERRPFGDWAIAHRPGGS"
BASE COUNT          467 a          513 c          479 g          476 t
ORIGIN      
        1 cgctgagcgc atgacagatc aggatcgcgt tgtctttcgc gggcgaaagt ttaccaatcg
       61 ttaaatagga cagttctatg tacggaaacg tgtaacccga ctgcgttgtg atcccgggca
      121 atcgaactac agtcgggtcg agtctgccaa gcggcgacag gtagtcacgc cgcccggaat
      181 cttgaagatc ttcgctctgc aattagatct tgtccagagc ctgcttgaga tccgcttcta
      241 tgtcttcgga gccttctatt ccgactgaca accgaacgaa atcgtcagac acaccagtgg
      301 ctacctgttc cgccgccgtc agctgcgaat gagtggtggt ggcagggtga atggcaagac
      361 tctttgcgtc tcccacgttt gccagatgtg agaaaagctt cagattattt atgaattttt
      421 ttcctgcctc ttttcccccc ttgattccaa atccaagaat ggctccaaag agacctctgt
      481 agaaatactt cttcgccaga ttgtaggaag gatgatccgg aagtccagga tagttaaccc
      541 aggatacttt tggatgtgac ttgaggaatt gcgccacttt catcgcattc tggctgtgtc
      601 tttccatacg aagatgcaga gtttcgagcc cctggaggaa catccaggca ttgaaaggag
      661 acatggccgc tcctatgtcg cgcaaacctt gcactcttgc cttgagaatg taggcaatgt
      721 tgactccgcc gaatggttcg aacttaccaa acacatccca gaatttcaga ccgtgatagg
      781 attcatccgc ctctgtgaac attttgtgac gaccctggtt ccacgcgaac ttaccggaat
      841 cgacgatgat cccgccaatg gaagtaccgt ggccaccgat gaattttgta gtggaatgaa
      901 ctacaatgtc cgctccatga tcgaacggac gcagaaggaa cggggaagcc agagtattgt
      961 caatgaagag aggcagacct tccgcatgtg cgattttgct cacagcttca aaatcaagca
     1021 catccagttt cggatttcca agcgtttccg cgaaaatcaa acgggtcttc gcagtgattg
     1081 cttttttgaa atcctccggt ttagacggat ctacaaactt cacctgaatg cccatttttg
     1141 gtaaagtata gtgaaacaga ttgtatgtac caccatacaa actggaactg gaaatgattt
     1201 catctcctgc ttccgctgca ttcaagatgg ccagggtttc agcagactgt ccggatgcag
     1261 tagcaagagc gccgactcca ccttcgagtg ccgcgatgcg cttttcgagc acatctgttg
     1321 ttggattcat gatgcgagtg tatatgtttc caaactcctg cagaccgaac aatcgagccg
     1381 cgtgatctgc gtcgtcgaag acatacgaag tggtctggta gagcggaact gcccttgatt
     1441 ttgtgactgg atcaggcacc tggcctgcgt gcaatgcgat ggtttctagt tgattcatac
     1501 tcatatggta accctcaatt ccttgttgcg cgactcgtgt aaaccctttc tcaagaaccc
     1561 ccgggtcgat gcgcgatcgc ccagtctccg aaaggcctgc gctccaatcc gggagggtga
     1621 aaggacgcag gtacgacagg caagaattcc cctgtcagta tctccaggtc cctgtccgta
     1681 agagatcgaa tgaattgttc gtgatcctcc ctcaatggcc cgcgcaagag cgcgagaggc
     1741 atttcaggtg cgcccgccag ctgaaagaga gatccttcgc tgacaacaca cacggaagtt
     1801 acccagcgcc tcccttgcgt ctgctggtgc agactggtcc ttctcacaaa ctgtaagagc
     1861 tcctgtgcca ggccaaaggc ttccgacatc gacttcagac gaaaccgcag ggcatctcca
     1921 tcccactgcg cctgc
//
LOCUS       DAORFF010000661         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_48871_length_1933_cov_3.202343, whole genome shotgun sequence.
ACCESSION   DAORFF010000661 DAORFF010000000
VERSION     DAORFF010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48871_length_1933_cov_3.202343"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1343
                     /locus_tag="PKK76_18820"
     CDS             105..1343
                     /locus_tag="PKK76_18820"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70588.1"
                     /translation="MYRALFVVSILTLSYCASTPDEQQQPQKQADRKLSLSCSGGAAV
                     LTRVDSKGKPGERGQVDPKPMTCEAASKLDDKGLSKLKRIGKWEYFVDSVLSRREEYV
                     AGLKEGPETGYFPDGSIRYTGQNSADKKTGHWKIKADATKGCESEGDFENDLKTGRWV
                     ECKGNIKEPFIEFEGSYVQGLRDGPGKFLNSEGKVSSQGDFRADLGCKAALKPGASKQ
                     DFDRCAKRTGHWVYYHSNGEKSTEGDFDPATGLETGTWHEFYRSGEKLGMGPRTGDRK
                     GLWTFYGKDGSILYQLEFAGSDFLPKTAIFWKDGKKSAQGPLSMGMFKFSQDKDAVEL
                     TSPIKDGIWTEYYPNGQKSGEGNYTSNRRDGKWTLYDESGKKIGEGPYMMDKKDGEWL
                     ELEGGAMVKKKYQFGKPARF"
     gene            1340..>1933
                     /locus_tag="PKK76_18825"
     CDS             1340..>1933
                     /locus_tag="PKK76_18825"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNL70589.1"
                     /translation="MIRYFVTLTFSILFFQTAAFSHPASLLVAEEGGRVFVSENTAIV
                     RPMASLTKIGSALFAVSLSPLDRWIRLSETVRSRGPDDRSAPLRLQEQYTLRDMLSAM
                     LISSTNAASRSVAKELAGSESEFVRRLGEFGARRGWKQTRFADSHGLSPASISSVDDI
                     SRMMDDFIRNPDLMKVLESVNPVIHEKQGRTIGLLSTL"
BASE COUNT          510 a          435 c          546 g          442 t
ORIGIN      
        1 tctatttctt ctccccgcac agatgtgcgg gaccaggatc gcggccaggc cgtggatact
       61 tttgcctgaa tgtcgtataa cgtttcaaaa tccggagatt tctaatgtat agagccttat
      121 tcgttgtttc cattcttact ttgtcctatt gcgcgagcac acccgatgaa cagcagcaac
      181 cgcagaagca ggcagatcgc aagctgtccc tttcctgcag cggaggtgcg gcggttttaa
      241 cacgggtaga ttcaaaagga aaacccgggg aaagaggtca ggtagatcca aagccaatga
      301 cctgtgaggc ggcctcaaag ctggatgaca aaggactttc gaagctcaag agaattggga
      361 agtgggaata cttcgtagat agcgttctct ccagacgtga agaatatgtg gcaggtctaa
      421 aagaaggtcc tgagacagga tattttccgg acgggtccat tcgttacacg ggtcagaact
      481 ctgcggataa gaaaaccggg cactggaaga tcaaagcgga tgcgacgaag ggatgtgaat
      541 cagaaggcga ttttgaaaac gatttgaaaa caggtcgctg ggtggaatgc aagggaaaca
      601 tcaaagagcc atttatcgaa tttgagggaa gctatgttca gggcttgcgc gacggtcctg
      661 gtaaattcct gaacagcgag ggcaaggtga gttcgcaagg agatttccgt gccgatcttg
      721 gctgcaaggc agcgctgaag cccggtgcaa gcaagcagga ctttgacaga tgcgcgaagc
      781 gcacaggtca ctgggtgtac tatcattcaa atggagagaa atcaaccgaa ggggattttg
      841 atccggccac aggtctggaa acaggaacat ggcacgaatt ctatcgtagc ggtgaaaagc
      901 tggggatggg tcctcgcact ggggatcgga agggcctgtg gacgttttac gggaaggacg
      961 gttcgatctt atatcaactt gagtttgcgg gaagtgactt tctgccaaag actgccatct
     1021 tctggaaaga cggcaaaaaa tccgcgcagg gtcctctttc gatgggcatg ttcaaattca
     1081 gccaggacaa agacgcagta gaacttacta gccccatcaa agatggaatc tggacagagt
     1141 actatccaaa cgggcagaaa tcaggagaag ggaattatac cagcaatcgc cgtgatggga
     1201 aatggacgtt atacgacgaa tcaggaaaga aaatcggaga aggtccatac atgatggata
     1261 agaaagatgg agaatggctg gagcttgaag gtggagccat ggtgaagaag aaatatcagt
     1321 tcgggaagcc cgctcgtttt tgattcggta tttcgtaact cttactttct caattctgtt
     1381 ttttcagacg gccgcgtttt cgcatccggc ttcgcttctt gttgcagagg agggtggccg
     1441 ggtcttcgtc tcggagaaca ctgcaatcgt caggcccatg gcgagcctca ccaaaatcgg
     1501 ctctgctctt ttcgctgtga gtctatcccc tctcgatcgc tggatccgcc taagcgaaac
     1561 agtgcgaagt cgcggaccag atgacagaag tgccccgctt cgtttgcagg aacaatacac
     1621 tctgcgcgac atgctatctg cgatgctcat aagctcaacg aacgctgcat ccagatctgt
     1681 tgctaaagaa ctggcgggtt cggaatcaga attcgttcgc aggttaggcg aatttggggc
     1741 tcgtcgcggc tggaaacaaa ccaggtttgc ggacagccat ggcctttctc ctgcttctat
     1801 ttcttccgtg gacgacatat ctcgcatgat ggacgacttc attcgaaacc ctgatttgat
     1861 gaaagtgctc gagagcgtga atcctgtgat ccacgagaag cagggccgca cgatcggact
     1921 tctgtctacc ctg
//
LOCUS       DAORFF010000662         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_49124_length_1928_cov_3.294714, whole genome shotgun sequence.
ACCESSION   DAORFF010000662 DAORFF010000000
VERSION     DAORFF010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49124_length_1928_cov_3.294714"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PKK76_18830"
     CDS             complement(<1..919)
                     /locus_tag="PKK76_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70590.1"
                     /translation="MRTLIACILLALASCSVRTGEAEQGVLDLRSWDSQSDVISLDGE
                     WLFAPGLSPRPADFQVVRLPADSDFHSQKIETGCVTLKLKLRLPESVNKVGLISGKQL
                     SSHRILWNERVVAGAGTPACSAPEYEPSLEPSVSFWNVKGGDSEIVLQIANFDNYEIS
                     AGRILLGSEKTAQTFLLRRVAGEIVTLGALIIVAFYHLGLFALRRRELSHLMVGVLCI
                     TGLAHFASSGIHLVPLYLNLPYSMYVRTDYISWFFAGSVFVHYTVLALRLTPPVWIVP
                     ANYATSVALTLFTLFTKPILFTQITPFFEV"
     gene            complement(1308..1928)
                     /locus_tag="PKK76_18835"
                     /pseudo
     CDS             complement(1308..1928)
                     /locus_tag="PKK76_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
BASE COUNT          472 a          481 c          497 g          478 t
ORIGIN      
        1 cgacttcaaa gaatggagtg atctgggtga acaggattgg ttttgtaaaa agagtaaaga
       61 gagtcagagc aacggatgtg gcatagtttg ccgggacaat ccacacagga ggagtcaggc
      121 gcaaagcgag caccgtgtag tgcacaaaaa cagatcccgc aaaaaaccac gatatatagt
      181 ccgtgcgaac gtacatggag tatggcaggt tcagatagag tggcacgaga tgaattccgg
      241 aagatgcaaa atgagcaagc cctgtaatac aaagaactcc taccatcaaa tgcgaaagct
      301 cgcgtctcct gagtgcgaaa agccccagat ggtagaacgc aacgatgatc aaagcgccga
      361 gggttacaat ttcccccgcc actcttctca aaagaaaagt ttgagctgtc ttttcggaac
      421 caaggagaat tctccccgct gagatttcgt agttatcaaa attcgcaatc tgtaagacaa
      481 tttcagaatc tccacctttc acattccaga aagatacgga tggttcgaga gaaggctcat
      541 actcaggtgc agaacaggcg ggcgttcccg caccggccac gacgcgttcg ttccagagga
      601 tgcgatgaga gctcaattgc ttccctgaaa tcaaacccac cttattcacg ctctcaggca
      661 gtcgcagttt gagtttcaga gtgacgcagc cggtttcgat tttttgagaa tggaaatcgc
      721 tgtccgcggg cagtcggacg acctggaagt cagccgggcg cggagatagc cccggggcga
      781 ataaccattc gccgtccaga ctgatgacgt cgctctgaga atcccaggat ctgagatcga
      841 gaacaccctg ctccgcttca cccgtgcgga cggagcaaga ggcaagagcc agaaggatgc
      901 atgcgatcag cgtgcgcatg ttcgatgaac gcaagaaacg accacccgcg tcagccattt
      961 tccacccggg cgatgcatga tttttgtctt acaaaaatca gcgggaaatt ctatttcttc
     1021 cggcttgtct ttgacttcgt agctgtcttt gacttcgtag ctgtctttga cttcgtagct
     1081 gtctttgact tcgtagcttg ctttgatttc gcggctggct ttgacttcgc ggctggcttt
     1141 gatttcgcgg ctggctttga tttcgcggct ggctttgact tcgtagctgg ctttgatttc
     1201 gccaatggct gtggtcttgc agcaggcgct ttctctggac ttgcacctgc ctcgatggcg
     1261 aagcccggaa tggaggacaa agtctccgca aaacgcagta cgtcaggatc gctgaaatgg
     1321 gctcctgtga tctgaatgcc aatgggcaat cctttgccat cccttcccgc cggcacactc
     1381 atcgccggca caccgcccag attcgctgca atggtgagga tatccgattg atacatagcg
     1441 atcgggttct ttgctttttc gccgattgcg aaggcagtcg taggagacgt tggctgaaga
     1501 atgaaatcga ggttcttaaa gaaatctata tattctgcgc gcacgagagc ttttgctttt
     1561 tgcgctttgc cgtagtatgc gtcaaagtac ccggcggata aaacaaacgt tccaagcaga
     1621 atgcgtcttt tgacttcagg accgaaccct tccgttcttg atcgaatata gatctctttg
     1681 agctcttccc ctttcgcgcg gtgtccgtat ctgattccat cgaagcgact cagattgctg
     1741 gaagcttctg cagttgcgag gatatagtag ataggaattg aatattccca gtactctgat
     1801 ttgacgggga ccatcttcgc gcccttcgaa gcaaaatagt ctacggattt ctgaaccgcg
     1861 gcgagaactt ccgcggcata gccgtccttc ttctccggca ggtaatatcc aattctgagt
     1921 ttacgaat
//
LOCUS       DAORFF010000663         1924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_49297_length_1924_cov_4.609417, whole genome shotgun sequence.
ACCESSION   DAORFF010000663 DAORFF010000000
VERSION     DAORFF010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49297_length_1924_cov_4.609417"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PKK76_18840"
     CDS             complement(<1..652)
                     /locus_tag="PKK76_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70591.1"
                     /translation="MKRFLHRLRTLAWISLFVGSVSSFFALFQEGSFSPTYLIYGLLD
                     GVIIGALIAFYVLFIVRGTFSDLARRTPFVLLLTINTAAYVLVFVLARAWAIWITGRG
                     PFDILPYDRGHFQSAAIFALIISLTINFFMQISQLLGQNTLWNFLLGKYHRPASEFRF
                     VMYLDLAGSTSIAEKLGDKQFLALLNDFFYDMTDAVLETGADIYKYVGDEAILLWTR"
     gene            740..1606
                     /locus_tag="PKK76_18845"
     CDS             740..1606
                     /locus_tag="PKK76_18845"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70592.1"
                     /translation="MIQLRIMKAPVLPILLLLVGTACTPPFSVNPSPPQNLIALGILL
                     GAPTYLTPADISNTRMWYRTETLESTYTNGSPITNWTNSISAGTYLIANVGAARPTMD
                     TSVFGGHASALFSAASSQALTFSGGTLVAPSGCTMGVVLSRNNAGGLFPIFSLIAAPS
                     TDGRMLGFNPSNQICLHDSQGAPSGLLFTGSFPASSGPSILIYTGSGTTGNFYVNGIL
                     ALSGTVASGACPVTAPTSSVAMGTKNGGGSYYDGRIAEFFYSESSITSAQAASLSCYY
                     KNKFSVTAATCL"
     gene            complement(1670..>1924)
                     /locus_tag="PKK76_18850"
     CDS             complement(1670..>1924)
                     /locus_tag="PKK76_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70593.1"
                     /translation="DLRFYDSDPSTNRLVEIDGGSTMNDGRVADPKWTGSGVAADVVQ
                     RGITAQGRRLAAGNSNYQFVARQKLPRWIYWVNAGYTASF"
BASE COUNT          464 a          501 c          469 g          490 t
ORIGIN      
        1 ttctggtcca gagtagaatc gcttcgtctc caacgtactt atagatatcc gctccggttt
       61 ccagaactgc gtctgtcata tcatagaaga aatcgtttag aagcgccagg aattgtttgt
      121 ctccgagttt ttccgcaatg ctcgtggaac cggcaagatc cagatacatg acaaagcgaa
      181 actcagatgc aggtctatga tacttaccca gaagaaaatt ccacagagtg ttttgtccta
      241 gaagctgact gatctgcatg aagaaattga tcgtaagaga aattatcaat gcaaagatcg
      301 cagcactttg aaagtgccca cggtcgtacg ggagaatatc aaatggccct cgacctgtga
      361 tccagatagc ccaggctcgc gcaagaacaa aaaccagaac gtacgctgct gtattgattg
      421 taagtaacag tacgaagggt gttcgccgtg ccagatcaga gaacgttccg cgaacgatga
      481 acagaacgta gaaagcaatg agcgccccaa tgatcacgcc atccagcagt ccgtaaatca
      541 gatacgtagg agagaagctg ccttcttgaa aaagagcgaa gaaggagctc acgcttccca
      601 cgaacaggga gatccaggcg agtgtgcgga gtctgtgcag gaatcgtttc acgctacttt
      661 ctgaaccggg agggatggcc gggaacagaa aaaaaccagg ctaagcgaca ggaattgtca
      721 ttgctccaat ttcttccgaa tgattcaatt gcgtatcatg aaggcaccgg tcctgcccat
      781 cttgcttttg ctagtcggga ctgcatgcac tccaccgttt tcagtcaatc ccagtcctcc
      841 gcagaacttg attgcccttg ggattctgct gggtgcgcct acgtatctga ctccggcaga
      901 tatttccaat actcgtatgt ggtatcgcac ggaaacgctt gaatccacgt acaccaacgg
      961 ctctcctatc accaactgga ccaattctat ctctgccggc acgtacctca ttgcaaatgt
     1021 gggagcggca cgcccaacga tggacacatc cgttttcggt ggacacgctt ctgccttgtt
     1081 cagtgcagca agctcgcaag cccttacctt ttcaggtggc acgctcgttg cgccgtccgg
     1141 atgtaccatg ggagtcgttc tttcgcgcaa caatgccggc ggtctctttc cgattttctc
     1201 tctcatcgct gctccatcca cagatgggcg aatgctcgga ttcaatccct ccaaccagat
     1261 ttgcttgcat gattctcagg gtgcgccttc gggtcttctg ttcactggat cttttcctgc
     1321 ttcttccgga ccgagcattc tgatctatac tggttctgga acgacgggga atttctacgt
     1381 gaatggtatt cttgcactga gcggtacagt tgcatcgggc gcctgtcctg tcactgctcc
     1441 cacttcctct gtggccatgg gtacgaagaa tggtggtgga agttactatg atggaagaat
     1501 cgcggagttc ttttattcag agtctagcat caccagtgct caggcggctt cactctcctg
     1561 ctattataag aacaagtttt ccgtaacggc cgcaacctgc ctttgaatga tgggacaaaa
     1621 aaagccgccc tttgcggggc ggcttctctt tgctcaagat ctttccttct tagaaggaag
     1681 ctgtgtaacc agcgtttacc cagtaaatcc agcgaggaag tttctggcgt gcaacaaact
     1741 gatagtttga gttgcctgct gccagtctgc gtccctgggc tgtgatacct ctctgaacaa
     1801 cgtcagctgc tacacctgaa ccagtccatt ttggatctgc aacgcgacca tcgttcattg
     1861 tcgagccacc gtctatttca accagacggt ttgtggatgg atcgctgtca tagaagcgaa
     1921 ggtc
//
LOCUS       DAORFF010000664         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_49573_length_1919_cov_2.494099, whole genome shotgun sequence.
ACCESSION   DAORFF010000664 DAORFF010000000
VERSION     DAORFF010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49573_length_1919_cov_2.494099"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PKK76_18855"
     CDS             <1..676
                     /locus_tag="PKK76_18855"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HNL70594.1"
                     /translation="YIVTSDAYQRQAPDPGIADKTSRIKYYQAERLDADQLMNALIRS
                     SQMQTIGDIWEQRNMIPVGDDQKMEDYDLVGVGALKTPQSAQRDFSSASEVNRPVQYE
                     TFLAIFGSGPRRDVADDEKRVSIEQVLAMMNGRLTGRIVWDFGSNKNSFIYQEYQKSH
                     DMDRVFDLMYLSVLSRHPSAEELADLKKMTQNKYVTQVDDNQNTAIMNDLIWAMINSQ
                     EFLHVR"
     gene            666..>1919
                     /locus_tag="PKK76_18860"
     CDS             666..>1919
                     /locus_tag="PKK76_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNL70595.1"
                     /translation="MSDKMKRSTFLKGAMASLGAMFVPTGKFFAEDEYENALVPSPVK
                     SVIFINMAGGMSHVDTFDPKPGLTNFSLVDSSIRGVKVTEALRNTAKELNRASLLRAT
                     WCEDGDHGMAQFLLQTSYRQPQAAGFPDIPSMGAVFSHLKSKKVEAGYFPSHVTMGER
                     GGMTGAAGFLGARNSGFHISNLDNAISYMKPAWGNFGAERLVRRGQMLDMLNDRFRKK
                     YSSAGLDLWQQMSEAALEFVNSSRLSVFDLKQERADVRARFGDNWFGKACLMAKRLAS
                     AEVPFIQITRGGWDTHDNNRGRVTEIMKDVDPALAALLGELGSSGLLKQTLVVLTSEF
                     GRTPNVGGRDGRDHWPQAWTTLIAGGNVPGGAVIGDTGEKGEKPVKDGVHWRGAAATI
                     YRAGGIDPEAMLKNEMGRPLRLVAQG"
BASE COUNT          514 a          444 c          528 g          433 t
ORIGIN      
        1 ctacattgtt acatcggatg cataccagag acaggctccg gatccaggaa ttgcggacaa
       61 gacttccaga atcaaatact atcaggcaga gagactcgat gcagatcagt tgatgaatgc
      121 attgattcga tcctctcaaa tgcagacgat aggcgacatc tgggaacaga gaaacatgat
      181 tccagtgggc gatgatcaga aaatggaaga ctatgatctg gtaggcgttg gggcactgaa
      241 gacaccgcaa agtgctcaga gggatttttc ttccgcatct gaagtgaaca gacccgttca
      301 atacgaaacg ttcctggcca tctttggctc cggcccgagg cgcgatgtag cagacgatga
      361 aaaacgcgtg agcattgaac aggtactggc catgatgaac ggtagactca caggcagaat
      421 tgtctgggac tttggctcaa acaaaaactc tttcatctac caggaatacc agaaatcgca
      481 cgatatggat cgagtgtttg atttgatgta tctttcagtt ttatctagac atccgagtgc
      541 ggaagaactg gcggacctca agaaaatgac tcaaaataaa tacgttacgc aagtggacga
      601 caatcagaac acagcgatca tgaacgattt gatctgggcc atgatcaaca gccaggagtt
      661 cctccatgtc agataagatg aagcgttcta cttttctcaa gggcgccatg gcatctctgg
      721 gtgctatgtt tgttccaact ggcaaattct tcgccgaaga tgaatacgaa aacgcgctcg
      781 tgccatctcc agtgaaatct gtgatcttca tcaacatggc aggcggcatg agccacgtgg
      841 atacgtttga tccaaagcca ggtctgacta acttttcgct cgtggattct tccattcgag
      901 gagtcaaggt aacagaagca ctgcgaaaca ctgcaaagga attgaatcgc gcgtccctct
      961 tacgcgcaac atggtgcgaa gatggggatc atggtatggc gcaatttctg ctccagactt
     1021 cttacaggca accccaggca gcggggtttc cagacattcc gtctatgggc gcggtcttct
     1081 ctcatctcaa aagcaaaaag gtcgaagcag gttatttccc gtcgcatgtc acgatgggag
     1141 aaaggggagg gatgacagga gccgcggggt ttctgggtgc gcgaaacagc ggatttcata
     1201 tttccaatct ggacaacgcc atcagttaca tgaagcccgc gtggggaaat tttggtgcgg
     1261 aacgtttggt cagacgcggg caaatgctcg acatgctcaa cgatcgcttt cgcaagaagt
     1321 attcatctgc cggtctggat ctgtggcagc aaatgtcaga agccgcgctt gaatttgtaa
     1381 attcttccag actttccgtt ttcgatctca agcaagagag agcggatgtt cgcgctcgtt
     1441 ttggtgacaa ctggtttggc aaggcatgcc tcatggcaaa gagacttgct tccgcagaag
     1501 ttccctttat tcaaatcacg cgcggaggat gggatacgca cgacaacaac cgcgggcgcg
     1561 tcacagaaat catgaaggat gtggatcctg ctctcgcagc actcctgggc gagcttggaa
     1621 gttccggtct tttgaagcag actctggttg tactgacgag cgaattcggt cgcactccga
     1681 atgtgggcgg gagagatgga agggatcact ggccgcaggc gtggactact ctgattgctg
     1741 gaggaaatgt gcctggcgga gcagtcatcg gagacactgg agaaaaagga gaaaagccag
     1801 tgaaagatgg cgtacactgg agaggagccg ctgccaccat ctaccgcgct ggtggaattg
     1861 atccggaagc aatgctcaag aatgaaatgg gccgtcccct cagactcgtt gcgcaggga
//
LOCUS       DAORFF010000665         1917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_49681_length_1917_cov_2.561224, whole genome shotgun sequence.
ACCESSION   DAORFF010000665 DAORFF010000000
VERSION     DAORFF010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49681_length_1917_cov_2.561224"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKK76_18865"
     CDS             <1..199
                     /locus_tag="PKK76_18865"
                     /inference="COORDINATES: protein motif:HMM:NF028180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70596.1"
                     /translation="AWAQEEPRNQGAWTYIDPRLRSQIPQASELSYIGRTPSPSPATG
                     YFKVHQKEQQEIMDQVLAKGE"
     gene            201..1382
                     /gene="odhB"
                     /locus_tag="PKK76_18870"
     CDS             201..1382
                     /gene="odhB"
                     /locus_tag="PKK76_18870"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNL70597.1"
                     /translation="MAASIDIKVPPMGESISEATVAGWRKKPGEQVNTGDILVELETD
                     KVTMEVPAPASGVLSKIVKNSGEIVKLGDLLATLEEGAATAAAKPATAAAAPQPAAQS
                     QNTTLAPGAARLASEAGVNPASVAGTGVRGQVRKEDIVNHLEKSTPDVPPGKSRPVST
                     AGREVVQPMSPLRKKIAERLVQAQQTAAILTTFNEVDMQRVMDLRAKYKDAFKEKHGA
                     ALGFMSFFTRAAIEALRAFPAVNAEIRGTDIVYKNYFDIGVAVGGPKGLVVPIIRNAE
                     QLSFAELEKEILRLAVKVKESSISLDDLQGGTFTISNGGVYGSMLSTPILNPPQSGIL
                     GMHNIVKRAVVIDDQIVIRPMMYLALSYDHRIIDGREAVSFLVRIKECIENPERMLLD
                     I"
     gene            1389..>1917
                     /locus_tag="PKK76_18875"
     CDS             1389..>1917
                     /locus_tag="PKK76_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNL70598.1"
                     /translation="MESFDLAVIGGGPGGYVCAIRAAQLGLKTAVIESNPTLGGTCVN
                     VGCIPSKALLDSSEKYSKALHDFKDHGIQTGSVKIDVAQMMARKDRIVKELTDGLNYL
                     MTKNKITVYRGKGSFVRTEASGTVLAIAGKEGISEITARNVVIATGSEIIRIPGFEPD
                     GETIITSDHAIALKSV"
BASE COUNT          545 a          498 c          487 g          387 t
ORIGIN      
        1 tgcctgggcc caggaagagc cgcgcaatca gggcgcgtgg acctacattg atccgcgttt
       61 gcgctctcag attccgcaag cgagtgaact atcttacatt ggccgtacgc cttcgccaag
      121 cccggccact ggctacttca aagtgcatca aaaagaacag caagaaatca tggaccaggt
      181 cctcgcaaag ggagaataga atggccgctt cgatcgacat caaggttccg cctatgggcg
      241 aatccatttc agaagccaca gtggccggct ggagaaagaa acccggagaa caggtgaaca
      301 cgggtgacat actcgtggaa ctggaaacag acaaagtaac tatggaagtg cccgcgcccg
      361 catccggcgt actttcgaag attgtaaaga atagtggcga aattgtaaag ttaggcgatc
      421 ttctggcaac tctggaagaa ggagccgcca ccgcggcggc caaaccggca acagctgcag
      481 cagcaccgca acctgcggcg caatcgcaga acaccactct tgcacctgga gcagcccgac
      541 tcgcgagtga agcaggagtc aatcctgcaa gcgttgccgg aactggagtc cgcggacagg
      601 ttagaaaaga agacatcgta aatcatttag agaaatccac tccggatgtt cctcctggaa
      661 aatcgcgccc ggtaagtacg gccggaagag aagtggttca acccatgagc cctcttcgca
      721 agaaaatcgc agagagactc gtacaggccc agcaaactgc ggccatcctg acaactttca
      781 atgaagtaga catgcagaga gtcatggatc tgcgtgcgaa atacaaagat gcgttcaaag
      841 aaaagcacgg agcagcactc ggtttcatgt ctttcttcac tcgcgcagcc attgaagctc
      901 ttcgcgcatt ccctgcggtc aacgcggaga ttcgcggaac agacattgtt tataaaaact
      961 acttcgatat tggagtcgct gtaggcggac cgaaaggtct cgtcgttccg atcattcgca
     1021 acgcagaaca actatccttc gcagaacttg aaaaagaaat cctgcgcctt gctgtgaaag
     1081 taaaagaaag ctctatctct ctggacgatc tgcagggcgg cactttcaca atttcaaatg
     1141 gcggtgttta cggctccatg ctatccactc caattctcaa tccaccgcag agtggaatcc
     1201 tcgggatgca caacatcgtg aaaagagccg tggtgataga cgatcaaatc gtaattcgac
     1261 ccatgatgta cctcgcactt tcttacgacc acagaatcat agatggccgc gaagcagtga
     1321 gcttccttgt gcgaatcaaa gaatgcattg aaaatccgga gcgcatgctc ctcgacatct
     1381 gagggcttat ggaatcattt gatctggcag tcattggcgg cgggcccggc ggttacgtgt
     1441 gcgccattcg cgcagcacag cttggactaa aaacagcagt cattgaatcc aaccctacac
     1501 tgggcggaac gtgcgtaaat gttggctgca ttccttccaa agctcttctg gattcttccg
     1561 aaaaatactc caaagcactc cacgacttca aagaccatgg aattcaaacc ggatcggtga
     1621 agatcgatgt agcgcagatg atggcgcgaa aagacagaat cgtcaaggaa ctcacggacg
     1681 gtctgaacta cctgatgaca aagaacaaaa ttactgttta tcgaggaaag ggttctttcg
     1741 tgcgaacaga ggcaagcggc accgtgcttg cgattgcagg gaaggaaggc atatctgaaa
     1801 ttacagctcg caatgtagtc atcgccacag gctcagaaat cattcgcatt ccaggattcg
     1861 aaccggacgg agaaaccatc atcacttcgg atcacgccat tgcactcaaa tcagtgc
//
LOCUS       DAORFF010000666         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_49967_length_1911_cov_2.691272, whole genome shotgun sequence.
ACCESSION   DAORFF010000666 DAORFF010000000
VERSION     DAORFF010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49967_length_1911_cov_2.691272"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PKK76_18880"
     CDS             <1..303
                     /locus_tag="PKK76_18880"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNL70599.1"
                     /translation="LRQDVPEFRAGLHYGQIVAGEMGNLRQEVAYMGDAMNTGARVLD
                     VAKKLKKTFIVSEDALSQFNVPGLEDLGEHELRGKEKRIRLYGAPDSLFEEFRRLE"
     gene            complement(306..1736)
                     /locus_tag="PKK76_18885"
     CDS             complement(306..1736)
                     /locus_tag="PKK76_18885"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020781325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNL70600.1"
                     /translation="MSDSTKKATPEKGNGKKKVEDPLKDIVSLAKRRGLVYPGSDIYG
                     GLANTFDYGPYGTELMRNLKDMWWRAFVREREDVVGLDSSILLHPEVWKASGHVDSFN
                     DPLVDCKQCRARFRADHIVEQLDPNGPKAMTLEQLVEWFEKTETQCPTCGTKKSFTTP
                     RQFNLMFETKTGSVAGKDMTVYLRPETAQGIFINFKNIVDTCRVKVPFGVAQIGKSFR
                     NEIVARQFVFRTREFEQMEMEYFCAPGTQKEWFEHWVEYCMNFLLRIGIKKENLRIRR
                     HEAEELAFYSEGTVDIEYNFPFGWGEIWGIASRTDYDLKKHAEFSGKPLEYHDSEKNE
                     KYIPYVVEPALGLNRLMLAVLCDAYYVEKEGTPEARTVLGLAPTIAPVKVGVFPLMKK
                     DGLPEIAREIYKTLSGHFQVEYDVSGNIGKRYYRQDELGTPFCITVDYDTKEDQTVTL
                     RERDSTKQERVKISDLLSILRERMAI"
BASE COUNT          470 a          500 c          437 g          504 t
ORIGIN      
        1 ctgcgccagg atgttcccga gtttcgcgcc ggcttacatt acggccaaat cgtggccgga
       61 gaaatgggaa atctcagaca agaagtagca tacatgggag acgcgatgaa cacaggcgcg
      121 cgcgttcttg acgtcgcaaa gaaactcaaa aagacgttca tcgtatctga ggatgctctt
      181 tcgcaattca atgtccccgg actcgaagac ctgggcgaac atgagctgcg aggaaaagaa
      241 aagcgcatcc gcctgtacgg tgcgcctgat tctttatttg aggaatttcg caggctggag
      301 tgaactcaga ttgccatccg ctcgcgcaga atagaaagca agtctgaaat cttcacccgt
      361 tcctgttttg tagaatctcg ctcgcgcaga gtaaccgtct gatcttcttt tgtgtcgtag
      421 tctacagtga tgcagaatgg tgtgcccaat tcatcctgac gatagtatct cttgccgata
      481 tttcctgaca cgtcgtattc cacctgaaag tgacctgaaa gcgttttata gatttcacga
      541 gcaatctctg gcagaccgtc tttcttcatg agtggaaaca cacctacttt tacaggagca
      601 atggtaggag caaggcccag cacggtacgt gcttctggcg taccttcttt ttcaacgtag
      661 tatgcatcac aaagaactgc aagcatcaga cgattgagtc cgagcgccgg ctccacaacg
      721 tatggaatgt atttttcgtt tttctctgaa tcatggtact cgagaggctt gccggaaaat
      781 tctgcatgct tttttagatc gtaatccgta cgagatgcga tcccccagat ctctccccaa
      841 ccaaaaggaa aattatattc gatgtccaca gtaccttctg agtagaaggc aagttcctct
      901 gcttcatgcc tgcgaatgcg aagattctcc ttcttaatgc caattctcaa gaggaaattc
      961 atgcaatact cgacccagtg ttcaaaccat tctttctgag ttcctggagc gcagaaatat
     1021 tccatttcca tctgttcaaa ctcgcgcgtg cgaaacacaa actgcctggc cacaatttca
     1081 ttgcgaaagc tcttcccaat ttgagccaca ccgaacggca cttttacgcg acaggtatcc
     1141 acgatattct taaaattgat gaagattccc tgggctgttt caggacgcag gtagactgtc
     1201 atatccttgc ccgctacaga gcctgtttta gtttcaaaca tcagattgaa ctgacgcgga
     1261 gtcgtgaacg atttctttgt tccgcaagtg gggcactgcg tttcagtctt ttcgaaccac
     1321 tctaccagct gttcgagagt catcgctttt ggaccatttg ggtccagctg ctctacgata
     1381 tgatctgcgc gaaagcgtgc gcgacactgc ttgcaatcaa cgagagggtc attgaacgaa
     1441 tcgacgtgac cacttgcctt ccatacttcc ggatgaagca gaatcgaact atccagaccc
     1501 acaacatctt cgcgctctcg cacaaaagct ctccaccaca tatcctttag attgcgcatg
     1561 agctctgtgc cgtacggacc atagtcaaac gtattggcca ggccaccata gatgtctgat
     1621 ccgggataga cgagtcctcg gcgttttgcc agcgatacta tgtctttcaa aggatcttct
     1681 actttcttct ttccgttgcc cttctcaggc gttgcttttt tggttgaatc agacatcaca
     1741 agctccggcg ccagatcgcc agaagcaccg agccttgcaa ctgagaatgc catttgcttg
     1801 ccgcaggacg cgcttccaga tatcgggaga tgtggagttc gattttcact ctctcgcaag
     1861 gcgatcacac caggcccgcg attcgtttcg actgggaatg attctggcag c
//
LOCUS       DAORFF010000667         1909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_50051_length_1909_cov_4.458468, whole genome shotgun sequence.
ACCESSION   DAORFF010000667 DAORFF010000000
VERSION     DAORFF010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50051_length_1909_cov_4.458468"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1909
                     /gene="ppk1"
                     /locus_tag="PKK76_18890"
     CDS             <1..>1909
                     /gene="ppk1"
                     /locus_tag="PKK76_18890"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNL70601.1"
                     /translation="TMTQTPYFNREISWLQFNFRVLEEASRDDNPLLERLKFLAISES
                     NLNEFFMVRVAGLKQVVASAVNEIQMDGKTAQETLTEIHRQVSVMVRLQYQYMGDIFA
                     GLKPSGIEFVEDFRSFTEADKEFARQFFENNVFRILTPLAIDPSHPFPLIGNNKLNMA
                     VCLKRPGFEQDFFAIVEVPTVIKRFPELPPGDGGVRRFTALEEIIKLHAPDFFPGTQV
                     TSIQGFTITRNSDIAIDEVASDNLLSTIEDELKQRRWGEAVRLSTRIGMPHHVREFLR
                     TKLDLSPEECFERPGLLNLQDLFEIANALQDRADLANNPFIPRNPVQQKKMSHIFQTI
                     RRKDILLHHPYDSFQPVLDLLDFAAMDPKTLAIKQTLYRTGGDSPLIESLIQAAENGK
                     QVTVLMELKARFDEERNIVRAREMEQHGVHVVYGLVGLKIHGKMLQIIRREDDGVKAY
                     CHLSTGNYNPNTARGYTDLALMTADREINNDVTNLFHALTGYASMPKFSRIAAAPINL
                     RETLLSLIQQEIENAEKGMASRIRLKMNALVDPDMIALLYRAARAGVRIELNIRGICC
                     LVPNAEGTEDRIRVISIVGRFLEHSRIFHFENGGDPRVFLASADWMPRNLNRRVEVMF
                     PILDPDHKHTIL"
BASE COUNT          525 a          493 c          449 g          442 t
ORIGIN      
        1 actatgacgc aaacgcctta cttcaaccgc gagatatcct ggcttcaatt caacttccgc
       61 gtgctggaag aagcgtcccg cgatgacaat cctctcctgg aaagactgaa gttcctggcc
      121 atctcagaat ccaatctaaa cgagttcttc atggtgcgag tggcaggcct caagcaggtc
      181 gttgccagtg cggtcaacga aatccagatg gatggcaaga ccgcgcaaga aactctcaca
      241 gagattcatc gccaggtgtc cgtgatggtt cgcctgcagt atcaatacat gggcgatata
      301 ttcgcaggtc tcaagccatc tggaattgaa ttcgtagaag actttcgctc atttacagaa
      361 gcagacaaag aatttgcgcg ccagtttttc gaaaacaatg tgtttcgtat tctcactcct
      421 cttgcgatcg atccttcgca tccgtttcca ctgattggaa ataacaaact caacatggca
      481 gtctgcctga agaggccagg atttgagcag gatttctttg ccatcgtaga agtgcccaca
      541 gtcatcaaaa gatttcctga acttcctcca ggggacggag gcgtgcgccg attcactgca
      601 ctggaagaaa tcatcaaact ccacgctcca gacttctttc ctgggactca ggtaaccagc
      661 atccagggat tcaccattac aagaaactca gacatagcga tcgatgaagt ggccagcgac
      721 aatctactgt ccacaattga agatgaactc aagcagagaa gatggggaga agcagtccga
      781 ttgtctacgc gcattggaat gcctcatcac gtgcgggaat ttttgcgcac gaagctggat
      841 ctttcgccgg aagaatgttt cgaaaggcct ggactactca atcttcagga tcttttcgaa
      901 atcgcaaatg ctctccagga tcgtgcagat ctggcaaaca atccgttcat tccgcgcaac
      961 cccgtgcagc aaaagaagat gagtcacatc ttccagacca ttcgcagaaa agacattcta
     1021 ctccatcacc cgtacgattc atttcaaccg gttctcgatt tgctcgattt tgcggccatg
     1081 gatccaaaga ctctggccat caagcagaca ttgtatcgca cgggtggaga cagtccactc
     1141 attgaaagcc tcatccaggc agcagagaac ggcaaacaag ttactgttct catggaactc
     1201 aaagcacgat tcgacgaaga aagaaacatc gtgcgtgcgc gcgaaatgga acagcatggc
     1261 gtccatgtgg tctacggact cgtaggtctc aagattcacg gaaaaatgct gcaaatcatt
     1321 cgcagagaag atgacggagt aaaagcgtac tgccatcttt ctactggcaa ttacaacccg
     1381 aacactgcca gaggttatac agacctggca ctgatgactg cggatcgcga aatcaacaac
     1441 gacgtaacca acctctttca cgcgctgacc ggttacgcgt ccatgccaaa gttttcaaga
     1501 atagcggccg cccccatcaa cctgcgcgaa acgcttctat ctctgatcca gcaagaaatc
     1561 gaaaacgctg aaaagggaat ggcatcgcgc attcgcctga agatgaacgc gcttgtagat
     1621 cctgacatga tcgcacttct ctatcgtgct gcaagagcag gagttcgcat tgaactgaac
     1681 atcaggggca tttgttgcct tgttcccaat gcggagggaa cagaagatag aatcagagtg
     1741 atctccattg ttggacgttt tcttgagcat tcgcgaatct ttcattttga aaatggcgga
     1801 gatccgcgcg tcttccttgc gtctgcagac tggatgccgc gcaatctgaa tcgccgcgtg
     1861 gaagtgatgt ttccgattct ggatccagat cacaaacata cgatcctgg
//
LOCUS       DAORFF010000668         1904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_50321_length_1904_cov_3.619254, whole genome shotgun sequence.
ACCESSION   DAORFF010000668 DAORFF010000000
VERSION     DAORFF010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50321_length_1904_cov_3.619254"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..690
                     /locus_tag="PKK76_18895"
     CDS             103..690
                     /locus_tag="PKK76_18895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70602.1"
                     /translation="MLRIDRTPYRLVRRFAYCVATLAVLIPCACGGPIKLEKAFLCDD
                     FAEGKCTAPPPEGGIYRFSLPAEKTKTWFDFAYHMYFHSRQTPGMRVNFSRGLNTQEE
                     STRTGAHCVYKISKGDKFAEDHMEGLRWDENGSGLWCFDYLGTMLVKFHKKYGTIQTK
                     PTPDFFPVTLNIKLEGGPVSAEQQETIYLDWTAAP"
     gene            687..1292
                     /locus_tag="PKK76_18900"
     CDS             687..1292
                     /locus_tag="PKK76_18900"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HNL70603.1"
                     /translation="MSEEHKLPEGLMEVKISDVSITNVGFAIFMKPVGSTSNKVVPIF
                     IGPLETYSISSALDGVTPPRPNTHDLIINMISELESRILHVIINDIIGNIFYARIVIQ
                     SDDGIVEIDARPSDSVAIAIRAKCPIYMHDKVYQEAGVVIGDEAKSEASENEDEETPA
                     STMTQSPLEALKTRLQKAVEQERFEEAAELRDQIERLQREN"
     gene            1237..>1904
                     /locus_tag="PKK76_18905"
     CDS             1237..>1904
                     /locus_tag="PKK76_18905"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNL70604.1"
                     /translation="MKKQQSSGTRSKGSSAKTNPALAELTSIAGVGPARAELLLAAGI
                     KSLEDLLYFFPRRYLDRTFAESTVFRAGIETTLIVTVQTAYLAHGRMSRLVVQTRTMQ
                     GEPLSLVFFRGAQYFRRVFVKDATFVVSGKLEFYGGMQMVHPDFEELDSEDAETIHVG
                     RIVPVYPGSEALKKKGLDSRGFRRILHSVLESSVTLPDLLPDGLVKSARVLDRMQSLH
                     AIHFP"
BASE COUNT          517 a          477 c          455 g          455 t
ORIGIN      
        1 tctactatta tgagtcaggc tccttcaggc cgttcgttca taccccggac ctgtatcgat
       61 acgaatctgt tatatctatt ttcaacgatt tgaggctggc tcatgttacg gatagataga
      121 actccgtatc gtctcgtgcg ccgattcgcg tactgtgtag ccactctggc agtcctgatc
      181 ccatgtgcct gcggaggtcc tatcaaatta gaaaaagcgt ttctgtgcga tgatttcgcg
      241 gaaggcaaat gcacagctcc ccctccagaa ggagggatct atcgattcag tctgccggca
      301 gaaaaaacca aaacctggtt tgacttcgca tatcatatgt attttcattc cagacagact
      361 ccgggaatgc gcgtgaactt ttcgcgcggc ttgaacaccc aggaagaaag cacgcgaacc
      421 ggagcgcact gcgtgtacaa aatctcaaag ggagacaagt ttgcagaaga tcacatggaa
      481 ggattgcgct gggatgaaaa cggttcagga ctctggtgct ttgactacct gggaaccatg
      541 cttgtgaagt ttcacaaaaa atacgggacc atccagacaa aacccactcc agatttcttc
      601 cccgttacat tgaacatcaa actggaaggc ggacctgttt ccgcagaaca acaggaaaca
      661 atctacctcg actggacggc agcgccatga gcgaagaaca caaactccct gaaggcctca
      721 tggaagtcaa aatctcagac gtgtccatca cgaacgtcgg attcgccatt ttcatgaaac
      781 cagttggatc tacatccaac aaggtggttc ccatctttat tgggcctctc gaaacgtatt
      841 ctatttccag tgctctcgat ggggtaactc cacctcgtcc gaacactcat gatctcatca
      901 tcaacatgat ttcagaattg gaatcgcgca tactgcacgt gatcatcaat gatatcatcg
      961 gaaacatttt ctatgccaga atcgtgatcc agtcagacga tgggatcgta gaaattgatg
     1021 ctcgtccttc cgattcagtg gccattgcca ttcgcgcgaa atgccccatc tatatgcacg
     1081 acaaagtgta tcaagaggct ggcgtggtga tcggtgatga agccaaaagc gaagcatccg
     1141 aaaacgaaga cgaagaaaca cccgcttcca caatgacgca atctccgcta gaggcattga
     1201 aaacaagact gcaaaaagcg gttgagcagg aacgatttga agaagcagca gagctccggg
     1261 accagatcga aaggctccag cgcgaaaact aatccagccc tggcggagct cacgtcgatt
     1321 gcaggcgttg gcccggctcg cgcggagcta ttgctggctg caggaattaa atcactcgaa
     1381 gatctactct atttctttcc acgtcgctac ctggatcgca cattcgcaga aagtaccgta
     1441 tttcgcgcgg gaatagaaac cactctgatt gtcaccgtgc aaaccgcgta tctggcacat
     1501 ggcagaatgt ccagactggt tgtgcaaacc agaaccatgc aaggagagcc tttgtctctc
     1561 gtattctttc gaggcgcgca atactttcgt cgagtgttcg tgaaagatgc aacgttcgtg
     1621 gtctcgggca aacttgaatt ttacggtggg atgcaaatgg tgcatcccga ttttgaagaa
     1681 ctggattccg aagatgcaga aaccatccac gttggaagaa tcgtacctgt ttatcctggt
     1741 tcagaagcac tcaagaagaa aggattggat agcagaggtt tcagaaggat tctacattct
     1801 gtcctggaaa gttcggtcac tctgccagat ctgctcccag atggtctggt caaatctgct
     1861 cgtgtgctcg atagaatgca atccctgcat gcgattcatt ttcc
//
LOCUS       DAORFF010000669         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_50843_length_1893_cov_3.363983, whole genome shotgun sequence.
ACCESSION   DAORFF010000669 DAORFF010000000
VERSION     DAORFF010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50843_length_1893_cov_3.363983"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PKK76_18910"
     CDS             <1..657
                     /locus_tag="PKK76_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004760800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNL70605.1"
                     /translation="TFMPKPLYGDNGNGMHTHQSVWKDGKPLFAGSGYQNLSDVAMNY
                     IGGVLKNGKSIAAFTNASTNSYKRLMPGFEAPSILAYSAQNRSASARIPFVNSDKARR
                     VEFRFPDSSANPYLAFASMLMAGIDGIKKKIDPGPPHEEDLFELSTDEIKAKGINLMP
                     YTLRDALTSLEGSSDFLRAGDVFTDHFLKTYIQYKYDTEVTPYEGRPHPIEFVNTYSC
                     "
     gene            complement(709..>1893)
                     /locus_tag="PKK76_18915"
     CDS             complement(709..>1893)
                     /locus_tag="PKK76_18915"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNL70606.1"
                     /translation="FESLLSISFGVDIVLHFFTGFRFENRIILDPRRIAVHYLRSWFI
                     LDLIAALPFFLIHGTDAAEATQLLRVIRFLQIHRLLKLLRVNEVFSQFQRKHALNPGL
                     FRLGYFVLLISLITHMLACGWILLDGITDETDPVTIYIKALYFTVTTLASVGYGDITP
                     HNNIQRIFALGLMMIGVGSYGFVIGNMASFLANRDIVRANYFKKLEEVTAFLRYRAIP
                     SDLRSQILAYYDHVWESRMGQDEAELLSDLPQSLRVDIALAMRKDLIQKVPFFREASE
                     DLLRDIVMALRPAAYIPGSFIIREGEMGDCMYIVSSGIVEIVSRDGSQVFTELTEGSF
                     LGEMALIYKTERTASARARGYCDLYVLKKRDFDFILSRYPAFASHIMKVAEERRREAA
                     SR"
BASE COUNT          530 a          494 c          425 g          444 t
ORIGIN      
        1 acatttatgc ccaagcctct gtatggtgac aatggaaacg gcatgcacac tcaccagtca
       61 gtctggaaag acggcaagcc tctcttcgca ggcagcggat accagaacct gagcgatgtg
      121 gccatgaact acatcggcgg cgtgttgaag aatggcaaga gcatcgctgc tttcaccaac
      181 gcatccacaa actcttacaa gagattgatg ccaggtttcg aagcgccttc catccttgcc
      241 tactctgcgc agaaccgcag cgcaagtgca agaatcccat tcgtgaatag cgacaaggct
      301 cgccgcgttg agttccgttt cccggattct tctgcaaacc cgtatctggc tttcgcatcg
      361 atgctgatgg ctggaattga cggcatcaaa aagaagattg atccaggtcc acctcacgaa
      421 gaagacctct ttgaactttc cacagacgag atcaaagcaa aagggatcaa tctgatgcct
      481 tacacactgc gcgatgctct cacaagcctg gaaggatctt cggatttcct gagagctgga
      541 gacgttttta cggatcactt cctaaagacg tacattcaat acaaatacga cacggaagta
      601 acaccctatg agggcagacc acatccgatc gaattcgtaa acacttactc ctgctaacac
      661 ttgagccggc ggggacttct cccgccggca ccttcatttc tttcttcact accgactggc
      721 agcttctctc cttctttctt ccgcaacttt catgatgtga ctggcgaatg ccggataacg
      781 ggatagaatg aaatcaaaat cccttttctt gagaacgtaa agatcacagt atccgcgtgc
      841 tcgcgcactt gcggttcgct ctgtcttata aatcagtgcc atttccccga gaaaactccc
      901 ttctgtaagc tctgtgaata cctgagagcc atcgcgcgat acaatctcca ctataccaga
      961 gcttacaatg tacatgcagt ctcccatttc accttcgcgg atgatgaagg atcctggaat
     1021 gtaggctgcc ggtctgagtg ccatgacaat gtcgcgaagc aaatcctcag acgcctctct
     1081 gaagaatgga accttctgaa tcaggtcctt tcgcatggcc agtgcaatgt ccactcgcag
     1141 cgactgtgga agatcgctca gcaattctgc ttcatcctga cccatcctgc tttcccagac
     1201 gtgatcatag taagcaagaa tctgagatcg cagatcagat gggatggcgc gatatctgag
     1261 gaacgcagtg acttcttcta acttcttgaa atagtttgcg cgcacaatgt ctcgatttgc
     1321 aaggaagctg gccatgttcc caatcacaaa gccgtagctt cccactccga tcatcatcaa
     1381 gcccagagcg aagatcctct gtatgttgtt gtgtggggta atgtctccgt aacccacgct
     1441 ggcaagcgtg gtcacagtaa agtagagagc tttaatgtaa atggtgactg gatctgtttc
     1501 atccgtgatt ccatcgagca agatccaacc gcatgccagc atgtgagtga tcagagaaat
     1561 caacagtaca aagtatccaa gacgaaacaa gccgggattg agtgcatgct ttctctggaa
     1621 ttgagagaac acttcattga cccgaagcaa tttcaacaga cgatgaatct gcaaaaatcg
     1681 aatcactcgc agaagctgtg ttgcttccgc cgcgtctgtt ccgtggatca agaagaacgg
     1741 aagagccgca atcaaatcca gaatgaacca ggaacgaagg taatgaaccg ctatgcgcct
     1801 ggggtccaga atgatcctat tttcaaatcg aaagcctgta aaaaaatgaa gaacaatatc
     1861 aactccaaaa gaaatcgaaa gcaaagactc aaa
//
LOCUS       DAORFF010000670         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_51004_length_1890_cov_3.893188, whole genome shotgun sequence.
ACCESSION   DAORFF010000670 DAORFF010000000
VERSION     DAORFF010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51004_length_1890_cov_3.893188"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PKK76_18920"
     CDS             complement(<1..749)
                     /locus_tag="PKK76_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNL70607.1"
                     /translation="MLDSVPPAQIPIVMSLSEQLKKIEVNARNGLAGAADLAALDAWH
                     ADYLSKKGSLTEILKGLKDLSPDEKRSTGALANTVREALESAFLARKREISQAEINER
                     LKSETYDALRPRSSHRGHLHPLTQITEELEDLFSFMGFTILDGPEVETDENNFGKLNF
                     SDDHPARDMQDTIWTAQEGVLLRTHTSSVQVRAMQILKPPFRILAPGRCFRYEETDAS
                     HENTFNQAEGMMIGENLSVANLIHVMKTFLSS"
     gene            798..989
                     /gene="rpmF"
                     /locus_tag="PKK76_18925"
     CDS             798..989
                     /gene="rpmF"
                     /locus_tag="PKK76_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNL70608.1"
                     /translation="MALPKRKTSLQKKRQRRAHHAIGKPNLVACKSCGAFIMPHRVCP
                     SCGFYKNRVVLAPKGVQKA"
     gene            complement(986..1315)
                     /locus_tag="PKK76_18930"
     CDS             complement(986..1315)
                     /locus_tag="PKK76_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70609.1"
                     /translation="MKKVLLVIFCIAFAITCNPKQPDDSSTLAILAILIATGVIKPAR
                     INTPGGTGDPGGGDGLHLLKGSCHLDQSCLVPRLMTDASVECSARGGSYSEEPCDCEA
                     TTCDWLN"
     gene            complement(1324..>1890)
                     /locus_tag="PKK76_18935"
     CDS             complement(1324..>1890)
                     /locus_tag="PKK76_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70610.1"
                     /translation="VHRLGIYYYDSFSPNPVLLYRMHDVPINAGEYIMVTLDRPNDTS
                     EDLLLGSSPTYAINTAGGPIGCGGAFPAGLAEATTYEVYSSTAGISSTDGLIFVSYDL
                     DALPMDVMCYSNRDGDMASGLMTGGMRDVFRWGTSVYNLAAFPVDTANDYDVQNSCAF
                     YTGSGSNNYVARTPTGTDTNRGSDFQQF"
BASE COUNT          472 a          488 c          469 g          461 t
ORIGIN      
        1 gaagacagaa acgttttcat cacatgaatc aaattcgcaa ccgaaaggtt ttcaccgatc
       61 atcatgcctt ctgcctgatt gaatgtgttt tcatgcgaag cgtctgtctc ttcgtatcta
      121 aagcaacgcc ctggcgccag aatgcgaaac ggcggcttta gaatctgcat ggccctcacc
      181 tgtacggaag aggtgtgagt gcgaagaagt acaccctcct gggcagtcca gatcgtatcc
      241 tgcatatcgc gcgccggatg atcgtctgag aaattcaatt tgccaaagtt gttttcatcc
      301 gtttctactt cgggcccatc gaggatggta aagcccatga aagaaaacag gtcttcgagt
      361 tcttctgtaa tctgggtgag aggatggagg tgtccgcgat gagaagatct gggtctgagc
      421 gcatcgtatg tttcactttt caggcgctca ttgatttctg cctgagaaat ttcgcgtttg
      481 cgcgccaaaa acgcagactc aagcgcctcg cggactgtat ttgccagagc gcccgtgctt
      541 cgcttttcgt caggggaaag atccttgagt ccctttagaa tctctgtgag actgcctttt
      601 ttactgagat aatccgcatg ccaggcgtcc agggctgcca gatctgcggc acccgccaga
      661 ccatttcgag cgtttacttc tattttcttg agttgttctg aaagggacat tacgattggg
      721 atttgcgcgg gcggcacgct gtcaagcatg ctcgcgaaaa aggttgcccg cacagggctc
      781 tcttgcaagt ctggttcatg gccttaccaa agcggaagac atctctccag aaaaagcgtc
      841 agcgccgggc gcaccatgca atcggtaagc caaacctggt agcctgcaag agctgcggtg
      901 cattcatcat gcctcaccgc gtttgcccgt cctgcggttt ttacaagaac cgcgtggtac
      961 ttgcacctaa aggcgtccag aaagcttaat tcaaccaatc acaggtagtt gcttcgcagt
     1021 cacacggctc ctcgctgtag gatccgccac gcgcgctaca ttctacagaa gcatctgtca
     1081 tcaagcgcgg aaccaggcaa ctctgatcca gatgacacga tcctttcaac aaatgaagac
     1141 cgtcccctcc gccaggatct cccgtcccgc ctggagtgtt gatgcgagcc ggtttgatga
     1201 cacctgtggc aatgagaatc gcgagtatgg caagagtgct cgaatcatcc ggctgctttg
     1261 gattgcaggt gattgcaaat gcgatacaaa aaataaccag cagtactttt ttcatatgaa
     1321 ttcctaaaac tgctgaaagt ccgaacctct gtttgtgtcc gttcccgtag gagtgcgagc
     1381 cacgtagtta ttcgaaccgg atcccgtgta aaacgcgcag ctattctgga catcgtagtc
     1441 attggctgtg tccactggaa acgcagctag attgtaaaca gatgttcccc accgaaatac
     1501 atcacgcatc ccaccggtca ttaggccaga tgccatatct ccgtcgcgat tcgaatagca
     1561 cataacgtcc atgggaagcg catctagatc ataggaaaca aagatcaggc cgtctgtaga
     1621 ggaaatgccg gcagtcgatg aatacacttc gtatgtggta gcctccgcga gtcccgcagg
     1681 aaatgctcct ccacatccga taggaccgcc cgcagtattg attgcgtagg ttggacttga
     1741 accaagcaac aaatcttccg aggtatcatt gggacgatcc aatgtaacca tgatgtattc
     1801 gcccgcattg atgggaacat catgcatgcg atacagtaga actggattgg gcgaaaaaga
     1861 atcataatag taaattccaa gtctgtgtac
//
LOCUS       DAORFF010000671         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_51014_length_1890_cov_3.203270, whole genome shotgun sequence.
ACCESSION   DAORFF010000671 DAORFF010000000
VERSION     DAORFF010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51014_length_1890_cov_3.203270"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /gene="rfbF"
                     /locus_tag="PKK76_18940"
     CDS             complement(<1..589)
                     /gene="rfbF"
                     /locus_tag="PKK76_18940"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HNL70611.1"
                     /translation="MLGVKVVILAGGLGTRISEESHLRPKPMIEIGEKPILWHIMKIY
                     SHYGFDDFVVCLGYKGYVIKEYFSNYFLHESDVTFDFKNQQSMMVHHHKAEPWRVTLV
                     ETGMETQTGGRVKRVAPYIDKEPFLLTYGDGVGNVDIKALVEFHKSHGRLATVTATQP
                     LGRYGALDFEGEHRVTGFREKPVGDGAWINAGFFVM"
     gene            644..>1890
                     /locus_tag="PKK76_18945"
     CDS             644..>1890
                     /locus_tag="PKK76_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70612.1"
                     /translation="MCAVQSLVSMNRSASQAIDHQLFFAGILLACMVLTSCSRSAELK
                     SYVSIPTSIPALEEFGKKNGALVQVERRQKSSGGANCGHSPVCLIVIPFVIAGALFPE
                     KYDVAIATRGQIEIYSGIFSTDGKFVQARTTEPDGIHEFRRLDLPKLHRSIIVEIAKG
                     KEEASIERVPLLPQTHLDEDYRKSIFKSGSQDDRSDLLAEAVDWMGSEADPLVKSVLD
                     DQAFGDLALARFMSYVCEDTEKISSFVRATSLDAALKSSDVTPALNALSCFPSEDPKA
                     APFLRRLAHNVCENAGRSFSYPSEAVRWGGHSSLSVSSLKKRIAGDIDGELSNCKLPA
                     ARVLWQAALDIPFTREDFETAMRDDSFRREITSHLDMNLPEERAGLFRLVQSGEDGEA
                     AIRRLESLEWTAQGQELEAIARQ"
BASE COUNT          431 a          464 c          514 g          481 t
ORIGIN      
        1 gcatcacaaa aaagccagca ttgatccacg ccccgtcacc gaccggtttt tcgcgaaagc
       61 ccgtcacgcg atgctcccct tcaaagtcaa gcgcgccgta tcttcccagt ggctgcgttg
      121 ctgtcacagt tgccagtctg ccgtgtgatt tgtgaaattc aacgagtgcc ttgatgtcta
      181 cgtttccaac tccatcgccg taagtgagga ggaatggttc tttgtctatg tacggtgcga
      241 cgcgtttcac tcgtccaccg gtttgtgttt ccattccggt ttctacgagt gtgactctcc
      301 atggttctgc tttgtgatgg tgcaccatca tgctttgctg attcttgaaa tcaaaagtaa
      361 catcagattc gtgcagaaag taattggaga agtactcctt gatgacatag cccttgtatc
      421 caaggcagac aacgaagtcg tcaaagccat agtgggagta gattttcatg atgtgccaga
      481 gaatcggttt ttcaccgatt tcgatcatgg gttttgggcg gaggtgtgac tcttcgctga
      541 tgcgagtgcc gagtccacct gcaagaatga cgaccttcac gccaagcatt ccgtaaggta
      601 gctcgtcgca ggccagcttt tttttgattg cagggggcgt ttgatgtgtg ctgttcagag
      661 cctggtttct atgaatcgct cggccagtca ggcaatcgac catcagctct tcttcgcggg
      721 gatactcctg gcatgcatgg tgcttacgtc ttgttcgcgt tcggctgaat tgaagtctta
      781 cgtatccatt ccaacaagca ttcctgcgct cgaggagttt ggaaaaaaga atggtgcgct
      841 ggttcaggtg gaaagaaggc agaagtcatc cggcggtgca aactgcggac acagcccggt
      901 ctgtctgatc gtaattccat ttgtgattgc tggagctctt ttcccggaga aatacgatgt
      961 ggcaattgcc accagaggac agattgaaat ctattccggg atcttttcga cggacgggaa
     1021 gtttgttcag gcgcgcacaa cagaaccaga cggaattcac gaatttcgca ggctggatct
     1081 tccgaaattg catcggagta tcattgtaga aatcgcaaag ggcaaagaag aggcatccat
     1141 cgagcgcgta ccgctcctgc cacagacaca cctggacgaa gactatcgaa aatccatatt
     1201 caagagcggt tcacaggacg atcgttcaga ccttcttgca gaagcggtgg actggatggg
     1261 gtccgaagcg gatccactcg tgaaatcagt gttagacgat caggctttcg gggatcttgc
     1321 tcttgcgcga ttcatgtcct atgtctgcga ggacacagaa aagatttcga gctttgtgcg
     1381 cgcaacctct ctcgatgcgg ctcttaagtc ttcagatgtc acgcctgctt tgaatgcact
     1441 ttcttgtttt ccctcggagg atccaaaggc agctccattc ttacgcagat tggcgcataa
     1501 cgtctgtgaa aacgcgggga gatcattttc ttacccgtca gaggcagtgc gatggggcgg
     1561 gcattcttcg ctttcggtgt cgagtctgaa aaagcgaatc gccggtgaca tagacggtga
     1621 gctatcaaac tgcaaattgc ccgccgccag ggttctatgg caggcggccc tcgacattcc
     1681 gtttacccgg gaggactttg aaactgccat gcgagacgat tcgtttcgtc gtgaaatcac
     1741 atcccatctg gacatgaatc ttccggagga gagggcaggg ctatttcgcc tggttcagtc
     1801 tggcgaagac ggagaagcgg cgatccggag gctggaatct ctggagtgga ccgcccaggg
     1861 ccaggaactt gaagcaattg ccagacagta
//
LOCUS       DAORFF010000672         1877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_51631_length_1877_cov_4.019210, whole genome shotgun sequence.
ACCESSION   DAORFF010000672 DAORFF010000000
VERSION     DAORFF010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51631_length_1877_cov_4.019210"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1020
                     /locus_tag="PKK76_18950"
     CDS             154..1020
                     /locus_tag="PKK76_18950"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNL70613.1"
                     /translation="MIRSTSAHKEDRLIELLDQGGNAKLRDKSGEPIISLAIRSGSDI
                     RAIESLLSHGASADEANSYGKTPLMWAAETNRVDVMKLLLAKGAHVDARAREGWSALF
                     YAAQADKVEAAEFLLSKGADFKTPDSAGRTCLMNAANVGSERLVNKFLDLGADIKART
                     ASKRNAFVWAADERQTNIMLLLLRRGTPINEADIGGMTPLYYATALPSMEVTLDLLNR
                     GANPNIASSMGRTPLMNVARMGRLDIVKLLVERGADVNMRDWFRKSAIVLARNEGHLD
                     VVQYLRSKGAVD"
     gene            complement(1032..1355)
                     /locus_tag="PKK76_18955"
     CDS             complement(1032..1355)
                     /locus_tag="PKK76_18955"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNL70614.1"
                     /translation="MKAIFAFSLALFLIQCSTNQSGGYVAPSGSVMQNTTLNRDIPAV
                     TAVGGRTGEACVTAYFNIISSGDASIKAAAAIGGISTVKSVDYRIENLLGSVYAKTCT
                     IANGD"
     gene            1345..>1877
                     /locus_tag="PKK76_18960"
     CDS             1345..>1877
                     /locus_tag="PKK76_18960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70615.1"
                     /translation="MAFKEYLTKFFLKQGDGWSFMCNQKTGPRILACALSFLCACTTT
                     RVTHENRSPASGPDPVQLYKLKVHYEDLPAAIMQRVRARALANCRFEEATKTEFLGRA
                     QYRSGPFEGNVPVGKICLWNFERELFQEGAYDRQGALAGTATHWYAERLRQYRRRIGA
                     TEISRSWYWTANGGKWTS"
BASE COUNT          514 a          465 c          503 g          395 t
ORIGIN      
        1 agagtccgtc gagaaattca gaaaaagacc ggatgcaatc ccggataaca ttcgcggcgt
       61 gctcaaaaat ccacaatgac gcgcaaaacg ttccgcatcg ttccatcgat tttccttttg
      121 tggtctctga tatcttgcag cacggaccgc gaaatgattc gctccacttc cgcgcacaaa
      181 gaagaccgac tcattgaact gctcgaccag ggcggaaatg caaaactacg cgataagagc
      241 ggagaaccca tcatcagcct ggccatacga tctggcagtg acatcagagc catcgaaagc
      301 ctgctttcgc acggagcgag cgcagatgag gcaaactcct atggaaagac acctttgatg
      361 tgggccgcag aaacgaatcg agtcgatgtg atgaagctgc tccttgcgaa aggagcacac
      421 gtcgatgcaa gagcgcgcga aggctggtct gctctgtttt acgcggcaca ggcggataag
      481 gtggaagccg cagaatttct tctttcaaaa ggcgcggatt tcaaaacgcc agattcagca
      541 ggacgcacct gtctgatgaa tgcggcaaac gtcggcagcg aaagacttgt gaataagttc
      601 ctggacctgg gtgcagatat caaagcacga accgcatcca aacgaaatgc attcgtctgg
      661 gcagcagatg aaaggcaaac aaacatcatg cttctcttgc ttcgccgggg aactccgatc
      721 aacgaagcag acattggcgg gatgactcct ctgtactacg cgaccgccct tccctccatg
      781 gaagtgactc tagatctatt gaaccgagga gcgaatccga acattgcatc tagcatggga
      841 agaactccgc tcatgaatgt agcgcgaatg ggaagactgg atatagttaa gctgctcgta
      901 gaaagaggag cagatgtgaa tatgagagac tggtttagaa aatcagcaat cgtactcgcc
      961 cgcaatgaag gtcacctgga cgtagtccag tatctgcgct ccaaaggggc ggtagattag
     1021 ttgcagaaca atcaatcgcc gttggcgatg gtgcaggtct tcgcgtacac gctaccgagc
     1081 aggttttcaa ttctgtagtc tacggacttc acagtgctga taccaccgat cgcagccgca
     1141 gccttgatgc ttgcgtctcc gctggaaata atgttgaagt atgctgtaac gcatgcttct
     1201 ccagtgcgtc cgcccacggc agttactgct ggaatgtcac gattcagagt tgtattctgc
     1261 atcacagatc cgctgggtgc aacatagcca ccggattgat tggtggagca ctggatcagg
     1321 aacagagcaa gtgaaaatgc gaaaatggct ttcaaggagt acctcacgaa attctttttg
     1381 aagcaaggtg atggctggag tttcatgtgc aatcaaaaaa ccgggcccag aattctggca
     1441 tgtgccctct cctttctgtg cgcatgcaca accacacggg tgacacatga aaatcggtct
     1501 cccgcttctg gcccggatcc agtacaactt tacaaactga aagtacacta cgaagacttg
     1561 cctgcggcga tcatgcaaag agtcagagca cgggccctgg caaattgcag gttcgaagaa
     1621 gcgacaaaga ccgaatttct cggacgagca caatacagat caggtccatt tgagggaaac
     1681 gttccggtcg ggaagatttg cctgtggaat tttgaacgcg aactcttcca ggagggtgcc
     1741 tacgaccgcc agggagcgct cgctggaact gctacgcact ggtatgcaga aagattgaga
     1801 cagtatcgac gcaggatagg agctacagag atcagccgat cctggtactg gactgcgaat
     1861 ggcgggaaat ggacttc
//
LOCUS       DAORFF010000673         1850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_53094_length_1850_cov_3.454039, whole genome shotgun sequence.
ACCESSION   DAORFF010000673 DAORFF010000000
VERSION     DAORFF010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53094_length_1850_cov_3.454039"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PKK76_18965"
     CDS             complement(<1..262)
                     /locus_tag="PKK76_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70616.1"
                     /translation="MKTSRIPAVLLLALSACAHSPGGYYFWKSEWKLEEKNLPYLNQS
                     RFAYIRMFDVALEGGEAKPSGTIRFASRWPEHLEAVPVVFITQ"
     gene            261..467
                     /locus_tag="PKK76_18970"
     CDS             261..467
                     /locus_tag="PKK76_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70617.1"
                     /translation="MAYLKFSDATGKIMADEQKDEKKNKKVNRMTLSEVDAAIKKTQE
                     HMKGMNSKYAKELVGRQAELKAKK"
     gene            complement(512..754)
                     /locus_tag="PKK76_18975"
     CDS             complement(512..754)
                     /locus_tag="PKK76_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70618.1"
                     /translation="MKKLTAATMLAVTLAVGFALGTLSAENQPHMKAALSQLGQAKDS
                     LQKATADKGGHRAKAISLVNEAIEEVKKGIAADNAR"
     gene            complement(819..1349)
                     /locus_tag="PKK76_18980"
     CDS             complement(819..1349)
                     /locus_tag="PKK76_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNL70619.1"
                     /translation="MQFCSSCGKTLSLRIPEGDNTMRHVCDSCGAIHYQNPKIVVGCL
                     PVYEGKILLCKRAIDPAYGKWTLPAGFMENGESLEAGAARETREEAEAEVRILRLHSV
                     FSIPHIHQVHFFFLAEILDGRFGAGKETLEAKLVTAEEIPWDEIAFGSVRFALKRYLD
                     APHQMETHVDSYLKPQ"
     gene            1486..>1850
                     /locus_tag="PKK76_18985"
     CDS             1486..>1850
                     /locus_tag="PKK76_18985"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HNL70620.1"
                     /translation="MKILLIRHAIAVAREEFQGSDLDRPLTDKGKKRARRAFAGLERI
                     YPGIQKIFTSPAVRAHHSARLLKRQYPEAEVHLSDLLLPEAQDDDSILSLIQSHMAET
                     IAIVGHEPNLTRLAGTMLG"
BASE COUNT          459 a          462 c          476 g          453 t
ORIGIN      
        1 cctgcgtgat gaatacaacc gggacggctt ccaggtgttc tggccatctg gacgcaaacc
       61 gaatggttcc ggagggcttt gcttccccgc cttccagggc aacatcaaac atgcgtatat
      121 aggcgaatct ggattggttc aggtagggaa ggttcttttc ttccagtttc cattccgatt
      181 tccagaagta gtagcccccc ggagagtgcg cgcaggcgct cagggctaac agtaggactg
      241 cgggaattct acttgtcttc atggcctatc tcaagttctc tgacgcgaca ggcaaaatta
      301 tggcagacga acaaaaagac gagaaaaaga acaagaaagt caacaggatg accctttcag
      361 aagtggacgc ggctattaaa aagacgcagg aacatatgaa ggggatgaat agcaaatacg
      421 ccaaagagct tgtcggtcgt caggctgaac tcaaagccaa aaagtaatct tgtaaaaagc
      481 cggggagagt ttcctctccc ctcgttcggg ctcagcgtgc gttgtccgct gcaattccct
      541 tcttcacttc ttcaatcgcc tcattgacca gagaaatcgc ttttgcgcga tgtccgccct
      601 tgtcagcagt tgccttttga agcgagtctt ttgcctgccc aagctggctc agggctgctt
      661 tcatatgtgg ctggttttct gcgctcagcg tgccgagtgc aaatccaact gccagggtaa
      721 ccgcgagcat tgtggctgca gtgagttttt tcatcgaggg actcccaaag gtttgatgaa
      781 ctgttagacg atgatttcgc acagcgggca atgaattttc actgtggttt gaggtaagaa
      841 tctacgtgcg tttccatttg atgcggggca tccaggtatc ttttgagagc aaatcgaacg
      901 gacccgaacg ctatttcatc ccacggaatc tcttctgctg tgacgagctt tgcctccagg
      961 gtttcttttc cagcgccaaa tcgaccgtct agaatttcag ccaggaagaa gaaatgaacc
     1021 tggtgaatgt gtggaatgct gaaaacagaa tgcaatctaa gaatgcgcac ctctgcttct
     1081 gcctcctcac gggtttctct ggcagcaccc gcttcgaggg attctccgtt ttccatgaag
     1141 cccgcaggta gagtccattt tccatacgca ggatcgatgg cgcgtttgca gagtaggatt
     1201 ttaccttcgt ataccgggag gcagcccaca acgatttttg gattctgata gtgaatggcg
     1261 ccgcaggagt cacatacgtg ccgcatcgta ttgtcccctt ccgggatgcg aagggacaga
     1321 gtcttgccgc aggaagagca gaattgcatg gcggcgagca tggggccaga atcggtgcag
     1381 aatcgatgcc ttcaatttct tttccgtcta cgtaaaactc agaattgtaa tacaattcgc
     1441 agaaaaacag tcccggcgaa agtctcaaat ctgagaaatg gatctatgaa gattctgctc
     1501 atacgacatg ccatcgccgt ggctcgcgag gaatttcaag ggtctgatct ggatcgccct
     1561 ctgacggaca aagggaagaa gcgcgccaga agggcgtttg caggcctgga gcggatctat
     1621 cccgggattc aaaagatttt cacttctcct gcagtcagag cacatcattc tgcaagacta
     1681 ctcaagagac agtacccgga agcagaggtt cacctctcag atttgcttct tcctgaagcg
     1741 caggacgacg attccattct atctctgatc caatctcaca tggcagaaac aattgcaatt
     1801 gtgggtcatg agcccaatct cactcgcctt gcaggcacaa tgctcggcag
//
LOCUS       DAORFF010000674         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_53314_length_1846_cov_2.968733, whole genome shotgun sequence.
ACCESSION   DAORFF010000674 DAORFF010000000
VERSION     DAORFF010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53314_length_1846_cov_2.968733"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PKK76_18990"
     CDS             complement(<1..735)
                     /locus_tag="PKK76_18990"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNL70621.1"
                     /translation="MTALDEFHQDLRRGLDRLEEKGRLRSLRVAQKGLDFASNDYLSL
                     NSSGRLLSLMQKAATDWDGVVGSTGSRLLRGHHESFEIAEEEFATFCGTESSLLFHSG
                     YAANVGVIQQFISSEDTVFCDRLCHATILDGIRMSGARRFYFHHNDLDHLRDIMRKRE
                     RKGRSWIITESIFSMDGDSPDLHSLVDLAEEEDALLYLDEAHALGVVGSTGAGLAHSL
                     GLSRRVAVLVFPCGKGPGLMGAFVCGS"
     gene            824..1750
                     /locus_tag="PKK76_18995"
     CDS             824..1750
                     /locus_tag="PKK76_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNL70622.1"
                     /translation="MAAGIFTDDLFLQHDTGRSHPESPSRLLAIRERLEGGLASHFKT
                     LSRRFADPTQIQLIHDGRYVQELERFCNAHGGYLDGDTPVSDQSYRAALLAAGAGLEA
                     ADQIQSGNISRALLLGRPPGHHSLVQRAMGFCLFNNVAICARYLQLLGHKKIAIVDWD
                     VHHGNGTQDAFYEDPSVLFISLHQYPFYPGSGAASETGRGAGAGTTLNCPLPAGSADA
                     EYKQAFEKKILPALEQFGPNAILISAGFDAHRDDPLAGMLLTATSYEWMSRKLRDFAD
                     SHCDGRLISFLEGGYNLDALADSVEAHASVLI"
BASE COUNT          470 a          524 c          444 g          408 t
ORIGIN      
        1 cgaaccacaa acaaaagcgc ccatcaaacc gggacctttg ccgcacggaa aaacaagaac
       61 ggccacgcga cgagacagcc ccagagaatg cgcaagaccc gcacccgtgg atccaacgac
      121 acccagcgca tgcgcttcat ccaaatagag cagagcatct tcttcctccg ccagatccac
      181 cagcgaatgg agatcgggac tatctccgtc catgctgaaa atagactcag tgatgatcca
      241 ggaccgacct ttcctttcgc gcttgcgcat aatatcgcga aggtgatcca gatcattgtg
      301 atgaaagtag aaccggcggg cgccggacat ccgaattcca tcgagaatgg tcgcgtgaca
      361 gagccgatcg cagaagactg tatcctctga ggaaataaat tgttggatca caccgacgtt
      421 ggctgcatac ccgctatgaa acaaaagaga cgattccgta ccgcaaaagg tcgcaaattc
      481 ttcctctgct atttcaaacg attcatgatg gccgcgcaga agacgcgaac ctgtgcttcc
      541 caccactcca tcccagtcag tcgctgcttt ttgcatcaaa gacagcaaac ggccgcttga
      601 gttcagcgaa agatagtcat tcgaagcaaa atcaagcccc ttctgggcca cgcgaagact
      661 gcgaagtcgt cccttttctt ccaatcgatc caagccacgc ctgagatcct ggtgaaattc
      721 gtccagagca gtcatcgcaa caatttcccg cagaaacagg cacaggacga ttctttttcg
      781 attcttatct tgaaatcaaa caaaacgttg gctaccatga cctatggccg ccggtatctt
      841 cacagacgat ctgttcttac aacacgacac tggccgatct catccggaaa gcccgagtcg
      901 cctccttgcg atccgtgaga gactcgaagg gggcctcgct tctcacttca aaactctatc
      961 gcggcgtttc gcagatccaa cccagatcca gttgattcat gacggcagat acgtacaaga
     1021 gctcgagaga ttctgcaatg cgcacggggg atacctggac ggagacactc cagtgtccga
     1081 tcaatcttac cgggccgcac tgctcgctgc cggcgcagga ctggaagcag ccgatcaaat
     1141 ccagtccggg aatatttcgc gagcgctctt gttgggtcgt cctcccgggc atcattctct
     1201 tgtgcagaga gctatgggat tttgcctctt caacaacgtg gccatttgcg cacgttatct
     1261 gcaattgctc gggcacaaaa aaatcgccat tgtggactgg gatgtgcatc atggaaacgg
     1321 aactcaagat gcgttttacg aagatccctc tgttttattc atcagtctcc atcaatatcc
     1381 tttctaccct ggatcaggtg cagcgtccga aacagggaga ggtgccggcg cgggtacaac
     1441 tctaaactgt cctttgcccg ccggatccgc agacgccgaa tacaaacaag cttttgaaaa
     1501 aaagatcctg cctgctcttg aacagtttgg acccaatgca atcctgatct cagcaggatt
     1561 cgatgctcac agagatgacc ctctggctgg aatgcttttg accgcaacga gctacgaatg
     1621 gatgtcgcgc aagcttcgag atttcgcaga ctcccactgc gatggtagac tgatttcttt
     1681 tctggaaggt ggatacaacc tggacgcgct ggcagattcc gtggaggctc acgcgtccgt
     1741 tcttatataa gaatctggcg aatgagaagg ccgcaggtca gaacctgcgg ccccgaaacg
     1801 ttagccggct tttacgtcga tcttcctggt tgattcctgc actttc
//
LOCUS       DAORFF010000675         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_53459_length_1843_cov_4.378076, whole genome shotgun sequence.
ACCESSION   DAORFF010000675 DAORFF010000000
VERSION     DAORFF010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53459_length_1843_cov_4.378076"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKK76_19000"
     CDS             <1..397
                     /locus_tag="PKK76_19000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70623.1"
                     /translation="YDRQGALAGTATHWYGERLRQYRRRIGATEISRSWYWTANGGKW
                     TSVLVELSIVDHAKNRRIDYVFSRDGSFLVSRRETLGEKKSGLQYNAVDSRGKSCVFV
                     DALGSEREIALPCPLPPSDVISGGESFED"
     gene            complement(394..921)
                     /locus_tag="PKK76_19005"
     CDS             complement(394..921)
                     /locus_tag="PKK76_19005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70624.1"
                     /translation="MRKIISVLLILQVLASCATVVTVKTTPVGAGVSIIYEGKERGVG
                     EATFETGSSIFADALLTFQKGGEMVTQQRMERRFSVGYFLLGYVSLFVTWLWIAPFKE
                     TQIVDLAPFLKNRPGYVAQPVLTGFTDTVTLKNGQVFAGVKAAVTADSIVMTTRDGKT
                     LVFGKADVLSIKKGN"
     gene            complement(964..>1843)
                     /gene="fliH"
                     /locus_tag="PKK76_19010"
     CDS             complement(964..>1843)
                     /gene="fliH"
                     /locus_tag="PKK76_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HNL70625.1"
                     /translation="ELEIPDKYKKFQGIEDTDEYEVDSEGNVIEQYHGPTIEEIEAEL
                     ERYRKETEEKIQKMLEEAHERARAIEEEGKSVAFNLIQESKEKAKREEETARVNAEQL
                     VERAKLEVERLIKEADMRVNEIEHEAYTKGYDAGREVGFKRGQSEVRRLIDRIGTIVG
                     QAIDVREEIIAASEKQMVDMILMVARKVIKDEVAERKEVVLNNIREALKRIKDRDRVD
                     IRVNFADLELTTAHKDELIKMMESLRKVNIYEDSRIDRGGCIIETDVGSIDARISTQL
                     KEIEEAIRNAQPMTGK"
BASE COUNT          398 a          483 c          448 g          514 t
ORIGIN      
        1 ctacgaccgc cagggagcgc tcgctggaac tgctacgcac tggtatggag aaagattgag
       61 acagtatcga cgcaggatag gagctacaga aatcagccga tcctggtact ggactgcgaa
      121 tggcgggaaa tggacttcag tgcttgtaga gctcagcatt gtggatcacg cgaagaacag
      181 aagaatcgac tatgtcttct ccagagatgg atctttcctg gtctcgagac gcgaaaccct
      241 gggcgaaaaa aaatcgggac ttcagtacaa cgcagtcgat tcgaggggta agagctgtgt
      301 attcgtggat gcgctgggaa gtgaaaggga aatagctctt ccctgtcctc ttccgccttc
      361 tgatgtcatc tcaggcggag agtctttcga ggattagttt cccttcttta tagatagaac
      421 gtcagcttta ccaaaaacga gagtctttcc gtctctcgta gtcatcacga tagaatccgc
      481 agtgactgct gccttgactc cagcgaacac ctggccattc ttcagcgtca ctgtatctgt
      541 gaatccagtg agcactggct gcgctacata gcccggacga tttttcaaga aaggagcgag
      601 atccacaatc tgcgtctctt tgaaaggagc aatccagagc caggtaacaa agagcgagac
      661 atagccaagc aggaagtagc caacagaaaa acggcgctcc attctctgct gggtgaccat
      721 ttcaccgcct ttctgaaacg taaggagagc gtccgcgaat atgctggagc cagtttcaaa
      781 agtggcttcc cctacaccgc gttcttttcc ttcgtagatg atggaaacgc cggcaccgac
      841 tggagtggtc ttcacagtga caacggtggc acaggatgcc aggacctgga gtattaataa
      901 gactgatatg attttgcgca cggtctacgg tgtagagcac cgtagaccag gacaaccatt
      961 tttctatttg cctgtcatgg gctgcgcatt gcgaatcgct tcttcgattt ccttcagctg
     1021 cgtagaaata cgagcgtcga tcgatccaac atccgtctcg atgatacatc cgccgcggtc
     1081 gatgcgggaa tcctcgtaaa tgtttacctt ccgcagtgat tccatcatct tgatgagttc
     1141 gtccttgtgc gcggttgtaa gttccaggtc cgcaaagttg acccgtatat ccacacggtc
     1201 acgatccttg atgcgcttga gagcttcacg gatgttgttg agcaccactt ctttccgttc
     1261 ggcgacttcg tctttgatca ccttgcgggc aaccatgaga atcatgtcca ccatctgctt
     1321 ttctgaggct gcaatgattt cttcacgcac gtcgatcgcc tggcccacga tggttccaat
     1381 gcggtcaatg agacgtcgaa cttcggactg gccccttttg aaaccaactt cgcgacctgc
     1441 atcgtaacct tttgtgtacg cctcgtgttc gatttcgttc acgcgcatgt ccgcttcttt
     1501 gatcagacgt tctacttcca gtttggctct ttctacgagc tgttctgcat ttacgcgcgc
     1561 cgtttcttct tcacgctttg ctttttcttt tgattcctgg atgagattga atgctacgct
     1621 cttgccttct tcttcaatgg cgcgggcgcg ttcatgcgcc tcttcgagca tcttctgaat
     1681 cttttcttcg gtttccttac gatatctttc gagctctgcc tcgatctctt cgatcgtcgg
     1741 gccgtggtat tgctcgatta cgtttccttc cgagtctacc tcgtattcgt ctgtatcttc
     1801 gattccctgg aacttcttgt atttatccgg aatctcaagc tcg
//
LOCUS       DAORFF010000676         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_53737_length_1838_cov_3.708918, whole genome shotgun sequence.
ACCESSION   DAORFF010000676 DAORFF010000000
VERSION     DAORFF010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53737_length_1838_cov_3.708918"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PKK76_19015"
     CDS             <1..420
                     /locus_tag="PKK76_19015"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNL70626.1"
                     /translation="PVFGLFLSLFAVIFLIFYRLHNPDFGPSAAWLLVFSGAVGNLID
                     KMFVKSLETGAWVFSLGPKPNHVSGVVDFVECIWFGWTPGYDMLSFSLPFFGTFRPLA
                     WLAWPVWPLFNFADSLIVVGTCMLVFSMLLTKPQPEA"
     gene            420..1001
                     /locus_tag="PKK76_19020"
     CDS             420..1001
                     /locus_tag="PKK76_19020"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNL70627.1"
                     /translation="MVEGREHESNLIRKATTGDHRAFLELIVPYQTRLLRRAQALLGS
                     MEDAEDAVQEALLMAYRSLAKFRGESGIYTYIYRIVINKCHDSVRKRRRTDSDSLDNV
                     PLISDERVSISKNHELSEESGYLIEKINSLDKKYRQVLLMRYYDDLSYQEIADLVHIS
                     VGTVKSRLFKARELLKRSIVRDGKGDEYFADMV"
     gene            985..1707
                     /locus_tag="PKK76_19025"
     CDS             985..1707
                     /locus_tag="PKK76_19025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70628.1"
                     /translation="MRTWFNNNEGELDLDSHAPADTELARKHAQHRSLDAGLKKALGS
                     LHKDARHDASKFQKRLAAALADTKPELSFGEKISLDLDRFFSILRADSYAALRYATVL
                     IIPFVGIVVYSFSQKTTPTETAAVVMESPSGSALHEEMAPDADLSPQAKDDALHKMGG
                     AYPEQPVPRAAMIAADKEFEAVAQETLQIQEDLLKKNLEMAQNTAAKKSALDQLLDFY
                     TKNGNHTAAKEIKKELTKLSAH"
BASE COUNT          455 a          464 c          460 g          459 t
ORIGIN      
        1 cctgtgttcg gtctgttttt aagtttgttt gctgttatct ttttgatatt ctatcgcctt
       61 cacaatcctg acttcggtcc gtctgcggca tggctcctcg tgttctctgg tgccgttggg
      121 aatttgatcg ataagatgtt tgtgaaatcc ctcgaaacgg gagcctgggt tttctctctc
      181 ggtccaaagc caaaccatgt aagtggtgtt gtagatttcg tggagtgcat ctggttcggg
      241 tggactcccg gatatgacat gctttcattc agccttccgt tttttgggac ctttcgtccg
      301 cttgcctggc tcgcctggcc tgtatggccg ttgttcaatt ttgcagatag tctgatcgta
      361 gttggaactt gcatgcttgt cttcagcatg cttttgacaa aaccacaacc ggaagcgtga
      421 tggtggaggg ccgcgagcac gaatcaaatc tgatccggaa agcgaccacg ggagatcacc
      481 gcgcctttct ggaattgatt gttccctacc agaccaggct tcttcgccgg gctcaggctc
      541 tgcttggcag catggaagat gcagaggacg cagtccagga agcactcctt atggcctatc
      601 gttctctggc caaattccgc ggggaaagcg ggatttatac ctacatttat cgcattgtca
      661 tcaacaaatg ccatgactcc gtgcgaaagc gcaggaggac ggactctgac tccctggaca
      721 atgttccgct catttccgat gaacgtgtga gcatttcaaa aaatcatgaa ctttcagaag
      781 aatctggcta tcttatagaa aagatcaaca gcctcgataa gaaatacagg caagtgctcc
      841 tcatgagata ctacgacgac ctgtcctatc aagagattgc agatctggta cacatcagcg
      901 ttggaaccgt gaagagccgc ctgtttaaag ccagggagct actcaaaagg tccatcgttc
      961 gagacggaaa aggagacgag tactttgcgg acatggttta acaacaacga aggcgaactg
     1021 gacctcgaca gccatgctcc ggcagatact gagcttgcgc ggaagcatgc gcaacatcgt
     1081 tctctggacg ctggcctgaa gaaggcactg ggttctttgc acaaagatgc gcgccatgat
     1141 gcaagcaagt tccagaagag actggctgcg gctctcgctg atacaaaacc tgaattatct
     1201 tttggagaaa aaatctccct ggatctggat cgcttttttt ctattctgcg cgcggacagt
     1261 tatgctgctc tgcgctatgc aaccgttctc atcattcctt ttgtgggcat cgtagtttat
     1321 tccttctctc aaaagacaac gcctacagaa acggcggccg tggtgatgga aagcccgagc
     1381 ggttctgcac tccatgaaga gatggcgccg gacgcagacc tttctccgca ggcgaaagac
     1441 gatgctcttc acaaaatggg tggtgcctat ccagagcagc cagttcctcg cgccgctatg
     1501 attgccgcgg acaaagaatt cgaagcagtc gcgcaggaaa cacttcaaat tcaggaagat
     1561 ctcttgaaga agaacctgga gatggcgcaa aacactgcgg ccaaaaaatc agctctggat
     1621 cagcttcttg atttctacac gaagaacggc aatcacactg cagcaaaaga aatcaaaaaa
     1681 gaactgacca aattgtccgc tcattgagct cacagctcaa tcgtcttgct gttgccgttg
     1741 cagaaagaag ggcggcaggc aaacgaatcc tcgatttaca ttcccccaat tccgatttcg
     1801 caatcagcac agtgccggct gacgttctgc gaacggcc
//
LOCUS       DAORFF010000677         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_54366_length_1827_cov_2.291196, whole genome shotgun sequence.
ACCESSION   DAORFF010000677 DAORFF010000000
VERSION     DAORFF010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54366_length_1827_cov_2.291196"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PKK76_19030"
     CDS             <1..549
                     /locus_tag="PKK76_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNL70629.1"
                     /translation="ESAPTFLEMLRKRSENPDDARTAARALGTLGDIRGIHELLNAWE
                     ESWSPGIVAEALKAVGPAAFEFLSERVENNPELLKRKAALSVLENWGSELTPAFQARM
                     ELTRGAQDAEKRMYVLGKLAEANEELTGAWLKEIEEQKLESSEIKRQKKRLMDEKPAT
                     PTRTTEPERLSIFSRIAKGLGK"
     gene            685..1818
                     /locus_tag="PKK76_19035"
     CDS             685..1818
                     /locus_tag="PKK76_19035"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNL70630.1"
                     /translation="MTARIFTMIVLCGCILQPACSSTVVCGDSDQKCSPLLSVLAYQR
                     RTVYNLYVLHYDSNDIGQYRVGDDGIPVYKKSYSGLPAGPGGITSDRTGRTVYVSYDT
                     SAAIQVYRIDDANGDLRLIQTVTGSGLSNSREAATVDVSNRFLYQVNGNGTISVFSID
                     LWTGMLTNTAISNTTGGGGARFGAIHPTNNYFYSGNVASNTVSFHTRASDGSLSLQGA
                     ITTAGTNGTSQIAIDSTGQYLYAPTFNAGENIYWFPIQANGSLGAVSSISCGNFCYQV
                     WIHPGNRFVFAGGTAGHFMYGRNTSTGALSGPVSTQGASFRSAAGHPSGNFLYTASTG
                     NPVSLRTLSVDSTSGAMAVIDTKSTTGNGTSACGIGLIGSIVF"
BASE COUNT          479 a          452 c          481 g          415 t
ORIGIN      
        1 gagtccgcgc ccacgttcct tgaaatgctc aggaagcggt cagaaaatcc ggacgatgcg
       61 cgcactgcgg cacgagccct gggcactctg ggagatatta gagggattca cgaactactc
      121 aatgcgtggg aggaatcgtg gtcaccaggt attgtggcag aagcactcaa agcagttggc
      181 ccggctgcat tcgaattcct ttcagagcgc gtggaaaata atccggaatt actgaagcga
      241 aaagcagcgc tcagcgtgct tgagaattgg ggatcggagc tgacgcctgc gtttcaagct
      301 cgcatggaat taaccagagg tgctcaagat gcagagaaaa ggatgtatgt tctgggcaaa
      361 ctcgcggaag cgaatgaaga actcacgggc gcgtggctaa aggaaatcga ggaacagaaa
      421 ttggaatcaa gcgaaatcaa gcggcagaag aaacgattga tggatgaaaa acctgcgacg
      481 cctaccagaa cgactgaacc agagaggtta tcaatttttt cgcgcatcgc aaaaggactg
      541 ggaaaataga tacccggctt tgcggttcct acgtagatcc ggctcatttt ctgcggcaat
      601 tacggagtgt tttgcgaaaa agcaaaagag aagcgaactt aaaagcttgc gcgcgcgaat
      661 tttcgaaatc attcattgag gtgaatgacg gcccgaatct ttaccatgat tgttttgtgc
      721 ggctgcatcc ttcagccagc atgcagctcc acagtagtct gcggcgattc cgatcagaaa
      781 tgttcgcctt tgttgagcgt gcttgcctat cagagacgca cagtttacaa tctgtacgtc
      841 ctgcactatg acagcaacga cattggccag tacagggtcg gagacgatgg aattcctgtt
      901 tataaaaaat cctactcggg attacctgca gggccaggtg gcatcacatc agatagaaca
      961 ggacggaccg tgtacgtgtc gtatgatacg tccgcagcca ttcaggtata caggatagac
     1021 gatgcaaacg gcgacctacg attgatacaa acagtcactg gatcaggcct ttccaattcg
     1081 cgcgaagcgg ccaccgtaga tgtatcgaat cgttttttgt atcaagtcaa tggaaatgga
     1141 accatatctg ttttctctat agatctgtgg actggaatgc tgacgaacac tgcgatctcg
     1201 aacactacag gcggaggcgg ggcaagattt ggagcgattc atccaacgaa taactatttc
     1261 tactctggaa acgtagcgtc gaacactgtg tcttttcaca cgcgagcttc cgatggatcc
     1321 ctctcgctcc agggagcgat caccacggcg ggcacaaacg gaacttctca gatcgcaatc
     1381 gattccaccg gtcaatatct ctacgcaccc actttcaacg caggcgaaaa catatactgg
     1441 ttccccatcc aggcaaacgg atcactcggc gcggtgtcct ccatctcctg cggaaatttt
     1501 tgctatcagg tgtggatcca tccaggcaat cgcttcgtat tcgcaggagg aacagccggg
     1561 cacttcatgt atggcagaaa cacatcgact ggggcgctga gcggtccggt gtctactcag
     1621 ggcgcatcgt ttcgatccgc cgcaggccat ccttccggaa attttcttta tacggcgagc
     1681 actggaaatc ctgtttcact gcgcactctt tccgtcgact cgacttcagg cgctatggct
     1741 gtcatcgata cgaagagcac aaccggcaat gggacctctg catgcggcat cggattgatt
     1801 ggttcgattg ttttttgaga gcaaccc
//
LOCUS       DAORFF010000678         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_55162_length_1813_cov_3.598976, whole genome shotgun sequence.
ACCESSION   DAORFF010000678 DAORFF010000000
VERSION     DAORFF010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55162_length_1813_cov_3.598976"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKK76_19040"
     CDS             <1..402
                     /locus_tag="PKK76_19040"
                     /inference="COORDINATES: protein motif:HMM:NF022443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HNL70631.1"
                     /translation="SSSSRHDAGDPMRRILYFCLSILTLLAGVWILKNPQYTLVRGTA
                     GDILVVCLMFFLFKILRDFESKKLAALLFLFACFVESLQYFHFADWLGLERGLLRTML
                     GSTFDFADIAAYLAGAVFSVLVDRVVIAYSA"
     gene            432..872
                     /locus_tag="PKK76_19045"
     CDS             432..872
                     /locus_tag="PKK76_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70632.1"
                     /translation="MRRLSGLVLLVVVLFGLEACHRARESAPVDSPVVQANPDTLPGE
                     YSGDREWSLARDRWFKETYGPCMKSAGLRMSCGGCASIYIKFVFTTNEDGRIISMEKY
                     KENVCGAPPTAELERCMTAPYQGRVFSKLRSRKFRATLGTGLSC"
     gene            938..>1813
                     /locus_tag="PKK76_19050"
     CDS             938..>1813
                     /locus_tag="PKK76_19050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL70633.1"
                     /translation="MAESENDILTRRESIVVTVDDDPAILDLIRHYLGHAGIQPVTFN
                     NAEEALQHLPDLHPDCILLDFSLPGMSGIQTCMKIREHASMRETPIIFLSADEDKKRV
                     VQALTAGANDYITKPVHEQELIVRIETHIELYLQKQRMLEQRRLLERRNEENIILNTR
                     SLMLQEVIRQFTPRSTWEKADLAAGEGMIGIADEEIDMTYFFLDIRSFTRFSDTHPAQ
                     EVIHSLNEILAPVTDIIYEEGGDVDKFIGDSIFGTFSDPLSAARAAIRSRDAIAKINA
                     NRRASGKAELGARIGM"
BASE COUNT          440 a          441 c          463 g          469 t
ORIGIN      
        1 agttcgtctt cgcgtcatga tgctggtgac ccgatgaggc gaattctata tttttgctta
       61 tccattttga ctcttctggc aggagtctgg atcttgaaga atccgcaata cactctggtc
      121 agaggcaccg caggcgatat cctcgtggtt tgtctcatgt tctttctgtt caagatcttg
      181 cgcgattttg aatcaaagaa actcgcagcg ctcttgtttc ttttcgcctg ctttgtagag
      241 agcctccagt actttcattt cgcagactgg ctcgggcttg agcgtggact gctcaggact
      301 atgcttggat ctacatttga tttcgcggac atagccgcct atctggcagg ggccgtgttc
      361 tcagtgcttg tcgatcgcgt tgtaatcgct tattctgctt gatcgtggga atcccggctt
      421 tcaatagaag aatgcgtcgt ctttcagggt tagttctact ggtcgtggtt ctatttggcc
      481 tggaagcatg ccatcgagcg cgcgaaagcg cgcctgtgga ttcgccggtt gttcaggcaa
      541 accccgacac tcttcccggt gaatattcgg gagatcgcga atggtccctg gcacgggacc
      601 ggtggttcaa agaaacgtat ggtccatgta tgaaaagcgc cggtttgcgt atgtcctgcg
      661 gcggatgtgc cagcatctac atcaagtttg tgttcactac gaatgaagac gggcgaatca
      721 tttctatgga aaagtacaaa gagaatgtat gcggggcccc tccaacggct gagctagaac
      781 gctgcatgac tgcaccttat caaggcagag tattctcaaa gctgcgctcc aggaaattca
      841 gggccaccct gggcactggc ctttcttgct gactttgact cacttcacct gacgataaaa
      901 tgctagcggc ggcgcattgc tgttccattc gtgcgccatg gcagaatctg aaaatgacat
      961 cctgactcgc cgcgaatcga ttgttgtgac tgtggatgat gatcctgcca tactggatct
     1021 gatccgacac taccttggac atgcagggat tcaaccagta acattcaaca acgcagaaga
     1081 agccttgcag catctaccag atctccatcc tgactgcatt ctgctcgact tcagtcttcc
     1141 aggtatgtcc ggaatccaga catgcatgaa gattcgtgag catgcatcta tgagagagac
     1201 tcccattatc tttttatccg cggacgaaga caagaagaga gtggtccagg cactcactgc
     1261 aggcgcaaac gattacatca caaaaccggt gcatgagcaa gaactcatcg ttcgaattga
     1321 aacgcacatt gagctgtatt tacagaagca aagaatgctg gagcaacggc gactgctgga
     1381 gcgtcgcaac gaagaaaaca tcatactcaa tacaaggtcc ttgatgttgc aagaggtcat
     1441 ccgccagttt actccacgga gcacctggga aaaagcggac cttgctgcag gagaaggaat
     1501 gatcggaatt gcagatgaag aaatcgacat gacgtatttc tttctcgata ttcgttcgtt
     1561 tacacgcttc agtgatactc atccagcgca agaggtgatc cattctttga atgaaattct
     1621 tgctccagtc acggacatca tctacgaaga aggcggagat gtggataagt ttatcggaga
     1681 ttctatcttt ggcacatttt cagatccact gtcagccgct cgcgctgcga ttcgctccag
     1741 ggatgcgatc gcgaagatca atgcgaacag acgcgcttcc ggaaaagcgg aactgggcgc
     1801 gcgaattggt atg
//
LOCUS       DAORFF010000679         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_55293_length_1811_cov_3.445330, whole genome shotgun sequence.
ACCESSION   DAORFF010000679 DAORFF010000000
VERSION     DAORFF010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55293_length_1811_cov_3.445330"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1764)
                     /locus_tag="PKK76_19055"
     CDS             complement(<1..1764)
                     /locus_tag="PKK76_19055"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNL70634.1"
                     /translation="MAAYAVKDPLVDIYQFFAAIAVLGFVGFMAMAYQMRMLDKIRGS
                     EQQLRNAIQQSHRYDFIVNTSREFMTLINREYKYEAVNDAYCEAHHKPREILIGRPVT
                     EVWGGDLFERVIRGYLDECFKGNIVNYRVGFEFPALGPRFFDVTYYPYRGAGDEITHA
                     VVVSRDHTEKKIAEDRLIHEASHDALTGLPNRHLLTDRIGHALKKKTREDDFDFALLF
                     LDLDRFKIVNDTMGHLAGDKLIIAVARVLEKCTRPGDTVGRLGGDEFVLLLENTNLQG
                     AEAVASRILRELSQPLMLGDSEVFTGASIGIAPYVSGYARSEEMLRDADTAMYRSKAL
                     GRSRYTIFDEEMRFAIKEQLTLEAEMRHGLERGEFTVYFQPIFKLTDGTLTGYETLLR
                     WKHSRRGVLGPSVFLAVAEEANLIDPLGEFVLREACRFLSRLPGKDTFVSVNVSAKQF
                     QRHGILESVIQSLADFSAEPSRLMLELKEASFARGFDEVASILRALSRLGVKLAVDNF
                     GTGNASIVNLKGLPLHMLKIDGSFARGLPHDASDRALVAGMISIAHSLGIVAGATMVE
                     TASQETLLRSLGCDTAQGFHFA"
BASE COUNT          432 a          466 c          440 g          473 t
ORIGIN      
        1 tgcgaagtga aatccctggg cagtgtcgca tccaaggctt cgcagaagtg tttcctgcga
       61 cgcagtttct accatggttg cgccggccac gattcccagg ctgtgcgcga tactgatcat
      121 tcccgctaca agtgcgcgat cacttgcatc gtgaggcaga ccgcgtgcga aggagccgtc
      181 tattttcagc atatgcaggg gcagtccctt caggttgaca atagaagcat tgcctgttcc
      241 aaaattatct acggcaagtt tcactccaag tctcgagagc gcgcgcagta tgcttgctac
      301 ttcatcgaat cctcgcgcga agcttgcttc tttgagttcg agcatgagac gcgaaggttc
      361 cgctgaaaag tccgcgagag actgaatgac tgattccagg atcccatgtc gctggaattg
      421 tttggcgctt acattcacgg aaacgaacgt atctttccct ggaagtcggg aaagaaacct
      481 gcatgcttcg cgcaatacga attctcctag aggatcaatc aagttcgctt cttctgcgac
      541 cgctagaaaa acagaaggtc caagaactcc tcttcgcgag tgtttccatc tcagtagagt
      601 ctcatacccc gtcagtgttc catccgtcag tttaaaaatg ggctggaaat ataccgtaaa
      661 ttcaccgcgt tccagcccgt gacgcatttc cgcttccagg gtgagttgtt ctttgattgc
      721 aaagcgcatt tcttcgtcga agatcgtata acgggatctt ccgagtgcct tggatctgta
      781 catcgcagtg tctgcatcgc gtagcatttc ttccgacctt gcgtaaccgg aaacgtatgg
      841 tgcaattcca atgcttgccc ccgtgaatac ttcagaatct ccgagcatga gaggctggct
      901 gagttctcgc aaaatgcgtg atgcaacggc ttccgctccc tgaagattcg tattctcaag
      961 caatagaaca aattcatctc cgcccaggcg ccccacagta tctcctggtc ttgtgcattt
     1021 ctcaagaacg cgcgccacag caatgatcag tttgtctcct gccagatgtc ccatggtgtc
     1081 gtttactatc ttgaagcgat ccaggtccag aaacagaagt gcgaaatcaa aatcatcttc
     1141 gcgtgttttc ttctttagcg cgtgtccaat tctatctgtg agcaaatgcc gattgggcaa
     1201 tcctgtcagc gcatcgtgtg acgcttcgtg aatcagtctg tcttctgcta ttttcttttc
     1261 agtatggtct ctggatacga cgactgcatg agtgatttca tcgcctgcgc cgcgataggg
     1321 atagtaagtt acatcaaaga agcgcgggcc gagagcagga aattcaaatc caactctata
     1381 atttacaatg ttgcctttga agcattcgtc cagataaccg cggatcactc tttcgaatag
     1441 atctccgccc cagacttctg ttaccggtct gccgatgagt atttcgcgtg gtttgtgatg
     1501 agcttcgcag taggcatcat tgacagcctc atacttgtac tcgcgattga tgagagtcat
     1561 gaattcgcgg gacgtgttta caatgaagtc gtaacggtga gattgctgaa tggcgttgcg
     1621 cagttgttgt tcgcttccgc gaattttatc cagcatgcgc atctggtaag ccatggccat
     1681 aaacccaaca aatccgagca ccgcgatggc agcaaaaaac tgatagatgt caacgagcgg
     1741 gtctttgact gcataggctg ccacagctgc gcccgcagtc gaaaacagta aaaaagaaag
     1801 gagcccggcg c
//
LOCUS       DAORFF010000680         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_56272_length_1795_cov_4.405747, whole genome shotgun sequence.
ACCESSION   DAORFF010000680 DAORFF010000000
VERSION     DAORFF010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56272_length_1795_cov_4.405747"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKK76_19060"
     CDS             complement(<1..242)
                     /locus_tag="PKK76_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility ABC transporter"
                     /protein_id="HNL70635.1"
                     /translation="MKSFFSALNHNTLFIRVNTVLLFLLLNGAASSWNCRWDMSRDRV
                     NSVTESTEKVFARQRDPVLVEAYITSDLPGELKAML"
     gene            complement(239..970)
                     /locus_tag="PKK76_19065"
     CDS             complement(239..970)
                     /locus_tag="PKK76_19065"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNL70636.1"
                     /translation="MKFLFRRELSNYFNTPIGYVFAVACLFFNFVFFFAGIWAIPDLF
                     PAFWDARSASIRSYMNLLPLTFILMVPAVSMRIWAEERKSGTLELISTLPLREVDLVI
                     AKFLGAWVYVGLLIVASLPLAITIWFMGHLDWGSTLALYAGSILMAGAYVSTGMVVSA
                     LTREQIVAFIITFFISLYMFISNYYIVSQHLSPGAARLIGFFSHSHHFTTFSRGLLDA
                     ADVMYYVTFIGMMLTANVWILRRER"
     gene            complement(967..>1795)
                     /locus_tag="PKK76_19070"
     CDS             complement(967..>1795)
                     /locus_tag="PKK76_19070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNL70637.1"
                     /translation="ITGLLGPNGAGKTTTMRMITGYLRPSSGDILIDGESVRDEENLT
                     GIKRRIGYLPETTPLYPEMLVSEFLKFMGEVRGVHGAELDASAVQMVDRLDLGSHLYS
                     PIGILSKGFRQRVALAGTLIHKPEVIILDEPTSGLDPNQISEIRSLIVELGKTATVIL
                     STHILQEVEELCNRVIIINRGRVVADETTAVLKSVQACSVVARGEDVEKKLASIPIVT
                     SVIAGQNHKDGFRQYLCEVGENGPEKLFAELAGKSLEVREFAPVARSLQSVFEELTN"
BASE COUNT          489 a          448 c          439 g          419 t
ORIGIN      
        1 ctgagcattg ccttgagttc accaggcaaa tcagaggtga tgtatgcttc aacaagtacg
       61 ggatcgcgct gccgtgcgaa gactttctcc gtgctttctg tcacagaatt cactcgatcg
      121 cgactcatgt cccatctgca attccaggaa ctggcggctc cattcaaaag caaaaacaaa
      181 agcacagtat tgacgcgaat gaacaatgtg ttgtgattga gagcggaaaa aaaagatttc
      241 atctctccct cctgagaatc cacacattcg ccgtgagcat catgcctata aatgttacgt
      301 aatacataac atcagccgca tccagcaggc cacgagaaaa agttgtaaag tgatggctgt
      361 gagagaaaaa cccgatgagt cttgctgcac cagggctgag atgctgagat acaatgtagt
      421 agttggaaat gaacatgtat agactgataa agaatgtaat gatgaaagca acgatctgtt
      481 cacgcgtgag agcggacaca accattccgg tgcttacgta tgcgcccgcc atgagaatgg
      541 agccggcata gagtgccaga gtgctacccc agtccagatg acccataaac cagatggtaa
      601 tggcaagcgg aagagaagcg acgatcaaca gtccaacgta gacccaggct cccaggaatt
      661 tcgcgatgac cagatccact tcccggagag gaagagtgga gatcaactct agagttcctg
      721 attttctttc ctctgcccag atgcgcatgc tcactgccgg aaccatgagt ataaatgtga
      781 gcggaagcag attcatgtac gatcggatgg aagcgctgcg tgcgtcccag aatgcaggaa
      841 acaaatccgg aatcgcccag attcctgcga agaaaaatac aaagttaaaa aacaggcaag
      901 cgaccgcaaa cacgtagccg atgggagtgt tgaagtagtt gctcagttca cgcctgaata
      961 agaatttcaa tttgtcagct cctcaaagac ggactgaagc gatctggcca caggcgcaaa
     1021 ctctctgact tccagagact tgcccgccag ctctgcaaag agtttctccg gaccgttctc
     1081 ccccacttcg cacaaatatt gacgaaagcc atctttgtga ttttgtcccg cgattacaga
     1141 agtcacaatt ggaatggatg caagcttctt ctccacatct tctccgcgtg ctacaacaga
     1201 acaggcctga acgcttttga gaactgctgt ggtttcgtct gctaccactc tgcctcgatt
     1261 gatgatgatc acgcgattgc agagttcctc tacttcctga aggatgtgag tggaaagaat
     1321 cacggtggcc gtttttccaa gttcaacgat caaagaccgg atctctgaaa tctggtttgg
     1381 atcgagaccc gatgttggtt catccaggat gatcacttcc ggtttgtgaa tcagggttcc
     1441 cgcaagtgcc actctctgac gaaacccttt cgatagaatg ccaatgggcg aatagagatg
     1501 acttcccaga tcgagccgat ctaccatctg gaccgcggaa gcgtctagct ctgctccgtg
     1561 cacgccgcga acttccccca tgaatttcaa aaactcagat acaagcatct ccggatagag
     1621 aggagttgtt tccggaaggt aaccgattcg ccttttgatt ccggttagat tttcttcatc
     1681 gcgcactgat tcgccgtcga tgaggatatc gccgctgctg gggcgaagat aacctgtaat
     1741 catgcgcatg gtggtcgttt tacctgcgcc gtttggtcca aggagtcccg tgatg
//
LOCUS       DAORFF010000681         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_56411_length_1793_cov_3.019563, whole genome shotgun sequence.
ACCESSION   DAORFF010000681 DAORFF010000000
VERSION     DAORFF010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56411_length_1793_cov_3.019563"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..746)
                     /locus_tag="PKK76_19075"
     CDS             complement(3..746)
                     /locus_tag="PKK76_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70638.1"
                     /translation="MSVEKSVKEIRATGLLVVFAALCTALSAGIITAVAGSPKPNPGS
                     AFQNAIVEFELAVNSREVQAVLSQPTEELARARRTQFNYLNYADYLFMVCYSALNATI
                     FLYVAALNRSRFRERFVGNAFIRFGLLLSFLMLAGDALENVQLLRLTSMPEPEMSSTI
                     KALIIFTRIKWGALFTAAILLGLAYASYSGRTGLLFVGFLYATAGSIGIMSLIVLSGR
                     PLCEWGTLLLAVAWLVSAVHAGVRTFRRA"
     gene            798..1601
                     /locus_tag="PKK76_19080"
     CDS             798..1601
                     /locus_tag="PKK76_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70639.1"
                     /translation="MKFGELKEDFRKRWPEWSARIIQTVRELPSTIRQAAKDSWPVIQ
                     SEARALPARLKAVPGHVVGFFAKLASVDGGPLRKLTFLWCFALGFSAVCILGRTNPFA
                     LLIPVWGVTRPASDSRSAVSLRMFPAEGDAIISVTRKLRLNDSLEHNLEALARTVSET
                     AGIHDTSAPVANLPDYAFAIRKVWVTKEKSCILDFRSETLRVETEQYTRNRTKIGEKA
                     VLLDRYFQSLTESIFASAIGCKSVRYLLDGKVDQVKEMKFDLKVERTPG"
     gene            complement(1603..>1793)
                     /locus_tag="PKK76_19085"
     CDS             complement(1603..>1793)
                     /locus_tag="PKK76_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70640.1"
                     /translation="METMIFPADRFLVRLGVTGHRFYASGTVKAHGLDLLYNVLPPQQ
                     MFLNTKEWGAKISLEYLL"
BASE COUNT          472 a          478 c          453 g          390 t
ORIGIN      
        1 tactaagcgc ggcgaaacgt tctgacacct gcgtgcactg cggacaccag ccaggccacg
       61 gcaagcagca aggttcccca ttcgcatagc ggccttccgc tcaaaacaat cagggacata
      121 atgccgatgg acccggcggt cgcgtaaaga aaccctacga aaagcaaacc cgtccttccg
      181 gagtaagaag catacgcgag gcccaacaag atcgcggcag taaatagggc gccccacttg
      241 atgcgcgtga aaatgatcaa tgccttgatc gtggaactca tctcaggttc gggcatagaa
      301 gtgagtctta gaagttgaac gttctcaagc gcatcacctg cgagcatcag gaaagacaaa
      361 agcagtccaa atcggatgaa tgcgtttcct acaaaccgct ctctgaaacg cgaacgattg
      421 agcgccgcca catacagaaa gatcgtggca ttcagagcac tgtagcatac catgaacaga
      481 tagtctgcgt aatttaggta gttgaactga gttcgtctgg ctcgtgccag ttcctcagtc
      541 ggctggctaa gaacggcctg cacttcccgc gaattcacag ccagctcaaa ctcaacgatg
      601 gcattctgga atgcgcttcc aggatttggc tttggcgatc cagccacggc ggtgatgatc
      661 ccggcagaaa gggcagtgca cagggctgcg aagaccacca ggagcccggt agcgcggatt
      721 tctttgacag acttttcgac ggacatgaga cgcaatttgg tcgacgcaca aatgcggaca
      781 ataaagttta caagagcatg aagtttggcg aactcaagga agatttccgc aaacgctggc
      841 cagaatggtc cgcaagaatc attcagacgg tccgtgaatt gccctctacc attcgccagg
      901 cggcgaagga ttcgtggcct gttattcaat cggaggcgcg agccctccct gccaggctca
      961 aagccgttcc cggtcacgtg gtgggtttct ttgctaaact ggcctccgta gatggcgggc
     1021 cattgcgcaa gctaaccttt ctctggtgct ttgccctggg tttcagcgca gtctgcattc
     1081 tcgggcgcac aaatcctttc gctcttctaa ttccagtctg gggagtcacc aggcctgctt
     1141 ccgactcccg cagtgcggtt tcactgcgca tgtttccagc agaaggggat gccatcattt
     1201 cggttacgcg gaagcttcgc ctgaacgatt cgctggaaca caatctagaa gcactcgcac
     1261 gaacagtttc ggaaacggca ggaattcacg acacttctgc tccggttgcc aaccttcccg
     1321 attacgcatt cgcgatcagg aaagtatggg tcacgaaaga gaaaagttgc attctggact
     1381 ttcgttcgga aactcttcgc gtagaaacag aacagtacac tcgcaatcgt acaaagatag
     1441 gcgaaaaggc agtattgctg gaccgttatt ttcaatcgct cacggaatct atcttcgcat
     1501 ccgcaatcgg atgcaaaagt gtgcgctatc ttttggatgg caaagtagac caggtcaaag
     1561 aaatgaaatt tgatttgaaa gtggaaagaa caccgggctg atctaaagca gatattccaa
     1621 agagatcttc gcaccccatt ccttcgtgtt caaaaacatc tgctgcggtg gcagcacatt
     1681 gtacagcaaa tcaagtccat gcgccttcac ggttccactt gcatagaacc tatgcccggt
     1741 cacacccaat cgcacaagaa aacggtcagc gggaaaaatc attgtctcca ttt
//
LOCUS       DAORFF010000682         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_56588_length_1790_cov_4.063401, whole genome shotgun sequence.
ACCESSION   DAORFF010000682 DAORFF010000000
VERSION     DAORFF010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56588_length_1790_cov_4.063401"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1269)
                     /locus_tag="PKK76_19090"
     CDS             complement(91..1269)
                     /locus_tag="PKK76_19090"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNL70641.1"
                     /translation="MQDLFVSLGAGVHQIPLIEAARAMGYMVVAVDKNLDAPGFEYAH
                     ERIQRSILRPASIRRKLEAIAGDPVAIVARSYGKALISSAALSDHLGLQGPGVQAVRF
                     FQNKSAYKSILEEAGILVPRRFQASQIARIKKDMRLIARPVAGHGKLGIEILETRKDR
                     IRFAGRQEEGWLIEELVSGTEVTVLGASHRGSYRTLLLTSKRISQSPPHFAEIMHRYP
                     ALISSQTKEKIDQICAKIVEVSGLRTSPLVSEFIVTEDESIYLVESAPETGGEFLADF
                     AFSTATGTDYFKWFIRLLTEDSMPELNLADRAAVIRYLMPAEGQLIGLEFPKWLKSCK
                     EFAFEKKLIAPGTRTDLKHGNLHRLAAIGLRGGTSEIETLITLADKAAQETNIEYKSA
                     "
     gene            1349..>1790
                     /locus_tag="PKK76_19095"
     CDS             1349..>1790
                     /locus_tag="PKK76_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HNL70642.1"
                     /translation="MLRGLYTGSSGMIMNQYKLDVISNNLANVDKTGFKADEAIFKSF
                     PEMVLHRTREDGLGYTPAGSFDLAPTVGKLGLGSEFNESFTRFEQGAAKQTGNPMDFM
                     IDDRGNERPSFFVVRTDKGDRLTRGGSFILDREGFLVNPDGHPLM"
BASE COUNT          399 a          439 c          478 g          474 t
ORIGIN      
        1 ggataatcct gaacggatct gcttctgccg tagtgtctat cccaggcgct gcgattggga
       61 agcacatccg gaacggaaat gggtggcacg ttaagcgctt ttgtattcaa tgtttgtttc
      121 ctgtgcggct ttgtccgcga gtgtgatgag ggtttcgatt tcagatgttc ctcccctgag
      181 cccgatggcg gccagtctat gaagattgcc atgtttgaga tctgttctcg tgccgggtgc
      241 tatgagcttt ttttcaaagg caaactcttt gcatgacttg agccatttgg ggaattcaag
      301 tccaatgagt tgtccttctg ccggcatcag gtagcgaatg accgccgccc gatctgctag
      361 atttagttca ggcatggagt cttctgtcaa aagtcgaatg aaccatttga aatagtccgt
      421 gcctgtggct gtgctgaatg caaaatcggc cagaaattcg ccgcctgttt cgggtgccga
      481 ttctaccaga tagatgcttt catcttccgt tacaatgaat tcgcttacga gaggagaggt
      541 gcgaaggccg gaaacttcca caatcttcgc acaaatctga tcgatcttct cttttgtctg
      601 agatgagatc aatgccggat aacggtgcat gatttctgca aaatggggag gggattgaga
      661 tatccgtttt gaagtgagaa gtagagtacg gtagctgccg cgatgacttg caccgagtac
      721 agtaacttcc gttccgctta cgagctcttc aatcaaccag ccttcttcct gcctgcctgc
      781 gaatctgatt ctatctttgc gtgtttccag gatttcgatt ccgagtttgc cgtgtcctgc
      841 gaccggccgc gcaatgagtc gcatgtcttt cttgatgcgc gcgatctgcg aagcctggaa
      901 tcttctcgga accagaatgc ctgcctcttc tagaatggat ttatacgcgc tcttgttctg
      961 aaagaatcgg actgcttgaa ctccgggccc ctggaggccc aggtgatcgg acagagccgc
     1021 gctggaaatg agcgcctttc cataggatcg agcaacgatg gccacaggat ctccggcaat
     1081 cgcctcgagc tttctgcgaa tcgaggccgg tcgaaggatg cttctctgga tcctctcatg
     1141 agcatactcg aacccgggcg catccaggtt tttgtctacc gccacgacca tgtagcccat
     1201 ggctctggcg gcctcaatca gcgggatctg gtgaacgccc gcgcccagag atacaaataa
     1261 gtcctgcatc taattaagct tcggcgcgga gacataaaaa tgtaggcaaa atgattcaga
     1321 tcgggcgtag aatgccgatt tcttagctat gcttcgcgga ctttacaccg gttcatccgg
     1381 tatgatcatg aaccagtaca agctggatgt catttcaaac aatctggcaa acgtggataa
     1441 gacaggcttc aaggcggatg aagcgatctt caaatcgttt cccgagatgg tgctccatcg
     1501 cacacgcgaa gacggtctcg gatatactcc ggcgggcagt ttcgatctgg ctccaacggt
     1561 tggcaaactg ggtcttggtt ctgaattcaa cgaaagcttc acgcgattcg agcagggtgc
     1621 agccaaacag acaggcaacc ctatggattt catgattgat gatcgtggca atgagagacc
     1681 ttccttcttt gtggttcgca cggataaagg cgatcggctg actcgcggtg gttcttttat
     1741 tctggatcga gaaggcttcc tggtaaatcc ggatgggcat cctttgatgg
//
LOCUS       DAORFF010000683         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_56714_length_1788_cov_3.118292, whole genome shotgun sequence.
ACCESSION   DAORFF010000683 DAORFF010000000
VERSION     DAORFF010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56714_length_1788_cov_3.118292"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PKK76_19100"
     CDS             <1..331
                     /locus_tag="PKK76_19100"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNL70643.1"
                     /translation="ETAYFLRPMVILYRTSWINVILASLLMRTRMIGIVNILARRQVA
                     LELLQTEVTPENIAREIVRIFEDDGYRRAMVSELEYVKRSLGSGNPAEKAARAIQTFA
                     RSTHSAR"
     gene            345..1256
                     /gene="argF"
                     /locus_tag="PKK76_19105"
     CDS             345..1256
                     /gene="argF"
                     /locus_tag="PKK76_19105"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNL70644.1"
                     /translation="MRHLLSPTDLSEKEFFRILDRAREHRNNRALAKDAVAGKSIALL
                     FEKSSTRTRLSFSVAVTEMGGTPISLEAMQLQLGRGESLEDTTEVLARYCHAMMVRAR
                     DHRNLEVMAGMNRIPIINGLSDLHHPCQIIADYMTLEQLGFSLHGGLRIAFVGEPNNV
                     FNSLAVAASYAKARITIASPRAYSQIHPAIQQELTRLKTEFQQFTEPAGAVRDTQVVY
                     TDTWVSMGQEAEEETRRKDFADYCVTTELLSLAASGHVVLHCLPAHRGEEITAEVMEK
                     YKHSIFEQAENRLHAQKAILEWIFGIL"
     gene            complement(1253..>1788)
                     /locus_tag="PKK76_19110"
     CDS             complement(1253..>1788)
                     /locus_tag="PKK76_19110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNL70645.1"
                     /translation="KTNFWIGIAPFAGHAQKMWPLDKMRELVGLLSKLDATVFLFGGK
                     GKEKEILESIHAEHPEKTHVVAGNLTLEGEMALILRLDVMIAMDSFNMHIASLLGTST
                     ISIWGSTHPFSGFGPYGPNSVIVQIDPSVLPCRPCSIFGNKPCYRKDLACMNWISAER
                     VFEETTKLLDIGPAQTV"
BASE COUNT          465 a          464 c          443 g          416 t
ORIGIN      
        1 cgaaacagcc tacttcctca ggcccatggt gattttgtat cgcacgagct ggatcaacgt
       61 aattctcgcc tctctcttga tgcgcactcg catgatagga attgtaaata tcctcgcgcg
      121 caggcaggtt gccctcgaat tgctccagac ggaagtgacc cccgaaaaca ttgctcgcga
      181 aatcgtccgg attttcgaag acgacggcta tcgccgcgcc atggtttctg agctggaata
      241 cgtaaaacga tcgctgggaa gtggaaatcc tgcagaaaaa gcggccaggg ccatccagac
      301 atttgcaaga tccacccatt cggcccgata atcaaaggac aagtatgaga cacttattgt
      361 cgccaaccga tctttcggaa aaagaatttt tcaggattct ggaccgcgca cgcgagcaca
      421 gaaacaatcg cgcgcttgca aaggacgcag ttgcgggaaa gagcattgca ctgttgtttg
      481 aaaagtcatc cacgcgcacc cgtttgagtt tcagtgttgc agtcactgag atgggtggaa
      541 cgcccatttc tctggaagcc atgcagctac aactcggccg cggtgaaagc ctggaggaca
      601 caacggaagt cctcgcacgc tactgtcacg ctatgatggt tcgtgctcga gatcatagaa
      661 acctggaagt gatggccggg atgaatcgca ttccgatcat caacggtctg tccgatcttc
      721 accacccctg ccagattata gctgactata tgaccctcga gcaattgggc ttcagcctgc
      781 acggtggtct tcgcattgcg tttgtcgggg agccaaataa cgttttcaat tctctggcag
      841 tggcggcttc gtatgcgaaa gcgcgcatta cgatcgcctc acctcgcgct tacagtcaga
      901 ttcaccccgc aatacagcag gaactcacaa ggctcaagac tgagtttcaa cagttcacgg
      961 aaccggccgg tgcagtacgc gatacgcagg tggtctatac tgatacctgg gtttccatgg
     1021 gccaggaagc agaagaagag acgcgaagaa aggactttgc agactattgc gtcaccactg
     1081 aacttctatc tctcgcagcc agtggacatg tagtgcttca ttgtctgcca gctcatcgcg
     1141 gagaagaaat cacagcagaa gtcatggaaa aatacaaaca ttccatcttc gaacaggctg
     1201 agaacaggtt acacgcacaa aaagcgattc tggaatggat ctttggaatt ctctagactg
     1261 tttgtgcggg tccgatgtcc agtagctttg tagtctcttc gaacactctc tctgcggaaa
     1321 tccagttcat gcacgccaga tctttgcgat agcatggctt atttccaaag atagaacacg
     1381 gcctgcacgg caacacggat ggatcaatct gaacgatcac cgaattaggt ccgtagggac
     1441 caaatccaga aaaaggatga gtggatcccc agatcgaaat ggtgctggtt ccgagaagag
     1501 aagcgatgtg catgttgaaa gagtccatcg caatcatcac atccagacgc agaatgagcg
     1561 ccatttcccc ttccagtgtg agatttccgg ccactacatg agtcttctca ggatgctctg
     1621 cgtgaatgga ttctagtatt tccttttctt ttccttttcc gccaaacaaa aacactgtgg
     1681 catcgagctt cgaaaggaga cctacaagct cccgcatctt gtccagaggc cacattttct
     1741 gcgcatgtcc cgcgaatggc gcaatgccga tccagaaatt ggttttgt
//
LOCUS       DAORFF010000684         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_57308_length_1778_cov_3.060940, whole genome shotgun sequence.
ACCESSION   DAORFF010000684 DAORFF010000000
VERSION     DAORFF010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57308_length_1778_cov_3.060940"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="PKK76_19115"
     CDS             <1..1024
                     /locus_tag="PKK76_19115"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HNL70646.1"
                     /translation="DDHTVDILCKLTEKSAVEDSFALSRLEAAAKRLPGRLQLSSARR
                     QTRVVEGCTVIKNERGLAPGMIVEIGTNGPFIAAMPGVPSEMRGMFDGTLFPYIKSRF
                     VSHSRERLSFFIYGHGESQVQARLFGDKETQRSIPETLPDDFIWGITAQPGFSKVFFE
                     TTDKSFLGLIDQAAENIFGNAKLDATVEDKLHDYCIENKIKVALAESCTGGLISRILT
                     DRPGASAYFQGGAVVYANAAKEQILGVSSAILETHGAVSEECAMAMAEGARRIYSADA
                     SVAITGIAGPEGGTAAKPVGTVHIAASYKDKSMHAKLFLPFDRDRIREYSARVAILHL
                     FNLLRS"
     gene            complement(1037..>1778)
                     /locus_tag="PKK76_19120"
     CDS             complement(1037..>1778)
                     /locus_tag="PKK76_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70647.1"
                     /translation="PSQQPEGVERMARDVGQVRGLRRVIINVSPFFLFKSEVLQSFLS
                     YYRNTYDWSFADLNPDLMTLSGKNTGDSVYRMIRFLPVLRLRDRMSPALASADPISFV
                     AGRKETNLKIEKILNDQRGFWVWKSTDQTCNADIPPPPPMLLAYADRPGAVESLERSV
                     QGLRARGLEVFLVYIPLSDVWASLALRGTDDRVIAVMRRMEKVGSVTLPIADRAQYKG
                     LFRDWTHLNRCGAEKFTSWLFSQSLLQN"
BASE COUNT          483 a          464 c          431 g          400 t
ORIGIN      
        1 agatgatcac actgtagaca tactctgcaa gctcactgaa aaatcagccg tcgaagattc
       61 gtttgctctt tccagactcg aagccgcggc caaacgcctt ccggggcgac tgcaactttc
      121 ttccgcgcga agacaaacgc gagttgtaga aggatgcact gtcatcaaga acgaaagggg
      181 actcgctccc ggaatgatcg tggaaatagg aacaaacgga ccattcattg cggctatgcc
      241 aggagtccct tccgaaatgc gcggcatgtt cgatggaact ctctttcctt atatcaagtc
      301 cagattcgtt tctcacagtc gcgaaaggtt gagtttcttt atctacggtc acggagaatc
      361 ccaggtgcag gcgcgtctct ttggagacaa agagacacag aggtccatac cagaaacgct
      421 tcccgacgat ttcatctggg gcatcacggc gcaacccggt ttctcgaaag tatttttcga
      481 aaccacagac aaatcgtttc tgggtttgat cgatcaagca gcagagaata ttttcggaaa
      541 tgcgaaactc gatgcaaccg ttgaagacaa gttgcacgat tactgcatcg aaaacaaaat
      601 caaggtggcg cttgcagagt cctgcactgg cggcttgatt tccaggatcc tcacagacag
      661 accaggagca tcggcttatt ttcagggcgg agcagtcgta tacgcaaatg cggccaaaga
      721 gcaaattcta ggtgtatcat ccgccatttt agaaacgcac ggagctgtaa gcgaagaatg
      781 cgccatggcg atggcagagg gggcaaggag aatctattcg gcagacgcgt ccgttgcgat
      841 cacaggaatc gcgggcccgg aaggtggaac tgccgcaaag ccggttggca cagtacacat
      901 tgccgcctcc tacaaagata agtccatgca tgcgaaactg tttttgccct ttgaccggga
      961 ccgcatccga gagtattcgg cgcgagttgc catcttacac cttttcaatt tgctcaggtc
     1021 ctgagcgctt ccagaatcaa ttctgcaaca gtgattgcga aaaaagccag gaagtaaatt
     1081 tttctgcacc acaacgattc aaatgagtcc agtctctaaa caagcctttg tattgcgctc
     1141 tatctgcgat gggcaaggta acagaaccca ccttctccat ccttcgcata acagcgatca
     1201 cacgatcatc tgttccgcga agagcgagag atgcccagac gtctgaaaga ggaatgtaaa
     1261 ccagaaacac ttcaaggccg cgagcgcgaa ggccttgaac gctgcgctcc aatgactcta
     1321 ctgcgcctgg tctatccgca taggcaagaa gcatgggcgg gggtggaggt atatctgcat
     1381 tgcatgtctg gtctgtggac ttccagaccc agaaccctct ttggtcattc agtatctttt
     1441 ctatcttgag gtttgtctct ttccttccag caacaaacga aatcggatct gccgacgcaa
     1501 gagccggaga cattcgatcg cgcaaacgaa gaacaggcaa aaaacgaatc atcctgtaca
     1561 ctgaatcgcc tgtgttcttc ccggacagtg tcatcaaatc aggattcaag tccgcaaaag
     1621 accaatcgta tgtatttcta tagtaactca gaaaggactg cagcacttcg cttttgaaaa
     1681 gaaaaaaagg actaacattg atgatcactc tacggagccc tcgcacctgc cccacgtccc
     1741 gcgccattct ctccactccc tcaggctgct gagaaggg
//
LOCUS       DAORFF010000685         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_57486_length_1775_cov_3.994186, whole genome shotgun sequence.
ACCESSION   DAORFF010000685 DAORFF010000000
VERSION     DAORFF010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57486_length_1775_cov_3.994186"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..576)
                     /locus_tag="PKK76_19125"
     CDS             complement(37..576)
                     /locus_tag="PKK76_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70648.1"
                     /translation="MIRVRKVGFGITLVSLSIFNMNCASFRSDVTLVSPANEYAIDKD
                     FSFSVVGWREGDRTGTFLKEQMGHANDAERAAVRLRASREELDAGSPLRILNAIVTIV
                     SGGIIPFYHPTKTRISYIAFEDGMMRGECSYEIKNNELFSLLMIPFMPFFWPASQQRN
                     MVKETLDKASECFNLKQAH"
     gene            complement(573..1121)
                     /locus_tag="PKK76_19130"
     CDS             complement(573..1121)
                     /locus_tag="PKK76_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70649.1"
                     /translation="MFISQRPAPPSPPEQPVGDVNIEFINWQLPAQVAQAKLIESVFR
                     TAGFFDRVEVQPGGTGWSIEIVNLEYPGEITLGEKFEQRPLQESFGFINRLVAGRTFL
                     LFPIYHPVDRFVRFSVYHRGIHVRDYDYQSRAHIFVGWLTLLATPWSESRELQLEMVT
                     IARTFMAEATRDGLFRREGKSR"
     gene            1287..>1775
                     /gene="fabZ"
                     /locus_tag="PKK76_19135"
     CDS             1287..>1775
                     /gene="fabZ"
                     /locus_tag="PKK76_19135"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNL70650.1"
                     /translation="MAISKTPVKINRIKEILPHRYPFLLVDRVVELTESKIVAFKNLT
                     ANEEFFEGHFPVAPIMPGVLQVEALAQTAGLHYPLAFPEEAEKNKDKIGVFTKVNNCT
                     FEKPVIPGDQLRLEVTITEIEKDKNGDPRRITARGVASVDGKTTCQTDLEFSMIPAKL
                     IGR"
BASE COUNT          513 a          472 c          395 g          395 t
ORIGIN      
        1 gaaacaatgt agcgcaaaga accttcacga gaaacctcaa tgcgcttgct tcaaattaaa
       61 gcattccgac gccttatcca gagtctcttt gaccatgttt ctctgttgcg aggctggcca
      121 gaagaacggc ataaaaggaa tcataagtaa actgaatagt tcattatttt taatttcgta
      181 ggaacactcg cctctcatca ttccgtcctc aaatgcgatg tacgaaatcc tggttttggt
      241 cggatgatag aacggaatga taccgccaga aacaatcgta acgatggcat taagaattct
      301 cagcggactt cccgcatcca actcctccct ggatgcgcgt agacgaacag ccgcacgctc
      361 ggcatcgttc gcatgaccca tctgctcttt caaaaacgtg ccggtacgat ctccttctct
      421 ccacccaacc acagaaaagg aaaaatcctt gtcgatcgca tactcattgg caggcgatac
      481 aagagtaacg tcagagcgaa acgaagcaca attcatgttg aagatagaaa gagagacaag
      541 cgtgatcccg aaaccaacct tgcgtacgcg aatcatctgc tctttccttc gcggcgaaac
      601 aaaccatcgc gcgtggcctc tgccataaac gtgcgcgcaa tcgtaaccat ctccagctgc
      661 aactctctgg attcagacca cggtgtggcg agcaaagtca gccagcctac gaagatatgc
      721 gctctactct ggtaatcgta gtcacgcaca tggatccctc tgtgatagac tgaaaatcgc
      781 acaaagcgat ccaccggatg ataaatggga aaaagaagga atgtgcgacc cgccacgagg
      841 cgatttatga agccgaagct ctcctggagg ggcctttgtt caaatttttc tccaagggtg
      901 atttccccgg gatactccag gtttacaatt tcaatggacc aacctgtgcc cccgggctga
      961 acctccactc tgtcaaaaaa gccagcggtt ctaaatacgc tttcgatcaa cttcgcctgc
     1021 gcgacctgcg caggcaactg ccagttgatg aactcgatat tgacatctcc cactggctgt
     1081 tcgggcggag atggaggcgc cggcctttga gagataaaca cagcacaatc cgaaacgaaa
     1141 gcaaaacatg ccgccgccaa tagtctacag gccgagcatt tcatgcgtca tggtttcccg
     1201 aattgccccc gatagtccag cgattgttcc ggtcgccgaa tccggatgct gattgacaat
     1261 ccaacctacc gtcaccggat cgaaacatgg caatctcaaa aacacccgta aaaataaatc
     1321 gaatcaaaga aattcttcct catcgctatc cgttcctttt agtggatcgt gttgtagaac
     1381 tcaccgaatc taaaattgta gcgttcaaga atctaaccgc caacgaagag ttttttgaag
     1441 gtcacttccc ggtggcaccc atcatgccag gagttctcca ggtagaagcg ctcgcacaaa
     1501 ctgccggatt gcactaccct ctcgcttttc ccgaagaagc agaaaagaat aaagataaga
     1561 ttggggtttt cacaaaagtg aacaactgca cgtttgaaaa gccggtgatc cctggtgatc
     1621 aactccgcct ggaagtaacc attacggaaa ttgaaaaaga caagaacggc gatccaagac
     1681 gaatcactgc acgtggtgtt gcaagtgtgg acggcaaaac aacatgccag actgacctcg
     1741 agttttcgat gattccagca aagctaatcg gacgt
//
LOCUS       DAORFF010000686         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_57621_length_1773_cov_3.823632, whole genome shotgun sequence.
ACCESSION   DAORFF010000686 DAORFF010000000
VERSION     DAORFF010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57621_length_1773_cov_3.823632"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKK76_19140"
     CDS             complement(<1..540)
                     /locus_tag="PKK76_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70651.1"
                     /translation="MRISKIWSRLSQVGVSFEPDPEERKRIILVNKTSITVFLVSIVI
                     PVVPIQFNLDRVALAQFTASFAYLLVPVLNSFHRFFQAKLALMIFGVLHIGVFAHVLG
                     EVSATHFFIFAPALGAYVAFSPRDWRIRTFCTLFSLAVLILLLCDFAGKPLEFKDFWA
                     RIYYYFSLVLALVLCLFLMH"
     gene            complement(564..>1773)
                     /locus_tag="PKK76_19145"
     CDS             complement(564..>1773)
                     /locus_tag="PKK76_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016748095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNL70652.1"
                     /translation="CAAYVSVFGKTADRREEFVFFAVESNHPKYECTQNVVRSQKYVA
                     EFILHDYPITDKEILSRGRDAWDASLATVAFAKFNLGLAAIGICTHAYYEAINHAAAR
                     KLYGTFVTDFPHIRQLFTEAYARLEAMRFFAYRAKDYMKNSSASDRRYLLFNPMVKMK
                     VTMQGEEVINLLWDVIAARGFEKDMYFESAARDIRMLPKLEGTAHVNMVLVIKFMMNY
                     MFETQEYAAVPENNGPSEDAFLFAQGSTTKGLEKVIPGDYSKVYGRFSSPNLNLFKSQ
                     MEDLKAFLSKCASDKEQSKDLDFMLTLGEIFTLVAYGQLVLEQCEIEIAKHSEKKAEL
                     EDLIDLIFDFMVRDFSKHAVTLYGKPSATAAQVQQAIKMIRRPLTETSVFDRVCSRAT
                     GLKEAYRMKE"
BASE COUNT          502 a          428 c          404 g          439 t
ORIGIN      
        1 atgcatcaaa aaaagacaaa gaaccagggc aagcaccagg ctgaaatagt aatagatcct
       61 ggcccagaaa tccttaaact caagcggctt gcctgcaaaa tcgcaaagca gcaaaattaa
      121 aaccgcaaga gaaaacaaag tgcagaacgt gcgaatgcgc caatcgcgcg gagaaaaggc
      181 aacgtacgct cccagagcgg gcgcgaagat aaagaaatgg gtggcggata cttcccccag
      241 cacgtgcgcg aagacaccaa tgtgcagcac tccaaagatc atcaaagcaa gctttgcctg
      301 gaagaatctg tgaaaggaat tcagaactgg aaccagcaag taagcgaaag aagcagtaaa
      361 ttgtgccagc gcaacgcgat ccaggttgaa ctgaatggga acaactggaa tcacgataga
      421 caccagaaat actgtgatac tggtcttatt tacaagaatg attctctttc tttcttcggg
      481 gtctggctca aacgagactc cgacctgaga cagtctgctc cagattttgc tgatcctcat
      541 gagagtttct ttgaaagtaa aactcactct ttcatgcgat aggcttcttt caaacctgtg
      601 gctcgactgc aaacgcgatc gaagacagaa gtctcagtca gaggcctgcg aatcattttg
      661 atggcctgct gtacctgtgc tgcagttgca gacggctttc catagagagt gactgcatgt
      721 ttcgaaaagt ctctcaccat gaaatcaaaa atcaaatcga tgagatcttc caattctgct
      781 ttcttctccg aatgcttcgc aatttcaatt tcgcattgct cgagcaccag ctgaccgtac
      841 gcaaccagag taaagatttc tccgagagtc agcatgaaat ccagatcctt tgactgttct
      901 ttgtccgatg cgcactttga aagaaacgct ttcaaatctt ccatctgtga tttgaaaaga
      961 ttcaaattcg ggctggaaaa tcgaccgtac acctttgaat agtctccggg gatcactttc
     1021 tcaagaccct tggtggtaga accctgcgca aataaaaatg catcttcgga gggcccattg
     1081 ttttctggaa cagcagcgta ctcctgcgtt tcaaacatat agttcatcat gaatttgatc
     1141 acaagaacca tgttcacgtg ggctgttcct tccagcttgg gaagcattcg aatgtcgcgc
     1201 gccgcagact cgaagtacat atctttttcg aaaccgcgcg cagcaatcac gtcccagaga
     1261 agattgatca cttcctctcc ctgcatggtt actttcattt taaccatggg attaaagagc
     1321 agataacgtc tgtcagaagc agaggaattc ttcatataat ccttggcgcg atatgcgaag
     1381 aacctcattg cctccaaccg ggcgtaggct tctgtaaaca actggcgaat gtgtgggaaa
     1441 tcggttacga atgttccgta cagtttgcgc gccgctgcgt ggttgatggc ttcgtaatat
     1501 gcgtgagtgc atattccaat ggccgccaga cccagattga atttcgcaaa agcaactgta
     1561 gccagggatg catcccaggc atcgcgaccc ctggatagaa tttctttgtc tgtgatcggg
     1621 tagtcgtgca ggatgaattc cgccacgtat ttttgtgatc ttactacatt ctgagtgcac
     1681 tcgtatttcg gatggttgct ttctactgca aagaagacga attcttcacg cctgtccgcc
     1741 gttttcccga acacagatac atacgcggca caa
//
LOCUS       DAORFF010000687         1760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_58474_length_1760_cov_2.686804, whole genome shotgun sequence.
ACCESSION   DAORFF010000687 DAORFF010000000
VERSION     DAORFF010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58474_length_1760_cov_2.686804"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKK76_19150"
     CDS             complement(<1..301)
                     /locus_tag="PKK76_19150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70653.1"
                     /translation="MKRVIVIIALFSVPVWAGRKEGVQQEYRAVEEAIRNKQTGPEAR
                     LKTLETNLVNAVRMAMTRHYYEEKETIIKDLSIQTIEYENPTSPLVYFVKYKNSFF"
     gene            complement(364..>1264)
                     /locus_tag="PKK76_19155"
     CDS             complement(364..>1264)
                     /locus_tag="PKK76_19155"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNL70654.1"
                     /translation="LARLEALSVTEGITLYDAMARAGELQGVRGSRAIMDLHSWFENW
                     RGMNPARPSLVLESIIEKTGYLEFLAKDENPGESRIENVEEFASSIQEFEESESEPNL
                     SAYLQQISLYSEQGDAEGQDAVNLITLHNAKGLEYRAVFFTGLEEGTIPHTFSLEDNI
                     EEERRLAYVGITRAREFLYLSSSRFRRIQGSYQPRLPSRFISEIDVSVFEEGIATRAQ
                     NTPRARDSMGSFAGVGRRPPPPSVQVVPVQIGDRIRHKSYGPGTVVEQEDTVAGAKIG
                     IQFDGDEKVRYFLARFAPIEKIE"
     assembly_gap    1265..1274
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1275..1714)
                     /locus_tag="PKK76_19160"
     CDS             complement(<1275..1714)
                     /locus_tag="PKK76_19160"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNL70655.1"
                     /translation="MLRLEENYRSTAPILKAASSLIAHNSERREKTLFTRQPGGEKIH
                     YTVYESDTEEAAGAISRIKSLKQQGIPYAESAVFYRTNAQSRHFEELLRRDGIPYLLV
                     GGFPFYDRKEIKDLIAYLSVVVNPEDSISLMRIINTPARGFGEAG"
BASE COUNT          403 a          452 c          421 g          474 t
ORIGIN      
        1 gaaagaatga gttcttgtat ttcacgaagt agaccagagg agaagtaggg ttttcgtatt
       61 cgatggtctg gatggacagg tctttgatga tcgtctcctt ttcttcgtag tagtgacgag
      121 tcatggccat gcgaaccgcg ttcaccaggt tcgtttccag agtcttaagt ctggcttcag
      181 ggccggtctg cttgttgcga atggcttctt ctactgcccg gtattcttgc tggacgcctt
      241 cttttcttcc ggcccagacc ggaacactga acaatgcaat gattacaatt acccgcttca
      301 tctttacctc gatcggcaac aggatggcgc gaattgagcc ggaagcaacg ataatttccg
      361 ggtttattct atcttctcga tgggagcaaa cctggccagg aagtacctga ccttctcatc
      421 tccatcaaac tgtatgccga tctttgcacc cgctactgta tcttcctgct ccaccacggt
      481 gcccgggccg taggatttgt gtcgaattct atccccgatt tgtacgggca caacctgcac
      541 tgacggaggc ggcggccttc ttcccactcc agcaaaactg cccatagaat ctcgcgcacg
      601 cggagtgttc tgagctctcg tggcgatccc ttcttcgaaa acggatacgt ctatttctga
      661 aatgaaacga gacggaagcc tgggttgata gctgccctga atgcggcgaa atcgactgct
      721 ggacaaatac agaaattcac gcgctcgagt gattccaaca tacgcaagcc tgcgctcttc
      781 ttctatgttg tcctcaagcg aaaaagtatg cggaatggtt ccctcttcca gacccgtaaa
      841 aaacacagcg cgatattcca gacctttcgc gttgtgaagg gtgatcaggt tgactgcgtc
      901 ttgaccctca gcatctccct gctctgaata gagagagatt tgctggagat acgcactgag
      961 atttggttca gattcgcttt cctcgaattc ctggatggag gaagcgaatt cttctacgtt
     1021 ttcgatcctg gattcaccgg ggttctcatc tttcgcaagg aattccagat atcctgtctt
     1081 ttctatgatg gattcaagca ccagagaagg tcttgctgga ttcatcccgc gccagttttc
     1141 aaaccaggaa tgcaggtcca tgatcgcgcg cgaaccacgc acaccctgga gttcccccgc
     1201 tctcgccata gcatcgtaga gagtgattcc ttctgtcaca gaaagcgctt ctagacgagc
     1261 caggnnnnnn nnnncccgcc tctccaaaac cacgggccgg agtattgatg atgcgcatca
     1321 agctgatgct gtcttccgga ttcacaacca cggacagata ggcgattaaa tctttgattt
     1381 cctttctgtc gtagaaggga aagcctccca cgagcaaata cggaatgcca tctcgtctta
     1441 gcaattcttc aaagtgtctg gattgcgcgt tggtgcgata aaacaccgcg gactccgcat
     1501 atggaattcc ctgctgcttg agactcttga tacgcgaaat ggctcccgcc gcttcttctg
     1561 tgtcagactc gtaaactgtg taatgaattt tctctccacc tggctggcgg gtaaagagtg
     1621 tcttttcgcg acgctcactg ttgtgcgcga tgagagaaga agctgctttg agtatgggcg
     1681 cagtgcttct ataattttct tcgagacgaa gcactgtgca atttggataa tctttttcaa
     1741 aagaaagaat attctcaata
//
LOCUS       DAORFF010000688         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_58890_length_1753_cov_3.841578, whole genome shotgun sequence.
ACCESSION   DAORFF010000688 DAORFF010000000
VERSION     DAORFF010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58890_length_1753_cov_3.841578"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PKK76_19165"
     CDS             <1..888
                     /locus_tag="PKK76_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70656.1"
                     /translation="SVWSLPIVMWRRDDYLFIFPYYHSEGTERSNHLLPLIYTWTTDT
                     DFTGIFAGLYVRSYENYNRQNFLFLLDHTHREDYNEFEALLGAVHIANSRNQFDMKLL
                     YGTLFSLRSSRDPSEFDLDFALYLGGLQFKPESTHSRLLPVYWYDHDRTSTFFASPVL
                     TYDSSQTSRTVWSVPLLFYYNKDETSRTIWSIPWIELESDSGARLFQLGLLGSVWYRN
                     YDRYERTDRKLALLGIAYQDVERPERGYRSRGSLWGLLWDYETEEETGFNKVSVLKVL
                     YSRTENRGDVKHKVFGITL"
     gene            885..>1753
                     /locus_tag="PKK76_19170"
     CDS             885..>1753
                     /locus_tag="PKK76_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70657.1"
                     /translation="MNARLSTTTARELFSMQMHARLLPGIASYLTIVLTFVVLLPLEA
                     LRLFHTGFYAGDLLFAVEINLVAAAVIAALAQVLFFSMRILAALLMRFEWIAVSLLPG
                     VGVFLFFQAYVDSLATQHLPAVHPLIVWAATLTVGLLLSLPLLAVFPEIRGENSPLFL
                     FSVLLSRGFMLFSDVSGFLSTPVEVFLQFLVFCFLYIWLQLRRRIHRSPHYSPFVVPA
                     LQELGVFVVWLILYAASGVFPFVLPSFDTTVLTMATGIGISWSLGARFLGSQLQGAGG
                     GNAPWLAALSFILA"
BASE COUNT          346 a          439 c          422 g          546 t
ORIGIN      
        1 agtgtgtgga gtcttcccat tgtgatgtgg agaagagacg attatttgtt catatttcca
       61 tactatcaca gcgaaggaac agaacgatcc aatcacctgc ttcctctcat ttatacctgg
      121 accacggaca cggactttac tggaatcttt gcgggactgt acgtgcgatc ttatgaaaac
      181 tacaacaggc agaattttct gttcttactc gatcacacac atcgtgaaga ctacaatgaa
      241 tttgaagcgc ttctcggtgc ggttcatata gccaacagca gaaatcagtt tgatatgaaa
      301 ctgctgtacg gaacgctatt ttctcttcgc tcatccagag atccgtctga gtttgatctg
      361 gattttgctc tctatcttgg tggtcttcaa ttcaaacccg agtcgactca cagtcgtcta
      421 ttgccggtct attggtatga tcacgaccga acctctactt tcttcgcaag tcctgttttg
      481 acgtacgata gttcgcagac ttcgcgtaca gtgtggagcg ttcctttgct tttctactat
      541 aacaaggatg aaacttcgcg tacgatctgg agcattccgt ggattgaact cgaatccgat
      601 tcaggcgcga gactttttca gctcggcttg cttggatctg tctggtatcg caattacgat
      661 cgatacgaaa gaaccgatcg caagctagct cttctgggta tcgcctacca ggatgtagaa
      721 cgaccggaac gtgggtatcg ctccagagga agtctctggg gacttctctg ggattacgaa
      781 acggaagaag agacaggatt caataaggtg tccgtgctga aagtactgta cagcagaacg
      841 gaaaatcgcg gagatgtgaa acacaaagtc ttcgggatta ccctttgaat gcccgcctct
      901 ccactactac cgctcgtgaa ttgttctcca tgcagatgca cgcacgtttg cttccaggaa
      961 ttgcgtccta tcttaccatt gttctgacgt ttgtcgttct gttgccgctg gaagcgctcc
     1021 gactctttca taccgggttc tacgctggag acttgctttt cgcagtggag atcaatttag
     1081 tagcggccgc cgtcatcgct gcgctggctc aggtgctttt tttctccatg cgaattctgg
     1141 ctgccctgct gatgcggttt gaatggatcg ccgtgagtct cctccccggt gtcggtgtat
     1201 ttttgttctt tcaggcttat gtagattctc tggcaaccca gcatcttcca gctgttcatc
     1261 ctctgattgt atgggcggcc acattgaccg ttggactttt gctttcgctt ccgcttctgg
     1321 cggtctttcc tgaaatccgt ggggagaaca gtcctttgtt cttattttca gtcttgctca
     1381 gtcgtggatt catgctcttt tcagacgttt ccggtttcct gagcacgcct gtcgaagtgt
     1441 tcctgcaatt tcttgtcttc tgttttctgt atatctggct tcaactcagg cgccggattc
     1501 atagatcccc gcactattcg cctttcgtgg tgcccgcctt acaagagtta ggtgtttttg
     1561 tagtctggtt gattctgtac gcggcctccg gcgtatttcc ttttgttctt ccgtcttttg
     1621 acactaccgt cctcacgatg gccaccggca ttggaatttc ctggtctctg ggcgcccgct
     1681 tcctggggag ccagctgcag ggtgctggag gaggcaatgc accatggttg gctgctttgt
     1741 ctttcattct tgc
//
LOCUS       DAORFF010000689         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_59165_length_1749_cov_2.774498, whole genome shotgun sequence.
ACCESSION   DAORFF010000689 DAORFF010000000
VERSION     DAORFF010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59165_length_1749_cov_2.774498"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="PKK76_19175"
     CDS             <1..1258
                     /locus_tag="PKK76_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HNL70658.1"
                     /translation="FPRETIPVLTLANADVAFHRRGFFLRKDIKRVSDHWTWPDVSMD
                     QDRRIELFTALARSEAADKLFSFCGLSGEQRAIARSLLVRFFCDFFPAQFLEGVGKNH
                     AIADDLLREFQTRALISSGDGSSHSMFVIGAARARGFKIVKFQHGGHYGYYRDFTHML
                     EVEFPDTDIFLSWGWTRLPEHPEFSSLQVLPMPGPWMSERRLYWSKYRIGLGRRFDML
                     WMPQMMKRFPGAPQGASSVRRDVLQSFSAEMIDVARHANERKVRIHCKPYNALTLYLM
                     RETYAQLETVGGEYFDRAEHFDKGLTMETLNHASLVLWDQPGTGFLECLQCGIPTMIL
                     WSRLYCEEEEWCVGDFKALEDAGIVHRTASTLFDEYARFKLDPHAWMKKNSSVVSRFV
                     ARYGHAVNHWSSSWSHLLKQLRRGKL"
     gene            1255..>1749
                     /locus_tag="PKK76_19180"
     CDS             1255..>1749
                     /locus_tag="PKK76_19180"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNL70659.1"
                     /translation="MTDPSFTFEQLAASAGIRTDGKSRPVVCVQGLGFVGAAMSVAVS
                     EAADPQGNPRFNVVGIDLPGPRGERVIRSLNEGKFPAETDDANLVAATSRAYRRGNLV
                     ATADAEAFRLASVVIVDINLDLGPTSADGEPFVDFSGLRAAIRTFATRISPDCLVLVE
                     TTVPP"
BASE COUNT          378 a          436 c          482 g          453 t
ORIGIN      
        1 gtttcctcgc gaaaccatcc ctgtactgac acttgcgaac gcggacgttg catttcatag
       61 gcgcggattc tttcttcgaa aagacatcaa gagagtttct gatcactgga catggccgga
      121 tgtatccatg gatcaggacc gtcgcattga gctcttcacc gcgttggcgc gaagcgaagc
      181 tgcggataaa ttattctctt tctgcggact ttcaggggaa cagcgagcga tagcgcggtc
      241 tcttctggta agattctttt gcgatttttt tcccgcgcag tttttagaag gcgttggtaa
      301 gaatcatgca atcgcggacg acttgctccg ggagttccaa acccgtgcgt tgatttcatc
      361 cggggatgga tcgtcgcatt ctatgttcgt gatcggcgcg gcgcgcgcca ggggtttcaa
      421 gatcgtgaag tttcagcacg gtggacacta cggttattat agagatttta cgcatatgct
      481 ggaagtcgaa ttccccgaca ccgacatctt cttgagctgg ggatggacaa ggcttccaga
      541 gcatccagaa ttctcttcac ttcaagtatt gcccatgcct ggtccctgga tgtcagaacg
      601 tagactctac tggtcgaaat accgcattgg tctgggtcga cgctttgaca tgctctggat
      661 gccgcaaatg atgaaacgtt ttcctggcgc tcctcagggg gcttcttccg tgcgtcgcga
      721 cgtgctgcag tcattctctg cggaaatgat cgatgtggcc cggcacgcca atgagcgaaa
      781 ggttcgcatt cactgcaaac cgtacaacgc tctgacattg tatttgatgc gagagactta
      841 tgcgcaactc gaaacggtgg gcggtgaata ctttgatcgc gcagaacatt ttgacaaggg
      901 tctgaccatg gaaacgctca atcatgcgtc gctggttctc tgggatcagc caggcaccgg
      961 gttcctggaa tgccttcagt gcggaattcc aactatgatt ctatggtcga gactctactg
     1021 cgaagaggaa gaatggtgtg taggagattt caaagcactt gaggacgcgg gcattgtgca
     1081 cagaactgcg tccactttgt ttgacgaata cgcacgcttc aagttagatc ctcacgcctg
     1141 gatgaagaag aattcttctg tggtatctcg ctttgtggct agatatggtc atgctgtgaa
     1201 tcactggtcg agctcctggt cgcatctcct gaaacaactt cgtcgaggga aattatgaca
     1261 gatccttctt tcacctttga gcagttagct gcttctgctg gcattcgcac agatgggaaa
     1321 tccaggcccg tcgtctgtgt ccagggtctg ggcttcgttg gcgcggcgat gtctgtggcg
     1381 gtgtcagagg ccgcagatcc tcagggcaac ccacgcttca atgtcgtagg aatagacctg
     1441 cccggtccac gcggagaaag agtgattcgg tctctcaacg aggggaagtt tccagctgag
     1501 acagacgacg caaaccttgt agcggcaact tccagggcat acagacgcgg caatcttgtt
     1561 gccacggcgg atgcggaagc ctttcgcctc gcgagcgttg tgatcgtgga catcaatctt
     1621 gatcttggcc cgacttctgc agatggagag ccatttgtag atttctccgg attgcgcgcg
     1681 gccattcgta cgtttgcaac tagaatttcg cccgactgtc ttgttctggt agaaacgaca
     1741 gttccaccc
//
LOCUS       DAORFF010000690         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_59460_length_1744_cov_3.437537, whole genome shotgun sequence.
ACCESSION   DAORFF010000690 DAORFF010000000
VERSION     DAORFF010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59460_length_1744_cov_3.437537"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKK76_19185"
     CDS             <1..638
                     /locus_tag="PKK76_19185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70660.1"
                     /translation="QVQVRYVDADARNRLRAVFENLEADLKIDGIGVVLTTAVLELVE
                     NAVKANLKRAFFSKNTYNFEDPTSYTAGIESFVKSYPDIRQGHYDSALKDLELVVSVQ
                     VDYNDHRLLVYVENNAVLVSQEEIRIRKQLAAAMLSDKIAEFYVQYGDETEGSGLGLA
                     MIVVLIRNLGFDAHNFRVFQKDNRTIARLEFPLNADYVPIRERWRREHPAG"
     gene            complement(580..>1744)
                     /locus_tag="PKK76_19190"
     CDS             complement(580..>1744)
                     /locus_tag="PKK76_19190"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL70661.1"
                     /translation="NVMNRREPVILNDASQDETFNMGDLTIRSALCMPLLVKGNVAGV
                     LYLDNDLATGVFTEEKLGFLSVLTSQFAVSLENAALYSGLETRVQERTAELENSLEQV
                     RTLKLAQDLDYYLTALLLQPLSGIRITGEGLAIQQHVLQKKKFKYVRWEEQLGGDICI
                     ARDVRIGGRVCTAIVNADAMGKSMQGAGGALVFGAVFESITASSYEDSGPEQWLRNCY
                     TELNEVFRSFSGYMMISAVISLVDTKARTIFSINADHPLPVLVRDGHASFQPQTVLPR
                     FGMRPTPPEIEINVTQANEGDIFILASDGRDDLIVPGPDGPTLNEDPDLFLHTVEDNA
                     GDAETIFKTLCAGDRLTDDLSIISVTFPAAASHNLSQQDARDASVRESEHSPH"
BASE COUNT          405 a          426 c          456 g          457 t
ORIGIN      
        1 aacaggtgca agttcgatac gtggacgcgg acgcgcgaaa tcgtcttcgg gccgtttttg
       61 aaaacctgga agcagacctg aagattgatg gcattggagt agttctgacc accgctgtgc
      121 tcgagctcgt ggagaatgca gtcaaagcca acttgaagcg cgcgtttttt agcaagaata
      181 cttacaattt tgaagatcca accagttaca ctgctggaat tgagtctttc gtaaaatcgt
      241 atcccgatat tagacagggt cactatgatt ctgccctcaa ggatctggaa ctggtggttt
      301 ccgttcaggt agactacaat gatcacaggt tgctggtgta cgtggagaac aatgcagttc
      361 ttgtttcgca ggaagaaatc aggattcgca agcaactcgc agctgccatg ctttcagaca
      421 agatcgcgga attctacgtt cagtacggcg acgaaacgga gggaagtggt cttggcctgg
      481 ccatgattgt ggttttgatc cgcaatcttg gttttgatgc tcacaacttt cgtgtattcc
      541 agaaggacaa cagaaccatc gcacgcctcg aatttcctct caatgcggac tatgttccga
      601 ttcgcgaacg ctggcgtcgc gagcatcctg ctggctaaga ttgtgtgacg ccgcggctgg
      661 aaaagtcacg gagatgatag acagatcatc tgtgaggcga tcccctgcgc agagagtttt
      721 gaaaattgtt tctgcatctc cggcgttgtc ttctacggta tgtagaaaaa gatccggatc
      781 ttcattgaga gtgggcccgt ctggtcccgg cacgatcaga tcgtcgcggc cgtccgatgc
      841 aagaatgaat atgtcccctt cgttcgcttg cgttacgttg atttcaattt ctggcggagt
      901 gggtctcatt ccaaatcgag gcagtacggt ttgaggctga aaagatgcgt gtccgtctcg
      961 aaccagtacg ggcaatggat ggtctgcgtt gatactgaaa atggttcgtg cctttgtgtc
     1021 cacgagtgaa ataaccgcgg aaatcatcat atagccgctg aagctgcgaa acacttcgtt
     1081 cagttctgta taacagtttc gcaaccattg ttcgggtccc gaatcttcgt aggaggatgc
     1141 tgtgatcgat tcaaacactg cgccaaatac gagcgcgccc cccgcaccct gcatggattt
     1201 tcccattgca tctgcgttta caattgcggt gcaaactctg ccaccgattc gcacatcccg
     1261 tgcgatacag atgtctcccc ccagttgttc ttcccagcgc acgtatttga atttcttctt
     1321 ttgcaaaacg tgttgctgga ttgccaggcc ttcgccagtg attcgaatgc cgcttaacgg
     1381 ttgcaagagg agtgcggtca agtagtagtc caggtcctgg gcaagcttca gtgttcgaac
     1441 ttgttccaga gagttttcca gttccgccgt gcgctcctgg actctggttt cgaggcctga
     1501 gtagagcgcc gcattttcga gagagactgc gaattgtgag gtaagcacag agagaaatcc
     1561 aagcttttct tccgtgaaaa cacccgtggc aaggtcgttg tccagataca acacccccgc
     1621 aacattgcct ttcaccagca gtggcataca cagtgccgat cgaatggtca gatcgcccat
     1681 attgaatgtt tcgtcctggg aggcatcatt tagaatcacg ggttcgcgcc tgttcatcac
     1741 gttg
//
LOCUS       DAORFF010000691         1734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_60109_length_1734_cov_3.398451, whole genome shotgun sequence.
ACCESSION   DAORFF010000691 DAORFF010000000
VERSION     DAORFF010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60109_length_1734_cov_3.398451"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PKK76_19195"
     CDS             complement(<1..321)
                     /locus_tag="PKK76_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNL70662.1"
                     /translation="MRPLSDSEQKRTREILERISAIPDSEWQHAKEMLITNDYPAGSF
                     LLRAGENPMQSFVLLEGAVRVYYVSRGGREYNRAFLAEGGIAASMRSVIAAEPSHLFI
                     QAMVP"
     gene            complement(576..1145)
                     /locus_tag="PKK76_19200"
     CDS             complement(576..1145)
                     /locus_tag="PKK76_19200"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNL70663.1"
                     /translation="MKHTYAIMRHGQSQANVEGIIVSDPAIGVARYGLTEAGKKQALE
                     SARSSDFKARAIYSSDFRRARETAEIVAALQKAPVTETELLRERFFGDYNGTPDTQYQ
                     RVWADDSASIEHGRGVESPFSVLGRVLSLVETIESSILAPTPVLLVAHGDVCQILLAH
                     CAGLSPFLHRSIPHMQTAEIRRIRFPENP"
     gene            1233..>1734
                     /locus_tag="PKK76_19205"
     CDS             1233..>1734
                     /locus_tag="PKK76_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002108027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNL70664.1"
                     /translation="MIGPRRIVCLTEETTELLYLLGEEERIVGISAYTVRPPQARNEK
                     PIVSAFLNGSIPKIKALEPDLIIGFSDIQADLAKSLIAEGLNVLVTNQRSIEEIFETM
                     QMVGAIVGKGEDTARWIGSFRAKLDSVQVEGERRFRVRSAKPRVFFQEWDEPLITGIR
                     WVTELVE"
BASE COUNT          410 a          415 c          483 g          426 t
ORIGIN      
        1 cgggaccatt gcctggataa agagatgaga aggctcagcc gcgatgacac tgcgcattga
       61 ggctgcaatt cctccttccg cgaggaacgc acgattgtat tcacgtccac cgcggcttac
      121 gtagtacact cgcactgccc cttccaggag cacaaatgat tgcatcggat tctcacccgc
      181 gcgcagcaag aagctgccag ccgggtaatc gtttgttatg agcatttctt tcgcgtgttg
      241 ccattcagaa tctggaatcg ccgagatgcg ttccagaatt tctcgagttc gtttttgttc
      301 tgaatcgctg agaggtcgca cggatcctga tcgagtcccg ttggccaaag acaagtagaa
      361 agcgagcccg attgggatcg cgccgctcgc ctggaagcgt ggtaagtccg gcaaaacggc
      421 caggctaatg cgacattcgc cggattatgt cacattacgg gccgcaaaaa tggtttgaca
      481 cggaggaaaa agccgccctc cgaagagaca taatccgggc gaattatgtc tcataaatta
      541 tgctgaatat tgaatttact taaagttaat gaaatttatg gattttccgg gaatcggatt
      601 cgtctgattt ctgcggtttg catgtgcgga atgctgcgat gcaagaaagg agaaagtccg
      661 gcgcaatgtg ccagaagaat ctggcaaacg tctccatgtg ccacgagaag taccggtgtg
      721 ggtgcaagga tagacgactc tatggtctca accagagata gaactcttcc cagcacggag
      781 aaaggagatt ccactccgcg gccgtgttcg atggaagcgc tatcatccgc ccagactcgc
      841 tggtactgtg tgtctggtgt tccattgtaa tcgccaaaga aacgctcgcg aagcaattct
      901 gtttcagtca ctggcgcctt ctgcaaagcc gcgacgatct ctgcggtctc cctggctctt
      961 ctgaagtcgg aagaatagat tgcccgggct ttgaaatcag aagagcgagc gctttcgaga
     1021 gcttgcttct ttccggcttc tgtgaggccg tagcgtgcaa ctccaatcgc aggatcggac
     1081 acgatgattc cttctacgtt agcctggctc tggccgtgtc gcatgattgc ataggtgtgt
     1141 ttcatcttgc ccttccaact ttatgcgacc aggccaaatc ggcacatcct aaaatggatt
     1201 gcacggagtg gatctgtgtt cgatgttacg gcgtgattgg cccgcgtagg atcgtttgtc
     1261 taacagaaga aacaaccgag cttctgtatt tgctggggga agaggagcgc atcgttggaa
     1321 tttccgccta cacggtgcgg ccgcctcaag cacgaaatga aaagcccatt gttagcgcgt
     1381 ttttgaatgg cagcattcct aagatcaagg cacttgagcc tgatttgatc atcggtttct
     1441 ctgatattca ggcggatctc gctaaaagtc tcatcgcaga aggattgaat gtgctcgtga
     1501 ccaatcagag atccattgaa gagatctttg agaccatgca gatggtgggt gcgatcgttg
     1561 ggaaaggaga agataccgct cgttggattg gctcgtttcg tgcgaaactg gattcagtgc
     1621 aggttgaggg cgagaggaga tttcgtgtgc gatcggcaaa gccccgtgta ttttttcaag
     1681 aatgggacga gcccctcatc acagggattc gctgggtgac cgagctcgtg gagg
//
LOCUS       DAORFF010000692         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_60228_length_1732_cov_3.463924, whole genome shotgun sequence.
ACCESSION   DAORFF010000692 DAORFF010000000
VERSION     DAORFF010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60228_length_1732_cov_3.463924"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="PKK76_19210"
     CDS             complement(<1..1017)
                     /locus_tag="PKK76_19210"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL70665.1"
                     /translation="MARSLQFIGGTGTVTGSKYLITTKNNRILVDCGLFQGLKVLRER
                     NRKPLPLSPRSISHVVLTHAHLDHSGYVPLLVKKGYSGEILSTRATKALCGILLPDSG
                     RLQEEDAHYANKHQFTKHEPALPLYTEKDARHCLKYFRTVEYHQNIKLSKNISFEYFR
                     AGHIPGSCIVKFNLGGETVVFSGDLGRPINNLLPQPDRVTDADVLVLESTYGDRVHDR
                     EDPEVLLERLINKTVQRKGRILIPAFAVGRTQEILHFVIRLKKQNRIPQIPIYVDSPM
                     ATEATRVFLSFSEENRLTAEEAADMRAHARFVDTTEESRALNDSKESAIIISASGMAT
                     GGRVL"
     gene            complement(1017..>1732)
                     /locus_tag="PKK76_19215"
     CDS             complement(1017..>1732)
                     /locus_tag="PKK76_19215"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNL70666.1"
                     /translation="VILEKTIGAVHVRAGGSIFLSEGVIGRGAASLQAGGQILSRFVQ
                     SAALHAGGSIVIEDAVLHSRLVSGKDISVEQGKGEILGGEAICSGIFRANRIGSRLET
                     PTRIVLGLDPDTLEKLRALDRDIYEKKRILDRVESHLKQLEEARQRRKEVDLDTESKL
                     LELREKQSSIVNALEKQRRKLQDRIAPNPKSALHTLEAHPGVEVYFGVGIAPYRIFGR
                     PVRNLKLTLADGKIHAGKI"
BASE COUNT          403 a          435 c          425 g          469 t
ORIGIN      
        1 cagtacgcga ccacccgtag ccattccgct tgcagaaatg atgatagcag actctttgct
       61 gtcgttgagg gctcttgatt cttctgttgt atccacaaac cgtgcatgcg cgcgcatatc
      121 agcggcttct tcagccgtca ggcgattttc ctcggaaaaa gacaaaaaca cccgggttgc
      181 ttctgttgcc atggggctat ccacgtaaat cgggatctgg ggaattcgat tttgtttttt
      241 gaggcgaatg acaaagtgaa gaatttcctg agtgcgtccc actgcgaaag ccgggattaa
      301 gattctacct ttacgctgta cggtcttatt gatcaagcgc tcgagcaata cttcggggtc
      361 ttctctgtca tgaacgcgat ccccgtaggt ggattcgaga accagcacgt ccgcatctgt
      421 tacacgatct ggctgtggca gaagattgtt gatggggcga ccaagatctc ccgaaaatac
      481 cacagtttct ccgcccagat tgaattttac aatgcatgag ccggggatgt gtcccgcacg
      541 aaagtattca aatgatatgt ttttacttag ttttatattc tgatgatact ctacagttcg
      601 aaaatacttc aagcaatgac gagcatcttt ctccgtatac aatggaagag caggctcatg
      661 ttttgtgaac tgatgcttgt ttgcgtagtg agcatcctct tcctgaagac gaccagaatc
      721 tggaaggaga atgccgcata acgctttggt tgcacgcgta gagagaatct ccccgctgta
      781 gcccttctta acaagaagag gtacataccc gctatgatcg agatgcgcgt gggtgaggac
      841 aacatggctg atgctgcgcg gagatagcgg aagaggctta cggtttcttt cgcgaagtac
      901 cttgaggccc tgaaacaggc cacaatccac gagtatcctg ttgttcttcg tagtaatcaa
      961 atacttggat ccagtgactg ttcctgtgcc accaatgaat tgaagagaac gagccattat
     1021 atttttcctg cgtggatttt tccatccgcc agagtgagtt tcaaattgcg aacgggcctt
     1081 ccaaaaatgc gatagggtgc gattcccact ccaaagtata cttccacgcc cggatgagct
     1141 tccagggtat gcagcgcgga ttttggattg ggagcgattc gatcctggag cttgcgtctc
     1201 tgtttttcga gcgcatttac aatgctcgat tgtttttcac ggagttcgag gagtttggat
     1261 tctgtgtcca gatccacttc ctttcttctc tggcgagctt cttccagttg cttgagatgg
     1321 ctttccaccc ggtcgaggat tcttttcttt tcgtagatat ctcgatccag ggcccgcaat
     1381 ttctcgagcg tatccggatc cagcccaaga acgattctgg tgggtgtttc cagtctggac
     1441 ccgattctat ttgcgcgaaa gattccagaa cagattgctt ctccacccag gatttcgcct
     1501 ttgccctgtt ccacggaaat gtctttaccc gatacaagcc tcgaatgcaa gacagcatcc
     1561 tcaatcacaa tggacccgcc tgcatgaagg gcagcagact gaacaaaacg cgaaaggatc
     1621 tgtccgcccg cctgaaggct ggcggctccc ctgccaatga ctccttcaga taagaagatg
     1681 gacccgccgg ctcgcacgtg cacggctcca atcgtttttt ccaggatcac gt
//
LOCUS       DAORFF010000693         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_60610_length_1726_cov_3.751047, whole genome shotgun sequence.
ACCESSION   DAORFF010000693 DAORFF010000000
VERSION     DAORFF010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60610_length_1726_cov_3.751047"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PKK76_19220"
     CDS             <1..193
                     /locus_tag="PKK76_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70667.1"
                     /translation="RLFRHRTVFVIAHRLSTIEKADLIVVLSGGQIVDMGKHSDLMQK
                     GGLYARLQEISRQAVPGLA"
     gene            216..635
                     /locus_tag="PKK76_19225"
     CDS             216..635
                     /locus_tag="PKK76_19225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNL70668.1"
                     /translation="MKPVLLVDDNDRYAEMLDKYFRDRGYTPERAFNGKDALGMFKGK
                     PADYYRAIVTDITMESQLAGLSFLWEIKKLGFHGTIVVASTGFDFPLAMPLSRMVLTG
                     WGVHYLVPKAPMKRAGIIEFYPVSLFSSTLKEFQEVQ"
     gene            674..>1726
                     /locus_tag="PKK76_19230"
     CDS             674..>1726
                     /locus_tag="PKK76_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70669.1"
                     /translation="MTNFRRVLIISSLASLLTCVPGIPVKIPRYPEGVMGDQLKSVMG
                     AKRKVGVVAASPNPNLLQQIGYDQDWSATVEGAVTTELTQRGFYTMIDTSSRKQRLRE
                     LAYTQTGLTGEQRNIGQELAAEGLLFIRMTAPPLKECKTEYVTDFAATALKLGMKMAM
                     KDSNGAGDAQEKKPTGVLFLTVFVQGTLTNLETGQSVTYAHSKPYKLQNEAGNQECPS
                     TLDAFGKALQDASAAIADNLSPSIVTVKIPLASDADDLDGDQKAKVTKYLTEGIKWAE
                     ADDFQMAAQNWEKALDASGGKSASALWNIAAYKWYSGDMDGAEKYFKRSFSNGGSDWA
                     DAAKRSLWATFRKEKDR"
BASE COUNT          440 a          436 c          459 g          391 t
ORIGIN      
        1 ccggcttttc cgccatcgca cagtttttgt gattgcacac cggctttcta ccattgaaaa
       61 agccgatttg attgtcgtct tgtccggcgg ccagattgtg gacatgggca agcattcaga
      121 cctgatgcag aagggtggac tgtacgcacg gctgcaggag atttccaggc aggctgttcc
      181 aggtttggcc tgactttatt ttcgaggatt gtagattgaa accagtttta ctcgtggatg
      241 acaatgatcg ctacgcagag atgctagata agtattttag agacagaggc tacactccgg
      301 agcgcgcttt caacggaaag gacgcacttg gaatgttcaa agggaaacct gcggactact
      361 atcgcgcgat tgttacggat atcaccatgg aaagccagct tgccgggctg tcttttctct
      421 gggagatcaa gaagcttggc tttcacggca caatcgttgt ggccagcaca ggattcgatt
      481 tccctctcgc catgcctctt tcgcgcatgg ttttgactgg ctggggagtg cactatcttg
      541 tgccaaaggc tcccatgaag cgggccggaa tcattgaatt ttatccagtt tccctgtttt
      601 cttcgaccct gaaggagttt caggaagtcc aataattgat ttacaaccaa cttcaaaaag
      661 gctcaaaaga ctcatgacga atttccgccg cgtactcatc atttccagcc tggcttcttt
      721 gctcacttgc gtgccgggga ttccagtgaa gattcctcgc tatccagaag gcgtcatggg
      781 cgatcagctc aagagcgtca tgggcgcgaa gcgcaaggtc ggcgtggttg ccgccagccc
      841 caacccgaat ctactgcagc aaatcggata tgaccaggac tggtctgcca ctgtggaagg
      901 tgccgtcaca acggaactga cccagcgcgg tttctacacc atgattgata catcgagtcg
      961 caagcaaaga ctgcgcgagc tcgcttacac tcaaactggt ttgaccggcg aacaaagaaa
     1021 catcggccag gagctcgctg cagaaggact gttgttcatt cgcatgactg ctcctcctct
     1081 caaagaatgc aaaactgaat acgtaaccga tttcgcggcc acggctctga agcttggaat
     1141 gaagatggcc atgaaagatt ccaatggtgc gggagacgcg caggagaaaa agccaactgg
     1201 cgttttgttc ctcacagttt tcgtgcaggg cactcttacg aatctagaaa caggccagtc
     1261 ggtaacatac gcgcacagca aaccatacaa actccagaat gaggccggga atcaggaatg
     1321 cccatccact ctcgatgcgt ttggaaaagc tctgcaggat gccagcgcgg ccatcgcaga
     1381 caatctttcg ccttctatcg taactgtgaa gatccctctt gcatcggatg cagatgacct
     1441 ggacggagac cagaaagcaa aggtgaccaa atatctgacg gaaggaatca agtgggcaga
     1501 agcagacgat ttccagatgg cagcgcaaaa ctgggaaaag gcgctcgatg cgtccggcgg
     1561 caaatcggct tccgctctct ggaacatagc agcttataag tggtattctg gagatatgga
     1621 cggagcggag aaatacttca aacgctcctt ctccaacggt ggatcagact gggcggacgc
     1681 ggccaagaga agtctctggg ctacgttccg caaagagaaa gaccgt
//
LOCUS       DAORFF010000694         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_61642_length_1711_cov_3.859300, whole genome shotgun sequence.
ACCESSION   DAORFF010000694 DAORFF010000000
VERSION     DAORFF010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61642_length_1711_cov_3.859300"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PKK76_19235"
     CDS             <1..755
                     /locus_tag="PKK76_19235"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNL70670.1"
                     /translation="AYCPHLGADLSDGCITQGKIRCAFHHWTFRVDGKCETGERNATS
                     YPVMEKHGAIWLFNGPSPLFGIPEMDQMHATILKPRVIRAHPHLLTPNGLDIEHFSSV
                     HGIHFSKQPVFKTEPFGIAVDMEIDLNASKSFRMARLFAGNQIRAKFYTLGGHISVIR
                     ANAGAVPLHVMFTHRPQPDGTSISRTLLFFPEKMKAPSTLYRFLSGLFLYFIVRDDVR
                     LLEKVQFRSENLKTGEGLALFRDQINQMETMA"
     gene            752..>1711
                     /locus_tag="PKK76_19240"
     CDS             752..>1711
                     /locus_tag="PKK76_19240"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNL70671.1"
                     /translation="MNLYLNPATWEKPAWPGLRDPRWAVFCLLSAYLTLGITVLGFTR
                     TPLQIIVALSAGALLDVIFCGLLKGKWIFPLSAVITTLSLTIILSFSSGYHFIWLPVL
                     IAIASKYIFTLNGRHIYNPSLFAIVFSLIFADGWISIAPAYQWTGSAETAWLMSITIL
                     TAGVTIFGYRGSRKWLALSFLTFFAIQLLIRSYIIRYHLPASALFIGTITSPSFYLFT
                     FFMITDPATSPPDKRTQILVGFFLAIFDLAYHLKFSLFTFFYAAFTLATIRYVVAAWK
                     TRAWSGALRSHIPAAILTFFFAIPAFAMYKSGQSNVRGSAPPGL"
BASE COUNT          397 a          454 c          364 g          496 t
ORIGIN      
        1 atgcatattg tcctcatctc ggagcggatc tatctgacgg ttgcatcacg caaggaaaga
       61 tacgctgcgc ctttcatcat tggacatttc gagtggatgg aaaatgcgaa acaggagaac
      121 gaaacgcaac ttcttatccg gtgatggaaa agcacggagc gatctggttg ttcaatggac
      181 cttcaccgtt attcggcatt ccagaaatgg atcagatgca tgctacaatt ttgaagccgc
      241 gcgtcatacg cgctcaccct cacctgctta cgccgaatgg actcgacatt gaacatttct
      301 cttcagttca cggcattcat ttttccaaac aaccggtttt caagacagaa ccatttggaa
      361 ttgcggtgga tatggaaatt gatctgaacg cttcaaaatc ctttcgcatg gccaggctct
      421 tcgcaggcaa tcaaatcaga gcgaaattct acactctggg cggtcatatc tctgtgattc
      481 gcgccaatgc gggagccgta cctctccatg tgatgtttac tcacagacca cagccggacg
      541 gcacaagcat cagcagaacg ctgctcttct ttcccgaaaa aatgaaagca ccttctactc
      601 tctatcgttt cttgtcgggg cttttcttgt atttcattgt gcgcgacgat gtgcgccttc
      661 tagaaaaggt tcaattcaga tcagaaaacc tgaaaacagg cgaaggcctc gctctgttcc
      721 gcgatcaaat caatcagatg gaaacgatgg catgaatctt tacttgaatc ccgccacctg
      781 ggaaaaacca gcgtggcccg gactgcgcga tcctcgctgg gccgtattct gtctgctctc
      841 agcctacctg actcttggaa tcactgtgct tggattcact cgcactcctt tgcaaatcat
      901 agtcgccctg agcgcagggg cacttctgga tgtgatcttc tgcgggttgc tcaagggtaa
      961 gtggatcttt cctctttccg cagtgatcac gactctctct ttgaccatta tactcagctt
     1021 ctcttccgga taccatttca tatggttgcc cgttctcatt gccatcgctt ccaaatatat
     1081 cttcactttg aatggaagac acatttacaa tccttcttta ttcgcaattg tattcagtct
     1141 gattttcgca gacggatgga tcagcattgc acccgcctat cagtggaccg gaagcgcaga
     1201 aactgcctgg ctcatgtcga ttacgattct taccgcagga gtcacgattt tcggatacag
     1261 aggaagcagg aaatggctgg cattgtcctt tctcacattc tttgccattc aacttctgat
     1321 tcgtagctac atcattcgct atcatctacc tgccagtgcc ctgttcatag gcacaattac
     1381 ttctccatct ttttatttgt ttacattctt tatgattacg gatccagcca caagtccacc
     1441 cgataaacga actcaaattc tcgttggttt tttcctggcc atatttgacc ttgcgtacca
     1501 cttaaagttt agcctattta ctttctttta cgcggccttc acactcgcga ccatccgcta
     1561 tgttgtggca gcctggaaaa ctcgagcgtg gagtggggcg ctacgaagcc atattcctgc
     1621 tgctattctg actttctttt ttgccattcc cgcattcgcg atgtataaat cgggacaatc
     1681 caatgtgcgt ggttccgcgc ctccgggcct t
//
LOCUS       DAORFF010000695         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_61866_length_1708_cov_3.796733, whole genome shotgun sequence.
ACCESSION   DAORFF010000695 DAORFF010000000
VERSION     DAORFF010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61866_length_1708_cov_3.796733"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PKK76_19245"
     CDS             <1..310
                     /locus_tag="PKK76_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70672.1"
                     /translation="EIYTFVNFLFTLGFASYFFFMSPTMLLGAKLFYGGFVALSLLTL
                     GGIFESKRWAHILEIHRLFIGALMIALYGGDIGFLAAIPIMMAVFFYLQVFRTEMKKA
                     "
     gene            307..>1708
                     /locus_tag="PKK76_19250"
     CDS             307..>1708
                     /locus_tag="PKK76_19250"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNL70673.1"
                     /translation="MILASPGHSEVLLEIEGSTGPVRILFDAWLSDFAFGDFMQRNPR
                     VDLGSIGKLDAVYISHSHCDHLDPYTLTALYQKQTPVLVVPETIEFLVPLLSKFLPGA
                     SIEVLRHNKTSNICGLSWTGFAFAVPYLTNEEDVMPLVIRSKKESVFFEADIALPDSQ
                     EAHGAVDRAMGETERVFVSTRNELEALYASYDAKTPQERKQKLTAYRRRRKDELYWEY
                     ARYAELELPCPFRPGSLKLITGQGMIFPVDLSREFLPLSNPFPLADVTALEVQAARET
                     GYDLKLMTLEAGEQIDSRTIKKSKSQIKVEQTRLTQDPAAPAPAPRPQGPLFDDLRDH
                     SLQKQRILHILNNRFVPHMAYHREEPLKALLLARGSYTIRILYGTSSSHRHEDYTIRF
                     ADMTFRELKEQNKQEGSAGRTKAKPDEIYWANDLEDFLNGRQDQFSTTLHNFFEGTAI
                     RLWTMLGLPFLNNDLIE"
BASE COUNT          441 a          446 c          404 g          417 t
ORIGIN      
        1 tgaaatctat acatttgtga atttcctttt cacactcgga ttcgcgtcct acttcttctt
       61 catgagtcca accatgcttc tgggcgcgaa gctattctac ggcggatttg tggcgctgtc
      121 tcttttgact ctgggcggca tttttgagtc aaagagatgg gcacatatac ttgagattca
      181 tagactattc attggagcac tgatgatcgc cctttacggc ggagacattg gattcctggc
      241 cgcaattcca atcatgatgg cagtgttctt ttacctgcag gtatttcgca ctgaaatgaa
      301 aaaagcgtga ttctggcttc tcccgggcat tcggaagtcc ttctagaaat agagggatct
      361 accggcccgg ttagaatcct gttcgatgcg tggctttcgg atttcgcctt tggcgatttc
      421 atgcagcgca accctcgtgt ggacctcggt agcatcggaa aattggacgc ggtttatatt
      481 tcgcactctc attgcgacca tctggacccg tacactttaa ctgcactcta ccagaaacag
      541 acgcccgttc ttgtcgtgcc cgaaacaatc gagtttctgg tgcctcttct ttctaaattt
      601 ctacccggcg catccattga agtcctcaga cacaacaaaa cgtcgaacat ttgcggactg
      661 tcctggactg gatttgcatt tgcggttcct tatctcacca acgaagaaga cgtgatgcct
      721 ctggtgattc gaagtaagaa agaatccgta tttttcgagg cagacattgc gcttccggac
      781 tcgcaggagg cacacggagc tgtggatcga gcgatgggag aaaccgaacg cgtatttgta
      841 tccacgagga atgaactgga agcactctac gcctcgtatg atgcaaagac tccgcaggaa
      901 agaaaacaga aactcactgc ctatagaaga aggcgaaagg acgaacttta ctgggaatac
      961 gcacgttatg cggaattgga gttgccctgt ccgttcaggc ctggaagcct gaaactgatc
     1021 accggtcagg gaatgatttt ccccgtagat ctctcacgcg aatttctccc tctttctaac
     1081 cccttccccc tggcagatgt aacggcactc gaagtccagg cagcgcgcga aactggatat
     1141 gacctgaaat tgatgactct cgaagccggg gagcagattg actcacgcac gatcaagaaa
     1201 tcaaaatcgc agatcaaagt ggaacagacc aggctcaccc aggacccggc cgcaccggct
     1261 cccgccccgc ggccacaggg accgttgttc gacgatctgc gagatcattc tttgcaaaag
     1321 caaaggattc ttcacatact caacaacaga tttgttcctc acatggcata ccacagagag
     1381 gaaccgctca aggctttgct tttggcccgc ggatcctaca ccattcgcat tctatacgga
     1441 acttcctctt cgcatcgcca tgaagattac acgatccgat tcgcggacat gacgttcagg
     1501 gaactaaagg aacagaacaa acaagaaggg tccgccgggc gaactaaagc aaagccagat
     1561 gaaatctact gggccaacga tctagaagac ttcttgaatg gaagacagga tcaattctcc
     1621 acaactctac acaacttctt cgaaggaact gccatccgac tctggaccat gctggggttg
     1681 cctttcttga acaatgacct gattgaag
//
LOCUS       DAORFF010000696         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_61943_length_1707_cov_3.095642, whole genome shotgun sequence.
ACCESSION   DAORFF010000696 DAORFF010000000
VERSION     DAORFF010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61943_length_1707_cov_3.095642"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1546)
                     /locus_tag="PKK76_19255"
     CDS             complement(<1..1546)
                     /locus_tag="PKK76_19255"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNL70674.1"
                     /translation="MRRLGLILFVFGISALMAQDPAGPAQDEMKTSETARPAASDSKQ
                     ATTTPVSQNNAQATYSKVPTGPDGKPLPLSMEQIVKMVLENNNLVRIQQLEILKSDTD
                     IMKDESKYAPKIGASYEGYEKLDKSSASTIFSGDKVNQDKYTAYMNKLFSSGTYFQVE
                     ASDTRFDSNAGESFALQGTTFAKLAQPPLHTGALTLTLRQELLKNAFGYSQRRLNDIY
                     RNKSVIQREELTQQLAQLVVKTMVDYWSLAIAEENVRTFDLLLSNTRNIRDITIGKAN
                     LGLAEGFEINQWSALTAQAESQQAGAILERNSRRRELLRTLNLDPTLELSGAATLEDK
                     IPTDLDEARDIAYAKEHRPDIRNVRLQMENATLASEIADNSMLPSMYVGGKYSSRDQG
                     RHTITAFNEVPHATYPESGIEFRVEMPLWDDGARVDARNARISLKQMAIQQKQLDRQV
                     EDEIRDNLDRIKTSFDAMKKAESGVEQMTYFYTGLLVRYRQGRFTAVAVKNALDSLVQ
                     AKFALTQ"
     gene            complement(1528..>1707)
                     /locus_tag="PKK76_19260"
     CDS             complement(1528..>1707)
                     /locus_tag="PKK76_19260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70675.1"
                     /translation="ITTEGLKRDMDFLTEAGVKRAILFRLGGLDSEFASILCGNKKIA
                     LPGSEEKQGICADWD"
BASE COUNT          343 a          418 c          448 g          498 t
ORIGIN      
        1 cctgagtgag tgcgaacttt gcctgaacca gagagtcgag tgcgtttttt accgcaacag
       61 ctgtgaatct tccctggcga tatcgcacaa gcaggccagt gtagaaatac gtcatctgct
      121 ctacgcctga ttctgctttc ttcatggcat caaaggatgt cttgatgcga tccaggttgt
      181 ctctgatttc gtcttccacc tgacgatcga gctgcttttg ctggatggcc atttgcttca
      241 ggctgatccg cgcattgcgt gcatccacgc gcgcaccatc gtcccagaga ggcatttcca
      301 ctctaaattc gattccactt tctgggtaag tggcgtgagg gacttcattg aatgcagtga
      361 tcgtgtgtct gccctggtcg cggctggaat atttcccgcc aacatacatg gagggcagca
      421 tggaattgtc tgcaatctcg cttgccagag ttgcgttttc catttgaaga cgaacgttgc
      481 gaatgtcagg gcgatgctct tttgcgtagg caatgtcgcg cgcttcatcc agatccgttg
      541 ggatcttgtc ttccagagtg gctgcaccgc tcagttccag agttggatcc aggttcagtg
      601 tgcgaagtag ttctcttctg cggctgtttc tttctagaat cgcgcctgct tgctggcttt
      661 cagcctgtgc ggtcagggcg ctccactggt tgatctcgaa tccttctgcc aggccaagat
      721 tcgctttgcc gattgtgata tcgcgaatgt ttctcgtgtt gctcaatagc aggtcgaacg
      781 tgcgaacatt ttcttctgca atggcaagag accagtagtc caccatggtt ttgaccacga
      841 gctgagcgag ctgttgagtc agttcttcgc gctggattac agatttgttt ctatagatgt
      901 cattgagtct gcgctggctg tatccaaatg cattcttcag aagttcctgg cgcagagtga
      961 gagtcagagc tccagtatgc agaggaggtt gagcgagctt cgcaaaagtg gttccctgca
     1021 gtgcgaaaga ttctcccgca ttggaatcaa agcgagtgtc ggatgcttcc acctggaagt
     1081 aagtaccgct cgagaacagc ttgttcatgt acgcggtgta cttgtcctgg tttactttgt
     1141 ctccactgaa gatggtggat gcagaagact tatcgagttt ctcatagcct tcgtaggacg
     1201 cgccaatttt gggcgcatac ttggactcat ctttcatgat gtccgtatca gatttaagaa
     1261 tttcaagctg ctggatgcga accagattat tgttttcgag gaccatcttc acaatctgtt
     1321 ccatggaaag aggcagaggt tttccgtccg gtccagtggg gactttgctg tacgtcgcct
     1381 gcgcgttgtt ctgcgaaacg ggagtggtgg ttgcctgctt tgaatcggag gcggccggtc
     1441 gagcagtttc gctcgtcttc atttcatcct gggctggacc cgccgggtcc tgggccatca
     1501 aggcagatat gccgaacacg aatagaatca gtcccaatcg gcgcatatgc cttgtttttc
     1561 ttcactgccc ggcagtgcta tttttttatt tccgcaaagg atacttgcaa attctgaatc
     1621 gagtccaccg aggcggaata aaattgctcg ttttacccct gcttctgtga ggaaatccat
     1681 atctcgcttt agaccctcag ttgttat
//
LOCUS       DAORFF010000697         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_62049_length_1706_cov_2.321018, whole genome shotgun sequence.
ACCESSION   DAORFF010000697 DAORFF010000000
VERSION     DAORFF010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62049_length_1706_cov_2.321018"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1085
                     /locus_tag="PKK76_19265"
     CDS             36..1085
                     /locus_tag="PKK76_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNL70676.1"
                     /translation="MRSWEQSILAPERSILDALKQIDATGIQLVLVAGPAGKLEGVLT
                     DGDIRRAILKGQSLDIAARDVMNSNPVTVRPDTSHEEMLSIMRRGVLHHLPVVDADRR
                     IVGLATLDDLIGAVEKPNRVVLMAGGQGKRLRPLTEDTPKPLLAVGGKPILENILENF
                     AEQGFRRFYVSVNYLAHTISDYFGDGTKWGVKIEYLHEKQSLGTAGALSLLPEVPEEP
                     ILVMNGDLLTRANFSAMLKFHEENKAAATMAVKEYDFQVPYGVVRIEGHEIRGIEEKP
                     VHKFFVNAGIYTISPAALKLIPSGQFFDMPTLFDKLRTAGQSTSAYPLREYWLDIGGL
                     EDFRRANGEFGETFS"
     gene            1082..>1706
                     /locus_tag="PKK76_19270"
     CDS             1082..>1706
                     /locus_tag="PKK76_19270"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNL70677.1"
                     /translation="MIGGKKVLALIPARGGSRGIPRKNIKILGGRPLIGWTIEAARQA
                     AFLDMIVVSSEDEEILTVAKSLGAQALKRPVELATDEASGMDPVRHAIDCFPEIEILL
                     LLQPTSPLRTSGDIDGFMQAFIASGCQSAVGLSPAPSPPEWMYRLDRDGKMKAILGGK
                     VPARRQDLEPAYVINGALYAASSAHLRAGKGFSESGAFGYVMPADRSI"
BASE COUNT          399 a          404 c          482 g          421 t
ORIGIN      
        1 gttggaaatc cagcgcgtgt gatttgaggt aagatttgag aagttgggaa caaagtattc
       61 tggctccgga gcgatcgatt ctcgatgcgc tcaagcagat tgatgctacg gggatccagc
      121 tcgtactggt ggcagggcct gccgggaagc tggaaggtgt tcttacggac ggagatattc
      181 gacgtgcgat tttgaaaggt cagagtcttg atattgcagc gcgagacgtg atgaattcaa
      241 atcccgtgac cgttcgtcca gatacttcgc acgaggaaat gctttcgatc atgcgaaggg
      301 gtgtgctcca tcatttgcct gtagtggatg cggaccgtcg cattgttggg ctggctacac
      361 tggatgattt gattggcgca gttgagaaac ccaatcgagt tgtccttatg gcgggcggtc
      421 aggggaagcg acttcgccct ctgacagaag atacacccaa acctctcctg gccgtcggtg
      481 gaaagcctat ccttgaaaac attttagaaa atttcgcaga acagggcttc cgcagattct
      541 atgtatctgt aaactatctc gcgcacacca tttctgatta ttttggggat ggcacgaaat
      601 ggggcgtcaa aattgaatat ctgcacgaaa agcaaagcct tggtacggca ggagcgctct
      661 cccttcttcc cgaagtgccg gaggagccta ttctggtcat gaacggtgat cttctaacgc
      721 gtgcgaattt tagcgccatg ctaaagtttc acgaagagaa caaagccgcg gcgacaatgg
      781 ccgtgaagga atacgatttt caggtgccct atggagtggt ccgaattgaa ggtcacgaaa
      841 ttcggggcat tgaggaaaag cccgttcaca agttttttgt aaatgctgga atctacacga
      901 tttcccccgc tgcactaaaa ctcattccct cgggtcaatt ctttgatatg ccaacactgt
      961 ttgacaaact caggacggca ggccaatcca catccgcata tccactgcgt gaatactggc
     1021 tggatattgg agggctcgaa gatttccgcc gtgcgaacgg agagtttggc gagacatttt
     1081 cgtgatcggc ggcaagaagg ttctggccct gattccggcg cgcggaggat ccaggggcat
     1141 ccccagaaaa aacatcaaga ttctgggcgg acggcccctg attggatgga ccatcgaagc
     1201 ggcccgtcag gcggcattcc tggacatgat tgttgtttcg tcggaagatg aagagatact
     1261 cacggtggct aaatctcttg gcgctcaagc tctgaaacgc ccggtcgagc tggcaacgga
     1321 tgaagcttcg ggcatggatc cggtaaggca tgccatcgat tgttttccag aaatcgagat
     1381 tcttttgctt ctccagccaa catcgccact tcgcacgtca ggcgacattg atggatttat
     1441 gcaggctttt attgcctctg gttgtcaaag cgctgttggg ctttcgcctg caccttctcc
     1501 tcctgaatgg atgtaccgcc tggacagaga tggaaaaatg aaagcaattc ttggagggaa
     1561 ggtcccggct cgtcgtcagg atctcgaacc ggcgtatgtg atcaatggtg ctctgtacgc
     1621 tgcgagctct gctcatctgc gcgcagggaa aggcttctct gaatcgggcg cgtttggata
     1681 cgtgatgcct gcagatcgat ccattg
//
LOCUS       DAORFF010000698         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_62096_length_1705_cov_3.427273, whole genome shotgun sequence.
ACCESSION   DAORFF010000698 DAORFF010000000
VERSION     DAORFF010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62096_length_1705_cov_3.427273"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(330..1169)
                     /locus_tag="PKK76_19275"
     CDS             complement(330..1169)
                     /locus_tag="PKK76_19275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70678.1"
                     /translation="MIARICLLLALTPLTQCLTMQNIQAARGAPTDTRYKILGASVSD
                     EFAFLDVEGWQMHNTCSSDLDSSVHMPRGQNIPFTQCVSLKDGRAVDEEKCTKRKDFQ
                     VLEPWEKCCSNLLQISRNYYADLSSLNRTAKEVALSEYEKHRLEDISEVKVAPDLSVI
                     QIKEASGRLTTLYGQKSGDGMAFWPGALANNPRTPATVNLPIARRRTLQINGAKFLYP
                     EGDPGPDKFLRTTILTPQRPRPELWPFVPLMMAIDIVTLPFRILSEIGKFIVSPNKCY
                     PLF"
     gene            complement(1192..>1705)
                     /locus_tag="PKK76_19280"
     CDS             complement(1192..>1705)
                     /locus_tag="PKK76_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNL70679.1"
                     /translation="VTDFSEKLESETPASEYYFCCIGTTIKKAGSQEAFRKVDYDLVI
                     NSARAAFAKGARIALVVSALGSDKNSSIFYNRVKGDMEEALQQIGFRTVYVFRPSLLT
                     GDRAEMRPGEKVGQAIGFLAGPLFLGPMKRYKPIAGKTVAAAMVRLAKAQGTGVHFIE
                     SEMIAKVASA"
BASE COUNT          398 a          410 c          437 g          460 t
ORIGIN      
        1 gcaacggcgg cgcacagtag gatcagaaag atttttgtat tgaaaggctt ttccatctca
       61 ggggcgcgcg gtatcctctt gcgaagattc gttgttcttt tgctgttcgg tcaagctcaa
      121 ttgcgaccca tgggcccgga ttttaacttt agaattttgc ttacttaagc acttaactta
      181 tgtgtttgga tcagtacgtt ctaaaactca agaaggagag atcccgtgat caaagtaatg
      241 ttgaagcggt gaaaagatcg agaaacagcc gtggttttgg gttatagatc gcctaacggt
      301 aacccgtcag acgatcttgc cttcgtcatt cagaaaagag gataacactt gttaggactt
      361 acgataaatt tcccaatttc cgaaagaatg cgaaatggca acgtgactat gtcgatagcc
      421 atcatgagcg gcacaaatgg ccatagttcc ggacgcggtc gttgcggcgt cagtatggtt
      481 gtccgtaaga atttgtctgg ccctggatct ccttctggat acaaaaactt ggccccattg
      541 atctgaaggg ttctacgcct ggcaataggc aggttcactg tcgccggcgt gcgcggattg
      601 ttcgcaagtg ctccaggcca gaaagccatt ccatccccac ttttttgacc gtatagagtg
      661 gtgagtcgcc cggaagcttc cttgatttga atgacagata aatctggagc taccttgact
      721 tcagagatat cttccagtcg atgtttttcg tattctgaaa gagccacctc ttttgccgta
      781 cggttcaggc tggaaagatc tgcgtagtag ttccggctga tctgcaatag gtttgagcag
      841 catttttccc agggttccag gacttgaaaa tcttttcgtt tcgtgcactt ctcttcgtcc
      901 accgcgcgcc cgtctttcaa cgaaacgcat tgggtaaaag gaatattctg gccgcgcggc
      961 atgtgaacgg atgaatccag atcagaggag caggtattgt gcatctgcca gccctctaca
     1021 tcgaggaatg cgaattcatc ggaaacgctc gcgcctagta tcttgtaacg cgtgtcagtc
     1081 ggggcaccgc gggctgcctg gatgttttgc atggtcaggc actgagtcag gggagtcagt
     1141 gctagcagta aacaaatgcg cgcgatcaca ggctcaatcg cttactcgtt gttaggcgga
     1201 ggccaccttt gcaatcattt cggattctat gaaatgaacg ccggttcctt gcgctttcgc
     1261 aaggcgcacc atggctgcgg ccactgtctt tcccgcgatt ggcttgtagc gcttcatggg
     1321 tccaagaaat agcgggcctg caaggaatcc gatggcctgt cctacttttt ccccgggacg
     1381 catttctgct ctatctcctg taaggaggga tggtctgaag acatagactg tgcgaaatcc
     1441 aatttgctgg agcgcttctt ccatgtctcc tttgactcga ttatagaaga tgctcgagtt
     1501 tttgtctgaa ccaagagcgg aaacaaccag agcaattcgc gcacctttcg caaatgcggc
     1561 gcgcgcagaa ttgatgacca gatcatagtc taccttacgg aatgcttcct gggatcctgc
     1621 cttcttgatc gtagtgccaa tgcagcaaaa atagtactca gaggccggag tctccgattc
     1681 cagcttttct gaaaagtctg tgacc
//
LOCUS       DAORFF010000699         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_63789_length_1682_cov_2.695759, whole genome shotgun sequence.
ACCESSION   DAORFF010000699 DAORFF010000000
VERSION     DAORFF010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63789_length_1682_cov_2.695759"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PKK76_19285"
     CDS             <1..890
                     /locus_tag="PKK76_19285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70680.1"
                     /translation="DDRLRTKIQADYGVSENKIDAALVEFKETDATVRVVSMLFSVFP
                     FIPQYEYYFSLKGAVRRVARREDADLFEKAKQLANSEDVLKALKVASVVDKADGGLGI
                     YTGLKNAYDAAKNKDRKRTFEADPQQAADAALKAVALSYMVAALYPGSVADKVRLFLE
                     TPAGQEIAAYYAGVEIALPFTDNLIEASGNWMGNLLESAGGAESRFGEFAGSGPMDQA
                     RAILASLREHIDSTLTRVKPYLDPLREKVSTIAPTALNIADSTTGAVATALDVLPAWR
                     FLGARLAAEACVVRAMKGI"
     gene            860..>1682
                     /locus_tag="PKK76_19290"
     CDS             860..>1682
                     /locus_tag="PKK76_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNL70681.1"
                     /translation="MRGSRHERHLKRLFGAGLRREHFPFLLSGTPHSVDWFEAISENY
                     MDSFGRPRQVLRRMRENFPIALHGVSMSIGQTDARREEYLRKLRILADEIDPFLVSDH
                     LCFTGAHGNLHDLLPLPYTEEAALVVSRNLKEAQDSLGRQIAIENVSCYLRYKSSEMT
                     EWEFLVQCASRAGARILLDVNNVYVNARNHGFDGREFINSIPVDLVAQMHLAGHTDTG
                     DFLFDTHSDHVSEDVWKLFELAATRFKDAPVLIEWDESIPEFSELEAEVNRARKVA"
BASE COUNT          445 a          428 c          436 g          373 t
ORIGIN      
        1 ctgacgacag gcttcgcaca aagatccagg cggactacgg agtaagcgaa aacaaaattg
       61 atgcggctct cgttgaattc aaagaaacag acgcaactgt gcgagtcgta agcatgcttt
      121 tctctgtatt tccattcatt ccacaatatg aatattattt cagcctgaag ggtgccgtgc
      181 gaagagtcgc acgcagagaa gatgcagatc tttttgagaa agcaaaacaa cttgcgaatt
      241 cagaagatgt gctcaaagca ctgaaggtag catctgtagt agacaaagcg gacggcggcc
      301 ttggaattta caccggactc aaaaacgcct acgacgcagc caagaacaaa gatcgcaaaa
      361 gaacgttcga agcggaccct cagcaagcag cagacgccgc tctcaaggca gtcgctcttt
      421 cttatatggt tgccgctctc tatcctggaa gcgtggcaga caaagtgcgc ctgttcctcg
      481 aaactcctgc aggacaggag attgcagcct actatgcagg agtggaaatt gcactgcctt
      541 tcacggacaa cctcattgaa gcatccggaa actggatggg caatcttctt gagtctgcgg
      601 gtggagcaga aagcagattt ggagaattcg caggaagcgg acccatggac caggcgcgag
      661 ccatccttgc ctctcttcgc gaacacatcg attccactct gaccagagtg aagccttacc
      721 tggatcctct acgcgaaaag gtgtctacca tcgcgccaac agcgctcaac attgccgatt
      781 caaccacggg tgcagtcgcc accgctcttg atgtattgcc ggcgtggaga ttcctgggtg
      841 cgaggcttgc agcagaagcg tgcgtggttc gcgccatgaa aggcatctga aaaggttatt
      901 cggagcggga cttcgccgcg agcatttccc gttcctgctt tccggcacac ctcattctgt
      961 agactggttt gaggcgattt cagaaaacta catggactcc ttcgggagac ccaggcaagt
     1021 acttcgccgc atgcgagaaa actttcccat tgcacttcat ggcgtatcca tgtcgatcgg
     1081 gcaaaccgac gcacgccggg aagaatattt aagaaaattg agaatcctcg cagatgaaat
     1141 cgatccattt ctggtttcag atcatctgtg cttcaccgga gctcacggaa acctgcacga
     1201 ccttctgcct ctgccgtata cagaagaagc agccctggtg gtatcgcgaa atctcaaaga
     1261 agcgcaggat agcctgggac gccagatcgc cattgaaaat gtttcgtgtt acttaagata
     1321 caaatcatcc gaaatgactg agtgggaatt cctggtccag tgcgcttctc gcgcaggcgc
     1381 gcgcatcctt ctggatgtaa acaatgtgta cgtaaacgcg cgcaaccacg gctttgatgg
     1441 cagggagttc ataaattcca ttccggtgga tcttgtagca cagatgcatc tcgccggcca
     1501 cacggataca ggagatttcc tgtttgatac tcactcggat catgtaagcg aagatgtgtg
     1561 gaaacttttt gaactggcgg ccaccagatt caaagacgcg ccggtgctga tcgaatggga
     1621 tgagagcatt ccagaatttt ctgagctgga agcagaagta aatcgcgcac gcaaggtcgc
     1681 tc
//
LOCUS       DAORFF010000700         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_63791_length_1682_cov_2.684081, whole genome shotgun sequence.
ACCESSION   DAORFF010000700 DAORFF010000000
VERSION     DAORFF010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63791_length_1682_cov_2.684081"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKK76_19295"
     CDS             complement(<1..573)
                     /locus_tag="PKK76_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002753935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNL70682.1"
                     /translation="MADRDYYEILGVARTATKEEIKKAYRKLAIQFHPDKNKGDKAAE
                     EKFKEATEAYEVISDDQKRAAYDQYGKAGVGQGGGPGFGYKAYTDFSDIFGDIGDIFS
                     EFFGGQGGGFAGGGRVRGARRGSDLRYNVEISLEEAASGKEIKIEIPRQEACDDCGGS
                     GAKKGTQASVCPLCQGAGQVRRTQGFFSVTT"
     gene            complement(586..>1682)
                     /locus_tag="PKK76_19300"
     CDS             complement(586..>1682)
                     /locus_tag="PKK76_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017858734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNL70683.1"
                     /translation="IPFITADASGPRHMNMTLSRAKFDQLTKSLVERTRMPCENAIKD
                     AGLSAKEIDEVILVGGSTRIPAVQELVKQLFGKEPNRSVNPDEVVAVGAAIQGGVLAG
                     EVTDVLLLDVTPLSLGIETLGGVFTRLIERNTTIPTRKSQIFSTAADNQTAVSVHVMQ
                     GEREMAKDNRTLARFDLLGIPPAPRGVPQIEVTFDIDANGIVHVSAKDLGTGKEHKIR
                     VETTSGLTEDEIKKMVREAQENEARDKEQRKLVEAKNEADSLTYSLERALQDAGDKVN
                     PADKAAAEDLIKRTREALESGDAERIFQARDEISKGSMDLGQKIYAASQQPGAEGAGP
                     GAQPGGQPGGEERGEKVVDADYTVVDEDKK"
BASE COUNT          332 a          474 c          397 g          479 t
ORIGIN      
        1 agtggtgaca gagaaaaaac cctgagtgcg gcggacctga cctgctccct ggcagagcgg
       61 acatacagat gcctgggttc ctttctttgc gccgcttcct ccgcaatcgt cgcatgcttc
      121 ctggcgcgga atttcaattt tgatttcttt gccggatgct gcttcttcca gactgatttc
      181 aacattgtat ctgagatcag atccgcggcg agctccgcga actcttccgc cgcctgcaaa
      241 accgccgccc tggccgccaa agaattctga aaagatatct ccgatatcac caaagatatc
      301 ggagaagtct gtgtaagctt tgtaaccgaa tccaggtccg ccgccctggc ctactccagc
      361 ttttccatat tgatcatacg ctgcgcgctt ctgatcgtct gaaatcactt cgtaggcttc
      421 tgtggcttcc ttgaatttct cttcggcagc tttgtcgcct ttgttcttgt ccggatgaaa
      481 ctggatcgcg agctttctat aggctttctt gatctcttct ttcgtcgccg tccgggctac
      541 gcccagaatt tcatagtagt cgcgatccgc cattgttcct ccgaattact tcttgtcttc
      601 gtctactaca gtgtagtctg cgtcgacaac cttctctccg cgctcttcgc ctccaggctg
      661 cccgcctggc tgagcaccag gacctgcacc ttctgctcca ggctgctggg aagcagcgta
      721 gattttctgg ccaagatcca tggatccttt tgaaatctcg tctcttgcct ggaagattct
      781 ttcagcgtct ccagattcga gtgcttcgcg agtgcgtttg attagatctt ccgcagccgc
      841 tttgtctgca ggattcactt tgtcgcctgc atcctggagc gcgcgctcca gagaatatgt
      901 cagagagtct gcttcattct tcgcttctac cagcttgcgt tgttctttat cgcgagcttc
      961 gttctcctgc gcttcccgaa ccatcttttt gatttcgtct tccgtaagac cgctggttgt
     1021 ttccacgcga atcttatgct ctttgcctgt tccgagatct tttgcagaca catgcacgat
     1081 gccgtttgca tcaatgtcaa aggtcacttc aatctgcggc acaccgcgtg gtgcaggtgg
     1141 aatgccaagg aggtcgaaac gcgcgagcgt tctgttgtct tttgccatct ctctttcgcc
     1201 ctgcattaca tgcacggaca cagcagtctg gttgtcagcc gcagtagaga agatctgaga
     1261 cttgcgagtc gggatagtag tgtttctctc tatgaggcga gtgaacactc cacccagagt
     1321 ttcaatgccc agagaaagcg gagtaacatc gagtaaaagc acatctgtta cttctcctgc
     1381 gagtacccca ccctggattg ctgcaccaac agccactact tcatccgggt tcacagagcg
     1441 atttggctct tttccaaaaa gctgctttac cagttcctga acagcaggga tacgagtgga
     1501 gccgcccaca agaatcactt cgtcaatttc cttcgcagag aggcctgcat ccttgattgc
     1561 gttttcgcaa ggcatgcgag ttctttctac aagagacttg gtgagctgat caaacttcgc
     1621 gcggctcaga gtcatgttca tatgacgagg accgcttgca tctgcagtga tgaatggaat
     1681 gt
//
LOCUS       DAORFF010000701         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_64779_length_1668_cov_4.482951, whole genome shotgun sequence.
ACCESSION   DAORFF010000701 DAORFF010000000
VERSION     DAORFF010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64779_length_1668_cov_4.482951"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKK76_19305"
     CDS             complement(<1..299)
                     /locus_tag="PKK76_19305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70684.1"
                     /translation="MSSFRFVAIALVFVSQTSITPGPKKKEPEQLCRTYATEFEERPA
                     HTISTCQFDEAALEYRCTKSSGDFKTRVYASARDFVMEGIHRERAYAIRTRFRSDG"
     gene            487..957
                     /locus_tag="PKK76_19310"
     CDS             487..957
                     /locus_tag="PKK76_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNL70685.1"
                     /translation="MKNRQKPDPVQLKSHIGYHLRVISNAVSHSFARKLAATDVTVAE
                     CVILREMYAGNQKTSPGTVAEITGLTRGAVSKLVDRLLNKGLVTRTESSRDRRFQEIQ
                     LTPRAARLVPQLARIADENDESFFSILSQGEKKALVRTLVKLATIHRLNAHPIE"
     gene            971..1384
                     /locus_tag="PKK76_19315"
     CDS             971..1384
                     /locus_tag="PKK76_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HNL70686.1"
                     /translation="MSRLTEKLSKAQQHAMSIRPKVGGFPVLAEVLRECGVEMNRWSL
                     PSCQSIYLMKEGSVVQQGTPLVTGTHEIPKFDRDALIAALRRDQRGESNFPEFLQSAW
                     NAGVTGYDVDFSARKITYYGASGENYLEEYPAVQL"
BASE COUNT          448 a          419 c          409 g          392 t
ORIGIN      
        1 ccatcgctgc ggaatctggt tcttatcgca taggctcgct ctctgtgaat cccttccatt
       61 acgaaatcgc gcgcgctagc atagaccctg gtcttgaaat cgccactgga tttggtgcat
      121 ctatactcga gggcagcttc atcaaactga catgtgctga tcgtgtgtgc tggacgttct
      181 tcaaactcag tcgcatacgt tcggcacaat tgctctggct cctttttctt cgggcccggt
      241 gtaatcgatg tctgcgaaac aaacaccaga gcaatcgcta caaatctgaa tgaggacatt
      301 gcatactccg cctgaagcct tttgttggcg ggctgtctga aaatccattg aacaaccatc
      361 atcccgagag ttctccctgt gtggcacttc gcaagccttt ttgattgaat ggatctgaag
      421 gccagcccca tttgaatcct tgacaactat ttcctaggaa actattatgg acacggaaat
      481 cgacgaatga agaacaggca aaaacctgac ccggttcaat tgaagtctca cattggctat
      541 cacctgagag tgatctcgaa tgctgtttcg cattcattcg cacgcaagct ggcagccacg
      601 gacgtaacag tagcagagtg cgtgattctg cgagaaatgt atgcgggaaa tcagaagact
      661 tctcctggaa cggtcgcgga gatcacaggc cttacgcgcg gagctgtctc caaactggtg
      721 gatcgactcc tgaacaaagg ccttgtgacg cgcaccgaat cttccagaga taggcgcttc
      781 caggaaatcc agcttacacc aagggccgcc cggctcgtgc cacagctggc gcgcatcgca
      841 gatgagaacg atgagagctt cttttcaatc ctctcgcagg gcgaaaaaaa agctctggtc
      901 cgaacgcttg tcaagctcgc aacaattcac agactgaacg cccatccaat cgaatagcaa
      961 ggagtattac atgagtcggc tcactgaaaa actaagcaaa gcacaacaac atgcgatgtc
     1021 gattcgccca aaggtaggag gatttcccgt actcgcggaa gttcttcgcg agtgcggtgt
     1081 agaaatgaac cgatggtctt tgccatcctg ccaatccatt tacctgatga aagaaggttc
     1141 agtcgtgcag caaggtactc ccctggttac aggaacgcat gaaatcccaa aatttgatcg
     1201 cgacgcattg attgcagctc tcaggagaga tcaaagggga gaaagcaact ttcccgaatt
     1261 tctacagagt gcatggaacg caggagttac tggctatgat gtcgattttt ccgcacgcaa
     1321 aatcacctat tacggcgcaa gcggcgaaaa ctatctggaa gaatacccgg ctgttcaatt
     1381 gtgaagcgcc gctctcaaaa ctgaaaagaa tatgcaacac gaagctgatc agaaggcgcg
     1441 atgtctgatt gcaatgattg gtggcgaaag accagcgctt ctgccacatt ggtaagatag
     1501 atcgcaccaa aaaaacccag agctagattc agctggtttg cgcgcgaggc aagtagattg
     1561 cgtttctgcc gaagctgtat agagcggcgg ttagttgatc tgttccgctt ttctgaattc
     1621 cagtccaggt cttgttgaga ataatggttc tcccaaacaa tccgaaga
//
LOCUS       DAORFF010000702         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_65149_length_1663_cov_3.424751, whole genome shotgun sequence.
ACCESSION   DAORFF010000702 DAORFF010000000
VERSION     DAORFF010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65149_length_1663_cov_3.424751"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PKK76_19320"
     CDS             complement(<1..653)
                     /locus_tag="PKK76_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70687.1"
                     /translation="MNPSRRGLAQRAVHQTRIALTLLCACALSFCAQVPQIPEGETDQ
                     AFMTGLLTLLYLDSGNCARSYKVGTNSGQLYCSRAPRSICNYWEGFFYLSPSPIYGKI
                     FVSKATQDRYRADWGFITSDYSTCAASSAAATNLSYPGFKLQTNVQQNNALTIFTHNV
                     VDNCDSLGNSNLSKLATKEQYQFLVSPRGVLAYQARFLGQTTCLDSIPLSAWEKQTA"
     gene            complement(650..>1663)
                     /locus_tag="PKK76_19325"
     CDS             complement(650..>1663)
                     /locus_tag="PKK76_19325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70688.1"
                     /translation="FNVPANLEQISVEAGNPNLKMEESDHAAAGVQQTLGPLWLVKLE
                     AYKNTFDQVVVADSYVVQPFALRTNRRDLLEKQAELSKQPFEARKLSYSNDGTGWSKG
                     VEFYLKKSRPPGKNGFFGWLSYTWSITKRNNHQPRLTQTDSQRLNSDNNGRTPIAYQE
                     FGKNALIYYNTGETYLVLDNDREELYDPDRTHVGSLVVNYKFNPEWQLGGRWKYSTGL
                     PRTPVIGVQDLNLAILGRLTFIPKYSDFYNSERYPAVHQLDLRLDHFTNYEWGYANWY
                     VELINVYAHRNVEGENFDFLYPYAKGSNPAYSYESTYIQTPIGGGRLLLLPLINIGLE
                     MKF"
BASE COUNT          373 a          393 c          453 g          444 t
ORIGIN      
        1 atcgcagtct gcttctccca ggcgctcaaa ggaatgctgt cgagacaggt agtctgtccc
       61 agaaacctgg cctggtatgc gagcactcca cgcggagaaa ccagaaactg atactgttcc
      121 ttcgtagcca gcttgctgag attggagttc cccaatgaat cacaattgtc tacaacattg
      181 tgcgtgaata tagtgagtgc attgttctgc tgcacgttgg tctgtagttt gaagcctgga
      241 taggaaaggt tcgtggccgc agcagaggaa gcggcgcatg tagaatagtc ggaagtgatg
      301 aagccccagt ccgcacggta gcgatcctgc gttgctttgc tcacgaatat tttcccgtag
      361 atgggagaag gggatagata gaaaaaacct tcccagtagt tgcagatgga tctgggagcg
      421 cggctgcaat agagctggcc tgaatttgtg cctaccttgt aacttcgtgc gcaattgcca
      481 gagtccaggt agagcaaggt cagaagtccg gtcatgaatg cctgatcagt ttctccctct
      541 ggaatttgag gcacctgcgc acaaaacgaa agagcgcagg cgcacagcag ggtgagcgcg
      601 attcttgtct gatgcactgc gcgctgtgcc agtccgcgac gcgagggatt caaaacttca
      661 tctccagacc aatgttgatc aaaggcagaa gcaagagtct gcctccaccg atgggcgttt
      721 ggatgtacgt cgattcataa ctgtacgcag gattcgatcc cttcgcgtaa gggtagagga
      781 aatcgaagtt ttcgccttct acgtttctgt gtgcgtatac gttgatcaac tctacatacc
      841 agttcgcata accccactca tagttggtga agtggtcaag acgtaaatcg agttgatgca
      901 cggccggata tctttccgag ttataaaaat cagaatactt tgggatgaaa gtcagccgac
      961 ccagaatcgc caggttgaga tcctggactc caatgacggg cgtgcgcggc agaccggtgg
     1021 agtacttcca cctgccgccg agctgccact ctggattgaa tttgtagttc acgaccaggc
     1081 ttcctacgtg cgttctatcc gggtcgtaga gttcttcgcg gtcattgtcc aggaccaggt
     1141 acgtttctcc tgtgttgtaa tagatgagtg catttttgcc aaattcctga taggcgatgg
     1201 gagttcttcc gttgttgtct gaattcagtc tctgagaatc ggtttgggtg aggcgaggtt
     1261 gatggttgtt tctttttgtg atagaccagg tgtagctgag ccatccaaag aatccgttct
     1321 tgccgggggg ccgtgatttc tttaggtaga attctacccc tttgctccat cctgtgccat
     1381 cgttcgagta gctgagtttt cgcgcttcga agggttgctt cgaaagttcc gcttgttttt
     1441 ccagcagatc tctgcggttg gtcctgagag caaaaggttg tactacgtaa gaatctgcga
     1501 caaccacctg gtcgaatgtg ttcttgtatg cctcaagctt tacgagccag agcggtccaa
     1561 gagtctgctg aactccggct gccgcgtgat ctgattcttc cattttcaaa tttggattcc
     1621 ccgcttccac gctgatctgc tctagattgg cagggacgtt gaa
//
LOCUS       DAORFF010000703         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_65246_length_1662_cov_2.576851, whole genome shotgun sequence.
ACCESSION   DAORFF010000703 DAORFF010000000
VERSION     DAORFF010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65246_length_1662_cov_2.576851"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PKK76_19330"
     CDS             <1..401
                     /locus_tag="PKK76_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70689.1"
                     /translation="VFIRFIREVQHDRIQKGSELRWLYRASRNLAIDYYRKDRKNTPW
                     TDDRIAATGEGEGKIVLGSELVSRMFDVARKCGPDYETLLHLILETRESQSSMAAILK
                     CSERTVRRRTETLFGALERGLPDWRDYLET"
     gene            666..1136
                     /locus_tag="PKK76_19335"
     CDS             666..1136
                     /locus_tag="PKK76_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70690.1"
                     /translation="MLLAAAGFHFSQINHSWNGFRPMGQETVRTGSEASEITLLSGFS
                     TRGRIHLKQDTELSRHGSGHYELHQGQAEVHVHPQGSTVTLWANGIELASQDGVFTIV
                     TGGEENPQGSVTLTVSRGSVECCQNGENFPILPGEKITLVKRDADAQVLAYESE"
     gene            1253..>1662
                     /locus_tag="PKK76_19340"
     CDS             1253..>1662
                     /locus_tag="PKK76_19340"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNL70691.1"
                     /translation="MGAKNKSVLDIGCGQGVYSRALAEAGARVTGIDASPSLIEAARR
                     YQSKVPVKYECENAEDLRAFADQSFDQVSAILCIQNMRNLDRVTSECARVLRPNGRML
                     WVLNHPCFRIPRQTSWGFDEGKKMQYRRIDMYMSP"
BASE COUNT          400 a          412 c          462 g          388 t
ORIGIN      
        1 aggtgttcat tcgtttcatt cgcgaagtgc agcacgatcg gattcaaaag ggttcggagc
       61 tacgatggct gtaccgtgct tctcgtaatc ttgcaatcga ctactatcgt aaggatcgaa
      121 agaacacccc ctggacggat gaccgaatcg cggcaacagg cgaaggagaa gggaagatcg
      181 tgcttggttc tgagcttgtt tccaggatgt ttgatgttgc ccgaaaatgc ggaccggact
      241 atgaaaccct tctacatttg attctggaga cacgcgagtc tcaatcgtcc atggctgcta
      301 ttttgaaatg ctcagaacga acggttcgtc gtaggacaga gacccttttt ggagccctgg
      361 aaaggggact gccagactgg cgggattacc tcgagacatg attttttttg gccccccgga
      421 ggtggaatgt gcgtaaggag agtgtgaaag acgatggcag tctgagtcag gttttgcacc
      481 gacgattcat cgactcgatt cccgggcggg cgactccttc ccttaaccgg aacagcaccc
      541 aggaaaccag tactccggat cttcctgcgg cgccgaattg gatctttgag caggcgtttg
      601 cgcgcgctac gtccccccgg acgcgcgcga accatacgtt ctatccgggt ttggcggcag
      661 ccagtttgct cctggctgcc gcaggctttc acttttccca gatcaatcat agctggaacg
      721 gctttcgtcc tatgggtcag gaaacggtgc gaactggctc tgaggccagc gagatcactc
      781 ttctatccgg gttttctacc cgtggacgca ttcatctgaa gcaagacacg gaactttctc
      841 gccatgggtc cggacactac gagctgcacc aggggcaggc ggaagtccac gttcatcccc
      901 agggaagcac tgtaactctc tgggccaacg gaatcgaact ggcaagtcag gatggagtgt
      961 tcacgattgt caccggtgga gaagagaatc ctcaaggaag cgtgacactt accgtatctc
     1021 gtggtagcgt agaatgttgc cagaacgggg aaaactttcc aattttgccc ggggaaaaaa
     1081 tcacgcttgt aaaacgcgac gccgacgccc aggttctcgc atacgaaagc gaataacaca
     1141 ggaacggtct gatacttcct gggagaagtc ttcagactgg tatgacggcc ttgtaggcaa
     1201 gagtggttcg gatttacatc gcgaaatcat actccctcgc gcgctcgcgc tgatgggcgc
     1261 aaaaaataaa tcggtgcttg atataggttg tggtcaggga gtatacagtc gggctcttgc
     1321 ggaagccgga gcgcgcgtta caggcataga cgcgtctcct tctctgattg aggccgcacg
     1381 ccgttaccaa tcaaaagtcc ctgtaaagta tgaatgcgaa aatgcagaag atcttcgcgc
     1441 attcgcggat cagtctttcg atcaggtgag cgcgattctt tgcattcaaa atatgagaaa
     1501 tcttgatcgc gttacatccg aatgtgcgcg cgtactgagg cccaatggaa ggatgctatg
     1561 ggtcttgaat catccttgct ttcgcattcc cagacagaca tcctggggat ttgacgaagg
     1621 aaagaaaatg caatatcgca ggattgatat gtacatgtct cc
//
LOCUS       DAORFF010000704         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_67257_length_1636_cov_3.163188, whole genome shotgun sequence.
ACCESSION   DAORFF010000704 DAORFF010000000
VERSION     DAORFF010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67257_length_1636_cov_3.163188"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PKK76_19345"
     CDS             <1..928
                     /locus_tag="PKK76_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70692.1"
                     /translation="SMPFPNFTRQRLLRAVCGFAVWKGELDWLYSLALTAGATDPVIF
                     LALSSALLEGRSTLASFFDGRLKQRMPLMDDLLHGIRVPYDEQEPDESAGFLNYFYPG
                     KSRKLGDFRKLGAFREASEAALGWMLLGGHFVKFEKASAFCQAESGSKTNLRQWFAAA
                     NGKKSSNVEGDSTWNLDSSPGIKEGNTPVGTRGRMAAESRAGTYFQARLILARQAIAS
                     RRWMDADSYLKHTLPWHPYVLHLQAQAHIGLGELGLAAACYRKIVKRHPDRAEFCENY
                     GRILSSMGDEAGSARALGRAAEIRSSAKRVYS"
     gene            954..>1636
                     /locus_tag="PKK76_19350"
     CDS             954..>1636
                     /locus_tag="PKK76_19350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70693.1"
                     /translation="MSAYTNEQLKEIDKILKFIKQMEGIVRTSPSREQVERVRKELKK
                     FREKLKEMIPGIDAMTVNVDELRTKLGLTAVIPTVQETAAAKSQDDGESGDILDRFPI
                     QKASPHCTDPDVNLMATMLLVLQKDYWPAISEQHCKLDFSCANERDAIRVLLENAVRS
                     LKVLAETIEEYATADKQDFREQLLKMKNKQTRIFLFETNDVMKGVREFLKKLVDDLDH
                     GGGAVMNRD"
BASE COUNT          420 a          396 c          456 g          364 t
ORIGIN      
        1 gagtatgccg tttcctaact tcactcgcca gagattgctt cgcgctgtgt gcggctttgc
       61 agtatggaaa ggagaactgg actggctgta ttctctcgct ttgactgcag gtgccacgga
      121 tccagtgatt ttccttgcac tctcttctgc gcttctcgaa ggcaggtcca cacttgccag
      181 tttctttgat ggcagattga agcaaagaat gcctttgatg gacgatttgc ttcatggaat
      241 tcgcgtgcct tatgatgagc aagagccgga tgaatccgct ggattcttaa actactttta
      301 cccgggcaaa tcgcggaagt taggcgactt tagaaagctc ggtgctttta gagaagcatc
      361 ggaagcggct cttggctgga tgcttctggg cggtcatttt gtgaagtttg aaaaagcaag
      421 cgccttctgc caggctgagt ctggaagtaa gacgaatctg cggcaatggt ttgcggcagc
      481 caatggaaag aaatccagca atgtggaggg cgattctacc tggaatctgg attcttcgcc
      541 tggcatcaag gaaggaaata ctccagtggg aacgcgtggc aggatggccg cagaaagcag
      601 agcgggaacc tactttcagg caaggttgat tctggccaga caggccatcg cctcgcgcag
      661 atggatggat gccgattctt atttgaaaca tactctcccc tggcatcctt acgtacttca
      721 cctgcaggcg caggctcaca ttggactggg agagctgggc ctggccgccg cctgttatcg
      781 caaaattgta aaaaggcatc cagatcgagc ggaattctgc gaaaactatg gacgcattct
      841 ttcctcgatg ggagacgaag caggctcagc ccgggcgctc gggcgcgctg ccgaaattcg
      901 gtcgtcggca aaaagagttt actcataggg ttttgtccct tacaaggtgc acagtgagcg
      961 cttacacaaa cgaacagcta aaagagatcg ataagatcct gaaattcatc aaacagatgg
     1021 aaggcatcgt gcgtacttca ccaagccgcg agcaggtgga gagagtgcgc aaggagctca
     1081 agaagttccg cgaaaagctc aaagaaatga ttcccggcat tgatgcaatg acagtcaatg
     1141 tggacgagct tcgcacaaaa ctgggcctca ccgcagtcat tccaaccgtt caagaaacag
     1201 ccgcggccaa atcccaggac gacggagaga gcggagacat tctggatcgg tttcctatcc
     1261 agaaagcgag tcctcattgc acagacccgg acgtgaactt gatggcaacc atgcttctag
     1321 ttcttcagaa agactactgg cctgccatat ccgaacagca ctgcaaactt gatttttcct
     1381 gcgccaatga gcgcgacgcg atccgtgtgc tattggaaaa cgccgtgcga agtctcaagg
     1441 tgcttgctga gactatagaa gaatatgcca ctgcggacaa gcaggacttt cgcgagcagc
     1501 tcctcaagat gaagaacaag cagacccgga tcttcctgtt tgaaacgaac gacgtgatga
     1561 agggtgtgcg agaattcctt aagaagctgg tggatgacct ggaccacggg ggcggagccg
     1621 tcatgaaccg ggatga
//
LOCUS       DAORFF010000705         1622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_68383_length_1622_cov_4.171027, whole genome shotgun sequence.
ACCESSION   DAORFF010000705 DAORFF010000000
VERSION     DAORFF010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68383_length_1622_cov_4.171027"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PKK76_19355"
     CDS             complement(<1..887)
                     /locus_tag="PKK76_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017858734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNL70694.1"
                     /translation="MAKDKIIGIDLGTTNSCVAIMEGGEPIVIQNAEGSRTTPSIVGF
                     TDKEARLVGQVAKNQAITNPTNTVRSIKRFMGRRFSEVQDEMKVVPYKVIRSGTDGIK
                     VETDQGSFTAQEVSAIILQKMKKTAEEYLGHPVTKAVITVPAYFNDEQRQATKDAGRI
                     AGLEVERIINEPTAAALAYGLDKKTKNMKIAVYDLGGGTFDVSILELGEGVFEVKSTN
                     GDTHLGGDDFDQAIMQWMMDEFKKESGIDISKDRMALQRLKEAAEKAKIELSGTLQSQ
                     INIPFITADASGPRHMNMTL"
     gene            complement(950..1561)
                     /gene="grpE"
                     /locus_tag="PKK76_19360"
     CDS             complement(950..1561)
                     /gene="grpE"
                     /locus_tag="PKK76_19360"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNL70695.1"
                     /translation="MENKVMEETQKEETLEGVDVAAGSPDQKEQPASESAGVNLELEK
                     AKAEAMDMRDSWNRERAEFQNYKKRMIHEMGRARSQAVKSFVSGLLPVIDNLNQVLLA
                     KSEDPAVKNFVIGVEMIRSEFLKVLNKENIQSFRPVDQAFDPMRMEAVALDESESVDS
                     PKVVEVYEDGYSLTIDGQEEILRPARVRVLKPAKKKEEANENA"
BASE COUNT          353 a          428 c          334 g          507 t
ORIGIN      
        1 agagtcatgt tcatatgacg aggaccgctt gcatctgcag tgatgaatgg aatgttgatc
       61 tgtgactgga gagttccaga cagttcaatc tttgcttttt cagcagcctc tttcaatcgc
      121 tgcagagcca ttctatcttt tgagatatcg attccgctct cttttttgaa ttcgtccatc
      181 atccattgca tgatggcctg atcaaagtcg tcaccgccca ggtgagtgtc cccgtttgta
      241 gactttactt cgaatacacc ttctccgagc tctagaatgg atacgtcgaa cgttccacca
      301 cccaggtcat acactgcgat cttcatgttc ttggtcttct tatccagacc atatgcgaga
      361 gcggctgcag taggttcgtt gatgatacgt tctacttcca ggcccgcaat tcttcctgca
      421 tccttggtag cctgacgctg ctcatcattg aagtaagctg gaaccgtgat cacagccttg
      481 gtgactggat gacccaggta ttcctctgca gttttcttca tcttctgtag aatgatcgca
      541 gaaacttcct gagcagtgaa agagccctgg tctgtttcta ctttgattcc gtctgttcca
      601 gagcgaatta ctttgtatgg aacaaccttc atttcatcct gaacttcaga gaagcgtctt
      661 cccatgaagc gcttgatgga acgtacagtg ttagtcgggt tagtgatggc ctgatttttt
      721 gccacctgac ccaccagcct tgcctctttg tcagtaaaac cgacgataga cggcgttgta
      781 cgagatcctt ccgcgttttg aatgacgatt ggttcaccgc cttccatgat tgcgacgcat
      841 gagttggtag tacccaggtc gattccaatg attttgtctt ttgccatttc ctttctcctc
      901 tcaatgattg cgcgattgct cgcttatatt aacaaaattt ctaaactgtt caagcatttt
      961 catttgcctc ttctttcttc ttcgcaggct tgagcactct gactcgcgcc ggacgaagaa
     1021 tctcttcctg accgtctatc gttaaagaat aaccatcttc gtatacttcg accacttttg
     1081 gagaatctac actttcgctc tcatcaagcg ccactgcttc catgcgcatt ggatcaaatg
     1141 cctgatccac agggcgaaag gactgaatgt tctctttgtt cagcactttg agaaattcgc
     1201 ttcggatcat ttctacgccg atcacgaaat tcttcacagc cggatcttct gatttcgcaa
     1261 gaagaacctg gttcaagttg tcaatcactg gaagaagccc gctaacgaaa ctcttcacag
     1321 cctgtgaacg agcgcggccc atctcatgaa tcatgcgctt tttataattt tgaaactcag
     1381 cgcgctcgcg attccaggaa tcgcgcatat ccattgcttc cgcttttgcc ttttcaagct
     1441 ctagattgac gcctgcactt tcagacgctg gctgctcttt ttgatccggc gaaccggcgg
     1501 caacgtccac accttcgaga gtttcttctt tttgcgtttc ttccatcact ttattctcca
     1561 tatcaattgc tcatcctggt aatcatgctg gacaggagtc tgcggatgta ttccaccacg
     1621 ct
//
LOCUS       DAORFF010000706         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_69891_length_1604_cov_4.000000, whole genome shotgun sequence.
ACCESSION   DAORFF010000706 DAORFF010000000
VERSION     DAORFF010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69891_length_1604_cov_4.000000"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PKK76_19365"
     CDS             <1..474
                     /locus_tag="PKK76_19365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNL70696.1"
                     /translation="YLALKSYDPAVKSFRAVSDSRSASADQRKEAYRGLAYCLSQSGR
                     SEDQELARSEMNKAVRMDPDDPDMQLMRAGMLMKTGSMVDREKAIEVLQALTAGDLSS
                     DKASQAYNLLGLAFHKNGEYSRALKAFDSAIQLNPQNREAYENQRASANAYERQK"
     gene            518..1468
                     /locus_tag="PKK76_19370"
     CDS             518..1468
                     /locus_tag="PKK76_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNL70697.1"
                     /translation="MRSTVLRPGFRVRAFAPAAIAISIYSANCASFLLNRSLSFERGR
                     ADLEVKTIPVGKFHFVYTEGGKGEAIVLIHGFAGEKDHWTRMARYLTDKYRVIAPDLP
                     GHGENDRLPEENYSIPEQARRLHDFVHQIGLKRFHLVGNSMGGAISTYYASEYPEDVI
                     TLALFDSAGVKSPVKSELATYLEKGENPLVVKTRDDFDRLTAFTFVDPPYIPGVIKSY
                     FTEKAIANKEFNEKIFSDISKDRGGVEGRLSKIQIRTLILWGDTDRVIHPSSTEVFHK
                     GIKNSEVVIMKACGHGPMIERPQETADHYLKFLNKSASAK"
BASE COUNT          449 a          370 c          411 g          374 t
ORIGIN      
        1 tatctggctc tcaagagtta tgatcctgct gtgaaatcgt ttcgcgctgt ttctgattcc
       61 agaagtgcaa gtgcagatca aagaaaagaa gcataccgcg gtcttgcgta ttgtttgtct
      121 caatcaggca gatcggaaga tcaagaactg gccagatcgg aaatgaacaa agcagtccgc
      181 atggatccag acgatcctga tatgcagctc atgcgtgctg gaatgctcat gaaaacagga
      241 tcgatggtgg accgggagaa agcaattgaa gtgctccagg ctctcactgc aggagatctg
      301 tcgagcgaca aagcttctca ggcatacaat ctgcttggcc ttgcgtttca caagaacggg
      361 gaatactctc gggcactcaa agcatttgac tctgccatcc agctgaaccc tcagaacaga
      421 gaagcatacg aaaatcagcg cgcaagcgcc aatgcgtacg aaaggcagaa gtaaagaaaa
      481 gaaatcgcca tagcgagcgt tcatgacaac gagggtcatg agatctactg ttctccgtcc
      541 aggctttcga gtacgcgcgt ttgctccggc tgccattgca atttctatct acagcgcgaa
      601 ctgcgcttcg ttcttactca atcgtagtct atcttttgaa cgcggccgcg cagatctcga
      661 agtcaaaaca atcccggttg gcaaattcca tttcgtatac acggaaggag gaaagggaga
      721 ggcgattgtt ctgattcacg gatttgccgg agagaaagac cactggactc gcatggcgcg
      781 ctaccttaca gacaagtaca gagtcatcgc gcctgatctt cctggacacg gagaaaatga
      841 caggttgcca gaagagaact actctattcc ggagcaggcc aggagattgc acgatttcgt
      901 tcatcagatt ggcctgaagc gatttcacct ggtcggaaat tcaatgggtg gtgcgatttc
      961 cacctactat gcatccgagt atcctgagga cgtaatcact cttgcgctgt ttgattctgc
     1021 gggcgtcaag agtccggtga agagcgagct ggccacgtat ctagagaaag gagaaaatcc
     1081 actggtggtg aagaccagag acgattttga tcgtttgact gcctttactt ttgtggatcc
     1141 accttatatc cctggtgtaa tcaagagtta tttcacagaa aaagcgattg cgaacaaaga
     1201 attcaacgag aagatttttt cagacatcag caaggatcgc ggaggggtgg agggcaggct
     1261 ttctaagatt caaattcgca ctttgattct ctggggagac acggacagag tgatccatcc
     1321 ttcttccaca gaagtgtttc acaaaggaat caagaattct gaagtagtga tcatgaaggc
     1381 atgtggacac ggccccatga ttgaaaggcc gcaggagact gcggatcact acttaaagtt
     1441 tctaaacaaa tctgccagcg ccaaatgatt caagaagccc agagtccaag agacactgtg
     1501 ctgtagagat agtagctgct tgcaatcagc gccagatgat agacatcgtt gtggagtttg
     1561 tattcgcgca gccatttcac tcgcgcaaga cccatccata gagc
//
LOCUS       DAORFF010000707         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_70138_length_1601_cov_4.305304, whole genome shotgun sequence.
ACCESSION   DAORFF010000707 DAORFF010000000
VERSION     DAORFF010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70138_length_1601_cov_4.305304"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PKK76_19375"
     CDS             <1..637
                     /locus_tag="PKK76_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70698.1"
                     /translation="IVSAYQAFSFRNNLERDTLKKGRIVLSTGDEATFDAAKNREGSA
                     RLILDGDPGTCANLYFPSNHPEGTHLIAELALTHDPGSPPQSRKLRGIKFLNGCTDTG
                     NDRIKKLEISILLRRANDPDVEKIIPEPVEMLRETVEIPDRSVVWIPFDVAPFPASPG
                     YPDHVSLVIAKIRILETYHPEQHRAGIRELYYVDLDASSRRERIWSPGESL"
     gene            634..1395
                     /gene="panB"
                     /locus_tag="PKK76_19380"
     CDS             634..1395
                     /gene="panB"
                     /locus_tag="PKK76_19380"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNL70699.1"
                     /translation="MTLKYPSHWLERKGGPKISVITAYDYTFARLIDRSQIDAVLVGD
                     SLGMMIQGKPSTLPVTVDEMVYHSSLVRRGTQKFMISDIPFGVIHASFEVACSAAVRL
                     MKEAGADAVKIEGASEPCLNVIRHLTANGIPVMGHLGFLPQSIHTLGGYRIQGKSGPD
                     ALVAQSVQLEKAGAFAMVLELIADSTAAEITKKTSIPTIGIGSGNSTDGQVQVLHDVL
                     GLDPDFRPKHAMRMRDIGSDLISACSEFHNSIQIK"
     gene            complement(1415..>1601)
                     /locus_tag="PKK76_19385"
     CDS             complement(1415..>1601)
                     /locus_tag="PKK76_19385"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HNL70700.1"
                     /translation="IFLGQMLSAMRKNLHKDSDMMYGGRTEEIFQDFLYDEYAKMMSK
                     SPGFNLSDEIYKQLKGE"
BASE COUNT          428 a          397 c          387 g          389 t
ORIGIN      
        1 gattgtttcc gcctaccagg cattcagctt cagaaacaat ctggaaagag acacgctgaa
       61 gaaagggcga atcgttctat cgacaggaga cgaagccact tttgatgctg cgaaaaacag
      121 agaaggatcc gcgcgcttga tcctggatgg agatccaggc acctgcgcga atttgtactt
      181 tccatccaac catcccgaag gcacccatct gattgcagag ctggcgctga cgcatgatcc
      241 aggaagcccg ccgcagagcc gtaaattgag gggtattaag tttctgaacg gttgtacgga
      301 tacaggcaat gacagaatca agaaacttga aatttctata ttattgagaa gagccaatga
      361 tccggacgta gagaagatca tccccgaacc ggtcgaaatg ctgcgcgaga ctgtggaaat
      421 ccctgatcgc tctgttgtgt ggatcccgtt cgatgtcgcg ccctttcccg cctccccggg
      481 ctatccggac catgtgtcgc tcgtaatcgc caaaattaga attcttgaaa cttatcatcc
      541 agaacaacac agagccggaa tcagagaatt gtattatgtt gacctggatg cgtcttcacg
      601 cagagaaaga atctggagcc cgggagaaag tttatgacac tcaaatatcc gtcccactgg
      661 ctggaacgca agggcgggcc aaagatcagc gtcattacgg catatgacta tacgtttgca
      721 agattgatcg atcgaagcca gatcgatgcg gtgctcgtgg gagattctct tggcatgatg
      781 atccagggta agccctctac tctgcccgta actgtagacg agatggtata ccattcatct
      841 ctcgtgcgaa gaggcactca gaagtttatg atctctgaca ttccattcgg agtcattcat
      901 gcatcctttg aggtcgcatg ctccgccgca gttagactca tgaaggaggc gggtgctgac
      961 gcggtcaaga ttgagggagc atccgaaccc tgcctgaacg ttatccggca tttaactgca
     1021 aatggaatcc cggtcatggg ccatcttggt ttcctgcccc aaagcattca taccctggga
     1081 ggctatcgca tccagggaaa gagcggtcca gatgcactgg tcgcccaatc cgtccaactc
     1141 gaaaaagcag gcgcatttgc catggtactc gagctaatcg cggattccac tgctgcagaa
     1201 attacaaaaa aaacatccat tccaactatt ggaattggtt ccggcaattc tacggatggt
     1261 caggtgcagg tactccacga tgtgctgggg ctcgatcctg attttcgtcc caaacatgca
     1321 atgagaatgc gtgacatcgg tagcgatttg atctccgcct gctccgaatt tcacaattcc
     1381 attcagatca agtgaattgt aaacgtcaaa agagttattc gcctttcaac tgcttataga
     1441 tctcatcaga caggttgaac ccaggagatt tggacatcat ttttgcgtat tcgtcgtata
     1501 gaaaatcctg gaagatttcc tctgtgcgac caccatacat catatcactg tctttgtgta
     1561 agttctttct catcgcagaa agcatctgac caagaaaaat c
//
LOCUS       DAORFF010000708         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_70237_length_1600_cov_3.344984, whole genome shotgun sequence.
ACCESSION   DAORFF010000708 DAORFF010000000
VERSION     DAORFF010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70237_length_1600_cov_3.344984"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="PKK76_19390"
     CDS             <1..1218
                     /locus_tag="PKK76_19390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNL70701.1"
                     /translation="GSRVGPIMRATKKRLRILFICPADVSTAQSFVATVADTGSFDVL
                     VFDSGRQGPFPAQKWPFPTISIYRSEHPANGWHRWIGLPSNRFVRFLIQKRHWISALF
                     LRMVIRVYRPDVVHCLGINPESRILLRALRGISKTRRPPWALTSWSADVYWDRYKPEC
                     LSHLRESFETVGWFLADCKRDLRIAVELGLSPAKLLIQDAIPGVGHVNAENIKRPLRD
                     GPLTILVPKAYDGVYHKPHIMLEALSRLRLRGLTFRLKIIGAQEDVRIWWRLRDPQGE
                     AAAEFFDSLPREEYLEMLRTADILLAPSLTDGTPNALLEAMSTGAFPIFSSHESIREW
                     IQDGRNGLLVDPVDCVDIEQKLIRVFENRALIPAAAELNGQIVKERLGKVRMQELLTQ
                     GYETLANSTGGAG"
     gene            1296..>1600
                     /locus_tag="PKK76_19395"
     CDS             1296..>1600
                     /locus_tag="PKK76_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70702.1"
                     /translation="MERIRQAIGRVEARQVKDASRIADAEFRVYSQWGEDGIIQNLIG
                     RIPGVDPSFIEFGVEDYRESNTRFLLVNNNWRGLILDAAGSQLDTVRRSKTYWQHSLT
                     "
BASE COUNT          349 a          397 c          441 g          413 t
ORIGIN      
        1 ggaagtaggg tcgggccaat catgagagcc acaaagaaga ggttgcgcat cctttttatc
       61 tgccccgcgg acgtttccac cgcgcagagt tttgtcgcaa cagttgcaga tacgggcagc
      121 tttgatgtgc ttgtgtttga ttccggtcgt caggggcctt ttccggctca aaagtggcct
      181 ttcccaacga tttcgatcta tcgatcggag catccagcaa atggctggca tcgttggatc
      241 ggtttgcctt ccaatcgctt tgtgcgattt ttgattcaaa aacgccactg gatctccgcg
      301 cttttcctgc gaatggtcat tcgtgtttat cggccagatg tagtgcattg tcttggaatc
      361 aatccagagt cgcgcatttt gcttcgcgca ttgcgcggca tttcaaagac caggcgacct
      421 ccctgggcgc ttacgtcgtg gagtgcggac gtgtactggg atcgatacaa gccagagtgt
      481 ctgtcacatc ttcgtgaatc ctttgagaca gtgggctggt ttctggccga ttgcaaacgc
      541 gatcttcgca tcgcggtgga gctgggtctt tcgcctgcaa agcttctgat ccaggatgcg
      601 attcccggtg tgggtcatgt gaatgcggag aacataaaac gaccgttacg cgacggtcct
      661 cttaccatcc tggtccccaa ggcgtatgac ggtgtttatc acaaaccgca catcatgctg
      721 gaagctcttt cgcgactccg cctgcgcggc ctgactttta gactgaagat cattggtgcg
      781 caagaagacg ttcgcatctg gtggcgtctt cgcgatccgc aaggagaggc tgcggctgaa
      841 ttttttgatt cactgccgcg cgaagaatat ctggagatgc ttcggaccgc cgatattctc
      901 ctggcaccca gtcttacaga cgggacaccc aatgctttgc ttgaagccat gtccacgggt
      961 gcatttccaa tcttctcttc gcacgaatcg attcgtgaat ggattcaaga cggtcgcaat
     1021 ggattgctag tagatccagt ggactgcgtg gacatcgaac aaaagctcat tcgtgttttt
     1081 gaaaaccgcg ctctcattcc ggctgctgcg gagttgaacg gacagatcgt gaaggagcgc
     1141 ctgggtaagg ttcgcatgca agaattgctc actcagggct atgaaactct cgcaaattct
     1201 acgggtggtg caggatgaag ttccttttgt ttccgataag attgctgcgt tctatcaaag
     1261 agctgttgcg acttgttcct cttatgagcg aagaaatgga acgtattcgc caggccatcg
     1321 gccgtgtaga agcacgtcag gtgaaggatg caagccggat cgcggacgca gagtttcgcg
     1381 tgtattccca atggggcgaa gatggaatca ttcaaaatct gattggccga attccaggtg
     1441 tggatccgtc cttcattgaa tttggcgtgg aagactacag agaatcgaac actcgctttc
     1501 tgcttgtgaa caacaactgg cgaggtctca ttctggacgc tgccggttcg cagctcgaca
     1561 cggtgcgcag aagcaaaaca tactggcagc atagcctgac
//
LOCUS       DAORFF010000709         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_70425_length_1598_cov_3.604666, whole genome shotgun sequence.
ACCESSION   DAORFF010000709 DAORFF010000000
VERSION     DAORFF010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70425_length_1598_cov_3.604666"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKK76_19400"
     CDS             <1..311
                     /locus_tag="PKK76_19400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70703.1"
                     /translation="IASQHFEVSDSEFSTIMDTVRFDLISAAMIFDGKPADFLEKVEE
                     NAIIVQGIDRSDWNAETEEAAHMGILRQYFVEMGLSRKSISAADETWFHGFAASAQAV
                     "
     gene            324..974
                     /locus_tag="PKK76_19405"
     CDS             324..974
                     /locus_tag="PKK76_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70704.1"
                     /translation="MNLFLRILLLLLVSGALGAQSVSAPLERHVPQEDKRRLVFAKER
                     AGEAENIYRYAVELHSEGYKERALELLRDFRIRFPGHERTFFVRMEEGDILREKGEIL
                     DAVNMDLETCRMYPREERAGRVCLRAGKLLRRSGDVDRARSVFKEVADSFPASGVARM
                     ASVELSLMSESQNTPDQPEPAVNEQRNNDEMPTNEQKEPIREEELPGQGVFDAKEP"
     gene            1022..>1598
                     /locus_tag="PKK76_19410"
     CDS             1022..>1598
                     /locus_tag="PKK76_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003006165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNL70705.1"
                     /translation="MSFQMVNFKAGSYVVIEGKREALSFFIIRQGKLKTQKENPVIGE
                     DSSQILGPGDFFGVESAMSGHSRIETVVALSDCSLIQVNNDQFGLLIQKNAPVAMKII
                     RSFSVKLRSFDNTITRLSFRNAVEEDPSHLFVLGEHWMREGKTNHAAYAFQKYLQWNP
                     QGDKLSQAKIHLQSLNKPLKAPVIPETSINRT"
BASE COUNT          411 a          404 c          417 g          366 t
ORIGIN      
        1 aaattgcaag ccagcatttc gaagtttctg actctgagtt cagcacgatc atggacacag
       61 ttcgttttga tttgatttcc gcagccatga tcttcgacgg caagcccgca gatttcctcg
      121 agaaagtaga agaaaacgca atcatcgtcc agggcataga tcgatccgac tggaacgcgg
      181 aaacggaaga agcagctcac atgggcatcc ttcgccagta tttcgtagag atgggtcttt
      241 cgcgcaaatc catcagtgct gcagacgaga catggttcca cggtttcgca gccagcgctc
      301 aggcagtctg atcgagcttt tccatgaacc tctttcttag aattctactg cttctgctgg
      361 tctcgggagc tctgggtgcg cagagcgttt ctgctccact ggagcgtcac gtgccccagg
      421 aagacaaacg caggcttgtg ttcgccaaag agcgggcagg cgaagcagag aatatctatc
      481 gatacgccgt cgagttgcac tcagaaggct acaaagaacg agccctcgaa ctattgcgcg
      541 actttcgcat ccgctttccc ggccatgaga ggacgttctt cgttcgtatg gaagaagggg
      601 atatactgcg cgaaaaagga gagatcctgg acgcagtgaa catggacctg gagacctgtc
      661 gcatgtatcc gcgcgaagag cgcgcgggca gagtctgttt gcgagcagga aagctcctgc
      721 gccggtcagg cgatgtagac cgcgccaggt ctgtttttaa ggaagtcgcg gacagtttcc
      781 ctgcctctgg agttgccaga atggcgtccg tggagctcag tttgatgagc gaatcgcaga
      841 acacgccaga tcagcctgag ccggccgtta atgagcaaag aaataacgac gaaatgccga
      901 caaatgaaca gaaggagcca attcgggaag aggaattacc tggacagggc gtttttgacg
      961 caaaggaacc ttgattttct tgttgtcact agtgccaccg gaaacacccc tgagataagc
     1021 catgtccttt caaatggtca actttaaagc gggctcctat gtggtcatag agggcaaacg
     1081 cgaagctctg agtttcttca tcatccgtca gggcaagctg aagacccaga aagagaaccc
     1141 cgtcattggc gaggacagtt cccagatcct tggtcctggg gatttctttg gcgtggagag
     1201 cgccatgtcc ggccactcca gaattgaaac tgtagtcgca ctgtctgact gctctctcat
     1261 ccaggtaaac aacgatcaat tcggacttct cattcagaag aacgcgcctg ttgcgatgaa
     1321 gatcattcga tctttctctg taaagctcag atcatttgat aacacgatta cgcgtctttc
     1381 atttcgcaac gcagttgagg aagatccttc gcatttattt gttctgggcg aacactggat
     1441 gcgcgaaggc aaaacaaatc acgcagctta cgcgtttcag aagtatctac agtggaatcc
     1501 gcagggcgac aaactcagcc aggccaaaat tcatcttcaa tctctcaata agcctctgaa
     1561 agctccagtc attccggaga catcgatcaa tcgtacgt
//
LOCUS       DAORFF010000710         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_71444_length_1586_cov_3.271718, whole genome shotgun sequence.
ACCESSION   DAORFF010000710 DAORFF010000000
VERSION     DAORFF010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71444_length_1586_cov_3.271718"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PKK76_19415"
     CDS             <1..340
                     /locus_tag="PKK76_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNL70706.1"
                     /translation="NIVEQTLGRGIPEASDHHSTSLCCGAGGAQMWMEEQYDRVNVKR
                     TNQLLETGADTIATACPFCMVMITDGVKAAGMQDQVQVLDVAELVAASMKGGTGAIPH
                     AHGKAEPAHH"
     gene            complement(564..1109)
                     /locus_tag="PKK76_19420"
     CDS             complement(564..1109)
                     /locus_tag="PKK76_19420"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70707.1"
                     /translation="MINQYLARVLFLSVVLLAAQCSSSQTQENKPQERGQVPTTGCIQ
                     GDCTAGDGTFVYNGGDKYLGSFKNGLREGKGTLDYASGDQYIGQFVADKRSGDGTYKF
                     ANGDVYVGQFKDGERAGNGVYTFKEGPVFEGTFANDGKSGNGSLKAGDKLKRCELQDR
                     KIVCEADKAEAPATETKTDKK"
     gene            1169..>1586
                     /locus_tag="PKK76_19425"
     CDS             1169..>1586
                     /locus_tag="PKK76_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004469228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNL70708.1"
                     /translation="MNTPSKKSNRLAKEKSPYLLQHAHNPVDWYSFGEEAFAKAKSED
                     KPILLSIGYATCHWCHVMERESFEDEETANYLNEHFVAIKVDREERPDVDAIYMRALH
                     ATGQHGGWPLNMFLTTDLRPITGGTYFPPKPAYGRPS"
BASE COUNT          392 a          424 c          395 g          375 t
ORIGIN      
        1 caacattgta gaacaaactc tcggccgtgg cattccagaa gcgtccgacc atcactctac
       61 atctctttgc tgcggtgcag gtggcgccca gatgtggatg gaagaacaat acgacagagt
      121 caacgtgaag cgtaccaatc agctcctcga aacaggagca gacacgatcg ccactgcctg
      181 cccattctgc atggtcatga tcacagacgg tgtgaaagca gccggaatgc aggatcaggt
      241 gcaggtgctc gacgtggcag aactggtggc ggccagcatg aagggaggca caggtgccat
      301 ccctcacgct cacggcaaag cggaaccagc tcaccactaa accattcaat tacgaccggg
      361 cggcatttgc cgcccgcgca ctctcccccg gatcgattcc ctcgcaggtt ctctgtctct
      421 gcaggtctct gtaaattcca ctggaagctt ccagaagcgt gagcgcgcac tggctgccag
      481 ggttgcccga gggctgtatt tgatttgttc gtgtgaggat tcggaatgcg gcccgcccag
      541 gttgggcggg ctttctggaa ggattacttc ttgtctgttt ttgtttcagt ggctggagct
      601 tctgctttgt ccgcttcgca tacgatctta cgatcttgca attcacagcg cttcagtttg
      661 tctcctgctt tcagagaacc gttgcctgac ttgccgtcat ttgcaaatgt tccttcgaaa
      721 actggacctt ccttgaaagt atagactccg tttcctgcac gctcgccatc tttgaattga
      781 ccaacgtaga catcaccgtt tgcaaatttg tatgttccgt caccgcttct cttgtctgcg
      841 acaaactggc caatatactg gtcaccggat gcataatcaa gagtgccttt gccttcgcga
      901 agtccgttct tgaaagaacc aaggtattta tctccgccgt tgtagacgaa tgttccatcc
      961 ccggctgtgc aatcaccctg gatgcaacca gtcgttggaa cctggccacg ctcttgaggc
     1021 ttgttttctt gtgtttgaga agaagagcac tgagctgcca gaagaacaac ggatagaaaa
     1081 aggaccctgg ctaaatattg attgatcata gaagtctcct gggtcggaga tgacccggtt
     1141 ctgttcgaat gcaaggaaaa aaaagcgcat gaatacgcct tcaaaaaagt caaatcgcct
     1201 cgcgaaagaa aaaagtccct acctgctcca gcatgcgcac aatccggtgg attggtacag
     1261 cttcggagag gaggctttcg cgaaagctaa atcggaagac aaaccaatct tgctctccat
     1321 tggctacgcg acatgtcact ggtgccacgt aatggaaaga gaatcattcg aagacgaaga
     1381 aactgcgaac tatctcaacg aacatttcgt tgccatcaaa gtagatcgcg aagagagacc
     1441 ggatgtggac gccatttaca tgcgcgcact ccacgcaaca ggacagcacg gtggctggcc
     1501 gctcaatatg tttctaacaa cagatcttcg cccgattacc ggtggtacgt actttccacc
     1561 taagccagca tacggcagac caagtt
//
LOCUS       DAORFF010000711         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_71526_length_1585_cov_4.893464, whole genome shotgun sequence.
ACCESSION   DAORFF010000711 DAORFF010000000
VERSION     DAORFF010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71526_length_1585_cov_4.893464"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PKK76_19430"
     CDS             <1..445
                     /locus_tag="PKK76_19430"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNL70709.1"
                     /translation="GWGMTGAGYIPVERGDRKKAMESLFQAADRIQGGASVIIFPEGT
                     RSENPHEGMLPFKKGAFVLAKKANVTIQPITIWGAHHIMPAKQKVWVQRIYPGLVQVE
                     VHDPISPEESGKMDQEQLSAHVRTVIERPMKVMLETLDDNEKKRG"
     gene            complement(453..>1585)
                     /locus_tag="PKK76_19435"
     CDS             complement(453..>1585)
                     /locus_tag="PKK76_19435"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNL70710.1"
                     /translation="FCSAHTVELAGKRYTVLVNADAMGKSIQGAGGALVLGAVFESIM
                     ERTRSIPSFRDQSPERWLKNAFQELHGIFESFDGSMLVSMVLGLLDDESGLLYYVNVE
                     HPWTVLYRGGKASFIETEHQFRKLGVAGVEGQIRVQTFQMEPDDVLILGSDGRDDILL
                     GTSESGERIINEDEHLFLKMVEAAQGRLPEIRSHLEEQGPLTDDLSLIRVAYRSHEED
                     QAPDREDAMTAYREKARQLMEENKFAEAAVALGDALQLDPRSLKTIRMMVRLFLDQKD
                     YDRAFAQVEDYIYLRPLDTDMIYLASYLCKKLKKLRDAAEFGERVRLRAPDMVRNLLN
                     LSEVYARLGNKDRSAKILREVYKLEPDNKKADKLRKLLETAA"
BASE COUNT          417 a          426 c          379 g          363 t
ORIGIN      
        1 tggctggggc atgacgggag caggatacat tccggtagaa aggggagatc gcaagaaggc
       61 aatggaatct ctcttccagg cggcagacag aatccagggc ggcgcttccg taatcatttt
      121 cccggaagga accagaagcg aaaatccgca cgagggaatg ctccccttta aaaagggcgc
      181 attcgtactg gcaaagaagg caaacgtaac catccagccc attaccatct ggggcgcgca
      241 ccacattatg cccgcgaagc aaaaagtatg ggtgcagaga atttatcctg gactggtcca
      301 ggtggaagtg cacgatccca tctctccgga agaatctggc aagatggatc aagagcagct
      361 atcggcacat gtaaggaccg tcattgaaag accaatgaaa gtcatgcttg agacgttaga
      421 cgacaacgaa aagaagcgcg gatagttccg cgctacgccg ctgtttccag aagcttgcgc
      481 aacttgtctg ctttcttgtt atccggctcg agcttgtaca cctctcgcaa tatcttcgca
      541 gagcgatcct tattcccaag cctcgcgtac acttcagaca ggttgagtag attgcgcacc
      601 atgtcaggcg cacgcagccg cactctctct ccaaattctg ccgcatcacg cagcttcttg
      661 agcttcttgc agaggtagct tgccagatag atcatgtctg tatccagagg acgcagataa
      721 atgtagtctt ccacctgcgc gaaagcgcga tcatagtctt tttgatcgag gaacaaccgg
      781 accatcatac ggattgtttt gagactacgc ggatccagct gcagtgcatc tccgagagcg
      841 actgcggcct ccgcgaattt attttcttcc ataagctggc gagctttctc gcgataggca
      901 gtcatagcat cttctctgtc cggtgcctgg tcctcttcat gcgaacgata ggcaacgcga
      961 atcaaagaaa gatcatccgt aagtgggccc tgttcttcca ggtgagagcg aatttcagga
     1021 aggcgaccct gggctgcttc taccatcttg agaaataaat gctcatcttc attgatgatt
     1081 ctttctccgg attcagaagt acccaggaga atgtcgtcgc gtccgtctga accgagaatc
     1141 aaaacgtcgt ctggttccat ctggaatgtc tgtacgcgaa tctgaccttc tacgcccgcc
     1201 acaccaagct tgcgaaactg atgctctgtt tcaatgaagc ttgctttgcc accacgatac
     1261 agaacagtcc agggatgctc cacgttcacg tagtacagca aaccactttc atcatccagt
     1321 aaaccaagca ccatggagac gagcatgctt ccgtcaaaac tttcaaaaat gccatgcaat
     1381 tcctgaaacg cattcttcag ccatctttct ggagattgat cacggaaaga aggaatagaa
     1441 cgagttcgct ccataatgga ttcgaaaact gcccccagta ccagagcacc accggcgccc
     1501 tgaatggatt tccccatagc atctgcgttc accaataccg tataacgttt gccggcaagt
     1561 tccacagtat gcgcactgca gaaat
//
LOCUS       DAORFF010000712         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_72185_length_1578_cov_3.844386, whole genome shotgun sequence.
ACCESSION   DAORFF010000712 DAORFF010000000
VERSION     DAORFF010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72185_length_1578_cov_3.844386"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="PKK76_19440"
     CDS             <1..1030
                     /locus_tag="PKK76_19440"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70711.1"
                     /translation="ISEYRDPDNTKATVPARGSDFRRYGVLLAVLLVILLSKGKLFDG
                     MYERLQWKKDMFAKGLDYRFARLYESKSGVIAIGPDGSVHGGGMYDGFLDYRLKLGGW
                     LLRPYSLSLFHPHPRRVLIVGVSGGAWTAVVNGHPQVESITAVEINKHYRQVMEDEPK
                     MRHLLTSPKLNLVFDDGRRWLNRNPEEHFDAVIMNTTYHFRTYVSNLLSREYMTLVSS
                     HLSPGGVFLFNTTDSKEAMRTALEVFPHCMMILNATICSKEPLEFNAKRWLSVLDSYR
                     INGEQILSGEEGRKTRAWLETLTTEIDQKDALYEREGFRSQKRMLEDCSGARIVTDDN
                     MATEYGDH"
     gene            complement(1043..1116)
                     /locus_tag="PKK76_19445"
     tRNA            complement(1043..1116)
                     /locus_tag="PKK76_19445"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1080..1082),aa:Arg,seq:acg)
     gene            complement(1175..>1578)
                     /locus_tag="PKK76_19450"
     CDS             complement(1175..>1578)
                     /locus_tag="PKK76_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70712.1"
                     /translation="GLSDGSVARARVLMRFRPGHEHYTTLAQVVERGVLFCSSGEPGY
                     DRVMATQAGIVIRLDPDLLATLPDARARMQELVRLKAEGSEFIPRLEKRYGVRVEDLL
                     AQSSQWEADRLMQSKQACWKSFEGGPDTYFY"
BASE COUNT          401 a          401 c          394 g          382 t
ORIGIN      
        1 gatttcagaa tacagagatc cggacaacac gaaagcaacg gtccctgcgc gcggatccga
       61 cttcagacgc tacggagtct tgcttgctgt actacttgtg atccttttgt caaaagggaa
      121 actctttgat ggaatgtacg aacgcctcca gtggaagaag gacatgttcg cgaaaggtct
      181 ggactatcgc ttcgcgcgac tctacgaaag caagagcggc gtaatcgcaa ttggccccga
      241 tggttctgtt catggcggcg gaatgtatga cggttttctg gactaccgct tgaagctggg
      301 aggctggttg ctcagaccct attctctttc tttgtttcat cctcatcccc gtcgcgtttt
      361 gatcgttgga gtgtcaggag gcgcgtggac tgctgttgtc aatggacatc cgcaggtaga
      421 atccatcact gcagtagaaa tcaacaagca ctatcgccag gtgatggaag acgaacctaa
      481 gatgcggcat cttctcacca gccccaaact caatctggtc ttcgacgatg gtcgtcgatg
      541 gctcaaccga aacccggaag aacacttcga cgcagtgatt atgaacacaa cgtatcactt
      601 tcgcacgtac gtgagtaact tactctccag agaatacatg actcttgtga gttcgcatct
      661 atctccaggc ggggttttcc ttttcaatac tacggactca aaagaagcaa tgcgcacagc
      721 gcttgaagta tttcctcatt gcatgatgat tctaaacgcg accatttgca gcaaagagcc
      781 acttgaattc aacgctaagc gatggttgtc tgttctggac agttacagga tcaacgggga
      841 acagattctt tcgggtgaag aaggaagaaa gacacgcgca tggcttgaga ctctgacgac
      901 tgaaattgat cagaaagatg cgctctatga gagagaagga ttcagaagtc aaaaacgaat
      961 gctagaggat tgttctggtg cacgcatcgt cacagacgat aacatggcta cggagtacgg
     1021 agatcattaa gagaaaaaaa tttgcgccca gaaggattcg aacctccgac ctactggttc
     1081 gtagccaggc actctatcca gctgagctat gggcgcgtga gggccatttt tcggacctgc
     1141 gttttagtgt cgaacggttt tcgactgtga gcgcctagta gaagtaagtg tccggaccgc
     1201 cttcaaaaga tttccagcat gcctgcttgc tctgcataag tctgtctgct tcccactgag
     1261 aagactgagc cagcaaatct tctactcgca cgccgtatct cttctcaagc ctgggaatga
     1321 attcgcttcc ttccgctttg aggcgaacca gctcctgcat tctcgctctg gcatccggca
     1381 aggttgcgag cagatcagga tccaggcgaa tgacaatccc agcctgcgtg gccatcacgc
     1441 gatcatatcc aggttctcct gaactgcaaa acaatactcc tctttccaca acctgtgcca
     1501 gggttgtgta atgttcgtgg cccggacgaa aacgcatgag cactcgagct cgtgcaacag
     1561 agccatcgct caatccct
//
LOCUS       DAORFF010000713         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_73031_length_1569_cov_2.968296, whole genome shotgun sequence.
ACCESSION   DAORFF010000713 DAORFF010000000
VERSION     DAORFF010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73031_length_1569_cov_2.968296"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PKK76_19455"
     CDS             <1..887
                     /locus_tag="PKK76_19455"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M17"
                     /protein_id="HNL70713.1"
                     /translation="QLTRKHGVSLKVFGMSDLEKMGCGGIVSVGKGSRIPPRMMILEY
                     KPKNAKNKKPIVLAGKGITFDTGGISLKPAGEMHEMKYDMCGSAIVIHALALAARRKL
                     PVHITGIVAIAENMPGGNAIKPGDVFTAYNGVTVEIQNTDAEGRLVLADILSYACAHL
                     KPGVLFDFATLTGACVVALGHETAGVMTPDDELADRIDRTSRRSLDRTWRLPHWRQYG
                     QGLKSEVADLRNVSGRDGGTITAFRFLSKFVQRSVPWAHFDIAGIAWHSKGYGTRTKG
                     ASGWGLSFLNRYFEDEMR"
     gene            909..>1569
                     /locus_tag="PKK76_19460"
     CDS             909..>1569
                     /locus_tag="PKK76_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70714.1"
                     /translation="MMKHMIPGVILVLASCATTYNQFYLAQPVPSDRASRILITPIFI
                     TKSQIRILSKYGVKWEEAEDEQAFDTNFHFLTELLQRKTNHESELLTKEDRDELMDEL
                     EVSGTPKLAERNEGARNFWNGKHCASCIKKLLDERNAKWLLVPSVMVSEFSGNYYWSG
                     NSYCPDRHWAGLRSQYLLFDTEGILIMDSSQLTPSNFLTGVEPASVGIHISDLVVSRT
                     QG"
BASE COUNT          400 a          354 c          435 g          380 t
ORIGIN      
        1 agcagctcac tcgcaaacat ggagttagcc tgaaagtatt cgggatgtca gacctggaaa
       61 agatgggatg cggcgggatt gtttccgtgg gcaaaggatc cagaattcca ccacgaatga
      121 tgattctaga gtacaaacca aagaatgcaa agaataagaa acccatcgtt ctggcgggaa
      181 aaggcatcac ctttgatacg gggggaattt ctctcaagcc ggccggtgaa atgcatgaaa
      241 tgaaatatga catgtgcgga tcggccatcg tcatccatgc tctggccctg gcagcaaggc
      301 gtaagctccc ggttcacatc actggaattg ttgcgatcgc ggagaatatg cctggtggaa
      361 atgcaatcaa gccaggagat gtattcactg cttacaatgg tgtcaccgtg gagatccaga
      421 atacggacgc ggagggcagg ctcgttcttg cggacattct gtcctacgct tgcgctcatc
      481 taaagccagg ggttctcttt gattttgcaa cgctcacagg tgcgtgtgtc gttgctctcg
      541 ggcatgagac ggcgggggtg atgaccccgg atgatgaact cgcggataga atcgatagaa
      601 cttcgcgcag gtctctagac agaacgtgga gactgcctca ctggagacag tatggccagg
      661 gtttgaaatc agaagtggca gacctgagaa acgtttctgg gagagacggg ggcactatca
      721 ctgcatttcg ttttctatcc aagtttgtgc agagaagtgt tccctgggcc catttcgata
      781 tcgcagggat tgcctggcac tcgaaagggt atggaactcg cacgaagggc gccagcggat
      841 ggggactttc gtttttgaat cgttactttg aagatgagat gagatagcac tggcgagggt
      901 tttctttcat gatgaagcat atgataccgg gcgtgattct agttttagct tcgtgcgcta
      961 caacctacaa tcagttctac ctggctcagc cagtaccttc cgatcgcgca tctcgtatcc
     1021 tgattacacc aatcttcatt acgaagtcac agattcgcat cctctcgaag tatggcgtga
     1081 agtgggagga agcagaggat gagcaagcat tcgataccaa cttccatttt ctgaccgaac
     1141 tcttgcagag aaaaacaaat catgaaagtg aattgctaac aaaagaagat cgcgatgagc
     1201 tgatggacga gctggaggtg agcgggactc cgaagcttgc cgaaaggaat gaaggagccc
     1261 ggaacttctg gaatggaaaa cactgcgcat cctgcattaa gaagctcctc gatgagcgga
     1321 atgcgaaatg gcttctggtt ccttccgtca tggtcagtga attttcaggt aactattatt
     1381 ggagcggcaa ttcctactgt cctgatcgac actgggccgg cttgagaagt cagtatcttc
     1441 tttttgacac ggaagggatt ttgatcatgg attcaagcca gctgactccc tccaattttc
     1501 ttactggagt ggagcccgcg agcgttggca ttcacatttc cgacctggtt gtcagtcgca
     1561 ctcagggcg
//
LOCUS       DAORFF010000714         1553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_74567_length_1553_cov_2.302403, whole genome shotgun sequence.
ACCESSION   DAORFF010000714 DAORFF010000000
VERSION     DAORFF010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74567_length_1553_cov_2.302403"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PKK76_19465"
     CDS             <1..418
                     /locus_tag="PKK76_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70715.1"
                     /translation="VGPVAVAFSTSGGLDKRYTDDDVPSVSRYAPWSGSYTVAAPPVP
                     PLSVDYTVDSDSSFHVMLQTDIPELTIELLDHNRRPIASRVFKHPPRDLSFKIPVPAG
                     KEVRAIHISAGDFHTWGEVSWVGYSFRASTFVEKEP"
     gene            466..>1553
                     /locus_tag="PKK76_19470"
     CDS             466..>1553
                     /locus_tag="PKK76_19470"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNL70716.1"
                     /translation="MKQKHAQFSQFDLSESIQKGIVDAGFSEPSPIQIEAIPLVLKGR
                     DVVAQAQTGTGKTAAFGLPLMSRLLPARSVQILVITPTRELALQVSEELQSLGKHAQA
                     KAVSVYGGASAREQISALKHGANIVAGTPGRLLDLMSSGRMKIEPSAVVLDEADRMLD
                     MGFIEDIEQIFEFLPDARQTLLFSATLPERVQKLAQKILSEPVRIRTQSTETAARIKQ
                     KYFVVSENEKEAALVRLLEFDPPDRAIVFSKTKREADELSVRLTARGIPSRPIHGDLE
                     QNKRERVLSSFRAGTTRLLIATDVAARGLDIPGVSHVFNYHIPYDAESYVHRIGRTGR
                     AGQEGVALTLVSPGEFRSWKRMTRENGGT"
BASE COUNT          389 a          381 c          409 g          374 t
ORIGIN      
        1 cgtggggccg gttgcggttg ctttttcgac atccggcgga ctggacaaac ggtatacaga
       61 cgatgatgtg ccatccgttt cacgttatgc gccatggtct ggttcctaca cggtcgccgc
      121 tccaccggta cctcctttga gtgtagatta tactgtagat tcggattcca gttttcatgt
      181 gatgttgcag acggacatac cagagctgac cattgagctt ctggatcaca atcgtagacc
      241 tatcgccagt cgcgttttca aacacccgcc gcgagatttg agtttcaaga taccggtgcc
      301 cgcaggcaaa gaagtaagag ccattcacat cagcgcggga gattttcata cctggggcga
      361 agtgagctgg gttggttatt cctttcgcgc atctaccttt gttgaaaagg agccatagtt
      421 tctttttccc atatccagga gcggcagttt agaaaaagtg accaggtgaa gcagaaacac
      481 gctcagttct cgcagtttga cctttcagaa tcgatccaga aaggaatcgt ggatgcgggt
      541 ttctcagagc ccagtccaat tcaaatcgaa gccattcctc ttgtcttaaa gggacgcgat
      601 gtggtagcgc aggctcaaac aggaacagga aagacagctg catttggtct tccgctcatg
      661 agcagacttc ttcctgcaag gtccgtacaa attctggtca tcactccgac gcgcgaactc
      721 gcgctgcagg tgagcgaaga gcttcaatct ctgggaaagc atgcgcaagc gaaagcagtt
      781 tctgtatacg gtggtgcatc cgcgagagag caaatatctg cgctcaagca cggagccaat
      841 attgtggctg gaacaccggg cagattgctc gacctgatga gctccggccg catgaaaatc
      901 gaaccgtctg cagtggtttt agatgaagcg gatcgaatgc ttgatatggg attcattgag
      961 gatattgagc agatcttcga atttcttcca gacgcaagac aaacgttatt gttttccgcg
     1021 actcttcctg agcgcgttca gaaactcgct cagaaaattc taagtgagcc tgtgcgcatt
     1081 cgcactcaga gcacggaaac ggctgcgcgc atcaaacaga aatactttgt tgtatctgaa
     1141 aacgaaaaag aagcagctct ggttcgtttg ctggagtttg atccaccgga tcgggccata
     1201 gtatttagca aaacgaaaag agaagcggat gaactttctg tccgcctcac tgcacgcggc
     1261 attccgtcga gacccattca tggggacctg gaacagaaca agagagaaag agtcctttcc
     1321 tcctttcgcg cgggaaccac acgcctcctg atcgcgactg atgtggctgc acgcggtctg
     1381 gatattccgg gcgtgagtca cgttttcaat tatcacattc catacgatgc agaaagctac
     1441 gttcatagaa tcggccgcac aggtcgcgcg ggccaggaag gagtggcttt gactctcgtt
     1501 tctccaggcg aatttcgctc atggaaaaga atgactcgtg aaaatggcgg aac
//
LOCUS       DAORFF010000715         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_75310_length_1545_cov_3.896644, whole genome shotgun sequence.
ACCESSION   DAORFF010000715 DAORFF010000000
VERSION     DAORFF010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75310_length_1545_cov_3.896644"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /gene="truB"
                     /locus_tag="PKK76_19475"
     CDS             complement(<1..798)
                     /gene="truB"
                     /locus_tag="PKK76_19475"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNL70717.1"
                     /translation="MIGGFVPAWKPPGMGSAEIVGRLKRTIHADKVGHTGTLDRFAEG
                     LMLLVVGRSTCLCDFFLHADKRYSAIFSFGKSTETHDPEGSVLEERGEDESISFVSQH
                     LDQIREWIENQIHTTEQTPPLYSALKKQGKRYSDHARAGVKELPPARKVTIYSASLTS
                     HSGYQVTADFSVSGGTYIRSIARDLGEEFQFPVHLAGLRRSAIGSFLLNEDCWRSGEE
                     VRIRPPHEVLTWPRVKASDAEVRSLLFGQTPRLQMPSGSQFAWIVGAD"
     gene            complement(795..1187)
                     /gene="rbfA"
                     /locus_tag="PKK76_19480"
     CDS             complement(795..1187)
                     /gene="rbfA"
                     /locus_tag="PKK76_19480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNL70718.1"
                     /translation="MKEIRQKKLESSLLREMALAIQRRSRTGDGIGLVSVTRVELKTD
                     MSEVIFHVSPFGDPEDNRMTMQTLNRLAGQLQSQISRDLHLRSTPRFSFRVDNSIKEG
                     DRILEKLESTKPPSSVSQESGKDDEAEE"
     gene            complement(1209..>1545)
                     /locus_tag="PKK76_19485"
     CDS             complement(1209..>1545)
                     /locus_tag="PKK76_19485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNL70719.1"
                     /translation="KAAMEGLLEPDKVEEVSGRAEIRTVFKISRMGNIAGCMVVQGKV
                     RKANKVRLIRDGVVIYTGSLRSLRRVKDDVPEVAEGFECGIAIEGYNDIREKDIIESF
                     EIKEIARKL"
BASE COUNT          375 a          405 c          363 g          402 t
ORIGIN      
        1 atctgcaccc acgatccagg cgaactgact tccggatggc atctgtaacc tgggggtttg
       61 tccgaacaac aaactgcgaa cctctgcgtc gcttgctttc acgcgcggcc aggtaagaac
      121 ttcatgagga gggcgaatgc gcacttcttc tccggaacgc cagcaatctt cattgagcaa
      181 aaaagaaccg atggcacttc ttctgagtcc ggccaggtgc acaggaaatt gaaactcttc
      241 tcctagatcg cgcgcgatag aacgaatgta tgtgccgcca gaaacgctga agtctgctgt
      301 gacctggtag cctgagtgcg aagtgaggga tgctgaataa atggttactt ttcgcgcagg
      361 tggaagttcc ttcacaccgg cccgagcatg atccgaatat ctttttccct gtttcttcaa
      421 agcagaatac aatggaggag tctgttctgt tgtgtggatt tgattttcaa tccattcgcg
      481 aatctgatcc agatgttgtg aaacaaagga gatggattca tcttcgcctc tttcttcgag
      541 cacgcttcct tctggatcat gcgtctctgt gctcttgcca aatgaaaaga tggccgaata
      601 acgtttgtcc gcatgcaaaa agaaatcgca taaacacgta gagcgcccga cgacaagaag
      661 catcagacct tccgcaaaac gatcgagagt tccagtgtga cctactttgt ctgcgtgaat
      721 ggttcttttg agtctgccta cgatttcagc agaacccatc cctggcggtt tccaggctgg
      781 aacaaaaccg ccgatcactc ttccgcctcg tcatccttgc cggactcctg cgatacagaa
      841 gacggcggct tcgtactttc taatttttct aaaatgcgat ccccttcctt gatgctattg
      901 tctacgcgaa aggaaaagcg cggagtagaa cgaaggtgaa gatcgcgact gatctgtgat
      961 tgtagctggc ccgccagacg attgagagtc tgcatggtca tgcgattgtc ttccggatcc
     1021 ccgaatggag aaacatgaaa gatgacttct gacatgtctg ttttcaattc aacgcgcgta
     1081 acagaaacaa gacctatccc atctcccgtc cgactgcgtc tttgaatcgc aagagccatc
     1141 tcgcgaagaa gcgatgactc cagtttcttt tggcgaattt ctttcatgaa agaatcgggc
     1201 ccgtttgctt agagcttacg ggcgatttct ttgatctcga acgattcaat gatatccttt
     1261 tcacgaatgt cattgtaacc ttcgatggca atcccgcatt cgaatccttc cgcaacttcg
     1321 ggcacgtcgt ctttgacgcg tcttagactg cgaagagatc cagtgtagat caccacaccg
     1381 tcgcgaatca ggcgcacttt gtttgctttg cggactttgc cctggacaac catgcaacct
     1441 gcaatgttac ccatacgcga aatcttgaag actgtacgaa tttccgcacg gccagaaacc
     1501 tcttccactt tgtccggctc aagaaggcct tccatcgcgg ctttc
//
LOCUS       DAORFF010000716         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_75509_length_1543_cov_3.756048, whole genome shotgun sequence.
ACCESSION   DAORFF010000716 DAORFF010000000
VERSION     DAORFF010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75509_length_1543_cov_3.756048"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="PKK76_19490"
     CDS             complement(<1..1065)
                     /locus_tag="PKK76_19490"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNL70720.1"
                     /translation="MHHEKAGSPIVSALGNGRLQVVRNPEVDYILDLVLDEARRLVQA
                     RQGNFYLFNSISKLEPFLPAAASEQDIALCEDVIQARTSRLIPENPALCVYMTLQEGD
                     LGVLLLREPAYFDQFYEADLALVQNLAATFAILLKSAFIDQRTPEVFLGFKSSLLLLL
                     ENAHLNHKIKESDHQLKSVLEVSNLINSSRELSEMIQTVLYSAKRVIRAESASLFLVD
                     EKTGELYFEIIAGNKKDLKGFRIPRGQGIAGITAATKKSLIVNDAQNDARVFRQVDQV
                     SREITRNILAAPLLIEGEAIGVIEVINTIDRPAFSEHDLEIFESFSDSVAIALQRRRL
                     LDSIQRTNVQLERRLREITSL"
     gene            complement(1067..>1543)
                     /locus_tag="PKK76_19495"
     CDS             complement(1067..>1543)
                     /locus_tag="PKK76_19495"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNL70721.1"
                     /translation="AIASWEKIEQIKPGYRDVRDKLRQYQEFRTDDSVKDFLIMNTTQ
                     FENACRRIIEKMGFQVLHLMLNGEMSVTGVGTDADVKSRNRGQKTLFFISRDMAALPE
                     KIVREFHESMREKGALRGVIMTTGEVMPAALNYATTRPIEIYDSSAVVPFIRTAMN"
BASE COUNT          345 a          378 c          379 g          441 t
ORIGIN      
        1 aagacttgtg atctcgcgga gccttctttc cagctgtacg tttgtccttt gaatgctatc
       61 caggaggcgc cttcgctgta aggcgatcgc caccgagtca gaaaaacttt cgaaaatttc
      121 caggtcgtgc tcagagaaag cgggacggtc gatcgtattg atcacttcaa tgacgccgat
      181 tgcttctcct tctattagaa gaggtgccgc cagaatattg cgagtgattt ctctggatac
      241 ctgatctacc tggcgaaaca ctctcgcgtc attttgcgcg tcgtttacaa tgagagattt
      301 cttcgtggca gcagtgatgc ctgcgatgcc ctgtccgcga gggatgcgaa agcctttcag
      361 gtctttcttg ttgcccgcaa tgatttcgaa atacagttct cccgtttttt catccaccag
      421 gaacagactc gcgctttctg cgcgaatcac tctcttcgca ctgtaaagaa ctgtctggat
      481 catctctgaa agttcgcgcg acgagttgat caagtttgaa acttctagaa cactcttgag
      541 ctggtgatcg gactctttga ttttgtgatt taagtgcgcg ttttcaagga gtaagagcag
      601 gctgcttttg aaccccagaa atacttctgg cgttcgttga tcaatgaatg cgctcttcaa
      661 gagaattgca aatgtagcgg ccagattctg cacaagcgcg agatctgctt catagaactg
      721 atcaaagtac gcgggttcgc gaagcaagag tacgcccaga tctccttctt gcagggtcat
      781 gtaaacgcac agggccggat tctcgggaat cagtctgctt gtgcgtgcct ggatgacgtc
      841 ttcgcacaga gcgatgtcct gttcagatgc ggcagcggga agaaatggct ccagtttgct
      901 gattgagtta aaaagataga aatttccttg tcttgcctgc acgagacgcc tggcttcatc
      961 gagtactaga tccagaatat aatccacttc cggattgcga accacctgaa ggcgtccgtt
     1021 tcccagagcc gaaacaattg gactgcccgc tttttcgtga tgcattttag ttcatcgcag
     1081 tgcggatgaa aggaacaacc gcgctggagt catagatttc gatgggtcgt gtagtcgcgt
     1141 agttgagagc ggccggcatc acttctcctg tagtcatgat cacgccgcga agggcgcctt
     1201 tttcgcgcat agattcgtga aactctctga ctattttttc cgggagcgca gccatatcgc
     1261 gtgatatgaa gaagagagtt ttttgcccgc gattcctgct ttttacgtct gcatctgtgc
     1321 caacgcccgt aacactcatt tctccgttca gcatcaggtg aagcacctgg aagcccatct
     1381 tctcaatgat gcgcctgcac gcattttcaa attgagtcgt gttcatgatc aagaaatcct
     1441 tcacggaatc gtctgttctg aattcttgat attgtctgag tttgtctctg acatcacggt
     1501 agcctggttt gatttgctct atcttttccc agcttgcgat tgc
//
LOCUS       DAORFF010000717         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_75513_length_1543_cov_3.593414, whole genome shotgun sequence.
ACCESSION   DAORFF010000717 DAORFF010000000
VERSION     DAORFF010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75513_length_1543_cov_3.593414"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1543)
                     /gene="carB"
                     /locus_tag="PKK76_19500"
     CDS             complement(<1..>1543)
                     /gene="carB"
                     /locus_tag="PKK76_19500"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNL70722.1"
                     /translation="KRVDTCAGEFEAHTPYLYSTYEEMNESAPTNNQKVIILGGGPNR
                     IGQGIEFDYCCCHASYALQDMGIESIMVNSNPETVSTDYDTSSKLYFEPLTAEDVLHL
                     CELEKPDGVIVQFGGQTPLKLARGLEAAGVRILGTSPDAIDRAEDRDRFSEMIKKLGL
                     LQPENGMAYNLEEAQAVVERIGYPVLVRPSYVLGGRAMALVHDRAGLEKFVKEAEQIN
                     PEHPILIDRFLEEAEELDVDALSDGKEVFTAGILQHIEEAGVHSGDSACVIPPPSLSP
                     AMIEEIKTATRMLALELGVVGLINIQMAIQENRLYIIEVNPRASRTVPFVSKAIGLPL
                     AQAATQLLMGKSISEVIPPAMLSGKTTGFVAVKEVVIPFFRFPGADTILGPEMKSTGE
                     VMGLAPTFEEAYVKAVIGAGEKIPEAGGVFVSVNNIAKQSLATEIKILKDLGYVMYAT
                     EGTAEFLKTKGVDVKVLHKLRDQVSPSALDEMINGNLKLIINIPYSQTTRDDAFLIRQ
                     EASRRG"
BASE COUNT          347 a          383 c          381 g          432 t
ORIGIN      
        1 ccgcgacggc ttgcttcctg gcgaatcaaa aatgcatcgt ctctggttgt ctgcgaataa
       61 ggaatattga tgatgagctt gaggttccca ttgatcattt catcgagcgc actcggagag
      121 acctgatcgc gcaacttgtg cagcactttt acatccacac cttttgtttt gagaaactca
      181 gctgtgcctt ctgttgcata catcacgtag cccaggtctt tgaggatttt aatttcggtg
      241 gccagagact gctttgcaat gttgtttacg cttacaaata ctcctccagc ttccggaatc
      301 ttttctcccg cgccaatcac tgcctttacg tatgcttctt cgaatgtggg tgcaagtccc
      361 atgacttcgc ccgttgattt catttccggg ccgaggattg tatctgcacc aggaaagcga
      421 aaaaatggaa tcactacttc tttgactgcc acaaatccag ttgtcttgcc ggagagcatg
      481 gcgggtggga tcacttctga aatcgatttt cccataagaa gctgtgtagc tgcctgtgct
      541 agaggaagtc caatggcctt acttacaaat ggaacggtgc gagaggcgcg gggattcact
      601 tcgatgatgt aaaggcgatt ttcctgaatg gccatctgga tgttgatgag tcccaccact
      661 cctagctcga gtgcgagcat tctcgtggcg gtcttgattt cttcaatcat cgcaggagaa
      721 agagaaggag gcggaatcac gcaggcagag tcccctgaat gaacccccgc ttcttcaatg
      781 tgttgcagga ttcccgcagt gaaaacttct ttgccatccg aaagagcgtc tacatcgagt
      841 tcttctgctt cctcgaggaa tcgatcgatg agaattggat gttcaggatt gatctgttct
      901 gcttctttta cgaacttctc aagcccggca cgatcatgaa cgagcgccat cgcacggcca
      961 ccgagaacgt aactggggcg aacgagcacc ggatatccga ttctctctac cacagcttga
     1021 gcttcttcta gattgtaggc cattccattt tcaggctgaa gaagtccaag tttcttgatc
     1081 atttcgctga aacgatctct gtcctctgcg cgatcgattg catccggcga tgttccgagg
     1141 atgcgaacgc ctgcagcttc gagtccacga gcgagcttga gcggagtctg tcctccaaac
     1201 tgaacaatga ctccgtcagg tttttctaat tcgcagagat gaagcacgtc ctccgcggtg
     1261 agcggttcaa aatatagctt acttgatgtg tcgtagtctg tggaaaccgt ttctgggttt
     1321 gagttcacca tgatggactc aattcccata tcctgcagtg cgtaagatgc atggcagcag
     1381 caatagtcga attcgattcc ctgaccaatg cggttgggtc cgccgccgag aatgatcacc
     1441 ttctggttgt tagttggtgc agactcgttc atctcttcgt aggtggagta taaatatggc
     1501 gtgtgcgctt cgaattcacc ggcgcatgta tctactcttt tat
//
LOCUS       DAORFF010000718         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_76534_length_1532_cov_4.759648, whole genome shotgun sequence.
ACCESSION   DAORFF010000718 DAORFF010000000
VERSION     DAORFF010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76534_length_1532_cov_4.759648"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..686
                     /locus_tag="PKK76_19505"
     CDS             18..686
                     /locus_tag="PKK76_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNL70723.1"
                     /translation="MRILVVEDEDDIAQLIRFNLEEEGFLVTISGNGLDALSKLERNL
                     PDAIILDLMLPGMGGVEILKKIRAKFDVPVVLVTARAGETDAVLGLEMGADDYVRKPF
                     SPRELVARVKAVFRRKGTLRETATGSIERGGIEMDLSAHRVRVHGKEADLTLIEFKLL
                     QLLMENSDMAFTRDRLLDRVWGREIYVSDRAVDVNIKRLRSKLGEEKERLETVRGVGY
                     RFRG"
     gene            695..>1532
                     /locus_tag="PKK76_19510"
     CDS             695..>1532
                     /locus_tag="PKK76_19510"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNL70724.1"
                     /translation="MNIVSRILSVAFPFFVLLPFGAGLWLKKEPGSMDFAYFFYAYLA
                     ALLLAVYFAVRTTRDIALPLRALLSRIASYPVKQPNTRNVSPVTEVAAVVRSTDDFME
                     RLKVQVTDLTIEKELLGALLNGLREGVLCMDTQGTIIFQNESVDTLLIETGAPGKPYF
                     KVVRSPDLLEQMKEALSTGEQKRGDFSIGGKHFNTVCSPVLLQGKSELFIVIAYDDSQ
                     EHNARRMREDFLQNASHELKTPITSIRGYAETLLERTDDAQKASFLAAVLRNVQRMER
                     LIE"
BASE COUNT          423 a          381 c          396 g          332 t
ORIGIN      
        1 cccagaactt taaaggaatg cgaatcctcg tcgtagaaga tgaggacgac atcgcacaac
       61 tcattcgatt caatctcgaa gaggaaggat tcctggttac tatttcaggc aacggcctgg
      121 acgccttaag caagctggaa cgaaatctgc cagacgcaat catactcgat ctaatgcttc
      181 ctggaatggg cggcgtggaa atcctcaaaa aaatccgtgc gaagtttgat gtgcccgtcg
      241 tgctcgtcac tgcccgtgcc ggagaaacgg acgcagtgct gggcctcgaa atgggtgcgg
      301 acgactatgt gcgaaagccg ttcagtccgc gagaactggt ggctcgcgtc aaagcagtct
      361 ttcggagaaa aggaactctc cgcgaaaccg ctactggcag cattgaaaga ggcggaatcg
      421 aaatggatct atccgctcac agagtgcgcg tgcacggcaa agaagcagat ctcacattga
      481 tcgaattcaa actcctacaa ctgttgatgg aaaattcaga tatggctttc actcgcgatc
      541 gccttctgga ccgggtctgg ggaagagaaa tctacgtctc agaccgcgcc gtggacgtaa
      601 acatcaagag actcagatcc aaactgggtg aagaaaaaga aagactggaa actgtgcgcg
      661 gcgtaggata tcgattccgg ggttaaccgg tttcatgaac atagtcagta gaatcctatc
      721 tgttgcgttt ccgttttttg tactgctacc ctttggcgca ggcctctggt taaaaaaaga
      781 acctggaagt atggatttcg cctacttctt ctacgcttac ctggcagccc tgttgcttgc
      841 agtctatttc gcagttagaa ccactcgcga cattgcactt ccacttcgcg ctctcctttc
      901 gcgcatcgca agctacccgg tcaagcaacc gaacacccgg aatgtatccc cggtcacaga
      961 agtagcagcg gtcgtgcgaa gtacagacga cttcatggaa agactcaagg ttcaggtaac
     1021 agatctgacc atagagaaag agctgctcgg ggcattgctc aacggacttc gcgaaggtgt
     1081 cctctgcatg gacacgcagg gaaccatcat atttcagaac gagagtgtag ataccctgct
     1141 cattgaaaca ggcgcacccg ggaaacctta cttcaaggtg gtgcgcagcc cggatctact
     1201 ggagcaaatg aaggaagctc ttagcaccgg cgaacagaag agaggcgact tctccattgg
     1261 aggaaagcat ttcaacacgg tttgttctcc cgttttattg cagggaaaat cagaactatt
     1321 catagtgatc gcctatgacg attcgcagga acacaatgcg cggcgaatgc gcgaagactt
     1381 tttacagaac gcaagtcatg aactgaaaac gcccatcacg agcatccgag gatacgctga
     1441 aactttactg gaaagaacag atgatgcaca aaaagcgagt ttcctcgccg cagttcttcg
     1501 caatgtgcag agaatggagc gactcatcga gg
//
LOCUS       DAORFF010000719         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_77633_length_1521_cov_2.992497, whole genome shotgun sequence.
ACCESSION   DAORFF010000719 DAORFF010000000
VERSION     DAORFF010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77633_length_1521_cov_2.992497"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKK76_19515"
     CDS             <1..231
                     /locus_tag="PKK76_19515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70725.1"
                     /translation="TSEKELIRHYGMTFRVRFQDKVTPELYGALYYQDAKENKYCMAL
                     PDGRFAVFHDSQLAEHTMNAAQEVCYPIMACN"
     gene            complement(253..1290)
                     /locus_tag="PKK76_19520"
     CDS             complement(253..1290)
                     /locus_tag="PKK76_19520"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNL70726.1"
                     /translation="MNSIWSKIKSNPGLSLLSANVVLLAVIVLMQDPFGLFKTGYAGA
                     RVLLSASQDSISTIEVESPDKPGILRITRKDKLPVKKGEAPEYTWDVERTDGTKTTRM
                     SADRDRVKELFASLEKTRRYYGIARNSESEKDAEMATDSKGRLQCLQVRMISASGKKN
                     TVYVGKSSLRGNDSYVRLDDEDSIYLVEENLRTAFGSGDLEYFRNRRVFPDMSKDDIT
                     GITASASGRPLQIARNGQGWQMLSPVPGNVNGSEMNLLLGDFVEWKAVSFPTEIPKDI
                     DKKKSLKVEVQFKTTMTEPRTFAFEVLGQKDFSSYIIRTADGNLFEVTSIYLSHLLEP
                     EEKLLERAEKR"
     gene            complement(1287..>1521)
                     /locus_tag="PKK76_19525"
     CDS             complement(1287..>1521)
                     /locus_tag="PKK76_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70727.1"
                     /translation="NLVEAAQGDNDLAAARARVGGIDYLRPLFSRDDTLQAGFEKLFM
                     WFHILFLPVLLGIYGARRLALRGRRRGLEEVAK"
BASE COUNT          387 a          402 c          346 g          386 t
ORIGIN      
        1 acttcagaaa aagaactgat ccgccactat ggaatgacat ttcgtgtgcg cttccaggac
       61 aaagtgactc ctgaattgta cggagcgctc tactaccagg atgcaaagga aaacaaatac
      121 tgcatggcac ttccagatgg aaggttcgca gtgtttcatg attcccagct ggcagagcac
      181 accatgaatg cggcacagga agtgtgctat ccgatcatgg cgtgcaacta acagctgcaa
      241 ggcgcctgag ccctatcgct tttcagcgcg ctcgagcaac ttctcttcgg gctcaagcaa
      301 atgagacagg taaatggaag ttacctcaaa aagatttccg tctgctgtcc taatgatata
      361 ggaactgaaa tctttttgcc ccagcacttc aaaggcaaat gtgcgaggct cagtcatcgt
      421 tgttttgaat tgaacttcga cctttagact tttcttttta tctatatcct ttggaatttc
      481 agtgggaaag gaaaccgcct tccattctac aaaatcgccc agaagcaaat tcatttctga
      541 accattgacg ttcccaggaa ccggagaaag catctgccac ccctggccat ttcgcgcgat
      601 ctgaaggggc ctgccagaag cagacgcagt gattccggtg atatcgtcct tgctcatgtc
      661 cgggaatact ctgcgatttc tgaaatactc caggtccccc gatccaaatg cagtgcgaag
      721 attttcttct acgagataga ttgaatcttc atcgtccagg cggacgtacg aatcatttcc
      781 tcgcaaactg gacttcccca cgtacacagt attcttcttt ccgctcgcag aaatcattcg
      841 aacctgcaga cactgaagcc tgcccttgct gtccgttgcc atttctgcgt ctttttcgct
      901 ttcagaattt cgtgcgattc cataatagcg acgagtcttt tccagacttg caaagagttc
      961 tttgactcga tcgcggtccg cggacattcg agtggtcttt gttccgtccg ttctctccac
     1021 atcccaggtg tattcaggag cctctccttt ttttacggga agcttgtcct tgcgagtgat
     1081 gcgaagaatg ccaggtttgt ccggagactc cacttcaatc gtagaaatgc tgtcctggga
     1141 agcagacaac agaactcgcg caccggcata acctgtcttg aatagaccga atggatcctg
     1201 catcaagacg atcactgcca gaagtacaac gttagccgac aagagagaaa ggccaggatt
     1261 cgacttgatt ttagaccaga tggaattcat ttcgcaacct cttccagtcc tcttctacga
     1321 ccgcggagtg ccagtcgccg ggcgccgtaa ataccaagga gcacaggtaa aaacaggatg
     1381 tgaaaccaca tgaatagttt ttcaaaccca gcctgcaacg tatcatcgcg cgaaaacaga
     1441 ggtctgaggt aatcaatgcc gcccacgcgc gctcgagcgg ctgccagatc gttgtctccc
     1501 tgagctgctt ctactagatt g
//
LOCUS       DAORFF010000720         1514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_78332_length_1514_cov_4.152159, whole genome shotgun sequence.
ACCESSION   DAORFF010000720 DAORFF010000000
VERSION     DAORFF010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78332_length_1514_cov_4.152159"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PKK76_19530"
     CDS             <1..273
                     /locus_tag="PKK76_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70728.1"
                     /translation="LINYGMATGTTALGLMLLGLLRSHTKDEARLVYGMAVPFNAPFV
                     GGGILSLTLPEWTARGGETIIPFLLLAWMFVFFVVCRRLQQKEAHE"
     gene            266..1033
                     /gene="ygiD"
                     /locus_tag="PKK76_19535"
     CDS             266..1033
                     /gene="ygiD"
                     /locus_tag="PKK76_19535"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNL70729.1"
                     /translation="MSKMPVLFVGHGNPMLVLEDNEITQAWATLGQSLKPRAILAVSA
                     HWVTRGTLVTSMQKPRIIYDFYGFPDELYQVTYPAPGSPELALRTASLTGARADEQWG
                     LDHGTWCVLKKMFPNADIPVVQLSLDAAKSGEQHMAIGRELRELRNEGVLILASGNIV
                     HNLGAADFSGRRGTYEWATRFDNTVTSLLDQREFSKLCAIESLGNDAALSAPTPEHYW
                     PLLYALGAAEEQDRLTYPVTGMDLASISMRSVMFSES"
     gene            complement(1046..>1514)
                     /locus_tag="PKK76_19540"
     CDS             complement(1046..>1514)
                     /locus_tag="PKK76_19540"
                     /inference="COORDINATES: protein motif:HMM:NF028211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNL70730.1"
                     /translation="KDVRTDREPLHGYPTFGASSTEIWEQIALMNHRVPDNVLNHLYT
                     LYGTDYQEVLEIARKEPSLLKRISDEPGRYDIMAQIVYAVENESAHTLSDILSRRLAL
                     GSFGYPGDTAAKRAADLAGKLLKWSSSRKTEELKAYRLSYPLERLSTDSKKKK"
BASE COUNT          342 a          377 c          397 g          398 t
ORIGIN      
        1 ctgatcaact acggaatggc caccgggacc acagctcttg gtttgatgct tcttggattg
       61 ttgcgttcgc acacgaaaga cgaagcgaga cttgtatacg gcatggcagt tccgttcaac
      121 gccccattcg tcggaggtgg aattctttct ctcactcttc cagagtggac tgcgcgcgga
      181 ggcgaaacaa tcattccttt cttgctcctt gcgtggatgt ttgttttctt tgtagtgtgc
      241 agacgattgc aacagaagga agcccatgag taagatgcct gttttgtttg tagggcatgg
      301 aaatccaatg ctcgtccttg aggacaatga gatcacgcaa gcctgggcta cgctcgggca
      361 gagcctgaaa cctcgcgcca ttctagcggt ttccgcgcac tgggtcacac gcggaacgct
      421 cgtgacctcc atgcagaagc cccgcatcat atatgatttt tatggatttc ctgatgaact
      481 atatcaggtg acttatcctg cacccggctc acctgagctt gctcttcgca cagcatcttt
      541 gactggagcg cgcgcggatg aacaatgggg actggatcac ggtacctggt gtgttcttaa
      601 gaaaatgttt ccgaacgctg acatccctgt ggtgcagctg agcctggacg cggccaaaag
      661 cggagaacag catatggcga tcggtcggga attgcgcgaa ttgcgcaacg aaggagtgct
      721 gattctcgca agtggcaaca ttgttcacaa tctgggtgca gctgatttta gcggacgcag
      781 aggaacctat gaatgggcca cgcgttttga caatacagtc acttccctgc tggaccaacg
      841 agaattttcg aagttatgcg ccattgaatc gcttgggaac gacgccgctc tatctgcacc
      901 cacaccggaa cactactggc ctcttctgta tgctctggga gccgcggagg aacaggatcg
      961 cctaacctat ccagttacag ggatggatct tgcatccatt tccatgcgat ccgtgatgtt
     1021 ttccgaatct tgactcgatc ggggattatt tctttttctt cgaatcagta gaaagtcttt
     1081 cgagaggata agacaatctg tatgccttga gttcttctgt cttcctggaa gaactccact
     1141 tcagaagttt gcctgcaagg tctgccgctc gctttgccgc agtatctccg gggtagccaa
     1201 agcttccaag cgccagtctc ctggaaagaa tgtcagacag agtatgggcg gattcatttt
     1261 ctacagcgta gacgatctga gccatgatgt catatcttcc gggctcgtca gaaattcgtt
     1321 tcagcagaga aggttctttt cgcgcaatct ccagcacttc ctggtagtcg gttccgtaaa
     1381 gcgtatagag atgattgaga acgttatccg gcactctgtg attcatgagg gcaatctgtt
     1441 cccatatttc ggtggaagaa gcaccaaacg taggatatcc gtgcagtggc tcgcggtctg
     1501 tgcgaacatc tttg
//
LOCUS       DAORFF010000721         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_78458_length_1513_cov_2.901920, whole genome shotgun sequence.
ACCESSION   DAORFF010000721 DAORFF010000000
VERSION     DAORFF010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78458_length_1513_cov_2.901920"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            399..902
                     /locus_tag="PKK76_19545"
                     /pseudo
     CDS             399..902
                     /locus_tag="PKK76_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020784592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
     gene            complement(963..1253)
                     /locus_tag="PKK76_19550"
     CDS             complement(963..1253)
                     /locus_tag="PKK76_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70731.1"
                     /translation="MKILFTAILALAALTALPTSVFADKCVLCGSGSTNGCQQCRLSN
                     GVDNQANRKACESRGCKITGTGSCSTAANVKVCHAPSGSQQDVTFAWPAPAR"
BASE COUNT          355 a          355 c          402 g          401 t
ORIGIN      
        1 ccacatcgcg acgaggctga cgatcatcga ccaaatcttt cccatatcac cgctacgatc
       61 ggcgtggaag cgatgcttgc actgtcatcg gggggaaaaa tccaatcaag attggaatgc
      121 gtgaagaact ggtaagaccg gaagatgcag catcgctcaa agcccgaggc cttgattttg
      181 taatttcaaa gaatcaaact ccgtcgtcga attccggtct tcctgtaatt ggagcggatc
      241 tgctcagtga cgtttctctc ttcgcgcccg aggaattaga aaccgcagtg cctcagatga
      301 aaagtgcaaa gatggtttct attcatacaa atgagcagga tatcaccagg tctacgaggc
      361 ttgctgcggc ggcgatgaaa cagaatggtt tgaatctacc catcattctg tttgccaggt
      421 tctcctcgct tgaggaagct ctttatcaag cttccattca cttcggagga ttgcttctcg
      481 acgggatcgg agacgcactg gtcatagaca tagcgggtgc cggtccggat caggcaagtc
      541 atctgttgga tttgtccctg gatattttgc aggctaccag acttcgtctt tcgaaaactg
      601 aattcatcag ctgtccgtcc tgcggcagaa cgttattcga tcttcaggat acaaccgcca
      661 gaattcaaaa gcgcactggc catctcaaag gtgtaaagat cgccattatg ggttgtgttg
      721 tgaacgggcc cggggaaatg gcagatgcgg attttggtta tgtgggtgcg gggcctggaa
      781 agattcatct ttatcgcggt aaggaaatag ttaagaaaag tataccatct gagattgcag
      841 atgaagagct ggtgcgcctg atccaggagc acggaatgtg ggtggagcca ggagcaggtt
      901 gagcactgga ttatggaaag gagagggacg tttgcacgtc cctccaatgg atcgactggt
      961 tgttatcgcg cgggcgcagg ccacgcaaag gttacgtctt gctggctccc ggaaggagcg
     1021 tggcaaactt ttacattcgc agcagtggaa caggatccag tgcctgtgat tttgcatcct
     1081 ctggattcgc atgctttgcg atttgcctgg ttgtctacac cgttagacaa tctgcattgc
     1141 tggcatccgt ttgtgcttcc gcttccgcag agcacgcatt tgtctgcgaa aacgctggtt
     1201 ggcagtgctg tgagtgctgc tagtgccagg atcgccgtga acagtatctt catgttatcc
     1261 cccgcaagat ccaaatgatt gaatcttcgc attgtttgcc aatttttgtg gttcgcgttt
     1321 gcagtcgtct actggttttt gatatcccgc ggagttaaaa aaaaactgtt tcataacatg
     1381 atcttgcgac tcattccaag aaaagatcgt ccaaccgcag atgaggaagt ttcttctatt
     1441 tttcctggct cttgccggat gcatccctga accgcggcgc attccagggg atccactgga
     1501 ttctcctctg cta
//
LOCUS       DAORFF010000722         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_78641_length_1511_cov_3.671703, whole genome shotgun sequence.
ACCESSION   DAORFF010000722 DAORFF010000000
VERSION     DAORFF010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78641_length_1511_cov_3.671703"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PKK76_19555"
     CDS             <1..227
                     /locus_tag="PKK76_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HNL70732.1"
                     /translation="RNLEKHVEELELSVRSLSVLKSLEIETLADLVRRNEEELQKSRH
                     YSPNCVEEIRQKLAGLNLEFGMRDFFAPRD"
     gene            239..778
                     /gene="rplQ"
                     /locus_tag="PKK76_19560"
     CDS             239..778
                     /gene="rplQ"
                     /locus_tag="PKK76_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002735277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNL70733.1"
                     /translation="MRKRNKTKQLNRVSSHRKAMMRNMATSLFDHERIISTRAKVKVL
                     RPYAEKLITRAKHTVSGEMNEAQVVHSKREILKHVRDRDVAVKLLQDIAPRFKERNGG
                     YLRIIHLPERDKDSAKMSIIELVERKEKVRKQKPEKERKASSSPEKGKDKKSLPSEKD
                     AKKEKWYNRFRKKKSDVPS"
     gene            complement(811..1215)
                     /locus_tag="PKK76_19565"
     CDS             complement(811..1215)
                     /locus_tag="PKK76_19565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNL70734.1"
                     /translation="MRITSRGRYGLKAMMELSSGGLLKTREISERQNIPQKYLEQIVH
                     LLRKNGLVSSIRGAEGGYRLTRSASEITILEILEALEGDLSLIDRSDKTWDESTGGFW
                     KELESRLHEMLSVSLAEFMASGSKKGELMYYI"
     gene            complement(1215..>1511)
                     /locus_tag="PKK76_19570"
     CDS             complement(1215..>1511)
                     /locus_tag="PKK76_19570"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNL70735.1"
                     /translation="RAMWLMLQQDKADDYVISTGETHSVQELVEVAFSHAGLDWKKHV
                     KIDPAFIRPAEVDLLIGDASKAKKELGWVPEVSFEGLVKMMVDADIERLKNGRP"
BASE COUNT          401 a          371 c          378 g          361 t
ORIGIN      
        1 gccgcaatct ggagaagcat gtagaagaac tcgaactttc tgttcgctct ctttctgtgc
       61 tcaagagcct ggaaatcgaa actctcgcgg atctggtccg cagaaacgaa gaagagcttc
      121 agaaatcacg tcactattcc ccgaactgcg tagaagaaat tcgccagaaa cttgcgggac
      181 tcaatcttga attcggaatg cgcgactttt ttgcgcctcg cgactaaggc ctgacatcat
      241 gagaaagaga aacaaaacaa aacaactgaa tcgcgtatct tcgcacagaa aagcgatgat
      301 gcgcaacatg gcaacgagct tgtttgacca tgagagaatc atttctacgc gtgcgaaggt
      361 aaaagtcctt cgtccgtacg cggaaaagct gatcactcgc gcgaagcaca ctgtgtccgg
      421 cgaaatgaac gaagcgcaag tggttcacag caagagagaa attctgaagc acgttcgcga
      481 tcgcgacgtg gctgttaaac ttcttcagga tatcgcgcct cgtttcaagg aacgcaacgg
      541 tgggtacctg cgcatcattc atcttccaga acgcgacaaa gatagcgcga agatgagcat
      601 catcgaactg gtggaacgca aagagaaggt tcgcaagcag aaacctgaaa aggaaagaaa
      661 agcttcatcc tcaccggaga aaggtaagga caagaagagc cttccaagcg aaaaagacgc
      721 caagaaagag aagtggtaca atcgcttccg taaaaagaaa tcagacgttc caagctgatt
      781 ggagaaaggc cgcgaaagcg gccttttttg ttagatgtag tacattagtt cgcctttttt
      841 agaaccagag gccatgaatt ctgccagaga tacgctgagc atttcatgaa ggcgagactc
      901 cagttctttc cagaagccac ccgtagattc atcccatgtc ttgtctgagc gatcaatgag
      961 ggatagatct ccttcgagag cttcgagtat ttcaagtatt gtgatttcag aagcagagcg
     1021 agtgagtctg taaccgcctt ctgcgccgcg gatgctcgat accagcccgt tctttcgaag
     1081 gaggtgcacg atttgctcga ggtatttttg cgggatgttc tgcctttccg agatttctct
     1141 tgttttcaag agcccgcccg acgacaattc catcatcgct ttgaggccgt agcggccgcg
     1201 gcttgtgatt ctcactatgg ccggccgttt tttagtcttt caatatccgc gtcgaccatc
     1261 atcttcacga ggccttcaaa gctcacttct ggaacccagc cgagttcctt ttttgccttg
     1321 gatgcatctc cgatgagtaa atccacttcg gcgggacgaa tgaatgcagg atcgatcttc
     1381 acgtgcttct tccagtccag gcctgcatgg gagaatgcta cttctacaag ttcctgcacg
     1441 ctgtgtgttt ctccggtgga gattacgtag tcgtccgctt tatcctgctg gagcatgagc
     1501 cacatggcgc g
//
LOCUS       DAORFF010000723         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0835
            NODE_79152_length_1507_cov_2.063361, whole genome shotgun sequence.
ACCESSION   DAORFF010000723 DAORFF010000000
VERSION     DAORFF010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852178
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:33:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,917
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,869
            CDSs (with protein)               :: 3,869
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79152_length_1507_cov_2.063361"
                     /isolate="AMR_MDS_0835"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PKK76_19575"
     CDS             <1..637
                     /locus_tag="PKK76_19575"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNL70736.1"
                     /translation="RGREGFYFIYYLDRKEQTIKGGFNYIELKQDKPVRMMQANSGKF
                     DPATGEWTFSRVRNISFGDDLAVLKVENEDTRVEKFPEDYDFFENPMRDASELNLFEL
                     GDEIARKKKMRFDSAPYSVQFHSLLAFPFLCMIVAVIASIAGAGGNHRSTGPLIRALL
                     VSAVTILIYYLGSGLFNNLGNNGVIYPWLASWTPTVGFFCAAVFLILRNRK"
     gene            634..1041
                     /locus_tag="PKK76_19580"
     CDS             634..1041
                     /locus_tag="PKK76_19580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNL70737.1"
                     /translation="MSVIYRHYADAEQFAQYIKGKWRGAGTHGRADWPEDTEIDLKIE
                     SASFVRLRGVRRKKESGLKRLLARIRGTGEAAFDLSVRGVTDARTEADLAVVTSGIEK
                     ITLQAMTEDRCHVTIERTGDRPLLMFSGVVHRK"
     gene            1038..>1507
                     /locus_tag="PKK76_19585"
     CDS             1038..>1507
                     /locus_tag="PKK76_19585"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNL70738.1"
                     /translation="MKYYRDLRDFIKVLEEENELVRIKTLVSRDLEISEIYFRQIRSP
                     GGGKALLFENVEGSNLPVLINAFGSKKRMDLALGRRTSREGAPGKVDQIAEELRGLMR
                     LMQFKPPKTAGEIASLAGKAAGILRYPPRRRRFFKAPCQEIVLTGDQIDLDQIP"
BASE COUNT          374 a          334 c          426 g          373 t
ORIGIN      
        1 gcgcgggcgc gaaggcttct atttcattta ctacctggat cgcaaggagc aaacgatcaa
       61 aggtgggttc aactacatag aactcaagca ggacaaacct gtcagaatga tgcaggcgaa
      121 ttcaggcaaa tttgatcctg cgactggcga atggactttc tcgcgcgttc gcaatatatc
      181 tttcggggat gacctggcag ttctcaaggt ggagaatgag gataccagag tagagaaatt
      241 cccggaagat tatgacttct ttgaaaaccc gatgcgcgat gcatccgagc tgaatttgtt
      301 tgagcttgga gatgaaattg cgcgaaagaa gaagatgcgg tttgattctg ccccgtactc
      361 tgttcagttc cattctcttc ttgcgtttcc gtttctgtgc atgattgttg ctgtcatcgc
      421 ttcgattgca ggggcgggtg gaaaccacag gtctaccggg cctttgattc gggcactcct
      481 ggtgtctgcc gtcaccattt tgatctacta tcttggctct ggccttttca acaacctcgg
      541 aaacaatgga gtcatctatc cctggcttgc gtcgtggacc cccaccgttg gattcttctg
      601 tgccgcggtc ttcttgattt tgaggaatcg taaatgagtg tcatctacag gcattatgcg
      661 gacgcagagc aatttgcgca gtacatcaaa ggaaagtggc gtggagcggg aactcacggc
      721 agggcagact ggccggagga tacagaaatc gatctcaaaa ttgaatcagc ctcctttgtt
      781 cgcctgcgcg gtgtaaggcg aaagaaggaa tccggattga agcgacttct tgcgcgcatt
      841 cgagggacag gagaggcagc atttgattta tctgttcgcg gcgtgacaga tgcgcgcaca
      901 gaggcggacc tggcagtggt aaccagtggc atagaaaaga ttacgctgca ggccatgaca
      961 gaggatcgat gccatgtcac catcgagcgg acaggagaca ggccattgtt gatgttttca
     1021 ggcgttgttc atagaaaatg aagtactaca gagatttaag agattttatc aaggttctgg
     1081 aagaagaaaa cgagcttgtt cgaatcaaga ctctggtgtc acgcgatctg gagatatcag
     1141 aaatctattt cagacagatt cgttctcctg gtggtggaaa ggccctcctg tttgagaatg
     1201 tcgaaggatc caatcttcct gtgttgatca atgcgttcgg atccaaaaag agaatggatc
     1261 tggcactggg caggcgcact tcgcgagagg gcgctccagg gaaggtagat cagattgcgg
     1321 aggaactgcg agggctcatg cgcctcatgc agtttaagcc tcctaagact gcaggggaaa
     1381 tcgcgagtct cgcaggcaag gcggctggca tcttacgtta tccgcccaga cgaagacgtt
     1441 ttttcaaagc gccctgtcag gagattgtgc tgacaggaga tcagatcgat ctggatcaga
     1501 ttccgat
//
